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Sample records for blanco litopenaeus schmitti

  1. Population genetic structure of Brazilian shrimp species (Farfantepenaeus sp., F. brasiliensis, F. paulensis and Litopenaeus schmitti: Decapoda: Penaeidae

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    Jaqueline Gusmão

    2005-03-01

    Full Text Available Penaeid shrimps are important resources for worldwide fisheries and aquaculture. In the Southwest Atlantic, Farfantepenaeus brasiliensis, F. paulensis, F. subtilis, Farfantepenaeus sp. and Litopenaeus schmitti are among the most important commercially exploited species. Despite their high commercial value, there is little information available on the different aspects of their biology or genetics and almost no data on their stock structure. We used allozymes to estimate variability levels and population genetic structure of F. brasiliensis, F. paulensis, L. schmitti and the recently detected species Farfantepenaeus sp. along as much as 4,000 km of Brazilian coastline. No population heterogeneity was detected in F. brasiliensis or L. schmitti along the studied area. In contrast, F ST values found for Farfantepenaeus sp. and F. paulensis indicate that the populations of those two species are genetically structured, comprising different fishery stocks. The largest genetic differences in F. paulensis were found between Lagoa dos Patos (South and the two populations from Southeast Brazil. In Farfantepenaeus sp., significant differences were detected between the population from Recife and those from Fortaleza and Ilhéus.

  2. Total carotenoids and antioxidant activity of fillets and shells (in natura or cooked of “Vila Franca” shrimp (Litopenaeus Schmitti in different intervals of storage under freezing

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    Giselda Macena Lira

    Full Text Available ABSTRACT Shrimps are sources of carotenoids, astaxanthin is the predominant, responsible for their special and desirable properties, as well as for their instability under heat treatment during the domestic preparation, industrial processing or storage under freezing. These can cause discoloration and reduce the beneficial health properties. This study aimed to evaluate the effect of heat treatment and storage under freezing (0, 45 and 90 days on the levels of total carotenoids and stability of the antioxidant activity of ethanolic extracts of fillets and shells, raw and cooked, of the white shrimp (“Vila Franca” Litopenaeus schmitti (Burkenroad, 1938. The antioxidant ability of the extracts was evaluated using the radicals DPPH• (2,2-diphenyl-1-picryl-hydrazyl and ABTS+• (2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid, as well as by the iron reducing power (FRAP test. The extracts of cooked or in natura shrimps (fillets and shells represent dietary sources of carotenoids, displaying antioxidant activity through all the tested methods, after heat treatment and storage under freezing. The antioxidant activity of the extracts was superior to the one of ascorbic acid, mainly in the cooked fillet and shells. The samples of shrimp shells seemed a valuable source of carotenoids, whose antioxidant activity was verified even 90 days after freezing, and can be used in food products as functional natural supplement, adding value to this waste.

  3. ACUICULTURA EPICONTINENTAL DEL CAMARÓN BLANCO DEL PACÍFICO, Litopenaeus vannamei (Boone, 1931.

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    Daniel Enrique Godínez Siordia

    2010-11-01

    Full Text Available Se hizo una revisión sobre el estatus del cultivo de camarón Litopenaeus vannamei en agua de baja salinidad, presentando características, ventajas, desventajas y medidas de mitigación sobre el impacto ambiental de este sistema de cultivo. Este trabajo aborda aspectos relevantes con la finalidad de trazar un camino hacia la sustentabilidad de esta alternativa de cultivo que se expande rápidamente en México.

  4. Efecto tóxico de DDT y endosulfan en postlarvas de camarón blanco, Litopenaeus vannamei (Decapoda:Penaeidaede Chiapas,México

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    Vicente Castro-Castro

    2005-06-01

    Full Text Available Con el fin de conocer la toxicidad del DDT y endosulfan sobre postlarvas de camarón blanco (Litopenaeus vannaei, se realizaron pruebas de toxicidad aguda en condiciones de laboratorio por 168 h, con temperatura de 29 ± 1 °C, salinidad de 3 ± 1 ‰ y pH en 8 ± 1.Se calculó la concentración letal media (LC50 , la LC50 "incipiente", los tiempos medios de muerte (LT50 , la Máxima Concentración Aceptable del Tóxico (MACT y el "Nivel de Seguridad" (LS; así mismo, en los organismos sobrevivientes se determinó la concentración a la que el crecimiento de los organismos se reduce en un 5 y 50% (CE5 y CE50 . Se evaluaron además las alteraciones en el consumo de oxígeno. El DDT fue 3 veces más tóxico que el endosulfan; sin embargo, los organismos resultaron ser muy sensibles a ambos compuestos. La tasa de crecimiento de las postlarvas disminuyó en un 80 y 50% para el DDT y endosulfan respectivamente. La baja resistencia de las postlarvas al DDT y endosulfan, y las concentraciones de estos compuestos en la laguna, sugieren que si se diera un ingreso adicional de estos plaguicidas al sistema, es muy probable un potencial impacto en la producción de camarón del sistemaToxic efect of DDT and endosulfan in white shrimp postlarvae Litopenaeus vannamei (Decapoda: Penaeidae from Chiapas, Mexico .We analized acute toxicity in white shrimp (Litopenaeus vannamei postlarvae exposed to two chlorinated pesticides, DDT and endosulfan, under laboratory conditions during 168 hours, with controlled temperature (29 ± 1°C, salinity (3 ± 1 ‰ and pH (8 ± 1. Median lethal concentrations (LC50 , "incipient" LC50, median lethal time (LT50 the "maximum acceptable concentration of the toxic compound" (MACT and "the safety level" (SL were determined. The concentration of the compounds at which organism growth was reduced by 5 and 50% (EC5 and EC50 , as well as changes in oxygen consumption patterns were determined in the surviving postlarvae.They were very

  5. Respuesta inmunomoduladora de la superóxido dismutasa en juveniles de camarón blanco (Litopenaeus vannamei expuestos a inmunoestimulantes

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    2005-01-01

    Full Text Available Se estudió la actividad inmunomoduladora de la manganeso superóxido dismutasa (MnSOD en juveniles de camarón blanco (Litopenaeus vannamei expuestos a diferentes inmunoestimulantes. Organismos cuyo peso varió entre 0.7 y 1.0 g fueron inmersos durante 6 h en soluciones de β-glucano, lipopolisacárido (LPS, fucoidán, y Vibrio penaeicida muerto por calor. Se determinó la actividad enzimática de la MnSOD en los organismos para evaluar si los diferentes inmunoestimulantes utilizados eran capaces de inducir actividad antioxidante. Los inmunoestimulantes probados activaron el sistema inmune de los camarones, mostrando un incremento generalizado en la respuesta antioxidante 48 h después del ensayo. El mayor incremento en la actividad de la enzima (3.2 veces superior al grupo control se registró cuando se utilizó la bacterina. Se expusieron los camarones a V. penaeicida 10 días después de la exposición a los inmunoestimulantes, obteniendo un incremento en la actividad antioxidante de los camarones expuestos previamente a β-glucano, LPS y fucoidán. La respuesta enzimática más alta se obtuvo con los organismos estimulados con β-glucano (2.5 veces superior al control. Este estudio demostró la capacidad de los juveniles de camarón para mejorar la respuesta antioxidante después de ser expuestos a inmunoestimulantes y a una bacteria patógena.

  6. Evaluación in vivo del efecto cicatrizante de un gel a base de quitosano obtenido de exoesqueleto de camarón blanco litopenaeus vannamei

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    Martínez Sánchez, Heimy Franceline; Escobedo Lozano, Amada Yerén; Méndez-Gómez, Evaristo; Vázquez, Alfredo Emmanuel; Sol Hernández, Manuel de Jesús; Osuna Lizárraga, Anahí Elizabeth

    2014-01-01

    Healing effect of a chitosan-based gel obtained from the exoskeleton of white shrimp Litopenaeus vannamei and its evaluation in vivoTítulo corto: Evaluación del quitosano como cicatrizanteResumen:  En México alrededor del 62 % de la población sufre de accidentes causantes de alteraciones en la piel como quemaduras, heridas y diabetes principalmente. Para atender esta problemática, se propone el uso de un gel de quitosano, obtenido del exoesqueleto de camarón ya que presenta actividad antimicr...

  7. Ecological distribution of the shrimp Nematopalaemon schmitti ...

    African Journals Online (AJOL)

    The relationships between the spatial and temporal variations in the abundance of the shrimp Nematopalaemon schmitti and water temperature, salinity, and texture and organic-matter content of the sediment, were analysed in Ubatumirim, Ubatuba and Mar Virado bays on the northern coast of São Paulo, Brazil. Sampling ...

  8. Efecto de la adición de un extracto acuoso de pionilla Lasianthaea podocephala en el cultivo del camarón blanco del Pacífico Litopenaeus vannamei en condiciones de laboratorio

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    Emmanuel Villanueva-Gutiérrez

    2015-11-01

    Full Text Available Se evaluó el efecto de dos concentraciones de un extracto acuoso de la raíz de pionilla (Lasianthaea podocephala Gray, sobre las variables de la calidad del agua, condición fisiológica y parámetros de producción del camarón blanco del Pacífico, Litopenaeus vannamei (Boone, cultivado en condiciones intensivas de laboratorio. Dos tratamientos y un control fueron evaluados por triplicado: T1 (1 mL de extracto por acuario, T2 (3 mL y C (control, 0 mL. No se observó un efecto negativo de los tratamientos sobre los parámetros de la calidad del agua, los cuales estuvieron dentro de rangos aceptables, sin presentar diferencias significativas entre tratamientos (P < 0,05. Algunos de los parámetros de producción tales como la supervivencia, biomasa final y FCA fueron mejores en los tratamientos en que se utilizó el extracto bajo las condiciones experimentales empleadas. La concentración de metabolitos hemolinfáticos, sugiere que los organismos cultivados en los acuarios con extracto tuvieron mejores condiciones, considerando los niveles mayores de proteína y colesterol en su músculo en relación con el control; además los resultados de expresión de genes indican que el extracto podría tener algún efecto inmunoestimulante sobre los camarones. No obstante, se recomienda efectuar estudios adicionales para evaluar y determinar a nivel molecular los ingredientes activos de los tubérculos de raíz de pionilla, para obtener mayor información sobre el uso potencial de este vegetal en la acuacultura.

  9. Aplicación de modelos lineales mixtos en infecciones experimentales con WSSV en el camarón blanco Litopenaeus vannamei

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    María Alejandra Ramírez-Ruiz

    2015-07-01

    Full Text Available El virus de la mancha blanca (WSSV es uno de los virus más devastadores en la industria camaronícola. Hasta la fecha no se ha hallado una cura para la enfermedad por lo que es necesario diseñar protocolos experimentales reproducibles y medibles para evaluar fármacos y respuestas fisiológicas de los ejemplares durante la prognosis de la infección. En este estudio se evaluaron dos vías de infección (inyección e inmersión con WSSV (200 copias de ADN a temperatura constante de 26 ± 0,5°C, en juveniles Litopenaeus vannamei (4,8 ± 0,38 g en estado de inter-muda. En la infección por inyección se observó nado errático, letargia y coloración rojiza a partir de las 24 h y la mortalidad fue del 100% a los 2-5 días: en 63% de los ejemplares se observó infección ligera [20 copias de ADN y 1-5 cuerpos de inclusión intranuclear (CAI/200 campos], 21% con infección moderada (200 copias de ADN y 1-2 CAI/20 campos y 16% con infección severa (2000 copias de ADN y más de 10 CAI/campo. No se observó mortalidad en los ejemplares controles. En la infección por inmersión, los signos de la enfermedad se observaron a partir del día 3, en un período de 3-9 días se observó 38% de mortalidad: 25% con infección ligera (20 copias de ADN y 1-2 CAI/20 campos, 5% con infección moderada (200 copias de ADN y 1-2 CAI/ 20 campos y 8% con infección severa (2000 copias de ADN y 1-5 CAI/2 campos. El 62% que sobrevivió, fue PCR positivo, con grado de infección ligera (20 copias de ADN pero sin inclusiones CAI. No se observó mortalidad en el grupo control. Los datos recabados no cumplían con los supuestos de independencia y linealidad requeridos para la aplicación de análisis de varianza por lo que se usaron los modelos lineales mixtos donde se observó mayor precisión y capacidad de predicción. En los ejemplares inyectados la mortalidad tuvo su máximo más alto al día 5 después de la inoculación. Mientras que en los infectados por inmersi

  10. Culture of white shrimp (Litopenaeus vannamei Boone, 1931 with zero water exchange and no food addition: an eco-friendly approach Cultivo de camarón blanco (Litopenaeus vannamei Boone, 1931 sin recambio de agua y sin adición de alimento formulado: un sistema amigable con el ambiente

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    Juan Manuel Audelo-Naranjo

    2012-07-01

    Full Text Available Juveniles (3.5 ± 0.3 g of the white shrimp Litopenaeus vannamei were grown during 40 days with no water exchanges, no food addition and four initial densities (25, 50, 75 and 100 g m-3, corresponding to between 8 and 32 shrimp m-2, to determine growth rates, which could be achieved using the periphyton growing on artificial substrates as the only food source. The experimental culture units were 12 polyethylene 1 m³ cylindrical tanks with 4.8 m² of total submerged surface (bottom and walls, provided with 7.2 m² of artificial substrate (Aquamats™. There were no significant differences in the ammonia and nitrite concentrations determined in the four treatments (0.17-0.19 and 0.10-0.11 mg L-1, respectively, which remained below the respective levels of concern for shrimp cultures. Mean survival was similar, and ranged from close to 91 to 97%, whereas there were significant differences in mean individual weight, which ranged from 11.9-10.6 g shrimp-1 for the two low initial densities (25 y 50 g m-3, to 8.3-7.7 g shrimp-1 for the other treatments. However, because of the high survival and of the higher initial density, the best biomass yield was with 100 g m-3. The final nitrogen contents of sediment and water were lower than the initial values, and between 36 and 60% of the difference was converted into shrimp biomass.Durante 40 días se cultivaron juveniles de camarón blanco Litopenaeus vannamei con un peso individual de 3,5 ± 0,3 g y biomasas iniciales de 25, 50, 75 y 100 g m-3 (equivalente a 8-32 ind m-2, sin cambios de agua y adición de alimento, para determinar la tasa de crecimiento usando como única fuente de alimentación el perifiton desarrollado en sustratos artificiales. Se utilizaron estanques cilíndricos de polietileno de 1 m³ con tres réplicas por tratamiento, con una superficie de 4,8 m² (paredes y fondo y 7,1 m² de sustrato artificial (Aquamats™. No se encontraron diferencias significativas entre las concentraciones

  11. Effect of Nile tilapia (Oreochromis niloticus on the growth performance of Pacific white shrimp (Litopenaeus vannamei in a sequential polyculture system Efecto de la tilapia del Nilo (Oreochromis niloticus sobre el crecimiento del camarón blanco del Pacífico (Litopenaeus vannamei, en un sistema de policultivo secuencial

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    Cesar Hernández-Barraza

    2012-11-01

    Full Text Available The present study was carried out at the Environmental Research Laboratory (ERL, University of Arizona, to assess the effect of the addition of Nile tilapia (Oreochromis niloticus, at different densities, on the growth performance of Pacific white shrimp (Litopenaeus vannamei. The growth rate and feed conversion of shrimp, both in polyculture and monoculture, were evaluated. Shrimp-tilapia proportions were 20:8 individuals in Treatment One (T1, 20:4 individuals in Treatment Two (T2 and 20:2 individuals in Treatment Three (T3, while in Treatment Four (T4 shrimp were stocked as a control group with a ratio of 20:0. The experiment lasted for four weeks at 10 ppt water salinity. The shrimp and fish were fed once a day with 8% and 3% of their body weight, respectively, using a 35% protein feed. At the end of the experiment, the average individual weight and best feed conversion ratio were obtained in shrimp polyculture treatment with highest tilapia density 6.08 ± 0.18 g and 1.26 ± 0.01 respectively, while the lowest scores were found in the monoculture treatment with 5.14 ± 0.59 g and 1.35 ± 0.01, respectively (P El presente estudio se llevó a cabo en el Laboratorio de Investigación del Medio Ambiente (ERL. de la Universidad de Arizona, para evaluar el efecto de la adición de la tilapia del Nilo (Oreochromis niloticus a diferentes densidades, en el desempeno del crecimiento del camarón blanco del Pacífico (Litopenaeus vannamei. La tasa de crecimiento y conversión alimenticia del camarón, tanto en policultivo y monocultivo, fueron evaluados. Las proporciones de camarón y la tilapia fueron de 20:8 individuos en el tratamiento uno (T1, 20:4 en el tratamiento dos (T2 y de 20:2 en el tratamiento tres (T3, mientras que en el tratamiento cuatro (T4, únicamente fueron sembrados camarones, participando como grupo control con una relación de 20:0. El experimento se realizó durante cuatro semanas y agua a 10 ppm de salinidad. Los camarones y

  12. Trophic ecology of Mustelus schmitti (Springer, 1939) in a nursery area of northern Patagonia

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    Molina, Juan Manuel; Cazorla, Andrea López

    2011-05-01

    Mustelus schmitti is an endangered endemic shark of the southwest Atlantic, and an important economical resource in Argentina, Brazil and Uruguay. The objective of this study was to describe the trophic ecology of M. schmitti in Anegada Bay, its feeding strategy and diet composition, along with the possible dietary shifts, due to season, sex, ontogeny and the different geographical features of the bay. Our results show that M. schmitti is a carnivorous opportunistic predator, feeding on a variety of benthic invertebrates. The diet presented seasonal and ontogenetic variations, while no differences in diet composition were observed between sexes or the different sampling sites. This species behave as a generalize feeder, with a wide trophic spectrum and a diverse diet.

  13. Antimodernistlik tegutseja modernismis : sissejuhatus Carl Schmitti riigifilosoofilisse õpetusse / Heimes. Claus ; tõlk. Peeter Helme

    Index Scriptorium Estoniae

    Heimes. Claus

    2004-01-01

    Saksa poliitilise teoreetiku, riigiõigusasjatundja ja kristliku filosoofi Carl Schmitti (1888-1985) seisukohtadest poliitilises teoloogias ja antropoloogias, uusajatõlgendusest ning positivistliku kaasaja kriitikast. Tõlke allikas: Antimoderner Akteur der Moderne : eine Einführung in die staatsphilosophische Lehre Carl Schmitts

  14. Nevus blanco esponjoso familiar

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    Mônica Andrade Lotufo

    2010-06-01

    Full Text Available El nevus blanco esponjoso (NBE es una rara condición autosómica dominante, caracterizada por placas blancas bilaterales en la mucosa, de aspecto esponjoso, blandas a la palpación y que pueden escamarse. Los tratamientos son paliativos; y el uso de antibióticos, en especial la tetraciclina, ha demostrando buenos resultados en su control. Este trabajo presenta tres casos clínicos de una familia afectada por NBE, donde se discuten los posibles diagnósticos diferenciales y conductas terapéuticas indicadas. Un paciente masculino de 52 años de edad acudió a la clínica aquejado de lesiones blancas bilaterales. El paciente notó las lesiones 30 años antes, sin lograr un diagnóstico final de las mismas. Después de la anamnesis y del examen clínico fue realizada una biopsia incisional. La reunión de los datos clínicos e histopatológicos llevó al diagnóstico de NBE. Se le solicitó al paciente que indagase entre sus familiares con respecto a lesiones semejantes. Se detectó que el hijo de 19 años y la hija de 25 eran portadores de placas blancas en la mucosa yugal. Como no había afectación estética, se optó por no intervenir en las lesiones. El nevus blanco esponjoso es una lesión genética que debe ser diferenciada de otras patologías localizadas y sistémicas importantes, que tienen repercusiones serias para el individuo. Como no hay un tratamiento curativo para el NBE, el papel del cirujano dentista es diagnosticar esta lesión, aclarar al paciente sobre la naturaleza benigna y autolimitante del NBE y si fuera necesario desde el punto de vista estético, aplicar diferentes modalidades terapéuticas.

  15. quebracho-blanco Schlecht

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    E. O. Sanabria

    2007-01-01

    Full Text Available La especie Aspidosperma quebracho-blanco Schlecht es la más abundante del Parque Chaqueño y no es utilizada en la producción de bienes de alto valor agregado debido a la inestabilidad dimensional de su madera. Una de las maneras de mejorar la estabilidad dimensional es el engrosado de la pared celular con polietilenglicol (PEG. El objetivo de este trabajo fue determinar el efecto del PEG de peso molecular 300 y 600 en la estabilidad dimensional, utilizando como parámetro de control el coeficiente de retractabilidad de la madera citada. Las muestras se impregnaron en una Planta Piloto con PEG, a través del proceso Bethell, según Norma IRAM Núm. 9511. Posteriormente se secaron hasta un contenido de humedad (CH del 10 %. Los mejores resultados se lograron impregnando la madera con PEG 600, a una concentración del 50 % en solución acuosa, a una presión de 12 kg·cm-2 durante 120 minutos, lo que permitió disminuir el coeficiente de retractabilidad de esta madera en 58.2 %.

  16. Relative growth of Acantholobulus schmitti (Rathbun, 1930 (Crustacea, Brachyura, Panopeidae at Guaratuba Bay, southern Brazil

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    SF Frigotto

    Full Text Available A study on the relative growth of the crab Acantholobulus schmitti (Rathbun, 1930 (Decapoda, Brachyura, Panopeidae was performed with allometric techniques. The species is associated with lanterns used for oyster farming at Guaratuba Bay, southern Brazil. Crabs were obtained from five lanterns from March through December 2009. The following morphological characteristics were measured: the carapace width (CW, the height and length of the major cheliped propodus (LPH and LPL, the width of the female abdomen at the base of the 4th somite (AW and the length of the male gonopod (GL. A total of 1,004 individuals of A. schmitti were measured, of which 451 were males, 323 non-ovigerous females and 230 ovigerous females. The CW of the males varied from 1.87 to 11.86 mm, that of non-ovigerous females from 1.44 to 8.77 mm and that of ovigerous females from 4.09 to 11.12 mm. The plot of LPH against CW showed a mean inflection point at 3.67 mm CW for the males. This result indicated that male crabs are juvenile below this value and adult above it. For the females, the corresponding inflection point occurred at 3.36 mm CW in the plot of AW against CW. These results, CW x LPH and CW x AW, highlight the importance of chelae enlargement at the onset of maturation in males. Similarly, the widening of the abdomen in females is required to accommodate egg clutches during the reproductive period. Heterochely was recorded in both sexes. Right-handed crabs were more frequent than left-handed ones, with percentages of 75.8% in males and 82.7% in females. The population of A. schmitti from Guaratuba Bay becomes sexually mature at an earlier age than A. schmitti from the coast of São Paulo state. The lanterns used in oyster farming furnish a safe habitat in which these crabs can spend their entire benthic life.

  17. Calliobothrium spp. (Eucestoda: Tetraphyllidea: Onchobothriidae) in Mustelus schmitti (Chondrichthyes: Carcharhiniformes) from Argentina and Uruguay.

    Science.gov (United States)

    Ivanov, Verónica A; Brooks, Daniel R

    2002-12-01

    Three species of Calliobothrium inhabit the spiral intestine of Mustelus schmitti in Argentina and Uruguay. Calliobothrium verticillatum australis is redescribed and its taxonomic status modified to species as C. australis. Calliobothrium barbarae n. sp. can be distinguished from all other species of Calliobothrium, which are small bodied, nonlaciniate, and without accessory piece between the bases of axial hook, by worm length, number of segments, cocoon morphology, and hooks shape. Calliobothrium lunae n. sp. is different from other Calliobothrium spp., which are small bodied, nonlaciniate. and have an accessory piece, by the number of segments and testes, hook shape, cocoon morphology, and the presence of ciliumlike projections on the distal surface of muscular pads. Calliobothrium australis is clearly distinguished from other large-bodied, laciniate species of the genus by worm length, number of testes, ovary shape, cocoon morphology, hook shape, and in being hyperapolytic. The oioxenous specificity involving Calliobothrium spp. and Mustelus spp. described by previous authors is confirmed in this study.

  18. Dark Energy Camera for Blanco

    Energy Technology Data Exchange (ETDEWEB)

    Binder, Gary A.; /Caltech /SLAC

    2010-08-25

    In order to make accurate measurements of dark energy, a system is needed to monitor the focus and alignment of the Dark Energy Camera (DECam) to be located on the Blanco 4m Telescope for the upcoming Dark Energy Survey. One new approach under development is to fit out-of-focus star images to a point spread function from which information about the focus and tilt of the camera can be obtained. As a first test of a new algorithm using this idea, simulated star images produced from a model of DECam in the optics software Zemax were fitted. Then, real images from the Mosaic II imager currently installed on the Blanco telescope were used to investigate the algorithm's capabilities. A number of problems with the algorithm were found, and more work is needed to understand its limitations and improve its capabilities so it can reliably predict camera alignment and focus.

  19. Echinobothrium notoguidoi n. sp. (Cestoda: Diphyllidea) from Mustelus schmitti (Chondrichthyes: Carcharhiniformes) in the Argentine Sea.

    Science.gov (United States)

    Ivanov, V A

    1997-10-01

    A new cestode, Echinobothrium notoguidoi n. sp., is described from the spiral intestine of the shark Mustelus schmitti, from coastal waters off Mar del Plata, Argentina. This species is distinguished from all others in the genus by a rostellar armature consisting of 2 apical groups of 31 large hooks each, arranged in 2 rows (16 anterior, 15 posterior) with 13 hooklets per side, a wide corona of 8-11 rows of spines posterior to the rostellum, 8 rows of 24-26 spines on the cephalic peduncle, and 11-15 testes per proglottid. The new species most closely resembles 2 congeners that also parasitize sharks, Echinobothrium musteli and Echinobothrium scoliodoni, on the basis of the armature of the rostellum and cephalic peduncle and the presence of a corona of small spines. Echinobothrium notoguidoi can be differentiated from E. musteli by the number of hooklets (3) and testes (22), and from E. scoliodoni by the number of large hooks (10-13), spines on the cephalic peduncle (> 100), and segments (40-50). Echinobothrium notoguidoi is clearly distinguished from Echinobothrium pigmentatum described previously from Zapteryx brevirostris in Argentine waters by the following combination of characters: corona of hooks lacking, possession of fewer apical hooks (20), hooklets in a continuous row instead of separate groups, fewer spines in the cephalic peduncle (9-13 per row), and fewer testes (5-7) per proglottid.

  20. Rio Blanco massive hydraulic fracture

    Energy Technology Data Exchange (ETDEWEB)

    1973-01-01

    The Piceance Basin in Colorado contains an estimated 600 trillion cu ft of natural gas in place. Both the Rulison and Rio Blanco events have been detonated to determine the feasibility of nuclear fracturing to stimulate natural gas production in this basin. A demonstration program to test the relative effectiveness of massive hydraulic fracturing (MHF) to achieve natural gas production stimulation from the same gas reservoir is presented. Details are included on MHF design parameters, including surface and subsurface equipment, pumping requirements, evaluation of fracturing results, and all associated test programs; site characteristics and preparation; proposal for gas utilization program; environmental surveillance and comparative analysis of environmental aspects of MHF and nuclear stimulation; gas delivery estimates; project administration; and costs and scheduling.

  1. Parasitosis en camarones reproductores litopenaeus vannamei

    OpenAIRE

    Carpio, Bertha; Cedeño, Ricardo

    2007-01-01

    Parasitosis en Camarones Reproductores Litopenaeus vannamei En las últimas semanas se han reportado mortalidades en reproductores de camarón superiores a las normalmente registradas en los sistemas de maduración de la Península de Santa Elena.

  2. Coeficientes de utilización digestiva aparente de materia seca, proteína y aminoácidos esenciales de ingredientes terrestres para el camarón del Pacífico Litopenaeus vannamei (Decapoda: Penaeidae)

    OpenAIRE

    Martín Terrazas; Roberto Civera; Lilia Ibarra; Ernesto Goytortúa

    2010-01-01

    Los aminoácidos esenciales y su disponibilidad son importantes en la formulación de alimentos. La digestibilidad de materia seca (DAMS), proteína (DAP) y aminoácidos esenciales (DAAA) fueron determinados (triplicado) para el camarón blanco del Pacífico, Litopenaeus vannamei (15-19g), usando una dieta de referencia con 30% (de cada ingrediente). Los ingredientes evaluados fueron: caseína (CAS), harinas de subproductos avícolas (HSPA) y porcícolas (HSPP), gluten de maíz (GLM) y trigo (GLT), pas...

  3. Environmental impacts associated with Project Rio Blanco

    International Nuclear Information System (INIS)

    Alldredge, A.W.; Whicker, F.W.; Hanson, W.C.

    Project Rio Blanco, an experiment involving deep underground detonation of three 30-kton nuclear explosives designed to stimulate natural gas flow in geologic formations of low permeability, was conducted in western Colorado on 17 May 1973. Environmental impacts associated with this experiment were divided into three categories: radiation, ground motion, and conventional physical activities. Radiation and ground motion are unique to this type experiment while conventional activities would be associated with any type of resource development. The objective of observations made at Rio Blanco was to qualitatively and, where possible, quantitatively ascertain environmental impacts associated with the project. Observations indicated that ground motion and conventional activities appeared to cause the greatest impacts. Ground motion impacts were most severe within 2.4 km of the emplacement well (EW) and were predominantly associated with steep ravine and stream banks and rocky cliffs. Following the detonation, flow and turbidity had increased in a small stream adjacent to the EW. Animals receiving deleterious impacts were those associated with stream banks, cliffs and burrows. No mortality or injury was observed in any large animals. (U.S.)

  4. Azul, blanco, rojo. Homenaje a Lavoisier

    Directory of Open Access Journals (Sweden)

    Delia Ángeles García Liarte

    2011-01-01

    Full Text Available Se presenta una experiencia de Química en la que un líquido incoloro, que se muestra en una botella transparente, se vierte en tres vasos en apariencia vacíos, apareciendo de forma sucesiva los colores azul, blanco y rojo. El líquido incoloro no es otro que amoníaco, y los vasos que parecían no llevar nada en su interior contenían cantidades inapreciables de sulfato de cobre (II 5-hidrato para formar el color azul, nitrato de plomo (II para el color blanco y fenolftaleína para el color rojo. La coincidencia de estos tres colores con los de la bandera de Francia nos servirá de pretexto para un breve comentario sobre la figura de Lavoisier. Posteriormente, el contenido de los tres recipientes se vierte en la que, aparentemente, es la misma botella transparente, con lo cual los colores, tal y como han aparecido, desaparecerán formando una disolución incolora. Además de la descripción del procedimiento, en el artículo se analizan aspectos relacionados con los procesos que se han producido en el transcurso de la experiencia y proporciona algunas orientaciones didácticas.

  5. Rio Blanco massive hydraulic fracture: project definition

    Energy Technology Data Exchange (ETDEWEB)

    1976-01-01

    A recent Federal Power Commission feasibility study assessed the possibility of economically producing gas from three Rocky Mountain basins. These basins have potentially productive horizons 2,000 to 4,000 feet thick containing an estimated total of 600 trillion cubic feet of gas in place. However, the producing sands are of such low permeability and heterogeneity that conventional methods have failed to develop these basins economically. The Natural Gas Technology Task Force, responsible for preparing the referenced feasibility study, determined that, if effective well stimulation methods for these basins can be developed, it might be possible to recover 40 to 50 percent of the gas in place. The Task Force pointed out two possible underground fracturing methods: Nuclear explosive fracturing, and massive hydraulic fracturing. They argued that once technical viability has been demonstrated, and with adequate economic incentives, there should be no reason why one or even both of these approaches could not be employed, thus making a major contribution toward correcting the energy deficiency of the Nation. A joint Government-industry demonstration program has been proposed to test the relative effectiveness of massive hydraulic fracturing of the same formation and producing horizons that were stimulated by the Rio Blanco nuclear project.

  6. Rio Blanco massive hydraulic fracture: project definition

    International Nuclear Information System (INIS)

    1976-01-01

    A recent Federal Power Commission feasibility study assessed the possibility of economically producing gas from three Rocky Mountain basins. These basins have potentially productive horizons 2,000 to 4,000 feet thick containing an estimated total of 600 trillion cubic feet of gas in place. However, the producing sands are of such low permeability and heterogeneity that conventional methods have failed to develop these basins economically. The Natural Gas Technology Task Force, responsible for preparing the referenced feasibility study, determined that, if effective well stimulation methods for these basins can be developed, it might be possible to recover 40 to 50 percent of the gas in place. The Task Force pointed out two possible underground fracturing methods: Nuclear explosive fracturing, and massive hydraulic fracturing. They argued that once technical viability has been demonstrated, and with adequate economic incentives, there should be no reason why one or even both of these approaches could not be employed, thus making a major contribution toward correcting the energy deficiency of the Nation. A joint Government-industry demonstration program has been proposed to test the relative effectiveness of massive hydraulic fracturing of the same formation and producing horizons that were stimulated by the Rio Blanco nuclear project

  7. Caminhos da intersubjetividade: Ferenczi, Bion, Matte-Blanco Paths of intersubjectivity: Ferenczi, Bion, Matte-Blanco

    Directory of Open Access Journals (Sweden)

    Ignácio Gerber

    1999-01-01

    Full Text Available A Intersubjetividade é inerente à concepção freudiana de Psicanálise, mas foi Ferenczi, seu discípulo mais próximo, o pioneiro na investigação das emoções que tomam o analista na presença do analisando. Esse artigo retoma aspectos dessa investigação das próprias emoções em Ferenczi, Bion e Matte-Blanco, discutindo as relações entre a lógica racional consciente e essa outra lógica emocional e contraditória do Inconsciente.Intersubjectivity is pertinent to the freudian concept of Psychoanalysis. Yet, it was Ferenczi, his closest disciple, and the pioneer in the investigations of emotions, which come upon the analyst in the presence of the patient. This paper retakes the aspects of this investigation of Ferenczi, Bion and Matte-Blanco, discussing the relations between the rational logic and this other one which is the emotional and contradictory logic of the unconscious.

  8. Efecto de Vibrio harveyi en la sobre vivencia de larvas de Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Gabriel Aguirre-Guzmán

    2013-01-01

    Full Text Available Los cultivos acuícolas son de gran relevancia en la alimentación humana, pero crean un medio ambiente artificial que promueve el crecimiento de diferentes especies de bacterias. Las especies del género Vibrio son bacterias de la microflora normal de los camarones peneidos y son también agentes patógenos oportunistas que pueden tomar ventaja de éstos cambios ecológicos generados en los cultivos acuícolas causando diferentes enfermedades, sobrevivencias bajas y pérdidas económicas en la producción de camarón. El objetivo de la presente investigación fue determinar la variación en la sensibilidad, de diferentes subestadios larvarios (nauplio, zoea I-III, misis I-III y en el de postlarva 1, del camarón blanco del Pacifico Litopenaeus vannamei al ser expuestos a tres dosis [103, 105, y 107 unidades formadoras de colonias (UFC ml-1] de V. harveyi, mediante infecciones por inmersión (30 min. Esta especie generó una sobrevivencia baja significativa en las larvas (p < 0,05 solamente en las dosis más altas (105 y 107 UFC ml-1, siendo la última dosis la que presentó los valores más bajos de sobrevivencia. Además, se observó que los subestadios larvales y en el de postlarva 1 fueron más resistentes a V. harveyi al aumentar la edad de los mismos. Esta información tiene gran significado para la industria acuícola, ya que les permite generar estrategias que disminuyan los efectos de V. harveyi y que les permita mejorar el crecimiento y sobrevivencia de las larvas y de postlarva 1 del camarón.

  9. Mt. Blanco revisited: Soil-geomorphic implications for the ages of the upper Cenozoic Blanco and Blackwater Draw Formations

    Science.gov (United States)

    Holliday, Vance T.

    1988-06-01

    Mt. Blanco, on the eastern edge of the Southern High Plains of Texas, contains stratigraphic features significant in interpreting the late Cenozoic history of the region and the vertebrate paleontology of the Great Plains; however, the stratigraphic relations are confused in the literature or are unreported. Mt. Blanco is the type locality for the Blanco Formation and the Blanco Local Fauna, which occurs throughout North America and is the type fauna for the Blancan Land Mammal Age in North America. Here also occur exposures of the Blackwater Draw Formation, an extensive (˜120000 km2) eolian sheet that is the surficial cover of the region and contains the 1.4 Ma Guaje Ash and several buried soils. A reexamination of the section shows that (1) the Blackwater Draw Formation, an eolian deposit, contains three well-expressed buried soils (5 YR hues, argillic horizons ≥1 m thick, Stages III and IV calcic horizons) and the similar regional surface soil (Paleustalf); (2) the Guaje Ash is within the lower Blackwater Draw Formation but is separated from the Blanco Formation, a lacustrine unit, by about 1 m of sediment, including the lowest buried soil; and (3) the lowest buried soil shows a Stage IV calcrete formed at the top of the Blanco Formation and the base of the Black-water Draw Formation and probably took about 200 ka to form. These new data suggest that deposition of the type Blanco sediments may have ended by about 1.6 Ma or earlier. Since that time, the Blackwater Draw Formation has accumulated episodically; periods of nondeposition are characterized by landscape stability and pedogenesis.

  10. Sensory quality of frozen shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Inês Maria Barbosa Nunes Queiroga

    2014-09-01

    Full Text Available The objective of this research was to evaluate the sensory quality of the marine shrimp Litopenaeus vannamei grown and stored in a freezer. A sensory analysis consisted of the Quality Index Method (MIQ to review the raw shrimp and Descriptive Analysis (AD in cooked shrimp in samples stored for a period of 90 days, using eight previously trained panelists. Accommodation comprising shrimp filet (100-120 pieces / kg samples were subjected to freezing in liquid nitrogen (- 86 °C, Freezing Tunnel (- 35 °C and Domestic Freezer (- 18 °C. At 0, 30, 60 and 90 days of storage in these freezing systems, determination of pH, water holding capacity (WHC, weight loss during cooking (WLC, shearing force, color, total volatile bases (TVB and thiobarbituric acid reactive substances (TBARS were performed. The attributes manual firmness and softness showed better results in rapid freezing. The color parameters (a *, b * and L *, WHC and WCL were higher at 90 days of storage, no significant losses were observed. Highlighted the strength of greater shear in slowly frozen samples at 90 days, confirming the results reported by the sensory panel. At 90 days of storage, the frozen shrimp showed good sensory quality and physical and chemistry characteristics. The shelf life of this shrimp could be set at about 90 days.

  11. Blanco sobre blanco : la arquitectura y el cambio cromático

    Directory of Open Access Journals (Sweden)

    Jesús Marina Barba

    2006-01-01

    Full Text Available El color tiene un impacto considerable en la percepción general del paisaje urbano. La luz resalta las superficies, la textura subraya los materiales. Cuando en un paisaje se realiza cualquier intervención, ésta se convierte en atracción visual en relación a su contexto, siendo necesario considerar los factores determinantes de esta nueva relación. El empleo del blanco brillante supone introducir un elemento perceptivo fuerte. Paradójicamente, es el color más agresivo. Los materiales naturales, como la propia naturaleza, tienden a ser complejos en su textura y en sus tonos. El uso generalizado de productos industriales está conduciendo a un empobrecimiento cromático de los paisajes urbanos. El futuro del color blanco pasa por un cambio en su concepción de elemento decorativo hasta convertirlo en fundamento del proyecto, aprovechando sus cualidades de gradación tonal, contraste, expansión espacial y composición de escalas y formas.The colour has a significant impact on the overall perception of an urban environment. Light plays on surfaces, the texture of materials are noticed. Since any object introduced into the environment becomes a visual target in relation to its context, it is necessary to considerer the factors determining that relationship. Brilliant white forms a visual target that attracts the eye. Paradoxically, is the most intrusive colour. Natural materials, like nature itself, tend to be richly textured and subtly coloured. Widely use of industrial products is leading to a chromatic impoverishment on the urbanscape. The future of white is going through a change in its conception as decorative element, until it is turned to project foundation thanks to its qualities of tonal gradation, contrast, spatial expansion and composition of scales and forms.

  12. Color/No-Color: los blancos en la cultura occidental

    OpenAIRE

    Aoiz Orduna, Javier

    2016-01-01

    Varese Wall, 1975, obra de Robert Ryman que exhibe la Dia Art Foundation de Nueva York, nos sirve de excusa para iniciar nuestro estudio sobre el blanco y su significado en nuestra cultura. La uniformidad y gran tamaño de este monocromo hacen que nos planteemos una serie de interrogantes que conectan la modernidad y su afán de tabula rasa con la búsqueda de belleza y armonía de la Antigüedad clásica. Indagamos sobre la simbología del blanco, su influencia en el arte y en nuestra vida cotidian...

  13. Estrutura populacional de Aegla schmitti (Crustacea: Anomura: Aeglidae nos reservatórios dos Mananciais da Serra, Piraquara, Paraná, Brasil Population structure of Aegla schmitti (Crustacea: Anomura: Aeglidae in the reservoirs of Mananciais da Serra, Piraquara city, state of Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Érico A. O. Teodósio

    2009-03-01

    Full Text Available A study on population structure of Aegla schmitti Hobbs III, 1979 was carried out at Mananciais da Serra, in the municipality of Piraquara, state of Paraná, Brazil (25º29'S, 48º58'W. The following aspects were treated: temporal fluctuation in abundance, sex ratio, reproductive period and size composition based on carapace length. The animals were colleted with traps, nets, measured and returned to the reservoirs. This population was sampled monthly, in two reservoirs (Carvalho and Carvalhinho constructed in the headwater streams with swift current and steep gradient, located on west side of Serra do Mar mountains, from November, 2004 to January, 2006. The water temperature varied from 11.0 (July, 2005 to 18.2ºC (March, 2007, dissolved oxygen from 7.0 to 9.5 mg/l and pH from 4.8 to 6.7, during collection period. A total of 1230 individuals were collected, among which, 819 were males and 411 females (30 ovigerous. The abundance varied from 23 (December, 2004 to 122 (June, 2005 crabs and the sex ratio of the whole population was 1:0.5 (M:F. Male carapace length varied from 4.38 to 36.46 mm and female, from 4.28 to 32.56 mm. Ovigerous females occurred from April, 2005 to November, 2005. Higher crab abundance was recorded in the Carvalho Reservoir, which explained by the presence of numerous breaches on the old tank walls that acted as shelters for these animals. Aegla schmitti females incubate egg mass during colder months and its juveniles hatch mainly in spring, as most Aegla Leach, 1820 species occurring in southeastern and southern Brazil.

  14. Comparison of some reproductive characteristics of farmed and wild white shrimp males Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Sergio Rendón Rodríguez

    2007-03-01

    Full Text Available We rated some reproductive characteristics of white shrimp Litopenaeus vannamei (Boone, 1931 males using 46 farmed individuals (weighing 21.42±0.56 g and 40 wild individuals (weighing 36.10±0.72 g. In farmed shrimps, spermatophore mean weight was 8.94±0.51 mg; total mean sperm count was 3.90±0.27 x 10(6 in each spermatophore; and mean percentage of normal sperm was 86.9±0.37 %. In wild individuals, the respective values were 30.68±2.32 mg; 6.22±1.09 x 10(6; and 62.1±3.56 %. In both groups, the differences between right and left spermatophore were not significant (pResumen Se evaluaron características reproductivas como peso del espermatóforo, número de espermatozoides y porcentaje de espermatozoides normales en 86 organismos de camarón blanco (Litopenaeus vannamei. de éstos, 46 individuos provinieron de estanques de cultivo con un peso de 21.42±0.56 g y el resto, 40, silvestres con un peso de 36.10±0.72 g. En los camarones cultivados, el peso de los espermatóforos fue de 8.94±0.51 mg; el número total de espermatozoides en cada espermatóforo fue de 3.90±0.27 x 10(6; y el porcentaje de espermatozoides normales fue de 86.9±0.37 %. En los silvestres, los valores respectivos fueron de 30.68±2.32 mg; 6.22±1.09 x 10(6; y 62.1±3.56 %. En ambos grupos, las diferencias entre los espermatóforos derecho e izquierdo no fueron estadísticamente diferentes (p<0.01. Las diferencias en el peso de los espermatóforos y el porcentaje de espermatozoides normales, entre los camarones cultivados y silvestres, fueron significativas (p<0.01; sin embargo, en el número de espermatozoides tales diferencias no fueron significativas (p<0.01. La relación entre el peso del espermatóforo (Ws y el peso de los individuos (Wo fue Ws (mg=1.23(Wo-17.34 (r²=0.89, en camarones cultivados; y Ws (mg=2.57(Wo-60.04 (r²=0.64, en los silvestres. En organismos cultivados, la relación entre el número de espermatozoides (Cs y el peso de los individuos (Wo fue

  15. Modification of the colony tower for the Rio Blanco detonation

    International Nuclear Information System (INIS)

    Blume, J.A.; Freeman, S.A.; Honda, K.K.; Lee, L.A.

    1975-01-01

    Supplemental structural bracing was designed and installed for the 180-ft-tall Colony Tower, an experimental oil shale processing retort structure, in anticipation of its lateral response to the Rio Blanco detonation. The tower is a steel structure with both horizontal and vertical diagonal bracing. Data obtained from the earlier Project Rulison detonation indicated that an evaluation study was necessary. Design criteria that would provide an adequate margin of safety were developed based on predicted Rio Blanco ground motion. The evaluation of the unmodified structure showed that several bracing members would be subjected to forces exceeding their yield strength, and some would reach a level at which failure could occur. Further analyses were made with assumed modified bracing members. A final scheme for modified vertical bracing was established and installed. After modification, the response of the tower during the Rio Blanco detonation was measured by instruments on the ground and at various locations on the tower, and no evidence of damage was discovered. The modification of the Colony Tower and the procedures used to determine these modifications show the usefulness of current ground motion and structural response prediction technology for forecasting dynamic behavior of important structures subjected to ground motion from underground nuclear explosions. (auth)

  16. Los sindicatos blancos de Monterrey (1931-2009)

    OpenAIRE

    Miguel Ángel Ramírez Sánchez

    2011-01-01

    Los sindicatos blancos son el equivalente mexicano a los sindicatos amarillos de Francia y España y a los planes de representación del empleado de Estados Unidos y Canadá. De ellos se dice que son organismos obreros sometidos a la voluntad de los patrones, que los crean y dirigen. En México, esta clase de sindicatos eran hasta hace poco un fenómeno regional, restringido a la ciudad de Monterrey, donde han tenido su mayor desarrollo. Sin embargo, coincidiendo con la crisis y reorganización del...

  17. Fiebre del oro blanco: la cocaína

    Directory of Open Access Journals (Sweden)

    Iván Marín Argüello

    1999-12-01

    Full Text Available La planta de coca, Erythroxylon coca es cultivada en la zona occidental de América del Sur exigiendo condiciones de suelo muy particulares. La mezcla de las hojas de cocaína con sustancias químicas origina un polvo cristalino blanco, conocido comúnmente como cocaína. Al producto obtenido del tratamiento de la cocaína con solventes se le denomina crack. El consumo de cocaína, las formas de administración más diversas y el estado fisiológico logrado por los usuarios, es denominado flash 0 high Los efectos neuro-fisiológicos son muy variados, provocando sobre todo un fuerte estímulo al Sistema Nervioso Central (SNC, producto de la liberación de dopamina en las terminaciones nerviosas.

  18. Los sindicatos blancos de Monterrey (1931-2009

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Ramírez Sánchez

    2011-01-01

    Full Text Available Los sindicatos blancos son el equivalente mexicano a los sindicatos amarillos de Francia y España y a los planes de representación del empleado de Estados Unidos y Canadá. De ellos se dice que son organismos obreros sometidos a la voluntad de los patrones, que los crean y dirigen. En México, esta clase de sindicatos eran hasta hace poco un fenómeno regional, restringido a la ciudad de Monterrey, donde han tenido su mayor desarrollo. Sin embargo, coincidiendo con la crisis y reorganización del sindicalismo mexicano y la apertura del sistema político nacional, han crecido en número e influencia política. En este artículo se ofrece un resumen histórico de este movimiento.

  19. Cantharellus gallaecicus (Blanco-Dios Olariaga, comb. & stat. nov (Cantharellaceae

    Directory of Open Access Journals (Sweden)

    Olariaga, Ibai

    2007-12-01

    Full Text Available Cantharellus gallaecicus comb. & stat. nov. is proposed, after the examination of its holotype and additional material. Based on the characters observed in all the studied material (i.e., thinwalled hyphae of the pileipelis, minute basidiomata with white to grey pileus, and surface that turns yellow when bruised it is considered that C. gallaecicus is more closely related to C. romagnesianus than to C. cibarius.Se propone Cantharellus gallaecicus comb. & stat. nov. tras revisar su holótipo y material adicional disponible. Dado que todo el material examinado posee hifas del pileipelis de pared delgada, basidiomas pequeños con píleo de blanco a gris, y superficie que vira a amarillo al roce, se considera que C. gallaecicus es una especie más estrechamente relacionada con C. romagnesianus que con C. cibarius.

  20. DURABILIDAD DEL CEMENTO PORTLAND BLANCO ADICIONADO CON PIGMENTO AZUL ULTRAMAR

    Directory of Open Access Journals (Sweden)

    CAROLINA GIRALDO

    2010-01-01

    Full Text Available El pigmento Azul Ultramar (AU es un aluminosilicato polisulfurado de sodio que reacciona con el aluminato tricálcico (C3A y con el óxido de calcio (CaO del cemento Pórtland blanco en presencia de agua, generando cantidades considerables de etringita a edad temprana y en menor proporción de tobermorita. Esta etringita primaria se presenta en forma de fibras no orientadas mejorando el desempeño mecánico de los morteros, y al mismo tiempo dejando pocas cantidades de C3A disponible para la formación de etringita secundaria. En esta investigación se evalúa la durabilidad a diferentes edades de curado en morteros de cemento Portland blanco sustituidos por 0%, 10% y 20% de AU en peso, mediante pruebas de succión capilar y evaluación del cambio longitudinal de morteros expuestos a una solución de sulfato de sodio con una concentración del 5% (ASTM C1012. Los resultados evidencian una mayor resistencia a compresión y a flexión, una significativa disminución de la expansión y una reducción hasta del 800% de la absorción de agua en morteros con AU. Todo esto debido a la formación de las fases minerales adicionales (etringita primaria y tobermorita, las cuales fueron identificadas mediante microscopía electrónica de barrido (SEM.

  1. Humoral and Haemocytic Responses of Litopenaeus vannamei to Cd Exposure

    Science.gov (United States)

    Bautista-Covarrubias, Juan C.; Velarde-Montes, Germán J.; García-de la Parra, Luz M.; Soto-Jiménez, Martín F.; Frías-Espericueta, Martín G.

    2014-01-01

    White shrimp, Litopenaeus vannamei, subadults were exposed to four dilutions of the 96 h cadmium LC50 reported for postlarvae (PL12) of this species, and the effects were evaluated after 5, 48, and 96 h of exposure. While treatments did not affect survival and hemolymph clotting time increased with time, but not as a response to Cd exposure, the intensity of other responses was related to concentration, to time of exposure, and to their interaction. Hemocyanin decreased with time in all metal concentrations but increased in the control treatment, and an almost similar trend was observed with hemocyte numbers. As an initial response, phenoloxidase activity decreased with all metal concentrations, but it increased later to values similar or higher than the control treatment. PMID:24967441

  2. Humoral and Haemocytic Responses of Litopenaeus vannamei to Cd Exposure

    Directory of Open Access Journals (Sweden)

    Juan C. Bautista-Covarrubias

    2014-01-01

    Full Text Available White shrimp, Litopenaeus vannamei, subadults were exposed to four dilutions of the 96 h cadmium LC50 reported for postlarvae (PL12 of this species, and the effects were evaluated after 5, 48, and 96 h of exposure. While treatments did not affect survival and hemolymph clotting time increased with time, but not as a response to Cd exposure, the intensity of other responses was related to concentration, to time of exposure, and to their interaction. Hemocyanin decreased with time in all metal concentrations but increased in the control treatment, and an almost similar trend was observed with hemocyte numbers. As an initial response, phenoloxidase activity decreased with all metal concentrations, but it increased later to values similar or higher than the control treatment.

  3. Isolation and characterization of infectious Vibrio sinaloensis strainsfrom the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Ma. del Carmen Flores-Miranda

    2012-06-01

    Full Text Available Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9±1.1g, per tank. The treatments in triplicate were: (1 saline solution (control group; (2 2×10(5CFU/shrimp; (3 4×10(5CFU/shrimp; (4 2×10(6CFU/shrimp; (5 4×10(6CFU/shrimp, and (6 8×10(6CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×10(5CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei. Rev. Biol. Trop. 60 (2: 567-576. Epub 2012 June 01.Las enfermedades de etiología infecciosa, especialmente las causadas por patógenos bacterianos y virales ocasionan graves pérdidas en el cultivo de camarón blanco Litopenaeus vannamei. En este estudio se caracterizo: la actividad enzimática y hemolítica; hidrofobicidad; crecimiento e identificación molecular de vibrios aislados del intestino y hepatopancreas de camarones estresados, obtenidos de una granja comercial, en medio Agar Tiosulfato Citrato Bilis Sacarosa. Además, se realizaron tres infecciones experimentales para confirmar la patogenicidad de las cepas bacterianas

  4. El destierro infinito de Blanco White en la mirada de Juan Goytisolo

    Directory of Open Access Journals (Sweden)

    Durán López, Fernando

    2010-06-01

    Full Text Available In the years 1970-1972, the novelist Juan Goytisolo published several studies and translations on the work of José María Blanco White, which represent a key moment in the recovery of that forgotten writer for the modern Spanish culture. The argument made in this essay is that the consciously identifying strategy applied by Goytisolo on Blanco White, in order to define a Spanish intellectual tradition throughout the centuries, marked by exile and dissent, is in fact a deformation of the figure and thought of the writer from Seville. Blanco White’s reception in Spain, however, has been conditioned by that reading.En torno a los años 1970-1972 el novelista Juan Goytisolo publica varios estudios y traducciones sobre la obra de José María Blanco White, que suponen un momento clave en la recuperación de ese olvidado escritor para la moderna cultura española. La tesis formulada en el presente artículo es que la estrategia conscientemente identificadora que aplica Goytisolo sobre Blanco White, a fin de definir una tradición intelectual española a lo largo de los siglos marcada por el exilio y la disidencia, supone de hecho una deformación de la figura y el pensamiento del escritor sevillano. La recepción de Blanco White en España ha quedado, sin embargo, condicionada por esa lectura.

  5. Nevus blanco esponjoso familiar Family nevus spongiosus albus

    Directory of Open Access Journals (Sweden)

    Mônica Andrade Lotufo

    2010-06-01

    Full Text Available El nevus blanco esponjoso (NBE es una rara condición autosómica dominante, caracterizada por placas blancas bilaterales en la mucosa, de aspecto esponjoso, blandas a la palpación y que pueden escamarse. Los tratamientos son paliativos; y el uso de antibióticos, en especial la tetraciclina, ha demostrando buenos resultados en su control. Este trabajo presenta tres casos clínicos de una familia afectada por NBE, donde se discuten los posibles diagnósticos diferenciales y conductas terapéuticas indicadas. Un paciente masculino de 52 años de edad acudió a la clínica aquejado de lesiones blancas bilaterales. El paciente notó las lesiones 30 años antes, sin lograr un diagnóstico final de las mismas. Después de la anamnesis y del examen clínico fue realizada una biopsia incisional. La reunión de los datos clínicos e histopatológicos llevó al diagnóstico de NBE. Se le solicitó al paciente que indagase entre sus familiares con respecto a lesiones semejantes. Se detectó que el hijo de 19 años y la hija de 25 eran portadores de placas blancas en la mucosa yugal. Como no había afectación estética, se optó por no intervenir en las lesiones. El nevus blanco esponjoso es una lesión genética que debe ser diferenciada de otras patologías localizadas y sistémicas importantes, que tienen repercusiones serias para el individuo. Como no hay un tratamiento curativo para el NBE, el papel del cirujano dentista es diagnosticar esta lesión, aclarar al paciente sobre la naturaleza benigna y autolimitante del NBE y si fuera necesario desde el punto de vista estético, aplicar diferentes modalidades terapéuticas.The aim of present paper is to introduce three clinical cases from a family affected from nevus spongiosus albus (NSA and also to discuss the possible differential diagnoses as well as the therapeutical behaviors to be adopted. Clinical case: A man aged 52 seen in our clinic due to bilateral white lesions noted 30 years ago without

  6. Coeficientes de utilización digestiva aparente de materia seca, proteína y aminoácidos esenciales de ingredientes terrestres para el camarón del Pacífico Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Martín Terrazas

    2010-12-01

    Full Text Available Los aminoácidos esenciales y su disponibilidad son importantes en la formulación de alimentos. La digestibilidad de materia seca (DAMS, proteína (DAP y aminoácidos esenciales (DAAA fueron determinados (triplicado para el camarón blanco del Pacífico, Litopenaeus vannamei (15-19g, usando una dieta de referencia con 30% (de cada ingrediente. Los ingredientes evaluados fueron: caseína (CAS, harinas de subproductos avícolas (HSPA y porcícolas (HSPP, gluten de maíz (GLM y trigo (GLT, pasta de soya (PS, harinas de sorgo (HS y trigo (HT. La DAMS y DAP variaron entre 68%-109% y 70%-103%, respectivamente. La DAP en CAS, PS, HT y GLT fue mayor al 90%, en GLM y HSPA superior a 80%; HSPP (76% y HS (70% tuvieron menor digestibilidad. Hubo concordancia entre DAP y DAAA, excepto para Arg en GLM, Fen y Leu en HS, Fen en PS y Lis en HT y HSPA. Se encontró una gran variabilidad en la DAMS, DAP y DAAA en los ingredientes, lo que debe ser tomado en cuenta al formular alimentos para camarón.

  7. Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae)

    Science.gov (United States)

    Santiago, Librado A; Mayor, Anna Beatriz R

    2014-01-01

    Objective To assess the antioxidant activity of Ficus pseudopalma Blanco (Moraceae) (F. pseudopalma) and characterize the active components present in it. Methods Column chromatography of crude ethanol leaf extract of F. pseudopalma was performed and seven fractions were obtained, labeled as F1, F2, F3, F4, F5, F6, F7. DPPH, FRAP, Griess, Fenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions. Thin layer chromatography (TLC), high performance liquid chromatography and Fourier transfer infrared spectroscopy (FTIR) were performed to identify and characterize the bioactive component present in each fractions of F. pseudopalma. Results DPPH and FRAP assay showed that F5, F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions. All fractions exhibited a good nitric oxide radical scavenging activity wherein F1, F2 and F3 showed the highest inhibition. However, all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals. Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions. Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6. Infrared spectra of the fractions revealed the presence of C-C, OH, aromatic C=C and C=O groups. Conclusions The identified lupeol in F. pseudopalma may be responsible for the exhibited antioxidant property of the plant. Furthermore, knowing the antioxidant capability of the plant, F. pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases. PMID:25182281

  8. The effects of boiling on the allergenic properties of tropomyosin of shrimp (litopenaeus vannamei).

    Science.gov (United States)

    Shrimp play an important role in human nutrition, and is responsible for severe hypersensitivity reactions. The thermal stability of raw and boiled shrimp tropomyosins (TM) has never been reported. The aims of the study were to compare the stability of raw and boiled shrimp TM of Litopenaeus vanname...

  9. Enfermedades en camarones penaeidos: microsporidiosis en penaeus (litopenaeus) vannamei (parte i)

    OpenAIRE

    Carpio, Bertha

    2007-01-01

    ENFERMEDADES EN CAMARONES PENAEIDOS: Microsporidiosis en Penaeus (Litopenaeus) vannamei (Parte I) El virus de la “mancha blanca” ha sido sin duda la enfermedad mas severa que ha sufrido la industria camaronera del Ecuador en los últimos 20 años de producción.

  10. Relation on phitoplankton community with Litopenaeus vannamei productivity in biocrete pond

    Directory of Open Access Journals (Sweden)

    T. Budiardi

    2007-07-01

    Full Text Available ABSTRACTManagement of phytoplankton is generally conducted by controlling the concentration of organic matter, fertilization and water exchange.  Organic materials are from uneaten feed and excretion of shrimp.  By using post facto method it was found four class of phytoplankton in biocrete pond at one cycle rearing of white shrimp (Litopenaeus vannamei.  Population at early rearing period was dominated by Bacillariophyceae (50.4%; 13 species and Cyanophyceae (42.41%; 1 species, followed by Dynophyceae (6.2%; 5 species and Chlorophyceae (1.3%; 1 species.  Increment in phytoplankton density was followed by increment in chlorophyll-a and oxygen from photosynthesis, and productivity was 2132 kg/pond.Keywords: phytoplankton, white shrimp, Litopenaeus vannamei, biocrete pond ABSTRAKPengelolaan fitoplankton umumnya dilakukan dengan mengoptimalkan bahan organik serta pemupukan dan pergantian air. Bahan organik berasal dari pakan buatan yang tidak terkonsumsi (sisa pakan dan ekskresi dari udang. Dengan menggunakan metode post facto selama satu siklus pemeliharaan udang vaname (Litopenaeus vannamei pada tambak biocrete diperoleh empat kelas fitoplankton. Dominasi Bacillariophyceae (50,4%; 13 jenis, Cyanophyceae (42,41%; 1 jenis terjadi pada awal pemeliharaan yang diiukuti oleh Dynophyceae (6,2%; 5 jenis dan Chlorophyceae (1,3%; 1 jenis. Peningkatan kelimpahan fitoplankton secara keseluruhan diikuti oleh peningkatan kandungan klorofil-a dan oksigen hasil fotosintesis total sehingga produktifitasnya mencapai 2132 kg/petakKata kunci: fitoplankton, udang vaname, Litopenaeus vannamei, tambak biocrete

  11. Characterization of vitellin from the ovaries of the banana shrimp Litopenaeus merguiensis.

    Science.gov (United States)

    Auttarat, Jongruk; Phiriyangkul, Pharima; Utarabhand, Prapaporn

    2006-01-01

    Vitellin (Vt) was purified from ovary extracts of mature females of the banana shrimp Litopenaeus merguiensis using DEAE-Sephacel and Superdex 200 columns. Native Vt had an apparent molecular mass of 398 kDa as determined by native PAGE and by gel filtration chromatography. Under reducing and denaturing conditions (SDS-PAGE), Vt is composed of two major subunits of 87 and 78 kDa, although some faint bands were also detected. The N-terminal 10 amino acids sequence of the 78 kDa subunit is identical to that of Litopenaeus vannamei Vt and very similar to that of Litopenaeus japonicus vitellogenin (Vg) as well as Litopenaeus semisulcatus Vt, with an identity of 89%. Anti-Vt polyclonal antibody raised against purified Vt shows a high specificity with only ovarian Vt and hemolymph Vg of vitellogenic shrimps in double immunodiffusion and Western blot assays. Vg and Vt concentrations in hemolymph, hepatopancreas and ovaries were measured by ELISA. Vg concentrations increased in the hemolymph in the early stages of ovarian development and declined in the maturation stages. As there were undetectable concentrations of Vg in the hepatopancreas while an elevation of Vg levels occurred in the hemolymph, during the time that Vt was accumulating in the ovaries during oogenesis, this would suggest that the contribution of Vg synthesized by the hepatopancreas only might be not sufficient for adequate development of the oocytes in the banana shrimp L. merguiensis during vitellogenesis.

  12. ZOOARQUEOLOGÍA DE LA ALDEA FORMATIVA DE PALO BLANCO (TINOGASTA, CATAMARCA, ARGENTINA / Zooarchaeology of the formative village of Palo Blanco (Tinogasta, Catamarca, Argentina

    Directory of Open Access Journals (Sweden)

    Juan Pablo Miyano

    2015-12-01

    Full Text Available En el presente trabajo se abordan los conjuntos arqueofaunísticos de la localidad arqueológica de Palo Blanco recuperados en intervenciones realizadas en la década de 1960 y en la década de 2000. Esta localidad se encuentra emplazada en el sector norte del bolsón de Fiambalá (Tinogasta, Catamarca y se compone de varios núcleos habitacionales de distribución dispersa, predominantemente rectangulares y con muros de tapia. Su ocupación no fue sincrónica y se extiende desde el siglo III al siglo X A.D. La tendencia en el aprovechamiento de recursos faunísticos identificada fundamentalmente a partir del abordaje de los conjuntos de dos núcleos habitacionales sugiere que los camélidos fueron el principal recurso de origen animal utilizado en la aldea, en donde se encontraron especies tanto domesticadas (Lama glama como silvestres (Vicugna vicugna. Además, la presencia predominante de camélidos subadultos sugiere una estrategia de pastoreo vinculada al aprovechamiento de recursos primarios como carne, médula y grasa. Los resultados a los que se arribaron permiten profundizar en el conocimiento de las actividades económicas de los grupos humanos que habitaron la aldea de Palo Blanco a lo largo del primer milenio de la era. AbstractIn this paper we approach the archaeofaunistic remains recovered during excavations of the archeological locality of Palo Blanco in the decades of 1960 and 2000. Palo Blanco is located in the north sector of the Bolsón of Fimabalá area (Tinogasta, Catamarca and it is composed of several rectangular shaped dwellings with tapia-style walls and dispersed distribution. The period of occupation of the village was between the third and tenth century A.D. although the dwellings were not inhabited synchronously. The identified tendency in the exploitation of faunal resources indicates that the camelids were the main resources. Osteometric studies show that domestic and wild camelids (Lama glama and Vicugna vicugna

  13. Rio Blanco gas composition: preproduction testing of the RBE-01 wellhead

    International Nuclear Information System (INIS)

    Smith, C.F.; Fontanilla, J.E.

    1976-01-01

    The chemical composition and radionuclide concentration of Rio Blanco gas samples collected prior to the production testing of the RBE-01 well and analyzed at LLL are presented. The analytical procedures and their uncertainties are briefly summarized. Information that associates the analytical data with the field operations is included

  14. Estudio sobre el urbanismo moderno: Adolfo Blanco y Pérez del Camino, pensionado por la JAE en 1927

    Directory of Open Access Journals (Sweden)

    Díez Ibargoitia, María

    2013-03-01

    Full Text Available Adolfo Blanco carried out one of the first studies within the discipline of urban planning. The work analyzed includes 66 plates (plans and drawings for the expansion of cities in North Africa and Europe.Adolfo Blanco realizó uno de los primeros estudios dentro de la disciplina urbanística. La obra analizada contiene 66 láminas (entre planos y dibujos de los ensanches de las ciudades del Norte de África y Europa.

  15. Project Rio Blanco definition plan. Additional formation evaluation and production testing

    International Nuclear Information System (INIS)

    1975-09-01

    Since the multiple Rio Blanco detonation three reentry wells have been drilled for test purposes: RB-E-01 (Emplacement Well); RB-AR-2 (Alternate Reentry Well); and RB-U-4 (Formation Evaluation Well). Additional testing in all these wells is now required to resolve some remaining technical questions. A plan describing the procedures, methods, responsibilities, and scheduling of the field operations is presented

  16. Evaluation of phytochemical and pharmacological properties of Aegiceras corniculatum Blanco (Myrsinaceae) bark

    OpenAIRE

    Bose, Utpal; Bala, Vaskor; Rahman, Ahmed A.; Shahid, Israt Z.

    2010-01-01

    The methanol extract of the dried barks of Aegiceras corniculatum Blanco (Myrsinaceae) was investigated for its possible antinociceptive, cytotoxic and antidiarrhoeal activities in animal models. The preliminary studies of A. corniculatum bark showed the presence of alkaloids, glycosides, steroids, flavonoids, saponins and tannins. The extract produced significant writhing inhibition in acetic acid-induced writhing in mice at the oral dose of 250 and 500 mg/kg body weight (P < 0.001) comp...

  17. Coeficientes de utilización digestiva aparente de materia seca, proteína y aminoácidos esenciales de ingredientes terrestres para el camarón del Pacífico Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Martín Terrazas

    2010-12-01

    Full Text Available Los aminoácidos esenciales y su disponibilidad son importantes en la formulación de alimentos. La digestibilidad de materia seca (DAMS, proteína (DAP y aminoácidos esenciales (DAAA fueron determinados (triplicado para el camarón blanco del Pacífico, Litopenaeus vannamei (15-19g, usando una dieta de referencia con 30% (de cada ingrediente. Los ingredientes evaluados fueron: caseína (CAS, harinas de subproductos avícolas (HSPA y porcícolas (HSPP, gluten de maíz (GLM y trigo (GLT, pasta de soya (PS, harinas de sorgo (HS y trigo (HT. La DAMS y DAP variaron entre 68%-109% y 70%-103%, respectivamente. La DAP en CAS, PS, HT y GLT fue mayor al 90%, en GLM y HSPA superior a 80%; HSPP (76% y HS (70% tuvieron menor digestibilidad. Hubo concordancia entre DAP y DAAA, excepto para Arg en GLM, Fen y Leu en HS, Fen en PS y Lis en HT y HSPA. Se encontró una gran variabilidad en la DAMS, DAP y DAAA en los ingredientes, lo que debe ser tomado en cuenta al formular alimentos para camarón.Apparent digestion coefficients for dry matter, protein and essential amino acids in terrestrial ingredients for Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae. Protein quality mainly depends on the essential amino acid (EAA profile, but also on its bioavailability, because EAA digestibility is generally lower than the analyzed amounts. This information is needed in the aquaculture industry for aquafeed formulation. For this purpose, the apparent digestibility coefficients of dry matter, protein, and essential amino acids of eight feedstuffs of terrestrial origin were determined for the juvenile whiteleg shrimp Litopenaeus vannamei (15-19g, using 1% chromic oxide as an inert marker. A reference diet was formulated and produced in the laboratory. Eight experimental diets were prepared each with 30% of one of the experimental ingredients added to the reference diet: casein, porcine byproduct meal poultry byproduct meal, corn meal, wheat gluten meal, soybean

  18. Chemical composition, antioxidant and antimicrobial activities of citrus jambhiri lush and citrus reticulata blanco essential oils

    International Nuclear Information System (INIS)

    Sadaf, S.; Shahid, M.; Iqbal, Z.

    2009-01-01

    The aim of this study was to investigate the time interval in which we can get maximum concentration of essential oil from the peels of Citrus jambhiri Lush and Citrus reticulata Blanco, to determine the composition of peel oils and to evaluate the antioxidant and antimicrobial activity of extracted oils. It was observed that in case of Citrus jambhiri Lush maximum oil yield (I %) was obtained when fruits were immature (during October). As the fruit samples got matured, the oil yield decreased. In December the oil yield decreased to 0.2 %. In case of Citrus reticulata Blanco maximum oil yield (0.189 %) was obtained during the last week of January. Chemical analysis of essential oils showed that limonene was the most abundant compound (86 %-93 %) followed by alpha terpinene (2 %-4.5 %), beta-pinene(1 0/0-2 %) and nerol (0.5 %-1.5 %). The radical scavenging and antioxidant activities of essential oils were determined by DPPH and linoleic acid test. The essential oil of Citrus jambhiri Lush inhibited the oxidation of linoleic acid by 54.98 % and that of Citrus reticulata Blanco inhibited by 49.98 %. Moreover, the essential oils also showed antimicrobial activities against the tested microorganisms. (author)

  19. 2015 Long-Term Hydrologic Monitoring Program Sampling and Analysis Results at Rio Blanco, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Findlay, Rick [Nararro Research and Engineering, Oak Ridge, TN (United States); Kautsky, Mark [US Department of Energy, Washington, DC (United States). Office of Legacy Management

    2015-12-01

    The U.S. Department of Energy (DOE) Office of Legacy Management conducted annual sampling at the Rio Blanco, Colorado, Site for the Long-Term Hydrologic Monitoring Program (LTHMP) on May 20–21, 2015. This report documents the analytical results of the Rio Blanco annual monitoring event, the trip report, and the data validation package. The groundwater and surface water monitoring samples were shipped to the GEL Group Inc. laboratories for conventional analysis of tritium and analysis of gamma-emitting radionuclides by high-resolution gamma spectrometry. A subset of water samples collected from wells near the Rio Blanco site was also sent to GEL Group Inc. for enriched tritium analysis. All requested analyses were successfully completed. Samples were collected from a total of four onsite wells, including two that are privately owned. Samples were also collected from two additional private wells at nearby locations and from nine surface water locations. Samples were analyzed for gamma-emitting radionuclides by high-resolution gamma spectrometry, and they were analyzed for tritium using the conventional method with a detection limit on the order of 400 picocuries per liter (pCi/L). Four locations (one well and three surface locations) were analyzed using the enriched tritium method, which has a detection limit on the order of 3 pCi/L. The enriched locations included the well at the Brennan Windmill and surface locations at CER-1, CER-4, and Fawn Creek 500 feet upstream.

  20. Geologic framework, hydrostratigraphy, and ichnology of the Blanco, Payton, and Rough Hollow 7.5-minute quadrangles, Blanco, Comal, Hays, and Kendall Counties, Texas

    Science.gov (United States)

    Clark, Allan K.; Golab, James A.; Morris, Robert E.

    2016-09-13

    This report presents the geologic framework, hydro­stratigraphy, and ichnology of the Trinity and Edwards Groups in the Blanco, Payton, and Rough Hollow 7.5-minute quad­rangles in Blanco, Comal, Hays, and Kendall Counties, Texas. Rocks exposed in the study area are of the Lower Cretaceous Trinity Group and lower part of the Fort Terrett Formation of the Lower Cretaceous Edwards Group. The mapped units in the study area are the Hammett Shale, Cow Creek Limestone, Hensell Sand, and Glen Rose Limestone of the Trinity Group and the lower portion of the Fort Terrett Formation of the Edwards Group. The Glen Rose Limestone is composed of the Lower and Upper Members. These Trinity Group rocks con­tain the upper and middle Trinity aquifers. The only remaining outcrops of the Edwards Group are the basal nodular member of the Fort Terrett Formation, which caps several hills in the northern portion of the study area. These rocks were deposited in an open marine to supratidal flats environment. The faulting and fracturing in the study area are part of the Balcones fault zone, an extensional system of faults that generally trends southwest to northeast in south-central Texas.The hydrostratigraphic units of the Edwards and Trinity aquifers were mapped and described using a classification system based on fabric-selective or not-fabric-selective poros­ity types. The only hydrostratigraphic unit of the Edwards aquifer present in the study area is hydrostratigraphic unit VIII. The mapped hydrostratigraphic units of the upper Trinity aquifer are (from top to bottom) the Camp Bullis, upper evaporite, fossiliferous, and lower evaporite which are interval equivalent to the Upper Member of the Glen Rose Limestone. The middle Trinity aquifer encompasses (from top to bottom) the Lower Member of the Glen Rose Limestone, the Hensell Sand Member, and the Cow Creek Limestone Member of the Pearsall Formation. The Lower Member of the Glen Rose Limestone is subdivided into six informal hydro

  1. Effect of some organophosphorus pesticides on oxygen consumption of shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Galindo Reyes, José Guillermo; Dalla-Venezia, Luisella; Lazcano Alvarez, Maria Guadalupe

    2002-06-01

    Oxygen consumption was measured in adult specimens of Litopenaeus vannamei shrimps from a coastal lagoon of Sinaloa, Mexico, during exposure to sublethal concentrations of the organophosphorus pesticides, Diazinon, Folidol, and Gusathion. Each individual was used as a control of itself, to avoid differences between treatments being masked by individual variability. In all three treatments with pesticides, respiration rate, measured by a polarographic electrode, was significantly lower than in controls. This may, at least partly, explain the decrease in shrimp production observed in recent years in the coastal lagoons of Sinaloa. (c) 2002 Elsevier Science (USA).

  2. Influence of probiotics on the growth and digestive enzyme activity of white Pacific shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Gómez, R. Geovanny D.; Shen, M. A.

    2008-05-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were determined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics ( Bacillus spp.) supplemented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly ( Pprobiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly ( Pprobiotic-treated shrimp than in the control.

  3. Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin

    Directory of Open Access Journals (Sweden)

    Fahmi Akbar

    2015-10-01

    Full Text Available ABSTRACT The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could  influence  the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG and antidopamine (AD injection on white shrimp Litopenaeus vannamei sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose  of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of L. vannamei shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm. Keyword: PMSG, AD, sperm quality, Litopenaeus vannamei  ABSTRAK Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan pregnant mare serum gonadotropin (PMSG dan antidopamin (AD terhadap kualitas sperma udang vaname Litopenaeus vannamei. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati

  4. Quality of antimicrobial products used in white leg shrimp (Litopenaeus vannamei) aquaculture in Northern Vietnam

    DEFF Research Database (Denmark)

    Tran, Kim Chi; Tran, Minh Phu; Phan, Thi Van

    2018-01-01

    Antimicrobials are important to treat diseases in aquaculture and the objective of this study was to evaluate the quality of antimicrobial products commonly used in white leg shrimp (Litopenaeus vannamei) aquaculture in Northern Vietnam. A total of 25 antimicrobial products were obtained from 20....... The documented poor quality of antimicrobial products and inadequate labelling has negative impacts on effective disease treatment; contribute to development of antimicrobial resistance, and the use of such products is associated with food safety and occupational health hazards. There is an urgent need...

  5. Comparison of immune response of Pacific white shrimp, Litopenaeus vannamei, after multiple and single infections with WSSV and Vibrio anguillarum

    Science.gov (United States)

    Our previous study demonstrated that Pacific white shrimp (Litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen [1]. For better understanding the defense mechanism against white spot syndrome virus (WSSV...

  6. Sensitivity of Larvae and Adult and the Immunologic Characteristics of Litopenaeus vannamei under the Acute Hypoxia

    Directory of Open Access Journals (Sweden)

    Hailong Zhou

    2014-01-01

    Full Text Available Litopenaeus vannamei is one of the most commercially important species of shrimp in the world. In this study, we performed acute hypoxia tests with Litopenaeus vannamei to estimate 12 h median lethal concentration (LC50 values at different life stages. The results indicated that the 12 h LC50 values were significantly different in different life stages of shrimp (P<0.05. The maximum value of 12 h LC50 was 2.113 mg L−1 for mysis III, and the minimum value was 0.535 mg L−1 for adult shrimp with an average total length of 6 cm. The study also determined the hemocyanin concentration (HC and the total hemocyte counts (THC in the conditions of hypoxia and reoxygenation. These results showed that the THC decreased and the HC increased under hypoxia, and the THC increased and the HC decreased in the condition of reoxygenation. These results can provide fundamental information for shrimp farming and seedling and also can guide the breeding selection, as well as being very helpful to better understand the hypoxia stress mechanism of shrimp.

  7. Effects of cultured shrimp (Litopenaeus vannamei consumption on serum lipoproteins of healthy normolipidemic men

    Directory of Open Access Journals (Sweden)

    Farzaneh Yousefi

    2012-12-01

    Full Text Available Background: It has been suggested that moderate shrimp consumption in normolipidemic subjects will not adversely affect the overall lipoprotein profile. Hence, shrimp consumption can be included in “healthy heart" nutritional guidelines. However, the effects of cultured shrimp on serum lipoproteins of normal subjects have not yet investigated. Material and Methods: Twenty-five healthy normolipidemic men who were workers of a shrimp farm in Bushehr province participated in a quasi-experimental study. In a crossover six weeks trial, the effect of three days per week diet (containing 300 g cultured shrimp Litopenaeus vannamei /day on serum lipid profile was compared with a zero-marine baseline diet. Results: After six weeks trial, serum triglyceride and HDL-cholesterol levels were not significantly changed from the baseline levels (p>0.05. However, total cholesterol and LDL-cholesterol levels, total cholesterol to HDL-cholesterol and LDL-cholesterol to HDL-cholesterol ratios were significantly increased (p<0.0001. Conclusion: Moderate cultured shrimp (Litopenaeus vannamei consumption can increase total cholesterol and LDL-cholesterol levels in normolipidemic men. Although a diet containing native shrimp has many benefits for healthy persons, but we do not recommend cultured shrimp in a healthy heart diet for persons with dyslipidemia or cardiovascular diseases.

  8. Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil

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    Lidiane Roberta Cruz da Silva

    2011-03-01

    Full Text Available Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the isolated species are known as potentially pathogenic to humans and other animals. Eighteen isolates of A. flavus and two of A. parasiticus were able to produce aflatoxin B and 33 out of the 46 species produced protease, indicating that these fungi may also become pathogenic to shrimp and their consumers.

  9. Membership, binarity, and rotation of F-G-K stars in the open cluster Blanco 1

    Science.gov (United States)

    Mermilliod, J.-C.; Platais, I.; James, D. J.; Grenon, M.; Cargile, P. A.

    2008-07-01

    Context: The nearby open cluster Blanco 1 is of considerable astrophysical interest for formation and evolution studies of open clusters because it is the third highest Galactic latitude cluster known. It has been observed often, but so far no definitive and comprehensive membership determination is readily available. Aims: An observing programme was carried out to study the stellar population of Blanco 1, and especially the membership and binary frequency of the F5-K0 dwarfs. Methods: We obtained radial-velocities with the CORAVEL spectrograph in the field of Blanco 1 for a sample of 148 F-G-K candidate stars in the magnitude range 10 rate reaches 40% (27/68) if one includes the photometric binaries. The cluster mean heliocentric radial velocity is +5.53 ± 0.11 km s-1 based on the most reliable 49 members. The V sin i distribution is similar to that of the Pleiades, confirming the age similarities between the two clusters. Conclusions: This study clearly demonstrates that, in spite of the cluster's high Galactic latitude, three membership criteria - radial velocity, proper motion, and photometry - are necessary for performing a reliable membership selection. Furthermore, even with accurate and extensive data, ambiguous cases still remain. Based on observations collected with the Danish 1.54-m and the Swiss telescopes at the European Southern Observatory, La Silla, Chile, and with the old YALO 1-m telescope at the Cerro Tololo InterAmerican Observatory, Chile. Table [see full textsee full textsee full textsee full textsee full textsee full text] is also available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/485/95

  10. May 2013 Groundwater and Surface Water Sampling at the Rio Blanco, Colorado, Site (Data Validation Package)

    Energy Technology Data Exchange (ETDEWEB)

    Hutton, Rick [S.M. Stoller Corporation, Broomfield, CO (United States)

    2013-10-01

    Annual sampling was conducted at the Rio Blanco, Colorado, site for the Long-Term Hydrologic Monitoring Program May 14-16, 2013, to monitor groundwater and surface water for potential radionuclide contamination. Sampling and analyses were conducted as specified in Sampling and Analysis Plan for the U.S. Department of Energy Office of Legacy Management Sites (LMS/PRO/S04351, continually updated). A duplicate sample was collected from location CER #1 Black Sulphur. Samples were analyzed for gamma-emitting radionuclides by high-resolution gamma spectrometry and for tritium using the conventional and enrichment methods.

  11. ANÁLISIS DE ACEPTACIÓN Y PREFERENCIA DEL MANJAR BLANCO DEL VALLE

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    JUAN SEBASTIÁN RAMÍREZ-NAVAS

    2014-06-01

    Full Text Available Se analizó sensorialmente muestras de Manjar Blanco, un tipo de dulce de leche colombiano, provistas por cuatro empresas tradicionales. Se realizaron pruebas de preferencia y aceptación con 76 consumidores, pertenecientes a la población estudiantil de la Universidad Santiago de Cali. Las edades de los participantes estuvieron entre los 15 y 70 años y el estratos socioeconómicos del 1 al 6. Se aplicó la prueba de selección y la prueba hedónica de 9 puntos en la que se calificó atributos como olor, color, sabor y textura. Los datos obtenidos se analizaron estadísticamente usando las pruebas de Friedman y Wilcoxon. Los resultados demuestran que la muestra D fue la preferida en un 35,53%, también esta muestra fue la que obtuvo las más altas calificaciones en olor (6,07±1,86, sabor (6,79±2,01 y textura (6,53±2,06, mientras que el color de la muestra C fue la de mayor agrado (6,72±1,96. Se concluye que para los consumidores el Manjar Blanco de la marca D fue el preferido sobre las otras marcas evaluadas sensorialmente.

  12. LAHAN BASAH BUATAN SEBAGAI MEDIA PENGOLAHAN AIR LIMBAH BUDIDAYA UDANG VANAME (Litopenaeus vannamaei BERSALINITAS RENDAH (Constructed Wetland for Remediation of Brackish Wastewater from White Shrimp (Litopenaeus vannamaei Cultivation

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    Syafrudin Raharjo

    2015-07-01

    Full Text Available ABSTRAK Air limbah budidaya udang berjumlah relatif banyak dan mengandung bahan pencemar yang berpotensi mencemari lingkungan. Di sisi lain, air limbah tersebut dapat diolah dan diresirkulasi dalam sistem budidaya udang. Tujuan penelitian ini adalah untuk menyelidiki kemampuan sistem lahan basah buatan-aliran air permukaan (LBB-AAP yang ditanami dengan rumput vetiver (Chrysopogon zizanioides, L dalam menghilangkan pencemar (NO2-, NO3-, NH3, NH4+ dan PO43- dari air limbah budidaya udang vaname (Litopenaeus vannamaei kondisi mesohaline dan mengevaluasi kinerja sistem tersebut. Hasil penelitian menunjukkan bahwa sistem LBB-AAP mampu mengeliminasi parameter NO2-, NO3-, NH3, NH4+ dan PO43- secara signifikan. Rumput vetiver mampu tumbuh pada kondisi mesohaline dan dapat melakukan remediasi air limbah tersebut. Serapan rumput vetiver dalam sistem LBB-AAP untuk NO3-, NH4+ dan PO43-adalah 28, 63 dan 83 %. Desain konstruksi LBB-AAP tipe Hidroponik menunjukkan kinerja terbaik dalam pengendalian air limbah budidaya udang vaname dibandingkan dengan tipe emergent, kombinasi hidroponik dan emergent. ABSTRACT The amount of wastewater shrimp cultivation is relatively/too much, contains a variety of pollutants and potentially pollute the environment. In other side, The wastewater can be treated and also recirculated in shrimp cultivation systems. The purpose of research is to investigate the ability of flow water surface-constructed wetland system (FWS-CWs that planted vetiver grass (Chrysopogon zizanioides, L that removes of pollutants (NO2-, NO3-, NH3, NH4+ and PO43- from wastewater vaname shrimp cultivation (Litopenaeus vannamaei on conditions mesohaline and with the aim of evaluating the performance of the system. The results of the research indicate that FWS-CWs able to eliminate the parameters significantly of NO2-, NO3-, NH3, NH4+ and PO43-. Vetiver grass could grow on mesohaline conditions and it can perform remediation of the wastewater. Uptake of

  13. Effects of methamidophos on acetylcholinesterase activity, behavior, and feeding rate of the white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    García-de la Parra, L M; Bautista-Covarrubias, J C; Rivera-de la Rosa, N; Betancourt-Lozano, M; Guilhermino, L

    2006-11-01

    The toxicity of methamidophos on the shrimp Litopenaeus vannamei was evaluated using acetylcholinesterase (AChE) activity, behavior, and feeding rate as effect criteria. The biochemical characterization of the soluble cholunesterase (ChE) present in both muscle and eye tissues of L. vannamei was performed in a first phase of the study. In both tissues, almost full inhibition of enzyme activity by eserine sulfate was found, indicating that the measured activity is mainly from ChE and not from other esterases. The highest rate of substrate hydrolysis was found when acetylthiocholine was used as substrate. To evaluate the effects of methamidophos on L. vannamei AChE, behavior, and feeding rate, shrimps were exposed for 24h to several sublethal concentrations of methamidophos. Significant effects of the pesticide on behavior and AChE were found, with behavior being a more sensitive endpoint than AChE inhibition. Feeding rate was not a sensible endpoint under conditions tested.

  14. Computational analysis and structure predictions of CHH-related peptides from Litopenaeus vannamei.

    Science.gov (United States)

    Nagaraju, G Purna Chandra; Kumari, N Siva; Prasad, G L V; Naik, B Reddya; Borst, D W

    2011-03-01

    The crustaceans produce several related peptides that belong to the crustacean hyperglycemic hormone (CHH) family. While these peptides have similar amino acid sequences, they have diverse biological functions that must arise, in part, from differences in the 3D shape of these peptides. However, it is generally accepted that peptides with a high degree of sequence similarity also have a similar 3-D structure. We used the solution structure of one peptide in the crustacean hyperglycemic hormone family, the molt-inhibiting hormone of the kuruma prawn (Marsupenaeus japonicus), to predict the shape of the five known peptides related to CHH in the Pacific white shrimp, Litopenaeus vannamei. The high similarity of the 3-D structures of these peptides suggests a common fold for the entire family. Nevertheless, minor differences in the shape of these peptides were observed, which may be the basis for their different biological properties.

  15. THE EFFECT OF FEEDING Lactobacillus ON GROWTH, SURVIVAL RATE AND PROTEASE ACTIVITY OF Litopenaeus vannamei

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    Nunak Nafiqoh

    2011-12-01

    Full Text Available This study examined the effect of two Lactobacillus bacteria on protease activity and growth rate of Litopenaeus vannamei. An experiment was conducted to examine protease activity and growth rate. The experiment consisted of two treatment tanks, the first tank was provided with artemia immersed in 2.6 x 1016 cfu/mL of bacteria solution, the second tank served as the control tank. After 20 days, the L. vannamei in the tank that received Lactobacillus have significantly different in growth, survival rate and protease activity (P<0.05 compared to the control, but no significant difference between Lactobacillus casei and Lactobacillus plantarum treatments. Within the digestive organ, protease activity of hepatopancreas and stomach demonstrated significant higher activity (P<0.05 compared to the intestine.

  16. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei)

    Science.gov (United States)

    Wilson-Sanchez, Griselda; Moreno-Félix, Carolina; Velazquez, Carlos; Plascencia-Jatomea, Maribel; Acosta, Anita; Machi-Lara, Lorena; Aldana-Madrid, María-Lourdes; Ezquerra-Brauer, Josafat-Marina; Robles-Zepeda, Ramón; Burgos-Hernandez, Armando

    2010-01-01

    An organic extract from fresh shrimp (Litopenaeus vannamei) was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line (B-cell lymphoma), respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC) and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line. PMID:21139845

  17. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei

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    Carolina Moreno-Félix

    2010-11-01

    Full Text Available An organic extract from fresh shrimp (Litopenaeus vannamei was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9 and a cancer cell line (B-cell lymphoma, respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line.

  18. Hemocyanin-derived phenoloxidase activity is dependent on dodecameric structure in shrimp Litopenaeus vannamei

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    Wang Ke-Zhou

    2015-01-01

    Full Text Available Hemocyanin (Hc is a multifunctional protein in both mollusks and arthropods. Phenoloxidase (PO activities are the most important physiological functions for Hcs after conversion. In shrimp, Hc occurs as two oligomer forms, dodecamers and hexamers. Differences in the transport oxygen capacity and agglutination activity between the two oligomers of shrimp Hc have been found. In the present study, we investigated the differences in the Hc-derived PO activity between the dodecameric and hexameric Hc forms of the shrimp Litopenaeus vannamei. The two oligomers were separated by non-denaturing polyacrylamide gel electrophoresis, converted by trypsin cleavage and their PO activities were determined by oxidation of L-DOPA. The dodecamers exhibited PO activity after enzymatic conversion while the hexamers did not exhibit PO activity. This result provides new insight into the structural/functional relationships of Hcs.

  19. Symmetric frenzy and catastrophic change: a consideration of primitive mental states in the wake of Bion and Matte Blanco.

    Science.gov (United States)

    Lombardi, Riccardo

    2009-06-01

    The author explores the connections between Matte Blanco's notion of symmetric frenzy, i.e. the turbulence characteristic of the deepest levels of mental functioning, and Bion's concept of catastrophic change. For Bion, mental links are retrieved from the formless darkness of infinity. With catastrophic change, emotional violence and the confining nature of representation come into conflict, leaving the subject prey to an explosiveness that paralyses mental resources. Matte Blanco identifies indivisibility as the abyss in which all differentiation ceases; he bases his model on the conflict between symmetry and asymmetry. Infinity, he maintains, is where the first forms of mentalization develop. Both Bion and Matte Blanco emphasize the contrast between the immensity of mental space and the spatio-temporal order introduced by the activation of thinking functions. The author presents clinical material from the analysis of a psychotic patient, stressing the need to encourage both working through the defect of thinking (Bion) and 'unfolding' manifestations of symmetry (Matte Blanco) so as to foster the activation of the resources of thought, meanwhile postponing transference interpretation. He concludes with two later sessions, in which recognition of the analyst in the transference allows the analysand to develop his capacity for containment and asymmetric differentiation.

  20. IMPACT OF PARASITISM BY GREGARINES (Nematopsis sp IN FARMING SHRIMP Litopenaeus vannamei

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    Francisco M. Guzmán-Sáenz

    2014-07-01

    Full Text Available Infestation of gregarines from genus Nematopsis in Pacific white shrimp Litopenaeus vannamei is commonly associated with a decrease in production and low weight, as well as a possible predisposition to viral infections. The goal of our past research was to evaluate the effect of the Nematopsis sp. gregarine parasitosis on the growth of farm-grown Litopenaeus vannamei shrimp. Four cages were built with PVC pipes and plastic screens with a 6mm light diameter, 1,7m in height, and 1,5m in width and length (2,23 m3, and were installed in a farm-growing area in La Pesca, Tamaulipas. Twenty-nine previously weighed shrimp, L. vannamei, were placed in each cage. These had a “2” (6 to 10 parasites per shrimp degree of infestation severity by Nematopsis sp. The shrimp in 2 cages were fed for 5 days with the farm feed and 6g/kg of sodium monensin. The shrimp in the remaining cages were fed with commercial feed. After 5 days, the shrimp in the 4 cages were fed with a balanced feed for 38 days. After this, the biomass of each cage was recorded. The shrimp in the control cages weighed an average of 7,12g at the beginning of research and 9,27g at the end, and the treated shrimp weighed 7,09g and 9,88g, respectively. The non-treated shrimp gained 2,5g and the treated shrimp gained 2,79g. This present research shows that the negative effect on the shrimp growth caused by Nematopsis sp. infestation can be reversed if the infected shrimp are treated against infestation with sodium monensin, which possesses a therapeutic effect that eliminated the infestation without affecting the shrimp growth.

  1. Osmoregulatory capacity of the shrimp Litopenaeus vannamei at different temperatures and salinities, and optimal culture environment

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    L. Fernando Bückle

    2006-09-01

    Full Text Available Osmoregulation in Litopenaeus vannamei was studied in a factorial experiment at four temperatures (20, 24, 28 and 32 °C and six salinities (10, 16, 22, 28, 34 and 40 ‰. The isosmotic related points for 20, 24, 28, and 32 °C were 754, 711, 822, and 763 mmol/kg, respectively. This species hyperregulates between at salinities of 10 and 20 ‰ and hyporegulates between 20 and 40 ‰. The isosmotic point in L. vannamei exposed to constant salinities changed in relation to temperature from 717 to 823 mmol/kg. For these experimental conditions, the T-S combination of 32 °C and 28 ‰ produced the best growth. Rev. Biol. Trop. 54 (3: 745-753. Epub 2006 Sept. 29.La respuesta osmorreguladora de Litopenaeus vannamei se estudió en un experimento factorial con cuatro temperaturas (20, 24, 28 y 32 ºC y seis salinidades (10, 16, 22, 28, 34 y 40 ‰. Los puntos isosmóticos relacionados para 20, 24, 28, y 32 ºC fueron 754, 711, 822, y 763 mmol/kg, respectivamente. Esta especie hiperregula dentro del intervalo de 10 y 20 ‰ e hiporegula entre 20 y 40 ‰. El punto isosmótico de L. vannamei expuesto a salinidades constantes cambia en relación a la temperatura desde 717 a 823 mmol/kg. Para estas condiciones experimentales, la combinación T-S de 32 ºC y 28 ‰ produjo el mejor crecimiento.

  2. Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei

    International Nuclear Information System (INIS)

    Peng, Ting; Wang, Wei-Na; Gu, Mei-Mei; Xie, Chen-Ying; Xiao, Yu-Chao; Liu, Yuan; Wang, Lei

    2015-01-01

    Highlights: • Cd 2+ induces Cdc42 and MAPKs pathway related gene of Litopenaeus vannamei up-regulation. • Reduction of THC, increase of ROS production and apoptotic cell rate were observed when the shrimps exposure to Cd 2+ . • DsRNA-suppression of LvCdc42 and MAPKs during Cd 2+ stress reduces the ROS production and apoptosis. • We conclude that LvCdc42 and MAPKs play key roles in Cd 2+ stress responses of shrimps. - Abstract: Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals’ stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps’ responses to Cd 2+ . They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses

  3. Enhancement of non-specific immune response, resistance and growth of (Litopenaeus vannamei by oral administration of nucleotide

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    Henky Manoppo

    2011-01-01

    Full Text Available This research evaluated the nonspecific immune responsse, resistance, and growth of Litopenaeus vannamei fed nucleotide diet. Shrimp juveniles (mean weight 5.39±0.56 g were reared in two groups of glass aquaria, each with three replications. Shrimps in group one and group two were fed nucleotide diet and basal diet each for four weeks. Total haemocyte count (THC and PO activity were evaluated at the end of feeding while growth was measured at two weeks interval. At the end of feeding trial, the shrimps were intramuscularly injected with Vibrio harveyi 0.1x106 cfu.shrimp-1. THC of shrimp fed nucleotide diet significantly increased (P-1 diet showed positive effect on the enhancement of nonspecific immune responsse, resistance, and growth of L. vannamei.  Key words: Litopenaeus vannamei, nucleotide, THC, PO activity, resistance   ABSTRAK Penelitian bertujuan untuk mengevaluasi respons imun non-spesifik dan resistensi udang vaname (Litopenaeus vannamei yang diberi pakan nukleotida.  Juvenil (5,39±0,56 g dipelihara dalam dua kelompok akuarium kaca masing-masing dengan 3 ulangan.  Udang dalam dalam kelompok pertama diberi pakan nukleotida sedangkan udang dalam kelompok kedua diberi pakan standar selama 4 minggu. Total haemocyte count (THC dan aktivitas phenoloxidase (PO diukur pada akhir pemberian pakan sedangkan pertumbuhan udang diukur setiap dua minggu. Pada akhir periode pemberian pakan perlakuan, udang diuji tantang secara injeksi intramuskular dengan bakteri Vibrio harveyi 0,1x106 cfu.udang-1. THC udang yang diberi pakan nukleotida meningkat secara signifikan (P-1 pakan selama 4 minggu memberi pengaruh positif terhadap peningkatan respons imun non-spesifik, resistensi dan pertumbuhan udang vaname. Kata kunci: Litopenaeus vannamei, nukleotida, THC, aktivitas PO, resistensi

  4. Growth and immune response of Litopenaeus vannamei fed on β-(1, 3 glucan and poly-β hydroxybutyrate

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    , Sarmin

    2015-05-01

    Full Text Available ABSTRACT This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei fed on the diet supplemented with β-(1,3 glucan (BG and poly-β-hydroxybutyrate (PHB as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives, 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response. Keywords: growth, shrimp, non-specific immune response, Litopenaeus vannamei  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja pertumbuhan dan respons imun nonspesifik udang vaname Litopenaeus vannamei yang diberi pakan dengan penambahan feed additive berupa β-(1,3 glukan (BG dan poli-β-hidroksibutirat (PHB. Juvenil udang 2,06±0,03 g dipelihara pada 12 unit akuarium dengan empat perlakuan dan tiga ulangan, serta padat tebar 20 ekor/tank selama 42 hari pemeliharaan. Perlakuan yang diberikan dalam penelitian ini yaitu penambahan BG (1,5 g/kg, PHB (10 g/kg, dan BG (1,5 g/kg+PHB (10 g/kg, serta kontrol (tanpa penambahan feed additive. Hasil penelitian menunjukkan bahwa udang yang diberi 1,5 g/kg BG memiliki kinerja pertumbuhan dan respons imun nonspesifik yang terbaik. Kata kunci: pertumbuhan, udang, respons imun nonspesifik, Litopenaeus vannamei

  5. Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei

    OpenAIRE

    Yi Gao; Jiankai Wei; Jianbo Yuan; Xiaojun Zhang; Fuhua Li; Jianhai Xiang

    2017-01-01

    Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology...

  6. Amphibians and Reptiles from Reserva Natural Absoluta Cabo Blanco, province of Puntarenas, Costa Rica

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    Laurencio, D.

    2009-01-01

    Full Text Available A survey of the amphibians and reptiles of Reserva Natural Absoluta Cabo Blanco (RNACB, Puntarenas, Costa Ricawas conducted from May to August 2003. Thirteen amphibian and 19 reptile species were found within the RNACBboundaries. Twenty-two of these species were not previously recorded from the lower Nicoya Peninsula and for seven,this locality represents the southernmost extension of their range. One additional amphibian and three reptile species areknown from the area based on literature review and examination of museum collections. However, interviews withlocals indicate that up to nine other reptile species could be present in the reserve. I observed all but one amphibianspecies at Laguna Balsitas and at least eight species utilize the lagoon for reproduction. The lagoon is also notable forits population of Kinosternon scorpioides. This study will provide a baseline for further research in the reserve.

  7. The indistrialization of Salins Peñon Blanco case lagoon Santa Maria

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    Xochitl Minerva Guevara Correa

    2015-01-01

    Full Text Available The Lagoon de Santa Maria is one of the twelve lagoons or salt deposits that formed part from the colonial, of a production unit known as Salinas del Peñón Blanco, the name given by its proximity to a hill, which is white in appearance; its production of salt was by solar evaporation system, brought from Europe, which was used in lakes and sea path Mexico to inland lakes in 1845 is introduced by José María Errazu this new technology understood as the systematic knowledge and components such as materials, processes, labor, the productive end, where sometimes the technology is embodied in this example as architecture. 

  8. Efecto de tres tipos de presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus

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    Martha Prieto-Guevara

    2013-11-01

    Full Text Available Objetivo. Evaluar el efecto de diferentes presas vivas en la larvicultura de bagre blanco (Sorubim cuspicaudus. Materiales y métodos. Al inicio de la alimentación exógena de Sorubim cuspicaudus, se ofreció zooplancton producido en mesocosmos (T1, zooplancton silvestre (T2 y nauplios de Artemia (T3, en concentración de 10.000 zoop/L, dos veces al día, durante seis días. Se utilizaron 18 acuarios de cinco litros de volumen útil, con densidad de 25 Larvas/L, seis réplicas por tratamiento en un diseño al azar. Se estimaron la ganancia en peso (Gp y longitud (Gl, tasa de crecimiento específico (G, sobrevivencia (S, resistencia al estrés (Re, mortalidad acumulada (Ma y mortalidad por canibalismo (Mc. Resultados. Las larvas alimentadas con mesocosmos presentaron la mayor sobrevivencia (81.3±15.9%; aunque el mejor crecimiento lo presentaron las larvas alimentadas con zooplancton silvestre (T2 las cuales presentaron la mayor mortalidad (42.0±10.7% y la menor resistencia al estrés (30.0±33.0%. El canibalismo se observó en todos los tratamientos, oscilando entre 4.0 (T2 y 14.3% (T1 sin diferencias significativas entre estos valores (p>0.05. Conclusiones. El uso de zooplancton producido bajo condiciones controladas permitió una alta sobrevivencia, adecuado desempeño y resistencia de las larvas, perfilándose como alternativa viable en la primera alimentación de bagre blanco.

  9. The involvement of bacterial quorum sensing in the spoilage of refrigerated Litopenaeus vannamei.

    Science.gov (United States)

    Zhu, Suqin; Wu, Haohao; Zeng, Mingyong; Liu, Zunying; Wang, Ying

    2015-01-02

    Quorum-sensing signals in refrigerated shrimp (Litopenaeus vannamei) undergoing spoilage were examined using bioreporter assays, thin-layer chromatography and gas chromatography-mass spectrometry, and the results revealed the presence of three types of autoinducers including acetylated homoserine lactones (AHLs) (i.e., N-hexanoyl-homoserine lactone, N-oxohexanoyl-homoserine lactone and N-octanoyl-homoserine lactone), autoinducer-2, and cyclic dipeptides (i.e., cyclo-(L-Pro-L-Leu), cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe)). Autoinducer-2, rather than any AHL, was detected in extracts from pure cultures of the specific spoilage organisms (SSO), i.e., Shewanella putrefaciens (SS01) and Shewanella baltica (SA02). As for the cyclic peptides, only SA02 was determined to produce cyclo-(L-Pro-L-Leu). According to the transcription levels of LuxR (the master quorum-sensing regulator) in the SSO in response to exogenous autoinducers, the SSO could sense AHLs and cyclo-(L-Leu-L-Leu), rather than autoinducer-2, cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe). In accordance with the results of LuxR expression, the production of biofilm matrixes and extracellular proteases in the SSO was regulated by exogenous AHLs and cyclo-(L-Pro-L-Leu), rather than 4,5-dihydroxy-2,3-pentanedione (the autoinducer-2 precursor), cyclo-(L-Leu-L-Leu) and cyclo-(L-Pro-L-Phe). Exogenous N-hexanoyl-homoserine lactone and cyclo-(L-Pro-L-Leu) increased the growth rates and population percentages of the SSO in shrimp samples under refrigerated storage, and interestingly, exogenous 4,5-dihydroxy-2,3-pentanedione also increased the population percentages of the SSO in vivo by inhibiting the growth of the competing bacteria. However, according to the levels of TVB-N and the volatile organic components in the shrimp samples, exogenous 4,5-dihydroxy-2,3-pentanedione did not accelerate the shrimp spoilage process as N-hexanoyl-homoserine lactone and cyclo-(L-Pro-L-Leu) did. In summary, our results suggest that

  10. Aquacultural importance of the integumental pore pattern in postlarval whiteleg shrimp Litopenaeus vannamei (Decapoda: Penaeidae

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Cabrera-Jiménez

    2006-09-01

    Full Text Available The analysis of integumental pore pattern has identified two intraspecific groups of postlarval shrimp Litopenaeus vannamei (Boone, 1931, a species of which three to five natural populations or groups are expected to be found from Mexico to Peru. Natural distribution and man-made redistributions have not been typified on a population basis in any penaeid, but it is important to do so in this commercially significant group. Important factors to be defined on a geographic population or group basis include differences in domesticity (eg resistance to pathological agents; food conversion indexes and food preferences; growth indices; tolerance to stress of various origins and other attributes useful in aquaculture. This consideration is valid not only for L. vannamei but also for any other shrimp species that has been the object of artificial redistribution. Rev. Biol. Trop. 54 (3: 707-709. Epub 2006 Sept. 29.Mediante el análisis del patrón de poros tegumentarios ha sido posible caracterizar a dos grupos intraespecíficos en dos estadios postlarvales del camarón Litopenaeus vannamei. Se estima que se podrán encontrar entre tres y cinco grupos o poblaciones naturales en el área de distribución natural desde México hasta Perú. En ningún peneido se ha tipificado a los grupos o poblaciones naturales, lo cual es muy importante no solamente en relación con las poblaciones en su medio natural, sino también en relación a las redistribuciones hechas por el ser humano. Esto se debe a las implicaciones acuiculturales y a las diferencias en domesticidad, como la resistencia o sensibilidad a enfermedades virales y a otros agentes patógenos, el factor de conversión y las preferencias alimenticias, el índice de crecimiento, la tolerancia al estrés de diversos orígenes y otros atributos útiles en la acuicultura, todos los cuales no están definidos aún sobre la base de grupos o poblaciones geográficas. Esta consideración es válida no solamente

  11. GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves

    OpenAIRE

    Franelyne Pataueg Casuga; Agnes Llamasares Castillo; Mary Jho-Anne Tolentino Corpuz

    2016-01-01

    Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica (Blanco) (Moraceae) (B. luzonica), an endemic plant in the Philippines. Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane, ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected...

  12. Citrus Essential Oil of Nigeria Part IV: Volatile Constituents of Leaf Oils of Mandarins (Citrus Reticulata Blanco) From Nigeria

    OpenAIRE

    Adeleke A. Kasali; Oladipupo A. Lawal; Olatunji T. F. Abanikannda; Abayomi A. Olaniyan; William N. Setzer

    2010-01-01

    The chemical composition of hydrodistilled oils obtained from the leaves of six Citrus reticulata Blanco (mandarin) cultivars grown in Nigeria were examined by GC and GC/MS, the result of their chemical composition were further submitted to cluster analysis. Fifty seven constituents were characterized accounting for 88.2 - 96.7% of the total oils. Sabinene, g -terpinene, P-cymene, d -3-carene and (E)- b -ocimene were observed in great variability in all the oils. Other constituents include li...

  13. Irradiation effect on α- and β-caseins of milk and Queso Blanco cheese determined by capillary electrophoresis

    International Nuclear Information System (INIS)

    Ham, J.S.; Jeong, S.G.; Lee, S.G.; Han, G.S.; Chae, H.S.; Yoo, Y.M.; Kim, D.H.; Lee, W.K.; Jo, C.

    2009-01-01

    Milk and Queso Blanco cheese were exposed to irradiation with doses of 1, 2, 3, 5, and 10 kGy to investigate the irradiation effect on α- and β-casein using a capillary electrophoresis. α S1 -Casein to total protein ratio in raw milk was decreased from 19.63% to 8.64% by 10 kGy of gamma irradiation. The ratio of α S1 - to α S0 -casein was also decreased from 1.38 to 0.53, which showed α S1 -casein is more susceptible to gamma irradiation than α S0 -casein. Similarly, α S1 -casein to total protein ratio in Queso Blanco cheese was decreased from 17.48% to 7.82% and the ratio of α S1 - to α S0 -casein was decreased from 1.16 to 0.43 by 10 kGy of gamma irradiation. Dose-dependent reduction of β A1 -casein was also found. β A1 -Casein to total protein ratios in raw milk and Queso Blanco cheese were decreased from 22.00% to 14.16% and from 21.96% to 13.89% after 10 kGy, respectively. The ratios of β A1 - to β A2 -casein were from 1.10 to 0.64 and 0.93 to 0.57 in milk and Queso Blanco cheese, respectively. However, α S0 -, β B -, and β A3 -casein increased by irradiation at 10 kGy. The results suggest that α S1 -casein and β A1 -casein were more susceptible to gamma irradiation, and may be related to the reduction of milk allergenicity caused by gamma irradiation

  14. Characterization of hydrology and water quality of Piceance Creek in the Alkali Flat area, Rio Blanco County, Colorado, March 2012

    Science.gov (United States)

    Thomas, Judith C.

    2015-12-07

    Previous studies by the U.S. Geological Survey identified Alkali Flat as an area of groundwater upwelling, with increases in concentrations of total dissolved solids, and streamflow loss, but additional study was needed to better characterize these observations. The U.S. Geological Survey, in cooperation with the Bureau of Land Management, White River Field Office, conducted a study to characterize the hydrology and water quality of Piceance Creek in the Alkali Flat area of Rio Blanco County, Colorado.

  15. Litopenaeus vannamei

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-20

    Jul 20, 2009 ... Control groups were consisted of i) shrimp fed diet with commercial probiotic as positive control, and ii) shrimp fed ... quality, survival rate and digestive enzyme activities was investigated at .... Effect of basal diet supplemented with B. subtilis and commercial probiotics on growth performance of. L. vannamei ...

  16. Comparative transcriptional survey between self-incompatibility and self-compatibility in Citrus reticulata Blanco.

    Science.gov (United States)

    Ma, Yuewen; Li, Qiulei; Hu, Guibing; Qin, Yonghua

    2017-04-20

    Seedlessness is an excellent economical trait, and self-incompatibility (SI) is one of important factors resulting in seedless fruit in Citrus. However, SI molecular mechanism in Citrus is still unclear. In this study, RNA-Seq technology was used to identify differentially expressed genes related to SI reaction of 'Wuzishatangju' (Citrus reticulata Blanco). A total of 35.67GB raw RNA-Seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and N50 value of 1549. Twenty-three candidate unigenes related to SI were analyzed using qPCR at different tissues and stages after self- and cross-pollination. Seven pollen S genes (Unigene0050323, Unigene0001060, Unigene0004230, Unigene0004222, Unigene0012037, Unigene0048889 and Unigene0004272), three pistil S genes (Unigene0019191, Unigene0040115, Unigene0036542) and three genes (Unigene0038751, Unigene0031435 and Unigene0029897) associated with the pathway of ubiquitin-mediated proteolysis were identified. Unigene0031435, Unigene0038751 and Unigene0029897 are probably involved in SI reaction of 'Wuzishatangju' based on expression analyses. The present study provides a new insight into the molecular mechanism of SI in Citrus at the transcriptional level. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Slippery surfaces of carnivorous plants: composition of epicuticular wax crystals in Nepenthes alata Blanco pitchers.

    Science.gov (United States)

    Riedel, Michael; Eichner, Anna; Jetter, Reinhard

    2003-11-01

    Plants in the genus Nepenthes obtain a substantial nutrient supply by trapping insects in highly modified leaves. A broad zone of the inner surface of these pitchers is densely covered with wax crystals on which most insects lose their footing. This slippery wax surface, capturing prey and preventing its escape from the trap, plays a pivotal role in the carnivorous syndrome. To understand the mechanism of slipperiness, the present investigation aimed at an ultrastructural and physico-chemical characterization of the wax crystals in pitchers of N. alata Blanco. Scanning electron microscopy revealed that entire platelets protruded perpendicularly from the surface. Methods were developed that allowed the mechanical removal of wax crystals from the pitcher surface. It could be shown that the sampling was selective for the epicuticular wax, relevant for plant-insect interactions. The crystals consisted of a mixture of aliphatic compounds dominated by very-long-chain aldehydes. Triacontanal, at 43% the most abundant constituent, was largely responsible for crystal formation. Solubility data indicate that the Nepenthes crystals contained polymeric forms of this aldehyde. The resulting mechanical properties of the polymer crystals and the mechanism of slipperiness are discussed.

  18. Forensic Hydrological Investigation of the Blanco River Flood May 2015, Wimberley, TX

    Science.gov (United States)

    Furl, C.

    2015-12-01

    A forensic hydrological investigation of a major flash flood was conducted for the Blanco River in south-central Texas. The unprecedented flood occurred during the early morning hours of May 24th leaving 12 dead in the towns of Wimberley and San Marcos. Hundreds of homes were damaged or destroyed, two reinforced concrete bridges were washed off their piers, and nearly 100 high water rescues were made the following day. The present work characterizes the meteorological setup leading to the event, describes the flood hydrology using the Gridded Surface Subsurface Hydrologic Analysis (GSSHA) model, and reports on an extensive field campaign seeking to document high water marks throughout the 1200 km2 basin. Results indicate high precipitable water values, large CAPE, and strong mid and upper level winds aided in impressive divergence over the region. This allowed for storms to continually produce heavy rainfall over the same areas. Large regions of the catchment received greater than 200 mm across the upper portion of the basin with 24 hr maximums around 330 mm. GSSHA simulations indicate good performance when compared to a stage hydrograph recorded mid-catchment. The remaining USGS gauges failed early on during the rising limb of the hydrograph. Model estimates indicate peak streamflow was approximately 5500 cms with stage values nearing 13 m as the flood wave moved through the town of Wimberley. Approximately 125 locations were examined for high water marks along the mainstem of the river using RTK GPS. Stage values ranged from 12 - 18 m.

  19. Comparison of predicted and observed pore pressure increases on Rio Blanco

    International Nuclear Information System (INIS)

    Banister, J.R.; Ellett, D.M.; Pyke, R.; Winters, L.

    1976-01-01

    The RIO BLANCO event presented the opportunity to monitor, under controlled conditions in the field, the increase in pore pressures resulting from ground motion similar to an earthquake. In situ measurements of pore pressure changes were made by Sandia Laboratories and Dames and Moore. This report contains the results of laboratory tests believed to be indicative in assessing the magnitude of pore pressure increases and probability of soil liquefaction. These include triaxial load tests, gradation of grain size, and relative density. No liquefaction was observed in the field, and the increase of in situ pore pressures were much less than expected from laboratory measurements. Allied subjects presented in this report are pore pressure propagation and dissipation profiles, the previously unpublished pore pressure measurements made by Dames and Moore, and the boring logs for the various sites where measurements were taken. It is concluded that methods used to predict pore pressure increases and liquefaction potential are overly conservative, at least for these alluvial and colluvial soils found in Colorado

  20. Effect of Citrus reticulata Blanco Essential Oil on Cryptolestes ferrugineus (Stephens) Adults.

    Science.gov (United States)

    Lü, Jianhua

    2017-12-01

    The rusty grain beetle, Cryptolestes ferrugineus (Stephens), is a major insect pest of stored products worldwide. In an effort to manage populations of C. ferrugineus in stored grains, the essential oil from Citrus reticulata Blanco peel was prepared by the Soxhlet method with anhydrous diethyl ether, and its contact activity, repellent activity, and fumigant activity were investigated against C. ferrugineus adults. C. reticulata peel essential oil had potent contact, repellent, and fumigant activity against C. ferrugineus adults, with activity significantly increasing with increasing exposure dosage during the same exposure time. Percent repellency values achieved >80.0% after 60 h of exposure. For contact toxicity, the corrected mortality of C. ferrugineus adults reached >99.0% after 48 h of exposure at the dosage of 2.0 μL/cm 2 . For fumigant toxicity, the corrected mortality of C. ferrugineus adults reached 89.0 and 100.0% at the dosages of 40 and 80 μL/L of air, respectively. These results suggest that the C. reticulata peel essential oil has strong potential for managing populations of C. ferrugineus in practice.

  1. Correlaciones entre Pesos Vivos y Medidas Corporales del Ganado Blanco-Orejinegro a Diferentes Edades

    Directory of Open Access Journals (Sweden)

    Arboleda A. Oscar

    1979-12-01

    Full Text Available El ganado Blanco Orejinegro ofrece condiciones fisiológicas admirables para adaptarse a terrenos pobres y quebrados de nuestro medio, sobrevivir y reproducirse hasta una edad avanzada. Es por ello que presenta considerable ventaja para cruzamientos por cuanto posee cualidades de baja heredabilidad, que responden en forma óptima a heterosis; las cuales combinadas con otras características económicas deseables, aportadas por la otra raza constituyente del cruce, dan un híbrido de muchas ventajas para el productor comercial. El ganado BON ha sido manejado durante muchos años solo con fines de conservación y multiplicación, sin parámetros definidos de selección. Por lo tanto, el presente trabajo ha pretendido estudiar ciertas medidas corporales y su relación con pesos a diferentes edades, para así establecer constantes para la raza y curvas de crecimiento que sirvan de modelo y base de comparación con otras razas criollas colombianas.

  2. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Yu, Yang; Zhang, Xiaojun; Liu, Jingwen; Li, Fuhua; Huang, Hao; Li, Yijun; Liu, Xiaolin; Xiang, Jianhai

    2015-01-01

    Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.

  3. Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers

    Science.gov (United States)

    Zhang, Kai; Wang, Weiji; Li, Weiya; Zhang, Quanqi; Kong, Jie

    2014-08-01

    Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content ( PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles ( Na) and effective alleles ( Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity ( H o) values were lower than the expected heterozygosity ( H e) values (0.526-0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. F is values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise F st values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks (11.3%) was much lower than that within stocks (88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

  4. Nutritional value of various ray fish liver oils to the pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Perez-Velazquez, Martin; González-Félix, Mayra L; Navarro-García, Gerardo; Valenzuela-Escalante, Erasmo

    2008-11-01

    A 32-day comparative feeding trial was performed to evaluate the nutritional value of four different ray fish liver oils to the Pacific white shrimp Litopenaeus vannamei. Four feeds were prepared with liver oil extracted from Dasyatis brevis, Rhinoptera steindachneri, Aetobatus narinari, and R. bonasus. A control feed was prepared with Menhaden fish oil. Ray fish liver oils were mainly composed of poly- and highly unsaturated fatty acids and contained levels of 20:5n-3 and 22:6n-3 comparable in magnitude to those of Menhaden fish oil, except for A. narinari liver oil, which had moderately low concentrations of 20:5n-3 and 22:6n-3 but showed a particularly high level of 20:4n-6, more than six times greater than that of Menhaden fish oil. Dietary fatty acids significantly influenced the fatty acid composition of shrimp muscle tissue, e.g., the diet with Menhaden fish oil elicited significantly higher shrimp muscle DHA level than diets with oil from D. brevis, and A. narinari, but not than diets with oil from R. steindachneri and R. bonasus. In spite of these differences, all four ray fish liver oils evaluated were as efficient in promoting growth and survival of L. vannamei as Menhaden fish oil, an ingredient known for its adequate nutritional quality to shrimp and fish. This study demonstrated one of the many possible applications of a locally-available resource that is currently being wasted.

  5. Patógenos introducidos al Perú en post larvas de Litopenaeus vannamei importadas

    Directory of Open Access Journals (Sweden)

    Mervin Guevara

    2012-11-01

    Full Text Available El comercio internacional de animales acuáticos es uno de los factores más importantes en la introducción involuntaria de patógenos a una región libre de estos. Los patógenos introducidos han sido causa de enormes pérdidas económicas en organismos bajo cultivo y daños ecológicos en poblaciones silvestres. En este trabajo, utilizando la técnica de la PCR se analizaron postlarvas de Litopenaeus vannamei importadas desde Ecuador para ser utilizada en los cultivos de langostinos en Tumbes. La finalidad fue detectar patógenos como el virus de la mancha blanca (WSV, virus de la cabeza amarilla (YHV, virus de la necrosis hipodérmica y hematopoyética infecciosa (IHHNV, Baculovirus penaei (BP, virus del síndrome de Taura (TSV y la bacteria de la hepatopancreatitis necrotizante (NHPB. Se analizaron 177 muestras de postlarvas en el año 2009 y 274 en el 2010. En el año 2009 se encontraron cuatro patógenos infecciosos en las postlarvas analizadas, siendo más frecuente el IHHNV (9,60%. En el año 2010 la frecuencia de aparición de postlarvas infectadas con IHHNV fue mayor (16,79%. Tanto en el 2009 como en el 2010 se encontraron postlarvas positivas a NHPB, BP y TSV

  6. A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

    Directory of Open Access Journals (Sweden)

    Hao Wu

    2017-05-01

    Full Text Available Abstract White spot syndrome virus (WSSV cause great harm in shrimp aquaculture. To understand the impact of viral infection on the shrimp metabolism, we monitored the culture farms of Litopenaeus vannamei and collected the samples on different stages of WSSV infection. The hepatopancreas of shrimp were separated, and then used gas chromatography mass spectrometry to detect the metabolites. Through the mass spectrometric analysis combined with multivariate data analysis, including PCA and OPLS models, metabolism of the shrimp was significantly changed by WSSV infection. The data indicated that in the early stage of WSSV infection, the glycolysis changed significantly, the contents of glucose and lactate increased distinctly. The metabolites of TCA cycle did not show up obviously regularity. The organism of fatty acids showed the same situation with glycolysis. At the early stage of infection, 14 amino acids metabolism were up-regulated, and glycine still increased at later stage of infection and the concentration was increased twice. The data of this study may provide some information to further research of viral disease mechanism.

  7. Carotenoides totais em resíduos do camarão Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Larissa Mont'Alverne Jucá Seabra

    2014-02-01

    Full Text Available Considerando-se o possível aproveitamento dos resíduos, provenientes do beneficiamento do camarão, realizouse, neste trabalho, a determinação da concentração de carotenoides totais dos resíduos do camarão Litopenaeus vannamei e da farinha obtida após secagem. As análises foram realizadas nos resíduos in natura e na farinha recémprocessada (dia 0 e aos 60, 120 e 180 dias de armazenamento sob congelamento. Os resíduos frescos apresentaram, no dia 0, teores de 42,74 µg/g de carotenoides totais e, a farinha recém-processada, de 98,51 µg/g. Após 180 dias de armazenamento, sob congelamento, os teores de carotenoides totais diminuíram significativamente, quando comparados com os do dia 0 (p < 0,05.

  8. Transcriptome analysis of Litopenaeus vannamei in response to white spot syndrome virus infection.

    Directory of Open Access Journals (Sweden)

    Xiaohan Chen

    Full Text Available Pacific white shrimp (Litopenaeus vannamei is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown. In this study, the impact of WSSV infection on host gene expression in the hepatopancreas of L. vannamei was investigated through the use of 454 pyrosequencing-based RNA-Seq of cDNA libraries developed from WSSV-challenged shrimp or normal controls. By comparing the two cDNA libraries, we show that 767 host genes are significantly up-regulated and 729 genes are significantly down-regulated by WSSV infection. KEGG analysis of the differentially expressed genes indicated that the distribution of gene pathways between the up- and down-regulated genes is quite different. Among the differentially expressed genes, several are found to be involved in various processes of animal defense against pathogens such as apoptosis, mitogen-activated protein kinase (MAPK signaling, toll-like receptor (TLR signaling, Wnt signaling and antigen processing and presentation pathways. The present study provides valuable information on differential expression of L. vannamei genes following WSSV infection and improves our current understanding of this host-virus interaction. In addition, the large number of transcripts obtained in this study provides a strong basis for future genomic research on shrimp.

  9. Autolysis of Pacific white shrimp (Litopenaeus vannamei) meat: characterization and the effects of protein additives.

    Science.gov (United States)

    Eakpetch, P; Benjakul, S; Visessanguan, W; Kijroongrojana, K

    2008-03-01

    Autolytic activity of Pacific white shrimp (Litopenaeus vannamei) mince in the absence and in the presence of 2.5%NaCl was investigated. Pacific white shrimp mince exhibited the maximum autolytic activity at 35 and 40 degrees C in the absence and in the presence of 2.5%NaCl, respectively, as evidenced by the highest TCA-soluble peptide content and the greatest disappearance of myosin heavy chain (MHC). The autolysis was more pronounced in the acidic pH values, followed by alkaline pH ranges. Pepstatin A showed the highest inhibition toward autolysis in the acidic condition, revealing that aspartic proteinase was dominant in shrimp muscle. Nevertheless, soybean trypsin inhibitor effectively inhibited the autolysis at neutral and alkaline pH values, suggesting that serine proteinase was present in shrimp mince but contributed to autolysis at a lower extent in shrimp meat. Autolysis in shrimp meat could be inhibited partially by all protein additives, including bovine plasma protein (BPP), egg white (EW), and whey protein concentrate (WPC). The inhibition of autolysis increased when the level of protein additives increased with the concomitant increase in band intensity of MHC retained. WPC and BPP in the range of 2% to 3% exhibited the highest inhibition toward autolysis of shrimp mince.

  10. Glycosylation of hemocyanin in Litopenaeus vannamei is an antibacterial response feature.

    Science.gov (United States)

    Zhang, Zehui; Wang, Fan; Chen, Chuandao; Zheng, Zhou; Aweya, Jude Juventus; Zhang, Yueling

    2017-12-01

    Hemocyanin is an important multifunctional non-specific immune molecule. In this study, we purified lectin binding and non-lectin binding hemocyanin from Litopenaeus vannamei using Concanavalin A (ConA) lectin affinity chromatography (designated HMC-C and HMC-NC, respectively). Analysis of the carbohydrate content showed that HMC-C had about 20 times as much carbohydrate as HMC-NC. 54 and 42 peaks were observed in HMC-C and HMC-NC by HPLC, which reduced to 49 and 6 peaks, respectively, when digested with trypsin and repurified with ConA lectin column. Further, the agglutinative activity of HMC-C against two pathogenic bacteria, Vibrio alginolyticus and Vibrio fluvialis, was about 8-fold and 4-fold, respectively, to that of HMC-NC. While the antibacterial activity of HMC-NC was about 30% lower compared with HMC-C. Similarly, when HMC was deglycosylated using O-glycosidase, its agglutinative activity reduced about 4-8 fold. Most importantly, when shrimps were challenged with V. alginolyticus or V. fluvialis, the glycan content of hemocyanin increased dramatically and remained high at the earlier time points (24-72h) post infection, only decreasing after 96 hpi. Taken together, these results suggest that hemocyanin glycosylation plays an important role in its antibacterial properties. Copyright © 2017 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

  11. Standardization of sodium metabisulfite solution concentrations and immersion time for farmed shrimp Litopenaeus vannamei

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    Luciana Trigueiro de Andrade

    2015-03-01

    Full Text Available Sodium metabisulfite is the main additive used in the prevention of melanosis in shrimp; however, it has currently been employed with great variation in concentration by producers. Thus, the aim of the present study was to determine the correlation between the concentration of the sodium metabisulfite solution and immersion time of the whole shrimp to obtain the concentration of sulfur dioxide (SO2 in the edible muscle of farmed shrimp (Litopenaeus vannamei in accordance with the limit established by law. For this, solutions of sodium metabisulfite at different concentrations (1%, 2 %, 3 %, 4% and 5% were prepared and samples of L. vannamei shrimp (100g were immersed during 10, 20 or 30 minutes at temperature of 7°C. For all treatment assayed the concentration of SO2 was determined in the edible muscle of farmed shrimp (L. vannamei. The results show that for the conditions used in this study, the correlations were linear, with significant increase (P<0.05 in the SO2 concentration in the edible muscle of shrimps both increasing sodium metabisulfite concentration as increasing immersion times, suggesting the immersion of shrimps in a 3% solution for a time of 13 minutes in order to obtain SO2 concentration of 100ppm in its edible muscle in accordance with Brazilian legislation

  12. Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil

    Directory of Open Access Journals (Sweden)

    Ligia Maria Rodrigues de Melo

    2011-12-01

    Full Text Available Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50% presented multiple antibiotic resistance to ampicillin (90% and amikacin (60%, while two strains (20% displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90%, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range: 0.14-0.29, indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.

  13. Structural insights from a novel invertebrate triosephosphate isomerase from Litopenaeus vannamei

    Science.gov (United States)

    Lopez-Zavala, Alonso A.; Carrasco-Miranda, Jesus S.; Ramirez-Aguirre, Claudia D.; López-Hidalgo, Marisol; Benitez-Cardoza, Claudia G.; Ochoa-Leyva, Adrian; Cardona-Felix, Cesar S.; Diaz-Quezada, Corina; Rudiño-Piñera, Enrique; Sotelo-Mundo, Rogerio R.; Brieba, Luis G.

    2016-01-01

    Triosephosphate isomerase (TIM; EC 5.3.1.1) is a key enzyme involved in glycolysis and gluconeogenesis. Glycolysis is one of the most regulated metabolic pathways, however little is known about the structural mechanisms for its regulation in non-model organisms, like crustaceans. To understand the structure and function of this enzyme in invertebrates, we obtained the crystal structure of triosephosphate isomerase from the marine Pacific whiteleg shrimp (Litopenaeus vannamei, LvTIM) in complex with its inhibitor 2-phosphogyceric acid (2-PG) at 1.7 Å resolution. LvTIM assembles as a homodimer with residues 166-176 covering the active site and residue Glu166 interacting with the inhibitor. We found that LvTIM is the least stable TIM characterized to date, with the lowest range of melting temperatures, and with the lowest activation enthalpy associated with the thermal unfolding process reported. In TIMs dimer stabilization is maintained by an interaction of loop 3 by a set of hydrophobic contacts between subunits. Within these contacts, the side chain of a hydrophobic residue of one subunit fits into a cavity created by a set of hydrophobic residues in the neighboring subunit, via a "ball and socket" interaction. LvTIM presents a Cys47 at the "ball" inter-subunit contact indicating that the character of this residue is responsible for the decrease in dimer stability. Mutational studies show that this residue plays a role in dimer stability but is not a solely determinant for dimer formation. PMID:27614148

  14. Beneficial effects of Ectothiorhodospira shaposhnikovii WF on larval cultivation of Litopenaeus vannamei.

    Science.gov (United States)

    Wen, C Q; Xue, M; Liang, H F; Wu, Y; Li, X

    2015-01-01

    To develop high quality probiotics for shrimp larviculture, the effects of a photosynthetic purple sulphur bacterium WF identified as Ectothiorhodospira shaposhnikovii on survival and development of Litopenaeus vannamei larvae were evaluated in vivo. The larvae exhibited a better survival rate after administration of strain WF compared to the probiotic Rhodopseudomonas palustris. To investigate the effect of dose and dosing frequency, strain WF was added to larvae, stages nauplius 6 to zoea 3, at three different doses and dosing frequencies. Larval treatment with strain WF twice at 10(6) cfu/ml exhibited significantly higher survival compared to the other doses and dosing frequencies as well as the control. The effect on water quality was assessed by applying strain WF to larvae, stages nauplius 6 to postlarvae 1, under conditions of zero water exchange and one-third water exchange. The larvae exhibited higher survival and faster growth when treated under conditions of zero water exchange. No significant difference was detected in the levels of three water quality parameters and in vibrio counts between these two conditions. Therefore, E. shaposhnikovii WF acts both as a bioremediation agent and nutrient source and can benefit shrimp larvae if given at an appropriate dose and dosing frequency. Strain WF, a moderate halophile, shows great promise as a water additive in improving water quality and providing nutrition for shrimp larviculture.

  15. Encapsulated Synbiotic Dietary Supplementation at Different Dosages to Prevent Vibriosis in White Shrimp, Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Anis Zubaidah

    2015-10-01

    Full Text Available The aim of this study was to evaluate the effect of encapsulated synbiotic (Bacillus sp. NP5 and oligosaccharide dietary at different dosages on growth performance, survival rate, feed conversion ratio, and immune responses of Litopenaeus vannamei against Vibrio infection. The shrimps of the main treatments were fed by the diet that contained three different dosages of encapsulated synbiotic [0.5% (A, 1% (B, and 2% (C (w/w] with feeding rate of 5% of shrimp biomass (4 times a day. The shrimps of two control treatments (negative control and positive control were fed only by commercial feed without supplementation of encapsulated synbiotic. The growth, feed conversion ratio, and survival rate were observed after 30 days of encapsulated synbiotic dietary. The shrimps were then challenged by injection of Vibrio harveyi (6 log colony forming units/mL 0.1 mL/shrimp, excluded the negative control treatment. Afterward, the survival and immune responses were observed for 9 days after experimental infection. The shrimps treated with 2% encapsulated synbiotic (treatment C in the diet showed the highest growth performance (2.98 ± 0.42%, feed conversion ratio (1.26 ± 0.19, and better immune responses i.e. total hemocyte counts, differential hemocyte count, phenoloxidase, and intestine bacteria observation compared to those of positive control treatment.

  16. Nitrogen dynamics model in zero water exchange, low salinity intensive ponds of white shrimp, Litopenaeus vannamei, at Colima, Mexico

    Directory of Open Access Journals (Sweden)

    Francisco A Castillo-Soriano

    2013-03-01

    Full Text Available We present a mathematical model based on differential equations describing the dynamics of nitrogen (NH4+, NO2-, NO3- and organic nitrogen in phytoplankton in ponds of white shrimp (Litopenaeus vannamei, with low salinity and zero turnovers, from planting to harvest. The model predicts the results of commercial production in three ponds. We show that this culture system, without replacement, retains the nitrogen and shrimp produced a lower feed conversion in comparison with systems with replacement. The model can be used to define strategies for improved performance.

  17. Growth and metabolic responses of whiteleg shrimp Litopenaeus vannamei and Nile tilapia Oreochromis niloticus in polyculture fed with potential probiotic microorganisms on different schedules: Crecimiento y respuesta metabólica del camarón blanco Litopenaeus vannamei y tilapia del Nilo Oreochromis niloticus en policultivo alimentado con microorganismos probióticos potenciales en diferentes frecuencias

    Directory of Open Access Journals (Sweden)

    Juan P Apún-Molina

    2015-07-01

    Full Text Available Probiotics and co-culture of shrimp and tilapia are two strategies to improve yield and eco-efficiency of both species. However, only few studies have analyzed the combination of these two strategies. This study analyzes the effect of a mixture of potential probiotics supplied in the diet at different frequencies in a system of shrimp (10 m-2 and tilapia (5 m-2 in a trial lasting 84 days. The probiotics mixture was composed of four lactic acid bacteria and one yeast strain that were supplied either daily, every 5 days, or every 10 days in the diet and compared with a control without any supply of probiotics. At the end of the experiment, blood chemistry and hematology of shrimp and tilapia were analyzed as an index of physiological condition. Water quality did not differ between treatments. The final weight, feed conversion ratio, and yield of shrimp were significantly higher with daily supply of probiotics compared to shrimp that did not receive probiotics supply, with intermediate values for treatments with probiotics supply every 5 or 10 days. However, level of supplementation every 5 days could be considered as optimal because no significant differences with daily supply were observed for any variable, whereas the supply each 10 days resulted in a significantly lower yield. Significantly higher final weight, feed conversion ratio, and yield of tilapia occurred with daily supplements. Blood cholesterol in tilapia significantly decreased with increasing frequency of probiotics supplementation. These results indicate that probiotics supplements for shrimp and tilapia cultivated simultaneously improved yields and physiological condition.

  18. Production and valuation of foods for animal monogástricos, with biological silage of remains of the prawn prosecution (Litopenaeus vannamei) fermented with lactobacillus

    OpenAIRE

    Sánchez, Héctor; Ochoa, Gloria

    2016-01-01

    This study allowed the valuation of diets using biological silage of residuals of the prosecution of (Litopenaeus vannamei) EB, fermented inoculum of commercial organisms (Lactobacillos acidofilos) or isolated from the digestive tract of the pig (Enterococcus hirae, Lactobacillus brevis, Lactobacillus johnsonii, Pediococcus pentosaceus) single use in piglets; EB preparation, cooking residues, milling, mixing (molasses and inoculum) and incubation; diet formulation according sp. and physiologi...

  19. Biología de los camarones Peneidos del manglar de Guadalupe

    NARCIS (Netherlands)

    Rojas-Beltrán, Ricardo

    1981-01-01

    Durante el desarrollo de dos campañas preliminares en 1976 hemos podido recolectar ocho especies de Peneidos en el manglar de Guadalupe. Las citaremos en orden de importancia: Penaeus (Melicerlus) aztecus subtilis, P. (M.) brasiliensis, P. (M.) duorarum notialis, P. (Litopenaeus) schmitti,

  20. Polyphenolic glycosides isolated from Pogostemon cablin (Blanco) Benth. as novel influenza neuraminidase inhibitors.

    Science.gov (United States)

    Liu, Fang; Cao, Wei; Deng, Chao; Wu, Zhaoquan; Zeng, Guangyao; Zhou, Yingjun

    2016-01-01

    Influenza is historically an ancient disease that causes annual epidemics and, at irregular intervals, pandemics. At present, the first-line drugs (oseltamivir and zanamivir) don't seem to be optimistic due to the spontaneously arising and spreading of oseltamivir resistance among influenza virus. Pogostemon cablin (Blanco) Benth. (P. cablin) is an important traditional Chinese medicine herb that has been widely used for treatment on common cold, nausea and fever. In our previous study, we have identified an extract derived from P. cablin as a novel selective neuraminidase (NA) inhibitor. A series of polyphenolic compounds were isolated from P. cablin for their potential ability to inhibit neuraminidase of influenza A virus. Two new octaketides (1, 2), together with other twenty compounds were isolated from P. cablin. These compounds showed better inhibitory activity against NA. The significant potent compounds of this series were compounds 2 (IC50 = 3.87 ± 0.19 μ mol/ml), 11, 12, 14, 15, 19 and 20 (IC50 was in 2.12 to 3.87 μ mol/ml), which were about fourfold to doubled less potent than zanamivir and could be used to design novel influenza NA inhibitors, especially compound 2, that exhibit increased activity based on these compounds. With the help of molecular docking, we had a preliminary understanding of the mechanism of the two new compounds (1-2)' NA inhibitory activity. Fractions 6 and polyphenolic compounds isolated from fractions 6 showed higher NA inhibition than that of the initial plant exacts. The findings of this study indicate that polyphenolic compounds and fractions 6 derived from P. cablin are potential NA inhibitors. This work is one of the evidence that P. cablin has better inhibitory activity against influenza, which not only enriches the compound library of P. cablin, but also facilitates further development and promises its therapeutic potential for the rising challenge of influenza diseases.

  1. Assessment of hydrologic transport of radionuclides from the Rio Blanco underground nuclear test site, Colorado

    International Nuclear Information System (INIS)

    Chapman, J.; Earman, S.; Andricevic, R.

    1996-10-01

    DOE is operating an environmental restoration program to characterize, remediate, and close non-Nevada Test Site locations used for nuclear testing. Evaluation of radionuclide transport by groundwater is part of preliminary risk analysis. These evaluations allow prioritization of test areas in terms of risk, provide a basis for discussions with regulators and the public about future work, and provide a framework for assessing site characterization data needs. The Rio Blanco site in Colorado was the location of the simultaneous detonation of three 30-kiloton nuclear devices. The devices were located 1780, 1899, and 2039 below ground surface in the Fort Union and Mesaverde formations. Although all the bedrock formations at the site are thought to contain water, those below the Green River Formation (below 1000 in depth) are also gas-bearing, and have very low permeabilities. The transport scenario evaluated was the migration of radionuclides from the blast-created cavity through the Fort Union Formation. Transport calculations were performed using the solute flux method, with input based on the limited data available for the site. Model results suggest that radionuclides from the test are contained entirely within the area currently administered by DOE. This modeling was performed to investigate how the uncertainty in various physical parameters affect radionuclide transport at the site, and to serve as a starting point for discussion regarding further investigation; it was not intended to be a definitive simulation of migration pathways or radionuclide concentration values. Given the sparse data, the modeling results may differ significantly from reality. Confidence in transport predictions can be increased by obtaining more site data, including the amount of radionuclides which would have been available for transport (i.e., not trapped in melt glass or vented during gas flow testing), and the hydraulic properties of the formation. 38 refs., 6 figs., 1 tab

  2. The effect of phytase levels in the diet on the digestibility and growth performance of white shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Muhammmad Agus Suprayudi

    2013-11-01

    Full Text Available This experiment was conducted to evaluate the effect of phytase levels in the diet on the digestibility and growth performance of white shrimp Litopenaeus vanname. White shrimp were used in the trial with initial body weight of 3.8±0.01 g were reared in  aquarium (60×40×50 cm3 and filled with 60L treated sea water at a density of ten individu. Experimental diet were formulated in isonitrogeneous (36% protein and isocaloric (3960 kcal/kg to contain four levels of phytase: 0 iu/kg (diet A, 500 iu/kg (diet B, 750 iu/kg (diet C, and 1000 iu/kg(diet D. All diets were supplied with 0.5% of Cr2O3 as an indicator for digestibility measurement shrimp fed three times daily at satiation levelfor how many days of rearing. A factorial completely randomized experimental design was selected consisted of four treatments and triplicates. The result showed that the phytase addition improved the digestibility of protein, calcium, and phosphor as well as improve growth performance of white shrimp. Based on evaluation of those parameters, it is concluded that phytase level of500 IU support the best growth performance ofjuvenile white shrimp. Keywords: phytase, feed digestibility, growth,Litopenaeus vannamei

  3. FONTES E NÍVEIS DE VITAMINA C PARA O CAMARÃO Litopenaeus vannamei NA FASE DE RECRIA

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    Neyva Ribeiro Ferraz

    2012-09-01

    Full Text Available The inclusion of different sources and levels of vitamin C was evaluated on growth, survival, protein retention and health of Litopenaeus vannamei with initial weight of 5.90 ± 0.57 g. Five hundred and forty individuals were distributed in 27 cages in a completely randomized design in a 4x2 factorial scheme (four sources and two levels (n = 3. The sources used were ascorbic acid crystal, coated ascorbic acid, ascorbic acid monophosphate and ascorbic acid PEG (polyethylene glycol with two levels: 180 and 260 mg kg-1. No influence was observed (P>0.05 from the source, level of vitamin C nor interaction of these factors on growth, survival, retention of protein and parameters of health of the shrimp. Necrosis was observed in the abdomen of all animals. The use of vitamin C in sources and levels estimated for the shrimp Litopenaeus vannamei weighing over 5 g shows no improvement in growth, survival, protein retention and health of shrimp.

  4. Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system.

    Science.gov (United States)

    Cardona, Emilie; Gueguen, Yannick; Magré, Kevin; Lorgeoux, Bénédicte; Piquemal, David; Pierrat, Fabien; Noguier, Florian; Saulnier, Denis

    2016-07-19

    Biofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology. Analyses of the bacterial community identified in water from BFT and "clear seawater" (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments. This study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.

  5. Effect of bioactive peptides (BPs) on the development of Pacific white shrimp ( Litopenaeus vannamei Boone, 1931)

    Science.gov (United States)

    Wang, Guangjun; Yu, Ermeng; Li, Zhifei; Yu, Deguang; Wang, Haiying; Gong, Wangbao

    2016-06-01

    The present study was conducted to evaluate the feasibility of replacing fish meal (FM) with bioactive peptides (BPs) in diet of white shrimp ( Litopenaeus vannamei). The changes in growth performance, body composition, non-specific immunity, and water quality were examined after the shrimp were fed four diets, in which 0% (control), 33.3%, 66.7% and 100% of FM was replaced by BPs, respectively. The groups were designated as Con, 1/3BPs, 2/3BPs, and 3/3BPs. A total of 720 shrimp with an initial body weight of 1.46 ± 0.78 g were fed the experimental diets for 56 days. The results revealed that: 1) the weight gain rate (WGR) in 1/3BPs, 2/3BPs, and 3/3BPs was significantly higher than that in Con ( P < 0.05), while no significant difference was found on survival rate and feed conversion ratio (FCR); 2) the whole-body crude protein (CP) and crude lipids (CL) were significantly different among groups, while there was no significant difference between crude ash and phosphorus contents; 3) the levels of acid phosphatase (ACP), lysozyme (LZM), superoxide dismutase (SOD), phenol oxidase (PO) and bactericidal activity increased significantly with the inclusion of BPs; 4) in terms of water quality, no significant difference was found in pH and dissolved oxygen among diets during the whole experimental period. Moreover, even though nitrite and ammonium levels tended to increase with time, there was no significant difference among groups. The results indicated that BPs is an applicable alternative of protein source, which can substitute FM in the diets of L. vannamei; it is able to effectively promote growth performance and improve immunity. Moreover, BPs in the diets had no negative impact on water quality.

  6. Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress

    Science.gov (United States)

    Lu, Xia; Kong, Jie; Luan, Sheng; Dai, Ping; Meng, Xianhong; Cao, Baoxiang; Luo, Kun

    2016-01-01

    In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which usually brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens, reducing growth, decreasing osmoregulatory capacity, increasing the molting frequency, and even causing high mortality. However, little information is available on the molecular mechanisms of the detrimental effects of ammonia stress in shrimp. In this study, we performed comparative transcriptome analysis between ammonia-challenged and control groups from the same family of L. vannamei to identify the key genes and pathways response to ammonia stress. The comparative transcriptome analysis identified 136 significantly differentially expressed genes that have high homologies with the known proteins in aquatic species, among which 94 genes are reported potentially related to immune function, and the rest of the genes are involved in apoptosis, growth, molting, and osmoregulation. Fourteen GO terms and 6 KEGG pathways were identified to be significantly changed by ammonia stress. In these GO terms, 13 genes have been studied in aquatic species, and 11 of them were reported potentially involved in immune defense and two genes were related to molting. In the significantly changed KEGG pathways, all the 7 significantly changed genes have been reported in shrimp, and four of them were potentially involved in immune defense and the other three were related to molting, defending toxicity, and osmoregulation, respectively. In addition, majority of the significantly changed genes involved in nitrogen metabolisms that play an important role in reducing ammonia toxicity failed to perform the protection function. The present results have supplied molecular level support for the previous founding of the detrimental effects of ammonia stress in shrimp, which is a prerequisite for better understanding the molecular

  7. Distribution of trace elements in tissues of shrimp species Litopenaeus vannamei (Boone, 1931 from Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    E. Silva

    Full Text Available Abstract In this study, concentrations of trace elements in tissues of shrimp species (Litopenaeus vannamei from farming and zone natural coastal located in the northeastern Brazil were investigated. The elements determination was performed by optical emission spectrometry with inductively coupled plasma (ICP OES. The following ranges of concentrations in the tissues were obtained in µg g–1 dry weight: Al: 13.4-886.5, Cd: 0.93-1.80; Cu: 24.8-152; Fe: 3.2-410.9; Mn: 0.36-24.4; Se: 0.094-9.81 and Zn: 20.3-109.4. The shrimp muscle can be a good iron source (about 88.9 mg–1g dry weight. The distribution of Se concentration in tissues showed much variation between locations, and the concentration levels found in shrimp muscles of wild samples were high, where its levels in 67% of muscle and 50% of others tissues samples exceeded the ANVISA limit, indicating evidence of selenium bioaccumulation. Significant correlation was observed between the following pairs of elements: Fe-Zn (r= –0.70, Mn-Cu (r= –0.74, Se-Cu (r= –0.68, Se-Mn (r= 0.82 in the muscles; Fe-Al (r= 0.99, Mn-Al (r= 0.62, Mn-Fe (r= 0.62, Se-Al (r = 0.88, Se-Fe (r= 0.87, Se-Mn (r= 0.58 in the exoskeleton and Cu-Zn (r = 0.68, Al-Cu (r= 0.88, Fe-Cu (r= 0.95 and Fe-Al (r= 0.97 in the viscera.

  8. The upregulation of immune responses in tyrosine hydroxylase (TH) silenced Litopenaeus vannamei.

    Science.gov (United States)

    Mapanao, Ratchaneegorn; Chang, Chin-Chyuan; Cheng, Winton

    2017-02-01

    Catecholamines (CAs) play a crucial role in maintaining physiological and immune homeostasis in invertebrates and vertebrates under stressful conditions. Tyrosine hydroxylase (TH) is the first and rate-limiting enzyme in CA synthesis. To develop an effective CA-related immunological defense system against stress and pathogen infection, various criteria, were evaluated in TH double-stranded (ds) RNA-injected white shrimp, Litopenaeus vannamei. Specifically, the relative transcript quantification of TH, dopamine β-hydroxylase (DBH), crustacean hyperglycemic hormone (CHH), and other immune-related genes; TH activity in the haemolymph; and the estimation of l-dihydroxyphenylalanine (l-DOPA), glucose, and lactate levels in the haemolymph were examined. TH depletion revealed a significant increase in the total haemocyte count; granular cells; semigranular cells; respiratory bursts (RBs, release of superoxide anion); superoxide dismutase (SOD) activity; phagocytic activity and clearance efficiency; and the expression of lipopolysaccharide and β-1,3-glucan-binding protein and peroxinectin, SOD, crustin, and lysozyme genes. In addition, the reduction of TH gene expression and activity was accompanied by a decline of phenoloxidase (PO) activity per granulocyte, lower glucose and lactate levels, and significantly low expression of DBH and CHH genes. However, the number of hyaline cells, activity of PO, RBs per haemocyte, and expression of POI and POII genes were not significantly different in the LvTH-silenced shrimp. Notably, the survival ratio of LvTH-silenced shrimp was significantly higher than that of shrimp injected with diethyl pyrocarbonate-water and nontargeting dsRNA when challenged with Vibrio alginolyticus. Therefore, the depletion of TH can enhance disease resistance in shrimp by upregulating specific immune parameters but downregulating the levels of carbohydrate metabolites. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Evaluation of coexposure to inorganic arsenic and titanium dioxide nanoparticles in the marine shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Cordeiro, Lucas; Müller, Larissa; Gelesky, Marcos A; Wasielesky, Wilson; Fattorini, Daniele; Regoli, Francesco; Monserrat, José Marìa; Ventura-Lima, Juliane

    2016-01-01

    The acute toxicity of titanium dioxide nanoparticles (nTiO2) that occur concomitantly in the aquatic environment with other contaminants such as arsenic (As) is little known in crustaceans. The objective of the present study is to evaluate whether coexposure to nTiO2 can influence the accumulation, metabolism, and oxidative stress parameters induced by arsenic exposure in the gills and hepatopancreas of the shrimp Litopenaeus vannamei. Organisms were exposed by dissolving chemicals in seawater (salinity = 30) at nominal concentrations of 10 μg/L nTiO2 or As(III), dosed alone and in combination. Results showed that there was not a significant accumulation of As in either tissue type, but the coexposure altered the pattern of the metabolism. In the hepatopancreas, no changes were observed in the biochemical response, while in the gills, an increase in the glutamate-cysteine-ligase (GCL) activity was observed upon exposure to As or nTiO2 alone, an increase in the reduced glutathione (GSH) levels was observed upon exposure to As alone, and an increase in the total antioxidant capacity was observed upon exposure to nTiO2 or nTiO2 + As. However, these modulations were not sufficient enough to prevent the lipid damage induced by nTiO2 exposure. Our results suggest that coexposure to nTiO2 and As does not alter the toxicity of this metalloid in the gills and hepatopancreas of L. vannamei but does alter its metabolism, favoring its accumulation of organic As species considered moderately toxic.

  10. Selection for growth performance of tank-reared Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai

    2013-05-01

    Seven growth-related traits were measured to assess the selection response and genetic parameters of the growth of Pacific white shrimp, Litopenaeus vannamei, which had been domesticated in tanks for more than four generations. Phenotypic and genetic parameters were evaluated and fitted to an animal model. Realized response was measured from the difference between the mean growth rates of selected and control families. Realized heritability was determined from the ratio of the selection responses and selection differentials. The animal model heritability estimate over generations was 0.44±0.09 for body weight (BW), and ranged from 0.21±0.08 to 0.37±0.06 for size traits. Genetic correlations of phenotypic traits were more variable (0.51-0.97), although correlations among various traits were high (>0.83). Across generations, BW and size traits increased, while selection response and heritability gradually decreased. Selection responses were 12.28%-23.35% for harvest weight and 3.58%-13.53% for size traits. Heritability estimates ranged from 0.34±0.09 to 0.48±0.15 for harvest weight and 0.17±0.01-0.38±0.11 for size traits. All phenotypic and genetic parameters differed between various treatments. To conclude, the results demonstrated a potential for mass selection of growth traits in L. vannamei. A breeding scheme could use this information to integrate the effectiveness constituent traits into an index to achieve genetic progress.

  11. Mercury content and their risk assessment in farmed shrimp Litopenaeus vannamei from NW Mexico.

    Science.gov (United States)

    Delgado-Alvarez, C G; Ruelas-Inzunza, J; Osuna-López, J I; Voltolina, D; Frías-Espericueta, M G

    2015-01-01

    The main objective of this study was to evaluate the total mercury content in hepatopancreas and edible muscle of the whiteleg shrimp Litopenaeus vannamei cultured along the NW coast of Mexico, and to evaluate the potential human health risk due to their consumption. Samples were obtained between May and June 2010 in 26 shrimp farms from the three most important shrimp-producing states of NW Mexico, and total Hg was analyzed after reduction with SnCl2 in a mercury analyzer. The ranges of Hg concentrations of the hepatopancreas were 0.101±0.03-0.184±0.13 μg g(-1) in Sonora, 0.077±0.055-0.813±0.363 μg g(-1) in Sinaloa and 0.139±0.037-0.791±0.33 μg g(-1) in Nayarit. In the muscle, values were from 0.078±0.02 to 0.539±0.09 μg g(-1) in Sonora, 0.154±0.03-0.861±0.423 μg g(-1) in Sinaloa and 0.121±0.041-1.48±0.44 μg g(-1) in Nayarit. Considering the concentrations of Hg in the muscle and the national consumption rate, shrimp farmed in NW Mexico does not represent a risk for human health (HQ<1). Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Biochemical changes of Litopenaeus vannamei and Fenneropenaeus indicus in the different stages of WSSV infection

    Directory of Open Access Journals (Sweden)

    Ramachandran Shalini

    2013-01-01

    Full Text Available Objective: To find out the difference in the proximate composition and fatty acid profile of both the species of shrimp Litopenaeus vannamei (L. vannamei and Fenneropenaeus indicus (F. indicus infected with different stages of white spot syndrome virus (WSSV. Methods: Standard methods were followed by estimating the proximate composition and fatty acid analysis. Each fish specimens were beheaded, eviscerated and filleted manually. The tissue samples were oven dried at 67 °C for 24 h. Then the samples were grounded finely with pestle and mortar. The saponified samples were cooled at room temperature for 25 min. They were acidified and methylated by adding 2 mL 54% 6 mol/L HCL in 46% aqueous methanol and incubated at 80 °C for 10 min in water bath. Following the base wash step, the fatty acid methyl esters were cleaned in anhydrous sodium sulphate and then transferred into gas chromatograph sample vial for analysis. Fatty acid methyl esters were separated by gas chromatograph. Results: The proximate composition was higher in the both control tissue than the three (low, moderate, severe infected ones. For L. vannamei and F. indicus, the carbohydrates are 5.07% and 6.18%, and the proteins are 25.01% and 22.17%, respectively. Lipid level recorded was little higher in the shrimps maintained and showed severe sign of WSSV infection than the control and the fatty acid profile result revealed that saturated fatty acids and monounsaturated fatty acid was in higher [48.72% (Severe & 16.87% (low] L. vannamei. In the polyunsaturated fatty acid, F. indicus was 40.47% (low. Conclusions: Our study showed that the healthy shrimps are nutritionally rich than the WSSV affected shrimps.

  13. Biochemical changes of Litopenaeus vannamei and Fenneropenaeus indicus in the different stages of WSSV infection

    Directory of Open Access Journals (Sweden)

    Ramachandran Shalini

    2013-08-01

    Full Text Available Objective: To find out the difference in the proximate composition and fatty acid profile of both the species of shrimp Litopenaeus vannamei (L. vannamei and Fenneropenaeus indicus (F. indicus infected with different stages of white spot syndrome virus (WSSV. Methods: Standard methods were followed by estimating the proximate composition and fatty acid analysis. Each fish specimens were beheaded, eviscerated and filleted manually. The tissue samples were oven dried at 67 °C for 24 h. Then the samples were grounded finely with pestle and mortar. The saponified samples were cooled at room temperature for 25 min. They were acidified and methylated by adding 2 mL 54% 6 mol/L HCL in 46% aqueous methanol and incubated at 80 °C for 10 min in water bath. Following the base wash step, the fatty acid methyl esters were cleaned in anhydrous sodium sulphate and then transferred into gas chromatograph sample vial for analysis. Fatty acid methyl esters were separated by gas chromatograph. Results: The proximate composition was higher in the both control tissue than the three (low, moderate, severe infected ones. For L. vannamei and F. indicus, the carbohydrates are 5.07% and 6.18%, and the proteins are 25.01% and 22.17%, respectively. Lipid level recorded was little higher in the shrimps maintained and showed severe sign of WSSV infection than the control and the fatty acid profile result revealed that saturated fatty acids and monounsaturated fatty acid was in higher [48.72% (Severe & 16.87% (low] L. vannamei. In the polyunsaturated fatty acid, F. indicus was 40.47% (low. Conclusions: Our study showed that the healthy shrimps are nutritionally rich than the WSSV affected shrimps.

  14. Identification of cold responsive genes in Pacific white shrimp (Litopenaeus vannamei) by suppression subtractive hybridization.

    Science.gov (United States)

    Peng, Jinxia; Wei, Pinyuan; Chen, Xiuli; Zeng, Digang; Chen, Xiaohan

    2016-01-10

    The Pacific white shrimp (Litopenaeus vannamei) is one of the most widely cultured shrimp species in the world. Despite L. vannamei having tropical origins, it is being reared subtropically, with low temperature stress being one of the most severe threats to its growth, survival and distribution. To unravel the molecular basis of cold tolerance in L. vannamei, the suppression subtractive hybridization (SSH) platform was employed to identify cold responsive genes in the hepatopancreas of L. vannamei. Both forward and reverse cDNA libraries were constructed, followed by dot blot hybridization, cloning, sequence analysis and quantitative real-time PCR. These approaches identified 92 cold induced and 48 cold inhibited ESTs to give a total of 37 cold induced and 17 cold inhibited contigs. Some of the identified genes related to stress response or cell defense, such as tetraspanins (TSPANs), DEAD-box helicase, heat shock proteins (HSPs) and metallothionein (MT), which were more abundant in the forward SSH library than in the reverse SSH library. The most abundant Est was a tetraspanin-8 (TSPAN8) homolog dubbed LvTSPAN8. A multiple sequence alignment and transmembrane domain prediction was also performed for LvTSPAN8. LvTSPAN8 expression was also examined in the gills, muscle, heart and hepatopancreas following cold exposure and showed the highest expression levels in the hepatopancreas. Overall, this study was able to identify several known genes and novel genes via SSH that appear to be associated with cold stress and will help to provide further insights into the molecular mechanisms regulating cold tolerance in L. vannamei. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Caracterización agronómica una accesión de Swinglea glutinosa (Blanco) Merr, (Rutaceae)

    OpenAIRE

    Rafael Jiménez Villasuso; Fressy Pérez Campo; Marta Rosa Hernández Zaldívar; Víctor Ramón Fuentes Fiallo; Josefa Bárbara Velazquez Palenzuela

    2015-01-01

    RESUMEN La familia Rutaceae abarca un gran número de géneros y especies. De ellos, Citrus, Poncirus y Fortunella son los géneros más explotados comercialmente a nivel mundial, sin embargo, hay otros menos abordados, como el género Swinglea que posee una sola especie: S. glutinosa (Blanco) Merr. Con el objetivo de caracterizar desde el punto de vista agronómico esta especie por su de importancia como recurso genético y posterior utilización como patrón en la producción citrícola. Se evalua...

  16. Crecimiento en pastoreo rotacional de toretes de razas criollas Romosinuano y Blanco Orejinegro en Colombia

    Directory of Open Access Journals (Sweden)

    Jaime Quiceno A.

    2012-04-01

    Full Text Available Objetivo. Evaluar el crecimiento de toretes de las razas Romosinuno (ROMO y Blanco Orejinegro (BON en una prueba de comportamiento en pastoreo rotacional. Materiales y métodos. La prueba fue desarrollada en la Estación Experimental El Nus, en la Región Andina Colombiana, donde se evaluaron 20 toretes BON y 16 ROMO provenientes de quince ganaderías comerciales, los cuales fueron mantenidos en un solo grupo durante 221 días en pastoreo rotacional en franjas con periodos cortos de ocupación, de los cuales 56 días fueron en pastoreo no suplementado y 165 días bajo tres diferentes fases de pastoreo suplementado en el potrero. Se realizaron pesajes cada 28 días y se evaluaron variables como: la evolución en peso, la ganancia total y diaria, la diferencia entre pesajes, la relación consumo peso vivo y la tasa de consumo. Resultados. El peso inicial en la raza BON fue 180.4±36.9 kilos con 9.6±1.74 meses de edad y en la raza ROMO fue de 171.8±32.6 kilos con 10.1±3.2 meses de edad. El incremento general de los individuos entre pesajes fue 0.497 kilos por día para los individuos de raza BON, y 0.366 kilos por día para la raza ROMO. En la prueba de eficiencia la tasa de consumo de suplemento alcanzada fue 64.8% y 71% para BON y ROMO respectivamente, equivalentes a una ingestión de materia seca de 0.47% y 0.53% con relación al peso vivo. Conclusiones. Este trabajo evidencia un mayor desempeño de los individuos de la raza BON comparados con los animales de la raza ROMO e indica una alta variabilidad en la respuesta a un manejo semi-intensivo en las poblaciones en evaluación.

  17. Metalliferous sediment and a silica-hematite deposit within the Blanco fracture zone, Northeast Pacific

    Science.gov (United States)

    Hein, J.R.; Clague, D.A.; Koski, R.A.; Embley, R.W.; Dunham, R.E.

    2008-01-01

    A Tiburon ROV dive within the East Blanco Depression (EBD) increased the mapped extent of a known hydrothermal field by an order of magnitude. In addition, a unique opal-CT (cristobalite-tridymite)-hematite mound was discovered, and mineralized sediments and rock were collected and analyzed. Silica-hematite mounds have not previously been found on the deep ocean floor. The light-weight rock of the porous mound consists predominantly of opal-CT and hematite filaments, rods, and strands, and averages 77.8% SiO2 and 11.8% Fe2O3. The hematite and opal-CT precipitated from a low-temperature (???115?? C), strongly oxidized, silica- and iron-rich, sulfur-poor hydrothermal fluid; a bacterial mat provided the framework for precipitation. Samples collected from a volcaniclastic rock outcrop consist primarily of quartz with lesser plagioclase, smectite, pyroxene, and sulfides; SiO2 content averages 72.5%. Formation of these quartz-rich samples is best explained by cooling in an up-flow zone of silica-rich hydrothermal fluids within a low permeability system. Opal-A, opal-CT, and quartz mineralization found in different places within the EBD hydrothermal field likely reflects decreasing silica saturation and increasing temperature of the mineralizing fluid with increasing silica crystallinity. Six push cores recovered gravel, coarse sand, and mud mineralized variously by Fe or Mn oxides, silica, and sulfides. Total rare-earth element concentrations are low for both the rock and push core samples. Ce and Eu anomalies reflect high and low temperature hydrothermal components and detrital phases. A remarkable variety of types of mineralization occur within the EBD field, yet a consistent suite of elements is enriched (relative to basalt and unmineralized cores) in all samples analyzed: Ag, Au, S, Mo, Hg, As, Sb, Sr, and U; most samples are also enriched in Cu, Pb, Cd, and Zn. On the basis of these element enrichments, the EBD hydrothermal field might best be described as a base

  18. Estudio de la co-infección viral ihhnv-wssv en el camarón blanco l. vannamei

    OpenAIRE

    Melena, José

    2005-01-01

    Estudio de la Co-infección viral IHHNV-WSSV en el camarón blanco L. vannamei En Ecuador, el desarrollo y subsistencia de la industria dedicada al cultivo del camarón blanco L. vannamei han sido amenazados por la aparición de patógenos virales, siendo los de mayor importancia el Virus de la Necrosis infecciosa hipodérmica y hematopoyética (IHHNV) y el Virus del Síndrome de la mancha blanca (WSSV). El IHHNV no causa la muerte de L. vannamei, sino que produce una enfermedad llamada Runt...

  19. Rendimiento del pasto ballico perenne (Lolium perenne L.) y ovillo (Dactylis glomerata L.) solos y asociados con trébol blanco (Trifolium repens L.).

    OpenAIRE

    Mendoza Pedroza, Sergio Iban

    2014-01-01

    El objetivo del estudio fue evaluar la respuesta productiva de los pastos ballico perenne y ovillo solos y asociados con trébol blanco en las siguientes proporciones: 100:00:00, 00:100:00, 70:20:10, 00:50:50, 50:00:50, 40:40:20 40:20:40, 20:70:10 y 20:40:40 % de ballico perenne, ovillo y trébol blanco (BP:O:TB), mismas que se distribuyeron aleatoriamente en 27 unidades experimentales bajo un diseño de bloques completos al azar con tres repeticiones. Se determinó el rendimiento de forraje, com...

  20. Evaluación microbiológica y sensorial de fermentados de pozol blanco, con cacao (Theobroma cacao) y coco (Cocos nucifera)

    OpenAIRE

    Román Jiménez Vera; Nicolás González Cortés; Arturo Magaña Contreras; Alma Corona Cruz

    2010-01-01

    El pozol es una bebida de maíz que se consume en el sureste de México y en algunos países de Centroamérica. Se puede consumir recién elaborado o fermentado. Tradicionalmente se consume solo (pozol blanco), aunque también es común agregarle cacao o coco. En este trabajo se evaluaron cambios microbiológicos durante la fermentación natural a temperatura ambiental, de tres tipos de pozol: blanco, con cacao y coco. La concentración de bacterias coliformes disminuyó a partir del tercer día de ferme...

  1. Analysis of constituents of metal elements and amino acids in new cultivar Citrus reticulata Blanco cv. Ougan fruit from China.

    Science.gov (United States)

    Chen, Xiangtao; Yuan, Ke; Si, Jinping

    2009-01-01

    The aim of this research is to establish a new kind of simple and rapid method to determine amino acids and metal elements in Citrus reticulata Blanco cv. Ougan fruit. The high-performance liquid chromatography pre-column derivation method was used to test the contents of amino acids found in the cv. fruit. The results showed that there were 17 kinds of amino acids in the fruits of two different mature periods. They could be separated easily within 30 min. The correlation coefficients between the peak area of amino acid and the content of the amino acid were above 0.99. The sample-added recovery rate of amino acids was between 96.0% and 102.4%. Meanwhile, the microwave-aided dissolving procedure was adopted for dissolving of the cv. fruit to determine the 15 metal elements in the cv. fruit under the best conditions of flame atomic absorption spectrophotometry. The results showed that there were high contents of trace elements and amino acids in the fruit of two different ripening periods. The correlation coefficients between the peak areas of amino acids and the concentration of the elements are satisfactory. These results may provide us with the scientific evidence for further studies and the exploitation of C. reticulata Blanco cv. Ougan.

  2. Radionuclide Migration at the Rio Blanco Site, A Nuclear-stimulated Low-permeability Natural Gas Reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Clay A. Cooper; Ming Ye; Jenny Chapman; Craig Shirley

    2005-10-01

    The U.S. Department of Energy and its predecessor agencies conducted a program in the 1960s and 1970s that evaluated technology for the nuclear stimulation of low-permeability gas reservoirs. The third and final project in the program, Project Rio Blanco, was conducted in Rio Blanco County, in northwestern Colorado. In this experiment, three 33-kiloton nuclear explosives were simultaneously detonated in a single emplacement well in the Mesaverde Group and Fort Union Formation, at depths of 1,780, 1,899, and 2,039 m below land surface on May 17, 1973. The objective of this work is to estimate lateral distances that tritium released from the detonations may have traveled in the subsurface and evaluate the possible effect of postulated natural-gas development on radionuclide migration. Other radionuclides were considered in the analysis, but the majority occur in relatively immobile forms (such as nuclear melt glass). Of the radionuclides present in the gas phase, tritium dominates in terms of quantity of radioactivity in the long term and contribution to possible whole body exposure. One simulation is performed for {sup 85}Kr, the second most abundant gaseous radionuclide produced after tritium.

  3. Rethinking the Role of Development Banks in Climate Finance: Panama’s Barro Blanco CDM Project and Human Rights

    Directory of Open Access Journals (Sweden)

    Beatriz Felipe Pérez

    2016-06-01

    Full Text Available Development banks are key actors in climate finance. During the last decades, they have increased the funding of climate change related projects, especially those under the Clean Development Mechanism (CDM. Defined in Article 12 of the Kyoto Protocol, the CDM aims at contributing to climate change mitigation while assisting in achieving sustainable development. However, many CDM projects have caused environmental damage and human rights abuses that especially affect the most vulnerable people. Located in Panama, the Barro Blanco hydro-power dam exemplifies the complex interrelationship of climate financing, development policies, the political and economic national context and human rights. Through the analysis of the role of development banks in climate finance, especially in the context of CDM projects, this paper aims (1 to clarify the role of development banks in climate finance, (2 to shed light on the vulnerable situation of the people affected by these projects, (3 to highlight the gaps in both the CDM rules and the development banks’ safeguard policies concerning the protection of human rights and the prevention of environmental abuses, and (4 to give a current example of this complex situation through the Barro Blanco case study. This paper argues that the manifold and often competing national and international legal and political layers of climate change mitigation projects repeatedly leave project affected people vulnerable to human rights violations without adequate safeguards and mechanisms to effectively articulate their interests, protect their rights and promote access to justice.

  4. Pelacakan Virus Bercak Putih pada Udang Vaname (Litopenaeus vannamei di Lombok dengan Real-Time Polymerase Chain Reaction (DETECTION OF WHITE SPOT SYNDROME VIRUS IN LITOPENAEUS VANNAMEI IN LOMBOK ISLAND USING REAL-TIME POLYMERASE CHAIN REACTION

    Directory of Open Access Journals (Sweden)

    Lulu Arafani

    2016-03-01

    Full Text Available White spot syndrome virus (WSSV is one of the most threatening diseases in shrimp and othercrustaceans affecting global shrimp farming. Since firstly detected in Taiwan in 1992, the disease hasspread globally and followed with considerable socio-economic consequences. This research was performedto detect the WSSV infection in shrimp farming in Lombok Island’s (West Nusa Tenggara using real-timepolymerase chain reaction. Samples of vaname (Litopenaeus vannamei were collected from several shrimpfarming in Lombok. Results indicated that the spread of WSSV has reached shrimp farms in Lombok,especially in Lendang Jae, West Lombok. Therefore, a biosurveillance program is strongly recommendedto government to avoid and halt the spread of the disease in East Indonesia region .

  5. The known two types of transglutaminases regulate immune and stress responses in white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Chang, Chin-Chyuan; Chang, Hao-Che; Liu, Kuan-Fu; Cheng, Winton

    2016-06-01

    Transglutaminases (TGs) play critical roles in blood coagulation, immune responses, and other biochemical functions, which undergo post-translational remodeling such as acetylation, phosphorylation and fatty acylation. Two types of TG have been identified in white shrimp, Litopenaeus vannamei, and further investigation on their potential function was conducted by gene silencing in the present study. Total haemocyte count (THC), differential haemocyte count (DHC), phenoloxidase activity, respiratory bursts (release of superoxide anion), superoxide dismutase activity, transglutaminase (TG) activity, haemolymph clotting time, and phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were measured when shrimps were individually injected with diethyl pyrocarbonate-water (DEPC-H2O) or TG dsRNAs. In addition, haemolymph glucose and lactate, and haemocytes crustin, lysozyme, crustacean hyperglycemic hormone (CHH), transglutaminaseI (TGI), transglutaminaseII (TGII) and clotting protein (CP) mRNA expression were determined in the dsRNA injected shrimp under hypothermal stress. Results showed that TG activity, phagocytic activity and clearance efficiency were significantly decreased, but THC, hyaline cells (HCs) and haemolymph clotting time were significantly increased in the shrimp which received LvTGI dsRNA and LvTGI + LvTGII dsRNA after 3 days. However, respiratory burst per haemocyte was significantly decreased in only LvTGI + LvTGII silenced shrimp. In hypothermal stress studies, elevation of haemolymph glucose and lactate was observed in all treated groups, and were advanced in LvTGI and LvTGI + LvTGII silenced shrimp following exposure to 22 °C. LvCHH mRNA expression was significantly up-regulated, but crustin and lysozyme mRNA expressions were significantly down-regulated in LvTGI and LvTGI + LvTGII silenced shrimp; moreover, LvTGII was significantly increased, but LvTGI was significantly decreased in LvTGI silenced shrimp

  6. PENGARUH STARVASI RANSUM PAKAN TERHADAP PERTUMBUHAN, SINTASAN, DAN PRODUKSI UDANG VANAMEI (Litopenaeus vannamei DALAM WADAH TERKONTROL

    Directory of Open Access Journals (Sweden)

    Suwardi Tahe

    2008-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh pengurangan ransum pakan secara periodik terhadap pertumbuhan, sintasan, produksi, rasio konversi pakan, dan efisiensi pakan pada pemeliharaan udang vanamei dalam wadah terkontrol. Penelitian dilakukan menggunakan 12 akuarium berukuran 50 cm x 75 cm x 60 cm dan dilengkapi dengan sistem aerasi. Hewan uji adalah pascalarva udang vanamei dengan bobot awal rata-rata 0,18 ± 0,02 g yang ditebar dengan kepadatan 50 ekor/akuarium. Rancangan penelitian adalah rancangan acak lengkap dengan empat perlakuan yang masing-masing perlakuan terdiri atas tiga ulangan. Perlakuan yang diujikan adalah starvasi (pemuasaan melalui pengurangan ransum pakan secara periodik yaitu A pengurangan ransum pakan 75%, B pengurangan ransum pakan 50%, C pengurangan ransum pakan 25%, dan D kontrol (tanpa pengurangan ransum pakan. Hasil penelitian menunjukkan bahwa pengurangan ransum pakan secara periodik tidak berpengaruh nyata (P > 0,05 terhadap pertambahan bobot biomassa, laju pertumbuhan harian, sintasan, produksi, dan rasio konversi pakan serta mampu meningkatkan efisiensi pakan sekitar 16,04%—21,97%. Penghematan penggunaan pakan untuk udang vanamei dapat dilakukan dengan pengurangan ransum pakan hingga 75% bobot biomassa/minggu. The aim of this study was to know the effect of starvation period by a gradual decrease in feed amounts on the growth, survival rate, productivity, food conversion ration (FCR, and food efficiency rate of white leg shrimp (Litopenaeus vannamei in controlled containers. Twelve of 50 cm x 75 cm x 60 cm aquaria with aeration systems were used in this experiment. In each aquarium, we stocked 50 post larvae (PL with average weight of 0.18 ± 0.02 g. Four treatments, comprising, A a 75% decrease in feed, B a 50% decrease in feed, C a 25% decrease in feed, and D control (without feed reduction with three replications were employed in the experiment following complete randomized design (DSG. The results

  7. PENGARUH PENAMBAHAN KALIUM PADA MASA ADAPTASI PENURUNAN SALINITAS TERHADAP PERFORMA PASCALARVA UDANG VANAMEI (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Ferdinand Hukama Taqwa

    2008-12-01

    Full Text Available Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan kalium selama masa adaptasi penurunan salinitas terhadap performa pascalarva udang vanamei. Penelitian ini dilaksanakan untuk mendapatkan kadar kalium optimal yang dapat menurunkan tingkat stres dan meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas. Hewan uji yang digunakan adalah PL20 udang vanamei. Rancangan percobaan menggunakan rancangan acak lengkap dengan perlakuan yang diterapkan adalah penambahan kalium ke air tawar pengencer masing-masing sebanyak 0 mg/L (A, 25 mg/L (B, 50 mg/L (C, dan 75 mg/L (D. Penurunan salinitas dilakukan secara gradual selama 4 hari dari salinitas 25 ppt hingga mencapai 2 ppt. Hasil percobaan menunjukkan bahwa penambahan kalium sebanyak 25 mg/L hingga kadar kalium media menjadi 51 mg/L dapat mengurangi pembelanjaan energi untuk osmoregulasi, tingkat stres, dan laju metabolisme standar sehingga meningkatkan sintasan pascalarva udang vanamei setelah melalui masa adaptasi penurunan salinitas selama 96 jam (4 hari. The objective of this research was to study the effect of potassium addition during salinity acclimatization from 25 ppt down to 2 ppt on the performance of Litopenaeus vannamei postlarvae. This experiment was done to determine optimal dosage of potassium which can reduce stress level and increase survival rate of L. vannamei postlarvae after salinity acclimatization. Specimen test used was PL20 of white shrimp (0.001 g. Experimental design used completely randomized design with four treatments and three replications of different potassium addition levels to freshwater: 0 mg/L (A, 25 mg/L (B, 50 mg/L (C, and 75 mg/L (D. Dilution of salinity was done gradually using freshwater during 4 days from 25 ppt down to 2 ppt. The result of this experiment indicated that the addition of 25 mg/L potassium (potassium level in media was 51 mg/L reduced the energy cost for osmoregulation, level of stress and

  8. PRODUCTOS NATURALES COMO ESTIMULADORES DEL SISTEMA INMUNOLÓGICO DE Litopenaeus vannamei, INFECTADO CON Vibrio parahaemolyticus

    Directory of Open Access Journals (Sweden)

    Nelson Pe\\u00F1a-Navarro

    2013-01-01

    Full Text Available El objetivo de este trabajo fue determinar la respuesta inmunológica de Litopenaeus vannamei con manano-oligosacáridos (T1, ajo (T2 y un compuesto de extractos de plantas (T3 después de ser infectados con Vibrio parahaemolyticus. Se realizaron dos bioensayos con una duración de seis (1x106 UFC y catorce (3x106 UFC días en el Centro de Investigación en Ciencias del Mar y Limnología de la Universidad de Costa Rica durante el 2011. Para esta investigación se extrajeron 120 camarones para cada periodo experimental de fincas ubicadas en la Península de Nicoya. Al finalizar la dosificación de los productos se evaluó la ganancia de peso y la conversión alimenticia. Luego de la infección con V. parahaemolyticus se realizaron hemogramas, coagulación, bacteriología de hemolinfa y mortalidad acumulada. Los parámetros inmunológicos no mostraron diferencias estadísticas (P>0,05 entre tratamientos en ningún periodo, sin embargo a los seis días T1 mostró los mejores resultados con 41,07x105 hemocitos/ml; una coagulación de 34,40 s y 4,44x103 UFC/ ml. En el periodo de catorce días T2 obtuvo los mejores valores (55,76x105 hemocitos/ml; una coagulación de 34,20 s y15,4x103 UFC/ml. La mortalidad acumulada se presentó a las diez horas de inoculación, hubo menor cantidad de muertes en T1 y T3 (76,2% a los seis días, mientras que a los catorce días fue para T1 (93,2%. La ganancia de peso y la conversión alimenticia resultaron con diferencias estadísticas (P<0,05 solo para el bioensayo que se extendió por seis días, donde T2 presentó una biomasa de 54,3 g, un incremento en la ganancia de peso de 19,3% y una conversión alimenticia de 1,4.

  9. Compositions and yield of lipids extracted from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) as affected by prior autolysis.

    Science.gov (United States)

    Senphan, Theeraphol; Benjakul, Soottawat

    2012-09-15

    Compositions and yield of lipids extracted from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) subjected to autolysis at 60°C for different times (0, 30, 60, 90 120 and 150 min) were investigated. Extraction yield increased from 7.4% to 8.8% as autolysis time increased from 0 to 150 min. Coincidental increase in total carotenoid content was obtained with increasing autolysis time (pautolysis time increased (pautolysis (p>0.05), but subsequently increased up to 150 min. (pautolysis were observed. Lipids extracted contained docosahexaenoic acid (DHA; C22:6(n-3)) as the most abundant fatty acid, followed by eicosapentaenoic acid (EPA; C20:5(n-3)). Therefore, prior autolysis at 60°C for 60 min increased the extraction yield without negative effect on lipid quality. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Effect of inactive yeast cell wall on growth performance, survival rate and immune parameters in Pacific White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Rutchanee Chotikachinda

    2008-10-01

    Full Text Available Effects of dietary inactive yeast cell wall on growth performance, survival rate, and immune parameters in pacific white shrimp (Litopenaeus vannamei was investigated. Three dosages of inactive yeast cell wall (0, 1, and 2 g kg-1 were tested in three replicate groups of juvenile shrimps with an average initial weight of 7.15±0.05 g for four weeks. There was no significant difference in final weight, survival rate, specific growth rate, feed conversion ratio, feed intake, protein efficiency ratio, and apparent net protein utilization of each treatments. However, different levels of inactive yeast cell wall showed an effect on certain immune parameters (p<0.05. Total hemocyte counts, granular hemocyte count, and bacterial clearance were better in shrimp fed diets supplemented with 1 and 2 g kg-1 inactive yeast cell wall as compared with thecontrol group.

  11. Dietary Immunogen®modulated digestive enzyme activity and immune gene expression in Litopenaeus vannamei post larvae.

    Science.gov (United States)

    Miandare, Hamed Kolangi; Mirghaed, Ali Taheri; Hosseini, Marjan; Mazloumi, Nastaran; Zargar, Ashkan; Nazari, Sajad

    2017-11-01

    Pacific white shrimp Litopenaeus vannamei (Boone, 1931) is an important economical shrimp species worldwide, especially in the Middle East region, and farming activities of this species have been largely affected by diseases, mostly viral and bacterial diseases. Scientists have started to use prebiotics for bolstering the immune status of the animal. This study aimed to investigate the influence of Immunogen ® on growth, digestive enzyme activity and immune related gene expression of Litopenaeus vannamei post-larvae. All post-larvae were acclimated to the laboratory condition for 14 days. Upon acclimation, shrimps were fed on different levels of Immunogen ® (0, 0.5, 1 and 1.5 g kg -1 ) for 60 days. No significant differences were detected in weight gain, specific growth rate (SGR) and food conversion ratio (FCR) in shrimp post-larvae in which fed with different levels of Immunogen ® and control diet. The results showed that digestive enzymes activity including protease and lipase increased with different amounts of Immunogen ® in the shrimp diet. Protease activity increased with 1.5 g kg -1 Immunogen ® after 60 days and lipase activity increased with 1 and 1.5 g kg -1 Immunogen ® after 30 and 60 days of the trial respectively (P  0.05). The expression of immune related genes including, prophenoloxidase, crustin and g-type lysozyme increased with diet 1.5 g kg -1 Immunogen ® (P digestive enzymes activity and expression of immune related genes could modulate the Immunogen ® in the innate immune system in L. vannamei in this study. Copyright © 2017. Published by Elsevier Ltd.

  12. A ten-month diseases survey on wild Litopenaeus setiferus (Decapoda: Penaeidae from Southern Gulf of Mexico

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    Rodolfo Enrique del Río-Rodríguez

    2013-09-01

    Full Text Available The development of shrimp aquaculture in Mexican coasts of the Gulf of Mexico began to be explored using the Pacific white shrimp Litopenaeus vannamei in the mid 90´s. Many concerns over the risk of disease transmission to the economically important native penaeids, have been the main deterrent for the aquaculture of L. vannamei in the region. Concurrently, more than 10 years of research experience on the aquaculture suitability of the native Litopenaeus setiferus from the Terminos Lagoon, in the Yucatán Peninsula, have been accumulated. The aim of this study was then to determine the seasonal variations of the naturally acquired diseases and the possible detection of exotic pathogens. For this, random subsamples (n~60 of juveniles L. setiferus were collected from monthly captures. In order to detect the widest range of pathogens, including infectious hypodermal and hematopoietic necrosis (IHHNv and white spot syndrome (WSSv viruses, both histopathological and molecular methods were employed. Monthly prevalence (% was calculated for every finding. We were able to detect a total of 16 distinct histological anomalies, most of which the presump- tive aetiological agent was readily identified. PCR results for viruses were negative. For some pathogens and symbionts, the prevalence was significantly different between the adult and juvenile populations. Prevalence of diseases tended to be higher in juvenile shrimp than in adults. The results of this study indicated that L. setiferus carry a wide variety of pathogens and symbionts that seem to be endemic to penaeids of the Gulf of Mexico, and those juveniles were more conspicuous to acquire pathogens and symbionts than adults.

  13. Physical Analysis of the Complex Rye (Secale cereale L.) Alt4 Aluminium (Aluminum) Tolerance Locus Using a Whole-Genome BAC Library of Rye cv. Blanco

    Science.gov (United States)

    Rye is a diploid crop species with many outstanding qualities, and is also important as a source of new traits for wheat and triticale improvement. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies. The library provides a 6 × genome ...

  14. PROPAGACIÓN DE Tabebuia Donnell-Smithii Rose (GUAYACÁN BLANCO UTILIZANDO HORMONAS DE ENRAIZAMIENTO

    Directory of Open Access Journals (Sweden)

    Mercedes Susana Carranza Patiño

    2013-02-01

    Full Text Available Resumen La amplia distribución y abundancia natural de Tabebuia donnell-smithii (guayacán blanco se ha visto reducida por la tala excesiva de los árboles, lo que ha ocasionado la desaparición de genotipos valiosos así como disminución de las poblaciones naturales, existiendo además carencia de alternativas de producción de plantas a gran escala. Se hace por tanto necesario orientar la investigación a establecer una técnica para la propagación vegetativa de guayacán blanco utilizando fitorreguladores. La metodología se basó en el uso de las hormonas de enraizamiento ácido naftalenacético (ANA y ácido indolbutírico (AIB, en concentraciones de 0, 1,500 y 2,000 mg kg-1 en sustratos turba y arena. Las yemas apicales fueron colocadas en una cámara húmeda en condiciones de invernadero. Se aplicó un Diseño Completamente al Azar (DCA en un arreglo factorial 2 sustratos x 3 dosis de hormona ANA x 3 dosis de hormona AIB, con cuatro repeticiones y cuatro unidades de observación. A los 45 días se evaluó el porcentaje de sobrevivencia y el enraizamiento, el número de raíces, la longitud de la raíz mayor, el número de brotes, la longitud de brotes, y el vigor. Los resultados no mostraron diferencias significativas entre los distintos tratamientos para todas las variables, sin embargo para el efecto simple e interacciones se observó diferencias para las variables evaluadas, siendo el mejor sustrato turba y las concentraciones óptimas de auxinas de 0 a 1,500 mg Kg-1. Se concluye que el guayacán blanco es una especie de fácil enraizamiento, ya que con y sin la aplicación de hormonas enraizadoras en el sustrato turba y arena se logró la obtención de clones, disminuyendo el tiempo de obtención de nuevas plantas.

  15. Geomorphic change along a gravel bed river affected by volcanic eruption: Rio Blanco - Volcan Chaiten (South Chile)

    Science.gov (United States)

    Picco, Lorenzo; Ravazzolo, Diego; Ulloa, Hector; Iroumé, Andres; Aristide Lenzi, Mario

    2014-05-01

    Gravel bed rivers are environments shaped by the balance of flow, sediment regimes, large wood (LW) and vegetation. Geomorphic changes are response to fluctuations and changes of runoff and sediment supply involving mutual interactions among these factors. Typically, many natural disasters (i.e. debris flows, floods and forest fires) can affect the river basin dynamics. Explosive volcanic eruptions present, instead, the potential of exerting severe impacts as, for example, filling river valleys or changing river network patterns thanks to massive deposition of tephra and volcanic sediment all over the main channel and over the basin. These consistent impacts can strongly affect both hydrology and sediment transport dynamics, all over the river system, producing huge geomorphic changes. During the last years there has been a consistent increase in the survey technologies that permit to monitor geomorphic changes and to estimate sediment budgets through repeat topographic surveys. The calculation of differences between subsequent DEMs (difference of DEMs, DoD) is a commonly applied method to analyze and quantify these dynamics. Typically the higher uncertainty values are registered in areas with higher topographic variability and lower point density. This research was conducted along a ~ 2.2 km-long sub-reach of the Blanco River (Southern Chile), a fourth-order stream that presents a mainly rainfall regime with winter peak flows. The May 2008 Chaitén volcanic eruption strongly affected the entire Rio Blanco basin. The entire valley was highly exposed to the pyroclastic and fluvial flows, which affected directly a consistent area of evergreen forests. Extreme runoff from the upper Blanco catchment aggraded the channel and deposited up to several meters of tephra, alluvium, and LW along the entire river system. Aims of this contribution are to define and quantify the short term evolution of the Blanco River after the big eruption event and a subsequent consistent

  16. Citrus Essential Oil of Nigeria Part IV: Volatile Constituents of Leaf Oils of Mandarins (Citrus Reticulata Blanco From Nigeria

    Directory of Open Access Journals (Sweden)

    Adeleke A. Kasali

    2010-07-01

    Full Text Available The chemical composition of hydrodistilled oils obtained from the leaves of six Citrus reticulata Blanco (mandarin cultivars grown in Nigeria were examined by GC and GC/MS, the result of their chemical composition were further submitted to cluster analysis. Fifty seven constituents were characterized accounting for 88.2 - 96.7% of the total oils. Sabinene, g -terpinene, P-cymene, d -3-carene and (E- b -ocimene were observed in great variability in all the oils. Other constituents include linalool, myrcene, terpinen-4-ol and cis-sabinenehydrate. In addition, limonene, terpinolene, b -pinene, and a -pinene were also detected in appreciable concentrations. b -sinensal and a -sinensal were isolated by preparative GC and characterized by one- and two-dimensional NMR techniques.

  17. Ricardo Piglia, autor de relatos policiais. Uma leitura comparada de Plata Quemada (1997 e Blanco Nocturno (2010

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    Michelly Cristina da Silva

    2017-02-01

    Full Text Available O presente artigo visa comparar dois romances de Ricardo Piglia, a saber, Plata Quemada (1997 e Blanco Nocturno (2010. As duas histórias representam as incursões novelescas do autor pelo mundo dos romances policiais. Em outra ocasião, em um ensaio crítico intitulado “Sobre el género policial”, Piglia apresentou duas divisões dentro do gênero, situando-as cronologicamente e destacando suas diferenças: o romance de “enigma” ou analítico, por um lado, e o hard-boiled ou policial duro, de outro. Interessa-nos aqui indicar nos dois livros do autor elementos do relato policial citados pelo mesmo, observando como os dois romances, no tocante à narrativa, se assemelham e se  distanciam.

  18. Magnetic molecularly imprinted polymer for the selective extraction of hesperetin from the dried pericarp of Citrus reticulata Blanco.

    Science.gov (United States)

    Wang, Dan-Dan; Gao, Die; Xu, Wan-Jun; Li, Fan; Yin, Man-Ni; Fu, Qi-Feng; Xia, Zhi-Ning

    2018-07-01

    In present study, novel magnetic molecularly imprinted polymers for hesperetin were successfully prepared by surface molecular imprinting method using functionalized Fe 3 O 4 particles as the magnetic cores. Hesperetin as the template, N-Isopropylacrylamide as the functional monomer, ethylene glycol dimethyl acrylate as the crosslinker, 2,2-azobisisobutyonnitrile as initiator and acetonitrile-methanol (3:1, v/v) as the porogen were applied in the preparation process. Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscope, x-ray diffraction and vibrating sample magnetometry were applied to characterize the magnetic molecularly imprinted polymers. The adsorption experiments indicated that the magnetic molecularly imprinted polymers performed high selective recognition property to hesperetin. The selectivity experiment indicated that the adsorption capacity and selectivity of polymers to hesperetin was higher than that of luteolin, baicalein and ombuin. Furthermore, the magnetic molecularly imprinted polymers were employed as adsorbents for extraction and enrichment of hesperetin from the dried pericarp of Citrus reticulata Blanco. The recoveries of hesperetin in the dried pericarp of Citrus reticulata Blanco ranged from 90.5% to 96.9%. The linear range of 0.15-110.72 µg/mL was obtained with correlation coefficient of greater than 0.9991. The limit of detection and quantification of the proposed method was 0.06 µg/mL and 0.15 µg/mL, respectively. Based on three replicate measurements, intra-day RSD was 0.71% and inter-day RSD was 2.31%. These results demonstrated that the prepared magnetic molecularly imprinted polymers were proven to be an effective material for the selective adsorption and enrichment of hesperetin from natural medicines, fruits and et al. Copyright © 2018 Elsevier B.V. All rights reserved.

  19. Geology and metallogeny of the volcanic complex of Rio Blanco Ullum. Province of San Juan. Republica Argentina

    International Nuclear Information System (INIS)

    Mendoza, N.; Weidmann, N.; Puigdomenech, H.; Weidmann, R.

    2007-01-01

    Preliminary results of a research carried out at the Complejo Rio Blanco de Ullum, San Juan. Argentina are summarized in the present paper. These studies are focused on geological and metallogenic features o f this unit. The study area is located 20 km. WNW of San Juan city with geographic coordinates of 31grades 30' South latitude and 68 grades 52' West longitude. The older rocks aotcroping in the area correspond to limestones of Ordovician San Juan Formation, the chronologic succession continues with sales and siltstones of Silurian Tambolar Formation, pelites and subgraywackes of Devonian Punta Negra Formation and finally a 1500 m thick package of piroclastics and sediments of Albarracin Formation of Tertiary age. Albarracin Formation is composed pf a Basal Member (sandstones and stilstones), a Tuffaceous Member (tuffs, tuffites and oligomictic breccia s with conglomerate interbed dings in the upper part) and a Conglomeratic Member (polimictic para conglomerates). According to piroclastics facies, relationships and spreading area of piroclastics deposits a c olapsed dome and avalanche model is proposed to be the main process for the piroclastics package outcropping in the area.Sedimentary and piroclastics rocks are intruded by five sub volcanic units as noted by Leveratto (1968) which are composed by different lithologies such as: Altered Da cite - Rhyolite, Ullum Da cite, Cerro Blanco de Zonda Andesite, Ullum Andesite and Hybrid Andesite.Detailed work on alteration assemblages and metallogenic features in the southwestern sector of the Complejo resulted in the identification of three alteration zones with characteric features of potassic, argillic and propyllitic signature. (author)

  20. Caracterización agronómica de una accesión de Swinglea glutinosa (Blanco Merr, (Rutaceae

    Directory of Open Access Journals (Sweden)

    Rafael jiménez Villasuso

    2015-06-01

    Full Text Available La familia Rutaceae abarca un gran número de géneros y especies. De ellos, Citrus, Poncirus y Fortunella son los géneros más explotados comercialmente a nivel mundial, sin embargo, hay otros menos abordados, como el género Swinglea que posee una sola especie: S. glutinosa (Blanco Merr. Con el objetivo de caracterizar desde el punto de vista agronómico esta especie por su de importancia como recurso genético y posterior utilización como patrón en la producción citrícola. Se evaluaron las características físico-químicas de los frutos. Para ello se analizaron las variables: masa, diámetro y altura del fruto, número y masa de las semillas, sólidos solubles totales, acidez, índice de madurez, contenido de vitamina C y algunos caracteres cualitativos de la morfología del árbol como: estructura del árbol, formas del fruto, de las hojas y la semilla. Los resultados obtenidos mostraron que es un árbol rustico, adaptándose bien a nuestras condiciones, además presenta un alto número de semillas por fruto. Los resultados obtenidos mostraron que es un árbol rustico, adaptándose bien a nuestras condiciones, además presenta un alto número de semillas por fruto, lo cual es de gran importancia a la hora de seleccionar un patrón para su introducción a la práctica productiva. Agronomic characterization of an accession of Swinglea glutinosa (Blanco Merr, (Rutaceae

  1. CARACTERIZACIÓN COLORIMÉTRICA DEL MANJAR BLANCO DEL VALLE CARACTERIZAÇÃO COLORIMÉTRICA DE MANJAR BRANCO DEL VALLE COLORIMETRIC CHARACTERIZATION OF MANJAR BLANCO DEL VALLE

    Directory of Open Access Journals (Sweden)

    DIEGO FABIÁN NOVOA

    2012-12-01

    Full Text Available El Manjar Blanco del Valle originario de la región del Valle del Cauca en Colombia es considerado como símbolo gastronómico y cultural; es un tipo de dulce de leche con adición de almidón. El color es uno de los atributos más importantes en los alimentos. La medición del color tiene aplicaciones en el control de calidad y el desarrollo de nuevos productos. En esta investigación se caracteriza el color instrumental (escala CIE-L*a*b* del manjar blanco del Valle elaborado por empresas representativas y tradicionales de la región, mediante la determinación de los parámetros de color (L*, a*, b* de tres lotes de cada una de las marcas comerciales. También se caracterizó físicamente las muestras, encontrando pH entre 5,73 y 6,02 y grados Brix entre 65,16 y 76,47. Los valores promedio de los parámetros de color determinados experimentalmente para el manjar blanco fueron: L* 43,60, a* 14,58 y b* 34,67. De los datos reportados en este trabajo se concluye que existe una variabilidad considerable en el color entre lotes y marcas comerciales, lo que evidencia la necesidad de establecer procedimientos de estandarización en la producción y en la materia prima, con el fin de lograr un producto con características similares.O Manjar Branco Del Valle originário da região do Vale de Cauca na Colômbia é considerado um símbolo gastronômico e cultural; é um tipo de doce de leite com adição de amido. A coloração é um dos atributos mais importantes nos alimentos. A determinação da cor tem aplicações no controle de qualidade e no desenvolvimento de novos produtos. Neste estudo se caracterizou a cor instrumental (Sistema CIE-L*a*b* do Manjar Branco Del Valle produzido por empresas representativas e tradicionais da região, mediante a determinação dos parâmetros de cor (L*, a*, b* de três lotes de cada uma das marcas comerciais. Também se caracterizou fisicamente as amostras, encontrando pH entre 5,73 e 6,02 e graus Brix entre 65

  2. QUALITY OF THE WATER IN SHRIMP FARM Litopenaeus vannamei WITH SYSTEM OF PARTIAL RECIRCULATION SYSTEM. QUALIDADE DA ÁGUA EM FAZENDA DE CAMARÃO MARINHO Litopenaeus vannamei COM SISTEMA DE RECIRCULAÇÃO PARCIAL.

    Directory of Open Access Journals (Sweden)

    Luiz Otávio Brito

    2008-12-01

    Full Text Available The present work aim to characterize the quality of the water affluent and effluent of a shrimp Litopenaeus vannamei farm , which operates with system of partial recirculation system. The samples were accomplished during the low water and high tide periods of the day. The analyzed variables were: dissolved oxygen, temperature, pH, salinity, ammonia, nitrate, nitrite and phosphate. They samples were seven points, distributed strategically in way to characterize the water from the entrance going by the inside of the to your exit farm. ANOVA and Tukey test (P<0,05 were used in statistical analysis. Was observed the pH and dissolved oxygen stayed presenting averages of 7.72 and 6.58mg.L-1, respectively. The final averages went from 1.40 mg.L-1 to ammonia, 0.76 mg.L-1 for nitrate, 0.08 mg.L-1 for nitrite and 1.63 mg.L-1 for the phosphate.

    KEY WORDS: Quality of the water, recirculation, shrimp, vannamei.
    O presente trabalho teve como objetivo caracterizar a qualidade da água afluente e efluente de uma fazenda de camarão marinho Litopenaeus vannamei, que opera com sistema de recirculação parcial de água. As coletas foram realizadas durante os períodos de baixamar e preamar, diurnas, nos dias de lua. Analisaram-se as seguintes variáveis: oxigênio dissolvido, temperatura, pH, salinidade, amônia, nitrato, nitrito e fosfato. Escolheram-se sete pontos de coleta, distribuídos estrategicamente, de modo a caracterizar a água desde a entrada, passando pelo interior da fazenda até sua saída. Para análise estatística, utilizaram-se análise de variância (ANOVA e teste de Tukey (P<0,05. Observou-se que o pH e o oxigênio dissolvido apresentaram uma média de 7,72 e 6,58mg.L-1, respectivamente. As médias finais foram de 1,40mg.L-1 para amônia, 0,76mg.L-1 para nitrato, 0,08mg.L-1 para nitrito e 1,63mg.L-1 para o fosfato.

    PALAVRAS-CHAVES: Camarão, vannamei, qualidade da água, recirculação

  3. Calidad de agua en un sistema de recirculación con biofiltros para la producción intensiva de postlarvas de camarón litopenaeus vannamei

    OpenAIRE

    Arcos Correa, Danny; Sonnenholzner, Stanislaus; Townsend, Soraya

    2002-01-01

    Calidad de agua en un sistema de recirculación con biofiltros para la producción intensiva de postlarvas de camarón Litopenaeus vannamei La industria de la Acuicultura está enfrentando serios cambios técnicos con la finalidad de desarrollar formas económicas y efectivas de mejorar su producción. La producción de postlarvas de camarón Litopenaeus vannamei se realizan con elevados recambios de agua cercanos al 300% del volumen total, para mantener la calidad de la misma.

  4. Daily cycle and body characteristics of mating Litopenaeus vannamei shrimps (Decapoda: Penaeidae in the wild off southern Sinaloa, Mexico

    Directory of Open Access Journals (Sweden)

    J.A Calderón-Pérez

    2007-03-01

    Full Text Available Mating behavior has been described for very few species of penaeoid shrimps. We describe some of the environmental conditions under which mating of Litopenaeus vannamei took place in the ocean, as inferred by the presence of attached spermatophores on the thelycum of females, combined with the presence of mature males with empty ampulla terminalis, both evidence of recent copulation. Out of a total of twelve 24 hr samplings on board the research vessel B/O "EL PUMA", one was selected to examine mating. There were four periods of observation. The highest frequency of females with attached spermatophores were found during the daytime whilst the lowest was registered during the night. Females with attached spermatophore were mostly at stages IV and V of ovarian maturity and fell within the 30 - 50 mm of C.L. range. Copulating male size ranged between 30 and 40 mm of C.L. and there was a close relation between the percentage of mating females and males. Male to female ratio varied throughout the sampling period but it was never 1:1. The female mean size was, in all cases, bigger than the male mean size. Among the environmental factors, salinity and oxygen varied very slightly throughout the sampling period; temperature variation was more pronounced and those changes were attributed to the tidal oscillation. Rev. Biol. Trop. 55 (1: 189-198. Epub 2007 March. 31.Estudiamos el apareamiento del camarón Litopenaeus vannamei . La mayoría de las hembras con espermatóforos fueron capturadas durante el día y estaban en las etapas IV y V de desarrollo ovárico (longitud 30 - 50 mm. Los machos apareados midieron entre 30 y 40 mm. La proporción sexual varió pero nunca fue 1:1. Las hembras siempre fueron más grandes que los machos. Durante el muestreo, la salinidad y el oxígeno variaron poco. La temperatura varió más y atribuimos estos cambios a las mareas.

  5. Improvement of survival and development of Pacific white shrimp Litopenaeus vannamei larvae by feeding taurine enriched rotifers

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    Dedi Jusadi

    2015-03-01

    Full Text Available ABSTRACTThe objective of the present experiment was to study the most optimum taurine enrichment concentration of rotifers in improving Pacific white shrimp larva Litopenaeus vannamei survival and development. White shrimp larvae at sixth naupliar stage were reared in 12 units of 500 L fibre glass tanks with a stocking density of 125 ind/L. Starting from zoea two stage (Z-2, the larva was provided with rotifers with different taurine enrichment concentration according to the treatments, i.e. 0 mg/L enrichment medium (A, 25 mg/L (B, 50 mg/L(C, and 100 mg/L (D. The results show that different taurine concentration in the enrichment media increased taurine level in rotifers. Furthermore, the administration of taurine enriched rotifers up to 50 mg/L significantly improved larval survival and may accelerate larval development. The experimental results also concluded that a concentration of 50 mg/L is the most optimum taurine enrichment concentration of rotifers for the improvement of white shrimp larval survival and developmental stage.Keywords: taurine, rotifer, white shrimp, enrichmentABSTRAKPenelitian ini bertujuan untuk mengkaji konsentrasi optimum taurin melalui pengayaan pada rotifera terhadap tingkat kelangsungan hidup dan perkembangan stadia larva udang vaname Litopenaeus vannamei. Larva udang vaname stadia naupli-6 dipelihara dalam 12 tangki fiberglass volume 500 L dengan kepadatan 125 ind/L. Dimulai sejak stadia zoea 2 (Z-2 larva diberi rotifera yang diperkaya dengan taurin dengan konsentrasi yang berbeda sesuai dengan perlakuan, yaitu 0 mg/L media pengkaya (A, 25 mg/L (B, 50mg/L (C, dan 100mg/L (D. Hasil penelitian menunjukkan pengayaan taurin pada konsentrasi yang berbeda menyebabkan peningkatan kandungan taurin rotifera. Sementara pemberian rotifera yang diperkaya taurin hingga 50 mg/L meningkatkan kelangsungan hidup dan mempercepat perkembangan stadia larva udang. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian

  6. Effect of copper on the growth of shrimps Litopenaeus vannamei: water parameters and copper budget in a recirculating system

    Science.gov (United States)

    Cheng, Bo; Liu, Ying; Yang, Hongsheng; Song, Yi; Li, Xian

    2014-09-01

    Shrimps ( Litopenaeus vannamei) were intensively cultured in a recirculating aquaculture system for 98 days to investigate effects of 0.3 mg/L Cu on its performance, Cu budget, and Cu distribution. Shrimps in Cu-treated systems had greater mean final weight (11.10 vs 10.50 g), body length (107.23 vs 106.42 mm), survival rate (67.80% vs 66.40%), and yield (6.42 vs 5.99 kg/m3), and lower feed conversion ratio (1.20 vs 1.29) than those in control systems but the differences were not significant. Vibrio numbers remained stable (104-106 colony forming units/mL) in the rearing tanks of both control and treated systems. Total ammonium-N, nitrite-N, nitrate-N, pH, chemical oxygen demand, 5-day carbonaceous biochemical oxygen demand, and total suspended solids were similar in controls and treatments. Dissolved Cu concentration in the treated systems decreased from 0.284 to 0.089 mg/L while in the control systems it increased from 0.006 2 to 0.018 mg/L. The main sources of Cu in the treated systems were the artificially added component (75.7% of total input), shrimp feed (21.0%), water (2.06%), and shrimp biomass (1.22%). The major outputs of Cu occurred via the mechanical filter (41.7%), water renewal (15.6%), and draining of the sediment trap (15.1%). The foam fractionator removed only 0.69% of total Cu input. Harvested shrimp biomass accounted for 11.68% of Cu input. The Cu concentration of shrimps in the Cu-treated systems (30.70 mg/kg wet weight) was significantly higher than that in control systems (22.02 mg/kg). Both were below the maximum permissible concentration (50 mg/kg) for Cu in seafood for human consumption in China. Therefore, recirculating systems can be used for commercial on-growing of Litopenaeus vannamei without loss of shrimp quality, even in water polluted by 0.30 mg/L Cu. The mechanical filter is the main route for Cu removal.

  7. Embriões liofilizados e flocos de Artemia no cultivo pós-larval de Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424 Use of freeze-dried embryos and Artemia flakes in the post-larval culture of Litopenaeus vannamei (Boone, 1931 - DOI: 10.4025/actascibiolsci.v30i1.1424

    Directory of Open Access Journals (Sweden)

    Pedro Hercílio de Oliveira Cavalcante

    2008-03-01

    Full Text Available O objetivo do estudo foi avaliar o crescimento e sobrevivência de pós-larvas de Litopenaeus vannamei que receberam embriões liofilizados (EMB e flocos (FLO de Artemia como suplementos alimentares a uma dieta microparticulada (DMP. Nos tratamentos T1, T2 e T3, os camarões receberam DMP e após 2h, acréscimos de FLO (T1; de EMB (T2; e da mesma DMP, anteriormente, ofertada (T3. No T4, as pós-larvas foram alimentadas com DMP, mas sem suplementações posteriores. Não houve diferença significativa (p > 0,05 na sobrevivência dos camarões após o período experimental de 21 dias. Por outro lado, o ganho de peso absoluto (mg peso seco das pós-larvas, cuja dieta foi suplementada com BEM, foi significativamente superior (p Artemia e recomendam sua inclusão na dieta pós-larval de Litopenaeus vannameiThe objective of the study was to evaluate the growth and survival of Litopenaeus vannamei post-larvae fed with freeze-dried embryos and Artemia flakes as feeding supplements to a microparticulate diet (DMP. In treatments T1, T2 and T3, the shrimp were fed with DMP and after 2 hours, received supplements of FLO (T1; EMB (T2; and the same DMP previously offered (T3. In T4, post-larvae were fed with DMP but without additional supplementation. There was no significant difference (p > 0.05 in shrimp survival after the experimental period of 21 days. On the other hand, the absolute weight gain (mg of dry weight of post-larvae whose diet was supplemented with EMB (T2 was significantly higher (p Artemia embryos and recommend their inclusion in the post-larval diet of Litopenaeus vannamei

  8. Caracterización agronómica una accesión de Swinglea glutinosa (Blanco Merr, (Rutaceae

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    Rafael Jiménez Villasuso

    2015-05-01

    Full Text Available RESUMEN La familia Rutaceae abarca un gran número de géneros y especies. De ellos, Citrus, Poncirus y Fortunella son los géneros más explotados comercialmente a nivel mundial, sin embargo, hay otros menos abordados, como el género Swinglea que posee una sola especie: S. glutinosa (Blanco Merr. Con el objetivo de caracterizar desde el punto de vista agronómico esta especie por su de importancia como recurso genético y posterior utilización como patrón en la producción citrícola. Se evaluaron las características físico-químicas de los frutos. Para ello se analizaron las variables: masa, diámetro y altura del fruto, número y masa de las semillas, sólidos solubles totales, acidez, índice de madurez, contenido de vitamina C y algunos caracteres cualitativos de la morfología del árbol como: estructura del árbol, formas del fruto, de las hojas y la semilla. Los resultados obtenidos mostraron que es un árbol rustico, adaptándose bien a nuestras condiciones, además presenta un alto número de semillas por fruto. Los resultados obtenidos mostraron que es un árbol rustico, adaptándose bien a nuestras condiciones, además presenta un alto número de semillas por fruto, lo cual es de gran importancia a la hora de seleccionar un patrón para su introducción a la práctica productiva. Agronomic characterization of an accession of Swinglea glutinosa (Blanco Merr, (Rutaceae ABSTRACT The Rutaceae family includes a large number of genera and species. Of these, Citrus, Poncirus and Fortunella are the genres most worldwide exploited commercially, but there are other less discussed, such as gender Swinglea having a single species: S. glutinosa (Blanco Merr. In order to characterize from an agricultural point of view this kind of importance as their genetic resource and subsequent use as a standard in citrus production. The physicochemical characteristics of the fruits were evaluated. mass, diameter and height of fruit number and seed mass

  9. La cerámica Blanco sobre Rojo en el valle de Chancay y sus relaciones con el estilo Lima

    Directory of Open Access Journals (Sweden)

    2003-01-01

    , realizadas en 1997. Este es uno de los pocos sitios conocidos con arquitectura monumental pública asignada a las fases iniciales del Período Intermedio Temprano y relacionada con la tradición cerámica Blanco sobre Rojo. Nuestro interés principal fue refinar la cronología del Blanco sobre Rojo en el valle bajo de Chancay mediante información estratigráfica y compararla con otros esquemas cronológicos para la costa central. Como resultado, definimos una secuencia arquitectónica de seis fases que implicaban cambios funcionales. Las tres primeras fases son de uso doméstico, con alto contenido de restos culturales. Las fases siguientes se caracterizan por la construcción de grandes muros para plataformas hechos de adobe plano-convexo. Respecto a la cerámica, tenemos una secuencia de cuatro fases netamente Blanco sobre Rojo, que guardan íntima relación con el desarrollo arquitectónico. Esta cronología puede ser correlacionada con la de Miramar, propuesta por Patterson (1961-1966. Finalmente, otro aspecto interesante era el momento de transición entre Blanco sobre Rojo y Lima. Considerando que las evidencias de ocupación Lima en Baños de Boza son escasas, revisamos la información estratigráfica de Cerro Trinidad y Playa Grande. De esta manera, observando las claras diferencias entre el Blanco sobre Rojo y Lima, suponemos que, al menos en el valle de Chancay, Lima no deriva estilísticamente del Blanco sobre Rojo sino que es un elemento foráneo. Además, ambos estilos coexisten durante un cierto período. THE WHITE-ON-RED POTTERY SEQUENCE IN THE CHANCAY VALLEY AND ITS RELATIONSHIP WITH THE LIMA STYLE. In the present article, we present the results of the archaeological investigations at Baños de Boza, Chancay Valley, made in 1997. This one of the few known sites with public monumental architecture ascribed to the initial phases of Early Intermediate Period that are characterized by the White-on-Red pottery tradition. Our main interest was to investigate the

  10. La evaluación de los recursos hídricos en el Libro Blanco del Agua en España

    OpenAIRE

    Estrela, Teodoro; Cabezas Calvo-Rubio, Francisco; Estrada Lorenzo, Federico

    1999-01-01

    En el presente artículo se describe brevemente el modelo hidrológico distribuido utilizado en el Libro Blanco del Agua en España para la evaluación de los recursos hídricos en régimen natural. Se tiene la intención de presentar en sucesivos números de esta Revista otros instrumentos o procedimientos específicamente desarrollados para el Libro Blanco que se estima pueden presentar interés técnico o científico. El objetivo de este artículo, y de los posibles artículos posteriores, es describir ...

  11. "Tres ataúdes blancos" de Antonio Ungar: juegos de falsedad y discursos de poder en la América Latina contemporánea

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    Felipe Cammaert

    2012-10-01

    Full Text Available Tres Ataúdes Blancos, novela del escritor colombiano Antonio Ungar, propone una lecturanovedosa de la realidad latinoamericana adoptando la forma de una sátira política. Esta novela escenificalos juegos de poder en Miranda, país imaginario que es una suma de los abusos del ejercicio político en elcontinente a lo largo de su historia. Para ello, elabora una profunda reflexión en torno a la falsedad y laalteridad. Tres ataúdes blancos plantea una relectura del canon latinoamericano donde la representación dela realidad política ocupa un lugar preponderante en el universo ficcional.

  12. Juan G[onzález-Blanco]. de Luaces: el traductor desconocido de la posguerra española

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    Ortega Sáez, Marta

    2009-12-01

    Full Text Available Not much has been written about the most prolific translator of post-war Spain: Juan G[onzález-Blanco]. de Luaces. During the forties he published more than a hundred translations into Spanish. Biographer, novelist and poet, Luaces was one more example of an intellectual whose career was cut short after the victory of the “nationalists” and the establishment of the Franco dictatorship. The severe censorship imposed at all levels forced him to leave aside his job as a writer to devote practically his entire life to translating in order to maintain his family. The main purpose of the following article is to offer a biographical account of a forgotten Spaniard in an attempt to contribute, albeit modestly, to recovering the country’s historical memory.Poco se ha escrito hasta el momento sobre quien podría ser denominado el traductor más prolífico de la posguerra española: Juan G[onzález-Blanco]. de Luaces. En la década de los cuarenta publicó más de cien traducciones al español. Biógrafo, novelista y poeta, Luaces fue uno de tantos intelectuales españoles cuya trayectoria profesional se vio truncada por el estallido de la Guerra Civil española y la victoria del bando “nacional”. La imposición de la dictadura franquista y la subsiguiente censura le obligó a dejar de lado su labor de escritor para dedicarse en cuerpo y alma al oficio de la traducción y poder, así, mantener a su familia. El objeto del siguiente artículo es relatar la vida de este personaje abandonado en el olvido con el afán de contribuir a completar, de forma modesta, la memoria histórica de un período de nuestra historia en el que aún quedan muchas ausencias por suplir.

  13. An improved determination of the lithium depletion boundary age of Blanco 1 and a first look on the effects of magnetic activity

    Energy Technology Data Exchange (ETDEWEB)

    Juarez, Aaron J.; Stassun, Keivan G. [Department of Physics, Fisk University, Nashville, TN 37208 (United States); Cargile, Phillip A. [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 37235 (United States); James, David J., E-mail: a.juarez@vanderbilt.edu [Cerro Tololo Inter-American Observatory, Casilla 603, La Serena (Chile)

    2014-11-10

    The lithium depletion boundary (LDB) is a robust method for accurately determining the ages of young clusters, but most pre-main-sequence models used to derive LDB ages do not include the effects of magnetic activity on stellar properties. In light of this, we present results from our spectroscopic study of the very-low-mass members of the southern open cluster Blanco 1 using the Gemini-North Telescope, program IDs: GN-2009B-Q-53 and GN-2010B-Q-96. We obtained Gemini Multi-Object Spectrograph spectra at intermediate resolution for cluster candidate members with I ≈ 13-20 mag. From our sample of 43 spectra, we find 14 probable cluster members by considering proximity to the cluster sequence in an I/I – K {sub s} color-magnitude diagram, agreement with the cluster's systemic radial velocity, and magnetic activity as a youth indicator. We systematically analyze the Hα and Li features and update the LDB age of Blanco 1 to be 126{sub −14}{sup +13} Myr. Our new LDB age for Blanco 1 shows remarkable coevality with the benchmark Pleiades open cluster. Using available empirical activity corrections, we investigate the effects of magnetic activity on the LDB age of Blanco 1. Accounting for activity, we infer a corrected LDB age of 114{sub −10}{sup +9} Myr. This work demonstrates the importance of accounting for magnetic activity on LDB inferred stellar ages, suggesting the need to reinvestigate previous LDB age determinations.

  14. Geologic and hydrostratigraphic map of the Anhalt, Fischer, and Spring Branch 7.5-minute quadrangles, Blanco, Comal, and Kendall Counties, Texas

    Science.gov (United States)

    Clark, Allan K.; Robert R. Morris,

    2015-01-01

    This report describes the geology and hydrostratigraphy of the Edwards and Trinity Groups in the Anhalt, Fischer, and Spring Branch 7.5-minute quadrangles, Blanco, Comal, and Kendall Counties, Texas. The hydrostratigraphy was defined based on variations in the amount and type of porosity of each lithostratigraphic unit, which varies depending on the unit’s original depositional environment, lithology, structural history, and diagenesis.

  15. In vivo redox effects of Aspidosperma quebracho-blanco Schltdl., Lantana grisebachii Stuck and Ilex paraguariensis A. St.-Hil. on blood, thymus and spleen of mice.

    Science.gov (United States)

    Canalis, A M; Cittadini, M C; Albrecht, C; Soria, E A

    2014-09-01

    Argentinian native plants Aspidosperma quebracho-blanco, Lantana grisebachii and Ilex paraguariensis are known to have antiinflammatory and antioxidant properties. We demonstrated it in vivo by the redox changes in murine hemolymphatic tissues after infusive extract intake of these plants as revealed in organic trophism, tissue phenolics, hydroperoxides, superoxide, nitrites and gamma-glutamyltranspeptidase in thymus, blood and spleen. A. quebracho-blanco reduced hydroperoxidation in blood and spleen of both sexes, with gamma-glutamyltranspeptidase negativization in lymphatic organs and thymic nitrosative up-regulation. Males have shown increased phenolic content in blood after treatment. L. grisebachii and I. paraguariensis treatment exhibited incomplete antioxidation and oxidative induction in the studied tissues. Different results according to sex were found in redox response to phenolics and their kinetics, with males showing antioxidant effects, whereas females showed oxidative susceptibility. A. quebracho-blanco exhibited protection of murine tissues against oxidation in both sexes and modulation of their trophism, supporting its therapeutic uses in inflammatory diseases. Also, gender had significant influence in phenolic biodistribution and redox response.

  16. Causalidad del precio futuro de la Bolsa de Chicago sobre los precios físicos de maíz blanco en México

    Directory of Open Access Journals (Sweden)

    José Alberto Godínez Placencia

    2007-01-01

    Full Text Available Desde 1993 el gobierno federal mexicano, a través de Apoyos y Servicios a laComercialización Agropecuaria (ASERCA,ha estado utilizando los futuros y opciones de la Bolsa de Chicago de los Estados Unidos como instrumentos paracubrir el riesgo de movimientos adversosde los precios físicos al productor y almayoreo de maíz blanco. El objetivo delpresente trabajo es validar, para el casodel maíz blanco, la pertinencia de usar lascoberturas de los EEUUpara cubrir el riesgo de los precios físicos en México. El conjunto de procedimientos econométricos del vector auto regresivo (función impulso-respuesta, descomposición de la varianza y la causalidad de Granger rechaza la hipótesis de que el precio futuro delmaíz amarillo US #2 de la Bolsa deChicago mantiene una relación de causalidad sobre los precios físicos semanalesde maíz blanco en México para el periodo 1998-2005, por lo que la cobertura internacional no es pertinente.

  17. Effect of hemoglobin powder substituted for fishmeal on growth performance, protein digestibility, and trypsin gene expression in Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Duangrat Chookird

    2010-05-01

    Full Text Available Recent increases in the price and demand of fishmeal, the primary protein source in shrimp feeds, have caused asearch for alternative protein sources. Hemoglobin powder (HE is a by-product produced by separating hemoglobin fromplasma of farm animal un-coagulant blood. HE contains high protein content but low lipid content, and thus has highpotential for fishmeal substitution.A six week feeding trial was carried out to investigate effects of HE substituted for fishmeal protein on growth performance,protein digestibility and trypsin gene expression in Pacific white shrimp (Litopenaeus vannamei. Six diets with0%, 12.5%, 25%, 50%, 75% and 100% of HE replacing fishmeal protein were fed four times daily to six groups of shrimp withan average initial weight of 3.53 g/shrimp. Growth of shrimp decreased with increasing level of HE substitution. Although the12.5% HE substitution caused significantly lower final weight, weight gain, SGR, feed intake, PER and PPV in comparisonwith the control diet, FCR of this diet was not statistically different (p0.05. In-vitro and in-vivo protein digestibility of 12.5% HE substitution were significantly lower than that of the controlgroup in which the trypsin gene expression of shrimp fed 12.5% HE substituted diet was the highest.

  18. Evaluation of Pacific white shrimp (Litopenaeus vannamei) health during a superintensive aquaculture growout using NMR-based metabolomics.

    Science.gov (United States)

    Schock, Tracey B; Duke, Jessica; Goodson, Abby; Weldon, Daryl; Brunson, Jeff; Leffler, John W; Bearden, Daniel W

    2013-01-01

    Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, aquaculture systems are being developed that permit year-round production of farmed shrimp at high densities. These systems are intended to overcome problems of disease susceptibility and of water quality issues from waste products, by operating as essentially closed systems that promote beneficial microbial communities (biofloc). The resulting biofloc can assimilate and detoxify wastes, may provide nutrition for the farmed organisms resulting in improved growth, and may aid in reducing disease initiated from external sources. Nuclear magnetic resonance (NMR)-based metabolomic techniques were used to assess shrimp health during a full growout cycle from the nursery phase through harvest in a minimal-exchange, superintensive, biofloc system. Aberrant shrimp metabolomes were detected from a spike in total ammonia nitrogen in the nursery, from a reduced feeding period that was a consequence of surface scum build-up in the raceway, and from the stocking transition from the nursery to the growout raceway. The biochemical changes in the shrimp that were induced by the stressors were essential for survival and included nitrogen detoxification and energy conservation mechanisms. Inosine and trehalose may be general biomarkers of stress in Litopenaeus vannamei. This study demonstrates one aspect of the practicality of using NMR-based metabolomics to enhance the aquaculture industry by providing physiological insight into common environmental stresses that may limit growth or better explain reduced survival and production.

  19. Evaluation of Pacific white shrimp (Litopenaeus vannamei health during a superintensive aquaculture growout using NMR-based metabolomics.

    Directory of Open Access Journals (Sweden)

    Tracey B Schock

    Full Text Available Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, aquaculture systems are being developed that permit year-round production of farmed shrimp at high densities. These systems are intended to overcome problems of disease susceptibility and of water quality issues from waste products, by operating as essentially closed systems that promote beneficial microbial communities (biofloc. The resulting biofloc can assimilate and detoxify wastes, may provide nutrition for the farmed organisms resulting in improved growth, and may aid in reducing disease initiated from external sources. Nuclear magnetic resonance (NMR-based metabolomic techniques were used to assess shrimp health during a full growout cycle from the nursery phase through harvest in a minimal-exchange, superintensive, biofloc system. Aberrant shrimp metabolomes were detected from a spike in total ammonia nitrogen in the nursery, from a reduced feeding period that was a consequence of surface scum build-up in the raceway, and from the stocking transition from the nursery to the growout raceway. The biochemical changes in the shrimp that were induced by the stressors were essential for survival and included nitrogen detoxification and energy conservation mechanisms. Inosine and trehalose may be general biomarkers of stress in Litopenaeus vannamei. This study demonstrates one aspect of the practicality of using NMR-based metabolomics to enhance the aquaculture industry by providing physiological insight into common environmental stresses that may limit growth or better explain reduced survival and production.

  20. Evaluation of dietary soybean meal as fish meal replacer for juvenile whiteleg shrimp, Litopenaeus vannamei reared in biofloc system

    Directory of Open Access Journals (Sweden)

    Hyeonho Yun

    2017-01-01

    Full Text Available Abstract Different levels of dietary soybean meal (SBM as a fish meal (FM replacer, with and without amino acid supplementation, for whiteleg shrimp, Litopenaeus vannamei reared in the biofloc system was examined in eight weeks of feeding trial. Eight experimental diets consisted of a basal diet with 0% FM replacement by SBM provided in clear sea water without biofloc system (S0SW, four diets replacing FM at 0% (S0, 33% (S33, 67% (S67 and 100% (S100 by SBM, and three diets replacing FM at 33% (S33A, 67% (S67A and 100% (S100A by SBM supplemented with amino acids (methionine and lysine in the seawater biofloc system. Results of water quality analyses showed significantly lower total suspended solids and nitrate for S0SW group than all other treatments. Diets S0 and S33A resulted in higher weight gain and specific growth rate among all groups, with no significant differences with S33 group. In addition, whole-body protein and amino acid compositions of shrimp fed S0SW were lower than most biofloc groups. Haemolymph parameters showed significant differences in total protein, cholesterol and triglyceride between groups S0 and S0SW. Also, superoxide dismutase activity showed a decreasing trend with increasing replacement level. In conclusion, based on these results, SBM could replace up to 33% of FM with or without amino acid supplementation in juvenile whiteleg shrimp diets reared in the biofloc system.

  1. Effects of dietary replacement of fishmeal by mealworm meal on muscle quality of farmed shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Panini, Roseane L; Pinto, Stephanie S; Nóbrega, Renata O; Vieira, Felipe N; Fracalossi, Débora M; Samuels, Richard I; Prudêncio, Elane S; Silva, Carlos P; Amboni, Renata D M C

    2017-12-01

    This study investigated the muscle quality of the shrimp Litopenaeus vannamei fed on a diet containing different proportions of mealworm meal (MW) (0, 25, 50, 75 and 100%) as a substitute for fishmeal, which is the normal diet used in shrimp commercial production. The proximate composition, fatty acid profile, colour and texture of the shrimps were evaluated. Moisture, protein, and ash content of shrimp muscle were not significantly altered when fishmeal was replaced by MW (p>0.05). However, the replacement resulted in a linear increase in lipid content (p<0.05). The fatty acid composition of the experimental diets directly mirrored the fatty acid composition of shrimp muscle. The absence of long-chain polyunsaturated fatty acids in MW composition resulted in a linear decrease in eicosapentaenoic and docosahexaenoic fatty acids in shrimp muscle with increasing levels of MW in the diet. The n-3/n-6 ratio ranged from 0.50 to 0.67. Colour and firmness were unchanged between the treatments. Although the use of MW as a fishmeal substitute in L. vannamei diets has affected the lipid and fatty acid composition of shrimp muscle, from a human nutritional perspective, the lipid content of the shrimps is considered low and the n-3/n-6 ratio remained within the human dietary requirements. Therefore the use of a mealworm diet for shrimp production is a viable alternative to increasingly expensive fishmeal based diets. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Potential Bacillus probiotics enhance bacterial numbers, water quality and growth during early development of white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Nimrat, Subuntith; Suksawat, Sunisa; Boonthai, Traimat; Vuthiphandchai, Verapong

    2012-10-12

    Epidemics of epizootics and occurrence of multiresistant antibiotics of pathogenic bacteria in aquaculture have put forward a development of effective probiotics for the sustainable culture. This study examined the effectiveness of forms of mixed Bacillus probiotics (probiotic A and probiotic B) and mode of probiotic administration on growth, bacterial numbers and water quality during rearing of white shrimp (Litopenaeus vannamei) in two separated experiments: (1) larval stages and (2) postlarval (PL) stages. Forms of Bacillus probiotics and modes of probiotic administration did not affect growth and survival of larval to PL shrimp. The compositions of Bacillus species in probiotic A and probiotic B did not affect growth and survival of larvae. However, postlarvae treated with probiotic B exhibited higher (Pgrowth than probiotic A and controls, indicating Bacillus probiotic composition affects the growth of PL shrimp. Total heterotrophic bacteria and Bacillus numbers in larval and PL shrimp or culture water of the treated groups were higher (Pgrowth and survival of PL shrimp, increased beneficial bacteria in shrimp and culture water and enhanced water quality for the levels of pH, ammonia and nitrite of culture water. Copyright © 2012 Elsevier B.V. All rights reserved.

  3. In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Ana R Álvarez-Sánchez

    2017-05-01

    Full Text Available Objective. The digestibility of specific dsRNA by action of the enzymes of digestive tract of the whiteleg shrimp Litopenaeus vannamei was determined in vitro. Materials and methods. Digestive enzyme activity (amylase, lipase, protease, DNase and RNase was measured in the stomach, digestive gland, and anterior, middle, and posterior intestine of juvenile shrimp, and the digestibility of DNA, RNA and the dsRNA-ORF89, specific to WSSV, was determined by in vitro assays, as well as electrophoretic and densitometric analyses. Results. The highest enzymatic activity was found in the digestive gland: amylase (81.41%, lipase (92.60%, protease (78.20%, DNase (90.85%, and RNase (93.14%. The highest digestive capacity against DNA, RNA, and dsRNA was found in the digestive gland (5.11 ng of DNA per minute, 8.55 ng of RNA per minute, and 1.48 ng dsRNA per minute. Conclusions. The highest digestibility of dsRNA-ORF89, specific to WSSV, was found in the digestive gland, whereas the lowest digestibility was observed in the posterior intestine. This is the first report regarding the digestibility of dsRNA-ORF89 by whiteleg shrimp digestive tract enzymes, with potential therapeutic importance in shrimp culture to prevent WSSV disease through balanced feed.

  4. Optimal content and ratio of lysine to arginine in the diet of Pacific white shrimp, Litopenaeus vannamei

    Science.gov (United States)

    Feng, Zhengfu; Dong, Chaohua; Wang, Linlin; Hu, Yanjiang; Zhu, Wei

    2013-07-01

    The optimal quantity of dietary lysine (Lys) and arginine (Arg), and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated. Coated Lys and Arg were added to a basal diet (37.99% crude protein and 7.28% crude lipid) to provide graded levels of Lys and Arg. The experimental diets contained three Lys levels (2.51%, 2.11%, and 1.70% of total diet), and three Arg levels (1.41%, 1.80%, and 2.21% of total diet) and all combinations of these levels were tested. Pacific white shrimp, with a mean weight of 3.62±0.1 g, were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days. After the feeding trial, the growth performance, survival, feed conversion rate (FCR), body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined. The results show that weight gain (WG), specific growth rate (SGR), FCR, body protein, body Lys and Arg content were significantly affected by dietary Lys and Arg ( P 0.05). Therefore, according to our results, the optimal Lys and Arg quantities in the diet of Pacific white shrimp, L. vannamei, were considered to be 2.11%-2.51% and 1.80%-2.21%, respectively, and the optimal ratio to be 1:0.88-1:1.05.

  5. Dextrose as carbon source in the culture of Litopenaeus vannamei (Boone, 1931 in a zero exchange system

    Directory of Open Access Journals (Sweden)

    Sabrina M Suita

    2015-07-01

    Full Text Available This work compared the use of dextrose and molasses as carbon sources for biofloc development, water quality maintenance, microorganism composition and growth performance of Litopenaeus vannamei juveniles in biofloc technology (BFT. Two treatments, dextrose and molasses, were tested with four replicates each. Carbon was added to achieve a C:N-AT (N-(NH3+NH4+ ratio of 6:1. Physical and chemical water quality variables were monitored daily, and shrimp growth was estimated through periodic biometry. After 30 days, survival, final biomass, and feeding conversion rate (FCR were determined. Dissolved organic carbon, chlorophyll-a, floc volume, total ammonia, nitrite, nitrate and phosphate concentrations, and microorganisms (qualified by groups, were measured every three days. Water quality variables remained within acceptable levels throughout the experimental period, except for nitrite, which reached higher levels than recommended for this species. The use of dextrose resulted in higher water transparency, which influenced the remaining centric diatoms. A superior shrimp performance was observed at this treatment, presumably because of variations on the microbial community. Therefore, it is concluded that the addition of dextrose results in a superior growth performance of L. vannamei when cultured in BFT systems.

  6. TAT improves in vitro transportation of fortilin through midgut and into hemocytes of white shrimp Litopenaeus vannamei

    Science.gov (United States)

    Zhou, Yi; Zhang, Wenbing; Mai, Kangsen; Xu, Wei; Zhang, Yanjiao; Ai, Qinghui; Wang, Xiaojie

    2012-06-01

    Fortilin is a multifunctional protein implicated in many important cellular processes. Since injection of Pm-fortilin reduces shrimp mortality caused by white spot syndrome virus (WSSV), there is potential application of fortilin in shrimp culture. In the present study, in order to improve trans-membrane transportation efficiency, the protein transduction domain of the transactivator of transcription (TAT) peptide was fused to fortilin. The Pichia pastoris yeast expression system, which is widely accepted in animal feeds, was used for production of recombinant fusion protein. Green fluorescence protein (GFP) was selected as a reporter because of its intrinsic visible fluorescence. The fortilin, TAT and GFP fusion protein were constructed. Their trans-membrane transportation efficiency and effects on immune response of shrimp were analyzed in vitro. Results showed that TAT peptide improved in vitro uptake of fortilin into the hemocytes and midgut of Litopenaeus vannamei. The phenoloxidase (PO) activity of hemocytes incubated with GFP-Fortilin or GFP-Fortilin-TAT was significantly increased compared with that in the control without expressed fortilin. The PO activity of hemocytes incubated with 200 μg mL-1 GFP-Fortilin-TAT was significantly higher than that in the group with the same concentration of GFP-Fortilin. Hemocytes incubated with GFP-Fortilin-TAT at all concentrations showed significantly higher nitric oxide synthase (NOS) activity than those in the control or in the GFP-Fortilin treatment. The present in vitro study indicated that TAT fusion protein improved the immune effect of fortilin.

  7. Evaluation of Pacific White Shrimp (Litopenaeus vannamei) Health during a Superintensive Aquaculture Growout Using NMR-Based Metabolomics

    Science.gov (United States)

    Schock, Tracey B.; Duke, Jessica; Goodson, Abby; Weldon, Daryl; Brunson, Jeff; Leffler, John W.; Bearden, Daniel W.

    2013-01-01

    Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, aquaculture systems are being developed that permit year-round production of farmed shrimp at high densities. These systems are intended to overcome problems of disease susceptibility and of water quality issues from waste products, by operating as essentially closed systems that promote beneficial microbial communities (biofloc). The resulting biofloc can assimilate and detoxify wastes, may provide nutrition for the farmed organisms resulting in improved growth, and may aid in reducing disease initiated from external sources. Nuclear magnetic resonance (NMR)-based metabolomic techniques were used to assess shrimp health during a full growout cycle from the nursery phase through harvest in a minimal-exchange, superintensive, biofloc system. Aberrant shrimp metabolomes were detected from a spike in total ammonia nitrogen in the nursery, from a reduced feeding period that was a consequence of surface scum build-up in the raceway, and from the stocking transition from the nursery to the growout raceway. The biochemical changes in the shrimp that were induced by the stressors were essential for survival and included nitrogen detoxification and energy conservation mechanisms. Inosine and trehalose may be general biomarkers of stress in Litopenaeus vannamei. This study demonstrates one aspect of the practicality of using NMR-based metabolomics to enhance the aquaculture industry by providing physiological insight into common environmental stresses that may limit growth or better explain reduced survival and production. PMID:23555690

  8. Comparative Transcriptome Analysis in the Hepatopancreas Tissue of Pacific White Shrimp Litopenaeus vannamei Fed Different Lipid Sources at Low Salinity.

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    Ke Chen

    Full Text Available RNA-seq was used to compare the transcriptomic response of hepatopancreas in juvenile Litopenaeus vannamei fed three diets with different lipid sources, including beef tallow (BT, fish oil (FO, and an equal combination of soybean oil + BT + linseed oil (SBL for 8 weeks at 3 practical salinity unit (psu. A total of 9622 isogenes were annotated in 316 KEGG pathways and 39, 42 and 32 pathways significantly changed in the paired comparisons of FO vs SBL, BT vs SBL, or FO vs BT, respectively. The pathways of glycerolipid metabolism, linoleic acid metabolism, arachidonic acid metabolism, glycerophospholipid metabolism, fatty acid biosynthesis, fatty acid elongation, fatty acid degradation, and biosynthesis of unsaturated fatty acid were significantly changed in all paired comparisons between dietary lipid sources, and the pathways of glycerolipid metabolism, linoleic acid metabolism, arachidonic acid metabolism and glycerophospholipid metabolism significantly changed in the FO vs SBL and BT vs SBL comparisons. These pathways are associated with energy metabolism and cell membrane structure. The results indicate that lipids sources affect the adaptation of L. vannamei to low salinity by providing extra energy or specific fatty acids to change gill membrane structure and control iron balance. The results of this study lay a foundation for further understanding lipid or fatty acid metabolism in L. vannamei at low salinity.

  9. Transcriptome analysis on the exoskeleton formation in early developmetal stages and reconstruction scenario in growth-moulting in Litopenaeus vannamei.

    Science.gov (United States)

    Gao, Yi; Wei, Jiankai; Yuan, Jianbo; Zhang, Xiaojun; Li, Fuhua; Xiang, Jianhai

    2017-04-24

    Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, the transcriptome of the entire developmental process in Litopenaeus vannamei, including nine early developmental stages and eight adult-moulting stages, was sequenced and analysed using Illumina RNA-seq technology. A total of 117,539 unigenes were obtained, with 41.2% unigenes predicting the full-length coding sequence. Gene Ontology, Clusters of Orthologous Group (COG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and functional annotation of all unigenes gave a better understanding of the exoskeleton developmental process in L. vannamei. As a result, more than six hundred unigenes related to exoskeleton development were identified both in the early developmental stages and adult-moulting. A cascade of sequential expression events of exoskeleton-related genes were summarized, including exoskeleton formation, regulation, synthesis, degradation, mineral absorption/reabsorption, calcification and hardening. This new insight on major transcriptional events provide a deep understanding for exoskeleton formation and reconstruction in L. vannamei. In conclusion, this is the first study that characterized the integrated transcriptomic profiles cover the entire exoskeleton development from zygote to adult-moulting in a crustacean, and these findings will serve as significant references for exoskeleton developmental biology and aquaculture research.

  10. Cyanobacteria Community Dynamics and Trophic Status of Intensive Shrimp (Litopenaeus vannamei Farming Pond in Situbondo East Java Indonesia

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    Dian Aliviyanti

    2017-09-01

    Full Text Available The objective of this study was to analyze the dynamics community structure of Cyanobacteria and trophic status in ponds of intensive shrimp culture (Litopenaeus vannamei in Situbondo based on Trophic Diatom Index (TDI.  The ex post facto research was conducted in situ in the hamlet of Pond Mutiara Mas III Klatakan Situbondo East Java Indonesia.  Observation of Cyanobacteria and Diatoms community structure were done every week during four cycles of shrimp farming ponds.  Cycle of shrimp farming ponds is the times for enlargement process of the shrimps from seed to mature which ranges from 90-120 days.  The dependent variables were the density of Cyanobacteria and Diatoms community, as well as chemical parameters nitrite and orthophosphate.  Trophic status was determined from TDI be counted from data of diatom density.  All of the data were then classified using cluster and biplot analysis program PAST Ver. 3.11 to discribe the profile of ecosystem quality.  The research results showed that there were four taxa of Cyanobacteria during farming cycle which the highest density was found from the genus of Oscillatoria.  Based on the value of TDI, we found that the trophic status of water in shrimps pond during the production process was eutrophic until hyper-eutrophic.  The water quality was decreases along with the length of incubation time.

  11. Application of wet waste from shrimp ( Litopenaeus vannamei) with or without sea mud to feeding sea cucumber ( Stichopus monotuberculatus)

    Science.gov (United States)

    Chen, Yanfeng; Hu, Chaoqun; Ren, Chunhua

    2015-02-01

    In the present study, the applicability of the wet waste collected from shrimp ( Litopenaeus vannamei) to the culture of sea cucumber ( Stichopus monotuberculatus) was determined. The effects of dietary wet shrimp waste on the survival, specific growth rate (SGR), fecal production rate (FPR), ammonia- and nitrite-nitrogen productions of sea cucumber were studied. The total organic matter (TOM) level in the feces of sea cucumber was compared with that in corresponding feeds. Diet C (50% wet shrimp waste and 50% sea mud mash) made sea cucumber grow faster than other diets. Sea cucumber fed with either diet D (25% wet shrimp waste and 75% sea mud mash) or sole sea mud exhibited negative growth. The average lowest total FPR of sea cucumber occurred in diet A (wet shrimp waste), and there was no significant difference in total FPR between diet C and diet E (sea mud mash) ( P > 0.05). The average ammonia-nitrogen production of sea cucumber in different diet treatments decreased gradually with the decrease of crude protein content in different diets. The average highest nitrite-nitrogen production occurred in diet E treatment, and there was no significant difference in nitrite-nitrogen production among diet A, diet B (75% wet shrimp waste and 25% sea mud mash) and diet C treatments ( P > 0.05). In each diet treatment, the total organic matter (TOM) level in feces decreased to different extent compared with that in corresponding feeds.

  12. Effect of shrimp ( Litopenaeus vannamei) farming waste on the growth, digestion, ammonium-nitrogen excretion of sea cucumber ( Stichopus monotuberculatus)

    Science.gov (United States)

    Chen, Yanfeng; Luo, Peng; Hu, Chaoqun; Ren, Chunhua

    2015-06-01

    In this study, specific growth rate (SGR), ingestion rate (IR), food conversion ratio (FCR), apparent digestion ratio (ADR) and ammonium-nitrogen excretion were determined for sea cucumber ( Stichopus monotuberculatus) reared in plastic containers (70 L; 4 containers each diet treatment). Sea cucumbers were fed with five diets containing different amounts of farming waste from shrimp ( Litopenaeus vannamei) (100%, 75%, 50%, 25% and 0) and a formulated compound (20% sea mud and 80% powdered algae). Sea cucumbers grew faster when they were fed with diet D (25% shrimp waste and 75% formulated compound) than those fed with other diets. Although IR value of sea cucumber fed with diet A (shrimp waste) was higher than those fed with other diets, both the lowest SGR and the highest FCR occurred in this diet group. The highest and the lowest ADR occurred in diet E (formulated compound) and diet A group, respectively, and the same to ammonium-nitrogen excretion. The contents of crude protein, crude lipid and total organic matter (TOM) in feces decreased in comparison with corresponding diets. In the feces from different diet treatments, the contents of crude protein and TOM increased gradually as the contents of crude protein and TOM in diets increased, while crude lipid content decreased gradually as the crude lipid content in diets increased.

  13. White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing

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    Yu-Yuan Chen

    2015-06-01

    Full Text Available White shrimp Litopenaeus vannamei immersed in seawater (35‰ containing Gracilaria tenuistipitata extract (GTE at 0 (control, 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen, and immune parameters including hyaline cells (HCs, granular cells (GCs, including semi-granular cells, total hemocyte count (THC, phenoloxidase (PO activity, respiratory bursts (RBs, superoxide dismutase (SOD activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP, peroxinectin (PX, cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

  14. Daily cycle and body characteristics of mating Litopenaeus vannamei shrimps (Decapoda: Penaeidae) in the wild off southern Sinaloa, Mexico.

    Science.gov (United States)

    Calderón-Pérez, J A; Rendón-Rodríguez, S; Solís-Ibarra, R

    2007-03-01

    Mating behavior has been described for very few species of penaeoid shrimps. We describe some of the environmental conditions under which mating of Litopenaeus vannamei took place in the ocean, as inferred by the presence of attached spermatophores on the thelycum of females, combined with the presence of mature males with empty ampulla terminalis, both evidence of recent copulation. Out of a total of twelve 24 hr samplings on board the research vessel B/O "EL PUMA", one was selected to examine mating. There were four periods of observation. The highest frequency of females with attached spermatophores were found during the daytime whilst the lowest was registered during the night. Females with attached spermatophore were mostly at stages IV and V of ovarian maturity and fell within the 30-50 mm of C.L. range. Copulating male size ranged between 30 and 40 mm of C.L. and there was a close relation between the percentage of mating females and males. Male to female ratio varied throughout the sampling period but it was never 1:1. The female mean size was, in all cases, bigger than the male mean size. Among the environmental factors, salinity and oxygen varied very slightly throughout the sampling period; temperature variation was more pronounced and those changes were attributed to the tidal oscillation.

  15. Application of Edible Films Containing Oregano (Origanum vulgare) Essential Oil on Queso Blanco Cheese Prepared with Flaxseed (Linum usitatissimum) Oil.

    Science.gov (United States)

    Gurdian, Cristhiam; Chouljenko, Alexander; Solval, Kevin Mis; Boeneke, Charles; King, Joan M; Sathivel, Subramaniam

    2017-06-01

    Fortification of queso blanco (QB) with flaxseed oil (FO) containing omega-3 polyunsaturated fatty acids may provide a functional food with health benefits such as improved cell, brain, and retina functionality, and protection against cardiovascular and immune-inflammatory diseases. However, QB experiences a short shelf life because of the early development of yeasts and molds and addition of FO may increase susceptibility to lipid oxidation. Oregano essential oil (OEO) is known for its antimicrobial and antioxidant properties, but due to its intense flavor compounds it may not be suitable for direct incorporation into QB. Thus, incorporation of OEO into an edible film prepared with whey protein isolate (WPI) may improve the shelf life of QB. Scanning electron microscopy (SEM) micrographs revealed that FO was successfully retained by the cheese after homogenization. The thiobarbituric-acid-reactive-substances (TBARS) and yeast and mold counts (YMC) of the wrapped cheeses were analyzed during 60 d of refrigerated storage. The oxidation rate increased significantly for nonwrapped QB containing FO (QBFO) during storage, however wrapping with WPI edible films containing OEO (WOF) significantly limited lipid oxidation and prevented growth of yeasts and molds. This study demonstrated the antioxidant and antimicrobial properties of WOF for preservation of QBFO during refrigerated storage. © 2017 Institute of Food Technologists®.

  16. Influence of root-knot nematode infestation on antioxidant enzymes, chlorophyll content and growth in Pogostemon cablin (Blanco) Benth.

    Science.gov (United States)

    Bhau, B S; Borah, Bitupon; Ahmed, Reshma; Phukon, P; Gogoi, Barbi; Sarmah, D K; Lal, M; Wann, S B

    2016-04-01

    Plants adapt themselves to overcome adverse environmental conditions, and this involves a plethora of concurrent cellular activities. Physiological experiments or metabolic profiling can quantify this response. Among several diseases of Pogostemon cablin (Blanco) Benth. (Patchouli), root-knot nematode infection caused by Meloidogyne incognita (Kofoid and White) Chitwood causes severe damage to the plant and hence, the oil production. In the present study, we identified M. incognita morphologically and at molecular level using sequenced characterized amplified region marker (SCAR). M. incognita was artificially inoculated at different levels of second stage juveniles (J₂) to examine the effect on Patchouli plant growth parameters. Peroxidase and polyphenol oxidase enzyme activity and changes in the total phenol and chlorophyll contents in M. incognita was also evaluated in response to infection. The results have demonstrated that nematode infestation leads to increased peroxidase activities in the leaves of the patchouli plants and thereby, increase in phenolic content as a means of defence against nematode infestation. Chlorophyll content was also found decreased but no changes in polyphenol oxidase enzyme activity.

  17. Identification of protoplast-isolation responsive microRNAs in Citrus reticulata Blanco by high-throughput sequencing.

    Science.gov (United States)

    Xu, Xiaoyong; Xu, Xiaoling; Zhou, Yipeng; Zeng, Shaohua; Kong, Weiwen

    2017-01-01

    Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts. MicroRNAs (miRNAs) play important roles in plant growth, development and stress responses. However, the underlying mechanism of miRNAs involved in the protoplast totipotency remains unclear. In this study, high-throughput sequencing technology was used to sequence two populations of small RNA from calli and callus-derived protoplasts in Citrus reticulata Blanco. A total of 67 known miRNAs from 35 families and 277 novel miRNAs were identified. Among these miRNAs, 18 known miRNAs and 64 novel miRNAs were identified by differentially expressed miRNAs (DEMs) analysis. The expression patterns of the eight DEMs were verified by qRT-PCR. Target prediction showed most targets of the miRNAs were transcription factors. The expression levels of half targets showed a negative correlation to those of the miRNAs. Furthermore, the physiological analysis showed high levels of antioxidant activities in isolated protoplasts. In short, our results indicated that miRNAs may play important roles in protoplast-isolation response.

  18. Effects of Combined Heat and Preservative Treatment on Storability of Ponkan Fruit (Citrus reticulata Blanco cv. Ponkan during Postharvest Storage

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    Dandan Tang

    2017-01-01

    Full Text Available Heat treatment and preservative application have been widely used during postharvest storage of many fresh products, but the effect of their combination on citrus storage has rarely been investigated. In this study, the optimal heat treatment (HT conditions and HT combined with preservative treatment were investigated for Ponkan fruit (Citrus reticulata Blanco cv. Ponkan storage. Results indicated that HT at 55°C for 20 s can significantly reduce the decay rate of Ponkan fruit, and a combination of HT and 25% of the preservative dosage used in production of iminoctadine tris (albesilate, 2,4-dichlorophenoxyacetic acid, and imazalil significantly reduced the decay rate without affecting fruit quality. In addition, the increased fiber contents in fruit receiving the HT combined with preservative treatments may be a response preventing fungus infection and enhancing fruit storability and resistance. The above results suggested that the combination of HT and 25% of the preservative production dosage was optimal for controlling Ponkan fruit decay during storage.

  19. Rho GTPasas como blancos terapéuticos relevantes en cáncer y otras enfermedades humanas

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    Pablo Lorenzano Menna

    2010-12-01

    Full Text Available Las Rho GTPasas son una familia de proteínas clave en la transmisión de señales provenientes del exterior celular hacia efectores intracelulares tanto citoplasmáticos como nucleares. En los últimos año ha habido un desarrollo vertiginoso de múltiples herramientas genéticas y farmacológicas, lo que ha permitido establecer de manera mucho más precisa las funciones específicas de estas proteínas. El objetivo de la presente revisión es hacer foco en las múltiples funciones celulares reguladas por las Rho GTPasas, describiendo en detalle el mecanismo molecular involucrado. Se discute además la participación de estas proteínas en diversas enfermedades humanas haciendo énfasis en su vinculación con el cáncer. Por último, se hace una actualización detallada sobre las estrategias terapéuticas en experimentación que tienen a las Rho GTPasas como blancos moleculares.

  20. Change of mitotic behavior and ultra structure of 'Fuju' (Citrus reticulata Blanco) stem-apex clones after space flight

    International Nuclear Information System (INIS)

    Wu Rujian; Huang Jinghao; Wen Shouxing; Cai Zijian; Luo Tuyan; Chen Liangfeng; Wang Zhouwen

    2011-01-01

    By using conventional squash stain technique and ultrathin sectioning technique, the effects of space flight on mitotic behavior and ultrastructure were studied in the shoot apical meristem of 'Fuju' (Citrus reticulata Blanco), which had been carried by China's seed-breeding satellite, Shijian-8. The results showed that space flight had effect on the mutagenesis of stem-apical meristem. Abnormal mitosis with various degrees had been detected in 13 mutant clones, of which mitotic aberrations in clone '08004' were significantly higher than the others. The aberration rate of numerical abnormalities of chromosomes at metaphase, lagging chromosome, micronucleus, C-spindle, S-spindle and polyarch spindle in the clone '08004' was 0.34%, 0.669%, 0.86%, 0.17%, 1.20% and 1.03%, respectively. The ultrastructure of mesophyll cell in most clones was unchanged, but nucleus chromatin agglutination, chloroplast thylakoid disintegrated, autophagosome appeared, cell vacuolated, plasmolysis and the formation of apoptotic body were found in the clone '08004', suggesting that programmed cell death (PCD) Nevertheless, no change in the mitochondrial structure was observed until terminal phase of PCD. (authors)

  1. Caracterización cariotípica en mitosis y meiosis del robalo blanco Centropomus undecimalis (Pisces: Centropomidae

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    Lenin Arias-Rodriguez

    2011-06-01

    Full Text Available El robalo blanco Centropomus undecimalis, vive en hábitats marinos, salobres y dulceacuícolas en el océano Atlántico occidental, incluyendo el golfo de México. La especie, es económicamente importante en varias localidades, no obstante los estudios sobre su biología y genética son hasta el momento pocos. El presente estudio tiene como propósito, la caracterización citogenética de especímenes recolectados en el municipio de Paraíso, Tabasco, México. Cinco hembras y ocho machos fueron procesados por técnicas citológicas convencionales para la obtención de preparaciones cromosómicas de buena calidad para elaborar el cariotipo. Los resultados del análisis del tejido del riñón, mostraron que 85.1% de 288 mitosis tienen 2n=48 cromosomas y 52.8% de 104 meiosis exhiben el número haploide de 1n=24. El cariotipo diploide mostro 48 cromosomas monorrámeos de tipo telocéntrico (T. No se observó heteromorfismo cromosómico entre hembras y machos. El cariotipo diploide fue similar a los observados en la mayoría de peces marinos.

  2. Acute toxicity of pyrazosulfuron-ethyl and permethrin to juvenile Litopenaeus vannamei =Toxicidade aguda de pirazossulfurom-etílico e permethrin em juvenis de camarão branco Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Giovanni Lemos de Mello

    2011-01-01

    Full Text Available The objective of this study was to determine the LC50 (96h of two pesticides: SiriusR 250 SC herbicide of the pyrazosulfuron-ethyl group, and TalcordR insecticide of the permethrin group, on juvenile Litopenaeus vannamei. Shrimp total hemocyte count (THC was also determined as an indication of physiological alterations caused by the pesticides. Juvenile shrimp (5.0 } 0.5 g were exposed to the following concentrations: 0, 0.1, 1.0, 10, 100 and 1000 ƒÊg L-1 SiriusR 250 SC; and 0, 0.001, 0.01, 0.1, 1.0 and 10 ƒÊg L-1 TalcordR. The TalcordR LC50 (96h was of 0.00933 ƒÊg L-1 or 9.33 ng L-1. There were no significant changes in the THC between control and test groups. No SiriusR 250 SC concentrations tested killed more than 50%of the shrimp; therefore, the herbicide was considered not toxic to the juveniles. However, the THC showed significant differences between the control and test groups, suggesting sublethal effects to L. vannamei juveniles. According to the results, the insecticide TalcordR is highly lethal for L. vannamei and the herbicide SiriusR 250 SC was not lethal in the concentrations tested but showed sublethal effects as lower THC. The results demonstrate the risks involved in farming L. vannamei shrimp near rice cultures where these pesticides are routinely used.O objetivo deste trabalho foi determinar a CL50 (96h dos agroquimicos SiriusR 250 SC, herbicida a base de pirazossulfurom-etilico, e TalcordR, inseticida a base de permethrin, em juvenis de Litopenaeus vannamei, bem como avaliar possiveis alteracoes fisiologicas por meio da contagem total de hemocitos (CTH dos camaroes. Juvenis de L. vannamei (5,0 } 0,5 g foram expostos as seguintes concentracoes dos agroquimicos: SiriusR 250 SC, 0; 0,1; 1; 10; 100 e 1.000 ƒÊg L-1, e TalcordR, 0; 0,001; 0,01; 0,1; 1 e 10 ƒÊg L-1. A CL50 (96h do inseticida TalcordR foi de 0,00933 ƒÊg L-1 ou 9,33 ng L-1. Nao houve alteracoes significativas da CTH entre as medias dos grupos

  3. Efeito do tipo de sedimento na eficiência alimentar, crescimento e sobrevivência de Litopenaeus vannamei (Boone, 1931 = Effect of sediment type on feed efficiency, growth and survival of Litopenaeus vannamei (Boone, 1931

    Directory of Open Access Journals (Sweden)

    Daniele Bezerra dos Santos

    2011-09-01

    Full Text Available Este estudo avaliou o desempenho zootécnico do camarão marinho Litopenaeus vannamei em diferentes tipos de substratos não consolidados. Juvenis (0,97 ± 0,27 g foram aclimatados na densidade de 52 m-2, em 21 caixas de polietileno com biofiltros individuais, emum sistema fechado de filtração contínua, sendo submetidos aos tratamentos: 1 A = 25% silte + 25% argila + 25% areia muito fina + 25% areia fina; 2 B = 50% areia fina + 50% areia muito fina e 3 Controle = sem substrato, em um delineamento experimental completamente casualizado, com três tratamentos e sete repetições cada. Os animais foram alimentados com 12% da biomassa/dia com ração peletizada (35% de proteína bruta, ofertada em bandejas e parceladaem duas ofertas diárias (8 e 16h. O estudo teve duração de 48 dias, sendo avaliados: consumo aparente de ração, eficiência alimentar, taxa de crescimento específico, sobrevivência e ganho de peso. Foram acompanhados diariamente salinidade, pH, amônia e temperatura da água. A análise estatística dos dados se deu por meio dos testes Anova ou Kuskal-Wallis, em função da sua parametricidade. Não foram observadas diferenças significativas com relação aos fatores avaliadosnas diferentes granulometrias de sedimento testadas, indicando que estas não exerceram influência sobre o desempenho zootécnico de L. vannamei juvenil.This study aimed to evaluate the zootechnical performance of Litopenaeus vannamei shrimp at different types of unconsolidated substrate. Juvenile shrimp (0.97 ± 0.27 g were acclimatized, in a stocking density of 52 m-2, to 21 polyethylene boxes (50 L connected individually to biofilters in a closed water exchange system. The animals were submitted to the treatments: 1 A = 25% silt + 25% clay + 25% very fine sand + 25% fine sand; 2 B = 50% fine sand + 50% very fine sand and 3 Control = no sediment, with seven replications each. Photoperiod was set on 12L: 12D. Shrimps were fed twice a day (at08:00 and

  4. Oxygen consumption of Litopenaeus vannamei juveniles in heterotrophic medium with zero water exchange Consumo de oxigênio de juvenis de Litopenaeus vannamei em meio heterotrófico sem renovação de água

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    Luis Vinatea

    2009-05-01

    Full Text Available This work aimed at determining the dissolved oxygen consumption rate of Litopenaeus vannamei juveniles maintained in a microbial biofloc raceway system at high density with no aeration. Three 4 L bottles were filled for each treatment, sealed hermetically, and placed in an enclosed greenhouse raceway system. Four shrimp (13.2±1.42 g were assigned to two sets of the bottles, which underwent the following treatments: light conditions with no shrimp; dark conditions with no shrimp; light conditions with shrimp; and dark conditions with shrimp. Dissolved oxygen content was measured every 10 min for 30 min. A quadratic behavior was observed in dissolved oxygen concentration over time. Significant differences for oxigen consumption were observed only at 10 and 20 min between shrimp maintained in the dark and those under light conditions. At 10 min, a higher value was observed in shrimp maintained under light, and at 20 min, in the dark. Significant differences between 10 and 20 min and between 10 and 30 min were observed when oxygen consumption was analyzed over time in the presence of light. Under dark conditions there were significant differences only between 20 and 30 min. Lethal oxygen concentration (0.65 mg L-1 would be reached in less than one hour either under light or dark conditions with no aeration.O objetivo deste trabalho foi determinar o consumo de oxigênio dissolvido (OD de juvenis de Litopenaeus vannamei mantidos em sistema de cultivo de bioflocs bacterianos em alta densidade e ausência de aeração. Três garrafas de 4 L foram preenchidas para cada tratamento, fechadas hermeticamente e colocadas em sistema de cultivo fechado. Quatro camarões (13,2±1,42 g foram colocados em dois dos conjuntos de garrafas. Os tratamentos aplicados foram: luminosidade, sem camarões; escuro, sem camarões; luminosidade, com camarões; escuro, com camarões. A concentração de oxigênio dissolvido foi determinada a cada 10 min durante 30 min. Foi

  5. The lower mass function of the young open cluster Blanco 1: from 30 MJup to 3 M⊙

    Science.gov (United States)

    Moraux, E.; Bouvier, J.; Stauffer, J. R.; Barrado y Navascués, D.; Cuillandre, J.-C.

    2007-08-01

    Aims:We performed a deep wide field optical survey of the young (~ 100-150 Myr) open cluster Blanco 1 to study its low mass population well down into the brown dwarf regime and estimate its mass function over the whole cluster mass range. Methods: The survey covers 2.3 square degrees in the I and z-bands down to I≃ z≃ 24 with the CFH12K camera. Considering two different cluster ages (100 and 150 Myr), we selected cluster member candidates on the basis of their location in the (I,I-z) CMD relative to the isochrones, and estimated the contamination by foreground late-type field dwarfs using statistical arguments, infrared photometry and low-resolution optical spectroscopy. Results: We find that our survey should contain about 57% of the cluster members in the 0.03-0.6~M⊙ mass range, including 30-40 brown dwarfs. The candidate's radial distribution presents evidence that mass segregation has already occured in the cluster. We took it into account to estimate the cluster mass function across the stellar/substellar boundary. We find that, between 0.03~M⊙ and 0.6~M⊙, the cluster mass distribution does not depend much on its exact age, and is well represented by a single power-law, with an index α=0.69± 0.15. Over the whole mass domain, from 0.03 M⊙ to 3 M⊙, the mass function is better fitted by a log-normal function with m0=0.36± 0.07~M⊙ and σ=0.58±0.06. Conclusions: Comparison between the Blanco 1 mass function, other young open clusters' MF, and the galactic disc MF suggests that the IMF, from the substellar domain to the higher mass part, does not depend much on initial conditions. We discuss the implications of this result on theories developed to date to explain the origin of the mass distribution. Based on observations obtained at the Canada-France-Hawaii Telescope (CFHT) which is operated by the National Research Council of Canada, the Institut National des Sciences de l'Univers of the Centre National de la Recherche Scientifique of France

  6. Serological and molecular studies of a novel virus isolate causing yellow mosaic of Patchouli [Pogostemon cablin (Blanco) Benth].

    Science.gov (United States)

    Zaim, Mohammad; Ali, Ashif; Joseph, Jomon; Khan, Feroz

    2013-01-01

    Here we have identified and characterized a devastating virus capable of inducing yellow mosaic on the leaves of Patchouli [Pogostemon cablin (Blanco) Benth]. The diagnostic tools used were host range, transmission studies, cytopathology, electron microscopy, serology and partial coat protein (CP) gene sequencing. Evidence from biological, serological and sequence data suggested that the causal virus belonged to genus Potyvirus, family Potyviridae. The isolate, designated as Patchouli Yellow Mosaic Virus (PaYMV), was transmitted through grafting, sap and the insect Myzus persicae (Sulz.). Flexuous rod shaped particles with a mean length of 800 nm were consistently observed in leaf-dip preparations from natural as well as alternate hosts, and in purified preparation. Cytoplasmic cylindrical inclusions, pinwheels and laminar aggregates were observed in ultra-thin sections of infected patchouli leaves. The purified capsid protein has a relative mass of 43 kDa. Polyclonal antibodies were raised in rabbits against the coat protein separated on SDS - PAGE; which were used in ELISA and western blotting. Using specific antibodies in ELISA, PaYMV was frequently detected at patchouli plantations at Lucknow and Bengaluru. Potyvirus-specific degenerate primer pair (U335 and D335) had consistently amplified partial CP gene from crude preparations of infected tissues by reverse transcription polymerase chain reaction (RT-PCR). Comparison of the PCR product sequence (290 bp) with the corresponding regions of established potyviruses showed 78-82% and 91-95% sequence similarity at the nucleotide and amino acid levels, respectively. The results clearly established that the virus under study has close homology with watermelon mosaic virus (WMV) in the coat protein region and therefore could share a common ancestor family. Further studies are required to authenticate the identity of PaYMV as a distinct virus or as an isolate of WMV.

  7. Low Temperature Induced Changes in Citrate Metabolism in Ponkan (Citrus reticulata Blanco cv. Ponkan Fruit during Maturation.

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    Qiong Lin

    Full Text Available Citrate is the most important organic acid in citrus fruit, and its concentration in fruit cells is regulated mainly by the balance between synthesis and degradation. Ponkan (Citrus reticulate Blanco cv. Ponkan is one of the major citrus cultivars grew in China, and the fruit are picked before fully mature to avoid bad weather. Greenhouse production is widely used to prolong the maturation period and improve the quality of Ponkan fruit by maintaining adequate temperature and providing protection from adverse weather. In this research, Ponkan fruit cultivated in either a greenhouse or open field were used to investigate differences in the expression of genes related to citrate metabolism during maturation in the two environments. The citrate contents were higher in open field fruit, and were mainly correlated with expressions of CitPEPCs, CitCSs, CitAco3 and CitGAD4, which were significantly increased. In addition, the impacts of low temperature (LT and water stress (WS on citrate metabolism in Ponkan were investigated during fruit maturation. The citrate contents in LT fruit were significantly increased, by between 1.4-1.9 fold, compared to the control; it showed no significant difference in fruit with water stress treatment compared to the control fruit. Furthermore, the expressions of CitPEPCs, CitCSs, CitAco3 and CitGAD4 were significantly increased in response to LT treatment, but showed no significant difference in WS compared to the control fruit. Thus, it can be concluded that low temperature may be the main factor influencing citrate metabolism during maturation in Ponkan fruit.

  8. Isolation of Potential Bacteria as Inoculum for Biofloc Formation in Pacific Whiteleg Shrimp, Litopenaeus vannamei Culture Ponds.

    Science.gov (United States)

    Kasan, Nor Azman; Ghazali, Nurarina Ayuni; Ikhwanuddin, Mhd; Ibrahim, Zaharah

    2017-01-01

    A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as "Biofloc technology". Microbial communities in biofloc aggregates are responsible in eliminating water exchange and producing microbial proteins that can be used as supplemented feed for L. vannamei. This study aimed to isolate and identify potential bioflocculant-producing bacteria to be used as inoculum for rapid formation of biofloc. For the purpose of this study, bacterial communities during 0, 30 and 70 days of culture (DOC) of L. vannamei grow-out ponds were isolated and identified through phenotypic and 16S rDNA sequences analysis. Phylogenetic relationships between isolated bacteria were then evaluated through phylogenetic tree analysis. One-way analysis of variance (ANOVA) was used to compare the differences of microbial communities at each DOC. Out of 125 bacterial isolates, nine species of bacteria from biofloc were identified successfully. Those bacteria species were identified as Halomonas venusta, H. aquamarina, Vibrio parahaemolyticus, Bacillus infantis, B. cereus, B. safensis, Providencia vermicola, Nitratireductor aquimarinus and Pseudoalteromonas sp., respectively. Through phylogenetic analysis, these isolates belong to Proteobacteria and Firmicutes families under the genera of Halomonas sp., Vibrio sp., Bacillus sp., Providencia sp., Nitratireductor sp. and Pseudoalteromonas sp. In this study, bioflocculant-producing bacteria were successfully identified which are perfect candidates in forming biofloc to reduce water pollution towards a sustainable aquaculture industry. Presence of Halomonas sp. and Bacillus sp. in all stages of biofloc formation reinforces the need for new development regarding the ability of these species to be used as inoculum in forming biofloc rapidly.

  9. Impact of the O2 concentrations on bacterial communities and quality of modified atmosphere packaged Pacific white shrimp (Litopenaeus vannamei).

    Science.gov (United States)

    Qian, Yun-Fang; Yang, Sheng-Ping; Xie, Jing; Xiong, Qing; Gao, Zhi-Li

    2013-12-01

    The importance of spoilage-related bacteria in fresh Pacific white shrimp (Litopenaeus vannamei) under different modified atmospheres (MAs) at 4 °C and the effect of O2 were demonstrated in the current study. The changes of bacterial communities in MA-packed shrimp during cold storage were studied by a combined method of plate counts with isolation and identification. Three gas mixtures were applied: 80% CO2 /5% O2 /15% N2, 80% CO2 /10% O2 /10% N2 and 80% CO2 /20% O2, and unsealed packages of shrimp were used as the control. In addition, the TVB-N, pH, whiteness index, and sensory scores were also determined to evaluate the quality changes of shrimp. MA packaging effectively inhibited the increase of total psychrotrophic bacteria counts and H2 S-producing bacteria counts by about 1.7 and 2.1 log cycles, respectively. The growth of Gram-negative spoilage bacteria in shrimp, including Shewanella spp., Aeromonas spp., and Pseudomonas spp., was inhibited by MA packaging, but the growth rate of Gram-positive bacteria such as lactic acid bacteria (LAB) and Brochothrix spp. were less affected by MA as effectively as Gram-negative bacteria. In comparison with the MA-packaged samples, the counts of H2 S producers in shrimp under a CO2 -enriched atmosphere with 20% O2 were slightly lower than the count in samples under an atmosphere with 5% O2 . However, MA with 20% O2 led to higher concentrations of TVB-N, and lower whiteness values and sensory scores. The shelf life of shrimp under 80% CO2 /10% O2 /10% N2 has been prolonged by > 6 d in comparison with the control according to the sensory scores. © 2013 Institute of Food Technologists®

  10. Monitoring of inland waters for culturing shrimp Litopenaeus vannamei: application of a method based on survival and chemical composition.

    Science.gov (United States)

    Valencia-Castañeda, G; Millán-Almaraz, M I; Fierro-Sañudo, J F; Fregoso-López, M G; Páez-Osuna, F

    2017-08-01

    This study evaluated the suitability of 21 inland waters (16 well waters and 5 surface waters) from Northwest Mexico via short- (48 h) and medium-term (28 days) tests using postlarvae (PL18) of Litopenaeus vannamei. In the short test, survival was assessed at 48 h after shrimp were placed in groups of 10 postlarvae into 2-L containers of inland water, to which they had been previously acclimated. The second, medium-term test consisted of four replicates with 10 postlarvae, and each group was placed in 15-L containers with the treatment water. Weights (initial and final) and survival were evaluated weekly for 28 days. In those waters for which the short test was positive and the medium-term test was negative and which also had a deficiency of potassium and/or magnesium, a third test was conducted. These last waters were supplemented with salts, and the shrimp survival and weights (initial and final) were recorded for 28 days. The water samples from San Jose, Mochicahui, Sinaloa River, Caimanero inner Lagoon, La Pipima, Campo Santa Fe, Escopama, and Fitmar had >60% survival in the short test. The Caimanero inner Lagoon water had the highest survival (87.5 ± 9.6%) and final mean weight (201.3 ± 86.2 mg). In the third test, it was found that shrimp in the water from La Pipima, Campo Santa Fe, and Fitmar exhibited 100% survival for 2 weeks. Finally, in this work, a decision tree to evaluate the suitability of low-salinity water for shrimp farming was proposed, which can be applied in other regions.

  11. Effects of ammonia-N stress on metabolic and immune function via the neuroendocrine system in Litopenaeus vannamei.

    Science.gov (United States)

    Cui, Yanting; Ren, Xianyun; Li, Jian; Zhai, Qianqian; Feng, Yanyan; Xu, Yang; Ma, Li

    2017-05-01

    The purpose of this study was to evaluate the immunological responses, such as phenoloxidase (PO), antibacterial, and bacteriolytic activities, and metabolic variables, such as oxyhemocyanin, lactate, and glucose levels, of Litopenaeus vannamei exposed to ambient ammonia-N at 0, 2.5, 5, 7.5, and 10 mg/L for 0, 3, 6, 12, 24, and 48 h, and determine the effects of the eyestalk hormone on the metabolic and immune functions of unilateral eyestalk-ablated L. vannamei exposed to ambient ammonia-N at 10 mg/L. The actual concentrations of the control and test solutions were 0.04, 2.77, 6.01, 8.30, and 11.36 mg/L for ammonia-N and 0.01, 0.15, 0.32, 0.44, and 0.60 mg/L for NH 3 -N (unionized ammonia). The results showed a significant decrease in the PO, antibacterial, and bacteriolytic activities in the plasma as well as a significant increase in the glucose and lactate levels and decreased oxyhemocyanin levels in the hemolymph of L. vannamei exposed to elevated ammonia-N levels. These findings indicated that L. vannamei exposed to ammonia-N might demonstrate weakened metabolic and immunological responses. Moreover, eyestalk removal caused a dramatic decrease in PO, antibacterial, and bacteriolytic activities, which indicated that the eyestalk hormone in L. vannamei exhibited a higher immune response due to the induction of protective mechanisms against ammonia-N stress. Eyestalk removal also caused a dramatic decrease in glucose and lactate levels, suggesting that the eyestalk hormone is involved in glucose metabolism to meet the energy requirements under ammonia-N stress conditions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

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    Irasema E Luis-Villaseñor

    2015-09-01

    Full Text Available The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5 CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus and negative (not injected controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.

  13. Comparison of white spot syndrome virus infection resistance between Exopalaemon carinicauda and Litopenaeus vannamei under different salinity stresses

    Science.gov (United States)

    Ge, Qianqian; Yu, Ge; Sun, Ming; Li, Jitao; Li, Jian

    2017-12-01

    Exopalaemon carinicauda is one of the important economic shrimp species in China, and can tolerate a wide range of salinities. However, its disease resistance remains to be unclear in comparison with other shrimp species under salinity stress. In this study, the resistance to white spot syndrome virus (WSSV) of E. carinicauda and Litopenaeus vannamei was determined by comparing their hemocyanin (Hc) and phenoloxidase (PO) activities under different salinity stresses. In E. carinicauda, the PO activity and Hc gene transcript abundance showed a coherent pattern of increase and decrease while Hc content showed a slightly decrease with Vibrio anguillarum and WSSV infections. For both E. carinicauda and L. vannamei under salinity stress, the PO activity showed a positive correlation with the salinity while the Hc content and expression level of its gene increased significantly in salinities of 5, 15 and 25 g L-1. The survival rate of E. carinicauda with WSSV infection was higher than that of L. vannamei in the first 24 h under different salinity stresses. Drastic mortality of E.carinicauda and L. vannamei appeared at 48 h and 3 h post-injection, respectively. Furthermore, compared with L. vannamei, E. carinicauda displayed higher PO activity, Hc content and abundance of Hc gene mRNA. The results collectively indicated that Hc and PO have obviously functional connection in resisting pathogens and tolerating salinity stress, and PO activity and Hc gene mRNA abundance may reflect the resistance of shrimp to disease. E. carinicauda has higher level of immune potential than L. vannamei, suggesting its greater capacity in resisting pathogens under salinity stresses.

  14. Evaluación microbiológica y sensorial de fermentados de pozol blanco, con cacao (Theobroma cacao y coco (Cocos nucifera

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    Román Jiménez Vera

    2010-07-01

    Full Text Available El pozol es una bebida de maíz que se consume en el sureste de México y en algunos países de Centroamérica. Se puede consumir recién elaborado o fermentado. Tradicionalmente se consume solo (pozol blanco, aunque también es común agregarle cacao o coco. En este trabajo se evaluaron cambios microbiológicos durante la fermentación natural a temperatura ambiental, de tres tipos de pozol: blanco, con cacao y coco. La concentración de bacterias coliformes disminuyó a partir del tercer día de fermentación y a los 12 días se obtuvo una concentración de 2,20 log UFC/g. En las bacterias lácticas se observó el mayor crecimiento; ellas alcanzaron una concentración de 8,00 log UFC/g a los 3 días de fermentación que se mantuvo durante los 9 días siguientes. Se realizaron pruebas de nivel de agrado y preferencia con 31 jueces consumidores. La adición de ingredientes como el cacao o el coco no mejoraron el nivel de agrado entre los consumidores evaluados (p > 0,05. El pozol blanco y fresco fue el preferido (32 %. En el futuro, estos resultados pueden ser utilizados para clasificar al pozol como una bebida funcional, debido a la presencia de bacterias lácticas en concentración similar a la encontrada en el yogur.

  15. Evaluación microbiológica y sensorial de fermentados de pozol blanco, con cacao (Theobroma cacao y coco (Cocos nucifera

    Directory of Open Access Journals (Sweden)

    Román Jiménez Vera

    2010-06-01

    Full Text Available El pozol es una bebida de maíz que se consume en el sureste de México y en algunos países de Centroamérica. Se puede consumir recién elaborado o fermentado. Tradicionalmente se consume solo (pozol blanco, aunque también es común agregarle cacao o coco. En este trabajo se evaluaron cambios microbiológicos durante la fermentación natural a temperatura ambiental, de tres tipos de pozol: blanco, con cacao y coco. La concentración de bacterias coliformes disminuyó a partir del tercer día de fermentación y a los 12 días se obtuvo una concentración de 2,20 log UFC/g. En las bacterias lácticas se observó el mayor crecimiento; ellas alcanzaron una concentración de 8,00 log UFC/g a los 3 días de fermentación que se mantuvo durante los 9 días siguientes. Se realizaron pruebas de nivel de agrado y preferencia con 31 jueces consumidores. La adición de ingredientes como el cacao o el coco no mejoraron el nivel de agrado entre los consumidores evaluados (p > 0,05. El pozol blanco y fresco fue el preferido (32 %. En el futuro, estos resultados pueden ser utilizados para clasificar al pozol como una bebida funcional, debido a la presencia de bacterias lácticas en concentración similar a la encontrada en el yogur.

  16. Cálculo del costo de la prima de un seguro contra caída del precio de maíz blanco: Caso Sinaloa.

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    Rivera Silva, Ana Laura

    2010-01-01

    El presente trabajo compara el costo total de una póliza de seguros para la caída de precios del maíz blanco de Sinaloa contra el costo de la cobertura simple ofrecida por ASERCA. El comportamiento sistemático de los precios fue modelado con un modelo autorregresivo, mientras que la parte aleatoria fue manejada por un ajuste de una distribución de Laplace a los residuales. Los resultados muestran que la prima del seguro por tonelada es al menos tan buena como la prima para ofrecida para...

  17. Proyecto de una bodega para la elaboración de vino blanco acogido a la Denominación de Origen Rueda, en Medina del Campo, Valladolid.

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    Rallo Valluerca, María

    2015-01-01

    El objeto del presente proyecto es definir el proceso productivo y las instalaciones que albergarán las obras e instalaciones necesarias para el desarrollo de las actividades de elaboración, almacenamiento, crianza y comercialización de vinos blancos acogidos a la Denominación de Origen Rueda. La bodega se ubicará en el municipio de Medina del Campo ( Valladolid), sobre unas parcelas propiedad del promotor. La uva que se transformará procederá de viñedos, también propiedad del promotor. Se t...

  18. Plan de negocios para la comercialización de insecticidas basados en profenofos para el control de gusano blanco en el cultivo de papa

    OpenAIRE

    Vinueza Vinueza, Vanessa Viviana

    2009-01-01

    129 hojas : ilustraciones, 29 x 21 cm + CD-ROM 2853 El Plan de Negocios se enfoca en la comercialización de un insecticida nuevo en el mercado, dirigido al segmento de agricultores cultivadores de papa afectados por la plaga del gusano blanco (Premnotrypes vorax). El proyecto se encuentra estructurado en seis capítulos. El primero describe el negocio de agroquímicos, analiza a la empresa comercializadora, al producto y cuantifica el objetivo del plan con un incremento en la participación d...

  19. EL PELÍCANO BLANCO AMERICANO (PELECANUS ERYTHRORHYNCHUS EN COLOMBIA, CON COMENTARIOS SOBRE LOS EFECTOS DE LOS HURACANES EN EL CARIBE

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    ESTELA FELIPE A.

    2005-12-01

    Full Text Available Presentamos un registro fotográfico del Pelicano Blanco Americano (Pelecanus erythrorhynchus en un manglar de la isla de San Andrés, en el Caribe colombiano, con lo cual se confirma la presencia de esta especie en Colombia. Adicionalmente discutimos el posible efecto de los huracanes del Caribe en la dispersión de esta especie y los efectos que estas catástrofes naturales pueden tener en la conservación de la avifauna del Archipiélago de San Andrés y Providencia.

  20. EL PELÍCANO BLANCO AMERICANO (PELECANUS ERYTHRORHYNCHUS) EN COLOMBIA, CON COMENTARIOS SOBRE LOS EFECTOS DE LOS HURACANES EN EL CARIBE

    OpenAIRE

    ESTELA FELIPE A.; SILVA JOHN DOUGLAS; CASTILLO LUIS FERNANDO

    2005-01-01

    Presentamos un registro fotográfico del Pelicano Blanco Americano (Pelecanus erythrorhynchus) en un manglar de la isla de San Andrés, en el Caribe colombiano, con lo cual se confirma la presencia de esta especie en Colombia. Adicionalmente discutimos el posible efecto de los huracanes del Caribe en la dispersión de esta especie y los efectos que estas catástrofes naturales pueden tener en la conservación de la avifauna del Archipiélago de San Andrés y Providencia.

  1. Biorremediación de los efluentes de cultivo del camarón Litopenaeus vannamei (Boone, 1931 utilizando tapetes microbianos en un sistema de recirculación Bioremediacion of effluents ones of the culture of Litopenaeus vannamei (Boone, 1931 using microbial mats in a recirculating system

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    Carlos Lezama-Cervantes

    2010-01-01

    Full Text Available Se desarrolló un sistema de recirculación (SR integrando el cultivo del camarón con tapetes microbianos buscando mitigar los impactos ambientales de los subproductos del cultivo del camarón y avanzar hacia la biorremediación del agua de cultivo. Los consorcios microbianos obtenidos de ambientes naturales (TaM estuvieron constituidos por bacterias (55,6%, cianófitas (18,4%, diatomeas (9%, nemátodos (5,6% y clorófitas (1,4%, entre los grupos taxonómicos principales. La remoción de nutrientes y sólidos se evaluó en un SR (2 ciclos/hora conteniendo (n = 3 60 y 120 ind m-2 de Litopenaeus vannamei. Los resultados demostraron que los TaM redujeron los niveles de nitrógeno amoniacal por encima del 71% diariamente; la demanda bioquímica de oxígeno (DBO5 se redujo más de 68% y los sólidos suspendidos (SST hasta en 62% al compararse con los sistemas control (p 0,05. El agua bio-remediada tiene un efecto positivo en el cultivo de Litopenaeus vannamei, y promueve el crecimiento y sobrevivencia en presencia de TaM en el SR.We developed a recirculating system integrating shrimp culture with microbial mats aiming to mitigate the environmental impacts of by-products from shrimp culture and to advance toward the bioremediation of the shrimp culture water. The microbial consortia collected from natural environments (TaM was constituted by bacteria (55.6%, Cyanophyte (18.4%, diatoms (9%, nematods (5.6% and Chlorophyte algae (1.4% among the major taxonomic groups. The removal of nutrients and solids by these mats was assessed in a recirculating system (2 cycles/hour (n = 3 containing 60 and 120 ind m-2 of Litopenaeus vannamei. Results showed that the microbial mat reduces daily up to 71% of ammonia nitrogen, decreases around 68% of the biochemical oxygen demand (DBO5 and up to 62% of suspended solids (SST when comparing to the control systems (p 0.05. The Bioremediated culture water had a positive effect on the culture of Litopenaeus vannamei, and

  2. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem = Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies

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    Paulo de Paula Mendes

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (pLitopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, withdifferent concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p<0.05 the zootechnic parameters of the species in a significant way.

  3. Caracterización cariotípica en mitosis y meiosis del robalo blanco Centropomus undecimalis (Pisces: Centropomidae

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    Lenin Arias-Rodriguez

    2011-06-01

    Full Text Available El robalo blanco Centropomus undecimalis, vive en hábitats marinos, salobres y dulceacuícolas en el océano Atlántico occidental, incluyendo el golfo de México. La especie, es económicamente importante en varias localidades, no obstante los estudios sobre su biología y genética son hasta el momento pocos. El presente estudio tiene como propósito, la caracterización citogenética de especímenes recolectados en el municipio de Paraíso, Tabasco, México. Cinco hembras y ocho machos fueron procesados por técnicas citológicas convencionales para la obtención de preparaciones cromosómicas de buena calidad para elaborar el cariotipo. Los resultados del análisis del tejido del riñón, mostraron que 85.1% de 288 mitosis tienen 2n=48 cromosomas y 52.8% de 104 meiosis exhiben el número haploide de 1n=24. El cariotipo diploide mostro 48 cromosomas monorrámeos de tipo telocéntrico (T. No se observó heteromorfismo cromosómico entre hembras y machos. El cariotipo diploide fue similar a los observados en la mayoría de peces marinos.Karyotypic characterization in mitosis and meiosis of the common snook Centropomus undecimalis (Pisces: Centropomidae. The common snook Centropomus undecimalis inhabits marine, brackish and freshwater habitats in the Western Central Atlantic Ocean, including the Gulf of Mexico. Common snook is an economically important fish in many localities, nevertheless the number of studies on its biology and genetics are still few. The present study attempts to establish the cytogenetic profiles of the specimens collected in Paraiso Municipality Tabasco, Mexico. Tissue of five females and eight male organisms were processed by conventional cytological techniques to obtain chromosome slides of high quality in order to assemble the karyotype. The results from the kidney tissue analysis showed that 85.1% of 288 mitosis had a 2n=48 chromosomes, and 52.8% of 104 meiosis exhibited the haploid number 1n=24. The diploid karyotype

  4. Frantz Fanon y la Enajenación del Negro y del Blanco en el Sistema Colonial

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    Ana Catarina Zema de Resende

    2016-11-01

    Full Text Available Resumen A partir de la lectura de Piel negra, máscaras blancas, propongo en este artículo reflexionar sobre las ideas de Frantz Fanon a cerca de la enajenación del negro y del blanco en el sistema colonial. El objetivo es recuperar algunos de los principales aportes de Fanon para la comprensión del racismo colonial desde su teoría de la enajenación. Fanon presenta la enajenación como un paso previo a la esclavitud y al colonialismo, necesario para el mantenimiento de la explotación económica y analiza las conductas identitarias de "vergüenza de sí" como el resultado de la dominación colonial. Él describe con precisión el impacto del racismo y del colonialismo y sus efectos destructivos mostrando cómo los mecanismos de enajenación determinan la relación entre el blanco y el negro y cómo se reproducen las jerarquías que rigen estas relaciones. Retomar el pensamiento de Fanon y reconocer la pertinencia y actualidad de sus contribuciones es esencial para poder reubicar la lucha contra todas las formas de dominación en la continuación de la lucha contra el colonialismo en una época en que la identidad racial y el racismo han más que probado su capacidad de persistir en el tiempo y el espacio. Palabras clave: Frantz Fanon; Enajenación; Racismo; Sistema Colonial. Frantz Fanon and the Alienation of Black and White in the Colonial System Abstract From the reading of Black Skin, White Masks, in this article I propose to reflect on Frantz Fanon's ideas about alienation of black and white in the colonial system. The goal is to recover some of his most important contributions to the understanding of colonial racism from his theory of alienation. Fanon presents alienation as a prior step to slavery and colonialism, necessary for the maintenance of economic exploitation and analyzes the identity conducts of self shame as a result of colonial domination. Fanon accurately describes the impact of racism and colonialism and its destructive

  5. Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei.

    Science.gov (United States)

    Chimetto, Luciane A; Cleenwerck, Ilse; Alves, Nelson; Silva, Bruno Sergio; Brocchi, Marcelo; Willems, Anne; De Vos, Paul; Thompson, Fabiano L

    2011-02-01

    Eight Vibrio isolates originating from the marine corals Mussismilia hispida and Phyllogorgia dilatata and the zoanthids Palythoa caribaeorum and Palythoa variabilis in Brazil and the Pacific white shrimp (Litopenaeus vannamei) in Ecuador were studied by means of a polyphasic approach. The novel isolates formed a tight monophyletic group in the genus Vibrio and were closely related to species of the Vibrio harveyi group, to which they showed more than 99 % 16S rRNA gene sequence similarity. Analysis based on concatenated sequences of the following seven genes, 16S rRNA, gyrB, recA, rpoA, topA, pyrH and mreB (5633 bp in length), showed clear separation between the isolates and species of the V. harveyi group. Amplified fragment length polymorphism (AFLP) analysis, performed previously, revealed that a representative isolate of this group, LMG 20370, was clearly separate from known Vibrio species (it belonged to the so-called AFLP cluster A31). DNA-DNA hybridization (DDH) experiments with representative isolates and type strains of the V. harveyi species group revealed high DDH between the novel isolates (more than 74 %) and less than 70 % DDH towards type strains of related Vibrio species, proving the novel species status of the isolates. Phenotypically, the novel species belongs to the arginine dihydrolase (A)-negative, lysine decarboxylase (L)-positive and ornithine decarboxylase (O)-positive (A-/L+/O+) cluster reported previously. Most species of the V. harveyi group (i.e. Vibrio rotiferianus, V. harveyi, V. parahaemolyticus and V. alginolyticus) also belong to this A-/L+/O+ cluster. However, several phenotypic features can be used for the identification of the novel species. In contrast to its closest phylogenetic neighbours, the novel species exhibits esterase (C4) and N-acetyl-β-glucosaminidase activities, but it does not produce acetoin, does not use citrate, α-ketoglutaric acid or propionic acid and does not ferment melibiose. The novel species can

  6. Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) co-infection on survival of penaeid shrimp Litopenaeus vannamei

    Science.gov (United States)

    Zhang, Xiaojing; Song, Xiaoling; Huang, Jie

    2016-11-01

    White spot syndrome virus (WSSV) is an important viral pathogen that infects farmed penaeid shrimp, and the threat of Vibrio parahaemolyticus infection to shrimp farming has become increasingly severe. Viral and bacterial cross or superimposed infections may induce higher shrimp mortality. We used a feeding method to infect Litopenaeus vannamei with WSSV and then injected a low dose of V. parahaemolyticus (WSSV+Vp), or we first infected L. vannamei with a low-dose injection of V. parahaemolyticus and then fed the shrimp WSSV to achieve viral infection (Vp+WSSV). The eff ect of V. parahaemolyticus and WSSV co-infection on survival of L. vannamei was evaluated by comparing cumulative mortality rates between experimental and control groups. We also spread L. vannamei hemolymph on thiosulfate citrate bile salt sucrose agar plates to determine the number of Vibrio, and the WSSV copy number in L. vannamei gills was determined using an absolute quantitative polymerase chain reaction (PCR) method. LvMyD88 and Lvakt gene expression levels were detected in gills of L. vannamei by real-time PCR to determine the cause of the diff erent mortality rates. Our results show that (1) the cumulative mortality rate of L. vannamei in the WSSV+Vp group reached 100% on day 10 after WSSV infection, whereas the cumulative mortality rate of L. vannamei in the Vp+WSSV group and the WSSV-alone control group approached 100% on days 11 and 13 of infection; (2) the number of Vibrio in the L. vannamei group infected with V. parahaemolyticus alone declined gradually, whereas the other groups showed significant increases in the numbers of Vibrio ( P<0.05); (3) the WSSV copy numbers in the gills of the WSSV+Vp, Vp+WSSV, and the WSSV-alone groups increased from 105 to 107 /mg tissue 72, 96, and 144 h after infection, respectively. These results suggest that V. parahaemolyticus infection accelerated proliferation of WSSV in L. vannamei and vice versa. The combined accelerated proliferation of both V

  7. Apparent carbohydrate and lipid digestibility of feeds for whiteleg shrimp, Litopenaeus vannamei (Decapoda: Penaeidae, cultivated at different salinities

    Directory of Open Access Journals (Sweden)

    Milena Gucic

    2013-09-01

    Full Text Available Whiteleg shrimp, Litopenaeus vannamei is one of the most commercially farmed species worldwide because of its fast growth, good survival rate at high farming densities, and osmoregulatory capacity, which makes it an excellent candidate for cultures at different salinities. The knowledge of shrimp nutritional requirements is critical in the formulation of diets to allow optimal growth at different environmental conditions and development stages. The effect of salinity on apparent digestibility of shrimp feed is not well known, and this information is required in shrimp diet formulation. For this purpose, the apparent digestibility coefficients of carbohydrates (ACD and lipids (ALD were determined for juvenile whiteleg shrimps under controlled culture conditions. We evaluated the apparent digestibility of six commercial (D1:37CP, D2:38CP, D3:39CP, D4:34CP, D5:35CP, and D6:37CP and two experimental (E1:33CP and E2:33CP diets for juvenile whiteleg shrimp cultivated at three salinities (5, 35 and 50psu in 60L aquariums. ACD and ALD were determined in vivo using chromic oxide as an inert marker. Our results showed that ALD in most cases was over 80%, independent of salinity, except the E1:33CPdiet which had 74.0% at 50psu. Diet D3:39CP showed the highest ALD coefficient (90.1 and 90.6% at 5 and 35psu, respectively. For ACD, differences were detected between commercial and experimental diets at every salinity level, although salinity effect on ACD was not significant. Diet D4:34CP had the highest coefficient (92.4% at 5psu, and E2:33CP at 35 and 50psu (97.3 and 94.7%. This study demonstrated that there is no significant effect of saline variations on carbohydrate and lipid digestibility by juvenile whiteleg shrimp, under the experimental conditions. Rev. Biol. Trop. 61 (3: 1201-1213. Epub 2013 September 01.

  8. Citrus reticulata Blanco.

    African Journals Online (AJOL)

    RACHEL

    In Sylhet region of Bangladesh it is locally known as Zara lemon and available in the local. *Corresponding ... While the lemon or orange are peeled to consume their pulpy and juicy segments, the citron's pulp is ... Then washing of the explant took place with distilled water for. 3 to 4 times. Before inoculation soaking of the ...

  9. mandarin (Citrus reticulata Blanco)

    African Journals Online (AJOL)

    ARL4

    Random amplified polymorphic DNA (RAPD) analysis of 'Dangshansu Pear' sports '97-. 05-9'. Chin. Agric. Sci. Bull. 26:48-53. Gong GZ, Hong QB, Peng ZC, Jiang D, Xiang SQ (2008). Genetic diversity of poncirus and its phylogenetic relationships with relatives as revealed by nuclear and chloroplast SSR. Acta Hortic. Sin.

  10. Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931 (Decapoda, Penaeidae and its implication for the gene pool conservation

    Directory of Open Access Journals (Sweden)

    PD. Freitas

    Full Text Available Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

  11. Does damming of the Colorado River affect the nursery area of blue shrimp Litopenaeus stylirostris (Decapoda: Penaeidae in the Upper Gulf of California?

    Directory of Open Access Journals (Sweden)

    Eugenio Alberto Aragón-Noriega

    2000-12-01

    Full Text Available After damming the Colorado River the freshwater flow was reduced to 1 % of its virgin flow to the Upper Gulf of California (UGC. The ecological effects need to be properly documented. The UGC is the nursery area for Litopenaeus stylirostris, the most profitable fishery in the zone. In order to know the relative abundance of L. stylirostris postlarval stage we conducted a sampled survey every 14 days in 1993, 1994 and 1997, plus an intensive sampling during a complete tide cycle in July 1995 and 1996. We did 10 min trawls each hour during the flood tide. Relative abundance of postlarvae was higher (pEl represamiento del Río Colorado ha ocasionado que el flujo de agua dulce sobre el Alto Golfo de California (AGC se haya reducido hasta el 1 % del flujo original. Se ha documentado el efecto de la reducción de agua dulce sobre las condiciones hidrográficas del AGC, pero las repercusiones ecológicas no se han descrito apropiadamente. El AGC ha sido área de crianza para especies comerciales como el camarón Litopenaeus stylirostris. Se hicieron recolectas de postlarvas de L. stylirostris en el AGC durante cinco años consecutivos. Los muestreos fueron catorcenalmente en los años de 1993, 1994 y 1997 y se realizó una recolecta diaria durante 15 días consecutivos en los años 1995 y 1996. Para ello se arrastró una red de plancton de 505 µ durante 10 min cada hora durante el flujo de marea. La abundancia relativa de las postlarvas de camarón en esta zona viaria considerablemente en años cuando el flujo de agua dulce incrementa. La abundancia es mayor hasta en un 200 % (p < 0.05 cuando existe descarga de agua dulce al AGC.

  12. Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes

    OpenAIRE

    Cardona, Emilie; Lorgeoux, Benedicte; Geffroy, Claire; Richard, Pierre; Saulnier, Denis; Gueguen, Yannick; Guillou, Gael; Chim, Liet

    2015-01-01

    The aim of this study was to assess the relative contribution of natural productivity and compound food to the growth of the juvenile blue shrimp Litopenaeus stylirostris reared in a biofloc system. Two experiments were carried out based on the same protocol with three treatments: clear water with experimental diet (CW), biofloc with experimental diet (BF) and biofloc unfed (BU). Shrimp survival was significantly higher in biofloc rearing than in CW rearing. The contribution of the biofloc to...

  13. SEBARAN INFEKSI TAURA SYNDROME, INFECTIOUS MYONECROSIS, DAN Penaeus vannamei NERVOUS VIRUS (TSV, IMNV, DAN PvNV) PADA BUDIDAYA UDANG Litopenaeus vannamei DI JAWA BARAT, JAWA TIMUR, DAN BALI

    OpenAIRE

    Isti Koesharyani; Lila Gardenia; Tatik Mufidah

    2015-01-01

    Pada budidaya udang introduksi Litopenaeus vannamei, virus merupakan penyakit yang memberi dampak cukup merugikan dan menimbulkan kematian massal budidaya udang vaname. Tujuan penelitian ini adalah untuk mengetahui sebaran adanya infeksi virus di beberapa daerah budidaya udang L. vannamei, di Jawa Timur (Bangil, Banyuwangi, Situbondo), Bali, dan Jawa Barat (Karawang dan Mauk-Tangerang). Jenis virus yang dianalisis adalah Taura Syndrome (TSV), Infectious Myonecrosis (IMNV), dan Penaeus vanname...

  14. Informe de las condiciones oceanográficas superficiales entre Chicama y Cabo Blanco. Crucero BAP Ocoña 9510: Del 16 al 23 de Octubre de 1995.

    OpenAIRE

    Pizarro, Luis; García Díaz, Walter

    1996-01-01

    Presenta un monitoreo oceanográfico pesquero del 16 al 24 de Octubre de 1995 abarcando las zonas entre Puerto Salaverry y el norte de Cabo Blanco, a fin de analizar la variación horizontal de la temperatura y salinidad del mar.

  15. Effect synergistic of the radiation and of the refrigeration in the conservation of the white shrimp (Litopenaeus vannamei);Efeito sinergistico da radiacao gama e de refrigeracao na conservacao do camarao-branco-do-pacifico (Litopenaeus vannamei)

    Energy Technology Data Exchange (ETDEWEB)

    Guimaraes-Lopes, Tatiana Giselle

    2006-07-01

    Taking into account shrimp susceptibility to autolise and bacterial decomposition, this study aimed to evaluate the different gamma irradiation doses influence over meat quality related to microbiological, physical-chemical, nutritional and sensorial aspects, during cold storage of the white shrimp (Litopenaeus vannamei). Samples were taken from fresh shrimps without cephalothoraxes, settled into polystyrene trays, packed with flexible plastic film and submitted to different doses of gamma irradiation (1.0 and 3.5 kGy). Samples were kept under refrigeration (5 deg C) and analyzed at the 1st, 7th, 14th and 21st day of storage. Microbiological analyzes were realized (Coagulase-positive Staphylococcus, Salmonella ssp, coliforms, psychotropic and mesophilic population), and physical-chemical and tests (Total volatile bases (TVNB), pH, non proteic nitrogen (NNP), TBARS analysis - reactive substances to thiobarbituric acid (TBA), trimetil amine (TMA) cholesterol tenor and proximate composition). Instrumental color test were realized and hedonic test was taken to determine cooked products sensorial quality during storing period. It was noticed that the irradiation process reduced bacterial population and during storage all the irradiated samples were under the patterns established by the Brazilian legislation (Brazil, 2005a). Non-irradiated samples were inappropriate for consumption after one week of storage, since they were over Staphylococcus established limits. The pH values ranged from 6.05 to 6.39; 19.47 to 80.84 mg.100g-1 for TVNB; 2.61 to 12.85 mg.100g-1 for TMA-N; 0.22 a 1.20 mg of malonaldeide.1000g{sup -1} for TBA and 53.09 to 134.81 mg.100g-1 for cholesterol concentrations. Irradiated samples kept more stability during storage period when compared to non-irradiated samples, regarding to pH, TVNB and TMA levels. Samples irradiation resulted into cholesterol and TBA values reduction at different evaluated moments. No significant differences (p <= 0.05) were found

  16. El día más blanco o el país de la memoria de Raúl Zurita

    Directory of Open Access Journals (Sweden)

    María Luisa Fischer

    2014-06-01

    Full Text Available Estudio la adscripción genérica de El día más blanco del poeta chileno Raúl Zurita, así como la significación de los epígrafes, otra forma de enmarcación del volumen. Entre la novela y la autobiografía, el libro se propone cubrir la grieta que existe entre la memoria personal y la memoria traumática del país. Describo la especial calidad visual de las imágenes del texto y su relación con las dificultades de construir una versión de la historia subjetiva y personal que se aúne a la historia del país, cuando ambas están quebradas.

  17. DESARROLLO TECNOLÓGICO PARA ELABORAR VINO BLANCO COMÚN EN MISIONES, CON EVALUACIÓN ECONÓMICA A ESCALA INDUSTRIAL

    Directory of Open Access Journals (Sweden)

    Miño Valdés , Juan Esteban

    2013-01-01

    Full Text Available El objetivo de este trabajo fue desarrollar una tecnología sustentable a escala industrial para elaborar vino blanco común, con uvas no viníferas cultivadas en Misiones. Este proyecto tecnológico se inició a escala laboratorio, continuó en planta piloto y proyectó a escala industrial. Se consideró como unidad productiva a 12 familias rurales con 27 ha de viñedo cada una. Las 8 etapas seguidas con metodología inductiva y deductiva fueron: La elaboración de vino blanco seco a escala laboratorio. La evaluación de las variables del proceso en las vinificaciones. El modelo matemático de la fermentación alcohólica en condiciones enológicas. La valoración de la aptitud de los vinos para el consumo humano. El establecimiento de un procedimiento tecnológico para la vinificación en planta piloto. La evaluación en planta piloto del procedimiento tecnológico establecido. El cálculo y la selección del equipamiento industrial. La estimación de los costos y la rentabilidad del proceso tecnológico industrial. Se alcanzó una tecnología para una capacidad de producción de 5.834 L (litros día-1, con indicadores económicos dinámicos cuyos valores fueron: valor actualizado neto de 6.602.666 U$D, una taza interna de retorno del 60 % para un período de recuperación de inversión a valor actualizado neto de 3 años.

  18. EFECTO DE LA RESTRICCIÓN ALIMENTICIA Y LA REALIMENTACIÓN SOBRE LA COMPOSICIÓN DEL MÚSCULO BLANCO DE Piaractus brachypomus

    Directory of Open Access Journals (Sweden)

    F. Y. Riaño

    2011-01-01

    Full Text Available Con el propósito de evaluar el efecto de la restricción alimenticia y la realimentación sobre la composición del músculo blanco de cachama blanca, se aplicaron dos protocolos de restricción alimenticia durante 84 días, cada uno dividido en un periodo de restricción alimenticia y un periodo de realimentación. En el primer protocolo, se aplicó una restricción alimenticia moderada (33,3%, y en el segundo, una restricción severa (50%; ambos grupos fueron comparados con un grupo control que recibió una ración alimenticia igual a la suministrada bajo condiciones de un cultivo comercial. Al finalizar el ensayo, ninguno de los tiempos de restricción alimenticia tuvo efectos significativos (p > 0,05 sobre el porcentaje de proteína del filete. Sin embargo, se observaron efectos significativos (p < 0,05 sobre los porcentajes de lípidos, cenizas y energía. Con respecto al porcentaje de cenizas musculares, se observó que la restricción alimenticia tendió a aumentar su valor, mientras que para el porcentaje de lípidos y los niveles de energía, se encontró el efecto contrario. No obstante, cuando los individuos finalizaron el periodo de realimentación, se observó un restablecimiento en los niveles de nutrientes comparados con los individuos no restringidos. En cuanto al perfil de ácidos grasos, el grupo control mostró con diferencias significativas (p < 0,05 mayor porcentaje de omega-3 (n-3. Estos resultados permiten concluir que los protocolos de alimentación aplicados estimularon, en Piaractus brachypomus, movilización de nutrientes musculares y su posterior restablecimiento, sin afectar la integridad del músculo blanco.

  19. Turbidite pathways in Cascadia Basin and Tufts abyssal plain, Part A, Astoria Channel, Blanco Valley, and Gorda Basin

    Science.gov (United States)

    Wolf, Stephen C.; Hamer, Michael R.

    1999-01-01

    This open-file report was prepared in support of the USGS Earthquake Hazards of Cascadia Project. The primary objective of this phase of the project is to determine recurrence intervals of turbidites in Cascadia basin-floor channel systems and evaluate implications of this event record for the paleoseismic history of the Cascadia subduction zone. The purpose of this study is to determine whether the canyon/channel systems themselves are blocked or deformed in such a way that the downstream turbidite stratigraphy might be biased. To accomplish this investigation approximately 7500 kilometers of pre-existing 3.5 KHz seismic data were evaluated to determine the direction and extent of the Astoria Channel/pathway system, which originates at the base of the Astoria Fan. Additionally, distribution and thickness of turbidite sediment sequences were determined along each identified pathway. Bathymetery and distance were used to determine gradients along the main pathway axis and for each of the secondary pathways that feed into it. Channel pathways were identified on the basis of channel phyisiography, where visible at the seafloor, subbottom channel configuration, and acoustic packets of sediments that might represent turbidite deposits. A principal result of this study is that the Astoria Channel/pathway extends continuously from the base of the Astoria Fan southward along the base of the continental slope through the Blanco Valley, then heads southwestward through the Gorda Basin and into the region of the Escanaba Trough. Additionally it was determined that the Astoria Channel is filled and basically buried for it's full length south of 44 degrees latitude. The 44 North Slump, as defined by Goldfinger (1999, see Map 3 ref.), may have been instrumental in blocking the pathway and thus contributed to the filling of the channel/pathway. Sheets 1 and 2 show the Astoria and secondary turbidite pathways highlighted in blue. Ship survey tracklines are shown for the area

  20. Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471 Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil - doi: 10.4025/actascibiolsci.v32i3.5471

    Directory of Open Access Journals (Sweden)

    Adolfo Jatoba

    2010-09-01

    Full Text Available This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p V. alginolyticus isolated from larvae and juvenile reared marine shrimp.This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by

  1. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from RIO BLANCO in the English Channel, Mediterranean Sea and others from 2009-12-13 to 2010-12-14 (NODC Accession 0117291)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0117291 includes Surface underway data collected from RIO BLANCO in the English Channel, Mediterranean Sea, North Atlantic Ocean, South Atlantic Ocean...

  2. A propósito de la semiótica y las ciencias humanas. (Comentario al artículo de Desiderio Blanco

    Directory of Open Access Journals (Sweden)

    Óscar Quezada Machiavello

    2011-05-01

    Full Text Available Desiderio Blanco ha presentado una ponencia al Seminario-Taller deInvestigación «Fernando Tola Mendoza» organizado por la Facultad de Letras yCiencias Humanas de la Universidad Nacional Mayor de San Marcos tituladaSemiótica y Ciencias Humanas en la que reflexiona en torno a esa relación en laperspectiva de la interdisciplinariedad. En un primer momento, da cuenta de laconfluencia de disciplinas en la que nace la semiótica moderna y explicasomeramente las dos direcciones que sigue (semiología, que se ocupa del signo; ysemiótica, que se ocupa de la significación en discurso. Asume luego la opción dela semiótica tal como es caracterizada a partir de la obra de A. J. Greimas. El métodosemiótico consiste, entonces, en observar de dos modos cómo se genera lasignificación en el discurso: sea que se trate de la significación en el discurso terminadoo, más bien, en el discurso en acto. El primer modo se vincula con la etapaestructuralista y generativa de la semiótica (que se resume del modo más económicoen el modelo del recorrido generativo. El segundo modo, sin abandonar el anterior,quiere rendir cuenta del discurso viviente, y no solamente de sus estructurasformales desprendidas de su enunciación. Desde ese momento, a los paradigmasestructural y generativo se suma el paradigma tensivo y sensible: en consecuencia,prevalecerá el punto de vista de la praxis enunciativa en cuanto que «aventaja», sinreemplazarlo, al del recorrido generativo.En un segundo momento, Blanco identifica de modo muy general los aportesde la lingüística, de las ciencias cognitivas, de las corrientes formalistas, de lafenomenología y de la filosofía. Para terminar de caracterizar la problemática de lainterdisciplinariedad, profundiza el concepto-modelo de praxis enunciativa quecondensa de modo más económico la elaboración teórica actual.Ante el pedido que se me ha hecho de comentar esa ponencia, he optado ya nopor pensar las ciencias humanas desde el

  3. Algo más sobre la infundada atribución a Blanco White de la novela «Vargas», de Alexander Dallas, con unas páginas inéditas de Vicente Llorens

    Directory of Open Access Journals (Sweden)

    Fernando Durán López

    2016-07-01

    Full Text Available Este breve artículo aporta algunas evidencias adicionales a las que ya presenté en Cuadernos de Ilustración y Romanticismo, nº 19 (2013, sobre la autoría de Vargas, a tale of Spain, novela escrita por el escritor inglés Alexander Dallas, pero habitualmente atribuida a José María Blanco White. Por una parte, se informa de la localización en la Biblioteca Nacional de Madrid del manuscrito original de Vargas; por otra, se publica un texto inédito de Vicente Llorens con sus argumentos para defender que la obra no fue escrita por Blanco White.

  4. Rufino Blanco Sánchez y las fuentes bibliográficas de la educación física y el deorte en España

    OpenAIRE

    Torrebadella Flix, Xavier

    2014-01-01

    Rufino Blanco y Sánchez materializó una de las mayores contribuciones pedagógicas, académicas y científicas de la educación física contemporánea. Esto sucedió en el primer tercio del siglo pasado, cuando la educación física en España trataba de legitimar su espacio institucional en el sistema educativo y el deporte moderno estimulaba la mayor apuesta asociativa de la historia. Entre la abundante obra pedagógica y bibliográfica de Blanco, sobresale la Bibliografía General de la Educación Físic...

  5. Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

    Directory of Open Access Journals (Sweden)

    Rosalia Contreras

    2011-06-01

    Full Text Available Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs; Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM—carbohydrates (CHO basal feed. Treatment 2 (B + Bm; Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B; SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C; fishmeal commercial feed (FM was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs; additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B presented smaller growth and lower feed conversion ratio (FCR. Shrimps fed with the fishmeal commercial feed (C presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

  6. The effects of feeding commercial feed formulated for semi-intensive systems on Litopenaeus vannamei production and its profitability in a hyper-intensive biofloc-dominated system

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    André Braga

    2016-05-01

    Full Text Available The present study evaluated the production of Litopenaeus vannamei in a high density biofloc-dominated system using two commercial feeds; a less expensive feed (US$0.99 kg−1 formulated for semi-intensive systems and the more expensive (US$1.75 kg−1 which was designed for hyper-intensive systems. A 67-days study was conducted in six 40 m3 lined with Ethylene Propylene Diene Monomer membrane raceways (RWs filled with mixture of seawater (22 m3, and biofloc-rich water (18 m3. Each RW was stocked (500 shrimp m−3 using juveniles (2.66 g produced from Taura-Resistant and Fast-Growth breeding lines. The study was composed of two feed treatments with three replicates each; the cheaper feed (SI-35 contained 35% crude protein (CP, 7% lipid and 4% fiber while the more expensive one (HI-35 had the same levels of CP and lipid but only 2% fiber. The SI-35 treatment required more solids removal, oxygen, and bicarbonate supplementation than the HI-35 treatment. Weekly growth, total biomass, yield were significantly lower in the SI-35 treatment, whereas feed conversion ratio was higher. The economic analysis indicates that both feeds would be commercially viable, nevertheless, the less-expensive feed financially underperformed the other.

  7. Functional feed assessment on Litopenaeus vannamei using 100% fish meal replacement by soybean meal, high levels of complex carbohydrates and Bacillus probiotic strains.

    Science.gov (United States)

    Olmos, Jorge; Ochoa, Leonel; Paniagua-Michel, Jesus; Contreras, Rosalia

    2011-01-01

    Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids) and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs); Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM)-carbohydrates (CHO) basal feed. Treatment 2 (B + Bm); Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B); SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C); fishmeal commercial feed (FM) was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone) Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs); additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B) presented smaller growth and lower feed conversion ratio (FCR). Shrimps fed with the fishmeal commercial feed (C) presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

  8. Combined use of DGT and transplanted shrimp (Litopenaeus vannamei) to assess the bioavailable metals of complex contamination: implications for implementing bioavailability-based water quality criteria.

    Science.gov (United States)

    Wang, Zaosheng; Zhao, Peihong; Yan, Changzhou; Chris, Vulpe D; Yan, Yijun; Chi, Qiaoqiao

    2014-03-01

    The diffusive gradients in thin films (DGT) were field deployed alongside the shrimp Litopenaeus vannamei at seven sites with different levels of contamination to assess the potentially bioavailable and toxic fraction of metal contaminants. After 7 days of exposure, several antioxidant biomarkers were quantified in hepatopancreas of exposed shrimps, and tissue levels as well as the total, dissolved, and DGT-labile concentrations of metal contaminants were determined in the pooled site samples. The results showed that the caged shrimps had high tissue contaminant concentrations and significantly inhibited antioxidant responses at the more contaminated sites. DGT-labile metal concentrations provided better spatial resolution of differences in metal contamination when compared with traditional bottle sampling and transplanted shrimp. The total, dissolved, and DGT-labile metal fractions were used to evaluate the potential bioavailability of metal contaminants, comparing with metal accumulation and further linking to antioxidant biomarker responses in tissues of exposed shrimps. Regression analysis showed the significant correlations between DGT-Cu concentrations and tissue-Cu and activities of some biomarker responses in the shrimp hepatopancreas. This indicated that DGT-labile Cu concentrations provided the better prediction of produced biological effects and of the bioavailability than the total or dissolved concentrations. The study supports the use of methods combining transplanted organisms and passive sampling for assessing the chemical and ecotoxicological status of aqueous environments and demonstrates the capability of the DGT technique as a powerful tool for measuring the bioavailability-based water quality in variable coastal environments.

  9. Effect of replacing fish meal with extruded soybean meal on growth, feed utilization and apparent nutrient digestibility of juvenile white shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Yang, Qihui; Tan, Beiping; Dong, Xiaohui; Chi, Shuyan; Liu, Hongyu

    2015-10-01

    Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannamei. In the control diet (Diet 1), FM protein was replaced with increasing dietary levels of ESBM (4.28%, 8.40%, 12.62%, 16.82%, and 25.26%) at 10%, 20%, 30%, 40%, and 60% levels (Diets 2 to 6, respectively). An eight-week feeding trial was conducted on 720 juvenile shrimp (0.67 g ± 0.01 g mean initial weight), and nutrient digestibility of the six diets was determined. ESBM could replace 20% of FM without causing a significant reduction in growth of shrimp, but other dietary treatments strongly affected whole body composition. Crude protein content of the whole body fed Diet 6 was significantly lower than that fed Diet 2 ( P replacement level. This study indicates that 20% FM replacement with ESBM in the basic diet containing 40% protein and 30% FM is optimal for juvenile L. vannamei.

  10. Crystallographic studies evidencing the high energy tolerance to disrupting the interface disulfide bond of thioredoxin 1 from white leg shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Campos-Acevedo, Adam A; Rudiño-Piñera, Enrique

    2014-12-15

    Thioredoxin (Trx) is a small 12-kDa redox protein that catalyzes the reduction of disulfide bonds in proteins from different biological systems. A recent study of the crystal structure of white leg shrimp thioredoxin 1 from Litopenaeus vannamei (LvTrx) revealed a dimeric form of the protein mediated by a covalent link through a disulfide bond between Cys73 from each monomer. In the present study, X-ray-induced damage in the catalytic and the interface disulfide bond of LvTrx was studied at atomic resolution at different transmission energies of 8% and 27%, 12.8 keV at 100 K in the beamline I-24 at Diamond Light Source. We found that at an absorbed dose of 32 MGy, the X-ray induces the cleavage of the disulfide bond of each catalytic site; however, the interface disulfide bond was cleaved at an X-ray adsorbed dose of 85 MGy; despite being the most solvent-exposed disulfide bond in LvTrx (~50 Å2). This result clearly established that the interface disulfide bond is very stable and, therefore, less susceptible to being reduced by X-rays. In fact, these studies open the possibility of the existence in solution of a dimeric LvTrx.

  11. Crystallographic Studies Evidencing the High Energy Tolerance to Disrupting the Interface Disulfide Bond of Thioredoxin 1 from White Leg Shrimp Litopenaeus vannamei

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    Adam A. Campos-Acevedo

    2014-12-01

    Full Text Available Thioredoxin (Trx is a small 12-kDa redox protein that catalyzes the reduction of disulfide bonds in proteins from different biological systems. A recent study of the crystal structure of white leg shrimp thioredoxin 1 from Litopenaeus vannamei (LvTrx revealed a dimeric form of the protein mediated by a covalent link through a disulfide bond between Cys73 from each monomer. In the present study, X-ray-induced damage in the catalytic and the interface disulfide bond of LvTrx was studied at atomic resolution at different transmission energies of 8% and 27%, 12.8 keV at 100 K in the beamline I-24 at Diamond Light Source. We found that at an absorbed dose of 32 MGy, the X-ray induces the cleavage of the disulfide bond of each catalytic site; however, the interface disulfide bond was cleaved at an X-ray adsorbed dose of 85 MGy; despite being the most solvent-exposed disulfide bond in LvTrx (~50 Å2. This result clearly established that the interface disulfide bond is very stable and, therefore, less susceptible to being reduced by X-rays. In fact, these studies open the possibility of the existence in solution of a dimeric LvTrx.

  12. Dynamics of vitellogenin and vitellogenesis-inhibiting hormone levels in adult and subadult whiteleg shrimp, Litopenaeus vannamei: relation to molting and eyestalk ablation.

    Science.gov (United States)

    Kang, Bong Jung; Okutsu, Tomoyuki; Tsutsui, Naoaki; Shinji, Junpei; Bae, Sun-Hye; Wilder, Marcy N

    2014-01-01

    Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.

  13. Isolation and cDNA cloning of a novel red colour-related pigment-binding protein derived from the shell of the shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Pan, Chuang; Ishizaki, Shoichiro; Nagashima, Yuji; Gao, Jialong; Watabe, Shugo

    2018-02-15

    Pigment-binding proteins play important roles in crustacean shell colour change. In this study, a red colour-related pigment-binding protein, designated LvPBP75, was purified from the shell of Litopenaeus vannamei. HPLC and PAGE analysis showed that LvPBP75 was a homogeneous monomer with molecular mass of 75kDa. Peptide mass fingerprint analysis revealed that LvPBP75 belonged to hemocyanin, and the released pigment from heated LvPBP75 showed a λ max at 481nm in acetone. The significant red-colour change temperatures were detected at 30 and 80°C, respectively. Based on the determined amino acid fragments, a full-length cDNA of LvPBP75 was cloned and sequenced. The ORF encodes a protein of 662 amino acids having 80% identity with penaeidae hemocyanin. These results strongly suggest a novel function of hemocyanin, namely binding with pigment, and its involvement in L. vannamei shell colour change. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. IDENTIFICATION OF A LOCAL PROBIOTIC BACTERIUM USING 16S rRNA GENE SEQUENCE THAT WAS USED FOR FIELD TRIAL TO ENHANCED WHITELEG SHRIMP (Litopenaeus vannamei SURVIVAL

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    Tb. Haeru Rahayu

    2015-12-01

    Full Text Available The use of local probiotics in the culture of aquatic organisms is increasing with the demand for more environmental-friendly aquaculture practices. The local bacterium isolate considered as a probiotic was added into the water of whiteleg shrimp (Litopenaeus vannamei culture in a field trial. Four rectangular plastic ponds (ca. 20 m x 30 m per pond were used for 100 days experimentation for six consecutive crops in two years experiment. Survival, harvest size, feed conversion ratio (FCR and Vibrio bacterial count was compared with those of shrimp receiving and none of local isolate. Identification based on 16S rRNA gene sequence shown those isolate was Bacillus pumilus strain DURCK14 with 99% homology. Water shrimp pond added a local isolate had significantly higher survival at about 10.0% to 11.7% than shrimp without added the isolate (p<0.05, and better FCR, but no significant different in shrimp harvest size. Vibrio bacterial was undetected by total plate count. Moreover, it shown better projected yields on an annual basis (three crops per year.

  15. Susceptibility to an inoculum of infectious hypodermal and haematopoietic necrosis virus (IHHNV) in three batches of whiteleg shrimp Litopenaeus vannamei (Boone, 1931)

    Science.gov (United States)

    Escobedo-Bonilla, César Marcial; Rangel, José Luis Ibarra

    2014-01-01

    Abstract The present study evaluated the susceptibility of three different batches of whiteleg shrimp Litopenaeus vannamei from Mexico to an inoculum of infectious hypodermal and haematopoietic necrosis virus (IHHNV). Each of the three shrimp batches came from a different hatchery. Because of their origin, it was possible that the genetic makeup of these batches was different among each other. The three batches tested showed differences in IHHNV susceptibility. Here, susceptibility is defined as the capacity of the host to become infected, and it can be measured by the infectivity titer. Susceptibility to IHHNV was observed in decreasing order in shrimp from batch 1 (hatchery from El Rosario, Sinaloa), batch 3 (hatchery from Nayarit) and batch 2 (hatchery from El Walamo, Sinaloa), respectively. The largest susceptibility difference between batches was 5012 times, and that between early and late juveniles from the same batch was 25 times. These results indicate that within a species, susceptibility to a pathogen such as IHHNV can have large differences. Susceptibility to pathogens is an important trait to consider before performing studies on pathogenesis. It may influence virological parameters such as speed of replication, pathogenicity and virus titer. In order to evaluate the potential use of IHHNV as a natural control agent against white spot syndrome virus (WSSV), it is necessary to know host susceptibility and the kinetics of IHHNV infection. These features can help to determine the conditions in which IHHNV could be used as antagonist in a WSSV infection. PMID:25561847

  16. Effects of dietary inulin and mannan oligosaccharide on immune related genes expression and disease resistance of Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Li, Yun; Liu, Hong; Dai, Xilin; Li, Jingjing; Ding, Fujiang

    2018-02-19

    The effects of inulin and mannan oligosaccharide (MOS) at different doses (2.5, 4 and 10 mg/g) in singular or combined diet on growth rate, immune related genes expression, and resistance to white spot syndrome virus (WSSV) and Vibrio alginolyticus in Pacific white shrimp (Litopenaeus vannamei) were investigated. At the end of 28-day singular feeding experiment, the highest values of specific growth rate (SGR) and the expression of toll-like receptor1, 2 and 3 (TLR1, 2, 3), signal transducer and activator of transcription (STAT), crustin, anti-lipopolysaccharide factor (ALF) as well as prophenoloxidase (proPO) were observed in shrimp individually fed with 5 mg/g dietary inulin or MOS, respectively. Compared with individual treatments, diet containing combined prebiotics (5 mg/g inulin and MOS) significantly improved the expression of TLRs, STAT, proPO, crustin and ALF in L. vannamei after four-week feeding. Additionally, Pacific white shrimp fed with combined dietary prebiotics showed significantly higher expression of immune related genes and lower cumulative mortality in WSSV and Vibrio alginolyticus challenges, compared to the singular feeding groups and control. These results in the present study demonstrated that the combined supplementation of inulin (5 mg/g) and MOS (5 mg/g) remarkably enhanced innate immune response and pathogen resistance of shrimp, and should be considered as a promising immunostimulatory additive for the culture of Pacific white shrimp. Copyright © 2018 Elsevier Ltd. All rights reserved.

  17. Effects of different dietary lipid level on the growth, survival and immune-relating genes expression in Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Zhang, Sheng-peng; Li, Jin-feng; Wu, Xiao-chun; Zhong, Wei-jing; Xian, Jian-an; Liao, Shao-an; Miao, Yu-tao; Wang, An-li

    2013-05-01

    Five feeding trials based on the isonitrogenous and isoenergetic experimental diets containing 34% protein, 6%, 8%, 10%, 12% or 14% lipid respectively in the circulating water culture system for both 30 and 60 days were conducted to investigate the effect of the dietary lipid level on the growth and immunity in white shirmp, Litopenaeus vannamei adults. The body weight and specific growth rate of white shrimp in different treatments indicated that shrimps fed the diet of 12% lipid level for 30d and 10% lipid level for 60d had the best developmental status. The ability of respiratory burst in hemocytes was improved as the increase of dietary lipid level. The transcripts of LGBP and pPO were sensitive to the dietary lipid in hemocyte and hepatopancreas respectively. The activities of CAT, GPx and AKP were increased to a certain extend according to dietary lipid level. Qualification of MDA showed the lowest level in the sample subjected to 12% lipid level diet, indicating an optimal utilization of the dietary lipid and an efficient clearance of MDA in vivo. These results suggested that dietary lipid level of 10-12% significantly tunes the growth and enhance the immune abilities mainly via ROS pathway of L. vannamei. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. The Diversity of Vibrios Associated with Vibriosis in Pacific White Shrimp (Litopenaeus vannamei) from Extensive Shrimp Pond in Kendal District, Indonesia

    Science.gov (United States)

    Sarjito; Harjuno Condro Haditomo, Alfabetian; Desrina; Djunaedi, Ali; Budi Prayitno, Slamet

    2018-02-01

    Vibriosis out breaks frequently occur in extensive shrimps farming. The study were commenced to find out the clinical signs of white shrimp that was infected by the Vibrio and to identify the bacterial associated with vibriosis in the pacific white shrimp, Litopenaeus vannamei. Bacterial isolates were gained from hepatopancreas and telson of moribund shrimps that were collected from extensive shrimp ponds of Kendal District, Indonesia and cultured on Thiosulfate Citrate Bile Salts Sucrose Agar (TCBSA). Isolates were clustered and identified using repetitive sequence-based polymerase chain reaction (rep-PCR). Three representative isolates (SJV 03, SJV 05 and SJV 19) were amplified with PCR using primers for 16S rRNA, and sequence for further identification. The clinical signs of shrimps affected by vibrio were pale hepatopancreas, weak of telson, dark and reddish coloration of smouth, patches of red colour in part of the body on the carapace, periopods, pleuopods, and telson. A total of 19 isolates were obtained and belong to three groups of genus Vibrios. Result of the 16S DNA sequence analysis, the vibrio found in this study related to vibriosis in white shrimps from extensive shrimp ponds of Kendal were closely related to Vibrio harveyi (SJV 03); V. parahaemolyticus (SJV 05) and V. alginolyticus (SJV 19).

  19. Passive Immune-Protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus Infections with Anti-Vibrio Egg Yolk (IgY)-Encapsulated Feed.

    Science.gov (United States)

    Gao, Xiaojian; Zhang, Xiaojun; Lin, Li; Yao, Dongrui; Sun, Jingjing; Du, Xuedi; Li, Xiumei; Zhang, Yue

    2016-05-17

    Vibrio spp. are major causes of mortality in white shrimp (Litopenaeus vannamei) which is lacking adaptive immunity. Passive immunization with a specific egg yolk antibody (IgY) is a potential method for the protection of shrimp against vibriosis. In this study, immune effects of the specific egg yolk powders (IgY) against both V. harveyi and V. parahaemolyticus on white shrimp were evaluated. The egg yolk powders against V. harveyi and V. parahaemolyticus for passive immunization of white shrimp were prepared, while a tube agglutination assay and an indirect enzyme-linked immunosorbent assay (ELISA) were used for detection of IgY titer. Anti-Vibrio egg yolk was encapsulated by β-cyclodextrin, which could keep the activity of the antibody in the gastrointestinal tract of shrimp. The results showed that the anti-Vibrio egg powders had an inhibiting effect on V. harveyi and V. parahaemolyticus in vitro. Lower mortality of infected zoeae, mysis, and postlarva was observed in groups fed with anti-Vibrio egg powders, compared with those fed with normal egg powders. The bacterial load in postlarva fed with specific egg powders in seeding ponds was significantly lower than those fed with normal egg powders in seeding ponds. These results show that passive immunization by oral administration with specific egg yolk powders (IgY) may provide a valuable protection of vibrio infections in white shrimp.

  20. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

    Science.gov (United States)

    Xie, Shijun; Li, Fuhua; Zhang, Xiaojun; Zhang, Jiquan; Xiang, Jianhai

    2017-11-01

    The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at 27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

  1. RNA-Seq reveals the dynamic and diverse features of digestive enzymes during early development of Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Wei, Jiankai; Zhang, Xiaojun; Yu, Yang; Li, Fuhua; Xiang, Jianhai

    2014-09-01

    The Pacific white shrimp (Litopenaeus vannamei), with high commercial value, has a typical metamorphosis pattern by going through embryo, nauplius, zoea, mysis and postlarvae during early development. Its diets change continually in this period, and a high mortality of larvae also occurs in this period. Since there is a close relationship between diets and digestive enzymes, a comprehensive investigation about the types and expression patterns of all digestive enzyme genes during early development of L. vannamei is of considerable significance for shrimp diets and larvae culture. Using RNA-Seq data, the types and expression characteristics of the digestive enzyme genes were analyzed during five different development stages (embryo, nauplius, zoea, mysis and postlarvae) in L. vannamei. Among the obtained 66,815 unigenes, 296 were annotated as 16 different digestive enzymes including five types of carbohydrase, seven types of peptidase and four types of lipase. Such a diverse suite of enzymes illustrated the capacity of L. vannamei to exploit varied diets to fit their nutritional requirements. The analysis of their dynamic expression patterns during development also indicated the importance of transcriptional regulation to adapt to the diet transition. Our study revealed the diverse and dynamic features of digestive enzymes during early development of L. vannamei. These results would provide support to better understand the physiological changes during diet transition. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. SUBSTITUIÇÃO DE RAÇÃO NO CRESCIMENTO DE JUVENIS DO CAMARÃO MARINHO Litopenaeus vannamei EM LABORATÓRIO

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    CIBELE SOARES PONTES

    2010-01-01

    Full Text Available To evaluate the effect of the temporary replacement of shrimp ration (RC by chicken ration (RF in the culture of Litopenaeus vannamei shrimp, juvenils (1.07 ± 0.26 g, were cultivated (52/m2 for 30 days in 50 L boxes, with constant aeration, 0% of water exchange and fed with 10% of its biomass/day at 08:00 and 16:00 h, according to the treatments: (1 RC; (2 1 week RC + 1 week RF, alternate; (3 RF and (4 1 day RF + 1 day RC, alternate; with 5 repetitions each. There was no difference (P <0.05 in the weight of shrimp on the basis of treatments applied at the beginning (Kruskal-Wallis, P = 0003, 15 days (Kruskal-Wallis, P = 0.98 and at 30 days (Kruskal-Wallis, P = 0.07 to experiment. The Specific Growth Rate (rm ANOVA, P = 0.28 and survival (Friedman, P = 0,34 the animals were significantly similar, indicating that these parameters were not affected by treatments, with the possible replacement of shrimp ration by chicken ration, as a measure of containment of expenditure, for short intervals of time. Studies are needed to validate these results in earth ponds.

  3. Evaluation of the presence of Vibrio parahaemolyticus in white shrimp (Litopenaeus vannamei estuarine-wild from southern Sinaloa and northern Nayarit by microbiological analysis and PCR

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    Méndez-Gómez, E.

    2014-01-01

    Full Text Available Given the incidence of human poisonings attributed to raw shrimp consumption in southern of Sinaloa and northern of Nayarit in recent years, white shrimp (Litopenaeus vannamei was sampled from three wildestuaries where it’s been captured and one sample was taken from a sale center in order to determine the possible presence of toxigenic Vibrio parahaemolyticus, from May to December of 2012. Samples were analyzed by Biochemical test and Polymerase Chain Reaction (PCR, they were also tested for the specific an toxicological identification, using molecular oligo nucleotides markers tlh, tdh and trh. The number of positives results were recorded to a table of most probable number (MPN.The number of samples positive for V. parahaemolyticus, weren’t toxigenic and it was demonstrated that they were below the limit established in the Mexican Official Standard NOM-242-SSA1-2009. In conclusion, the present study revealed that consumption of raw shrimp from the dates and sites sampled did not show to be a risk for human gastrointestinal diseases.

  4. Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei

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    Escobedo-Lozano, A.Y.

    2014-01-01

    Full Text Available The need of new food sources to satisfy human requirements forces researchers to study any possible alternative supplies. Therefore this study aimed to explore the quality and digestibility evaluation of the protein fractions obtained as a by-product of processing the shrimp exoskeleton Litopenaeus vannamei. Shrimp exoskeletons were washed, they were dried, mincedand they were brought under acid hydrolysis and alkaline hydrolysis; released proteins were then precipitated and characterized. 496 g of demineralized exoskeleton were obtained from 1 kg of shrimp shell; out of these, 376 g corresponded to chitin and 120 g corresponded to protein Total protein content was 33.80 ± 0.34 %. The digestible protein fractions were 26.7 g and 92.1 g were insoluble proteins. Of this latter fraction, the largest proportion belonged to the scleroprotein type. The amino acid profile analysis from the recovered proteins indicated that there were present nine amino acids out of the ten essential ones, being leucine the one with the highest proportion. In vitro digestibility was shown to be up to an 83.7 %. Therefore, it was possible to conclude that the recovery of digestibleproteins from shrimp exoskeleton may be useful in diet formulation.

  5. Growth and feed efficiency of juvenile shrimp Litopenaeus vannamei fed formulated diets containing different levels of poultry by-product meal

    Science.gov (United States)

    Chi, Shuyan; Tan, Beiping; Mai, Kangsen; Zheng, Shixuan

    2009-12-01

    This feeding trial was conducted to evaluate the potential of poultry by-product meal (PBM) as a protein source in the culture of Litopenaeus vannamei. Seven isonitrogenous and isoenergetic diets were formulated to near to commercial diet with about 40% protein and 7.5% lipid. Fish meal was replaced by 0, 30%, 40%, 50%, 60%, 70% and 100% of PBM (diets 1-7). The diet with 100% fish meal was used as a control (diet 1). Post-larvae were reared in an indoor semi-closed re-circulating system. Each dietary treatment was tested in 4 replicate tanks (260 L) of 40 shrimp, arranged in a completely randomized design. The shrimps were hand-fed for three times a day to near-satiation (0700, 1200 and 1800) for 60 d. Percentage weight gain, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition of shrimps were measured. There were no significant differences ( P>0.05) in growth performance among shrimps fed diets 1-5 (0-60% fish meal replacement). However, shrimps fed diet 7 (100% fish meal replacement) had significantly lower ( P0.05) among different experimental diets. No differences in body composition were found among shrimps fed different diets. These results showed that up to 70% of fish meal protein can be replaced by PBM without adversely affecting the growth, survival, FCR, PER and body composition of Litop enaeus vannamei.

  6. Inhibition of Taura syndrome virus replication in Litopenaeus vannamei through silencing the LvRab7 gene using double-stranded RNA.

    Science.gov (United States)

    Ongvarrasopone, Chalermporn; Saejia, Pipop; Chanasakulniyom, Mayuree; Panyim, Sakol

    2011-07-01

    Taura syndrome virus (TSV) is a major cause of high mortality in Pacific white shrimp (Litopenaeus vannamei, Lv). Previously, silencing of Penaeus monodon Rab7 (PmRab7) by injecting double-stranded RNA corresponding to PmRab7 (dsRNA-PmRab7) prevented white spot syndrome virus or yellow head virus infection. Rab7 is proposed to be involved in intracellular trafficking of the viruses. This study aimed to investigate whether knockdown of Rab7 in L. vannamei by dsRNA-PmRab7 could inhibit replication of TSV. RNA interference (RNAi) technology using dsRNA targeting the LvRab7 gene was used to silence the mRNA expression of LvRab7. The silencing of the LvRab7 gene inhibited TSV replication dramatically when compared to groups receiving dsRNA-GFP or NaCl. This is the first demonstration that dsRNA targeting the endogenous shrimp gene LvRab7 strongly reduces TSV replication. It provides further evidence that LvRab7 is involved in the endosomal trafficking pathway of viruses infecting penaeid shrimp.

  7. Effect of the diet traditional and non-traditional on the respiration and excretion in larvae of white shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    María Alejandra Medina-Jasso

    2015-11-01

    Full Text Available Objetive. It was studied the respiration and ammoniacal excretion of zoeas and mysis of Litopenaeus vannamei fed with the diet used traditionally (of microalgae and nauplios of artemia and another alternative (not traditional of microalgae with rotifers. Materials and methods. After four hours the oxygen consumption and ammonia excretion in BOD bottles with 60 larvae (closed respirometers was estimated. The concentrations of O2 and NH4 + were measured with an electrode polarográfico in the first case and with the indophenol blue technique for the second. Results. In zoea, oxygen consumption increased with development and showed statistical differences (p=0.023. In mysis, the oxygen consumption were significance in the traditional diet, whereas no differences were alternative (p=0.003. In both stages for the ammoniacal excretion increased development stage and there were detected statistical difference (p<0.001, although to the diets were not noticed significant differences. Conclusions. A higher energy absorption for zoea (I, II y III what mysis (I, II y III larvae was obtained, this is likely an interaction between rates of respiration and excretion caused by variations in the efficiency of absorption by the larvae. The weights obtained in both larvae were not supplied with differences between diets.

  8. 2,3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannamei.

    Science.gov (United States)

    Cavalcante, Rômulo S; Brito, Adriana S; Palhares, Lais C G F; Lima, Marcelo A; Cavalheiro, Renan P; Nader, Helena B; Sassaki, Guilherme L; Chavante, Suely F

    2018-03-01

    The occurrence of a natural and unmodified highly sulfated chondroitin sulfate from Litopenaeus vannamei heads (sCS) is herein reported. Its partial digestion by Chondroitinases AC and ABC together with its electrophoretic migration profile revealed it as a highly sulfated chondroitin sulfate despite its average molecular weight being similar to CSA. Using orthogonal 1D/2D NMR experiments, the anomeric signals (δ 4.62/106.0) corresponding to unusual 2,3-di-O-Sulfo-GlcA (∼36%), U3 3S (δ 4.42/84.1, ∼63%) and U2 2S (4.12/80.1, ∼50%) substitutions were confirmed. In addition, non-sulfated GlcA (δ 4.5/106.3) linked to 4-O- (A1 4S , 36%) or 6-O-Sulfo (A1 6S , 28%) GalNAc (δ 4.64/103.5) was observed. Although the biological role of sCS in shrimp is unknown, its influence on hemostasis was also demonstrated. The sCS identification brings to light new questions about the hierarchical model of GAGs biosynthesis and contributes to the better understanding of the subtle relationship between GAGs structure and function. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. A study on the meat and bone meal and poultry by-product meal as protein substitutes of fish meal in practical diets for Litopenaeus vannamei juveniles

    Science.gov (United States)

    Zhu, Wei; Mai, Kangsen; Zhang, Baigang; Wang, Fuzhen; Yu, Yu

    2004-10-01

    A study was conducted to evaluate the effects of meat and bone meal (MBM) and poultry by-product meal (PBM) as the replacement of fish meal in the diets on the growth performance, survival and apparent digestibility coefficients (ADC) of Litopenaeus vannamei. The basal diets were formulated with 22% fish meal and other ingredients which provided about 40% protein and 9% lipid in the diet. The experimental diets included MBM or PBM to replace 0, 20%, 40%, 60% and 80% of total fish meal respectively. All diets were iso-nitrogenous and isocaloric in gross terms. The results showed that there were no significant differences (Pτ;0.05) in growth performance and ADC among the treatments fed with the diets in which 0 60% fish meal had been replaced with MBM, while the percent weight gain (WG, %), body length gain (BLG, %) and ADC significantly decreased when the MBM was up to 80% of the fish meal. There were no significant differences (Pτ;0.05) in growth performance and ADC among all the treatments fed with the diets in which 0 80% fish meal had been replaced with PBM.

  10. Survey of protozoan, helminth and viral infections in shrimp Litopenaeus setiferus and prawn Macrobrachium acanthurus native to the Jamapa River region, Mexico.

    Science.gov (United States)

    Domínguez-Machín, Magda E; Hernández-Vergara, Martha P; Jiménez-García, Isabel; Simá-Alvarez, Raúl; Rodríguez-Canul, Rossanna

    2011-09-09

    We surveyed protozoan and metazoan parasites as well as white spot syndrome virus (WSSV) and infectious hypodermal hematopoietic necrosis virus (IHHNV) in white shrimp Litopenaeus setiferus and the palaemonid prawn Macrobrachium acanthurus native to the lower Jamapa River region of Veracruz, Mexico. The presence of parasites and the infection parameters were evaluated in 113 palaemonid prawns collected during the northwind (n = 45), rainy (n = 38) and dry seasons (n = 30) between October 2007 and July 2008, and in 91 shrimp collected in the rainy season between May and June 2008. In L. setiferus, ciliates of the subclass Apostomatia (Ascophrys sp.) were evident in gills, and third-stage larvae of the nematode Physocephalus sexalatus were evident in the stomach. Cestodes of the genus Prochristianella were evident in the hepatopancreas, while some gregarines of the genus Nematopsis, as well as unidentified larval cestodes, were observed in the intestine. Histology identified Ascophrys sp. in association with gill necrosis and tissue melanization. Slight inflammation was observed in intestinal epithelium near cestode larvae. In M. acanthurus, epibionts of the protozoans Epistylis sp., Acineta sp. and Lagenophrys sp. were observed under uropods, periopods and pleopods. An unidentified ciliate of the Apostomatia was also found in the gills, and Nematopsis was identified in the intestine. No histopathology was observed in association with these parasites. Moreover, neither WSSV nor IHHNV were detected by the polymerase chain reaction (PCR) in any of the L. setiferus or M. acanthurus analysed.

  11. Effect of addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange

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    Yllana Ferreira-Marinho

    2014-07-01

    Full Text Available The aim of this study was to evaluate the effect of the addition of Navicula sp. on plankton composition and postlarvae growth of Litopenaeus vannamei reared in culture tanks with zero water exchange systems. Four treatments were considered: zero water exchange (ZWE; ZWE with the addition of feed (ZWE-F; ZWE with the addition of Navicula sp. (ZWE-N and ZWE with the addition of feed and Navicula sp. (ZWE-FN, all in triplicate. Shrimp of 17.7 ± 0.02 mg were stocked at a density of 2500 shrimp m-3 and microalgae added on the 1st, 5th and 15th day at a density of 5x10(4 cell mL-1. The shrimp were fed a commercial feed composed by 42% crude protein four times a day except in the ZWE treatment. For data analysis we used Cochran, Shapiro-Wilk, ANOVA, Tukey and Student-t tests (P < 0.05. The most frequent genera were: Anabaena, Arcella, Asplanchma, Bosmina, Brachionus, Cylindrotheca, Daphnia, Fragilaria, Hemiaulus, Keratella, Orthoseira, Oscillatoria, Phymatodocis, Rhabdonema, Skeletonema, Sckizothrix and Ulothrix. Significant differences between treatments were observed for TAN, NO2-N, alkalinity, final weight, weight gain, final biomass, biomass gain, feed conversion ratio, specific growth rate and survival. The ZWE-FN treatment showed better production parameters, indicating the benefits of the addition of Navicula sp. as a natural food source for L. vannamei postlarvae in zero water exchange systems.

  12. Productividad de asociaciones de pasto ovillo (Dactylis glomerata L., ballico perenne (Lolium perenne L. y trébol blanco (Trifolium repens L.

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    Ever del J. Flores Santiago

    2015-01-01

    Full Text Available El objetivo del estudio fue evaluar el rendimiento de forraje de los pastos, ovillo (Dactylis glomerata L. y ballicoperenne (Lolium perenne L. en monocultivo y asociados con trébol blanco (Trifolium repens L., en su segundo añode crecimiento. Los tratamientos evaluados fueron: 20:40:40; 00:50:50; 40:20:40; 50:00:50; 20:70:10; 70:20:10,40:40:20, 100:00:00 y 00:100:00 de pasto ovillo, ballico perenne y trébol blanco, respectivamente. El diseño experimentalfue bloques al azar con tres repeticiones. Se midió rendimiento de forraje, altura de planta, radiación interceptada(RI, % y composición botánica del forraje cosechado (%. Los tratamientos se defoliaron por ovinos (Suffolk xDorset de acuerdo con la estación del año (cada cinco, seis y cuatro semanas en otoño, invierno y primavera-verano,respectivamente. Las asociaciones 00:50:50, 20:40:40, y 40:20:20 tuvieron una diferenciación mayor enrendimiento anual produciendo más del 22 % que los monocultivos de pastos y la asociación 40:40:20 quepresentaron los menores rendimientos (en promedio 15,027 kg MS ha-1. La producción estacional tuvo diferente(P<0.05 aportación durante el año; en otoño-invierno se produjo el 40 % y en primavera-verano 60 %. El trébolblanco fue la especie con mayor porcentaje del forraje cosechado (44 % seguida por pasto ovillo (39 % yballico perenne (17 %. En conclusión, algunas asociaciones superaron el rendimiento de los pastos en monocultivo,existiendo diferencias también en la distribución estacional de la producción de forraje. La intercepción deradiación y altura de la planta son indicativos del rendimiento, y por tanto del momento de cosecha.

  13. GEOARQUEOLOGÍA, RADIOCARBONO Y CRONOESTRATIGRAFÍA DEL YACIMIENTO SOLUTRENSE DE LA CUEVA DE AMBROSIO (VÉLEZ-BLANCO, ALMERÍA, ESPAÑA

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    Jesús F. Jordá Pardo

    2013-09-01

    Full Text Available La Cueva de Ambrosio (Vélez-Blanco, Almería, España es un yacimiento situado en el extremo SE de la Península Ibérica que presenta una secuencia estratigráfica en la que se han excavado tres unidades sedimentarias con materiales del Solutrense. En este trabajo estudiamos la secuencia desde la perspectiva geoarqueológica y cronoestratigráfica. El estudio geoarqueológico ha permitido distinguir dos unidades litoestratigráficas con diferente significado, una inferior generada por una colada de fango y otra superior formada por procesos de gelifracción que contiene las ocupaciones solutrenses. Las últimas dataciones radiocarbónicas obtenidas permiten clarificar la posición de la secuencia en la escala cronoestratigráfica del Pleistoceno superior. La calibración de las nuevas fechas 14C del nivel IV (Solutrense superior y del nivel II (Solutrense superior evolucionado permiten situar estos momentos entre el final del GS 3 (final del OIS 3 y el final del GI 2, con una importante ocupación (nivel II al final del evento H-2, coincidiendo con el interestadial que precede al último Máximo Glacial (GS 2.La Cueva de Ambrosio (Vélez-Blanco, Almería, Spain is located in the Southeast of the Iberian Peninsula. It is a karstic cavity with rock shelter morphology that contains a stratigraphic sequence composed of three sedimentary units with Solutrean materials. In this work we study the sequence from the geoarchaeological and chronostratigraphical perspective. The geoarchaeological study has allowed us to distinguish two litostratigraphic units, the lower generated by a mud flow and the upper formed by gelifraction processes which contains the Solutrean occupations. The last radiocarbon dates from the Solutrean of La Cueva de Ambrosio allow the clarification of its position in the Upper Pleistocene chronostratigraphic scale. The calibration of the new 14C dates from the level IV (Upper Solutrean and the level II (Upper Evolved Solutrean

  14. Insights into the genesis of the epithermal Au-Ag mineralization at Rio Blanco in the Cordillera Occidental of southwestern Ecuador: Constraints from U-Pb and Ar/Ar geochronology

    Science.gov (United States)

    Bineli Betsi, Thierry; Ponce, Miguel; Chiaradia, Massimo; Ulianov, Alex; Camacho, Alfredo

    2017-12-01

    The genesis of the Au-Ag mineralization at Rio Blanco in the Cordillera Occidental (Western Cordillera) of southwest of Ecuador is here constrained. This was done by investigating the temporal and by inference the genetic relationship between the Au-Ag mineralization and the spatially associated magmatic host rocks using zircon U-Pb [chemical abrasion (CA) IDTIMS and laser ablation (LA) ICPMS] and adularia 40Ar/39Ar geochronology. Whereas volcanics hosting the Au-Au mineralization range in age from 37.35 ± 0.30 to 33.09 ± 0.20 Ma (Late Eocene-Early Oligocene), the spatially associated intrusions are of at least two discontinuous phases of magmatism and these include: (i) Late Eocene intrusions that range in age from 35.77 ± 0.19 to 36.03 ± 0.19 Ma, and; (ii) Miocene intrusions of 15.58 ± 0.04 Ma. The 40Ar/39Ar age of adularia from a Bonanza Au (334 g/t)-Ag (2060 g/t)-bearing epithermal vein is bracketed between 14.3 and 14.9 Ma. The temporal relationship between adularia and by inference mineralization and the spatially associated rocks therefore rules out any temporal link between the Late Eocene-Early Oligocene Rio Blanco Formation, but rather favors a possible genetic relationship between the Rio Blanco Au-Ag mineralization and the Miocene intrusions. The determined Rio Blanco Au-Ag mineralization age is consistent with the established Oligocene-Miocene period of ore deposits in Ecuador and coincides with the extensional tectonic event, which developed intramontane basins in southern Ecuador.

  15. Evaluation of the nutritional quality of Chaetoceros muelleri Schütt (Chaetocerotales: Chaetocerotaceae and Isochrysis sp. (Isochrysidales: isochrysidaceae grown outdoors for the larval development of Litopenaeus vannamei (Boone, 1931 (Decapoda: Penaeidae

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    Rodríguez Erika O.

    2012-01-01

    Full Text Available The biomass, proximal composition and fatty acid profile of Isochrysis sp., Chaetoceros muelleri and their mixture, grown under greenhouse conditions, were evaluated. The nutritional value of both species supplied as the monoalgal (Chaetoceros muelleri: Diet I, and Isochrysis sp. Diet II and mixed diet (Diet III for larval Litopenaeus vannamei was also assessed on the basis of the development and biochemical composition of the larvae. The highest protein levels were obtained in Diets I and II (40% and 35%, respectively. No significant differences in larval survival were found among the diets; however, larvae fed on Diet II had the lowest mean larval length.

  16. Cultivo de Camarão Branco Litopenaeus Vannamei (Boone, 1931) com a Macro-alga Ulva Lacuata Linneaus (Chlorophyta) no Tratamento de Efluentes em Sistema Fechado de Recirculação

    OpenAIRE

    Alencar, J. R.; Horta Junior, P. A.; Celino, Joil José

    2010-01-01

    p. 117-137 Objetivou-se explorar o potencial da macroalga Ulva lactuca para avaliar o cultivo integrado com Litopenaeus vannamei em sistema fechado de recirculação. U. lactuca foi cultivada em recipientes de Erlenmeyer de 500mL, contendo água de efluentes de uma fazenda de camarões marinhos sob diferentes concentrações de NH4 + e PO4 3-. No sistema de recirculação, utilizaram-se camarões, distribuídos em seis tanques de 500L. As algas foram cultivadas em 3 tanques de 250L...

  17. Efectos combinados de las vitaminas c y e dietéticas en la inmunorespuesta del juvenil litopenaeus vannamei antes y después de la suplementación con glucanos

    OpenAIRE

    Molina, César; Rodríguez, Jenny; Echeverría, Fabrizio

    2001-01-01

    Efectos combinados de las vitaminas C y E dietéticas en la inmunorespuesta del juvenil Litopenaeus vannamei antes y después de la suplementación con glucanos Se considera que el estado nutricional tiene una influencia importante en la salud y en la capacidad de los animales para resistir enfermedades. Estudios relacionando la nutrición con la función inmune han sido mayormente desarrollados en peces sin embargo su rol en la resistencia a enfermedades es pobremente entendido.

  18. Bacillus NP5 Improves Growth Performance and Resistance Against Infectious Myonecrosis Virus in White Shrimp (Litopenaeus vannamei (Bacillus NP5 Meningkatkan Pertumbuhan dan Ketahanan Terhadap Infeksi Virus Myonecrosis pada Udang Putih (L. vannamei

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    Widanarni Widanarni

    2015-07-01

    Full Text Available Infectious Myonecrosis (IMN merupakan salah satu penyakit yang sering menyerang udang vaname. Probiotik banyak digunakan pada budidaya udang karena terbukti mampu mengurangi serangan penyakit pada udang. Penelitian ini bertujuan untuk menguji pengaruh pemberian probiotik Bacillus NP5 melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi udang vaname terhadap infeksi Infectious Myonecrosis Virus (IMNV. Udang vaname Litopenaeus vannamei (2.41±0.07 g ekor-1 diberi pakan yang disuplementasi probiotik Bacillus NP5 dengan dosis yang berbeda, 102 CFU.g-1 (A, 104 CFU.g-1 (B, 106 CFU.g-1 (C, dan kontrol tanpa suplementasi probiotik (kontrol negatif, KN; kontrol positif, KP selama 30 hari dan dengan tiga ulangan untuk masing-masing dosis, kemudian KP, perlakuan A, B, dan C diuji tantang secara intramuskular dengan IMNV (100 µl.ekor-1. Hasil penelitian ini menunjukkan bahwa udang vaname yang diberi pakan dengan suplementasi probiotik mempunyai laju pertumbuhan harian (LPH, rasio konversi pakan (RKP, dan respons imun yang lebih tinggi. Udang tersebut juga mempunyai total hemocyte count (THC dan resistensi terhadap IMNV yang lebih tinggi dibandingkan kontrol positif. Konsentrasi probiotik 106 CFU.g-1 memberikan hasil terbaik dalam meningkatkan pertumbuhan, respon imun, dan resistensi udang vaname terhadap infeksi IMNV. Kata kunci: probiotik, Bacillus NP5, Litopenaeus vannamei, pertumbuhan, IMNV Infectious Myonecrosis (IMN is one of the most prevalent white shrimp diseases. Probiotics are widely used in shrimp cultivation because they have been proven to reduce shrimp disease outbreak. This study aimed to observe the effect of oraly administered probiotic Bacillus NP5 on the white shrimp's growth performance, immune response, and resistance to Infectious Myonecrosis Virus (IMNV infection. White shrimp Litopenaeus vannamei (2.41±0.07 g individual-1 were fed with a feed supplemented with different doses of the probiotic Bacillus NP5, i

  19. Farinha dos resíduos do camarão Litopenaeus vannamei: caracterização e utilização na formulação de hambúrguer

    OpenAIRE

    Suzanne Florentino da Silva Chaves Damasceno, Karla

    2007-01-01

    O potencial brasileiro para a produção e exportação de camarão Litopenaeus vannamei e o grande volume de resíduo gerado pelo seu beneficiamento motivaram a presente pesquisa com o objetivo de contribuir para o aproveitamento destes e incentivar a utilização da farinha de resíduo de camarão em produtos alimentícios. Foram realizados estudos por meio de planejamento fatorial 23, para definir os parâmetros de fabricação da farinha a partir de resíduos do beneficiamento do camarão ...

  20. Adaptación de Cultivares de Ajo Morado y Blanco (Allium sativum L.) en Acatlán, Guerrero, México. Adaptación de Cultivares de Ajo Morado y Blanco (Allium sativum L.) en Acatlán, Guerrero, México

    OpenAIRE

    Efrén Cevallos Ruiz; Sergio Ayvar Serna; Enrique Palemón Alberto; Luis Pérez Moreno

    2012-01-01

    En el estado de Guerrero, México, el ajo se cultiva en la región Centro, en los municipios de Chilapa y Zitlala, donde los productores acostumbran sembrar el genotipo criollo adaptado a las condiciones climáticas de la región. El objetivo fue estudiar la adaptación del cultivo del ajo de los tipos morado y blanco a la región Centro de Guerrero por su rendimiento y calidad, ya sea para el mercado nacional o el de exportación. La investigación se llevó a cabo durante el ciclo otoño-invierno 20...

  1. Epidemiología de la enfermedad de Chagas en el municipio Andrés Eloy Blanco, Lara, Venezuela: infestación triatomínica y seroprevalencia en humanos Epidemiology of Chagas disease in Andrés Eloy Blanco, Lara, Venezuela: triatomine infestation and human seroprevalence

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    Claudina Rodríguez-Bonfante

    2007-05-01

    Full Text Available Se realizó un despistaje serológico y recolección de vectores en cuatro comunidades rurales del municipio Andrés Eloy Blanco, Estado Lara, Venezuela. La muestra fue escogida en forma sistemática y aleatoria basada en conglomerados familiares. Se muestrearon 869 habitantes para determinar anticuerpos anti-Trypanosoma cruzi y anti-Leishmania sp. por inmunofluorescencia indirecta, aceptando como positivo diluciones > a 1:32 para anticuerpos anti-T. cruzi no reactivos para antígenos de Leishmania sp., obteniendo una frecuencia de anticuerpos en la muestra de 6,9% (n = 60; de los cuales 46,66% son femeninos, 53,33% masculinos y 60% mayores de 40 años. Se observó que 5 (8,33% de los seropositivos eran menores de 10 años y 10 (16,66% menores de 20 años. Rhodnius prolixus y Panstrongylus geniculatus fueron los triatominos capturados, con índice de infestación de 1,9 y 10,54%, índice de colonización, del 0 y 18,18% en las viviendas infestadas e índice de infección a T. cruzi del 20 y 5,07%, respectivamente. Los resultados sugieren que existe una transmisión activa de la enfermedad de Chagas en el Municipio Andrés Eloy Blanco en las últimas dos décadas y que P. geniculatus está substituyendo a R. prolixus como vector de la enfermedad de Chagas.A seroepidemiological survey and vector captures were performed in four rural communities in Andrés Eloy Blanco, Lara State, Venezuela. Systematic random sampling was based on family clusters, with samples drawn from 869 individuals to determine anti-Trypanosoma cruzi and anti-Leishmania sp. antibodies by indirect immunofluorescence. Positive individuals were defined as > 1:32 for anti-T. cruzi antibody and non-reactive to Leishmania sp. antigen, revealing an antibody frequency of 6.9% (n = 60, of whom 46.66% were females and 53.33% males and 60% were over 39 years of age. Some 5 (8.33% seropositive individuals were under 10 years of age and 10 (16.66% under 20 years. Rhodnius prolixus and

  2. Aclimatação do camarão marinho Litopenaeus vannamei (Boone, 1931 à água doce com diferentes estratégias de alimentação e calagem - DOI: 10.4025/actascianimsci.v28i1.668 Acclimatization of the marine shrimp Litopenaeus vannamei (Boone, 1931 to the freshwater with different feeding and liming strategies - DOI: 10.4025/actascianimsci.v28i1.668

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    Bruno Leonardo da Silva Santos

    2006-01-01

    Full Text Available Pós-Larvas do camarão Litopenaeus vannamei, na fase de PL9-10 e PL25-26 foram aclimatadas à água doce (0,0 ‰, objetivando promover cultivos em regiões interioranas. Para isso, foram realizados dois experimentos conduzidos em aquários com capacidade útil de 44 litros e densidade de estocagem de 11,36 pós-larvas/L. No experimento 1, foram testadas diferentes concentrações de cal hidratada (0,018 g a 0,031 g e 2 dietas, formuladas à base de ração comercial para camarão e biomassa de artêmia. No experimento 2, foram testadas 5 dietas com diferentes concentrações de biomassa de artêmia (0% a 100%, inseridas em rações de peixes e camarões. No final do processo de aclimatação, a sobrevivência das póslarvas variou de 40,44% a 98,04%. Concluiu-se que pós-larvas do Litopenaeus vannamei podem ser aclimatadas à água doce e que o aumento da concentração de cal e de artêmia na ração melhoraram significativamente (p Litopenaeus vannamei postlarvae with 9-10 (PL9-10, 25-26 days old (PL25-26 were acclimated to freshwater (0 ppt for the purpose of attending the inland cultures. Two experiments were conducted in 44 liter aquariums with stocking density of 11.36 postlarvae/L. In experiment 01, different concentrations of hydrated lime (0.018 g a 0.031 g and two diets, formulated with commercial shrimp ration and artemia biomass, were tested. In experiment 02, five diets, with different concentrations of artemia biomass (0 to 100% inserted in fish and shrimp rations, were tested. At the end of the acclimatization process, the postlarvae survival varied from 40.44 to 98.04%. Results showed that Litopenaeus vannamei postlarvae can be acclimated to freshwater. Also, the increase of lime concentration and the artemia biomass in the ration improved (p < 0.05 the zootechnic parameters of the species in a significant way.

  3. CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco).

    Science.gov (United States)

    Li, Peng; Miao, Hongxia; Ma, Yuewen; Wang, Lu; Hu, Guibing; Ye, Zixing; Zhao, Jietang; Qin, Yonghua

    2015-09-09

    Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from "Wuzishatangju" (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of "Wuzishatangju".

  4. Heat shock transcription factors expression during fruit development and under hot air stress in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit.

    Science.gov (United States)

    Lin, Qiong; Jiang, Qing; Lin, Juanying; Wang, Dengliang; Li, Shaojia; Liu, Chunrong; Sun, Chongde; Chen, Kunsong

    2015-04-01

    Heat shock transcription factors (Hsfs) play a role in plant responses to stress. Citrus is an economically important fruit whose genome has been fully sequenced. So far, no detailed characterization of the Hsf gene family is available for citrus. A genome-wide analysis was carried out in Citrus clementina to identify Hsf genes, named CcHsfs. Eighteen CcHsfs were identified and classified into three main clades (clades A, B and C) according to the structural characteristics and the phylogenetic comparison with Arabidopsis and tomato. MEME motif analysis highlighted the conserved DBD and HR-A/B domains, which were similar to Hsf protein structures in other species. Gene expression analysis in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit identified 14 Hsf genes, named CrHsf, as important candidates for a role in fruit development and ripening, and showed seven genes to be expressed in response to hot air stress. CrHsfB2a and CrHsfB5 were considered to be important regulators of citrate content and showed variation in both developmentally-related and hot air-triggered citrate degradation processes. In summary, the data obtained from this investigation provides the basis for further study to dissect Hsf function during fruit development as well as in response to heat stress and also emphasizes the potential importance of CrHsfs in regulation of citrate metabolism in citrus fruit. Copyright © 2015 Elsevier B.V. All rights reserved.

  5. La Preparación Física Profesional en tabaqueros de la Fábrica Serafín Sánchez Valdivia en Arroyo Blanco.

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    Luis Bécquer Quesada

    2009-10-01

    Full Text Available La preparación física profesional es un proceso especializado y dirigido al desarrollo de las capacidades físicas, psíquicas y de hábitos motores en los trabajadores, lo que resulta imprescindible para elevar la efectividad y la capacidad de trabajo. Para el presente trabajo se elaboró un sistema de ejercicios (contracción muscular, flexibilidad para contribuir al mejoramiento de la preparación física profesional en los tabaqueros, de la fábrica Serafín Sánchez Valdivia en Arroyo Blanco. En esta investigación se tomó una población integrada por los 39 tabaqueros de la entidad y como muestra 10 de ellos, lo que representa el (25.64%. El Objeto de estudio de esta investigación es el Proceso de Preparación para la Salud de los Tabaqueros y como métodos se utilizó el Teórico (análisis-síntesis, histórico-lógico, inductivo-deductivo y Enfoque de sistema, Empíricos: la observación, encuesta, entrevista y Validación mediante consulta a especialistas.

  6. Gino Germani y la historia de la sociología en Argentina. Entrevista al sociólogo Alejandro Blanco

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    Jaime Eduardo Jaramillo Jiménez

    2011-07-01

    Full Text Available En la entrevista realizada a Alejandro Blanco, quien ha venido trabajando en una sociología y una historia de la sociología en América Latina, y particularmente en Argentina, se exploran, en un primer momento, algunas sincronías en el proceso de institucionalización de la sociología, en Argentina y Colombia, así como en otros países de la región. Posteriormente, la entrevista se enfoca en el papel y la figura de Gino Germani en la fundación de la Carrera de Sociología en la Universidad de Buenos Aires, ubicándolo en las discusiones y trayectoria del campo intelectual de su época. Por último, el entrevistado alude a los años sesenta en la sociología argentina, explorando el papel de algunas revistas e instituciones, nacionales e internacionales, en sus procesos de inicial consolidación y legitimación.

  7. Montes blancos, baldíos y realengos: aproximación a tres tipologías tradicionales de montes públicos en la provincia de Zaragoza

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    Pérez-Soba Díez del Corral, Ignacio

    2005-06-01

    Full Text Available The article analyses the meaning of three terms for common land that are very common in Aragon and other parts of Spain and which correspond to obsolete legislation or traditional names, namely, "montes blancos", "baldíos" and "realengos". The term "montes blancos" has often been used as a synonym of communal land or deforested land ('moorlands'. Regarding the first of these identifications, the article concludes that, in Aragon, the "montes blancos" arise both from lands that were originally held communally ("montes comunes", to use the terminology of the Ancient Régime and the wastelands ("baldíos" over which the boroughs came to hold an undisputed title vis-à-vis the State and which effectively became communal lands. However, subsequent enclosure processes led to a significant number of these "montes blancos" becoming "montes de propios" ('town council-owned lands'. Regarding the second identification, it can be inferred from the analysis of the Catalogue of Woodland of Public Utility of the province of Saragossa that, historically, many - but not all- "montes blancos" were deforested. Finally, the article analyses the origin and evolution of the "baldíos and realengos", lands which, while initially being part of common property, instead of evolving towards communal property, remained for centuries in a state of almost total non-definition as regards their ownership, as a res nullius, until the Civil Confiscation of 1855, which entailed either their sale (because they were considered "bienes de propios", their inclusion in State-owned property or their inclusion in municipal property (theoretically as communal property or 'property of common use': "bienes de aprovechamiento común", at which time this typology ceased to exist as a legal category.

    [es] El artículo analiza el significado de tres denominaciones de montes públicos muy comunes en Aragón y otros lugares de España, correspondientes a legislaciones periclitadas o

  8. Growth, mortality and migratory pattern of white shrimp (Litopenaeus vannamei, Crustacea, Penaeidae in the Carretas-Pereyra coastal lagoon system, Mexico

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    G Rivera-Velázquez

    2008-06-01

    Full Text Available The growth, mortality and migration pattern of the population of Litopenaeus vannamei Boone 1931 in the Carretas-Pereyra coastal lagoon system, Mexico, were studied. The shrimp spatial distribution and abundance were analyzed in relation to salinity, water temperature, and substrate. A total of 2 669 shrimps was collected at 22 sites sampled monthly from March 2004 to August 2005. Juvenile shrimps of L. vannamei were present in the coastal lagoon system throughout the year, reaching densities from 0.001 to 0.302 ind/m². The estimated daily growth rate was 0.06 to 0.27 mm carapace length (CL. No significant seasonal differences were appreciated. Weekly total mortality (Z was between 0.04 and 0.34. Recruits, juveniles and sub-adults displayed a bimodal distributional pattern regulated by the prevailing conditions during the dry season. The peak abundance of juvenile stages occurred in December-January and March-May. The abundance presented an inverse correlation with salinity (r=-0.42; pSe estudió el crecimiento, la mortalidad y el patrón de migración del camarón Litopenaeus vannamei Boone 1931 en el sistema lagunar costero Carretas Pereyra, México. La distribución espacial y la abundancia fueron analizadas con relación a la salinidad, temperatura y substrato. De marzo de 2004 a agosto de 2005 se recolectó un total de 2669 camarones con un muestreo mensual en 22 sitios. Los jóvenes se hallaron todo el año en el sistema lagunar costero, con densidades entre 0.001 y 0.302 ind/m². La tasa de crecimiento diaria fue de 0.06 a 0.27 mm longitud del cefalotórax (CL y no se apreciaron diferencias significativas entre estaciones. La mortalidad total (Z semanal estuvo entre 0.04 y 0.34. Reclutas, jóvenes y subadultos presentan un patrón de distribución bimodal regulado por las condiciones prevalecientes durante la estación de estío. Los valores máximos de abundancia de los estadios juveniles se presentan en diciembre-enero y marzo-mayo. La

  9. Influência do aquecimento sobre diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei (Boone, 1931 = Influence of heating under different titration methods of residual SO2 on shrimps Litopenaeus vannamei (Boone, 1931

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    Karla Patrícia Brito de Araújo Vieira

    2008-01-01

    Full Text Available O metabissulfito de sódio é o conservante mais aplicado na carcinicultura, com uso amparado em legislações vigentes no país, todavia, seu resíduo, dióxido de enxofre (SO2, em concentrações altas, pode provocar reações adversas à saúde. Este estudo foidesenvolvido com a finalidade de observar a influência do aquecimento sob diferentes métodos de titulação de SO2 residual em camarões Litopenaeus vannamei. Os camarões foram submetidos a nove concentrações de metabissulfito de sódio e avaliados com diferentesmétodos de detecção de SO2. O método da titulação Adolfo Lutz adaptado apresentou grande sensibilidade para detecção de SO2 residual. O método da titulação iodométrica com aquecimento possibilitou a detecção de maiores níveis de SO2, quando comparado aométodo sem o aquecimento. O aquecimento interferiu negativamente no método da fita reativa. Conclui-se que o aquecimento influenciou positivamente a titulação iodométrica, não ocorrendo o mesmo com a fita reativa.Sodium metabisulfite is the most applied preservative in shrimp culture, with its use supported by current legislation; however, its residue, sulfur dioxide (SO2, in high concentrations can provoke adverse health reactions. This study evaluated the influence of heating under different methods of residual SO2 titration in Litopenaeus vannamei. Shrimps were treated with nine concentrations of sodium metabisulfite and evaluated by different methods of SO2 titration. The adapted Adolfo Lutz titration method presented the highest sensitivity for the detection of SO2. The method of iodometric titration with heating made possible the detention ofhigher levels of SO2, when compared to the same method without heating. The heating influenced negatively on the reactive ribbon method. It is concluded that the heating influenced positively on the iodometric titration, as opposed to the reactive ribbon.

  10. Comportamento de Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae em função da oferta do alimento artificial nas fases clara e escura do período de 24 horas Behavior of Litopenaeus vannamei (Boone (Crustacea, Decapoda, Penaeidae in relation to artificial food offer along light and dark phases in a 24 h period

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    Cibele S. Pontes

    2005-09-01

    Full Text Available A escassez de dados acerca do comportamento do camarão marinho Litopenaeus vannamei (Boone, 1931 relacionado ao alimento artificial ofertado em comedouros poderá induzir a uma alimentação inadequada, aumentando a relação custo/benefício e os impactos ambientais potenciais do seu cultivo. Objetivando fornecer subsídios para a melhoria do manejo alimentar praticado nas fazendas, foram desenvolvidos estudos comportamentais utilizando 64 juvenis de L. vannamei (7,57 ± 1,01 g, submetidos a fotoperíodo artificial, metade deles em ciclo invertido, para observação das suas atividades durante as fases clara e escura. A ração foi oferecida em intervalos pré-estabelecidos, registrando-se antes e depois da oferta: exploração, natação, alimentação e inatividade (focal instantâneo. A natação ocorreu preferencialmente durante a fase escura. A alimentação foi mais elevada na primeira meia hora posterior à oferta, com ênfase nos horários da fase clara. O alimento artificial atuou, em ambas as fases, como um indutor da exploração do substrato, tornando os camarões mais ativos em todos os horários posteriores à sua oferta.The few data on the marine shrimp Litopenaeus vannamei (Boone, 1931 behavior related to artificial food offer in feeding trays may result an inadequate feeding by the animal, increasing the cost/benefit relation in shrimp culture and its potential environmental impact. In order to provide tools for optimizing feeding management in shrimp farms, a behavioral study was developed, using 64 L. vannamei juveniles (7,57 ± 1,01 g. They were submitted to artificial photoperiods, half of them in reversed cycle, in order to register their behavior during light and dark phases. In established intervals, the following activities were registered before and after food exposition: exploration, swimming, feeding and inactivity (instantaneous sampling. Swimming occurred mostly during the dark phase. Feeding time was higher in

  11. Efeito da adição do melaço na relação carbono/nitrogênio no cultivo de camarão Litopenaeus vannamei na fase berçário = Effect of molasses addition on carbon/nitrogen ratio in the nursery phase of Litopenaeus vannamei shrimp culture

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    Ugo Lima Silva

    2009-10-01

    Full Text Available Investigou-se o efeito da adição do melaço nas relações carbono:nitrogênio (C:N sobre o desempenho zootécnico do camarão Litopenaeus vannamei, na fase berçário, quando cultivado sem renovação de água. As relações do C:N foram avaliadas nas proporções de 25:1 (25M, 15:1 (15M e o controle (0M, sem aplicação de carbono, em delineamento experimental inteiramente casualizado, com quatro repetições. Pós-larvas (PL com peso inicial de 2,5 ± 0,5 mg, foram estocadas em 12 tanques (800 L volume útil, em densidades de 6,25 PL L-1, durante 42 dias de cultivo. Ao final do cultivo, os pesos finais dos camarões dos tratamentos 25M (532,0 mg e 15M (540,0 mg foram superiores (p The effect of molasses addiction on carbon:nitrogen ratios (C:N on the performance of Litopenaeus vannamei shrimp during the nursery phase cultured without water exchange was investigated. The C:N ratios were evaluated in 25:1 (25M and 15:1 (15M proportion and a control (0M, with no carbon source addition, in a randomized experimental design with four replicates. Post-larvae (PL with initial weight of 2.5 ± 0.5 mg were stocked in 12 tanks (800 L net volume, at the density of 6.25 PL L-1 during 42 culture days. At the end of culture, the shrimps weights on 25M (532.0 mg and 15M (540.0 mg treatments were higher (p < 0.05 than the 0M (428.6 mg treatment. Shrimp survival was high in all treatments (77.9 to 90.0%, but without significant difference (p ≥ 0.05. Therefore, the 15 and 25:1 C:N ratios showed an increased growth performance of nursery phase L. vannamei post-larvae cultured without water exchange.

  12. Seleção de variáveis em modelos matemáticos dos parâmetros de cultivo do camarão marinho Litopenaeus vannamei Selection of variables in mathematical models of culture parameters of marine shrimp Litopenaeus vannamei

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    Ady Marinho Bezerra

    2007-03-01

    Full Text Available O objetivo deste trabalho foi selecionar as variáveis de manejo do camarão marinho Litopenaeus vannamei que mais influenciaram nas variáveis-respostas ao cultivo (produção, produtividade, peso final e taxa de sobrevivência, em modelos matemáticos. O banco de dados foi composto por 83 cultivos, realizados no período de 2003 a 2005, obtidos de uma fazenda comercial localizada no litoral sul de Pernambuco. Para estimar os parâmetros dos modelos, utilizou-se a técnica dos mínimos quadrados. A seleção das variáveis foi realizada com o processo "backward elimination" associado ao método de transformação de Box e Cox. A adequação das equações e os pressupostos de normalidade e homocedasticidade, para os erros, foram analisadas com base na análise de variância e análise de resíduo. É possível relacionar essas variáveis e estabelecer predições com as equações.The objective of this work was to select management variables of the marine shrimp Litopenaeus vannamei that most influenced culture variable responses (production, productivity, final weight and survival rate, in mathematical models. The database was composed of 83 cultures in the period of 2003 to 2005, obtained from a shrimp farm located in the South coast of Pernambuco. To estimate the parameters of the models it was used the technique of least square. The selection of variable was carried through the backward elimination process associated to the Box and Cox transformation. The adequacy of the equations and the hypothesis of normality and homogeneous variance for the errors were analyzed based on the analysis of variance and on the analysis of residuals. It is possible to correlate those variables and to establish predictions with the equations.

  13. Effects of endosulfan exposure and Taura Syndrome Virus infection on the survival and molting of the marine penaeid shrimp, Litopenaeus vannamei.

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    Tumburu, Laxminath; Shepard, Eleanor F; Strand, Allan E; Browdy, Craig L

    2012-03-01

    Molting in crustaceans is an important endocrine-controlled biological process that plays a critical role in growth and reproduction. Many factors can affect this physiological cycle in crustaceans including environmental stressors and disease agents. For example the pathology of Taura Syndrome Virus (TSV) of shrimp is closely related to molting cycle. Similarly, endosulfan, a commonly used pesticide is a potential endocrine disruptor. This study explores interrelationships between pesticide exposure, virus infection and their interactions with physiology and susceptibility of the shrimp. Litopenaeus vannamei (Pacific white shrimp) were challenged with increasing doses of endosulfan and TSV (TSV-C, a Belize reference strain) to determine the respective median lethal concentrations (LC(50)s). The 96-h endosulfan LC(50) was 5.32 μg L(-1), while the 7-d TSV LC(50) was 54.74 mg L(-1). Subsequently, based on their respective LC(50) values, a 20-d interaction experiment with sublethal concentrations of endosulfan (2 μg L(-1)) and TSV (30 mg L(-1)) confirmed a significant interaction (pshrimp. Concurrently, molt-stage of animals, both at the time of exposure and death, was compared with mortality. For animals challenged with TSV, no strong correlation between molt-stage and mortality was observed (p>0.05). For animals exposed to endosulfan, animals in the postmolt stage were shown to be more susceptible to acute toxicity (p<0.05). For animals exposed to both TSV and endosulfan, interference of endosulfan-associated stress lead to increasingly higher susceptibility at postmolt (p<0.05) during the acute phase of the TSV disease cycle. Copyright © 2011 Elsevier Ltd. All rights reserved.

  14. Transcriptomic information from Pacific white shrimp (Litopenaeus vannamei) ovary and eyestalk, and expression patterns for genes putatively involved in the reproductive process.

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    Ventura-López, Claudia; Galindo-Torres, Pavel E; Arcos, Fabiola G; Galindo-Sánchez, Clara; Racotta, Ilie S; Escobedo-Fregoso, Cristina; Llera-Herrera, Raúl; Ibarra, Ana M

    2017-05-15

    The increased use of massive sequencing technologies has enabled the identification of several genes known to be involved in different mechanisms associated with reproduction that so far have only been studied in vertebrates and other model invertebrate species. In order to further investigate the genes involved in Litopenaeus vannamei reproduction, cDNA and SSH libraries derived from female eyestalk and gonad were produced, allowing the identification of expressed sequences tags (ESTs) that potentially have a role in the regulation of gonadal maturation. In the present study, different transcripts involved in reproduction were identified and a number of them were characterized as full-length. These transcripts were evaluated in males and females in order to establish their tissue expression profiles during developmental stages (juvenile, subadult and adult), and in the case of females, their possible association with gonad maturation was assessed through expression analysis of vitellogenin. The results indicated that the expression of vitellogenin receptor (vtgr) and minichromosome maintenance (mcm) family members in the female gonad suggest an important role during previtellogenesis. Additionally, the expression profiles of genes such as famet, igfbp and gpcr in brain tissues suggest an interaction between the insulin/insulin-like growth factor signaling pathway (IIS) and methyl farnesoate (MF) biosynthesis for control of reproduction. Furthermore, the specific expression pattern of farnesoic acid O-methyltransferase suggests that final synthesis of MF is carried out in different target tissues, where it is regulated by esterase enzymes under a tissue-specific hormonal control. Finally, the presence of a vertebrate type steroid receptor in hepatopancreas and intestine besides being highly expressed in female gonads, suggest a role of that receptor during sexual maturation. Copyright © 2016 Elsevier Inc. All rights reserved.

  15. Optimization of thermophysical properties of Pacific white shrimp (Litopenaeus vannamei) previously treated with freezing-point regulators using response surface methodology.

    Science.gov (United States)

    Wang, Liang; Liu, Zunying; Zhao, Yuanhui; Dong, Shiyuan; Zeng, Mingyong; Yang, Huicheng

    2015-08-01

    Three freezing-point regulators (glycine, sodium chloride and D-sorbitol) were employed to optimize thermophysical properties of Pacific white shrimp (Litopenaeus vannamei) using response surface methodology (RSM). The independent variables were glycine content (0.250-1.250 %), sodium chloride content (0.500-2.500 %) and D-sorbitol content (0.125-0.625 %) and analysis of variance showed that the effects of glycine, sodium chloride and D-sorbitol on the thermophysical properties were statistically significant (P freezing point (T i ), unfreezable water mass fraction (W u ), apparent specific heat (C app ) and Enthalpy (H) were 0.896 ~ 0.999. The combined effects of these independent variables on T i , W u , C app and H were investigated. The results indicated that T i , C app and H varied curvilinearly with increasing of glycine, sodium chloride and D-sorbitol content whereas W u increased nearly linearly. Based on response plots and desirability functions, the optimum combination of process variables for Pacific white shrimp previously treated with freezing-point regulators were 0.876 % for glycine content, 2.298 % for sodium chloride content and 0.589 % for D-sorbitol content, correspondently the optimized thermophysical properties were T i , - 5.086 °C; W u , 17.222 %; C app , 41.038 J/g °C and H, 155.942 J/g, respectively. Briefly, the application of freezing-point regulators depressed T i and obtained the optimum W u , C app and H, which would be obviously beneficial for the exploitation of various thermal processing and food storage.

  16. Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Camacho-Jiménez, Laura; Sánchez-Castrejón, Edna; Ponce-Rivas, Elizabeth; Muñoz-Márquez, Ma Enriqueta; Aguilar, Manuel B; Re, Ana Denisse; Díaz, Fernando

    2015-09-01

    Crustacean hyperglycemic hormone (CHH) is the most abundant neuropeptide produced by the X-organ/sinus gland (XO/SG) complex in the crustacean eyestalk. CHH plays a principal role in the control of glucose metabolism. The CHH-B1 isoform is produced in the eyestalk of Litopenaeus vannamei by alternative splicing of the chhB gene and its cDNA sequence has revealed that this isoform has a non-amidated C-terminal residue (CHH-like peptide). In this work, a recombinant CHH-B1 (rCHH-B1) with a sequence identical to the native hormone was expressed in the methylotrophic yeast Pichia pastoris X-33 and purified from the culture medium by RP-HPLC. The identity of the purified rCHH-B1 was confirmed by N-terminal sequencing and by using an anti-CHH-B1 polyclonal antibody. An in vivo assay showed that the hyperglycemic effect was dependant of the dosage of rCHH-B1, and the maximal hyperglycemic response was obtained with 250pmol treatment. These results suggest that the amino acid sequence of the C-terminus and its correct structure are both important for the hyperglycemic activity of naturally occurring non-amidated CHH peptides, such as CHH-B1. CHH-B1 appears to be the first reported CHH-like peptide with significant hyperglycemic activity produced in the sinus gland of a penaeid shrimp. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Use of Probiotic Bacillus spp. in Rotifer (Brachionus plicatilis) and Artemia (Artemia urmiana) Enrichment: Effects on Growth and Survival of Pacific White Shrimp, Litopenaeus vannamei, Larvae.

    Science.gov (United States)

    Jamali, Hadi; Imani, Ahmad; Abdollahi, Daruosh; Roozbehfar, Reza; Isari, Amin

    2015-06-01

    This study was to evaluate the effect of a preparation of Bacillus probiotic (Bacillus licheniformis and B. subtilis, 1:1) on growth and survival rate of Pacific white shrimp, Litopenaeus vannamei larvae. The larvae were fed on Artemia urmiana nauplii and Brachionus plicatilis enriched with the probiotic preparation at 1 × 10(6) CFU mL(-1) rate. The experimental setup was completely randomized design comprised of six treatments, namely solo Artemia nauplii (A) or rotifer (R), Artemia nauplii and rotifer without any enrichment (A + R), Artemia nauplii enrichment with probiotic bacilli (Bacillus licheniformis and B. subtilis) (A + B), rotifer enrichment with probiotic bacilli (R + B) and enriched Artemia nauplii and rotifer (A + R + B). All treatments were performed in triplicate. Chemical parameters of rearing water viz. pH, salinity and temperature were 7.5-8, 30-31 ppt and 31-32 °C, respectively. Photoperiod was 16L:8D. Shrimp larvae were fed Artemia nauplii and rotifers at 5-20 and 10-40 individuals per shrimp larvae four times a day, respectively. Growth and survival rate of larvae were determined at MII, MIII, PL1, PL4, PL7 and PL10 stages. Larvae in A + R + B treatment showed the highest total length (10.89 ± 0.51 mm), weight (674 ± 73 μg) and survival rate (65% ± 3.5). Lowest total length, weight and survival rate (7.96 ± 0.63 mm, 493 ± 52 μg and 24.5 ± 2.4%, respectively) were recorded in treatment B larvae. We concluded that Bacillus probiotic can improve growth and survival rate of Pacific white shrimp larvae without conceivably undesirable effects.

  18. Validation of a commercial insulated isothermal PCR-based POCKIT test for rapid and easy detection of white spot syndrome virus infection in Litopenaeus vannamei.

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    Yun-Long Tsai

    Full Text Available Timely pond-side detection of white spot syndrome virus (WSSV plays a critical role in the implementation of bio-security measures to help minimize economic losses caused by white spot syndrome disease, an important threat to shrimp aquaculture industry worldwide. A portable device, namely POCKIT™, became available recently to complete fluorescent probe-based insulated isothermal PCR (iiPCR, and automatic data detection and interpretation within one hour. Taking advantage of this platform, the IQ Plus™ WSSV Kit with POCKIT system was established to allow simple and easy WSSV detection for on-site users. The assay was first evaluated for its analytical sensitivity and specificity performance. The 95% limit of detection (LOD of the assay was 17 copies of WSSV genomic DNA per reaction (95% confidence interval [CI], 13 to 24 copies per reaction. The established assay has detection sensitivity similar to that of OIE-registered IQ2000™ WSSV Detection and Protection System with serial dilutions of WSSV-positive Litopenaeus vannamei DNA. No cross-reaction signals were generated from infectious hypodermal and haematopoietic necrosis virus (IHHNV, monodon baculovirus (MBV, and hepatopancreatic parvovirus (HPV positive samples. Accuracy analysis using 700 L. vannamei of known WSSV infection status shows that the established assayhassensitivity93.5% (95% CI: 90.61-95.56% and specificity 97% (95% CI: 94.31-98.50%. Furthermore, no discrepancy was found between the two assays when 100 random L. vannamei samples were tested in parallel. Finally, excellent correlation was observed among test results of three batches of reagents with 64 samples analyzed in three different laboratories. Working in a portable device, IQ Plus™ WSSV Kit with POCKIT system allows reliable, sensitive and specific on-site detection of WSSV in L. vannamei.

  19. TOTAL REPLACEMENT OF FISHMEAL WITH AN ORGANICALLY CERTIFIED YEAST–BASED PROTEIN IN PACIFIC WHITE SHRIMP (Litopenaeus Vannamei DIETS: LABORATORY AND FIELD TRIALS

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    Ewen McLean

    2006-04-01

    Full Text Available The feasibility of totally replacing the fishmeal component of marine shrimp (Litopenaeus vannamei diets was examined both in the laboratory setting and during a full–scale commercial trial. Animals were fed either a traditional fishmeal–based diet or one in which complete replacement of fishmeal, on a per protein basis, was manufactured using a yeast–based product, NuPro®. Laboratory studies determined that irrespective of diet fed, no difference in shrimp performance (weight gain, survival and SGR occurred. A field trial was thus activated to determine whether lab–scale studies were transferable to the commercial setting. Trials were conducted in earthen ponds from mid–June to early November 2005. Ponds were initially stocked with PL12–16 shrimp at a rate of 100,000 per hectare. At trial end, ponds receiving the NuPro®–based feed had equivalent growth to that of shrimp fed the traditional, fishmeal–based diet. Percent increase in weight from initial values and survival for the NuPro® ponds was 296, 269 and 275%, and 78, 76 and 85% respectively, whereas that for the fishmeal–based diet was 305% and 80% respectively. Noteworthy was that within pond size variation of L. vannamei was lower in NuPro® fed animals (±2.3 g when compared against animals receiving the traditional feed (±4.1 g. Overall observations from the field trial indicate the importance of the »bioreactor« pond with respect to the supply of energy to sustain shrimp growth potential.

  20. Pacific white shrimp (Litopenaeus vannamei) vitellogenesis-inhibiting hormone (VIH) is predominantly expressed in the brain and negatively regulates hepatopancreatic vitellogenin (VTG) gene expression.

    Science.gov (United States)

    Chen, Ting; Zhang, Lv-Ping; Wong, Nai-Kei; Zhong, Ming; Ren, Chun-Hua; Hu, Chao-Qun

    2014-03-01

    Ovarian maturation in crustaceans is temporally orchestrated by two processes: oogenesis and vitellogenesis. The peptide hormone vitellogenesis-inhibiting hormone (VIH), by far the most potent negative regulator of crustacean reproduction known, critically modulates crustacean ovarian maturation by suppressing vitellogenin (VTG) synthesis. In this study, cDNA encoding VIH was cloned from the eyestalk of Pacific white shrimp, Litopenaeus vannamei, a highly significant commercial culture species. Phylogenetic analysis suggests that L. vannamei VIH (lvVIH) can be classified as a member of the type II crustacean hyperglycemic hormone family. Northern blot and RT-PCR results reveal that both the brain and eyestalk were the major sources for lvVIH mRNA expression. In in vitro experiments on primary culture of shrimp hepatopancreatic cells, it was confirmed that some endogenous inhibitory factors existed in L. vannamei hemolymph, brain, and eyestalk that suppressed hepatopancreatic VTG gene expression. Purified recombinant lvVIH protein was effective in inhibiting VTG mRNA expression in both in vitro primary hepatopancreatic cell culture and in vivo injection experiments. Injection of recombinant VIH could also reverse ovarian growth induced by eyestalk ablation. Furthermore, unilateral eyestalk ablation reduced the mRNA level of lvVIH in the brain but not in the remaining contralateral eyestalk. Our study, as a whole, provides new insights on VIH regulation of shrimp reproduction: 1) the brain and eyestalk are both important sites of VIH expression and therefore possible coregulators of hepatopancreatic VTG mRNA expression and 2) eyestalk ablation could increase hepatopancreatic VTG expression by transcriptionally abolishing eyestalk-derived VIH and diminishing brain-derived VIH.

  1. Application of YHV-protease dsRNA for protection and therapeutic treatment against yellow head virus infection in Litopenaeus vannamei.

    Science.gov (United States)

    Assavalapsakul, Wanchai; Chinnirunvong, Wanlop; Panyim, Sakol

    2009-04-06

    While farming of the Pacific white shrimp Litopenaeus vannamei is well established in North and South America, the industry has more recently been introduced to Asia, and the Pacific white shrimp is now the most commonly farmed species in Thailand. However, outbreaks of yellow head virus (YHV) disease in the Pacific white shrimp have caused severe economic losses and currently there is no effective prevention or treatment of YHV infections. The YHV-protease double-stranded RNA (YHV-Pro dsRNA) can act as both a prophylactic agent and as a treatment to inhibit YHV replication in infected black tiger shrimp Penaeus monodon. The utility of this methodology to other shrimp species has not, however, been established. The purpose of this study was to determine whether YHV-Pro dsRNA can be applied to the Pacific white shrimp. To assess prophylactic efficiency, YHV-Pro dsRNA was injected into juvenile shrimp 24 h prior to challenge with YHV. Subsequent YHV replication was inhibited by YHV-Pro dsRNA as compared with injection of an unrelated dsRNA. For therapeutic treatment of YHV-infected shrimp, shrimp were challenged with YHV before dsRNA injection. Injection of YHV-Pro dsRNA up to 6 h post-infection resulted in the almost complete elimination of YHV replication. These results suggest that YHV-Pro dsRNA can also be broadly applied as a prophylactic agent to inhibit YHV replication and therapeutic treatment of YHV-infected Pacific white shrimp.

  2. Effects of Microencapsulated Synbiotic Administration at Different Dosages against heavy co-infection of White Spot Disease (WSD and Vibrio harveyi in Pacific White Shrimp (Litopenaeus vannamei

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    Yunarty Yunarty

    2016-12-01

    Full Text Available White spot disease (WSD is one of infectious disease in shrimp caused by white spot syndrome virus (WSSV. This study aimed to determine the dosage immunological effects and growth performances of microencapsulated synbiotic (Bacillus NP5 and mannan oligosaccharide at different dosages on Pacific white shrimp.  The microencapsulated synbiotic   was administered as feed supplementation  against the co-infection of   WSSV and Vibrio harveyi. Synbiotic was encapsulated by spray drying method, further feed supplemented to Pacific white shrimp for 30 days at a  dosages of 0.5% (A, 1% (B, 2% (C and control treatments, i.e. without any microencapsulated synbiotic administration as positive control (D and negative control (E. The challenge test was performed on day 30 after feeding supplementation, then the experimental shrimps were injected by WSSV intramuscularly   at the infective dosage of 104 copies.-ml-1. Afterwards,   24 hours after WSSV injection the shrimps were immersed in water contained cells suspension of V. harveyi  at the cells population dosage of 106 CFU-.ml-1. All synbiotic treatments showed better results with the values of Total Haemocyte Count (THC, Phenoloxidase (PO and Respiratory Burst (RB, were higher (P<0.05 compared to positive control. The specific growth rates (SGR of A, B and C showed higher than both controls of D and E. The feed conversion ratio (FCR value of synbiotic treatments were lower (P<0.05 than both controls. However, the administration of microencapsulated synbiotic have not been able to prevent heavy impact of WSSV and V. harveyi co-infection due to lower SR and mortality pattern which continued to increase.   Keywords: Synbiotic, Litopenaeus vannamei, WSSV, Vibrio harveyi, co-infection

  3. Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei.

    Science.gov (United States)

    Adel, Milad; El-Sayed, Abdel-Fattah M; Yeganeh, Sakineh; Dadar, Maryam; Giri, Sib Sankar

    2017-06-01

    The aims of this study were to evaluate the effects of Lactococcus lactis subsp. lactis on the growth, intestinal microbiota, digestive enzyme activity, and disease resistance of Litopenaeus vannamei. Diets containing four different concentrations of L. lactis (0 [basal diet], 10 6 , 10 7 , and 10 8  CFU g -1 ) were fed to white shrimps L. vannamei (average weight 5.89 ± 0.36 g) for 8 weeks. At the end of the feeding trial, shrimps were immersed in Caspian Seawater (10.8 ppt) contaminated with 10 6  CFU ml -1 pathogenic V. anguillarum for 2 h. Results revealed that growth rate, survival, and body protein level were increased with dietary supplementation of L. lactis. The activities of digestive enzymes (cellulose, lipase, amylase, and protease) were significantly higher in the groups fed with diets containing 10 7 or 10 8  CFU g -1 L. lactis than those in the control. The Lactobacillus and Bacillus counts were higher (P lactis-supplemented diets. In addition, higher level of L. lactis supplementation decreased the Vibrio counts. Moreover, L. vannamei fed diet supplemented with 10 8  CFU g -1 of L. lactis exhibited significantly the highest hematocyte count and post-challenge survival rate (79.2 %). Collectively, these results suggest that dietary supplementation of L. lactis subsp. lactis at 10 8  CFU g -1 can promote growth performance, digestive enzyme activity, and disease resistance of L. vannamei.

  4. Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Chen, Ting; Ren, Chunhua; Wang, Yanhong; Luo, Peng; Jiang, Xiao; Huang, Wen; Chen, Chang; Hu, Chaoqun

    2016-07-01

    The full-length cDNA coding for a novel invertebrate (i-type) lysozyme was identified in Pacific white shrimp (Litopenaeus vannamei). The newly obtained L. vannamei lysozyme is similar to the Penaeus monodon i-type lysozyme 2, but it is distant from the known L. vannamei c-type lysozyme and i-type lysozyme 1 in protein sequence; therefore, it was defined as L. vannamei i-type lysozyme 2 (lyz-i2). Expression of L. vannamei lyz-i2 transcripts were ubiquitously detected in all tissues we selected, with the highest abundance observed in the hemolymph. Challenge with Vibrio harveyi might elicit L. vannamei lyz-i2 mRNA expression in the hepatopancreas, intestine, muscle, gill and hemolymph. In the themolymph, specifically, the stimulatory effects of Vibrio and lipopolysaccharide (LPS) on lyz-i2 transcript levels were durable and transient, respectively; while Polyinosinic:polycytidylic acid [Poly (I:C)] treatment did not affect lyz-i2 expression. L. vannamei lyz-i2 recombinant protein was generated in an Escherichia coli system. By lysoplate and turbidimetric assays, the L. vannamei lyz-i2 recombinant protein showed a broad spectrum of antimicrobial properties with high activities against Micrococcaceae lysodeikticus and various Vibrio species and relatively low activity against E. coli. In conclusion, L. vannamei lyz-i2 might be a potent antibacterial protein with a role in innate immunity in Penaeid shrimp. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Modificaciones en la grasa del atún blanco (Thunnus alalunga debidas a la fabricación y almacenamiento de su conserva

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    Navarro, María P.

    1991-06-01

    Full Text Available Modifications in fatty acid composition in white tuna (Thunnus alalunga ocurring at each stage of the cannig process, boiling and sterilization in soybean oil, were studied. Boiling was performed in brine and two sterilization times (55 and 90 minutes as well as the effects of storage for one year were tested. Boiling had no effect on fatty acid composition or the n-3/n-6 ratio. However canned tuna absorbed soybean oil during sterilization, causing an increase of the percentage of C18:1, C18:2 and C18:3 and a decrease in C20:5 and C22:6. Therefore, unsaturated fatty acids to saturated fatty acids (PS/S ratio changed and the n-3/n-6 ratio declined from 7.03 to 0.59 compared with raw tuna. Due to the whole fat enrichment, canned fish showed a slight loss of PUFA (C20:5 and C22:6 and a increase of C18:1, C18:2 and C18:3. The latter effect was more marked in the preserves as a consequence of one year storage. The convenience of using for cannig an oil containing a lower amount of polyunsaturated fatty acid in order to remain the original n-3/n-6 ratio of the raw tuna is discussed.Se estudiaron los cambios que se producen en la grasa del atún blanco en cada una de las etapas que intervienen en el proceso de fabricación y almacenamiento de su conserva: cocción en salmuera y esterilización practicada en aceite de soja. Se estudiaron dos tiempos diferentes de esterilización (55 y 90 minutos y el almacenamiento por espacio de 1 año. La cocción en salmuera no afectó a la composición en ácidos grasos de los lípidos del pescado ni a la relación n-3/n-6. Sin embargo, durante la esterilización, el atún absorbió el aceite utilizado como cobertura, lo que produjo un aumento porcentual en el nivel de C18:1, C18:2, C18:3 y una disminución en C20:5 y C22:6. La relación ácidos grasos insaturados a saturados (PS/S cambió y la n-3/n-6 disminuyó desde 7,03 a 0,59 comparado con atún crudo. En el pescado en conserva, debido al enriquecimiento total

  6. Influências da Guerra Fria no discurso nacionalista argentino. O retrato dos conflictos internacionais no Semanário Nacionalista Azul y Blanco

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    Maria Valeria Galvan

    2014-12-01

    Full Text Available As notícias sobre as guerras internacionais desencadeadas no marco da Guerra Fria têm sido utilizadas pela discursividade nacionalista em pós do seu programa político próprio, mais vinculado ao contexto local do que ao internacional.O semanário político dos anos cinqüenta e sessenta, Azul y Blanco, virou testemunha disso. Este, embora tenha-se concentrado principalmente na política nacional, deixou filtrar em suas páginas notícias internacionais que expunham as problemáticas mais importantes da Guerra Fria nesses anos, desde uma óptica nacionalista que ia mais longe das identidades nacionais para construir um “nós” geograficamente mais amplo. Assim, apesar da maior relevância da Revolução Cubana nas páginas da revista, a situação do bloco oriental europeu e a Guerra do Vietnã foram também importantes na secção internacional. Essas notícias eram escritas por correspondentes e editores argentinos e estrangeiros. Ambos argentinos e estrangeiros tiveram posicionamentos políticos e ideológicos que ficaram comprometidos com seus respectivos cenários locais. Assim, eles contribuíram especialmente para as transformações que atravessava o discurso nacionalista local. Nesse sentido, as redefinições ideológicas e políticas do nacionalismo argentino desses anhos nutriram-se também de aqueles olhares.

  7. Insectos asociados a fenómenos de descomposición cadavérica en cerdo blanco ( Sus scrofa en el municipio de Mosquera (Cundinamarca

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    María Fernanda Ospina Fonseca

    2002-07-01

    Full Text Available El estudio tuvo como objeto iniciar la investigación de los insectos asociados a fenómenos dedescomposición en una zona alta, específicamente en el municipio de Mosquera (Cundina-marca. Para lograrlo se usó como biomodelo cerdo blanco (Sus scrofa por su similitud conlos humanos, éste se sacrificó y se dejó al aire libre durante ocho meses. El proceso de des-composición se dividió en cinco fases: fresco (2 días, hinchado (13 días, descomposiciónactiva (15 días, descomposición avanzada (31 días y restos secos (160 días. Durante esteperiodo se recogieron 3.539 individuos, el 86.69% pertenecientes a Diptera y el 8.36% aColeoptera. Las familias más numerosas de Diptera fueron Muscidae (62.08% y Calliphoridae(14.44%, mientras que en Coleoptera fueron Silphidae (68.58% y Staphylinidae (14.86%.Se pudo observar la participación de los géneros Calliphora, Paralucilia y Chloroprocta, estosgéneros no aparecen en zonas bajas y podrían ser propios de zonas altas. No se presentarongrupos exclusivos para cada fase del proceso, aunque la presencia y abundancia de Diptera fuemayor al inicio de la descomposición, mientras que Coleoptera (exceptuando Silphidae fuemás abundante y activo al final del proceso. La lluvia ocasionó una "regresión" en la sucesiónde insectos. El presente trabajo no constituye una herramienta de carácter legal pero es elprimer trabajo sobre entomología forense realizado en la Sabana de Bogotá y es base paratrabajos posteriores en la zona.

  8. Petrografía y distribución de óxidos de Fe, Al y Si en fragipanes blancos de origen volcánico

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    Enrique Ortiz-Hernández

    2012-10-01

    Full Text Available El objetivo fue determinar la petrología y distribución de óxidos de Fe, Al y Si en tres perfiles de suelos de origen volcánico con horizontes endurecidos (fragipán, en la región noroeste del Estado de México. Se utilizaron técnicas químicas selectivas para extraer Fe, Al y Si. El fragipán blanco clasifica petrográficamente como toba dacítica poco soldada y alterada. Los perfiles muestran incipiente a moderado grado de alteración de minerales primarios que contienen Fe y Al dadas las condiciones climáticas de la región. Los contenidos de alofano, ferrihidrita, y óxidos de hierro cristalino se presentan en pocas cantidades, escasos contenidos de Ald y Si lábil en los perfiles indican presencia de aluminosilicatos cristalinos. Bajas relaciones de Alp/Alo y Fep/Feo evidencia que la mayor parte de Al y Fe están unidos a compuestos inorgánicos pobremente cristalinos. Relaciones bajas de Fep/Fed y Alp/Ald indican limitada asociación de Fe y Al con compuestos orgánicos. La relación arcilla/Fed revela emigración de arcilla y óxidos de Fe del horizonte superior al fragipán.  El Si, Al y Fe en el fragipán forman parte de la estructura de los silicatos y mínimo grado de cristalinidad de Fe y Al pedogenético. Valores mayores de relación Sid(Sid+Ald en el fragipán indican aumento en la dureza.

  9. AVALIAÇÃO DO GANHO DE PESO DE PÓS-LARVAS DO CAMARÃO MARINHO Litopenaeus vannamei (BOONE, 1931, ALIMENTADOS COM PEIXES DA FAUNA ACOMPANHANTE DO CAMARÃO MARINHO EVALUATION OF WEIGHT GAIN IN POST-LARVAE OF MARINE SHRIMP Litopenaeus vannamei (BOONE, 1931 FED ON FISH SPECIES WHICH COMPOSE THE MARINE SHRIMP FISHERIES BYCATCH

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    Henrique José Mascarenhas dos Santos Costa

    2007-04-01

    Full Text Available O objetivo do presente trabalho foi analisar o desenvolvimento inicial de pós-larvas de Litopenaeus vannamei em água doce utilizando como alimento peixes da fauna acompanhante da pesca do camarão marinho. Cultivaram-se 48 pós-larvas com peso e comprimento médio total iniciais de 0,008±0,001 g e 11,0±0,5 mm, respectivamente. Para cada tratamento foram utilizados, como alimento, ração comercial (RC para camarão marinho com 45% de proteína bruta, Opisthonema oglinum (OO (sardinha bandeira e Chloroscombus chrysurus (CC (palombeta, respectivamente. Utilizou-se o delineamento de blocos ao acaso com quatro repetições. A densidade de estocagem consistiu de quatro camarões por aquário. Durante os 75 dias de experimento, a alimentação foi administrada ad libitum. Registrou-se a temperatura média final de 27,1±0,8ºC para os três tratamentos e o pH médio final de 7,68±0,27 para os camarões alimentados com RC e 7,66±0,22 para os indivíduos alimentados com OO e CC, respectivamente. Os pesos médios finais foram de 0,560±0,096 g, 0,495±0,091 g e 0,500±0,101 g e os comprimentos totais finais de 62,1±0,9 mm, 57,0±0,8 mm e 56,2±0,9 mm para os tratamentos com RC, OO e CC, respectivamente. Os resultados do peso e comprimento final mostraram que não houve diferença estatística significativa entre os tratamentos testados. A sobrevivência final foi de 87,5±14,4%, 68,8±12,5% e 62,5±14,3% para o RC, OO e CC, respectivamente. Conclui-se que a utilização de ração para camarão marinho e os peixes marinhos O. oglinum e C. chrysurus como alimento para pós-larvas de camarão marinho apresentam desenvolvimento semelhante. PALAVRAS-CHAVE: Camarão marinho, dietas, Chloroscombus chrysurus, Litopenaeus vannamei, Opisthonema oglinum. The objective of this study was to evaluate the early development of Litopenaeus vannamei post-larvae in freshwater fed on fish species that compose the shrimp fisheries bycatch. Forty eight post

  10. Aplicação de ácido giberélico (GA3 em précolheita de tangerina ‘Poncã’ (Citrus reticulata blanco = Application of Gibberelic acid (GA3 on preharvest of ‘Ponkan’ mandarin (Citrus reticulata Blanco fruit

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    Júnior Cesar Modesto

    2006-01-01

    Full Text Available O experimento foi instalado em pomar comercial de tangerineira ‘Poncã’ (Citrus reticulata Blanco, enxertadas sobre tangerineira ‘Cleópatra’ (Citrus reshni Hort. ex Tan., em Pratânia, Estado de S��o Paulo, Brasil. Adotouse o delineamento experimental em blocoscasualizados com 4 repetições. Os tratamentos empregados foram: 0 (controle, 5, 10, 15 e 20 mg L1 de ácido giberélico (GA3. A aplicação dos tratamentos foi realizada com atomizador tratorizado. Na mudança de coloração dos frutos, utilizaramse 5,9 litros de solução por planta, com adição de 0,03% de surfatante não iônico com 25% de Alquil fenol poliglicoléter. As análises da qualidade dos frutos foram realizadas aos 13, 45, 75 e 111 dias após a aplicação dos tratamentos (DAT. Não se verificou influência da aplicação de GA3 na massa fresca dos frutos, no conteúdo de sólidos solúveis totais, na acidez total titulável e “ratio”. No entanto foi observado atraso na colheita de frutos, induzido pelo efeito fisiológico do ácido giberélico. The experiment was carried out in a commercial orchard of ‘Ponkan’ mandarin (Citrus reticulata Blanco, grafted on ‘Cleopatra’ mandarin (Citrus reshni Hort. ex Tan., in Pratânia, state of São Paulo, Brazil. The utilized experimental design was composed of randomized blocks of four replications. The treatments consisted of: 0 (control,5, 10, 15 and 20 mg L1 of Gibberellic acid (GA3. Atomizer equipment was utilized for the application of the treatments. Each plant received 5.9 liters of solution, in which 0.03% of nonionic surfactant with 25% of alkylphenol ethoxylate was added, in order to change fruit coloration. The analyses of fruit quality were developed on the 13th, 45th, 75th and 111th days after the application of the treatments (DAT. Results showed that the application of GA3 did not affect fruit fresh mass, total soluble solids content, total titulable acidity and ratio. However, a delay in fruit

  11. Hábitos alimenticios y migratorios del tiburón blanco Carcharodon carcharias (Lamniformes: Lamnidae de Isla Guadalupe inferidos por el análisis de isótopos estables de δ15N and δ13C

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    Mario Jaime-Rivera

    2014-08-01

    Full Text Available La composición isotópica de los tejidos de los depredadores tope en el ambiente marino provee información sobre su ecología trófica y su comportamiento migratorio. Estudios previos han mostrado que el tejido dérmico puede registrar patrones largos de movimiento y caza. El objetivo de este estudio fue describir los hábitos tróficos y migratorios de los tiburones blancos de Isla Guadalupe realizando un análisis de isótopos estables de su tejido dérmico. Consideramos un pequeño grupo de muchos posibles taxa que los tiburones pudieron haber comido a lo largo de su migración: pinípedos, calamares y atunes. Estas presas fueron agrupadas en cinco áreas focales: Golfo de California, Isla Guadalupe, Costa de California, SOFA y Hawái. Realizamos un modelo de mezcla bayesiano para estudiar la ecología trófica de este depredador tope. Los promedios del valor isotópico de la dermis del tiburón blanco fueron δ13C (-14.5‰ y δ15N (19.1‰. Los promedios del valor isotópico de la dermis transformada para semejar músculo fueron δ13C (-16.6 ‰ y δ15N (21.2‰. El modelo de mezcla mostró una probable depredación de los tiburones en áreas oceánicas como el SOFA y confirmó la importancia de los pinnípedos como presa principal del tiburón blanco en Isla Guadalupe.

  12. Actividad antioxidante del té blanco y de los residuos de limón: optimización de la extracción y aplicaciones en carne y en envases activos

    OpenAIRE

    Peiró Sánchez, Sara

    2015-01-01

    [spa] Este proyecto de investigación estudia la extracción, optimización de la extracción y uso de los polifenoles naturales procedentes de los residuos de limón (Lemon Waste, LW) y de té blanco (White Tea, WT), el té más antioxidante del mundo. Persigue un objetivo doble, encontrar y testar nuevas fuentes de productos y compuestos naturales bioactivos que puedan sustituir o disminuir el uso de compuestos sintéticos, y reutilizar y revalorizar residuos agroalimentarios. En la primera etapa d...

  13. Las hormonas sexuales como moduladoras de la función mitocondrial y de la síntesis de adiponectina en el tejido adiposo blanco y en el músculo esquelético de rata

    OpenAIRE

    Capllonch Amer, Gabriela

    2014-01-01

    El objetivo principal de esta tesis es caracterizar los efectos de las hormonas sexuales sobre la función mitocondrial del tejido adiposo blanco (TAB) y del músculo, y su relación con la síntesis de adiponectina. Nuestros resultados establecen el 17beta-estradiol como estimulador de la funcionalidad mitocondrial y de la síntesis de adiponectina en adipocitos y músculo, mientras que la testosterona actúa como un elemento negativo sobre los mismos procesos en los adipocitos. Asimismo, se ha est...

  14. Diseño y construcción del prototipo de un sistema electrónico por ultrasonido para medir distancias aplicada a un bastón blanco

    OpenAIRE

    Ayala Cruz, Edy Leonardo

    2011-01-01

    La presente es una investigación que evalúa el uso de un sistema para la detección de obstáculos que impiden la movilidad "capacidad para desplazarse de un lugar a otro de forma independiente, segura y eficaz" (Mstr. Miryam Gallegos Navas. Manual para maestría en educación especialidad con mención en educación en de las personas con discapacidad visual. Universidad Politécnica Salesiana. Pág. 43. 2007) de los No Videntes que utilizan como herramienta el Bastón Blanco. Se diseñó un disposit...

  15. El factor de crecimiento transformante beta como blanco terapéutico Transforming growth factor-beta as a therapeutic target

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    Francisco Javier Gálvez-Gastélum

    2004-08-01

    Full Text Available El factor de crecimiento transformante beta (TGF-beta es una familia de proteínas que incluye al TGF-beta, activinas y a la proteína morfogénica de hueso (BMP, por sus siglas en inglés, citocinas que son secretadas y se relacionan estructuralmente en diferentes especies de metazoarios. Los miembros de la familia del TGF-beta regulan diferentes funciones celulares como proliferación, apoptosis, diferenciación, migración, y tienen un papel clave en el desarrollo del organismo. El TGF-beta está implicado en varias patologías humanas, incluyendo desórdenes autoinmunes y vasculares, así como enfermedades fibróticas y cáncer. La activación del receptor del TGF-beta propicia su fosforilación en residuos de serina/treonina y dispara la fosforilación de proteínas efectoras intracelulares (smad, que una vez activas se translocan al núcleo para inducir la transcripción de genes blanco, y así regular procesos y funciones celulares. Se están desarrollando novedosas estrategias terapéuticas encaminadas a corregir las alteraciones presentes en patologías que involucran al TGF-beta como actor principal.Transforming growth factor-beta (TGF-beta family members include TGF-beta, activins, and bone morphogenetic proteins (BMP. These proteins are structurally related cytokines secreted in diverse Metazoans. TGF-beta family members regulate cellular functions such as proliferation, apoptosis, differentiation, and migration, and play an important role in organism development. Deregulated TGF-beta family signaling participates in various human pathologies including auto-immune diseases, vascular disorders, fibrotic disease, and cancer. Ligand-induced activation of TGF-beta family receptors with intrinsic serine/threonine kinase activity, triggers phosphorylation of the intracellular effectors of TGF-beta signaling, the Smads proteins. Once these proteins are activated they translocate into the nucleus, where they induce transcription of target

  16. Salud pública e inestabilidad política en Venezuela durante los gobiernos de Guzmán Blanco, 1870-1888

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    Camacho, Cristian

    2008-12-01

    Full Text Available In this paper, we analyze how during Guzmán Blanco governmental periods epidemic cycles generated a sense of distrust and political instability. The misinterpretation form governmental authorities lead to think that oppositionists promoted the closure of ports, a halt on exchange and limitations to the access to economic resources coming from foreign trade with the purpose of obtain political benefits. To avoid oppositionists politically use epidemics cycles, governmental action were addressed to prevent rumors and to put down any political action related to this matter. Gúzman government also efficiently carried out an information policy from consular offices aimed to take advantage from up-to-date information with respect to vessels movements and their internal health situation. Moreover, a set of highly effective national and international preventative measure were adopted in order to control and manage epidemic cycles.

    En este artículo se analiza cómo durante el guzmancismo, los ciclos epidémicos ocasionaban en el gobierno un ambiente de desconfianza y tensión política, porque de manera equivocada se pensaba que eran empleados por algunos opositores para incidir en el cierre de los puertos, paralizar el intercambio, cortar el flujo de recursos económicos provenientes del comercio exterior y obtener así beneficios políticos. El gobierno se movilizaba para evitar rumores y reprimir cualquier tipo de acción política al respecto, con el fin de impedir que la oposición diera uso político a los ciclos epidémicos; también ejecutaba con eficiencia una política de información que, desde los Consulados, le permitía estar al corriente acerca del movimiento de los barcos y su situación sanitaria interna; además se desarrolló un conjunto de previsiones nacionales e internacionales altamente benéficas en el control y manejo de los períodos epidémicos.

  17. Efecto del tratamiento térmico de desestabilización en el desgaste de hierros blancos de alto cromo

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    Iván Morales

    2009-11-01

    Full Text Available La resistencia a la abrasión de hierros fundidos de alto cromo puede ser mejorada mediante la aplicación de tratamientos térmicos específicos que favorezcan tanto la transformación de austenita en martensita, como la precipitación de carburos secundarios. En esta investigación se estudió, mediante microscopia óptica, el efecto de la temperatura de desestabilización entre 900oC y 1050oC de una aleación 26Cr2.5C2Mo de hierro blanco. La resistencia a la abrasión fue medida en términos de la pérdida de masa durante un ensayo de desgaste abrasivo según la norma ASTM G65. La resistencia a la abrasión de las muestras mejoró al ser tratada térmicamente mediante un proceso de desestabilización a una temperatura determinada, para lo cual se optimizó el tamaño de los carburos secundarios y la dureza superficial. / The heat treatments used to improve abrasion resistance of High-Chromium white casted irons (WCI tend to ease the retained austenite/martensite transformation temperature and favor carbide precipitation within the metallic matrix. In this paper, the effect of four different destabilization heat treatment temperatures was examined for a 26Cr2.5C2Mo WCI under means of optical microscopy. The specimens were obtained under two different casting process, regular sand-casting and permanent molding. The abrasion resistance was measured as mass loss in a dry sand/rubber wheel abrasion test according to ASTM G65. The results show that the solidification speed has a direct effect on the abrasion resistance; manly due to both the eutectic carbide size and its distribution. As predicted, the abrasion resistance of both samples was maximized under certain destabilization temperature, for which carbide size and hardness was optimized.

  18. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the MONTE OLIVIA and Rio Blanco in the English Channel, North Atlantic Ocean and others from 2009-01-18 to 2009-12-23 (NODC Accession 0117337)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117337 includes Surface underway, chemical, meteorological and physical data collected from MONTE OLIVIA and Rio Blanco in the English Channel, North...

  19. Effects of the probiotic, Bacillus subtilis E20, on the survival, development, stress tolerance, and immune status of white shrimp, Litopenaeus vannamei larvae.

    Science.gov (United States)

    Liu, Kuan-Fu; Chiu, Chiu-Hsia; Shiu, Ya-Li; Cheng, Winton; Liu, Chun-Hung

    2010-01-01

    In this study, the probiotic, Bacillus subtilis E20, isolated from the human health food, natto, was used for white shrimp, Litopenaeus vannamei, larvae breeding to improve the larval survival rate and development by adding probiotic to the rearing water at (control), 10(8), and 10(9) cfu L(-1) salt water once every 3 days during the 14 days of breeding experiment. Thereafter, stress tolerance and immune status of postlarvae were evaluated. Shrimp larval development was significantly accelerated after adding the probiotic to the larval rearing water at a level of 10(9) cfu L(-1). The survival rate of larvae was significantly higher in the treatment with 10(9) cfu L(-1) compared to the control and the treatment with 10(8) cfu L(-1) after all larvae had metamorphosed to postlarvae. Adding the probiotic to the shrimp larvae rearing water produced a weak inhibition of bacterial growth by an analysis of the total bacterial count and presumptive Vibrio count. For stress tests, no postlarvae died when they were reared in water in which the temperature was decreased from 30 to 2 degrees C at a rate of 0.1 degrees C min(-1). Postlarvae had significantly lower cumulate mortality in the treatments with 10(8) and 10(9) cfu L(-1) compared to the control when they were suddenly exposed to fresh water and 60 per thousand salt water. A significant decrease in the cumulative mortality of postlarvae treated with the probiotic at a level of 10(9) cfu L(-1) was recorded after the sudden transfer to 300 mg L(-1) nitrite-N compared to the control and treatment with 10(8) cfu L(-1). The analysis of immune-related gene expressions showed that the gene expression of prophenoloxidase I, prophenoloxidase II, and lysozyme of larvae were significantly increased after being reared in probiotic-containing water at the levels of 10(8) and 10(9) cfu L(-1). However, no significant difference in serine proteinase or glutathione peroxidase gene expressions was recorded in this study. It is therefore

  20. Modulation of innate immunity and gene expressions in white shrimp Litopenaeus vannamei following long-term starvation and re-feeding.

    Science.gov (United States)

    Lin, Yong-Chin; Chen, Jiann-Chu; C Man, Siti Nursafura; W Morni, Wan Zabidii; N A Suhaili, Awangku Shahrir; Cheng, Sha-Yen; Hsu, Chih-Hung

    2012-01-01

    The survival rate, weight loss, immune parameters, resistance against Vibrio alginolyticus and white-spot syndrome virus (WSSV), and expressions of lipopolysaccharide- and ß-glucan-binding protein (LGBP), peroxinectin (PX), prophenoloxidase-activating enzyme (ppA), prophenoloxidase (proPO) I, proPO II, α2-macroglobulin (α2-M), integrin ß, heat shock protein 70 (HSP70), cytosolic manganese superoxide dismutase (cytMnSOD), mitochondrial manganese superoxide dismutase (mtMnSOD), and extracellular copper and zinc superoxide dismutase (ecCuZnSOD) were examined in the white shrimp Litopenaeus vannamei (8.18 ± 0.86 g body weight) which had been denied food (starved) for up to 14-28 days. Among shrimp which had been starved for 7, 14, 21, and 28 days, 100%, 90%, 71%, and 59% survived, and they lost 3.2%, 7.3%, 9.2%, and 10.4% of their body weight, respectively. Hyaline cells (HCs), granular cells (GCs, including semi-granular cells), the total haemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), and SOD activity significantly decreased in shrimp which had been starved for 1, 1, 1, 5, 14, and 3 days, respectively. The expression of integrin ß significantly decreased after 0.5-5 days of starvation, whereas the expressions of LGBP, PX, proPO I, proPO II, ppA, and α2-M increased after 0.5-1 days. Transcripts of all genes except ecCuZnSOD decreased to the lowest level after 5 days, and tended to background values after 7 and 14 days. Cumulative mortality rates of 7-day-starved shrimp challenged with V. alginolyticus and WSSV were significantly higher than those of challenged control-shrimp for 1-7 and 1-4 days, respectively. In another experiment, immune parameters of shrimp which had been starved for 7 and 14 days and then received normal feeding (at 5% of their body weight daily) were examined after 3, 6, and 12 h, and 1, 3, and 5 days. All immune parameters of 7-day-starved shrimp were able to return to their baseline values after 5 days

  1. Estimation of genetic parameters and genotype-by-environment interactions related to acute ammonia stress in Pacific white shrimp (Litopenaeus vannamei) juveniles at two different salinity levels.

    Science.gov (United States)

    Lu, Xia; Luan, Sheng; Cao, Baoxiang; Meng, Xianhong; Sui, Juan; Dai, Ping; Luo, Kun; Shi, Xiaoli; Hao, Dengchun; Han, Guomin; Kong, Jie

    2017-01-01

    Regarding the practical farming of Litopenaeus vannamei, the deterioration of water quality from intensive culture systems and environmental pollution is a common but troublesome problem in the cultivation of this species. The toxicities that result from deteriorating water quality, such as that from ammonia stress, have lethal effects on juvenile shrimp and can increase their susceptibility to pathogens. The toxicity of ammonia plays an important role in the frequently high mortality during the early stage on shrimp farms. However, little information is available regarding the genetic parameters of the ammonia tolerance of juveniles in the early stage, but this information is necessary to understand the potential for the genetic improvement of this trait. Considering the euryhalinity of L. vannamei and the fact that low salinity can increase the toxicity of ammonia stress, we estimated the heritability of ammonia tolerance in juveniles in 30‰ (normal) and 5‰ (low) salinity in this study using the survival time (ST) at individual level and the survival status at the half-lethal time (SS50) at the family level. In the normal and low salinity conditions and for the merged data, the heritability estimates of the ST (0.784±0.070, 0.575±0.068, and 0.517±0.058, respectively) and SS50 (0.402±0.061, 0.216±0.050, and 0.264±0.050, respectively) were all significantly greater than zero, which indicates that the ammonia-tolerance of shrimp can be greatly improved. So it might provide an alternative method to reduce mortality, help to enhance resistance to pathogens and reduce the occurrence of infectious diseases. The significant positive genetic correlation between ST and body length suggested that ammonia is more toxic to shrimp in the early stage. The medium-strength genetic correlations of the ST and SS50 between the two environments (0.394±0.097 and 0.377±0.098, respectively) indicate a strong genotype-by-environment (G×E) interaction for ammonia tolerance

  2. [Apparent digestion coefficients for dry matter, protein and essential amino acids in terrestrial ingredients for Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)].

    Science.gov (United States)

    Terrazas, Martín; Civera, Roberto; Ibarra, Lilia; Goytortúa, Ernesto

    2010-12-01

    Protein quality mainly depends on the essential amino acid (EAA) profile, but also on its bioavailability, because EAA digestibility is generally lower than the analyzed amounts. This information is needed in the aquaculture industry for aquafeed formulation. For this purpose, the apparent digestibility coefficients of dry matter, protein, and essential amino acids of eight feedstuffs of terrestrial origin were determined for the juvenile whiteleg shrimp Litopenaeus vannamei (15-19 g), using 1% chromic oxide as an inert marker. A reference diet was formulated and produced in the laboratory. Eight experimental diets were prepared each with 30% of one of the experimental ingredients added to the reference diet: casein, porcine byproduct meal poultry byproduct meal, corn meal, wheat gluten meal, soybean paste, sorghum meal, and wheat meal. The experiment consisted of a single-factor, completely randomized design with three replicates per treatment. Samples of ingredients, diets and feces were analyzed for nitrogen and amino acids. For amino acid assay, we used reverse-phase high performance liquid chromatography. To avoid partial loss of methionine and cystine, samples of ingredients, diets, and feces were oxidized with performic acid to methionine sulfone and cysteic acid prior to acid hydrolysis. The apparent dry matter and protein digestive utilization coefficients varied from 68% to 109% and from 70% to 103%, respectively. Apparent digestibility of protein for casein, soy paste, wheat meal and wheat gluten were very high (over 90%), corn gluten and poultry byproducts meal showed high protein digestibility (over 80%), but porcine byproducts meal and sorghum meal had low digestibility (76% and 70%, respectively). There was a reasonable, but not total, correspondence between apparent protein digestibility and average essential amino acid digestibility coefficients, except for arginine in corn gluten, phenylalanine and leucine in sorghum meal, phenylalanine in soy

  3. Influência de duas dietas na qualidade da água dos tanquesberçário, utilizados no cultivo do camarão marinho Litopenaeus vannamei (Boone, 1931

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    Adriano Prysthon da Silva

    2006-01-01

    Full Text Available Cultivos experimentais com pós-larvas do Litopenaeus vannamei, na fase de berçário, foram realizados em uma fazenda de camarão, objetivando avaliar a influência da utilização de duas dietas na qualidade físico-química das águas residuais. As dietas foram formuladas à base de ração comercial e náuplios de artêmia, denominadas MAC e MAA, respectivamente. Foram utilizadas pós-larvas no estágio (PL19, estocadas a 16PL/L em dois tanques-berçário de 60 m3. Ao final de 10 dias de cultivo, as concentrações dos compostosnitrogenados (amônia, nitrito e nitrato e fosfatados (fósforo total e ortofosfatos dos tanquesberçário foram menores na dieta MAA do que na MAC (pExperiments were carried out in a commercial marine shrimp farm in order to evaluate the use of two diets in Litopenaeus vannamei post-larvae, during nursery phase, on physiochemical wastewater quality. Two feeding methods were adopted: Commercial Feeding Method (MAC and Artemia Feeding Method (MAA. The nursery tanks (60 m3 were provided with 19 days post-larvae (PL19 with a stocking density of 16PL/liter. After 10 days of culture, the concentrations of nitrogen (ammonia, nitrite and nitrate and phosphorus compounds (total phosphorus and orthophosphate in the nursery tank were smaller for MAC, rather than MAA treatment (p<0.05. Consequently, MAA treatment induced low levels of daily compounds increment,thus contributing to a significant reduction of the nutrients that caused water eutrophication.

  4. The impact of oxygen consumption by the shrimp Litopenaeus vannamei according to body weight, temperature, salinity and stocking density on pond aeration: a simulation = Impacto do consumo de oxigênio do camarão Litopenaeus vannamei em relação ao peso corporal, temperatura, salinidade na aeração do viveiro: uma simulação

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    Luis Vinatea

    2011-04-01

    Full Text Available A simulation was conducted to determinate the impact caused by thecombination of Litopenaeus vannamei respiratory rate (mg O2 shrimp-1 h-1, the behavior of SOTR (kg O2 h-1 of mechanical aerators as a function of salinity, as well as the oxygen consumption rate of the pond water and soil (mg O2 L-1 h-1 on the aeration of shrimp ponds (1, 10, 50 and 100 ha stocked with different densities (10, 40 and 120 shrimp m-2, salinities (1, 13, 25 and 37 ppt, temperatures (20, 25 and 30°C, and shrimp wet weight (5, 10, 15 and 20 g. Results showed that under lower salinity, with larger shrimp, and higher stocking density, higher will be the quantity of required 2-HP aerators to keep dissolved oxygen over 50% saturation. In addition, under low salinity, with 5 and 10 g shrimp, independent of stocking density, more aerators per hectare are required and electricity cost is higher at 20°C and salinity 1 ppt. Less aerators and lower electricity cost was observed at 30°C, salinities of 25 and 37 ppt, and shrimp of 15 and 20 g. Baseado em estudos de respiração de Litopenaeus vannamei (mg O2 camarão-1 h-1, comportamento do Standard Oxygen Transfer Rate (SOTR, kg O2 h-1 de aeradores mecânicos em função da salinidade, assim como as taxas de respiração da água e do solo (mg O2 L-1 h-1, uma simulação foi realizada a fim de determinar o impacto que estas três variáveis juntas têm sobre a aeração de viveiros (1, 10, 50 e 100 ha, estocados com diferentes densidades (10, 40 e 120 camarões m-2 em salinidades de 1, 13, 25 e 37 ppm, temperaturas de 20, 25 e 30°C e peso úmido dos camarões de 5, 10, 15 e 20 g. Os resultados mostraram que em salinidades mais baixas, com animais maiores e maiores densidades de estocagem, maior será a quantidade de aeradores de 2 cvnecessários para manter o oxigênio dissolvido acima de 50% da saturação. Igualmente, em baixas salinidades e com camarões de 5 e 10 g, independente da densidade de estocagem, mais aeradores

  5. Phytoplanktonic composition of three cultivation systems used in Litopenaeus vannamei (BOONE, 1931 marine shrimp farms = Composição fitoplanctônica em três sistemas de cultivo do camarão marinho Litopenaeus vannamei (BOONE, 1931

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    Michelle Pereira Melo

    2010-07-01

    Full Text Available The aim of this work is to assess the different compositions of phytoplankton in three cultivation systems of marine shrimps Litopenaeus vannamei (BOONE, 1931, denominated as organic, intensive and semi intensive. The samples were done fortnightly, when phytoplankton was collected by a net for phytoplankton, 65 ƒÊm mesh, being then filtrated in a total volume of water of 100 L, and preserved in formaldehyde solution at 4% and identified according to the methodology of Cordeiro et al. (1997. The results show that the densities of Diatoms were of 16.65, 10.47 and 7.57 cel. 103 mL-1 for the organic, intensive and semi intensive cultivations, respectively. As for cyanobacteria, the average figures were 42.06 cel. 103 mL-1 forsemi intensive 17.27 cel. 103 mL-1, in the intensive cultivation and 6.11 cel. 103 mL-1 for the organic cultivation system. The dinoflagellates had the highest cellular density in the phytoplankton community analyzed with 61.9 cel. 103 mL-1 in the intensive cultivation, 0.33 and 0.03 cel. 103 mL-1 for both semi intensive and organic cultivation systems respectively. Euglenas presented the results of 4.98 and 14.86 cel. 103 mL-1 only for semi intensive and intensive cultivations. It was then concluded that all cultivations presented average rates below recommended for such studied systems.Conduziu-se esse trabalho com o objetivo de avaliar as diferentes composicoes fitoplanctonicas em tres sistemas de cultivo para o camarao marinho Litopenaeus vannamei (BOONE, 1931, denominados de organico, intensivo e semiintensivo. As amostragens foram realizadas quinzenalmente, onde o fitoplancton foi coletado atraves de uma rede de plancton, com malha de 65 ƒÊm, sendo filtrado um volume de agua total de 100 litros, que foram preservadas em solucao de formol a 4% e identificadas segundo a metodologia de Cordeiro et al. (1997. Os resultados mostram que as densidades de diatomaceas foram de 16,65; 10,47 e 7,57 cel. 103 mL-1, respectivamente

  6. Efeito do tipo de sedimento na eficiência alimentar, crescimento e sobrevivência de Litopenaeus vannamei (Boone, 1931 - doi: 10.4025/actascibiolsci.v33i4.6134 Effect of sediment type on feed efficiency, growth and survival of Litopenaeus vannamei (Boone, 1931 - doi: 10.4025/actascibiolsci.v33i4.6134

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    Fúlvio Aurélio Morais Freire

    2011-09-01

    Full Text Available Este estudo avaliou o desempenho zootécnico do camarão marinho Litopenaeus vannamei em diferentes tipos de substratos não consolidados. Juvenis (0,97 ± 0,27 g foram aclimatados na densidade de 52 m-2, em 21 caixas de polietileno com biofiltros individuais, em um sistema fechado de filtração contínua, sendo submetidos aos tratamentos: 1 A = 25% silte + 25% argila + 25% areia muito fina + 25% areia fina; 2 B = 50% areia fina + 50% areia muito fina e 3 Controle = sem substrato, em um delineamento experimental completamente casualizado, com três tratamentos e sete repetições cada. Os animais foram alimentados com 12% da biomassa/dia com ração peletizada (35% de proteína bruta, ofertada em bandejas e parcelada em duas ofertas diárias (8 e 16h. O estudo teve duração de 48 dias, sendo avaliados: consumo aparente de ração, eficiência alimentar, taxa de crescimento específico, sobrevivência e ganho de peso. Foram acompanhados diariamente salinidade, pH, amônia e temperatura da água. A análise estatística dos dados se deu por meio dos testes Anova ou Kuskal-Wallis, em função da sua parametricidade. Não foram observadas diferenças significativas com relação aos fatores avaliados nas diferentes granulometrias de sedimento testadas, indicando que estas não exerceram influência sobre o desempenho zootécnico de L. vannamei juvenil.This study aimed to evaluate the zootechnical performance of Litopenaeus vannamei shrimp at different types of unconsolidated substrate. Juvenile shrimp (0.97 ± 0.27 g were acclimatized, in a stocking density of 52 m-2, to 21 polyethylene boxes (50 L connected individually to biofilters in a closed water exchange system. The animals were submitted to the treatments: 1 A = 25% silt + 25% clay + 25% very fine sand + 25% fine sand; 2 B = 50% fine sand + 50% very fine sand and 3 Control = no sediment, with seven replications each. Photoperiod was set on 12L: 12D. Shrimps were fed twice a day (at 08

  7. Genetics of two marine shrimp hatcheries of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931 in Pernambuco, Brazil Genética de duas larviculturas de camarão branco do Pacífico Litopenaeus vannamei (Boone, 1931 em Pernambuco, Brasil

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    Ana Patrícia Souza de Lima

    2010-02-01

    Full Text Available The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution. Since then, broodstock replacement in hatcheries has been based on domestic stocks, raising concerns on the decline of genetic diversity and if the existing diversity would allow effective genetic improvement programs. In the present research, genetic parameters such as number of alleles, effective allele number, expected and observed heterozygosities, inbreeding coefficient, genetic differentiation index and deviation from Hardy-Weinberg equilibrium have estimated of two important commercial hatcheries in Northeast Brazil, genotyping 5 microsatellite loci. Effective allele number (3 to 10.5 and average observed and expected heterozygosities (0.480 and 0.680 were consistent with those reported for cultured and wild Penaeid populations. However, F IS positive values (0.381 for hatchery A and 0.249 for hatchery B reflected a significant heterozygous deficiency within hatcheries (PA carcinicultura cresceu significativamente no Brasil ao longo dos últimos 10 anos, especialmente a produção do camarão branco do Pacífico, o exótico Litopenaeus vannamei. Em 2004, a atividade foi marcada por uma crise na produção, que despertou interesse na implantação de programas de melhoramento dos estoques de camarão. A importação de crustáceos foi banida do Brasil por uma Instrução Normativa de 1997, como uma medida de precaução sanitária. Desde então, a reposição de matrizes nas larviculturas passou a ser conduzida com estoques domesticados, gerando preocupações sobre o possível declínio da diversidade genética e sobre a

  8. Influência de duas dietas na qualidade da água dos tanquesberçário, utilizados no cultivo do camarão marinho Litopenaeus vannamei - DOI: 10.4025/actascianimsci.v28i1.670 The influence of two diets on the quality of water in nursery tanks used in marine shrimp (Litopenaeus vannamei, Boone, 1931 - DOI: 10.4025/actascianimsci.v28i1.670

    Directory of Open Access Journals (Sweden)

    Paulo de Paula Mendes

    2006-01-01

    Full Text Available Cultivos experimentais com pós-larvas do Litopenaeus vannamei, na fase de berçário, foram realizados em uma fazenda de camarão, objetivando avaliar a influência da utilização de duas dietas na qualidade físico-química das águas residuais. As dietas foram formuladas à base de ração comercial e náuplios de artêmia, denominadas MAC e MAA, respectivamente. Foram utilizadas pós-larvas no estágio (PL19, estocadas a 16PL/L em dois tanques-berçário de 60 m3. Ao final de 10 dias de cultivo, as concentrações dos compostos nitrogenados (amônia, nitrito e nitrato e fosfatados (fósforo total e ortofosfatos dos tanquesberçário foram menores na dieta MAA do que na MAC (p Experiments were carried out in a commercial marine shrimp farm in order to evaluate the use of two diets in Litopenaeus vannamei post-larvae, during nursery phase, on physiochemical wastewater quality. Two feeding methods were adopted: Commercial Feeding Method (MAC and Artemia Feeding Method (MAA.0 The nursery tanks (60 m3 were provided with 19 days post-larvae (PL19 with a stocking density of 16PL/liter. After 10 days of culture, the concentrations of nitrogen (ammonia, nitrite and nitrate and phosphorus compounds (total phosphorus and orthophosphate in the nursery tank were smaller for MAC, rather than MAA treatment (p < 0.05. Consequently, MAA treatment induced low levels of daily compounds increment, thus contributing to a significant reduction of the nutrients that caused water eutrophication.

  9. Calidad de vida en el trabajo: Profesionales de la salud de Clínica Río Blanco y Centro de Especialidades Médicas Quality of life at work: Health Professionals Clinica Rio Blanco and Center Especialidades Médicas

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    Diemen Delgado García

    2012-09-01

    Full Text Available Objetivo: Identificar un constructo sobre la calidad de vida en el trabajo de los médicos/as y enfermeros/as de Clínica Río Blanco y Centro de Especialidades Médicas. Métodos y Materiales: Estudio transversal y analítico. Se aplicó un cuestionario de datos socio-laborales integrados por 10 ítems y el instrumento CVT-GOHISALO, el cual consta de 74 ítems y mide la calidad de vida en el trabajo en siete dimensiones Resultados: En un grupo de 20 trabajadores entre médicos/as y enfermeros/as, el 40 % cuenta con dos trabajos, el 20 % reporta ausentismo por enfermedad el año previo. El 60 % se desempeñan principalmente en turno mixto. El 40 % tiene una antigüedad de dos a cinco años y el 30 % menos de dos años. El número de horas semanales que dedican al descanso es menor a 20 horas en un 50 % de los trabajadores. Las siete dimensiones evaluadas: soporte institucional para el trabajo, seguridad en el trabajo, integración al puesto de trabajo, satisfacción por el trabajo, bienestar a través del trabajo, desarrollo personal y administración del tiempo libre, reflejan grados de insatisfacción en la calidad de vida en el trabajo. Conclusiones: Los trabajadores de la salud evaluados muestran una serie de percepciones subjetivas que reflejan una realidad de su medio laboral.Objective: To identify a construct on quality of life in the work of doctors and nurses Clínica Río Blanco and Center Especialidades Médicas. Methods and Materials: Cross-sectional and analytical. A questionnaire was social and labor data consisting of 10 question and CVT-GOHISALO instrument, which consists of 74 question and measures the quality of life at work in seven dimensions. Results: In a group of 20 workers among doctors and nurses, 40 % have two jobs, 20 % reported absenteeism due to illness the previous year. 60 % work mainly in mixed shift. 40 % have a length of two to five years and 30 % less than two years. The number of hours per week devoted to rest

  10. Adaptación de Cultivares de Ajo Morado y Blanco (Allium sativum L. en Acatlán, Guerrero, México. Adaptación de Cultivares de Ajo Morado y Blanco (Allium sativum L. en Acatlán, Guerrero, México

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    Efrén Cevallos Ruiz

    2012-02-01

    Full Text Available En el estado de Guerrero, México, el ajo se cultiva en la región Centro, en los municipios de Chilapa y Zitlala, donde los productores acostumbran sembrar el genotipo criollo adaptado a las condiciones climáticas de la región. El objetivo fue estudiar la adaptación del cultivo del ajo de los tipos morado y blanco a la región Centro de Guerrero por su rendimiento y calidad, ya sea para el mercado nacional o el de exportación. La investigación se llevó a cabo durante el ciclo otoño-invierno 2000-2001 en la comunidad de Acatlán, municipio de Chilapa de Álvarez, Guerrero. Se evaluaron 13 materiales de ajo, bajo un diseño experimental de bloques completos al azar con cuatro repeticiones; se hizo la separación de medias por medio de la prueba de Tukey P In the state of Guerrero, Mexico, garlic is cultivated in the Central region, at Chilapa and Zitlala municipalities, where the growers cultivate Criollo Regional genotype adapted to the climatic conditions of the region. The object of this investigation was to study the adaptability of the cultivars of the purple and the white garlic in Guerrero’s central region, considering both their yield and quality for the domestic and/or export markets. Research work was conducted during the 2000- 2001 fall-winter cycle, in Acatlan, municipality of Chilapa de Alvarez, Guerrero. Thirteen garlic cultivars were evaluated using completely randomized blocks design, with four repetitions. Mean separation using Tukey test P

  11. Detección de fusarium verticillioides y fumonisinas en granos de maíz blanco provenientes de los estados Yaracuy y Guárico, Venezuela

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    Marleny Chavarri

    2017-01-01

    Full Text Available El maíz ( Zea mays L. es uno de los cereales con mayor superf icie sembrada a nivel mundial, debido a su importancia en la alimentación humana y animal. Son muchos los agentes etiológicos que ocasionan enfermedades en la plantas de maíz; sin embargo, F u sarium verticillioides ( Sacc. Nirenberg , es uno de los mohos a l os cuales se le ha prestado especial atención por su amplia distribución, especialmente en zonas tropicales y subtropic a les, su capacidad toxigénica y su resistencia. Por tal motivo, se evaluó la incidencia de F. vertic i llioides y el contenido de fumonisi nas en granos de maíz blanco de once híbridos destinados al consumo humano provenientes de los estados Guárico y Yaracuy, Venezu e la . Método : La cuantificación de especies toxigénicas se realizó por siembra directa de granos enteros y desinfectados con Na ClO al 3,27%, sobre el medio malta sal agar, expresando los resultados como porcentajes de granos colonizados por mohos totales y por especies pote n cialmente toxigénicas. El contenido de fumonisinas se cuantificó por el método inmunoqu í mico con columnas de inmunoafinidad para fumonisinas (B1+B2. Resultados : No se observaron diferencias estadísticas significativas para la incidencia de F. verticillioides y la concentración de fumonisinas en las muestras evaluadas en ambos est a dos. La micobiota asociada a los granos de maíz fueron Aspergillus flavus, Aspergillus N iger, A. ochraceus, A. terreus, Eurotium chevalieri, F. verticillioides, Penicillium spp. Altern a ria sp ., y Curvularia sp. La mayor inciden cia promedio de mohos toxigénico s l a obtuvi e ron A. flavus (24,3%, F. verticillioides (14,6% y Penicillium sp . (4,4% en Yaracuy; no obstante, en Gu á rico fueron F. verticillioides (16,7% y Penicillium spp. (13,8%. La concentración promedio de fumonisinas en Yaracuy y Guárico fue de 1,8 y 8,4μg/g, respectivamente, superando el límite permitido para consumo humano (1 μg/g. Discusi

  12. PROPAGACIÓN POR ESTACAS JUVENILES DEL BALSO BLANCO (Heliocarpus americanus L. Sin. H. popayanensis UTILIZANDO PROPAGADORES DE SUBIRRIGACIÓN PROPAGATION OF WHITE BALSUM (Heliocarpus americanus L. Sin. H. popayanensis CUTTINGS USING NONMIST PROPAGATORS

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    Carolina Vásquez Restrepo

    2006-12-01

    Full Text Available El mercado mundial de edulcorantes orgánicos es una oportunidad para la panela, producto básico de la canasta familiar que representa ingresos importantes para la población rural colombiana. El balso blanco (Heliocarpus americanus L. Sin. H. popayanensis Hook & Arn. es la especie más usada en procesos de clarificación de la panela en Antioquia y las zonas cañeras húmedas colombianas. Pero la extracción de la corteza de árboles obtenidos de la regeneración natural comienza a ser insostenible, por el daño causado a los individuos y la presión creciente a este recurso. La prohibición del uso de sustancias químicas en procesos de clarificación de panela ha aumentado la demanda de la corteza del balso blanco. En este trabajo se estimó el enraizamiento de estacas juveniles de balso blanco, por propagadores de subirrigación. Se realizaron dos experimentos, utilizando ácido anaftalenacético (ANA. En el primero se evaluó el efecto del transporte, cicatrizante y sustrato sobre el enraizamiento de las estacas de balso blanco. El mejor medio de transporte fue en cristales de hidrogel para mantener la humedad de las estacas, sin utilizar cicatrizante y sembrándolas en el sustrato tierra (55 % de enraizamiento. En el segundo se analizó la influencia de la intensidad lumínica y el área foliar en el porcentaje de enraizamiento de las estacas. El más alto enraizamiento se obtuvo con el tratamiento de doble sombra y un área foliar de 20 cm² (25 % de enraizamiento. Aunque los mejores resultados indican un relativo éxito en el uso de medios de enraizamiento, es una primera aproximación para propagar esta especie que necesita ser protegida.The world market of organic edulcorants is an opportunity for “panela”, a basic domestic consumption product that provides important income to rural population. White balsum (Heliocarpus americanus L. Sin. H. popayanensis Hook & Arn. is the most used species in the panela clarification processes in

  13. Analysis of the effects of Zimbabwean white farmers on small scale farming in Nigeria Análisis de los efectos de la presencia de agricultores blancos de Zimbabwe en la agricultura a pequeña escala en Nigeria

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    Matthew Olaniyi Adewumi

    2013-05-01

    migrantes extranjeros en los sistemas de agricultura a pequeña escala prevale­cientes en Nigeria. El Proyecto de Desarrollo de la Agricultura Zona D, en el cual los agricul­tores blancos se establecieron en el estado de Kwara, fue utilizado como caso de estudio. La información primaria fue obtenida de agricultores blancos así como de agricultores locales considerando su situación pre y post la llegada de los agricultores blancos. Las estadísticas descriptivas y el análisis del presupuesto de cada parcela fueron utilizados para evaluar la información. La mayoría de los agricultores locales (98.63% han transitado hacia el monocultivo desde la llegada de los agricultores blancos a la región. Se ha observado un incremento significativo en la tasa de uso de semillas, el uso de fertilizantes y otros agro­químicos, así como en el trabajo humano por agricultor en el área en comparación con la situación prevaleciente antes de la llegada de los agricultores blancos. El tamaño promedio de sus tierras, la distancia de estas con respecto de sus hogares y uso de tractores se ha reducido de forma importante, mientras que la producción por agricultor se ha incrementa­do. La búsqueda de políticas que tranquilicen a los agricultores locales quienes en ocasio­nes manifiestan su insatisfacción por la forma en que los agricultores blancos han llegado a establecerse en estas tierras resulta imperativa. Esto permitirá lograr un desarrollo positivo y sustentable. Estas políticas pueden ser desarrolladas a través de un enfoque participativo en el desarrollo de la agricultura en el área. Este enfoque también podría resultar relevante en otras regiones del mundo que presentan situaciones similares.

  14. Evaluación de Cultivares de Ajo Morado y Blanco por su Rendimiento Agronómico e Industrial en Irapuato, Guanajuato. Evaluación de Cultivares de Ajo Morado y Blanco por su Rendimiento Agronómico e Industrial en Irapuato, Guanajuato

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    José Luis Barrera Guerra

    2012-02-01

    Full Text Available Field yield, bulb quality, and industrial yield of garlic (Allium sativum L. cultivars were evaluated during the 1988-1989, 1989-1990, 1991-1992 and 2001-2002 fall-winter cycles at the Institute of Agricultural Sciences of the University of Guanajuato. Purple and white color bulb varieties were evaluated with variable results, depending on the genotype and vegetative cycle. None of the short life cycle cultivars qualified for field or industrial yield and bulb quality. Best field yields, higher weight per bulb and smaller clove number per bulb were obtained with: Cristal, Chileno Compuesto No. 1, and Taiwán. For industrial yield (t ha-1dehydrated garlic, the best cultivars were Cristal, Pocitas, Hermosillo, and Napuri. It is important to continue with the program of garlic genetic breeding, based on selection of new garlic lines derived from the best genotypes and using techniques as the virus elimination for improving yield and quality.Se evaluó el rendimiento, variables de calidad del bulbo y rendimiento industrial de cultivares de ajo (Allium sativum L., durante los ciclos otoño-invierno 1988-1989, 1989-1990, 1991-1992 y 2001-2002, en el Instituto de Ciencias Agrícolas de la Universidad de Guanajuato. Se emplearon genotipos de color morado y blanco, con resultados variables de acuerdo con su genotipo y ciclo vegetativo. Ninguno de los cultivares precoces calificó para rendimiento de campo, calidad o rendimiento industrial. Entre los cultivares de ciclo intermedio o tardío sobresalieron por su rendimiento en campo, mayor peso promedio de bulbo y menor número de dientes por bulbo los siguientes: Cristal, Chileno Compuesto No.1 y Taiwán. Para rendimiento industrial (t ha-1 de ajo deshidratado, sobresalieron los cultivares Cristal, Pocitas, Hermosillo y Nápuri. Es importante continuar con el programa de mejoramiento genético de ajo para la región de El Bajío, basado en selección de nuevas líneas de entre los genotipos mejores y con

  15. Experimental infection with different bacterial strains in larvae and juvenile Litopenaeus vannamei reared in Santa Catarina State, Brazil = Infecção experimental em larvas e juvenis de Litopaenaeus vannamei cultivados no Estado de Santa Catarina, Brasil

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    Celso Carlos Buglione

    2010-07-01

    Full Text Available This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and theeffects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrioalginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p Este estudo avaliou as características patogênicas de cepas de bactérias isoladas de Litopenaeus vannamei durante surto de mortalidade no Laboratório de Camarões Marinhos, UFSC, Estado de Santa Catarina, Brasil. Seu potencial de virulência em larvas e juvenis de camarão marinho e os efeitos sobre a contagem total de hemócito, atividade de fenoloxidase e título aglutinante do soro foramavaliados após infecção experimental. As cepas bacterianas foram isoladas de larvas e de camarões adultos e identificadas bioquimicamente pelo sistema API20E como: duas cepas de Vibrio alginolyticus, três de Aeromonas salmonicida e uma de Pasteurella sp. e P. multocida. Todas as cepas isoladas provocaram mortalidade em L. vannamei, e uma de V. alginolyticus resultou em mortalidade de 97,3 e 88,7% para larvas e juvenis de camarões, respectivamente. O sistema imunológico dos camarões juvenis sofreu influência da infecção experimental

  16. Molecular characterization and susceptibility to antimicrobial drugs of isolated bacterials from shrimps (“Litopenaeus vannamei” Caracterização molecular e susceptibilidade aos antimicrobianos de isolados bacterianos de camarões

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    Ricardo Castelo Branco Albinati

    2010-06-01

    Full Text Available The objective was to isolate bacteria from gut of shrimps from Litopenaeus vannamei, by biochemical characterization and molecular identification, inhibition activity in vitro of Bacillus cereus and sensitivity pattern determination. The bacterial species isolated were: Aeromonas caviae (n = 7, Alcaligenes denitrificans (n = 1, Bacillus cereus (n = 1 and Enterobacter spp. (n = 3. Bacillus cereus isolated in this study did not have inhibitory activity to other shrimps isolated bacteria evaluated. In the susceptibility to antimicrobial drug test, it were observed 68,7% to erythromycin, 50% to tetracycline, 81,2% to trimethoprim:sulfamethoxazole, neomycin and estreptomycin, 12,5% to lincomycin and ampicillin, 87,5% to enrofloxacin and nitrofurantoin, 93,7% to ceftriaxone, 100% to norfloxacin and nalidix acid. The characterization molecular is important on identifying the microrganisms studied. The nalidixic acid and norfloxacin are antimicrobial drugs with high sensitivity for bacteria isolated from shrimps.Objetivou-se isolar bactérias provenientes do trato intestinal de camarões da espécie Litopenaeus vannamei, por meio da caracterização bioquímica e molecular, atividade de inibição in vitro do Bacillus cereus e perfil de sensibilidade aos antimicrobianos. As espécies bacterianas identificadas foram Aeromonas caviae (n = 7, Alcaligenes denitrificans (n = 1, Bacillus cereus (n = 1 e Enterobacter spp. (n = 3. Bacillus cereus obtido neste estudo não apresentou atividade de inibição frente às demais bactérias isoladas de camarões. Quanto ao perfil de sensibilidade aos antimicrobianos, foram observados 68,7% de eritromicina, 50% de tetraciclina, 81,2% de sulfametoxazol/trimetoprina, neomicina e estreptomicina, 12,5% de lincomicina e ampicilina, 87,5% de enrofloxacina e nitrofurantoína, 93,7% de ceftriaxona, 100% de norfloxacina e ácido nalidíxico. A caracterização molecular é útil para identificação dos microrganismos estudados

  17. Alterações químicas, físicas e físico-químicas da tangerina 'ponkan' (Citrus reticulata Blanco durante o armazenamento refrigerado Chemical, physical and physical-chemical changes on tangerina 'ponkan' (Citrus reticulata Blanco under refrigerated storage

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    Adriana Aparecida Souza Vale

    2006-08-01

    Full Text Available O objetivo proposto neste trabalho foi o de avaliar as alterações químicas, físicas e físico-químicas da tangerina 'Ponkan' (citrus reticulata Blanco durante o armazenamento refrigerado a 5 ± 2° C e umidade relativa de 85 ± 3% por um período de 28 dias, em dois estádios de maturação (frutos coletados no início da safra; com a casca mais verde e frutos coletados no final da safra com a casca amarela. Os frutos foram coletados com pedúnculo, ao acaso de um pomar comercial com sete anos de idade, localizado no município de Perdões, região Sul do Estado de Minas Gerais. Foram realizadas duas colheitas, uma no mês de Abril quando os frutos estavam com a casca verde, mas já estavam aptos para o consumo; e outra mais no final da safra no mês de Julho quando os frutos já estavam com a casca bem amarela. Os frutos coletados foram levados ao Laboratório de Bioquímica da Universidade Federal de Lavras, onde foram selecionados 100 (cem frutos de tamanho uniforme e com ausência de injúrias a cada colheita, estes foram lavados com uma solução de hipoclorito de sódio a 1%, protocolados, pesados, medidos e realizadas as leituras de cor. Os frutos foram então armazenados em refrigerador a 5 ± 2° C e umidade relativa de 85 ± 3% por um período de 28 dias, e as análises foram realizadas nos frutos e no suco dos frutos aos 0, 7 14, 21 e 28 dias de armazenamento. O delineamento experimental utilizado foi o inteiramente casualizado com 5 repetições de 4 frutos . Os resultados mostraram que quase todas as análises realizadas apresentaram variações significativas. A perda de massa, os diâmetros transversais e longitudinais apresentaram diminuições significativas com os dias de armazenamento refrigerado. Os açúcares também tiveram variações significativas com tendência de aumento com os dias de armazenamento.O pH não apresentou variações significativas. Os teores de Vitamina C não apresentaram varia

  18. Inclusion levels of soluble carbohydrate in the diet for Pacific white shrimp (Litopenaeus vannamei Boone, 1931 - doi: 10.4025/actascianimsci.v33i4.10879 Níveis de inclusão de carboidrato solúvel em dietas para camarão branco do pacífico (Litopenaeus vannamei Boone, 1931 - doi: 10.4025/actascianimsci.v33i4.10879

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    Neyva Ribeiro Ferraz

    2011-09-01

    Full Text Available It was evaluated different inclusion levels of carbohydrates (20, 25, 30 and 35% on the performance of Pacific white shrimp fed isoprotein diets (33% crude protein and isoenergy (4,700 kcal kg -1 diets in a complete randomized design with four treatments (n = 3. The experiment was performed in a pond with 1,200 m 2 and 1.5 m depth, where food intake, daily weight gain, feed conversion, protein efficiency ratio, and retention rates of crude protein and gross energy and survival rate of shrimp were evaluated. For the different inclusion levels of soluble carbohydrates, there were no differences for feed conversion, and retention rates of crude protein and gross energy and survival rate, resulting in means of 1.22:1, 1.2%, 0.74 and 95.0%, respectively. Inclusion levels of soluble carbohydrate significantly influenced the other variables. It was observed that increasing levels of soluble carbohydrate quadraticaly influenced (p Litopenaeus vannamei weighing more than 9 gForam avaliados diferentes níveis de inclusão de carboidratos (20, 25, 30 e 35% sobre o desempenho do camarão branco do pacífico alimentado com rações isoproteicas (33% de proteína bruta e isoenergéticas (4.700 kcal kg-1 em um delineamento inteiramente casualizado com quatro tratamentos (n = 3. O experimento foi executado em um viveiro de 1.200 m 2 e 1,5 m de profundidade, onde foi avaliado o consumo de ração, o ganho de peso diário, a conversão alimentar aparente, a taxa de eficiência proteica, coeficientes de retenção da proteína bruta e energia bruta e a taxa de sobrevivência do camarão. Para os diferentes níveis de inclusão de carboidrato solúvel, não foram observadas diferenças para conversão alimentar aparente, coeficientes de retenção da proteína bruta e energia bruta e taxa de sobrevivência, obtendo-se médias, respectivamente, 1,22:1; 1,21; 0,74 e 95,00%. Houve influência dos níveis de inclusão de carboidrato solúvel sobre as demais vari

  19. Efeito da adição do melaço na relação carbono/nitrogênio no cultivo de camarão Litopenaeus vannamei na fase berçário - DOI: 10.4025/actascibiolsci.v31i4.4496 Effect of molasses addition on carbon/nitrogen ratio in the nursery phase of Litopenaeus vannamei shrimp culture - DOI: 10.4025/actascibiolsci.v31i4.4496

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    Diogo Bessa Neves Spanghero

    2009-08-01

    Full Text Available Investigou-se o efeito da adição do melaço nas relações carbono:nitrogênio (C:N sobre o desempenho zootécnico do camarão Litopenaeus vannamei, na fase berçário, quando cultivado sem renovação de água. As relações do C:N foram avaliadas nas proporções de 25:1 (25M, 15:1 (15M e o controle (0M, sem aplicação de carbono, em delineamento experimental inteiramente casualizado, com quatro repetições. Pós-larvas (PL com peso inicial de 2,5 ± 0,5 mg, foram estocadas em 12 tanques (800 L volume útil, em densidades de 6,25 PL L-1, durante 42 dias de cultivo. Ao final do cultivo, os pesos finais dos camarões dos tratamentos 25M (532,0 mg e 15M (540,0 mg foram superiores (p L. vannamei cultivadas na fase berçário sem renovação de águaThe effect of molasses addiction on carbon:nitrogen ratios (C:N on the performance of Litopenaeus vannamei shrimp during the nursery phase cultured without water exchange was investigated. The C:N ratios were evaluated in 25:1 (25M and 15:1 (15M proportion and a control (0M, with no carbon source addition, in a randomized experimental design with four replicates. Post-larvae (PL with initial weight of 2.5 ± 0.5 mg were stocked in 12 tanks (800 L net volume, at the density of 6.25 PL L-1 during 42 culture days. At the end of culture, the shrimps weights on 25M (532.0 mg and 15M (540.0 mg treatments were higher (p L. vannamei post-larvae cultured without water exchange.

  20. Effects of the recombinant crustacean hyperglycemic hormones rCHH-B1 and rCHH-B2 on the osmo-ionic regulation of the shrimp Litopenaeus vannamei exposed to acute salinity stress.

    Science.gov (United States)

    Camacho-Jiménez, Laura; Díaz, Fernando; Sánchez-Castrejón, Edna; Ponce-Rivas, Elizabeth

    2018-03-26

    The Pacific white shrimp Litopenaeus vannamei is a euryhaline organism that copes with salinity fluctuations in the environment; therefore, its osmotic and ionic regulation abilities are vital. Osmoregulation may be controlled by the crustacean hyperglycemic hormone (CHH), a neuropeptide mainly expressed in the eyestalks. In L. vannamei, CHH-B1 and CHH-B2 are CHH isoforms isolated from the eyestalks whose expression is influenced by environmental salinity. It has been suggested that they are involved in the response to salinity stress. To clarify this, we investigated the effect of the recombinant peptides, rCHH-B1 and rCHH-B2, on the osmo-ionic regulation of shrimp acutely exposed to different salinity conditions (8, 26 and 45‰). Both rCHHs promoted differential effects on the osmoregulatory capacity (OC) and the ionoregulatory capacity (IC) for hemolymph Na + and Cl - during iso-osmotic (26‰) and hyper-osmotic (45‰) transfers. These changes were linked to the changes observed in Na + /K + ATPase and carbonic anhydrase gene expression in gills, especially under high salinity conditions, suggesting that the hormones may regulate the expression of these genes. Glucose and protein levels measured during acute salinity transfer suggest their roles as sources of metabolic energy for osmotic regulation or as organic osmolytes. These results taken together suggest that both the CHH-B1 and CHH-B2 peptides are important regulators of the physiological response of L. vannamei to acute salinity fluctuations.

  1. Bactérias entéricas presentes em amostras de água e camarão marinho Litopenaeus vannamei oriundos de fazendas de cultivo no Estado do Ceará, Brasil

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    Lucélia Sabóia Parente

    2011-02-01

    Full Text Available Foram realizados a quantificação de coliformes totais (CT e termotolerantes (CTT, isolamento e identificação de coliformes, e pesquisa de Salmonella em 28 amostras de água e 28 de camarão da espécie Litopenaeus vannamei, oriundas de duas fazendas de cultivo localizadas no Estado do Ceará, Brasil. Nenhuma amostra de água apresentou índice de CTT acima do limite de 2.500/100 mL preconizado pela legislação para águas salobras destinadas ao cultivo de organismos para fins de consumo. O Número Mais Provável (NMP/g de CTT das amostras de camarão variou de <3 a 2,9 x 10(4. A maior frequência de isolamento de coliformes nas amostras de água e camarão foi a da espécie Escherichia coli. Em apenas três (5,35%, das 56 amostras analisadas, foi detectada a presença de Salmonella sorovar Newport e S. Saintpaul. Apesar do baixo índice de CTT e da baixa incidência de salmonela, a presença dessas bactérias entéricas em ambientes de cultivo de peneídeos é preocupante, uma vez que podem provocar infecções em humanos.

  2. AvaliaÃÃo de subprodutos do abate de animais terrestres e de resÃduos do processamento de peixes como fonte de proteÃna em raÃÃes para o camarÃo branco do pacÃfico, Litopenaeus vannamei (BOONE, 1931)

    OpenAIRE

    Pedro Henrique Gomes dos Santos

    2013-01-01

    Os efeitos da utilizaÃÃo de subprodutos animais em dietas para o camarÃo Litopenaeus vannamei sÃo pouco conhecidos. O presente estudo avaliou o desempenho zootÃcnico e as respostas alimentares de juvenis do camarÃo L. vannamei quando alimentados com dietas contendo subprodutos do abate de animais terrestres e de resÃduos do processamento de peixes como substitutos parciais ou totais da farinha de peixe. O trabalho foi dividido em duas etapas, uma consistindo do cultivo de camarÃes de 2,03 Â 0...

  3. FRONTERAS DIFUSAS Y ACTORES SOCIALES MESTIZOS: DEBATES CONCEPTUALES Y DESARROLLOS ANALÍTICOS EN TORNO A LOS ESPACIOS DE FRONTERA Y SUS VINCULACIONES CON LOS INDIOS-BLANCOS EN LA REGIÓN DEL CHACO DURANTE LA SEGUNDA MITAD DEL SIGLO XIX

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    Julio César Spota

    2010-12-01

    Full Text Available En la frontera establecida entre el Estado argentino y las distintas parcialidades aborígenes de la región del Chaco durante la segunda parte del siglo XIX, se configuraron identidades étnicas mestizas que escapaban a la simple esquematización de blancos e indios, civilizados y salvajes. La praxis histórica de los actores sociales como los indios-blancos (soldados desertores, criminales fugitivos de la ley, perseguidos y refugiados políticos y comerciantes que fueron incorporados dentro de los grupos indígenas proporciona un espacio privilegiado de reflexión antropológica poco explorado hasta el momento. En el presente artículo nos proponemos determinar las causas históricas que motivaron la migración de los criollos y recuperar la perspectiva de los actores sociales que protagonizaron los hechos estudiados.

  4. Caracterización bioquímica de la enzima bifuncional dihidrofolato reductasa-timidilato sintasa de Leishmania (Viannia y su evaluación como blanco molecular

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    Edison Osorio

    2013-09-01

    Full Text Available Introducción. La dihidrofolato reductasa (DHFR se ha utilizado como blanco molecular en tratamientos antibacterianos, anticancerígenos y antipalúdicos. También, actúa como blanco molecular en Leishmania; sin embargo, no existen reportes de la enzima bifuncional en especies de Leishmania (Viannia. Materiales y métodos. Se ha aislado y expresado en Escherichia coli el gen que codifica para la enzima bifuncional DHFR y la timidilato-sintasa (TS de Leishmania braziliensis. La enzima recombinante se purificó y caracterizó, y se evaluó el efecto inhibitorio de algunos antifolatos, así como de cuatro alcaloides aporfínicos. Resultados. El gen se compone de aproximadamente 1.560 pb y codifica un péptido de 58 kDa que contiene los dominios DHFR y TS ligados en una sola cadena polipeptídica. La enzima recombinante DHFR-TS, utilizando el dihidrofolato (H2F como sustrato, presentó valores de Km y Vmax de 55,35± 4,02 (media ± el error estándar de la media y de 0,02 ± 5,34 x 10-4, respectivamente. La enzimar DHFR-TS de L. braziliensis presentó valores de Ki para los antifolatos en el rango de micras. El metotrexato fue el inhibidor más potente de la actividad enzimática (Ki=22,0 mM en comparación del trimetoprim (Ki=33 mM y la pirimetamina (Ki=68 mM. Estos valores de Ki son significativamente más bajos en comparación con los obtenidos para los alcaloides aporfínicos. Conclusión. Los resultados muestran el efecto inhibitorio de los antifolatos sobre la actividad enzimática, lo cual indica que la rDHFR-TS de L. braziliensis podría ser un modelo para estudiar moléculas antiprotozoarias potenciales. doi: http://dx.doi.org/10.7705/biomedica.v33i3.1434

  5. Manuel Grijalvo, Un Obispo Burgales en Filipinas. By Roberto Blanco Andrés. Burgos: Publicaciones de la Excma. Diputación de Burgos, 2011. 88 pages, plates.

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    Grace Liza Y. Concepcion

    2012-12-01

    Full Text Available This brief biography is one among many published works by RobertoBlanco Andrés, a Spanish historian who specializes in the history of religious orders in the Philippines, particularly the Augustinians. The aim of the present work is simple. It is to recount Fray Manuel Grijalvo’s life as a religious priest who occupied key positions in the Augustinian Order and in the Philippine church as bishop of Nueva Caceres (now Naga in the first half of the 19th century, at a time when both the Order and the church in the Philippines were undergoing transition. This succinct narration of an Augustinian bishop’s lifeopens a window through which one can appreciate Philippine history in the 19th century. Although brief, the summary of Grijalvo’s years in the Philippines, spent in Manila and Nueva Caceres, gives a relatively complete view of the challenges that the Catholic Church was facing in this era, both within the Augustinian Order and in the Diocese of Nueva Caceres. This is so because Grijalvo was an Augustinian missionary who also occupied key posts as parish priest in Batangas towns (1814 -1818 and in Pasig (1833 -1839 and 1831-1847. He was also procurator and prior of the Order between 1818 and 1832. In1848, he was appointed bishop of Nueva Caceres, a post he occupied until his death in 1861. His life, therefore, encapsulates the struggles and challenges of the Philippine Catholic Church and the Spanish colonial government.

  6. A novel CHH gene from the Pacific white shrimp Litopenaeus vannamei was characterized and found highly expressed in gut and less in eyestalk and other extra-eyestalk tissues.

    Science.gov (United States)

    Ventura-López, Claudia; Gómez-Anduro, Gracia; Arcos, Fabiola G; Llera-Herrera, Raúl; Racotta, Ilie S; Ibarra, Ana M

    2016-05-15

    The crustacean hyperglycemic hormone (CHH) family is an important group of neuropeptides involved in controlling growth, reproduction, and stress response in decapod species. In this study, a new gene containing 4 exons-3 introns flanked by canonical 5'-GT-AG-3' intron splice-site junctions was isolated from Litopenaeus vannamei. Two full length transcripts of this CHH were isolated from eyestalk and pericardial tissue of males and females using rapid amplification of cDNA ends (RACE). Transcripts sequences were 1578bp in length in males pericardial tissues and in males and females eyestalk with 100% identity, but the transcript isolated from females pericardial tissues was shorter (974bp). The differences in transcripts length is a result of two polyadenylation sites present in the 3'UTR resulting in two transcription termination signals. Transcript sequences encoded one unique protein that can be classified as type I CHH subfamily because of the 4 exons and 3 introns structure, although the CPRP region is not-well conserved and there is no amidation in the C-terminal of the deduced amino acid sequence. Furthermore, there is a glycine inserted in the mature peptide not at position 12 as in type II CHHs but after amino acid 31 and the phylogenetic analysis did not group the peptide within type I, but closer to type II CHHs. We demonstrated by endpoint-PCR, qPCR, and in situ hybridization (ISH), that this gene is expressed in neuroendocrine organs known to express CHHs in penaeid shrimp, including X-organ and optic nerve in eyestalk, supraesophageal ganglion (SoG), but it is also expressed in other organs as gill, gut, pericardial cavity, as well as in terminal ampoule or spermatophore and vas deferens of males. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Cloning and expression of the recombinant crustacean hyperglycemic hormone isoform B2 (rCHH-B2) and its effects on the metabolism and osmoregulation of the Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Camacho-Jiménez, Laura; Sánchez-Castrejón, Edna; Díaz, Fernando; Aguilar, Manuel B; Muñoz-Márquez, Ma Enriqueta; Ponce-Rivas, Elizabeth

    2017-11-01

    Crustacean hyperglycemic hormones (CHHs) are multifunctional neuropeptides ubiquitous in crustaceans. In Litopenaeus vannamei, CHH-B2 is a CHH eyestalk isoform whose expression has been shown to vary with enviromental conditions, suggesting its relevance for ecophysiological performance of shrimp, controlling processes related to metabolism and osmo-ionic regulation. To study the involvement of CHH-B2 in these processes, we cloned and expressed a recombinant version with a free C-terminal glycine (rCHH-B2-Gly) in the methylotrophic yeast Pichia pastoris. The rCHH-B2-Gly peptide secreted to the culture medium was purified by RP-HPLC and used for in vivo glucose, triglyceride, and osmoregulation dose-response analyses with juvenile shrimp. The peptide was also amidated at the C-terminus using an α-amidating enzyme to produce rCHH-B2-amide. The shrimp showed a dose-dependent effect of rCHH-B2-Gly to hemolymph glucose and triglyceride levels, inducing maximal increases by injecting 500 and 1000pmol of hormone, respectively. Additionally, 10pmol of hormone was sufficient to reduce the hypo-osmoregulatory capacity of shrimp at 35‰. These findings suggest that CHH-B2 has regulatory roles in carbohydrate and lipid metabolism, and a potential involvement in osmoregulation of L. vannamei. Injection of 100pmol of rCHH-B2-amide increased glucose and triglyceride levels by 15 and 28%, respectively in comparison with rCHH-B2-Gly, suggesting an important role for the C-terminal amidation. Additionally, an in silico structural analysis done with the CHH-B1 and rCHH-B2-Gly peptides suggests that the C-terminal region may be relevant for the activity of the L. vannamei isoforms and explain the functional divergence from other crustacean CHH/CHH-like peptides. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Efecto del fotoperiodo y la razón camarón:macroalga en la remoción de nitrógeno amoniacal total por Gracilaria vermiculophylla, en cultivo con Litopenaeus vannamei, sin recambio de agua

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    Adolfo Sánchez-Romero

    2013-11-01

    Full Text Available Se evaluó el efecto del fotoperiodo y la relación de biomasa camarón:macroalga en la remoción de nitrógeno amoniacal total (NAT, por la macroalga Gracilaria vermiculophylla, en cultivo con camarón Litopenaeus vannamei, sin recambio de agua. Se evaluaron cuatro fotoperiodos: 12:12, 14:10, 16:08 y 24:00 h de luz:oscuridad y dos relaciones de biomasa camarón:macroalga, 1:4 y 1:8. El diseño experimental fue factorial de 4x2 con cuatro réplicas por tratamiento. Las menores concentraciones de NAT (0,55 ± 0,17 mg L-1, N-NO² (0,145 ± 0,29 mg L-1 y N-NO3 (3,13 ± 0,73 mg L-1 se registraron en la relación 1:8. Las mayores tasas de crecimiento de la macroalga (3,07 ± 1,44% día-1 se obtuvieron con fotoperiodo de 24:00 h y relación de biomasa 1:8. La mayor tasa de incorporación de N en la macroalga (0,31 ± 0,12 mg N L-1 día-1 ocurrió en las mismas condiciones. El crecimiento del camarón fue similar al reportado en sistemas tradicionales de recirculación. La integración de G. vermiculophylla en el sistema sin recambio, fue capaz de mantener los niveles de compuestos nitrogenados tóxicos en niveles favorables para el crecimiento de L. vannamei.

  9. Avaliação de bacterina e Lactobacillus plantarum frente à infecção experimental por Vibrio harveyi em pós-larvas de Litopenaeus vannamei

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    Celso Carlos Buglione

    2008-12-01

    Full Text Available This study aimed to verify the effect of probiotics and inactivated cells of bacterias such as Vibrio alginolyticus, Aeromonas salmonicida and Pasteurella multocida in larvae survival of Litopenaeus vannamei, in stress test and experimental infection with Vibrio harveyi. Conic tanks of 30 L, were stocked with 400 post-larvae stage five. Four experimental treatments with triplicates consisted of: 1: commercial feed (control, 2: commercial feed plus bacterin by oral administration in artemia, 3: commercial feed plus bacterin by immersion administration, 4: commercial feed with Lactobacillus plantarum inoculation. Bacterin application was conducted 6h before the infection and stress test, while probiotic administration was for 15 days before challenges. In stress test, post-larvae of treatment 4 (commercial feed supplemented with Lactobacillus plantarum with reached the highest survival rate (87,86 ± 2,35% followed by the ones of treatment 3 and 2 (bacterim by immersion and bacterim by oral administration in artemia with 81,54±1,50% and 80,16 ± 2,15%, respectively, which were superior to the control treatment (72,63 ± 3,34%. Next to V. harveyi challenge, animals from treatment 3 presented the highest survival rate (79,60 ± 7,12% followed by treatments 4 (69,60 ± 10,43%, 2 (65,60 ± 5,18% and control (56,4 ± 5,58%. All treatments were different from control. The present results demonstrate the possible use of L. plantarum and bacterin as promoters in survival rates of L. vannamei post-larvae in the stress tests and challenges with Vibrio harveyi.

  10. SEBARAN INFEKSI TAURA SYNDROME, INFECTIOUS MYONECROSIS, DAN Penaeus vannamei NERVOUS VIRUS (TSV, IMNV, DAN PvNV PADA BUDIDAYA UDANG Litopenaeus vannamei DI JAWA BARAT, JAWA TIMUR, DAN BALI

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    Isti Koesharyani

    2015-09-01

    Full Text Available Pada budidaya udang introduksi Litopenaeus vannamei, virus merupakan penyakit yang memberi dampak cukup merugikan dan menimbulkan kematian massal budidaya udang vaname. Tujuan penelitian ini adalah untuk mengetahui sebaran adanya infeksi virus di beberapa daerah budidaya udang L. vannamei, di Jawa Timur (Bangil, Banyuwangi, Situbondo, Bali, dan Jawa Barat (Karawang dan Mauk-Tangerang. Jenis virus yang dianalisis adalah Taura Syndrome (TSV, Infectious Myonecrosis (IMNV, dan Penaeus vannamei Nervous Virus (PvNV dan merupakan golongan RNA virus. Sebanyak 5-10 ekor sampel diambil dari setiap daerah secara individu berupa jaringan insang, pleopod, dan daging, disimpan dalam RNAlater. Selanjutnya sampel sampel tersebut dianalisis di Laboratorium Kesehatan Ikan, Pusat Penelitian dan Pengembangan Perikanan Budidaya, Jakarta. Metode analisis menggunakan Reverse-Transcription Polymerase Chain Reaction (RTPCR dengan spesifik primer: TSV (230 bp, IMNV-1 (600 bp, PvNV-1 (339 bp. Hasil analisis RT-PCR, menunjukkan bahwa dari 56 sampel, ternyata infeksi TSV diperoleh di lokasi budidaya udang di Bangil, Banyuwangi, dan Bali. Sementara, kasus infeksi IMNV terdapat di Banyuwangi dan Bali, sedangkan infeksi PvNV yang merupakan penyakit baru tidak diperoleh dari semua sampel yang ada. Beberapa sampel uji  menunjukkan multi infeksi secara alami antara TSV-IMNV yang berasal dari budidaya di Banyuwangi. Mengingat, kasus infeksi PvNV belum pernah ada di Indonesia, maka perlu aturan tata cara impor atau pengawasan tentang udang vaname agar tidak terjadi introduksi penyakit virus baru ke Indonesia.

  11. Profile of the geriatric patient treated in the Unidad de Incontinencia Urinaria del Hospital Nacional de Geriatria y Gerontologia 'Dr. Raul Blanco Cervantes' in the period from January 1, 2012 to June 30, 2013

    International Nuclear Information System (INIS)

    Garita Cordero, Andrea

    2014-01-01

    The elderly adult patient is characterized in the Unidad de Incontinencia Urinaria of the Hospital Nacional de Geriatria 'Dr. Raul Blanco Cervantes', during the period between January 1, 2012 and June 30, 2013. The total revision was 513 clinical records of patients treated for the first time in the Urinary Incontinence Unit in the established period. 228 of the cases were excluded. A final sample of 285 patients aged 76 years on average is studied, the predominance is the female sex and the state is widowhood, schooling is Aged 6 and residing in San Jose. In addition, partial dependent patients BLFA (Basic Life Activities) and IALF (Instrumental Activities of Life) are included, in normal cognitive condition according to schooling by MMSE (Mini Mental State Examination) and with risk of depression. As comorbidity they have presented mainly AHT (Arterial hypertension), DM (Diabetes Mellitus) and depression with associated obesity. The patients studied have consumed at least 2 medications simultaneously and usually have had a previous surgical history. Women who have had gynecological obstetric records of pregnancies and births were found to be unrelated to some type of incontinence. Patients have presented defects of the pelvic floor, such as cystocele and rectocele, in men increased prostate size. The most prevalent urinary incontinence has been the mixed, finding association by gender, in women is given more mixed urinary incontinence, followed by stress urinary incontinence; In men, urgent urinary incontinence and urinary incontinence due to overflow. Patients are sent to treatment with electrostimulation of pelvic floor and completed sessions, have shown an improvement in incontinence and quality of life. A more detailed evaluation of the older adult and to evaluate urinary incontinence could give greater results of the capture of patients with positive repercussion in the patient's quality of life. (author) [es

  12. El pago de servicios ambientales como instrumento de gestión ambiental para el abastecimiento sostenible de agua potable a la ciudad de Río Blanco, Nicaragua

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    Yuri Marín

    2006-02-01

    Full Text Available SE REALIZÓ UNA VALORACIÓN ECONÓMICA DE LOS RECURSOS HÍDRICOS DE LA microcuenca “La Golondrina” y se formuló una propuesta de operatoria de Pago de Servicios Ambientales para su conservación. El área de estudio corresponde al Cerro Musún, una reserva protegida bajo co-manejo, principal fuente abastecedora de agua potable a la ciudad Río Blanco. La metodología implicó: elaboración de balance hídrico para determinar la oferta hídrica, identificación de zonas críticas para la estimación de los costos ambientales; valoración contingente para estimar la disposición a pagar de la población (DAP, y revisión del marco legal e institucional que regula los recursos hídricos y las facultades de los municipios para la gestión ambiental. Los resultados del estudio indican que los problemas de la microcuenca son de calidad y no de cantidad de agua. Los costos ambientales ($26,980 dólares/año, permitirían implementar un plan de manejo para reubicación de bebederos, reforestación, letrinificación y medidas para la reconversión productiva de áreas degradadas circundantes, con sistemas agroforestales y silvopastoriles. Los ingresos esperados por DAP ($28,100 dólares/año, permiten cubrir totalmente los costos de conservación; se concluye entonces, que el Fondo Ambiental municipal resulta ser social y económicamente viable de implementar.

  13. CARACTERIZACIÓN DE LA PRODUCCIÓN DE LECHE DE LA CONEJA CON ENFASIS EN LA SUPERVIVENCIA Y CRECIMIENTO DE LA CAMADA EN RAZAS NUEVA ZELANDA BLANCO Y CALIFORNIA

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    Benjamin Gómez-Ramos

    2010-10-01

    Full Text Available El objetivo fue integrar los conocimientos sobre factores genéticos y ambientales que inciden en la producción de leche (PL de la coneja y su efecto en la supervivencia y crecimiento de la camada. Las investigaciones sugieren que la PL se relaciona con el crecimiento y supervivencia del gazapo durante las primeras tres semanas de vida. En cuanto a los aspectos genéticos, se ha estimado que existen diferencias en PL entre razas puras y sus cruzas, además de que el índice de herencia (h2 para PL en Nueva Zelanda Blanco, California y sus cruzas reciprocas es de 0.12%, lo cual establece un mayor efecto ambiental sobre PL. Al parecer la PL alcanza su máxima producción (219.6 g con 12 gazapos/camada y su pico de producción se alcanza al tercer parto y disminuye a partir del quinto parto. Sin embargo, la PL es determinada por: la condición fisiológica de la coneja -lactante-gestante (130 g/día o lactante-no gestante (141 g/día-; el número de pezones, conejas con 10 pezones produjeron 10% más leche que aquellas con 8; la temperatura ambiente, por cada grado centígrado por arriba de 20°C la PL disminuye 7.7 g/día. Los principales factores que influyen sobre la PL de la coneja no son atribuibles a esta. Por lo tanto, es importante controlar la naturaleza aleatoria de la mayoría de los factores no-genéticos que afectan a PL.

  14. Rho GTPasas como blancos terapéuticos relevantes en cáncer y otras enfermedades humanas Rho GTPases as therapeutic targets in cancer and other human diseases

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    Pablo Lorenzano Menna

    2010-12-01

    Full Text Available Las Rho GTPasas son una familia de proteínas clave en la transmisión de señales provenientes del exterior celular hacia efectores intracelulares tanto citoplasmáticos como nucleares. En los últimos año ha habido un desarrollo vertiginoso de múltiples herramientas genéticas y farmacológicas, lo que ha permitido establecer de manera mucho más precisa las funciones específicas de estas proteínas. El objetivo de la presente revisión es hacer foco en las múltiples funciones celulares reguladas por las Rho GTPasas, describiendo en detalle el mecanismo molecular involucrado. Se discute además la participación de estas proteínas en diversas enfermedades humanas haciendo énfasis en su vinculación con el cáncer. Por último, se hace una actualización detallada sobre las estrategias terapéuticas en experimentación que tienen a las Rho GTPasas como blancos moleculares.Rho GTPases are a key protein family controlling the transduction of external signals to cytoplasmatic and nuclear effectors. In the last few years, the development of genetic and pharmacological tools has allowed a more precise definition of the specific roles of Rho GTPases. The aim of this review is to describe the cellular functions regulated by these proteins with focus on the molecular mechanism involved. We also address the role of Rho GTPases in the development of different human diseases such as cancer. Finally, we describe different experimental therapeutic strategies with Rho GTPases as molecular targets.

  15. Ante el traspaso de dos decanos muy relevantes de la Facultad de Derecho de Málaga (Antonio Pérez de la Cruz Blanco y Alejandro Rodríguez Carrión

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    Manuel J. Peláez

    2013-03-01

    Full Text Available Tratamos de hacernos eco en las presentes líneas de dos Decanos de la Facultad de Derecho de la Universidad de Málaga, fallecidos en circunstancias diversas en el año 2009, que marcaron de forma extraordinaria la evolución de dicha Facultad. El primero, Antonio Pérez de la Cruz Blanco, catedrático de Derecho mercantil, y el segundo Alejandro Rodríguez Carrión, catedrático de Derecho internacional público y Relaciones internacionales, que estuvo al frente del decanato durante cuatro periodos, de desigual extensión y espaciados en el tiempo los dos primeros de los segundos. El actual decano de la Facultad de Derecho, Juan José Hinojosa, con buen criterio, ha deseado homenajear a ambos, pero lamentablemente solo al segundo se le han organizado actos de momento en la Facultad de Derecho de Málaga. A Pérez de la Cruz se le ha tributado un homenaje en la Facultad de Derecho de la Universidad de Alcalá de Henares y se ha publicado una miscelánea de trabajos en su homenaje. A Rodríguez Carrión llevamos tres años y diez meses insistiendo que se le organice una miscelánea en dos volúmenes recogiendo trabajos científicos de su área de conocimiento en el primero y de Varia Iuridica en el segundo. De momento nada se ha avanzado al respecto de lo que me alcance la noticia.

  16. Performance and immunological resistance of Litopenaeus vannamei fed a β-1,3/1,6-glucan-supplemented diet after per os challenge with the Infectious myonecrosis virus (IMNV

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    Hassan Sabry Neto

    2015-05-01

    Full Text Available This study determined the efficacy of a β-1,3/1,6-glucan source on the survival, growth, and immunological responses of Litopenaeus vannamei challenged with infectious myonecrosis virus (IMNV. The work was conducted in 20 tanks of 500 L with clear water with constant recirculation and aeration. Shrimp weighing 2.6±0.4 g were stocked at 100 animals/m2 and reared for 10 weeks. Viral challenge occurred through oral administration of IMNV positive extract (1.82 £ × 103 copies of IMNV/µL RNA during three consecutive days, twice a day. One treatment and three controls, with five replicatetanks each, were used, as follows: Com - commercial diet fed to non-challenged shrimp (NCS; Ref - lab-manufactured basal diet, deprived of β-1,3/1,6- glucan fed to NCS; IMNV-Ref - basal diet fed to IMNV-challenged shrimp; and, IMNV-BetG - basal diet with 1,000 mg/kg of a β-1,3/1,6-glucan. Per os challenge began on the 29th day of rearing (4.93-g to 6.92-g shrimp. Shrimp were effectively contaminated with IMNV. Animals were highly susceptible to IMNV four weeks after the first day of infection when mortality peaked. Shrimp in the Com treatment (69.5±12.7% reached the highest survival, but it did not differ from the Ref group (57.9±9.21%. Shrimp under the IMNV-Ref treatment achieved the lowest survival (23.2±5.76%. Survival for IMNV-BetG (48.1±8.53% was significantly higher than IMNV-Ref, and similar to the Ref group. Total hemocyte count was significantly affected by treatment and hemolymph sampling time, while serum protein concentration and phenoloxidase activity did not change. Addition of 1,000 mg/kg of β-1,3/1,6-glucan in a diet for L. vannamei enhances shrimp survival when orally exposed to IMNV. No signs of immunological fatigue or detriment in growth are detected when the species is continually exposed to this diet.

  17. The Black-White Achievement Gap Revisited Revisitando la brecha en el rendimiento de estudiantes negros y blancos Revisitando a defasagem de rendimento academico dos estudantes negros e brancos

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    Henry Braun

    2010-09-01

    Full Text Available This study examines trends in Black student achievement and in the Black-White achievement gap over the period 2000 to 2007, employing data from ten states drawn from the NAEP Grade 8 mathematics assessments. Results are obtained for three levels of aggregation: the state, school poverty stratum within the state, and schools within poverty stratum. In addition, information on the ten states’ education policies for the period 1998 to 2005 was compiled. States relative ranks on the overall strength of their reform efforts were compared to their relative ranks with respect to their success in improving Black student achievement and in reducing the Black- White achievement gap. This study constitutes an extension of earlier work that considered the same issues for the period 1992 to 2000 and, thus, offers a unique comparison between the pre-NCLB era and the present one. Although the ten states certainly differed in their outcomes, the general picture at all three levels of aggregation is quite clear: The achievement gaps are substantial and the introduction of federally mandated high stakes test-based accountability through No Child Left Behind has had a very modest impact on the rates of improvement for Black students and on the pace of reductions in the achievement gaps between Black students and White students. Moreover, there was only a weak association between states’ policy rankings and their rankings related to test results. It appears there is a need for both fresh thinking on education reform and a more concerted effort to collect comprehensive longitudinal information on states' education policies.
    Este estudio examina las tendencias en el rendimiento académico de estudiantes negros y la brecha en el rendimiento de los estudiantes negros y blancos en el período 2000 a 2007, utilizando datos de las evaluaciones NAEP de Matemáticas para el octavo grado en diez estados. Se obtuvieron resultados para tres niveles de agregacio

  18. Evaluation of hybrids of So/anum po/yadenium Greenm for white worm resistance in potatoe (Premmotripes voraxHustoche. Evaluación de híbridos de Solanum polyadenium Greenm. Para resistencia al gusano blanco de la papa (Premnotrypes vorax Hustache

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    Trillos G. Ofelia

    1993-12-01

    Full Text Available Four Solanum polyadenlum crossed with Solanum berthanltil, Solanum chacoense and Solanum phureja were evaluated for resistance to the Andean potato weevil (Premnotrypes vorax Hustache. From the set of 397 hybrids avaluated six (1,52%
    were selected for resistance. For other agronomical and biochemical characteristics the most promising hybrids were the 12 th, the 14 th and the 15 th from the second family (498096.1-Solanum polyadenlium x Solanum phureja. The type of resistance found was antibiosis which affects both the development and the reproduction of the insect.Enel presente trabajo se evaluó el grado de resistencia
    al gusano blanco de la Papa (Premnotrypes voraxHustache de los hibrldos obtenidos mediante el cruzamiento de la especie Solanum polyadenium con las especies Solanum berthanltil, Solanum chacoense y Solanum phureja. De un total de 397 hibridos evaluados, se seleccionaron seis (1.5% por su alto nivel de resistencia al gusano blanco. Porotras caracteristicas agronómicas y bioquimicas, se consideraron como losmás importantesen un programa de mejoramiento a los hibridos 12; 14 y 15 de la Familia 2 (498096.1 - hibrido de Solanum polyadenlum x Amarilla Perú - Solanum phureja. El mecanismo de resistencia observado es del tipo antibiosis, el cual afecta la supervivencia, desarrollo y reproducción del insecto.

  19. Los medios como blancos de guerra

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    Carlos Gutiérrez Aparicio

    2015-01-01

    Full Text Available Trata sobe el golpe militar de Fujimori (1992 cuando los medios de comunicación fueron intervenidos, se clausuraron dos radios y se censuró la información periodística, aparecieron y desaparecieron empresas periodísticas, se saturan los diales de emisoras radiales y agudizó la competencia por una frecuencia de teledifusión. En un país desarticulado por la crisis, es muy difícil reconocer en qué medida los medios masivos son actores estelares del proceso.

  20. Evaluación de la toxicidad de proteínas de Bacillus thuringiensis Berliner hacia el gusano blanco de la papa Premnotrypes Vorax Hustache Bacillus thuringiensis Berliner proteins toxicity evaluation against andean Po tato Weevil Premnotrypes Vorax Hustache

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    Martínez O. Wilson

    2002-08-01

    Full Text Available El presente estudio estableció una metodología de bioensayo para determinar la actividad tóxica que las proteínas patrón Cry3Aa, Cry3Ba, Cry3Bb, Cry3Ca y Cry7Aa de B. thuringiensis pudieran tener sobre larvas del gusano blanco de la papa Premnotrypes vorax Hustache, plaga de gran importancia en las diferentes zonas productoras de papa en Colombia. Los bioensayos fueron realizados empleando papa como sustrato alimenticio en forma de tubérculos o como puré y las proteínas mencionadas se emplearon en forma de
    suspensión espora-cristal, solubilizadas y/o procesadas
    enzimáticamente. La metodología de bioensayo más adecuada consistió en cubos de tubérculos de papa impregnados superficialmente con las proteínas de B. thuringiensis. Aunque se observó una ligera mayor actividad de las proteínas Cry3Aa y Cry3Ca esta no superó el 10%. No se obtuvo actividad tóxica de ninguna de las proteínas evaluadas cuando fueron procesadas con la enzima tripsina o solubilizadas a un pH de 4.1. Los resultados indicaron que las proteínas Cry de Bt empleadas no presentan actividad tóxica significativa sobre larvas de P Vorax. Se plantea la hipótesis que esto es debido a que las proteínas provienen de cepas
    aisladas en otras regiones del mundo; por 10 tanto, nuevos
    estudios al respecto deberán evaluar proteínas producidas
    por cepas nativas aisladas de áreas de cultivo donde P vorax se presente como plaga de forma natural.
    The present work stablished a bioassay methodology in order to determine B. thuringiensis Cry3Aa, Cry3Ba, Cry3Bb, Cry3Ca and Cry7 Aa standard proteins toxicity against Andean potato weevil (Premnotrypes vorax Hustache. This insect is a very important pest in almost all potato fields in Colombia. Bioassays were done using natural diet as potato tuber pieces or potatoe flour. Proteins were used as crystals, solubilized or enzimatically processed. Best bioassay used potato pieces superficially

  1. Gramáticas de la memoria. Variaciones en torno a la transición española en cuatro novelas recientes (1985-2000: Luna de lobos, Beatus ille, Corazón tan blanco y La caída de Madrid

    Directory of Open Access Journals (Sweden)

    José Manuel López de Abiada

    2014-06-01

    Full Text Available Nos proponemos abordar aspectos relacionados con la memoria histórica en novelas recientes de cuatro autores españoles significativos para considerarlas y analizarlas desde criterios consustanciales a la transición española. Las obras elegidas son Luna de lobos (de Julio Llamazares, Beatus ille (de Antonio Muñoz Molina, Corazón tan blanco (de Javier Marías y La caída de Madrid (de Rafael Chirbes. No se nos escapa la ausencia de novelas de autores de la envergadura y el significado de Juan Marsé, Manuel Vázquez Montalbán, Juan Iturralde, José Luis Cebrián o Juan Eduardo Zúñiga.

  2. FRONTERAS DIFUSAS Y ACTORES SOCIALES MESTIZOS: DEBATES CONCEPTUALES Y DESARROLLOS ANALÍTICOS EN TORNO A LOS ESPACIOS DE FRONTERA Y SUS VINCULACIONES CON LOS INDIOS-BLANCOS EN LA REGIÓN DEL CHACO DURANTE LA SEGUNDA MITAD DEL SIGLO XIX

    Directory of Open Access Journals (Sweden)

    Julio César Spota

    2010-12-01

    Full Text Available ABSTRACT: In the frontier drawn between the Argentine State and the different aborigine groups of the Chaco region during the second half of the XIX century, different half-breed social identities that escape simple dichotomies between whites and Indians, civilized people and savage people were formed. The historical practice of considering social actors as white Indians (deserting soldiers, fugitive criminals, political exiles and refugees, as well asmerchants who were incorporated to the Indian groups provides a privileged space for anthropological observation which has been relatively little explored until now. This paper investigates the historical reasons that motivated the migration of Creoles and recovers the perspective of social actors who took a leading role in the facts studied.RESUMEN: En la frontera establecida entre el Estado argentino y las distintas parcialidades aborígenes de la región del Chaco durante la segunda parte del siglo XIX, se configuraron identidades étnicas mestizas que escapaban a la simple esquematización de blancos e indios, civilizados y salvajes. La praxis histórica de los actores sociales como los indios-blancos (soldados desertores, criminales fugitivos de la ley, perseguidos y refugiados políticos ycomerciantes que fueron incorporados dentro de los grupos indígenas proporciona un espacio privilegiado de reflexión antropológica poco explorado hasta el momento. En el presente artículo nos proponemos determinar las causas históricas que motivaron la migración de loscriollos y recuperar la perspectiva de los actores sociales que protagonizaron los hechos estudiados.

  3. CURRENT STATE OF CONSERVATION, FIRST PHOTOGRAPHIC RECORD AND POPULATION ESTIMATION OF THE COASTAL JAGUAR (Panthera onca centralis AND RECORDS OF COMPANION FAUNA OF MEDIUM-SIZED AND HIGHER MAMMALS IN THE PROTECTED FOREST CERRO BLANCO OF THE CHONGÓN COLONCHE MOUNTAIN RANGE, GUAYAQUIL – ECUADOR

    Directory of Open Access Journals (Sweden)

    Miguel Saavedra

    2013-12-01

    Full Text Available The Chongón-Colonche Mountain Range is important for their goods and environmental services, its high biodiversity, and being one of the few coastal regions of Ecuador, which still houses the coastal Jaguar Panthera onca centralis. In the Forest Protector Cerro Blanco (BPCB, last Southeast extension of the mountain chain, it was developed the field research through the data collection with direct and indirect medium-sized and higher mammals’ records. Besides a Cuddeback Digital camera trap was used, by selecting a sampling point within a probable route of the jaguar. Inspections in a nearby quarry were made to observe traces of major feline registries. The same consolidated past sightings or evidence of witnesses which complemented the study for the determination of the status of the species in the BPCB. The study showed indirect and direct records of white-tailed deer, peccaries, raccoons, agoutis, wild rabbits, howler monkeys, Capuchin white or monkeys, agouti, bears Anteaters and Jaguars from the coast for which it is considered that the BPCB is probably a meeting place between two individuals; however, it is important to note that the results presented are preliminary.

  4. Registro de pecarí de labios blancos (Tayassu pecari en la región de la laguna de Términos, Campeche, México White lipped pecaries (Tayassu pecari recorded in the area of Términos Lagoon Campeche, México

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    Mircea G. Hidalgo-Mihart

    2012-09-01

    Full Text Available Se registró fotográficamente al menos un grupo de pecaries de labios blancos (Tayassu pecari en la Selva La Montaña, al suroeste del área de Protección de Flora y Fauna Silvestre Laguna de Términos, Campeche, México. El registro se realizó utilizando trampas cámara. La presencia de esta especie muestra la importancia que tiene la región Selva La Montaña para la conservación de la biodiversidad, especialmente para las especies protegidas.We report at least one group of white lipped peccaries (Tayassu pecari in Selva La Montaña located in the southwestern portion of the Términos Lagoon Flora and Fauna Protection Area, Campeche, Mexico. The record was obtained using camera traps. The presence of this species in the area shows the importance of the region for biodiversity conservation, particularly for protected species.

  5. Desenvolvimento vegetativo e produção de óleo essencial de patchouli (Pogostemon cablin (Blanco Benth. após a aplicação de ácido giberélico e extrato de alga marinha

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    R.C. Storck

    2013-01-01

    Full Text Available Pogostemon cablin (Blanco Benth., comumente conhecido como patchouli, é uma espécie aromática utilizada pela indústria de perfumes devido a fragrância amadeirada e propriedade fixadora do óleo essencial. Fatores que afetam o teor dos constituintes do óleo essencial devem ser avaliados visando obter matéria prima de melhor qualidade. O experimento foi conduzido em casa de vegetação com delineamento inteiramente casualizado em esquema fatorial 2x3 e 4 repetições, sendo dois níveis de giberelina (0 e 200 mg L-1 e três níveis de extrato de alga marinha (0, 15 e 30 mg L-1. A aplicação dos reguladores vegetais foi realizada 30 dias após o plantio das mudas, e a avaliação do experimento deu-se 45 dias após a aplicação dos tratamentos. Não houve diferença significativa para a altura das plantas, a concentração de extrato de alga marinha de 15 mg L-1 promoveu aumento no número de folhas em relação à testemunha e a concentração de 30 mg L-1 promoveu decréscimo. Em relação à área foliar e ao número de folhas houve relação inversa para os níveis de extrato de alga marinha, onde o tratamento com maior concentração do regulador resultou em aumento da área foliar e redução do número de folhas. O teor de óleo essencial foi superior após a aplicação de 15 mg L-1 de extrato alga marinha isoladamente ou combinado com GA3. A produtividade do óleo essencial também aumentou com a aplicação 15 mg L-1 de extrato de alga marinha e quando utilizado somente GA3. A utilização de extrato de alga marinha aumentou a concentração de patchoulol no óleo essencial.

  6. Teatro: Un gato blanco, melenudo de ojos azules

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    José Pubén

    1969-01-01

    Full Text Available La escena se presenta simultáneamente dividida en dos partes: Trata de recrear, ante el público, los dos cuartos de un apartamento. La acción transcurre, a lo largo de toda la obra, en uno u otro cuarto o en ambos simultáneamente. Es el mismo decorado para las tres escenas. Un apartamento con muebles y lámparas con el estilo de la época del art noveau.

  7. Crónica roja: ni blanco ni negro.

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    Orlando Pérez

    2015-01-01

    Full Text Available Frente a la interrogante de ¿Qué hacer ante la sentencia que los reos reciben de los medios de comunicación antes de que el juez instaure el proceso? y ¿Cómo enfrentar responsablemente las diversas presiones que tiene el cronista de sucesos? el autor plantea algunas posibilidades: más que los hechos, los procesos; antes de juzgar primero investigar; reportar el fenómeno no el delito, entre otras. Relieva el valor de la Ética en todo trabajo de un reportero de la sección judicial.

  8. Recovery of naringin from kinnow (Citrus reticulata Blanco) peels by ...

    Indian Academy of Sciences (India)

    citrus) peels,which is a waste. The kinnow peels were boiled with water to extract naringin into water. It was adsorbed on an indigenous macroporous resin, Indion PA-500. Naringin was recovered from the saturated resin by desorption with ...

  9. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco Benth.

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    Bhanuz Dechayont

    2017-01-01

    Full Text Available Pogostemon cablin (Lamiaceae has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙- and nitric oxide (NO production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC, and minimum bacterial concentration (MBC. The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

  10. Cytotoxicity and Apoptotic Activity of Ficus pseudopalma Blanco ...

    African Journals Online (AJOL)

    Keywords: Ficus pseudopalma, Cytotoxicity, Apopotic, human prostate PRST2 cancer cell, Lupeol,. Quercetin. Tropical Journal of Pharmaceutical ... and antioxidant properties of the leaves of the plant were demonstrated by promoting ..... colorectal HT29 cancer cell lines have been reported [6,14-17]. However, the study on ...

  11. El Centro Pompidou: elefante sí pero no blanco

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    Héctor Sánchez

    1982-10-01

    Full Text Available Hace algún tiempo tropecé con un excelente tallador de maderas, centroamericano por los cuatro lados y amigo en las jornadas que por aquí se viven. De primera mano me contó que habia pasado un par de meses en París, vengando como se acostumbra, las restricciones de días anteriores. Intentando sumarme a su complacencia le recordé todos esos museos que debía haber visitado, por estricto deber profesional. Con la sangre metida en el rostro y algo tartamudeante me confesó que en eso habia fallado.

  12. Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae

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    Librado A Santiago

    2014-02-01

    Conclusions: The identified lupeol in F. pseudopalma may be responsible for the exhibited antioxidant property of the plant. Furthermore, knowing the antioxidant capability of the plant, F. pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases.

  13. Cytotoxicity and Apoptotic Activity of Ficus pseudopalma Blanco ...

    African Journals Online (AJOL)

    The presence of terpenoid lupeol and flavonoid quercetin was determined through high performance liquid chromatography (HPLC). The cytotoxic and apoptotic effects of different concentrations of FP extracts on PRST2 cells and on non-cancerous human foreskin surface epithelial (hFSE) cells were determined by 3-[4, ...

  14. The Ghost of Blanco: A Note to Ugo Mattei

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    Michele Surdi

    2012-11-01

    Full Text Available By noting the end of the dualism between public and private law, between state and international law and between goods and services, the article comments and interprets Ugo Mattei’s doctrine about new forms of property. Cognitive transformations, both technological and social, do not prelude to a new and different juridical intermediation as guarantee of the communal regime of goods. These new forms of property, when confronted with the domain that private economic property continues to exercise, do not announce neither the dissolution of the monopoly of state coercion, nor the advent of a merely administrative management.

  15. Utilización de harina de frutos y hojas del árbol del pan (Artocarpus altilis en la ceba de conejos Nueva Zelanda Blanco Utilization of fruit and leaf meals from breadfruit tree (Artocarpus altilis for fattening New Zealand White rabbits

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    Coralia S Leyva

    2012-12-01

    Full Text Available Se utilizaron 36 conejos de la raza Nueva Zelanda Blanco, de 35 días de edad y 506 g de peso vivo, con el objetivo de evaluar el efecto de tres dietas en los indicadores productivos y económicos de los animales durante la ceba. Las dietas consistieron en: 1 pienso comercial más forraje fresco de glycine (Neonotonia wightii, como control; 2 harina de frutos del árbol del pan (Artocarpus altilis más glycine; y 3 pienso integral compuesto por una mezcla de harina de frutos y hojas del árbol del pan, más vitaminas y minerales. Se realizó un análisis de varianza, según un diseño completamente aleatorizado, con tres tratamientos y cuatro repeticiones. Después de 90 días en ceba, el peso vivo al sacrificio fue de: 2 347, 2 223 y 2 127 g/conejo, respectivamente; la ganancia media, de 20, 19 y 18 g/día; y la viabilidad fue del 100% en todos los tratamientos. Económicamente, el pienso balanceado con harina de frutos y hojas del árbol del pan tuvo una utilidad de 1,05 USD/conejo cebado, el sistema de alimentación con harina de frutos del árbol del pan más follaje de glycine alcanzó utilidades de 0,89 USD/conejo cebado; mientras que el sistema control generó pérdidas económicas, debido a los altos costos de las materias primas que componían el pienso convencional. Se concluye que los sistemas de alimentación alternativos que emplean la harina de frutos y hojas del árbol del pan resultan económica y biológicamente apropiados para los conejos en cebaThirty-six New Zealand White rabbits, 35 days old and with 506 g of live weight were used, in order to evaluate the effects of three diets on the productive and economic indicators of the animals during fattening. The diets consisted in: 1 commercial concentrate feed plus fresh forage of perennial soybean (Neonotonia wightií, as control; 2 breadfruit (Artocarpus altilis meal plus perennial soybean and 3 integral concentrate feed composed by a mixture of fruit and leaf meal from the

  16. ICP11 as Biomarker for WSSV Disease in Litopenaeus vannamei

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    Yuni Kilawati

    2016-03-01

    Full Text Available Environmental pollution could directly reduce water quality for shrimp cultivation. This study aims to determine how the environmental quality, shrimp population and genetic characteristics of shrimp that live in polluted waters in shrimp ponds in East Java associated with WSSV disease that often attacks on cultivated shrimp. The method used was a descriptive exploratory. Data collection was collected by observation and interview with farmers on disease history. Water was sampled for water quality study and characterize its suitability for shrimp cultivations. Shrimps were also sampled for morphological and genetical study for its susceptibility of WSSV. All samples were taken every week during shrimp cultivation in Malang ans Gresik. Morphological study using scoring method to determine the disease stages on shrimp, while for genetical study using specific primer for ICP11 for WSSV detection, since ICP 11 is expressed when WSSV infection is occur. Samples were taken from 2 shrimp ponds in South Malang and Gresik. The overall water quality is good, except for NO2 and TOM in both seawater shrimp ponds which higher than the freshwater/estuary shrimp ponds. Light infections of WSSV were detected in all seawater ponds both in morphology and genetics. However, in the freshwater/estuary pond only shrimps from freshwater/estuary Gresik which showing light WSSV infection genetically, but not in the morphological signs. Early disease detection is important to control the disease spread.

  17. Sperm ultrastructure of shrimps from the family Penaeidae (Crustacea: Dendrobranchiata) in a phylogenetic context.

    Science.gov (United States)

    Camargo, Tavani Rocha; Rossi, Natalia; Castilho, Antonio L; Costa, Rogério C; Mantelatto, Fernando L; Zara, Fernando José

    2017-07-01

    We describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (Penaeini, Parapenaeini and Trachypenaeini). Fragments of the vas deferens of the Penaeidae Farfantepenaeus brasiliensis, Farfantepenaeus paulensis, Litopenaeus schmitti, Parapenaeus americanus, Rimapenaeus constrictus and Xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. The morphological results were contextualized in an evolutionary perspective using molecular markers for the phylogenetic reconstruction of this group. A phylogram was proposed by Bayesian inference based on 1007 bp of 33 sequences of the combined genes (16S rDNA and COI mtDNA) from 27 dendrobranchiate specimens. Our findings show that morphological differences in the sperm ultrastructures of members among the tribes of Penaeidae can be used as a baseline to understand their evolutionary relationships. Individuals from the Penaeini tribe show plesiomorphic characteristics in the sperm ultrastructure compared to the Trachypenaeini tribe from which they were derived, such as shrimp from family Sicyoniidae. The morphological complexity of the sperm of the different penaeid members corroborated with the genetic phylogeny, which showed different clades for each tribe and the close relationship with Sicyoniidae. The sperm features of the selected species studied here reflected their evolutionary history. These features confirm the previous phylogenetic hypothesis and question the monophyly of Penaeidae, which should be verified in the future with a more complete set of representative members of each tribe. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Cuestión de alteridades: la aciaga vida del Tupã guaraní en la literatura de los "blancos" y en la vital versión oral de los mbyá Uma questão de alteridades: a infortunada vida do Tupã guarani na literatura dos brancos e na vital versão oral dos mbyá The Question of Alterity: the Bitter Life of the Guaraní Tupã According to the White Man's Literature and to the Mbyá's Lively Oral Version

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    Irma Ruiz

    2009-12-01

    Full Text Available Durante siglos, parte del mundo letrado estuvo atento a las opiniones de los blancos sobre el pensamiento y las acciones de los guaraní ; eran tiempos exclusivos de la antropología sobre el Otro. En la actualidad, se pretende dar cuenta también de la antropología del Otro sobre nosotros. A fin de mostrar ambas caras, en este artículo se confrontan voces autorizadas de blancos que escribieron sobre los guaraní , con otras de los mbyá-guaraní que hablaron sobre su cultura y la de los blancos. El eje escogido es la figura de Tupã , de cuyo nombre se apropiaron los evangelizadores coloniales para construir su discurso monoteísta. En este juego de alteridades, se analiza primero la infausta "vida literaria" del personaje para luego oír voces de los aún damnificados por ese hecho histórico. Esta confrontación, además de revelar inconsistencias prejuiciosas en algunos escritos y estrategias guaraní para proteger su politeísmo, permite apreciar la magnitud de la violencia simbólica ínsita en la evangelización.Durante séculos, parte do mundo letrado esteve atento às opiniões dos brancos sobre o pensamento e as ações dos guarani; eram tempos exclusivos da antropologia sobre o Outro. No presente, pretende-se dar conta também da antropologia do Outro sobre nós mesmos. Com o objetivo de mostrar essas duas faces, confrontam-se neste artigo vozes autorizadas de brancos que escreveram sobre os guarani com vozes outras dos mbyá-guarani que falaram sobre a sua cultura e a dos brancos. O eixo escolhido é a figura do Tupã, nome que foi apropriado pelos evangelizadores coloniais para construírem seu discurso monoteísta. Em este jogo de alteridades, será analisada, primeiro, a desventurada "vida literária" da personagem, para depois ouvir vozes dos ainda danificados por aquele fato histórico. Este confronto, além de revelar inconsistências preconceituosas em alguns escritos e estratégias guarani protegendo o seu polite

  19. The invasion of five alien species in the Delta do Parnaíba Environmental Protection Area, Northeastern Brazil

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    Daniel Loebmann

    2010-09-01

    Full Text Available Marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. In the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species were detected during dives, shore searches and from the fisheries on the coast of the Delta do Parnaíba Environmental Protection Area, in the States of Piauí and Maranhão, Northeastern Brazil. The species were the bicolor purse-oyster Isognomon bicolor, the whiteleg shrimp Litopenaeus vannamei, the giant river prawn Macrobrachium rosenbergii, the Indo-Pacific swimming crab Charybdis hellerii and, the muzzled blenny Omobranchus punctatus. Ballast water (I. bicolor, C. hellerii, and O. punctatus and aquaculture activities (L. vannamei and M. rosenbergii in adjacent areas are the most likely vectors of introduction. All exotic species found have potential impact risks to the environment because they are able to compete against native species for resources (food and habitat. Isognomon bicolor share the same habitat and food items with the native bivalve species of mussels and barnacles. Litopenaeus vannamei share the same habitat and food items with the native penaeids such as the pinkspot shrimp Farfantepenaeus brasiliensis, the Southern brown shrimp Farfantepenaeus subtilis, and the Southern white shrimp Litopenaeus schmitti, and in the past few years L. vannamei was responsible for a viral epidemics in the cultivation tanks that could be transmitted to native penaeid shrimps. Charybdis hellerii is also able to cause impacts on the local fisheries as the species can decrease the populations of native portunid crabs which are commercialized in the studied region. Macrobrachium rosenbergii may be sharing natural resources with the Amazon River prawn Macrobrachium amazonicum. Omobranchus punctatus shares habit with the native

  20. The invasion of five alien species in the Delta do Parnaíba Environmental Protection Area, Northeastern Brazil.

    Science.gov (United States)

    Loebmann, Daniel; Mai, Ana Cecília G; Lee, James T

    2010-09-01

    Marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. In the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species) were detected during dives, shore searches and from the fisheries on the coast of the Delta do Parnaíba Environmental Protection Area, in the States of Piauí and Maranhão, Northeastern Brazil. The species were the bicolor purse-oyster Isognomon bicolor, the whiteleg shrimp Litopenaeus vannamei, the giant river prawn Macrobrachium rosenbergii, the Indo-Pacific swimming crab Charybdis hellerii and, the muzzled blenny Omobranchus punctatus. Ballast water (I. bicolor, C. hellerii, and O. punctatus) and aquaculture activities (L. vannamei and M. rosenbergii) in adjacent areas are the most likely vectors of introduction. All exotic species found have potential impact risks to the environment because they are able to compete against native species for resources (food and habitat). Isognomon bicolor share the same habitat and food items with the native bivalve species of mussels and barnacles. Litopenaeus vannamei share the same habitat and food items with the native penaeids such as the pinkspot shrimp Farfantepenaeus brasiliensis, the Southern brown shrimp Farfantepenaeus subtilis, and the Southern white shrimp Litopenaeus schmitti, and in the past few years L. vannamei was responsible for a viral epidemics in the cultivation tanks that could be transmitted to native penaeid shrimps. Charybdis hellerii is also able to cause impacts on the local fisheries as the species can decrease the populations of native portunid crabs which are commercialized in the studied region. Macrobrachium rosenbergii may be sharing natural resources with the Amazon River prawn Macrobrachium amazonicum. Omobranchus punctatus shares habit with the native redlip blenny

  1. Monitoring of carcinofauna abundance and diversity during eight years of expressway construction in Santa Catarina, Brazil Ocho años de monitoreo de la abundancia y diversidad de la fauna carcinológica durante la construcción de una carretera en Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Felipe Freitas Junior

    2010-01-01

    Full Text Available Crustaceans are important in estuarine ecosystems, transferring energy to higher trophic levels and contributing to artisanal and industrial fisheries. This paper aims to evaluate the carcinofauna diversity and abundance in Saco dos Limões and how this changed when affected by dredging during the construction of an expressway through South Bay, Florianópolis, Santa Catarina, Brazil. Collections were made onboard a commercial fishing boat for three months, day and night, at six sampling sites from 1997 to 2006. The families Penaeidae and Portunidae were the most diverse in terms of taxa, with the largest species abundances. The dominant species were the shrimps Farfantepenaeus brasiliensis, F. paulensis, and Litopenaeus schmitti, and the crab Callinectes danae. The largest abundances were found in two areas, in the summer and at night. The diversity and equitability Índices showed similar patterns, with the highest values in autumn and winter. Acording to the Jaccard Index, the similarity of the crustacean composition was highest for the years 2005 and 2006 and lowest between 1997 and 2005. The structure of the carcinofauna changed over the years, with high mortalities during dredging operations followed by a recovery in the subsequent years. Our observations indicated that dredging did not have catastrophiçõeffects ñor did it produce long-term disturbances in the carcinofauna of the estuary. It is important to monitor natural resources in order to record the extent and limits of human impacts on the environment.Los crustáceos son importantes en el ecosistema estuarino, transfiriendo energía hacia los niveles tróficos más altos y contribuyendo a la pesca artesanal e industrial. El objetivo del artículo es evaluar la diversidad y abundancia de la fauna carcinológica de la región de Saco dos Limões y sus cambios bajo la influencia de las actividades de dragado durante la construcción de la carretera Bahía sur, Florianópolis, Santa

  2. Determinación del flujo de agua para la biorremediación en sistemas recirculados acuaculturales utilizando tapetes microbianos construidos

    OpenAIRE

    Jiménez-Montealegre,Ricardo; Zamora-Castro,Jorge; Zúñiga-Calero,Gerardo

    2015-01-01

    Se evaluó el efecto del flujo de agua sobre la eficiencia de tapetes microbianos para remover nitrógeno amoniacal total (NAT, N-NH4+ + N-NH3), nitritos (N-NO2-), nitratos (N-NO3-) y ortofosfatos (P-PO4-3) en un sistema de recirculación piloto para el cultivo de camarón blanco del Pacífico, Litopenaeus vannamei. Se evaluaron cuatro flujos de agua durante un período de 40 días: 5,2 ± 0,3 (A), 6,6 ± 1,3 (B), 8,4 ± 0,3 (C) y 12,2 ± 0,7 (D) L min-1. Las concentraciones de NAT y nitritos fueron sig...

  3. The invasion of five alien species in the Delta do Parnaíba Environmental Protection Area, Northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Daniel Loebmann

    2010-09-01

    Full Text Available Marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. In the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species were detected during dives, shore searches and from the fisheries on the coast of the Delta do Parnaíba Environmental Protection Area, in the States of Piauí and Maranhão, Northeastern Brazil. The species were the bicolor purse-oyster Isognomon bicolor, the whiteleg shrimp Litopenaeus vannamei, the giant river prawn Macrobrachium rosenbergii, the Indo-Pacific swimming crab Charybdis hellerii and, the muzzled blenny Omobranchus punctatus. Ballast water (I. bicolor, C. hellerii, and O. punctatus and aquaculture activities (L. vannamei and M. rosenbergii in adjacent areas are the most likely vectors of introduction. All exotic species found have potential impact risks to the environment because they are able to compete against native species for resources (food and habitat. Isognomon bicolor share the same habitat and food items with the native bivalve species of mussels and barnacles. Litopenaeus vannamei share the same habitat and food items with the native penaeids such as the pinkspot shrimp Farfantepenaeus brasiliensis, the Southern brown shrimp Farfantepenaeus subtilis, and the Southern white shrimp Litopenaeus schmitti, and in the past few years L. vannamei was responsible for a viral epidemics in the cultivation tanks that could be transmitted to native penaeid shrimps. Charybdis hellerii is also able to cause impacts on the local fisheries as the species can decrease the populations of native portunid crabs which are commercialized in the studied region. Macrobrachium rosenbergii may be sharing natural resources with the Amazon River prawn Macrobrachium amazonicum. Omobranchus punctatus shares habit with the native

  4. Solid targets for production of radioisotopes with cyclotron; Blancos solidos para produccion de radioisotopos con ciclotron

    Energy Technology Data Exchange (ETDEWEB)

    Paredes G, L.; Balcazar G, M. [Instituto Nacional de Investigaciones Nucleares, Direccion de Investigacion Tecnologica, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    The design of targets for production of radioisotopes and radiopharmaceuticals of cyclotron to medical applications requires a detailed analysis of several variables such as: cyclotron operation conditions, choice of used materials as target and their physicochemical characteristics, activity calculation, the yielding of each radioisotope by irradiation, the competition of nuclear reactions in function of the projectiles energy and the collision processes amongst others. The objective of this work is to determine the equations for the calculation for yielding of solid targets at the end of the proton irradiation. (Author)

  5. Degradation of a peptide in pitcher fluid of the carnivorous plant Nepenthes alata Blanco.

    Science.gov (United States)

    An, Chung-Il; Takekawa, Shoji; Okazawa, Atsushi; Fukusaki, Ei-Ichiro; Kobayashi, Akio

    2002-07-01

    Carnivorous plants acquire substantial amounts of nitrogen from insects. The tropical carnivorous plant Nepenthes produces trapping organs called pitchers at the tips of tendrils elongated from leaf ends. Acidic fluid is secreted at the bottoms of the pitchers. The pitcher fluid includes several hydrolytic enzymes, and some, such as aspartic proteinase, are thought to be involved in nitrogen acquisition from insect proteins. To understand the nitrogen-acquisition process, it is essential to identify the protein-degradation products in the pitcher fluid. To gain insight into protein degradation in pitcher fluid, we used the oxidized B-chain of bovine insulin as a model substrate, and its degradation by the pitcher fluid of N. alata was investigated using liquid chromatography-mass spectrometry (LC-MS). LC-MS analysis of the degradation products revealed that the oxidized B-chain of bovine insulin was initially cleaved at aromatic amino acids such as phenylalanine and tyrosine. These cleavage sites are similar to those of aspartic proteinases from other plants and animals. The presence of a series of peptide fragments as degradation products suggests that exopeptidase(s) is also present in the pitcher fluid. Amino acid analysis and peptide fragment analysis of the degradation products demonstrated that three amino acids plus small peptides were released from the oxidized B-chain of bovine insulin, suggesting that insect proteins are readily degraded to small peptides and amino acids in the pitcher fluid of N. alata.

  6. Physicochemical properties of functional surfaces in pitchers of the carnivorous plant Nepenthes alata Blanco (Nepenthaceae).

    Science.gov (United States)

    Gorb, E V; Gorb, S N

    2006-11-01

    Pitchers of the carnivorous plant Nepenthes alata are highly specialized organs adapted to attract, capture, and digest animals, mostly insects. They consist of several well distinguishable zones, differing in macro-morphology, surface microstructure, and functions. Since physicochemical properties of these surfaces may influence insect adhesion, we measured contact angles of non-polar (diiodomethane) and polar liquids (water and ethylene glycol) and estimated the free surface energy of 1) the lid, 2) the peristome, 3) the waxy surface of the slippery zone, and 4) the glandular surface of the digestive zone in N. alata pitchers. As a control, the external surface of the pitcher, as well as abaxial and adaxial surfaces of the leaf blade, was measured. Both leaf surfaces, both lid surfaces, and the external pitcher surface showed similar contact angles and had rather high values of surface free energy with relatively high dispersion component. These surfaces are considered to support strong adhesion forces based on the capillary interaction, and by this, to promote successful attachment of insects. The waxy surface is almost unwettable, has extremely low surface energy, and therefore, must essentially decrease insect adhesion. Both the peristome and glandular surfaces are wetted readily with both non-polar and polar liquids and have very high surface energy with a predominating polar component. These properties result in the preclusion of insect adhesion due to the hydrophilic lubricating film covering the surfaces. The obtained results support field observations and laboratory experiments of previous authors that demonstrated the possible role of different pitcher surfaces in insect trapping and retention.

  7. Aspartic proteinases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco.

    Science.gov (United States)

    An, Chung-Il; Fukusaki, Ei-ichiro; Kobayashi, Akio

    2002-03-01

    Carnivorous plants acquire significant amounts of nitrogen from insects. The tropical carnivorous plant Nepenthes accumulates acidic fluid containing aspartic proteinase (AP) in its trapping organs (pitchers), suggesting that the plant utilizes insect protein as a nitrogen source. Aspartic proteinases have been purified and characterized from sterile pitcher fluid of several species of Nepenthes; however, there is, as of yet, no information about sequence and expression of Nepenthes AP genes. To identify the pitcher AP, we cloned plant AP homologs from N. alata and examined their expressions. Five AP homologs ( NaAP1-NaAP5) were obtained by reverse transcription-polymerase chain reaction with degenerate primers designed for the conserved sequences of plant APs. Alignment of deduced amino acid sequences with other plant APs demonstrated that NaAP1-NaAP4 contained a plant-specific insert (PSI), a unique sequence of plant AP. However, NaAP5 did not possess the insert, and had a shorter sequence (by >100 amino acids) than the other APs. Northern analysis using a part of the coding region of NaAP1 as a probe showed that bands of approx. 1.8 kb corresponding to the sizes of NaAP1-NaAP4 mRNA were present in roots, stems, leaves, tendrils, and lower part of the pitchers, but a band of approx. 1.3 kb corresponding to the size of NaAP5 mRNA was not observed in any organs. In pitchers, highest expressions of NaAP1-NaAP4 were seen in the lower part of open pitchers containing natural prey, suggesting that the expressions of NaAP1-NaAP4 are coupled with prey capture. Transcripts of NaAP2 and NaAP4 were detected in the digestive glands, where AP secretion may occur. This result suggests that NaAP2 and NaAP4 are the possible APs secreted into the pitcher of N. alata.

  8. GERENCIA AVANZADA: UN BLANCO CADA VEZ MÁS MÓVIL

    Directory of Open Access Journals (Sweden)

    Luis Armando Gutiérrez Larrisgoitia

    2005-11-01

    Full Text Available El artículo desarrolla la prospectiva de las herramientas y modas gerenciales más conspicuas de los últimos cincuenta años, para luego caracterizar cualitativamente dichas prácticas según su impacto alrededor de tres dominios principales de la acción gerencial: la información, la tecnología, los recursos humanos, y sus combinaciones; el análisis subsiguiente permite hacer inferencias sobre cuánto de la “gerencia avanzada” es proactivo y cuanto es reactivo. El objetivo es hacer un pronóstico sobre las características que compartirán muchas prácticas gerenciales en el S.XXI, a partir de los atributos que las distingan, y del grado de congruencia con los entornos que exhiban.

  9. Demonstration of massive hydraulic fracturing Piceance Basin, Rio Blanco County, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Fitch, J L; Medlin, W L; Strubhar, M K

    1979-08-01

    Demonstration of massive fracturing to provide gas production from tight gas sands in the Piceance Basin was the objective of this jointly funded Mobil DOE project. This effort has been at least partially successful. The uppermost interval fractured, the Ohio Creek formation at 7324 to 7476 ft, appears to be commercially viable. The remaining sequence to total depth of 10,800 ft may also be commercially attractive, depending on fractured well costs, gas prices and the risk of failure to achieve production capacity equal to, or greater than, that achieved in the present well. Prior work was performed by Mobil in the Brush Creek Unit, Mesa County, Colorado. One well, Brush Creek 1-25, was drilled to 10,330 ft and given two massive fracturing treatments before the well was plugged and abandoned as noncommercial. It was concluded that formation permeability was too low to justify additional work in the Brush Creek Unit. Piceance Creek well F31-13G was drilled to 10,800 ft. Nine zones were tested in the Mesaverde and Ohio Creek formations between 7324 to 10,680 ft. Six massive fracturing treatments were performed covering 7 of the 9 intervals. Average first-year flow potential of the well is estimated at 2.9 MMCF/day with 1.1 MMCF/day of this amount attributed to the uppermost zone.

  10. El nuevo oro blanco: una mirada geopolítica desde América Latina

    OpenAIRE

    Acuña, Rosario; Acuña, Javier Alejandro; Aubert, Simón Jan; Cusatti, Juan Mariano

    2017-01-01

    La presente ponencia surge a partir de un trabajo de investigación para la materia de Geografía Económica Mundial de la carrera de Licenciatura en Geografía. En base a dicho trabajo y dado el interés que generó la temática es que junto a estudiantes de las carreras de Trabajo Social y de Comunicación Social de la Universidad Nacional de la Plata se decidió trabajar interdisciplinariamente para seguir profundizando sobre el litio y su implicancia en la geopolítica actual. El litio en los últim...

  11. Weathering of the Rio Blanco Quartz Diorite, Luquillo Mountains, Puerto Rico: Coupling Oxidation, Dissolution, And Fracturing

    International Nuclear Information System (INIS)

    Buss, H.L.; Sak, P.B.; Webb, S.M.; Brantley, S.L.

    2008-01-01

    In the mountainous Rio Icacos watershed in northeastern Puerto Rico, quartz diorite bedrock weathers spheroidally, producing a 0.2-2 m thick zone of partially weathered rock layers (∼2.5 cm thickness each) called rindlets, which form concentric layers around corestones. Spheroidal fracturing has been modeled to occur when a weathering reaction with a positive ΔV of reaction builds up elastic strain energy. The rates of spheroidal fracturing and saprolite formation are therefore controlled by the rate of the weathering reaction. Chemical, petrographic, and spectroscopic evidence demonstrates that biotite oxidation is the most likely fracture-inducing reaction. This reaction occurs with an expansion in d (0 0 1) from 10.0 to 10.5 (angstrom), forming 'altered biotite'. Progressive biotite oxidation across the rindlet zone was inferred from thin sections and gradients in K and Fe(II). Using the gradient in Fe(II) and constraints based on cosmogenic age dates, we calculated a biotite oxidation reaction rate of 8.2 x 10 -14 mol biotite m -2 s -1 . Biotite oxidation was documented within the bedrock corestone by synchrotron X-ray microprobe fluorescence imaging and XANES. X-ray microprobe images of Fe(II) and Fe(III) at 2 (micro)m resolution revealed that oxidized zones within individual biotite crystals are the first evidence of alteration of the otherwise unaltered corestone. Fluids entering along fractures lead to the dissolution of plagioclase within the rindlet zone. Within 7 cm surrounding the rindlet-saprolite interface, hornblende dissolves to completion at a rate of 6.3 x 10 -13 mol hornblende m -2 s -1 : the fastest reported rate of hornblende weathering in the field. This rate is consistent with laboratory-derived hornblende dissolution rates. By revealing the coupling of these mineral weathering reactions to fracturing and porosity formation we are able to describe the process by which the quartz diorite bedrock disaggregates and forms saprolite. In the corestone, biotite oxidation induces spheroidal fracturing, facilitating the influx of fluids that react with other minerals, dissolving plagioclase and chlorite, creating additional porosity, and eventually dissolving hornblende and precipitating secondary minerals. The thickness of the resultant saprolite is maintained at steady state by a positive feedback between the denudation rate and the weathering advance rate driven by the concentration of pore water O 2 at the bedrock-saprolite interface.

  12. Separation of a coastal upwelling jet at Cape Blanco, Oregon, USA ...

    African Journals Online (AJOL)

    This flow-topography interaction mechanism is a universal phenomenon, and is likely to be important in other eastern boundary current regions of the world. ... In 1994, cyclogenesis was observed, during which the coastal jet was connected with a cyclonic eddy offshore before the connection was severed and the jet again ...

  13. Rivera Blanco, Javier. (2008). De varia restauratione. 250 p. ISBN: 978-8496775275. Abada Editores, Barcelona.

    OpenAIRE

    Paris Fernández Gilbón

    2016-01-01

    El concepto “restauración” es relativamente nuevo (en la Alta Edad Media, al desaparecer las razones ideológicas y religiosas que valoraban los templos paganos “desapareció” el concepto) y al igual que su contexto socio histórico el comprenderlos requiere rastrear momentos clave de la historia de la humanidad, pues son en ellos donde se comprenden las implicaciones legales actuales, así como los derechos y obligaciones que nosotros como ciudadanos compartimos con el “académico viajero”. Para ...

  14. Rivera Blanco, Javier. (2008. De varia restauratione. 250 p. ISBN: 978-8496775275. Abada Editores, Barcelona.

    Directory of Open Access Journals (Sweden)

    Paris Fernández Gilbón

    2016-07-01

    Full Text Available El concepto “restauración” es relativamente nuevo (en la Alta Edad Media, al desaparecer las razones ideológicas y religiosas que valoraban los templos paganos “desapareció” el concepto y al igual que su contexto socio histórico el comprenderlos requiere rastrear momentos clave de la historia de la humanidad, pues son en ellos donde se comprenden las implicaciones legales actuales, así como los derechos y obligaciones que nosotros como ciudadanos compartimos con el “académico viajero”. Para llevar a buen fin dicho propósito, el autor nos expone un libro que incorpora una visión historicista de los sucesos más significativos de los siglos XVII al XX acompañados de una reflexión crítica de los conceptos de patrimonio y el papel de la academia en la restauración, rehabilitación, preservación y selección del Patrimonio; para finalizar con la “Carta de Cracovia” que es el documento internacional más reciente sobre el tema.

  15. The effect of 60Co γ irradiation on culture in vitro of Pogostemon cablin (blanco) benth

    International Nuclear Information System (INIS)

    Wu Lirong; He Hong; Zhang Yanling; Liu Xing; Lin Xiaohua

    2008-01-01

    The 60 Co γ-irradiation on culture in vitro of Pogostemon cablin is studied to breed new variety. The explants used for 60 Co γ-irradiation were the leaf segments, nodular stem segments, stem segments and root tips from Pogostemon cablin. The media for culture in vitro were MT basal media containing 0.05mg/L BA. As irradiation dosage increased, the mortality of different explants from Pogostemon cablin increased. The irradiation dosage of LD50 for leaf segments, nodular stem segments and stem segments were 72, 64 and 66Gy, respectively, which were deduced from the regression equation. As the irradiation dosage increased, the ability of the plant regeneration of the explants decreased. 60 Co γ-irradiation could make the explants damage or die. Irradiation could inhibit the regeneration of explants from Pogostemon cablin obviously. The changes in the appearance of the regenerated plants were also observed. (authors)

  16. A proposal for Rio Blanco region. Case scenarios for training and group discussion.

    Science.gov (United States)

    1993-01-01

    This supplement to "The Family Planning Manager" presents a case example and four related discussion questions to advance administrative staff's ability to prepare a pilot project proposal and budget. In the hypothetical scenario, the Ministry of Health has requested proposals for projects from each region that would increase contraceptive prevalence and reduce discontinuation by a national average of 15%. Outlined, in this document, is a two-year project that would use outreach workers to visit the homes of recent discontinuers and attempt to understand their concerns. The goal was to improve the health of mothers and children in the region by reducing the discontinuation rate among first-time users of contraceptives, while the objective was to reduce the discontinuation rate among first-time users from 27% to 15% by the end of the project. Also developed is a budget for the first year of the project that itemizes expenditures for salaries and wages, benefits, fees, general administration, travel and associated expenses, supplies and equipment, purchased services, and education and training. Finally, the supplement presents a sample summary sheet for the proposed project.

  17. El relato de las noticias sobre delito de cuello blanco. La criminalidad de etiqueta

    Directory of Open Access Journals (Sweden)

    Flavia Ivana Ragagnin

    2005-01-01

    Full Text Available A partir de las teorías de la comunicación, estudios sobre periodismo, la sociología jurídica, la criminología crítica y las teorías del etiquetamiento, la autora revela cómo desde el relato de los medios de comunicación el tratamiento de la información del delito en la clase social alta es diferente con respecto a la criminalidad común. La violencia simbólica que se sustrae del relato es indicio de esta diferenciación y se torna peligrosa para las instituciones democráticas al normalizar la violencia anómica, la ineficacia judicial y la impunidad.

  18. Social innovation and polycentric governance: The case of Juan Castro Blanco National Water Park

    NARCIS (Netherlands)

    Castro Arce, Karina; Parra, Constanza; Vanclay, Francis

    2015-01-01

    We focus on the role of social innovation in the governance of water-related challenges. We argue that in social-ecological challenges freshwater governance is improved by better understanding the dynamics of social innovation, specifically by analyzing the emergence of polycentric governance

  19. Sugar Daddy: Gilberto Freyre y el amor entre blancos y negros

    OpenAIRE

    Braga-Pinto, César

    2017-01-01

    Este ensayo se enfoca en las representaciones de la homosexualidad en el trabajo de Gilberto Freyre (1900-1987), particularmente, en la manera en que tales figuraciones alteran las nociones de contacto racial y mestizaje. Al echar una mirada a la configuración de los “deseos homosociales” en algunos de los textos de Freyre, se puede entender el rol que las narrativas de relaciones interraciales y homosexuales desempeñan en la estructuración de lo que él llama “la familia patriarcal brasilera”...

  20. Transferencias condicionadas e igualdad de género: ¿blancos, negros o grises?

    NARCIS (Netherlands)

    K. Voorend (Koen); J.M. Franzoni

    2008-01-01

    textabstract¿En qué medida las transferencias condicionadas (TMC), programas “estrella” de la nueva generación de la política social latinoamericana, inhiben o promueven mayores grados de igualdad de género? Usando un instrumento teórico-empírico novedoso, y a partir de fuentes primarias y

  1. Memorias del contacto. Relaciones indios-blancos en Misiones 1920-1960. 16H290

    OpenAIRE

    Gorosito Kramer, Ana María, dir.; Cabolla Badie, María Victoria; Gallero, María Cecilia

    2009-01-01

    En el presente trabajo de investigación nos proponemos incursionar en un tema poco analizado en la historia de Misiones: el contexto y la forma en que se produjeron los primeros contactos entre inmigrantes de origen europeo e indígenas mbya en la provincia en el periodo correspondiente a 1920-1960. Nos interesa también conocer cómo eran las relaciones entre los mbya y la población de origen paraguayo y criolla en general, quienes trabajaban como mano ...

  2. Hemo-oxigenasa 1: un promisorio blanco terapéutico

    OpenAIRE

    Sánchez, Carlos; Rodeiro, Idania; Garrido-Garrido, Gabino; Delgado, René

    2005-01-01

    La enzima hemo-oxigenasa es la principal enzima implicada en el catabolismo del grupo hemo y da lugar a tres productos fundamentales: biliverdina, el hierro libre y el monóxido de carbono. Fue descubierta a principios de la década del 60, pero no fue hasta mediado de los años ´80 donde empezó a estudiarse con detenimiento y se determinó que existía una isoforma inducible, denominada hemo oxigenasa-1. Esta proteína juega un papel muy importante en la modulación de procesos inflamatorios y eso ...

  3. Embryogenic callus formation in Dioscorea rotundata Poir cv. `Blanco de Guinea'

    Directory of Open Access Journals (Sweden)

    Dayana Rodríguez González

    2014-07-01

    Full Text Available Yam contributes to energy and nutritional requirements of most of the population of developing countries. However, their extensive culture is constrained by the limited availability of planting material with physiological and sanitary quality, and also part of the harvesting is used as seed in the next planting. For this reason, it is necessary to establish a methodology for plant regeneration and somatic embryogenesis could facilitate their micropropagation and genetic improvement. This study aimed to form embryogenic callus in Dioscorea rotundata Poir cv. `White Guinea'. The effect of the addition of 2,4-dichlorophenoxyacetic acid (2,4-D (0, 1.0, 2.0 and 4.0 mg l-1 was determined, in combination with three types of explants from in vitro plants (leaves petiole, petiole segments and root sections. The highest percentage of embryogenic callus was obtained with 1.0 mg l-1 2,4-D and leaves with petiole as explants. These were characterized by the presence of compact whitish nodules. Key words: 2,4-dichlorophenoxyacetic acid, somatic embryogenesis, micropropagation, yam

  4. Itinerari d’esilio, nel nome del Padre. Desde los blancos manicomios di Margarita Mateo Palmer

    OpenAIRE

    Scarabelli, Laura

    2014-01-01

    These are the signs that mark Margarita Mateo Palmer’s novel. This writing from the asylum challenges the preludes to immobility evoked by a place of order and restraint, thus enabling the emergence of a series of paths, which manage to shake the broken identity of the protagonist, Gelsomina, and of her time. Those intersections allow reflection on the contemporary Cuban condition, bent by the twilight of the revolutionary ideals, and at the same time to wonder about the human condition, sava...

  5. The correct name for Archidendron clypearia (Jack) Nielsen var. casai (Blanco) Nielsen (Leguminosae-Mimosoideae)

    NARCIS (Netherlands)

    Veldkamp, J.F.

    1997-01-01

    Recently Gangopadhyay & Chakrabarty (1993, 1994) proposed the combination Archidendron clypearia (Jack) Nielsen var. montanum (Benth.) Gang. & T. Chakrab. When I compared this to some papers Nielsen wrote (1984 (et al.); 1992) it would seem that this new combination would be identical with A.

  6. Effects of pesticides on DNA and protein of shrimp larvae Litopenaeus stylirostris of the California Gulf.

    Science.gov (United States)

    Galindo Reyes, J Guillermo; Leyva, Nancy R; Millan, Olivia A; Lazcano, Guadalupe A

    2002-10-01

    Recently, diverse pathologies and massive mortalities have been presented in shrimp hatcheries located along the California Gulf; therefore, toxic responses of shrimp larvae were used as biomarkers of pesticide pollution, because in this region intensive agriculture is practiced. Shrimp larvae were exposed to DDT, azinphosmethyl, permethrine, parathion, chlorpyrifos, malathion, endosulfan, and carbaryl, in order to determine LC50, DNA adducts and/or breaks, and total protein in larvae. The results indicate reductions in protein and DNA in larvae exposed to these pesticides, and in those exposed to DDT, breaks and/or adducts were registered. It is possible that pesticide pollution is a cause of these problems, because reduction in protein indicates a decrease in larvae growth rate and DNA breaks or adducts have been related to pathologies and carcinogenesis in many aquatic organisms.

  7. Pulsed ultraviolet light reduces immunoglobulin E binding to atlantic white shrimp (litopenaeus setiferus).

    Science.gov (United States)

    To date, the only effective method to prevent allergic reactions to shellfish is complete avoidance; however, if processing methods could be employed to minimize shellfish allergens before products reach consumers, illness could be substantially lessened. Pulsed ultraviolet light (PUV), a novel food...

  8. Physiochemical Changes and Optimization of Phosphate-Treated Shrimp (Litopenaeus vannamei ) Using Response Surface Methodology.

    Science.gov (United States)

    Omar, Saiah Djebbour; Yang, Je-Eun; Oh, Sang-Cheol; Kim, Dae-Wook; Lee, Yang-Bong

    2016-03-01

    The objective of this study was to determine the factors responsible for the changed physiochemical properties of unpeeled shrimp treated in cold phosphate solution (2~4°C) with the intervention of 4 factors: phosphate concentration, dipping time, rotation speed, and volume of brine solution. Response surface analysis was used to characterize the effect of the phosphate treatment on shrimps by running 33 treatments for optimizing the experiment. For each treatment, phosphate amount, moisture content, and weight gain were measured. The results showed that phosphate concentration is the most important factor than other factors for facilitating phosphate penetration in the meat of the shrimp and for getting the best result. The optimum condition of phosphate-treated shrimp in this study was 110 to 120 min dipping time, 500 to 550 mL brine solution for 100 g shrimp sample, and 190 to 210 rpm agitation speed. The studied conditions can be applied in fisheries and other food industries for good phosphate treatments.

  9. BUDI DAYA UDANG VANNAMEI (Litopenaeus vannamei POLA TRADISIONAL PLUS DI KABUPATEN MAROS, SULAWESI SELATAN

    Directory of Open Access Journals (Sweden)

    Erfan A. Hendarajat

    2007-12-01

    Full Text Available Udang merupakan salah satu komoditas perikanan unggulan dalam program revitalisasi perikanan, di samping rumput laut dan tuna. Pada awalnya, jenis udang yang dibudidayakan di air payau adalah udang windu, namun setelah mewabahnya penyakit terutama WSSV yang mengakibatkan menurunnya usaha budi daya udang windu, pemerintah kemudian mengintroduksi udang vannamei pada tahun 2001 untuk membangkitkan kembali usaha perudangan di Indonesia dan dalam rangka diversifikasi komoditas perikanan. Untuk mengembangkan budi daya udang kedepan, upaya-upaya yang dilakukan pemerintah antara lain: (i Revitalisasi tambak intensif dengan udang vannamei seluas 7.000 ha dengan produktivitas 30 ton/ha/tahun, (ii revitalisasi tambak tradisional seluas 140.000 ha (40% dari tambak tradisional dengan produktivitas 600--700 kg/ha/tahun, (iii impor vannamei SPF/SPR, (iv pengembangan induk SPF vannamei dalam negeri, (v revitalisasi backyard hatchery  (HSRT, (vi penerapan sertifikasi, (vii pengembangan laboratorium, dan (viii pengembangan sarana/prasarana (Nurjana, 2005. Aspek-aspek yang perlu diperhatikan dalam usaha budi daya udang vannamei pola tradisional plus antara lain: persiapan tambak, kualitas benih, teknik penebaran, padat penebaran, manajemen pakan, pemeliharaan kualitas air, dan teknik panen. Tulisan ini menjelaskan secara ringkas mengenai teknologi budi daya udang vannamei pola tradisional plus di Kabupaten Maros, Sulawesi Selatan yang dapat dilakukan oleh pembudi daya udang.

  10. Isolation and Structural Elucidation of Antiproliferative Compounds of Lipidic Fractions from White Shrimp Muscle (Litopenaeus vannamei)

    Science.gov (United States)

    López-Saiz, Carmen-María; Velázquez, Carlos; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2014-01-01

    Shrimp is one of the most popular seafood items worldwide, and has been reported as a source of chemopreventive compounds. In this study, shrimp lipids were separated by solvent partition and further fractionated by semi-preparative RP-HPLC and finally by open column chromatography in order to obtain isolated antiproliferative compounds. Antiproliferative activity was assessed by inhibition of M12.C3.F6 murine cell growth using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay. The methanolic fraction showed the highest antiproliferative activity; this fraction was separated into 15 different sub-fractions (M1–M15). Fractions M8, M9, M10, M12, and M13 were antiproliferative at 100 µg/mL and they were further tested at lower concentrations. Fractions M12 and M13 exerted the highest growth inhibition with an IC50 of 19.5 ± 8.6 and 34.9 ± 7.3 µg/mL, respectively. Fraction M12 was further fractionated in three sub-fractions M12a, M12b, and M12c. Fraction M12a was identified as di-ethyl-hexyl-phthalate, fraction M12b as a triglyceride substituted by at least two fatty acids (predominantly oleic acid accompanied with eicosapentaenoic acid) and fraction M12c as another triglyceride substituted with eicosapentaenoic acid and saturated fatty acids. Bioactive triglyceride contained in M12c exerted the highest antiproliferative activity with an IC50 of 11.33 ± 5.6 µg/mL. Biological activity in shrimp had been previously attributed to astaxanthin; this study demonstrated that polyunsaturated fatty acids are the main compounds responsible for antiproliferative activity. PMID:25526568

  11. Selection of proteolytic bacteria with ability to inhibit Vibrio harveyi during white shrimp (Litopenaeus vannamei cultivation

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    Suntinanalert, P.

    2007-03-01

    Full Text Available Five isolates of bacteria with high proteolytic activity, isolated from water samples of intensive shrimp ponds in southern Thailand, were selected to test for the ability to control the shrimp pathogen Vibrioharveyi. 70 μl of each culture broth were investigated for their ability to inhibit V. harveyi using an agar well diffusion test but only one isolate W3 gave a reasonable sized inhibition zone of 21.62 mm. This zone wassimilar to that of oxolinic acid (2 μg and sulfamethoxazole (25 μg. The W3 isolate was identified as Pseudomonas sp. Shrimp cultivation in aquaria was conducted to investigate the inhibition of V. harveyi bythe isolate W3. The experiment consisted of a treatment of the shrimp culture with an inoculum of the isolate W3 and V. harveyi (biocontrol set, a positive control set (only inoculation of V. harveyi and a negativecontrol set as without inoculation. No mortality was found in the negative control. Shrimp mortality in the biocontrol set (33% was lower than that in the positive control set (40%; however, it showed no significantdifference (p>0.05. The average numbers of V. harveyi over 12 days of the biocontrol set were lower than those in the positive control set by about 1 log cycle although the numbers were not significantly different(p>0.05. The shrimp growth rate at day 32 of cultivation was in order of the biocontrol treatment (10.17% > the negative control treatment (9.44% > the positive control set (9.28%, but no significant difference (p>0.05 was observed among treatments.

  12. Biofiltration, growth and body composition of oyster Crassostrea rhizophorae in effluents from shrimp Litopenaeus vannamei1

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    Rafael Vieira de Azevedo

    Full Text Available The objective of this study was to use oyster as biofilter to improve the quality of effluent from shrimp farming and to assess its growth performance and body composition. It was distributed 1,080 oysters into lanterns in fiberglass tanks (170 L in a completely randomized design with three treatments (0, 60 and 120 oysters and six replicates. It was used the effluent from the sedimentation tank. It was measured weekly: temperature, salinity, dissolved oxygen and pH, and it was analyzed ammonia-N, nitrite-N, nitrate-N, orthophosphate-P, suspended solids and chlorophyll-α of the input effluent. The control tanks (without oysters were more efficient at removing ammonia-N, nitrite-N, nitrate-N and orthophosphate-P. The tanks containing oysters were more efficient at removing suspended solids and chlorophyll-α. Stocking density influenced the height growth of oysters, but not width. Wet and daily weight, condition and yield index were not affected by stocking density, and a significant increase in comparison to the initials values was observed. Body composition was not affected by stocking density, and a significant difference (p0.05. Under the conditions evaluated, the oyster Crassostrea rhizophorae improves water quality and presents growth rates and body composition similar to those obtained in traditional crops.

  13. Preparation and Characterization of Chitosan Obtained from Shells of Shrimp (Litopenaeus vannamei Boone).

    Science.gov (United States)

    de Queiroz Antonino, Rayane Santa Cruz Martins; Lia Fook, Bianca Rosa Paschoal; de Oliveira Lima, Vítor Alexandre; de Farias Rached, Raid Ícaro; Lima, Eunice Paloma Nascimento; da Silva Lima, Rodrigo José; Peniche Covas, Carlos Andrés; Lia Fook, Marcus Vinícius

    2017-05-15

    The main source of commercial chitosan is the extensive deacetylation of its parent polymer chitin. It is present in green algae, the cell walls or fungi and in the exoskeleton of crustaceans. A novel procedure for preparing chitosan from shrimp shells was developed. The procedure involves two 10-minutes bleaching steps with ethanol after the usual demineralization and deproteinization processes. Before deacetylation, chitin was immersed in 12.5 M NaOH, cooled down and kept frozen for 24 h. The obtained chitosan was characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), UV, X-ray diffraction (XRD) and viscosimetry. Samples of white chitosan with acetylation degrees below 9 % were obtained, as determined by FTIR and UV-first derivative spectroscopy. The change in the morphology of samples was followed by SEM. The ash content of chitosan samples were all below 0.063 % . Chitosan was soluble in 1 % acetic acid with insoluble contents of 0.62 % or less. XRD patterns exhibited the characteristic peaks of chitosan centered at 10 and 20 degrees in 2 θ . The molecular weight of chitosan was between 2.3 and 2.8 × 10 5 g/mol. It is concluded that the procedure developed in the present work allowed obtaining chitosans with physical and chemical properties suitable for pharmaceutical applications.

  14. Preparation and Characterization of Chitosan Obtained from Shells of Shrimp (Litopenaeus vannamei Boone

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    Rayane Santa Cruz Martins de Queiroz Antonino

    2017-05-01

    Full Text Available The main source of commercial chitosan is the extensive deacetylation of its parent polymer chitin. It is present in green algae, the cell walls or fungi and in the exoskeleton of crustaceans. A novel procedure for preparing chitosan from shrimp shells was developed. The procedure involves two 10-minutes bleaching steps with ethanol after the usual demineralization and deproteinization processes. Before deacetylation, chitin was immersed in 12.5 M NaOH, cooled down and kept frozen for 24 h. The obtained chitosan was characterized using scanning electron microscopy (SEM, Fourier transform infrared spectroscopy (FTIR, UV, X-ray diffraction (XRD and viscosimetry. Samples of white chitosan with acetylation degrees below 9 % were obtained, as determined by FTIR and UV-first derivative spectroscopy. The change in the morphology of samples was followed by SEM. The ash content of chitosan samples were all below 0.063 % . Chitosan was soluble in 1 % acetic acid with insoluble contents of 0.62 % or less. XRD patterns exhibited the characteristic peaks of chitosan centered at 10 and 20 degrees in 2 θ . The molecular weight of chitosan was between 2.3 and 2.8 × 10 5 g/mol. It is concluded that the procedure developed in the present work allowed obtaining chitosans with physical and chemical properties suitable for pharmaceutical applications.

  15. Potensi sinbiotik lokal terhadap respon imun non spesifik udang vaname (Litopenaeus vannamei

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    Indri Saputri Ramadhani

    2017-09-01

    Full Text Available The aims of the study were to examine the exact percentage of prebiotics in sinbiotics, administered through feed, so as to enhance nonspecific immune responses in white shrimp. The sweet potato extract was used as prebiotic, combined with local isolated Bacillus sp. D2.2 as a probiotic applied simultaneously as a synbiotic. The feed was treated with 0% sinbiotic treatment (treatment A / control, 0% prebiotic and 6% probiotics (B treatment, 2% prebiotic and 6% probiotics (treatment C, 4% prebiotics and 6% probiotics (treatment D. Examination of nonspecific immune responses to shrimp included total hemocyte count (THC, phagocytosis activity (AP, phagocytosis index (IP, phenoloxidase activity (PO, and superoxide dismutase activity (SOD. Observation of the non-specific immune response of vaname shrimp after treatment showed that treatment C was the best prebiotic  to increase non-specific immune response in vaname shrimp. Penelitian ini bertujuan untuk mempelajari persentase prebiotik yang tepat dalam sinbiotik, yang diberikan melalui pakan, sehingga dapat meningkatkan respon imun nonspesifik pada udang vaname. Dalam penelitian ini digunakan ekstrak tepung ubi jalar sebagai prebiotik, dikombinasikan dengan isolat lokal Bacillus sp. D2.2 sebagai probiotik yang diaplikasikan secara bersamaan sebagai sinbiotik. Pakan yang diberikan adalah pakan dengan perlakuan 0% sinbiotik (perlakuan A/kontrol, 0% prebiotik dan 6% probiotik (perlakuan B, 2% prebiotik dan 6% probiotik (perlakuan C, 4% prebiotik dan 6% probiotik (perlakuan D. Pemeriksaan respon imun nonspesifik pada udang meliputi total hemocyte count (THC, aktivitas fagositosis (AF, indeks fagositosis (IF, aktivitas phenoloxidase (PO, dan aktivitas superoxide dismutase (SOD. Pengamatan pada respon imun non spesifik udang vaname setelah diberi perlakuan menunjukan bahwa perlakuan C merupakan persentase prebiotik terbaik untuk meningkatkan respon imun non spesifik pada udang vaname.

  16. LA MEMBRANA PERITRÓFICA EN Prostephanus truncatus (HORN (COLEÓPTERA: BOSTRICHIDAE: ¿BLANCO PARA SU CONTROL? LA MEMBRANA PERITRÓFICA EN Prostephanus truncatus (HORN (COLEÓPTERA: BOSTRICHIDAE: ¿BLANCO PARA SU CONTROL?

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    M. Vázquez-Arista

    2012-02-01

    Full Text Available Prostephanus truncatus (Horn is a pest of maize stored in Mexico, Central America, some parts of South America, and it is expanding rapidly in Africa since it was introduced to that continent. Control of this pest in storehouses is carried out with the massive use of pesticides. With the object of finding an alternative method of control, an electron microscope study was made of the mid intestine of adults and larvae. Detected in the mid intestine of adult. P. truncatus was the presence of a peritrophic membrane. This membrane is formed by an irregular grid composed of various layers which are absent in the mid intestine of the last larval stage of P. truncatus. This indicates that formation of the peritrophic membrane take place mainly during the pupal stage, for which P. truncatus may be controlled biologically by using transgenic plants with the specific information to interfere in the synthesis of this membrane.Prostephanus truncatus (Horn es un insecto plaga del maíz almacenado en México, América Central, parte de América del Sur y se está expandiendo rápidamente en África desde que fue introducido a este continente. El control de este insecto en los almacenes se lleva a cabo con el uso masivo de insecticidas. Con el objeto de encontrar una alternativa para su control, se realizó un estudio de microscopía electrónica en el intestino medio del insecto adulto y su larva. Se detectó, en el intestino medio de P. truncatus adulto, la presencia de una membrana peritrófica. Esta membrana está formada por una red irregular compuesta de varias capas, la cual está ausente en el intestino medio del último estadío larvario de P. truncatus. Esto indica que su formación tiene lugar, principalmente, durante el período de pupa, por lo que P. truncatus podría ser controlado biológicamente usando plantas transgénicas con la información específica para interferir en la síntesis de esta membrana.

  17. Posvjashenije Eve / Mark Levin

    Index Scriptorium Estoniae

    Levin, Mark

    2000-01-01

    Kahest lavastusest : Vene Draamateatri monoetendusest Dario Fo/F.Rame "Ootan sind, kallim" Ljubov Agapovaga, lavastaja Irina Tomingas ja Moskva Vahtangovi nim. Teatri etendusest E. Schmitti "Pühendus Eevale", lavastaja Sergei Jashin

  18. Vaikne Ö taaselustus

    Index Scriptorium Estoniae

    2008-01-01

    15. augustil sai Võsu rannaklubis muuhulgas vaadata filmi "Nukitsamees" ja kuulata Rakvere linnanoorte teatritrupi noori lugemas Eric-Emmanuel Schmitt'i raamatut "Härra Ibrahim ja Koraani õied". Ürituse eestvedaja on Kaire Leemet

  19. The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco accessions

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    Coletta Filho Helvécio Della

    2000-01-01

    Full Text Available RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

  20. Plasma-membrane H+-ATPases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco.

    Science.gov (United States)

    An, C I; Fukusaki, E; Kobayashi, A

    2001-03-01

    Nepenthes is a unique genus of carnivorous plants that can capture insects in trapping organs called pitchers and digest them in pitcher fluid. The pitcher fluid includes digestive enzymes and is strongly acidic. We found that the fluid pH decreased when prey accumulates in the pitcher fluid of Nepenthes alata. The pH decrease may be important for prey digestion and the absorption of prey-derived nutrients. To identify the proton pump involved in the acidification of pitcher fluid, plant proton-pump homologs were cloned and their expressions were examined. In the lower part of pitchers with natural prey, expression of one putative plasma-membrane (PM) H+-ATPase gene, NaPHA3, was considerably higher than that of the putative vacuolar H+-ATPase (subunit A) gene, NaVHA1, or the putative vacuolar H+-pyrophosphatase gene, NaV-HP1. Expression of one PM H+-ATPase gene, Na-PHA1, was detected in the head cells of digestive glands in the lower part of pitchers, where proton extrusion may occur. Involvement of the PM H+-ATPase in the acidification of pitcher fluid was also supported by experiments with proton-pump modulators; vanadate inhibited proton extrusion from the inner surface of pitchers, whereas bafilomycin A1 did not, and fusicoccin induced proton extrusion. These results strongly suggest that the PM H+-ATPase is responsible for acidification of the pitcher fluid of Nepenthes.

  1. Editorial: Libro Blanco de la Ingeniería de Software en América Latina

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    Edgar Serna M.

    2014-06-01

    Full Text Available Mucho se ha dicho y escrito acerca de la Ingeniería de Software. Por desgracia, gran parte de esa producción se ha escrito desde la academia con una perspectiva que no siempre responde a las preocupaciones y necesidades de las partes interesadas e involucradas en los procesos del desarrollo de software. Debido al continuo incremento en la complicación de los problemas que se deben solucionar desde esta área del conocimiento, y con la dependencia cada vez mayor de la sociedad en la tecnología, se necesita cambiar la visión de esta ingeniería y pensarla como una profesión, y ver a sus practicantes como profesionales. Ese es en parte el objetivo de este libro, porque sus diferentes capítulos guían al lector por caminos de sensibilización y comprensión del papel de esta área en el siglo XXI.

  2. Use of an infrared thermometer for assessment of plant water stress in neck orange (Citrus reticulata Blanco

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    Sayan Sdoodee

    2006-11-01

    Full Text Available In general, water stress causes stomatal closure in citrus, and this leads to higher leaf temperature. Recently, it has been reported that infrared thermometry technique can be used for detecting stomatal closure indirectly to assess plant water stress. Therefore, it was proposed to apply to neck orange. An experiment was arranged as a completely randomized design. There were 3 treatments of watering levels: 1 wellwatering (W1, 2 3-day interval watering (W2, and 3 6-day interval watering (W3 with 6 replicates. Eighteen 2-year-old trees of neck orange were used, and each tree was grown in a container (0.4 m3 filled with mixed media of soil, compost and sand (1:1:1. During 18 days of the experimental period, it was found that leaf water potential and stomatal conductance of the plants in W2 and W3 treatments decreased with the progress of water stress. There was high correlation (r2 = 0.71** between leaf water potential and stomatal conductance as a linear regression (Y = 0.0044X-1.8635. Canopy temperature (Tc and air temperature (Ta of each tree were measured by an infrared thermometer, and Tc-Ta was assessed. At the end of the experimental period, it was found that Tc-Ta was significantly highest in the W3 treatment (3.5ºC followed by the of W2 treatment (2ºC, while it was lowest in the W1 treatment (1ºC. The relationship between Tc-Ta and stomatal conductance was high as polynomial (Y = 0.0002X2 0.0572X+3.9878, r2 = 0.70**. This indicated that stomatal closure or decreasing stomatal conductance caused increasing of Tc-Ta in the leaves. Hence, it suggests that infrared thermometer is a convenient device for the assessment of plant water stress in neck orange.

  3. Perbedaan respon Aedes aegypti (Linnaeus) (Diptera: Culicidae), terhadap paparan anti nyamuk bakar dan bunga keluwih (Artocarpus camansi, Blanco)

    OpenAIRE

    Nur Endah Wahyuningsih; Ramauli Agustina Sihit

    2015-01-01

    The control of dengue hemorrhagic fever (DHF) it is important to control the vector, i.e. Aedes aegypti (Linnaeus) and Ae. albopictus (Skuse). Usually, controls of these vectors are done using chemical insecticides. Research on mosquito resistance has been done, but the impact of mosquitoes that survive after synthetic (chemical) insecticides application has not been studied. The aim of this research was to analyze the differences of fecundity, fertility and vitality rate of mosquitoes that w...

  4. GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco (Moraceae leaves

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    Franelyne Pataueg Casuga

    2016-11-01

    Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.

  5. HISTORIA DE LAS INVESTIGACIONES RECIENTES EN LA CUEVA DE AMBROSIO (VÉLEZ-BLANCO, ALMERÍA, ESPAÑA

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    Sergio Ripoll López

    2013-11-01

    Full Text Available En 1982 iniciamos una nueva serie de excavaciones arqueológicas sistemáticas en La Cueva de Ambrosio que han continuado, con interrupciones, hasta el presente. Estos trabajos, primero con los permisos y soporte económico del Ministerio de Cultura, y posteriormente, con los de la Dirección General de Bienes Culturales de la Junta de Andalucía. Durante la campaña de 1992 realizamos el descubrimiento de arte rupestre cubierto por sedimentos arqueológicos. Cien años después de las excavaciones de Breuil y Motos y cincuenta años de las de E. Ripoll Perelló, el año 2012 marca el 30 aniversario del comienzo de nuestras excavaciones en este yacimiento.In 1982 we began a systematic series of campaigns that have continued with many interruptions to the present. First was the Ministry of Culture which took over the permissions and the grant of Dirección General de Bienes Culturales de la Junta de Andalucía. During the campaign of 1992 we made the discovery of rock art covered by archaeological sediments. In short, 100 years of excavations of Breuil and Motos, 50 years of those of E. Ripoll Perelló, 2012 also marks the 30th anniversary of our work at the site.

  6. Los "Acaritos Blancos" (Insecta: psocoptera): una plaga erróneamente interpretada en la industria de Costa Rica

    OpenAIRE

    Jirón Porras, Luis Fernando; Vargas V., Mario

    2001-01-01

    Se informa sobre la presencia por primera vez, como plaga en Costa Rica, del psócido Liposcelis spp. (Troctomorpha: Liposcelidae), Mockford, 1991. UCR::Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro Nacional de Ciencia y Tecnología de Alimentos (CITA)

  7. Desarrollo de un Modelo Matemático para la Fermentación de Cacao Criollo Blanco

    OpenAIRE

    López Monzon, Robinson

    2016-01-01

    Los granos de cacao (Thebroma cacao L.) son el principal material para la producción de chocolate. La fermentación de los granos de cacao es esencial para remover la pulpa (mucílago) que envuelve a los granos y para el desarrollo de los precursores químicos del sabor del chocolate. La ecología microbiana de la fermentación de los granos de cacao es compleja e involucra el crecimiento secuencial de varias especias de levaduras, bacterias ácido lácticas (LAB), bacterias ácido acéticas y posible...

  8. Caracterização da pesca artesanal no complexo estuarino-lagunar de Cananéia-Iguape, Estado de São Paulo, Brasil (1995-1996 Amateur fishery in the estuary-lagoon complex of Cananéia-Iguape, state of São Paulo, Brazil (1995-1996

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    Jocemar Tomasino Mendonça

    2001-05-01

    , currently in operation, is motor-powered. The activity is, however, important to the region and contributes with 10 to 32% of total landing in Cananéia. The local amateur fishery is divided into two main groups: the near-shore fishery and the estuarine fishery. The former mainly exploits the Atlantic seabob (Xiphopenaeus kroyeri, the white shrimp (Litopenaeus schmitti. Fishermen fish with gillnet or long line in a typical small boat bottom trawl called as “bateira”. The fishing gear and techniques of the local estuarine fishery include the fence trap, the gillnet, and the floating and vertical long line. A typical small drift beam trawl net, locally known as “gerival”, is also used to catch juvenile shrimp Farfantepenaeus paulensis and F. brasiliensis, highly valuable bait for recreational fishing. The fence trap is the main fishing gear and more than 90 traps were set inside the estuary, where the target species is mullet (Mugil platanus, although many other species are also important catch items, e.g. Mugil curema, snook (Centropomus spp., whitemouth croaker (Micropogonias furnieri, king weakfish (Macrodon ancylodon and mojarras (Diapterus rhombeus. The production of Atlantic seabob, the most important species of Cananéia’s amateur fishery, fluctuated from year to year. The amateur fishery landings of Atlantic seabob were 34 t. in 1995 and 12 t. in 1996

  9. Silagem ácida do resíduo do camarão Litopenaeus vannamei em rações para tilápia do Nilo = Acid silage of shrimp Litopenaeus vannamei waste in Nile tilapia diets

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    Carolina Nunes Costa

    2009-04-01

    Full Text Available Rações isoproteicas (30% PD e isoenergéticas (3.200 kcal kg-1 foramformuladas, utilizando-se a silagem ácida de resíduo de camarão branco como fonte proteica alternativa em cinco diferentes níveis de inclusão 0, 4, 8, 12 e 16%. Foram utilizados 200 peixes com peso médio de 7,2 ± 0,5 g, distribuídos aleatoriamente em 20 tanques de 100 L cada, numa densidade de dez peixes tanque-1, em sistema de recirculação de água fechado. Foi utilizado um delineamento inteiramente casualizado com cinco tratamentos e quatro repetições. Foram avaliados parâmetros de desempenho produtivo e composição centesimal do tecido muscular em alevinos de tilápia, alimentados três vezes por dia, até a saciedade aparente, durante 60 dias. Foram observados efeito quadrático (p Isoproteic (30% DP and isoenergetic (3,200 kcal kg-1 diets were formulated using acid silage of shrimp waste as alternative protein source at five levels (0, 4, 8, 12 and 16%. Two hundred fish (7.2 ± 0.5 g were randomly distributed in 20 tanks of 100 L at a density of10 fish tank-1 in a closed recirculation water system. The experiment was accomplished in a completely randomized design with 5 treatments and 4 replicates. Growth performance and proximal composition parameters of the muscular tissue were evaluated to tilapia fed threetimes a day to satiation during sixty days. Quadratic effect (p < 0.05 to weight gain, protein efficiency ratio, specific growth rate, protein retention, and linear effect (p < 0.05 to feed consumption, feed conversion rate and gross lipid in muscular tissue were observed. The acid silage of shrimp L. vannamei waste provide better growth performance, without losses in proximate composition of the muscular tissue, with 2.75% of inclusion in fingerling Niletilapia diets.

  10. Use of TPP and ATPS for partitioning and recovery of lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas.

    Science.gov (United States)

    Kuepethkaew, S; Sangkharak, K; Benjakul, S; Klomklao, S

    2017-11-01

    Lipase recovery from Pacific white shrimp hepatopancreas using a three-phase partitioning (TPP) system in combination with an aqueous two-phase system (ATPS) was studied. TPP system was formed with a simultaneous addition of salt directly to crude extract (CE) followed by an organic solvent addition. The various process parameters required for efficient purification of lipase were optimized. The best lipase yield (87.41%) and purification fold (PF) (3.49-fold) were obtained in the interphase of TPP system, which consisted of the CE to t -butanol ratio of 1:1 (v/v) in the presence of 50% (w/v) (NH 4 ) 2 SO 4 . Subsequently, TPP fraction was subjected to ATPS. Effects of phase compositions including PEG molecular weight and concentration, types and concentration of salts, NaCl addition and system pH on lipase partitioning were investigated. With the application of 25% (w/w) PEG1000 and 15% (w/w) MgSO 4 , at pH 5.0 was found most appropriate since high lipase PF (5.19-fold) and yield (78.46%) in top phase were obtained. The partitioned enzyme exhibited optimal activity at pH 8.0 and 55 °C and was stable at a temperature range of 0-40 °C and a pH range of 7-10. The partitioned lipase showed high tolerance in the presence of ethanol and methanol. Hence, the combined partitioning systems, TPP-ATPS, were found to be an attractive technique for the recovery and partial purification of lipase from Pacific white shrimp hepatopancreas.

  11. Polyculture Engineering technology of larasati red tilapia (Oreochromis niloticus) and white shrimp (Litopenaeus vannamei) based for protease enzyme

    Science.gov (United States)

    Samidjan, I.; Rachmawati, D.

    2018-04-01

    The objective is polyculture technology of red tilapia larasati fish and white shrimp with different combinations density. The material is saline red tilapia larasati 3.29 ± 0.018 g and white shrimp with initial weight 1.39 ± 0.025 g. Seeds are density of red tilapia larasati larvae 5 and 10 larvae / m2 fish. And white shrimp 5 larvae / m2 and 10 larvae / m2. An artificial feed used enzyme dose of 2.25 g / kg. The experimental using complete randomized design 4 treatments and 3 replications that is given seeds 5 larvae / m2 larvae red tilapia larasati and given 5 larvae / m2 white shrimp (A), 5 larvae / m2 red tilapia) and 10 m2 / m2 of white shrimp (B), 10 m2 larvae and 5 m2 white shrimp (C), 10 m2 larvae and 10 m2 white shrimp (D)). The data were growth of absolute weight, survival rate, FCR, and water quality data (temperature, salinity, pH, O2, NO2, NH3). Data were analyzed of variance (F test). The results showed significantly effect (P <0.01) on the growth. The highest absolute growth in D treatment were red tilapia larasati (185.75 ± 0.50g) and white shrimp (25.25 ± 0.95 g).

  12. Composition, diversity and function of intestinal microbiota in pacific white shrimp (Litopenaeus vannamei at different culture stages

    Directory of Open Access Journals (Sweden)

    Shenzheng Zeng

    2017-11-01

    Full Text Available Intestinal microbiota is an integral component of the host and plays important roles in host health. The pacific white shrimp is one of the most profitable aquaculture species commercialized in the world market with the largest production in shrimp consumption. Many studies revealed that the intestinal microbiota shifted significantly during host development in other aquaculture animals. In the present study, 22 shrimp samples were collected every 15 days from larval stage (15 day post-hatching, dph to adult stage (75 dph to investigate the intestinal microbiota at different culture stages by targeting the V4 region of 16S rRNA gene, and the microbial function prediction was conducted by PICRUSt. The operational taxonomic unit (OTU was assigned at 97% sequence identity. A total of 2,496 OTUs were obtained, ranging from 585 to 1,239 in each sample. Forty-three phyla were identified due to the classifiable sequence. The most abundant phyla were Proteobacteria, Cyanobacteria, Tenericutes, Fusobacteria, Firmicutes, Verrucomicrobia, Bacteroidetes, Planctomycetes, Actinobacteria and Chloroflexi. OTUs belonged to 289 genera and the most abundant genera were Candidatus_Xiphinematobacter, Propionigenium, Synechococcus, Shewanella and Cetobacterium. Fifty-nine OTUs were detected in all samples, which were considered as the major microbes in intestine of shrimp. The intestinal microbiota was enriched with functional potentials that were related to transporters, ABC transporters, DNA repair and recombination proteins, two component system, secretion system, bacterial motility proteins, purine metabolism and ribosome. All the results showed that the intestinal microbial composition, diversity and functions varied significantly at different culture stages, which indicated that shrimp intestinal microbiota depended on culture stages. These findings provided new evidence on intestinal microorganism microecology and greatly enhanced our understanding of stage-specific community in the shrimp intestinal ecosystem.

  13. Bioavailability of crude protein and lipid from biofloc meals produced in an activated sludge system for white shrimp, Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Hassan Sabry Neto

    2015-08-01

    Full Text Available The present study compared the bioavailability of crude protein and lipid from biofloc meals generated with an activated sludge system using two water sources: wastewater from shrimp experimental culture (BFL-W and, artificially, using clean seawater (BFL-C. The sludge system operated by chemical and organic fertilization three times per week. Sampling of bioflocs occurred every two days during 81 days. To evaluate digestibility, each type of biofloc meal was incorporated into a reference diet (REF at 300 g/kg. Another diet acted as a negative control (NEG by using fish waste meal. The apparent digestibility of bioflocs was estimated by the indirect method using chromic oxide (Cr2O3 as the inert marker at 10 g/kg of the diet. Juvenile L. vannamei of 5.09±0.79 g (n = 440 were stocked at 10 shrimp/tank in 44 tanks of 61 L each that operated under a water recirculating regime. Biofloc meals contained a high ash content (591.0-649.2 g/kg combined with a low crude protein content (95.9-137.3 g/kg. After 26 days, shrimp achieved a final survival of 93.2±0.8% and a biomass gain of 37.1±1.8 g/tank. Final shrimp body weight ranged from 9.01±0.15 to 9.45±0.13 g. The apparent digestibility coefficient (ADC of crude protein in the biofloc produced from BFL-W, BFL-C and fish waste meal (NEG reached 26.0, 25.7, and 64.1%, respectively. Similarly, the lipid ADC was 78.9, 67.9, and 85.8%, respectively. This study indicated that biofloc meals had a low protein availability for L. vannamei. However, although low levels of lipid were present, it proved to be available for the species. The dietary inclusion of biofloc meal appears to have a growth-promoting effect on shrimp, which may be associated with trace minerals, or other nutrients not identified in this study.

  14. THE EFFECT OF PROBIOTIC ON IMMUNITY IMPROVEMENT IN THE FRY AND SPAWNER PRODUCTION OF PACIFIC WHITE SHRIMP Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Haryanti Haryanti

    2014-12-01

    Full Text Available In order to improve immune system of Pacific white shrimp L. vannamei from any kinds of diseases, various probiotic agents were tested such as Bacillus sp. BC, Alteromonas sp. BY-9 and mixed of both. The probiotics were cultured and added directly to the larval rearing tank (106 cfu/mL equal to 0.5 liter/m3 up to PL-12. In cultured shrimp spawners, probiotic was mixed with feed coated with chitosan, and without adding probiotic as control. In the present experiment, shrimp fry and spawners were reared in tank capacity of 5 m3 and 8 m3. Immunity response was analyzed by quantitative value of the immunity related gene expression with RTqPCR. The results showed that survival rate of shrimp fry cultured with mixed probiotic was 63.16% (PL-8 and 53.46% (PL-12, while control 13.35% (PL-8 and 12.48% (PL-12 respectively. The number of shrimp hemocytes starting from 420 to 530.5 x 104 cells/mL and after challenged with WSSV (for 72 hrs exposure between two treatments of Alteromonas sp. BY-9 and Bacillus cereus BC was not significantly different P>0.05 (402.5 and 432.5 x 104 cells/mL, while in mixed probiotic and control treatments were 391.75 and 229.0 x 104 cells/mL, respectively. The analysis of immunity gene expression revealed that the use of probiotic from larval stages up to spawner has been proved in increasing immunity responses quantified from ProPO activating system (prophenoloxidase/proPO as much as 1.6-2.0 fold, clotting system (transglutaminase, clotting protein of 1.5-2 fold, Antimicrobial Peptide System (anti-LPS factor, crustin, lysozyme, penaiedin2 of 1.5-2.8 fold, and Antioxidant defense mechanism (superoxidase dismutase/SOD, glutathione peroxidase/GPx of 1.8-2.0 fold compared to control.

  15. Management measures to control diseases reported by tilapia (Oreochromis spp.) and whiteleg shrimp (Litopenaeus vannamei) farmers in Guangdong, China

    DEFF Research Database (Denmark)

    Li, Kang; Liu, Liping; Clausen, Jesper Hedegaard

    2016-01-01

    development. Farmers stated lower costs and stricter regulation on antimicrobial usage as reasons for the popularity of probiotics. Farmers also reported the use of herbal extracts for disease control and water quality improvements, partly because of the low number of reported negative side effects...... and pond water quality management. This study investigated the knowledge, practices and challenges of tilapia and whiteleg shrimp farmers when preventing and controlling diseases through the use of antimicrobials and other compounds in Guangdong province, which is the most important shrimp and tilapia...... they did not use antimicrobials to treat shrimp disease. All farmers applied disinfectants and probiotics to control pond water quality although the efficacy of such use was not known. Farmers prepared their own medicated feed through mixing antimicrobial water-based solutions into the feed pellets...

  16. Use of seaweed Ulva lactuca for water bioremediation and as feed additive for white shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Regina Elizondo-González

    2018-03-01

    Full Text Available Two experimental feeding trials were conducted during four weeks to evaluate the use of Ulva lactuca in shrimp culture: (1 for wastewater bioremediation, and (2 using different inclusion levels of U. lactuca meal in shrimp feed. In feeding trial 1, shrimp reared under seaweed U. lactuca water exchange in a re-circulation system (SWE resulted in similar growth and feed utilization as shrimp reared with clean water exchange (CWE. Shrimp under no water exchange (NWE resulted in significant lower growth and higher feed conversion rate (FCR compared to the other treatments (p  0.05. In feeding trial 2, U. lactuca biomass produced by wastewater bioremediation in SWE treatment were dried and ground to formulate diets containing 0, 1, 2, and 3% U. lactuca meal (0UL, 1UL, 2UL, and 3UL. Shrimp fed the 3 UL diet resulted in a significant (p < 0.05 improvement of growth and FCR, and enhanced whole shrimp lipid and carotenoid content by 30 and 60%, respectively, compared to control diet. Seaweed U. lactuca is suggested as a desirable species for wastewater bioremediation in integrated aquaculture systems, and its meal as a good feed additive for farmed shrimp.

  17. Use of seaweed Ulva lactuca for water bioremediation and as feed additive for white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Elizondo-González, Regina; Quiroz-Guzmán, Eduardo; Escobedo-Fregoso, Cristina; Magallón-Servín, Paola; Peña-Rodríguez, Alberto

    2018-01-01

    Two experimental feeding trials were conducted during four weeks to evaluate the use of Ulva lactuca in shrimp culture: (1) for wastewater bioremediation, and (2) using different inclusion levels of U. lactuca meal in shrimp feed. In feeding trial 1, shrimp reared under seaweed U. lactuca water exchange in a re-circulation system (SWE) resulted in similar growth and feed utilization as shrimp reared with clean water exchange (CWE). Shrimp under no water exchange (NWE) resulted in significant lower growth and higher feed conversion rate (FCR) compared to the other treatments ( p   0.05). In feeding trial 2, U. lactuca biomass produced by wastewater bioremediation in SWE treatment were dried and ground to formulate diets containing 0, 1, 2, and 3% U. lactuca meal (0UL, 1UL, 2UL, and 3UL). Shrimp fed the 3 UL diet resulted in a significant ( p  Seaweed U. lactuca is suggested as a desirable species for wastewater bioremediation in integrated aquaculture systems, and its meal as a good feed additive for farmed shrimp.

  18. Determinación del flujo de agua para la biorremediación en sistemas recirculados acuaculturales utilizando tapetes microbianos construidos

    Directory of Open Access Journals (Sweden)

    Ricardo Jiménez-Montealegre

    2015-03-01

    Full Text Available Se evaluó el efecto del flujo de agua sobre la eficiencia de tapetes microbianos para remover nitrógeno amoniacal total (NAT, N-NH4+ + N-NH3, nitritos (N-NO2-, nitratos (N-NO3- y ortofosfatos (P-PO4-3 en un sistema de recirculación piloto para el cultivo de camarón blanco del Pacífico, Litopenaeus vannamei. Se evaluaron cuatro flujos de agua durante un período de 40 días: 5,2 ± 0,3 (A, 6,6 ± 1,3 (B, 8,4 ± 0,3 (C y 12,2 ± 0,7 (D L min-1. Las concentraciones de NAT y nitritos fueron significativamente menores (P 0,05, y su concentración en los sistemas de cultivo alcanzó valores de 4,63 ± 0,76 mg L-1. La tasa de crecimiento de los camarones se mantuvo cercana al valor esperado de 0,7 g semana-1. En este trabajo se demostró que el flujo de agua posee un efecto importante en la capacidad de los tapetes microbianos para la biorremediación de amonio y nitritos en sistemas recirculados. Se recomienda continuar con la identificación de otras variables que podrían afectar el óptimo funcionamiento de los tapetes microbianos.

  19. Explotación de la pesquería de arrastre de camarón durante el período 1991-1999 en el Golfo de Nicoya, Costa Rica

    Directory of Open Access Journals (Sweden)

    Farid A Tabash Blanco

    2007-03-01

    Full Text Available En Costa Rica, la pesquería del camarón que se lleva a cabo en el Golfo de Nicoya desde 1952 representó una de las más importantes actividades económicas de la región. Sin embargo, la sobrepesca redujo los volúmenes de captura al punto de impedir esta actividad comercial. Con el objetivo de evaluar su evolución durante la pasada década y compararla con los registros históricos de captura, se analizaron las estadísticas pesqueras oficiales comprendidas entre 1991 y 1999 para las especies de camarón que se capturan: dos especies de camarón blanco (Litopenaeus occidentalis y L. stylirostris, el camarón tití (Xiphopenaeus riveti, el camarón café (Farfantepenaeus californiensis, el camarón "pinki" (F. brevirostris y el camarón fidel (Solenocera agassizi. Todas las especies analizadas presentaron un alto nivel de sobre-explotación, lo que provoca un aumento en la demanda de reclutas de camarón requeridos para tratar de reponer a los que son capturados por la flota arrastre. En consecuencia, la edad de primera captura ha disminuido exponencialmente. Se recomienda vedar totalmente la pesca de arrastre del camarón en el Golfo de Nicoya.Exploitation of the shrimp trawl fishery in the period 1991-1999 at the Gulf of Nicoya, Costa Rica. In Costa Rica, the Gulf of Nicoya shrimp fishery originated in 1952 and represented one of the most important economic activities in the region. Nevertheless, overfishery reduced the captured volumes to levels that prevent this commercial activity. I analyzed official fishery statistics between 1991 and 1999. These species involved are: two species of white shrimp, (Litopenaeus occidentalis and L. stylirostris, the "tití" shrimp (Xiphopenaeus riveti, the brown shrimp (Farfantepenaeus californiensis, the "pinki" shrimp (F. brevirostris and the "fidel" shrimp (Solenocera agassizi. All the species reached the Maximum Sustainable Yield in the decades of 1970 and 1980 and are now found at over

  20. Neutrons production during the interaction of monoenergetic electrons with a thin tungsten target; Produccion de neutrones durante la interaccion de electrones monoenergeticos con un blanco delgado de tungsteno

    Energy Technology Data Exchange (ETDEWEB)

    Soto B, T. G.; Medina C, D. [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Programa de Doctorado en Ciencias Basicas, 98068 Zacatecas, Zac. (Mexico); Baltazar R, A. [Universidad Autonoma de Zacatecas, Unidad Academica de Ingenieria Electrica, Programa de Doctorado en Ingenieria y Tecnologia Aplicada, 98068 Zacatecas, Zac. (Mexico); Vega C, H. R., E-mail: tzinnia.soto@gmail.com [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Cipres No. 10, Fracc. La Penuela, 98068 Zacatecas, Zac. (Mexico)

    2016-10-15

    When a linear accelerator for radiotherapy operates with acceleration voltages higher than 8 MV, neutrons are produced, as secondary radiation which deposits an undesirable and undesirable dose in the patient. Depending on the type of tumor, its location in the body and the characteristics of the patient, the cancer treatment with a Linac is performed with photon or electron beams, which produce neutrons through reactions (γ, n) and (e, e n) respectively. Because the effective section for the neutrons production by reactions (γ, n) is approximately two orders of magnitude larger than the effective section of the reactions (e, e n), studies on the effects of this secondary radiation have focused on photo neutrons. en a Linac operates with electron beams, the beam coming out of the magnetic deflector is impinged on the dispersion lamella in order to cause quasi-elastic interactions and to expand the spatial distribution of the electrons; the objective of this work is to determine the characteristics of the photons and neutrons that occur when a mono-energetic electron beam of 2 mm in diameter (pencil beam) is made to impinge on a tungsten lamella of 1 cm in diameter and 0.5 mm of thickness. The study was done using Monte Carlo methods with code MCNP6 for electron beams of 8, 10, 12, 15 and 18 MeV. The spectra of photons and neutrons were estimated in 4 point detectors placed at different equidistant points from the center of the lamella. (Author)

  1. Characterization of Rio Blanco retort 1 water following treatment by lime-soda softening and reverse osmosis; Residual brine treated by wet-air oxidation

    Energy Technology Data Exchange (ETDEWEB)

    Kocornik, D.; Renk, R.

    1986-09-01

    Laboratory research has been conducted to evaluate the chemical, physical, and toxicological characteristics of treated and untreated water pumped from the flooded modified in situ retort at lease tract C-a. This wastewater had a total dissolved solids (TDS) content of about 5450 mg/L and a total organic carbon content of about 16 mg/L. Wet chemical analyses, metals analyses, particle-size analyses, and MICROTOX assays were performed on the wastewater before and after treatment by lime-soda softening and reverse osmosis. The reverse osmosis membrane used in this research was a Filmtec model SW30-2521 spiral-wound polyamide unit. In a short duration test at a TDS of 21,800 mg/L, the reverse osmosis system successfully removed dissolved solids and organics from the wastewater. The water was also much less toxic to the MICROTOX organism after treatment by reverse osmosis. Membrane fouling was observed when water with a TDS of 54,500 mg/L was treated. Treatment of the reverse osmosis residual brine was attempted by subcritical wet-air oxidation. The brine remaining after the 170-hour test on the water with a TDS of 5450 mg/L was subjected to temperatures ranging from 204/sup 0/C (400/sup 0/F) to 315/sup 0/C (600/sup 0/F) and pressures from 500 to 1600 psig for approximately 30 minutes. The waste treated by the higher temperatures and pressures showed good removals of organics, nitrogen compounds, and some metals. The sample treated at 302/sup 0/C (575/sup 0/F) and 1300 psi was assayed for MICROTOX response and no toxicity was measured. The reverse osmosis brine was significantly toxic to the MICROTOX organism before treatment by subcritical wet-air oxidation. 14 refs., 8 figs., 14 tabs.

  2. El tejido linfoide asociado a mucosas como blanco del virus de la diarrea vírica bovina: estructura y función

    OpenAIRE

    Romero-Palomo, F.; Ruiz-Villamor, E.; Risalde, M.A.; Molina, V.; Sánchez-Cordón, P.J.; Pedrera, M.; Gómez-Villamandos, J.C.

    2009-01-01

    Las tonsilas, al igual que otros tejidos linfoides asociados a mucosas como las placas de Peyer del intestino delgado, debido a su localización, se presentan como la puerta de entrada de numerosos agentes patógenos, y por sus características estructurales e inmunológicas, protegen los tractos respiratorios y digestivos de los antígenos que son constantemente inhalados e ingeridos. En los bovinos, distintos agentes patógenos replican en las tonsilas en las primeras fases de la infección, desta...

  3. Agrobacterium-mediated genetic transformation of Pogostemon cablin (Blanco) Benth. Using leaf explants: bactericidal effect of leaf extracts and counteracting strategies.

    Science.gov (United States)

    Paul, Anamika; Bakshi, Souvika; Sahoo, Debee Prasad; Kalita, Mohan Chandra; Sahoo, Lingaraj

    2012-04-01

    An optimized protocol for Agrobacterium tumefaciens-mediated transformation of patchouli using leaf disk explants is reported. In vitro antibacterial activity of leaf extracts of the plants revealed Agrobacterium sensitivity to the extracts. Fluorometric assay of bacterial cell viability indicated dose-dependent cytotoxic activity of callus extract against Agrobacterium cells. Addition of 0.1% Tween 20 and 2 g/l L-glutamine to Agrobacterium infection medium counteracted the bactericidal effect and significantly increased the T-DNA delivery to explants. A short preculture of explants for 2 days followed by infection with Agrobacterium in medium containing 150 μM of acetosyringone were found essential for efficient T-DNA delivery. Cocultivation for 3 days at 22 °C in conjunction with other optimized factors resulted in maximum T-DNA delivery. The Agrobacterium-mediated transformation of leaf disk explants were found significantly related to physiological age of the explants, age and origin of the of the donor plant. Leaf explants from second node of the 3-month-old in vivo plants showed highest transformation efficiency (94.3%) revealed by transient GUS expression assay. Plants selected on medium containing 20 mg/l kanamycin showed stable GUS expression in leaves and stem. The elongated shoots readily developed roots on kanamycin-free rooting medium and on transfer to soil, plants were successfully established. Polymerase chain reaction (PCR) and reverse-transcriptase PCR analysis in putative plants confirmed their transgenic nature. The established transformation method should provide new opportunities for the genetic improvement of patchouli for desirable trait.

  4. Measurement of the thickness of a target deposited in a substrate; Medicion del grosor de un blanco depositado en un substrato

    Energy Technology Data Exchange (ETDEWEB)

    Martinez Q, E.; Aguilera, E.F

    1990-12-15

    Being based on the Elastic scattering and in the Energy losses that suffer a projectile to the interacting with the matter, a method that allows to determine the thickness of a target deposited in a more heavy substrate is presented. The obtained results are consistent with that waited and the derived errors of the method are small. The used technique allows to reduce in considerable form the systematic errors coming from the calibration of the equipment. It is considered that this method is applicable in an interval of thickness quite wide and for many materials since it is only necessary to choose the projectile type and the energy of the same one appropriately. (Author)

  5. Comparación productiva de vacas holstein y F1 blanco orejinero (BON x holstein 1. producción y calidad de la leche.

    Directory of Open Access Journals (Sweden)

    Quijano Bernal Jorge H.

    2000-12-01

    Full Text Available Se estimaron los porcentajes de heterosis para la producción de leche, duración de la lactancia y calidad de la leche ( porcentaje de grasa y proteína, en vacas Holstein y F1 (BON x HOLSTEIN del Centro Paysandú, propiedad de la Universidad Nacional de Colombia, situado en el corregimiento Santa Elena, municipio de Medellín, a 2600 m.s.n.m., con temperatura promedio de 12,5C, correspondiendo a la formación bosque muy húmedo montano bajo (bmh-MB. La producción de leche fue afectada significativamente (P 0,01 y proteína (2,8 y 2,9%; P >0,01 para Holstein y F1 BON x Holstein respectivamente. En el BON estos valores fueron 278 kg, 114 días de lactancia, 4,6% de grasa y 3,9% de proteína en la leche. Los porcentajes de heterosis con base en el promedio de razas fueron: 4,20%, 16,70%, -12,80% y -12.20% para producción de leche, duración de lactancia, porcentaje de grasa y porcentaje de proteína respectivamente, todos altamente significativos (P<0,01. Los porcentajes de heterosis tomando como referencia la raza Holstein fueron: -45,20%, -21,50%, 6,25% y 5,35% en el mismo orden de las características anteriormente citadas. En general, los porcentajes de heterosis fueron menores a los reportados en la literatura. Se concluye que el F1 ha desempeñado un papel preponderante en el mejoramiento de la calidad de la leche en el Centro Paysandú, presentado porcentajes de heterosis medios para la calidad de la leche con referencia a la raza Holstein. Esto puede ser confirmado al analizar el porcentaje de grasa desde 1994, cuando parieron las primeras vacas F1, presentado coeficiente de regresión y correlación de 0,02 y 0,55 respectivamente. En la actualidad, el promedio para el porcentaje de grasa y proteína en el hato es de 3,45% y 3,03% respectivamente. Si sólo se tuviese la raza Holstein, estos porcentajes serían de 3,20% y 2,80%, significativamente menores.

  6. Impacto del uso de plaguicidas asociados al cultivo de soja transgénica sobre especies no blanco de la flora riparia y acuática

    OpenAIRE

    Martin, María Laura

    2011-01-01

    La Argentina ha incrementado en la última década ampliamente la superficie de cultivos transgénicos resistentes al glifosato. La gran expansión de este tipo de producción influye en los ambientes acuáticos vecinos a los agroecosistemas. La flora riparia y acuática funciona como sistema amortiguador entre los cultivo y los cursos de agua. Las comunidades desarrolladas en estas franjas se ven afectadas tanto por la restricción de espacio, como por la deriva de plaguicidas sobre las mismas. El o...

  7. Model of two temperatures of the laser evaporation of solid targets; Modelo de dos temperaturas de la evaporacion laser de blancos solidos

    Energy Technology Data Exchange (ETDEWEB)

    Tolentino E, P. [Facultad de Ciencias Fisico Matematicas, BUAP, Puebla (Mexico); Gutierrez T, C.; Camps C, E. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)

    2007-07-01

    The energy transmission in the evaporation process of a solid target by a laser pulse by means of the model of two temperatures which consists on two equations of heat conduction coupled by means of an electron-phonon coupling factor that means the energy transfer rate between the electrons and the net is described. This electron-phonon coupling factor is calculated for the particular case of the graphite, the obtaining of the analytic solutions in a space dimension of the system of non linear partial differential equations is shown considering two forms of the laser pulse (gaussian and delta function) and the electron temperature distributions of temperature and of the net are analyzed. (Author)

  8. Detección de fusarium verticillioides y fumonisinas en granos de maíz blanco provenientes de los estados Yaracuy y Guárico, Venezuela

    OpenAIRE

    Marleny Chavarri; Juan Barroyeta; Yessica Ochoa; Nohants Rumbos; Jesús Alezones

    2017-01-01

    El maíz ( Zea mays L.) es uno de los cereales con mayor superf icie sembrada a nivel mundial, debido a su importancia en la alimentación humana y animal. Son muchos los agentes etiológicos que ocasionan enfermedades en la plantas de maíz; sin embargo, F u sarium verticillioides ( Sacc.) Nirenberg , es uno de los mohos a l os cuales se le ha prestado especial atención por su amplia distribución, especialmente en zonas tropicales y subtropic a les, su ...

  9. Transferencias condicionadas, regímenes de bienestar e igualdad de género: ¿blancos, negros o grises?

    NARCIS (Netherlands)

    K. Voorend (Koen); J.M. Franzoni

    2008-01-01

    textabstractLas transferencias monetarias condicionadas, programas “estrella” de la nueva política latinoamericana, ¿favorecen o atentan contra la igualdad de género? ¿Qué tanto los programas de transferencias condicionadas promueven mayores grados de igualdad como lo argumentan sus defensores? ¿Es

  10. ELABORAÇÃO DE LICOR DE CASCA DE TANGERINA (Citrus reticulata Blanco, VARIEDADE PONKAN, COM DIFERENTES CONCENTRAÇÕES DE CASCA E TEMPOS DE PROCESSAMENTO

    Directory of Open Access Journals (Sweden)

    Elizabeth Luiza de ALMEIDA

    2012-04-01

    Full Text Available No presente trabalho objetivou-se elaborar e avaliar as características físico-químicas e aceitabilidade de licores de casca de tangerina, variedade Ponkan, com graduação alcoólica de 18ºGL e teor de açúcar de 350g.L-1, produzidos com diferentes concentrações de casca e tempos de processamento. Os tratamentos testados foram: 250g de casca e 7 dias de processamento, 250g de casca e 14 dias de processamento, 500g de casca e 7 dias de processamento e 500g de casca e 14 dias de processamento. Nos licores as análises realizadas foram: teor alcoólico, densidade, pH, sólidos solúveis, acidez titulável e teste de aceitabilidade. Na análise estatística o delineamento experimental utilizado foi o inteiramente casualizado (DIC, em esquema fatorial 2 x 2 com o uso do teste de Tukey na comparação das mé- dias dos tratamentos a 1 e 5% de probabilidade. Para o teste de aceitabilidade também foi aplicado o teste de Tukey a 1 e 5% de probabilidade. Nas análises foram utilizadas 6 repetições. Para o teste de aceitabilidade foram utilizados 100 provadores não treinados. O licor correspondente ao tratamento com 500g de casca e 14 dias de processamento, foi o que apresentou melhor comportamento quanto às suas características físico-químicas avaliadas. Todos os licores tiveram boa aceitação sensorial, obtendo médias acima de 7 (gostei regularmente. O licor preparado com a maior concentração de casca e maior tempo de processamento (500g de casca e 14 dias de processamento foi um dos mais aceitos quanto à cor, aroma e textura, embora o mesmo não tenha ocorrido com o quesito sabor.

  11. Evaluación de variedades de maíz blanco en la región Brunca de Costa Rica: experimentación campesina

    OpenAIRE

    Sayra Mungu\\u00EDa; Juan Carlos Sabor\\u00EDo; Hernando C\\u00E1rdenas

    2002-01-01

    En dos comunidades de la región Brunca se evaluaron durante la época de "veranera" (set 1999 - feb 2000), diversos materiales genéticos de maíz incluyendo variedades locales y mejoradas. Esta actividad fue realizada por los Comités de Experimentaci ón de las Asociaciones de Productores de Veracruz de Pejibaye (seis productores) y de Concepción de Pilas (cuatro productores), con el apoyo de la Escuela de Ciencias Agrarias de la Universidad Nacional, el Instituto para el...

  12. Evaluación de variedades de maíz blanco en la región Brunca de Costa Rica: experimentación campesina

    Directory of Open Access Journals (Sweden)

    Sayra Mungu\\u00EDa

    2002-01-01

    Full Text Available En dos comunidades de la región Brunca se evaluaron durante la época de "veranera" (set 1999 - feb 2000, diversos materiales genéticos de maíz incluyendo variedades locales y mejoradas. Esta actividad fue realizada por los Comités de Experimentaci ón de las Asociaciones de Productores de Veracruz de Pejibaye (seis productores y de Concepción de Pilas (cuatro productores, con el apoyo de la Escuela de Ciencias Agrarias de la Universidad Nacional, el Instituto para el Desarrollo y la Acción Social, y la colaboración del Ministerio de Agricultura y Ganadería. En Veracruz, se evaluaron el hí- brido Cargill 343, la variedad mejorada Diamantes 8843 y tres variedades locales denominadas Godo, Mena y Elizondo; las parcelas fueron establecidas por los agricultores basándose en un peso de semilla uniforme (0,5 kg. No hubo diferencias significativas para la producción de grano, entre los materiales evaluados.

  13. Obtention of differential sections in nuclear reactions using a thick target; Obtencion de secciones diferenciales en reacciones nucleares usando un blanco grueso

    Energy Technology Data Exchange (ETDEWEB)

    Chavez R, R

    2000-07-01

    The nuclear reaction analysis (NRA) technique is used mainly for detecting the presence and concentration of light elements of great importance such as: carbon, nitrogen and oxygen. This work has the objective to obtain the differential sections of {sup 16} O and {sup 14} N starting from the irradiation with deuterons of TiSrO{sub 3} samples, of AIN and AIN{sub 2} at energies of 2000 KeV and 1800 KeV respectively. The present work have four chapters; chapter 1 is focused to the physical aspects which takes part in the NRA technique. The technical requirements as well as the necessary equipment for developing the techniques are described in the chapter 2. In chapter 3 it is described the algorithm developed for to obtain the differential sections starting from experimental data, and finally, in chapter 4 are given the results and conclusions. (Author)

  14. Comportamiento productivo del pasto ovillo y ballico perenne solos y asociados con trébol blanco en condiciones de pastoreo.

    OpenAIRE

    Moreno Carrillo, Miguel Angel

    2013-01-01

    Con el objetivo de cuantificar las diferencias en el rendimiento anual y estacional de forraje y la velocidad de crecimiento de dos gramíneas y una leguminosa en función al manejo estacional de la frecuencia de pastoreo, se realizó el presente estudio en el campo experimental del Colegio de Postgraduados, en Montecillo, Texcoco, Estado de México, en una pradera asociada en su primer año de establecimiento. Se evalúo el rendimiento de forraje, tasa de crecimiento, composición botánica y morfol...

  15. Co-Ni/HZSM-5 Catalyst for Hydrocracking of Sunan Candlenut Oil (Reutealis Trisperma (Blanco) Airy Shaw) for Production of Biofuel

    OpenAIRE

    Muttaqii, Muhammad Al; Marlinda, Lenny; Roesyadi, Achmad; Prajitno, Danawati Hari

    2017-01-01

    The production of biofuel by hydrocracking of Sunan candlenut oil as renewable energy can substitute fossil energy. The purpose of this work is to produce biofuel by hydrocracking of Sunan candlenut oil with Co-Ni/HZSM-5 catalyst. The catalyst was prepared by incipient wetness impregnation method. The characterization of catalyst was determined by X-Ray Diffraction (XRD) and nitrogen adsorption-desorption isotherms. The functional groups of the hydrocarbon was determined by Fourier Transform ...

  16. Genotipicación de la resistencia natural del ganado blanco orejinegro “BON” a la Salmonella dublin SL 2260

    Directory of Open Access Journals (Sweden)

    Jorge Eliécer Ossa Londoño

    2000-02-01

    Full Text Available Uno de los factores que controla la resistencia a microorganismos intracelulares como Salmonella y Brucella, es el producto del gen Nramp (proteína del macrófago asociada a resistencia natural; esta proteína, en la fase temprana de la infección, controla la capacidad de replicación de estas bacterias en los macrófagos. Recientemente se identificó asociación entre un alelo de 175 pb de un microsatélite (STR, ligado a Nramp, con la resistencia a microorganismos intracelulares en bovinos; adicionalmente, se han identificado otros tres alelos (177, 179 y 181 pb asociados con susceptibilidad. En la especie bovina, la Salmonella Dublín sirve como modelo para estudiar la resistencia natural a otras bacterias intracelulares como la Brucella abortus, ya que se ha demostrado que macrófagos derivados de bovinos resistentes controlan eficientemente el crecimiento de ambas bacterias. En Colombia, se ha propuesto que el ganado criollo “BON” presenta una marcada resistencia a enfermedades infecciosas, entre ellas la brucellosis. El objetivo de esta investigación fue determinar el genotipo y el fenotipo de la resistencia del ganado BON a la Salmonella Dublín SL 2260, para contribuir a la caracterización inmunogenética de la resistencia natural de este ganado a las infecciones microbianas. En este trabajo sé han analizado 80 bovinos de la raza “BON”, 18 holstein y 4 cebú: se extrajo ADN a partir de sangre periférica, se amplificó el STR ligado al Nramp, a través de la técnica de reacción en cadena de la polimerasa (PCR; el producto fue sometido a un análisis de polimorfismos conformacionales de cadena sencilla (SSCP, utilizando un gel de polyacrilamida al 6% en condiciones no reductoras. De acuerdo a la movilidad electroforética de los amplicones, y comparándola con un patrón ya definido de resistencia, se han tipificado los diferentes animales. De los 80 animales “BON”, 79 (98.75% fueron homocigóticos para el alelo de resistencia (R y solo un animal resulto ser heterocigótico; además, todos los animales de la raza holstein analizados portan el alelo de resistencia. En contraste todos los bovinos cebú presentaron alelos diferentes al alelo de resistencia ya reportado. En muestras que representen las poblaciones R y S se determinará el fenotipo, realizando un ensayo bactericida in vitro, para luego buscar asociación entre genotipos y fenotipos; esta asociación ofrece la posibilidad de seleccionar y hacer cruces con los animales naturalmente resistentes, para contribuir al desarrollo de nuevas estrategias en el control de estos patógenos, y en la caracterización y preservación del ganado criollo colombiano.

  17. Más allá del blanco y negro. Estudio histórico de fotografías coloreadas y su conservación

    Directory of Open Access Journals (Sweden)

    Enara Artetxe Sánchez

    2010-08-01

    Full Text Available La gran mayoría de las colecciones fotográficas antiguas poseen copias coloreadas a mano. El estudio de dichas imágenes y su intervención han sido objeto de estudio en dos proyectos de investigación avalados por el MEC y la UPV/EHU que han sido llevados a cabo por investigadores y profesores de la Universidad del País Vasco. Dichos proyectos han evaluado el efecto de los tratamientos más comunes de conservación-restauración de papel tanto sobre los procedimientos pictóricos seleccionados (acuarelas y óleos como sobre los componentes de la imagen fotográfica en papeles salados, albúminas y copias a la gelatina de revelado químico. Además, este trabajo ha dado como resultado una tesis doctoral presentada en el Departamento de Pintura de la UPV/EHU en el 2009.

  18. Dieta, selectividad y solapamiento trófico entre las tallas del pez blanco Menidia humboldtiana (Atheriniformes: Atherinopsidae en el embalse Tiacaque, México

    Directory of Open Access Journals (Sweden)

    Regina Sánchez

    2013-06-01

    Full Text Available Menidia humboldtiana es una especie nativa muy apreciada por su delicado sabor. Se determinó el espectro trófico, selectividad y solapamiento trófico de ésta, durante 1995 (épocas del año, se obtuvieron muestras de zooplancton e identificaron a nivel genérico. Los peces capturados se agruparon en intervalos de longitud estándar para cada época. Se analizaron los contenidos estomacales (método volumétrico, Laevastu, selectividad (Chesson y solapamiento trófico (Morisita. Se registraron 14 géneros de zooplancton; Bosmina el más abundante (29 625ind/10L seguido por Cyclops (9 496ind/10L ambos en primavera. Los peces pequeños (1-4.9cm consumen a Cyclops en altos porcentajes en primavera e invierno, 61.24-69.82% respectivamente. Ceriodaphnia es consumida por peces de 3-10.9cm y de 13-14.9cm con 72.41-95.5% en verano; en otoño las tallas pequeñas ingieren a Mastigodiaptomus y Ceriodaphnia; Daphnia y Bosmina por peces de 5-8.9cm y los más grandes (9-14.9cm a Ceriodaphnia. M. humboldtiana realiza una depredación selectiva por Ceriodaphnia, Daphnia, Mastigodiaptomus, Bosmina y Cyclops. El solapamiento trófico fue muy marcado entre todas las tallas en primavera, otoño e invierno, a diferencia en verano los peces de 1-2.9 y 11-12.9cm no registraron un solapamiento con otros intervalos de longitud. M. humboldtiana es una especie zooplanctófaga, que realiza una depredación selectiva y un marcado solapamiento trófico entre los intervalos de longitud.

  19. Response of phenolic metabolism to cadmium and phenanthrene and its influence on pollutant translocations in the mangrove plant Aegiceras corniculatum (L.) Blanco (Ac).

    Science.gov (United States)

    Jiang, Shan; Weng, Bosen; Liu, Tao; Su, Yan; Liu, Jingchun; Lu, Haoliang; Yan, Chongling

    2017-07-01

    Polyphenolic compounds are abundant in mangrove plants, playing a pivotal role in the detoxification of pollutants extruded from surrounding environments into plant tissues. The present study aimed to examine the variations of phenolic compounds, namely total polyphenolics, soluble tannins, condensed tannins and lignin, in the mangrove plant Aegiceras corniculatum (L.) due to the presence of exogenous cadmium and phenanthrene and to explore the influence of phenolic metabolism on biological translocation of these pollutants from roots to leaves. After a 6-week exposure to cadmium and phenanthrene, significant accumulations of both pollutants were observed. All determined phenolic compounds in both leaves and roots at high dosage levels were enhanced compared to the uncontaminated plant. Elevations of polyphenols in both treatments are possibly a result of stimulation in the activity of phenylalanine ammonia-lyase (PAL) and the enrichment of soluble sugar. Additionally, a significantly positive dosage relationship between polyphenolic metabolism intensity and phenanthrene contamination levels was found, while the trend observed in cadmium treatment was weak since cadmium at high levels inhibited phenolic production. The enrichment of polyphenols led to a decline in the biological translocation of these pollutants from roots to leaves. The immobilization of pollutants in the plant roots is possibly linked to the adsorption potential of polyphenols. These results will improve the understanding of the tolerance of mangrove plants to exogenous pollutants and will guide the selection of plants in phytoremediation because of the variability of polyphenol concentrations among species. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Determinaci?n de Listeria monocytogenes en quesos blancos artesanales expendidos en la plaza de mercado de C?queza, Cundinamarca

    OpenAIRE

    Baquero Acu?a, Deissy Milena; Bernal Gonz?lez, Astrid Marcela; Campuzano, Silvia

    2006-01-01

    Las intoxicaciones alimentar?as son un problema de salud p?blica, ya que estas se presentan en cualquier tipo de poblaci?n aumentando la prevalencia en personas inmunosuprimidas, mujeres embarazadas y personas de edad avanzada. Seg?n los datos obtenidos, el 80% de las muestras positivas para Listeria spp presentaron Listeria monocytogenes y el 20% Listeria innocua, indicando una prevalencia de la especie pat?gena para los humanos, aumentando el riesgo en la poblaci?n de padecer enfermedades g...

  1. Evolución de la vida útil en panes sin corteza blancos al sustituir el mejorante y sórbico habituales

    OpenAIRE

    Hernando Alonso, David

    2012-01-01

    Los mejorantes son productos utilizados para favorecer el comportamiento de la masa durante el procesado y conseguir mejores propiedades organolépticas del producto final; el ácido sórbico es un conservante que se utiliza para retrasar la aparición de mohos, y se emplea de forma encapsulada para evitar en la medida de lo posible ciertos problemas que este producto presenta con la fermentación de las levaduras y la reología de las masas, afectando por ello al procesado (Legan, 1993). Así, ...

  2. Los grupos escultóricos del Cerrillo Blanco de Porcuna (Jaén. Un ensayo de lectura iconográfica convergente

    Directory of Open Access Journals (Sweden)

    Olmos, Ricardo

    2002-12-01

    Full Text Available Study of the iconographical program of the sculptural complex of Porcuna (Jaén, Spain (2nd half of the 5th. c. B.C., closely related to the history of the aristocratic group who rules this iberian oppidum. The sculptures reflect metaphorically the mythical territory of ancient Obulco and its richness. The orientalizing past is alluded as a part of the aristocratic history. Gender and age groups reflect the oikos´ life. Hunting and wrestling show the education of the youth. The group of warriors evoques a widespread knowledge of epic motifs in the Mediterranean. A common language of nature and power is shared by the Iberian princes.En este texto analizamos el programa iconográfico de Porcuna. Los diversos grupos se articulan en torno a la historia de un linaje aristocrático. La compleja representación de la naturaleza define los diferentes territorios míticos y del oppidum en su relación con la divinidad. Hay una continua alusión temporal al pasado orientalizante, sobre el que se construye la historia del linaje. Grupos de género y de edad articulan el grupo familiar en torno al oikos. La caza y la lucha inician y educan a los jóvenes. Los complejos episodios de la guerra parecen evocar un conocimiento de motivos épicos extendidos en el Mediterráneo. Un lenguaje común puede relacionar a príncipes y aristócratas ibéricos y mediterráneos.

  3. Pre-Harvest Dropped Kinnow ( Citrus reticulata Blanco) Waste Management through the Extraction of Naringin and Pectin from their Peels using Indigenous Resin

    Science.gov (United States)

    Laxmi Deepak Bhatlu, M.; Katiyar, Prashant; Singh, Satya Vir; Verma, Ashok Kumar

    2016-09-01

    About 10-20 % kinnow fruits are dropped in preharvest stage which are waste and are problem to farmer as these create nuisance by rotting and insect rearing ground. The peels of these dropped fruits as well as peels from kinnow processing may be good source of naringin and pectin. Naringin is used in pharmaseutics while pectin is used in food industry. For recovery of naringin and pectn, peels of preharvest dropped kinnow fruits were boiled in water. The extract was passed through macroporus polymeric adsorbent resin Indion PA 800, naringin was adsorbed on it. The adsorbed naringin was desorbed with ethanol. This solution was passed through membrane filter and filtrate was evaporated to obtain naringin. The extract remaining after adsorption of naringin was used to recover pectin using acid extraction method. The recovery of naringin and pectin was about 52 and 58 % respectively. The naringin finally obtained had 91-93 % purity.

  4. Estudio de los tipos de cráteres obtenidos en el impacto de proyectiles 5,56 SS109, sobre blancos de acero AISI-SAE 1045

    Directory of Open Access Journals (Sweden)

    Castro, Álvaro

    2000-02-01

    Full Text Available The present work describes the morphology and formation of the types of craters obtained with the impact of projectiles 5,56 SS109 over seven steel plates AISI-SAE 1045 that received different cycles of heat treatment. A comparison between the type of deformation and fracture structures and the ones generated by the impact of this projectile over one steel armor plate was made. Each one of the craters was characterized by visual and metallography observation. The influence of the plate microstructure on the efficiency for stopping the projectile and the mechanism of craters formation was evaluated.

    En el presente trabajo se describe la morfología y formación de los tipos de cráteres obtenidos, en el impacto de proyectiles 5,56 SS109, sobre siete chapas de acero AISI-SAE 1045 que fueron sometidas a diferentes tratamientos térmicos, comparando el tipo de estructuras de deformación y fractura obtenidas con las formadas por el impacto de uno de estos proyectiles sobre una chapa comercial para blindaje. Cada uno de los cráteres se caracterizó mediante ensayos de inspección visual y metalografía evaluándose, a partir de los resultados, el papel que ejerce la microestructura obtenida sobre el nivel de eficiencia en la detención del proyectil por parte de la chapa y el mecanismo de formación de los cráteres.

  5. Plant growth, Leaf Nutrient status, fruit yield and quality of Nagpur mandarin (Citrus reticulate Blanco) as influenced by potassium (K) fertigation with four potash fertilizer sources

    OpenAIRE

    Parameshwar Sidramappa Shirgure; Anoop Srivastava

    2013-01-01

    To investigate the effect of different potassium (K) fertilizers applied through fertigation system during three different seasons on yield and quality behaviors of 14-16 years Nagpur mandarin an experiment was conducted at National Research Centre for Citrus, Nagpur during 2009-2012. The Nagpur mandarin plants requires potassium which, when applied in different amounts during the flower bud initiation to before fruit maturity can affect the yield and quality of fruit as well. The treatments ...

  6. Human behaviour, benign or malevolent: understanding the human psyche, performing therapy, based on affective mentalization and Matte-Blanco's bi-logic

    OpenAIRE

    Murtagh, Fionn; Iurato, Giuseppe

    2016-01-01

    International audience; The key concept of Ignacio Matte Blanco’s bi-logic is the unavoidable but variable presence of primary process (symmetric thought) in the secondary one (asymmetric thought) ruling consciousness, for every human being. This variable and dynamic presence allows us, by therapeutic intervention, to convert suitably the symmetric thought into the asymmetric one. The former erupts into the latter by means of affectivity which, accordingly, should be suitably treated to be ri...

  7. Dinámica poblacional del rotífero Brachionus ibericus aislado de estanques para camarón, alimentado con diferentes dietas

    Directory of Open Access Journals (Sweden)

    José Cristóbal Román-Reyes

    2014-11-01

    Full Text Available La producción de microalgas vivas para cultivar rotíferos constituye uno de los mayores costos de operación en la larvicultura de peces, por lo que se están desarrollando sustitutos comerciales de microalgas para la alimentación y producción de rotíferos. Se desconoce el efecto que tienen las formulaciones comerciales sobre la dinámica poblacional de rotíferos nativos del noroeste de México y en este estudio se evaluó el efecto de cuatro dietas comerciales (Espirulina, RotiMac®, Nanno 3600®, RotiGrow-Plus® y la microalga viva Nannochloropsis sp., sobre el crecimiento poblacional y fecundidad del rotífero Brachionus ibericus (GenBank KJ949043, aislado de una granja de camarón blanco (Litopenaeus vannamei. Se realizaron cinco réplicas por tratamiento alimenticio utilizando recipientes con volumen de 12 L de agua, con temperatura de 29 ± 1°C y salinidad de 35 ± 1, respectivamente. Los resultados mostraron que durante los periodos experimentales, las dietas probadas resultaron adecuadas para alimentar a B. ibericus, con tasas de crecimiento, tiempos de duplicación, densidades máximas y fecundidades que fluctuaron entre 0.20 a 0.27 rotíferos día-1, 2.60 a 3.42 días, 215 a 344 rotíferos mL-1 y de 0.16 a 0.39 huevos hembra-1, respectivamente. Las diferencias entre tratamientos fueron significativas (P 0.05 a las obtenidas con Nanno 3600®.

  8. Evaluation of jumbo squid (Dosidicus gigas byproduct hydrolysates obtained by acid-enzymatic hydrolysis and by autohydrolysis in practical diets for Pacific white shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Mayra Lizett González-Félix

    2014-09-01

    Full Text Available The marine bioprocessing industry offers great potential to utilize byproducts for fish meal replacement in aquafeeds. Jumbo squid is an important fishery commodity in Mexico, but only the mantle is marketed. Head, fins, guts and tentacles are discarded in spite of being protein-rich byproducts. This study evaluated the use of two jumbo squid byproduct hydrolysates obtained by acid-enzymatic hydrolysis (AEH and by autohydrolysis (AH as ingredients in practical diets for shrimp. The hydrolysates were included at levels of 2.5 and 5.0% of the diet dry weight in four practical diets, including a control diet without hydrolysate. Shrimp growth and survival were not significantly affected by the dietary treatments. Postharvest quality of abdominal muscle was evaluated in terms of proximate composition and sensory evaluation. Significantly higher crude protein was observed in the muscle of shrimp fed the highest hydrolysate levels, AH 5% (204.8 g kg- 1 or AEH 5% (201.3 g kg- 1. Sensory analysis of cooked muscle showed significant differences for all variables evaluated: color, odor, flavor, and firmness. It was concluded that Jumbo squid byproducts can be successfully processed by autohydrolysis or acid-enzymatic hydrolysis, and that up to 5.0% of the hydrolysates can be incorporated into shrimp diets without affecting growth or survival.

  9. Organic carbon source and C/N ratio affect inorganic nitrogen profile in the biofloc-based culture media of Pacific white shrimp (Litopenaeus vannamei)

    OpenAIRE

    Muhammad Hanif Azhar; Eddy Supriyono; Kukuh Nirmala; Julie Ekasari

    2016-01-01

    Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of b...

  10. Organic carbon source and C/N ratio affect inorganic nitrogen profile in the biofloc-based culture media of Pacific white shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Muhammad Hanif Azhar

    2016-03-01

    Full Text Available Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of biofloc media in a conical tank was prepared for each replicate, which consisted of 500 mL of biofloc suspension collected from a shrimp culture unit with biofloc technology application and 4.5 L seawater. Pacific white shrimp culture was performed in 40L glass aquaria at a shrimp density of 30/aquarium. There was a significant interaction between carbon source and the C/N ratio applied (P<0.05. The use of molasses resulted in the highest reduction rate irrespective to the C/N ratio. Keywords: molasses, tapioca, tapioca by product, rice bran, biofloc, total ammonia nitrogen

  11. Influence of oceanographic processes on the early life stages of the blue shrimp ( Litopenaeus stylirostris) in the Upper Gulf of California

    Science.gov (United States)

    Calderon-Aguilera, L. E.; Marinone, S. G.; Aragón-Noriega, E. A.

    2003-02-01

    The possible relationship between circulation patterns and the recruitment of early stages of penaeid shrimp in the Upper Gulf of California was explored by collecting postlarvae (mesh size 0.505 mm) simultaneously in two locations, one off the coast of Sonora (Golfo de Santa Clara: 31°44'49ʺN-114°33'12ʺW) and the other off the Baja California peninsula (San Felipe: 31°11'8.3ʺ N-114°53'13.9ʺW) during two complete fortnightly cycles (July 12-27, 1995 and June 30-July 16, 1996). Individuals with cephalothoracic length from 0.8 to 3.91 mm without a clear size-increasing pattern were found throughout the sampling period, suggesting continuous recruitment to the area. The circulation in the study area was simulated with a three-dimensional baroclinic model forced with tides and climatological hydrography at the mouth of the Gulf of California, and winds and heat and freshwater fluxes at the sea-air interface. Spawning stock surveys have shown that maximum concentration of mature females is near the coast of Sonora (mainland Mexico). The model predicts surface currents of about 8 cm s -1 and suggests that postlarvae found off the coast of the peninsula may come from a different reproductive unit than those found off the mainland coast. This may explain why postlarvae found in Golfo de Santa Clara (mainland) are larger (and, presumably, older) than those found in San Felipe (Baja California). Possible relationships among circulation patterns, lunar cycle, former Colorado River runoffs and time of spawning are discussed.

  12. Utilização da meiofauna bentônica nomonitoramento do cultivo do camarão Litopenaeus vannamei

    OpenAIRE

    Porchat de Assis Murolo, Priscila

    2005-01-01

    Uma das maiores ameaças ao ecossistema estuarino tem sido o rápido aumento na aqüicultura costeira, e em particular a criação de camarões. O efluente da carcinicultura pode ser a principal fonte de poluição nos estuários. Considerando a sensibilidade da meiofauna à entrada de material antrópico e o grupo Copepoda Harpacticoida como o mais sensível à poluentes, este trabalho teve como objetivo, avaliar a influência da carcinicultura no estuário do rio Botafogo (Itapissuma-PE) utilizando a meio...

  13. Dietary ambon lumut banana stem extract Musa cavendishii var. dwarf Paxton as an immunostimulant for white spot disease prevention in Pacific white shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Afriani Ramadhan

    2017-07-01

    Full Text Available ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A; 0.3 (B, and 0.5 (C g/kg, the diet treatment without ambon banana stem extract without challenged test (K-, and diet treatment without ambon banana stem extract with challenged test (K+. Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day. The results showed that the immune responses (average total hemocyte count: 45.15×106 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01, and survival of treatment C (survival rate 100% were higher compared with the treatment K+ (total hemocyte count: 3.83×106 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD,  phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%. Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp. Keywords: Pacific white shrimp, white spot disease, immune response, Ambon banana stem extract  ABSTRAK Penelitian ini bertujuan untuk menguji dosis optimal ekstrak batang pisang ambon melalui pakan dalam meningkatkan respon imun, pertumbuhan dan kelangsungan hidup udang putih terhadap penyakit white spot. Ekstrak batang pisang ambon lumut Musa cavendishii var. dwarf  Paxton menggunakan metode maserasi dengan menggunakan pelarut etanol. Pakan udang yang mengandung ekstrak batang pisang ambon pada dosis yang berbeda selama 29 hari 0,1 (A; 0,3 (B, dan 0,5 (C g/kg, pakan tanpa pemberian ekstrak batang pisang ambon dan tidak diuji tantang (K-, pakan tanpa pemberian ekstrak batang pisang ambon dan diuji tantang (K+. Masing-masing perlakuan terdiri atas tiga ulangan. Frekuensi pemberian pakan empat kali sehari selama 29 hari pemeliharaan. Hasil penelitian menunjukkan bahwa perlakuan C respons imun (rata-rata jumlah total hemosit: 45,15×106 sel/mL, aktivitas phenoloxydase 1,03±0,08 OD, respiratory burst 0,95±0,04 OD, aktivitas fagositik 94,33±1,53%, pertumbuhan (laju pertumbuhan spesifik: 7,79±0,06%/hari, rasio konversi  pakan 0,52±0,01, dan kelangsungan hidup  (survival rate 100% lebih tinggi dibandingkan dengan perlakuan K+ (jumlah total hemosit: 3.83×106 sel/mL, aktivitas phenoloxydase 0,04±0,01 OD, respiratory burst 0,18±0,06 OD, aktivitas fagositik 5,67± 0,58%, laju pertumbuhan spesifik: 2,61±0,08%/hari, rasio konversi pakan 2,11±0,02, nilai kelangsungan hidup: 50%. Jadi, ekstrak batang pisang pada dosis 0,5 g/kg setiap hari pada pakan dapat digunakan untuk meningkatkan pertumbuhan dan sistem kekebalan tubuh nonspesifik terhadap penyakit bintik putih pada udang putih.   Kata kunci: udang putih, white spot, respons imun, ekstrak batang pisang ambon

  14. Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease-producing probiotic, Bacillus subtilis E20, from natto.

    Science.gov (United States)

    Liu, C-H; Chiu, C-S; Ho, P-L; Wang, S-W

    2009-09-01

    To isolate and identify a benefic bacterium, Bacillus subtilis E20, from natto (fermented soybeans), and incorporate it into shrimp feed to promote shrimp growth performance. A protease-producing bacterium, E20, isolated from natto was identified as B. subtilis by an API 50 CHB kit and the 16S rDNA sequence. B. subtilis E20 was able to grow at a broad range of temperatures (10-50 degrees C), pH values (5-10), and NaCl levels (0-9%). The best culture conditions for B. subtilis E20 to produce the protease were 40 degrees C, a pH of 6-8 and 0% NaCl. No shrimp died after being injected with B. subtilis E20 [up to 10(9) colony-forming units (CFU) per shrimp]. Bacillus subtilis E20 was incorporated in diets at the levels of 0 (control), 10(6), 10(7), and 10(8) CFU kg(-1) for shrimp grow-out culture, and results showed that after feeding on B. subtilis E20-containing diets (10(8) CFU kg(-1) of diet), shrimp had excellent growth performance and production compared to the control because protease activities in the digestive tract were improved by B. subtilis E20. Bacillus subtilis E20 isolated from natto is a great protease producer and is able to improve shrimp growth performance through increasing the digestibility of food. Results suggest that B. subtilis E20 is a potential candidate for use as a probiotic to improve shrimp growth performance, and consequently reduce feed costs.

  15. MULTI INFEKSI PADA UDANG Litopenaeus vannamei : DIAGNOSIS DENGAN POLYMERASE CHAIN REACTION (PCR DAN REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR

    Directory of Open Access Journals (Sweden)

    Isti Koesharyani

    2012-04-01

    Full Text Available Penelitian ini dilakukan karena adanya masalah yang dihadapi seperti pertumbuhan udang yang tidak seragam (ukuran bervariasi, penampakan klinis yang abnormal dan organ yang tidak sempurna. Gejala tersebut akibat dari infeksi penyakit yang disebabkan oleh virus. Untuk mengetahui jenis virus yang menyerang udang tersebut, maka dilakukan analisis Polymerase Chain Reaction (PCR dan Reverse TranscriptasePolymerase Chain Reaction (RT-PCR menggunakan berbagai jenis spesifik primer WSSV, IHHNV, MBV, TSV, IMNV, dan PvNV. Sampel udang yang secara visual normal dan abnormal diambil lalu disimpan dalam larutan pengawet 90% Ethanol dan RNAlater kemudian dianalisis di laboratorium dengan metode yang sudah dikembangkan oleh Pusat Penelitian dan Pengembangan Perikanan Budidaya. Hasilnya menunjukkan bahwa udang yang tumbuh lambat dan mempunyai rostrum bengkok dan warna otot daging memutih ternyata tidak hanya diserang oleh satu virus namun dua virus IHHNV dan IMNV. Hasil penelitian ini juga mengindikasikan bahwa udang yang terserang IHHNV akan tumbuh lambat walaupun tidak mematikan, sedangkan udang yang diserang IMNV otot daging di tubuh memutih terutama pada bagian punggung dan dapat menimbulkan kematian.

  16. MULTI INFEKSI PADA UDANG Litopenaeus vannamei : DIAGNOSIS DENGAN POLYMERASE CHAIN REACTION (PCR) DAN REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR)

    OpenAIRE

    Isti Koesharyani; Lila Gardenia; Hambali Supriyadi

    2012-01-01

    Penelitian ini dilakukan karena adanya masalah yang dihadapi seperti pertumbuhan udang yang tidak seragam (ukuran bervariasi), penampakan klinis yang abnormal dan organ yang tidak sempurna. Gejala tersebut akibat dari infeksi penyakit yang disebabkan oleh virus. Untuk mengetahui jenis virus yang menyerang udang tersebut, maka dilakukan analisis Polymerase Chain Reaction (PCR) dan Reverse TranscriptasePolymerase Chain Reaction (RT-PCR) menggunakan berbagai jenis spesifik primer WSSV, IHHNV, MB...

  17. Decreasing of heterogeneity in black tiger prawn (Penaeus monodon and white shrimp (Litopenaeus vannamei broodstocks and their fry produced in hatchery

    Directory of Open Access Journals (Sweden)

    Bambang Widyo Prastowo

    2009-01-01

    Full Text Available Domestication and genetic improvement of shrimp are required towards production of superior broodstock.  BBPBAP Jepara had performed study on evaluation of genetic diversity of black tiger and white shrimp broodstock and their progenies produced in hatchery, using Restriction Fragment Length Polymorphism (RFLP-mtDNA method.  Amplification of black tiger shrimp mtDNA used primers designed based on 16S rDNA sequences, and the products were restricted by Nde II enzyme.  While the primers for white shrimp was based on COIL and COIH sequences, and their PCR products were digested with Nla III.  The results of study showed that heterogeneity of tiger shrimp derived from Aceh waters was 0.1063, while their F1 progeny was 0.0494.  Heterogeneity of male tiger shrimp from Aceh waters and their progeny was 0.2811 and 0.1023, respectively, while of female was 0.1594 and 0.0940, respectively.  Heterogeneity of F1 tiger shrimp from Sunda Strait was 0.0405, while their F2 was homozygous.  Heterogeneity of F1 male and female tiger shrimp from Sunda Strait was 0.0641 and 0.1567, respectively, while of F2 was homozygous for both male and female.  Heterogeneity value of F1 white shrimp from Hawaii was 0.0069, while the F2 was homozygous.  Heterogeneity of F1 male and female white shrimp was 0.0099 and 0.0234, respectively, while of their F2 for both male and female were homozygous.  Homozygous has been found in F1 of white shrimp broodstock from Florida.  Based on the results of study, genetic variation of tiger shrimp and white shrimp broodstock used in hatchery were decrease.  Combination of conventional selective breeding approach and molecular technique may be useful to faster to meet the goal of domestication and quality improvement of penaeid species in Indonesia. Keywords: black tiger shrimp, white shrimp, RFLP, heterogeneity, genetic marker.   ABSTRAK Dalam rangka penyiapan induk-induk udang yang berkualitas unggul, diperlukan usaha untuk domestikasi dan perbaikan mutu genetik udang.  Di BBPBAP Jepara telah dilakukan suatu kajian untuk mengevaluasi keragaman genetik induk udang windu dan udang vaname beserta keturunannya yang dihasilkan dari panti pembenihan dengan metode Restriction Fragment Length Polymorphism (RFLP-mtDNA.  Amplifikasi mtDNA udang windu menggunakan primer yang didisain berdasarkan sekuens 16S rDNA, dan produk PCR dipotong menggunakan enzim Nde II.  Sementara primer untuk udang vaname dibuat berdasarkan sekuens COIL dan COIH, dan produk PCR dipotong dengan enzim Nla III.  Hasil penelitian menunjukkan bahwa nilai heterogenitas populasi induk udang windu asal Aceh dari alam sebesar 0,1063 dan turunan F1-nya sebesar 0,0494. Populasi induk windu jantan Aceh dari alam sebesar 0,2811 dan turunan F1-nya sebesar 0,1023; sedangkan populasi induk windu betina Aceh dari alam sebesar 0,1594 dan turunan F1-nya sebesar 0,0940.  Nilai heterogenitas populasi induk udang windu asal Selat Sunda turunan F1 sebesar 0,0405 dan turunan F2-nya adalah homozigot. Populasi induk windu jantan Selat Sunda turunan F1 sebesar 0,0641 dan turunan F2-nya adalah homozigot; sedangkan populasi induk windu betina Selat Sunda turunan F1 sebesar 0,1567 dan turunan F2-nya adalah homozigot. Nilai heterogenitas populasi induk udang vaname asal Hawaii turunan F1 sebesar 0,0069 dan turunan F2 adalah homozigot.  Populasi induk vaname jantan Hawaii turunan F1 sebesar 0,0099 dan turunan F2-nya adalah homozigot.  Populasi induk betina Hawaii turunan F1 sebesar 0,0234 dan turunan F2-nya adalah homozigot.  Homozigositas pada induk udang vaname asal Florida telah terlihat pada turunan F1-nya. Hasil kajian ini menunjukkan adanya penurunan variasi genetik pada induk udang windu dan udang vaname yang digunakan di panti pembenihan. Kombinasi antara pendekatan selektif breeding konvensional dan aplikasi teknik molekuler mungkin bermanfaat untuk mempercepat tercapainya tujuan domestikasi dan perbaikan kualitas udang penaeid di Indonesia.   Kata kunci : Udang windu, udang vaname, RFLP, heterogenitas, marker genetik

  18. Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

    Science.gov (United States)

    Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; van Wormhoudt, Alain

    2011-03-01

    Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp ( L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5, respectively, and sampled at weeks 1 and 2. Shrimp fed with 40% protein at 25 in salinity (optimal conditions) were used as a control. Regardless of the salinities, shrimp fed with 50% dietary protein had significantly higher growth performance than other diets; no significant differences were found in comparison with the control. Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks. Ambient salinity change also stimulated the hepatosomatic index, which increased in the first week and then recovered to a relatively normal level, as in the control, after 2 weeks. These findings indicate that in white shrimp, the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism. Increased dietary protein level could improve the osmoregulation capacity of L. vannamei with more energy resources allocated to GDH activity and expression.

  19. Molecular characterization of a cDNA encoding vitellogenin in the banana shrimp, Penaeus (Litopenaeus) merguiensis and sites of vitellogenin mRNA expression.

    Science.gov (United States)

    Phiriyangkul, Pharima; Utarabhand, Prapaporn

    2006-04-01

    In order to determine the primary structure of banana shrimp, Penaeus merguiensis, vitellogenin (Vg), we previously purified vitellin (Vt) from the ovaries of vitellogenic females, and chemically analyzed the N-terminal amino acid sequence of its 78 kDa subunit. In this study, a cDNA from this species encoding Vg was cloned based on the N-terminal amino acid sequence of the major 78 kDa subunit of Vt and conserved sequences of Vg/Vt from other crustacean species. The complete nucleotide sequence of Vg cDNA was achieved by RT-PCR and 5' and 3' rapid amplification of cDNA ends (RACE) approaches. The full-length Vg cDNA consisted of 7,961 nucleotides. The open reading frame of this cDNA encoding a precursor peptide was comprised of 2,586 amino acid residues, with a putative processing site, R-X-K/R-R, recognized by subtilisin-like endoproteases. The deduced amino acid sequence was obtained from the Vg cDNA and its amino acid composition showed a high similarity to that of purified Vt. The deduced primary structure, of P. merguiensis Vg was 91.4% identical to the Vg of Penaeus semisulcatus and was also related to the Vg sequences of six other crustacean species with identities that ranged from 86.9% to 36.6%. In addition, the amino acid sequences corresponding to the signal peptide, N-terminal region and C-terminal region of P. merguiensis Vg were almost identical to the same sequences of the seven other reported crustacean species. Results from RT-PCR analysis showed that Vg mRNA expression was present in both the ovary and hepatopancreas of vitellogenic females but was not detected in other tissues including muscle, heart, and intestine of females or in the hepatopancreas of mature males. These results indicate that the Vg gene may be expressed only by mature P. merguiensis females and that both the ovary and hepatopancreas are possible sites for Vg synthesis in this species of shrimp. Copyright 2006 Wiley-Liss, Inc.

  20. Indução do florescimento e crescimento de tangerineira 'Poncã' (Citrus reticulata Blanco em função da irrigação e da aplicação de paclobutrazol Flowering induction and vegetative development of 'Ponkan' mandarin (Citrus reticulata Blanco by irrigation and paclobutrazol application

    Directory of Open Access Journals (Sweden)

    Carlos Henrique dos Santos

    2004-04-01

    Full Text Available O experimento foi conduzido na Faculdade de Ciências Agronômicas - UNESP/Câmpus de Botucatu (SP, com o objetivo de avaliar o florescimento fora de época e o crescimento vegetativo da tangerineira 'Poncã'. Nesse contexto, adotou-se o delineamento estatístico em blocos casualizados, em parcelas subdivididas, com duas repetições, na instalação do ensaio. Os dois tratamentos de -0,03 e -0,05 MPa, como potenciais mínimos da água no solo, constituíram as parcelas e as quatro doses de paclobutrazol: 0; 4; 8 e 12 g por planta, nas subparcelas. No segundo ano de pesquisa, foram mantidas as mesmas parcelas e foram aplicadas nas plantas das subparcelas as doses de 0; 500; 1000 e 2000 mg l-1 de paclobutrazol, via foliar. Cada parcela foi constituída de 16 plantas, sendo oito destinadas à avaliação. Utilizou-se, em ambas as combinações, de um tratamento sem irrigação (-0,07 MPa e sem aplicação de paclobutrazol, como testemunha. Foram avaliados parâmetros, como a altura, diâmetro médio, volume e área de projeção da copa e condutância estomática para caracterizar a resposta das plantas aos tratamentos empregados. Concluiu-se que a aplicação do paclobutrazol e a variação do potencial da água no solo não proporcionaram a indução do florescimento fora de época das tangerineiras, e que os níveis de paclobutrazol influenciaram no desenvolvimento das plantas.The aim of this research was to study the flowering out of season in no induction conditions and the vegetative development of 'Ponkan' mandarin by irrigation and paclobutrazol application. The experiment was carried out at the Faculty of Agricultural Sciences - UNESP/Botucatu, State of São Paulo. The treatments followed a randomized blocks experimental design, being distributed in split-splots, and two replications. Each experimental unit was represented per 16 plants, being 8 destined for the evaluations. The treatments consisted of two soil water potential, -0,03 e -0,05 MPa that constituted the main experimental unit and the paclobutrazol levels that represented the sub units. In the second year of the experiment the units were maintained and it was applied the paclobutrazol: 0; 500; 1000 e 2000 mg L-1, by foliar application, in the plants of the sub units. Both treatments was compared with the treatments without irrigation (control plants: -0,07MPa and whitout paclobutrazol application plants. It was evaluated the following parameters: height, scion diameter medium, scion projection area, scion volume and stomatic condutance showing the plant's response to treatments. It was concluded that the paclobutrazol application and the irrigation did not influence the plant's flowering out of season and that the paclobutrazol levels influenced the growth of 'Ponkan' mandarin plants.

  1. Ahmeti asemel astub lavale Üllar Saaremäe

    Index Scriptorium Estoniae

    2006-01-01

    10. nov. Rakvere Teatris Dajan Ahmetiga peaosas esietenduma pidanud E.-E. Schmitti näidendis "Oscar ja Roosamamma. Kirjad Jumalale" asub Ahmeti asemel Oscarit mängima tüki lavastaja Üllar Saaremäe

  2. "Vargamäe kuningriik". "Oscar ja Roosamamma. Kirjad Jumalale" / Tauno Teder

    Index Scriptorium Estoniae

    Teder, Tauno

    2007-01-01

    Autor soovitab vaadata Endla ja Rakvere Teatri suvelavastust "Vargamäe kuningriik" A. H. Tammsaare "Tõe ja õiguse" I ja V osa ainetel, lavastajaks Jaanus Rohumaa ning E.-E. Schmitti loo järgi sündinud näidendit "Oscar ja Roosamamma. Kirjad Jumalale". Lavastaja ja peaosas Üllar Saaremäe, Rakvere Teater

  3. Teet Kask näitab oma uut, Londonis loodud tantsulavastust / M.T.

    Index Scriptorium Estoniae

    M.T.

    2005-01-01

    28. ja 29. VI toimub Endla teatris rahvusvahelise koostööprojekti esiettekanne Eestis. Etendus "Amor Vincit Omnia" on sündinud lavastaja-koreograafi T. Kase, muusikute Augsti-Knüppeli-Schmitti, hääleseadja Kate Pringle'i ja Londoni linnaülikooli-Labani tudengite koostöös

  4. k.a.s.k. project esitab

    Index Scriptorium Estoniae

    2005-01-01

    28. ja 29. VI toimub Endla teatris rahvusvahelise koostööprojekti esiettekanne Eestis. Etendus "amor vincit omnia" on sündinud lavastaja-koreograafi T. Kase, muusikute Augsti-Knüppeli-Schmitti, hääleseadja Kate Pringle'i ja Londoni linnaülikooli-Labani tudengite koostöös

  5. Lõputa meediakunst Berliinis / Piibe Piirma

    Index Scriptorium Estoniae

    Piirma, Piibe

    2007-01-01

    Meediakunsti festival "Transmediale" alapealkirjaga "Unfinished" Berliini kunstiakadeemias ja linnaruumis 31. I-4. II. Konverentsidest, võidutöödest (Herman Asselberghsi "Proof of Life", Antoine Schmitti "Still Living", Tim Shore'i "Cabinet"), Stelarci etendusest "Fractal Flesh", David Rokeby, Herwig Turki ja Günter Stögeri töödest