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Sample records for black tea polyphenols

  1. Delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract.

    Science.gov (United States)

    Lee, Mao-Jung; Lambert, Joshua D; Prabhu, Saileta; Meng, Xiaofeng; Lu, Hong; Maliakal, Pius; Ho, Chi-Tang; Yang, Chung S

    2004-01-01

    Catechins and theaflavins, polyphenolic compounds derived from tea (Camellia sinensis, fam. Theaceae), have been reported to have a wide range of biological activities including prevention of tooth decay and oral cancer. The present study was undertaken to determine the usefulness of green tea leaves and black tea extract for the delivery of catechins and theaflavins to the oral cavity. After holding either green tea leaves (2 g) or brewed black tea (2 g of black tea leaves in 100 ml) in the mouth for 2-5 min and thoroughly rinsing the mouth, high concentrations of catechins (C(max) = 131.0-2.2 micro M) and theaflavins (C(max) = 1.8-0.6 micro M) were observed in saliva in the 1st hour. Whereas there was significant interindividual variation in the peak levels of catechins and theaflavins, the overall kinetic profile was similar, with t(1/2) = 25-44 min and 49-76 min for catechins and theaflavins, respectively (average coefficient of variation in t(1/2) was 23.4%). In addition to the parent catechin and theaflavin peaks, five unidentified peaks were also observed in saliva after black tea treatment. Hydrolysis of theaflavin gallates, apparently by salivary esterases, was observed in vitro and in vivo. These results indicate that tea leaves can be used as a convenient, slow-release source of catechins and theaflavins and provide information for the possible use of tea in the prevention of oral cancer and dental caries. PMID:14744744

  2. Polyphenol Bioaccessibility and Sugar Reducing Capacity of Black, Green, and White Teas

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    Shelly Coe

    2013-01-01

    Full Text Available Tea (Camellia sinensis is a widely consumed beverage and recognised for its potential enhancing effect on human health due to its rich polyphenol content. While a number of studies have investigated the quantity and type of polyphenols present in different tea samples, no study has reported the potential effect of digestive enzymes on the availability of tea polyphenols for human absorption or the subsequent impact on glycaemic response. The objectives of the present study were to assess the total polyphenol content of different teas, to assess the bioaccessibility of polyphenols in whole and bagged teas, and to determine the effect of black, white, and green tea infusions on sugar release. All of the teas were a significant source of polyphenols (10–116 mg Gallic acid equivalents/g. There was an overall increase in the release of polyphenols from both the bagged and the whole teas following in vitro digestion. Bagged green tea significantly ( reduced rapidly digestible starch from white bread samples compared to control and black and white bagged teas. The present study confirms that tea is a rich source of polyphenols and highlights the potential benefits it may have on modulating glycaemic response in humans.

  3. An essential role of endothelium-derived nitric oxide in vasorelaxations induced by black tea polyphenols

    Institute of Scientific and Technical Information of China (English)

    HUANG Yu

    2008-01-01

    Green tea has received much attention as protective agent against cardiovascular disease and cancer, the two primary targets of preventive medicine. Since our first demonstration in 1999 of the involvement of endothelium-derived nitric oxide in the acute vasodilator effect of green tea polyphenols, several new vascular protective effects of green tea catechins have been identified. Theaflavins are another class of polyphenol pigments found in black tea, however, little is known about their bioactivity in the vascular system. We have recently demonstrated that black tea and its theaflavins cause relaxations of rat aortas via endothelial nitric oxide-dependent mechanisms and the tea polyphenols are very effective in protecting endothelial function agonist oxidative stress. The present results support the vascular benefit of consumption of black tea, which is equal to that of drinking green tea in terms of their endothelial cell protection and antioxidant capacity.

  4. Bioavailability of Tea Polyphenols

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Tea is a pleasant, popular and safe beverage. Tea provides a dietary source of bioactive components to help humans reduce a wide variety of cancer risks and chronic diseases. The antioxidative activity of green tea-derived polypbenols known as catchins has been extensively studied. The reducfive effect is a synergistic action between EGCG,EGC, ECG, EC, pheophytins a and b, and other components in tealeaves, which are more bioavailable for human body.Green tea has a higher content of catechins than black tea. Green tea extract with hot water has high potential and more efficiencytoreducecancerriskthananyotherteaproductsorpureEGCG. Protein, iron, andotherfoodcomponentsmay interfere with the bioavailability of tea polyphenols. Drinkinggreentea (orpolyphenol-rich tea extract ) also enhances the cancer-preventive activity of some cancer-fighting medication such as Sulindac and Tamoxifen. Further studies are required to determine the bioavailability of green tea and cancer-preventive functionality.

  5. Factors affecting the caffeine and polyphenol contents of black and green tea infusions.

    Science.gov (United States)

    Astill, C; Birch, M R; Dacombe, C; Humphrey, P G; Martin, P T

    2001-11-01

    The effects of product and preparation variables on the in-cup chemical composition of tea extracts is of interest because the appearance and taste characteristics and the possible health effects of a tea liquor arise from the chemical components extracted from the leaf during tea preparation. A comprehensive study was therefore undertaken to determine the contributions of product and preparation variables on the total soluble solids, caffeine, and polyphenol contents of tea extracts. The results of this study show that the variety, growing environment, manufacturing conditions, and grade (particle size) of the tea leaves each influence the tea leaf and final infusion compositions. In addition, the composition of the tea infusion was shown to be influenced by whether the tea was contained in a teabag and, if so, the size and material of construction of the bag. Finally, the preparation method, including the amounts of tea and water used, infusion time, and amount of agitation, was shown to be a major determinant of the component concentrations of tea beverages as consumed. An illustration of the variation introduced by these product and preparation factors is provided by comparing solids, caffeine, and polyphenol contents of green and black tea infusions when commercial products are prepared according to the instructions given on their packaging. PMID:11714326

  6. Black tea

    Science.gov (United States)

    ... diuretic to increase urine flow. Some people use black tea for preventing tooth decay and kidney stones. In combination with various other products, black tea is used for weight loss. In foods, ...

  7. Effect of Extraction Conditions on Measured Total Polyphenol Contents and Antioxidant and Antibacterial Activities of Black Tea

    OpenAIRE

    Gokce Polat; Ferda Sari; Y Sedat Velioglu; Nihal Turkmen

    2007-01-01

    Black tea was extracted for 2, 8 and 18 h with absolute acetone, N,N-dimethyl-formamide (DMF), ethanol and methanol and their 50% aqueous solutions. The extracts were screened for total polyphenol contents, antioxidant and antibacterial activities. The polyphenol content of the extracts was found to be in the range of 0.44-114.01 mg gallic acid equivalents (GAE)/g dry weight tea, depending on the solvent used and the length of the extraction process. In general, aqueous acetone or DMF extract...

  8. Black tea

    Science.gov (United States)

    ... product containing black tea extract plus green tea extract, asparagus, guarana, kidney bean, and mate along with a combination of kidney bean pods, garcinia, and chromium yeast for 12 weeks does not reduce body weight ...

  9. Green tea composition, consumption, and polyphenol chemistry.

    Science.gov (United States)

    Graham, H N

    1992-05-01

    Tea is grown in about 30 countries but is consumed worldwide, although at greatly varying levels. It is the most widely consumed beverage aside from water with a per capita worldwide consumption of approximately 0.12 liter per year. Tea is manufactured in three basic forms. Green tea is prepared in such a way as to preclude the oxidation of green leaf polyphenols. During black tea production oxidation is promoted so that most of these substances are oxidized. Oolong tea is a partially oxidized product. Of the approximately 2.5 million metric tons of dried tea manufactured, only 20% is green tea and less than 2% is oolong tea. Green tea is consumed primarily in China, Japan, and a few countries in North Africa and the Middle East. Fresh tea leaf is unusually rich in the flavanol group of polyphenols known as catechins which may constitute up to 30% of the dry leaf weight. Other polyphenols include flavanols and their glycosides, and depsides such as chlorogenic acid, coumarylquinic acid, and one unique to tea, theogallin (3-galloylquinic acid). Caffeine is present at an average level of 3% along with very small amounts of the other common methylxanthines, theobromine and theophylline. The amino acid theanine (5-N-ethylglutamine) is also unique to tea. Tea accumulates aluminum and manganese. In addition to the normal complement of plant cell enzymes, tea leaf contains an active polyphenol oxidase which catalyzes the aerobic oxidation of the catechins when the leaf cell structure is disrupted during black tea manufacture. The various quinones produced by the enzymatic oxidations undergo condensation reactions which result in a series of compounds, including bisflavanols, theaflavins, epitheaflavic acids, and thearubigens, which impart the characteristic taste and color properties of black tea. Most of these compounds readily form complexes with caffeine. There is no tannic acid in tea. Thearubigens constitute the largest mass of the extractable matter in black tea but

  10. Recent advances on tea polyphenols

    OpenAIRE

    Kanwar, Jyoti; Taskeen, Mujtaba; Mohammad, Imthiyaz; HUO, CONGDE; CHAN, TAK HANG; Dou, Qing Ping

    2012-01-01

    Over the past decade many scientific and medical studies have focused on green tea for its long-purported health benefits. There is convincing evidence that tea is a cup of life. It has multiple preventive and therapeutic effects. This review thus focuses on the recent advances of tea polyphenols and their applications in the prevention and treatment of human cancers. Of the various polyphenols in tea, (−)-Epigallocatechin-3-gallate (EGCG) is the most abundant, and active compound studied in ...

  11. Gut Microbial Metabolism of Polyphenols from Black Tea and Red Wine/Grape Juice Is Source-Specific and Colon-Region Dependent

    NARCIS (Netherlands)

    Dorsten, van F.A.; Peters, S.; Gross, G.; Gomez-Roldan, V.; Klinkenberg, M.; Vos, de R.C.; Vaughan, E.E.; Duynhoven, van J.P.M.; Possemiers, S.; Wiele, van der T.; Jacobs, D.M.

    2012-01-01

    The colonic microbial degradation of a polyphenol-rich black tea extract (BTE) and red wine/grape juice extract (RWGE) was compared in a five-stage in vitro gastrointestinal model (TWINSHIME). Microbial metabolism of BTE and RWGE polyphenols in the TWINSHIME was studied subsequently in single- and c

  12. Effect of different brewing times on antioxidant activity and polyphenol content of loosely packed and bagged black teas (Camellia sinensisL.)

    OpenAIRE

    Zeinab Nikniaz; Reza Mahdavi; Seyed Jamal Ghaemmaghami; Neda Lotfi Yaghin; Leila Nikniaz

    2016-01-01

    Objective: Determination and comparison of the effect of infusion time on the antioxidant activity and total polyphenol contents of bagged and loosely packed black teas. Materials and Methods: For twenty loosely packed and eleven bagged tea samples, the antioxidant activity and total polyphenol content were analyzed using FRAP and Folin-Ciocalteau methods, respectively. The ANOVA with Tukey post-hoc test and independent t-test were used for statistical analysis. Results: The antioxidant activ...

  13. Effect of Black Tea and Black Tea Pomace Polyphenols on α-Glucosidase and α-Amylase Inhibition, Relevant to Type 2 Diabetes Prevention

    OpenAIRE

    Striegel, Lisa; Kang, Bouhee; Pilkenton, Sarah J; Rychlik, Michael; Apostolidis, Emmanouil

    2015-01-01

    This study evaluates the potential mechanism of action and bioactivity of black tea and black tea pomace for type 2 diabetes prevention via inhibition of carbohydrate hydrolyzing enzymes. Black tea leaves were extracted in hot water and black tea pomace was extracted in 70% acetone. The phenolic content of the water extract (WBT) and pomace acetone extracts (AOBT) were 5.77 and 8.9 mg/mL, respectively, both based on the same concentration of solid tea in the extract. The water extract was sub...

  14. Tea polyphenols for health promotion

    OpenAIRE

    Khan, Naghma; Mukhtar, Hasan

    2007-01-01

    People have been consuming brewed tea from the leaves of the Camellia sinensis plant for almost 50 centuries. Although health benefits have been attributed to tea, especially green tea consumption since the beginning of its history, scientific investigations of this beverage and its constituents have been underway for less than three decades. Currently, tea, in the form of green or black tea, next to water, is the most widely consumed beverage in the world. In vitro and animal studies provide...

  15. Comparative evaluation of antiproliferative, antiangiogenic and apoptosis inducing potential of black tea polyphenols in the hamster buccal pouch carcinogenesis model

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    Prathiba Duvuru

    2007-01-01

    Full Text Available Abstract Background To evaluate the relative chemopreventive efficacy of two black tea polyphenols, Polyphenon-B [P-B] and BTF-35 on 7,12-dimethylbenz [a]anthracene (DMBA-induced hamster buccal pouch (HBP carcinogenesis. Methods Hamsters were divided into 6 groups. The right buccal pouches of animals in groups 1–3 were painted with 0.5% of DMBA three times a week for 14 weeks. While hamsters in group 1 received no further treatment, animals in groups 2 and 3 received diet containing 0.05% P-B and BTF-35 respectively, four weeks before DMBA painting that was continued until the end of the experiments. Animals in groups 4 and 5 were given P-B and BTF-35 alone respectively as in groups 2 and 3. Group 6 animals served as the untreated control. All the animals were sacrificed after 18 weeks. The expression of p21, cyclin D1, glutathione S-transferase pi (GST-P, nuclear factor kappa B (NF-κB, Bcl-2, Bax, cytochrome C, caspase-3, caspase-9, poly(ADP-ribose polymerase (PARP, cytokeratins and vascular endothelial growth factor (VEGF was analysed by RT-PCR, immunohistochemical and Western blot analyses. Results DMBA treated animals developed buccal pouch carcinomas that displayed increased expression of p21, cyclin D1, GST-P, NF-κB, cytokeratins, VEGF and Bcl-2 with decreased expression of Bax, cytochrome C, caspase-3, caspase-9, and PARP. Dietary administration of both P-B and BTF-35 reduced the incidence of DMBA-induced HBP carcinomas by modulating markers of cell proliferation, cell survival, tumour infiltration, angiogenesis, and apoptosis. Conclusion The results of the present study provide a mechanistic basis for the chemopreventive potential of black tea polyphenols. The greater efficacy of BTF-35 in inhibiting HBP carcinogenesis and modulating multiple molecular targets may have a potential role in the prevention of oral cancer.

  16. Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.

    Science.gov (United States)

    Wu, Tao; Guo, Yu; Liu, Rui; Wang, Kuan; Zhang, Min

    2016-05-18

    With the current changes in diet and living habits, obesity has become a global health problem. Thus, the weight-reducing function of tea has attracted considerable attention. This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats. The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity. A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion. The BT polyphenols and polysaccharides suppressed the formation and accumulation of fat and promoted its decomposition to prevent obesity. PMID:27161951

  17. Black Tea High-Molecular-Weight Polyphenol Stimulates Exercise Training-Induced Improvement of Endurance Capacity in Mouse via the Link between AMPK and GLUT4

    OpenAIRE

    EGUCHI, Tomoaki; Kumagai, Chiaki; Fujihara, Takashi; Takemasa, Thoru; Ozawa, Tetsuo; Numata, Osamu

    2013-01-01

    Aerobic exercise can promote “fast-to-slow transition” in skeletal muscles, i.e. an increase in oxidative fibers, mitochondria, and myoglobin and improvement in glucose and lipid metabolism. Here, we found that mice administered Mitochondria Activation Factor (MAF) combined with exercise training could run longer distances and for a longer time compared with the exercise only group; MAF is a high-molecular-weight polyphenol purified from black tea. Furthermore, MAF intake combined with exerci...

  18. Pharmacokinetics and biotransformation of tea polyphenols.

    Science.gov (United States)

    Qiao, Jinping; Kong, Xiangyi; Kong, Aiying; Han, Mei

    2014-01-01

    Tea is an infusion of the leaves of the Camellia sinensis plant and is the most widely consumed beverage in the world after water. The main chemical components in teas are phenolic compounds (tea polyphenols, mainly tea catechins). A large number of in vitro and in vivo scientific studies have supported that the tea polyphenols can provide a number of health benefits such as, reducing the incidence of coronary heart disease, diabetes and cancer. Recently, tea polyphenols have proven highly attractive as lead compounds for drug discovery programs. A clear understanding of chemistry, stability, pharmacokinetics and metabolic fate of tea will be significant to elucidate many medicinal effects by biochemical theory and pharmaceutical development. This article reviews the current literature on the pharmacoknetics and biotransformation of tea catechins. The half-lives of tea polyphenols are 2-4h and their absorption and elimination are rapid in humans. The peak times (tmax) are 1 and 3 h after oral administration and the peak plasma concentrations are low μM range. It has been reported that catechins are easily metabolized by enzyme and microbe, and the main metabolic pathways are methylation, glucuronidation, sulfation, ring-fission metabolism, and so on. The information is important to discuss some of the challenges and benefits of pursuing this family of compounds for drug discovery. PMID:24527703

  19. The cytogenetic effects of black tea and green tea on cultured human lymphocytes

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    Halil Erhan Eroğlu

    2011-12-01

    Full Text Available In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considerable anticlastogenic and antigenotoxic effects as observed in vitro in human lymphocytes. Thus, it could be concluded that tea polyphenols protected the normal cells from genotoxic or carcinogenic agents, which indicated the therapeutic and antioxidative role of catechins, flavonoids or other tea compounds.

  20. A Review on the Beneficial Effects of Tea Polyphenols on Human Health

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    J. Gupta

    2008-01-01

    Full Text Available The aim of this review is to focus some light on the beneficial effects of the tea polyphenols on human health, based on various laboratory, epidemiological and clinical studies carried out on tea and tea polyphenols in the last few years. Tea is second only to water as the most consumed beverage in the world. Tea has been consumed worldwide since ancient times to maintain and improve health. The health benefits associated with tea consumption have resulted in the wide inclusion of green tea extracts in botanical dietary supplements, which are widely consumed as adjuvants for complementary and alternative medicines. Depending upon the level of fermentation, tea can be categorized into three types: green (unfermented, oolong (partially fermented and black (highly to fully fermented. Black tea represents approximately 78% of total consumed tea in the world, whereas green tea accounts for approximately 20% of tea consumed. Tea is particularly rich in polyphenols, including catechins, theaflavins and thearubigins, which are thought to contribute to the health benefits of tea. Tea polyphenols comprise about one-third of the weight of the dried leaf and they exhibit biochemical and pharmacological activities including antioxidant activities, inhibition of cell proliferation, induction of apoptosis, cell cycle arrest and modulation of carcinogen metabolism. Several studies demonstrate that most tea polyphenols exert their effects by scavenging Reactive Oxygen Species (ROS since excessive production of ROS has been implicated in the development of a variety of ailments including cancer of the prostate gland (CaP. Tea catechins include (--epicatechin (EC,(--epigallocatechin (EGC, (--epicatechin gallate (ECG and (--epigallocatechin gallate (EGCG. These catechins have been shown to be epimerized to (--catechin (C, (--gallocatechin (GC, (--catechin gallate (CG and (--gallocatechin gallate (GCG, respectively, during heat treatment. Tea polyphenols act as

  1. Comparison of black, green and rooibos tea on osteoblast activity.

    Science.gov (United States)

    Nash, L A; Ward, W E

    2016-02-17

    Globally, tea is the second most consumed beverage after water. Habitual tea intake has been associated with higher bone mineral density, particularly in postmenopausal women. This association may be due to its polyphenols and resulting protective antioxidant effects. While in vivo studies have shown improved bone outcomes with a consumption of individual purified tea polyphenols, it is unclear if a particular tea - due to its different profiles of polyphenols - is more beneficial than others. Therefore, we compared three different types of commercially available teas on osteoblasts: green, black and rooibos tea. Tea was normalized to 1 or 10 μg per mL gallic acid equivalents to assess differences in outcomes based on tea profiles rather than the quantity of polyphenol naturally present. The lower level of polyphenols (1 μg per mL gallic acid equivalents) - regardless of tea type and thus polyphenol profile - resulted in greater mineral content as well as cellular and alkaline phosphatase activity in Saos2 cells. Moreover, this was associated with higher markers of differentiation (osteopontin, sclerostin) and reduced cellular toxicity and pro-inflammatory markers (IL6, TNFα). Green, black and rooibos tea improved osteoblast activity at the low level and support epidemiological evidence suggesting tea consumption may benefit bone heath. PMID:26885714

  2. Tea derived galloylated polyphenols cross-link purified gastrointestinal mucins.

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    Pantelis Georgiades

    Full Text Available Polyphenols derived from tea are thought to be important for human health. We show using a combination of particle tracking microrheology and small-angle neutron scattering that polyphenols acts as cross-linkers for purified gastrointestinal mucin, derived from the stomach and the duodenum. Both naturally derived purified polyphenols, and green and black tea extracts are shown to act as cross-linkers. The main active cross-linking component is found to be the galloylated forms of catechins. The viscosity, elasticity and relaxation time of the mucin solutions experience an order of magnitude change in value upon addition of the polyphenol cross-linkers. Similarly small-angle neutron scattering experiments demonstrate a sol-gel transition with the addition of polyphenols, with a large increase in the scattering at low angles, which is attributed to the formation of large scale (>10 nm heterogeneities during gelation. Cross-linking of mucins by polyphenols is thus expected to have an impact on the physicochemical environment of both the stomach and duodenum; polyphenols are expected to modulate the barrier properties of mucus, nutrient absorption through mucus and the viscoelastic microenvironments of intestinal bacteria.

  3. Black tea high-molecular-weight polyphenol stimulates exercise training-induced improvement of endurance capacity in mouse via the link between AMPK and GLUT4.

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    Tomoaki Eguchi

    Full Text Available Aerobic exercise can promote "fast-to-slow transition" in skeletal muscles, i.e. an increase in oxidative fibers, mitochondria, and myoglobin and improvement in glucose and lipid metabolism. Here, we found that mice administered Mitochondria Activation Factor (MAF combined with exercise training could run longer distances and for a longer time compared with the exercise only group; MAF is a high-molecular-weight polyphenol purified from black tea. Furthermore, MAF intake combined with exercise training increased phosphorylation of AMPK and mRNA level of glucose transporter 4 (GLUT4. Thus, our data demonstrate for the first time that MAF activates exercise training-induced intracellular signaling pathways that involve AMPK, and improves endurance capacity.

  4. Antioxidant effects of green tea polyphenols

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The unprecedented interest in the antioxidant activity of green tea polyphenols (GTP) is due to the apparent health benefits of tea drinking and the experimental results with the polyphenols. The results suggest that the galloylated catechins show stronger antioxidant effect than that of nongalloylated catechins and the double bond in C ring also plays an important role in this effect. There are synergic effects between different catechins and the antioxidant effectof (+)-epimers is stronger than that of (-)-epimers. The active site to scavenge free radicals mainly locates in p-hydroxyl of phenol in gallic acid and hydroxyl of phenol in the pyrane also shows activity. The large π bond in chromane and benzene ring of catechin is the structure base for their antioxidant effects. The prevent effect of GTP against diseases and their redox regulation in cell signal pathway are very important to be studied in the future.

  5. Antioxidative and anti-carcinogenic activities of tea polyphenols

    OpenAIRE

    Yang, Chung S; Lambert, Joshua D.; Sang, Shengmin

    2008-01-01

    Tea (Camellia sinensis, Theaceace), a popular beverage consumed world-wide, has been studied for its preventive effects against cancer as well as cardiovascular, neurodegenerative, and other diseases. Most of the proposed beneficial effects have been attributed to the polyphenolic compounds in tea, but the nature of these activities and the molecular mechanisms of their actions remain unclear. Tea polyphenols are known to be strong antioxidants. Prevention of oxidative stress, modulation of c...

  6. Use of tea extracts (Camelia sinensis) in jelly candies as polyphenols sources in human diet.

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    Gramza-Michalowska, Anna; Regula, Julita

    2007-01-01

    Diet rich in polyphenols may be important factor in preventing cardiovascular, neoplastic diseases and slowing down the aging processes. Because tea (Camelia sinensis) is most popular beverage containing relatively large amounts of polyphenols, it could be tremendously important source of polyphenolic constituents in human diet. However, there has been no data on the tea extracts use in particular everyday snacks. Objective of the study was to investigate potential use of tea polyphenol extracts in jelly candies, its taste, colour, consistency and general consumer's acceptance. Sensory analyses were conducted on two kinds of sweet jellies, with gelatin and agar used as thickening agents. As polyphenol source green and black tea extracts (Camellia sinensis) were used at concentration of 1.0% and 1.5%. Total polyphenol content in jellies ranged between 245.9-1256.5 mg/100g of candies and EGCG (epigallocatechin gallate) strong antioxidant content ranged between 3.2-170.1 mg/100g of candies. Sensory analyses included evaluation of overall appearance, colour, taste, aroma, consistence (homogenicity, clot presence) and clarity of jellies. Comparison of two thickening agents resulted in better properties of gelatin jellies according to its quality: colour, clarity, consistence, taste and aroma (ptea extract used it was found that ethanol extracts resulted in lower acceptance for overall acceptance and consistency (ptea polyphenols extracts were accepted by consumers as food product constituents, and might be an interest of wider usage as food components. PMID:17392075

  7. Effect of tea polyphenols on alcoholic liver disease

    Institute of Scientific and Technical Information of China (English)

    Guo-rongHE; Guan-huaDU

    2004-01-01

    AIM: To investigate the scavenging effects of tea polyphenols on aldehyde in vitro and searching for the preliminary mechanism of tea polyphenols (TP) on alcoholic liver disease.METHODS: The effect of aldehyde absorption is tested at gaseous and liquid phases. High performance liquid chromatography (HPLC, HPll00Series) and UV-visible Detector(Wavelength: 235 nm) are used to analyze the components of the outcome of solution reaction. RESULTS: In vitro study showed

  8. Genetic association between the COMT genotype and urinary levels of tea polyphenols and their metabolites among daily green tea drinkers

    OpenAIRE

    Inoue-Choi, Maki; Yuan, Jian-Min; Yang, Chung S.; Van Den Berg, David J.; Lee, Mao-Jung; Gao, Yu-Tang; Yu, Mimi C.

    2010-01-01

    Available in vitro and animal studies have shown cancer protective effects of tea polyphenols. Recent study suggests a greater protective effect of green tea intake on breast cancer risk among women possessing the low-activity associated genotype of the catechol-O-methyltransferase (COMT), which may modulate the metabolism and excretion of tea polyphenols through urine. To determine the effect of COMT genotype on urinary excretion of tea polyphenol metabolites of daily green tea drinkers, a c...

  9. Molecular Mechanisms of Green Tea Polyphenols

    OpenAIRE

    Dou, Q. Ping

    2009-01-01

    Tea, next to water, is the most popular beverage in the world. It has been suggested that tea consumption has the cancer-preventive effects. Epidemiological studies have indicated decreased cancer occurrence in people who regularly drink green tea. Research has also discovered numerous mechanisms of action to explain the biological effects of tea. The most abundant and popular compound studied in tea research is (−)-epigallocatechin-3-gallate or (−)-EGCG, which is a powerful antioxidant and c...

  10. Tea Polyphenols and Their Roles in Cancer Prevention and Chemotherapy

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    Q. Ping Dou

    2008-07-01

    Full Text Available Many plant-derived, dietary polyphenols have been studied for their chemopreventive and chemotherapeutic properties against human cancers, including green tea polyphenols, genistein (found in soy, apigenin (celery, parsley, luteolin (broccoli, quercetin (onions, kaempferol (broccoli, grapefruits, curcumin (turmeric, etc. The more we understand their involved molecular mechanisms and cellular targets, the better we could utilize these “natural gifts” for the prevention and treatment of human cancer. Furthermore, better understanding of their structure-activity relationships will guide synthesis of analog compounds with improved bio-availability, stability, potency and specificity. This review focuses on green tea polyphenols and seeks to summarize several reported biological effects of tea polyphenols in human cancer systems, highlight the molecular targets and pathways identified, and discuss the role of tea polyphenols in the prevention and treatment of human cancer. The review also briefly describes several other dietary polyphenols and their biological effects on cancer prevention and chemotherapy.

  11. The Inhibitory Effect of Chinese Tea and Its Polyphenols on in vitro and in vivo N—Nitrosation

    Institute of Scientific and Technical Information of China (English)

    WUYong-Ning; WANGHuai-Zhou; 等

    1993-01-01

    The objective of this study is to evaluate the possible role of Chinese tea as a natrural inhibitor of N-nitrosation,and to compare the relative inhibitory potency of various kinds of Chinese tea in vitro and in vivo.Studies on the inhibitory effect of 145 samples of Chinese tea on the formation of N-nitrosomorpholine(NMOR)showed that the inhibitory potency of the 7 types of Chinese tea differed greatly,the average blocking rate of green ta(89.04%,n=60),crush,tear,and curl(CTC)black tea(86.99%,n=13),brick tea(85.40%,n=7),fasmine tea (84.99%,n=21),Oolong tea(82.37%,n=9),sun-dried tea(61.99%,n=13)and Chinese Gongfu black tea(54.98%,n=22),wre ositively correlated with thir polyphenols contents.The inhibitory effect of greeen tea and black tea on endogenous N-nitrosation was also confirmed in 3 human volunteer experiments.Drinking tea after meal had a greatr effect than drinking it before meal.It is concluded that Chinese tea can exert a relatively strong inhibitory potency for N-Nitroso compounds(NOC)formation both in vitro and in humans,and the active constitutents may be related to their polyphenols contents,especially the tea catechin derivatives.The results also demonstrated that the amounts of NMOR formed in vitro depended on the molecular structure of tea catechin derivatives and their molar rations to nitrite.

  12. COMPARATIVE STUDY OF GREEN TEA POLYPHENOL AND FLUORIDE ON STREPTOCOCCUS MUTANS

    Institute of Scientific and Technical Information of China (English)

    李鸣宇; 刘正

    2000-01-01

    Objective To disclose the mechanism of green tea polyphenol in the prevention of dental caries.Methods Using sodium fluoride (highly effective anticaries agent) as control and Streptococcus mutans (carigenous microbe) as target, green tea polyphenol (potential bacterial inhibitor) was used to testify its action on the change of microbial morphology, extramicrobial sucrase, glucosyltransferase (GTF), lactate hydrogenase (LDH),protein and pH values of the culture media. Results GTF activities were inhibited both by green tea polyphenol and sodium fluoride, the inhibitory efficacy was stronger in green tea polyphenol. Morphologic changes consisted of membrane disruption in tea polyphenol treated microbes, and cytoplasmic vacuolization in fluoride treated bacteria.High protein level in fluoride treated culture media means cytoplasmic decomposition, intracellular protein leakage into media. Conclusion The inhibition of GTF activity was stronger in green tea polyphenol treated microbes.The target of action was cell membrane disruption in green tea polyphenol treated cell and cytoplasmic in fluoride treated microbes.

  13. The cytogenetic effects of black tea and green tea on cultured human lymphocytes

    OpenAIRE

    Halil Erhan Eroğlu

    2011-01-01

    In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considera...

  14. Chemistry of Secondary Polyphenols Produced during Processing of Tea and Selected Foods

    Directory of Open Access Journals (Sweden)

    Takashi Tanaka

    2009-12-01

    Full Text Available This review will discuss recent progress in the chemistry of secondary polyphenols produced during food processing. The production mechanism of the secondary polyphenols in black tea, whisky, cinnamon, and persimmon fruits will be introduced. In the process of black tea production, tea leaf catechins are enzymatically oxidized to yield a complex mixture of oxidation products, including theaflavins and thearubigins. Despite the importance of the beverage, most of the chemical constituents have not yet been confirmed due to the complexity of the mixture. However, the reaction mechanisms at the initial stages of catechin oxidation are explained by simple quinone–phenol coupling reactions. In vitro model experiments indicated the presence of interesting regio- and stereoselective reactions. Recent results on the reaction mechanisms will be introduced. During the aging of whisky in oak wood barrels, ellagitannins originating from oak wood are oxidized and react with ethanol to give characteristic secondary ellagitannins. The major part of the cinnamon procyanidins is polymerized by copolymerization with cinnamaldehyde. In addition, anthocyanidin structural units are generated in the polymer molecules by oxidation which accounts for the reddish coloration of the cinnamon extract. This reaction is related to the insolubilization of proanthocyanidins in persimmon fruits by condensation with acetaldehyde. In addition to oxidation, the reaction of polyphenols with aldehydes may be important in food processing.

  15. Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes.

    Science.gov (United States)

    Kim, Young Chul; Choi, So Young; Park, Eun Ye

    2015-01-01

    Tea contains polyphenols and is one of the most popular beverages consumed worldwide. Because most tyrosinase inhibitors that regulate melanogenesis are phenol/catechol derivatives, this study investigated the inhibitory effects of Camellia sinensis water extracts (CSWEs), including black tea, green tea, and white tea extracts, on melanogenesis using immortalized melanocytes. CSWEs inhibited melanin accumulation and melanin synthesis along with tyrosinase activity in a concentration-dependent manner. These inhibitory effects were superior to those of arbutin, a well-known depigmenting agent. The anti-melanogenic activity of black (fermented) tea was higher than that of a predominant tea catecholamine, epigallocatechin gallate. CSWEs, especially black tea extract, decreased tyrosinase protein levels in a concentration-dependent manner. These results suggest that the anti-melanogenic effect of CSWEs is mediated by a decrease in both tyrosinase activity and protein expression, and may be augmented by fermentation. Thus, CSWEs could be useful skin-whitening agents in the cosmetic industry. PMID:25643794

  16. Protective Mechanisms of Green Tea Polyphenols in Skin

    Directory of Open Access Journals (Sweden)

    Patricia OyetakinWhite

    2012-01-01

    Full Text Available Skin is frequently exposed to a variety of environmental, chemical, and genotoxic agents that contribute to disease and carcinogenesis. Ultraviolet light (UVR is the main external stress that leads to immunosuppresion, oxidative stress, premature aging, and tumor formation. Scientists and health professionals emphasize the importance of prevention strategies to circumvent such unfavorable outcomes. Plant polyphenols are a promising approach to disease prevention and treatment. Green tea is an abundant source of plant polyphenols that exhibit significant antioxidant, chemopreventive, and immunomodulatory effects in protecting the skin.

  17. The Joint Use of Electronic Nose and Electronic Tongue for the Evaluation of the Sensorial Properties of Green and Black Tea Infusions as Related to Their Chemical Composition

    OpenAIRE

    Susanna Buratti; Antonella Casiraghi; Paola Minghetti; Gabriella Giovanelli

    2013-01-01

    The objectives of the present study were to determine the effects of the brewing method on the amount of the major catechins, methylxanthines, total polyphenols and antioxidant capacity in green and black teas, and to correlate the chemical composition of tea infusions to their sensorial properties by the combined use of the electronic nose and tongue. For this purpose, tea infusions were prepared from 7 green teas and 6 black teas using different extraction times (3 - 5 and 10 min). The extr...

  18. Tea Polyphenols Regulate Key Mediators on Inflammatory Cardiovascular Diseases

    OpenAIRE

    Jun-ichi Suzuki; Mitsuaki Isobe; Ryuichi Morishita; Ryozo Nagai

    2009-01-01

    Tea polyphenols known as catechins are key components with many biological functions, including anti-inflammatory, antioxidative, and anticarcinogenic effects. These effects are induced by the suppression of several inflammatory factors including nuclear factor-kappa B (NF- B). While these characteristics of catechins have been well documented, actions of catechins as mediators on inflammation-related cardiovascular diseases have not yet been well investigated. In this article, we reviewed ...

  19. Protective Mechanisms of Green Tea Polyphenols in Skin

    OpenAIRE

    Patricia OyetakinWhite; Heather Tribout; Elma Baron

    2012-01-01

    Skin is frequently exposed to a variety of environmental, chemical, and genotoxic agents that contribute to disease and carcinogenesis. Ultraviolet light (UVR) is the main external stress that leads to immunosuppresion, oxidative stress, premature aging, and tumor formation. Scientists and health professionals emphasize the importance of prevention strategies to circumvent such unfavorable outcomes. Plant polyphenols are a promising approach to disease prevention and treatment. Green tea is a...

  20. Green tea polyphenols protect spinal cord neurons against hydrogen peroxide-induced oxidative stress

    OpenAIRE

    Zhao, Jianbo; Fang, Shiqiang; Yuan, Yajiang; Guo, Zhanpeng; Zeng, Jinhao; GUO, YUE; Tang, Peifu; Mei, Xifan

    2014-01-01

    Green tea polyphenols are strong antioxidants and can reduce free radical damage. To investigate their neuroprotective potential, we induced oxidative damage in spinal cord neurons using hydrogen peroxide, and applied different concentrations (50–200 μg/mL) of green tea polyphenol to the cell medium for 24 hours. Measurements of superoxide dismutase activity, malondialdehyde content, and expression of apoptosis-related genes and proteins revealed that green tea polyphenol effectively alleviat...

  1. Bioconversion of tea polyphenols to bioactive theabrownins by Aspergillus fumigatus.

    Science.gov (United States)

    Wang, Qiuping; Gong, Jiashun; Chisti, Yusuf; Sirisansaneeyakul, Sarote

    2014-12-01

    Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state Pu-erh tea fermentation. A theabrownins yield of 158 g kg(-1) sun-dried green tea leaves was obtained in 6 days at 45 °C in an aerobic fermentation. In a 2 l fermenter, the yield of theabrownins was 151 g kg(-1) sun-dried green tea leaves in 48 h of aerobic culture (45 °C, 1 vvm aeration rate, 250 rpm agitation speed). Extracellular polyphenol oxidase and peroxidase of A. fumigatus contributed to this bioconversion. Repeated batch fermentation process was used for producing theabrownins but was less productive than the batch process. PMID:25214210

  2. Green tea polyphenols protect spinal cord neurons against hydrogen peroxide-induced oxidative stress

    Institute of Scientific and Technical Information of China (English)

    Jianbo Zhao; Shiqiang Fang; Yajiang Yuan; Zhanpeng Guo; Jinhao Zeng; Yue Guo; Peifu Tang; Xifan Mei

    2014-01-01

    Green tea polyphenols are strong antioxidants and can reduce free radical damage. To investigate their neuroprotective potential, we induced oxidative damage in spinal cord neurons using hy-drogen peroxide, and applied different concentrations (50-200 µg/mL) of green tea polyphenol to the cell medium for 24 hours. Measurements of superoxide dismutase activity, malondial-dehyde content, and expression of apoptosis-related genes and proteins revealed that green tea polyphenol effectively alleviated oxidative stress. Our results indicate that green tea polyphenols play a protective role in spinal cord neurons under oxidative stress.

  3. Direct reduction of N-acetoxy-PhIP by tea polyphenols: a possible mechanism for chemoprevention against PhIP-DNA adduct formation

    International Nuclear Information System (INIS)

    The chemopreventive effect of tea against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-DNA adduct formation and its mechanism were studied. Rats were exposed to freshly prepared aqueous extracts of green tea (3% (w/v)) as the sole source of drinking water for 10 days prior to administration with a single dose of PhIP (10 mg/kg body weight) by oral gavage. PhIP-DNA adducts in the liver, colon, heart, and lung were measured using the 32P-postlabelling technique. Rats pre-treated with tea and given PhIP 20 h before sacrifice had significantly reduced levels of PhIP-DNA adducts as compared with controls given PhIP alone. The possible mechanism of protective effect of tea on PhIP-DNA adduct formation was then examined in vitro. It was found that an aqueous extract of green and black tea, mixtures of green and black tea polyphenols, as well as purified polyphenols could strongly inhibit the DNA binding of N-acetoxy-PhIP, a putative ultimate carcinogen of PhIP formed in vivo via metabolic activation. Among these, epigallocatechin gallate was exceptionally potent. HPLC analyses of these incubation mixtures containing N-acetoxy-PhIP and the tea polyphenols each revealed the production of the parent amine, PhIP, indicating the involvement of a redox mechanism. In view of the presence of relatively high levels of tea polyphenols in rat and human plasma after ingestion of tea, this study suggests that direct reduction of the ultimate carcinogen N-acetoxy-PhIP by tea polyphenols is likely to be involved in the mechanism of chemoprotection of tea against this carcinogen

  4. Isolation and Purification of a Novel Long-chain Acyl Catechin from Lipophilic Tea Polyphenols

    Institute of Scientific and Technical Information of China (English)

    陈平; 杜琪珍

    2003-01-01

    Llpophilic tea polyphenols (LTP) was prepared by esterification of green tea polyphenols (GTP) with hexadecanoyl chloride. A novel long-chaln acyl catechin was isolated and purified from LTP by high-speed countercurrent chromatography (HSCCC).Its molecular structure was elucidated as epigallocatechin-3-O-gallate-4'-O-hexadecanate by elemental analysis, IR, MS and 1H NMR spectra.

  5. Modelling Extraction of White Tea Polyphenols: The Influence of Temperature and Ethanol Concentration

    OpenAIRE

    Sara Peiró; Michael H. Gordon; Mónica Blanco; Francisca Pérez-Llamas; Francisco Segovia; María Pilar Almajano

    2014-01-01

    The optimization of the extraction of natural antioxidants from white tea has fostered intensive research. This study has investigated the effects of ethanol-water mixtures, temperature and time on the extraction of polyphenols and antioxidant components from white tea. The response surface methodology was applied to identify the best extraction conditions. The best conditions to maximize the extraction of total polyphenols were: ethanol, 50%, for 47.5 min. Although the yield of polyphenols w...

  6. Green Tea Polyphenols Extend the Lifespan of Male Drosophila melanogaster While Impairing Reproductive Fitness

    OpenAIRE

    Lopez, Terry; Schriner, Samuel E.; Okoro, Michael; Lu, David; Chiang, Beatrice T.; Huey, Jocelyn; Jafari, Mahtab

    2014-01-01

    Green tea is a popular beverage believed to have many health benefits, including a reduction in the risks of heart disease and cancer. Rich in polyphenolic compounds known as catechins, green tea and its components have been shown to increase the lifespan of various animal models, including Drosophila melanogaster. Here, we investigated the gender-specific effects of green tea on the lifespan of fruit flies and observed that green tea extended the lifespan of male flies only. This effect was ...

  7. Prevention of coronary heart disease and cancer by tea, a review

    OpenAIRE

    Weisburger, John H

    2003-01-01

    Biomedical research has uncovered the mechanisms whereby tea promotes good health and lowers the risk of major chronic diseases, such as heart disease and many types of cancer. The active components in tea are polyphenols, epigallocatechin gallate in green tea, theaflavins and thearubigins in black tea. Green and black tea and the polyphenols have similar beneficial effects. The mechanisms are categorized into 5 groups. 1) Tea polyphenols are powerful antioxidants. They decrease the oxidation...

  8. Physiological activity of irradiated green tea polyphenol on the human skin.

    Science.gov (United States)

    An, Bong-Jeun; Kwak, Jae-Hoon; Son, Jun-Ho; Park, Jung-Mi; Lee, Jin-Young; Park, Tae Soon; Kim, So-Yeun; Kim, Yeoung-Sun; Jo, Cheorun; Byun, Myung-Woo

    2005-01-01

    Physiological activity of irradiated green tea polyphenol on the human skin was investigated for further industrial application. The green tea polyphenol was separated and irradiated at 40 kGy by y-ray. For an anti-wrinkle effect, the collagenase inhibition effect was higher in the irradiated sample (65.3%) than that of the non-irradiated control (56.8%) at 200 ppm of the concentration (p green tea polyphenol did not change and even increased its anti-wrinkle, skin-whitening and anticancer effects on the human skin. The results indicated that irradiated green tea polyphenol can be used as a natural ingredient with excellent physiological functions for the human skin through cosmetic or food composition. PMID:16173528

  9. Chemo prevention of Tea Polyphenols against Tumor Growth of Hepato-Colon Cancer Induced by Azoxy methane in Rats

    International Nuclear Information System (INIS)

    This investigation was conducted to evaluate the chemo prevention of tea polyphenols as anticancer agent in rats which were injected with azoxy methane (AOM) which is a potent hepato-colon carcinogen agents in rodents. The obtained data revealed a significant elevation in serum tumor markers, carcino-embryonic antigen (CEA), alpha-fetoprotein (AFP) and cancer antigen (CA19.9) in carcinogenic rats in comparison to their corresponding normal control ones. Also, there was a significant increase in the content of cytochrome P450 and the activity of alcohol dehydrogenase (ADH) in both liver and colon as well as a significant elevation in the activities of methoxyresorufin-O-dealkylase (MRD), ethoxyresorutin-O-dealkylase (ERD) and pentoxyresorufin-O- dealkylase (PRD) in liver microsomes. While, glutathione content (GSH) and glutathione peroxidase (Gpx) activity were decreased significantly in liver and colon as a result of cancer induction. On the other hand, the supplementation of black or green tea before induction of cancer in rats led to a considerable correction in all previous parameters studied. These amelioration effects dependent on magic biochemical properties of flavanols (catechins) and type of tea. In conclusion, tea polyphenols have appreciable anti-cancer efficacy on hepato colon cancer in rats. The underlying mechanisms of through which tea counteracted hepato-colon cancer were discussed

  10. Combined Effects of Green Tea Extracts, Green Tea Polyphenols or Epigallocatechin Gallate with Acarbose on Inhibition against α-Amylase and α-Glucosidase in Vitro

    OpenAIRE

    Yiqi Wang; Danielle Hochstetter; Ping Xu; Yuefei Wang; Junjie Gao

    2013-01-01

    Green tea, green tea polyphenols and epigallocatechin gallate (EGCG) are confirmed to have beneficial effects in the treatment of diabetes mellitus, and a possible mechanism can be ascribed to their inhibitory effect against α-amylase and α-glucosidase in the digestive tract. In this paper, we first investigated the combined inhibitory effect of green tea extracts, green tea polyphenols or EGCG with acarbose on α-amylase and α-glucosidase in vitro. Our results indicated that the interaction b...

  11. Antioxidant Effects of Green Tea and Its Polyphenols on Bladder Cells

    OpenAIRE

    Coyle, Christian H; Philips, Brian J.; Morrisroe, Shelby N.; Chancellor, Michael B.; Yoshimura, Naoki

    2008-01-01

    Genitourinary tract inflammation/ailments affect the quality of life and health of a large segment of society. In recent years, studies have demonstrated strong anti-oxidant effects of green tea and its associated polyphenols in inflammatory states. This in vitro study examined the antioxidant capabilities (and putative mechanisms of action) of green tea extract (GTE), polyphenon-60 (PP-60, 60 % pure polyphenols), (−)-epicatechin-3-gallate (ECG) and (−)-epigallocatechin-3-gallate (EGCG) in no...

  12. New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate

    OpenAIRE

    Hae-Suk Kim; Quon, Michael J.; Jeong-a Kim

    2014-01-01

    Green tea is rich in polyphenol flavonoids including catechins. Epigallocatechin 3-gallate (EGCG) is the most abundant and potent green tea catechin. EGCG has been extensively studied for its beneficial health effects as a nutriceutical agent. Based upon its chemical structure, EGCG is often classified as an antioxidant. However, treatment of cells with EGCG results in production of hydrogen peroxide and hydroxyl radicals in the presence of Fe (III). Thus, EGCG functions as a pro-oxidant in s...

  13. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells

    International Nuclear Information System (INIS)

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. - Highlights: • Tea polyphenol

  14. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Jin [Key Laboratory of Tea Biochemistry and Biotechnology of Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036 (China); College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Li, Feng [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Fang, Yong; Yang, Wenjian [College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China); An, Xinxin; Zhao, Liyan; Xin, Zhihong; Cao, Lin [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Hu, Qiuhui, E-mail: qiuhuihu@njau.edu.cn [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China)

    2014-03-01

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. - Highlights: • Tea polyphenol

  15. Inhibition of free radical induced oxidative hemolysis of red blood cells by green tea polyphenols

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological membranes and the inhibitory effect of natural antioxidants. The hemolysis was induced by a water-soluble free radical initiator 2,2′-azo(2- asmidinopropane)dihydrochloride (AAPH) and inhibited by the principal polyphenolic components extracted from green tea leaves, i.e. (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicat- echin gallate (ECG), (-)-epigallocatechin gallate (EGCG) and gallic acid (GA). Addition of AAPH at 37°C caused fast hemolysis after a short period of inhibition period, while addition of the green tea polyphenols efficiently suppressed the hemolysis in the activity sequence of EGCG>EGC>ECG≈EC>GA, demonstrating that these green tea polyphenols are effective antioxidants which could protect biological membranes from free radical induced oxidative damage.

  16. Production, Composition, and Health Effects of Oolong Tea

    OpenAIRE

    İlkay Koca; Şeyda Bostancı

    2014-01-01

    Tea, one of the most widely consumed beverages in the world, is produced from the leaves of the plant Camellia sinensis L.. Tea has important physiological properties and potential health benefits due to the presence of compounds such as polyphenols, amino acids, vitamins, carbohydrates, caffeine, and purine alkaloids. Tea is produced in three types as green tea (unfermented), oolong tea (partially fermented), and black tea (fully fermented). Black tea is consumed worldwide, whereas green and...

  17. Cancer Preventive Mechanismsof the Green Tea Polyphenol (-)-Epigallocatechin-3-gallate

    OpenAIRE

    Hong-Yu Zhang; Lei Chen

    2007-01-01

    Accumulating evidence indicates that consumption of tea, especially green tea, is good for preventing cancer. To elucidate the cancer preventive mechanisms of green tea, much effort has been devoted to investigating the anticancer effects of (-)-epigallocatechin-3-gallate (EGCG), the major component of green tea. It has been revealed that EGCG restrained carcinogenesis in a variety of tissues through inhibition of mitogen-activated protein kinases (MAPK), growth factor-related cell signaling,...

  18. Purification process influence on green tea extracts’ polyphenol content and antioxidant activity

    OpenAIRE

    Anna Gramza-Michałowska; Józef Korczak; Marzanna Hęś

    2007-01-01

    The research examined green tea ethanol extract, subjected to different purification processes with use of active carbon, bleaching earth, and mixture of acetone, acetic acid, water, with or without earlier hexane treatment. Purified extracts were examined according to total polyphenols content, antioxidant activity in linoleic acid emulsion and antiradical activity in DPPH• radical scavenging method. Highest polyphenol content was evaluated in the extract purified with bleaching ea...

  19. Validation of Green Tea Polyphenol Biomarkers in a Phase II Human Intervention Trial

    OpenAIRE

    Wang, Jia-Sheng; Luo, Haitao; Wang, Piwen; Tang, Lili; YU, JIAHUA; Huang, Tianren; Cox, Stephen; Gao, Weimin

    2007-01-01

    Health benefits of green tea polyphenols (GTPs) have been reported in many animal models, but human studies are inconclusive. This is partly due to a lack of biomarkers representing green tea consumption. In this study, GTP components and metabolites were analyzed in plasma and urine samples collected from a phase II intervention trial carried out in 124 healthy adults who received 500- or 1,000-mg GTPs or placebo for 3 months. A significant dose-dependent elevation was found for (-)-epicatec...

  20. Green Tea Polyphenols Function as Prooxidants To Activate Oxidative-Stress-Responsive Transcription Factors in Yeasts▿

    OpenAIRE

    Maeta, Kazuhiro; Nomura, Wataru; Takatsume, Yoshifumi; Izawa, Shingo; Inoue, Yoshiharu

    2006-01-01

    Epigallocatechin gallate (EGCG) is the most abundant polyphenolic flavonoid in green tea. Catechin and its derivatives, including EGCG, are widely believed to function as antioxidants. Here we demonstrate that both EGCG and green tea extract (GTE) cause oxidative stress-related responses in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe under weak alkaline conditions in terms of the activation of oxidative-stress-responsive transcription factors. GT...

  1. Green Tea Polyphenols for the Protection against Renal Damage Caused by Oxidative Stress

    OpenAIRE

    Takako Yokozawa; Jeong Sook Noh; Chan Hum Park

    2012-01-01

    Green tea, prepared from the leaves of Camellia sinensis L., is a beverage that is popular worldwide. Polyphenols in green tea have been receiving much attention as potential compounds for the maintenance of human health due to their varied biological activity and low toxicity. In particular, the contribution of antioxidant activity to the prevention of diseases caused by oxidative stress has been focused upon. Therefore, in this study, we investigated the effects of (−)-epigallocatechin 3-O-...

  2. Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons

    OpenAIRE

    Lin Cong; Chang Cao; Yong Cheng; Xiao-Yan Qin

    2015-01-01

    Green tea polyphenols are a natural product which has antioxidative and antiapoptotic effects. It has been shown that glutamate excitotoxicity induced oxidative stress is linked to neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In this study we explored the neuroprotective effect of green teen polyphenols against glutamate excitotoxicity in the primary cultured cortical neurons. We found that green tea polyphenols protected against glutamate induced neurotoxic...

  3. Total polyphenols content and antioxidant activity of different tea commercially produced in Nepal

    OpenAIRE

    Shrestha, Rojeena; Lama, Jiwan Prava; Shrestha, Kshitij

    2010-01-01

    This study was conducted to assess and compare the total polyphenol content (TPC), antioxidant activity of different tea varieties commercially produced in Nepal. Nine different brands of tea samples, three from each CTC, orthodox and green tea were collected from different parts of Kathmandu valley. TPC was analyzed as per folin-ciocalteu method and antioxidant activity as per DPPH (2, 2-diphenyl-1-picrylhrdrazine) free radical scavenging capacity. The TPC was found to be 4.60, 8.79, 18.20mg...

  4. Excretion of Four Catechins in Tea Polyphenols in Rats

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To investigate excretion profiles of the four major anti-oxidant active catechins, (-) epigallo-catechin-3-gallate (EGCG), (-) epicatechin-3-gallate (ECG), (-) epigallocatechin (EGC), and epicatechin (EC) in tea polyphenols (TP) in rats in order to provide experimental data for clinical uses and development of TP as a novel drug. Methods The above four catechins in urine, bile, and feces were simultaneously determined by high performance liquid chromatography coupled with ultraviolet absorption detector (HPLC-UV) assay with a binary gradient elution. The samples were extracted by ethyl acetate prior to HPLC. The quantification was carried out by peak area internal standard method. Following iv dosing TP 100 mg/kg to rats, the samples were collected at different time intervals up to 8 h (urine and bile) and 24 h (feces). Results The urinary Ae, 0-8 h (cumulative excretion amount over 8 h) of EGCG, ECG, EGC, and EC were, on the average, 150.83, 30.75, 116.69, and 254.56 μg, corresponding to fe, 0-8 h (cumulative excretion fraction of dose over 8 h) of 1.45%, 0.84%, 7.88%, and 10.73%, respectively; the biliary Ae, 0-8 h were 12.61, 42.64, 6.61, and 1.24 μg, corresponding to the fe, 0-8 h of 0.12%, 1.16%, 0.45%, and 0.053%,respectively. For fecal excretion, only EGCG and EGC were detected with Ae, 0-24 h of 7.38 μg (fe, 0-24 h of 0.07%) and 157 μg (fe, 0-24 h of 9.99 %), respectively. The fe, total (the total fe of 3 excretory routes) were 18.32%, 10.78%, 2.00%, and 1.64% for EGC, EC, ECG, and EGCG, respectively. Conclusion EGCG and EC are mainly excreted in urine, ECG in bile, and EGC in feces by reference to their Ae and fe. The excretion of the four catechins based on fe, total is ranked in order of EGC > EC > ECG > EGCG. Only small amount of four catechins are recovered in urine, bile, and feces, indicating an extensive metabolic conversion of catechins in the rat body.

  5. HPLC-DAD-MS/MS analysis of purine alkaloids and tea polyphenols in young leaves of Yinghong 1, Yinghong 9 and Qimen cultivars

    OpenAIRE

    J. Chen; Lu, J; Wang, D; Miao, A; D. Yang

    2009-01-01

    Using the HPLC-DAD-MS/MS method, two purine alkaloids, seven catechins, and two non-catechin type of polyphenols were identified in young leaves of Yinghong 1, Yinghong 9 and Qimen cultivars mainly made for black tea. According to the quantitative results, the contents of catechins in the leaves of Yinghong 1 and Qimen are similar, while Yinghong 9 is quite different, with ECG higher than EGCG.

  6. Green tea polyphenols attenuate glial swelling and mitochondrial dysfunction following oxygen-glucose deprivation in cultures

    Science.gov (United States)

    Astrocyte swelling is a major component of cytotoxic brain edema in ischemia. Oxidative stress and mitochondrial dysfunction have been hypothesized to contribute to such swelling in cultures. We investigated the protective effects of polyphenol-rich green tea extract (GTE) on key features of ischemi...

  7. Green tea polyphenols supplementation improves bone microstructure in orchidectomized middle-Aged rats

    Science.gov (United States)

    Our recent study shows that green tea polyphenols (GTP) attenuate trabecular bone loss in ovariectomized middle-aged female rats. To investigate whether GTP prevents bone loss in male rats, 40 rats with and without oriectomy (ORX) were assigned to 4 groups in a 2 (sham vs. ORX)× 2 (no GTP and 0.5% G...

  8. Antiradiation effect of tea polyphenol and effective components from ginkgo leaves

    International Nuclear Information System (INIS)

    In order to explore the antiradiation effect of tea polyphenol (TP) and ginkgo biloba leaves extracts, authors have made an animal experiment, 6 groups of Kunming rats, 12 rats in each group, were investigated. Except contrast group, other 5 groups were fed with different medicament for 20 d, then all 6 groups were irradiated by 60Co γ-rays of 9 Gy/min for one minute. After the irradiation the rats were continually fed with the same medicament for 13d. Meanwhile we observed and recorded the death number of each group day by day. Compared with contrast, the results showed that increased living ratios of tea polyphenol, flavonoids, ginkgolide, 'TP plus ginkgolide' and 'TP plus flavonoids' groups were 66.6%, 41.6%, 75%, 66.6% and 75%, and their increased average living time were 2.9 d (P<0.01), 1.8 d (P<0.05), 3.2 d(P<0.01), 3.1 d(P<0.01) and 3.1 d (P<0.01), respectively. The experimental results indicate that tea polyphenol and ginkgo biloba extracts have an antiradiation ability, for the poly-hydroxyl of tea polyphenol, the hydroxyl and ester bond of ginkgolide are suspected to be the foundation of the ability. It is found that the coordination effect of two materials is stronger than any one alone as they have the same chemical structures or similar functional groups. (authors)

  9. In Vitro Biocompatibility of Nanoscale Zerovalent Iron Particles (NZVI) Synthesized using tea-polyphenols.

    Science.gov (United States)

    A “green” protocol was used for the rapid generation of nanoscale zerovalent iron (NZVI) particles using tea polyphenols. The NZVI particles were subsequently examined for in vitro biocompatibility using the human keratinocyte cell (HaCaT) line as a skin exposure model. The cell...

  10. Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments

    International Nuclear Information System (INIS)

    Tea polyphenols have been reported to be potent antioxidants and beneficial in oxidative stress related diseases. Prooxidant effects of tea polyphenols have also been reported in cell culture systems. In the present study, we have studied oxidative stress in the subcellular compartments of PC12 cells after treatment with different concentrations of the green tea polyphenol, epigallocatechin-3-gallate (EGCG). We have demonstrated that EGCG has differentially affected the production of reactive oxygen species (ROS), glutathione (GSH) metabolism and cytochrome P450 2E1 activity in the different subcellular compartments in PC12 cells. Our results have shown that although the cell survival was not inhibited by EGCG, there was, however, an increased DNA breakdown and activation of apoptotic markers, caspase 3 and poly- (ADP-ribose) polymerase (PARP) at higher concentrations of EGCG treatment. Our results suggest that the differential effects of EGCG might be related to the alterations in oxidative stress, GSH pools and CYP2E1 activity in different cellular compartments. These results may have implications in determining the chemopreventive therapeutic use of tea polyphenols in vivo

  11. Fungal isolates from a Pu-erh type tea fermentation and their ability to convert tea polyphenols to theabrownins.

    Science.gov (United States)

    Wang, Qiuping; Gong, Jiashun; Chisti, Yusuf; Sirisansaneeyakul, Sarote

    2015-04-01

    The natural microbiota involved in the fermentation influence the quality and taste of fully postfermented teas such as China's Pu-erh tea. Ten microbial isolates representing 6 species were recovered from a solid-state fermentation of a Pu-erh type tea. The isolates were Aspergillus tubingensis, Aspergillus marvanovae, Rhizomucor pusillus, Rhizomucor tauricus, Aspergillus fumigatus, and Candida mogii. With the exception of A. marvanovae and C. mogii, all these microorganisms have been previously reported in solid-state fermentations of native Pu-erh tea. The ability of the isolates for converting the tea polyphenols to bioactive theabrownins in infusions of sun-dried green tea leaves in a submerged fermentation process was subsequently investigated. All isolates except C. mogii TISTR 5938 effectively produced theabrownins in a 4-d fermentation in shake flasks at 40 °C, 250 rpm. A. tubingensis TISTR 3646, A. tubingensis TISTR 3647, A. marvanovae TISTR 3648, and A. fumigatus TISTR 3654 produced theabrownins at particularly high levels of 6.5, 12.4, 11.1, and 8.4 g/L, respectively. PMID:25799937

  12. A method for producing superfine black tea powder with enhanced infusion and dispersion property.

    Science.gov (United States)

    Xiao, Weihua; Zhang, Yang; Fan, Chongxin; Han, Lujia

    2017-01-01

    Tea is rich in healthy components including polyphenols, caffeine, gallic acids, and others. Current technology of tea infusion and extraction leads to more than 40% soluble solids wasted in spent leaf. To increase the bioaccessibility of black tea, we report a method of pulverization treatments including general and superfine grinding to reduce the particle size. In comparison with coarsely ground black tea powders (BTPs), the superfine ground BTP with medium diameter 6.9μm resulted in significant higher infusion yield of total polyphenols (TPP), caffeine, and water-soluble carbohydrate (WSC). The total water-soluble solids (WSS) of superfine BTP infusion increased markedly by twice due to the accelerated diffusion and enhanced solubility. High correlation between particle size and sedimentation ratio suggested improved dispersion stability of superfine BTP. The optimal dispersion of 0.1% superfine BTP in water was obtained by combination of homogenization and 0.08% CMC-Na formulation with 27.05% centrifugal sedimentation ratio. PMID:27507472

  13. Purification process influence on green tea extracts’ polyphenol content and antioxidant activity

    Directory of Open Access Journals (Sweden)

    Anna Gramza-Michałowska

    2007-06-01

    Full Text Available The research examined green tea ethanol extract, subjected to different purification processes with use of active carbon, bleaching earth, and mixture of acetone, acetic acid, water, with or without earlier hexane treatment. Purified extracts were examined according to total polyphenols content, antioxidant activity in linoleic acid emulsion and antiradical activity in DPPH• radical scavenging method. Highest polyphenol content was evaluated in the extract purified with bleaching earth, lowest however in purified with solvents mixture. Antioxidant activity of green tea extracts in linoleic acid emulsion indicated that highest antioxidative effectivity coefficient (Aec values represented sample purified with active carbon and solvents mixture. Highest DPPH• radical scavenging activity was found in sample purified with solvents mixture, other samples however represented close activity. The present research indicated that plant extracts purification processes resulted in decrease of total polyphenols content, however without its antioxidant activity decrease.

  14. Consumption of coffee, but not black tea, is associated with decreased risk of premenopausal breast cancer.

    Science.gov (United States)

    Baker, Julie A; Beehler, Gregory P; Sawant, Abhishek C; Jayaprakash, Vijayvel; McCann, Susan E; Moysich, Kirsten B

    2006-01-01

    Caffeine has been suggested as a possible risk factor for breast cancer, potentially through its effect of facilitating the development of benign breast disease. However, coffee and tea also contain polyphenols, which exhibit anticarcinogenic properties. A hospital-based, case-control study was conducted to evaluate the role of coffee, decaffeinated coffee, and black tea in breast cancer etiology. Study participants included 1932 cases with primary, incident breast cancer and 1895 hospital controls with nonneoplastic conditions. All participants completed a comprehensive epidemiological questionnaire. Among premenopausal women, consumption of regular coffee was associated with linear declines in breast cancer risk (P for trend = 0.03); consumers of >or=4 cups/d experienced a 40% risk reduction (odds ratio = 0.62, 95% CI 0.39-0.98). No clear associations between intake of black tea or decaffeinated coffee and breast cancer risk were noted among premenopausal women, although black tea was associated with a protective effect unique to a subsample of cases with lobular histology. Among postmenopausal women, breast cancer risk was not associated with consumption of coffee, tea, or decaffeinated coffee. Results among postmenopausal women did not differ by histologic subtype. Our findings support a protective effect of coffee intake on premenopausal, but not postmenopausal breast cancer risk. Further studies are warranted to confirm these findings. PMID:16365077

  15. Volatile compounds and sensory characteristics of various instant teas produced from black tea.

    Science.gov (United States)

    Kraujalytė, Vilma; Pelvan, Ebru; Alasalvar, Cesarettin

    2016-03-01

    Various instant teas produced differently from black tea [freeze-dried instant tea (FDIT), spray-dried instant tea (SDIT), and decaffeinated instant tea (DCIT)], were compared for their differences in volatile compounds as well as descriptive sensory analysis (DSA). A total of 63 volatile compounds in all tea samples (eight aldehydes, ten alcohols, nine ketones, five esters, eight acids, ten terpenes/terpenoids, ten furans/furanones, two pyrroles, and one miscellaneous compound) were tentatively identified. Black tea, FDIT, SDIT, and DCIT contained 60, 55, 47, and 40 volatile compounds, respectively. Ten flavour attributes such as after taste, astringency, bitter, caramel-like, floral/sweet, green/grassy, hay-like, malty, roasty, and seaweed were identified. Intensities for a number of flavour attributes (except for caramel-like in SDIT and bitter and after taste in DCIT) were not significantly different (p>0.05) among tea samples. The present study suggests that instant teas can also be used as good alternative to black tea. PMID:26471629

  16. Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons

    Directory of Open Access Journals (Sweden)

    Lin Cong

    2016-01-01

    Full Text Available Green tea polyphenols are a natural product which has antioxidative and antiapoptotic effects. It has been shown that glutamate excitotoxicity induced oxidative stress is linked to neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In this study we explored the neuroprotective effect of green teen polyphenols against glutamate excitotoxicity in the primary cultured cortical neurons. We found that green tea polyphenols protected against glutamate induced neurotoxicity in the cortical neurons as measured by MTT and TUNEL assays. Green tea polyphenols were then showed to inhibit the glutamate induced ROS release and SOD activity reduction in the neurons. Furthermore, our results demonstrated that green tea polyphenols restored the dysfunction of mitochondrial pro- or antiapoptotic proteins Bax, Bcl-2, and caspase-3 caused by glutamate. Interestingly, the neuroprotective effect of green tea polyphenols was abrogated when the neurons were incubated with siBcl-2. Taken together, these results demonstrated that green tea polyphenols protected against glutamate excitotoxicity through antioxidative and antiapoptotic pathways.

  17. Green tea for the prevention of cancer: evidence of field epidemiology

    OpenAIRE

    Min Zhang; Lin Li, Ping Liu; Holman, C. D’Arcy J.

    2012-01-01

    Background: Tea is derived from the leaf of Camellia sinensis, a natural beverage widely consumed around the world. Geological and botanical evidence suggests that the tea plant originated from China. Varying methods of processing tea leaves lead to green tea, black tea, or Oolong tea, which differ in their concentrations of polyphenols. Green tea polyphenols appear to have anti-tumorigenic properties, and form 30-40% of the dry weight of green tea compared with only 3-10% of black tea. Num...

  18. Separation of Caffeine and Tea Poly-phenols from Instant (Soluble Tea Waste Liquor by Macro-porous Resins

    Directory of Open Access Journals (Sweden)

    Xueling Gao

    2014-06-01

    Full Text Available Instant tea is presently manufactured by spraying and freeze drying the concentrated brew of processed tea leaves and dust. The purpose of this study was to develop a technique using macro-porous resins for separating Caffeine (Caf and Tea Poly-phenols (TP from the waste liquor generated by manufacturing instant tea. Optimum adsorption conditions were obtained using an initial concentration of Caf solution of 80 mg/L and a flow rate of 1.5 mL/min at 60°C; The optimal desorption conditions were determined using a flow rate of 1.0 mL/min, 80% aqueous ethanol of, eluent volume of 4 times of Bed Volume (BV. After column separation, the purity of Caf was enhanced from 6 to 23%, TP from 38 to 61% and with the final yield of 16.9% following the separation by D101 resin. This study demonstrated macro-porous resin can effectively separated Caf and TP from instant tea waste liquor.

  19. Polyphenolic Profile and Antioxidant Activities of Oolong Tea Infusion under Various Steeping Conditions

    Directory of Open Access Journals (Sweden)

    Feng Chen

    2007-11-01

    Full Text Available The phenolic profile and antioxidant activities of oolong tea extract wereinvestigated after tea was steeped in 90 or 100 oC water for 3 or 10 min. The extractionyield increased with increasing temperature and extended steeping time. However, highertemperature and longer time (100 oC water for 10 min led to loss of phenolics. Theaqueous extract of oolong tea (AEOT at 100 oC for 3 min exhibited the strongestantioxidant activity. The major polyphenolic components of the AEOT were identified as(--epigallocatechin (EGC, (--epigallocatechin gallate (EGCG and (--epicatechin-3-gallate (ECG. The two major catechins (EGC and EGCG in the tea infusion contributedsignificantly to the investigated antioxidant activities [i.e., the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH radical scavenging and superoxide radical scavengingactivities] with high correlation values in r = 0.9486 and 0.9327 for the EGC and r =0.9592 and 0.8718 for the EGCG, respectively.

  20. Green tea polyphenol epigallocatechin gallate activates TRPA1 in an intestinal enteroendocrine cell line, STC-1.

    Science.gov (United States)

    Kurogi, Mako; Miyashita, Megumi; Emoto, Yuri; Kubo, Yoshihiro; Saitoh, Osamu

    2012-02-01

    A characteristic astringent taste is elicited by polyphenols. Among the polyphenols, catechins and their polymers are the most abundant polyphenols in wine and tea. A typical green tea polyphenol is epigallocatechin gallate (EGCG). Currently, the mechanism underlying the sensation of astringent taste is not well understood. We observed by calcium imaging that the mouse intestinal endocrine cell line STC-1 responds to the astringent compound, EGCG. Among major catechins of green tea, EGCG was most effective at eliciting a response in this cell line. This cellular response was not observed in HEK293T or 3T3 cells. Further analyses demonstrated that the 67-kDa laminin receptor, a known EGCG receptor, is not directly involved. The Ca(2+) response to EGCG in STC-1 cells was decreased by inhibitors of the transient receptor potential A1 (TRPA1) channel. HEK293T cells transfected with the mouse TRPA1 (mTRPA1) cDNA showed a Ca(2+) response upon application of EGCG, and their response properties were similar to those observed in STC-1 cells. These results indicate that an astringent compound, EGCG, activates the mTRPA1 in intestinal STC-1 cells. TRPA1 might play an important role in the astringency taste on the tongue. PMID:21890837

  1. Modelling Extraction of White Tea Polyphenols: The Influence of Temperature and Ethanol Concentration

    Directory of Open Access Journals (Sweden)

    Sara Peiró

    2014-10-01

    Full Text Available The optimization of the extraction of natural antioxidants from white tea has fostered intensive research. This study has investigated the effects of ethanol-water mixtures, temperature and time on the extraction of polyphenols and antioxidant components from white tea. The response surface methodology was applied to identify the best extraction conditions. The best conditions to maximize the extraction of total polyphenols were: ethanol, 50%, for 47.5 min. Although the yield of polyphenols was optimal at 65 °C, the maximum antioxidant capacity was achieved with an extraction temperature of 90 °C. This study has identified the optimal conditions for the extraction of tea liquor with the best antioxidant properties. Epigallocatechin gallate, epicatechin gallate, epigallocatechin and epicatechin were extracted from white tea at concentrations up to 29.6 ± 10.6, 5.40 ± 2.09, 5.04 ± 0.20 and 2.48 ± 1.10 mg/100 g.

  2. DETERMINATION OF HEAVY METALS IN IRANIAN AND IMPORTED BLACK TEA

    Directory of Open Access Journals (Sweden)

    F. Ansari

    2007-09-01

    Full Text Available Tea is the most popular beverage in the world and contains several essential nutrients, which are beneficial for human health. The contamination of tea leaves by heavy metals may pose a serious threat to human, because they are not biodegradable and remain in environment and pass to food chain. In this study, the concentration of heavy metals of Cd, Pb, Ni, and Al and macro-elements of Fe, Zn, Cu, and Mn were determined by atomic absorption spectrometry on 30 samples of black tea cultivated in Iran and compared with the results for 30 samples of imported black tea in 2006. The results of analysis showed that the mean level of Al was 699.2±172.7mg/kg for Iranian and 388.3±98.3mg/kg for imported black tea. However, the values for Cd, Pb, and Ni were non-detectable. The most abundant nutritive metal was manganese with 155.2-214.2mg/kg and 96.7-332.9mg/kg in Iranian and imported black tea, respectively. The average contents of detectable heavy metals were significantly (p<0.05 higher in Iranian black tea. According to the results of this study, it is justifiable to set maximum residue level for heavy metals in tea, such as Al which appears to be very high in concentration.

  3. New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate

    Directory of Open Access Journals (Sweden)

    Hae-Suk Kim

    2014-01-01

    Full Text Available Green tea is rich in polyphenol flavonoids including catechins. Epigallocatechin 3-gallate (EGCG is the most abundant and potent green tea catechin. EGCG has been extensively studied for its beneficial health effects as a nutriceutical agent. Based upon its chemical structure, EGCG is often classified as an antioxidant. However, treatment of cells with EGCG results in production of hydrogen peroxide and hydroxyl radicals in the presence of Fe (III. Thus, EGCG functions as a pro-oxidant in some cellular contexts. Recent investigations have revealed many other direct actions of EGCG that are independent from anti-oxidative mechanisms. In this review, we discuss these novel molecular mechanisms of action for EGCG. In particular, EGCG directly interacts with proteins and phospholipids in the plasma membrane and regulates signal transduction pathways, transcription factors, DNA methylation, mitochondrial function, and autophagy to exert many of its beneficial biological actions.

  4. Electronic Nose for Black Tea Quality Evaluation Using Kernel Based Clustering Approach

    OpenAIRE

    Ashis Tripathy; Mohanty, A.K.(Bhabha Atomic Research Centre, Mumbai, India); Mihir Narayan Mohanty

    2012-01-01

    Black Tea is conventionally tested by human sensory panel called “Tea Tasters”, who assign quality scores to different teas. In this paper electronic nose based evaluation of black tea samples have been described. One of the principal problems encountered in the above studies is collection of tea samples. These tea industries in India are spread over dispersed locations and quality of tea varies considerably on agroclimatic condition, type of plantation, season of flush and method of manufact...

  5. Tea and cinnamon polyphenols improve the metabolic syndrome

    Science.gov (United States)

    Hyperglycemia and insulin resistance, key features of the metabolic syndrome, are leading causes of early brain alterations. Green tea, by a mechanism involving the improvement of insulin sensitivity and the control of oxidative stress, could be a factor of neuroprotection in insulin-resistant state...

  6. Degradation of bromothymol blue by 'greener' nano-scale zero-valent iron synthesized using tea polyphenols

    Science.gov (United States)

    A green single-step synthesis of iron nanoparticles using tea (Camellia sinensis) polyphenols is described that uses no added surfactants/polymers as a capping or reducing agents. The expeditious reaction between polyphenols and ferric nitrate occurs within few minutes at room te...

  7. Sri Lankan black tea (Camellia sinensis L.) inhibits the methylglyoxal mediated protein glycation and potentiates its reversing activity in vitro

    Institute of Scientific and Technical Information of China (English)

    Wanigasekara Daya Ratnasooriya

    2016-01-01

    Objective: To evaluate inhibitory activity of methylglyoxal (MGO) mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea. Methods: Freeze dried black tea brew (BTB) was used as the sample in this study. Anti-glycation and glycation reversing activity was studied in bovine serum albumin (BSA)-MGO model. Antioxidant properties were studied using total polyphenolic content, total flavonoid content, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays. Results: The results demonstrated significant (P Conclusions: The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.

  8. Polyphenolic rich traditional plants and teas improve lipid stability in food test systems.

    Science.gov (United States)

    Ramsaha, Srishti; Aumjaud, B Esha; Neergheen-Bhujun, Vidushi S; Bahorun, Theeshan

    2015-02-01

    The deleterious effects of lipid autoxidation are of major concern to the food industry and can be prevented by food antioxidants. In this vein, the phenolic contents and antioxidant potential of traditional plants of Mauritius such as P. betle L. (Piperaceae), M. koenigii L. Sprengel. (Rutaceae), O. gratissimum L. (Lamiaceae), O. tenuiflorum L. (Lamiaceae), and commercially available Mauritian green and black teas were evaluated. Their ferric reducing antioxidant power (FRAP) were compared to that of butylated hydroxytoluene (BHT) with the following order of potency: BHT > "Natural" commercial green tea > "Black Label" commercial black tea > O. gratissimum > P. betle > O. tenuiflorum > M. koenigii. The trolox equivalent antioxidant capacity (TEAC) assay reflected a similar antioxidative order for BHT and "Natural" commercial green tea, with however P. betle, O. tenuiflorum and O. gratissimum exhibiting higher activities than "Black Label" commercial black tea and M. koenigii. Based on their potent antioxidant capacity, P. betle (0.2 % m/m) and O. tenuiflorum (0.2 % m/m) extracts, and green tea (0.1 % m/m) infusate were compared with BHT (0.02 % m/m) on their ability to retard lipid oxidation in unstripped sunflower oil and mayonnaise during storage at 40 °C. P. betle and green tea were more effective than BHT in both food systems. Moreover, odour evaluation by a sensory panel showed that the plant extracts and green tea infusate effectively delayed the development of rancid odours in unstripped sunflower oil and mayonnaise (p < 0.05). PMID:25694685

  9. ESR detection of free radicals in polyphenolic extracts from wine grapes, olives and green tea

    International Nuclear Information System (INIS)

    Full text: Polyphenols are widespread in vegetables and fruits and they play an important role in human diet and health: these compounds act mainly as antioxidants and radical scavengers. In this work we have detected free radicals in the following natural polyphenols: Endotelon, an anthocyanic grapeskin extract; malvidin 3,5-O-diglucosides (malvin); oleuropein, an olive polyphenol; a commercial green tea extract, and pure epigallocatechingallate EGCG. The investigation was performed using a Varian E-12 ESR Spectrometer (∼9.1 GHz) at room temperature. All except the green tea extract gave single unstructured lines of ∼ 10 gauss linewidth. The tea extract signal showed 3 lines, one ∼ 20 gauss wide, one ∼ 10 gauss wide, and one ∼ 2-3 gauss wide. Saturation behaviour of these lines at room and liquid N2 temperature showed them to be from different radicals About 50% of the extract is represented by epigallocatechingallate (EGCG). Using the pure sample EGCG it was possible to assign the appropriate radical, which corresponded with the broadest and strongest 'single' signal (∼20g wide). The presence of the free radicals in the solid extracts shows that the appropriate molecules can act as radical scavengers by forming stable radicals

  10. Polyphenol Content and Antioxidant Capacity of Herbal Tea from Vietnamese Water Hyssop (Bacopa monnieri

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    Phan-Tai Huan

    2016-01-01

    Full Text Available A sustainable process to produce herbal tea from Vietnamese water hyssop (Bacopa monnieri has been conducted. The product can be used as traditional Ayuvedic medicinal herb. In this study, factors affecting drying process have been investigated including temperature ranging from 60 to 80°C, material thickness from 0.18 to 0.54 g/cm2 and air velocity from 0.5 to 1.5 m/s. Total polyphenols and soluble solid contents were observed during the process. Antioxidant capacity of raw and dried water hyssop have been also evaluated. The optimum result showed that at temperature of 60°C, material thickness of 0.18 g/cm2 and air velocity of 1.5 m/s, polyphenols content and total soluble solids in final herbal tea were found at 15.13 mg/g gallic acid equivalent and 4.00%. With saponins concentrations of 0.53% and 0.50%, the IC50 values of 3.94 µg/ml and 4.45 µg/ml were found in the raw and dried water hyssop tea, respectively. It’s required to dry water hyssop at optimum condition in 180 minutes and chop materials into 1mm lengths before being used as herbal tea.

  11. Consumption of Green Tea, but Not Black Tea or Coffee, Is Associated with Reduced Risk of Cognitive Decline

    OpenAIRE

    Noguchi-Shinohara, Moeko; Yuki, Sohshi; Dohmoto, Chiaki; Ikeda, Yoshihisa; Samuraki, Miharu; Iwasa, Kazuo; Yokogawa, Masami; Asai, Kimiko; Komai, Kiyonobu; Nakamura, Hiroyuki; Yamada, Masahito

    2014-01-01

    Our objective was to determine whether the consumption of green tea, coffee, or black tea influences the incidence of dementia and mild cognitive impairment (MCI) in older people. We conducted a population-based prospective study with Japanese residents aged >60 years from Nakajima, Japan (the Nakajima Project). Participants received an evaluation of cognitive function and blood tests. The consumption of green tea, coffee, and black tea was also evaluated at baseline. Of 723 participants with...

  12. Simultaneous determination of caffeine and some selected polyphenols in Wuyi Rock tea by high-performance liquid chromatography.

    Science.gov (United States)

    Zhao, Feng; Lin, He-Tong; Zhang, Shen; Lin, Yi-Fen; Yang, Jiang-Fan; Ye, Nai-Xing

    2014-04-01

    The primary taste and healthy benefits of tea are mainly attributed to tea polyphenols and caffeine. Due to very many kinds of flavonoid glycosides in tea and the lack of commercial standards of flavonoid glycosides, it is critical to develop a rapid and cheap method for determining flavonoid glycosides of tea. Contents of myricetin glycosides and quercetin glycosides in Wuyi Rock tea were determined by detecting contents of corresponding myricetin and quercetin. Optimizing hydrolysis conditions for hydrolyzing flavonoid glycosides to their corresponding flavonols including quercetin and myricetin in Wuyi Rock tea was a key technology for detecting contents of corresponding myricetin and quercetin. The results showed that hydrolysis at 2 mol/L HCl solution and at 90 °C for 1 h was an optimizing condition for hydrolyzing flavonoid glycosides to myricetin and quercetin in Wuyi Rock tea. Caffeine and seven kinds of polyphenols (GA, EGC, C, EGCG, EC, ECG, and CGA) in 20 samples of Wuyi Rock tea were simultaneously determined using a simple and fast reverse-phase high-performance liquid chromatography procedure coupled with photodiode array detector (RP-HPLC-PDAD). The results indicated that there were significant (P < 0.05) differences of ECG, CGA, ECG, and myricetin glycosides in 'Wuyi Rougui' and 'Wuyi Shuixian', which were credited with causing the difference in taste between these two cultivar of Wuyi Rock tea. The study may be useful for clarifying the cause of "cultivated varieties flavor" of Wuyi Rock tea. PMID:24625357

  13. Characterization of Black and Green Tea from Local Market

    Directory of Open Access Journals (Sweden)

    Sonia Ancuta Socaci

    2013-11-01

    Full Text Available The leaves from Camellia sinensis are used from ancient times for preparation of tea but also as raw material for different extracts which are used in food industry as well as in pharmaceutical or cosmetic products. Due to the increasing interest in tea health benefits, the aim of the present study was to characterize several brands of green and black tea found on local market, regarding their content in total phenolic compounds, flavonoids and antioxidant capacity. Total phenolics and flavonoids were determined spectrophotometrically using a modified Folin-Ciocalteu method, respectively a chromogenic system of NaNO2–Al(NO33–NaOH based method. The antioxidant capacity of each tea sample was assessed through the evaluation of free radical scavenging effect on 2,2-diphenyl-1-picrylhydrazyl. The results obtained for the green and black tea samples varied widely, depending on the tea variety. The antioxidant capacity of the analyzed teas ranged between 12.10 and 40.03%RSA, while the total phenolic content was within 2090 and 6080 mg GA/ 100g. The concentrantion in flavonoids was between 9.04 and 15.34 g/100g of tea.

  14. Green tea polyphenols as inhibitors of ribonuclease A

    International Nuclear Information System (INIS)

    Ribonucleases (RNases), which are essential for cleavage of RNA, may be cytotoxic due to undesired cleavage of RNA in the cell. The quest for small molecule inhibitors of members of the ribonuclease superfamily has become indispensable with a growing number exhibiting unusual biological properties. Thus, inhibitors of RNases may serve as potential drug candidates. Green tea catechins (GTC), particularly its major constituent (-)-epigallocatechin-3-gallate (EGCG), have reported potential against cell proliferation and angiogenesis induced by several growth factors including angiogenin, a member of the RNase superfamily. This study reports the inhibition of bovine pancreatic ribonuclease A (RNase A) by EGCG and GTC. This has been checked qualitatively by an agarose gel based assay. Enzyme kinetic studies with cytidine 2',3' cyclic monophosphate as the substrate have also been conducted. Results indicate substantial inhibitory activity of a noncompetitive nature with an inhibition constant of ∼80 μM for EGCG and ∼100 μM for GTC measured in gallic acid equivalents

  15. Green Tea Polyphenols Reduce Body Weight in Rats by Modulating Obesity-Related Genes

    OpenAIRE

    Chuanwen Lu; Wenbin Zhu; Chwan-Li Shen; Weimin Gao

    2012-01-01

    Beneficial effects of green tea polyphenols (GTP) against obesity have been reported, however, the mechanism of this protection is not clear. Therefore, the objective of this study was to identify GTP-targeted genes in obesity using the high-fat-diet-induced obese rat model. A total of three groups (n = 12/group) of Sprague Dawley (SD) female rats were tested, including the control group (rats fed with low-fat diet), the HF group (rats fed with high-fat diet), and the HF+GTP group (rats fed w...

  16. Effect of types of extractions on antioxidant activity of varieties of black teas from India

    Directory of Open Access Journals (Sweden)

    Hafeeza Khanum

    2015-02-01

    Full Text Available Tea sample (silver cloud dust was extracted with water by traditional extraction method (TEM at variable material: solvent ratio (1:20, 1:30, 1:40, 1:50, temperature (50, 60, 70, 80, 90˚C and time (10, 20, 30, 40 mins. Extracts prepared were analysed for antioxidant activity (AA and total polyphenol content (TPP. The extract of 1:50, 80˚C, 30 mins showed higher yield , TPP and RSA compared to other extracts. Extraction (1:50, 80˚C for 10,20,30,40 mins was also carried by using magnetic stirring and sonicator. TEM showed higher AA and TPP. Therefore different samples of tea dust and leaf were extracted by TEM. All black tea leaf showed higher caffeine content than dust. Darjeeling leaf exhibited higher yield, RSA, TPP and caffeine. TF ranged between 0.1-0.2 % while TR content was 3.8-6% for varieties of tea extracts. Nilgiri dust extract showed higher colour. Darjeeling leaf showed highest caffeine content (12%.

  17. Therapeutic Response of Black Tea Extract on Maintenance Pancreas and Intestine of Gamma-irradiated Rats

    International Nuclear Information System (INIS)

    To protect animals health from hazards caused by acute exposure to environmental hazardous viz., ionising-radiation (γ-rays), it is recommended that antioxidants could be taken regularly in nutrition. Black tea (Camellia sinensis) has received a great deal of attention because tea polyphenols are strong antioxidants. Possible ameliorative actions of black tea extracts (BTE) were examined at the pancreas and intestinal levels, which are sensitive targets for radiation damage following whole body γ-irradiation. Plasma antioxidant status measured as ferric-reducing antioxidant power (FRAP), intestine marker enzyme activity: Xanthine oxidase system (XO), serum and pancreatic damage markers viz., lipase and amylase, oxidative stress marker in pancreas and intestine viz., thiobarbituric acid reactive substances (TBARS), pancreatic and intestinal total glutathione (GSH) and activity of antioxidant enzymes viz., superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT). All above parameters were measured in four different groups of rats, namely control, tea-rats, irradiated-rats, irradiated-tea rats. Results of irradiated rats showed that, plasma level of FRAP was decreased significantly but, serum lipase and amylase activities were increased. Pancreatic lipase, amylase, GSH and antioxidant enzyme activities were decreased significantly. However, TBARS was increased. Intestinal XO and TBARS levels were significantly increased but GSH and antioxidant enzymes levels were decreased. Drinking BTE prevents largely the changes occurred in all measured parameters investigated in plasma, serum, pancreas and intestine. These findings suggest that BTE modulate pancreatitis and intestine damages caused by acute 7 Gy γ- rays toxicity presumably by enhancing antioxidant status and inhibiting oxidative stress. Conclusion: BTE could normalise γ-rays-induced suppression of activities of pancreatic and intestinal tissues

  18. Chitosan green tea polyphenol complex as a released control compound for wound healing

    Institute of Scientific and Technical Information of China (English)

    QIN Yao; WANG Hong-wei; Thirupathi Karuppanapandian; Wook Kim

    2010-01-01

    Objective: In recent years, oxidative stress has been implicated in a variety °enerative pro-cess and diseases, including acute and chronic inflamma-tory conditions such as wound healing.Green tea polyphe-nols have shown anti-oxidant property.The present study discussed the application of chitosan green tea polyphenol complex on the wound healing.Methods: The wound healing effect ofchitosan green tea polyphenol complex was studied in ten-week-old healthy male Sherman rats weighing 150-180 g by two wound models.The rats were randomly chosen and divided into four groups (n=5), administered with distilled water in Group A as con-trol group, epigallocatechin-3-gallate (EGCG) in Group B, chitosan-EGCG complex in Group C and chitosan-green tea polyphenols complex in Group D, respectively.In rats'incision wound model, two straight paravertebral inci-sions were made and skin tensile strength was measured using continuous water flow technology on the 10th day.In rats'excision wound model, wound contraction and pe-riod of epithelization were measured.The polyphenols re-lease from the complex was continuously monitored by an elution technique in aqueous solution at different pH val-ues (pH=4, 5, 6, 7).Results: The treatment groups showed significantly enhanced the breaking strength in incision wound (328±4.5) g and (421±18.5) g compared with control (264±16.7) g.In the excision wound model, the wound contraction percentage in treatment groups was relatively increased during the re-covery period.Respectively, the percentage of wound contraction ranged from 47.60%±2.15% on day 4 to 107.98% ±1.26% on day 16 compared with control group (8.46%±5.42% to 59.80%±4.47%).The complex demonstrated a gradual in-crease in the release rate from the initial stage and slow increase at different pH values.The release rate approxi-mated 0.6-0.7 in the complex and remained stable 6 hours after injury, which may be the end of the release process.Conclusions: In our study, chitosan

  19. Anticariogenic Activity of Black Tea - An Invivo Study

    Science.gov (United States)

    Arya, Vishal; Srivastava, Ankit; Nandlal, Swati

    2016-01-01

    Introduction Teas is known for its anticariogenic properties and various mechanisms have been invoked to explain this effect. One such proposed mechanism is inhibition of salivary alpha amylase activity by endogenous tannins present in tea. Aim The objective of the present study was to determine whether or not the ingestion of black tea decoction inhibits the enzyme salivary amylase and thus interferes with the release of maltose from intraoral entrapped particles of food. Materials and Methods A total of 30 children in the age group of 12 - 15 years were selected for the study. After two hours of fasting subjects consumed two salted crackers for 60 second following which they rinsed with water (control solution) and then with 1.5% black tea decoction (test solution) next day. Retained food particles were recovered from buccal aspect of left mandibular premolar and salivary amylase activity was noted via chromatography. Paired t-test was applied for statistical analysis. Results Maltose to Sucrose ratio was used to evaluate the result. The average ratio was 3.27 for control solution and 1.82 for test solution. The results were statistically highly significant (p <0.005). Conclusion Tea inhibited the activity of salivary amylase and this inhibition assumes a special significance when it is considered that the effect of tea could be manifested over a prolonged period of time, as in a real life situation. PMID:27135007

  20. ``Green'' functionalization of magnetic nanoparticles via tea polyphenol for magnetic resonance/fluorescent dual-imaging

    Science.gov (United States)

    Jiang, Wen; Lai, Kuilin; Liu, Kexia; Xia, Rui; Gao, Fabao; Wu, Yao; Gu, Zhongwei

    2014-01-01

    Tea polyphenol serves as an environmentally friendly ligand-exchange molecule to synthesize multifunctional metal-doped superparamagnetic iron oxide nanoparticles via a catechol-metal coordination interaction. The resultant particles not only exhibit excellent hydrophilicity and protein adsorption resistance, but also are applicable as magnetic resonance/fluorescent dual-imaging probes due to their high T2 relaxivity, autofluorescence and large cellular uptake.Tea polyphenol serves as an environmentally friendly ligand-exchange molecule to synthesize multifunctional metal-doped superparamagnetic iron oxide nanoparticles via a catechol-metal coordination interaction. The resultant particles not only exhibit excellent hydrophilicity and protein adsorption resistance, but also are applicable as magnetic resonance/fluorescent dual-imaging probes due to their high T2 relaxivity, autofluorescence and large cellular uptake. Electronic supplementary information (ESI) available: Additional information and figures (Fig. S1-S7), including experimental sections, characterization of the products, protein corona analysis, cytotoxicity and cellular uptake quantification. See DOI: 10.1039/c3nr05003c

  1. Molecular Mechanisms and Biological Implications of Green Tea Polyphenol (--Epigallocatechin -3- gallate

    Directory of Open Access Journals (Sweden)

    Subramani C

    2013-07-01

    Full Text Available Plants constitute a vital part of the biological system in which we exist and are indispensable to humans in every aspect of life. Its major percentage of naturally occurring material used as drugs by people all over the world. The use of plants as food and medicines when man started his life on this planet. Now people started to realize the value of plants as drugs after they felt the serious side effects of modern medicine. The practice of wide usage of plants as medicinal agents is prevalent for centuries in various parts of the world, the most prominent of which are Chinese system of medicine, ayurvedic, siddha and unani system of medicine in India. The realization that there is something interesting in the properties of these medicinal plants dawned with the advent of chemistry in the late 18th century. Chemists gradually started isolating pure substances from various anatomical parts of medicinal plants and concluded that certain active compounds are responsible for the therapeutic actions of the plants. It has been estimated that only 10-15 % of 2,50,000 to 7,50,000 existing species of higher plants have been surveyed for biologically active compounds for the study of its phytochemistry and pharmacology. This review paper deals with the advances in study of green tea, which is the second most consumed beverage in the Worldafter water. Green tea catechins are the flavan-3-ols found in green tea leaves, of the catechins. Out of the various catechins found in green tea, (--Epigallocatechin-3-gallate (EGCG is the most abundant one in green tea leaves. It is the most potent antioxidant polyphenols associated with anti-atherogenic, antitumor and anti-mutagenic activities.

  2. Antioxidant-mediated preventative effect of Dragon-pearl tea crude polyphenol extract on reserpine-induced gastric ulcers

    OpenAIRE

    YI, RUOKUN; Wang, Rui; Sun, Peng; Zhao, Xin

    2015-01-01

    Dragon-pearl tea is a type of green tea commonly consumed in Southwest China. In the present study, the antioxidative and anti-gastric ulcer effects of Dragon-pearl tea crude polyphenols (DTCP) were determined in vitro and in vivo. Treatment with 25, 50 or 100 µg/ml DTCP resulted in notable antioxidant effects in vitro, which manifested as 2,2-diphenyl-1-picrylhydrazyl and OH radical-scavenging activity. Furthermore, using an in vivo mouse model, DTCP was shown to reduce the gastric ulcer are...

  3. Comparison of the Level of Boron Concentrations in Black Teas with Fruit Teas Available on the Polish Market

    Directory of Open Access Journals (Sweden)

    Anetta Zioła-Frankowska

    2014-01-01

    Full Text Available The determination of boron by inductively coupled plasma-atomic emission spectrometry has been carried in water-soluble and acid soluble (total content fractions of 36 samples of traditional black tea and fruit brew. The estimation of the impact of the type of tea on the concentration of boron in water-soluble and acid extracts and potential human health risk from the daily intake of boron was carried out in this study. The levels of boron differed significantly in black and fruit tea types. The mean total content of boron ranged from 8.31 to 18.40 mg/kg in black teas, from 12.85 to 15.13 mg/kg in black tea with fruit flavor, and from 12.09 to 22.77 mg/kg in fruit brews. The degree of extraction of boron in black tea ranged from 8% to 27% and for fruit tea from 17% to 69%. In addition, the values below 25% were of black teas with fruit flavors. The daily intake of B from tea infusions (three cups/day is still within the average daily intake except for some of the fruit brews which exceed acceptable regulations of the daily intake of total boron by humans. Hence, it may not produce any health risks for human consumption, if other sources of metal contaminated food are not taken at the same time.

  4. Effects of gamma radiation on sensorial properties in black tea

    International Nuclear Information System (INIS)

    The black tea (Camellia sinensis) is the most consumed beverage in the world and its consumption has increased, manly in occidental countries, due to the fact that this drink has large antioxidants quantities. In this type of tea, the taste determines the product qualities and its final value. Several studies reported that food irradiation is an excellent process to disinfect food, reducing storage losses and extended its shelf life. This treatment can inhibit cellular division, promoting a molecular and DNA structural modification. Depending on the dose applied, the irradiation can modify sensorial properties, influencing the marked cost. The sensorial analysis is one of the main tests to detect any better or worse changes, by consumers. The aim of this study is to evaluate the effect of ionizing radiation on sensorial properties in black tea. Samples will be irradiated with a 60Co source, at doses of 0, 5, and 10 kGy. (author)

  5. Production, Composition, and Health Effects of Oolong Tea

    Directory of Open Access Journals (Sweden)

    İlkay Koca

    2014-03-01

    Full Text Available Tea, one of the most widely consumed beverages in the world, is produced from the leaves of the plant Camellia sinensis L.. Tea has important physiological properties and potential health benefits due to the presence of compounds such as polyphenols, amino acids, vitamins, carbohydrates, caffeine, and purine alkaloids. Tea is produced in three types as green tea (unfermented, oolong tea (partially fermented, and black tea (fully fermented. Black tea is consumed worldwide, whereas green and oolong teas are consumed mainly in Asia and North Africa. The total tea production in the world consists of about 78% black tea, 20% green tea and <2% oolong tea. In the production of green tea, the fresh leaves are steamed or roasted to avoid enzymatic oxidation. Black tea is fully oxidized during fermentation. Oolong tea is partially fermented to permit a moderate level of enzymatic oxidation during processing. The degree of fermentation of oolong tea leaves ranges from 20 to 60%, depending upon consumer demand. Oolong tea has a taste and color somewhere between green and black tea. Green tea contains 30-42% catechins in dry mass, while black tea contains 3-10% and oolong 8-20%. Oolong tea has a higher antioxidant activity and lipoxygenase inhibitory activity as compared to black tea. Oolong tea exhibits a stronger antimutagenic activity than green or black tea. Oolong tea is reported to have anticancer, antiobesity, antidiabetes, antiallergic effects, and prevent atherosclerosis and heart disease. In this review, the production, composition and health effects of oolong tea have been discussed.

  6. Development of LC-MS/MS method for analysis of polyphenolic compounds in juice, tea and coffee samples

    Science.gov (United States)

    A simple and fast method for the analysis of a wide range of polyphenolic compounds in juice, tea, and coffee samples was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was based on a simple sample preparation “dilute and shoot” approach, and LC-MS/MS triple qu...

  7. Protective actions of green tea polyphenols and alfacalcidol on bone microstructure in female rats with chronic inflammation

    Science.gov (United States)

    This study investigated the effects of green tea polyphenols (GTP) and alfacalcidol on bone microstructure and strength along with possible mechanisms in rats with chronic inflammation. A 12-week study using a 2 (no GTP vs. 0.5%, w/v GTP in drinking water) × 2 (no alfacalcidol vs. 0.05 ug/kg alfacal...

  8. Tea extracts antioxidative potential in emulsified lipid systems

    OpenAIRE

    Anna Gramza-Michałowska; Marzenna Hęś; Józef Korczak

    2008-01-01

    Tea leaves (Camelia sinensis L.) extracts are source of polyphenols, i.e. antioxidant components. Research showed possible tea extracts use in food technology, influencing contained lipids stability improvement. The aim of the research was comparison of different teas extracts activity in emulsified lipid system. The present research examined different teas: white, green, yellow, oolong and black aqueous and ethanol extracts. To evaluate the most potent addition level different tea extracts c...

  9. Polyphenolic rich traditional plants and teas improve lipid stability in food test systems

    OpenAIRE

    Ramsaha, Srishti; Aumjaud, B. Esha; Vidushi S. Neergheen-Bhujun; Bahorun, Theeshan

    2013-01-01

    The deleterious effects of lipid autoxidation are of major concern to the food industry and can be prevented by food antioxidants. In this vein, the phenolic contents and antioxidant potential of traditional plants of Mauritius such as P. betle L. (Piperaceae), M. koenigii L. Sprengel. (Rutaceae), O. gratissimum L. (Lamiaceae), O. tenuiflorum L. (Lamiaceae), and commercially available Mauritian green and black teas were evaluated. Their ferric reducing antioxidant power (FRAP) were compared t...

  10. Green tea polyphenol epigallocatechin-3-gallate suppresses melanoma growth by inhibiting inflammasome and IL-1{beta} secretion

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, Lixia Z.; Liu, Weimin; Luo, Yuchun; Okamoto, Miyako; Qu, Dovina; Dunn, Jeffrey H. [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States); Fujita, Mayumi, E-mail: mayumi.fujita@ucdenver.edu [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States); Denver Veterans Affairs Medical Center, Denver, CO 80220 (United States)

    2011-10-28

    Highlights: Black-Right-Pointing-Pointer EGCG inhibits melanoma cell growth at physiological doses (0.1-1 {mu}M). Black-Right-Pointing-Pointer EGCG inhibits melanoma cell growth via inflammasomes and IL-1{beta} suppression. Black-Right-Pointing-Pointer Inflammasomes and IL-1{beta} could be potential targets for future melanoma therapeutics. -- Abstract: Epigallocatechin-3-gallate (EGCG), the major polyphenolic component of green tea, has been demonstrated to possess anti-inflammatory, antioxidant, anti-mutagenic and anti-carcinogenic properties. The anti-melanoma effect of EGCG has been previously suggested, but no clear mechanism of action has been established. In this study, we demonstrated that EGCG inhibits melanoma cell growth at physiological doses (0.1-1 {mu}M). In the search for mechanisms of EGCG-mediated melanoma cell suppression, we found that NF-{kappa}B was inhibited, and that reduced NF-{kappa}B activity was associated with decreased IL-1{beta} secretion from melanoma cells. Since inflammasomes are involved in IL-1{beta} secretion, we investigated whether IL-1{beta} suppression was mediated by inflammasomes, and found that EGCG treatment led to downregulation of the inflammasome component, NLRP1, and reduced caspase-1 activation. Furthermore, silencing the expression of NLRP1 abolished EGCG-induced inhibition of tumor cell proliferation both in vitro and in vivo, suggesting a key role of inflammasomes in EGCG efficacy. This paper provides a novel mechanism for EGCG-induced melanoma inhibition: inflammasome downregulation {yields} decreased IL-1{beta} secretion {yields} decreased NF-{kappa}B activities {yields} decreased cell growth. In addition, it suggests inflammasomes and IL-1{beta} could be potential targets for future melanoma therapeutics.

  11. Mineral Analysis the Infusion of Black Tea Samples by Atomic Absorption Spectrometry

    OpenAIRE

    Lahiji N.; Tadayon F.; Tamiji F.; Lahiji A. H.

    2013-01-01

    Tea infusion is one of the most popular drinks around the world. Since tea infusion is known to contain several essential nutrients, it is considered a healthy beverage. In this study eight different Iranian brands of tea infusion and eleven brands imported tea infusion samples from another country for Cu, Zn, Mn and Al were determined by flame atomic absorption spectrometry after wet digestion. The results of analysis showed that the extraction rates of minerals from dry black tea to infusio...

  12. Influence of green tea polyphenols on mitochondrial permeability transition pore and Ca2+ transport

    Institute of Scientific and Technical Information of China (English)

    沈生荣; 于海宁; 金超芳

    2002-01-01

    The authors investigated the influence of green tea polyphenols(GPSs) on the liver mitochondria permeability transition pore(PTP) opening through mitochondria sweling and change of mitochcodrin membrane potetial .The data showed that GPSs had obvious protective effect on the Ca2+ -induced PTP opening in a dose-dependent manner detected by mitochondria swelling.The results were obtained by meating the change of mitochondria membrane potential through Rh 123.Further experiments were conducted to examine the detailed influence of GTPs on Ca2+ import and export of mitochondria.The results showed that GTPs had remarkably inhibitory effect on the Ca2+-induced Ca2+ import in mitochondria.and they could accelerate Ca2+ -release from from mitochondria.Our data provide an altemate interpretation of the potent protective function of GPSs on cell against apoptosis.

  13. Influence of green tea polyphenols on mitochondrial permeabi lity transition pore and Ca2+ transport

    Institute of Scientific and Technical Information of China (English)

    沈生荣; 于海宁; 金超芳

    2002-01-01

    The authors investigated the influence of green tea polyphenols (GTPs) on the liver mitochondria permeability transition pore (PTP) opening through mit ochondria swelling and change of mitochondria membrane potential. The data showe d that GTPs had obvious protective effect on the Ca2+-induced PTP opening in a d ose-dependent manner detected by mitochondria swelling. The results were obtain ed by measuring the change of mitochondria membrane potential through Rh 123. Further experiments were conducted to examine the detailed influence of GTP s on Ca2+import and export of mitochondria. The results showed that GTPs h ad rem arkably inhibitory effect on the Ca2+-induced Ca2+ import in mitoch ondria; and t hat they could accelerate Ca2+-release from mitochondria. Our data provid e an al ternate interpretation of the potent protective function of GTPs on cell against apoptosis.

  14. Pomegranate and mint syrup addition to green tea beverage stabilized its polyphenolic content and biofunctional potentials during refrigerated storage.

    Science.gov (United States)

    Dhaouadi, Karima; Belkhir, Manel; Raboudi, Faten; Mecha, Elsa; Ghommeme, Imen; Bronze, Maria Do Rosario; Ammar, Hajer; Fattouch, Sami

    2016-02-01

    The chemical stability of the green tea (GT) preparation during refrigerated storage was investigated following the addition of mint (MS) or pomegranate (PS) syrups, a common habit in the Mediterranean countries that improves the savor of this popular beverage. The supernatants recovered by centrifuging GT supplemented or not with mint (GTMS) or pomegranate (GTPS) syrup were examined for their polyphenolic profiles using the high performance liquid chromatography with diode array detection and electrospray ionization-mass spectrometry. Following storage at 4 °C for 15 days, not-supplemented GT showed a significant decrease (≈92 %) of its phenolic content. However, the decrease was relatively lesser in GTPS (≈36 %) and in GTMS (≈40 %). The observed slight increase of the extractable polyphenolics in PS and MS during the storage might explain in part the relatively limited decrease of GTPS and GTMS total phenolic content. However, chromatographic examination proved that some tea compounds, particularly caffeine, were preserved following PS and MS supplementation. Likewise, syrups'addition to GT significantly (P syrups supplementation noticeably preserved the tea bacteriostatic and bactericide activities during storage. The obtained analytical results demonstrate that MS or PS addition to green tea beverage stabilized its polyphenolic content and biofunctional properties during refrigerated storage, thus, scientifically supporting this popular practice in the Mediterranean countries. PMID:27162396

  15. Green tea polyphenols rescue of brain defects induced by overexpression of DYRK1A.

    Directory of Open Access Journals (Sweden)

    Fayçal Guedj

    Full Text Available Individuals with partial HSA21 trisomies and mice with partial MMU16 trisomies containing an extra copy of the DYRK1A gene present various alterations in brain morphogenesis. They present also learning impairments modeling those encountered in Down syndrome. Previous MRI and histological analyses of a transgenic mice generated using a human YAC construct that contains five genes including DYRK1A reveal that DYRK1A is involved, during development, in the control of brain volume and cell density of specific brain regions. Gene dosage correction induces a rescue of the brain volume alterations. DYRK1A is also involved in the control of synaptic plasticity and memory consolidation. Increased gene dosage results in brain morphogenesis defects, low BDNF levels and mnemonic deficits in these mice. Epigallocatechin gallate (EGCG - a member of a natural polyphenols family, found in great amount in green tea leaves - is a specific and safe DYRK1A inhibitor. We maintained control and transgenic mice overexpressing DYRK1A on two different polyphenol-based diets, from gestation to adulthood. The major features of the transgenic phenotype were rescued in these mice.

  16. Ameliorative effect of black tea on nicotine induced cardiovascular pathogenesis in rat

    OpenAIRE

    Gholamhoseinian, Ahmad; Joukar, Farzin; Joukar, Siyavash; Najafipour, Hamid; Shahouzehi, Beydolah

    2012-01-01

    Regarding the role of nicotine in the development of cardiovascular complications of smoking, we investigated whether black tea has a modulatory effect on cardiovascular pathogenesis of nicotine in rat. Animals were randomized to control, tea, nicotine and tea plus nicotine groups. Test groups received black tea brewed (adding 400 ml boiling water to 10 g Lipton black tea for 5 min) orally alone or with nicotine 2 mg/kg/day, s.c. separately or combined for four weeks. On 28th day, lipids p...

  17. Study of tea polyphenol as a reversal agent for carcinoma cell lines' multidrug resistance (study of TP as a MDR reversal agent)

    International Nuclear Information System (INIS)

    The aim of this study was to examine MDR1 expression product P-glycoprotein (Pgp) and study the effect and mechanism of tea polyphenol (TP) in reversion of multidrug resistance (MDR) in carcinoma cell lines. Immunocytochemical method was used for qualitative detection of Pgp. A comparative study of cytotoxicity and multidrug resistance reversion effect was made by MTT assay for tea polyphenol and quinidine in MCF-7 and MCF-7/Adr cell lines. The multidrug resistance reversion effect and mechanism were studied by measuring the uptake of 99mTc-tetrofosmin in the carcinoma cell lines. (1) The Pgp overexpression in MCF-7/Adr cells was found to be strong positive, while the Pgp expression of MCF-7 was negative. (2) Although both tea polyphenol and quinidine could not remarkably change the toxicity of adriamycin to MCF-7, they could improve the sensitivity of MCF-7/Adr to adriamycin. The reversion index of tea polyphenol and quinidine was 3 and 10 respectively. (3) The cellular uptake of 99mTc-tetrofosmin was remarkably lower in MCF-7/Adr than in MCF-7. The uptake of 99mTc-tetrofosmin in MCF-7/Adr exhibited a 4, 13, 16 fold increase in the presence of 200, 400 and 500 μg/ml of tea polyphenol respectively. The uptake of 99mTc-tetrofosmin in MCF-7/Adr exhibited only a 4-fold increase in the presence of 200 μM of quinidine. Immunocytochemistry can detect P-glycoprotein expression level qualitatively. Tea polyphenol is not only an anti-tumor agent, but also a multidrug resistant modulator similar to quinidine. The multidrug resistance reversion mechanism of tea polyphenol seems to be its inhibition of the activity of P-glycoprotein. Tea polyphenol has the advantage of very low toxicity in tumor treatment

  18. Study of tea polyphenol as a reversal agent for carcinoma cell lines' multidrug resistance (study of TP as a MDR reversal agent)

    Energy Technology Data Exchange (ETDEWEB)

    Zhu Aizhi E-mail: zhuaizhi@263.net; Wang Xiangyun; Guo Zhenquan

    2001-08-01

    The aim of this study was to examine MDR1 expression product P-glycoprotein (Pgp) and study the effect and mechanism of tea polyphenol (TP) in reversion of multidrug resistance (MDR) in carcinoma cell lines. Immunocytochemical method was used for qualitative detection of Pgp. A comparative study of cytotoxicity and multidrug resistance reversion effect was made by MTT assay for tea polyphenol and quinidine in MCF-7 and MCF-7/Adr cell lines. The multidrug resistance reversion effect and mechanism were studied by measuring the uptake of {sup 99m}Tc-tetrofosmin in the carcinoma cell lines. (1) The Pgp overexpression in MCF-7/Adr cells was found to be strong positive, while the Pgp expression of MCF-7 was negative. (2) Although both tea polyphenol and quinidine could not remarkably change the toxicity of adriamycin to MCF-7, they could improve the sensitivity of MCF-7/Adr to adriamycin. The reversion index of tea polyphenol and quinidine was 3 and 10 respectively. (3) The cellular uptake of {sup 99m}Tc-tetrofosmin was remarkably lower in MCF-7/Adr than in MCF-7. The uptake of {sup 99m}Tc-tetrofosmin in MCF-7/Adr exhibited a 4, 13, 16 fold increase in the presence of 200, 400 and 500 {mu}g/ml of tea polyphenol respectively. The uptake of {sup 99m}Tc-tetrofosmin in MCF-7/Adr exhibited only a 4-fold increase in the presence of 200 {mu}M of quinidine. Immunocytochemistry can detect P-glycoprotein expression level qualitatively. Tea polyphenol is not only an anti-tumor agent, but also a multidrug resistant modulator similar to quinidine. The multidrug resistance reversion mechanism of tea polyphenol seems to be its inhibition of the activity of P-glycoprotein. Tea polyphenol has the advantage of very low toxicity in tumor treatment.

  19. Effect of black tea on antioxidant, textural, and sensory properties of Chinese steamed bread.

    Science.gov (United States)

    Zhu, Fan; Sakulnak, Ratchaneekorn; Wang, Sunan

    2016-03-01

    Black tea is rich in phenolic antioxidants and has various health benefits. Chinese steamed bread (CSB) is a traditional food produced through steaming of fermented dough. Black tea extracts were incorporated into northern style CSB formulation at varying concentrations up to 175mg gallic acid equivalent/gram of wheat flour. Gelatinization properties of wheat flour were not affected by the black tea addition. Rheological analysis of wheat flour showed that black tea increased pasting viscosity, consistency index of flow curves, and storage modulus (G') and loss modulus (G″) during dynamic oscillation. Black tea incorporation increased the antioxidant activity as measured by chemical assays, had little effect on textural properties, and increased the darkness of CSB. Sensory evaluation showed a good overall acceptance of black tea fortified CSB. PMID:26471674

  20. Green tea polyphenols and Tai Chi for bone health: Designing a placebo-controlled randomized trial

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    Chyu Ming-Chien

    2009-09-01

    Full Text Available Abstract Background Osteoporosis is a major health problem in postmenopausal women. Evidence suggests the importance of oxidative stress in bone metabolism and bone loss. Tea consumption may be beneficial to osteoporosis due to its antioxidant capability. However, lack of objective data characterizing tea consumption has hindered the precise evaluation of the association between tea ingestion and bone mineral density in previous questionnaire-based epidemiological studies. On the other hand, although published studies suggest that Tai Chi (TC exercise can benefit bone health and may reduce oxidative stress, all studies were conducted using a relatively healthy older population, instead of a high-risk one such as osteopenic postmenopausal women. Therefore, this study was designed to test an intervention including green tea polyphenol (GTP and TC exercise for feasibility, and to quantitatively assess their individual and interactive effects on postmenopausal women with osteopenia. Methods/Design One hundred and forty postmenopausal women with osteopenia (defined as bone mineral density T-score at the spine and/or hip between 1 to 2.5 SD below the reference database were randomly assigned to 4 treatment arms: (1 placebo group receiving 500 mg medicinal starch daily, (2 GTP group receiving 500 mg of GTP per day, (3 placebo+TC group receiving both placebo treatment and TC training (60-minute group exercise, 3 times per week, and (4 GTP+TC group receiving both GTP and TC training for 24 weeks. The outcome measures were bone formation biomarker (serum bone alkaline phosphatase, bone resorption biomarker (serum tartrate resistant acid phosphatase, and oxidative DNA damage biomarker (urinary 8-hydroxy-2'-deoxyguanosine. All outcome measures were determined at baseline, 4, 12, and 24 weeks. Urinary and serum GTP concentrations were also determined at baseline, 4, 12, and 24 weeks for bioavailability. Liver function was monitored monthly for safety. A

  1. Limitations of MTT and MTS-Based Assays for Measurement of Antiproliferative Activity of Green Tea Polyphenols

    OpenAIRE

    Wang, Piwen; Henning, Susanne M.; Heber, David

    2010-01-01

    Background The chemopreventive effect of green tea polyphenols, such as (-)-epigallocatechin-3-gallate (EGCG), has been well demonstrated in cell culture studies. However, a wide range of IC50 concentrations has been observed in published studies of the anti-proliferative activity of EGCG from different laboratories. Although the susceptibility to EGCG treatment is largely dependent on cancer cell type, the particular cell viability and proliferation assays utilized may significantly influenc...

  2. Limitations of MTT and MTS-Based Assays for Measurement of Antiproliferative Activity of Green Tea Polyphenols

    OpenAIRE

    Piwen Wang; Henning, Susanne M.; David Heber

    2010-01-01

    BACKGROUND: The chemopreventive effect of green tea polyphenols, such as (-)-epigallocatechin-3-gallate (EGCG), has been well demonstrated in cell culture studies. However, a wide range of IC(50) concentrations has been observed in published studies of the anti-proliferative activity of EGCG from different laboratories. Although the susceptibility to EGCG treatment is largely dependent on cancer cell type, the particular cell viability and proliferation assays utilized may significantly influ...

  3. Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy

    OpenAIRE

    Xianzhen Ma; Dadong Guo; Hongsheng Bi; Xiaofeng Xie; Junguo Guo; Yan Cui

    2014-01-01

    Purpose. The aim of this study was to investigate the effect of tea polyphenols (TP) ophthalmic gel on lens epithelial cells (LECs) in rabbits with silicone oil tamponade after vitrectomy. Methods. In this study, unilateral vitrectomy with silicone oil tamponade was performed using 2-month-old New Zealand white rabbits (n = 72); meanwhile, age-matched nonoperated rabbits (n = 18) were used as controls. The TP ophthalmic gel was administered topically in the surgical eyes till they were sacrif...

  4. Consumption of green tea, but not black tea or coffee, is associated with reduced risk of cognitive decline.

    Directory of Open Access Journals (Sweden)

    Moeko Noguchi-Shinohara

    Full Text Available Our objective was to determine whether the consumption of green tea, coffee, or black tea influences the incidence of dementia and mild cognitive impairment (MCI in older people. We conducted a population-based prospective study with Japanese residents aged >60 years from Nakajima, Japan (the Nakajima Project. Participants received an evaluation of cognitive function and blood tests. The consumption of green tea, coffee, and black tea was also evaluated at baseline. Of 723 participants with normal cognitive function at a baseline survey (2007-2008, 490 completed the follow up survey in 2011-2013. The incidence of dementia during the follow-up period (mean ± SD: 4.9 ± 0.9 years was 5.3%, and that of MCI was 13.1%. The multiple-adjusted odds ratio for the incidence of overall cognitive decline (dementia or MCI was 0.32 (95% CI: 0.16-0.64 among individuals who consumed green tea every day and 0.47 (95% CI: 0.25-0.86 among those who consumed green tea 1-6 days per week compared with individuals who did not consume green tea at all. The multiple-adjusted odds ratio for the incidence of dementia was 0.26 (95% CI: 0.06-1.06 among individuals who consumed green tea every day compared with those who did not consume green tea at all. No association was found between coffee or black tea consumption and the incidence of dementia or MCI. Our results indicate that green tea consumption is significantly associated with reduced risk of cognitive decline, even after adjustment for possible confounding factors.

  5. The Polyphenolic Composition of Cistus incanus Herbal Tea and Its Antibacterial and Anti-adherent Activity against Streptococcus mutans.

    Science.gov (United States)

    Wittpahl, Gesche; Kölling-Speer, Isabelle; Basche, Sabine; Herrmann, Eva; Hannig, Matthias; Speer, Karl; Hannig, Christian

    2015-12-01

    The Mediterranean plant Cistus incanus is rich in polyphenols and has shown several pharmacological activities, mainly antibacterial effects. Furthermore, in situ studies revealed that a C. incanus infusion reduces the initial bacterial adhesion in the oral cavity due to the polyphenols, an indication that C. incanus might reduce the risk of caries disease. In the present study, the polyphenols from four different commercial C. incanus herbal teas were extracted by standardized accelerated solvent extraction for in vitro tests and by an infusion for in situ tests. Both extracts were characterized qualitatively and quantitatively by high-performance liquid chromatography and only the polyphenol content differed slightly. By means of diode array detection and mass spectrometry, 29 polyphenols, including ellagitannins, flavanols, and glycosylated flavonols, were identified. Thereby, only quantitative but no qualitative differences between the four samples were detected. Furthermore, the in vitro antibacterial activity of the C. incanus accelerated solvent extracts against Streptococcus mutans, one of the primary cariogenic bacterial species, was examined using a live/dead assay (BacLight®). With this approach, C. incanus yielded antibacterial properties. Additional in situ experiments indicated that rinses with a C. incanus infusion reduced the initial bacterial colonization of enamel samples exposed to oral fluids for over eight hours. Furthermore, it was shown by transmission electron microscopy that the application of a C. incanus infusion modifies the ultrastructure of the acquired enamel pellicle, yielding a more electron-dense morphology. It can be assumed that the polyphenols are responsible for the observed effects. PMID:26291656

  6. Antagonism of phenanthrene cytotoxicity for human embryo lung fibroblast cell line HFL-I by green tea polyphenols

    Energy Technology Data Exchange (ETDEWEB)

    Mei Xin [Department of Tea Science, Zhejiang University, Hangzhou 310029 (China); Key Laboratory of Horticultural Plant Growth Development and Biotechnology of Ministry of Agriculture, Zhejiang University, Hangzhou 310029 (China); Wu Yuanyuan; Mao Xiao [Department of Tea Science, Zhejiang University, Hangzhou 310029 (China); Tu Youying, E-mail: youytu@zju.edu.c [Department of Tea Science, Zhejiang University, Hangzhou 310029 (China)

    2011-01-15

    Polycyclic aromatic hydrocarbons (PAHs) have been detected in some commercial teas around the world and pose a threat to tea consumers. However, green tea polyphenols (GTP) possess remarkable antioxidant and anticancer effects. In this study, the potential of GTP to block the toxicity of the model PAH phenanthrene was examined in human embryo lung fibroblast cell line HFL-I. Both GTP and phenanthrene treatment individually caused dose-dependent inhibition of cell growth. A full factorial design experiment demonstrated that the interaction of phenanthrene and GTP significantly reduced growth inhibition. Using the median effect method showed that phenanthrene and GTP were antagonistic when the inhibitory levels were less than about 50%. Apoptosis and cell cycle detection suggested that only phenanthrene affected cell cycle significantly and caused cell death; GTP lowered the mortality of HFL-I cells exposed to phenanthrene; However, GTP did not affect modulation of the cell cycle by phenanthrene. - Green tea polyphenols antagonised cytotoxicity of a low-ring PAH phenanthrene.

  7. Antagonism of phenanthrene cytotoxicity for human embryo lung fibroblast cell line HFL-I by green tea polyphenols

    International Nuclear Information System (INIS)

    Polycyclic aromatic hydrocarbons (PAHs) have been detected in some commercial teas around the world and pose a threat to tea consumers. However, green tea polyphenols (GTP) possess remarkable antioxidant and anticancer effects. In this study, the potential of GTP to block the toxicity of the model PAH phenanthrene was examined in human embryo lung fibroblast cell line HFL-I. Both GTP and phenanthrene treatment individually caused dose-dependent inhibition of cell growth. A full factorial design experiment demonstrated that the interaction of phenanthrene and GTP significantly reduced growth inhibition. Using the median effect method showed that phenanthrene and GTP were antagonistic when the inhibitory levels were less than about 50%. Apoptosis and cell cycle detection suggested that only phenanthrene affected cell cycle significantly and caused cell death; GTP lowered the mortality of HFL-I cells exposed to phenanthrene; However, GTP did not affect modulation of the cell cycle by phenanthrene. - Green tea polyphenols antagonised cytotoxicity of a low-ring PAH phenanthrene.

  8. Flavor characteristics of seven grades of black tea produced in Turkey.

    Science.gov (United States)

    Alasalvar, Cesarettin; Topal, Bahar; Serpen, Arda; Bahar, Banu; Pelvan, Ebru; Gökmen, Vural

    2012-06-27

    Seven grades of black tea [high-quality black tea (grades 1-3) and low-quality black tea (grades 4-7)], processed by ÇAYKUR Tea Processing Plant (Rize, Turkey), were compared for their differences in descriptive sensory analysis (DSA), aroma-active compounds (volatile compounds), and taste-active compounds (sugar, organic acid, and free amino acid compositions). Ten flavor attributes such as 'after taste', 'astringency', 'bitter', 'caramel-like', 'floral/sweet', 'green/grassy', 'hay-like', 'malty', 'roasty', and 'seaweed' were identified. Intensities for a number of flavor attributes ('after taste', 'caramel-like', 'malty', and 'seaweed') were not significantly different (p > 0.05) among seven grades of black tea. A total of 57 compounds in seven grades of black tea (14 aldehydes, eight alcohols, eight ketones, two esters, four aromatic hydrocarbons, five aliphatic hydrocarbons, nine terpenes, two pyrazines, one furan, two acids, and two miscellaneous compounds) were tentatively identified. Of these, aldeyhdes comprised more than 50% to the total volatile compounds identified. In general, high-grade quality tea had more volatiles than low-grade quality tea. With respect to taste-active compounds, five sugars, six organic acids, and 18 free amino acids were positively identified in seven grades of black tea, of which fructose, tannic acid, and theanine predominated, respectively. Some variations (p tea. The present study suggests that a certain flavor attributes correlate well with taste- and aroma-active compounds. High- and low-quality black teas should not be distinguished solely on the basis of their DSA and taste- and aroma-active compounds. The combination of taste-active compounds together with aroma-active compounds renders combination effects that provide the characteristic flavor of each grade of black tea. PMID:22642545

  9. Relationships between black tea consumption and key health indicators in the world: an ecological study

    OpenAIRE

    Beresniak, Ariel; Duru, Gerard; Berger, Genevieve; Bremond-Gignac, Dominique

    2012-01-01

    Objectives The aim of this study was to investigate potential statistical relationships between black tea consumption and key health indicators in the world. The research question is: Does tea consumption is correlated with one or more epidemiological indicators? Design Ecological study using a systematic data-mining approach in which the unit of the analysis is a population of one country. Setting Six variables, black tea consumption data and prevalence data of respiratory diseases, infectio...

  10. Green Tea Polyphenols Control Dysregulated Glutamate Dehydrogenase in Transgenic Mice by Hijacking the ADP Activation Site

    Energy Technology Data Exchange (ETDEWEB)

    Li, Changhong; Li, Ming; Chen, Pan; Narayan, Srinivas; Matschinsky, Franz M.; Bennett, Michael J.; Stanley, Charles A.; Smith, Thomas J. (CH-PA); (UPENN); (Danforth)

    2012-05-09

    Glutamate dehydrogenase (GDH) catalyzes the oxidative deamination of L-glutamate and, in animals, is extensively regulated by a number of metabolites. Gain of function mutations in GDH that abrogate GTP inhibition cause the hyperinsulinism/hyperammonemia syndrome (HHS), resulting in increased pancreatic {beta}-cell responsiveness to leucine and susceptibility to hypoglycemia following high protein meals. We have previously shown that two of the polyphenols from green tea (epigallocatechin gallate (EGCG) and epicatechin gallate (ECG)) inhibit GDH in vitro and that EGCG blocks GDH-mediated insulin secretion in wild type rat islets. Using structural and site-directed mutagenesis studies, we demonstrate that ECG binds to the same site as the allosteric regulator, ADP. Perifusion assays using pancreatic islets from transgenic mice expressing a human HHS form of GDH demonstrate that the hyperresponse to glutamine caused by dysregulated GDH is blocked by the addition of EGCG. As observed in HHS patients, these transgenic mice are hypersensitive to amino acid feeding, and this is abrogated by oral administration of EGCG prior to challenge. Finally, the low basal blood glucose level in the HHS mouse model is improved upon chronic administration of EGCG. These results suggest that this common natural product or some derivative thereof may prove useful in controlling this genetic disorder. Of broader clinical implication is that other groups have shown that restriction of glutamine catabolism via these GDH inhibitors can be useful in treating various tumors. This HHS transgenic mouse model offers a highly useful means to test these agents in vivo.

  11. Green tea polyphenol EGCG suppresses osteosarcoma cell growth through upregulating miR-1.

    Science.gov (United States)

    Zhu, Kewei; Wang, Wanchun

    2016-04-01

    (-)-Epigallocatechin-3-gallate (EGCG), the most abundant and active polyphenol in green tea, has been demonstrated to have anticancer effects in a wide variety of human cancer. MicroRNAs (miRNAs) are a class of short noncoding RNAs and play important role in gene regulation and are critically involved in the pathogenesis and progression of human cancer. This study aims to investigate the effects of EGCG on osteosarcoma (OS) cells and elucidate the underlying mechanism. Cellular function assays revealed that EGCG inhibited cell proliferation, induced cell cycle arrest and promoted apoptosis of OS cells in vitro, and also inhibited the growth of transplanted tumors in vivo. By miRNA microarray and RT-qPCR analysis, miR-1 was found to be significantly upregulated in MG-63 and U-2OS treated by EGCG in dose- and time-dependent manners, and miR-1 downregulation by inhibitor mimics attenuated EGCG-induced inhibition on cell growth of OS cells. We also confirmed that miR-1 was also frequently decreased in clinical OS tumor tissues. Moreover, both EGCG and miR-1 mimic inhibited c-MET expression, and combination treatment with EGCG and c-MET inhibitor (crizotinib) had enhanced inhibitory effects on the growth of MG-63 and U-2OS cells. Taken together, these results suggest that EGCG has an anticancer effect on OS cells, at least partially, through regulating miR-1/c-MET interaction. PMID:26499783

  12. Antimicrobial Activities of Nisin, Tea Polyphenols, and Chitosan and their Combinations in Chilled Mutton.

    Science.gov (United States)

    He, Li; Zou, Likou; Yang, Qianru; Xia, Jinghua; Zhou, Kang; Zhu, Yuanting; Han, Xinfeng; Pu, Biao; Hu, Bin; Deng, Wenwen; Liu, Shuliang

    2016-06-01

    Antimicrobial activities of nisin, tea polyphenols (TPs), and chitosan, and their combinations were evaluated against both Gram-positive bacteria (GPB) and Gram-negative bacteria (GNB) by the agar dilution method. Results showed that the MIC of nisin was 2.44 to 1250 mg/L for GPB and reached 5000 mg/L for GNB. The MICs of TPs and chitosan were 313 to 625 mg/L and 469 mg/L for GNB, and 156 to 5000 mg/L and 234 to 938 mg/L for GPB, respectively. These results indicated that TPs and chitosan exhibited inhibitory effects against both GPB and GNB, whereas nisin inhibited the growth of GPB only. Based on the orthogonal test of their MICs, and evaluation of preservative effect and sensory attributes in chilled mutton, the optimum combination was chosen as 0.625, 0.313, and 3.752 g/L for nisin, TPs, and chitosan, respectively. By using the optimum treatment, the shelf life of chilled mutton was extended from 6 to 18 d at 4 °C in the preservative film packages. These results indicate that the combination of nisin, TPs, and chitosan could be used as preservatives to efficiently inhibit the growth of spoilage microorganisms and pathogens in meat, thus improving the safety and shelf life of chilled mutton. PMID:27096939

  13. Inhibition of quorum sensing, biofilm, and spoilage potential in Shewanella baltica by green tea polyphenols.

    Science.gov (United States)

    Zhu, Junli; Huang, Xuzheng; Zhang, Fang; Feng, Lifang; Li, Jianrong

    2015-12-01

    We investigated the quorum sensing (QS) system of Shewanella baltica and the anti-QS related activities of green tea polyphenols (TP) against spoilage bacteria in refrigerated large yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines (DKPs) cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) were detected in the culture extract of S. baltica XH2, however, no N-acylhomoserine lactones (AHLs) activity was observed. Green TP at sub-inhibitory concentrations interfered with AI-2 and DKPs activities of S. baltica without inhibiting cell growth and promoted degradation of AI-2. The green TP treatment inhibited biofilm development, exopolysaccharide production and swimming motility of S. baltica in a concentration- dependent manner. In addition, green TP decreased extracellular protease activities and trimethylamine production in S. baltica. A transcriptional analysis showed that green TP repressed the luxS and torA genes in S. baltica, which agreed with the observed reductions in QS activity and the spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched in green TP significantly inhibited AI-2 activity of S. baltica. These findings strongly suggest that green TP could be developed as a new QS inhibitor for seafood preservation to enhance shelf life. PMID:26626353

  14. Anticlastogenic, antigenotoxic and apoptotic activity of epigallocatechin gallate: a green tea polyphenol

    International Nuclear Information System (INIS)

    Modulation of events characteristic of carcinogenesis or of cancer cells is being emphasized as a rational strategy to control cancer. Green tea polyphenol epigallocatechin gallate (EGCG) has been shown to be highly active as a cancer chemopreventive agent. Certain cellular and molecular events relevant to carcinogenesis are also modified by EGCG. The present investigation was carried out to examine the effects of EGCG on the cytogenetic change and DNA damage induced by toxicant H2O2 and carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Chinese hamster V-79 cells in culture. Cytogenetic change as evident by the formation of micronuclei and DNA damage in the form of comet tail length during single cell gel electrophoresis was found to be significantly suppressed by EGCG in a dose dependent manner. Cells preincubated with EGCG were protected from subsequent damage by the genotoxic agents. Apoptosis, a highly organized physiological mechanism to eliminate injured or abnormal cells, is also implicated in multistage carcinogenesis. Initiated cells, cells at promotional stage or fully transformed cells can be eliminated through apoptosis. It was observed that EGCG suppressed growth and proliferation of K-562 cells derived from human chronic myelogenic leukemia. Morphological features of treated cells and characteristic DNA fragmentation revealed that the cytotoxicity was due to induction of apoptosis. This was mediated by activation of caspase 3 and caspase 8. Results show that EGCG not only protects normal cells against genotoxic hazard but also eliminate cancer cells through induction of apoptosis

  15. Assessment of Fluoride Levels in Different Brands of Black and Green Tea Consumed in Iran

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    F. Mojarad

    2013-01-01

    Full Text Available Introduction & Objective: Tea is one of the most commonly consumed drinks in the world. Tea is recognized as a source of fluoride whose intake may increase the risk of developing dental fluorosis, particularly if other sources of fluoride augment the intake. Since the amount of fluoride in different types of tea consumed in our country is unknown, the purpose of this study was to evaluate the fluoride level of 22 commercial brands of tea popular in Iran. Materials & Methods: This descriptive study was conducted to assess the fluoride content of black tea (10 brands, bagged black tea (9 brands, and green tea (3 brands. 2 g from three samples of each tea brand taken out randomly were added to 200 ml deionized water and boiled for 10 minutes. After the infusion temperature coming down to the room temperature, the infusion was filtered and its volume made up to 200 ml by adding deionized water. The fluoride levels were measured using ion-selective electrode, and reported as mg/lit. Data were analyzed by ANOVA and Tukey test. Results: The Fluoride content was found 1.51 mg/lit in black tea bag, 1.038 mg/lit in green tea and 0.869±0.360 mg/lit in black tea sticks. (P<0.05, However, there was no statistically sig-nificant difference of fluoride concentration between green tea and black tea sticks(P= 0.52. Conclusion: This study showed that fluoride content of some tea brands were so high that drinking a few cups daily may increase the risk of developing dental fluorosis. Therefore, their consumption must be limited particularly in children, and in all inhabitants of regions with high fluoride levels in water supply.(Sci J Hamadan Univ Med Sci 2013; 19 (4:36-42

  16. Non-destructive determination of total polyphenols content and classification of storage periods of Iron Buddha tea using multispectral imaging system.

    Science.gov (United States)

    Xiong, Chuanwu; Liu, Changhong; Pan, Wenjuan; Ma, Fei; Xiong, Can; Qi, Li; Chen, Feng; Lu, Xuzhong; Yang, Jianbo; Zheng, Lei

    2015-06-01

    Total polyphenols is a primary quality indicator in tea which is consumed worldwide. The feasibility of using near infrared reflectance (NIR) spectroscopy (800-2500nm) and multispectral imaging (MSI) system (405-970nm) for prediction of total polyphenols contents (TPC) of Iron Buddha tea was investigated in this study. The results revealed that the predictive model by MSI using partial least squares (PLS) analysis for tea leaves was considered to be the best in non-destructive and rapid determination of TPC. Besides, the ability of MSI to classify tea leaves based on storage period (year of 2004, 2007, 2011, 2012 and 2013) was tested and the classification accuracies of 95.0% and 97.5% were achieved using LS-SVM and BPNN models, respectively. These overall results suggested that MSI together with suitable analysis model is a promising technology for rapid and non-destructive determination of TPC and classification of storage periods in tea leaves. PMID:25624215

  17. Long-term preservation of chilled canine semen using vitamin C in combination with green tea polyphenol.

    Science.gov (United States)

    Wittayarat, Manita; Kimura, Taichi; Kodama, Risa; Namula, Zhao; Chatdarong, Kaywalee; Techakumphu, Mongkol; Sato, Yoko; Taniguchi, Masayasu; Otoi, Takeshige

    2012-01-01

    Vitamin C and green tea polyphenol are known to have antioxidant effects. The aim of this study was to evaluate the quality of canine semen after preservation with diluents containing vitamin C and polyphenol at 5 degree C for 4 weeks. In experiment 1, we investigated the effects of vitamin C combined with polyphenol supplementation on chilled semen quality. The addition of vitamin C (0.5 or 1 mM) with 0.75 mg per mL polyphenol to semen extender provided significantly higher percentages of sperm motility and viability during cold storage compared to unsupplemented semen. In experiment 2, we determined the optimal working concentration of vitamin C in the semen extender by comparison of a range of concentrations between 0.1 and 20 mM. Supplementation of 0.5 mM vitamin C plus polyphenol yielded the highest percentages of sperm motility and viability; however, there was no beneficial effect on the plasma membrane and acrosomal integrity of the spermatozoa. PMID:22987243

  18. Spatial variability of theaflavins and thearubigins fractions and their impact on black tea quality.

    Science.gov (United States)

    Bhuyan, Lakshi Prasad; Borah, Paban; Sabhapondit, Santanu; Gogoi, Ramen; Bhattacharyya, Pradip

    2015-12-01

    The spatial distribution of theaflavin and thearubigin fractions and their impact on black tea quality were investigated using multivariate and geostatistics techniques. Black tea samples were collected from tea gardens of six geographical regions of Assam and West Bengal, India. Total theaflavin (TF) and its four fractions of upper Assam, south bank and North Bank teas were higher than the other regions. Simple theaflavin showed highest significant correlation with tasters' quality. Low molecular weight thearubigins of south bank and North Bank were significantly higher than other regions. Total thearubigin (TR) and its fractions revealed significant positive correlation with tasters' organoleptic valuations. Tea tasters' parameters were significantly and positively correlated with each other. The semivariogram for quality parameters were best represented by gaussian models. The nugget/sill ratio indicated a strong/moderate spatial dependence of the studied parameters. Spatial variation of tea quality parameters may be used for quality assessment in the tea growing areas of India. PMID:26604370

  19. Concentrations and solubility of selected trace metals in leaf and bagged black teas commercialized in Poland

    Directory of Open Access Journals (Sweden)

    L. Polechońska

    2015-09-01

    Full Text Available The objective of this study was to determine the concentrations of heavy metals in bagged and leaf black teas of the same brand and evaluate the percentage transfer of metals to tea infusion to assess the consumer exposure. Ten leaf black teas and 10 bagged black teas of the same brand available in Poland were analyzed for Zn, Mn, Cd, Pb, Ni, Co, Cr, Al, and Fe concentrations both in dry material and their infusion. The bagged teas contained higher amounts of Pb, Mn, Fe, Ni, Al, and Cr compared with leaf teas of the same brand, whereas the infusions of bagged tea contained higher levels of Mn, Ni, Al, and Cr compared with leaf tea infusions. Generally, the most abundant trace metals in both types of tea were Al and Mn. There was a wide variation in percentage transfer of elements from the dry tea materials to the infusions. The solubility of Ni and Mn was the highest, whereas Fe was insoluble and only a small portion of this metal content may leach into infusion. With respect to the acceptable daily intake of metals, the infusions of both bagged and leaf teas analyzed were found to be safe for human consumption.

  20. Bioavailability and activity of phytosome complexes from botanical polyphenols: the silymarin, curcumin, green tea, and grape seed extracts.

    Science.gov (United States)

    Kidd, Parris M

    2009-09-01

    Plant-derived polyphenols are increasingly receiving attention as dietary supplements for the homeostatic management of inflammation, to support detoxication, and for anticancer, weight loss, and other benefits. Their pro-homeostatic effects on genes, transcription factors, enzymes, and cell signaling pathways are being intensively explored, but the poor bioavailability of some polyphenols likely contributes to poor clinical trial outcomes. This review covers four polyphenol preparations with poor bioavailability and their complexation into phytosomes to bypass this problem. Silybin and the other silymarin flavonolignans from milk thistle conserve tissue glutathione, are liver-protective, and have anticancer potential. Curcumin and its related diphenolic curcuminoids have potent antioxidant, anti-inflammatory, and anti-carcinogenic properties. The green tea flavan-3-ol catechins have antioxidant, anti-inflammatory, cardio- and neuro-protective effects, and anti-carcinogenic benefits, with fat oxidation effects coupled to weight loss. The complex grape seed proanthocyanidin mix (including catechin and epicatechin monomers and oligomers) counters oxidative stress and protects the circulatory system. For each of these preparations, conversion into phytosomes has improved efficacy without compromising safety. The phytosome technology creates intermolecular bonding between individual polyphenol molecules and one or more molecules of the phospholipid, phosphatidylcholine (PC). Molecular imaging suggests that PC molecule(s) enwrap each polyphenol; upon oral intake the amphipathic PC molecules likely usher the polyphenol through the intestinal epithelial cell outer membrane, subsequently accessing the bloodstream. PC itself has proven clinical efficacy that contributes to phytosome in vivo actions. As a molecular delivery vehicle, phytosome technology substantially improves the clinical applicabilities of polyphenols and other poorly absorbed plant medicinals. PMID

  1. Fusion of Potentiometric & Voltammetric Electronic Tongue for Classification of Black Tea Taste based on Theaflavins (TF) Content

    Science.gov (United States)

    Bhattacharyya, Nabarun; Legin, Andrey; Papieva, Irina; Sarkar, Subrata; Kirsanov, Dmitry; Kartsova, Anna; Ghosh, Arunangshu; Bandyopadhyay, Rajib

    2011-09-01

    Black tea is an extensively consumed beverage worldwide with an expanding market. The final quality of black tea depends upon number of chemical compounds present in the tea. Out of these compounds, theaflavins (TF), which is responsible for astringency in black tea, plays an important role in determining the final taste of the finished black tea. The present paper reports our effort to correlate the theaflavins contents with the voltammetric and potentiometric electronic tongue (e-tongue) data. Noble metal-based electrode array has been used for collecting data though voltammetric electronic tongue where as liquid filled membrane based electrodes have been used for potentiometric electronic tongue. Black tea samples with tea taster score and biochemical results have been collected from Tea Research Association, Tocklai, India for the analysis purpose. In this paper, voltammetric and potentiometric e-tongue responses are combined to demonstrate improvement of cluster formation among tea samples with different ranges of TF values.

  2. Compositional, nutritional, and functional characteristics of instant teas produced from low- and high-quality black teas.

    Science.gov (United States)

    Alasalvar, Cesarettin; Pelvan, Ebru; Ozdemir, Kübra Sultan; Kocadağlı, Tolgahan; Mogol, Burçe Ataç; Paslı, Ayça Ayfer; Ozcan, Nihat; Ozçelik, Beraat; Gökmen, Vural

    2013-08-01

    Two types of instant teas produced from low- and high-quality black teas were examined for their proximate composition, dietary fiber, minerals, water-soluble vitamins, total phenolic content, various antioxidant assays, phenolics (flavanols, condensed phenolics, and phenolic acids), alkaloids, and carotenoids as well as taste-active compounds (sugars, organic acids, and free amino acids). Some variations, albeit to different extents, were observed (p fiber and fat were also present in both instant teas. Thirteen minerals, four water-soluble vitamins, six flavanols, two alkaloids, three condensed phenolics, one phenolic acid, and one carotenoid were identified. Total phenolic content varied between 17.35 and 17.82 g of gallic acid equivalents (GAE)/100 g instant tea. With regard to antioxidant activities, three different assays such as oxygen radical absorbance capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), and cupric ion reducing antioxidant capacity (CUPRAC) were measured. No significant differences (p > 0.05) in total phenolic, ORAC, TEAC, and CUPRAC contents between low- and high-quality instant teas were observed. With regard to taste-active compounds, 3 sugars, 5 organic acids, and 18 free amino acids were positively identified, of which fructose, tannic acid, and theanine predominated, respectively. The present work suggests that despite some differences, instant teas produced from low- and high-quality black teas should not be distinguished on the basis of their compositional, nutritional, and functional characteristics as well as taste-active compounds. PMID:23837397

  3. Antibacterial Activity of Iranian Green and Black Tea on Streptococcus Mutans : An In Vitro Study

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    M. J. Kharazi Fard

    2011-06-01

    Full Text Available Objective: Dental caries is a common infectious disease.Streptococcus mutans is the prevalent decay microorganism. The anti Streptococcus mutans activity of non fermented and semi-fermented tea has been shown. The aim of this study was to determine the anti Streptococcus mutans activity of Iranian green and black tea (non fermented and fermented type.Materials and Methods: The study was experimental. The aerial parts of wild-growing Camellia sinensis were collected from Lahijan province. The methanolic extract of green and black tea were examined on Streptococcus mutans (ATCC3566. Five different concentrations (50mg/ml, 100mg/ml, 200mg/ml, 300mg/ml and 400 mg/ml of tea extracts were tested using the well assay method. The agar dilution method recommended by the NCCLS standards (National Committee for Clinical Laboratory Standards was used. Theminimum inhibitory concentration (MIC was determined as the lowest concentration of extract inhibiting visible growth of the organism on the agar media plate. Minimum bactericidal concentration (MBC was detected from MIC.Results: The Iranian green and black tea had an antibacterial effect on 100 to 400 mg/ml concentrations. The minimum inhibitory concentration of green and black tea was 150 and 50 mg/ml, respectively. The mean diameter of inhibition zone were 9.5 mm and 10.9 mm for methanolic extract of green and black tea, respectively.Conclusion: Both Iranian non fermented (green tea and fermented (black tea have anti Streptococcus mutans activity in vitro. The anti Streptococcus mutans activity of black tea appears on a lower concentration than green tea.

  4. Bioaccessibility of Polyphenols from Plant-Processing Byproducts of Black Carrot (Daucus carota L.).

    Science.gov (United States)

    Kamiloglu, Senem; Capanoglu, Esra; Bilen, Fatma Damla; Gonzales, Gerard Bryan; Grootaert, Charlotte; Van de Wiele, Tom; Van Camp, John

    2016-03-30

    Plant-processing byproducts of black carrot represent an important disposal problem for the industry; however, they are also promising sources of polyphenols, especially anthocyanins. The present study focused on the changes in polyphenols from black carrot, peel, and pomace during in vitro gastrointestinal digestion. Total phenolic content (TPC), total monomeric anthocyanin content (TMAC), and total antioxidant capacity (TAC) were determined using spectrophotometric methods, whereas identification and quantification of polyphenols were carried out using UPLC-ESI-MS(E) and HPLC-DAD, respectively. TPC, TMAC, and TAC significantly decreased (23-82%) as a result of in vitro gastrointestinal digestion. Nevertheless, the amount of pomace anthocyanins released at all stages of in vitro gastrointestinal digestion was higher than black carrot anthocyanins, suggesting that pomace may be a better source of bioaccessible anthocyanins. Overall, the current study highlighted black carrot byproducts as substantial sources of polyphenols, which may be used to enrich food products. PMID:26262673

  5. Novel tea polyphenol-modified calcium phosphate nanoparticle and its remineralization potential.

    Science.gov (United States)

    He, Libang; Deng, Dongmei; Zhou, Xuedong; Cheng, Lei; ten Cate, Jacob M; Li, Jiyao; Li, Xudong; Crielaard, Wim

    2015-11-01

    Tea polyphenols (TP) are not only potent antimicrobial and antioxidant agents but also effective modifiers in the formation of nanosized crystals. Since nano-hydroxyapatite (n-HA) is known to enhance remineralization of dental hard tissue, our aims were to synthesize nanosized calcium phosphate particles incorporating TP and to test their potential as caries preventive agent. An ammonia water diffusion method was used to synthesize nanosized calcium phosphate particles (TP-CaP) in the presence of various amounts of TP. The resultant products were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The remineralization potential of the nano TP-CaP was then investigated in a 12-day pH-cycling model. Nano TP-CaP slurries, at pH 7.0 and pH 5.5, were applied onto preformed enamel lesions 4 × 3 min per day. n-HA slurries at pH 7.0 and pH 5.5 were used as positive controls, and deionized water was served as a negative control. SEM showed nanosized particles were only formed at 27 mg/mL of TP. Further characterization of the nanosized particles revealed the components were amorphous calcium phosphate, HA, and TP. Both surface microhardness and transverse microradiography analyses showed that nano TP-CaP at pH 5.5, but not at pH 7.0, significantly enhanced remineralization, to the same extent as the n-HA controls. Furthermore, significantly higher amount of TP was found in the supernatant of TP-CaP at pH 5.5 than those at pH 7.0. Since TP can inhibit bacterial growth and enzyme activities, the novel nanosized TP-CaP particle, at low pH, is a potential dual-functional-remineralization and antibacteria-product. PMID:25470574

  6. Green tea polyphenols and sulfasalazine have parallel anti-inflammatory properties in colitis models

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    Helieh S Oz

    2013-06-01

    Full Text Available Background: There is no cure for autoimmune chronic inflammatory bowel disease (IBD. IBD patients commonly use complementary and alternative medications of which the safety, efficacy and interaction with standard-of-care therapies are not fully known. Thus the consequences can become life-threatening. Sulfasalazine commonly used in IBD, potentially has severe adverse effects, including infertility, pulmonary fibrosis, lack of response and ultimately patients may require intestinal resection. We hypothesized that green tea polyphenols (GrTP, EGCG and sulfasalazine have similar anti-inflammatory properties. Methods: BALB/c mice received Dextran sodium sulfate (DSS to induce colitis (ulcerative colitis model. Exposure of IL-10 deficient mice (BALB/c-background to normal microbiota provoked enterocolitis (mimics Crohn’s disease. Animals were treated with agents incorporated into daily diets. Control animals received sham treatment. Results: DSS-treated animals developed severe bloody diarrhea and colitis (score 0-4, 3.2+0.27. IL-10 deficient mice developed severe enterocolitis as manifested by diarrhea, rectal prolapse and colonic lesions. Animals tolerated regimens (GrTP, EGCG, sulfasalazine with no major side effects, and further developed less severe colitis/enterocolitis. GrTP, EGCG and sulfasalazine significantly ameliorated colonic damage and histological scores in treated animals in a similar manner (GrTP vs DSS p<0.05; EGCG, sulfasalazine vs DSS p<0.01. The inflammatory markers TNFα (3-fold, IL-6 (14-fold and serum amyloid A (40-fold increased in colitic animals and significantly decreased with treatment regiments. In contrast, circulatory leptin levels decreased in colitic animals (2-fold. EGCG additionally reduced leptin levels (p<0.01 while GrTP and sulfasalazine had no effect on leptin levels (p<0.05. Hepatic and colonic antioxidants were significantly depleted in colitic animals and treatment regiments significantly restored

  7. Lysosomal trafficking of β-catenin induced by the tea polyphenol epigallocatechin-3-gallate

    International Nuclear Information System (INIS)

    β-Catenin is a cadherin-binding protein involved in cell-cell adhesion, which also functions as a transcriptional activator when complexed in the nucleus with members of the T-cell factor (TCF)/lymphoid enhancer factor (LEF) family of proteins. There is considerable interest in mechanisms that down-regulate β-catenin, since this provides an avenue for the prevention of colorectal and other cancers in which β-catenin is frequently over-expressed. We show here that physiologically relevant concentrations of the tea polyphenol epigallocatechin-3-gallate (EGCG) inhibited β-catenin/TCF-dependent reporter activity in human embryonic kidney 293 cells transfected with wild type or mutant β-catenins, and there was a corresponding decrease in β-catenin protein levels in the nuclear, cytosolic and membrane-associated fractions. However, β-catenin accumulated as punctate aggregates in response to EGCG treatment, including in human colon cancer cells over-expressing β-catenin endogenously. Confocal microscopy studies revealed that the aggregated β-catenin in HEK293 cells was extra-nuclear and co-localized with lysosomes, suggesting that EGCG activated a pathway involving lysosomal trafficking of β-catenin. Lysosomal inhibitors leupeptin and transepoxysuccinyl-L-leucylamido(4-guanido)butane produced an increase in β-catenin protein in total cell lysates, without a concomitant increase in β-catenin transcriptional activity. These data provide the first evidence that EGCG facilitates the trafficking of β-catenin into lysosomes, presumably as a mechanism for sequestering β-catenin and circumventing further nuclear transport and activation of β-catenin/TCF/LEF signaling

  8. The protective effects of tea polyphenols and schisandrin B on nephrotoxicity of mercury.

    Science.gov (United States)

    Liu, Wei; Xu, Zhaofa; Yang, Haibo; Deng, Yu; Xu, Bin; Wei, Yangang

    2011-12-01

    Mercury (Hg) is an occupational and environmental contaminant that is a well-recognized health hazard. To approach the concrete mechanisms of mercury nephrotoxicity and find out a new way to prevent it, the rats were subcutaneously injected with different dosages of mercuric chloride (HgCl(2))--0, 2.2, 4.4, and 8.8 μmol/kg. The levels of Hg, blood urea nitrogen (BUN), urine protein, glutathione (GSH), malondialdehyde (MDA) and activities of N-acetyl-beta-D-glucosaminidase (NAG), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were investigated, and the levels of reactive oxygen species (ROS) and apoptosis and the pathological changes were also observed. In addition, the effects of 1 mmol/kg tea polyphenols (TP) and 0.04 mmol/kg schisandrin B (Sch B) were studied at 8.8 μmol/kg HgCl(2). It was observed that the levels of Hg, BUN, urine protein, GSH, and MDA and activities of NAG, ALP, and LDH increased significantly; the activities of SOD and GSH-Px decreased significantly; the levels of ROS and apoptosis increased obviously; and many pathological changes occurred dose-dependently in the HgCl(2) injection groups. Further investigation indicated that pretreatment with TP and Sch B significantly reversed the toxic effects of HgCl(2). These results suggested that TP and Sch B might antagonize the nephrotoxicity caused by HgCl(2) exposure. PMID:21369715

  9. Tea polyphenols alleviate high fat and high glucose-induced endothelial hyperpermeability by attenuating ROS production via NADPH oxidase pathway

    OpenAIRE

    Zuo, Xuezhi; Tian, Chong; Zhao, Nana; Ren, Weiye; Meng, Yi; Jin, Xin; Zhang, Ying; Ding, Shibin; Ying, Chenjiang; Ye, Xiaolei

    2014-01-01

    Background Hyperglycemia-induced endothelial hyperpermeability is crucial to cardiovascular disorders and macro-vascular complications in diabetes mellitus. The objective of this study is to investigate the effects of green tea polyphenols (GTPs) on endothelial hyperpermeability and the role of nicotinamide adenine dinucleotide phosphate (NADPH) pathway. Methods Male Wistar rats fed on a high fat diet (HF) were treated with GTPs (0, 0.8, 1.6, 3.2 g/L in drinking water) for 26 weeks. Bovine ao...

  10. Comparative Analysis on Tea Polyphenols Extraction Rates of Four Kinds of Green Teas in Tongren%铜仁市四种绿茶茶多酚提取率的比较分析

    Institute of Scientific and Technical Information of China (English)

    鲁道旺; 鲁连芳; 罗磊

    2012-01-01

    To provide a scientific basis for tea quality and components in Tongren city, the experiment applied ferrous tartrate colorimetric method to compare the tea polyphenols extraction rates of four different green teas in Tongren city: Fanjing Mountain green tea from Yinjiang, ecological green tea from Songtao, Tai green tea from Shiqian, and the cloud green tea from Yanhe, which were picked in the same season and also in the same grade. The tea polyphenols extraction rates of Songtao ecological green teas from the same origin but in different picking seasons were also compared. The results showed that Tai green tea from Shiqian extracted the most, ecological green tea from Songtao, the cloud green tea from Yanhe, Fanjing Mountain green tea from Yinjiang progressively declinded. Meanwhile, among the Songtao ecological green teas in different picking seasons, the tea in Qingming season extracted the most, the tea before Qingming follows, then the tea in Guyu, the tea after Qingming the lest.%为对铜仁市茶叶品质成分提供科学依据,用酒石酸亚铁比色法对铜仁市的印江梵净山绿茶、松桃生态绿茶、石阡苔茶、沿河云雾绿茶四县同一季节同一等级茶叶中茶多酚提取率进行比较研究,同时对松桃生态绿茶同一产地不同采收时节茶叶中茶多酚的提取率进行比较研究。结果表明:茶多酚的提取率含量较高的是石阡苔茶,其次是松桃生态绿茶、沿河云雾绿茶、印江梵净山绿茶;松桃县云雾绿茶不同采收时节的茶叶中茶多酚提取率含量较高的是清明茶,其次是清明前茶、谷雨茶、清明后茶。

  11. A novel long-chain acyl-derivative of epigallocatechin-3-O-gallate prepared and purified from green tea polyphenols

    Institute of Scientific and Technical Information of China (English)

    陈平; 谭曜; 孙东; 郑小明

    2003-01-01

    Lipophilic tea polyphenols (LTP) were prepared by catalytic esterifieation of green tea polyphenols (GRIP) with hexadeeanoyl chloride. A novel long-chain aeyl-derivative of epigalloeateehin-3-o-gallate(EGCG) was first isolated from purification of LTP by high-speed eountereurrent ehromatography (HSCCC)using a solvent system composed of n-hexane-ethyl acetate-methanol-water ( 1 : 1 : 1 : 1, v/v) . The moleeularstructure of the acyl-derivative, Epigallocatechin-3-O-gallate-4'-O-hexadeeanate , was elucidated by meansof elemental analysis, IR, 1H-NMR and MS spectra.

  12. THE INFLUENCE OF TIME AND TYPE OF SOLVENT ON EFFICIENCY OF THE EXTRACTION OF POLYPHENOLS FROM GREEN TEA AND ANTIOXIDANT PROPERTIES OBTAINED EXTRACTS

    OpenAIRE

    Beata Drużyńska; Agnieszka Stępniewska; Rafał Wołosiak

    2007-01-01

    The aim of the study was to investigate the influence of the type of solvent and time on efficiency of the extraction of polyphenols and antioxidant properties extracts obtained from green tea. Extraction was conducted at room temperature using four solvents: water and 80% ethanol, 80% methanol and 80% acetone (water solutions, v/v) at 15, 30 and 60 minutes. Extracts were analysed for contents of polyphenols and catechins. The antioxidant properties have been determined by two methods: scaven...

  13. Evaluation of free radical scavenging activity of tea infusion of commercial tea products available in uae

    International Nuclear Information System (INIS)

    In the present study, twenty four commercial tea samples were assayed to determine their free radical scavenging activity and polyphenolic contents based on the brewing/infusing period. Tea samples were infused/brewed in 200 mL boiled water at 120 degree C for 1, 2 and 5 min, respectively. The radical scavenging activities of tea infusion/brewing were measured using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay method. The results were ranged from 67.81-90.51% for black tea bags, 90.37-94.51% for green tea bags, 24.66-92.25% for black tea powder, 16.08-93.06% for green tea powder and 32.90- 45.54% for Camomile herbal infusion. The results showed that 1 or 2 min black tea bags infusion exhibited highest radical scavenging activity than 5 min infusion. Antioxidant activities of tea powders were variable with the amount of tea powder. It was observed that antioxidant activity increased with increasing boiling time for smaller amount of sample. In contrary, shorter boiling time was better for larger amount of sample. The polyphenol contents of tea infusion were determined and the results were expressed as milligram quercetin equivalent/200 mL of tea infusion. The polyphenol content was increased with increased brewing period. In contrary, brewing for longer time rendered extract less antiradical activity. This study suggests that infusing tea bag for 1 or 2 min is sufficient for getting infusion with maximum radical scavenging activity and in case of tea powder, shorter boiling time is better for larger amount of powder or small amount of powder should be boiled for minimum 5 min for rendering extract with maximum radical scavenging activity. (author)

  14. Temperature and Time of Steeping Affect the Antioxidant Properties of White, Green, and Black Tea Infusions.

    Science.gov (United States)

    Hajiaghaalipour, Fatemeh; Sanusi, Junedah; Kanthimathi, M S

    2016-01-01

    Tea (Camellia sinensis) is the most highly consumed beverage in the world next to water. The common way of preparation is steeping in hot water which is varying for different type of tea. We investigated the antioxidant properties of 6 type of tea leaves under different time and temperatures of extraction method used. In general, all samples tested in this study demonstrated high levels of antioxidant capacity and antioxidant activity. The results indicate that the antioxidants activity is significantly affected by time and temperature of steeping and the highest was depending on the variety. White state values, green and black teas showed different levels of antioxidants under different extraction conditions. Overall, the highest activity for white tea was in prolonged hot and in some assays prolonged hot and cold extracts, whereas for green tea the highest activity observed in prolonged cold steeping while, for black tea was in short hot water infusion. The results of this study showed the antioxidant capacity of white and green tea was greater than black tea. PMID:26613545

  15. Acid production and conversion of konjac glucomannan during in vitro colonic fermentation affected by exogenous microorganisms and tea polyphenols.

    Science.gov (United States)

    Zhao, Xin-Huai; Geng, Qian

    2016-05-01

    Impacts of exogenous microorganisms and tea polyphenols on acid production and conversion during in vitro colonic fermentation of konjac glucomannan (KGM) were assessed in this study. Colonic fermentation of KGM by the fecal extract of healthy adults resulted in a propionate-rich profile, as acetic, propionic, butyric and lactic acids production were 16.1, 13.0, 3.3 and 20.2 mmol/L, respectively. Inoculation of one of ten exogenous microorganisms in the fermentative systems increased acetic, propionic and butyric acids production by 50-230%, 9-190% and 110-350%, respectively, and also accelerated lactic acid conversion by 14-40%. Tea polyphenols in the fermentative systems showed clear inhibition on both acid production and conversion; however, this inhibition could be partially or mostly antagonised by the inoculated exogenous microorganisms, resulting in improved acid production and conversion. In total, Lactobacillus brevis and Sterptococcus thermophilus were more able to increase acid production, and the propionate-rich profile was not changed in all cases. PMID:26902110

  16. Green tea polyphenols reduce body weight in rats by modulating obesity-related genes.

    Directory of Open Access Journals (Sweden)

    Chuanwen Lu

    Full Text Available Beneficial effects of green tea polyphenols (GTP against obesity have been reported, however, the mechanism of this protection is not clear. Therefore, the objective of this study was to identify GTP-targeted genes in obesity using the high-fat-diet-induced obese rat model. A total of three groups (n = 12/group of Sprague Dawley (SD female rats were tested, including the control group (rats fed with low-fat diet, the HF group (rats fed with high-fat diet, and the HF+GTP group (rats fed with high-fat diet and GTP in drinking water. The HF group increased body weight as compared to the control group. Supplementation of GTP in the drinking water in the HF+GTP group reduced body weight as compared to the HF group. RNA from liver samples was extracted for gene expression analysis. A total of eighty-four genes related to obesity were analyzed using PCR array. Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, including 3 orexigenic genes (Agrp, Ghrl, and Nr3c1; 7 anorectic genes (Apoa4, Cntf, Ghr, IL-1β, Ins1, Lepr, and Sort; and 2 genes that relate to energy expenditure (Adcyap1r1 and Adrb1. Intriguingly, the HF+GTP group restored the expression levels of these genes in the high-fat-induced obese rats. The protein expression levels of IL-1β and IL-6 in the serum samples from the control, HF, and HF+GTP groups confirmed the results of gene expression. Furthermore, the protein expression levels of superoxide dismutase-1 (SOD1 and catechol-O-methyltransferase (COMT also showed GTP-regulated protective changes in this obese rat model. Collectively, this study revealed the beneficial effects of GTP on body weight via regulating obesity-related genes, anti-inflammation, anti-oxidant capacity, and estrogen-related actions in high-fat-induced obese rats.

  17. Compositional variation among black tea across geographies and their potential influence on endothelial nitric oxide and antioxidant activity.

    Science.gov (United States)

    Dias, Paul Mark; Changarath, Jayashree; Damodaran, Anita; Joshi, Manoj Kumar

    2014-07-16

    Black tea (C. sinensis) consumption is well associated with enhanced endothelial function (EF) and reduced cardiovascular (CV) risk. This clinical end benefit is endorsed to flavonoids in tea. The black tea flavonoid composition varies across geographies and may impact its health benefits. Moreover, the underlying functional species and a precise working mechanism responsible for the observed health benefit also remain to be investigated. In this Article, we investigated the effect of black teas from various geographies (WoBTs) on different working mechanisms (antioxidant potential and endothelial function) proposed to influence certain risk factors of CVH, in vitro. Pearson correlation analysis showed that the antioxidant benefits are fairly influenced by majority of tea actives such as catechins, theaflavins, thearubigins, and phenolic acids, while NO potentiating effects are mainly regulated by catechins in black tea. The data also suggest that the net vascular function benefit of black tea is majorly influenced by NO enhancement, while mildly contributed by its antioxidant benefit. PMID:24990074

  18. An exposure and risk assessment for fluoride and trace metals in black tea

    Energy Technology Data Exchange (ETDEWEB)

    Sofuoglu, Sait C. [Izmir Institute of Technology, Department of Chemical Engineering and Environmental Research Center, Guelbahce, Urla 35430 Izmir (Turkey)], E-mail: cemilsofuoglu@iyte.edu.tr; Kavcar, Pinar [Izmir Institute of Technology, Department of Chemical Engineering and Environmental Research Center, Guelbahce, Urla 35430 Izmir (Turkey)], E-mail: pinarkavcar@iyte.edu.tr

    2008-10-30

    Exposure and associated health risks for fluoride and trace metals in black tea were estimated. Fifty participants were randomly recruited to supply samples from the tea that they drink, and self-administer a questionnaire that inquired about personal characteristics and daily tea intake. Analyzed trace metals included aluminum, arsenic, barium, cadmium, cobalt, chromium, copper, manganese, nickel, strontium, and zinc. Fluoride and four metals (Al, Cr, Mn, Ni) were detected in all samples while barium was detected only in one sample. The remaining metals were detected in >60% of the samples. Fluoride and aluminum levels in instant tea bag samples were greater than in loose tea samples (p < 0.05) while the differences in elemental concentrations of loose and pot bag tea samples were not significant. Median and 90th percentile daily tea intake rates were estimated as 0.35 and 1.1 l/day, respectively. Neither fluoride nor aluminum levels in black tea were found to associate with considerable risks of fluorosis and Alzheimer's disease, respectively. However, carcinogenic risk levels for arsenic were high; R > 1.0 x 10{sup -6} even at the median level. According to sensitivity analysis, daily tea intake was the most influencing variable to the risk except for arsenic for which the concentration distribution was of more importance.

  19. New ethanol extraction improves the anti-obesity effects of black tea.

    Science.gov (United States)

    Park, Bongju; Lee, Sangjin; Lee, Bonggyeong; Kim, Ingyum; Baek, Namjoon; Lee, Tae Ho; Lee, Seok-Yong; Son, Miwon; Park, Hyunsung

    2016-03-01

    Black tea has been reported to have anti-obesity effects in both rodents and humans. Gallic acid, an active component of black tea, decomposes quickly into pyrogallol in high-temperature solutions. This study introduced a new, aqueous ethanol extraction of black tea, which resulted in extracts with higher concentrations of gallic acid than conventional black tea extracts prepared by hot-water extraction or hot-ethanol extraction. We confirmed that, compared with the hot-water extract of black tea, the cold-ethanol extract of black tea (CE-BTE) had greater effects on reducing body weight and body fat, improving fatty liver, regulating blood glucose, and reducing blood cholesterol in the high-fat diet-induced obese mouse model. Nonetheless, although CE-BTE significantly reduced fat content, it did not reduce peroxisome proliferator-activated receptor (PPARγ) protein in epididymal fat tissue of HFD mice. We also showed that CE-BTE did not inhibit the function of PPARγ protein to drive adipogenesis of mouse 3T3-L1 preadipocytes. Considering that PPARγ is a master transcription factor not only for adipocyte differentiation, but also for adipose tissue function, such as glucose and lipid metabolism and insulin sensitivity, these results suggest that CE-BTE reduced fat mass and body weight without dampening fat cell homeostasis and insulin sensitivity. PMID:26604105

  20. Green tea polyphenols improve cardiac muscle mRNA, and protein levels of signal pathways related to insulin and lipid metabolism and inflammation in insulin-resistant rats

    Science.gov (United States)

    Epidemiologic studies indicate that the consumption of green tea polyphenols (GTP) may reduce the risk of coronary artery disease. To explore the underlying mechanisms of action at the molecular level, we examined the effects of GTP on cardiac mRNA and protein levels of genes involved in insulin an...

  1. Green tea polyphenols benefits body composition and improves bone quality in long-term high-fat diet-induced obese rats

    Science.gov (United States)

    This study investigated the effects of green tea polyphenols (GTP) on body composition and 2 bone properties in obese female rats. Thirty-six 3-month-old SD female rats were fed either a 3 low-fat (LF) diet (n = 12) or a high-fat (HF) diet (n= 24) for 4 months. Animals in the LF diet 4 group continu...

  2. Green tea polyphenol epigallocatechin-3-gallate inhibits TLR4 signaling through the 67-kDa laminin receptor on lipopolysaccharide-stimulated dendritic cells

    Energy Technology Data Exchange (ETDEWEB)

    Byun, Eui-Baek [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Choi, Han-Gyu [Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747 (Korea, Republic of); Sung, Nak-Yun [Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185 (Korea, Republic of); Byun, Eui-Hong, E-mail: ehbyun80@gmail.com [Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 301-747 (Korea, Republic of)

    2012-10-05

    Highlights: Black-Right-Pointing-Pointer Expressions of CD80, CD86, and MHC class I/II were inhibited by EGCG via 67LR. Black-Right-Pointing-Pointer EGCG-treated DCs inhibited LPS-induced pro-inflammatory cytokines via 67LR. Black-Right-Pointing-Pointer EGCG-treated DCs inhibited MAPKs activation and NF-{kappa}B p65 translocation via 67LR. Black-Right-Pointing-Pointer EGCG elevated the expression of the Tollip protein through 67LR in DCs. -- Abstract: Epigallocatechin-3-gallate (EGCG), a major active polyphenol of green tea, has been shown to down-regulate inflammatory responses in dendritic cells (DCs); however, the underlying mechanism has not been understood. Recently, we identified the 67-kDa laminin receptor (67LR) as a cell-surface EGCG receptor. In this study, we showed the molecular basis for the down-regulation of toll-like receptor 4 (TLR4) signal transduction by EGCG in DCs. The expressions of CD80, CD86, and MHC class I and II, which are molecules essential for antigen presentation by DCs, were inhibited by EGCG via 67LR. In addition, EGCG-treated DCs inhibited lipopolysaccharide (LPS)-induced production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-{alpha}, interleukin [IL]-1{beta}, and IL-6) and activation of mitogen-activated protein kinases (MAPKs), e.g., extracellular signal-regulated kinase 1/2 (ERK1/2), p38, c-Jun N-terminal kinase (JNK), and nuclear factor {kappa}B (NF-{kappa}B) p65 translocation through 67LR. Interestingly, we also found that EGCG markedly elevated the expression of the Tollip protein, a negative regulator of TLR signaling, through 67LR. These novel findings provide new insight into the understanding of negative regulatory mechanisms of the TLR4 signaling pathway and consequent inflammatory responses that are implicated in the development and progression of many chronic diseases.

  3. Estimation of Theaflavins (TF) and Thearubigins (TR) Ratio in Black Tea Liquor Using Electronic Vision System

    Science.gov (United States)

    Akuli, Amitava; Pal, Abhra; Ghosh, Arunangshu; Bhattacharyya, Nabarun; Bandhopadhyya, Rajib; Tamuly, Pradip; Gogoi, Nagen

    2011-09-01

    Quality of black tea is generally assessed using organoleptic tests by professional tea tasters. They determine the quality of black tea based on its appearance (in dry condition and during liquor formation), aroma and taste. Variation in the above parameters is actually contributed by a number of chemical compounds like, Theaflavins (TF), Thearubigins (TR), Caffeine, Linalool, Geraniol etc. Among the above, TF and TR are the most important chemical compounds, which actually contribute to the formation of taste, colour and brightness in tea liquor. Estimation of TF and TR in black tea is generally done using a spectrophotometer instrument. But, the analysis technique undergoes a rigorous and time consuming effort for sample preparation; also the operation of costly spectrophotometer requires expert manpower. To overcome above problems an Electronic Vision System based on digital image processing technique has been developed. The system is faster, low cost, repeatable and can estimate the amount of TF and TR ratio for black tea liquor with accuracy. The data analysis is done using Principal Component Analysis (PCA), Multiple Linear Regression (MLR) and Multiple Discriminate Analysis (MDA). A correlation has been established between colour of tea liquor images and TF, TR ratio. This paper describes the newly developed E-Vision system, experimental methods, data analysis algorithms and finally, the performance of the E-Vision System as compared to the results of traditional spectrophotometer.

  4. Antidiarrhoeal activity of Sri Lankan Dust grade Black Tea (Camellia sinensis L. in mice

    Directory of Open Access Journals (Sweden)

    W D Ratnasooriya

    2009-01-01

    Full Text Available This study examined the antidiarrhoeal potential of Sri Lankan black tea (Camellia sinensis in mice using high grown unblend Dust grade No: 1 tea. Different concentrations of black tea brew (BTB [84 mg/ ml (equivalent to 1.5 cups, 167 mg/ ml (equivalent to 3 cups, 501 mg/ ml (equivalent to 9 cups or 1336 mg/ ml (equivalent to 24 cups], or a high concentration (equivalent to 9 cups of green tea brew (GTB of Chinese and Japanese types or reference drug, toperamide (10 mg/ kg were orally administered to different groups of mice (N = 9-12/group and were subjected to two antidiarrhoeal tests: normal defecation test and castor oil-induced diarrhoea test. The results show that BTB of Sri Lankan Dust grade tea dose-dependently and markedly decreased the number of faecal boluses produced in the normal defecation test and improved the severity of the diarroheal condition in the castor oil-induced diarrhoea test. However, the antidiarrhoeal effect of BTB was superior to Japanese type of GTB and inferior to loperamide. BTB also prolonged the gastrointestinal transit time, impaired intestinal fluid secretion, increased intestinal fluid absorption and reduced in vitro nitric oxide production. It is concluded that Sri Lankan black tea possesses marked antidiarrhoeal activity supporting the folkloric claim that Sri Lankan black tea is a good remedy for acute non specific diarrhoea.

  5. Study on Polyphenol Oxidase Activities of Tea Cultivars Grown in Sichuan%四川主栽茶树品种的多酚氧化酶活性

    Institute of Scientific and Technical Information of China (English)

    陈昌辉; 齐桂年; 汪艳霞

    2011-01-01

    为了实现控制PPO酶,提高四川红、绿茶加工品质,对四川主要产荼区具代表性的5个茶树栽培品种鲜叶的多酚氧化酶(PPO)进行粗提,测定并比较不同品种的酶活特性.结果表明,当底物邻苯二酚的量为0.3~1.2 mL时,各个品种的酶活力随浓度增加而升高,而超过1.2 mL时,酶活力下降;在25~45℃范围内,各品种的酶活力随温度升高而升高,但超过45℃酶活力下降;在50~90℃下水浴处理20min,各品种的酶活力丧失程度不同;各品种的酶活力的适宜pH相对偏碱;相同浓度的3种抑制剂对各品种的酶活力均有一定的抑制效果.%Crude polyphenol oxidase(PPO) was separately obtained from 5 tea cultivars grown in Sichuan and enzymatic properties were examined to enhance processing qualities of green tea and black tea grown in Sichuan. The results showed that PPO activities increased with the increase of catechol substrate volume when the volume of catechol substrate ranged in 0. 3 ~ 1. 2mL, and decreased when catechol substrate volume was greater than 1.2mL; PPO activities also increased with the increase of temperature when the temperature ranged in 25~45℃, and decreased when the temperature was greater than 45℃;The PPO activities of 5 tea cultivars decreased in varying degree under the condition of 50~90℃ hot water treatment for 20 minutes; PPO could act under alkaline conditions; All the five inhibitors showed some degree of inhibition to PPO activities.

  6. Synergistic Effects of Potentilla fruticosa L. Leaves Combined with Green Tea Polyphenols in a Variety of Oxidation Systems.

    Science.gov (United States)

    Liu, Zehua; Luo, Ziwen; Jia, Caixia; Wang, Dongmei; Li, Dengwu

    2016-05-01

    Potentilla fruticosa L. leaves are widely used as tea in China, with many commercial "Jinlaomei" teas available in markets. It has been confirmed to possess significant antioxidant activity than that of butylated hydroxytoluene. In this study, the synergistic effects of P. fruticosa leaves extracts (PFE) combined with green tea polyphenols (GTP) were studied to elucidate their use in combination and find specific combinations with least concentrations that enhance the antioxidant activity. Isobolographic analysis indicated that the combination of PFE and GTP demonstrated extensive synergism (22/28 of the tests showed significant synergy) with 3:1 (PFE:GTP) exhibiting the best synergistic effect. Chemical compositions and content of 7 phenolic compounds in PFE, GTP, and their mixtures were evaluated by reverse-phase-high-performance liquid chromatography. While chemical composition did not seem to change after the combination, as no new peaks appeared in the chromatogram, and no existing peaks disappeared. However, the content of (+)-catechin, (-)-epigallocatechin (EGC), and , (-)-epigallocatechin gallate (EGCG) changed. Besides, antioxidant interactions of extracts and compounds were evaluated, EGC with hyperoside exhibited the greatest synergistic effect and the combination of 3:1 exhibited the strongest synergism (DPPH γ = 0.86, ABTS γ = 1.12, FRAP γ = 1.16). Therefore, interaction of phytochemicals may be one reason for the synergistic effects in PFE + GTP, with EGC + hyperoside likely playing an important role. This report provides a theoretical basis for the concomitant use of P. fruticosa blended with GTP, which can be effectively used as a compounded tea, dietary supplements, and substituent of synthetic antioxidant. PMID:27061936

  7. The sphingosine kinase-1 survival pathway is a molecular target for the tumor-suppressive tea and wine polyphenols in prostate cancer.

    Science.gov (United States)

    Brizuela, Leyre; Dayon, Audrey; Doumerc, Nicolas; Ader, Isabelle; Golzio, Muriel; Izard, Jean-Claude; Hara, Yukihiko; Malavaud, Bernard; Cuvillier, Olivier

    2010-10-01

    The sphingosine kinase-1/sphingosine 1-phosphate (SphK1/S1P) pathway has been associated with cancer promotion and progression and resistance to treatments in a number of cancers, including prostate adenocarcinoma. Here we provide the first evidence that dietary agents, namely, epigallocatechin gallate (EGCg, IC(50)≈75 μM), resveratrol (IC(50)≈40 μM), or a mixture of polyphenols from green tea [polyphenon E (PPE), IC(50)≈70 μM] or grapevine extract (vineatrol, IC(50)≈30 μM), impede prostate cancer cell growth in vitro and in vivo by inhibiting the SphK1/S1P pathway. We establish that SphK1 is a downstream effector of the ERK/phospholipase D (PLD) pathway, which is inhibited by green tea and wine polyphenols. Enforced expression of SphK1 impaired the ability of green tea and wine polyphenols, as well as pharmacological inhibitors of PLD and ERK activities, to induce apoptosis in PC-3 and C4-2B cells. The therapeutic efficacy of these polyphenols on tumor growth and the SphK1/S1P pathway were confirmed in animals using a heterotopic PC-3 tumor in place model. PC-3/SphK1 cells implanted in animals developed larger tumors and resistance to treatment with polyphenols. Furthermore, using an orthotopic PC-3/GFP model, the chemopreventive effect of an EGCg or PPE diet was associated with SphK1 inhibition, a decrease in primary tumor volume, and occurrence and number of metastases. These results provide the first demonstration that the prosurvival, antiapoptotic SphK1/S1P pathway represents a target of dietary green tea and wine polyphenols in cancer. PMID:20522783

  8. ANTI-CARIOGENIC EFFECTS OF POLYPHENOL PLANT PRODUCTS-A REVIEW

    Directory of Open Access Journals (Sweden)

    Parashar Kshitiz

    2011-03-01

    Full Text Available Polyphenols occurring in Grape seeds, cocoa, coffee and tea can have a role in the prevention of cariogenic processes, due to their antibacterial action. Cocoa polyphenol pentamers significantly reduce biofilm formation and acid production by Streptococcus mutans and S. sanguinis. In the same way, trigonelline, caffeine and chlorogenic acid occurring in green and roasted coffee interfere with S. mutans adsorption to saliva-coated hydroxyapatite beads. Studies carried out on green oolong tea and black tea indicate that tea polyphenols exert an anti-caries effect via an anti- microbial mode-of-action, and galloyl esters of epicatechin, epigallocatechin and gallocatechin showed increasing antibacterial activities. The anti-cariogenic effects against streptococci showed by polyphenols from cocoa, coffee, and tea suggest further studies to a possible application of these beverages in the prevention of pathogenesis of dental caries.

  9. Production of nanoparticles under benign conditions using vitamins, sugars, glutathione and polyphenols from tea and winery

    Science.gov (United States)

    In continuation of our developmental program for sustainable pathways to nanomaterials, an account of greener preparation of nanoparticles utilizing naturally occurring reagents such as vitamins, sugars, tea extracts, and biodegradable polymers will be presented which reduces or ...

  10. Anti-cancer properties of green Tea Probed viaquantum mechanics calculations

    Directory of Open Access Journals (Sweden)

    Azin Chitsazan

    2015-03-01

    Full Text Available 10.13005/ojc/310147Tea, from the plant camellia sinensis, is consumed in different parts of the world as green, black or oolong tea. Among all of these, however, the most significant effects on human health have been observed with the consumption of green tea. Green tea contains polyphenols, which include flavanols, flavandiols, flavonoids, and phenolic acids. Most of the green tea polyphenols (GTPs are flavonols, commonly known as catechins. There are four kinds of catechins mainly find in green tea: epicatechin, epigallocatechin, epicatechin-3-gallate, and EGCG. Green tea catechins have demonstrated significant antioxidant, anticarcinogenic, anti-inflammatory, thermogenic, probiotic, and antimicrobial properties in numerous human, animal, and in vitro studies. In the present study, four type catechins of green tea were studied. For each catechin ab initio method was employed for calculations and related parameters were computed.

  11. The use of green tea polyphenols for treating residual albuminuria in diabetic nephropathy: A double-blind randomised clinical trial.

    Science.gov (United States)

    Borges, Cynthia M; Papadimitriou, Alexandros; Duarte, Diego A; Lopes de Faria, Jacqueline M; Lopes de Faria, José B

    2016-01-01

    Prior research has shown that in experimental diabetes mellitus, green tea reduces albuminuria by decreasing podocyte apoptosis through activation of the WNT pathway. We investigated the effect of green tea polyphenols (GTP) on residual albuminuria of diabetic subjects with nephropathy. We conducted a randomised, double-blind study in 42 diabetic subjects with a urinary albumin-creatinine ratio (UACR) >30 mg/g, despite administration of the maximum recommended dose of renin-angiotensin (RAS) inhibition. Patients were randomly assigned to two equal groups to receive either GTP (containing 800 mg of epigallocatechin gallate, 17 with type 2 diabetes and 4 with type 1 diabetes) or placebo (21 with type 2 diabetes) for 12 weeks. Treatment with GTP reduced UACR by 41%, while the placebo group saw a 2% increase in UACR (p = 0.019). Podocyte apoptosis (p = 0.001) and in vitro albumin permeability (p < 0.001) were higher in immortalized human podocytes exposed to plasma from diabetic subjects compared to podocytes treated with plasma from normal individuals. In conclusion, GTP administration reduces albuminuria in diabetic patients receiving the maximum recommended dose of RAS. Reduction in podocyte apoptosis by activation of the WNT pathway may have contributed to this effect. PMID:27320846

  12. Antibacterial Activity of Iranian Green and Black Tea on Streptococcus Mutans : An In Vitro Study

    OpenAIRE

    Kharazi Fard, M. J.; Niakan, M; N. Jalayer Naderi; S. Zardi

    2011-01-01

    Objective: Dental caries is a common infectious disease.Streptococcus mutans is the prevalent decay microorganism. The anti Streptococcus mutans activity of non fermented and semi-fermented tea has been shown. The aim of this study was to determine the anti Streptococcus mutans activity of Iranian green and black tea (non fermented and fermented type).Materials and Methods: The study was experimental. The aerial parts of wild-growing Camellia sinensis were collected from Lahijan province. The...

  13. Antibacterial Activity of Iranian Green and Black Tea on Streptococcus Mutans: An In Vitro Study

    OpenAIRE

    Naderi, N. Jalayer; Niakan, M; Kharazi Fard, M. J.; S. Zardi

    2011-01-01

    Objective: Dental caries is a common infectious disease. Streptococcus mutans is the pre-valent decay microorganism. The anti Streptococcus mutans activity of non fermented and semi-fermented tea has been shown. The aim of this study was to determine the anti Streptococcus mutans activity of Iranian green and black tea (non fermented and fermented type). Materials and Methods: The study was experimental. The aerial parts of wild-growing Camellia sinensis were collected from Lahijan province. ...

  14. Detection of Radiation-Induced Hydrocarbons in Green, Black and Oolong Teas

    International Nuclear Information System (INIS)

    Hydrocarbons induced by gamma-irradiation of green, black, and oolong teas were analyzed to determine whether the hydrocarbons can be used as markers for detecting post-irradiation of these teas. The samples were irradiated at 0, 2.5, 5, 7.5, and 10 kGy. Detection was attempted by extracting fat from the teas, separation of hydrocarbons with florisil column chromatography, and identification of hydrocarbons by gas chromatography-mass spectroscopy (GC-MS). Concentration of hydrocarbons increased with the irradiation dose

  15. THE INFLUENCE OF TIME AND TYPE OF SOLVENT ON EFFICIENCY OF THE EXTRACTION OF POLYPHENOLS FROM GREEN TEA AND ANTIOXIDANT PROPERTIES OBTAINED EXTRACTS

    Directory of Open Access Journals (Sweden)

    Beata Drużyńska

    2007-03-01

    Full Text Available The aim of the study was to investigate the influence of the type of solvent and time on efficiency of the extraction of polyphenols and antioxidant properties extracts obtained from green tea. Extraction was conducted at room temperature using four solvents: water and 80% ethanol, 80% methanol and 80% acetone (water solutions, v/v at 15, 30 and 60 minutes. Extracts were analysed for contents of polyphenols and catechins. The antioxidant properties have been determined by two methods: scavenging activity against DPPH· and the method with ABTS·+. The abilities of extracts to chelate iron ions (II have been investigated too. On the basis of findings it turned out that both type of solvent and time have a significant influence on extraction of polyphenols from green tea. The best solvent for the extraction of total polyphenols was 80% acetone, whereas for catechins was water. The increase of extraction of polyphenols with prolonged extraction time was observed. All extracts had antioxidant properties against DPPH· and ABTS·+ and abilities to chelate iron ions (II.

  16. Determination of fluoride in black, green and herbal teas by ionselective electrode using a standard-addition method

    Directory of Open Access Journals (Sweden)

    Mochammad Yuwono

    2005-06-01

    Full Text Available Tea leaves are very rich in fluoride, since tea plants take up fluoride from the soil and accumulate in its leaves. Some of this fluoride is released into the infusion, which is drunk as tea. Fluoride in tea could be beneficial for the prevention of dental caries, but it may result in excessive intake and lead to enamel fluorosis. The purpose of this work was to determine the fluoride levels in 12 different brands and types of tea by means of a computer-controlled ion-selective electrode potentiometry using a standard-addition method. It is a rapid method which showed good accuracy and precision. Fluoride contents of tea infusions after 5 min ranged from 0.95 to 4.73 mg/l for black teas; from 0.70 to 1.00 mg/l for green teas, and from 0.26 to 0.27 mg/l for herbal teas. It was concluded that black teas and green teas examined may be important contributors to the total daily fluoride intake. However, the ingestion of some black teas that were found to have high fluoride content by children at the age of risk to dental fluorosis should be avoided.

  17. Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice: Analysis with Differential Acridine Orange/Ethidium Bromide Staining

    Directory of Open Access Journals (Sweden)

    María del Carmen García-Rodríguez

    2013-01-01

    Full Text Available This study was conducted to investigate the modulating effects of green tea polyphenols on genotoxic damage and apoptotic activity induced by hexavalent chromium [Cr (VI] in CD-1 mice. Animals were divided into the following groups: (i injected with vehicle; (ii treated with green tea polyphenols (30 mg/kg via gavage; (iii injected with CrO3 (20 mg/kg intraperitoneally; (iv treated with green tea polyphenols in addition to CrO3. Genotoxic damage was evaluated by examining micronucleated polychromatic erythrocytes (MN-PCEs obtained from peripheral blood at 0, 24, 48, and 72 h after treatment. Induction of apoptosis and cell viability were assessed by differential acridine orange/ethidium bromide (AO/EB staining. Treatment of green tea polyphenols led to no significant changes in the MN-PCEs. However, CrO3 treatment significantly increased MN-PCEs at 24 and 48 h after injection. Green tea polyphenols treatment prior to CrO3 injection led to a decrease in MN-PCEs compared to the group treated with CrO3 only. The average of apoptotic cells was increased at 48 h after treatment compared to control mice, suggesting that apoptosis could contribute to eliminate the DNA damaged cells induced by Cr (VI. Our findings support the proposed protective effects of green tea polyphenols against the genotoxic damage induced by Cr (VI.

  18. The microbiota is essential for the generation of black tea theaflavins-derived metabolites.

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    Huadong Chen

    Full Text Available BACKGROUND: Theaflavins including theaflavin (TF, theaflavin-3-gallate (TF3G, theaflavin-3'-gallate (TF3'G, and theaflavin-3,3'-digallate (TFDG, are the most important bioactive polyphenols in black tea. Because of their poor systemic bioavailability, it is still unclear how these compounds can exert their biological functions. The objective of this study is to identify the microbial metabolites of theaflavins in mice and in humans. METHODS AND FINDINGS: In the present study, we gavaged specific pathogen free (SPF mice and germ free (GF mice with 200 mg/kg TFDG and identified TF, TF3G, TF3'G, and gallic acid as the major fecal metabolites of TFDG in SPF mice. These metabolites were absent in TFDG- gavaged GF mice. The microbial bioconversion of TFDG, TF3G, and TF3'G was also investigated in vitro using fecal slurries collected from three healthy human subjects. Our results indicate that TFDG is metabolized to TF, TF3G, TF3'G, gallic acid, and pyrogallol by human microbiota. Moreover, both TF3G and TF3'G are metabolized to TF, gallic acid, and pyrogallol by human microbiota. Importantly, we observed interindividual differences on the metabolism rate of gallic acid to pyrogallol among the three human subjects. In addition, we demonstrated that Lactobacillus plantarum 299v and Bacillus subtilis have the capacity to metabolize TFDG. CONCLUSIONS: The microbiota is important for the metabolism of theaflavins in both mice and humans. The in vivo functional impact of microbiota-generated theaflavins-derived metabolites is worthwhile of further study.

  19. Effect of black tea intake on blood cholesterol concentrations in individuals with mild hypercholesterolemia: A diet-controlled randomized trial

    Science.gov (United States)

    Habitual intake of black tea has predominantly been associated with relatively lower serum cholesterol concentrations in observational studies. However, clinical trials evaluating the potential effects of black tea on serum cholesterol have had inconsistent results. These mixed results could be expl...

  20. Biomimetic 'Green' Synthesis of Nanomaterials Using Antioxidants-Vitamins, Glutathione and Polyphenols from Tea and Wine

    Science.gov (United States)

    The presentation summarizes our recent activity in chemical synthesis of nanomaterials via benign biomimetic ‘greener’ alternatives,1 such as the use antioxidants present in a variety of natural products, and ubiquitous glutathione in aqueous media.2 Vitamins B1, B2, C, and tea ...

  1. Differential effects of green tea polyphenol in the γ-irradiation induced human leukemic and lymphoblastic cell damage

    International Nuclear Information System (INIS)

    The green tea polyphenol (GTPP) has been known to exert antioxidant activity as a radical scavenger as well as cancer preventive and cancer inhibition effect. The aim of this study was to identify whether GTPP not only potentiate the growth inhibition effect in γ-irradiated human cancer cell but also exert protection action for irradiated human normal cell. GTPP (80% catechin including >45% EGCG) added in the HL60, human leukemia, and NC37, human lymphoblast, before irradiation. After establishing the amount of GTPP and the dose of radiation, the cells were treated with the GTPP for 6 hours and irradiated with the determined doses. Viability when 10 μg/ml GTPP added before γ-irradiation with 1 Gy to NC37 cells was not different in comparison with control but it when was irradiated with 3 Gy significantly different (1 Gy;P=0.126, 3 Gy;P=0.010). 20 ug/ml GTPP did not show significant difference in both NC37 cells irradiated with 1 Gy and 3 Gy (1 Gy;P=0.946, 3 Gy;P=0.096). Viabilities were significantly decreased with concentration of additional GTPP in HL60 with 1 of 3 Gy ( 1 Gy; 69.0±1.7% vs 42.4±1.3%, 3 Gy; 66.9±3.9% vs 44.2±1.6%). In vitro study, we certified that when the cells were irradiated with dose below 3 Gy, GTPP provide not only anticancerous effect against cancer cells but also radioprotective effect in normal cells simultaneously. Theses results suggest the possibility that consumption of green tea could give the radioprotective effect and maximize the effect on internal radiation such as radioiodine therapy concomitantly

  2. Adsorption of Pb(II on Spent Leaves of Green and Black Tea

    Directory of Open Access Journals (Sweden)

    Antonio Zuorro

    2010-01-01

    Full Text Available Problem statement: In recent years much attention has been focused on the use of biomass residues as low-cost adsorbents for the removal of heavy metal ions from contaminated waters. Spent tea leaves, an abundantly available material that is currently disposed of as a solid waste, are potentially suitable for such applications. Approach: To provide some information on the adsorption properties of tea waste, we evaluated the removal efficiency of lead ions by spent leaves of green and black tea. Batch adsorption experiments were made at 25 and 40°C at initial lead-ion concentrations between 0.01 and 2 g L-1. Equilibrium data were analyzed by the Langmuir equation to evaluate the maximum adsorption capacity and the equilibrium constant. The adsorption characteristics of the two materials were also compared with those of coffee grounds, activated carbon and Fullers earth. Results: Experimental data showed that removal efficiencies up to 98-99% can be achieved when using spent tea leaves as lead adsorbent. The results were only marginally affected by the type of tea waste. At low lead loading, the adsorption equilibrium was well described by the Langmuir equation, with a maximum adsorption capacity of 83-130 mg g-1 and an equilibrium constant ranging from 0.112-0.245 L mg-1. A comparison with other adsorbents provided the following order for lead removal efficiency: Black tea, coffee grounds > green tea > Fullers earth > activated carbon. Conclusion: The results from this study indicate that using spent tea leaves as an adsorbent may be an efficient and economical means for removing lead and, presumably, other heavy metal ions from aqueous solutions.

  3. 绿茶多酚提高壳聚糖包装膜的抗氧化性能%Adding green tea polyphenols enhances antioxidant of chitosan film

    Institute of Scientific and Technical Information of China (English)

    彭勇; 李云飞※; 项凯翔

    2013-01-01

      为了开发新型的抗氧化活性包装材料,该文以壳聚糖/绿茶多酚构成的复合膜为研究对象,比较分析了甘油和聚乙二醇对壳聚糖膜物理、机械、抗氧化和结构特性的影响。结果表明:未加入绿茶多酚前,壳聚糖甘油膜有着较低的水蒸汽透过系数和膨胀程度,其水蒸汽透过系数为8.84×10-11g/(m·s·Pa)。然而,聚乙二醇膜的抗拉强度和穿透力均高于甘油形成的膜。X射线衍射分析(XRD)表明应用质量分数40%的增塑剂导致11.7°和17.9°出现2个结晶峰,分别对应壳聚糖的水合结晶和无水结晶。加入绿茶多酚后,壳聚糖复合膜的抗氧化能力显著提高,1,1-二苯基-2-苦基肼(DPPH)自由基清除能力在5 min内达到83.9%。加入绿茶多酚显著降低了膜溶液的表观黏度,增加了膜的厚度、颜色和阻水性。从2种增塑剂的对比效果看,绿茶多酚对于甘油膜的机械性能影响较大,其断裂伸长率从42.02%急剧下降到9.19%,并且使膜的结晶结构从水合状态转变为非晶状态。但绿茶多酚对聚乙二醇膜的水蒸汽透过系数、膨胀程度和颜色影响较大。壳聚糖复合膜性能的这种差异与增塑剂中所含羟基的数量以及与绿茶多酚的配比有关。研究结果为绿茶多酚复合包装膜在实际中的应用提供了理论依据。%  Green tea polyphenols, which are extracted from tea and contain catechin, flavones, anthocyan, and phenolic acid, have been generally demonstrated to be powerful antioxidants. The development of antioxidant active packaging materials by the incorporation of tea polyphenols into biocompatible polysaccharide film has aroused significant interest. In order to broaden the application of chitosan film and develop environment-friendly composite film with green tea polyphenols, the effects of green tea polyphenols and plasticizer such as glycerol and polyethylene glycol 200 on the

  4. Aspergillus acidus from Puerh tea and black tea does not produce ochratoxin A and fumonisin B-2

    DEFF Research Database (Denmark)

    Mogensen, Jesper Mølgaard; Varga, J.; Thrane, Ulf;

    2009-01-01

    Puerh tea is a unique Chinese fermented tea. Unlike other teas it is stored for a long period of time. Aspergillus niger is claimed to be the dominant microorganism in the Puerh tea manufacturing process and also to be common on tea in general. A. niger sensu stricto is known to produce the...

  5. Regular consumption of black tea increases circulating kynurenine concentrations: A randomized controlled trial

    Directory of Open Access Journals (Sweden)

    J.M. Gostner

    2015-06-01

    General significance: The influence of black tea consumption on biomarkers of immune system activation could relate to its general health benefits. Data suggests that the net effect strongly depends on the individual immune state, being stimulatory in healthy individuals, while acting more immune dampening in situations with an inflammatory background.

  6. Chemical composition, plant secondary metabolites, and minerals of green and black teas and the effect of different tea-to-water ratios during their extraction on the composition of their spent leaves as potential additives for ruminants.

    Science.gov (United States)

    Ramdani, Diky; Chaudhry, Abdul Shakoor; Seal, Chris J

    2013-05-22

    This study characterized the chemical composition of green and black teas as well as their spent tea leaves (STL) following boiling in water with different tea-to-water ratios. The green and black tea leaves had statistically similar (g/kg dry matter (DM), unless stated otherwise) DM (937 vs 942 g/kg sample), crude protein (240 vs 242), and ash (61.8 vs 61.4), but green tea had significantly higher (g/kg DM) total phenols (231 vs 151), total tannins (204 vs 133), condensed tannins (176 vs 101), and total saponins (276 vs 86.1) and lower neutral detergent fiber (254 vs 323) and acid detergent fiber (211 vs 309) than the black tea leaves. There was no significant difference between the green and black tea leaves for most mineral components except Mn, which was significantly higher in green tea leaves, and Na and Cu, which were significantly higher in black tea leaves. A higher tea-to-water ratio during extraction significantly reduced the loss of soluble compounds into water and hence yielded more nutrient-rich STL. On the basis of these analyses it appears that the green and black tea leaves alongside their STL have the potential for use as sources of protein, fiber, secondary metabolites, and minerals in ruminant diets. The presence of high levels of plant secondary metabolites in either tea leaves or their STL suggests that they may have potential for use as natural additives in ruminant diets. PMID:23621359

  7. Joint effects of citrus peel use and black tea intake on the risk of squamous cell carcinoma of the skin

    Directory of Open Access Journals (Sweden)

    Harris Robin B

    2001-08-01

    Full Text Available Abstract Background Differences in tea drinking habits and/or citrus peel use are likely to vary by populations and could contribute to the inconsistencies found between studies comparing their consumption and cancer risk. Methods A population-based case-control study was used to evaluate the relationships between citrus peel use and black tea intake and squamous cell carcinoma (SCC of the skin. Moreover, we assessed the independent and interactive effects of citrus peel and black tea in the development of SCC. Results Hot and iced teas were consumed by 30.7% and 51.8% of the subjects, respectively. Peel consumption was reported by 34.5% of subjects. Controls were more likely than were cases to report citrus peel use (odds ratio (OR = 0.67 and hot tea intake (OR = 0.79. After adjustment for hot and iced tea intake, the ORs associated with citrus peel use were 0.55 and 0.69, respectively, whereas the corresponding adjusted ORs for hot and iced tea intake after adjustment for citrus peel use were 0.87 and 1.22 respectively. Compared with those who did not consume hot black tea or citrus peel, the adjusted ORs associated with sole consumption of hot black tea or citrus peel were 0.60 and 0.30, respectively. Subjects who reported consumption of both hot black tea and citrus peel had a significant marked decrease (OR= 0.22; 95% CI = 0.10 – 0.51 risk of skin SCC. Conclusion These results indicate that both citrus peel use and strong (hot black tea have independent potential protective effects in relation to skin SCC.

  8. Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

    Institute of Scientific and Technical Information of China (English)

    Xia Chen; Chang-Kai Sun; Guo-Zhu Han; Jin-Yong Peng; Ying Li; Yan-Xia Liu; Yuan-Yuan Lv; Ke-Xin Liu; Qin Zhou; Hui-Jun Sun

    2009-01-01

    AIM: To investigate the hepatoprotective activity of tea polyphenols (TP) and its relation with cytochrome P450 (CYP450) expression in mice. METHODS: Hepatic CYP450 and CYPb5 levels were measured by UV-spectrophotometry in mice 2 d after intraperitoneal TP (25, 50 and 100 mg/kg per day). Then the mice were intragastricly pre-treated with TP (100, 200 and 400 mg/kg per day) for six days before paracetamol (1000 mg/kg) was given. Their acute mortality was compared with that of control mice. The mice were pre-treated with TP (100, 200, and 400 mg/kg per day) for five days before paracetamol (500 mg/kg) was given. Hepatic CYP2E1 and CYP1A2 protein and mRNA expression levels were evaluated by Western blotting, immunohistochemical staining and transcriptase-polymerase chain reaction. RESULTS: The hepatic CYP450 and CYPb5 levels in mice of TP-treated groups (100, 200 and 400 mg/kg per day) were decreased in a dose-dependent manner compared with those in the negative control mice. TP significantly attenuated the paracetamol-induced hepatic injury and dramatically reduced the mortality of paracetamol-treated mice. Furthermore, TP reduced CYP2E1 and CYP1A2 expression at both protein and mRNA levels in a dose-dependent manner. CONCLUSION: TP possess potential hepatoprotective properties and can suppress CYP450 expression.

  9. Protective effect of tea polyphenol ophthalmic gel on lens epithelial cells in rabbits with silicone oil tamponade after vitrectomy.

    Science.gov (United States)

    Ma, Xianzhen; Guo, Dadong; Bi, Hongsheng; Xie, Xiaofeng; Guo, Junguo; Cui, Yan

    2014-01-01

    Purpose. The aim of this study was to investigate the effect of tea polyphenols (TP) ophthalmic gel on lens epithelial cells (LECs) in rabbits with silicone oil tamponade after vitrectomy. Methods. In this study, unilateral vitrectomy with silicone oil tamponade was performed using 2-month-old New Zealand white rabbits (n = 72); meanwhile, age-matched nonoperated rabbits (n = 18) were used as controls. The TP ophthalmic gel was administered topically in the surgical eyes till they were sacrificed. On days 45 and 90 after operation, the levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), and apoptosis of LECs were analyzed, respectively. Meanwhile, caspase-3 mRNA and protein levels were also determined. Results. The results indicate that the levels of ROS and apoptosis were elevated for LECs in rabbits after operation, whereas ΔΨm was decreased. Caspase-3 was apparently increased at both mRNA and protein levels. Treatment of TP ophthalmic gel could reduce the generation of ROS, maintain ΔΨm, inhibit the overexpression of caspase-3, and thus decrease the apoptosis of LECs of rabbits after operation. Conclusions. TP ophthalmic gel can efficiently inhibit caspase-3 overexpression, reduce the apoptosis of LECs, and prevent LECs from damage. Our result provides a new approach to prevent the development of complicated cataract after vitrectomy. PMID:25505926

  10. Synthesis, characterization and biocompatibility of ``green'' synthesized silver nanoparticles using tea polyphenols

    Science.gov (United States)

    Moulton, Michael C.; Braydich-Stolle, Laura K.; NadagoudaPresent Address: Pegasus Technical Services, 46 E. Hollister Street, Cincinnati, 45219, Ohio, Usa., Mallikarjuna N.; Kunzelman, Samantha; Hussain, Saber M.; Varma, Rajender S.

    2010-05-01

    Since ancient times, people have taken advantage of the antimicrobial effects of colloidal silver particles. Aside from the medical prospects, silver nanoparticles are found in a wide range of commercially available consumer products ranging from cosmetics to household cleansers. Current synthetic methods for creating silver nanoparticles typically call for potentially hazardous chemicals, extreme heat, and produce environmentally dangerous byproducts. Therefore, it is essential that novel ``green'' synthesis of nanoparticles becomes a reality, and it is imperative to fully analyze the potential toxic effects of these nanoparticles. In this study, we have shown that by reducing silver nitrate in solutions of tea extract or epicatechin of varying concentrations, spherical silver nanoparticles were formed that had controllable size distributions depending on the concentration of tea extract or epicatechin in the samples. Our ultra-resolution microscopy demonstrated that the nanoparticles were in fact interacting with the keratinocytes. Furthermore, evaluation of mitochondrial function (MTS) to assess cell viability and membrane integrity (LDH) in human keratinocytes showed that the silver nanoparticles were nontoxic. These results demonstrated that these nanoparicles are potentially biocompatible and warrant further evaluation in other biological systems.

  11. Synthesis, characterization and biocompatibility of "green" synthesized silver nanoparticles using tea polyphenols.

    Science.gov (United States)

    Moulton, Michael C; Braydich-Stolle, Laura K; Nadagouda, Mallikarjuna N; Kunzelman, Samantha; Hussain, Saber M; Varma, Rajender S

    2010-05-01

    Since ancient times, people have taken advantage of the antimicrobial effects of colloidal silver particles. Aside from the medical prospects, silver nanoparticles are found in a wide range of commercially available consumer products ranging from cosmetics to household cleansers. Current synthetic methods for creating silver nanoparticles typically call for potentially hazardous chemicals, extreme heat, and produce environmentally dangerous byproducts. Therefore, it is essential that novel "green" synthesis of nanoparticles becomes a reality, and it is imperative to fully analyze the potential toxic effects of these nanoparticles. In this study, we have shown that by reducing silver nitrate in solutions of tea extract or epicatechin of varying concentrations, spherical silver nanoparticles were formed that had controllable size distributions depending on the concentration of tea extract or epicatechin in the samples. Our ultra-resolution microscopy demonstrated that the nanoparticles were in fact interacting with the keratinocytes. Furthermore, evaluation of mitochondrial function (MTS) to assess cell viability and membrane integrity (LDH) in human keratinocytes showed that the silver nanoparticles were nontoxic. These results demonstrated that these nanoparicles are potentially biocompatible and warrant further evaluation in other biological systems. PMID:20648322

  12. Black Tea Lowers Blood Pressure and Wave Reflections in Fasted and Postprandial Conditions in Hypertensive Patients: A Randomised Study

    Directory of Open Access Journals (Sweden)

    Davide Grassi

    2015-02-01

    Full Text Available Hypertension and arterial stiffening are independent predictors of cardiovascular mortality. Flavonoids may exert some vascular protection. We investigated the effects of black tea on blood pressure (BP and wave reflections before and after fat load in hypertensives. According to a randomized, double-blind, controlled, cross-over design, 19 patients were assigned to consume black tea (129 mg flavonoids or placebo twice a day for eight days (13 day wash-out period. Digital volume pulse and BP were measured before and 1, 2, 3 and 4 h after tea consumption. Measurements were performed in a fasted state and after a fat load. Compared to placebo, reflection index and stiffness index decreased after tea consumption (p < 0.0001. Fat challenge increased wave reflection, which was counteracted by tea consumption (p < 0.0001. Black tea decreased systolic and diastolic BP (−3.2 mmHg, p < 0.005 and −2.6 mmHg, p < 0.0001; respectively and prevented BP increase after a fat load (p < 0.0001. Black tea consumption lowers wave reflections and BP in the fasting state, and during the challenging haemodynamic conditions after a fat load in hypertensives. Considering lipemia-induced impairment of arterial function may occur frequently during the day, our findings suggest regular consumption of black tea may be relevant for cardiovascular protection.

  13. Differences in quality parameters between types of commercial tea from Argentina

    Directory of Open Access Journals (Sweden)

    Ana Eugenia Thea

    2012-10-01

    Full Text Available Normal 0 21 false false false ES X-NONE X-NONE To determine differences in quality parameters between different types of tea elaborated in Argentina, water extract, crude fibre, caffeine, total polyphenol content, antioxidant activity, sorption isotherms and colour parameters (L, a, b were determined. Fifty-one industrial samples of commercially available teas including green tea, semifermented tea, black tea and black tea sub-product (BTSP were used for this study.Water extract contents were significantly higher in black tea and green tea (40.3% and 40.7% w/w, respectively, while semifermented tea and BTSP showed higher levels of crude fibre (22.3% and 20.4% w/w, respectively. Caffeine contents of black teas (2.7% w/w were significantly higher than in the other types of tea. Green teas revealed the highest concentrations of phenolic compounds and the major antioxidant activities (14.9 g GAE/100g dm and 30.0 g AAE/100g dm, respectively. The results also highlighted significant differences (p<0.05 in colour values between the different types of tea.

  14. In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)

    OpenAIRE

    Wanigasekera Daya Ratnasooriya; Walimuni Prabhashini Kaushalya Mendis Abeysekera; Chatura Tissa Dayendra Ratnasooriya

    2014-01-01

    Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was u...

  15. The Effect of Black Tea on Blood Pressure: A Systematic Review with Meta-Analysis of Randomized Controlled Trials

    OpenAIRE

    Arno Greyling; Rouyanne T Ras; Peter L Zock; Mario Lorenz; Hopman, Maria T.; Thijssen, Dick H. J.; Richard Draijer

    2014-01-01

    OBJECTIVE: Epidemiological evidence has linked consumption of black tea, produced from Camellia sinensis, with a reduced risk of cardiovascular diseases. However, intervention studies on the effects of tea consumption on blood pressure (BP) have reported inconsistent results. Our objective was to conduct a systematic literature review with meta-analysis of controlled human intervention studies examining the effect of tea consumption on BP. METHODS: We systematically searched Medline, Biosis, ...

  16. The effect of black tea and caffeine on regional cerebral blood flow measured with arterial spin labeling

    OpenAIRE

    Vidyasagar, Rishma; Greyling, Arno; Draijer, Richard; Corfield, Douglas R; Parkes, Laura M.

    2013-01-01

    Black tea consumption has been shown to improve peripheral vascular function. Its effect on brain vasculature is unknown, though tea contains small amounts of caffeine, a psychoactive substance known to influence cerebral blood flow (CBF). We investigated the effects on CBF due to the intake of tea components in 20 healthy men in a double-blinded, randomized, placebo-controlled study. On separate days, subjects received a single dose of 184 mg caffeine (equivalent to one strong espresso coffe...

  17. Tea extracts antioxidative potential in emulsified lipid systems

    Directory of Open Access Journals (Sweden)

    Anna Gramza-Michałowska

    2008-09-01

    Full Text Available Tea leaves (Camelia sinensis L. extracts are source of polyphenols, i.e. antioxidant components. Research showed possible tea extracts use in food technology, influencing contained lipids stability improvement. The aim of the research was comparison of different teas extracts activity in emulsified lipid system. The present research examined different teas: white, green, yellow, oolong and black aqueous and ethanol extracts. To evaluate the most potent addition level different tea extracts concentrations were chosen. Linoleic acid oxidative stability was measured by linoleic acid conjugated dienes produc-tion monitoring. Emulsions with additives were incubated 19 hours at 37°C in darkness. Results showed different tea extracts antioxidant activity, dependent on its concentration in examined system. Highest antioxidant activity, comparable to BHT and rosemary ex-tract was found in lipid sample with addition of yellow tea ethanol extract.

  18. Antioxidant effects of green tea

    OpenAIRE

    Forester, Sarah C.; Lambert, Joshua D

    2011-01-01

    Consumption of green tea (Camellia sinensis) may provide protection against chronic diseases, including cancer. Green tea polyphenols are believed to be responsible for this cancer preventive effect, and the antioxidant activity of the green tea polyphenols has been implicated as a potential mechanism. This hypothesis has been difficult to study in vivo due to metabolism of these compounds and poor understanding of the redox environment in vivo. Green tea polyphenols can be direct antioxidant...

  19. 绿茶多酚提取纯化工艺及药理作用%Extraction and purification technologies of green tea polyphenols and its pharmacological actions

    Institute of Scientific and Technical Information of China (English)

    张思佳; 黄璐; 牟峰

    2013-01-01

    绿茶多酚是绿茶的主要有效成分,多年研究发现绿茶多酚具有抗氧化、抗癌、抗肥胖、抗紫外线、抗龋齿等药理作用,且对神经退行性疾病和心血管系统疾病有一定的防治作用.本文对绿茶多酚近年来的提取纯化工艺及药理作用进行阐述,为绿茶的进一步开发提供参考.%Green tea polyphenols,which are major chemical constituents from green tea,have great pharmacological actions,such as antioxidant,anticancer,anti-obesity,anti-UV,against dental caries,improving cardiovascular function and neurodegenerative diseases.This review focused on the advances on extraction,purification technologies and pharmacological actions of green tea polyphenols in recent years.

  20. The mycoflora of tea

    OpenAIRE

    Halina Halweg; Barbara Podsiadło

    2014-01-01

    Aspergillus niger was most often isolated from 3 various black lea species dust obtained from packer factory. 10 species of black tea packaged in Poland and 28 various species of black tea and one species of green tea packed abroad. Other fungi were seen lees frequently. It seems that A. niger spores alone or with tea dust may induce various respiratory disorders in tea packers. Serum antibodies to antigens of A. niger and tea extracts imply that both may be important etiological factors.

  1. 茉莉酸甲酯诱导茶鲜叶制成的红茶中香气成分变化%Aroma changes of black tea prepared from methyl jasmonate treated tea plants

    Institute of Scientific and Technical Information of China (English)

    Jiang SHI; Li WANG; Cheng-ying MA; Hai-peng LV; Zong-mao CHEN; Zhi LIN

    2014-01-01

    Methyl jasmonate (MeJA) was widely applied in promoting food quality. Aroma is one of the key indicators in judging the quality of tea. This study examined the effect of exogenous MeJA treatment on tea aroma. The aroma components in black tea prepared from MeJA-treated fresh tea leaves were extracted using headspace solid-phase microextraction (HS-SPME) and were analyzed using gas chromatography-mass spectrometry (GC-MS) and GC-olfactometry (GC-O). Forty-five volatile compounds were identified. The results revealed that the MeJA-treated black tea had higher levels of terpene alcohols and hexenyl esters than the untreated tea. Moreover, several newly components, including copaene, cubenol, and indole, were induced by the MeJA treatment. The activities of poly-phenol oxidase andβ-glucosidase in fresh tea leaves changed after the MeJA treatment. Quantitative real-time po-lymerase chain reaction (qRT-PCR) analysis indicated that the gene expression levels of polyphenol oxidase andβ-primeverosidase were upregulated by two and three folds, respectively, by the MeJA treatment (P  创新要点:首次将茉莉酸甲酯应用于诱导茶叶香气品质提高,初步验证了茶叶香气品质提高的本质原因:相关酶活性提高,基因表达上调。  研究方法:采用顶空固相微萃取法(HS-SPME)对红茶香气进行富集,气相色谱-质谱联用仪(GC-MS)进行解吸附分析,实时定量多聚酶链式反应(qRT-PCR)分析茶鲜叶中香气相关酶基因表达。  重要结论:茉莉酸甲酯诱导后的茶鲜叶中多酚氧化酶(PPO)活性上升,β-葡萄糖苷酶活性下降;PPO和β-樱草糖苷酶基因表达上调,β-葡萄糖苷酶基因表达下调。茉莉酸甲酯诱导后的茶鲜叶能明显提高由其制成的红茶香气品质,且萜烯醇类和萜烯类含量明显提高。

  2. Limitations of MTT and MTS-based assays for measurement of antiproliferative activity of green tea polyphenols.

    Directory of Open Access Journals (Sweden)

    Piwen Wang

    Full Text Available BACKGROUND: The chemopreventive effect of green tea polyphenols, such as (--epigallocatechin-3-gallate (EGCG, has been well demonstrated in cell culture studies. However, a wide range of IC(50 concentrations has been observed in published studies of the anti-proliferative activity of EGCG from different laboratories. Although the susceptibility to EGCG treatment is largely dependent on cancer cell type, the particular cell viability and proliferation assays utilized may significantly influence quantitative results reported in the literature. METHODOLOGY/PRINCIPAL FINDINGS: We compared five widely used methods to measure cell proliferation and viability after EGCG treatment using LNCaP prostate cancer cells and MCF-7 breast cancer cells. Both methods using dyes to quantify adenosine triphosphate (ATP and deoxynucleic acid (DNA showed accuracy in the measurement of viable cells when compared to trypan blue assay and results showed good linear correlation (r = 0.95. However, the use of MTT (3-(4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide and MTS (3-(4,5-dimethylthiazol-2-yl-5-(3-carboxymethoxyphenyl-2-(4-sulfophenyl-2H-tetrazolium as indicators of metabolically active mitochondria overestimated the number of viable cells by comparison with the ATP, DNA, or trypan blue determinations. As a result, the observed IC(50 concentration of EGCG was 2-fold higher using MTT and MTS compared to dyes quantifying ATP and DNA. In contrast, when cells were treated with apigenin MTT and MTS assays showed consistent results with ATP, DNA, or trypan blue assays. CONCLUSIONS/SIGNIFICANCE: These results demonstrate that MTT and MTS -based assays will provide an underestimation of the anti-proliferative effect of EGCG, and suggest the importance of careful evaluation of the method for in vitro assessment of cell viability and proliferation depending on the chemical nature of botanical supplements.

  3. Increased chemopreventive effect by combining arctigenin, green tea polyphenol and curcumin in prostate and breast cancer cells.

    Science.gov (United States)

    Wang, Piwen; Wang, Bin; Chung, Seyung; Wu, Yanyuan; Henning, Susanne M; Vadgama, Jaydutt V

    2014-08-01

    The low bioavailability of most flavonoids limits their application as anti-carcinogenic agents in humans. A novel approach of treatment with a mixture of bioactive compounds that share molecular anti-carcinogenic targets may enhance the effect on these targets at low concentrations of individual compound, thereby overcoming the limitations of reduced bioavailability. We therefore investigated whether a combination of three natural products arctigenin (Arc), a novel anti-inflammatory lignan from the seeds of Arctium lappa, green tea polyphenol (-)-epigallocatechin gallate (EGCG) and curcumin (Cur) increases the chemopreventive potency of individual compounds. LNCaP prostate cancer and MCF-7 breast cancer cells were treated with 2-4 mg/L (about 5-10μM) Cur, 1μM Arc and 40μM EGCG alone or in combination for 48h. In both cell lines treatment with the mixture of Cur, Arc and EGCG synergistically increased the antiproliferative effect. In LNCaP cells both Arc and EGCG increased the pro-apoptotic effect of Cur. Whereas in MCF-7 cells Arc increased the cell apoptosis of Cur while EGCG enhanced cell cycle arrest of Cur at G0/G1 phase. The strongest effects on cell cycle arrest and apoptosis were achieved by combining all three compounds in both cell lines. The combination treatment significantly increased the ratio of Bax to Bcl-2 proteins, decreased the activation of NFκB, PI3K/Akt and Stat3 pathways and cell migration compared to individual treatment. These results warrant in vivo studies to confirm the efficacy of this novel regimen by combining Arc and EGCG with Cur to enhance chemoprevention in both prostate and breast cancer. PMID:25243063

  4. Green tea polyphenol epigallocatechin-3-gallate suppresses melanoma growth by inhibiting inflammasome and IL-1β secretion

    International Nuclear Information System (INIS)

    Highlights: ► EGCG inhibits melanoma cell growth at physiological doses (0.1–1 μM). ► EGCG inhibits melanoma cell growth via inflammasomes and IL-1β suppression. ► Inflammasomes and IL-1β could be potential targets for future melanoma therapeutics. -- Abstract: Epigallocatechin-3-gallate (EGCG), the major polyphenolic component of green tea, has been demonstrated to possess anti-inflammatory, antioxidant, anti-mutagenic and anti-carcinogenic properties. The anti-melanoma effect of EGCG has been previously suggested, but no clear mechanism of action has been established. In this study, we demonstrated that EGCG inhibits melanoma cell growth at physiological doses (0.1–1 μM). In the search for mechanisms of EGCG-mediated melanoma cell suppression, we found that NF-κB was inhibited, and that reduced NF-κB activity was associated with decreased IL-1β secretion from melanoma cells. Since inflammasomes are involved in IL-1β secretion, we investigated whether IL-1β suppression was mediated by inflammasomes, and found that EGCG treatment led to downregulation of the inflammasome component, NLRP1, and reduced caspase-1 activation. Furthermore, silencing the expression of NLRP1 abolished EGCG-induced inhibition of tumor cell proliferation both in vitro and in vivo, suggesting a key role of inflammasomes in EGCG efficacy. This paper provides a novel mechanism for EGCG-induced melanoma inhibition: inflammasome downregulation → decreased IL-1β secretion → decreased NF-κB activities → decreased cell growth. In addition, it suggests inflammasomes and IL-1β could be potential targets for future melanoma therapeutics.

  5. Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes

    Directory of Open Access Journals (Sweden)

    Zhengguang Wang

    2012-12-01

    Full Text Available The present study examined the effects of green tea polyphenols (GTP, insulin-like growth factor-I (IGF-I and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa media supplemented with one of the following supplements: GTP (0, 10, 15 and 20 µM, IGF-I (0, 50, 100 and 150 ng/mL or glucose (0, 1.5, 5.6 and 20 mM for 24 h. The results showed that oocytes cultured in media supplemented with 15 µM GTP, 100 ng/mL IGF-I and 5.6 mM glucose, in separate experiments, have higher cleavage and blastocyst rates compared with oocytes cultured in media without or with other concentration of GTP, IGF-I and glucose. Then these three substances with the concentration above were added together into SOFaa media and constituted a modified medium (Modified SOFaa. The COC were cultured in control SOFaa media and modified SOFaa media, respectively. The results showed that modified SOFaa media increased the intracellular glutathione concentration of matured oocytes, blastocyst rates and total cell numbers and cell numbers of inner cell mass per blastocyst compared with the control. Supplementing of GTP, IGF-I and glucose synchronously to maturation media can increase the intracellular GSH concentration of oocytes after in vitro maturation, and improve the embryo development and blastocyst quality in bovine.

  6. Efficacy of Black Tea as a Negative Oral Contrast Agent for MR Cholangiopancreatography (MRCP

    Directory of Open Access Journals (Sweden)

    Amir Hossein Jalali

    2010-05-01

    Full Text Available Background/Objective: Evaluation of the use of black tea as negative oral contrast agent in MR cholangiopancreatography (MRCP."nPatients and Methods: Thirty-five patients (mean age, 50.3±19.2 years, who were referred for MRCP, entered in this study. MRCP was performed before, after 5 minutes and after 15 minutes following consumption of 300 ml of black tea. Depiction of the gall bladder, cystic duct, proximal and distal parts of the common bile duct (CBD, intra hepatic ducts, ampula of Vater, main pancreatic duct (MPD and signal loss of the stomach and three different segments of the duodenum were investigated according to VAS and Lickert scores."nResults: Regarding visibility of seven different anatomical parts of the pancreatobiliary tree (gall bladder, cystic duct, CBD, common hepatic duct, intrahepatic duct, ampula of Vater and MPD, the post procedure images were better visualized only in the distal part of CBD, ampula of vater and MPD both in Lickert and VAS scoring (all Ps≤0.001."nThere was no significant difference between the images 5 and 15 minutes after tea consumption. Regarding the obliteration of high signal in the stomach and three different parts of the duodenum, all post tea images of the mentioned parts showed significant disappearance of high signal in Lickert and VAS scoring systems (all Ps≤0.001. "nConclusion: Black tea is an affordable, cheap, available, safe, and efficient oral negative contrast agent for MRCP which reduces the signal intensity of fluids in the gastrointestinal tract and is also efficient for better depiction of MPD, distal part of CBD and ampula.

  7. Effect of theanine and polyphenols enriched fractions from decaffeinated tea dust on the formation of Maillard reaction products and sensory attributes of breads.

    Science.gov (United States)

    Culetu, Alina; Fernandez-Gomez, Beatriz; Ullate, Monica; del Castillo, Maria Dolores; Andlauer, Wilfried

    2016-04-15

    The antiglycoxidative properties of theanine (TEF) and polyphenols enriched fractions (PEF) prepared from tea dust were tested in a model system composed of bovine serum albumin (BSA) and methylglyoxal (MGO). PEF caused a decrease in available free amino groups of BSA in presence and absence of MGO, suggesting the simultaneous occurrence of glycoxidation reaction and phenols-protein interaction. The presence of PEF and TEF inhibited formation of fluorescent advanced glycation end-products (AGEs). Moreover, theanine (TB) and polyphenol-enriched bread (PB) were formulated. A significant increase in free amino groups was observed in TBs with a dose-response effect, while addition of PEF in bread produced a significant decrease (p<0.05). PEF efficiently reduced fluorescent AGE formation in breads compared with TEF. The results are in line with the simplified model systems. PEF used as food ingredient allows obtaining a tasty food possessing health promoting properties and lower content of potential harmful compounds (AGEs). PMID:26616919

  8. Influence of regular black tea consumption on tobacco associated DNA damage and HPV prevalence in human oral mucosa.

    Science.gov (United States)

    Pal, Debolina; Banerjee, Sarmistha; Indra, Dipanjana; Mandal, Shyamsundar; Dum, Anirudha; Bhowmik, Anup; Panda, Chinmay Kr; Das, Sukta

    2007-01-01

    Black tea is more widely consumed than green tea worldwide, particularly in India. Therefore, it is necessary to focus attention on black tea with respect to its health promoting and anti-cancer actions. In order to establish the concept that black tea is a potential candidate for cancer prevention, it is important to provide epidemiological evidence derived from investigations of human populations. In view of this, the objective of the present study was to determine the correlation between nature of black tea consumption and DNA damage in normal subjects with or without tobacco habit and oral cancer patients, taking the latter as positive controls. Much experimental evidence points to associations between tobacco habit and HPV 16 and HPV 18 (Human Papilloma virus) infection. But no studies have taken into account the possible confounding effect of black tea consumption on DNA damage along with HPV infection. A pilot study was therefore undertaken. Comet assay was used to evaluate the DNA damage among normal subjects including tobacco users (n = 86), non-tobacco users (n = 45) and Oral cancer patients (n = 37). Percentage of damaged cells was scored in the buccal squamous cells of all subjects mentioned above. HPV analysis was performed on 79 samples (including 37 oral cancer patients). The evaluation of various confounding factors like age, tenure of tobacco habit and tea habit showed significant associations with DNA damage. The observations strongly indicate that regular intake of black tea at least above four cups can reduce tobacco associated DNA damage among normal tobacco users. HPV prevalence was not seen to be associated with age, tenure of tobacco habit or the tea drinking habit. PMID:17696743

  9. Green Tea Polyphenols Reduced Fat Deposits in High Fat-Fed Rats via erk1/2-PPARγ-Adiponectin Pathway

    OpenAIRE

    Chong Tian; Xiaolei Ye; Rui Zhang; Jia Long; Weiye Ren; Shibin Ding; Dan Liao; Xin Jin; Hongmei Wu; Shunqin Xu; Chenjiang Ying

    2013-01-01

    OBJECTIVE: Hypoadiponectinemia contributes to the development of obesity and related disorders such as diabetes, hyperlipidemia, and cardiovascular diseases. In this study we investigated the effects of green tea polyphenols (GTPs) on adiponectin levels and fat deposits in high fat (HF) fed rats, the mechanism of signaling pathway was explored as well. METHODS AND RESULTS: Male Wistar rats were fed with high-fat diet. GTPs (0.8, 1.6, 3.2 g/L) were administered via drinking water. Serum adipon...

  10. Kinetic and Thermodynamic Studies of the Adsorption of Crystal Violet onto Used Black Tea Leaves

    OpenAIRE

    Mohammad Abul Hossain; Md T. al-Hassan

    2013-01-01

    This study presents the kinetic and thermodynamic investigation of the adsorption of crystal violet (CV) on used black tea leaves (UBTL) from aqueous solution to evaluate the feasibility of the process. The effects of concentration, solution pH and temperature on adsorption kinetics were carried out in batch process. Kinetic studies have shown that the adsorption data partially follow simple first order, second order and pseudo second order kinetic equations for different initial concentrati...

  11. The Design and Implementation of Black Tea Traceability System Based on UHF RFID

    OpenAIRE

    Ling, Caijin; Liu, Jinxin; Wu, Jiayao; Wang, Qiushuang; Liu, Shumei; Chen, Haiqiang; Zhao, Chaoyi

    2014-01-01

    In order to realize the whole process of consumer-producer bidirectional traceability and distinguish the true from the false, according to Yinghong 9, planting, processing and circulation situation, we introduce UHF RFID and Network information technology, to design and develop the UHF RFID black tea traceability system. The system adopts .NET technology, uses Microsoft Visual Studio 2010 + VB.NET, Microsoft SQL SERVER 2005, T-SQL language for development and design, and uses the network arc...

  12. Honey as a potentiator in the bioactivity of lemon flavoured black tea

    OpenAIRE

    Pereira, Carla; Silva, Alexandra; Barros, Lillian; Vilas-Boas, Miguel; Ferreira, Isabel C.F.R.

    2012-01-01

    Honey plays an important role in human health by combating damage caused by oxidizing agents, namely reducing the risk of heart disease, cancer, immune-system decline, cataracts, and inflammatory processes, among others. The antioxidant activity of honey has been extensively reported and attributed to the anti oxidants present that include both enzymatic and non-enzymatic substances (Ferreira et al., 2009). The same properties have been attributed to black tea (Camellia sinensis) and lemon (C...

  13. Improvement of green tea polyphenol with milk on skin with respect to antioxidation in healthy adults: a double-blind placebo-controlled randomized crossover clinical trial.

    Science.gov (United States)

    Chiu, Hui-Fang; Lin, Tung-Yi; Shen, You-Cheng; Venkatakrishnan, Kamesh; Wang, Chin-Kun

    2016-02-01

    Green tea polyphenols (GTP) have been widely tested for their effects on several metabolic syndromes and degenerative diseases such as cancer, cardiovascular diseases, and diabetes. The present study was formulated to assess the physiological efficacy of green tea polyphenol infused with milk (GTPM) on skin integrity in correlation with antioxidative status in healthy adults. Forty-four healthy voluntary subjects were recruited and assigned to two groups, who drank 240 ml of mineral water mixed with either an experimental (GTPM) or placebo package (2 packs per day) for the following 6 months. The experimental group then switched to the placebo package, and vice versa, for a further 6 months, with one month of washout period in between. During the initial, 3(rd), 6(th), 10(th), and 13(th) month anthropometric measurements were performed and fasting blood samples were withdrawn for various biochemical assays. Skin examination was performed at the initial, 6(th) and 13(th) month. No significant alterations were observed in any of the anthropometric measurements. Administration of GTPM significantly increased (p < 0.05) the antioxidant index and antioxidant enzyme activities when compared with the placebo group, whereas a concomitant decrease in the levels of lipid peroxidation were noted. Moreover, GTPM intake notably improved skin integrity and texture by markedly lowering (p < 0.05) skin wrinkles and roughness in elderly subjects. GTPM proved to be an effective antioxidant by lowering oxidative stress and thereby ameliorating skin texture and integrity. PMID:26686527

  14. The Choice of China Tea

    Institute of Scientific and Technical Information of China (English)

    YuLiejiang; LiuHuadong

    2004-01-01

    THERE are three broad categories of China tea: green,black and oolong.Green tea is unfermented, and grows best in Zhejiang. According to Traditional Chinese Medicine it relieves internal heat. Black tea is

  15. A Double-Blind, Randomized, Placebo-Controlled Study to Evaluate the Efficacy and Safety of STA-2 (Green Tea Polyphenols) in Patients with Chronic Stable Angina

    Science.gov (United States)

    Lee, Tsung-Ming; Charng, Min-Ji; Tseng, Chuen-Den; Lai, Ling-Ping

    2016-01-01

    Background Green tea intake has been shown to improve endurance capacity in animal studies, but whether it has a similar effect on humans remains unclear. A randomized, double-blinded, parallel-controlled study was conducted to evaluate the short-term effect of STA-2, a pharmaceutical preparation of green tea polyphenols, in patients with effort-induced angina and documented positive exercise tolerance test. Methods A total of 79 patients recruited from three medical centers were randomly assigned to receive 2 STA-2 250 mg capsules, each containing 100 mg green tea polyphenols, three times daily, or placebo for six weeks after two consecutive symptom-limited treadmill exercise tests to ascertain the reproducibility of exercise tolerance. Results There was no difference in total exercise tolerance time from baseline to Week 6 between two groups (p = 0.639). There were also no observed improvements in subgroup analyses stratified by age, gender, and BMI categories. However, a significant reduction in low-density lipoprotein levels was shown in patients in the STA-2 group (-8.99 ± 19.18 mg/dL) versus the placebo group (0.57 ± 19.77 mg/dL), p = 0.037, with greater benefits in patients not taking antihyperlipidemic drugs (STA-2: -9.10 ± 19.96 mg/dL vs. placebo: 4.42 ± 15.08 mg/dL, p = 0.037). Conclusions STA-2 treatment for 6 weeks did not increase exercise time as measured on a treadmill. However, this study also indicated that STA-2 treatment could have potential beneficial effects on LDL-cholesterol concentrations. PMID:27471357

  16. An overview on Tea

    Directory of Open Access Journals (Sweden)

    Ruchi Verma

    2013-09-01

    Full Text Available This review focuses on types of tea, its processing methods, bioactive compounds which are present in it, health benefits, toxic effects, interaction with drugs and herbs, formulations involving tea polyphenols. Tea has been found to possess various bioactive leads and need of the hour is to characterize these leads for better product development.

  17. Development of tea polyphenols brown rice sponge cake%茶多酚糙米海绵蛋糕的研制

    Institute of Scientific and Technical Information of China (English)

    徐红利; 吴跃; 李安平; 张燕秋

    2015-01-01

    以糙米粉和蛋糕粉为主要原料,研究茶多酚糙米海绵蛋糕的配方。通过单因素和正交试验考察了蛋糕粉、茶多酚、白砂糖和牛奶添加量对茶多酚糙米海绵蛋糕感官品质和质构品质的影响,优化了茶多酚糙米海绵蛋糕的配方,确定了其最佳配方:蛋糕粉添加量20%,茶多酚添加量0.8%,白砂糖添加量80%,牛奶添加量40%,鸡蛋添加量180%,食用油添加量10%(以糙米粉与蛋糕粉的总量为基准)。按此配方所制得的糙米海绵蛋糕不含其他添加剂,表面呈金黄色,外形规范,隆起正常,而且营养价值高,具有焙烤米香风味,咀嚼性好,不易形成碎渣,爽口、有弹性,存放时间延长。%The formulaof tea polyphenols–brown rice spongecake was studied by using brown rice powder andcake powder as themain rawmaterials.The single factor andorthogonal test examined the effectsof the addition amountofcake powder,tea polyphenols,sugar andmilkon the sensory quality and structure qualityof tea polyphenols–brown rice spongecake.The formulaof tea polyphenols–brown rice spongecake wasoptimized and the best formula was 20%cake powder,0.8% tea polyphenols,80%sugar,40%milk,180% egg and 10%oil(basedon the total amountof brown rice powder andcake powder).Thecakemade withoutother additiveshad golden surface,appearance specification and normal uplift. In addition,ithadhigh nutritional value,baked rice fragrant flavor,goodchewiness,not easily broken slag formation,prolong storage time and was refreshing and elastic.

  18. Changes of Constituents and Activity to Apoptosis and Cell Cycle During Fermentation of Tea

    Directory of Open Access Journals (Sweden)

    Wei Shi

    2011-03-01

    Full Text Available Tea is believed to be beneficial for health, and the effects of the fermentation process on its contributions to apoptosis and cell cycle arrest of gastric cancer cells have not been completely investigated. In this study, the chemical components in green tea, black tea and pu-erh tea aqueous extracts were analyzed and compared. The polysaccharide and caffeine levels were substantially higher in the fermented black tea and pu-erh tea, while the polyphenol level was higher in the unfermented green tea. Hence, a treatment of tea aqueous extract and the components, which are emerging as promising anticancer agents, were pursued to determine whether this treatment could lead to enhance apoptosis and cell cycle arrest. In the human gastric cancer cell line SGC-7901, the cell viability and flow cytometry analysis for apoptotic cells indicated effects in a dose-dependent inhibition manner for the three tea treatment groups. The apoptosis rates were found to be elevated after 48 h of treatment with 31.2, 125, and 500 μg/mL of green tea extract, the higher catechins content may be involved in the mechanism. Cell cycle was arrested in S phase in the fermented black tea and pu-erh tea, and the populations were significantly decreased in G2/M phases, possibly due to the oxidation of tea polyphenols, which causes an increase of theabrownins. CCC-HEL-1 normal cells were not sensitive to tea extract. These findings suggest that the fermentation process causes changes of the compounds which might be involved in the changes of cell proliferation inhibition, apoptosis induction and cell cycle arrest.

  19. Kinetic evaluation of black tea color index and determination of factors, which influence its by UV/Vis spectrophotometry

    International Nuclear Information System (INIS)

    The tea is the most important product that is widely consumed in the world. Its color is one of the basic parameter. It is known that the best color of an infusion of black tea is a bright orange-red color. [1] Rates of color extraction and amount of its extract are also important in tea quality. Various methods have been reported for this purpose which are mainly time consumable. In this research, absorbance at 480 nm is used (instate of total spectrum) as a fast and convent color index. The experiments have shown that this proposed method have good results. Effects of various factors on the color of an infusion of black tea was investigated; including: leaf size, temperature, ph, total dissolved solid and water hardness. Finally, its kinetic diagrams were determined which give

  20. 论安化黑茶的绿色包装%Talk about the Green Packaging of Anhua Black Tea

    Institute of Scientific and Technical Information of China (English)

    庞野营

    2011-01-01

    通过对绿色包装的内涵及原则的论述,结合安化黑茶包装材料的发展现状,分析了绿色包装材料在安化黑茶包装设计中的重要性。在此基础上,进一步提出了安化黑茶包装材料基于地域资源、地域文化之上的绿色发展方向,提高了安化黑茶包装与社会、自然相互协调、共生互补的境界,确保绿色设计思想在黑茶包装中的可行性。%Through discussion of the connotation and principle of green packaging, combined with the development situation of Anhua black tea packaging materials, it analyzed the importance of green packaging materials in packaging design of Anhua black tea. Based on this, it further proposed the green development orientation of packaging materials of Anhua black tea based on region resources and culture. Finally, it enhanced the anhua black tea packaging to achieve sustainable development with the society and nature, which guaranteed the feasibility of green design ideas in the black tea packaging.

  1. Iranian Black Tea and Cowslip Extracts Induce Tumor Necrosis Factor-Alpha Secretion from Mouse Macrophage Cell Culture

    OpenAIRE

    Nadi, Mahmoud; Mosaffa, Nariman; Karimi, Forouzan; Mohammad KAMALINEJAD; Farrokhi, Babak; Anissian, Arash; Pakzad, Parviz

    2010-01-01

    Many species of tea (Camellia sinensis) and cowslip (Echium amoenum) are used in Iranian traditional medicine. The aim of this study was to conduct the survey on the ability of Iranian black tea and cowslip extracts on secretion of tumor necrosis factor-alpha (TNF-alpha) by non-infected and infected mouse macrophages. A macrophage infection model with Legionella pneumophila and enzyme linked immunosorbent assay (ELISA) technique was used in this study. Research showed that the concentrations ...

  2. Studies of Anti-oxidation Activity of Tea Polyphenols%茶多酚抗氧化作用的研究

    Institute of Scientific and Technical Information of China (English)

    王媛; 徐志强; 殷红; 陈小波; 荣华; 廖国周; 谷大海; 樊月圆; 葛长荣; 贾俊静

    2013-01-01

    根据世界卫生组织报道,癌症、糖尿病、肥胖症、心血管系统疾病等是目前全球发病率较高,严重危害人类健康的疾病,已成为医学和生物制药领域攻克的目标和研究热点.而开发利用天然药物及天然抗氧化剂已成为21世纪生物医学、食品科学及现代农业的一个重要研究内容.茶多酚是目前尚不能人工合成的纯天然、多功能、高效能的抗氧化剂和自由基净化剂,具有抗氧化、抗癌、抗突变、降血脂、降血压等多种药用价值及保健功能.近年来茶多酚的保健功能研究一直是国内外研究的热点之一.以国内外研究结果为依据,阐述了茶多酚抗氧化作用机理、特性及国内外的发展情况,为充分发挥茶多酚在生产实践中的作用提供参考.%It has been reported that the cancer, diabetes, obesity, cardiovascular system diseases are significant public health problems with higher incidence disease according to the data from World Health Organization. Currently, a lot of researches have been focus on these diseases in medical and biological pharmaceutical field for development and utilization of natural antioxidant to restrain these diseases. Tea polyphe-nol is natural, multi-function, high efficiency antioxidants and free radical scavenger. It has play important roles in anti-oxidation, anti-tumor and mutation, reducing low-density lipoprotein-LDL and possesses many kinds of medical value and health care function. In recent years, huge researches have reported the tea polyphenol health care functions. Tea polyphenol anti-oxidation mechanism, characteristics and health benefits were elaborated to provide useful data for biopharmaceutical industry.

  3. Equilibrium adsorption of rhodamine B on used black tea leaves from acidic aqueous solution

    OpenAIRE

    Mohammad Abul Hossain; M. Atiqur Rahman

    2012-01-01

    The presence of carcinogenic dye like rhodamine B (Rh-B) in textile wastewater affects the quality of water to consumers. The adsorption of Rh-B on used black tea leaves (UBTL) was studied in batch process to investigate its removal efficiency. The effects of contact time, concentration, temperature, pH etc. on adsorption have been investigated. The UV-visible spectrophotometer was used for analysis of Rh-B at constant pH. The adsorption isotherms were constructed for differ...

  4. Investigation of free amino acid, total phenolics, antioxidant activity and purine alkaloids to assess the health properties of non-Camellia tea

    Directory of Open Access Journals (Sweden)

    Wu Bi

    2016-03-01

    Full Text Available To find novel functional beverages from folk teas, 33 species of frequently used non-Camellia tea (plants other than Camellia were collected and compared with Camellia tea (green tea, pu-erh tea and black tea for the first time. Data are reported here on the quantities of 20 free amino acids (FAAs and three purine alkaloids (measured by UHPLC, total polyphenols (measured by Folin-Ciocalteu assay, and antioxidant activity (DPPH. The total amounts of FAAs in non-Camellia tea (0.62–18.99 mg/g are generally less than that of Camellia tea (16.55–24.99 mg/g. However, for certain FAAs, the quantities were much higher in some non-Camellia teas, such as γ-aminobutyric acid in teas from Ampelopsis grossedentata, Isodon serra and Hibiscus sabdariffa. Interestingly, theanine was detected in tea from Potentilla fruticosa (1.16±0.81 mg/g. Furthermore, the content of polyphenols in teas from A. grossedentata, Acer tataricum subsp. ginnala are significantly higher than those from Camellia tea; teas from I. serra, Pistacia chinensis and A. tataricum subsp. ginnala have remarkable antioxidant activities similar to the activities from green tea (44.23 μg/mL. Purine alkaloids (caffeine, theobromine and theophylline were not detected in non-Camellia teas. The investigation suggest some non-Camellia teas may be great functional natural products with potential for prevention of chronic diseases and aging, by providing with abundant polyphenols, antioxidants and specific FAAs.

  5. Investigation of free amino acid, total phenolics, antioxidant activity and purine alkaloids to assess the health properties of non-Camellia tea.

    Science.gov (United States)

    Bi, Wu; He, Chunnian; Ma, Yunyun; Shen, Jie; Zhang, Linghua Harris; Peng, Yong; Xiao, Peigen

    2016-03-01

    To find novel functional beverages from folk teas, 33 species of frequently used non-Camellia tea (plants other than Camellia) were collected and compared with Camellia tea (green tea, pu-erh tea and black tea) for the first time. Data are reported here on the quantities of 20 free amino acids (FAAs) and three purine alkaloids (measured by UHPLC), total polyphenols (measured by Folin-Ciocalteu assay), and antioxidant activity (DPPH). The total amounts of FAAs in non-Camellia tea (0.62-18.99 mg/g) are generally less than that of Camellia tea (16.55-24.99 mg/g). However, for certain FAAs, the quantities were much higher in some non-Camellia teas, such as γ-aminobutyric acid in teas from Ampelopsis grossedentata, Isodon serra and Hibiscus sabdariffa. Interestingly, theanine was detected in tea from Potentilla fruticosa (1.16±0.81 mg/g). Furthermore, the content of polyphenols in teas from A. grossedentata, Acer tataricum subsp. ginnala are significantly higher than those from Camellia tea; teas from I. serra, Pistacia chinensis and A. tataricum subsp. ginnala have remarkable antioxidant activities similar to the activities from green tea (44.23 μg/mL). Purine alkaloids (caffeine, theobromine and theophylline) were not detected in non-Camellia teas. The investigation suggest some non-Camellia teas may be great functional natural products with potential for prevention of chronic diseases and aging, by providing with abundant polyphenols, antioxidants and specific FAAs. PMID:27006902

  6. Microwave-assisted extraction of apocynum tea polyphenols%微波辅助提取罗布麻茶茶多酚的工艺研究

    Institute of Scientific and Technical Information of China (English)

    王伟; 訾王贝; 樊海燕; 杨晓玲

    2012-01-01

    Apocynum is a kind of rare wild plants, and mainly grows in the desert saline and alkaline land or bank, valley, hillside sandy earth, such as in north China. Xinjiang desert region has the best quality of apocynum. Tea polyphenol in apocynum consists of flavanones, anthocyanin compounds, flavonoids, flavonol and phenolic acids, etc. Among them flavanol (catechin) is the most important compound in forming the tea color and flavor. It is also one of the health functions ingredients in apocynum tea. In this study, microwave assisted extraction was used in extracting polyphenols from Xinjiang apocynum tea. Through single factor experiment and orthogonal test, optimization extractive conditions were obtained. The optimal extraction conditions were: 50% ethanol concentration, 1/25 material liquid ratio, 70 C for 90s, and 500W microwave power. Under these conditions, the extraction quantity and extraction rate of tea polyphenols were 12. 52mg · g-1and 91. 52% , respectively.%罗布麻又名野麻,是一种世界上稀有的野生植物,它主要生长在沙漠盐碱地或河岸、山沟、山坡的沙质地上,在我国北方大多省区都有生长,尤其以新疆沙漠地区的罗布麻品质最佳.茶多酚是罗布麻茶叶中多酚类物质的总称,包括黄烷醇类、花色苷类、黄酮类、黄酮醇类和酚酸类等,其中以黄烷醇类物质(儿茶素)最为重要,是形成罗布麻茶叶色香味的主要成份之一,也是罗布麻茶叶中有保健功能的主要成份之一.因此本文以新疆罗布麻茶为原料,采用微波辅助提取茶多酚,通过单因素实验和正交试验优化提取条件.结果表明,从罗布麻中提取茶多酚的最佳条件为:乙醇浓度50%、料液比1∶25、温度70℃、提取时间90s、微波功率500W,此条件下茶多酚提取量为12.52mg·g-1,提取率为91.52%.

  7. PLGA-encapsulated tea polyphenols enhance the chemotherapeutic efficacy of cisplatin against human cancer cells and mice bearing Ehrlich ascites carcinoma

    Science.gov (United States)

    Singh, Madhulika; Bhatnagar, Priyanka; Mishra, Sanjay; Kumar, Pradeep; Shukla, Yogeshwer; Gupta, Kailash Chand

    2015-01-01

    The clinical success of the applicability of tea polyphenols awaits efficient systemic delivery and bioavailability. Herein, following the concept of nanochemoprevention, which uses nanotechnology for enhancing the efficacy of chemotherapeutic drugs, we employed tea polyphenols, namely theaflavin (TF) and epigallocatechin-3-gallate (EGCG) encapsulated in a biodegradable nanoparticulate formulation based on poly(lactide-co-glycolide) (PLGA) with approximately 26% and 18% encapsulation efficiency, respectively. It was observed that TF/EGCG encapsulated PLGA nanoparticles (NPs) offered an up to ~7-fold dose advantage when compared with bulk TF/EGCG in terms of exerting its antiproliferative effects and also enhanced the anticancer potential of cisplatin (CDDP) in A549 (lung carcinoma), HeLa (cervical carcinoma), and THP-1 (acute monocytic leukemia) cells. Cell cycle analysis revealed that TF/EGCG-NPs were more efficient than bulk TF/EGCG in sensitizing A549 cells to CDDP-induced apoptosis, with a dose advantage of up to 20-fold. Further, TF/EGCG-NPs, alone or in combination with CDDP, were more effective in inhibiting NF-κB activation and in suppressing the expression of cyclin D1, matrix metalloproteinase-9, and vascular endothelial growth factor, involved in cell proliferation, metastasis, and angiogenesis, respectively. EGCG and TF-NPs were also found to be more effective than bulk TF/EGCG in inducing the cleavage of caspase-3 and caspase-9 and Bax/Bcl2 ratio in favor of apoptosis. Further, in vivo evaluation of these NPs in combination with CDDP showed an increase in life span (P<0.05) in mice bearing Ehrlich’s ascites carcinoma cells, with apparent regression of tumor volume in comparison with mice treated with bulk doses with CDDP. These results indicate that EGCG and TF-NPs have superior cancer chemosensitization activity when compared with bulk TF/EGCG. PMID:26586942

  8. Synergistic anticancer activity of dietary tea polyphenols and bleomycin hydrochloride in human cervical cancer cell: Caspase-dependent and independent apoptotic pathways.

    Science.gov (United States)

    Alshatwi, Ali A; Periasamy, Vaiyapuri Subbarayan; Athinarayanan, Jegan; Elango, Ramesh

    2016-03-01

    Bleomycin is a chemotherapeutic agent that is frequently used in the treatment of various cancers. Bleomycin causes serious adverse effects via antioxidant defense abnormalities against reactive oxygen species (ROS). However, the current cervical cancer monodrug therapy strategy has failed to produce the expected outcomes; hence, combinational therapies are gaining great interest. Tea polyphenols are also effective antioxidative and chemo-preventive agents. However, the combined effect of tea polyphenol (TPP) and bleomycin (BLM) against cervical cancer remains unknown. In this study, we focused on the potential of TPP on BLM anticancer activity against cervical cancer cells. Cervical cancer cells (SiHa) were treated with various concentrations of TPP, BLM and TPP combined with BLM (TPP-BLM), and their effects on cell growth, intracellular reactive oxygen species, poly-caspase activity, early apoptosis and the expression of caspase-3, caspase-8 and caspase-9, Bcl-2 and p53 were assessed. The MTT assay revealed that the SiHa cells were less sensitive to growth inhibition by TPP treatment compared with both BLM and the combination therapy. Nuclear staining indicated that exposure to TPP-BLM increased the percentage of apoptotic nuclei compared with a mono-agent treatment. Caspase activation assay demonstrated that proportion of early and late apoptotic/secondary necrotic cells was higher in the cells treated with the combination therapy than in those treated with either TPP or BLM alone. The TPP-BLM treatment synergistically induced apoptosis through caspase-3, caspase-8 and caspase-9 activation, Bcl-2 upregulation and p53 overexpression. This study suggests that TPP-BLM may be used as an efficient antioxidant-based combination therapy for cervical cancer. PMID:26800624

  9. Green tea and risk of breast cancer in Asian Americans.

    Science.gov (United States)

    Wu, Anna H; Yu, Mimi C; Tseng, Chiu-Chen; Hankin, Jean; Pike, Malcolm C

    2003-09-10

    There is substantial in vitro and in vivo evidence implicating tea polyphenols as chemopreventive agents against various cancers. However, epidemiologic data obtained from mainly Western populations are not supportive of a protective role of tea, mainly black tea, in the etiology of breast cancer. Much less is known about the relationship between green tea and breast cancer risk. During 1995-1998, we conducted a population-based, case-control study of breast cancer among Chinese, Japanese and Filipino women in Los Angeles County and successfully interviewed 501 breast cancer patients and 594 control subjects. Detailed information on menstrual and reproductive factors; dietary habits, including intake of black and green tea; and other lifestyle factors was collected. Risk of breast cancer was not related to black tea consumption. In contrast, green tea drinkers showed a significantly reduced risk of breast cancer, and this was maintained after adjusting for age, specific Asian ethnicity, birthplace, age at menarche, parity, menopausal status, use of menopausal hormones, body size and intake of total calories and black tea. Compared to women who did not drink green tea regularly (i.e., less than once a month), there was a significant trend of decreasing risk with increasing amount of green tea intake, adjusted odds ratios being 1.00, 0.71 (95% confidence interval [CI] 0.51-0.99) and 0.53 (95% CI 0.35-0.78), respectively, in association with no, 0-85.7 and >85.7 ml of green tea per day. The significant inverse association between risk of breast cancer and green tea intake remained after further adjustment for other potential confounders, including smoking; alcohol, coffee and black tea intake; family history of breast cancer; physical activity; and intake of soy and dark green vegetables. While both green tea and soy intake had significant, independent protective effects on breast cancer risk, the benefit of green tea was primarily observed among subjects who were low

  10. Synergistic Application of Black Tea Extracts and Lactic Acid Bacteria in Protecting Human Colonocytes against Oxidative Damage.

    Science.gov (United States)

    Zhao, Danyue; Shah, Nagendra P

    2016-03-23

    In view of the potential of lactic acid bacteria (LAB) to enhance the antioxidant activity of food products, this work explored the effectiveness of LAB fermented black tea samples in alleviating H2O2-induced oxidative stress in human colonocytes. The antioxidant capacity of tea samples was evaluated in terms of cyto-protectiveness, mitochondria membrane potential (Δψm)-stabilizing activity, ROS-inhibitory effect, and antioxidant enzyme-modulating activity. The effect on oxidative DNA damage and repair was studied in CCD 841 by comet assay. Results showed that the protective effect of tea pretreatment was more pronounced in normal cells (CCD 841) than in carcinomas (Caco-2), and fermented samples were invariably more effective. Higher cell viability and Δψm were maintained and ROS production was markedly inhibited with tea pretreatment. The fermented tea samples also remarkably stimulated DNA repair, resulting in fewer strand breaks and oxidative lesions. Our study implied that LAB fermentation may be an efficient way to enhance the antioxidative effectiveness of black tea flavonoid-enriched foods. PMID:26790920

  11. 茶多酚对肥胖及糖尿病的预防干预作用%Tea polyphenols in the prevention and the treatment of obesity and diabetes

    Institute of Scientific and Technical Information of China (English)

    钱晓娟

    2011-01-01

    茶多酚(tea polyphenols,TP)是从天然植物茶叶中提取的多酚化合物复合体,具有较好的抗氧化清除自由基的能力.近年来研究表明,TP具有降血压、调节血脂、降血糖、预防心血管病、预防癌症、抗辐射损伤和防衰延寿等作用.文中主要综述TP对肥胖与糖尿病的作用.%Tea polyphenols are a class of complex compounds extracted from tea, with fine anti-oxidant and free radical scavenging ability. Recent studies show that tea polyphenols have a lot of functions, as lowering blood pressure, regulating blood lipid and blood sugar, preventing cardiovascular disease and cancer, anti-radiacion damage , anti-senility, and so on. This review focuses on its role in the prevention and the treatment of obesity and diabetes.

  12. The Design and Implementation of Black Tea Traceability System Based on UHF RFID

    Institute of Scientific and Technical Information of China (English)

    Caijin; LING; Jinxin; LIU; Jiayao; WU; Qiushuang; WANG; Shumei; LIU; Haiqiang; CHEN; Chaoyi; ZHAO

    2014-01-01

    In order to realize the whole process of consumer- producer bidirectional traceability and distinguish the true from the false,according to Yinghong 9,planting,processing and circulation situation,we introduce UHF RFID and Network information technology,to design and develop the UHF RFID black tea traceability system.The system adopts.NET technology,uses Microsoft Visual Studio 2010 + VB.NET,Microsoft SQL SERVER 2005,T-SQL language for development and design,and uses the network architecture of C /S combined with B /S,to randomly generate ID with 20 bit code and label,thereby forming the only corresponding relation and pointing to the corresponding batch and product types.The technology mode using three-layer structure for development realizes the functions of data collection,tracing,sharing and query.The data and business processing are concentrated on the server,simplifying the development,use and maintenance of system.We build and implement Yinghong 9 black tea traceability system,to the needs of government,the public and the producers for Yinghong 9 brand protection,product quality tracking and market regulation.

  13. Tea and human health: biomedical functions of tea active components and current issues.

    Science.gov (United States)

    Chen, Zong-mao; Lin, Zhi

    2015-02-01

    Originating in China, tea and tea planting have spread throughout the world since the middle of the Tang dynasty. Now people from 160 countries in the world are accustomed to tea drinking. A brief history of tea's medicinal role in China and its spread to the world are introduced. The effectiveness of tea active components and tea drinking on major human diseases, including cancer, metabolic syndrome, cardiovascular disease, and neurodegenerative diseases, is discussed. Also presented are some related issues, such as the bioavailability of tea active components, the new formulations of tea polyphenols, and the safety for consumers of dietary supplements containing tea polyphenols. PMID:25644464

  14. Green Tea Extract Ameliorates Learning and Memory Deficits in Ischemic Rats via Its Active Component Polyphenol Epigallocatechin-3-gallate by Modulation of Oxidative Stress and Neuroinflammation

    Directory of Open Access Journals (Sweden)

    Kuo-Jen Wu

    2012-01-01

    Full Text Available Ischemic stroke results in brain damage and behavioral deficits including memory impairment. Protective effects of green tea extract (GTex and its major functional polyphenol (−-epigallocatechin gallate (EGCG on memory were examined in cerebral ischemic rats. GTex and EGCG were administered 1 hr before middle cerebral artery ligation in rats. GTex, EGCG, and pentoxifylline (PTX significantly improved ishemic-induced memory impairment in a Morris water maze test. Malondialdehyde (MDA levels, glutathione (GSH, and superoxide dismutase (SOD activity in the cerebral cortex and hippocampus were increased by long-term treatment with GTex and EGCG. Both compounds were also associated with reduced cerebral infraction breakdown of MDA and GSH in the hippocampus. In in vitro experiments, EGCG had anti-inflammatory effects in BV-2 microglia cells. EGCG inhibited lipopolysaccharide- (LPS- induced nitric oxide production and reduced cyclooxygenase-2 and inducible nitric oxide synthase expression in BV-2 cells. GTex and its active polyphenol EGCG improved learning and memory deficits in a cerebral ischemia animal model and such protection may be due to the reduction of oxidative stress and neuroinflammation.

  15. Tea Effects and Landscape Design of Tea Garden

    OpenAIRE

    Yue Liu; Bingqing Yang

    2015-01-01

    This study analyzes tea effects via the study of the influence of tea polyphenol on rats. Having summarized and generalized the landscape design of tea culture garden, the study ends up with explicit significance of tea garden. During the research, the paper finds that lacking facilities, garden zones are not fully fictionalized with common and ordinary landscapes and not obviously characterized by local cultures. To solve mentioned problems, it is proposed that tea gardens should break throu...

  16. The green synthesis of gold nanoparticles using the ethanol extract pf black tea and its tannin free fraction

    International Nuclear Information System (INIS)

    In this research the ethanol extract of black tea and its tannin free fraction used for green synthesis of gold nanoparticles. All the extracts were used separately for the synthesis of gold nanoparticles through the reduction of aqueous AuCl4-. Transmission electron microscopy and visible absorption spectroscopy confirmed the reduction of gold ions to gold nanoparticles. The ethanol extract of black tea and its tannin free ethanol extract produced gold nanoparticles in the size ranges of 2.5-27.5 nm and 1.25-17.5 nm with an average size of 10 nm and 3 nm, respectively. The prepared colloid gold nanoparticles, using the ethanol extract of black tea, did not show the appropriate stability during storage time (24 hours) at 4degC. In contrast, gold colloids, which were synthesized by a tannin free fraction showed no particle aggregation during short and long storage times at the same conditions. To the best of our knowledge, this is the first report on the rapid synthesis of gold nanoparticles using ethanol extract of black tea and its tannin free fraction.

  17. In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.

    Directory of Open Access Journals (Sweden)

    Wanigasekera Daya Ratnasooriya

    2014-12-01

    Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation.

  18. LC-DAD-ESI-MS/MS characterization of phenolic constituents in Turkish black tea: Effect of infusion time and temperature.

    Science.gov (United States)

    Kelebek, Hasim

    2016-08-01

    The influence of different infusion times and temperatures on phenolic, antioxidant, and color properties of black teas (Camellia sinensis L.) was investigated in the present study. The teas were prepared using infusion times of 3, 6, and 10min at 80 and 100°C. Liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS/MS), whereby the two purine alkaloids were detected using positive ESI, and the other 33 phenolic compounds were detected using negative ESI. A total of 35 phenolic compounds were identified and quantified in the tea infusions, with the most abundant being gallic acid, (-)-epicatechin-gallate, 4-p-coumaroylquinic acid, quercetin-3-O-galactosyl-rhamnosyl-glucoside, kaempferol-3-O-glucosyl-rhamnosyl-glucoside, theaflavin, and theobromine. The antioxidant capacity of the tea infusions was determined using two different methods: DPPH (2,2-diphenyl-1-picryl hydrazyl) and ABTS (2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulfonic acid) assays. DPPH and the ABTS values varied largely among the tea preparations. The increment in infusion times and temperature led to increase in the tea's antioxidant capacities. PMID:26988497

  19. Batch fermentation of black tea by kombucha: A contribution to scale-up

    Directory of Open Access Journals (Sweden)

    Malbaša Radomir V.

    2005-01-01

    Full Text Available Local domestic Kombucha was used in fermentation of 1.5 g L-1 of black tea (Indian tea, " Vitamin ", Horgoš, Serbia and Montenegro, sweetened with approximately 70 g L'1 of sucrose. Inoculation was performed either with 10% or 15% (v/v of fermentation broth from previous process. The fermentation was conducted in geometrically similar vessels with 0.4 L, 0.8 L, 4 L and 8 L of substrate, at 22±1 °C for 28 days. The samples were analyzed after 3, 4 5, 6, 7, 10, 14 and 28 days, so that their pH values, content of total acids sucrose, glucose and fructose contents, as well as contents of ethanol and vitamin C were determined. Based on the experiment design, the response surface for the product pH, as a function of time, beverage volume and inoculum concentration, was defined in the form of a second-order polynomial. From the obtained response surface, a formula for scaling-up of the process was derived.

  20. Black tea (camellia sinensis ) role in modulating antioxidant enzymes and biochemical changes in γ -irradiated rats

    International Nuclear Information System (INIS)

    In the present work, we investigated the efficacy of camellia sinensis beverage in reducing gamma-irradiation - induced oxidative damage to the liver, lipid profile and antioxidant enzymes in adult male rats. Animals were received the black tea beverage (BTB) as a sole source of potable liquid for seven consecutive days before exposing them to single dose of 6 Gy whole-body gamma irradiation . The irradiated rats continued to receive BTB for 21 days before sacrifice. The effect of BTB was assessed by monitoring the plasma aspartate transaminase (AST), alanine transaminase (ALT), acid phosphatase (AcP), total cholesterol (TC), triglycerides (TG), high, low and very low density lipoproteins cholesterol (HDL-C,LDL-C and VLDL-C) as well as protein carbonyl content (PCC). In addition, the concentration of thiobarbituric acid-reactive substances (TBARS), reduced glutathione (GSH) and catalase activity (CAT) in blood and liver of experimental rats. It was observed that tea administration lowers significantly (p< 0.05), the plasma AST, ALT, AcP activities and TC, TG, LDL-C, VLDL-C and PCC concentration as well as blood and liver TBARS. The level of GSH and activity of CAT in the blood and liver tissue were however shown to be significantly elevated (p< 0.05).The results provide useful information for future investigations and strongly implicate the beneficial application of BTB

  1. Chemical characterization of heteropolysaccharides from green and black teas (Camellia sinensis) and their anti-ulcer effect.

    Science.gov (United States)

    Scoparo, Camila T; Souza, Lauro M; Dartora, Nessana; Sassaki, Guilherme L; Santana-Filho, Arquimedes P; Werner, Maria Fernanda P; Borato, Débora G; Baggio, Cristiane H; Iacomini, Marcello

    2016-05-01

    In order to obtain polysaccharides from green and black teas (Camellia sinensis), commercial leaves were submitted to infusion and then to alkaline extraction. The extracts were fractionated by freeze-thawing process, giving insoluble and soluble fractions. Complex arabinogalactan protein from the soluble fractions of both teas (GTPS and BTPS) were determined by methylation analysis and (1)H/(13)C-HSQC spectroscopy, showing a main chain of (1→3)-β-Galp, substituted at O-6 by (1→6)-linked β-Galp with side chains of α-Araf and terminal units of α-Araf, α-Fucp and α-Rhap. A highly branched heteroxylan from the insoluble fractions (GTPI and BTPI) showed in methylation analysis and (1)H/(13)C-HSQC spectroscopy the main chain of (1→4)-β-Xylp, substituted in O-3 by α-Araf, β-Galp and α-Glcp units. Evaluating their gastroprotective activity, the fractions containing the soluble heteropolysaccharides from green (GTPS) and black teas (BTPS) reduced the gastric lesions induced by ethanol. Furthermore, the fraction of insoluble heteropolysaccharides of green (GTPI) and black (BTPI) teas also protected the gastric mucosa. In addition, the maintenance of gastric mucus and reduced glutathione (GSH) levels was involved in the polysaccharides gastroprotection. PMID:26861826

  2. [Rapid and Dynamic Determination Models of Amino Acids and Catechins Concentrations during the Processing Procedures of Keemun Black Tea].

    Science.gov (United States)

    Ning, Jing-ming; Yan, Ling; Zhang, Zheng-zhu; Wei, Ling-dong; Li, Lu-qing; Fang, Jun-ting; Huang, Cai-wang

    2015-12-01

    Tea is one of the most popular beverages in the world. For the contribution to the taste and healthy functions of tea, amino acids and catechins are important components. Among different kinds of black teas in the world, Keemun black tea has the famous and specific fragrance, "Keemun aroma". During the processing procedure of Keemun black tea, the contents of amino acids and catechins changed greatly, and the differences of these concentrations during processing varied significantly. However, a rapid and dynamic determination method during the processing procedure was not existed up to now. In order to find out a rapid determination method for the contents of amino acids and catechins during the processing procedure of Keemun black tea, the materials of fresh leaves, withered leaves, twisted leaves, fermented leaves, and crude tea (after drying) were selected to acquire their corresponding near infrared spectroscopy and obtain their contents of amino acids and catechins by chemical analysis method. The original spectra data were preprocessed by the Standard Normal Variate Transformation (SNVT) method. And the model of Near Infrared (NIR) spectroscopy with the contents of amino acids and catechins combined with Synergy Interval Partial Least squares (Si-PLS) was established in this study. The correlation coefficients and the cross validation root mean square error are treated as the efficient indexes for evaluating models. The results showed that the optimal prediction model of amino acids by Si-PLS contained 20 spectral intervals combined with 4 subintervals and 9 principal component factors. The correlation coefficient and the root mean square error of the calibration set were 0. 955 8 and 1. 768, respectively; the correlation coefficient and the root mean square error of the prediction set were 0. 949 5 and 2. 16, respectively. And the optimal prediction model of catechins by Si-PLS contained 20 spectral intervals combined with 3 subintervals and 10 principal

  3. 木瓜蛋白酶对白茶浸提液中茶多酚含量的影响%Effects of Papain on the Extractive Content of White Tea Polyphenols

    Institute of Scientific and Technical Information of China (English)

    赵文净; 刘祖锋

    2015-01-01

    In this study, the effects on the extractive content of white tea polyphenols was investigated by adding papain, changing extraction temperature and time. The factors which had influenced on the extraction content of tea polyphenols was studied by single factors and orthogonal experiment. The results showed that the sequence of influencing factors was as follows: extraction temperature, extraction time, papain dosage; The optimum process was as follows:extraction temperature 65 ℃, extraction time 40 min, papain dosage 3 %. Under this condition, the extractive content of white tea polyphenols was up to 8.31 g/kg.%通过对浸提温度、浸提时间、酶添加量进行单因素和正交试验,探讨木瓜蛋白酶辅助浸提法对白茶茶多酚浸提含量的影响.结果表明,影响白茶茶多酚浸出量的主次顺序为浸提温度、浸提时间、酶添加量;最佳工艺为浸提温度65℃,浸提时间为40 min,酶添加量为3%,此条件下白茶茶多酚浸出量为8.31 g/kg.

  4. Formation of enriched black tea extract loaded chitosan nanoparticles via electrospraying

    Science.gov (United States)

    Hammond, Samuel James

    Creating nanoparticles of beneficial nutraceuticals and pharmaceuticals has had a large surge of research due to the enhancement of absorption and bioavailability by decreasing their size. One of these ways is by electrohydrodynamic atomization, also known as electrospraying. In general, this novel process is done by forcing a liquid through a capillary nozzle and which is subjected to an electrical field. While there are different ways to create nanoparticles, the novel method of electrospraying can be beneficial over other types of nanoparticle formation. Reasons include high control over particle size and distribution by altering electrospray parameters (voltage, flow rate, distance, and time), higher encapsulation efficiency than other methods, and also it is a one step process without exposure to extreme conditions (Gomez-Estaca et. al. 2012, Jaworek and Sobcyzk 2008). The current study aimed to create a chitosan encapsulated theaflavin-2 enriched black tea extract (BTE) nanoparticles via electrospraying. The first step of this process was to create the smallest chitosan nanoparticles possible by altering the electrospray parameters and the chitosan-acetic acid solution parameters. The solution properties altered include chitosan molecular weight, acetic acid concentration, and chitosan concentration. Specifically, the electrospray parameters such as voltage, flow rate and distance from syringe to collector are the most important in determining particle size. After creating the smallest chitosan particles, the TF-2 enriched black tea extract was added to the chitosan-acetic acid solution to be electrosprayed. The particles were assessed with the following procedures: Atomic force microscopy (AFM) and scanning electron microscopy (SEM) for particle morphology and size, and loading efficiency with ultraviolet--visible spectrophotometer (UV-VIS). Chitosan-BTE nanoparticles were successfully created in a one step process. Diameter of the particles on average

  5. Drinking Green Tea Modestly Reduces Breast Cancer Risk12

    OpenAIRE

    Shrubsole, Martha J.; Lu, Wei; Chen, Zhi; Shu, Xiao Ou; Zheng, Ying; Dai, Qi; Cai, Qiuyin; Gu, Kai; Ruan, Zhi Xian; Gao, Yu-Tang; Zheng, Wei

    2009-01-01

    Green tea is a commonly consumed beverage in China. Epidemiological and animal data suggest tea and tea polyphenols may be preventive against various cancers, including breast cancer. Catechol-O-methyltransferase (COMT) catalyzes catechol estrogens and tea polyphenols. The COMT rs4680 AA genotype leads to lower COMT activity, which may affect the relationship between green tea consumption and breast cancer risk. We evaluated whether regular green tea consumption was associated with breast can...

  6. Synergistic Antioxidant Activity of Sweet Potato Extracts in Combination with Tea Polyphenols and Pueraria Flavonoid in Vitro

    OpenAIRE

    Xiaojuan Liu; Fenglin He; Lichao Zhao; Aimei Zhou; Xin Liu

    2014-01-01

    The antioxidant activity of Sweet Potato Extracts (SPE) can be enhanced by the presence of these other active antioxidants such as Tea Polyphones (TP) and Pueraria Flavonoid (PF). Since many of these natural antioxidants are consumed together in foods, the potential for synergistic interactions is high in the human diet. The aim of this study was to determine what concentrations and combinations of antioxidants among SPE, TP and PF are capable of producing synergistic antioxidant effects, bas...

  7. Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model

    Directory of Open Access Journals (Sweden)

    Noguchi Takashi

    2010-11-01

    Full Text Available Abstract Background In our previous study, allogeneic-transplanted peripheral nerve segments preserved for one month in a polyphenol solution at 4°C could regenerate nerves in rodents demonstrated the same extent of nerve regeneration as isogeneic fresh nerve grafts. The present study investigated whether the same results could be obtained in a canine model. Methods A sciatic nerve was harvested from a male beagle dog, divided into fascicules of Sry and β-actin to investigate whether cells of donor origin remained in the allogeneic nerve segments. FK506 concentration was measured in blood samples taken before the animals were killed. Results The total myelinated axon numbers and amplitudes of the muscle action potentials correlated significantly with the blood FK506 concentration. Few axons were observed in the allogeneic-transplanted nerve segments in the PA0.025 group. PCR showed clear Sry-specific bands in specimens from the PA0.1 and PA0.05 groups but not from the PA0.025 group. Conclusions Successful nerve regeneration was observed in the polyphenol-treated nerve allografts when transplanted in association with a therapeutic dose of FK506. The data indicate that polyphenols can protect nerve tissue from ischemic damage for one month; however, the effects of immune suppression seem insufficient to permit allogeneic transplantation of peripheral nerves in a canine model.

  8. Rapid and direct volatile compound profiling of black and green teas (Camellia sinensis) from different countries with PTR-ToF-MS.

    Science.gov (United States)

    Yener, Sine; Sánchez-López, José A; Granitto, Pablo M; Cappellin, Luca; Märk, Tilmann D; Zimmermann, Ralf; Bonn, Günther K; Yeretzian, Chahan; Biasioli, Franco

    2016-05-15

    Volatile profiles of 63 black and 38 green teas from different countries were analysed with Proton Transfer Reaction-Time of Flight-Mass Spectrometry (PTR-ToF-MS) both for tea leaves and tea infusion. The headspace volatile fingerprints were collected and the tea classes and geographical origins were tracked with pattern recognition techniques. The high mass resolution achieved by ToF mass analyser provided determination of sum formula and tentative identifications of the mass peaks. The results provided successful separation of the black and green teas based on their headspace volatile emissions both from the dry tea leaves and their infusions. The volatile fingerprints were then used to build different classification models for discrimination of black and green teas according to their geographical origins. Two different cross validation methods were applied and their effectiveness for origin discrimination was discussed. The classification models showed a separation of black and green teas according to geographical origins the errors being mostly between neighbouring countries. PMID:26992494

  9. Binding Inhibition Between Igf1r and Igf1 by Catechin of Black Tea

    Directory of Open Access Journals (Sweden)

    Lina Firdausi

    2012-12-01

    Full Text Available The natural compound of black tea is used as an alternative of obesity therapies in the world; particularly, the catechin family in tea leaves which has bioactive compounds such as EC, EGC and EGCG. Their bioactivity contributes to inhibit the ligand of Insulin-Like Growth Factor I Receptor (Igf-1r binding-region to Igf-1 protein. To elucidate the inhibiton of Igf-1 expression and proliferating of Rattus norvegicus strain wistar adipose cell using black tea solution. The research used Rattus norvegicus strain wistar. After a 90-day treatment, the adipose tissues were picked up from the viscera of each experimental animal, and then the adipose tissues were embedded by paraffin. The paraffin sections were determined through immunohistochemistry with anti-Igf-1 antiserum, and were also analyzed through hematoxylin-eosin. A protein sequence of Igf-1, Igf-1r, and 3D structure of EC, EGC and EGCG from Gene Bank sites were used during in silico analysis. The sequences were aligned by BLAST program to identify the conserve and variable domain of IGF-1 protein isoforms. The 3D structures of IGF-1 and IGF-1R were constructed using Phyre program. The ligand among the 3D structures of IGF-1, IGF-1R and catechin compounds were analyzed using Hex 5.1 docking program. The data showed that the Igf-1 expression of adipose cells was reduced at 0,03 g/ml BTS and 0,045 g/ml BTS treatments. The result of BLAST analysis showed that IGF-1 (a, b, c, and d isoforms conserved a domain from amino acid no 22 until 134; and this region was a variable region. The EGCG bound L1 domain of IGF-1R with E-total -235.3 KJ/mol which was lower than EC (-208,4 KJ/mol and EGC (-142 KJ/mol. The total energy of IGF-1 (a, b, c, but not d isoform which interacted with EGCG was around -223.7 KJ/mol, EC is -205.6 KJ/mol and EGC was -191.7 KJ/mol. However, EC, EGC and EGCG was only able to prevent the interaction between the L1 of IGF-1R with IGF-1 protein, but not the opposite.

  10. Optimization of Enzymatic Extraction for Tea Polyphenols from Sweet Tea by Response Surface Methodology%响应面分析法优化酶提取甜茶茶多酚工艺

    Institute of Scientific and Technical Information of China (English)

    程雅芳; 杨洋; 温富雄; 袁经权; 缪剑华

    2012-01-01

    利用响应面分析法对复合酶辅助提取甜茶中的茶多酚的工艺进行优化。在单因素试验基础上选取因素与水平,根据中心组合的试验设计原理和响应面分析法,分析各个因素的显著性和交互作用,结果确定甜茶中的茶多酚的提取最佳工艺条件为:复合酶是由纤维素酶和果胶酶以3:4的比例混合而成;在45℃的水浴条件下,加酶量为0.6%(m/m)、pH4.95、酶解时间47.76min、料液比1:23.58(g/mL),酶解后的原料用体积分数40%的乙醇溶液、料液比1:28(g/mL)、温度70℃回流提取70min的条件下,茶多酚提取量可达133.2mg/g。%The extraction process of polyphenols from sweet tea was explored using compound enzyme-assisted extraction.The compound enzymes were composed of cellulose and pectinase at a mixture ratio of 3:4.Based on response surface methodology,the optimal extraction conditions of enzyme-assisted were enzyme dosage of 0.6%,hydrolysis temperature of 45℃,extraction pH of 4.95,extraction time of 47.76 min,and solid-liquid ratio of 1:23.58.The ethanol extraction conditions were ethanol concentration of 40%(V/V),extraction time of 70 min,extraction temperature of 70℃ and solid-liquid radio of 1:28.Under the optimal extraction conditions,the yield of tea polyphenols was 133.2 mg/g.

  11. 修剪物与茶多酚对茶树矿质吸收及根系有机酸分泌的影响%Effects of Tea Pruning Materials and Tea Polyphenols on Organic Acids Secretion and Mineral Uptake in Tea Plant

    Institute of Scientific and Technical Information of China (English)

    庞鑫; 王玉花; 王伟东; 尹盈; 疏再发; 陈暄; 黎星辉

    2014-01-01

    The effects of aqueous solution of tea pruning materials and tea polyphenols on tea plant [Camellia sinensis (L.)] growth and the mechanisms were investigated using hydroponic culture in th is paper. The plants were harvested and root exudates were collected after growing periods of 15, 35, 55, 75 and 95 d. The contents of organic acids in the secretes from roots were determined using a HPLC equipped with C18 column after passed through cation and anion exchange resin and concentrated. Besides, the dry weight, tea polyphenols and mineral uptake were analyzed. The results showed that tea polyphenols added in nutrient solution inhibited the uptake of Ca, Mg, Fe, Mn, Zn and growth rate and induced citric and succinic acids efflux from roots. Ho wever, the aqueous solution of tea pruning materials added in nutrient solution promoted the uptake of K, Ca, Mg, Fe, Mn, Zn, Al and growth rate and at the meantime induced oxalic, malic, citric and succinic acids efflux from roots. Besides, the aqueous so lution of tea pruning materials also lowered pH of collection solution, which were closely linked with organic acids secretion changes, hinting tea pruning materials may result in soil acidification.%探究茶树修剪物水溶性成分及茶多酚对茶树生长的影响与作用机制。水培茶苗,于15、35、55、75、95 d收集根系分泌物,通过阴、阳离子树脂纯化、减压浓缩后,利用高效液相色谱(HPLC)测定分泌物中有机酸主要组成与含量。同时测定茶苗干物质量、茶多酚及矿质元素含量等指标。研究表明,茶多酚会抑制茶树对 Ca、Mg、Fe、Mn、Zn 的吸收,抑制生长,同时诱导茶树根系分泌苹果酸、柠檬酸;适量修剪物水溶物能够补充矿质营养,提高茶树K、Ca、Fe、Mn、Zn、Al的吸收,促进生长,但同时也会诱导草酸、苹果酸、柠檬酸和琥珀酸的分泌,降低收集液 pH值,而收集液 pH值变化与有机酸分泌量变化显著

  12. A Systematic Review on Exposure to Toxic and Essential Elements through Black Tea Consumption in Iran: Could It be a Major Risk for Human Health?

    Directory of Open Access Journals (Sweden)

    Elahe Rezaee

    2014-01-01

    Conclusions: The hazard of excessive element intake through black tea consumption should be considered as negligible in Iran. However, related risk for manganese appeared to be more than toxic metals.

  13. Effect of black tea on enteral feeding tolerance in ICU patients

    OpenAIRE

    Mojdeh, Soheila; Shahin, Samire; Khalili, Gholamreza

    2010-01-01

    BACKGROUND: Tea consumption has been known mostly as a well-drink after water in the world. Tea drink can affect balance of fluids and renal function. In addition, it can cause loss of many viruses in the stomach and can increase or decrease gastrointestinal movements. This research was done to determine the effect of tea on increasing enteral feeding tolerance in ICU patients in Alzahra Hospital. METHODS: In this clinical trial study, 45 patients were enrolled in two groups, tea consumption ...

  14. 绿茶和红茶提取物抑制中波紫外线诱导HaCaT细胞氧化损伤和凋亡的比较%Comparation the inbibitional effects between extracts of green tea and black tea on oxidative damage and apoptosis of HaCaT cells induced by UVB

    Institute of Scientific and Technical Information of China (English)

    马蕊; 刘仲华; 黄建安; 林勇; 陈金华; 钟源

    2013-01-01

    To compare the photo-protective effect of green and black tea extracts on HaCaT cells damaged from ultraviolet radiation B.Sub-confluent HaCaT cells were incubated for 6 h with different doses of extracts from green tea and black tea,and then irradiated with 60 mJ/cm2 doses of UVB.The change of the cell viability,the activity of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),lactate dehydrogenase (LDH),and the level of malondialdehyde (MDA) of supernatant were compared.The content of ROS was measured with the fluorescence method.Annexin V/PI double staining was performed to evaluate the change of apoptosis using flow cytometry.In addition,the contents of tea polyphenols and catechins from extracts of green and black tea were also tested.The results were as follows:Compared with control group,the UVB irradiation could seriously harm HaCaT cell and gave rise to 21.61% cell viability decline.Extracts form green and black tea could enhance cell viability,SOD and GSH-Px activity in supernatant under UVB irradiation,and decrease LDH activity,the content of MDA and the content of ROS in cells in dose-dependent manner (P <0.01).Compared with UVB-irradiated model group,the apoptosis rate of HaCaT cell in other groups was decreased by 6.94% and 3.68% respectively.Moreover,the photo-protective effect of green tea extracts was better than that of black tea extracts,which were consistent with the content of tea polyphenols and catechins their contained.Green and black tea extracts could relieve UVB-induced oxidative damage and apoptosis of HaCaT cells,which might be the reason that they raised the oxidase activity and cleared the oxy-radicals in cells.Moreover,the photo-protective effect of green tea extracts against oxidative damage and apoptosis of HaCaT cells from UVB irradiation was better than that of black tea extracts.%为比较绿茶、红茶提取物对中波紫外线(UVB)诱导角质形成细胞光损伤的抑制作用,用不同浓度的绿茶、

  15. Inhibitory effects of polyphenol-enriched extract from Ziyang tea against human breast cancer MCF-7 cells through reactive oxygen species-dependent mitochondria molecular mechanism

    Directory of Open Access Journals (Sweden)

    Wenfeng Li

    2016-07-01

    Full Text Available A polyphenol-enriched extract from selenium-enriched Ziyang green tea (ZTP was selected to evaluate its antitumor effects against human breast cancer MCF-7 cells. In ZTP, (−-epigallocatechin gallate (28.2% was identified as the major catechin, followed by (−-epigallocatechin (5.7% and (−-epicatechin gallate (12.6%. ZTP was shown to inhibit MCF-7 cell proliferation (half maximal inhibitory concentration, IC50 = 172.2 μg/mL by blocking cell-cycle progression at the G0/G1 phase and inducing apoptotic death. Western blotting assay indicated that ZTP induced cell-cycle arrest by upregulation of p53 and reduced the expression of CDK2 in MCF-7 cells. ZTP-caused cell apoptosis was associated with an increase in Bax/Bcl-2 ratio, and activation of caspase-3 and -9. MCF-7 cells treated with ZTP also showed an overproduction of reactive oxygen species, suggesting that reactive oxygen species played an important role in the induction of apoptosis in MCF-7 cells. This is the first report showing that ZTP is a potential novel dietary agent for cancer chemoprevention or chemotherapy.

  16. Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants

    Institute of Scientific and Technical Information of China (English)

    ZHANG Junwei; WANG Yan; PENG Qijun

    2013-01-01

    Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactant two-phase system (ASTP) with cationic suffactant (CTAB) and anionic surfactant (SDS).Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant.The increase in concentrations of NaBr and Na2SO4 are beneficial to the formation of ASTP.Theanine concentration in the bottom phase increases with increasing concentration of theanine,whereas the partition coefficient and extraction rate only change a little when the concentration of theanine is above 0.2 g· L-1.With the increase of SDS concentration,the phase ratio and the partition coefficient decrease,while the extraction efficiency of theanine increases and the concentration of theaninc changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio.The temperature has a notable effect on the concentration of theanine in the bottom phase,partition coefficient and extraction rate of theanine.The increase of waste liquid decreases the phase ratio,increases the concentration and extraction rate of theanine in the bottom phase,since the protein and the saccharide enter the bottom phase with theanine.

  17. (--Epicatechin-3-gallate, a Green Tea Polyphenol Is a Potent Agent Against UVB-induced Damage in HaCaT Keratinocytes

    Directory of Open Access Journals (Sweden)

    Chi-Feng Hung

    2007-08-01

    Full Text Available (--Epicatechin-3-gallate (ECG is a polyphenolic compound similar to (--epigallocatechin-3-gallate (EGCG which is abundant in green tea. Numerous workers have proposed that EGCG protects epidermal cells against UVB-induced damage. However, little has been known about whether ECG protects keratinocytes against UVB-induced damage. We decided to investigate the protective effects and underlying mechanisms of ECG on UVB-induced damage. Cell viability was determined by the MTT assay. Activation of ERK1/2, p38 and JNK was analyzed by Western blotting. Intracellular H2O2 production and DNA content was analyzed by flow cytometry. Lipid peroxidation was assayed by colorimetry. In our study, we found that ECG dose-dependently attenuated UVB-induced keratinocyte death. Moreover, ECG markedly inhibited UVB-induced cell membrane lipid peroxidation and H2O2 generation in keratinocytes, suggesting that ECG can act as a free radical scavenger when keratinocytes were photodamaged. In parallel, H2O2-induced the activation of ERK1/2, p38 and JNK in keratinocytes could be inhibited by ECG. UVB-induced pre-G1 arrest leading to apoptotic changes of keratinocytes were blocked by ECG. Taken together, we provide here evidence that ECG protects keratinocytes from UVB-induced photodamage and H2O2-induced oxidative stress, possibly through inhibition of the activation of ERK1/2, p38 and JNK and/or scavenging of free radicals.

  18. Effect of tea polyphenols on production performance, egg quality, and hepatic antioxidant status of laying hens in vanadium-containing diets.

    Science.gov (United States)

    Yuan, Z H; Zhang, K Y; Ding, X M; Luo, Y H; Bai, S P; Zeng, Q F; Wang, J P

    2016-07-01

    This study was conducted to determine the effect of tea polyphenols (TP) on production performance, egg quality, and hepatic-antioxidant status of laying hens in vanadium-containing diets. A total of 300 Lohman laying hens (67 wk old) were used in a 1 plus 3 × 3 experiment design in which hens were given either a diet without vanadium and TP supplementation (control) or diets supplemented with 5, 10, or 15 mg V/kg and TP (0, 600, 1,000 mg/kg) diets for 8 wk, which included 2 phases: a 5-wk accumulation phase and a 3-wk depletion phase. During the accumulation phase, dietary vanadium addition decreased (linear, P hens fed 15 mg/kg vanadium and 600 mg/kg TP showed no difference from the control diet only after 1 wk withdrawal. In the liver, the activity of glutathione S-transferases and glutathione peroxidase was increased (linear, P laying hens from the adverse effect of vanadium on egg quality, liver antioxidant stress and shorten the recovery time. PMID:27044874

  19. In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)

    Institute of Scientific and Technical Information of China (English)

    Wanigasekera Daya Ratnasooriya

    2014-01-01

    To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase. Results: The results revealed that BTB had moderate [IC50=(1.09±0.12) mg/mL] and dose dependent (r2=0.94) anti-hyaluronidase activity. EGCG also exhibited dose dependent (r2=0.93, P<0.05) anti-hyaluronidase activity which was superior [IC50=(0.09±0.00) mg/mL] to BTB. Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation.

  20. Green tea and prevention of esophageal and lung cancers

    OpenAIRE

    Yuan, Jian-Min

    2011-01-01

    Green tea contains high concentrations of tea polyphenols that have shown inhibitory effects against the development, progress, and growth of carcinogen-induced tumors in animal models at different organ sites, including the esophagus and lung. Green tea polyphenols also have shown to suppress cell proliferation and induce apoptosis. Besides antioxidative property, green tea polyphenols have pro-oxidative activities under certain conditions and modulate phase II metabolic enzymes that can enh...

  1. Bioaccessibility of Ca, Mg, Mn and Cu from whole grain tea-biscuits: Impact of proteins, phytic acid and polyphenols.

    Science.gov (United States)

    Vitali, D; Vedrina Dragojević, I; Šebečić, B

    2008-09-01

    Levels of some essential minerals (Ca, Mg, Cu and Mn) were determined in ten different types of experimentally prepared hard biscuits. In relation to the wheat flour-based reference sample, other investigated samples were enriched with different ratios of integral raw materials of different origin or various dietary fibers in view of improving their functionality and nutritive quality. The goal of the research was to evaluate enriched biscuits as additional sources of calcium, magnesium, copper and manganese in nutrition and to investigate if the modifications of wheat flour based biscuit composition significantly change the amounts of total and bioaccessible minerals in the final product. Since our results indicated significant changes of mineral bioaccessibility among the samples, obtained results were correlated to the content of proteins, phytic acid and polyphenols for the sake of assessing their impact as limiting factors of mineral bioaccessibility in these types of foods. PMID:26050166

  2. Effects of γ-radiation on microbial load and antioxidant proprieties in black tea irradiated with different water activities

    International Nuclear Information System (INIS)

    The aim of this paper is to study the effect of gamma radiation on black tea irradiated with different water activities. The black tea samples had their Aw adjusted to three values (0.92, 0.65, and 0.18) and were irradiated in 60Co source at doses of 0, 1.0, 1.5, 2.0, 2.5, 5.0, 7.5, and 10.0 kGy. The methods used were: microbiology, total phenolic compounds quantification, antioxidant activity by ORAC, and quantification of the main antioxidants. It was observed that the greater the amount of free water present in the samples, lower was the dose to achieve microbiological control. Regardless the water activity used, there was no difference in content of the phenolic compounds and at the mainly theaflavins, as well in the antioxidant activity at doses up to 5.0 kGy. - Highlights: • Higher the Aw, lower is the radiation dose to archive microbiology safety. • The doses up to 10.0 kGy had no effect on antioxidant capacity in all Aw used. • The recommended dose to irradiated black tea is 5.0 kGy

  3. Kinetic and Thermodynamic Studies of the Adsorption of Crystal Violet onto Used Black Tea Leaves

    Directory of Open Access Journals (Sweden)

    Mohammad Abul Hossain

    2013-11-01

    Full Text Available This study presents the kinetic and thermodynamic investigation of the adsorption of crystal violet (CV on used black tea leaves (UBTL from aqueous solution to evaluate the feasibility of the process. The effects of concentration, solution pH and temperature on adsorption kinetics were carried out in batch process. Kinetic studies have shown that the adsorption data partially follow simple first order, second order and pseudo second order kinetic equations for different initial concentrations at pH 2.0. The equilibrium amount adsorbed, equilibrium concentration and rate constant were calculated from better fitted pseudo second order kinetic plots for different initial concentrations. The equilibrium amount adsorbed (200 mg/g at 30 oC increased with the increase of temperature, indicated endothermic nature of the adsorption. The apparent activation energy of adsorption was determined from Arrhenius plot using pseudo second order rate constant and the value, Ea = 83.1 kJ/mol, revealed the process is chemisorption. Thermodynamic parameters: DHo, DGo and DSo, were determined from the equilibrium adsorption constant and the results obtained confirmed that the adsorption process was feasible, less spontaneous and endothermic. The equilibrium amount adsorbed was found to be increased with increase of solution pH from 2.0 to 6.0 indicating electrostatic interaction between cationic CV with anionic surface of UBTL dominated at higher pH due to the low zero point charge of pH of UBTL.

  4. Adsorption kinetics of Rhodamine-B on used black tea leaves

    Directory of Open Access Journals (Sweden)

    Hossain Mohammad

    2012-08-01

    Full Text Available Abstract Rhodamine B (Rh-B is one of the most common pollutants in the effluents of textile industries effluents in developing countries. This study was carried out to evaluate the applicability of used black tea leaves (UBTL for the adsorptive removal of Rh-B from aqueous system by investigating the adsorption kinetics in batch process. The effects of concentration and temperature on adsorption kinetics were examined. First-, second- and pseudo-second order kinetic equations were used to investigate the adsorption mechanism. The adsorption of Rh-B on UBTL followed pseudo-second order kinetics. The equilibrium amount adsorbed and the equilibrium concentration were calculated from pseudo-second-order kinetic plots for different initial concentrations of Rh-B to construct the adsorption isotherm. The adsorption isotherm was well expressed by Langmuir equation. The maximum adsorption capacity of UBTL to Rh-B was found to be 53.2 mg/g at pH = 2.0. The equilibrium amount adsorbed, calculated from pseudo-second-order kinetic plots, increased with temperature increase. The positive value of enthalpy of adsorption, ΔHads = 31.22 kJ/mol, suggested that the adsorption of Rh-B on UBTL at pH = 2.0 is an endothermic process.

  5. Fractionation and identification of minor and aroma-active constituents in Kangra orthodox black tea.

    Science.gov (United States)

    Joshi, Robin; Gulati, Ashu

    2015-01-15

    The aroma constituents of Kangra orthodox black tea were isolated by simultaneous distillation extraction (SDE), supercritical fluid extraction and beverage method. The aroma-active compounds were identified using gas chromatography-olfactometry-mass spectrometry. Geraniol, linalool, (Z/E)-linalool oxides, (E)-2-hexenal, phytol, β-ionone, hotrienol, methylpyrazine and methyl salicylate were major volatile constituents in all the extracts. Minor volatile compounds in all the extracts were 2-ethyl-5-methylpyrazine, ethylpyrazine, 2-6,10,14-trimethyl-2-pentadecanone, acetylfuran, hexanoic acid, dihydroactinidiolide and (E/Z)-2,6-nonadienal. The concentrated SDE extract was fractionated into acidic, basic, water-soluble and neutral fractions. The neutral fraction was further chromatographed on a packed silica gel column eluted with pentane and diethyl ether to separate minor compounds. The aroma-active compounds identified using gas chromatography-olfactometry-mass spectrometry were 2-amylfuran, (E/Z)-2,6-nonadienal, 1-pentanol, epoxylinalool, (Z)-jasmone, 2-acetylpyrrole, farnesyl acetone, geranyl acetone, cadinol, cubenol and dihydroactinidiolide. AEDA studies showed 2-hexenal, 3-hexenol, ethylpyrazine, (Z/E)-linalool oxides, linalool, (E/Z)-2,6-nonadienal, geraniol, phenylethanol, β-ionone, hotrienol and dihydroactinidiolide to be odour active components. PMID:25148991

  6. Gas chromatographic method for the determination of hexaconazole residues in black tea

    Institute of Scientific and Technical Information of China (English)

    Chinnachamy KARTHIKA; Paul James SACHIN

    2008-01-01

    A highly reliable, quantitative and sensitive analytical method for determining the residues of the fungicide, hexaconazole in black tea is described. The proposed method is based on liquid-liquid extraction followed by gas chromatographic determination, using nitrogen phosphorus detector (GC-NPD) for the identification and quantitation of hexaconazole. The most was cleaned up by adsorption column chromatography using activated florisil. Performance of the method was assessed by evaluating quality parameters such as recovery value, repeatability, reproducibility, linearity and limits of detection and quantitation. When the method was assessed for repeatability, the percentage of recovery ranged between 86% and 96% while the relative standard deviation was between 0.30% and 2.35%. In studies on reproducibility the recovery ranged from 81% to 85% and relative standard deviation from 1.68% to 5.13%, implying that the method was reliable. A field trial was conducted to verify the application of this method with real samples. Results prove that the validated method was suitable for extracting hexaconazole residues.

  7. Equilibrium adsorption of rhodamine B on used black tea leaves from acidic aqueous solution

    Directory of Open Access Journals (Sweden)

    Mohammad Abul Hossain

    2012-10-01

    Full Text Available The presence of carcinogenic dye like rhodamine B (Rh-B in textile wastewater affects the quality of water to consumers. The adsorption of Rh-B on used black tea leaves (UBTL was studied in batch process to investigate its removal efficiency. The effects of contact time, concentration, temperature, pH etc. on adsorption have been investigated. The UV-visible spectrophotometer was used for analysis of Rh-B at constant pH. The adsorption isotherms were constructed for different temperatures using acidic solution of pH 2.0. Freundlich, Langmuir and Dubinin–Raduskevich (D-R equations were used to analyze the equilibrium adsorption data. The experimental data follows Freundlich equation more precisely compare with the Langmuir one. The maximum amount adsorbed calculated from Langmuir equation is 72.5 mg/g at 30 oC which is increased with increasing temperature. Separation factor and thermodynamic parameters revealed that the process is favorable, spontaneous and endothermic nature. Possible mechanism of the process was elucidated from the effect of solution pH on amount adsorbed. The endothermic nature of the adsorption might be due to the fragmentation of Rh-B molecules during the adsorption process.

  8. Efficacy of methanolic extract of green and black teas against extended-spectrum β-Lactamase-producing Pseudomonas aeruginosa.

    Science.gov (United States)

    Taherpour, Arezou; Hashemi, Ali; Erfanimanesh, Soroor; Taki, Elahe

    2016-07-01

    Pseudomonas aeruginosa is one of the major bacteria causing acute infections. β-Lactamase production is the principal defense mechanism in gram-negative bacteria. The aim of our study was to evaluate the antibacterial activity of Methanolic Extracts of Green and Black Teas on P. aeruginosa Extended Spectrum-β-Lactamases (ESBLs) production. This research was carried out on burn wounds of 245 hospitalized patients in Kerman, Iran. P. aeruginosa ESBLs and MBL producing strains were detected by Combination Disk Diffusion Test (CDDT) and Epsilometer test (E-test) strips, respectively. Minimum inhibitory concentration (MIC) was measured for Ceftazidime, Meropenem, Imipenem, Aztreonam, Cefotaxime and methanollic extracts of Camellia Sinensis (Green Tea). From 245 patients in the burn ward, 120 cases were infected with P. aeruginosa. 41 isolates contained ESBL while MBL was not detected. P. aeruginosa were resistant to Cefotaxime, Aztreonam, Ceftazidime, Meropenem and Imipenem, 72 (60%), 50 (41.66%), 79 (65.83%), 33 (27.5%) and 24 (20%), respectively. Green tea extract had the highest anti-bacterial effect on standard and P. aeruginosa strains in 1.25mg/ml concentration. This study determined that the methanolic extract of green tea has a higher effect against ESBL producing P. aeruginosa than Cefotaxime, Aztreonam and Ceftazidime. PMID:27393439

  9. Pharmacokinetic and Chemoprevention Studies on Tea in Humans

    OpenAIRE

    Chow, H-H. Sherry; Hakim, Iman A.

    2011-01-01

    Green tea and its major polyphenols constituents, tea catechins, have been shown to have many health benefits including cancer prevention. Tea catechins and tea catechin metabolites/catabolites are bioavailable in the systemic circulation after oral intake of green tea or green tea catechins. The metabolites/catabolites identified in humans include glucuronide/sulfate conjugates, methylated tea catechin conjugates, and microflora-mediated ring fission products and phenolic acid catabolites. P...

  10. Root canal filling material added with tea polyphenols inhibits the generation of Candida albicans biofilm%根管充填材料加入茶多酚抑制白色念珠菌生物膜的生成

    Institute of Scientific and Technical Information of China (English)

    许颖; 吕庆; 康梁; 张慧明

    2013-01-01

    BACKGROUND: Candida albicans biofilms can influence the prognosis of root canal fil ing, and tea polyphenols can in vitro inhibit the Candida albicans biofilm, while the antibacterial mechanism may play the role through influencing or interfering the expression of resistance gene. OBJECTIVE: To investigate the inhibitory effect of root canal fil ing material added with tea polyphenols on Candida albicans biofilm. METHODS: Constant broth dilution method was used to determine the minimal inhibitory concentration of tea polyphenols in vitro inhibited Candida albicans biofilms, in order to identify the inhibitory effect. Then the expressions of Candida albicans resistance genes CDR1, CDR2 and MDR1 were detected with reverse transcriptase polymerase chain reaction after inhibited with tea polyphenols. RESULTS AND CONCLUSION: The minimum inhibitory concentration of tea polyphenols used to inhibit Candida albicans biofilm was 11.5 mg/mL. Reverse transcriptase polymerase chain reaction results showed that expressions of related resistance genes CDR1 and CDR2 were decreased with the gradient increasing of drug concentration, and when the concentration reached to 25 mg/mL or above, the mRNA expression level was completely inhibited, while the concentration of the resistance drug has less effect on the expression of resistance drug MDR1. Tea polyphenols has inhibitory effect on Candida albicans biofilm, and has significant inhibitory effect on the expressions of experimental selected resistance genes CDR1 and CDR2.%  背景:白色念珠菌生物膜是根管充填治疗预后的影响因素,茶多酚体外可能对白色念珠菌生物膜有抑菌作用,其抑菌机制可能通过影响或干扰相关耐药基因的表达而实现。目的:探讨根管充填材料加入茶多酚对白色念珠菌生物膜的抑制作用。方法:采用常量肉汤稀释法确定茶多酚体外对白色念珠菌生物膜的最小抑菌浓度,以此判断抑菌效果,然后用

  11. The biochemistry and technology of tea manufacture.

    Science.gov (United States)

    Bokuchava, M A; Skobeleva, N I

    1980-01-01

    This review surveys tea-production technology, chemistry of raw and manufactured tea as well as tea tasting appraisal and chemical analysis of manufactured tea. The paper describes the healthful properties of tea, gives general information on the tea plant and manufactured tea, and presents classification of teas as related to the processing of black green, yellow, and red tea, green pressed tea as well as instant tea and tea dyes. The paper discusses the chemical composition of raw and manufactured tea as well as approaches to the evaluation of tea quality--tea tasting appraisal and chemical analysis. The paper is supplied with the conclusions and references. The section on the healthful properties of tea discusses various aspects of catechin effects--vitamin P, antimicrobial, antioxidative and radioprotective effects. Also described are favorable effects of tea alkaloids--caffeine, theobromine, theophylline that dilatate cerebral vessels and alleviate headaches. The section on the production of different teas (black, green, yellow, red, instant teas, and tea dyes) considers technological methods and biochemical bases of various types of tea manufacture. The role of tea leaf enzymes in the oxidative processes determining the tea quality is discussed in detail. This section also describes the contribution of thermochemical processes into the formation of tea flavor. The compounds dictating tea taste and aroma are discussed, particularly tannins and catechins, volatile oils, nitrogen compounds, and some other substances. PMID:6996921

  12. Role of dietary polyphenols in attenuating brain edema and cell swelling in cerebral ischemia

    Science.gov (United States)

    Polyphenols are natural substances with variable phenolic structures and are enriched in vegetables, fruits, grains, bark, roots, tea, and wine. There are over 8000 polyphenolic structures identified in plants, but edible plants contain only several hundred polyphenolic structures. Recent interest...

  13. Green tea polyphenols change the profile of inflammatory cytokine release from lymphocytes of obese and lean rats and protect against oxidative damage.

    Science.gov (United States)

    Molina, N; Bolin, A P; Otton, R

    2015-10-01

    This study aimed to investigate whether green tea polyphenols (GT) modulate some functional parameters of lymphocytes from obese rats. Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight) and obesity was induced by cafeteria diet (8 weeks). Lymphocytes were obtained from mesenteric lymph nodes for analyses. In response to the cafeteria diet we observed an increase in activity of the metabolic enzyme hexokinase, ROS production, MnSOD, CuZnSOD and GR enzyme activities and proliferation capacity of the cells (baseline), whereas IL-10 production was decreased. Obese rats treated with GT decreased cell proliferation (under ConA stimulation). Hexokinase and G6PDH activity, ROS production and MnSOD, CuZnSOD, GPx and GR enzymes remained increased, accompanied by an increase in Nrf2 mRNA level. There was a decrease in pro-inflammatory IL-2, IL-6, IL-1β, TNF-α cytokines that were accompanied by a decrease in the mRNA level of TRL4 while IL-10 production was increased in obese rats treated with GT. GT treatment of lean rats showed similar results to that of obese rats treated with GT, indicating that the effects of GT are independent of diet. Foxp3 and IRF4 mRNA levels were increased by GT. In conclusion, cafeteria diet modulated the function of lymphocytes from lymph nodes, increasing ROS production and decreasing anti-inflammatory IL-10, which could contribute to the inflammatory state in obesity. GT reduced ROS production, improving the redox status and reducing pro-inflammatory cytokine production by lymphocytes, suggesting that GT treatment may be driving lymphocytes to a more anti-inflammatory than pro-inflammatory microenvironment. PMID:26299975

  14. Green tea polyphenols reduced fat deposits in high fat-fed rats via erk1/2-PPARγ-adiponectin pathway.

    Directory of Open Access Journals (Sweden)

    Chong Tian

    Full Text Available OBJECTIVE: Hypoadiponectinemia contributes to the development of obesity and related disorders such as diabetes, hyperlipidemia, and cardiovascular diseases. In this study we investigated the effects of green tea polyphenols (GTPs on adiponectin levels and fat deposits in high fat (HF fed rats, the mechanism of signaling pathway was explored as well. METHODS AND RESULTS: Male Wistar rats were fed with high-fat diet. GTPs (0.8, 1.6, 3.2 g/L were administered via drinking water. Serum adiponectin and insulin were measured by ELISA, mRNA levels of adiponectin and PPARγ in visceral adipose tissue (VAT were determined by Real-time PCR, protein levels of PPARγ, phospho (p - PPARγ, extracellular signal regulated kinase (erk 1/2 and p-erk1/2 in VAT were determined by western blot. GTPs treatment attenuated the VAT accumulation, hypoadiponectinemia and the decreased mRNA level of adiponectin in VAT induced by HF. Decreased expression and increased phosphorylation of PPARγ (the master regulator of adiponectin, and increased activation of erk1/2 were observed in HF group, and these effects could be alleviated by GTPs treatment. To explore the underlying mechanism, VAT was cultured in DMEM with high glucose to mimic the hyperglycemia condition in vitro. Similar to the results of in vivo study, decreased adiponectin levels, decreased expression and increased phosphorylation of PPARγ, and elevated erk1/2 phosphorylation in cultured VAT were observed. These effects could be ameliorated by co-treatment with GTPs or PD98059 (a selective inhibitor of erk1/2. CONCLUSION: GTPs reduced fat deposit, ameliorated hypoadiponectinemia in HF-fed rats, and relieved high glucose-induced adiponectin decrease in VAT in vitro. The signaling pathway analysis indicated that PPARγ regulation mediated via erk1/2 pathway was involved.

  15. Effects of long-term tea polyphenols consumption on hepatic microsomal drug-metabolizing enzymes and liver function in Wistar rats

    Institute of Scientific and Technical Information of China (English)

    Tao-Tao Liu; Ning-Sheng Liang; Yan Li; Fan Yang; Yi Lu; Zi-Qing Meng; Li-Sheng Zhang,

    2003-01-01

    AIM: To investigate the effects of long-term tea polyphenols (TPs) consumption on hepatic microsomal drug-metabolizing enzymes and liver function in rats.METHODS: TPs were administered intragastrically to rats at the doses of 833 mg.kg-1.d-1 (n=20) and 83.3 mg.kg-1@d-1 (n=20) respectively for six months. Controlled group (n=20)was given same volume of saline solution. Then the contents of cytochrome P450, bS, enzyme activities of aminopyrine N-demethylase (ADM), glutathione S-trasferase (GST) and the biochemical liver function of serum were determined.RESULTS: The contents of cytochrome P450 and b5 in the livers of male rats in high dose groups (respectively 2.66±0.55,10.43±2.78 nmol.mg MS pro-1) were significantly increased compared with the control group (1.08±1.04, 5.51±2.98nmol.mg MS pro-1; P<0.01, respectively). The enzymatic activities of ADM in the livers of female rats in high dose groups (0.91±0.08 mmol@mg MS pro-1min-1) were increased compared with the control group (0.82±0.08 mmol.mg MS pro-1.min-1; P<0.05). The GST activity was unchanged in all treated groups, and the function of liver was not obviously changed.CONCLUSION: The antidotal capability of rats' livers can be significantly improved after long-term consumption of TPs.There are differences in changes of drug-metabolizing enzymes between the sexes induced by TPs and normal condition.

  16. High-dose green tea polyphenol intake decreases CYP3A expression in a liver-specific manner with increases in blood substrate drug concentrations.

    Science.gov (United States)

    Ikarashi, Nobutomo; Ogawa, Sosuke; Hirobe, Ryuta; Kusunoki, Yoshiki; Kon, Risako; Ochiai, Wataru; Sugiyama, Kiyoshi

    2016-06-30

    In recent years, the intake of functional foods containing high-doses of green tea polyphenols (GP) has been increasing. In this study, the long-term safety of high-dose GP was assessed from a pharmacokinetic point of view by focusing on the drug-metabolizing enzyme, cytochrome P450 (CYP). Mice were fed a diet containing 3% GP for 4weeks, and the CYP expression levels and activity were determined. The GP-treated group showed a significant decrease in the hepatic CYP3A and an increase in the hepatic CYP2C expression compared with the control group. CYP1A, CYP2D, and CYP2E expression were not different between the GP-treated and the control groups. In the small intestine, there were no differences in the CYP3A protein levels between the groups. The increase in the plasma triazolam concentration in the GP-treated group was observed. Although no changes were found in the hepatic CYP3A levels in mice receiving a diet containing 0.1% GP for 4weeks, a significant decrease was seen in the hepatic CYP3A level in mice receiving a diet containing 3% GP for only 1week. This study revealed that the intake of a high-dose GP results in a liver-specific decrease in the CYP3A expression level. The results also indicated that the effects of GP on CYP3A were not observed following the intake of a low-dose GP. In the future, caution should be taken in cases when functional foods containing a high-dose GP are concomitantly consumed with a CYP3A substrate drug. PMID:27130545

  17. Green tea polyphenols-induced apoptosis in human osteosarcoma SAOS-2 cells involves a caspase-dependent mechanism with downregulation of nuclear factor-κB

    International Nuclear Information System (INIS)

    Development of chemotherapy resistance and evasion from apoptosis in osteosarcoma, a primary malignant bone tumor, is often correlated with constitutive nuclear factor-κB (NF-κB) activation. Here, we investigated the ability of a polyphenolic fraction of green tea (GTP) that has been shown to have antitumor effects on various malignant cell lines to inhibit growth and induce apoptosis in human osteosarcoma SAOS-2 cells. Treatment of SAOS-2 cells with GTP (20-60 μg/ml) resulted in reduced cell proliferation and induction of apoptosis, which correlated with decreased nuclear DNA binding of NF-κB/p65 and lowering of NF-κB/p65 and p50 levels in the cytoplasm and nucleus. GTP treatment of cells reduced IκB-α phosphorylation but had no effect on its protein expression. Furthermore, GTP treatment resulted in the inhibition of IKK-α and IKK-β, the upstream kinases that phosphorylate IκB-α. The increase in apoptosis in SAOS-2 cells was accompanied with decrease in the protein expression of Bcl-2 and concomitant increase in the levels of Bax. GTP treatment of SAOS-2 cells also resulted in significant activation of caspases as was evident by increased levels of cleaved caspase-3 and caspase-8 in these cells. Treatment of SAOS-2 cells with a specific caspase-3 inhibitor Ac-Asp-Glu-Val-Asp-CHO (Ac-DEVD-CHO) and general caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp (OMe)-fluoromethyl ketone (Z-VAD-FMK) rescued SAOS-2 cells from GTP-induced apoptosis. Taken together, these results indicate that GTP is a candidate therapeutic for osteosarcoma that mediates its antiproliferative and apoptotic effects via activation of caspases and inhibition of NF-κB

  18. Green tea polyphenol epigallocatechin-3-gallate inhibits TLR4 signaling through the 67-kDa laminin receptor on lipopolysaccharide-stimulated dendritic cells

    International Nuclear Information System (INIS)

    Highlights: ► Expressions of CD80, CD86, and MHC class I/II were inhibited by EGCG via 67LR. ► EGCG-treated DCs inhibited LPS-induced pro-inflammatory cytokines via 67LR. ► EGCG-treated DCs inhibited MAPKs activation and NF-κB p65 translocation via 67LR. ► EGCG elevated the expression of the Tollip protein through 67LR in DCs. -- Abstract: Epigallocatechin-3-gallate (EGCG), a major active polyphenol of green tea, has been shown to down-regulate inflammatory responses in dendritic cells (DCs); however, the underlying mechanism has not been understood. Recently, we identified the 67-kDa laminin receptor (67LR) as a cell-surface EGCG receptor. In this study, we showed the molecular basis for the down-regulation of toll-like receptor 4 (TLR4) signal transduction by EGCG in DCs. The expressions of CD80, CD86, and MHC class I and II, which are molecules essential for antigen presentation by DCs, were inhibited by EGCG via 67LR. In addition, EGCG-treated DCs inhibited lipopolysaccharide (LPS)-induced production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6) and activation of mitogen-activated protein kinases (MAPKs), e.g., extracellular signal-regulated kinase 1/2 (ERK1/2), p38, c-Jun N-terminal kinase (JNK), and nuclear factor κB (NF-κB) p65 translocation through 67LR. Interestingly, we also found that EGCG markedly elevated the expression of the Tollip protein, a negative regulator of TLR signaling, through 67LR. These novel findings provide new insight into the understanding of negative regulatory mechanisms of the TLR4 signaling pathway and consequent inflammatory responses that are implicated in the development and progression of many chronic diseases.

  19. Anti-Inflammatory Effects of a Polyphenols-Rich Extract from Tea (Camellia sinensis) Flowers in Acute and Chronic Mice Models

    OpenAIRE

    Bang-Tian Chen; Wei-Xi Li; Rong-Rong He; Yi-Fang Li; Bun Tsoi; Yu-Jia Zhai; Hiroshi Kurihara

    2012-01-01

    While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis) flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE) could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected against Propionibacterium acnes ...

  20. Tea aroma formation

    Directory of Open Access Journals (Sweden)

    Chi-Tang Ho

    2015-03-01

    Full Text Available Besides water, tea is one of the most popular beverages around the world. The chemical ingredients and biological activities of tea have been summarized recently. The current review summarizes tea aroma compounds and their formation in green, black, and oolong tea. The flavor of tea can be divided into two categories: taste (non-volatile compounds and aroma (volatile compounds. All of these aroma molecules are generated from carotenoids, lipids, glycosides, etc. precursors, and also from Maillard reaction. In the current review, we focus on the formation mechanism of main aromas during the tea manufacturing process.

  1. 茶多酚的功效特性及其在军用食品中的开发应用%Functions of Tea Polyphenol and Its Applications in Military Food

    Institute of Scientific and Technical Information of China (English)

    裴晨琛; 程鹏

    2011-01-01

    茶多酚具有抑菌、抗病毒、免疫调节、抗氧化等诸多功能,可在军用食品中开发保健食品、抗氧化剂、防腐剂、食用色素及稳定剂等,应用前景广阔.%Tea polyphenol has many functions such as bacteriostasic activity, anti-viral effect, immunoregulative effect and oxidation resistance. Its applications in military food were introduced, including health food, antioxidant, antiseptic, edible pigment and color stability.

  2. 茶多酚对运动大鼠抗疲劳作用的实验研究%The experimental study about anti-fatigue effect of the tea polyphenol on the movement of rats

    Institute of Scientific and Technical Information of China (English)

    杜云

    2012-01-01

    目的 通过实验观察补充茶多酚提取物对SD大鼠运动疲劳的作用.方法 将50只雄性SD大鼠随机分为5组:安静组和模型组不给药,每天蒸馏水灌胃;高、中、低剂量组分别给予300,150,50 mg/kg的茶多酚提取物.30 d后,检测大鼠跑台力竭时间、运动后血乳酸水平、乳酸脱氢酶活力和血清尿素氮水平.结果 茶多酚提取物可增加大鼠力竭时间,提高运动后大鼠血乳酸脱氢酶活力,同时降低大鼠运动后血乳酸及尿素氮水平,尤以300 mg/kg剂量的效果最明显.结论 实验结果说明茶多酚提取物对运动大鼠有明显的抗疲劳作用.%Aim To experimentally observe the effect of the tea polyphenol extract on SD rats in terms of the sports fatigue. Methods 50 male SD rats were randomly divided into 5 groups: quiet group and model group daily tap water lavage; high, medium, low dose groups were given 300,150, 50 mg/kg tea polyphenol extract. After 30 days, the rats' treadmill exhaustive time, blood lactic acid after exercise level, lactic acid dehydrogenase activity and serum urea nitrogen level were detected. Results Tea polyphenol extracts can increase rats' exhaustive time, improve the blood lactate dehydrogenase activity, while reducing the rats after-exercise blood lactic acid and blood urea nitrogen levels, especially in the 300 mg/kg dose group, the effect is the most obvious. Conclusion The ex-periment results show that tea polyphenol extract has obvious anti-fatigue effect.

  3. Multivariate data reduction and discrimination of black and green teas due to the physical fractionation pattern of selected metals determined in their infusions.

    Science.gov (United States)

    Pohl, Pawel; Szymczycha-Madeja, Anna; Stelmach, Ewelina; Welna, Maja

    2016-11-01

    An analytical scheme for the physical fractionation of Al, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Sr and Zn in black and green teas infusions was proposed. It was based on the ultrafiltration/centrifugation through five membranes having molecular weight cut-offs of 100, 50, 30, 10 and 5kDa. The concentrations of the studied metals in the highest and the lowest molecular weight fractions were found to classify and discriminate the analyzed teas infusions much better than the total metals concentrations. The differences in the physical fractionation patterns of these metals assessed for both tea varieties was profound and let to simply classify the infusions of different black and green teas by principal component analysis and linear discriminant analysis. PMID:27591619

  4. The Involvement of a Polyphenol-Rich Extract of Black Chokeberry in Oxidative Stress on Experimental Arterial Hypertension

    Directory of Open Access Journals (Sweden)

    Manuela Ciocoiu

    2013-01-01

    Full Text Available The aim of this study is to characterize the content of Aronia melanocarpa Elliott (black chokeberry extract and also to estimate the influence of polyphenolic compounds contained in chokeberries on oxidative stress, on an L-NAME-induced experimental model of arterial hypertension. The rat blood pressure values were recorded using a CODA Noninvasive Blood Pressure System. HPLC/DAD coupled with ElectroSpray Ionization-Mass Spectrometry allowed identification of five phenolic compounds in berries ethanolic extract as follows: chlorogenic acid, kuromanin, rutin, hyperoside, and quercetin. The serous activity of glutathione-peroxidase (GSH-Px has significantly lower values in the hypertensive (AHT group as compared to the group protected by polyphenols (AHT + P. The total antioxidant capacity (TAC values are lower in the AHT group and they are significantly higher in the AHT + P group. All the measured blood pressure components revealed a biostatistically significant blood pressure drop between the AHT group and the AHT + P group. The results reveal the normalization of the reduced glutathion (GSH concentration as well as a considerable reduction in the malondialdehyde (MDA serum concentration in the AHT + P group. Ethanolic extract of black chokeberry fruits not only has a potential value as a prophylactic agent but also may function as a nutritional supplement in the management of arterial hypertension.

  5. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    Science.gov (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver. PMID:25837382

  6. Inhibition of salivary amylase by black tea in high-caries and low-caries index children: A comparative in vivo study

    OpenAIRE

    Arya, Vishal; Taneja, Lavina

    2015-01-01

    Introduction: Dental caries is a universal disease. Dietary modification is important in reducing the occurrence of dental caries. Tea which is so frequently consumed with cariogenic starch rich food is proposed to have anticariogenic potential. The various mechanism has been proposed for same and one being inhibition of salivary amylase activity. Aim: To determine the effect of 1.5% black tea decoction on salivary amylase activity in children with high caries and no caries. Materials and Met...

  7. Maillard Reaction of Pidan White as Inhibited by Chinese Black Tea Extract (Camellia sinensis) in the Pickling Solution

    OpenAIRE

    Ganesan, Palanivel; Benjakul, Soottawat; Baharin, Badlishah Sham

    2014-01-01

    Changes in Maillard reaction of pidan white were monitored with A294, fluorescence intensity, and browning intensity during pickling in the absence and presence of Chinese black tea extract (Camellia sinensis) at levels of 2% and 5% together with 0.2% ZnCl2 or 0.2% CaCl2 up to 3 wk, followed by ageing for another 3 wk. Browning intensity and A294 of pidan white increased with increasing pickling/ageing, while fluorescence intensity decreased during ageing (p

  8. Green tea polyphenol, (−)-epigallocatechin-3-gallate, induces toxicity in human skin cancer cells by targeting β-catenin signaling

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Tripti [Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Katiyar, Santosh K., E-mail: skatiyar@uab.edu [Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Birmingham Veterans Affairs Medical Center, Birmingham, AL 35233 (United States)

    2013-12-01

    The green tea polyphenol, (−)-epigallocatechin-3-gallate (EGCG), has been shown to have anti-carcinogenic effects in several skin tumor models, and efforts are continued to investigate the molecular targets responsible for its cytotoxic effects to cancer cells. Our recent observation that β-catenin is upregulated in skin tumors suggested the possibility that the anti-skin carcinogenic effects of EGCG are mediated, at least in part, through its effects on β-catenin signaling. We have found that treatment of the A431 and SCC13 human skin cancer cell lines with EGCG resulted in reduced cell viability and increased cell death and that these cytotoxic effects were associated with inactivation of β-catenin signaling. Evidence of EGCG-induced inactivation of β-catenin included: (i) reduced accumulation of nuclear β-catenin; (ii) enhanced levels of casein kinase1α, reduced phosphorylation of glycogen synthase kinase-3β, and increased phosphorylation of β-catenin on critical serine{sup 45,33/37} residues; and (iii) reduced levels of matrix metalloproteinase (MMP)-2 and MMP-9, which are down-stream targets of β-catenin. Treatment of cells with prostaglandin E2 (PGE{sub 2}) enhanced the accumulation of β-catenin and enhanced β-catenin signaling. Treatment with either EGCG or an EP2 antagonist (AH6809) reduced the PGE{sub 2}-enhanced levels of cAMP, an upstream regulator of β-catenin. Inactivation of β-catenin by EGCG resulted in suppression of cell survival signaling proteins. siRNA knockdown of β-catenin in A431 and SCC13 cells reduced cell viability. Collectively, these data suggest that induction of cytotoxicity in skin cancer cells by EGCG is mediated by targeting of β-catenin signaling and that the β-catenin signaling is upregulated by inflammatory mediators. - Highlights: • EGCG inhibits cancer cell viability through inactivation of β-catenin signaling. • Inactivation of β-catenin involves the downregulation of inflammatory mediators. • EGCG

  9. Green tea polyphenol, (−)-epigallocatechin-3-gallate, induces toxicity in human skin cancer cells by targeting β-catenin signaling

    International Nuclear Information System (INIS)

    The green tea polyphenol, (−)-epigallocatechin-3-gallate (EGCG), has been shown to have anti-carcinogenic effects in several skin tumor models, and efforts are continued to investigate the molecular targets responsible for its cytotoxic effects to cancer cells. Our recent observation that β-catenin is upregulated in skin tumors suggested the possibility that the anti-skin carcinogenic effects of EGCG are mediated, at least in part, through its effects on β-catenin signaling. We have found that treatment of the A431 and SCC13 human skin cancer cell lines with EGCG resulted in reduced cell viability and increased cell death and that these cytotoxic effects were associated with inactivation of β-catenin signaling. Evidence of EGCG-induced inactivation of β-catenin included: (i) reduced accumulation of nuclear β-catenin; (ii) enhanced levels of casein kinase1α, reduced phosphorylation of glycogen synthase kinase-3β, and increased phosphorylation of β-catenin on critical serine45,33/37 residues; and (iii) reduced levels of matrix metalloproteinase (MMP)-2 and MMP-9, which are down-stream targets of β-catenin. Treatment of cells with prostaglandin E2 (PGE2) enhanced the accumulation of β-catenin and enhanced β-catenin signaling. Treatment with either EGCG or an EP2 antagonist (AH6809) reduced the PGE2-enhanced levels of cAMP, an upstream regulator of β-catenin. Inactivation of β-catenin by EGCG resulted in suppression of cell survival signaling proteins. siRNA knockdown of β-catenin in A431 and SCC13 cells reduced cell viability. Collectively, these data suggest that induction of cytotoxicity in skin cancer cells by EGCG is mediated by targeting of β-catenin signaling and that the β-catenin signaling is upregulated by inflammatory mediators. - Highlights: • EGCG inhibits cancer cell viability through inactivation of β-catenin signaling. • Inactivation of β-catenin involves the downregulation of inflammatory mediators. • EGCG inactivates

  10. Polyphenols and Glycemic Control.

    Science.gov (United States)

    Kim, Yoona; Keogh, Jennifer B; Clifton, Peter M

    2016-01-01

    Growing evidence from animal studies supports the anti-diabetic properties of some dietary polyphenols, suggesting that dietary polyphenols could be one dietary therapy for the prevention and management of Type 2 diabetes. This review aims to address the potential mechanisms of action of dietary polyphenols in the regulation of glucose homeostasis and insulin sensitivity based on in vitro and in vivo studies, and to provide a comprehensive overview of the anti-diabetic effects of commonly consumed dietary polyphenols including polyphenol-rich mixed diets, tea and coffee, chocolate and cocoa, cinnamon, grape, pomegranate, red wine, berries and olive oil, with a focus on human clinical trials. Dietary polyphenols may inhibit α-amylase and α-glucosidase, inhibit glucose absorption in the intestine by sodium-dependent glucose transporter 1 (SGLT1), stimulate insulin secretion and reduce hepatic glucose output. Polyphenols may also enhance insulin-dependent glucose uptake, activate 5' adenosine monophosphate-activated protein kinase (AMPK), modify the microbiome and have anti-inflammatory effects. However, human epidemiological and intervention studies have shown inconsistent results. Further intervention studies are essential to clarify the conflicting findings and confirm or refute the anti-diabetic effects of dietary polyphenols. PMID:26742071

  11. Polyphenols and Glycemic Control

    Directory of Open Access Journals (Sweden)

    Yoona Kim

    2016-01-01

    Full Text Available Growing evidence from animal studies supports the anti-diabetic properties of some dietary polyphenols, suggesting that dietary polyphenols could be one dietary therapy for the prevention and management of Type 2 diabetes. This review aims to address the potential mechanisms of action of dietary polyphenols in the regulation of glucose homeostasis and insulin sensitivity based on in vitro and in vivo studies, and to provide a comprehensive overview of the anti-diabetic effects of commonly consumed dietary polyphenols including polyphenol-rich mixed diets, tea and coffee, chocolate and cocoa, cinnamon, grape, pomegranate, red wine, berries and olive oil, with a focus on human clinical trials. Dietary polyphenols may inhibit α-amylase and α-glucosidase, inhibit glucose absorption in the intestine by sodium-dependent glucose transporter 1 (SGLT1, stimulate insulin secretion and reduce hepatic glucose output. Polyphenols may also enhance insulin-dependent glucose uptake, activate 5′ adenosine monophosphate-activated protein kinase (AMPK, modify the microbiome and have anti-inflammatory effects. However, human epidemiological and intervention studies have shown inconsistent results. Further intervention studies are essential to clarify the conflicting findings and confirm or refute the anti-diabetic effects of dietary polyphenols.

  12. Anti-inflammatory effects of a polyphenols-rich extract from tea (Camellia sinensis) flowers in acute and chronic mice models.

    Science.gov (United States)

    Chen, Bang-Tian; Li, Wei-Xi; He, Rong-Rong; Li, Yi-Fang; Tsoi, Bun; Zhai, Yu-Jia; Kurihara, Hiroshi

    2012-01-01

    While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis) flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE) could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected against Propionibacterium acnes (P. ances) plus lipopolysaccharide-(LPS-) induced liver inflammation by reversing the histologic damage and plasma alanine aminotransferase (ALT) increase. Moreover, the levels of nitric oxide (NO), tumor necrosis factor-(TNF)-α and interleukin-(IL-) 1β mRNA in mouse liver were markedly suppressed after treatment with TFE in mice with immunological liver inflammation. These results indicated that tea flowers had potent anti-inflammatory effects on acute and immunological inflammation in vivo, and may be used as a functional natural food. PMID:22900128

  13. Anti-Inflammatory Effects of a Polyphenols-Rich Extract from Tea (Camellia sinensis Flowers in Acute and Chronic Mice Models

    Directory of Open Access Journals (Sweden)

    Bang-Tian Chen

    2012-01-01

    Full Text Available While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected against Propionibacterium acnes (P. ances plus lipopolysaccharide-(LPS- induced liver inflammation by reversing the histologic damage and plasma alanine aminotransferase (ALT increase. Moreover, the levels of nitric oxide (NO, tumor necrosis factor-(TNF-α and interleukin-(IL- 1β mRNA in mouse liver were markedly suppressed after treatment with TFE in mice with immunological liver inflammation. These results indicated that tea flowers had potent anti-inflammatory effects on acute and immunological inflammation in vivo, and may be used as a functional natural food.

  14. 茶多酚对货架期泡菜气味成分的影响%The Effect of Tea Polyphenol on Volatile Flavor Compounds in Pickled Cabbage during Shelf Life

    Institute of Scientific and Technical Information of China (English)

    王向阳; 董欢欢; 俞兴伟

    2016-01-01

    Tea polyphenols is an excellent preservative for pickles.To find out the effects of tea polyphenols on the smell of pickle during shelf life,tea polyphenols (0.18%,W/V)is added into pickled cabbage.The change of volatile components of pickled cabbage is analyzed with solid phase micro-extraction and gas chromatography-mass spectrometry (SPME-GC/MS).The results show that tea polyphenols inhibit the growth of lactic acid bacteria,the main flavor compounds of pickled cabbage are ketones,acids,esters,aldehydes,alkenes and alcohols.The main odors components are 3-acetyl-4-hydroxy-6-methyl-2H-pyran-2-one,benzoic acids,allyl isothiocyanate,nonanal,caryophyllene and 1-octene-3-alcohol.The proportion of aldehydes and esters is increased significantly during pickles late shelf life.The benzene derivatives,alkene,alkyl,naphthalene are also increased considerably.The significant increasing of nonanal,decanal,allyl isothiocyanate,3-methyl-butyl butyrate might be the main reason for the late peculiar odors of pickled cabbage.Benzene derivatives,alkenes,naphthalene increasing might be the secondary reason for late odors.Acids and ketones in pickles are significantly reduced by tea polyphenol treatment and its ester and aldehydes reduce,but alcohols and alkenes increase at late shelf life ,and cause carbazole to disappear ,anthraquinone to increase,hydrazine to disappear,phenols to appear.Tea polyphenol treatment significantly reduces the 3-acetyl-4-hydroxy-6-methyl-2H-pyran-2-one,benzoic acid,isothiocyanates,nonanal,and significantly increases D-limo-nene at late shelf life.The main odor substances of tea polyphenol treatment pickle cabbage at late shelf life are D-limonene,3-acetyl-4-hydroxy-6-methyl-2H-pyran-2-one,1-octene-3-alcohol ketone.%茶多酚在腌菜中防腐效果好,为了解茶多酚对货架期泡菜气味的影响,在泡菜液中添加0.18%茶多酚,采用固相微萃取与气相质谱色谱联用法(SPME-MS/GC),分析泡菜

  15. Fluoride and aluminum in teas and tea-based beverages

    Directory of Open Access Journals (Sweden)

    Hayacibara Mitsue Fujimaki

    2004-01-01

    Full Text Available OBJECTIVE: To evaluate fluoride and aluminum concentration in herbal, black, ready-to-drink, and imported teas available in Brazil considering the risks fluoride and aluminum pose to oral and general health, respectively. METHODS: One-hundred and seventy-seven samples of herbal and black tea, 11 types of imported tea and 21 samples of ready-to-drink tea were divided into four groups: I-herbal tea; II-Brazilian black tea (Camellia sinensis; III-imported tea (Camellia sinensis; IV-ready-to-drink tea-based beverages. Fluoride and aluminum were analyzed using ion-selective electrode and atomic absorption, respectively. RESULTS: Fluoride and aluminum levels in herbal teas were very low, but high amounts were found in black and ready-to-drink teas. Aluminum found in all samples analyzed can be considered safe to general health. However, considering 0.07 mg F/kg/day as the upper limit of fluoride intake with regard to undesirable dental fluorosis, some teas exceed the daily intake limit for children. CONCLUSIONS: Brazilian and imported teas made from Camellia sinensis as well as some tea-based beverages are sources of significant amounts of fluoride, and their intake may increase the risk of developing dental fluorosis.

  16. Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats.

    Science.gov (United States)

    Wang, Haidong; Li, Deyuan; Hu, Zhongze; Zhao, Siming; Zheng, Zhejun; Li, Wei

    2016-06-30

    To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor-α (TNF-α), interleukin-1-β (IL-1-β) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb induced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols. PMID:27239812

  17. Bioactive compounds and antioxidant capacity of yellow Yinzhen tea affected by different extraction conditions

    OpenAIRE

    Mihelj, Tea; Belščak-Cvitanović, Ana; Komes, Draženka; Horžić, Dunja; Tomašić, Vlasta

    2014-01-01

    Tea is one of the most popular beverages consumed worldwide. Polyphenols in tea contribute to its health benefits possessing high antioxidant, anticarcinogenic and antiallergic activity. The aim of this study was to examine the effect of ethanol concentration, tea forms and storage duration on polyphenols and methylxanthines composition, as well as antioxidant capacity of yellow Yinzhen tea. Total polyphenol, flavonoid concentration and antioxidant capacity were measured spectrofotometrically...

  18. Green tea for the prevention of cancer: evidence of field epidemiology

    Directory of Open Access Journals (Sweden)

    Min Zhang

    2012-10-01

    Full Text Available Background: Tea is derived from the leaf of Camellia sinensis, a natural beverage widely consumed around the world. Geological and botanical evidence suggests that the tea plant originated from China. Varying methods of processing tea leaves lead to green tea, black tea, or Oolong tea, which differ in their concentrations of polyphenols. Green tea polyphenols appear to have anti-tumorigenic properties, and form 30-40% of the dry weight of green tea compared with only 3-10% of black tea. Numerous studies in multiple animal models and different cancer cell lines have demonstrated the anti-tumorigenesis by green tea polyphenols. Despite the consistency of laboratory results, evidence of this effect occuring in humans has been inconclusive to date.Objective: To investigate if green tea consumption was associated with longer survival rates in ovarian cancer patients, and a lower risk of ovarian, breast, and colorectal cancer, in addition toadult leukemiaMethods: We have conducted one prospective cohort study in ovarian cancer patients, and fivecase-control studies in ovarian, breast, and colorectal cancers, and leukemia over the past decade. Tea consumption was measured using a structured questionnaire by face-to-face interviews. The validity and reliability of the questionnaire was assessed in a preliminary study, and then evaluated by a test–retest. Cox proportional hazards regression models were used to obtain hazard ratios(HRs, 95% confidence intervals(95% CIs, and were adjusted for age at diagnosis, locality, body mass index(BMI, parity, International Federation of Gynecology and Obstetrics(IFGO stage, histologic grade of differentiation, cytology of ascites, residual tumour, and chemotherapeutic status. Odds ratios(ORs and 95% CIs were obtained using logistic regression analyses, which accounted for demographic, lifestyle, hormonal and family cancer factors, and potential confounders.Functional Foods in Health and Disease 2012, 2

  19. Impact of Magnesium Sulphate on Biochemical and Quality Constituents of Black Tea

    Directory of Open Access Journals (Sweden)

    S. Jayaganesh

    2010-01-01

    Full Text Available Field experiment was laid out in Randomized Block Design (RBD at UPASI tea experimental farm, with eight treatments and three replications. Amino acid content in tea leaves increased due to soil application of magnesium, but the 50% potassium reduction fields are reduced the amino acid content. The amino acid content significantly increased the soil applied magnesium when compared to the standard practices. The blocks which received the foliar application of micronutrient along with magnesium decreased the amino acid content when compared to the standard practices and control. The externally added magnesium increased the formation of 2-oxoglutarate and simultaneously another product aspartate/alanine was increased. The catechins content increased in 300 kg soil applied magnesium treatment and then decreased in 50% reduction of potassium fertilizer treatments. The TF value of made tea increased in 300 kg magnesium application plots and but decreased in the case of 50% reduction of potassium fertilizer. The control blocks having lower TF value when compared to the standard practices, because the K fertilizer is the important to increase the TF value. The maximum quantity of TF value obtained in the case of 300 kg soil applied blocks. The similar kind of trend was observed in TR values. Flavour index was made tea was higher in magnesium treated blocks, when compared to the control and NPK application. The magnesium content of tea leaves positively correlated with amino acid and amino acid transferase enzymes. This study further confirmed that antagonism exiting between K and Mg and synergism existing between P and Mg.

  20. Comparative effects of irradiation, fumigation, and storage on the free amino acids and sugar contents of green, black and oolong teas

    International Nuclear Information System (INIS)

    Food irradiation or chemical fumigation can be used to ensure the hygienic quality of teas. The comparative effects of gamma irradiation (5 and 10 kGy) and fumigation (MeBr and PH3) were investigated on the amino acids and sugar contents of Camellia sinensis (green, black and oolong teas) during storage (15±12 °C). The major amino acids found in teas were theanine and glutamic acid. Irradiation increased amino acids such as, leucine, alanine, and glutamic acid, and decreased the histidine. PH3 fumigation resulted in a decrease of tyrosine content; however, the effect of MeBr fumigation was negligible. Storage showed no significant effect on the amino acid content of the irradiated and fumigated teas. Sucrose, glucose, and fructose contents significantly increased upon gamma irradiation (p≤0.05). However, fumigation and subsequent storage did not affect the sugar contents. Irradiation could be a preferred alternative choice to address food safety problems as fumigation is restricted in many countries. - Highlights: ► Teas were investigated for the changes during storage on irradiation and fumigation. ► The effect on amino acid contents was more prominent on irradiation than fumigation. ► The sucrose, glucose, and fructose contents increased on gamma irradiation. ► Fumigation and storage did not affect the sugar contents in the teas. ► Irradiation could be an effective alternative for fumigants

  1. Protection against ultraviolet-B radiation-induced local and systemic suppression of contact hypersensitivity and edema responses in C3H/HeN mice by green tea polyphenols

    International Nuclear Information System (INIS)

    Exposure of skin to UV radiation can cause diverse biological effects, including induction of inflammation, alteration in cutaneous immune cells and impairment of contact hypersensitivity (CHS) responses. Our laboratory has demonstrated that oral feeding as well as topical application of a polyphenolic fraction isolated from green tea (GTP) affords protection against the carcinogenic effects of UVB (280-320 nm) radiation. In this study, we investigated whether GTP could protect against UVB-induced immunosuppression and cutaneous inflammatory responses in C3H mice. Immunosuppression was assessed by contact sensitization with 2,4-dinitrofluorobenzene applied to UVB-irradiated skin (local suppression) or to a distant site (systemic suppression), while double skin-fold swelling was used as the measure of UVB-induced inflammation. (author)

  2. Oligomerization and hydroxylation of green tea catechins by oxidative enzymes

    NARCIS (Netherlands)

    Verloop, J.W.

    2016-01-01

    Black teas are known for their characteristic brown colour, bitter taste and astringent mouth feel. These sensory characteristics are mainly influenced by the phenolic oxidation products present in black tea. The oxidation of phenolics from green tea leaves during black tea manufacturing is an uncon

  3. [Polycyclic aromatic hydrocarbons in tea and tea infusions].

    Science.gov (United States)

    Ciemniak, Artur; Mocek, Kamila

    2010-01-01

    Tea is the one of most widely consumed beverage in the world. It is generally believed that tea consumption might have health promoting properties. But residues of certain chemical compounds might impose a health threat on tea drinkers. The main contaminants are heavy metals, fluoride, pesticides and even dioxins. Tea lives which possess a high surface area can be contaminated with atmospheric PAHs. The manufacturing processes may also introduce PAHs into tea lives. The aim of his study was to determine the contamination of black, green, red and white teas by PAHs. In this investigation, content of 23 PAH, i.e 16 EPA PAH and 15 EU PAH were determined in 18 brands of tea and its infusions. The analytical procedure was based on ultrasonic extraction for dried tea and liquid-liquid extraction for infusions. All samples were cleaned up by florisil cartridge. The total content of 23 PAH varied between 22.9 microg/kg to 2945.5 microg/kg and 2.7 microg/kg to 63,1 microg/kg microg/kg for BaP. The analysed tea samples showed an increasing presence of PAH in the following order (mean value): black tea < red tea < green tea < white tea. However the highest content of PAH was found in the one brand of black tea bag both in sum of PAH and BaP content. During tea infusion 1.6% of total PAHs contained in tea was released into the beverage. The dominant PAHs in tea infusion were 2, 3 and 4 rings PAH, while the most toxic compounds were found at trace amounts. The concentrations of total 23 PAHs and BaP in tea infusions ranged from 332.5 ng/dm3 to 2245.9 ng/dm3 and 0.35 ng/dm3 to 18.7 ng/dm3 respectively. PMID:21365858

  4. Treatment of green tea polyphenols in hydrophilic cream prevents UVB-induced oxidation of lipids and proteins, depletion of antioxidant enzymes and phosphorylation of MAPK proteins in SKH-1 hairless mouse skin.

    Science.gov (United States)

    Vayalil, Praveen K; Elmets, Craig A; Katiyar, Santosh K

    2003-05-01

    The use of botanical supplements has received immense interest in recent years to protect human skin from adverse biological effects of solar ultraviolet (UV) radiation. The polyphenols from green tea are one of them and have been shown to prevent photocarcinogenesis in animal models but their mechanism of photoprotection is not well understood. To determine the mechanism of photoprotection in in vivo mouse model, topical treatment of polyphenols from green tea (GTP) or its most chemopreventive constituent (-)-epigallocatechin-3-gallate (EGCG) (1 mg/cm(2) skin area) in hydrophilic ointment USP before single (180 mJ/cm(2)) or multiple UVB exposures (180 mJ/cm(2), daily for 10 days) resulted in significant prevention of UVB-induced depletion of antioxidant enzymes such as glutathione peroxidase (78-100%, P 0.001). Further, to delineate the inhibition of UVB-induced oxidative stress with cell signaling pathways, treatment of EGCG to mouse skin resulted in marked inhibition of a single UVB irradiation-induced phosphorylation of ERK1/2 (16-95%), JNK (46-100%) and p38 (100%) proteins of MAPK family in a time-dependent manner. Identical photoprotective effects of EGCG or GTP were also observed against multiple UVB irradiation-induced phosphorylation of the proteins of MAPK family in vivo mouse skin. Photoprotective efficacy of GTP given in drinking water (d.w.) (0.2%, w/v) was also determined and compared with that of topical treatment of EGCG and GTP. Treatment of GTP in d.w. also significantly prevented single or multiple UVB irradiation-induced depletion of antioxidant enzymes (44-61%, P the possibility of its use for the humans, and the data obtained from this in vivo study further suggest that GTP could be useful in attenuation of solar UVB light-induced oxidative stress-mediated and MAPK-caused skin disorders in humans. PMID:12771038

  5. Ameliorating effect of black tea extract on cadmium chloride-induced alteration of serum lipid profile and liver histopathology in rats.

    Science.gov (United States)

    Mantur, Venkappa S; Somannavarib, Manjunath S; Yendigeri, Saeed; Das, Kusal K; Goudar, Shivaprasad S

    2014-01-01

    Cadmium is one among the most environmental pollutants that affects many organs like kidney, liver and testis. The present study was aimed to assess the simultaneous effects of black tea extracts (BTE) on cadmium chloride induced alterations in lipid profile and liver histology. Adult rats were divided into four groups (n=6/group), group I (normal saline), group II (CdCl2, 1.0 mg/kg, b.wt; i.p), group III (black tea extract, 2.5 gm tea leaf/dl of water that is 2.5% of aqueous BTE) and group IV (cadmium chloride + BTE). Cadmium chloride intoxicated rats showed significant increase in serum total cholesterol, triglycerides, and low density lipoprotein-cholesterol and there is a significant decrease in the serum high density lipoprotein-cholesterol. In the liver, cadmium chloride showed changes in normal architecture, swollen hepatocytes, kupffer cells hyperplasia, dilation and congestion of central vein. Oral administration of black tea extracts with cadmium chloride significantly improves lipid profile and liver architecture as compared to the cadmium chloride group. The results indicate that BTE is beneficial in preventing cadmium-induced lipid alterations and hepatocellular damage. PMID:25509961

  6. In vitro pancreatic lipase, cholesterol esterase and cholesterol micellization inhibitory activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.)

    Institute of Scientific and Technical Information of China (English)

    Wanigasekera Daya Ratnasooriya

    2015-01-01

    Objective:To access the pancreatic lipase, cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds ofCamellia sinensis L. Methods: Black tea brew (BTB) was made according to International Organization for Standardization 3103 specifications and concentrations ofBTB tested were 37.5, 75.0, 150.0, 300.0 and 600.0µg/mL for antilipase and anti-cholesterol esterase assays and 0.25, 0.50 and 1.00µg/mL for cholesterol micellization inhibitory assay. Results:The results showed thatBTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent (r2 = 0.95) cholesterol micellization inhibitory activityin vitro comparable to epigallocatechin gallate, the reference drug used. In contrast, BTB had only mild but dose-dependent (r2= 0.94) inhibitory activity against pancreatic lipase and weak inhibitory effect (up to 13.17%) on pancreatic cholesterol esterase. Conclusions: It is concluded that consumption ofBTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.

  7. In vitro pancreatic lipase, cholesterol esterase and cholesterol micellization inhibitory activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.

    Directory of Open Access Journals (Sweden)

    Wanigasekera Daya Ratnasooriya

    2015-07-01

    Full Text Available Objective: To access the pancreatic lipase, cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds of Camellia sinensis L. Methods: Black tea brew (BTB was made according to International Organization for Standardization 3103 specifications and concentrations of BTB tested were 37.5, 75.0, 150.0, 300.0 and 600.0 µg/mL for antilipase and anti-cholesterol esterase assays and 0.25, 0.50 and 1.00 µg/mL for cholesterol micellization inhibitory assay. Results: The results showed that BTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent (r 2 = 0.95 cholesterol micellization inhibitory activity in vitro comparable to epigallocatechin gallate, the reference drug used. In contrast, BTB had only mild but dose-dependent (r 2 = 0.94 inhibitory activity against pancreatic lipase and weak inhibitory effect (up to 13.17% on pancreatic cholesterol esterase. Conclusions: It is concluded that consumption of BTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.

  8. Polyphenol- and fibre-rich dried fruits with green tea attenuate starch-derived postprandial blood glucose and insulin: a randomised, controlled, single-blind, cross-over intervention.

    Science.gov (United States)

    Nyambe-Silavwe, H; Williamson, G

    2016-08-01

    Polyphenol- and fibre-rich foods (PFRF) have the potential to affect postprandial glycaemic responses by reducing glucose absorption, and thus decreasing the glycaemic response of foods when consumed together. A randomised, single-blind, cross-over study was conducted on sixteen healthy volunteers to test whether PFRF could attenuate postprandial blood glucose in healthy volunteers when added to a source of carbohydrate (starch in bread). This is the first study to examine the effects of a meal comprised of components to inhibit each stage of the biochemical pathway, leading up to the appearance of glucose in the blood. The volunteers were fasted and attended four visits: two control visits (bread, water, balancing sugars) and two test visits (single and double dose of PFRF) where they consumed bread, water and PFRF. Blood samples were collected at 0 (fasted), 15, 30, 45, 60, 90, 120, 150 and 180 min after consumption. The PFRF components were tested for α-amylase and α-glucosidase inhibitory potential in vitro. Plasma glucose was lower after consumption of both doses compared with controls: lower dose, change in mean incremental areas under the glucose curves (IAUC)=-27·4 (sd 7·5) %, Pamylase (green tea, strawberry, blackberry and blackcurrant) and α-glucosidase (green tea) activities in vitro. The PFRF have a pronounced and significant lowering effect on postprandial blood glucose and insulin response in humans, due in part to inhibition of α-amylase and α-glucosidase, as well as glucose transport. PMID:27278405

  9. Polyphenols as inhibitors of carcinogenesis.

    OpenAIRE

    Yang, C S; Lee, M. J.; Chen, L.; Yang, G.Y.

    1997-01-01

    Many polyphenolic compounds have demonstrated anticarcinogenic activities in animal models. These compounds include flavanone, flavonols, isoflavone, and catechins. In this article, tea catechins will be used as an example to illustrate current research in this area. Many laboratory studies have demonstrated the inhibition of tumorigenesis in animal models by different tea preparations. The animal models include tumorigenesis in the mouse lung, rat and mouse esophagi, mouse forestomach, mouse...

  10. Novel insights of dietary polyphenols and obesity

    OpenAIRE

    Wang, Shu; Moustaid-Moussa, Naima; Chen, Lixia; Mo, Huanbiao; Shastri, Anuradha; Su, Rui; Bapat, Priyanka; Kwun, InSook; Shen, Chwan-Li

    2014-01-01

    Prevalence of obesity has steadily increased over the past three decades both in the United States and worldwide. Recent studies have shown the role of dietary polyphenols in the prevention of obesity and obesity-related chronic diseases. Here we evaluated the impact of commonly consumed polyphenols, including green tea catechins and epigallocatechin gallates, resveratrol, and curcumin, on obesity and obesity-related-inflammation. Cellular studies demonstrated that these dietary polyphenols r...

  11. Effects of season and plantation on phenolic content of unfermented and fermented Sri Lankan tea.

    Science.gov (United States)

    Jayasekera, Shiromani; Kaur, Lovedeep; Molan, Abdul-Lateef; Garg, Manohar L; Moughan, Paul J

    2014-01-01

    The effects of season and plantation on the polyphenol content of Camellia sinensis (tea) leaves were determined. Aqueous and organic extracts of freeze-dried fresh (unfermented) and black (fully-fermented) tea leaves were prepared for a structured set of samples (fermented and unfermented teas from six high-, mid- and low-grown plantations; fermented and unfermented teas from two harvesting seasons from four highland plantations), collected from the main tea-growing regions in Sri Lanka. Total catechin content and amounts of individual catechins, flavonols and theaflavins were determined by HPLC. Mean values for the phenolic constituents were generally significantly higher (pextraction. The mean values for total catechins, total flavonols and caffeine in the aqueous extracts from unfermented teas were 10.6%, 1.5% and 2.9%, respectively. For both unfermented and fermented tea leaves, a significant (p<0.05) interaction between plantation and season was observed for phenolic constituents. Ferric reducing antioxidant power was positively (p<0.05) correlated with (-)-epicatechin gallate and total phenolic contents. PMID:24444973

  12. Dietary polyphenol intake in Europe

    DEFF Research Database (Denmark)

    Zamora-Ros, Raul; Knaze, Viktoria; Rothwell, Joseph A;

    2016-01-01

    BACKGROUND/OBJECTIVES: Polyphenols are plant secondary metabolites with a large variability in their chemical structure and dietary occurrence that have been associated with some protective effects against several chronic diseases. To date, limited data exist on intake of polyphenols in populations......-conscious group, followed by non-Mediterranean (non-MED) and MED countries. The main polyphenol contributors were phenolic acids (52.5-56.9 %), except in men from MED countries and in the UK health-conscious group where they were flavonoids (49.1-61.7 %). Coffee, tea, and fruits were the most important food...

  13. Allergic contact dermatitis to substitute hair dyes in a patient allergic to para-phenylenediamine: Pure henna, black tea and indigo powder.

    Science.gov (United States)

    Swan, Bonnie C; Tam, Mei M; Higgins, Claire L; Nixon, Rosemary L

    2016-08-01

    We report a case of a 50-year-old lady with allergic contact dermatitis to para-phenylenediamine, who in her quest to find a substitute hair dye, subsequently reacted to a number of plant-based hair dyes, including pure henna, black tea and indigo powder respectively. While these substances all contain tannins, testing to possible constituents tannic acid and gallic acid was negative. PMID:26916211

  14. Impact of flavonoid-rich black tea and beetroot juice on postprandial peripheral vascular resistance and glucose homeostasis in obese, insulin-resistant men: a randomized controlled trial

    OpenAIRE

    Fuchs, Dagmar; Nyakayiru, Jean; Draijer, Richard; Mulder, Theo P. J.; Hopman, Maria T. E.; Eijsvogels, Thijs M. H.; Thijssen, Dick H

    2016-01-01

    Background Insulin-stimulated muscle blood flow facilitates plasma glucose disposal after a meal, a mechanism that is impaired in obese, insulin-resistant volunteers. Nitrate- or flavonoid-rich products, through their proposed effects on nitric oxide, may improve postprandial blood flow and, subsequently, glucose disposal. To investigate whether a single dose of nitrate-rich beetroot juice or flavonoid-rich black tea lowers postprandial muscle vascular resistance in obese volunteers and alter...

  15. Differential Effects of Tea Extracts on Growth and Cytokine Production by Normal and Leukemic Human Leukocytes

    Directory of Open Access Journals (Sweden)

    Diana Bayer

    2012-04-01

    Full Text Available Background: Tea is one of the world’s most highly consumed beverages, second only to water. It is affordable and abundant and thus has great potential for improving health of those in both developed and developing areas. Green, oolong, and black teas differ in the extent of fermentation and types of bioactive polyphenols produced. Green tea and its major polyphenol decrease growth of some cancer cells and effect production of immune system cytokines. This study compares the effects of different types of tea extracts on viability and cytokine production by normal and leukemic human T lymphocytes. Generation of the toxic reactive oxygen species H2O2 by extracts was also examined.Methods: The Jurkat T lymphoblastic leukemia cells and mitogen-stimulated normal human peripheral blood mononuclear cells were used in this study. Cell viability was determined by (3-4,5-dimethylthiamizol-2-yl-diphenyltetrazolium bromide assay and production of interleukin-2 by Enzyme-Linked ImmunoSorbent Assay. Levels of H2O2 generated by tea extracts were determined using the xylenol-orange method.Results: We found that green, oolong, and black tea extracts differentially effect the growth and viability of T lymphoblastic leukemia cells and normal peripheral blood mononuclear cells, substantially decreasing both growth and viability of leukemic T lymphocytes and having much lesser effects on their normal counterparts. Tea extracts also had differential effects on the production of the T lymphocyte growth factor interleukin-2, significantly decreasing production by leukemic cells while having only minor effects on normal cells. All three extracts induced H2O2 generation, with green and oolong tea extracts having the greatest effect. Leukemic cells were much more susceptible to growth inhibition and killing by H2O2 than normal lymphocytes.Functional Foods in Health and Disease 2012, 2(4:72-85 Conclusions: The three tea extracts studied altered leukemic T lymphocyte

  16. The development of processing technology for black tea-salty sweet-biscuits%红茶咸香饼干加工工艺的研制

    Institute of Scientific and Technical Information of China (English)

    谢朝敏; 温立香; 王淋靓; 任二芳; 罗小杰; 艾静汶; 叶雪英

    2015-01-01

    研究红茶咸香饼干加工工艺。分别通过单因素实验确定了红茶的制备工艺和红茶的冲泡工艺,红茶制备采用鲜叶→萎凋(室温)→机械揉捻→轻发酵(35℃×3h,湿度90%)→烘干的制备工艺方案;红茶的冲泡采用洗茶→纯水仪过滤水冲泡(95℃×5min)→茶叶与茶汤分离→出茶的冲泡方案。利用正交实验确定了红茶咸香饼干的最佳配方为:低筋面粉180g、盐2g、泡打粉1.5g、黄油45g、红茶汤45g、全蛋液30g。%We researched the processing technology of black tea-salty sweet-biscuits.The preparation and brewing process of black tea were determined by single factor experiment and the preparation process program was that, fresh leaves→withering (room temperature)→mechanical rolling→light fermentation (35℃×3h,humidity 90%)→drying.The brewing process was that washing tea→brewed by filtered water of pure water meter(95℃×5min)→separating the tea and tea soup.And then,the best formula of black tea-salty sweet-biscuits was confirmed by an orthogonal experiment, the best technical skill was that low-gluten flour 180g, salt 2g, baking powder 1.5g, butter 45g,black tea soup 45g, whole egg 30g.

  17. Technological Study on Preparation of Low Caffeine Instant Black Tea with Fresh Tea Leaves%用鲜叶加工低咖啡因速溶红茶的工艺研究

    Institute of Scientific and Technical Information of China (English)

    高展; 刘伟; 龚正礼

    2015-01-01

    This article is oriented in studying the process technology of low caffeine instant black tea by or‐thogonal test with fresh tea leaves as raw material .By means of comprehensive assessment ,the most ap‐propriate proportion of added fresh tea leavesis 7∶3 ,i .e .decaffeinated fresh leaves per 70 g by adding 30 g chopped fresh leaves ,working as a source of enzyme fermentation for making out the best quality de‐caffeinated tea embryo .Four factors affecting theaflavins extraction from primary to secondary are stated as follows :pH ,temperature ,solid‐liquid ratio ,time .However ,the factors affecting thearubigins extrac‐tion from primary to secondary are solid‐liquid ratio ,pH ,temperature ,time .The optimal process parame‐ter for extraction of processing low caffeine instant black tea is at the temperature of 95℃ ,for the duration of 30 min extraction time ,with a solid‐liquid ratio 1∶15 and pH5 .5 .Through organoleptic evaluation ,it has been found that the better quality low caffeine instant black tea is made of fresh leaves and that the yield is also coming to 21 .4% .Besides ,there is not much difference in comparison of tradition process technique .T hus ,this process can be applied to the industrialized production of instant black tea .%以茶鲜叶为原料,采用正交试验,研究了低咖啡因速溶红茶的加工工艺.通过综合评估,鲜叶添加比例以7∶3最为合适,即每70 g脱咖啡因鲜叶中添加30 g切碎的鲜叶作为酶源发酵制成的脱咖啡因红茶胚品质最佳.4个因素对茶黄素浸提影响的主次关系为pH值,温度,固液比,时间,对茶红素浸提影响的主次关系则为固液比,时间,pH值,温度.鲜叶加工低咖啡因速溶红茶浸提的最优工艺参数为温度95℃,浸提时间30 min ,固液比1∶15, pH值为5.5.通过感官审评,发现用鲜叶制的低咖啡因速溶红茶品质更佳,且其得率也达到了21.4%,与传统加

  18. Effect of compound agent of celery powder, BLF and tea polyphenols on the quality of cured pork%芹菜粉、竹叶黄酮与茶多酚复配对腌肉品质的影响

    Institute of Scientific and Technical Information of China (English)

    石亚中; 伍亚华; 许晖; 钱时权; 徐家莉; 汪张贵

    2013-01-01

    用芹菜粉、竹叶黄酮和茶多酚复配,对腌肉制品的品质进行研究.以腌肉模糊感官综合评分和亚硝酸盐残留量的平均值作为主要评定指标,通过正交实验进行直观分析,优化腌肉复配剂的最佳配比,并探讨该复配剂对腌肉制品品质的影响.结果表明,影响腌肉品质因素的主次顺序为:芹菜粉>茶多酚>竹叶黄酮,优化组合为芹菜粉0.30%,茶多酚400mg/kg,竹叶黄酮200mg/kg,该条件下腌制咸肉,30d时腌肉的亚硝酸盐残留量为2.87mg/kg,TBARS和TVBN含量分别为0.39nmol/mg prot,10.05mg/100g,明显优于对照组,所得腌肉感官模糊评价得分为5.72,属于Ⅰ级,表明该复配剂能有效降低腌肉亚硝酸盐残留量,提高腌肉制品品质.%The quality of cured pork was researched with compound curing agent of celery powder, bamboo -leaf-flavonoids(BLF) and tea polyphenols. Taking the mean value of fuzzy comprehensive sensory score and the content of nitrite residue as the main evaluation index,determined the optimal ratio of the compound agent through the orthogonal experiment of visual analysis,and the effect of cured pork was dicussed with the optimal compound curing agent. The result showed that the optimal mix parameters were celery powder 0.30%,tea polyphenols 400mg/kg and BLF 200mg/kg. After cured 30d,the residual nitrite of the optimal compound curing agent group was 2.87mg/kg,fuzzy sensory score was 5.72,which was belonged to grade I . The TBARS and TVBN of cured pork was 0.39nmol/mg prot and 10.05mg/100g respectively .which were significantly lower than the control group,indicating the compound curing agent could reduce the residual nitrite of cured meat,improve the quality of cured pork effectively.

  19. Polyphenolics profile, antioxidant and radical scavenging activity of leaves and stem of Raphanus sativus L.

    Science.gov (United States)

    Beevi, Syed Sultan; Narasu, Mangamoori Lakshmi; Gowda, Bandi Boje

    2010-03-01

    Aerial parts (leaves and stem) of Raphanus sativus, which are usually discarded were found to possess potent antioxidant and radical scavenging activity, as measured by standard antioxidant assays. Methanolic and acetone extracts of R. sativus leaves had total polyphenolic content of 86.16 and 78.77 mg/g dry extract, which were comparable to the traditional rich sources such as green tea and black tea. HPLC identification of polyphenolics indicated the presence of catechin, protocatechuic acid, syringic acid, vanillic acid, ferulic acid, sinapic acid, o-coumaric acid, myricetin, and quercetin in leaves and stem. Among the different extraction solvents, methanolic extract of leaves and stem showed potent reductive capacity, significantly inhibited linoleic acid peroxidation and displayed metal chelating activity. Further, they scavenged free radicals effectively with IC50 (half maximal inhibitory concentration) of 31 and 42 microg/ml for DPPH radical, 23 and 52 microg/ml for superoxide radical, 67 and 197 microg/ml for hydrogen peroxide,and 56 and 62 microg/ml for nitric oxide, respectively. Leaves showed most potent antioxidant and radical scavenging activity as compared to stem, which may be accounted for the high polyphenolic content. Leaves and stem of R. sativus,often under-utilized part of this vegetable, thus possessed considerable amount of polyphenolics. Hence, it should be egarded as a potential source of natural antioxidants and could be effectively employed as an ingredient in health or in functional food. PMID:20072818

  20. Study on Reaction Kinetics Characteristics of Polyphenol Oxidase of Cryopreservation Fresh Tea Leaves%冻存茶鲜叶多酚氧化酶动力学特性研究

    Institute of Scientific and Technical Information of China (English)

    孔俊豪; 杨秀芳; 涂云飞; 孙庆磊; 陈小强

    2011-01-01

    The polyphenol oxidase activity of cryopreservation fresh tea leaves was determined by spectrophotometric method. The influence of main factors, such as substrate concentration, system pH and temperature on catalytic activities of PPO were investigated. The results showed that, the optimum pH and temperature for the enzymatic reaction was 4.6~5.0 and 35~60 ℃, respectively .The Michaelis-Menten equation of PPO from cryopreservation fresh tea leaves was established, accordingly,1/v=1.168 8/[S]+3.083 3, by which the kinetic parameters obtained were as follow: Km=0.378 mol/L,vmax=0.324 U /min.%采用分光光度法对冻存处理的茶树鲜叶的多酚氧化酶(PPO)进行了活性测定,研究了底物浓度、pH值及温度对冻存茶鲜叶PPO活性的影响,建立了茶树鲜叶冻存-萎凋过程的反应动力学方程.结果表明,冻存茶鲜叶PPO酶活的最适条件为:pH值介于4.6~5.0之间,温度35~60℃.由Michelis-Menten方程1/v=1.1688/[S]+3.083 3,得到的动力学参数为:Km=0.378mol/L,vmax=0.324U/min.

  1. Research Advances on Preventive and Curative Effect of Tea Polyphenols on Skin Photoaging%茶多酚对皮肤光老化的防治作用研究进展

    Institute of Scientific and Technical Information of China (English)

    马蕊; 林勇; 陈金华; 刘仲华

    2012-01-01

    皮肤光老化是由于长期紫外线辐射导致皮肤加速衰老的现象,光老化可以导致严重的后果,约80%以上的暴露部位美容问题由光老化引起,严重影响人们的外貌美观,并可能引发各种良性或恶性肿瘤。因此预防和延缓皮肤衰老受到人们广泛的关注。茶多酚是从天然植物茶叶中分离提取出来的多酚类化合物的复合体,是一种理想的天然抗氧化剂,具有延缓衰老和抵抗紫外线辐射等多种功效。%Photoaging is the lead to serious consequences primary factor of skin-aging owing to long-term ultraviolet UV radiation exposure. It can that about 80 % skin damages happen which seriously affects the appearance of people. And photoaging is closely related to a variety of skin diseases and skin cancer. Therefore, prevention and treatment of skin-aging is a hotly debated topic. Tea polyphenols is extracted from tea plant. It is a kind of ideal natural antioxidants, which can alleviate skin-aging and resist UV radiation.

  2. Aged leaves effect on essential components in green and oolong tea

    OpenAIRE

    Rahman, M.M.

    2013-01-01

    Tea leaf encompasses essential components like caffeine, polyphenol, lipid etc. The study was conducted to find out the essential constituents in green and oolong tea from aged tea leaves during the year of 2012. Tea leaves with different plucked aged were collected from Lackatoorah Tea Estate at sylhet district in Bangladesh. Collected leaves were processesed to make desirable samples for biochemical analysis in the laboratory of Department of Food Enginnering and Tea Technology, SUST. Resul...

  3. DFT/TDDFT and Experimental Studies of Natural Pigments Extracted from Black Tea Waste for DSSC Application

    Directory of Open Access Journals (Sweden)

    N. T. R. N. Kumara

    2013-01-01

    Full Text Available We report results of combined experimental and theoretical studies of black tea waste extract (BTE as a potential sensitizer for TiO2-dye-sensitized solar cells (DSSCs. UV-vis absorption data revealed that BTE contains theaflavin. DSSC sensitized with pigment complexes of BTE showed a photon-energy conversion efficiency of %, while a significant increase (% is observed when pH of the pigment solution was lowered. The HOMO and LUMO energy levels were calculated using experimental data of UV-vis absorption and cyclic voltammetry. These calculations revealed a reduction of the band gap by 0.17 eV and more negativity of HOMO level of acidified pigment, compared to that of original pigment. Combined effect of these developments caused the enhanced efficiency of DSSC. Density functional theory (DFT and time-dependent density functional theory (TDDFT computational calculations were carried out to study the four theaflavin analogues which are responsible for the dark colour of BTE. According to the calculations, two theaflavin analogues, theaflavin and theaflavin digallate, are the most probable sensitizers in this dye-sensitized solar cell system.

  4. Multielement analysis of tea leaves by ICP-OES and characterization using probabilistic neural networks

    International Nuclear Information System (INIS)

    Complete text of publication follows. Tea is one of the most popular and widely consumed beverages in the world with an estimated per capita worldwide consumption of 40 L of beverage per year (J.H. Weisburger, Cancer Lett., 114 (1997) 315-317). It is made from the leaves of the plant Camellia sinensis (L). Tea has important physiological properties and potential health benefits due to the presence of compounds such as polyphenols, amino acids, vitamins, carbohydrates, caffeine and purine alkaloids. It is also an important source of minerals. A regular consumption contributes to the daily dietary requirements of several elements. There are different varieties with different qualities due to morphologic and chemical diversities. Green and black are the most popular categories. Drying and roasting the leaves produce green tea, and with black tea, leaves are additionally fermented. If this fermentation is partially carried out, Oolong tea is obtained. Less consumed but also highly appreciated are white and Pu-erh teas. White tea is an unfermented tea made from new growth buds and young leaves of the plant. Pu-erh teas are mostly unfermented from a variety of C. sinensis found in the mountains of southern Yunnan (A. Alcazar, et al., J. Agric. Food Chem., 55 (2007) 5590-5596.). The metal content of the plants is related to the chemical composition of the soil and the irrigation water. The metal distribution depends on the different parts of the plant (S. Tokalioiglu, S. Kartal, Instr. Sci. Techn., 32 (2004) 387-400.) and, in this way, some differences could be expected between different varieties of tea. Also, differences in the mineral content can be associated to the elaboration process of the tea varieties. In this work the content on Al, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Sr, Zn in different types of tea has been determined by inductively coupled plasma atomic emission spectroscopy. By applying probabilistic neural networks (PNN) tea types have been

  5. Hydrolysis of green tea residue protein using proteolytic enzyme derived from Aspergillus oryzae

    OpenAIRE

    Xu, Yong-Quan; Zhong, Xiao-Yu; Chen, Su-Qin; Yin, Jun-Feng

    2011-01-01

    Free amino acids are important chemical components which impact the taste of green tea infusion. The hydrolysis of water-insoluble protein in the green tea residue helps to increase the contents of free amino acids components except theanine. Studies indicate that the hydrolysis of the tea protein could be restricted due to interaction of polyphenols with protein. The experiment indicates that the hydrolysis of tea protein by protease is the main trend when the polyphenols concentration is lo...

  6. Antimicrobial activities of tea catechins and theaflavins and tea extracts against Bacillus cereus

    Science.gov (United States)

    We evaluated the antimicrobial activities of seven green tea catechins and four black tea theaflavins, generally referred to as flavonoids, as well as the aqueous extracts (infusions) of 36 commercial black, green, oolong, white, and herbal teas against Bacillus cereus (strain RM3190) incubated at 2...

  7. Inhibition of salivary amylase by black tea in high-caries and low-caries index children: A comparative in vivo study

    Science.gov (United States)

    Arya, Vishal; Taneja, Lavina

    2015-01-01

    Introduction: Dental caries is a universal disease. Dietary modification is important in reducing the occurrence of dental caries. Tea which is so frequently consumed with cariogenic starch rich food is proposed to have anticariogenic potential. The various mechanism has been proposed for same and one being inhibition of salivary amylase activity. Aim: To determine the effect of 1.5% black tea decoction on salivary amylase activity in children with high caries and no caries. Materials and Methods: A total of 30 children in the age group of 12–15 years were selected for the study. They were further grouped based on their decayed missing filled surface (DMFS) score into high-caries group (DMFS above 10) and no-caries group (DMFS = 0). After 2 h of fasting, subjects consumed two salted crackers for 60 s following which they rinsed with water and then with black tea decoction (1.5%) the very next day. Retained food particles were recovered salivary amylase activity was noted as maltose to sucrose ratio via chromatography. Results: The average ratio of maltose to sucrose ratio percentage reduction in high-caries group was 43.63% and 41.17% in no caries group which was highly significant (P children with high- and no-caries index. PMID:27313414

  8. Antioxidant potential of tea reduces arsenite induced oxidative stress in Swiss albino mice.

    Science.gov (United States)

    Sinha, D; Roy, S; Roy, M

    2010-04-01

    Environmental arsenic (As) is a potent human carcinogen and groundwater As contamination is a major health concern in West Bengal, India. Oxidative stress has been one of the prime factors in As-induced carcinogenicity. Generation of reactive oxygen species (ROS), beyond the body's endogenous antioxidant balance cause a severe imbalance of the cellular antioxidant defence mechanism. Tea, a popular beverage has excellent chemopreventive and antioxidant properties. In this study it was investigated whether these flavonoids could ameliorate the arsenite (As III) induced oxidative stress in Swiss albino mice. Bio-monitoring with comet assay elicited that the increase in genotoxicity caused by As III was counteracted by both black tea and green tea. Elevated levels of lipid peroxides and protein carbonyl by As III were effectively reduced with green as well as black tea. They also exhibited protective action against the As III induced depletion of antioxidants like catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST) and glutathione (GSH) in mice liver tissue. Thus the tea polyphenols by virtue of their antioxidant potential may be used as an effective agent to reduce the As III induced oxidative stress in Swiss albino mice. PMID:20096321

  9. 茶多酚、吡美莫司、他克莫司外用对莫诺苯宗诱导白癜风样模型小鼠的疗效比较%Comparison of topical application of tea polyphenol versus pimecrolimus versus tacrolimus for the treatment of monobenzone-induced vitiligo-like depigmentation in a mouse model

    Institute of Scientific and Technical Information of China (English)

    李情; 祝逸平; 许爱娥

    2015-01-01

    目的 探讨茶多酚、吡美莫司、他克莫司对莫诺苯宗诱导的白癜风样模型小鼠的疗效差异.方法 45%莫诺苯宗诱导C57BL/6三周龄雌小鼠脱色,建立白癜风样动物模型.并研究他克莫司、吡美莫司、茶多酚对白癜风样模型小鼠脱色的治疗效果.通过肉眼观察毛发脱色及脱色面积,实验结束后取非用药部位脱色皮肤行组织学检查,HE染色检测淋巴细胞浸润情况,共聚焦激光扫描显微镜(RCM)观察小鼠皮肤的黑素和黑素细胞,免疫荧光检测CD8+T细胞.结果 模型组小鼠在用药部位及非用药部位均有脱色现象.他克莫司组、吡美莫司组、茶多酚组小鼠脱色减少,出现时间晚和面积指数均较模型组低;且用药部位脱色斑局部淋巴细胞和CD8+T细胞浸润减少.其中他克莫司组疗效优于其他组.结论 他克莫司、吡美莫司、茶多酚对白癜风样模型小鼠均有疗效.%Objective To compare the therapeutic effect of topical application of tea polyphenol versus pimecrolimus versus tacrolimus for monobenzone-induced vitiligo-like depigmentation in mice.Methods Twentyfive 3-week-old female C57BL/6 mice were randomly and equally divided into 5 groups:negative control group,model group,tea polyphenol group,pimecrolimus group,tacrolimus group.Monobenzone 45% cream was applied to the back of mice in all the five groups except the negative control group once daily for 40 consecutive days to establish a model of vitiligo-like depigmentation.During the induction of depigmentation,the tea polyphenol group,pimecrolimus group and tacrolimus group were topically treated with tea polyphenol,pimecrolimus and tacrolimus respectively,and the model group remaining untreated.The depigmentation of hairs and skin was observed by naked eyes on a daily basis.Tissue specimens were obtained for histological examination from depigmented skin at nonapplication sites in mice after the end of the experiment

  10. The green tea polyphenol EGCG inhibits E. coli biofilm formation by impairing amyloid curli fibre assembly and downregulating the biofilm regulator CsgD via the σ(E) -dependent sRNA RybB.

    Science.gov (United States)

    Serra, Diego O; Mika, Franziska; Richter, Anja M; Hengge, Regine

    2016-07-01

    In bacterial biofilms, which are often involved in chronic infections, cells are surrounded by a self-produced extracellular matrix that contains amyloid fibres, exopolysaccharides and other biopolymers. The matrix contributes to the pronounced resistance of biofilms against antibiotics and host immune systems. Being highly inflammatory, matrix amyloids such as curli fibres of Escherichia coli can also play a role in pathogenicity. Using macrocolony biofilms of commensal and pathogenic E. coli as a model system, we demonstrate here that the green tea polyphenol epigallocatachin gallate (EGCG) is a potent antibiofilm agent. EGCG virtually eliminates the biofilm matrix by directly interfering with the assembly of curli subunits into amyloid fibres, and by triggering the σ(E) cell envelope stress response and thereby reducing the expression of CsgD - a crucial activator of curli and cellulose biosynthesis - due to csgD mRNA targeting by the σ(E) -dependent sRNA RybB. These findings highlight EGCG as a potential adjuvant for antibiotic therapy of biofilm-associated infections. Moreover, EGCG may support therapies against pathogenic E. coli that produce inflammatory curli fibres along with Shigatoxin. PMID:26992034

  11. Incubator proof miniaturized Holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections

    Science.gov (United States)

    Peter, Beatrix; Nador, Judit; Juhasz, Krisztina; Dobos, Agnes; Körösi, Laszlo; Székács, Inna; Patko, Daniel; Horvath, Robert

    2015-06-01

    The in situ observation of cell movements and morphological parameters over longer periods of time under physiological conditions is critical in basic cell research and biomedical applications. The quantitative phase-contrast microscope applied in this study has a remarkably small size, therefore it can be placed directly into a humidified incubator. Here, we report on the successful application of this M4 Holomonitor to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications. A special mechanical stage was developed to position the sample into that range of the optical arrangement where digital autofocusing works with high reproducibility and precision. By in-depth analyzing the obtained single cell morphological parameters, we show that the limited vertical resolution of the optical setup results in underestimated single cell contact area and volume and overestimated single cell averaged thickness. We propose a simple model to correct the recorded data to obtain more precise single cell parameters. We compare the results with the kinetic data recorded by a surface sensitive optical biosensor, optical waveguide lightmode spectroscopy.

  12. In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea(Camellia sinensis L.)

    Institute of Scientific and Technical Information of China (English)

    Wanigasekera; Daya; Ratnasooriya; Walimuni; Prabhashini; Kaushalya; Mendis; Abeysekera; Chatura; Tissa; Dayendra; Ratnasooriya

    2014-01-01

    Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500,1.000 and 2.000 mg/mL) of black tea brew(BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate(EGCG) was used as the reference agent(concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL).Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid(from rooster comb)and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC50=(1.09±0.12) mg/mL]and dose dependent(r2=0.94) anti-hyaluronidase activity.EGCG also exhibited dose dependent(r2=0.93,P<0.05) anti-hyaluronidase activity which was superior[IC50=(0.09±0.00) mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.

  13. Evaluation of the Stability of the Total Antioxidant Capacity, Polyphenol Contents, and Starch Hydrolase Inhibitory Activities of Kombucha Teas Using an In Vitro Model of Digestion

    Directory of Open Access Journals (Sweden)

    Mindani I. Watawana

    2015-01-01

    Full Text Available The objective of this study was to evaluate and compare antioxidant and starch hydrolase inhibitory activity of three different types of Kombucha beverages prepared by three pellicles with different microbial compositions. The fermentation process was carried out for 7 days and the assessments of antioxidant and starch hydrolase inhibitory activities as well as tea phenolic compounds were carried out. These parameters were also evaluated after subjecting the final fermented samples to gastric and duodenal digestion in an in vitro digestion model. The pH had a statistically significant decrease during the period of fermentation. The total phenolics content and antioxidant activities had increased during the fermentation process as well as when subjected to digestion. The starch hydrolase inhibitory activities also increased in a similar manner during the different phases. The α-amylase and α-glucosidase inhibitory activities showed statistically significant increases (P<0.05 as the fermentation progressed, while an increase was observed after being subjected to pancreatic and duodenal digestion as well. All three types of tea showed a higher α-amylase inhibitory activity than α-glucosidase inhibitory activity.

  14. The effects of green tea derived catechins upon adipocyte metabolism

    OpenAIRE

    Mehra, Anisha

    2010-01-01

    Tea, from the plant Camellia Sinensis, is after water, the most consumed drink in the world. Green tea, produced by steaming freshly harvested leaves to prevent fermentation, is high in polyphenols known as catechins or flavanols. The major flavanols found in green tea include (-) epigallocatechin-3-gallate (EGCG) and (-) epicatechin. Literature reports suggest that green tea flavanols have the potential to exert anti-obesity effects by modulating weight gain and other factors such as lipogen...

  15. Cancer prevention by green tea: evidence from epidemiologic studies1234

    OpenAIRE

    Yuan, Jian-Min

    2013-01-01

    In contrast to the consistent results of an inhibitory effect of green tea extracts and tea polyphenols on the development and growth of carcinogen-induced tumors in experimental animal models, results from human studies are mixed. Both observational and intervention studies have provided evidence in support of a protective role of green tea intake in the development of oral–digestive tract cancer or an inhibitory role of oral supplementation of green tea extract on a precancerous lesion of o...

  16. Green tea polyphenol epigallocatechin-3-gallate inhibits the expression of nitric oxide synthase and generation of nitric oxide induced by ultraviolet B in HaCaT cells

    Institute of Scientific and Technical Information of China (English)

    SONG Xiu-zu; BI Zhi-gang; XU Ai-e

    2006-01-01

    Background Nitic oxide (NO) has been implicated in the pathogenesis of various inflammatory diseases, including sunburn and pigmentation induced by ultraviolet irradiation. Epigallocatechin-3-gallate (EGCG) is the major effective component in green tea and can protect skin from ultraviolet-induced damage. The purpose of this study was to investigate the protective mechanisms of EGCG on inducible nitric oxide synthase (iNOS) expression and NO generation by ultraviolet B (UVB) irradiation in HaCaT cells.Methods HaCaT cells were irradiated with UVB 30 mJ/cm2 and pretreated with EGCG at varying concentrations. The iNOS mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and NO production was quantified by spectrophotometric method. The expression of NF-κB P65 was measured by immunofluorescence cytochemistry staining. Results The expression of iNOS mRNA and generation of NO in HaCaT cells were increased by UVB irradiation. EGCG down regulated the UVB-induced iNOS mRNA synthesis and NO generation in a dose dependent manner. The UVB-induced activation and translocation of NF-κB were also down regulated by EGCG treatment in HaCaT cells (P<0.01).Conclusions Green tea derived-EGCG can inhibit and down regulate the UVB-induced activation and translocation of NF-κB, expression of iNOS mRNA and generation of NO respectively, indicating EGCG may play a protective role from UVB-induced skin damage.

  17. The Application of Tea Dyeing to Silk

    Institute of Scientific and Technical Information of China (English)

    金成嬉

    2001-01-01

    Vegetable dyes are eco-friendly throughout the full production process. A study is conducted with the purpose of assessing the properties of dye extracted from green tea, black tea and the tea tree cultivated and used in Jiang Nan area of China. The extracted dyes are applied with and without mordants on silk fabric and the dyeing properties are evaluated.

  18. Epigallocathechin gallate, polyphenol present in green tea, inhibits stem-like characteristics and epithelial-mesenchymal transition in nasopharyngeal cancer cell lines

    Directory of Open Access Journals (Sweden)

    Lin Chien-Hung

    2012-10-01

    Full Text Available Abstract Background Previous studies have demonstrated that the consumption of green tea inhibits the growth of various cancers. Most cancers are believed to be initiated from and maintained by a small population of cancer stem-like cells (CSC or tumor-initiating cells (TIC that are responsible for tumor relapse and chemotherapeutic resistance. Although epigallocathechin gallate (EGCG, the most abundant catechin in green tea, has been reported to induce growth inhibition and apoptosis in some cancer cells, its effect on CSC is undefined. In this study, we enriched CSC by the sphere formation, and provided an efficient model for further experiments. Using this method, we examined the effects of EGCG regulating the nasopharyngeal carcinoma (NPC CSC and attempted to elucidate the possible mechanisms. Methods NPC TW01 and TW06 cell lines were enriched by sphere formation and characterized their phenotypical properties, such as invasion capacity, epithelial-mesenchymal transition (EMT and gene expression were analyzed by quantitative real-time reverse transcription polymerase chain reaction (q-RT-PCR. EGCG-induced growth inhibition in the parental and sphere-derived cells was determined by MTT and bromodeoxyuridine (BrdU assay. EGCG-induced apoptosis was analyzed by flow cytometry with Annexin V and PI staining. The effects of EGCG on sphere-derived cell tumorigenicity, migration and invasion were determined by soft agar assay, wound healing, and cell invasion assay. The alternation of protein expression regulated by EGCG on these sphere-derived cells was assessed by immunofluorescence staining and western blot. Results NPC sphere-derived cells grown in serum-free non-adherent culture showed increased expression of stem cell markers and EMT markers compared to parental cells grown in conventional culture. Although EGCG induced growth inhibition and apoptosis in the parental cells in a dose-dependent manner, it was not as effective against spheres

  19. Effect of Chemical Composition of Honey on Cream Formation in Honey Lemon Tea

    OpenAIRE

    J. Hategekimana; J.G. Ma, Y. Li, E. Karangwa, W. Tang and F. Zhong

    2011-01-01

    The aim of this study was to assess the effect of chemical composition of honey and environmental factors such as pH, storage temperature and ionic strength on cream formation in honey lemon tea drink. The results showed that minerals, Ca2+ and Mg2+ were involved in cream formation while Fe2+ participated in changing color to tar black at pH 4.12 and above. Honey rich in polyphenols and proteins increased the cream to 2.51%. High proline content (0.506 mg/g) induced creaming in GH (Germany Ho...

  20. 白毫早鲜叶茶多酚氧化酶酶学特性%Features of Polyphenol Oxidase in Baihaozao Fresh Tea Leaves

    Institute of Scientific and Technical Information of China (English)

    孙慕芳; 张洁; 郭桂义

    2014-01-01

    为了解白毫早鲜叶中的 PPO酶学特性,采用分光光度法,研究底物浓度、温度和 pH 对白毫早鲜叶 PPO 活性的影响以及 PPO 的热稳定性。结果表明:PPO 活性随着温度、pH 的增大呈先增大后减少的钟形曲线关系,其最适温度为40℃,最适 pH 为6.0;其耐热性较强,于80℃处理1min 可钝化酶活力。酶促反应在温度37℃、pH 5.6柠檬酸-磷酸缓冲液、邻苯二酚为底物条件下,Km值和 Vmax分别为0.6217 mol/L 和4.46 U/min。%In order to explore the enzymatic characteristics of PPO in Baihaozao fresh tea leaves, spectrophotometric method was employed to study the effects of substrate concentration,temperature and pH on PPO activity and its thermal stability.Results:PPO activity first increased and then decreased with temperature and pH increasing.The suitable temperature and pH was 40℃ and 6.0 respectively;The thermal stability was strong,and the enzyme activity could be passivated after treated at 80℃ for one minute.Values of Km and Vmax were respectively 0.6217 mol/L and 4.46 U/min under the following conditions:temperature 37℃,pH 5.6 and substrate of catechol.

  1. Evaluation of Aluminum in Iranian Consumed Tea

    OpenAIRE

    Alireza Asgari; Mahdi Ahmadi Moghaddam; Amirhossein Mahvi; Masoud Yonesian

    2008-01-01

    Introduction: Black tea leaf is one of the most important sources of Aluminum in dietary. Therefore this research was conducted to assess the amount of Aluminum in Iranian tea infusion. Methods: To assess Aluminum in Iranian consumed tea, 27 tea samples were analyzed for Al concentration for 10 and 60 min infusion, aluminum concentration was measured with atomic absorption and the results were analyzed by SPSS.13 version. Results: The results showed that minimum and maximum concentration of A...

  2. ESTIMATING BIOCHEMICAL PARAMETERS OF TEA (CAMELLIA SINENSIS (L.)) USING HYPERSPECTRAL TECHNIQUES

    OpenAIRE

    Bian, M.; Skidmore, A.K.; Schlerf, M.; Liu, Y; Wang, T.

    2012-01-01

    Tea (Camellia Sinensis (L.)) is an important economic crop and the market price of tea depends largely on its quality. This research aims to explore the potential of hyperspectral remote sensing on predicting the concentration of biochemical components, namely total tea polyphenols, as indicators of tea quality at canopy scale. Experiments were carried out for tea plants growing in the field and greenhouse. Partial least squares regression (PLSR), which has proven to be the one of th...

  3. Effects of Baking Time for Improving Aroma on the Quality of Congou Black Tea%烘焙提香时间对工夫红茶品质的影响

    Institute of Scientific and Technical Information of China (English)

    孙庆娜; 张丽霞; 王小会; 向勤锃; 黄晓琴

    2012-01-01

    为提高工夫红茶的甜香度,本文以山东夏季鲜叶加工的工夫红茶初制品为原料,在70℃的烘温下进行不同时间的烘焙提香处理,并通过感官审评、生化成分和香气分析方法研究其对工夫红茶品质的影响,结果表明:烘焙时间的长短不仅影响工夫红茶的香型和高低,而且也影响汤色和滋味,在本试验条件下,烘焙时间以1.0~1.5h的感官品质较好;此外,烘焙时间对工夫红茶各生化成分的影响不同,但对品质有利的生化成分以烘焙1.0h的茶样含量最高;随着烘焙时间的延长,工夫红茶香气的种类、含量以及组分比值发生显著变化,从而可以解释不同烘焙时间工夫红茶香型存在差异的原因。%In order to improve the sweet flavor of congou black tea,the primary congou black tea,which was made from the summer fresh tea leaves picked in Shandong province,had been baked at 70℃ for different time.The effect of baking time on their quality was estimated by sensory evaluation,biochemical compositions determination and aroma analysis.The results showed that the baking time could affect the types and concentration of aroma as well as the liquor color,the taste of congou black tea.The sensory quality of congou black tea which was baked for 1.0 ~ 1.5 h at the temperature of 70℃,was better.There was different effect of baking time on different types of biochemical compounds.Baking 1h resulted in the high content of biochemical compounds which were beneficial to the quality of congou black tea.As the baking time increased,the types,contents and ratio of aroma compounds of congou black tea changed,which could the reason why the different baking time made different aroma of congou black tea.

  4. 血管内皮细胞内皮素分泌功能与茶多酚及血管紧张素Ⅱ的相关性%Correlating endothelin releasing function of vascular endothelial cells to tea polyphenols and angiotensin Ⅱ

    Institute of Scientific and Technical Information of China (English)

    刘玉; 孙运峰; 马贵喜; 李靖; 蒙果; 韩磊; 刘昕; 李鸣皋

    2008-01-01

    张素Ⅱ组显著下降(P<0.01);而低浓度茶多酚在各作用时间点均较高浓度茶多酚组及血管紧张素Ⅱ组显著下降(P<0.01).结论:茶多酚对血管紧张素Ⅱ所致血管内皮细胞分泌内皮素的功能具有抑制作用,提示茶多酚对血管内皮细胞具有保护作用;且低浓度茶多酚的保护作用要强于高浓度茶多酚.%BACKGROUND: Angiotensin Ⅱ (Ang-Ⅱ) can stimulate vascular endothelial cells to excrete endothelin, a kind of potent vasoconstrictor. The content of endothelin in blood or cell culture media directly reflects the function and injured status of vascular endothelial cells. Therefore, it is significant for strengthening vascular endothelial cells to resist the injured factors. Tea polyphenols is a mainly active component of tea, and it is considered as a reagent for anti-atherosclerosis, protecting injuries of vascular endothelial cells and preventing cardiovascular diseases. OBJECTIVE: To observe the effects of tea polyphenols in different concentrations on endothelin content in vascular endothelial cells induced by Ang-Ⅱ at various time points through establishing Ang-Ⅱ-induced vascular endothelial cell injury models and further to investigate the protective effect of tea polyphenols on vascular endothelial cells. DESIGN: Observational study.SETTING: Aerospace and Diving Medical Center, Navy General Hospital of Chinese PLA.MATERIALS: The experiment was carried out in Laboratory of Aerospace and Diving Medical Center, Navy General Hospital of Chinese PLA from March to September 2005. Main materials were detailed as follows: Ang-Ⅱ (Sigma Company), tea polyphenols (Department of Tea Science, Zhejiang University) and vascular endothelial cells (human large artery vascular endothelial cell system, CBI Company, USA).METHODS: Cultured vascular endothelial cells were divided into 4 groups: ① Control group: The normal culture media was added in the isopyknic vascular endothelial cells, and 100 μL supernatant was extracted

  5. Rancimat法比较茶多酚、迷迭香及BHA+BHT对棕榈油氧化稳定性的影响%Study on the Effect of Anti-oxidation of Rosemary,Tea Polyphenols and BHA&BHT on Oxidative Stability of Palm Oil by Rancimat Method

    Institute of Scientific and Technical Information of China (English)

    刘祥; 欧锦强; 吴畏

    2012-01-01

    This paper studied on the effect of anti-oxidation of rosemary,tea polyphenols and composite stabilizer of BHABHT on oxidative stability of palm oil by the method of Rancimat.The induction time of samples which have different ratios anti-oxidation of rosemary,tea polyphenols and composite stabilizer of BHABHT was determined,the results showed that the anti-oxidation of antioxidants in palm oil was rosemary150 mg/kgtea polyphenols2 000 mg/kgrosemary 100 mg/kgBHT 60 mg/kg+BHA 60 mg/kgrosemary 50 mg/kgrosemary 30 mg/kgtea polyphenols500 mg/kg.Rosemary 50 mg/kg had better selectivity in the comparison,it has great prospect of application.%比较天然迷迭香、茶多酚与复配BHA和BHT对油脂氧化稳定性的影响,通过Rancimat法快速测定了添加不同配比的迷迭香、茶多酚及复配BHA和BHT的棕榈油样品的诱导时间,结果表明:在棕榈油中抗氧化稳定效果分别为迷迭香150 mg/kg〉茶多酚2 000mg/kg〉迷迭香100 mg/kg〉BHT 60 mg/kg+BHA 60 mg/kg〉迷迭香50 mg/kg〉迷迭香30 mg/kg〉茶多酚500 mg/kg。迷迭香50 mg/kg的抗氧化性能与复配BHT和BHA效果相近,具备较好的应用前景。

  6. Green synthesis of iron nanoparticles by various tea extracts: Comparative study of the reactivity

    Science.gov (United States)

    Huang, Lanlan; Weng, Xiulan; Chen, Zuliang; Megharaj, Mallavarapu; Naidu, Ravendra

    2014-09-01

    Iron nanoparticles (Fe NPs) are often synthesized using sodium borohydride with aggregation, which is a high cost process and environmentally toxic. To address these issues, Fe NPs were synthesized using green methods based on tea extracts, including green, oolong and black teas. The best method for degrading malachite green (MG) was Fe NPs synthesized by green tea extracts because it contains a high concentration of caffeine/polyphenols which act as both reducing and capping agents in the synthesis of Fe NPs. These characteristics were confirmed by a scanning electron microscope (SEM), UV-visible (UV-vis) and specific surface area (BET). To understand the formation of Fe NPs using various tea extracts, the synthesized Fe NPs were characterized by SEM, X-ray energy-dispersive spectrometer (EDS), and X-ray diffraction (XRD). What emerged were different sizes and concentrations of Fe NPs being synthesized by tea extracts, leading to various degradations of MG. Furthermore, kinetics for the degradation of MG using these Fe NPs fitted well to the pseudo first-order reaction kinetics model with more than 20 kJ/mol activation energy, suggesting a chemically diffusion-controlled reaction. The degradation mechanism using these Fe NPs included adsorption of MG to Fe NPs, oxidation of iron, and cleaving the bond that was connected to the benzene ring.

  7. Dietary polyphenols exert neuroprotective effects by attenuating neuronal and astrocytic damage in cerebral ischemia

    Science.gov (United States)

    Polyphenols are natural substances with variable phenolic structures and are found in vegetables, fruits, grains, bark, roots, tea, and wine. There are over 8000 polyphenolic structures identified in plants, but edible plants contain only several hundred polyphenolic structures. Recent interest in...

  8. Green tea polyphenol (−)-epigallocatechin-3-gallate triggered hepatotoxicity in mice: Responses of major antioxidant enzymes and the Nrf2 rescue pathway

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Dongxu; Wang, Yijun; Wan, Xiaochun [Key Laboratory of Tea Biochemistry & Biotechnology, School of Tea & Food Science, Anhui Agricultural University, Hefei, Anhui 230036 (China); Yang, Chung S. [Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Zhang, Jinsong, E-mail: zjs@ahau.edu.cn [Key Laboratory of Tea Biochemistry & Biotechnology, School of Tea & Food Science, Anhui Agricultural University, Hefei, Anhui 230036 (China)

    2015-02-15

    (−)-Epigallocatechin-3-gallate (EGCG), a constituent of green tea, has been suggested to have numerous health-promoting effects. On the other hand, high-dose EGCG is able to evoke hepatotoxicity. In the present study, we elucidated the responses of hepatic major antioxidant enzymes and nuclear factor erythroid 2-related factor 2 (Nrf2) rescue pathway to high-dose levels of EGCG in Kunming mice. At a non-lethal toxic dose (75 mg/kg, i.p.), repeated EGCG treatments markedly decreased the levels of superoxide dismutase, catalase, and glutathione peroxidase. As a rescue response, the nuclear distribution of Nrf2 was significantly increased; a battery of Nrf2-target genes, including heme oxygenase 1 (HO1), NAD(P)H:quinone oxidoreductase 1 (NQO1), glutathione S-transferase (GST), and those involved in glutathione and thioredoxin systems, were all up-regulated. At the maximum tolerated dose (45 mg/kg, i.p.), repeated EGCG treatments did not disturb the major antioxidant defense. Among the above-mentioned genes, only HO1, NQO1, and GST genes were significantly but modestly up-regulated, suggesting a comprehensive and extensive activation of Nrf2-target genes principally occurs at toxic levels of EGCG. At a lethal dose (200 mg/kg, i.p.), a single EGCG treatment dramatically decreased not only the major antioxidant defense but also the Nrf2-target genes, demonstrating that toxic levels of EGCG are able to cause a biphasic response of Nrf2. Overall, the mechanism of EGCG-triggered hepatotoxicity involves suppression of major antioxidant enzymes, and the Nrf2 rescue pathway plays a vital role for counteracting EGCG toxicity. - Highlights: • EGCG at maximum tolerated dose does not disturb hepatic major antioxidant defense. • EGCG at maximum tolerated dose modestly upregulates hepatic Nrf2 target genes. • EGCG at toxic dose suppresses hepatic major antioxidant enzymes. • EGCG at non-lethal toxic dose pronouncedly activates hepatic Nrf2 rescue response. • EGCG at

  9. Green tea polyphenol (−)-epigallocatechin-3-gallate triggered hepatotoxicity in mice: Responses of major antioxidant enzymes and the Nrf2 rescue pathway

    International Nuclear Information System (INIS)

    (−)-Epigallocatechin-3-gallate (EGCG), a constituent of green tea, has been suggested to have numerous health-promoting effects. On the other hand, high-dose EGCG is able to evoke hepatotoxicity. In the present study, we elucidated the responses of hepatic major antioxidant enzymes and nuclear factor erythroid 2-related factor 2 (Nrf2) rescue pathway to high-dose levels of EGCG in Kunming mice. At a non-lethal toxic dose (75 mg/kg, i.p.), repeated EGCG treatments markedly decreased the levels of superoxide dismutase, catalase, and glutathione peroxidase. As a rescue response, the nuclear distribution of Nrf2 was significantly increased; a battery of Nrf2-target genes, including heme oxygenase 1 (HO1), NAD(P)H:quinone oxidoreductase 1 (NQO1), glutathione S-transferase (GST), and those involved in glutathione and thioredoxin systems, were all up-regulated. At the maximum tolerated dose (45 mg/kg, i.p.), repeated EGCG treatments did not disturb the major antioxidant defense. Among the above-mentioned genes, only HO1, NQO1, and GST genes were significantly but modestly up-regulated, suggesting a comprehensive and extensive activation of Nrf2-target genes principally occurs at toxic levels of EGCG. At a lethal dose (200 mg/kg, i.p.), a single EGCG treatment dramatically decreased not only the major antioxidant defense but also the Nrf2-target genes, demonstrating that toxic levels of EGCG are able to cause a biphasic response of Nrf2. Overall, the mechanism of EGCG-triggered hepatotoxicity involves suppression of major antioxidant enzymes, and the Nrf2 rescue pathway plays a vital role for counteracting EGCG toxicity. - Highlights: • EGCG at maximum tolerated dose does not disturb hepatic major antioxidant defense. • EGCG at maximum tolerated dose modestly upregulates hepatic Nrf2 target genes. • EGCG at toxic dose suppresses hepatic major antioxidant enzymes. • EGCG at non-lethal toxic dose pronouncedly activates hepatic Nrf2 rescue response. • EGCG at

  10. Investigation and Comparison of the Aroma Components in Guangdong Black Tea%广东红茶香气成分的比较研究

    Institute of Scientific and Technical Information of China (English)

    王秋霜; 陈栋; 许勇泉; 尹军峰

    2012-01-01

    为了研究广东地方红茶香气物质并确定主要呈香特征,本研究采用顶空固相微萃取方法结合气质联用技术准确地鉴定了红茶样品中香气物质种类及其对总体香气的贡献.结果表明,研究鉴定了50多种香气化合物,主要包含醇类、酯类、醛类、酮类和烯类、烷烃类等,其中醇类是最主要的物质,其次是酯类和醛类.所体现的花香、甜香、果香是广东红茶的香气特征.香气化合物中芳樟醇、橙花醇、水杨酸甲酯、氧化芳樟醇、壬醛是五种最主要的呈香物质,平均含量分别占总香气的37.55%、11.90%、8.89%、5.4%和5.21%.英德系列红茶中醇类物质最多;白毛系列红茶中醛类、酮类物质最多;烯类物质以单丛和福建系列红茶含量较高.%For the purpose of studying the volatile aroma components in Guangdong black tea and determine the main aroma components, the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds in black tea samples and their contribution to the total aroma. Results showed that more than 50 aroma compounds were identified including alcohol, ester, aldehyde, ketone, terpene, hydrocarbon etc., among which the alcohol was the most important compounds and ester and aldehyde were next to flower-like, fruit-like, refreshing and sweet aroma were the main character of Guangdong black tea. Linalool, gerariol, methyl salicylate, linalool oxide and nonanal were the five most important compounds, representing 37.55%, 11.90%, 8.89%, 5.4% and 5.21% of the total aroma respectively. The alcohol was the most important compounds in Yingde tea population, the contents of aldehyde and ketone were more in Baimao tea population. Dancong and Fujian tea plant contained more terpene compounds.

  11. Hydrolysis of green tea residue protein using proteolytic enzyme derived from Aspergillus oryzae.

    Science.gov (United States)

    Xu, Yong-Quan; Zhong, Xiao-Yu; Chen, Su-Qin; Yin, Jun-Feng

    2013-02-01

    Free amino acids are important chemical components which impact the taste of green tea infusion. The hydrolysis of water-insoluble protein in the green tea residue helps to increase the contents of free amino acids components except theanine. Studies indicate that the hydrolysis of the tea protein could be restricted due to interaction of polyphenols with protein. The experiment indicates that the hydrolysis of tea protein by protease is the main trend when the polyphenols concentration is lower than 5 mg ml(-1), however, the proteins (including tea protein and protease) would interact with polyphenoles instead of hydrolysis when the concentration of polyphenols is higher than 5 mg ml(-1). The hydrolysis of tea protein is absolutely restrained when concentration comes to 10 mg ml(-1). PMID:24425904

  12. [Historical consideration of tea trees and tea flowers, especially regarding the use of tea flowers as food].

    Science.gov (United States)

    Harima, Shoichi; Yoshikawa, Masayuki; Tokuoka, Kiyoshi

    2008-01-01

    1400. The custom of tea serving continues today, and the tea flower continues to be used according to the demand. After that, it seems that "FURICHA" (tea with bubbles) continued to be used for 640 years, until 1590 (AZUCHI-MOMOYAMA period) because MANCHARAZU was regarded as important in religion. Then, it is believed that the customs of "BOTEBOTECHA" and/or"BATABATACHA" succeeded traditionally. The culture of both tea drinking and cooking, each development course, existed respectively from the relations of tea and food. The state that was accompanied with neither tea nor cooking, such as "KISHUU-CHAGAYU (tea gruel)" and BOTEBOTECHA, continued for a while, but it was combined together as "ICHIJUU-ICHISSA (one soup one tea)" of "KAISEKIRYOURI (tea-ceremony dish)." It is noteworthy that the tea flower is used as preserved food seasoned with "MISO (fermented soybean paste)" and "TSUKUDANI (boiled food in sweetened soy sauce)." Even though old documents about the use of tea flowers as food in China were not found, according to literature in the past ten years, seven descriptions were found that the tea flower was used for food provided alone or with black tea, oolong tea or green tea. As for tea leaves and tea flowers being used as medicine, drink or food in Japan, as well as in neighboring countries which belong to the Lucidophyllous forest zone. The process that they were regarded as important with the time was investigated and reported here. PMID:19227653

  13. 武夷名丛“向天梅”夏暑红茶适制性研究%Adaptability of Processing into Black Tea by Wuyi Mingcong“Xian Tianmei”

    Institute of Scientific and Technical Information of China (English)

    王飞权; 冯花; 罗盛财; 洪永聪; 吴玉琼; 钟兰馨; 张见明

    2013-01-01

      以武夷名丛“向天梅”夏暑茶鲜叶为原料,参照传统工夫红茶初制工艺,研究了“向天梅”夏暑茶鲜叶红茶的适制性,结合感官审评、生化成分和香气成分的测定和分析得知:武夷名丛“向天梅”夏暑茶鲜叶较适宜加工红茶。制得的夏暑红茶,水浸出物、氨基酸和茶红素含量分别为30.99%、1.31%和5.10%,香气含量占提取物总量的87.76%,其中共鉴定出9类160种化合物,香气甜浓,滋味甜醇,具备较好的红茶品质。%With summer fresh tea leaves of Wuyi Mingcong"Xian Tianmei", traditional processing technology of Gongfu black tea was refered, adaptability of processing into black tea by Wuyi Mingcong"Xian Tianmei"was studied. Combining sensory evaluation with chemical and aromatic components measurement and analysis. The conclusion was got: the summer fresh tea leaves of Wuyi Mingcong "Xian Tianmei" was suitable for processing of black tea. The summer black tea was then gained with better quality that the content of water extracts, amino acids and thearubigins were 30.99%, 1.31%and 5.10%, the content of aromatic components occupied 87.76%of the total content of extracts , about 9 classes of 160 different compounds were isolated and identified, the aroma was sweet and thick, the taste was sweet and pure.

  14. Quantitative Analysis of Phthalates Plasticizers in Traditional Egyptian Foods (Koushary and Foul Medams), Black Tea, Instant Coffee and Bottled Waters by Solid Phase Extraction-Capillary Gas Chromatography-Mass Spectroscopy

    OpenAIRE

    Abdallah S. Ammar; Mahmoud A. Mohamed

    2008-01-01

    In the present study, method of solid-phase extraction followed by capillary gas chromatography coupled to mass spectrometry (SPE-GC-MS) was used for quantitative analysis of trace levels of phthalates in the most tow Egyptians traditional food (foul medams and koushary) and drinks (black tea and instant black coffee) and bottled water samples.Method performance was evaluated in terms of accuracy, linearity, limits of detection and recovery. Also the practical application of extraction ...

  15. Polyphenols as active ingredients for cosmetic products.

    Science.gov (United States)

    Zillich, O V; Schweiggert-Weisz, U; Eisner, P; Kerscher, M

    2015-10-01

    Polyphenols are secondary plant metabolites with antioxidant, anti-inflammatory and anti-microbial activity. They are ubiquitously distributed in the plant kingdom; high amounts contain, for example, green tea and grape seeds. Polyphenolic extracts are attractive ingredients for cosmetics and pharmacy due to their beneficial biological properties. This review summarizes the effects of polyphenols in the context of anti-ageing activity. We have explored in vitro studies, which investigate antioxidant activity, inhibition of dermal proteases and photoprotective activity, mostly studied using dermal fibroblasts or epidermal keratinocytes cell lines. Possible negative effects of polyphenols were also discussed. Further, some physicochemical aspects, namely the possible interactions with emulsifiers and the influence of the cosmetic formulation on the skin delivery, were reported. Finally, few clinical studies, which cover the anti-ageing action of polyphenols on the skin after topical application, were reviewed. PMID:25712493

  16. Simultaneous Separation and Purification of Tea Bioactives from summer Green Tea by Column Chromatography

    International Nuclear Information System (INIS)

    In the present study, feasibility of producing high quality of tea polyphenols, the anine and caffeine from the extract of summer green tea by column chromatography was investigated. Crude extract of summer green tea obtained by hot water extraction was passed through columns of polyamide and NKA-II macroporous resin respectively, resulting in tea polyphenols-enriched fraction (96.27 +- 1.78%), the anine-enriched fraction (99.02 +- 0.43%) and caffeine-enriched fraction (99.25 +- 0.36%). The recovery rates for tea polyphenols, the anine and caffeine were 72.42 +- 1.41%, 66.12 +- 1.66% and 62.07 +- 2.17%, respectively. The separation procedure allowed the production of such products by decreasing impurities and avoiding the use of poisonous organic solvent. The results suggested that it was possible to produce high-quality products of rich-in tea polyphenols, the anine and caffeine by a simple way. From industrial point of view, this novel method has many merits such as high efficiency of separation, low consumption of energy and environment-friendly procedure. (author)

  17. Structural Properties of Green Tea Catechins

    OpenAIRE

    Botten, Dominic; Fugallo, Giorgia; Fraternali, Franca; Molteni, Carla

    2015-01-01

    Green tea catechins are polyphenols which are believed to provide health benefits; they are marketed as health supplements and are studied for their potential effects on a variety of medical conditions. However, their mechanisms of action and interaction with the environment at the molecular level are still not well-understood. Here, by means of atomistic simulations, we explore the structural properties of four green tea catechins, in the gas phase and water solution: specifically, (-)-epiga...

  18. 祁门红茶烟用香料的制备%Study on the Preparation of Qimen Black Tea Flavor Used in Cigarette

    Institute of Scientific and Technical Information of China (English)

    李德国; 冯黎; 奚安; 鲍辰卿; 邱家丹

    2014-01-01

    The black tea flavor used in cigarette was made from Qimen black tea leaf by a special technique,including using macroporous resins.The product possessed the characteristic sweet flower aroma of Qimen black tea. The volatile components of the flavor were determined by GC-MS,and it was found that the main compounds were trans-geraniol,linalool and its ox-ides,salicylic acid methyl ester,nerol,phenethyl alcohol,benzyl alcohol,nerolidol,trans-2-hexenal,etc.,which were also important ingredients in tobacco. The adsorption effects of several different macroporous resins were compared. The obtained products were added into cigarettes as tobacco flavorings and evaluated by panelists,the results indicated that they could enrich the tobacco flavor,increase the sweet feeling,reduce the irritancy of smoke and improve after taste obviously.%以祁门红茶为原料通过特殊工艺制得具有祁门红茶特征茶香、明显花香香韵的烟用香料,并以气相色谱-质谱联用分析方法对其挥发性成分进行鉴定,发现其中反式香叶醇、芳樟醇及其氧化物、水杨酸甲酯、橙花醇、苯乙醇、苯甲醇、橙花叔醇、反-2-己烯醛等是祁门红茶“祁门香”的特征香气成分。同时比较了几种常用的大孔树脂的吸附效果。所制备的祁门红茶烟用香料用于卷烟加香,能够明显丰富烟香,增加清甜香韵,减小刺激性和改善卷烟余味。

  19. 中国名优红茶香气成分的比较研究%Study on the Aroma Components in Chinese Famous Black Tea

    Institute of Scientific and Technical Information of China (English)

    王秋霜; 陈栋; 许勇泉; 尹军峰

    2013-01-01

    为了研究中国名优红茶挥发性香气物质并确定主要呈香特征,采用顶空固相微萃取方法(HS-SPME)提取红茶样品中的挥发性物质,用气相色谱/质谱联用技术(GC-MS)结合挥发性物质的保留指数(RI)鉴定红茶样品中香气物质种类及其对总体香气的贡献.结果表明,鉴定出约60种香气化合物,主要包含醇类、酯类、醛类、酮类和烯类、烷烃类等,其中醇类是国内名优红茶香气中最主要的物质,其次是酯类和醛类.香气化合物中芳樟醇、橙花醇、水杨酸甲酯、氧化芳樟醇是最主要的呈香物质,平均含量分别占总香气的39.48%、17.5%、8.21%、4.74%.所体现的花香、甜香、果香是中国红茶的香气特征.%With the aim to study the volatile aroma component of native black tea and determine the main aroma characters, the headspace solid-phase micro-extraction (HS-SPME) method was adopted to extract the volatiles and gas chromatography-mass spectrometry(GC-MS) coupled with retention index(RI) of the volatiles were used to determine the volatiles variety in black tea samples and their contribution to the total aroma. The result showed that more than fifty aroma compounds were determined including alcohol, ester, aldehyde, azulene ketones and terpenes, hydrocarbon and etc., among which the alcohol was the most important compounds and ester and aldehyde were next to it. Li nalool, ger-ariol, methl salcylate, linalool oxide were the most important compounds, representing 39.48%, 17.5%, 8.21% and 4.74% of the total aroma respectively. Flower-like, fruit-like, refreshing fragrance and sweet aroma were the main character of native black tea.

  20. Effect of green tea extract antioxidant polypHenols in cosmetics%绿茶提取物茶多酚在化妆品中的抗氧化效果研究

    Institute of Scientific and Technical Information of China (English)

    高彤彤; 晏志勇

    2015-01-01

    Objective To investigate the polypHenols(green tea extract)antioxidant effect in cosmetics. Methods Select 70 patients with skin aging,they were randomly divided into two groups, the observation group and the control group,35 cases in each group.After daily cleansing,the observation group were smear containing polypHenols(green tea extract)ointment and appropriate massage.Patients in the control group applied the same blank ointment base(excluding polypHenols), also make the appropriate massage,the same as the observation group.Its clear DPPH radical, hydroxyl radicals and superoxide anion was measured,skin antioxidant effects,symptom scores before and after facial aging and adverse events were compared after using 2 months.In vitro evaluation take VitC positive control method,measuring their ability to scavenge DPPH radicals, hydroxyl radicals and superoxide anion. Results Green tea extract has better scavenging DPPH radical,hydroxyl radical and superoxide anion using VitC as the positive control.After using the ointment,observation group total effective rate was significantly higher,the difference was statistically significant(P<0.05).For the improvement of the patient's symptoms score, the observation group and the control group after treatment scores were decreased compared with before treatment,the differences were statistically significant(P<0.05),scores of the observation group was significantly improved higher than patients in the control group,the difference was statistically significant(P<0.05). No significant adverse reactions. Conclusions PolypHenols(green tea extract)has a good antioxidant in cosmetics,which can combine with the body of free radicals into inert substances and prevent the radical reaction in vivo,to skin aging has a good therapeutic effect,and its natural extracts, safe and can be widely used in the cosmetic preparation.%目的:探讨绿茶提取物茶多酚在化妆品中的抗氧化效果.方法:选取皮肤衰老患者70例,随机

  1. Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs.

    Science.gov (United States)

    Kiselova, Yoana; Ivanova, Diana; Chervenkov, Trifon; Gerova, Daniela; Galunska, Bistra; Yankova, Tatyana

    2006-11-01

    The water phase antioxidant activity of extracts from 23 Bulgarian medicinal plants was studied in relation to their polyphenol content in comparison with mate, black tea, honeybush and rooibos foreign species. Antioxidant activity was measured by the ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) cation radical decolorization assay, and the total polyphenol content was assayed according to the Folin-Ciocalteu method. Five Bulgarian plant extracts exhibited higher antioxidant activity than that of mate, which is 21.7% of all Bulgarian herbs included in this study. These were Alchemilla vulgaris L. (4.79 +/- 0.14 mm), Sambucus ebulus L. (4.03 +/- 0.07 mm), Mentha spicata L. (3.90 +/- 0.03 mm), Fragaria vesca L. (3.74 +/- 0.06 mm), Crataegus monogyna Jacq. (3.63 +/- 0.05 mm). Another eight Bulgarian medicinal plant extracts exhibited an intermediate antioxidant activity - lower than that of mate and higher than that of honeybush, which makes 34.8% of all Bulgarian herbs included in the study. More than half of the herbal extracts included in the present study exhibited antioxidant activity higher than or comparable to the reference foreign plants. A positive correlation (r = 0.92) between antioxidant activity and polyphenol content was found, suggesting that the antioxidant capacity of the aqueous plant extracts is due to a great extent to their polyphenols. PMID:16906640

  2. 福建大宗红茶市场现状分析与对策——以立顿成功经验为鉴%Countermeasure and Market Status Analysis on Staple Black Tea in Fujian -- Take the Successful Experiences of Lipton as Reference

    Institute of Scientific and Technical Information of China (English)

    王红平; 刘艺蕾; 曾治浩

    2012-01-01

    Staple tea product caters to modern urbanite's consumption habits, and it will dominate the tea market in the future. This paper summarized the current situation and the existing problems of black tea market in Fujian, and analyzed the successful experiences on market development of Lipton black tea. Finally, the countermeasures to promote the development of staple black tea market in Fujina were put forward.%大宗茶产品符合现代都市人的消费习惯,在未来茶叶市场将占主导地位。该文总结了福建大宗红茶市场的现状及面临的问题,分析了立顿大宗红茶市场开发的成功经验,并据此提出促进福建大宗红茶市场发展的对策思路。

  3. 茶多酚对饮茶型氟中毒大鼠关节软骨氧化损伤的保护作用%Protective role of tea polyphenols in oxidative stress damage of the rat articular cartilage tissue caused by brick-tea fluorosis

    Institute of Scientific and Technical Information of China (English)

    张微; 高彦辉; 林林; 孙殿军

    2009-01-01

    Objective To explore the protective mechanism of tea polyphenols (TPs) ion oxidative stress damage of the rat articular cartilage tissue caused by brick-tea fluorosis. Methods One hundred and twenty wistar male rats were randomly divided into 6 groups according to body mass: fluoride group with drinking water containing 100.00 mg/L F-, fluoride plus TPs group treated with 100.00 mg/L F- and 10.0 g/L TPs, fluoride plus aluminum group fed with 100.00 mg/L F- and 200.00 mg/L Al3+, fluoride plus aluminium and TPs group treated with 100.00 mg/L F-,200.O0 mg/L Al3+ and 10.0 g/L TPs;brick-tea group treated with drinking water containing 100.00 mg/L F-,215.00 mg/L Al3+ and 9.2 g/L TPs, which was steeped by the brick-tea;control group treated with tap water. The animals were bred for three months and then sacrificed. The level of SOD,T-AOC and MDA in blood serum were detected,also the level of NO and cytokine IL-1β and IL-6, the expression of iNOS mRNA and protein in articular cartilage were respectively analyzed by RT-PCR and immunohistochemistry. Results Blood serum SOD level in the fluoride plus aluminum and TPs group[(664.009 ± 29.589)kU/L] was higher compared with that in the fluoride group[(625.328 ± 27.199)kU/L], fluoride plus aluminum group[(652.282±13.926)kU/L], although no statistically significant differences was found(P > 0.05) ;blood serum T-AOC level of the fluoride plus TPs, fluoride plus aluminum and TPs group, brick tea group[(10.874 ± 0.721), (11.871 ± 0.941), (10.380 ± 2.747)kU/L] was higher compared with fluoride group, fluoride plus aluminum group [(8.849 ± 1.887), (8.210 ± 1.740)kU/L], the differences all being statistically significant(P 0.05);the blood serum IL-6 level of fluoride plus aluminum and TPs group, brick-tea group[(7.231 ± 0.596), (7.325 ± 0.290)ng/L] had statistical differences compared with fluoride plus aluminum[(8.256 ± 0.635)ng/L, P 0.05);氟+茶多酚组、氟+铝+茶多酚组、砖茶组T-AOC水平[(10.874±0.721)、(11

  4. Metabolism of green tea catechins in the human small intestine

    OpenAIRE

    Schantz, Markus; Erk, Thomas; Richling, Elke

    2010-01-01

    Abstract Numerous studies have shown that green tea polyphenols can be degraded in the colon, and there is abundant knowledge about the metabolites of these substances that appear in urine and plasma after green tea ingestion. However, there is very little information on the extent and nature of intestinal degradation of green tea catechins in humans. Therefore, the aim of this study presented here was to examine in detail the microbial metabolism and chemical stability of these po...

  5. Study on the optical limiting properties of the mixed liquid of carbon black suspensions and green tea solution by multi-pulse laser

    Institute of Scientific and Technical Information of China (English)

    Niu Yan-Xiong; Yang Hai-Lin; Zhang Peng; Shen Xue-Ju; Jiang Nan; Chen Yan; Tang Fang

    2008-01-01

    The optical limiting properties of the mixed liquid of carbon black suspensions(CBS)and green tea solution were studied by using an 8 ns laser pulse at 532nm.The optical limiting effects of the CBS and mixed liquid have been compared between 5 and 10 Hz repetition frequencies with nanosecond laser pulse.The experimental results indicate that the optical limiting threshold of the sample with the incidence laser at 10 Hz repetition frequency is lower than at 5 Hz repetition frequency.The possible reasons for the influence of the repetition frequency on the samples are discussed.And by observing the optical radiant distributions when the laser pulse passing through different samples,a possible mechanism for the observed effects is suggested.At the same time,the result shows that the optical limiting of CBS is the dominant factor to optical limiting ofthe mixed liquid.

  6. CLASSICAL AREAS OF PHENOMENOLOGY: Study on the optical limiting properties of the mixed liquid of carbon black suspensions and green tea solution by multi-pulse laser

    Science.gov (United States)

    Niu Yan-, Xiong; Yang, Hai-Lin; Zhang, Peng; Shen, Xue-Ju; Jiang, Nan; Chen, Yan; Tang, Fang

    2008-09-01

    The optical limiting properties of the mixed liquid of carbon black suspensions (CBS) and green tea solution were studied by using an 8 ns laser pulse at 532 nm. The optical limiting effects of the CBS and mixed liquid have been compared between 5 and 10 Hz repetition frequencies with nanosecond laser pulse. The experimental results indicate that the optical limiting threshold of the sample with the incidence laser at 10 Hz repetition frequency is lower than at 5 Hz repetition frequency. The possible reasons for the influence of the repetition frequency on the samples are discussed. And by observing the optical radiant distributions when the laser pulse passing through different samples, a possible mechanism for the observed effects is suggested. At the same time, the result shows that the optical limiting of CBS is the dominant factor to optical limiting of the mixed liquid.

  7. Study on the optical limiting properties of the mixed liquid of carbon black suspensions and green tea solution by multi-pulse laser

    International Nuclear Information System (INIS)

    The optical limiting properties of the mixed liquid of carbon black suspensions (CBS) and green tea solution were studied by using an 8 ns laser pulse at 532 nm. The optical limiting effects of the CBS and mixed liquid have been compared between 5 and 10 Hz repetition frequencies with nanosecond laser pulse. The experimental results indicate that the optical limiting threshold of the sample with the incidence laser at 10 Hz repetition frequency is lower than at 5 Hz repetition frequency. The possible reasons for the influence of the repetition frequency on the samples are discussed. And by observing the optical radiant distributions when the laser pulse passing through different samples, a possible mechanism for the observed effects is suggested. At the same time, the result shows that the optical limiting of CBS is the dominant factor to optical limiting of the mixed liquid. (classical areas of phenomenology)

  8. Spectroscopic fingerprint of tea varieties by surface enhanced Raman spectroscopy.

    Science.gov (United States)

    Buyukgoz, Guluzar Gorkem; Soforoglu, Mehmet; Basaran Akgul, Nese; Boyaci, Ismail Hakki

    2016-03-01

    The fingerprinting method is generally performed to determine specific molecules or the behavior of specific molecular bonds in the desired sample content. A novel, robust and simple method based on surface enhanced Raman spectroscopy (SERS) was developed to obtain the full spectrum of tea varieties for detection of the purity of the samples based on the type of processing and cultivation. For this purpose, the fingerprint of seven different varieties of tea samples (herbal tea (rose hip, chamomile, linden, green and sage tea), black tea and earl grey tea) combined with silver colloids was obtained by SERS in the range of 200-2000 cm(-1) with an analysis time of 20 s. Each of the thirty-nine tea samples tested showed its own specific SERS spectra. Principal Component Analysis (PCA) was also applied to separate of each tea variety and different models developed for tea samples including three different models for the herbal teas and two different models for black and earl grey tea samples. Herbal tea samples were separated using mean centering, smoothing and median centering pre-processing steps while baselining and derivatisation pre-processing steps were applied to SERS data of black and earl grey tea. The novel spectroscopic fingerprinting technique combined with PCA is an accurate, rapid and simple methodology for the assessment of tea types based on the type of processing and cultivation differences. This method is proposed as an alternative tool in order to determine the characteristics of tea varieties. PMID:27570296

  9. Extraction Efficiency of Elements in Chinese Tea Infusions

    OpenAIRE

    Minami, Takeshi; KURUMANO, Kosuke; KAMEYAMA, Shuhei; YOSHIDA, Mai

    2012-01-01

    [Abstract] The concentrations of elements in Chinese tea infusions after both one-minute and overnight extractions were measured and the contents were compared with those of tea leaves. Leaves from 13 Chinese tea plants in ten provinces in China were collected; seven were green tea, four were oolong tea, and two were black tea. When seven major elements, Ca, Fe, K, Mg, P, S, and Zn, were measured, the contents in tea leaves did not differ, except for Zn. Ca content in one-minute infusion of g...

  10. Time-dependent extraction kinetics of infused components of different Indian black tea types using UV spectroscopy

    Directory of Open Access Journals (Sweden)

    Asir Gani

    2016-12-01

    Full Text Available Time-dependent aqueous extraction of six tea types was carried out with leaf–water–ratio of 0.5 g/100 ml, temperature of extraction 90°C and time of extraction ranging from 1 to 10 min. UV–vis spectroscopic analysis in the range varying from 220 to 900 nm of the aqueous tea extracts showed a prominent peak at 273 nm in the ultraviolet region which can be associated with n → π* electronic transition of caffeine molecules. Parabolic diffusion, Power law, hyperbolic, Weibull’s and Elovich’s models were fitted to represent the aqueous soluble component extraction behaviour for time-dependent extraction of aqueous extractables. Parabolic diffusion model, Power law and Elovich’s model were a close fit to the experimental data for all the selected tea types with correlation coefficients (R2 ranging 0.8029–0.9953, whereas hyperbolic and Weibull’s models showed poor fitness to represent the extraction behaviour of fanning and AO leaf, LD, fanning and dust, respectively, with R2 < 0.8, for time-dependent aqueous soluble component extraction.

  11. Identification of Green, Oolong and Black Teas in China via Wavelet Packet Entropy and Fuzzy Support Vector Machine

    Directory of Open Access Journals (Sweden)

    Shuihua Wang

    2015-09-01

    Full Text Available To develop an automatic tea-category identification system with a high recall rate, we proposed a computer-vision and machine-learning based system, which did not require expensive signal acquiring devices and time-consuming procedures. We captured 300 tea images using a 3-CCD digital camera, and then extracted 64 color histogram features and 16 wavelet packet entropy (WPE features to obtain color information and texture information, respectively. Principal component analysis was used to reduce features, which were fed into a fuzzy support vector machine (FSVM. Winner-take-all (WTA was introduced to help the classifier deal with this 3-class problem. The 10 × 10-fold stratified cross-validation results show that the proposed FSVM + WTA method yields an overall recall rate of 97.77%, higher than 5 existing methods. In addition, the number of reduced features is only five, less than or equal to existing methods. The proposed method is effective for tea identification.

  12. Antioxidant activity and nutrient release from polyphenol-enriched cheese in a simulated gastrointestinal environment.

    Science.gov (United States)

    Lamothe, Sophie; Langlois, Ariane; Bazinet, Laurent; Couillard, Charles; Britten, Michel

    2016-03-01

    Green tea polyphenols are recognized for their antioxidant properties and their effects on lipid digestion kinetics. Polyphenols are sensitive to degradation in the intestinal environment. Interactions with dairy proteins could modulate the stability and biological activity of polyphenols during digestion. The objective of this study was to evaluate the release of nutrients (polyphenols, fatty acids and peptides) and the antioxidant activity in polyphenol-enriched cheese containing different levels of calcium in a simulated gastrointestinal environment. The relationship between cheese matrix texture, matrix degradation and nutrient release during digestion was also studied. Green tea extract was added to milk at 0% or 0.1%, and cheeses were produced on a laboratory scale. The level of available calcium was adjusted to low (Ca(low)), regular (Ca(reg)) or high (Ca(high)) during the salting step of the cheese-making process. Cheeses were subjected to simulated digestion. The rate and extent of fatty acid release were 21% lower for Ca(low) cheese than for Ca(reg) and Ca(high) cheeses. The greater adhesiveness of Ca(low) cheese, which resulted in lower rates of matrix degradation and proteolysis, contributed to the reduced rate of lipolysis. The presence of green tea extract in cheese reduced the release of free fatty acids at the end of digestion by 7%. The addition of green tea extract increased cheese hardness but did not influence matrix degradation or proteolysis profiles. The formation of complexes between tea polyphenols and proteins within the cheese matrix resulted in a more than twofold increase in polyphenol recovery in the intestinal phase compared with the control (tea polyphenol extract incubated with polyphenol-free cheese). Antioxidant activity was 14% higher in the digest from polyphenol-enriched cheese than in the control. These results suggest that cheese is an effective matrix for the controlled release of nutrients and for the protection of green

  13. Polyphenols and antioxidant activities of Kombucha beverage enriched with Coffeeberry® extract

    Directory of Open Access Journals (Sweden)

    Essawet Najmi Ahmed

    2015-01-01

    Full Text Available Kombucha is a traditional beverage obtained by fermenting sweetened black tea with tea fungus, which represents a consortium of acetic acid bacteria and yeasts. Also, CoffeeBerry® products, which derived from the whole fruit of the coffee plant, are valuable ingredients with nutritional and health-enhancing potential. Samples of fermentation broths enriched with CoffeeBerry® extract and traditional Kombucha were analysed. The fermentation was performed in a bioreactor at 28±1°C for nine days. The results showed that the CoffeeBerry® extract has contributed to a faster fermentation of cultivation medium. Some individual polyphenolic compounds and catehins in fermentation broth samples were identified and quantified by High Performance Liquid Chromatography (HPLC. Among the bioactive compounds present in investigated samples obtained during Kombucha fermentation of the sweetened black tea enriched with CoffeeBerry® extract, chlorogenic acid (188.94-458.56 μg/mL was the predominant. The antioxidant activity of investigated samples was tested by measuring their ability to scavenge DPPH and reactive hydroxyl radicals by electron spin resonance (ESR spectroscopy. The scavenging activities on DPPH and hydroxyl radicals were increased with duration of fermentation. IC50 values for Kombucha fermentation broth enriched with CoffeBerry®, based on DPPH and hydroxyl radical scavenging activities, were in the range 26.33-170.13 μL/mL and 11.33-102.22 μL/mL, respectively.

  14. Extraction of anthocyanins and polyphenols from black rice (Oryza sativa L.) by modeling and assessing their reversibility and stability.

    Science.gov (United States)

    Pedro, Alessandra Cristina; Granato, Daniel; Rosso, Neiva Deliberali

    2016-01-15

    This study was aimed the extraction of total flavonoids, anthocyanins and phenolics, as well as the antioxidant activity of black rice (Oryza sativa) and to study the stability in relation to pH, light and copigmentation. Variations in temperature (10-50°C), time (20-80min), and solid-solvent ratio (1:15-1:45) were studied using a Box-Behnken design. The regression models were significant (Pphytic and gallic acids in the optimized extract exposed to light displayed an intermolecular copigmentation. The main anthocyanin identified in black rice was cyanidin-3-glucoside. PMID:26258696

  15. Antimicrobial activity of different tea varieties available in Pakistan.

    Science.gov (United States)

    Zakir, Muhammad; Sultan, Khush Bakht; Khan, Haroon; Ihsaanullah; Khan, Murad Ali; Fazal, Hina; Rauf, Abdur

    2015-11-01

    In this antimicrobial study, various extracts of Green and Black tea (Camellia sinensis) and Lemon grass (Cymbopogon citrates) were evaluated for antimicrobial activities against six bacterial strains including both human pathogenic bacteria (Escherichia coli, Pseudomonas aeuroginosa, Staphylococcus aureus and Salmonella typhi) and plant pathogenic bacteria (Erwinia carotovora, Agro bacterium tumifaciens) and one fungal strain Candida albicans by disc diffusion susceptibility method. Of human pathogens, P. aeruginosa was most susceptible to all three different tea varieties; though rest of the strains also demonstrated prominent sensitivity. In comparison, black tea extracts were less activities than green tea and lemon grass. However, all the three tea varieties illustrated profound activity against plant pathogenic bacteria. Similarly, when extracts of tea were tested against C. albicans, green tea and lemon grass exhibited significant activity while black tea was mostly inactive. PMID:26639502

  16. Effect of polyphenols on oxidative stress and mitochondrial dysfunction in neuronal death, brain edema, and cell swelling in cerebral ischemia

    Science.gov (United States)

    Polyphenols are natural substances with variable phenolic structures and are elevated in vegetables, fruits, grains, bark, roots, tea, and wine. while there are over 8000 polyphenolic structures identified in plants, edible plants contain only several hundred polyphenolic structures. In addition t...

  17. Role of green tea as an antioxidant in periodontal disease: The Asian paradox

    OpenAIRE

    Nugala, Babitha; Namasi, Ambalavanan; Emmadi, Pamela; Krishna, P. Mohana

    2012-01-01

    Green tea is a popular beverage nowadays and intake of green tea polyphenols has shown preventive effect against cancer and cardiovascular disease in experimental and epidemiologic studies. Several studies have suggested that green tea catechins, such as epigallocatechin gallate, inhibit periodontal pathogens and reduce the destruction of periodontal tissue. This paper aims to review the role of green tea in inflammatory diseases, especially in periodontal diseases.

  18. Effects of background and optical path length on predicting the contents of tea polyphenol with NIR models%背景和光程对茶多酚含量近红外预测模型的影响

    Institute of Scientific and Technical Information of China (English)

    王胜鹏; 龚自明; 何远军; 高士伟; 郑鹏程; 滕靖; 王雪萍; 叶飞; 卢素芳

    2015-01-01

    为比较空气和纯水背景的模型效果,扫描5个(2、3、5、8和10 mm)不同光程的红茶汤近红外光谱,应用排列组合原理和偏最小二乘法(PLS)建立2种背景下单光程和多光程组合茶多酚近红外预测模型,并用t-test检验模型预测效果。结果表明:单光程模型中,纯水背景与空气背景模型预测结果间无显著性差异;多光程组合模型中,空气背景模型要全部优于纯水背景模型,并且最佳组合模型((5+8)mm)具有比单光程模型更好的预测精度。空气背景茶多酚模型要优于纯水背景茶多酚模型。%In order to establish the stable models,the air and pure water background NIRS models were compared.2 mm,3 mm,5 mm,8 mm and 10 mm optical path length NIR spectra of black tea’s so-lution on air and pure water background were attained to set up single-optical and multi-optical path length NIR models through using partial least squares (PLS)and principle of permutation and combina-tion.The results of using NIR models to predict unknown samples of black tea were examined by t-test statistical method.The results showed that there was no difference between the NIR models with the same optical path length and different background.There was significant difference (P <0.05)between models with different optical path lengths on the pure water background.In the multi-optical path length models,the performances of NIR models on air background were better than that of the models on water background.The best NIR model combined 5 mm and 8 mm optical path length,whose predictable accu-racy was better than that of single-optical path length models.The NIRS models of air background was better than that of pure water background.

  19. 广东仁化白毛红茶香气组成的比较研究%Research and comparison of the aroma components in Guangdong renhua Baimao black tea

    Institute of Scientific and Technical Information of China (English)

    王秋霜; 陈栋; 许勇泉; 尹军峰

    2012-01-01

    For the purpose of studying the volatile aroma component in black tea processed with Guangdong native tea variety-Renhua Baimao tea as material and determining the main aroma components,the headspace solid-phase micro-extraction method and gas chromatography-mass spectrometry were used to determine the aroma compounds of 3 black tea products and their contribution to the total aroma. The results showed that more than 50 aroma compounds were identified including alcohol, ester,aldehyde, ketones,terpene, hydrocarbon and etc,among which the alcohol was the most important compound,ester and aldehyde were next to it. Predominant components in Renhua Baimao tea variety- black tea were linalool, methl salcylate, nonanal, Neroli and linalool oxide,representing 27.78% ,9.85% ,7.99% ,7.42% and 6.84% of the total aroma respectively. Baimao black tea contained more flower fruit-like(eg, linalool,nonanal, Neroli and etc). than Sri Lanka Wuwa black tea(CK3) and less than Yinghong 9(CK1). However,the fragrant oil compounds in Baimao black tea were less than CK3 and higher than CK1. It might be the reason that in sensory Baimao black tea had fresh and thick sweet aroma liked CK1 as well as persistent fine sharp aroma liked CK3.%为了探明以广东地方特色茶树品种-“仁化白毛茶”为原料加工而成的红茶之主要香气物质并确定其呈香特征.本研究采用顶空固相微萃取方法结合气质联用技术研究鉴定了3个红茶产品的香气物质种类及其对总体香气的贡献。结果表明,研究鉴定出50多种香气化合物,主要包含醇类、酯类、醛类、酮类、烯类和烷烃类等,其中醇类是最主要的物质,其次是酯类和醛类。仁化白毛茶树品种红茶的“主导香气成分”是芳樟醇、水杨酸甲酯、壬醛、橙花醇、氧化芳樟醇.平均含量分别占总香气的27.78%、9.85%、7.99%、7.42%和6.84%。仁化白毛红茶的

  20. Determination of lead, cadmium and arsenic in infusion tea cultivated in north of Iran

    OpenAIRE

    Shekoohiyan Sakine; Ghoochani Mahboobeh; Mohagheghian Azita; Mahvi Amir Hossein; Yunesian Masoud; Nazmara Shahrokh

    2012-01-01

    Abstract Tea is one of the most common drinks in all over the world. Rapid urbanization and industrialization in recent decades has increased heavy metals in tea and other foods. In this research, heavy metal contents such as lead (Pb), cadmium (Cd) and arsenic (As) were determined in 105 black tea samples cultivated in Guilan and Mazandaran Provinces in north of Iran and their tea infusions. The amount of heavy metals in black tea infusions were analyzed using Inductively Coupled Plasma Atom...

  1. Brewing and volatiles analysis of three tea beers indicate a potential interaction between tea components and lager yeast.

    Science.gov (United States)

    Rong, Lei; Peng, Li-Juan; Ho, Chi-Tang; Yan, Shou-He; Meurens, Marc; Zhang, Zheng-Zhu; Li, Da-Xiang; Wan, Xiao-Chun; Bao, Guan-Hu; Gao, Xue-Ling; Ling, Tie-Jun

    2016-04-15

    Green tea, oolong tea and black tea were separately introduced to brew three kinds of tea beers. A model was designed to investigate the tea beer flavour character. Comparison of the volatiles between the sample of tea beer plus water mixture (TBW) and the sample of combination of tea infusion and normal beer (CTB) was accomplished by triangular sensory test and HS-SPME GC-MS analysis. The PCA of GC-MS data not only showed a significant difference between volatile features of each TBW and CTB group, but also suggested some key compounds to distinguish TBW from CTB. The results of GC-MS showed that the relative concentrations of many typical tea volatiles were significantly changed after the brewing process. More interestingly, the behaviour of yeast fermentation was influenced by tea components. A potential interaction between tea components and lager yeast could be suggested. PMID:26616936

  2. 茶多酚醇质体的制备和体外透皮吸收研究%Preparation of tea polyphenols ethosomes and their in vitro percutaneous absorption study

    Institute of Scientific and Technical Information of China (English)

    陈元岭; 王丹; 王技; 李永吉; 王向涛

    2013-01-01

    目的:制备茶多酚醇质体,并对其进行皮肤渗透性评价.方法:采用注入法制备醇质体,马尔文Zeta电位仪测定其粒径及电位,紫外分光光度法测定包封率;采用改良Franz扩散池装置,以ICR小鼠背部皮肤作为渗透屏障,进行体外经皮渗透试验.结果:成功制备了茶多酚醇质体,平均粒径(162.2±7.1) nm,Zeta电位-(34.3±3.9) mV,包封率(37.43±1.90)%.体外试验中,茶多酚醇质体的稳态渗透速率是97.27 μg·cm-2·h-1,分别是茶多酚30%乙醇溶液、脂质体、水溶液的2.86,7.47,11.98倍,24 h累积渗透量排序为:茶多酚醇质体>30%乙醇溶液>脂质体>水溶液.结论:醇质体的制备方法可行,醇质体能够促进茶多酚的皮肤渗透.%Objective:To prepare tea polyphenols (TP) ethosomes,and investigate their percutaneous permeation in vitro.Methods:TP ethosomes were prepared by ethanol infusion method,and the encapsulation efficiency was determined by UV-spectrometry.The transdermal penetration of different TP dosage forms was evaluated in vitro using modified Franz diffusion cells method,with the back skin of ICR male rats as percutaneous permeation barrier.Results:TP ethosomes were successfully prepared with encapsulation efficiency of (37.43 ± 1.90) %,average particle size of (162.2 ± 7.1) nm,and Zeta potential of-(34.3 ± 3.9) mV.The steady state flux In vitro of TP from ethosome was 97.27 μg·cm-2· h-1,which was 2.86,7.47 and 11.98 times as that from 30% enthanol TP solution,TP liposomes and aqueous TP solution,respectively.The accumulated permeation amount in 24 h of different TP dosage forms was in the following order:ethosomes > 30% ethanol solution > liposomes > aqueous solution.Conclusions:The preparation technique of ethosomes is feasible,and it can enhance the penetration of TP through skin effectively.

  3. Green tea as a source of food antioxidants

    DEFF Research Database (Denmark)

    Yin, Jie

    Safe” (GRAS-status). In this PhD project, green tea rich in polyphenol compounds catechins was selected as the natural source of antioxidants. The aim of the project was to investigate green tea as a source of natural antioxidants for use in processed foods. First of all, the antioxidant effects of...... green tea catechins and green tea extract were investigated in terms of scavenging efficiency of the food-derived free radicals. The relevant experimental methods were based on electrochemical detection of oxygen consumption, electron spin resonance (ESR) spectroscopy, Rancimat test and liposome...... oxidation test. In addition, antioxidant interactions for individual green tea catechins or green tea extract in combination with endogenous antioxidant, α-tocopherol, were studied. The results showed that green tea catechins possess excellent antioxidant activity against the oxidative damage of lipids...

  4. Inhibition of wheat starch retrogradation by tea derivatives.

    Science.gov (United States)

    Zhang, Haihua; Sun, Binghua; Zhang, Shikang; Zhu, Yuejin; Tian, Yaoqi

    2015-12-10

    The effect of four industrial tea derivatives (tea polyphenols [TPS], tea water-soluble extracts [TSE], tea polysaccharides [TSS], and green tea powder [GTP]), on the retrogradation of wheat starch was investigated using texture profile analysis (TPA), differential scanning calorimetry (DSC), rapid viscosity analysis (RVA), and the α-amylase-iodine method. The addition of the four tea derivatives resulted in decreased hardness and increased cohesiveness of the starch gel as shown by the TPA test. The DSC data demonstrated an increase in the enthalpy change of starch gelatinization and a decrease in the enthalpy change of starch recrystallite dissociation. The RVA results indicated that the peak viscosity, representing the intermolecular forces of wheat starch, was reduced after addition of TPS, TSE, and TSS, respectively, but was increased by GTP. Furthermore, the half crystallization time in the Avrami equation almost doubled after the separate addition of the tea derivatives. PMID:26428142

  5. Backyard Teas.

    Science.gov (United States)

    Young, Darrell D.

    1996-01-01

    Describes plants commonly found in residential areas that can be used for making tea: chicory, chickweed, red clover, goldenrod, gill-over-the-ground, pineapple weed, plantain, self-heal, sheep sorrel, and wild strawberry. Includes proper plant name, areas where the plant grows, identifying plant features, what part is used in making tea, and tea…

  6. Tea Philosophy

    Institute of Scientific and Technical Information of China (English)

    Bai Shi

    2013-01-01

    A healthy lifestyle and intangible cultural heritage Every spring, Chinese indulge in drinking copious amounts of fresh green tea picked and processed in the country's southern mountain areas. In particular, several days before and after the Tomb Sweeping Day (April 4), tea trees blessed with

  7. Estimated Dietary Polyphenol Intake and Major Food and Beverage Sources among Elderly Japanese.

    Science.gov (United States)

    Taguchi, Chie; Fukushima, Yoichi; Kishimoto, Yoshimi; Suzuki-Sugihara, Norie; Saita, Emi; Takahashi, Yoshinari; Kondo, Kazuo

    2015-12-01

    Estimating polyphenol intake contributes to the understanding of polyphenols' health benefits. However, information about human polyphenol intake is scarce, especially in the elderly. This study aimed to estimate the dietary intake and major sources of polyphenols and to determine whether there is any relationship between polyphenol intake and micronutrient intake in healthy elderly Japanese. First, 610 subjects (569 men, 41 women; aged 67.3 ± 6.1 years) completed food frequency questionnaires. We then calculated their total polyphenol intake using our polyphenol content database. Their average total polyphenol intake was 1492 ± 665 mg/day, the greatest part of which was provided by beverages (79.1%). The daily polyphenol intake differed largely among individuals (183-4854 mg/day), also attributable mostly to beverage consumption. Coffee (43.2%) and green tea (26.6%) were the major sources of total polyphenol; the top 20 food items accounted for >90%. The polyphenol intake did not strongly correlate with the intake of any micronutrient, suggesting that polyphenols may exert health benefits independently of nutritional intake. The polyphenol intake in this elderly population was slightly higher than previous data in Japanese adults, and beverages such as coffee and green tea contributed highly to the intake. PMID:26690212

  8. Protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T ceils from vitiligo patients%茶多酚对CD8+T细胞杀伤白癜风患者黑素细胞的保护作用研究

    Institute of Scientific and Technical Information of China (English)

    欧阳杰; 吴纪龙; 周妙妮; 付丽芳; 许爱娥

    2013-01-01

    Objective To evaluate the protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T cells from vitiligo patients.Methods Skin tissue was resected from the margin of vitiligo lesions followed by the isolation and culture of CD8+ T lymphocytes,and from the normal skin of vitiligo patients followed by the isolation and culture of melanocytes.Flow cytometry was carried out to evaluate the purity of CD8+ T cells.The melanocytes were cocultured with the CD8 + T cells at different ratios followed by the evaluation of killing effect of CD8+ T cells.Various concentrations (200 and 400 μg/ml) of tea polyphenols were added into the co-culture system of CD8+ T cells and melanocytes at a ratio of 5 ∶ 1 followed by an additional culture of 48 hours.Then,flow cytometry was performed to detect the apoptosis in melanocytes in the coculture system.Results CD8+ T lymphocytes were successfully obtained from the marginal area of vitiligo lesions with a purity of more than 90%,which highly expressed the antigens CD137 and CD69.The coculture with CD8+ T cells markedly accelerated the apoptosis in melanocytes,while the accelerative effect was inhibited by tea polyphenols of 200 and 400 μg/ml.Conclusions The CD8+ T cells infiltrating the edge of vitiligo lesions display a potential destructive effect on autologous melanocytes from vitiligo patients,and tea polyphenols have a protective effect against the destruction of melanocytes by CD8+ T cells.%目的 探讨茶多酚对CD8+T细胞杀伤白癜风患者黑素细胞的保护作用.方法 取白癜风患者白斑边缘处皮片组织,培养CD8+T淋巴细胞,细胞纯度用流式细胞仪进行鉴定,通过和黑素细胞共培养验证其针对黑素细胞的杀伤作用.将不同浓度茶多酚加入CD8+T细胞和黑素细胞共培养的体系,用流式细胞仪检测黑素细胞的凋亡.结果 取白癜风皮损边缘表皮组织中成功培养CD8+T淋巴细胞,纯度达90%

  9. An Old Town on the Ancient Tea-Horse Trail

    Institute of Scientific and Technical Information of China (English)

    ZHANGHONG

    2005-01-01

    FOR centuries, Southwest China's Yunnan Province has been known as home of black tea. It was in the Tang Dynasty(618 - 907) that caravans began to transport bricks of tea from Xishuangbanna and Simao to India, passing through Yunnan's Dali and Lijiang and Tibet. The route became known as the Ancient Tea-Horse Trail.

  10. Effects of Aerobic Endurance Training and Tea Polyphenols on the Anti-oxidative Capability of the Liver Cells of Fat-rich Diet Rats%有氧运动结合茶多酚对高脂膳食大鼠肝脏抗氧化能力的影响

    Institute of Scientific and Technical Information of China (English)

    罗艳蕊; 王建设; 李新; 季浏

    2011-01-01

    目的:观察有氧耐力运动及茶多酚补充对高脂膳食大鼠肝脏抗氧化能力的影响。方法:雄性SD大鼠48只,随机分为6组:安静对照组、高脂膳食组、运动对照组、高脂膳食运动组、高脂膳食低茶多酚运动组、高脂膳食高茶多酚运动组。检测大鼠肝脏T-AOC、SOD、CAT、NOS活性及MDA、NO含量。结果:高脂膳食大鼠肝脏SOD、CAT活性及MDA含量显著高于对照组;高脂膳食运动组SOD、CAT活性较高脂膳食组显著上升,MDA含量显著降低;高脂膳食茶多酚运动组SOD活性升高、CAT活性下降,MDA恢复至安静对照组水平。高脂膳食大鼠肝脏TNOS、iNOS活性及NO含量都比安静对照组显著增加;高脂膳食运动组三者较高脂膳食组显著下降。与安静对照组比较,其它5组T-AOC均显著提高,其中茶多酚补充与运动联合作用提高幅度最大。结论:有氧耐力运动可以提高高脂膳食大鼠肝脏抗氧化能力,有氧耐力运动联合茶多酚补充对高脂膳食大鼠肝脏抗氧化能力的提高效果优于单纯的有氧耐力运动。%Objective: To observe the influence of aerobic endurance exercise and tea polyphenols on the liver of fat-rich diet rat's metabolize of reactive oxygen species(ROS) and reactive nitrogen species(RNS).Methods: 48 healthy male SD rats were randomly divided into six groups: Group A(quietness control group),Group B (fat-rich diet group),Group C(exercise control group),Group D(exercise+fat-rich diet group),Group E(exercise + fat-rich diet + low tea polyphenols group),Group F(exercise + fat-rich diet and high tea polyphenols group).The authors detected the activity of total anti-oxidative capacity(T-AOC),super oxide dimutese(SOD),catalase(CAT),total nitric oxide synthase(TNOS),inducible nitric oxide synthase(iNOS) and the content of malonaldehyde(MDA),nitric oxide(NO) in the rat's liver

  11. Transformation of tea catechins and flavonoid glycosides by treatment with Japanese post-fermented tea acetone powder

    OpenAIRE

    Tanaka, Takashi; Umeki, Hirotaka; NAGAI, SACHI; Shii, Takuya; Matsuo, Yosuke; Kouno, Isao

    2012-01-01

    Japanese post-fermented teas are produced by a combination of aerobic and anaerobic microbial fermentation of the leaves of tea plant. Recently, it was revealed that tea products contain characteristic polyphenols identical to the tea catechin metabolites produced by mammalian intestinal bacteria, such as (2S)-1-(3′,4′,5′-trihydroxyphenyl)-3-(2″,4″,6″-trihydroxyphenyl)-propan-2-ol (EGC-M1). In the present study, degradation of epigallocatechin-3-O-gallate (EGCg) and epigallocatechin (EGC) wit...

  12. GREEN TEA FESTIVAL

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ What is the green tea? The green tea belongs to the type of non-fermenting tea, with a quality feature of "clear tea infusion with green leaves"; this type of tea has the biggest output in China, and the basic processing procedure of the green tea is divided into three steps: heating, rubbing and drying. According to the different processing technologies, the green tea is divided into fried green tea, baked green tea, steamed green tea and dried green tea. The steamed green tea is to heat the tea by steaming; to heat the tea by pan-frying can be divided into frying, baking and drying, which is called heating by frying, heating by baking and heating by drying. West LakeLongjing, Xinyang Maojian, Bi Luochun, and Sanbeixiang belong to fried green tea; Mount Huang Maofeng, Youjiyuluo, and Luhai pekoe belong to baked green tea;Enshiyulu belongs to steamed green tea.

  13. PAH in tea and coffee

    DEFF Research Database (Denmark)

    Duedahl-Olesen, Lene; Navarantem, Marin; Adamska, Joanna;

    For food regulation in the European Union maximum limits on other foods than tea and coffee includes benzo[a]pyrene and the sum of PAH4 (sum of benzo[a]pyrene, chrysene, benz[a]anthracene and benzo[b]fluoranthene). This study includes analysis of the above mentioned PAH in both, tea leaves, coffee...... for accumulation of PAH in tea leaves. Different varieties of tea leaves were analyzed and highest concentrations were found in leaves from mate and black tea with maximum concentrations of 32 μg/kg for benzo[a]pyrene and 115 μg/kg for the sum of PAH4. Also, coffee beans are roasted during processing. However......, both benzo[a]pyrene and PAH4 concentrations were more than ten times lower for coffee beans than for tea leaves. Highest levels were found for PAH4 of solid instant coffee (5.1 μg/kg). Data were used to calculate the exposure of benzo[a]pyrene (15%) and sum of PAH4 (10%) from tea and coffee...

  14. Overview of Antibacterial, Antitoxin, Antiviral, and Antifungal Activities of Tea Flavonoids and Teas

    Science.gov (United States)

    Tea leaves produce secondary metabolites, organic compounds that are involved in the defense of the plants against invading pathogens including insects, bacteria, fungi, and viruses. These metabolites include polyphenolic compounds, the six so-called catechins, and the methyl-xanthine alkaloids caf...

  15. Study on the ability of bile salt-binding among different tea extracts in vitro%不同茶浸提液对胆酸盐的结合及其降血脂机理的研究

    Institute of Scientific and Technical Information of China (English)

    胡凯; 黄惠华

    2011-01-01

    The binding capacity of the extracts from green tea,Oolong tea,Puer tea,dark tea and black tea to bile salts were compared as well as the correlation between tea polyphenol and the bile salts binding capability in vitro under the conditions of the simulated human digestive environment.The results showed that the rate of bile salts-binding was fast and there was a significant correlation between the bile salts binding capacity and tea polyphenol content.Relative to cholestyrammine,green tea extract showed the 38.4% binding capacity for sodium taurocholate,46.5% for glycocholate and 42.0% for sodium cholate,exhibiting the maximum binding capability,followed by the semi-fermented(e.g.Oolong tea)and fully fermented teas(e.g.Puer tea,black tea and dark tea).%在体外模拟人体消化环境,研究绿茶浸提液对胆酸盐的等温吸附性质,比较不同茶浸提液对胆酸盐的结合能力,同时探讨了茶多酚含量与茶浸提液结合胆酸盐的相关关系。结果表明,茶浸提液对胆酸盐结合速度较快,Freundilich等温式、Langmiur等温式均能良好地反映绿茶浸提液对胆酸盐的结合;绿茶浸提液结合胆酸盐的能力最强,其结合牛磺胆酸钠、甘氨胆酸钠、胆酸钠的量分别是考来烯胺的38.4%、46.5%和42.0%;其次是半发酵茶(铁观音,35.2%、41.7%和42.6%)、发酵茶(普洱茶、滇红茶和六堡茶)。不同茶浸

  16. Chapter 8. Tea and Cancer Prevention: Epidemiological Studies

    OpenAIRE

    Yuan, Jian-Min; Sun, Canlan; Butler, Lesley M.

    2011-01-01

    Experimental studies have consistently shown the inhibitory activities of tea extracts on tumorigenesis in multiple model systems. Epidemiologic studies, however, have produced inconclusive results in humans. A comprehensive review was conducted to assess the current knowledge on tea consumption and risk of cancers in humans. In general, consumption of black tea was not associated with lower risk of cancer. High intake of green tea was consistently associated with reduced risk of upper gastro...

  17. Effect of Chemical Composition of Honey on Cream Formation in Honey Lemon Tea

    Directory of Open Access Journals (Sweden)

    J. Hategekimana

    2011-02-01

    Full Text Available The aim of this study was to assess the effect of chemical composition of honey and environmental factors such as pH, storage temperature and ionic strength on cream formation in honey lemon tea drink. The results showed that minerals, Ca2+ and Mg2+ were involved in cream formation while Fe2+ participated in changing color to tar black at pH 4.12 and above. Honey rich in polyphenols and proteins increased the cream to 2.51%. High proline content (0.506 mg/g induced creaming in GH (Germany Honey infusion while Ca2+ and Mg2+ were responsible for creaming in CH (Chinese Honey infusion. The higher the amount of the total solids in honey, the higher the amount of cream formed. Honey lemon tea creaming can be reduced by controlling interaction between proteins and polyphenols, by pre-treatment of honey (i.e., to remove colloidal matters or by removing Ca2+ or Mg2+.

  18. Estimated Dietary Polyphenol Intake and Major Food and Beverage Sources among Elderly Japanese

    Directory of Open Access Journals (Sweden)

    Chie Taguchi

    2015-12-01

    Full Text Available Estimating polyphenol intake contributes to the understanding of polyphenols’ health benefits. However, information about human polyphenol intake is scarce, especially in the elderly. This study aimed to estimate the dietary intake and major sources of polyphenols and to determine whether there is any relationship between polyphenol intake and micronutrient intake in healthy elderly Japanese. First, 610 subjects (569 men, 41 women; aged 67.3 ± 6.1 years completed food frequency questionnaires. We then calculated their total polyphenol intake using our polyphenol content database. Their average total polyphenol intake was 1492 ± 665 mg/day, the greatest part of which was provided by beverages (79.1%. The daily polyphenol intake differed largely among individuals (183–4854 mg/day, also attributable mostly to beverage consumption. Coffee (43.2% and green tea (26.6% were the major sources of total polyphenol; the top 20 food items accounted for >90%. The polyphenol intake did not strongly correlate with the intake of any micronutrient, suggesting that polyphenols may exert health benefits independently of nutritional intake. The polyphenol intake in this elderly population was slightly higher than previous data in Japanese adults, and beverages such as coffee and green tea contributed highly to the intake.

  19. Sonme Factors that Affect the Free Radical-scavenging Activity of Tea Extracts

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Some factors that affect the free radical-scavenging activety of two tea extracts were studied in vitro. It was found that concentration of tea extract or heating tea extract or treating with activated carbon and diatomite all had obvious effect on the scavenging activety of green tea extract ,but heating or treating with diaomite had less effect on the scavenging activity of black tea extract. Ascorbic acid, for having synergic effect with tea extracts, could enhance the scavenging activity of tea extracts markedly, and the contrary was cupric ion. Reducing sugars such as fructose and glucose also had some syncrgic effect to tea extracts.

  20. THE EFFECTS OF TEA WASTES ON SOIL ENZYME ACTIVITY AND NITRIFICATION

    OpenAIRE

    ARCAK, Sevinç; A. Cihat KÜTÜK; Koray HAKTANIR; Gökhan ÇAYCI

    1997-01-01

    The research was carried out on tea wastes supplied from factories of General Directorate of Tea Enterprises. The aim of this study is to determine the effects of composted, enriched composted tea wastes along with coarse and fine tea wastes released by tea factories processing raw tea leaves into black tea, on soil enzyme activity and nitrification. Tea wastes were applied to pots of 200 g-soil capacity, at the rate of 0 %, 2.5 % and 5.0 % of the pot capacity in addition, 200 µg/g N, in the...

  1. A Simple Method for Isolation of Caffeine from Black Tea Leaves: Use of a Dichloromethane-Alkaline Water Mixture as an Extractant

    Science.gov (United States)

    Onami, Tetsuo; Kanazawa, Hitoshi

    1996-06-01

    A simple procedure for the isolation of caffeine from tea leaves has been established without using hot or boiling water. A mixture of tea leaves, dichloromethane, and 0.2 M NaOH was shaken for 7 min, and the organic layer was separated. After evaporation of the organic solvent, residual crystals were purified by recrystallization to give 20-30 mg (student yield) of pure caffeine from one tea bag (2 g).

  2. Effects of green tea catechins on gramicidin channel function and inferred changes in bilayer properties

    NARCIS (Netherlands)

    Ingólfsson, Helgi I; Koeppe, Roger E; Andersen, Olaf S

    2011-01-01

    Green tea's health benefits have been attributed to its major polyphenols, the catechins: (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG), (-)-epigallocatechin (EGC), and epicatechin (EC). Catechins (especially EGCG) modulate a wide range of biologically important molecules, inclu

  3. Effects of plant polyphenols and a-tocopherol on lipid oxidation, microbiological characteristics, and biogenic amines formation in dry-cured bacons

    Science.gov (United States)

    Effects of plant polyphenols (tea polyphenol, grape seed extract, and gingerol) and a-tocopherol on physicochemical parameters, microbiological counts, and biogenic amines were determined in dry-cured bacons at the end of ripening. Results showed that plant polyphenols and a-tocopherol significantly...

  4. 茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制%Anti-obesity effects and its mechanism of tea polyphenols on obesity rats induced by olanzapine

    Institute of Scientific and Technical Information of China (English)

    钟满群; 唐言利; 吴铎; 吕俊华

    2011-01-01

    AIM To study the anti-obesity effects of tea polyphenols (TP) on olanzapine induced weight gain in rats and its mechanism. METHODS Fifty female SD rats were randomized into 2 groups, 10 rats in the normal control group and 40 rats in the obesity model group. The obesity model rats received olanzapine (1.2 mg-kg"1-d"1) daily via gavage 1 h before the beginning of the dark-phase for 2 weeks, while the rats in the normal control group received an equivalent volume of distilled water. At the beginning of wk 3, the obesity model rats were randomized into 4 groups: model group (distilled water) , sibutramine (2.5 mg ·kg-1·d-1) group, low-dose (150 mg·kg-1·d-1) group and high-dose (300 mg·kg-1·d-1) group of TP, 10 rats in each group, and the rats were administered with olanzapine continuously. TP and sibutramine were given by gavage for 5 weeks. The body weight and fat weight around the kidney and uterus were measured, and Lee' s index and fat coefficient were calculated. The levels of serum leptin and adiponectin ( ADP) , superoxide dismutase (SOD) activity and malondialchehyche (MDA) content were tested respectively. RESULTS Compared with the normal control group, the body weight, fat weight, Lee' s index, fat coefficient, the level of serum leptin were significantly increased and serum ADP decreased significantly in the model group (P 0.05). CONCLUSION TP can reduce olanzapine induced weight gain in rats, which may be associated with the enhanced regulation of ADP and antioxidative ability.%目的 探讨茶多酚对奥氮平诱导肥胖大鼠的减肥作用及其机制.方法 SD雌性大鼠50只,随机分为正常对照组(10只)和奥氮平诱导肥胖模型组(40只).肥胖模型组于黑暗时相前1 h奥氮平1.2 mg·kg-1·d-1灌胃,正常对照组给予等容量蒸馏水,连续2 wk.于wk 3开始,再将肥胖模型大鼠随机分为4组:模型组、西布曲明(2.5 mg·kg-1·d-1)组、茶多酚低(150 mg·kg-1·d-1)剂量组和茶多酚高(300 mg·kg-1·d-1)

  5. Alleviative effects of green tea polyphenols on cyclosporine A-induced inhibition of vasorelaxation%绿茶多酚可抑制环孢素A所致的血管舒张作用

    Institute of Scientific and Technical Information of China (English)

    高文波; 姚许平; 姜继光

    2011-01-01

    BACKGROUND: Vasorelaxation plays an important role In the occurrence of cyclosporine A (CsA)-induced nephrotoxlcity.OBJECTIVE: To observe the alleviative effects of green tea polyphenols (GTP) on CsA-induced inhibition of vasorelaxation and the underlying mechanisms.METHODS: Sprague-Dawley rats were randomly and evenly divided into four groups: CsA, control, CsA + GTP, and GTP. After 5 weeks of drug treatment, blood urea nitrogen (BUN) and creatinine (Cre) levels were determined. Then the thoracic aorta rings were mounted on a bath system, and acetylcholine was used to induce vasorelaxation. The effects of L-NAME and indomethacin and the denuded vasorelaxation were evaluated.RESULTS AND CONCLUSION: The BUN and Cre levels in the CsA group were significantly higher than those in the control group (P < 0.05). The maximal response (Emax%) for acetylcholine-induced vasorelaxation in the CsA group was significantly lower than that in the control and GTP groups. After pretreatment with L-NAME, vasorelaxation was significantly lower in the CsA,CsA+GTP and GTP groups than in the control group. After pretreatment with indomethacin, vasorelaxation was significantly higher in the control, CsA +GTP, and GTP groups than in the CsA group. The level of nitric oxide metabolites in the vascular tissue in the CsA group was significantly lower compared with other groups. The results demonstrated that CsA can decrease nitric oxide levels in vascular tissues and induce abnormal endothelium-dependent vasorelaxation, which is mediated by nitric oxide pathway.%背景:血管舒张在环孢素A所致肾毒性的发生过程中起重要的作用.目的:观察绿茶多酚对环孢素A抑制血管舒张的缓解作用及其机制.方法:SD大鼠随机平均分为4组:环孢素A组、对照组、环孢素A+绿茶多酚组和绿茶多酚组.经5周的药物处理之后,测定大鼠血尿素氮和肌酐水平.将各组大鼠的胸主动脉环放置于水浴系统上,以乙酰胆碱诱发血管舒

  6. A biochemical study on ameliorative effect of green tea (Camellia sinensis) extract against contrast media induced acute kidney injury

    OpenAIRE

    Nasri, Hamid; Ahmadi, Ali; Baradaran, Azar; Nasri, Parto; Hajian, Shabnam; Pour-Arian, Armita; Kohi, Golnoosh; Rafieian-Kopaei, Mahmoud

    2013-01-01

    Introduction: Reactive oxygen species have been shown to be mediators of kidney injury and green tea polyphenols are potent-free radical scavengers.Objectives: In this study we sought to examine whether green tea was able to protect renal toxicity induced by contrast media or not. Materials and Methods: In this experimental study 40 rats were randomly divided into four groups including: 1) control group 2) contrast media group 3) contrast media plus green tea 4) Green tea pretreatment and con...

  7. The antimicrobial possibilities of green tea

    Directory of Open Access Journals (Sweden)

    Wanda C Reygaert

    2014-08-01

    Full Text Available Green tea is a popular drink, especially in Asian countries, although its popularity continues to spread across the globe. The health benefits of green tea, derived from the leaves of the Camellia sinensis plant, have been studied for many years. Fairly recently, researchers have begun to look at the possibility of using green tea in antimicrobial therapy, and the potential prevention of infections. The particular properties of catechins found in the tea have shown promise for having antimicrobial effects. There are four main catechins (polyphenols found in green tea: (--epicatechin (EC, (--epicatechin-3-gallate (ECG, (--epigallocatechin (EGC, and (--epigallocatechin-3-gallate (EGCG. Three of these, ECG, EGC, and EGCG have been shown to have antimicrobial effects against a variety of organisms. These catechins have exhibited a variety of antimicrobial mechanisms. The results of studies on the antimicrobial effects of green tea have shown that the potential for preventive and therapeutic purposes is present. Further data collection on studies performed with human consumption during the course of infections, and studies on the occurrence of infections in populations that consume regular amounts of green tea will be necessary to complete the picture of its antimicrobial possibilities.

  8. The effects of tea polyphenol on the oxidative stress and cellular apoptosis of testic-ular tissues in rat varicocele%茶多酚对精索静脉曲张大鼠睾丸组织氧化应激和细胞凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    柯鑫文; 武政华; 冯少勇; 张利; 武进峰; 程伟; 成建军; 张建东; 张雁钢

    2015-01-01

    目的 通过研究茶多酚对精索静脉曲张大鼠睾丸组织氧化应激和细胞凋亡的影响 ,探讨茶多酚是否对精索静脉曲张大鼠生精功能具有保护作用 ,为临床上应用茶多酚治疗精索静脉曲张提供理论依据和实验基础.方法 32只雄性青春期Wistar大鼠随机分为4组 ,每组8只.其中第Ⅰ组为假手术组(仅游离左肾静脉 ,不结扎);第Ⅱ组为模型组(建立精索静脉曲张模型);第Ⅲ 、Ⅳ组为茶多酚干预组(先建立精索静脉曲张模型 ,然后分别给予不同浓度的茶多酚干预).造模4周后 ,第Ⅰ组及第Ⅱ组均给予生理盐水1 mL/100 g ,第Ⅲ组给予茶多酚10 mg/kg [即 :(1 mL盐水+1 mg茶多酚)/100 g ] ,第Ⅳ组给予茶多酚40 mg/kg [即 :(1 mL盐水+4 mg茶多酚)/100 g];1次/d ,灌胃 ,持续4周.4周后4组大鼠均处死取左侧睾丸组织 ,分别测定超氧化物歧化酶(SOD)活力、丙二醛(malondialdehyde ,MDA)含量及检测凋亡指数(apoptosis index ,AI).结果 第Ⅰ组SOD活力最高 ,第Ⅱ组低于第Ⅲ组和第Ⅳ组 ,差异有统计学意义(P<0 .05);第Ⅰ组MDA含量最低 ,第Ⅲ 、Ⅳ组低于第Ⅱ组 ,差异有统计学意义(P<0 .05);第Ⅰ组AI最低 ,第Ⅲ 、Ⅳ组AI低于第Ⅱ组 ,差异有统计学意义(P<0 .05).结论 茶多酚能显著降低精索静脉曲张大鼠睾丸组织的氧化应激和细胞凋亡 ,间接改善精索静脉曲张大鼠睾丸的生精功能.%Objective To explore the protective role of tea polyphenol on the oxidative stress and cellular apoptosis of testicular tissues in experimentally-induced varicocele rat models .Methods A total of 32 male prepubertal Wistar rats were randomly divided into 4 groups ,with 8 rats in each group .Group I was the sham operation group .Group Ⅱ was the varicocele model group .Group Ⅲ and Ⅳ were the tea polyphenol groups ,in which the varicocele models were treated with tea polyphe-nol of different concentrations .Four weeks after the models were

  9. Studies on the Effect of Processing Methods on the Antihyperglycemic activity of Herbal Teas from Leaves of Vernonia amygdalina Del

    OpenAIRE

    G I Okafor; C O Okoli; A S Odo; N R Kelechi

    2009-01-01

    The leaves of Vernonia amygdalina Del (Asteraceae) was processed into herbal tea using the black and green tea processing techniques. The green tea obtained was used as such while a portion of the black tea was flavored with O. basilicum or O. gratissimum. Methanol extracts of the flavored and unflavored herbal teas, obtained by cold maceration, were subjected to pharmacological studies for antihyperglycemic activity in alloxan diabetic rats and pytochemical analysis. Results showed that sing...

  10. Protective effects of Mengshan green tea and hawk tea against UV-ray irradiation

    International Nuclear Information System (INIS)

    A group of cultured normal human skin-derived fibroblasts was used as the cell model to investigate protective and repair effects of aqueous extracts of Mengshan green tea and Hawk tea against 320-400 nm UV-ray irradiation, with the methods of MTT colorimetry and LDH release. It was found that the aqueous extracts had strong protective effect on fibroblasts against the UV-rays with dose dependence. There were no significant differences between the two kinds of tea aqueous extracts in a higher concentration of 5 mg/mL, whereas at lower concentrations of 2.5 and 1.25 mg/mL the, green tea aqueous extract was less effective than the hawk-tea aqueous extract in protecting fibroblasts from the UV-ray damage. Meanwhile, it was discovered that the green tea and hawk-tea aqueous extract could repair damages induced by the UV irradiation with dose dependence. But there were no statistically significant differences between the two kinds of aqueous extract. The effects may be related to antioxidant effect of tea polyphenol. (authors)

  11. Polyphenols: skin photoprotection and inhibition of photocarcinogenesis.

    Science.gov (United States)

    Afaq, F; Katiyar, S K

    2011-12-01

    Polyphenols are a large family of naturally occurring plant products and are widely distributed in plant foods, such as, fruits, vegetables, nuts, flowers, bark and seeds, etc. These polyphenols contribute to the beneficial health effects of dietary products. Clinical and epidemiological studies suggest that exposure of the skin to environmental factors/pollutants, such as solar ultraviolet (UV) radiation induce harmful effects and leads to various skin diseases including the risk of melanoma and non-melanoma skin cancers. The incidence of non-melanoma skin cancer, comprising of squamous cell carcinoma and basal cell carcinoma, is a significant public health concern world-wide. Exposure of the skin to solar UV radiation results in inflammation, oxidative stress, DNA damage, dysregulation of cellular signaling pathways and immunosuppression thereby resulting in skin cancer. The regular intake of natural plant products, especially polyphenols, which are widely present in fruits, vegetables, dry legumes and beverages have gained considerable attention as protective agents against the adverse effects of UV radiation. In this article, we first discussed the impact of polyphenols on human health based on their structure-activity relationship and bioavailability. We then discussed in detail the photoprotective effects of some selected polyphenols on UV-induced skin inflammation, proliferation, immunosuppression, DNA damage and dysregulation of important cellular signaling pathways and their implications in skin cancer management. The selected polyphenols include: green tea polyphenols, pomegranate fruit extract, grape seed proanthocyanidins, resveratrol, silymarin, genistein and delphinidin. The new information on the mechanisms of action of these polyphenols supports their potential use in skin photoprotection and prevention of photocarcinogenesis in humans. PMID:22070679

  12. Research progress in white tea and Anji white tea%白茶与安吉白茶的研究进展

    Institute of Scientific and Technical Information of China (English)

    邓静; 王远兴; 丁建

    2013-01-01

    在中国六大茶(绿茶、红茶、乌龙茶、白茶、黄茶和黑茶)中,安吉白荼属于绿茶,由于名称与白茶相似,且具有类似的药理功效,常易被混淆本文从白茶和安吉白茶的发展历史、药理学研究和产品开发三个方面对白茶和安吉白荼进行综述,并对两者的发展前景进行展望.%White tea is one of China's six major teas (green tea,black tea,oolong tea,white tea,yellow tea and dark tea);Anji white tea,which has similar name and pharmacological effects with white tea,actually belongs to green tea. In this article,history of white tea and Anji white tea was briefly introduced,pharmacological effects and product development of white tea and Anji white tea were reviewed,and future prospects of research on white tea and Anji white tea were also proposed.

  13. Chemical Analysis and Antioxidant Activity in vitro of Polysaccharides Extracted from Lower Grade Green Tea

    OpenAIRE

    Ping Chen; Yongjun Zhang; Liyun Zhu; Hui Jin; Gaofan Zhang; Dongyang Su; Jia Li

    2013-01-01

    Tea is a well-known and important agricultural product in the world. The Crude Polysaccharides from tea leaves (CP) probably have good antioxidant activities. However, whether or not the antioxidant abilities of CP depend on tea polyphenols in the CP is not understanded. In this study, four CP fractions (TPF30, TPF50, TPF70 and TPF90) were isolated from CP and their antioxidant activities were compared. Meanwhile, Chemical and physical characteristics of CP and four CP fractions were investig...

  14. Periodontal Dressing-containing Green Tea Epigallocathechin gallate Increases Fibroblasts Number in Gingival Artifical Wound Model

    OpenAIRE

    Ardisa U. Pradita; Agung P. Dhartono; Catur A. Ramadhany; Ali Taqwim

    2014-01-01

    Green tea leaf (Camellia sinensis) is one of herbal plants that is used for traditional medicine. Epigallocatechin gallate (EGCG) in green tea is the most potential polyphenol component and has the strongest biological activity. It is known that EGCG has potential effect on wound healing. Objective: This study aimed to determine the effect of adding green tea EGCG into periodontal dressing on the number of fibroblasts after gingival artificial wound in animal model. Methods: Gingival artific...

  15. Green tea intake is associated with urinary estrogen profiles in Japanese-American women

    Directory of Open Access Journals (Sweden)

    Fuhrman Barbara J

    2013-02-01

    Full Text Available Abstract Scope Intake of green tea may reduce the risk of breast cancer; polyphenols in this drink can influence enzymes that metabolize estrogens, known causal factors in breast cancer etiology. Methods and results We examined the associations of green tea intake ( Conclusions Findings suggest that intake of green tea may modify estrogen metabolism or conjugation and in this way may influence breast cancer risk.

  16. Daily Fluoride Intake from Iranian Green Tea: Evaluation of Various Flavorings on Fluoride Release

    OpenAIRE

    Afshin Maleki; Hiua Daraei; Elham Mohammadi; Shiva Zandi; Pari Teymouri; Amir Hossien Mahvi; Fardin Gharibi

    2016-01-01

    With increased awareness of the health benefits of the compounds in green tea, especially polyphenols, its consumption is rising. The main purpose of this study is to determine the effect of different additives on the released fluoride into tea liquor and also daily fluoride intake. The concentrations of fluoride, nitrate, sulfate, and chloride were measured in 15 different flavored green teas (Refah-Lahijan). The fluoride and other anion concentrations were measured by ion chromatography met...

  17. Determination of As,Hg and Pb in tea polyphenols by hydride generation atomic fluorescence spectrome-try%氢化物发生-原子荧光光谱法测定茶多酚中的As、Hg和Pb

    Institute of Scientific and Technical Information of China (English)

    刘海涛

    2015-01-01

    建立了氢化物发生原子荧光光谱法测定茶多酚中微量As、Hg和Pb的方法。采用 HNO3-HClO4湿法消解样品,研究酸度、KBH4浓度、载气流速、基体改进剂等条件对原子荧光信号强度的影响。测定结果显示, As、Hg和Pb的检出限(3 s)分别为0·39、0·037和0·78 mg·L -1,相对标准偏差分别为1·08、2·56和2·93%(n=11),加标回收率均为80~105%。综上,该方法具有操作简便、快速、灵敏度高等优点,可用于茶多酚中微量 A s、Hg和Pb的测定。%A method for determination of As 、Hg and Pb in tea polyphenols by HG‐AFS was proposed. Polyphenols sample was diguested by HNO3‐HClO4. Operation conditions such as acidity ,KBH4 concen‐tration ,carrier gas flow rate and matrix modifier were investigated. Detection limit(3 s) are 0.38 ,0.037 and 0.78 mg・L -1 for As ,Hg and Pb ,respectively. Relative standard deviations (n= 11) are 1.08% , 2.56% and 2.93% . Results of the method were proved by the recovery of standard addition of the real samples(80%‐105% ).

  18. Influence on longevity of blueberry, cinnamon, green and black tea, pomegranate, sesame, curcumin, morin, pycnogenol, quercetin, and taxifolin fed iso-calorically to long-lived, F1 hybrid mice.

    Science.gov (United States)

    Spindler, Stephen R; Mote, Patricia L; Flegal, James M; Teter, Bruce

    2013-04-01

    Phytonutrients reportedly extend the life span of Caenorhabditis elegans, Drosophila, and mice. We tested extracts of blueberry, pomegranate, green and black tea, cinnamon, sesame, and French maritime pine bark (Pycnogenol and taxifolin), as well as curcumin, morin, and quercetin for their effects on the life span of mice. While many of these phytonutrients reportedly extend the life span of model organisms, we found no significant effect on the life span of male F1 hybrid mice, even though the dosages used reportedly produce defined therapeutic end points in mice. The compounds were fed beginning at 12 months of age. The control and treatment groups were iso-caloric with respect to one another. A 40% calorically restricted and other groups not reported here did experience life span extension. Body weights were un-changed relative to controls for all but two supplemented groups, indicating most supplements did not change energy absorption or utilization. Tea extracts with morin decreased weight, whereas quercetin, taxifolin, and Pycnogenol together increased weight. These changes may be due to altered locomotion or fatty acid biosynthesis. Published reports of murine life span extension using curcumin or tea components may have resulted from induced caloric restriction. Together, our results do not support the idea that isolated phytonutrient anti-oxidants and anti-inflammatories are potential longevity therapeutics, even though consumption of whole fruits and vegetables is associated with enhanced health span and life span. PMID:23432089

  19. Xerophilic mycopopulations of teas in bulk

    Directory of Open Access Journals (Sweden)

    Škrinjar Marija M.

    2011-01-01

    Full Text Available d.o.o., Novi Sad AU Krunić Vesna J. AF EKOLd.o.o., Novi Sad KW teas % mould contamination % thermal treatment KR nema Other the water, tea is the most popular beverage in the world today. They are used for ages, in the beginning as refreshing drinks, and later more for their healing properties. Teas have been demonstrated to show antioxidative, anti-carcinogenic, and anti-microbial properties. Considering that the teas, during the production, are not treated with any temperature, there is high risk for contamination with different type of microorganisms, especially with moulds. Moulds are ubiquitously distributed in nature and their spores can be found in the atmosphere even at high altitudes and under favorable conditions of temperature and humidity, moulds grow on many commodities including cereals, oil seeds, nuts, herbs and spices. Most of them are potential producers of mycotoxins which present a real hazard to human health. The aim of this work was to investigate total mould count and to identify moulds isolated from teas in bulk, than from teas treated with hot, sterile, distilled water and from the tea filtrates. Tested teas were peppermint, sage, yarrow, black tea, bearberry, lemon balm, mixture of teas from Zlatibor. In teas in balk was observed high contamination with different kinds of moulds (1.84-4.55 cfu/g, such as Aspergillus awamori, A. lovaniensis, A niger, A. phoenicus, A. repens, A. restrictus, A. sydowii, A. versicolor, Eurotium amstelodami, E. chevalieri, E. herbariorum, Penicillium chrysogenum, and Scopulariopsis brevicaulis. The most frequent were species from Aspergillus and Eurotium genera. Thermal treatment with hot, sterile, distilled water reduced the number of fungal colonies. Aspergillus awamori was the most resistant and appeared in six samples of filtrates of tea, Aspergillus niger in one sample and Penicillium chrysogenum in one sample.

  20. Camellia sinensis (Tea: Implications and role in preventing dental decay

    Directory of Open Access Journals (Sweden)

    Puneet Goenka

    2013-01-01

    Full Text Available Tea is one of the most commonly consumed beverages with bioactive compounds like polyphenols-flavonoids-catechins, which are thought to be responsible for the health benefits that have traditionally been attributed to the tea. These compounds have multi-dimensional effects such as antibacterial action, inhibitory action on the bacterial and salivary amylase and inhibition of acid production. This article outlines the possible role of these compounds coupled with the presence of high fluoride content in tea to exhibit an anticariogenic effect.

  1. Camellia sinensis (Tea): Implications and role in preventing dental decay.

    Science.gov (United States)

    Goenka, Puneet; Sarawgi, Aditi; Karun, Vinayak; Nigam, Anant G; Dutta, Samir; Marwah, Nikhil

    2013-07-01

    Tea is one of the most commonly consumed beverages with bioactive compounds like polyphenols-flavonoids-catechins, which are thought to be responsible for the health benefits that have traditionally been attributed to the tea. These compounds have multi-dimensional effects such as antibacterial action, inhibitory action on the bacterial and salivary amylase and inhibition of acid production. This article outlines the possible role of these compounds coupled with the presence of high fluoride content in tea to exhibit an anticariogenic effect. PMID:24347923

  2. Extraction of Catechins from Green Tea Using Ultrasound

    Science.gov (United States)

    Koiwai, Hitoshi; Masuzawa, Nobuyoshi

    2007-07-01

    Recently, hazardous properties of synthetic substances contained in foods and medicines have been observed. Therefore, the pharmacological actions of natural substances became the focus of attention. Catechins, which are polyphenols, are abundant in green tea. To extract green tea catechins, there are two types of method: extraction using hot water and organic solvents. In the extraction using hot water, green tea quality deteriorates and catechins are destroyed due to the heat applied. Accordingly, the extraction of catechins at low temperatures is appropriate, although the efficiency is low. Therefore, we attempted to increase the amount of catechins extracted with ultrasonic irradiation.

  3. Tea beverage in chemoprevention and chemotherapy of prostate cancer

    Institute of Scientific and Technical Information of China (English)

    Imtiaz A SIDDIQUI; Mohammad SALEEM; Vaqar M ADHAMI; Mohammad ASIM; Hasan MUKHTAR

    2007-01-01

    Prostate cancer (Pca) is the most frequently diagnosed malignancy and the sec-ond leading cause of cancer-related deaths in American males with similar trends in many western countries. The existing treatment approaches and surgical inter-vention have not been able to effectively cope with this dreaded disease. For these reasons, it is necessary to intensify our efforts for a better understanding of the disease process and for the development of novel approaches for its preven-tion and treatment. Based on considerable evidence from in vivo and in vitro data and epidemiological studies, in recent years the beverage tea has gained consid-erable attention for reducing the risk of several cancers. Much of the cancerpreventive effects of tea, especially green tea appear to be mediated by the polyphe-nols present therein. Geographical evidence suggests that the incidence and occurrence of Pca is lower in populations that consume tea regularly. This evi-dence suggests that tea polyphenols could be extrapolated to optimize their chemopreventive properties against Pca. Pca represents an excellent candidate disease for chemoprevention because it is typically diagnosed in men over 50 years of age and therefore, even a modest delay in neoplastic development achieved through pharmacological or nutritional intervention could result in a substantial reduction in the incidence of clinically detectable disease. In this review we address the issue of possible use of tea, especially green tea, for the prevention aswell as treatment of Pca.

  4. Reducing of radiation hazard by green tea

    International Nuclear Information System (INIS)

    Green tea extract with its high content of polyphenols, a class of bioflavonoids, makes it one of the most powerful natural antioxidant known. The present study was conducted to evaluate the antioxidant and protective roles of drinking green tea one week before the exposure of adult male albino rats to whole body gamma irradiation (6.0 Gy). The results revealed a significant decrease in final body weight, serum albumin, A/G ratio due to radiation exposure. On the other hand, mortality percentage, serum globulin, urea, cholesterol, triglycerides, alkaline phosphatase, alanine transferase (ALT) and aspartate transferase (AST) were significantly increased. The same effect of y irradiation appeared on liver and kidney under histologically investigation. Drinking of green tea before irradiation exerted noticeable amelioration of radiation damage

  5. Altering the phenolics profile of a green tea leaves extract using exogenous oxidases.

    Science.gov (United States)

    Verloop, Annewieke J W; Gruppen, Harry; Bisschop, Robbin; Vincken, Jean-Paul

    2016-04-01

    Transformation from green tea leaves into black tea involves oxidation of catechins into theaflavins and other complex phenolics by endogenous enzymes in tea leaves. By employing tyrosinase and laccase, both from Agaricus bisporus, on green tea catechins, the oxidation process was directed towards a higher theaflavins content, which is considered an important quality parameter in tea. The main tea catechins were incubated with tyrosinase and laccase, and product formation was monitored by RP-UHPLC-PDA-ESI-MS. The kind of catechin, their substitution with a galloyl group, and the type of oxidase used were important factors determining theaflavin concentrations. In particular, incubation of epicatechin with epigallocatechin with tyrosinase gave a high, stable theaflavin content. In a green tea extract, tyrosinase increased the proportion of theaflavins by twofold compared to black tea. Laccase mainly formed insoluble complexes. Our results indicate that the phenolic profile of tea can be modulated by using commercially available exogenous oxidases. PMID:26593607

  6. Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals

    Directory of Open Access Journals (Sweden)

    T. Andzi Barhé

    2016-01-01

    Full Text Available The present study is part of the evaluation of extracts of Glycine max L. Merr and Hibiscus L. Sabdariffa as antioxidants. A comparative study was performed with extracts of yellow tea and commercial red wine, two foods known for their antioxidant activity. The method applied is free radical scavenging using the 1,1-diphenyl-2-picrylhydrazyl (DPPH°. The antioxidant properties were identified and measured by the determining the anti-radical activity reducing index, expressed in percentage % RSA (Radical Scavenger Activity, and by the determination of the colouring intensity (IC50. All results are compared to those of ascorbic acid as reference antioxidant. The results indicate the following order for the antioxidant power of the extracts tested. % RSA (tea > % RSA (Glycine max % > RSA (red wine % > RSA (Sabdariffa Hibiscus, and colouring intensities (IC50 ranging from 4.62 μM (ascorbic acid to 1.10 μM (Hibiscus sabdariffa correlated with their chemical structure and the content of phenolic compounds.

  7. Beneficial effects of cocoa, coffee, green tea, and garcinia complex supplement on diet induced obesity in rats

    OpenAIRE

    Huang, Chi-Chang; Tung, Yu-Tang; Huang, Wen-Ching; Chen, Yi-Ming; Hsu, Yi-Ju; Hsu, Mei-Chich

    2016-01-01

    Background Cocoa, coffee, green tea and garcinia contain large amounts of polyphenols. Polyphenols are well-known phytochemicals and found in plants, and have modulated physiological and molecular pathways that are involved in energy metabolism, adiposity, and obesity. Methods To evaluate the obesity-lowering effect of a combined extract (comprising cocoa, coffee, green tea and garcinia; CCGG) in high-energy diet (HED)-induced obese rats. Male Sprague Dawley rats (8 weeks old) were randomly d...

  8. Quantification of Tea Flavonoids by High Performance Liquid Chromatography

    Science.gov (United States)

    Freeman, Jessica D.; Niemeyer, Emily D.

    2008-01-01

    We have developed a laboratory experiment that uses high performance liquid chromatography (HPLC) to quantify flavonoid levels in a variety of commercial teas. Specifically, this experiment analyzes a group of flavonoids known as catechins, plant-derived polyphenolic compounds commonly found in many foods and beverages, including green and black…

  9. Purple Tea and Its Extract Suppress Diet-induced Fat Accumulation in Mice and Human Subjects by Inhibiting Fat Absorption and Enhancing Hepatic Carnitine Palmitoyltransferase Expression

    OpenAIRE

    Shimoda, Hiroshi; Hitoe, Shoketsu; Nakamura, Seikou; Matsuda, Hisashi

    2015-01-01

    A number of clinical trials have been completed using green tea and black tea to investigate their effect in controlling weight in overweight adults. The results of these investigations, however, have often been contradictory, with some trials reporting positive effects of tea supplementation and some trials reporting no effect. As a result, the use of these teas for weight loss is controversial. Purple tea is a variety of green tea developed in Kenya (called TRFK306), which in addition to ce...

  10. Theagalloflavic Acid, a New Pigment Derived from Hexahydroxydiphenoyl Group, and Lignan Oxidation Products Produced by Aerobic Microbial Fermentation of Green Tea.

    Science.gov (United States)

    Matsuo, Yosuke; Matsuda, Tomoko; Sugihara, Keisuke; Saito, Yoshinori; Zhang, Ying-Jun; Yang, Chong-Ren; Tanaka, Takashi

    2016-01-01

    Chinese ripe pu-erh tea is produced by aerobic microbial fermentation of green tea. To clarify the microbial degradation of tea polyphenols, Japanese commercial green tea was mixed with Chinese ripe pu-erh tea, which retains microorganisms, and fermented for 5 d. Chromatographic separation yielded a novel water-soluble yellow pigment termed theagalloflavic acid. Spectroscopic and chemical evidence suggested that this pigment was produced by oxidative ring cleavage of hexahydroxydiphenoyl esters. In addition, two new oxygenated lignin metabolites, (+)-5,5'-dihydroxypinoresinol and 5-hydroxydihydrodehydrodiconiferyl alcohol, were also isolated together with known degradation products of quercetin and tea catechins. PMID:27373646

  11. Suppression of tumorigenesis in the Apcmin mouse: down-regulation of β-catenin signaling by a combination of tea plus sulindac

    OpenAIRE

    Orner, Gayle A.; Dashwood, W.-Mohaiza; Blum, Carmen A.; Díaz, G. Darío; Li, Qingjie; Dashwood, Roderick H.

    2003-01-01

    Epidemiological and animal studies suggest that tea may be protective towards cancers of the GI tract. White tea, the least processed form of tea, contains high levels of polyphenols and, like green tea, is chemopreventive towards heterocyclic amine-initiated colonic aberrant crypt formation in male F344 rats. We examined for the first time the relative effectiveness of white and green tea in suppressing intestinal tumorigenesis in C57BL/6J-ApcMin/+ (Apcmin) mice. Each tea was also compared w...

  12. Effect of Cocoa and Green Tea Consumption on Glucoregulatory Biomarkers in Insulin-Resistant Men and Women.

    Science.gov (United States)

    In vivo and in vitro research has shown that tea and cocoa consumption may have an impact on the glucoregulatory system. This pilot study investigated if there was a dose-response effect of cocoa procyanidins (CP) on glucoregulatory biomarkers and compared the effect of cocoa and tea polyphenols on ...

  13. Anticancer Efficacy of Polyphenols and Their Combinations

    Directory of Open Access Journals (Sweden)

    Aleksandra Niedzwiecki

    2016-09-01

    Full Text Available Polyphenols, found abundantly in plants, display many anticarcinogenic properties including their inhibitory effects on cancer cell proliferation, tumor growth, angiogenesis, metastasis, and inflammation as well as inducing apoptosis. In addition, they can modulate immune system response and protect normal cells against free radicals damage. Most investigations on anticancer mechanisms of polyphenols were conducted with individual compounds. However, several studies, including ours, have indicated that anti-cancer efficacy and scope of action can be further enhanced by combining them synergistically with chemically similar or different compounds. While most studies investigated the anti-cancer effects of combinations of two or three compounds, we used more comprehensive mixtures of specific polyphenols and mixtures of polyphenols with vitamins, amino acids and other micronutrients. The mixture containing quercetin, curcumin, green tea, cruciferex, and resveratrol (PB demonstrated significant inhibition of the growth of Fanconi anemia head and neck squamous cell carcinoma and dose-dependent inhibition of cell proliferation, matrix metalloproteinase (MMP-2 and -9 secretion, cell migration and invasion through Matrigel. PB was found effective in inhibition of fibrosarcoma HT-1080 and melanoma A2058 cell proliferation, MMP-2 and -9 expression, invasion through Matrigel and inducing apoptosis, important parameters for cancer prevention. A combination of polyphenols (quercetin and green tea extract with vitamin C, amino acids and other micronutrients (EPQ demonstrated significant suppression of ovarian cancer ES-2 xenograft tumor growth and suppression of ovarian tumor growth and lung metastasis from IP injection of ovarian cancer A-2780 cells. The EPQ mixture without quercetin (NM also has shown potent anticancer activity in vivo and in vitro in a few dozen cancer cell lines by inhibiting tumor growth and metastasis, MMP-2 and -9 secretion, invasion

  14. DETERMINATION OF FLAVONOIDS AND ANTIOXIDANT ACTIVITY IN LAPHET LABORATORY PROCESS AND TEA (CAMELLIA SINENSIS) PRODUCTS BETWEEN CHINA AND MYANMAR

    OpenAIRE

    Pyie Phyo Maung; Qian He

    2013-01-01

    In this study, the effect of extraction method on the flavonoids and antioxidant activity was investigated. The flavonoid contents of Laphet(fermented pickled tea leaves) possessed more than twice as much than fresh tea leaves. Chinese green tea had higher flavonoid contents than Myanmar green tea. But Myanmar black tea’s flavonoid contents were higher. In laphet, both countries tea’s products possessed similar contents. Antioxidant activity of tea extracts were determined by using DPPH assay...

  15. Effect of Black Tea Extract on Pork Quality and Biochemical Indicators%红茶提取物对猪肉品质及生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    李勇

    2011-01-01

    [目的]研究红茶提取物对猪肉品质及生化指标的影响.[方法]选60头50 kg左右的杜长大肥育猪,随机分为时照组和试验组,分别饲喂基础日粮和基础日粮+2 g/kg红茶提取物.至猪体重达100 kg左右时,测猪肉的各项指标.[结果]试验组猪的屠宰性能提高,屠宰率显著提高(P<0.05);猪肉品质得到显著改善,肉色显著改善(P<0.05),肌肉丰度极显著提高(P<0.01);生化指标得到改善,肌苷酸显著提高(P<0.05),胆固醇显著下降(P<0.05),铁、锌含量显著提高(P<0.05).[结论]饲料中添加2 g/kg红茶提取物可改善猪肉品质.%[ Objective ] To study the effect of black tea extract on pork quality and biochemical indicators. [ Method ] 60 DLY pigs weighed about 50 kg were selected and divided randomly into control group and experimental group. The control group was fed by basal diet, and the experimental group was fed by basal diet plus 2 g/kg black tea extract. Each indicators of the pork were detected when the pigs grew into 100 kg (s) weight. [ Result ] Slaughter performance of pigs in experimental group was improved, the slaughter rate increased significandy ( P < 0. 05 );The pork quality was improved significantly, pork color improved ( P < 0.05 ) , muscle abundance increased significantly ( P < 0. 01 ); Biochemical Indicators were improved, IMP increased significantly (P < 0. 05 ), cholesterol decreased significantly( P < 0. 05 ),iron and zinc content increased significantly(P <0.05). [ Conclusion] Diet added 2 g/kg black tea extract could improve pork quality.

  16. Kinetics Study on Black Tea Infusion: Effects of Super-comminuting Process and Temperature on the Extraction Rate of the Soluble Solid Contents%红茶汤动力学研究:超微粉碎工艺和温度对茶汤可溶性固形物成分萃取率的影响

    Institute of Scientific and Technical Information of China (English)

    高彦祥; 杨文雄

    2005-01-01

    本文研究了红茶叶和超微茶粉可溶性固形物含量的萃取动力学过程.不同温度(40~80℃)下茶汤的Brixs通过数字折射计测量,实验结果通过一级稳态模型加以解释,等级常数由Brixs随时间的增加率决定.结果表明:茶汤可溶性固形物含量随萃取温度的升高而增加,超微茶粉的等级常数是红茶叶的1.22~2.22倍.%The kinetics of extracting the soluble solid contents from black tea leaves in super-comminuted tea powder was studied.The Brixs of infusion was measured by digital refractometer at different temperatures from 40 to 80 ℃.The first order rate constant was determined from the rate of increase of the Brixs concentration in the tea infusion time and the result was interpreted by using a steady state model. The results showed that the first order rate constant of the super-comminuted tea powder was 1.27~2.22 times as large as that of black tea leaves, and that the Brixs increased when the extraction temperature increased.

  17. Plant polyphenols and oral health: old phytochemicals for new fields.

    Science.gov (United States)

    Varoni, E M; Lodi, G; Sardella, A; Carrassi, A; Iriti, M

    2012-01-01

    Despite the protective role of diets rich in fruit plant polyphenols against some cancers and chronic degenerative and inflammatory diseases, insufficient emphasis has been placed on oral health. Numerous studies have aimed to ascertain the role of polyphenols in the prevention and treatment of oral diseases; however, even when in vitro evidence appears convincing, the same is not true for in vivo studies, and thus there is a general paucity of solid evidence based on animal and clinical trials. To the best of our knowledge, only two reviews of polyphenols and oral health have been published; however, neither considered the potential role of whole plant extracts, which contain mixtures of many polyphenols that are often not completely identified. In the present study, our main aim was to review the current state of knowledge (search period: January 1965 to March 2011) on the effects of plant extracts/polyphenols on oral health. We found data on grapes, berries, tea, cocoa, coffee, myrtle, chamomile, honey/propolis, aloe extracts and the three main groups of polyphenols (stilbenes, flavonoids and proanthocyanidins). Their effects on caries, gingivitis, periodontal disease, candidiasis, oral aphtae, oral mucositis, oral lichen planus, leukoplakia and oral cancer were investigated. The data suggest that there is a lack of strong evidence, in particular regarding randomized clinical trials. However, a fascinating starting point has been provided by pre-clinical studies that have shown interesting activities of polyphenols against the most common oral diseases (caries, periodontitis and candidiasis), as well as in oral cancer prevention. PMID:22376030

  18. Characterisation of polyphenols in Terminalia arjuna bark extract

    Directory of Open Access Journals (Sweden)

    Anumita Saha

    2012-01-01

    Full Text Available The bark of Terminalia arjuna is known for its heart-health benefits in ayurvedic literature. This has been further supported by in vivo studies on animal and human volunteers. But there is no detailed study on identification of the active ingredients such as polyphenols. Polyphenols possesses antioxidant properties and are well-known health actives, it is important to characterise polyphenols in Terminalia arjuna. Aqueous extract of Terminalia arjuna bark was analysed for its composition and molecular weight distribution by dialysis. Compositional analysis revealed that it has 44% polyphenols and dialysis study showed that 70% of the polyphenols have molecular weight greater than 3.5 kDa. High performance liquid chromatography and liquid chromatography-mass spectrometry analysis of Terminalia arjuna, confirmed that it contains flavon-3-ols such as (+-catechin, (+-gallocatechin and (−-epigallocatechin. Phenolic acids such as gallic acid, ellagic acid and its derivatives were also found in Terminalia arjuna extract. Ellagic acid derivatives were isolated and their spectral studies indicated that isolated compounds were 3-O-methyl-ellagic acid 4- O-β-D-xylopyranoside, ellagic acid and 3-O-methyl ellagic acid 3-O-rhamnoside. Hydrolysis and thiolysis studies of high molecular weight polyphenols indicated that they are proanthocyanidins. Given these results, it may be possible to attribute the heart-health effects of Terminalia arjuna to these polyphenols which may be responsible for the endothelial benefit functions like tea.

  19. Chemical composition and sensory evaluation of fermented tea with medicinal mushrooms.

    Science.gov (United States)

    Bai, Wei-Fang; Guo, Xin-Yue; Ma, Li-Qing; Guo, Li-Qiong; Lin, Jun-Fang

    2013-03-01

    In commercial tea production, plenty of tea leaf waste is generated, which may not only exert pollution risk to environment, but also a huge waste of bioactive ingredients in tea. In this study, the 4th to 7th leaves of tea bush were collected and used as substrate for mycelial culture of two renown medicinal mushrooms Grifola frondosa and Tianzhi (new variants of Ganoderma lucidum) to obtain a new type of solid-state fermented tea. Result showed that the polysaccharides of Grifola frondosa and Tianzhi fermented tea were 1.52 and 4.14 %, tea polyphenols were 1.51 and 1.85 %, the free amino acids were 1.52 and 0.94 %, caffeine were 1.16 and 1.70 %, polyphenols/amid acids ratio were 1.0 and 1.98, water extractions were 35.53 and 32.86 %, protein contents were 17.63 and 6.13 mg/g, respectively. The volatile components were mainly composed of alcohols, esters, aldehydes and ketones. The contents of major flavor compositions of fermented tea had changed and their relation tended to be harmonious, and the variety of amino acids significantly increased. Therefore, the sensory flavor and therapeutic qualities of fermented tea were significantly improved. PMID:24426081

  20. A Possible Effect of Concentrated Oolong Tea Causing Transient Ischemic Attack-Like Symptoms

    OpenAIRE

    Layher, John W.; Poling, Jon S.; Ishihara, Mayumi; Azadi, Parastoo; Alvarez-Manilla, Gerardo; Puett, David

    2013-01-01

    Aims Tea (green, oolong, and black) is the second most widely consumed beverage worldwide, second only to water. Aside from a few reported adverse effects, tea, particularly green tea, appears to be beneficial for human health. In the case described herein, a male experienced several transient ischemic attack-like symptoms immediately following the consumption of a cup of high quality oolong tea. A thorough medical evaluation uncovered no evidence of such an attack and leads to the suggestion...

  1. A Possible Effect of Concentrated Oolong Tea Causing Transient Ischemic Attack-Like Symptoms

    OpenAIRE

    Layher Jr., John W.; Poling, Jon S.; Ishihara, Mayumi; Azadi, Parastoo; Alvarez-Manilla, Gerardo; Puett, David

    2013-01-01

    Aims: Tea (green, oolong, and black) is the second most widely consumed beverage worldwide, second only to water. Aside from a few reported adverse effects, tea, particularly green tea, appears to be beneficial for human health. In the case described herein, a male experienced several transient ischemic attack-like symptoms immediately following the consumption of a cup of high quality oolong tea. A thorough medical evaluation uncovered no evidence of such an attack and leads to the suggestio...

  2. Drinking Tea May Lower Bad Cholesterol

    Institute of Scientific and Technical Information of China (English)

    Christopher; Doering; 郑凤听

    2003-01-01

    喝茶,有益于健康。本文所说的是“红茶”(black tea),而非“绿茶”(green tea)。在美国喝茶者简直与时俱增:…total sales of tea in 2002 were$5.03 billion,up from$1.84 billion in 1990!本文用深入浅出的语言对喝茶的益处作了说明,同时,其文字表达也不乏可以品味处,如下句中的动词saw:Those who bad regular tea saw their LDL levels drop about 11 percent comparedwith the caffeine placebo.

  3. 有氧运动与补充茶多酚对高脂膳食大鼠糖脂代谢及炎症因子TNF-α和IL-6的影响%Effect of Aerobic Exercise and Supplement of Tea Polyphenols on Glucose and Lipid Metabolism and Lipid Inflammatory Cyto

    Institute of Scientific and Technical Information of China (English)

    马涛; 王法涛; 单小忠

    2011-01-01

    目的:研究有氧运动和补充茶多酚对高脂膳食大鼠糖脂代谢及炎症因子TNF-α和IL-6的影响,并探讨炎症因子TNF-α和IL-6在营养性肥胖所致的糖脂代谢异常及胰岛素抵抗中的作用。方法:3周龄SD雄性大鼠50只,随机分为普通膳食对照组(C组)、高脂膳食对照组(H组)、有氧运动组(HE组)、茶多酚组(HT组)和综合干预组(HET组)。C组以普通饲料饲养,其余各组均以高脂饲料饲养;HE组和HET组大鼠进行为期8周的无负重游泳训练;HT组和HET组大鼠每周6 D以1 g/kg体重剂量的茶多酚灌胃。8周后测试大鼠糖脂%Objective: Study of the effect of aerobic exercise and supplement of tea polyphenols on glucose and lipid metabolism and lipid inflammatory cytokines TNF-α and IL-6 in high-fat diet rats,and analyze the role of inflammatory cytokines TNF-α and IL-6 in the

  4. GC/MS Analysis of Volatile Components in Black Tea Extract and its Application in Cigarette%红茶提取物挥发性成分GC/MS分析及在卷烟中的应用

    Institute of Scientific and Technical Information of China (English)

    谢清桃; 王坤波; 董颖; 姚瑞; 谢春桥; 李建军; 董德华

    2012-01-01

    为开发和丰富烟用天然香精香料,采用同时蒸馏萃取法提取正山小种红茶提取物挥发性成分,用GC/MS法对挥发性成分进行了分离鉴定,共鉴定出35种挥发性成分,主要有苯乙醛、香叶醇、芳樟醇及氧化物、苯乙醇、苯甲酸苄酯、棕榈酸乙酯、植醇、亚油酸乙酯、亚麻酸乙酯等。将该提取物用于卷烟加香,能够明显丰富烟香,减小刺激性和改善卷烟余味。%In order to develop a new natural cigarette flavor, the volatile oil from the Lapsang Souchong black tea ex-tract was extracted by SDE and analyzed by GC/MS. The results showed that 35 volatile constituents were identified in the oil and the major components were benzeneacetaldehyde, (E) - geraniol, linalool, linalool oxide, phenylethyl alcohol, benzyl benzoate, hexadecanoic acid ethyl ester, phytol, linoleic acid ethyl ester and linolenic acid ethyl ester. The black tea extract was added into cigarettes as tobacco flavoring and evaluated by panelists, and the results indicated that the ex-tract could enrich the tobacco flavor, reduce the irritancy of smoke, and improve taste obviously.

  5. 茶多酚对一次性力竭运动大鼠氧化应激和炎症反应的影响%The Effects of Tea Polyphenols Supplemnet on Oxidative Stress and Inflammatory Response in Rats after Exhaustive Exercise

    Institute of Scientific and Technical Information of China (English)

    吴秀琴; 杨威; 尹玉娇; 何少栖; 刘丽霞

    2016-01-01

    Objectives :To study the effects of tea polyphenols supplemnet on oxidative stress and inflammatory response in rats after exhaustive exercise ,find out effective interventions for re-ducing the sports injuries .Methods :Twenty-four male SD rats were randomly divided into three groups according to weight :control group(C) ,exhaustive exercise group (E) ,exhaustive exercise with Tea polyphenols (F ,dose :300mg/kg/d) .4 weeks later ,the rats performed once exhaustive exercise ,and the serum creatine kinase (CK ) ,lactate dehydrogenase (LDH ) ,ma-londialdehyde(MDA ) ,the level of inflammatory cytokine :interleukin (IL-1β ,IL-10 ) and the tumor necrosis factor alpha (TNF -a) were examined .Results :Compared with C group ,the serum CK and LDH activity and MDA content of E group rats were significantly increased af-ter exhaustive exercise (P< 0 .05);also ,the levels of pro-inflammatory cytokine TNF-a and anti-inflammatory cytokine IL-10 were significantly increased (P< 0 .01 and P<0 .01) .Com-pared with E group ,the serum CK and LDH activity and MDA content of F group rats signifi-cantly decreased (P<0 .05);the levels of pro-inflammatory Cytokine TNF-a and IL-1β were all significantly decreased (P< 0 .05) ,whereas the ratio of IL-10/TNF-α was significantly improved( P< 0 .05 ) .Correlation analysis showed that it appeared positive correlation MDA with CK and TNF-a (P< 0 .05) ,but with ratio of IL -10 /TNF-a ,they appeared very sig-nificantly negative correlation (P<0 .01) .Conclusion :The supplement of tea polyphenols can improve the body's antioxidant status ,reduce inflammation response and play a protective role to skeletal muscle in rat performing exhaustive exercise .%目的 :探讨茶多酚对力竭运动大鼠氧化应激和炎症因子的影响 ,为减少运动损伤寻找有效的干预手段.方法 :24只雄性SD大鼠按体重随机分为对照组(C )、力竭组(E )和茶多酚力竭组(F ,300 mg/kg/d茶多酚灌胃) ,4周后 ,

  6. Determination of isotopic composition of strontium in black tea samples from India by Multicollector Inductively Coupled Plasma Mass Spectrometry (MC-ICPMS)

    International Nuclear Information System (INIS)

    Identification of geographical origins of food materials is of great importance due to rampant mislabeling that is usually done for getting a higher market price for the agricultural produce. Several works have been undertaken to identify the provenance of tea (Camellia Sinensis). The geographical identification is based on trace elemental profiling of the samples as well as stable isotope ratio signatures. Significant amount of work is also reported for a wide range of food samples such as Coffee, honey, rice etc.

  7. Modulatory Effects of Polyphenols on Apoptosis Induction: Relevance for Cancer Prevention

    OpenAIRE

    Claudio Giovannini; Roberta Masella; Carmela Filesi; Rosaria Varì; Beatrice Scazzocchio; Carmela Santangelo; Massimo D’Archivio

    2008-01-01

    Polyphenols, occurring in fruit and vegetables, wine, tea, extra virgin olive oil, chocolate and other cocoa products, have been demonstrated to have clear antioxidant properties in vitro, and many of their biological actions have been attributed to their intrinsic reducing capabilities. However, it has become clear that, in complex biological systems, polyphenols exhibit several additional properties which are yet poorly understood. Apoptosis is a genetically controlled and evolutionarily co...

  8. Lipid oxidation degree and antioxidant activity of several polyphenolic extracts in bovine meat during storage

    OpenAIRE

    Ilir Lloha; Vlash Mara

    2013-01-01

    Extracts of vegetable origin are used widely nowdays in the food industry in the role of antioxidants, especially in the meat processing industry and in the industry of its byproducts. Subject of this study have been bovine meat samples, which have been subjected to polyphenolic extracts, such as those from: tea, rosemary and oregano conserved in a timeframe of 1, 4, 7 and 10 days. TBA (thiobarbituric acid) assay shows that polyphenolic extracts tend to increase oxidative endurance of meat sa...

  9. Lipid oxidation degree and antioxidant activity of several polyphenolic extracts in porcine meat during storage

    OpenAIRE

    ILIR LLOHA; VLASH MARA

    2014-01-01

    Extracts of vegetable origin are used widely nowdays in the food industry in the role of antioxidants, especially in the meat processing industry and in the industry of its byproducts. Subject of this study have been porcine meat samples, which have been subjected to polyphenolic extracts, such as those from: tea, rosemary and oregano conserved in a timeframe of 1, 4, 7 and 10 days. TBA (thiobarbituric acid) assay show that polyphenolic extracts tend to increase oxidative endurance of meat sa...

  10. Dietary polyphenols and colorectal cancer risk: The Fukuoka colorectal cancer study

    OpenAIRE

    Zhen-Jie Wang; Keizo Ohnaka; Makiko Morita; Kengo Toyomura; Suminori Kono; Takashi Ueki; Masao Tanaka; Yoshihiro Kakeji; Yoshihiko Maehara; Takeshi Okamura; Koji Ikejiri; Kitaroh Futami; Takafumi Maekawa; Yohichi Yasunami; Kenji Takenaka

    2013-01-01

    AIM: To investigate the associations between dietary intake of polyphenols and colorectal cancer. METHODS: The study subjects were derived from the Fukuoka colorectal cancer study, a community-based case-control study. The study subjects were 816 cases of colorectal cancer and 815 community-based controls. The consumption of 148 food items was assessed by a computer-assisted interview. We used the consumption of 97 food items to estimate dietary intakes of total, tea and coffee polyphenols. T...

  11. Using UV-Vis spectroscopy for simultaneous geographical and varietal classification of tea infusions simulating a home-made tea cup.

    Science.gov (United States)

    Diniz, Paulo Henrique Gonçalves Dias; Barbosa, Mayara Ferreira; de Melo Milanez, Karla Danielle Tavares; Pistonesi, Marcelo Fabián; de Araújo, Mário César Ugulino

    2016-02-01

    In this work we proposed a method to verify the differentiating characteristics of simple tea infusions prepared in boiling water alone (simulating a home-made tea cup), which represents the final product as ingested by the consumers. For this purpose we used UV-Vis spectroscopy and variable selection through the Successive Projections Algorithm associated with Linear Discriminant Analysis (SPA-LDA) for simultaneous classification of the teas according to their variety and geographic origin. For comparison, KNN, CART, SIMCA, PLS-DA and PCA-LDA were also used. SPA-LDA and PCA-LDA provided significantly better results for tea classification of the five studied classes (Argentinean green tea; Brazilian green tea; Argentinean black tea; Brazilian black tea; and Sri Lankan black tea). The proposed methodology provides a simpler, faster and more affordable classification of simple tea infusions, and can be used as an alternative approach to traditional tea quality evaluation as made by skilful tasters, which is evidently partial and cannot assess geographic origins. PMID:26304362

  12. Effect of tea on iron absorption from the typical Tunisian meal 'couscous' fed to healthy rats.

    Science.gov (United States)

    Hamdaoui, M; Hedhili, A; Doghri, T; Tritar, B

    1994-01-01

    Black and green tea decoctions are popular beverages in Tunisia, especially after eating. Our study was performed to examine the effect of graded amounts of black and green tea decoction prepared under realistic Tunisian conditions on nonheme iron absorption from a typical Tunisian meal, 'couscous', by extrinsic radioiron labeling in rats. Concentrations of 300, 200 and 100 micrograms/ml of black tea decreased dramatically nonheme iron bioavailability from couscous, but 50 micrograms/ml did not influence iron absorption. The inhibition of nonheme iron from couscous varied from 36 to 61% with black tea and 30.5% with green tea. Taken together, our findings show that the tea decoction in Tunisia has a great inhibitory power and may constitute an important factor for the development of iron deficiency anemia throughout Tunisia. PMID:7832583

  13. 坦洋工夫红茶科技创新与产业策略分析%Science Innovation and Industry Strategy Analysis on Panyong Congou Black Tea

    Institute of Scientific and Technical Information of China (English)

    杨如兴

    2013-01-01

    According to the good inheritance and development situation of panyong congou industry,the eight major science and technology innovation of panyong congou in the production were analyzed.① Tea resources and innovation and application of the high-aroma pattern varieties; ②Integration and innovation of the low-carbon,efficient and ecological cultivation technology; ③Innovation of processing technology on the flower-fruit aroma black tea; ④The development of standard and innovation of processing machinery; ⑤Originality and innovation of packaging and culture; ⑥ Innovation of selling idea and network platform; ⑦Skill innovation of drinking and brewing process; ⑧Model innovation of brand construction.Then the creativeness ideas of trade name,packaging,culture,journey on panyong congou were investigate.Finally the industry development countermeasure were put forwarded,including stabilizing area of tea garden,taking advantage of variety,development characteristic product,supporting system authentication,implementation of GAP management,establishing tracing back system,guiding transformation of production mode,developing low-carbon,high profit and product differentiation road,attempting tea-manor management.The purpose was to promote transformation and upgrading of panyong congou black tea industry.%针对近年坦洋工夫红茶产业的良好传承和发展现状,分析了当前坦洋工夫生产8项科技创新:①茶树资源与高香型品种创新应用;②低碳高效生态栽培技术集成创新;③花果香红茶加工技术创新;④红茶标准研制与加工机械创新;⑤包装及其文化创意创新;⑥销售理念与网络平台创新;⑦茶叶冲泡技艺创新;⑧品牌建设模式创新.提出坦洋工夫“品名、包装、文化、茶旅”等创意思路和“稳定茶园面积、发挥品种优势、研发特色产品,支持体系认证、实现GAP管理、建立追溯体系,引导生产方式转变、走低碳高效与产品差

  14. Critical factors determining fluoride concentration in tea leaves produced from Anhui province, China.

    Science.gov (United States)

    Cai, Huimei; Zhu, Xiaohui; Peng, Chuanyi; Xu, Wei; Li, Daxiang; Wang, Yijun; Fang, Shihui; Li, Yeyun; Hu, Shaode; Wan, Xiaochun

    2016-09-01

    This study investigated the fluoride present in tea plants (Camellia sinensis (L.) O. Kuntze) and its relationship to soils, varieties, seasons and tea leaf maturity. The study also explored how different manufacturing processes affect the leaching of fluoride into tea beverages. The fluoride concentration in the tea leaves was significantly correlate to the concentration of water-soluble fluoride in the soil. Different tea varieties accumulated different levels of fluoride, with varieties, Anji baicha having the highest and Nongkang zao having the lowest fluoride concentration. In eight different varieties of tea plant harvested over three tea seasons, fluoride concentration were highest in the summer and lowest in the spring in china. The fluoride concentration in tea leaves was directly related to the maturity of the tea leaves at harvest. Importantly, the tea manufacturing process did not introduced fluoride contamination. The leaching of fluoride was 6.8% and 14.1% higher in black and white tea, respectively, than in fresh tea leaves. The manufacturing step most affecting the leaching of fluoride into tea beverage was withering used in white, black and oolong tea rather than rolling or fermentation. The exposure and associated health risks for fluoride concentration in infusions of 115 commercially available teas from Chinese tea markets was determined. The fluoride concentration ranged from 5.0 to 306.0mgkg(-1), with an average of 81.7mgkg(-1). The hazard quotient (HQ) of these teas indicated that there was no risk of fluorosis from drinking tea, based on statistical analysis by Monte Carlo simulation. PMID:27162130

  15. Tea Tree Oil

    Science.gov (United States)

    96262 ... https://nccih.nih.gov/health/tea/treeoil.htm ... us ... 96262 ... https://nccih.nih.gov/health/tea/treeoil.htm ... Herbal Medicine ... Herbal Medicine/Specifics ... us ... 96262 ... https:// ...

  16. Interactions between tea catechins and casein micelles and their impact on renneting functionality.

    Science.gov (United States)

    Haratifar, Sanaz; Corredig, Milena

    2014-01-15

    Many studies have shown that tea catechins bind to milk proteins. This research focused on the association of tea polyphenols with casein micelles, and the consequences of the interactions on the renneting behaviour of skim milk. It was hypothesized that epigallocatechin-gallate (EGCG), the main catechin present in green tea, forms complexes with the casein micelles and that the association modifies the processing functionality of casein micelles. The binding of EGCG to casein micelles was quantified using HPLC. The formation of catechin-casein micelles complexes affected the rennet induced gelation of milk, and the effect was concentration dependent. Both the primary as well as the secondary stage of gelation were affected. These experiments clearly identify the need for a better understanding of the effect of tea polyphenols on the processing functionality of casein micelles, before milk products can be used as an appropriate platform for delivery of bioactive compounds. PMID:24054208

  17. 红茶风味肉脯的加工方法%Processing method of dried meat slice with tea fragrance

    Institute of Scientific and Technical Information of China (English)

    杨锋; 张晓军; 徐丽娜

    2015-01-01

    通过添加天然红茶粉和茶多酚,改进传统肉脯配方,突破传统肉脯加工工艺,采用冷风干燥、天然木碳碳烤、电烤等现代生产加工技术,改善肉脯组织柔软度,提升口感和光泽度,同时赋予肉脯天然独特的碳烤肉风味和红茶风味。%The formula of traditional dried meat slice was improved. And processing technology of traditional dried meat slice was broken through by addition of black tea powder and tea polyphenols. Cold air drying,roasting by natural wood charcoal,electric roasting and other modern producing and processing technology were taken to improve softness of the dried meat's texture and promote the taste and lustrous⁃ness. Meanwhile the dried meat slice was given with unique natural charcoal roasting flavor and tea fla⁃vor.

  18. Drifting Fragrant Tea

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    ORIGINATING in China, tea drinking as well as tea culture has a long standing history of three to four thousand years. The formal tea ceremony had been in gradual decline since the Ming (1368-1644) and Qing (1644-1911) dynasties but gradually began to re-develop in the 1980s owing to the demands of people from all walks of life. The prosperity of the local tea culture is witnessed by the

  19. 茶叶加工中品质关键组分的变化与调控机制%The Change and Regulation Mechanism of Key Components during Tea Processing

    Institute of Scientific and Technical Information of China (English)

    莫婷; 张婉璐; 李平

    2011-01-01

    Tea has originated from China, and it is one of the three world's popular beverage. A variety of compounds, such as polyphenols, proteins, amino acids, alkaloids, aromatic compounds, carbohydrates, lipids, vitamins, minerals, chlorophyll, enzymes, were rich in tea. After being non-fermented, slightly fermented, semi-fermented, fermented and secondary fermented which include withering, fixing, rolling, fermentation and drying treatment, catechins were oxidized and polymerized to theaflavins, thearuhigins and theabrownin by polyphenol oxidase and peroxidase because of the effect of temperature, pressure, enzymes and microorganisms. In addition, aroma precursors, β-D-glycosides were hydrolyzed into the correspongding alcohols and other aroma substances under the action of β -glucosidase andβ -primeverosidase. Proteins were hydrolyze into amino acids and further formed different chiral enantiomers. These would endow the unique color of tea, aroma, taste and appearance. Tea has many functions, such as antibacterial, antiviral, antioxidant, anti-aging, anti-cancer, immune enhancement, prevented of cardiovascular disease and so on. Now tea was classified into green tea, white tea, yellow tea, oolong tea, black tea and puerh tea. This paper explained the relevance and problems between the change regulation and technical control in the process from the key components of tea such as color, aroma and flavor. Moreover, the formation mechanism to provide a theoretical basis for improving the quality of tea was also discussed.%茶树起源于中国,茶叶是世界三大饮品之一.茶叶中富含茶多酚、蛋白质及氨基酸、生物碱、芳香族化合物、糖类、类脂化合物、维生素、无机物、叶绿素、酶等多种化合物.它们经过非发酵、微发酵、半发酵、全发酵及后发酵工艺,萎凋、杀青、揉捻、发酵、干燥等处理后,由于温度、压力、酶及微生物的作用,儿茶素在多酚氧化酶、过氧化物酶等的

  20. HPLC-MS/MS Analysis of Poly phenols in Different Fermented Type Tea Products Prepared from Camellia sinensis'Baiye Dancong'%白叶单枞不同发酵茶中多酚类成分的HPLC-MS/MS分析

    Institute of Scientific and Technical Information of China (English)

    苗爱清; 程悦; 梁祈; 赵超艺; 凌彩金; 庞式; 王冬梅

    2011-01-01

    Chemical compositions of broken black tea, oolong tea and dark tea prepared from Camellia sinensis ‘Baiye Dancong’ were analyzed by HPLC-MS/MS method.Comparing the UV absorption spectra and mass spectra of the peaks in the obtained HPLC chromatograms with those of the authentic standards and related literatures, 32, 30 and 14 compounds were identified from broken black tea, oolong tea and dark tea respectively.These compounds included catechins, alkaloids.Some non-catechin polyphenol compounds such as flavonol glycosides with kaempferol, quercetin and myricitin as aglycones, and catechin polymers such as theasinensins were also identified.%采用HPLC-MS/MS对以白叶单枞茶为原料生产的红碎茶、乌龙茶、黑茶进行分析,通过对紫外吸收光谱以及质谱信息进行分析,并参考相关文献,推定出白叶单枞红茶中32个化合物,乌龙茶中30个化合物,黑茶中14个化合物.这些化合物中包括常见的儿茶素类化合物、生物碱.此外还检测到多种以山奈酚、槲皮素、杨梅素为苷元的黄酮醇苷等非儿茶素类茶多酚和茶双没食子儿茶素等儿茶素聚合物.

  1. Antioxidative potential, nutritional value and sensory profiles of confectionery fortified with green and yellow tea leaves (Camellia sinensis).

    Science.gov (United States)

    Gramza-Michałowska, Anna; Kobus-Cisowska, Joanna; Kmiecik, Dominik; Korczak, Józef; Helak, Barbara; Dziedzic, Krzysztof; Górecka, Danuta

    2016-11-15

    This paper presents a study on development of functional food products containing green and yellow tea leaves. The results indicated that green and yellow tea are significant tools in the creation of the nutritional value, antioxidative potential and stability of the lipid fraction of cookies. Tea-fortified cookies showed considerably higher contents of dietary fiber, especially hemicellulose and insoluble fractions, and were characterized by significantly higher antioxidant potential associated with their phenolics content. Results of ABTS, DPPH, ORACFL and PCL assay showed significantly higher antioxidant potential of tea cookies, highest for yellow tea. The antioxidative potential of applied teas was significant in terms of the inhibition of hydroperoxide content, while formation of secondary lipid oxidation products was less spectacular. It is concluded that tea leaves could be widely used as a source of polyphenols with high antioxidative potential, as well as fiber; thus introducing numerous health benefits for the consumer. PMID:27283654

  2. Randomized controlled trial for an effect of green tea consumption on insulin resistance and inflammation markers.

    Science.gov (United States)

    Fukino, Yoko; Shimbo, Mari; Aoki, Nobuo; Okubo, Tutomu; Iso, Hiroyasu

    2005-10-01

    To study the effects of the intake of green tea and polyphenols, which are a component of green tea, on insulin resistance and systemic inflammation, a randomized controlled trial was conducted on 66 patients aged 32-73 y (53 males and 13 females) with borderline diabetes or diabetes. Subjects in the intervention group were asked to take a packet of green tea extracts/powder containing 544 mg polyphenols (456 mg catechins) daily, which was a dose that could be taken without difficulty, and were asked to divide the green tea extracts/powder in a packet into 3 or 4 fractions dissolved in hot water everyday and to take a fraction after every meal or snack for 2 mo, in addition to daily food intake. The subjects in the control group were simply followed. To calculate the level of green tea polyphenol intake that the subject usually drank at home, the subject was asked to taste 3 teas of different strengths (1, 2 and 3%) and the tea that was closest to the one that the subject drank at home, was selected by each subject. After 2 mo, the mean daily polyphenol intake in the intervention group was 747 mg, which was significantly higher than that of 469 mg in the control group. In the intervention group, the body weight, BMI, systolic and diastolic blood pressures, blood glucose level, Hb A1c level, insulin level and HOMA index after taking the supplementation for 2 mo, were lower than the respective value before intervention: however, these parameters in the intervention group at 2 mo did not significantly differ from those in the control group. Within the intervention group, changes in insulin level tended to be associated with changes in polyphenol intake. In addition, changes in BMI were associated with changes in blood glucose level and insulin level. In conclusion, the daily supplementary intake of 500 mg green tea polyphenols did not have clear effects on blood glucose level, Hb A1c level, insulin resistance or inflammation markers. The positive correlation between

  3. Isolation of Cronobacter sakazakii from different herbal teas

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    Stojanović Marija M.

    2011-01-01

    Full Text Available Bacground/Aim. Cronobacter sakazakii (C. sakazakii is an emerging food-borne pathogen that has increasingly raised interest among the whole public community and food industry, especially in the production of powder infant formula. It has been isolated from water, sediment and soil. The question is whether this pathogen can be present in herbal teas. Herbal teas are widely used for great number of health problems, as an additional or sometimes only “medicine” given. The aim of this study was to investigate the presence of C. sakazakii in herbal teas which are traditionally used for all restricted populations, including newborns and immunocompromised infant and adults. Methods. In this study 150 samples of dried herbal teas were tested: Children (Baby tea (11, Althaea officinalis (7, Sennae folium (4, Mentha piperita (8, Hypericum perforatum (3, Thymus serpyllum (5, Matricaria recutita (6, Fruit tea (18, Black, Green and Rooibos tea (11, Salvia officinalis (9, Arctostaphylos uva ursi (5, Urtica dioica (3, Achillea millefolium (2, Melissa officinalis (4, Cynosbati fructus (3, Flower Herbal tea (3 and 17 different mixtures of tea (48 samples. The presence of C. sakazakii was also investigated in previously positive samples of prepared teas (48 samples after 2 h, 12 h and 24 h. C. sakazakii was isolated by the use of the official method ISO TS 22964 : 2006 and confirmed with the biochemical test API 20E (Biomerieux-France. Results. The obtained results showed that C. sakazakii was isolated from 48 (32% samples dried herbal teas. C. sakazakii was not isolated only from 2 (4% of the 48 tested samples of prepared tea and in 46 (96% of the samples C. sakazakii remained viable after 2 h, 12 h and 24 h. Conclusion. Herbal teas should be carefully used, especially for infants and immunocompromited people with severe chronic diseases because of the possibility of infection by C. sakazakii. Better control and improve testing as well as new facts about

  4. (−)-Epigallocatechin Gallate, A Major Constituent of Green Tea, Poisons Human Type II Topoisomerases†

    OpenAIRE

    Bandele, Omari J.; Osheroff, Neil

    2008-01-01

    (−)-Epigallocatechin gallate (EGCG) is the most abundant and biologically active polyphenol in green tea, and many of the therapeutic benefits of the beverage have been attributed to this compound. High concentrations of EGCG are cytotoxic and trigger genotoxic events in mammalian cells. Although this catechin affects a number of cellular systems, the genotoxic effects of several bioflavonoid-based dietary polyphenols are believed to be mediated, at least in part, by their actions on topoisom...

  5. White Tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)-adipocytes

    OpenAIRE

    Wenck Horst; Gallinat Stefan; Schepky Andreas; Grönniger Elke; Siegner Ralf; Holtzmann Ursula; Knott Anja; Söhle Jörn; Stäb Franz; Winnefeld Marc

    2009-01-01

    Abstract Background The dramatic increase in obesity-related diseases emphasizes the need to elucidate the cellular and molecular mechanisms underlying fat metabolism. To investigate how natural substances influence lipolysis and adipogenesis, we determined the effects of White Tea extract on cultured human subcutaneous preadipocytes and adipocytes. Methods For our in vitro studies we used a White Tea extract solution that contained polyphenols and methylxanthines. Utilizing cultured human pr...

  6. Binding of Natural and Synthetic Polyphenols to Human Dihydrofolate Reductase

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    José Neptuno Rodríguez-López

    2009-12-01

    Full Text Available Dihydrofolate reductase (DHFR is the subject of intensive investigation since it appears to be the primary target enzyme for antifolate drugs. Fluorescence quenching experiments show that the ester bond-containing tea polyphenols (--epigallocatechin gallate (EGCG and (--epicatechin gallate (ECG are potent inhibitors of DHFR with dissociation constants (KD of 0.9 and 1.8 μM, respectively, while polyphenols lacking the ester bound gallate moiety [e.g., (--epigallocatechin (EGC and (--epicatechin (EC] did not bind to this enzyme. To avoid stability and bioavailability problems associated with tea catechins we synthesized a methylated derivative of ECG (3-O-(3,4,5-trimethoxybenzoyl-(--epicatechin; TMECG, which effectively binds to DHFR (KD = 2.1 μM. In alkaline solution, TMECG generates a stable quinone methide product that strongly binds to the enzyme with a KD of 8.2 nM. Quercetin glucuronides also bind to DHFR but its effective binding was highly dependent of the sugar residue, with quercetin-3-xyloside being the stronger inhibitor of the enzyme with a KD of 0.6 μM. The finding that natural polyphenols are good inhibitors of human DHFR could explain the epidemiological data on their prophylactic effects for certain forms of cancer and open a possibility for the use of natural and synthetic polyphenols in cancer chemotherapy.

  7. Psychiatric Disorders and Polyphenols: Can They Be Helpful in Therapy?

    Science.gov (United States)

    Trebatická, Jana; Ďuračková, Zdeňka

    2015-01-01

    The prevalence of psychiatric disorders permanently increases. Polyphenolic compounds can be involved in modulation of mental health including brain plasticity, behaviour, mood, depression, and cognition. In addition to their antioxidant ability other biomodulating properties have been observed. In the pathogenesis of depression disturbance in neurotransmitters, increased inflammatory processes, defects in neurogenesis and synaptic plasticity, mitochondrial dysfunction, and redox imbalance are observed. Ginkgo biloba, green tea, and Quercus robur extracts and curcumin can affect neuronal system in depressive patients. ADHD patients treated with antipsychotic drugs, especially stimulants, report significant adverse effects; therefore, an alternative treatment is searched for. An extract from Ginkgo biloba and from Pinus pinaster bark, Pycnogenol, could become promising complementary supplements in ADHD treatment. Schizophrenia is a devastating mental disorder, with oxidative stress involved in its pathophysiology. The direct interference of polyphenols with schizophrenia pathophysiology has not been reported yet. However, increased oxidative stress caused by haloperidol was inhibited ex vivo by different polyphenols. Curcumin, extract from green tea and from Ginkgo biloba, may have benefits on serious side effects associated with administration of neuroleptics to patients suffering from schizophrenia. Polyphenols in the diet have the potential to become medicaments in the field of mental health after a thorough study of their mechanism of action. PMID:26180581

  8. Psychiatric Disorders and Polyphenols: Can They Be Helpful in Therapy?

    Directory of Open Access Journals (Sweden)

    Jana Trebatická

    2015-01-01

    Full Text Available The prevalence of psychiatric disorders permanently increases. Polyphenolic compounds can be involved in modulation of mental health including brain plasticity, behaviour, mood, depression, and cognition. In addition to their antioxidant ability other biomodulating properties have been observed. In the pathogenesis of depression disturbance in neurotransmitters, increased inflammatory processes, defects in neurogenesis and synaptic plasticity, mitochondrial dysfunction, and redox imbalance are observed. Ginkgo biloba, green tea, and Quercus robur extracts and curcumin can affect neuronal system in depressive patients. ADHD patients treated with antipsychotic drugs, especially stimulants, report significant adverse effects; therefore, an alternative treatment is searched for. An extract from Ginkgo biloba and from Pinus pinaster bark, Pycnogenol, could become promising complementary supplements in ADHD treatment. Schizophrenia is a devastating mental disorder, with oxidative stress involved in its pathophysiology. The direct interference of polyphenols with schizophrenia pathophysiology has not been reported yet. However, increased oxidative stress caused by haloperidol was inhibited ex vivo by different polyphenols. Curcumin, extract from green tea and from Ginkgo biloba, may have benefits on serious side effects associated with administration of neuroleptics to patients suffering from schizophrenia. Polyphenols in the diet have the potential to become medicaments in the field of mental health after a thorough study of their mechanism of action.

  9. Green tea extract decreases oxidative stress and improves insulin sensitivity in an animal model of insulin resistance, the fructose- fed rat

    Science.gov (United States)

    Metabolic syndrome is characterized by insulin resistance, dyslipidemia, and increased oxidative stress. Tea polyphenols, as both insulin potentiating factors and antioxidants, might act in preventing the metabolic syndrome. We aimed to determine the effects of green tea extract consumption on oxida...

  10. Green tea Improves Carbohydrate and Lipid Metabolism and Regulates Cardiac mRNA Expression Related to Insulin, Lipid and Inflammatory Signaling Pathways

    Science.gov (United States)

    Green tea has multiple benefits to human health. Epidemiologic studies demonstrate that the consumption of green tea polyphenols (GTP) reduces the risk of coronary artery disease and cardiac events. To explore the underlying mechanisms of action at a molecular level, we examined the effects of GTP o...

  11. Comparison of the mineral composition of leaves and infusions of traditional and herbal teas

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    Isaac T. Rampedi

    2012-01-01

    Full Text Available Most research on teas has focused on organic composition and less attention has been given to the mineral composition. The aim of this study was to examine and compare the mineral compositions (Na, Mg, K, Ca, P, S, Fe, Mn, Zn, Cu and Al of eight commonly consumed teas. The teas included three traditional black or green teas (from Africa, China and Sri Lanka and five herbal teas – two from South America (maté and coca and three from South Africa (rooibos, honeybush and Athrixia phylicoides. Analyses were conducted on five samples of dry tea leaves of each of the teas and their infusions (steeping time: 6 min using identical techniques in inductively coupled plasma optical emission spectrometry (ICP-OES. It was found that each tea has a unique mineral profile. Dry tea leaves and their respective infusions also exhibited different mineral profiles. The tea infusions that contained relatively higher concentrations of beneficial minerals were maté, coca and Athrixia. High levels of aluminium were found in the traditional black and green teas whilst rooibos was high in sodium. Although teas are not rich sources of nutrients, the consumption of maté could contribute significantly to dietary manganese requirements.

  12. Systematic Analysis of the Multiple Bioactivities of Green Tea through a Network Pharmacology Approach

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    Shoude Zhang

    2014-01-01

    Full Text Available During the past decades, a number of studies have demonstrated multiple beneficial health effects of green tea. Polyphenolics are the most biologically active components of green tea. Many targets can be targeted or affected by polyphenolics. In this study, we excavated all of the targets of green tea polyphenolics (GTPs though literature mining and target calculation and analyzed the multiple pharmacology actions of green tea comprehensively through a network pharmacology approach. In the end, a total of 200 Homo sapiens targets were identified for fifteen GTPs. These targets were classified into six groups according to their related disease, which included cancer, diabetes, neurodegenerative disease, cardiovascular disease, muscular disease, and inflammation. Moreover, these targets mapped into 143 KEGG pathways, 26 of which were more enriched, as determined though pathway enrichment analysis and target-pathway network analysis. Among the identified pathways, 20 pathways were selected for analyzing the mechanisms of green tea in these diseases. Overall, this study systematically illustrated the mechanisms of the pleiotropic activity of green tea by analyzing the corresponding “drug-target-pathway-disease” interaction network.

  13. Selected Tea and Tea Pomace Extracts Inhibit Intestinal α-Glucosidase Activity in Vitro and Postprandial Hyperglycemia in Vivo

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    Jungbae Oh

    2015-04-01

    Full Text Available Type 2 diabetes mellitus (T2DM is a metabolic disorder characterized by postprandial hyperglycemia, which is an early defect of T2DM and thus a primary target for anti-diabetic drugs. A therapeutic approach is to inhibit intestinal α-glucosidase, the key enzyme for dietary carbohydrate digestion, resulting in delayed rate of glucose absorption. Although tea extracts have been reported to have anti-diabetic effects, the potential bioactivity of tea pomace, the main bio waste of tea beverage processing, is largely unknown. We evaluated the anti-diabetic effects of three selected tea water extracts (TWE and tea pomace extracts (TPE by determining the relative potency of extracts on rat intestinal α-glucosidase activity in vitro as well as hypoglycemic effects in vivo. Green, oolong, and black tea bags were extracted in hot water and the remaining tea pomace were dried and further extracted in 70% ethanol. The extracts were determined for intestinal rat α-glucosidases activity, radical scavenging activity, and total phenolic content. The postprandial glucose-lowering effects of TWE and TPE of green and black tea were assessed in male Sprague-Dawley (SD rats and compared to acarbose, a known pharmacological α-glucosidase inhibitor. The IC50 values of all three tea extracts against mammalian α-glucosidase were lower or similar in TPE groups than those of TWE groups. TWE and TPE of green tea exhibited the highest inhibitory effects against α-glucosidase activity with the IC50 of 2.04 ± 0.31 and 1.95 ± 0.37 mg/mL respectively. Among the specific enzymes tested, the IC50 values for TWE (0.16 ± 0.01 mg/mL and TPE (0.13 ± 0.01 mg/mL of green tea against sucrase activity were the lowest compared to those on maltase and glucoamylase activities. In the animal study, the blood glucose level at 30 min after oral intake (0.5 g/kg body wt of TPE and TWE of both green and black tea was significantly reduced compared to the control in sucrose-loaded SD

  14. Daily Fluoride Intake from Iranian Green Tea: Evaluation of Various Flavorings on Fluoride Release.

    Science.gov (United States)

    Maleki, Afshin; Daraei, Hiua; Mohammadi, Elham; Zandi, Shiva; Teymouri, Pari; Mahvi, Amir Hossien; Gharibi, Fardin

    2016-01-01

    With increased awareness of the health benefits of the compounds in green tea, especially polyphenols, its consumption is rising. The main purpose of this study is to determine the effect of different additives on the released fluoride into tea liquor and also daily fluoride intake. The concentrations of fluoride, nitrate, sulfate, and chloride were measured in 15 different flavored green teas (Refah-Lahijan). The fluoride and other anion concentrations were measured by ion chromatography method. The data were analyzed with Statistical Package for the Social Sciences version 16.0. The results showed that the minimum and maximum concentrations of fluoride in the green tea infusions were 0.162 mg/L (cinnamon-flavored green tea) and 3.29 mg/L (bagged peach-flavored green tea), respectively. The mean concentration of fluoride in the green tea leaves was 52 mg/kg, and approximately 89% of the fluoride was released from the green tea leaves into the infusions after brewing. The fluoride concentrations varied significantly among the examined green teas (P 0.05). Finally, drinking of the studied green teas cannot make a significant contribution to the daily dietary intake of F for consumers. PMID:27042093

  15. White tea (Camellia sinensis extract reduces oxidative stress and triacylglycerols in obese mice

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    Lílian Gonçalves Teixeira

    2012-12-01

    Full Text Available White tea is an unfermented tea made from young shoots of Camellia sinensis protected from sunlight to avoid polyphenol degradation. Although its levels of catechins are higher than those of green tea (derived from the same plant, there are no studies addressing the relationship between this tea and obesity associated with oxidative stress.The objective of this study was to evaluate the effect of white tea on obesity and its complications using a diet induced obesity model. Forty male C57BL/6 mice were fed a high-fat diet to induce obesity (Obese group or the same diet supplemented with 0.5% white tea extract (Obese + WTE for 8 weeks. Adipose tissue, serum lipid profile, and oxidative stress were studied. White tea supplementation was not able to reduce food intake, body weight, or visceral adiposity. Similarly, there were no changes in cholesterol rich lipoprotein profile between the groups. A reduction in blood triacylglycerols associated with increased cecal lipids was observed in the group fed the diet supplemented with white tea. White tea supplementation also reduced oxidative stress in liver and adipose tissue. In conclusion, white tea extract supplementation (0.5% does not influence body weight or adiposity in obese mice. Its benefits are restricted to the reduction in oxidative stress associated with obesity and improvement of hypertriacylglycerolemia.

  16. Daily Fluoride Intake from Iranian Green Tea: Evaluation of Various Flavorings on Fluoride Release

    Science.gov (United States)

    Maleki, Afshin; Daraei, Hiua; Mohammadi, Elham; Zandi, Shiva; Teymouri, Pari; Mahvi, Amir Hossien; Gharibi, Fardin

    2016-01-01

    With increased awareness of the health benefits of the compounds in green tea, especially polyphenols, its consumption is rising. The main purpose of this study is to determine the effect of different additives on the released fluoride into tea liquor and also daily fluoride intake. The concentrations of fluoride, nitrate, sulfate, and chloride were measured in 15 different flavored green teas (Refah-Lahijan). The fluoride and other anion concentrations were measured by ion chromatography method. The data were analyzed with Statistical Package for the Social Sciences version 16.0. The results showed that the minimum and maximum concentrations of fluoride in the green tea infusions were 0.162 mg/L (cinnamon-flavored green tea) and 3.29 mg/L (bagged peach-flavored green tea), respectively. The mean concentration of fluoride in the green tea leaves was 52 mg/kg, and approximately 89% of the fluoride was released from the green tea leaves into the infusions after brewing. The fluoride concentrations varied significantly among the examined green teas (P 0.05). Finally, drinking of the studied green teas cannot make a significant contribution to the daily dietary intake of F for consumers. PMID:27042093

  17. A comparative analysis of chemical compositions in Camellia sinensis var. puanensis Kurihara, a novel Chinese tea, by HPLC and UFLC-Q-TOF-MS/MS.

    Science.gov (United States)

    Li, Yi-Fang; Ouyang, Shu-Hua; Chang, Yi-Qun; Wang, Ting-Mei; Li, Wei-Xi; Tian, Hai-Yan; Cao, Hong; Kurihara, Hiroshi; He, Rong-Rong

    2017-02-01

    Camellia sinensis var. puanensis Kurihara (Puan tea) is a kind of ancient tea plant newly found in Jiangxipo and the surrounding areas of Puan County (Guizhou, China). People there always believe that drinking Puan tea is beneficial to the promotion of health and prevention of diseases. However, detailed information on its compositions has not been reported. Therefore, in this study, the varieties and contents of purine alkaloids and polyphenols in Puan tea were identified and determined by HPLC and UFLC-Q-TOF-MS/MS. Our results showed that theacrine, but not caffeine, was the dominated purine alkaloid detected in Puan tea. Meanwhile, Puan tea contained B-type procyanidin dimer, trimer and dimer monogallate, which were not detected in Camellia sinensis, Camellia ptilophylla and Camellia assamica var. kucha. The obtained results could support the local uses of Puan tea in health and nutrition and contribute to the research of tea variety. PMID:27596421

  18. Impedance study of tea with added taste compounds using conducting polymer and metal electrodes.

    Science.gov (United States)

    Dhiman, Mopsy; Kapur, Pawan; Ganguli, Abhijit; Singla, Madan Lal

    2012-09-01

    In this study the sensing capabilities of a combination of metals and conducting polymer sensing/working electrodes for tea liquor prepared by addition of different compounds using an impedance mode in frequency range 1 Hz-100 KHz at 0.1 V potential has been carried out. Classification of six different tea liquor samples made by dissolving various compounds (black tea liquor + raw milk from milkman), (black tea liquor + sweetened clove syrup), (black tea liquor + sweetened ginger syrup), (black tea liquor + sweetened cardamom syrup), (black tea liquor + sweet chocolate syrup) and (black tea liquor + vanilla flavoured milk without sugar) using six different working electrodes in a multi electrode setup has been studied using impedance and further its PCA has been carried out. Working electrodes of Platinum (Pt), Gold (Au), Silver (Ag), Glassy Carbon (GC) and conducting polymer electrodes of Polyaniline (PANI) and Polypyrrole (PPY) grown on an ITO surface potentiostatically have been deployed in a three electrode set up. The impedance response of these tea liquor samples using number of working electrodes shows a decrease in the real and imaginary impedance values presented on nyquist plots depending upon the nature of the electrode and amount of dissolved salts present in compounds added to tea liquor/solution. The different sensing surfaces allowed a high cross-selectivity in response to the same analyte. From Principal Component Analysis (PCA) plots it was possible to classify tea liquor in 3-4 classes using conducting polymer electrodes; however tea liquors were well separated from the PCA plots employing the impedance data of both conducting polymer and metal electrodes. PMID:23035436

  19. Cinnamon Polyphenols Attenuate Neuronal Death and Glial Swelling in Ischemic Injury

    Science.gov (United States)

    Brain edema is a major complication associated with ischemic stroke and is characterized by a volumetric enlargement of the brain. Astrocyte swelling is a major component of brain edema. We investigated the protective effects of polyphenols isolated from green tea and cinnamon in C6 glial cultures s...

  20. Determination of volatile components of green, black, oolong and white tea by optimized ultrasound-assisted extraction-dispersive liquid-liquid microextraction coupled with gas chromatography.

    Science.gov (United States)

    Sereshti, Hassan; Samadi, Soheila; Jalali-Heravi, Mehdi

    2013-03-01

    Ultrasound assisted extraction (UAE) followed by dispersive liquid-liquid microextraction (DLLME) was used for extraction and preconcentration of volatile constituents of six tea plants. The preconcentrated compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). Totally, 42 compounds were identified and caffeine was quantitatively determined. The main parameters (factors) of the extraction process were optimized by using a central composite design (CCD). Methanol and chloroform were selected as the extraction solvent and preconcentration solvent, respectively .The optimal conditions were obtained as 21 in for sonication time; 32°C for temperature; 27 L for volume of extraction solvent and 7.4% for salt concentration (NaCl/H(2)O). The determination coefficient (R(2)) was 0.9988. The relative standard deviation (RSD %) was 4.8 (n=5), and the enhancement factors (EFs) were 4.0-42.6. PMID:23375769

  1. Comment on Chinese tea culture

    Institute of Scientific and Technical Information of China (English)

    王俊人

    2015-01-01

    Drinking tea has become a part of people's daily life. Tea culture as the crystallization of Chinese tradition has a long history. China is the homeland of tea. Tea as one of important exports has made a great contribution to economic develop-ment of Ancient China. Tasting tea is a unique culture of China. The tea quality, teahouse’s atmosphere and tea wares are all considered to be very important when tasting. With a long time of the habit of drinking tea, Chinese people have given tea the special spirit:clearness, respect, joy and truthfulness.

  2. Trace Elements in Norwegian and Polish Tea Infusions: Determined by High-Resolution Inductively Coupled Plasma Mass Spectrometry (HR ICP-MS) and Ion Selective Electrode (ISE)

    OpenAIRE

    Kronborg, Anne Ingelill Engvik

    2013-01-01

    AbstractTea from the plant Camellia sinensis is the world’s most popular non-alcoholic beverage, next to water. Tea can be divided into six types, and the most popular teas are black tea, green tea and oolong. In this study infusions of 85 Norwegian and Polish tea samples were analysed to investigate the content of trace elements. 40 samples from Norwegian supermarkets, 30 from the local tea and coffee shop and 15 Polish samples were infused for 5 minutes with ultrapure boiling water (1 gram ...

  3. Lipid oxidation degree and antioxidant activity of several polyphenolic extracts in bovine meat during storage

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    Ilir Lloha

    2013-12-01

    Full Text Available Extracts of vegetable origin are used widely nowdays in the food industry in the role of antioxidants, especially in the meat processing industry and in the industry of its byproducts. Subject of this study have been bovine meat samples, which have been subjected to polyphenolic extracts, such as those from: tea, rosemary and oregano conserved in a timeframe of 1, 4, 7 and 10 days. TBA (thiobarbituric acid assay shows that polyphenolic extracts tend to increase oxidative endurance of meat sample, while DPPH assay shows the level of antioxidant activity. Lipids oxidation degree and antioxidant activity of the samples of bovine meat treated with rosemary, oregano and tea polyphenolic extracts is lower than the control samples either in treated or not treated in 85°C samples. The samples which have been subjected to tea polyphenolic extract shows a lower lipid oxidation degree and a higher antioxidant activity compared not only to control samples but also to the samples treated with the other polyphenolic extracts. Lipid oxidation degree and antioxidant activity results greater in temperature treated samples compared to those in raw state.

  4. Lipid oxidation degree and antioxidant activity of several polyphenolic extracts in porcine meat during storage

    Directory of Open Access Journals (Sweden)

    ILIR LLOHA

    2014-03-01

    Full Text Available Extracts of vegetable origin are used widely nowdays in the food industry in the role of antioxidants, especially in the meat processing industry and in the industry of its byproducts. Subject of this study have been porcine meat samples, which have been subjected to polyphenolic extracts, such as those from: tea, rosemary and oregano conserved in a timeframe of 1, 4, 7 and 10 days. TBA (thiobarbituric acid assay show that polyphenolic extracts tend to increase oxidative endurance of meat sample, while DPPH assay shows an increased level of antioxidant activity. Lipids oxidation degree and antioxidant activity of the samples of porcine meat treated with rosemary, oregano and tea polyphenolic extracts is lower than the control samples either in treated or not treated in 85°C samples. The samples which have been subjected to tea polyphenolic extract show a lower lipid oxidation degree and a higher antioxidant activity compared not only to control samples, but also to the samples treated with other polyphenolic extracts. Lipid oxidation degree and antioxidant activity result are greater in temperature treated samples compared to those in raw state.

  5. Structural Properties of Green Tea Catechins.

    Science.gov (United States)

    Botten, Dominic; Fugallo, Giorgia; Fraternali, Franca; Molteni, Carla

    2015-10-01

    Green tea catechins are polyphenols which are believed to provide health benefits; they are marketed as health supplements and are studied for their potential effects on a variety of medical conditions. However, their mechanisms of action and interaction with the environment at the molecular level are still not well-understood. Here, by means of atomistic simulations, we explore the structural properties of four green tea catechins, in the gas phase and water solution: specifically, (-)-epigallocatechin-3-gallate, which is the most abundant, (-)-epicatechin-3-gallate, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, and (-)-epigallocatechin. We characterize the free energy conformational landscapes of these catechins at ambient conditions, as a function of the torsional degrees of freedom of the pholyphenolic rings, determining the stable conformers and their connections. We show that these free energy landscapes are only subtly influenced by the interactions with the solvent and by the structural details of the polyphenolic rings. However, the number and position of the hydroxyl groups (or their sustituents) and the presence/absence of the galloyl moiety have significant impact on the selected catechin solvation shells and hydrogen bond capabilities, which are ultimately linked to their ability to interact with and affect the biological environment. PMID:26369298

  6. GREEN TEA: A MAGICAL HERB WITH MIRACULOUS OUTCOMES

    Directory of Open Access Journals (Sweden)

    Anand Jigisha

    2012-05-01

    Full Text Available Green tea in its purest and most unadulterated form has always influenced human health from generations and day by day scientific evidences throughout the world are making people aware of health benefits associated with this herbal drink. Though Green Tea is not officially recognized as a medical agent, it is one of the most researched plant-based remedies whose possible benefits include promotion of cardio-vascular health, cancer prevention, skin protection, and antioxidant activity, to fight high cholesterol levels, infection, impaired immune function, diarrhoea, fatigue and many more. Laboratory findings have revealed that notable health benefit of green tea is its powerful antioxidants potential which at the molecular level, helps prevent cellular damage from certain oxidation reactions in the body. The credit for their useful antioxidant property lies with their huge collection of chemical substances called polyphenols and catechins make the major contribution of them. Though catechins have been found in other plants derivatives such as grapes, pomegranates, those found in tea have proven to be the most effective antioxidants known. The catechins epigallocatechin gallate (EGCG is found in the greatest concentration and most studied for its health benefits. There is an urgent need to check the efficacy, safety and translational guidelines for a green tea to be used as safe, effective drug. The main objective of this review is to enlighten some recent facts with relevance to the current status and advance in green tea benefits.

  7. Effect of Cinnamon Tea on Postprandial Glucose Concentration

    Science.gov (United States)

    Bernardo, Maria Alexandra; Silva, Maria Leonor; Santos, Elisabeth; Moncada, Margarida Maria; Brito, José; Proença, Luis; Singh, Jaipaul; de Mesquita, Maria Fernanda

    2015-01-01

    Glycaemic control, in particular at postprandial period, has a key role in prevention of different diseases, including diabetes and cardiovascular events. Previous studies suggest that postprandial high blood glucose levels (BGL) can lead to an oxidative stress status, which is associated with metabolic alterations. Cinnamon powder has demonstrated a beneficial effect on postprandial glucose homeostasis in animals and human models. The purpose of this study is to investigate the effect of cinnamon tea (C. burmannii) on postprandial capillary blood glucose level on nondiabetic adults. Participants were given oral glucose tolerance test either with or without cinnamon tea in a randomized clinical trial. The data revealed that cinnamon tea administration slightly decreased postprandial BGL. Cinnamon tea ingestion also results in a significantly lower postprandial maximum glucose concentration and variation of maximum glucose concentration (p < 0.05). Chemical analysis showed that cinnamon tea has a high antioxidant capacity, which may be due to its polyphenol content. The present study provides evidence that cinnamon tea, obtained from C. burmannii, could be beneficial for controlling glucose metabolism in nondiabetic adults during postprandial period. PMID:26258147

  8. Imidacloprid in processed tea and tea liquor

    Institute of Scientific and Technical Information of China (English)

    SANYAL N.; HAZRA D.; PAL R.; SOMCHAUDHURY A.K.; CHOWDHURY A.

    2006-01-01

    Imidacloprid is a newly introduced broad-spectrum chloronicotinyl insecticide and will find its way in agricultural production, particularly in Asia. However, information on the fate of imidacloprid in crop plants is lacking. The degradation of imidacloprid in processed CTC tea and tea liquor was investigated in the present study in which imidacloprid was applied at recommended application rate (30.0 g a.i./ha) and twice the recommended application rate (60.0 g a.i./ha) for three consecutive seasons. Imidacloprid was rapidly dissipated in processed tea following first order reaction kinetics at all application rates and had half-lives of 0.91~1.16 d with the residue in tea liquor found to be below detectable limit on 3rd day samples. The study revealed that imidacloprid is safe for human consumption and will not pose any residual toxicity problem.

  9. Tea: age-old beverage as an effective cancer chemopreventive agent

    Directory of Open Access Journals (Sweden)

    Jasmine George

    2011-12-01

    Full Text Available Cancer is the major public health problem, causing approximately 7 million deaths every year worldwide. The existing treatment approaches and surgical techniques have not been able to cope effectively with this dreaded disease. Because of this, the concept of chemoprevention is now considered a valid approach to reduce the incidence of cancer. There is convincing epidemiological and experimental evidence to show that dietary polyphenolic plant-derived compounds have cancer preventive properties. Based on evidence from in vitro, in vivo data and epidemiological studies, tea has received considerable attention over recent years for reducing the risk of several cancers. Much of the cancer preventive effects of tea, and in particular green tea, appear to be mediated by the polyphenols they contain. In addition to inhibiting mutagenesis and proliferation, tea is relatively non-toxic, is low cost, and can be taken orally or as a part of the daily diet. Therefore it is logical that future clinical studies should focus on examining the efficacy of tea and its active constituents, such as epigallocatechin- 3-gallate (EGCG and theaflavins (TFs, in chemoprevention as an alternative to pharmacological agents. In this review, we address the use of tea and its constituents for the prevention and treatment of cancer. Further mechanistic and dose-response studies will help us to understand the effects of tea consumption on human carcinogenesis.

  10. Factors affecting tea consumption pattern in an urban society in Isfahan, Iran

    Science.gov (United States)

    Rezaee, Elahe; Mirlohi, Maryam; Hassanzadeh, Akbar; Fallah, Azizolah

    2016-01-01

    Objective: To determine the tea consumption pattern in an urban society in Isfahan. Methods: A descriptive, cross-sectional survey was carried out using questioner among 664 households who were selected by two steps cluster sampling. A questionnaire was developed including demographic questions, information on the quantity of tea intake by every member of the family, tea type, and tea brand preference. Results: The individuals who prioritized black tea as their 1st choice were 90.1% of the total surveyed community. The average amount of tea consumed was calculated as 1243 ± 530 ml/day. The individuals who were between 36 and 45 and 0.05). Brand preference of tea showed that the individuals who prioritized Ahmed brand as their 1st choice constituted 44% of the total participants. Conclusion: Tea consumption pattern in an Iranian sample is specific. Despite of the huge evidence on the green tea benefits, this type of tea has very low popularity and among the available black tea brands, the imported ones are the most preferred.

  11. Antimicrobial activity of kombucha made from Rtanj tea

    Directory of Open Access Journals (Sweden)

    Cvetković Dragoljub D.

    2005-01-01

    Full Text Available Kombucha is a beverage with special therapeutic properties produced by the metabolic activity of yeasts and acetic acid bacteria in sweetened black tea (traditional cultivation medium. The antimicrobial activity of kombucha (for consumption made from black tea and Rtanj tea, as well as particular control samples, was examined by the modified disc diffusion method. Salmonella enteritidis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa Staphylococcus aureus, Bacillus sp., Sarcina lutea, Penicillium aurantiogriseum, Aspergilus niger, Aspergilus flavus, Rhodotorula sp. Candida pseudotropi-calis and Saccharomyces cerevisae have been used as test organisms. Acetic acid and kombucha samples show significant antimicrobial activity against all bacteria except Sarcina lutea. The other control samples (neutralized kombucha, tea and a "model sistem" show less bacteriostatic activity. Kombucha and acetic acid solution show borderline inhibitory activity against some moulds, while was no activity against yeasts.

  12. Symposium on Plant Polyphenols: Nutrition, Health and Innovations, June 2009.

    Science.gov (United States)

    Chang, Ann S; Yeong, Boon-Yee; Koh, Woon-Puay

    2010-04-01

    Reported here is a summary of the proceedings of the Symposium on Plant Polyphenols: Nutrition, Health and Innovations, which was cosponsored by the Southeast Asia Region branch of the International Life Sciences Institute and the Nutrition Society of Malaysia in Kuala Lumpur, Malaysia, June 22-23, 2009. The symposium provided a timely update of research regarding the protective effects of polyphenols in chronic diseases, such as cardiovascular disease and cancer, as well as the development of innovative polyphenol-containing food products with enhanced nutritive and health properties. Presentations covered polyphenols from a wide range of food sources such as tea, coffee, nuts and seeds, cocoa and chocolate, soy, and Asian fruits, vegetables, and spices. The symposium was attended by a large and diverse group of nutritionists, dietitians, researchers and allied health professionals, as well as management, research and development, and marketing personnel from the food and beverage industry. Their enthusiastic participation was a testament to the increasing awareness and interest in polyphenols in the prevention and control of chronic diseases. Presented here are some of the highlights and important information from the symposium. PMID:20416020

  13. Polyphenols: Potential Future Arsenals in the Treatment of Diabetes.

    Science.gov (United States)

    Solayman, Md; Ali, Yousuf; Alam, Fahmida; Islam, Md Asiful; Alam, Nadia; Khalil, Md Ibrahim; Gan, Siew Hua

    2016-01-01

    Diabetes mellitus (DM) is one of the most common endocrine metabolic disorders. In addition to exercise and diet, oral anti-diabetic drugs have been used as a part of the management strategy worldwide. Unfortunately, none of the conventional anti-diabetic drugs are without side effects, and these drugs pose an economic burden. Therefore, the investigation of novel anti-diabetic regimens is a major challenge for researchers, in which nature has been the primary resource for the discovery of potential therapeutics. Many plants have been shown to act as anti-diabetic agents, in which the main active constituents are believed to be polyphenols. Natural products containing high polyphenol levels can control carbohydrate metabolism by various mechanisms, such as protecting and restoring beta-cell integrity, enhancing insulin releasing activity, and increasing cellular glucose uptake. Blackberries, red grapes, apricots, eggplant and popular drinks such as coffee, cocoa and green tea are all rich in polyphenols, which may dampen insulin resistance and be natural alternatives in the treatment of diabetes. Therefore, the aim of this review is to report on the available anti-diabetic polyphenols (medicinal plants, fruits and vegetables), their mechanisms in the various pathways of DM and their correlations with DM. Additionally, this review emphasizes the types of polyphenols that could be potential future resources in the treatment of DM via either novel regimens or as supplementary agents. PMID:26601968

  14. Polyphenols-rich natural products for treatment of diabetes.

    Science.gov (United States)

    Dragan, S; Andrica, F; Serban, Maria-Corina; Timar, R

    2015-01-01

    Currently, experimental and clinical evidences showed that polyphenols-rich natural products, like nutraceuticals and food supplements, may offer unique treatment modalities in type 2 diabetes mellitus (DM), due to their biological properties. Natural products modulate the carbohydrate metabolism by various mechanisms, such as restoring beta-cells integrity and physiology, enhancing insulin releasing activity, and the glucose using. Sea buckthorn berries, red grapes, bilberries, chokeberries and popular drinks like cocoa, coffee and green tea are all rich in polyphenols and may decrease the insulin response, offer in g a natural alternative of treatment in diabetes. Therefore, researches are now focused on potential efficacies of different types of polyphenols, including flavonoids, phenolic acids, lignans, anthocyans and stilbenes. Animal and human studies showed that polyphenols modulate carbohydrate and lipid metabolism, decrease glycemia and insulin resistance, increase lipid metabolism and optimize oxidative stress and inflammatory processes. It is important to understand the proper dose and duration of supplementation with polyphenols-rich extracts in order to guide effective therapeutic interventions in diabetic patients. PMID:25174925

  15. Kinetics and Mechanistic Studies on the Reaction between Cytochrome c and Tea Catechins

    Directory of Open Access Journals (Sweden)

    Lihua Wang

    2014-08-01

    Full Text Available Green tea is characterized by the presence of an abundance of polyphenolic compounds, also known as catechins, including epicatechin (EC, epigallocatechin (EGC, epicatechin gallate (EGC and epigallocatechin gallate (EGCG. In addition to being a popular beverage, tea consumption has been suggested as a mean of chemoprevention. However, its mode of action is unclear. It was discovered that tea catechins can react with cytochrome c. When oxidized cytochrome c was mixed with catechins commonly found in green tea under non-steady-state conditions, a reduction of cytochrome c was observed. The reaction rate of the catechins was dependent on the pH and the nature of the catechin. The pseudo-first order rate constant obtained increased in the order of EC < ECG < EGC < EGCG, which is consistent with previously reported superoxide reduction activities and Cu2+ reduction activities of tea catechins.

  16. Effect of Exogenous MJA Treatment of Tea Plants on the Growth of Geometrid Larvae

    Institute of Scientific and Technical Information of China (English)

    GUI Lian-you; CHEN Zong-mao; LIU Shu-sheng

    2005-01-01

    The effect of tea plant Camellia sinensis induced by exogenous methyl jasmonate (MJA) on lipoxygenase (LOX), polyphenol oxidase (PPO) and proteinase inhibitor (PI) activity in the leaves of tea plants, as well as the growth and midgut proteinase activity of the geometrid Ectropis obliqua larvae were studied. MJA significantly induced LOX, PPO and PI activity in leaves of tea plants. When geometrid larvae have fed on leaves of tea plants treated with MJA, the activities of the high alkaline trypsin-like enzyme and chymotrypsin-like enzyme in their midgut were significantly inhibited, but the activities of the low alkaline trypsin-like enzyme in their midgut were unaffected, leading to imbalance between different types of proteinase activity in the midgut of the larvae and in turn, the growth were inhibited. These chains of response may be an important mechanism of the direct resistance induced by MJA-treatment of tea plant on geometrid larvae.

  17. Effect of irradiation decontamination on the qualities of green tea

    International Nuclear Information System (INIS)

    The purpose of this study was to analyze the effects of irradiation on the main chemical components, such as heavy metal elements, pesticide residues as well as sensory qualities of green tea. The results indicated that irradiation had no significant impact on proteins, tea polyphenols, theine and heavy metal elements, slight differences in the contents of soluble sugar and amino acids. The content of cypermethrin reduced with the increase of irradiation dose. The color, liquor color, flavor and aroma of the tea decoction changed slightly when irradiated at the dose lower than 5 kGy. It was concluded that the optimal doses for the purpose of green team decontamination was at the range of 3-5 kGy according to the analysis of various quality factors. (authors)

  18. Imperial Tea Connoisseurs

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    HAVING ruled for 60 years, in 1795 Qing emperor Qianlong decided it was time to abdicate in favor of his son. His ministers tried to dissuade him, saying: "A country cannot manage for a single day without its monarch." The emperor promptly retorted, raising his cup of tea, "A monarch cannot function for a single day without tea." Qianlong had several reasons for abdicating and mentioned tea only as a red her-

  19. PENGARUH PENAMBAHAN LAKASE DARI JAMUR TIRAM PUTIH (Pleurotus ostreatus) TERHADAP AKTIVITAS ANTIOKSIDAN TEH HIJAU [Effect of The Addition of Laccase from White Oyster Mushroom (Pleurotus ostreatus) Towards Antioxidant Activity of Green Tea

    OpenAIRE

    Tagor M. Siregar1)*; A. Herry Cahyana2); Natalia

    2012-01-01

    Laccase is one of the enzymes that can be used in organic synthesis using aromatic compounds (polyphenols and aminofenol) as substrates. Polyphenol compound in green tea is flavan-3-ols or catechin which are susceptible to enzymatic reaction with laccase. In this research laccase isolated from white oyster mushroom was added into the green tea extract. Addition of laccase is expected to yield products with a higher antioxidant activity. Prior to it’s use, laccase activity was determined and h...

  20. Antioxidant properties of tea investigated by EPR spectroscopy.

    Science.gov (United States)

    Polovka, Martin; Brezová, Vlasta; Stasko, Andrej

    2003-10-01

    The antioxidant properties of green, black and mixed (fruit) tea samples of different origin were investigated by means of EPR spectroscopy. A six line EPR spectrum of solid tea samples indicates the presence of Mn(II) ions and it is superimposed with a sharp singlet line attributed to semiquinone radical species (Delta H(pp)=1 mT; g=2.0022). Antioxidant properties of aqueous tea extracts in H(2)O(2)/NaOH/dimethylsulfoxide system generating reactive radicals (*OH, O(2)*-), *CH(3)) were followed by spin trapping technique. In addition, antioxidant capacity of these samples was assessed using stable radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPOL). Typically, the highest antioxidant potential to terminate superoxide radicals was found in green teas, followed by black and fruity teas. The pro-oxidant activity of green teas evidenced by spin traps was promoted in samples with higher Mn(II) and ascorbic acid concentrations. Various sources of free radicals used in the antioxidant tests due to their specific action show different termination rates in the presence of the individual tea samples. PMID:14516911