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Sample records for bk post trasplante

  1. Diagnóstico y clasificación molecular del virus BK en receptores de trasplante renal

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    Riva, Omar; Cobos, Marisa; Raimondi, J. Clemente

    2010-01-01

    La infección primaria por virus BK ocurre durante la infancia permaneciendo latente en el tracto urogenital. En individuos que presentan alteraciones en la inmunidad celular, el virus se reactiva haciendo posible su detección en orina y sangre. En receptores de trasplante renal, la nefropatía producida por el virus BK puede llevar a la pérdida de la función del injerto. El virus BK es miembro de la familia Polyomaviridae, presenta un genoma de ADN circular doble cadena unido en forma covalent...

  2. Factores asociados y consecuencias clínicas de la anemia post trasplante renal

    OpenAIRE

    Mónica Freiberg; Carlos Chiurchiu; Raúl Capra; Andrea Eckhardt; Jorge de la Fuente; Walter Douthat; Javier de Arteaga; Pablo U. Massari

    2013-01-01

    Un porcentaje considerable de pacientes presentan anemia post trasplante renal. Su origen es multifactorial y sus principales etiologías dependen de la etapa post trasplante que se considere. Estudiamos en un grupo de 134 pacientes los factores asociados con anemia tardía (6 meses post trasplante) y sus implicaciones clínicas a mediano plazo. En el análisis de regresión múltiple, la duración de la oliguria post trasplante y el número de episodios de rechazo fueron las variables significativam...

  3. Factores asociados y consecuencias clínicas de la anemia post trasplante renal

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    Mónica Freiberg

    2013-04-01

    Full Text Available Un porcentaje considerable de pacientes presentan anemia post trasplante renal. Su origen es multifactorial y sus principales etiologías dependen de la etapa post trasplante que se considere. Estudiamos en un grupo de 134 pacientes los factores asociados con anemia tardía (6 meses post trasplante y sus implicaciones clínicas a mediano plazo. En el análisis de regresión múltiple, la duración de la oliguria post trasplante y el número de episodios de rechazo fueron las variables significativamente asociadas con esta complicación. La supervivencia del órgano mostró una diferencia significativa a los 36 meses entre los grupos (83% en los anémicos versus 96% de los no anémicos p < 0.01. No observamos diferencias en mortalidad o eventos cardiovasculares. Concluimos que la presencia de anemia al sexto mes post trasplante renal está independiente y significativamente asociada con factores que condicionan la masa renal funcionante que explicarían además la menor supervivencia del injerto renal observada en estos pacientes.

  4. [β-estradiol activates BK(Ca) in mesenteric artery smooth muscle cells of post-menopause women].

    Science.gov (United States)

    Cheng, Jun; Zeng, Xiao-Rong; Li, Peng-Yun; Lu, Ting-Ting; Tan, Xiao-Qiu; Wen, Jing; Yang, Yan

    2012-04-25

    The aim of the present study was to study the effect of β-estradiol (β-E(2)) on the large-conductance Ca(2+)-activated potassium (BK(Ca)) channel in mesenteric artery smooth muscle cells (SMCs). The mesenteric arteries were obtained from post-menopause female patients with abdominal surgery, and the SMCs were isolated from the arteries using an enzymatic disassociation. According to the sources, the SMCs were divided into non-hypertension (NH) and essential hypertension (EH) groups. Single channel patch clamp technique was used to investigate the effect of β-E(2) and ICI 182780 (a specific blocker of estrogen receptor) on BK(Ca) in the SMCs. The results showed the opening of BK(Ca) in the SMCs was voltage and calcium dependent, and could be blocked by IbTX. β-E(2) (100 μmol/L) significantly increased open probability (Po) of BK(Ca) in both NH and EH groups. After β-E(2) treatment, NH group showed higher Po of BK(Ca) compared with EH group. ICI 182780 could inhibit the activating effect of β-E(2) on BK(Ca) in no matter NH or EH groups. These results suggest β-E(2) activates BK(Ca) in mesenteric artery SMCs from post-menopause women via estrogen receptor, but hypertension may decline the activating effect of β-E(2) on BK(Ca).

  5. Obesidad, diabetes y trasplante

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    Anna Bach Pascual

    Full Text Available El trasplante renal es la mejor opción terapéutica en términos de supervivencia y calidad de vida para los pacientes con enfermedad renal crónica. Los pacientes trasplantados son cada vez más mayores y con más pluripatologia asociada. Los avances en inmunosupresión y en el tratamiento han hecho que los pacientes trasplantados fallezcan con el injerto renal funcionante. Existe un incremento de peso del 10% durante el primer año del trasplante, la obesidad se relaciona con la aparición de diabetes mellitus postrasplante. Con el objetivo de analizar la incidencia de obesidad y los factores de riesgo relacionados, en una cohorte de pacientes trasplantados renales realizamos un estudio observacional y prospectivo de estos pacientes, desde enero del 2014 hasta marzo del 2015, con seguimiento de 1 año. Obteniendo como resultado, que los grupos son homogéneos en sexo, tiempo en terapia renal sustitutiva pero distintos en edad. A partir de los 3 meses los pacientes con índice de masa corporal mayor a 25 presentaron mayor incidencia de diabetes mellitus post trasplante respecto al grupo de pacientes con índice de masa corporal inferior a 25.

  6. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible

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    E. Bulliorsky

    2002-12-01

    Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.

  7. al trasplante de órganos

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    María Ángeles Pérez San Gregorio

    2005-01-01

    Full Text Available El trasplante de órganos es una alternativa terapéutica para aquellos pacientes que tienen problemas graves en el funcionamiento de un órgano vital: corazón, hígado, pulmón, etc. Dada la relevancia de este tema, en el presente estudio teórico analizamos los principales problemas psicológicos asociados al trasplante de órganos. Concretamente, hemos tenido en cuenta los siguientes apartados: 1 Repercusiones psicológicas del trasplante de órganos en los pacientes: delirium, trastornos sexuales, trastornos del estado de ánimo, trastornos de ansiedad, fantasías sobre el donante, insatisfacción con la imagen corporal y otros trastornos; 2 variables psicosociales que influyen en las repercusiones psicológicas asociadas al trasplante de órganos: apoyo familiar, trastornos psicológicos en los familiares, hospitalización, expectativas pre y post-trasplante, estrategias de afrontamiento, duración de la enfermedad, etiología del trasplante, procedencia del órgano implantado y otras variables; 3 fases psicológicas en los trasplantados renales, hepáticos y cardíacos en función de diferentes periodos temporales de comparación; 4 calidad de vida en los trasplantados y variables que la predicen: adherencia terapéutica, hospitalización, apoyo familiar, expectativas hacia el trasplante y etiología del trasplante; y 5 repercusiones psicológicas del trasplante de órganos en los familiares, teniendo en cuenta la influencia que ejerce el apoyo social sobre la salud mental de estos familiares.

  8. Short-tem Post Renal Trasplant Follow-up at Madinah Al Munawarah

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    Bernieh Bassam

    1999-01-01

    Full Text Available We reviewed the records of the renal transplant patients followed at our hospital to determine short-term outcome and complications. Sixty-five renal transplant patients, follow-up for two years were included in this study. Of these patients 40 (61.5% were males, 33 (50.7% were Saudis with mean age of 37.2 ± 11.7 years. Donors were living related (LRD in 23 (35%, living non-related (LNRD in 27 (42% and cadaveric (CAD in 15 (23%. Thirty-two transplants were carried out at Medinah, 21 in India and the rest in other centers inside Saudi Arabia. Immunosuppression was based on a triple therapy (Cyclosporin, Azathioprine, and Prednisone. At two years, 52 (80% patients were alive, with functioning graft in 31 (58%. Causes of death among 13 patients (11 LNRD & 2 CAD were infections in 7 (54%, immediate post transplant in three (22.7%, acute myocardial infarction in two (15.7%, CVA in one (7.6%. Complications encountered were acute rejection (23 episodes in 18 923.6% patients, infections in 19 (25%, chronic rejection in 16 (21.5%, surgical in 13 917.1%, diabetes mellitus in 5 (6.5% primary non-function in three (3.8% and Kaposi Sarcoma in two (2.4%. Twenty-six (81.25% out of 32 transplants performed in Madinah were functioning, four (12.5% patients returned to dialysis and two (6.25% patients died. Among the 21 transplants done in India 11 (52% patients died, six (28.6% returned to dialysis, and four (19.4% had function deteriorated in all patients. We conclude that despite limitations, results of renal transplantation carried out at Madinah are encouraging on short-term basis. Live related transplant has a very good outcome, while commercial transplantation carries poor prognosis.

  9. Intermittent losartan administration triggers cardiac post-conditioning in isolated rat hearts: role of BK2 receptors.

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    Luca Sgarra

    Full Text Available The angiotensin (Ang and bradykinin (BK tissue-system plays a pivotal role in post-conditioning, but the efficacy of angiotensin type 1 receptor (AT1R blockers (ARBs in post-ischemic strategies is still under investigation. We evaluated functional and morphological outcomes, together with activation of cytosolic RISK pathway kinases, in rat hearts subjected to losartan (LOS or irbesartan (IRB post-ischemic administration.Isolated rat hearts underwent 30 min ischemia and 120 min reperfusion. Post-conditioning was obtained by intermittent (10 s/each or continuous drug infusion during the first 3 min of reperfusion. Left ventricular end-diastolic pressure (LVEDP, left ventricular developed pressure (dLVP, coronary flow (CF, and left ventricular infarct mass (IM were measured together with the activation status of RISK kinases Akt, p42/44 MAPK and GSK3β.When compared to hearts subjected to ischemia/reperfusion (iI/R alone, continuous IRB or LOS administration did not significantly reduce total infarct mass (cIRB or cLOS vs. iI/R, p = 0.2. Similarly, intermittent IRB (iIRB was not able to enhance cardioprotection. Conversely, intermittent LOS administration (iLOS significantly ameliorated cardiac recovery (iLOS vs iI/R, p<0.01. Differences between iLOS and iIRB persisted under continuous blockade of AT2R (iLOS+cPD vs. iIRB+cPD, p<0.05. Interestingly, iLOS cardioprotection was lost when BK2R was simultaneously blocked (iLOS+cHOE vs. iI/R, p = 0.6, whereas concurrent administration of iBK and iIRB replicated iLOS effects (iIRB+iBK vs. iLOS, p = 0.7. At the molecular level, iIRB treatment did not significantly activate RISK kinases, whereas both iLOS and iBK treatments were associated with activation of the Akt/GSK3β branch of the RISK pathways (p<0.05 vs. iI/R, for both.Our results suggest that intermittent losartan is effective in mediating post-conditioning cardioprotection, whereas irbesartan is not. The infarct mass reduction by intermittent

  10. Evolucion de niños post-trasplante hepático luego del primer año de sobrevida Evolution of children one year post liver transplant

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    Miriam Cuarterolo

    2005-10-01

    Full Text Available El trasplante hepático constituye la única alternativa terapéutica para numerosas enfermedades hepáticas avanzadas. En el seguimiento post-trasplante pueden observarse complicaciones de diversa gravedad. El objetivo de este estudio fue analizar la evolución a largo plazo de los pacientes trasplantados con un seguimiento mayor de un año post quirúrgico. En el período 11/92-11/01 se realizaron 264 trasplantes en 238 pacientes; incluyéndose en este trabajo 143 niños con más de un año post-trasplante. La mediana de edad ± DS fue de 5.41 ± 5.26 años (r: 0.58-21.7 años. Catorce (9.79% fueron retrasplantados. Todos los pacientes recibieron ciclosporina (inmunosupresión primaria. Las indicaciones de trasplante fueron: falla hepática fulminante (n: 50; atresia de vías biliares (n: 38; cirrosis (n: 37; colestasis crónica (n: 13 y otras (n: 5. Las indicaciones de retrasplante fueron: cirrosis biliar (n: 7; trombosis de la arteria hepática (n: 4 y rechazo crónico (n: 3. Fueron utilizados injertos reducidos en 73/157 trasplantes(14 donantes vivos relacionados y 11 biparticiones hepáticas. La sobrevida global fue de 93%, paciente y 86%, injerto. El retrasplante y el injerto reducido fueron las variables de mayor significación para el aumento del riesgo de muerte. El déficit de talla y masa ósea se recuperó antes de los 3años post-trasplante. La incidencia de síndrome linfoproliferativo fue del 7.69%. Se diagnosticó hepatitis B de novo en 7 pacientes (4.8%. El riesgo social no afectó la sobrevida. La prevención, detección y tratamiento precoz de las complicaciones en el seguimiento a largo plazo permitió mejorar la evolución de los pacientes.Orthotopic liver transplantation is the only definitive mode of therapy for children with end-stage liver disease. However, it remains challenging because of the necessity to prevent long-term complications. The aim of this study was to analyze the evolution of transplanted patients with

  11. Infección por virus BK en paciente pediátrico trasplantado renal BK virus infection in a pediatric renal transplant recipient

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    R. Bonaventura

    2005-09-01

    Full Text Available El poliomavirus humano BK causa infección primaria asintomática en la niñez, estableciendo latencia principalmente en el tracto urinario. En individuos con alteración en la inmunidad celular se puede producir su reactivación desencadenando patología a nivel renal. Por estas razones es particularmente importante en la población pediátrica trasplantada renal, en la que puede producir la infección primaria cuando el paciente está inmunosuprimido. En nuestro trabajo se realizó el seguimiento de un paciente de 5 años trasplantado renal en octubre de 2003 que 45 días post-trasplante sufrió un deterioro del órgano injertado. Desde la fecha del trasplante hasta junio de 2004 se produjeron 3 episodios de alteración en la función renal, durante los cuales se analizaron muestras de sangre, orina, biopsia renal y líquido de linfocele. Para el diagnóstico difererencial entre rechazo agudo versus causa infecciosa se emplearon técnicas de detección para los virus BK, CMV y ADV, además del estudio citológico del tejido renal. Los resultados obtenidos junto con la clínica del paciente indican un probable caso de infección por BK. La importancia de realizar el diagnóstico diferencial entre rechazo agudo y la infección por BK radica en que la conducta en cuanto a la terapia inmunosupresora es opuesta en cada caso.BK Human Polyomavirus causes an asymptomatic primary infection in children, then establishing latency mainly in the urinary tract. Viral reactivation can lead to renal pathology in individuals with impaired cellular immune response. This is particularly important in pediatric transplant recipients, who can suffer a primary infection when immunosupressed. We followed up the case of a 5 years old patient who received a renal transplant in October 2003, and presented damaged graft 45 days after the intervention. The patient suffered 3 episodes of renal function failure between October 2003 and June 2004. Blood, urine, renal biopsy

  12. TRASPLANTE PULMONAR: ESTADO ACTUAL

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    María Teresa Parada C., DRA.

    2015-05-01

    Los desafíos en la actualidad son el déficit de órganos, que lleva aumento de lista de espera por lo que se han desarrollado técnicas de optimización de injertos, la extensión de la edad de los candidatos a trasplante y se mantiene sin grandes modificaciones la disfunción crónica de injerto.

  13. Aplasia pura de serie roja post-trasplante alogeneico de células progenitoras hematopoyeticas ABO incompatible Pure red cell aplasia after ABO incompatible bone marrow transplantation

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    E. Bulliorsky

    2002-12-01

    Full Text Available El trasplante alogeneico de células progenitoras hematopoyéticas (TCPH con incompatibilidad ABO entre el donante y el receptor puede en ocasiones asociarse a trastornos en la progenie eritroide desarrollada a partir de la médula ósea trasplantada, caracterizado por un funcionamiento tardío, inadecuado e incompleto de la misma. En este contexto, la aplasia pura de serie roja es la complicación más severa. Se han intentado tratamientos para la aplasia pura de serie roja post-TCPH con eritropoyetina o plasmaféresis, con relativo éxito. Algunos autores han informado también la utilización de globulina antilinfocitaria, asumiendo que dicha aplasia selectiva de la serie roja en la médula ósea trasplantada es mediada por un mecanismo inmune. En este trabajo se describe un paciente portador de una leucemia aguda en quien se realizó un TCPH alogeneico (ABO incompatible con su donante. Teniendo niveles bajos de aglutininas contra el grupo sanguíneo de la donante, desarrolló una aplasia pura de serie roja post - TCPH. La misma no mejoró con tratamiento con eritropoyetina o con un refuerzo de progenitores hematopoyéticos de sangre periférica de la misma donante (boost, resolviéndose totalmente luego de un tratamiento exitoso con globulina antilinfocitaria de origen equino.ABO incompatibility in allogeneic bone marrow transplantation may be associated with incomplete or delayed erythroid engraftment, being pure red cell aplasia (PRCA the most severe complication in this setting. Attempts for the treatment of PRCA have been made with erythropoietin or with plasmapheresis with relative success, and some authors have reported the reversibility of PRCA with antilymphocyte globulin (ALG or ATG, based on the assumption that PRCA might be immunologically mediated. We report herewith a patient with acute leukemia who developed post - BMT pure red cell aplasia. His sibling donor (sister was HLA identical and ABO incompatible, having low agglutinin

  14. Adaptación psicosocial de los adolescentes pos-trasplante renal, según la teoría de Roy Adaptação psicossocial do adolescente pós-trasplante renal segundo a teoria de Roy Psicosocial adaptation of post-renal transplanted adolescents according to Roy Theory

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    Ana Luisa Brandão de Carvalho Lirax

    2005-03-01

    Full Text Available El objetivo de este trabajo fue conocer las repercusiones psicosociales del adolescente después del trasplante renal y los mecanismos de adaptación utilizados en su nueva condición de vida. Es un estudio cualitativo, mediante entrevistas estructuradas. Fueron entrevistados diecinueve adolescentes que estaban en acompañamiento en dos hospitales públicos de Ceará-Brasil, después de la aprobación del proyecto por parte del Comité de Ética de las Instituciones y obtención del consentimiento libre e informado. Los resultados fueron analizados conforme a la teoría de la adaptación de Roy. Se identificaron problemas de adaptación, como: limitación física, vergüenza, miedo al rechazo e impotencia. Sin embargo, esos adolescentes pudieron superar tal situación y mejorar la adaptación a su nueva condición. Concluimos que la teoría de Roy permitió un análisis más profundo de los adolescentes, permitiéndoles un tratamiento más eficaz y, consecuentemente, una mejor calidad de vida.É um estudo descritivo, com análise qualitativo. O objetivo deste trabalho foi conhecer as repercussões psicossociais do adolescente após o trasplante renal e os mecanismos adaptativos utilizados por estes na sua nova condição de vida. Foram entrevistados dezenove adolescentes que estavam em acompanhamento ambulatorial em dois hospitais públicos do Ceará-Brasil, após aprovação do projeto pelo Comitê de Ética das Instituições e obtenção do consentimento livre e esclarecido. Os resultados foram analisados conforme a teoria da adaptação de Roy. Foram identificados problemas de adaptação, como: limitação física, vergonha, medo de rejeição e impotência. Contudo, esses adolescentes puderam superar tal situação e melhorar a adaptação à sua nova condição. Concluímos que a teoria de Roy permitiu uma análise mais aprofundada desses adolescentes, permitindo lhes um tratamento mais eficaz e, conseqüentemente, uma melhor qualidade de

  15. Intervención psiquiátrica en programa de trasplantes

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    G. Octavio Rojas, Dr.

    2010-03-01

    Full Text Available En los últimos años la Psiquiatría de Enlace e Interconsulta ha experimentado un salto cualitativo, acercándose al espacio común del resto de las especialidades médicas: el hospital general. La participación de psiquiatras en programas clínicos específicos como las unidades de trasplantes de órganos sólidos tiene fundamentaciones técnicas precisas dada la correlación entre síntomas psíquicos y las características clínicas específicas de las enfermedades médicas que requieren de este procedimiento. Cada vez hay una mayor preocupación en los clínicos y cirujanos en relación a los factores psíquicos que obstaculizan la adherencia a los tratamientos e indicaciones médicas tanto antes como después de efectuado el trasplante. La población de pacientes pre y post-trasplantados exhiben elevadas tasas de cuadros psiquiátricos específicos que ameritan un tratamiento coordinado multidisciplinario. El objetivo de este trabajo es mostrar la experiencia inicial que estamos desarrollando en la Unidad de Trasplantes de Clínica Las Condes.

  16. Perfil antiinflamatorio del paricalcitol en el receptor de trasplante renal

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    Javier Donate-Correa

    2017-11-01

    Conclusiones: La administración del paricalcitol a receptores de trasplante renal se asocia con efectos beneficiosos sobre su estado inflamatorio, lo que podría asociarse a un potencial beneficio clínico.

  17. Trasplante pulmonar: experiencia en clínica las condes

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    C. María Teresa Parada, Dra

    2010-03-01

    Hace 10 años Clínica Las Condes inicia su programa de trasplante pulmonar el que ha sido pionero nacional en número de injertos realizados y resultados obtenidos a largo plazo. Se realiza una revisión de las indicaciones, complicaciones precoces y tardías, capacidad física posterior al trasplante y sobrevida alejada de los pacientes trasplantados de pulmón en nuestro programa.

  18. Factores de riesgo de mortalidad precoz del Trasplante Pulmonar

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    Kreis Esmendi, Germán

    2006-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada A pesar de la experiencia acumulada, la mortalidad postoperatoria temprana del trasplante pulmonar continua siendo elevada. Los factores asociados a este evento permanecen siendo controvertidos. Objetivo: Revisar la serie de trasplantes pulmonares realizados en el Hospital Vall d' Hebron para establecer la supervivencia acumulada e identificar los factores asociados con riesgo de mortalidad postoperatoria precoz. Pacient...

  19. KCNMA1 encoded cardiac BK channels afford protection against ischemia-reperfusion injury.

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    Ewa Soltysinska

    Full Text Available Mitochondrial potassium channels have been implicated in myocardial protection mediated through pre-/postconditioning. Compounds that open the Ca2+- and voltage-activated potassium channel of big-conductance (BK have a pre-conditioning-like effect on survival of cardiomyocytes after ischemia/reperfusion injury. Recently, mitochondrial BK channels (mitoBKs in cardiomyocytes were implicated as infarct-limiting factors that derive directly from the KCNMA1 gene encoding for canonical BKs usually present at the plasma membrane of cells. However, some studies challenged these cardio-protective roles of mitoBKs. Herein, we present electrophysiological evidence for paxilline- and NS11021-sensitive BK-mediated currents of 190 pS conductance in mitoplasts from wild-type but not BK-/- cardiomyocytes. Transmission electron microscopy of BK-/- ventricular muscles fibres showed normal ultra-structures and matrix dimension, but oxidative phosphorylation capacities at normoxia and upon re-oxygenation after anoxia were significantly attenuated in BK-/- permeabilized cardiomyocytes. In the absence of BK, post-anoxic reactive oxygen species (ROS production from cardiomyocyte mitochondria was elevated indicating that mitoBK fine-tune the oxidative state at hypoxia and re-oxygenation. Because ROS and the capacity of the myocardium for oxidative metabolism are important determinants of cellular survival, we tested BK-/- hearts for their response in an ex-vivo model of ischemia/reperfusion (I/R injury. Infarct areas, coronary flow and heart rates were not different between wild-type and BK-/- hearts upon I/R injury in the absence of ischemic pre-conditioning (IP, but differed upon IP. While the area of infarction comprised 28±3% of the area at risk in wild-type, it was increased to 58±5% in BK-/- hearts suggesting that BK mediates the beneficial effects of IP. These findings suggest that cardiac BK channels are important for proper oxidative energy supply of

  20. Biopsia Renal en receptores de Trasplante Renal: cuidados de enfermería y complicaciones

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    Esther Sanz Izquierdo

    Full Text Available Introducción: La biopsia renal percutánea es una herramienta fundamental para el manejo del paciente trasplantado renal. La prueba es primordial para detectar y/o prevenir cualquier disfunción en el injerto, siendo un procedimiento tanto diagnóstico como preconizador. Objetivo: Describir los cuidados de enfermería e identificar las complicaciones derivadas de la biopsia renal en los receptores de Trasplante Renal. Material y métodos: Estudio cuantitativo, descriptivo y transversal realizado en la Unidad de Trasplante Renal, Servicio de Nefrología, del 2008 al 2014. La población objeto de estudio son los receptores de Trasplante Renal (TR. La muestra está compuesta por 368 biopsias renales de seguimiento que ingresan para someterse a una biopsia renal. Los criterios de inclusión son ser mayores de 18 años, trasplantados y que han firmado el consentimiento informado. Se recogen datos sociodemográficos, clínico-asistenciales y complicaciones post-biopsia renal. Resultados: Desde 1980 hasta el 2014 se han llevado a cabo 1868 TR, de 2008 a 2014 se estudiaron 368 biopsias de seguimiento. Se monitoriza la Tensión Arterial y la coagulación pre biopsia. Tras el procedimiento, se controla la presencia de sangrado por micción y constantes vitales. Inicialmente el reposo absoluto era de 24 h, a partir de 2014 se reduce a 6 horas, recomendando reposo relativo al alta, las complicaciones fueron mínimas. Conclusiones: Los resultados indican que la biopsia renal es un procedimiento eficaz, con escasas complicaciones. Destacar el papel de enfermería en la detección precoz de complicaciones.

  1. Seguimiento del perfil proteico urinario en el trasplante renal

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    Facio, María Laura; Madalena, Leticia; Bacqué, María del Carmen; Idiarte, Laura; Pandolfo, Marcela; Angerosa, Margarita; Alejandre, Mariel; Fraind, Susana; Bresciani, Pablo; Pizzolato, Marco

    2010-01-01

    La nefropatía crónica del trasplante (NCT) se caracteriza por fibrosis intersticial y atrofia tubular, pero su etiología es diversa. El objetivo del trabajo fue evaluar el seguimiento cualitativo de proteínas urinarias en pacientes con más de seis años de trasplante renal y compararlo con parámetros de laboratorio y con biopsia renal. Se evaluaron 17 pacientes durante un año, a través de creatinina sérica, proteinuria y fraccionamiento proteico por electroforesis en geles de poliacrilamida (S...

  2. Capítulo 11. Paciente candidato a trasplante cardiaco

    Directory of Open Access Journals (Sweden)

    Juan E. Gómez

    2016-03-01

    Conclusión: La necesidad de trasplante cardiaco se plantea una vez se han agotado las terapias farmacológicas y no farmacológicas disponibles. Los candidatos potenciales debe ser remitidos de forma prioritaria para su evaluación y seguimiento en una clínica de falla cardiaca que cuente con esta alternativa.

  3. Certeza diagnóstica en la mortalidad de una población de pacientes con trasplante cardíaco

    Directory of Open Access Journals (Sweden)

    Marcos Amuchástegui

    2008-01-01

    Full Text Available RESUMENIntroducciónA pesar de que la morbimortalidad en el trasplante cardíaco ha sido motivo de extensoanálisis, la mayoría de los estudios y registros de mortalidad en pacientes trasplantados sebasan sobre datos clínicos. En la bibliografía existen comunicaciones aisladas de autopsiasen pacientes con trasplante cardíaco.ObjetivoDeterminar la importancia de la realización de estudios anatomopatológicos para el diagnósticode causa de muerte en un programa de trasplante cardíaco.Material y métodosSe incluyeron todos los pacientes con trasplante cardíaco fallecidos entre enero 1990 y enero2005. El diagnóstico definitivo de la causa de muerte fue corroborado por autopsia obiopsia de órgano sólido. Las causas de muerte evaluadas fueron falla precoz del injerto,rechazo celular, infección, enfermedad vascular del injerto, neoplasia y otros.ResultadosDurante el período en estudio 73 pacientes fueron sometidos a trasplante cardíaco; de ellos,fallecieron 31. Se obtuvieron 12 autopsias y 7 biopsias de órgano sólido que certificaron lacausa de muerte (61%. La causa de muerte más frecuente fue el rechazo celular mayor degrado III. En el 12,9%, la anatomía patológica difirió de la sospecha clínica de la causa demuerte.ConclusiónLa información clinicopatológica derivada de estudios post mortem es un indicador de nuestrarealidad asistencial y se constituye en un pilar fundamental para el conocimiento y elmanejo futuro de los pacientes trasplantados, por lo que consideramos que la realización deautopsias en estos pacientes es de vital importancia.REV ARGENT CARDIOL 2008;76:292-294.

  4. BK channel modulators: a comprehensive overview

    DEFF Research Database (Denmark)

    Nardi, Antonio; Olesen, Søren-Peter

    2008-01-01

    channels as a potentially attractive target, the design and synthesis of potent and selective BK modulators continue based on novel chemical ideas. A comprehensive overview of BK channel modulators is therefore timely and important to the current medicinal chemist for review, summary, and classification...

  5. Inmunidad humoral y trasplante renal posibilidades terapéuticas

    Directory of Open Access Journals (Sweden)

    P. Jacqueline Pefaur, Dra.

    2010-03-01

    Por otra parte y basado en el exitoso tratamiento del RAH, se ha planteado mejorar las expectativas de llegar a realizar un trasplante a los pacientes sensibilizados. Esto es posible conseguir aplicando protocolos de desensibilización que se basan en la utilización de las mismas drogas para tratar RAH, consiguiendo ampliar las posibilidades de trasplante. El éxito de éstas es relativo al tipo de protocolos y a la intensidad de la sensibilización. La sobrevida del injerto en esta situación es plausible en la gran mayoría de los casos, sin embargo existe riesgo de presentar rechazo agudo humoral, y más complejo aún es el hecho que la sobrevida a largo plazo de los injertos sigue siendo todavía desconocida.

  6. Inmunidad humoral y trasplante renal posibilidades terapéuticas

    OpenAIRE

    P. Jacqueline Pefaur, Dra.; M. Susana Elgueta, Dra.

    2010-01-01

    El trasplante renal es el tratamiento de elección para los pacientes con falla renal terminal. Las principales causas de pérdida de injertos son la muerte del paciente con injerto funcionante, especialmente de causa cardiovascular y la nefropatía crónica del injerto, con una pérdida crónica de injertos que resulta en un problema relevante. Dentro de las causas de nefropatía crónica destaca la causa inmunológica. Una de las causas de pérdida de injertos de origen inmunológico son los rechazos ...

  7. BK virus infection in a renal transplant Saudi child

    International Nuclear Information System (INIS)

    Maghrabi, M.; Marwan, D.; Osoba, Abimbola O.

    2007-01-01

    BK human polyomavirus (BKV) causes an asymptomatic primary infection in children, but later, establishes latency mainly in the urinary tract. Virus-host interactions influencing persistence and pathogenicity are not well-understood. We present here a 12-year-old Saudi boy, who had renal transplant in Egypt. Seven months later, he was admitted to our Pediatric Nephrology Unit as a case of renal impairment. He developed BKV infection, diagnosed and successfully managed in our hospital. This case demonstrates the expanding clinical importance of BKV in a post renal transplant patient. This virus can be detected in transitional cells in the urine (decoy cells) using cytology. Testing for BKV deoxyribonucleic acid in urine and blood is an early detection assay, and can be used as a screening test in the early stages. The early reduction of immunosuppression can improve the prognosis. No specific antiviral treatment has been established yet. This is the first report of detecting BK virus in a Saudi post-transplant child in urine and blood specimens by using polymerase chain reaction. (author)

  8. Donación y trasplante de órganos y tejidos

    OpenAIRE

    Caicedo Rusca, Luis Armando; Alex, Castro; Ordóñez, Vasco; Jurado, Fátima; Echeverry, Patricia; Fundación Valle de Lili

    2010-01-01

    Donación y trasplante de órganos y tejidos/¿Crees en la vida después de la vida?/Qué dice la ley colombiana/Tipos de donantes de órganos/el dónate de órganos una urgencia de vida o muerte/ Quién puede ser donante de órganos/ Trayectoria de los trasplantes en la Fundación Valle del Lili/Preguntas y respuestas/ ¿Cuáles son los órganos que más se requieren para trasplante?/¿Qué trasplantes se realizan en Colombia?/¿Cómo hacen para enterarse que una persona es donante de órganos si el paciente es...

  9. TRASPLANTE SIMULTÿNEO DE PANCREAS-RIÿÿN. CONCEPTOS ACTUALES Y EXPERIENCIA EN CLÿNICA LAS CONDES

    Directory of Open Access Journals (Sweden)

    DR. Mario Ferrario B.

    2016-03-01

    Resultados: De los 16 enfermos, 9 de ellos fueron hombres, la edad promedio fue 38,7 años al momento del trasplante, el tiempo promedio de diabetes fue 23,5+/-7.3 años. Todos los injertos pancreáticos fueron anastomosados a los vasos ilíacos comunes derechos en forma término-terminal y el duodeno fue anastomosado en 8 casos a la vejiga y en los últimos 8 al íleon. La sobrevida de los pacientes a 10 años fue del 81%, del injerto de páncreas el 82% y del injerto renal el 65%. La complicación post operatoria más importante fue sepsis, causando la muerte en 2 pacientes. Y entre las complicaciones de tipo inmunológico, 8 pacientes presentaron rechazo agudo, siendo manejados con terapia esteroidal de rescate o timo globulina.

  10. Absorption of 249Bk from the gastrointestinal tract

    International Nuclear Information System (INIS)

    Zalikin, G.A.; Nisimov, P.G.

    1988-01-01

    In experimets with albino mongrel female rats a study was made of the absorption of 249 Bk from the gastrointestinal tract after a single per os administration. The bulk of 249 Bk (96 per cent) administered either intravenously or per os was mainly deposited in the skeleton and liver. The value of 249 Bk absorption from the gastrointestinal trat by days 4 and 8 following administration was 0.05 per cent

  11. Revisión actualizada sobre el trasplante de órganos: estudio de un caso

    OpenAIRE

    Andreu Periz, Lola; Force Sanmartín, Enriqueta

    2005-01-01

    El trasplante de órganos supone una de las hazañas más importantes de la ciencia moderna. Tras más de 50 años de experiencia, los trasplantes de órganos y tejidos, junto a las nuevas terapias celulares, abren un amplio abanico de posibilidades y son una de las opciones terapéuticas más válidas para algunas enfermedades que hasta hace poco no tenían solución. El trasplante de órganos permite la supervivencia de muchos pacientes y supone una notable mejoría de la calidad de vida. El conocimient...

  12. Viviendo con los pacientes el sueño del trasplante: una experiencia enfermera

    Directory of Open Access Journals (Sweden)

    Ana Julia Carrillo Algarra

    2014-06-01

    Full Text Available El trasplante renal puede ser una solución para personas que sufren insuficiencia renal crónica. Durante este proceso es de vital importancia el cuidado de enfermería en las diferentes etapas a las que el paciente y su familia se ven enfrentados. Este es el relato biográfico de una enfermera con amplia experiencia en el cuidado de pacientes que han vivido el trasplante renal, que describe diferentes aspectos que se deben tener en cuenta en la relación del equipo interdisciplinario, con el paciente y la familia; a través del mismo narra las vivencias y los sentimientos que afloran en el diario vivir de su labor y comenta su opinión respecto al futuro de la Enfermería en trasplantes.

  13. BIOÉTICA, TRASPLANTE DE ÓRGANOS Y DERECHO PENAL EN COLOMBIA

    Directory of Open Access Journals (Sweden)

    Yolanda M. Guerra García

    2011-01-01

    Full Text Available Este artículo presenta los lineamientos del Derecho Penal que trascienden los conceptos de trasplantes de órganos y las implicaciones Bioéticas de los mismos. Del mismo modo se establecen algunos de los principales criterios relacionados con el debate bioético en torno al problema de la Donación y Trasplante de Órganos, problemas relacionados con temas como la Muerte Encefálica, el consentimiento informado, Justicia y Distribución, los Xenotrasplantes y la Clonación Embrionaria entre otros.

  14. Trasplante cardíaco pediátrico: pasado, presente y futuro

    Directory of Open Access Journals (Sweden)

    María-Teresa González-López

    2017-01-01

    Conclusión: El trasplante cardíaco pediátrico presenta óptimos resultados en nuestro medio, superponibles a series mundiales. Aunque el perfil de riesgo está incrementándose, los resultados actuales reflejan los avances en el manejo de estos pacientes.

  15. Trasplante después del Fontan. Aspectos quirúrgicos

    Directory of Open Access Journals (Sweden)

    Juan-Miguel Gil-Jaurena

    2016-09-01

    Conclusiones: Trasplantar a pacientes con Fontan previo es un reto. Podemos anticipar variantes en cada una de las 5 anastomosis previstas. Es recomendable obtener tejido extra del donante (aorta y arco, vena cava superior e innominada, pericardio. Los resultados pueden superponerse a otras series de trasplante cardiaco.

  16. Trasplante cardíaco: organización e indicaciones

    Directory of Open Access Journals (Sweden)

    José M. Revuelta

    2008-01-01

    La experiencia clínica está demostrando que las indicaciones y las contraindicaciones del trasplante cardíaco están en constante cambio, debido principalmente a los avances en los cuidados pre, per y postoperatorios, a los nuevos fármacos y otras novedosas alternativas terapéuticas. La clase funcional (NY HA del paciente es poco precisa para indicar el trasplante cardíaco, por lo que se precisa de otras determinaciones, como el consumo máximo de oxígeno: VO2 máx < 10 ml/kg/min conlleva un mortalidad hospitalaria elevada. Asimismo, la fracción de eyección ventricular izquierda disminuida (FEVI < 20% no debe considerarse como el indicador principal para establecer la indicación de trasplante, siendo necesario valorar otros factores de riesgo preoperatorios. En la última década, la experiencia ha puesto de manifiesto que algunas de las contraindicaciones absolutas, antes vigentes, no siempre deben desaconsejar el trasplante cardíaco.

  17. El cuidado en un programa de trasplante renal: un acompañamiento de vida

    Directory of Open Access Journals (Sweden)

    Ana Julia Carrillo-Algarra

    2015-01-01

    Full Text Available Objetivo: comprender las experiencias de cuidado de enfermeras integrantes de grupos de trasplante de la ciudad de Bogotá. Materiales y métodos: investigación cualitativa, utilizó la estrategia de grupos focales; la organización y el análisis de datos siguieron la propuesta de codificación de Janice M. Morse; participaron, previo consentimiento informado, 10 de 20 enfermeras de unidades de trasplante. Se realizó triangulación entre las integrantes del equipo investigador y la literatura relevante. Resultados: emergieron 6 ca- tegorías: 1 los múltiples significados del trasplante: cambio, pasión, satisfacción, esperanza, libertad, mayor cercanía, responsabilidad, compromiso, complejidad; 2 las redes de apoyo: familia, asociación de enfermeras, grupo de trabajo interdisciplinario, empresas pro- motoras de salud, industria farmacéutica; 3 implicaciones del día a día: relación cercana con el paciente y la familia, fortalecimiento de la autoestima profesional, estatus dentro del grupo de trasplante, manejo de las cargas; 4 trayectorias del cuidado profesiona l: acom- pañamiento de la enfermera a través del ciclo vital del paciente y posibilidad de identificar necesidades por la estrecha relac ión con él; 5 el perfil de la enfermera: carácter, conocimiento, compromiso, disponibilidad permanente y capacidad de gestión; 6 contradi cciones del trasplante: rechazo al injerto, sufrimiento, desesperanza, sistema de salud; para enfermería, las agotadoras jornadas de tr abajo.

  18. Agnoprotein Is an Essential Egress Factor during BK Polyomavirus Infection

    Directory of Open Access Journals (Sweden)

    Margarita-Maria Panou

    2018-03-01

    Full Text Available BK polyomavirus (BKPyV; hereafter referred to as BK causes a lifelong chronic infection and is associated with debilitating disease in kidney transplant recipients. Despite its importance, aspects of the virus life cycle remain poorly understood. In addition to the structural proteins, the late region of the BK genome encodes for an auxiliary protein called agnoprotein. Studies on other polyomavirus agnoproteins have suggested that the protein may contribute to virion infectivity. Here, we demonstrate an essential role for agnoprotein in BK virus release. Viruses lacking agnoprotein fail to release from host cells and do not propagate to wild-type levels. Despite this, agnoprotein is not essential for virion infectivity or morphogenesis. Instead, agnoprotein expression correlates with nuclear egress of BK virions. We demonstrate that the agnoprotein binding partner α-soluble N-ethylmaleimide sensitive fusion (NSF attachment protein (α-SNAP is necessary for BK virion release, and siRNA knockdown of α-SNAP prevents nuclear release of wild-type BK virions. These data highlight a novel role for agnoprotein and begin to reveal the mechanism by which polyomaviruses leave an infected cell.

  19. 67. Lecciones aprendidas del trasplante cardiohepático simultáneo

    Directory of Open Access Journals (Sweden)

    F. Gómez

    2010-01-01

    Full Text Available El trasplante simultáneo combinado de corazón e hígado es una opción de tratamiento en pacientes en fase final de la insuficiencia cardíaca y hepática. Desde su descripción inicial por Starzl en 1985, no ha sido un procedimiento muy expandido, siendo pocos y de escasa cuantía los registros que aparecen en la literatura. Presentamos dos hermanos de 51 y 54 años afectos de amiloidosis familiar en su variedad transtiretina amiloidea (ATTR (mutación gen E89K de la transtirretina, ambos con miocardiopatía restrictiva e hígado congestivo con ingresos previos por descompensación hepática e insuficiencia cardíaca. Tras el estudio pretrasplante cardíaco y hepático completo sin contraindicación al mismo, fueron incluidos en lista de trasplante cardíaco-hepático. Dado el grado avanzado de insuficiencia de ambos órganos, se consideró la opción de trasplante en un solo tiempo como única posibilidad. El trasplante fue llevado a cabo en dos fases, con el implante inicial cardíaco y, una vez terminada la circulación extracorpórea, se procedió a la fase hepática. Se plantean los problemas del trasplante en dos tiempos o simultáneo a partir de órganos de un solo donante, la ubicación en las listas de espera, la técnica quirúrgica, el momento de la administración de protamina, el cierre esternal pre o postrasplante hepático y el manejo intra y postoperatorio. Ambos pacientes presentaron un curso postoperatorio correcto, dándose de alta hospitalaria a los 20 y 27 días del trasplante con correcta función cardíaca y hepática. Los controles ambulatorios de ambos pacientes son favorables, mostrándose en clase funcional I y sin descompensaciones hepáticas.

  20. ADAPTACIÓN DEL MODELO ESPAÑOL DE GESTIÓN EN TRASPLANTE PARA LA MEJORA EN LA NEGATIVA FAMILIAR Y MANTENIMIENTO DEL DONANTE POTENCIAL

    Directory of Open Access Journals (Sweden)

    Neide da Silva Knihs

    2011-01-01

    Full Text Available El propósito del estudio fue comparar los datos relacionados a la formación y perfil de los coordinadores de trasplante de Brasil y España para evaluar oportunidades de mejoría en la negativa familiar y el mantenimiento del donante en nuestro país. Una minuciosa evaluación fue realizada, comparando los datos de formación del coordinador de trasplante, perfil del coordinador de trasplante y de la metodología de trasplante de cada país. En Brasil el promedio de horas de capacitación recibida antes de empezar a trabajar con trasplantes es de ocho horas, en España de 42 horas. En Brasil 22,9% de los coordinadores de trasplante son médicos y en España 95% son médicos. El tiempo que actúa el coordinador de trasplantes en Brasil es de nueve meses y 22 días y en España 60 meses. El Modelo de Gestión de la Organización Nacional de Trasplante, puede ser adaptado en Brasil, como una forma de mejoría en la formación del coordinador de trasplante y de cambiar las tasas por negativa familiar y por pérdidas de mantenimiento.

  1. Polyomavirus specific cellular immunity: from BK-virus-specific cellular immunity to BK-virus-associated nephropathy ?

    Directory of Open Access Journals (Sweden)

    manon edekeyser

    2015-06-01

    Full Text Available In renal transplantation, BK-virus-associated nephropathy has emerged as a major complication, with a prevalence of 5–10% and graft loss in >50% of cases. BK-virus is a member of the Polyomavirus family and rarely induces apparent clinical disease in the general population. However, replication of polyomaviruses, associated with significant organ disease, is observed in patients with acquired immunosuppression, which suggests a critical role for virus-specific cellular immunity to control virus replication and prevent chronic disease. Monitoring of specific immunity combined with viral load could be used to individually assess the risk of viral reactivation and virus control. We review the current knowledge on BK-virus specific cellular immunity and, more specifically, in immunocompromised patients. In the future, immune-based therapies could allow us to treat and prevent BK-virus-associated nephropathy.

  2. Profil Siswa Agresif dan Peranan Guru BK

    Directory of Open Access Journals (Sweden)

    Hafiz Hidayat

    2013-06-01

    Full Text Available Abstract: Teens have a positive and negative behavior. Negative behavior is not characteristic of normal adolescent development, adolescents are developing will exhibit positive behavior. One of the teens indicated negative behavior is aggressive behavior. It can be seen in SMA N 5 Padang is quarrelsome, like teasing, hitting, pinching her, saying rude, insulting. This study aimed to describe the type of aggressive behavior of students and the role of BK teachers to address students' aggressive behavior SMAN 5 Padang. This study used a descriptive approach. The study population was a high school student Padang N 5 340 people with a total sample amounted to 77 people. Questionnaire data collection techniques to use. Data were analyzed using Microsoft Office Excel 2007. The findings showed that students' views of the aggressive actions hurt people physically with a percentage of 35.32%, while the aggressive actions of the student views of hurting people verbally 41.30% and aggression seen from damaging and destroying property by 30 percent, 42%. This means that the presence of aggressive behavior in SMA N 5 Padang.

  3. Pelaksanaan Asas-Asas BK dalam Pelayanan BK (Ditinjau dari Persepsi Siswa

    Directory of Open Access Journals (Sweden)

    Yasinta Nur Miftakhul Jannah

    2015-09-01

    Full Text Available Penelitian ini didasarkan pada data dan fenomena yang ditemukan di lapangan yang menunjukkan rendahnya pelaksanaan asas-asas bimbingan dan konseling oleh konselor di sekolah. Penelitian ini bertujuan untuk mengetahui gambaran pelaksanaan asas-asas BK dalam pelayanan BK di SMA Negeri se-Kabupaten Pati (ditinjau dari persepsi siswa kelas XI Tahun Ajaran 2014/2015. Populasi penelitian ini adalah siswa kelas XI di SMA Negeri se-Kabupaten Pati. Pengambilan sampel penelitian dilakukan dengan menggunakan teknik proportionale homogen random sampling dengan sampel sebesar 307 siswa kelas XI. Metode pengumpulan data menggunakan skala psikologis dalam bentuk skala persepsi. Metode analisis data menggunakan statistik deskriptif persentase. Hasil dari penelitian menunjukkan, gambaran pelaksanaan asas-asas BK secara umum sudah masuk pada kategori baik dengan persentase sebesar 73,45%. Asas yang paling tinggi pelaksanaannya yaitu asas kegiatan dengan persentase sebesar 79,80%. Asas yang masih tergolong rendah persentase pelaksanaannya yaitu asas kekinian dengan persentase sebesar 68,80% dan asas alih tangan dengan persentase sebesar 69,10%. This research is based on data and phenomena found in a field that shows low  implementation of guidance and counseling principles by counselor at school. This research aims to find out the implementation of guidance and counseling principles in counseling services toward public senior high school throughout Pati Regency (reviewed from grade XI students’ perceptions Academic Year 2014/2015. Population of this research are grade XI public senior high school students throughout Pati Regency. Sampling in this research is done by using proportionale homogen random sampling technique with the number of samples 307 of grade IX. Collecting data method using psychological scale in the form of scale perception. Data analysis method which used is descriptive percentage. The result shows description of the implementation of

  4. Coronary arterial BK channel dysfunction exacerbates ischemia/reperfusion-induced myocardial injury in diabetic mice.

    Science.gov (United States)

    Lu, Tong; Jiang, Bin; Wang, Xiao-Li; Lee, Hon-Chi

    2016-09-01

    The large conductance Ca(2+)-activated K(+) (BK) channels, abundantly expressed in coronary artery smooth muscle cells (SMCs), play a pivotal role in regulating coronary circulation. A large body of evidence indicates that coronary arterial BK channel function is diminished in both type 1 and type 2 diabetes. However, the consequence of coronary BK channel dysfunction in diabetes is not clear. We hypothesized that impaired coronary BK channel function exacerbates myocardial ischemia/reperfusion (I/R) injury in streptozotocin-induced diabetic mice. Combining patch-clamp techniques and cellular biological approaches, we found that diabetes facilitated the colocalization of angiotensin II (Ang II) type 1 receptors and BK channel α-subunits (BK-α), but not BK channel β1-subunits (BK-β1), in the caveolae of coronary SMCs. This caveolar compartmentation in vascular SMCs not only enhanced Ang II-mediated inhibition of BK-α but also produced a physical disassociation between BK-α and BK-β1, leading to increased infarct size in diabetic hearts. Most importantly, genetic ablation of caveolae integrity or pharmacological activation of coronary BK channels protected the cardiac function of diabetic mice from experimental I/R injury in both in vivo and ex vivo preparations. Our results demonstrate a vascular ionic mechanism underlying the poor outcome of myocardial injury in diabetes. Hence, activation of coronary BK channels may serve as a therapeutic target for cardiovascular complications of diabetes.

  5. Funcionamiento familiar en situación de trasplante de órganos pediátrico

    Directory of Open Access Journals (Sweden)

    Elisa Kernde Castro

    2005-01-01

    Full Text Available El presente trabajo revisa recientes hallazgos y examina algunas cuestiones relacionadas a las implicaciones del trasplante de órganos en la infancia para las relaciones familiares. El trasplante de órganos pediátrico es un procedimiento que puede desestabilizar el funcionamiento familiar y puede influenciar la adaptación y manejo del niño con respecto a la enfermedad y tratamiento. Los estudios revisados muestran que se pueden producir cambios importantes en las relaciones familiares cuando se plantea la posibilidad de un trasplante de órganos, especialmente un aumento en el estrés de los padres y la aparición de conductas sobreprotectoras con el niño. Actualmente existen pocos estudios sobre el tema, pero las evidencias revelan que el funcionamiento familiar y la salud mental de los padres son aspectos importantes que pueden influenciar el bienestar psicológico del niño.

  6. Trasplante hepático pediátrico estudio descriptivo de la experiencia recogida por el grupo de trasplante pediátrico de clínica las condes y hospital luis calvo mackenna

    Directory of Open Access Journals (Sweden)

    M. Mario Uribe, Dr.

    2010-03-01

    Conclusión: Los resultados del THP en términos de sobrevida y complicaciones es similar a la experiencia de centros extranjeros, la cual es superior a las expectativas y calidad de vida de los pacientes sin trasplante. Las áreas de mayor desarrollo de nuestro centro y que presentan los mayores desafíos son el trasplante hepático en niños menores de 10 kilos, en falla hepática aguda, y el uso de donante vivo.

  7. BK Virus-Associated Nephropathy without Viremia in an Adolescent Kidney Transplant Recipient

    Directory of Open Access Journals (Sweden)

    Kraisoon Lomjansook, M.D.

    2017-09-01

    Full Text Available BK virus can reactivate in kidney transplant recipients leading to BK virus-associated nephropathy (BKVAN and allograft dysfunction. Pathogenesis begins with viral replication, follows by viruria, viremia and nephropathy. Screening tools recommended for viral detection are urine and blood BK viral load. Viremia has higher positive predictive value than viruria, thus several guidelines recommend using viremia to determine whether renal biopsy, a gold standard for diagnosis of BKVAN is needed. We present a 16-year-old boy who developed BKVAN five months after deceased donor kidney transplantation. He had increased serum creatinine with negative blood BK viral load. BK nephropathy was diagnosed in kidney graft biopsy. The urine showed BK viruria. Immunosuppressant was reduced and ciprofloxacin given. Viruria disappeared and repeated graft biopsy was normal 4 months later. BK viremia was negative through 1 year follow up. We conclude that BKVAN may occur even without viremia and BK viruria may be considered for screening tool.

  8. Urinary BK virus excretion in children newly diagnosed with acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Nahid Raeesi

    2012-01-01

    Conclusion: To demonstrate the role of BK virus in inducing ALL or increasing the number of relapses, prospective studies on larger scale of population and evaluating both serum and urine for BK virus are recommended.

  9. Seguridad de la vacuna de la gripe en el receptor de trasplante de órgano sólido

    OpenAIRE

    Bulnes Ramos, Ángel

    2016-01-01

    La infección por influenza en los pacientes receptores de trasplante de órgano sólido (TOS) se ha asociado con altas tasas de morbilidad y mortalidad, especialmente en los primeros tres meses tras el trasplante. La vacunación de la gripe se ha propuesto como una medida eficaz para la prevención de la infección en estos pacientes. La mayoría de los estudios realizados para evaluar la eficacia de la vacunación en este grupo de pacientes han reflejado una respuesta humoral óptima en el receptor ...

  10. Mecanismos sociales de coordinación en el Sistema Español de Trasplantes

    Directory of Open Access Journals (Sweden)

    Carmen De Pablos Heredero

    2013-01-01

    Full Text Available El proceso de donación de órganos es complejo y demanda de un conjunto de recursos muy especializados e interconectados que operan con altas restricciones de tiempos. El presente trabajo analiza desde las perspectivas organizativas y sociales el Sistema Nacional de Trasplantes. Por medio de una metodología cualitativa basada en el desarrollo de un análisis Delphi, se presenta y describe el complejo sistema de donación de órganos, a continuación se analizan los factores de éxito del sistema de gestión. Se muestra como los mecanismos de coordinación intrahospitalarios, que promueven los hospitales, así como los extra hospitalarios que facilita la Organización Nacional de Trasplantes, posibilitan la colaboración y el consenso que se requieren para conseguir los resultados exitosos que hacen del modelo español un referente mundial. Los resultados apuntan que se dan un conjunto de elementos de gestión que constituyen factores críticos de éxito en el sistema, como las competencias de coordinación que desarrollan por un lado la Organización de Trasplantes y por otro los coordinadores hospitalarios en materia de donación de órganos, factores como la formación a los especialistas, la sensibilización hacia la importancia de la donación, la gestión y el control de la información y la dualidad en el perfil de los coordinadores constituyen los pilares básicos de este sistema excelente.

  11. Algunas reflexiones éticas sobre los trasplantes de órganos sólidos

    Directory of Open Access Journals (Sweden)

    P. Patricio Burdiles, Dr.

    2010-03-01

    Full Text Available Durante el año 2009 se evidenció una disminución en el número de donaciones de órganos en Chile, aún cuando las listas de espera de pacientes que requieren un trasplante de órganos sólidos han seguido aumentando. Estas materias contribuyen a generar gran preocupación en los pacientes, familiares y en los equipos de salud involucrados. La consolidación de los trasplantes como recursos terapéuticos probados y validados por varias décadas de experimentación y experiencias clínicas, trae como consecuencia que se anhele y promueva, desde distintos estamentos médicos, científicos y sociales, aumentar la tasa de donaciones. Sin embargo por ser de suyo un tema relativamente nuevo en nuestra sociedad, que involucra aspectos morales, espirituales y afectivos, es esperable que resulten en cuestionamientos y tensiones éticas. Esto mismo hace necesario que se genere, facilite y profundice en los actores sociales, un debate sobre esta materia, que toca en lo más profundo, nuestras concepciones respecto a la vida, la dignidad e integridad corporal y en torno a la muerte y a sus definiciones. La familiarización con estos temas y la comprensión de los principios y valores que fundamentan los trasplantes, contribuirá a reducir la paradójica realidad del discurso mayoritario a favor de la donación de órganos y el escaso número real de donaciones efectuadas en los últimos años.

  12. Elección de fluidos en el periodo perioperatorio del trasplante renal

    OpenAIRE

    Alejandro Gonzalez-Castro; María Ortiz-Lasa; Yhivian Peñasco; Camilo González; Carmen Blanco; Juan Carlos Rodriguez-Borregan

    2017-01-01

    El suero salino normal (SSN) ha sido clásicamente el fluido de resucitación elegido en el periodo perioperatorio del trasplante renal frente a aquellas soluciones balanceadas con potasio. Sin embargo, los problemas derivados de la hipercloremia desencadenada por la infusión de SSN han llevado a la realización de estudios que comparaban esta solución con los fluidos equilibrados. Mediante la presente revisión narrativa se deduce que el uso de cristaloides balanceados con contenido de potasio e...

  13. Evaluación de desenlace: trasplante meniscal versus segunda meniscectomía

    OpenAIRE

    Manrique Otero, Diana Marcela; Moncaleano Ruiz, Viviana; Peña Cruz, Nancy Tatiana; Ramírez Trujillo, Paula; Romero Mora, Jaime Alberto

    2016-01-01

    Introducción: El incremento de pacientes sintomáticos de rodilla y la osteoartrosis en jóvenes con limitadas posibilidades terapéuticas después de una meniscectomía, genera la búsqueda de alternativas terapéuticas. A pesar que es poco utilizado en Colombia, el trasplante meniscal es una propuesta para el manejo sintomático. Según cifras norteamericanas, se practican entre 700.000 a 1.500.000 artroscopias de rodilla anualmente, el 50% termina en meniscectomía y de este un 40% persiste...

  14. Recurrence of ANCA-associated vasculitis in a patient with kidney trasplant

    Directory of Open Access Journals (Sweden)

    Pedro García Cosmes

    2016-03-01

    Full Text Available Renal disease secondary to vasculitis associated with anti-neutrophil cytoplasmic antibodies (ANCA can lead to chronic renal disease requiring renal replacement therapy. In these patients, kidney transplantation offers excellent long-term rates of allograft and patient survival; consequently, they can be trasplanted when the clinical disease activity has remitted. However, the risk of disease relapses in the renal allograft remains, although at lower rates due to modern immunosuppressive regimes. We describe the case of a male patient with extracapillary glomerulonephritis type III C-ANCA (+ who developed a recurrence in the renal allograft 8 years after transplantation. Intensive immunosupression with plasmapheresis controlled the disease.

  15. Selección de donantes y receptores en trasplante pulmonar: procedimientos generales

    Directory of Open Access Journals (Sweden)

    C. María Teresa Parada, Dra.

    2010-03-01

    Full Text Available El trasplante pulmonar es una alternativa terapéutica que se ha validado en lo últimos 30 años para aquellos pacientes portadores de una enfermedad pulmonar terminal. Las características propias del trasplante pulmonar dadas por su conexión al ambiente a través de la vía aérea y la isquemia presente en las suturas durante el primer mes han constituído por mucho tiempo la principal causa de morbilidad y mortalidad de los pacientes trasplantados pulmonares. Los nuevos medicamentos inmunosupresores, la mejoría de las soluciones de preservación y de las técnicas quirúrgicas han disminuido las complicaciones y mortalidad precoz, siendo el gran desafío aún, el rechazo crónico conocido como el Sindrome de Bronquiolitis Obliterante (SBO. La selección del receptor de acuerdo a la patología de base se ha modificado, promoviendo la derivación precoz en patologías como la fibrosis pulmonar y la fibrosis quística disminuyendo así la mortalidad en la lista de espera.

  16. Seroprevalencia de citomegalovirus en donantes de órganos y receptores de trasplante renal, Colombia, 2010-2014

    Directory of Open Access Journals (Sweden)

    Yazmín Rocío Arias-Murillo

    2016-08-01

    Conclusiones. Los resultados del presente estudio evidenciaron que las tasas de infección por citomegalovirus fueron altas y que la categorización del riesgo de los receptores de trasplante señala la necesidad de que los equipos médicos tratantes tomen medidas para minimizar los riesgos.

  17. Evolución de la inmunología e influencia en los trasplantes de órganos

    OpenAIRE

    Carretero Vegas, Luz María

    2012-01-01

    Se profundiza en el conocimiento sobre los mecanismos del sistema inmunológico y la evolución histórica de los mismos para conocer la influencia de la inmunología en los trasplantes y las medidas preventivas más adecuadas para evitar las complicaciones

  18. [Occupational Hearing Loss (BK-No. 2301) - A Retrospective Analysis of 100 Consecutive Cases].

    Science.gov (United States)

    Reiter, R; Brosch, S

    2016-10-01

    Introduction: In order for a diagnosis of Occupational Hearing Loss (BK-no. 2301) to be made certain criteria must be fulfilled to establish that the hearing loss is occupational in origin. This work compares 2 groups, those who fulfil the criteria (BKE) and those who do not (BKNE). Methods: A 100 consecutive reports ("Lärmgutachten BK-no. 2301") written by the authors were examined retrospectively. These recorded audiometric examination, an analysis of any tinnitus and noise exposure plus use of hearing protection. Pre- and post-noise exposure status together with an expert assessment of work limitations was made to produce a 7 point score. Results: 67% of the group fulfilled the conditions for occupational hearing loss (9% were entitled to compensation). In the BKE group 82% showed typical audiometric signs of noise damage with 75% of them fulfilling at least 6 criteria of occupational disease no. 2301. Tinnitus typical for noise exposure was found in 26%. Discussion: A 7 point score could be useful in the future as a method of helping distinguish hearing loss and tinnitus from occupational as opposed to other causes. © Georg Thieme Verlag KG Stuttgart · New York.

  19. Evolución del peso en pacientes durante el primer año del trasplante renal

    Directory of Open Access Journals (Sweden)

    Pilar López Garrigós

    Full Text Available Durante el primer año de trasplante renal es frecuente una ganancia de peso, debido, entre otros a la dieta, sedentarismo, e inmunosupresores, pudiendo aumentar los factores de riesgo del síndrome metabólico. Objetivos: Conocer la evolución del peso de nuestros pacientes durante el primer año del trasplante, y analizar la influencia con las distintas variables asociadas al paciente, sexo, edad, IMC, tratamiento inmunosupresor entre otras. Determinar la asociación entre la ganancia de peso y la aparición de factores de riesgo, como hipertensión arterial, diabetes y dislipidemia. Material y Método: Estudio retrospectivo y analítico. Se estudiaron 100 pacientes con un año de evolución en el trasplante, del Hospital Virgen de la Salud de Toledo. Sé analizaron datos antropométricos, demográficos y clínicos. La ganancia de peso se distribuyo según a las variables del estudio. Resultados: La ganancia de peso al año del trasplante afecto a 93 pacientes, con una media de 7,21 kg ±5,87. El 44,1% aumento su peso por encima de la media, y el 23,7% gano > 10 kg. La prevalencia de sobrepeso y obesidad antes del trasplante fue 57% y 76% después del trasplante. Los hombres ganaron ligeramente más peso que las mujeres. Los más jóvenes < 49 años ganaron más peso, media 9,3 kg. Mayor incidencia de diabetes, hipertensión y dislipemias en los pacientes con sobrepeso y obesidad. Conclusiones: La mayoría de los pacientes aumentaron significativamente de peso al año del trasplante, asociándose con varios de los factores del síndrome metabólico. - Importante la educación, por parte de la enfermería, del paciente para fomentar hábitos de vida saludable.

  20. Perbedaan Pemahaman Guru BK Tentang Konseling Kelompok antara Alumni Unnes dan Non-Unnes

    Directory of Open Access Journals (Sweden)

    Desta Rizky Budiarti

    2014-10-01

    Full Text Available Tujuan penelitian ini untuk mengetahui gambaran pemahaman guru BK alumni Unnes dan guru BK alumni non-Unnes tentang konseling kelompok, dan perbedaan pemahaman diantara keduanya. Jenis penelitian adalah penelitian survey komparatif. Populasi penelitian ini yaitu guru BK di SMP Negeri se-Kota Semarang. Teknik sampling yang digunakan adalah Cluster Proportional Random Sampling. Metode pengumpulan data menggunakan tes tentang pemahaman konseling kelompok. Analisis datanya menggunakan analisis kuantitatif yang mencakup deskriptif prosentase dan uji beda t-test polled varian. Hasil penelitian menunjukkan bahwa ada perbedaan yang signifikan, dimana pemahaman guru BK alumni Unnes tentang konseling kelompok berada pada kategori sangat tinggi dengan persentase 84,26% dibandingkan dengan guru BK alumni non-Unnes yang memiliki persentase 63,9% berada pada kategori sedang. Simpulan dari penelitian ini, pemahaman guru BK tentang konseling kelompok antara alumni Unnes lebih tinggi daripada guru BK alumni non-Unnes. The purpose of this study to describe the understanding of BK teacher Unnes graduate and BK teacher non - Unnes graduates about group counseling, and understanding the differences between them . This type of research is a comparative survey research. The population of this study are in Junior High School teacher BK as the city of Semarang. The sampling technique used is proportional cluster random sampling. Methods of data collection using test on understanding group counseling. Analysis of the data using descriptive quantitative analysis that includes the percentage and t - test different test variants polled. The results showed that there were significant differences, where the understanding of BK teacher Unnes graduate  abaout  the counseling group in the category with a very high percentage of 84.26 % compared to a BK teacher non - Unnes graduates who have a percentage only 63.9 % are in the medium category. The conclusions of this study

  1. Incidence and Factors Associated with De Novo DSA After BK Viremia in Renal Transplant Recipients.

    Science.gov (United States)

    Patel, Samir J; Kuten, Samantha A; Knight, Richard J; Graviss, Edward A; Nguyen, Duc; Gaber, A Osama

    2016-01-01

    BK polyomavirus infection and de novo donor-human leukocyte antigen (HLA) specific antibodies (dnDSA) are two well-known and distinct complications occurring after kidney transplantation. Recent literature suggests an association between the two events. This study aims to examine the relationship between BK viremia (BKV) and dnDSA and to identify potential risk factors for dnDSA following BKV in kidney transplant recipients. A retrospective review of 1019 recipients from Houston Methodist Hospital was conducted. All patients underwent routine screening for BKV and dnDSA. Median follow-up was 44 months. BKV was detected in 186 (18%) patients at a median of 107 (82-205) days post-transplant. dnDSA occurred in 283 (28%) patients at a median of 272 (62-575) days post-transplant. Of the 69 dnDSA-positive/BKV-positive patients, dnDSA detection occurred after BKV onset in 46 patients. Thus, 46 (28%) previously DSA-negative patients later became dnDSA-positive following BKV, not significantly different from the rate seen in BKV-negative patients (26%; p=0.5). Median time to DSA detection following BKV onset was 232 days (interquartile range, 119-460) post-BKV detection. Multivariate analysis revealed a greater number of HLA mismatches and viral clearance as risk factors for development of dnDSA following BKV, whereas delayed graft function was associated with a lower risk of dnDSA. In conclusion, despite being considered a result of over-immunosuppression, BKV can still be followed by dnDSA in a substantial proportion of patients. Monitoring for dnDSA in patients being managed for BKV may be warranted. Copyright© 2017 by the Terasaki Research Institute.

  2. Universal features of JIMWLK and BK evolution at small x

    CERN Document Server

    Rummukainen, K; Rummukainen, Kari; Weigert, Heribert

    2004-01-01

    In this paper we present the results of numerical studies of the JIMWLK and BK equations with a particular emphasis on the universal scaling properties and phase space structure involved. The results are valid for near zero impact parameter in DIS. We demonstrate IR safety due to the occurrence of a rapidity dependent saturation scale Q_s(\\tau). Within the set of initial conditions chosen both JIMWLK and BK equations show remarkable agreement. We point out the crucial importance of running coupling corrections to obtain consistency in the UV. Despite the scale breaking induced by the running coupling we find that evolution drives correlators towards an asymptotic form with near scaling properties. We discuss asymptotic features of the evolution, such as the \\tau- and A-dependence of Q_s away from the initial condition.

  3. Evaluation of fluoroquinolones for the prevention of BK viremia after renal transplantation.

    Science.gov (United States)

    Gabardi, Steven; Waikar, Sushrut S; Martin, Spencer; Roberts, Keri; Chen, Jie; Borgi, Lea; Sheashaa, Hussein; Dyer, Christine; Malek, Sayeed K; Tullius, Stefan G; Vadivel, Nidyanandh; Grafals, Monica; Abdi, Reza; Najafian, Nader; Milford, Edgar; Chandraker, Anil

    2010-07-01

    Nearly 30% of renal transplant recipients develops BK viremia, a prerequisite for BK nephropathy. Case reports have evaluated treatment options for BK virus, but no controlled studies have assessed prophylactic therapies. Fluoroquinolone antibiotics were studied for prevention of BK viremia after renal transplantation. This retrospective analysis evaluated adult renal transplant recipients with at least one BK viral load (blood) between 90 and 400 days after transplantation. Six to 12 months of co-trimoxazole was used for Pneumocystis prophylaxis. In sulfa-allergic/-intolerant patients, 6 to 12 months of atovaquone with 1 month of a fluoroquinolone was used. Fluoroquinolones can inhibit BK DNA topoisomerase. The two groups studied were those that received 30 days of levofloxacin or ciprofloxacin after transplantation and those that did not. The primary endpoint was BK viremia rates at 1 year. Of note, of the 160 patients not receiving fluoroquinolone prophylaxis, 40 received a fluoroquinolone for treatment of a bacterial infection within 3 months after transplantation. Subgroup analysis evaluating these 40 patients against the 120 who had no exposure to fluoroquinolones was completed. A 1-month fluoroquinolone course after transplantation was associated with significantly lower rates of BK viremia at 1 year compared with those with no fluoroquinolone. In the subgroup analysis, exposure to fluoroquinolone for treatment of bacterial infections within 3 months after transplantation was associated with significantly lower 1-year rates of BK viremia. This analysis demonstrates that fluoroquinolones are effective at preventing BK viremia after renal transplantation.

  4. Trasplante cardíaco en pacientes con enfermedad de Chagas. Experiencia de un único centro

    Directory of Open Access Journals (Sweden)

    Alfredo Inácio Fiorelli

    2011-07-01

    Conclusiones: El trasplante cardíaco es el único tratamiento actual eficaz de tratamiento de la enfermedad de Chagas en fase terminal. La reactivación de la enfermedad es un problema real que es fácilmente revertido con la introducción de la terapéutica farmacológica específica, restaurando los padrones histológicos del miocardio sin dejar secuelas. La inmunosupresión, en especial los corticoides, predisponen al desarrollo de neoplasias y a la reactivación de la enfermedad, exigiendo una atención especial su interrupción o reducción precoz.

  5. Pemahaman Guru BK Tentang Pelaksanaan Layanan Peminatan pada Kurikulum 2013

    Directory of Open Access Journals (Sweden)

    Finda Marsetyana

    2015-04-01

    Full Text Available Penelitian ini dilakukan berdasar fenomena yaitu belum adanya kesiapan tentang program peminatan di SMK Negeri se-Kota Semarang. Tujuan penelitian secara umum untuk mengetahui pemahaman guru BK tentang pelaksanaan layanan peminatan pada kurikulum 2013 di SMK Negeri se-Kota Semarang. Medote pengumpulan data yaitu angket tertutup yang diberikan kepada 44 orang guru BK SMK Negeri se Kota Semarang. Analisis data menggunakan analisis deskriptif persentase. Hasil penelitian dari Pemahaman Guru BK Tentang Pelaksanaan Layanan Peminatan Pada Kurikulum 2013 Di SMK Negeri Se-Kota Semarang termasuk dalam kategori tinggi (71.59% dengan perincian indikator variabel yaitu pemahaman guru BK tentang kurikulum 2013 persentase sebesar 75.04% (tinggi, konsep dan strategi pelayanan bimbingan dan konseling pada kurikulum 2013 sebesar 69.31% (sedang, lingkup layanan peminatan sebesar 70.57% (tinggi, dan pelaksanaan layanan peminatan 72.22% (tinggi. Simpulan penelitian ini yakni guru BK SMK Negeri se-Kota Semarang telah mempunyai pemahaman tentang pelaksanaan layanan peminatan pada kurikulum 2013 dengan kriteria tinggi. This research was conducted based on the phenomenon that is there was no readiness about the students’ interest program vocational high schools in around Semarang.The general objective of this research was to know the counseling’s teachers deals with the implementation of students’ interest service towards the 2013 curriculum vocational high schools in around Semarang. Method of data collection is used closed questionnaire and it was given to 44 students vocational high schools in around Semarang in which the data obtained was analysed trough descriptive percentages. The research finding from counseling’s teachers about the implementation of students’ interest in the 2013 curriculum vocational high schools in around Semarang are in a high category (71.59%, with the details of the indicator variable are as follows; the understanding of

  6. Reactivation of BK polyomavirus in patients with multiple sclerosis receiving natalizumab therapy.

    LENUS (Irish Health Repository)

    Lonergan, Roisin M

    2012-02-01

    Natalizumab therapy in multiple sclerosis has been associated with JC polyomavirus-induced progressive multifocal leucoencephalopathy. We hypothesized that natalizumab may also lead to reactivation of BK, a related human polyomavirus capable of causing morbidity in immunosuppressed groups. Patients with relapsing remitting multiple sclerosis treated with natalizumab were prospectively monitored for reactivation of BK virus in blood and urine samples, and for evidence of associated renal dysfunction. In this cohort, JC and BK DNA in blood and urine; cytomegalovirus (CMV) DNA in blood and urine; CD4 and CD8 T-lymphocyte counts and ratios in peripheral blood; and renal function were monitored at regular intervals. BK subtyping and noncoding control region sequencing was performed on samples demonstrating reactivation. Prior to commencement of natalizumab therapy, 3 of 36 patients with multiple sclerosis (8.3%) had BK viruria and BK reactivation occurred in 12 of 54 patients (22.2%). BK viruria was transient in 7, continuous in 2 patients, and persistent viruria was associated with transient viremia. Concomitant JC and CMV viral loads were undetectable. CD4:CD8 ratios fluctuated, but absolute CD4 counts did not fall below normal limits. In four of seven patients with BK virus reactivation, transient reductions in CD4 counts were observed at onset of BK viruria: these resolved in three of four patients on resuppression of BK replication. No renal dysfunction was observed in the cohort. BK virus reactivation can occur during natalizumab therapy; however, the significance in the absence of renal dysfunction is unclear. We propose regular monitoring for BK reactivation or at least for evidence of renal dysfunction in patients receiving natalizumab.

  7. Donación de órganos para trasplante: una oportunidad para la equidad en chile

    Directory of Open Access Journals (Sweden)

    C. Marcelo Muñoz, Dr.

    2010-03-01

    Full Text Available La donación efectiva de órganos en Chile se encuentra en un preocupante estancamiento. La tasa de donantes efectivos no sólo no se ha incrementado en el periodo 1998–2008, sino que por el contrario en los últimos años ha disminuido. Cualquier análisis en estas circunstancias debe interpelar a los principios o a priori que fundamentan el actual sistema de donación y trasplante. Un modelo de donación de órganos para trasplante y todas aquellas etapas que permiten el éxito de esta técnica deben fundamentarse en el respeto a un principio ético central que es la equidad y en el resguardo con celo, la eficiencia, transparencia y credibilidad del mismo.

  8. Single-channel kinetics of BK (Slo1 channels

    Directory of Open Access Journals (Sweden)

    Yanyan eGeng

    2015-01-01

    Full Text Available Single-channel kinetics has proven a powerful tool to reveal information about the gating mechanisms that control the opening and closing of ion channels. This introductory review focuses on the gating of large conductance Ca2+- and voltage-activated K+ (BK or Slo1 channels at the single-channel level. It starts with single-channel current records and progresses to presentation and analysis of single-channel data and the development of gating mechanisms in terms of discrete state Markov (DSM models. The DSM models are formulated in terms of the tetrameric modular structure of BK channels, consisting of a central transmembrane pore-gate domain (PGD attached to four surrounding transmembrane voltage sensing domains (VSD and a large intracellular cytosolic domain (CTD, also referred to as the gating ring. The modular structure and data analysis shows that the Ca2+ and voltage dependent gating considered separately can each be approximated by 10-state two-tiered models with 5 closed states on the upper tier and 5 open states on the lower tier. The modular structure and joint Ca2+ and voltage dependent gating are consistent with a 50 state two-tiered model with 25 closed states on the upper tier and 25 open states on the lower tier. Adding an additional tier of brief closed (flicker states to the 10-state or 50-state models improved the description of the gating. For fixed experimental conditions a channel would gate in only a subset of the potential number of states. The detected number of states and the correlations between adjacent interval durations are consistent with the tiered models. The examined models can account for the single-channel kinetics and the bursting behavior of gating. Ca2+ and voltage activate BK channels by predominantly increasing the effective opening rate of the channel with a smaller decrease in the effective closing rate. Ca2+ and depolarization thus activate by mainly destabilizing the closed states.

  9. Conversion from tacrolimus-mycophenolate mofetil to tacrolimus-mTOR immunosuppression after kidney-pancreas transplantation reduces the incidence of both BK and CMV viremia.

    Science.gov (United States)

    Knight, Richard J; Graviss, Edward A; Nguyen, Duc T; Kuten, Samantha A; Patel, Samir J; Gaber, Lillian; Gaber, A Osama

    2018-04-19

    We sought to determine whether conversion from tacrolimus/mycophenolate mofetil (TAC-MMF) into tacrolimus/mTOR inhibitor (TAC-mTOR) immunosuppression would reduce the incidences of BK and CMV viremia after kidney/pancreas (KP) transplantation. In this single-center review, the TAC-mTOR cohort (n = 39) was converted at 1 month post-transplant to an mTOR inhibitor and reduced-dose tacrolimus. Outcomes were compared to a cohort of KP recipients (n = 40) maintained on TAC-MMF. At 3 years post-transplant, KP survivals and incidences of kidney/pancreas rejection were equivalent between mTOR and MMF-treated cohorts. (P = ns). BK viremia-free survival was better for the mTOR vs MMF-treated group (P = .004). In multivariate analysis, MMF vs mTOR immunosuppression was an independent risk factor for BK viremia (hazard ratio 12.27, P = .02). Similarly, mTOR-treated recipients displayed better CMV infection-free survival compared to the MMF-treated cohort (P = .01). MMF vs mTOR immunosuppression (hazard ratio 18.77, P = .001) and older recipient age (hazard ratio 1.13 per year, P = .006) were independent risk factors for CMV viremia. Mean estimated GFR and HgbA1c levels were equivalent between groups at 1, 2, and 3 years post-transplantation. Conversion from TAC/MMF into TAC/mTOR immunosuppression after KP transplantation reduced the incidences of BK and CMV viremia with an equivalent risk of acute rejection and similar renal/pancreas function. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  10. A Precise determination of B(K) in quenched QCD

    CERN Document Server

    Dimopoulos, P.; Palombi, F.; Pena, C.; Sint, S.; Vladikas, A.

    2006-01-01

    The $B_K$ parameter is computed in quenched lattice QCD with Wilson twisted mass fermions. Two variants of tmQCD are used; in both of them the relevant $\\Delta S = 2$ four-fermion operator is renormalised multiplicatively. The renormalisation adopted is non-perturbative, with a Schroedinger functional renormalisation condition. Renormalisation group running is also non-perturbative, up to very high energy scales. In one of the two tmQCD frameworks the computations have been performed at the physical $K$-meson mass, thus eliminating the need of mass extrapolations. Simulations have been performed at several lattice spacings and the continuum limit was reached by combining results from both tmQCD regularisations. Finite volume effects have been partially checked and turned out to be small. Exploratory studies have also been performed with non-degenerate valence flavours. The final result for the RGI bag parameter, with all sources of uncertainty (except quenching) under control, is $\\hat B_K =0.789 \\pm 0.046$.

  11. Trasplante simultáneo de páncreas y riñón: reporte del primer caso realizado en el Perú

    Directory of Open Access Journals (Sweden)

    José De Vinatea

    2010-04-01

    Full Text Available El trasplante simultáneo de páncreas y riñón es la opción terapéutica ideal para pacientes con diabetes mellitus tipo 1 con insuficiencia renal crónica. Permite recuperar la insulino-independencia y evita la necesidad de diálisis, mejorando notablemente la calidad de vida. Así mismo, la restitución de la euglicemia logra que las complicaciones tardías de la diabetes no progresen e incluso regresionen, prolongando la supervivencia global de estos pacientes. Estos beneficios superan a los riesgos de la cirugía y de la inmunosupresión inherentes al trasplante. A nivel mundial, desde 1966, en que se llevó a cabo el primer trasplante de páncreas en Minnesota (Estados Unidos, se han registrado más de 20 000 trasplantes de páncreas, con resultados cada vez mejores gracias al refinamiento en las técnicas quirúrgicas y a los nuevos esquemas de inmunosupresión. En el presente caso clínico, se reporta y describe la realización del primer trasplante simultáneo de páncreas y riñón en el Perú.

  12. Differential contribution of Ca2+ sources to day and night BK current activation in the circadian clock.

    Science.gov (United States)

    Whitt, Joshua P; McNally, Beth A; Meredith, Andrea L

    2018-02-05

    Large conductance K + (BK) channels are expressed widely in neurons, where their activation is regulated by membrane depolarization and intracellular Ca 2+ (Ca 2+ i ). To enable this regulation, BK channels functionally couple to both voltage-gated Ca 2+ channels (VGCCs) and channels mediating Ca 2+ release from intracellular stores. However, the relationship between BK channels and their specific Ca 2+ source for particular patterns of excitability is not well understood. In neurons within the suprachiasmatic nucleus (SCN)-the brain's circadian clock-BK current, VGCC current, and Ca 2+ i are diurnally regulated, but paradoxically, BK current is greatest at night when VGCC current and Ca 2+ i are reduced. Here, to determine whether diurnal regulation of Ca 2+ is relevant for BK channel activation, we combine pharmacology with day and night patch-clamp recordings in acute slices of SCN. We find that activation of BK current depends primarily on three types of channels but that the relative contribution changes between day and night. BK current can be abrogated with nimodipine during the day but not at night, establishing that L-type Ca 2+ channels (LTCCs) are the primary daytime Ca 2+ source for BK activation. In contrast, dantrolene causes a significant decrease in BK current at night, suggesting that nighttime BK activation is driven by ryanodine receptor (RyR)-mediated Ca 2+ i release. The N- and P/Q-type Ca 2+ channel blocker ω-conotoxin MVIIC causes a smaller reduction of BK current that does not differ between day and night. Finally, inhibition of LTCCs, but not RyRs, eliminates BK inactivation, but the BK β2 subunit was not required for activation of BK current by LTCCs. These data reveal a dynamic coupling strategy between BK channels and their Ca 2+ sources in the SCN, contributing to diurnal regulation of SCN excitability. © 2018 Whitt et al.

  13. Sarcoma de Kaposi bucal en pacientes con trasplante de riñón

    Directory of Open Access Journals (Sweden)

    Gladys Aída Estrada Pereira

    2015-02-01

    Full Text Available Se realizó un estudio descriptivo y transversal de 25 pacientes con trasplante renal y sarcoma de Kaposi bucal, atendidos en la consulta estomatológica del Policlínico de Especialidades del Hospital Clinicoquirúrgico Docente "Saturnino Lora Torres" de Santiago de Cuba, desde marzo de 2008 hasta igual mes de 2013, para describir los resultados clínicos e histopatológicos. Entre los hallazgos principales predominaron el sexo masculino, el grupo etario de 40-49 años y los afectados de piel negra. Por otra parte, como alteraciones hísticas sobresalieron los espacios vasculares atípicos, los cuerpos eosinófilos y la extravasación de hematíes; asimismo, la mayoría de las lesiones correspondieron al paladar duro y a la encía. La biopsia resultó ser un medio de diagnóstico valioso para confirmar esta enfermedad

  14. CALIDAD DE VIDA RELACIONADA CON LA SALUD INFANTIL Y EL TRASPLANTE DE ÓRGANOS: UNA REVISIÓN DE LITERATURA

    Directory of Open Access Journals (Sweden)

    Elisa Kern de Castro

    2005-06-01

    Full Text Available En el presente artículo los autores revisan algunas cuestiones teóricas y estudios empíricos sobre la calidad de vida relacionada con la salud infantil, en especial en niños con enfermedades crónicas que necesitan trasplantes de órganos. Pocas investigaciones han sido realizadas sobre el tema hasta el momento. Sus resultados han mostrado que el trasplante mejora el bienestar físico pero no hay conclusiones definitivas sobre su relación con el bienestar psicológico. Se discute la necesidad de evaluar los efectos del trasplante de órganos pediátrico en cada fase evolutiva, en distintos órganos y en diversas culturas.

  15. En la Cuyuntura entre la Antropología y el Trasplante de Órganos Humanos: Tendencias, Conceptos y Agendas.

    Directory of Open Access Journals (Sweden)

    Alejandro Castillejo Cuéllar.

    2008-01-01

    Full Text Available Este texto es un resumen de los debates en torno a la tecnología de trasplante de órganos humanos desde la perspectiva de las ciencias sociales. El artículo se divide en cuatro secciones: por un lado, presenta algunas de las principales corrientes relativas a la ética de los trasplantes de órganos humanos. En segundo lugar, que habita en el encuentro de profesionales y la antropología de la práctica médica. En tercer lugar, el artículo discute los principales problemas con el trasplante de lo que se refiere a la traffcking de órganos humanos. Por último, se alude a los programas de investigación pertinentes posible en América Latina, y particularmente en Colombia.

  16. La perspectiva de los nefrólogos acerca de la donación y el trasplante renal en la Argentina

    Directory of Open Access Journals (Sweden)

    Javier Roberti

    2014-10-01

    Full Text Available El objetivo de este trabajo cualitativo fue explorar y describir la perspectiva de los nefrólogos acerca de la situación de la donación y el trasplante en la Argentina. Participaron 22 jefes de equipo de trasplante y/o profesionales a cargo de la toma de decisiones sobre la aceptación de injertos, donantes y candidatos a trasplante, de una muestra intencional. Se realizaron entrevistas en profundidad, semi-estructuradas, que se grabaron, transcribieron, analizaron y codificaron temáticamente. Más de la mitad de los participantes coincidió en la necesidad de promover la donación de órganos; casi la mitad dijo que los centros de diálisis no enviaban todos los pacientes que deberían a realizar estudios para trasplante. La mitad de los participantes confirmó que la información brindada por el equipo de procuración no era confiable. Se identificó la falta de adherencia de los pacientes al tratamiento como un problema serio, principalmente a causa de la situación socio-económica. 5/22 participantes expresaron que la cantidad de trasplantes no aumentaba por bajas tasas de donación, entrenamiento deficiente o falta de información de los nefrólogos. Los participantes percibieron una necesidad de información, entrenamiento y compromiso de los profesionales de la salud en la identificación y derivación de posibles donantes y receptores y señalaron que los problemas socioeconómicos afectan al proceso en diferentes etapas. Estos temas deberían ser tenidos en cuenta para mejorar los resultados del trasplante en un contexto de transparencia y equidad.

  17. Phase 1b randomized trial and follow-up study in Uganda of the blood-stage malaria vaccine candidate BK-SE36.

    Science.gov (United States)

    Palacpac, Nirianne Marie Q; Ntege, Edward; Yeka, Adoke; Balikagala, Betty; Suzuki, Nahoko; Shirai, Hiroki; Yagi, Masanori; Ito, Kazuya; Fukushima, Wakaba; Hirota, Yoshio; Nsereko, Christopher; Okada, Takuya; Kanoi, Bernard N; Tetsutani, Kohhei; Arisue, Nobuko; Itagaki, Sawako; Tougan, Takahiro; Ishii, Ken J; Ueda, Shigeharu; Egwang, Thomas G; Horii, Toshihiro

    2013-01-01

    Up to now a malaria vaccine remains elusive. The Plasmodium falciparum serine repeat antigen-5 formulated with aluminum hydroxyl gel (BK-SE36) is a blood-stage malaria vaccine candidate that has undergone phase 1a trial in malaria-naive Japanese adults. We have now assessed the safety and immunogenicity of BK-SE36 in a malaria endemic area in Northern Uganda. We performed a two-stage, randomized, single-blinded, placebo-controlled phase 1b trial (Current Controlled trials ISRCTN71619711). A computer-generated sequence randomized healthy subjects for 2 subcutaneous injections at 21-day intervals in Stage1 (21-40 year-olds) to 1-mL BK-SE36 (BKSE1.0) (n = 36) or saline (n = 20) and in Stage2 (6-20 year-olds) to BKSE1.0 (n = 33), 0.5-mL BK-SE36 (BKSE0.5) (n = 33), or saline (n = 18). Subjects and laboratory personnel were blinded. Safety and antibody responses 21-days post-second vaccination (Day42) were assessed. Post-trial, to compare the risk of malaria episodes 130-365 days post-second vaccination, Stage2 subjects were age-matched to 50 control individuals. Nearly all subjects who received BK-SE36 had induration (Stage1, n = 33, 92%; Stage2, n = 63, 96%) as a local adverse event. No serious adverse event related to BK-SE36 was reported. Pre-existing anti-SE36 antibody titers negatively correlated with vaccination-induced antibody response. At Day42, change in antibody titers was significant for seronegative adults (1.95-fold higher than baseline [95% CI, 1.56-2.43], p = 0.004) and 6-10 year-olds (5.71-fold [95% CI, 2.38-13.72], p = 0.002) vaccinated with BKSE1.0. Immunogenicity response to BKSE0.5 was low and not significant (1.55-fold [95% CI, 1.24-1.94], p = 0.75). In the ancillary analysis, cumulative incidence of first malaria episodes with ≥5000 parasites/µL was 7 cases/33 subjects in BKSE1.0 and 10 cases/33 subjects in BKSE0.5 vs. 29 cases/66 subjects in the control group. Risk ratio for BKSE1.0 was 0.48 (95% CI, 0

  18. Trasplantation of larynx and trachea: both a present and future option Trasplantes de la laringe y tráquea, una opción para el presente y el futuro

    Directory of Open Access Journals (Sweden)

    Francisco Martínez

    2004-01-01

    Full Text Available

    Lost functions of the larynx and the trachea may be restored by different means, but none of them is physiologic and patients are left with functional difficulties and self-esteem disorders that alter the quality of their lives.  Transplantation of these organs is an useful alternative but certain basic requirements concerning revascularization, reinnervation and immunosupression should be fulfilled.  The former has been achieved through the knowledge of vascular territories and the advances in surgical techniques.  Difficulties for reinnervation have no yet been thoroughly overcome but tone can be achieved in the vocal folds and functionality may be obtained by manipulation of their position.  Immunosupression is produced with cyclosporine which requirements were determined in experimental models.  The main reason for carrying out these and other transplantations is to improve the quality of life of persons who have suffered the loss of an organ.  A review is presented on important aspects that have to be taken into account when performing these transplantations.

    Las funciones perdidas de la laringe o la tráquea se pueden reemplazar de diferentes maneras; sin embargo, ninguna alternativa es fisiológica y los trasplantes quedan con dificultades funcionales y de autoestima que alteran la calidad de sus vidas,  El Trasplante de estos órganos es una alternativa útil, pero deben cumplirse ciertos requisitos básicos en cuanto a la revascularización, la reinervación y la imunosupresión.  El primero se logró con el conocimiento de los territorios vasculares y los avances en las técnicas quirúrgicas.  Las dificultades para la reinervación todavía no se han superado totalmente, pero se puede lograr tono en los pliegues vocales y obtener funcionalidad manipulando su posición.  la inmunosupresión se logra con ciclosporina cuyos requerimientos se definieron en modelos experimentales.  La principal razón para hacer estos y

  19. De Novo collapsing glomerulopathy in renal allograft in association with BK virus nephropathy in a child and stabilization of renal function by elimination of viremia

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    D N Gera

    2017-01-01

    Full Text Available Well-recognized association between HIV 1 infection and collapsing glomerulopathy (CG raises the possibility that intrarenal infection by other viruses may also contribute to the development of this lesion in native or post-transplant kidneys. There is evidence in literature about association of these lesions with cytomegalovirus, Epstein–Barr virus, hepatitis C virus, and parvovirus B19 infections. Here, we present a case report of post-transplant BK virus nephropathy in a male child who was found to have CG in subsequent biopsy 2 months later. His renal function and proteinuria were stabilized on elimination of viremia.

  20. Trasplante renal con HLA idéntico de donante vivo y cadavérico: experiencia de la Fundación Valle de Lili, Cali, Colombia

    OpenAIRE

    Caicedo, Luis Armando; Gómez-Vega, Juan Carlos; Duque, Mauricio Francisco; Serrano, Óscar Javier; Manzi, Eliana; Arrunátegui, Ana María; Posada, Juan Guillermo; Mesa, Liliana; Schweineberg, Johanna; Durán, Carlos Eduardo; Villegas, Jorge Iván; Dávalos, Diana María; Echeverri, Gabriel Jaime

    2016-01-01

    Introducción: En el trasplante renal con HLA idéntico los episodios de rechazo agudo son menores y tienen mejores tasas de supervivencia del injerto, comparado con los receptores con HLA no idéntico; a pesar de esto, persiste el dilema en cuanto al retiro o la disminución de la dosis de inmunosupresión. El objetivo de este trabajo es describir la experiencia de los trasplantes renales con HLA idéntico de donante vivo y cadavérico que se han realizado en la Fundación Valle del Lili desde 1995 ...

  1. Evaluación de una herramienta educativa en conocimiento de donación y trasplante de órganos y tejidos en Colombia

    OpenAIRE

    Gómez Bossa, Mauricio Andrés; Mijares Benavides, Cesar Augusto

    2014-01-01

    El trasplante de órganos es considerado uno de los avances más significativos de la medicina moderna y es un procedimiento cada vez más exitoso en términos de supervivencia de los pacientes, siendo actualmente la mejor opción de tratamiento para los pacientes con innumerables patologías. El proceso de donación es insuficiente para cubrir las necesidades de trasplante de la población, por lo tanto, se hace necesario el desarrollo de nuevas estrategias para fortalecer la experiencia y efectiv...

  2. Infeccion urinaria temprana en trasplante renal: Factores de riesgo y efecto en la sobrevida del injerto Early urinary tract infection in kidney transplantation: Risk factors and impact on graft sur-vival

    Directory of Open Access Journals (Sweden)

    Pablo A. Cepeda

    2005-10-01

    Full Text Available La infección urinariatemprana del injerto (IUTI, definida como infección urinaria sintomática en los primeros 3 meses del trasplante, su efecto sobre la sobrevida del injerto y los factores de riesgo han sido poco estudiados. Los objetivos del presente análisis fueron conocer factores de riesgo para IUTI, analizar agentes causantes e impacto en la sobrevida del injerto. En forma retrospectiva se analizaron pacientes que recibieron trasplante renal durante 1997-2000 en el Hospital Privado - Centro Médico de Córdoba. Se dividió en dos grupos de pacientes, según presencia (grupo IUTI o ausencia (grupo control de IUTI. Los factores de riesgo se analizaron con el modelo de riesgos proporcionales de Cox y la sobrevida del injerto con el método de Kaplan-Meier. Recibieron trasplante renal 226 pacientes consecutivos. La IUTI se presentó en 55 (24.3%. Factores de riesgo asociados con IUTI: antecedentes de maniobras urológicas invasivas (RR=4.34, IC 95% 1.42-13.21, diabetes mellitus (RR=3.79, IC 95% 1.42-10.14, infección por citomegalovirus (RR=2.9, IC 95% 1.02-8.24 y antecedente de trasplante previo (RR=2.83, IC 95% 1.08-7.45. El retardo en la función del injerto (RR=0.38, IC 95% 0.15-0.94 se asoció con menor incidencia de IUTI. Agentes más frecuentes: Klebsiella pneumoniae (36%, Pseudomonas aeruginosa (24% y Escherichia coli (9%. La sobrevida del injerto a los 2 años en el grupo IUTI (87.2% no fue diferente del control (81.2%, P = 0.32. En esta serie las maniobras urológicas invasivas fueron el principal factor de riesgo asociado a IUTI. No hubo disminución de la sobrevida del injerto asociada a IUTI. La alta prevalencia de uropatógenos no coli requiere mayor evaluación.The early urinary tract infection (EUTI in kidney transplant recipients is an infection develop during the first 3 months post transplant surgery. The effect of EUTI on graft survival and risk factors have been scarcely studied. Our objetives were the evaluation of

  3. Influencia de cuatro distancias de trasplante sobre el rendimiento agrícola del cultivar de arroz Amistad-82.

    Directory of Open Access Journals (Sweden)

    Alexander Calero Hurtado

    2012-04-01

    Full Text Available El trabajo consistió en comparar cuatro distancias de trasplante sobre el rendimiento agrícola del cultivar de arroz Amistad-82, durante la siembra de primavera de 2012, el trasplante se realizó de forma manual, sobre un suelo pardo sialítico carbonatado en la finca “La Rosita” perteneciente a la CCS “Heriberto Orellane”, en la provincia de Sancti Spíritus, se utilizó un diseño de bloques al azar, se tomaron posturas jóvenes de 18 días de germinadas, cuatro distancias de plantación 10x15, 15x15, 20x15 y 20x20 cm y una postura por sitio. Se evaluaron los indicadores productivos como el número de tallos, panículas por metro cuadrado, longitud de la panícula, granos por panículas, granos llenos y vanos por panículas, peso de 1000 granos, el rendimiento, así como las ganancias y los costos de producción. Los mejores resultados sobre el rendimiento agrícola del cultivar de arroz Amistad-82 lo alcanzaron las distancias de trasplante 10x15 y 20x15 cm, porque lograron rendimientos superiores a las distancias 15x15 y 20x20 cm, mayor cantidad de granos por panículas y mayores porcentajes de granos llenos por panículas y superaron también el peso en 1000 granos y las ganancias.

  4. Fenestración videolaparoscópica de un linfocele sintomático posterior a un trasplante renal

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    Miguel Ángel Martín González

    2003-09-01

    Full Text Available Este trabajo tiene el objetivo de describir un caso tratado por videolaparoscopia, de un linfocele sintomático posterior a trasplante renal. Se refiere a una mujer de 54 años con insuficiencia renal crónica terminal, secundaria a hipertensión arterial severa, la cual se le realizó en el 2001 un trasplante renal de donante cadáver. Presenta, 3 meses después, molestias en la zona del trasplante, sin síntomas urinarios por la presencia de un catéter ureteral utilizado como férula y en tiempo para ser retirado. Se diagnostica un linfocele sintomático. Posterior al chequeo preoperatorio se efectúa drenaje interno por vía videolaparoscópica sin accidentes ni complicaciones quirúrgicas inmediatas y el retirado del catéter ureteral y el alta a las 24 y 48 h de la operación, respectivamente. Después de 10 meses de evolución no se ha encontrado ninguna complicación. El abordaje videolaparoscópico del linfocele sintomático posterior al trasplante renal constituye la terapéutica de elecciónThis paper is aimed at describing a case of symptomatic lymphocele after kidney transplantation, which was treated with videolaparoscopy. It was a 54 years-old woman with terminal chronic renal failure secondary to several blood hypertension, who was transplanted a kidney from a dead donor in 2001. She presented with some discomfort in the transplant area, without urinary symptoms, owing to a urethral catheter used as rod and due to be withdrawn. The diagnosis was symptomatic lymphocele. After pre-operative checking, internal drainage by videolaparoscopy was performed, with no accidents or immediate surgical complications; the urethral catheter was withdrawn at 24 hours and the patient was discharged at 48 hours. Ten months later, no complication was found. The videolaparoscopic treatment of symptomatic lymphocele after kidney transplantation is the therapy of choice

  5. Bk and Cf chromatographic separation and 249Bk/248Cm and 249Cf/248Cm elemental ratios determination by inductively coupled plasma quadrupole mass spectrometry

    International Nuclear Information System (INIS)

    Gourgiotis, A.; Isnard, H.; Nonell, A.; Aubert, M.; Stadelmann, G.; Dupont, E.; AlMahamid, I.; Tiang, G.; Rao, L.; Lukens, W.; Cassette, P.; Panebianco, S.; Letourneau, A.; Chartier, F.

    2013-01-01

    The French Atomic Energy Commission has carried out several experiments for the study of minor-actinide transmutation processes in high intensity thermal neutron flux. In this context a Cm sample enriched in 248 Cm (97%) was irradiated in a thermal neutron flux at the High Flux Reactor (HFR) of the Laue-Langevin Institute (ILL). The precise and accurate determination of Cf isotope ratios and of 249 Bk/ 248 Cm and 249 Cf/ 248 Cm elemental ratios in the 248 Cm irradiated sample is crucial for the calculation of actinide neutron capture cross-sections.This work describes an analytical procedure for the separation and the isotope ratio measurement of Bk and Cf in the irradiated sample.The Bk and Cf separation is based on a lanthanides separation protocol previously developed by the laboratory. Well-defined retention times for Bk and Cf were obtained by coupling the Ionic Chromatography (IC) with an ICP-QMS. All conditions of element separation by IC and the different steps of the analytical protocol in order to obtain the isotopic and elemental ratios are presented. Relative uncertainties of Cf isotopic ratios range from 0.3% to 0.5% and the uncertainty of the 249 Bk/ 248 Cm and 249 Cf/ 248 Cm elemental ratios are respectively 6.1% and 3.2%.This level of uncertainty for both isotopic and elemental ratios is in perfect agreement with the requirement for transmutation studies. (authors)

  6. High-level viruria as a screening tool for BK virus nephropathy in renal transplant recipients

    Directory of Open Access Journals (Sweden)

    W. James Chon

    2016-09-01

    Conclusion: The presence of high-grade viruria is an early marker for developing BK viremia/BKVN. Detection of high-grade viruria should prompt early allograft biopsy and/or preemptive reduction in immunosuppression.

  7. Time-resolved resonance Raman spectroscopy of intermediates of bacteriorhodopsin: The bK(590) intermediate.

    Science.gov (United States)

    Terner, J; Hsieh, C L; Burns, A R; El-Sayed, M A

    1979-07-01

    We have combined microbeam and flow techniques with computer subtraction methods to obtain the resonance Raman spectrum of the short lived batho-intermediate (bK(590)) of bacteriorhodopsin. Comparison of the spectra obtained in (1)H(2)O and (2)H(2)O, as well as the fact that the bK(590) intermediate shows large optical red shifts, suggests that the Schiff base linkage of this intermediate is protonated. The fingerprint region of the spectrum of bK(590), sensitive to the isomeric configuration of the retinal chromophore, does not resemble the corresponding region of the parent bR(570) form. The resonance Raman spectrum of bK(590) as well as the spectra of all of the other main intermediates in the photoreaction cycle of bacteriorhodopsin are discussed and compared with resonance Raman spectra of published model compounds.

  8. Role of BK channels in the apoptotic volume decrease in native eel intestinal cells

    DEFF Research Database (Denmark)

    Lionetto, Maria Giulia; Giordano, Maria Elena; Calisi, Antonio

    2010-01-01

    High conductance Ca(+)-activated K(+) channels (BK channels) have previously been demonstrated in the eel intestine. They are specifically activated following a hypotonic stress and sustain Regulatory Volume Decrease (RVD). The aim of the present work was to address the possible role...... enterocytes that BK channels, which are involved in RVD in these cells, plays also a crucial role in the AVD process and in the progression of apoptosis....

  9. Hydronephrosis Resulting from Bilateral Ureteral Stenosis: A Late Complication of Polyoma BK Virus Cystitis?

    Science.gov (United States)

    Basara, N; Rasche, F-M; Schwalenberg, T; Wickenhauser, C; Maier, M; Ivovic, J; Niederwieser, D; Lindner, T H

    2010-01-01

    We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis probably due to polyoma BK virus-induced proliferation of bladder endothelium on both ostii. The diagnosis was made virologically by BK virus Polymerase Chain Reaction (PCR) detection in the absence of any other bladder disease. Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell transplantation from matched unrelated donor.

  10. Hydronephrosis Resulting from Bilateral Ureteral Stenosis: A Late Complication of Polyoma BK Virus Cystitis?

    Science.gov (United States)

    Basara, N.; Rasche, F.-M.; Schwalenberg, T.; Wickenhauser, C.; Maier, M.; Ivovic, J.; Niederwieser, D.; Lindner, T. H.

    2010-01-01

    We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis probably due to polyoma BK virus-induced proliferation of bladder endothelium on both ostii. The diagnosis was made virologically by BK virus Polymerase Chain Reaction (PCR) detection in the absence of any other bladder disease. Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell transplantation from matched unrelated donor. PMID:20936157

  11. Hydronephrosis Resulting from Bilateral Ureteral Stenosis: A Late Complication of Polyoma BK Virus Cystitis?

    OpenAIRE

    Basara, N.; Rasche, F.-M.; Schwalenberg, T.; Wickenhauser, C.; Maier, M.; Ivovic, J.; Niederwieser, D.; Lindner, T. H.

    2010-01-01

    We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis probably due to polyoma BK virus-induced proliferation of bladder endothelium on both ostii. The diagnosis was made virologically by BK virus Polymerase Chain Reaction (PCR) detection in the absence of any other bladder disease. Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell tra...

  12. Diagnosis of BK viral nephropathy in the renal allograft biopsy: role of fluorescence in situ hybridization.

    Science.gov (United States)

    Wang, Zhen; Portier, Bryce P; Hu, Bo; Chiesa-Vottero, Andres; Myles, Jonathan; Procop, Gary W; Tubbs, Raymond R

    2012-09-01

    Early recognition of BK viral nephropathy is essential for successful management. Our aim in this study was to evaluate a novel fluorescence in situ hybridization (FISH) assay for detection of BK virus in renal transplant biopsies in the context of standard detection methods. Renal allograft biopsies (n = 108) were analyzed via H&E, immunohistochemistry (IHC) for simian virus 40, and FISH for BK virus. BK virus was detected in 16 (14.8%) cases by H&E, 13 (12%) cases by IHC, 18 (16.6%) cases by FISH, and 19 (17.6%) cases by real-time PCR; 24 of 108 showed a discrepancy in ≥1 testing modalities. Comparison of H&E, IHC, and FISH showed no statistical difference in detection of BK virus. However, performing comparisons between the different tissue-based assays in the context of plasma or urine real-time PCR results showed significant improvement in detection of BK by FISH over H&E (P = 0.02) but not IHC (P = 0.07). This novel FISH-based approach for BK virus identification in renal allograft biopsy tissue mirrored real-time PCR results and showed superior performance to detection of inclusions by H&E. Therefore, use of FISH for BK virus detection in the setting of renal allograft biopsy is a useful and sensitive detection method and could be adopted in any laboratory that currently performs FISH analysis. Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  13. Detección de candidatos a trasplante hepático (1990-1999 en el Hospital Pablo Tobón Uribe y determinación de la supervivencia

    Directory of Open Access Journals (Sweden)

    Gonzálo Correa Arango

    2001-04-01

    Full Text Available

    Determinar en los pacientes cirróticos que consultaron al HPTU
    (1990-1999 los potenciales candidatos a un trasplante hepático, cuáles fueron sus causas de ingreso y la supervivencia actual sin trasplante hepático. Adicionar estos resultados a los obtenidos en el HUSVP para conocer la dinámica del comportamiento del paciente con enfermedad hepática susceptible de un trasplante hepático en la ciudad de Medellín.

     

     

  14. Reanálisis del estudio ESHOL: mortalidad por todas las causas considerando riesgos de competición y tiempo-dependientes para trasplante renal

    Directory of Open Access Journals (Sweden)

    Francisco Maduell

    2016-03-01

    Conclusiones: Los resultados del reanálisis del estudio ESHOL se confirman cuando se aplica el análisis en la población por intención de tratar sin censurar ninguna observación y considerando la mortalidad por todas las causas dependiente del tiempo y del riesgo competitivo del trasplante renal.

  15. Dieciséis años de experiencia de trasplante hepático en clínica las condes

    Directory of Open Access Journals (Sweden)

    G. Erwin Buckel, Dr.

    2010-03-01

    Conclusión: Los resultados obtenidos por el equipo de trasplante de Clínica Las Condes son en todo comparables a las series publicadas por centros internacionales y confirman la utilidad de esta terapia a nivel nacional. Aún existen nuevos desafíos que vencer en términos de inmunosupresión, infecciones y adherencia al tratamiento.

  16. Resultados funcionales de la procuración corneal con fines de trasplante realizado por un licenciado en Optometría

    Directory of Open Access Journals (Sweden)

    Oswaldo Manuel Aguirre-Luna

    2017-01-01

    Conclusiones: Todos los pacientes mejoraron visualmente con rechazo similar a lo reportado en otros estudios. Un licenciado en Optometría capacitado podría ser el profesional de salud idóneo para realizar procuraciones corneales con fines de trasplante.

  17. 149. Uso del oxigenador de membrana extracorpórea en el perioperatorio de trasplante pulmonar. Análisis de dos casos en nuestro centro

    Directory of Open Access Journals (Sweden)

    J.A. Fernández-Divar

    2012-04-01

    Conclusiones: El sistema ECMO es una herramienta válida para sustituir la CEC y disminuir sus riesgos en el trasplante pulmonar. Se puede mantener ECMO durante el postoperatorio precoz, sobre todo si se trata de pulmones de donante subóptimo o en casos de receptores de riesgo, especialmente aquellos con hipertensión pulmonar grave.

  18. The polyomavirus BK agnoprotein co-localizes with lipid droplets

    International Nuclear Information System (INIS)

    Unterstab, Gunhild; Gosert, Rainer; Leuenberger, David; Lorentz, Pascal; Rinaldo, Christine H.; Hirsch, Hans H.

    2010-01-01

    Agnoprotein encoded by human polyomavirus BK (BKV) is a late cytoplasmic protein of 66 amino acids (aa) of unknown function. Immunofluorescence microscopy revealed a fine granular and a vesicular distribution in donut-like structures. Using BKV(Dunlop)-infected or agnoprotein-transfected cells, we investigated agnoprotein co-localization with subcellular structures. We found that agnoprotein co-localizes with lipid droplets (LD) in primary human renal tubular epithelial cells as well as in other cells supporting BKV replication in vitro (UTA, Vero cells). Using agnoprotein-enhanced green fluorescent protein (EGFP) fusion constructs, we demonstrate that agnoprotein aa 20-42 are required for targeting LD, whereas aa 1-20 or aa 42-66 were not. Agnoprotein aa 22-40 are predicted to form an amphipathic helix, and mutations A25D and F39E, disrupting its hydrophobic domain, prevented LD targeting. However, changing the phosphorylation site serine-11 to alanine or aspartic acid did not alter LD co-localization. Our findings provide new clues to unravel agnoprotein function.

  19. Pharmacological consequences of the coexpression of BK channel α and auxiliary β subunits

    Science.gov (United States)

    Torres, Yolima P.; Granados, Sara T.; Latorre, Ramón

    2014-01-01

    Coded by a single gene (Slo1, KCM) and activated by depolarizing potentials and by a rise in intracellular Ca2+ concentration, the large conductance voltage- and Ca2+-activated K+ channel (BK) is unique among the superfamily of K+ channels. BK channels are tetramers characterized by a pore-forming α subunit containing seven transmembrane segments (instead of the six found in voltage-dependent K+ channels) and a large C terminus composed of two regulators of K+ conductance domains (RCK domains), where the Ca2+-binding sites reside. BK channels can be associated with accessory β subunits and, although different BK modulatory mechanisms have been described, greater interest has recently been placed on the role that the β subunits may play in the modulation of BK channel gating due to its physiological importance. Four β subunits have currently been identified (i.e., β1, β2, β3, and β4) and despite the fact that they all share the same topology, it has been shown that every β subunit has a specific tissue distribution and that they modify channel kinetics as well as their pharmacological properties and the apparent Ca2+ sensitivity of the α subunit in different ways. Additionally, different studies have shown that natural, endogenous, and synthetic compounds can modulate BK channels through β subunits. Considering the importance of these channels in different pathological conditions, such as hypertension and neurological disorders, this review focuses on the mechanisms by which these compounds modulate the biophysical properties of BK channels through the regulation of β subunits, as well as their potential therapeutic uses for diseases such as those mentioned above. PMID:25346693

  20. Pharmacological consequences of the coexpression of BK channel α and auxiliary β subunits

    Directory of Open Access Journals (Sweden)

    Yolima P. Torres

    2014-10-01

    Full Text Available Coded by a single gene (Slo1, KCM and activated by depolarizing potentials and by a rise in intracellular Ca2+ concentration, the large conductance voltage- and Ca+2-activated K+ channel (BK is unique among the superfamily of K+ channels. BK channels are tetramers characterized by a pore-forming α subunit containing seven transmembrane segments (instead of the six found in voltage-dependent K+ channels and a large C terminus composed of two regulators of K+ conductance domains (RCK domains, where the Ca2+-binding sites reside. BK channels can be associated with accessory β subunits and, although different BK modulatory mechanisms have been described, greater interest has recently been placed on the role that the β subunits may play in the modulation of BK channel gating due to its physiological importance. Four β subunits have currently been identified (i.e., β1, β2, β3 & β4 and despite the fact that they all share the same topology, it has been shown that every β subunit has a specific tissue distribution and that they modify channel kinetics as well as their pharmacological properties and the apparent Ca+2 sensitivity of the α subunit in different ways. Additionally, different studies have shown that natural, endogenous and synthetic compounds can modulate BK channels through β subunits. Considering the importance of these channels in different pathological conditions, such as hypertension and neurological disorders, this review focuses on the mechanisms by which these compounds modulate the biophysical properties of BK channels through the regulation of β subunits, as well as their potential therapeutic uses for diseases such as those mentioned above.

  1. Pharmacological consequences of the coexpression of BK channel α and auxiliary β subunits.

    Science.gov (United States)

    Torres, Yolima P; Granados, Sara T; Latorre, Ramón

    2014-01-01

    Coded by a single gene (Slo1, KCM) and activated by depolarizing potentials and by a rise in intracellular Ca(2+) concentration, the large conductance voltage- and Ca(2+)-activated K(+) channel (BK) is unique among the superfamily of K(+) channels. BK channels are tetramers characterized by a pore-forming α subunit containing seven transmembrane segments (instead of the six found in voltage-dependent K(+) channels) and a large C terminus composed of two regulators of K(+) conductance domains (RCK domains), where the Ca(2+)-binding sites reside. BK channels can be associated with accessory β subunits and, although different BK modulatory mechanisms have been described, greater interest has recently been placed on the role that the β subunits may play in the modulation of BK channel gating due to its physiological importance. Four β subunits have currently been identified (i.e., β1, β2, β3, and β4) and despite the fact that they all share the same topology, it has been shown that every β subunit has a specific tissue distribution and that they modify channel kinetics as well as their pharmacological properties and the apparent Ca(2+) sensitivity of the α subunit in different ways. Additionally, different studies have shown that natural, endogenous, and synthetic compounds can modulate BK channels through β subunits. Considering the importance of these channels in different pathological conditions, such as hypertension and neurological disorders, this review focuses on the mechanisms by which these compounds modulate the biophysical properties of BK channels through the regulation of β subunits, as well as their potential therapeutic uses for diseases such as those mentioned above.

  2. Large conductance Ca2+-activated K+ (BK channel: Activation by Ca2+ and voltage

    Directory of Open Access Journals (Sweden)

    RAMÓN LATORRE

    2006-01-01

    Full Text Available Large conductance Ca2+-activated K+ (BK channels belong to the S4 superfamily of K+ channels that include voltage-dependent K+ (Kv channels characterized by having six (S1-S6 transmembrane domains and a positively charged S4 domain. As Kv channels, BK channels contain a S4 domain, but they have an extra (S0 transmembrane domain that leads to an external NH2-terminus. The BK channel is activated by internal Ca2+, and using chimeric channels and mutagenesis, three distinct Ca2+-dependent regulatory mechanisms with different divalent cation selectivity have been identified in its large COOH-terminus. Two of these putative Ca2+-binding domains activate the BK channel when cytoplasmic Ca2+ reaches micromolar concentrations, and a low Ca2+ affinity mechanism may be involved in the physiological regulation by Mg2+. The presence in the BK channel of multiple Ca2+-binding sites explains the huge Ca2+ concentration range (0.1 μM-100 μM in which the divalent cation influences channel gating. BK channels are also voltage-dependent, and all the experimental evidence points toward the S4 domain as the domain in charge of sensing the voltage. Calcium can open BK channels when all the voltage sensors are in their resting configuration, and voltage is able to activate channels in the complete absence of Ca2+. Therefore, Ca2+ and voltage act independently to enhance channel opening, and this behavior can be explained using a two-tiered allosteric gating mechanism.

  3. Función de riesgo para la supervivencia en pacientes con trasplante cardiaco Risk function for survival in patients with heart transplant

    Directory of Open Access Journals (Sweden)

    Juan C Jaramillo

    2007-02-01

    Full Text Available Después de 20 años de experiencia y de 193 trasplantes cardiacos realizados, utilizando el modelo de riesgos proporcionales de Cox, fue posible evaluar las diferentes variables de riesgo, tanto pre como intra y post-operatorias, para construir un modelo de riesgo para la población sometida al procedimiento quirúrgico. Para determinar la significancia de cada una de las variables, se utilizó la ecuación de Breslow. Las variables identificadas como «de riesgo» fueron: enfermedad ácido-péptica, falla hepática, historia de tabaquismo, cirugía previa que comprometiera el pericardio, tiempo de ventilación prolongado, técnica quirúrgica de implantación, edad, sangrado asociado con anticoagulación y tiempo de inicio de la ciclosporina. Finalmente, las variables incluidas en el modelo de Cox fueron: presencia de enfermedad hepática, edad y tiempo de ventilación (mayor o igual a 48 horas. El modelo final es el siguiente: ht = h0(t Exp (1.377 x enf. hepática + 1.214 x t. de ventilación (0 60 añosAfter 20 years of experience with 193 heart transplants using the Cox model of proportional risks, it was possible to evaluate the different risk variables, both pre, intra and post-operatory, in order to construct a risk model for the population undergoing the surgical procedure. For determining the significance of each one of the variables, the Breslow equation was utilized. The variables identified as risky, were: peptic acid disease, liver failure, cigarette smoking, previous surgery with pericardial involvement, prolonged ventilation time, surgical implantation technique, age, bleeding associated to anticoagulation and cyclosporine starting time. Finally, the variables included in the Cox model were: presence of liver disease, age and ventilation time (greater or equal to 48 hours. The final model is the following: Ht = ho(t Exp (1.377 x hepatic disease + 1.214 x ventilation time (0 60 years].

  4. Cholesterol tuning of BK ethanol response is enantioselective, and is a function of accompanying lipids.

    Directory of Open Access Journals (Sweden)

    Chunbo Yuan

    Full Text Available In the search to uncover ethanol's molecular mechanisms, the calcium and voltage activated, large conductance potassium channel (BK has emerged as an important molecule. We examine how cholesterol content in bilayers of 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE/sphingomyelin (SPM and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylethanolamine (POPE/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylserine (POPS affect the function and ethanol sensitivity of BK. In addition, we examine how manipulation of cholesterol in biological membranes modulates ethanol's actions on BK. We report that cholesterol levels regulate the change in BK channel open probability elicited by 50 mM ethanol. Low levels of cholesterol (<20%, molar ratio supports ethanol activation, while high levels of cholesterol leads to ethanol inhibition of BK. To determine if cholesterol affects BK and its sensitivity to ethanol through a direct cholesterol-protein interaction or via an indirect action on the lipid bilayer, we used the synthetic enantiomer of cholesterol (ent-CHS. We found that 20% and 40% ent-CHS had little effect on the ethanol sensitivity of BK, when compared with the same concentration of nat-CHS. We accessed the effects of ent-CHS and nat-CHS on the molecular organization of DOPE/SPM monolayers at the air/water interface. The isotherm data showed that ent-CHS condensed DOPE/SPM monolayer equivalently to nat-CHS at a 20% concentration, but slightly less at a 40% concentration. Atomic force microscopy (AFM images of DOPE/SPM membranes in the presence of ent-CHS or nat-CHS prepared with LB technique or vesicle deposition showed no significant difference in topographies, supporting the interpretation that the differences in actions of nat-CHS and ent-CHS on BK channel are not likely from a generalized action on bilayers. We conclude that membrane cholesterol influences ethanol's modulation of BK in a complex manner, including an interaction with the channel protein

  5. Aspectos ético legales del trasplante de corazón Ethical and legal aspects of heart transplant

    Directory of Open Access Journals (Sweden)

    Fernán del C Mendoza

    2007-12-01

    Full Text Available El trasplante cardiaco constituye el tratamiento de elección para los pacientes en falla cardiaca refractaria. Desde el punto de vista médico, existen claras indicaciones para realizar el trasplante, pero a nivel local y mundial hay problemas por el escaso número de donantes. Para que se realice un trasplante de corazón, el donante debe reunir criterios de muerte encefálica, la cual se diagnostica por ausencia irreversible de las funciones del tallo encefálico, determinadas por un examen clínico. A pesar de las controversias que existen acerca de cuándo muere una persona, cada vez hay más claridad en este hecho. Existen otros problemas bioéticos que se relacionan con el trasplante como la donación (algunas personas en vida manifiestan su voluntad y autonomía de servir como donantes y otras no, la distribución de órganos y el comercio de órganos y tejidos. Desde el punto de vista legal y ético, se prohíbe la gratificación o pago al donante vivo, a la familia del donante fallecido, al banco de tejidos o de médula ósea, a las clínicas y hospitales, y a las aseguradoras por la donación o suministro de órganos o tejidos humanos. La promoción de la donación y la obtención de componentes anatómicos deberán efectuarse denotando su carácter voluntario, altruista y desinteresado. Debe primar el principio de justicia y excluirse cualquier consideración no equitativa de índole geográfica, racial, sexual, religiosa, etc. Los órganos deben distribuirse con base en criterios médicos; así mismo debe buscarse la más idónea utilización del órgano donado, teniendo como fundamento el manejo equitativo y como principio el respeto por la vida y la dignidad humana.Cardiac transplant is the treatment of choice for patients with refractory cardiac failure. There are clear indications from the medical point of view to perform the transplant, but locally and world - wide there are problems due to the scarce number of donors. In order

  6. Impact of two different commercial DNA extraction methods on BK virus viral load

    Directory of Open Access Journals (Sweden)

    Massimiliano Bergallo

    2016-03-01

    Full Text Available Background and aim: BK virus, a member of human polyomavirus family, is a worldwide distributed virus characterized by a seroprevalence rate of 70-90% in adult population. Monitoring of viral replication is made by evaluation of BK DNA by quantitative polymerase chain reaction. Many different methods can be applied for extraction of nucleic acid from several specimens. The aim of this study was to assess the impact of two different DNA extraction procedure on BK viral load. Materials and methods: DNA extraction procedure including the Nuclisens easyMAG platform (bioMerieux, Marcy l’Etoile, France and manual QIAGEN extraction (QIAGEN Hilden, Germany. BK DNA quantification was performed by Real Time TaqMan PCR using a commercial kit. Result and discussion: The samples capacity, cost and time spent were compared for both systems. In conclusion our results demonstrate that automated nucleic acid extraction method using Nuclisense easyMAG was superior to manual protocol (QIAGEN Blood Mini kit, for the extraction of BK virus from serum and urine specimens.

  7. Registro Español de Trasplante Cardíaco. XX Informe oficial de la sección de insuficiencia Cardíaca y Trasplante Cardíaco de la sociedad Española de Cardiología (1984-2008

    Directory of Open Access Journals (Sweden)

    Luis Almenar Bonet

    2009-07-01

    Conclusiones: La supervivencia obtenida en España con el TC, sobre todo en los últimos años, sitúa al trasplante cardíaco como el tratamiento de elección para cardiopatías irreversibles en situación funcional avanzada y sin otras opciones médicas o quirúrgicas establecidas.

  8. Molecular Mechanisms Underlying Renin-Angiotensin-Aldosterone System Mediated Regulation of BK Channels

    Directory of Open Access Journals (Sweden)

    Zhen-Ye Zhang

    2017-09-01

    Full Text Available Large-conductance calcium-activated potassium channels (BK channels belong to a family of Ca2+-sensitive voltage-dependent potassium channels and play a vital role in various physiological activities in the human body. The renin-angiotensin-aldosterone system is acknowledged as being vital in the body's hormone system and plays a fundamental role in the maintenance of water and electrolyte balance and blood pressure regulation. There is growing evidence that the renin-angiotensin-aldosterone system has profound influences on the expression and bioactivity of BK channels. In this review, we focus on the molecular mechanisms underlying the regulation of BK channels mediated by the renin-angiotensin-aldosterone system and its potential as a target for clinical drugs.

  9. Cholesterol Down-Regulates BK Channels Stably Expressed in HEK 293 Cells

    Science.gov (United States)

    Deng, Xiu-Ling; Sun, Hai-Ying; Li, Gui-Rong

    2013-01-01

    Cholesterol is one of the major lipid components of the plasma membrane in mammalian cells and is involved in the regulation of a number of ion channels. The present study investigates how large conductance Ca2+-activated K+ (BK) channels are regulated by membrane cholesterol in BK-HEK 293 cells expressing both the α-subunit hKCa1.1 and the auxiliary β1-subunit or in hKCa1.1-HEK 293 cells expressing only the α-subunit hKCa1.1 using approaches of electrophysiology, molecular biology, and immunocytochemistry. Membrane cholesterol was depleted in these cells with methyl-β-cyclodextrin (MβCD), and enriched with cholesterol-saturated MβCD (MβCD-cholesterol) or low-density lipoprotein (LDL). We found that BK current density was decreased by cholesterol enrichment in BK-HEK 293 cells, with a reduced expression of KCa1.1 protein, but not the β1-subunit protein. This effect was fully countered by the proteasome inhibitor lactacystin or the lysosome function inhibitor bafilomycin A1. Interestingly, in hKCa1.1-HEK 293 cells, the current density was not affected by cholesterol enrichment, but directly decreased by MβCD, suggesting that the down-regulation of BK channels by cholesterol depends on the auxiliary β1-subunit. The reduced KCa1.1 channel protein expression was also observed in cultured human coronary artery smooth muscle cells with cholesterol enrichment using MβCD-cholesterol or LDL. These results demonstrate the novel information that cholesterol down-regulates BK channels by reducing KCa1.1 protein expression via increasing the channel protein degradation, and the effect is dependent on the auxiliary β1-subunit. PMID:24260325

  10. CALIDAD DE VIDA Y ESTRATEGIAS DE AFRONTAMIENTO EN PACIENTES CON INSUFICIENCIA RENAL CRÓNICA SOMETIDOS A HEMODIÁLISIS, DIÁLISIS PERITONEAL Y TRASPLANTE RENAL

    Directory of Open Access Journals (Sweden)

    Cesar Armando Rey Anacona

    2008-07-01

    Full Text Available Se comparó la calidad de vida y las estrategias de afrontamiento en 120 pacientes con Insuficiencia Renal Crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal, teniendo en cuenta la edad, sexo, escolaridad, estado civil y ocupación. Los instrumentos utilizados fueron una ficha sociodemográfica, la prueba de calidad de vida WHOQOL-100 (Organización Mundial de la Salud, 1998 y el Cuestionario de Estrategias de Afrontamiento (Lazarus & Folkman, 1985. Se encontró que los pacientes en tratamiento de trasplante reportaban mayor calidad de vida y mejores estrategias de afrontamiento en comparación con los otros tratamientos, siendo mayores las puntuaciones en personas con edad de transición entre adultez media y tardía, mujeres, con estudios universitarios, casados y empleados. Se discuten las implicaciones de estos hallazgos.

  11. Hydronephrosis Resulting from Bilateral Ureteral Stenosis: A Late Complication of Polyoma BK Virus Cystitis?

    Directory of Open Access Journals (Sweden)

    N. Basara

    2010-01-01

    Full Text Available We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis probably due to polyoma BK virus-induced proliferation of bladder endothelium on both ostii. The diagnosis was made virologically by BK virus Polymerase Chain Reaction (PCR detection in the absence of any other bladder disease. Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell transplantation from matched unrelated donor.

  12. Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat

    DEFF Research Database (Denmark)

    Poulsen, Asser Nyander; Jansen-Olesen, Inger; Olesen, Jes

    2011-01-01

    The big conductance calcium-activated K(+) channel (BK) is involved in regulating neuron and smooth muscle cell excitability. Functional diversity of BK is generated by alpha-subunit splice variation and co-expression with beta subunits. Here, we present six different splice combinations cloned...... and RCK2 (4 aa at SS1) and upstream of the calcium "bowl" (27 aa at SS4). Two other truncated variants, X2(92) and X2(188), lacking the intracellular C-terminal (stop downstream of S6), were cloned from cerebral vascular/meningeal tissue. They appear non-functional as no current expression was observed...

  13. Implementación y resultados de un nuevo programa de ECMO para trasplante de pulmón y distrés respiratorio agudo

    Directory of Open Access Journals (Sweden)

    Eduardo San Roman

    2015-06-01

    Full Text Available RESUMEN Objetivo: El desarrollo de la membrana de oxigenación extracorpórea en América Latina representa un desafío para la especialidad. El objetivo de este artículo fue describir los resultados de un nuevo programa de membrana de oxigenación extracorpórea en una unidad de cuidados intensivos. Métodos: Estudio de cohorte retrospectivo. Incluye 22 pacientes que requirieron membrana de oxigenación extracorpórea desde Enero de 2011 hasta Junio de 2014. Se evaluaron características basales, indicaciones, duración de la corrida, días de ventilación mecánica, días de unidad de cuidados intensivos, complicaciones y mortalidad hospitalaria. Resultados: Quince pacientes requirieron membrana de oxigenación extracorpórea post-trasplante pulmonar y 7 pacientes por distrés respiratorio agudo. Todos los pacientes trasplantados fueron destetados de membrana de oxigenación extracorpórea, con una duración mediana de 3 días (Rango intercuantil - IQR: 2 - 5, de ventilación mecánica 15,5 días (IQR: 3 - 35, de estadía unidad de cuidados intensivos 31,5 días (IQR: 19 - 53 y de estadía hospitalaria 60 días (IQR: 36 - 89, con una mortalidad de 20%. Los pacientes con distrés respiratorio agudo tuvieron una mediana de duración de membrana de oxigenación extracorpórea de 9 días (IQR: 3 - 14, mediana de ventilación mecánica 25 días (IQR: 13 - 37, de estadía en terapia 31 días (IQR: 11 - 38, y hospitalaria 32 días (IQR: 11 - 41, y 57% de mortalidad. Las principales complicaciones fueron infecciones (80%, insuficiencia renal aguda (43%, sangrados en sitio quirúrgico y de inserción de cánulas (22%, plaquetopenia (60% y coagulopatía (30%. Conclusión: A pesar de encontrarnos transitando una curva de aprendizaje, consideramos la experiencia satisfactoria, con resultados y complicaciones comparables a las reportadas en la literatura.

  14. Molecular investigations of BK(Ca) channels and the modulatory beta-subunits in porcine basilar and middle cerebral arteries

    DEFF Research Database (Denmark)

    Johansson, Helle Wulf; Hay-Schmidt, Anders; Poulsen, Asser Nyander

    2009-01-01

    arteries using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Western blotting was used to detect immunoreactivity for the porcine BK(Ca) channel alpha-subunit and beta-subunit proteins. The BK(Ca) channel alpha-subunit RNA and protein distribution patterns were......Large conductance calcium-activated potassium (BK(Ca)) channels are fundamental in the regulation of cerebral vascular basal tone. We investigated the expression of the mRNA transcripts for the BK(Ca) channel and its modulatory beta-subunits (beta1-beta4) in porcine basilar and middle cerebral...... visualized using in situ hybridization and immunofluorescence studies, respectively. The study verified that the BK(Ca) channel alpha-subunit is located to smooth muscle cells of porcine basilar and middle cerebral arteries. The mRNA transcript for beta1-, beta2- and beta4-subunit were shown by RT...

  15. Outcomes of renal transplant recipients with BK virus infection and BK virus surveillance in the Auckland region from 2006 to 2012.

    Science.gov (United States)

    Hsiao, Chun-Yuan; Pilmore, Helen L; Zhou, Lifeng; de Zoysa, Janak R

    2016-11-06

    To evaluate incidence, risk factors and treatment outcome of BK polyomavirus nephropathy (BKVN) in a cohort of renal transplant recipients in the Auckland region without a formal BK polyomavirus (BKV) surveillance programme. A cohort of 226 patients who received their renal transplants from 2006 to 2012 was retrospectively reviewed. Seventy-six recipients (33.6%) had a BK viral load (BKVL) test and 9 patients (3.9%) developed BKVN. Cold ischaemia time (HR = 1.18, 95%CI: 1.04-1.35) was found to be a risk factor for BKVN. Four recipients with BKVN had complete resolution of their BKV infection; 1 recipient had BKVL less than 625 copies/mL; 3 recipients had BKVL more than 1000 copies/mL and 1 had graft failure from BKVN. BKVN has a negative impact on graft function [median estimated glomerular filtration rate (eGFR) 22.5 (IQR 18.5-53.0) mL/min per 1.73 m 2 , P = 0.015), but no statistically significant difference ( P = 0.374) in renal allograft function was found among negative BK viraemia group [median eGFR 60.0 (IQR 48.5-74.2) mL/min per 1.73 m 2 ), positive BK viraemia without BKVN group [median eGFR 55.0 (IQR 47.0-76.0) mL/min per 1.73 m 2 ] and unknown BKV status group [median eGFR 54.0 (IQR 43.8-71.0) mL/min per 1.73 m 2 ]. The incidence and treatment outcomes of BKVN were similar to some centres with BKV surveillance programmes. Recipients with BVKN have poorer graft function. Although active surveillance for BKV has been shown to be effective in reducing incidence of BKVN, it should be tailored specifically to that transplant centre based on its epidemiology and outcomes of BKVN, particularly in centres with limited resources.

  16. Molecular mapping of qBK1 WD , a major QTL for bakanae disease resistance in rice.

    Science.gov (United States)

    Lee, Sais-Beul; Hur, Yeon-Jae; Cho, Jun-Hyeon; Lee, Jong-Hee; Kim, Tae-Heon; Cho, Soo-Min; Song, You-Chun; Seo, Young-Su; Lee, Jungkwan; Kim, Tae-Sung; Park, Yong-Jin; Oh, Myung-Kyu; Park, Dong-Soo

    2018-01-10

    Bakanae or foot rot disease is a prominent disease of rice caused by Gibberella fujikuroi. This disease may infect rice plants from the pre-emergence stage to the mature stage. In recent years, raising rice seedlings in seed boxes for mechanical transplanting has increased the incidence of many seedling diseases; only a few rice varieties have been reported to exhibit resistance to bakanae disease. In this study, we attempted to identify quantitative trait loci (QTLs) conferring bakanae disease resistance from the highly resistant japonica variety Wonseadaesoo. A primary QTL study using the genotypes/phenotypes of the recombinant inbred lines (RILs) indicated that the locus qBK1 WD conferring resistance to bakanae disease from Wonseadaesoo was located in a 1.59 Mb interval delimited on the physical map between chr01_13542347 (13.54 Mb) and chr01_15132528 (15.13 Mb). The log of odds (LOD) score of qBK1 WD was 8.29, accounting for 20.2% of the total phenotypic variation. We further identified a gene pyramiding effect of two QTLs, qBK WD and previously developed qBK1. The mean proportion of healthy plant for 31 F 4 RILs that had no resistance genes was 35.3%, which was similar to that of the susceptible check variety Ilpum. The proportion of healthy plants for the lines with only qBK WD or qBK1 was 66.1% and 55.5%, respectively, which was significantly higher than that of the lines without resistance genes and that of Ilpum. The mean proportion of the healthy plant for 15 F 4 RILs harboring both qBK WD and qBK1 was 80.2%, which was significantly higher than that of the lines with only qBK WD or qBK1. Introducing qBK WD or pyramiding the QTLs qBK WD and qBK1 could provide effective tools for breeding rice with bakanae disease resistance. To our knowledge, this is the first report on a gene pyramiding effect that provides higher resistance against bakanae disease.

  17. KCNMA1 encoded cardiac BK channels afford protection against ischemia-reperfusion injury

    DEFF Research Database (Denmark)

    Soltysinska, Ewa; Bentzen, Bo Hjorth; Barthmes, Maria

    2014-01-01

    Mitochondrial potassium channels have been implicated in myocardial protection mediated through pre-/postconditioning. Compounds that open the Ca2+- and voltage-activated potassium channel of big-conductance (BK) have a pre-conditioning-like effect on survival of cardiomyocytes after ischemia/rep...

  18. Identification of the new isotope sup 2 sup 4 sup 1 Bk

    CERN Document Server

    Asai, M; Ichikawa, S; Nagame, Y; Nishinaka, I; Akiyama, K; Toyoshima, A; Kaneko, T; Sakama, M; Haba, H; Oura, Y; Kojima, Y; Shibata, M

    2003-01-01

    A new neutron-deficient berkelium isotope sup 2 sup 4 sup 1 Bk produced in the sup 2 sup 3 sup 9 Pu( sup 6 Li, 4n) reaction has been identified using a gas-jet coupled on-line isotope separator. Cm K and L X-rays associated with the EC decay of sup 2 sup 4 sup 1 Bk were observed in the mass-241 fraction, and three gamma transitions were attributed to the EC decay of sup 2 sup 4 sup 1 Bk through X-gamma coincidences. The half-life of sup 2 sup 4 sup 1 Bk was determined to be 4.6+-0.4 min which is 1/2-1/4 of that of theoretical predictions. The half-life value and the observed gamma transitions can be consistently explained as a consequence of the allowed EC transition of pi 7/2 sup + [633] -> nu 7/2 sup + [624]. (orig.)

  19. Differential expression of BK channel isoforms and beta-subunits in rat neuro-vascular tissues

    DEFF Research Database (Denmark)

    Poulsen, Asser Nyander; Wulf, Helle; Hay-Schmidt, Anders

    2009-01-01

    We investigated the expression of splice variants and beta-subunits of the BK channel (big conductance Ca(2+)-activated K(+) channel, Slo1, MaxiK, K(Ca)1.1) in rat cerebral blood vessels, meninges, trigeminal ganglion among other tissues. An alpha-subunit splice variant X1(+24) was found expresse...

  20. Vaccine for BK Polyomavirus-associated Infections in Transplant Recipients | NCI Technology Transfer Center | TTC

    Science.gov (United States)

    NCI researches identified a BK polyomavirus (BKV) virulent strain that causes chronic urinary tract infections, and the development of vaccine and therapeutic methods that would block BKV pathogenesis. The NCI Laboratory of Cellular Oncology, seek parties to license or co-develop this technology.

  1. Persepsi Guru BK Tentang Kompetensi Konselor di Sekolah Dasar Swasta Kota Semarang

    Directory of Open Access Journals (Sweden)

    Restu Setyoningtyas

    2014-10-01

    Full Text Available Tujuan penelitian ini adalah untuk mengetahui tentang persepsi guru BK tentang kompetensi konselor di sekolah dasar swasta Kota Semarang. Penelitian ini bersifat kuantitatif, responden penelitian adalah guru BK sekolah dasar swasta, teknik pengumpulan data berupa skala psikologi dan dokumentasi. Analisis data menggunakan Analisis Deskriptif Persentase. Hasil penelitian menunjukkan persepsi guru BK tentang kompetensi konselor mendapatkan hasil yang positif. Kompetensi pedagogik yaitu positif, kepribadian yaitu kurang positif, sosial yaitu kurang positif, profesional yaitu cukup positif. Kesimpulan dari penelitian ini adalah persepsi guru BK tentang kompetensi konselor di sekolah dasar swasta Kota Semarang pada umumnya positif. The purpose of this research is determine perception guidance and counseling teacher about counselor competence in private elementary school on Semarang city. This research is quantitative. Respondens is guidance and counseling teacher from private elementary school, techniques of data collection are psychology scale and documentation. The data analysis using Analysis Descriptive Percentage. Result showed that research is perception  guidance and counseling teacher about counselor competence is positive. Pedagogic competence is positive, personality is not positive enough, social is not positive enough, professional is positive enough. The conclusion of this research is perception guidance and counseling teacher about counselor competence in private elementary school on Semarang city in general is positive.

  2. EL POLVO DE COCO COMO SUSTRATO ALTERNATIVO PARA LA OBTENCIÓN DE PLANTAS ORNAMENTALES PARA TRASPLANTE

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    A. Ayala-Sierra

    2008-01-01

    Full Text Available La presente investigación se realizó con el objeto de evaluar la factibilidad de utilizar sustratos elaborados a base de polvo de coco para la germinación de semillas y desarrollo inicial de plantas para trasplante de seis especies ornamentales y determinar la posibilidad de remplazar al Peat Moss. Las especies seleccionadas para el estudio fueron: Dianthus chinensis (clavelina, Gazania rigens (gazania, Tagetes erecta (marigold, Viola wittrockiana (pensamiento, Antirrhinum majus (dragón y Petunia x hybrida (petunia. Las semillas de las seis especies fueron sembradas en tres sustratos: polvo de coco, Peat Moss y Peat Lite. Previo a la siembra, el polvo de coco fue acondicionado mediante el ajuste del pH a 6.2, la adición de cal dolomítica y KNO3 como carga de nutrientes. Posteriormente, se elaboró una mezcla a base de polvo de coco, perlita y vermiculita a razón de 70, 20 y 10 % (v/v, y Peat Moss, perlita y vermiculita en las mismas proporciones. El Peat Lite contenía 50 % de Peat Moss y 50 % de perlita (v/v. Se evaluó el efecto de los sustratos en la altura de planta, el peso seco del vástago y de raíz, el porcentaje de germinación y el porcentaje de plantas con valor comercial. Aunque el polvo de coco causó una disminución significativa en la altura de plantas de gazania (21 %, dragones (30 % y petunias (13 %, y en el peso seco del vástago de dragones (24 % y en el peso seco de raíz de clavelina (22 %, esto no estuvo asociado con una disminución en la calidad de las plantas. Por lo tanto, se concluye que el polvo de coco puede utilizarse como sustrato para la producción de plantas para trasplante de especies ornamentales.

  3. Trasplante no mieloablativo de células hematopoyéticas Nonmyeloablative transplantation of haematopoietic cells

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    Valia Pavón Morán

    2003-12-01

    Full Text Available El trasplante de células hematopoyéticas es una opción terapéutica para muchas enfermedades. Los tratamientos preparatorios convencionales están asociados con una alta morbimortalidad por toxicidad sobre órganos y tejidos, lo que limita su indicación a individuos jóvenes y con un estado de salud competente. El papel beneficioso del efecto injerto contra tumor y ante la evidencia de inducción de una nueva remisión con infusión de linfocitos del donante, en pacientes que recayeron después de un trasplante alogénico, sentaron las bases para el desarrollo de tratamientos preparatorios menos agresivos, no mieloablativos, pero sí intensamente inmunosupresores, que permitieran lograr la toma del injerto y un estado de quimerismo mixto que pudiera ser modulado a un estado de quimera total. Los resultados demuestran que puede lograrse el implante después de un tratamiento no mieloablativo. Actualmente están por determinarse los esquemas ideales, así como la terapia inmunosupresora postraThe transplantation of haematopoietic cells is a therapeutic option for many diseases. The conventional preparatory treatments are associated with a high morbimortality due to toxicity over organs and tissues, which limits their indications to young individuals and with a competent health status.The beneficial role of the graft versus tumor effect and the evidence of induction of a new remission with infusion of lymphocytes from the donor in patients that relapsed after an allogeneic transplant, laid the foundations for the development of less aggressive and nonmyeloablative but intensively immunosupressor preparatory treatments that would allow the taking of the graft and a state of mixed chimerism that may be modulated by a state of total chimera. The results show that the implant may be attained after a nonmyeloablative treatment. At present, the ideal schemes, as well as the posttransplant immunosupressor therapy are to be determined

  4. Fragilidad y estado nutricional en el periodo pre-trasplante. ¿Existe relación?

    Directory of Open Access Journals (Sweden)

    Maria Vera Casanova

    Full Text Available Resumen Introducción: La malnutrición en la enfermedad renal crónica (ERC está asociada al aumento del riesgo de mortalidad y complicaciones. Sin embargo, el estado nutricional de los pacientes con ERC en lista de espera (LE de trasplante renal (TR, y su relación con el estado de fragilidad ha sido poco evaluado. Objetivo: Analizar el estado nutricional y funcional de una cohorte de pacientes en lista de espera de trasplante renal y su relación con la fragilidad. Pacientes y Método: Estudio retrospectivo de los pacientes incluidos en LE de TR desde Junio 2016 hasta Junio 2017. Se evaluaron a los pacientes mediante distintas escalas de valoración, recogiendo parámetros antropométricos, analíticos y de bioimpedanciometría (BCM. Resultados: De los 177 pacientes incluidos, 55 (31.1% se definieron como frágiles. Dicho grupo eran de mayor edad (64.2 vs 61.2 años; p=0.08, sexo femenino (56.4% vs 32.8%, p<0.01, IMC mayor (29.3±5.8 vs 27.3±5,6 kg/m²; p=0.03 y más frecuentemente diabéticos (DM2 (43.6% vs 30.3%; p=0.08. La evaluación mediante BCM demostró que los pacientes frágiles tenían menos índice de tejido magro (12.3±2.4 vs 14.1±4 kg/m²; p<0.01 y mayor índice de tejido graso (16.2±6.1 vs 11.8±5.6 kg/m²; p<0.01 que los no frágiles. Conclusión: En nuestra lista de espera de TR, los pacientes frágiles son de mayor edad, sexo femenino, mayor grado de obesidad, DMII y con menor masa muscular. Las estrategias encaminadas a liberalizar dietas, aumentar la ingesta proteica y fomentar el ejercicio físico pueden ser relevantes para mejorar los resultados a corto y largo plazo del TR.

  5. Surveillance of polyomavirus BK in relation to immunosuppressive therapy in kidney transplantation

    Directory of Open Access Journals (Sweden)

    Cristina Costa

    2012-03-01

    Full Text Available Introduction. Reactivation of polyomavirus BK in kidney transplant recipients has been associated to the development of nephropathy (polyomavirus-associated nephropathy, PVAN, possibly leading to the loss of the transplanted organ. Immunosuppression is the condicio sine qua non for the onset of PVAN; however, a lower incidence of BK viremia has been reported with low-level tacrolimus based immunosuppressive protocols in comparison to cyclosporine A.Aim of this study was to compare the two immunosuppressive protocols. Methods. Virological monitoring of BK was performed in 468 consecutive renal transplant patients over a period of 3 years (2370 urine e 2370 serum specimens: in particular, 1780 specimens from 362 patients treated with tacrolimus and 590 from 106 treated with cyclosporine A. Results. BK viremia was evidenced in 124 (7.0% and 12 (2.0% specimens from 40 (11.0% and 11 (10.4% patients treated with tacrolimus and cyclosporine A, respectively; similarly, BK viruria in 289 (16.2% and 58 (9.8% specimens from 67 (18.5% and 27 (25.5% patients, being the difference of incidence highly significant (p <0.0001 for both viremia and viruria at comparison between specimens and not significant for patients. No case of PVAN was diagnosed at histophatology evaluation. Conclusions. The incidence of viremia and viruria was similar to that previously reported. Our results evidenced that with low-level tacrolimus-based protocols the overall incidence of reactivation in renal transplant patients is not significantly different and there is no increased risk of PVAN, nevertheless the higher incidence of episodes of reactivation.

  6. The oncogenic potential of BK-polyomavirus is linked to viral integration into the human genome.

    Science.gov (United States)

    Kenan, Daniel J; Mieczkowski, Piotr A; Burger-Calderon, Raquel; Singh, Harsharan K; Nickeleit, Volker

    2015-11-01

    It has been suggested that BK-polyomavirus is linked to oncogenesis via high expression levels of large T-antigen in some urothelial neoplasms arising following kidney transplantation. However, a causal association between BK-polyomavirus, large T-antigen expression and oncogenesis has never been demonstrated in humans. Here we describe an investigation using high-throughput sequencing of tumour DNA obtained from an urothelial carcinoma arising in a renal allograft. We show that a novel BK-polyomavirus strain, named CH-1, is integrated into exon 26 of the myosin-binding protein C1 gene (MYBPC1) on chromosome 12 in tumour cells but not in normal renal cells. Integration of the BK-polyomavirus results in a number of discrete alterations in viral gene expression, including: (a) disruption of VP1 protein expression and robust expression of large T-antigen; (b) preclusion of viral replication; and (c) deletions in the non-coding control region (NCCR), with presumed alterations in promoter feedback loops. Viral integration disrupts one MYBPC1 gene copy and likely alters its expression. Circular episomal BK-polyomavirus gene sequences are not found, and the renal allograft shows no productive polyomavirus infection or polyomavirus nephropathy. These findings support the hypothesis that integration of polyomaviruses is essential to tumourigenesis. It is likely that dysregulation of large T-antigen, with persistent over-expression in non-lytic cells, promotes cell growth, genetic instability and neoplastic transformation. © 2015 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

  7. Faktor-Faktor Hambatan Profesionalisasi Guru BK di SMA Negeri se- Kota Purwokerto

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    Cahya Dewi Rizkiwati

    2014-10-01

    Full Text Available Tujuan penelitian ini yaitu untuk mendeskripsikan tentang faktor-faktor hambatan yang terjadi dalam profesionalisasi guru BK. Jenis penelitian ini merupakan penelitian survey. Penelitian ini dilaksanakan di SMA Negeri se-Kota Purwokerto. Penelitian ini adalah penelitian populasi atau sensus karena populasi guru BK berjumlah 25 orang. Metode yang digunakan dalam penelitian ini adalah  inventori dalam bentuk daftar cek masalah. Instrumen tersebut telah diuji validitasnya menggunakan rumus point biserial, sedangkan reliabilitas instrumen digunakan rumus KR-20. Data yang telah diperoleh dianalisis dengan menggunakan teknik deskriptif prosentase. Hasil dari penelitian ini menunjukkan hambatan yang berasal dari faktor internal mendapat prosentase lebih tinggi daripada faktor eksternal. Faktor internal yang paling mempengaruhi profesionalisasi guru BK antara lain latar belakang pendidikan, pengalaman kerja, motivasi kerja, kompetensi guru BK. Sedangkan faktor eksternal yang paling mempengaruhi profesionalisasi guru BK adalah sarana dan prasarana. The purpose of this research is to describe problems  occur in the professionalization of guidance and counseling teachers. The type of the research is survey research . This research was conducted in all of Senior High School in Purwokerto. Population research or census were used as sampling method, since  the population of guidance and counseling teachers were 25 people. Data collection technique was  using inventory with list of issues. The instrument validity has been tested using point biserial formula, whilst its reliability was tested using KR-20 formula. The data was analyzed using descriptive percentage techniques. The results of this research indicate that internal factors get higher percentage than external factors. Internal factors that have the most influence in the professionalization of guidance and counseling teachers include educational background, working experience , motivation, teacher

  8. Relative transmembrane segment rearrangements during BK channel activation resolved by structurally assigned fluorophore–quencher pairing

    Science.gov (United States)

    Pantazis, Antonios

    2012-01-01

    Voltage-activated proteins can sense, and respond to, changes in the electric field pervading the cell membrane by virtue of a transmembrane helix bundle, the voltage-sensing domain (VSD). Canonical VSDs consist of four transmembrane helices (S1–S4) of which S4 is considered a principal component because it possesses charged residues immersed in the electric field. Membrane depolarization compels the charges, and by extension S4, to rearrange with respect to the field. The VSD of large-conductance voltage- and Ca-activated K+ (BK) channels exhibits two salient inconsistencies from the canonical VSD model: (1) the BK channel VSD possesses an additional nonconserved transmembrane helix (S0); and (2) it exhibits a “decentralized” distribution of voltage-sensing charges, in helices S2 and S3, in addition to S4. Considering these unique features, the voltage-dependent rearrangements of the BK VSD could differ significantly from the standard model of VSD operation. To understand the mode of operation of this unique VSD, we have optically tracked the relative motions of the BK VSD transmembrane helices during activation, by manipulating the quenching environment of site-directed fluorescent labels with native and introduced Trp residues. Having previously reported that S0 and S4 diverge during activation, in this work we demonstrate that S4 also diverges from S1 and S2, whereas S2, compelled by its voltage-sensing charged residues, moves closer to S1. This information contributes spatial constraints for understanding the BK channel voltage-sensing process, revealing the structural rearrangements in a non-canonical VSD. PMID:22802360

  9. The BK(Ca) channels deficiency as a possible reason for radiation-induced vascular hypercontractility.

    Science.gov (United States)

    Kyrychenko, Sergii; Tishkin, Sergey; Dosenko, Victor; Ivanova, Irina; Novokhatska, Tatiana; Soloviev, Anatoly

    2012-01-01

    It is likely that large-conductance Ca²⁺-activated K⁺ (BK(Ca)) channels channelopathy tightly involved in vascular malfunctions and arterial hypertension development. In the present study, we compared the results of siRNAs-induced α-BK(Ca) gene silencing and vascular abnormalities produced by whole-body ionized irradiation in rats. The experimental design comprised RT-PCR and patch clamp technique, thoracic aorta smooth muscle (SM) contractile recordings and arterial blood pressure (BP) measurements on the 30th day after whole body irradiation (6Gy) and following siRNAs KCNMA1 gene silencing in vivo. The expression profile of BK(Ca) mRNA transcripts in SM was significantly decreased in siRNAs-treated rats in a manner similar to irradiated SM. In contrast, the mRNA levels of K(v) and K(ATP) were significantly increased while L-type calcium channels mRNA transcripts demonstrated tendency to increment. The SMCs obtained from irradiated animals and after KCNMA1 gene silencing showed a significant decrease in total K⁺ current density amplitude. Paxilline (500 nM)-sensitive components of outward current were significantly decreased in both irradiated and gene silencing SMCs. KCNMA1 gene silencing increased SM sensitivity to norepinephrine while Ach-induced relaxation had decreased. The silencing of KCNMA1 had no significant effect on BP while radiation produced sustained arterial hypertension. Therefore, radiation alters the form and function of the BK(Ca) channel and this type of channelopathy may contribute to related vascular abnormalities. Nevertheless, it is unlikely that BK(Ca) can operate as a crucial factor for radiation-induced arterial hypertension. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. Functional validation of Ca2+-binding residues from the crystal structure of the BK ion channel.

    Science.gov (United States)

    Kshatri, Aravind S; Gonzalez-Hernandez, Alberto J; Giraldez, Teresa

    2018-04-01

    BK channels are dually regulated by voltage and Ca 2+ , providing a cellular mechanism to couple electrical and chemical signalling. Intracellular Ca 2+ concentration is sensed by a large cytoplasmic region in the channel known as "gating ring", which is formed by four tandems of regulator of conductance for K + (RCK1 and RCK2) domains. The recent crystal structure of the full-length BK channel from Aplysia californica has provided new information about the residues involved in Ca 2+ coordination at the high-affinity binding sites located in the RCK1 and RCK2 domains, as well as their cooperativity. Some of these residues have not been previously studied in the human BK channel. In this work we have investigated, through site directed mutagenesis and electrophysiology, the effects of these residues on channel activation by voltage and Ca 2+ . Our results demonstrate that the side chains of two non-conserved residues proposed to coordinate Ca 2+ in the A. californica structure (G523 and E591) have no apparent functional role in the human BK Ca 2+ sensing mechanism. Consistent with the crystal structure, our data indicate that in the human channel the conserved residue R514 participates in Ca 2+ coordination in the RCK1 binding site. Additionally, this study provides functional evidence indicating that R514 also interacts with residues E902 and Y904 connected to the Ca 2+ binding site in RCK2. Interestingly, it has been proposed that this interaction may constitute a structural correlate underlying the cooperative interactions between the two high-affinity Ca 2+ binding sites regulating the Ca 2+ dependent gating of the BK channel. This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin McIlwain. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Efficacy of levofloxacin in the treatment of BK viremia: a multicenter, double-blinded, randomized, placebo-controlled trial.

    Science.gov (United States)

    Lee, Belinda T; Gabardi, Steven; Grafals, Monica; Hofmann, R Michael; Akalin, Enver; Aljanabi, Aws; Mandelbrot, Didier A; Adey, Deborah B; Heher, Eliot; Fan, Pang-Yen; Conte, Sarah; Dyer-Ward, Christine; Chandraker, Anil

    2014-03-01

    BK virus reactivation in kidney transplant recipients can lead to progressive allograft injury. Reduction of immunosuppression remains the cornerstone of treatment for active BK infection. Fluoroquinolone antibiotics are known to have in vitro antiviral properties, but the evidence for their use in patients with BK viremia is inconclusive. The objective of the study was to determine the efficacy of levofloxacin in the treatment of BK viremia. Enrollment in this prospective, multicenter, double-blinded, placebo-controlled trial occurred from July 2009 to March 2012. Thirty-nine kidney transplant recipients with BK viremia were randomly assigned to receive levofloxacin, 500 mg daily, or placebo for 30 days. Immunosuppression in all patients was adjusted on the basis of standard clinical practices at each institution. Plasma BK viral load and serum creatinine were measured monthly for 3 months and at 6 months. At the 3-month follow-up, the percentage reductions in BK viral load were 70.3% and 69.1% in the levofloxacin group and the placebo group, respectively (P=0.93). The percentage reductions in BK viral load were also equivalent at 1 month (58% versus and 67.1%; P=0.47) and 6 months (82.1% versus 90.5%; P=0.38). Linear regression analysis of serum creatinine versus time showed no difference in allograft function between the two study groups during the follow-up period. A 30-day course of levofloxacin does not significantly improve BK viral load reduction or allograft function when used in addition to overall reduction of immunosuppression.

  12. Sarcoma de Kaposi en un adulto con trasplante renal Kaposi's sarcoma in an adult with renal transplantation

    Directory of Open Access Journals (Sweden)

    Enrique Emilio Jiménez López

    2010-07-01

    Full Text Available Se presenta el caso clínico de un paciente con trasplante renal, atendido en el Hospital Provincial Docente "Saturnino Lora Torres" de Santiago de Cuba, que a los 12 meses de operado comenzó a presentar lesiones eritematosas en la piel. Los resultados de los exámenes complementarios, incluida la biopsia, confirmaron que se trataba de un sarcoma de Kaposi. El afectado egresó y continuó su seguimiento por consulta externa. A los 3 meses, la dermatopatía había desaparecido totalmente y disminuido a 50 % el índice de filtración glomerular.The case report of a patient with renal transplantion, attended in the «Saturnino Lora Torres» Teaching Provincial Hospital from Santiago de Cuba who, after 12 months of his surgery, began to present erythematous lesions in the skin is presented. The results of the additional tests, including the biopsy, confirmed that it was a Kaposi's sarcoma. He was discharged and continued his follow up through the out patient department. After 3 months, the dermatopathy had totally disappeared and the glomerular filtration index decreased to 50%.

  13. Ethanol modulation of mammalian BK channels in excitable tissues: molecular targets and their possible contribution to alcohol-induced altered behavior

    Directory of Open Access Journals (Sweden)

    Alex M. Dopico

    2014-12-01

    Full Text Available In most tissues, the function of calcium- and voltage-gated potassium (BK channels is modified in response to ethanol concentrations reached in human blood during alcohol intoxication. In general, modification of BK current from ethanol-naïve preparations in response to brief ethanol exposure results from changes in channel open probability without modification of unitary conductance or change in BK protein levels in the membrane. Protracted and/or repeated ethanol exposure, however, may evoke changes in BK expression. The final ethanol effect on BK open probability leading to either BK current potentiation or BK current reduction is determined by an orchestration of molecular factors, including levels of activating ligand (cytosolic calcium, BK subunit composition and posttranslational modifications, and the channel’s lipid microenvironment. These factors seem to allosterically regulate a direct interaction between ethanol and a recognition pocket of discrete dimensions recently mapped to the channel-forming (slo1 subunit. Type of ethanol exposure also plays a role in the final BK response to the drug: in several central nervous system regions (e.g., striatum, primary sensory neurons, and supraoptic nucleus, acute exposure to ethanol reduces neuronal excitability by enhancing BK activity. In contrast, protracted or repetitive ethanol administration may alter BK subunit composition and membrane expression, rendering the BK complex insensitive to further ethanol exposure. In neurohypophysial axon terminals, ethanol potentiation of BK channel activity leads to a reduction in neuropeptide release. In vascular smooth muscle, however, ethanol inhibition of BK current leads to cell contraction and vascular constriction.

  14. Urological management (medical and surgical of BK-virus associated haemorrhagic cystitis in children following haematopoietic stem cell transplantation

    Directory of Open Access Journals (Sweden)

    Nikhil Vasdev

    2013-10-01

    Full Text Available Aim: Haemorrhagic cystitis (HC is uncommon and in its severe form potentially life threatening complication of Haematopoietic stem cell transplantation (HSCT in children. We present our single centre experience in the urological management of this clinically challenging condition. Patients and Methods: Fourteen patients were diagnosed with BK-Virus HC in our centre. The mean age at diagnosis was 8.8 years (range, 3.2-18.4 years. The mean number of days post-BMT until onset of HC was 20.8 (range, 1 – 51. While all patients tested urine positive for BKV at the clinical onset of HC, only four patients had viral quantification, with viral loads ranging from 97,000 to >1 billion/ml. 8 patients had clinical HC. Ten patients experienced acute GVHD (grade I: 6 patients, grade II: 3 patients, grade 4: 1 patient.Results: Four patients received medical management for their HC. Treatments included hyperhydration, MESNA, blood and platelet transfusion, premarin and oxybutynin (Table 6.  Two patients received both medical and surgical management which included cystoscopy with clot evacuation, bladder irrigation and supra-pubic catheter insertion. One patient received exclusive surgical management. Seven patients were treated conservatively. Conclusion: There is limited available evidence for other potential therapeutic strategies highlighting the need for more research into the pathophysiology of HSCT-associated HC. Commonly used interventions with possible clinical benefit (e.g. cidofovir, ciprofloxacin still require to be evaluated in multi-centre, high-quality studies. Potential future preventative and therapeutic options, such as modulation of conditioning, immunosuppression and engraftment, new antiviral and anti-inflammatory and less nephrotoxic agents need to be assessed.---------------------------Cite this article as:Vasdev N, Davidson A, Harkensee C, Slatter M, Gennery A, Willetts I, Thorpe A.Urological management (medical and surgical of BK

  15. Impacto de la colonización respiratoria y de la bacteria asintomática sobre el riesgo de infección en candidatos a trasplante hepático y cardiaco

    OpenAIRE

    González Padilla, Marcelino

    2013-01-01

    Existen pocas descripciones de la epidemiología de la infección en pacientes candidatos a trasplante hepático1, y ninguna de pacientes candidatos a trasplante cardíaco. Varios autores han demostrado como el desarrollo de infecciones en pacientes con hepatopatía o cardiopatía avanzada empeoran el pronóstico y son causa de una buena parte de los ingresos hospitalarios de estos pacientes2,3. Existen sin embargo estudios que no encuentran una asociación entre el desarrollo de infecciones previame...

  16. Diagnóstico Precoz del Rechazo en el Trasplante Experimental de Páncreas Total con Duodeno en el Perro: Citología por Punción-Aspiración con Aguja Fina.

    OpenAIRE

    Rodríguez Abascal, Moisés

    2016-01-01

    El trasplante de páncreas está indicado fundamentalmente en los pacientes con diabetes mellitus tipo I. En la actualidad las soluciones terapéuticas ofertadas son: regímenes de insulina, bombas de infusión subcutánea de insulina, el páncreas artificial, y el trasplante de páncreas; este último sería la posibilidad que más fisiológicamente regularía el mecanismo de homostasis de la glucosa. Desde que...

  17. Analýza marketingové komunikace sportovního klubu BK Lokomotiva Karlovy Vary

    OpenAIRE

    Houdková, Jana

    2012-01-01

    ANALYSIS OF MARKETING COMMUNICATIONS BK LOKOMOTIVA KARLOVY VARY SPORTS CLUB Objectives: The aim of this work is to analyze the current state of marketing communication with the audience and sponsors for women's basketball club BK Lokomotiva Karlovy Vary and propose a strategy for improving marketing communications. Methods: In this thesis were performed using the methods of observation and personal interviews examined subjects Results: An analysis of marketing communication club, thanks to wh...

  18. BK Virus Load Associated with Serum Levels of sCD30 in Renal Transplant Recipients

    Science.gov (United States)

    Malik, Salma N.; Al-Saffer, Jinan M.; Jawad, Rana S.

    2016-01-01

    Background. Rejection is the main drawback facing the renal transplant operations. Complicated and overlapping factors, mainly related to the immune system, are responsible for this rejection. Elevated serum levels of sCD30 were frequently recorded as an indicator for renal allograft rejection, while BV virus is considered as one of the most serious consequences for immunosuppressive treatment of renal transplant recipients (RTRs). Aims. This study aimed to determine the association of BK virus load with serum levels of sCD30 in RTRs suffering from nephropathy. Patients and Methods. A total of 50 RTRs with nephropathy and 30 age-matched apparently healthy individuals were recruited for this study. Serum samples were obtained from each participant. Real-time PCR was used to quantify BK virus load in RTRs serum, while ELISA technique was employed to estimate serum levels of sCD30. Results. Twenty-two percent of RTRs had detectable BKV with mean viral load of 1.094E + 06 ± 2.291E + 06. RTRs showed higher mean serum level of sCD30 (20.669 ± 18.713 U/mL) than that of controls (5.517 ± 5.304 U/mL) with significant difference. BK virus load had significant positive correlation with the serum levels of sCD30 in RTRs group. Conclusion. These results suggest that serum levels of sCD30 could be used as an indicator of BK viremia, and accordingly the immunosuppressive regime should be adjusted. PMID:27051424

  19. Electronic structure and spectral properties of heavy actinides Pu, Am, Cm and Bk

    International Nuclear Information System (INIS)

    Shick, Alexander B; Kolorenc, Jindrich; Lichtenstein, Alexander I; Havela, Ladislav

    2010-01-01

    Selected electronic properties of Pu, Am, Cm and Bk are calculated with the aid of charge self-consistent LDA + Hubbard I method. Presented all-electron calculations are performed in the full-potential LAPW basis and incorporate spin-orbit interaction. The results are found to be in good agreement with experimental valence photoelectron spectra as well as with core XAS/EELS spectra of heavy actinides.

  20. BK channels regulate spontaneous action potential rhythmicity in the suprachiasmatic nucleus.

    Directory of Open Access Journals (Sweden)

    Jack Kent

    Full Text Available BACKGROUND: Circadian ( approximately 24 hr rhythms are generated by the central pacemaker localized to the suprachiasmatic nucleus (SCN of the hypothalamus. Although the basis for intrinsic rhythmicity is generally understood to rely on transcription factors encoded by "clock genes", less is known about the daily regulation of SCN neuronal activity patterns that communicate a circadian time signal to downstream behaviors and physiological systems. Action potentials in the SCN are necessary for the circadian timing of behavior, and individual SCN neurons modulate their spontaneous firing rate (SFR over the daily cycle, suggesting that the circadian patterning of neuronal activity is necessary for normal behavioral rhythm expression. The BK K(+ channel plays an important role in suppressing spontaneous firing at night in SCN neurons. Deletion of the Kcnma1 gene, encoding the BK channel, causes degradation of circadian behavioral and physiological rhythms. METHODOLOGY/PRINCIPAL FINDINGS: To test the hypothesis that loss of robust behavioral rhythmicity in Kcnma1(-/- mice is due to the disruption of SFR rhythms in the SCN, we used multi-electrode arrays to record extracellular action potentials from acute wild-type (WT and Kcnma1(-/- slices. Patterns of activity in the SCN were tracked simultaneously for up to 3 days, and the phase, period, and synchronization of SFR rhythms were examined. Loss of BK channels increased arrhythmicity but also altered the amplitude and period of rhythmic activity. Unexpectedly, Kcnma1(-/- SCNs showed increased variability in the timing of the daily SFR peak. CONCLUSIONS/SIGNIFICANCE: These results suggest that BK channels regulate multiple aspects of the circadian patterning of neuronal activity in the SCN. In addition, these data illustrate the characteristics of a disrupted SCN rhythm downstream of clock gene-mediated timekeeping and its relationship to behavioral rhythms.

  1. Chronic Prenatal Hypoxia Down-Regulated BK Channel Β1 Subunits in Mesenteric Artery Smooth Muscle Cells of the Offspring

    Directory of Open Access Journals (Sweden)

    Bailin Liu

    2018-02-01

    Full Text Available Background/Aims: Chronic hypoxia in utero could impair vascular functions in the offspring, underlying mechanisms are unclear. This study investigated functional alteration in large-conductance Ca2+-activated K+ (BK channels in offspring mesenteric arteries following prenatal hypoxia. Methods: Pregnant rats were exposed to normoxic control (21% O2, Con or hypoxic (10.5% O2, Hy conditions from gestational day 5 to 21, their 7-month-old adult male offspring were tested for blood pressure, vascular BK channel functions and expression using patch clamp and wire myograh technique, western blotting, and qRT-PCR. Results: Prenatal hypoxia increased pressor responses and vasoconstrictions to phenylephrine in the offspring. Whole-cell currents density of BK channels and amplitude of spontaneous transient outward currents (STOCs, not the frequency, were significantly reduced in Hy vascular myocytes. The sensitivity of BK channels to voltage, Ca2+, and tamoxifen were reduced in Hy myocytes, whereas the number of channels per patch and the single-channel conductance were unchanged. Prenatal hypoxia impaired NS1102- and tamoxifen-mediated relaxation in mesenteric arteries precontracted with phenylephrine in the presence of Nω-nitro-L-arginine methyl ester. The mRNA and protein expression of BK channel β1, not the α-subunit, was decreased in Hy mesenteric arteries. Conclusions: Impaired BK channel β1-subunits in vascular smooth muscle cells contributed to vascular dysfunction in the offspring exposed to prenatal hypoxia.

  2. Renovascular BK(Ca) channels are not activated in vivo under resting conditions and during agonist stimulation

    DEFF Research Database (Denmark)

    Magnusson, Linda; Sørensen, Charlotte Mehlin; Braunstein, Thomas Hartig

    2006-01-01

    We investigated the role of large-conductance Ca(2+)-activated K(+) (BK(Ca)) channels for the basal renal vascular tone in vivo. Furthermore, the possible buffering by BK(Ca) of the vasoconstriction elicited by angiotensin II (ANG II) or norepinephrine (NE) was investigated. The possible activation.......3 nmol/min) did not have any effect. Renal injection of ANG II (1-4 ng) or NE (10-40 ng) produced a transient decrease in RBF. These responses were not affected by preinfusion of TEA or IBT. Renal infusion of the BK(Ca) opener NS-1619 (90.0 nmol/min) did not affect basal RBF or the response to NE......, there is no indication for a major role for BK(Ca) channels in the control of basal renal tone in vivo. Furthermore, BK(Ca) channels do not have a buffering effect on the rat renal vascular responses to ANG II and NE. The fact that NS-1619 attenuates the ANG II response indicates that the renal vascular BK(Ca) channels...

  3. Conocimientos y actitudes de los médicos de instituciones hospitalarias en Barranquillla sobre donación y trasplante de órganos

    Directory of Open Access Journals (Sweden)

    Rafael Tuesca . M

    2003-01-01

    Full Text Available Objetivo: Identificar las actitudes y evaluar el conocimiento sobre donación y trasplante de órganos en médicos de instituciones hospitalarias en Barranquilla (Colombia. Métodos: Se diseñó un estudio observacional transversal. Muestra aleatoria estratificada de 305 médicos generales (MG y especialistas de instituciones hospitalarias de Barranquilla. Información de tipo primario, mediante encuestas autodiligenciadas. El cuestionario adaptado de Blanca, De Frutos y Rosel consta de 29 preguntas, divididas en tres capítulos. El primer capítulo: identificación personal (6 ítems; el segundo midió el conocimiento sobre donación y trasplante mediante preguntas de selección múltiple (11 ítems; y el tercero evaluó las actitudes con escala tipo likert (12 ítems. Se contó con el apoyo de los centros hospitalarios. Resultados: Se evaluaron 274 médicos (104 MG que presentaron una media de 48.7 años y desviación estándar de 6.37. La razón de médicos especialistas por médico general fue de 1.6:1. Con respectoalconocimientoteórico,seencontróun50.73%defrecuenciamodal derespuestasregulares y el 41.24% en el rango bueno. Al comparar el conocimiento con el área de trabajo se encontró una diferencia significativa respecto al grado de conocimiento entre médicos generales y especialistas, siendo (OR=2.37IC95%1.42–3.93.Losmédicosdesconocenelmarcolegalqueregulaladonación ytrasplantedeórganos.El72.3%delosencuestadosdesconocelaexistenciadelprogramadedonación y trasplante de órganos en Barranquilla. El 35% manifiestó que se actualiza al consultar libros o revistas especializadas. En cuanto al conocimiento general, existe diferencia significativa entre los médicos generales y especialistas, lo cual arrojó una prueba Z de diferencia de proporciones de 3.91 para una p= 0.00007. Conclusiones:Losinvestigadoressugierenentreotrosaspectos:promocionarydifundirlosprogramas de trasplante y donación de órganos para el público en general y

  4. Enfermedad Granulomatosa Crónica en paciente pediátrico y trasplante de médula ósea como tratamiento curativo

    OpenAIRE

    Constanza Martínez R.; Valentina Ulloa P.; Claudio Mosso C.

    2016-01-01

    Introducción: La enfermedad granulomatosa crónica (EGC) es una inmunodeficiencia primaria, caracterizada por la incapacidad de células fagocíticas para producir sustancias necesarias para destruir microorganismos. Actualmente, el trasplante de médula ósea como tratamiento curativo de la EGC ha demostrado ser una prometedora alternativa terapéutica. Presentación del caso: Lactante menor de 8 meses, ingresa a Unidad de Cuidados Especiales Pediátricos por cuadro de nueve días de evolución caract...

  5. Hemoterapia en el trasplante de médula ósea: Experiencia en el instituto de hematología e inmunología

    OpenAIRE

    Alfonso Valdés, María E; Bencomo Hernández, Antonio; Dorticós Balea, Elvira; Hernández Díaz, Patricia; Alfonso Valdés, Yalile; González Otero, Alejandro

    1999-01-01

    Se describe la hemoterapia aplicada a 19 pacientes con enfermedades hematológicas malignas que recibieron trasplante de médula ósea autólogo (n=10) y alogénico (n=9). El consumo promedio de concentrados de eritrocitos y concentrados de plaquetas fue de 6,1 y 57,3, respectivamente. El uso de ambos componentes fue mayor en los enfermos que recibieron una médula ósea alogénica. Los pacientes que recibieron tratamiento con ciclosporina requirieron un número menor de concentrados de eritrocitos y ...

  6. BK Virus-Associated Nephropathy: Current Situation in a Resource-Limited Country.

    Science.gov (United States)

    Yooprasert, P; Rotjanapan, P

    Data on BK virus-associated nephropathy (BKVAN) and treatment strategy in a resource-limited country are scarce. This study aimed to evaluate epidemiology of BKVAN and its situation in Thailand. A retrospective analysis was conducted among adult kidney transplant recipients at Ramathibodi Hospital from October 2011 to September 2016. Patients' demographic data, information on kidney transplantation, immunosuppressive therapy, cytomegalovirus and BK virus infections, and allograft outcomes were retrieved and analyzed. This study included 623 kidney transplant recipients. Only 327 patients (52.49%) received BK virus infection screening, and 176 of 327 patients had allograft dysfunction as a trigger for screening. BKVAN was identified in 39 of 327 patients (11.93%). Deceased donor transplantation and cytomegalovirus infection were associated with a higher risk of BKVAN (odds ratio = 2.2, P = .024, 95% confidence intervals [1.1, 4.43], and odds ratio = 2.6, P = .006, 95% confidence intervals [1.29, 5.26], respectively). BKVAN patients were at significantly higher risk for allograft rejection (P < .001) and allograft failure (P = .036). At the end of the study, 4 graft losses were documented (12.12%). BKVAN was associated with high rate of allograft rejection and failure. However, surveillance of its complications has been underperformed at our facility. Implementing a formal practice guideline may improve allograft outcome in resource-limited countries. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  7. A non-cardiomyocyte autonomous mechanism of cardioprotection involving the SLO1 BK channel

    Directory of Open Access Journals (Sweden)

    Andrew P. Wojtovich

    2013-03-01

    Full Text Available Opening of BK-type Ca2+ activated K+ channels protects the heart against ischemia-reperfusion (IR injury. However, the location of BK channels responsible for cardioprotection is debated. Herein we confirmed that openers of the SLO1 BK channel, NS1619 and NS11021, were protective in a mouse perfused heart model of IR injury. As anticipated, deletion of the Slo1 gene blocked this protection. However, in an isolated cardiomyocyte model of IR injury, protection by NS1619 and NS11021 was insensitive to Slo1 deletion. These data suggest that protection in intact hearts occurs by a non-cardiomyocyte autonomous, SLO1-dependent, mechanism. In this regard, an in-situ assay of intrinsic cardiac neuronal function (tachycardic response to nicotine revealed that NS1619 preserved cardiac neurons following IR injury. Furthermore, blockade of synaptic transmission by hexamethonium suppressed cardioprotection by NS1619 in intact hearts. These results suggest that opening SLO1 protects the heart during IR injury, via a mechanism that involves intrinsic cardiac neurons. Cardiac neuronal ion channels may be useful therapeutic targets for eliciting cardioprotection.

  8. BK virus has tropism for human salivary gland cells in vitro: Implications for transmission

    International Nuclear Information System (INIS)

    Jeffers, Liesl K.; Madden, Vicki; Webster-Cyriaque, Jennifer

    2009-01-01

    Background: In this study, it was determined that BKV is shed in saliva and an in vitro model system was developed whereby BKV can productively infect both submandibular (HSG) and parotid (HSY) salivary gland cell lines. Results: BKV was detected in oral fluids using quantitative real-time PCR (QRTPCR). BKV infection was determined using quantitative RT-PCR, immunofluorescence and immunoblotting assays. The infectivity of BKV was inhibited by pre-incubation of the virus with gangliosides that saturated the major capsid protein, VP1, halting receptor mediated BKV entry into salivary gland cells. Examination of infected cultures by transmission electron microscopy revealed 45-50 nm BK virions clearly visible within the cells. Subsequent to infection, encapsidated BK virus was detected in the supernatant. Conclusion: We thus demonstrated that BKV was detected in oral fluids and that BK infection and replication occur in vitro in salivary gland cells. These data collectively suggest the potential for BKV oral route of transmission and oral pathogenesis.

  9. Levels of 250Cf populated in the decay of 250Bk

    International Nuclear Information System (INIS)

    Uecke, J.W.

    1975-06-01

    The nuclide 250 Bk undergoes β-decay with a half-life of 3.2 h to 13.1 y 250 Cf. A study is undertaken of the excited states in 250 Cf populated by 250 Bk decay which results from the α-decay of 276d 254 Es. The general features of published level schemes for these nuclei are consistent with predictions of the Nilsson and collective models; however, there remain many undiscovered transitions and ambiguous or uncertain level assignments. In an attempt to confirm predictions of current theoretical models which account for nuclear level assignments in this nucleus, these gamma transitions and their levels have been studied. Twenty-eight new γ-rays were determined. The decay of 250 Bk is investigated primarily by high resolution gamma-ray singles spectrometry and supported in part by two-parameter gamma-gamma coincidence spectrometry. The equipment, comprised of a Ge(HP) and a large volume Ge(Li) detector, a 4096-channel two-parameter analyzer, and a PDP-8/e computer system, permitted significant improvement in sensitivity and accuracy over previous investigations on this nucleus. (11 figures, 2 tables) (U.S.)

  10. BK/TD models for analyzing in vitro impedance data on cytotoxicity.

    Science.gov (United States)

    Teng, S; Barcellini-Couget, S; Beaudouin, R; Brochot, C; Desousa, G; Rahmani, R; Pery, A R R

    2015-06-01

    The ban of animal testing has enhanced the development of new in vitro technologies for cosmetics safety assessment. Impedance metrics is one such technology which enables monitoring of cell viability in real time. However, analyzing real time data requires moving from static to dynamic toxicity assessment. In the present study, we built mechanistic biokinetic/toxicodynamic (BK/TD) models to analyze the time course of cell viability in cytotoxicity assay using impedance. These models account for the fate of the tested compounds during the assay. BK/TD models were applied to analyze HepaRG cell viability, after single (48 h) and repeated (4 weeks) exposures to three hepatotoxic compounds (coumarin, isoeugenol and benzophenone-2). The BK/TD models properly fit the data used for their calibration that was obtained for single or repeated exposure. Only for one out of the three compounds, the models calibrated with a single exposure were able to predict repeated exposure data. We therefore recommend the use of long-term exposure in vitro data in order to adequately account for chronic hepatotoxic effects. The models we propose here are capable of being coupled with human biokinetic models in order to relate dose exposure and human hepatotoxicity. Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  11. The temperature dependence of the BK channel activity - kinetics, thermodynamics, and long-range correlations.

    Science.gov (United States)

    Wawrzkiewicz-Jałowiecka, Agata; Dworakowska, Beata; Grzywna, Zbigniew J

    2017-10-01

    Large-conductance, voltage dependent, Ca 2+ -activated potassium channels (BK) are transmembrane proteins that regulate many biological processes by controlling potassium flow across cell membranes. Here, we investigate to what extent temperature (in the range of 17-37°C with ΔT=5°C step) is a regulating parameter of kinetic properties of the channel gating and memory effect in the series of dwell-time series of subsequent channel's states, at membrane depolarization and hyperpolarization. The obtained results indicate that temperature affects strongly the BK channels' gating, but, counterintuitively, it exerts no effect on the long-range correlations, as measured by the Hurst coefficient. Quantitative differences between dependencies of appropriate channel's characteristics on temperature are evident for different regimes of voltage. Examining the characteristics of BK channel activity as a function of temperature allows to estimate the net activation energy (E act ) and changes of thermodynamic parameters (ΔH, ΔS, ΔG) by channel opening. Larger E act corresponds to the channel activity at membrane hyperpolarization. The analysis of entropy and enthalpy changes of closed to open channel's transition suggest the entropy-driven nature of the increase of open state probability during voltage activation and supports the hypothesis about the voltage-dependent geometry of the channel vestibule. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Pabellón de trasplantes en el hospital general de Edimburgo, Gran Bretaña

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    Womersley, P.

    1970-11-01

    Full Text Available The set of buildings includes a medical wing, an administrative wing, and a slightly elevated passage, which connects the Nuffield Foundation with the Hospital, through which patients and donants are taken from the Hospital to the Pavillion. The body of the building, which is tower shaped, has stairs, a lift, water tanks; and in the semi-basement there are mechanical, sanitary, and air conditioning installations. This pavillion has been specially designed to carry out human transplants, mainly kidneys, and meets present complex technical needs in this branch of surgery. From the aesthetic aspect, the total designs gives the visitor the impression of safety and airiness, and induces a sensation of confidence.El conjunto construido consta de: un ala médica; un ala administrativa; y un paso ligeramente elevado, que une Nuffield con el Hospital, utilizado para el traslado de pacientes y donantes desde el Hospital al Pabellón. El cuerpo de edificio, con aspecto de «torre», contiene: la escalera, el ascensor, el depósito de agua, etc.; el semisótano alberga: los equipos mecánicos y los servicios de las instalaciones sanitarias, aire acondicionado, etc. Ha sido especialmente diseñado para realizar operaciones de trasplante de órganos humanos, en particular de riñon, y satisface adecuadamente las complejas exigencias técnicas que reclamaban los recientes avances en esta rama de la cirugía. Desde el punto de vista estético, el conjunto construido produce en el espectador una sensación segura y etérea, que lo hace sumamente atractivo.

  13. Educación en la promoción de estilos de vida saludables para la prevención de recaídas en pacientes con tuberculosis pulmonar bk (+), en el hospital Carlos Monge Medrano Juliaca 2010.

    OpenAIRE

    Maldonado Zanabria, Dionicia

    2011-01-01

    El presente estudio se realizó con el objetivo de evaluar la efectividad de la Educación en la Promoción de estilos de vida saludable para la prevención de recaídas en pacientes con Tuberculosis Pulmonar BK (+) en el Hospital Carlos Monge Medran o Juliaca 2010. El estudio es de carácter Cuasi-Experimental, con diseño de pre y post test; la muestra estuvo constituida por 18 pacientes. Para recolección de datos se utilizó como instrumentos un cuestionario. La prueba de hipótesis se realizó con ...

  14. Actitudes y conocimientos de Intensivistas colombianos sobre donación y trasplantes: Implicaciones para la nueva norma nacional de habilitación de servicios de salud.

    Directory of Open Access Journals (Sweden)

    David Andrés Castañeda Millán

    2015-01-01

    Full Text Available Introducción/Objetivo: Durante los últimos años Colombia se ha enfrentado a un descenso significativo en las tasas de donación de órganos, y por ende a una disminución en la cantidad de trasplantes realizados. En este contexto actualmente se implementa una nueva norma de habilitación para servicios de salud, que entre otras solicita al personal médico de cuidados intensivos capacitarse en gestión operativa de donación y trasplantes con el fin de optimizar la detección temprana y notificación de potenciales donantes; por lo tanto se pretende identificar las actitudes y conocimientos frente a la donación de órganos en médicos intensivistas de Colombia.  Materiales y Método: Estudio transversal descriptivo  realizado entre mayo y junio de 2013 a través de un formulario estructurado vía correo electrónico a los miembros de la Asociación Colombiana de Medicina Crítica y Cuidado Intensivo. Resultados: Se obtuvo un total de 48 participaciones efectivas. Del  total de participantes el 93,75% donaría sus órganos luego de morir, 97,91% donaría los órganos de un familiar fallecido, 95,83% donaría en vida uno de sus órganos para un familiar. El 83,33% ha manifestado a sus familiares la intención personal de donar; el 41,66% conoce alguna campaña de promoción sobre donación y el 12,5% tiene carné de donante. El 22,92% ha donado sangre en el último año; el 66,66% considera que se debe fortalecer la comunicación sobre donación en la unidad de cuidado intensivo, el 60,42% no aborda el tema de donación con  familias de pacientes neurocríticos con pobre pronóstico vital, el 54,17% no conoce la legislación colombiana sobre donación y trasplantes, el 18,75% califica como regular/mala la relación e interacción laboral con los coordinadores de trasplantes y el 43,75% tiene un concepto desfavorable sobre el modelo de trasplantes colombiano. Conclusiones: Los intensivistas colombianos tienen buena actitud  frente a la

  15. LA VISIÓN DEL PERSONAL DE SALUD SOBRE LA DONACIÓN Y EL TRASPLANTE DE ÓRGANOS: UNA REVISIÓN DE LA LITERATURA

    Directory of Open Access Journals (Sweden)

    Francisco Javier Mercado-Martínez

    2015-01-01

    Full Text Available El objetivo de este estudio fue revisar los estudios empíricos sobre la visión del personal de salud respecto a la donación y trasplante de órganos. Se hizo una revisión panorámica de la literatura en español, inglés y portugués; realizándose la búsqueda en dos buscadores y ocho bases de datos. El periodo abarca de 1985 al 2013. Se hizo análisis de contenido y frecuencias. De 316 artículos identificados, 248 fueron seleccionados. Según los hallazgos, los trabajos se concentraron en términos temporales y espaciales; predominando aquellos con metodologías cuantitativas. Las publicaciones priorizaron la perspectiva del personal médico y enfermería sobre las de otros actores, como directivos y coordinadores de donación. Las actitudes del personal sanitario hacia la donación de órganos de fallecido fue el tema más estudiado. Se concluye que una producción creciente de tipo cuantitativo analiza la perspectiva de médicos y enfermeras sobre la donación y trasplante de órganos de fallecido.

  16. 148. Utilidad del sistema oxigenador de membrana extracorpórea como puente al trasplante pulmonar y como asistencia quirúrgica para la realización del mismo. una nueva indicación en nuestro centro

    Directory of Open Access Journals (Sweden)

    J.A. Sarralde

    2012-04-01

    Conclusiones: La ECMO es útil para pacientes con insuficiencia respiratoria refractaria a medidas convencionales incluidos en lista de espera, así como para aquellos que precisen circulación extracorpórea para realización del trasplante pulmonar, disminuyendo las posibles complicaciones y aumentando la supervivencia.

  17. Reconstitución inmune exitosa mediante trasplante de células madre hematopoyéticas en un paciente colombiano afectado con enfermedad granulomatosa crónica

    Directory of Open Access Journals (Sweden)

    Yermis Carolina Rocha

    2016-06-01

    Conclusión. El trasplante de células madre hematopoyéticas permite la reconstitución completa de la función inmunitaria relacionada con la actividad microbicida de las células fagocíticas de pacientes con enfermedad granulomatosa crónica ligada al cromosoma X.

  18. Identification of pyrolysis products of the new psychoactive substance 2-amino-1-(4-bromo-2,5-dimethoxyphenyl)ethanone hydrochloride (bk-2C-B) and its iodo analogue bk-2C-I.

    Science.gov (United States)

    Texter, Kelly B; Waymach, Rachel; Kavanagh, Pierce V; O'Brien, John E; Talbot, Brian; Brandt, Simon D; Gardner, Elizabeth A

    2018-01-01

    2-Amino-1-(4-bromo-2,5-dimethoxyphenyl)ethanone hydrochloride (bk-2C-B) has recently emerged as a new psychoactive substance (NPS). It is most commonly consumed orally, although there are indications that it might also be ingested by inhalation or 'smoking'. Information about the stability of bk-2C-B when exposed to heat is unavailable and the potential for pyrolytic degradation and formation of unknown substances available for inhalation prompted an investigation using a simulated 'meth pipe' scenario. Twelve products following pyrolysis of bk-2C-B were detected and verified by organic synthesis of the corresponding standards. In addition, 2-amino-1-(4-iodo-2,5-dimethoxyphenyl)ethanone hydrochloride (bk-2C-I) was characterized for the first time and subjected to pyrolysis as well. Similar products were formed, which indicated that the replacement of the bromo with the iodo substituent did not affect the pyrolysis pattern under the conditions used. Two additional products were detected in the bk-2C-I pyrolates, namely 1-(2,5-dimethoxyphenyl)-ethanone and 1-iodo-4-ethenyl-5-methoxyphenol. The potential ingestion of pyrolysis products with unknown toxicity adds an element of concern. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  19. Educational intervention for liver transplantation candidates Intervención educativa para candidatos al trasplante de hígado Intervenção educativa para candidatos ao transplante de fígado

    Directory of Open Access Journals (Sweden)

    Karina Dal Sasso Mendes

    2013-02-01

    Full Text Available OBJECTIVE: The objective in this study was to analyze candidates' knowledge on the liver transplantation process before and after putting in practice an educational intervention. METHOD: A quasi-experimental, one-group pretest-posttest research design was adopted. The final sample included 15 subjects. Research data were collected between January and March 2010 in three phases, which were: pretest, implementation of the educational intervention (two meetings and posttest. RESULTS: The results evidenced significant cognitive gains after the intervention, with improvements in the participants' performance . CONCLUSIONS: The research presents evidence that putting in practice a patient education strategy can enhance candidates' knowledge on the liver transplantation process and consequently contribute to a successful treatment.OBJETIVO: El objetivo del estudio fue analizar el conocimiento de candidatos sobre el proceso de trasplante de hígado, antes y después de la implementación de una intervención educativa. MÉTODO: Se trata de un estudio con diseño casi experimental, del tipo grupo único, antes y después. La muestra final abarcó a 15 sujetos. La recolecta de datos de la investigación, con duración de enero a marzo del 2010, fue llevada a cabo en tres etapas, que son: pre-test, implementación de la intervención educativa (dos encuentros y post-test. RESULTADOS: Los resultados evidenciaron ganancia cognitiva significativa tras la intervención, con mejora en el desempeño de los participantes. CONCLUSIONES: El estudio evidencia que la implementación de una estrategia dirigida a la educación del paciente puede aumentar el conocimiento de candidatos sobre el proceso de trasplante de hígado y consecuentemente contribuir al éxito del tratamiento.OBJETIVO: o estudo teve como objetivo analisar o conhecimento de candidatos sobre o processo de transplante de fígado, antes e depois da implementação de intervenção educativa. M

  20. Comprehensive benchmarking reveals H2BK20 acetylation as a distinctive signature of cell-state-specific enhancers and promoters.

    Science.gov (United States)

    Kumar, Vibhor; Rayan, Nirmala Arul; Muratani, Masafumi; Lim, Stefan; Elanggovan, Bavani; Xin, Lixia; Lu, Tess; Makhija, Harshyaa; Poschmann, Jeremie; Lufkin, Thomas; Ng, Huck Hui; Prabhakar, Shyam

    2016-05-01

    Although over 35 different histone acetylation marks have been described, the overwhelming majority of regulatory genomics studies focus exclusively on H3K27ac and H3K9ac. In order to identify novel epigenomic traits of regulatory elements, we constructed a benchmark set of validated enhancers by performing 140 enhancer assays in human T cells. We tested 40 chromatin signatures on this unbiased enhancer set and identified H2BK20ac, a little-studied histone modification, as the most predictive mark of active enhancers. Notably, we detected a novel class of functionally distinct enhancers enriched in H2BK20ac but lacking H3K27ac, which was present in all examined cell lines and also in embryonic forebrain tissue. H2BK20ac was also unique in highlighting cell-type-specific promoters. In contrast, other acetylation marks were present in all active promoters, regardless of cell-type specificity. In stimulated microglial cells, H2BK20ac was more correlated with cell-state-specific expression changes than H3K27ac, with TGF-beta signaling decoupling the two acetylation marks at a subset of regulatory elements. In summary, our study reveals a previously unknown connection between histone acetylation and cell-type-specific gene regulation and indicates that H2BK20ac profiling can be used to uncover new dimensions of gene regulation. © 2016 Kumar et al.; Published by Cold Spring Harbor Laboratory Press.

  1. The RCK1 high-affinity Ca2+ sensor confers carbon monoxide sensitivity to Slo1 BK channels.

    Science.gov (United States)

    Hou, Shangwei; Xu, Rong; Heinemann, Stefan H; Hoshi, Toshinori

    2008-03-11

    Carbon monoxide (CO) is a lethal gas, but it is also increasingly recognized as a physiological signaling molecule capable of regulating a variety of proteins. Among them, large-conductance Ca(2+)- and voltage-gated K(+) (Slo1 BK) channels, important in vasodilation and neuronal firing, have been suggested to be directly stimulated by CO. However, the molecular mechanism of the stimulatory action of CO on the Slo1 BK channel has not been clearly elucidated. We report here that CO reliably and repeatedly activates Slo1 BK channels in excised membrane patches in the absence of Ca(2+) in a voltage-sensor-independent manner. The stimulatory action of CO on the Slo1 BK channel requires an aspartic acid and two histidine residues located in the cytoplasmic RCK1 domain, and the effect persists under the conditions known to inhibit the conventional interaction between CO and heme in other proteins. We propose that CO acts as a partial agonist for the high-affinity divalent cation sensor in the RCK1 domain of the Slo1 BK channel.

  2. Effect of temperature on surface error and laser damage threshold for self-healing BK7 glass.

    Science.gov (United States)

    Wang, Chu; Wang, Hongxiang; Shen, Lu; Hou, Jing; Xu, Qiao; Wang, Jian; Chen, Xianhua; Liu, Zhichao

    2018-03-20

    Cracks caused during the lapping and polishing process can decrease the laser-induced damage threshold (LIDT) of the BK7 glass optical elements, which would shorten the lifetime and limit the output power of the high-energy laser system. When BK7 glass is heated under appropriate conditions, the surface cracks can exhibit a self-healing phenomenon. In this paper, based on thermodynamics and viscous fluid mechanics theory, the mechanisms of crack self-healing are explained. The heat-healing experiment was carried out, and the effect of water was analyzed. The multi-spatial-frequency analysis was used to investigate the effect of temperature on surface error for self-healing BK7 glass, and the lapped BK7 glass specimens before and after heat healing were detected by an interferometer and atomic force microscopy. The low-spatial-frequency error was analyzed by peak to valley and root mean square, the mid-spatial-frequency error was analyzed by power spectral density, and the high-spatial-frequency error was analyzed by surface roughness. The results showed that the optimal heating temperature for BK7 was 450°C, and when the heating temperature was higher than the glass transition temperature (555°C), the surface quality decreased a lot. The laser damage test was performed, and the specimen heated at 450°C showed an improvement in LIDT.

  3. Effects of Nonlinear Absorption in BK7 and Color Glasses at 355 nm

    International Nuclear Information System (INIS)

    Adams, J J; McCarville, T; Bruere, J; McElroy, J; Peterson, J

    2003-01-01

    We have demonstrated a simple experimental technique that can be used to measure the nonlinear absorption coefficients in glasses. We determine BK7, UG1, and UG11 glasses to have linear absorption coefficients of 0.0217 ± 10% cm -1 , 1.7 ± 10% cm -1 , and 0.82 ± 10% cm -1 , respectively, two-photon absorption cross-sections of 0.025 ± 20% cm/GW, 0.035 ± 20% cm/GW, and 0.047 ± 20% cm/GW, respectively, excited-state absorption cross-sections of 8.0 x 10 -18 ± 20% cm 2 , 2.8 x 10 -16 ± 20% cm 2 , and 5 x 10 -17 ± 20% cm 2 , respectively, and solarization coefficients of 8.5 x 10 -20 ± 20% cm 2 , 2.5 x 10 -18 ± 20% cm 2 , and 1.3 x 10 -19 ± 20% cm 2 , respectively. For our application, nonlinear effects in 10-cm of BK7 are small ((le) 2%) for 355-nm fluences 2 for flat-top pulses. However, nonlinear effects are noticeable for 355-nm fluences at 0.8 J/cm 2 . In particular, we determine a 20% increase in the instantaneous absorption from linear, a solarization rate of 4% per 100 shots, and a 10% temporal droop introduced in the pulse, for 355-nm flat-top pulses at a fluence of 0.8 J/cm 2 . For 0.5-cm of UG1 absorbing glass the non-linear absorption has a similar effect as that from 10-cm of BK7 on the pulse shape; however, the effects in UG11 are much smaller

  4. Probing the Geometry of the Inner Vestibule of BK Channels with Sugars

    Science.gov (United States)

    Brelidze, Tinatin I.; Magleby, Karl L.

    2005-01-01

    The geometry of the inner vestibule of BK channels was probed by examining the effects of different sugars in the intracellular solution on single-channel current amplitude (unitary current). Glycerol, glucose, and sucrose decreased unitary current through BK channels in a concentration- and size-dependent manner, in the order sucrose > glucose > glycerol, with outward currents being reduced more than inward currents. The fractional decrease of outward current was more directly related to the fractional hydrodynamic volume occupied by the sugars than to changes in osmolality. For concentrations of sugars ≤1 M, the i/V plots for outward currents in the presence and absence of sugar superimposed after scaling, and increasing K+ i from 150 mM to 2 M increased the magnitudes of the i/V plots with little effect on the shape of the scaled curves. These observations suggest that sugars ≤1 M reduce outward currents mainly by entering the inner vestibule and reducing the movement of K+ through the vestibule, rather than by limiting diffusion-controlled access of K+ to the vestibule. With 2 M sucrose, the movement of K+ into the inner vestibule became diffusion limited for 150 mM K+ i and voltages >+100 mV. Increasing K+ i then relieved the diffusion limitation. An estimate of the capture radius based on the 5 pA diffusion-limited current for channels without the ring of negative charge at the entrance to the inner vestibule was 2.2 Å. Adding the radius of a hydrated K+ (6–8 Å) then gave an effective radius for the entrance to the inner vestibule of 8–10 Å. Such a functionally wide entrance to the inner vestibule together with our observation that even small concentrations of sugar in the inner vestibule reduce unitary current suggest that a wide inner vestibule is required for the large conductance of BK channels. PMID:16043773

  5. Characterization of an extracellular lipase by Pseudomonas koreensis BK-L07 isolated from soil.

    Science.gov (United States)

    Anbu, Periasamy

    2014-01-01

    Screening using spirit blue agar revealed that strain BK-L07 had the highest lipase activity. Furthermore, the isolated strain was identified as Pseudomonas sp. based on morphological, physiological, biochemical, and molecular analyses. The 16S rRNA gene sequence of strain BK-L07 shared a high similarity with that of Pseudomonas koreensis (99%). The nutritional conditions and physicochemical properties were influenced by P. koreensis BK-L07. The maximum lipase production was obtained in tryptic soy broth medium at pH 8.0 and a temperature of 25°C after 36 hr of incubation. In addition, the lipase activity was determined using different carbon sources and lipase inducers. The lipase production was greatest when 1% maltose was used as the carbon source and olive oil was used as the lipase inducer. The lipase production was significantly increased approximately threefold in the optimized medium when compared with the original medium. Further, the lipase was purified by ammonium sulfate precipitation and gel filtration chromatography with a purification yield of 10.8%. The molecular mass of lipase was 45 kDa. The optimum temperature and pH were 40°C and 8.0, respectively. The enzyme was stable up to 50°C and at pH from 7 to 9. In addition, the enzyme activity was stimulated by MgSO4 and completely inhibited by ethylenediamine tetraacetic acid (EDTA), indicating the metalloenzyme type. The lipase activity was toward medium to long chain length of fatty acids (C10 to C18). Supplemental materials are available for this article. Go to the publisher's online edition of Preparative Biochemistry and Biotechnology to view the supplemental file.

  6. Decay properties of Bk97243 and Bk97244

    Energy Technology Data Exchange (ETDEWEB)

    Ahmad, I.; Kondev, F. G.; Greene, J. P.; Zhu, S.

    2018-01-01

    Electron capture decays of Bk-243 and Bk-244 have been studied by measuring the gamma-ray spectra of mass-separated sources and level structures of Cm-243 and Cm-244 have been deduced. In Cm-243, the electron capture population to the ground state, 1/2(+)[631], and 1/2(+)[620] Nilsson states have been observed. The octupole K-pi = 2(-) band was identified in Cm-244 at 933.6 keV. In addition, spins and parities were deduced for several other states and two-quasiparticle configurations have been tentatively assigned to them

  7. Towards saturation of the electron-capture delayed fission probability: The new isotopes 240Es and 236Bk

    Directory of Open Access Journals (Sweden)

    J. Konki

    2017-01-01

    Full Text Available The new neutron-deficient nuclei 240Es and 236Bk were synthesised at the gas-filled recoil separator RITU. They were identified by their radioactive decay chains starting from 240Es produced in the fusion–evaporation reaction 209Bi(34S,3n240Es. Half-lives of 6(2s and 22−6+13s were obtained for 240Es and 236Bk, respectively. Two groups of α particles with energies Eα=8.19(3MeV and 8.09(3MeV were unambiguously assigned to 240Es. Electron-capture delayed fission branches with probabilities of 0.16(6 and 0.04(2 were measured for 240Es and 236Bk, respectively. These new data show a continuation of the exponential increase of ECDF probabilities in more neutron-deficient isotopes.

  8. Trasplante renal y disminución de la mortalidad en los programas de diálisis crónica

    Directory of Open Access Journals (Sweden)

    Walter G. Douthat

    2014-02-01

    Full Text Available El trasplante renal (TR presenta mejor supervivencia, calidad de vida y costos que la diálisis en la insuficiencia renal crónica (IRC. Estudiamos pacientes en diálisis que recibieron TR durante 2010, las causas de finalización del tratamiento y la supervivencia en diálisis. Evaluamos si criterios más amplios para la aceptación de trasplantes hubieran afectado los resultados del procedimiento en ese período. Incluimos 118 pacientes en diálisis, edad media 56.9 ± 18.4 años, tiempo en diálisis 45.5 ± 59.6 meses, 35 (30% presentaban diabetes como causa de IRC, y 58 (49% estaban en espera del TR. Treinta y cuatro finalizaron diálisis, 18 por TR y 12 por fallecimiento. Las principales causas de muerte fueron cardiovasculares, 6 (50% e infecciones, 2 (17%. La supervivencia al año fue 85% para el grupo total, 98% para los pacientes inscriptos en lista de espera y 72% para no inscriptos. Durante 2010 se realizaron 88 TR (62 con donantes cadavéricos [DC], 18 donantes vivos y 8 dobles trasplantes páncreas-riñón. Los receptores de DC tenían en promedio 50.7 años, 67 meses en diálisis, 8 (13% eran diabéticos, 12 (20% con TR previos y 3 cross match contra panel de anticuerpos > 20%. Los donantes tenían edad media 45 años, 28 (45% con criterios expandidos y 27.7 h de isquemia fría. A los 11.4 meses de seguimiento, 13 (21% presentó rechazo agudo, la supervivencia para injerto fue de 88% y 93% para pacientes. La principal causa de finalización de diálisis fue TR, sin detectarse que el empleo de DC afectara la supervivencia del TR.

  9. A single-center epidemiological study of BK virus infection and analysis of risk factors in patients with renal transplantation

    Directory of Open Access Journals (Sweden)

    Ji-gang LI

    2014-10-01

    Full Text Available Objective To investigate the epidemiological characteristics of BK virus (BKV infection in living renal transplantation patients, and analyze the risk factors of BKV infection and BKV nephropathy (BKVN. Methods The BKV DNA load in urine and blood samples of 43 renal transplant recipients, who had received renal transplantation in 309 Hospital from Feb. 2012 to Feb. 2013, was determined at preoperative period and 0.5, 1, 3, 6, 9, 12 and 15 months after transplantation. Meanwhile, the biopsy of grafted kidney was performed in those patients with continuously elevated serum creatinine and those with higher BKV DNA load. Patients were divided into 3 groups as follows according to the test results: BK viruria group, BK viremia group and pathologically diagnosed BKVN group. Data of each group were then recorded, including gender, age, postoperative diabetes (PTDM, acute rejection (AR, delayed recovery of graft function (DGF, postoperative pulmonary infection, preoperative immune induction therapy, postoperative immunosuppressive regimen, and other information. The risk factors for postoperative BKV infection and BKVN were analyzed. Results After an average of 15-month follow-up, it was found that the incidence of BKV viruria was 46.5%, that of BKV viremia was 14.0%, and that of BKVN was 2.3%. Sixth month after transplantation was found to be the peak time of viruria and viremia. FK506 was significantly associated with viremia in living donor renal transplantation. The immunosuppressive regimen was the immune related independent risk factor for BK viremia developing BKVN after living renal transplantation. Conclusion The incidence of BK viremia and BKVN is lower in living donor renal transplantation than in cadaver renal transplantation, but that of viruria is similar in both groups. Immunosuppressive scheme based on FK506 is an immune related independent risk factor leading to BK viremia proceeding to BKVN in living donor kidney

  10. Abnormal differentiation, hyperplasia and embryonic/perinatal lethality in BK5-T/t transgenic mice

    Science.gov (United States)

    Chen, Xin; Schneider-Broussard, Robin; Hollowell, Debra; McArthur, Mark; Jeter, Collene R.; Benavides, Fernando; DiGiovanni, John; Tang, Dean G.

    2009-01-01

    The cell-of-origin has a great impact on the types of tumors that develop and the stem/progenitor cells have long been considered main targets of malignant transformation. The SV40 large T and small t antigens (T/t), have been targeted to multiple differentiated cellular compartments in transgenic mice. In most of these studies, transgenic animals develop tumors without apparent defects in animal development. In this study, we used the bovine keratin 5 (BK5) promoter to target the T/t antigens to stem/progenitor cell-containing cytokeratin 5 (CK5) cellular compartment. A transgene construct, BK5-T/t, was made and microinjected into the male pronucleus of FVB/N mouse oocytes. After implanting ∼1700 embryos, only 7 transgenics were obtained, including 4 embryos (E9.5, E13, E15, and E20) and 3 postnatal animals, which died at P1, P2, and P18, respectively. Immunohistological analysis revealed aberrant differentiation and prominent hyperplasia in several transgenic CK5 tissues, especially the upper digestive organs (tongue, oral mucosa, esophagus, and forestomach) and epidermis, the latter of which also showed focal dysplasia. Altogether, these results indicate that constitutive expression of the T/t antigens in CK5 cellular compartment results in abnormal epithelial differentiation and leads to embryonic/perinatal animal lethality. PMID:19272531

  11. BK polyomavirus genotypes Ia and Ib1 exhibit different biological properties in renal transplant recipients.

    Science.gov (United States)

    Varella, Rafael B; Zalona, Ana Carolina J; Diaz, Nuria C; Zalis, Mariano G; Santoro-Lopes, Guilherme

    2018-01-02

    BK polyomavirus (BKV) is an opportunist agent associated with nephropathy (BKVAN) in 1-10% of kidney transplant recipients. BKV is classified into genotypes or subgroups according to minor nucleotidic variations with unknown biological implications. Studies assessing the possible association between genotypes and the risk of BKVAN in kidney transplant patients have presented conflicting results. In these studies, genotype Ia, which is highly prevalent in Brazil, was less frequently found and, thus, comparative data on the biological properties of this genotype are lacking. In this study, BKV Ia and Ib1 genotypes were compared according to their viral load, genetic evolution (VP1 and NCCR) - in a cohort of renal transplant recipients. The patients infected with Ia (13/23; 56.5%) genotype exhibited higher viral loads in urine [>1.4 log over Ib1 (10/23; 43.5%); p=0.025]. In addition, genotype Ia was associated with diverse mutations at VP1 loops and sites under positive selection outside loops, which were totally absent in Ib1. Although the number of viremic patients was similar, the three patients who had BK nephropathy (BKVAN) were infected with Ia genotype. NCCR architecture (ww or rr) were not distinctive between Ia and Ib1 genotypes. Ia genotype, which is rare in other published BKV cohorts, presented some diverse biological properties in transplanted recipients in comparison to Ib1. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Preliminary study on rotary ultrasonic machining of Bk-7 optical glass rod

    International Nuclear Information System (INIS)

    Hamzah, E.; Izman, S.; Khoo, C.Y.; Zainal Abidin, N.N.

    2007-01-01

    This paper presents an experimental observation on rotary ultrasonic machining (RUM) of BK7 optical glass rod. BK7 is a common technical optical glass for high quality optical components due to its high linear optical transmission in the visible range and is chemically stable. RUM is a hybrid machining process that combines the material removal mechanisms of diamond grinding and ultrasonic machining (USM) and it is non-thermal, non-chemical, creates no change in the microstructure, chemical or physical properties of the work piece. In the RUM, a controlled static load is applied to the rotating core drill with metal bonded diamond abrasive and is ultrasonically vibrated in the axial direction. A water-soluble coolant was used to cool the tool and sample during machining processes. By using DOE (Design of Experiment) approach, the effect of spindle speed and feed rate to the ultrasonic machinability had been developed. The main effects and two-factor interactions of process parameters (spindle speed) and feed rate) on output variables (MRR, surface roughness, opaqueness, chipping thickness and chipping size) are studied. (author)

  13. On the Operator ⨁Bk Related to Bessel Heat Equation

    Directory of Open Access Journals (Sweden)

    Wanchak Satsanit

    2010-01-01

    Full Text Available We study the equation (∂/∂tu(x,t=c2⊕Bku(x,t with the initial condition u(x,0=f(x for x∈Rn+. The operator ⊕Bk is the operator iterated k-times and is defined by ⊕Bk=((∑i=1pBxi4-(∑j=p+1p+qBxi4k, where p+q=n is the dimension of the Rn+, Bxi=∂2/∂xi2+(2vi/xi(∂/∂xi, 2vi=2αi+1, αi>-1/2, i=1,2,3,…,n, and k is a nonnegative integer, u(x,t is an unknown function for (x,t=(x1,x2,…,xn,t∈Rn+×(0,∞, f(x is a given generalized function, and c is a positive constant. We obtain the solution of such equation, which is related to the spectrum and the kernel, which is so called Bessel heat kernel. Moreover, such Bessel heat kernel has interesting properties and also related to the kernel of an extension of the heat equation.

  14. Experiência com transplante cardíaco heterotópico em pacientes com resistência pulmonar elevada: seguimento tardio Experiencia con trasplante cardíaco heterotópico en pacientes con resistencia pulmonar elevada: seguimiento tardío Experience with heterotopic heart transplantation in patients with elevated pulmonary vascular resistance: late follow-up

    Directory of Open Access Journals (Sweden)

    Jose Henrique Andrade Vila

    2010-02-01

    selecionados.FUNDAMENTO: En los últimos años el número de artículos sobre trasplante cardíaco heterotópico y, en particular, del seguimiento a largo plazo de estos pacientes, ha sido escaso en la literatura, inclusive internacional, lo que llevó al presente relato. OBJETIVO: Relatar la experiencia clínica inicial y la evolución tardía de cuatro pacientes sometidos a trasplante cardíaco heterotópico, su indicación y principales complicaciones. MÉTODOS: Las cirugías se realizaron entre 1992 y 2001, y la indicación de trasplante heterotópico, en todas, fue mediante RVP, variable de 4,8 UW; a 6.5 UW, con gradiente transpulmonar superior a 15 mmHg. En el tercer paciente, se realizó una anastomosis directa entre las arterias pulmonares sin empleo de tubo prostético, y, en el corazón nativo, se realizó una valvuloplastia mitral y aneurismectomía de VI. El esquema inmunosupresor inmediato fue doble, con ciclosporina y azatioprina en los tres primeros pacientes y ciclosporina y micofenolato mofetil en el cuarto paciente. RESULTADOS: Un óbito inmediato por falla del injerto, un óbito luego de dos años y medio por endocarditis en trombo intraventricular en el corazón nativo, y un tercer óbito seis años después del trasplante, por complicaciones postoperatorias de una cirugía en la válvula aórtica del corazón nativo. El restante, 15 años después del trasplante, se encuentra bien, en clase funcional II (NYHA, seis años después de una oclusión quirúrgica de la válvula aórtica del corazón nativo. CONCLUSIÓN: El trasplante cardíaco heterotópico es un procedimiento con resultado inferior al trasplante cardíaco ortotópico, por presentar mayor RVP. Los trombos intraventriculares en el corazón nativo, que exige anticoagulación prolongada, así como las complicaciones de válvula aórtica, también en el corazón nativo, pueden exigir tratamiento quirúrgico. Sin embargo, en un paciente, la sobrevida de 15 años mostró la eficacia a largo plazo de

  15. Perspectivas futuras de tratamiento en la insuficiencia cardíaca: del trasplante de células a la regeneración cardíaca

    OpenAIRE

    Prosper, F. (Felipe); Herreros, J. (Jesús); Barba, J. (Joaquín)

    2004-01-01

    En los últimos años hemos asistido a un interés creciente por el tratamiento de la insuficiencia cardíaca mediante el trasplante de células madre. Mientras que los estudios con células madre de músculo (mioblastos) se iniciaron hace más de 10 años, la posibilidad de que las células madre de la médula ósea tengan un enorme potencial de diferenciación y proliferación ha estimulado la investigación con otros tipos de células madre. Estos estudios experimentales han demost...

  16. Las células T reguladoras y su influencia en la sobrevida del trasplante renal Regulatory T cells and their influence in kidney allograft survival

    Directory of Open Access Journals (Sweden)

    Sonia Y. Velásquez

    2007-10-01

    Full Text Available La respuesta inmune desencadenada frente a un trasplante alogénico conduce usualmente a una respuesta efectora que resulta en el rechazo del aloinjerto; sin embargo, algunos individuos mantienen un trasplante funcionante a largo plazo sin signos de rechazo (tolerancia operacional, aun en ausencia de inmunosupresión. Se ha sugerido que los mismos mecanismos son responsables para la tolerancia hacia antígenos propios y aloantígenos. Uno de estos mecanismos es la regulación inmune y se han identificado varias subpoblaciones de células con propiedades reguladoras. Entre ellas, la población celular mejor caracterizada corresponde a las células T reguladoras (Tregs. Aunque las Tregs en ratones son CD4+CD25+, en humanos el fenotipo de las Treg está restringida a las células T CD4 con alta expresión de CD25 (CD25high y del factor de transcripción Foxp3. El análisis fenotípico y funcional de las células T reguladoras o supresoras circulantes en pacientes trasplantados tal vez sea útil para la detección de pacientes tolerantes operacionales. Además, una futura manipulación in vitro de estas células con fines terapéuticos podría conducir a lograr la inducción de tolerancia in vivo en el trasplante clínico. Aquí, revisamos la evidencia experimental y clínica del papel de las células reguladoras en la biología del trasplante.The immune response elicited by an allogenic transplant usually leads to an effector response resulting in allograft rejection; however, some individuals maintain a long-term functioning transplant without signs of rejection (operational tolerance even in the absence of immunosuppression. It has been suggested that the same mechanisms are responsible for tolerance to self-antigens and alloantigens. One of such mechanisms is immune regulation and several cell subsets with regulatory properties have been identified. Among them, the best characterized cell populations are the regulatory T cells (Treg. Although

  17. Supervivencia del injerto y del receptor en el trasplante renal en el hospital Guillermo Gran Benavente (Concepción, Chile

    Directory of Open Access Journals (Sweden)

    Magali Rodríguez Vidal

    2014-12-01

    Full Text Available Al ser el trasplante renal la mejor opción terapéutica para el tratamiento de la Insuficiencia Renal Terminal, en la ciudad de Concepción (Chile, se inició el programa de Trasplante Renal (TX en el año 1994. El objetivo de este trabajo es caracterizar la supervivencia del injerto renal de los TX realizados un hospital, de dicha ciudad mediante un estudio descriptivo, transversal, correlacional y comparativo de los resultados obtenidos en dos periodos, A: de 1994 a 2003 y, B: de 2004 a 2012. El análisis estadístico se realizó con el software SPSSv20.0. La funcion de supervivencia con la prueba no-paramétrica de Kaplan-Meier; y para comparaciones de variables se utilizó el test log-rank. De los resultados obtenidos cabe destacar que la supervivencia del paciente trasplantado en el período A fue mejor el primer al año (96%, pero fue menor a los 3 (90%, 5 (86% y 10 años (72% respecto al período B en que los valores fueron al año 95%, a los 3 el 90% a los 5 y 10 años el 87% y a los 10 años. La supervivencia del injerto también fue mayor en el periodo A que en el B, y mejor cuando hubo un tiempo de isquemia inferior a 24 horas y sin diferencias en relación al sexo del donante y del receptor y al tipo de tratamiento inmunosupreso. Las infeciones fueron la primera causa de muerte del paciente y las complicaciones quirúrgicas la principal causa de la pérdida del injerto.

  18. Structure of the (0+,1+) mesons Bs0 and Bs1, and the strong coupling constant gBs0BK and gBs1B*K

    International Nuclear Information System (INIS)

    Wang, Z. G.

    2008-01-01

    In this article, we take the point of view that the bottomed (0 + ,1 + ) mesons B s0 and B s1 are the conventional bs meson and calculate the strong coupling constants g B s0 BK and g B s1 B*K with the light-cone QCD sum rules. The numerical values of strong coupling constants g B s1 B*K and g B s0 BK are very large and support the hadronic dressing mechanism. Just like the scalar mesons f 0 (980), a 0 (980), D s0 and axial-vector meson D s1 , the (0 + ,1 + ) bottomed mesons B s0 and B s1 may have small bs kernels of the typical bs meson size. The strong couplings to the hadronic channels (or the virtual mesons loops) may result in smaller masses than the conventional bs mesons in the potential quark models and enrich the pure bs states with other components.

  19. Source Material and Concentration of Wildfire-Produced Pyrogenic Carbon Influence Post-Fire Soil Nutrient Dynamics

    Directory of Open Access Journals (Sweden)

    Lucas A. Michelotti

    2015-04-01

    Full Text Available Pyrogenic carbon (PyC is produced by the thermal decomposition of organic matter in the absence of oxygen (O. PyC affects nutrient availability, may enhance post-fire nitrogen (N mineralization rates, and can be a significant carbon (C pool in fire-prone ecosystems. Our objectives were to characterize PyC produced by wildfires and examine the influence that contrasting types of PyC have on C and N mineralization rates. We determined C, N, O, and hydrogen (H concentrations and atomic ratios of charred bark (BK, charred pine cones (PC, and charred woody debris (WD using elemental analysis. We also incubated soil amended with BK, PC, and WD at two concentrations for 60 days to measure C and N mineralization rates. PC had greater H/C and O/C ratios than BK and WD, suggesting that PC may have a lesser aromatic component than BK and WD. C and N mineralization rates decreased with increasing PyC concentrations, and control samples produced more CO2 than soils amended with PyC. Soils with PC produced greater CO2 and had lower N mineralization rates than soils with BK or WD. These results demonstrate that PyC type and concentration have potential to impact nutrient dynamics and C flux to the atmosphere in post-fire forest soils.

  20. PENGEMBANGAN INSTRUMEN ASESMEN KEBUTUHAN PERKEMBANGAN UNTUK PENYUSUNAN KURIKULUM DAN EVALUASI PROGRAM BK

    Directory of Open Access Journals (Sweden)

    Gendon Barus

    2013-01-01

    Full Text Available Penelitian pengembangan ini bertujuan menghasilkan instrumen asesmen kebutuhan peserta didik, disebut Invantori Kebutuhan Perkembangan Murid (IKPM. Penelitian mengikuti model Plomp (1999 dengan lima fase. Reliabilitas instrumen diestimasi dengan teknik Alpha Cronbach, validitas isi diperiksa oleh pakar dan telaah praktisi melalui FGD. Validitas empirik dan kecocokan model pengukuran dibuktikan dengan analisis faktor konfirmatori (CFA dengan program Lisrel 8.30. Telaah praktisi melibatkan 60 orang guru kelas V dan VI SD dalam dua tahap FGD, sedangkan uji coba empirik dan implementasi melibatkan murid kelas V dan VI. Hasil penelitian pengembang-an ini adalah: 1 IKPM dapat digunakan untuk menghimpun data kebutuhan perkembangan sebagai dasar penyusunan kurikulum bimbingan dan melaksanakan evaluasi program, bimbingan dan alat ukur evaluasi program bimbingan klasikal di sekolah dasar dan 2 teridentifikasi butir-butir kebutuhan per-kembangan murid yang intens dan sangat intens untuk dipenuhi melalui layanan bimbingan klasikal. Kata kunci: instrumen asesmen kebutuhan, kurikulum bimbingan, instrument evaluasi program BK ______________________________________________________________ DEVELOPMENT OF DEVELOPMENTAL NEEDS ASSESSMENT INSTRUMENT FOR CURRICULUM ARRANGEMENT AND EVALUATION OF BK PROGRAM Abstract The goal of this research is to produce a developmental needs assessment instrument, called Student’s Developmental Needs Inventory, which can be use to reveal the needs of students in elementary school. The research method follows the model of Research and Development (R & D by Plomp (1999 with five phases. The instrument which is developed in this study guidance and counseling (Bimbingan Konseling, BK in elementary school. Instrument reliability is estimated using by Alpha Cronbach technique, it content validity is checked by experts judgment and practitioner’s assessment with FGD technique. Furthermore, empirical validity and the goodness of fit

  1. Linfangioleiomiomatosis y trasplante pulmonar

    OpenAIRE

    Ansótegui Barrera, Emilio

    2012-01-01

    La linfangioleiomiomatosis (LAM) es una enfermedad rara que afecta predominantemente a la mujer, sobre todo en edad fértil. Se caracteriza por una proliferación anormal de células musculares lisas inmaduras, células LAM, que crecen de una manera aberrante en la vía aérea, parénquima, linfáticos y vasos sanguíneos pulmonares, lo que determina una evolución progresiva hacia la insuficiencia respiratoria que condiciona el fallecimiento de las pacientes. Tiene carácter multisistémico afectando as...

  2. Draft Genome Sequence of an Invasive Multidrug-Resistant Strain, Pseudomonas aeruginosa BK1, Isolated from a Keratitis Patient

    KAUST Repository

    Jeganathan, Lakshmi Priya

    2014-03-27

    Pseudomonas aeruginosa infections are difficult to treat due to the presence of a multitude of virulence factors and antibiotic resistance. Here, we report the draft genome sequence of P. aeruginosa BK1, an invasive and multidrug-resistant strain, isolated from a bacterial keratitis patient in southern India.

  3. Draft Genome Sequence of an Invasive Multidrug-Resistant Strain, Pseudomonas aeruginosa BK1, Isolated from a Keratitis Patient

    KAUST Repository

    Jeganathan, Lakshmi Priya; Prakash, Logambiga; Neelamegam, Sivakumar; Antony, Aju; Alqarawi, Sami; Prajna, Lalitha; Devarajan, Bharanidharan; Mohankumar, Vidyarani

    2014-01-01

    Pseudomonas aeruginosa infections are difficult to treat due to the presence of a multitude of virulence factors and antibiotic resistance. Here, we report the draft genome sequence of P. aeruginosa BK1, an invasive and multidrug-resistant strain

  4. Characterization of self-assembled virus-like particles of human polyomavirus BK generated by recombinant baculoviruses

    International Nuclear Information System (INIS)

    Li, T.-C.; Takeda, Naokazu; Kato, Kenzo; Nilsson, Josefina; Xing Li; Haag, Lars; Cheng, R. Holland; Miyamura, Tatsuo

    2003-01-01

    The major structural protein of the human polyomavirus BK (BKV), VP1, was expressed by using recombinant baculoviruses. A large amount of protein with a molecular mass of about 42 kDa was synthesized and identified by Western blotting. The protein was detected exclusively in the nuclei by immunofluorescent analysis and it was released into culture medium. The expressed BKV VP1 protein was self-assembled into virus-like particles (BK-VLPs) with two different sizes (50 and 26 nm in diameter), which migrated into four different bands in CsCl gradient with buoyant densities of 1.29, 1.30, 1.33, and 1.35 g/cm 3 . The immunological studies on the BK-VLPs suggested that they have similar antigenicity with those of authentic BKV particles. Cryoelectron microscopy and 3D image analysis further revealed that the larger BK-VLPs were composed of 72 capsomers which all were pentamers arranged in a T = 7 surface lattice. This system provides useful information for detailed studies of viral morphogenesis and the structural basis for the antigenicity of BKV

  5. Calidad de vida y estrategias de afrontamiento en pacientes con insuficiencia renal crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal

    Directory of Open Access Journals (Sweden)

    Paola Andrea Acosta Hernández

    2008-01-01

    Full Text Available Se comparó la calidad de vida y las estrategias de afrontamiento en 120 pacientes con Insuficiencia Renal Crónica sometidos a hemodiálisis, diálisis peritoneal o trasplante renal, teniendo en cuenta la edad, sexo, escolaridad, estado civil y ocupación. Los instrumentos utilizados fueron una ficha sociodemográfica, la prueba de calidad de vida WHOQOL-100 (Organización Mundial de la Salud, 1998 y el Cuestionario de Estrategias de Afrontamiento (Lazarus & Folkman, 1985. Se encontró que los pacientes en tratamiento de trasplante reportaban mayor calidad de vida y mejores estrategias de afrontamiento en comparación con los otros tratamientos, siendo mayoreslas puntuaciones en personas con edad de transición entre adultez media y tardía, mujeres, con estudios universitarios, casados y empleados. Se discuten las implicaciones de estos hallazgos.

  6. Estudio comparativo de dos soluciones de preservación en el trasplante hepático:la solución de Celsior y la solución de la Universidad de Wisconsin.

    OpenAIRE

    Deusa Gea, Saulo

    2009-01-01

    RESUMEN Las mejoras en las formulaciones de los líquidos de preservación han permitido un gran avance en los trasplantes de órganos. Aunque la solución de la Universidad de Wisconsin (SUW) es la solución intracelular más extensamente utilizada por los grupos de trasplante hepático, en los últimos años la aparición de la solución extracelular de Celsior® (SC), inicialmente diseñada como solución cardiopléjica, parece representar una alternativa válida a la SUW en la con...

  7. Diagnóstico precoz del rechazo en el trasplante experimental de páncreas total con duodeno en el perro: citología por punción-aspiración con aguja fina

    OpenAIRE

    Rodríguez Abascal, Moisés

    1993-01-01

    El trasplante de páncreas está indicado fundamentalmente en los pacientes con diabetes mellitus tipo I. En la actualidad las soluciones terapéuticas ofertadas son: regímenes de insulina, bombas de infusión subcutánea de insulina, el páncreas artificial, y el trasplante de páncreas; este último sería la posibilidad que más fisiológicamente regularía el mecanismo de homostasis de la glucosa. Desde que se descubrió la insulina hace ya más de 70 años, ha cambiado poco el tratamiento del enferm...

  8. Sarcoma de Kaposi por virus del herpes humano de tipo 8 en un receptor de trasplante hepático pediátrico: Caso clínico

    OpenAIRE

    Malla, Ivone; Pérez, Celeste; Cheang, Yu; Silva, Marcelo

    2013-01-01

    Los pacientes que reciben tratamiento inmunosupresor están en riesgo de desarrollar tumores malignos. La infección primaria o reactivación del virus del herpes humano de tipo 8 (HHV-8) puede predisponer al sarcoma de Kaposi después del trasplante de un órgano sólido. En los receptores de trasplantes pediátricos, este sarcoma tiene baja incidencia y mal pronóstico. Se informa la presentación clínica de un sarcoma de Kaposi en un ganglio linfático luego de una infección por HHV-8 en un niño a l...

  9. Hemorrhagic Cystitis due to BK Reactivation in a Young Female Treated for Hodgkin-Disease

    Directory of Open Access Journals (Sweden)

    R. Le Calloch

    2011-01-01

    Full Text Available Hodgkin's lymphoma is a disease with a high rate of curability under classic chemo-radiotherapy regimes. Complications due to chemotherapy could include viral reactivation due to chronic lymphopenia. BK virus (BKV is a polyoma virus belonging to the Papovaviridae family with antibody seroprevalences in healthy populations varying from 60% to 80%. Initial infections are asymptomatic usually occur in early childhood, after which the viruses remain latent in the kidneys or urothelium. Reactivation of BKV occurs in individuals with severe immunosuppression during HIV infections, transplantation or, exceptionally, after classical chemotherapy. BKV incidence is approximately 0% to 5% in immunocompetent individuals. Reactivation is associated with nephropathy and haemorrhagic cystitis. Herein, we present a case of a haemorrhagic cystitis due to BKV reactivation in a patient with Hodgkin's disease treated with chemotherapy.

  10. Detection of BK virus in urine from renal transplant subjects by mass spectrometry

    Directory of Open Access Journals (Sweden)

    Konietzny Rebecca

    2012-04-01

    Full Text Available Abstract Background The diagnosis and management of BK virus (BKV reactivation following renal transplantation continues to be a significant clinical problem. Following reactivation of latent virus, impaired cellular immunity enables sustained viral replication to occur in urothelial cells, which potentially leads to the development of BKV-associated nephropathy (BKVAN. Current guidelines recommend regular surveillance for BKV reactivation through the detection of infected urothelial cells in urine (decoy cells or viral nucleic acid in urine or blood. However, these methods have variable sensitivity and cannot routinely distinguish between different viral subtypes. We therefore asked whether mass spectrometry might be able to overcome these limitations and provide an additional non-invasive technique for the surveillance of BKV and identification of recipients at increased risk of BKVAN. Results Here we describe a mass spectrometry (MS-based method for the detection of BKV derived proteins directly isolated from clinical urine samples. Peptides detected by MS derived from Viral Protein 1 (VP1 allowed differentiation between subtypes I and IV. Using this approach, we observed an association between higher decoy cell numbers and the presence of the VP1 subtype Ib-2 in urine samples derived from a cohort of 20 renal transplant recipients, consistent with the hypothesis that certain viral subtypes may be associated with more severe BKVAN. Conclusions This is the first study to identify BK virus proteins in clinical samples by MS and that this approach makes it possible to distinguish between different viral subtypes. Further studies are required to establish whether this information could lead to stratification of patients at risk of BKVAN, facilitate distinction between BKVAN and acute rejection (AR, and ultimately improve patient treatment and outcomes.

  11. Uso de medicamentos inmunosupresores en pacientes con trasplante renal, hepático y de médula ósea, en una clínica de nivel III de Bogotá.

    Directory of Open Access Journals (Sweden)

    Jorge J. López G.

    2009-07-01

    Full Text Available Antecedentes. El trasplante de órganos en la actualidad es una opción terapéutica para los pacientes que cursan estadíos terminales de ciertas patologías; el uso de inmunosupresores contribuye en gran medida a la efectividad de este tipo de tratamientos, ya que es fundamental para evitar el rechazo. El no seguimiento del consumo de medicamentos nuevos, como es el caso de los inmunosupresores que suelen tener precios relativamente elevados, junto con la ampliación injustificada de indicaciones, puede ocasionar un aumento de los costos de la terapia farmacológica y, lo más importante, causar riesgos en el paciente trasplantado. Objetivos. Describir y caracterizar el uso de medicamentos inmunosupresores en pacientes con trasplante renal, de médula ósea alogénico y hepático. Material y métodos. Estudio observacional-descriptivo de corte transversal con recolección retrospectiva de la información a un año. Se trata de un estudio de utilización de medicamentos sobre hábitos de prescripción. Resultados. El desenlace global de la terapia del trasplante produce resultados satisfactorios en un 92,5 por ciento (49 pacientes, 5,7 por ciento (3 pacientes presentan rechazo con retiro del injerto, mientras que un paciente fallece después de ser sometido al trasplante. El medicamento más prescrito es el micofenolato de mofetil, con 41 prescripciones (28%, mientras que el más consumido es la prednisona con 2,38 dosis diaria definidas /100 pacientes/día. Conclusiones. Se evidencia la presencia de micofenolato, ciclosporina y corticoides en la mayoría de los esquemas con algunas prescripciones de anticuerpos monoclonales y globulina antitimocítica.

  12. Viviendo con trasplante renal, sin protección social en salud: ¿Qué dicen los enfermos sobre las dificultades económicas que enfrentan y sus efectos?

    Directory of Open Access Journals (Sweden)

    Francisco Javier Mercado-Martínez

    2014-10-01

    Full Text Available El trasplante es el mejor tratamiento para la insuficiencia renal, de acuerdo con la biomedicina; sin embargo, es una tecnología cara. Este artículo examina las dificultades económicas y sus efectos en personas con trasplante renal, pero sin protección social en salud. Para el estudio se desarrolló una investigación cualitativa en México. Participaron 21 pacientes trasplantados; se aplicaron entrevistas semiestructuradas, y se realizó un análisis de contenido. Los resultados muestran que las personas enfermas enfrentan dificultades económicas, debido a los costes de las terapias renales, particularmente, de las medicinas. Todo ello tiene efectos negativos: las personas con pocos recursos dejan el tratamiento con medicamentos, abandonan el protocolo, no asisten a las consultas médicas y disminuyen los gastos domésticos, incluyendo el de alimentación; además de suponer una merma en los ingresos familiares. En conclusión, el trasplante renal empobrece a las personas enfermas sin protección social en salud; es urgente la implementación de un sistema de protección social para esta población.

  13. Inclusión diferencial de extranjeros/migrantes a trasplantes de órganos: dilemas éticos frente a "prácticas no éticas"

    Directory of Open Access Journals (Sweden)

    Lourdes Basualdo

    Full Text Available Resumen Este artículo aborda el modo en que ciertos dilemas éticos vinculados al trasplante de órganos a extranjeros "no residentes", son movilizados por el Estado argentino en las normativas que definen un tratamiento diferenciado entre nacionales y no-nacionales en materia de trasplante, y cómo son puestos en relación con la política migratoria. Se muestra que existen mecanismos de inclusión diferencial que garantizan la inscripción a lista de espera sólo a nacionales y extranjeros "residentes" y permiten el trasplante con donante vivo a extranjeros "no residentes". Estos procesos de apertura y cierre de fronteras son posibles porque en el contexto actual coexisten la preocupación estatal en resguardar los bienes sociales proporcionados por el Estado (una de las funciones constitutivas del control migratorio y la obligación moral de los Estados-nación por el cuidado de la vida en el marco del gobierno humanitario.

  14. Crecimiento de Morus alba L. durante la etapa de establecimiento, a partir del trasplante de posturas Growth of Morus alba L. during the establishment stage, since seedling transplant

    Directory of Open Access Journals (Sweden)

    Gertrudis Pentón

    2012-06-01

    Full Text Available El objetivo de este estudio fue evaluar el comportamiento morfoagronómico de la especie Morus alba L. var.Tigreada durante la primera etapa de establecimiento, a partir del trasplante de posturas. Para ello, se determinó la respuesta del cultivo al manejo de estas para el trasplante, se caracterizó el crecimiento y se determinaron las variables lineales que permiten cuantificar el área foliar de dicha variedad. Las posturas se mantuvieron en el vivero durante 120 días y se trasplantaron en el período lluvioso. Los surcos se orientaron de este a oeste, con un marco de siembra de 1,0 x 0,5 m. Se mantuvo un adecuado control de las malezas, las plagas y las enfermedades. No se regó y se aplicó materia orgánica en el momento del trasplante. Se establecieron dos formas de manejo de las posturas para el trasplante: deshoje total, y corte a la altura de 50 cm y deshoje total. Durante la etapa inicial de crecimiento posterior al trasplante, se obtuvo un ligero aumento en la capacidad de rebrote de las posturas cortadas y deshojadas. Ello no afectó el establecimiento de la especie, pues a los 135 días la producción de biomasa foliar varió entre 100 y 116 g/planta. El crecimiento en los primeros 155 días describió una curva sigmoidea, caracterizada por un ritmo lento durante los primeros 21 días; a ello le siguió un crecimiento intenso entre los 30 y 135 días, el cual se tornó nuevamente lento con el inicio de la época seca o invernal. Se demostró que la medición del largo de la hoja y su ajuste a través de los modelos no lineales Y = B0+B1X+B2X2 y Y = B0+B1X+B2X2+B3X3 permiten estimar el área foliar, y ello se cumple para la condición de que el largo del óvalo foliar mida entre 1,3 y 20,4 cm. La alta correlación encontrada hace apropiado este procedimiento de estimación.The objective of this study was to evaluate the morphoagronomic performance of the specie Morus alba L. var. Tigreada during the first establishment stage

  15. Cooperación jurídica internacional en materia de donación y trasplante de organos: hacia un nuevo paradigma

    Directory of Open Access Journals (Sweden)

    Joaquín Cayón de las CUEVAS

    2013-04-01

    Full Text Available RESUMEN El trasplante de órganos puede contribuir decisivamente no sólo a salvar vidas sino también a mejorar la calidad de las mismas. Sin embargo, el problema fundamental al que se enfrenta este tipo de terapias es la desproporción entre la oferta y la demanda. El número de donantes es, por definición, muy limitado y la donación puede no resultar posible por motivos diversos tales como la falta de autorización familiar o la ausencia de condiciones necesarias. Así las cosas, la dificultad en el acceso a las terapias de trasplante constituye el reflejo de una de las desigualdades en salud. Por otra parte, la escasez de órganos hace pervivir el indeseable fenómeno de la comercialización internacional. Resulta evidente que el problema de la insuficiencia de donantes afecta a toda la comunidad trasplantadora internacional dado que las necesidades de trasplantes son equivalentes en las diferentes regiones del mundo. En este sentido, el futuro pasa por propiciar una progresiva integración de las políticas públicas en materia de donación y trasplantes de órganos, superando el actual estadio de cooperación intergubernamental, loable pero insuficiente, para adentrarnos gradualmente en un nuevo paradigma de coordinación internacional de ámbito regional, condicionado a la previa armonización de la legislación de las naciones afectadas, que permita cifras homologables de donantes. Este nuevo paradigma de coordinación debe instrumentar mecanismos de prevención del fenómeno del turismo de trasplantes pero también facilitar y potenciar el intercambio internacional de órganos, removiendo desigualdades en el acceso al tratamiento trasplantador. RESUMO O transplante de órgãos pode ser valiosa não só para salvar vidas, mas também para melhorar a qualidade dos pacientes. No entanto, o problema fundamental dessas terapias é o desequilíbrio entre oferta e demanda. O número de doadores é muito limitado e a doação pode não ser

  16. Impacto en un programa de trasplante renal de un protocolo de donación tras muerte cardiaca controlada con soporte de ECMO

    Directory of Open Access Journals (Sweden)

    José Luis Cobo Sánchez

    Full Text Available Resumen Objetivo: Analizar el impacto de un protocolo de donación tras muerte cardiaca controlada con soporte de sistema de oxigenación de membrana extracorpórea (PMCC-ECMO en el programa de trasplante renal de nuestro centro. Material y Método: Estudio retrospectivo, observacional, descriptivo en una cohorte de injertos renales (IR procedentes de un PMCC-ECMO. Se evalúan los IR procedentes de 8 pacientes con enfermedad irreversible (EI en los que se aplica limitación del esfuerzo terapéutico y se consideran como potenciales donantes durante el último semestre del 2014 y el primer trimestre de 2015. Se excluyeron los IR enviados fuera de la comunidad. Se evaluaron indicadores del proceso de donación (IPD y resultados clínicos de los injertos (RCI. Resultados: IPD: 100% hombres, edad media 60 años, tiempo medio agónico 9,37 minutos; causa EI 62,5% de origen pulmonar, 37,5% de origen neurológico. Se obtuvieron 13 riñones, 3 hígados y 2 pulmones: 2 IR fuera de la comunidad. Los IR provenientes del PMCC-ECMO supusieron un 20% de los IR del 2014 en nuestro centro. RCI: De los 11 IR trasplantados en nuestro centro, sólo 1 no fue viable por trombosis de las venas renales, y otro IR se retrasó la función renal por causas inmunológicas. Valores medios función IR al mes: creatinina sérica 1,88 mg/dl, aclaramiento creatinina 56,82 mL/min, urea 0,798 g/L. Conclusiónes: El PMCC-ECMO ha tenido un alto impacto en el programa de trasplante renal, tanto por incrementar la tasa de donación, como por la alta tasa de viabilidad de los injertos.

  17. Evaluating the impact of pre-transplant desensitization utilizing a plasmapheresis and low-dose intravenous immunoglobulin protocol on BK viremia in renal transplant recipients.

    Science.gov (United States)

    Gabardi, S; Townsend, K; Martin, S T; Chandraker, A

    2013-08-01

    A correlation exists between polyomavirus BK (BKV) viremia in renal transplant recipients (RTR) and the degree of immunosuppression. However, the impact of pre-transplant desensitization on the incidence of BKV viremia is unknown. This retrospective study evaluated living-donor RTR between January 2004 and December 2008 receiving routine BKV viral load monitoring. Patients were divided into those who underwent pre-transplant desensitization (n = 20) and those who did not (n = 71). The primary endpoint was the incidence of BKV viremia at 1 year post transplant. All demographic data were similar, except for more female patients (65% vs. 36.6%; P = 0.0392) in the desensitized group. More desensitized patients had a previous transplant (75% vs. 12.7%; P < 0.0001) and were more likely to be induced with basiliximab (75% vs. 35.2%; P = 0.0021). Following transplantation, antibody-mediated rejection (AMR) rates were highest in the desensitized group (55% vs. 1.4%; P < 0.0001). The incidence of BKV viremia at 1 year post transplant was significantly higher in desensitized patients (45% vs. 19.7%; P = 0.0385). Desensitization was also associated with a higher prevalence of BKV viremia at any time post transplant (50% vs. 22.5%; P = 0.0245), polyomavirus-associated nephropathy (20% vs. 2.8%; P = 0.0198) and BKV-related allograft loss (10% vs. 0%; P = 0.0464). Also of note, in a subgroup analysis of only our desensitized patients, it did not appear that development of AMR significantly impacted the incidence of BKV viremia in these individuals. This analysis reveals that pre-transplant desensitization significantly increases the risk for BKV viremia and nephropathy. © 2013 John Wiley & Sons A/S.

  18. Dwarf Elliptical Galaxies in the M81 Group: The Structure and Stellar Populations of BK5N and F8D1

    OpenAIRE

    Caldwell, Nelson; Armandroff, Taft E.; Da Costa, G. S.; Seitzer, Patrick

    1997-01-01

    We have obtained HST WFPC2 images through the F555W and F814W filters of two M81 group dE's: BK5N and a new system, designated F8D1. The resulting color-magnitude diagrams show the upper two magnitudes of the red giant branch. Surface brightness and total magnitude measurements indicate that BK5N and F8D1 have similar central surface brightness (24.5 and 25.4 mag/arcsec^2 in V, respectively), but F8D1's larger length scale results in it being 3 magnitudes more luminous than BK5N. BK5N lies on...

  19. Mārketinga komunikācijas pielietojums atjaunotā BK "VEF Rīga" zīmola virzīšanā

    OpenAIRE

    Līcis, Mārtiņš

    2014-01-01

    Darba tēma ir „Mārketinga komunikācijas pielietojums atjaunotā BK „VEF Rīga” zīmola virzīšanā”. Darba mērķis ir noskaidrot mārketinga komunikācijas pielietojumu atjaunotā BK „VEF Rīga” zīmola virzīšanā. Uzdevumi ir izstudēt nepieciešamo teoriju, izanalizēt mārketinga komunikācijas instrumentu pielietojumu, noskaidrot BK „VEF Rīga”, sporta mārketinga speciālista un sabiedrības viedokli par BK „VEF Rīga” zīmolu. Teoriju veido: zīmols, zīmolvedība, mārketinga komunikācija, sociālo mediju mārketi...

  20. Chemical consequences of radioactive decay. 1. Study of 249Cf ingrowth into crystalline 249BkBr3: a new crystalline phase of CfBr3

    International Nuclear Information System (INIS)

    Young, J.P.; Haire, R.G.; Peterson, J.R.; Ensor, D.D.; Fellows, R.L.

    1980-01-01

    Spectrophotometric and x-ray powder diffraction methods have been applied to a study of the ingrowth of californium-249 by β - decay of berkelium-249 in crystalline 249 BkBr 3 . It was found that the Cf daughter grows in with the same oxidation state and crystal structure as the parent. Thus, six-coordinate BkBr 3 (AlCl 3 -type monoclinic structure) generates six-coordinate CfBr 3 , and eight-coordinate BkBr 3 (PuBr 3 -type orthorhombic structure) generates eight-coordinate CfBr 3 , a previously unknown form of CfBr 3 . It was also found that the daughter Cf(III) in the BkBr 3 parent compound can be reduced to Cf(II) by treatment with H 2 , as it can in pure CfBr 3 . 5 figures

  1. Differential Regulation of Action Potential Shape and Burst-Frequency Firing by BK and Kv2 Channels in Substantia Nigra Dopaminergic Neurons.

    Science.gov (United States)

    Kimm, Tilia; Khaliq, Zayd M; Bean, Bruce P

    2015-12-16

    Little is known about the voltage-dependent potassium currents underlying spike repolarization in midbrain dopaminergic neurons. Studying mouse substantia nigra pars compacta dopaminergic neurons both in brain slice and after acute dissociation, we found that BK calcium-activated potassium channels and Kv2 channels both make major contributions to the depolarization-activated potassium current. Inhibiting Kv2 or BK channels had very different effects on spike shape and evoked firing. Inhibiting Kv2 channels increased spike width and decreased the afterhyperpolarization, as expected for loss of an action potential-activated potassium conductance. BK inhibition also increased spike width but paradoxically increased the afterhyperpolarization. Kv2 channel inhibition steeply increased the slope of the frequency-current (f-I) relationship, whereas BK channel inhibition had little effect on the f-I slope or decreased it, sometimes resulting in slowed firing. Action potential clamp experiments showed that both BK and Kv2 current flow during spike repolarization but with very different kinetics, with Kv2 current activating later and deactivating more slowly. Further experiments revealed that inhibiting either BK or Kv2 alone leads to recruitment of additional current through the other channel type during the action potential as a consequence of changes in spike shape. Enhancement of slowly deactivating Kv2 current can account for the increased afterhyperpolarization produced by BK inhibition and likely underlies the very different effects on the f-I relationship. The cross-regulation of BK and Kv2 activation illustrates that the functional role of a channel cannot be defined in isolation but depends critically on the context of the other conductances in the cell. This work shows that BK calcium-activated potassium channels and Kv2 voltage-activated potassium channels both regulate action potentials in dopamine neurons of the substantia nigra pars compacta. Although both

  2. Eritrocitosis post trasplante renal y su tratamiento farmacológico: inhibidores de la enzima convertidora de angiotensina versus bloqueadores de los receptores de angiotensina en un caso

    OpenAIRE

    Duclos H,Jaime

    2000-01-01

    A 35 years old male is presented. Eight months after receiving a renal allograft his hypertension worsened and his packed red cell volume raised to 53.7%. Enalapril was started and a 500 ml phlebotomy was performed. In three occasions packed red cell volumes decreased excessively and enalapril was discontinued. Finally, the drug was replaced with losartan, normalizing blood pressure values and stabilizing packed red cell volumes. This case illustrates the different reductions in packed red ce...

  3. Inhibition of CUG-binding protein 1 and activation of caspases are critically involved in piperazine derivative BK10007S induced apoptosis in hepatocellular carcinoma cells.

    Directory of Open Access Journals (Sweden)

    Ju-Ha Kim

    Full Text Available Though piperazine derivative BK10007S was known to induce apoptosis in pancreatic cancer xenograft model as a T-type CaV3.1 a1G isoform calcium channel blocker, its underlying antitumor mechanism still remains unclear so far. Thus, in the present study, the antitumor mechanism of BK10007S was elucidated in hepatocellular carcinoma cells (HCCs. Herein, BK10007S showed significant cytotoxicity by 3-[4,5-2-yl]-2,5-diphenyltetra-zolium bromide (MTT assay and anti-proliferative effects by colony formation assay in HepG2 and SK-Hep1 cells. Also, apoptotic bodies and terminal deoxynucleotidyl transferase (TdT dUTP Nick End Labeling (TUNEL positive cells were observed in BK10007S treated HepG2 and SK-Hep1 cells by 4',6-diamidino-2-phenylinodole (DAPI staining and TUNEL assay, respectively. Consistently, BK10007S increased sub G1 population in HepG2 and SK-Hep1 cells by cell cycle analysis. Furthermore, Western blotting revealed that BK10007S activated the caspase cascades (caspase 8, 9 and 3, cleaved poly (ADP-ribose polymerase (PARP, and downregulated the expression of cyclin D1, survivin and for CUG-binding protein 1 (CUGBP1 or CELF1 in HepG2 and SK-Hep1 cells. Conversely, overexpression of CUGBP1 reduced cleavages of PARP and caspase 3, cytotoxicity and subG1 population in BK10007S treated HepG2 cells. Overall, these findings provide scientific evidences that BK10007S induces apoptosis via inhibition of CUGBP1 and activation of caspases in hepatocellular carcinomas as a potent anticancer candidate.

  4. Properties of optical breakdown in BK7 glass induced by an extended-cavity femtosecond laser oscillator.

    Science.gov (United States)

    Do, Binh T; Phillips, Mark C; Miller, Paul A; Kimmel, Mark W; Britsch, Justin; Cho, Seong-Ho

    2009-02-16

    Using an extended-cavity femtosecond oscillator, we investigated optical breakdown in BK7 glass caused by the accumulated action of many laser pulses. By using a pump-probe experiment and collecting the transmitted pump along with the reflected pump and the broadband light generated by the optical breakdown, we measured the build-up time to optical breakdown as a function of the pulse energy, and we also observed the instability of the plasma due to the effect of defocusing and shielding created by the electron gas. The spectrum of the broadband light emitted by the optical breakdown and the origin of the material modification in BK7 glass was studied. We developed a simple model of electromagnetic wave propagation in plasma that is consistent with the observed behavior of the reflection, absorption, and transmission of the laser light.

  5. Double-Nanodomain Coupling of Calcium Channels, Ryanodine Receptors, and BK Channels Controls the Generation of Burst Firing.

    Science.gov (United States)

    Irie, Tomohiko; Trussell, Laurence O

    2017-11-15

    Action potentials clustered into high-frequency bursts play distinct roles in neural computations. However, little is known about ionic currents that control the duration and probability of these bursts. We found that, in cartwheel inhibitory interneurons of the dorsal cochlear nucleus, the likelihood of bursts and the interval between their spikelets were controlled by Ca 2+ acting across two nanodomains, one between plasma membrane P/Q Ca 2+ channels and endoplasmic reticulum (ER) ryanodine receptors and another between ryanodine receptors and large-conductance, voltage- and Ca 2+ -activated K + (BK) channels. Each spike triggered Ca 2+ -induced Ca 2+ release (CICR) from the ER immediately beneath somatic, but not axonal or dendritic, plasma membrane. Moreover, immunolabeling demonstrated close apposition of ryanodine receptors and BK channels. Double-nanodomain coupling between somatic plasma membrane and hypolemmal ER cisterns provides a unique mechanism for rapid control of action potentials on the millisecond timescale. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Study of the properties of the superheavy nuclei Z=117 produced in the 249Bk + 48Ca reaction

    International Nuclear Information System (INIS)

    Oganessian, Y.T.; Abdullin, F.S.; Dmitriev, S.N.; Itkis, M.G.; Polyakov, A.N.; Sagaidak, R.N.; Shirokovsky, I.V.; Shumeiko, M.V.; Subbotin, V.G.; Sukhov, A.M.; Tsyganov, Y.S.; Utyonkov, V.K.; Voinov, A.A.; Vostokin, G.K.; Alexander, C.; Binder, J.; Boll, R.A.; Ezold, J.; Felker, K.; Miernik, K.; Roberto, J.B.; Rykaczewski, K.P.; Gostic, J.M.; Henderson, R.A.; Moody, K.J.; Shaughnessy, D.H.; Stoyer, M.A.; Stoyer, N.J.; Grzywacz, R.K.; Miller, D.; Hamilton, J.H.; Ramayya, A.V.; Ryabinin, M.A.

    2014-01-01

    The reaction of 249 Bk with 48 Ca have been reinvestigated to provide new evidence for the discovery of element 117 on a larger number of events. The experiments were performed at five projectile energies and with a total beam dose of 48 Ca of about 4.6*10 19 . Two isotopes 293,294 117 were synthesized in the 249 Bk+ 48 Ca reaction, providing excitation functions and α-decay spectra of the produced isotopes that establishes these nuclei to be the products of the 4n- and 3n-evaporation channels, respectively. Decay properties of 293,294 117 and of all the daughter products agree with the data of the experiment in which these nuclei were synthesized for the first time in 2010. The new 289 115 events, populated by a decay of 293 117, demonstrate the same decay properties as those observed for 289 115 produced in the 243 Am( 48 Ca,2n) reaction thus providing cross-bombardment evidence. In addition, a single decay of 294 118 was observed from the reaction with 249 Cf - a result of the in-growth of 249 Cf in the 249 Bk target. (authors)

  7. Dynamics of Db isotopes formed in reactions induced by 238U, 248Cm, and 249Bk across the Coulomb barrier

    Science.gov (United States)

    Kaur, Gurjit; Sandhu, Kirandeep; Kaur, Amandeep; Sharma, Manoj K.

    2018-05-01

    The dynamical cluster decay model is employed to investigate the decay of *265Db and *267Db nuclei, formed in the 27Al+238U , 18O+249Bk , and 19F+248Cm hot fusion reactions at energies around the Coulomb barrier. First, the fission dynamics of the 27Al+238U reaction is explored by investigating the fragmentation and preformation yield of the reaction. The symmetric mass distribution of the fission fragments is observed for *265Db nucleus, when static β2 i deformations are used within hot optimum orientation approach. However, the mass split gets broaden for the use of β2 i-dynamical hot configuration of the fragments and becomes clearly asymmetric for the cold-static-deformed approach. Within the application of cold orientations of fragments, a new fission channel is observed at mass asymmetry η =0.29 . In addition to 238U-induced reaction, the work is carried out to address the fission and neutron evaporation cross sections of *267Db nucleus formed via 19F+248Cm and 18O+249Bk reactions, besides a comprehensive analysis of fusion and capture processes. Higher fusion cross sections and compound nucleus formation probabilities (PCN) are obtained for the 18O+249Bk reaction, as larger mass asymmetry in the entrance channel leads to reduced Coulomb factor. Finally, the role of sticking (IS) and nonsticking (INS) moments of inertia is analyzed for the 4 n and 5 n channels of *267Db nuclear system.

  8. Trasplante de Médula Ósea 10 Años de Experiencia en la Clínica de Marly”.

    Directory of Open Access Journals (Sweden)

    Enrique Pedraza Mesa

    2004-03-01

    El desarrollo del programa necesitó implementar muchísimos aspectos tanto científicos como de enfermería, protocolos, mejorar el banco de sangre y el laboratorio clínico, la farmacia, ya que el trasplante utiliza una gran cantidad de medicamentos sumamente sofisticados y costosos que inicialmente tuvimos que importar directamente, tuvimos que mejorar el área física, hacer un laboratorio de criopreservación. Las células que se utilizan se obtienen de la sangre periférica de los propios pacientes que van a ser trasplantados o de un donante, en ese caso de un hermano idéntico, y estas células se llevan al laboratorio, se mezclan con un medio de cultivo y con un agente criopreservante y se congelan en nitrógeno líquido; por eso es necesario utilizar unas máquinas que llevan estas células a una temperatura de -170ºC...

  9. Varicela y herpes zóster en una paciente con trasplante renal Chickenpox and herpes zoster in a patient with kidney transplant

    Directory of Open Access Journals (Sweden)

    Enrique Emilio Jiménez López

    2011-03-01

    Full Text Available Se describe el caso clínico de una adolescente de 13 años de edad, con trasplante renal, que reingresó a los 10 meses de operada por presentar fiebre, dolor en el costado izquierdo desde el borde esternal de ese lado hasta la columna dorsal y lesiones maculopapulosas sobre una base eritematosa, localizadas en tronco, cuello y cara, que se extendieron primeramente a los miembros inferiores y superiores; pero luego, mientras unas se convirtieron en pústulas y costras típicas de un herpes zóster, las restantes evolucionaron como una varicela. Tratada oportunamente, la paciente mejoró su estado general y egresó con función renal normal a su entorno cotidiano.The clinical report of a 13 years-old adolescent with kidney transplant is described. He was readmitted ten months after the surgery due to fever, pain in the left side from the sternal margin to the thoracic spine and macular-papular injuries over erythematous base, located in the trunk, neck and face that were first spread to the lower and upper limbs, but then, while ones became into pustules and scales typical of herpes zoster, the remaining progressed as chickenpox. Treated at the appropriate time, the patient improved her general condition and she was discharged with normal kidney function to her daily environment.

  10. Trasplante de mucosa oral en la reconstrucción de las vías lagrimales Oral mucosa transplants in restoration of lacrimal canaliculi

    Directory of Open Access Journals (Sweden)

    Bernardo Canto Vidal

    2000-06-01

    Full Text Available Se realizaron 143 intervenciones de las vías lagrimales, mediante la técnica quirúrgica de trasplante de mucosa oral con 55 injertos libres y 88 tubulares, a partir del año 1995. Para ello se tuvo en cuenta el examen clínico y radiológico y el tipo de afectación. Con la utilización de las técnicas de injerto libre o tubular de la mucosa se puede restablecer la fisiología lagrimal en los pacientes con mutilaciones por traumatismos o presencia de tumores con disminución del riesgo quirúrgico; además se recomienda por novedosa, práctica y por los buenos resultados obtenidos.143 interventions of lacrimal canaliculi were carried out, throgh surgical technique of oral mucosa transplant (55 free grafts and 88 tutular ones since 1995, bearing in min radiological and clinical examination and type of invelvement. Using technique of free or tubular graft, it is possible restore lacrimal physiology in patients with mutilations from trauma or prevence of tumors thus decreasing surgical risk; this tachnique is recommended by its novelty, usefulness . and by good results obtained.

  11. Cistitis hemorrágica relacionada al trasplante de médula ósea: primer reporte de casos en el país

    Directory of Open Access Journals (Sweden)

    Juan Nunura

    2004-06-01

    Full Text Available Objetivo: Determinar la incidencia, características clínicas y complicaciones de la cistitis hemorrágica (CH. Material y Métodos: Estudio descriptivo sobre pacientes transplantados de médula ósea en la Unidad de Trasplante de Médula Ósea del Hospital Nacional Edgardo Rebagliati Martins, desde noviembre 1994 hasta octubre 2003, y que desarrollaron cistitis hemorrágica. Resultados: En 9 años fueron transplantados 170 pacientes, desarrollando cistitis hemorrágica 8 de ellos (incidencia acumulada 4,7%. El cuadro clínico se caracterizó por disuria más hematuria macroscópica o microscópica. La presentación tardía fue la más frecuente. En 4 pacientes, la cistitis hemorrágica fue severa. Dos de los tres pacientes que desarrollaron el cuadro de cistitis hemorrágica en dos oportunidades, fallecieron. Conclusiones: La mitad de los pocos pacientes que desarrollan CH en la Unidad, lo hacen bajo la forma severa y, si presentan la CH por segunda vez, su pronóstico se complica.

  12. BK virus encephalopathy and sclerosing vasculopathy in a patient with hypohidrotic ectodermal dysplasia and immunodeficiency.

    Science.gov (United States)

    Darbinyan, Armine; Major, Eugene O; Morgello, Susan; Holland, Steven; Ryschkewitsch, Caroline; Monaco, Maria Chiara; Naidich, Thomas P; Bederson, Joshua; Malaczynska, Joanna; Ye, Fei; Gordon, Ronald; Cunningham-Rundles, Charlotte; Fowkes, Mary; Tsankova, Nadejda M

    2016-07-13

    Human BK polyomavirus (BKV) is reactivated under conditions of immunosuppression leading most commonly to nephropathy or cystitis; its tropism for the brain is rare and poorly understood. We present a unique case of BKV-associated encephalopathy in a man with hypohidrotic ectodermal dysplasia and immunodeficiency (HED-ID) due to IKK-gamma (NEMO) mutation, who developed progressive neurological symptoms. Brain biopsy demonstrated polyomavirus infection of gray and white matter, with predominant involvement of cortex and distinct neuronal tropism, in addition to limited demyelination and oligodendroglial inclusions. Immunohistochemistry demonstrated polyoma T-antigen in neurons and glia, but expression of VP1 capsid protein only in glia. PCR analysis on both brain biopsy tissue and cerebrospinal fluid detected high levels of BKV DNA. Sequencing studies further identified novel BKV variant and disclosed unique rearrangements in the noncoding control region of the viral DNA (BKVN NCCR). Neuropathological analysis also demonstrated an unusual form of obliterative fibrosing vasculopathy in the subcortical white matter with abnormal lysosomal accumulations, possibly related to the patient's underlying ectodermal dysplasia. Our report provides the first neuropathological description of HED-ID due to NEMO mutation, and expands the diversity of neurological presentations of BKV infection in brain, underscoring the importance of its consideration in immunodeficient patients with unexplained encephalopathy. We also document novel BKVN NCCR rearrangements that may be associated with the unique neuronal tropism in this patient.

  13. UV protection filters by dielectric multilayer thin films on Glass BK-7 and Infrasil 301

    International Nuclear Information System (INIS)

    Abdel-Aziz, M.M.; Azim, Osama A.; Abdel-Wahab, L.A.; Seddik, Mohamed M.

    2006-01-01

    The increasing use of Ultraviolet (UV) light in medicine, industrial environments, for cosmetic use, and even in consumer products necessitates that greater attention be paid to the potential hazards of this type of electromagnetic radiation. To avoid any adverse effects of exposure to this type of radiation, four suitable protection filters were produced to block three UV bands (UVA, UVB, and UVC). The design structure of the required dielectric multilayer filters was done by optical thin film technology using the absorbing property of UV radiation for the substrates and dielectric materials. The computer analyses of the multilayer filter formulas were prepared using Macleod Software for the production processes. The deposition technique was achieved on optical substrates (Glass BK-7 and Infrasil 301) by dielectric material combinations including Titanium dioxide (Ti 2 O 3 ), Hafnium dioxide (HfO 2 ), and Lima (mixture of oxides SiO 2 /Al 2 O 3 ); deposition being achieved using an electron beam gun. The output results of the theoretical and experimental transmittance values for spectral band from 200 nm to 800 nm were discussed in four processes. To analyze the suitability for use in 'real world' applications, the test pieces were subjected to the durability tests (adhesion, abrasion resistance, and humidity) according to Military Standard MIL-C-675C and MIL-C-48497A

  14. UV protection filters by dielectric multilayer thin films on Glass BK-7 and Infrasil 301

    Science.gov (United States)

    Abdel-Aziz, M. M.; Azim, Osama A.; Abdel-Wahab, L. A.; Seddik, Mohamed M.

    2006-10-01

    The increasing use of Ultraviolet (UV) light in medicine, industrial environments, for cosmetic use, and even in consumer products necessitates that greater attention be paid to the potential hazards of this type of electromagnetic radiation. To avoid any adverse effects of exposure to this type of radiation, four suitable protection filters were produced to block three UV bands (UVA, UVB, and UVC). The design structure of the required dielectric multilayer filters was done by optical thin film technology using the absorbing property of UV radiation for the substrates and dielectric materials. The computer analyses of the multilayer filter formulas were prepared using Macleod Software for the production processes. The deposition technique was achieved on optical substrates (Glass BK-7 and Infrasil 301) by dielectric material combinations including Titanium dioxide (Ti 2O 3), Hafnium dioxide (HfO 2), and Lima (mixture of oxides SiO 2/Al 2O 3); deposition being achieved using an electron beam gun. The output results of the theoretical and experimental transmittance values for spectral band from 200 nm to 800 nm were discussed in four processes. To analyze the suitability for use in 'real world' applications, the test pieces were subjected to the durability tests (adhesion, abrasion resistance, and humidity) according to Military Standard MIL-C-675C and MIL-C-48497A.

  15. Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor

    Science.gov (United States)

    Su, Li-Chen; Tian, Ya-Chung; Chang, Ying-Feng; Chou, Chien; Lai, Chao-Sung

    2014-01-01

    In renal transplant patients, immunosuppressive therapy may result in the reactivation of polyomavirus BK (BKV), leading to polyomavirus-associated nephropathy (PVAN), which inevitably causes allograft failure. Since the treatment outcomes of PVAN remain unsatisfactory, early identification and continuous monitoring of BKV reactivation and reduction of immunosuppressants are essential to prevent PVAN development. The present study demonstrated that the developed dual-channel heterodyne-based surface plasmon resonance (SPR) biosensor is applicable for the rapid detection of urinary BKV. The use of a symmetrical reference channel integrated with the poly(ethylene glycol)-based low-fouling self-assembled monolayer to reduce the environmental variations and the nonspecific noise was proven to enhance the sensitivity in urinary BKV detection. Experimentally, the detection limit of the biosensor for BKV detection was estimated to be around 8500 copies/mL. In addition, urine samples from five renal transplant patients were tested to rapidly distinguish PVAN-positive and PVAN-negative renal transplant patients. By virtue of its simplicity, rapidity, and applicability, the SPR biosensor is a remarkable potential to be used for continuous clinical monitoring of BKV reactivation.

  16. P-waves imaging of the FRI and BK zones at the Grimsel Rock Laboratory

    International Nuclear Information System (INIS)

    Majer, E.L.; Peterson, J.E. Jr.; Blueming, P.; Sattel, G.

    1990-08-01

    This report is one of a series documenting the results of the Nagra-DOE Cooperative (NDC-I) research program in which the cooperating scientists explore the geological, geophysical, hydrological, geochemical, and structural effects anticipated from the use of a rock mass as a geological repository for nuclear waste. Tomographic imaging studies using a high frequency (10 Khz.) piezoelectric source and a three component receiver were carried out in two different regions of the underground Nagra Grimsel test facility in Switzerland. Both sites were in fractured granite, one being in a strongly foliated granite (FRI site), and the other being in a relatively homogeneous granite (BK zone). The object of the work was to determine if the seismic techniques could be useful in imaging the fracture zones and provide information on the hydrologic conditions. Both amplitude and velocity tomograms were obtained from the Data. The results indicate that the fracture zones strongly influenced the seismic wave propagation, thus imaging the fracture zones that were hydrologically important. 11 refs., 24 figs

  17. Human BK Polyomavirus—The Potential for Head and Neck Malignancy and Disease

    Directory of Open Access Journals (Sweden)

    Raquel Burger-Calderon

    2015-07-01

    Full Text Available Members of the human Polyomaviridae family are ubiquitous and pathogenic among immune-compromised individuals. While only Merkel cell polyomavirus (MCPyV has conclusively been linked to human cancer, all members of the polyomavirus (PyV family encode the oncoprotein T antigen and may be potentially carcinogenic. Studies focusing on PyV pathogenesis in humans have become more abundant as the number of PyV family members and the list of associated diseases has expanded. BK polyomavirus (BKPyV in particular has emerged as a new opportunistic pathogen among HIV positive individuals, carrying harmful implications. Increasing evidence links BKPyV to HIV-associated salivary gland disease (HIVSGD. HIVSGD is associated with elevated risk of lymphoma formation and its prevalence has increased among HIV/AIDS patients. Determining the relationship between BKPyV, disease and tumorigenesis among immunosuppressed individuals is necessary and will allow for expanding effective anti-viral treatment and prevention options in the future.

  18. Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus▿

    Science.gov (United States)

    Bista, Bipin Raj; Ishwad, Chandra; Wadowsky, Robert M.; Manna, Pradip; Randhawa, Parmjeet Singh; Gupta, Gaurav; Adhikari, Meena; Tyagi, Rakhi; Gasper, Gina; Vats, Abhay

    2007-01-01

    Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for “point of care” screening of BKV. PMID:17314224

  19. Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus.

    Science.gov (United States)

    Bista, Bipin Raj; Ishwad, Chandra; Wadowsky, Robert M; Manna, Pradip; Randhawa, Parmjeet Singh; Gupta, Gaurav; Adhikari, Meena; Tyagi, Rakhi; Gasper, Gina; Vats, Abhay

    2007-05-01

    Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for "point of care" screening of BKV.

  20. Overexpression of the Large-Conductance, Ca2+-Activated K+ (BK) Channel Shortens Action Potential Duration in HL-1 Cardiomyocytes.

    Science.gov (United States)

    Stimers, Joseph R; Song, Li; Rusch, Nancy J; Rhee, Sung W

    2015-01-01

    Long QT syndrome is characterized by a prolongation of the interval between the Q wave and the T wave on the electrocardiogram. This abnormality reflects a prolongation of the ventricular action potential caused by a number of genetic mutations or a variety of drugs. Since effective treatments are unavailable, we explored the possibility of using cardiac expression of the large-conductance, Ca2+-activated K+ (BK) channel to shorten action potential duration (APD). We hypothesized that expression of the pore-forming α subunit of human BK channels (hBKα) in HL-1 cells would shorten action potential duration in this mouse atrial cell line. Expression of hBKα had minimal effects on expression levels of other ion channels with the exception of a small but significant reduction in Kv11.1. Patch-clamped hBKα expressing HL-1 cells exhibited an outward voltage- and Ca2+-sensitive K+ current, which was inhibited by the BK channel blocker iberiotoxin (100 nM). This BK current phenotype was not detected in untransfected HL-1 cells or in HL-1 null cells sham-transfected with an empty vector. Importantly, APD in hBKα-expressing HL-1 cells averaged 14.3 ± 2.8 ms (n = 10), which represented a 53% reduction in APD compared to HL-1 null cells lacking BKα expression. APD in the latter cells averaged 31.0 ± 5.1 ms (n = 13). The shortened APD in hBKα-expressing cells was restored to normal duration by 100 nM iberiotoxin, suggesting that a repolarizing K+ current attributed to BK channels accounted for action potential shortening. These findings provide initial proof-of-concept that the introduction of hBKα channels into a cardiac cell line can shorten APD, and raise the possibility that gene-based interventions to increase hBKα channels in cardiac cells may hold promise as a therapeutic strategy for long QT syndrome.

  1. Differential T cell response against BK virus regulatory and structural antigens: A viral dynamics modelling approach.

    Directory of Open Access Journals (Sweden)

    Arturo Blazquez-Navarro

    2018-05-01

    Full Text Available BK virus (BKV associated nephropathy affects 1-10% of kidney transplant recipients, leading to graft failure in about 50% of cases. Immune responses against different BKV antigens have been shown to have a prognostic value for disease development. Data currently suggest that the structural antigens and regulatory antigens of BKV might each trigger a different mode of action of the immune response. To study the influence of different modes of action of the cellular immune response on BKV clearance dynamics, we have analysed the kinetics of BKV plasma load and anti-BKV T cell response (Elispot in six patients with BKV associated nephropathy using ODE modelling. The results show that only a small number of hypotheses on the mode of action are compatible with the empirical data. The hypothesis with the highest empirical support is that structural antigens trigger blocking of virus production from infected cells, whereas regulatory antigens trigger an acceleration of death of infected cells. These differential modes of action could be important for our understanding of BKV resolution, as according to the hypothesis, only regulatory antigens would trigger a fast and continuous clearance of the viral load. Other hypotheses showed a lower degree of empirical support, but could potentially explain the clearing mechanisms of individual patients. Our results highlight the heterogeneity of the dynamics, including the delay between immune response against structural versus regulatory antigens, and its relevance for BKV clearance. Our modelling approach is the first that studies the process of BKV clearance by bringing together viral and immune kinetics and can provide a framework for personalised hypotheses generation on the interrelations between cellular immunity and viral dynamics.

  2. Analysis of real-time mixture cytotoxicity data following repeated exposure using BK/TD models.

    Science.gov (United States)

    Teng, S; Tebby, C; Barcellini-Couget, S; De Sousa, G; Brochot, C; Rahmani, R; Pery, A R R

    2016-08-15

    Cosmetic products generally consist of multiple ingredients. Thus, cosmetic risk assessment has to deal with mixture toxicity on a long-term scale which means it has to be assessed in the context of repeated exposure. Given that animal testing has been banned for cosmetics risk assessment, in vitro assays allowing long-term repeated exposure and adapted for in vitro - in vivo extrapolation need to be developed. However, most in vitro tests only assess short-term effects and consider static endpoints which hinder extrapolation to realistic human exposure scenarios where concentration in target organs is varies over time. Thanks to impedance metrics, real-time cell viability monitoring for repeated exposure has become possible. We recently constructed biokinetic/toxicodynamic models (BK/TD) to analyze such data (Teng et al., 2015) for three hepatotoxic cosmetic ingredients: coumarin, isoeugenol and benzophenone-2. In the present study, we aim to apply these models to analyze the dynamics of mixture impedance data using the concepts of concentration addition and independent action. Metabolic interactions between the mixture components were investigated, characterized and implemented in the models, as they impacted the actual cellular exposure. Indeed, cellular metabolism following mixture exposure induced a quick disappearance of the compounds from the exposure system. We showed that isoeugenol substantially decreased the metabolism of benzophenone-2, reducing the disappearance of this compound and enhancing its in vitro toxicity. Apart from this metabolic interaction, no mixtures showed any interaction, and all binary mixtures were successfully modeled by at least one model based on exposure to the individual compounds. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. Analysis of real-time mixture cytotoxicity data following repeated exposure using BK/TD models

    International Nuclear Information System (INIS)

    Teng, S.; Tebby, C.; Barcellini-Couget, S.; De Sousa, G.; Brochot, C.; Rahmani, R.; Pery, A.R.R.

    2016-01-01

    Cosmetic products generally consist of multiple ingredients. Thus, cosmetic risk assessment has to deal with mixture toxicity on a long-term scale which means it has to be assessed in the context of repeated exposure. Given that animal testing has been banned for cosmetics risk assessment, in vitro assays allowing long-term repeated exposure and adapted for in vitro – in vivo extrapolation need to be developed. However, most in vitro tests only assess short-term effects and consider static endpoints which hinder extrapolation to realistic human exposure scenarios where concentration in target organs is varies over time. Thanks to impedance metrics, real-time cell viability monitoring for repeated exposure has become possible. We recently constructed biokinetic/toxicodynamic models (BK/TD) to analyze such data (Teng et al., 2015) for three hepatotoxic cosmetic ingredients: coumarin, isoeugenol and benzophenone-2. In the present study, we aim to apply these models to analyze the dynamics of mixture impedance data using the concepts of concentration addition and independent action. Metabolic interactions between the mixture components were investigated, characterized and implemented in the models, as they impacted the actual cellular exposure. Indeed, cellular metabolism following mixture exposure induced a quick disappearance of the compounds from the exposure system. We showed that isoeugenol substantially decreased the metabolism of benzophenone-2, reducing the disappearance of this compound and enhancing its in vitro toxicity. Apart from this metabolic interaction, no mixtures showed any interaction, and all binary mixtures were successfully modeled by at least one model based on exposure to the individual compounds. - Highlights: • We could predict cell response over repeated exposure to mixtures of cosmetics. • Compounds acted independently on the cells. • Metabolic interactions impacted exposure concentrations to the compounds.

  4. Differential T cell response against BK virus regulatory and structural antigens: A viral dynamics modelling approach.

    Science.gov (United States)

    Blazquez-Navarro, Arturo; Schachtner, Thomas; Stervbo, Ulrik; Sefrin, Anett; Stein, Maik; Westhoff, Timm H; Reinke, Petra; Klipp, Edda; Babel, Nina; Neumann, Avidan U; Or-Guil, Michal

    2018-05-01

    BK virus (BKV) associated nephropathy affects 1-10% of kidney transplant recipients, leading to graft failure in about 50% of cases. Immune responses against different BKV antigens have been shown to have a prognostic value for disease development. Data currently suggest that the structural antigens and regulatory antigens of BKV might each trigger a different mode of action of the immune response. To study the influence of different modes of action of the cellular immune response on BKV clearance dynamics, we have analysed the kinetics of BKV plasma load and anti-BKV T cell response (Elispot) in six patients with BKV associated nephropathy using ODE modelling. The results show that only a small number of hypotheses on the mode of action are compatible with the empirical data. The hypothesis with the highest empirical support is that structural antigens trigger blocking of virus production from infected cells, whereas regulatory antigens trigger an acceleration of death of infected cells. These differential modes of action could be important for our understanding of BKV resolution, as according to the hypothesis, only regulatory antigens would trigger a fast and continuous clearance of the viral load. Other hypotheses showed a lower degree of empirical support, but could potentially explain the clearing mechanisms of individual patients. Our results highlight the heterogeneity of the dynamics, including the delay between immune response against structural versus regulatory antigens, and its relevance for BKV clearance. Our modelling approach is the first that studies the process of BKV clearance by bringing together viral and immune kinetics and can provide a framework for personalised hypotheses generation on the interrelations between cellular immunity and viral dynamics.

  5. Analysis of real-time mixture cytotoxicity data following repeated exposure using BK/TD models

    Energy Technology Data Exchange (ETDEWEB)

    Teng, S.; Tebby, C. [Models for Toxicology and Ecotoxicology Unit, INERIS, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte (France); Barcellini-Couget, S. [ODESIA Neosciences, Sophia Antipolis, 400 route des chappes, 06903 Sophia Antipolis (France); De Sousa, G. [INRA, ToxAlim, 400 route des Chappes, BP, 167 06903 Sophia Antipolis, Cedex (France); Brochot, C. [Models for Toxicology and Ecotoxicology Unit, INERIS, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte (France); Rahmani, R. [INRA, ToxAlim, 400 route des Chappes, BP, 167 06903 Sophia Antipolis, Cedex (France); Pery, A.R.R., E-mail: alexandre.pery@agroparistech.fr [AgroParisTech, UMR 1402 INRA-AgroParisTech Ecosys, 78850 Thiverval Grignon (France); INRA, UMR 1402 INRA-AgroParisTech Ecosys, 78850 Thiverval Grignon (France)

    2016-08-15

    Cosmetic products generally consist of multiple ingredients. Thus, cosmetic risk assessment has to deal with mixture toxicity on a long-term scale which means it has to be assessed in the context of repeated exposure. Given that animal testing has been banned for cosmetics risk assessment, in vitro assays allowing long-term repeated exposure and adapted for in vitro – in vivo extrapolation need to be developed. However, most in vitro tests only assess short-term effects and consider static endpoints which hinder extrapolation to realistic human exposure scenarios where concentration in target organs is varies over time. Thanks to impedance metrics, real-time cell viability monitoring for repeated exposure has become possible. We recently constructed biokinetic/toxicodynamic models (BK/TD) to analyze such data (Teng et al., 2015) for three hepatotoxic cosmetic ingredients: coumarin, isoeugenol and benzophenone-2. In the present study, we aim to apply these models to analyze the dynamics of mixture impedance data using the concepts of concentration addition and independent action. Metabolic interactions between the mixture components were investigated, characterized and implemented in the models, as they impacted the actual cellular exposure. Indeed, cellular metabolism following mixture exposure induced a quick disappearance of the compounds from the exposure system. We showed that isoeugenol substantially decreased the metabolism of benzophenone-2, reducing the disappearance of this compound and enhancing its in vitro toxicity. Apart from this metabolic interaction, no mixtures showed any interaction, and all binary mixtures were successfully modeled by at least one model based on exposure to the individual compounds. - Highlights: • We could predict cell response over repeated exposure to mixtures of cosmetics. • Compounds acted independently on the cells. • Metabolic interactions impacted exposure concentrations to the compounds.

  6. Transplante cardíaco infantil: perspectivas e sentimentos maternos Trasplante cardiaco infantil: perspectivas y sentimientos maternos Heart transplant in children: maternal perspectives and feelings

    Directory of Open Access Journals (Sweden)

    Kiarelle Lourenço Penaforte

    2009-12-01

    Full Text Available Objetiva-se descrever as perspectivas maternas e sentimentos despertados pelo transplante cardíaco infantil. Estudo descritivo com abordagem qualitativa, desenvolvido no Hospital do Coração, de Messejana, em Fortaleza-CE, no período de agosto a novembro de 2007. Os sujeitos foram sete mães de crianças transplantadas. Utilizaram-se como técnicas a entrevista semiestruturada e a análise dos prontuários. Os dados foram submetidos à análise de conteúdo, em três etapas, segundo Bardin, organizados, analisados e interpretados à luz da literatura pertinente, vivência das pesquisadoras e experiência das entrevistadas. Da análise das falas, emergiram as unidades temáticas: necessidade do transplante; fonte de apoio; sentimentos externados pelas mães; a doação e perspectivas. Conclui-se que a doação é um momento de felicidade vivenciada por todas as mães, tratando-se da solução para vida de seus filhos, e a presença autêntica do enfermeiro nesta ocasião torna-se ainda mais essencial, pois humanizar faz parte do cuidar da enfermagem.El objetivo fue describir las perspectivas maternas y los sentimientos provocados por el trasplante cardiaco infantil. Estudio descriptivo con abordaje cualitativo, desarrollado en el Hospital del Corazón, de Messejana, en Fortaleza-CE, en el período de agosto a noviembre del 2007. Los sujetos del estudio fueron siete madres de niños con transplante . Se utilizó como técnica la entrevista semiestructurada y el análisis de los registros médicos. Los datos fueron sometidos al análisis del contenido, en tres etapas, según Bardin, siendo organizados, analizados e interpretados a la luz de la bibliografía pertinente, de la vivencia de las investigadoras y de la experiencia de las entrevistadas. Del análisis del discurso , surgieron las unidades temáticas la necesidad del trasplante; las fuente de ayuda; los sentimientos expresados por las madres; la donación y sus perspectivas. Se concluy

  7. Costo-utilidad del trasplante renal frente a la hemodiálisis en el tratamiento de la insuficiencia renal crónica terminal en un hospital peruano Cost utility of renal transplant vs. hemodialysis in the treatment of end stage chronic kidney failure in a peruvian hospital

    Directory of Open Access Journals (Sweden)

    Ricardo E. Loza-Concha

    2011-09-01

    Full Text Available Objetivos. Determinar y comparar las razones de costo-utilidad de los trasplantes renales con donante cadavérico (TRDC practicados en el Hospital Nacional Guillermo Almenara Irigoyen los años 2000-2001, frente a hemodiálisis (HD, cinco años después del inicio del tratamiento. Material y métodos. Se realizó un estudio de costo utilidad, estudiando a todos los pacientes continuadores cinco años post TRDC, a quienes se asignó como controles, dos pacientes con cinco años de HD pareados por edad, sexo y tiempo de enfermedad. Se evaluaron los costos de cada procedimiento, los años de vida ajustados a la calidad (AVAC utilizando el cuestionario SF-36v2TM y se calcularon las razones de costo utilidad (CU y costo utilidad incremental (CUI. Resultados. Se realizaron 58 TRDC los años 2000-2001. Cinco años después, 17 (29 % pacientes fallecieron y 27 (47 % continuaron con su tratamiento post-TRDC. Treinta y uno (53 % TRDC fracasaron; 26 % por rechazo al trasplante, 55 % por complicación y 19 % por tratamiento irregular. Los puntajes SF-36v2TM promedio obtenidos por los TRDC y HD fueron 95±13 y 87±18 puntos respectivamente. En ambos años, los AVAC obtenidos por los TRDC y HD fueron de 251 y 229 puntos respectivamente; las razones de CU para los TRDC y los HD fueron de USD 11 984 y USD 9243, y la razón de CUI fue de USD 40 669. Conclusiones. Los TRDC realizados los años 2000-2001, cinco años después resultaron menos costo útiles que las HD; y los TRDC realizados el 2000 tuvieron una menor razón de costo utilidad incremental que los efectuados el 2001, probablemente por su mayor razón de tratamientos irregulares.Objectives. To assess and compare the cost utility of the cadaveric donor renal transplant (CDRT at the Hospital Nacional Guillermo Almenara Irigoyen between 2000 to 2001, against haemodialysis (HD, 5 years after treatment initiation. Materials and Methods. A cost utility study design was used, which evaluated every patient

  8. Trasplante de médula ósea: abordaje de la Terapia Ocupacional = Transplant of bony marrow : boarding th Occupational Theapy

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    Alahmar Bianchin, M

    2007-09-01

    Full Text Available ResumenEl trasplante de médula ósea (TMO es una modalidad terapéutica que se utiliza en una variedad de enfermedades oncológicas y hematológicas, con el objetivo de prolongar la remisión completa o la cura de la enfermedad. La unidad de TMO del Hospital de Base de San José Del Río Preto - San Pablo, Brasil, cuenta con un equipo formado por doctores, enfermeros, terapeuta ocupacional, psicólogo, nutricionista y asistente social. El estudio presente tiene por objetivo describir la importancia de la intervención de la Terapia Ocupacional a través del uso de actividades en la fase de aislamiento que conlleva el TMO, proporcionando así, una mejor aceptación y adaptación en relación al tratamiento SummaryBone Marrow Transplant is a therapeutical procedure that has been very used in a variety of oncological illnesses, also in hereditary and acquired hematologica illnesses, whith the objective to draw out illness complete remission or the cure. During the isolation of bone marrow transplant unit, many are the psycopathological reartions that patients can present in treatment adaptation period. With the intention of assisting the customer during this phase, the occupational therapy aims at this context, to supply a quality treatment, through activities with objective to raise they self- esteern, to diminish the ideness, to reduce stress it among othres generated for the illness. Therefore when carrying though much activities the costumer will be able to live since a simple satisfaction moment lentil one better acceptance and treatment adaptation.

  9. Trasplante de células progenitoras hematopoyéticas en enfermedades autoinmunes Hematopoietic stem cell transplantation in autoimmune diseases

    Directory of Open Access Journals (Sweden)

    Flavio Albarracín

    2008-04-01

    Full Text Available El trasplante de células progenitoras hematopoyéticas, células con capacidad de autorrenovación y reconstitución de todos los tipos de células sanguíneas, se utiliza en el tratamiento de numerosas enfermedades potencialmente letales incluyendo leucemias y linfomas. Hoy en día es posible además aplicarlo en el tratamiento de enfermedades autoinmunes graves, como esclerosis múltiple, lupus eritematoso sistémico o esclerosis sistémica, resistentes a la terapia convencional. Estudios en modelos animales nos demuestran que la transferencia de células progenitoras hematopoyéticas podría revertir el proceso de autoinmunidad, un fenómeno que puede explicarse mediante diferentes mecanismos. El resultado de los estudios clínicos que se están llevando a cabo, así como también estudios en pacientes y modelos animales, ayudarán a determinar el rol que el transplante de células progenitoras hematopoyéticas puede jugar en el tratamiento de enfermedades autoinmunes.Transplantation of hematopoietic stem cells, which are capable of self renewal and reconstitution of all types of blood cells, can be a treatment for numerous potential lethal diseases, including leukemias and lymphomas. It may now be applicable for the treatment of severe autoimmune diseases, such as therapy-resistant multiple sclerosis, lupus and systemic sclerosis. Studies in animal models show that the transfer of hematopoietic stem cells can reverse autoimmunity. The outcome of ongoing clinical trials, as well as of studies in patients and animal models, will help to determine the role that stem-cell transplantation can play in the treatment of autoimmune diseases.

  10. Cholesterol influences voltage-gated calcium channels and BK-type potassium channels in auditory hair cells.

    Directory of Open Access Journals (Sweden)

    Erin K Purcell

    Full Text Available The influence of membrane cholesterol content on a variety of ion channel conductances in numerous cell models has been shown, but studies exploring its role in auditory hair cell physiology are scarce. Recent evidence shows that cholesterol depletion affects outer hair cell electromotility and the voltage-gated potassium currents underlying tall hair cell development, but the effects of cholesterol on the major ionic currents governing auditory hair cell excitability are unknown. We investigated the effects of a cholesterol-depleting agent (methyl beta cyclodextrin, MβCD on ion channels necessary for the early stages of sound processing. Large-conductance BK-type potassium channels underlie temporal processing and open in a voltage- and calcium-dependent manner. Voltage-gated calcium channels (VGCCs are responsible for calcium-dependent exocytosis and synaptic transmission to the auditory nerve. Our results demonstrate that cholesterol depletion reduced peak steady-state calcium-sensitive (BK-type potassium current by 50% in chick cochlear hair cells. In contrast, MβCD treatment increased peak inward calcium current (~30%, ruling out loss of calcium channel expression or function as a cause of reduced calcium-sensitive outward current. Changes in maximal conductance indicated a direct impact of cholesterol on channel number or unitary conductance. Immunoblotting following sucrose-gradient ultracentrifugation revealed BK expression in cholesterol-enriched microdomains. Both direct impacts of cholesterol on channel biophysics, as well as channel localization in the membrane, may contribute to the influence of cholesterol on hair cell physiology. Our results reveal a new role for cholesterol in the regulation of auditory calcium and calcium-activated potassium channels and add to the growing evidence that cholesterol is a key determinant in auditory physiology.

  11. Experimental Study of Tool Wear and Grinding Forces During BK-7 Glass Micro-grinding with Modified PCD Tool

    Science.gov (United States)

    Pratap, A.; Sahoo, P.; Patra, K.; Dyakonov, A. A.

    2017-09-01

    This study focuses on the improvement in grinding performance of BK-7 glass using polycrystalline diamond micro-tool. Micro-tools are modified using wire EDM and performance of modified tools is compared with that of as received tool. Tool wear of different types of tools are observed. To quantify the tool wear, a method based on weight loss of tool is introduced in this study. Modified tools significantly reduce tool wear in comparison to the normal tool. Grinding forces increase with machining time due to tool wear. However, modified tools produce lesser forces thus can improve life of the PCD micro-grinding tool.

  12. 哈维氏弧菌黑鲷分离株BK-1培养条件优化研究%Studies on the Optimal Culture Conditions of Vibrio harveyi BK-1 from Sparus macrocephalus

    Institute of Scientific and Technical Information of China (English)

    潘晓艺; 沈锦玉; 尹文林; 曹铮; 马海其; 常抗美

    2005-01-01

    从患病黑鲷Sparus macrocephalus 的肾脏分离到一致病菌株BK-1,经鉴定为哈维氏弧菌.对哈维氏弧菌BK-1株的最佳生长条件及培养基优化进行了测定.结果表明:不同的培养条件和培养基成份均会影响其产量.哈维氏弧菌最适宜生长条件为:盐度为2%、pH8、温度为30℃;最佳培养基成份为:蛋白胨0.5%,牛肉膏0.75%,甘油0.05%,CuSO40.15 mg/L,CaCl2 0.01 g/L.

  13. Unraveling hominin behavior at another anthropogenic site from Olduvai Gorge (Tanzania): new archaeological and taphonomic research at BK, Upper Bed II.

    Science.gov (United States)

    Domínguez-Rodrigo, M; Mabulla, A; Bunn, H T; Barba, R; Diez-Martín, F; Egeland, C P; Espílez, E; Egeland, A; Yravedra, J; Sánchez, P

    2009-09-01

    New archaeological excavations and research at BK, Upper Bed II (Olduvai Gorge, Tanzania) have yielded a rich and unbiased collection of fossil bones. These new excavations show that BK is a stratified deposit formed in a riverine setting close to an alluvial plain. The present taphonomic study reveals the second-largest collection of hominin-modified bones from Olduvai, with abundant cut marks found on most of the anatomical areas preserved. Meat and marrow exploitation is reconstructed using the taphonomic signatures left on the bones by hominins. Highly cut-marked long limb shafts, especially those of upper limb bones, suggest that hominins at BK were actively engaged in acquiring small and middle-sized animals using strategies other than passive scavenging. The exploitation of large-sized game (Pelorovis) by Lower Pleistocene hominins, as suggested by previous researchers, is supported by the present study.

  14. Impact of low-level BK polyomavirus viremia on intermediate-term renal allograft function.

    Science.gov (United States)

    Korth, Johannes; Widera, Marek; Dolff, Sebastian; Guberina, Hana; Bienholz, Anja; Brinkhoff, Alexandra; Anastasiou, Olympia Evdoxia; Kribben, Andreas; Dittmer, Ulf; Verheyen, Jens; Wilde, Benjamin; Witzke, Oliver

    2018-02-01

    BK polyomavirus (BKPyV)-associated nephropathy (PyVAN) is a significant cause of premature renal transplant failure. High-level BKPyV viremia is predictive for PyVAN; however, low-level BKPyV viremia does not necessarily exclude the presence of PyVAN. As data are limited regarding whether or not low-level BKPyV viremia has an effect on intermediate-term graft outcome, this study analyzes the impact of low-level BKPyV viremia on intermediate-term graft function and outcome compared with high-level viremia and non-viremic patients. All renal transplant patients received follow-up examinations at the Department of Nephrology, University Hospital Essen. Patients were screened for BKPyV viremia and stratified into three groups according to their maximum BKPyV load in serum (low-level viremia, high-level viremia, and no viremia). In 142 of 213 (67%) patients, BKPyV was never detected in serum; 42 of 213 (20%) patients were found positive for low-level viremia (≤10 4 copies/mL); and 29 of 213 (13%) patients showed high-level viremia (>10 4 copies/mL). No significant differences regarding transplant function and graft failure were observed between patients without BKPyV viremia (delta estimated glomerular filtration rate [eGFR] +0.1 mL/min [month 1 vs last visit at month 44]) and patients with low-level BKPyV viremia (delta eGFR -1.7 mL/min). In patients with high-level viremia, transplant function was significantly restricted (delta eGFR -6.5 mL/min) compared with low-level viremia until the last visit at 44 ± 9.7 months after transplantation. Although the graft function and graft loss were worse in the high-level viremia group compared with no viremia (eGFR 37 vs 45 mL/min), the difference was not significant. High-level viremia was associated with impaired graft function. In contrast, low-level BKPyV viremia had no significant impact on intermediate-term graft function. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  15. Perilaku Bullying dan Peranan Guru BK/Konselor dalam Pengentasannya (Studi Deskriptif terhadap Siswa SMP Negeri 3 Lubuk Basung

    Directory of Open Access Journals (Sweden)

    Ilfajri Yenes

    2016-06-01

    Full Text Available Bullying merupakan suatu situasi di mana terjadinya penyalahgunaan kekuatan/kekuasaan yang dilakukan oleh seseorang/kelompok. Perilaku bullying merupakan  salah satu bentuk kekerasan dan agresif siswa di sekolah. Penelitian ini adalah penelitian deskriptif yang bertujuan untuk mendeskripsikan perilaku bullying yang ditampilkan siswa SMPN 3 Lubuk Basung dan peranan guru BK/konselor dalam pengentasannya. Populasi penelitian adalah siswa di SMP Negeri 3 Lubuk Basung berjumlah  564 orang dengan sampel 138 orang diambil dengan menggunakan teknik propotonal stratified random. Teknik pengumpulan data dengan menggunakan angket. Temuan penelitian menunjukkan bahwa jenis perilaku bullying yang dominan terjadi yaitu menyakiti secara verbal dilanjutkan dengan menyakiti secara fisik kemudian menyakiti secara mental dengan faktor keluarga. Faktor teman sebaya merupakan faktor yang lebih dominan sebagai penyebab perilaku bullying siswa. Secara umum guru BK/konselor cukup berperan mengatasi perilaku bullyingdengan memberikan  layanan informasi sebagai layanan yang lebih dominan diberikan dilanjutkan dengan layanan bimbingan kelompok dan konseling kelompok serta pemberian layanan konseling individual.

  16. Polyomavirus BK replication in renal transplant recipients: combined monitoring of viremia and VP1 mRNA in urine

    Directory of Open Access Journals (Sweden)

    Sara Astegiano

    2010-06-01

    Full Text Available Introduction. Human polyomavirus BK (BKV is worldwide distributed, with a seroprevalence rate of 70–90% in the adults. Following primary infection, BK remains latent in the renourinary tract as the epidemiologically most relevant latency site, and in B cell, brain, spleen and probably other tissues. Reactivation may occur in both immunocompetent subjects and immunocompromised patients. In renal transplantation, in the context of intense immunosuppression, viral replication may determine BKV-associated nephropathy (BKVAN with interstitial nephritis and/or ureteral stenosis in 1–10% of the patients and leading to graft failure and return to haemodialysis in 30 to 80% of the cases (5. Screening of BKV replication represents the basic strategy to predict early the onset of BKVAN and may allow for earlier intervention with reduced allograft loss (3, 4. Nowadays, replication of BKV is monitored by quantification of BKV-DNA in serum and urine (2. The aim of this study was to evaluated the role of BKV VP1 mRNA in urine as a marker of viral replication in renal transplant recipients.

  17. Experimental Investigation on Cutting Characteristics in Nanometric Plunge-Cutting of BK7 and Fused Silica Glasses.

    Science.gov (United States)

    An, Qinglong; Ming, Weiwei; Chen, Ming

    2015-03-27

    Ductile cutting are most widely used in fabricating high-quality optical glass components to achieve crack-free surfaces. For ultra-precision machining of brittle glass materials, critical undeformed chip thickness (CUCT) commonly plays a pivotal role in determining the transition point from ductile cutting to brittle cutting. In this research, cutting characteristics in nanometric cutting of BK7 and fused silica glasses, including machined surface morphology, surface roughness, cutting force and specific cutting energy, were investigated with nanometric plunge-cutting experiments. The same cutting speed of 300 mm/min was used in the experiments with single-crystal diamond tool. CUCT was determined according to the mentioned cutting characteristics. The results revealed that 320 nm was found as the CUCT in BK7 cutting and 50 nm was determined as the size effect of undeformed chip thickness. A high-quality machined surface could be obtained with the undeformed chip thickness between 50 and 320 nm at ductile cutting stage. Moreover, no CUCT was identified in fused silica cutting with the current cutting conditions, and brittle-fracture mechanism was confirmed as the predominant chip-separation mode throughout the nanometric cutting operation.

  18. Efecto del déficit de agua en el trasplante de plántulas de tomate (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    Balaguera Helber Enrique

    2008-08-01

    Full Text Available

    La inducción de la mayor cantidad de raíces en plántulas de tomate es fundamental en la etapa postrasplante, ya que de éstas depende la adecuada absorción de agua y nutrientes para su posterior desarrollo. El estrés hídrico puede generar un crecimiento radicular, ya que la raíz debe buscar agua. Con el fin de lograr este crecimiento, se sometieron plántulas de tomate a un déficit hídrico. El diseño fue completamente al azar con 10 tratamientos y 5 repeticiones; los tratamientos consistieron en la aplicación de 10 láminas de riego (0,2; 0,4; 0,6; 0,8; 1,0; 1,2; 1,4; 1,6; 1,8 y 2,0 de la evaporación medida en un tanque evaporímetro instalado dentro del invernadero durante los 5 días previos al trasplante. Se trasplantaron 40 d después de la siembra y se evaluaron un mes después. No se presentaron diferencias significativas en el peso seco de hojas, tallos y raíces. Las diferentes láminas de agua no afectaron el peso fresco de hojas y tallos, el área foliar, la distribución de fitomasa seca, la relación raíz/parte aérea ni la altura final de las plantas. Se obtuvieron diferencias significativas en la longitud y en el peso fresco de la raíz; en ambos casos el mejor tratamiento fue el coeficiente 1,6 de la evaporación. La lámina con menor respuesta fue la de 0,2. Una lámina de riego de 1,6 de la evaporación genera plántulas con sistemas radiculares adecuados.

  19. Astaxanthin and Docosahexaenoic Acid Reverse the Toxicity of the Maxi-K (BK Channel Antagonist Mycotoxin Penitrem A

    Directory of Open Access Journals (Sweden)

    Amira A. Goda

    2016-11-01

    Full Text Available Penitrem A (PA is a food mycotoxin produced by several terrestrial and few marine Penicillium species. PA is a potent tremorgen through selective antagonism of the calcium-dependent potassium BK (Maxi-K channels. Discovery of natural products that can prevent the toxic effects of PA is important for food safety. Astaxanthin (AST is a marine natural xanthophyll carotenoid with documented antioxidant activity. Unlike other common antioxidants, AST can cross blood brain barriers (BBBs, inducing neuroprotective effects. Docosahexaenoic acid (DHA is polyunsaturated ω-3 fatty acid naturally occurring in fish and algae. DHA is essential for normal neurological and cellular development. This study evaluated the protective activity of AST and DHA against PA-induced toxicity, in vitro on Schwann cells CRL-2765 and in vivo in the worm Caenorhbitidis elegans and Sprague Dawley rat models. PA inhibited the viability of Schwann cells, with an IC50 of 22.6 μM. Dose-dependent treatments with 10–100 μM DHA significantly reversed the PA toxicity at its IC50 dose, and improved the survival of Schwann cells to 70.5%–98.8%. Similarly, dose-dependent treatments with 10–20 μM AST reversed the PA toxicity at its IC50 dose and raised these cells’ survival to 61.7%–70.5%. BK channel inhibition in the nematode C. elegans is associated with abnormal reversal locomotion. DHA and AST counteracted the in vivo PA BK channel antagonistic activity in the C. elegans model. Rats fed a PA-contaminated diet showed high levels of glutamate (GLU, aspartate (ASP, and gamma amino butyric acid (GABA, with observed necrosis or absence of Purkinjie neurons, typical of PA-induced neurotoxicity. Dopamine (DA, serotonin (5-HT, and norepinephrine (NE levels were abnormal, Nitric Oxide (NO and Malondialdehyde (MDA levels were significantly increased, and total antioxidant capacity (TAC level in serum and brain homogenates was significantly decreased in PA-treated rats. DHA and AST

  20. Expression of BKCa channels and the modulatory ß-subunits in the rat and porcine trigeminal ganglion

    DEFF Research Database (Denmark)

    Wulf-Johansson, Helle; Hay-Schmidt, Anders; Poulsen, Asser Nyander

    2009-01-01

    Large conductance calcium-activated potassium (BK(Ca)) channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. Migraine has been proposed to be a trigeminovascular disease involving the sensory trigeminal pathways and the c...

  1. Recurrence of hepatocellular carcinoma after liver transplantation presenting as anastomotic biliary stricture Presentación del carcinoma hepatocelular recurrente tras el trasplante de hígado en forma de estenosis biliar anastomótica

    Directory of Open Access Journals (Sweden)

    S. Y. Chen

    2008-07-01

    Full Text Available A 52-year-old man visited our hospital complaining of anorexia and fatigue two months after receiving orthotopic liver transplantation for hepatocellular carcinoma. A laboratory investigation demonstrated a clinical picture of obstructive jaundice. T-tube cholangiography showed biliary stricture over the anastomotic site. Percutaneous transluminal balloon dilatation and stenting was attempted but failed. Magnetic resonance cholangiography showed possible tumor recurrence over the site of the anastomotic biliary stricture. A biopsy sample was obtained via ultrasound-guided aspiration and histopathological study revealed inflammatory and fibrotic changes. With high suspicion of recurrence of the hepatocellular carcinoma, surgical exploration was performed and an intraoperative frozen section proved the recurrence. We thus diagnosed this case as a recurrence of hepatocellular carcinoma after liver transplantation. To our knowledge, there have been no previous reports of early tumor recurrence after liver transplantation being the cause of an anastomotic biliary stricture.Un varón de 52 años visitó nuestro hospital quejándose de anorexia y fatiga a los dos meses de haber recibido un trasplante hepático ortotópico a causa de un carcinoma hepatocelular. La analítica mostró un cuadro clínico de ictericia obstructiva. La colangiografía con tubo en T mostró una estenosis biliar sobre la anastomosis. Se intentó una dilatación transluminal percutánea con globo y colocación de endoprótesis, que fracasó. La colangiografía por resonancia magnética mostró una posible recurrencia tumoral sobre el lugar de la estenosis biliar anastomótica. Se extrajo una muestra de biopsia mediante aspiración bajo guía ecográfica y el estudio histopatológico mostró alteraciones inflamatorias y fibróticas. Al sospecharse la recurrencia del carcinoma hepatocelular, se realizó una exploración quirúrgica; un corte intraoperatorio congelado demostr

  2. The Role of Ca2+ and BK Channels of Locus Coeruleus (LC) Neurons as a Brake to the CO2 Chemosensitivity Response of Rats.

    Science.gov (United States)

    Imber, Ann N; Patrone, Luis G A; Li, Ke-Yong; Gargaglioni, Luciane H; Putnam, Robert W

    2018-06-15

    The cellular mechanisms by which LC neurons respond to hypercapnia are usually attributed to an "accelerator" whereby hypercapnic acidosis causes an inhibition of K + channels or activation of Na + and Ca +2 channels to depolarize CO 2 -sensitive neurons. Nevertheless, it is still unknown if this "accelerator" mechanism could be controlled by a brake phenomenon. Whole-cell patch clamping, fluorescence imaging microscopy and plethysmography were used to study the chemosensitive response of the LC neurons. Hypercapnic acidosis activates L-type Ca 2+ channels and large conductance Ca-activated K + (BK) channels, which function as a "brake" on the chemosensitive response of LC neurons. Our findings indicate that both Ca 2+ and BK currents develop over the first 2 weeks of postnatal life in rat LC slices and that this brake pathway may cause the developmental decrease in the chemosensitive firing rate response of LC neurons to hypercapnic acidosis. Inhibition of this brake by paxilline (BK channel inhibitor) returns the magnitude of the chemosensitive firing rate response from LC neurons in rats older than P10 to high values similar to those in LC neurons from younger rats. Inhibition of BK channels in LC neurons by bilateral injections of paxilline into the LC results in a significant increase in the hypercapnic ventilatory response of adult rats. Our findings indicate that a BK channel-based braking system helps to determine the chemosensitive respiratory drive of LC neurons and contributes to the hypercapnic ventilatory response. Perhaps, abnormalities of this braking system could result in hypercapnia-induced respiratory disorders and panic responses. Copyright © 2018 IBRO. Published by Elsevier Ltd. All rights reserved.

  3. Statin therapy exacerbates alcohol-induced constriction of cerebral arteries via modulation of ethanol-induced BK channel inhibition in vascular smooth muscle.

    Science.gov (United States)

    Simakova, Maria N; Bisen, Shivantika; Dopico, Alex M; Bukiya, Anna N

    2017-12-01

    Statins constitute the most commonly prescribed drugs to decrease cholesterol (CLR). CLR is an important modulator of alcohol-induced cerebral artery constriction (AICAC). Using rats on a high CLR diet (2% CLR) we set to determine whether atorvastatin administration (10mg/kg daily for 18-23weeks) modified AICAC. Middle cerebral arteries were pressurized in vitro at 60mmHg and AICAC was evoked by 50mM ethanol, that is within the range of blood alcohol detected in humans following moderate-to-heavy drinking. AICAC was evident in high CLR+atorvastatin group but not in high CLR diet+placebo. Statin exacerbation of AICAC persisted in de-endothelialized arteries, and was blunted by CLR enrichment in vitro. Fluorescence imaging of filipin-stained arteries showed that atorvastatin decreased vascular smooth muscle (VSM) CLR when compared to placebo, this difference being reduced by CLR enrichment in vitro. Voltage- and calcium-gated potassium channels of large conductance (BK) are known VSM targets of ethanol, with their beta1 subunit being necessary for ethanol-induced channel inhibition and resulting AICAC. Ethanol-induced BK inhibition in excised membrane patches from freshly isolated myocytes was exacerbated in the high CLR diet+atorvastatin group when compared to high CLR diet+placebo. Unexpectedly, atorvastatin decreased the amount and function of BK beta1 subunit as documented by immunofluorescence imaging and functional patch-clamp studies. Atorvastatin exacerbation of ethanol-induced BK inhibition disappeared upon artery CLR enrichment in vitro. Our study demonstrates for the first time statin's ability to exacerbate the vascular effect of a widely consumed drug of abuse, this exacerbation being driven by statin modulation of ethanol-induced BK channel inhibition in the VSM via CLR-mediated mechanism. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. A comparison of HK-CONWIP and BK-CONWIP control strategies in a multi-product manufacturing system

    Directory of Open Access Journals (Sweden)

    Chukwunonyelum Emmanuel Onyeocha

    2015-12-01

    Full Text Available This paper evaluates the performance of the Hybrid Kanban Constant Work-In-Process control strategy and Basestock Kanban Constant Work-In-Process control strategy operating Shared Kanban Allocation Policy (S-KAP and Dedicated Kanban Allocation Policy (D-KAP in a multi-product serial flow line. We explored the effect of an increase of product types on the WIP inventory in the system. A simulation-based optimisation technique was used in determining the optimal settings for the strategies. The strategies were compared via pairwise comparison technique and Nelson’s ranking and selection procedure. S-KAP responds quicker to demand than D-KAP. BK-CONWIP outperforms HK-CONWIP in a serial manufacturing system. It was shown that an increase in the number of product-type increases the number of PAC and WIP inventory.

  5. Broadening of white-light continuum by filamentation in BK7 glass at its zero-dispersion point

    International Nuclear Information System (INIS)

    Jiang, Jiaming; Zhong, Yue; Zheng, Yinghui; Zeng, Zhinan; Ge, Xiaochun; Li, Ruxin

    2015-01-01

    Broadening of white-light continuum is observed by filamentation of near-infrared femtosecond laser pulses with peak power exceeding the megawatt level in BK7 glass with the presence of the zero-dispersion point. The simulated results show that, due to the low dispersion at the zero-dispersion point, the broadening of white-light continuum can be wider and the filament can persist in propagating stably longer distance. - Highlights: • We observed the white-light continuum by filamentation at the zero-dispersion point. • Peak power significantly exceeding the critical value of self-focusing was used. • Wider spectral broadening was obtained around the zero-dispersion point

  6. No evidence for infection of UK prostate cancer patients with XMRV, BK virus, Trichomonas vaginalis or human papilloma viruses.

    Science.gov (United States)

    Groom, Harriet C T; Warren, Anne Y; Neal, David E; Bishop, Kate N

    2012-01-01

    The prevalence of specific infections in UK prostate cancer patients was investigated. Serum from 84 patients and 62 controls was tested for neutralisation of xenotropic murine leukaemia virus-related virus (XMRV) Envelope. No reactivity was found in the patient samples. In addition, a further 100 prostate DNA samples were tested for XMRV, BK virus, Trichomonas vaginalis and human papilloma viruses by nucleic acid detection techniques. Despite demonstrating DNA integrity and assay sensitivity, we failed to detect the presence of any of these agents in DNA samples, bar one sample that was weakly positive for HPV16. Therefore we conclude that these infections are absent in this typical cohort of men with prostate cancer.

  7. No evidence for infection of UK prostate cancer patients with XMRV, BK virus, Trichomonas vaginalis or human papilloma viruses.

    Directory of Open Access Journals (Sweden)

    Harriet C T Groom

    Full Text Available The prevalence of specific infections in UK prostate cancer patients was investigated. Serum from 84 patients and 62 controls was tested for neutralisation of xenotropic murine leukaemia virus-related virus (XMRV Envelope. No reactivity was found in the patient samples. In addition, a further 100 prostate DNA samples were tested for XMRV, BK virus, Trichomonas vaginalis and human papilloma viruses by nucleic acid detection techniques. Despite demonstrating DNA integrity and assay sensitivity, we failed to detect the presence of any of these agents in DNA samples, bar one sample that was weakly positive for HPV16. Therefore we conclude that these infections are absent in this typical cohort of men with prostate cancer.

  8. Production and decay of the heaviest odd-Z nuclei in the 249Bk + 48Ca reaction

    International Nuclear Information System (INIS)

    Oganessian, Yu Ts; Abdullin, F Sh; Dmitriev, S N; Itkis, M G; Polyakov, A N; Alexander, C; Binder, J; Boll, R A; Ezold, J; Felker, K; Grzywacz, R K; Miernik, K; Roberto, J B; Gostic, J M; Henderson, R A; Moody, K J; Hamilton, J H; Ramayya, A V; Miller, D; Ryabinin, M A

    2015-01-01

    The reaction of 249 Bk with 48 Ca has been investigated with an aim of synthesizing and studying the decay properties of isotopes of the new element 117. The experiments were performed at five projectile energies (in two runs, in 2009-2010 and 2012) and with a total beam dose of 48 Ca ions of about 9x10 19 The experiments yielded data on a-decay characteristics and excitation functions of the produced nuclei that establish these to be 293 117 and 294 117 – the products of the 4n- and 3n-evaporation channels, respectively. In total, we have observed 20 decay chains of Z=117 nuclides. The cross sections were measured to be 1.1 pb for the 3n and 2.4 pb for the 4n-reaction channel. The new 289 115 events, populated by α decay of 117, demonstrate the same decay properties as those observed for 115 produced in the 243 Am( 48 Ca,2n) reaction thus providing cross-bombardment evidence. In addition, a single decay of 294 118 was observed from the reaction with 249 Cf – a result of the in-growth of 249 Cf in the 249 Bk target. The observed decay chain of 294 118 is in good agreement with decay properties obtained in 2002-2005 in the experiments with the reaction 249 Cf( 48 Ca,3n) 294 118. The energies and half-lives of the odd-Z isotopes observed in the 117 decay chains together with the results obtained for lower-Z superheavy nuclei demonstrate enhancement of nuclear stability with increasing neutron number towards the predicted new magic number N=184

  9. Aplicación de técnicas de simulación de bajo coste en un sistema de eLearning en la Organización Nacional de Trasplantes

    OpenAIRE

    Borro Escribano, Blanca

    2010-01-01

    La Organización Nacional de Trasplantes ha logrado desde su origen en el año 1989 que España pase a ser el país con mayor número de donaciones por millón de habitantes convirtiéndose en el centro de atención de todos aquellos países que tienen un sistema sanitario evolucionado. Estos países quieren aprender del ya conocido como “Modelo Español”, con el objetivo de disminuir las largas listas de espera de receptores. Este trabajo describe el diseño, planificación, sistematización y despl...

  10. Resultados de una encuesta sobre donación y trasplante de órganos en la zona metropolitana de Guadalajara, México

    Directory of Open Access Journals (Sweden)

    Zepeda-Romero Luz Consuelo

    2003-01-01

    Full Text Available OBJETIVO: Evaluar el conocimiento y la disposición de la donación de órganos y tejidos con fines de trasplante entre la población adulta del área metropolitana de Guadalajara, Jalisco, México. MATERIAL Y MÉTODOS: Se realizó una encuesta aplicando un cuestionario de 29 reactivos a personas mayores de 18 años de edad, mediante entrevista directa en la vía pública. La zona metropolitana se dividió en cuatro municipios, asignándose a cada uno de éstos el número de encuestas en proporción al número de habitantes, estrato socioeconómico, edad y género. Se calcularon frecuencias y ji2 para diferencias de proporciones. RESULTADOS: Se encuestaron 400 individuos. El 80.4% conocía acerca de la donación de órganos y 65% sabía que está permitido por la ley; sin embargo, solamente 24% había comentado el tema en familia. El 71% de los encuestados desconocía cómo donar sus órganos y sólo 25% sabía de la existencia de la tarjeta de donación. Por otra parte, 66% estarían dispuestos a donarlos, 16.5% no lo harían y 17% estaban indecisos. La mutilación corporal fue la razón principal (40% para no donar. El 89% estarían dispuestos a autorizar la extracción de órganos de un familiar, siempre que éste hubiera manifestado su deseo en vida; de lo contrario, sólo 29% procederían con la autorización. Finalmente, 65% consideró la licencia de conducir como un medio adecuado de expresar el deseo de donar y 50% considera insuficiente la información que sobre el tema se ha difundido por los medios de comunicación. CONCLUSIONES: La mayoría de la población de la zona metropolitana de la ciudad de Guadalajara tiene conocimiento sobre la donación de órganos y estaría dispuesta a donarlos. Sin embargo, un alto porcentaje desconoce el cómo hacerlo y considera insuficiente la información difundida sobre el tema. Una campaña permanente a través de los medios de comunicación masiva podría solucionar lo anterior e incrementaría la

  11. Factores pronósticos de complicaciones postoperatorias en el trasplante hepático Prognostic factors associated with postoperative complications in liver transplantation

    Directory of Open Access Journals (Sweden)

    A. Rodríguez-Ariza

    2008-03-01

    Full Text Available Objetivo: la evolución postoperatoria de los pacientes sometidos a trasplante hepático ortotópico (THO se encuentra frecuentemente asociada a la aparición de diversas complicaciones tales como disfunción renal, rechazo agudo, infecciones y complicaciones neurológicas. Estas complicaciones constituyen las causas más significativas de morbilidad y mortalidad tempranas en pacientes que reciben un THO. El propósito del presente estudio es la identificación de factores relacionados con las distintas complicaciones postoperatorias del THO. Diseño experimental: se llevó a cabo un estudio prospectivo. Pacientes: se analizaron 78 variables en 32 pacientes consecutivos sometidos a THO. Utilizando un análisis de regresión logística se identificaron aquellos factores asociados de forma independiente con la aparición de complicaciones postoperatorias. Resultados: el análisis multivariante demostró que los niveles pretrasplante en suero de malondialdehído y creatinina estaban asociados con el desarrollo de disfunción renal. Los niveles pretrasplante de hemoglobina y las unidades de plaquetas administradas durante la cirugía fueron factores pronósticos de infecciones. El rechazo agudo fue pronosticado por los niveles séricos de γ-glutamil transpeptidasa y de bilirrubina total. Los niveles pretrasplante de sodio y glutaredoxina en suero estuvieron asociados con complicaciones neurológicas. Conclusiones: proponemos estos marcadores para la identificación de pacientes de alto riesgo, permitiendo una vigilancia y/o tratamiento anticipados que mejorarán la morbilidad y la supervivencia en pacientes sometidos a THO.Objectives: the postoperative evolution of patients submitted to orthotopic liver transplant (OLT is frequently associated with the appearance of different types of complications such as renal failure, graft rejection, infections, and neurological disorders. These complications are the most significant causes of early morbidity

  12. The first transmembrane domain (TM1) of β2-subunit binds to the transmembrane domain S1 of α-subunit in BK potassium channels

    Science.gov (United States)

    Morera, Francisco J.; Alioua, Abderrahmane; Kundu, Pallob; Salazar, Marcelo; Gonzalez, Carlos; Martinez, Agustin D.; Stefani, Enrico; Toro, Ligia; Latorre, Ramon

    2012-01-01

    The BK channel is one of the most broadly expressed ion channels in mammals. In many tissues, the BK channel pore-forming α-subunit is associated to an auxiliary β-subunit that modulates the voltage- and Ca2+-dependent activation of the channel. Structural components present in β-subunits that are important for the physical association with the α-subunit are yet unknown. Here, we show through co-immunoprecipitation that the intracellular C-terminus, the second transmembrane domain (TM2) and the extracellular loop of the β2-subunit are dispensable for association with the α-subunit pointing transmembrane domain 1 (TM1) as responsible for the interaction. Indeed, the TOXCAT assay for transmembrane protein–protein interactions demonstrated for the first time that TM1 of the β2-subunit physically binds to the transmembrane S1 domain of the α-subunit. PMID:22710124

  13. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA

    DEFF Research Database (Denmark)

    Christensen, S.K.; Pedersen, K.; Hansen, Flemming G.

    2003-01-01

    Prokaryotic chromosomes encode toxin-antitoxin loci, often in multiple copies. In most cases, the function of these genes is not known. The chpA (mazEF) locus of Escherichia coli has been described as a cell killing module that induces bacterial apoptosis during nutritional stress. However, we...... found recently that ChpAK (MazF) does not confer cell killing but rather, induces a bacteriostatic condition from which the cells could be resuscitated. Results presented here yield a mechanistic explanation for the detrimental effect on cell growth exerted by ChpAK and the homologous ChpBK protein of E......AK cleaved tmRNA in its coding region. Thus, ChpAK and ChpBK inhibit translation by a mechanism very similar to that of E. coli RelE. On the basis of these results, we propose a model that integrates TA loci into general prokaryotic stress physiology....

  14. Marketingová situační analýza BK Lions Jindřichův Hradec

    OpenAIRE

    Hron, Patrik

    2014-01-01

    The aim of this bachelor thesis is to carry out an analysis of each part of marketing mix and the environment in which the BK Lions Jindřichův Hradec basketball club is situated. Suitable and applicable recommendations are suggested to make the marketing mix more effective based on analysis of marketing situation. The first part is focused on describing the marketing mix and marketing situational analysis at a theoretical level. The practical part describes particular marketing tools which ar...

  15. Coinfection with Epstein–Barr Virus (EBV), Human Papilloma Virus (HPV) and Polyoma BK Virus (BKPyV) in Laryngeal, Oropharyngeal and Oral Cavity Cancer

    OpenAIRE

    Drop, Bartłomiej; Strycharz-Dudziak, Małgorzata; Kliszczewska, Ewa; Polz-Dacewicz, Małgorzata

    2017-01-01

    Most research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma (SCC) development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. The aim of this study was to analyse the prevalence of co-infection with human papillomavirus (HPV), Epstein–Barr virus (EBV) and polyoma BK virus (BKPyV) in oral, oropharyngeal and laryngeal squamous cell carcinomas in Polish patients. The correlations between...

  16. Mechanism of the modulation of BK potassium channel complexes with different auxiliary subunit compositions by the omega-3 fatty acid DHA.

    Science.gov (United States)

    Hoshi, Toshinori; Tian, Yutao; Xu, Rong; Heinemann, Stefan H; Hou, Shangwei

    2013-03-19

    Large-conductance Ca(2+)- and voltage-activated K(+) (BK) channels are well known for their functional versatility, which is bestowed in part by their rich modulatory repertoire. We recently showed that long-chain omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA) found in oily fish lower blood pressure by activating vascular BK channels made of Slo1+β1 subunits. Here we examined the action of DHA on BK channels with different auxiliary subunit compositions. Neuronal Slo1+β4 channels were just as well activated by DHA as vascular Slo1+β1 channels. In contrast, the stimulatory effect of DHA was much smaller in Slo1+β2, Slo1+LRRC26 (γ1), and Slo1 channels without auxiliary subunits. Mutagenesis of β1, β2, and β4 showed that the large effect of DHA in Slo1+β1 and Slo1+β4 is conferred by the presence of two residues, one in the N terminus and the other in the first transmembrane segment of the β1 and β4 subunits. Transfer of this amino acid pair from β1 or β4 to β2 introduces a large response to DHA in Slo1+β2. The presence of a pair of oppositely charged residues at the aforementioned positions in β subunits is associated with a large response to DHA. The Slo1 auxiliary subunits are expressed in a highly tissue-dependent fashion. Thus, the subunit composition-dependent stimulation by DHA demonstrates that BK channels are effectors of omega-3 fatty acids with marked tissue specificity.

  17. Surface deformation and friction characteristic of nano scratch at ductile-removal regime for optical glass BK7.

    Science.gov (United States)

    Li, Chen; Zhang, Feihu; Ding, Ye; Liu, Lifei

    2016-08-20

    Nano scratch for optical glass BK7 based on the ductile-removal regime was carried out, and the influence rule of scratch parameters on surface deformation and friction characteristic was analyzed. Experimental results showed that, with increase of normal force, the deformation of burrs in the edge of the scratch was more obvious, and with increase of the scratch velocity, the deformation of micro-fracture and burrs in the edge of the scratch was more obvious similarly. The residual depth of the scratch was measured by atomic force microscope. The experimental results also showed that, with increase of normal force, the residual depth of the scratch increased linearly while the elastic recovery rate decreased. Furthermore, with increase of scratch velocity, the residual depth of the scratch decreased while the elastic recovery rate increased. The scratch process of the Berkovich indenter was divided into the cutting process of many large negative rake faces based on the improved cutting model, and the friction characteristic of the Berkovich indenter and the workpiece was analyzed. The analysis showed that the coefficient of friction increased and then tended to be stable with the increase of normal force. Meanwhile, the coefficient of friction decreased with the increase of scratch velocity, and the coefficients, k ln(v) and μ0, were introduced to improve the original formula of friction coefficient.

  18. Clinical polyomavirus BK variants with agnogene deletion are non-functional but rescued by trans-complementation

    International Nuclear Information System (INIS)

    Myhre, Marit Renee; Olsen, Gunn-Hege; Gosert, Rainer; Hirsch, Hans H.; Rinaldo, Christine Hanssen

    2010-01-01

    High-level replication of polyomavirus BK (BKV) in kidney transplant recipients is associated with the emergence of BKV variants with rearranged (rr) non-coding control region (NCCR) increasing viral early gene expression and cytopathology. Cloning and sequencing revealed the presence of a BKV quasispecies which included non-functional variants when assayed in a recombinant virus assay. Here we report that the rr-NCCR of BKV variants RH-3 and RH-12, both bearing a NCCR deletion including the 5' end of the agnoprotein coding sequence, mediated early and late viral reporter gene expression in kidney cells. However, in a recombinant virus they failed to produce infectious progeny despite large T-antigen and VP1 expression and the formation of nuclear virus-like particles. Infectious progeny was generated when the agnogene was reconstructed in cis or agnoprotein provided in trans from a co-existing BKV rr-NCCR variant. We conclude that complementation can rescue non-functional BKV variants in vitro and possibly in vivo.

  19. Phylogenetic reconstruction and polymorphism analysis of BK virus VP2 gene isolated from renal transplant recipients in China.

    Science.gov (United States)

    Wang, Zhang-Yang; Hong, Wei-Long; Zhu, Zhe-Hui; Chen, Yun-Hao; Ye, Wen-LE; Chu, Guang-Yu; Li, Jia-Lin; Chen, Bi-Cheng; Xia, Peng

    2015-11-01

    BK polyomavirus (BKV) is important pathogen for kidney transplant recipients, as it is frequently re-activated, leading to nephropathy. The aim of this study was to investigate the phylogenetic reconstruction and polymorphism of the VP2 gene in BKV isolated from Chinese kidney transplant recipients. Phylogenetic analysis was carried out in the VP2 region from 135 BKV-positive samples and 28 reference strains retrieved from GenBank. The unweighted pair-group method with arithmetic mean (UPGMA) grouped all strains into subtypes, but failed to subdivide strains into subgroups. Among the plasma and urine samples, all plasma (23/23) and 82 urine samples (82/95) were identified to contain subtype I; the other 10 urine samples contained subtype IV. A 86-bp fragment was identified as a highly conserved sequence. Following alignment with 36 published BKV sequences from China, 92 sites of polymorphism were identified, including 11 single nucleotide polymorphisms (SNPs) prevalent in Chinese individuals and 30 SNPs that were specific to the two predominant subtypes I and IV. The limitations of the VP2 gene segment in subgrouping were confirmed by phylogenetic analysis. The conserved sequence and polymorphism identified in this study may be helpful in the detection and genotyping of BKV.

  20. Conocimientos y actitudes de los estudiantes de medicina de instituciones de educación superior de Barranquillla sobre donación y trasplante de órganos. Julio-noviembre de 1999

    Directory of Open Access Journals (Sweden)

    Rafael Tuesca Molina

    2002-01-01

    Full Text Available Con el objetivo de identificar las actitudes y evaluar el conocimiento sobre donación y trasplante de órganos se realizó este estudio descriptivo transversal en la población de estudiantes de medicina de tres instituciones de educación superior de Barranquilla (Norte, Libre y San Martín tomando una muestra aleatoria de 993 participantes. La información se obtuvo mediante unas encuestas autodiligenciadas. La población de estudiantes encuestados presentó una media de 25 años y desviación estándar de 11.82 años. La razón de estudiantes por nivel (básico-clínico fue de 1.0:1. y larazóndemasculinidadfuede1,1:1.Conrespectoalconocimientoteóricoenlapoblaciónencuestada, se encontró una frecuencia modal de respuestas en el rango de regular de 69.8% y en el rango bueno de 19.7%. Al comparar el conocimiento con la institución educativa se encontró una diferencia significativa a favor de la Universidad Libre, donde el conocimiento positivo correspondió al 25.2%, con un chi cuadrado de 23.23 y un valor de p=0.0007, para 6 grados de libertad. Al comparar el conocimiento teórico entre los estudiantes del área básica con los del área clínica, estos últimos presentaron un mejor conocimiento (OR= 0.38 ICal 95% 0.27-0.53, con un chi cuadrado de 35.08 y p=0.0000. Con relación al marco legal, existe desconocimiento sobre las leyes que regulan la donaciónytrasplantedeórganos,ysepresentóuníndicededesconocimientode15:1.Lagranmayoría de los estudiantes encuestados desconoce la existencia del programa de donación y trasplante de órganos en Barranquilla (89.5%. Con respecto a la manera de obtener información sobre esta temática,el53.6%manifiestóquelahaadquiridoatravésdemediosmasivosdecomunicación,20.4% ha acudido a simposios o cursos sobre esta temática y el 12.7% ha consultado libros o revistas especializadassobreestetema. Los autores consideran ampliar la cátedra sobre donación y trasplante de órganos en los programas de

  1. Dinámicas científicas, técnicas e institucionales en el desarrollo de la diálisis y los trasplantes renales en la Argentina

    Directory of Open Access Journals (Sweden)

    Romero, Lucía

    2012-12-01

    Full Text Available This paper analyzes the origin and development of experimental and clinical research about kidney transplant and dialysis in Argentina which were carried out, from 1957, at the Instituto de Investigaciones Médicas (IIM de la Facultad de Medicina, at the Universidad de Buenos Aires (UBA. The aim is to show how these developments were possible due to the convergence of social and cognitive dynamics. The major boost given to research and clinical experience on transplants and dialysis at the IIM was because they were a a central part of the institutional commitment carried out by Alfredo Lanari, the administrative and political authority of the IIM, b a way to continue and take profit of the previous accumulation on local research capacities, human resources and knowledge on transplants and c a consequence of new scientific cooperation links settled between Lanari`s researchers and the international pioneers in this experimental and clinical field, which allowed the acquisition of technical equipment and knowledge applied in the local context. The progressive stabilization of those researches not only gave scientific prestige to the IIM but produced significant changes for Acute Kidney Failure and Chronic Kidney Failure sick people, whose treatment or cure were non-existent for them until then.

    En este trabajo se analiza la emergencia y la evolución de las investigaciones experimentales y clínicas sobre trasplantes renales y diálisis en la Argentina, llevadas a cabo, a partir de 1957, en el Instituto de Investigaciones Médicas (IIM de la Facultad de Medicina, de la Universidad de Buenos Aires (UBA. Se intenta mostrar cómo estas realizaciones fueron posibles gracias a la convergencia de dinámicas sociales y cognitivas. El impulso que recibieron las investigaciones y las prácticas sobre trasplantes y diálisis en el IIM fueron a parte central de las apuestas institucionales llevadas adelante por Alfredo Lanari, director

  2. Computed Tomography Findings of Human Polyomavirus BK (BKV)-Associated Cystitis in Allogeneic Hematopoietic Stem Cell Transplant Recipients

    International Nuclear Information System (INIS)

    Schulze, M.; Beck, R.; Igney, A.; Vogel, M.; Maksimovic, O.; Claussen, C.D.; Faul, C.; Horger, M.

    2008-01-01

    Background: Over 70% of the general population worldwide is positive for antibodies against polyomavirus hominis type 1 (BKV). Polyomavirus can be reactivated in immunocompromised patients and thereby induce urogenital tract infection, including cystitis. Purpose: To describe the computed tomography (CT) findings of human polyomavirus-induced cystitis in adult patients after allogeneic hematopoietic stem cell transplantation (allogeneic HCT). Material and Methods: The study population was a retrospective cohort of 11 consecutive adult patients (eight men, three women; age range 22-59 years, mean 42.9 years) who received allogeneic HCT between December 2003 and December 2007 and were tested positive for urinary BKV infection. All CT scans were evaluated with regard to bladder wall thickness, mucosal enhancement, distinct layering of thickened bladder wall, and presence of intravesical clots, perivesical stranding as well as attenuation values of intravesical urine. Clinical data concerning transplant and conditioning regimen variables and laboratory parameters were correlated with degree and extent of imaging findings. Results: All patients had clinical signs of cystitis with different degrees of thickening of the urinary bladder wall. Well-delineated urinary bladder layers were present in six patients. Thickening of the urinary bladder wall was continuous in nine of 11 patients. Increased attenuation of intravesical urine was found in seven patients with hemorrhagic cystitis. Four patients had intraluminal clots. Perivesical stranding was not a major CT finding, occurring in a mild fashion in three of 11 patients. The clinical classification of hemorrhagic cystitis did not correlate with the analyzed imaging parameters. Patient outcome was not influenced by this infectious complication. Conclusion: CT findings in patients with polyomavirus BK cystitis consist of different degrees of bladder wall thickening usually with good delineation of all mural layers and

  3. Computed Tomography Findings of Human Polyomavirus BK (BKV)-Associated Cystitis in Allogeneic Hematopoietic Stem Cell Transplant Recipients

    Energy Technology Data Exchange (ETDEWEB)

    Schulze, M.; Beck, R.; Igney, A.; Vogel, M.; Maksimovic, O.; Claussen, C.D.; Faul, C.; Horger, M. [Dept. of Diagnostic Radiology, Dept. of Internal Medicine-Oncology, and Inst. of Medical Virology, Eberhard-Karls Univ., Tbingen (Germany)

    2008-12-15

    Background: Over 70% of the general population worldwide is positive for antibodies against polyomavirus hominis type 1 (BKV). Polyomavirus can be reactivated in immunocompromised patients and thereby induce urogenital tract infection, including cystitis. Purpose: To describe the computed tomography (CT) findings of human polyomavirus-induced cystitis in adult patients after allogeneic hematopoietic stem cell transplantation (allogeneic HCT). Material and Methods: The study population was a retrospective cohort of 11 consecutive adult patients (eight men, three women; age range 22-59 years, mean 42.9 years) who received allogeneic HCT between December 2003 and December 2007 and were tested positive for urinary BKV infection. All CT scans were evaluated with regard to bladder wall thickness, mucosal enhancement, distinct layering of thickened bladder wall, and presence of intravesical clots, perivesical stranding as well as attenuation values of intravesical urine. Clinical data concerning transplant and conditioning regimen variables and laboratory parameters were correlated with degree and extent of imaging findings. Results: All patients had clinical signs of cystitis with different degrees of thickening of the urinary bladder wall. Well-delineated urinary bladder layers were present in six patients. Thickening of the urinary bladder wall was continuous in nine of 11 patients. Increased attenuation of intravesical urine was found in seven patients with hemorrhagic cystitis. Four patients had intraluminal clots. Perivesical stranding was not a major CT finding, occurring in a mild fashion in three of 11 patients. The clinical classification of hemorrhagic cystitis did not correlate with the analyzed imaging parameters. Patient outcome was not influenced by this infectious complication. Conclusion: CT findings in patients with polyomavirus BK cystitis consist of different degrees of bladder wall thickening usually with good delineation of all mural layers and

  4. Computed Tomography Findings of Human Polyomavirus BK (BKV)-Associated Cystitis in Allogeneic Hematopoietic Stem Cell Transplant Recipients

    Energy Technology Data Exchange (ETDEWEB)

    Schulze, M.; Beck, R.; Igney, A.; Vogel, M.; Maksimovic, O.; Claussen, C.D.; Faul, C.; Horger, M. (Dept. of Diagnostic Radiology, Dept. of Internal Medicine-Oncology, and Inst. of Medical Virology, Eberhard-Karls Univ., Tbingen (Germany))

    2008-12-15

    Background: Over 70% of the general population worldwide is positive for antibodies against polyomavirus hominis type 1 (BKV). Polyomavirus can be reactivated in immunocompromised patients and thereby induce urogenital tract infection, including cystitis. Purpose: To describe the computed tomography (CT) findings of human polyomavirus-induced cystitis in adult patients after allogeneic hematopoietic stem cell transplantation (allogeneic HCT). Material and Methods: The study population was a retrospective cohort of 11 consecutive adult patients (eight men, three women; age range 22-59 years, mean 42.9 years) who received allogeneic HCT between December 2003 and December 2007 and were tested positive for urinary BKV infection. All CT scans were evaluated with regard to bladder wall thickness, mucosal enhancement, distinct layering of thickened bladder wall, and presence of intravesical clots, perivesical stranding as well as attenuation values of intravesical urine. Clinical data concerning transplant and conditioning regimen variables and laboratory parameters were correlated with degree and extent of imaging findings. Results: All patients had clinical signs of cystitis with different degrees of thickening of the urinary bladder wall. Well-delineated urinary bladder layers were present in six patients. Thickening of the urinary bladder wall was continuous in nine of 11 patients. Increased attenuation of intravesical urine was found in seven patients with hemorrhagic cystitis. Four patients had intraluminal clots. Perivesical stranding was not a major CT finding, occurring in a mild fashion in three of 11 patients. The clinical classification of hemorrhagic cystitis did not correlate with the analyzed imaging parameters. Patient outcome was not influenced by this infectious complication. Conclusion: CT findings in patients with polyomavirus BK cystitis consist of different degrees of bladder wall thickening usually with good delineation of all mural layers and

  5. A combination of genistein and magnesium enhances the vasodilatory effect via an eNOS pathway and BK(Ca) current amplification.

    Science.gov (United States)

    Sun, Lina; Hou, Yunlong; Zhao, Tingting; Zhou, Shanshan; Wang, Xiaoran; Zhang, Liming; Yu, Guichun

    2015-04-01

    The phytoestrogen genistein (GST) and magnesium have been independently shown to regulate vascular tone; however, their individual vasodilatory effects are limited. The aim of this study was to examine the combined effects of GST plus magnesium on vascular tone in mesenteric arteries. The effects of pretreatment with GST (0-200 μmol/L), MgCl2 (0-4.8 mmol/L) and GST plus MgCl2 on 10 μmol/L phenylephrine (PE) precontracted mesenteric arteries in rats were assessed by measuring isometric force. BK(Ca) currents were detected by the patch clamp method. GST caused concentration- and partial endothelium-dependent relaxation. Magnesium resulted in dual adjustment of vascular tone. Magnesium-free solution eliminated the vasodilatation of GST in both endothelium-intact and denuded rings. GST (50 μmol/L) plus magnesium (4.8 mmol/L) caused stronger relaxation in both endothelium-intact and denuded rings. Pretreatment with the nitric oxide synthase (NOS) inhibitor L-N-nitroarginine methyl ester (L-NAME, 100 μmol/L) significantly inhibited the effects of GST, high magnesium, and the combination of GST and magnesium. BK(Ca) currents were amplified to a greater extent when GST (50 μmol/L) was combined with 4.8 versus 1.2 mmol/L Mg(2+). Our data suggest that GST plus magnesium provides enhanced vasodilatory effects in rat mesenteric arteries compared with that observed when either is used separately, which was related to an eNOS pathway and BK(Ca) current amplification.

  6. La Poste

    CERN Multimedia

    2004-01-01

    http://www.cern.ch/cern50/ The French Post Office has issued ten commemorative envelopes bearing original images depicting CERN and its history. This special fiftieth anniversary collector's edition will be available on the French Post Office (« La Poste ») stand at the Open Day on 16 October, in the CMS experiment hall at Cessy. Information for collectors: a pictorial postmark and date stamp have been specially designed and produced for the occasion with the assistance of CERN's graphics team. Sending a philatelic souvenir is a great way to commemorate the Open Day, so La Poste will be setting up a large post-box for that very purpose next to its stand. A perfect way to send images of CERN all around the world...

  7. Cytomegalovirus and BK-Virus co-infection of a clinically non-functioning adrenal adenoma: innocent bystanders or new pathogenetic agents?

    Science.gov (United States)

    Pomara, G; Cappello, F; Barzon, L; Morelli, G; Rappa, F; Benvegna, L; Giannarini, G; Palù, G; Selli, C

    2006-01-01

    We report a case of a 64-year-old woman who underwent left adrenalectomy with removal of a 8,5 cm clinically non-functioning adrenocortical adenoma and a 4-cm myelolipoma. Molecular testing for viral infection demonstrated the presence of cytomegalovirus (CMV) DNA sequences in the adrenal adenoma, but not in the myelolipoma (confirmed by immunohistochemistry). Moreover, the adrenal adenoma was also positive for parvovirus B19, and both adrenal tumor samples were positive for polyomavirus BK (BKV) and adenovirus DNA sequences. This is the first report of co-infection of an adrenocortical adenoma by CMV and BKV. The role of these viruses in adrenal tumorigenesis was postulated.

  8. Factores diagnósticos endoscópicos y pronósticos relacionados con el Helicobacter Pylori en la enfermedad injerto contra huésped tras el trasplante alogénico de células hematopoyéticas

    OpenAIRE

    Velasco Guardado, Antonio

    2016-01-01

    [ES] INTRODUCCIÓN: El trabajo consta de tres artículos originales relacionados por un lado con el diagnóstico endoscópico e histológico en la Enfermedad de Injerto contra Huésped (EICH) tras el trasplante alogénico de células hematopoyéticas (TACH) y por otro sobre el papel que juega la infección por Helicobacter pylori (HP) en dicha enfermedad. El tracto gastrointestinal es la principal diana de la Enfermedad de Injerto contra Huésped. Su diagnóstico se basa en los hallazgos endoscópicos e ...

  9. An in-house assay for BK polyomavirus quantification using the Abbott m2000 RealTime system.

    Science.gov (United States)

    Muldrew, Kenneth L; Lovett, Jennie L

    2013-11-01

    BK polyomavirus (BKPyV) quantification is useful for monitoring renal transplant patient response to therapy. The Abbott m2000 RealTime System employed by some clinical laboratories to perform US Food and Drug Administration-approved assays can also be used to develop in-house assays such as the one presented here. This study aimed to validate an in-house quantitative real-time PCR assay targeting the BKPyV major capsid VP1 gene for assessment of viral load using the Abbott m2000 RealTime System. BKPyV load was measured in 95 urine and plasma samples previously tested for BKPyV by one of three laboratories (46 BKPyV-positive samples consisting of 35 plasma and 11 urine samples; 49 samples negative for BKPyV consisting of 47 plasma and two urine samples). Two additional plasma specimens from the College of American Pathologists proficiency testing survey were also analysed. Precision studies were performed by diluting a high-viral-titre patient sample into BKPyV-negative pooled plasma to create high-positive (6.16 log10 copies ml(-1)) and low-positive (3.16 log10 copies ml(-1)) samples. For precision studies of inter-assay variability, a high-positive (7.0 log10 copies ml(-1)) and a low-positive (3.0 log10 copies ml(-1)) sample were measured in 20 separate runs. The assay's limit of quantification and limit of detection were 2.70 and 2.25 log10 copies ml(-1), respectively. The assay was linear from 2.70 to 9.26 log10 copies ml(-1). Of the 48 known positives, 43 were detected as positive, with three reported by the reference laboratory as values lower than the limit of detection. Two known positives at 3.27 and 3.80 log10 copies ml(-1) tested negative by the m2000 BKPyV assay. Of the 49 known negative samples, 48 were negative by the m2000 BKPyV load assay, with one sample confirmed positive by a reference laboratory. Qualitative analysis prior to discrepancy testing demonstrated a sensitivity of 89.58 % and a specificity of 97.96 %. Precision studies

  10. Monitoring post

    DEFF Research Database (Denmark)

    Hansen, Pelle Guldborg; Jespersen, Andreas Maaløe

    2013-01-01

    This blog reports on the rumour that White House considers establishing a nudge unit similar to the UK Behavioural Insights Team. Given The Nudging Networks purpose and the work of the iNudgeYou-team this post is updated continuously to follow the US debate as it unfolds. Which issues are raised...

  11. Efficient uptake of blood-borne BK and JC polyomavirus-like particles in endothelial cells of liver sinusoids and renal vasa recta.

    Directory of Open Access Journals (Sweden)

    Jaione Simon-Santamaria

    Full Text Available Liver sinusoidal endothelial cells (LSECs are specialized scavenger cells that mediate high-capacity clearance of soluble waste macromolecules and colloid material, including blood-borne adenovirus. To explore if LSECs function as a sink for other viruses in blood, we studied the fate of virus-like particles (VLPs of two ubiquitous human DNA viruses, BK and JC polyomavirus, in mice. Like complete virions, VLPs specifically bind to receptors and enter cells, but unlike complete virions, they cannot replicate. 125I-labeled VLPs were used to assess blood decay, organ-, and hepatocellular distribution of ligand, and non-labeled VLPs to examine cellular uptake by immunohisto- and -cytochemistry. BK- and JC-VLPs rapidly distributed to liver, with lesser uptake in kidney and spleen. Liver uptake was predominantly in LSECs. Blood half-life (∼1 min, and tissue distribution of JC-VLPs and two JC-VLP-mutants (L55F and S269F that lack sialic acid binding affinity, were similar, indicating involvement of non-sialic acid receptors in cellular uptake. Liver uptake was not mediated by scavenger receptors. In spleen, the VLPs localized to the red pulp marginal zone reticuloendothelium, and in kidney to the endothelial lining of vasa recta segments, and the transitional epithelium of renal pelvis. Most VLP-positive vessels in renal medulla did not express PV-1/Meca 32, suggesting location to the non-fenestrated part of vasa recta. The endothelial cells of these vessels also efficiently endocytosed a scavenger receptor ligand, formaldehyde-denatured albumin, suggesting high endocytic activity compared to other renal endothelia. We conclude that LSECs very effectively cleared a large fraction of blood-borne BK- and JC-VLPs, indicating a central role of these cells in early removal of polyomavirus from the circulation. In addition, we report the novel finding that a subpopulation of endothelial cells in kidney, the main organ of polyomavirus persistence, showed

  12. Cytomegalovirus and BK-Virus co-infection of a clinically non-functioning adrenal adenoma: innocent bystanders or new pathogenetic agents?

    Directory of Open Access Journals (Sweden)

    G Pomara

    2009-06-01

    Full Text Available We report a case of a 64-year-old woman who underwent left adrenalectomy with removal of a 8,5 cm clinically non-functioning adrenocortical adenoma and a 4-cm myelolipoma. Molecular testing for viral infection demonstrated the presence of cytomegalovirus (CMV DNA sequences in the adrenal adenoma, but not in the myelolipoma (confirmed by immunohistochemistry. Moreover, the adrenal adenoma was also positive for parvovirus B19, and both adrenal tumor samples were positive for polyomavirus BK (BKV and adenovirus DNA sequences. This is the first report of co-infection of an adrenocortical adenoma by CMV and BKV. The role of these viruses in adrenal tumorigenesis was postulated.

  13. Eleven new heaviest isotopes of elements Z=105 to Z=117 identified among the products of 249Bk+48Ca reactions

    International Nuclear Information System (INIS)

    Oganessian, Yu. Ts.; Abdullin, F. Sh.; Dmitriev, S. N.; Itkis, M. G.; Lobanov, Yu. V.; Mezentsev, A. N.; Polyakov, A. N.; Sagaidak, R. N.; Shirokovsky, I. V.; Subbotin, V. G.; Sukhov, A. M.; Tsyganov, Yu. S.; Utyonkov, V. K.; Voinov, A. A.; Vostokin, G. K.; Bailey, P. D.; Benker, D. E.; Ezold, J. G.; Porter, C. E.; Riley, F. D.

    2011-01-01

    The heaviest isotopes of elements Z=117 to Z=105, 294 117, 293 117, 290 115, 289 115, 286 113, 285 113, 282 Rg, 281 Rg, 278 Mt, 274 Bh, and 270 Db, were identified by means of the Dubna gas-filled recoil separator among the products of the 249 Bk + 48 Ca reaction. The details of the observed six decay chains, indicating the production and decay of isotopes 293 117 and 294 117, are presented and discussed. The decay energies and resulting half-lives of these new nuclei show a strong rise of stability with increasing neutron number, validating the concept of the island of enhanced stability for superheavy nuclei [Oganessian et al., Phys. Rev. Lett. 104, 142502 (2010)].

  14. "Legal highs"--toxicity in the clinical and medico-legal aspect as exemplified by suicide with bk-MBDB administration.

    Science.gov (United States)

    Rojek, Sebastian; Kłys, Małgorzata; Strona, Marcin; Maciów, Martyna; Kula, Karol

    2012-10-10

    The easily available "legal highs", which are products containing psychoactive substances, such as cathinones, piperazines and synthetic cannabinoids, are abused by adolescents in Poland and in the world as alternatives to classic drugs, such as amphetamines or marijuana. The majority of these potentially dangerous substances are still legal and they are associated with a risk of severe poisoning or even death, and provide new challenges in clinical and forensic toxicological practice. Investigations in the field of "designer drugs" may be well illustrated by the case of a suicide of a 21-year old male who ingested a specified dose of a preparation called "Amphi-bi-a" that contains bk-MBDB, chemically 2-methylamino-1-(3,4-methylenedioxyphenyl) butan-1-one, which belongs to the cathinone group, as a synthetic euphoric empathogen and psychoactive stimulant that is chemically similar to MDMA. It is one of more common components of "legal highs" examined in Poland and other countries. The documentation of the case includes a clinical assessment of the patient's health status performed during his almost 4-h hospitalization before death, autopsy and histological examinations supported by toxicological findings revealing bk-MBDB at extremely high concentrations (at 20 mg/l in the blood and 33 mg/kg in the liver); hence, this body of evidence contributes to knowledge in the field of "designer drugs". Inventions of designers of new psychoactive xenobiotics, which are much in demand, especially in view of the dynamic Internet marketing, which drums up narcobusiness, must be balanced by a national strategy developed by medical, legal and educational circles in the modern civilized world in order to prevent the spreading of the phenomenon. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  15. BK Virus-Hemorrhagic Cystitis Following Allogeneic Stem Cell Transplantation: Clinical Characteristics and Utility of Leflunomide Treatment

    Directory of Open Access Journals (Sweden)

    Young Hoon Park

    2016-08-01

    Full Text Available Objective: BK virus-hemorrhagic cystitis (BKV-HC is a potential cause of morbidity and mortality in patients having undergone allogeneic stem cell transplantation (Allo-SCT. We analyzed the clinical features of BKV-HC following Allo-SCT and reported the utility of leflunomide therapy for BKV-HC. Materials and methods: From January 2005 to June 2014, among the 69 patients underwent Allo-SCT in our institution, the patients who experienced BKV-HC were investigated retrospectively. Results: Hemorrhagic cystitis (HC was observed in 30 patients (43.5%, and among them, 18 patients (26.1% were identified as BKV-HC. The median age of the patients (12 males and 6 females was 45 years (range, 13-63. Patients received Allo-SCT from acute myeloid leukemia (n=11, aplastic anemia (n=4, myelodysplastic syndrome (n=2, and non-Hodgkin lymphoma (n=1.The donor types were a HLA-matched sibling donor for 6 patients, HLA-matched unrelated donor for 9, and a haploidentical familial donor for 2. The median onset and duration of BKV-HC was on day 21 (range, 7-97 after transplantation and 22 days (range, 6-107. Eleven patients (62.1% had grade I-II HC and seven patients (38.9% had grade III-IV (high-grade HC. Among the seven patients who had high-grade HC, one had complete response (CR, one partial response (PR, and five no response (NR. Among the five non-responders, one died of BKV-HC associated complications. The remaining four patients were treated with leflunomide, with achieving CR (n=2 and PR (n=2. The median duration from the start of leflunomide therapy to response was 13 days (range, 8–17 days. All patients tolerated the leflunomide treatment well, with three patients having mild gastrointestinal symptoms, including anorexia and abdominal bloating. Conclusion: BKV-HC was commonly observed in patients with HC following Allo-SCT. In high-grade BKV-HC patients who fail supportive care, leflunomide may be a feasible option without significant toxicity. Materials

  16. Hemoterapia en el trasplante de médula ósea: Experiencia en el instituto de hematología e inmunología Hemotherapy in bone marrow transplantation: Experience at the Institute of Hematology and Immunology

    Directory of Open Access Journals (Sweden)

    María E Alfonso Valdés

    1999-08-01

    Full Text Available Se describe la hemoterapia aplicada a 19 pacientes con enfermedades hematológicas malignas que recibieron trasplante de médula ósea autólogo (n=10 y alogénico (n=9. El consumo promedio de concentrados de eritrocitos y concentrados de plaquetas fue de 6,1 y 57,3, respectivamente. El uso de ambos componentes fue mayor en los enfermos que recibieron una médula ósea alogénica. Los pacientes que recibieron tratamiento con ciclosporina requirieron un número menor de concentrados de eritrocitos y plaquetas que los que no recibieron este inmunosupresor. En 3 casos existió incompatibilidad ABO donante-receptor, por lo que se adoptaron diversas medidas para evitar la hemólisis postrasplante. En el caso con incompatibilidad mayor se eliminaron los eritrocitos de la médula ósea, se realizaron 3 plasmaféresis al receptor y se le administró una transfusión de eritrocitos ABO incompatibles. En el caso con incompatibilidad menor se separó el plasma de la médula ósea. En 1 caso con incompatibilidad de tipo mixto se combinaron las medidas de los casos anteriores. En ninguno de los casos se produjo hemólisis postrasplanteThe hemotherapy applied to 19 patients with malignant hematological diseases that underwent autologous bone marrow transplantation (n=10 and allogeneic bone marrow transplantation (n=9 is described. The average consumption of erythrocytes concentrates and of platelets concentrates was 6.1 and 57.3 respectively. The use of both component was higher among those patients that received an allogeneic bone marrow. The patients who were treated with cyclosporine required a higher number of erythrocyte and platelet concentrates than those who did not receive this immunosupressor. As ABO donor-recepient incompatibility was observed in 3 cases, some measures were taken to prevent posttransplantation hemolysis. In the case with mejor incompatibility erythrocytes were eliminated from the bone marrow, the recipient underwent

  17. Post petroleum

    International Nuclear Information System (INIS)

    Urry, John

    2014-01-01

    In this original book-object, John Urry describes a world conditioned by an all-oil system. Our movement, possessions, and even our social relationships are entirely dependent on this rapidly-depleting energy source. He shows that the 20. century created the illusion of a future that, today, proves untenable, even in the medium term. While everyone's attention is still focused on just how long this reprieve will last, John Urry shifts the debate, asking the much more fundamental question: 'What happens next?' What would a world without oil look like? How would our lives as producers and consumers play out in a world deprived of this resource? In spite of growing awareness of the depletion of oil reserves, these questions remain unanswered. That is why the author alerts us to the need to prepare for post-oil now, and asks: without political awareness, how can our societies radically reinvent their relationship to energy and movement? John Urry thus imagines what will become of 'disconnected' societies in the form of four 'post-oil' scenarios, and calls us to action. His work is of particular importance to the Mobile Lives Forum, which aims to imagine good mobile lives for the future and prepare for the mobility transition. This text is transcript of a lecture given by John Urry to the Mobile Lives Forum's Scientific Steering Committee. Table of contents: 1 - Illusion: Understanding the energization of a social phenomenon; The birth of a new socio-technical system; United states: founders of an all-energy society; The emergence of an oil-based civilisation; Increasing difficulties in the use of oil; The role of oil in the current economic crisis; Can oil-dependency be deleted?; 2 - 4 scenarios: The Magic bullet future; digital lives; Unplugging: a post-car society; Warlordism in a world of shortages; 3 - Conclusion

  18. SUSPICIOUS POST

    CERN Multimedia

    Relations with the Host States Service

    2001-01-01

    If you receive a suspicious letter or package in the post, please do not open it and contact the Fire Brigade Tel. 74444. They will contact the CERN Medical Service. This is especially important in today's current situation with regard to the Anthrax scare. For further information, the Permanent Mission of Switzerland to the International Organisations in Geneva recommends the following web sites:   http://www.admin.ch/ch/f/cf/brennpunkt/03.html http://www.who.int/emc-documents/zoonoses/whoemczdi986c.html

  19. Fatores de risco cardiovascular em pacientes pediátricos após um ano de transplante renal Factores de riesgo cardiovascular en pacientes pediátricos después de un año de trasplante renal Cardiovascular risk factors in pediatric patients after one year of renal transplant

    Directory of Open Access Journals (Sweden)

    Líndia Kalliana da Costa Araújo Alves Carvalho

    2010-01-01

    Full Text Available OBJETIVO: Identificar a frequência de diabetes mellitus e a presença de fatores de risco cardiovascular em pacientes pediátricos após um ano de transplante renal. MÉTODOS: Estudo retrospectivo, de cunho documental e exploratório, realizado de janeiro de 2000 a janeiro de 2006, abrangendo 111 prontuários de pacientes pediátricos (0 a 18 anos incompletos submetidos a transplante renal no Hospital do Rim e Hipertensão e no Hospital São Paulo da Universidade Federal de São Paulo. RESULTADOS: Foram analisados 111 pacientes, 50,5% utilizavam anti-hipertensivos antes do transplante renal. Um ano após este número caiu para 28%. No pré-transplante 13,5% pacientes apresentaram sobrepeso e após um ano não houve alteração importante (12,6%. O número de pacientes obesos aumentou 50% após um ano de transplante renal. Aproximadamente 1% das crianças desenvolveram diabetes mellitus pós-tranplante renal. CONCLUSÃO: A presença de excesso de peso (sobrepeso e obesidade, hipertensão arterial e diabetes mellitus são freqüentes em pacientes pediátricos pós-tranplante renal.OBJETIVO: Identificar la frecuencia de diabetes mellitus y la presencia de factores de riesgo cardiovascular en pacientes pediátricos después de un año de trasplante renal. MÉTODOS: Estudio retrospectivo, de orden documental y exploratorio, realizado de enero de 2000 a enero de 2006, abarcando 111 fichas de pacientes pediátricos (0 a 18 años incompletos sometidos a trasplante renal en el Hospital del Riñón e Hipertensión y en el Hospital Sao Paulo de la Universidad Federal de Sao Paulo. RESULTADOS: Fueron analizados 111 pacientes, 50,5% utilizaban anti-hipertensores antes del trasplante renal. Un año después este número cayó para 28%. En el pre-trasplante, 13,5% pacientes presentaron sobrepeso y después de un año no hubo alteración importante (12,6%. El número de pacientes obesos aumentó 50% después de un año de trasplante renal. Aproximadamente 1

  20. Posting system

    International Nuclear Information System (INIS)

    Hackney, S.

    1983-01-01

    A system for posting hazardous materials into and out of an enclosure, such as a glovebox, through a port in a wall of the enclosure. The port is normally closed by a door which cooperates with a removable end closure, on a container or the like when the latter is presented to and secured at the port. The container is secured in position at the port by means of a rotatable coupling ring. A single interlock ensures that the door cannot be opened in the absence of a container at the port and also that the container cannot be removed from the port when the door is open. In place of the container, a glove secured to a rigid sleeve may be used to enable the operator to perform a work function within the glovebox. (author)

  1. Posting system

    International Nuclear Information System (INIS)

    Jones, E.L.

    1983-01-01

    A posting system for the movement of equipment, such as a manipulator, into and out of an enclosure e.g. a cell or glovebox, for toxic or radioactive materials has the manipulator arranged within a collapsible bellows-like container with an end of the container cooperating with a port entry to the enclosure. The collapsible container isolates the manipulator from the environment outside the enclosure and allows the manipulator to enter and leave the contaminated enclosure without breach of the containment. A particular construction of cell for use with radioactive material is described, having a thick wall of shielding material such as concrete provided with a door normally closed by a Pb shutter and having a cylindrical gamma shield block located over the shutter on the exterior of the wall. (author)

  2. ASAI ISOLAT BAKTERI KITINOLITIK BACILLUS SP. BK17 PADA MEDIA PEMBAWA TANAH GAMBUT DAN KOMPOS JANJANG KELAPA SAWIT DALAM MENGHAMBAT PERTUMBUHAN JAMUR PATOGEN SCLEROTIUM ROLFSII DAN FUSARIUM OXYSPORUM PADA KECAMBAH CABAI

    Directory of Open Access Journals (Sweden)

    Deswidya Hutauruk

    2016-10-01

    Full Text Available Assay of chitinolytic bacterial isolate of Bacillus sp. Bk17 in peat and palm oil bunch compost as carrier media in inhibiting Sclerotium rolfsii and Fusarium oxysporum of chilli seedlings. Sclerotium rolfsii and Fusarium oxysporum have been known as causal agents of seedling-off of chilli. Biological control has been used as an alternative control to replace chemical control. This study was aimed to determine the viability and ability of chitinolityc bacteria Bacillus sp. BK17 in carrier media of peat and palm oil bunch compost and in growing media to control seedling-off caused by S. rolfsii dan F. oxysporum of chilli. Our previous study showed that Bacillus sp. BK17 could reduce disease severity and intensity. Bacterial viability was measured as colony number grown after 90 days of storage in minimum salt medium with colloidal chitin as sole C source. Reduction of disease infection was measured as seedling number infected by S. rolfsii dan F. oxysporum. Seedling performances were measured as seedling height, leaf number and dry-weight after 30-days of growth. The result showed that bacterial cell viability was still high in both peat and palm oil bunch compost both with and without colloidal chitin addition after 90 days of storage. It was also shown that during application bacterial cell could grow. Seedling performaces i.e. seedling height, leaf number and dry-weight showed to be normal or even increase compared to those of pathogenic fungal inoculation only and (- control.

  3. El criterio inmunológico de selección para el trasplante renal: Un aspecto científico-ético Inmunologic choice criterion for renal transplantation: a scientific-ethical issue

    Directory of Open Access Journals (Sweden)

    Roberto Álvarez Hidalgo

    2004-08-01

    Full Text Available La insuficiencia renal crónica se considera una de las enfermedades crónicas no trasmisibles de mayor incidencia (1 / 1000, por lo que constituye un problema de salud en el mundo. En la actualidad es el transplante renal el tratamiento de elección, el cual representa una innovación tecnológica en el contexto de la Revolución Científico técnica, que ha alcanzado niveles altos de eficacia. Uno de los factores que intervienen en el éxito es la selección inmunológica de la pareja donante-receptor en base a los antígenos principales de histocompatibilidad (HLA. Esto asegura la supervivencia del injerto y el paciente a largo plazo. Los dilemas éticos son frecuentes en la donación de órganos tanto en el caso del donante vivo, como en el del cadáver. En la práctica diaria se realizan preguntas tales como: ¿En qué formas distribuir los órganos de donantes cadáver?, ó ¿El xenotrasplante y el trasplante de donante vivo no relacionado son alternativas válidas? Por tanto se pueden hacer ante la escasez de órganos?. Se han diseñado normas para resolver situaciones que exijan la toma de decisiones. Una correcta selección inmunológica sobre bases científicas y éticas asegura la calidad de vida del trasplantado y soluciona su problema de saludChronic renal failure is considered one of the non-transmissible chronic illnesses of higher incidence (1 / 1000, constituting a health problem all over the world. Nowadays, it is renal transplantation the treatment of choice, which represents a technological innovation in the context of the Scientific-Technological Revolution that has reached high levels of effectiveness. One of the factors that intervene in the success is the immunologic selection of the donor-receiver couple based on the main antigens of histocompatibility (HLA. This assures the implant and patient's long term survival. The ethical dilemmas are frequent in the donation of organs either in the case of alive or death donors

  4. In vitro inactivation of Chlamydia trachomatis and of a panel of DNA (HSV-2, CMV, adenovirus, BK virus) and RNA (RSV, enterovirus) viruses by the spermicide benzalkonium chloride.

    Science.gov (United States)

    Bélec, L; Tevi-Benissan, C; Bianchi, A; Cotigny, S; Beumont-Mauviel, M; Si-Mohamed, A; Malkin, J E

    2000-11-01

    Kinetics of inactivation by the detergent spermicide benzalkonium chloride (BZK) of Chlamydia trachomatis and of a panel of DNA viruses [herpes simplex virus hominis type 2 (HSV-2), cytomegalovirus (CMV), adenovirus (ADV) and BK virus (BKV)] and RNA [respiratory syncytial virus (RSV) and enterovirus (ENV)] were established in accordance with a standardized in vitro protocol. After a 5 min incubation, inactivation of >95% of HSV-2 and CMV was obtained at a concentration of 0.0025% (w/v) (25 Ig/L); concentrations as low as 0.0005%, 0.0050% and 0.0125%, induced a 3.0 log10 reduction in infectivity of HSV-2 and CMV, RSV and ADV, respectively. After a 60 min incubation, concentrations of 0.0125% and 0.050% provided a 3.0 log10 reduction in infectivity of ENV and BKV, respectively. These features indicate that sensitivity to BZK was very high (HSV-2 and CMV) or high (RSV) for enveloped viruses, intermediate (ADV) or low (ENV and BKV) for non-enveloped viruses. Furthermore, BZK had marked antichlamydial activity, showing >99% killing after only a 1 min incubation at a concentration of 0.00125%. BZK demonstrates potent in vitro activity against the majority of microorganisms causing sexually transmitted infectious diseases, including those acting as major genital cofactors of human immunodeficiency virus transmission. These attributes qualify BZK as a particularly attractive candidate for microbicide development.

  5. Analytical characterization of three cathinone derivatives, 4-MPD, 4F-PHP and bk-EPDP, purchased as bulk powder from online vendors.

    Science.gov (United States)

    Apirakkan, Orapan; Frinculescu, Anca; Shine, Trevor; Parkin, Mark C; Cilibrizzi, Agostino; Frascione, Nunzianda; Abbate, Vincenzo

    2018-02-01

    Novel emerging drugs of abuse, also referred as new psychoactive substances, constitute an ever-changing mixture of chemical compounds designed to circumvent legislative controls by means of chemical modifications of previously banned recreational drugs. One such class, synthetic cathinones, namely β-keto derivatives of amphetamines, has been largely abused over the past decade. A number of new synthetic cathinones are detected each year, either in bulk powders/crystals or in biological matrices. It is therefore important to continuously monitor the supply of new synthetic derivatives and promptly report them. By using complementary analytical techniques (i.e. one- and two-dimensional NMR, FT-IR, GC-MS, HRMS and HPLC-UV), this study investigates the detection, identification and full characterization of 1-(4-methylphenyl)-2-(methylamino)pentanone (4-methylpentedrone, 4-MPD), 1-(4-fluorophenyl)-2-(pyrrolidin-1-yl)hexanone (4F-PHP) and 1-(1,3-benzodioxol-5-yl)-2-(ethylamino)-1-pentanone (bk-EPDP), three emerging cathinone derivatives. Copyright © 2017 John Wiley & Sons, Ltd.

  6. Rhodiolae Kirliowii Radix et Rhizoma and Crataegus pinnatifida Fructus Extracts Effectively Inhibit BK Virus and JC Virus Infection of Host Cells

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    San-Yuan Chen

    2017-01-01

    Full Text Available The human polyomaviruses BK (BKPyV and JC (JCPyV are ubiquitous pathogens long associated with severe disease in immunocompromised individuals. BKPyV causes polyomavirus-associated nephropathy and hemorrhagic cystitis, whereas JCPyV is the causative agent of the fatal demyelinating disease progressive multifocal leukoencephalopathy. No effective therapies targeting these viruses are currently available. The goal of this study was to identify Chinese medicinal herbs with antiviral activity against BKPyV and JCPyV. We screened extracts of Chinese medicinal herbs for the ability to inhibit hemagglutination by BKPyV and JCPyV virus-like particles (VLPs and the ability to inhibit BKPyV and JCPyV binding and infection of host cells. Two of the 40 herbal extracts screened, Rhodiolae Kirliowii Radix et Rhizoma and Crataegus pinnatifida Fructus, had hemagglutination inhibition activity on BKPyV and JCPyV VLPs and further inhibited infection of the cells by BKPyV and JCPyV, as evidenced by reduced expression of viral proteins in BKPyV-infected and JCPyV-infected cells after treatment with Rhodiolae Kirliowii Radix et Rhizoma or Crataegus pinnatifida Fructus extract. The results in this work show that both Rhodiolae Kirliowii Radix et Rhizoma and Crataegus pinnatifida Fructus may be sources of potential antiviral compounds for treating BKPyV and JCPyV infections.

  7. Hydrophobic interaction between contiguous residues in the S6 transmembrane segment acts as a stimuli integration node in the BK channel

    Science.gov (United States)

    Carrasquel-Ursulaez, Willy; Contreras, Gustavo F.; Sepúlveda, Romina V.; Aguayo, Daniel; González-Nilo, Fernando

    2015-01-01

    Large-conductance Ca2+- and voltage-activated K+ channel (BK) open probability is enhanced by depolarization, increasing Ca2+ concentration, or both. These stimuli activate modular voltage and Ca2+ sensors that are allosterically coupled to channel gating. Here, we report a point mutation of a phenylalanine (F380A) in the S6 transmembrane helix that, in the absence of internal Ca2+, profoundly hinders channel opening while showing only minor effects on the voltage sensor active–resting equilibrium. Interpretation of these results using an allosteric model suggests that the F380A mutation greatly increases the free energy difference between open and closed states and uncouples Ca2+ binding from voltage sensor activation and voltage sensor activation from channel opening. However, the presence of a bulky and more hydrophobic amino acid in the F380 position (F380W) increases the intrinsic open–closed equilibrium, weakening the coupling between both sensors with the pore domain. Based on these functional experiments and molecular dynamics simulations, we propose that F380 interacts with another S6 hydrophobic residue (L377) in contiguous subunits. This pair forms a hydrophobic ring important in determining the open–closed equilibrium and, like an integration node, participates in the communication between sensors and between the sensors and pore. Moreover, because of its effects on open probabilities, the F380A mutant can be used for detailed voltage sensor experiments in the presence of permeant cations. PMID:25548136

  8. ECIL guidelines for the prevention, diagnosis and treatment of BK polyomavirus-associated haemorrhagic cystitis in haematopoietic stem cell transplant recipients.

    Science.gov (United States)

    Cesaro, Simone; Dalianis, Tina; Hanssen Rinaldo, Christine; Koskenvuo, Minna; Pegoraro, Anna; Einsele, Hermann; Cordonnier, Catherine; Hirsch, Hans H

    2018-01-01

    To define guidelines for BK polyomavirus (BKPyV)-associated haemorrhagic cystitis (BKPyV-HC) after paediatric and adult HSCT. Review of English literature and evidence-based recommendations by expert consensus. BKPyV-HC occurs in 8%-25% of paediatric and 7%-54% of adult recipients undergoing allogeneic HSCT. Diagnosis requires the triad of cystitis, macro-haematuria and high urine BKPyV loads >7 log10 copies/mL, and exclusion of other relevant aetiologies. BKPyV viraemia is frequent and may serve as a more specific semiquantitative follow-up marker. No randomized controlled trials are available to inform antiviral prophylaxis or treatment. However, hyper-hydration and/or bladder irrigation showed limited prophylactic value. Fluoroquinolones are not effective for prophylaxis or treatment, but rather increase antibiotic resistance. Hyperbaric oxygen or fibrin glue is marginally effective based on small case series from correspondingly equipped centres. Although cidofovir has been reported to improve and/or reduce BKPyV viraemia or viruria, the current data do not support its regular use. BKPyV-HC remains a disabling unmet clinical need in HSCT that requires novel approaches supported by proper clinical trials. © The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  9. 48Ca+249Bk fusion reaction leading to element Z = 117: long-lived α-decaying 270Db and discovery of 266Lr.

    Science.gov (United States)

    Khuyagbaatar, J; Yakushev, A; Düllmann, Ch E; Ackermann, D; Andersson, L-L; Asai, M; Block, M; Boll, R A; Brand, H; Cox, D M; Dasgupta, M; Derkx, X; Di Nitto, A; Eberhardt, K; Even, J; Evers, M; Fahlander, C; Forsberg, U; Gates, J M; Gharibyan, N; Golubev, P; Gregorich, K E; Hamilton, J H; Hartmann, W; Herzberg, R-D; Heßberger, F P; Hinde, D J; Hoffmann, J; Hollinger, R; Hübner, A; Jäger, E; Kindler, B; Kratz, J V; Krier, J; Kurz, N; Laatiaoui, M; Lahiri, S; Lang, R; Lommel, B; Maiti, M; Miernik, K; Minami, S; Mistry, A; Mokry, C; Nitsche, H; Omtvedt, J P; Pang, G K; Papadakis, P; Renisch, D; Roberto, J; Rudolph, D; Runke, J; Rykaczewski, K P; Sarmiento, L G; Schädel, M; Schausten, B; Semchenkov, A; Shaughnessy, D A; Steinegger, P; Steiner, J; Tereshatov, E E; Thörle-Pospiech, P; Tinschert, K; Torres De Heidenreich, T; Trautmann, N; Türler, A; Uusitalo, J; Ward, D E; Wegrzecki, M; Wiehl, N; Van Cleve, S M; Yakusheva, V

    2014-05-02

    The superheavy element with atomic number Z=117 was produced as an evaporation residue in the (48)Ca+(249)Bk fusion reaction at the gas-filled recoil separator TASCA at GSI Darmstadt, Germany. The radioactive decay of evaporation residues and their α-decay products was studied using a detection setup that allowed measuring decays of single atomic nuclei with half-lives between sub-μs and a few days. Two decay chains comprising seven α decays and a spontaneous fission each were identified and are assigned to the isotope (294)117 and its decay products. A hitherto unknown α-decay branch in (270)Db (Z = 105) was observed, which populated the new isotope (266)Lr (Z = 103). The identification of the long-lived (T(1/2) = 1.0(-0.4)(+1.9) h) α-emitter (270)Db marks an important step towards the observation of even more long-lived nuclei of superheavy elements located on an "island of stability."

  10. Prevalence of Polyoma BK Virus (BKPyV), Epstein-Barr Virus (EBV) and Human Papilloma Virus (HPV) in Oropharyngeal Cancer.

    Science.gov (United States)

    Polz-Gruszka, Dorota; Morshed, Kamal; Jarzyński, Adrian; Polz-Dacewicz, Małgorzata

    2015-01-01

    The aim of this study was to analyze the prevalence of BK virus, Human Papillomavirus and Epstein-Barr virus in oropharyngeal cancer, and to test our hypothesis that BKV/HPV/EBV co-infection plays a role in oropharyngeal squamous cell carcinoma. The correlation between viral infection, OSCC, anatomic location, pre-treatment staging, evidence of metastases to lymph nodes, and grading was also investigated. The examination samples were collected from 62 patients from paraffin tissue blocks. Males (90.3%) with, smoking (83.9%) and alcohol abuse (67.7%) problems prevailed in the studied group. G2 histological type was recognized in 80.6% cases. T4 (77.4%) and N2 (56.5%) traits occurred in the majority of patients. No cases of metastasis were observed (M0 100%). HPV - 24.2%, EBV - 27.4% and BKV 17.7% were detected in the studied samples. We observed co-infection EBV/BKV in 8% of cases, HPV/BKV in 4.8%, and HPV/EBV in 9% cases. Only in two cases co-infection of all three viruses was found.

  11. Antigen-Specificity of T Cell Infiltrates in Biopsies With T Cell-Mediated Rejection and BK Polyomavirus Viremia: Analysis by Next Generation Sequencing.

    Science.gov (United States)

    Zeng, G; Huang, Y; Huang, Y; Lyu, Z; Lesniak, D; Randhawa, P

    2016-11-01

    This study interrogates the antigen-specificity of inflammatory infiltrates in renal biopsies with BK polyomavirus (BKPyV) viremia (BKPyVM) with or without allograft nephropathy (BKPyVN). Peripheral blood mononuclear cells (PBMC) from five healthy HLA-A0101 subjects were stimulated by peptides derived from the BKPYV proteome or polymorphic regions of HLA. Next generation sequencing of the T cell-receptor complementary DNA was performed on peptide-stimulated PBMC and 23 biopsies with T cell-mediated rejection (TCMR) or BKPyVN. Biopsies from patients with BKPyVM or BKVPyVN contained 7.7732 times more alloreactive than virus-reactive clones. Biopsies with TCMR also contained BKPyV-specific clones, presumably a manifestation of heterologous immunity. The mean cumulative T cell clonal frequency was 0.1378 for alloreactive clones and 0.0375 for BKPyV-reactive clones. Samples with BKPyVN and TCMR clustered separately in dendrograms of V-family and J-gene utilization patterns. Dendrograms also revealed that V-gene, J-gene, and D-gene usage patterns were a function of HLA type. In conclusion, biopsies with BKPyVN contain abundant allospecific clones that exceed the number of virus-reactive clones. The T cell component of tissue injury in viral nephropathy appears to be mediated primarily by an "innocent bystander" mechanism in which the principal element is secondary T cell influx triggered by both antiviral and anti-HLA immunity. © Copyright 2016 The American Society of Transplantation and the American Society of Transplant Surgeons.

  12. Differential Patterns of Large Tumor Antigen-Specific Immune Responsiveness in Patients with BK Polyomavirus-Positive Prostate Cancer or Benign Prostatic Hyperplasia

    Science.gov (United States)

    Sais, Giovanni; Wyler, Stephen; Hudolin, Tvrtko; Banzola, Irina; Mengus, Chantal; Bubendorf, Lukas; Wild, Peter J.; Hirsch, Hans H.; Sulser, Tullio; Spagnoli, Giulio C.

    2012-01-01

    The role of the polyomavirus BK (BKV) large tumor antigen (L-Tag) as a target of immune response in patients with prostate cancer (PCa) has not been investigated thus far. In this study, we comparatively analyzed humoral and cellular L-Tag-specific responsiveness in age-matched patients bearing PCa or benign prostatic hyperplasia, expressing or not expressing BKV L-Tag-specific sequences in their tissue specimens, and in non-age-matched healthy individuals. Furthermore, results from patients with PCa were correlated to 5-year follow-up clinical data focusing on evidence of biochemical recurrence (BR) after surgery (prostate specific antigen level of ≥0.2 ng/ml). In peripheral blood mononuclear cells (PBMC) from patients with PCa with evidence of BR and BKV L-Tag-positive tumors, stimulation with peptides derived from the BKV L-Tag but not those derived from Epstein-Barr virus, influenza virus, or cytomegalovirus induced a peculiar cytokine gene expression profile, characterized by high expression of interleukin-10 (IL-10) and transforming growth factor β1 and low expression of gamma interferon genes. This pattern was confirmed by protein secretion data and correlated with high levels of anti-BKV L-Tag IgG. Furthermore, in PBMC from these PCa-bearing patients, L-Tag-derived peptides significantly expanded an IL-10-secreting CD4+ CD25+(high) CD127− FoxP3+ T cell population with an effector memory phenotype (CD103+) capable of inhibiting proliferation of autologous anti-CD3/CD28-triggered CD4+ CD25− T cells. Collectively, our findings indicate that potentially tolerogenic features of L-Tag-specific immune response are significantly associated with tumor progression in patients with BKV+ PCa. PMID:22647697

  13. Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression

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    Michela Cioni

    2013-01-01

    Full Text Available Background. Among human polyomaviruses, only BK virus (BKV and JC virus (JCV encode an agnoprotein upstream of VP1 on the viral late transcript. BKV agnoprotein is abundantly expressed late in the viral life cycle, but specific cellular and humoral immune responses are low or absent. We hypothesized that agnoprotein might contribute to BKV immune evasion by downregulating HLA expression, similar to Herpes simplex virus-1 ICP47. Methods UTA-6 or primary human renal proximal tubular epithelial cells (RPTEC were co-transfected with plasmids constitutively expressing agnoprotein, or ICP47, and enhanced green-fluorescent protein (EGFP. EGFP-gated cells were analyzed for HLA-ABC and HLA-DR expression by flow cytometry. HLA-ABC and HLA-DR expression was also analyzed on UTA-6 bearing tetracycline-regulated agnoprotein or ICP47. Effects of agnoprotein on viral peptide-dependent T-cell killing were investigated using 51Cr release. Results. ICP47 downregulated HLA-ABC without affecting HLA-DR, whereas agnoprotein did not affect HLA-ABC or HLA-DR expression. Interferon-γ treatment increased HLA-ABC in a dose-dependent manner, which was antagonized by ICP47, but not by agnoprotein. In UTA-6 cells, agnoprotein expression did neither impair HLA-ABC or -DR expression nor peptide-specific killing impaired by HLA-matched T-cells. Conclusion. Unlike the HSV-1 ICP47, BKV agnoprotein does not contribute to viral immune evasion by down-regulating HLA-ABC, or interfere with HLA-DR expression or peptide-dependent T-cell cytotoxicity.

  14. Impact of HMG-CoA reductase inhibitors on the incidence of polyomavirus-associated nephropathy in renal transplant recipients with human BK polyomavirus viremia.

    Science.gov (United States)

    Gabardi, S; Ramasamy, S; Kim, M; Klasek, R; Carter, D; Mackenzie, M R; Chandraker, A; Tan, C S

    2015-08-01

    Up to 20% of renal transplant recipients (RTR) will develop human BK polyomavirus (BKPyV) viremia. BKPyV viremia is a pre-requisite of polyomavirus-associated nephropathy (PyVAN). Risk of BKPyV infections increases with immunosuppression. Currently, the only effective therapy against PyVAN is reductions in immunosuppression, but this may increase the risk of rejection. In vitro data have shown that pravastatin dramatically decreased caveolin-1 expression in human renal proximal tubular epithelial cells (HRPTEC) and suppressed BKPyV infection in these cells. Based on these data, we postulated that statin therapy may prevent the progression of BKPyV viremia to PyVAN. A multicenter, retrospective study was conducted in adult RTR transplanted between July 2005 and March 2012. All patients with documented BKPyV viremia (viral load >500 copies/mL on 2 consecutive tests) were included. Group I consisted of patients taking a statin before the BKPyV viremia diagnosis (n = 32), and Group II had no statin exposure before or after the BKPyV viremia diagnosis (n = 36). The primary endpoint was the incidence of PyVAN. Demographic data, transplant characteristics, and the degree of immunosuppression (i.e., induction/maintenance therapies, rejection treatment) were similar between the groups, with the exception of more diabetics in Group I. The incidence of PyVAN was comparable between the 2 groups (Group I = 28.1% vs. Group II = 41.7%; P = 0.312). Despite the proven in vitro effectiveness of pravastatin preventing BKPyV infection in HRPTEC, statins at doses maximized for cholesterol lowering, in RTR with BKPyV viremia, did not prevent progression to PyVAN. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  15. Coinfection with Epstein–Barr Virus (EBV, Human Papilloma Virus (HPV and Polyoma BK Virus (BKPyV in Laryngeal, Oropharyngeal and Oral Cavity Cancer

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    Bartłomiej Drop

    2017-12-01

    Full Text Available Most research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma (SCC development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. The aim of this study was to analyse the prevalence of co-infection with human papillomavirus (HPV, Epstein–Barr virus (EBV and polyoma BK virus (BKPyV in oral, oropharyngeal and laryngeal squamous cell carcinomas in Polish patients. The correlations between viral infection, SCC, demographic parameters, evidence of metastases and grading were also investigated. Fresh-frozen tumour tissue samples were collected from 146 patients with laryngeal, oropharyngeal and oral cancer. After DNA extraction, the DNA of the studied viruses was detected using polymerase chain rection (PCR assay. Males (87.7% with a history of smoking (70.6% and alcohol abuse (59.6% prevailed in the studied group. Histological type G2 was recognized in 64.4% cases. The patients were most frequently diagnosed with T2 stage (36.3% and with N1 stage (45.8%. Infection with at least two viruses was detected in 56.2% of patients. In this group, co-infection with HPV/EBV was identified in 34.1% of cases, EBV/BKV in 23.2%, HPV/BKV in 22.0%, and HPV/EBV/BKV in 20.7%. No difference of multiple infection in different locations of cancer was observed. The prevalence of poorly differentiated tumours (G3 was more frequent in co-infection with all three viruses than EBV or BKV alone. A significant correlation was observed between tumour dimensions (T and lymph-node involvement (N in co-infected patients compared to single infection. Further studies are necessary to clarify whether co-infection plays an important role in the initiation and/or progression of oncogenic transformation of oral, oropharyngeal and laryngeal epithelial cells.

  16. Coinfection with Epstein-Barr Virus (EBV), Human Papilloma Virus (HPV) and Polyoma BK Virus (BKPyV) in Laryngeal, Oropharyngeal and Oral Cavity Cancer.

    Science.gov (United States)

    Drop, Bartłomiej; Strycharz-Dudziak, Małgorzata; Kliszczewska, Ewa; Polz-Dacewicz, Małgorzata

    2017-12-19

    Most research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma (SCC) development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. The aim of this study was to analyse the prevalence of co-infection with human papillomavirus (HPV), Epstein-Barr virus (EBV) and polyoma BK virus (BKPyV) in oral, oropharyngeal and laryngeal squamous cell carcinomas in Polish patients. The correlations between viral infection, SCC, demographic parameters, evidence of metastases and grading were also investigated. Fresh-frozen tumour tissue samples were collected from 146 patients with laryngeal, oropharyngeal and oral cancer. After DNA extraction, the DNA of the studied viruses was detected using polymerase chain rection (PCR) assay. Males (87.7%) with a history of smoking (70.6%) and alcohol abuse (59.6%) prevailed in the studied group. Histological type G2 was recognized in 64.4% cases. The patients were most frequently diagnosed with T2 stage (36.3%) and with N1 stage (45.8%). Infection with at least two viruses was detected in 56.2% of patients. In this group, co-infection with HPV/EBV was identified in 34.1% of cases, EBV/BKV in 23.2%, HPV/BKV in 22.0%, and HPV/EBV/BKV in 20.7%. No difference of multiple infection in different locations of cancer was observed. The prevalence of poorly differentiated tumours (G3) was more frequent in co-infection with all three viruses than EBV or BKV alone. A significant correlation was observed between tumour dimensions (T) and lymph-node involvement (N) in co-infected patients compared to single infection. Further studies are necessary to clarify whether co-infection plays an important role in the initiation and/or progression of oncogenic transformation of oral, oropharyngeal and laryngeal epithelial cells.

  17. Coinfection with Epstein–Barr Virus (EBV), Human Papilloma Virus (HPV) and Polyoma BK Virus (BKPyV) in Laryngeal, Oropharyngeal and Oral Cavity Cancer

    Science.gov (United States)

    Drop, Bartłomiej; Strycharz-Dudziak, Małgorzata; Kliszczewska, Ewa; Polz-Dacewicz, Małgorzata

    2017-01-01

    Most research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma (SCC) development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. The aim of this study was to analyse the prevalence of co-infection with human papillomavirus (HPV), Epstein–Barr virus (EBV) and polyoma BK virus (BKPyV) in oral, oropharyngeal and laryngeal squamous cell carcinomas in Polish patients. The correlations between viral infection, SCC, demographic parameters, evidence of metastases and grading were also investigated. Fresh-frozen tumour tissue samples were collected from 146 patients with laryngeal, oropharyngeal and oral cancer. After DNA extraction, the DNA of the studied viruses was detected using polymerase chain rection (PCR) assay. Males (87.7%) with a history of smoking (70.6%) and alcohol abuse (59.6%) prevailed in the studied group. Histological type G2 was recognized in 64.4% cases. The patients were most frequently diagnosed with T2 stage (36.3%) and with N1 stage (45.8%). Infection with at least two viruses was detected in 56.2% of patients. In this group, co-infection with HPV/EBV was identified in 34.1% of cases, EBV/BKV in 23.2%, HPV/BKV in 22.0%, and HPV/EBV/BKV in 20.7%. No difference of multiple infection in different locations of cancer was observed. The prevalence of poorly differentiated tumours (G3) was more frequent in co-infection with all three viruses than EBV or BKV alone. A significant correlation was observed between tumour dimensions (T) and lymph-node involvement (N) in co-infected patients compared to single infection. Further studies are necessary to clarify whether co-infection plays an important role in the initiation and/or progression of oncogenic transformation of oral, oropharyngeal and laryngeal epithelial cells. PMID:29257122

  18. Resultados de una encuesta sobre donación y trasplante de órganos en la zona metropolitana de Guadalajara, México Survey results regarding organ donation and transplant in metropolitan Guadalajara, Mexico

    Directory of Open Access Journals (Sweden)

    Luz Consuelo Zepeda-Romero

    2003-01-01

    Full Text Available OBJETIVO: Evaluar el conocimiento y la disposición de la donación de órganos y tejidos con fines de trasplante entre la población adulta del área metropolitana de Guadalajara, Jalisco, México. MATERIAL Y MÉTODOS: Se realizó una encuesta aplicando un cuestionario de 29 reactivos a personas mayores de 18 años de edad, mediante entrevista directa en la vía pública. La zona metropolitana se dividió en cuatro municipios, asignándose a cada uno de éstos el número de encuestas en proporción al número de habitantes, estrato socioeconómico, edad y género. Se calcularon frecuencias y ji2 para diferencias de proporciones. RESULTADOS: Se encuestaron 400 individuos. El 80.4% conocía acerca de la donación de órganos y 65% sabía que está permitido por la ley; sin embargo, solamente 24% había comentado el tema en familia. El 71% de los encuestados desconocía cómo donar sus órganos y sólo 25% sabía de la existencia de la tarjeta de donación. Por otra parte, 66% estarían dispuestos a donarlos, 16.5% no lo harían y 17% estaban indecisos. La mutilación corporal fue la razón principal (40% para no donar. El 89% estarían dispuestos a autorizar la extracción de órganos de un familiar, siempre que éste hubiera manifestado su deseo en vida; de lo contrario, sólo 29% procederían con la autorización. Finalmente, 65% consideró la licencia de conducir como un medio adecuado de expresar el deseo de donar y 50% considera insuficiente la información que sobre el tema se ha difundido por los medios de comunicación. CONCLUSIONES: La mayoría de la población de la zona metropolitana de la ciudad de Guadalajara tiene conocimiento sobre la donación de órganos y estaría dispuesta a donarlos. Sin embargo, un alto porcentaje desconoce el cómo hacerlo y considera insuficiente la información difundida sobre el tema. Una campaña permanente a través de los medios de comunicación masiva podría solucionar lo anterior e incrementaría la

  19. Stem-cells transplantation for myocardial regeneration Trasplante autólogo de médula ósea: propuesta basada en la evidencia, para un consenso en enfermedad cardiovascular

    Directory of Open Access Journals (Sweden)

    Francisco Cuellar Ambrosi

    2005-04-01

    éneo que se desarrolla después de un "evento índice", el cual produce daño del tejido muscular cardíaco por la pérdida de cardiomiocitos o por la alteración de la capacidad del miocardio para generar una fuerza eficiente para la contracción ventricular. La noxa inicial puede ser de aparición súbita como en el síndrome coronario agudo o insidiosa y gradual como en la enfermedad coronaria crónica y las cardiomiopatías primarias y secundarias. Independiente del factor que genere el síndrome, se produce una serie de respuestas hemodinámicas y neurohormonales con cambios moleculares complejos, que conducen a la progresión de la disfunción ventricular y a mayor pérdida de células contráctiles funcionantes por necrosis o por apoptosis. Las estrategias terapéuticas desarrolladas para interrumpir este círculo vicioso de disfunción miocárdica y remodelación ventricular, cambian las condiciones en que se produce el trabajo miocárdico, con la consecuente mejoría de la enfermedad y de la tasa de supervivencia. Sin embargo, este enfoque terapéutico, conocido como la "aproximación Jurásica", no logra recuperar el tejido lesionado durante el proceso, lo que permite la progresión de la enfermedad hasta llegar a la falla cardíaca terminal y la muerte. Estudios experimentales y clínicos han demostrado que la implantación de células progenitoras derivadas de la médula ósea producen angiogénesis y recuperación de la función cardíaca en pacientes con cardiopatía isquémica aguda y crónica. Con esta terapia se ha demostrado mejoría significativa de la sintomatología, de la capacidad funcional, del estado funcional, del tamaño del defecto de perfusión miocárdica medido por pruebas de medicina nuclear y de la fracción de eyección al igual que disminución del volumen sistólico final del ventrículo izquierdo, sin complicaciones relacionadas con el procedimiento. En conclusión, el trasplante de células progenitoras derivadas de la médula ósea es

  20. Alterações fisiológicas da morte encefálica em potenciais doadores de órgãos e tecidos para transplantes Los cambios fisiológicos de la muerte cerebral en potenciales donadores de órganos y tejidos para trasplante Physiological changes of brain death in potential donors of organs and tissues for transplantation

    Directory of Open Access Journals (Sweden)

    Sarah Gabriel Freire

    2012-12-01

    Full Text Available Objetivou-se descrever as alterações fisiológicas da morte encefálica em potenciais doadores de órgãos e tecidos para transplantes. Estudo exploratório descritivo com dados prospectivos e abordagem quantitativa realizado nas unidades de emergência e terapia intensiva adulto de um hospital de Pernambuco no período de abril a outubro de 2011. A população constou de 32 potenciais doadores de órgãos e tecidos para transplantes. Após aprovação do Comitê de Ética em Pesquisa, os dados foram coletados, tabulados e analisados pela estatística descritiva pelo software SPSS 15.0 e apresentados em forma de tabelas. As alterações fisiológicas foram: hipotensão arterial (100%, hipotermia (75,0%, hipernatremia (62,5%, diabetes insipidus (37,5%, hiperglicemia (32,3%, infecção (25,0%, hipertensão arterial (9,4% e úlcera de córnea (3,1%. Acredita-se que o conhecimento dessas alterações possibilita à equipe de saúde direcionar o cuidado ao potencial doador segundo as suas necessidades e, assim, manter o órgão/tecido viável para transplante.El objetivo del estudio fue describir los cambios fisiológicos de la muerte cerebral en los potenciales donadores de órganos y tejidos para trasplante. Investigación exploratoria, descriptiva, con datos prospectivos y enfoque cuantitativo, hecho en las unidades de emergencia y de cuidados intensivos de adultos de un hospital de Pernambuco en el periodo de abril a octubre de 2011. La población fue formada por 32 potenciales donadores de órganos y tejidos para trasplante. Después de la aprobación en el Comité Ético de Investigación, los datos han sido recogidos, tabulados y analizados mediante estadística descriptiva por el programa informático SPSS 15.0, siendo presentados en forma de tablas. Los cambios fisiológicos fueron: hipotensión (100%, hipotermia (75,0%, hipernatremia (62,5%, diabetes insípida (37,5%, infección (25,0%, hipertensión arterial (9,4% y la úlcera de c

  1. Alchemy: A web 2.0 real-time quality assurance platform for human immunodeficiency Virus, hepatitis C Virus, and BK Virus quantitation assays

    Directory of Open Access Journals (Sweden)

    Emmanuel Agosto-Arroyo

    2017-01-01

    Full Text Available Background: The molecular diagnostics laboratory faces the challenge of improving test turnaround time (TAT. Low and consistent TATs are of great clinical and regulatory importance, especially for molecular virology tests. Laboratory information systems (LISs contain all the data elements necessary to do accurate quality assurance (QA reporting of TAT and other measures, but these reports are in most cases still performed manually: a time-consuming and error-prone task. The aim of this study was to develop a web-based real-time QA platform that would automate QA reporting in the molecular diagnostics laboratory at our institution, and minimize the time expended in preparing these reports. Methods: Using a standard Linux, Nginx, MariaDB, PHP stack virtual machine running atop a Dell Precision 5810, we designed and built a web-based QA platform, code-named Alchemy. Data files pulled periodically from the LIS in comma-separated value format were used to autogenerate QA reports for the human immunodeficiency virus (HIV quantitation, hepatitis C virus (HCV quantitation, and BK virus (BKV quantitation. Alchemy allowed the user to select a specific timeframe to be analyzed and calculated key QA statistics in real-time, including the average TAT in days, tests falling outside the expected TAT ranges, and test result ranges. Results: Before implementing Alchemy, reporting QA for the HIV, HCV, and BKV quantitation assays took 45–60 min of personnel time per test every month. With Alchemy, that time has decreased to 15 min total per month. Alchemy allowed the user to select specific periods of time and analyzed the TAT data in-depth without the need of extensive manual calculations. Conclusions: Alchemy has significantly decreased the time and the human error associated with QA report generation in our molecular diagnostics laboratory. Other tests will be added to this web-based platform in future updates. This effort shows the utility of informatician

  2. Alchemy: A Web 2.0 Real-time Quality Assurance Platform for Human Immunodeficiency Virus, Hepatitis C Virus, and BK Virus Quantitation Assays.

    Science.gov (United States)

    Agosto-Arroyo, Emmanuel; Coshatt, Gina M; Winokur, Thomas S; Harada, Shuko; Park, Seung L

    2017-01-01

    The molecular diagnostics laboratory faces the challenge of improving test turnaround time (TAT). Low and consistent TATs are of great clinical and regulatory importance, especially for molecular virology tests. Laboratory information systems (LISs) contain all the data elements necessary to do accurate quality assurance (QA) reporting of TAT and other measures, but these reports are in most cases still performed manually: a time-consuming and error-prone task. The aim of this study was to develop a web-based real-time QA platform that would automate QA reporting in the molecular diagnostics laboratory at our institution, and minimize the time expended in preparing these reports. Using a standard Linux, Nginx, MariaDB, PHP stack virtual machine running atop a Dell Precision 5810, we designed and built a web-based QA platform, code-named Alchemy. Data files pulled periodically from the LIS in comma-separated value format were used to autogenerate QA reports for the human immunodeficiency virus (HIV) quantitation, hepatitis C virus (HCV) quantitation, and BK virus (BKV) quantitation. Alchemy allowed the user to select a specific timeframe to be analyzed and calculated key QA statistics in real-time, including the average TAT in days, tests falling outside the expected TAT ranges, and test result ranges. Before implementing Alchemy, reporting QA for the HIV, HCV, and BKV quantitation assays took 45-60 min of personnel time per test every month. With Alchemy, that time has decreased to 15 min total per month. Alchemy allowed the user to select specific periods of time and analyzed the TAT data in-depth without the need of extensive manual calculations. Alchemy has significantly decreased the time and the human error associated with QA report generation in our molecular diagnostics laboratory. Other tests will be added to this web-based platform in future updates. This effort shows the utility of informatician-supervised resident/fellow programming projects as learning

  3. Necessidades de informação de candidatos ao transplante de fígado: o primeiro passo do processo ensino-aprendizagem Necesidades de información de los candidatos a trasplante hepático: el primer paso del proceso de enseñanza-aprendizaje Information needs of liver transplant candidates: the first step of the teaching-learning process

    Directory of Open Access Journals (Sweden)

    Karina Dal Sasso Mendes

    2012-12-01

    Full Text Available A necessidade de informação é definida como a deficiência de informação ou habilidade relacionada a um domínio de vida relevante para o paciente. O objetivo do presente estudo foi identificar as necessidades de informação de candidatos em fila de espera para o transplante de fígado. Trata-se de estudo descritivo, conduzido em centro transplantador brasileiro do interior paulista. A amostra foi constituída de 55 pacientes, e a coleta de dados foi realizada nos meses de março a junho de 2009. Os resultados evidenciaram que as necessidades de informação do período pré-operatório foram as que obtiveram pontuações médias maiores. O conhecimento de informações que o candidato ao transplante de fígado precisa é relevante para o planejamento do processo ensino-aprendizagem.La necesidad de información se define como la carencia de información o habilidades relacionadas con un dominio de la vida relevante para el paciente. El objetivo de este estudio fue identificar las necesidades de información de candidatos en lista de espera para el trasplante de hígado. El diseño del estudio es de una investigación descriptiva, llevado a cabo en un centro de trasplante en São Paulo - Brasil. La muestra abarcó a 55 pacientes y los datos fueron recolectados entre marzo y junio del 2009. Los resultados mostraron mayor puntuación promedio para las necesidades de información del periodo preoperatorio. El conocimiento de informaciones que el candidato a trasplante de hígado necesita es importante para planificar el proceso de enseñanza-aprendizaje.'Information need' is defined as a deficiency of information or skill related to a domain of life that is relevant to the patient. This study's objective was to identify the information needs of candidates on the waiting list for a liver transplant. This is a descriptive study and was conducted at a transplant center in the State of São Paulo - Brazil. The sample consisted of 55 patients and

  4. Ancillary personnel faced with living liver donation in a Spanish hospital with a transplant program El personal no sanitario de un hospital español con programa de trasplantes ante la donación de vivo hepática

    Directory of Open Access Journals (Sweden)

    A. Ríos

    2006-12-01

    preparación ni formación sanitaria en la mayoría de casos, lo cual hace que su actitud pueda estar basada en el desconocimiento o miedos no fundados. El objetivo es analizar la actitud hacia la donación hepática de vivo de los profesionales no sanitarios de un hospital con programa de trasplante de órganos de cadáver y de donante vivo hepático, y analizar las variables que influyen en dicha actitud. Pacientes y método: muestra aleatoria y estratificada por servicio (n = 401 entre el personal no sanitario del hospital. La actitud se valoró mediante una encuesta validada en nuestro medio. Para su distribución se contactó en cada servicio con el representante del mismo al cual se le explicó el estudio y fue el responsable del reparto del cuestionario en los turnos seleccionados. La encuesta fue cumplimentada de forma anónima y autoadministrada. Estadística: test de Chi cuadrado, t de Student y análisis de regresión logística. Resultados: el grado de cumplimentación del cuestionario fue del 94% (n = 377. De los encuestados, el 20% (n = 74 está a favor de la donación de vivo de un hemi-hígado tanto relacionada como no relacionada, porcentaje que asciende hasta el 82% (n = 307 si la donación fuera relacionada. Del resto, el 8% (n = 30 no acepta dicha donación y el 11% (n = 40 restante está indeciso. Al analizar las variables que se relacionan con la actitud hacia la donación de vivo hepática se obtienen: la actitud hacia la donación de cadáver (p = 0,002, el creer el encuestado en la posibilidad de necesitar un trasplante en el futuro (p < 0,001 y en dicho caso la aceptación de un hígado de donante vivo (p < 0,001. Al realizar el análisis multivariante persisten ambas: a el creer el encuestado en la posibilidad de necesitar un trasplante en un futuro (OR = 1,5; y b en dicho caso la aceptación de un riñón de donante vivo (OR = 16,2. Conclusiones: la actitud hacia la donación de vivo hepática entre el personal no sanitario de un hospital

  5. Transplante cardíaco: complicações apresentadas por pacientes durante a internação Trasplante de corazón: complicaciones que presentan los pacientes durante la hospitalización Heart Transplantation: complications presented by patients during the hospitalization

    Directory of Open Access Journals (Sweden)

    Elaine Araújo da Silva

    2012-12-01

    Full Text Available Com o avanço da ciência, a insuficiência cardíaca tem como possibilidade de intervenção o transplante cardíaco. Este trabalho teve como objetivos traçar o perfil demográfico dos pacientes submetidos ao transplante cardíaco e identificar as complicações apresentadas durante a internação. Trata-se de um estudo descritivo, quantitativo, realizado com 44 prontuários de pacientes submetidos a transplante de junho/2006 a julho/2009 em um Hospital Universitário de Belo Horizonte, Minas Gerais, Brasil. O projeto atendeu às recomendações da Resolução 196/96. Pelos resultados, a maioria dos pacientes era do sexo masculino, casada, escolaridade de nível fundamental e idade média de 45,2 anos. A miocardiopatia chagásica foi a principal causa de insuficiência cardíaca. As complicações predominantes foram alteração da glicemia, insuficiência renal e infecções. A taxa de mortalidade na internação foi de 20,4%. Além das complicações biológicas só foram registradas ansiedade e depressão. São necessárias pesquisas sobre o tema nos âmbitos psicossociais e espirituais, contribuindo para o desenvolvimento científico.Hoy día, con los avances de la medicina y de la tecnología para el tratamiento de la insuficiencia cardiaca irreversible, se ha utilizado como última intervención el trasplante cardíaco. Este trabajo se desarrolló con los objetivos de delinear el perfil demográfico de los pacientes sometidos a trasplante cardíaco e identificar las complicaciones presentadas por ellos después del trasplante de corazón. Estudio descriptivo y cuantitativo, realizado en conjunto con los registros de 44 pacientes sometidos a trasplante de corazón desde Jun/2006 hasta Jul/2009, en un Hospital Universitario de Belo Horizonte, Minas Gerais, Brasil. El proyecto cumplió con las recomendaciones de la Resolución 196/96. Los resultados mostraron que la mayoría de los pacientes eran del sexo masculino, casados, con educaci

  6. Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01

    Directory of Open Access Journals (Sweden)

    Sigoillot Jean-Claude

    2009-11-01

    Full Text Available Abstract Background Mannans are key components of lignocellulose present in the hemicellulosic fraction of plant primary cell walls. Mannan endo-1,4-β-mannosidases (1,4-β-D-mannanases catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of β-mannans. Biodegradation of β-mannans by the action of thermostable mannan endo-1,4-β-mannosidase offers significant technical advantages in biotechnological industrial applications, i.e. delignification of kraft pulps or the pretreatment of lignocellulosic biomass rich in mannan for the production of second generation biofuels, as well as for applications in oil and gas well stimulation, extraction of vegetable oils and coffee beans, and the production of value-added products such as prebiotic manno-oligosaccharides (MOS. Results A gene encoding mannan endo-1,4-β-mannosidase or 1,4-β-D-mannan mannanohydrolase (E.C. 3.2.1.78, commonly termed β-mannanase, from Aspergillus niger BK01, which belongs to glycosyl hydrolase family 5 (GH5, was cloned and successfully expressed heterologously (up to 243 μg of active recombinant protein per mL in Pichia pastoris. The enzyme was secreted by P. pastoris and could be collected from the culture supernatant. The purified enzyme appeared glycosylated as a single band on SDS-PAGE with a molecular mass of approximately 53 kDa. The recombinant β-mannanase is highly thermostable with a half-life time of approximately 56 h at 70°C and pH 4.0. The optimal temperature (10-min assay and pH value for activity are 80°C and pH 4.5, respectively. The enzyme is not only active towards structurally different mannans but also exhibits low activity towards birchwood xylan. Apparent Km values of the enzyme for konjac glucomannan (low viscosity, locust bean gum galactomannan, carob galactomannan (low viscosity, and 1,4-β-D-mannan (from carob are 0.6 mg mL-1, 2.0 mg mL-1, 2.2 mg mL-1 and 1.5 mg mL-1, respectively, while the kcat values for these

  7. Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01.

    Science.gov (United States)

    Do, Bien-Cuong; Dang, Thi-Thu; Berrin, Jean-Guy; Haltrich, Dietmar; To, Kim-Anh; Sigoillot, Jean-Claude; Yamabhai, Montarop

    2009-11-13

    Mannans are key components of lignocellulose present in the hemicellulosic fraction of plant primary cell walls. Mannan endo-1,4-beta-mannosidases (1,4-beta-D-mannanases) catalyze the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of beta-mannans. Biodegradation of beta-mannans by the action of thermostable mannan endo-1,4-beta-mannosidase offers significant technical advantages in biotechnological industrial applications, i.e. delignification of kraft pulps or the pretreatment of lignocellulosic biomass rich in mannan for the production of second generation biofuels, as well as for applications in oil and gas well stimulation, extraction of vegetable oils and coffee beans, and the production of value-added products such as prebiotic manno-oligosaccharides (MOS). A gene encoding mannan endo-1,4-beta-mannosidase or 1,4-beta-D-mannan mannanohydrolase (E.C. 3.2.1.78), commonly termed beta-mannanase, from Aspergillus niger BK01, which belongs to glycosyl hydrolase family 5 (GH5), was cloned and successfully expressed heterologously (up to 243 microg of active recombinant protein per mL) in Pichia pastoris. The enzyme was secreted by P. pastoris and could be collected from the culture supernatant. The purified enzyme appeared glycosylated as a single band on SDS-PAGE with a molecular mass of approximately 53 kDa. The recombinant beta-mannanase is highly thermostable with a half-life time of approximately 56 h at 70 degrees C and pH 4.0. The optimal temperature (10-min assay) and pH value for activity are 80 degrees C and pH 4.5, respectively. The enzyme is not only active towards structurally different mannans but also exhibits low activity towards birchwood xylan. Apparent Km values of the enzyme for konjac glucomannan (low viscosity), locust bean gum galactomannan, carob galactomannan (low viscosity), and 1,4-beta-D-mannan (from carob) are 0.6 mg mL-1, 2.0 mg mL-1, 2.2 mg mL-1 and 1.5 mg mL-1, respectively, while the kcat values for these

  8. Post-traumatic Stress Disorder Post Partum

    Science.gov (United States)

    Schwab, W.; Marth, C.; Bergant, A. M.

    2012-01-01

    Traumatic birth experiences may lead to serious psychological impairment. Recent studies show that a considerable number of women can develop post-traumatic stress disorder (PTSD), in some cases in a subsyndromal form. Until now, the possibility that postpartum psychological symptoms might be a continuum of a pre-existing disorder in pregnancy has rarely been considered. This study therefore aimed to evaluate the proportion of women who develop post-traumatic stress disorder as a result of childbirth. Materials and Methods: 56 multiparous women were recruited for the study. The diagnosis of PTSD was made according to the criteria for psychological disorders in the DSM-IV (Diagnostics and Statistical Manual of Mental Disorders). The data were collected in structured interviews in the 30th to 38th week of gestation and in the 6th week post partum. Results: Of the 56 women participating, 52 (93 %) completed the survey. Uncontrolled results showed that 21.15 % of the multiparous women met the full diagnostic PTSD criteria in the 6th week post partum. After the exclusion of all cases already characterised by all criteria or a subsyndromal form of PTSD caused by previous traumatisation, the PTSD rate was below 8 % at 6 weeks postpartum (= incidence rate of PTSD post partum). Conclusions: The present study is the first prospective longitudinal study to demonstrate the occurrence of full criteria PTSD in multiparous women as a result of childbirth after having excluded pre-existing PTSD. The results of our study show a high prevalence rate of PTSD during pregnancy. A number of women report all aspects of post-traumatic stress disorder as a result of childbirth. PMID:25253905

  9. Post cardiac injury syndrome

    DEFF Research Database (Denmark)

    Nielsen, S L; Nielsen, F E

    1991-01-01

    The post-pericardiotomy syndrome is a symptom complex which is similar in many respects to the post-myocardial infarction syndrome and these are summarized under the diagnosis of the Post Cardiac Injury Syndrome (PCIS). This condition, which is observed most frequently after open heart surgery, i...... on the coronary vessels, with cardiac tamponade and chronic pericardial exudate. In the lighter cases, PCIS may be treated with NSAID and, in the more severe cases, with systemic glucocorticoid which has a prompt effect....

  10. Estudio retrospectivo de la aplicación de la amplificación génica cualitativa en muestras biológicas para el seguimiento de la toxoplasmosis en pacientes pediátricos receptores de trasplante de células progenitoras hematopoyéticas

    Directory of Open Access Journals (Sweden)

    Mónica G Nigro

    Full Text Available La toxoplasmosis es una infección oportunista causada por el parásito Toxoplasma gondii; su infección es grave y de difícil diagnóstico en pacientes que reciben un trasplante alogénico de células progenitoras hematopoyéticas (TCPH. En el Hospital de Pediatría S.A.M.I.C. "Profesor Dr. Juan P. Garrahan"� se realizó la vigilancia postrasplante de 12 pacientes receptores de TCPH mediante la técnica de PCR cualitativa. La necesidad de seguimiento de estos pacientes fue definida por el antecedente de serología positiva para toxoplasmosis en el donante o receptor y ante la imposibilidad de iniciar el uso profiláctico de trimetoprima-sulfametoxazol a causa de la condición hematológica. Dos pacientes presentaron signos de enfermedad por T. gondii con resultado de PCR positivo y recibieron tratamiento con pirimetamina-clindamicina. En otros dos, la toxoplasmosis fue causa de muerte y hallazgo de autopsia, con resultado de PCR negativo. Cuatro pacientes recibieron tratamiento contra toxoplasmosis por la detección de una PCR positiva, sin manifestaciones clínicas. En los cuatro pacientes restantes no se detectaron signos de enfermedad por toxoplasmosis, con resultados de PCR negativos durante el seguimiento. La técnica de PCR cualitativa demostró ser útil para detectar la reactivación de la toxoplasmosis en receptores de TCPH, pero tiene limitaciones para el seguimiento y la toma de decisiones clínicas en pacientes con PCR positiva que persiste en el tiempo y manifestaciones de toxicidad por el tratamiento.

  11. Post-Polio Syndrome

    Science.gov (United States)

    ... You are here Home » Disorders » All Disorders Post-Polio Syndrome Information Page Post-Polio Syndrome Information Page What research is being done? ... behavior of motor neurons many years after a polio attack. Others are looking at the mechanisms of ...

  12. Glove box posting system

    International Nuclear Information System (INIS)

    McIntosh, A.E.

    1981-01-01

    A system for posting objects into closed containers, such as glove boxes, is described in which the bag used, preferably made of plastic, does not have to be fitted and sealed by the operator during each posting operation. (U.K.)

  13. Post-quantum cryptography.

    Science.gov (United States)

    Bernstein, Daniel J; Lange, Tanja

    2017-09-13

    Cryptography is essential for the security of online communication, cars and implanted medical devices. However, many commonly used cryptosystems will be completely broken once large quantum computers exist. Post-quantum cryptography is cryptography under the assumption that the attacker has a large quantum computer; post-quantum cryptosystems strive to remain secure even in this scenario. This relatively young research area has seen some successes in identifying mathematical operations for which quantum algorithms offer little advantage in speed, and then building cryptographic systems around those. The central challenge in post-quantum cryptography is to meet demands for cryptographic usability and flexibility without sacrificing confidence.

  14. Post-quantum cryptography

    Science.gov (United States)

    Bernstein, Daniel J.; Lange, Tanja

    2017-09-01

    Cryptography is essential for the security of online communication, cars and implanted medical devices. However, many commonly used cryptosystems will be completely broken once large quantum computers exist. Post-quantum cryptography is cryptography under the assumption that the attacker has a large quantum computer; post-quantum cryptosystems strive to remain secure even in this scenario. This relatively young research area has seen some successes in identifying mathematical operations for which quantum algorithms offer little advantage in speed, and then building cryptographic systems around those. The central challenge in post-quantum cryptography is to meet demands for cryptographic usability and flexibility without sacrificing confidence.

  15. [Post-partum thyroiditis].

    Science.gov (United States)

    Neves, Celestino; Alves, Marta; Delgado, Luís; Medina, J Luís

    2009-01-01

    In the post-partum period the immune alterations are associated with the multiple autoimmune diseases relapse. After birth, immune-tolerance variation slowly disappear, and is observed a return to a normal state - after an exacerbation period - of autoimmune reactivity, during which a great increase in T cells and autoantibodies is observed. In this period - 3 to 9 months after birth - the thyroid autoimmune disease relapses or reappears. The reactivation of the immune system in the post-partum period unchains an acute phase of celular destruction which characterizes the post-partum thyroiditis.

  16. Post-Concussion Syndrome

    Science.gov (United States)

    ... psychological factors, especially since the most common symptoms — headache, dizziness and sleep problems — are similar to those often experienced by people diagnosed with depression, anxiety or post-traumatic stress disorder. In many cases, both physiological effects of ...

  17. Teaching Post-Pornography

    Directory of Open Access Journals (Sweden)

    Tim Gregory

    2018-04-01

    Full Text Available This article introduces the term ‘post-pornography’, drawing on diverse texts from the last three decades. We propose that ‘post-pornography’ expands Porn Studies beyond its focus on explicit representations of sex. First, we outline the history of post-pornography as a concept that emerged in the sex-positive, anti-censorship and queer/feminist moment in the United States in the 1980s and 1990s and has subsequently been taken up by a diverse group of artists, activists and scholars to describe practices that both reference and attempt to move beyond pornography. We define post-pornography as characterised by three aspects—the denaturalising of sex, the de-centring of the spectator and the recognition of media and technology as inseparable from sex. We examine the history of Porn Studies in the university, including in our own faculty at UNSW Art & Design, and the singular influence of Linda Williams in defining its place and setting out its pedagogical methods. We propose post-pornography as a framework that can confront prevailing assumptions about sex and sexuality that underpin Porn Studies and its critique of pornography, and outline a set of concepts that have emerged from the development of the second- and third-year art theory course Post-Pornographic Bodies.

  18. Compression stockings significantly improve hemodynamic performance in post-thrombotic syndrome irrespective of class or length.

    Science.gov (United States)

    Lattimer, Christopher R; Azzam, Mustapha; Kalodiki, Evi; Makris, Gregory C; Geroulakos, George

    2013-07-01

    Graduated elastic compression (GEC) stockings have been demonstrated to reduce the morbidity associated with post-thrombotic syndrome. The ideal length or compression strength required to achieve this is speculative and related to physician preference and patient compliance. The aim of this study was to evaluate the hemodynamic performance of four different stockings and determine the patient's preference. Thirty-four consecutive patients (40 legs, 34 male) with post-thrombotic syndrome were tested with four different stockings (Mediven plus open toe, Bayreuth, Germany) of their size in random order: class 1 (18-21 mm Hg) and class II (23-32 mm Hg), below-knee (BK) and above-knee thigh-length (AK). The median age, Venous Clinical Severity Score, Venous Segmental Disease Score, and Villalta scale were 62 years (range, 31-81 years), 8 (range, 1-21), 5 (range, 2-10), and 10 (range, 2-22), respectively. The C of C0-6EsAs,d,pPr,o was C0 = 2, C2 = 1, C3 = 3, C4a = 12, C4b = 7, C5 = 12, C6 = 3. Obstruction and reflux was observed on duplex in 47.5% legs, with deep venous reflux alone in 45%. Air plethysmography was used to measure the venous filling index (VFI), venous volume, and time to fill 90% of the venous volume. Direct pressure measurements were obtained while lying and standing using the PicoPress device (Microlab Elettronica, Nicolò, Italy). The pressure sensor was placed underneath the test stocking 5 cm above and 2 cm posterior to the medial malleolus. At the end of the study session, patients stated their preferred stocking based on comfort. The VFI, venous volume, and time to fill 90% of the venous volume improved significantly with all types of stocking versus no compression. In class I, the VFI (mL/s) improved from a median of 4.9 (range, 1.7-16.3) without compression to 3.7 (range, 0-14) BK (24.5%) and 3.6 (range, 0.6-14.5) AK (26.5%). With class II, the corresponding improvement was to 4.0 (range, 0.3-16.2) BK (18.8%) and 3.7 (range, 0.5-14.2) AK (24

  19. Computer Security: posting and mis-posting

    CERN Multimedia

    Stefan Lueders, Computer Security Team

    2015-01-01

    This is what can happen at CERN if you don't lock your computer screen...   “Hi, I am looking for a partner either male or female to attend salsa lessons. I have a great body and enjoy rubbing it against other people on the dance floor. I would consider dinner after with the right person. If you think you can keep up with me and enjoy getting sweaty send me a reply. Stay sexy…” This is the original text of a recent posting on the CERN Market webpage. Some people might find this appealing, some people think this is funny. Personally, I couldn’t care less. But professionally, we had to follow up as this text can be perceived as inappropriate and, thus, in violation of the Terms of Usage of the CERN Market as well as the CERN Computing Rules and its annex on private usage of the CERN computing facilities. We remind you that the CERN Market is a public website that can be used by people within but also outside CERN. All posts are visible world...

  20. Enfermagem em transplante de células tronco hematopoéticas: produção científica de 1997 a 2007 El papel de la enfermería en el trasplante de células tronco hematopoyéticas: producción científica de 1997 a 2007 Nursing and hematopoietic stem cell transplantation: scientific production from 1997 to 2007

    Directory of Open Access Journals (Sweden)

    Nen Nalú Alves das Mercês

    2010-04-01

    Full Text Available OBJETIVO: Identificar as publicações de enfermagem existentes no período de 1997 a 2007 referentes à temática do transplante de medula óssea e suas principais características. MÉTODOS: Estudo bibliométrico das produções científicas indexadas nas bases de dados: BDENF, LILACS, SciElo, CEPEn e Banco de Dissertações e Teses da CAPES, utilizando os descritores: enfermagem e transplante de medula óssea no recorte temporal de 1997 a 2007 RESULTADOS: Foram incluídos 37 trabalhos em âmbito nacional, sendo 17 dissertações, 2 teses e 18 artigos publicados em periódicos brasileiros, que abordam o cuidado de enfermagem, organização e gerenciamento de Serviços de Transplante de Medula Óssea, qualidade de vida, sofrimento psíquico e produção de conhecimento. CONCLUSÕES: A produção científica, mesmo que quantitativamente pequena, está em ascensão. E está baseada em teorias, conceitos e métodos, principalmente de orientação qualitativa.OBJETIVO: Identificar las publicaciones de enfermería existentes en el período de 1997 a 2007 referentes a la temática del trasplante de médula ósea y sus principales características. MÉTODOS: Estudio bibliométrico de las producciones científicas indexadas en las bases de datos: BDENF, LILACS, SciElo, CEPEn y en el banco de disertaciones y tesis de la CAPES, utilizando los descriptores: enfermería y trasplante de medula ósea en el intervalo de 1997 a 2007 RESULTADOS: Fueron incluidos 37 trabajos del ámbito nacional, siendo 17 disertaciones, 2 tesis y 18 artículos, publicados en periódicos brasileños, que abordan el cuidado de enfermería, la organización y administración de los Servicios de Trasplante de Médula Ósea, la calidad de vida, el sufrimiento psíquico, y, la producción de conocimiento. CONCLUSIONES: La producción científica, cuantitativamente pequeña, está aumentando; ella está basada en teorías, conceptos y métodos, principalmente de orientaci

  1. Post-death cloning of endangered Jeju black cattle (Korean native cattle): fertility and serum chemistry in a cloned bull and cow and their offspring.

    Science.gov (United States)

    Kim, Eun Young; Song, Dong Hwan; Park, Min Jee; Park, Hyo Young; Lee, Seung Eun; Choi, Hyun Yong; Moon, Jeremiah Jiman; Kim, Young Hoon; Mun, Seong Ho; Oh, Chang Eon; Ko, Moon Suck; Lee, Dong Sun; Riu, Key Zung; Park, Se Pill

    2013-12-17

    To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.

  2. Doença coronária obstrutiva em hepatopatas crônicos que aguardam transplante hepático Enfermedad coronaria obstructiva en hepatópatas crónicos que aguardan trasplante hepático Obstructive coronary disease in patients with chronic liver disease awaiting liver transplantation

    Directory of Open Access Journals (Sweden)

    Moacir Fernandes de Godoy

    2011-01-01

    grado importante con consecuente aumento de riesgo o incluso contraindicación para trasplante hepático. OBJETIVOS: Analizar la contribución de los factores de riesgo clásicos para la enfermedad arterial coronaria (EAC en pacientes portadores de IHA con y sin coronariopatía obstructiva. MÉTODOS: Evaluación de los factores de riesgo para EAC en 119 pacientes, en un servicio de referencia para trasplante hepático, con las siguientes características: más de 40 años de edad, portadores de IHA y sometidos a cinecoronariografía. RESULTADOS: Coronariopatía obstructiva fue detectada en 21 (17,6% de los casos. Estos pacientes presentaban realmente niveles bajos de colesterol, siendo de 129,0±53,5mg/dL con mediana de 117,0mg/dL en los hepatópatas con coronarias normales y 135,4±51,7mg/dL con mediana de 122,0mg/dL en los hepatópatas con coronariopatía obstructiva (P=0,8215. En la regresión logística multivariada, la edad, el sexo, el índice de masa corporal, así como las presencias de diabetes, de tabaquismo y de etilismo no tuvieron significancia estadística aislada en la diferenciación entre los grupos. También no hubo asociación con la etiología de la IHA. A su vez, la hipertensión arterial se mostró relevante en la asociación con EAC (P=0,0474. CONCLUSIÓN: Sólo la hipertensión arterial fue factor de riesgo con significancia estadística para el desarrollo de EAC en pacientes con IHA aguardando trasplante hepático. Por ser un factor de riesgo cambiable, este hallazgo orienta hacia una práctica de actitudes terapéuticas en la tentativa de evitarse o retardar el desarrollo de la EAC en estos pacientes.BACKGROUND: Advanced liver failure (ALF usually presents hypocholesterolemia. Nevertheless, some patients with ALF develops major coronary obstructive diseases with consequent increased risk or contraindication to liver transplantation. OBJECTIVE: To analyze the contribution of classical risk factors for coronary artery disease (CAD in patients

  3. Post-Session Authentication

    DEFF Research Database (Denmark)

    Ahmed, Naveed; Jensen, Christian D.

    2012-01-01

    Entity authentication provides confidence in the claimed identity of a peer entity, but the manner in which this goal is achieved results in different types of authentication. An important factor in this regard is the order between authentication and the execution of the associated session....... In this paper, we consider the case of post-session authentication, where parties authenticate each other at the end of their interactive session. This use of authentication is different from session-less authentication (e.g., in RFID) and pre-session authentication (e.g., for access control.) Post......-session authentication, although a new term, is not a new concept; it is the basis of at least a few practical schemes. We, for the first time, systematically study it and present the underlying authentication model. Further, we show that an important class of problems is solvable using post-session authentication...

  4. Efecto de la edad al trasplante y de la concentración de azúcar in vitro sobre la adaptación y el crecimiento de vitroplantas de papa (Solanum tuberosum L. vars. atlantic y 1625 frito lay en invernadero.

    Directory of Open Access Journals (Sweden)

    Rafael Orozco

    2016-03-01

    Full Text Available La aclimatización y posterior crecimiento y desarrollo in vivo de plantas propagadas in vitro constituye una de las etapas culminantes del proceso de micropropagación de una especie. Esta investigación se desarrolló con el objetivo de determinar el efecto de diferentes edades al trasplante (15, 20 y 25 días de cultivo y el preacondicionamiento in vitro con tres niveles de azúcar comercial (15, 30 y 45 g L-1 sobre el crecimiento y desarrollo en invernadero de vitroplantas de papa (Solanum tuberosum L. vars. Atlantic y 1625 Frito Lay. En el primer caso se usó el medio de cultivo MS (1962 suministrado por laboratorios Gibco, a razón de 17 g L-1 y suplementado con vitaminas y aminoácidos del mismo medio. En el segundo caso se usó el medio Hyponex a razón de 2.25 g L-1 suplementado con las vitaminas y aminoácidos del medio MS. Los resultados indican que es posible trasplantar a suelo plantas de ambas variedades en cualquiera de las tres edades, sin afectar el rendimiento de minitubérculos. Sin embargo, la tasa de fotosíntesis aparente (TFA se vio favorecida al incrementarse la edad. Respecto a la influencia del preacondicionamiento in vitro con diferentes concentraciones de azúcar, las plantas de la variedad Atlantic ganaron altura, peso seco de raíz y peso fresco y seco de minitubérculos con 15 g L-1 de azúcar, mientras que en plantas de la variedad 1625, este efecto se dio en la concentración de 30 g L-1; el número de minitubérculos no fue favorecido con las concentraciones de azúcar empleadas en el medio Hyponex, pero sí en el medio MS (1962. En este último caso, la TFA sufrió una ganancia gradual al aumentar la concentración de azúcar.

  5. Laboratório de transplante celular: novo cenário de atuação do enfermeiro Laboratorio de trasplante celular: nuevo escenario de la actuación de la enfermera Cellular transplantation laboratory: a new field of action for nurses

    Directory of Open Access Journals (Sweden)

    Maria Inês Corradi

    2008-04-01

    Full Text Available O trabalho apresenta um relato de experiência sobre a atuação do enfermeiro num laboratório de transplante celular. Este Laboratório tem por objetivos realizar isolamentos de células produtoras de insulina para transplante em humanos. O enfermeiro como membro da equipe interdisciplinar participa da estruturação da área física, do delineamento dos processos de trabalho, da elaboração de protocolos e de programa de capacitação da equipe. As principais atividades realizadas pelo enfermeiro incluem o controle de contaminação, treinamento em serviço e avaliação da qualidade dos processos de trabalho desenvolvidos pela equipe interdisciplinar. Os resultados têm demonstrando a efetividade do trabalho do enfermeiro neste novo campo.El presente trabajo trata de un relato de experiencia sobre la actuación del enfermero en un laboratorio de trasplante celular. El objetivo del laboratorio es realizar asilamientos de células productoras de insulina para transplante en seres humanos. El enfermero, como parte del equipo multidisciplinar tuvo participación en la estructura de todos los procesos del trabajo: elaboración de protocolos y programa de capacitación del equipo. Las principales actuaciones del enfermero incluyen el control de contaminación, entreno en el trabajo y la evaluación de la cualidad de las investigaciones hechas por el equipo multidisciplinar. Los resultados han sido favorables, enseñando la efectividad del trabajo del enfermero en esta nueva área.This article presents the experience of a nurse at a cellular transplantation laboratory. This laboratory goal is to isolate insulin producing cells for human transplantation. The nurse, as a member of an interdisciplinary team, took part in the planning of all work processes: working procedures and team training. The main activities under the nurse responsibilities include contamination control, on-the-job training and evaluation of the quality of the procedures

  6. Post-human Viewing

    DEFF Research Database (Denmark)

    Blaagaard, Bolette

    2013-01-01

    to become part of a global cultural flow, thus calling into question the physical connection between viewer and image. This article analyses what happens to that connection when not only the image but also the physical body is mediated and challenged in post-human relations, and examines the ensuing ethical...... implications. The author takes photojournalism and, in particular, mobile phone footage as a starting point for an exploration of the (post-human) body as evidence and sign of authenticity in the modern age of digital communications and journalism....

  7. Post-duralpunktur hovedpine

    DEFF Research Database (Denmark)

    Dahl, J B; Rosenberg, J

    1990-01-01

    Post dural puncture headache is one of the major complications of spinal anaesthesia. In order to establish this diagnosis, the headache must be dependent on the position of the patient. The symptoms are probably due to leakage of cerebro-spinal fluid from the subarachnoid space to the epidural...

  8. LHC magnet support post

    CERN Multimedia

    1995-01-01

    A prototype magnet support for the Large Hadron Collider (LHC). The magnet supports have to bridge a difference in temperature of 300 degrees. Electrical connections, instrumentation and the posts on which the magnets stand are the only points where heat transfer can happen through conduction. They are all carefully designed to draw off heat progressively. The posts are made of 4 mm thick glass-fibre– epoxy composite material. Each post supports 10 000 kg of magnet and leaks just 0.1 W of heat. This piece required a long development period which started in the early ’90s and continued until the end of the decade. The wires next to the support post are wires from strain gauges, which are employed to measure the stress level in the material when the support is mechanically loaded. These supports are mechanically optimized to withstand a weight of up to 100Kn (10 tons) while being as thin as possible to minimize conduction heat to magnets. This is the reason why the stress measurement was extensively done...

  9. Post-irradiation diarrhea

    International Nuclear Information System (INIS)

    Meerwaldt, J.H.

    1984-01-01

    In radiotherapy of pelvic cancers, the X-ray dose to be delivered to the tumour is limited by the tolerance of healthy surrounding tissue. In recent years, a number of serious complications of irradiation of pelvic organs were encountered. Modern radiotherapy necessitates the acceptance of a calculated risk of complications in order to achieve a better cure rate. To calculate these risks, one has to know the radiation dose-effect relationship of normal tissues. Of the normal tissues most at risk when treating pelvic tumours only the bowel is studied. In the literature regarding post-irradiation bowel complications, severe and mild complications are often mixed. In the present investigation the author concentrated on the group of patients with relatively mild symptoms. He studied the incidence and course of post-irradiation diarrhea in 196 patients treated for carcinoma of the uterine cervix or endometrium. The aims of the present study were: 1) to determine the incidence, course and prognostic significance of post-irradiation diarrhea; 2) to assess the influence of radiotherapy factors; 3) to study the relation of bile acid metabolism to post-irradiation diarrhea; 4) to investigate whether local factors (reservoir function) were primarily responsible. (Auth.)

  10. Teaching Post Keynesian Economics

    DEFF Research Database (Denmark)

    Denne bog har gør gældende, at post keynesiansk økonomi har sit egen metodiske og didaktiske grundlag, og dens realistisk analyse er tiltrængt i den aktuelle økonomiske og finansielle krise. På et tidspunkt hvor det oprindelige indhold i Keynes 'General Theory ikke længere er til stede i de flest...

  11. Characterization of highly frequent epitope-specific CD45RA+/CCR7+/- T lymphocyte responses against p53-binding domains of the human polyomavirus BK large tumor antigen in HLA-A*0201+ BKV-seropositive donors

    Directory of Open Access Journals (Sweden)

    Zajac Paul

    2006-11-01

    Full Text Available Abstract Human polyomavirus BK (BKV has been implicated in oncogenic transformation. Its ability to replicate is determined by the binding of its large tumor antigen (LTag to products of tumor-suppressor genes regulating cell cycle, as specifically p53. We investigated CD8+ T immune responses to BKV LTag portions involved in p53 binding in HLA-A*0201+ BKV LTag experienced individuals. Peptides selected from either p53-binding region (LTag351–450 and LTag533–626 by current algorithms and capacity to bind HLA-A*0201 molecule were used to stimulate CD8+ T responses, as assessed by IFN-γ gene expression ex vivo and detected by cytotoxicity assays following in vitro culture. We observed epitope-specific immune responses in all HLA-A*0201+ BKV LTag experienced individuals tested. At least one epitope, LTag579–587; LLLIWFRPV, was naturally processed in non professional antigen presenting cells and induced cytotoxic responses with CTL precursor frequencies in the order of 1/20'000. Antigen specific CD8+ T cells were only detectable in the CD45RA+ subset, in both CCR7+ and CCR7- subpopulations. These data indicate that widespread cellular immune responses against epitopes within BKV LTag-p53 binding regions exist and question their roles in immunosurveillance against tumors possibly associated with BKV infection.

  12. Are the Polyomaviruses BK and JC Associated with Opportunistic Infections, Graft-versus-Host Disease, or Worse Outcomes in Adult Patients Receiving Their First Allogeneic Stem Cell Transplantation with Low-Dose Alemtuzumab?

    Science.gov (United States)

    Schneidewind, Laila; Neumann, Thomas; Knoll, Florian; Zimmermann, Kathrin; Smola, Sigrun; Schmidt, Christian Andreas; Krüger, William

    2017-01-01

    The association of polyomaviruses BK and JC with other opportunistic infections and graft-versus-host disease (GvHD) in allogeneic stem cell transplantation is controversially discussed. We conducted a retrospective study of 64 adult patients who received their first allogeneic stem cell transplantation between March 2010 and December 2014; the follow-up time was 2 years. Acute leukemia was the most frequent underlying disease (45.3%), and conditioning included myeloablative (67.2%) and nonmyeloablative protocols (32.8%). All patients received 10 mg of alemtuzumab on day -2 (20 mg in case of mismatch) as GvHD prophylaxis. Twenty-seven patients (41.5%) developed cytomegalovirus (CMV) reactivation. BKPyV-associated hemorrhagic cystitis was diagnosed in 10 patients (15.6%). Other opportunistic infections caused by viruses or protozoa occurred rarely (reactivation, Epstein-Barr virus reactivation, human herpes virus 6, or parvovirus B19 infection requiring treatment. There was a significant correlation of BKPyV-associated hemorrhagic cystitis with toxoplasmosis (p = 0.013). Additionally, there was a significant link of simultaneous BKPyV and JCPyV viruria with toxoplasmosis (p = 0.047). BKPyV and JCPyV were not associated with GvHD, relapse, or death. We found no association of BKPyV or JCPyV with viral infections or GvHD. Only the correlation of both polyomaviruses with toxoplasmosis was significant. This is a novel and interesting finding. © 2017 S. Karger AG, Basel.

  13. Communication from La Poste

    CERN Multimedia

    2005-01-01

    We should like to remind users that post sent to the CERN site must be correctly addressed in order to reach the right destination as quickly as possible: Correspondence (goods subject to Swiss VAT): Surname, first name CERN (Department, Building & Mailbox) Route de Meyrin 385 Case Postale 1211 Genève 23 CERN (Switzerland) Official orders (goods not subject to Swiss VAT): CERN Goods Reception Order number ( Ca, Ct, Cl....) For the attention of: (surname, first name) Route de Meyrin 385 Case Postale 1211 Genève 23 CERN (Switzerland) Please inform your suppliers of the above addresses. Last minute Swiss motorway sticker on sale at the POST OFFICE, Main Building (63 R 02) 40.- CHF

  14. Post-Session Authentication

    OpenAIRE

    Ahmed , Naveed; Jensen , Christian ,

    2012-01-01

    Part 1: Full Papers; International audience; Entity authentication provides confidence in the claimed identity of a peer entity, but the manner in which this goal is achieved results in different types of authentication. An important factor in this regard is the order between authentication and the execution of the associated session. In this paper, we consider the case of post-session authentication, where parties authenticate each other at the end of their interactive session. This use of a...

  15. Síndrome post

    OpenAIRE

    Avalos, Raquel

    2014-01-01

    ¿Qué sigue luego de que una mujer terminara su relación con el último hombre que amaba? Eso, dicen ellas, depende de cuanto duró el romance o cómo sea la psiquis de cada amante. Lo cierto es que el escenario post convierte a algunas en depresivas, caletas, indiferentes y hasta amnésicas. ¿Qué te parece, varón?

  16. Pionerserien - Jytte Post

    DEFF Research Database (Denmark)

    Holck, Ulla

    2008-01-01

    Ethvert ungt fag hviler på en række pionerers arbejde med at bane vejen, udbrede kendskabet, overbevise politikere og embedsmænd, forsøge sig frem, støde panden mod en mur og forsøge igen. Hele tiden drevet af troen, lysten og glæden ved faget. Jytte Post Petersen er en af musikterapiens store...

  17. Post-Cruise Questionnaire - Legacy

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Post-Cruise Questionnaire is a mandatory post trip legal document that observers fill out after every trip they have completed.

  18. Retention of Root Canal Posts

    DEFF Research Database (Denmark)

    Sahafi, A; Benetti, Ana Raquel; Flury, S

    2015-01-01

    The aim of this study was to investigate the effect of the cement film thickness of a zinc phosphate or a resin cement on retention of untreated and pretreated root canal posts. Prefabricated zirconia posts (CosmoPost: 1.4 mm) and two types of luting cements (a zinc phosphate cement [DeTrey Zinc...... received tribochemical silicate coating according to the manufacturer's instructions. Posts were then luted in the prepared root canals (n=30 per group). Following water storage at 37°C for seven days, retention of the posts was determined by the pull-out method. Irrespective of the luting cement......, pretreatment with tribochemical silicate coating significantly increased retention of the posts. Increased cement film thickness resulted in decreased retention of untreated posts and of pretreated posts luted with zinc phosphate cement. Increased cement film thickness had no influence on retention...

  19. Post-traumatic syringomyelia

    International Nuclear Information System (INIS)

    Freund, M.; Sartor, K.; Aschoff, A.; Spahn, B.

    1999-01-01

    The improvement of preclinical emergency medicine, better surgical and conservative therapies, and the development of intensive care units and specialized centers have improved the survival rate for patients with serious spinal cord injuries. Therefore, more sequelae of chronic spinal cord injuries such as post-traumatic spinal cord cavitations also occur. The first such case was described by Bastian in 1867. Generally, these cavitations were diagnosed from 2 months up to 32 years after the trauma. The overall prevalence of post-traumatic syringomyelia (PTS) is not known; however, with the increasing use of magnetic resonance imaging (MRI), its diagnosis has increased, ranging from 2.3% of paraplegic and tetraplegic patients in 1976 and 3.2% in 1985, to nearly 50% in a selected group of patients in 1991 and 1993. In 1995, a 4.45% incidence was reported. In our clinic we are currently treating 440 cases of syringomyelia, 140 of which are PTS. Several observations suggest more than one potential mechanism for the evolution of a post-traumatic cyst or PTS. Various factors, such as hemorrhage or, in particular, ischemia within the spinal cord, blockage of the cerebrosinal fluid (CSF) pathways around the cord or localized meningeal fibrosis either alone or in combination with other factors, may be involved. Clinically, sensory disturbances, loss of motor function, pain, and modification of the deep tendon reflexes are observed in most patients. On MRI, PTS is seen as a longitudinal, cystic cavity within the spinal cord, giving a hypointense signal on T 1 -weighted images and a hyperintense signal on T 2 -weighted images. For treatment planning it is mandatory to identify the lower and upper end of the PTS on the MRI. (orig.) [de

  20. Post Rule of Law

    DEFF Research Database (Denmark)

    Carlson, Kerstin Bree

    2016-01-01

    The value of developing hybrid international criminal procedure (ICP) is that it is arguably inclusive (representing two major legal traditions) and distinct from any domestic system, thus creating a separate, sui generis realm for international criminal law (ICL) jurists to meet. Since its...... addresses the practice of hybridity in ICP, drawing examples from the construction and evolution of hybrid procedure at the International Criminal Tribunal for the Former Yugoslavia (ICTY), to argue that the hybridity practiced by international criminal tribunals renders them ‘post rule of law’ institutions...

  1. The post Chernobyl society

    International Nuclear Information System (INIS)

    Xenofontov, Ion.

    2011-01-01

    The disaster from the nuclear power plant in Chernobyl that took place on April 26, 1986 is considered to be the worst ecologic disaster in Europe during the entire nuclear power producing history (estimated on the highest level, the seventh). The disaster had an poisonous impact on people's health and ambitions, it also gave birth to a new vision on the impact of the human factor on the universe. The post Chernobyl society is an alarming sign as regarding the human surviving perspectives, and a violent lesson on the 'global biography'. (author)

  2. Post-tonsillectomy hemorrhage

    DEFF Research Database (Denmark)

    Heidemann, Christian; Wallén, Mia; Aakesson, Marie

    2008-01-01

    Post-tonsillectomy hemorrhage (PTH) is a relatively common and potentially life-threatening complication. The objective of this study was to examine the rate of PTH and identify risk factors. A retrospective cohort study was carried out including all tonsillectomies (430 patients) performed...... as surgical technique" [relative risk (RR) = 5.3], "peritonsillar abscess as indication for surgery" (RR = 0.3) and "age equal to or above 15 years at the time of surgery" (RR = 5.4). It is concluded that patient age, PTA as indication for surgery and the use of coblation significantly affect the occurrence...

  3. Post-Quantum Cryptography

    DEFF Research Database (Denmark)

    Gauthier Umana, Valérie

    . The public key cryptosystems that can resist these emerging attacks are called quantum resistant or post-quantum cryptosystems. There are mainly four classes of public-key cryptography that are believed to resist classical and quantum attacks: code-based cryptography, hash-based cryptography, lattice......-based cryptography and multivariate public-key cryptography. In this thesis, we focus on the rst two classes. In the rst part, we introduce coding theory and give an overview of code-based cryptography. The main contribution is an attack on two promising variants of McEliece's cryptosystem, based on quasi...

  4. Post LS1 schedule

    CERN Document Server

    Lamont, M

    2014-01-01

    The scheduling limits for a typical long year taking into account technical stops, machine development, spe- cial physics runs are presented. An attempt is then made to outline a ten year post LS1 schedule taking into account the disparate requirements outlined in the previous talks in this session. The demands on the planned long shutdowns and the impact of these demands on their proposed length will be discussed. The option of using ion running as a pre-shutdown cool-down period will be addressed.

  5. Off-the-Shelf Virus-Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation.

    Science.gov (United States)

    Tzannou, Ifigeneia; Papadopoulou, Anastasia; Naik, Swati; Leung, Kathryn; Martinez, Caridad A; Ramos, Carlos A; Carrum, George; Sasa, Ghadir; Lulla, Premal; Watanabe, Ayumi; Kuvalekar, Manik; Gee, Adrian P; Wu, Meng-Fen; Liu, Hao; Grilley, Bambi J; Krance, Robert A; Gottschalk, Stephen; Brenner, Malcolm K; Rooney, Cliona M; Heslop, Helen E; Leen, Ann M; Omer, Bilal

    2017-11-01

    Purpose Improvement of cure rates for patients treated with allogeneic hematopoietic stem-cell transplantation (HSCT) will require efforts to decrease treatment-related mortality from severe viral infections. Adoptively transferred virus-specific T cells (VSTs) generated from eligible, third-party donors could provide broad antiviral protection to recipients of HSCT as an immediately available off-the-shelf product. Patient and Methods We generated a bank of VSTs that recognized five common viral pathogens: Epstein-Barr virus (EBV), adenovirus (AdV), cytomegalovirus (CMV), BK virus (BKV), and human herpesvirus 6 (HHV-6). The VSTs were administered to 38 patients with 45 infections in a phase II clinical trial. Results A single infusion produced a cumulative complete or partial response rate of 92% (95% CI, 78.1% to 98.3%) overall and the following rates by virus: 100% for BKV (n = 16), 94% for CMV (n = 17), 71% for AdV (n = 7), 100% for EBV (n = 2), and 67% for HHV-6 (n = 3). Clinical benefit was achieved in 31 patients treated for one infection and in seven patients treated for multiple coincident infections. Thirteen of 14 patients treated for BKV-associated hemorrhagic cystitis experienced complete resolution of gross hematuria by week 6. Infusions were safe, and only two occurrences of de novo graft-versus host disease (grade 1) were observed. VST tracking by epitope profiling revealed persistence of functional VSTs of third-party origin for up to 12 weeks. Conclusion The use of banked VSTs is a feasible, safe, and effective approach to treat severe and drug-refractory infections after HSCT, including infections from two viruses (BKV and HHV-6) that had never been targeted previously with an off-the-shelf product. Furthermore, the multispecificity of the VSTs ensures extensive antiviral coverage, which facilitates the treatment of patients with multiple infections.

  6. Shared consultant physician posts.

    LENUS (Irish Health Repository)

    Cooke, J

    2012-01-31

    Our aim was to assess the acceptability and cost-efficiency of shared consultancy posts. Two consultant physicians worked alternate fortnights for a period of twelve months. Questionnaires were distributed to general practitioners, nurses, consultants and junior doctors affected by the arrangement. Patients or their next of kin were contacted by telephone. 1\\/17 of consultants described the experience as negative. 14\\/19 junior doctors reported a positive experience. 11 felt that training had been improved while 2 felt that it had been adversely affected. 17\\/17 GPs were satisfied with the arrangement. 1\\/86 nurses surveyed reported a negative experience. 1\\/48 patients were unhappy with the arrangement. An extra 2.2 (p<0.001) patients were seen per clinic. Length of stay was shortened by 2.49 days (p<0.001). A saving of 69,212 was made due to decreased locum requirements. We present data suggesting structured shared consultancy posts can be broadly acceptable and cost efficient in Ireland.

  7. [Post-traumatic tics].

    Science.gov (United States)

    Alegre, S; Chacón, J; Redondo, L; Navarro-Busto, C; Solana, B

    1996-10-01

    Secondary tics are those in which an aetiology justifying them can be found, as compared to idiopathic tics, which make up the majority, and the Gilles de la Tourette syndrome (SGT), which is, at the moment, of unknown origin. Of the possible aetiologies described as causing tics, craneo-encephalic trauma has been mentioned on very few occasions. We present a case of post-traumatic tics (verbal and neck) in a young man of 24, and review the published cases which can be considered to be of post-traumatic tics. We have found six cases of tics secondary to traumas, all craneo-encephalic, like ours (the one under study). The time interval between the blow and the appearance of the tic or tics varied between 2 weeks and 3 months. The absence of significant lesions seen in the complementary investigations make it impossible for us to discover the site of the lesion caused by the trauma. However, the presence in some cases of other tics before the trauma, and of family histories of tics, supports the idea of a genetic basis or predisposition to suffer this disorder.

  8. Independents' group posts loss

    International Nuclear Information System (INIS)

    Sanders, V.; Price, R.B.

    1992-01-01

    Low oil gas prices and special charges caused the group of 50 U.S. independent producers Oil and Gas Journal tracks to post a combined loss in first half 1992. The group logged a net loss of $53 million in the first half compared with net earnings of $354 million in first half 1991, when higher oil prices during the Persian Gulf crisis buoyed earnings in spite of crude oil and natural gas production declines. The combined loss in the first half follows a 45% drop in the group's earnings in 1991 and compares with the OGJ group of integrated oil companies whose first half 1992 income fell 47% from the prior year. Special charges, generally related to asset writedowns, accounted for most of the almost $560 million in losses posted by about the third of the group. Nerco Oil and Gas Inc., Vancouver, Wash., alone accounted for almost half that total with charges related to an asset writedown of $238 million in the first quarter. Despite the poor first half performance, the outlook is bright for sharply improved group earnings in the second half, assuming reasonably healthy oil and gas prices and increased production resulting from acquisitions and in response to those prices

  9. Medicamentos utilizados em transplante de medula óssea: um estudo sobre combinações dos antimicrobianos potencialmente interativos Medicamentos utilizados en casos de trasplante de médula ósea: un estudio sobre combinaciones antimicrobianas potencialmente interactivas Drugs used in bone marrow transplantation: a study about combinations of antimicrobial potentially interactives

    Directory of Open Access Journals (Sweden)

    Rosimeire Barbosa Fonseca

    2008-12-01

    Full Text Available O objetivo do estudo foi caracterizar o perfil dos medicamentos e identificar combinações decorrentes da co-administração de antimicrobianos potencialmente interativos e outros agentes. 70 prescrições médicas de pacientes submetidos a transplante de medula óssea (TMO, todos internados no Instituto do Coração, São Paulo, Brasil, foram analisadas. Os medicamentos foram classificados de acordo com o sistema Alfa, e o potencial de interação e as características farmacológicas foram listados a partir da literatura. Na analise dos dados utilizou-se estatística descritiva. Verificou-se que 72,7% dos medicamentos apresentaram potencial interativo, destacando-se os precipitadores (79,2% e o fluconazol (85,7% como o antimicrobiano mais envolvido nas combinações, associado ao omeprazol em 40% da amostra. Nos pacientes de TMO, a co-administração de medicamentos potencialmente interativos foi freqüente, condição que, associada à polifarmácia e ao aprazamento simultâneo de horários na administração desses agentes, poderia predispor o paciente a eventos indesejados, afetando, deste modo, a segurança da terapia.El objetivo del estudio fue determinar el perfil de medicamentos e identificar combinaciones por administración conjunta de antimicrobianos potencialmente interactivos y otros agentes. Fueron analizadas 70 prescripciones médicas de pacientes sometidos a trasplante de médula ósea (TMO, todos internados en el Instituto del Corazón, São Paulo, Brasil. Los medicamentos fueron clasificados según el sistema Alfa. El potencial de interacción y las características farmacológicas fueron establecidas según la bibliografía. El análisis de datos a través de estadística descriptiva. El 72,7% de los medicamentos mostró potencial interactivo, destacándose los precipitadores (79,2% y como antimicrobiano más utilizado en las combinaciones el fluconazol (85,7%, siendo asociado al omeprazol en 40% de la muestra. La combinaci

  10. Trasplante de células progenitoras derivadas de la médula ósea y factor de crecimiento granulocítico en cardiopatía isquémica aguda y crónica Transplant of stem cells derived from bone marrow and granulocytic growth factor in acute and chronic ischemic myocardiopathy

    Directory of Open Access Journals (Sweden)

    Juan M Senior

    2007-12-01

    Full Text Available Introducción: estudios recientes demuestran la seguridad y eficacia de la implantación de células progenitoras derivadas de la médula ósea y de la administración del factor estimulante de colonias de granulocito en pacientes con infarto agudo del miocardio con elevación del segmento ST y en cardiopatía isquémica crónica. Se diseñó un estudio prospectivo, abierto de «antes y después» para evaluar la seguridad y eficacia de la terapia celular asociada a la administración del factor de crecimiento. Se reporta la primera experiencia con este tipo de terapia. Metodología: este es el reporte del seguimiento a seis meses, de los pacientes con cardiopatía isquémica aguda y crónica a quienes se les realizó trasplante de células progenitoras derivadas de la médula ósea, movilizadas con factor de crecimiento estimulante de colonias de granulocitos, por vía intracoronaria o epicárdica. Se incluyeron dos grupos de pacientes: 1. Diez pacientes con infarto de pared anterior y 2. Cinco pacientes con cardiopatía isquémica crónica, todos con necrosis extensa demostrada por ausencia de viabilidad miocárdica por medicina nuclear y fracción de eyección menor del 40%. Resultados: se demostró mejoría significativa de la fracción de eyección de 29,44 ± 3,36 a 37,6 ± 5,3 con pIntroduction: recent studies have shown the safety and efficacy of the stem cells derived from bone marrow (BMC implant with concomitant administration of stimulating factor of granulocyte colonies in patients with acute myocardial infarction with ST segment elevation and in chronic ischemic cardiopathy. An open prospective «before and after» design was made to evaluate the safety and efficacy of cell therapy associated to growth factor administration. The first experience with this kind of therapy is reported. Methodology; this is a 6 months follow-up report of patients with acute and chronic ischemic cardiopathy to whom transplant of stem cells derived from

  11. Drug interactions of anti-microbial agents used in hematopoietic stem cell transplantation Interacciones medicamentosas de antimicrobianos utilizados en trasplante de células madre hematopoyéticas Interações medicamentosas de antimicrobianos utilizados em transplante de células-tronco hematopoéticas

    Directory of Open Access Journals (Sweden)

    Rosimeire Barbosa Fonseca Guastaldi

    2011-08-01

    Full Text Available This study analyzed potential drug interactions (PDIs of antimicrobials used in patients of hematopoietic stem cell transplantation and identified associated factors. The sample consisted of 70 patients admitted to a hospital in São Paulo. The PDIs were analyzed through the consultation of the Drug Interactions Facts and Drug Interactions Handbook. Descriptive statistics and logistic regression were used. Half of the sample was exposed to 13 PDIs, which occurred with fluconazole (53.8%, ciprofloxacin (30.8% and sulfamethoxazole-trimethoprim (15.4%. Most (92.3% were of moderate severity, with good evidence (61.6%, early delayed effect (61.5% and need to have their therapy monitored (76.9%. Patients with four or more medications (pEl estudio analizó interacciones medicamentosas potenciales (IMP de antimicrobianos usados en pacientes sometidos a trasplante de células madre hematopoyéticas e identificó los factores asociados las IMP. La casuística fue compuesta por 70 pacientes internados en un Hospital de Sao Paulo. Las IMP fueron a través de la consulta al Drug Interactions Facts y Drug Interactions Handbook. En el análisis de los datos se utilizó estadística descriptiva y regresión logística. Mitad de la muestra fue expuesta a 13 IMP, que ocurrieron con fluconazol (53,8%, ciprofloxacina (30,8% y sulfametoxazol+trimetoprima (15,4%. La mayoría (92,3% presentó gravedad moderada, inicio de efecto demorado (61,5% y necesidad de monitorizar la terapia (76,9%. Cuatro o más medicamentos (pNeste estudo, analisaram-se as interações medicamentosas potenciais (IMP de antimicrobianos, usados em pacientes submetidos a transplante de células-tronco hematopoiéticas e foram identificdos os fatores associados às IMPs. A casuística foi composta por 70 pacientes internados em hospital do município de São Paulo. As IMPs foram analisadas através da consulta ao Drug Interactions Facts e Drug Interactions Handbook. Na análise dos dados, utilizou

  12. Trasplante autólogo de células progenitoras derivadas de la médula ósea, por vía intramiocárdica, para revascularización en cardiopatía isquémica crónica Autologous intramyocardial transplant of bone marrow derived stem cells for revascularization in ischemic chronic cardiopathy

    Directory of Open Access Journals (Sweden)

    Óscar Velásquez

    2005-08-01

    Full Text Available Introducción: estudios experimentales y clínicos demuestran que la implantación intramiocárdica de células progenitoras derivadas de la médula ósea, produce angiogénesis y mejora la función cardiaca en pacientes con cardiopatía isquémica crónica. Metodología: este es el reporte del seguimiento a dos meses de un paciente con cardiopatía isquémica crónica no susceptible de revascularización quirúrgica o percutánea, a quien se le realizó ,por vía epicárdica, un implánte de células progenitoras derivadas de médula ósea movilizadas con factor de crecimiento granulocito-macrófago. Resultados: se demostró mejoría significativa en la sintomatología, en la capacidad funcional (1.8 MET a 10 MET, en el estado funcional (IV a I, en el tamaño del defecto de perfusión miocárdica por medicina nuclear, en la fracción de eyección y en la disminución del volumen sistólico final del ventrículo izquierdo, sin observar complicaciones relacionadas con el procedimiento. Conclusión: el trasplante de células progenitoras derivadas de la médula ósea es una terapia efectiva y segura para promover neovascularización y para mejorar la contractilidad y la perfusión miocárdica en los pacientes con cardiopatía isquémica crónica pobres candidatos a cirugía.Introduction: experimental and clinical studies have demonstrated that intramyocardial bone marrow derived stem cells implantation produce angiogenesis and improvement of cardiac function in patients with chronic ischemic cardiopathy. Methodology: this is a two months follow-up report of a patient with chronic ischemic cardiopathy not susceptible of surgical or percutaneous revascularization, to whom bone marrow stem cells mobilized with granulocyte - macrophage growth factor was implanted. Results: significant improvement was demonstrated in symptomatology, functional capacity (1.8 Mets to 10 Mets, functional state (IV to I, size of the myocardial perfusion defect by nuclear

  13. Post-traumatic hemobilia

    International Nuclear Information System (INIS)

    Vlahos, L.; Kalovidouris, A.; Gouliamos, A.; Kailidou, E.

    1991-01-01

    Four patients with post-traumatic hemobilia were evaluated with arteriography over a 2-year period. In two patients hemobilia was of iatrogenic origin; in particular, one case appeared after a cholecystectomy, and the other was due to placement of a biliary stent with an endoscope. In the other two patients hemobilia was the result of a gun injury. Arteriography of the hepatic arterial system demonstrated two false aneurysms, extravasation of contrast medium through the biliary system in one patient and arterioportal fistula in another patient. It is concluded that arteriography of the hepatic arterial system is the method of choice for the evaluation and the possible treatment of patients with hemobilia. (author). 20 refs.; 4 figs

  14. Post-traumatic hemobilia

    Energy Technology Data Exchange (ETDEWEB)

    Vlahos, L.; Kalovidouris, A.; Gouliamos, A.; Kailidou, E. (Athens Univ. (Greece))

    Four patients with post-traumatic hemobilia were evaluated with arteriography over a 2-year period. In two patients hemobilia was of iatrogenic origin; in particular, one case appeared after a cholecystectomy, and the other was due to placement of a biliary stent with an endoscope. In the other two patients hemobilia was the result of a gun injury. Arteriography of the hepatic arterial system demonstrated two false aneurysms, extravasation of contrast medium through the biliary system in one patient and arterioportal fistula in another patient. It is concluded that arteriography of the hepatic arterial system is the method of choice for the evaluation and the possible treatment of patients with hemobilia. (author). 20 refs.; 4 figs.

  15. Post dengue neurological complication

    Directory of Open Access Journals (Sweden)

    Hizlinda Tohid

    2015-12-01

    Full Text Available Dengue infection is highly endemic in many tropical countries including Malaysia. However, neurological complications arising from dengue infection is not common; Gullain–Barre syndrome (GBS is one of these infrequent complications. In this paper, we have reported a case in which a 39-year-old woman presented with a neurological complication of dengue infection without typical symptoms and signs of dengue fever. She had a history of acute gastroenteritis (AGE followed by an upper respiratory tract infection (URTI weeks prior to her presentation rendering GBS secondary to the post viral URTI and AGE as the most likely diagnosis. Presence of thrombocytopenia was the only clue for dengue in this case.

  16. [Enterostomy post emergency enterectomy].

    Science.gov (United States)

    Gavrilescu, S; Velicu, D; Gheorghiu, L; Duţescu, S

    2002-01-01

    This is a clinical approach regarding 43 resection of intestine, performed in emergency condition, terminated as enterostomy, with represent 20% of enterectomyes performed in emergency condition and 1.6% of urgent operations. The decision of enterostomy has been taken in the conditions of peritoneal sepsis, occlusion or the association of the two circumstances. The results are comparatively analyzed between the cases with enterostomy that has been made from the beginning (66% success, 33% gone wrong), and those with enterostomy made at the second intervention (14% success, 86% gone wrong). One discusses problems of leading, technique and post-operating nursing. The intestinal reintegration has been made possible at 16 patients after a timing of three of four weeks.

  17. Post-streptococcal glomerulonephritis

    Directory of Open Access Journals (Sweden)

    Odalovic A.

    2014-01-01

    Full Text Available Post-streptococcal glomerulonephritis (PSGN is a frequent cause of acute nephritis in children. This case study was done with the aim to point out that the infections caused by Group A streptococci, in spite of antibiotic era, are still present in the population. An 8-year old boy was admitted in our hospital with a two-day history of fewer, tonsillopharyngitis. After hospital admission, patient was treated with penicillin during the period of 10 days, antihypertensive medications (captopril, furosemide, including restricted diet of salt. After the treatment, patient became better. On demission it was found proteinuria and microhematuria PSGN is very serious disease, which leaves severe complications if the valid therapy with penicillin is not used in propriety time, during the recommended period of 10 days.

  18. Post-representational cartography

    Directory of Open Access Journals (Sweden)

    Rob Kitchin

    2010-03-01

    Full Text Available Over the past decade there has been a move amongst critical cartographers to rethink maps from a post-representational perspective – that is, a vantage point that does not privilege representational modes of thinking (wherein maps are assumed to be mirrors of the world and automatically presumes the ontological security of a map as a map, but rather rethinks and destabilises such notions. This new theorisation extends beyond the earlier critiques of Brian Harley (1989 that argued maps were social constructions. For Harley a map still conveyed the truth of a landscape, albeit its message was bound within the ideological frame of its creator. He thus advocated a strategy of identifying the politics of representation within maps in order to circumnavigate them (to reveal the truth lurking underneath, with the ontology of cartographic practice remaining unquestioned.

  19. Post-traumatic pseudomyopia.

    Science.gov (United States)

    London, Richard; Wick, Bruce; Kirschen, David

    2003-02-01

    Many clinicians have noted that patients demonstrate a myopic refractive change following Traumatic Brain Injury (TBI). This apparent myopic shift disappears with cycloplegia, yet stubbornly reappears as soon as the pharmaceutical effect wears off. We propose that this shift is secondary to an irritative lesion that affects the parasympathetic innervation, resulting in ciliary body contracture. The dilemma for the clinician is whether to provide the immediate relief of clear distance vision by prescribing additional minus lenses, or to work toward attempting to re-establish the baseline refractive error. The natural history of post-traumatic pseudomyopia in our experience involves one of the following three courses: (1) a transient condition that will occasionally resolve; (2) the typical case, a recalcitrant condition that will resolve under cycloplegic intervention, but immediately return as the cycloplegic wears off; or (3) a less-common subgroup of patients who continue to show an increase in myopia over time. Our description of these cases demonstrates management strategies (including atropinization) to relax accommodative spasm, traditional vision therapy techniques aimed at loosening the accommodative system, and refractive corrections. Pseudomyopia is one of many ocular and behavioral sequelae following TBI. By understanding the natural course and potential management options for post-traumatic pseudomyopia, the clinician will be better prepared to deal with these challenging cases. Flexibility is required, since options that work with one patient may prove ineffective with another. Counseling the patient as to potential outcomes given the natural history of this condition helps establish more-realistic expectations by the patients being treated.

  20. Not being able to live like before: the family dynamics during the experience of pediatric liver transplantation No pudiendo vivir como antes: la dinámica familiar durante la experiencia de trasplante de hígado en niños Não podendo viver como antes: a dinâmica familiar na experiência do transplante hepático da criança

    Directory of Open Access Journals (Sweden)

    Ana Márcia Chiaradia Mendes

    2009-02-01

    Full Text Available This study aims to understand families' dynamics during the experience of pediatric liver transplantation, and to identify families' demands and resources. Symbolic interactionism was used as the theoretical framework and grounded theory as the methodological reference. Data were collected through semi-structured interviews with eight families at a public hospital in Sao Paulo, SP, Brazil. Two phenomena were identified: having life controlled by the transplantation represents the vulnerability of families experiencing uncertainty and fear during their children's disease experience; and struggling to reacquire autonomy refers to families' reaction when exposed to the first phenomenon, which consists of continuous adaptation to overcome suffering caused by the situation. The relationship of these two phenomena allowed for the identification of the central category: not being able to live like before. Based on this analysis, a theoretical model could be proposed to explain the experience.Esta investigación tuvo como objetivos entender la dinámica familiar durante la experiencia del trasplante de hígado pediátrico e identificar las demandas y los recursos de la familia. El estudio utilizó como marco teórico el Interaccionismo Simbólico y como marco metodológico la Teoría Fundamentada en los Datos. Los datos fueron recolectados durante entrevistas semiestructuradas con 8 familias en un hospital escuela en el municipio de San Pablo. Fue posible identificar dos fenómenos que componen esta experiencia: teniendo la vida controlada por el trasplante, que representa la vulnerabilidad de la familia al vivir las incertidumbres y el miedo constante durante la experiencia de la enfermedad del niño; y, luchando para rescatar la autonomía, que consiste en el movimiento de reacción de la familia para enfrentar el primer fenómeno, adaptándose continuamente para enfrentar el estrés y el sufrimiento desencadenados por la situación. La articulaci

  1. Thrombotic obstruction of the central venous catheter in patients undergoing hematopoietic stem cell transplantation Obstrucción trombótica del catéter venoso central en pacientes sometidos al trasplante de células-tronco hematopoyéticas Obstrução trombótica do cateter venoso central em pacientes submetidos ao transplante de células-tronco hematopoéticas

    Directory of Open Access Journals (Sweden)

    Kátia Michelli Bertoldi Arone

    2012-08-01

    Full Text Available This is an integrative literature review with the aim of summarizing the prevention measures and treatment of thrombotic obstruction of long-term semi-implanted central venous catheters, in patients undergoing hematopoietic stem cell transplantation. The sample consisted of seven studies, being two randomized controlled clinical trials, three cohort studies and two case series. Regarding the prevention measures, one single study demonstrated effectiveness, which was a cohort study on the oral use of warfarin. In relation to the treatment measures, three studies evidenced effectiveness, one highlighted the efficacy of streptokinase or urokinase, one demonstrated the benefit of using low-molecular-weight heparin and the other treated the obstruction with heparin or urokinase. Catheter patency research shows a restricted evolution that does not follow the evolution of transplantations, mainly regarding nursing care.Se trata de una revisión integradora de la literatura con objeto de sintetizar las medidas de prevención y tratamiento de obstrucción trombótica del catéter venosos central de larga permanencia y semi-implantado, en pacientes sometidos al trasplante de células-tronco hematopoyéticas. La muestra abarcó a siete estudios: dos ensayos clínicos controlados aleatorizados, tres estudios de cohorte y dos series de casos. Respecto a las medidas de prevención, fue identificado un único estudio efectivo, uno cohorte sobre el uso de la warfarina oral. Sobre las medidas de tratamiento, tres estudios evidenciaron efectividad, uno apuntó la eficacia de la estreptoquinasa o uroquinasa, otro mostró beneficio del uso de heparina de bajo peso molecular y otro trató la obstrucción con heparina o uroquinasa. Se observa que la evolución de la investigación sobre la permeabilidad del catéter fue limitada, no acompañando la evolución del trasplante, principalmente respecto a los cuidados de enfermería.Trata-se de revisão integrativa da

  2. Post retention and post/core shear bond strength of four post systems.

    Science.gov (United States)

    Stockton, L W; Williams, P T; Clarke, C T

    2000-01-01

    As clinicians we continue to search for a post system which will give us maximum retention while maximizing resistance to root fracture. The introduction of several new post systems, with claims of high retentive and resistance to root fracture values, require that independent studies be performed to evaluate these claims. This study tested the tensile and shear dislodgment forces of four post designs that were luted into roots 10 mm apical of the CEJ. The Para Post Plus (P1) is a parallel-sided, passive design; the Para Post XT (P2) is a combination active/passive design; the Flexi-Post (F1) and the Flexi-Flange (F2) are active post designs. All systems tested were stainless steel. This study compared the test results of the four post designs for tensile and shear dislodgment. All mounted samples were loaded in tension until failure occurred. The tensile load was applied parallel to the long axis of the root, while the shear load was applied at 450 to the long axis of the root. The Flexi-Post (F1) was significantly different from the other three in the tensile test, however, the Para Post XT (P2) was significantly different to the other three in the shear test and had a better probability for survival in the Kaplan-Meier survival function test. Based on the results of this study, our recommendation is for the Para Post XT (P2).

  3. Post-16 update

    Science.gov (United States)

    1999-01-01

    Post-16 Initiative logo This is the first of a regular series of contributions from the Institute's Post-16 Initiative. The Initiative is taking a hard and searching look at the physics taught in schools and colleges from age 16 to age 19. To start with, it is responding to Government initiatives, but hopes to encourage and stimulate good practice in physics teaching on a longer time scale than can be afforded in making responses to current developments. Here Jon Ogborn writes about what AS courses need to be, while Peter Campbell gives his thoughts about teaching matter. Advanced Subsidiary physics: what should it be? From September 2000 all A-levels will be new. Students can take the first Advanced Subsidiary (AS) year and stop there - or decide to go on. In the Institute of Physics post-16 Initiative, we have been thinking how to provide a satisfying one-year experience of physics at the new AS level, and what it should achieve. The students will decide. So the AS course must give a decent picture of what physics is, what it offers for their futures, what interests it can satisfy. That all says breadth, with enough depth to see what is in store later. And this sounds like the right recipe for someone who is taking a single AS year of physics to broaden their A-level experience. It must also be attractive. A way forward is shown by the Salters - Horners course, attracting interest through leading from applications. Why does that work? It gives physics a story to tell, into which ideas fit and make sense. Our own new A-level, Advancing Physics, must also have interesting stories to tell, which must in addition build up an honest picture of physics. An example: teach electric circuits through modern sensing devices. Sensor instrumentation is a key activity of physicists, full of new ideas, but also simple. It makes essential use of circuits such as the potential divider. Practical work gets better things to do than checking the equation for resistors in parallel

  4. Post-16 update

    Science.gov (United States)

    1999-07-01

    (post16) Faces of Physics To study a physics course post-16 is take out an option on your future. But physics itself is very varied, and so is what you might do with it. It seems right that post-16 physics courses reflect important aspects of this variety. Physics varies with why people do it. As reliable knowledge, physics is often essential to making things happen. This is physics as feeding into technology, and quite often feeding off technology. The human urge here is the urge to create things that work. It drives individual inventors; it drives large teams in multinational companies. At the opposite end of this spectrum is physics driven by curiosity, by the urge to find out. The stock examples are the grand discoveries from the expansion of the universe to the discovery of the nucleus of atoms. But the physicist's curiosity is often on a more detailed, even finicky scale: how exactly do the molecules of this polymer respond to stress?; just how do ions implanted in this material modify its conductivity? It is clear to me that a decent post-16 physics course must respect and reflect both. This is by no means a matter of `basic laws and their applications'. It is a matter of recognizing two fundamental interests, in doing and in explaining, and of recognizing that they are at once closely interdependent and worlds apart. Ideas in physics are also not of one kind. One opposition is the idea of describing Nature in terms of deterministic physical laws, as against describing Nature as the predictable outcome of probabilistic behaviour `underneath'. The first has gripped the Western imagination since at least the time of Descartes. The second has increasingly come to the fore, especially in thermodynamics with the idea that we can only make processes work by fixing the circumstances so that uncaring atoms and molecules happen by chance to do what we want. Now in quantum theory both ideas - determinism and randomness - co-exist. Post-16 physics courses are not in

  5. IVGEN Post Flight Analysis

    Science.gov (United States)

    Mcquillen, John; Brown, Dan; Hussey, Sam; Zoldak, John

    2014-01-01

    The Intravenous Fluid Generation (IVGEN) Experiment was a technology demonstration experiment that purified ISS potable water, mixed it with salt, and transferred it through a sterilizing filter. On-orbit performance was verified as appropriate and two 1.5 l bags of normal saline solution were returned to earth for post-flight testing by a FDA certified laboratory for compliance with United States Pharmacopiea (USP) standards. Salt concentration deviated from required values and an analysis identified probable causes. Current efforts are focused on Total Organic Content (TOC) testing, and shelf life.The Intravenous Fluid Generation (IVGEN) Experiment demonstrated the purification of ISS potable water, the mixing of the purified water with sodium chloride, and sterilization of the solution via membrane filtration. On-orbit performance was monitored where feasible and two 1.5-liter bags of normal saline solution were returned to earth for post-flight testing by a FDA-registered laboratory for compliance with United States Pharmacopeia (USP)standards [1]. Current efforts have been focused on challenge testing with identified [2] impurities (total organic-carbon), and shelf life testing. The challenge testing flowed known concentrations of contaminants through the IVGEN deionizing cartridge and membrane filters to test their effectiveness. One finding was that the filters and DI-resin themselves contribute to the contaminant load during initial startup, suggesting that the first 100 ml of fluid be discarded. Shelf life testing is ongoing and involves periodic testing of stored DI cartridges and membrane filters that are capped and sealed in hermetic packages. The testing is conducted at six month intervals measuring conductivity and endotoxins in the effluent. Currently, the packaging technique has been successfully demonstrated for one year of storage testing. The USP standards specifies that the TOC be conducted at point of generation as opposed to point of

  6. Post commissioning noise study

    International Nuclear Information System (INIS)

    Heraud, P.

    2008-01-01

    This presentation described a wind farm post-commissioning study conducted at a wind farm owned by Helimax Energy Inc. The farm was located in a partly-forested, partly cultivated region in Quebec that featured gently rolling hills. Over 600 dwellings were located within 2 km of the wind farm, and 44 dwellings were within the wind farm's boundaries. The noise impact assessments were conducted at various points near the wind farm. The wind farm was designed using an International Standards Organization (ISO) noise propagation model and a 40 dBA to provide adequate setbacks. The study was conducted using 10 days of continuous measurements at selected points of a wind farm. Points of reception included points from 650 m to 800 m. Noise over 2 km was not thought to be contributed by the wind turbine. The nearest dwelling was 512 m from one of the farm's wind turbines. The study also considered ground factor, temperature, relative humidity, and the height of the receptors. Quebec noise level limits are 40 dBA at night, and 45 dBA during the day. Noise level limits are independent of wind speed. Measured noise contributions over 40 dBA were not observed during the measurement program. The wind turbines were only audible for 1 night out of the 30 night study period. It was concluded that the ISO noise propagation model is a reliable tool for conducting noise impact assessments. tabs., figs

  7. NEWS: Post-16 update

    Science.gov (United States)

    Campbell, Peter

    2000-07-01

    post16.gif As a teacher of physics it is very easy to become preoccupied with particulars of courses, or topics or even single concepts. Concerned with imminent student audiences and desired learning outcomes, the daily challenge is to summon satisfactory teaching approaches and resources for the job at hand. For the conscientious teacher, assessment outcomes may too often seem a judgment on our own efforts rather than those of our students. From time to time we may step back and think bigger, for example while planning a recruitment event, or while away from work on holiday. We may be successful locally. But why, at a time when books and television documentaries popularizing science have a large following, has physics education been facing declining numbers? Many recognize that physics has an essential contribution to make to the training of science or engineering specialists, but we know that it is also important for the skilled worker, the informed citizen and, in fact, for anyone trying to make sense of the world. So what are the best ways forward for post-16 physics? To make any impact on the bigger picture requires organization, thinking and meeting time among people in diverse roles: teachers and curriculum managers; university lecturers; employers and professional bodies; unitary awarding bodies; regulatory and funding agencies; and even Government. For the past few years, the Institute of Physics post-16 Initiative has created an unrivalled opportunity to address the wider issues. Its Shaping the Future booklets series was designed to stimulate informed discussion and debate, by providing background information and analysis. Taken together, the booklets should help all those concerned with physics education to understand where we are now, and why. Literally dozens of people have contributed to a review and analysis of physics education. Each booklet is a 48-page smorgasbord in A4 landscape format, containing many examples of good practice, basic but

  8. Post-ovulatory contraception.

    Science.gov (United States)

    Glasier, A; Baird, D T

    1990-06-01

    It is possible to prevent pregnancy after unprotected intercourse by suppressing ovulation, inhibiting fertilization or interfering with tubal transport and/or implantation of the early embryo. IUCDs probably prevent implantation by stimulating the release of prostaglandins from the endometrium but are not acceptable to many women. Post-coital contraceptive steroids, e.g. high-dose oestrogens, are associated with a relatively high incidence of side-effects and must be taken within 72 hours of coitus. As these agents are effective by creating a uterine environment unfavourable for implantation, it may be possible to use antigestagens or antioestrogens in this way. It is already known that an antigestagen in combination with a prostaglandin is a highly effective method of inducing abortion in very early pregnancy. The corpus luteum is essential for the maintenance of pregnancy and its destruction by a luteolytic agent should dislodge the implanting embryo. If an effective method of preventing implantation could be developed which was relatively free from side-effects, it should be possible to use it as a regular form of contraception to be taken only when the risk of pregnancy had occurred.

  9. Battery Post-Test Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Post-test diagnostics of aged batteries can provide additional information regarding the cause of performance degradation, which, previously, could be only inferred...

  10. NEWS: Post-16 update

    Science.gov (United States)

    1999-05-01

    (post16) Making physics connect Doesn't Melvyn Bragg do a wonderful job, engaging both scientists and artists in sensitive discussion on Radio 4 about their methods and their purposes? But every week teachers have the chance to show their students that physics is a way of seeing the world that is well-connected with other aspects of knowledge and culture. The stakes are high: students who fail to appreciate this generally choose not to study the subject beyond GCSE. Most students find our preoccupation with technical detail off-putting. Accepting that we have a syllabus to cover, it's still a question of balance. In our teaching we should aim for variety in order to find ways to connect with every student's interests. Also, we can show that we (the nearest embodiment of a physicist some students will experience) are multidimensional and so fully human. Most important, teachers need flexibility to both encourage and respond to student comment and questions. The first booklet in the discussion series Shaping the Future takes up these themes. Rich in ideas for both immediate use and the longer term, it aims to stimulate debate and improve teaching. Copies cost £5.50 including postage and are available from Ingrid Ebeyer, Post-16 Initiative, Institute of Physics, 76 Portland Place, London W1N 3DH. How far is it? This question is asked in many family cars and school minibuses at the start of a journey, and answered by most in terms of hours and minutes rather than miles. What a good idea for introducing a social and historical perspective to a lesson on distance, velocity and time. How far can you actually get in a day? What is the range of human activity? Walking for eight hours will get many people about 25 miles. A pack horse will progress at much the same rate, but fast riding or a coach and team of horses will get further. Motorway driving (when the cones are on holiday) would take you nearly 500 miles. The 05.15 am train from Penzance arrives in Inverness at 7

  11. Post-16 update

    Science.gov (United States)

    1999-11-01

    (Post-16 Initiative) Engineering Physics? Many A-level physics students do not go on to study physics. For them physics is a support subject, either just for fun or just for the grade. Where physics is a lead subject some students go on to study physics but many more go on to study engineering. So can we deliberately give some aspects of an A-level course an engineering flavour? Electromagnetism would seem a good place to start. There is a clear `physics' route into this topic, a microscopic forces and fields view of the situation. But do our students really need to look at it this way? All electromagnetic machines are linked magnetic and electric circuits. The design idea is to link these circuits as closely as possible. The electric circuits must be as good as possible, with a high conductivity. The magnetic circuits must be as good as possible, with high permeance. Conductivity depends on area/length. So does permeance. The goodness of an electromagnetic machine (how good it is at its job, which is linking electric and magnetic circuits) scales as the square of its linear dimensions. That means small electromagnetic machines are harder to make, and so the very smallest nanomotors are electrostatic. None of this is new, but many teachers are uncomfortable with it. We are thinking like physicists. Many of our students are not. They deserve us to take the trouble every now and again to encourage them to think a bit differently about a topic, to look at practical ways of discussing design and to give their course an engineering flavour. Philip Britton Coursework in A-level Physics The criteria for the new AS and A-levels have provided the teams developing the specifications with an opportunity to think creatively about how internal assessment is used within post-16 physics courses. Teachers may be concerned that allowing 30% of the marks to be internally assessed will create a burden for them. However, it is possible to look at this in a much more positive light

  12. Post-Baccalaureate Fellowship Program.

    Science.gov (United States)

    Cadbury, Jr., William E.

    The Post-Baccalaureate Fellowship Program provides an opportunity for students who went to college with inferior preparation to supplement their education by studying for 1 year at an academically demanding liberal arts college before entering graduate or professional school. The post-baccalaureate fellows take regular courses in a program that is…

  13. BK channel activators and their therapeutic perspectives

    DEFF Research Database (Denmark)

    Bentzen, Bo Hjorth; Olesen, Søren-Peter; Rønn, Lars C B

    2014-01-01

    in intracellular calcium to outward hyperpolarizing potassium currents. Consequently, the channel has many important physiological roles including regulation of smooth muscle tone, neurotransmitter release and neuronal excitability. Additionally, cardioprotective roles have been revealed in recent years. After...

  14. Post-16 update

    Science.gov (United States)

    1999-03-01

    Post-16 Initiative logo Physics in Mathematical Mood Later this year, as part of the post-16 initiative of the Institute of Physics, a booklet with the above title will be published. In draft form, the booklet was discussed at the ASE conference in January. Some of the issues raised are briefly set out here. If you have any views to contribute, please write to Simon Carson at the Institute of Physics or e-mail simon.carson@physics.org. A mathematical view of the world is intrinsically a part of physics and therefore physics should be studied in an appropriately mathematical way. However, we all know that, to some students at least, mathematics proves to be a stumbling block rather than a powerful aid to understanding. So how can we help? Realizing that as physics teachers we need to deliver the mathematics necessary to an understanding of our subject is a start. Its corollary is that we need to find the space within the physics core. We may wish to use supplementary courses such as AS mathematics or QCA's new free-standing mathematics units, but requiring additional courses as a prerequisite to a study of A-level physics may deter students. Teaching the mathematics in context may aid understanding but we also must ensure that techniques are seen in a variety of contexts and that at some point the tool is abstracted from the background. As teachers we need to be aware of the very basic mathematical difficulties that students bring with them: the use of calculators, standard form, simple algebraic manipulation, for example. Mathematical arguments need to be developed fully and carefully. Encouraging cooperation and discussion between students may help the less able to understand and the more able to appreciate and develop their own understanding through making explicit their reasoning by explaining it to others. And what of new technology? Software tools allow students to develop their understanding about graphs, for example, enabling them to investigate the

  15. Modelling redshift space distortion in the post-reionization H I 21-cm power spectrum

    Science.gov (United States)

    Sarkar, Debanjan; Bharadwaj, Somnath

    2018-05-01

    The post-reionization H I 21-cm signal is an excellent candidate for precision cosmology, this however requires accurate modelling of the expected signal. Sarkar et al. have simulated the real space H I 21-cm signal and have modelled the H I power spectrum as P_{{H I}}(k)=b^2 P(k), where P(k) is the dark matter power spectrum and b(k) is a (possibly complex) scale-dependent bias for which fitting formulas have been provided. This paper extends these simulations to incorporate redshift space distortion and predicts the expected redshift space H I 21-cm power spectrum P^s_{{H I}}(k_{\\perp },k_{allel }) using two different prescriptions for the H I distributions and peculiar velocities. We model P^s_{{H I}}(k_{\\perp },k_{allel }), assuming that it is the product of P_{{H I}}(k)=b^2 P(k) with a Kaiser enhancement term and a Finger of God (FoG) damping which has σp the pair velocity dispersion as a free parameter. Considering several possibilities for the bias and the damping profile, we find that the models with a scale-dependent bias and a Lorentzian damping profile best fit the simulated P^s_{{H I}}(k_{\\perp },k_{allel }) over the entire range 1 ≤ z ≤ 6. The best-fitting value of σp falls approximately as (1 + z)-m with m = 2 and 1.2, respectively, for the two different prescriptions. The model predictions are consistent with the simulations for k models underpredict P^s_2(k) at large k, and the fit is restricted to k < 0.15 Mpc-1.

  16. POST-RELIGION: TRADITIONALISTS’ ANALYSIS

    Directory of Open Access Journals (Sweden)

    Kirill M. Tovbin

    2013-12-01

    Full Text Available Aim of the study is to describe the phenomenon of post-religion as a specific spiritual sphere of the Post-modernity. Methodology. In the introduction analyzed a variety of methodological approaches, opening his inadequacy applied to the spiritual sphere of Post-modernity: «classic» religious studies, religious studies of traditional spiritual movements and the post-modern religious studies, partly produced Traditionalist school, could - according to the author's hypothesis - become a methodological basis for the most complete analysis of post-religion. Scientific novelty. In the main part of the article crystallized the authorized concept of post-religion, its relation with traditional and religious spiritual realms. Post-religion is positioned as the top of secularism enshrined in the departure from the straight and aggressive secularism of the Modernity, but without recourse to Tradition. Post-religion a simulation spirituality, skillfully imitating the outer areas of traditional spirituality and creates a planar religiosity, radically preventing attached to the vertical line of Traditions. In this regard, are the main artificially selected parameters of post-religion: deconstruction, splitting, virtualization, and collage. Deconstruction is a transformation of spirituality in semiotic set for egocentric selectivity of modern believer. Post-religion’s splitting is deprivation of spirituality center, destruction of sacral Center and the transformation of spirituality in the plane on which the intellectual and sensual wandering post-believer from one semiotic island to another. Virtualization is a displacement field of spiritual tension in a completely virtual area, isolated from the natural conditions of existence and created as his replacement, network discussion sites and galleries. Collage is an arbitrary combination of different semiotic pieces of Tradition with the aim of creating a believable picture of tradition; it is collage leads

  17. Post-traumatic Stress Disorder

    Directory of Open Access Journals (Sweden)

    S Seedat

    2013-08-01

    Full Text Available Post-traumatic stress disorder (PTSD is among the most prevalentanxiety disorders, both in terms of lifetime and 12-month prevalencerates documented in epidemiological studies worldwide.

  18. Leukoencephalopaty post chemo and radiotherapy

    International Nuclear Information System (INIS)

    Potente, G.; Iozzino, M.; Amici, A.; Vitturini, A.; Capua, A.; Tombolini, V.

    1987-01-01

    22 patients submitted to radio chemotherapy of the central nervous system have been examined. 6 of them (28%) showed at Ct signs of post radiochemotherapic lekoencephalopathy 4 of them suffered of acute myeloid leukemia and 2 of acute lymphoblastic leukemia

  19. [Post-mortem microbiology analysis].

    Science.gov (United States)

    Fernández-Rodríguez, Amparo; Alberola, Juan; Cohen, Marta Cecilia

    2013-12-01

    Post-mortem microbiology is useful in both clinical and forensic autopsies, and allows a suspected infection to be confirmed. Indeed, it is routinely applied to donor studies in the clinical setting, as well as in sudden and unexpected death in the forensic field. Implementation of specific sampling techniques in autopsy can minimize the possibility of contamination, making interpretation of the results easier. Specific interpretation criteria for post-mortem cultures, the use of molecular diagnosis, and its fusion with molecular biology and histopathology have led to post-mortem microbiology playing a major role in autopsy. Multidisciplinary work involving microbiologists, pathologists, and forensic physicians will help to improve the achievements of post-mortem microbiology, prevent infectious diseases, and contribute to a healthier population. Crown Copyright © 2012. Published by Elsevier Espana. All rights reserved.

  20. Retention and failure morphology of prefabricated posts

    DEFF Research Database (Denmark)

    Sahafi, Alireza; Peutzfeldt, Anne; Asmussen, Erik

    2004-01-01

    PURPOSE: This study evaluated the effect of cement, post material, surface treatment, and shape (1) on the retention of posts luted in the root canals of extracted human teeth and (2) on the failure morphology. MATERIALS AND METHODS: Posts of titanium alloy (ParaPost XH), glass fiber (Para...... at 37 degrees C for 7 days, retention was determined by extraction of the posts. Failure morphology of extracted posts was analyzed and quantified stereomicroscopically. RESULTS: Type of luting cement, post material, and shape of post influenced the retention and failure morphology of the posts. Because...

  1. Post-menopausal breast abscess.

    OpenAIRE

    Raju, G. C.; Naraynsingh, V.; Jankey, N.

    1986-01-01

    Thirty post-menopausal women with breast abscess were treated at Port of Spain General Hospital, Trinidad, between 1976 and 1980. In this age group, breast abscess can be confused with cancer due to a lack of inflammatory features. History and physical examination are often not helpful in differentiating an abscess from carcinoma. Although the usual treatment of an abscess is incision and drainage, in post-menopausal women, excision of the lesion is helpful for accurate histological diagnosis.

  2. Post-menopausal breast abscess.

    Science.gov (United States)

    Raju, G. C.; Naraynsingh, V.; Jankey, N.

    1986-01-01

    Thirty post-menopausal women with breast abscess were treated at Port of Spain General Hospital, Trinidad, between 1976 and 1980. In this age group, breast abscess can be confused with cancer due to a lack of inflammatory features. History and physical examination are often not helpful in differentiating an abscess from carcinoma. Although the usual treatment of an abscess is incision and drainage, in post-menopausal women, excision of the lesion is helpful for accurate histological diagnosis. PMID:3628144

  3. Post-16 update

    Science.gov (United States)

    1999-09-01

    Institute of Physics post-16 initiative, shaping the future Physics education based in IT - sorry ICT! Which, of course, makes for a nifty headline, but as is so often the case with neat slogans, little else. Two formative questions: What, in another decade, will the government of the day call `What you lot should be doing with computers'? and What, of the many things that currently exercise our intellects, will there be anything special to say about in a decade, concerning the use of computers in the teaching of physics? Advancing Physics represents some attempts to come to grips with the second of the two questions above. The first is left to a higher wisdom. In thinking about learning, what can we do with the huge processing power, increasingly available in smaller and less obtrusive packages? What will we do that helps people learn physics, both tomorrow and in 2009? Here are a few suggestions based on development work so far. Wide, reliable and shared access to well ordered learning resources. We have created a CD-ROM, with versions for both student and teacher, that provides a wide range of resources. These do not teach, but do provide. A commitment to allowing a course to evolve and adapt. Electronic publication puts the costs into origination, and not into publication and distribution. A website allows for the community of users to contribute. E-mail networks support individuals and propagate good practice. You can create and explore your own microworlds. The interactive nature of models, and the crafted relationships between the models and the natural world give an insight into the creative imaginary worlds of the physicist. The unreasonable, but pleasurable, success of mathematics in describing the natural world can come to the fore. Measurements that were not possible before are now possible. What was previously indirect, and inaccessible, now becomes a direct measurement, making relationships transparent in new and fruitful ways. The dichotomy between

  4. Post-Newtonian gravitational bremsstrahlung

    International Nuclear Information System (INIS)

    Turner, M.; Will, C.M.

    1978-01-01

    We present formulae and numerical results for the gravitational radiation emitted during a low-deflection encounter between two massive bodies (''gravitational bremsstrahlung''). Our results are valid through post-Newtonian order within general relativity. We discuss in detail the gravitational waveform (transverse-traceless part of the metric perturbation tensor), the toal luminosity and total emitted energy, the angular distribution of emitted energy (antenna pattern), and the frequency spectrum. We also present a method of ''boosting'' the accuracy of these quantities to post-3/2-Newtonian order. A numerical comparison of our results with those of Peters and of Kovacs and Thorne shows that the post-Newtonian method is reliable to better than 0.1% at v=0.1c, to a few percent at v=0.35c, and to 10--20% at v=0.5c. We also compare our results with those of Smarr

  5. Post-Newtonian gravitational bremsstrahlung

    International Nuclear Information System (INIS)

    Turner, M.; Will, C.M.

    1977-07-01

    Formulae and numerical results are presented for the gravitational radiation emitted during a low-deflection encounter between two massive bodies. Results are valid through post-Newtonian order within general relativity. The gravitational waveform, the total luminosity and total emitted energy, the angular distribution of emitted energy, and the frequency spectrum are discussed in detail. A method boosting the accuracy of these quantities to post Newtonian order is also presented. A numerical comparison of results with those of Peters, and of Kovacs and Thorne shows that the post Newtonian method is reliable to better than 0.1 percent at v = 0.1 c, to a few percent at v = 0.35 c, and to 10 to 20 percent at v = 0.5 c

  6. Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland.

    Science.gov (United States)

    Drzewiecka, Dominika; Shashkov, Alexander S; Arbatsky, Nikolay P; Knirel, Yuriy A

    2016-05-01

    A hospitalized 73-year-old woman was infected with a Proteus mirabilis strain, 12 B-r, isolated from the place of injection of a blood catheter. Another strain, 12 B-k, recognized as Proteus genomospecies 5 or 6, was isolated from the patient's faeces, which was an example of a nosocomial infection rather than an auto-infection. Serological investigation using ELISA and Western blotting showed that strain 12 B-k from faeces belonged to the Proteus O2 serogroup. Strain 12 B-r from the wound displayed cross-reactions with several Proteus O serogroups due to common epitopes on the core or O-specific parts of the lipopolysaccharide. Studies of the isolated 12 B-r O-specific polysaccharide by NMR spectroscopy revealed its close structural similarity to that of Proteus O8. The only difference in 12 B-r was the presence of an additional GlcNAc-linked phosphoethanolamine residue, which creates a putative epitope responsible for the cross-reactivity with Pt. mirabilis O16. The new O-antigen form could appear as a result of adaptation of the bacterium to a changing environment. On the basis of the data obtained, we suggest division of the O8 serogroup into two subgroups: O8a for strains of various Proteus species that have been previously classified into the O8 serogroup, and O8a,b for Pt. mirabilis 12 B-r, where 'a' is a common epitope and 'b' is a phosphoethanolamine-associated epitope. These findings further confirm serological and structural heterogeneity of O antigens of Proteus strains isolated lately from patients in Poland.

  7. Post-inflationary brane cosmology

    International Nuclear Information System (INIS)

    Mazumdar, Anupam

    2001-01-01

    The brane cosmology has invoked new challenges to the usual Big Bang cosmology. In this paper we present a brief account on thermal history of the post-inflationary brane cosmology. We have realized that it is not obvious that the post-inflationary brane cosmology would always deviate from the standard Big Bang cosmology. However, if it deviates some stringent conditions on the brane tension are to be satisfied. In this regard we study various implications on gravitino production and its abundance. We discuss Affleck-Dine mechanism for baryogenesis and make some comments on moduli and dilaton problems in this context

  8. Job share a consultant post.

    OpenAIRE

    Thornicroft, G.; Strathdee, G.

    1992-01-01

    Job sharing offers advantages to both employer and employee but it is still uncommon in medicine. Based on the experiences of two psychiatrists sharing a consultant post this article describes some of the problems in obtaining a job share. The most difficult part can be getting an interview, and once a post has been obtained the terms and conditions of service may have to be modified to suit job sharing. Getting on well with your job sharing partner and good communication will not only help o...

  9. Post-heroic warfare revisited

    DEFF Research Database (Denmark)

    Frisk, Kristian

    2017-01-01

    killed during World War II (1940-1945) and the military campaign in Afghanistan (2002-2014), the paper shows that a ‘good’ military death is no longer conceived of as a patriotic sacrifice, but instead legitimised by an appeal to the unique moral worth, humanitarian goals and high professionalism...... of the fallen. The paper concludes that fatalities in transnational military interventions have invoked a sense of post-patriotic heroism instead of a post-heroic crisis, and argues that the social order of modern society has underpinned, rather than undermined, ideals of military self-sacrifice and heroism...

  10. Hydrodynamics of post CHF region

    International Nuclear Information System (INIS)

    Ishii, M.; De Jarlais, G.

    1984-04-01

    Among various two-phase flow regimes, the inverted flow in the post-dryout region is relatively less well understood due to its special heat transfer conditions. The review of existing data indicates further research is needed in the areas of basic hydrodynamics related to liquid core disintegration mechanisms, slug and droplet formations, entrainment, and droplet size distributions. In view of this, the inverted flow is studied in detail both analytically and experimentally. Criteria for initial flow regimes in the post-dryout region are given. Preliminary models for subsequent flow regime transition criteria are derived together with correlations for a mean droplet diameter based on the adiabatic simulation data

  11. Post irradiation examination technology exchange

    International Nuclear Information System (INIS)

    Sozawa, Shizuo; Ito, Masayasu; Taguchi, Taketoshi; Nakagawa, Tetsuya; Lee, Hyung-Kwon

    2012-01-01

    Under the KAERI and JAEA agreement, in a part of the program 18 (Post Irradiation Examination (PIE) and Evaluation Technique of Irradiated Materials), an eddy current test was proposed as a round robin test, and it has been being progressed in both organizations in order to enhance the post irradiation examination technology. Up to now, several data are obtained by both PIE facilities. In this paper, the round robin test program is shown, and also shown obtained data with discussion from applicability as a nondestructive test in the hot cell. (author)

  12. Adding semantics to microblog posts

    NARCIS (Netherlands)

    Meij, E.; Weerkamp, W.; de Rijke, M.

    2012-01-01

    Microblogs have become an important source of information for the purpose of marketing, intelligence, and reputation management. Streams of microblogs are of great value because of their direct and real-time nature. Determining what an individual microblog post is about, however, can be non-trivial

  13. Post-harvest loss reduction

    NARCIS (Netherlands)

    Gogh, van Bart; Boerrigter, Henry; Noordam, Maryvon; Ruben, Ruerd; Timmermans, Toine

    2017-01-01

    This paper was written by experts from Wageningen University & Research (WUR), representing their combined expertise on food chains, post-harvest technology, sustainability, food security, economics, and food safety. The paper was drafted at the request of the Dutch Ministry of Economic Affairs

  14. Chaos Theory and Post Modernism

    Science.gov (United States)

    Snell, Joel

    2009-01-01

    Chaos theory is often associated with post modernism. However, one may make the point that both terms are misunderstood. The point of this article is to define both terms and indicate their relationship. Description: Chaos theory is associated with a definition of a theory dealing with variables (butterflies) that are not directly related to a…

  15. in the Post Colonial State

    African Journals Online (AJOL)

    After nearly 50 years of independence as a nation-state, fierce inter-ethnic competitions for political and ... failure of the post-independence state has failed to permeate the socioeconomic and political lives ...... Northern Ghana", Thesis for the Master of Arts Degree Presented to the McAnulty College and. Graduate School of ...

  16. Post-Exposure Prophylaxis (PEP)

    Science.gov (United States)

    ... Child Transmission of HIV Post-Exposure Prophylaxis (PEP) Pre-Exposure Prophylaxis (PrEP) HIV Treatment HIV Treatment: The Basics Just ... to HIV frequently. Another HIV prevention method, called pre-exposure prophylaxis or PrEP, is when people at high risk ...

  17. Credibility improves topical blog post retrieval

    NARCIS (Netherlands)

    Weerkamp, W.; de Rijke, M.

    2008-01-01

    Topical blog post retrieval is the task of ranking blog posts with respect to their relevance for a given topic. To improve topical blog post retrieval we incorporate textual credibility indicators in the retrieval process. We consider two groups of indicators: post level (determined using

  18. fistules perilymphatiques post-traumatiques post-traumatic ...

    African Journals Online (AJOL)

    (1, 18, 27, 28). CONCLUSION. La fistule péri-lymphatique post-traumatique est de diagnos- tic difficile. Celui-ci est étayé par un faisceau d'arguments cliniques et paracliniques et n'est confirmé qu'en per-opéra- toire. L'indication d'une exploration chirurgicale et son délai dépendent essentiellement de la symptomatologie ...

  19. The post-secular city

    DEFF Research Database (Denmark)

    Greve, Anni

    ) elements in ritual and ceremonies. Second, emergent spaces of post-secular practices are not only connected to traditional religious sites. With Schmautz (1998) it is necessary to understand a sacred site in its original social and cultural setting, and in the present experience of it. With a point...... in research on trans-national identity making (Fortier 2002). This paper suggests a displacement of focus from trans-national to international identity making and the possible role of religious societies. It is argued that an international culture is not mainstream culture however there are (universal......’ the paper argues for a possible potential of storytelling and mutual recognition. Third, it offers an argument for detecting ‘post-secular sites’ at the urban level from two angles: From architecture (the iconic consciousness) and from performance (the sacred drama). Fourth, the paper closes with a case...

  20. Post-Newtonian Jeans Analysis

    International Nuclear Information System (INIS)

    Nazari, Elham; Kazemi, Ali; Roshan, Mahmood; Abbassi, Shahram

    2017-01-01

    The Jeans analysis is studied in the first post-Newtonian limit. In other words, the relativistic effects on local gravitational instability are considered for systems whose characteristic velocities and corresponding gravitational fields are higher than those permitted in the Newtonian limit. The dispersion relation for the propagation of small perturbations is found in the post-Newtonian approximation using two different techniques. A new Jeans mass is derived and compared to the standard Jeans mass. In this limit, the relativistic effects make the new Jeans mass smaller than the Newtonian Jeans mass. Furthermore, the fractional difference between these two masses increases when the temperature/pressure of the system increases. Interestingly, in this limit, pressure can enhance gravitational instability instead of preventing it. Finally, the results are applied to high-temperature astrophysical systems, and the possibility of local fragmentation in some relativistic systems is investigated.

  1. Post-Acquisition IT Integration

    DEFF Research Database (Denmark)

    Henningsson, Stefan; Yetton, Philip

    2013-01-01

    The extant research on post-acquisition IT integration analyzes how acquirers realize IT-based value in individual acquisitions. However, serial acquirers make 60% of acquisitions. These acquisitions are not isolated events, but are components in growth-by-acquisition programs. To explain how...... serial acquirers realize IT-based value, we develop three propositions on the sequential effects on post-acquisition IT integration in acquisition programs. Their combined explanation is that serial acquirers must have a growth-by-acquisition strategy that includes the capability to improve...... IT integration capabilities, to sustain high alignment across acquisitions and to maintain a scalable IT infrastructure with a flat or decreasing cost structure. We begin the process of validating the three propositions by investigating a longitudinal case study of a growth-by-acquisition program....

  2. Post-Newtonian Jeans Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Nazari, Elham; Kazemi, Ali; Roshan, Mahmood; Abbassi, Shahram, E-mail: mroshan@um.ac.ir [Department of Physics, Ferdowsi University of Mashhad, P.O. Box 1436, Mashhad (Iran, Islamic Republic of)

    2017-04-20

    The Jeans analysis is studied in the first post-Newtonian limit. In other words, the relativistic effects on local gravitational instability are considered for systems whose characteristic velocities and corresponding gravitational fields are higher than those permitted in the Newtonian limit. The dispersion relation for the propagation of small perturbations is found in the post-Newtonian approximation using two different techniques. A new Jeans mass is derived and compared to the standard Jeans mass. In this limit, the relativistic effects make the new Jeans mass smaller than the Newtonian Jeans mass. Furthermore, the fractional difference between these two masses increases when the temperature/pressure of the system increases. Interestingly, in this limit, pressure can enhance gravitational instability instead of preventing it. Finally, the results are applied to high-temperature astrophysical systems, and the possibility of local fragmentation in some relativistic systems is investigated.

  3. Testing post-editing guidelines

    DEFF Research Database (Denmark)

    Flanagan, Marian; Christensen, Tina Paulsen

    2014-01-01

    guidelines to use in translator training programmes. Recently, the first set of publicly available industry-focused PE guidelines (for ‘good enough’ and ‘publishable’ quality) were developed by Translation Automation User Society (TAUS) in partnership with the Centre for Global Intelligent Content (CNGL......), which can be used as a basis on which to instruct post-editors in professional environments. This paper reports on a qualitative study that investigates how trainee translators on an MA course, which is aimed at preparing the trainees for the translation industry, interpret these PE guidelines...... for publishable quality. The findings suggest trainees have difficulties interpreting the guidelines, primarily due to trainee competency gaps, but also due to the wording of the guidelines. Based on our findings we propose training measures to address these competency gaps. Furthermore, we provide post...

  4. Post-Digital Literary Studies

    Directory of Open Access Journals (Sweden)

    Florian Cramer

    2016-03-01

    Full Text Available Digital humanities and digital literary studies face much the same challenges as contemporary media art: what will become of them once their media are no longer “new”, and the limitations of processing art as data have become more clearly and widely understood? This paper revisits information aesthetics and computer poetics from the 1960s and 1970s, casting them as precursors of today’s digital humanities, with many of the same issues, achievements and failures, and with their own hype cycles of boom and bust. Conversely, “post-digital” and “Post-Internet” trends in music, graphic design and visual arts may anticipate possible futures of digital humanities and literary studies after the hype has passed.

  5. Post accidental small breaks analysis

    International Nuclear Information System (INIS)

    Depond, G.; Gandrille, J.

    1980-04-01

    EDF ordered to FRAMATOME by 1977 to complete post accidental long term studies on 'First Contrat-Programme' reactors, in order to demonstrate the safety criteria long term compliance, to get information on NSSS behaviour and to improve the post accidental procedures. Convenient analytical models were needed and EDF and FRAMATOME respectively developped the AXEL and FRARELAP codes. The main results of these studies is that for the smallest breaks, it is possible to manually undertake cooling and pressure reducing actions by dumping the steam generators secondary side in order to meet the RHR operating specifications and perform long term cooling through this system. A specific small breaks procedure was written on this basis. The EDF and FRAMATOME codes are continuously improved; the results of a French set of separate effects experiments will be incorporated as well as integral system verification

  6. Post-stroke cognitive impairments

    Directory of Open Access Journals (Sweden)

    Elena Anatolyevna Katunina

    2013-01-01

    Full Text Available Post-stroke cognitive impairments are common effects of stroke. Vascular cognitive impairments are characterized by the heterogeneity of the neuropsychological profile in relation to the site and pattern of stroke. Their common trait is the presence of dysregulation secondary to frontal dysfunction. The treatment of vascular cognitive impairments should be multimodality and aimed at stimulating neuroplasticity processes, restoring neurotransmitter imbalance, and preventing recurrent vascular episodes.

  7. The post-transcriptional operon

    DEFF Research Database (Denmark)

    Tenenbaum, Scott A.; Christiansen, Jan; Nielsen, Henrik

    2011-01-01

    model (PTO) is used to describe data from an assortment of methods (e.g. RIP-Chip, CLIP-Chip, miRNA profiling, ribosome profiling) that globally address the functionality of mRNA. Several examples of post-transcriptional operons have been documented in the literature and demonstrate the usefulness...... of the model in identifying new participants in cellular pathways as well as in deepening our understanding of cellular responses....

  8. The SPOOKI post production system

    Science.gov (United States)

    Beauchemin, M.; Klasa, M.; Fortier, S.; Fortin, F.; Hardy, G.; Pelletier, L.; Edouard, S.; Archambault, B.; Yazidi, H.

    2010-09-01

    The Canadian Meteorological Centre (CMC) delivers a large number of numerical weather prediction products to the various weather offices and clients throughout Canada and abroad. The current post production system was built according to the needs and ideology of the 1980's and it is becoming obsolete with time. Its cumbersome architecture is difficult to maintain and requires a lot of human and computing resources. The "Weather Elements" section of CMC is aware of the problems associated with its maintenance in the long term and has therefore decided to review in depth the whole approach to the operational post production. The analysis of present and future needs have led to the development of an innovative concept in the operational production field inspired by the "Plug and Play" process. SPOOKI (Système de Production Orienté-Objet contennant une Kyrielle d'Informations - Object oriented production system containing a myriad of information) was created in its present form in 2007. It is based on a modular approach where each plug-in component is specialized, reusable and autonomous. These object oriented programming characteristics greatly simplify the maintenance of the system. Particular attention was also given to create a user-friendly system for novice users. An experimental version of SPOOKI is currently running in development mode and an operational one is planned to be implemented in the coming year. The poster presentation will describe SPOOKI, the future CMC operational post production system. Several examples of usage will be shown.

  9. 76 FR 76449 - Post Office Closing

    Science.gov (United States)

    2011-12-07

    ... the closing of the Spring Dale, West Virginia post office has been filed. It identifies preliminary... Postal Service's determination to close the Spring Dale post office in Spring Dale, West Virginia. The...

  10. Post-Conflict Strategic Requirements Workshop

    National Research Council Canada - National Science Library

    Menk, Peter

    2001-01-01

    ... nations and the United Nations. The purpose of the Post-Conflict Strategic Requirements Workshop was to examine the United States military ground forces role in the post-conflict phase of operations...

  11. Post-Flight Data Analysis Tool

    Science.gov (United States)

    George, Marina

    2018-01-01

    A software tool that facilitates the retrieval and analysis of post-flight data. This allows our team and other teams to effectively and efficiently analyze and evaluate post-flight data in order to certify commercial providers.

  12. Issues and Solutions for Command Post Simulations

    National Research Council Canada - National Science Library

    Stytz, Martin R; Banks, Sheila B

    2006-01-01

    .... Unfortunately, the modeling of joint command post teams is still very much in its infancy and this lack of foundational research hinders our ability to assess the performance of command post teams...

  13. 37 CFR 2.81 - Post publication.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Post publication. 2.81 Section 2.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Publication and Post Publication § 2.81 Post publication. (a...

  14. 75 FR 74108 - Post Office Closing

    Science.gov (United States)

    2010-11-30

    ... the closing of the Eugene Post Office's University Station in Eugene, Oregon, has been filed. It... the Eugene Post Office's University Station located in Eugene, Oregon. The petition, which was filed.... Categories of issues apparently raised. The categories of issues raised include: Failure to follow the post...

  15. Imaging Neuroinflammation in Post Traumatic Stress Disorder

    Science.gov (United States)

    2012-11-01

    Post traumatic stress disorder ( PTSD ) is a complex...several central nervous system conditions including post - traumatic stress disorder ( PTSD ) and traumatic brain injury (TBI). Microglia represent over...trials. We have subsequently identified a better agent for interrogating TSPO in post - traumatic stress disorder ( PTSD ) subjects, 18-F PBR111, a

  16. CERN at the Post Office

    CERN Multimedia

    2006-01-01

    In collaboration with CERN and the Conseil général de l'Ain, the French postal services of the Pays de Gex have released a new set of pre-paid envelopes paying tribute to CERN. The envelopes, which come in packets of ten, each featuring a different aspect of CERN, will be on sale from 6 June onwards. They will be available from five post offices in the Pays de Gex (Ferney-Voltaire, Prévessin-Moëns, Saint-Genis-Pouilly, Thoiry and CERN-Prévessin), as well as from the CERN Shop (Reception, Building 33). The envelopes were unveiled at an official ceremony on 23 May, which was attended by Jocelyne Boch, Vice-President of the Conseil général de l'Ain and Mayoress of Thoiry, as well as Marina Combe, Head of the Saint-Genis-Pouilly, Thoiry and CERN-Prévessin post offices, and Maximilian Metzger, CERN's Secretary-General. The last set of envelopes to feature CERN, which commemorated the Laboratory's 50th anniversary in 2004, were a huge success, with sales totalling 35 000 in less than a year. 'We received...

  17. [Post partum depression: future perspectives].

    Science.gov (United States)

    Pinna, Martina; Zompo, Maria Del

    2012-01-01

    Post partum depression (PPD) is a psychiatric illness approximately affecting 10-20% of women after childbirth. The objective of this work is to update our knowledge of PPD giving particular emphasis to etiopathogenetic hypotheses. An accurate search of the literature on this topic was conducted using free dedicated websites such as PubMed. The most recent studies reveal that PPD is a complex disease, whose pathogenesis is not yet clarified, determined by a mix of genetic, biological and environmental factors. Genetic studies have shown a possible involvement of polymorphisms of genes coding for serotonin transporter, 5HT2A and 5HT2C receptors, HMCN1 and METTL13 genes, D2 receptor and GABAA receptor (GABAAR). The involvement of these systems might provide an explanation of the relations among genetic alterations, hormonal fluctuations in the post partum, changes in neurotransmission and mood fluctuations typical of PPD. The results obtained so far are not exhaustive. However, there is a substantial evidence showing that patients with PPD may have a high genetic vulnerability, although we have not been able yet to pinpoint a specific biological marker of the disease. Recent research is focusing on the δ subunit of GABAAR and the possible role of selective agonists of this subunit, such as gaboxadol, in the treatment of PPD.

  18. Parameterized post-Newtonian cosmology

    International Nuclear Information System (INIS)

    Sanghai, Viraj A A; Clifton, Timothy

    2017-01-01

    Einstein’s theory of gravity has been extensively tested on solar system scales, and for isolated astrophysical systems, using the perturbative framework known as the parameterized post-Newtonian (PPN) formalism. This framework is designed for use in the weak-field and slow-motion limit of gravity, and can be used to constrain a large class of metric theories of gravity with data collected from the aforementioned systems. Given the potential of future surveys to probe cosmological scales to high precision, it is a topic of much contemporary interest to construct a similar framework to link Einstein’s theory of gravity and its alternatives to observations on cosmological scales. Our approach to this problem is to adapt and extend the existing PPN formalism for use in cosmology. We derive a set of equations that use the same parameters to consistently model both weak fields and cosmology. This allows us to parameterize a large class of modified theories of gravity and dark energy models on cosmological scales, using just four functions of time. These four functions can be directly linked to the background expansion of the universe, first-order cosmological perturbations, and the weak-field limit of the theory. They also reduce to the standard PPN parameters on solar system scales. We illustrate how dark energy models and scalar-tensor and vector-tensor theories of gravity fit into this framework, which we refer to as ‘parameterized post-Newtonian cosmology’ (PPNC). (paper)

  19. [Post-Lyme disease syndrome].

    Science.gov (United States)

    Błaut-Jurkowska, Justyna; Jurkowski, Marcin

    2016-02-01

    Lyme disease is a chronic infectious disease caused by the bacteria, spirochete of the Borrelia type. Skin, nervous system, musculoskeletal system and heart may be involved in the course of the disease. The prognosis for properly treated Lyme disease is usually good. However, in about 5% of patients so called Post-Lyme disease syndrome (PLSD) develops. It is defined as a syndrome of subjective symptoms persisting despite proper treatment of Borrelia burgdorferi infection. The most common symptoms include: fatigue, muscle and joint pain, and problems with memory and concentration. Pathogenesis of PLDS remains unknown. The differential diagnosis should include neurological, rheumatic and mental diseases. Till now there is no causative treatment of PLDS. In relieving symptom rehabilitation, painkillers, anti-inflammatory and antidepressants medicines are recommended. Emotional and psychological supports are also necessary. Non-specific symptoms reported by patients with post- Lyme disease syndrome raise the suspicion of other pathologies. This can lead to misdiagnosis and implementation of unnecessary, potentially harmful to the patient's therapy. An increase in tick-borne diseases needs to increase physicians awareness of these issues. © 2016 MEDPRESS.

  20. Parameterized post-Newtonian cosmology

    Science.gov (United States)

    Sanghai, Viraj A. A.; Clifton, Timothy

    2017-03-01

    Einstein’s theory of gravity has been extensively tested on solar system scales, and for isolated astrophysical systems, using the perturbative framework known as the parameterized post-Newtonian (PPN) formalism. This framework is designed for use in the weak-field and slow-motion limit of gravity, and can be used to constrain a large class of metric theories of gravity with data collected from the aforementioned systems. Given the potential of future surveys to probe cosmological scales to high precision, it is a topic of much contemporary interest to construct a similar framework to link Einstein’s theory of gravity and its alternatives to observations on cosmological scales. Our approach to this problem is to adapt and extend the existing PPN formalism for use in cosmology. We derive a set of equations that use the same parameters to consistently model both weak fields and cosmology. This allows us to parameterize a large class of modified theories of gravity and dark energy models on cosmological scales, using just four functions of time. These four functions can be directly linked to the background expansion of the universe, first-order cosmological perturbations, and the weak-field limit of the theory. They also reduce to the standard PPN parameters on solar system scales. We illustrate how dark energy models and scalar-tensor and vector-tensor theories of gravity fit into this framework, which we refer to as ‘parameterized post-Newtonian cosmology’ (PPNC).

  1. The Incoherence of Post-Truth

    OpenAIRE

    Taylor, Dom

    2018-01-01

    Ostensibly, there has been a recent rise in ‘post-truth’ thinking (Higgins, 2016; Rochlin, 2017; Speed & Mannion, 2017; Suiter, 2016). The Oxford English Dictionary, which made ‘post-truth’ its word of the year for 2016, defines post-truth as “[r]elating to or denoting circumstances in which objective facts are less influential in shaping political debate or public opinion than appeals to emotion and personal belief” (“Post-truth,” 2017). Going into more detail, post-truth is described not ju...

  2. Subthreshold Post-Traumatic Stress Disorder

    Directory of Open Access Journals (Sweden)

    Eylem Ozten

    2015-12-01

    Full Text Available Post-traumatic stress disorder is a very broad category among mental disorders. Since its inclusion in DSM-III, the diagnostic criteria of post-traumatic stress disorder has undergone a number of changes. The diagnosis and treatment of people who have some symptoms of post-traumatic stress disorder without meeting full criteria still remains controversial. Although subthreshold post-traumatic stress disorder has been debated since it was first defined, the presence of subthreshold post-traumatic stress disorder symptoms has found to raise the risk for suicidal ideation significantly. This article overviews the definitions of trauma related disorders in history of psychiatry and highlights the need to define subthreshold post-traumatic stress disorder symptoms that were reported to be associated with impairment, comorbidity, and suicidal ideation. Clinical differences between subthreshold and full post-traumatic stress disorder will also be discussed.

  3. Post-Secularism, Religious Knowledge and Religious Education

    Science.gov (United States)

    Carr, David

    2012-01-01

    Post-secularism seems to follow in the wake of other (what are here called) "postal" perspectives--post-structuralism, postmodernism, post-empiricism, post-positivism, post-analytical philosophy, post-foundationalism and so on--in questioning or repudiating what it takes to be the epistemic assumptions of "modernism." To be sure, post-secularism…

  4. Post-Communist Welfare Pathways

    DEFF Research Database (Denmark)

    Cerami, Alfio; Vanhuysse, Pieter

    . The authors' impressive analysis of causal factors, including political elites' strategic use of social policy, makes the book an original and important contribution to the comparative welfare state literature.'- Professor Linda J. Cook, Brown University“ 'This edited volume is extraordinarily good...... factors such as micro-causal mechanisms, ideas, discourses, path departures, power politics, and elite strategies. This book includes contributions from leading international Experts such as Claus Offe, Robert Kaufman, Stefan Haggard, Tomasz Inglot, and Mitchell Orenstein, to examine welfare in specific...... countries and across social policy domains. By providing a broad overview based on a theoretical foundation and drawing on recent empirical evidence, Post-Communist Welfare Pathways offers a comprehensive, state-of-the-art account of the progress that has been made since 1989, and the main challenges...

  5. Post-accident radiation monitors

    International Nuclear Information System (INIS)

    Laughlin, G.J.; Kathren, R.L.

    1982-01-01

    Under contract to the Nuclear Safety Analysis Center of the Electric Power Research Institute, technical information and specifications were obtained for commercially available radiological monitoring instrumentation designed for use as post-accident monitors. The information was collated and published in the NSAC Handbook of Postaccident Instrumentation (Kathren and Laughlin 1981), and included such data as range, accuracy, precision, sensitivity, and energy dependence of the detector, environmental and seismic limitations of the equipment, the testing program performed to evaluate the equipment, a list of references where the instrumentation is currently installed, and a list of features and accessories available with the monitoring systems. The information presented in this section reveals that, even though a number of vendors claim to be able to meet the guidance of Regulatory Guide 1.97 (USNRC 1980), few have actually conducted tests to verify that their equipment does indeed satisfy the guidance of this Regulatory Guide, and that some of the guidance may in fact be unrealistic

  6. Leg ischemia post-varicocelectomy

    Directory of Open Access Journals (Sweden)

    Al-Wahbi AM

    2016-03-01

    Full Text Available Abdullah M Al-Wahbi1, Shaza Elmoukaied2 1Division of Vascular Surgery, Department of Surgery, King Abdulaziz Medical City, Riyadh, Saudi Arabia; 2Department of Surgery, Dr Sulaiman Al Habib Hospital, Riyadh, Saudi Arabia Abstract: Varicocelectomy is the most commonly performed operation for the treatment of male infertility. Many surgical approaches are used as each of them has advantages over the other and is preferred by surgeons. Vascular injury has never been reported as a complication of varicocelectomy apart from testicular artery injury. We present a 36-year-old male who developed leg ischemia post-varicocelectomy due to common femoral artery injury. He was successfully treated by using a vein graft. Keywords: varicocele, varicocelectomy, complications, vascular injuries

  7. Post-Industrial Cultural Criticism

    DEFF Research Database (Denmark)

    Kammer, Aske

    2015-01-01

    hierarchies within journalism. The article maps which Danish websites conduct arts and culture reviews, asks what features these websites have that facilitate public discourse, and measures the actual discussion on the websites. While academic diagnoses of the state of the online public sphere have generally......Integrating perspectives from research into cultural and post-industrial journalism, this article presents a pilot study of websites with reviews of arts and culture conducted by amateurs. Such websites constitute a popular space for cultural criticism, and one that challenges traditional...... reviewers have highly specialized knowledge of culture and, on that basis, argues that the emergence of this type of critic might represent a qualitative strengthening of cultural criticism....

  8. Successful Pregnancies Post Renal Transplantation

    Directory of Open Access Journals (Sweden)

    Alfi Adnan

    2008-01-01

    Full Text Available To evaluate the maternal and fetal outcomes in renal transplant female recipients who became pregnant from 1989 to 2005 in our center, we retrospectively studied 20 incident pregnancies in 12 renal transplant recipients; 5 (41.7 % of them from living related, 4 (33.3% from deceased, and 3 (25% from living unrelated donors. The mean age at pregnancy was 30.5 ± 4.5 years and mean interval from transplantation to pregnancy was 21 ± 5.7 months with the interval was < 1 year in one patient. The mean serum creatinine (SCr before pregnancy vs 6 months post delivery was 110 ± 24.3, and 156 ± 190 µmol/ L, respectively, (p = 0.2. All patients were normotensive during the prenatal period except two who were hypertensive, none was markedly proteinuric, and only one acute rejection episode occurred during one pregnancy. Graft loss one year post delivery occurred in 2 patients; one with elevated prenatal SCr > 132 µmol/L, and another with short interval from transplantation to pregnancy < 1 year, while the remaining 10 patients revealed current mean SCr of 105 ± 18.2 µmol/L. Complications during pregnancy inclu-ded pre-eclampsia in (25%, UTI (25%, preterm delivery < 37 weeks (30%, however, none of the pregnancies ended by abortion. Normal vaginal delivery vs cesarean section was 70% vs 30%, respectively. Gestational age at delivery was 36.3 ± 3.9 weeks, and mean fetal birth weight was 2349 ± 574 gm. Apgar score was 9-10 in all of the 20 babies, and none revealed intrauterine growth retardation or congenital anomalies. We conclude that consecutive pregnancies demons-trate long-term maternal and fetal survival and function. The major risk factors are elevated starting serum creatinine, hypertension, and short time interval from transplantation to pregnancy.

  9. Post-therapeutic imaging findings

    Energy Technology Data Exchange (ETDEWEB)

    Pollice, Saverio, E-mail: saveriopollice@hotmail.it [Department of Radiology and Neuroradiology, “L. Bonomo Hospital”, 76123 Andria, BT (Italy); Muto, Mario, E-mail: mutomar@tiscali.it [Department of Neuroradiology, “Cardarelli Hospital”, Naples (Italy); Scarabino, Tommaso, E-mail: tscarabino@hotmail.com [Department of Radiology and Neuroradiology, “L. Bonomo Hospital”, 76123 Andria, BT (Italy)

    2015-05-15

    Highlights: • This study is the result of collaboration between neuroradiologists and neurosurgeons. • Comparison between neuroimaging techniques to better evaluate the complications after treatment of the spin. • Evaluation of the imaging features of complications and definition of follow-up. - Abstract: Any surgical approach modifies the normal anatomical and functional arrangement of the segmental spine which is aimed, therefore image interpretation cannot ignore a correct set of knowledge in the field of anatomy, pathophysiology, drug compliance, interventional radiology and surgery. Neuroradiological imaging has an important role before surgery to direct the surgeon or interventional radiologist during the operation, both in post-surgery, where imaging examination can rightly evaluate properties and effects of the treatment and can detect potential complications as infections, abscess, bleeding, exuberant scar, mobilization and rupture of devices. The available methods of imaging are the X-rays (XR) made at least in two projections, Computed Tomography (CT) with MPR (multiplanar) and VR (volume rendering) reconstruction, and Magnetic Resonance (MR), often performed before and after contrast media injection. Imaging assessment of spine after surgery is complex and depends upon several factors, including surgical procedures and disease for which it was performed; biomechanical of the underlying cortical and cancellous bone findings; conditions of muscles, intervertebral disk and ligaments; time since surgery procedures; duration and nature of the post-surgical syndrome. Depending upon several factors, one or a combination of complementary imaging modalities (X-rays, Computed Tomography, Magnetic Resonance) may be required to evaluate effectiveness of the treatment; to demonstrate any clinically relevant abnormality at the treated region and adjacent structures (complications such as inflammation, abscesses, bleeding and misplacement of the device); to

  10. Post-therapeutic imaging findings

    International Nuclear Information System (INIS)

    Pollice, Saverio; Muto, Mario; Scarabino, Tommaso

    2015-01-01

    Highlights: • This study is the result of collaboration between neuroradiologists and neurosurgeons. • Comparison between neuroimaging techniques to better evaluate the complications after treatment of the spin. • Evaluation of the imaging features of complications and definition of follow-up. - Abstract: Any surgical approach modifies the normal anatomical and functional arrangement of the segmental spine which is aimed, therefore image interpretation cannot ignore a correct set of knowledge in the field of anatomy, pathophysiology, drug compliance, interventional radiology and surgery. Neuroradiological imaging has an important role before surgery to direct the surgeon or interventional radiologist during the operation, both in post-surgery, where imaging examination can rightly evaluate properties and effects of the treatment and can detect potential complications as infections, abscess, bleeding, exuberant scar, mobilization and rupture of devices. The available methods of imaging are the X-rays (XR) made at least in two projections, Computed Tomography (CT) with MPR (multiplanar) and VR (volume rendering) reconstruction, and Magnetic Resonance (MR), often performed before and after contrast media injection. Imaging assessment of spine after surgery is complex and depends upon several factors, including surgical procedures and disease for which it was performed; biomechanical of the underlying cortical and cancellous bone findings; conditions of muscles, intervertebral disk and ligaments; time since surgery procedures; duration and nature of the post-surgical syndrome. Depending upon several factors, one or a combination of complementary imaging modalities (X-rays, Computed Tomography, Magnetic Resonance) may be required to evaluate effectiveness of the treatment; to demonstrate any clinically relevant abnormality at the treated region and adjacent structures (complications such as inflammation, abscesses, bleeding and misplacement of the device); to

  11. Management Of Post Stroke Seizures

    Directory of Open Access Journals (Sweden)

    Kavian Ghandehari

    2017-02-01

    Full Text Available The incidence of seizures in relation to stroke is 8.9%, with a frequency of 10.6 and 8.6% in haemorrhagic and ischaemic stroke, respectively. In subarachnoid haemorrhage the incidence is 8.5%. Due to the fact that infarcts are significantly more frequent than haemorrhages, seizures are mainly related to occlusive vascular disease of the brain. The general view is to consider stroke-related seizures as harmless complications in the course of a prolonged vascular disease involving the heart and brain. Seizures can be classified as those of early and those of late onset in a paradigm comparable to post-traumatic epilepsy, with an arbitrary dividing point of two weeks after the event. Most early-onset seizures occur during the first day after the stroke. Late-onset seizures occur three times more often than early-onset ones. A first late-onset epileptic event is most likely to take place between six months and two years after the stroke. However, up to 28% of patients develop their first seizure several years later. Simple partial seizures, with or without secondary generalisation, account for about 50% of total seizures, while complex partial spells, with or without secondary generalisation, and primary generalised tonic–clonic insults account for approximately 25% each. Status epilepticus occurs in 12% of stroke patients, but the recurrence rate after an initial status epilepticus is not higher than after a single seizure. Inhibitory seizures, mimicking transient ischaemic attacks, are observed in 7.1% of cases. The only clinical predictor of late-onset seizures is the initial presentation of partial anterior circulation syndrome due to a territorial infarct. Patients with total anterior circulation syndrome have less chance of developing epileptic spells, not only due to their shorter life expectancy but also due to the fact that the large infarcts are sharply demarcated in these patients. The optimal timing and type of antiepileptic drug

  12. Gynecologic Malignancies Post-LeFort Colpocleisis

    Directory of Open Access Journals (Sweden)

    Rayan Elkattah

    2014-01-01

    Full Text Available Introduction. LeFort colpocleisis (LFC is a safe and effective obliterative surgical option for older women with advanced pelvic organ prolapse who no longer desire coital activity. A major disadvantage is the limited ability to evaluate for post-LFC gynecologic malignancies. Methods. We present the first case of endometrioid ovarian cancer diagnosed after LFC and review all reported gynecologic malignancies post-LFC in the English medical literature. Results. This is the second reported ovarian cancer post-LFC and the first of the endometrioid subtype. A total of nine other gynecologic malignancies post-LFC have been reported in the English medical literature. Conclusions. Gynecologic malignancies post-LFC are rare. We propose a simple 3-step strategy in evaluating post-LFC malignancies.

  13. Development of new radiopaque glass fiber posts

    International Nuclear Information System (INIS)

    Furtos, Gabriel; Baldea, Bogdan; Silaghi-Dumitrescu, Laura

    2016-01-01

    The aim of this study was to analyze the radiopacity and filler content of three experimental glass fiber posts (EGFP) in comparison with other glass/carbon fibers and metal posts from the dental market. Three EGFP were obtained by pultrusion of glass fibers in a polymer matrix based on 2,2-bis[4-(2-hydroxy-3-methacryloyloxypropoxy)-phenyl]propane (bis-GMA) and triethyleneglycol dimethacrylate (TEGDMA) monomers. Using intraoral sensor disks 27 posts, as well as mesiodistal sections of human molar and aluminum step wedges were radiographed for evaluation of radiopacity. The percentage compositions of fillers by weight and volume were investigated by combustion analysis. Two EGFP showed radiopacity higher than enamel. The commercial endodontic posts showed radiopacity as follows: higher than enamel, between enamel and dentin, and lower than dentin. The results showed statistically significant differences (p < 0.05) when evaluated with one-way ANOVA statistical analysis. According to combustion analyses, the filler content of the tested posts ranges between 58.84 wt.% and 86.02 wt.%. The filler content of the tested EGFP ranged between 68.91 wt.% and 79.04 wt.%. EGFP could be an alternative to commercial glass fiber posts. Future glass fiber posts are recommended to present higher radiopacity than dentin and perhaps ideally similar to or higher than that of enamel, for improved clinical detection. The posts with a lower radiopacity than dentin should be considered insufficiently radiopaque. The radiopacity of some glass fiber posts is not greatly influenced by the amount of filler. - Highlights: • AR glass fibers for dental applications • AR glass fibers have a great potential for obtaining radiopaque glass fiber posts. • Experimental AR glass fiber posts could be an alternative to commercial glass fiber posts for clinical application.

  14. Development of new radiopaque glass fiber posts

    Energy Technology Data Exchange (ETDEWEB)

    Furtos, Gabriel, E-mail: gfurtos@yahoo.co.uk [Raluca Ripan Institute of Research in Chemistry, Babes-Bolyai University, Cluj-Napoca (Romania); Baldea, Bogdan [Dep. of Prosthodontics, Faculty of Dental Medicine, Timisoara (Romania); Silaghi-Dumitrescu, Laura [Raluca Ripan Institute of Research in Chemistry, Babes-Bolyai University, Cluj-Napoca (Romania)

    2016-02-01

    The aim of this study was to analyze the radiopacity and filler content of three experimental glass fiber posts (EGFP) in comparison with other glass/carbon fibers and metal posts from the dental market. Three EGFP were obtained by pultrusion of glass fibers in a polymer matrix based on 2,2-bis[4-(2-hydroxy-3-methacryloyloxypropoxy)-phenyl]propane (bis-GMA) and triethyleneglycol dimethacrylate (TEGDMA) monomers. Using intraoral sensor disks 27 posts, as well as mesiodistal sections of human molar and aluminum step wedges were radiographed for evaluation of radiopacity. The percentage compositions of fillers by weight and volume were investigated by combustion analysis. Two EGFP showed radiopacity higher than enamel. The commercial endodontic posts showed radiopacity as follows: higher than enamel, between enamel and dentin, and lower than dentin. The results showed statistically significant differences (p < 0.05) when evaluated with one-way ANOVA statistical analysis. According to combustion analyses, the filler content of the tested posts ranges between 58.84 wt.% and 86.02 wt.%. The filler content of the tested EGFP ranged between 68.91 wt.% and 79.04 wt.%. EGFP could be an alternative to commercial glass fiber posts. Future glass fiber posts are recommended to present higher radiopacity than dentin and perhaps ideally similar to or higher than that of enamel, for improved clinical detection. The posts with a lower radiopacity than dentin should be considered insufficiently radiopaque. The radiopacity of some glass fiber posts is not greatly influenced by the amount of filler. - Highlights: • AR glass fibers for dental applications • AR glass fibers have a great potential for obtaining radiopaque glass fiber posts. • Experimental AR glass fiber posts could be an alternative to commercial glass fiber posts for clinical application.

  15. [Post-traumatic stress disorder after childbirth].

    Science.gov (United States)

    Korábová, I; Masopustová, Z

    2016-01-01

    The aim of this paper is to introduce the issue of post-traumatic stress disorder after childbirth to health care professionals. The text focuses on the diagnostic definition of post-traumatic stress disorder after childbirth, symptoms, physiological background, prevalence, course, risk factors and consequences of post-traumatic stress disorder after childbirth for a woman, her child and her partner. Options for interventions and therapy are outlined as well.

  16. Catecholamines in Post-Traumatic Stress Disorder

    Science.gov (United States)

    2012-07-01

    CONTRACT NUMBER Catecholamines in post - traumatic stress disorder 5b. GRANT NUMBER W81XWH-08-1-0327 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d...emotionally arousing experiences are typically vivid and persistent. The recurrent, intrusive memories of traumatic events in post - traumatic stress disorder ...AD_________________ Award Number: W81XWH-08-1-0327 TITLE: Catecholamines in post - traumatic stress

  17. Post spinal meningitis and asepsis.

    Science.gov (United States)

    Videira, Rogerio L R; Ruiz-Neto, P P; Brandao Neto, M

    2002-07-01

    Post spinal meningitis (PSM) is a complication still currently being reported. After two PSM cases in our hospital an epidemiological study was initiated, which included a survey of techniques for asepsis that are applied in our department. Cases defined as PSM comprised meningitis within a week after spinal anesthesia. Anesthesia records, anesthesia complication files and the records of the Hospital Commission for Infection Control from 1997 to 2000 were reviewed. Asepsis techniques applied were surveyed by a questionnaire answered by all our department's anesthesiologists. The equipment and procedures for spinal anesthesia were listed. Current anesthesia textbooks were reviewed for recommendations regarding asepsis techniques in conjunction with spinal anesthesia. Three cases of PSM were identified following 38,128 spinal anesthesias whereas none was observed in 12,822 patients subjected to other types of regional or general anesthesia (P>0.05). Culture of cerebrospinal fluid yielded Streptococcus in two patients and was negative in the other patient. The asepsis technique applied by the anesthesiologists varied considerably. The literature review showed that aspects on asepsis for spinal anesthesia are poorly covered. The incidence of meningitis was similar in patients subjected to spinal anesthesia and in those subjected to other anesthetic techniques. Asepsis techniques were found to differ considerably among our staff members, reflecting the lack of well-defined published standards for this procedure. We recommend that asepsis for spinal anesthesia should not be less rigorous than for surgical asepsis.

  18. STS-70 Post Flight Presentation

    Science.gov (United States)

    Peterson, Glen (Editor)

    1995-01-01

    In this post-flight overview, the flight crew of the STS-70 mission, Tom Henricks (Cmdr.), Kevin Kregel (Pilot), Major Nancy Currie (MS), Dr. Mary Ellen Weber (MS), and Dr. Don Thomas (MS), discuss their mission and accompanying experiments. Pre-flight, launch, and orbital footage is followed by the in-orbit deployment of the Tracking and Data Relay Satellite (TDRS) and a discussion of the following spaceborne experiments: a microgravity bioreactor experiment to grow 3D body-like tissue; pregnant rat muscular changes in microgravity; embryonic development in microgravity; Shuttle Amateur Radio Experiment (SAREX); terrain surface imagery using the HERCULES camera; and a range of other physiological tests, including an eye and vision test. Views of Earth include: tropical storm Chantal; the Nile River and Red Sea; lightning over Brazil. A three planet view (Earth, Mars, and Venus) was taken right before sunrise. The end footage shows shuttle pre-landing checkout, entry, and landing, along with a slide presentation of the flight.

  19. Post-crisis financial intermediation

    Directory of Open Access Journals (Sweden)

    Ilie MIHAI

    2015-09-01

    Full Text Available The recent financial crisis that begun in 2007 in the US, which then swept around the world, has left deep scars on the already wrinkled face of the global economy. Some national and regional economies, which had money for expensive makeup, or created money[1], managed to blur or hide the scars left by the crisis, others are still facing difficulties in overcoming the effects of this. The rapacity of banks, their greed and risk ignorance, were the origin of the outbreak of the last major economic and financial crisis but unfortunately those who were responsible or, rather, irresponsible, paid little or nothing at all for the burden of their bad loan portfolio. This cost has been supported by the population, either directly by paying high interest and fees [Mihai I., 2007], or indirectly, through the use of public budgets to cover the losses of banks, most of which had private capital. In this context, we intend to examine the state of financial intermediation in Romania in the post-crisis period, and to primarily follow: (i The structure and evolution of the banking system; (ii Non-government credit situation; (iii The level of savings; (iiii Loan-deposit ratio; (v The degree of financial intermediation and disintegration phenomenon etc., and to articulate some conclusions and suggestions on the matters that have been explored.

  20. Post-mining in France

    International Nuclear Information System (INIS)

    2007-01-01

    This plentifully illustrated book aims at showing how new equilibria are building up during the transition between mining activity and post-mining, and at stressing on the necessity to keep up the cultural elements, the competencies and knowledge of mining works. The first chapter - mine and men - shows the importance of mineral substances in the objects of the everyday life, illustrates the importance of the mining tradition in France and describes the technical and administrative organisation of the end of the mining activity (works, rehabilitation, regulation, monitoring..). Chapter two - exploitation methods - presents the surface and underground facilities and their impact on the environment (extraction machines, workshops, ore processing plants, decantation ponds..). The third chapter deals with the rehabilitation and monitoring aspects: impact of mining activity stoppage on underground and surface waters, land stability, soils cleansing.. The last chapter summarizes the history of French mining region by region: Nord-Pas-de-Calais, Lorraine-Alsace, Massif central, Bretagne-Normandie, Provence-Alpes-Cote d'Azur and Pyrenees

  1. STS 63: Post flight presentation

    Science.gov (United States)

    1995-02-01

    At a post flight conference, Captain Jim Wetherbee, of STS Flight 63, introduces each of the other members of the STS 63 crew (Eileen Collins, Pilot; Dr. Bernard Harris, Payload Commander; Dr. Michael Foale, Mission Specialist from England; Dr. Janice Voss, Mission Specialist; and Colonel Vladimir Titor, Mission Specialist from Russia), gave a short autobiography of each member and a brief description of their assignment during this mission. A film was shown that included the preflight suit-up, a view of the launch site, the actual night launch, a tour of the Space Shuttle and several of the experiment areas, several views of earth and the MIR Space Station and cosmonauts, the MlR-Space Shuttle rendezvous, the deployment of the Spartan Ultraviolet Telescope, Foale and Harris's EVA and space walk, the retrieval of Spartan, and the night entry home, including the landing. Several spaceborne experiments were introduced: the radiation monitoring experiment, environment monitoring experiment, solid surface combustion experiment, and protein crystal growth and plant growth experiments. This conference ended with still, color pictures, taken by the astronauts during the entire STS 63 flight, being shown.

  2. Post-transplant lymphoproliferative disorders.

    Science.gov (United States)

    Singavi, Arun K; Harrington, Alexandra M; Fenske, Timothy S

    2015-01-01

    Post-transplant lymphoproliferative disorders (PTLD) are a serious complication after solid organ or allogeneic hematopoietic stem cell transplantation and include a range of diseases from benign proliferations to malignant lymphomas. Risk factors for developing PTLD include Epstein-Barr virus (EBV) infection, recipient age, transplanted organ, type of immunosuppression, and genetics. Uncontrolled proliferation of EBV-infected B cells is implicated in EBV-positive PTLD, whereas the pathogenesis of EBV-negative PTLD may be similar to non-Hodgkin's lymphoma in the general population. The World Health Organization (WHO) classifies PTLD into four categories: early lesions, polymorphic PTLD, monomorphic PTLD, and classical Hodgkin's lymphoma (cHL). Treatment is aimed at cure of PTLD, while maintaining transplanted organ function. However, there are no established guidelines for the treatment of PTLD. Immune suppression reduction (ISR) is the first line of treatment in most cases, with more recent data suggesting early use of rituximab. In more aggressive forms of PTLD, upfront chemotherapy may offer a better and more durable response. Sequential therapy using rituximab followed by chemotherapy has demonstrated promising results and may establish a standard of care. Novel therapies including anti-viral agents, adoptive immunotherapy, and monoclonal antibodies targeting cytokines require further study in the prevention and treatment of PTLD.

  3. Post Pelvic Radiotherapy Bony Changes

    Energy Technology Data Exchange (ETDEWEB)

    Huh, Seung Jae [Samsung Medical Center, Seoul (Korea, Republic of)

    2009-03-15

    There has been recent interest in radiation-induced bone injury in clinical conditions, especially for pelvic insufficiency fracture (PIF). A PIF is caused by the effect of normal or physiological stress on bone with demineralization and decreased elastic resistance. Pelvic radiotherapy (RT) can also contribute to the development of a PIF. A PIF has been regarded as a rare complication with the use of megavoltage equipment. However, recent studies have reported the incidence of PIFs as 8.2{approx}20% after pelvic RT in gynecological patients, an incidence that was higher than previously believed. The importance of understanding a PIF lies in the potential for misdiagnosis as a bony metastasis. If patients complain of pelvic pain after whole-pelvis radiation therapy, the presence of a PIF must be considered in the differential diagnosis. The use of multibeam arrangements and conformal RT to reduce the volume and dose of irradiated pelvic bone can be helpful to minimize the risk of fracture. In addition to a PIF, osteonecrosis and avascular necrosis of the femoral head can develop after radiation therapy. Osteoradionecrosis of the pelvic bone is a clinical diagnostic challenge that must be differentiated from an osseous metastasis. A post-radiation bone sarcoma can result as a long-term sequela of pelvic irradiation for uterine cervical cancer.

  4. Post Implementation Review Framework and Procedures

    Data.gov (United States)

    Social Security Administration — This template outlines the Social Security Administration's (SSA) approach to initiating, conducting, and completing Post Implementation Reviews (PIRs). The template...

  5. A Review of Post and Core Application with Emphasize on Non Metallic Posts

    Directory of Open Access Journals (Sweden)

    Shahroodi MH

    2001-05-01

    Full Text Available Many different methods are suggested to restore endodontically treated teeth. Prefabricated posts can not be indicated for all teeth and cast posts require extra time and cost. In addition, with the introduction of full ceramic restorations, achieving the ideal esthetic with metal post underneath them may be problematic or impossible because the darkness of the metallic posts may show through the highly translucent all ceramic restorations. In this article the review of litature and describiton of applied methods of different procedure in restoring the root canal therapied teeth and few techniques of non metallic posts fabrication such as fiber reinforced composite and zirconium oxide posts have been described.

  6. Microleakage of Different Post Systems and a Custom Adapted Fiber Post

    Directory of Open Access Journals (Sweden)

    Farideh Geramipanah

    2013-01-01

    Full Text Available Objective: The effects of closely adapting a prefabricated fiber to the post space remain unknown. The purpose of this study was to quantify the microleakages of a custom adapted fiber-reinforced post, a prefabricated quartz fiber post and a cast post using nondestructive methods.Materials and Methods: Sixty-five extracted human premolars were endodontically treated and randomly divided into three groups (n=15, which were restored using a cast post-and-core, a custom adapted fiber post (Refropost with a microhybrid microfiller resin composite (Gradia, or a prefabricated quartz fiber post (DT light post and two groups of control ( n=10. All groups were cemented using a dual polymerizing resin cement (Panavia F2.0. A composite core (Z100 was used for the fiber posts. The microleakage was calculated for the experimental and control groups before and after thermal cycling and cycling loading using a radiotracer solution (thallium 201 chloride and a gamma counter device. Data were subjected to statistical analysis of ANOVA and Tukey HSD at significant level of P< 0.05.Results: Significantly lower microleakage values were found for the cast post-and-core (mean value =16.04 ×104 and custom adapted fiber post groups (mean value=14.36×104. Thermal cycling and cyclic loading had no significant effect on the microleakage value of any tested group.Conclusion: Post systems with improved adaptation showed similar microleakage to casting post

  7. Post-Retrofit Residential Assessments

    Energy Technology Data Exchange (ETDEWEB)

    Lancaster, Ross; lutzenhiser, Loren; Moezzi, Mithra; Widder, Sarah H.; Chandra, Subrato; Baechler, Michael C.

    2012-04-30

    This study examined a range of factors influencing energy consumption in households that had participated in residential energy-efficiency upgrades. The study was funded by a grant from the U.S. Department of Energy’s Pacific Northwest National Laboratory and was conducted by faculty and staff of Portland State University Center for Urban Studies and Department of Economics. This work was made possible through the assistance and support of the Energy Trust of Oregon (ETO), whose residential energy-efficiency programs provided the population from which the sample cases were drawn. All households in the study had participated in the ETO Home Performance with Energy Star (HPwES) program. A number of these had concurrently pursued measures through other ETO programs. Post-retrofit energy outcomes are rarely investigated on a house-by-house basis. Rather, aggregate changes are ordinarily the focus of program impact evaluations, with deviation from aggregate expectations chalked up to measurement error, the vagaries of weather and idiosyncrasies of occupants. However, understanding how homes perform post-retrofit on an individual basis can give important insights to increase energy savings at the participant and the programmatic level. Taking a more disaggregated approach, this study analyzed energy consumption data from before and after the retrofit activity and made comparisons with engineering estimates for the upgrades, to identify households that performed differently from what may have been expected based on the estimates. A statistical analysis using hierarchal linear models, which accounted for weather variations, was performed looking separately at gas and electrical use during the periods before and after upgrades took place. A more straightforward comparison of billing data for 12-month periods before and after the intervention was also performed, yielding the majority of the cases examined. The later approach allowed total energy use and costs to be

  8. Diagnostic Uncertainties in Post-stroke Pain

    NARCIS (Netherlands)

    Roosink, M.; Renzenbrink, G.J.; Van Dongen, R.T.M.; Buitenweg, Jan R.; Geurts, A.C.H.; IJzerman, Maarten Joost

    2008-01-01

    Aim of Investigation Pain is a common complication after stroke. The etiology of post-stroke pain is largely unknown and classification of post-stroke pain subtypes is primarily based on neurological examination and pain assessment. Classification could probably be improved by a better understanding

  9. Post-Colonialism Perspectives on Educational Competition

    Science.gov (United States)

    Yeh, Chuan-Rong

    2016-01-01

    Educational competition has always been the puzzle issue of educational researches. In this article, I analyze several aspects of educational competition within the perspective of post-colonialism discourse. In the political aspect, Taiwanese education is linked with political power, to present the post-colonial spirit by continuing dynastic…

  10. A Short Course in Post-Structuralism.

    Science.gov (United States)

    Tompkins, Jane

    1988-01-01

    Asserts that post-structuralism cannot be applied to literary texts because to talk about applying post-structuralism assumes: (1) free-standing subjects; (2) free-standing objects of investigation; (3) free-standing methods; and (4) free-standing interpretation. (RAE)

  11. 76 FR 67498 - Post Office Closing

    Science.gov (United States)

    2011-11-01

    ... POSTAL REGULATORY COMMISSION [Docket No. A2012-17; Order No. 918] Post Office Closing AGENCY: Postal Regulatory Commission. ACTION: Notice. SUMMARY: This document informs the public that an appeal of the closing of the Venice, California post office has been filed. It identifies preliminary steps and...

  12. Posting of workers : Enforcement problems and challenges

    NARCIS (Netherlands)

    Cremers, Jan

    2013-01-01

    Expert contribution to a final conference. According to the author, monitoring the process of posting and controlling the regular character of the cross-border provision of services with posted workers has proven to be a problematic area. Recent joint activities of the labour inspectorate have

  13. [In vivo evaluation of carbon fiber posts].

    Science.gov (United States)

    Lai, V; Lugliè, P F; Chessa, G

    2002-05-01

    The use of carbon fiber posts allows morpho-functional restoration of endodontically treated teeth with an assembly of materials of a modulus of elasticity similar to that of dentin. The study clinically evaluated the percentage of survival of dental elements treated and reconstructed with endocanal carbon fiber posts. At the Dentistry Clinic of the University of Sassari 60 dental elements were selected from 46 subjects. The teeth, which had been treated endodontically with success for at least six months, were classified by parameters taken from the international literature and reconstructed using Tech 2000 carbon fiber posts and adhesive resinous systems recommended by the post manufacturer. The success rate was 98.4%. Almost half (49%) of the samples were single-rooted elements, 37.4% of the posts were 1.2 mm in diameter; in 78.3% the opposing contact was with a natural tooth; 100% of the elements had a type A dental structure. Third generation posts are a valid alternative to metallic posts and improve the prognosis of the treated element. The carbon fiber posts fixed with the composite, forming a single unit with the dental element, thus improving mid-term RESULTS. The technique is easy to use under clinical conditions and can be performed in a single session. So far, the method has provided promising clinical results, as this study demonstrated.

  14. Patients' quality of life post thyroidectomy.

    LENUS (Irish Health Repository)

    Cashman, E C

    2011-01-01

    This study was designed to evaluate health related quality of life post thyroidectomy for hyperthyroidism with respect to clinical benefit and patient satisfaction. This is one of the first such studies in the literature evaluating quality of life post thyroidectomy for hyperthyroidism.

  15. Post-tensioning system surveillance program

    International Nuclear Information System (INIS)

    Drew, G.E.

    1979-01-01

    Nuclear power plant containment structure post-tensioning system tendon surveillance program is described in detail. Data collected over three yearly post-tensioning system Surveillance Programs is presented and evaluated to correlate anticipated stress losses with actual losses. In addition corrosion protected system performance is analyzed

  16. Post-Modern Perspectives on Orthodox Positivism

    NARCIS (Netherlands)

    Venzke, I.

    2013-01-01

    This contribution explains the travails of international legal positivism (ILP) from post-modern perspectives. It identifies conventional precepts of orthodox ILP and shows how variants of post-modern thinking unravel them. The focus rests on three main such precepts and their critique: first,

  17. Managing Post-Disaster Needs Assessments (PDNA)

    OpenAIRE

    Jones, Brett

    2010-01-01

    This knowledge note provides an overview of the post-disaster assessment process, extracting lessons learned in the East Asia Pacific Region (EAP) and presenting best practices from recent assessments. The note explains the Post-Disaster Needs Assessment (PDNA) methodology, and outlines: (i) the assessment triggers, (ii) key steps in assessment planning, and (iii) dos and don'ts in assessm...

  18. Comparison of transcriptomic signature of post-Chernobyl and post radiotherapy thyroid tumors

    International Nuclear Information System (INIS)

    Ory, Catherine; Ugolin, Nicolas; Chevillard, Sylvie; Hofman, Paul; Schlumberger, Martin; Likhtarev, Illya A.

    2013-01-01

    We previously identified two highly discriminating and predictive radiation-induced transcriptomic signatures by comparing series of sporadic and post radiotherapy thyroid tumors (322-gene signature), and by reanalyzing a previously published data set of sporadic and post-Chernobyl thyroid tumors (106-gene signature). The aim of the present work was (i) to compare the two signatures in terms of gene expression de-regulations and molecular features/pathways, and (ii) to test the capacity of the post radiotherapy signature in classifying the post-Chernobyl series of tumors and reciprocally of the post-Chernobyl signature in classifying the post radiotherapy-induced tumors. We now explored if post radiotherapy and post-Chernobyl papillary thyroid carcinomas (PTC) display common molecular features by comparing molecular pathways deregulated in the two tumor series, and tested the potential of gene subsets of the post radiotherapy signature to classify the post-Chernobyl series (14 sporadic and 12 post-Chernobyl PTC), and reciprocally of gene subsets of the post-Chernobyl signature to classify the post radiotherapy series (15 sporadic and 12 post radiotherapy PTC), by using conventional principal component analysis. We found that the five genes common to the two signatures classified the learning/training tumors (used to search these signatures) of both the post radiotherapy (seven PTC) and the post-Chernobyl (six PTC) thyroid tumor series as compared with the sporadic tumors (seven sporadic PTC in each series). Importantly, these five genes were also effective for classifying independent series of post radiotherapy (five PTC) and post-Chernobyl (six PTC) tumors compared to independent series of sporadic tumors (eight PTC and six PTC respectively; testing tumors). Moreover, part of each post radiotherapy (32 genes) and post-Chernobyl signature (16 genes) cross-classified the respective series of thyroid tumors. Finally, several molecular pathways deregulated in post

  19. A Theory’s Travelogue: Post-Colonial Theory in Post-Socialist Space

    Czech Academy of Sciences Publication Activity Database

    Hladík, Radim

    2011-01-01

    Roč. 33, č. 4 (2011), s. 561-590 ISSN 1210-0250 R&D Projects: GA ČR(CZ) GAP401/11/2338 Institutional research plan: CEZ:AV0Z90090514 Keywords : post-socialism * post-colonialism * traveling theory * comparative perspective * post-communism Subject RIV: AA - Philosophy ; Religion

  20. Identification and analytical characterization of nine synthetic cathinone derivatives N-ethylhexedrone, 4-Cl-pentedrone, 4-Cl-α-EAPP, propylone, N-ethylnorpentylone, 6-MeO-bk-MDMA, α-PiHP, 4-Cl-α-PHP, and 4-F-α-PHP.

    Science.gov (United States)

    Liu, Cuimei; Jia, Wei; Li, Tao; Hua, Zhendong; Qian, Zhenhua

    2017-08-01

    Clinical and forensic toxicology laboratories are continuously confronted by analytical challenges when dealing with the new psychoactive substances (NPS) phenomenon. In this study, the analytical characterization of nine synthetic cathinones is described: 2-(ethylamino)-1-phenylhexan-1-one (N-ethylhexedrone 1), 1-(4-chlorophenyl)-2-(methylamino)pentan-1-one (4-Cl-pentedrone 2), 1-(4-chlorophenyl)-2-(ethylamino)pentan-1-one (4-Cl-α-EAPP 3), 1-(3,4-methylenedioxyphenyl)-2-propylaminopropan-1-one (propylone 4), 1-(3,4-methylenedioxyphenyl)-2-ethylaminopentan-1-one (N-ethylnorpentylone 5), 1-(6-methoxy-3,4-methylenedioxyphenyl)-2-methylaminopropan-1-one (6-MeO-bk-MDMA 6), 4-methyl-1-phenyl-2-(pyrrolidin-1-yl)pentan-1-one (α-PiHP 7), 1-(4-chlorophenyl)-2-(pyrrolidin-1-yl)hexan-1-one (4-Cl-α-PHP 8), and 1-(4-fluorophenyl)-2-(pyrrolidin-1-yl)hexan-1-one (4-F-α-PHP 9). The identification was based on ultra-high-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (UHPLC-QTOF-MS), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy. The mass-spectral fragmentations of these compounds following collision-induced dissociation (CID) and electron ionization (EI) were studied to assist forensic laboratories in identifying these compounds or other substances with similar structure in their case work. To our knowledge, no analytical data about the compounds 1-4, 7, and 8 have appeared until now, making this the first report on these compounds. The GC-MS data of 5, 6 and 9 has been reported, but this study added the LC-MS, Fourier Transform Infrared (FTIR) and NMR data for additional characterization. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  1. Post-traumatic Stress Disorder

    Directory of Open Access Journals (Sweden)

    H Javidi

    2011-12-01

    Full Text Available Unexpected extreme sudden traumatic stressor may cause post-traumatic stress disorder (PTSD. Important traumatic events include war, violent personal assault (e.g., sexual assault, and physical attack, being taken hostage or kidnapped, confinement as a prisoner of war, torture, terrorist attack, severe car accidents, and natural disasters. In childhood age sexual abuse or witnessing serious injuries or unexpected death of a beloved one are among important traumatic events. PTSD can be categorized into two types of acute and chronic PTSD: if symptoms persist for less than three months, it is termed “acute PTSD,” otherwise, it is called “chronic PTSD.” 60.7% of men and 51.2% of women would experience at least one potentially traumatic event in their lifetime. The lifetime prevalence of PTSD is significantly higher in women than men. Lifetime prevalence of PTSD varies from 0.3% in China to 6.1% in New Zealand. The prevalence of PTSD in crime victims are between 19% and 75%; rates as high as 80% have been reported following rape. The prevalence of PTSD among direct victims of disasters was reported to be 30%–40%; the rate in rescue workers was 10%–20%. The prevalence of PTSD among police, fire, and emergency service workers ranged from 6%–32%. An overall prevalence rate of 4% for the general population, the rate in rescue/recovery occupations ranged from 5% to 32%, with the highest rate reported in search and rescue personnel (25%, firefighters (21%, and workers with no prior training for facing disaster. War is one of the most intense stressors known to man. Armed forces have a higher prevalence of depression, anxiety disorders, alcohol abuse and PTSD. High-risk children who have been abused or experienced natural disasters may have an even higher prevalence of PTSD than adults. Female gender, previous psychiatric problem, intensity and nature of exposure to the traumatic event, and lack of social support are known risk factors for

  2. Post-caesarean analgesia: What is new?

    Directory of Open Access Journals (Sweden)

    Sukhyanti Kerai

    2017-01-01

    Full Text Available Adequate post-operative analgesia after caesarean section (CS is vital as it impacts the distinct surgical recovery requirements of the parturient. Although newer analgesic modalities and drugs for post-caesarean analgesia have been introduced over the recent years, review of the literature suggests suggests that we are far from achieving the goals of optimum post-operative analgesia. We conducted a systematic review of recent advances in modalities for post-caesarean analgesia. After systematic search and quality assessment of studies, we included a total of 51 randomised controlled trials that evaluated the role of opioids, transversus abdominis plane (TAP block, wound infiltration/infusion, ketamine, gabapentin and ilioinguinal-iliohypogastric nerve block (II-IH NB for post-caesarean analgesia. Administration of opioids still remains the gold standard for post-operative analgesia, but the associated troublesome side effects have led to the mandatory incorporation of non-opioid analgesics in post-CS analgesia regime. Among the non-opioid techniques, TAP block is the most investigated modality of the last decade. The analgesic efficacy of TAP block as a part of multimodal analgesia is established in post-CS cases where intrathecal morphine is not employed and in CS under general anaesthesia. Among non-steroidal anti-inflammatory drugs, COX-I inhibitors and intravenous paracetamol are found to be useful in post-operative analgesic regimen. The perioperative use of ketamine is found useful only in CS done under spinal anaesthesia; no benefit is seen where general anaesthesia is employed. Wound infiltration with local anaesthetics, systemic gabapentin and II-IH NB need further trials to assess their efficacy.

  3. Post-myelogram headache - physiological or psychological?

    International Nuclear Information System (INIS)

    Lee, T.; Maynard, N.; Briggs, M.; Anslow, P.; McPherson, K.; Northover, J.

    1991-01-01

    Psychological aspects of post-lumbar puncture headache have hitherto received little attention. A hundred consecutive patients admitted for elective myelography were studied. Post-myelogram headache was assessed by an independent observer and found to be strongly associated with normality of the test (P<0.001). Psychological testing showed a trend between Hospital Anxiety Depression score and normality of myelogram as well as development of headache, although this did not achieve statistical significance. This study suggestet that there is a large psychological as well as organic component to post-myelogram headache. (orig.)

  4. Vistas of the post-industrial city.

    Directory of Open Access Journals (Sweden)

    Malcolm Miles

    2000-02-01

    prosperity, as redundant industrial buildings are re-coded as sites of culture. But if the post-industrial city is a post-modern site of abundance, its benefits are unevenly distributed; centres of affluence construct margins of deprivation. Just as public art lent a veneer of cultural value to urban development in the 1980s, so the re-coding of industrial buildings as sites of culture in the ’90s contributes to a continuing aestheticisation of the city which affirms a dominant spatial order. What strategies, then, are appropriate for art in post-industrial cities?

  5. Post-epilepsy stroke: A review.

    Science.gov (United States)

    Jin, Jing; Chen, Rong; Xiao, Zheng

    2016-01-01

    Stroke and epilepsy are two of the most common neurological disorders and share a complicated relationship. It is well established that stroke is one of the most important causes of epilepsy, particularly new-onset epilepsy among the elderly. However, post-epilepsy stroke has been overlooked. In recent years, it has been demonstrated that epilepsy patients have increased risk and mortality from stroke when compared with the general population. Additionally, it was proposed that post-epilepsy stroke might be associated with antiepileptic drugs (AEDs), epileptic seizures and the lifestyle of epileptic patients. Here, we comprehensively review the epidemiology, causes and interventions for post-epilepsy stroke.

  6. Post-Traumatic Stress Disorder (PDQ)

    Science.gov (United States)

    ... stress (PTS) is a lot like post-traumatic stress disorder (PTSD) but not as severe. Patients have a ... PTS) are a lot like symptoms of other stress-related disorders. PTS has many of the same symptoms as ...

  7. Leukoencephalopaty post chemo and radiotherapy. CT Aspects

    Energy Technology Data Exchange (ETDEWEB)

    Potente, G; Iozzino, M; Amici, A; Vitturini, A; Capua, A; Tombolini, V

    1987-01-01

    22 patients submitted to radio chemotherapy of the central nervous system have been examined. 6 of them (28%) showed at Ct signs of post radiochemotherapic lekoencephalopathy 4 of them suffered of acute myeloid leukemia and 2 of acute lymphoblastic leukemia.

  8. 2011 Mayhill Post Fire Tile Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — This dataset serves as an index for the Mayhill Post Fire imagery for the Carson National Forest. The imagery was flown to provide coverage after the 2011 Pacheco...

  9. Russia's science minister keeps cabinet post

    CERN Multimedia

    1999-01-01

    Mikhail Kirpichnikov, minister for science and technology has kept his post in the cabinet of the new prime minister Sergei Stepashin. Yevgeniy Adamov also remains as minister of atomic energy (2 paragraphs).

  10. Social consciousness in post-conflict reconstruction.

    NARCIS (Netherlands)

    Gans, Ben; Rutkowski, Anne; Kaminski, B.; Kersten, G.E.; Szapiro, T.

    This paper sheds light on the complexities intergovernmental organizations are facing during post-conflict reconstruction. The article discusses the added-value of Social Responsibility in the context of the Comprehensive Approach, involving collaboration amongst defense, diplomacy and development.

  11. From Academic to Post-Academic Science

    Directory of Open Access Journals (Sweden)

    Mohammad Amin Ghaneirad

    2014-03-01

    Full Text Available This paper studies the cultural change in science from academic science to post-academic science by the use of documentary studying and analytical reasoning. The aim of this study is determining the direction of cultural change in science and comparing it with cultural change in society.The knowledge production which surrounds academy has little relationship with the values of society and epistemological norms regulate scientists' behavior from within the scientific system. But in post-academic science the relationship between science and society operates in the same line with market and government and science produce within the social context and scientists' behavior controlled by the norms out of the scientific system. So the culture of science has changed because science applied to meet the requirements of market and industry. The result is that contrary to cultural change in society that goes from materialism to post-materialism, cultural change in science moves from post-materialism to materialism.

  12. Philosophy and Post-Totalitarian Practices

    Directory of Open Access Journals (Sweden)

    Serhii Yosypenko

    2018-04-01

    Full Text Available This writing aims to outline the principles of researches on philosophy in Central and Eastern European countries, preferably USSR, in the latest soviet and post-soviet periods. In author’s opinion, the crucial points for such kind of research are: a to discover a correlation between philosophy and the phenomenon of totalitarianism; b to correlate a soviet philosophy with totalitarian experience. The article considers methodological and axiological problems in research of post-totalitarian practices in general as such as in philosophy. In author’s opinion the main problem in development of the post-soviet philosophy is interiorisation of intellectual, cultural and social practices, which were formed concerning to totalitarian experience. This became a reason of “cynicism” and “nihilism” of post-soviet philosophy. It’s impossible to cast mentioned phenomena off without consideration of totalitarian phenomenon and critical reconsideration of the own totalitarian experience.

  13. Knowledge, Attitude and Practices toward Post Exposure ...

    African Journals Online (AJOL)

    Knowledge, Attitude and Practices toward Post Exposure Prophylaxis for Human Immunodeficiency ... Annals of Medical and Health Sciences Research ... Data related to HIV PEP was collected by pre‑designed, pre‑tested, self‑administered ...

  14. 76 FR 57767 - Post Office Closing

    Science.gov (United States)

    2011-09-16

    ... determination to close the Ionia post office in Ionia, Missouri. The petition was filed by William Smart, Mayor... the interest of expedition, in light of the 120-day decision schedule, the Commission may request the...

  15. Swiss post office on the Meyrin site

    CERN Multimedia

    2006-01-01

    As of 4 September 2006, the post office on the Meyrin site will be open to the public from 9.00 a.m. to 4.00 p.m non-stop Mondays to Fridays. NB: the Meyrin 1 post office (23, avenue de Feuillasse) is open from 8.00 a.m. to 6.00 p.m. and the Meyrin 2 Village post office (3, chemin Antoine-Verchère) from 8.00 a.m. to 12.00 a.m. and from 3.00 p.m. to 6.00 p.m. For further information, please contact the Meyin Site post office on 72798. Relations with the Host States Service http://www.cern.ch/relations/ relations.secretariat@cern.ch Tel. 72848

  16. SWISS POST OFFICE ON THE MEYRIN SITE

    CERN Multimedia

    2006-01-01

    As of 4 September 2006, the post office on the Meyrin site will be open to the public from 9.00 a.m. to 4.00 p.m non-stop Mondays to Fridays. NB: the Meyrin 1 post office (23, avenue de Feuillasse) is open from 8.00 a.m. to 6.00 p.m. and the Meyrin 2 Village post office (3, chemin Antoine-Verchère) from 8.00 a.m. to 12.00 a.m. and from 3.00 p.m. to 6.00 p.m. For further information, please contact the Meyin Site post office on 72798. Relations with the Host States Service http://www.cern.ch/relations/ relations.secretariat@cern.ch Tel. 72848

  17. PRIMIPARA POST PARTUM DEPRESSION DIAGNOSIS AND TREATMENT

    Directory of Open Access Journals (Sweden)

    Esha Pradnyana

    2013-02-01

    Full Text Available Pregnancy and the first child birth is an influential complex event for a mother, which is where everything including phsycal and psyological aspects. This change can make mother psyological disorder, that can lead into depression after childbearing that call post childbearing depression or post partum depression. A wide review at 59 study make a result that 13% among primipara can suffer post partum depression 12 weeks after childbearing. Estabilishmet of this diagnosis, besides from history and symptoms, and can be supported through test Edinburgh Postnatal Depression Scale( EPDS . Patient with post partum depression, given treatment with antidepressant drug. Breastfeeding is not only to reduce stress for the mother, but also reduce the level of stress on a baby when his mother suffered depression

  18. The Kurds in Post-Saddam Iraq

    National Research Council Canada - National Science Library

    Katzman, Kenneth

    2008-01-01

    The Kurdish-inhabited region of northern Iraq is relatively peaceful and prospering economically, but the Iraqi Kurds political autonomy and political strength in post- Saddam Iraq is causing friction...

  19. Democracy in a Post-Castro Cuba?

    National Research Council Canada - National Science Library

    Henry, Drew

    2004-01-01

    .... The theories of leading democracy and economic theorists are applied to the post-Castro conflict scenario as relevant issues to be addressed by a new Cuban government and the United States in a Cuban...

  20. Post-communism: postmodernity or modernity revisited?

    Science.gov (United States)

    Ray, L

    1997-12-01

    Coinciding with the popularity of postmodern theory, the fall of communism appeared to offer further evidence of the exhaustion of modernity. Such analysis is grounded in a view that the Soviet system was the epitome of modernity. An alternative approach regards post-communism as opening new terrains of struggle for modernity. Thus Habermas and others suggest that post-communist societies are rejoining the trajectory of western modernity whose problems they now recapitulate. This alternative view implies that Soviet systems were something other than 'modern', although their nature is not always clearly defined. However, even if post-communist societies do encounter problems of modernity, they do so in new circumstances where modernist notions of social development have become problematic. This article argues that, contrary to those who regard modernization or postmodernization as irresistible trends, core post-communist societies are likely to develop along an alternative path to that of western modernity. This is tentatively described as 'neo-mercantilist'.

  1. A post-processor for Gurmukhi OCR

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    OCR output to perform post-processing (Church et al 1990). ... enhance text recognition in degraded text images such as multiple generation photocopy or facsimile. Not much .... In case of bi-gram analysis the prediction of the next character.

  2. Strengthening Democratic Governance through ICTs : Post Election ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Strengthening Democratic Governance through ICTs : Post Election ... has had several consequences, culminating in the disputed general elections of December 2007. ... Linking research to urban planning at the ICLEI World Congress 2018.

  3. Post-Retrieval Extinction Attenuates Cocaine Memories

    OpenAIRE

    Sartor, Gregory C; Aston-Jones, Gary

    2013-01-01

    Recent studies have shown that post-retrieval extinction training attenuates fear and reward-related memories in both humans and rodents. This noninvasive, behavioral approach has the potential to be used in clinical settings to treat maladaptive memories that underlie several psychiatric disorders, including drug addiction. However, few studies to date have used a post-retrieval extinction approach to attenuate addiction-related memories. In the current study, we attempted to disrupt cocaine...

  4. [Post-operative infections after cosmetic tourism].

    Science.gov (United States)

    Holst-Albrechtsen, Sine; Sørensen, Lene Birk; Juel, Jacob

    2018-06-11

    Cosmetic tourism is defined as patient mobility across borders, typically constituted by patients seeking cosmetic surgery at lower costs abroad. The most common procedures are abdominoplasty, fat grafting and breast augmentation. Very little is known about the complication rates after cosmetic tourism, and there is a paucity of evidence in all aspects of cosmetic tourism. In this review, we focus on post-operative complications i.e. post-operative infections, in particular with rare microorganisms such as mycobacteria.

  5. Cantilever steel post damaged by wind

    Directory of Open Access Journals (Sweden)

    Wei Sha

    2014-10-01

    Full Text Available An analysis for the cause of fracture failure of a cantilever steel sign post damaged by wind has been carried out. An unusual cause of failure has been identified, which is the subject of this paper. Microscopy and microanalysis of the fracture surface showed that the failure was due to pre-existing cracks, from the fabrication of the post. This conclusion was reached after detecting and analysing a galvanised layer on the fracture surfaces.

  6. Marijuana-Related Posts on Instagram

    OpenAIRE

    Cavazos-Rehg, Patricia A.; Krauss, Melissa J.; Sowles, Shaina J.; Bierut, Laura J.

    2016-01-01

    Instagram is a highly visual social networking site whose audience continues to grow, especially among young adults. In the present study, we examine marijuana-related content on Instagram to better understand the varied types of marijuana-related social networking occurring on this popular social media platform. We collected 417,561 Instagram posts with marijuana-related hashtags from November 29 to December 12, 2014. We assessed content of a random sample (n = 5000) of these posts with mari...

  7. Gender-Sensitive Post-Disaster Assessments

    OpenAIRE

    World Bank

    2012-01-01

    This note on gender-sensitive post-disaster assessments is the eighth in a series of guidance notes on gender issues in disaster risk management (DRM) in the East Asia and the Pacific region. Targeting World Bank staff, clients and development partners, this note gives an overview of the main reasons for assessing gender impacts as part of a post-disaster needs assessment, identifies the k...

  8. Content analysis of cancer blog posts.

    Science.gov (United States)

    Kim, Sujin

    2009-10-01

    The efficacy of user-defined subject tagging and software-generated subject tagging for describing and organizing cancer blog contents was explored. The Technorati search engine was used to search the blogosphere for cancer blog postings generated during a two-month period. Postings were mined for relevant subject concepts, and blogger-defined tags and Text Analysis Portal for Research (TAPoR) software-defined tags were generated for each message. Descriptive data were collected, and the blogger-defined tags were compared with software-generated tags. Three standard vocabularies (Opinion Templates, Basic Resource, and Medical Subject Headings [MeSH] Resource) were used to assign subject terms to the blogs, with results compared for efficacy in information retrieval. Descriptive data showed that most of the studied cancer blogs (80%) contained fewer than 500 words each. The numbers of blogger-defined tags per posting (M = 4.49 per posting) were significantly smaller than the TAPoR keywords (M = 23.55 per posting). Both blogger-defined subject tags and software-generated subject tags were often overly broad or overly narrow in focus, producing less than effective search results for those seeking to extract information from cancer blogs. Additional exploration into methods for systematically organizing cancer blog postings is necessary if blogs are to become stable and efficacious information resources for cancer patients, friends, families, or providers.

  9. Post-human body and beauty.

    Science.gov (United States)

    Russo, Maria Teresa; Di Stefano, Nicola

    2014-01-01

    The article calls into question the very possibility of a post-human aesthetics, starting from the following premise: rather than post-human, it is more correct to speak of post-natural, indicating by this expression a reality produced through a new type of evolution, which does not simply change human nature, but de-natures it, radically transforming it into an artefact. This post-nature which aspires to be perfect, immortal, invulnerable, is entirely devoid of beauty. In fact, while there may be an aesthetic of the artificial and of the artefact if it is in relation to objects, there is, however, no aesthetic of the post-human body. This is because is configured as a non-body and does not have the characteristics for what is commonly intended as beauty (harmony between matter and form, a reflection of inner life, uniqueness). Also in this case, it is more correct to speak of post-beauty, which in its properties appears to be the mirror image of beauty and ultimately, represents its complete dissolution.

  10. Post What? Disarticulating Post-Discourses in Toni Morrison’s God Help the Child

    Directory of Open Access Journals (Sweden)

    Delphine Gras

    2016-09-01

    Full Text Available In the midst of the proliferation of post-discourses, this essay investigates how Toni Morrison’s God Help the Child (2015 offers a timely exploration of the hurting Black female body that calls into question, if not outright refutes, whether Americans have entered a post-racial, post-Black, and post-feminist era. This essay opens with a critical context section that situates God Help the Child within and against post-discourses, before examining how resemblances with Morrison’s prior works like Beloved (1987 and The Bluest Eye (1970 confirm that the legacy of slavery still dictates the way Black female bodies are seen and treated in twenty-first-century America. Ultimately, what this study intends is to speak the unspeakable: race still matters despite the silencing effects of post-discourses.

  11. Influence of post pattern and resin cement curing mode on the retention of glass fibre posts.

    Science.gov (United States)

    Poskus, L T; Sgura, R; Paragó, F E M; Silva, E M; Guimarães, J G A

    2010-04-01

    To evaluate the influence of post design and roughness and cement system (dual- or self-cured) on the retention of glass fibre posts. Two tapered and smooth posts (Exacto Cônico No. 2 and White Post No. 1) and two parallel-sided and serrated posts (Fibrekor 1.25 mm and Reforpost No. 2) were adhesively luted with two different resin cements--a dual-cured (Rely-X ARC) and a self-cured (Cement Post)--in 40 single-rooted teeth. The teeth were divided into eight experimental groups (n = 5): PFD--Parallel-serrated-Fibrekor/dual-cured; PRD--Parallel-serrated-Reforpost/dual-cured; TED--Tapered-smooth-Exacto Cônico/dual-cured; TWD--Tapered-smooth-White Post/dual-cured; PFS--Parallel-serrated-Fibrekor/self-cured; PRS--Parallel-serrated-Reforpost/self-cured; TES--Tapered-smooth-Exacto Cônico/self-cured; TWS--Tapered-smooth-White Post/self-cured. The specimens were submitted to a pull-out test at a crosshead speed of 0.5 mm min(-1). Data were analysed using analysis of variance and Bonferroni's multiple comparison test (alpha = 0.05). Pull-out results (MPa) were: PFD = 8.13 (+/-1.71); PRD = 8.30 (+/-0.46); TED = 8.68 (+/-1.71); TWD = 9.35 (+/-1.99); PFS = 8.54 (+/-2.23); PRS = 7.09 (+/-1.96); TES = 8.27 (+/-3.92); TWS = 7.57 (+/-2.35). No statistical significant difference was detected for posts and cement factors and their interaction. The retention of glass fibre posts was not affected by post design or surface roughness nor by resin cement-curing mode. These results imply that the choice for serrated posts and self-cured cements is not related to an improvement in retention.

  12. the occurrence of post partum anoestrus in bonsmara cows

    African Journals Online (AJOL)

    duration of post partum anoestrus than gain in body mass. Post pactum ... and Bos Taurus cattle to improve fertility and milk pro- duction in high .... The occurrence of post partum anoestrus in beef cows under ranching conditions. Proc.

  13. Post-infectious sequelae of travelers' diarrhea.

    Science.gov (United States)

    Connor, Bradley A; Riddle, Mark S

    2013-01-01

    Travelers' diarrhea (TD) has generally been considered a self-limited disorder which resolves more quickly with expeditious and appropriate antibiotic therapy given bacteria are the most frequently identified cause. However, epidemiological, clinical, and basic science evidence identifying a number of chronic health conditions related to these infections has recently emerged which challenges this current paradigm. These include serious and potentially disabling enteric and extra-intestinal long-term complications. Among these are rheumatologic, neurologic, gastrointestinal, renal, and endocrine disorders. This review aims to examine and summarize the current literature pertaining to three of these post-infectious disorders: reactive arthritis, Guillain-Barré syndrome, and post-infectious irritable bowel syndrome and the relationship of these conditions to diarrhea associated with travel as well as to diarrhea associated with gastroenteritis which may not be specifically travel related but relevant by shared microbial pathogens. It is hoped this review will allow clinicians who see travelers to be aware of these post-infectious sequelae thus adding to our body of knowledge in travel medicine. Data for this article were identified by searches of PubMed and MEDLINE, and references from relevant articles using search terms "travelers' diarrhea" "reactive arthritis" "Guillain-Barré syndrome" "Post-Infectious Irritable Bowel Syndrome." Abstracts were included when related to previously published work. A review of the published literature reveals that potential consequences of travelers' diarrhea may extend beyond the acute illness and these post-infectious complications may be more common than currently recognized. In addition since TD is such a common occurrence it would be helpful to be able to identify those who might be at greater risk of post-infectious sequelae in order to target more aggressive prophylactic or therapeutic approaches to such individuals. It is

  14. Management of rigid post-traumatic kyphosis.

    Science.gov (United States)

    Wu, S S; Hwa, S Y; Lin, L C; Pai, W M; Chen, P Q; Au, M K

    1996-10-01

    Rigid post-traumatic kyphosis after fracture of the thoracolumbar and lumbar spine represents a failure of initial management of the injury. Kyphosis moves the center of gravity anterior. The kyphosis and instability may result in pain, deformity, and increased neurologic deficits. Management for symptomatic post-traumatic kyphosis always has presented a challenge to orthopedic surgeons. To evaluate the surgical results of one stage posterior correction for rigid symptomatic post-traumatic kyphosis of the thoracolumbar and lumbar spine. The management for post-traumatic kyphosis remains controversial. Anterior, posterior, or combined anterior and posterior procedures have been advocated by different authors and show various degrees of success. One vertebra immediately above and below the level of the deformity was instrumented posteriorly by a transpedicular system (internal fixator AO). Posterior decompression was performed by excision of the spinal process and bilateral laminectomy. With the deformed vertebra through the pedicle, the vertebral body carefully is removed around the pedicle level, approximating a wedge shape. The extent to which the deformed vertebral body should be removed is determined by the attempted correction. Correction of the deformity is achieved by manipulation of the operating table and compression of the adjacent Schanz screws above and below the lesion. Thirteen patients with post-traumatic kyphosis with symptoms of fatigue and pain caused by slow progression of kyphotic deformities received posterior decompression, correction, and stabilization as a definitive treatment. The precorrection kyphosis ranged from 30-60 degrees, with a mean of 40 degrees +/- 10.8 degrees. After correction, kyphosis was reduced to an average of 1.5 degrees +/- 3.8 degrees, with a range from -5 degrees to 5 degrees. The average angle of correction was 38.8 degrees +/- 10.4 degrees, with a range from 25 degrees to 60 degrees. Significant difference was found

  15. Marijuana-Related Posts on Instagram.

    Science.gov (United States)

    Cavazos-Rehg, Patricia A; Krauss, Melissa J; Sowles, Shaina J; Bierut, Laura J

    2016-08-01

    Instagram is a highly visual social networking site whose audience continues to grow, especially among young adults. In the present study, we examine marijuana-related content on Instagram to better understand the varied types of marijuana-related social networking occurring on this popular social media platform. We collected 417,561 Instagram posts with marijuana-related hashtags from November 29 to December 12, 2014. We assessed content of a random sample (n = 5000) of these posts with marijuana-related hashtags. Approximately 2136 (43 %) were explicit about marijuana and further analyzed. Of the 2136 marijuana-related posts, images of marijuana were common (n = 1568). Among these 1568 marijuana images, traditional forms (i.e., buds/leaves) were the most common (63 %), followed by some novel forms of marijuana, including marijuana concentrates (20 %). Among the 568 posts that displayed marijuana being ingested, 20 % showed someone dabbing marijuana concentrates. Marijuana-related advertisements were also observed among the 2136 marijuana-related posts (9 %). Our findings signal the promotion of marijuana use in its traditional plant-based form; trendy and novel modes of marijuana ingestion were also endorsed. This content along with the explicit marketing of marijuana that we observed on Instagram have potential to influence social norms surrounding marijuana use.

  16. Post-Colonial Theory and Action Research

    Directory of Open Access Journals (Sweden)

    Jim Parsons

    2011-02-01

    Full Text Available This essay explores connections between post-colonial theory and action research. Post-colonial theory is committed to addressing the plague of colonialism. Action research, at its core, promises to problematize uncontested ‘colonial’ hegemonies of any form. Both post-colonial theory and action research engage dialogic, critically reflective and collaborative values to offer a fuller range of human wisdom. The authors contend that post-colonialism theory calls for justice and seeks to speak to social and psychological suffering, exploitation, violence and enslavement done to the powerless victims of colonization around the world by challenging the superiority of dominant perspectives and seeking to re-position and empower the marginalized and subordinated. In similar ways, action research works to eradicate oppression, powerlessness and worthlessness by affirming solidarity with the oppressed, helping humans move from passive to active and by fundamentally reshaping power. Because both post-colonial theory and action research position the insider or oppressed in an ethic of efficacy, it values community, relationships, communication and equality, and is committed to reciprocity, reflexivity and reflection. Thus, both hold the potential to help reconstruct conditions for a more democratic and just society

  17. Post-partum acute kidney injury

    Directory of Open Access Journals (Sweden)

    Naresh Pahwa

    2014-01-01

    Full Text Available To determine the risk factors, course of hospital stay and mortality rate among women with post-partum acute kidney injury (AKI, we studied (of 752 patients with AKI admitted to a tertiary care center during the study period between November 2009 and August 2012 27 (3.59% women with post-partum AKI. The data regarding age, parity, cause of renal failure, course of hospital stay and requirement of dialysis were recorded. Sepsis was the major cause (70.3% of post-partum AKI. Other causes included disseminated intravascular coagulation (55.5%, pre-eclampsia/eclampsia (40.7%, ante- and post-partum hemorrhage (40.7% and 22.2% and hemolytic anemia and elevated liver enzymes and low platelet count syndrome (29.6%; most patients had more than one cause of AKI. We found a very high prevalence (18.5% of cortical necrosis in our study patients. A significant correlation was also found between the creatinine level on admission and the period of onset of disease after delivery. In conclusion, several factors are involved in causing post-partum AKI in our population, and sepsis was the most common of them.

  18. Marijuana-Related Posts on Instagram

    Science.gov (United States)

    Krauss, Melissa J.; Sowles, Shaina J.; Bierut, Laura J.

    2016-01-01

    Instagram is a highly visual social networking site whose audience continues to grow, especially among young adults. In the present study, we examine marijuana-related content on Instagram to better understand the varied types of marijuana-related social networking occurring on this popular social media platform. We collected 417,561 Instagram posts with marijuana-related hashtags from November 29 to December 12, 2014. We assessed content of a random sample (n = 5000) of these posts with marijuana-related hashtags. Approximately 2136 (43 %) were explicit about marijuana and further analyzed. Of the 2136 marijuana-related posts, images of marijuana were common (n = 1568). Among these 1568 marijuana images, traditional forms (i.e., buds/leaves) were the most common (63 %), followed by some novel forms of marijuana, including marijuana concentrates (20 %). Among the 568 posts that displayed marijuana being ingested, 20 % showed someone dabbing marijuana concentrates. Marijuana-related advertisements were also observed among the 2136 marijuana-related posts (9 %). Our findings signal the promotion of marijuana use in its traditional plant-based form; trendy and novel modes of marijuana ingestion were also endorsed. This content along with the explicit marketing of marijuana that we observed on Instagram have potential to influence social norms surrounding marijuana use. PMID:27262456

  19. Post-Colonial Theory and Action Research

    Directory of Open Access Journals (Sweden)

    Jim B. Parsons

    2011-04-01

    Full Text Available This essay explores connections between post-colonial theory and action research. Post-colonial theory is committed to addressing the plague of colonialism. Action research, at its core, promises to problematize uncontested ‘colonial’ hegemonies of any form. Both post-colonial theory and action research engage dialogic, critically reflective and collaborative values to offer a fuller range of human wisdom. The authors contend that post-colonialism theory calls for justice and seeks to speak to social and psychological suffering, exploitation, violence and enslavement done to the powerless victims of colonization around the world by challenging the superiority of dominant perspectives and seeking to re-position and empower the marginalized and subordinated. In similar ways, action research works to eradicate oppression, powerlessness and worthlessness by affirming solidarity with the oppressed, helping humans move from passive to active and by fundamentally reshaping power. Because both post-colonial theory and action research position the insider or oppressed in an ethic of efficacy, it values community, relationships, communication and equality, and is committed to reciprocity, reflexivity and reflection. Thus, both hold the potential to help reconstruct conditions for a more democratic and just society.

  20. Post-event processing in social anxiety.

    Science.gov (United States)

    Dannahy, Laura; Stopa, Lusia

    2007-06-01

    Clark and Wells' [1995. A cognitive model of social phobia. In: R. Heimberg, M. Liebowitz, D.A. Hope, & F.R. Schneier (Eds.) Social phobia: Diagnosis, assessment and treatment (pp. 69-93). New York: Guildford Press.] cognitive model of social phobia proposes that following a social event, individuals with social phobia will engage in post-event processing, during which they conduct a detailed review of the event. This study investigated the relationship between self-appraisals of performance and post-event processing in individuals high and low in social anxiety. Participants appraised their performance immediately after a conversation with an unknown individual and prior to an anticipated second conversation task 1 week later. The frequency and valence of post-event processing during the week following the conversation was also assessed. The study also explored differences in the metacognitive processes of high and low socially anxious participants. The high socially anxious group experienced more anxiety, predicted worse performance, underestimated their actual performance, and engaged in more post-event processing than low socially anxious participants. The degree of negative post-event processing was linked to the extent of social anxiety and negative appraisals of performance, both immediately after the conversation task and 1 week later. Differences were also observed in some metacognitive processes. The results are discussed in relation to current theory and previous research.