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Sample records for bitis gabonica rhinoceros

  1. Purification and functional characterisation of rhiminopeptidase A, a novel aminopeptidase from the venom of Bitis gabonica rhinoceros.

    Directory of Open Access Journals (Sweden)

    Sakthivel Vaiyapuri

    2010-08-01

    Full Text Available Snake bite is a major neglected public health issue within poor communities living in the rural areas of several countries throughout the world. An estimated 2.5 million people are bitten by snakes each year and the cost and lack of efficacy of current anti-venom therapy, together with the lack of detailed knowledge about toxic components of venom and their modes of action, and the unavailability of treatments in rural areas mean that annually there are around 125,000 deaths worldwide. In order to develop cheaper and more effective therapeutics, the toxic components of snake venom and their modes of action need to be clearly understood. One particularly poorly understood component of snake venom is aminopeptidases. These are exo-metalloproteases, which, in mammals, are involved in important physiological functions such as the maintenance of blood pressure and brain function. Although aminopeptidase activities have been reported in some snake venoms, no detailed analysis of any individual snake venom aminopeptidases has been performed so far. As is the case for mammals, snake venom aminopeptidases may also play important roles in altering the physiological functions of victims during envenomation. In order to further understand this important group of snake venom enzymes we have isolated, functionally characterised and analysed the sequence-structure relationships of an aminopeptidase from the venom of the large, highly venomous West African gaboon viper, Bitis gabonica rhinoceros.The venom of B. g. rhinoceros was fractionated by size exclusion chromatography and fractions with aminopeptidase activities were isolated. Fractions with aminopeptidase activities showed a pure protein with a molecular weight of 150 kDa on SDS-PAGE. In the absence of calcium, this purified protein had broad aminopeptidase activities against acidic, basic and neutral amino acids but in the presence of calcium, it had only acidic aminopeptidase activity (APA. Together

  2. Movements and home range of the gaboon adder, Bitis gabonica ...

    African Journals Online (AJOL)

    Gaboon adders are active throughout the year, but are largely sedentary. In their prime habitat of forest–thicket–grassland mosaic in Zululand their normal foraging movements are short range, punctuated by long periods of inactivity. Occasional long distance movements suggest changes in foraging area, but these ...

  3. puff-adder, Bitis arietans

    African Journals Online (AJOL)

    1-76. The histology of the venom- secreting apparatus of the puff-adder, Bitis arietans. A.R. Lake, J. Hattingh·, R.E. King and T.R. Trevor-Jones. Departments of General Anatomy and General Physiology,. Dental School, University of the Witwatersrand, Johannesburg 2001. -To whom correspondence should be addressed.

  4. (Bitis; Viperidae) from the Western Cape Province, South Africa

    African Journals Online (AJOL)

    1997-02-25

    Feb 25, 1997 ... Dwarf adders of the genus Bitis are popular in the interna- tional pet trade in reptiles, and although ... dwarf adder, B. schneideri) are already threatened, in pan, by illegal collecting for this trade (Branch .... between the eyes at the anterior edge of the orbit; I I right, 12 left circumorbitals; two scale rows ...

  5. Rhinoceros sondaicus (Perissodactyla: Rhinocerotidae)

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    Leslie, David M.; Groves, Colin P.

    2011-01-01

    Rhinoceros sondaicus Desmarest, 1822, commonly called the Javan rhinoceros or lesser one-horned rhinoceros, is the most critically endangered large mammal on Earth with only 40-50 extant individuals in 2 disjunct and distant populations: most in Ujung Kulon, West Java, and only 2-6 (optimistically) in Cat Loc, Vietnam. R. sondaicus is polytypic with 3 recognized subspecies: R. s. sondaicus (currently West Java), R. s. inermis (formerly Sunderbunds; no doubt extinct), and R s. annamiticus (Vietnam; perhaps now extinct). R. sondaicus is a browser and currently occupies lowland semievergreen secondary forests in Ja va and marginal habitat in Vietnam; it was once more widespread and abundant, likely using a greater variety of habitats. R sondaicus has a very spotty history of husbandry, and no individuals are currently in captivity. Conservation focuses on protection from poaching and habitat loss. Following decades-long discussion of captive breeding and establishment of a 3rd wild population, conservation and governmental agencies appear closer to taking such seriously needed action on the latter.

  6. New proline-rich oligopeptides from the venom of African adders: Insights into the hypotensive effect of the venoms.

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    Kodama, Roberto T; Cajado-Carvalho, Daniela; Kuniyoshi, Alexandre K; Kitano, Eduardo S; Tashima, Alexandre K; Barna, Barbara F; Takakura, Ana Carolina; Serrano, Solange M T; Dias-Da-Silva, Wilmar; Tambourgi, Denise V; Portaro, Fernanda V

    2015-06-01

    The snakes from the Bitis genus are some of the most medically important venomous snakes in sub Saharan Africa, however little is known about the composition and effects of these snake venom peptides. Considering that the victims with Bitis genus snakes have exacerbate hypotension and cardiovascular disorders, we investigated here the presence of angiotensin-converting enzyme modulators on four different species of venoms. The peptide fractions from Bitis gabonica gabonica, Bitis nasicornis, Bitis gabonica rhinoceros and Bitis arietans which showed inhibitory activity on angiotensin-converting enzyme were subjected to mass spectrometry analysis. Eight proline-rich peptides were synthetized and their potencies were evaluated in vitro and in vivo. The MS analysis resulted in over 150 sequences, out of which 32 are new proline-rich oligopeptides, and eight were selected for syntheses. For some peptides, inhibition assays showed inhibitory potentials of cleavage of angiotensin I ten times greater when compared to bradykinin. In vivo tests showed that all peptides decreased mean arterial pressure, followed by tachycardia in 6 out of 8 of the tests. We describe here some new and already known proline-rich peptides, also known as bradykinin-potentiating peptides. Four synthetic peptides indicated a preferential inhibition of angiotensin-converting enzyme C-domain. In vivo studies show that the proline-rich oligopeptides are hypotensive molecules. Although proline-rich oligopeptides are known molecules, we present here 32 new sequences that are inhibitors of the angiotensin-converting enzyme and consistent with the symptoms of the victims of Bitis spp, who display severe hypotension. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Histology of the venom gland of the puff-adder (Bitis arietans)

    African Journals Online (AJOL)

    J. Morph. 123: 133-156. RUSSEL, F.E. & EVENTOV, R. 1964. Lethality of crude and lyopbilised Crotalus venom. Toxicon 2: 81. WILLEMSE, G.T., HATTINGH, J., KARLSSON, R.M., LEVY, S. &. PARKER, C. 1979. Changes in composition and protein concentration of puff-adder (Bitis arietans) venom due to frequent milking.

  8. Histological study of white rhinoceros integument

    OpenAIRE

    Plochocki, Jeffrey H.; Ruiz, Saul; Rodriguez-Sosa, Jos? R.; Hall, Margaret I.

    2017-01-01

    In this study, we report findings from a microscopic analysis of the white rhinoceros (Ceratotherium simum) integumentary ultrastructure. Skin samples from the cheek, shoulder, flank and rump were taken from a 46-year-old female southern white rhinoceros and examined using H&E and elastic histological stains. The epidermis was thickest in the flank (1.003 mm) followed by the rump, cheek and shoulder. The stratum corneum comprised more than half the epidermal thickness. Numerous melanin granul...

  9. Parathyroid, Thyroid and Recurrent Laryngeal Nerve Anatomy in an Indian Rhinoceros (Rhinoceros unicornis).

    Science.gov (United States)

    Udelsman, R; Citino, S B; Prasad, M; Donovan, P I; Fredholm, D V

    2018-02-01

    The parathyroid gland was first identified in the Indian rhinoceros in 1849 by Sir Richard Owen. We performed a necropsy in an Indian rhinoceros, recapitulating Owen's dissection and display what appear to be the initial identification of the recurrent laryngeal nerve in situ and the anatomy and histology of the largest rhinoceros parathyroid glands yet identified. Patrick T. Rhino, a 41-year-old Indian rhinoceros was born in 1974. His early years were unremarkable. In 2006, he was donated to White Oak Conservation in Yulee, Florida, where he bred and sustained minor injuries. In his geriatric years, he developed a cataract and degenerative joint disease (DJD). At age 41, he developed progressive ataxia and lameness and was euthanized to minimize suffering when he was unable to stand. ROS, FH, SH and medication history were unremarkable. Physical exam was age and species appropriate. Pre-mortem serum demonstrated: creat 1.8 mg/dL (0.8-2.1), calcium 10.6 mg/dL (9.7-13.1), phos 3.8 mg/dL (2.5-6.7), alk phos 69 U/L (26-158) and intact PTH 44.1 pg/mL (rhinoceros reference range: unknown). Necropsy revealed intervertebral DJD with thoracic spondylosis, which combined with osteoporosis, resulted in thoracic myelopathy and ataxia. The neck block was sent in formalin to the Yale University School of Medicine. Detailed dissection was performed under loupe magnification. Presumed structures were photographed in situ and biopsied. The thyroid was identified deep to the strap muscles, received its blood supply from the inferior and superior thyroid arteries and was blue in color. The right recurrent laryngeal nerve, identified and photographed in situ for the first time in the rhinoceros, was deep to the inferior thyroid artery and was traced throughout its cervical course. Single parathyroid glands identified on the lateral thyroid lobes received their blood supply from the inferior thyroid arteries and were confirmed histologically. They appear to be the largest

  10. Species identification of rhinoceros horns using the cytochrome b gene.

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    Hsieh, Hsing-Mei; Huang, Li-Hung; Tsai, Li-Chin; Kuo, Yi-Chen; Meng, Hsien-Huei; Linacre, Adrian; Lee, James Chun-I

    2003-09-09

    Material suspected of originating from species of Rhinoceros is frequently seized by forensic organizations investigating trade in endangered species. At present identification of the species is possible by DNA sequencing of the material, such as powdered rhinoceros horns. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. The maximum value of genetic distance among white rhinoceros was 0.0176, and 0.0333 among black rhinoceros. In the comparison among rhinoceros species, the greatest genetic distance was between black and Indian rhinoceros (0.1564). The rhinoceros sequences extracted from GenBank and 13 samples in this study were clustered and separated from other mammals. Holstein cow was used as an out-group and was clustered with cattle in the phylogenetic tree. The results of this phylogenetic study also showed that there were four major branches among rhinoceros species from a common origin. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. In the initial identification of species from unknown powdered material, all the unknown samples were found to be from rhinoceroses. In phylogenetic analysis, the results supported the morphological hypothesis. The method used in this study can be applied in the identification of processed products of rhinoceros horns, such as sculptures, daggers, powders or even mixture powdered prescriptions.

  11. Systemic neosporosis in a white rhinoceros.

    Science.gov (United States)

    Sommanustweechai, Angkana; Vongpakorn, Montakan; Kasantikul, Tanit; Taewnean, Jedsada; Siriaroonrat, Boripat; Bush, Mitchell; Pirarat, Nopadon

    2010-03-01

    Neosporosis was diagnosed in a 16-year-old female white rhinoceros (Ceratotherium simum) that died suddenly without clinical signs. Histopathology revealed disseminated protozoan tachyzoites in liver, adrenal cortex, kidney, and intestine, with morphology compatible with either Toxoplasma or Neospora. The organism was identified as Neospora caninum with the use of primary rabbit anti-N. caninum antibody immunohistochemistry and polymerase chain reaction. The exact source of infection remains unknown, but it is suspected that N. caninum oocysts were ingested from the soil.

  12. Histological study of white rhinoceros integument.

    Directory of Open Access Journals (Sweden)

    Jeffrey H Plochocki

    Full Text Available In this study, we report findings from a microscopic analysis of the white rhinoceros (Ceratotherium simum integumentary ultrastructure. Skin samples from the cheek, shoulder, flank and rump were taken from a 46-year-old female southern white rhinoceros and examined using H&E and elastic histological stains. The epidermis was thickest in the flank (1.003 mm followed by the rump, cheek and shoulder. The stratum corneum comprised more than half the epidermal thickness. Numerous melanin granules were found in the basal and spinosum layers. The epidermal-dermal junction was characterized by abundant papillary folds increasing surface contact between integument layers. Most of the dermal thickness consisted of organized collagen bundles with scattered elastic fibers. Collagen fiber bundles were thickest in the flank (210.9 μm followed by shoulder, rump and cheek. Simple coiled sweat glands were present in the dermis, but hair and sebaceous glands were absent. Together, these data suggest the white rhinoceros has a unique integumentary system among large terrestrial herbivores.

  13. Histological study of white rhinoceros integument.

    Science.gov (United States)

    Plochocki, Jeffrey H; Ruiz, Saul; Rodriguez-Sosa, José R; Hall, Margaret I

    2017-01-01

    In this study, we report findings from a microscopic analysis of the white rhinoceros (Ceratotherium simum) integumentary ultrastructure. Skin samples from the cheek, shoulder, flank and rump were taken from a 46-year-old female southern white rhinoceros and examined using H&E and elastic histological stains. The epidermis was thickest in the flank (1.003 mm) followed by the rump, cheek and shoulder. The stratum corneum comprised more than half the epidermal thickness. Numerous melanin granules were found in the basal and spinosum layers. The epidermal-dermal junction was characterized by abundant papillary folds increasing surface contact between integument layers. Most of the dermal thickness consisted of organized collagen bundles with scattered elastic fibers. Collagen fiber bundles were thickest in the flank (210.9 μm) followed by shoulder, rump and cheek. Simple coiled sweat glands were present in the dermis, but hair and sebaceous glands were absent. Together, these data suggest the white rhinoceros has a unique integumentary system among large terrestrial herbivores.

  14. In Vitro Toxic Effects of Puff Adder (Bitis arietans Venom, and Their Neutralization by Antivenom

    Directory of Open Access Journals (Sweden)

    Steven Fernandez

    2014-05-01

    Full Text Available This study investigated the in vitro toxic effects of Bitis arietans venom and the ability of antivenom produced by the South African Institute of Medical Research (SAIMR to neutralize these effects. The venom (50 µg/mL reduced nerve-mediated twitches of the chick biventer muscle to 19% ± 2% of initial magnitude (n = 4 within 2 h. This inhibitory effect of the venom was significantly attenuated by prior incubation of tissues with SAIMR antivenom (0.864 µg/µL; 67% ± 4%; P < 0.05; n = 3–5, unpaired t-test. Addition of antivenom at t50 failed to prevent further inhibition or reverse the inhibition of twitches and responses to agonists. The myotoxic action of the venom (50 µg/mL was evidenced by a decrease in direct twitches (30% ± 6% of the initial twitch magnitude and increase in baseline tension (by 0.7 ± 0.3 g within 3 h of the chick biventer. Antivenom failed to block these effects. Antivenom however prevented the venom induced cytotoxic effects on L6 skeletal muscle cells. Venom induced a marginal but significant reduction in plasma clotting times at concentrations above 7.8 µg/100 µL of plasma, indicating poor procoagulant effects. In addition, the results of western immunoblotting indicate strong immunoreactivity with venom proteins, thus warranting further detailed studies on the neutralization of the effects of individual venom toxins by antivenom.

  15. Pheochromocytoma in a white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Bertelsen, Mads F; Steele, Shelby L; Grøndahl, Carsten; Baandrup, Ulrik

    2011-09-01

    A 46-yr-old male white rhinoceros (Ceratotherium simum) died during anesthesia following agonal excitation. On postmortem, a well-demarcated 2.5-cm tan mass was identified in the right adrenal gland. Histopathology confirmed the presence of a pheochromocytoma, and elevated levels of epinephrine in serum collected shortly prior to the animal's death, as compared with sera from healthy controls, demonstrated the functional nature of the tumor. Although rare, pheochromocytoma should be considered as a differential diagnosis in cases of suspected hypertension and acute death in rhinos.

  16. Population densities of eastern black rhinoceros : unravelling the controls

    NARCIS (Netherlands)

    Ouma, B.O.

    2014-01-01

    Key words: black rhinoceros, browser, corticosterone, diet, density dependence, minerals, moisture, physiological stress, savanna, soil nutrients, woody cover.   Understanding the forces that cause variability in population sizes is a central theme in ecology. The limiting factor in

  17. Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya.

    Science.gov (United States)

    Otiende, Moses Y; Kivata, Mary W; Makumi, Joseph N; Mutinda, Mathew N; Okun, Daniel; Kariuki, Linus; Obanda, Vincent; Gakuya, Francis; Mijele, Dominic; Soriguer, Ramón C; Alasaad, Samer

    2015-01-17

    A huge effort in rhinoceros conservation has focused on poaching and habitat loss as factors leading to the dramatic declines in the endangered eastern black rhinoceros (Diceros bicornis michaeli) and the southern white rhinoceros (Ceratotherium simum simum). Nevertheless, the role disease and parasite infections play in the mortality of protected populations has largely received limited attention. Infections with piroplasmosis caused by Babesia bicornis and Theileria bicornis has been shown to be fatal especially in small and isolated populations in Tanzania and South Africa. However, the occurrence and epidemiology of these parasites in Kenyan rhinoceros is not known. Utilizing 18S rRNA gene as genetic marker to detect rhinoceros infection with Babesia and Theileria, we examined blood samples collected from seven rhinoceros populations consisting of 114 individuals of black and white rhinoceros. The goal was to determine the prevalence in Kenyan populations, and to assess the association of Babesia and Theileria infection with host species, age, sex, location, season and population mix (only black rhinoceros comparing to black and white rhinoceros populations). We did not detect any infection with Babesia in the sequenced samples, while the prevalence of T. bicornis in the Kenyan rhinoceros population was 49.12% (56/114). White rhinoceros had significantly higher prevalence of infection (66%) compared to black rhinoceros (43%). The infection of rhinoceros with Theileria was not associated with animal age, sex or location. The risk of infection with Theileria was not higher in mixed species populations compared to populations of pure black rhinoceros. In the rhinoceros studied, we did not detect the presence of Babesia bicornis, while Theileria bicornis was found to have a 49.12% prevalence with white rhinoceros showing a higher prevalence (66%) comparing with black rhinoceros (43%). Other factors such as age, sex, location, and population mix were not found to

  18. A bibliography of black rhinoceros Diceros bicomis (Linnaeus, 1758 and white rhinoceros Ceratotherium simum (Burchell, 1817 for southern Africa

    Directory of Open Access Journals (Sweden)

    Heather Wildi

    1989-10-01

    Full Text Available A general bibliography of reference material relevant to wildlife managers and researchers involved in the conservation of black and white rhinoceros in southern Africa is provided. It includes both key word and full reference listings.

  19. Estrus induction in white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Hermes, R; Hildebrandt, T B; Walzer, C; Göritz, F; Gray, C; Niemuller, C; Schwarzenberger, F

    2012-10-01

    The estrous cycle length in the white rhinoceros (Ceratotherium simum) is either 4 or 10 wk. The cause(s) for this variation as well as the poor fertility rate in captivity remains under debate in this species. Most captive adult white rhinoceros undergo long anovulatory periods without luteal activity which are considered a major reason for their low reproductive rate. In this study, the synthetic progestin chlormadinone acetate (CMA) was tested in combination with hCG or the GnRH analogue deslorelin for its efficiency to induce ovulation in fourteen females without luteal activity and in three, regular cycling females. HCG (N = 12), injectable GnRH analogue (N = 8) and GnRH analogue implants (N = 15) were given to induce ovulation after CMA treatment. Treatment success was determined using both transrectal ultrasonography and progesterone metabolite EIA analysis. A preovulatory sized follicle (3.5 ± 0.1 cm) or a corpus luteum (5.1 ± 0.7) was present on the ovary one day after induction in 93.1% of the treatments. Despite this high rate of ovarian response, ovulation rate differed between the study groups. The ovulation rate for hCG, injectable GnRH analogue and GnRH analogue implants was 66.7%, 62.5% and 93.3%, respectively. Ovulation rate in cyclic females treated with GnRH implants was 100% (6/6) compared with 89% (8/9) in females without luteal activity receiving the same treatment. The length of the estrous cycle when induced with hCG was 4 wk (85.7%). The estrous cycle when induced with GnRH analogue was predominantly 10 wk long. Two females without luteal activity treated with GnRH became pregnant. In conclusion, CMA in combination with GnRH analogue implants was highly effective to induce ovulation in white rhinoceroses and thus can contribute to efforts aimed at increasing natural mating and reproductive rates in the captive white rhinoceros population. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. Iron overload syndrome in the black rhinoceros (Diceros bicornis): microscopical lesions and comparison with other rhinoceros species.

    Science.gov (United States)

    Olias, P; Mundhenk, L; Bothe, M; Ochs, A; Gruber, A D; Klopfleisch, R

    2012-11-01

    The African black rhinoceros (Diceros bicornis) has adapted to a low iron diet during evolution and is thus prone to iron overload in captivity, which is associated with a number of serious disorders. A S88T polymorphism in the HFE gene has been suggested as a potential genetic basis of increased iron uptake in the black rhinoceros, while the Indian rhinoceros is thought to be unaffected by iron overload in captivity. In the present study, the histopathology and distribution of iron accumulations in five black rhinoceroses with iron overload syndrome were characterized and compared with three Indian rhinoceroses (Rhinoceros unicornis) and one African white rhinoceros (Ceratotherium simum). At necropsy examination, iron storage in black rhinoceroses was not associated with gross lesions. Microscopically, the most consistent and highest degree of iron load was found in the spleen, liver, small intestine and lung. There was minimal fibrosis and single cell necrosis in the liver. Endocrine organs, lymph nodes, heart and kidney were less often and less markedly affected. Unexpectedly, Indian rhinoceroses also showed iron load in the spleen and smaller amounts in organs similar to the black rhinoceros except for in the heart, while the white rhinoceros had only minor detectable iron storage in intestine, liver and lung. Sequence analysis confirmed the HFE S88T polymorphism in black but not in Indian rhinoceroses. The results indicate that Indian rhinoceroses may also be affected by iron storage in captivity, although in a milder form than the black rhinoceros, and therefore challenge the relevance of the S88T polymorphism in the HFE gene of black rhinoceroses as the underlying cause for iron overload. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. The seminiferous cycle of the rhinoceros.

    Science.gov (United States)

    Portas, Timothy; Evans, Gareth; de Graaf, Simon

    2012-12-01

    The seminiferous cycle of the rhinoceros was investigated for the first time using testicular tissue from captive white (Ceratotherium simum, n=2) and black (Diceros bicornis, n=1) rhinoceroses. Stages of the seminiferous epithelial cycle were characterised using the tubular morphology method and relative frequencies of each stage determined. This method allowed for the identification of eight stages of cellular associations characteristic of the seminiferous cycle in white and black rhinoceroses. The eight stages of the seminiferous cycle observed closely approximated the stages previously described for the domestic horse (Equus caballus). Premeiotic (stages I-III), meiotic (stage IV) and postmeiotic (stages V-VIII) represented 44.8%, 5.3% and 49.9% of the seminiferous cycle respectively. Copyright © 2012 Elsevier B.V. All rights reserved.

  2. Peptides from puff adder Bitis arietans venom, novel inhibitors of nicotinic acetylcholine receptors.

    Science.gov (United States)

    Vulfius, Catherine A; Spirova, Ekaterina N; Serebryakova, Marina V; Shelukhina, Irina V; Kudryavtsev, Denis S; Kryukova, Elena V; Starkov, Vladislav G; Kopylova, Nina V; Zhmak, Maxim N; Ivanov, Igor A; Kudryashova, Ksenia S; Andreeva, Tatyana V; Tsetlin, Victor I; Utkin, Yuri N

    2016-10-01

    Phospholipase A 2 (named bitanarin) possessing capability to block nicotinic acetylcholine receptors (nAChRs) was isolated earlier (Vulfius et al., 2011) from puff adder Bitis arietans venom. Further studies indicated that low molecular weight fractions of puff adder venom inhibit nAChRs as well. In this paper, we report on isolation from this venom and characterization of three novel peptides called baptides 1, 2 and 3 that reversibly block nAChRs. To isolate the peptides, the venom of B. arietans was fractionated by gel-filtration and reversed phase chromatography. The amino acid sequences of peptides were established by de novo sequencing using MALDI mass spectrometry. Baptide 1 comprised 7, baptides 2 and 3-10 amino acid residues, the latter being acetylated at the N-terminus. This is the first indication for the presence of such post-translational modification in snake venom proteins. None of the peptides contain cysteine residues. For biological activity studies the peptides were prepared by solid phase peptide synthesis. Baptide 3 and 2 blocked acetylcholine-elicited currents in isolated Lymnaea stagnalis neurons with IC 50 of about 50 μM and 250 μM, respectively. In addition baptide 2 blocked acetylcholine-induced currents in muscle nAChR heterologously expressed in Xenopus oocytes with IC 50 of about 3 μM. The peptides did not compete with radioactive α-bungarotoxin for binding to Torpedo and α7 nAChRs at concentration up to 200 μM that suggests non-competitive mode of inhibition. Calcium imaging studies on α7 and muscle nAChRs heterologously expressed in mouse neuroblastoma Neuro2a cells showed that on α7 receptor baptide 2 inhibited acetylcholine-induced increasing intracellular calcium concentration with IC 50 of 20.6 ± 3.93 μM. On both α7 and muscle nAChRs the suppression of maximal response to acetylcholine by about 50% was observed at baptide 2 concentration of 25 μM, the value being close to IC 50 on α7 nAChR. These data are

  3. Comparative studies of mitogen- and antigen-induced lymphocyte proliferation in four captive rhinoceros species.

    Science.gov (United States)

    Vance, Carrie K; Kennedy-Stoskopf, Suzanne; Obringer, Amy R; Roth, Terri L

    2004-12-01

    Cellular immune function in four rhinoceros species was evaluated by way of in vitro lymphocyte proliferation responses to mitogenic and antigenic stimuli to establish normative data on white blood cell activity for each species and to identify species-specific differences that might help explain the predisposition of black rhinoceroses (Diceros bicornis) to disease. A cross section of the U.S. rhinoceros population encompassing all four captive species was sampled, including the Sumatran rhinoceros (Dicerorhinus sumatrensis) (n = 3); Indian rhinoceros (Rhinoceros unicornis) (n = 4); African black rhinoceros (n = 16); and African white rhinoceros (Ceratotherium simum) (n = 10). Of the four species evaluated, African black rhinoceroses exhibited the weakest (P white rhinoceroses, Indian rhinoceroses, and Sumatran rhinoceroses. However, lymphocyte response to bacterial endotoxin lipopolysaccharide was similar (P > 0.05) across species. Antigenic stimulation produced much weaker responses than mitogenic stimulation. No differences (P > 0.05) were observed among rhinoceros species in response to 1 and 10 microg/ml of Leptospira icterohemorrhagiae or Leptospira gryppotyphosa. Lymphocytes from African white rhinoceroses proliferated weakly in the presence of Aspergillus fumigatus filtrate, whereas lymphocytes from the southern black rhinoceros subspecies appeared slightly suppressed in the presence of increasing doses (0.1, 1, and 10 microg/ml) of Aspergillus filtrate. This comparative data set characterizing lymphocyte proliferation in the rhinoceros reveals several differences in immune cell responses among rhinoceros species and provides some evidence that lymphocytes of captive African black rhinoceroses are less vigorous than those of the other rhinoceros species.

  4. Corticosteroid-induced suppression of in vitro lymphocyte proliferation in four captive rhinoceros species.

    Science.gov (United States)

    Roth, Terri L; Vance, Carrie K

    2007-12-01

    Captive African black rhinoceroses (Diceros bicornis) are unusually susceptible to several diseases not commonly observed in any of the other three rhinoceros species maintained in captivity. The potential role of corticosteroids (either endogenously produced or exogenously administered) in the development of these sometimes fatal diseases has been questioned. In this study, the suppressive effects of two therapeutic corticosteroids (dexamethasone and hydrocortisone) on in vitro lymphocyte proliferation was examined in four rhinoceros species, including the Sumatran rhinoceros (Dicerorhinus sumatrensis, n = 3), Indian rhinoceros (Rhinoceros unicornis, n = 4), African black rhinoceros (n = 10), and African white rhinoceros (Ceratotherium simum, n = 5). Three blood samples collected from each rhinoceros 1 mo to 1 yr apart provided replicates for the study. Both dexamethasone and hydrocortisone suppressed (P rhinoceros cell proliferation in response to any of the four mitogens was never completely suppressed, even in cultures containing the highest steroid concentration tested (10(-3) M). The effect of the two corticosteroids differed slightly among the rhinoceros species and subspecies tested, but there was no evidence that eastern or southern black rhinoceros lymphocytes were more sensitive to the suppressive effects of corticosteroids than the other rhinoceros species.

  5. Extraction of nuclear DNA from rhinoceros horn and characterization of DNA profiling systems for white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros.

    Science.gov (United States)

    Harper, Cindy K; Vermeulen, Gerhard J; Clarke, Amy B; de Wet, Jacobus I; Guthrie, Alan J

    2013-07-01

    Rhinoceros horn is now worth more, per unit weight, than gold, diamonds, or cocaine. Rhinoceros horn has been used in traditional Asian medicine as a presumed cure for a wide range of ailments. Rhinoceros poaching in South Africa has, on average, more than doubled each year over the past 5 years with the rapid economic growth in east and southeast Asia being assumed to be the primary factor driving the increased demand for horn. Here we report on the characterization of methods for genomic DNA extraction from rhinoceros horn and on DNA profiling systems for white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros. The DNA profiling system described includes 22 short tandem repeat (STR), or microsatellite, markers and a gender marker (ZF1), which have been used previously in various studies on rhinoceros. Using a θ value of 0.1, a conservative estimate of random match probability in 5 white rhinoceros ranged from 1:7.3x10(6) to 1:3.0x10(8). Given that the total population of white rhinoceros is approximately 20,000 such random match probabilities indicate that the genotyping system described provides data which can be used for evidentiary purposes. Furthermore, the methods are appropriate for use in investigations involving trace amounts of rhinoceros horn and the matching of profiles obtained from seized rhinoceros horn with material collected from live animals or poached carcasses. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  6. Effects of sociosexual environment on serum testosterone in captive male African rhinoceros.

    Science.gov (United States)

    Christensen, B W; Troedsson, M H T; Young, L J; Oliva, M; Penfold, L M

    2009-04-15

    The relationships between testosterone concentrations in male African rhinoceros and the presence of conspecific males and females were investigated. Serum testosterone concentrations were measured using enzyme-linked immunoassay (EIA) in 37 male black rhinoceros (Diceros bicornis) and 21 male white rhinoceros (Ceratotherium simum) housed at 37 institutions in the USA. Testosterone concentrations in both black (n=37) and white (n=21) rhinoceros males rose with increasing numbers of females present (Prhinoceros (Pwhite rhinoceros housed with other males. We inferred that introduction of females to a male may play an important role in stimulating libido and spermatogenesis. The similar response of black rhinoceros and white rhinoceros to increased numbers of females suggested that, at least historically, herd structure for blacks may have been more similar to whites than previously realized, and should be investigated further.

  7. On Rhinoceros simus, Burchell in the Leiden Museum

    NARCIS (Netherlands)

    Jentink, F.A.

    1890-01-01

    According to Dr. P. L. Sclater (Nature, September 25, 1890, p. 520) Mr. F. Selous says in the Field of August 16 as follows: — »it was within a mile of this spot that, two years previously (i. e. in 1888), I shot two white Rhinoceroses Rhinoceros simus), the last of their kind that have been killed

  8. Genetic variation studies in Oryctes rhinoceros (L.) (Coleoptera ...

    African Journals Online (AJOL)

    Randomly amplified microsatellite markers were used to study the genetic variation among six populations of Oryctes rhinoceros L. which were collected from oil palm plantations in Selangor, Perak, Pahang and Medan. Samples were collected using light and pheromone trapping for the purpose of obtaining two ...

  9. Feeding ecology of two endangered sympatric megaherbivores: Asian elephant Elephas maximus and greater one-horned rhinoceros Rhinoceros unicornis in lowland Nepal

    NARCIS (Netherlands)

    Pradhan, N.M.B.; Wegge, P.; Moe, S.R.; Shrestha, A.K.

    2008-01-01

    We studied the diets of low-density but increasing populations of sympatric Asian elephants Elephas maximus and greater one-horned rhinoceros Rhinoceros unicornis in the Bardia National Park in lowland Nepal. A microhistological technique based on faecal material was used to estimate the seasonal

  10. Tannin-binding salivary proteins in three captive rhinoceros species.

    Science.gov (United States)

    Clauss, Marcus; Gehrke, Janin; Hatt, Jean-Michel; Dierenfeld, Ellen S; Flach, Edmund J; Hermes, Robert; Castell, Johanna; Streich, W Juergen; Fickel, Joerns

    2005-01-01

    Tannin-binding salivary proteins (TBSP) are considered to be counter-defences acquired in the course of evolution by animals whose natural forage contains such tannins. As tannins mostly occur in browse material but not in grasses, it is assumed that grazers do not have a need for TBSP. Whereas it has been shown in several non-ungulate species that TBSP can be induced by dietary tannins, their presence or absence in ungulates has, so far, been shown to be a species-specific characteristic independent of dietary manipulations. We investigated saliva from three rhinoceros species from zoological gardens fed comparable, conventional zoo diets. As expected, saliva from white rhinoceroses (Ceratotherum simum, grazer) had lower tannin-binding capacities than that from black rhinoceroses (Diceros bicornis, browser). Surprisingly, however, Indian rhinoceroses (Rhinoceros unicornis), commonly regarded as grazers as well, displayed the highest tannin-binding capacities of the three species investigated. It is speculated that this discrepancy might be a result of an evolutionarily recent switch to a grass-dominated diet in Indian rhinoceroses, and that the black rhinoceros, which is closer related to the white rhinoceros than the Indian species, has evolved an inducible mechanism of TBSP production. In separate trials during which the tannin content of the diets of black rhinoceroses was increased by the addition of either tannic acid or quebracho, the tannin-binding capacity of black rhinoceros saliva was increased to levels within the same range as that of Indian rhinoceroses on the conventional diets. While induction trials in white and Indian rhinoceroses remain to be performed for a full understanding of salivary anti-tannin defence in rhinoceroses, these results are the first report of an induced salivary response to increased dietary tannin levels in an ungulate species.

  11. Epidemiology of Theileria bicornis among black and white rhinoceros metapopulation in Kenya

    OpenAIRE

    Otiende, Moses Y; Kivata, Mary W; Makumi, Joseph N; Mutinda, Mathew N; Okun, Daniel; Kariuki, Linus; Obanda, Vincent; Gakuya, Francis; Mijele, Dominic; Soriguer, Ram?n C; Alasaad, Samer

    2015-01-01

    Background A huge effort in rhinoceros conservation has focused on poaching and habitat loss as factors leading to the dramatic declines in the endangered eastern black rhinoceros (Diceros bicornis michaeli) and the southern white rhinoceros (Ceratotherium simum simum). Nevertheless, the role disease and parasite infections play in the mortality of protected populations has largely received limited attention. Infections with piroplasmosis caused by Babesia bicornis and Theileria bicornis has ...

  12. Features of reproduction and assisted reproduction in the white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros

    OpenAIRE

    Ververs, Cyrillus; van Zijl Langhout, M; Govaere, Jan; Van Soom, Ann

    2015-01-01

    Despite the worldwide increase of rhinoceros calf numbers, the growth of the population of white and black rhinoceros is slowing down mainly due to anthropogenic causes, such as poaching and habitat loss. Assisted reproduction is one of the methods of preserving the valuable genomes of these animals from being lost, and assists in breeding them in captivity to maintain the specie(s) numbers and provide an option for possible reintroduction into the wild. Since wild rhinoceros are difficult to...

  13. Butorphanol with oxygen insufflation corrects etorphine-induced hypoxaemia in chemically immobilized white rhinoceros (Ceratotherium simum)

    OpenAIRE

    Haw, Anna; Hofmeyr, Markus; Fuller, Andrea; Buss, Peter; Miller, Michele; Fleming, Gregory; Meyer, Leith

    2014-01-01

    Background Opioid-induced immobilization is associated with severe respiratory depression in the white rhinoceros. We evaluated the efficacy of butorphanol and oxygen insufflation in alleviating opioid-induced respiratory depression in eight boma-managed rhinoceros. Results Chemical immobilization with etorphine, azaperone and hyaluronidase, as per standard procedure for the white rhinoceros, caused severe respiratory depression with hypoxaemia (PaO2 = 27 ± 7 mmHg [mean ± SD]), hypercapnia (P...

  14. Putative filariosis outbreak in white and black rhinoceros at Meru National Park in Kenya

    OpenAIRE

    Mutinda, Matthew N.; Otiende, Moses; Gakuya, Francis; Kariuki, Linus; Obanda, Vincent; Ndeereh, David; Ndambiri, Ephantus; Kariuki, Edward; Lekolool, Isaac; Soriguer, Ramón C.; Rossi, Luca; Alasaad, Samer

    2012-01-01

    Abstract Background Habitat and food supply loss and disruption, together with man’s pursuit of the animal’s unique horn pose significant threats to the charismatic rhinoceros. Filarial worms have been thought to cause cutaneous lesions in black rhinoceros (Diceros bicornis) in Kenya and South Africa, but never in white rhinoceros (Ceratotherium simum) in the wild, despite the fact that the two species live often in close proximity. Stephanofilaria dinniki has been implicated in the past as t...

  15. Abstracts of papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988

    Directory of Open Access Journals (Sweden)

    Editorial Office

    1989-10-01

    Full Text Available The following abstracts is included: 1. Finances: the vital factor in rhinoceros conservation 2. Towards a black rhinoceros Diceros bicornis translocation strategy to meet the aims of the conservation plan for the species in South Africa and the TBVC states 3. Status, history and performance of black rhinoceros Diceros bicornis populations in South Africa, the TBVS states and Namibia 4. Distribution and monitoring of black rhinoceros Diceros bicornis populations in Zimbabwe 5. Census and marking systems for black rhinoceros Diceros bicornis with special reference to Zulu- land game reserves 6. The Auxiliary Game Guard System in northwestern Namibia and its role in black rhinoceros Diceros bicornis conservation 7. Black rhinoceros Diceros bicornis capture and translocation techniques as used in Etosha National Park 8. The role of non-governmental organisations in black rhinoceros Diceros bicornis conservation in Africa 9. Development of an intelligence/information network to counter poaching and the illegal trade in trophies

  16. Use of butorphanol during immobilization of free-ranging white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Miller, Michele; Buss, Peter; Joubert, Jenny; Mathebula, Nomkhosi; Kruger, Marius; Martin, Laura; Hofmeyr, Markus; Olea-Popelka, Francisco

    2013-03-01

    Forty free-ranging white rhinoceros (Ceratotherium simum) were anesthetized with etorphine, azaperone, and hyaluronidase in Kruger National Park, South Africa, between February and August 2009. Eighteen rhinoceros received butorphanol in the dart combination, and 22 rhinoceros had butorphanol administered intravenously within 15 min of darting. Body position, blood gas values, heart rate, respiratory rate, and temperature were measured at two time points after darting, approximately 10 min apart (sample 1 mean collection time after darting, 9.4 +/- 2.7 min; sample 2 mean collection time, 18.6 +/- 2.8 min). A significant number of field-captured rhinoceros remained standing at the first sample period when butorphanol was administered in the dart. Higher median values for arterial partial pressure of oxygen (PaO2) in combination with lower arterial partial pressure of carbon dioxide (PaCO2) in standing versus recumbent rhinoceros suggested improved ventilation in this posture (P rhinoceros that received butorphanol in the dart (P rhinoceros receiving butorphanol in the dart. Intravenous administration of butorphanol resulted in significantly decreased median PaCO2 and heart rate in recumbent rhinoceros (P rhinoceros remained hypoxemic during the short anesthetic procedure despite butorphanol administration. Preliminary results suggest that administration of butorphanol (either in the dart or intravenously) improves some metabolic parameters in free-ranging recumbent white rhinoceros without significantly affecting ventilation. It is hypothesized that this may be due to a lighter state of immobilization. Addition of butorphanol to the dart provides handling and physiologic advantages because the majority of rhinoceros remain standing.

  17. Castration of a Black Rhinoceros Diceros Bicornis Minor

    Directory of Open Access Journals (Sweden)

    V de Vos

    1980-12-01

    Full Text Available A black rhinoceros (Diceros bicornis minor in the Addo Elephant National Park was castrated in order to prevent the possibility of an aotic inducing gene to be introduced into the Addo population. The classic castration technique was used. It was subsequently found that the rhino showed a drastic change in behaviour, and is at this stage predictably timid, which is not the case with his testis carrying compeers.

  18. Castration of a Black Rhinoceros Diceros Bicornis Minor

    Directory of Open Access Journals (Sweden)

    V de Vos

    1980-01-01

    Full Text Available A black rhinoceros (Diceros bicornis minor in the Addo Elephant National Park was castrated in order to prevent the possibility of an aotic inducing gene to be introduced into the Addo population. The classic castration technique was used. It was subsequently found that the rhino showed a drastic change in behaviour, and is at this stage predictably timid, which is not the case with his testis carrying compeers.

  19. Neosporosis in a white rhinoceros (Ceratotherium simum calf

    Directory of Open Access Journals (Sweden)

    J.H. Williams

    2002-07-01

    Full Text Available A16-day-old white rhinoceros calf died suddenly while in excellent condition and showing no obvious previous clinical signs. It was the 9th calf of a mature female kept free-ranging with 11 other rhinoceros as well as various other game species on a 2000 hectare game breeding centre adjacent to the town of Lichtenburg and outlying cattle farmlands in the Northern Province. At post mortem examination, death was ascribed to heart failure. There was marked multifocal to coalescing subacute parasitic myocarditis with numerous proto-zoan bradyzoite cysts and free tachyzoites present amongst the predominantly round cell inflammatory infiltrate. The coccidian was positively identified as Neospora sp. using both polyclonal and murine monoclonal Neospora caninum antibody immunohistochemistry in the avidin-biotin technique. The parasites stained poorly with Toxoplasma gondii-specific immunoperoxidase staining. Ultrastructurally, a section of a bradyzoite-containing cyst, as well as tachyzoites, were largely but not totally consistent with those described for Neospora caninum. The dam showed no sign of illness. Neosporosis affecting white rhinoceros (Ceratotherium simum has not previously been reported. A summarised overview of neosporosis from selected publications and a recent review is given.

  20. Neosporosis in a white rhinoceros (Ceratotherium simum) calf.

    Science.gov (United States)

    Williams, J H; Espie, I; van Wilpe, E; Matthee, A

    2002-03-01

    A 16-day-old white rhinoceros calf died suddenly while in excellent condition and showing no obvious previous clinical signs. It was the 9th calf of a mature female kept free-ranging with 11 other rhinoceros as well as various other game species on a 2000 hectare game breeding centre adjacent to the town of Lichtenburg and outlying cattle farmlands in the Northern Province. At post mortem examination, death was ascribed to heart failure. There was marked multifocal to coalescing subacute parasitic myocarditis with numerous protozoan bradyzoite cysts and free tachyzoites present amongst the predominantly round cell inflammatory infiltrate. The coccidian was positively identified as Neospora sp. using both polyclonal and murine monoclonal Neospora caninum antibody immunohistochemistry in the avidin-biotin technique. The parasites stained poorly with Toxoplasma gondii-specific immunoperoxidase staining. Ultrastructurally, a section of a bradyzoite-containing cyst, as well as tachyzoites, were largely but not totally consistent with those described for Neospora caninum. The dam showed no sign of illness. Neosporosis affecting white rhinoceros (Ceratotherium simum) has not previously been reported. A summarised overview of neosporosis from selected publications and a recent review is given.

  1. Fat soluble vitamins in blood and tissues of free-ranging and captive rhinoceros.

    Science.gov (United States)

    Clauss, Marcus; Jessup, David A; Norkus, Edward B; Chen, Tai C; Holick, Michael F; Streich, W Juergen; Dierenfeld, Ellen S

    2002-04-01

    Several disease syndromes in captive rhinoceroses have been linked to low vitamin status. Blood samples from captive and free-ranging black (Diceros bicornis) and white rhinoceros (Ceratotherium simum) and tissue samples of captive individuals from four rhinoceros species were analysed for vitamins A and E. Circulating vitamin A levels measured as retinol for free-ranging versus captive black and white rhinoceros were 0.04 (+/- 0.03 SD) vs. 0.08 (+/- 0.08) and 0.07 (+/- 0.04) vs. 0.06 (+/- 0.02) microgram/ml, respectively. Circulating vitamin E levels measured as alpha-tocopherol were 0.58 (+/- 0.30) vs. 0.84 (+/- 0.96) and 0.62 (+/- 0.48) vs. 0.77 (+/- 0.32) microgram/ml, respectively. In contrast to earlier findings, there was no significant difference in vitamin E concentration between captive and free-ranging black rhinoceros. When the samples of captive black rhinoceros were grouped into those taken before 1990 and after 1990, however, those collected before 1990 had significantly lower (P white rhinoceroses appear to be adequately supplemented in vitamin A and E. Captive Indian rhinoceroses (Rhinoceros unicornis) had significantly lower vitamin A concentrations in blood (P rhinoceros species. Equine requirements are not recommended as a model for rhinoceros vitamin requirements.

  2. CARDIOVASCULAR EFFECTS OF ETORPHINE, AZAPERONE, AND BUTORPHANOL COMBINATIONS IN CHEMICALLY IMMOBILIZED CAPTIVE WHITE RHINOCEROS (CERATOTHERIUM SIMUM).

    Science.gov (United States)

    Buss, Peter; Miller, Michele; Fuller, Andrea; Haw, Anna; Wanty, Rachel; Olea-Popelka, Francisco; Meyer, Leith

    2016-09-01

    Chemical capture is an essential tool in the management and conservation of white rhinoceros ( Ceratotherium simum ); however, cardiovascular responses in immobilized megaherbivores are poorly understood. Blood pressure and heart rate responses in rhinoceros immobilized with etorphine or etorphine plus azaperone, and the effects of subsequent i.v. butorphanol administration were investigated. Six white rhinoceros were used in a randomized crossover study design with four interventions: 1) etorphine i.m.; 2) etorphine plus azaperone i.m.; 3) etorphine i.m. and butorphanol i.v.; and 4) etorphine plus azaperone i.m., and butorphanol i.v. Etorphine resulted in hypertension and tachycardia in immobilized rhinoceros on initial measurements. Over the 25-min study period, blood pressures and heart rate declined. Heart rates were slower, although the rhinoceros were still tachycardic, and blood pressures lower during the whole study period in animals immobilized with etorphine and azaperone compared with those that received only etorphine. Butorphanol administration resulted in lower arterial blood pressures and heart rates in etorphine-immobilized rhinoceros. In rhinoceros immobilized with etorphine and azaperone, heart rate slowed following administration of butorphanol i.v., although blood pressures remained unchanged. Azaperone reduced hypertension associated with etorphine immobilization, but animals remained tachycardic. Administration of butorphanol to etorphine/azaperone-immoblized rhinoceros lowered heart rate to values approaching normal resting levels without altering blood pressure.

  3. Death due to volvulus in a white rhinoceros ceratotherium simum from the Kruger National Park

    Directory of Open Access Journals (Sweden)

    V. de Vos

    1975-07-01

    Full Text Available Acute intestinal obstruction due to volvulus is described as the cause of death in an adult white rhinoceros cow. It is also pointed out that the gross anatomical features which predispose volvulus in the horse, are also present in the white rhinoceros and is considered to have some significance in the aetiology of the present case.

  4. A bibliography of black rhinoceros Diceros bicomis (Linnaeus, 1758) and white rhinoceros Ceratotherium simum (Burchell, 1817) for southern Africa

    OpenAIRE

    Heather Wildi

    1989-01-01

    A general bibliography of reference material relevant to wildlife managers and researchers involved in the conservation of black and white rhinoceros in southern Africa is provided. It includes both key word and full reference listings.

  5. Serum iron and selected biochemical values in free-ranging black rhinoceros (Diceros bicornis) from South Africa.

    Science.gov (United States)

    Miller, Michele; Olea-Popelka, Francisco; Joubert, Jennifer; Mathebula, Nomkhosi; Zimmerman, David; Hausler, Guy; Dreyer, Cathy; Hofmeyr, Markus; Buss, Peter

    2012-09-01

    Serum samples collected from 20 black rhinoceros (Diceros bicornis) were analyzed for iron values from six different areas in South Africa. In addition, biochemical profiles were performed on individual samples. Comparisons of iron values from free-ranging black rhinoceros and from 28 free-ranging white rhinoceros (Ceratotherium simum) were conducted by location and age. Among the free-ranging black rhinoceros, samples were compared from different regions to a set of samples from black rhinoceros that had been captured and held in bomas. Serum iron levels were not significantly different (P = 0.55) among the three locations with more than one animal (medians 5.57, 5.70, 6.47 ppm), but the median value from the boma group was significantly lower (2.91 ppm; P = 0.042), contrary to previous studies. Similar to reports in captive black rhinos, serum iron levels appeared to show a trend toward increasing values between subadult and adult animals, although differences were not statistically significant among black rhinoceros. Comparison of serum iron levels between free-ranging black and white rhinoceros showed significantly higher median value in black rhinoceros (5.73 ppm) versus white rhinoceros (3.38 ppm, P= 0.001). Other significant differences (P rhinoceros. Further investigations should be conducted to examine the role of age, location, and time in boma confinement on iron values in South African rhinoceros to understand iron metabolism in these species.

  6. Mitochondrial DNA Phylogenetics of Black Rhinoceros in Kenya in relation to Southern Africa Population

    Directory of Open Access Journals (Sweden)

    Elijah K. Githui

    2017-01-01

    Full Text Available Black rhinoceros (Diceros bicornis are highly endangered due to poaching and other anthropological reasons and their protection to rebound the numbers and genetic improvement are necessary remedial measures defined by Rhino International Union of Conservation for the Nature Red List (IUCN. In Kenya black rhino numbers declined from approximately 20,000 in the 1970s to fewer than 400 in 1982. Wildlife conservation managers effected strategies to manage/breed the remaining rhinoceros populations in Eastern and Southern Africa within regional sanctuaries. This study analyzes the genetic variability of these remnant rhinoceros using Mitochondrial DNA (mtDNA. Majority of the rhinoceros in both Kenyan and Southern Africa group are monophyletic clusters with insignificant genetic variations while some lineages are underrepresented. The Eastern Africa rhinoceros forms a distinct clade from the Sothern Africa counterpart while Tanzania population has admixtures. Tajima-D test showed that these two populations are under different selection pressure possibly due to different history of adverse anthropologic activities. Similarly, the Southern Africa rhinoceros have low genetic diversity compared to the Eastern African population due to extended periods of game hunting during Africa colonization. This study suggests that managed translocations of individual rhinoceros across the separated fragments can be applied to improve their genetic diversity.

  7. Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-gamma) and the production of rhinoceros IFN-gamma specific antibodies.

    Science.gov (United States)

    Morar, D; Tijhaar, E; Negrea, A; Hendriks, J; van Haarlem, D; Godfroid, J; Michel, A L; Rutten, V P M G

    2007-01-15

    Bovine tuberculosis (BTB) is endemic in African buffalo (Syncerus caffer) in the Kruger National Park (KNP). In addition to buffalo, Mycobacterium bovis has been found in at least 14 other mammalian species in South Africa, including kudu (Tragelaphus strepsiceros), Chacma baboon (Papio ursinus) and lion (Panthera leo). This has raised concern about the spillover into other potentially susceptible species like rhinoceros, thus jeopardising breeding and relocation projects aiming at the conservation of biodiversity. Hence, procedures to screen for and diagnose BTB in black rhinoceros (Diceros bicornis) and white rhinoceros (Ceratotherium simum) need to be in place. The Interferon-gamma (IFN-gamma) assay is used as a routine diagnostic tool to determine infection of cattle and recently African buffalo, with M. bovis and other mycobacteria. The aim of the present work was to develop reagents to set up a rhinoceros IFN-gamma (RhIFN-gamma) assay. The white rhinoceros IFN-gamma gene was cloned, sequenced and expressed as a mature protein. Amino acid (aa) sequence analysis revealed that RhIFN-gamma shares a homology of 90% with equine IFN-gamma. Monoclonal antibodies, as well as polyclonal chicken antibodies (Yolk Immunoglobulin-IgY) with specificity for recombinant RhIFN-gamma were produced. Using the monoclonals as capture antibodies and the polyclonal IgY for detection, it was shown that recombinant as well as native white rhinoceros IFN-gamma was recognised. This preliminary IFN-gamma enzyme-linked immunosorbent assay (ELISA), has the potential to be developed into a diagnostic assay for M. bovis infection in rhinoceros.

  8. Histological Study of the Adrenal Gland of African White Rhinoceros

    Directory of Open Access Journals (Sweden)

    Ke-mei Peng§*, Hui Song§, Hua-zhen Liu, Jian-bin Zhang1, Zhi-qiang Lu, Zhi-wei Liu and Yin-xue Liu

    2012-06-01

    Full Text Available The microstructures of adrenal gland of white rhinoceros (Ceratotherium simum were observed by light microscopy. The results showed that the surface envelope of adrenal gland was covered with developed connective tissues whose average thickness was up to 210 μm. A large number of sinusoids were distributed between the neighboring cells. The zona glomerulosa of adrenal cortex was thin and composed of columnar and cubic cells. The columnar cells were close to the edge and the cubic cells were found in the deep zone. The two kinds of cells were arranged in irregular groups. The thickness of zona fasiculata was about 3 times as much as that of zona glomerulosa, and the boundary was not obvious. Cells were arranged in irregular cords. Some small lipid droplets were in the shape of small vacuolation, and distributed in the cytoplasm. The cells of zona reticularis were adjacent to medulla with which they formed a jagged boundary. Cells were in network or in enclose acini. Medullae were darkly stained and clearly distinct from the cortex. Cells were large with elliptical nuclei whose nucleoli were significant. Cytoplasm was basophilic and stained to give blue violet appearance. A small amount of sympathetic ganglion cells were distributed in the medulla. The above results suggested that the adrenal gland structures of African white rhinoceros compared with other animals have similarities. . The rich sinusoids in capsule suggested that its blood supply was abundant, full of metabolism and endocrine activity.

  9. Baseline Hematologic Results for Free-ranging White Rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.

    Science.gov (United States)

    Miller, Michele; Buss, Peter; Wanty, Rachel; Parsons, Sven; van Helden, Paul; Olea-Popelka, Francisco

    2015-10-01

    Complete blood counts (n = 115) and red blood cell analytes (n = 80) were assessed in free-ranging white rhinoceros (Ceratotherium simum) from Kruger National Park (KNP), South Africa. Blood was collected from healthy animals immobilized between February and September 2009-11 for management purposes. Our objectives were to establish baselines for KNP's white rhinoceros population and to compare results based on sex and age group. Significant differences in total white blood cells, total eosinophils, and hemoglobin were found between adult and subadult rhinoceros. Female rhinoceros had significantly higher total white blood cells and lower hemoglobin compared with males. Hematologic analytes were similar to those published for other rhinoceros populations, although the impact of capture and sampling methods, nutritional status, and habitat should be considered when comparing data. This baseline hematology for healthy free-ranging white rhinoceros in KNP may be useful in assessing health status for translocation and medical interventions, including treatment of poaching cases.

  10. Landscape preference of the white rhinoceros in the southern Kruger National Park

    Directory of Open Access Journals (Sweden)

    D.J. Pienaar

    1992-09-01

    Full Text Available The long and short-term landscape preference of white rhinoceros in the southern Kruger NationarPark are investigated. A preference index and a chi-square test are used to ascertain if white rhinoceros prefer or avoid a particular landscape. Landscape 3 (moderately undulating granitoid plains with Combretnni zeyheri woodland, is the most preferred landscape while landscapes 2 (low granitoid mountains with Combretnmapicniafnni bushveld and4 (granitoid lowlands with Acacia ^randicornuta tree- savanna, are avoided.

  11. Radio transmitter implants in the horns of both the white and the black rhinoceros in the Kruger National Park

    Directory of Open Access Journals (Sweden)

    D.J. Pienaar

    1991-09-01

    Full Text Available The procedure for implanting radio transmitters into the horns of white and black rhinoceroses is described. Mean transmitter life in the white rhinoceros was 13,9 months which is significantly longer than the 9,7 months in black rhinoceros. In the white rhinoceros a significant sex-related difference in transmitter life was found with the transmitters in males lasting a mean of 12,1 months compared to the 15,3 months in females.

  12. Radio transmitter implants in the horns of both the white and the black rhinoceros in the Kruger National Park

    OpenAIRE

    D.J. Pienaar; A.J. Hall-Martin

    1991-01-01

    The procedure for implanting radio transmitters into the horns of white and black rhinoceroses is described. Mean transmitter life in the white rhinoceros was 13,9 months which is significantly longer than the 9,7 months in black rhinoceros. In the white rhinoceros a significant sex-related difference in transmitter life was found with the transmitters in males lasting a mean of 12,1 months compared to the 15,3 months in females.

  13. Identification of policies for a sustainable legal trade in rhinoceros horn based on population projection and socioeconomic models

    OpenAIRE

    Di Minin, Enrico; Laitila, Jussi; Montesino Pouzols, Federico; Leader-Williams, Nigel; Slotow, Rob; Goodman, Peter; Conway, Anthony; Moilanen, Atte

    2015-01-01

    Between 1990 and 2007, 15 southern white (Ceratotherium simum simum) and black (Diceros bicornis) rhinoceroses on average were killed illegally every year in South Africa. Since 2007 illegal killing of southern white rhinoceros for their horn has escalated to >950 individuals/year in 2013. We conducted an ecological-economic analysis to determine whether a legal trade in southern white rhinoceros horn could facilitate rhinoceros protection. Generalized linear models were used to examine the s...

  14. Assisted reproduction in female rhinoceros and elephants--current status and future perspective.

    Science.gov (United States)

    Hermes, R; Göritz, F; Streich, Wj; Hildebrandt, Tb

    2007-09-01

    Over the last few decades, rhinoceroses and elephants became important icons in the saga of wildlife conservation. Recent surveys estimate the wild Asian (Elephas maximus) and African (Loxodonta africana) elephant populations to be, at most, 50 250 and 637 600 respectively. For the five rhinoceros species, black (Diceros bicornis), white (Ceratotherium simum), Indian (Rhinoceros unicornis), Javan (Rhinoceros sondaicus) and Sumatran rhinoceros (Dicerorhinus Sumatrensis), the population estimates of 3610, 11 330, 2400, 60 and 300, respectively, are of even greater concern. Protected against habitat loss, poaching and left undisturbed, rhinoceros and elephants reproduce well in the wild. But small and decreasing populations make successful captive management of these taxa increasingly important. In captivity, however, most populations face possible 'extinction' because of historically poor reproductive performance. From the first descriptions of the reproductive anatomy and the oestrous cycle (Laws 1969; Kassam and Lasley 1981; Balke et al. 1988a,b; Plotka et al. 1988; Godfrey et al. 1991) to the present use of advanced assisted reproduction technologies, researchers have strive to understand the function and dysfunction of the reproductive biology of these charismatic species. This paper reviewed the current knowledge on rhinoceros and elephant reproduction biology, reproductive cycle, gestation, dystocia, reproductive pathology, oestrous induction and artificial insemination, sperm sexing, IVF and contraception, and how this knowledge is or might be used to aid species conservation for maximal reproductive efficiency and enhancement of genetic management.

  15. First insights into the vocal repertoire of infant and juvenile Southern white rhinoceros.

    Science.gov (United States)

    Linn, Sabrina N; Boeer, Michael; Scheumann, Marina

    2018-01-01

    Describing vocal repertoires represents an essential step towards gaining an overview about the complexity of acoustic communication in a given species. The analysis of infant vocalisations is essential for understanding the development and usage of species-specific vocalisations, but is often underrepresented, especially in species with long inter-birth intervals such as the white rhinoceros. Thus, this study aimed for the first time to characterise the infant and juvenile vocal repertoire of the Southern white rhinoceros and to relate these findings to the adult vocal repertoire. The behaviour of seven mother-reared white rhinoceros calves (two males, five females) and one hand-reared calf (male), ranging from one month to four years, was simultaneously audio and video-taped at three zoos. Normally reared infants and juveniles uttered four discriminable call types (Whine, Snort, Threat, and Pant) that were produced in different behavioural contexts. All call types were also uttered by the hand-reared calf. Call rates of Whines, but not of the other call types, decreased with age. These findings provide the first evidence that infant and juvenile rhinoceros utter specific call types in distinct contexts, even if they grow up with limited social interaction with conspecifics. By comparing our findings with the current literature on vocalisations of adult white rhinoceros and other solitary rhinoceros species, we discuss to which extent differences in the social lifestyle across species affect acoustic communication in mammals.

  16. Putative filariosis outbreak in white and black rhinoceros at Meru National Park in Kenya

    Science.gov (United States)

    2012-01-01

    Background Habitat and food supply loss and disruption, together with man’s pursuit of the animal’s unique horn pose significant threats to the charismatic rhinoceros. Filarial worms have been thought to cause cutaneous lesions in black rhinoceros (Diceros bicornis) in Kenya and South Africa, but never in white rhinoceros (Ceratotherium simum) in the wild, despite the fact that the two species live often in close proximity. Stephanofilaria dinniki has been implicated in the past as the causal agents for such lesions. Findings In this paper we report a putative filariosis outbreak in both black and white rhinos at Meru National Park in Kenya. Four black and five white rhinos were affected by various degrees of filarioid-like lesions, while apparently all sympatric wild and domestic animals were filarial worm-free. Affected rhinos were captured and successfully treated. Comparison between the epidemiological aspects of white and black rhinoceros filariosis, and the possible relations between this outbreak and annual seasons, the presence of oxpeckers and other host species are discussed. Conclusions Our study highlights (i) that filarial infection is not restricted to black rhinos, but it affects both rhinoceros species, and (ii) the importance of the earlier detection and immediate treatment (capture-treat and release) of filarioid infections, which is of pivotal interest for wildlife conservation, and especially the endangered and isolated white and black rhinoceros populations. PMID:22992297

  17. Putative filariosis outbreak in white and black rhinoceros at Meru National Park in Kenya.

    Science.gov (United States)

    Mutinda, Matthew; Otiende, Moses; Gakuya, Francis; Kariuki, Linus; Obanda, Vincent; Ndeere, David; Ndambiri, Ephantus; Kariuki, Edward; Lekolool, Isaac; Soriguer, Ramón C; Rossi, Luca; Alasaad, Samer

    2012-09-19

    Habitat and food supply loss and disruption, together with man's pursuit of the animal's unique horn pose significant threats to the charismatic rhinoceros. Filarial worms have been thought to cause cutaneous lesions in black rhinoceros (Diceros bicornis) in Kenya and South Africa, but never in white rhinoceros (Ceratotherium simum) in the wild, despite the fact that the two species live often in close proximity. Stephanofilaria dinniki has been implicated in the past as the causal agents for such lesions. In this paper we report a putative filariosis outbreak in both black and white rhinos at Meru National Park in Kenya. Four black and five white rhinos were affected by various degrees of filarioid-like lesions, while apparently all sympatric wild and domestic animals were filarial worm-free. Affected rhinos were captured and successfully treated. Comparison between the epidemiological aspects of white and black rhinoceros filariosis, and the possible relations between this outbreak and annual seasons, the presence of oxpeckers and other host species are discussed. Our study highlights (i) that filarial infection is not restricted to black rhinos, but it affects both rhinoceros species, and (ii) the importance of the earlier detection and immediate treatment (capture-treat and release) of filarioid infections, which is of pivotal interest for wildlife conservation, and especially the endangered and isolated white and black rhinoceros populations.

  18. Putative filariosis outbreak in white and black rhinoceros at Meru National Park in Kenya

    Directory of Open Access Journals (Sweden)

    Mutinda Matthew

    2012-09-01

    Full Text Available Abstract Background Habitat and food supply loss and disruption, together with man’s pursuit of the animal’s unique horn pose significant threats to the charismatic rhinoceros. Filarial worms have been thought to cause cutaneous lesions in black rhinoceros (Diceros bicornis in Kenya and South Africa, but never in white rhinoceros (Ceratotherium simum in the wild, despite the fact that the two species live often in close proximity. Stephanofilaria dinniki has been implicated in the past as the causal agents for such lesions. Findings In this paper we report a putative filariosis outbreak in both black and white rhinos at Meru National Park in Kenya. Four black and five white rhinos were affected by various degrees of filarioid-like lesions, while apparently all sympatric wild and domestic animals were filarial worm-free. Affected rhinos were captured and successfully treated. Comparison between the epidemiological aspects of white and black rhinoceros filariosis, and the possible relations between this outbreak and annual seasons, the presence of oxpeckers and other host species are discussed. Conclusions Our study highlights (i that filarial infection is not restricted to black rhinos, but it affects both rhinoceros species, and (ii the importance of the earlier detection and immediate treatment (capture-treat and release of filarioid infections, which is of pivotal interest for wildlife conservation, and especially the endangered and isolated white and black rhinoceros populations.

  19. Germany/Australia index of sperm sex sortability in elephants and rhinoceros.

    Science.gov (United States)

    Behr, B; Rath, D; Hildebrandt, T B; Goeritz, F; Blottner, S; Portas, T J; Bryant, B R; Sieg, B; Knieriem, A; de Graaf, S P; Maxwell, W M C; Hermes, R

    2009-04-01

    Flow cytometric sexing of spermatozoa followed by application in artificial insemination or in vitro fertilization provides a unique opportunity to predetermine the sex of offspring and might enhance the conservation management of endangered species in captivity such as the elephant and rhinoceros. To obtain an indication of the sortability of spermatozoa from these species, the relative DNA differences between X and Y chromosome bearing spermatozoa (fresh, frozen thawed, epididymal) from three rhinoceros species [white (Ceratotherium simum), black (Diceros bicornis), Indian (Rhinoceros unicornis)] and both elephant species, the Asian and the African elephant (Elephas maximus, Loxodonta Africana), were determined through separation of spermatozoa into X and Y chromosome bearing populations, using a modified high speed flow cytometer. The head profile areas of spermatozoa from all five species were measured using light microscopy. By multiplying the relative DNA differences and the head profile areas, the sperm sorting indices were calculated to be 47, 48 and 51 for white, black and Indian rhinoceros respectively. The calculated sorting index for the Asian elephant was 66. In the African elephant, we determined the highest sorting index of 76. These results indicate the practicability of flow cytometric sex sorting of spermatozoa from the tested rhinoceros species and both elephant species. The lower sorting indices in rhinos indicate that sex sorting of spermatozoa from the rhinoceros will be more challenging than in elephants.

  20. Prey preference of Myopopone castanea (hymenoptera: formicidae) toward larvae Oryctes rhinoceros Linn (coleoptera: scarabidae)

    Science.gov (United States)

    Widihastuty; Tobing, M. C.; Marheni; Kuswardani, R. A.

    2018-02-01

    Myopoponecastanea (Hymenoptera: Formicidae) ant is a predator for larvae Oryctes rhinoceros (Coleoptera: Scarabidae) which is a pest on oil palm. These ants are able to prey on all stadia of O. rhinoceros larvae. This study was conducted to determine prey preference of M. castaneatoward its prey O. rhinoceros larvae.. The study was conducted using a Factorial Complete Random Design with two factors (using log and no log) and five replications. Preferences test was done by choice test and no choice test. The results of no choice preference test on the log treatment, M. castaneaprefer preyed on firstinstar larvae of O. rhinoceros (\\bar{X}=2.6 individual) with a preference index was 0.194 and on no log treatment, M. castanea prefer for both first instar larvae and second instarlarvae (\\bar{X}=4.6 individual) with a preference index 0.197. The results of the choice preference test using logs, showed that M. castanea prefer the firstinstar larvae of O. rhinoceros (\\bar{X}=2.6 individual), with a preference index (0.35), and on no log treatment, M. castaneaprefer the second instar larvae(\\bar{X}=1.4 individual) with a preference index 0.189. Both first and secondinstarlarvaeof O. rhinoceros were preferred by predator M. castanea

  1. Carcinosarcoma in a white Rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Suzuki, Takuya; Kudo, Tomoo; Kawakami, Shigehisa; Une, Yumi

    2013-12-30

    In Rhinocerotidae, there are very few reports of tumors and no reports of a mixed tumor. This paper reports the case of a male 33-year-old southern white rhinoceros. Grossly, there were two masses in the coelomic cavity and solid nodules in the liver. Histologically, all tumors had a biphasic pattern that consisted of malignant epithelial cells (cytokeratin- and E-cadherin-positive) and non-epithelial cells (vimentin-positive) with cartilage. In this case, the prostate could not be identified, and instead, the largest tumor mass was present at that site. Furthermore, since structures regarded as the prostate duct remained in this tumor, we considered that this tumor was very likely to be of prostate gland origin. This case is the first report of carcinosarcoma in Rhinocerotidae.

  2. The identification of Theileria bicornis in captive rhinoceros in Australia

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    Jerald Yam

    2018-04-01

    Full Text Available Poaching of both black (Diceros bicornis and white (Ceratotherium simum rhinoceros in Africa has increased significantly in recent years. In an effort to ensure the survival of these critically endangered species, breeding programs were established in the 1990s in Australia, where a similar climate and habitat is available. In this study we examined blood samples from two C. simum, including a 16 yr old female (Aluka who died in captivity, and a 17 yr old asymptomatic male (Umfana. Bloods from seven healthy D. bicornis housed at the zoo were also collected. All samples were tested for the presence of piroplasms via blood smear and PCR. A generic PCR for the 18S rRNA gene of the Piroplasmida revealed the presence of piroplasm infection in both dead and asymptomatic C. simum. Subsequent sequencing of these amplicons revealed the presence of Theileria bicornis. Blood smear indicated that this organism was present at low abundance in both affected and asymptomatic individuals and was not linked to the C. simum mortality. T. bicornis was also detected in the D. bicornis population (n = 7 housed at Taronga Western Plains Zoo using PCR and blood film examination; however only animals imported from Africa (n = 1 tested T. bicornis positive, while captive-born animals bred within Australia (n = 6 tested negative suggesting that transmission within the herd was unlikely. Phylogenetic analysis of the full length T. bicornis 18S rRNA genes classified this organism outside the clade of the transforming and non-transforming Theileria with a new haplotype, H4, identified from D. bicornis. This study revealed the presence of Theileria bicornis in Australian captive populations of both C. simum and D. bicornis and a new haplotype of the parasite was identified. Keywords: Piroplasm, Theileria bicornis, 18S rRNA, Rhinoceros, Translocation

  3. Determining Visitor Preferences for Rhinoceros Conservation Management at Private, Ecotourism Game Reserves in the Eastern Cape Province, South Africa: A Choice Modeling Experiment

    OpenAIRE

    Deborah E Lee and Mario Du Preez

    2015-01-01

    South Africa harbours 95 percent of the world’s threatened white rhinoceros (18 000) population and 40 percent of the critically endangered black rhinoceros (1 950) population. Increased levels of rhinoceros poaching in South Africa, and the imminent threat of extinction, has emphasized the need for improved management and conservation policies. This pilot study employs a discrete choice experiment in order to value selected supply-side rhinoceros management and conservation strategies at p...

  4. Identification of policies for a sustainable legal trade in rhinoceros horn based on population projection and socioeconomic models.

    Science.gov (United States)

    Di Minin, Enrico; Laitila, Jussi; Montesino-Pouzols, Federico; Leader-Williams, Nigel; Slotow, Rob; Goodman, Peter S; Conway, Anthony J; Moilanen, Atte

    2015-04-01

    Between 1990 and 2007, 15 southern white (Ceratotherium simum simum) and black (Diceros bicornis) rhinoceroses on average were killed illegally every year in South Africa. Since 2007 illegal killing of southern white rhinoceros for their horn has escalated to >950 individuals/year in 2013. We conducted an ecological-economic analysis to determine whether a legal trade in southern white rhinoceros horn could facilitate rhinoceros protection. Generalized linear models were used to examine the socioeconomic drivers of poaching, based on data collected from 1990 to 2013, and to project the total number of rhinoceroses likely to be illegally killed from 2014 to 2023. Rhinoceros population dynamics were then modeled under 8 different policy scenarios that could be implemented to control poaching. We also estimated the economic costs and benefits of each scenario under enhanced enforcement only and a legal trade in rhinoceros horn and used a decision support framework to rank the scenarios with the objective of maintaining the rhinoceros population above its current size while generating profit for local stakeholders. The southern white rhinoceros population was predicted to go extinct in the wild rhinoceros population above its current size was to provide a medium increase in antipoaching effort and to increase the monetary fine on conviction. Without legalizing the trade, implementing such a scenario would require covering costs equal to approximately $147,000,000/year. With a legal trade in rhinoceros horn, the conservation enterprise could potentially make a profit of $717,000,000/year. We believe the 35-year-old ban on rhinoceros horn products should not be lifted unless the money generated from trade is reinvested in improved protection of the rhinoceros population. Because current protection efforts seem to be failing, it is time to evaluate, discuss, and test alternatives to the present policy. © 2014 The Authors. Conservation Biology published by wiley

  5. Three Novel Haplotypes of Theileria bicornis in Black and White Rhinoceros in Kenya.

    Science.gov (United States)

    Otiende, M Y; Kivata, M W; Jowers, M J; Makumi, J N; Runo, S; Obanda, V; Gakuya, F; Mutinda, M; Kariuki, L; Alasaad, S

    2016-02-01

    Piroplasms, especially those in the genera Babesia and Theileria, have been found to naturally infect rhinoceros. Due to natural or human-induced stress factors such as capture and translocations, animals often develop fatal clinical piroplasmosis, which causes death if not treated. This study examines the genetic diversity and occurrence of novel Theileria species infecting both black and white rhinoceros in Kenya. Samples collected opportunistically during routine translocations and clinical interventions from 15 rhinoceros were analysed by polymerase chain reaction (PCR) using a nested amplification of the small subunit ribosomal RNA (18S rRNA) gene fragments of Babesia and Theileria. Our study revealed for the first time in Kenya the presence of Theileria bicornis in white (Ceratotherium simum simum) and black (Diceros bicornis michaeli) rhinoceros and the existence of three new haplotypes: haplotypes H1 and H3 were present in white rhinoceros, while H2 was present in black rhinoceros. No specific haplotype was correlated to any specific geographical location. The Bayesian inference 50% consensus phylogram recovered the three haplotypes monophyleticly, and Theileria bicornis had very high support (BPP: 0.98). Furthermore, the genetic p-uncorrected distances and substitutions between T. bicornis and the three haplotypes were the same in all three haplotypes, indicating a very close genetic affinity. This is the first report of the occurrence of Theileria species in white and black rhinoceros from Kenya. The three new haplotypes reported here for the first time have important ecological and conservational implications, especially for population management and translocation programs and as a means of avoiding the transport of infected animals into non-affected areas. © 2014 Blackwell Verlag GmbH.

  6. Pengaruh Warna Perangkap Feromon terhadap Hasil Tangkapan Imago Oryctes rhinoceros di Perkebunan Kelapa Sawit

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    Idum Satia Santi

    2008-12-01

    Full Text Available The effectiveness of a synthetic-pheromone trap with active ingredient of ethyl-4-methyloctanoate in catching adults of Oryctes rhinoceros was believed to be influenced by the trap color. A RCBD trial with single factor and three block replicates was conducted during September to October 2008 at Oil-Palm-Trial Station at PTPN III, Labuhan Batu District, North-Sumatera Province. The single factor was trap color i.e. orange, green, white, blue, red, yellow, and pink. Results showed that total numbers and sex ratios of collected O. rhinoceros adults were not significantly influenced by the trap color. The total numbers of collected O. rhinoceros adult during 30 days on color of orange, green, white, blue, red, yellow, and pink were 136, 177, 126, 155, 129, 114, and 113 individuals with sex ratios ♂/♀ 0.47, 0.52, 0.62, 0.50, 0.60, 0.49, and 0.54, respectively. The synthetic pheromone significantly attracted more O. rhinoceros female (65% rather than the male one (35%.   Keefektifan perangkap feromon sintetik berbahan aktif ethyl-4-methyloctanoate dalam menangkap imago Oryctes rhinoceros diyakini dipengaruhi oleh warna perangkap tersebut. Percobaan RCBD faktor tunggal dengan tiga blok ulangan dilakukan pada bulan September sampai dengan Oktober 2008 di kebun penelitian kelapa sawit PTPN III, Kabupaten Labuhan Batu, Provinsi Sumatera Utara. Faktor tunggal tersebut yakni warna perangkap meliputi oranye, hijau, putih, biru, merah, kuning, dan pink. Hasil penelitian menunjukkan bahwa warna perangkap tidak berpengaruh signifikan terhadap hasil tangkapan dan sex ratio imago O. rhinoceros. Total imago O. rhinoceros yang tertangkap selama 30 hari pada warna oranye, hijau, putih, biru, merah, kuning dan pink sebanyak 136, 177, 126, 155, 129, 114 dan 113 dengan sex ratio ♂/♀ 0,47; 0,52; 0,62; 0,50; 0,60; 0,49 dan 0,54. Feromon sintetik secara signifikan lebih banyak menarik imago betina (65% daripada yang jantan (35%.

  7. A rapid chemical odour profiling method for the identification of rhinoceros horns.

    Science.gov (United States)

    Ueland, Maiken; Ewart, Kyle; Troobnikoff, Amanda N; Frankham, Greta; Johnson, Rebecca N; Forbes, Shari L

    2016-09-01

    Illegal poaching causes great harm to species diversity and conservation. A vast amount of money is involved in the trade of illegal or forged animal parts worldwide. In many cases, the suspected animal part is unidentifiable and requires costly and invasive laboratory analysis such as isotopic fingerprinting or DNA testing. The lack of rapid and accurate methods to identify wildlife parts at the point of detection represents a major hindrance in the enforcement and prosecution of wildlife trafficking. The ability of wildlife detector dogs to alert to different wildlife species demonstrates that there is a detectable difference in scent profile of illegally traded animal parts. This difference was exploited to develop a rapid, non-invasive screening method for distinguishing rhinoceros horns of different species. The method involved the collection of volatile organic compounds (VOC) by headspace solid-phase microextraction (HS-SPME) and analysis by comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (GC×GC-TOFMS). It was hypothesised that the use of the specific odour profile as a screening method could separate and differentiate geographic origin or exploit the difference in diets of different species within a family (such as white rhinoceros and black rhinoceros from the Rhinocerotidae family). Known black and white rhinoceros horn samples were analysed using HS-SPME-GC×GC-TOFMS and multivariate statistics were applied to identify groupings in the data set. The black rhinoceros horn samples were distinctly different from the white rhinoceros horn samples. This demonstrated that seized rhinoceros horn samples can be identified based on their distinct odour profiles. The chemical odour profiling method has great potential as a rapid and non-invasive screening method in order to combat and track illegal trafficking of wildlife parts. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  8. Capture of the White (Square-Lipped) Rhinoceros Ceratotherium Simum Simum (Burchell) with the Use of Drug Immobilization Technique

    Science.gov (United States)

    Harthoorn, A. M.

    1962-01-01

    The capture of the white (square-lipped) rhinoceros ceratotherium simum simum (Burchell) by the drug immobilization method is described. Details are given of the drug doses and the reactions of the twelve animals immobilized. The value of this method of capture is discussed, especially in relation to the preservation of rhinoceros by moving from contested areas. Imagesp204-a PMID:17649392

  9. Radiographic protocol and normal anatomy of the hind feet in the white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Dudley, Robert J; Wood, Simon P; Hutchinson, John R; Weller, Renate

    2015-01-01

    Foot pathology is a common and important health concern in captive rhinoceroses worldwide, but osteopathologies are rarely diagnosed, partly because of a lack of radiographic protocols. Here, we aimed to develop the first radiographic protocol for rhinoceros feet and describe the radiographic anatomy of the white rhinoceros (Ceratotherium simum) hind foot (pes). Computed tomographic images were obtained of nine cadaver pedes from seven different white rhinoceroses and assessed for pathology. A single foot deemed free of pathology was radiographed using a range of different projections and exposures to determine the best protocol. 3D models were produced from the CT images and were displayed with the real radiographs to describe the normal radiographic anatomy of the white rhinoceros pes. An optimal radiographic projection was determined for each bone in the rhinoceros pes focusing on highlighting areas where pathology has been previously described. The projections deemed to be most useful were D60Pr-PlDiO (digit III), D45Pr45M-PlDiLO (digit II), and D40Pr35L-PlDiLO (digit IV). The primary beam was centered 5-7 cm proximal to the cuticle on the digit of interest. Articular surfaces, ridges, grooves, tubercles, processes and fossae were identified. The radiographic protocol we have developed along with the normal radiographic anatomy we have described will allow for more accessible and effective diagnosis of white rhinoceros foot osteopathologies. © 2014 American College of Veterinary Radiology.

  10. Robust forensic matching of confiscated horns to individual poached African rhinoceros.

    Science.gov (United States)

    Harper, Cindy; Ludwig, Anette; Clarke, Amy; Makgopela, Kagiso; Yurchenko, Andrey; Guthrie, Alan; Dobrynin, Pavel; Tamazian, Gaik; Emslie, Richard; van Heerden, Marile; Hofmeyr, Markus; Potter, Roderick; Roets, Johannes; Beytell, Piet; Otiende, Moses; Kariuki, Linus; du Toit, Raoul; Anderson, Natasha; Okori, Joseph; Antonik, Alexey; Koepfli, Klaus-Peter; Thompson, Peter; O'Brien, Stephen J

    2018-01-08

    Black and white rhinoceros (Diceros bicornis and Ceratotherium simum) are iconic African species that are classified by the International Union for the Conservation of Nature (IUCN) as Critically Endangered and Near Threatened (http://www.iucnredlist.org/), respectively [1]. At the end of the 19 th century, Southern white rhinoceros (Ceratotherium simum simum) numbers had declined to fewer than 50 animals in the Hluhluwe-iMfolozi region of the KwaZulu-Natal (KZN) province of South Africa, mainly due to uncontrolled hunting [2,3]. Efforts by the Natal Parks Board facilitated an increase in population to over 20,000 in 2015 through aggressive conservation management [2]. Black rhinoceros (Diceros bicornis) populations declined from several hundred thousand in the early 19 th century to ∼65,000 in 1970 and to ∼2,400 by 1995 [1] with subsequent genetic reduction, also due to hunting, land clearances and later poaching [4]. In South Africa, rhinoceros poaching incidents have increased from 13 in 2007 to 1,215 in 2014 [1]. This has occurred despite strict trade bans on rhinoceros products and strict enforcement in recent years. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Critical care for a hypothermic and hypoglycemic white rhinoceros (Ceratotherium simum simum) calf.

    Science.gov (United States)

    Warren, Joshua D; Aitken-Palmer, Copper; Citino, Scott B

    2014-09-01

    A 3-day-old, 57.3-kg, male white rhinoceros (Ceratotherium simum simum) neonate presented laterally recumbent with comatose mentation, cold extremities, and severe hypothermia, hypoxemia, and hypoglycemia. Critical care support was initiated with aggressive fluid and warming support as well as dextrose and oxygen supplementation. After initial stabilization, additional complications arose in subsequent weeks including intermittent diarrhea, dry skin with loss of epidermal layers, urticaria on the head, and superficial wounds on the face, ears, feet, and penis. There is a lack of critical care information for rhinoceros calves. However, due to similarities to other Perissodactylids, some general guidelines for critical equid neonatal care were implemented. The calf was hand-raised until weaning and is now a subadult white rhinoceros with no abnormalities.

  12. Neosporosis in an aborted southern white rhinoceros (Ceratotherium simum simum) fetus.

    Science.gov (United States)

    Sangster, Cheryl; Bryant, Benn; Campbell-Ward, Michelle; King, Jessica S; Slapeta, Jan

    2010-12-01

    In December 2008, a southern white rhinoceros (ãsimum simum) aborted a 7-mo gestation male fetus. On hematoxylin and eosin-stained sections of fetal tissues, foci of necrosis were noted in the hepatic parenchyma and were associated with low numbers of lymphocytes, plasma cells, and neutrophils. Protozoal zoites were identified within the hepatic lesions and within the cerebellum. Evaluations utilizing immunohistochemistry, polymerase chain reaction, and DNA sequencing identified the protozoan as Neospora caninum. A microsatellite analysis using MS10 marker showed a unique trinucletoide repeat pattern (ACT), (AGA)19 (TGA)8 distinct from all studied N. caninum to date. This is the first report of N. caninum-related abortion of a rhinoceros fetus of any species and the first report of polymerase chain reaction-confirmed N. caninum infection in any rhinoceros.

  13. Streptococcal endocarditis in a captive southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Houszka, Marek; Dzimira, Stanislaw; Krol, Jaroslaw; Kandefer-Gola, Malgorzata; Ciaputa, Rafal; Sobieraj, Leslaw; Podkowik, Magdalena

    2014-09-01

    Postmortem examination of a 43-yr-old male southern white rhinoceros (Ceratotherium simum simum) revealed gross lesions and histopathologic findings consistent with endocarditis. The animal was born in Umfolozi National Park, South Africa, and then it was moved at 2 yr of age to two successive European zoologic collections. For several weeks prior to death, the animal was increasingly recumbent or assuming a dog-sitting position. Postmortem examination revealed cutaneous pressure sores and multiple rough nodular structures on the mitral valve and left ventricular endocardium. Histopathologic examination revealed vegetative endocarditis, myocardial and hepatocellular degeneration, hepatic fibrosis, and chronic nephritis. Bacterial culture from the oral cavity, trachea, lung, skin, and heart isolated beta hemolytic Streptococcus dysgalactiae subsp. equisimilis and Streptococcus ovis. The cause of death was acute cardiopulmonary failure due mainly to endocarditis and moderate myocardial degeneration. Streptococcal infections are not uncommon causes of morbidity and mortality in rhinoceros. This is the first detailed report of streptococcal endocarditis in a rhinoceros.

  14. Black rhinoceros Diceros bicomis capture, transportation and boma management by the Natal Parks Board

    Directory of Open Access Journals (Sweden)

    R.R. Henwood

    1989-10-01

    Full Text Available Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 The procedure used by the Natal Parks Board in the capture of black rhinoceros Diceros bicomis minor (Drummond, 1876 is outlined. It is emphasised that a successful capture operation requires careful planning and should not be attempted by the uninitiated or by parties who have little or no experience. Dosages of drugs are given, the darting and actual capture procedures highlighted, and aspects of transport and practical boma management are described.

  15. ORAL SQUAMOUS CELL CARCINOMA IN AN AGED CAPTIVE WHITE RHINOCEROS (CERATOTHERIUM SIMUM).

    Science.gov (United States)

    Langer, Sandra; Czerwonka, Nadine; Ternes, Kerstin; Herbst, Dr Werner; Koehler, Kernt

    2016-12-01

    A 48-yr-old captive white rhinoceros ( Ceratotherium simum ) was euthanized due to old age, pododermatitis, and progressive laminitis of the middle toe of the left fore- and hindlimbs. Severe chronic necrotizing periodontitis and dental loss was diagnosed, although food intake prior to death had not decreased. In addition, extensive ulceration of the tongue was noted. Histologically, squamous cell carcinoma of the tongue was diagnosed with chronic severe ulcerative glossitis. Metastatic lesions were not detected. Clinicians should monitor the oral health of aging rhinoceros due to the occult nature of these lesions.

  16. A case of metastatic uterine adenocarcinoma in a southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Wilson, Margaret; Hermes, Robert; Bainbridge, John; Bassett, Hugh

    2010-03-01

    A 39-yr-old, acyclic, uniparous, female white rhinoceros with a history of recurrent vaginal bleeding was euthanized following a period of respiratory distress and ill-thrift. The rhinoceros' uterus had previously been evaluated by ultrasound and diffuse endometrial hyperplasia and two benign uterine leiomyomas had been diagnosed. At necropsy examination, a large, infiltrative, metastatic uterine adenocarcinoma was found multifocally throughout the uterus, scattered within the peritoneal cavity, on the diaphragm, the splenic capsule, the pleural surface of the lung and mesenteric lymph nodes. A large volume (100 L) of ascites fluid was present in the abdominal and pleural cavities.

  17. LATE PLEISTOCENE LAST OCCURRENCES OF THE NARROW-NOSED RHINOCEROS STEPHANORHINUS HEMITOECHUS (MAMMALIA, PERISSODACTYLA IN ITALY

    Directory of Open Access Journals (Sweden)

    LUCA PANDOLFI

    2017-04-01

    Full Text Available Several taxa belonging to the so called megafauna became extinct during the late Quaternary in Eurasia. The extinction chronology of the narrow-nosed rhinoceros, Stephanorhinus hemitoechus, in Europe is still uncertain and only estimated around 45 ka. A systematic revision of several rhinoceros findings reveals that this species occurred in Italy at least untill 41 ka BP, at the onset of the Heinrich Event 4. Climatic fluctuations during MIS 3 and habitat fragmentation probably created an increase of small S. hemitoechus populations in southern Europe which had a tendency to become extinct.

  18. Proposed conservation plan for the black rhinoceros Diceros bicomis in South Africa, the TBVC* states and Namibia

    Directory of Open Access Journals (Sweden)

    P.M. Brooks

    1989-10-01

    Full Text Available Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 The conservation plan for the black rhinoceros presents specific aims and management guidelines for the conservation of the African black rhinoceros Diceros bicomis in the Republic of South Africa, the TBVC states and Namibia. The adoption of this plan, and the application of the strategies described therein (managing existing populations, establishing new populations and aspects of captive breeding by the relevant conservation authorities should enhance the survival prospects of this species, both in the region and globally.

  19. The candelabra tree (Euphorbia ingens: a source of water for black rhinoceros in Liwonde National Park, Malawi

    Directory of Open Access Journals (Sweden)

    C.O. Dudley

    1997-08-01

    Full Text Available The introduction of two rhinoceros into a 15 km2 fenced sanctuary within Liwonde National Park provided an opportunity for close observation of the animals feeding on the toxic succulent Euphorbia ingens. Feeding invariably caused the death of the plant and for plants with basal diameters between 5-17 cm, mortality ranged from 40-90 . During the severe drought of 1994 rhinoceros did not drink from the artificial waterhole from approximately 15 July to at least 23 September. As this waterhole was the only source of free water it is believed that the rhinoceros obtained their water from browsing on E. ingens.

  20. EVALUATION OF SERUM FERRITIN AND SERUM IRON IN FREE-RANGING BLACK RHINOCEROS (DICEROS BICORNIS) AS A TOOL TO UNDERSTAND FACTORS AFFECTING IRON-OVERLOAD DISORDER.

    Science.gov (United States)

    Miller, Michele; Chavey, Patricia Sue; Hofmeyr, Jennifer; Mathebula, Nomkhosi; Doering, Alyssa; Buss, Peter; Olea-Popelka, Francisco

    2016-09-01

    Iron overload disorder (IOD) is a significant health issue for captive black rhinoceros ( Diceros bicornis ). Measurement of serum ferritin with a validated rhinoceros ferritin ELISA has been used extensively to detect animals in U.S. zoos that are at risk of developing IOD. However, there is limited information on serum ferritin levels in free-ranging black rhinoceros using this same assay. Serum ferritin, iron, and gamma-glutamyl transpeptidase (GGT) were determined in 194 black rhinoceros from southern Africa. Mean ferritin in free-ranging black rhinoceros (290.54 ±247.4 ng/ml) was significantly higher than in free-ranging white rhinoceros (64.0 ± 102.4 ng/ml) sampled in this study from Kruger National Park, South Africa. However, there were no significant differences between genders or age groups. Ferritin values varied with geographical location of the black rhinoceros, although this was not clinically significant. Serum iron values were also higher in black rhinoceros (40.4 ± 19.1 μmol/L) compared to white rhinoceros (29.7 ± 10.7 μmol/L). There was no association between ferritin and GGT. This study provides serum ferritin, iron, and GGT values from free-ranging black rhinoceros that can be used for as comparative target values for captive animals.

  1. Anthropogenic influences on conservation values of white rhinoceros.

    Science.gov (United States)

    Ferreira, Sam M; Botha, Judith M; Emmett, Megan C

    2012-01-01

    White rhinoceros (rhinos) is a keystone conservation species and also provides revenue for protection agencies. Restoring or mimicking the outcomes of impeded ecological processes allows reconciliation of biodiversity and financial objectives. We evaluate the consequences of white rhino management removal, and in recent times, poaching, on population persistence, regional conservation outcomes and opportunities for revenue generation. In Kruger National Park, white rhinos increased from 1998 to 2008. Since then the population may vary non-directionally. In 2010, we estimated 10,621 (95% CI: 8,767-12,682) white rhinos using three different population estimation methods. The desired management effect of a varying population was detectable after 2008. Age and sex structures in sink areas (focal rhino capture areas) were different from elsewhere. This comes from relatively more sub-adults being removed by managers than what the standing age distribution defined. Poachers in turn focused on more adults in 2011. Although the effect of poaching was not detectable at the population level given the confidence intervals of estimates, managers accommodated expected poaching annually and adapted management removals. The present poaching trend predicts that 432 white rhinos may be poached in Kruger during 2012. The white rhino management model mimicking outcomes of impeded ecological processes predicts 397 rhino management removals are required. At present poachers may be doing "management removals," but conservationists have no opportunity left to contribute to regional rhino conservation strategies or generate revenue through white rhino sales. In addition, continued trends in poaching predict detectable white rhino declines in Kruger National Park by 2016. Our results suggest that conservationists need innovative approaches that reduce financial incentives to curb the threats that poaching poses to several conservation values of natural resources such as white rhinos.

  2. Anthropogenic influences on conservation values of white rhinoceros.

    Directory of Open Access Journals (Sweden)

    Sam M Ferreira

    Full Text Available White rhinoceros (rhinos is a keystone conservation species and also provides revenue for protection agencies. Restoring or mimicking the outcomes of impeded ecological processes allows reconciliation of biodiversity and financial objectives. We evaluate the consequences of white rhino management removal, and in recent times, poaching, on population persistence, regional conservation outcomes and opportunities for revenue generation. In Kruger National Park, white rhinos increased from 1998 to 2008. Since then the population may vary non-directionally. In 2010, we estimated 10,621 (95% CI: 8,767-12,682 white rhinos using three different population estimation methods. The desired management effect of a varying population was detectable after 2008. Age and sex structures in sink areas (focal rhino capture areas were different from elsewhere. This comes from relatively more sub-adults being removed by managers than what the standing age distribution defined. Poachers in turn focused on more adults in 2011. Although the effect of poaching was not detectable at the population level given the confidence intervals of estimates, managers accommodated expected poaching annually and adapted management removals. The present poaching trend predicts that 432 white rhinos may be poached in Kruger during 2012. The white rhino management model mimicking outcomes of impeded ecological processes predicts 397 rhino management removals are required. At present poachers may be doing "management removals," but conservationists have no opportunity left to contribute to regional rhino conservation strategies or generate revenue through white rhino sales. In addition, continued trends in poaching predict detectable white rhino declines in Kruger National Park by 2016. Our results suggest that conservationists need innovative approaches that reduce financial incentives to curb the threats that poaching poses to several conservation values of natural resources such as

  3. Substantial molecular variation and low genetic structure in Kenya’s black rhinoceros: implications for conservation

    NARCIS (Netherlands)

    Muya, S.M.; Bruford, M.W.; Muigai, A.W.T.; Osiemo, Z.B.; Mwachiro, E.; Ouma, B.O.; Goossens, B.

    2011-01-01

    Kenya’s black rhinoceros population declined by more than 98% from 20,000 individuals in the 1970s to around 400 individuals in 1990 due to the effects of poaching, at which time the surviving individuals were isolated in a series of demographically inviable subpopulations. An initial management

  4. Ovarian superstimulation, transrectal ultrasound-guided oocyte recovery, and IVF in rhinoceros.

    Science.gov (United States)

    Hermes, R; Göritz, F; Portas, T J; Bryant, B R; Kelly, J M; Maclellan, L J; Keeley, T; Schwarzenberger, F; Walzer, C; Schnorrenberg, A; Spindler, R E; Saragusty, J; Kaandorp, S; Hildebrandt, T B

    2009-10-15

    Numerous reports on reproductive pathology in all rhinoceros species illustrate the abundance of female infertility in captive populations. In infertile rhinoceroses, oocyte collection and embryo production could represent the best remaining option for these animals to reproduce and to contribute to the genetic pool. We report here on superstimulation, repeated oocyte recovery, and attempted in vitro fertilization (IVF) in white and black rhinoceroses. Four anestrous rhinoceroses (two white, two black) with unknown follicular status were treated with gonadotropin-releasing hormone analogue, deslorelin acetate, for 6 to 7 d. Number and size of follicles in superstimulated females was significantly higher and larger compared with those in nonstimulated anestrous females (n=9). Ovum pick-up was achieved by transrectal ultrasound-guided follicle aspiration. Up to 15 follicles were aspirated per ovary. During six ovum pick-ups, a total of 29 cumulus-oocyte complexes (COCs) were harvested with a range of 2 to 9 COCs per collection. No postsurgical complications were noted on the rhinoceros ovaries using this minimally invasive approach. Various in vitro maturation (IVM) and IVF protocols were tested on the collected COCs. Despite the low total number of COCs available for IVM and IVF in this study, we can report the first rhinoceros embryo ever produced in vitro. The production of a 4-cell embryo demonstrated the potential of transrectal ultrasound-guided oocyte recovery as a valuable tool for in vitro production of rhinoceros embryos from otherwise infertile females.

  5. First successful artificial insemination with frozen-thawed semen in rhinoceros.

    Science.gov (United States)

    Hermes, R; Göritz, F; Saragusty, J; Sós, E; Molnar, V; Reid, C E; Schwarzenberger, F; Hildebrandt, T B

    2009-02-01

    The first successful artificial insemination (AI) in a rhinoceros was reported in 2007 using fresh semen. Following that success, we decided to evaluate the possibility of using frozen-thawed semen for artificial insemination. Semen, collected from a 35-36 year old Southern white rhinoceros (Ceratotherium simum simum) in the UK was frozen using the directional freezing technique. This frozen semen was used in two intrauterine AI attempts on a 30 years old female rhinoceros in Hungary. The first attempt, conducted 30 days postpartum with an insemination dose of approximately 135 x 10(6) motile cells, failed. The second attempt, conducted two estrus cycles later with an insemination dose of approximately 500 x 10(6) motile cells, resulted in pregnancy and the birth of a healthy offspring. This represents the first successful AI using frozen-thawed semen in a rhinoceros, putting it among very few wildlife species in which AI with frozen-thawed semen resulted in a live birth. The incorporation of AI with frozen-thawed semen into the assisted reproduction toolbox opens the way to preserve and transport semen between distant individuals in captivity or between wild and captive populations, without the need to transport stressed or potentially disease carrying animals. In addition, cryopreserved spermatozoa, in combination with AI, are useful methods to extend the reproductive lifespan of individuals beyond their biological lifespan and an important tool for managing genetic diversity in these endangered mammals.

  6. Alkaline phosphatase as an indicator of true ejaculation in the rhinoceros.

    Science.gov (United States)

    Roth, T L; Stoops, M A; Robeck, T R; Ball, R L; Wolfe, B A; Finnegan, M V; O'Brien, J K

    2010-12-01

    The objective was to determine if seminal alkaline phosphatase (ALP) can serve as an indicator of true ejaculation in the rhinoceros. Concentrations of ALP activity were determined in seminal fractions collected from African black rhinos (Diceros bicornis), an African white rhino (Ceratotherium simum), and an Indian rhino (Rhinoceros unicornis) during electroejaculation. In addition, seminal fractions collected during penile massage of a Sumatran rhino (Dicerorhinus sumatrensis) were assessed. Correlations between ALP activity and sperm concentration, fraction pH, and fraction osmolality were evaluated in the Indian rhino and black rhino. Concentrations of ALP activity in rhino ejaculate fractions ranged from 0.05). Data were insufficient for establishing meaningful correlation coefficients in the Sumatran rhino and white rhino, but preliminary results were in accordance with findings in the Indian rhino and black rhino. We concluded that ALP was present in rhinoceros semen, likely originated from the epididymides and/or testes, and could serve as a useful tool for assessing the production of ejaculatory versus pre-ejaculatory fluid in the rhinoceros. Copyright © 2010 Elsevier Inc. All rights reserved.

  7. Butorphanol with oxygen insufflation corrects etorphine-induced hypoxaemia in chemically immobilized white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Haw, Anna; Hofmeyr, Markus; Fuller, Andrea; Buss, Peter; Miller, Michele; Fleming, Gregory; Meyer, Leith

    2014-10-15

    Opioid-induced immobilization is associated with severe respiratory depression in the white rhinoceros. We evaluated the efficacy of butorphanol and oxygen insufflation in alleviating opioid-induced respiratory depression in eight boma-managed rhinoceros. Chemical immobilization with etorphine, azaperone and hyaluronidase, as per standard procedure for the white rhinoceros, caused severe respiratory depression with hypoxaemia (PaO2 = 27 ± 7 mmHg [mean ± SD]), hypercapnia (PaCO2 = 82 ± 6 mmHg) and acidosis (pH =7.26 ± 0.02) in the control trial at 5 min. Compared to pre-intervention values, butorphanol administration (without oxygen) improved the PaO2 (60 ± 3 mmHg, F (3,21) =151.9, p white rhinoceros by correcting the opioid-induced hypoxaemia, but did not completely reverse all components of respiratory depression. The efficacy of this intervention in reducing respiratory depression in field-captured animals remains to be determined.

  8. Genomic Analysis of Demographic History and Ecological Niche Modeling in the Endangered Sumatran Rhinoceros Dicerorhinus sumatrensis.

    Science.gov (United States)

    Mays, Herman L; Hung, Chih-Ming; Shaner, Pei-Jen; Denvir, James; Justice, Megan; Yang, Shang-Fang; Roth, Terri L; Oehler, David A; Fan, Jun; Rekulapally, Swanthana; Primerano, Donald A

    2018-01-08

    The vertebrate extinction rate over the past century is approximately 22-100 times greater than background extinction rates [1], and large mammals are particularly at risk [2, 3]. Quaternary megafaunal extinctions have been attributed to climate change [4], overexploitation [5], or a combination of the two [6]. Rhinoceroses (Family: Rhinocerotidae) have a rich fossil history replete with iconic examples of climate-induced extinctions [7], but current pressures threaten to eliminate this group entirely. The Sumatran rhinoceros (Dicerorhinus sumatrensis) is among the most imperiled mammals on earth. The 2011 population was estimated at ≤216 wild individuals [8], and currently the species is extirpated, or nearly so, throughout the majority of its former range [8-12]. Understanding demographic history is important in placing current population status into a broader ecological and evolutionary context. Analysis of the Sumatran rhinoceros genome reveals extreme changes in effective population size throughout the Pleistocene. Population expansion during the early to middle Pleistocene was followed by decline. Ecological niche modeling indicated that changing climate most likely played a role in the decline of the Sumatran rhinoceros, as less suitable habitat on an emergent Sundaland corridor isolated Sumatran rhinoceros populations. By the end of the Pleistocene, the Sundaland corridor was submerged, and populations were fragmented and consequently reduced to low Holocene levels from which they would never recover. Past events denuded the Sumatran rhinoceros of genetic diversity through population decline, fragmentation, or some combination of the two and most likely made the species even more susceptible to later exploitation and habitat loss. VIDEO ABSTRACT. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  9. Factors impacting the success of post-mortem sperm rescue in the rhinoceros.

    Science.gov (United States)

    Roth, T L; Stoops, M A; Robeck, T R; O'Brien, J K

    2016-04-01

    The goal of this study was to identify factors that influenced the ability to successfully rescue sperm post-mortem from rhinoceroses maintained in North American zoos. Factors considered included procedural technicalities, individual rhinoceros characteristics and timing. Gross testicular pathology was noted in 17.4% of males (4/23) but did not impact sperm recovery except in one case of azoospermia (4.3%). Of the males in which sperm recovery was attempted (n=21), 62% yielded quality samples considered adequate for cryopreservation (≥ 30% motility with ≥ 2.0 forward progressive status). A high percentage of males (70.6%; 12/17) from which reproductive tissue was removed an d cooled ≤ 4 h after death yielded quality sperm samples, whereas only 25% (1/4) of males from which tissue was removed>4h after death yielded quality samples. Quality samples were recovered 1-51 h post-mortem from rhinoceroses 8 to 36 years old. Neither type of illness (prolonged or acute), or method of death (euthanasia or natural) affected the ability to harvest quality samples (P > 0.05). The Indian rhinoceros yielded significantly more sperm on average (40 × 10(9)) than the African black rhinoceros (3.6 × 10(9); P white rhinoceros (3.2 × 10(9); P Rhinoceros sperm rescue post-mortem is relatively successful across a wide range of variables, especially when tissues are removed and cooled promptly after death, and should be considered standard practice among zoos. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees in urban Guam.

    Science.gov (United States)

    Mankin, R W; Moore, A

    2010-08-01

    Adult and larval Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae: Dynastinae) were acoustically detected in live and dead palm trees and logs in recently invaded areas of Guam, along with Nasutitermes luzonicus Oshima (Isoptera: Termitidae), and other small, sound-producing invertebrates and invertebrates. The low-frequency, long-duration sound-impulse trains produced by large, active O. rhinoceros and the higher frequency, shorter impulse trains produced by feeding N. luzonicus had distinctive spectral and temporal patterns that facilitated their identification and discrimination from background noise, as well as from roaches, earwigs, and other small sound-producing organisms present in the trees and logs. The distinctiveness of the O. rhinoceros sounds enables current usage of acoustic detection as a tactic in Guam's ongoing O. rhinoceros eradication program.

  11. Transport and boma management techniques for black rhinoceros Diceros bicomis as used in the Etosha/Vaalbos operation

    Directory of Open Access Journals (Sweden)

    J.P. Raath

    1989-10-01

    Full Text Available Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 The translocation of six black rhinoceros from the Etosha National Park (Namibia to the Vaalbos National Park (Republic of South Africa is described. Data are provided on the transporting vehicle, the capture, transport and off-loading procedures followed while aspects of boma management are briefly dealt with.

  12. Monitoring methods and techniques for censusing black rhinoceros Diceros bicomis bicomis in Etosha National Park

    OpenAIRE

    A. Cilliers

    1989-01-01

    Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 Water-hole census techniques as developed in Etosha for monitoring black rhinoceros are described in detail. The systematic recording of animals visiting water-holes at night yielded data on the population size and structure, and frequency of drinking of this species. Population size estimates derived from this method were compared with estimates derived from aerial census te...

  13. Artificial insemination in the anoestrous and the postpartum white rhinoceros using GnRH analogue to induce ovulation.

    Science.gov (United States)

    Hildebrandt, T B; Hermes, R; Walzer, C; Sós, E; Molnar, V; Mezösi, L; Schnorrenberg, A; Silinski, S; Streich, J; Schwarzenberger, F; Göritz, F

    2007-06-01

    The objective of this study was to develop AI and to achieve first time pregnancy in a nulliparous rhinoceros. For this, one 24-year-old irregular cycling female white rhinoceros was selected, which had never been mated. The endocrine function was monitored by faecal and serum pregnane analysis. Ultrasound determined the optimal day for AI by measuring follicle sizes of 2.0, 2.6, 3.0, 3.2 cm on days -6, -4, -1, 0 of the induced oestrous cycle, respectively. AI was performed and ovulation induced when a pre-ovulatory-sized follicle was present using GnRH analogue, deslorelin. Fresh semen was deposited in the uterine horn using a patented AI catheter overcoming the hymeneal membrane and torturous cervical folds non-surgically. Moreover, ultrasound monitoring of the uterine involution and ovarian activity on days 16, 26, 30 postpartum facilitated the induction of and the AI on the first postpartum oestrous in a rhinoceros using GnRH analogue. Two consecutive pregnancies were achieved by AI for the first time in the rhinoceros. Pregnancies were diagnosed by elevated serum and faecal 20-oxo-pregnane concentrations. In addition ultrasound measured biometric parameters of the two foetuses on days 86 and 133 of gestation. Two female calves were born after 490 and 502 days of gestation, yet one calf was stillborn. AI in rhinoceros might now be used as assisted reproduction technology tool to boost critically small captive rhinoceros populations.

  14. Technique for venipuncture of the transverse facial vein in the black rhinoceros (Diceros bicornis).

    Science.gov (United States)

    Schlanser, Justin R; Bohart, George W; Paperd, Deborah W; Wagner, Cynthia; Marquardt, Mark; Harrison, Tara M

    2016-11-01

    Through the use of operant conditioning, the authors developed a technique to facilitate obtaining blood samples from a black rhinoceros diagnosed with idiopathic epilepsy. The technique involved operant conditioning to facilitate venipuncture of the transverse facial vein, at an anatomic landmark on the lateral side of the face ventral to the medial canthus of the eye, and dorsal to the lateral commissure of the mouth. The investigators used standard operant conditioning protocols to train the animal for desensitization to a needle puncture in the facial vein. Blood samples obtained from the facial location were free of excessive hemolysis and allowed for large volumes to be collected. The procedure was well-tolerated by the rhinoceros and could be performed regularly without complication. Zoo Biol. 35:570-573, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  15. Anesthetic management of a white rhinoceros (Ceratotherium simum) undergoing an emergency exploratory celiotomy for colic.

    Science.gov (United States)

    Valverde, Alexander; Crawshaw, Graham J; Cribb, Nicola; Bellei, Maria; Gianotti, Giacomo; Arroyo, Luis; Koenig, Judith; Kummrow, Maya; Costa, Maria Carolina

    2010-05-01

    A 26-year-old male white rhinoceros (Ceratotherium simum), weighing approximately 2000 kg was anesthetized for an exploratory celiotomy. Sedation was achieved with intramuscular butorphanol (0.04 mg kg(-1)) and detomidine (0.025 mg kg(-1)) and induction of anesthesia with intravenous glyceryl guaiacolate (50 g) and three intravenous boluses of ketamine (200 mg, each); the trachea was then intubated and anesthesia maintained with isoflurane in oxygen using a circle breathing system. Positioning in dorsal recumbency for the surgery and later in sternal recumbency for the recovery represented challenges that added to the prolonged anesthesia time and surgical approach to partially correct an impaction. The rhinoceros recovered uneventfully after 10.4 hours of recumbency. Anesthetic management for an exploratory celiotomy with a midline approach is possible in rhinoceroses, although planning and extensive staff support is necessary to adequately position the patient.

  16. Monitoring methods and techniques for censusing black rhinoceros Diceros bicomis bicomis in Etosha National Park

    Directory of Open Access Journals (Sweden)

    A. Cilliers

    1989-10-01

    Full Text Available Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 Water-hole census techniques as developed in Etosha for monitoring black rhinoceros are described in detail. The systematic recording of animals visiting water-holes at night yielded data on the population size and structure, and frequency of drinking of this species. Population size estimates derived from this method were compared with estimates derived from aerial census techniques. It was concluded that censusing at a water-hole accounted for 32 percent more animals than a total aerial count by helicopter, and 70 percent more animals than by using a fixed- wing aircraft.

  17. Will current conservation responses save the Critically Endangered Sumatran rhinoceros Dicerorhinus sumatrensis?

    DEFF Research Database (Denmark)

    Havmøller, Rasmus Gren; Payne, Junaidi; Ramono, Widodo

    2016-01-01

    The Critically Endangered Sumatran rhinoceros Dicerorhinus sumatrensis formerly ranged across South-east Asia. Hunting and habitat loss have made it one of the rarest large mammals and the species faces extinction despite decades of conservation efforts. The number of individuals remaining...... is unknown as a consequence of inadequate methods and lack of funds for the intensive field work required to estimate the population size of this rare and solitary species. However, all information indicates that numbers are low and declining. A few individuals persist in Borneo, and three tiny populations...... increased but the species has continued to go locally extinct across its range. Conventional captive breeding has also proven difficult; from a total of 45 Sumatran rhinoceros taken from the wild since 1984 there were no captive births until 2001. Since then only two pairs have been actively bred...

  18. Remotely piloted aircraft systems as a rhinoceros anti-poaching tool in Africa.

    Science.gov (United States)

    Mulero-Pázmány, Margarita; Stolper, Roel; van Essen, L D; Negro, Juan J; Sassen, Tyrell

    2014-01-01

    Over the last years there has been a massive increase in rhinoceros poaching incidents, with more than two individuals killed per day in South Africa in the first months of 2013. Immediate actions are needed to preserve current populations and the agents involved in their protection are demanding new technologies to increase their efficiency in the field. We assessed the use of remotely piloted aircraft systems (RPAS) to monitor for poaching activities. We performed 20 flights with 3 types of cameras: visual photo, HD video and thermal video, to test the ability of the systems to detect (a) rhinoceros, (b) people acting as poachers and (c) to do fence surveillance. The study area consisted of several large game farms in KwaZulu-Natal province, South Africa. The targets were better detected at the lowest altitudes, but to operate the plane safely and in a discreet way, altitudes between 100 and 180 m were the most convenient. Open areas facilitated target detection, while forest habitats complicated it. Detectability using visual cameras was higher at morning and midday, but the thermal camera provided the best images in the morning and at night. Considering not only the technical capabilities of the systems but also the poacherś modus operandi and the current control methods, we propose RPAS usage as a tool for surveillance of sensitive areas, for supporting field anti-poaching operations, as a deterrent tool for poachers and as a complementary method for rhinoceros ecology research. Here, we demonstrate that low cost RPAS can be useful for rhinoceros stakeholders for field control procedures. There are, however, important practical limitations that should be considered for their successful and realistic integration in the anti-poaching battle.

  19. Remotely piloted aircraft systems as a rhinoceros anti-poaching tool in Africa.

    Directory of Open Access Journals (Sweden)

    Margarita Mulero-Pázmány

    Full Text Available Over the last years there has been a massive increase in rhinoceros poaching incidents, with more than two individuals killed per day in South Africa in the first months of 2013. Immediate actions are needed to preserve current populations and the agents involved in their protection are demanding new technologies to increase their efficiency in the field. We assessed the use of remotely piloted aircraft systems (RPAS to monitor for poaching activities. We performed 20 flights with 3 types of cameras: visual photo, HD video and thermal video, to test the ability of the systems to detect (a rhinoceros, (b people acting as poachers and (c to do fence surveillance. The study area consisted of several large game farms in KwaZulu-Natal province, South Africa. The targets were better detected at the lowest altitudes, but to operate the plane safely and in a discreet way, altitudes between 100 and 180 m were the most convenient. Open areas facilitated target detection, while forest habitats complicated it. Detectability using visual cameras was higher at morning and midday, but the thermal camera provided the best images in the morning and at night. Considering not only the technical capabilities of the systems but also the poacherś modus operandi and the current control methods, we propose RPAS usage as a tool for surveillance of sensitive areas, for supporting field anti-poaching operations, as a deterrent tool for poachers and as a complementary method for rhinoceros ecology research. Here, we demonstrate that low cost RPAS can be useful for rhinoceros stakeholders for field control procedures. There are, however, important practical limitations that should be considered for their successful and realistic integration in the anti-poaching battle.

  20. The Sixth Rhino: A Taxonomic Re-Assessment of the Critically Endangered Northern White Rhinoceros

    OpenAIRE

    Groves, Colin P.; Fernando, Prithiviraj; Robovsk?, Jan

    2010-01-01

    BACKGROUND: The two forms of white rhinoceros; northern and southern, have had contrasting conservation histories. The Northern form, once fairly numerous is now critically endangered, while the southern form has recovered from a few individuals to a population of a few thousand. Since their last taxonomic assessment over three decades ago, new material and analytical techniques have become available, necessitating a review of available information and re-assessment of the taxonomy. RESULTS: ...

  1. TINGKAT KESESUAIAN SUAKA MARGASATWA CIKEPUH SEBAGAI HABITAT KEDUA BADAK JAWA (Rhinoceros sondaicus Desmarest, 1822)

    OpenAIRE

    Ribai .; Hadi S Alikodra; Burhanuddin Masyud; U Mamat Rahmat

    2016-01-01

    Javan rhino (Rhinoceros sondaicus) is one of the rarest species in the world so categorized as critically endangered by IUCN. Survival of the rhino in Ujung Kulon National Park is threatened by a variety of factors that could cause these extinct animals, such as: invasion langkap, competition with the bulls, and inbreeding. The strategy should be promoted in maintaining and developing population that is making a second habitat. The purpose of this research is to know the suitability level of ...

  2. Captive breeding of the white rhinoceros, Ceratotherium simum, and the Cape buffalo, Syncerus caffer.

    Science.gov (United States)

    Skinner, J D; Dott, H M; Matthee, A; Hunt, L

    2006-09-01

    Breeding records of 40 white rhinoceros and 155 Cape buffalo were analysed. Three rhinoceros cows bred in captivity, themselves conceived for the first time at 84, 87 and 95 months of age, respectively. Rhinoceros cows breed throughout the year. There is no evidence of a relationship between calving interval and month of birth. Calving intervals were normally distributed about the mean of 34 months and there were no significant differences between bulls, cows or sex of calf. There was no difference in the sex ratio of calves born to young cows nor older cows. The male:female ratio of the calves was 1:1. Younger cows did not have shorter birth intervals. Although captive Cape buffaloes breed throughout the year, there is a preponderance of births in midsummer. There was some evidence that larger cows produce heavier calves and that season of birth may influence birth weight. Male calves weighed 41.20 +/- 0.68 kg vs 39.00 +/- 0.73 kg (range 24-60 kg) for female calves but this difference was not significant. Calving intervals were normally distributed about the mean of 395 days and the male:female ratio of the calves was 1:1.2.

  3. Biochemical values in free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.

    Science.gov (United States)

    Mathebula, Nomkhosi; Miller, Michele; Buss, Peter; Joubert, Jennifer; Martin, Laura; Kruger, Marius; Hofmeyr, Markus; Olea-Popelka, Francisco

    2012-09-01

    Biochemical panels were analyzed on 181 individual free-ranging white rhinoceros (Ceratotherium simum) from Kruger National Park, South Africa. These animals were immobilized between July 2006 and May 2010 for management purposes. Serum and heparinized plasma samples were analyzed using an in-house chemistry analyser (ABAXIS VetScan2). The objectives of this study were to establish biochemical references ranges for Kruger National Park's population of white rhinoceros; to assess differences in values obtained using sera or plasma; and to assess differences in values between gender and different age categories. Significant differences between plasma and serum values were found in most measured parameters except minerals (calcium and magnesium). Because all animals appeared clinically healthy at the time of blood collection, it is hypothesized that choice of anticoagulant may affect certain parameters. Comparison between age categories and gender also resulted in significant differences in a few measured parameters. Identifying differences are important when establishing baseline reference ranges for wildlife populations to allow accurate monitoring of trends that may change over time. The paucity of data on normal biochemical ranges for free-ranging white rhinoceros demonstrates the value of this study and importance of evaluating potential confounding variables.

  4. Discrimination of familiarity and sex from chemical cues in the dung by wild southern white rhinoceros.

    Science.gov (United States)

    Cinková, Ivana; Policht, Richard

    2015-01-01

    Communication in rhinos is primarily mediated by the vocal and olfactory signals as they have relatively poor eyesight. White rhinos are the most social of all the rhinoceros species, they defecate at common dungheaps and the adult bulls use dung and urine to mark their territory. Chemical communication may therefore be particularly important in the social interactions of white rhinos, and its knowledge could be very helpful in their management and conservation. However, no studies have investigated up until now the olfactory discrimination in any rhinoceros species in the wild. We have experimentally studied the reactions of the wild southern white rhinos (Ceratotherium simum) to the dung of familiar and unfamiliar adult females and adult territorial males. We registered the number of sniffing events, the duration of sniffing and the latency of the vigilance posture from the onset of sniffing. The dung of unfamiliar rhinos was sniffed longer than that of familiar rhinos. The rhinos showed a shorter latency of vigilance posture to the familiar dung of males than that of females. For unfamiliar dung, they displayed a shorter latency of vigilance posture to female than male dung. Our results indicate that the rhinos are able to discriminate the familiarity and sex of conspecifics from the smell of their dung. Olfactory cues could therefore play an important role in the social relationships and spatial organization of the southern white rhinoceros.

  5. Density, Viability Conidia And Symptoms of Metarhizium anisopliae infection on Oryctes rhinoceros larvae

    Science.gov (United States)

    Indriyanti, D. R.; Putri, R. I. P.; Widiyaningrum, P.; Herlina, L.

    2017-04-01

    M. anisopliae is parasitic fungus on insect pests; it is used as a biocontrol agent. M. anisopliae can be propagated on maize or rice substrate. M. anisopliae is currently sold in the form of kaolin powder formulations. Before it is used to check the density, viability and pathogenicity of M. anisopliae. However the problem is the kaolin powder very soft, so it difficult to distinguish between kaolin and conidia. This article gives information on how to calculate conidia density, viability and symptoms of M. anisopliae infection on Oryctes rhinoceros larvae. The study was conducted in the laboratory to determine the density and viability. The pathogenicity testing was done using pots. The Pot is containing soil substrate mixed with M. Anispoliae and ten tails O. Rhinoceros larvae per pot. The results showed that the density of M. anisopliae conidia was 1.81 x 108 conidia mL-1 and the viability was 94% within 24 hours. The larval mortality began to emerge in the 1st week, and all larvae died at the sixth week. The symptom of M. anisopliae infection on Oryctes rhinoceros larvae, there was a black spot on the larval integument. The larvae movements become slow and poor appetite; it will die within 3-7 days. The larvae die hard, and the white hyphae grow on the body surface that turns green.

  6. Captive breeding of the white rhinoceros, Ceratotherium simum, and the Cape buffalo, Syncerus caffer : research communication

    Directory of Open Access Journals (Sweden)

    J.D. Skinner

    2006-09-01

    Full Text Available Breeding records of 40 white rhinoceros and 155 Cape buffalo were analysed. Three rhinoceros cows bred in captivity, themselves conceived for the first time at 84, 87 and 95 months of age, respectively. Rhinoceros cows breed throughout the year. There is no evidence of a relationship between calving interval and month of birth. Calving intervals were normally distributed about the mean of 34 months and there were no significant differences between bulls, cows or sex of calf. There was no difference in the sex ratio of calves born to young cows nor older cows. The male:female ratio of the calves was Younger cows did not have shorter birth intervals. Although captive Cape buffaloes breed throughout the year, there is a preponderance of births in midsummer. There was some evidence that larger cows produce heavier calves and that season of birth may influence birth weight. Male calves weighed 41.20 + 0.68 kg vs 39.00 + 0.73 kg (range 24-60 kg for female calves but this difference was not significant. Calving intervals were normally distributed about the mean of 395 days and the male:female ratio of the calves was 1:1.2.

  7. Will tree euphorbias (Euphorbia tetragona and Euphorbia triangularis) survive under the impact of black rhinoceros (Bicornis diceros minor) browsing in the Great Fish River Reserve, South Africa?

    NARCIS (Netherlands)

    Heilmann, L.C.; Jong, de K.; Lent, P.C.; Boer, de W.F.

    2006-01-01

    The impact of black rhinoceros (Bicornis diceros minor) on the tree euphorbias Euphorbia tetragona and Euphorbia triangularis was studied in the Great Fish River Reserve, South Africa. Black rhinoceros pushed over about 5¿7% of the trees in a 2-month period. There was a preference of rhinos for

  8. Impact of black rhinoceros (Diceros bicornis minor) on a local population of Euphorbia bothae in the Great Fish River Reserve, South Africa

    NARCIS (Netherlands)

    Luske, B.L.; Mertens, T.; Lent, P.C.; Boer, de W.F.; Prins, H.H.T.

    2009-01-01

    In the Great Fish River Reserve, South Africa, black rhinoceros (Diceros bicornis minor) feed extensively on a local population of Euphorbia bothae. Maintaining the endangered black rhinoceros and the protected E. bothae population are both conservation priorities of the reserve. Therefore, the

  9. An analysis of the habitat of the Greater One-horned Rhinoceros Rhinoceros unicorns (Mammalia: Perissodactyla: Rhinocerotidae at the Chitwan National Park, Nepal

    Directory of Open Access Journals (Sweden)

    V. Thapa

    2014-09-01

    Full Text Available We used Geographic Information Systems (GIS and landscape-level data obtained from remote sensing sources to build a habitat suitability index model (HSI for the Greater One-horned Rhinoceros Rhinoceros unicornis. The model was based primarily on important habitat requisites of the modeled species, especially food and cover. We extracted food and cover from land cover map and ran focal statistics to determine their proportion in a neighborhood of 70x70 pixels that accounts for the animal’s average mean annual home range, which is ~4km2 = 400 ha = 70x70 pixels x 900 m2 = 4410000/10000 = 441 ha. We used two arbitrarily selected parameters a and Tc to observe the impacts of food and cover on the HSI. We performed sensitivity analysis by varying values of parameters around their nominal, which revealed that the HSI value of a pixel is changed with uncertainty with very low values of a fraction of the food or cover. We identified four habitat types from the HSI map. We used patch and class metrics of FRAGSTATS program to estimate the amount and fragmentation of each habitat type. The metrics provided composition and configuration status for all four habitat types. We found a presence of a total of 476 patches with 517.82km2 belonging to suitable habitat type. These areas can be targeted for management, monitoring and improvement to provide habitat for the target and sympatric species.

  10. Severe hypoxaemia in field-anaesthetised white rhinoceros (Ceratotherium simum and effects of using tracheal insufflation of oxygen

    Directory of Open Access Journals (Sweden)

    M. Bush

    2004-06-01

    Full Text Available White rhinoceros anaesthetised with etorphine and azaperone combination develop adverse physiological changes including hypoxia, hypercapnia, acidosis, tachycardia and hypertension. These changes are more marked in field-anaesthetised rhinoceros. This study was designed to develop a technique to improve safety for field-anaesthetised white rhinoceros by tracheal intubation and oxygen insufflation. Twenty-five free-ranging white rhinoceros were anaesthetised with an etorphine and azaperone combination for translocation or placing microchips in their horns. Once anaesthetised the rhinoceros were monitored prior to crating for transportation or during microchip placement. Physiological measurements included heart and respiratory rate, blood pressure and arterial blood gas samples. Eighteen rhinoceros were intubated using an equine nasogastric tube passed nasally into the trachea and monitored before and after tracheal insufflation with oxygen. Seven rhinoceros were not intubated or insufflated with oxygen and served as controls. All anaesthetised rhinoceros were initially hypoxaemic (percentage arterial haemoglobin oxygen saturation (% O2Sa = 49 % + 16 (mean + SD and PaO2 = 4.666 + 1.200 kPa (35 + 9 mm Hg, hypercapnic (PaCO2 = 8.265 + 1.600 kPa (62 + 12 mm Hg and acidaemic (pHa = 7.171 + 0.073 . Base excess was -6.7 + 3.9 mmol/ℓ, indicating a mild to moderate metabolic acidosis. The rhinoceros were also hypertensive (systolic blood pressure = 21.861 + 5.465 kPa (164 + 41 mm Hg and tachycardic (HR = 107 + 31/min. Following nasal tracheal intubation and insufflation, the % O2Sa and PaO2 increased while blood pHa and PaCO2 remained unchanged.Tracheal intubation via the nose is not difficult, and when oxygen is insufflated, the PaO2 and the % O2Sa increases, markedly improving the safety of anaesthesia, but this technique does not correct the hypercapnoea or acidosis. After regaining their feet following reversal of the anaesthesia, the animals

  11. Severe hypoxaemia in field-anaesthetised white rhinoceros (Ceratotherium simum) and effects of using tracheal insufflation of oxygen.

    Science.gov (United States)

    Bush, M; Raath, J P; Grobler, D; Klein, L

    2004-06-01

    White rhinoceros anaesthetised with etorphine and azaperone combination develop adverse physiological changes including hypoxia, hypercapnia, acidosis, tachycardia and hypertension. These changes are more marked in field-anaesthetised rhinoceros. This study was designed to develop a technique to improve safety for field-anaesthetised white rhinoceros by tracheal intubation and oxygen insufflation. Twenty-five free-ranging white rhinoceros were anaesthetised with an etorphine and azaperone combination for translocation or placing microchips in their horns. Once anaesthetised the rhinoceros were monitored prior to crating for transportation or during microchip placement. Physiological measurements included heart and respiratory rate, blood pressure and arterial blood gas samples. Eighteen rhinoceros were intubated using an equine nasogastric tube passed nasally into the trachea and monitored before and after tracheal insufflation with oxygen. Seven rhinoceros were not intubated or insufflated with oxygen and served as controls. All anaesthetised rhinoceros were initially hypoxaemic (percentage arterial haemoglobin oxygen saturation (%O2Sa) = 49% +/- 16 (mean +/- SD) and PaO2 = 4.666 +/- 1.200 kPa (35 +/- 9 mm Hg)), hypercapnic (PaCO2 = 8.265 +/- 1.600 kPa (62 +/- 12 mm Hg)) and acidaemic (pHa = 7.171 +/- 0.073 ). Base excess was -6.7 +/- 3.9 mmol/l, indicating a mild to moderate metabolic acidosis. The rhinoceros were also hypertensive (systolic blood pressure = 21.861 +/- 5.465 kPa (164 +/- 41 mm Hg)) and tachycardic (HR = 107 +/- 31/min). Following nasal tracheal intubation and insufflation, the %O2Sa and PaO2 increased while blood pHa and PaCO2 remained unchanged. Tracheal intubation via the nose is not difficult, and when oxygen is insufflated, the PaO2 and the %O2Sa increases, markedly improving the safety of anaesthesia, but this technique does not correct the hypercapnoea or acidosis. After regaining their feet following reversal of the anaesthesia, the

  12. The microbial community in the feces of the white rhinoceros (Ceratotherium simum as determined by barcoded pyrosequencing analysis.

    Directory of Open Access Journals (Sweden)

    Gaorui Bian

    Full Text Available As a non-ruminant herbivore, the white rhinoceros has the ability to utilize fibrous plant matter through microbial fermentation in the hindgut. So far, there has been no report using molecular techniques to study the gut microbiota of the white rhinoceros. We used barcoded pyrosequencing to characterize 105,651 sequences of 16S rRNA genes obtained from fecal samples from five white rhinoceroses. Results showed that Firmicutes and Bacteroidetes were the predominant phyla in the samples, which were comprised largely of unclassified bacteria. The microbiota of one animal treated with drug therapy differed from those in other healthy animals, and was dominated by Aerococcus -related bacteria. The core microbiota in the healthy rhinoceros were dominated by phyla Firmicutes and Bacteroidetes, represented by the Ruminococcaceae, Lachnospiraceae, Rikenellaceae and Prevotellaceae families. The present work provides a phylogenetic framework for understanding the complex microbial community of the rhinoceros; however, further studies are required to link the distinctive microbiota with their digestive role in the hindgut of the white rhinoceros.

  13. The microbial community in the feces of the white rhinoceros (Ceratotherium simum) as determined by barcoded pyrosequencing analysis.

    Science.gov (United States)

    Bian, Gaorui; Ma, Li; Su, Yong; Zhu, Weiyun

    2013-01-01

    As a non-ruminant herbivore, the white rhinoceros has the ability to utilize fibrous plant matter through microbial fermentation in the hindgut. So far, there has been no report using molecular techniques to study the gut microbiota of the white rhinoceros. We used barcoded pyrosequencing to characterize 105,651 sequences of 16S rRNA genes obtained from fecal samples from five white rhinoceroses. Results showed that Firmicutes and Bacteroidetes were the predominant phyla in the samples, which were comprised largely of unclassified bacteria. The microbiota of one animal treated with drug therapy differed from those in other healthy animals, and was dominated by Aerococcus -related bacteria. The core microbiota in the healthy rhinoceros were dominated by phyla Firmicutes and Bacteroidetes, represented by the Ruminococcaceae, Lachnospiraceae, Rikenellaceae and Prevotellaceae families. The present work provides a phylogenetic framework for understanding the complex microbial community of the rhinoceros; however, further studies are required to link the distinctive microbiota with their digestive role in the hindgut of the white rhinoceros.

  14. puff-adder, Bitis arietans

    African Journals Online (AJOL)

    blood cells which fill the lumen of the fIne capillaries related to the base of the epithelial cells of the tubular gland elements. S.·Afr. Tydsh. Dierk. 1983. 18(2). The duct epithelium is cuboidal secretory epithelium with the same characteristics as that lining the main gland. The accessory gland (Figures 3 & 4) is divided into a ...

  15. SOLITARY OSTEOCHONDROMA OF THE DISTAL THIRD METACARPAL BONE IN A TWO-YEAR-OLD WHITE RHINOCEROS (CERATOTHERIUM SIMUM).

    Science.gov (United States)

    Smit, Yolandi; Steyl, Johan; Marais, Johan

    2016-12-01

    A privately owned, 2-yr-old, 600-kg, intact male white rhinoceros presented with a progressive lameness of the left front limb of 8-mo duration with a focal swelling situated over the dorsoproximal aspect of third metacarpophalangeal joint. Radiographs of the affected limb showed a 28 mm × 26 mm exostosis at the dorsodistal third metacarpal bone. Surgical removal of the exostosis was performed and histopathologic examination confirmed a solitary osteochondroma. A follow-up examination 1 yr after surgical removal revealed total abolishment of the left front limb lameness. This report documents the first diagnosis and treatment of solitary osteochondroma in a white rhinoceros.

  16. Digestion coefficients achieved by the black rhinoceros (Diceros bicornis), a large browsing hindgut fermenter.

    Science.gov (United States)

    Clauss, M; Castell, J C; Kienzle, E; Dierenfeld, E S; Flach, E J; Behlert, O; Ortmann, S; Streich, W J; Hummel, J; Hatt, J-M

    2006-08-01

    In contrast to the grazing white (C. simum) and Indian (R. unicornis) rhinoceros, the black rhinoceros (D. bicornis) is an exclusive browser. Due to the particular fermentation characteristics of browse, one would expect browsers to display both shorter ingesta retention times and lower digestion coefficients on comparable diets than grazers. In order to generate a database to test this hypothesis, we performed digestibility studies in eight black rhinoceroses (D. bicornis) from three zoological institutions, using total faecal collection for the quantification of faecal output. One to three regularly fed zoo rations of roughage, concentrates and varying proportions of browse material were used per animal. Additional data was taken from three hitherto unpublished studies as well as several published sources. When compared with horses on similar rations, black rhinoceroses achieved lower digestion coefficients for organic matter and CF. In general, an increase in dietary CF content led to a steeper decrease in organic matter and GE digestibility in black rhinoceroses than in horses. When comparing available data for rhinoceroses, browsing species showed a steeper decrease in organic matter digestibility than grazing species with increasing dietary cell wall content. Endogenous losses as determined by linear regression analysis were within the range reported for horses and Indian rhinoceroses. The results suggest that the horse is not a useful model animal for evaluating diets for black rhinoceroses energetically. In general, diets fed to captive black rhinoceroses seem to include higher proportions of concentrates than diets for other rhinoceros species, and an increase in browse or roughage would reduce digestion coefficients to levels observed in animals fed natural forage.

  17. Law enforcement pertaining to illicit trafficking in rhinoceros horn and other trophies

    Directory of Open Access Journals (Sweden)

    J. Wright

    1989-10-01

    Full Text Available Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 The effective police control of illegal traffic in game trophies, as well as the procedures used by law enforcement officers in their investigations are discussed. It is also very relevant to wildlife officers dealing with law enforcement to be aware of, and to understand the statutory restraints placed on their methods of investigation by law. Existing law enforcement bodies on the international scene are mentioned.

  18. Absence of hypomethylation and LINE-1 amplification in a white x black rhinoceros hybrid.

    Science.gov (United States)

    Dobigny, Gauthier; Waters, Paul D; Robinson, Terence J

    2006-05-01

    Genomic stress resulting from the interspecific hybridization of marsupials has been shown to lead to hypomethylation and transposable element over-amplification. Here we investigated both methylation status and transposable element (LINE-1) activity in an F1 hybrid between the black (Diceros bicornis) and white rhinoceros (Ceratotherium simum). Our data show that in this instance the hybrid genome was not characterised by gross hypomethylation and LINE-1 over-amplification thus extending previous investigations on eutherian mammals. These findings underscore observations that wide-scale genomic instability involving hypomethylation and mobile element release may be marsupial specific phenomena within Mammalia.

  19. Schistosomus reflexus-like malformation in a southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Lankton, Julia S; Vanderhart, Daniel J; Terrell, Scott P

    2014-09-01

    A 7.5-yr-old female southern white rhinoceros (Ceratotherium simum simum) aborted spontaneously at approximately 13 mo gestation. The fetus exhibited malformations consistent with schistosomus reflexus syndrome, including spinal inversion and ventral midline abdominal defect with externalization of abdominal viscera. A computed tomography was performed and revealed severe scoliosis, a spiral rotation of the spine, multiple vertebral anomalies, hypoplasia of multiple bones of the skull, and mild prognathism. This is the first report of schistosomus reflexus-like syndrome in a nondomestic species, and the first report to characterize these skeletal changes by three-dimensional computed tomography.

  20. Medical management of a patent urachus in a southern white rhinoceros (Ceratotherium simum simum) calf.

    Science.gov (United States)

    Bloch, Rebecca A; Haefele, Holly; Stephens, Lisa

    2014-06-01

    A female southern white rhinoceros (Ceratotherium simum simum) calf presented with a patent urachus at 5 days of age. It was immobilized for examination and medical treatment was elected over surgical repair. Systemic antibiotics and topical treatment of the site by cleaning with chlorhexidine solution were followed by silver nitrate cautery. This animal required no further treatment and has reached 1 yr of age with no other medical problems. Although patent urachus is relatively common in domestic animals, especially horses, neither the problem nor its resolution are well documented in exotic animals and surgical intervention is more commonly performed.

  1. Elaborate horns in a giant rhinoceros beetle incur negligible aerodynamic costs.

    Science.gov (United States)

    McCullough, Erin L; Tobalske, Bret W

    2013-05-07

    Sexually selected ornaments and weapons are among nature's most extravagant morphologies. Both ornaments and weapons improve a male's reproductive success; yet, unlike ornaments that need only attract females, weapons must be robust and functional structures because they are frequently tested during male-male combat. Consequently, weapons are expected to be particularly costly to bear. Here, we tested the aerodynamic costs of horns in the giant rhinoceros beetle, Trypoxylus dichotomus. We predicted that the long, forked head horn would have three main effects on flight performance: increased body mass, an anterior shift in the centre of mass and increased body drag. We found that the horns were surprisingly lightweight, and therefore had a trivial effect on the male beetles' total mass and mass distribution. Furthermore, because beetles typically fly at slow speeds and high body angles, horns had little effect on total body drag. Together, the weight and the drag of horns increased the overall force required to fly by less than 3 per cent, even in the largest males. Because low-cost structures are expected to be highly evolutionarily labile, the fact that horns incur very minor flight costs may have permitted both the elaboration and diversification of rhinoceros beetle horns.

  2. Successful treatment of a squamous cell carcinoma in a white rhinoceros, Ceratotherium simum.

    Science.gov (United States)

    Goodman, Gidona; Rhind, Susan; Meredith, Anna

    2007-12-01

    A captive 33-year-old male white rhinoceros with seasonal dermatitis was diagnosed with a malignant squamous cell carcinoma on the right flank. Staphylococcus aureus was cultured from the skin lesions. No fungal or yeast was isolated. The dermatitis was treated with a combination of oral antibiotics (trimethoprim-sulphadiazine) and topically with weekly chlorhexidine washes and a mixture of a zinc oxide, balsam peru and bismuth oxide cream. Under azaperone and butorphanol anaesthesia, the skin tumour was surgically removed. The tumour was excised with wide margins and allowed to heal by secondary intention as primary wound closure was not possible. A post-mortem performed 2 years later for an unrelated condition revealed no metastases or recurrence of the skin tumour. It was presumed that chronic irritation or trauma may have contributed to the development of the skin tumour. This is the first detailed report of the successful treatment of a squamous cell carcinoma not associated with the horn in a rhinoceros.

  3. EVALUATION OF CARDIORESPIRATORY, BLOOD GAS, AND LACTATE VALUES DURING EXTENDED IMMOBILIZATION OF WHITE RHINOCEROS (CERATOTHERIUM SIMUM).

    Science.gov (United States)

    Buss, Peter; Olea-Popelka, Francisco; Meyer, Leith; Hofmeyr, Jennifer; Mathebula, Nomkhosi; Kruger, Marius; Brüns, Angela; Martin, Laura; Miller, Michele

    2015-06-01

    Ten white rhinoceros (Ceratotherium simum) were immobilized for a total of 13 procedures in holding facilities in Kruger National Park using etorphine, azaperone, and hyaluronidase to assess the effect of extended immobilization on serial cardiorespiratory, blood gas, and lactate values. Butorphanol was administered intravenously following initial blood collection and physiologic assessment (t=0). Respiratory and cardiovascular parameters, body temperature, and arterial blood gases were monitored at 10-min intervals for a total of 100 min. Initial parameters at the time of recumbency revealed severe hypoxemia, hypercapnia, tachycardia, an increased alveolar-arterial (A-a) gradient, and mildly elevated lactate levels. At 10 min and 20 min, there were significant (Prhinoceros remained severely hypoxemic for the remainder of the procedure (median PaO2=50.5 mm Hg, 95% confidence interval, 43.8-58.1). Median values for respiratory rate (5 breaths/min) and arterial partial pressure of carbon dioxide (PaCO2; 68.5 mm Hg) did not change significantly for the remaining 80 min. Median lactate, base excess, bicarbonate, and pH values improved between 20 and 100 min despite the persistent hypercapnia, indicating that the animals adequately compensated for respiratory and lactic acidosis. White rhinoceros were immobilized for 100 min with no negative effects, a desirable outcome if procedures require extended chemical immobilization without oxygen supplementation.

  4. Sex and species recognition by wild male southern white rhinoceros using contact pant calls.

    Science.gov (United States)

    Cinková, Ivana; Policht, Richard

    2016-03-01

    Recognition of information from acoustic signals is crucial in many animals, and individuals are under selection pressure to discriminate between the signals of conspecifics and heterospecifics or males and females. Here, we first report that rhinos use information encoded in their calls to assess conspecifics and individuals of closely related species. The southern (Ceratotherium simum) and critically endangered northern (C. cottoni) white rhinos are the most social out of all the rhinoceros species and use a contact call pant. We found that southern white rhino pant calls provide reliable information about the caller's sex, age class and social situation. Playback experiments on wild territorial southern white rhinoceros males revealed that they responded more strongly to the pant calls of conspecific females compared to the calls of other territorial males. This suggests that pant calls are more important form of communication between males and females than between territorial males. Territorial southern males also discriminated between female and territorial male calls of northern species and reacted more intensively to the calls of northern than southern males. This might be caused by a novelty effect since both species naturally live in allopatry. We conclude that white rhinos can directly benefit from assessing individuals at long distances using vocal cues especially because their eyesight is poor. Pant calls thus likely play a significant role in their social relationships and spatial organization. In addition, better understanding of vocal communication in white rhinos might be helpful in conservation management particularly because of their low reproduction in captivity.

  5. Habitat Suitability Modeling of Javan Rhino (Rhinoceros sondaicus Desmarest 1822 Ujung Kulon National Park

    Directory of Open Access Journals (Sweden)

    U Mamat Rahmat

    2012-08-01

    Full Text Available Javan rhino (Rhinoceros sondaicus Desmarest 1822 is the rarest species among 5 species of rhinos in the world. Its distribution is currently limited only in Ujung Kulon National Park (UKNP, Indonesia.  Due to the small population size and its limited distribution, it is listed as critically endangered species in the IUCN Redlist. The main objectives of this research were: 1 to identify the dominant habitat components that determine the presence of javan rhino in UKNP; 2 to analyze habitat suitability of javan rhino (Rhinoceros sondaicus Desmarest 1822 in UKNP using geographical information system; and 3 to examine its habitat condition. Results of the research found eight components identified to be crucial for Javan rhino habitat and appropriate for spatial modelling of habitat suitability, of which four were the most dominant factors influencing the frequency of javan rhino presence. These four dominants factors were slope, distance from wallow sites, distance from rumpang (open area, and distance from beach (pKeywords:  Javan rhino, habitat suitability, preference, spatial modelling

  6. Activation of southern white rhinoceros (Ceratotherium simum simum) estrogen receptors by phytoestrogens and their potential role in thereproductive failure of captive-born females

    Science.gov (United States)

    The captive southern white rhinoceros (SWR; Ceratotherium simum simum) population serves as an important genetic reservoir critical to the conservation of this vulnerable species. Unfortunately, captive populations are declining due to the poor reproductive success of captive-bor...

  7. Transport and boma management techniques for black rhinoceros Diceros bicomis as used in the Etosha/Vaalbos operation

    OpenAIRE

    J.P. Raath; A.J. Hall-Martin

    1989-01-01

    Selected Papers from the Rhinoceros Conservation Workshop, Skukuza, Kruger National Park,31 August – 4 September 1988 The translocation of six black rhinoceros from the Etosha National Park (Namibia) to the Vaalbos National Park (Republic of South Africa) is described. Data are provided on the transporting vehicle, the capture, transport and off-loading procedures followed while aspects of boma management are briefly dealt with.

  8. Infectivity of Oryctes Nudivirus Produced on Cell Culture Dsir Ha-1179 Against Larvae and Its Effects on Feeding of Neonates of Rhinoceros Beetle, Oryctes Rhinoceros

    International Nuclear Information System (INIS)

    Nur Ain Farhah Ros Saidon Khudri; Wahizatul Afzan Azmi; Ramle Moslim; Norman Kamarudin; Siti Ramlah Ahmad Ali

    2016-01-01

    The Oryctes nudivirus (OrNV) is a classical biocontrol agent for a major oil palm insect pest the rhinoceros beetle, Oryctes rhinoceros. The infectivity of three Malaysian indigenous types of OrNV types A, B and C were tested on larvae and neonates. On larvae, the peroral inoculation test technique indicated that the highest mortality of 100 % was achieved using type A produced from cell culture DSIR-HA-1179, while the highest infectivity of 41.7 % was recorded for type A prepared from infected guts. No differences in infectivity were observed on other treatments, which ranged from 13.1 % to 41.7 %. In the substrate contamination inoculation test technique, results showed that the level of infectivity was even lower in all OrNV treatments, ranging as low as 6.7 % to only 15.0 %. Low infectivity was mainly due to inactivation of virus inocula in the larval food substrates. Based on the results for both inoculation methods, the OrNV type C prepared from cell culture DSIR-HA-1179 was found more effective in controlling the L3 larvae than the other types of OrNV. The impact of OrNV infection on food consumption by the neonates was studied. The feeding of inoculated neonates with OrNV reduced rapidly, especially at the early stage of the experiment between eight days after treatment (DAT) to 16 DAT. At this period, the food consumption by all tested OrNV was rapidly reduced and maintained low until the experiment ended at 60 DAT. The highest feeding reduction rate was on neonates treated by type A (-0.074x) followed by neonates treated by type C (-0.053x) and type B (-0.035x). Therefore, it was suggested that besides on highly virulent, the selection of OrNV for field release should also based on high reduction rate on food consumption by the infected insects on plant hosts. (author)

  9. Vitamin D status of wild Ricord's iguanas (Cyclura ricordii) and captive and wild rhinoceros iguanas (Cyclura cornuta cornuta) in the Dominican Republic.

    Science.gov (United States)

    Ramer, Jan C; Maria, Roberto; Reichard, Tim; Tolson, Peter J; Chen, Tai C; Holick, Michael F

    2005-06-01

    Calcidiol (25-hydroxyvitamin D) values are reported for 22 wild Ricord's iguanas (Cyclura ricordii) and seven wild rhinoceros iguanas (Cyclura cornuta cornuta). Calcitriol (1,25-hydroxyvitamin D) values are reported for 12 wild Ricord's iguanas and seven wild rhinoceros iguanas. These animals were captured as part of a larger health assessment study being conducted on Ricord's iguanas in Isla Cabritos National Park, Dominican Republic. A total of 13 captive rhinoceros iguanas held outdoors at Parque Zoológico Nacional were also sampled for comparison. Mean concentrations of 25-hydroxyvitamin D were 554 nmol/L (222 ng/ml) with a range of 250-1,118 nmol/L (100-448 ng/ml) for wild Ricord's iguanas, 332 nmol/L (133 ng/ml) with a range of 260-369 nmol/L (104-148 ng/ml) for wild rhinoceros iguanas, and 317 nmol/L (127 ng/ml) with a range of 220-519 nmol/L (88-208 ng/ml) for captive rhinoceros iguanas. On the basis of these results, serum concentrations of at least 325 nmol/L (130 ng/ml) for 25-hydroxyvitamin D should be considered normal for healthy Ricord's and rhinoceros iguanas.

  10. Cross-species chromosome painting in the Perissodactyla: delimitation of homologous regions in Burchell's zebra (Equus Burchellii) and the white (Ceratotherium Simum) and black rhinoceros (Diceros Bicornis).

    Science.gov (United States)

    Trifonov, V; Yang, F; Ferguson-Smith, M A; Robinson, T J

    2003-01-01

    Conserved chromosomal segments in the black rhinoceros, Diceros Bicornis (DBI, 2n = 84), and its African sister-species the white rhinoceros, Ceratotherium Simum (CSI, 2n = 82), were detected using Burchell's zebra (Equus Burchellii, EBU, 2n = 44) chromosome-specific painting probes supplemented by a subset of those developed for the horse (Equus Caballus, ECA, 2n = 64). In total 41 and 42 conserved autosomal segments were identified in C. Simum and D. Bicornis respectively. Only 21 rearrangements (20 fissions and 1 fusion) are necessary to convert the Burchell's zebra karyotype into that of the white rhinoceros. One fission distinguishes the D. Bicornis and C. Simum karyotypes which, excluding heterochromatic differences, are identical in all respects at this level of resolution. Most Burchell's zebra chromosomes correspond to two rhinoceros chromosomes although in four instances (EBU18, 19, 20 and 21) whole chromosome synteny has been retained among these species. In contrast, one rhinoceros chromosome (DBI1, CSI1) comprises two separate Burchell's zebra chromosomes (EBU11 and EBU17). In spite of the high diploid numbers of the two rhinoceros species their karyotypes are surprisingly conserved offering a glimpse of the putative ancestral perissodactyl condition and a broader understanding of genome organization in mammals. Copyright 2003 S. Karger AG, Basel

  11. EVALUATION OF BLOOD GAS VALUES IN ANESTHETIZED SOUTHERN WHITE RHINOCEROS ( CERATOTHERIUM SIMUM) VENTILATED WITH A NOVEL DEMAND VENTILATOR IN A ZOOLOGICAL PARK SETTING.

    Science.gov (United States)

    Jeon, Mark; Mama, Khursheed R; Zuba, Jeffrey R; Lamberski, Nadine; Oosterhuis, James E; Clancy, Meredith M; Delk, Katie W; Kinney, Matthew E; Morris, Patrick J; Olea-Popelka, Francisco

    2017-12-01

    Rhinoceros conservation efforts are essential to the survival of the species. One such effort is focused on using advanced reproductive technologies to produce viable northern white rhinoceros ( Ceratotherium simum cottoni) embryos for implantation into southern white rhinoceros ( Ceratotherium simum simum) surrogates. Anesthesia may be required to facilitate necessary procedures in these surrogate rhinoceros, but commonly reported side effects including hypercapnia and hypoxemia limit anesthetic recumbency time due to animal safety concerns. Although many interventions have been attempted, success in improving these physiologic parameters to date is mixed. The objective of this report is to describe arterial pH (pHa), blood gas (PaO 2 and PaCO 2 ), bicarbonate, base excess, lactate, and cardiovascular (heart rate, direct arterial blood pressure) values recorded in seven intubated and ventilated female southern white rhinoceros anesthetized for reproductive examinations in a zoological park setting. Anesthetic induction was accomplished using etorphine, medetomidine, butorphanol, and midazolam. The primary hypotheses were that PaO 2 and PaCO 2 would improve after intubation and mechanical ventilation. Induction and recovery observations were also summarized. Physiologic and laboratory data were analyzed using a mixed linear regression model using ranks. Statistical significance was set at P white rhinoceros.

  12. Postinduction butorphanol administration alters oxygen consumption to improve blood gases in etorphine-immobilized white rhinoceros.

    Science.gov (United States)

    Buss, Peter; Miller, Michele; Fuller, Andrea; Haw, Anna; Stout, Eliza; Olea-Popelka, Francisco; Meyer, Leith

    2018-01-01

    To investigate the effects of postinduction butorphanol administration in etorphine-immobilized white rhinoceros on respiration and blood gases. Randomized crossover study. A group of six sub-adult male white rhinoceros. Etorphine, or etorphine followed by butorphanol 12 minutes after recumbency, was administered intramuscularly [2.5 mg etorphine, 25 mg butorphanol (1000-1250 kg), or 3.0 mg etorphine, 30 mg butorphanol (1250-1500 kg)]. Sampling started at 10 minutes after initial recumbency, and was repeated at 5 minute intervals for 25 minutes. Arterial blood gases, limb muscle tremors, expired minute ventilation and respiratory frequency were measured at each sampling point. Calculated values included alveolar-arterial oxygen gradient [ [Formula: see text] ], expected respiratory minute volume (V˙e), tidal volume (Vt), oxygen consumption ( [Formula: see text] ) and carbon dioxide production ( [Formula: see text] ). Etorphine administration resulted in an initial median (range) hypoxaemia [arterial partial pressure of oxygen 25.0 (23.0-28.0) mmHg], hypercapnia [arterial partial pressure of carbon dioxide 76.2 (67.2-81.2) mmHg], increased [Formula: see text] [41.7 (36.6-45.1) mmHg, [Formula: see text] [11.1 (10.0-12.0) L minute -1 ] and muscle tremors. Butorphanol administration was followed by rapid, although moderate, improvements in arterial partial pressure of oxygen [48.5 (42.0-51.0) mmHg] and arterial partial pressure of carbon dioxide [62.8 (57.9-75.2) mmHg]. In rhinoceros administered butorphanol, [Formula: see text] [4.4 (3.6-5.1) L minute -1 ] and [Formula: see text] [4.2 (3.8-4.4) L minute -1 ] were lower than in those not administered butorphanol. Increased arterial oxygen tension was associated with lower oxygen consumption (p=0.002) which was positively associated with lower muscle tremor scores (prhinoceros resulted from an increased [ [Formula: see text] ] and increased [Formula: see text] and [Formula: see text] associated with muscle

  13. How healthy are the rhinoceros populations in the Hluhluwe-iMfolosi Park?

    Directory of Open Access Journals (Sweden)

    LP Fatti

    2013-06-01

    Full Text Available Arising from a study conducted in the Hluhluwe-iMfolosi Park by the author in the late nineties, a method is proposed for improving the estimate of the size of a wildlife population by combining data from current and past surveys. The method is based on a simple state space model which takes into account the (unknown birth rate in the population and all known losses (mortalities and relocations and gains (introductions in the population between successive surveys, as well as the errors in the survey estimates. The method is applied to the White- and Black Rhinoceros populations in the Hluhluwe-iMfolosi Park and tentative conclusions are drawn on the health of these two populations.

  14. Vesiculobullous skin reaction temporally related to firocoxib treatment in a white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Stringer, Elizabeth M; De Voe, Ryan S; Linder, Keith; Troan, Brigid; McCalla-Martin, Amy; Loomis, Michael R

    2012-03-01

    A 40 yr-old female white rhinoceros (Ceratotherium simum) suffered from chronic nail-bed abscesses. Due to worsening of clinical signs, the animal's nonsteroidal anti-inflammatory treatment was switched to firocoxib. Approximately 7 days after this change, the animal developed multifocal vesicles and bullae along the lateral aspects of the thorax and abdomen, the dorsum, and the proximal limbs. Cytology and culture did not identify an infectious etiology. Histologically, the lesions consisted of a severe, subacute vesiculobullous dermatitis with intraepidermal to subepidermal clefting with areas of individual keratinocyte necrosis and minor neutrophilic epidermal infiltrates. These findings are similar to those seen in some drug reactions in people; therefore an adverse drug reaction to the firocoxib was suspected.

  15. Pulmonary infection due to Mycobacterium bovis in a black rhinoceros (Diceros bicornis minor) in South Africa.

    Science.gov (United States)

    Espie, Ian W; Hlokwe, Tiny M; Gey van Pittius, Nicolaas C; Lane, Emily; Tordiffe, Adrian S W; Michel, Anita L; Müller, Annélle; Kotze, Antoinette; van Helden, Paul D

    2009-10-01

    We report a case of tuberculosis due to infection with Mycobacterium bovis in an elderly male black rhinoceros (Diceros bicornis minor) from the Limpopo Province in South Africa. The animal was euthanized due to very poor condition, old age, and dental attrition. Necropsy examination revealed two small nonencapsulated granulomas (approximately 40-mm diameter) in the dorsocaudal lobe of the left lung. Sequencing of isolated crude lung tissue PCR product and boiled lung culture samples confirmed that the causative organism was M. bovis. Genotyping revealed limited similarities with M. bovis strains isolated thus far from South African cattle or wildlife. The source of the infection could not be determined. This case illustrates that M. bovis could impact conservation of free-ranging rare and endangered species. Effective diagnostics are urgently needed for different animal species, such as white or black rhinoceroses, to certify with a reasonable degree of certainty that these animals are free of tuberculosis in natural habitats.

  16. Serosurvey for selected viral agents in white rhinoceros (Ceratotherium simum) in Kruger National Park, 2007.

    Science.gov (United States)

    Miller, Michele; Buss, Peter; Joubert, Jenny; Maseko, Nomkhosi; Hofmeyr, Markus; Gerdes, Truuske

    2011-03-01

    One hundred serum samples collected from free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park (KNP) during the 2007 capture season were selected for measurement of antibody levels to several different vector-borne viral agents. These infectious diseases were chosen to compare with an earlier serosurvey that had been conducted in KNP in rhinos during 1987-1997. Positive antibody titers were found against epizootic hemorrhagic disease (EHD) of deer (8%), Bluetongue (BT) (1%), and Rift Valley fever (RVF) (49%). However, none of the 100 animals tested had detected antibody levels to African horse sickness (AHS). These values were in sharp contrast to those measured in the 1987-1997 survey in KNP white rhinos (AHS 60%, EHD 30%, BT 37%, RVF 0%). Vector-borne viral infection prevalence in white rhinos in the same geographical location appears to vary over time and may be important for monitoring presence of pathogens in an ecosystem.

  17. Body condition scoring system for greater one-horned rhino (Rhinoceros unicornis): Development and application.

    Science.gov (United States)

    Heidegger, Eva M; von Houwald, Friederike; Steck, Beatrice; Clauss, Marcus

    2016-09-01

    Preventing obesity in zoo animals is increasingly recognized as an important husbandry objective. To achieve this goal, body condition scoring (BCS) systems are available for an ever-increasing number of species. Here, we present a BCS for the greater one-horned rhinoceros (Rhinoceros unicornis) based on an evaluation (on a scale from 1 to 5) of seven different body regions, and report resulting scores for 62 animals from 27 facilities, based on digital photographs. In animals above 4 years of age, this BCS correlated with the body mass:shoulder height ratio. Although differences between the sexes for individual regions were noted (with consistently higher scores in males for the neck and shoulder and in parous females for the abdomen), the average BCS of all regions did not differ significantly between males (4.3 ± 0.4) and females (4.1 ± 0.5). Linking the BCS to results of a questionnaire survey and studbook information, there were no differences in BCS between animals with and without foot problems or between parous and non-parous females. In a very limited sample of 11 females, those eight that had been diagnosed with leiomyoma in a previous study had a higher BCS (range 3.9-4.9) than the three that had been diagnosed as leiomyoma-free (range 3.5-3.7). The BCS was correlated to the amount of food offered as estimated from the questionnaire. Adjusting the amounts and the nutritional quality of the diet components is an evident measure to maintain animals at a target BCS (suggested as 3-3.5). Zoo Biol. 35:432-443, 2016. © Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  18. Retinal ganglion cell topography and spatial resolving power in the white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Coimbra, João Paulo; Manger, Paul R

    2017-08-01

    This study sought to determine whether the retinal organization of the white rhinoceros (Ceratotherium simum), a large African herbivore with lips specialized for grazing in open savannahs, relates to its foraging ecology and habitat. Using stereology and retinal wholemounts, we estimated a total of 353,000 retinal ganglion cells. Their density distribution reveals an unusual topographic organization of a temporal (2,000 cells/mm 2 ) and a nasal (1,800 cells/mm 2 ) area embedded within a well-defined horizontal visual streak (800 cells/mm 2 ), which is remarkably similar to the retinal organization in the black rhinoceros. Alpha ganglion cells comprise 3.5% (12,300) of the total population of ganglion cells and show a similar distribution pattern with maximum densities also occurring in the temporal (44 cells/mm 2 ) and nasal (40 cells/mm 2 ) areas. We found higher proportions of alpha cells in the dorsal and ventral retinas. Given their role in the detection of brisk transient stimuli, these higher proportions may facilitate the detection of approaching objects from the front and behind while grazing with the head at 45 °. Using ganglion cell peak density and eye size (29 mm, axial length), we estimated upper limits of spatial resolving power of 7 cycles/deg (temporal area), 6.6 cycles/deg (nasal area), and 4.4 cycles/deg (horizontal streak). The resolution of the temporal area potentially assists with grazing, while the resolution of the streak may be used for panoramic surveillance of the horizon. The nasal area may assist with detection of approaching objects from behind, potentially representing an adaptation compensating for limited neck and head mobility. J. Comp. Neurol., 525:2484-2498, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  19. Social and spatial relationships in captive southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Metrione, Lara C; Penfold, Linda M; Waring, George H

    2007-11-01

    Although critical to the conservation of white rhinoceros, captive breeding has proven challenging because of the poor and irregular reproductive health of many captive rhinos, and social interactions may play a significant role. This research investigated the social and spatial relationships of two captive groups of southern white rhinoceros (Ceratotherium simum simum) by examining the frequency of companion changes, the number of space maintenance vocalizations made per hour by each reproductively mature female, and dominant/subordinate interactions. The observed captive rhinos did not change their companionships during the study. They exhibited space maintenance vocalizations and display greater than once per hour, particularly when feeding. Females housed with four calves on 0.033 km(2) exhibited space maintenance vocalizations more frequently (X+/-SE = 6.19+/-0.199/hr) than females housed with one calf and more space (0.06 km(2), X+/-SE = 0.55+/-0.182/hr) and females housed without calves and more space (0.65 km(2), X+/-SE = 1.90+/-0.086/hr). Wider separation of food piles and of females with young calves is suggested to reduce the interpreted spatial stress. The presence of a large number of rhinos in restricted captive space resulted in the formation of herds with dominance hierarchies that were enforced during competition for food and shade. The most subordinate rhino in each of the herds exhibited unusual behaviors such as dung-kicking and nonestrus urine squirting, and neither has ever reproduced. Suppression of subordinate rhinos might lead to social stress that could negatively impact reproductive success. Zoo Biol 26:487-502, 2007. (c) 2007 Wiley-Liss, Inc.

  20. Profiling patterns of fecal 20-oxopregnane concentrations during ovarian cycles in free-ranging southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    van der Goot, Annemieke Catharina; Martin, Graeme Bruce; Millar, Robert Peter; Paris, Monique Christina Johanna; Ganswindt, Andre

    2015-10-01

    Unlike their wild counterparts, many white rhinoceros females in captivity fail to reproduce successfully such that current captive populations are not self-sustaining. The causes of the problem are poorly understood. Variation in cycle length and long periods of acyclicity are characteristics of the majority of these non-reproducing females in captivity but it is unknown whether these characteristics are a feature of reproductively successful free-ranging females. This study therefore aimed to monitor cyclic activity in a wild population of southern white rhinoceros at Lapalala Wilderness, South Africa, by measuring the concentrations of immunoreactive fecal progestagen metabolites (fPM). Five adult females were tracked twice per week for 20 months and if located a fresh fecal sample was collected. Reproductive events and group structural dynamics were also recorded and subsequently correlated with the fPM data. The baseline concentration of fPM was 0.69±0.20μg/g DW while concentrations during pregnancy were 30-400-fold higher. The females exhibited estrous cycle lengths of 30.6±7.7 days and, based on fPM data, gestation length in one female was 502±3 days. Year-round monitoring showed no clear evidence of seasonality in ovarian activity. During cyclic luteal activity females were often seen in the presence of a dominant bull. One female stopped cycling after removal of the local dominant bull and luteal activity only returned after a new bull was introduced. This suggests that white rhinoceros females in the wild might need external stimuli from a male to ovulate. These findings indicate that the irregular cyclicity reported for white rhinoceros housed in zoos and animal parks may result from conditions in captivity and account for reduced fertility. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Two methods to adapt the human haemoglobin-oxygen dissociation algorithm to the blood of white rhinoceros (Ceratotherium simum) and to determine the accuracy of pulse oximetry.

    Science.gov (United States)

    Haymerle, Agnes; Knauer, Felix; Walzer, Chris

    2016-09-01

    To adapt the algorithm for the calculation of oxygen saturation to the blood characteristics of the white rhinoceros by two different methods and to determine the accuracy of conventional pulse oximetry measurements. Adaptation of two mathematical models of the oxygen dissociation curve (ODC). Twenty-five captive white rhinoceros (Ceratotherium simum), including 12 males and 13 females, aged 6-32 years. During 33 anaesthetic events, 94 arterial blood gas samples with 72 simultaneous pulse oximetry measurements were analysed. The calculation of oxygen saturation was adapted to the characteristics of rhinoceros blood using two different methods. Firstly, a mathematical model developed in 1984 and, secondly, an oxygen status algorithm (OSA) produced by the same developer in 2005 were tested for their applicability for clinical use. When arterial partial pressure of oxygen is >7.98 kPa (60 mmHg), oxygen saturation exceeds 95%. At partial pressures of 6.12-6.52 kPa (46-49 mmHg) Method 1 determined oxygen saturations of 92.5-95.3% and Method 2 oxygen saturations of 90.2-91.6%. Both methods resulted in similar ODCs and accounted for the low p50 value of rhinoceros blood. Method 1 provided better adaptation in respect to the physiological parameters of the rhinoceros, especially with regard to the Bohr effect, than Method 2. Pulse oximetry was an unreliable method of monitoring arterial oxygen saturation during general anaesthesia in this species. Adapting the oxygen saturation algorithm to consider the left shift of the ODC provides a useful tool for monitoring oxygen status, especially as pulse oximetry is insufficiently accurate. Experimental determination of the complete Hill curve is required to further validate and optimize the algorithm for use in the white rhinoceros. The method will facilitate the accurate interpretation of oxygen saturation calculated by blood gas analysis in white rhinoceros. © 2016 Association of Veterinary Anaesthetists and the American

  2. Semen collection in rhinoceroses (Rhinoceros unicornis, Diceros bicornis, Ceratotherium simum) by electroejaculation with a uniquely designed probe.

    Science.gov (United States)

    Roth, Terri L; Stoops, Monica A; Atkinson, Mark W; Blumer, Evan S; Campbell, Mark K; Cameron, Ken N; Citino, Scott B; Maas, Adolf K

    2005-12-01

    Electroejaculation in rhinoceroses has historically yielded inconsistent results, with the collection of high-quality, sperm-rich samples rare. The goal of this study was to develop a reliable method of electroejaculation in the rhinoceros by designing a rectal probe that appropriately fits the anatomy of this taxon and refining the procedure. A curved probe handle ending in an oblate, ellipsoid head was built using readily available supplies. A combination of rectal massage, penile massage, and electrical stimulation with a specially designed probe was employed in attempts to collect semen on 14 occasions from greater one-horned rhinoceroses (Rhinoceros unicornis; n = 4), black rhinoceroses (Diceros bicornis; n = 2) and a southern white rhinoceros (Ceratotherium simum; n = 1). During 13 of the 14 attempts, ejaculates were collected in multiple fractions. All but one of the ejaculates contained spermatozoa, and seven ejaculates contained good-quality fractions of semen (-60% sperm motility; > or =20 x 106 spermatozoa/ml) suitable for sperm banking and assisted reproduction procedures. Mean (+/-SEM) values for volume, pH, osmolality, and total sperm number for ejaculates containing good-quality fractions (98.2 +/-21.8 ml, 8.5+/-0.1, 290.4+/-6.7 mOsm, and 37.1+/-12.0 x 10(9), respectively) did not differ (P > 0.05) from those containing only poor-quality samples. Urine and/or erythrocyte contamination was not uncommon in fractions of both ejaculate types. Males producing good-quality samples ranged in age from 7 to 34 yr. None of the samples contained > or =75% morphologically normal spermatozoa. Electroejaculation with a uniquely designed probe consistently produced ejaculates in the rhinoceros. However, the production of high-quality samples continued to be challenging, occurring in only 50% of collection attempts. Regardless, the technology has progressed to a stage at which good-quality semen samples can be produced for sperm banking and assisted reproduction

  3. Japanese Interest in “Hotaru” (Fireflies and “Kabuto-Mushi” (Japanese Rhinoceros Beetles Corresponds with Seasonality in Visible Abundance

    Directory of Open Access Journals (Sweden)

    Kenta Takada

    2012-04-01

    Full Text Available Seasonal changes in the popularity of fireflies [usually Genji-fireflies (Luciola cruciata Motschulsky in Japan] and Japanese rhinoceros beetles [Allomyrina dichotoma (Linne] were investigated to examine whether contemporary Japanese are interested in visible emergence of these insects as seasonal events. The popularity of fireflies and Japanese rhinoceros beetles was assessed by the Google search volume of their Japanese names, “Hotaru” and “Kabuto-mushi” in Japanese Katakana script using Google Trends. The search volume index for fireflies and Japanese rhinoceros beetles was distributed across seasons with a clear peak in only particular times of each year from 2004 to 2011. In addition, the seasonal peak of popularity for fireflies occurred at the beginning of June, whereas that for Japanese rhinoceros beetles occurred from the middle of July to the beginning of August. Thus seasonal peak of each species coincided with the peak period of the emergence of each adult stage. These findings indicated that the Japanese are interested in these insects primarily during the time when the two species are most visibly abundant. Although untested, this could suggest that fireflies and Japanese rhinoceros beetles are perceived by the general public as indicators or symbols of summer in Japan.

  4. A Multiplex PCR Assay for Differentiating Coconut Rhinoceros Beetle (Coleoptera: Scarabaeidae) From Oriental Flower Beetle (Coleoptera: Scarabaeidae) in Early Life Stages and Excrement.

    Science.gov (United States)

    Watanabe, S; Melzer, M J

    2017-04-01

    The coconut rhinoceros beetle, Oryctes rhinoceros (L.), is a major pest of coconut and other palm trees. An incipient coconut rhinoceros beetle population was recently discovered on the island of Oahu, Hawaii and is currently the target of a large, mutiagency eradication program. Confounding this program is the widespread presence of another scarab beetle on Oahu, the oriental flower beetle, Protaetia orientalis (Gory and Percheron 1833). Eggs, early life stages, and fecal excrement of coconut rhinoceros beetle and oriental flower beetle are morphologically indistinguishable, thereby creating uncertainty when such specimens are discovered in the field. Here, we report the development of a multiplex PCR assay targeting cytochrome oxidase I of coconut rhinoceros beetle and oriental flower beetle that can rapidly detect and distinguish between these insects. This assay also features an internal positive control to ensure DNA of sufficient quantity and quality is used in the assay, increasing its reliability and reducing the chances of false negative results. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. A comparison of woody browse selection by hand-raised, boma-adapted and wild black rhinoceros, Diceros bicornis, L. in Matusadona National Park, Zimbabwe

    Directory of Open Access Journals (Sweden)

    G. Matipano

    2003-12-01

    Full Text Available Differences in woody browse selection between hand-raised (and subsequently released, boma-adapted and wild black rhinoceros, Diceros bicornis, L. were studied in Matusadona National Park between December 1999 and July 2000. Boma-adapted rhinoceros were animals that were subjected to hand-raising and were kept in bomas (enclosures over night. The feeding behaviour was different between the three rhinoceros groups. All rhinoceros groups utilised and selected for a few browse species in common, at different preference levels in the same habitat types according to season. Wild rhinoceroses browsed most in Colophospermum-Terminalia-Combretum woodland in the wet season and in thicket in the dry season. Hand-raised rhinoceroses browsed most in Colophospermum-Terminalia-Combretum woodland and boma-adated rhinos in thickets in both the wet and the early dry seasons. Hand-raised and bomaadapted rhinos changed their habitats less for browse selection than wild rhinos. This can be ascribed to a relative restriction of home range in the hand-raised group and a herding effect for the boma-adapted animals. These situations might have accounted for differences in seasonal browse selection by the rhinoceros groups.

  6. The impact of the Krakatoa eruption in 1883 on the population of Rhinoceros sondaicus in Ujung Kulon, with details of rhino observations from 1857 to 1949

    Directory of Open Access Journals (Sweden)

    N.J.van. Strien

    2010-01-01

    Full Text Available A recent suggestion that the entire population of the Javan Rhinoceros in Ujung Kulon National Park was annihilated by the effects of the eruption of the Krakatoa in 1883 is investigated. Based on a review of contemporary reports, it is shown that people survived the waves and remained settled in one village until 1906 when it was evacuated during a plague of tigers. The first report of a rhinoceros in the peninsula of Ujung Kulon dates from 1857 and the animals were occasionally reported from the area afterwards. There is no indication from the available estimates and sightings that rhinos were exterminated in the area. Ujung Kulon has been a protected area since 1921. Rhino numbers ranged upwards to about 40 or 50 for most of the period until 1949. The population of Rhinoceros sondaicus is not a new founder population established after the eruption of the Krakatoa in 1883.

  7. Rectal prolapse associated with a healed pelvic fracture in a pregnant free-ranging African black rhinoceros (Diceros bicornis. Part 2 : surgery and necropsy : case report

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    A. Olivier

    2001-07-01

    Full Text Available The oedematous and traumatised protruding section of the rectal tissue of an adult free-ranging female African black rhinoceros (Diceros bicornis was surgically amputated. Immediately before completion of surgery, the rhinoceros died of anaesthetic-related cardiac arrest. At necropsy a deformed pelvis and sacrum associated with a healed fracture of the left ileal wing were noted. New bone formation in and around the left ventral sacral foramina may have resulted in neuropathy of particularly the 3rd and 4th left ventral sacral nerves, which (in the horse supply the majority of the nerve fibres innervating the caudal rectum and anus. The cause of the injury is not known, although back injuries, presumably sustained during mating by bulls, have been recorded in white rhinoceros. An encounter with elephants could also have been responsible for the injury in this case.

  8. Feasibility Study of NMR Based Serum Metabolomic Profiling to Animal Health Monitoring: A Case Study on Iron Storage Disease in Captive Sumatran Rhinoceros (Dicerorhinus sumatrensis.

    Directory of Open Access Journals (Sweden)

    Miki Watanabe

    Full Text Available A variety of wildlife species maintained in captivity are susceptible to iron storage disease (ISD, or hemochromatosis, a disease resulting from the deposition of excess iron into insoluble iron clusters in soft tissue. Sumatran rhinoceros (Dicerorhinus sumatrensis is one of the rhinoceros species that has evolutionarily adapted to a low-iron diet and is susceptible to iron overload. Hemosiderosis is reported at necropsy in many African black and Sumatran rhinoceroses but only a small number of animals reportedly die from hemochromatosis. The underlying cause and reasons for differences in susceptibility to hemochromatosis within the taxon remains unclear. Although serum ferritin concentrations have been useful in monitoring the progression of ISD in many species, there is some question regarding their value in diagnosing hemochromatosis in the Sumatran rhino. To investigate the metabolic changes during the development of hemochromatosis and possibly increase our understanding of its progression and individual susceptibility differences, the serum metabolome from a Sumatran rhinoceros was investigated by nuclear magnetic resonance (NMR-based metabolomics. The study involved samples from female rhinoceros at the Cincinnati Zoo (n = 3, including two animals that died from liver failure caused by ISD, and the Sungai Dusun Rhinoceros Conservation Centre in Peninsular Malaysia (n = 4. Principal component analysis was performed to visually and statistically compare the metabolic profiles of the healthy animals. The results indicated that significant differences were present between the animals at the zoo and the animals in the conservation center. A comparison of the 43 serum metabolomes of three zoo rhinoceros showed two distinct groupings, healthy (n = 30 and unhealthy (n = 13. A total of eighteen altered metabolites were identified in healthy versus unhealthy samples. Results strongly suggest that NMR-based metabolomics is a valuable tool for

  9. Feasibility Study of NMR Based Serum Metabolomic Profiling to Animal Health Monitoring: A Case Study on Iron Storage Disease in Captive Sumatran Rhinoceros (Dicerorhinus sumatrensis)

    Science.gov (United States)

    Watanabe, Miki; Roth, Terri L.; Bauer, Stuart J.; Lane, Adam; Romick-Rosendale, Lindsey E.

    2016-01-01

    A variety of wildlife species maintained in captivity are susceptible to iron storage disease (ISD), or hemochromatosis, a disease resulting from the deposition of excess iron into insoluble iron clusters in soft tissue. Sumatran rhinoceros (Dicerorhinus sumatrensis) is one of the rhinoceros species that has evolutionarily adapted to a low-iron diet and is susceptible to iron overload. Hemosiderosis is reported at necropsy in many African black and Sumatran rhinoceroses but only a small number of animals reportedly die from hemochromatosis. The underlying cause and reasons for differences in susceptibility to hemochromatosis within the taxon remains unclear. Although serum ferritin concentrations have been useful in monitoring the progression of ISD in many species, there is some question regarding their value in diagnosing hemochromatosis in the Sumatran rhino. To investigate the metabolic changes during the development of hemochromatosis and possibly increase our understanding of its progression and individual susceptibility differences, the serum metabolome from a Sumatran rhinoceros was investigated by nuclear magnetic resonance (NMR)-based metabolomics. The study involved samples from female rhinoceros at the Cincinnati Zoo (n = 3), including two animals that died from liver failure caused by ISD, and the Sungai Dusun Rhinoceros Conservation Centre in Peninsular Malaysia (n = 4). Principal component analysis was performed to visually and statistically compare the metabolic profiles of the healthy animals. The results indicated that significant differences were present between the animals at the zoo and the animals in the conservation center. A comparison of the 43 serum metabolomes of three zoo rhinoceros showed two distinct groupings, healthy (n = 30) and unhealthy (n = 13). A total of eighteen altered metabolites were identified in healthy versus unhealthy samples. Results strongly suggest that NMR-based metabolomics is a valuable tool for animal health

  10. Amylase production potentials of bacterial isolates obtained from the gut of Oryctes rhinoceros larvae

    Science.gov (United States)

    Aryati, P. C.; Pangastuti, A.; Sari, S. L. A.

    2017-04-01

    Amylase is one of the main enzymes used in industry, such as food, detergent, textile, and pharmaceutical industry. Amylase can be produced by plants, animals, and microorganisms. However, bacterial and fungal amylases have dominated application in industries. This research was aimed to determine amylolytic activity of bacteria isolated from the gut of Oryctes rhinoceros larvae. Based on clear zone formation, 9 from 11 isolates showed amylolytic activity. Isolates with the widest clear zone, i.e Bacillus subtilis GOR1, Bacillus cereus GOR3, and Bacillus pumilus GOR2, were screened for amylolytic activity based on reduction sugar production. The result showed that Bacillus subtilis GOR1 was the most potential as amylase producer, showed by the widest clear zone 5.224 cm2 and highest reduction sugar production 0.0235 mg/ml. Highest amylase specific activity (0.1447 U/mg protein) was obtained at 60°C and pH 7. Amylase activity was stable for 3 hours at 60°C with residual activity respectively was 59.7%.

  11. Two new entodiniomorphid Triplumaria ciliates from the intestine of the wild African white rhinoceros.

    Science.gov (United States)

    Ito, Akira; Van Hoven, Wouter; Miyazaki, Yutaka; Imai, Soichi

    2008-05-01

    Two new species of Triplumaria in the order Entodiniomorphida, T. alluvia n. sp. and T. grypoclunis n. sp., are described from the large intestine of the wild African white rhinoceros. T. alluvia has three bud-shaped caudalia, one broad skeletal plate with a wavy left dorsal edge, and an axe-shaped tail flap. T. grypoclunis has three short arched caudalia, two broad skeletal plates, and a pointed and ventrally curved tail flap. These two new species have a C-shaped adoral polybrachykinety, a slender perivestibular polybrachykinety, and paralabial kineties in their retractable adoral ciliary zone. In T. alluvia, the perivestibular polybrachykinety is joined to both ends of the adoral polybrachykinety and paralabial kineties along the ventral side of the adoral polybrachykinety, showing the same arrangement as in Cycloposthium species. In T. grypoclunis, the perivestibular polybrachykinety is joined only to the right end of the adoral polybrachykinety and paralabial kineties along the left ventral side of the adoral polybrachykinety, showing an arrangement analogous to the Tripalmaria species.

  12. Landscape preference of the white rhinoceros in the central and northern Kruger National Park

    Directory of Open Access Journals (Sweden)

    D.J. Pienaar

    1993-09-01

    Full Text Available The long-term landscape preferences of the white rhinoceros for 32 different landscapes in the central and northern Kruger National Park are investigated. A preference index and a chi-square test are used to ascertain if white rhinoceroses prefer or avoid a particular landscape as habitat. Landscapes 13 (Karoo Sediment Plains with Acacia welwitschii Tree Savanna and 11 (Slightly Undulating Granitoid Plains with Colophospermum mopane Bush Savanna, are the most preferred landscapes. Landscapes 23 (Basaltic Plains with Colophospermum mopane Shrub Savanna, 25 (Moderately Undulating Gabbroic Plains with Colophospermum mopane Shrub Savanna, 26 (Irregular Calsitic Plains with Colophospermum mopane Shrub Savanna, 28 (Alluvial Plains with Acacia albida Tree Savanna, 32 (Recent Sandy Plains with Baphia massaiensis Bush Savanna and 33 (Slightly Undulating Andesitic Plains with Comhretum collinum Shrub Savanna appear to be avoided. Characterestics of the preferred landscapes are: moderate to dense grass cover with good quality grasses; open to moderate low-shrub (<2 m stratum; a moderate tree stratum; an undulating topography with uplands, bottomlands and watercourses; sandy soils with few stones and rocks on the soil surface; permanent water sources.

  13. Fecal corticosterone concentrations and reproductive success in captive female southern white rhinoceros.

    Science.gov (United States)

    Metrione, Lara C; Harder, John D

    2011-05-01

    Prolonged or frequent secretion of adrenal glucocorticoids in response to aversive stimuli can negatively impact reproduction. Because female southern white rhinoceros (Ceratotherium simum simum) reproduce poorly in captivity, we compared fecal corticosterone metabolite concentrations among parous, nulliparous, and adolescent females and examined social and physical aspects of the captive environment that might be related to differences in corticosterone metabolite concentrations. Aggression, dominance, sexual and play interactions, social group size and composition, enclosure size, and other housing characteristics were assessed though behavioral observations and review of historical and institution records. Concentrations of metabolized corticosterone in fecal samples were analyzed by enzyme immunoassay. The proportion of nulliparous females did not differ (p>0.05) between subordinate and dominant animals, and subordinates did not have a higher mean fecal corticosterone concentration than dominants (p>0.05). Of the behaviors examined, only the frequency of sexual play behaviors differed (p0.05) for females housed in most of the environmental conditions assessed. Housing with a female companion known from adolescence, however, tended to be associated (p=0.06) with a lower mean corticosterone concentration than that when housing with a female companion introduced during adulthood or no female companion. Wild-caught females had a higher (p0.05) between acyclic and cycling, or nulliparous and parous females. Copyright © 2011 Elsevier Inc. All rights reserved.

  14. The sixth rhino: a taxonomic re-assessment of the critically endangered northern white rhinoceros.

    Science.gov (United States)

    Groves, Colin P; Fernando, Prithiviraj; Robovský, Jan

    2010-04-07

    The two forms of white rhinoceros; northern and southern, have had contrasting conservation histories. The Northern form, once fairly numerous is now critically endangered, while the southern form has recovered from a few individuals to a population of a few thousand. Since their last taxonomic assessment over three decades ago, new material and analytical techniques have become available, necessitating a review of available information and re-assessment of the taxonomy. Dental morphology and cranial anatomy clearly diagnosed the southern and northern forms. The differentiation was well supported by dental metrics, cranial growth and craniometry, and corresponded with differences in post-cranial skeleton, external measurements and external features. No distinctive differences were found in the limited descriptions of their behavior and ecology. Fossil history indicated the antiquity of the genus, dating back at least to early Pliocene and evolution into a number of diagnosable forms. The fossil skulls examined fell outside the two extant forms in the craniometric analysis. Genetic divergence between the two forms was consistent across both nuclear and mitochondrial genomes, and indicated a separation of over a million years. On re-assessing the taxonomy of the two forms we find them to be morphologically and genetically distinct, warranting the recognition of the taxa formerly designated as subspecies; Ceratotherium simum simum the southern form and Ceratotherium simum cottoni the northern form, as two distinct species Ceratotherium simum and Ceratotherium cottoni respectively. The recognition of the northern form as a distinct species has profound implications for its conservation.

  15. The Sixth Rhino: A Taxonomic Re-Assessment of the Critically Endangered Northern White Rhinoceros

    Science.gov (United States)

    Groves, Colin P.; Fernando, Prithiviraj; Robovský, Jan

    2010-01-01

    Background The two forms of white rhinoceros; northern and southern, have had contrasting conservation histories. The Northern form, once fairly numerous is now critically endangered, while the southern form has recovered from a few individuals to a population of a few thousand. Since their last taxonomic assessment over three decades ago, new material and analytical techniques have become available, necessitating a review of available information and re-assessment of the taxonomy. Results Dental morphology and cranial anatomy clearly diagnosed the southern and northern forms. The differentiation was well supported by dental metrics, cranial growth and craniometry, and corresponded with differences in post-cranial skeleton, external measurements and external features. No distinctive differences were found in the limited descriptions of their behavior and ecology. Fossil history indicated the antiquity of the genus, dating back at least to early Pliocene and evolution into a number of diagnosable forms. The fossil skulls examined fell outside the two extant forms in the craniometric analysis. Genetic divergence between the two forms was consistent across both nuclear and mitochondrial genomes, and indicated a separation of over a million years. Conclusions On re-assessing the taxonomy of the two forms we find them to be morphologically and genetically distinct, warranting the recognition of the taxa formerly designated as subspecies; Ceratotherium simum simum the southern form and Ceratotherium simum cottoni the northern form, as two distinct species Ceratotherium simum and Ceratotherium cottoni respectively. The recognition of the northern form as a distinct species has profound implications for its conservation. PMID:20383328

  16. TINGKAT KESESUAIAN SUAKA MARGASATWA CIKEPUH SEBAGAI HABITAT KEDUA BADAK JAWA (Rhinoceros sondaicus Desmarest, 1822

    Directory of Open Access Journals (Sweden)

    Ribai .

    2016-01-01

    Full Text Available Javan rhino (Rhinoceros sondaicus is one of the rarest species in the world so categorized as critically endangered by IUCN. Survival of the rhino in Ujung Kulon National Park is threatened by a variety of factors that could cause these extinct animals, such as: invasion langkap, competition with the bulls, and inbreeding. The strategy should be promoted in maintaining and developing population that is making a second habitat. The purpose of this research is to know the suitability level of Cikepuh Wildlife Reserves (CWR as javan rhino’s second habitat. The method used is the field observations. Results showed that the CWR have high suitability as javan rhino’s second habitat with an area of 6886.4 ha (84.72% CWR. Cikepuh Wildlife Reserves components that have a high potential as second habitat are on aspects altitude, air temperature, humidity, water availability, and soil pH. Strategies that can be done in improving the suitability of the CWR as second habitat includ: create pools ofthe rhino, planting food plants that have a high palatability and reduce human pressure through strict enforcement, public education, standardized regular patrols, rehabilitation and enrichment of degraded area, livestock expenses , and review the MoU regarding the use of the area as a military Cikepuh SM. Keywords: Cikepuh Wildlife Reserve, habitat suitability, javan rhino, second habitat

  17. Middle Pleistocene protein sequences from the rhinoceros genus Stephanorhinus and the phylogeny of extant and extinct Middle/Late Pleistocene Rhinocerotidae.

    Science.gov (United States)

    Welker, Frido; Smith, Geoff M; Hutson, Jarod M; Kindler, Lutz; Garcia-Moreno, Alejandro; Villaluenga, Aritza; Turner, Elaine; Gaudzinski-Windheuser, Sabine

    2017-01-01

    Ancient protein sequences are increasingly used to elucidate the phylogenetic relationships between extinct and extant mammalian taxa. Here, we apply these recent developments to Middle Pleistocene bone specimens of the rhinoceros genus Stephanorhinus . No biomolecular sequence data is currently available for this genus, leaving phylogenetic hypotheses on its evolutionary relationships to extant and extinct rhinoceroses untested. Furthermore, recent phylogenies based on Rhinocerotidae (partial or complete) mitochondrial DNA sequences differ in the placement of the Sumatran rhinoceros ( Dicerorhinus sumatrensis ). Therefore, studies utilising ancient protein sequences from Middle Pleistocene contexts have the potential to provide further insights into the phylogenetic relationships between extant and extinct species, including Stephanorhinus and Dicerorhinus . ZooMS screening (zooarchaeology by mass spectrometry) was performed on several Late and Middle Pleistocene specimens from the genus Stephanorhinus , subsequently followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to obtain ancient protein sequences from a Middle Pleistocene Stephanorhinus specimen. We performed parallel analysis on a Late Pleistocene woolly rhinoceros specimen and extant species of rhinoceroses, resulting in the availability of protein sequence data for five extant species and two extinct genera. Phylogenetic analysis additionally included all extant Perissodactyla genera ( Equus , Tapirus ), and was conducted using Bayesian (MrBayes) and maximum-likelihood (RAxML) methods. Various ancient proteins were identified in both the Middle and Late Pleistocene rhinoceros samples. Protein degradation and proteome complexity are consistent with an endogenous origin of the identified proteins. Phylogenetic analysis of informative proteins resolved the Perissodactyla phylogeny in agreement with previous studies in regards to the placement of the families Equidae, Tapiridae, and

  18. Middle Pleistocene protein sequences from the rhinoceros genus Stephanorhinus and the phylogeny of extant and extinct Middle/Late Pleistocene Rhinocerotidae

    Directory of Open Access Journals (Sweden)

    Frido Welker

    2017-03-01

    Full Text Available Background Ancient protein sequences are increasingly used to elucidate the phylogenetic relationships between extinct and extant mammalian taxa. Here, we apply these recent developments to Middle Pleistocene bone specimens of the rhinoceros genus Stephanorhinus. No biomolecular sequence data is currently available for this genus, leaving phylogenetic hypotheses on its evolutionary relationships to extant and extinct rhinoceroses untested. Furthermore, recent phylogenies based on Rhinocerotidae (partial or complete mitochondrial DNA sequences differ in the placement of the Sumatran rhinoceros (Dicerorhinus sumatrensis. Therefore, studies utilising ancient protein sequences from Middle Pleistocene contexts have the potential to provide further insights into the phylogenetic relationships between extant and extinct species, including Stephanorhinus and Dicerorhinus. Methods ZooMS screening (zooarchaeology by mass spectrometry was performed on several Late and Middle Pleistocene specimens from the genus Stephanorhinus, subsequently followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS to obtain ancient protein sequences from a Middle Pleistocene Stephanorhinus specimen. We performed parallel analysis on a Late Pleistocene woolly rhinoceros specimen and extant species of rhinoceroses, resulting in the availability of protein sequence data for five extant species and two extinct genera. Phylogenetic analysis additionally included all extant Perissodactyla genera (Equus, Tapirus, and was conducted using Bayesian (MrBayes and maximum-likelihood (RAxML methods. Results Various ancient proteins were identified in both the Middle and Late Pleistocene rhinoceros samples. Protein degradation and proteome complexity are consistent with an endogenous origin of the identified proteins. Phylogenetic analysis of informative proteins resolved the Perissodactyla phylogeny in agreement with previous studies in regards to the placement of the families

  19. Acute hepatic necrosis and death in a subadult southern white rhinoceros (Ceratotherium simum) associated with exposure to sterigmatocystin in forage contaminated with Aspergillus nidulans.

    Science.gov (United States)

    Bryant, B R; Campbell, M; Sangster, C

    2016-11-01

    A young male southern white rhinoceros (Ceratotherium simum), which was resident in a zoo as part of a multi-rhinoceros group, died suddenly. Necropsy and histopathological findings supported a diagnosis of death from acute hepatic necrosis. The microscopic distribution of liver lesions was suggestive of hepatotoxicosis. Further investigation revealed potential exposure to a mycotoxin, sterigmatocystin, present in spoiled lucerne hay contaminated with Aspergillus nidulans. It was concluded that mycotoxicosis was the likely cause of the hepatic necrosis and death in this animal. © 2016 Australian Veterinary Association.

  20. Middle Pleistocene protein sequences from the rhinoceros genus Stephanorhinus and the phylogeny of extant and extinct Middle/Late Pleistocene Rhinocerotidae

    Science.gov (United States)

    Smith, Geoff M.; Hutson, Jarod M.; Kindler, Lutz; Garcia-Moreno, Alejandro; Villaluenga, Aritza; Turner, Elaine

    2017-01-01

    Background Ancient protein sequences are increasingly used to elucidate the phylogenetic relationships between extinct and extant mammalian taxa. Here, we apply these recent developments to Middle Pleistocene bone specimens of the rhinoceros genus Stephanorhinus. No biomolecular sequence data is currently available for this genus, leaving phylogenetic hypotheses on its evolutionary relationships to extant and extinct rhinoceroses untested. Furthermore, recent phylogenies based on Rhinocerotidae (partial or complete) mitochondrial DNA sequences differ in the placement of the Sumatran rhinoceros (Dicerorhinus sumatrensis). Therefore, studies utilising ancient protein sequences from Middle Pleistocene contexts have the potential to provide further insights into the phylogenetic relationships between extant and extinct species, including Stephanorhinus and Dicerorhinus. Methods ZooMS screening (zooarchaeology by mass spectrometry) was performed on several Late and Middle Pleistocene specimens from the genus Stephanorhinus, subsequently followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to obtain ancient protein sequences from a Middle Pleistocene Stephanorhinus specimen. We performed parallel analysis on a Late Pleistocene woolly rhinoceros specimen and extant species of rhinoceroses, resulting in the availability of protein sequence data for five extant species and two extinct genera. Phylogenetic analysis additionally included all extant Perissodactyla genera (Equus, Tapirus), and was conducted using Bayesian (MrBayes) and maximum-likelihood (RAxML) methods. Results Various ancient proteins were identified in both the Middle and Late Pleistocene rhinoceros samples. Protein degradation and proteome complexity are consistent with an endogenous origin of the identified proteins. Phylogenetic analysis of informative proteins resolved the Perissodactyla phylogeny in agreement with previous studies in regards to the placement of the families Equidae

  1. A study of the reasons for an increase in poaching of the one-horned Indian rhinoceros in Royal Chitwan National Park, Nepal

    OpenAIRE

    Poudyal, Mahesh

    2005-01-01

    Despite a relatively successful conservation programme for the one-horned rhinoceros in national parks of the Terai region, poaching has been one of the major threats to the survival of the rhinoceros, an endangered species in Nepal. This study uses a model to explain the level of poaching in Royal Chitwan National Park (RCNP) over a 30-year period, based on the factors that are thought to influence poaching in the Terai. The results indicate that anti-poaching units (APUs), in their original...

  2. The relationship between vaginal electrical impedence and hormone profiles during pregnancy and parturition of a white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    Bowers, Susan; Gandy, Scott; Paul, Ken; Woods, Lisa; D'Angelo, Denise; Horton, Carolyn; Tabaka, Chris; Willard, Scott

    2005-09-01

    In this study, an attempt was made to use vaginal electrical impedance to predict calving in a female white rhinoceros (Ceratotherium simum simum) and to determine the relationship between vaginal electrical impedance and hormonal profiles during pregnancy. The principle behind vaginal electrical impedance is that a change in the ionic balance of vaginal and cervical mucus occurs in response to changes in reproductive hormones. Three times weekly vaginal electrical impedance readings and fecal samples were collected from midgestation to calving (a 6-mo period). The extracted fecal samples were analyzed for immunoreactive estrogens, progestagens, and corticoids by RIA. Vaginal electrical impedance readings did not decrease before calving but remained consistent throughout the last 140 days of pregnancy. Fecal progestagens in the white rhinoceros decreased between day 17 and day 1 before calving, whereas estrogens increased between 4 and 2 mo before calving, with an additional increase occurring 1 mo before calving. Fecal corticoids increased 5 mo before calving, slowly declined, and increased again within 3 wk before calving. A decline in vaginal electrical impedance was noted 168 days before calving and remained at low levels for 4 wk. At the time of this decrease, the female became aggressive toward the male and began lactating. Fecal progestagens and estrogens did not change during this time; however, fecal corticoids increased as vaginal electrical impedance readings returned to normal along with her behavior and cessation of lactation. In summary, the use of vaginal electrical impedance could not predict parturition in the white rhinoceros. However, an anomaly occurred during pregnancy that was supported by vaginal electrical impedance readings, a change in female behavior, premature lactation, and a subsequent increase in fecal corticoids. The etiology of this physiological anomaly is unknown, yet it did not compromise pregnancy.

  3. Towards establishing a rhinoceros-specific interferon-gamma (IFN-γ) assay for diagnosis of tuberculosis.

    Science.gov (United States)

    Morar, D; Schreuder, J; Mény, M; van Kooten, P J S; Tijhaar, E; Michel, A L; Rutten, V P M G

    2013-11-01

    Mycobacterium bovis is the causal agent of bovine tuberculosis (BTB), with a diverse host range, extending from livestock to domestic and captive wild animals as well as free-ranging wildlife species. In South Africa, BTB is endemic in the Kruger National Park (KNP) and the Hluluwe iMfolozi National Park (HiP), where the high prevalence of M. bovis infections in buffalo herds has led to infection of a number of wildlife species. This has raised concerns about the spillover into the rhinoceros population, a species known to be susceptible to both M. bovis and Mycobacterium tuberculosis, jeopardizing breeding and relocation projects that serve to conserve and protect this species. In view of the advantages of the interferon-gamma (IFN-γ) assay in the diagnosis of BTB in a variety of species worldwide, such an assay has been developed for rhinoceroses by Morar and co-workers in 2007. In this study, this assay was optimized using recombinant eukaryotic rhinoceros IFN-γ and the lower detection limit was calculated to be 0.5 ng/ml. Subsequently, assessing the detection of native rhinoceros IFN-γ protein in whole-blood samples revealed stimulation with each of the mitogens: pokeweed (PWM), phytohaemagglutinin (PHA) & phorbol 12-myristate 13-acetate and calcium ionophore (PMA/CaI), though most prominently with the latter two. In addition, samples collected from 52 clinically healthy rhinoceroses, of presumed negative BTB status, from two different areas in South Africa were used to determine the cut-off value for a negative test result. This was calculated to be 0.10 (OD490 nm ) and as determined in this study is a preliminary recommendation based on IFN-γ responses observed in samples from BTB-free rhinoceroses only. © 2013 Blackwell Verlag GmbH.

  4. Vaccine-induced protection against anthrax in cheetah (Acinonyx jubatus) and black rhinoceros (Diceros bicornis).

    Science.gov (United States)

    Turnbull, P C B; Tindall, B W; Coetzee, J D; Conradie, C M; Bull, R L; Lindeque, P M; Huebschle, O J B

    2004-09-03

    Institution of a policy of vaccination in endangered species with a vaccine not previously administered to it cannot be undertaken lightly. This applies even more in the case of cheetah (Acinonyx jubatus) with their unusually monomorphic gene pool and the potential restrictions this places on their immune responses. However, the recently observed mortalities from anthrax in these animals in the Etosha National Park, Namibia, made it imperative to evaluate vaccination. Black rhinoceros (Diceros bicornis), another endangered species in the park, have been vaccinated for over three decades but the effectiveness of this has never been evaluated. Passive protection tests in A/J mice using sera from 12 cheetahs together with enzyme immunoassay indicated that cheetah are able to mount seemingly normal primary and secondary humoral immune responses to the Sterne 34F2 live spore livestock vaccine. Overall protection rates in mice injected with the sera rose and fell in concert with rises and declines in antibody titres, although fine analysis showed that the correlation between titre and protection was complex. Once a high level of protection (96% of mice 1 month after a second booster in the cheetahs) had been achieved, the duration of substantial protection appeared good (60% of the mice 5 months after the second booster). Protection conferred on mice by sera from three of four vaccinated rhino was almost complete, but, obscurely, none of the mice receiving serum from the fourth rhino were protected. Sera from three park lions with naturally acquired high antibody titres, included as controls, also conferred high levels of protection. For the purposes of wildlife management, the conclusions were that vaccination of cheetah with the standard animal anthrax vaccine causes no observable ill effect in the animals and does appear to confer protective immunity. At least one well-separated booster does appear to be desirable. Vaccination of rhino also appears to be justified

  5. Contact calls of the northern and southern white rhinoceros allow for individual and species identification.

    Science.gov (United States)

    Cinková, Ivana; Policht, Richard

    2014-01-01

    Inter-individual relationships particularly in socially living mammals often require a well-developed communication system. Vocal and olfactory signals are the most important for the communication of rhinos, however, their vocal communication has been investigated to a very limited extent so far. White rhinos have the most developed social system out of all the rhinoceros species and vocal signals might therefore play an important role in their social interactions. We recorded repetitive contact pant calls from six captive northern white rhinos (Ceratotherium cottoni) and 14 captive and free-ranging southern white rhinos (Ceratotherium simum) and examined if they transmit information about individual identity, species, social context and age class. Discriminant analyses revealed that a high percentage of the pant calls of both species could be classified to a correct individual. We calculated signature information capacity of pant calls recorded from adult animals in isolation at 3.19 bits for the northern white rhinos and at 3.15 bits for the southern white rhinos, which can potentially allow for a vocal discrimination of nine individuals of both species. We found that pant calls varied by species. Northern white rhinos had longer calls and also differed from the southern white rhinos in several frequency parameters of their calls. We also analysed the pant calls of southern white rhinos for the differences between the age classes and between social contexts in which they were recorded. Our results show that pant calls carry information about individual, species, age class and context. The ability to recognize this information would allow rhinos, in addition to olfactory cues, to communicate with highly increased accuracy. A better understanding of communication of white rhinos has potential practical use in their management and conservation particularly because of the low breeding success of white rhinos in captivity.

  6. Estrogenicity of captive southern white rhinoceros diets and their association with fertility.

    Science.gov (United States)

    Tubbs, Christopher W; Moley, Laura A; Ivy, Jamie A; Metrione, Lara C; LaClaire, Sydney; Felton, Rachel G; Durrant, Barbara S; Milnes, Matthew R

    2016-11-01

    The captive southern white rhinoceros (SWR) population is not currently self-sustaining, primarily due to poor or absent reproduction of captive-born (F 1+ ) females. In this study, we investigate the role of dietary phytoestrogens in this reproductive phenomenon by characterizing activation of SWR estrogen receptors (ESRs) 1 and 2 by diet items from nine North American institutions and comparing female SWR fertility to total diet estrogenicity. Of the diet items tested, alfalfa hay and soy and alfalfa-based commercial pellets were found to be the most potent activators of SWR ESRs. In contrast, most grass hays tested were not estrogenic. The estrogenicity of total diets varied across the institutions surveyed and the degree of diet estrogenicity was positively associated with the percentage of the total diet comprised by pellets. Comparisons of fertility records of the institutions surveyed showed no significant relationship between diet estrogenicity and fertility for female SWR conceived or born in the wild (F 0 ). However, for F 1+ females, there was a significant negative relationship between institutional diet estrogenicity and fertility. Taken together, these data suggest that developmental exposure to phytoestrogens may be the cause of poor fertility in captive-born female SWR. Whether the low fertility of the current population of captive-born female SWR is permanent or can be reversed by removing phytoestrogens from the diet remains unclear. However, our findings suggest that in order for the SWR population to become self-sustaining, the development and feeding of low phytoestrogen diets should be strongly considered. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. The sixth rhino: a taxonomic re-assessment of the critically endangered northern white rhinoceros.

    Directory of Open Access Journals (Sweden)

    Colin P Groves

    Full Text Available BACKGROUND: The two forms of white rhinoceros; northern and southern, have had contrasting conservation histories. The Northern form, once fairly numerous is now critically endangered, while the southern form has recovered from a few individuals to a population of a few thousand. Since their last taxonomic assessment over three decades ago, new material and analytical techniques have become available, necessitating a review of available information and re-assessment of the taxonomy. RESULTS: Dental morphology and cranial anatomy clearly diagnosed the southern and northern forms. The differentiation was well supported by dental metrics, cranial growth and craniometry, and corresponded with differences in post-cranial skeleton, external measurements and external features. No distinctive differences were found in the limited descriptions of their behavior and ecology. Fossil history indicated the antiquity of the genus, dating back at least to early Pliocene and evolution into a number of diagnosable forms. The fossil skulls examined fell outside the two extant forms in the craniometric analysis. Genetic divergence between the two forms was consistent across both nuclear and mitochondrial genomes, and indicated a separation of over a million years. CONCLUSIONS: On re-assessing the taxonomy of the two forms we find them to be morphologically and genetically distinct, warranting the recognition of the taxa formerly designated as subspecies; Ceratotherium simum simum the southern form and Ceratotherium simum cottoni the northern form, as two distinct species Ceratotherium simum and Ceratotherium cottoni respectively. The recognition of the northern form as a distinct species has profound implications for its conservation.

  8. Contact calls of the northern and southern white rhinoceros allow for individual and species identification.

    Directory of Open Access Journals (Sweden)

    Ivana Cinková

    Full Text Available Inter-individual relationships particularly in socially living mammals often require a well-developed communication system. Vocal and olfactory signals are the most important for the communication of rhinos, however, their vocal communication has been investigated to a very limited extent so far. White rhinos have the most developed social system out of all the rhinoceros species and vocal signals might therefore play an important role in their social interactions. We recorded repetitive contact pant calls from six captive northern white rhinos (Ceratotherium cottoni and 14 captive and free-ranging southern white rhinos (Ceratotherium simum and examined if they transmit information about individual identity, species, social context and age class. Discriminant analyses revealed that a high percentage of the pant calls of both species could be classified to a correct individual. We calculated signature information capacity of pant calls recorded from adult animals in isolation at 3.19 bits for the northern white rhinos and at 3.15 bits for the southern white rhinos, which can potentially allow for a vocal discrimination of nine individuals of both species. We found that pant calls varied by species. Northern white rhinos had longer calls and also differed from the southern white rhinos in several frequency parameters of their calls. We also analysed the pant calls of southern white rhinos for the differences between the age classes and between social contexts in which they were recorded. Our results show that pant calls carry information about individual, species, age class and context. The ability to recognize this information would allow rhinos, in addition to olfactory cues, to communicate with highly increased accuracy. A better understanding of communication of white rhinos has potential practical use in their management and conservation particularly because of the low breeding success of white rhinos in captivity.

  9. Butorphanol with oxygen insufflation improves cardiorespiratory function in field-immobilised white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Haw, Anna; Hofmeyr, Markus; Fuller, Andrea; Buss, Peter; Miller, Michele; Fleming, Gregory; Meyer, Leith

    2015-08-12

    Opioid-induced immobilisation results in severe respiratory compromise in the white rhinoceros (Ceratotherium simum). The effectiveness of oxygen insufflation combined with butorphanol in alleviating respiratory depression in free-ranging chemically immobilised white rhinoceroses was investigated. In this prospective intervention study 14 free-ranging white rhinoceroses were immobilised with a combination of etorphine, azaperone and hyaluronidase. Six minutes (min) after the animals became recumbent, intravenous butorphanol was administered and oxygen insufflation was initiated. Previous boma trial results were used for comparison, using repeated measures two-way analysis of variance. The initial immobilisation-induced hypoxaemia in free-ranging rhinoceroses (arterial partial pressure of oxygen [PaO2] 35.4 mmHg ± 6.6 mmHg) was similar to that observed in boma-confined rhinoceroses (PaO2 31 mmHg ± 6 mmHg, n = 8). Although the initial hypercapnia (PaCO2 63.0 mmHg ± 7.5 mmHg) was not as severe as that in animals in the boma trial (79 mmHg ± 7 mmHg), the field-immobilised rhinoceroses were more acidaemic (pH 7.10 ± 0.14) at the beginning of the immobilisation compared with boma-immobilised rhinoceroses (pH 7.28 ± 0.04). Compared with pre-intervention values, butorphanol with oxygen insufflation improved the PaO2 (81.2 mmHg ± 23.7 mmHg, p white rhinoceroses.

  10. Javan Rhino (Rhinoceros sondaicus Desmarest 1822 Utilization Distribution and Habitat Selection in Ujung Kulon National Park

    Directory of Open Access Journals (Sweden)

    Yanto Santosa

    2013-04-01

    Full Text Available Javan rhino (Rhinoceros sondaicus Desmarest 1822 is one of world's rarest endangered species (IUCN 2008. Currently,  their distribution  is  limited  to Ujung Kulon National Park  (UKNP of  Indonesia. Natural disasters, disease, poaching, encroachment, and competition are threats  facing Javan rhino's population, hence creating pockets of habitats outside  the UKNP was necessary and  should be based on  scientific  studies of  javan  rhino utilization distribution and habitat selection in UKNP as the natural habitat. The objectives of the study were to determine  the utilization distribution of Javan rhino and  its habitat characteristics and determine  javan rhino habitat selection within UKNP.  The research  indicated  that Javan rhinos highest use of areas comprised of swampy shrub (w = 2.197 and shrub (w = 2.012, relatively open habitat (w = 4.233, flat area (w = 1.313 and slightly sloping areas (w = 1.007, lowland (w = 1.346, close to river (w = 1.087, close to road network (w = 1.395, close to coasts/beaches (w = 1.862, close  to wallowing areas (w = 3.709, and close  to rumpang (w = 3.783.  The results suggested  that  javan  rhino  showed  selection and preferences  for  its habitat.Keywords:  javan  rhino, utilization distribution, habitat  selection, preferences

  11. Standardising Home Range Studies for Improved Management of the Critically Endangered Black Rhinoceros.

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    Roan D Plotz

    Full Text Available Comparisons of recent estimations of home range sizes for the critically endangered black rhinoceros in Hluhluwe-iMfolozi Park (HiP, South Africa, with historical estimates led reports of a substantial (54% increase, attributed to over-stocking and habitat deterioration that has far-reaching implications for rhino conservation. Other reports, however, suggest the increase is more likely an artefact caused by applying various home range estimators to non-standardised datasets. We collected 1939 locations of 25 black rhino over six years (2004-2009 to estimate annual home ranges and evaluate the hypothesis that they have increased in size. A minimum of 30 and 25 locations were required for accurate 95% MCP estimation of home range of adult rhinos, during the dry and wet seasons respectively. Forty and 55 locations were required for adult female and male annual MCP home ranges, respectively, and 30 locations were necessary for estimating 90% bivariate kernel home ranges accurately. Average annual 95% bivariate kernel home ranges were 20.4 ± 1.2 km(2, 53 ± 1.9% larger than 95% MCP ranges (9.8 km(2 ± 0.9. When home range techniques used during the late-1960s in HiP were applied to our dataset, estimates were similar, indicating that ranges have not changed substantially in 50 years. Inaccurate, non-standardised, home range estimates and their comparison have the potential to mislead black rhino population management. We recommend that more care be taken to collect adequate numbers of rhino locations within standardized time periods (i.e., season or year and that the comparison of home ranges estimated using dissimilar procedures be avoided. Home range studies of black rhino have been data deficient and procedurally inconsistent. Standardisation of methods is required.

  12. The genome of Oryctes rhinoceros nudivirus provides novel insight into the evolution of nuclear arthropod-specific large circular double-tranded DNA viruses

    NARCIS (Netherlands)

    Wang, Yongjie; Bininda-Emonds, O.R.P.; Oers, van M.M.; Vlak, J.M.; Jehle, J.A.

    2011-01-01

    The Oryctes rhinoceros nudivirus (OrNV) is a dsDNA virus with enveloped, rod-shaped virions. Its genome is 127,615 bp in size and contains 139 predicted protein-coding open reading frames (ORFs). In-depth genome sequence comparisons revealed a varying number of shared gene homologues, not only with

  13. Middle Pleistocene protein sequences from the rhinoceros genus Stephanorhinus and the phylogeny of extant and extinct Middle/Late Pleistocene Rhinocerotidae

    OpenAIRE

    Frido Welker; Geoff M. Smith; Jarod M. Hutson; Lutz Kindler; Alejandro Garcia-Moreno; Aritza Villaluenga; Elaine Turner; Sabine Gaudzinski-Windheuser

    2017-01-01

    Background Ancient protein sequences are increasingly used to elucidate the phylogenetic relationships between extinct and extant mammalian taxa. Here, we apply these recent developments to Middle Pleistocene bone specimens of the rhinoceros genus Stephanorhinus. No biomolecular sequence data is currently available for this genus, leaving phylogenetic hypotheses on its evolutionary relationships to extant and extinct rhinoceroses untested. Furthermore, recent phylogenies based on Rhinocerotid...

  14. Range Analysis and Terrain Preference of Adult Southern White Rhinoceros (Ceratotherium simum) in a South African Private Game Reserve: Insights into Carrying Capacity and Future Management.

    Science.gov (United States)

    Thompson, S; Avent, T; Doughty, L S

    2016-01-01

    The Southern white rhinoceros (Ceratotherium simum) is a threatened species, central to the tourism appeal of private game reserves in South Africa. Privately owned reserves in South Africa tend to be smaller than government run reserves such as Kruger National Park. Because of their relatively small size and the often heterogeneous nature of the landscape private game reserve managers benefit from detailed knowledge of white rhinoceros terrain selection preferences, which can be assessed from their ranging behaviours. We collected adult and sub-adult white rhinoceros distribution data over a 15 month period, calculating individual range size using kernel density estimation analysis within a GIS. From this, terrain selectivity was calculated using 50% and 95% kernels to extract terrain composition values. Jacob's correction of the Ivlev's selectivity index was subsequently applied to the terrain composition of each individual to identify trends in selectivity. Results reveal that adult males hold exclusive territories considerably smaller than those found in previous work conducted in "open" or large reserves. Similarly, results for the size of male versus female territories were also not in keeping with those from previous field studies, with males, rather than females, having the larger territory requirement. Terrain selection for both genders and age classes (adult and sub-adult) showed a strong preference for open grassland and avoidance of hill slope and riparian terrains. This research reveals white rhinoceros terrain selection preferences and how they influence range requirements in small, closed reserves. We conclude that this knowledge will be valuable in future white rhinoceros conservation management in small private game reserves, particularly in decisions surrounding removal of surplus individuals or augmentation of existing populations, calculation of reserve carrying capacity and future private reserve acquisition.

  15. Method comparison and generation of plasma biochemistry RIs for the White rhinoceros on a point-of-care and wet chemistry analyzer.

    Science.gov (United States)

    Hooijberg, Emma H; Steenkamp, Gerhard; Buss, Peter; Goddard, Amelia

    2017-06-01

    Plasma biochemistry analysis may aid the management of White rhinoceros with poaching or other injuries. Analyzer- and species-specific RIs are necessary to evaluate health status and identify abnormalities. The purpose of the study was to perform a comparison study between the IDEXX VetTest and Roche Cobas Integra 400 Plus, and to generate analyzer-specific RIs for White rhinoceros according to published guidelines. Comparison was carried out using White rhinoceros plasma samples from healthy and diseased animals. Reference intervals for albumin, ALT, AST, CK, creatinine, GGT, globulins, glucose, phosphorus, total proteins, and urea were determined from a sample group of chemically immobilized healthy adult White rhinoceros from the Kruger National Park, South Africa. There was a significant analytic bias for all analytes between the 2 analyzers. Results for glucose and total protein were clinically equivalent based on total allowable error limits. Reference intervals were generated from 51 individuals (26 male, 25 female) for all analytes except ALT on the VetTest. The type, direction, and magnitude of bias between the VetTest and Cobas appeared to be species- and analyzer-specific, when compared to other studies. These measures of bias may be used for comparability testing. Reference intervals for total proteins (VetTest 77-108 g/L, Cobas 77-110 g/L) and globulins (VetTest 47-79 g/L, Cobas 51-87 g/L) were high compared to other uneven-toed ungulates. Plasma samples from White rhinoceros can be evaluated on both the VetTest and Cobas. Results from this study will aid conservation efforts directed toward this species. © 2017 American Society for Veterinary Clinical Pathology.

  16. Analytic and quality control validation and assessment of field performance of a point-of-care chemistry analyzer for use in the White rhinoceros.

    Science.gov (United States)

    Hooijberg, Emma H; Steenkamp, Gerhard; du Preez, Jacques P; Goddard, Amelia

    2017-03-01

    A chemistry point-of-care analyzer would be useful for evaluating injured wildlife, particularly White rhinoceros (Ceratotherium simum) that survive poaching attempts. The IDEXX VetTest could be suitable, but species-specific validation, development of a statistical quality control (QC) strategy, and evaluation under field conditions are necessary. The objectives were to (1) validate the VetTest for the White rhinoceros, (2) perform QC validation on the VetTest and generate a statistical QC strategy, and (3) apply this QC strategy to monitor performance under typical field conditions. Differences between White rhinoceros heparin plasma and serum, short-term imprecision, and reportable range using rhinoceros plasma and long-term imprecision using commercial quality control material (QCM) were assessed against prescribed total allowable error (TE a ) for up to 15 analytes. Quality control validation was performed using data from the long-term imprecision study and TE a . A QC strategy using QCM was developed and used to monitor performance under field conditions. Imprecision was acceptable for all analytes except for ALP, ALT, and AST at low activities. The reportable range for AST and LDH differed from the manufacturer's specifications. Eleven analytes were suitable for statistical QC using the 1 3s rule, 3 using the 2 s rule; ALP was not suitable. In the field, observed error was 3.0 for 12 analytes. The VetTest is suitable for use in the White rhinoceros. Statistical QC is possible for most analytes and useful for evaluation of field performance. © 2017 American Society for Veterinary Clinical Pathology.

  17. Range Analysis and Terrain Preference of Adult Southern White Rhinoceros (Ceratotherium simum in a South African Private Game Reserve: Insights into Carrying Capacity and Future Management.

    Directory of Open Access Journals (Sweden)

    S Thompson

    Full Text Available The Southern white rhinoceros (Ceratotherium simum is a threatened species, central to the tourism appeal of private game reserves in South Africa. Privately owned reserves in South Africa tend to be smaller than government run reserves such as Kruger National Park. Because of their relatively small size and the often heterogeneous nature of the landscape private game reserve managers benefit from detailed knowledge of white rhinoceros terrain selection preferences, which can be assessed from their ranging behaviours. We collected adult and sub-adult white rhinoceros distribution data over a 15 month period, calculating individual range size using kernel density estimation analysis within a GIS. From this, terrain selectivity was calculated using 50% and 95% kernels to extract terrain composition values. Jacob's correction of the Ivlev's selectivity index was subsequently applied to the terrain composition of each individual to identify trends in selectivity. Results reveal that adult males hold exclusive territories considerably smaller than those found in previous work conducted in "open" or large reserves. Similarly, results for the size of male versus female territories were also not in keeping with those from previous field studies, with males, rather than females, having the larger territory requirement. Terrain selection for both genders and age classes (adult and sub-adult showed a strong preference for open grassland and avoidance of hill slope and riparian terrains. This research reveals white rhinoceros terrain selection preferences and how they influence range requirements in small, closed reserves. We conclude that this knowledge will be valuable in future white rhinoceros conservation management in small private game reserves, particularly in decisions surrounding removal of surplus individuals or augmentation of existing populations, calculation of reserve carrying capacity and future private reserve acquisition.

  18. Identification of policies for a sustainable legal trade in rhinoceros horn based on population projection and socioeconomic models

    Science.gov (United States)

    Di Minin, Enrico; Laitila, Jussi; Montesino-Pouzols, Federico; Leader-Williams, Nigel; Slotow, Rob; Goodman, Peter S; Conway, Anthony J; Moilanen, Atte

    2015-01-01

    Between 1990 and 2007, 15 southern white (Ceratotherium simum simum) and black (Diceros bicornis) rhinoceroses on average were killed illegally every year in South Africa. Since 2007 illegal killing of southern white rhinoceros for their horn has escalated to >950 individuals/year in 2013. We conducted an ecological–economic analysis to determine whether a legal trade in southern white rhinoceros horn could facilitate rhinoceros protection. Generalized linear models were used to examine the socioeconomic drivers of poaching, based on data collected from 1990 to 2013, and to project the total number of rhinoceroses likely to be illegally killed from 2014 to 2023. Rhinoceros population dynamics were then modeled under 8 different policy scenarios that could be implemented to control poaching. We also estimated the economic costs and benefits of each scenario under enhanced enforcement only and a legal trade in rhinoceros horn and used a decision support framework to rank the scenarios with the objective of maintaining the rhinoceros population above its current size while generating profit for local stakeholders. The southern white rhinoceros population was predicted to go extinct in the wild Costo Económico con Referencia Espacial y de Beneficio a la Biodiversidad y la Efectividad de una Estrategia de Determinación de Costos Resumen Entre 1990 y 2007, en promedio fueron cazados ilegalmente cada año 15 rinocerontes sureños blancos (Ceratotherium simum simum) y negros (Diceros bicornis) en Sudáfrica. Desde 2007 la caza ilegal de rinocerontes sureños blancos por su cuerno ha escalado a más de 950 individuos al año en 2013. Llevamos a cabo un análisis ecológico-económico para determinar si el comercio legal de cuerno de rinoceronte sureño blanco podría facilitar la protección del rinoceronte. Se usaron modelos lineales generalizados para examinar a los conductores socio-económicos de la caza furtiva, con base en datos colectados desde 1990 hasta 2013, y

  19. Butorphanol with oxygen insufflation improves cardiorespiratory function in field-immobilised white rhinoceros (Ceratotherium simum

    Directory of Open Access Journals (Sweden)

    Anna Haw

    2015-08-01

    Full Text Available Opioid-induced immobilisation results in severe respiratory compromise in the white rhinoceros (Ceratotherium simum. The effectiveness of oxygen insufflation combined with butorphanol in alleviating respiratory depression in free-ranging chemically immobilised white rhinoceroses was investigated. In this prospective intervention study 14 freeranging white rhinoceroses were immobilised with a combination of etorphine, azaperone and hyaluronidase. Six minutes (min after the animals became recumbent, intravenous butorphanol was administered and oxygen insufflation was initiated. Previous boma trial results were used for comparison, using repeated measures two-way analysis of variance. The initial immobilisation-induced hypoxaemia in free-ranging rhinoceroses (arterial partial pressure of oxygen [PaO2] 35.4 mmHg ± 6.6 mmHg was similar to that observed in bomaconfined rhinoceroses (PaO2 31 mmHg ± 6 mmHg, n = 8. Although the initial hypercapnia (PaCO2 63.0 mmHg ± 7.5 mmHg was not as severe as that in animals in the boma trial (79 mmHg ± 7 mmHg, the field-immobilised rhinoceroses were more acidaemic (pH 7.10 ± 0.14 at the beginning of the immobilisation compared with boma-immobilised rhinoceroses (pH 7.28 ± 0.04. Compared with pre-intervention values, butorphanol with oxygen insufflation improved the PaO2 (81.2 mmHg ± 23.7 mmHg, p < 0.001, 5 min vs 20 min, arterial partial pressure of carbon dioxide (55.3 mmHg ± 5.2 mmHg, p < 0.01, 5 min vs 20 min, pH (7.17 ± 0.11, p < 0.001, 5 min vs 20 min, heart rate (78 breaths/min ± 20 breaths/min, p < 0.001, 5 min vs 20 min and mean arterial blood pressure (105 mmHg ± 14 mmHg, p < 0.01, 5 min vs 20 min. Oxygen insufflation combined with a single intravenous dose of butorphanol improved oxygenation and reduced hypercapnia and acidaemia in immobilised free-ranging white rhinoceroses.

  20. Feasibility of sex-sorting sperm from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis).

    Science.gov (United States)

    Behr, B; Rath, D; Mueller, P; Hildebrandt, T B; Goeritz, F; Braun, B C; Leahy, T; de Graaf, S P; Maxwell, W M C; Hermes, R

    2009-08-01

    The objective of these studies was to investigate the practicality of flow cytometric sex-sorting for spermatozoa from the white and the black rhinoceros (Ceratotherium simum, Diceros bicornis). In Experiment 1, four semen extenders were tested regarding their suitability for liquid preservation of spermatozoa before sorting. Dilution in MES-HEPES-based semen extender followed by incubation generated best sperm quality parameters (motility, viability, and acrosome integrity). In Experiment 2, the effect of staining method (15 degrees C for 4 to 6h during transport or 37 degrees C for 1 to 1.5h) on sort efficiency and sperm quality was investigated. Staining at 15 degrees C during transport resulted in a higher percentage of sperm samples showing a resolution of X- and Y-chromosome-bearing populations (60%) compared with that for staining at 37 degrees C after transport (33%) and resulted in superior sperm integrity after staining (43.8+/-11.3% vs. 19.6+/-12.1%). Sort rate was 300 to 700 cells/sec and sort purity, determined for one sorted sample, was 94% for X-chromosome-bearing spermatozoa. In Experiment 3, the highly viscous component of rhinoceros seminal plasma, which complicates the process of sperm sorting, was examined by gel electrophoresis and mass spectrometry. Results suggested a 250-kDa glycoprotein (most likely originating from the bulbourethral gland) to be responsible for the characteristic viscosity of ejaculates. In Experiment 4, viscosity of seminal plasma, as measured by electron spin resonance spectroscopy, was significantly decreased after addition of alpha-amylase or collagenase (0.5 and 3IU per 100 microL seminal plasma, respectively) by 28% and 21%, respectively, with no negative effect on sperm characteristics. The results of this study demonstrate for the first time that rhinoceros spermatozoa can be successfully sorted into high-purity X- and Y-chromosome-bearing populations. Furthermore, the successful liquefaction of viscous ejaculates

  1. Measurement and characterization of a soundscape of captive southern white rhinoceros (Ceratotherium simum simum) at a wildlife park conservation center

    Science.gov (United States)

    Wiseman, Susan M.

    Many animals, including the myopic rhinoceros, depend on hearing and smell for navigation and to interpret their environment. For them, the "soundscape" and "scentscape" are equivalent to our landscape. Noise damages humans physiologically, including reproductively, and likely damages other mammals. Rhinos vocalize sonically and infrasonically but audiograms are unavailable. Infrasonic noise tends to be chronic in urban areas, which frequently surround city zoos. Rhinos' biological and social management have been studied but little attention, if any, has been paid to their soundscapes. This project develops a standard by which such soundscapes may be measured, documented, and compared, so that once a wide range of rhino facilities have been similarly investigated, correlations could be sought between their sound metrics and the health and well-being of their animals. The interests of geographers overlap many disciplines, but the questions raised by, and the approaches of geographers frequently differ from those addressed by the original specialists, so a broader understanding of the soundscape and ways to record it may well add value to acoustic studies while simultaneously deepening geographic knowledge. This research asks: How can a soundscape of captive southern white rhinoceros (Ceratotherium simum simum) be comprehensively measured and characterized? What does doing so inform about their environment of captivity? How can this method be employed to understand the contrasts of the soundscapes of captivity and natural habitats? To begin to answer these questions, the following goals are addressed: 1. To develop a series of procedures to comprehensively record, measure, analyze, and characterize a broadband white rhino soundscape; 2. To note their vocalizations, and to roughly estimate the bandwidth used by these particular animals; 3. By demonstrating that techniques and language not normally used in the discipline of Geography could broaden its scope and expand

  2. Seasonal variation of diet and faeces composition of Black Rhinoceros Diceros Bicornis in the Addo Elephant National Park

    Directory of Open Access Journals (Sweden)

    A.J Hall-Martin

    1982-11-01

    Full Text Available The feeding habits of black rhinoceros were studied in the Addo Elephant National Park, Republic of South Africa, using the "feeding track" method and recording bites taken. A total sample of 59 feeding tracks, 5 540 plants and 17 191 bites were recorded from June 1976 to March 1977. The rhino fed largely on woody shrubs but also took forbs, grass and succulent plants. During dry periods the rhino were selecting succulent plants with a high moisture content rather than woody plants. A total of 111 plant species were utilized. During dry months the feeding rate was greater than wet months. Physical analysis of faeces composition confirmed conclusions drawn from observations. Chemical analyses of faeces indicated that mean crude protein values varied with rainfall and herbage quality, ash values were strongly influenced by the intake of plant roots and dust during dry periods, acid detergent fibre was highest during unfavourable periods and low during favourable periods.

  3. Seasonal variation of diet and faeces composition of Black Rhinoceros Diceros Bicornis in the Addo Elephant National Park

    Directory of Open Access Journals (Sweden)

    A.J Hall-Martin

    1982-12-01

    Full Text Available The feeding habits of black rhinoceros were studied in the Addo Elephant National Park, Republic of South Africa, using the "feeding track" method and recording bites taken. A total sample of 59 feeding tracks, 5 540 plants and 17 191 bites were recorded from June 1976 to March 1977. The rhino fed largely on woody shrubs but also took forbs, grass and succulent plants. During dry periods the rhino were selecting succulent plants with a high moisture content rather than woody plants. A total of 111 plant species were utilized. During dry months the feeding rate was greater than wet months. Physical analysis of faeces composition confirmed conclusions drawn from observations. Chemical analyses of faeces indicated that mean crude protein values varied with rainfall and herbage quality, ash values were strongly influenced by the intake of plant roots and dust during dry periods, acid detergent fibre was highest during unfavourable periods and low during favourable periods.

  4. Anaesthetic management of a 10-month-old white rhinoceros (Ceratotherium simum calf for emergency exploratory celiotomy

    Directory of Open Access Journals (Sweden)

    Gareth E. Zeiler

    2012-11-01

    Full Text Available A 10-month-old, 580 kg, hand-reared white rhinoceros (Ceratotherium simum calf was presented for emergency exploratory celiotomy. Anaesthesia was safely induced with three successive intravenous (IV boluses of diazepam (10 mg and ketamine (100 mg until the trachea could be intubated. Anaesthesia was adequately maintained with isoflurane-inoxygen (mean end-tidal isoflurane concentration of 1.1% ± 0.2% on a circle anaesthetic machine with carbon dioxide absorption and an intravenous infusion of ketamine and medetomidine at a mean rate of 0.02 mg/kg/min and 0.02 µg/kg/min, respectively. Mean values recorded during anaesthesia and surgery were heart rate (56.9 ± 11 beats/min, mean arterial blood pressure (6.16 kPa ± 1.75 kPa, end-tidal carbon dioxide concentration (6.23 kPa ± 0.30 kPa. Abdominal gas distension contributed to hypoventilation that resulted in hypercapnoea, confirmed by arterial blood gas analysis (PaCO2 14.69 kPa, which required controlled ventilation for correction. Blood volume was maintained with the intravenous infusion of a balanced electrolyte solution at 10 mL/kg/h and blood pressure supported with a continuous infusion of dobutamine and phenylephrine. Duration of anaesthesia was 3.5 h. It was concluded that anaesthesia was safely induced in a compromised white rhinoceros calf with a combination of diazepam and ketamine. A constant-rate infusion of medetomidine and ketamine allowed for a reduction in the dose of isoflurane required during maintenance of anaesthesia and improved intra-operative blood pressure management.

  5. Anaesthetic management of a 10-month-old white rhinoceros (Ceratotherium simum calf for emergency exploratory celiotomy

    Directory of Open Access Journals (Sweden)

    Gareth E. Zeiler

    2012-04-01

    Full Text Available A 10-month-old, 580 kg, hand-reared white rhinoceros (Ceratotherium simum calf was presented for emergency exploratory celiotomy. Anaesthesia was safely induced with three successive intravenous (IV boluses of diazepam (10 mg and ketamine (100 mg until the trachea could be intubated. Anaesthesia was adequately maintained with isoflurane-inoxygen (mean end-tidal isoflurane concentration of 1.1% ± 0.2% on a circle anaesthetic machine with carbon dioxide absorption and an intravenous infusion of ketamine and medetomidine at a mean rate of 0.02 mg/kg/min and 0.02 µg/kg/min, respectively. Mean values recorded during anaesthesia and surgery were heart rate (56.9 ± 11 beats/min, mean arterial blood pressure (6.16 kPa ± 1.75 kPa, end-tidal carbon dioxide concentration (6.23 kPa ± 0.30 kPa. Abdominal gas distension contributed to hypoventilation that resulted in hypercapnoea, confirmed by arterial blood gas analysis (PaCO2 14.69 kPa, which required controlled ventilation for correction. Blood volume was maintained with the intravenous infusion of a balanced electrolyte solution at 10 mL/kg/h and blood pressure supported with a continuous infusion of dobutamine and phenylephrine. Duration of anaesthesia was 3.5 h. It was concluded that anaesthesia was safely induced in a compromised white rhinoceros calf with a combination of diazepam and ketamine. A constant-rate infusion of medetomidine and ketamine allowed for a reduction in the dose of isoflurane required during maintenance of anaesthesia and improved intra-operative blood pressure management.

  6. Anaesthetic management of a 10-month-old white rhinoceros (Ceratotherium simum) calf for emergency exploratory celiotomy.

    Science.gov (United States)

    Zeiler, Gareth E; Stegmann, George F

    2012-11-29

    A 10-month-old, 580 kg, hand-reared white rhinoceros (Ceratotherium simum) calf was presented for emergency exploratory celiotomy. Anaesthesia was safely induced with three successive intravenous (IV) boluses of diazepam (10 mg) and ketamine (100 mg) until the trachea could be intubated. Anaesthesia was adequately maintained with isoflurane-inoxygen (mean end-tidal isoflurane concentration of 1.1% ± 0.2%) on a circle anaesthetic machine with carbon dioxide absorption and an intravenous infusion of ketamine and medetomidine at a mean rate of 0.02 mg/kg/min and 0.02 µg/kg/min, respectively. Mean values recorded during anaesthesia and surgery were heart rate (56.9 ± 11 beats/min), mean arterial blood pressure (6.16 kPa ± 1.75 kPa), end-tidal carbon dioxide concentration (6.23 kPa ± 0.30 kPa). Abdominal gas distension contributed to hypoventilation that resulted in hypercapnoea, confirmed by arterial blood gas analysis (PaCO2 14.69 kPa), which required controlled ventilation for correction. Blood volume was maintained with the intravenous infusion of a balanced electrolyte solution at 10 mL/kg/h and blood pressure supported with a continuous infusion of dobutamine and phenylephrine. Duration of anaesthesia was 3.5 h. It was concluded that anaesthesia was safely induced in a compromised white rhinoceros calf with a combination of diazepam and ketamine. A constant-rate infusion of medetomidine and ketamine allowed for a reduction in the dose of isoflurane required during maintenance of anaesthesia and improved intra-operative blood pressure management.

  7. CLINICAL MANAGEMENT OF BILATERAL CUTANEOUS SQUAMOUS CELL CARCINOMA OF THE HIND FEET PADS IN A SOUTHERN WHITE RHINOCEROS (CERATOTHERIUM SIMUM SIMUM).

    Science.gov (United States)

    Greunz, Eva Maria; Simon, Marie; Lemberger, Karin; Galateanu, Gabriela; Hermes, Robert; Leclerc, Antoine

    2016-06-01

    The current report describes the temporary regression, due to intensive symptomatic treatment, of ulcerative skin lesions caused by squamous cell carcinoma in a white rhinoceros. A captive, 40-yr-old southern white rhinoceros (Ceratotherium simum simum) developed profound, ulcerative skin lesions on the pads of both hind feet. At the peak of the disease, at least one quarter of the pads was affected. A diagnosis of squamous cell carcinoma was made via biopsy. Treatment included anti-inflammatory drugs, antibiotics, and local care. The lesions regressed on both feet until they seemed clinically healed. It was presumed that long-term, anti-inflammatory treatment and local bandaging had induced the temporary regression of the lesions. Two years later, however, a small ulcerative lesion reappeared on one pad and post mortem examination confirmed that the carcinoma was also histologically present in the clinically intact tissue. No metastasis was found and computed tomography showed normal digital bones.

  8. Abundance of food plant species and food habits of Rhinoceros unicorns Linn. in Pobitora Wildlife Sanctuary, Assam, India

    Directory of Open Access Journals (Sweden)

    P. Konwar

    2009-09-01

    Full Text Available Food habits and abundance of food plant species of Rhinoceros unicornis in Pobitora Wildlife Sanctuary were studied from January 1999 through December 2001. Totally 32 numbers of Rhino food plants were identified, of which 15 were grasses, four shrubs, five aquatic hydrophytes and eight tree species (21 terrestrial and 11 aquatic. During the dry season, the Rhino feeds on almost 90% food items from Hemarthria compressa, Arundo donax, Phragmites karka, Cerex rubro-brumee etc. The other short grasses such as Cynodon dactylon, Andropogon ssp., Cenchrus ciliaris, Chrysopogon aciculatus and tender and young shoots and twigs of Schelristechya fuesche, Saccharum spontaneum, Lagerstroemia flosreginae etc. are consumed in limited portions. The rhino consumes 11 cultivated crops and vegetables, viz., Ricinus communis, Oryza sativa, Solanum melongena, Lycopersicon esculentum, Solanum tuberosum, Brassica nigra, Luffa cylindrica, Luffa acutangula, Cucurbita moschata, Cucumis sativus and Ipomoea batatas etc. Highest density of food plant species observed in the study area were Cynodon dactylon (167.5/m2, Hemarthria compressa (73.75/m2, Vetiveria zizanioides (56/m2, Saccharum ravannae (51.5/m2, Pharagmites karka (50.75/m2, Leersia hexandra (46.75/m2, Brachiarea pseudointerrupta (40/m2 and Eichhornia crassipes (35/m2.

  9. Role of delinquent young "orphan" male elephants in high mortality of white rhinoceros in Pilanesberg National Park, South Africa

    Directory of Open Access Journals (Sweden)

    R. Slotow

    2001-07-01

    Full Text Available We describe white rhinoceros (Ceratotherium simum mortality at Pilanesberg National Park, South Africa, focussing on mortality caused by African elephant (Loxodonta africana. We reconstructed records from a range of historical sources, and estimated that up to 49 rhino were killed by elephant. There was confirmed mortality in 1994 and 1996, and based on patterns, we suggest a set of rhino mortality from elephant in 1992. Both sexes and all age classes were victims. There was no significant bias to older animals, but given the rhino population structure, there was a significant bias towards males in adult deaths. The culprits were identified as young male elephants that entered musth about 10 years younger than expected, and maintained musth for a full term at first occurrence. We attributed this to the lack of a mature bull hierarchy in the park, because these elephants were the product of translocation of young animals (<10 years old remaining from culls in Kruger National Park. We emphasise the need for accurate monitoring and record keeping, and a focus on individual identification of key species in small reserves.

  10. New entodiniomorphid ciliates from the intestine of the wild African white rhinoceros belong to a new family, the Gilchristidae.

    Science.gov (United States)

    Ito, Akira; Van Hoven, Wouter; Miyazaki, Yutaka; Imai, Soichi

    2006-11-01

    Gilchristia artemis n.g., n.sp. and Digilchristia draconis n.g., n.sp. in the order Entodiniomorphida are described from the large intestine of the African white rhinoceros, and a new family Gilchristidae is proposed to contain them. These new species have a C-shaped adoral polybrachykinety, a slender vestibular polybrachykinety, and paralabial kineties along the ventral side of the adoral polybrachykinety in their retractable adoral ciliary zone, showing the same arrangement as in the rumen ciliates in the family Ophryoscolecidae. G. artemis has two skeletal plates and D. draconis one plate. In both species the dorsal skeletal plate is bow-shaped, folded in half longitudinally, twisting in the anterior part, and lying along the dorsal left side of the macronucleus. The second plate of G. artemis is slender and lies along the ventral side of the macronucleus. G. artemis has three ciliary arches and D. draconis has four arches along the dorsal and ventral sides of the body. Their arches are long and non-retractable, closely resembling those of ciliates in the families, Spirodiniidae and Cycloposthiidae, and are not analogous to the single retractable ciliary arch of the rumen ciliates in the family Ophryoscolecidae.

  11. The impact of the Krakatoa eruption in 1883 on the population of Rhinoceros sondaicus in Ujung Kulon, with details of rhino observations from 1857 to 1949

    OpenAIRE

    N.J.van. Strien; K. Rookmaaker

    2010-01-01

    A recent suggestion that the entire population of the Javan Rhinoceros in Ujung Kulon National Park was annihilated by the effects of the eruption of the Krakatoa in 1883 is investigated. Based on a review of contemporary reports, it is shown that people survived the waves and remained settled in one village until 1906 when it was evacuated during a plague of tigers. The first report of a rhinoceros in the peninsula of Ujung Kulon dates from 1857 and the animals were occasionally reported fr...

  12. Activation of southern white rhinoceros (Ceratotherium simum simum) estrogen receptors by phytoestrogens: potential role in the reproductive failure of captive-born females?

    Science.gov (United States)

    Tubbs, Christopher; Hartig, Phillip; Cardon, Mary; Varga, Nicole; Milnes, Matthew

    2012-03-01

    The captive southern white rhinoceros (SWR; Ceratotherium simum simum) population serves as an important genetic reservoir critical to the conservation of this vulnerable species. Unfortunately, captive populations are declining due to the poor reproductive success of captive-born females. Captive female SWR exhibit reproductive problems suggested to result from continual ovarian follicular activity and prolonged exposure to endogenous estrogen. However, we investigated the potential role of exogenous dietary phytoestrogens in the reproductive failure of SWR by cloning and characterizing in vitro phytoestrogen binding and activation of recombinant SWR estrogen receptors (ESR). We compared those characteristics with recombinant greater one-horned rhinoceros (GOHR; Rhinoceros unicornis) ESR, a species that receives similar captive diets yet reproduces relatively well. Our results indicate that phytoestrogens bind rhino ESR in a manner similar to other vertebrate species, but there are no differences found in phytoestrogen binding affinity of SWR ESR compared with GOHR ESR. However, species-specific differences in ESR activation by phytoestrogens were detected. The phytoestrogen coumestrol stimulated greater maximal activation of SWR ESR1 than GOHR ESR1. SWR ESR2 were also more sensitive to phytoestrogens and were activated to a greater extent by both coumestrol and daidzein. The concentrations in which significant differences in ESR activation occurred (10(-7) to 10(-5) m) are consistent with circulating concentrations measured in other vertebrate species. Taken together, these findings suggest that phytoestrogens potentially pose a risk to the reproductive health of captive SWR. However, additional studies are needed to further clarify the physiological role of dietary phytoestrogens in the reduced fertility of this species.

  13. Sex-biased inbreeding effects on reproductive success and home range size of the critically endangered black rhinoceros.

    Science.gov (United States)

    Cain, Bradley; Wandera, Antony B; Shawcross, Susan G; Edwin Harris, W; Stevens-Wood, Barry; Kemp, Stephen J; Okita-Ouma, Benson; Watts, Phillip C

    2014-04-01

    A central premise of conservation biology is that small populations suffer reduced viability through loss of genetic diversity and inbreeding. However, there is little evidence that variation in inbreeding impacts individual reproductive success within remnant populations of threatened taxa, largely due to problems associated with obtaining comprehensive pedigree information to estimate inbreeding. In the critically endangered black rhinoceros, a species that experienced severe demographic reductions, we used model selection to identify factors associated with variation in reproductive success (number of offspring). Factors examined as predictors of reproductive success were age, home range size, number of nearby mates, reserve location, and multilocus heterozygosity (a proxy for inbreeding). Multilocus heterozygosity predicted male reproductive success (p58%) and correlated with male home range size (p 44%). Such effects were not apparent in females, where reproductive success was determined by age (p < 0.01, explained deviance 34%) as females raise calves alone and choose between, rather than compete for, mates. This first report of a 3-way association between an individual male's heterozygosity, reproductive output, and territory size in a large vertebrate is consistent with an asymmetry in the level of intrasexual competition and highlights the relevance of sex-biased inbreeding for the management of many conservation-priority species. Our results contrast with the idea that wild populations of threatened taxa may possess some inherent difference from most nonthreatened populations that necessitates the use of detailed pedigrees to study inbreeding effects. Despite substantial variance in male reproductive success, the increased fitness of more heterozygous males limits the loss of heterozygosity. Understanding how individual differences in genetic diversity mediate the outcome of intrasexual competition will be essential for effective management, particularly

  14. Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae).

    Science.gov (United States)

    Bang, Byung-Ho; Rhee, Moon-Soo; Chang, Dong-Ho; Park, Doo-Sang; Kim, Byoung-Chan

    2015-02-01

    A novel, Gram-stain positive, facultative anaerobic, non-motile and straight to curve rod shaped bacterium, strain LV19(T) was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus, which was collected from Yeong-dong, Chuncheongbuk-do, South Korea. The colonies of the new isolate were convex, circular, cream white in color and 1-2 mm in diameter after 3 days incubation on Tryptic Soy Agar at 37 °C. Based on the 16S rRNA gene sequence similarity, the new isolate was most closely related to Erysipelothrix inopinata MF-EP02(T), E. rhusiopathiae ATCC 19414 (T) and E. tonsillarum T-305(T) (94.8, 93.8 and 93.7 % similarity, respectively). Strain LV19(T) grew optimally at 37 °C, at pH 8.0 and in the presence of 0.5 % (w/v) NaCl. Oxidase activity and catalase activity were negative. The major cellular fatty acids (>10 %) were C18:2 cis-9,12 (28.9 %), C18:1 cis-9 (22.3 %), C16:0 (22.2 %) and C18:0 (18.5 %). The cell-wall hydrolysates contained ribose as a major sugar. Major polar lipids were phosphatidylglycerol and three unidentified glycolipids. No quinone was detected. The G+C content of the genomic DNA was 36.3 mol%. The levels of DNA-DNA relatedness between strain LV19(T) and all the reference strains were less than 20 %. On the basis of polyphasic evidence from this study, the isolate is considered to represent a novel species of the genus Erysipelothrix, for which the name Erysipelothrix larvae sp. nov. is proposed; the type strain is LV19(T) (=KCTC 33523(T) = DSM 28480(T)).

  15. An internationally standardized species identification test for use on suspected seized rhinoceros horn in the illegal wildlife trade.

    Science.gov (United States)

    Ewart, Kyle M; Frankham, Greta J; McEwing, Ross; Webster, Lucy M I; Ciavaglia, Sherryn A; Linacre, Adrian M T; The, Dang Tat; Ovouthan, Kanitia; Johnson, Rebecca N

    2018-01-01

    Rhinoceros (rhino) numbers have dwindled substantially over the past century. As a result, three of the five species are now considered to be critically endangered, one species is vulnerable and one species is near-threatened. Poaching has increased dramatically over the past decade due to a growing demand for rhino horn products, primarily in Asia. Improved wildlife forensic techniques, such as validated tests for species identification of seized horns, are critical to aid current enforcement and prosecution efforts and provide a deterrent to future rhino horn trafficking. Here, we present an internationally standardized species identification test based on a 230 base pair cytochrome-b region. This test improves on previous nested PCR protocols and can be used for the discrimination of samples with rhino horn' substitute, but to exclude human DNA, a common contaminant. Phylogenetic analyses using this partial cytochrome-b region resolved the five extant rhino species. Testing successfully returned a sequence and correct identification for all of the known rhino horn samples and vouchered rhino samples from museum and zoo collections, and provided species level identification for 47 out of 52 unknown samples from seizures. Validation and standardization was carried out across five different laboratories, in four different countries, demonstrating it to be an effective and reproducible test, robust to inter laboratory variation in equipment and consumables (such as PCR reagents). This is one of the first species identification tests to be internationally standardized to produce data for evidential proceedings and the first published validated test for rhinos, one of the flagship species groups of the illegal wildlife trade and for which forensic tools are urgently required. This study serves as a model for how species identification tests should be standardized and disseminated for wildlife forensic testing. Copyright © 2017 The Authors. Published by Elsevier B

  16. A numerical and theoretical study on the aerodynamics of a rhinoceros beetle (Trypoxlyus dichotomus) and optimization of its wing kinematics in hover

    Science.gov (United States)

    Oh, Sehyeong; Lee, Boogeon; Park, Hyungmin; Choi, Haecheon

    2017-11-01

    We investigate a hovering rhinoceros beetle using numerical simulation and blade element theory. Numerical simulations are performed using an immersed boundary method. In the simulation, the hindwings are modeled as a rigid flat plate, and three-dimensionally scanned elytra and body are used. The results of simulation indicate that the lift force generated by the hindwings alone is sufficient to support the weight, and the elytra generate negligible lift force. Considering the hindwings only, we present a blade element model based on quasi-steady assumptions to identify the mechanisms of aerodynamic force generation and power expenditure in the hovering flight of a rhinoceros beetle. We show that the results from the present blade element model are in excellent agreement with numerical ones. Based on the current blade element model, we find the optimal wing kinematics minimizing the aerodynamic power requirement using a hybrid optimization algorithm combining a clustering genetic algorithm with a gradient-based optimizer. We show that the optimal wing kinematics reduce the aerodynamic power consumption, generating enough lift force to support the weight. This research was supported by a Grant to Bio-Mimetic Robot Research Center Funded by Defense Acquisition Program Administration, and by Agency for Defense Development (UD130070ID) and NRF-2016R1E1A1A02921549 of the MSIP of Korea.

  17. Rhinos in the Parks: An Island-Wide Survey of the Last Wild Population of the Sumatran Rhinoceros.

    Directory of Open Access Journals (Sweden)

    Wulan Pusparini

    Full Text Available In the 200 years since the Sumatran rhinoceros was first scientifically described (Fisher 1814, the range of the species has contracted from a broad region in Southeast Asia to three areas on the island of Sumatra and one in Kalimantan, Indonesia. Assessing population and spatial distribution of this very rare species is challenging because of their elusiveness and very low population number. Using an occupancy model with spatial dependency, we assessed the fraction of the total landscape occupied by Sumatran rhinos over a 30,345-km2 survey area and the effects of covariates in the areas where they are known to occur. In the Leuser Landscape (surveyed in 2007, the model averaging result of conditional occupancy estimate was ψ(SE[ψ] = 0.151(0.109 or 2,371.47 km2, and the model averaging result of replicated level detection probability p(SE[p] = 0.252(0.267; in Way Kambas National Park--2008: ψ(SE[ψ] = 0.468(0.165 or 634.18 km2, and p(SE[p] = 0.138(0.571; and in Bukit Barisan Selatan National Park--2010: ψ(SE[ψ] = 0.322(0.049 or 819.67 km2, and p(SE[p] = 0.365(0.42. In the Leuser Landscape, rhino occurrence was positively associated with primary dry land forest and rivers, and negatively associated with the presence of a road. In Way Kambas, occurrence was negatively associated with the presence of a road. In Bukit Barisan Selatan, occurrence was negatively associated with presence of primary dryland forest and rivers. Using the probabilities of site occupancy, we developed spatially explicit maps that can be used to outline intensive protection zones for in-situ conservation efforts, and provide a detailed assessment of conserving Sumatran rhinos in the wild. We summarize our core recommendation in four points: consolidate small population, strong protection, determine the percentage of breeding females, and recognize the cost of doing nothing. To reduce the probability of poaching, here we present only the randomized location of site level

  18. Characterization of growth and Oryctes rhinoceros nudivirus production in attached cultures of the DSIR-HA-1179 coleopteran insect cell line.

    Science.gov (United States)

    Pushparajan, Charlotte; Claus, Juan Daniel; Marshall, Sean David Goldie; Visnovsky, Gabriel

    2013-12-01

    The DSIR-HA-1179 coleopteran cell line is a susceptible and permissive host to the Oryctes rhinoceros nudivirus (OrNV), which has been used as a biocontrol agent against the coconut rhinoceros beetle (Oryctes rhinoceros); a pest of palms in the Asia-Pacific region. However, little is known about growth and metabolism of this cell line, knowledge of which is necessary to develop an in vitro large-scale OrNV production process. The strong anchorage-dependent characteristics of the cell line, its particular fragility and its tendency to form dense clumps when manipulated, are the most likely reasons that have precluded further development of the cell line. In order to characterize DSIR-HA-1179 cells, there was first a need for a reliable technique to count the cells. A homogenous cell suspension suitable for enumeration could be produced by treatment with TrypLE Express™ with optimum mean time for cell release calculated as 30 min. The cell line was adapted to grow in four serum-supplemented culture media namely TC-100, IPL-41, Sf-900 II and Sf-900 III and cell growth, glucose consumption, lactate and ammonia production were assessed from static-batch cultures. The maximum viable cell density was reached in Sf-900 II (17.9 × 10(5) cells/ml), with the maximum specific growth rate observed in this culture medium as well (0.0074 h(-1)). Higher production of OrNV was observed in IPL-41 and TC-100 (4.1 × 10(7) TCID50/ml) than in cultures infected in Sf-900 III (2.0 × 10(7) TCID50/ml) and Sf-900 II (1.4 × 10(7) TCID50/ml). At the end of the growth period, glucose was completely consumed in cultures grown in TC-100, while remained in excess in the other three culture media. The cell line produced lactate and ammonia to very low levels in the TC-100 culture medium which is a promising aspect for its cultivation at large-scale.

  19. Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes and Beauveria bassiana (Ascomycota: Hypocreales isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

    Directory of Open Access Journals (Sweden)

    M.W. Khudhair

    2015-12-01

    Full Text Available Virulence of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were tested against Arabian Rhinoceros Beetle, Oryctes agamemnon arabicus larvae. Four concentrations (1×105, 1×107, 1×109 and 1×1011 conidia/mL–1 of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, Beauveria bassiana scored higher mortality rate in short time especially at the concentration of 1×1011 conidia/ mL–1 with lethal time (LT50 12.75 and LT90 20.00; while, Metarhizium anisopliae caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

  20. Polyvalent horse F(Ab`) 2 snake antivenom: Development of ...

    African Journals Online (AJOL)

    A method to obtain polyvalent anti-Bitis and polyvalent-anti-Naja antibodies was developed by immunizing horses with B. arietans, B. nasicornis, B. rhinoceros, N. melanoleuca and N. mossambica crude venoms. Antibody production was followed by the ELISA method during the immunization procedure. Once the desired ...

  1. Comparison of butorphanol-detomidine versus butorphanol-azaperone for the standing sedation of captive greater one-horned rhinoceroses (Rhinoceros unicornis).

    Science.gov (United States)

    Bapodra, Priya; Cracknell, Jonathan; Wolfe, Barbara A

    2014-03-01

    Three adult and two subadult greater one-horned rhinoceroses (Rhinoceros unicornis) were sedated a total of nine times using two different intramuscular sedative combinations in order to compare the effectiveness of these combinations in inducing consistent standing sedation in this species. The sedation protocols compared were butorphanol tartrate (50-60 mg) and detomidine hydrochloride (20-30 mg; BD) versus butorphanol tartrate (80-120 mg) and azaperone (80-120 mg; BA). Specific doses were adjusted according to age and sex class, and based on previous experience. Parameters compared included time to achieve defined levels of sedation, time to recovery following antagonism, physiological parameters including heart rate, respiratory rate, indirect arterial blood pressure, and venous blood gas values. A hydraulic restraint chute was utilized to mechanically restrain animals during the procedures, and blood collection and ophthalmic examinations were conducted on all animals. Both protocols resulted in standing sedation for > or = 22.3 +/- 2.9 min or until antagonists were administered. The BD protocol resulted in deeper and more consistent sedation, compared to the BA protocol. Naltrexone hydrochloride (250-300 mg) and tolazoline hydrochloride (1,500-2,000 mg) were administered intramuscularly to antagonize protocol BD, whereas naltrexone alone (200-500 mg) was used to antagonize BA. Time to full antagonism, defined as normal mentation and ambulation following administration of antagonists, was prolonged in the BD protocol (132.3 +/- 17.2 min) compared with the BA protocol (7.5 +/- 2.5 min). Venous blood gas analysis did not reveal any significant blood gas deviations during sedation when compared with either conscious equine or white rhinoceros (Ceratotherium simum) venous reference ranges. In summary, both combinations resulted in adequate standing sedation for minimally invasive procedures, although BD resulted in more profound and consistent sedation.

  2. Zakaj in kako biti družbeno odgovoren?

    Directory of Open Access Journals (Sweden)

    Milan Simončič

    2015-09-01

    Full Text Available Research Question (RQ: Modern society is critical more and more to social irresponsible actions. Organizations are aware of that fact, in the strategy of its operations want to include the implementation of socially responsibleprinciples. We are interested in how various authors perceive the concept of social responsibility of organizations, what they are benefits of socially responsible organization, how organizations implement its mission, including itsexternal stakeholders involvement and how that activities affects the diversity of cultural environments. Purpose: Study and summary a portion of the available references, in which the authors for different purposes research the social responsibility of organizations. We confronted and compareddifferent viewpoints. Method: We used the method of description (description of facts and method of compilation (summary of known results of scientific research. Results: The collected references are categorized by selected areas presented the classification of the authors, which address particular areas of social responsibility, the resources classified by years of posts and articles by scientific journals. Organization: Organizations will summarize the findings and use it as a starting point for further research, evaluation and eventual optimization of existing models of the concept of corporate social responsibility. Society: Findings will be used as a keystone for further research, society will be aware of the importance of implementing the principles of social responsibility, they will meet the challenges of coexistence with the social responsible organizations. Originality: Groups, which will use the results for further empirical research, are the wider society and organizations who want to improve their image and carry out socially responsible actions. Review of articles highlights various views, which in other articles did not found. Limitations/Future Research: Review of articles was limited to some journals and includes only some of the areas of importance for understanding the problems of deployment and implementation, and the motives for corporate social responsibility. Articles, order than 15 years,not included due to lack of interest in the present circumstances. In further research it would be welcome to increase the number of analyzed articles and take focus to some more specific areas (for instance: consider implementing social responsibility of specific organizations, such as nuclear power plants, chemical industry, evaluate possible further ethical dilemmas in health care, genetic research, cybernetics, etc.

  3. Berg adder ( Bitis atropos ): An unusual case of acute poisoning ...

    African Journals Online (AJOL)

    Abstract. A 5-year-old boy presented to hospital with mild local cytotoxic and severe neurotoxic symptoms. The neurotoxic symptoms included ptosis, fixed dilated pupils and flaccid paralysis with respiratory failure. Mild hyponatraemia was also a clinical feature. After various unsuccessful treatment options were followed, the ...

  4. İlkokuma Yazma Öğretimi Sürecinde Bitişik Eğik Yazı Ve Dik Temel Harflerle Yapılan Eğitimin Öğretmen Görüşlerine Göre Karşılaştırılması A Comparison Of Cursive Italic Writing And Basic Vertical Letters While Teaching Basal Reading And Writing Based On Teachers’ Views

    Directory of Open Access Journals (Sweden)

    Fatma SUSAR KIRMIZI

    2013-09-01

    Full Text Available This study aimed to identify teachers’ views with regard toteaching basal reading and writing with cursive italic writing and basicvertical letters. Action research that is one of the qualitative researchmethods was employed. The population of the study was consisted ofelementary 1st and 2nd graders’ teachers working in Denizli and itsdistricts. Using criterion sampling, 68 elementary teachers were askedfor their opinions. As for the data gathering instrument a survey ofopen-ended six questions developed by the researchers were used in thestudy. Open-coding method was used for data analysis. As a result ofthe analysis, it was seen that the teachers found teaching with basicvertical letters easier both in preparing students for reading and writingand in teaching writing. Furthermore, they argued that with basicvertical letters, students write more beautiful and legible. Most of theteachers participated in the study preferred basic vertical letters justbecause they found cursive italic writing more difficult in teaching basalreading and writing. A comprehensive training related to cursive italicwriting should be provided to teachers. Wider scale studies focusing onwhether teachers prefer cursive italic writing or basic vertical letters inteaching basal reading and writing. Bu araştırma ile öğretmenlerin ilkokuma yazma öğretimi sürecinde bitişik eğik yazı ve dik temel harflerle yapılan eğitim hakkındaki görüşlerinin belirlenmesi amaçlanmıştır. Çalışma sürecinde nitel araştırma yöntemlerinden olan durum çalışması deseni benimsenmiştir. Çalışmanın evreni Denizli il ve ilçelerindeki ilkokullarda görev yapan 1. ve 2. sınıf öğretmenlerinden oluşmaktadır. Araştırmada ölçüt örnekleme yöntemi ile belirlenen 63 kişiden oluşan 1. ve 2. sınıf öğretmeninin görüşüne başvurulmuştur. Veri toplama aracı olarak araştırmacılar tarafından geliştirilen kişisel bilgiler formu ve 6 açık u

  5. Structure of white rhinoceros (Ceratotherium simum) horn investigated by X-ray computed tomography and histology with implications for growth and external form.

    Science.gov (United States)

    Hieronymus, Tobin L; Witmer, Lawrence M; Ridgely, Ryan C

    2006-10-01

    The nasal and frontal horns of two individuals of Ceratotherium simum were examined by x-ray computed tomography (CT scanning), gross observation of sectioned horn, and light microscopy of histological sections of the horn tissue. CT scans of both sets of horns reveal a periodic banding pattern that is evident upon gross observation of sections as darker bands of tissue. The overlap of these bands in both histological and CT slices suggests the presence of both a photoabsorbent component (melanin) and a radiodense component (calcium phosphate salts, most likely hydroxyapatite or octocalcium phosphate). The distribution of these two components in the horns is hypothesized to contribute to the differential wear patterns that produce the characteristic sweeping conical shape of rhinoceros horn from what otherwise (in the absence of wear and UV exposure) would be cylindrical blocks of constantly growing cornified papillary epidermis. Although extant rhinocerotids are unique in possessing a massive entirely keratinous horn that approximates the functions of keratin-and-bone horns such as those of bovid artiodactyls, the tissue structures that make up the horn are strikingly convergent with other examples of papillary cornified epidermis found in horses, artiodactyls, cetaceans, and birds. Copyright (c) 2006 Wiley-Liss, Inc.

  6. Increased inflammation and decreased insulin sensitivity indicate metabolic disturbances in zoo-managed compared to free-ranging black rhinoceros (Diceros bicornis).

    Science.gov (United States)

    Schook, Mandi W; Wildt, David E; Raghanti, Mary Ann; Wolfe, Barbara A; Dennis, Patricia M

    2015-01-01

    Black rhinoceros (rhinos) living in zoos express a host of unusual disease syndromes that are associated with increased morbidity and mortality, including hemolytic anemia, rhabdomyolysis, hepatopathy and ulcerative skin disease, hypophosphatemia and iron overload. We hypothesized that iron overload is a consequence and indicator of disturbances related to inflammation and insulin/glucose metabolism. The objectives of this study were to: (1) generate the first baseline information on biomarkers of inflammation (tumor necrosis factor alpha [TNFα], serum amyloid A [SAA]), insulin sensitivity (insulin, glucose and proxy calculations of insulin sensitivity), phosphate and iron stores (ferritin) using banked serum from free-ranging black rhinos; and (2) then compare serum biomarkers between zoo-managed (n=86 individuals) and free-ranging (n=120) animals. Enzyme immunoassays were validated for serum and then biomarker levels analyzed using mixed models while controlling for sex, age and year of sample collection. Concentrations of TNFα, SAA, insulin and insulin-to glucose ratio were higher (Prhinos managed in ex situ conditions compared to free-living counterparts. Findings indicate that the captive environment is contributing to increased inflammation and decreased insulin sensitivity in this endangered species. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. The Kinetics of the Humoral and Interferon-Gamma Immune Responses to Experimental Mycobacterium bovis Infection in the White Rhinoceros (Ceratotherium simum

    Directory of Open Access Journals (Sweden)

    Sven D. C. Parsons

    2017-12-01

    Full Text Available Mycobacterium bovis is the cause of tuberculosis (TB in a wide range of species, including white rhinoceroses (Ceratotherium simum. Control of the disease relies on the indirect detection of infection by measuring pathogen-specific responses of the host. These are poorly described in the white rhinoceros and this study aimed to characterize the kinetics of immune responses to M. bovis infection in this species. Three white rhinoceroses were infected with M. bovis and their immune sensitization to this pathogen was measured monthly for 20 months. Cell-mediated immunity was characterized in whole blood samples as the differential release of interferon-gamma in response to bovine purified protein derivative (PPDb and avian PPD (PPDa as well as the release of this cytokine in response to the M. bovis proteins 6 kDa early secretory antigenic target (ESAT-6/10 kDa culture filtrate protein (CFP-10. Humoral immunity was quantified as the occurrence or the magnitude of antibody responses to the proteins ESAT-6/CFP-10, MPB83, MPB83/MPB70, and PPDb. The magnitude and duration of immune reactivity varied between individuals; however, peak responses to these antigens were detected in all animals circa 5–9 months postinfection. Hereafter, they gradually declined to low or undetectable levels. This pattern was associated with limited TB-like pathology at postmortem examination and appeared to reflect the control of M. bovis infection following the development of the adaptive immune response. Measurement of these markers could prove useful for assessing the disease status or treatment of naturally infected animals. Moreover, immune responses identified in this study might be used to detect infection; however, further studies are required to confirm their diagnostic utility.

  8. Sperm sex-sorting and preservation for managing the sex ratio and genetic diversity of the southern white rhinoceros (Ceratotherium simum simum).

    Science.gov (United States)

    O'Brien, J K; Roth, T L; Stoops, M A; Ball, R L; Steinman, K J; Montano, G A; Love, C C; Robeck, T R

    2015-01-01

    White rhinoceros ejaculates (n=9) collected by electroejaculation from four males were shipped (10°C, 12h) to develop procedures for the production of chilled and frozen-thawed sex-sorted spermatozoa of adequate quality for artificial insemination (AI). Of all electroejaculate fractions, 39.7% (31/78) exhibited high quality post-collection (≥70% total motility and membrane integrity) and of those, 54.8% (17/31) presented reduced in vitro quality after transport and were retrospectively determined to exhibit urine-contamination (≥21.0μg creatinine/ml). Of fractions analyzed for creatinine concentration, 69% (44/64) were classified as urine-contaminated. For high quality non-contaminated fractions, in vitro parameters (motility, velocity, membrane, acrosome and DNA integrity) of chilled non-sorted and sorted spermatozoa were well-maintained at 5°C up to 54h post-collection, whereby >70% of post-transport (non-sorted) or post-sort (sorted) values were retained. By 54h post-collection, some motility parameters were higher (P<0.05) for non-sorted spermatozoa (total motility, rapid velocity, average path velocity) whereas all remaining motion parameters as well as membrane, acrosome and DNA integrity were similar between sperm types. In comparison with a straw method, directional freezing resulted in enhanced (P<0.05) motility and velocity of non-sorted and sorted spermatozoa, with comparable overall post-thaw quality between sperm types. High purity enrichment of X-bearing (89±6%) or Y-bearing (86±3%) spermatozoa was achieved using moderate sorting rates (2540±498X-spermatozoa/s; 1800±557Y-spermatozoa/s). Collective in vitro characteristics of sorted-chilled or sorted-frozen-thawed spermatozoa derived from high quality electroejaculates indicate acceptable fertility potential for use in AI. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. The genome of Oryctes rhinoceros nudivirus provides novel insight into the evolution of nuclear arthropod-specific large circular double-stranded DNA viruses.

    Science.gov (United States)

    Wang, Yongjie; Bininda-Emonds, Olaf R P; van Oers, Monique M; Vlak, Just M; Jehle, Johannes A

    2011-06-01

    The Oryctes rhinoceros nudivirus (OrNV) is a dsDNA virus with enveloped, rod-shaped virions. Its genome is 127,615 bp in size and contains 139 predicted protein-coding open reading frames (ORFs). In-depth genome sequence comparisons revealed a varying number of shared gene homologues, not only with other nudiviruses (NVs) and baculoviruses, but also with other arthropod-specific large dsDNA viruses, including the so-called Monodon baculovirus (MBV), the salivary gland hypertrophy viruses (SGHVs) and white spot syndrome virus (WSSV). Nudivirus genomes contain 20 baculovirus core gene homologues associated with transcription (p47, lef-8, lef-9, lef-4, vlf-1, and lef-5), replication (dnapol and helicase), virus structure (p74, pif-1, pif-2, pif-3, 19kda/pif-4, odv-e56/pif-5, vp91, vp39, and 38K), and unknown functions (ac68, ac81, and p33). Most strikingly, a set of homologous genes involved in peroral infection (p74, pif-1, pif-2, and pif-3) are common to baculoviruses, nudiviruses, SGHVs, and WSSV indicating an ancestral mode of infection in these highly diverged viruses. A gene similar to polyhedrin/granulin encoding the baculovirus occlusion body protein was identified in non-occluded NVs and in Musca domestica SGHV evoking the question of the evolutionary origin of the baculovirus polyhedrin/granulin gene. Based on gene homologies, we further propose that the shrimp MBV is an occluded member of the nudiviruses. We conclude that baculoviruses, NVs and the shrimp MBV, the SGHVs and WSSV share the significant number of conserved genetic functions, which may point to a common ancestry of these viruses.

  10. The Kinetics of the Humoral and Interferon-Gamma Immune Responses to ExperimentalMycobacterium bovisInfection in the White Rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Parsons, Sven D C; Morar-Leather, Darshana; Buss, Peter; Hofmeyr, Jennifer; McFadyen, Ross; Rutten, Victor P M G; van Helden, Paul D; Miller, Michele A; Michel, Anita Luise

    2017-01-01

    Mycobacterium bovis is the cause of tuberculosis (TB) in a wide range of species, including white rhinoceroses ( Ceratotherium simum ). Control of the disease relies on the indirect detection of infection by measuring pathogen-specific responses of the host. These are poorly described in the white rhinoceros and this study aimed to characterize the kinetics of immune responses to M. bovis infection in this species. Three white rhinoceroses were infected with M. bovis and their immune sensitization to this pathogen was measured monthly for 20 months. Cell-mediated immunity was characterized in whole blood samples as the differential release of interferon-gamma in response to bovine purified protein derivative (PPDb) and avian PPD (PPDa) as well as the release of this cytokine in response to the M. bovis proteins 6 kDa early secretory antigenic target (ESAT-6)/10 kDa culture filtrate protein (CFP-10). Humoral immunity was quantified as the occurrence or the magnitude of antibody responses to the proteins ESAT-6/CFP-10, MPB83, MPB83/MPB70, and PPDb. The magnitude and duration of immune reactivity varied between individuals; however, peak responses to these antigens were detected in all animals circa 5-9 months postinfection. Hereafter, they gradually declined to low or undetectable levels. This pattern was associated with limited TB-like pathology at postmortem examination and appeared to reflect the control of M. bovis infection following the development of the adaptive immune response. Measurement of these markers could prove useful for assessing the disease status or treatment of naturally infected animals. Moreover, immune responses identified in this study might be used to detect infection; however, further studies are required to confirm their diagnostic utility.

  11. Recycling of Badger/Fox Burrows in Late Pleistocene Loess by Hyenas at the Den Site Bad Wildungen-Biedensteg (NW, Germany: Woolly Rhinoceros Killers and Scavengers in a Mammoth Steppe Environment of Europe

    Directory of Open Access Journals (Sweden)

    Cajus Diedrich

    2013-01-01

    Full Text Available The Late Pleistocene (MIS 5c-d Ice Age spotted hyena open air den and bone accumulation site Bad Wildungen-Biedensteg (Hesse, NW, Germany represents the first open air loess fox/badger den site in Europe, which must have been recycled by Crocuta crocuta spelaea (Goldfuss, 1823 as a birthing den. Badger and fox remains, plus remains of their prey (mainly hare, have been found within the loess. Hyena remains from that site include parts of cub skeletons which represent 10% of the megafauna bones. Also a commuting den area existed, which was well marked by hyena faecal pellets. Most of the hyena prey bones expose crack, bite, and nibbling marks, especially the most common bones, the woolly rhinoceros Coelodonta antiquitatis (NISP  =  32%. The large amount of woolly rhinoceros bones indicate hunting/scavenging specializing on this large prey by hyenas. Other important mammoth steppe hyena prey remains are from Mammuthus primigenius, Equus caballus przewalskii, Bison/Bos, Megaloceros giganteus, Cervus elaphus, and Rangifer tarandus. The few damaged bone remains of a scavenged cave bear Ursus spelaeus subsp. are unique for an open air situation. Abundant micromammal, frog, and some fish remains were concentrated in “pellets” that contain mainly mammoth steppe micromammals and also frog and fish remains that seem to originate from the nearby river/lake.

  12. A study on the behavior and colonization of translocated Greater One-horned Rhinos Rhinoceros unicornis (Mammalia: Perissodactyla: Rhinocerotidae during 90 days from their release at Manas National Park, Assam India

    Directory of Open Access Journals (Sweden)

    Deba Kumar Dutta

    2015-02-01

    Full Text Available Under the Indian Rhino Vision 2020, 18 wild Greater One-horned Rhinos Rhinoceros unicornis were translocated from two rhino bearing areas, Kaziranga National Park and Pobitora Wildlife Sanctuary to Manas National Park within the state of Assam, from 2008 to 2012. Following the release, the rhinos were closely monitored through radio tracking and direct observation to record their colonization pattern and behavioral adaptation to the new environment. Out of the 18 rhinos released at Manas, 16 (89% rhinos dispersed in approximately the same direction from the release sites. The rhinos released from Pobitora Wildlife Sanctuary strayed more when compared to the rhinos from Kaziranga National Park. This paper describes the behavior patterns observed in Manas National Park, which may provide a useful alternative approach for future rhino translocation.

  13. Amputation for a puff adder (Bitis arietans) envenomation in a child ...

    African Journals Online (AJOL)

    inhibiting, pro-thrombotic, vasodilating, cardiodepressant, and neurotoxic effects. [3-8]. When envenomation occurs in a human tissue necrosis, coagulopathy, hypotension, thrombocytopenia and spontaneous bleeding have all been reported [9].

  14. TERRITORIALITY: THE EXAMPLE OF THE WHITE RHINOCEROS ...

    African Journals Online (AJOL)

    winter-feeding territories, group-defended territories, and even roosting territories (Nice 1941). Many possible advantageous .... reproduction: (i) calves; (ii) adolescents; (iii) cows; (iv) territorial bulls ; (v) su bsidiary bulls. ... cow which has lost her calf will, however, accept the company of one or more adolescents, and the.

  15. Taxonomy Icon Data: white rhinoceros [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available g http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Ceratotherium+simum&t=L http://biosciencedbc.jp/taxonomy_...icon/icon.cgi?i=Ceratotherium+simum&t=NL http://biosciencedbc.jp/taxonomy_icon/ic...on.cgi?i=Ceratotherium+simum&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Ceratotherium+simum&t=NS ...

  16. Skin lipid structure controls water permeability in snake molts.

    Science.gov (United States)

    Torri, Cristian; Mangoni, Alfonso; Teta, Roberta; Fattorusso, Ernesto; Alibardi, Lorenzo; Fermani, Simona; Bonacini, Irene; Gazzano, Massimo; Burghammer, Manfred; Fabbri, Daniele; Falini, Giuseppe

    2014-01-01

    The role of lipids in controlling water exchange is fundamentally a matter of molecular organization. In the present study we have observed that in snake molt the water permeability drastically varies among species living in different climates and habitats. The analysis of molts from four snake species: tiger snake, Notechis scutatus, gabon viper, Bitis gabonica, rattle snake, Crotalus atrox, and grass snake, Natrix natrix, revealed correlations between the molecular composition and the structural organization of the lipid-rich mesos layer with control in water exchange as a function of temperature. It was discovered, merging data from micro-diffraction and micro-spectroscopy with those from thermal, NMR and chromatographic analyses, that this control is generated from a sophisticated structural organization that changes size and phase distribution of crystalline domains of specific lipid molecules as a function of temperature. Thus, the results of this research on four snake species suggest that in snake skins different structured lipid layers have evolved and adapted to different climates. Moreover, these lipid structures can protect, "safety", the snakes from water lost even at temperatures higher than those of their usual habitat. Copyright © 2013 Elsevier Inc. All rights reserved.

  17. Upajmo si biti starši. Praktično delovanje dveh sol za starše

    Directory of Open Access Journals (Sweden)

    Andreja Jereb

    2005-12-01

    Full Text Available V prispevku predstavljam dva primera uspdne prakse sole za starSe. Prvi zasluzi pozornost, ker zajema vse potrebne vsebine za ozavdcanje starsev o kompleksnosti in konfliktnosti odnosov v druzini tako med zakoncema kot tudi med starsi in mladostniki. Program je pripravljen sistematicno, sreeanja so strukturirana in privlacna za starSe mladostnikov, ki marsikatero tezavo iz doma prepoznajo v stevilnih primerih iger vlog. Drugi, spanski primer, zasluzi pozornost predvsem zaradi aktivne vloge starsev pri pripravi na sreeanje, z leti vedno aktivnejSe vloge na srecanjih in v pogovorih v druZini, zaradi cesar je precej zahteven tako za voditelja kot za starSe, a sodee po izjavah starsev iskana in uspdna metoda dela s starsi.

  18. Territoriality: the example of the White Rhinoceros | Owen-Smith ...

    African Journals Online (AJOL)

    African Zoology. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 7, No 1 (1972) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Download this PDF file. The PDF file you selected should load here if your ...

  19. Faecal progestagen profiles in wild southern white rhinoceros ...

    African Journals Online (AJOL)

    Knowledge of the reproductive biology of wild animals can provide valuable information for the development of appropriate in situ and ex situ management plans. The present study aimed to establish a non-invasive protocol for monitoring faecal progestagen metabolite (FPM) patterns in wild female southern white ...

  20. Territoriality: the example of the White Rhinoceros | Owen-Smith ...

    African Journals Online (AJOL)

    Territoriality in the white rhino may be characterised as a space-correlated dominance relationship with the function of ordering reproductive competition among males. It plays no significant role in population regulation. These conclusions are extended to other territorial ungulates. Methodological criteria for territoriality are ...

  1. Dietary impact on circulating glucose profiles in the white rhinoceros.

    Science.gov (United States)

    Berkeley, E V; Linklater, W L; Dierenfeld, E S

    2011-04-01

    Excess dietary glucose may be a factor in several captive wildlife diseases and reproductive abnormalities. The first step in understanding the health consequences of diets high in glucose is to characterize how dietary glucose concentrations change circulating glucose profiles. We adapted the glycemic index approach to detect differences in blood glucose concentrations in white rhinos in response to different meals. Six white rhinos were fasted overnight then randomly assigned to be fed 5 kg of grass hay and one of five meals varying in digestible energy (DE) availability and source (10% DE glucose, 5% DE glucose, 10% DE pelleted horse feed, 10% DE lucerne hay, 10% DE grass hay). After eating, the blood glucose response peaked 45-90 min later and remained elevated up to 180 min. Area under the curve results demonstrated that the blood glucose response was not different between diets. However, at 90 min, serum glucose levels in rhinos eating the 10% lucerne hay diet were significantly lower than the 5% glucose and 10% glucose diets but not the 10% pellet nor 10% grass hay diets. The changes in blood glucose responses to different diets were similar in magnitude to reported domestic horse profiles but are higher than predicted by allometric scaling. We conclude that the grass hay, lucerne hay and low glycemic index horse pellets fed in this study resulted in similar blood glucose responses in white rhinos. The validation of the methodology used in this study is a first step towards elucidating the relationship between glucose, obesity, health and reproduction in rhinos. © 2010 Blackwell Verlag GmbH.

  2. Sexual maturation in the Sumatran rhinoceros (Dicerorhinus sumatrensis).

    Science.gov (United States)

    Roth, Terri L; Reinhart, Paul R; Romo, John S; Candra, Dedi; Suhaery, Andriansyah; Stoops, Monica A

    2013-01-01

    To help save the Sumatran rhino from extinction, the captive breeding program must capitalize on each rhino's reproductive lifespan. Doing so requires knowing when calves are sexually mature. The goal of this study was to monitor physiological changes associated with sexual maturation in two captive born calves (one male and one female) to determine the approximate age of maturity for both sexes of this species. Fecal testosterone metabolites were monitored in the male calf from 6 months to 7 years of age, and fecal pregnane metabolites were measured in the female calf from 6 months to 5.5 years of age. In addition, rectal ultrasonography was employed to monitor changes in ovarian activity from 2 to 5.5 years of age. The male calf's fecal testosterone concentrations reached levels comparable to those detected in samples from adult males when he was 6-6.5 years of age. The first pre-ovulatory sized follicle was observed on the ovaries of the female calf when she was 4.75 years old, but fecal pregnane metabolite concentrations only reached maximum mean concentrations and variability when she was 5-5.5 years of age. Results from this study indicate that male and female Sumatran rhino calves are sexually mature at 6-6.5 and 5-5.5 years of age, respectively. © 2013 Wiley Periodicals, Inc.

  3. Effects of management strategies on glucocorticoids and behavior in Indian rhinoceros (Rhinoceros unicornis): translocation and operant conditioning.

    Science.gov (United States)

    Capiro, Jonnie M; Stoops, Monica A; Freeman, Elizabeth W; Clawson, Dave; Schook, Mandi W

    2014-01-01

    The ex situ Indian rhino population experienced a decrease in genetic diversity indicating that the breeding program could possibly benefit from novel reproductive management strategies to ensure population sustainability. We sought to determine how management tools used for reproductive management, specifically translocation and operant conditioning, impact physiological and behavioral measures of welfare in Indian rhinos. First, an adrenocorticotropic hormone challenge performed in an adult male resulted in a 38-fold increase in urinary and a 3.5-fold increase in fecal glucocorticoid metabolites (FGM). Mean and peak FGM differed among three females, but all demonstrated elevated (P operant conditioning to stand during transrectal ultrasound exams were monitored and rhinos differed in their mean and peak FGM concentrations. However, FGM were not different before versus during training or on pasture versus in the barn. One female exhibited more stereotypic behavior during training in the barn than on pasture (P operant conditioning only resulted in minimal changes in behaviors and FGM. © 2014 Wiley Periodicals Inc.

  4. Development of Equine IgG Antivenoms against Major Snake Groups in Mozambique.

    Science.gov (United States)

    Guidolin, Felipe Raimondi; Caricati, Celso Pereira; Marcelino, José Roberto; da Silva, Wilmar Dias

    2016-01-01

    Snake envenoming is a significant public health problem in underdeveloped and developing countries. In sub-Saharan Africa, it is estimated that 90,000-400,000 envenomations occur each year, resulting in 3,500-32,000 deaths. Envenomings are caused by snakes from the Viperidae (Bitis spp. and Echis spp.) and Elapidae (Naja spp. and Dendroaspis spp.) families. The African continent has been suffering from a severe antivenom crisis and current antivenom production is only sufficient to treat 25% of snakebite cases. Our aim is to develop high-quality antivenoms against the main snake species found in Mozambique. Adult horses primed with the indicated venoms were divided into 5 groups (B. arietans; B. nasicornis + B. rhinoceros; N. melanoleuca; N. mossambica; N. annulifera + D. polylepis + D. angusticeps) and reimmunized two times for antivenom production. Blood was collected, and plasma was separated and subjected to antibody purification using caprylic acid. Plasmas and antivenoms were subject to titration, affinity determination, cross-recognition assays and in vivo venom lethality neutralization. A commercial anti-Crotalic antivenom was used for comparison. The purified antivenoms exhibited high titers against B. arietans, B. nasicornis and B. rhinoceros (5.18 x 106, 3.60 x 106 and 3.50 x 106 U-E/mL, respectively) and N. melanoleuca, N. mossambica and N. annulifera (7.41 x 106, 3.07 x 106 and 2.60 x 106 U-E/mL, respectively), but lower titers against the D. angusticeps and D. polylepis (1.87 x 106 and 1.67 x 106 U-E/mL). All the groups, except anti-N. melanoleuca, showed significant differences from the anti-Crotalic antivenom (7.55 x 106 U-E/mL). The affinity index of all the groups was high, ranging from 31% to 45%. Cross-recognition assays showed the recognition of proteins with similar molecular weight in the venoms and may indicate the possibility of paraspecific neutralization. The three monospecific antivenoms were able to provide in vivo protection. Our

  5. Tko želi biti medijski pismen? Metode medijskih istraživanja i njihova primjena na koncept “medijske pismenosti”

    OpenAIRE

    Volčić, Zala

    2003-01-01

    The field of media studies deals with issues involving the complex process of consumption of mass media and its relation to audiences' meaning-making practices. One of the enduring concerns in media studies is the ongoing question regarding the power of mass media, their effects, and their roles as vehicles of culture. For example, in some media approaches, mass media are analyzed simply as propaganda machines that have direct media effects. In more sophisticated versions...

  6. Remote assessment of stress in white rhinoceros (Ceratotherium simum) and black rhinoceros (Diceros bicornis) by measurement of adrenal steroids in feces.

    Science.gov (United States)

    Turner, John W; Tolson, Peter; Hamad, Noel

    2002-09-01

    This study monitored fecal cortisol and corticosterone levels in 14 black rhinoceroses (Diceros bicornis) and in seven white rhinoceroses (Certotherium simum) under various conditions of captivity, including translocation. Free cortisol and free corticosterone were measured in methylene chloride extracts of feces, using high-performance liquid chromatography (HPLC). The extraction-assay method was validated for quantitative measurement of these hormones by mass spectroscopy analysis, chemical derivitization, and radiolabel tracking and recovery. Both cortisol and corticosterone were extractable from feces and routinely detectable by HPLC. In three nonstressed, captivity-adapted white rhinoceroses monitored across 21 days of routine activity, fecal cortisol ranged from 2.0 to 7.3 ng/g dry feces and corticosterone from 4.0 to 10.8 ng/g dry feces, with no observable trend. Matched plasma, urine, and fecal samples in these rhinoceroses yielded corticosterone:cortisol ratios of 2.0:1.0, 2.7:1.0, and 2.2:1.0, respectively. Both black rhinoceroses (n = 5) and white rhinoceroses (n = 4) exhibited higher fecal cortisol (6.9- to 10.0-fold) and corticosterone (3.2- to 4.5-fold) levels in association with restraint-translocation than in limited free-roaming conditions. In five black rhinoceroses monitored across 6 wk after release from translocation, fecal levels of both cortisol and corticosterone decreased significantly between week 1 and weeks 4-6. In general, cortisol and corticosterone paralleled each other, with cortisol exhibiting a greater range of response. Measurement of either hormone in feces appears to be reliable for adrenal axis monitoring in the white and the black rhinoceroses.

  7. How healthy are the rhinoceros populations in the Hluhluwe-iMfolosi ...

    African Journals Online (AJOL)

    provided by Kalman filtering. Craig et al. (1997) ... trend in its size over time, as well as the method by which these animals are counted. Bayesian .... (d) Given the estimated ˆε1 and r, successively compute the estimated true population size at each survey as. ˆµ1. = y1 - ˆε1,. ˆµ2. = y2 - ˆε2. = (y1 - ˆε1)ers1 - l1. (ers1 - 1 rs1. ).

  8. Assessment of a portable lactate meter for field use in the white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Cole, Georgina C; Tordiffe, Adrian S W; Steenkamp, Gerhard

    2017-11-10

    Blood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate meter and a laboratory method was assessed, as was the stability of rhino blood lactate in the anticoagulant sodium fluoride/potassium oxalate (fluoride/oxalate). Blood samples were obtained from 53 white rhinos that had been immobilised for management reasons. Lactate was measured by means of a handheld meter using whole blood in heparin (WBHEP), whole blood in fluoride/oxalate (WBFO) and fluoride/oxalate plasma (PFO). Results were recorded in both blood (BL) and plasma (PL) modes and compared to an established laboratory method for measuring plasma lactate. To assess the stability of lactate over time, blood lactate in fluoride/oxalate was measured on the handheld meter at intervals for up to 91 h. Agreement was best using WBFO in PL mode, with small bias (-0.16), tight 95% limits of agreement (LOA) (-1.46, 1.14) and a Pc (95% CI) of 0.97 (0.92, 0.99). The agreement was improved for all sample types when using the PL mode compared to the blood lactate (BL) mode. Blood lactate was stable in fluoride/oxalate for 91 h, with a mean change from baseline of 0.15 (-0.178, 0.478) mmol/L (mean, 95% CI). The handheld meter was found to be suitable for field use in white rhinos but provided more reliable results with the device in PL mode. Furthermore, rhino blood lactate was found to be stable in fluoride/oxalate for as long as 3 days.

  9. Horn growth rates of free-ranging white and black rhinoceros

    Directory of Open Access Journals (Sweden)

    D.J. Pienaar

    1991-09-01

    Full Text Available The intrinsic and observed anterior hom growth of white and black rhinoceroses is discussed. The effect of age and hom rubbing on hom growth is explained. Species and sex related differences in hom size and mass are investigated.

  10. Assessment of a portable lactate meter for field use in the white rhinoceros (Ceratotherium simum

    Directory of Open Access Journals (Sweden)

    Georgina C. Cole

    2017-11-01

    Full Text Available Blood lactate is a predictor of mortality in critically ill humans and animals. Handheld lactate meters have the potential to be used in the field to evaluate the condition of severely injured rhinoceroses but have not been compared with laboratory-based methods. Agreement between a handheld lactate meter and a laboratory method was assessed, as was the stability of rhino blood lactate in the anticoagulant sodium fluoride/potassium oxalate (fluoride/oxalate. Blood samples were obtained from 53 white rhinos that had been immobilised for management reasons. Lactate was measured by means of a handheld meter using whole blood in heparin (WBHEP, whole blood in fluoride/oxalate (WBFO and fluoride/oxalate plasma (PFO. Results were recorded in both blood (BL and plasma (PL modes and compared to an established laboratory method for measuring plasma lactate. To assess the stability of lactate over time, blood lactate in fluoride/oxalate was measured on the handheld meter at intervals for up to 91 h. Agreement was best using WBFO in PL mode, with small bias (-0.16, tight 95% limits of agreement (LOA (-1.46, 1.14 and a Pc (95% CI of 0.97 (0.92, 0.99. The agreement was improved for all sample types when using the PL mode compared to the blood lactate (BL mode. Blood lactate was stable in fluoride/oxalate for 91 h, with a mean change from baseline of 0.15 (-0.178, 0.478 mmol/L (mean, 95% CI. The handheld meter was found to be suitable for field use in white rhinos but provided more reliable results with the device in PL mode. Furthermore, rhino blood lactate was found to be stable in fluoride/oxalate for as long as 3 days.

  11. Inner ventricular structures and valves of the heart in white rhinoceros (Ceratotherium simum).

    Science.gov (United States)

    Erdoğan, Serkan; Lima, Martin; Pérez, William

    2014-01-01

    In this study, we describe the internal structures of both ventricles and the valvular apparatus of the heart of the white rhino. In the right of the heart, three papillary muscles were found in septal and marginal walls and m. papillaris magnus was the biggest. There was only one m. papillaris parvus in the right ventricle. The right atrioventricular valve was tricuspid, and the parietal cusp was longest. In the left of the heart, two papillary muscles were found on the septal wall and the subauricular was the biggest. The left atrioventricular valve was bicuspid and the parietal cusp was longest. There were no nodules in the valves of the pulmonary trunk and aorta, and the semilunar valves had many fibrous folds and transparent parts. Within the cardiac skeleton there was a cartilago cordis which occupied a small part of the right fibrous trigone. While the right ventricle included only one septomarginal trabecula, there were many trabeculae in the left ventricle. In both ventricles, the endocardium was thin and the subendocardial network was visible, also their continuation with the septomarginal trabeculae. We also found many trabeculae carneae in the dorsal part of the ventricles.

  12. Black rhinoceros (Diceros bicornis) populations in northwestern Namibia are apparently not infected with piroplasms.

    Science.gov (United States)

    Penzhorn, Barend L; Oosthuizen, Marinda C; Bosman, Anna-Mari; Kilian, J Werner; Horak, Ivan G

    2008-10-01

    Babesiosis is a potentially fatal disease in black rhinoceroses. Blood specimens collected from black rhinoceroses from Etosha National Park (n = 29) and Damaraland (n = 22), Namibia, were subjected to polymerase chain reaction using Theileria and Babesia genus-specific primers and reverse line blot, with negative results. The animals were sparsely infested with ticks. In the absence of suitable prophylactic measures, naïve rhinoceroses would be at risk if translocated to Babesia-endemic areas.

  13. Extinctions, genetic erosion and conservation options for the black rhinoceros (Diceros bicornis)

    DEFF Research Database (Denmark)

    Moodley, Yoshan; Russo, Isa-Rita M.; Dalton, Desiré L.

    2017-01-01

    populations in countries such as Nigeria, Cameroon, Chad, Eritrea, Ethiopia, Somalia, Mozambique, Malawi and Angola no longer exist. We found that the historic range of the West African subspecies (D. b. longipes), declared extinct in 2011, extends into southern Kenya, where a handful of individuals survive...

  14. Remotely piloted aircraft systems as a rhinoceros anti-poaching tool in Africa

    CSIR Research Space (South Africa)

    Mulero-Pazmany, M

    2014-01-01

    Full Text Available and the agents involved in their protection are demanding new technologies to increase their efficiency in the field. We assessed the use of remotely piloted aircraft systems (RPAS) to monitor for poaching activities. We performed 20 flights with 3 types...

  15. How healthy are the rhinoceros populations in the Hluhluwe-iMfolosi ...

    African Journals Online (AJOL)

    The method is based on a simple state space model which takes into account the (unknown) birth rate in the population and all known losses (mortalities and relocations) and gains (introductions) in the population between successive surveys, as well as the errors in the survey estimates. The method is applied to the White- ...

  16. The rhinoceros among Serpents: Comparative anatomy and experimental biophysics of Calabar burrowing python (Calabaria reinhardtii) skin.

    Science.gov (United States)

    Han, Dawei; Young, Bruce A

    2018-01-01

    The Calabar burrowing python (Calabaria reinhardtii) has a unique combination of marked thickness of the integumentary layers, a highly organized lamellate arrangement of the dermal collagen bundles, and a reduction in the size of the interscale hinge region of the integument. Biomechanical testing demonstrates that the skin of C. reinhardtii is more resistant to penetration than the skin of other snakes. The laminar arrangement of the collagen bundles provides for penetrative resistance, even while maintaining the flexibility characteristic of snake skin. Considering the life history of this species, it is hypothesized that the specialized integument of C. reinhardtii is a passive defensive mechanism against penetrative bites from maternal rodents and predators. © 2017 Wiley Periodicals, Inc.

  17. Suckling behaviour in captive Dendrohyrax arboreus

    African Journals Online (AJOL)

    1980-11-06

    Nov 6, 1980 ... drohyrax dorsalis) Biologia Gabonica I. RUDNAI, J. 1984. Activity cycle and space utilization in a captive. Dendrohyrax arboreus group. S. Afr. J. Zool. 19: 124-128. SALE, J.B. 1 965a. Some aspects of the behaviour and ecology of the rock hyraces (Genera Procavia and Heterohyrax) Ph.D. thesis, Ju·.

  18. Tooth wear in captive rhinoceroses (Diceros, Rhinoceros, Ceratotherium: Perissodactyla) differs from that of free-ranging conspecifics

    NARCIS (Netherlands)

    Taylor, L.A.; Müller, D.W.H.; Schwitzer, C.; Kaiser, T.M.; Codron, D.; Schulz, E.; Clauss, M.

    2014-01-01

    Tooth wear can affect body condition, reproductive success and life expectancy. Poor dental health is frequently reported in the zoo literature, and abrasion-dominated tooth wear, which is typical for grazers, has been reported in captive browsing ruminants. The aim of this study was to test if a

  19. Unusual mortalities of the eastern black rhinoceros (Diceros bicornis michaeli) due to clostridial enterotoxaemia in Ol Jogi Pyramid Sanctuary, Kenya

    NARCIS (Netherlands)

    Ndeereh, D.; Ouma, B.O.; Gaymer, J.; Mutinda, M.; Gakuya, F.

    2012-01-01

    Nine eastern black rhinoceroses (Diceros bicornis michaeli) developed clinical clostridial enterotoxaemia between May and July 2010 in the Pyramid Black Rhino Sanctuary within the Ol Jogi Conservancy, Laikipia, Kenya. The rhinos presented with a peracute syndrome characterised by severe abdominal

  20. Status Kritis Dua Jenis Badak di Indonesia

    OpenAIRE

    Haerudin R. Sadjudin; Mochammad Syamsudin; Widodo Sukohadi Ramono

    2016-01-01

    There are five species of rhinoceros in the world, two types of African (Diceros bicornis and Ceratotherium simum) and three species in Asia (Rhinoceros unicornis, Rhinoceros sondaicus and Dicerorhinus sumatrensis). Indonesia has two types, namely: the Javan rhino (Rhinoceros sondaicus Desmarest, 1822) and the Sumatran rhino (Dicerorhinus sumatrensis Fischer, 1814).Both types of rhino in Indonesia is not endemic wildlife, but it is a species relich; formerly spread is very wide, but now in ad...

  1. Buphagus erythrorhynchus

    African Journals Online (AJOL)

    for giraffe, black rhinoceros, roan antelope and sable antelope Hippotragus niger (Stutterheim 1979), whereas in the Hluhluwe-Umfolozi Game Reserve complex it associates with seven species, preferring buffalo, black rhinoceros and white rhinoceros (Stutterheim 1980), and in the Mkuzi Game Reserve and Nxwala State ...

  2. ANALIZA SODNIH ODLOČITEV O (NEPOŠTENIH) SPLOŠNIH POGOJIH POSLOVANJA

    OpenAIRE

    Podlesnik, Polona

    2017-01-01

    Splošni pogoji poslovanja (pogodbe) stranko zavezujejo, če so ji bili znani ali bi ji morali biti znani, pogoj pa je, da so objavljeni na običajen način. V potrošniških pogodbah se pravni standard običajne objave presoja strožje, v primeru zavarovalne pogodbe pa je izpolnjen- splošni pogoji morajo biti zavarovancu izročeni. Splošni pogoji morajo biti v pogodbenem jeziku ali takem, ki ga nasprotna stranka razume, veljajo pa tudi v drugem jeziku, če jih je nasprotna stranka v medsebojni praksi ...

  3. Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros (Diceros bicornis)

    Science.gov (United States)

    Nijhof, Ard M.; Penzhorn, Banie L.; Lynen, Godelieve; Mollel, Johnson O.; Morkel, Pete; Bekker, Cornelis P. J.; Jongejan, Frans

    2003-01-01

    A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov. PMID:12734294

  4. Rhinoceros feet step out of a rule-of-thumb: a wildlife imaging pioneering approach of synchronized computed tomography-digital radiography.

    Directory of Open Access Journals (Sweden)

    Gabriela Galateanu

    Full Text Available Currently, radiography is the only imaging technique used to diagnose bone pathology in wild animals situated under "field conditions". Nevertheless, while chronic foot disease in captive mega-herbivores is widely reported, foot radiographic imaging is confronted with scarcity of studies. Numerous hindrances lead to such limited numbers and it became very clear that the traditional perspective on bone imaging in domestic animals based on extensive studies and elaborated statistical evaluations cannot be extrapolated to their non-domestic relatives. For these reasons, the authors initiated a multi-modality imaging study and established a pioneering approach of synchronized computed tomography (CT and digital radiography (DR, based on X-ray projections derived from three-dimensional CT reconstructed images. Whereas this approach can be applied in any clinical field, as a case of outstanding importance and great concern for zoological institutions, we selected foot bone pathologies in captive rhinoceroses to demonstrate the manifold applications of the method. Several advances were achieved, endowing the wildlife clinician with all-important tools: prototype DR exposure protocols and a modus operandi for foot positioning, advancing both traditional projections and, for the first-time, species-related radiographic views; assessment of radiographic diagnostic value for the whole foot and, in premiere, for each autopodial bone; together with additional insights into radiographic appearance of bone anatomy and pathology with a unique, simultaneous CT-DR correlation. Based on its main advantages in availing a wide range of keystone data in wildlife imaging from a limited number of examined subjects and combining advantages of CT as the golden standard method for bone diseases' diagnostic with DR's clinical feasibility under field conditions, synchronized CT-DR presents a new perspective on wildlife's health management. With this we hope to provide veterinary clinicians with concrete imaging techniques and substantial diagnostic tools, which facilitate straightforward attainment and interpretation of field radiography images taken worldwide.

  5. Rhinoceros feet step out of a rule-of-thumb: a wildlife imaging pioneering approach of synchronized computed tomography-digital radiography.

    Science.gov (United States)

    Galateanu, Gabriela; Hermes, Robert; Saragusty, Joseph; Göritz, Frank; Potier, Romain; Mulot, Baptiste; Maillot, Alexis; Etienne, Pascal; Bernardino, Rui; Fernandes, Teresa; Mews, Jurgen; Hildebrandt, Thomas Bernd

    2014-01-01

    Currently, radiography is the only imaging technique used to diagnose bone pathology in wild animals situated under "field conditions". Nevertheless, while chronic foot disease in captive mega-herbivores is widely reported, foot radiographic imaging is confronted with scarcity of studies. Numerous hindrances lead to such limited numbers and it became very clear that the traditional perspective on bone imaging in domestic animals based on extensive studies and elaborated statistical evaluations cannot be extrapolated to their non-domestic relatives. For these reasons, the authors initiated a multi-modality imaging study and established a pioneering approach of synchronized computed tomography (CT) and digital radiography (DR), based on X-ray projections derived from three-dimensional CT reconstructed images. Whereas this approach can be applied in any clinical field, as a case of outstanding importance and great concern for zoological institutions, we selected foot bone pathologies in captive rhinoceroses to demonstrate the manifold applications of the method. Several advances were achieved, endowing the wildlife clinician with all-important tools: prototype DR exposure protocols and a modus operandi for foot positioning, advancing both traditional projections and, for the first-time, species-related radiographic views; assessment of radiographic diagnostic value for the whole foot and, in premiere, for each autopodial bone; together with additional insights into radiographic appearance of bone anatomy and pathology with a unique, simultaneous CT-DR correlation. Based on its main advantages in availing a wide range of keystone data in wildlife imaging from a limited number of examined subjects and combining advantages of CT as the golden standard method for bone diseases' diagnostic with DR's clinical feasibility under field conditions, synchronized CT-DR presents a new perspective on wildlife's health management. With this we hope to provide veterinary clinicians with concrete imaging techniques and substantial diagnostic tools, which facilitate straightforward attainment and interpretation of field radiography images taken worldwide.

  6. Rhinoceros Feet Step Out of a Rule-of-Thumb: A Wildlife Imaging Pioneering Approach of Synchronized Computed Tomography-Digital Radiography

    Science.gov (United States)

    Saragusty, Joseph; Göritz, Frank; Potier, Romain; Mulot, Baptiste; Maillot, Alexis; Etienne, Pascal; Bernardino, Rui; Fernandes, Teresa; Mews, Jurgen; Hildebrandt, Thomas Bernd

    2014-01-01

    Currently, radiography is the only imaging technique used to diagnose bone pathology in wild animals situated under “field conditions”. Nevertheless, while chronic foot disease in captive mega-herbivores is widely reported, foot radiographic imaging is confronted with scarcity of studies. Numerous hindrances lead to such limited numbers and it became very clear that the traditional perspective on bone imaging in domestic animals based on extensive studies and elaborated statistical evaluations cannot be extrapolated to their non-domestic relatives. For these reasons, the authors initiated a multi-modality imaging study and established a pioneering approach of synchronized computed tomography (CT) and digital radiography (DR), based on X-ray projections derived from three-dimensional CT reconstructed images. Whereas this approach can be applied in any clinical field, as a case of outstanding importance and great concern for zoological institutions, we selected foot bone pathologies in captive rhinoceroses to demonstrate the manifold applications of the method. Several advances were achieved, endowing the wildlife clinician with all-important tools: prototype DR exposure protocols and a modus operandi for foot positioning, advancing both traditional projections and, for the first-time, species-related radiographic views; assessment of radiographic diagnostic value for the whole foot and, in premiere, for each autopodial bone; together with additional insights into radiographic appearance of bone anatomy and pathology with a unique, simultaneous CT-DR correlation. Based on its main advantages in availing a wide range of keystone data in wildlife imaging from a limited number of examined subjects and combining advantages of CT as the golden standard method for bone diseases' diagnostic with DR's clinical feasibility under field conditions, synchronized CT-DR presents a new perspective on wildlife's health management. With this we hope to provide veterinary clinicians with concrete imaging techniques and substantial diagnostic tools, which facilitate straightforward attainment and interpretation of field radiography images taken worldwide. PMID:24963807

  7. The Kinetics of the Humoral and Interferon-Gamma Immune Responses to Experimental Mycobacterium bovis Infection in the White Rhinoceros (Ceratotherium simum)

    NARCIS (Netherlands)

    Parsons, Sven D.C.; Morar-Leather, Darshana; Buss, Peter; Hofmeyr, Jennifer; McFadyen, Ross; Rutten, Victor P M G; van Helden, Paul D.; Miller, Michele A.; Michel, Anita Luise

    2017-01-01

    Mycobacterium bovis is the cause of tuberculosis (TB) in a wide range of species, including white rhinoceroses (Ceratotherium simum). Control of the disease relies on the indirect detection of infection by measuring pathogen-specific responses of the host. These are poorly described in the white

  8. New species of Kiluluma Skrjabin, 1916 (Nematoda: Strongylida) from the white rhinoceros Ceratotherium simium (Burchell), with a redescription of K. solitaria Thapar, 1924.

    Science.gov (United States)

    Beveridge, Ian; Jabbar, Abdul

    2013-06-01

    Three species of Kiluluma Skrjabin, 1916 were identified in Ceratotherium simium (Burchell) from a captive population in New South Wales, Australia, based on analysis of the second internal transcribed spacer (ITS-2) of ribosomal DNA. One species was identified as K. solitaria Thapar, 1924 and is redescribed. A second species is new and is described here as K. ceratotherii n. sp. The third species is new but was represented by two individuals only and is described but is not named.

  9. Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros (Diceros bicornis)

    OpenAIRE

    Nijhof, Ard M.; Penzhorn, Banie L.; Lynen, Godelieve; Mollel, Johnson O.; Morkel, Pete; Bekker, Cornelis P. J.; Jongejan, Frans

    2003-01-01

    A novel Babesia species, designated Babesia bicornis sp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornis sp. nov.

  10. Post-release ranging behaviour of hand-raised black rhinoceros, Diceros bicornis, L. in Matusadona National Park, Zimbabwe with recommendations for management of introduction to the wild

    Directory of Open Access Journals (Sweden)

    G. Matipano

    2004-12-01

    Full Text Available Hand-raised rhinos would preferentially settle at sites close to areas where human activity was significant. Hand-raised rhinos that attached themselves to sites of human activity tended to move less widely and to have smaller seasonal ranges than those that moved away from human settlements. These rhinos had no fear of humans and some animals would move along established roads into hostile areas outside the park. These factors made hand-raised rhinos more vulnerable to poaching than their wild counterparts. Management of hand-raised rhinos with regards to ranging behaviour is discussed in the text.

  11. The Brain of the Black (Diceros bicornis and White (Ceratotherium simum African Rhinoceroses: Morphology and Volumetrics from Magnetic Resonance Imaging

    Directory of Open Access Journals (Sweden)

    Adhil Bhagwandin

    2017-08-01

    Full Text Available The morphology and volumetrics of the understudied brains of two iconic large terrestrial African mammals: the black (Diceros bicornis and white (Ceratotherium simum rhinoceroses are described. The black rhinoceros is typically solitary whereas the white rhinoceros is social, and both are members of the Perissodactyl order. Here, we provide descriptions of the surface of the brain of each rhinoceros. For both species, we use magnetic resonance images (MRI to develop a description of the internal anatomy of the rhinoceros brain and to calculate the volume of the amygdala, cerebellum, corpus callosum, hippocampus, and ventricular system as well as to determine the gyrencephalic index. The morphology of both black and white rhinoceros brains is very similar to each other, although certain minor differences, seemingly related to diet, were noted, and both brains evince the general anatomy of the mammalian brain. The rhinoceros brains display no obvious neuroanatomical specializations in comparison to other mammals previously studied. In addition, the volumetric analyses indicate that the size of the various regions of the rhinoceros brain measured, as well as the extent of gyrification, are what would be predicted for a mammal with their brain mass when compared allometrically to previously published data. We conclude that the brains of the black and white rhinoceros exhibit a typically mammalian organization at a superficial level, but histological studies may reveal specializations of interest in relation to rhinoceros behavior.

  12. The Brain of the Black (Diceros bicornis) and White (Ceratotherium simum) African Rhinoceroses: Morphology and Volumetrics from Magnetic Resonance Imaging.

    Science.gov (United States)

    Bhagwandin, Adhil; Haagensen, Mark; Manger, Paul R

    2017-01-01

    The morphology and volumetrics of the understudied brains of two iconic large terrestrial African mammals: the black ( Diceros bicornis ) and white ( Ceratotherium simum ) rhinoceroses are described. The black rhinoceros is typically solitary whereas the white rhinoceros is social, and both are members of the Perissodactyl order. Here, we provide descriptions of the surface of the brain of each rhinoceros. For both species, we use magnetic resonance images (MRI) to develop a description of the internal anatomy of the rhinoceros brain and to calculate the volume of the amygdala, cerebellum, corpus callosum, hippocampus, and ventricular system as well as to determine the gyrencephalic index. The morphology of both black and white rhinoceros brains is very similar to each other, although certain minor differences, seemingly related to diet, were noted, and both brains evince the general anatomy of the mammalian brain. The rhinoceros brains display no obvious neuroanatomical specializations in comparison to other mammals previously studied. In addition, the volumetric analyses indicate that the size of the various regions of the rhinoceros brain measured, as well as the extent of gyrification, are what would be predicted for a mammal with their brain mass when compared allometrically to previously published data. We conclude that the brains of the black and white rhinoceros exhibit a typically mammalian organization at a superficial level, but histological studies may reveal specializations of interest in relation to rhinoceros behavior.

  13. Histaminsko otrovanje morskom ribom

    Directory of Open Access Journals (Sweden)

    M. Nosić

    2010-01-01

    Full Text Available Histaminsko otrovanje je kemijska intoksikacija do koje dolazi kada se u organizam unese hrana (u ovom slučaju riba koja sadrži visoku koncentraciju histamina. Histamin se u ribi oblikuje post mortem, bakterijskom dekarboksilacijom esencijalne aminokiseline histidina. U ovom radu će se govoriti o uzrocima nastajanja histamina u ribi te o njegovu djelovanju na čovjekovo zdravlje te će ujedno biti navedeni načini prevencije ove vrste otrovanja. Ujedno će biti opisani slučajevi histaminskog otrovanja u našoj zemlji i u svijetu te će biti interpretirane stavke iz važećeg Pravilnika o mikrobiološkim kriterijima za hranu na temelju kojih se odlučuje o rezultatima kemijske analize na histamin.

  14. Professional strategies of medical officers of health in the post-war period--1: 'innovative traditionalism': the case of Dr Ian MacQueen, MOH for Aberdeen 1952-1974, a 'bull-dog' with the 'hide of a rhinoceros'.

    Science.gov (United States)

    Diack, Lesley; Smith, David F

    2002-06-01

    Recent policies concerning the enhancement of preventive medicine and health improvement have raised important questions about leadership in public health and have emphasized the roles that can be played by local authorities. In this light, it is worth exploring the activities undertaken by local authority Medical Officers of Health (MOsH), until their posts were abolished in 1974. The process leading to 1974 has often been blamed, at least partly, on the complacency, lack of imagination and demoralization of MOsH. However, when John Welshman asked the question 'watchdog or lapdog?' of the MOH, in a paper published in 1997, he concluded there was little justification for the latter label. This paper considers the career of Ian MacQueen, Aberdeen's last MOH, who is well known for the criticisms of his handling of the Aberdeen typhoid outbreak in 1964, which appeared in the report of an official enquiry. He was deemed to have made excessive use of the media and to have turned the outbreak into an event approaching a national crisis. However, in the context of MacQueen's 32 year career in Aberdeen, his use of the media during the typhoid outbreak was no aberration. Rather, it was characteristic of his determination to maintain an important role for the MOH within the NHS-era health services. There is therefore continuity between MacQueen's strategy and the ambitions of many MOsH before the NHS, who hoped for a unified health service with themselves occupying a leading role. MacQueen's actions during the typhoid outbreak also reflected his innovative activities in the field of health education, and his interest in the media for that purpose. In conclusion, MacQueen provides an example of an MOH who cannot be charged with complacency and resignation to a declining role: rather, his strategy of 'innovative traditionalism' sought to protect and extend his department's services.

  15. Development of single locus DNA microsatellite markers in Oryctes ...

    Indian Academy of Sciences (India)

    Oryctes rhinoceros, commonly known as rhinoceros bee- tle, is an important pest in oil palm plantations. The pres- ence of this pest in replanting sites as early as six months after replanting has alarmed planters due to the possi- bility of increased crop damage (Samsuddin et al. 1993;. Kamarudin and Wahid 1997). Being a ...

  16. The Visible Signature Modelling and Evaluation ToolBox

    Science.gov (United States)

    2008-12-01

    Rhinoceros . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 2.5.2 MODTRAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33...HYDROLIGHT. The commercial support software has a number of dif- ferent functionalities. Rhinoceros provides the wireframe models required as input...Greyscale texture synthesis takes a greyscale input image and a uniform white -noise texture. The white -noise texture is modified to reproduce certain

  17. 77 FR 21798 - Conference of the Parties to the Convention on International Trade in Endangered Species of Wild...

    Science.gov (United States)

    2012-04-11

    ... addition, we received comments from individuals as follows: 49 on the white rhinoceros; 25,742 on North.... Common snapping turtle (Chelydra serpentina)--Inclusion in Appendix III. Mammals 20. White rhinoceros...-bridged leaf turtle (Cyclemys pulchristriata), white-throated or southern snapping turtle (Elseya albagula...

  18. Vloga vohunov pri razvoju sovjetske atomske bombe : diplomsko delo visokošolskega strokovnega študija Varnost in policijsko delo

    OpenAIRE

    Križan, Petra

    2013-01-01

    Človek se je že od nekdaj zavedal, da je potrebno biti v koraku pred ostalimi, če želi biti uspešen in inovativen. Toda kako vedeti, da ni nasprotnik v razmišljanju, če ne celo v dejanjih že pred tabo? Za razblinjanje teh dvomov so tukaj nastopile osebe − vohuni, o katerih govori to diplomsko delo. Poskušala sem predstaviti pomembnost delovanja vohunov, še posebej pa sem se seveda osredotočila na njihov prispevek k razvoju sovjetske atomske bombe. Na samem začetku sem opisala pojme, ki so...

  19. DEFINICIJA I PODJELA ATIPIČNIH RANA

    OpenAIRE

    ŠITUM, MIRNA; KOLIĆ, MAJA

    2013-01-01

    Prema definiciji rana je prekid anatomskog i funkcionalnog kontinuiteta tkiva, a s obzirom na tijek cijeljenja rane mogu biti akutne i kronične. Kronične rane su one koje ne zarastaju unutar predviđenog razdoblja u korelaciji s etiologijom i lokalizacijom rane, a u najkraćem razdoblju od 6 tjedana. Kronične rane mogu biti tipične i atipične. u tipične rane ubrajaju se ishemijske rane, neuropatski ulkus, venski ulkus te dva posebna entiteta: dekubitus i dijabetičko stopalo. Kronične rane na po...

  20. Konceptualni pristup u uličnoj fotografiji

    OpenAIRE

    Toplek, Ana

    2017-01-01

    Tijekom godina pojavile su se mnoge teorije vezane uz uličnu fotografiju, s obzirom na niz aspekata, uključujući stil, pravila i ciljeve, kao i opću valjanost stila, jer ovu vrstu fotografije ne treba poduzeti na stvarnoj ulici, ona može biti snimljena i ako se uzme u zatvorenom prostoru, dok je to sve javno okruženje u kojem se snima. Ulična fotografija ima samo jedno strogo pravilo i to bi trebalo biti da pravi zrcalo društva, nepromijenjenu scenu sa nesvjesnim predmetim...

  1. PRIMERJALNA ANALIZA MOBILNIH REŠITEV CRM NA PRIMERU INTRIX CRM IN MICROSOFT DYNAMICS CRM

    OpenAIRE

    Tetičkovič, Rebeka

    2016-01-01

    Če želijo podjetja biti uspešna in biti konkurenca drugim podjetjem morajo uvesti v svoje podjetje rešitev za upravljanje odnosov s strankami, saj le tako lahko imajo celovit pogled na stranko in vidijo, kakšne so njihove potrebe in želje ter jih poskušajo zadvoljiti. Mobilne rešitve CRM podjetjem pomagajo, da lažje upravljajo odnose s strankami ne glede na to kje so. V uvodnem poglavju bomo opisali področje in opredelili problem, določili namen, cilje in hipoteze raziskave, določili predp...

  2. STILI VODENJA IN MOTIVACIJA V SLOVENSKIH ZAVAROVALNICAH

    OpenAIRE

    Petek, Alen

    2015-01-01

    Kako voditi oz. po kateri teoriji, stilu, avtorju so vprašanja katera se pojavljajo že dolgo časa. Ni pa dovolj, da smo vodje po teorijah, avtorjih, stilih, to so samo smernice, katere nam pomagajo, da lahko podrejene oz. sodelavce lažje pripeljemo do določenega cilja. Vodja mora biti v prvi vrsti oseba kateri zna poslušati, biti preudaren, marljiv delati včasih več kot podrejeni, da je plan izpolnjen. Razlika med vodji je različna, saj nekateri delujejo brez kakršne teoretične podlage drugi ...

  3. Haridustee = Education / Tiiu-Liisa Rummo-Laes

    Index Scriptorium Estoniae

    Rummo-Laes, Tiiu-Liisa

    2006-01-01

    Põhihariduses soolist eristumist ei ole märgata, murettekitavalt suur on poiste väljalangemine enne põhihariduse omandamist. Tütarlapsed ja noormehed üld- ja kutsehariduses, kesk- ja kõrghariduses. Noorte õppimine ja töötamine leibkonnatüübiti. Tabel, graafik, diagrammid

  4. Modern weapons and military equipments for issue 3-2017 / Современное вооружение и военное оборудование, к выпуску № 3-2017 / Savremeno naoružanje i vojna oprema za broj 3-2017

    Directory of Open Access Journals (Sweden)

    Dragan M. Vučković

    2017-07-01

    Full Text Available Wireless Technology Brings Crosshair from Thermal Sights to Night Vision Goggle Display; More bang for Bundeswer behemonts; The F-35 Stealth Fighter May Never Be Ready for Combat. / Nišanjenje putem bežične tehnologije; Novi opasniji leopard; F-35 možda nikada neće biti spreman za borbu.

  5. Effects of Puff-Adder Venom on Coagulation, Fibrinolysis and ...

    African Journals Online (AJOL)

    The in vitro and in vivo haematological effects of puffadder (Bitis arietans) venom in the baboon (Papio ursinus) with regard to its effect on coagulation, fibrinolysis and platelet aggregation were studied. There is a delay in the intrinsic coagulation mechanism with fibrinolysis and in vitro fibrinogenolysis. Normal human ...

  6. Characterization of a hemorrhage-inducing component present in ...

    African Journals Online (AJOL)

    Better characterization of individual snake venom toxins is useful for analyzing the association of their toxic domains and relevant antigenic epitopes. Here we analyzed the Bitis arietans hemorrhagic-inducing toxin present in a representative venom sample. Among the 1´ to 5´ protein peaks isolated using a Sephacryl S 100 ...

  7. Classifying snakebite in South Africa: Validating a scoring system

    African Journals Online (AJOL)

    There are some 38 venomous snake species in South Africa (SA), of which approximately half are dangerous to humans.[1] Snake species and density vary across the regions of SA.[2] Prevalent species include the Mozambique spitting cobra (Naja mossambica) and puff adder. (Bitis arietans), an elapid and viperid ...

  8. South African red data book - large mammals

    CSIR Research Space (South Africa)

    Skinner, JD

    1977-11-01

    Full Text Available Data sheets are provided for 22 threatened South African large mammals, one exterminated (Liechtenstein1s hartebeest), eight endangered (cheetah, hunting dog, dugong, Cape mountain zebra, black rhinoceros, tsessebe, roan antelope, suni), one...

  9. Mapping community vulnerability to poaching: A whole-of-society approach

    CSIR Research Space (South Africa)

    Schmitz, Peter MU

    2017-01-01

    Full Text Available Wildlife crime, especially rhinoceros poaching is very predominant in South Africa. Various approaches ranging from very sophisticated near-military interventions to engaging with communities are currently employed to reduce the levels of wildlife...

  10. The nutritional value of fourteen species of edible insects in ...

    African Journals Online (AJOL)

    hope&shola

    2006-02-02

    Feb 2, 2006 ... Pyrgomorphidae. Zonocerus variegatus. Grasshopper. Tata adults. Orthoptera. Acrididae. Cytacanthacris naeruginosus unicolor. Short horned grasshoppers. Tata adult. Coleoptera. Scarabaeidae. Analeptes trifasciata. Rhinoceros beetle. Ipe larvae. Coleoptera. Scarabaeidae. Oryctes boas. Scarab beetles.

  11. POPs and stable isotopes in bird and forage fish tissues - Persistent organic pollutant levels in juvenile salmonids, forage fish and their avian predators from Puget Sound and the outer WA coast

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is examining contaminant loads of fish prey species of a resident marine bird (Rhinoceros Auklet) breeding in inland waters (Puget Sound) and in the...

  12. Concept Design Report for a Low draft Stabilized - High Speed Connector (LDS-HSC) Vessel for the ONR High Speed Sea Lift (HSSL) Program

    Science.gov (United States)

    2007-04-30

    utilizing the Non-Uniform Rational B-Splines (NURBS) modeling software Rhinoceros . The detailed geometry is required to support the panelization and...revised baseline HSSL design were again generated offline utilizing Rhinoceros , as shown in Figure 2-10. The overall geometry concept is generally...chemical plants. Bill White at NAVSEA had tracked them down when challenged by those who said that no one had ever built large fans that could lift

  13. Defense Science Board 2006 Summer Study on 21st Century Strategic Technology Vectors. Volume 4. Accelerating the Transition of Technologies into U.S. Capabilities

    Science.gov (United States)

    2007-04-01

    a 50 percent reduction in cycle time;” a 1996 White House report requested a “25 percent cycle time reduction for major defense acquisition...War II example in the sidebar below. 30 I CH APT ER 2 The Rhinoceros : A World War II Innovation A well-known example of American...wartime innovation is the development and application of the “ Rhinoceros ” or “Rhino” hedgerow buster. This was typical of the process of decentralized

  14. Hydrostatic and Hydrodynamic Analysis of a Lengthened DDG-51 Destroyer Modified Repeat

    Science.gov (United States)

    2010-06-01

    Microsoft Excel, Rhinoceros , and a variety of ship resistance estimation codes to verify that the concept will work and attain some of the basic...to the size of the DDG-1000 at 14,500 ltons. The displacement is determined by using a Computer Aided Design (CAD) program called Rhinoceros (Rhino...operating conditions (speed, wave direction, and sea state) that the ship can safely operate and conduct its mission, where the white represents when

  15. Single High Fidelity Geometric Data Sets for LCM - Model Requirements

    Science.gov (United States)

    2006-11-01

    triangles (.raw) to the native triangular facet file (.facet). The software vendors recommend the use of McNeil and Associates’ Rhinoceros 3D for all...surface modeling and export. Rhinoceros has the capability and precision to create highly detailed 3D surface geometry suitable for radar cross section... white before ending up at blue as the temperature increases [27]. IR radiation was discovered in 1800 but its application is still limited in

  16. JPRS Report, East Europe.

    Science.gov (United States)

    1990-10-03

    survive if it clarifies the fact that it is the party of the productive classes (engineers, white collar and blue collar workers, cooperative members...Destiny As in Ionesco’s " Rhinoceros ," in these relations neither I nor anyone else who feels himself to be an Albanian and cannot turn his back on...sent off to isolation. They certainly must feel like Ionesco’s Mr. Beranze in " Rhinoceros ," everyone around him is making the noises of a

  17. Mixing Effects of Pylon-Aided Fuel Injection Located Upstream of a Flameholding Cavity in Supersonic Flow

    Science.gov (United States)

    2005-03-01

    highlighted in white . Figure 9. Basic hardware configuration showing pylon and flameholding cavity Pressure readings were obtained from taps on...were modified to accept pylons 27 Several early designs were brainstormed and drawn using Rhinoceros ® software. One of the more popular...the pylon attachments led to that idea being rejected. Rhinoceros ® renderings of this concept are shown in Figure 17. Figure 17. Early concept

  18. Diversity of methanogens in the hindgut of captive white rhinoceroses, Ceratotherium simum

    OpenAIRE

    Luo, Yu-heng; Wright, Andr?-Denis G; Li, You-long; Li, Hua; Yang, Qi-hong; Luo, Ling-juan; Yang, Ming-xian

    2013-01-01

    Background The white rhinoceros is on the verge of extinction with less than 20,200 animals remaining in the wild. In order to better protect these endangered animals, it is necessary to better understand their digestive physiology and nutritional requirements. The gut microbiota is nutritionally important for herbivorous animals. However, little is known about the microbial diversity in the gastrointestinal tract (GIT) of the white rhinoceros. Methanogen diversity in the GIT may be host spec...

  19. Biomarkers of Exposure to Toxic Substances. Volume 3: Proteomics, Biomarkers to Kidney and Organ Damage

    Science.gov (United States)

    2009-05-01

    precursor - Ceratotherium simum ( White rhinoceros ) (Square-lipped rhinoceros ) 0.551 0.699 Heterogeneous nuclear ribonucleoprotein K - Rattus...probed with two different antibodies: GSC 1239 and GSC 1242. Antibody GSC 1239 exhibited much higher background when it was used for the Western...blot analysis. Immunostainings confirmed the results of the Western blots. Only antibody GSC 1242 did not produce high nonspecific background . The

  20. Analiza panoge in SWOT analiza na primeru podjetja Golte

    OpenAIRE

    Kolar, Rebeka

    2012-01-01

    Za uspešnost podjetja je pomembno, da dobro poznamo svojo panogo, v kateri delujemo, dobro moramo poznati svojo konkurenco in biti vedno korak pred njo. Podrobno poznavanje panoge same pripomore k boljšemu in kvalitetnejšemu vrednotenju podjetja. SWOT analiza je pomembna za ugotavljanje konkurenčne prednosti podjetja in je ena najpogostejših in najpopularnejših analiz. Lahko jo izvajajo posamezniki ali skupina

  1. ANALIZA POSLOVNE KULTURE NA JAPONSKEM

    OpenAIRE

    Ocvirk, Katja

    2012-01-01

    Poslovanje na mednarodnih trgih se z globalizacijo vedno bolj spreminja. V delu diplomskega seminarja sem opredelila kulturo in opisala njene pomembne sestavine ter značilnosti. Spoznala sem, da mora biti poslovnež, ki deluje na mednarodnem trgu, zelo dobro pripravljen in seznanjen s kulturnimi razlikami, da se izogne nepotrebnim napakam in spodrsljajem. Menim, da moramo pri poslovanju s poslovnimi partnerji, ki prihajajo iz drugih kultur, vedeti, kako deluje njihovo poslovno okolje. Na ...

  2. Public health aspects of snakebite care in West Africa: perspectives from Nigeria

    OpenAIRE

    Habib, Abdulrazaq G

    2013-01-01

    Snakebite envenoming is a major public health problem among rural communities of the Nigerian savanna. The saw-scaled or carpet viper (Echis ocellatus) and, to a lesser extent, the African cobras (Naja spp.) and puff adders (Bitis arietans) have proved to be the most important cause of mortality and morbidity. The main clinical features of E. ocellatus envenoming are systemic hemorrhage, incoagulable blood, shock, local swelling, bleeding and, occasionally, necrosis. Bites may be complicated ...

  3. Les morsures de serpent en milieux pédiatriques à l'hôpital de l ...

    African Journals Online (AJOL)

    FAV-Afrique) actif sur les espèces Bitis, Echis, Naja et Dendroaspis de Sanofi Pasteur couplé au besoin d'un traitement symptomatique. Résultats: Un total de 56 dossiers de patients (sex-ratio 1,6; âge moyen = 9,36 ± 3,66 ans) ont été colligés ...

  4. Võidusammas muutus ohtlikuks ja isoleeriti aiaga / Urmas Jaagant

    Index Scriptorium Estoniae

    Jaagant, Urmas

    2010-01-01

    Vabaduse väljakul kukkus 5. juunil Vabadussõja võidusamba küljest alla klaasist nurgaliist. Rööbiti liistu allakukkumise põhjuse väljaselgitamisega kontrollitakse üle teised nurgaliistud. Kaitseministeerium on juhtunust teavitanud võidusamba ehitanud Tšehhi firmat Sans Souci. Liistujuhtum ei tohiks segada võidusamba uue valgustussüsteemi ehitamist

  5. Acute Effects of Anticholinesterase Agents on Pupillary Function.

    Science.gov (United States)

    1984-03-15

    evoked release is expressed as a re.ease ratio which ccrects for chances in spontaneous release. This release ratio is given by the formula EP-SR x...omesa- I-v1biti00 ’llow’fS t. re~o.e.n sele:tiiel. act’nates atelviciio- ration -we Lacteaed :i’s 4--solver. Tie p’eoaraiom was *nausled like

  6. Virusne bolesti krumpira

    OpenAIRE

    Vončina, Darko

    2013-01-01

    Krumpir može biti inficiran sa većim brojem virusa. U radu su prikazani simptomi, načini prijenosa te mjere kontrole za značajne viruse krumpira: virus uvijenosti lišća krumpira (PLRV), Y-virus krumpira (PVY), X-virus krumpira (PVX), A-virus krumpira (PVA), S-virus krumpira (PVS), virus mozaika lucerne (AMY) te virus žbunjavosti vrška krumpira (PMTV).

  7. Status Kritis Dua Jenis Badak di Indonesia

    Directory of Open Access Journals (Sweden)

    Haerudin R. Sadjudin

    2016-03-01

    Full Text Available There are five species of rhinoceros in the world, two types of African (Diceros bicornis and Ceratotherium simum and three species in Asia (Rhinoceros unicornis, Rhinoceros sondaicus and Dicerorhinus sumatrensis. Indonesia has two types, namely: the Javan rhino (Rhinoceros sondaicus Desmarest, 1822 and the Sumatran rhino (Dicerorhinus sumatrensis Fischer, 1814.Both types of rhino in Indonesia is not endemic wildlife, but it is a species relich; formerly spread is very wide, but now in addition to the steadily declining population is also more limited distribution. This happens because it is caused by the conversion of forests as a habitat for rhinos, plantations, settlements, and exploitation of space for various purposes. Hunting of two species of rhinoceros also has a long history, so that a distribution in some places have been lost; as well as the habitat that has been designated as a conservation area too, the population in the last 35 years continues to decline. Therefore both the rhino species has been categorized by the IUCN (International Union of Conservation of Nature and Natural Resources as critical or threatened species (Critical Endangered Species, highly endangered species. Due to the critical status, then efforts were made to save him. But it seems these efforts have not maximized, because not all potential or the ability  multi-party support to carry out rescue from extinction.

  8. Weyerhaeuser Export Facility at DuPont. Volume I.

    Science.gov (United States)

    1982-05-01

    Melanitta erspicllata), Bonaparte’s gull (Larus---hladelphia), common murre (Urla aalge), pigon guillemot (Cepphus Col-ma), and rhinoceros auklet (Ceror...Common murres, pigeon guillemots, glaucous winged (Larus glauscescens) and Bonaparte’s gulls and lesser numbers of rhinoceros aukle ae also present on...Gavia immer), western grebes, white -winged scoters (elanitta deglandi), and gulls’are regularly 63 g j~~~~~~~n Ve motatWtrbr GrE28 m~~~TVr Sc n Mie ra

  9. A Two-Phase Spherical Electric Machine for Generating Rotating Uniform Magnetic Fields

    Science.gov (United States)

    2007-06-01

    Fields by the Superposition of Current Loops B.2 RHINOCEROS ® 3.0 CAD/CAM B.3 OMAX® Layout and Make C Fluxball Construction C.1 Fabrication of Parts C.2...Chapter 6. Because of these complexities, a computer model was generated in Matlab and rendered in Rhinoceros 3.0 [41,42]. These tools allowed for rapid...verify the current carrying capacity of the 20 AWG wire bundle. Two 1 cm high bundles of 56 turns were wrapped in series on a 7.5 cm diameter white

  10. 78 FR 9725 - Endangered Species; Receipt of Applications for Permit

    Science.gov (United States)

    2013-02-11

    .... cottoni), Sumatran rhinoceros (Dicerorhinus sumatrensis), cheetah (Acinonyx jubatus), Pakistan sand cat... six captive-born tigers (Panthera tigris) and an additional nine tigers and one Bengal tiger (P.t...; 058668, Vijay; 058736, Ravi; Bengal tiger--182594, Sissy; and 058667, Nakita. This notification covers...

  11. COMPARATIVE ASPECTS OF PLANT-CELL WALL DIGESTION IN INSECTS

    NARCIS (Netherlands)

    PRINS, RA; KREULEN, DA

    Although many phytophagous and wood-eating invertibrates form their own cellulases, there is an overwhelming variety of symbioses between plant- and wood-utilising insects and microorganisms. In one type of symbiosis (endosymbiosis), insects (rhinoceros beetle, cockroach, lower termites) host

  12. Geometry and evolutionary parallelism in the long bones of cavioid ...

    Indian Academy of Sciences (India)

    Alexander R McN, Jayes AS, Maloiy GMO and Wathuta EM 1979. Mechanical stresses in fast locomotion of buffalo (Syncerus caffer) and elephant (Loxodonta africana). J. Zool. London 189 135–144. Alexander R McN and Pond CM 1992 Locomotion and bone strength in the white rhinoceros, Ceratotherium simum. J. Zool.

  13. Keeping the horn on the rhino: a Study of Balule Nature Reserve

    NARCIS (Netherlands)

    E Reuter (Elisa); L.C.J. Bisschop (Lieselot)

    2016-01-01

    textabstractSince 2007, rhinoceros poaching in South Africa has been increasing, now averaging three rhinos per day. Protecting the remaining rhinos requires both a better understanding of the crime of rhino poaching and of anti-poaching strategies. Through a theoretical triangulation of routine

  14. Notes on the biology of Oryctes monoceros (OLIV.) A pest of palms ...

    African Journals Online (AJOL)

    Studies on the biology of the rhinoceros beetle, Oryctes monoceros Oliv. was conducted in the laboratory. O. monoceros has 3 larval instars. In the mean the egg stage lasted 11, the larval stages 75.6, the pupal stage 15.5 and the adults stage 51.6 days. The mean developmental period from egg to adult for O. monoceros ...

  15. The mammals of Kilimanjaro | Grimshaw | Journal of East African ...

    African Journals Online (AJOL)

    One species, the black rhinoceros, is reported to have become extinct; another, the klipspringer, does not now occur in the area. Evidence for its alleged former presence and extinction is assessed. Red deer, introduced to Kilimanjaro during the German Colonial period, is now extinct on the Mountain. Other reported ...

  16. Contrasted patterns of evolution of the LINE-1 retrotransposon in perissodactyls: the history of a LINE-1 extinction.

    Science.gov (United States)

    Sookdeo, Akash; Hepp, Crystal M; Boissinot, Stéphane

    2018-01-01

    LINE-1 (L1) is the dominant autonomously replicating non-LTR retrotransposon in mammals. Although our knowledge of L1 evolution across the tree of life has considerably improved in recent years, what we know of L1 evolution in mammals is biased and comes mostly from studies in primates (mostly human) and rodents (mostly mouse). It is unclear if patterns of evolution that are shared between those two groups apply to other mammalian orders. Here we performed a detailed study on the evolution of L1 in perissodactyls by making use of the complete genome of the domestic horse and of the white rhinoceros. This mammalian order offers an excellent model to study the extinction of L1 since the rhinoceros is one of the few mammalian species to have lost active L1. We found that multiple L1 lineages, carrying different 5'UTRs, have been simultaneously active during the evolution of perissodactyls. We also found that L1 has continuously amplified and diversified in horse. In rhinoceros, L1 was very prolific early on. Two successful families were simultaneously active until ~20my ago but became extinct suddenly at exactly the same time. The general pattern of L1 evolution in perissodactyls is very similar to what was previously described in mouse and human, suggesting some commonalities in the way mammalian genomes interact with L1. We confirmed the extinction of L1 in rhinoceros and we discuss several possible mechanisms.

  17. EFFECT OF INFESTATION OF Dermestes maculatus on the ...

    African Journals Online (AJOL)

    Olusola Fasunwon

    2011-12-07

    Dec 7, 2011 ... Grasshoppers, Rhinoceros beetles, Caterpillars, Termites, Bees, Wasps, and Broods. (larvae and ... in the tropics [3]. The problems with utilizing insects' proteins are the lack of social acceptance, ... development is prolonged and larval mortality increases with increase in salt content. In experiments at 30. 0.

  18. Dietary overlap between Boer goats and indigenous browsers in a ...

    African Journals Online (AJOL)

    The winter diet of free ranging Boer goats in Valley Bushveld, KwaZulu-Natal, was determined by direct observations and compared with the diet of indigenous browsers (kudu, eland, giraffe, black rhinoceros) in order to determine which browsers are most compatible with goats for ensuring more efficient use of savanna ...

  19. Browse Title Index

    African Journals Online (AJOL)

    Items 201 - 210 of 210 ... Vol 15 (1991), Trichinella Spiralis in a White Rhinoceros, Abstract. O Bwangamoi, J Richardson, P D Sayer. Vol 31, No 2 (2007), Trypanotolerance effect as a result of genomic imprinting in F2 murine population, Abstract. JM Kamau, OA Mwai, PW Kinyanjui, FA Iraqi. Vol 30, No 1 (2006), Use of a ...

  20. Journal of Genetics | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    pp e92-e96. Development of single locus DNA microsatellite markers in Oryctes rhinoceros (Linnaeus) using 5′ anchored RAMs-PCR method · G. Manjeri R. Muhamad Q. Z. Faridah S. G. Tan · More Details Fulltext PDF. pp e97-e99. Novel polymorphic microsatellites from Florida red tilapia and cross-species amplification ...

  1. Design of parametric software tools

    DEFF Research Database (Denmark)

    Sabra, Jakob Borrits; Mullins, Michael

    2011-01-01

    fulfilment of evidence-based design criterion regarding light distribution and location in relation to patient safety in architectural health care design proposals. The study uses 2D/3D CAD modelling software Rhinoceros 3D with plug-in Grasshopper to create parametric tool prototypes to exemplify...

  2. Building energy demand aggregation and simulation tools

    DEFF Research Database (Denmark)

    Gianniou, Panagiota; Heller, Alfred; Rode, Carsten

    2015-01-01

    , which is based on monthly quasi-steady state calculations, using a visual parametric programming language (Grasshopper) coupled with a 3D design interface (Rhinoceros). The estimated heat demand of the examined houses from both simulation tools is compared to actual measured data of heat consumption...

  3. Research Article

    African Journals Online (AJOL)

    2016-09-01

    Sep 1, 2016 ... for Rhinoceros/Grasshopper software is used as the main tool, given its ability to effectively calculate daylight ... solver Octopus is adopted in order to achieve close to optimum results by controlling iteration .... Useful Daylight Illuminance represents the annual illumination distribution for a space to reach a ...

  4. Dermatologia comparativa: fibroqueratoma digital adquirido Comparative dermatology: acquired digital fibrokeratoma

    Directory of Open Access Journals (Sweden)

    Roberto Rheingantz da Cunha Filho

    2008-04-01

    Full Text Available Demonstra-se quadro característico de fibroqueratoma digital adquirido em trabalhadora rural de 42 anos de idade, que se compara a corno de rinoceronte.It is presented a case of a 42 year-old white female farmer with the classical feature of acquired digital fibrokeratoma, which is compared to rhinoceros horn.

  5. The effect of grassland shifts on the avifauna of a South African ...

    African Journals Online (AJOL)

    Two distinct grassland types occur within Hluhluwe-iMfolozi Park (HiP): short stoloniferous grazing lawns and tall, tussocklike 'bunch' grasslands. Grazing lawns are maintained by grazing mammals, among which White Rhinoceros Ceratotherium simum is of major importance. By contrast, tall bunch grasslands are ...

  6. Author Details

    African Journals Online (AJOL)

    Owen-Smith, Norman. Vol 7, No 1 (1972) - Articles Territoriality: the example of the White Rhinoceros Abstract PDF · Vol 31, No 3 (1996) - Articles Abundance and guild structure of grasshoppers (Orthoptera: Acridoidea) in communally grazed and protected savanna. Abstract PDF. ISSN: 2224-073X. AJOL African Journals ...

  7. Ground-Based Midcourse Defense (GMD) Initial Defensive Operations Capability (IDOC) at Vandenberg Air Force Base Environmental Assessment

    Science.gov (United States)

    2003-08-28

    deer. Birds such as ring-billed, Heerman’s, and glaucous-winged gulls, as well as the western wood-pewee, rhinoceros auklet, red-winged blackbird...of North Alabama Years of Experience: 10 Rebecca J. White , Environmental Specialist, EDAW, Inc. B.S., 2000, Civil/Environmental Engineer

  8. Author Details

    African Journals Online (AJOL)

    Bwangamoi, O. Vol 15 (1991) - Articles Trichinella Spiralis in a White Rhinoceros Abstract · Vol 15 (1991) - Articles Rabies, Microbesnoitosis and Sarcosystosis in a Lioness From Nairobi National Park Abstract · Vol 18 (1994) - Articles Norcardiosis in Dogs in Zimbabwe: Two Cases Abstract · Vol 18 (1994) - Articles

  9. DOD Is Not IBM: The Challenges of Implementing Strategic Sourcing in Defense Acquisition

    Science.gov (United States)

    2006-04-30

    most out of every dollar spent. “For nearly four decades, Congress, the media and the White House have figuratively and literally hammered the...mission. Industry has shown us that strategic sourcing is a powerful tool for reducing cost. But just as a hammer is of little use to a rhinoceros

  10. Author Details

    African Journals Online (AJOL)

    van der Goot, A.C.. Vol 48, No 1 (2013) - Articles Faecal progestagen profiles in wild southern white rhinoceros (Ceratotherium simum simum) Abstract. ISSN: 2224-073X. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms ...

  11. Cognitive and Instructional Theories of Impasses in Learning

    Science.gov (United States)

    1990-02-07

    Rhinoceros , Kangaroo, Elephant), Orientation (four primary compass values), and Noise Type (straight or curved). Combinations of these variables produced 40...Consultant NYNEX Cognitive & Instructional Sciences 500 Westchester Avenue 2520 North Vernon Street White Plains, NY 10604 Arlington, VA 22207 H. William

  12. Black Rhino Translocations within Africa | Knight | Africa Insight

    African Journals Online (AJOL)

    The responsibility for the conservation of the critically endangered Black Rhinoceros, Diceros bicornis, lies within Africa. This species is managed at the subspecies level and we document nine international translocation case studies involving South Africa since 1962 aimed at re-establishing or boosting populations ...

  13. Untitled

    Indian Academy of Sciences (India)

    In Coleoptera, generally the ninth sternite is either absent or present in the form of an X-shaped structure, the spiculum gastrale. In Oryctes rhinoceros the ninth sternite is represented by the spiculum gastrale. The phallus is an elongate, highly chitiinised structure measuring about. 15mm. in length. It is divisible into two parts ...

  14. Horn's Biologically Active Substances - Can We Replace Horns of Critically Endangered Species (Saiga) by Horns of More Abundant Animals?

    Czech Academy of Sciences Publication Activity Database

    Mikšík, Ivan; Romanov, O.

    2017-01-01

    Roč. 7, č. 1 (2017), s. 3-11 ISSN 2210-3155 R&D Projects: GA ČR(CZ) GA15-01948S Institutional support: RVO:67985823 Keywords : biologically active compounds * horn * rhinoceros * saiga * traditional Chinese medicine Subject RIV: CB - Analytical Chemistry, Separation OBOR OECD: Analytical chemistry

  15. The feeding behaviour of the redbilled oxpecker

    African Journals Online (AJOL)

    1981-04-22

    Apr 22, 1981 ... was found in the utilization of the white and black rhino- ceros. They have a naked substrate, few tick species that are utilized, large and open nostrils and are tolerant. Wound feeding was not taken into consideration because of the absence of filariid infested skin lesions in white rhinoceros (Schultz & Kluge ...

  16. Simptomatologija štetnih učinaka pesticida - pregled literature

    OpenAIRE

    Titlić, Marina; Punda, Ante; Jukić, Ivana; Tonkić, Ante; Josipović-Jelić, Željka

    2007-01-01

    Poljoprivrednici koji su kronično izloženi niskim razinama pesticida rijetko pokazuju klinički značajne znakove i simptome. Trovanje pesticidima može biti akutno i kronično. Primarna ciljna tkiva toksičnosti su krvotvorni sustav, kardiovaskularni sustav, reprodukcijski sustav i živčani sustav. Kod izloženih osoba pesticidi mogu uzrokovati mutacije gena i poremećaje kromosoma. Blaži, prolazni simptomi trovanja su često glavobolja, mučnina, povraćanje, omaglica, proljev, bolovi u trbuhu, bolovi...

  17. Are U.S. Naval Hospitals Operated Efficiently: A Study Using Diagnosis Related Groups

    Science.gov (United States)

    1986-12-01

    and Poorly Differentiated 10. nfectious and Parastio Ifectlousa md Parasitic 2,374 9 Diseases (Systemic or Diseases ULnpdifod Sites) 19. Mental Diseases...underscores this point in a message he makes to physicians: How to play games with the DRG payment system may become a popular parlor pastime, and...DISOIDERS AGE (70 Io C. C. .6975 1.7 26 M25 001 M NERVOUS SYSTEM INFECTION EXCEPT VIRAL MENINiBITIS 1.3141 7.6 28 021 001 M VIRAL MENINGITIS .6301 4.5

  18. Prirodni lijekovi u terapiji mentalnih poremećaja

    OpenAIRE

    Jakovljević, Miro; Mihaljević-Peleš, Alma; Šagud, Marina; Rajačić, Miro

    2002-01-01

    Sve je veći interes i u Hrvatskoj za liječenje prirodnim metodama uključujući i biljne lijekove. Liječenje kako somatskih tako i mentalnih poremećaja treba biti utemeljeno na znanstvenim činjenicama (tzv. evidence based medicine). U ovom članku daje se prikaz najvažnijih prirodnih lijekova koji mogu poslužiti u liječenju mentalnih poremećaja, a uključuje njihove kliničke učinke, mehanizme djelovanje, interakcije s drugim lijekovima i nuspojave. Informacije su rezultat ...

  19. Učinkovitost panela pri senzorskoj procjeni meda

    OpenAIRE

    Šurić, Pina

    2016-01-01

    Senzorska analiza je znanstvena disciplina koja potiče, mjeri, analizira i interpretira reakcije onih karakteristika hrane i tvari koje se zapažaju osjetilima vida, mirisa, okusa, dodira i sluha. Senzorski panel provodi senzorsku analizu. Senzorski ocjenjivači moraju biti školovani i motivirani da bi se dobila kvalitetna procjena. Program Panelcheck služi za brzu analizu jednog ili više panela. Program omogućava jednostavni grafički prikaz rezultat koji sadrže statističke podatke, a vizualizi...

  20. ZAŠČITA IN VARNOST INFORMACIJSKEGA SISTEMA V HOTELU

    OpenAIRE

    Mavrin, Marko

    2016-01-01

    Razvoj informacijsko-komunikacijskih tehnologij (IKT) skozi zadnja leta v hotelskih podjetjih kaže na številne pridobitve in priložnosti za izboljšanje poslovanja ter pridobivanja konkurenčne prednosti. Vsaka noviteta s seboj prinese tudi nova varnostna tveganja in nevarnosti, na katere mora biti podjetje pripravljeno. Ker so za uspešno delovanje podjetja pomembni podatki, predvsem pa obdelava podatkov, je ključno, da ne pride do razkritja podatkov tretji osebi. Varovanje in zaščita podatkov ...

  1. UTJELOVLJENJE I TEOLOGIJA RELIGIJA Kritički osvrt na neka novija tumačenja utjelovljenja

    OpenAIRE

    Bižaca, Nikola

    2008-01-01

    U aktualnim diskusijama teologije religija jedna od središnjih tema je shvaćanje utjelovljenja Božje Riječi. U uvodnim razmišljanjima autor posvješćuje važnost utjelovljenja unutar jedne teologije religija koja želi biti kršćanska te stoga uvažava objavljenu kristocentričnost Božjeg stvaralačko-spasenjskog projekta, ali i dijaloški realistična te je otvorena za Božje djelovanje na prostorima drugih religijskih tradicija. Slijedi potom prikaz nekih vidova poimanja utjelovl...

  2. Problemi sodobnega v starševstva kot izziv za v nadaljnje izobraževanje. Rezultati raziskave

    Directory of Open Access Journals (Sweden)

    Nives Ličen

    2004-12-01

    Full Text Available Avtorice prikazujejo rezultate raziskave, ki sodi na podrocje druzinske andragogike. Ugotavljajo, da sodobno izobrazevanje za druzinsko zivljenje izhaja iz potreb starsev, ki nimajo jasno izoblikovanih druzinskih vlog. DruŽinski clani se medsebojno vzgajajo, ko konstruirajo svojo biografijo. Starsi so zaskrbljeni za svoje otroke, zelijo jim čimvec ponuditi, da bi hili v zivljenju uspdni, hkrati od otrok tudi veliko pricakujejo. Potrebujejo znanja o druZinskem zivljenju in znanja, kako se ob otrocih uCiti. Izobrazevanje mora biti zaradi hitrih sprememb in individualiziranih druzinskih zgodb inovativno.

  3. Pentastomes (Pentastomida, Armillifer armillatus Wyman, 1848) in snakes from Zambia.

    Science.gov (United States)

    De Meneghi, D

    1999-12-01

    Twenty-three snakes, belonging to eight different species, were collected from rural areas of Zambia and inspected for the presence of pentastomes. Pentastomid parasites were found in four snakes: one African rock python (Python sebae), one puff adder (Bitis arietans) and two Mozambique spitting cobras (Naja mossambica) were infested with a small number of Armillifer armillatus, respectively five, two and one adult parasites. As humans can be incidental/intermediate hosts for reptilian pentastomes, the zoonotic potential of these parasites, especially in tropical countries, is discussed.

  4. A check-list of the nematode parasites of South African Serpentes (snakes) and Sauria (lizards).

    Science.gov (United States)

    Hering-Hagenbeck, S F; Boomker, J

    2000-03-01

    Published records, in combination with own data have been brought together to provide data on parasite/host relationships of reptiles that occur in the Republic of South Africa. A total of 62 nematode species belonging to 23 genera and 11 families are recorded from 20 snake and 21 lizard species. The genera Kalicephalus, Spauligodon, Ophidascaris and Abbreviata are especially well represented with between five and eight species per genus. The most nematode species were recorded from the flap-neck chameleon, Chamaeleo dilepis (eight), the puff-adder, Bitis arietans (eight) and the water monitor, Varanus niloticus (seven). All synonyms of parasites and hosts are given.

  5. PERCEPCIJA O HACCP NORMAMA I RAZINA KVALITETE IMPLEMENTACIJE HACCP-A U STRUCI

    OpenAIRE

    Bošnjak, Mirko

    2011-01-01

    U okviru obrazovnog programa VPŠ Utilus provode se obavezne studentske radionice istraživanja pojava i fenomena u struci, koje imaju za cilj istražiti i ukazati na trendove u stručnom okruženju, a koji su u direktnoj ili neposrednoj povezanosti s razinom kvalitete usluge. Prezentiranje rezultata istraživanja studentima podiže kvalitetu obrazovnog programa, a može biti od velike koristi nadležnim institucijama države u svrhu donošenja adekvatnih mjera i odluka. Is...

  6. Konzumerizam – društveni fenomen i nova ovisnost

    OpenAIRE

    Žakman-Ban, Vladimira; Špehar Fiškuš, Katarina

    2016-01-01

    U radu se raščlanjuje problem konzumerizma kao društvenoga fenomena, potrošački mentalitet kao suvremeni sustav vrijednosti i posljedično razvoj, karakteristike i mogući tretman poremećaja kupovanja kao nove ovisnosti. Fenomeni se obrađuju interdisciplinarno odnosno transdisciplinarno, a promatraju se kao „instant filozofija potrošačkog duha“. Ovisnici osjećaju pritisak biti „u trendu“ jer im agresivne korporativne marketinške kampanje nameću konzumerizam kao paradigmu uspješnoga života. Anks...

  7. Ranjivost obala otoka Raba zbog rasta razine mora

    OpenAIRE

    Ružić, Igor; Benac, Čedomir

    2016-01-01

    U ovom je radu opisana ranjivost obala otoka Raba koja može biti prouzročena prognoziranim porastom razine mora do kraja 21. stoljeća. Opisane su posljedice koju taj prirodni fenomen može prouzročiti. Analizirana su tri scenarija pojava visoke razine mora: kod stagnacije globalne morske razine te kod porasta za 30 i 60 cm. Analize su napravljene na topografskim kartama mjerila 1:5000. Ustanovljene su najugroženije urbanizirane obalne zone: uvala Sv. Eufemija, luka grada Raba, Supetarska Draga...

  8. Naša eko hrana

    OpenAIRE

    Kelnerić, Tatjana

    2013-01-01

    Zdrav način življenja trajna je zadaća održivog razvoja i može se reći da ne postoji dobna granica za početak takvog načina života. U tom kontekstu neizbježno je da cilj svakog odgojno-obrazovnog rada, bilo da je riječ o projektu ili svakodnevici jedne odgojno-obrazovne skupine u dječjem vrtiću, mora biti usmjeren na čisto i zdravo okruženje, neposredan kontakt djeteta s prirodom, pravilnu prehranu i kretanje.

  9. ZAKONODAVNI OKVIR UREĐENJA PROSTITUCIJE NA HRVATSKO-SLAVONSKOM PODRUČJU 1852. - 1929.

    OpenAIRE

    Pastović, Dunja; Željko, Darija

    2016-01-01

    U radu se prikazuje i analizira zakonodavni okvir uređenja prostitucije na hrvatsko-slavonskom području u razdoblju važenja Kaznenog zakona o zločinima,prijestupima i prekršajima iz 1852. Obilježje promatranog razdoblja je reglementaristički stav zakonodavca prema prostituciji koja se tolerirala kao “nužno zlo”, koje je moralo biti pod strogim nadzorom kako bi se suzbila potencijalna društvena šteta. Mjesnom redarstvu pripadao je nadzor i odlučivanje o tome hoće li prostituciju proganjati ili...

  10. Alati za poboljšavanje kvalitete

    OpenAIRE

    Čelar, Dražen; Valečić, Vladimir; Željezić, Dubravko; Kondić, Živko

    2014-01-01

    Kvaliteta je postala svjetski pokret i način življenja. Uspjeh organizacije najviše zavisi o kvaliteti proizvoda ili usluga. Za to najveću odgovornost snosi uprava, ali pri tome svi zaposleni moraju participirati u ostvarivanju kvalitete. Kvaliteta jest i biti će ključ za poboljšanje konkurentnosti na tržištu. Kvaliteta je značajan faktor opstanka, poslovnog uspjeha i prosperiteta poduzeća. Ključni princip sistema kvaliteta je donošenje odluka na osnovu činjenica, do kojih se dolazi prikuplja...

  11. Phytophthora fragariae Hickman i Phytophthora cactorum (Lebert & Cohn) J. Schröt na jagodi

    OpenAIRE

    Tomić, Željko

    2015-01-01

    Phytophthora fragariae i P. cactorum pripadaju među najdestruktivnije uzročnike bolesti u proizvodnji jagode. To su gljivicama slični organizmi koji napadaju podzemni dio biljke i uzrokuju venuće i sušenje jagode. Štete koje uzrokuju mogu biti drastične. Za razliku od P. fragariae, koja nikad nije zabilježena, P. cactorum često uzrokuje propadanje nasada jagode u Hrvatskoj. U radu su opisani etiologija i simptomi bolesti koje izazivaju te osnovna morfološka obilježja, naročito razlike među pa...

  12. Bolno rame

    OpenAIRE

    Mokrović, Hrvoje; Gulan, Gordan; Jotanović, Zdravko; Dragičević, Marin

    2009-01-01

    Bolno rame je jedno od najčešćih stanja s kojim se susreću liječnici u ortopediji i čini oko 20% patologije koštano-mišićnog sustava. Rameni je zglob zahvaljujući svojim biomehaničkim osobinama najpokretljiviji zglob u ljudskom tijelu, no ujedno i relativno nestabilan zglob, što ga čini posebno osjetljivim na ozljede. Uzroci bolnog ramena mogu biti različiti: upala i lezija tetiva rotatorne manžete, bolesti akromioklavikularnog zgloba, adhesivni kapsulitis, nestabilnosti ramena te artro...

  13. Rehabilitacija senzibiliteta. Filozofijska konstrukcija senzibilnog čovjeka

    OpenAIRE

    Zagorac, Ivana

    2010-01-01

    Osnovna nakana teksta je ukazati na neke bitne momente u filozofijskom tematiziranju senzibiliteta. Unutar tog pokušaja se izdvaja razdoblje prosvjetiteljstva koje se pak oslanja na specifično čitanje kartezijanske misli kroz empirizam engleske provenijencije. Jean-Jacques Rousseau je prepoznat kao ključna figura ‘doba senzibiliteta’ te će njegova konstrukcija senzibilnog čovjeka biti posebno detaljno razmotrena. Zaključno se naslijeđe povijesnih, a posebno istaknuto Rousseauovih, čitanja sen...

  14. Utjecaj ambalaže na području selektivne kozmetike i parfema

    OpenAIRE

    Ferenčak, Maja

    2015-01-01

    Završni rad bavi se izgledom ambalaže proizvoda selektivne kozmetike i skupocjenih parfema kroz identitet određenog branda, i njegovog krajnjeg korisnika-tržišta odnosno ciljane skupine. Dizajn ambalaže često ima presudan utjecaj na konačan izgleda proizvoda pogotovo u području o kojem pišem u završnom radu. Ambalaža selektivne kozmetike mora biti luksuzna i elegantna kako bi opravdala svoj naziv i pokazala ono što je izdvaja od takozvane „mass“ kozmetike. Ključna zadaća samog ...

  15. Problematika jamčevalnih zahtevkov potrošnikov z vidika "afere Volkswagen"

    OpenAIRE

    Peče, Žan

    2017-01-01

    V diplomski nalogi je obravnavana problematika jamčevalnih zahtevkov potrošnikov z vidika afere Volkswagen (Dieselgate), v kateri je bilo odkrito, da so v koncernu Volkswagen v svoja vozila nameščali skrito programsko opremo, ki je prirejala podatke o vrednosti izpušnih plinov v času testiranja vozil. V diplomski nalogi so predstavljeni jamčevalni zahtevki, katere imajo zaradi ugotovljene napake na vozilu na voljo potrošniki. Predstavljene so predpostavke, ki morajo biti izpolnjene, da ...

  16. MOŽNOST VGRADNJE PETERSENOVE DUŠILKE V OMREŽJE ELEKTRA CELJE D.D.

    OpenAIRE

    Savinek, Arnej

    2011-01-01

    V diplomskem delu so opisani različni načini ozemljitve nevtralne točke transformatorja. Podrobno je predstavljeno resonančno obratovanje preko Petersenove dušilke. Obdelani so pogoji, ki morajo biti izpolnjeni za zanesljivo in varno obratovanje transformatorja ozemljenega preko Petersenove dušilke. Določeni so kriteriji in izbrane razdelilne transformatorske postaje, ki so najbolj primerne za vgradnjo Petersenove dušilke v elektroenergetskem omrežju Elektra Celje d.d.. Predstavljeno ju tudi ...

  17. ANALIZA WELLNESS PONUDBE V IZBRANEM HOTELU NA PTUJU

    OpenAIRE

    Poljanec, Suzana

    2009-01-01

    Wellness je svetovno turistično gibanje sodobnega časa. Pomeni kakovost življenja in vključuje sestavine zdravega načina življenja. V celovito ponudbo wellnessa morajo biti vključene vse štiri temeljne sestavine - zdrava prehrana, osebna sprostitev, telesne aktivnosti in duševne aktivnosti. V diplomski nalogi sem raziskovala sedanje stanje wellness ponudbe v hotelu Mitra na Ptuju. Glavne ugotovitve raziskave so: • wellness postaja iz dneva v dan vse bolj pomemben segment turizma, sa...

  18. Nasilje u obitelji kao rizičan čimbenik razvoja djece

    OpenAIRE

    Ilić, Martina

    2015-01-01

    Konvencija UN-a o pravima djeteta nalaže da je pravo svakog djeteta da odrasta u obitelji u kojoj se osjeća sigurno, voljeno i zaštićeno. Obitelj bi za svako dijete trebala biti mjesto sreće, sigurnosti, međusobnog uvažavanja i poštivanja. Problem zlostavljanja i zanemarivanja djece postojao je u svim civilizacijama i kulturama, a kroz godine je postignut veliki pomak na tom području pa se danas sve više prepoznaju negativne i štetne posljedice koje nasilje u obitelji ostavlja na dijete i ...

  19. PRIMERJAVA DVEH METOD ZA MERJENJE ZADOVOLJSTVA GOSTOV V HOTELIH

    OpenAIRE

    Osterc, Miša

    2016-01-01

    Zadovoljstvo gostov je ključni element za uspešen razvoj in dolgoročen obstoj kateregakoli hotelskega podjetja, zato mora biti njihovo doseganje tudi glavni cilj vsakega hotela. Pri tem je potrebno upoštevati, da zagotavljanje zadovoljstva gostov zahteva dobro poznavanje želja in pričakovanj vsakega gosta, zato je merjenje, analiziranje in spremljanje zadovoljstva eden najpomembnejših izzivov pri pripravi strategije poslovanja vsakega hotela. To velja tudi za Hotel Bioterme Mala Nedelja,...

  20. Prednosti in slabosti preoblikovanja s.p. v d.o.o.

    OpenAIRE

    Tišma, Zoran

    2016-01-01

    Naloga lastnikov in vodilnih v podjetju je, da oblikuje bodočo predstavo o podjetju. Obstajati mora trajna želja po njegovi učinkovitosti, uspešnosti, poštenosti ter notranji in zunanji interes po trajnem obstoju in razvoju. Poslovanje je treba usmerjati, uravnavati in nadzirati, da lahko dosežemo zastavljene cilje, ki morajo biti skladni z vizijo in poslanstvom podjetja. Za uresničitev vizije, poslanstva in ciljev je treba sprejeti strateške odločitve, ki lahko pomembno vplivajo na poslo...

  1. ZASNOVA PORTALA ZA IZBOLJŠANJE SNOVANJA PISARNIŠKEGA OKOLJA PO NAČELIH ERGONOMIJE

    OpenAIRE

    Crnič, Damjan

    2016-01-01

    Dandanes vse več časa preživimo na delovnem mestu − pisarni. Prav zato je pomembno, da je okolje, v katerem opravljamo svoje delo prilagojeno našim potrebam. Pisarniško pohištvo in pripomočki morajo biti prilagojeni regulativnim zahtevam ter zahtevam delovnega procesa. Pri snovanju delovnega okolja je treba upoštevati načela ergonomije in za delo uporabljati ergonomsko oblikovano pohištvo in pripomočke, ki dokazano pripomorejo k večji učinkovitosti zaposlenih. V samem diplomskem delu smo...

  2. Zbrinjavanje medicinskog otpada - zakonodavstvo i njegova provedba

    OpenAIRE

    Marinković, Natalija; Vitale, Ksenija; Janev Holcer, Nataša; Džakula, Aleksandar

    2006-01-01

    Gospodarenje medicinskim otpadom, koji po svojim svojstvima može biti opasan i/ili inertan, u Hrvatskoj je regulirano trima temeljnim pravnim aktima: Zakonom o otpadu, Pravilnikom o vrstama otpada te Naputkom o postupanju s otpadom koji nastaje pri pružanju zdravstvene zaštite. Uz njih još su i Zakon o prijevozu opasnih tvari i Zakon o otrovima akti koji podupiru provedbu ovih propisa. Slijedom razvoja suvremenih cjelovitih sustava za gospodarenje otpadom razvijen je i cjeloviti sustav za gos...

  3. Microstructure-mediated Optical Effects in Southern African Snakes

    Directory of Open Access Journals (Sweden)

    Singh Ishan

    2017-01-01

    Full Text Available The scales of the African Viper Bitis arietans were tested for optical effects. Spectral intensity was recorded at incident angles over the visible spectrum for dark, pale, and ventral scale regions. The lowest spectral intensity recordings were associated with scales which have the greatest level of micro-structuring. Our results indicate that scale appearance in B. arietans is a product of microstructure-mediated optical effects. The optical effect may play a role in improving the ecological performance of the snake in its natural environment.

  4. Microstructure-mediated Optical Effects in Southern African Snakes

    Science.gov (United States)

    Singh, Ishan; Alexander, Graham

    2017-03-01

    The scales of the African Viper Bitis arietans were tested for optical effects. Spectral intensity was recorded at incident angles over the visible spectrum for dark, pale, and ventral scale regions. The lowest spectral intensity recordings were associated with scales which have the greatest level of micro-structuring. Our results indicate that scale appearance in B. arietans is a product of microstructure-mediated optical effects. The optical effect may play a role in improving the ecological performance of the snake in its natural environment.

  5. VARNOST ELEKTRONSKEGA POSLOVANJA

    OpenAIRE

    Marovt, Tjaša

    2016-01-01

    Elektronsko poslovanje je del našega vsakdanjega življenja in je skoraj že izpodrinilo klasično poslovanje. Vse večja uporaba elektronskega poslovanja prinaša vedno več groženj in nevarnosti. Sem spadajo nezaželena e-pošta, ribarjenje, računalniški virusi, računalniški črvi, zlonamerne programske opreme in ostale nevarnosti. Uporabniki morajo biti seznanjeni z nevarnostjo in se pred njimi zaščititi z ustreznimi varnostnimi mehanizmi, kot so šifriranje podatkov, elektronski podpis, digitalno p...

  6. One Small Step for Rhinos, One Giant Leap for Wildlife Management- Imaging Diagnosis of Bone Pathology in Distal Limb

    Science.gov (United States)

    Galateanu, Gabriela; Hildebrandt, Thomas B.; Maillot, Alexis; Etienne, Pascal; Potier, Romain; Mulot, Baptiste; Saragusty, Joseph; Hermes, Robert

    2013-01-01

    Chronic foot disease poses a threat to the general health, represents a tremendous clinical challenge, and often is a reason for euthanasia in captive megaherbivores, among them the elephant and rhinoceros. Nevertheless, apart from the elephant, foot pathology is handled as being confined only to soft tissues whereas bone pathology is often overlooked. As a case in point, the authors selected the second largest mammal on land, the rhinoceros. We performed a computed tomographic (CT) study using the highest resolution available in veterinary world, followed by digital radiography of eight distal limbs from two white and one Indian rhinoceroses. Our study demonstrated that bone pathology in rhinoceroses’ foot is present and in large numbers, yet none of these were diagnosed ante mortem. Even when the animals were euthanized due to foot problems, the decision was based on soft tissue pathology rather than orthopedic reasons. Even more worrying is the fact that the largest number of osteopathologies was present in one of the white rhinoceroses that showed no discernable related clinical signs. This study describes for the first time the existence of bone pathology in white rhinoceros foot, in addition to the two previously described rhinoceros species - Indian and black rhinoceroses. Furthermore, the chronic foot disease reported for the Indian rhinoceros in our study was not restricted to soft tissue structures as was presumed ante mortem but included severe bone pathology. New evidence suggesting that osteopathology in rhinoceroses’ distal limb is more widespread than it was thought before could force us to rethink of radiographic diagnosis in captive megaherbivores as routine examination incorporated into their health management. The anticipated improvements in radiologic examinations in megaherbivores will increase the effectiveness of their management and husbandry and open the way for improved animal welfare and better wildlife conservation. PMID:23874643

  7. Analysis of complete mitochondrial genomes from extinct and extant rhinoceroses reveals lack of phylogenetic resolution

    Science.gov (United States)

    Willerslev, Eske; Gilbert, M Thomas P; Binladen, Jonas; Ho, Simon YW; Campos, Paula F; Ratan, Aakrosh; Tomsho, Lynn P; da Fonseca, Rute R; Sher, Andrei; Kuznetsova, Tatanya V; Nowak-Kemp, Malgosia; Roth, Terri L; Miller, Webb; Schuster, Stephan C

    2009-01-01

    Background The scientific literature contains many examples where DNA sequence analyses have been used to provide definitive answers to phylogenetic problems that traditional (non-DNA based) approaches alone have failed to resolve. One notable example concerns the rhinoceroses, a group for which several contradictory phylogenies were proposed on the basis of morphology, then apparently resolved using mitochondrial DNA fragments. Results In this study we report the first complete mitochondrial genome sequences of the extinct ice-age woolly rhinoceros (Coelodonta antiquitatis), and the threatened Javan (Rhinoceros sondaicus), Sumatran (Dicerorhinus sumatrensis), and black (Diceros bicornis) rhinoceroses. In combination with the previously published mitochondrial genomes of the white (Ceratotherium simum) and Indian (Rhinoceros unicornis) rhinoceroses, this data set putatively enables reconstruction of the rhinoceros phylogeny. While the six species cluster into three strongly supported sister-pairings: (i) The black/white, (ii) the woolly/Sumatran, and (iii) the Javan/Indian, resolution of the higher-level relationships has no statistical support. The phylogenetic signal from individual genes is highly diffuse, with mixed topological support from different genes. Furthermore, the choice of outgroup (horse vs tapir) has considerable effect on reconstruction of the phylogeny. The lack of resolution is suggestive of a hard polytomy at the base of crown-group Rhinocerotidae, and this is supported by an investigation of the relative branch lengths. Conclusion Satisfactory resolution of the rhinoceros phylogeny may not be achievable without additional analyses of substantial amounts of nuclear DNA. This study provides a compelling demonstration that, in spite of substantial sequence length, there are significant limitations with single-locus phylogenetics. We expect further examples of this to appear as next-generation, large-scale sequencing of complete mitochondrial

  8. Analysis of complete mitochondrial genomes from extinct and extant rhinoceroses reveals lack of phylogenetic resolution

    Directory of Open Access Journals (Sweden)

    Nowak-Kemp Malgosia

    2009-05-01

    Full Text Available Abstract Background The scientific literature contains many examples where DNA sequence analyses have been used to provide definitive answers to phylogenetic problems that traditional (non-DNA based approaches alone have failed to resolve. One notable example concerns the rhinoceroses, a group for which several contradictory phylogenies were proposed on the basis of morphology, then apparently resolved using mitochondrial DNA fragments. Results In this study we report the first complete mitochondrial genome sequences of the extinct ice-age woolly rhinoceros (Coelodonta antiquitatis, and the threatened Javan (Rhinoceros sondaicus, Sumatran (Dicerorhinus sumatrensis, and black (Diceros bicornis rhinoceroses. In combination with the previously published mitochondrial genomes of the white (Ceratotherium simum and Indian (Rhinoceros unicornis rhinoceroses, this data set putatively enables reconstruction of the rhinoceros phylogeny. While the six species cluster into three strongly supported sister-pairings: (i The black/white, (ii the woolly/Sumatran, and (iii the Javan/Indian, resolution of the higher-level relationships has no statistical support. The phylogenetic signal from individual genes is highly diffuse, with mixed topological support from different genes. Furthermore, the choice of outgroup (horse vs tapir has considerable effect on reconstruction of the phylogeny. The lack of resolution is suggestive of a hard polytomy at the base of crown-group Rhinocerotidae, and this is supported by an investigation of the relative branch lengths. Conclusion Satisfactory resolution of the rhinoceros phylogeny may not be achievable without additional analyses of substantial amounts of nuclear DNA. This study provides a compelling demonstration that, in spite of substantial sequence length, there are significant limitations with single-locus phylogenetics. We expect further examples of this to appear as next-generation, large-scale sequencing of complete

  9. One small step for rhinos, one giant leap for wildlife management--imaging diagnosis of bone pathology in distal limb.

    Directory of Open Access Journals (Sweden)

    Gabriela Galateanu

    Full Text Available Chronic foot disease poses a threat to the general health, represents a tremendous clinical challenge, and often is a reason for euthanasia in captive megaherbivores, among them the elephant and rhinoceros. Nevertheless, apart from the elephant, foot pathology is handled as being confined only to soft tissues whereas bone pathology is often overlooked. As a case in point, the authors selected the second largest mammal on land, the rhinoceros. We performed a computed tomographic (CT study using the highest resolution available in veterinary world, followed by digital radiography of eight distal limbs from two white and one Indian rhinoceroses. Our study demonstrated that bone pathology in rhinoceroses' foot is present and in large numbers, yet none of these were diagnosed ante mortem. Even when the animals were euthanized due to foot problems, the decision was based on soft tissue pathology rather than orthopedic reasons. Even more worrying is the fact that the largest number of osteopathologies was present in one of the white rhinoceroses that showed no discernable related clinical signs. This study describes for the first time the existence of bone pathology in white rhinoceros foot, in addition to the two previously described rhinoceros species--Indian and black rhinoceroses. Furthermore, the chronic foot disease reported for the Indian rhinoceros in our study was not restricted to soft tissue structures as was presumed ante mortem but included severe bone pathology. New evidence suggesting that osteopathology in rhinoceroses' distal limb is more widespread than it was thought before could force us to rethink of radiographic diagnosis in captive megaherbivores as routine examination incorporated into their health management. The anticipated improvements in radiologic examinations in megaherbivores will increase the effectiveness of their management and husbandry and open the way for improved animal welfare and better wildlife conservation.

  10. Armillifer-Infected Snakes Sold at Congolese Bushmeat Markets Represent an Emerging Zoonotic Threat.

    Science.gov (United States)

    Hardi, Richard; Babocsay, Gergely; Tappe, Dennis; Sulyok, Mihály; Bodó, Imre; Rózsa, Lajos

    2017-12-01

    African pythons (Pythonidae) and large vipers (Bitis spp.) act as definitive hosts for Armillifer armillatus and Armillifer grandis parasites (Crustacea: Pentastomida) in the Congo Basin. Since the proportion of snakes in bushmeat gradually increases, human pentastomiasis is an emerging zoonotic disease. To substantiate the significance of this threat, we surveyed snakes offered for human consumption at bushmeat markets in the Kole district, Democratic Republic of the Congo, for the presence of adult pentastomids. In Bitis vipers (n = 40), Armillifer spp. infestations exhibited an 87.5% prevalence and 6.0 median intensity. Parasite abundance covaried positively with viper length, but not with body mass. In pythons (n = 13), Armillifer spp. exhibited a 92.3% prevalence and 3.5 median intensity. The positive correlations between parasite abundance and python length or mass were statistically nonsignificant. Ninety-one percent of A. grandis were discovered in vipers and 97% of infected vipers hosted A. grandis, whereas 81% of A. armillatus specimens were found in pythons and 63% of infected pythons hosted A. armillatus. Thus, challenging the widespread notion of strict host specificity, we found 'reversed' infections and even a case of coinfection. In this study, we also gathered information about the snake consumption habits of different tribal cultures in the area. Infective parasite ova likely transmit to humans directly by consumption of uncooked meat, or indirectly through contaminated hands, kitchen tools or washing water.

  11. Species-specific responses of Late Quaternary megafauna to climate and humans

    DEFF Research Database (Denmark)

    Lorenzen, Eline D; Nogués-Bravo, David; Orlando, Ludovic

    2011-01-01

    Despite decades of research, the roles of climate and humans in driving the dramatic extinctions of large-bodied mammals during the Late Quaternary period remain contentious. Here we use ancient DNA, species distribution models and the human fossil record to elucidate how climate and humans shaped...... the demographic history of woolly rhinoceros, woolly mammoth, wild horse, reindeer, bison and musk ox. We show that climate has been a major driver of population change over the past 50,000 years. However, each species responds differently to the effects of climatic shifts, habitat redistribution and human...... encroachment. Although climate change alone can explain the extinction of some species, such as Eurasian musk ox and woolly rhinoceros, a combination of climatic and anthropogenic effects appears to be responsible for the extinction of others, including Eurasian steppe bison and wild horse. We find no genetic...

  12. Protective Activity Against Oxidative Stress of Plants Indigenous to Korea

    International Nuclear Information System (INIS)

    Yoo, B.S.; Yang, Y.T.; Jung, M.S.; Kang, K.A.; Zhang, Rui; Chae, S.W.; Lee, N.H.; Park, J.W.; Hyun, J.W.

    2005-01-01

    We have screened the cytoprotective effect against Ha, Oa, and γ-ray radiation induced oxidative stress from 32 Korean plants. Betula ermani var, saitoana (caulis, leaves), Rosa wichuraiana (caulis), Sorbus commixta (caulis), Weigela florida (leaves), Cirsium rhinoceros (whole plant), and Viburnum erosum (caulis) were found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of six plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by Ha,Oa, treatment. In addition, these extracts protected cell death of V79-4 cells damaged by γ-ray radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Betula ermani var. saitoana, Rosa wichuraiana, Sorbus commixta, Weigela florida, Cirsium rhinoceros, and Viburnum erosum protect V79-4 cells against oxidative damage by radiation through scavenging ROS

  13. 1981 Image II Conference Proceedings.

    Science.gov (United States)

    1981-11-01

    INTRODUCTION The Computer Aided Design/Computer Aided Manufacturing (CAD/CAM) segment of the computer graphics industry has seen continuous growth during...matches to their experience base. An individual who sees a rhinoceros for the first time may perceive it as a horse with a horn and call it a unicorn ...ground growth as well as attitude (i.e., roll, pitch, yaw) information considered to be potentially useful as cues for improved aircraft control and visual

  14. A serological survey of brucellosis in wild ungulate species from five game parks in Zimbabwe

    Directory of Open Access Journals (Sweden)

    Tatenda R. Motsi

    2013-03-01

    Full Text Available A retrospective serosurvey was carried out between 2009 and 2012 to detect antibodies to Brucella spp. in free-ranging African wildlife ungulates from five selected game parks in Zimbabwe. Samples were drawn from wildlife-livestock interface and non-interface areas in Zimbabwe. A total of 270 serum samples from four different species, namely African buffalo (Syncerus caffer (n=106, impala (Aepyceros melampus (n = 72, black rhinoceros (Diceros bicornis (n= 45 and white rhinoceros (Ceratotherium simum (n = 47, were tested. The percentage of positive samples was 17.0% in buffalo (18/106; 95% CI: 9.72% – 24.1% and 1.4% in impala (1/72; 95% CI: 0% – 4.2%. No antibodies to Brucella spp. were detected in the two rhinoceros species. The difference in the percentage of seropositive cases between buffalo and impala was significant (p< 0.05. Seropositivity to Brucella spp. was higher (19.1% in adult buffalo compared with juveniles and sub-adults younger than six years (5.9%. Further, seropositivity was marginally higher (20.4% in animals from wildlife-livestock interface areas than in those from non-interface areas (13.45%; OR = 1.45 although the difference was not statistically significant. The study showed that brucellosis could be more widespread in buffalo and may circulate in this species independently in the absence of contact with cattle, whilst rhinoceros may be considered less susceptible to brucellosis. The role of the wildlife-livestock interface in the epidemiology of brucellosis in wildlife and livestock is probably overstated but needs to be explored further.

  15. Rewinding the process of mammalian extinction

    OpenAIRE

    Saragusty, J.; Diecke, S.; Drukker, M.; Durrant, B.; Friedrich Ben-Nun, I.; Galli, C.; Goeritz, F.; Hayashi, K.; Hermes, R.; Holtze, S.; Johnson, S.; Lazzari, G.; Loi, P.; Loring, J.F.; Okita, K.

    2016-01-01

    With only three living individuals left on this planet, the northern white rhinoceros (Ceratotherium simum cottoni) could be considered doomed for extinction. It might still be possible, however, to rescue the (sub)species by combining novel stem cell and assisted reproductive technologies. To discuss the various practical options available to us, we convened a multidisciplinary meeting under the name "Conservation by Cellular Technologies." The outcome of this meeting and the proposed road m...

  16. Timber Curtain: Designing with material capabilities

    DEFF Research Database (Denmark)

    Lahmy, Maya; Larsen, Niels Martin

    2015-01-01

    Timber Curtain explores relations between digital precision and material indeterminacy. It is an installation engaging spatially through its presence as a 1:1 architectural component as well as it is exploring novel technologies in the architectural design process from the very beginning of the g...... of pinewood, processed with a 5-axis CNC router. The digital process generation and simulation is implemented with Rhinoceros 3D, Grasshopper and GHPython, and AlphaCAM was used for preparing the CNC-milling....

  17. Propagation and Purification of Baculovirus oryctes Huger

    Directory of Open Access Journals (Sweden)

    Susamto Somowiyarjo

    1995-12-01

    Full Text Available An isolate of Baculovirus oryctes, a possible biological control agent for coconut beetle (Oryctes rhinoceros Huger from East Java was propagated and purified. The virus could be transmitted by feeding the imago with 10% sucrose containing virus from homogenate of infected beetles. Effectivity of virus to 9 healthy females by sexual copulation. Virus be succesfully purified by a method of Payne. Key words: Baculovirus oryctes, transmission, purification

  18. Isogeometric Collocation: Cost Comparison with Galerkin Methods and Extension to Adaptive Hierarchical NURBS Discretizations (Preprint)

    Science.gov (United States)

    2013-02-06

    the white collocation points have been truncated, although the basis functions remain 67 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Fine-scale B-splines: Coarse...geometric design. Morgan Kaufmann Publishers, 2002. [75] Rhinoceros — NURBS modeling for Windows. http://www.rhino3d.com/, 2012. [76] B.A. Finlayson

  19. Planning: Complex Endeavors

    Science.gov (United States)

    2007-04-01

    international crime (trafficking in ille- gal drugs, counterfeit consumer goods, banned substances such as ivory and rhinoceros horn, illegal immigration, and...Conflict_Capabilities_final.pdf 112Berger, Scowcroft, and Nash, In the Wake of War, 38. 113The White House, “The National Security Strategy Of the United States” (Washington...The White House, Sept 2002), 21. http://www.whitehouse.gov/nsc/nss.pdf 114Agency for International Development, “Foreign Aid in the National

  20. High-Performance Computing Opportunities and Challenges for Army R&D

    Science.gov (United States)

    2006-01-01

    UGS Unattended Ground Sensor WSMR White Sands Missile Range 1 CHAPTER ONE Introduction The Army’s Director for Research and Laboratory Management...networks. The White Sands Missile Range (WSMR) in New Mexico has a project called “Range Operations Real-Time Test Display System.” The project is...catalysts, inspired by blue mussel adhesive; and self-healing materials emulating a rhinoceros horn. We discuss two possible biomimicry applications

  1. Environmental Assessment: Base Realignment and Closure (BRAC) 2005 Mission Realignments to Vandenberg AFB

    Science.gov (United States)

    2007-02-01

    birds such as Bewick’s wren, California quail, Spotted and California towhees, White -crowned and Song sparrows, and Anna’s hummingbird; reptiles...Additional Comments White -faced ibis (rookery site) Plegadis chihi CSC O Migrant Freshwater marshes, ponds Flock observed at Barka Slough...round Beaches and coastal dunes Marbled godwit Limosa fedoa BCC O Year-round Beaches and coastal dunes Rhinoceros auklet (nesting colony

  2. Effect of Food, Diet and Nutrition on Military Readiness and Preparedness of Army Personnel and Dependents in a Peacetime Environment

    Science.gov (United States)

    1991-08-15

    rhinoceros ), and an omnivore (man). The data from these studies suggest that urinary levels of CHP is higher in animals consuming high levels of dietary...controlled two compartment black and white box, as well as the elevated plus maze was used to determine the stress/anxiety index following different diets. The...exploratory activity in the black and white chambers as well as the number of transitions were recorded in a 5 minute test session. The feeding of

  3. Arresting Tailhook: The Prosecution of Sexual Harassment in the Military

    Science.gov (United States)

    1993-04-01

    Secretary of the Navy (who reportedly visited some of the hospitality suites one of which had a rhinoceros phallic device dispensing white Russian drinks to...because it was overbroad. In the principal concurring opinion, Justice White stated that under the majority’s new "underinclusiveness" theory, "Title VII...attempted to explain that Title VII hostile environment sexual harassment need not fall under the rationale 15615of the opinion,. Justice White refuted

  4. Sea-Based X-Band (SBX) Radar Vessel Maintenance and Repair. Draft Environmental Assessment

    Science.gov (United States)

    2011-02-14

    recognizable of all the cetaceans. They have a striking black-and- white color pattern, and the adult male has a tall, erect dorsal fin (3.3 to 5.9 feet in...height). The white oval eye patch and variably-shaped saddle patch, in conjunction with the shape and notches in the dorsal fin, help in identifying...guillemot, common loon, harlequin duck, rhinoceros auklet, cormorants, scoters, and grebes. (National Wildlife Federation, 2010) Port Gardner Bay

  5. DSC of Milk Fats from Various Animals with High Levels of Medium ...

    African Journals Online (AJOL)

    The milk fats of elephant and white rhinoceros contain high amounts of 8:0, 10:0 and 12:0 which form triglyceride species that melt between 8 and 22 °C. The crystallographic behaviour of the milk lipids from blesbok and blue wildebeest differ from the other ruminant lipids, and that of horse and vervet monkey differ from the ...

  6. Transmission of Microsporidian Parasites of Mosquitoes.

    Science.gov (United States)

    1983-03-01

    milky- white coloration of the infected larvae when they are viewed against a dark background. The developmental sequence which leads to diploid spore...jar were examined for the white coloration due to an overt infection of Amblyospora sp. To check for transovarial infections, adult C. salinarius were...use of an indirect enzyme-linked immunosorbent assay to detect baculovirus in larvae and adults of Oryctes rhinoceros from Tonga J. Gen. Virol., 47

  7. JPRS Report, China.

    Science.gov (United States)

    1989-05-30

    animals such as wild horses, high-nosed antelope, rhinoceros , and the white -bottom monkey, which are almost extinct today; and of rare plants such as...similar background clearly tells us that there will be a new balance of power in Asia. With detente as a backdrop between the the United States... background ; he took part in anti-America aid Korea; he has been a worker; and he is more than 30 years old. The Chairman noticed him long ago, and

  8. Trypanosomosis and other co-infections in translocated black (Diceros bicornis michaeli) and white (Ceratotherium simum simum) rhinoceroses in Kenya

    OpenAIRE

    Obanda, V.; Kagira, J.; Chege, S.; Ouma, B.O.; Gakuya, F.

    2011-01-01

    Recent efforts towards the conservation of endangered rhinoceroses in Kenya include re-introduction of the animals into regions where they occurred previously. These efforts have however been hampered by mortalities of translocated animals. The current study was undertaken to determine the cause of deaths amongst black rhinoceroses (Diceros bicornis michaeli) and white rhinoceros (Ceratotherium simum simum), which were re-introduced into Meru National Park. Out of 28 translocated rhinoceroses...

  9. Busting the Bocage: American Combined Arms Operations in France, 6 June-31 July 1944

    Science.gov (United States)

    1988-11-01

    The combat capabilities of tanks and infantry were complementary. Prewar doctrine speci - fied that infantry-armor attack formations consisted of two...would endanger American lives. Unable to observe the enemy and to call fire on him from a safe dis- tance, infantrymen were deprived of field...tusks, troops called the device a "rhinoceros," and Shermans equipped with Culin’s invention became known as " rhino " tanks. Though the most famous of

  10. A CASE STUDY OF MODELING A TORUS IN DIFFERENT MODELING SOFTWARES

    OpenAIRE

    VELJKOVIĆ Milica; KRASIĆ Sonja; PEJIĆ Petar; TOŠIĆ Zlata

    2017-01-01

    Modeling of the complex geometric shapes requires the use of appropriate softwares. This study analyzes the process of modeling with two different computer softwares, AutoCAD and Rhinoceros. The aim is to demonstrate the similarities and differences between these softwares when used for modeling torus, a double curved geometric surface. The two modeling processes are compared in order to investigate the potentials of these softwares in the modeling of an architectural structure comprising a s...

  11. A serological survey of brucellosis in wild ungulate species from five game parks in Zimbabwe

    Directory of Open Access Journals (Sweden)

    Tatenda R. Motsi

    2013-09-01

    Full Text Available A retrospective serosurvey was carried out between 2009 and 2012 to detect antibodies to Brucella spp. in free-ranging African wildlife ungulates from five selected game parks in Zimbabwe. Samples were drawn from wildlife-livestock interface and non-interface areas in Zimbabwe. A total of 270 serum samples from four different species, namely African buffalo (Syncerus caffer (n=106, impala (Aepyceros melampus (n = 72, black rhinoceros (Diceros bicornis (n= 45 and white rhinoceros (Ceratotherium simum (n = 47, were tested. The percentage of positive samples was 17.0% in buffalo (18/106; 95% CI: 9.72% – 24.1% and 1.4% in impala (1/72; 95% CI: 0% – 4.2%. No antibodies to Brucella spp. were detected in the two rhinoceros species. The difference in the percentage of seropositive cases between buffalo and impala was significant (p< 0.05. Seropositivity to Brucella spp. was higher (19.1% in adult buffalo compared with juveniles and sub-adults younger than six years (5.9%. Further, seropositivity was marginally higher (20.4% in animals from wildlife-livestock interface areas than in those from non-interface areas (13.45%; OR = 1.45 although the difference was not statistically significant. The study showed that brucellosis could be more widespread in buffalo and may circulate in this species independently in the absence of contact with cattle, whilst rhinoceros may be considered less susceptible to brucellosis. The role of the wildlife-livestock interface in the epidemiology of brucellosis in wildlife and livestock is probably overstated but needs to be explored further.

  12. SNP discovery and characterisation in White Rhino (Ceratotherium simum) with application to parentage assignment

    Science.gov (United States)

    Labuschagne, Christiaan; Dalton, Desiré L.; Grobler, J. Paul; Kotzé, Antoinette

    2017-01-01

    Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs) and nine microsatellites (MS) in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the highest confidence level. This study thus provided us with a useful set of SNP and MS markers for parentage and relatedness testing. Further assessment of the utility of these markers over multiple (> three) generations and the incorporation of a larger variety of relationships among individuals (e.g. half-siblings or cousins) is strongly suggested. PMID:28170027

  13. SNP discovery and characterisation in White Rhino (Ceratotherium simum with application to parentage assignment

    Directory of Open Access Journals (Sweden)

    Christiaan Labuschagne

    Full Text Available Abstract The white rhino is one of the great success stories of modern wildlife conservation, growing from as few as 50-100 animals in the 1880s, to approximately 20,000 white rhinoceros remaining today. However, illegal trade in conservational rhinoceros horns is adding constant pressure on remaining populations. Captive management of ex situ populations of endangered species using molecular methods can contribute to improving the management of the species. Here we compare for the first time the utility of 33 Single Nucleotide Polymorphisms (SNPs and nine microsatellites (MS in isolation and in combination for assigning parentage in captive White Rhinoceros. We found that a combined dataset of SNPs and microsatellites was most informative with the highest confidence level. This study thus provided us with a useful set of SNP and MS markers for parentage and relatedness testing. Further assessment of the utility of these markers over multiple (> three generations and the incorporation of a larger variety of relationships among individuals (e.g. half-siblings or cousins is strongly suggested.

  14. Radar Satellite Imagery and Automatic Detection of Water Bodies : Radarski satelitski snimci i automatsko otkrivanje vodenih površina

    Directory of Open Access Journals (Sweden)

    Klemen Čotar

    2016-12-01

    Full Text Available System for mapping of water bodies in Slovenia and its immediate neighbourhood with Sentinel-1 radar satellites have implemented. Algorithms automatically detect presence of new data in the archive, download the data, analyse it, write the results, and upload them to a web portal. New acquisitions are currently available every six days, but this time will be halved when the second Sentinel-1 starts delivering the data. : Implementiran je sistem za kartiranje vodenih površina u Sloveniji i u neposrednoj blizini sa Sentinel-1 radarskim satelitima. Algoritmi automatski otkrivaju prisutnost novih podataka u arhivu, preuzimaju podatake, analiziraju, objavljuju rezultate, te ih prenose na web-portal. Nove akvizicije su trenutno dostupne svakih šest dana, ali ovaj puta će vrijeme biti prepolovljeno, kada drugi Sentinel-1 počne sa isporukom podataka.

  15. Kristološki naglasci govora o Božjem milosrđu u promišljanjima izabranih crkvenih otaca

    OpenAIRE

    Filić, Andrea

    2017-01-01

    Polazišno pitanje ovoga članka, nadahnuto tvrdnjama Waltera Kaspera, glasi: Ako milosrđe po definiciji u sebi nužno uključuje trpljenje može li se, dok držimo sigurnim da je netrpljivost jedna od bitnih vlastitosti božanske naravi, o njemu uopće govoriti, kako Kasper potiče, kao o temeljnom Božjem svojstvu i odrednici Božje biti? To se pitanje i odgovor na nj obrađuje pod kristološkim vidom i to na temelju promišljanja izabranih crkvenih otaca u čijim djelima pronalazimo govor o Božjem milosr...

  16. Utjecaj ekologije na dizajn ambalaže

    OpenAIRE

    Brčić, Ira

    2015-01-01

    Otpad je sve veći problem zaštite okoliša u cijelom svijetu, pa tako i kod nas. Zaštita okoliša bi trebala biti sastavni dio načina života, a ne obveza stoga bi trebalo odgajati i educirati djecu već od rane dobi da je zaštita okoliša, uključujući otpad nešto o čemu moramo brinuti i voditi računa. Udio ambalažnog otpada neprestano raste i postao je jedan od štetnijih utjecaja na okoliš. Nedostatkom informiranja potrošača raste i gomilanje otpada. Za minimiziranje negativnog utjecaja na oko...

  17. Edo Mihevc – urbanist, arhitekt in oblikovalec. Vrednotenje povojnega urbanizma slovenske obale

    Directory of Open Access Journals (Sweden)

    Jasna Kralj Pavlovec

    1999-01-01

    Full Text Available Edo Mihevc (1911–1985 je bil eden protagonistov slovenske moderne arhitekture po drugi svetovni vojni in eden Plečnikovih študentov, ki se je ločil od njegove manire ter zakorakal na pot samoizraznosti in iskanja skladja med modernizmom, regionalizmom in humanistično filozofijo. Pomen njegovega prispevka slovenski arhitekturi in kulturi je predstavljen skozi perspektivo večrazsežnostne dejavnosti, s poudarkom na vlogi urbanista slovenske obale. Posamezni deli iz njegovega opusa so predstavljeni z vidika časa nastanka, uporabe in arhitekturne vrednosti, pomen in vloga zamisli ter trendov pa skozi dela. V luči sodobnih doktrin varovanja kulturne dediščine, pravočasna ocena in minimalno varstvo njegove arhitekture mora biti doseženo, tudi za mojstrovine iz bližnje preteklosti.

  18. ŠTO JE TRENUTNO AKTUALNO U HRVATSKOM ŠUMARSTVU

    OpenAIRE

    HŠD, Uredništvo

    2017-01-01

    RIJEČ UREDNIŠTVAPriprema se novi Zakon o šumama, prema kojemu, nadamo se, neće biti opetovanog smanjivanja postotka naknade za općekorisne funkcije šuma. U vrijeme kada nam priroda daje kataklizmičke odgovore za našu nebrigu o njoj, a najveći svjetski zagađivači ne pristaju na njenu zaštitu, šumarskoj struci se već po običaju „sječe grana“ na kojoj, ne samo šumari, nego svi sjedimo. Sve više čuju se i glasovi koji sugeriraju izdvajanje priobalnog područja iz dosadašnjeg načina upravljanja, no...

  19. ANALIZA STABILNOSTI TRODIJELNIH TLAČNIH ŠTAPOVA

    OpenAIRE

    Vidoni, Luka

    2015-01-01

    Osnovni cilj ovoga rada je analizirati elastičnu stabilnost trodijelnih segmentnih štapova, čiji su presjeci različite krutosti na savijanje. Analiza će se provesti analitički i numerički. Na samom početku su definirani rubni uvjeti na temelju kojih će analizirati stabilnost jer o njima ovisi veličina kritične sile. Proračuni u obje analize rade se na temelju linearne teorije elastičnosti gdje će kratkim opisom biti objašnjena njena primjena. Analiza analitički se provodi korištenjem ...

  20. Kakovost v izobraževanju odraslih

    Directory of Open Access Journals (Sweden)

    Tanja Možina

    2000-12-01

    Full Text Available Zadnja desetletja znova in znova ponavljamo, da v sodobni, hitro spreminjajoči se družbi znanje ne more biti več le privilegij manjšine, ampak postaja vse bolj nujnost ter potreba za preživetje in normalno delovanje posameznika v družbeni skupnosti. Nova paradigma v izobraževanju, katere srž najdemo v potrebi po vseživljenjskem učenju, na področje izobraževanja odraslih vnaša nove razsežnosti in postavlja izobraževalce odraslih pred nove izzive ter odgovornosti.

  1. Rehabilitation of a Female Patient after Corrective Surgery for Tetralogy of Fallot

    Directory of Open Access Journals (Sweden)

    Cvetković Jovana

    2015-12-01

    Full Text Available Prikazana je bolesnica stara 25 godina sa tetralogijom Fallot. Ova urođena srčana mana nije tako česta, ali je najčešća među manama sa cijanozom. Lečenje bolesnica sa tetralogijom Fallot je hirurško i može biti palijativno i korektivno. Bolesnica koju smo prikazali imala je palijativnu operaciju, definitivnu korekciju i ponovnu operaciju zbog insuficijencije pulmonalne valvule. Tokom kardiovaskularne rehabilitacije bolesnica je bila ritmički stabilna uz odličnu toleranciju fizičkog napora. Imajući u vidu da se radi o mlađoj ženskoj osobi koja je tokom proteklih godina imala dva spontana abortusa, trudnoća nije apsolutno kontraindikovana, mada su spontani abortusi i komplikacije trudnoće češći kod ovih bolesnica.

  2. Žene u politici

    OpenAIRE

    Podnar, Ivana

    2017-01-01

    Kroz povijest žene su uvijek bile podcjenjivane i osporavane, oduvijek se smatralo da je njihov zadatak biti kod kuće, brinuti se oko odgoja djece i istovremeno obavljati teške fizičke poslove u kućanstvu. Kako se razvijalo društvo, dolazi i do određenih promjena u položaju žena. S vremenom, počele su dobivati različite poslove, društvena prava, pravo glasa, a u konačnici i pravo odlučivanja i rada u politici. Iako danas živimo u 21. stoljeću, postoje dijelovi svijeta u kojima žene...

  3. UTJECAJ STILOVA VODSTVA NA UPRAVLJANJE I ORGANIZACIJSKU KULTURU ŠUMARSKOG PODUZEĆA

    OpenAIRE

    LANDEKIĆ, Matija; ŠPORČIĆ, Mario; MARTINIĆ, Ivan; BAKARIĆ, Matija; LEPOGLAVEC, Kruno

    2016-01-01

    Menadžerski stil vođenja i odlučivanja uz proaktivnu organizacijsku kulturu, za šumarstvo u tranzicijskim zemljama, može biti ključna karika u procesu unapređenja poslovanja šumarskog poduzeća. U teoriji i praksi postoji više stilova vođenja, od autokratskog do demokratskog, a brojna su istraživanja dokazala postojanje uzročno-posljedične veze između stila rukovođenja i unapređenja poslovanja. U šumarstvu, međutim, nitko do sada nije ciljano vrednovao stil rukovođenja šumarskog poduzeća, te i...

  4. Problematika saopštavanja nekih književnih pojmova u italijanskim enciklopedijskim rečnicima

    Directory of Open Access Journals (Sweden)

    Milica Popović

    1976-12-01

    Full Text Available Enciklopedijski rečnik u jednoj svesci pored toga što je praktičan može biti i vrlo koristan, tj. izvanredan je posrednik. mnogostruke tematike koju saopštava na više manje koncizan način. Dve do tri hiljade stranica zbijenih, sitnih redova otkrivaju upečatljive pojedinosti iz bilo koje oblasti, tj. pružaju prvo neophodne osnovne priloge. Već u samom osnovnom prilogu saopštenja mogu biti dovoljno informativna, dovoljno iscrpna a često i sasvim zadovoljavajuča. U većini slučajeva odatle se mogu iscrpsti mnoge pojedinosti, čak i sa obiljem podataka, kao i definicije raznih pojmova, a često osnovni prilog može dovoljno da odredi ili potvrdi i koncepciju bilo koje tematike. Može da pruži i potrebnu preciznost možda kog vremenskog perioda, vrste roda, osobinu značenja i u prenosnom smislu, ili koju etimološku, istorijsku, umetničku, literarnu itd. funkciju datog pojma. Prema tome, uglavnom, i cenimo pravi izraz mere u kojoj se sistematičnost, kao glavna osobina enciklopedijskih saopštenja, pozitivno odražava. Medjutim, o efikasnosti i zadovoljavajućoj doslednosti materijala koji crpemo, razume se, možemo suditi u onom obimu do kojeg doseže poznavanje razvojne linije delatnosti koju pratimo.

  5. Microstructure, mechanical properties, castability and in vitro biocompatibility of Ti-Bi alloys developed for dental applications.

    Science.gov (United States)

    Qiu, K J; Liu, Y; Zhou, F Y; Wang, B L; Li, L; Zheng, Y F; Liu, Y H

    2015-03-01

    In this study, the microstructure, mechanical properties, castability, electrochemical behaviors, cytotoxicity and hemocompatibility of Ti-Bi alloys with pure Ti as control were systematically investigated to assess their potential applications in the dental field. The experimental results showed that, except for the Ti-20Bi alloy, the microstructure of all other Ti-Bi alloys exhibit single α-Ti phase, while Ti-20Bi alloy is consisted of mainly α-Ti phase and a small amount of BiTi2 and BiTi3 phases. The tensile strength, hardness and wear resistance of Ti-Bi alloys were demonstrated to be improved monotonically with the increase of Bi content. The castability test showed that Ti-2Bi alloy increased the castability of pure Ti by 11.7%. The studied Ti-Bi alloys showed better corrosion resistance than pure Ti in both AS (artificial saliva) and ASFL (AS containing 0.2% NaF and 0.3% lactic acid) solutions. The concentrations of both Ti ion and Bi ion released from Ti-Bi alloys are extremely low in AS, ASF (AS containing 0.2% NaF) and ASL (AS containing 0.3% lactic acid) solutions. However, in ASFL solution, a large number of Ti and Bi ions are released. In addition, Ti-Bi alloys produced no significant deleterious effect to L929 cells and MG63 cells, similar to pure Ti, indicating a good in vitro biocompatibility. Besides, both L929 and MG63 cells perform excellent cell adhesion ability on Ti-Bi alloys. The hemolysis test exhibited that Ti-Bi alloys have an ultra-low hemolysis percentage below 1% and are considered nonhemolytic. To sum up, the Ti-2Bi alloy exhibits the optimal comprehensive performance and has great potential for dental applications. Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

  6. Ovarian down Regulation by GnRF Vaccination Decreases Reproductive Tract Tumour Size in Female White and Greater One-Horned Rhinoceroses

    Science.gov (United States)

    Hermes, Robert; Schwarzenberger, Franz; Göritz, Frank; Oh, Serena; Fernandes, Teresa; Bernardino, Rui; Leclerc, Antoine; Greunz, Eva; Mathew, Abraham; Forsyth, Sarah; Saragusty, Joseph; Hildebrandt, Thomas Bernd

    2016-01-01

    Reproductive tract tumours, specifically leiomyoma, are commonly found in female rhinoceroses. Similar to humans, tumour growth in rhinoceroses is thought to be sex hormone dependent. Tumours can form and expand from the onset of ovarian activity at puberty until the cessation of sex-steroid influences at senescence. Extensive tumour growth results in infertility. The aim of this study was to down regulate reproductive function of tumour-diseased and infertile females to stop further tumour growth using a Gonadotropin releasing factor (GnRF) vaccine. Four infertile southern white (Ceratotherium simum simum) and three Greater one-horned rhinoceroses (rhinoceros unicornis) with active ovaries and 2.7 ± 0.9 and 14.0 ± 1.5 reproductive tract tumours respectively were vaccinated against GnRF (Improvac®, Zoetis, Germany) at 0, 4 and 16 weeks and re-boostered every 6–8 months thereafter. After GnRF vaccination ovarian and luteal activity was suppressed in all treated females. Three months after vaccination the size of the ovaries, the number of follicles and the size of the largest follicle were significantly reduced (Pwhite rhino: Pwhite rhinoceroses. In conclusion, GnRF vaccine effectively down regulated reproductive function and decreased the size of reproductive tract tumours in female rhinoceros. Our work is the first to use down regulation of reproductive function as a symptomatic treatment against benign reproductive tumour disease in a wildlife species. Nonetheless, full reversibility and rhinoceros fertility following GnRF vaccination warrants further evaluation. PMID:27403662

  7. Ovarian down Regulation by GnRF Vaccination Decreases Reproductive Tract Tumour Size in Female White and Greater One-Horned Rhinoceroses.

    Directory of Open Access Journals (Sweden)

    Robert Hermes

    Full Text Available Reproductive tract tumours, specifically leiomyoma, are commonly found in female rhinoceroses. Similar to humans, tumour growth in rhinoceroses is thought to be sex hormone dependent. Tumours can form and expand from the onset of ovarian activity at puberty until the cessation of sex-steroid influences at senescence. Extensive tumour growth results in infertility. The aim of this study was to down regulate reproductive function of tumour-diseased and infertile females to stop further tumour growth using a Gonadotropin releasing factor (GnRF vaccine. Four infertile southern white (Ceratotherium simum simum and three Greater one-horned rhinoceroses (rhinoceros unicornis with active ovaries and 2.7 ± 0.9 and 14.0 ± 1.5 reproductive tract tumours respectively were vaccinated against GnRF (Improvac®, Zoetis, Germany at 0, 4 and 16 weeks and re-boostered every 6-8 months thereafter. After GnRF vaccination ovarian and luteal activity was suppressed in all treated females. Three months after vaccination the size of the ovaries, the number of follicles and the size of the largest follicle were significantly reduced (P<0.03. Reproductive tract tumours decreased significantly in diameter (Greater-one horned rhino: P<0.0001; white rhino: P<0.01, presumably as a result of reduced sex-steroid influence. The calculated tumour volumes were reduced by 50.8 ± 10.9% in Greater one-horned and 48.6 ± 12.9% in white rhinoceroses. In conclusion, GnRF vaccine effectively down regulated reproductive function and decreased the size of reproductive tract tumours in female rhinoceros. Our work is the first to use down regulation of reproductive function as a symptomatic treatment against benign reproductive tumour disease in a wildlife species. Nonetheless, full reversibility and rhinoceros fertility following GnRF vaccination warrants further evaluation.

  8. A potential link between lateral semicircular canal orientation, head posture, and dietary habits in extant rhinos (Perissodactyla, Rhinocerotidae).

    Science.gov (United States)

    Schellhorn, Rico

    2018-01-01

    Extant rhinoceroses share the characteristic nasal horn, although the number and size of horns varies among the five species. Although all species are herbivores, their dietary preferences, occipital shapes, and common head postures vary. Traditionally, to predict the "usual" head posture (the most used head posture of animals during normal unstressed activities, i.e., standing) of rhinos, the occipital shape was used. While a backward inclined occiput implies a downward hanging head (often found in grazers), a forward inclined occiput is related to the horizontal head posture in browsing rhinos. In this study, the lateral semicircular canal (LSC) of the bony labyrinth was virtually reconstructed from µCT-images in order to investigate a possible link between LSC orientation and head posture in extant rhinoceroses. The usual head posture was formerly reconstructed for several non-rhinoceros taxa with the assumption that the LSC of the inner ear is held horizontal (parallel to the ground) during normal activity of the living animal. The current analysis of the LSC orientation resulted in a downward inclined usual head posture for the grazing white rhinoceros and a nearly horizontal head posture in the browsing Javan rhinoceros. The other three browsing or mixed feeding species show subhorizontal (closer to horizontal than a downgrade inclination) head postures. The results show that anatomical and behavioral aspects, like occipital shape, presence and size of horns/tusk-like lower incisors, as well as feeding and feeding height preferences influence the usual head posture. Because quantitative behavioral data are lacking for the usual head postures of the extant rhinos, the here described relationship between the LSC orientation and the resulting head posture linked to feeding preferences gives new insights. The results show, that the inner ear provides additional information to interpret usual head postures linked to feeding preferences that can easily be adapted

  9. Woolly rhino discovery in the lower Kolyma River

    DEFF Research Database (Denmark)

    Boeskorov, Gennady G.; Lazarev, Peter A.; Sher, Andrei V.

    2011-01-01

    A nearly complete frozen mummy of a woolly rhinoceros (Coelodonta antiquitatis Blum., 1799) was discovered in a gold mine on the lower reaches of the Kolyma River, north–eastern Siberia. This is the first find of the whole body of woolly rhino in permafrost. A large part of the mummified body...... was preserved, including the left part of the body, covered by skin, including skin of the head and ear, fore and hind legs. The skull with 2 horns and the lower jaw were also preserved. Most of the internal organs were lost, except the intestines, stomach, and their contents. A rib fragment from...

  10. Coprophilous ascomycetes in Kenya: Saccobolus species from wildlife dung

    Directory of Open Access Journals (Sweden)

    Mungai PG

    2012-03-01

    Full Text Available The taxonomy, occurrence and distribution of Saccobolus species was investigated from wild herbivore dung types in Kenya. Dung samples incubated in a moist chamber culture were examined for fungi over three months. Seven species, Saccobolus citrinus, S. depauperatus, S. diffusus, S. infestans, S. platensis, S. truncatus and S. versicolor were isolated from African elephant, black rhinoceros, Cape buffalo, dikdik, giraffe, hartebeest, hippopotamus, impala, waterbuck and zebra dung. Five taxa, S. citrinus, S. diffusus, S. infestans, S. platensis and S. truncatus, are new records for Kenya. The most common taxa were S. depauperatus and S. citrinus. The diversity of coprophilous Saccobolus species in wildlife dung is very high.

  11. Assessment of Biomarkers Associated with Joint Injury and Subsequent Post-Traumatic Arthritis

    Science.gov (United States)

    2015-10-01

    this burden to Department of Defense, Washington Headquarters Services , Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson...cartilage, are outlined from the DESS MR images using solid-modeling software (Rhinoceros, Robert NcNeel and Associates, Seattle, WA)4,5. Next, each...2664.70) 49593.27 (32925.18) 8516.75 (5800.99) 34858.84 (47077.89) 96916.98 (48965.4) SAA (ng/ml) 25100.46 (46440.13) 6195.64 (12390.13) 34131.26

  12. Timber Curtain

    DEFF Research Database (Denmark)

    2015-01-01

    Timber Curtain is a site specific architectural installation, which is part of a research project conducted at the Aarhus School of Architecture as collaboration between Ph.D. and associate professor Niels Martin Larsen and the author. Through a digitally scripted associative model we performed s...... of pinewood timber, machined with a CMS Antares 5-axis CNC router. The digital process generation and simulation was implemented with Rhinoceros 3D, Grasshopper and GHPython, and AlphaCAM was used for preparing the CNC-routing....

  13. Conservation et étude de la valeur nutritive des larves de ...

    African Journals Online (AJOL)

    nymphal qu'après 50 jours d'élevage. Les premières émergences d'imagos sont observées à partir du 94ème jour. L'extraction des lipides à l'hexane avec la méthode de Soxhlet indique que les larves de R. phoenicis ont des taux plus élevés d'acides gras que celles de O. rhinoceros, tandis que les taux de protéines sont ...

  14. An Analysis of China’s Information Technology Strategies and their Implication for US National Security

    Science.gov (United States)

    2006-06-01

    team of American advisers to South Vietnam to report on conditions there (December 1961 White Paper) and to assess possible future American...unification of China under the Qin state. Sun Tzu, a military leader for one of the warring states, who led troops with brass-tipped spears and rhinoceros -hide... White House and U.S. House of Representatives sites were also shut down. By the morning of May 5, over 1,400 U.S. 77 sites were shut down by the

  15. Parametric modelling design applied to weft knitted surfaces and its effects in their physical properties

    Science.gov (United States)

    Oliveira, N. P.; Maciel, L.; Catarino, A. P.; Rocha, A. M.

    2017-10-01

    This work proposes the creation of models of surfaces using a parametric computer modelling software to obtain three-dimensional structures in weft knitted fabrics produced on single needle system machines. Digital prototyping, another feature of digital modelling software, was also explored in three-dimensional drawings generated using the Rhinoceros software. With this approach, different 3D structures were developed and produced. Physical characterization tests were then performed on the resulting 3D weft knitted structures to assess their ability to promote comfort. From the obtained results, it is apparent that the developed structures have potential for application in different market segments, such as clothing and interior textiles.

  16. Ecological factors differentially affect mercury levels in two species of sympatric marine birds of the North Pacific

    International Nuclear Information System (INIS)

    Hipfner, J.M.; Hobson, K.A.; Elliott, J.E.

    2011-01-01

    In 2003 and 2004, we measured mercury concentrations and δ 15 N and δ 13 C values in the whole blood of adults of two species of seabirds, Cassin's auklet (Ptychoramphus aleuticus) and rhinoceros auklet (Cerorhinca monocerata), during their prelaying, incubation, and provisioning periods. We also collected whole blood from the offspring of both seabirds. Among prey items, δ 15 N values were higher in fish than in crustaceans, while δ 13 C did not vary systematically between prey types. Mercury concentrations in prey showed little relationship with either stable isotope. In the zooplanktivorous Cassin's auklet, year, reproductive stage, and δ 15 N and δ 13 C stable isotope values explained only 14% of the variation in mercury concentrations in adult blood, and none of these variables had a statistically significant effect. In contrast, these same variables explained 41% of the variation in mercury levels in the more piscivorous rhinoceros auklet, and all but δ 15 N values had statistically significant effects. Mercury concentrations in adult rhinoceros auklets were higher in 2003 than in 2004; higher prior to laying than during the incubation or provisioning periods; and increased with δ 13 C values - but in just one of two years. In both species, mercury concentrations were substantially higher in adults than in nestlings. Our results accord with previous studies in showing that mercury concentrations can vary among years, species and age classes, while the marked variation with reproductive stage is noteworthy because it is so rarely considered. Our results may help to explain the disparate conclusions of previous studies: while many factors influence mercury concentrations in marine predators, they apparently do so in a manner that defies easy characterization. We believe that there is a need for more studies that consider a range of physiological, ecological and behavioral factors that might affect mercury burdens in marine predators. - Research

  17. A CASE STUDY OF MODELING A TORUS IN DIFFERENT MODELING SOFTWARES

    Directory of Open Access Journals (Sweden)

    VELJKOVIĆ Milica

    2017-05-01

    Full Text Available Modeling of the complex geometric shapes requires the use of appropriate softwares. This study analyzes the process of modeling with two different computer softwares, AutoCAD and Rhinoceros. The aim is to demonstrate the similarities and differences between these softwares when used for modeling torus, a double curved geometric surface. The two modeling processes are compared in order to investigate the potentials of these softwares in the modeling of an architectural structure comprising a shell of the torus. After a detailed comparative analysis, the essential characteristics and shortcomings of these programs are emphasized and they were used to recommend the more appropriate one.

  18. İŞGÖREN SEÇİM SÜRECİNDEKİ KRİTİK FAALİYETLERİN ANALİTİK HİYERARŞİ SÜRECİ İLE DEĞERLENDİRİLMESİ

    Directory of Open Access Journals (Sweden)

    Nurcan TEMİZ

    2015-10-01

    Full Text Available ÖZ: Proje, zaman, kaynak ve para gibi kısıtları olan, belirli bir amacı başarmak için başlangıç ve bitiş sürelerine sahip ve öncelik ilişkileri olan bir takım faaliyetlerden oluşan bir süreçtir. İşgören seçimi her işletme için hayati bir öneme sahiptir. Bu süreç, işletmenin ihtiyaçlarına uygun işgücünü bulma ve işletmeye dahil etme faaliyetlerinden oluşan bir proje gibi düşünülebilir. Bu çalışmada bir fast food işletmesinin müdür adayı işe alım süreci incelenmiştir. İşgören seçim sürecinde işletme tarafından uygulanan faaliyetlerin öncelik ilişkileri kullanılarak proje planlama tekniklerinden Kritik Yol Yöntemi (CPM ile projenin şebekesi çizilmiş, tüm faaliyetler için en erken başlama, en erken bitiş, en geç başlama, en geç bitiş süreleri, toplam boşluklar ve serbest boşluklar hesaplanmış, kritik faaliyetler bulunmuştur. Daha sonra kritik faaliyetlerden bazılarının önceliklerini (göreli önemlerini bulmak için Analitik Hiyerarşi Süreci (AHS kullanılmıştır. ABSTRACT: Project is a process, which has restrictions such as time, resource and money, consisting of a set of activities that have starting and finishing time and precedency relations in order to achieve a specific purpose. Personnel selection has a vital importance for every business. This process can be considered as a project that consists of activities such as finding correct labour force and incorporating them to the organization. In this study personnel selection process of fast food organization was examined. By using precedence relationships of activities, pursued by organization in the personnel selection process, network of project is drawn via Critical Path Method (CPM, which is one of the project planning techniques, earliest start and earliest finish times, latest start and latest finish times, total and free float of all activities were calculated and critical activities were found. Then

  19. Generation of biomechanics three-dimensional image for development of projects of custom implants in titanium alloy; Geracao de imagem tridimensional biomecanica para desenvolvimento de projetos de implantes personalizados em liga de titanio

    Energy Technology Data Exchange (ETDEWEB)

    Gregolin, Rafael Ferreira, E-mail: rfgregolin@gmail.com [Universidade Federal da Grande Dourados (UFGD), Dourados, MS (Brazil); Zavaglia, Cecilia Amelia de Camargo [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil); Tokimatsu, Ruis Camargo; Joao Antonio Pereira [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Ilha Solteira, SP (Brazil)

    2014-07-01

    Three-dimensional modeling is an indispensable tool in manufacturing biomodels. Only after the development of a 3D design can be produced, by rapid prototyping, CNC machining, identical models of the areas of the human body. To perform computational numerical analysis is essential the creation of three-dimensional models. To Custom prostheses the use of the image of the deployment region for the development of customized implant is extremely important because it assists in the process. Only with the 3D design of the deployment region is achieved by testing and improving the designer of the prosthesis with great perfection. In the study presented here was developed a three-dimensional modeling of the jaw of a patient by computed tomography (CT) of the skull of the same . The Invesalius software was used, from the files generated by CT, to create a 3D picture of the skull. This image was exported in STL format for Rhinoceros® software to be cleaned, smoothed and separate the region of interest. After working in Rhinoceros® the image was transformed into a NURBS solid and saved in IGES format. This extension (IGES) was chosen to export the image to the Ansys Workbench® software and thus perform a static structural finite element analysis by applying own forces and fixations of human anatomy. The greatest stress found in the mandible was 213.59 MPa. (author)

  20. Species-specific responses of Late Quaternary megafauna to climate and humans

    Science.gov (United States)

    Lorenzen, Eline D.; Nogués-Bravo, David; Orlando, Ludovic; Weinstock, Jaco; Binladen, Jonas; Marske, Katharine A.; Ugan, Andrew; Borregaard, Michael K.; Gilbert, M. Thomas P.; Nielsen, Rasmus; Ho, Simon Y. W.; Goebel, Ted; Graf, Kelly E.; Byers, David; Stenderup, Jesper T.; Rasmussen, Morten; Campos, Paula F.; Leonard, Jennifer A.; Koepfli, Klaus-Peter; Froese, Duane; Zazula, Grant; Stafford, Thomas W.; Aaris-Sørensen, Kim; Batra, Persaram; Haywood, Alan M.; Singarayer, Joy S.; Valdes, Paul J.; Boeskorov, Gennady; Burns, James A.; Davydov, Sergey P.; Haile, James; Jenkins, Dennis L.; Kosintsev, Pavel; Kuznetsova, Tatyana; Lai, Xulong; Martin, Larry D.; McDonald, H. Gregory; Mol, Dick; Meldgaard, Morten; Munch, Kasper; Stephan, Elisabeth; Sablin, Mikhail; Sommer, Robert S.; Sipko, Taras; Scott, Eric; Suchard, Marc A.; Tikhonov, Alexei; Willerslev, Rane; Wayne, Robert K.; Cooper, Alan; Hofreiter, Michael; Sher, Andrei; Shapiro, Beth; Rahbek, Carsten; Willerslev, Eske

    2014-01-01

    Despite decades of research, the roles of climate and humans in driving the dramatic extinctions of large-bodied mammals during the Late Quaternary remain contentious. We use ancient DNA, species distribution models and the human fossil record to elucidate how climate and humans shaped the demographic history of woolly rhinoceros, woolly mammoth, wild horse, reindeer, bison and musk ox. We show that climate has been a major driver of population change over the past 50,000 years. However, each species responds differently to the effects of climatic shifts, habitat redistribution and human encroachment. Although climate change alone can explain the extinction of some species, such as Eurasian musk ox and woolly rhinoceros, a combination of climatic and anthropogenic effects appears to be responsible for the extinction of others, including Eurasian steppe bison and wild horse. We find no genetic signature or any distinctive range dynamics distinguishing extinct from surviving species, underscoring the challenges associated with predicting future responses of extant mammals to climate and human-mediated habitat change. PMID:22048313

  1. Rewinding the process of mammalian extinction.

    Science.gov (United States)

    Saragusty, Joseph; Diecke, Sebastian; Drukker, Micha; Durrant, Barbara; Friedrich Ben-Nun, Inbar; Galli, Cesare; Göritz, Frank; Hayashi, Katsuhiko; Hermes, Robert; Holtze, Susanne; Johnson, Stacey; Lazzari, Giovanna; Loi, Pasqualino; Loring, Jeanne F; Okita, Keisuke; Renfree, Marilyn B; Seet, Steven; Voracek, Thomas; Stejskal, Jan; Ryder, Oliver A; Hildebrandt, Thomas B

    2016-07-01

    With only three living individuals left on this planet, the northern white rhinoceros (Ceratotherium simum cottoni) could be considered doomed for extinction. It might still be possible, however, to rescue the (sub)species by combining novel stem cell and assisted reproductive technologies. To discuss the various practical options available to us, we convened a multidisciplinary meeting under the name "Conservation by Cellular Technologies." The outcome of this meeting and the proposed road map that, if successfully implemented, would ultimately lead to a self-sustaining population of an extremely endangered species are outlined here. The ideas discussed here, while centered on the northern white rhinoceros, are equally applicable, after proper adjustments, to other mammals on the brink of extinction. Through implementation of these ideas we hope to establish the foundation for reversal of some of the effects of what has been termed the sixth mass extinction event in the history of Earth, and the first anthropogenic one. Zoo Biol. 35:280-292, 2016. © 2016 The Authors. Zoo Biology published by Wiley Periodicals, Inc. © 2016 The Authors. Zoo Biology published by Wiley Periodicals, Inc.

  2. Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology

    Science.gov (United States)

    Lane, Emily P.; de Klerk-Lorist, Lin-Mari; Hofmeyr, Markus; van der Heijden, Elisabeth M. D. L.; Botha, Louise; van Helden, Paul; Miller, Michele; Buss, Peter

    2017-01-01

    Tuberculosis caused by Mycobacterium bovis is endemic in the African buffalo (Syncerus caffer) population in the Kruger National Park and other conservation areas in South Africa. The disease has been diagnosed in a total of 21 free ranging or semi-free ranging wildlife species in the country with highly variable presentations in terms of clinical signs as well as severity and distribution of tuberculous lesions. Most species are spillover or dead-end hosts without significant role in the epidemiology of the disease. White rhinoceroses (Ceratotherium simum) are translocated from the Kruger National Park in substantial numbers every year and a clear understanding of their risk to manifest overt tuberculosis disease and to serve as source of infection to other species is required. We report the findings of experimental infection of three white rhinoceroses with a moderately low dose of a virulent field isolate of Mycobacterium bovis. None of the animals developed clinical signs or disseminated disease. The susceptibility of the white rhinoceros to bovine tuberculosis was confirmed by successful experimental infection based on the ante mortem isolation of M. bovis from the respiratory tract of one rhinoceros, the presence of acid-fast organisms and necrotizing granulomatous lesions in the tracheobronchial lymph nodes and the detection of M. bovis genetic material by PCR in the lungs of two animals. PMID:28686714

  3. Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum: Susceptibility, clinical and anatomical pathology.

    Directory of Open Access Journals (Sweden)

    Anita L Michel

    Full Text Available Tuberculosis caused by Mycobacterium bovis is endemic in the African buffalo (Syncerus caffer population in the Kruger National Park and other conservation areas in South Africa. The disease has been diagnosed in a total of 21 free ranging or semi-free ranging wildlife species in the country with highly variable presentations in terms of clinical signs as well as severity and distribution of tuberculous lesions. Most species are spillover or dead-end hosts without significant role in the epidemiology of the disease. White rhinoceroses (Ceratotherium simum are translocated from the Kruger National Park in substantial numbers every year and a clear understanding of their risk to manifest overt tuberculosis disease and to serve as source of infection to other species is required. We report the findings of experimental infection of three white rhinoceroses with a moderately low dose of a virulent field isolate of Mycobacterium bovis. None of the animals developed clinical signs or disseminated disease. The susceptibility of the white rhinoceros to bovine tuberculosis was confirmed by successful experimental infection based on the ante mortem isolation of M. bovis from the respiratory tract of one rhinoceros, the presence of acid-fast organisms and necrotizing granulomatous lesions in the tracheobronchial lymph nodes and the detection of M. bovis genetic material by PCR in the lungs of two animals.

  4. Species-specific responses of Late Quaternary megafauna to climate and humans.

    Science.gov (United States)

    Lorenzen, Eline D; Nogués-Bravo, David; Orlando, Ludovic; Weinstock, Jaco; Binladen, Jonas; Marske, Katharine A; Ugan, Andrew; Borregaard, Michael K; Gilbert, M Thomas P; Nielsen, Rasmus; Ho, Simon Y W; Goebel, Ted; Graf, Kelly E; Byers, David; Stenderup, Jesper T; Rasmussen, Morten; Campos, Paula F; Leonard, Jennifer A; Koepfli, Klaus-Peter; Froese, Duane; Zazula, Grant; Stafford, Thomas W; Aaris-Sørensen, Kim; Batra, Persaram; Haywood, Alan M; Singarayer, Joy S; Valdes, Paul J; Boeskorov, Gennady; Burns, James A; Davydov, Sergey P; Haile, James; Jenkins, Dennis L; Kosintsev, Pavel; Kuznetsova, Tatyana; Lai, Xulong; Martin, Larry D; McDonald, H Gregory; Mol, Dick; Meldgaard, Morten; Munch, Kasper; Stephan, Elisabeth; Sablin, Mikhail; Sommer, Robert S; Sipko, Taras; Scott, Eric; Suchard, Marc A; Tikhonov, Alexei; Willerslev, Rane; Wayne, Robert K; Cooper, Alan; Hofreiter, Michael; Sher, Andrei; Shapiro, Beth; Rahbek, Carsten; Willerslev, Eske

    2011-11-02

    Despite decades of research, the roles of climate and humans in driving the dramatic extinctions of large-bodied mammals during the Late Quaternary period remain contentious. Here we use ancient DNA, species distribution models and the human fossil record to elucidate how climate and humans shaped the demographic history of woolly rhinoceros, woolly mammoth, wild horse, reindeer, bison and musk ox. We show that climate has been a major driver of population change over the past 50,000 years. However, each species responds differently to the effects of climatic shifts, habitat redistribution and human encroachment. Although climate change alone can explain the extinction of some species, such as Eurasian musk ox and woolly rhinoceros, a combination of climatic and anthropogenic effects appears to be responsible for the extinction of others, including Eurasian steppe bison and wild horse. We find no genetic signature or any distinctive range dynamics distinguishing extinct from surviving species, emphasizing the challenges associated with predicting future responses of extant mammals to climate and human-mediated habitat change. © 2011 Macmillan Publishers Limited. All rights reserved

  5. Generation of biomechanics three-dimensional image for development of projects of custom implants in titanium alloy

    International Nuclear Information System (INIS)

    Gregolin, Rafael Ferreira; Zavaglia, Cecilia Amelia de Camargo; Tokimatsu, Ruis Camargo; Joao Antonio Pereira

    2014-01-01

    Three-dimensional modeling is an indispensable tool in manufacturing biomodels. Only after the development of a 3D design can be produced, by rapid prototyping, CNC machining, identical models of the areas of the human body. To perform computational numerical analysis is essential the creation of three-dimensional models. To Custom prostheses the use of the image of the deployment region for the development of customized implant is extremely important because it assists in the process. Only with the 3D design of the deployment region is achieved by testing and improving the designer of the prosthesis with great perfection. In the study presented here was developed a three-dimensional modeling of the jaw of a patient by computed tomography (CT) of the skull of the same . The Invesalius software was used, from the files generated by CT, to create a 3D picture of the skull. This image was exported in STL format for Rhinoceros® software to be cleaned, smoothed and separate the region of interest. After working in Rhinoceros® the image was transformed into a NURBS solid and saved in IGES format. This extension (IGES) was chosen to export the image to the Ansys Workbench® software and thus perform a static structural finite element analysis by applying own forces and fixations of human anatomy. The greatest stress found in the mandible was 213.59 MPa. (author)

  6. Translocations as experiments in the ecological resilience of an asocial mega-herbivore.

    Directory of Open Access Journals (Sweden)

    Wayne L Linklater

    Full Text Available Species translocations are remarkable experiments in evolutionary ecology, and increasingly critical to biodiversity conservation. Elaborate socio-ecological hypotheses for translocation success, based on theoretical fitness relationships, are untested and lead to complex uncertainty rather than parsimonious solutions. We used an extraordinary 89 reintroduction and 102 restocking events releasing 682 black rhinoceros (Diceros bicornis to 81 reserves in southern Africa (1981-2005 to test the influence of interacting socio-ecological and individual characters on post-release survival. We predicted that the socio-ecological context should feature more prominently after restocking than reintroduction because released rhinoceros interact with resident conspecifics. Instead, an interaction between release cohort size and habitat quality explained reintroduction success but only individuals' ages explained restocking outcomes. Achieving translocation success for many species may not be as complicated as theory suggests. Black rhino, and similarly asocial generalist herbivores without substantial predators, are likely to be resilient to ecological challenges and robust candidates for crisis management in a changing world.

  7. Ironija i satira u dramskim djelima Maxa Frischa: Mehanizmi suočavanja s izazovima 20. stoljeća

    Directory of Open Access Journals (Sweden)

    Katarina Žeravica

    2014-12-01

    Full Text Available Irony and satire, two complex phenomena, find their respective places in the dramatic works of Max Frisch (1911–1991, a Swiss playwright and novelist and have gone through many changes gaining on different meanings, depending on the socio-cultural context, dominant literary and philosophical theories of a certain period of time in which they were analyzed and recognized as such. The aim of this paper is to show that Max Frisch, a 20th century intellectual who witnessed the major political, cultural and social changes of the second half of the 20th century in Europe and worldwide, uses irony and satire for coping with the reality and challenges of the time he lived in. Keywords: Max Frisch, dramatic work, irony, satire, 20th centuryNeutralna pozicija koju je Švicarska imala za vrijeme Drugoga svjetskog rata, a zbog koje je ostala pošteđena ratnih zbivanja te otvorenost klasičnim, ali i suvremenim europskim i svjetskim književnim te kazališnim utjecajima, stvorili su pozitivno ozračje za dobru, aktualnu i suvremenu švicarsku dramsku književnost na njemačkom jeziku nakon 1945. godine čiji predstavnik postaje jedan od najvećih švicarskih književnika, Max Frisch (1911–1991. U svojoj dugogodišnjoj književnoj karijeri Frisch je bio iznimno aktivan kao prozaist, napisavši pet romana, pet pripovijesti te četiri dnevnika, ali i kao dramatičar, ostavivši bogat dramski opus koji broji jedanaest dramskih ostvarenja napisanih između 1945. i 1978. godine. Upravo će Frischove drame biti predmetom ovoga rada, odnosno naglasak će biti na onima koje su se analizom pokazale najreprezentativnijima u cilju dokazivanja postojanja ironije i satire u istima kao mehanizmima suočavanja s izazovima koje je pred čovjeka-intelektualca stavilo 20. stoljeće, ali i kao sredstvima putem kojih Frisch progovara, kritizira i prokazuje besmisao jednog vremena kojemu je i sâm pripadao.

  8. IMPORTANCE OF AGRO-ECOLOGICAL AND ECONOMIC STRATEGIC MANAGEMENT WITHIN THE TERTIARY SECTOR

    Directory of Open Access Journals (Sweden)

    SLOBODAN POPOVIĆ

    2015-12-01

    Full Text Available The aim of this paper is to emphasize the importance of eco-agro-tourism within the tertiary sector economy countries, primarily in terms of the overall development of society as a whole. This question is particularly evident in terms of the Great Depression and every attempt to find out through improvement of business according to the author deserves attention. The authors make conclusions that agro significantly affects the rural areas in the states that are struggling to achieve development in all spheres of economy. In addition, the authors conclude that there is the impact of eco-agro-tourism and environmental and natural environment in which people live. The impact it has on the following levels such as: general, cultural, demographic, social, and economic, and others. Based on that clearly stands out as a multidisciplinary focus and the basis of observation of eco-agro-tourism. Race for financial gain often overlooked and natural environment in which BITIS people. Eco-agro-tourism includes a range of activities, services and additional facilities organized by the population mainly lives in the countryside or on family farms that have potential chances of developing structural attract tourists and generate income that are not standard. The authors conclude that organic agriculture can affect the development of the economy especially in rural areas, with it can to promote the concept of farm to be a new view to promote the development of approaches, such as: organic production, traditional crafts in order to increase the total supply, product sales to tourists that are manufactured on farm and more. Consequently, 159 Annals of the „Constantin Brâncuşi” University of Târgu Jiu, Economy Series, Issue 6/2015 „ACADEMICA BRÂNCUŞI” PUBLISHER, ISSN 2344 – 3685/ISSN-L 1844 - 7007 the authors' conclusion would be that the agro-tourism and eco-tourism have a chance to create a more substantial income people BITIS in the countryside

  9. Isaac Asimov: Kraj / S engleskog preveo Emil Šprljan

    Directory of Open Access Journals (Sweden)

    Isaac Asimov

    2011-12-01

    Full Text Available Mislite li da možemo naučiti živjeti tako da napustimo ideju o svijetu nakon smrti, o nepovredivosti majčinstva, svetosti spolnosti, zatrovanost nacionalizmom, žeđ za beskonačnom slobodom i poštovanje industrije u zamjenu za ograničenje rađanja sa svrhom očuvanja ljudske vrste pri čemu bi se seks upražnjavao za zabavu, a sve to podrazumijevalo bi postojanje svjetske vlade, kontroliranu ekologiju i obrazovanje iz zabave?I da sve to moramo učiniti prije isteka dvadesetog stoljeća?Pa, baš i ne moramo. Samo, ukoliko to ne učinimo, naša će civilizacija biti uništena za trideset godina. I to je sve.Između ostalog, po zanimanju sam prorok. To jest, predskazujem budućnost i plaćen sam za to.Naravno, postoji tu i jedna kvaka. Nisam varalica, stoga je moja korisnost i više nego upitna. Kako ne prelazim rukom preko kristalne kugle, ne pružam usluge pratitelja kroz svijet duhova, nemam dara za otkrivenja i potpuno sam lišen mistične intuicije, nikome ne mogu reći koji će konj pobijediti na utrci, ili vara li nekoga žena, ili koliko će živjeti.

  10. Izbor optimalnog puta za kretanje organizovanog kolonskog saobraćajnog toka na osnovu rezultata modeliranja / Choosing an optimal route for organized vehicle movement based on modeling results

    Directory of Open Access Journals (Sweden)

    Radomir S. Gordić

    2006-04-01

    Full Text Available U toku planiranja i praktične realizacije zadataka jedinica Vojske SCG često se javlja problem izbora optimalnog puta između dva mesta (čvora na putnoj mreži. Kriterijumi optimizacije mogu biti različiti. Ovaj projekat treba da omogući brzo i lako određivanje optimalnog puta, primenom dinamičkog programiranja (DP, uz korišćenje Belmanovog (Bellman, algoritma u zavisnosti od izabranog kriterijuma -parametra. Kriterijum optimizacije je minimalno vreme kretanja (putovanja, koje je dobijeno imitacionim modeliranjem kolonskog saobraćajnog toka. Razrađeni algoritam omogućuje izbor optimalnog puta, za bilo koja dva čvora na mreži. / During the planning and practical realization of Serbian & Montenegro units' tasks a problem -which often occurs is choosing an optimal transport route between two places (nodes. Optimization criteria can be various. This project should enable quick and easy defining of an optimal route, applying dynamic programing (DP using Bellman's algorithm depending on chosen criteria - parameter. Optimization criteria represent minimum movement time (traveling, which are taken from imitational modeling of a traffics queue flow. Operating algorithm enable choosing an optimal transport route, for any two nodes on a road map.

  11. The complementarity-determining region sequences in IgY antivenom hypervariable regions

    Directory of Open Access Journals (Sweden)

    David Gitirana da Rocha

    2017-08-01

    Full Text Available The data presented in this article are related to the research article entitled "Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da Rocha et al., 2017 [1]. Complementarity-determining region (CDR sequences are variable antibody (Ab sequences that respond with specificity, duration and strength to identify and bind to antigen (Ag epitopes. B lymphocytes isolated from hens immunized with Bitis arietans (Ba and anti-Crotalus durissus terrificus (Cdt venoms and expressing high specificity, affinity and toxicity neutralizing antibody titers were used as DNA sources. The VLF1, CDR1, CDR2, VLR1 and CDR3 sequences were validated by BLASTp, and values corresponding to IgY VL and VH anti-Ba or anti-Cdt venoms were identified, registered [Gallus gallus IgY Fv Light chain (GU815099/Gallus gallus IgY Fv Heavy chain (GU815098] and used for molecular modeling of IgY scFv anti-Ba. The resulting CDR1, CDR2 and CDR3 sequences were combined to construct the three - dimensional structure of the Ab paratope.

  12. Public health aspects of snakebite care in West Africa: perspectives from Nigeria.

    Science.gov (United States)

    Habib, Abdulrazaq G

    2013-10-17

    Snakebite envenoming is a major public health problem among rural communities of the Nigerian savanna. The saw-scaled or carpet viper (Echis ocellatus) and, to a lesser extent, the African cobras (Naja spp.) and puff adders (Bitis arietans) have proved to be the most important cause of mortality and morbidity. The main clinical features of E. ocellatus envenoming are systemic hemorrhage, incoagulable blood, shock, local swelling, bleeding and, occasionally, necrosis. Bites may be complicated by amputation, blindness, disability, disfigurement, mutilation, tissue destruction and psychological consequences. Antivenom remains the hallmark and mainstay of envenoming management while studies in Nigeria confirm its protection of over 80% against mortality from carpet-viper bites. However, the availability, distribution and utilization of antivenom remain challenging although two new antivenoms (monospecific EchiTab G and trispecific EchiTab ICP-Plus) derived from Nigerian snake venoms have proven very effective and safe in clinical trials. A hub-and-spoke strategy is suggested for broadening antivenom access to endemic rural areas together with instituting quality assurance, standardization and manpower training. With the advent of antivenomics, national health authorities must be aided in selecting and purchasing antivenoms appropriate to their national needs while manufacturers should be helped in practical ways to improve the safety, efficacy and potential coverage against snake venoms and pricing of their products.

  13. Kvalitet u funkciji maksimizacije borbene gotovosti / Quality in the function of combat readiness maximization

    Directory of Open Access Journals (Sweden)

    Nenko Brkljač

    2006-07-01

    Full Text Available U sistemu opremanja Vojske sredstvima NVO sistemu menadžmenta kvalitetom mora biti podređena čitava organizacija, kao i opredeljenje najvišeg rukovodstva i zaposlenih. Neophodni preduslovi za uspostavljanje efikasnog (racionalnog sistema sadržani su u primeni suvremenih pristupa obezbeđenju kvaliteta, koji isključuju eklektički pristup pitanju "dokumentovanosti", koja je samo jedan u nizu velikog broja elemenata (složenih procesa za obezbeđenje kvaliteta, koje treba oživotvoriti da bi se kreirala, kao krajnji rezultat, maksimalna borbena gotovost. / In order to achieve top combat readiness, it is necessary that military system provision top management recognize need for quality management system establishment based on 'process approach'. Whole organization and orientation of top management and employee must be subjected to quality management system. For efficient (rational quality management system consolidation it is necessary to assure contemporary approach quality assurance, excluding eclectic approach to 'documentation'. 'Documentation' is only one condition among many difficult elements (process for quality assurance which is necessary to create top combat readiness, as a final result.

  14. NMR structure of bitistatin – a missing piece in the evolutionary pathway of snake venom disintegrins.

    Science.gov (United States)

    Carbajo, Rodrigo J; Sanz, Libia; Perez, Alicia; Calvete, Juan J

    2015-01-01

    Extant disintegrins, as found in the venoms of Viperidae and Crotalidae snakes (vipers and rattlesnakes, represent a family of polypeptides that block the function of β1 and β3 integrin receptors, both potently and with a high degree of selectivity. This toxin family owes its origin to the neofunctionalization of the extracellular region of an ADAM (a disintegrin and metalloprotease) molecule recruited into the snake venom gland proteome in the Jurassic. The evolutionary structural diversification of the disintegrin scaffold, from the ancestral long disintegrins to the more recently evolved medium-sized, dimeric and short disintegrins, involved the stepwise loss of pairs of class-specific disulfide linkages and the processing of the N-terminal region. NMR and crystal structures of medium-sized, dimeric and short disintegrins have been solved. However, the structure of a long disintegrin remained unknown. The present study reports the NMR solution structures of two disulfide bond conformers of the long disintegrin bitistatin from the African puff adder Bitis arietans. The findings provide insight into how a structural domain of the extracellular region of an ADAM molecule, recruited into and selectively expressed in the snake venom gland proteome as a PIII metalloprotease in the Jurassic, has subsequently been tranformed into a family of integrin receptor antagonists. © 2014 FEBS.

  15. Zaštita računarskih mreža Ministarstva odbrane i Vojske Srbije primenom virtuelnog honeyneta / Security of computer network of the Ministry of Defence and the Serbian Armed Forces using virtual honeynets

    Directory of Open Access Journals (Sweden)

    Zoran Bobar

    2009-07-01

    Full Text Available U ovom radu obrađena je zaštita računarskih mreža u Ministarstvu odbrane i Vojsci Srbije primenom virtuelnog honeyneta. Zaštita je obrađena sa aspekta arhitekture računarskih mreža koje imaju pristup internetu. Predloženi koncept primene virtuelnog honeyneta uzima u obzir dostignuća nauke u ovoj oblasti u svetu, ostale primenjene metode i tehnike zaštite, mogućnosti i potrebe korisnika i elemente delova računarskog sistema Ministarstva odbrane i Vojske koji bi mogli biti meta napada sa udaljenih mesta globalne (internet mreže. / This paper covers the proposed solution for security of computer network in the Ministry of Defence and the Serbian Armed Forces using virtual honeynets. The security is covered from the aspect of the architecture of computer networks with Internet access. The proposed usage of virtual honeynets for protection takes into account the accomplishments of science in this field as well as security methods and techniques, users' needs and opportunities along with the computer network components of the MoD and the SAF that can be targets for attack.

  16. Razvoj nauke o održavanju tehničkih sistema / Development of the science of technical system maintenance

    Directory of Open Access Journals (Sweden)

    Jovan Todorović

    2003-03-01

    Full Text Available Sve veći zahtevi u pogledu upotrebnog kvaliteta i ekonomičnosti traže i stalno usavršavanje nauke o održavanju tehničkih sistema i odgovarajućih inženjerskih postupaka održavanja. Najveći značaj u ovom okviru imaju metode održavanja zasnovane na riziku, koje se poslednjih godina razvijaju, posebno za održavanje tehničkih sistema velike složenosti i velikih rizika. Očekuje se da će uskoro biti na raspolaganju i evropski standardi za održavanje na bazi rizika, koji će omogućiti veću raspoloživost, manje troškove održavanja i manje rizike ekonomskog, ekološkog i drugog karaktera. / Increasing demands for quality of service and cost-effectiveness require permanent improvement of the maintenance science and engineering methods for maintenance of complex engineering systems. Most significant are risk-based maintenance methods being developed over the last few years in particular for complex high risk systems. New European standards on risk-based maintenance will be soon available, providing a higher readiness, lower expenses and lower risks regarding economy, ecology and other influences.

  17. GENÇ ERİŞKİN TÜRK BİREYLERDE OKLÜZYON DÜZLEMİNİN ANATOMİK REFERANS DÜZLEM LERLE İLİŞKİSİ-THE RELATIONSHIP OF THE PLANE OF OCCLUSION TO ANATOMIC REFERENCE PLANES IN TURKISH YOUNG ADULTS

    OpenAIRE

    Bayraktar, Gülsen; Duran, Özlem; Fıratlı, Sönmez; Öztaş, Evren; Tufaner, Metin

    2013-01-01

    ÖzetOklüzyon düzleminin ön bölgede dudaklarla ilişkisi ve ön dişlerin doğru estetik konumları dikkate alınarak belirlenmesi birçok araştırmacının ortak görüşüdür. Ancak, bu düzlemin arka bölgedeki eğimi ve bitiş noktası konusunda değişik görüşler ileri sürülmektedir.Bu çalışmada, oklüzyon düzleminin FrankfurtÇalışmanın sonucunda elde edilen veriler, oklüzyon düzleminin incelenen diğer düzlemlerle ilişkisinin cinsiyete ve farklı toplumlara bağb olarak değişiklik gösterebileceği yönündedir.Anah...

  18. Comparative analyses of tooth wear in free-ranging and captive wild equids.

    Science.gov (United States)

    Taylor, L A; Müller, D W H; Schwitzer, C; Kaiser, T M; Castell, J C; Clauss, M; Schulz-Kornas, E

    2016-03-01

    Captive breeding has played a crucial role in the conservation of threatened equid species. Grazing ruminants and rhinoceros in captivity have less abrasion-dominated tooth wear than their free-ranging conspecifics, with potential negative consequences for their health. However, a similar study on wild equids in captivity is missing. The aim was to establish if different tooth wear patterns are exhibited by free-ranging and captive equids. Cross-sectional study of museum specimens comparing free-ranging and captive equids. Dental casts of maxillary cheek teeth of 228 museum specimens (122 from free-ranging and 106 from captive individuals) of 7 wild equid species were analysed using the extended mesowear method. Although teeth showing specific abnormalities were not scored, the presence of focal overgrowths (hooks) of the rostral premolars (106, 206) was recorded. Captive Equus ferus przewalskii, E. grevyi, E. hemionus, E. quagga boehmi and E. zebra hartmannae have less abrasion-dominated tooth wear on their premolars than their free-ranging conspecifics (P<0.001). Fewer differences were exhibited between populations in the molars. No differences were exhibited in the distal cusp of the molars (110, 210) between populations, except in a small sample of E. kiang. Captive equids exhibited more homogeneous wear along the tooth row whereas free-ranging equids exhibited a tooth wear gradient, with more abrasion on premolars than molars. There were more rostral hooks on the premolars (106, 206) in the captive than the free-ranging population (P = 0.02). Captive equids did experience less abrasion-dominated tooth wear than their free-ranging conspecifics, but the differences in tooth wear were less pronounced than those between captive and free-ranging wild ruminant and rhinoceros species. This indicates that feeding regimes for captive equids deviate less from natural diets than those for captive ruminants and rhinoceros but that factors leading to hook

  19. The Sarmatian vertebrates from Draxeni (Moldavian Platform

    Directory of Open Access Journals (Sweden)

    Vlad Codrea

    2007-10-01

    Full Text Available Middle Miocene (Sarmatian vertebrates had been unearthed at Draxeni (Vaslui district. The site is located in the northern area of the Moldavian Platform. There, the sand belonging to Şcheia Formation (Bessarabian is mined in a restricted open pit. This sand is related to a littoral environment (shoreface and foreshore. Some of its levels are rich in mollusc debris. Vertebrate remains, carried into the Bessarabian brackish basin are present too, but in smaller amounts. Mastodon, rhinoceros, hipparionine, tortoise remains had been collected there over several years. All teeth and bones are isolated and bear the marks of intensive rolling by waves and currents. This assemblage is typical for the top of Bessarabian in Moldavia, i.e. soon after the first hipparionine invasion in this part of the Europe. This assemblage can be related to the base of MN 9 unit.

  20. U-Th/ESR combined dating of faunal remains from the Mousterian open site of Beauvais (France)

    International Nuclear Information System (INIS)

    Michel, V.; Masaoudi, H.; Falgueres, Ch.; Yokoyama, Y.; Locht, J.L.; Antoine, P.

    1999-01-01

    Faunal remains from the Beauvais open site 'La Justice' (Oise) are the subject of an U-Th dating (disequilibrium of the uranium chain) and an ESR dating (electronic spin resonance). This study was performed in order to identify the chronological situation of the Middle Paleolithic levels of this site located in the north of France, supposedly aged stage 4 after stratigraphic correlations. U-Th ages of bones and dentine are between 20 and 200 ka and are scattered; however, the combined ESR/U-Th ages of rhinoceros dental enamels are homogeneous and indicate that the archaeological levels were deposited between 60 to 40 ka. This period corresponds to the end of the oxygen isotopic stage 4 to the beginning of stage 3. (authors) stage 3. (authors)

  1. Piroplasm parasites of white rhinoceroses (Ceratotherium simum in the Kruger National Park, and their relation to anaemia

    Directory of Open Access Journals (Sweden)

    D. Govender

    2011-04-01

    Full Text Available As part of a larger survey to map the geographical distribution of Babesia and Theileria parasites in the southern African rhinoceros population, white rhinoceroses were sampled during routine immobilisations in the Kruger National Park. Polymerase chain reaction (PCR and reverse line blot (RLB hybridisation assays were used to screen for the presence of piroplasms and complete blood counts were used to assess associated changes in clinical parameters. Of the 195 rhinoceroses sampled, 71 (36.4 % tested positive for the presence of Theileria bicornis, with no significant change in the haematological parameters measured, while 18 (9.2 % tested positive for Theileria equi. None of the rhinoceroses sampled tested positive for Babesia bicornis, a parasite associated with mortalities in black rhinoceroses.

  2. Investigations of astrophysically interesting nuclear reactions by the use of gas target techniques

    Energy Technology Data Exchange (ETDEWEB)

    Hammer, J.W. [Inst. fuer Strahlenphysik, Univ. Stuttgart, Stuttgart (Germany)

    1998-06-01

    A brief review of the common properties of windowless and recirculating gas targets is presented. As example the Stuttgart gas target facility Rhinoceros in the extended and in the supersonic jet mode with its properties and techniques is explained, also with respect to gas purification techniques. Furthermore several typical experiments from the field of nuclear astrophysics with characteristic results are described (D({alpha},{gamma}){sup 6}Li, {sup 15}N({alpha},{gamma}){sup 19}F, {sup 16}O(p,{gamma}){sup 17}F, {sup 16}O({alpha},{gamma}){sup 20}Ne, {sup 20}Ne({alpha},{gamma}){sup 24}Mg, {sup 21}Ne({alpha},n){sup 24}Mg, {sup 18}O({alpha},n){sup 21}Ne, {sup 17}O({alpha},n){sup 20}Ne). In several cases the experimental sensitivity could be raised by up to a factor of 10{sup 6}. (orig.)

  3. Steppic environments at the end of the upper pleistocene in southern Tunisia (Oued el Akarit)

    Science.gov (United States)

    Brun, Annik; Guerin, Claude; Levy, Alain; Riser, Jean; Rognon, Pierre

    The cross section of the Oued el Akarit, 25 km North of Gabès, is of considerable importance for the paleoenvironmental reconstitution of South Tunisia at the end of the Upper Pleistocene. Grey or black silts and clays and greenish gray sands which overlap them, contain many paleontological remains: rodents, birds and Rhinoceros bones, Gasteropods, Foraminifera, Ostracoda and pollen. All these sedimentological, faunistic or botanical indications allow to consider that this area, between approximatively 35,000 and 22,000 yr. B.P., belonged to the steppe domaine. But this steppe was probably more dense and more diversified than the present-day steppe which receives 170 mm of rain (annual average). This conclusion is in good agreement with the other paleoenvironmental data from the Chott Djerid, the Matmata plateau or the Gulf of Gabès cores, areas situated at about 100 km to the West, the South or the East of the Oued el Akarit.

  4. Analysis of complete mitochondrial genomes from extinct and extant rhinoceroses reveals lack of phylogenetic resolution

    DEFF Research Database (Denmark)

    Willerslev, Eske; Gilbert, Tom; Binladen, Jonas

    2009-01-01

    reconstruction of the rhinoceros phylogeny. While the six species cluster into three strongly supported sister-pairings: (i) The black/white, (ii) the woolly/Sumatran, and (iii) the Javan/Indian, resolution of the higher-level relationships has no statistical support. The phylogenetic signal from individual......BACKGROUND: The scientific literature contains many examples where DNA sequence analyses have been used to provide definitive answers to phylogenetic problems that traditional (non-DNA based) approaches alone have failed to resolve. One notable example concerns the rhinoceroses, a group for which...... genes is highly diffuse, with mixed topological support from different genes. Furthermore, the choice of outgroup (horse vs tapir) has considerable effect on reconstruction of the phylogeny. The lack of resolution is suggestive of a hard polytomy at the base of crown-group Rhinocerotidae...

  5. Perissodactyla diet

    Science.gov (United States)

    Schoenecker, Kathryn A.

    2018-01-01

    Perissodactyla (Schoch 1989) includes tapirs, rhinoceros, wild asses, horses, and zebras. It is the order of hoofed mammals referred to as “odd-toed ungulates” because its members have one to three weight-bearing toes and walk on hoofs or “ungules.” They are herbivores that are specialized to exploit grasslands and brushy habitat (rhinos, horses, asses, zebras) or dense tropical forests (tapirs). All share a common digestive system called hindgut fermentation, or cecal digestion (in the cecum), and can consume relatively tough, coarse forage. Some perissodactyls are “browsers” that forage primarily on woody shrubs and trees, whereas others are “grazers” with a graminoid-dominated diet. They are all predominantly opportunistic feeders and select for quantity over quality of forage; that is, they consume more abundant low-quality forage instead of searching and selecting for higher-quality forage because it gives them the advantage of reducing search effort, which conserves energy.

  6. The complete mitochondrial genome of horned Golden-line barbell, Sinocyclocheilus rhinocerous (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Peng, Shengfa; Lu, Jiang; Yang, Junxing; Chen, Xiaoli; Shi, Qiong

    2017-03-01

    The horned Golden-line barbel, Sinocyclocheilus rhinocerous (Cypriniformes, Cyprinidae) is a species of ray-finned fish of the genus Sinocyclocheilus with specific features. For example, its forehead protrudes forward like a rhinoceros horn. Here, we first reported the complete mitochondrial genome of this semi-caved teleost. The whole mitochondrial genome was 16 588 bp, and composed of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a 933 bp control region. A phylogenetic tree with the complete mitochondrial genome sequences of S. rhinocerous together with 10 other affinis species was constructed. The newly achieved mitochondrial genome sequence seem to be useful for addressing taxonomic issues and studying related evolution events, which would contribute to enrich the fish mitochondrial genome resource and promote the biological research.

  7. Status Identification and Prediction of Kaziranga-Karbi Anglong Wildlife Corridor of Assam, India, Using Geospatial Technology

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    Sharma Bhavna

    2014-11-01

    Full Text Available In the present study, an attempt has been made to discover the impacts of various developmental activities on the Kaziranga-Karbi Anglong wildlife corridor of Assam, India, using geospatial technology; as well as to predict the future status of the wildlife corridor by using the Cellular Automata Markov Model. Due to various anthropogenic activities the condition of the natural corridor has deteriorated, and in recent years many wild animals have been killed by road traffic accidents; in particular, greater one-horned (Indian rhinoceros (Rhinoceros unicornis are killed indiscriminately by the poachers, having been deviated from their regular routes. Changes were evident during the two decades between 1990 and 2010, when a large number of dense forest areas were converted to open forest, combined with losses of areas of scrub and marshy land. The area under agriculture and plantation crop increased along with the grassland during the decades. It has been found that the forests in Kaziranga-Karbi Anglong corridor are fragmented, and the area within the corridor is shrinking. There is considerable increase in patchiness, proportion of edge, and a perforated reduction of core areas within the corridor. The predicted land use/cover map of Kaziranga-Karbi Anglong corridor shows expansion of agricultural land, as well as plantation areas. It is estimated that only 25.66 percent of the present dense forest and 20.72 percent of open forest will remain by 2030, while areas under agriculture and plantation will increase by 33.91 and 5.33 percent, respectively.

  8. Tremors in white rhinoceroses (Ceratotherium simum during etorphine–azaperone immobilisation

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    Stephanie S. de Lange

    2017-02-01

    Full Text Available Little is known about the mechanisms causing tremors during immobilisation of rhinoceros and whether cardiorespiratory supportive interventions alter their intensity. Therefore, we set out to determine the possible mechanisms that lead to muscle tremors and ascertain whether cardiorespiratory supportive interventions affect tremor intensity. We studied tremors and physiological responses during etorphine–azaperone immobilisation in eight boma-held and 14 free-living white rhinoceroses. Repeated measures analysis of variance and a Friedman test were used to determine differences in variables over time and between interventions. Spearman and Pearson correlations were used to test for associations between variables. Tremor intensity measured objectively by activity loggers correlated well (p < 0.0001; r2 = 0.9 with visual observations. Tremor intensity was greatest when animals were severely hypoxaemic and acidaemic. Tremor intensity correlated strongly and negatively with partial pressure of oxygen (PaO2 (p = 0.0003; r2 = 0.9995 and potential of hydrogen (pH (p = 0.02, r2 = 0.97. It correlated strongly and positively with adrenaline concentrations (p = 0.003; r2 = 0.96, and adrenaline correlated strongly and negatively with PaO2 (p = 0.03; r2 = 0.95 and pH (p = 0.03; r2 = 0.94. Therefore, hypoxaemia and acidaemia were likely associated with the intensity of tremors through their activation of the release of tremorgenic levels of adrenaline. Tremors can be reduced if circulating adrenaline is reduced, and this can be achieved by the administration of butorphanol plus oxygen insufflation. Furthermore, to assist with reducing the risks associated with rhinoceros immobilisation, tremor intensity could be used as a clinical indicator of respiratory and metabolic compromise.

  9. Human-Wildlife Conflicts in Nepal: Patterns of Human Fatalities and Injuries Caused by Large Mammals.

    Science.gov (United States)

    Acharya, Krishna Prasad; Paudel, Prakash Kumar; Neupane, Prem Raj; Köhl, Michael

    2016-01-01

    Injury and death from wildlife attacks often result in people feeling violent resentment and hostility against the wildlife involved and, therefore, may undermine public support for conservation. Although Nepal, with rich biodiversity, is doing well in its conservation efforts, human-wildlife conflicts have been a major challenge in recent years. The lack of detailed information on the spatial and temporal patterns of human-wildlife conflicts at the national level impedes the development of effective conflict mitigation plans. We examined patterns of human injury and death caused by large mammals using data from attack events and their spatiotemporal dimensions collected from a national survey of data available in Nepal over five years (2010-2014). Data were analyzed using logistic regression and chi-square or Fisher's exact tests. The results show that Asiatic elephants and common leopards are most commonly involved in attacks on people in terms of attack frequency and fatalities. Although one-horned rhinoceros and bears had a higher frequency of attacks than Bengal tigers, tigers caused more fatalities than each of these two species. Attacks by elephants peaked in winter and most frequently occurred outside protected areas in human settlements. Leopard attacks occurred almost entirely outside protected areas, and a significantly greater number of attacks occurred in human settlements. Attacks by one-horned rhinoceros and tigers were higher in the winter, mainly in forests inside protected areas; similarly, attacks by bears occurred mostly within protected areas. We found that human settlements are increasingly becoming conflict hotspots, with burgeoning incidents involving elephants and leopards. We conclude that species-specific conservation strategies are urgently needed, particularly for leopards and elephants. The implications of our findings for minimizing conflicts and conserving these imperiled species are discussed.

  10. One sententional model with the prepositional accusativ AS proleptic subject

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    Antonić Ivana

    2010-01-01

    copulative-adjectival structure biti siguran (be certain, biti ubeđen (be assured and the verbs verovati (believe, misliti (think; then the verb of fictitious cognition uobražavati (imagine; the verbs of speaking pričati (talk and, used in that meaning, the verbs čuti (hear, čitati (read, then kazati (say, šuškati ‘speak quietly’ (whisper, sumnjati (doubt, nagađati (suspect; and at the end the verbs of fear: brinuti se ‘fear’ (worry, bojati se (fear, pribojavati se (have fears, plašiti se (fear, strepeti (fear for, strahovati (be apprehensive. Since with these verbs the complement proposition acquires various features when it comes to factivity, this sentential model is analyzed from that perspective, too. Finally, the paper also discusses the frequency of negative / positive characterization of the referent of the proleptic subject.

  11. Unfolded Protein Response Is Activated in the Hearts of Catecholaminergic Polymorphic Ventricular Tachycardia (Cpvt Mice

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    Bakiu Rigers

    2014-10-01

    Full Text Available Izoforma 2 kalsekvestrina (CSQ2 je glavni kalcijum-vezujući protein sarkoplazmatskog retikuluma (SR i nalazi se kako u srčanom tako i u skeletnom mišiću. CSQ2 deluje kao kalcijumski receptor koji reguliše oslobađanje Ca2+ jona iz SR, putem interakcije sa triadinom, junktinom i rianodinskim receptorom. Različite mutacije csq2 gena mogu da izazovu poremećaje u oslobađanju Ca2+ i time kontraktilne funkcije, čime doprinose ravoju aritmija i iznenadnoj srčanoj smrti mladih osoba koje boluju od kateholaminergičke polimorfne ventrikularne tahikardije (CPVT. Razvojem transgenetskih miševa sa CSG2 point mutacijom (R33Q i CPVT-om, primećen je drastičan pad nivoa mutiranog proteina. Prateći biomolekularni pristup, nekoliko analiza je izvedeno, koristeći tretman različitim antitelima, sa ciljem da se otkrije kada počinje smanjenje nivoa CSQ2, rasvetli mehanizam uključen u redukciju CSQ2 i ispita da li prisustvo mutiranih proteina utiče i na druge proteine. Rezultati ove studije su pokazali da se nivoi mutiranih CSQ2 smanjuju ubrzo nakon rodjenja, što je udruženo sa smanjenim nivoom ostalih značajnih proteina SR, uključujući triadin (TD. Takođe je primećeno da odgovor nesavijenih proteina može biti povezan sa ushodnom regulacijom proteina i aktivacijom ATF-6 zavisnog signalnog puta. Prisustvo R33Q mutacije je izazvalo smanjenje nivoa CSQ2 putem aktivacije odgovora nesavijenih proteina i posledične proteozomalne degradacije.

  12. Određivanje funkcije pouzdanosti motornih vozila kao složenog tehničkog sistema / Determination of the reliability function of motor vehicles as complex systems

    Directory of Open Access Journals (Sweden)

    Rade Guberinić

    2009-04-01

    Full Text Available Kod motornih vozila se, kao posebno značajne promene stanja, posmatraju promene performansi vozila zavisno od njegove starosti, odnosno puta koje je prešlo. Pri razmatranju radnih opterećenja motornih vozila i njihovih sastavnih elemenata, obično se smatra da stvarna radna opterećenja mogu biti mehanička, toplotna i strukturna, što usmerava pažnju na uzroke promena stanja. Polazni kriterijum za ocenu eksploatacionih pokazatelja pouzdanosti i gotovosti motornih vozila predstavlja frekvencija pojava stanja 'u otkazu'. Svi ostali kriterijumi nastaju matematičkom transformacijom ovog kriterijuma. Korišćenjem dobijenih podataka, primenom programskog paketa PROEFI, određeni su parametri za tri posmatrane raspodele: normalnu, eksponencijalnu i Vejbulovu. Nakon određivanja parametara pojedinih raspodela izvršen je izbor optimalne raspodele primenom testa Kolmogorov-Smirnova za stepen značajnosti od 0,20. / Changes in vehicle performances represent important changes of the overall condition of a vehicle and are analyzed regarding its life, ie. its mileage. Since real operational loads are generally considered to be mechanical, temperature-based and structural, the analysis of operational loads of motor vehicles and their elements focuses on the causes of condition changes. The initial criterion for estimating the exploitation reliability and readiness factors of motor vehicles is the frequency of 'in failure' condition occurrence. All other criteria result from the mathematical transformation of this criterion. The obtained data and the PROEFI software package have been used for determining the parameters of three observed distributions: normal, exponential and Veibull. An optimal distribution has been chosen using the Kolmogorov-Smirnof test for a significance level of 0.20.

  13. Projekt języka czarnogórskiego

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    Jerzy Molas

    2015-09-01

    Full Text Available Draft of the Montenegrin language The authors of the grammar of the Montenegrin language (Gramatika crnogorskoga jezika, Podgorica 2010 have constructed a linguistic system which definitely stands apart from other standards which grew out from the Shtokavian dialects. Such elements as phonemes |ś| and |ź|, potencijal imperfekta, the negative form of the verb biti, the doublets of the forms of the verb htjeti // šćeti, lexemes with results of iotacismes make up – against the Bosnian, Croatian, and Serbian languages – a separate system both in terms of quality and quantity: potentially they are so frequent that would radically change the picture of Montenegrin texts with regard to the “classic” Shtokavian languages/dialects. In addition, the authors have managed to not go too far and have avoided a temptation to introduce into the system more specific characteristics, which have not been used by the modern language for a long time, such as, for example, the expanded form of the adjectival conjugation of the Njegošowski type: momci prsih vatrenijeh czy raniš od Vidova dana junačkijem i konjskijem mesom gavranove i mrke vukove! or phoneme |з|. In addition, it would also be difficult to specify the elements, which would prove unequivocally the tendency of the proposed Montenegrin standard becoming similar to one of the remaining Shtokavian languages. The weakness of the project is its potential nature. In case of the grammar, one cannot speak about the descriptive nature thereof. It is a proposal of the norm and the standard, which can genuinely come into existence solely given the support of the political elites and on the basis of their introduction into the educational and administrative system, as well as – which is most probably the most important and the most difficult aspect – given the goodwill of the decisive majority of the users of the Montenegrin language, which at the current point is rather out of the question.

  14. Sklapanje ugovora o vezu i ugovora o ostavi plovila konkludentnim činima : [prikaz presude

    Directory of Open Access Journals (Sweden)

    Skorupan Wolff, Vesna

    2017-12-01

    Full Text Available Tužitelj kao vlasnik plovila smatra da je između njega i marine kao tuženika sklopljen konkludentnim činima ugovor o ostavi plovila. Tužitelj je kupio i preuzeo plovilo od ranijeg vlasnika koji je s marinom bio u ugovornom odnosu. Nakon što je tužitelj pozvan od tuženika (marine da preuzme i ukloni plovilo sa veza jer će u protivnom biti premješteno na drugi vez, tužitelj to nije učinio. Ne može se smatrati da je ugovor o ostavi bio zaključen, kako to smatra tužitelj, konkludentnim činima tužitelja koji nije preuzeo plovilo nakon određenog roka te tuženika koji je premjestio plovilo na drugi vez, a u zimskom razdoblju ga ponovno vratio na siguran zaštićeni dio marine. U ponašanju tuženika i njegovom pozivu tužitelju da preuzme plovilo te premještanju plovila na drugi manje siguran vez nakon što tužitelj u određenom roku nije preuzeo plovilo, nema elemenata izražavanja volje za sklapanje ugovora o čuvanju plovila. Nedostatak volje za sklapanja ugovora o ostavi već na strani tuženika ukazuje da između stranaka nije nastao ugovor o čuvanju tužiteljevog plovila.

  15. Prometni koridorji in poselitev v regiji. Navezovanje poselitve na javni promet

    Directory of Open Access Journals (Sweden)

    Mojca Sašek Divjak

    2004-01-01

    Full Text Available Navezovanje poselitve na javni promet na regionalni in mestni ravni je osnovna vsebina tega prispevka. Kot je značilno za zahodno Evropo, se tudi v Sloveniji v širših zaledjih večjih mest pojavljajo izraziti poselitveni pritiski in problemi, ki so vezani na razvoj suburbanizacije. V območjih regionalnih središč, kjer so močni prometni tokovi s pogostimi zastoji motornega prometa, bi moral biti javni promet osnova prometnega sistema. Pomemben je tudi pri konsolidaciji večjih gravitacijskih območij, zlasti somestij. Vendar pa ugotavljamo, da vzporedno s povečano rabo osebnega avtomobila upada uporaba javnih transportnih sredstev. Zato sedanje stanje zahteva sanacijo transportnih sistemov in primerno zgostitev poselitve, vezano predvsem na razvoj javnega prometa. To pa lahko dosežemo le s sinergetskim povezovanjem razvoja javnega potniškega prometa in prostorskega načrtovanja v trajnostno usmerjen poselitveni sistem. Na območju ljubljanske funkcijske regije smo še posebej obdelali povezovanje poselitve z regionalnim sistemom javnega prometa, predvsem s predvideno regionalno lahko železnico oziroma tramvajskim sistemom na ožjem mestnem območju. Predstavljen je model decentralizirane zgostitve poselitve. Na osnovi študije o konceptu razvoja naselij v koridorjih tirnega prometa je bil narejen predlog za potencialne možnosti zgostitve poselitve v ožjih območjih postaj primestne železnice v severnem delu regije od Črnuč do Kamnika.

  16. The effects of solvent on photocatalytic properties of Bi2WO6/TiO2 heterojunction under visible light irradiation

    Science.gov (United States)

    Guo, Qiyao; Huang, Yunfang; Xu, Hui; Luo, Dan; Huang, Feiyue; Gu, Lin; Wei, Yuelin; Zhao, Huang; Fan, Leqing; Wu, Jihuai

    2018-04-01

    Bi2WO6/TiO2 heterojunction photocatalysts with two different microstructures were controllably fabricated via a facile two-step synthetic route. XRD, XPS, SEM, TEM, BET-surface, DRS, PL spectra, photoelectrochemical measurement (Mott-Schottky), and zeta-potential analyzer were employed to clarify structural and morphological characteristics of the obtained products. The results showed that Bi2WO6 nanoparticles/nanosheets grew on the primary TiO2 nanorods. The TiO2 nanorods used as a synthetic template inhibit the growth of Bi2WO6 crystals along the c-axis, resulting in Bi2WO6/TiO2 heterostructure with one-dimensional (1D) morphology. The photocatalytic properties of Bi2WO6/TiO2 heterojunction photocatalysts were strongly dependent on their shapes and structures. Compared with bare Bi2WO6 and TiO2, Bi2WO6/TiO2 composite have stronger adsorption ability and better visible light photocatalytic activities towards organic dyes. The Bi2WO6/TiO2 composite prepared in EG solvent with optimal Bi:Ti ratio of 2:12 (S-TB2) showed the highest photocatalytic activity, which could totally decompose Rhodamine B within 10 min upon irradiation with visible light (λ > 422 nm), and retained the high photocatalytic performance after five recycles, confirming its stability and practical usability. The results of PL indicated that Bi2WO6 and TiO2 could combine well to form a heterojunction structure which facilitated electron-hole separation, and lead to the increasing photocatalytic activity.

  17. Zaštita računarskih mreža / Protection of computer networks

    Directory of Open Access Journals (Sweden)

    Milojko Jevtović

    2005-09-01

    Full Text Available U radu su obrađene metode napada, oblici ugrožavanja i vrste pretnji kojima su izložene računarske mreže, kao i moguće metode i tehnička rešenja za zaštitu mreža. Analizirani su efekti pretnji kojima mogu biti izložene računarske mreže i informacije koje se preko njih prenose. Opisana su određena tehnička rešenja koja obezbeđuju potreban nivo zaštite računarskih mreža, kao i mere za zaštitu informacija koje se preko njih prenose. Navedeni su standardi koji se odnose na metode i procedure kriptozaštite informacija u računarskim mrežama. U radu je naveden primer zaštite jedne lokalne računarske mreže. / In this paper different methods of attacks, threats and different forms of dangers to the computer networks are described. The possible models and technical solutions for networks protection are also given. The effects of threats directed to the computer networks and their information are analyzed certain technical solutions that provide necessary protection level of the computer networks as well as measures for information protection are also described. The standards for methods and security procedure for the information in computer networks are enlisted. There is also an example of protecting one local data network (in this paper.

  18. DETOMIDINE AND BUTORPHANOL FOR STANDING SEDATION IN A RANGE OF ZOO-KEPT UNGULATE SPECIES.

    Science.gov (United States)

    Bouts, Tim; Dodds, Joanne; Berry, Karla; Arif, Abdi; Taylor, Polly; Routh, Andrew; Gasthuys, Frank

    2017-09-01

    General anesthesia poses risks for larger zoo species, like cardiorespiratory depression, myopathy, and hyperthermia. In ruminants, ruminal bloat and regurgitation of rumen contents with potential aspiration pneumonia are added risks. Thus, the use of sedation to perform minor procedures is justified in zoo animals. A combination of detomidine and butorphanol has been routinely used in domestic animals. This drug combination, administered by remote intramuscular injection, can also be applied for standing sedation in a range of zoo animals, allowing a number of minor procedures. The combination was successfully administered in five species of nondomesticated equids (Przewalski horse [ Equus ferus przewalskii; n = 1], onager [ Equus hemionus onager; n = 4], kiang [ Equus kiang ; n = 3], Grevy's zebra [ Equus grevyi ; n = 4], and Somali wild ass [ Equus africanus somaliensis; n = 7]), with a mean dose range of 0.10-0.17 mg/kg detomidine and 0.07-0.13 mg/kg butorphanol; the white ( Ceratotherium simum simum; n = 12) and greater one-horned rhinoceros ( Rhinoceros unicornis ; n = 4), with a mean dose of 0.015 mg/kg of both detomidine and butorphanol; and Asiatic elephant bulls ( Elephas maximus ; n = 2), with a mean dose of 0.018 mg/kg of both detomidine and butorphanol. In addition, the combination was successfully used for standing sedation in six species of artiodactylids: giraffe ( Giraffa camelopardalis reticulata; n = 3), western bongo ( Tragelaphus eurycerus eurycerus; n = 2), wisent ( Bison bonasus ; n = 5), yak ( Bos grunniens ; n = 1), water buffalo ( Bubalus bubalis ; n = 4) and Bactrian camel ( Camelus bactrianus ; n = 5). The mean dose range for artiodactylid species except bongo was 0.04-0.06 mg/kg detomidine and 0.03-0.06 mg/kg butorphanol. The dose in bongo, 0.15-0.20 mg/kg detomidine and 0.13-0.15 mg/kg butorphanol, was considerably higher. Times to first effect, approach, and recovery after antidote were short. The use of detomidine and butorphanol has

  19. Disentangling effects of growth and nutritional status on seabird stable isotope ratios

    Science.gov (United States)

    Sears, J.; Hatch, Shyla A.; O'Brien, D. M.

    2009-01-01

    A growing number of studies suggest that an individual's physiology affects its carbon and nitrogen stable isotope signatures, obscuring a signal often assumed to be only a reflection of diet and foraging location. We examined effects of growth and moderate food restriction on red blood cell (RBC) and feather ??15N and ??13C in rhinoceros auklet chicks (Cerorhinca monocerata), a piscivorous seabird. Chicks were reared in captivity and fed either control (75 g/day; n = 7) or ~40% restricted (40 g/day; n = 6) amounts of high quality forage fish. We quantified effects of growth on isotopic fractionation by comparing ??15N and ??13C in control chicks to those of captive, non-growing subadult auklets (n = 11) fed the same diet. To estimate natural levels of isotopic variation, we also collected blood from a random sample of free-living rhinoceros auklet adults and chicks in the Gulf of Alaska (n = 15 for each), as well as adult feather samples (n = 13). In the captive experiment, moderate food restriction caused significant depletion in ??15N of both RBCs and feathers in treatment chicks compared to control chicks. Growth also induced depletion in RBC ??15N, with chicks exhibiting lower ??15N when they were growing the fastest. As growth slowed, ??15N increased, resulting in an overall pattern of enrichment over the course of the nestling period. Combined effects of growth and restriction depleted ??15N in chick RBCs by 0.92???. We propose that increased nitrogen-use efficiency is responsible for 15N depletion in both growing and food-restricted chicks. ??15N values in RBCs of free-ranging auklets fell within a range of only 1.03???, while feather ??15N varied widely. Together, our captive and field results suggest that both growth and moderate food restriction can affect stable isotope ratios in an ecologically meaningful way in RBCs although not feathers due to greater natural variability in this tissue. ?? 2008 Springer-Verlag.

  20. The quandary of local people—Park relations in Nepal's Royal Chitwan National Park

    Science.gov (United States)

    Nepal, Sanjay K.; Weber, Karl E.

    1995-11-01

    This paper analyzes five major causes of park-people conflicts that have occurred in Nepal's Royal Chitwan National Park. The causes include illegal transactions of forest products from the park, livestock grazing in the park, illegal hunting and fishing, crop damage, and threats to human and animal life caused by wild animals from the park. The conflicts indicate a reciprocal relationship between the park and local people. They reflect the attitudes of local people and representatives of the park authority whose priorities and objectives largely diverge. The results show that people settled adjacent to the park are heavily dependent on its resources. Even in places where some, albeit few alternative sources exist, local people continue to trespass the park boundary as these sources are inadequate to ensure the fulfillment of local people's resource needs. Illegal transactions of resources continue throughout the year; however, they are less intense during summer due to flooding caused by the Rapti River, which forms the park boundary towards the northern section where this study is conducted. The frequency of local people's visits to the park is mainly determined by their age, distance between homesteads and park, and volume of crop loss caused by wild animals. Crop damage is the function of size of landholding, distance, and frequency of crop raid. Local people claim that they have no intention of letting their livestock graze in the park; however, the dense vegetation of the park attracts livestock grazing on riverbanks just outside the open park boundary. Many head of livestock are killed by carnivores of the park. Human casualties are mainly caused by sloth bear ( Melursus ursinus), tiger ( Panthera tigris), wild pig ( Sug scrofa), and rhinoceros ( Rhinoceros unicornis). There had been some earlier attempts to reconcile the conflicts by offering local people different kinds of compensations; however, these were unsuccessful measures. An integrated approach is

  1. In situ characterization of the black pigment from parietal art of the Rouffignac Cave with a portable XRF system

    International Nuclear Information System (INIS)

    Sanoit, J. de; Chambellan, D.; Plassard, F.

    2005-01-01

    The Rouffignac cave in Dordogne (France) is a palaeolithic site which contains, among others, an important patrimony of cave art realized with black pigment (mammoths, bisons, woolly rhinoceros, horses, ibexes). Although no direct dating has ever been realized to date, the graphic works of this cave are generally connected to the Magdalenian age by cross-dating (stylistic comparison). For the first time, in situ non destructive analyses of this pigment were able to be made due to the use of a portable XRF system (X-Ray Fluorescence). The experimental set-up is mainly constituted with a small X-ray generator (Bullet TM 40 kV, Moxtek), a SDD detector (silicon drift detector, Rontec 1102) cooled by a Peltier cryostat and an optical system, the whole being connected to a portable system for acquisition of spectrometric data. The experimental spectra obtained on the three rhinoceros frieze, the horse over the flint nodule, the ten mammoths frieze and some animals of the Grand Plafond showed a systematic presence of manganese in all figures. This hypothesis had been already suggested by Graziosi in 1956 in the review 'La Nature' after a destructive analysis of a sample taken on one of the horses of the Grand Plafond. A Monte-Carlo simulation allowed a first quantitative approach in the analysis of the black pigment of the drawings. We can assess that the used manganese ore does not only result from a unique source because variable ratios of manganese, barium and iron were probed in the black pigment. None of the studied drawing was executed exclusively with black charcoal that would have made the selection of a sample suitable for radiocarbon dating less complicated. Additional non destructive in situ analysis using a portable PIXE set-up (Particles Induced X-ray Emission) equipped with a 210 Po as radiation source can be foreseen to allow the detection of elements with atomic numbers (Z) between 11 and 16 in the first layers of the black pigment, there where the XRF

  2. Daylight Design of Office Buildings: Optimisation of External Solar Shadings by Using Combined Simulation Methods

    Directory of Open Access Journals (Sweden)

    Javier González

    2015-05-01

    Full Text Available Integrating daylight and energy performance with optimization into the design process has always been a challenge for designers. Most of the building environmental performance simulation tools require a considerable amount of time and iterations for achieving accurate results. Moreover the combination of daylight and energy performances has always been an issue, as different software packages are needed to perform detailed calculations. A simplified method to overcome both issues using recent advances in software integration is explored here. As a case study; the optimization of external shadings in a typical office space in Australia is presented. Results are compared against common solutions adopted as industry standard practices. Visual comfort and energy efficiency are analysed in an integrated approach. The DIVA (Design, Iterate, Validate and Adapt plug-in for Rhinoceros/Grasshopper software is used as the main tool, given its ability to effectively calculate daylight metrics (using the Radiance/Daysim engine and energy consumption (using the EnergyPlus engine. The optimization process is carried out parametrically controlling the shadings’ geometries. Genetic Algorithms (GA embedded in the evolutionary solver Galapagos are adopted in order to achieve close to optimum results by controlling iteration parameters. The optimized result, in comparison with conventional design techniques, reveals significant enhancement of comfort levels and energy efficiency. Benefits and drawbacks of the proposed strategy are then discussed.

  3. Plastic ingestion in marine-associated bird species from the eastern North Pacific.

    Science.gov (United States)

    Avery-Gomm, S; Provencher, J F; Morgan, K H; Bertram, D F

    2013-07-15

    In addition to monitoring trends in plastic pollution, multi-species surveys are needed to fully understand the pervasiveness of plastic ingestion. We examined the stomach contents of 20 bird species collected from the coastal waters of the eastern North Pacific, a region known to have high levels of plastic pollution. We observed no evidence of plastic ingestion in Rhinoceros Auklet, Marbled Murrelet, Ancient Murrelet or Pigeon Guillemot, and low levels in Common Murre (2.7% incidence rate). Small sample sizes limit our ability to draw conclusions about population level trends for the remaining fifteen species, though evidence of plastic ingestion was found in Glaucous-Winged Gull and Sooty Shearwater. Documenting levels of plastic ingestion in a wide array of species is necessary to gain a comprehensive understanding about the impacts of plastic pollution. We propose that those working with bird carcasses follow standard protocols to assess the levels of plastic ingestion whenever possible. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. African wildlife conservation and the evolution of hunting institutions

    Science.gov (United States)

    't Sas-Rolfes, Michael

    2017-11-01

    Hunting regulation presents a significant challenge for contemporary global conservation governance. Motivated by various incentives, hunters may act legally or illegally, for or against the interests of conservation. Hunter incentives are shaped by the interactions between unevenly evolving formal and informal institutions, embedded in socio-ecological systems. To work effectively for conservation, regulatory interventions must take these evolving institutional interactions into account. Drawing on analytical tools from evolutionary institutional economics, this article examines the trajectory of African hunting regulation and its consequences. Concepts of institutional dynamics, fit, scale, and interplay are applied to case studies of rhinoceros and lion hunting to highlight issues of significance to conservation outcomes. These include important links between different forms of hunting and dynamic interplay with institutions of trade. The case studies reveal that inappropriate formal regulatory approaches may be undermined by adaptive informal market responses. Poorly regulated hunting may lead to calls for stricter regulations or bans, but such legal restrictions may in turn perversely lead to more intensified and organised illegal hunting activity, further undermining conservation objectives. I conclude by offering insights and recommendations to guide more effective future regulatory interventions and priorities for further research. Specifically, I advocate approaches that move beyond simplistic regulatory interventions toward more complex, but supportive, institutional arrangements that align formal and informal institutions through inclusive stakeholder engagement.

  5. Climate warming and humans played different roles in triggering Late Quaternary extinctions in east and west Eurasia.

    Science.gov (United States)

    Wan, Xinru; Zhang, Zhibin

    2017-03-29

    Climate change and humans are proposed as the two key drivers of total extinction of many large mammals in the Late Pleistocene and Early Holocene, but disentangling their relative roles remains challenging owing to a lack of quantitative evaluation of human impact and climate-driven distribution changes on the extinctions of these large mammals in a continuous temporal-spatial dimension. Here, our analyses showed that temperature change had significant effects on mammoth (genus Mammuthus ), rhinoceros (Rhinocerotidae), horse (Equidae) and deer (Cervidae). Rapid global warming was the predominant factor driving the total extinction of mammoths and rhinos in frigid zones from the Late Pleistocene and Early Holocene. Humans showed significant, negative effects on extirpations of the four mammalian taxa, and were the predominant factor causing the extinction or major extirpations of rhinos and horses. Deer survived both rapid climate warming and extensive human impacts. Our study indicates that both the current rates of warming and range shifts of species are much faster than those from the Late Pleistocene to Holocene. Our results provide new insight into the extinction of Late Quaternary megafauna by demonstrating taxon-, period- and region-specific differences in extinction drivers of climate change and human disturbances, and some implications about the extinction risk of animals by recent and ongoing climate warming. © 2017 The Author(s).

  6. Digital surveillance: a novel approach to monitoring the illegal wildlife trade.

    Directory of Open Access Journals (Sweden)

    Amy L Sonricker Hansen

    Full Text Available A dearth of information obscures the true scale of the global illegal trade in wildlife. Herein, we introduce an automated web crawling surveillance system developed to monitor reports on illegally traded wildlife. A resource for enforcement officials as well as the general public, the freely available website, http://www.healthmap.org/wildlifetrade, provides a customizable visualization of worldwide reports on interceptions of illegally traded wildlife and wildlife products. From August 1, 2010 to July 31, 2011, publicly available English language illegal wildlife trade reports from official and unofficial sources were collected and categorized by location and species involved. During this interval, 858 illegal wildlife trade reports were collected from 89 countries. Countries with the highest number of reports included India (n = 146, 15.6%, the United States (n = 143, 15.3%, South Africa (n = 75, 8.0%, China (n = 41, 4.4%, and Vietnam (n = 37, 4.0%. Species reported as traded or poached included elephants (n = 107, 12.5%, rhinoceros (n = 103, 12.0%, tigers (n = 68, 7.9%, leopards (n = 54, 6.3%, and pangolins (n = 45, 5.2%. The use of unofficial data sources, such as online news sites and social networks, to collect information on international wildlife trade augments traditional approaches drawing on official reporting and presents a novel source of intelligence with which to monitor and collect news in support of enforcement against this threat to wildlife conservation worldwide.

  7. Distribution of stress on TMJ disc induced by use of chincup therapy: assessment by the finite element method

    Science.gov (United States)

    Calçada, Flávio Siqueira; Guimarães, Antônio Sérgio; Teixeira, Marcelo Lucchesi; Takamatsu, Flávio Atsushi

    2017-01-01

    ABSTRACT Objective: To assess the distribution of stress produced on TMJ disc by chincup therapy, by means of the finite element method. Methods: a simplified three-dimensional TMJ disc model was developed by using Rhinoceros 3D software, and exported to ANSYS software. A 4.9N load was applied on the inferior surface of the model at inclinations of 30, 40, and 50 degrees to the mandibular plane (GoMe). ANSYS was used to analyze stress distribution on the TMJ disc for the different angulations, by means of finite element method. Results: The results showed that the tensile and compressive stresses concentrations were higher on the inferior surface of the model. More presence of tensile stress was found in the middle-anterior region of the model and its location was not altered in the three directions of load application. There was more presence of compressive stress in the middle and mid-posterior regions, but when a 50o inclined load was applied, concentration in the middle region was prevalent. Tensile and compressive stresses intensities progressively diminished as the load was more vertically applied. Conclusions: stress induced by the chincup therapy is mainly located on the inferior surface of the model. Loads at greater angles to the mandibular plane produced distribution of stresses with lower intensity and a concentration of compressive stresses in the middle region. The simplified three-dimensional model proved useful for assessing the distribution of stresses on the TMJ disc induced by the chincup therapy. PMID:29160348

  8. Innovative 3D Model of the Human Middle Ear in High Resolution with a Histological Microgrinding Method: A Feasibility Study and Comparison with μCT

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    Susanne Bradel

    2017-01-01

    Full Text Available Conclusion. The development of a histological 3D model of the tympanic cavity visualizes the exact microanatomy of the sound conduction organ and is therefore essential for finite elements simulations and surgical training. Objectives. So far, no accurate histological 3D model of the sound conduction system existed in literature. For 3D reconstruction of the very fine structures inside and outside the auditory ossicles, a method based on histological slices allows a more differential analysis of both hard and soft tissues and could thus be superior to μCT. Method. A complete temporal bone was embedded in epoxy resin and microground in distances of about 34 μm. After photodocumentation of every plane, a 3D reconstruction was performed by using the Computer Aided Design (CAD program Rhinoceros 5®. For comparison, a μCT of the same specimen resulted in a 3D model of the calcified structures in the middle ear. Results. The histological 3D model gives an excellent overview to all anatomical soft and bony tissues of the human auditory ossicles. Specifically the fine blood vessel system and the exact dimension of cartilage areas inside the ossicles can be illustrated much more precisely than with μCT data. The present technique also allows the evaluation of the fine connecting ligaments inside the tympanic cavity.

  9. 3D modelling of microscopic structure of ring‑porous wood

    Directory of Open Access Journals (Sweden)

    R. Neugebauer

    2009-01-01

    Full Text Available Nowadays many scientific 3D models of wood are available. These models are suitable only for simulation of physical fields movements in wood but not for educational purposes especially demonstration of microscopic structure of wood.3D structure of ring-porous hardwood species was created. European ash (Faraxinus excelsior L. was selected due to its relatively simple wood structure than other wood species within ring-porous group. The model was created by manual modelling process in Rhinoceros 4.0 that is a software for visualization and modelling. Microslides were prepared from sample of ash to take micro photos that were used as a visual source for modelling. Beside these pictures made by authors of this paper other microphotos from wood anatomy publications were used. Created 3D model of ash is a VRML object (Virtual Reality Modelling Language. This format is available to viewing and presentation with severe freeware VRML browsers. These VRML browsers are available as a plug-in for standard internet browsers or as standalone VRML browsers. The model is useful for studying and teaching microscopic structure of ring‑porous hardwood species. It was created such a way to be as exact copy of original anatomical structure of ash wood as possible. The paper may be used as a cue for other authors to create their own 3D models of different wood kinds.

  10. A formal mammalian biostratigraphy for the Late Pleistocene of Britain

    Science.gov (United States)

    Currant, Andrew; Jacobi, Roger

    2001-10-01

    A series of distinctive mammalian assemblages spanning much of the British Late Pleistocene is defined on the basis of type localities and a formal biozonation proposed. The Joint Mitnor Cave mammal assemblage-zone includes the famous "Hippopotamus fauna" of the early part of the Last Interglacial complex (Oxygen Isotope Substage 5e). This is succeeded by the Bacon Hole mammal assemblage-zone in which hippopotamus is no longer present and species like mammoth, roe deer and northern vole re-enter the British region. This assemblage-zone appears to represent the later substages of OIS 5. A faunal grouping dominated by bison and reindeer is named the Banwell Bone Cave mammal assemblage-zone and is believed to correlate closely with the Early Devensian (OIS 4). The Pin Hole mammal assemblage-zone includes the familiar mammoth-steppe faunas of the Middle Devensian (OIS 3) dominated by horse, woolly rhinoceros and mammoth. The Lateglacial Interstadial is characterized by the Gough's Cave mammal assemblage-zone in which horse, red deer and humans are well represented (part of OIS 2). No definitive evidence for human activity can be found for a period spanning the Last Interglacial complex (OIS 5) and the Early Devensian (OIS 4). Human populations return to Britain with the Pin Hole mammal assemblage-zone fauna during the Middle Devensian (OIS 3) and reappear after the Dimlington Stadial during the Late Devensian (OIS 2) but in a different faunal association.

  11. Chemically mediated group formation in soil-dwelling larvae and pupae of the beetle Trypoxylus dichotomus

    Science.gov (United States)

    Kojima, Wataru; Ishikawa, Yukio; Takanashi, Takuma

    2014-09-01

    Many insects form groups through interactions among individuals, and these are often mediated by chemical, acoustic, or visual cues and signals. In spite of the diversity of soil-dwelling insects, their aggregation behaviour has not been examined as extensively as that of aboveground species. We investigated the aggregation mechanisms of larvae of the Japanese rhinoceros beetle Trypoxylus dichotomus, which live in groups in humus soil. In two-choice laboratory tests, 2nd- and 3rd-instar larvae gathered at conspecific larvae irrespective of the kinship. The ablation of maxillae, which bear chemosensilla, abolished aggregation behaviour. Intact larvae also exhibited aggregation behaviour towards a larval homogenate. These results suggest that larval aggregation is mediated by chemical cues. We also demonstrated that the mature larvae of T. dichotomus built their pupal cells close to a mesh bag containing a conspecific pupal cell, which indicated that larvae utilize chemical cues emanating from these cells to select the pupation site. Thus, the larvae of T. dichotomus may use chemical cues from the conspecifics in two different contexts, i.e. larval aggregation and pupation site selection. Using conspecific cues, larvae may be able to choose suitable locations for foraging or building pupal cells. The results of the present study highlight the importance of chemical information in belowground ecology.

  12. Mechanisms regulating nutrition-dependent developmental plasticity through organ-specific effects in insects

    Science.gov (United States)

    Koyama, Takashi; Mendes, Cláudia C.; Mirth, Christen K.

    2013-01-01

    Nutrition, via the insulin/insulin-like growth factor (IIS)/Target of Rapamycin (TOR) signaling pathway, can provide a strong molding force for determining animal size and shape. For instance, nutrition induces a disproportionate increase in the size of male horns in dung and rhinoceros beetles, or mandibles in staghorn or horned flour beetles, relative to body size. In these species, well-fed male larvae produce adults with greatly enlarged horns or mandibles, whereas males that are starved or poorly fed as larvae bear much more modest appendages. Changes in IIS/TOR signaling plays a key role in appendage development by regulating growth in the horn and mandible primordia. In contrast, changes in the IIS/TOR pathway produce minimal effects on the size of other adult structures, such as the male genitalia in fruit flies and dung beetles. The horn, mandible and genitalia illustrate that although all tissues are exposed to the same hormonal environment within the larval body, the extent to which insulin can induce growth is organ specific. In addition, the IIS/TOR pathway affects body size and shape by controlling production of metamorphic hormones important for regulating developmental timing, like the steroid molting hormone ecdysone and sesquiterpenoid hormone juvenile hormone. In this review, we discuss recent results from Drosophila and other insects that highlight mechanisms allowing tissues to differ in their sensitivity to IIS/TOR and the potential consequences of these differences on body size and shape. PMID:24133450

  13. PARAMETRIC MODELING, CREATIVITY, AND DESIGN: TWO EXPERIENCES WITH ARCHITECTURE’ STUDENTS

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    Wilson Florio

    2012-02-01

    Full Text Available The aim of this article is to reflect on the use of the parametric modeling in two didactic experiences. The first experiment involved resources of the Paracloud program and its relation with the Rhinoceros program, that resulted in the production of physical models produced with the aid of the laser cutting. In the second experiment, the students had produced algorithms in the Grasshopper, resulting in families of structures and coverings. The study objects are both the physical models and digital algorithms resultants from this experimentation. For the analysis and synthesis of the results, we adopted four important assumptions: 1. the value of attitudes and environment of work; 2. the importance of experimentation and improvisation; 3. understanding of the design process as a situated act and as a ill-defined problem; 4. the inclusion of creative and critical thought in the disciplines. The results allow us to affirm that the parametric modeling stimulates creativity, therefore allowing combination of different parameters, that result in unexpected discoveries. Keywords: Teach-Learning, Parametric Modeling, Laser Cutter, Grasshopper, Design Process, Creativity.

  14. Three-dimensional finite element analysis of glass fiber and cast metal posts with different alloys for reconstruction of teeth without ferrule.

    Science.gov (United States)

    Verri, Fellippo Ramos; Okumura, Marlice Hayumi Theles; Lemos, Cleidiel Aparecido Araujo; Almeida, Daniel Augusto de Faria; de Souza Batista, Victor Eduardo; Cruz, Ronaldo Silva; Oliveira, Hiskell Francine Fernandes; Pellizzer, Eduardo Piza

    2017-11-01

    The aim of this study was to evaluate different materials for restoration of teeth without ferrule by three-dimensional (3D) finite element analysis (FEA). Five models simulating the maxillary central incisor and surrounding bone were simulated according to the type of post: glass fibre post (GFP) or cast metal post (CMP) with different alloys such as gold (Au), silver-palladium (AgPd), copper-aluminum (CuAl) and nickel-chromium (NiCr). Models were designed using Invesalius and Rhinoceros. FEAs were made using FEMAP and NeiNastran, with an applied axial force of 100 N and oblique occlusal load at 45°. Stress distribution among groups was analysed by two-way analysis of variance (ANOVA), followed by post-hoc Tukey's test. The GFP showed the best stress distribution in the post, followed by CMP with Au, AgPd, CuAl and NiCr alloys, respectively (p  .05). Under oblique load, the GFP generated the highest values of tension among the models, followed by the CMP with NiCr alloy than other models (p < .001). The use of GFP resulted in a lower stress concentration in the post, but increased stress in the tooth without ferrule. The CMP with NiCr alloy exhibited the highest stress distribution among other CMP. To avoid higher stress in teeth, alloys of Au, AgPd and CuAl, respectively, are recommended.

  15. A rhinocerotid-dominated megafauna at the MIS6-5 transition: The late Middle Pleistocene Coc Muoi assemblage, Lang Son province, Vietnam

    Science.gov (United States)

    Bacon, Anne-Marie; Antoine, Pierre-Olivier; Huong, Nguyen Thi Mai; Westaway, Kira; Tuan, Nguyen Anh; Duringer, Philippe; Zhao, Jian-xin; Ponche, Jean-Luc; Dung, Sam Canh; Nghia, Truong Huu; Minh, Tran Thi; Son, Pham Thanh; Boyon, Marc; Thuy, Nguyen Thi Kim; Blin, Amandine; Demeter, Fabrice

    2018-04-01

    Little is known about the ecosystems in the north of the Indochinese peninsula at the Middle-Late Pleistocene transition. In this paper, we analyzed the new fauna from Coc Muoi cave, Lang Son province, northeast Vietnam. In comparison with other well-documented faunas from the region, that of Coc Muoi is distinguished by the predominance of rhinoceroses among diverse large-bodied herbivores. The results of the OSL and pIR-IRSL dating of the cave sediments and U-series dating of flowstones indicate a potential age range of 148-117 ka for the fauna (MIS6-5). The analysis of age-at-death distributions of rhinoceroses, wild cattle, sambar deer, and wild pig, does not show any apparent selectivity of age classes. We also focused our study on rhinoceroses, tapirs, and wild cattle by analyzing the prevalence of hypoplastic defects on deciduous and permanent teeth, in an attempt to assess the health status of the taxa during their first years of growth. The health status of large-bodied herbivores (kouprey and rhinoceros) reveals the importance of stressors (biotic and abiotic) in the rainforest environment during a period of marked climatic transition (MIS6-5) in comparison with other MIS5-4 well-documented faunas from the region.

  16. Possibilities of Preoperative Medical Models Made by 3D Printing or Additive Manufacturing.

    Science.gov (United States)

    Salmi, Mika

    2016-01-01

    Most of the 3D printing applications of preoperative models have been focused on dental and craniomaxillofacial area. The purpose of this paper is to demonstrate the possibilities in other application areas and give examples of the current possibilities. The approach was to communicate with the surgeons with different fields about their needs related preoperative models and try to produce preoperative models that satisfy those needs. Ten different kinds of examples of possibilities were selected to be shown in this paper and aspects related imaging, 3D model reconstruction, 3D modeling, and 3D printing were presented. Examples were heart, ankle, backbone, knee, and pelvis with different processes and materials. Software types required were Osirix, 3Data Expert, and Rhinoceros. Different 3D printing processes were binder jetting and material extrusion. This paper presents a wide range of possibilities related to 3D printing of preoperative models. Surgeons should be aware of the new possibilities and in most cases help from mechanical engineering side is needed.

  17. The influence of trophic level and feeding location of the levels of organochlorine contaminants in seabird eggs as revealed by stable carbon and nitrogen isotope analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hobson, K. [Canadian Wildlife Service, Saskatoon, Saskatchewan (Canada); Jarman, W.M.; Bott, J.A.; Bacon, C.E. [Univ. of California, Santa Cruz (Canada). Inst. of Marine Sciences; Sydeman, W. [Point Reyes Bird Observatory, Stinson Beach, CA (United States)

    1994-12-31

    Seabird eggs have been used extensively to assay contaminants in marine food webs, but links to trophic level or feeding location have remained poorly understood due to limitations inherent in conventional dietary studies. Stable-isotope analysis of bird eggs may be used to infer trophic position and feeding location of adult seabirds and can be readily correlated with measurements of egg contaminant levels. The authors measured stable-carbon ({delta}{sup 13}C) and nitrogen ({delta}{sup 15}N) isotope abundance, and organochlorine contaminants (DDTs, PCBs, chlordanes, etc.) in eggs from Cassin`s Auklet (Ptychoramphus aleutica), Common Murre (Uria aalge), Pigeon Guillemot (Cepphus columba). Rhinoceros Auklet (Cerorhinca monocerata), Pelagic Cormorant (Phalacrocorax pelagicus), Brandt`s Cormorant (Phalacrocorax penicillatus), and Western Gull (Larus) from Southeast Farallon Island together with rockfish (Sebastes spp.), anchovy (Engraulis spp.), and euphausiid prey from the Gulf of the Farallones. Consistent with its planktivorous diet and pelagic feeding habits, Cassin`s Auklet showed the lowest mean {delta}{sup 15}N value and the least enriched {delta}{sup 13}C values. Measures of trophic level and foraging location were constructed for all other seabirds relative to these isotopic endpoints. Contaminant levels in the eggs and fish will be interpreted in light of the stable-isotope results.

  18. FEM analysis of different dental root canal-post systems in young permanent teeth.

    Science.gov (United States)

    Vitale, M C; Chiesa, M; Coltellaro, F; Bignardi, C; Celozzi, M; Poggio, C

    2008-09-01

    Aim of this work was to carry out a comparative evaluation of the structural behaviour of different root canal posts (cylindrical, conical and triple conical) fitted in a second lower bicuspid and subjected to compression and bending test. This study has been carried out by numerical method of structural analysis of finite elements (FEM, Finite Element Method). Different tridimensional models were obtained by CAT images of an extracted tooth, endodontically treated, filled with guttapercha and triple conical glass post. Images have been elaborated by a software for images (Mimics and Ansys) and CAD (Rhinoceros 3 D). In the models a II Class restoration has been virtually created. In the numerical simulation dental tissues (enamel, dentine and root cement), guttapercha, root canal cement, different posts, different techniques of cementation and crown restoration (composites and adhesive systems) have been considered. Strain distributions in dental tissues, in root canal cement and in posts have been compared. The equivalent tensions and the single components (traction, compression and cut) have been analysed. In all examined posts, the most strained part is resulted the coronal one, even if the total tension, in the different tooth-post analyzed systems, resulted uniformly distributed. A similar behaviour was shown by the root canal cement. According to the analyzed conditions of bond and load, varying according to the geometry of the considered posts, our results confirm that there is no substantial difference of deformation in posts, root canal cement and treated tooth.

  19. Optimization of Ducted Propeller Design for the ROV (Remotely Operated Vehicle using CFD

    Directory of Open Access Journals (Sweden)

    Aldias Bahatmaka

    2016-11-01

    Full Text Available The development of underwater robot technology is growing rapidly. For reaching the best performance, it is important that the innovation on ROV should be focused on the thruster and propeller.In this research, the ducted propeller thruster is used while three types of SHUSKHIN nozzle are selected. The design is compared in accordance with the thruster that has been made as the propulsion device of underwater robots. Each type of the thruster model indicates different force and torque. For the analysis, each model is built in Computer Aided Design (Rhinoceros program packages and Computational Fluid Dynamics (CFD to find the most optimal model which can produce the highest thrust. Among the entire model, the Kaplan series (Ka5-75 with the type C of nozzle has the highest thrust which is 2.53 N or 25.24% of extra thrust.For the optimization of thrust, genetic Algorithms (GA is used. The GA can search for parameters in large multi-dimensional design space. Thus, the principle can be applied for determining the initial propeller that produces optimum thrust of ROV. The GA has successfully shown able to obtain an optimal set parameters for propeller characteristics with the best performance.

  20. Flow structures around a beetle in a tethered flight

    Science.gov (United States)

    Lee, Boogeon; Oh, Sehyeong; Park, Hyungmin; Choi, Haecheon

    2017-11-01

    In the present study, through a wind-tunnel experiment, we visualize the flow in a tethered flight of a rhinoceros beetle using a smoke-wire visualization technique. Measurements are done at five side planes along the wind span while varying the body angle (angle between the horizontal and the body axis) to investigate the influence of the stroke plane angle that was observed to change depending on the flight mode such as hovering, forward and takeoff flights so on. Observing that a large attached leading-edge vortex is only found on the hindwing, it is inferred that most of the aerodynamic forces would be generated by hindwings (flexible inner wings) compared to the elytra (hard outer wings). In addition, it is observed to use unsteady lift-generating mechanisms such as clap-and-fling, wing-wing interaction and wake capture. Finally, we discuss the relation between the advance ratio and Strouhal number by adjusting free-stream velocity and the body angle (i.e., angle of wake-induced flow). Supported by a Grant to Bio-Mimetic Robot Research Center Funded by Defense Acquisition Program Administration, and by ADD, Korea (UD130070ID).

  1. Comprehensive Serology Based on a Peptide ELISA to Assess the Prevalence of Closely Related Equine Herpesviruses in Zoo and Wild Animals.

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    Azza Abdelgawad

    Full Text Available Equine herpesvirus type 1 (EHV-1 causes respiratory disorders and abortion in equids while EHV-1 regularly causes equine herpesvirus myeloencephalopathy (EHM, a stroke-like syndrome following endothelial cell infection in horses. Both EHV-1 and EHV-9 infections of non-definitive hosts often result in neuronal infection and high case fatality rates. Hence, EHV-1 and EHV-9 are somewhat unusual herpesviruses and lack strict host specificity, and the true extent of their host ranges have remained unclear. In order to determine the seroprevalence of EHV-1 and EHV-9, a sensitive and specific peptide-based ELISA was developed and applied to 428 sera from captive and wild animals representing 30 species in 12 families and five orders. Members of the Equidae, Rhinocerotidae and Bovidae were serologically positive for EHV-1 and EHV-9. The prevalence of EHV-1 in the sampled wild zebra populations was significantly higher than in zoos suggesting captivity may reduce exposure to EHV-1. Furthermore, the seroprevalence for EHV-1 was significantly higher than for EHV-9 in zebras. In contrast, EHV-9 antibody prevalence was high in captive and wild African rhinoceros species suggesting that they may serve as a reservoir or natural host for EHV-9. Thus, EHV-1 and EHV-9 have a broad host range favoring African herbivores and may have acquired novel natural hosts in ecosystems where wild equids are common and are in close contact with other perissodactyls.

  2. Comprehensive Serology Based on a Peptide ELISA to Assess the Prevalence of Closely Related Equine Herpesviruses in Zoo and Wild Animals.

    Science.gov (United States)

    Abdelgawad, Azza; Hermes, Robert; Damiani, Armando; Lamglait, Benjamin; Czirják, Gábor Á; East, Marion; Aschenborn, Ortwin; Wenker, Christian; Kasem, Samy; Osterrieder, Nikolaus; Greenwood, Alex D

    2015-01-01

    Equine herpesvirus type 1 (EHV-1) causes respiratory disorders and abortion in equids while EHV-1 regularly causes equine herpesvirus myeloencephalopathy (EHM), a stroke-like syndrome following endothelial cell infection in horses. Both EHV-1 and EHV-9 infections of non-definitive hosts often result in neuronal infection and high case fatality rates. Hence, EHV-1 and EHV-9 are somewhat unusual herpesviruses and lack strict host specificity, and the true extent of their host ranges have remained unclear. In order to determine the seroprevalence of EHV-1 and EHV-9, a sensitive and specific peptide-based ELISA was developed and applied to 428 sera from captive and wild animals representing 30 species in 12 families and five orders. Members of the Equidae, Rhinocerotidae and Bovidae were serologically positive for EHV-1 and EHV-9. The prevalence of EHV-1 in the sampled wild zebra populations was significantly higher than in zoos suggesting captivity may reduce exposure to EHV-1. Furthermore, the seroprevalence for EHV-1 was significantly higher than for EHV-9 in zebras. In contrast, EHV-9 antibody prevalence was high in captive and wild African rhinoceros species suggesting that they may serve as a reservoir or natural host for EHV-9. Thus, EHV-1 and EHV-9 have a broad host range favoring African herbivores and may have acquired novel natural hosts in ecosystems where wild equids are common and are in close contact with other perissodactyls.

  3. Eco-epidemiological and pathological features of wildlife mortality events related to cyanobacterial bio-intoxication in the Kruger National Park, South Africa

    Directory of Open Access Journals (Sweden)

    Roy Bengis

    2016-10-01

    Full Text Available Over the past decade, several clustered, multispecies, wildlife mortality events occurred in the vicinity of two man-made earthen dams in the southern and south central regions of the Kruger National Park, South Africa. On field investigation, heavy cyanobacterial blooms were visible in these impoundments and analysis of water samples showed the dominance of Microcystis spp. (probably Microcystis aeruginosa. Macroscopic lesions seen at necropsy and histopathological lesions were compatible with a diagnosis of cyanobacterial intoxication. Laboratory toxicity tests and assays also confirmed the presence of significant levels of microcystins in water from the two dams. These outbreaks occurred during the dry autumn and early winter seasons when water levels in these dams were dropping, and a common feature was that all the affected dams were supporting a large number of hippopotamuses (Hippopotamus amphibius. It is hypothesised that hippopotamus’ urine and faeces, together with agitation of the sediments, significantly contributed to internal loading of phosphates and nitrogen – leading to eutrophication of the water in these impoundments and subsequent cyanobacterial blooms. A major cause for concern was that a number of white rhinoceros (Ceratotherium simum were amongst the victims of these bio-intoxication events. This publication discusses the eco-epidemiology and pathology of these clustered mortalities, as well as the management options considered and eventually used to address the problem.

  4. The taphonomy of a Middle Devensian (MIS 3) vertebrate assemblage from Lynford, Norfolk, UK, and its implications for Middle Palaeolithic subsistence strategies

    Science.gov (United States)

    Schreve, Danielle C.

    2006-07-01

    The association of a rich lithic assemblage with a Middle Devensian mammalian assemblage at Lynford was initially thought indicative of a mammoth butchery locality, a rare occurrence for a European Middle Palaeolithic open site. However, taphonomic analyses suggest that the specimens have very different depositional histories and were incorporated into a palaeochannel in several stages. Most specimens are extremely fragmentary, probably the result of extensive trampling, and signs of weathering and root-damage provide further indications of exposure before burial. Carnivore damage is minimal but establishing the degree of interaction between the mammal fauna and alternative predators, such as Neanderthals, is problematic. Direct evidence of butchery is not present and the best indication of any form of mammoth exploitation lies in more circumstantial evidence such as the virtual absence of long bones from the main channel deposit and the mammoth age profiles. Instances of pathologies are also unusually common in the mammoths, implying that their greater vulnerability may have led to an accelerated demise either naturally or at the hands of a predator. The best evidence for direct faunal exploitation at the site is from green bone fractures and broken teeth that suggest marrow extraction in horse, reindeer and woolly rhinoceros. Copyright

  5. Herds overhead: Nimbadon lavarackorum (Diprotodontidae, heavyweight marsupial herbivores in the Miocene forests of Australia.

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    Karen H Black

    Full Text Available The marsupial family Diprotodontidae (Diprotodontia, Vombatiformes is a group of extinct large-bodied (60-2500 kg wombat-like herbivores that were common and geographically widespread in Cenozoic fossil deposits of Australia and New Guinea. Typically they are regarded to be gregarious, terrestrial quadrupeds and have been likened in body form among placental groups to sheep, rhinoceros and hippopotami. Arguably, one of the best represented species is the zygomaturine diprotodontid Nimbadon lavarackorum which is known from exceptionally well-preserved cranial and postcranial material from the middle Miocene cave deposit AL90, in the Riversleigh World Heritage Area, northwestern Queensland. Here we describe and functionally analyse the appendicular skeleton of Nimbadon lavarackorum and reveal a far more unique lifestyle for this plesiomorphic and smallest of diprotodontids. Striking similarities are evident between the skeleton of Nimbadon and that of the extant arboreal koala Phascolarctos cinereus, including the powerfully built forelimbs, highly mobile shoulder and elbow joints, proportionately large manus and pes (both with a semi-opposable digit I and exceedingly large, recurved and laterally compressed claws. Combined with the unique (among australidelphians proportionately shortened hindlimbs of Nimbadon, these features suggest adept climbing ability, probable suspensory behaviour, and an arboreal lifestyle. At approximately 70 kg, Nimbadon is the largest herbivorous mammal to have occupied the forest canopies of Australia - an ecological niche that is no longer occupied in any Australian ecosystem and one that further expands the already significant niche diversity displayed by marsupials during the Cenozoic.

  6. Herds Overhead: Nimbadon lavarackorum (Diprotodontidae), Heavyweight Marsupial Herbivores in the Miocene Forests of Australia

    Science.gov (United States)

    Black, Karen H.; Camens, Aaron B.; Archer, Michael; Hand, Suzanne J.

    2012-01-01

    The marsupial family Diprotodontidae (Diprotodontia, Vombatiformes) is a group of extinct large-bodied (60–2500 kg) wombat-like herbivores that were common and geographically widespread in Cenozoic fossil deposits of Australia and New Guinea. Typically they are regarded to be gregarious, terrestrial quadrupeds and have been likened in body form among placental groups to sheep, rhinoceros and hippopotami. Arguably, one of the best represented species is the zygomaturine diprotodontid Nimbadon lavarackorum which is known from exceptionally well-preserved cranial and postcranial material from the middle Miocene cave deposit AL90, in the Riversleigh World Heritage Area, northwestern Queensland. Here we describe and functionally analyse the appendicular skeleton of Nimbadon lavarackorum and reveal a far more unique lifestyle for this plesiomorphic and smallest of diprotodontids. Striking similarities are evident between the skeleton of Nimbadon and that of the extant arboreal koala Phascolarctos cinereus, including the powerfully built forelimbs, highly mobile shoulder and elbow joints, proportionately large manus and pes (both with a semi-opposable digit I) and exceedingly large, recurved and laterally compressed claws. Combined with the unique (among australidelphians) proportionately shortened hindlimbs of Nimbadon, these features suggest adept climbing ability, probable suspensory behaviour, and an arboreal lifestyle. At approximately 70 kg, Nimbadon is the largest herbivorous mammal to have occupied the forest canopies of Australia - an ecological niche that is no longer occupied in any Australian ecosystem and one that further expands the already significant niche diversity displayed by marsupials during the Cenozoic. PMID:23185250

  7. Zebra Alphaherpesviruses (EHV-1 and EHV-9: Genetic Diversity, Latency and Co-Infections

    Directory of Open Access Journals (Sweden)

    Azza Abdelgawad

    2016-09-01

    Full Text Available Alphaherpesviruses are highly prevalent in equine populations and co-infections with more than one of these viruses’ strains frequently diagnosed. Lytic replication and latency with subsequent reactivation, along with new episodes of disease, can be influenced by genetic diversity generated by spontaneous mutation and recombination. Latency enhances virus survival by providing an epidemiological strategy for long-term maintenance of divergent strains in animal populations. The alphaherpesviruses equine herpesvirus 1 (EHV-1 and 9 (EHV-9 have recently been shown to cross species barriers, including a recombinant EHV-1 observed in fatal infections of a polar bear and Asian rhinoceros. Little is known about the latency and genetic diversity of EHV-1 and EHV-9, especially among zoo and wild equids. Here, we report evidence of limited genetic diversity in EHV-9 in zebras, whereas there is substantial genetic variability in EHV-1. We demonstrate that zebras can be lytically and latently infected with both viruses concurrently. Such a co-occurrence of infection in zebras suggests that even relatively slow-evolving viruses such as equine herpesviruses have the potential to diversify rapidly by recombination. This has potential consequences for the diagnosis of these viruses and their management in wild and captive equid populations.

  8. Utilizing new Mammal faunas for calibration of paleomagnetostratigraphy in the Kochkor basin, Kyrgyzstan.

    Science.gov (United States)

    McLaughlin, W. N. F.; Weldon, R. J.; Abdrakhmatov, K.; Hopkins, S. S. B.

    2015-12-01

    Kyrgyzstan is the most seismically active nation in the world, owning to the uplift of the Tien Shan mountain range. This record of faulting is far from simple though, with different dates of initiation spanning tens of millions of years in the published literature. Differentiating between these extremes is vital in terms of constructing earthquake hazard maps for the country. Much of the high degree of uncertainty comes from a lack of datable rocks within the time frame associated with the faulting. While paleomagnetic stratigraphic analyses provide highly accurate ages, they in turn need calibration points to preclude multiple possible matches to a global geochronological framework. Herein I provide the first description of Neogene fossil mammal faunas to act as biostratigraphic control for the paleomagnetic data. Dominating the faunal assemblage is rhinoceros species, Chilotherium anderssoni. This taxon is globally only known from 8.7-5.3 million years ago, constraining much of the stratigraphy to the latest Miocene to Pliocene. Additional taxa include Gazella, Samotherium, Hipparion, Hyaenaictithirium, Paleolagus, and Pliocervidae. As fossils are limited to the Miocene/Pliocene Chu Formation, we have also collected additional paleomagnetic samples from the lower Shamsi Formation to extend the age range covered and constraint the initiation of faulting.

  9. Pre-Rationalized Parametric Designing of Roof Shells Formed by Repetitive Modules of Catalan Surfaces

    Directory of Open Access Journals (Sweden)

    Jolanta Dzwierzynska

    2018-04-01

    Full Text Available The aim of the study is to develop an original, methodical, and practical approach to the early stages of parametric design of roof shells formed by repetitive modules of Catalan surfaces. It is presented on the example of designing the roof shells compound of four concrete elements. The designing process proposed by us consists in linking geometric shaping of roofs’ models with their structural analysis and optimization. Contrary to other methods, which use optimization process in order to find free roof forms, we apply it in order to explore and improve design alternatives. It is realized with the application of designing tools working in Rhinoceros 3D software. The flexible scripts elaborated by us, in order to achieve roofs’ models of regular and symmetrical shapes, are converted into simulation models to perform structural analysis. It is mainly focused on how the roof shells perform dependently on their geometric characteristics. The simulation enables one to evaluate various roof shells’ shapes, as well as to select an optimal design solution. The proposed approach to the conceptual design process may drive the designing to achieve geometric and structural forms which not only follow the design intentions but also target better results.

  10. Eco-epidemiological and pathological features of wildlife mortality events related to cyanobacterial bio-intoxication in the Kruger National Park, South Africa

    Directory of Open Access Journals (Sweden)

    Roy Bengis

    2016-02-01

    Full Text Available Over the past decade, several clustered, multispecies, wildlife mortality events occurred in the vicinity of two man-made earthen dams in the southern and south central regions of the Kruger National Park, South Africa. On field investigation, heavy cyanobacterial blooms were visible in these impoundments and analysis of water samples showed the dominance of Microcystis spp. (probably Microcystis aeruginosa. Macroscopic lesions seen at necropsy and histopathological lesions were compatible with a diagnosis of cyanobacterial intoxication. Laboratory toxicity tests and assays also confirmed the presence of significant levels of microcystins in water from the two dams. These outbreaks occurred during the dry autumn and early winter seasons when water levels in these dams were dropping, and a common feature was that all the affected dams were supporting a large number of hippopotamuses (Hippopotamus amphibius. It is hypothesised that hippopotamus’ urine and faeces, together with agitation of the sediments, significantly contributed to internal loading of phosphates and nitrogen – leading to eutrophication of the water in these impoundments and subsequent cyanobacterial blooms. A major cause for concern was that a number of white rhinoceros (Ceratotherium simum were amongst the victims of these bio-intoxication events. This publication discusses the eco-epidemiology and pathology of these clustered mortalities, as well as the management options considered and eventually used to address the problem.

  11. NEW DATA ON THE LOWER PLEISTOCENE (GELASIAN LIGNITE BEDS OF CASTEL SAN PIETRO (RIETI, CENTRAL ITALY

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    LUCA PANDOLFI

    2017-04-01

    Full Text Available A multiproxy study of the lignite-bearing lacustrine sequence cropping out at Castel San Pietro (CSP (Rieti, central Italy provides new insights on the paleoenvironmental evolution of an extensional basin, located on the Tyrrhenian side of the Apennine range. The CSP fossiliferous levels reflect deposition in a stable lacustrine environment. Mammal remains collected from this locality during the past centuries include Stephanorhinus etruscus, Anancus arvernensis and the beaver Castor fiber. The co-existence of the Etruscan rhinoceros and the mastodont suggests a middle Villafranchian age (Montopoli or Coste San Giacomo Faunal Unit for the lignite beds of CSP. New paleoflora findings are in agreement with an attribution to the Piacenzian or Gelasian age for these deposits whilst the presence of the ostracod Qinghaicypris cf. Q. riojensis suggests a time interval from Zanclean to Gelasian (between ~4.5 and ~1.8 Ma. Accordingly, the lignite beds of CSP most likely were deposited during the Gelasian (from ca. 2.5 to 1.8 Ma; from Montopoli to Coste San Giacomo FUs or possibly to Olivola FU in forested area and warm and humid conditions.

  12. Forensic Science in Support of Wildlife Conservation Efforts - Developments in Genetic Approaches in Taiwan.

    Science.gov (United States)

    Hsieh, H M; Tsai, L C; Lee, J Ci

    2011-01-01

    To control illegal wildlife-product trade and protect endangered species of animals, unambiguous identification of the animal specimens is vitally important. Genetic approaches were adopted to identify animal species for conservation and to prevent their fraudulent misidentification in Taiwan, especially for samples of animal residues, powders, and processed products. PCR or nested PCR based on the nature of DNA was used for amplification of cyt b, COI, CHD, and D-loop DNA fragments. Sequences of these fragments were compared with those registered in DNA databases and phylogenetic analysis was performed. The established methods were applied in forensic cases for support of conservation efforts and they were proved to be robust. For conservation animal identification, various samples seized by law enforcement agents have been identified by our systems as rhinoceros horns, Indian sawback turtles, shahtoosh, ivories, dolphins, whales, etc. The systems were also successfully used in investigating the illegal trade of commercial turtle shells and the fraudulent misidentification of food contents on product labels in Taiwanese markets. This review summarizes the work conducted in our laboratory and describes the Taiwan experience. Copyright © 2011 Central Police University.

  13. Effect of different livestock dungs as inoculum on food waste anaerobic digestion and its kinetics.

    Science.gov (United States)

    Dhamodharan, Kondusamy; Kumar, Vikas; Kalamdhad, Ajay S

    2015-03-01

    The aim of this study was to evaluate the effect of different livestock inoculums on the anaerobic digestion of food waste (FW). Five different livestock dungs i.e., poultry dung (PD), goat dung (GD), cow dung (CD), piggery dung (PGD) and rhinoceros dung (RD) were utilized as inoculums and their effects were valued in various food to microorganism (F/M) ratios in batch reactors. Different livestock dungs achieved higher methane production and volatile solids (VS) reduction in different F/M ratios such as PD, GD, CD, PGD and RD achieved at F/M ratio maintained at 1.5, 2, 2, 1.5 and 1.5, respectively. The results indicated that CD and PGD inoculum were more suitable for the anaerobic digestion of FW than other livestock dungs. Reactors inoculated with CD achieved higher methane production (227mLg(-1)VS degraded) and volatile solids degradation (54.58%) at F/M ratio maintained at 2. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Ciliate protozoa

    International Nuclear Information System (INIS)

    Dehority, B.A.

    2005-01-01

    Gruby and Delafond discovered the rumen protozoa in 1843 and suggested that their digestive activity was the primary means by which ruminants could survive on an all-plant diet. However, subsequent studies have clearly shown that bacteria actually play a prominent role in the fermentation of plant materials, and fungi are also involved. The rumen ciliates range in size from 18 to 500 μm and can be enumerated and identified microscopically at relatively low magnifications. On the basis of cell morphology, they have been classified into at least five families containing 24 different genera. Although new species are still being reported yearly, the last summary was compiled in 1992 by Williams and Coleman and listed 257 separate species. Ciliate protozoa belonging to different families are found in most other herbivorous mammals. This includes those animals in which fermentation occurs in the hindgut, such as the horse, elephant, rhinoceros and capybara, as well as in the non-ruminant foregut fomenters, i.e. camelids, hippopotamus and kangaroo. These ciliates can be enumerated and identified using the methods and techniques presented in this chapter, presuming appropriate procedures are used for obtaining representative samples from the animal

  15. RotCFD Analysis of the AH-56 Cheyenne Hub Drag

    Science.gov (United States)

    Solis, Eduardo; Bass, Tal A.; Keith, Matthew D.; Oppenheim, Rebecca T.; Runyon, Bryan T.; Veras-Alba, Belen

    2016-01-01

    In 2016, the U.S. Army Aviation Development Directorate (ADD) conducted tests in the U.S. Army 7- by 10- Foot Wind Tunnel at NASA Ames Research Center of a nonrotating 2/5th-scale AH-56 rotor hub. The objective of the tests was to determine how removing the mechanical control gyro affected the drag. Data for the lift, drag, and pitching moment were recorded for the 4-bladed rotor hub in various hardware configurations, azimuth angles, and angles of attack. Numerical simulations of a selection of the configurations and orientations were then performed, and the results were compared with the test data. To generate the simulation results, the hardware configurations were modeled using Creo and Rhinoceros 5, three-dimensional surface modeling computer-aided design (CAD) programs. The CAD model was imported into Rotorcraft Computational Fluid Dynamics (RotCFD), a computational fluid dynamics (CFD) tool used for analyzing rotor flow fields. RotCFD simulation results were compared with the experimental results of three hardware configurations at two azimuth angles, two angles of attack, and with and without wind tunnel walls. The results help validate RotCFD as a tool for analyzing low-drag rotor hub designs for advanced high-speed rotorcraft concepts. Future work will involve simulating additional hub geometries to reduce drag or tailor to other desired performance levels.

  16. A conservation model for black rhino

    Directory of Open Access Journals (Sweden)

    H.C. Hearne

    2003-12-01

    Full Text Available Over the past thirty years the black rhinoceros (Diceros bicornis population in Africa has declined from about 65 000 to 3 500. In contrast the South African and Namibian population has increased four-fold to 1 000 over the same period. The recently developed national conservation strategy for black rhino has as its main goal a further four-fold increase in the current population in as short a period as possible. To achieve this, the growth rate of the population as a whole will have to be maximised. This involves removing animals from areas where the population is approaching the ecological carrying capacity and establishing new viable populations in other suitable reserves. A model incorporating what is known about the population biology of black rhino, was developed to give guidance to managers on the most appropirate harvesting strategy to adopt for their populations; in particular, to determine the rate of removals and the age and sex of individuals to be removed to attain the conservation goal as soon as possible.

  17. Nuestra Senora de la Asunción

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    Lina Wolff

    2016-12-01

    Full Text Available Jednom sam upoznala pisca koji je rekao da više ne može podnijeti biti pisac. Bilo je to na zabavi u Madridu i ne sjećam se kako sam tamo završila, ali zabava je bila u Ulici Ventura de la Vega pa pretpostavljam da me netko koga sam upoznala te noći odveo tamo (moji prijatelji, ako sam ih uopće imala, živjeli su na sasvim drugim mjestima. Ako si pravi pisac, ne možeš samo tako prestati pisati, rekla sam. Moram, odgovorio je. Zato što se bojim da naginjem ludilu, a onih dana kad ne naginjem ludilu naginjem nečemu još gorem. Čemu?, upitala sam. Rekao je da ne zna, ali da mora misliti na ženu i dijete i da se, što se ludila tiče, slaže s Robertom Bolanom, da je zarazno. U to vrijeme nisam puno izlazila. Tek sam se udala i loše sam govorila španjolski, sin je bio samo godinu dana star i sve sam vrijeme provodila kod kuće, osim, katkad, kad bi mi se muž vratio s puta, spustio torbe u predsoblju i pogledao me dok sam sjedila na kauču nakon cijelog dana buljenja u sapunice. Mora da sam izgledala podbuhlo i zavidno dok sam tako sjedila na kauču kad bi se on vratio kući. Uvijek je nosio kravatu i sjajno odijelo i dok bi tamo stajao sa svojom crnom, španjolskom kosom, činilo mi se da u njegovim očima vidim sve zračne luke ovog svijeta. Ali nikada nije rekao da je stan u neredu ili da izgledam kao da tjedan dana nisam oprala kosu. Rekao je: Sad je na tebi red da malo izađeš. I podigao bi našeg sina, koji bi počeo vrištati. Naš bi mu sin ispovraćao velike žute mrlje po odijelu, ali moj muž samo bi se nasmijao i izgledao sretno. Španjolci vole djecu. Dobro se odijevaju i opušteni su, ta mi se kombinacija oduvijek sviđala.

  18. Snakebites notified to the poison control center of Morocco between 2009 and 2013.

    Science.gov (United States)

    Chafiq, Fouad; El Hattimy, Faiçal; Rhalem, Naima; Chippaux, Jean-Philippe; Soulaymani, Abdelmajid; Mokhtari, Abdelrhani; Soulaymani-Bencheikh, Rachida

    2016-01-01

    Snakebites cause considerable death and injury throughout the globe, particularly in tropical regions, and pose an important yet neglected threat to public health. In 2008, the Centre Anti Poison et de Parmacovigilance du Maroc (CAPM) started to set up a specific strategy for the control of snakebites that was formalized in 2012. The aim of the present study is to describe and update the epidemiological characteristics of snakebites notified to CAPM between 2009 and 2013. This retrospective five-year study included all cases of snakebites notified to CAPM by mail or phone. During the study period, 873 snakebite cases were reported to CAPM, an average incidence of 2.65 cases per 100,000 inhabitants with 218 cases each year. The highest incidence was found in Tangier-Tetouan region with 357 cases (40.9 %) followed by Souss Massa Draa region with 128 cases (14.6 %). The average age of patients was 26.8 ± 17.2 years. The male to female sex ratio was 1.67:1 and 77 % of cases occurred in rural areas. The bites occurred mainly in spring (44 %) followed by summer (42 %). Snake species was identified in 54 cases (6.2 %): colubrids represented 31 % (n = 18) and vipers 67 % (n = 36), mainly Daboia mauritanica, Bitis arietans and Cerastes cerastes. In 311 cases (35.6 %), the patients showed viper syndrome. Thrombocytopenia was observed in 23.5 % of viper syndrome cases, whereas, compartment syndrome was observed in 7.6 % patients. FAV-Afrique® was administered in 41 patients (5 %). In patients treated with antivenom, 38 patients recovered and three died. Twenty-seven deaths were reported (3.9 %). Despite specific efforts to better understand the epidemiology of snakebites in Morocco (incidence, severity, snake species involved), it remains underestimated. Therefore, further work is still necessary to ensure accessibility of appropriate antivenom against venomous species and to improve the management of envenomation in Morocco.

  19. UPOREDNI PREGLED ĆELIJSKIH PUTEVA I BIOHEMIJSKIH EFEKATA JONA HROMA

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    Andi Alijagić

    2017-07-01

    Full Text Available Efekti hroma kao suplementa ishrani, istraživani su dosada na brojnim životinjama. Većina studija je koristila hrom-pikolinat ili hrom hlorid kao izvor hroma, dok su hrom-nikotinat ili kvasac obogaćen hromom korišteni u samo nekoliko studija. Adekvatna preporuka u pogledu količine Cr(III još uvijek ne postoji, jer su efekti djelovanja hroma koji je dodat kao suplement ishrani životinja vrlo kontradiktorni. Trovalentni hrom je na neke životinje imao pozitivne efekte, a na neke štetne. Za šestovalentni hrom se sa sigurnošću zna da predstavlja izuzetno toksičnu supstancu i nipošto ne bi trebao biti dio ishrane. Nakon primjene Cr(VI, vrijednosti alanin aminotransferaze i sukcinat dehidrogenaze rastu nekoliko puta u odnosu na referentne vrijednosti. Koncentracija kreatin kinaze se dvostruko povećava. U većini studija koncentracija žučnih kiselina i holesterola raste u odnosu na kontrolnu grupu, dok u drugim studijama dolazi do pada koncentracije holesterola i triglicerida. Hrom ima veliki uticaj na signalne puteve koji su povezani sa unosom i metabolizmom glukoze. Hrom povećava kinaznu aktivnost inzulinskog receptora β, prilikom čega se povećava aktivnost nizvodnih efektora inzulinskog signaliziranja, poput PI3 kinaze i Akt serin/ treonin kinaze, što za posljedicu ima povećanu translokaciju Glut4 receptora na površinu ćelije. Povećana ekspresija transkripata gena za glutation sintazu, hem oksigenazu 2 i peroksiredoksin je utvrđena u ćelijama tretiranim hromom. Dodatno, hrom inhibira hidrogen-peroksidom posredovanu oksidaciju tiola i lipidnu peroksidaciju. Sumarno, oksidacija Cr(III jedinjenja vodi nastanku Cr(V i Cr(VI vrsta koje mogu reagirati sa cisteinskim reziduama PTP-a, što vodi povećanju nivoa fosforilacije tirozina i alteracijama u ćelijskom signaliziranju, uključujući povećanje aktivnosti receptora za inzulin.

  20. Bespilotne letelice zapadnih zemalja / Unmanned aircraft of Western countries

    Directory of Open Access Journals (Sweden)

    Slavko Pokorni

    2002-11-01

    Full Text Available Trend sve češće primene bespilotnih letelica biće nastavljen nesumnjivo, i tokom ove decenije. U vezi s tim stiče se utisak da će razvoj borbenih bespilotnih letilica biti u usponu. Mada je u proteklom periodu težište bilo na razvoju bespilotnih letilica za vojne primene (gde su ulagana velika sredstva, a civilni sektor je, uglavnom, koristio rezultate razvoja vojnih bespilotnih letelica, u narednom periodu se očekuje porast ulaganja i u razvoj bespilotnih letelica u civilnom sektoru. Bespilotne letelice su imale značajnu ulogu u zadacima koje su obavljale multinacionalne snage u toku rata u Bosni i Hercegovini i agresije NATO-a na SRJ, pa je poznavanje karakteristika bespilotnih letelica, za pripadnike Vojske, od velikog značaja. U sažetom tabelarnom pregledu prikazani su podatci o bespilotnim letelicama uglavnom proizvođača iz zapadnih zemalja, što ne znači da ih ne proizvode i druge zemlje, posebno Ruska federacija kao i neke susedne zemlje (Bugarska, Hrvatska. / The increasingly frequent use of unmanned aircraft will continue unabated throughout this decade. About that the impression is that the development of combat drones will rise. Although in the past period the focus was on the development of unmanned military vehicles (where large funds were invested, and the civil sector used mainly the development of military drones, in the coming period, investment in the development of unmanned aircraft in the civil sector is expected . Unmanned aircraft played a significant role in the tasks performed by multinational forces during the war in Bosnia and Herzegovina and NATO aggression in the FR Yugoslavia, so the knowledge of the characteristics of drones for members of the Army is of great importance. The summary table shows the data on unmanned aircraft mainly manufactured from Western countries, which does not mean that they are not produced by other countries, especially the Russian Federation as well as some neighboring

  1. Randomised controlled double-blind non-inferiority trial of two antivenoms for saw-scaled or carpet viper (Echis ocellatus envenoming in Nigeria.

    Directory of Open Access Journals (Sweden)

    Isa S Abubakar

    Full Text Available BACKGROUND: In West Africa, envenoming by saw-scaled or carpet vipers (Echis ocellatus causes great morbidity and mortality, but there is a crisis in supply of effective and affordable antivenom (ISRCTN01257358. METHODS: In a randomised, double-blind, controlled, non-inferiority trial, "EchiTAb Plus-ICP" (ET-Plus equine antivenom made by Instituto Clodomiro Picado was compared to "EchiTAb G" (ET-G ovine antivenom made by MicroPharm, which is the standard of care in Nigeria and was developed from the original EchiTAb-Fab introduced in 1998. Both are caprylic acid purified whole IgG antivenoms. ET-G is monospecific for Echis ocellatus antivenom (initial dose 1 vial and ET-Plus is polyspecific for E. ocellatus, Naja nigricollis and Bitis arietans (initial dose 3 vials. Both had been screened by pre-clinical and preliminary clinical dose-finding and safety studies. Patients who presented with incoagulable blood, indicative of systemic envenoming by E. ocellatus, were recruited in Kaltungo, north-eastern Nigeria. Those eligible and consenting were randomly allocated with equal probability to receive ET-Plus or ET-G. The primary outcome was permanent restoration of blood coagulability 6 hours after the start of treatment, assessed by a simple whole blood clotting test repeated 6, 12, 18, 24 and 48 hr after treatment. Secondary (safety outcomes were the incidences of anaphylactic, pyrogenic and late serum sickness-type antivenom reactions. FINDINGS: Initial doses permanently restored blood coagulability at 6 hours in 161/194 (83.0% of ET-Plus and 156/206 (75.7% of ET-G treated patients (Relative Risk [RR] 1.10 one-sided 95% CI lower limit 1.01; P = 0.05. ET-Plus caused early reactions on more occasions than did ET-G [50/194 (25.8% and 39/206 (18.9% respectively RR (1.36 one-sided 95% CI 1.86 upper limit; P = 0.06. These reactions were classified as severe in 21 (10.8% and 11 (5.3% of patients, respectively. CONCLUSION: At these doses, ET-Plus was

  2. Café Modra propast

    Directory of Open Access Journals (Sweden)

    Andrea Rožić

    2013-12-01

    Full Text Available Mamica se opet bavila svojim brkovima, jak miris voska, kovrče pare iznad lonca. Bila je grozno opsjednuta uklanjanjem dlaka; ovo joj je bilo drugi put u tjedan dana. Pribor razbacan po pultu u prednjem dijelu kafea, ljepljive posudice voska među prašnjavim staklenkama za slatkiše, drveni štapići vire iz posude za žličice za kavu. "Uz malo sreće, ti ovo nećeš morati raditi", rekla je nanoseći zlaćanu smjesu iznad gornje usnice. Pogledala se u zrcalu na reklami Coca-Cole koja je visjela na zidu s brodskim podom i pritisnula komad gaze na usta dok se tekućina ne stvrdne. "Vidi me, ja sam Sicilijanka. Prekrivena dlakama koje ne želim, kao Turkinje, kao Grkinje. Ali ti si Irkinja, Majella, i bjelja od toga ne možeš biti. 'Trebaju ti samo dvije generacije da se asimiliraš.' To mi je rekao tvoj stari nono kad si se rodila. 'Sad si Irkinja, Bonfilia. I imaš irsku bambinu kao dokaz.'" Uprla je štapićem u mene. "Ali nisam postala plavuša zbog tebe, je l' da?" Moj je nono sjedio u stražnjem dijelu prostorije, u separeu uz prozor. Ustajao je u četiri svakog jutra, dolazio u gaćama i naopako okrenutom vunenom džemperu i sjedio u kafeu. Nije se micao do osam uvečer, jeo je mliječne kekse i smrdio na pišalinu, kako obično mirišu devedesetogodišnjaci. Ponekad si je umišljao stvari; vikao na ljude kojih nema, bijesno mahao rukama. Mamica je rekla da ponovno proživljava dane u talijanskoj mornarici kada se borio u Španjolskom građanskom ratu. Uopće nije obraćao pažnju na nas jer se u njegovoj šašavoj glavi još nismo ni rodile. Ni mi nismo previše obraćale pažnju na njega, pa zato nismo ni primijetile da je prošli tjedan imao drugi moždani udar.

  3. Susceptibility of Diabetic Heart to Catecholamine-induced Arrhythmias is Independent of Contractile Dysfunction

    Directory of Open Access Journals (Sweden)

    Adameova Adriana

    2014-06-01

    čanih smrti u dijabetesu ne mora biti povezana sa odgovorom na kateholamine.

  4. Gezgin satıcı problemi için yeni bir meta-sezgisel: kör fare algoritması

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    Tevfik YILDIRIM

    2016-02-01

    Full Text Available Gezgin Satıcı Problemi (GSP, başlangıç ve bitiş şehirleri aynı olan ve her şehrin sadece bir kez ziyaret edildiği minimum mesafeli turu bulma problemidir. Şehir sayısı arttıkça, kesin yöntemler ile kabul edilebilir sürelerde bir optimum çözüm bulunması zordur. Bu nedenle, son elli yılda GSP’nin çözümü için doğadan ve biyolojiden esinlenen birçok meta-sezgisel yöntem geliştirilmiştir. Bu çalışmada, toprak altındaki bireysel tünel sistemlerinde yaşayan kör farelerin toprak altındaki engelleri geçme stratejisinden esinlenilerek GSP’nin çözümü için yeni bir meta-sezgisel tasarlanmıştır. Geliştirilen yönteme Kör Fare Algoritması adı verilmiştir. Bu yeni sezgisel ile farklı boyutlardaki simetrik test veri setleri için deneyler yapılmış ve sonuçları bilinen en iyi sonuçlar ile kıyaslanmıştır. Önerilen meta-sezgisel henüz literatürdeki diğer algoritmalarla yarışabilecek düzeyde olmamasına rağmen, başlangıç test çözümlerinin umut verici olduğu söylenebilir.Anahtar Kelimeler: Gezgin satıcı problemi, Kombinatoryel eniyileme, Meta-Sezgisel, Kör fare algoritması

  5. Poliklorirani bifenili – toksičnost i rizici

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    Ivana Kmetič

    2012-01-01

    Full Text Available Poliklorirani bifenili (PCB-i pripadaju velikoj skupini sintetskih organokloriranih spojeva koji se međusobno razlikuju u fizikalno–kemijskim i toksikološkim svojstvima te imaju tendenciju bioakumulacije i biomagnifikacije u okolišu i unutar hranidbenih lanaca. Uslijed toga, ljudi mogu biti izloženi PCB-ima preko kontaminirane vode i hrane (riba, meso i mliječni proizvodi. Proizvodnja i uporaba PCB-a je određenim zakonskim propisima ograničena u većini zemalja Europske unije, dok je u nekima u potpunosti zabranjena. Međutim, u Republici Hrvatskoj rizik je još uvijek prisutan zbog vojnih razaranja tijekom Domovinskog rata (1991.-1995. u kojem su oštećeni ili uništeni mnogi elektroenergetski, industrijski i drugi objekti iz kojih je došlo do istjecanja PCB-a. Postoji potreba provedbe daljnjih aktivnosti na sanaciji kontaminiranih lokacija i zbrinjavanju transformatora i kondenzatora u kojima su korišteni PCB-i te je nužno uspostaviti monitoring i ostalih postojanih organskih onečišćujućih tvari (POP-s u okolišu sukladno provedbi Stockholmske konvencije čija je supotpisnica i Republika Hrvatska. Dosadašnja znanstvena istraživanja ukazuju na razne negativne učinke PCB-a na ljudsko zdravlje. Dokazano je da PCB-i mogu poremetiti endokrinu homeostazu organizma i izazvati reprodukcijsku toksičnost te da djeluju imunotoksično, neurotoksično, genotoksično. Stupanj toksičnosti pojedinog PCB kongenera uvelike ovisi o poziciji i broju supstituiranih atoma klora, pri čemu se planarni PCB-i koji u orto položaju ne sadrže atome klora ( ili sadrže svega jedan atom klora smatraju toksičnijima. Strukturna sličnost između planarnih kongenera i dioksina objašnjava njihov mehanizam djelovanja posredovan aril ugljikovodičnim receptorom (AhR, dok su neplanarni PCB-i slabi antagonisti AhR-a. Treba napomenuti da svi toksični efekti ne ovise o Ah-receptoru te PCB-i koji nemaju planarnu strukturu i koji su orto supstituirani mogu

  6. Bazı Uçucu Yağların Myzus persicae ve Avcısı Chrysoperla carnea Üzerindeki Etkileri

    Directory of Open Access Journals (Sweden)

    Ali KAYAHAN

    2016-06-01

    Full Text Available Bu çalışmada, laboratuvar koşullarında farklı bitkisel uçucu yağların Şeftali yeşil yaprakbiti Myzus persicae (Sulz. (Hemiptera: Aphididae ve bu zararlının avcısı olan Chrysoperla carnea (Stephens (Neuroptera: Chrysopidae üzerindeki etkisi belirlenmiştir. Denemeler 25±1° C sıcaklığa, %60±5 ve 16:8 aydınlatma koşullarına sahip iklim odalarında gerçekleştirilmiştir. Yaprak biti bireyleri biber bitkileri (Capsicum annuum L. üzerinde üretilirken, avcılar bu afit ile beslenmiştir.  Bitkisel yağların etkileri püskürtme yöntemi ile araştırılmıştır. Kontrol uygulamalarında saf su kullanılmıştır. Uygulamadan 1., 3., 5. ve 7. günden sonra ölü ve canlı bireyler sayılmıştır. Bitkisel yağların etkisi Abbott formülü kullanılarak değerlendirilmiştir. Denemeler 5 tekerrürlü olarak yürütülmüştür. Elde edilen sonuçlara göre; karanfil, lavanta ve ardıcın M. persicae üzerindeki 7. gündeki etkisi sırasıyla %78.26, %69.57 ve %47.83 olarak bulunmuştur. Uygulanan tüm uçucu yağların C. carnea için zararsız olduğu görülmüştür.

  7. Snakes and snakebite envenoming in Northern Tanzania: a neglected tropical health problem.

    Science.gov (United States)

    Kipanyula, M J; Kimaro, W H

    2015-01-01

    Snakebites cause considerable human and livestock injuries as well as deaths worldwide, and particularly have a high impact in sub-Saharan Africa. Generating a basic platform of information on the characteristics of snakes and snakebites in various countries is relevant for designing and implementing public health interventions. This study was performed to identify types of snakes and some of the characteristics of snakebite cases in two communities, an agricultural and a pastoralist, in Arusha region, northern Tanzania. A total of 30 field visits were carried out in areas considered by local inhabitants to be potential microhabitats for snakes. Direct observation of snake types based on morphological features and a structured questionnaire were employed for data collection. A total of 25 live and 14 dead snakes were encountered. Among the dead ones, the following species were identified: two black-necked spitting cobras (Naja nigricollis); five puff adders (Bitis arietans), one common egg-eater (Dasypeltis scabra); two rufous-beaked snakes (Ramphiophis rostratus); two brown house snakes (Lamprophis fuliginosus); one Kenyan sand boa (Eryx colubrinus), and one black mamba (Dendroaspis polylepis). The frequency of snake encounters was significantly higher (χ (2) = 4.6; p = 0.03) in the pastoral than in the agricultural area; there were more snakebite cases in the former, but the differences were not statistically significant (p = 0.7). A total of 242 snakebite victims attended at the Meserani Clinic, located in the study area, between the years 2007 to 2012. Of all cases, 146 (61.6 %) and 96 (38.4 %) were male and female patients, respectively. As for age distribution, 59.1 % of snakebite victims were from the economically active age groups between 15 and 55 years. Snakebites are a threat to rural communities and public health in general. The burden of snakebites in Tanzania presents an epidemiologically similar picture to other tropical countries

  8. Protein and Lipid Concentrations in Patients with Differentiated Thyroid Cancer Treated with Radioactive Iodine-131 / Koncentracija Proteina I Lipida Kod Pacijenata Sa Diferentovanim Karcinomom Štitaste Žlezde Koji Su Lečeni Radioaktivnim Jodom-131

    Directory of Open Access Journals (Sweden)

    Mihaljevic Olgica

    2014-12-01

    Full Text Available Prolazna, manifestna hipotireoza koja se javlja kod pacijenata sa diferentovanim karcinomom štitaste žlezde (DTC pre terapije radioaktivnim jodom (131-I može biti udružena sa brojnim metaboličkim promenama, uključujući i promene u metabolizmu proteina i lipida. Koncentracija proteina i njihov odnos sa lipidima u serumu pacijenata sa DTC nakon terapije 131-I nedovoljno su ispitani. Cilj našeg istraživanja bio je da se ispita serumska koncentracija proteina i lipida kod pacijenata sa DTC pre, kao i tri i sedam dana posle terapije 131-I. Studijom je obuhvaćeno 24 DTC pacijenata i 20 zdravih ispitanika. Pokazano je značajno, progresivno smanjenje koncentracije proteina (78.71±6.71 g/L vs. 87.16±6.04 g/L; p=0.003 i holesterola (8.12±2.13 mmol/L vs. 8.84±2.09 mmol/L; p=0.001 tri dana nakon terapije 131-I, uz statistički značajno povećanje koncentracije triglicerida tri dana nakon terapije (2.44±1.07 mmol/L vs. 2.26±1.08 mmol/L; p=0.041 i povratkom na preterapijske vrednosti 7 dana posle terapije. Pri tom, indirektna korelacija između koncentracije proteina i triglicerida tri dana posle 131-I pokazana je u grupi pacijenata starijih od 50 godina (Spearman r=- 0.583, p=0.048, što nije bio slučaj sa ispitanicima mlađim od 50 godina (Pearson r=- 0.277, p=0.384. U zaključku, terapija radioaktivnim jodom prouzrokuje smanjenje koncentracije serumskih proteina i holesterola, koje je udruženo sa povećanjem koncentracije triglicerida i posebno je izraženo kod starijih pacijenata sa metastazama.

  9. Testing the effects of perimeter fencing and elephant exclosures on lion predation patterns in a Kenyan wildlife conservancy

    Directory of Open Access Journals (Sweden)

    Marc Dupuis-Desormeaux

    2016-02-01

    Full Text Available The use of fences to segregate wildlife can change predator and prey behaviour. Predators can learn to incorporate fencing into their hunting strategies and prey can learn to avoid foraging near fences. A twelve-strand electric predator-proof fence surrounds our study site. There are also porous one-strand electric fences used to create exclosures where elephant (and giraffe cannot enter in order to protect blocs of browse vegetation for two critically endangered species, the black rhinoceros (Diceros bicornis and the Grevy’s zebra (Equus grevyi. The denser vegetation in these exclosures attracts both browsing prey and ambush predators. In this study we examined if lion predation patterns differed near the perimeter fencing and inside the elephant exclosures by mapping the location of kills. We used a spatial analysis to compare the predation patterns near the perimeter fencing and inside the exclosures to predation in the rest of the conservancy. Predation was not over-represented near the perimeter fence but the pattern of predation near the fence suggests that fences may be a contributing factor to predation success. Overall, we found that predation was over-represented inside and within 50 m of the exclosures. However, by examining individual exclosures in greater detail using a hot spot analysis, we found that only a few exclosures contained lion predation hot spots. Although some exclosures provide good hunting grounds for lions, we concluded that exclosures did not necessarily create prey-traps per se and that managers could continue to use this type of exclusionary fencing to protect stands of dense vegetation.

  10. Testing the effects of perimeter fencing and elephant exclosures on lion predation patterns in a Kenyan wildlife conservancy.

    Science.gov (United States)

    Dupuis-Desormeaux, Marc; Davidson, Zeke; Pratt, Laura; Mwololo, Mary; MacDonald, Suzanne E

    2016-01-01

    The use of fences to segregate wildlife can change predator and prey behaviour. Predators can learn to incorporate fencing into their hunting strategies and prey can learn to avoid foraging near fences. A twelve-strand electric predator-proof fence surrounds our study site. There are also porous one-strand electric fences used to create exclosures where elephant (and giraffe) cannot enter in order to protect blocs of browse vegetation for two critically endangered species, the black rhinoceros (Diceros bicornis) and the Grevy's zebra (Equus grevyi). The denser vegetation in these exclosures attracts both browsing prey and ambush predators. In this study we examined if lion predation patterns differed near the perimeter fencing and inside the elephant exclosures by mapping the location of kills. We used a spatial analysis to compare the predation patterns near the perimeter fencing and inside the exclosures to predation in the rest of the conservancy. Predation was not over-represented near the perimeter fence but the pattern of predation near the fence suggests that fences may be a contributing factor to predation success. Overall, we found that predation was over-represented inside and within 50 m of the exclosures. However, by examining individual exclosures in greater detail using a hot spot analysis, we found that only a few exclosures contained lion predation hot spots. Although some exclosures provide good hunting grounds for lions, we concluded that exclosures did not necessarily create prey-traps per se and that managers could continue to use this type of exclusionary fencing to protect stands of dense vegetation.

  11. Feather corticosterone reveals stress associated with dietary changes in a breeding seabird.

    Science.gov (United States)

    Will, Alexis; Watanuki, Yutaka; Kikuchi, Dale M; Sato, Nobuhiko; Ito, Motohiro; Callahan, Matt; Wynne-Edwards, Katherine; Hatch, Scott; Elliott, Kyle; Slater, Leslie; Takahashi, Akinori; Kitaysky, Alexander

    2015-10-01

    Changes in climate and anthropogenic pressures might affect the composition and abundance of forage fish in the world's oceans. The junk-food hypothesis posits that dietary shifts that affect the quality (e.g., energy content) of food available to marine predators may impact their physiological state and consequently affect their fitness. Previously, we experimentally validated that deposition of the adrenocortical hormone, corticosterone, in feathers is a sensitive measure of nutritional stress in seabirds. Here, we use this method to examine how changes in diet composition and prey quality affect the nutritional status of free-living rhinoceros auklets (Cerorhinca monocerata). Our study sites included the following: Teuri Is. Japan, Middleton Is. central Gulf of Alaska, and St. Lazaria Is. Southeast Alaska. In 2012 and 2013, we collected "bill loads" delivered by parents to feed their chicks (n = 758) to document dietary changes. We deployed time-depth-temperature recorders on breeding adults (n = 47) to evaluate whether changes in prey coincided with changes in foraging behavior. We measured concentrations of corticosterone in fledgling (n = 71) and adult breeders' (n = 82) feathers to determine how birds were affected by foraging conditions. We found that seasonal changes in diet composition occurred on each colony, adults dove deeper and engaged in longer foraging bouts when capturing larger prey and that chicks had higher concentrations of corticosterone in their feathers when adults brought back smaller and/or lower energy prey. Corticosterone levels in feathers of fledglings (grown during the breeding season) and those in feathers of adult breeders (grown during the postbreeding season) were positively correlated, indicating possible carryover effects. These results suggest that seabirds might experience increased levels of nutritional stress associated with moderate dietary changes and that physiological responses to changes in prey composition

  12. SU-F-J-174: A Series of Computational Human Phantoms in DICOM-RT Format for Normal Tissue Dose Reconstruction in Epidemiological Studies

    International Nuclear Information System (INIS)

    Pyakuryal, A; Moroz, B; Lee, C; Pelletier, C; Jung, J; Lee, C

    2016-01-01

    Purpose: Epidemiological studies of second cancer risk in radiotherapy patients often require individualized dose estimates of normal tissues. Prior to 3D conformal radiation therapy planning, patient anatomy information was mostly limited to 2D radiological images or not even available. Generic patient CT images are often used in commercial radiotherapy treatment planning system (TPS) to reconstruct normal tissue doses. The objective of the current work was to develop a series of reference size computational human phantoms in DICOM-RT format for direct use in dose reconstruction in TPS. Methods: Contours of 93 organs and tissues were extracted from a series of pediatric and adult hybrid computational human phantoms (newborn, 1-, 5-, 10-, 15-year-old, and adult males and females) using Rhinoceros software. A MATLAB script was created to convert the contours into the DICOM-RT structure format. The simulated CT images with the resolution of 1×1×3 mm3 were also generated from the binary phantom format and coupled with the DICOM-structure files. Accurate volumes of the organs were drawn in the format using precise delineation of the contours in converted format. Due to complex geometry of organs, higher resolution (1×1×1 mm3) was found to be more efficient in the conversion of newborn and 1-year-old phantoms. Results: Contour sets were efficiently converted into DICOM-RT structures in relatively short time (about 30 minutes for each phantom). A good agreement was observed in the volumes between the original phantoms and the converted contours for large organs (NRMSD<1.0%) and small organs (NRMSD<7.7%). Conclusion: A comprehensive series of computational human phantoms in DICOM-RT format was created to support epidemiological studies of second cancer risks in radiotherapy patients. We confirmed the DICOM-RT phantoms were successfully imported into the TPS programs of major vendors.

  13. Effect of different types of prosthetic platforms on stress-distribution in dental implant-supported prostheses.

    Science.gov (United States)

    Minatel, Lurian; Verri, Fellippo Ramos; Kudo, Guilherme Abu Halawa; de Faria Almeida, Daniel Augusto; de Souza Batista, Victor Eduardo; Lemos, Cleidiel Aparecido Araujo; Pellizzer, Eduardo Piza; Santiago, Joel Ferreira

    2017-02-01

    A biomechanical analysis of different types of implant connections is relevant to clinical practice because it may impact the longevity of the rehabilitation treatment. Therefore, the objective of this study is to evaluate the Morse taper connections and the stress distribution of structures associated with the platform switching (PSW) concept. It will do this by obtaining data on the biomechanical behavior of the main structure in relation to the dental implant using the 3-dimensional finite element methodology. Four models were simulated (with each containing a single prosthesis over the implant) in the molar region, with the following specifications: M1 and M2 is an external hexagonal implant on a regular platform; M3 is an external hexagonal implant using PSW concept; and M4 is a Morse taper implant. The modeling process involved the use of images from InVesalius CT (computed tomography) processing software, which were refined using Rhinoceros 4.0 and SolidWorks 2011 CAD software. The models were then exported into the finite element program (FEMAP 11.0) to configure the meshes. The models were processed using NeiNastram software. The main results are that M1 (regular diameter 4mm) had the highest stress concentration area and highest microstrain concentration for bone tissue, dental implants, and the retaining screw (Pstress concentrations in the retaining screw (Pstress distribution and that the PSW concept had higher stress concentrations in the retaining screw and the crown compared to the regular platform implant. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. SU-E-T-61: A Practical Process for Fabricating Passive Scatter Proton Beam Modulation Compensation Filters Using 3D Printing

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, T; Drzymala, R [Washington University School of Medicine, St. Louis, MO (United States)

    2015-06-15

    Purpose: The purpose of this project was to devise a practical fabrication process for passive scatter proton beam compensation filters (CF) that is competitive in time, cost and effort using 3D printing. Methods: DICOM compensator filter files for a proton beam were generated by our Eclipse (Varian, Inc.) treatment planning system. The compensator thickness specifications were extracted with in-house software written in Matlab (MathWorks, Inc.) code and written to a text file which could be read by the Rhinoceros 5, computer-aided design (CAD) package (Robert McNeel and Associates), which subsequently generated a smoothed model in a STereoLithographic also known as a Standard Tesselation Language file (STL). The model in the STL file was subsequently refined using Netfabb software and then converted to printing instructions using Cura. version 15.02.1. for our 3D printer. The Airwolf3D, model HD2x, fused filament fabrication (FFF) 3D printer (Airwolf3D.com) was used for our fabrication system with a print speed of 150mm per second. It can print in over 22 different plastic filament materials in a build volume of 11” x 8” x 12”. We choose ABS plastic to print the 3D model of the imprint for our CFs. Results: Prints of the CF could be performed at a print speed of 70mm per second. The time to print the 3D topology for the CF for the 14 cm diameter snout of our Mevion 250 proton accelerator was less than 3 hours. The printed model is intended to subsequently be used as a mold to imprint a molten wax cylindrical to form the compensation after cooling. The whole process should be performed for a typical 3 beam treatment plan within a day. Conclusion: Use of 3D printing is practical and can be used to print a 3D model of a CF within a few hours.

  15. Reproductive tract tumours: the scourge of woman reproduction ails Indian rhinoceroses.

    Directory of Open Access Journals (Sweden)

    Robert Hermes

    Full Text Available In Indian rhinoceros, extensive leiomyoma, a benign smooth muscle tumour, was sporadically diagnosed post mortem and commonly thought of as contributing factor for reduced fecundity of this species in captivity. However, to date, the prevalence of reproductive tract tumours and their relevance for fecundity are unknown. Our analysis of the international studbook now reveals that females cease reproducing at the age of 18.1±1.2 years; equivalent to a reproductive lifespan of just 9.5±1.3 years. This short reproductive life is in sharp contrast to their longevity in captivity of over 40 years. Here we show, after examining 42% of the captive female population, that age-related genital tract tumours are highly prevalent in this endangered species. Growth and development of these tumours was found to be age-related, starting from the age of 10 years. All females older than 12 years had developed genital tumours, just 7-9 years past maturity. Tumour sizes ranged from 1.5-10 cm. With age, tumours became more numerous, sometimes merging into one large diffuse tumour mass. These tumours, primarily vaginal and cervical, presumably cause widespread young-age infertility by the age of 18 years. In few cases, tumour necrosis suggested possible malignancy of tumours. Possible consequences of such genital tract tumour infestation are hindered intromission, pain during mating, hampered sperm passage, risk of ascending infection during pregnancy, dystocia, or chronic vaginal bleeding. In humans, leiomyoma affect up to 80% of pre-menopause women. While a leading cause for infertility, pregnancy is known to reduce the risk of tumour development. However, different from human, surgical intervention is not a viable treatment option in rhinoceroses. Thus, in analogy to humans, we suggest early onset and seamless consecutive pregnancies to help reduce prevalence of this disease, better maintain a self-sustained captive population and improve animal welfare.

  16. From Survey to FEM Analysis for Documentation of Built Heritage: the Case Study of Villa Revedin-Bolasco

    Science.gov (United States)

    Guarnieri, A.; Fissore, F.; Masiero, A.; Di Donna, A.; Coppa, U.; Vettore, A.

    2017-05-01

    In the last decade advances in the fields of close-range photogrammetry, terrestrial laser scanning (TLS) and computer vision (CV) have enabled to collect different kind of information about a Cultural Heritage objects and to carry out highly accurate 3D models. Additionally, the integration between laser scanning technology and Finite Element Analysis (FEA) is gaining particular interest in recent years for structural analysis of built heritage, since the increasing computational capabilities allow to manipulate large datasets. In this note we illustrate the approach adopted for surveying, 3D modeling and structural analysis of Villa Revedin-Bolasco, a magnificent historical building located in the small walled town of Castelfranco Veneto, in northern Italy. In 2012 CIRGEO was charged by the University of Padova to carry out a survey of the Villa and Park, as preliminary step for subsequent restoration works. The inner geometry of the Villa was captured with two Leica Disto D3a BT hand-held laser meters, while the outer walls of the building were surveyed with a Leica C10 and a Faro Focus 3D 120 terrestrial laser scanners. Ancillary GNSS measurements were also collected for 3D laser model georeferencing. A solid model was then generated from the laser global point cloud in Rhinoceros software, and portion of it was used for simulation in a Finite Element Analysis (FEA). In the paper we discuss in detail all the steps and challenges addressed and solutions adopted concerning the survey, solid modeling and FEA from laser scanning data of the historical complex of Villa Revedin-Bolasco.

  17. Fatigue surviving, fracture resistance, shear stress and finite element analysis of glass fiber posts with different diameters.

    Science.gov (United States)

    Wandscher, Vinícius Felipe; Bergoli, César Dalmolin; de Oliveira, Ariele Freitas; Kaizer, Osvaldo Bazzan; Souto Borges, Alexandre Luiz; Limberguer, Inácio da Fontoura; Valandro, Luiz Felipe

    2015-03-01

    This study evaluated the shear stress presented in glass fiber posts with parallel fiber (0°) and different coronal diameters under fatigue, fracture resistance and FEA. 160 glass-fiber posts (N=160) with eight different coronal diameters were used (DT=double tapered, number of the post=coronal diameter and W=Wider - fiber post with coronal diameter wider than the conventional): DT1.4; DT1.8W; DT1.6; DT2W; DT1.8; DT2.2W; DT2; DT2.2. Eighty posts were submitted to mechanical cycling (3×10(6) cycles; inclination: 45°; load: 50N; frequency: 4Hz; temperature: 37°C) to assess the surviving under intermittent loading and other eighty posts were submitted to fracture resistance testing (resistance [N] and shear-stress [MPa] values were obtained). The eight posts types were 3D modeled (Rhinoceros 4.0) and the shear-stress (MPa) evaluated using FEA (Ansys 13.0). One-way ANOVA showed statistically differences to fracture resistance (DT2.2W and DT2.2 showed higher values) and shear stress values (DT1.4 showed lower values). Only the DT1.4 fiber posts failed after mechanical cycling. FEA showed similar values of shear stress between the groups and these values were similar to those obtained by shear stress testing. The failure analysis showed that 95% of specimens failed by shear. Posts with parallel fiber (0°) may suffer fractures when an oblique shear load is applied on the structure; except the thinner group, greater coronal diameters promoted the same shear stresses. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Simple diagnostic signs in those with cognitive impairment-anything to improve detection of cognitive disorders.

    Science.gov (United States)

    Woodward, Michael C

    2017-11-01

    With now over 50 million people worldwide with dementia (Prince et al., 2013), there are almost certainly well over 100 million people with cognitive concerns and many of these will attend their health professional keen to know what is going on. We need those without intensive training in this field to be more confident and correct in their diagnosis when such a concerned person turns up. Many simple diagnostic tests have been proposed and some assessed - these include the walk and talk (divided attention) test (those who stop when asked a question while walking may be cognitively impaired) (Lamoth et al., 2011), the clock drawing test (Brodaty and Moore, 1997), the "handbag" sign (those clutching their personal possessions are more likely to be cognitively impaired) and the "hippopotamus sign" (calling the rhinoceros, in those tests that include this, a hippopotamus). Simple screening tests have been extensively validated and are important to the clinician in formulating a diagnosis (Lorentz et al., 2002). The "head-turning" and the "attended with/alone" signs are frequently observed, and many clinicians assessing such individuals would be well aware of them and probably even unknowingly factor them into their diagnosis. In this issue, Pinar Soysal and colleagues (Soysal et al., 2017) have evaluated these signs and, in those older people attending with cognitive concerns, found they had quite good diagnostic value. They were not very specific but showed good sensitivity and negative predictive value. Indeed, at the recent Alzheimer's Association International Conference in London there were several posters evaluating "soft but simple" signs although not all performed as expected - one group found gait actually sped up in those with cognitive impairment that were asked a question.

  19. Otimização multiobjetivo das dimensões dos ambientes de uma residência unifamiliar baseada em simulação energética e estrutural

    Directory of Open Access Journals (Sweden)

    Ligiana Pricila Guimarães Fonseca

    Full Text Available Resumo O desenvolvimento da tecnologia computacional nas últimas décadas levou a otimização associada à parametrização a possibilitar o desenvolvimento de projetos de melhor desempenho, com ou sem integração com a simulação. O intuito deste trabalho foi aplicar a otimização baseada em simulação na forma de uma edificação residencial unifamiliar com o objetivo de encontrar a composição que apresentasse o melhor desempenho térmico com o menor custo dos materiais construtivos, considerando-se restrições estruturais. Foram utilizados os programas Rhinoceros e Energy Plus, além de plug-ins para modelagem paramétrica, o intercâmbio de informações entre os programas e a condução da otimização. Foram realizadas duas simulações, a primeira considerando os custos dos componentes construtivos e os graus-hora de aquecimento e resfriamento combinados e a segunda considerando os custos e graus-hora de aquecimento, que produziu resultados mais relevantes. Dois casos de cada solução de Pareto foram selecionados para análise, dentre 19,8 mil e 27,4 mil casos obtidos com uso de algoritmos evolucionários. Apesar das limitações de algumas das ferramentas, foi verificado um grande potencial de aplicação deste método no desenvolvimento de projetos arquitetônicos.

  20. Effect of different types of prosthetic platforms on stress-distribution in dental implant-supported prostheses

    International Nuclear Information System (INIS)

    Minatel, Lurian; Verri, Fellippo Ramos; Kudo, Guilherme Abu Halawa; Faria Almeida, Daniel Augusto de; Souza Batista, Victor Eduardo de; Aparecido Araujo Lemos, Cleidiel; Piza Pellizzer, Eduardo

    2017-01-01

    A biomechanical analysis of different types of implant connections is relevant to clinical practice because it may impact the longevity of the rehabilitation treatment. Therefore, the objective of this study is to evaluate the Morse taper connections and the stress distribution of structures associated with the platform switching (PSW) concept. It will do this by obtaining data on the biomechanical behavior of the main structure in relation to the dental implant using the 3-dimensional finite element methodology. Four models were simulated (with each containing a single prosthesis over the implant) in the molar region, with the following specifications: M1 and M2 is an external hexagonal implant on a regular platform; M3 is an external hexagonal implant using PSW concept; and M4 is a Morse taper implant. The modeling process involved the use of images from InVesalius CT (computed tomography) processing software, which were refined using Rhinoceros 4.0 and SolidWorks 2011 CAD software. The models were then exported into the finite element program (FEMAP 11.0) to configure the meshes. The models were processed using NeiNastram software. The main results are that M1 (regular diameter 4 mm) had the highest stress concentration area and highest microstrain concentration for bone tissue, dental implants, and the retaining screw (P < 0.05). Using the PSW concept increases the area of the stress concentrations in the retaining screw (P < 0.05) more than in the regular platform implant. It was concluded that the increase in diameter is beneficial for stress distribution and that the PSW concept had higher stress concentrations in the retaining screw and the crown compared to the regular platform implant. - Highlights: • The external hexagon implants was unfavorable biomechanical. • The Morse taper implant presented the best biomechanical result. • Platform switching concept increased stress in screw-retained prostheses.