WorldWideScience

Sample records for bisindole natural products

  1. Marine Natural Product Bis-indole Alkaloid Caulerpin: Chemistry and Biology.

    Science.gov (United States)

    Lunagariya, Jignesh; Bhadja, Poonam; Zhong, Shenghui; Vekariya, Rohit; Xu, Shihai

    2017-09-27

    Marine bis-indole alkaloids comprise a large and increasingly growing class of secondary metabolites, and continue to deliver a great variety of structural templates. The alkaloids derived from marine resources play a crucial role in medicinal chemistry and as chemical agents. In particular, bis-indole alkaloid caulerpin isolated from marine green algae Caulerpa and a red algae Chondria armata at various places around the world, and tested against several therapeutic areas such as anti-diabetic, antinociceptive, anti-inflammatory, anti-tumor, anti-larvicidal, anti-herpes, anti-tubercular, anti-microbial and immunostimulating activity as well as means of other chemical agents. Herein, we summarized discovery of caulerpin, and its potential medicinal and chemical applications in chronological order with various aspects. Additionally, synthesis of caulerpin, its functional analogues, and structural isomer have also been reviewed. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  2. Synthesis of Bisindole Alkaloids from the Apocynaceae Which Contain a Macroline or Sarpagine Unit: A Review.

    Science.gov (United States)

    Rahman, Md Toufiqur; Tiruveedhula, Veera V N Phani Babu; Cook, James M

    2016-11-14

    Bisindole natural products consist of two monomeric indole alkaloid units as their obligate constituents. Bisindoles are more potent with respect to their biological activity than their corresponding monomeric units. In addition, the synthesis of bisindoles are far more challenging than the synthesis of monomeric indole alkaloids. Herein is reviewed the enantiospecific total and partial synthesis of bisindole alkaloids isolated primarily from the Alstonia genus of the Apocynaceae family. The monomeric units belong to the sarpagine, ajmaline, macroline, vobasine, and pleiocarpamine series. An up-to-date discussion of their isolation, characterization, biological activity as well as approaches to their partial and total synthesis by means of both synthetic and biosynthetic strategies are presented.

  3. New and bioactive natural products isolated from madagascar plants and marine organisms.

    Science.gov (United States)

    Hou, Y; Harinantenaina, L

    2010-01-01

    Madagascar, the world's fourth biggest island has an unique biodiversity. The interest on the phytochemical investigation of Malagasy plants and marine natural products started from the isolation of the potent anti-cancerous bisindole alkaloids: vinblastine and vincristine. In this paper, works published in the last two decades (1991-2009) on 270 new natural products isolated from Madagascar higher plants, liverworts and marine organisms are reviewed. Several results on the bioassays of the isolated new natural products have been reported.

  4. Indirubin, a bisindole alkaloid from Isatis indigotica, reduces H1N1 susceptibility in stressed mice by regulating MAVS signaling.

    Science.gov (United States)

    Jie, Chong; Luo, Zhuo; Chen, Huan; Wang, Min; Yan, Chang; Mao, Zhong-Fu; Xiao, Gao-Keng; Kurihara, Hiroshi; Li, Yi-Fang; He, Rong-Rong

    2017-12-01

    Isatis indigotica has a long history in treating virus infection and related symptoms in China. Nevertheless, its antivirus evidence in animal studies is not satisfactory, which might be due to the lack of appropriate animal model. Previously, we had utilized restraint stress to establish mouse H1N1 susceptibility model which was helpful in evaluating the anti-virus effect of medicines targeting host factors, such as type I interferon production. In this study, this model was employed to investigate the effect and mechanism of indirubin, a natural bisindole alkaloid from Isatis indigotica , on influenza A virus susceptibility. In the in vitro study, the stress hormone corticosterone was used to simulate restraint stress. Our results demonstrated that indirubin decreased the susceptibility to influenza virus with lowered mortality and alleviated lung damage in restraint-stressed mice model. Moreover, indirubin promoted the expression of interferon-β and interferon inducible transmembrane 3. In addition, indirubin maintained the morphology and function of mitochondria following influenza A virus infection. Further study revealed that indirubin promoted interferon-β production through promoting mitochondrial antiviral signaling pathway. Our study indicated that indirubin could be a candidate for the therapy of influenza.

  5. PRISM 3: expanded prediction of natural product chemical structures from microbial genomes.

    Science.gov (United States)

    Skinnider, Michael A; Merwin, Nishanth J; Johnston, Chad W; Magarvey, Nathan A

    2017-07-03

    Microbial natural products represent a rich resource of pharmaceutically and industrially important compounds. Genome sequencing has revealed that the majority of natural products remain undiscovered, and computational methods to connect biosynthetic gene clusters to their corresponding natural products therefore have the potential to revitalize natural product discovery. Previously, we described PRediction Informatics for Secondary Metabolomes (PRISM), a combinatorial approach to chemical structure prediction for genetically encoded nonribosomal peptides and type I and II polyketides. Here, we present a ground-up rewrite of the PRISM structure prediction algorithm to derive prediction of natural products arising from non-modular biosynthetic paradigms. Within this new version, PRISM 3, natural product scaffolds are modeled as chemical graphs, permitting structure prediction for aminocoumarins, antimetabolites, bisindoles and phosphonate natural products, and building upon the addition of ribosomally synthesized and post-translationally modified peptides. Further, with the addition of cluster detection for 11 new cluster types, PRISM 3 expands to detect 22 distinct natural product cluster types. Other major modifications to PRISM include improved sequence input and ORF detection, user-friendliness and output. Distribution of PRISM 3 over a 300-core server grid improves the speed and capacity of the web application. PRISM 3 is available at http://magarveylab.ca/prism/. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  6. Natural product antifoulants

    Digital Repository Service at National Institute of Oceanography (India)

    Raveendran, T.V.; Mol, V.P.L.

    Natural Product Antifoulants (NPAs) have been proposed as one of the best replacement options for the most successful antifouling agent, tri-n-butyl tin (TBT), which, due to its ecological incompatibility, is currently facing total global ban...

  7. Natural products in endodontics.

    Science.gov (United States)

    Almadi, Ebtissam M; Almohaimede, Amal A

    2018-02-01

    Herbal remedies are used throughout the world, either in earlier or in recent times. The number of studies on this alternative therapeutic system increased in the last decades. In this paper, the relevant literature on the use of natural products in root canal therapy is revised from a MEDLINE database search. The uses of medicinal plants in endodontics include cleaning and disinfection of root canals, intracanal medicaments between appointments, sealer cements, and for removal of obturation material. Other studies showed the effect of natural products in pulpal and dentin repair. Their use is anecdotal, and their effectiveness showed to be variable and is always compared to the chemical standards currently being used. Alkaloids, coumarins, saponins, and flavonoids are aromatic substances that are produced by plants and evaluated for their therapeutic potential. Further investigation into benefits of natural products is warranted.

  8. Natural products in chemical biology

    National Research Council Canada - National Science Library

    Civjan, Natanya

    2012-01-01

    .... The book begins by introducing natural products from different origins, moves on to presenting and discussing biosynthesis of various classes of natural products, and then looks at natural products...

  9. Dragmacidins G and H, Bisindole Alkaloids Tethered by a Guanidino Ethylthiopyrazine Moiety, from a Lipastrotethya sp. Marine Sponge.

    Science.gov (United States)

    Hitora, Yuki; Takada, Kentaro; Ise, Yuji; Okada, Shigeru; Matsunaga, Shigeki

    2016-11-23

    LCMS analysis of the extract and a cytotoxicity assay of the HPLC fractions generated from a small-scale extract of a Lipastrotethya sp. marine sponge demonstrated the presence of bisindole alkaloids that were associated with the cytotoxic activity. Two bisindole alkaloids tethered by a guanidino ethylthiopyrazine moiety, dragmacidins G (1) and H (2), were isolated, and their structures were assigned by analysis of the MS and NMR data. They showed moderate cytotoxic activity against HeLa cells.

  10. Natural products as photoprotection.

    Science.gov (United States)

    Saewan, Nisakorn; Jimtaisong, Ampa

    2015-03-01

    The rise in solar ultraviolet radiation on the earth's surface has led to a depletion of stratospheric ozone over recent decades, thus accelerating the need to protect human skin against the harmful effects of UV radiation such as erythema, edema, hyperpigmentation, photoaging, and skin cancer. There are many different ways to protect skin against UV radiation's harmful effects. The most popular way to reduce the amount of UV radiation penetrating the skin is topical application of sunscreen products that contain UV absorbing or reflecting active molecules. Based on their protection mechanism, the active molecules in sunscreens are broadly divided into inorganic and organic agents. Inorganic sunscreens reflect and scatter UV and visible radiation, while organic sunscreens absorb UV radiation and then re-emit energy as heat or light. These synthetic molecules have limited concentration according to regulation concern. Several natural compounds with UV absorption property have been used to substitute for or to reduce the quantity of synthetic sunscreen agents. In addition to UV absorption property, most natural compounds were found to act as antioxidants, anti-inflammatory, and immunomodulatory agents, which provide further protection against the damaging effects of UV radiation exposure. Compounds derived from natural sources have gained considerable attention for use in sunscreen products and have bolstered the market trend toward natural cosmetics. This adds to the importance of there being a wide selection of active molecules in sunscreen formulations. This paper summarizes a number of natural products derived from propolis, plants, algae, and lichens that have shown potential photoprotection properties against UV radiation exposure-induced skin damage. © 2015 Wiley Periodicals, Inc.

  11. Marine natural products.

    Science.gov (United States)

    Blunt, John W; Copp, Brent R; Keyzers, Robert A; Munro, Murray H G; Prinsep, Michèle R

    2016-03-01

    This review covers the literature published in 2014 for marine natural products (MNPs), with 1116 citations (753 for the period January to December 2014) referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms. The emphasis is on new compounds (1378 in 456 papers for 2014), together with the relevant biological activities, source organisms and country of origin. Reviews, biosynthetic studies, first syntheses, and syntheses that lead to the revision of structures or stereochemistries, have been included.

  12. Natural Product Molecular Fossils.

    Science.gov (United States)

    Falk, Heinz; Wolkenstein, Klaus

    The natural products synthesized by organisms that were living a long time ago gave rise to their molecular fossils. These can consist of either the original unchanged compounds or they may undergo peripheral transformations in which their skeletons remain intact. In cases when molecular fossils can be traced to their organismic source, they are termed "geological biomarkers".This contribution describes apolar and polar molecular fossils and, in particular biomarkers, along the lines usually followed in organic chemistry textbooks, and points to their bioprecursors when available. Thus, the apolar compounds are divided in linear and branched alkanes followed by alicyclic compounds and aromatic and heterocyclic molecules, and, in particular, the geoporphyrins. The polar molecular fossils contain as functional groups or constituent units ethers, alcohols, phenols, carbonyl groups, flavonoids, quinones, and acids, or are polymers like kerogen, amber, melanin, proteins, or nucleic acids. The final sections discuss the methodology used and the fundamental processes encountered by the biomolecules described, including diagenesis, catagenesis, and metagenesis.

  13. Combinatorial synthesis of natural products

    DEFF Research Database (Denmark)

    Nielsen, John

    2002-01-01

    Combinatorial syntheses allow production of compound libraries in an expeditious and organized manner immediately applicable for high-throughput screening. Natural products possess a pedigree to justify quality and appreciation in drug discovery and development. Currently, we are seeing a rapid...... increase in application of natural products in combinatorial chemistry and vice versa. The therapeutic areas of infectious disease and oncology still dominate but many new areas are emerging. Several complex natural products have now been synthesised by solid-phase methods and have created the foundation...... for preparation of combinatorial libraries. In other examples, natural products or intermediates have served as building blocks or scaffolds in the synthesis of complex natural products, bioactive analogues or designed hybrid molecules. Finally, structural motifs from the biologically active parent molecule have...

  14. Review of natural product databases.

    Science.gov (United States)

    Xie, Tao; Song, Sicheng; Li, Sijia; Ouyang, Liang; Xia, Lin; Huang, Jian

    2015-08-01

    Many natural products have pharmacological or biological activities that can be of therapeutic benefit in treating diseases, and are also an important source of inspiration for development of potential novel drugs. The past few decades have witnessed extensive study of natural products for their promising prospects in application of medicinal chemistry, molecular biology and pharmaceutical sciences. Natural product databases have provided systematic collection of information concerning natural products and their derivatives, including structure, source and mechanisms of action, which significantly support modern drug discovery. Currently, a considerable number of natural product databases, such as TCM Database@Taiwan, TCMID, CEMTDD, SuperToxic and SuperNatural, have been developed, providing data such as integrated medicinal herbs, ingredients, 2D/3D structures of the compounds, related target proteins, relevant diseases, and metabolic toxicity and more. We focus on an analytical overview of current natural product databases, and further discuss the good, bad or imperfection of current ones, in the hope of better integrating existing relevant outcomes, thus providing new routes for future drug discovery. © 2015 John Wiley & Sons Ltd.

  15. Natural products used for diabetes.

    Science.gov (United States)

    Shapiro, Karen; Gong, William C

    2002-01-01

    To review the efficacy and safety of natural products commonly used for diabetes. English and Spanish-language journals retrieved through a MEDLINE search of articles published between 1960 and December 2001 using these index terms: Opuntia, karela, gymnema, tecoma, alpha lipoic acid, thioctic acid, ginseng, panaxans, and diabetes. Natural products have long been used in traditional systems of medicine for diabetes. Products in common use include nopal (prickly pear cactus), fenu-greek, karela (bitter melon), gymnema, ginseng, tronadora, chromium, and alpha-lipoic acid. The popularity of these products varies among people of different ethnicities. Nopal is the most commonly used herbal hypoglycemic among persons of Mexican descent. Karela is more commonly used by persons from Asian countries. Some of these agents have gained universal appeal. For a select number of products, studies have revealed single or multiple mechanisms of action. For several of these, high soluble fiber content is a contributing factor. Based on the available evidence, several natural products in common use can lower blood glucose in patients with diabetes. Commonly used natural products often have a long history of traditional use, and pharmacists who have a stronger understanding of these products are better positioned to counsel patients on their appropriate use.

  16. Synthesis of Polycyclic Natural Products

    Energy Technology Data Exchange (ETDEWEB)

    Nguyen, Tuan Hoang [Iowa State Univ., Ames, IA (United States)

    2003-01-01

    With the continuous advancements in molecular biology and modern medicine, organic synthesis has become vital to the support and extension of those discoveries. The isolations of new natural products allow for the understanding of their biological activities and therapeutic value. Organic synthesis is employed to aid in the determination of the relationship between structure and function of these natural products. The development of synthetic methodologies in the course of total syntheses is imperative for the expansion of this highly interdisciplinary field of science. In addition to the practical applications of total syntheses, the structural complexity of natural products represents a worthwhile challenge in itself. The pursuit of concise and efficient syntheses of complex molecules is both gratifying and enjoyable.

  17. Natural products in chemical biology

    National Research Council Canada - National Science Library

    Civjan, Natanya

    2012-01-01

    ... as models and the possibilities of using them in medicine"-- "This book provides a fairly general overview of the unique features of the small molecules referred to as Natural Products, explores how this traditionally organic chemistry-based field was transformed by insights from genetics and biochemistry, and highlights some promising future directions"--

  18. Dental Application of Natural Products

    Directory of Open Access Journals (Sweden)

    Hiroshi Sakagami

    2018-02-01

    Full Text Available This review article summarizes the recent progress in dental applications of natural products. Catechin gel showed selective antimicrobial activity, whereas the alkaline extract of various plant species rich in lignin carbohydrate complex (LCC showed much higher antiviral activity than lower molecular weight polyphenols. Mouthwash with the alkaline extract of a plant classified as OTC effectively reduced halitosis. Unexpectedly, many polyphenolic compounds purified from the natural kingdom showed much lower tumor-specificity against human oral squamous cell lines as compared with antitumor agents, although they showed apoptosis-inducing activity. The alkaline extract of bamboo leaf, which exerted various common biological activities with LCC, showed osteogenic activity by stimulating differentiation toward osteoblasts while inhibiting differentiation toward osteoclasts. LCC enhanced the dectin-2 mRNA expression in macrophages, whereas glucan showed anti-osteoblastic action via dectin-1. These data suggest that natural products exert their biological activity by interacting with these molecules.

  19. Natural Products from Mangrove Actinomycetes

    Science.gov (United States)

    Xu, Dong-Bo; Ye, Wan-Wan; Han, Ying; Deng, Zi-Xin; Hong, Kui

    2014-01-01

    Mangroves are woody plants located in tropical and subtropical intertidal coastal regions. The mangrove ecosystem is becoming a hot spot for natural product discovery and bioactivity survey. Diverse mangrove actinomycetes as promising and productive sources are worth being explored and uncovered. At the time of writing, we report 73 novel compounds and 49 known compounds isolated from mangrove actinomycetes including alkaloids, benzene derivatives, cyclopentenone derivatives, dilactones, macrolides, 2-pyranones and sesquiterpenes. Attractive structures such as salinosporamides, xiamycins and novel indolocarbazoles are highlighted. Many exciting compounds have been proven as potential new antibiotics, antitumor and antiviral agents, anti-fibrotic agents and antioxidants. Furthermore, some of their biosynthetic pathways have also been revealed. This review is an attempt to consolidate and summarize the past and the latest studies on mangrove actinomycetes natural product discovery and to draw attention to their immense potential as novel and bioactive compounds for marine drugs discovery. PMID:24798926

  20. System of Natural Health Products

    Directory of Open Access Journals (Sweden)

    Angelica Paoletti

    2011-01-01

    Full Text Available Introduction. The safety of vitamin K antagonists (VKA use can be compromised by many popular herbal supplements taken by individuals. The literature reports that 30% of warfarin-treated patients self-medicates with herbs. Possible interactions represent an health risk. We aimed to identify all herbs-oral anticoagulants interactions collected in the Italian database of suspected adverse reactions to “natural health” products. Methods. The Italian database of spontaneous reports of suspected adverse reactions to natural products was analyzed to address herb-VKAs interactions. Results. From 2002 to 2009, we identified 12 reports with 7 cases of INR reduction in patients treated with warfarin (n=3 and acenocoumarol (n=4, and 5 cases of INR increase (all warfarin associated. It was reported 8 different herbal products as possibly interacting. Discussion. Our study confirms the risk of interactions, highlighting the difficulty to characterize them and their mechanisms and, finally, prevent their onset. The reported data underline the urgent need of healthcare providers being aware of the possible interaction between natural products and VKA, also because of the critical clinical conditions affecting patients. This is the first step to have the best approach to understand possible INR alterations linked to herb-VKA interaction and to rightly educate patients in treatment with VKA.

  1. Synthesis, β-glucuronidase inhibition and molecular docking studies of hybrid bisindole-thiosemicarbazides analogs.

    Science.gov (United States)

    Taha, Muhammad; Ismail, Nor Hadiani; Imran, Syahrul; Rahim, Fazal; Wadood, Abdul; Khan, Huma; Ullah, Hayat; Salar, Uzma; Khan, Khalid Mohammed

    2016-10-01

    Hybrid bisindole-thiosemicarbazides analogs (1-18) were synthesized and screened for β-glucuronidase activity. All compounds showed varied degree of β-glucuronidase inhibitory potential when compared with standard d-saccharic acid 1,4-lactone (IC50=48.4±1.25μM). Compounds 4, 7, 9, 6, 5, 12, 17 and 18 showed exceptional β-glucuronidase inhibition with IC50 values ranging from 0.1 to 5.7μM. Compounds 1, 3, 8, 16, 13, 2 and 14 also showed better activities than standard with IC50 values ranging from 7.12 to 15.0μM. The remaining compounds 10, 11, and 15 showed good inhibitory potential with IC50 values 33.2±0.75, 21.4±0.30 and 28.12±0.25μM respectively. Molecular docking studies were carried out to confirm the binding interaction of the compounds. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Isolation of microbial natural products.

    Science.gov (United States)

    Sterner, Olov

    2012-01-01

    In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products.

  3. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 1. Learning Organic Chemistry Through Natural Products Natural Products - A Kaleidoscopic View. N R Krishnaswamy. Series Article Volume 1 Issue 1 January 1996 pp 56-62 ...

  4. Hot springs and cool natural products.

    Science.gov (United States)

    Kwon, Ho Jeong; Lee, Choong Hwan; Osada, Hiroyuki; Yoshida, Minoru; Imoto, Masaya

    2008-08-01

    Natural products have played a unique role in providing new tools and insights in chemical biology. The tremendous value of natural products was highlighted by scientists from Korea and Japan at the 4(th) Korea-Japan Chemical Biology symposium.

  5. TEACHING 'NATURAL PRODUCT CHEMISTRY' IN TANZANIA

    African Journals Online (AJOL)

    Temechegn

    Explain the stages of structure elucidation and know some of the techniques used. • Understand the significance of natural products within drug discovery and the valuable knowledge gained from traditional medicine. Lecture Outline. Introduction to Natural Product Chemistry. • What are natural products? • The history of ...

  6. Dragmacidin G, a Bioactive Bis-Indole Alkaloid from a Deep-Water Sponge of the Genus Spongosorites

    Directory of Open Access Journals (Sweden)

    Amy E. Wright

    2017-01-01

    Full Text Available A deep-water sponge of the genus Spongosorites has yielded a bis-indole alkaloid which we have named dragmacidin G. Dragmacidin G was first reported by us in the patent literature and has recently been reported by Hitora et al. from a sponge of the genus Lipastrotheya. Dragmacidin G is the first in this series of compounds to have a pyrazine ring linking the two indole rings. It also has a rare N-(2-mercaptoethyl-guanidine side chain. Dragmacidin G shows a broad spectrum of biological activity including inhibition of methicillin-resistant Staphylococcus aureus, Mycobacterium tuberculosis, Plasmodium falciparum, and a panel of pancreatic cancer cell lines.

  7. Natural products and anti-inflammatory activity.

    Science.gov (United States)

    Yuan, Gaofeng; Wahlqvist, Mark L; He, Guoqing; Yang, Min; Li, Duo

    2006-01-01

    The aim of this review paper was to summarise some commonly available natural products and their anti-inflammatory activity. We have collected data from MEDLINE, Current Contents and scientific journals, which included 92 publications. There are numerous natural products detailed in this literature; however we have summarized a few of the most commonly available and potent ones. In this paper, the natural products with anti-inflammatory activity including curcumin, parthenolide, cucurbitacins, 1,8-cineole, pseudopterosins, lyprinol, bromelain, flavonoids, saponins, marine sponge natural products and Boswellia serrata gum resin were reviewed. Natural products play a significant role in human health in relation to the prevention and treatment of inflammatory conditions. Further studies are being conducted to investigate the mechanism of action, metabolism, safety and long term side effect of these natural products, as well as interactions between these natural products with food and drug components.

  8. Teaching 'natural product chemistry' in Tanzania | Buchanan ...

    African Journals Online (AJOL)

    Natural products 'historically' and 'today' have vast importance. This article describes the course 'Natural Product Chemistry', a new course in the 2011/2012 academic year in the Faculty of Natural and Applied Sciences at St. John's University of Tanzania. It reveals how the course has been applied to the African and ...

  9. Super Natural II--a database of natural products.

    Science.gov (United States)

    Banerjee, Priyanka; Erehman, Jevgeni; Gohlke, Björn-Oliver; Wilhelm, Thomas; Preissner, Robert; Dunkel, Mathias

    2015-01-01

    Natural products play a significant role in drug discovery and development. Many topological pharmacophore patterns are common between natural products and commercial drugs. A better understanding of the specific physicochemical and structural features of natural products is important for corresponding drug development. Several encyclopedias of natural compounds have been composed, but the information remains scattered or not freely available. The first version of the Supernatural database containing ∼ 50,000 compounds was published in 2006 to face these challenges. Here we present a new, updated and expanded version of natural product database, Super Natural II (http://bioinformatics.charite.de/supernatural), comprising ∼ 326,000 molecules. It provides all corresponding 2D structures, the most important structural and physicochemical properties, the predicted toxicity class for ∼ 170,000 compounds and the vendor information for the vast majority of compounds. The new version allows a template-based search for similar compounds as well as a search for compound names, vendors, specific physical properties or any substructures. Super Natural II also provides information about the pathways associated with synthesis and degradation of the natural products, as well as their mechanism of action with respect to structurally similar drugs and their target proteins. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  10. An Overview of Some Natural Products with Two A-Level Science Club Natural Products Experiments

    Science.gov (United States)

    Sosabowski, Michael Hal; Olivier, George W. J.; Jawad, Hala; Maatta, Sieja

    2017-01-01

    Natural products are ubiquitous in nature but do not form a large proportion of the A-level syllabuses in the UK. In this article we briefly discuss a small selection of natural products, focusing on alcohols, aldehydes and ketones, and alkaloids. We then outline two natural product experiments that are suitable for A-level chemistry clubs or…

  11. Natural Connections on Riemannian Product Manifolds

    OpenAIRE

    Gribacheva, Dobrinka

    2011-01-01

    A Riemannian almost product manifold with integrable almost product structure is called a Riemannian product manifold. In the present paper the natural connections on such manifolds are studied, i.e. the linear connections preserving the almost product structure and the Riemannian metric.

  12. Nigerian Journal of Natural Products and Medicine

    African Journals Online (AJOL)

    Nigerian Journal of Natural Products and Medicine is published by the Nigerian Society of Pharmacognosy, a non profit organisation established in 1982 dedicated to the promotion of Pharmacognosy, Natural Products and Traditional Medicine. It has a current circulation of about 500 to scientists in Nigeria and abroad.

  13. Stereoselective Halogenation in Natural Product Synthesis.

    Science.gov (United States)

    Chung, Won-jin; Vanderwal, Christopher D

    2016-03-24

    At last count, nearly 5000 halogenated natural products have been discovered. In approximately half of these compounds, the carbon atom to which the halogen is bound is sp(3) -hybridized; therefore, there are an enormous number of natural products for which stereocontrolled halogenation must be a critical component of any synthesis strategy. In this Review, we critically discuss the methods and strategies used for stereoselective introduction of halogen atoms in the context of natural product synthesis. Using the successes of the past, we also attempt to identify gaps in our synthesis technology that would aid the synthesis of halogenated natural products, as well as existing methods that have not yet seen application in complex molecule synthesis. The chemistry described herein demonstrates yet again how natural products continue to provide the inspiration for critical advances in chemical synthesis. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Monoterpene bisindole alkaloids, from the African medicinal plant Tabernaemontana elegans, induce apoptosis in HCT116 human colon carcinoma cells.

    Science.gov (United States)

    Mansoor, Tayyab A; Borralho, Pedro M; Dewanjee, Saikat; Mulhovo, Silva; Rodrigues, Cecília M P; Ferreira, Maria-José U

    2013-09-16

    Tabernaemontana elegans is a medicinal plant used in African traditional medicine to treat several ailments including cancer. The aims of the present study were to identify anti-cancer compounds, namely apoptosis inducers, from Tabernaemontana elegans, and hence to validate its usage in traditional medicine. Six alkaloids, including four monomeric indole (1-3, and 6) and two bisindole (4 and 5) alkaloids, were isolated from the methanolic extract of Tabernaemontana elegans roots. The structures of these compounds were characterized by 1D and 2D NMR spectroscopic and mass spectrometric data. Compounds 1-6 along with compound 7, previously isolated from the leaves of the same species, were evaluated for in vitro cytotoxicity against HCT116 human colon carcinoma cells by the MTS metabolism assay. The cytotoxicity of the most promising compounds was corroborated by Guava-ViaCount flow cytometry assays. Selected compounds were next studied for apoptosis induction activity in HCT116 cells, by evaluation of nuclear morphology following Hoechst staining, and by caspase-3 like activity assays. Among the tested compounds (1-7), the bisindole alkaloids tabernaelegantine C (4) and tabernaelegantinine B (5) were found to be cytotoxic to HCT116 cells at 20 µM, with compound 5 being more cytotoxic than the positive control 5-Fluorouracil (5-FU), at a similar dose. In fact, even at 0.5 µM, compound 5 was more potent than 5-FU. Compounds 4 and 5 induced characteristic patterns of apoptosis in HCT116 cancer cells including, cell shrinkage, condensation, fragmentation of the nucleus, blebbing of the plasma membrane and chromatin condensation. Further, general caspase-3-like activity was increased in cells exposed to compounds 4 and 5, corroborating the nuclear morphology evaluation assays. Bisindole alkaloids tabernaelegantine C (4) and tabernaelegantinine B (5) were characterized as potent apoptosis inducers in HCT116 human colon carcinoma cells and as possible lead/scaffolds for

  15. Natural gas production verification tests

    International Nuclear Information System (INIS)

    1992-02-01

    This Environmental Assessment (EA) has been prepared by the Department of Energy (DOE) in compliance with the requirements of the National Environmental Policy Act of 1969. The Department of Energy (DOE) proposes to fund, through a contract with Petroleum Consulting Services, Inc. of Canton, Ohio, the testing of the effectiveness of a non-water based hydraulic fracturing treatment to increase gas recovery from low-pressure, tight, fractured Devonian Shale formations. Although Devonian Shales are found in the Appalachian, Michigan, and Illinois Basins, testing will be done only in the dominant, historical five state area of established production. The objective of this proposed project is to assess the benefits of liquid carbon dioxide (CO 2 )/sand stimulations in the Devonian Shale. In addition, this project would evaluate the potential nondamaging (to the formation) properties of this unique fracturing treatment relative to the clogging or chocking of pores and fractures that act as gas flow paths to the wellbore in the target gas-producing zones of the formation. This liquid CO 2 /sand fracturing process is water-free and is expected to facilitate gas well cleanup, reduce the time required for post-stimulation cleanup, and result in improved production levels in a much shorter time than is currently experienced

  16. New Synthetic Methods for Hypericum Natural Products

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Insik [Iowa State Univ., Ames, IA (United States)

    2006-01-01

    Organic chemistry has served as a solid foundation for interdisciplinary research areas, such as molecular biology and medicinal chemistry. An understanding of the biological activities and structural elucidations of natural products can lead to the development of clinically valuable therapeutic options. The advancements of modern synthetic methodologies allow for more elaborate and concise natural product syntheses. The theme of this study centers on the synthesis of natural products with particularly challenging structures and interesting biological activities. The synthetic expertise developed here will be applicable to analog syntheses and to other research problems.

  17. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 2. Learning Organic Chemistry Through Natural Products Determination of Absolute Stereochemistry. N R Krishnaswamy. Series Article Volume 1 Issue 2 February 1996 pp 40-46 ...

  18. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 16; Issue 12. Learning Organic Chemistry Through Natural Products - Architectural Designs in Molecular Constructions. N R Krishnaswamy. Volume 16 Issue 12 December 2011 pp 1287-1293 ...

  19. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 10. Learning Organic Chemistry Through Natural Products Architectural Designs in Molecular Constructions. N R Krishnaswamy. Series Article Volume 1 Issue 10 October 1996 pp 37-43 ...

  20. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 7. Learning Organic Chemistry Through Natural engine Products - Structure and Biological Functions. N R Krishnaswamy. Series Article Volume 1 Issue 7 July 1996 pp 23-30 ...

  1. Discovery of Repellents from Natural Products

    Science.gov (United States)

    Natural products are an ideal source of chemicals for topical application to human skin, and can be a means of personal protection from the bites of mosquitoes and other arthropods. This report covers a diverse array of natural compounds, and includes descriptions of observed correlations between ch...

  2. Microbial production of natural raspbery ketone

    NARCIS (Netherlands)

    Beekwilder, M.J.; Meer, van der I.M.; Sibbesen, O.; Broekgaarden, M.; Qvist, I.; Mikkelsen, J.D.; Hall, R.D.

    2007-01-01

    Raspberry ketone is an important compound for the flavour industry. It is frequently used in products such as soft drinks, sweets, puddings and ice creams. The compound can be produced by organic synthesis. Demand for natural raspberry ketone is growing considerably. However, this product is

  3. Microbial production of natural raspberry ketone

    NARCIS (Netherlands)

    Beekwilder, Jules; van der Meer, Ingrid M.; Sibbesen, Ole; Broekgaarden, Mans; Qvist, Ingmar; Mikkelsen, Joern D.; Hall, Robert D.

    2007-01-01

    Raspberry ketone is an important compound for the flavour industry. It is frequently used in products such as soft drinks, sweets, puddings and ice creams. The compound can be produced by organic synthesis. Demand for "natural" raspberry ketone is growing considerably. However, this product is

  4. Natural Products in the Discovery of Agrochemicals.

    Science.gov (United States)

    Loiseleur, Olivier

    2017-12-01

    Natural products have a long history of being used as, or serving as inspiration for, novel crop protection agents. Many of the discoveries in agrochemical research in the last decades have their origin in a wide range of natural products from a variety of sources. In light of the continuing need for new tools to address an ever-changing array of fungal, weed and insect pests, new agricultural practices and evolving regulatory requirements, the needs for new agrochemical tools remains as critical as ever. In that respect, nature continues to be an important source for novel chemical structures and biological mechanisms to be applied for the development of pest control agents. Here we review several of the natural products and their derivatives which contributed to shape crop protection research in past and present.

  5. Natural product discovery: past, present, and future.

    Science.gov (United States)

    Katz, Leonard; Baltz, Richard H

    2016-03-01

    Microorganisms have provided abundant sources of natural products which have been developed as commercial products for human medicine, animal health, and plant crop protection. In the early years of natural product discovery from microorganisms (The Golden Age), new antibiotics were found with relative ease from low-throughput fermentation and whole cell screening methods. Later, molecular genetic and medicinal chemistry approaches were applied to modify and improve the activities of important chemical scaffolds, and more sophisticated screening methods were directed at target disease states. In the 1990s, the pharmaceutical industry moved to high-throughput screening of synthetic chemical libraries against many potential therapeutic targets, including new targets identified from the human genome sequencing project, largely to the exclusion of natural products, and discovery rates dropped dramatically. Nonetheless, natural products continued to provide key scaffolds for drug development. In the current millennium, it was discovered from genome sequencing that microbes with large genomes have the capacity to produce about ten times as many secondary metabolites as was previously recognized. Indeed, the most gifted actinomycetes have the capacity to produce around 30-50 secondary metabolites. With the precipitous drop in cost for genome sequencing, it is now feasible to sequence thousands of actinomycete genomes to identify the "biosynthetic dark matter" as sources for the discovery of new and novel secondary metabolites. Advances in bioinformatics, mass spectrometry, proteomics, transcriptomics, metabolomics and gene expression are driving the new field of microbial genome mining for applications in natural product discovery and development.

  6. Using Genomics for Natural Product Structure Elucidation.

    Science.gov (United States)

    Tietz, Jonathan I; Mitchell, Douglas A

    2016-01-01

    Natural products (NPs) are the most historically bountiful source of chemical matter for drug development-especially for anti-infectives. With insights gleaned from genome mining, interest in natural product discovery has been reinvigorated. An essential stage in NP discovery is structural elucidation, which sheds light not only on the chemical composition of a molecule but also its novelty, properties, and derivatization potential. The history of structure elucidation is replete with techniquebased revolutions: combustion analysis, crystallography, UV, IR, MS, and NMR have each provided game-changing advances; the latest such advance is genomics. All natural products have a genetic basis, and the ability to obtain and interpret genomic information for structure elucidation is increasingly available at low cost to non-specialists. In this review, we describe the value of genomics as a structural elucidation technique, especially from the perspective of the natural product chemist approaching an unknown metabolite. Herein we first introduce the databases and programs of interest to the natural products chemist, with an emphasis on those currently most suited for general usability. We describe strategies for linking observed natural product-linked phenotypes to their corresponding gene clusters. We then discuss techniques for extracting structural information from genes, illustrated with numerous case examples. We also provide an analysis of the biases and limitations of the field with recommendations for future development. Our overview is not only aimed at biologically-oriented researchers already at ease with bioinformatic techniques, but also, in particular, at natural product, organic, and/or medicinal chemists not previously familiar with genomic techniques.

  7. Natural product-based amyloid inhibitors.

    Science.gov (United States)

    Velander, Paul; Wu, Ling; Henderson, Frances; Zhang, Shijun; Bevan, David R; Xu, Bin

    2017-09-01

    Many chronic human diseases, including multiple neurodegenerative diseases, are associated with deleterious protein aggregates, also called protein amyloids. One common therapeutic strategy is to develop protein aggregation inhibitors that can slow down, prevent, or remodel toxic amyloids. Natural products are a major class of amyloid inhibitors, and several dozens of natural product-based amyloid inhibitors have been identified and characterized in recent years. These plant- or microorganism-extracted compounds have shown significant therapeutic potential from in vitro studies as well as in vivo animal tests. Despite the technical challenges of intrinsic disordered or partially unfolded amyloid proteins that are less amenable to characterizations by structural biology, a significant amount of research has been performed, yielding biochemical and pharmacological insights into how inhibitors function. This review aims to summarize recent progress in natural product-based amyloid inhibitors and to analyze their mechanisms of inhibition in vitro. Major classes of natural product inhibitors and how they were identified are described. Our analyses comprehensively address the molecular interactions between the inhibitors and relevant amyloidogenic proteins. These interactions are delineated at molecular and atomic levels, which include covalent, non-covalent, and metal-mediated mechanisms. In vivo animal studies and clinical trials have been summarized as an extension. To enhance natural product bioavailability in vivo, emerging work using nanocarriers for delivery has also been described. Finally, issues and challenges as well as future development of such inhibitors are envisioned. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Naturally Efficient Emitters: Luminescent Organometallic Complexes Derived from Natural Products

    Science.gov (United States)

    Zhang, Wen-Hua; Young, David J.

    2013-08-01

    Naturally occurring molecules offer intricate structures and functionality that are the basis of modern medicinal chemistry, but are under-represented in materials science. Herein, we review recent literature describing the use of abundant and relatively inexpensive, natural products for the synthesis of ligands for luminescent organometallic complexes used for organic light emitting diodes (OLEDs) and related technologies. These ligands are prepared from the renewable starting materials caffeine, camphor, pinene and cinchonine and, with the exception of caffeine, impart performance improvements to the emissive metal complexes and resulting OLED devices, with emission wavelengths that span the visible spectrum from blue to red. The advantages of these biologically-derived molecules include improved solution processibility and phase homogeneity, brighter luminescence, higher quantum efficiencies and lower turn-on voltages. While nature has evolved these carbon-skeletons for specific purposes, they also offer some intriguing benefits in materials science and technology.

  9. Nature tourism: a sustainable tourism product

    Directory of Open Access Journals (Sweden)

    Violante Martínez Quintana

    2017-11-01

    Full Text Available Nature tourism has emerged in the tourism field as a result of a logical evolution in line with public policies and academic research. After negative outcomes from traditional models first raised the alarm, the entire sector has tried to foster local development based on models of responsibility and sustainability. This article revises key concepts of nature – based tourism and shows new tendencies and the perception of cultural landscapes that are seen as tourism products. Finally, it concludes by analysing new tendencies to foster alternative nature – based tourism. It also presents a planning proposal based on a responsible and sustainable tourism model to guarantee a sustainable tourism product within the natural and cultural heritage context.

  10. Psychoactive natural products: overview of recent developments

    Directory of Open Access Journals (Sweden)

    István Ujváry

    2014-03-01

    Full Text Available Natural psychoactive substances have fascinated the curious mind of shamans, artists, scholars and laymen since antiquity. During the twentieth century, the chemical composition of the most important psychoactive drugs, that is opium, cannabis, coca and "magic mushrooms", has been fully elucidated. The mode of action of the principal ingredients has also been deciphered at the molecular level. In the past two decades, the use of herbal drugs, such as kava, kratom and Salvia divinorum, began to spread beyond their traditional geographical and cultural boundaries. The aim of the present paper is to briefly summarize recent findings on the psychopharmacology of the most prominent psychoactive natural products. Current knowledge on a few lesser-known drugs, including bufotenine, glaucine, kava, betel, pituri, lettuce opium and kanna is also reviewed. In addition, selected cases of alleged natural (or semi-natural products are also mentioned.

  11. Countercurrent Separation of Natural Products: An Update

    Science.gov (United States)

    2015-01-01

    This work assesses the current instrumentation, method development, and applications in countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), collectively referred to as countercurrent separation (CCS). The article provides a critical review of the CCS literature from 2007 since our last review (J. Nat. Prod.2008, 71, 1489–1508), with a special emphasis on the applications of CCS in natural products research. The current state of CCS is reviewed in regard to three continuing topics (instrumentation, solvent system development, theory) and three new topics (optimization of parameters, workflow, bioactivity applications). The goals of this review are to deliver the necessary background with references for an up-to-date perspective of CCS, to point out its potential for the natural product scientist, and thereby to induce new applications in natural product chemistry, metabolome, and drug discovery research involving organisms from terrestrial and marine sources. PMID:26177360

  12. Chemoselective enrichment for natural products discovery.

    Science.gov (United States)

    Odendaal, Antoinette Y; Trader, Darci J; Carlson, Erin E

    2011-04-01

    Natural products account for a significant proportion of modern day therapeutic agents. However, the discovery of novel compounds is hindered by the isolation process, which often relies upon extraction and chromatographic separation techniques. These methods, which are dependent upon the physicochemical properties of the compounds, have a limited ability to both purify and concentrate the minor components of a biological extract. We have devised an isolation strategy based upon an orthogonal chemical feature, namely, functional group composition. Development of a functional group-targeted method is expected to achieve exceptional resolution given the large number of distinct moieties present in natural product extracts. Here, we describe the generation of controllably reversible covalent enrichment tags for the chemoselective isolation of alcohol-containing natural products from complex mixtures.

  13. Antiviral Natural Products and Herbal Medicines

    Directory of Open Access Journals (Sweden)

    Liang-Tzung Lin

    2014-01-01

    Full Text Available Viral infections play an important role in human diseases, and recent outbreaks in the advent of globalization and ease of travel have underscored their prevention as a critical issue in safeguarding public health. Despite the progress made in immunization and drug development, many viruses lack preventive vaccines and efficient antiviral therapies, which are often beset by the generation of viral escape mutants. Thus, identifying novel antiviral drugs is of critical importance and natural products are an excellent source for such discoveries. In this mini-review, we summarize the antiviral effects reported for several natural products and herbal medicines.

  14. Natural antioxidants in meat and poultry products.

    Science.gov (United States)

    Karre, Liz; Lopez, Keyla; Getty, Kelly J K

    2013-06-01

    In response to recent claims that synthetic antioxidants have the potential to cause toxicological effects and consumers' increased interest in purchasing natural products, the meat and poultry industry has been seeking sources of natural antioxidants. Due to their high phenolic compound content, fruits and other plant materials provide a good alternative to conventional antioxidants. Plum, grape seed extract, cranberry, pomegranate, bearberry, pine bark extract, rosemary, oregano, and other spices functions as antioxidants in meat and poultry products. Pomegranate, pine bark extract, cinnamon, and cloves have exhibited stronger antioxidant properties than some synthetic options. Plum products, grape seed extract, pine bark extract, rosemary, and some spices all have been shown to affect the color of finished meat or poultry products; however, in some products such as pork sausage or uncured meats, an increase in red color may be desired. When selecting a natural antioxidant, sensory and quality impact on the product should be considered to achieve desired traits. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Natural products – learning chemistry from plants

    NARCIS (Netherlands)

    Staniek, A.; Bouwmeester, H.J.; Fraser, P.D.; Kayser, O.; Martens, S.; Tissier, A.; Krol, van der A.R.; Wessjohann, L.; Warzecha, H.

    2014-01-01

    Plant natural products (PNPs) are unique in that they represent a vast array of different structural features, ranging from relatively simple molecules to very complex ones. Given the fact that many plant secondary metabolites exhibit profound biological activity, they are frequently used as

  16. Plants as natural antioxidants for meat products

    Science.gov (United States)

    Tomović, V.; Jokanović, M.; Šojić, B.; Škaljac, S.; Ivić, M.

    2017-09-01

    The meat industry is demanding antioxidants from natural sources to replace synthetic antioxidants because of the negative health consequences or beliefs regarding some synthetic ones. Plants materials provide good alternatives. Spices and herbs, generally used for their flavouring characteristics, can be added to meat products in various forms: whole, ground, or as isolates from their extracts. These natural antioxidants contain some active compounds, which exert antioxidative potential in meat products. This antioxidant activity is most often due to phenolic acids, phenolic diterpenes, flavonoids and volatile oils. Each of these compounds often has strong H-donating activity, thus making them extremely effective antioxidants; some compounds can chelate metals and donate H to oxygen radicals, thus slowing oxidation via two mechanisms. Numerous studies have demonstrated the efficacy of natural antioxidants when used in meat products. Based on this literature review, it can be concluded that natural antioxidants are added to fresh and processed meat and meat products to delay, retard, or prevent lipid oxidation, retard development of off-flavours (rancidity), improve colour stability, improve microbiological quality and extend shelf-life, without any damage to the sensory or nutritional properties.

  17. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 9. Learning Organic Chemistry Through Natural Products A Practical Approach. N R Krishnaswamy. Series Article Volume 1 Issue 9 September 1996 pp 25-33. Fulltext. Click here to view fulltext PDF. Permanent link:

  18. Computational approaches to natural product discovery

    NARCIS (Netherlands)

    Medema, M.H.; Fischbach, M.A.

    2015-01-01

    Starting with the earliest Streptomyces genome sequences, the promise of natural product genome mining has been captivating: genomics and bioinformatics would transform compound discovery from an ad hoc pursuit to a high-throughput endeavor. Until recently, however, genome mining has advanced

  19. Chocolate: A Marvelous Natural Product of Chemistry

    Science.gov (United States)

    Tannenbaum, Ginger

    2004-01-01

    The study of chocolate, a natural product, can be beneficial for the chemistry students as they ask frequently about the relevancy of their chemistry classes. The history of chocolate, its chemical and physical changes during processing, its composition, different crystalline forms, tempering and its viscosity are discussed.

  20. Standardization for natural product synthetic biology

    NARCIS (Netherlands)

    Zhao, Huimin; Medema, Marnix H.

    2016-01-01

    Standardization is one of the foundational features of modern-day engineering, and the use of standardized parts and processes is a key element that distinguishes bona fide synthetic biology from traditional genetic engineering. Here, we discuss the role of standardization in natural product

  1. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 5. Learning Organic Chemistry Through Natural Products From Molecular and Electronic Structures to Reactivity. N R Krishnaswamy. Series Article Volume 1 Issue 5 May 1996 pp 12-18 ...

  2. Marine Natural Products as Prototype Agrochemical Agents

    Science.gov (United States)

    Peng, Jiangnan; Shen, Xiaoyu; El Sayed, Khalid A.; Dunbar, D. C Harles; Perry, Tony L.; Wilkins, Scott P.; Hamann, Mark T.; Bobzin, Steve; Huesing, Joseph; Camp, Robin; Prinsen, Mike; Krupa, Dan; Wideman, Margaret A.

    2016-01-01

    In the interest of identifying new leads that could serve as prototype agrochemical agents, 18 structurally diverse marine-derived compounds were examined for insecticidal, herbicidal, and fungicidal activities. Several new classes of compounds have been shown to be insecticidal, herbicidal, and fungicidal, which suggests that marine natural products represent an intriguing source for the discovery of new agrochemical agents. PMID:12670165

  3. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 1; Issue 2. Learning Organic Chemistry Through Natural Products Determination of Absolute ... Author Affiliations. N R Krishnaswamy1. Visiting Professor, NMKRV College for Women with PG Centre, Jayanagar III Block, Bangalore 560011, India.

  4. Natural products from microbes associated with insects

    DEFF Research Database (Denmark)

    Beemelmanns, Christine; Guo, Huijuan; Rischer, Maja

    2016-01-01

    Here we review discoveries of secondary metabolites from microbes associated with insects. We mainly focus on natural products, where the ecological role has been at least partially elucidated, and/or the pharmaceutical properties evaluated, and on compounds with unique structural features. We...

  5. Synthesis of natural products of therapeutic significance

    Indian Academy of Sciences (India)

    system

    2015-11-07

    Nov 7, 2015 ... animals or microorganisms. <10% of the world bio-diversity has been evaluated for the potential activity, while more natural products await the evaluation. Generally derived from countless bio-synthetic pathways and numerous reactions and mechanisms involving the building blocks such as acetyl ...

  6. Learning Organic Chemistry Through Natural Products -12 ...

    Indian Academy of Sciences (India)

    N R Krishnaswamy was initiated into the world of natural products by T R Seshadri at. University of Delhi and has carried on the glorious traditions of his mentor. He has taught at Banga- lore University, Calicut. University and. Sri Sathya Sai Institute of. Higher Learning. Generations of students would vouch for the fact.

  7. The Rakicidin Family of Anticancer Natural Products

    DEFF Research Database (Denmark)

    Tsakos, Michail; Jacobsen, Kristian Mark; Yu, Wanwan

    2016-01-01

    Rakicidin A is a prominent member of a small class of macrocyclic lipodepsipeptide natural products that contain an electrophilic 4- amido-2,4-pentadienoate (APD) functionality. Rakicidin A displays selective growth inhibitory activity against hypoxic cancer cells as well as imatinib...

  8. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    However, geraniol lacks an important feature present in many organic compounds, in general, and in natural products, in particular. The molecular structure of geraniol is superimposable on its mirror image and hence it is achiral. The problem of structure determination has an added dimension in molecules which are chiral.

  9. Environmental Impact of Natural Gas Hydrate Production

    Science.gov (United States)

    Max, M. D.; Johnson, A. H.

    2017-12-01

    Unmet conventional energy demand is encouraging a number of deep energy importing nations closer to production of their potentially very large Natural Gas Hydrate (NGH) resources. As methane and other natural gases are potent greenhouse gases, concerns exist about the possible environmental risks associated NGH development. Accidental of natural gas would have environmental consequences. However, the special characteristics of NGH and production models indicate a very low environmental risk from the reservoir to the deepwater wellhead that is much lower than for conventional deepwater gas. NGH is naturally stable in its solid form in the reservoir and shutting in the gas can be achieved by stopping NGH conversion and gas production in the reservoir. Rapid shut down results in re-crystallization of gas and stabilization of the reservoir through NGH reformation. In addition, new options for innovative technologies have the potential to allow safe development of NGH at a fraction of the current estimated cost. Gas produced from NGH is about the same as processed conventional gas, although almost certainly more pure. Leakage of gas during transport is not a production issue. Gas transport leakage is a matter for best practices regulation that is rigorously enforced.

  10. Learning Organic Chemistry Through Natural Products -12 ...

    Indian Academy of Sciences (India)

    The leaves of this plant are tradi- tionally used for the treatment of jaundice and other liver dis- orders. like T R Seshadri and K. Venkataraman, T R Govinda- chari has also made significant contributions to the chemistry of natural products. He had his initial training from the Ameri- can chemist Roger Adams. Intramolecular.

  11. Natural products in modern life science

    Science.gov (United States)

    Göransson, Ulf; Alsmark, Cecilia; Wedén, Christina; Backlund, Anders

    2010-01-01

    With a realistic threat against biodiversity in rain forests and in the sea, a sustainable use of natural products is becoming more and more important. Basic research directed against different organisms in Nature could reveal unexpected insights into fundamental biological mechanisms but also new pharmaceutical or biotechnological possibilities of more immediate use. Many different strategies have been used prospecting the biodiversity of Earth in the search for novel structure–activity relationships, which has resulted in important discoveries in drug development. However, we believe that the development of multidisciplinary incentives will be necessary for a future successful exploration of Nature. With this aim, one way would be a modernization and renewal of a venerable proven interdisciplinary science, Pharmacognosy, which represents an integrated way of studying biological systems. This has been demonstrated based on an explanatory model where the different parts of the model are explained by our ongoing research. Anti-inflammatory natural products have been discovered based on ethnopharmacological observations, marine sponges in cold water have resulted in substances with ecological impact, combinatory strategy of ecology and chemistry has revealed new insights into the biodiversity of fungi, in depth studies of cyclic peptides (cyclotides) has created new possibilities for engineering of bioactive peptides, development of new strategies using phylogeny and chemography has resulted in new possibilities for navigating chemical and biological space, and using bioinformatic tools for understanding of lateral gene transfer could provide potential drug targets. A multidisciplinary subject like Pharmacognosy, one of several scientific disciplines bridging biology and chemistry with medicine, has a strategic position for studies of complex scientific questions based on observations in Nature. Furthermore, natural product research based on intriguing scientific

  12. Automated genome mining for natural products

    Directory of Open Access Journals (Sweden)

    Zajkowski James

    2009-06-01

    Full Text Available Abstract Background Discovery of new medicinal agents from natural sources has largely been an adventitious process based on screening of plant and microbial extracts combined with bioassay-guided identification and natural product structure elucidation. Increasingly rapid and more cost-effective genome sequencing technologies coupled with advanced computational power have converged to transform this trend toward a more rational and predictive pursuit. Results We have developed a rapid method of scanning genome sequences for multiple polyketide, nonribosomal peptide, and mixed combination natural products with output in a text format that can be readily converted to two and three dimensional structures using conventional software. Our open-source and web-based program can assemble various small molecules composed of twenty standard amino acids and twenty two other chain-elongation intermediates used in nonribosomal peptide systems, and four acyl-CoA extender units incorporated into polyketides by reading a hidden Markov model of DNA. This process evaluates and selects the substrate specificities along the assembly line of nonribosomal synthetases and modular polyketide synthases. Conclusion Using this approach we have predicted the structures of natural products from a diverse range of bacteria based on a limited number of signature sequences. In accelerating direct DNA to metabolomic analysis, this method bridges the interface between chemists and biologists and enables rapid scanning for compounds with potential therapeutic value.

  13. In silico Design of "Un-Natural" Natural Products

    Directory of Open Access Journals (Sweden)

    Zucko; J. ...(et al.

    2008-05-01

    Full Text Available Polyketides and non-ribosomal peptides represent a large class of structurally diverse natural products much studied over recent years because the enzymes that synthesise them, the modular polyketide synthases (PKSs and the non-ribosomal peptide synthetases (NRPSs, share striking architectural similarities that can be exploited to generate "un-natural" natural products. PKS and NRPS proteins are multifunctional, composed of a co-linear arrangement of discrete protein domains representing each enzymic activity needed for chain elongation using either carboxylic acid or amino acid building blocks. Each domain is housed within larger modules which form the complex. Polyketide and peptide antibiotics, antifungals, antivirals, cytostatics, immunosuppressants, antihypertensives, antidiabetics, antimalarials and anticholesterolemics are in clinical use. Of commercial importance are also polyketide and peptide antiparasitics, coccidiostatics,animal growth promoters and natural insecticides.Polyketides are assembled through serial condensations of activated coenzyme-A thioester monomers derived from simple organic acids such as acetate, propionate and butyrate. The choice of organic acid allows the introduction of different chiral centres into the polyketide backbone. The active sites required for condensation include an acyltransferase (AT, an acyl carrier protein (ACP and a ß-ketoacylsynthase (KS. Each condensation results in a ß-keto group that undergoes all, some or none of a series of processing steps. Active sites that perform these reactions are contained within the following domains; ketoreductase (KR, dehydratase (DH and an enoylreductase (ER. The absence of any ß-keto processing results in the incorporation of a ketone group into the growing polyketide chain, a KR alone gives rise to a hydroxyl moiety, a KR and DH produce an alkene, while the combination of KR, DH and ER domains lead to complete reduction to an alkane. Most often, the last

  14. Utilization of Natural Products as Functional Feed

    Directory of Open Access Journals (Sweden)

    Stella Magdalena

    2013-03-01

    Full Text Available The use of antibiotics as feed additive improves performance in livestock. However, scientific data related to the use of antibiotics in feed merge spreading of bacterial resistance in animal and human bodies, therefore the usage of antibiotics in animal production is restricted. This condition raise the utilization of natural antibiotic as functional feed such as phytogenics (essential oil, flavonoid, saponin, and tannin, enzyme, probiotic, and prebiotic to improve the livestock’s performance, quality, and health. Functional feeds increase profitability in animal husbandry production and its use is feeds are expected to be functional foods that may have positive effects in human nutrition.

  15. (+-Discodermolide: A Marine Natural Product Against Cancer

    Directory of Open Access Journals (Sweden)

    Marcus Viníus Nora De Souza

    2004-01-01

    Full Text Available (+-Discodermolide was isolated in 1990 by Gunasekera et al. from the deep-water Caribbean sponge Discodermia dissoluta. It attacks cancer cells in a similar way to the successful cancer drug Taxol® that has become the best-selling anticancer drug in history. Taxol is also the first natural product described that stabilizes the microtubules involved in many aspects of cellular biology and that represent an important target of anticancer chemotherapeutics. However, (+-discodermolide appears to be far more potent than Taxol® against tumors that have developed multiple-drug resistance, with an IC50 in the low nanomolar range. Due to these excellent results, this natural product was licensed to Novartis Pharmaceutical Corporation in early 1998. The present review covers the history, biological activity, total synthesis, and synthetic analogs of (+-discodermolide.

  16. New chemistry from natural product biosynthesis.

    Science.gov (United States)

    Hubert, Catherine B; Barry, Sarah M

    2016-06-15

    Catalysts are a vital part of synthetic chemistry. However, there are still many important reactions for which catalysts have not been developed. The use of enzymes as biocatalysts for synthetic chemistry is growing in importance due to the drive towards sustainable methods for producing both bulk chemicals and high value compounds such as pharmaceuticals, and due to the ability of enzymes to catalyse chemical reactions with excellent stereoselectivity and regioselectivity. Such challenging transformations are a common feature of natural product biosynthetic pathways. In this mini-review, we discuss the potential to use biosynthetic pathways as a starting point for biocatalyst discovery. We introduce the reader to natural product assembly and tailoring, then focus on four classes of enzyme that catalyse C─H bond activation reactions to functionalize biosynthetic precursors. Finally, we briefly discuss the challenges involved in novel enzyme discovery. © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

  17. Trypanocidal Activity of Marine Natural Products

    Directory of Open Access Journals (Sweden)

    Amy J. Jones

    2013-10-01

    Full Text Available Marine natural products are a diverse, unique collection of compounds with immense therapeutic potential. This has resulted in these molecules being evaluated for a number of different disease indications including the neglected protozoan diseases, human African trypanosomiasis and Chagas disease, for which very few drugs are currently available. This article will review the marine natural products for which activity against the kinetoplastid parasites; Trypanosoma brucei brucei, T.b. rhodesiense and T. cruzi has been reported. As it is important to know the selectivity of a compound when evaluating its trypanocidal activity, this article will only cover molecules which have simultaneously been tested for cytotoxicity against a mammalian cell line. Compounds have been grouped according to their chemical structure and representative examples from each class were selected for detailed discussion.

  18. Antifungal potential of marine natural products

    OpenAIRE

    El-Hossary, Ebaa M.; Cheng, Cheng; Hamed, Mostafa M.; El-Sayed Hamed, Ashraf Nageeb; Ohlsen, Knut; Hentschel, Ute; Abdelmohsen, Usama Ramadan

    2017-01-01

    Highlights: • Fungal infections represent an increasing threat to human health. • Fungal infections in plants are a worldwide problem to the agricultural industry. • Diverse antifungal compounds were isolated from different marine organisms. • The number of new antifungal marine natural products is rapidly developing. • Marine sponges and bacteria are the predominant sources for antifungal compounds. Abstract: Fungal diseases represent an increasing threat to human healt...

  19. Biotechnological potential of marine natural products

    OpenAIRE

    Fusetani, Nobuhiro

    2010-01-01

    The number of marine natural products (MNPs) that have been applied to biotechnological industry is very limited, although nearly 20000 new compounds were discovered from marine organisms since the birth of MNPs in the early 1970s. However, it is apparent that they have a significant potential as pharmaceuticals, cosmetics, nutraceuticals, research tools, and others. This article focuses on selective antitumor metabolites isolated from marine sponges and tunicates, and their modes of action, ...

  20. Natural Products Combating Neurodegeneration: Parkinson's Disease.

    Science.gov (United States)

    Solayman, Md; Islam, Md Asiful; Alam, Fahmida; Khalil, Md Ibrahim; Kamal, Mohammad Amjad; Gan, Siew Hua

    2017-01-01

    Parkinson's disease (PD) is characterized by neurodegeneration and a progressive functional impairment of the midbrain nigral dopaminergic neurons. The cause remains unknown; however, several pathological processes and central factors, such as protein aggregation, mitochondrial dysfunction, iron accumulation, neuroinflammation and oxidative stress, have been reported. The current treatment method primarily targets symptoms by using anti-Parkinson drugs such as levodopa, carbidopa, dopamine (DA) agonists, monoamine oxidase type B inhibitors and anticholinergics to replace DA. When drug therapy is not satisfactory, surgical treatments are recommended. Unfortunately, the existing conventional strategies that target PD are associated with numerous side effects and possess an economic burden. Therefore, novel therapeutic approaches that regulate the pathways leading to neuronal death and dysfunction are necessary. For many years, nature has provided the primary resource for the discovery of potential therapeutic agents. Remarkably, many natural products from medicinal plants, fruits and vegetables have been demonstrated to be efficacious anti-Parkinson agents. These products possess neuroprotective properties as a result of not only their wellrecognized anti-oxidative and anti-inflammatory activities but also their inhibitory roles regarding iron accumulation, protein misfolding and the maintenance of proteasomal degradation, as well as mitochondrial homeostasis. The aim of this review is to report the available anti-Parkinson agents based on natural products and delineate their therapeutic actions, which act on various pathways. Overall, this review emphasizes the types of natural products that are potential future resources in the treatment of PD as novel regimens or supplementary agents. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  1. Natural cold pressed oils as cosmetic products

    Directory of Open Access Journals (Sweden)

    Małgorzata Ligęza

    2016-12-01

    Full Text Available Background. It seems that patients may ask general practitioners about natural cosmetics applied on the skin regarding their safety and suitability. Objectives. The aim of the study was to analyze natural cold pressed oils as potential cosmetic products. Material and methods. Cold pressed oils obtained from selected seeds and fruit stones were analyzed, including: chokeberry seed oil, blackcurrant seed oil, elderberry seed oil, raspberry seed oil, apricot seed oil, tomato seed oil, strawberry seed oil, broccoli seed oil, Nigella sativa seed oil, hemp oil, safflower seed oil, Silybum marianum seed oil and coconut oil. 80 adult volunteers assessed the cosmetic properties of the analyzed oils. Each of the volunteers tested 2 to 4 different oils, by applying them on the skin. In addition, patch tests with all analyzed oils were performed on 23 individuals. Results. The majority of tested oils were positively evaluated by the participants: in the opinion of the participants, oil extracted from safflower had the best appearance (100% positive opinions, coconut oil had the best smell (70% positive opinions, while black currant seed oil showed the best absorbency (85% positive opinions. No irritation was observed within the analyzed product group, albeit one allergic reaction to apricot seed oil was observed with patch testing. Conclusions . Based on the achieved results, it could be suggested that natural cold pressed oils can be applied to the skin as cosmetics. Our observations may be helpful for general practitioners when choosing natural cosmetics.

  2. Natural and Heterologous Production of Bacteriocins

    Science.gov (United States)

    Cintas, Luis M.; Herranz, Carmen; Hernández, Pablo E.

    Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria, and their use as natural and nontoxic food preservatives has been the source of considerable interest for the research community. In addition, bacteriocins have been investigated for their potential use in human and veterinary applications and in the animal production field. In the native bacterial strain, most bacteriocins are synthesized as biologically inactive precursors, with N-terminal extensions, that are cleaved concomitantly during export of the bacteriocin by dedicated ABC transporters, or the general secretory pathway (GSP) or Sec-dependent pathway. However, a few bacteriocins are synthesized without an N-terminal extension, and others are circularized through a head-to-tail peptide bond, complicating the elucidation of their processing and transport across the cytoplasmic membrane. The high cost of synthetic bacteriocin synthesis and their low yields from many natural producers recommends the exploration of recombinant microbial systems for the heterologous production of bacteriocins. Other advantages of such systems include production of bacteriocins in safer hosts, increased bacteriocin production, control of bacteriocin gene expression, production of food ingredients with antimicrobial activity, construction of multibacteriocinogenic strains with a wider antagonistic spectrum, a better adaptation of the selected hosts to food environments, and providing antagonistic properties to lactic acid bacteria (LAB) used as starter, protective, or probiotic cultures. The recombinant production of bacteriocins mostly relies on the use of expression vectors that replicate in Gram-negative bacteria, Gram-positive bacteria, and yeasts, whereas the production of bacteriocins in heterologous LAB hosts may be essentially based on the expression of native biosynthetic genes, by exchanging or replacing leader peptides and/or dedicated processing and secretion systems (ABC transporters

  3. Chitin elicitation of natural product production in marine bacteria

    DEFF Research Database (Denmark)

    Månsson, Maria; Wietz, Matthias; Larsen, Thomas Ostenfeld

    Genome sequences reveal that our current standard laboratory conditions only support a fraction of the potential secondary metabolism in bacteria.1 Thus, we must rethink cultivation, detection, and isolation strategies for bacterial secondary metabolites in order to explore the huge, so far...... on glucose-based medium. The different phenotypic responses to a natural growth substrate may reflect different niche-adaptations or ecological functions of the compounds produced and it represents a fruitful approach for elicitation of natural product production in marine bacteria....... uncharacterized chemical potential of these organisms. As part of a new project on ecology-driven drug discovery at the Technical University of Denmark, we investigate the use of chitin to elicit or alter production of antibacterial compounds in marine bacteria. Within our large collection of Gram...

  4. Natural-Product-Derived Carbon Dots: From Natural Products to Functional Materials.

    Science.gov (United States)

    Zhang, Xinyue; Jiang, Mingyue; Niu, Na; Chen, Zhijun; Li, Shujun; Liu, Shouxin; Li, Jian

    2018-01-10

    Nature provides an almost limitless supply of sources that inspire scientists to develop new materials with novel applications and less of an environmental impact. Recently, much attention has been focused on preparing natural-product-derived carbon dots (NCDs), because natural products have several advantages. First, natural products are renewable and have good biocompatibility. Second, natural products contain heteroatoms, which facilitate the fabrication of heteroatom-doped NCDs without the addition of an external heteroatom source. Finally, some natural products can be used to prepare NCDs in ways that are very green and simple relative to traditional methods for the preparation of carbon dots from man-made carbon sources. NCDs have shown tremendous potential in many fields, including biosensing, bioimaging, optoelectronics, and photocatalysis. This Review addresses recent progress in the synthesis, properties, and applications of NCDs. The challenges and future direction of research on NCD-based materials in this booming field are also discussed. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Dithiolopyrrolone Natural Products: Isolation, Synthesis and Biosynthesis

    Science.gov (United States)

    Qin, Zhiwei; Huang, Sheng; Yu, Yi; Deng, Hai

    2013-01-01

    Dithiolopyrrolones are a class of antibiotics that possess the unique pyrrolinonodithiole (4H-[1,2] dithiolo [4,3-b] pyrrol-5-one) skeleton linked to two variable acyl groups. To date, there are approximately 30 naturally occurring dithiolopyrrolone compounds, including holomycin, thiolutin, and aureothricin, and more recently thiomarinols, a unique class of hybrid marine bacterial natural products containing a dithiolopyrrolone framework linked by an amide bridge with an 8-hydroxyoctanoyl chain linked to a monic acid. Generally, dithiolopyrrolone antibiotics have broad-spectrum antibacterial activity against various microorganisms, including Gram-positive and Gram-negative bacteria, and even parasites. Holomycin appeared to be active against rifamycin-resistant bacteria and also inhibit the growth of the clinical pathogen methicillin-resistant Staphylococcus aureus N315. Its mode of action is believed to inhibit RNA synthesis although the exact mechanism has yet to be established in vitro. A recent work demonstrated that the fish pathogen Yersinia ruckeri employs an RNA methyltransferase for self-resistance during the holomycin production. Moreover, some dithiolopyrrolone derivatives have demonstrated promising antitumor activities. The biosynthetic gene clusters of holomycin have recently been identified in S. clavuligerus and characterized biochemically and genetically. The biosynthetic gene cluster of thiomarinol was also identified from the marine bacterium Pseudoalteromonas sp. SANK 73390, which was uniquely encoded by two independent pathways for pseudomonic acid and pyrrothine in a novel plasmid. The aim of this review is to give an overview about the isolations, characterizations, synthesis, biosynthesis, bioactivities and mode of action of this unique family of dithiolopyrrolone natural products, focusing on the period from 1940s until now. PMID:24141227

  6. Appreciation of symmetry in natural product synthesis.

    Science.gov (United States)

    Bai, Wen-Ju; Wang, Xiqing

    2017-12-13

    Covering: 2012 to June 2017This review aims to show that complex natural product synthesis can be streamlined by taking advantage of molecular symmetry. Various strategies to construct molecules with either evident or hidden symmetry are illustrated. Insights regarding the origins and adjustments of these strategies as well as inspiring new methodological developments are deliberated. When a symmetric strategy fails, the corresponding reason is analysed and an alternative approach is briefly provided. Finally, the importance of exploiting molecular symmetry and future research directions are discussed.

  7. Chemical Biology of Microbial Anticancer Natural Products

    DEFF Research Database (Denmark)

    Bladt, Tanja Thorskov; Gotfredsen, Charlotte Held

    than 100 years. New natural products (NPs) are continually discovered and with the increase in selective biological assays, previously described compounds often also display novel bioactivities, justifying their presence in novel screening efforts. Screening and discovery of compounds with activity...... by cyanobacteria were discovered through target-guided isolation based on NMR. The micropeptins displayed inhibitory activity towards serine proteases: chymotrypsin and elastase with IC50 values between 5.9 and 28.0 μM. In conclusion, this PhD study adds to the knowledge of bioactive NPs produced by filamentous...

  8. Multimodular biocatalysts for natural product assembly

    Science.gov (United States)

    Schwarzer, Dirk; Marahiel, Mohamed A.

    2001-03-01

    Nonribosomal peptides and polyketides represent a large class of natural products that show an extreme structural diversity and broad pharmacological relevance. They are synthesized from simple building blocks such as amino or carboxy acids and malonate derivatives on multimodular enzymes called nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), respectively. Although utilizing different substrates, NRPSs and PKSs show striking similarities in the modular architecture of their catalytic domains and product assembly-line mechanism. Among these compounds are well known antibiotics (penicillin, vancomycin and erythromycin) as well as potent immunosuppressive agents (cyclosporin, rapamycin and FK 506). This review focuses on the modular organization of NRPSs, PKSs and mixed NRPS/PKS systems and how modules and domains that build up the biosynthetic templates can be exploited for the rational design of recombinant enzymes capable of synthesizing novel compounds.

  9. Synthetic biology of fungal natural products

    Science.gov (United States)

    Mattern, Derek J.; Valiante, Vito; Unkles, Shiela E.; Brakhage, Axel A.

    2015-01-01

    Synthetic biology is an ever-expanding field in science, also encompassing the research area of fungal natural product (NP) discovery and production. Until now, different aspects of synthetic biology have been covered in fungal NP studies from the manipulation of different regulatory elements and heterologous expression of biosynthetic pathways to the engineering of different multidomain biosynthetic enzymes such as polyketide synthases or non-ribosomal peptide synthetases. The following review will cover some of the exemplary studies of synthetic biology in filamentous fungi showing the capacity of these eukaryotes to be used as model organisms in the field. From the vast array of different NPs produced to the ease for genetic manipulation, filamentous fungi have proven to be an invaluable source for the further development of synthetic biology tools. PMID:26284053

  10. Natural product synthesis at the interface of chemistry and biology.

    Science.gov (United States)

    Hong, Jiyong

    2014-08-11

    Nature has evolved to produce unique and diverse natural products that possess high target affinity and specificity. Natural products have been the richest sources for novel modulators of biomolecular function. Since the chemical synthesis of urea by Wöhler, organic chemists have been intrigued by natural products, leading to the evolution of the field of natural product synthesis over the past two centuries. Natural product synthesis has enabled natural products to play an essential role in drug discovery and chemical biology. With the introduction of novel, innovative concepts and strategies for synthetic efficiency, natural product synthesis in the 21st century is well poised to address the challenges and complexities faced by natural product chemistry and will remain essential to progress in biomedical sciences. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Editorial for Special Issue on Herbal Medicines and Natural Products.

    Science.gov (United States)

    Zhou, Zhi-Wei; Zhou, Shu-Feng

    2015-11-16

    Herbal medicines and natural products have been the most productive source of drug development and there is a large line of evidence on the applications of herbal medicines and natural products for the management of body function and the treatment of aliments. The multiple bioactive components in herbal medicines and natural products can explain the multiple targets effect in their medical applications. The increasing usage of state-of-art computational, molecular biological, and analytical chemistry techniques will promote the exploration of the pharmacological effect of previously inaccessible sources of herbal medicines and natural products. Notably, with the increasing reports on the safety issues regarding the medical use of herbal medicines and natural products, the awareness of pharmacovigilance in herbal medicines and natural products needs to be strengthened. To prevent the adverse drug reactions related to herbal medicines and natural products, physicians need to be aware of potential risks and alert patients in the use of herbal medicines and natural products.

  12. Synthetic studies on heterocyclic natural products.

    Science.gov (United States)

    Ciufolini, Marco A

    2005-08-01

    This article reviews past and ongoing research in the author's laboratory directed toward the synthesis of natural products displaying an azaspirocyclic framework, or incorporating a medium-ring nitrogen heterocycle. New synthetic technologies were devised in order to address the synthetic problems posed by the target molecules. Thus, efforts in the area of azaspirocyclic substances have relied on an oxidative amidation of phenols promoted by iodobenzene diacetate, whereas access to medium-ring nitrogen heterocycles has been secured by means of a ring expansion sequence that relies on the fragmentation of an aziridine triggered by a homo-Brook transposition. Details of the development of these technologies are presented, together with applications to the total synthesis of FR-901483, TAN-1251C, cylindricines, and mitomycinoids.

  13. Chocolate: A Marvelous Natural Product of Chemistry

    Science.gov (United States)

    Tannenbaum, Ginger

    2004-08-01

    Chocolate is a natural product as ubiquitous as television. Of course, it is eaten, but it is also found in air fresheners, marking pens, flavoring in a multitude of products including soda pop, and as an aroma in "chocolate-dyed" T-shirts. However, most of us are completely unaware of the complex chemical reactions that take place to produce chocolate and the necessary technology that has evolved to produce chocolate and all its byproducts. Processing results in a mixture of many components, an interesting contrast to most of the simple, one-step reactions introduced at the high school level. This article is a survey of chocolate from tree to table. After a brief introduction to the history of chocolate and how and where it is grown, the manufacturing process is examined, and the chemistry is explored. A bit of the jargon used in the industry is mentioned. Cocoa butter is a significant ingredient in chocolate, and an investigation of it introduces triglycerides, fatty acids, polymorphic behavior, and molecular packing of the fats in chocolate and how they affect the tempering process. There is a brief discussion of chocolate's non-Newtonian behavior and the resulting challenges presented in the manufacturing process. See Featured Molecules Featured on the Cover

  14. Cordyceps fungi: natural products, pharmacological functions and developmental products.

    Science.gov (United States)

    Zhou, Xuanwei; Gong, Zhenghua; Su, Ying; Lin, Juan; Tang, Kexuan

    2009-03-01

    Parasitic Cordyceps fungi, such as Cordyceps sinensis, is a parasitic complex of fungus and caterpillar, which has been used for medicinal purposes for centuries particularly in China, Japan and other Asian countries. This article gives a general idea of the latest developments in C. sinensis research, with regard to the active chemical components, the pharmacological effects and the research and development of products in recent years. The common names for preparations include DongChongXiaCao in Chinese, winter worm summer grass in English. It has many bioactive components, such as 3'-deoxyadenosine, cordycepic acid and Cordyceps polysaccharides. It is commonly used to replenish the kidney and soothe the lung, and for the treatment of fatigue. It also can be used to treat conditions such as night sweating, hyposexuality, hyperglycaemia, hyperlipidaemia, asthenia after severe illness, respiratory disease, renal dysfunction, renal failure, arrhythmias and other heart disease and liver disease. Because of its rarity and outstanding curative effects, several mycelia strains have been isolated from natural Cordyceps and manufactured by fermentation technology, and are commonly sold as health food products. In addition, some substitutes such as C. militaris and adulterants also have been used; therefore, quality control of C. sinensis and its products is very important to ensure their safety and efficacy. Recent research advances in the study of Cordyceps, including Cordyceps mushrooms, chemical components, pharmacological functions and developmental products, has been reviewed and discussed. Developing trends in the field have also been appraised.

  15. Synthetic studies toward complex Schisandraceae and zoanthamine natural products

    OpenAIRE

    Fischer, Derek A.

    2008-01-01

    Natural products, or secondary metabolites of plants and animals, have proven invaluable to humanity. We have used them for myriad reasons throughout history, including non- essential purposes such as dyes for textiles and paints. Less trivial uses, such as those related to food and health better demonstrate the importance of natural products. We have made use of toxic natural products to bolster hunting efficiency (e.g. poison tipped darts), and insect pheromones and natural products with an...

  16. Genomics-Driven Natural Product Discovery in Actinomycetes.

    Science.gov (United States)

    Niu, Guoqing

    2018-03-01

    The prevalence of antimicrobial-resistant pathogens has highlighted the urgent need for new drugs. Actinomycetes have been the most prominent sources of natural products for drug discovery and development. Advances in genomics have inspired several emerging strategies to reinvigorate the field of natural product discovery, especially in actinomycete-derived natural products. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Marine natural products as novel antioxidant prototypes.

    Science.gov (United States)

    Takamatsu, Satoshi; Hodges, Tyler W; Rajbhandari, Ira; Gerwick, William H; Hamann, Mark T; Nagle, Dale G

    2003-05-01

    Pure natural products isolated from marine sponges, algae, and cyanobacteria were examined for antioxidant activity using a 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) solution-based chemical assay and a 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) cellular-based assay. The DCFH system detects only antioxidants that penetrate cellular membranes. Potent antioxidants were identified and the results from each system compared. The algal metabolites cymopol (1), avrainvilleol (3), and fragilamide (4), and the invertebrate constituent puupehenone (5) showed strong antioxidant activity in both systems. Several compounds were active in the DPPH assay but significantly less active in the DCFH system. The green algal metabolite 7-hydroxycymopol (2) was isolated from Cymopolia barbataand its structure determined. Compound 2 was significantly less active in the DCFH system than cymopol (1). The sponge metabolites (1S)-(+)-curcuphenol (6), aaptamine (7), isoaaptamine (8), and curcudiol (9) and the cyanobacterial pigment scytonemin (10) showed strong antioxidant activity in the DPPH assay, but were relatively inactive in the DCFH system. Thus, cellular uptake dramatically affects the potential significance of antioxidants discovered using only the DPPH assay. The apparent "proantioxidants" hormothamnione A diacetate (11) and Laurencia monomer diacetate (12) require metabolic activation for antioxidant activity. Significant advantages are achieved using both a solution- and cellular-based assay to discover new antioxidants.

  18. Antifouling Activity of Marine Natural Products

    KAUST Repository

    Qian, Pei-Yuan

    2012-01-01

    With the global ban of application of organotin-based marine coatings by International Maritime Organization in 2008, there is a practical and urgent need of identifying environmentally friendly low-toxic and nontoxic antifouling compounds for marine industries. Marine natural products have been considered as one of the most promising sources of antifouling compounds in recent years. In antifouling compound screening processes, bioassay systems often play most critical/vital roles in screening efforts. To meet various needs, a variety of bioassay systems have been developed and/or adopted in both research and commercial laboratories. In this chapter, we provide a brief outline of common bioassay procedures for both antimicrofouling and antimacrofouling assays, which can serve as a general guideline for setting up bioassay systems in laboratories engaged in antifouling compound screening. Some bioassay procedures currently practiced in various laboratories are not included in this book chapter for various reasons. Individual laboratories should modify bioassay protocols based on their research interests or needs. Nevertheless, we highly recommend the research laboratories to adapt high-throughput assays as much as possible for preliminary screening assays, followed by more complex bioassay processes using multiple target species. We argue strongly for studies in mode-of-action of antifouling compounds against settling propagules, which shall lead to discovery of molecular biomarkers (genes, proteins, receptors, or receptor system) and will allow us to design more targeted bioassay systems.

  19. A Historical Overview of Natural Products in Drug Discovery

    Directory of Open Access Journals (Sweden)

    Daniel A. Dias

    2012-04-01

    Full Text Available Historically, natural products have been used since ancient times and in folklore for the treatment of many diseases and illnesses. Classical natural product chemistry methodologies enabled a vast array of bioactive secondary metabolites from terrestrial and marine sources to be discovered. Many of these natural products have gone on to become current drug candidates. This brief review aims to highlight historically significant bioactive marine and terrestrial natural products, their use in folklore and dereplication techniques to rapidly facilitate their discovery. Furthermore a discussion of how natural product chemistry has resulted in the identification of many drug candidates; the application of advanced hyphenated spectroscopic techniques to aid in their discovery, the future of natural product chemistry and finally adopting metabolomic profiling and dereplication approaches for the comprehensive study of natural product extracts will be discussed.

  20. Construction of a 3D-shaped, natural product like fragment library by fragmentation and diversification of natural products.

    Science.gov (United States)

    Prescher, Horst; Koch, Guido; Schuhmann, Tim; Ertl, Peter; Bussenault, Alex; Glick, Meir; Dix, Ina; Petersen, Frank; Lizos, Dimitrios E

    2017-02-01

    A fragment library consisting of 3D-shaped, natural product-like fragments was assembled. Library construction was mainly performed by natural product degradation and natural product diversification reactions and was complemented by the identification of 3D-shaped, natural product like fragments available from commercial sources. In addition, during the course of these studies, novel rearrangements were discovered for Massarigenin C and Cytochalasin E. The obtained fragment library has an excellent 3D-shape and natural product likeness, covering a novel, unexplored and underrepresented chemical space in fragment based drug discovery (FBDD). Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Pharmaceuticals from natural products: current trends

    Directory of Open Access Journals (Sweden)

    PETER R. SEIDL

    2002-03-01

    Full Text Available The use of products extracted from plants for medicinal purposes can be traced to the beginnings of civilization and up until the end of the nineteenth century natural products were the principal source of medicines. Since then their relative importance has oscillated according to the strategies of large pharmaceutical companies. Now that these strategies are changing, there are new opportunities for countries like Brazil, in which a large proportion of the world's biodiversity is located. There are, however, new circumstances that must be taken into consideration: material must be collected by groups which are formally authorized to do so and under the conditions of the Convention of Biological Diversity, the discovery process is being successively outsourced to smaller specialized firms and there is a growing integration with producers of cosmetics and phytomedicines.O uso de produtos extraídos de plantas para fins medicinais pode ser tracejado aos inícios da civilização e até o fim do século XIX, os produtos naturais constituíram a principal fonte de medicamentos. Desde então, a importância relativa dos produtos naturais tem oscilado de acordo com as estratégias de grandes companhias farmacêuticas. Agora que estas estratégias vêm mudando, há novas oportunidades para países como o Brasil, no qual está localizada uma vasta proporção da biodiversidade mundial. Há, entretanto, novas circunstâncias que devem ser levadas em consideração: o material deve ser coletado por grupos que estão formalmente autorizados para tal, com a coleta sendo feita sob as condições da Convenção de Diversidade Biológica, o processo de descoberta está sendo sucessivamente terceirizado no sentido de firmas especializadas menores e há uma integração crescente como produtores de cosméticos e fitomedicamentos.

  2. Natural Product Biosynthetic Diversity and Comparative Genomics of the Cyanobacteria.

    Science.gov (United States)

    Dittmann, Elke; Gugger, Muriel; Sivonen, Kaarina; Fewer, David P

    2015-10-01

    Cyanobacteria are an ancient lineage of slow-growing photosynthetic bacteria and a prolific source of natural products with intricate chemical structures and potent biological activities. The bulk of these natural products are known from just a handful of genera. Recent efforts have elucidated the mechanisms underpinning the biosynthesis of a diverse array of natural products from cyanobacteria. Many of the biosynthetic mechanisms are unique to cyanobacteria or rarely described from other organisms. Advances in genome sequence technology have precipitated a deluge of genome sequences for cyanobacteria. This makes it possible to link known natural products to biosynthetic gene clusters but also accelerates the discovery of new natural products through genome mining. These studies demonstrate that cyanobacteria encode a huge variety of cryptic gene clusters for the production of natural products, and the known chemical diversity is likely to be just a fraction of the true biosynthetic capabilities of this fascinating and ancient group of organisms. Copyright © 2015. Published by Elsevier Ltd.

  3. Industrial natural product chemistry for drug discovery and development.

    Science.gov (United States)

    Bauer, Armin; Brönstrup, Mark

    2014-01-01

    Covering: up to March 2013. In addition to their prominent role in basic biological and chemical research, natural products are a rich source of commercial products for the pharmaceutical and other industries. Industrial natural product chemistry is of fundamental importance for successful product development, as the vast majority (ca. 80%) of commercial drugs derived from natural products require synthetic efforts, either to enable economical access to bulk material, and/or to optimize drug properties through structural modifications. This review aims to illustrate issues on the pathway from lead to product, and how they have been successfully addressed by modern natural product chemistry. It is focused on natural products of current relevance that are, or are intended to be, used as pharmaceuticals.

  4. Applications of Ultra High Performance Liquid Chromatography for Natural Products

    International Nuclear Information System (INIS)

    Rosniza Razali; Hazlina Ahmad Hassali; Azfar Hanif Abd Aziz; Daryl Jesus Arapoc

    2015-01-01

    Ultra high performance liquid chromatography (UHPLC) is a very powerful and versatile chromatographic technique for the separation of natural products in complex matrices, such as crude extracts for quantification or general profiling. The method is widespread and has been adapted to the analysis of a broad range of natural products generally without the need for complex sample preparation. The present paper overviews regarding the UHPLC and its applications for natural products. (author)

  5. Biosynthesis of therapeutic natural products using synthetic biology.

    Science.gov (United States)

    Awan, Ali R; Shaw, William M; Ellis, Tom

    2016-10-01

    Natural products are a group of bioactive structurally diverse chemicals produced by microorganisms and plants. These molecules and their derivatives have contributed to over a third of the therapeutic drugs produced in the last century. However, over the last few decades traditional drug discovery pipelines from natural products have become far less productive and far more expensive. One recent development with promise to combat this trend is the application of synthetic biology to therapeutic natural product biosynthesis. Synthetic biology is a young discipline with roots in systems biology, genetic engineering, and metabolic engineering. In this review, we discuss the use of synthetic biology to engineer improved yields of existing therapeutic natural products. We further describe the use of synthetic biology to combine and express natural product biosynthetic genes in unprecedented ways, and how this holds promise for opening up completely new avenues for drug discovery and production. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. NPASS: natural product activity and species source database for natural product research, discovery and tool development.

    Science.gov (United States)

    Zeng, Xian; Zhang, Peng; He, Weidong; Qin, Chu; Chen, Shangying; Tao, Lin; Wang, Yali; Tan, Ying; Gao, Dan; Wang, Bohua; Chen, Zhe; Chen, Weiping; Jiang, Yu Yang; Chen, Yu Zong

    2018-01-04

    There has been renewed interests in the exploration of natural products (NPs) for drug discovery, and continuous investigations of the therapeutic claims and mechanisms of traditional and herbal medicines. In-silico methods have been employed for facilitating these studies. These studies and the optimization of in-silico algorithms for NP applications can be facilitated by the quantitative activity and species source data of the NPs. A number of databases collectively provide the structural and other information of ∼470 000 NPs, including qualitative activity information for many NPs, but only ∼4000 NPs are with the experimental activity values. There is a need for the activity and species source data of more NPs. We therefore developed a new database, NPASS (Natural Product Activity and Species Source) to complement other databases by providing the experimental activity values and species sources of 35 032 NPs from 25 041 species targeting 5863 targets (2946 proteins, 1352 microbial species and 1227 cell-lines). NPASS contains 446 552 quantitative activity records (e.g. IC50, Ki, EC50, GI50 or MIC mainly in units of nM) of 222 092 NP-target pairs and 288 002 NP-species pairs. NPASS, http://bidd2.nus.edu.sg/NPASS/, is freely accessible with its contents searchable by keywords, physicochemical property range, structural similarity, species and target search facilities. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  7. Collective synthesis of natural products by means of organocascade catalysis

    Science.gov (United States)

    Jones, Spencer B.; Simmons, Bryon; Mastracchio, Anthony; MacMillan, David W. C.

    2012-01-01

    Organic chemists are now able to synthesize small quantities of almost any known natural product, given sufficient time, resources and effort. However, translation of the academic successes in total synthesis to the large-scale construction of complex natural products and the development of large collections of biologically relevant molecules present significant challenges to synthetic chemists. Here we show that the application of two nature-inspired techniques, namely organocascade catalysis and collective natural product synthesis, can facilitate the preparation of useful quantities of a range of structurally diverse natural products from a common molecular scaffold. The power of this concept has been demonstrated through the expedient, asymmetric total syntheses of six well-known alkaloid natural products: strychnine, aspidospermidine, vincadifformine, akuammicine, kopsanone and kopsinine. PMID:21753848

  8. Synthesis of natural products of therapeutic significance

    Indian Academy of Sciences (India)

    system

    2015-11-07

    Nov 7, 2015 ... and some of them are validated for their activity against various diseases, thus allowing entry in to the drug discovery progression at an advanced stage. Unusual, efficacious bioactivity profile. (Interceptors of key cellular pathways and specific inhibitors/promoters of key enzymes). Nature: The master ...

  9. Natural Products and Dietary Prevention of Cancer

    Science.gov (United States)

    The concept of cancer prevention was first introduced in studies using the natural form of vitamin A in the prevention of epithelial cancers. Ever since, research on cancer prevention has grown and become a rather specialized field study. Cancer is a multistage process, and takes several years for...

  10. Learning Organic Chemistry Through Natural Products

    Indian Academy of Sciences (India)

    In order to keep the focus on chemistry, we defer giving its trivial name and plant source till the end of the discussion. An addi- ... The functional groups are to be determined now. The nature of the oxygen functionality is shown by a ... unaffected . SIRIIS I ARTICLI group since it undergoes acetylation. B moves higher with the.

  11. Five decades in the study of natural products

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences; Volume 114; Issue 3 ... Natural products; tylophora alkaloids; carpaine; tiliacorine; ishwarone; azadirachtin A. Abstract. This paper describes the five-decade long fascinating journey taken by Prof. T R Govindachari towards the study of the natural products of India. A variety of ...

  12. Synthesis of natural-product-based compound libraries

    NARCIS (Netherlands)

    Wessjohann, L.A.

    2000-01-01

    Natural products cover a diversity space not yet available from synthetic libraries, with an unrivalled success rate as drug leads. The combinatorial synthesis of non-oligomeric natural-product-based libraries, however, is still limited to few examples because access to easily modified units

  13. T R Seshadri's Contributions to the Chemistry of Natural Products

    Indian Academy of Sciences (India)

    Sri Sathya Sai Institute of. Higher Learning. Generations of students would vouch for the fact that he has the uncanny ability to present the chemistry of natural products logically and with feeling. Keywords. Flavonoids, lichen metabolite, methylation, Elbs-Seshadri oxi- dation, structure elucidation, natural products synthesis.

  14. Nigerian Journal of Natural Products and Medicine: Editorial Policies

    African Journals Online (AJOL)

    Focus and Scope. Nigerian Journal of Natural Products and Medicine is published by the Nigerian Society of Pharmacognosy, a non profit organisation established in 1982 dedicated to the promotion of Pharmacognosy, Natural Products and Traditional Medicine. It has a current circulation of about 500 to scientists in ...

  15. Phytotherapy natural products: Promotional mix features

    Directory of Open Access Journals (Sweden)

    Dugalić Sretenka L.

    2002-01-01

    Full Text Available Development of products takes into account organized and controlled planning of changes of inner self and toward the environment. That means the creation of an active force, which should bring changes in other words, it should change the person who participates in the process of creation or in the consumption of the product. It tried to induce customers to purchase through methodologies and promotion strategies which stand to our sen'ice by the change in the way of thinking of the medical and pharmaceutical profession and users of helpful means. The input of marketing within the control of the outcomes, leaders techniques and promotion strategies by the production of helpful healthy means has a very big meaning.

  16. Harnessing natural product assembly lines: structure, promiscuity, and engineering

    Science.gov (United States)

    Ladner, Christopher C; Williams, Gavin J

    2015-01-01

    Many therapeutically relevant natural products are biosynthesized by the action of giant mega-enzyme assembly lines. By leveraging the specificity, promiscuity, and modularity of assembly lines, a variety of strategies have been developed that enable the biosynthesis of modified natural products. This review briefly summarizes recent structural advances related to natural product assembly lines, discusses chemical approaches to probing assembly line structures in the absence of traditional biophysical data, and surveys efforts that harness the inherent or engineered promiscuity of assembly lines for the synthesis of non-natural polyketides and nonribosomal peptide analogues. PMID:26527577

  17. Harnessing natural product assembly lines: structure, promiscuity, and engineering.

    Science.gov (United States)

    Ladner, Christopher C; Williams, Gavin J

    2016-03-01

    Many therapeutically relevant natural products are biosynthesized by the action of giant mega-enzyme assembly lines. By leveraging the specificity, promiscuity, and modularity of assembly lines, a variety of strategies has been developed that enables the biosynthesis of modified natural products. This review briefly summarizes recent structural advances related to natural product assembly lines, discusses chemical approaches to probing assembly line structures in the absence of traditional biophysical data, and surveys efforts that harness the inherent or engineered promiscuity of assembly lines for the synthesis of non-natural polyketides and non-ribosomal peptide analogues.

  18. Antimycobacterial susceptibility testing methods for natural products research

    Directory of Open Access Journals (Sweden)

    Juan Gabriel Bueno Sánchez

    2010-06-01

    Full Text Available The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need of continuous developments on new and efficient methods to determine the susceptibility of isolates of M. tuberculosis in the search for novel antimicrobial agents. Natural products constitute an important source of new drugs, but design and implementation of antimycobacterial susceptibility testing methods are necessary for evaluate the different extracts and compounds. A number of biological assay methodologies are in current use, ranging from the classical disk diffusion and broth dilution assay format, to radiorespirometric (BACTEC, dye-based, and fluorescent/luminescence reporter assays. This review presents an analysis on the in vitro susceptibility testing methods developed for determinate antitubercular activity in natural products and related compounds (semi-synthetic natural products and natural products-derived compounds and the criteria to select the adequate method for determination of biological activity of new natural products.

  19. Organosilicon Reagents in Natural Product Synthesis

    Indian Academy of Sciences (India)

    Product Synthesis. Hari Prasad S teaches post graduate students medicinal organic chemistry and organic spectroscopy at the. Chemistry Department, ..... Structure 2. P.'-NH2. OH. KF/HCI/MeOH. JJ~. B~ H~ .. Tf = tli fluoromethane sui tonate. Nap = naphthyl. (R)-Isoprotcrenol. The silyl enol ethers undergo reaction with ...

  20. Natural products: the new engine for African trade growth

    OpenAIRE

    Bennett, Ben

    2006-01-01

    This report was to further develop the trade component of the Natural Resources Enterprise Programme (NATPRO). The field work was undertaken in Malawi, Zimbabwe, Namibia, the Republic of South Africa and the United Kingdom between 9th January and 25th February 2006. The work concentrated on ten countries in Southern Africa with potential to export significant quantities of natural products. These products are defined by the project as follows: being plant derived, occurring naturally, wild ha...

  1. NATURAL ANTIOXIDANTS IN PRODUCTION “BRUSHWOOD”

    Directory of Open Access Journals (Sweden)

    G. N. Dubtsova

    2015-01-01

    Full Text Available Flour wares use popularity, there is 48% of general volume of pastry wares on their stake. A marketing study on results that actuality of development of new type of flour good is set with the lowered maintenance of fat and enriched by vitamins is undertaken. The use of natural antioxidant of "Guarding is reasonable toco 70" and introduction of 0,2% is recommended for stabilizing of sunflower-seed oil at fry of frying. Joint introduction of powders is studied from the garden-stuffs of brier and unabi on quality of the brushwood fried in sunflower-seed oil with the use of "Guarding toco 70". From wares extracted lipids and estimated on indexes: peroxides, acid and anizid numbers. It is set that an antioxidant and vegetable powders assist the decline of indexes of safety peroxides and asid in two times, and anizid in 1,3 time. Use of antioxidant of "Guarding toco 70", being in 70% from the natural concentrate of mixture of tocopherolss and 30% of soyaoil.

  2. Spatial and temporal control of fungal natural product synthesis.

    Science.gov (United States)

    Lim, Fang Yun; Keller, Nancy P

    2014-10-01

    Despite their oftentimes-elusive ecological role, fungal natural products have, for better or worse, impacted our daily lives tremendously owing to their diverse and potent bioactive properties. This Janus-faced nature of fungal natural products inevitably ushered in a field of research dedicated towards understanding the ecology, organisms, genes, enzymes, and biosynthetic pathways that give rise to this arsenal of diverse and complex chemistry. Ongoing research in fungal secondary metabolism has not only increased our appreciation for fungal natural products as an asset but also sheds light on the pivotal role that these once-regarded "metabolic wastes" play in fungal biology, defense, and stress response in addition to their potential contributions towards human mycoses. Full orchestration of secondary metabolism requires not only the seamless coordination between temporal and spatial control of SM-associated machineries (e.g. enzymes, cofactors, intermediates, and end-products) but also integration of these machineries into primary metabolic processes and established cellular mechanisms. An intriguing, but little known aspect of microbial natural product synthesis lies in the spatial organization of both pathway intermediates and enzymes responsible for the production of these compounds. In this highlight, we summarize some major breakthroughs in understanding the genes and regulation of fungal natural product synthesis and introduce the current state of knowledge on the spatial and temporal control of fungal natural product synthesis.

  3. Linking neuroethology to the chemical biology of natural products

    DEFF Research Database (Denmark)

    Olivera, Baldomero M.; Raghuraman, Shrinivasan; Schmidt, Eric W.

    2017-01-01

    From a biological perspective, a natural product can be defined as a compound evolved by an organism for chemical interactions with another organism including prey, predator, competitor, pathogen, symbiont or host. Natural products hold tremendous potential as drug leads and have been extensively...... studied by chemists and biochemists in the pharmaceutical industry. However, the biological purpose for which a natural product evolved is rarely addressed. By focusing on a well-studied group of natural products—venom components from predatory marine cone snails—this review provides a rationale for why...... a better understanding of the evolution, biology and biochemistry of natural products will facilitate both neuroscience and the potential for drug leads. The larger goal is to establish a new sub-discipline in the broader field of neuroethology that we refer to as “Chemical Neuroethology”, linking...

  4. Biodiesel production through transesterification over natural calciums

    Energy Technology Data Exchange (ETDEWEB)

    Ngamcharussrivichai, Chawalit [Fuels Research Center, Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330 (Thailand); Center of Excellence for Petroleum, Petrochemicals and Advanced Materials, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330 (Thailand); Nunthasanti, Pramwit; Tanachai, Sithikorn; Bunyakiat, Kunchana [Fuels Research Center, Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330 (Thailand)

    2010-11-15

    Transesterification of palm kernel oil (PKO) with methanol over various natural calciums, including limestone calcite, cuttlebone, dolomite, hydroxyapatite, and dicalcium phosphate, has been investigated at 60 C and 1 atm. The study showed that dolomite, mainly consisting of CaCO{sub 3} and MgCO{sub 3}, is the most active catalyst. The calcination temperature largely affected the physicochemical properties, as evidenced by N{sub 2} adsorption-desorption measurement, TGA, SEM and XRD, and the transesterification performance of the resultant catalysts. It was found that the calcination of dolomite at 800 C resulted in a highly active mixed oxide. CaO was suggested to be the catalytically active site responsible for the methyl ester formation. Under the suitable reaction conditions, the amount of dolomite calcined at 800 C = 6 wt.% based on the weight of oil, the methanol/oil molar ratio = 30, and the reaction time = 3 h, the methyl ester content of 98.0% can be achieved. The calcined dolomite can be reused many times. The analyses of some important fuel properties indicated that the biodiesel produced had the properties that meet the standard of biodiesel and diesel fuel issued by the Department of Energy Business, Ministry of Energy, Thailand. (author)

  5. Natural products-friends or foes?

    Science.gov (United States)

    Margină, Denisa; Ilie, Mihaela; Grădinaru, Daniela; Androutsopoulos, Vasilis P; Kouretas, Demetrios; Tsatsakis, Aristidis M

    2015-08-05

    A trend in the general population has been observed in recent years regarding the orientation toward preventive measures in health; in this context the increased interest from the users and researchers concerning the active effect of food supplements on the health state and on longevity, is noticeable. All over the world, the consumption of natural foods and of vegetal supplements has increased spectacularly over the last 5-10 years. The decreased prevalence of cardio-vascular diseases associated with Mediterranean diet, as well as the French paradox convinced researchers to scientifically document the beneficial outcomes pointed out by traditional use of plants, and to try to develop supplements that would have the same positive effects as these noticed for diet components. The intense research dedicated to this topic revealed the fact that food supplements are linked to some problematic aspects, such as toxicological side effects when associated with classical synthetic drugs. The food supplement-drug interactions are submitted to complex issues regarding pharmacokinetic interactions leading to changes in absorption, distribution, metabolism and excretion processes with direct impact on effect and toxicological potential. The present review based on recent literature aims at discussing the food-drug interactions with direct impact on efficacy and toxicity of drugs. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  6. Solid-phase enrichment and analysis of electrophilic natural products

    Directory of Open Access Journals (Sweden)

    Frank Wesche

    2017-03-01

    Full Text Available In search for new natural products, which may lead to the development of new drugs for all kind of applications, novel methods are needed. Here we describe the identification of electrophilic natural products in crude extracts via their reactivity against azide as a nucleophile followed by their subsequent enrichment using a cleavable azide-reactive resin (CARR. Using this approach, natural products carrying epoxides and α,β-unsaturated enones as well as several unknown compounds were identified in crude extracts from entomopathogenic Photorhabdus bacteria.

  7. Genetic regulation and manipulation for natural product discovery.

    Science.gov (United States)

    Chen, Jianwei; Wu, Qihao; Hawas, Usama W; Wang, Hong

    2016-04-01

    Natural products are an important source of modern medical development, e.g., antibiotics, anticancers, immune modulators, etc. and will continue to be a powerful driving force for the discovery of novel potential drugs. In the heterologous hosts, natural products are biosynthesized using dedicated metabolic networks. By gene engineering, pathway reconstructing, and enzyme engineering, metabolic networks can be modified to synthesize novel compounds containing enhanced structural feature or produce a large quantity of known valuable bioactive compounds. The review introduces some important technical platforms and relevant examples of genetic regulation and manipulation to improve natural product titers or drive novel secondary metabolite discoveries.

  8. Biosynthesis of natural products containing β-amino acids.

    Science.gov (United States)

    Kudo, Fumitaka; Miyanaga, Akimasa; Eguchi, Tadashi

    2014-08-01

    Covering: up to January, 2014. We focus here on β-amino acids as components of complex natural products because the presence of β-amino acids produces structural diversity in natural products and provides characteristic architectures beyond those of ordinary α-L-amino acids, thus generating significant and unique biological functions in nature. In this review, we first survey the known bioactive β-amino acid-containing natural products including nonribosomal peptides, macrolactam polyketides, and nucleoside-β-amino acid hybrids. Next, the biosynthetic enzymes that form β-amino acids from α-amino acids and the de novo synthesis of β-amino acids are summarized. Then, the mechanisms of β-amino acid incorporation into natural products are reviewed. Because it is anticipated that the rational swapping of the β-amino acid moieties with various side chains and stereochemistries by biosynthetic engineering should lead to the creation of novel architectures and bioactive compounds, the accumulation of knowledge regarding β-amino acid-containing natural product biosynthetic machinery could have a significant impact in this field. In addition, genome mining of characteristic β-amino acid biosynthetic genes and unique β-amino acid incorporation machinery could lead to the discovery of new β-amino acid-containing natural products.

  9. Natural product proteomining, a quantitative proteomics platform, allows rapid discovery of biosynthetic gene clusters for different classes of natural products.

    Science.gov (United States)

    Gubbens, Jacob; Zhu, Hua; Girard, Geneviève; Song, Lijiang; Florea, Bogdan I; Aston, Philip; Ichinose, Koji; Filippov, Dmitri V; Choi, Young H; Overkleeft, Herman S; Challis, Gregory L; van Wezel, Gilles P

    2014-06-19

    Information on gene clusters for natural product biosynthesis is accumulating rapidly because of the current boom of available genome sequencing data. However, linking a natural product to a specific gene cluster remains challenging. Here, we present a widely applicable strategy for the identification of gene clusters for specific natural products, which we name natural product proteomining. The method is based on using fluctuating growth conditions that ensure differential biosynthesis of the bioactivity of interest. Subsequent combination of metabolomics and quantitative proteomics establishes correlations between abundance of natural products and concomitant changes in the protein pool, which allows identification of the relevant biosynthetic gene cluster. We used this approach to elucidate gene clusters for different natural products in Bacillus and Streptomyces, including a novel juglomycin-type antibiotic. Natural product proteomining does not require prior knowledge of the gene cluster or secondary metabolite and therefore represents a general strategy for identification of all types of gene clusters. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Use of Brown Algae to Demonstrate Natural Products Techniques.

    Science.gov (United States)

    Porter, Lee A.

    1985-01-01

    Background information is provided on the natural products found in marine organisms in general and the brown algae in particular. Also provided are the procedures needed to isolate D-mannitol (a primary metabolite) and cholesterol from brown algae. (JN)

  11. Marine organisms: an alternative source of potentially valuable natural products

    Directory of Open Access Journals (Sweden)

    Alphonse Kelecom

    1991-01-01

    Full Text Available This paper recalls the outcoming of marine natural products research and reviews a selection of marirne bioactive metabolites in current use together with promising trends in marine pharmacology.

  12. Emerging trends in the discovery of natural product antibacterials

    DEFF Research Database (Denmark)

    Bologa, Cristian G; Ursu, Oleg; Oprea, Tudor

    2013-01-01

    mechanisms. Special emphasis is given to the strengths, weaknesses, and opportunities in the natural product antibacterial drug discovery arena, and to emerging applications driven by advances in bioinformatics, chemical biology, and synthetic biology in concert with exploiting bacterial phenotypes....... These efforts have identified a critical mass of natural product antibacterial lead compounds and discovery technologies with high probability of successful implementation against emerging bacterial pathogens....

  13. Anti-Enterovirus 71 Agents of Natural Products

    Directory of Open Access Journals (Sweden)

    Liyan Wang

    2015-09-01

    Full Text Available This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005–2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded.

  14. Anti-Enterovirus 71 Agents of Natural Products.

    Science.gov (United States)

    Wang, Liyan; Wang, Junfeng; Wang, Lishu; Ma, Shurong; Liu, Yonghong

    2015-09-09

    This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005-2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded.

  15. The sustainable management of a productive natural capital

    DEFF Research Database (Denmark)

    Daubanes, Julien Xavier

    This paper examines an industry whose economic activity uses a natural capital on which its profit also relies. When such a productive natural capital has a limited capacity to recover from its exploitation, a free market tends to over-exploit it, calling for public intervention. The analysis...... is relevant, among other examples, to the case of naturebased tourism. I study the sustainable management of a productive natural capital: the conditions under which its exploitation generates maximum long-run social benefits; the various ways in which a regulator can implement such an exploitation; the rent...

  16. Nigerian Journal of Natural Products and Medicine - Vol 13 (2009)

    African Journals Online (AJOL)

    Nigerian Journal of Natural Products and Medicine. ... Quality control of Harpagophytum procumbens products using High Pressure Liquid Chromatography-Diode Array Detection (HPLC-DAD) · EMAIL FULL TEXT EMAIL FULL ... Patenting for economic development: how aware are Nigerian pharmaceutical researchers?

  17. The use of natural products for consumption in Denmark

    International Nuclear Information System (INIS)

    Strandberg, M.

    1996-05-01

    Through a questionnaire it was indicated that Danes use products from 'natural' ecosystems in an amount of approximately 2 kg per year. The most used products are berries and meat from game animals, whereas freshwater fish and wild mushrooms are less common in Danish kitchens. (au)

  18. Phenazines and natural products; Novel synthesis of saphenic acid

    DEFF Research Database (Denmark)

    Petersen, Lars; Jensen, Knud Jørgen; Nielsen, John

    1999-01-01

    The natural product saphenic acid (6-(1-hydroxyethyl)1-phenazinecarboxylic acid) was synthesized from readily accessible starting materials. The desired product was obtained in an overall yield of 22% for four steps with the key steps being formation of a diphenylamine, followed by cyclization...

  19. Nature-Inspired Design : Strategies for Sustainable Product Development

    NARCIS (Netherlands)

    De Pauw, I.C.

    2015-01-01

    Product designers can apply different strategies, methods, and tools for sustainable product development. Nature-Inspired Design Strategies (NIDS) offer designers a distinct class of strategies that use ‘nature’ as a guiding source of knowledge and inspiration for addressing sustainability.

  20. World statistics on natural gas reserves, production and utilization

    International Nuclear Information System (INIS)

    Raikaslehto, S.

    2001-01-01

    By reviewing the statistics of BP Amoco on natural gas reserves, production and usage, it is easy to see that Russia and USA, both being large natural gas producers, differ significantly from each other. The natural gas reserves of USA are 6th largest in the world, simultaneously the natural gas consumption and import are largest in the world. About one third of the known natural gas reserves of the world are in Russia. The known natural gas reserves of both USA and Canada have decreases, but they have potential gas reserves left. Known natural gas reserves of the USA have been calculated to be sufficient for 9 years consumption at present usage and those of Canada for 11 years. The reserves of Algeria correspond to the usage of 55 years, and the Russian reserves for are about 83 years. Annual production figures of both Russia and the USA are nearly the same. Russia is the largest exporter (125.5 billion m 3 ) of natural gas and the USA the largest importer (96 billion m 3 ). The natural gas reserves of the largest European producers, the Netherlands and Norway have been estimated to be sufficient for use of about 20 years, but those of Great Britain only for about 10 years. The annual production of Russia has varied in the 1990s between nearly 600 billion m 3 and present 550 billion m 3 , the minimum being in 1997 only about 532 billion m 3 . Ten largest natural gas consumers use 67% of the natural gas consumed annually in the world. USA consumes about 27% of the total natural gas produced in the world, the amount of Russia being 364 billion m 3 (16%). Other large natural gas consumers are Great Britain, Germany, Japan, Ukraine, Canada, Italy, Iran and Uzbekistan. The share of these countries of the total consumption varied in between 2-4%. Only Japan has no natural gas production of its own. The foreign trade between Japan and Indonesia is trade on LNG. On the other hand the natural gas consumption of the world's 10th largest producer Norway is nearly zero, so

  1. Natural product-based nanomedicine: recent advances and issues.

    Science.gov (United States)

    Watkins, Rebekah; Wu, Ling; Zhang, Chenming; Davis, Richey M; Xu, Bin

    2015-01-01

    Natural products have been used in medicine for many years. Many top-selling pharmaceuticals are natural compounds or their derivatives. These plant- or microorganism-derived compounds have shown potential as therapeutic agents against cancer, microbial infection, inflammation, and other disease conditions. However, their success in clinical trials has been less impressive, partly due to the compounds' low bioavailability. The incorporation of nanoparticles into a delivery system for natural products would be a major advance in the efforts to increase their therapeutic effects. Recently, advances have been made showing that nanoparticles can significantly increase the bioavailability of natural products both in vitro and in vivo. Nanotechnology has demonstrated its capability to manipulate particles in order to target specific areas of the body and control the release of drugs. Although there are many benefits to applying nanotechnology for better delivery of natural products, it is not without issues. Drug targeting remains a challenge and potential nanoparticle toxicity needs to be further investigated, especially if these systems are to be used to treat chronic human diseases. This review aims to summarize recent progress in several key areas relevant to natural products in nanoparticle delivery systems for biomedical applications.

  2. Natural product-based nanomedicine: recent advances and issues

    Science.gov (United States)

    Watkins, Rebekah; Wu, Ling; Zhang, Chenming; Davis, Richey M; Xu, Bin

    2015-01-01

    Natural products have been used in medicine for many years. Many top-selling pharmaceuticals are natural compounds or their derivatives. These plant- or microorganism-derived compounds have shown potential as therapeutic agents against cancer, microbial infection, inflammation, and other disease conditions. However, their success in clinical trials has been less impressive, partly due to the compounds’ low bioavailability. The incorporation of nanoparticles into a delivery system for natural products would be a major advance in the efforts to increase their therapeutic effects. Recently, advances have been made showing that nanoparticles can significantly increase the bioavailability of natural products both in vitro and in vivo. Nanotechnology has demonstrated its capability to manipulate particles in order to target specific areas of the body and control the release of drugs. Although there are many benefits to applying nanotechnology for better delivery of natural products, it is not without issues. Drug targeting remains a challenge and potential nanoparticle toxicity needs to be further investigated, especially if these systems are to be used to treat chronic human diseases. This review aims to summarize recent progress in several key areas relevant to natural products in nanoparticle delivery systems for biomedical applications. PMID:26451111

  3. Environmental policy and regulatory constraints to natural gas production.

    Energy Technology Data Exchange (ETDEWEB)

    Elcock, D.

    2004-12-17

    For the foreseeable future, most of the demand for natural gas in the United States will be met with domestic resources. Impediments, or constraints, to developing, producing, and delivering these resources can lead to price increases or supply disruptions. Previous analyses have identified lack of access to natural gas resources on federal lands as such an impediment. However, various other environmental constraints, including laws, regulations, and implementation procedures, can limit natural gas development and production on both federal and private lands. This report identifies and describes more than 30 environmental policy and regulatory impediments to domestic natural gas production. For each constraint, the source and type of impact are presented, and when the data exist, the amount of gas affected is also presented. This information can help decision makers develop and support policies that eliminate or reduce the impacts of such constraints, help set priorities for regulatory reviews, and target research and development efforts to help the nation meet its natural gas demands.

  4. Bioengineering natural product biosynthetic pathways for therapeutic applications.

    Science.gov (United States)

    Wu, Ming-Cheng; Law, Brian; Wilkinson, Barrie; Micklefield, Jason

    2012-12-01

    With the advent of next-generation DNA sequencing technologies, the number of microbial genome sequences has increased dramatically, revealing a vast array of new biosynthetic gene clusters. Genomics data provide a tremendous opportunity to discover new natural products, and also to guide the bioengineering of new and existing natural product scaffolds for therapeutic applications. Notably, it is apparent that the vast majority of biosynthetic gene clusters are either silent or produce very low quantities of the corresponding natural products. It is imperative therefore to devise methods for activating unproductive biosynthetic pathways to provide the quantities of natural products needed for further development. Moreover, on the basis of our expanding mechanistic and structural knowledge of biosynthetic assembly-line enzymes, new strategies for re-programming biosynthetic pathways have emerged, resulting in focused libraries of modified products with potentially improved biological properties. In this review we will focus on the latest bioengineering approaches that have been utilised to optimise yields and increase the structural diversity of natural product scaffolds for future clinical applications. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Natural Health Products and Community Pharmacy-Remove the Mysticism Not the Product.

    Science.gov (United States)

    Blackburn, David F; Gill, Munpreet; Krol, Ed; Taylor, Jeff

    2017-12-01

    The allure of natural products has captivated humans for centuries. Although they can be compatible with evidence-based care, attitudes surrounding natural products can seem almost mystical and may even be accompanied by contempt toward Western medicine. Considering the high volumes of natural products sold in community pharmacies, pharmacists can inject balanced information to minimize the mysticism and help patients make informed decisions. The aim of this article is to argue for standardized guidelines pertaining to the management of natural products in community pharmacy practice.

  6. A Growing Disconnection From Nature Is Evident in Cultural Products.

    Science.gov (United States)

    Kesebir, Selin; Kesebir, Pelin

    2017-03-01

    Human connection with nature is widely believed to be in decline even though empirical evidence is scarce on the magnitude and historical pattern of the change. Studying works of popular culture in English throughout the 20th century and later, we have documented a cultural shift away from nature that begins in the 1950s. Since then, references to nature have been decreasing steadily in fiction books, song lyrics, and film storylines, whereas references to the human-made environment have not. The observed temporal pattern is consistent with the explanatory role of increased virtual and indoors recreation options (e.g., television, video games) in the disconnect from nature, and it is inconsistent with a pure urbanization account. These findings are cause for concern, not only because they imply foregone physical and psychological benefits from engagement with nature, but also because cultural products are agents of socialization that can evoke curiosity, respect, and concern for the natural world.

  7. Natural product-based nanomedicine: recent advances and issues

    Directory of Open Access Journals (Sweden)

    Watkins R

    2015-09-01

    Full Text Available Rebekah Watkins,1,2,* Ling Wu,1,* Chenming Zhang,3–5 Richey M Davis,3,5,6 Bin Xu1,3 1Department of Biochemistry, 2Program in Nanoscience, 3Center for Drug Discovery, 4Department of Biological Systems Engineering, 5Institute for Critical Technology and Applied Science, 6Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA *These authors contributed equally to this work Abstract: Natural products have been used in medicine for many years. Many top-selling pharmaceuticals are natural compounds or their derivatives. These plant- or microorganism-derived compounds have shown potential as therapeutic agents against cancer, microbial infection, inflammation, and other disease conditions. However, their success in clinical trials has been less impressive, partly due to the compounds’ low bioavailability. The incorporation of nanoparticles into a delivery system for natural products would be a major advance in the efforts to increase their therapeutic effects. Recently, advances have been made showing that nanoparticles can significantly increase the bioavailability of natural products both in vitro and in vivo. Nanotechnology has demonstrated its capability to manipulate particles in order to target specific areas of the body and control the release of drugs. Although there are many benefits to applying nanotechnology for better delivery of natural products, it is not without issues. Drug targeting remains a challenge and potential nanoparticle toxicity needs to be further investigated, especially if these systems are to be used to treat chronic human diseases. This review aims to summarize recent progress in several key areas relevant to natural products in nanoparticle delivery systems for biomedical applications. Keywords: natural products, nanomedicine, drug delivery, bioavailability, targeting, controlled release

  8. Culture-independent discovery of natural products from soil metagenomes.

    Science.gov (United States)

    Katz, Micah; Hover, Bradley M; Brady, Sean F

    2016-03-01

    Bacterial natural products have proven to be invaluable starting points in the development of many currently used therapeutic agents. Unfortunately, traditional culture-based methods for natural product discovery have been deemphasized by pharmaceutical companies due in large part to high rediscovery rates. Culture-independent, or "metagenomic," methods, which rely on the heterologous expression of DNA extracted directly from environmental samples (eDNA), have the potential to provide access to metabolites encoded by a large fraction of the earth's microbial biosynthetic diversity. As soil is both ubiquitous and rich in bacterial diversity, it is an appealing starting point for culture-independent natural product discovery efforts. This review provides an overview of the history of soil metagenome-driven natural product discovery studies and elaborates on the recent development of new tools for sequence-based, high-throughput profiling of environmental samples used in discovering novel natural product biosynthetic gene clusters. We conclude with several examples of these new tools being employed to facilitate the recovery of novel secondary metabolite encoding gene clusters from soil metagenomes and the subsequent heterologous expression of these clusters to produce bioactive small molecules.

  9. The Traditional Medicine and Modern Medicine from Natural Products

    Directory of Open Access Journals (Sweden)

    Haidan Yuan

    2016-04-01

    Full Text Available Natural products and traditional medicines are of great importance. Such forms of medicine as traditional Chinese medicine, Ayurveda, Kampo, traditional Korean medicine, and Unani have been practiced in some areas of the world and have blossomed into orderly-regulated systems of medicine. This study aims to review the literature on the relationship among natural products, traditional medicines, and modern medicine, and to explore the possible concepts and methodologies from natural products and traditional medicines to further develop drug discovery. The unique characteristics of theory, application, current role or status, and modern research of eight kinds of traditional medicine systems are summarized in this study. Although only a tiny fraction of the existing plant species have been scientifically researched for bioactivities since 1805, when the first pharmacologically-active compound morphine was isolated from opium, natural products and traditional medicines have already made fruitful contributions for modern medicine. When used to develop new drugs, natural products and traditional medicines have their incomparable advantages, such as abundant clinical experiences, and their unique diversity of chemical structures and biological activities.

  10. Capturing Biological Activity in Natural Product Fragments by Chemical Synthesis.

    Science.gov (United States)

    Crane, Erika A; Gademann, Karl

    2016-03-14

    Natural products have had an immense influence on science and have directly led to the introduction of many drugs. Organic chemistry, and its unique ability to tailor natural products through synthesis, provides an extraordinary approach to unlock the full potential of natural products. In this Review, an approach based on natural product derived fragments is presented that can successfully address some of the current challenges in drug discovery. These fragments often display significantly reduced molecular weights, reduced structural complexity, a reduced number of synthetic steps, while retaining or even improving key biological parameters such as potency or selectivity. Examples from various stages of the drug development process up to the clinic are presented. In addition, this process can be leveraged by recent developments such as genome mining, antibody-drug conjugates, and computational approaches. All these concepts have the potential to identify the next generation of drug candidates inspired by natural products. © 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  11. The Traditional Medicine and Modern Medicine from Natural Products.

    Science.gov (United States)

    Yuan, Haidan; Ma, Qianqian; Ye, Li; Piao, Guangchun

    2016-04-29

    Natural products and traditional medicines are of great importance. Such forms of medicine as traditional Chinese medicine, Ayurveda, Kampo, traditional Korean medicine, and Unani have been practiced in some areas of the world and have blossomed into orderly-regulated systems of medicine. This study aims to review the literature on the relationship among natural products, traditional medicines, and modern medicine, and to explore the possible concepts and methodologies from natural products and traditional medicines to further develop drug discovery. The unique characteristics of theory, application, current role or status, and modern research of eight kinds of traditional medicine systems are summarized in this study. Although only a tiny fraction of the existing plant species have been scientifically researched for bioactivities since 1805, when the first pharmacologically-active compound morphine was isolated from opium, natural products and traditional medicines have already made fruitful contributions for modern medicine. When used to develop new drugs, natural products and traditional medicines have their incomparable advantages, such as abundant clinical experiences, and their unique diversity of chemical structures and biological activities.

  12. Exploring cyanobacterial genomes for natural product biosynthesis pathways.

    Science.gov (United States)

    Micallef, Melinda L; D'Agostino, Paul M; Al-Sinawi, Bakir; Neilan, Brett A; Moffitt, Michelle C

    2015-06-01

    Cyanobacteria produce a vast array of natural products, some of which are toxic to human health, while others possess potential pharmaceutical activities. Genome mining enables the identification and characterisation of natural product gene clusters; however, the current number of cyanobacterial genomes remains low compared to other phyla. There has been a recent effort to rectify this issue by increasing the number of sequenced cyanobacterial genomes. This has enabled the identification of biosynthetic gene clusters for structurally diverse metabolites, including non-ribosomal peptides, polyketides, ribosomal peptides, UV-absorbing compounds, alkaloids, terpenes and fatty acids. While some of the identified biosynthetic gene clusters correlate with known metabolites, genome mining also highlights the number and diversity of clusters for which the product is unknown (referred to as orphan gene clusters). A number of bioinformatic tools have recently been developed in order to predict the products of orphan gene clusters; however, in some cases the complexity of the cyanobacterial pathways makes the prediction problematic. This can be overcome by the use of mass spectrometry-guided natural product genome mining, or heterologous expression. Application of these techniques to cyanobacterial natural product gene clusters will be explored. Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.

  13. Radon gas in oil and natural gas production facilities

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, W.P. [Western Radiation Services, Perth, WA (Australia)

    1994-12-31

    Radon gas is a naturally occurring radionuclide that can be found in some oil and natural gas production facilities, either as a contaminant in a natural gas stream or derived from Radium dissolved in formation waters. The gas itself is not normally a health hazard, but it`s decay products, which can be concentrated by plate-out or deposition as a scale in process equipment, can be a health hazard for maintenance personnel. To evaluate possible health hazards, it is necessary to monitor for naturally occurring radioactive materials (NORM) in the gas stream and in the formation water. If Radon and/or Radium is found, a monitoring programme should be initiated to comply with National or State requirements. In some instances, it has been found necessary to dispose of silt and scale materials as low level radioactive waste. 8 refs.

  14. Dietary Natural Products for Prevention and Treatment of Liver Cancer

    Directory of Open Access Journals (Sweden)

    Yue Zhou

    2016-03-01

    Full Text Available Liver cancer is the most common malignancy of the digestive system with high death rate. Accumulating evidences suggests that many dietary natural products are potential sources for prevention and treatment of liver cancer, such as grapes, black currant, plum, pomegranate, cruciferous vegetables, French beans, tomatoes, asparagus, garlic, turmeric, ginger, soy, rice bran, and some edible macro-fungi. These dietary natural products and their active components could affect the development and progression of liver cancer in various ways, such as inhibiting tumor cell growth and metastasis, protecting against liver carcinogens, immunomodulating and enhancing effects of chemotherapeutic drugs. This review summarizes the potential prevention and treatment activities of dietary natural products and their major bioactive constituents on liver cancer, and discusses possible mechanisms of action.

  15. Pro-oxidant natural products as anticancer agents.

    Science.gov (United States)

    Martin-Cordero, Carmen; Leon-Gonzalez, Antonio Jose; Calderon-Montano, Jose Manuel; Burgos-Moron, Estefania; Lopez-Lazaro, Miguel

    2012-07-01

    Cancer cells produce high levels of reactive oxygen species (ROS) that lead to a state of increased basal oxidative stress. Since this state of oxidative stress makes cancer cells vulnerable to agents that further augment ROS levels, the use of pro-oxidant agents is emerging as an exciting strategy to selectively target tumor cells. Natural products have provided a significant contribution to the development of several drugs currently used in cancer chemotherapy. Although many natural products are known to affect the redox state of the cell, most studies on these compounds have focused on their antioxidant activity instead of on their pro-oxidant properties. This article provides an overview of natural products with pro-oxidant and anticancer activities, with special focus on plant secondary metabolites, and discusses their possible use as cancer chemotherapeutic agents.

  16. In situ natural product discovery via an artificial marine sponge.

    Science.gov (United States)

    La Clair, James J; Loveridge, Steven T; Tenney, Karen; O'Neil-Johnson, Mark; Chapman, Eli; Crews, Phillip

    2014-01-01

    There is continuing international interest in exploring and developing the therapeutic potential of marine-derived small molecules. Balancing the strategies for ocean based sampling of source organisms versus the potential to endanger fragile ecosystems poses a substantial challenge. In order to mitigate such environmental impacts, we have developed a deployable artificial sponge. This report provides details on its design followed by evidence that it faithfully recapitulates traditional natural product collection protocols. Retrieving this artificial sponge from a tropical ecosystem after deployment for 320 hours afforded three actin-targeting jasplakinolide depsipeptides that had been discovered two decades earlier using traditional sponge specimen collection and isolation procedures. The successful outcome achieved here could reinvigorate marine natural products research, by producing new environmentally innocuous sources of natural products and providing a means to probe the true biosynthetic origins of complex marine-derived scaffolds.

  17. Genomic basis for natural product biosynthetic diversity in the actinomycetes†

    Science.gov (United States)

    Nett, Markus; Ikeda, Haruo; Moore, Bradley S.

    2010-01-01

    The phylum Actinobacteria hosts diverse high G + C, Gram-positive bacteria that have evolved a complex chemical language of natural product chemistry to help navigate their fascinatingly varied lifestyles. To date, 71 Actinobacteria genomes have been completed and annotated, with the vast majority representing the Actinomycetales, which are the source of numerous antibiotics and other drugs from genera such as Streptomyces, Saccharopolyspora and Salinispora. These genomic analyses have illuminated the secondary metabolic proficiency of these microbes – underappreciated for years based on conventional isolation programs – and have helped set the foundation for a new natural product discovery paradigm based on genome mining. Trends in the secondary metabolomes of natural product-rich actinomycetes are highlighted in this review article, which contains 199 references. PMID:19844637

  18. Dereplication of Microbial Natural Products by LC-DAD-TOFMS

    DEFF Research Database (Denmark)

    Nielsen, Kristian Fog; Månsson, Maria; Rank, Christian

    2011-01-01

    Dereplication, the rapid identification of known compounds present in a mixture, is crucial to the fast discovery of novel natural products. Determining the elemental composition of compounds in mixtures and tentatively identifying natural products using MS/MS and UV/vis spectra is becoming easier...... with advances in analytical equipment and better compound databases. Here we demonstrate the use of LC-UV/vis-MS-based dereplication using data from UV/vis diode array detection and ESI+/ESI– time-of-flight MS for assignment of 719 microbial natural product and mycotoxin reference standards. ESI+ was the most...... versatile ionization method, detecting 93% of the compounds, although with 12% ionizing poorly. Using ESI+ alone, 56.1% of the compounds could be unambiguously assigned based on characteristic patterns of multiple adduct ions. Using ESI–, 36.4% of the compounds could have their molecular mass assigned...

  19. Anti-cancer natural products isolated from chinese medicinal herbs

    Directory of Open Access Journals (Sweden)

    Wu Guosheng

    2011-07-01

    Full Text Available Abstract In recent years, a number of natural products isolated from Chinese herbs have been found to inhibit proliferation, induce apoptosis, suppress angiogenesis, retard metastasis and enhance chemotherapy, exhibiting anti-cancer potential both in vitro and in vivo. This article summarizes recent advances in in vitro and in vivo research on the anti-cancer effects and related mechanisms of some promising natural products. These natural products are also reviewed for their therapeutic potentials, including flavonoids (gambogic acid, curcumin, wogonin and silibinin, alkaloids (berberine, terpenes (artemisinin, β-elemene, oridonin, triptolide, and ursolic acid, quinones (shikonin and emodin and saponins (ginsenoside Rg3, which are isolated from Chinese medicinal herbs. In particular, the discovery of the new use of artemisinin derivatives as excellent anti-cancer drugs is also reviewed.

  20. Knowledge based decision making: perspective on natural gas production

    Energy Technology Data Exchange (ETDEWEB)

    Ydstie, B. Erik; Stuland, Kjetil M.

    2009-07-01

    Conclusions (drawn by the author): Decarbonization of energy sources - From coal to renewable. Natural Gas Abundantly available - Norway is no. 3 exporter. Natural gas important as - Hydrogen source for chemicals; - Electricity; - End consumer usage (heating etc). Large potential for application of model based decision making; - Where and when to install platforms and drill wells - How to operate platforms and pipeline systems; - How to operate and optimize chemical production; - Optimization of electricity generation systems. (author)

  1. Exposures from consumption of agricultural and semi-natural products

    International Nuclear Information System (INIS)

    Strand, P.; Skuterud, L.; Balonov, M.; Travnikova, I.; Hove, K.; Howard, B.; Prister, B.S.; Ratnikov, A.

    1996-01-01

    The importance of food from different production systems to the internal dose from radiocesium, was investigated in selected study sites in Ukraine and Russia. Food products from semi-natural ecosystems are major contributors to the individual internal dose to rural population in areas affected by the Chernobyl accident. At the selected study sites it is estimated in 1995 that foods from private farms and forests contribute on average 35% to 60%, to the individual internal dose, variation relating to soil types and implemented countermeasures. The importance of food products from private farms and particularly forest products increases with time since Cs concentration in some of the natural food products have longer ecological half life than food products from agricultural systems. A significant relationship was observed between consumption of mushrooms and whole body content of radiocesium in rural people. The contribution to the collective dose of food products produced in the semi-natural ecosystems is less than the contribution to the individual internal dose for the local rural population

  2. LC-NMR: profiling and dereplication of natural product extracts

    International Nuclear Information System (INIS)

    Urban, Sylvia

    2006-01-01

    Natural products have served as a major source of drugs for centuries, with over half of the pharmaceuticals in use today derived from natural origins. Natural products continue to play a dominant role in the discovery of leads for the development of drugs for the treatment of human diseases. Much remains to be explored, particularly the marine and microbial environments, from which a host of novel bioactive chemical entities await discovery. The search for new drugs from natural origins (either terrestrial or marine) involves screening of extracts for the presence of novel compounds and an investigation of their biological activities. Suspected novel or bioactive compounds are usually isolated to elucidate the structure and for further biological and toxicological testing. The path that leads from the intact terrestrial or marine organism to the pure constituents is long, involving work that might last from weeks to years. Recognition of natural products at the earliest possible stage of separation is known as dereplication and is essential to avoid the time-consuming isolation of common constituents and nuisance compounds. In the search for new natural products, crude extracts are typically subjected to multi-step work-up and isolation procedures, which include various separation methods, in order to obtain pure compounds whose structure is then elucidated using off-line spectroscopic methods such as nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The characterisation of a natural product can be summarised by the information obtained from each of the individual spectroscopic techniques. With the application of one or more of these individual techniques a dereplication by partial characterisation is possible. As natural product extracts often contain a large number of closely related, and thus difficult to separate, compounds, this classical approach may become very tedious and time-consuming. The characterisation of natural products in complex

  3. The impact of natural products upon modern drug discovery.

    Science.gov (United States)

    Ganesan, A

    2008-06-01

    In the period 1970-2006, a total of 24 unique natural products were discovered that led to an approved drug. We analyze these successful leads in terms of drug-like properties, and show that they can be divided into two equal subsets. The first falls in the 'Lipinski universe' and complies with the Rule of Five. The second is a 'parallel universe' that violates the rules. Nevertheless, the latter compounds remain largely compliant in terms of logP and H-bond donors, highlighting the importance of these two metrics in predicting bioavailability. Natural products are often cited as an exception to Lipinski's rules. We believe this is because nature has learned to maintain low hydrophobicity and intermolecular H-bond donating potential when it needs to make biologically active compounds with high molecular weight and large numbers of rotatable bonds. In addition, natural products are more likely than purely synthetic compounds to resemble biosynthetic intermediates or endogenous metabolites, and hence take advantage of active transport mechanisms. Interestingly, the natural product leads in the Lipinski and parallel universe had an identical success rate (50%) in delivering an oral drug.

  4. NATURAL PRODUCTS: A CONTINUING SOURCE OF NOVEL DRUG LEADS

    Science.gov (United States)

    Cragg, Gordon M.; Newman, David J.

    2013-01-01

    1. Background Nature has been a source of medicinal products for millennia, with many useful drugs developed from plant sources. Following discovery of the penicillins, drug discovery from microbial sources occurred and diving techniques in the 1970s opened the seas. Combinatorial chemistry (late 1980s), shifted the focus of drug discovery efforts from Nature to the laboratory bench. 2. Scope of Review This review traces natural products drug discovery, outlining important drugs from natural sources that revolutionized treatment of serious diseases. It is clear Nature will continue to be a major source of new structural leads, and effective drug development depends on multidisciplinary collaborations. 3. Major Conclusions The explosion of genetic information led not only to novel screens, but the genetic techniques permitted the implementation of combinatorial biosynthetic technology and genome mining. The knowledge gained has allowed unknown molecules to be identified. These novel bioactive structures can be optimized by using combinatorial chemistry generating new drug candidates for many diseases. 4 General Significance: The advent of genetic techniques that permitted the isolation / expression of biosynthetic cassettes from microbes may well be the new frontier for natural products lead discovery. It is now apparent that biodiversity may be much greater in those organisms. The numbers of potential species involved in the microbial world are many orders of magnitude greater than those of plants and multi-celled animals. Coupling these numbers to the number of currently unexpressed biosynthetic clusters now identified (>10 per species) the potential of microbial diversity remains essentially untapped. PMID:23428572

  5. Natural product terpenoids in Eocene and Miocene conifer fossils.

    Science.gov (United States)

    Otto, Angelika; White, James D; Simoneit, Bernd R T

    2002-08-30

    Numerous saturated and aromatic hydrocarbons, but not polar compounds, originating from plants and microorganisms (biomarkers) have been reported in sediments, coals, and petroleum. Here we describe natural product terpenoids found in two fossil conifers, Taxodium balticum (Eocene) and Glyptostrobus oregonensis (Miocene). A similar terpenoid pattern is also observed in extant Taxodium distichum. The preservation of characteristic terpenoids (unaltered natural products) in the fossil conifers supports their systematic assignment to the Cypress family (Cupressaceae sensu lato). The results also show that fossil conifers can contain polar terpenoids, which are valuable markers for (paleo)chemosystematics and phylogeny.

  6. Structure, Chemical Synthesis, and Biosynthesis of Prodiginine Natural Products.

    Science.gov (United States)

    Hu, Dennis X; Withall, David M; Challis, Gregory L; Thomson, Regan J

    2016-07-27

    The prodiginine family of bacterial alkaloids is a diverse set of heterocyclic natural products that have likely been known to man since antiquity. In more recent times, these alkaloids have been discovered to span a wide range of chemical structures that possess a number of interesting biological activities. This review provides a comprehensive overview of research undertaken toward the isolation and structural elucidation of the prodiginine family of natural products. Additionally, research toward chemical synthesis of the prodiginine alkaloids over the last several decades is extensively reviewed. Finally, the current, evidence-based understanding of the various biosynthetic pathways employed by bacteria to produce prodiginine alkaloids is summarized.

  7. Synthesis and Biological Investigation of Antioxidant Pyrrolomorpholine Spiroketal Natural Products

    Science.gov (United States)

    Verano, Alyssa Leigh

    The pyrrolomorpholine spiroketal natural product family is comprised of epimeric furanose and pyranose isomers. These compounds were isolated from diverse plant species, all of which are used as traditional Chinese medicines for the treatment of a variety of diseases. Notably, the spiroketal natural products acortatarins A and B exhibit antioxidant activity in a diabetic renal cell model, significantly attenuating hyperglycemia-induced production of reactive oxygen species (ROS), a hallmark of diabetic nephropathy. The xylapyrrosides, additional members of the family, also inhibit t-butyl hydroperoxide-induced cytotoxicity in rat vascular smooth muscle cells. Accordingly, these natural products have therapeutic potential for the treatment of oxidative stress-related pathologies, and synthetic access would provide an exciting opportunity to investigate bioactivity and mechanism of action. Herein, we report the stereoselective synthesis of acortatarins A and B, furanose members of the pyrrolomorpholine spiroketal family. Our synthetic route was expanded to synthesize the pyranose congeners, thus completing entire D-enantiomeric family of natural products. Efficient access towards these scaffolds enabled systematic analogue synthesis, investigation of mechanism-of-action, and the discovery of novel antioxidants.

  8. European energy security: The future of Norwegian natural gas production

    International Nuclear Information System (INIS)

    Soederbergh, Bengt; Jakobsson, Kristofer; Aleklett, Kjell

    2009-01-01

    The European Union (EU) is expected to meet its future growing demand for natural gas by increased imports. In 2006, Norway had a 21% share of EU gas imports. The Norwegian government has communicated that Norwegian gas production will increase by 25-40% from today's level of about 99 billion cubic meters (bcm)/year. This article shows that only a 20-25% growth of Norwegian gas production is possible due to production from currently existing recoverable reserves and contingent resources. A high and a low production forecast for Norwegian gas production is presented. Norwegian gas production exported by pipeline peaks between 2015 and 2016, with minimum peak production in 2015 at 118 bcm/year and maximum peak production at 127 bcm/year in 2016. By 2030 the pipeline export levels are 94-78 bcm. Total Norwegian gas production peaks between 2015 and 2020, with peak production at 124-135 bcm/year. By 2030 the production is 96-115 bcm/year. The results show that there is a limited potential for increased gas exports from Norway to the EU and that Norwegian gas production is declining by 2030 in all scenarios. Annual Norwegian pipeline gas exports to the EU, by 2030, may even be 20 bcm lower than today's level.

  9. An innovative model for regulating supplement products: Natural health products in Canada

    International Nuclear Information System (INIS)

    Nestmann, Earle R.; Harwood, Melody; Martyres, Stephanie

    2006-01-01

    On 1 January 2004, Health Canada officially added a new term to the global list of synonyms for dietary supplements: natural health products (NHP). Developed with the intent of providing Canadian consumers with ready access to NHP that are safe, effective, and of high quality, the Natural Health Products Regulations (the NHP regulations) are applicable to the sale, manufacture, packaging, labelling, importation, distribution, and storage of NHP, and are administered by the recently formed Natural Health Products Directorate (NHPD) within Health Canada. This paper provides an overview of the process for regulating supplement products in Canada

  10. NATURAL AND ARTIFICIAL RADIOACTIVITY IN IMPORTED FISHERY PRODUCTS

    Directory of Open Access Journals (Sweden)

    R. Cavallina

    2013-02-01

    Full Text Available Considering the growing public concern over marine environmental quality, a program of monitoring radionuclids in imported fishery products has been established. The analyses have been performed by gamma spectrometry allowing the rapid detection of many artificial radionuclides such as 137Cs, 134Cs and 40K. The data obtained show Cs radionuclides levels within expected limits. The detection of natural radionuclide K showed different levels in the same fishery product as they came from different sea areas.

  11. Idaho Habitat and Natural Production Monitoring : Annual Report 1989.

    Energy Technology Data Exchange (ETDEWEB)

    Kiefer, Russell B.; Forster, Katharine A.

    1991-01-01

    Project 83-7 was established under the Northwest Power Planning Council's 1982 Fish and Wildlife Program to monitor natural production of anadromous fish, evaluate Bonneville Power Administration (BPA) habitat improvement projects, and develop a credit record for off-site mitigation projects in Idaho. Project 83-7 is divided into two subprojects: general and intensive monitoring. Primary objectives of the general monitoring subproject (Part 1) are to determine natural production increases due to habitat improvement projects in terms of parr production and to determine natural production status and trends in Idaho. The second objective is accomplished by combining parr density data from monitoring and evaluation of BPA habitat projects and from other Idaho Department of Fish and Game (IDFG) management and research activities. Primary objectives of the intensive monitoring subproject (Part 2) are to determine the number of returning chinook and steelhead adults necessary to achieve optimal smolt production and to develop mitigation accounting based on increases in smolt production. Two locations are being intensively studied to meet these objectives. Field work began in 1987 in the upper Salmon River and Crooked River (South Fork Clearwater River tributary). 22 refs., 10 figs., 17 tabs.

  12. Natural products as storage media for avulsed tooth

    Directory of Open Access Journals (Sweden)

    Deepika Jain

    2015-01-01

    Full Text Available Avulsion of tooth is complete displacement of tooth out of its socket that results in mutilation of periodontal ligaments. The desirable treatment option is replantation of the tooth. However, unsuccessful replantation is a matter of great discontentment. Unsuccessful replantation is due to inappropriate management of the avulsed tooth. Protection of teeth from desiccation due to drying of the periodontal ligament tissue, by keeping it in storage media can improve the outcome of the treatment. This review paper focuses on the use of natural products as storage media for avulsed teeth. In vitro and in vivo research published during 1995-2014, allowing open access on National Center for Biotechnology Information (NCBI database and articles on EBSCO host (EBSCO-Elton B. Stephens Company were included. It was found that natural products such as milk, coconut water, propolis, green tea, red mulberry, Aloe vera, egg-white and pomegranate have shown ability to maintain viability of periodontal ligament cells of avulsed teeth. Few natural products such as coconut water and milk can be used in raw form, while other products such as green tea and red mulberry need processing. Ability to maintain periodontal cell viability for a longer time is warranted in cases of major accidents, where teeth can be replanted only after other major surgeries. Natural products have easy availability, greater efficacy and longer storage time as compared to Hank′s balanced salt solution which has been recommended by the International Association of Dental Traumatology as standard solution for storage of avulsed teeth. Natural products have shown good qualities in in vitro experiments; further in vivo studies are needed to evaluate their efficacy as storage media.

  13. Antioxidant Activity of Natural Products against Aluminium Fluoride ...

    African Journals Online (AJOL)

    Evaluation of antioxidant potential of natural products against Aluminium fluoride (AlF4) induced oxidative stress in albino mice were represented in the present study. Gossypin, Quercetin dehydrate, (-)-Epicatechin gallate, Gallic acid and Suramin sulphate (G-protein inhibitor) were evaluated for antioxidant activity by ...

  14. Applications of natural products in the control of mosquito ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-12-29

    Dec 29, 2009 ... there are many alternatives to synthetic, chemical pesticides that await discovery and development. Although some natural products have ... indices for insecticide evaluation: namely, the LD50 and the test period, respectively. ... Biological control of mosquito larvae has been mana- ged through the use of ...

  15. Natural products chemistry research: progress in China in 2011.

    Science.gov (United States)

    Ye, Yang; Li, Xi-Qiang; Tang, Chun-Ping; Yao, Sheng

    2013-03-01

    This article reviews the progress made by Chinese scientists in the field of natural products chemistry in 2011. Selected compounds with unique structural features and/or promising bioactivities are described herein on the basis of structural types. Copyright © 2013 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  16. Natural products chemistry research 2010's progress in China.

    Science.gov (United States)

    Ye, Yang; Li, Xi-Qiang; Tang, Chun-Ping; Yao, Sheng

    2012-01-01

    This article reviews the progresses made by Chinese scientists in the field of natural products chemistry in 2010. Selected compounds with unique structural features and/or promising bioactivities were described herein on the basis of structural types. Copyright © 2012 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  17. Chemistry of natural products: A veritable approach to the ...

    African Journals Online (AJOL)

    Even with the advent of newer technologies such as combinatorial chemistry, robotics, high throughput screening (HTS), bioinformatics, and in silico molecular modelling, natural products still play a crucial role in drug discovery. This is because they provide an unparalleled range of chemical diversity on which the newer ...

  18. Natural Products Research in China from 2015 to 2016

    Science.gov (United States)

    Liu, Haishan; Zhu, Guoliang; Fan, Yaqin; Du, Yuqi; Lan, Mengmeng; Xu, Yibo; Zhu, Weiming

    2018-03-01

    This review covers the literature published by Chinese chemists from 2015 to 2016 on natural products (NPs), with 1,985 citations referring to 6,944 new compounds isolated from marine or terrestrial microorganisms, plants, and animals. The emphasis is on 730 new compounds with a novel skeleton or/and significant bioactivity, together with their source organism and country of origin.

  19. TR Seshadri's Contributions to the Chemistry of Natural Products ...

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 9; Issue 2. T. R. Seshadri's Contributions to the Chemistry of Natural Products - Some Illustrative Examples. N R Krishnaswamy. General Article Volume 9 Issue 2 February 2004 pp 26-38 ...

  20. Low Carbon Technology Options for the Natural Gas Electricity Production

    Science.gov (United States)

    The ultimate goal of this task is to perform environmental and economic analysis of natural gas based power production technologies (different routes) to investigate and evaluate strategies for reducing emissions from the power sector. It is a broad research area. Initially, the...

  1. Natural Products as Anti- HIV Agents | Adesegun | Nigerian ...

    African Journals Online (AJOL)

    The present report gives a concise account of the efforts of Traditional practitioners and herbal scientists or Pharmacognocists at unraveling promising natural products of terrestrial (plants) and marine origins with activity against the HIV. A wide range of plants and marine organism's families and origins, containing various ...

  2. Expedient total synthesis of pyrrothine natural products and analogs

    DEFF Research Database (Denmark)

    Hjelmgaard, Thomas; Givskov, Michael Christian; Nielsen, John

    2007-01-01

    This paper describes an expedient and straightforward total synthesis of the two pyrrothine natural products holomycin (7 steps, 11% overall) and xenorhabdin I (7 steps, 11% overall) and analogs thereof via a common late-stage intermediate. The pathway proceeds via the pyrrothine hydrochloride...

  3. Plant natural products research in tuberculosis drug discovery and ...

    African Journals Online (AJOL)

    The global resurgence of TB and the development of multidrug-resistant tuberculosis (MDR TB) and extensively drug-resistant tuberculosis (XDR-TB), call for the development of new anti-tuberculosis drugs to combat this disease. Plant natural products have a proven global history of treating diseases and ailments.

  4. Genomes to natural products PRediction Informatics for Secondary Metabolomes (PRISM)

    Science.gov (United States)

    Skinnider, Michael A.; Dejong, Chris A.; Rees, Philip N.; Johnston, Chad W.; Li, Haoxin; Webster, Andrew L. H.; Wyatt, Morgan A.; Magarvey, Nathan A.

    2015-01-01

    Microbial natural products are an invaluable source of evolved bioactive small molecules and pharmaceutical agents. Next-generation and metagenomic sequencing indicates untapped genomic potential, yet high rediscovery rates of known metabolites increasingly frustrate conventional natural product screening programs. New methods to connect biosynthetic gene clusters to novel chemical scaffolds are therefore critical to enable the targeted discovery of genetically encoded natural products. Here, we present PRISM, a computational resource for the identification of biosynthetic gene clusters, prediction of genetically encoded nonribosomal peptides and type I and II polyketides, and bio- and cheminformatic dereplication of known natural products. PRISM implements novel algorithms which render it uniquely capable of predicting type II polyketides, deoxygenated sugars, and starter units, making it a comprehensive genome-guided chemical structure prediction engine. A library of 57 tailoring reactions is leveraged for combinatorial scaffold library generation when multiple potential substrates are consistent with biosynthetic logic. We compare the accuracy of PRISM to existing genomic analysis platforms. PRISM is an open-source, user-friendly web application available at http://magarveylab.ca/prism/. PMID:26442528

  5. Evaluation of natural products as possible alternatives to methyl ...

    African Journals Online (AJOL)

    Evaluation of natural products as possible alternatives to methyl bromide in soil fumigation. ... We present the results of the inhibitory activity of crude extracts from some Kenyan medicinal plants against the soil pathogens, Fusarium oxysporum, Alternaria passiflorae, and Aspergillus niger. Crude organic extracts from ...

  6. Liquefied natural gas production at Hammerfest: A transforming marine community

    NARCIS (Netherlands)

    Bets, van L.K.J.; Tatenhove, van J.P.M.; Mol, A.P.J.

    2016-01-01

    Global energy demand and scarce petroleum resources require communities to adapt to a rapidly changing Arctic environment, but as well to a transforming socio-economic environment instigated by oil and gas development. This is illustrated by liquefied natural gas production by Statoil at Hammerfest,

  7. Natural resins and bioactive natural products thereof as potential antimicrobial agents.

    Science.gov (United States)

    Termentzi, Aikaterini; Fokialakis, Nikolas; Skaltsounis, Alexios Leandros

    2011-01-01

    Natural products and their derivatives have historically been invaluable as a source of therapeutic agents and have contributed to the discovery of antimicrobial agents. However, today with the development of drug-resistant strains, new scaffolds and new sources of bioactive compounds are needed. To this end, plant derived natural resins are reviewed for their potential application as antimicrobial agents. Natural gums, extracts of the whole resins, as well as specific extracts, fractions, essential oils and isolated compounds from the above resins are discussed in terms of their antifungal, antibacterial, and antiprotozoal activity.

  8. Natural product derived insecticides: discovery and development of spinetoram.

    Science.gov (United States)

    Galm, Ute; Sparks, Thomas C

    2016-03-01

    This review highlights the importance of natural product research and industrial microbiology for product development in the agricultural industry, based on examples from Dow AgroSciences. It provides an overview of the discovery and development of spinetoram, a semisynthetic insecticide derived by a combination of a genetic block in a specific O-methylation of the rhamnose moiety of spinosad coupled with neural network-based QSAR and synthetic chemistry. It also emphasizes the key role that new technologies and multidisciplinary approaches play in the development of current spinetoram production strains.

  9. Gamma radiodecontamination of natural products uses in Cuban pharmaceutical industry

    International Nuclear Information System (INIS)

    Rodriguez, M.; Lopez, M.; Guerra, M.; Lastra, H.; Prieto, E.; Padron, E.

    1997-01-01

    The aim of the present paper was to carry out the gamma radiodecontamination of industrial productions from there medicinal plant species (C. officinalis, P. incarta and M. recuttia) and two pharmaceuticals forms (S. platensis and bee pollen tabs) which presented high levels of microbiological count. Adequate irradiation doses calculated for each product were used in decontamination. The results obtained showed the effectiveness of the process in the elimination of microbial contamination from theses natural products. No changes in nutritional constituents or physico-chemical properties were observed

  10. Recent advances in screening of natural products for antimicrobial agents.

    Science.gov (United States)

    Zhou, Meng; Luo, Hao; Li, Zhi; Wu, Feng; Huang, Canhua; Ding, Zhenyu; Li, Rui

    2012-05-01

    It has been a very long history for human to resist diseases. During this period, a large number of drugs that could kill or inhibit the growth of microbe has been discovered, most of which were natural products. However, there may still be a large amount of antimicrobial medicines in natural compounds which have not been found yet. The ways of screening for antimicrobial always cost a long time and need a lot of manpower before. However, in recent years, a lot of new antimicrobial targets, antimicrobial drugs and screening methods which are simpler, faster and more efficient have been invented. In this paper the newly discovered targets, natural products and representative technologies were reviewed, which were expected to make some contributions to the research and development of medicines.

  11. Synthesis of New Anti-Cancer Natural Products

    DEFF Research Database (Denmark)

    Yu, Wanwan

    2016-01-01

    this class of natural products of great importance in efforts to synthesize and investigate the masked mechanism responsible for the hypoxia selective cytotoxicity, which have so far led to two synthetic development of rakicidin A, including one from our lab in early 2016. With synthetic entry into rakicidin...... given by the inhibitory effects on the HIF-1 transcriptional pathway (STR-2 (1), STR-3 (2), STR-8 (3)), and cancer cell invasion inhibition (STR-8 (3), STR-26 (4)). The mechanistic explanations of these interactions between cancer cell cellular actions and the small natural molecules of the STR family...... have yet to be deduced. This class of marine natural products of meroterpenoids could contribute to the realm of the hypoxia-related cancer research. The complex meroterpenoid framework, stereochemical demands and bicyclic δ-lactone moiety found in most of the bioactive STR molecules, make...

  12. Mitosis-targeting natural products for cancer prevention and therapy.

    Science.gov (United States)

    Rao, Chinthalapally V; Kurkjian, Carla D; Yamada, Hiroshi Y

    2012-12-01

    Mitosis is a complex process resulting in division of a cell into two daughter cells, and its failure often results in the death of the daughter cells (via apoptotic, necrotic, or proliferative/senescent death). Many chemicals that inhibit the mitotic process (anti-mitotic drugs) have proven effective for killing cancer cells in vitro and in clinical settings. Among the most studied anti-mitotic drugs are plant-origin natural products including taxanes (e.g. paclitaxel, docetaxel) and vinca alkaloids (e.g. vincristine, vinblastine), whose validated target is the spindle microtubules. With the success of these agents, efforts have been made to develop other spindle poisons as well as to improve efficacy of existing spindle poisons with structural modifications. Novel drugs and natural products that inhibit other proteins involved in mitosis (nonmicrotubule targets) have been sought in hopes of expanding available cancer-directed therapies. Recently, significant advances have been made in the understanding of mitotic mechanisms in tumor cells as well as in normal epithelial cells. These advances help us to identify and develop potential natural agents for the prevention and treatment of cancer. This review will focus on natural products that target mitotic process and/or proteins involved in mitotic progression.

  13. Natural radioactivity and estimated dose in Brazilian tobacco products

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Aline S.G.R. de; Damatto, Sandra R., E-mail: aline.oliveira@ipen.br, E-mail: damatto@ipen.br [Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), São Paulo, SP (Brazil)

    2017-07-01

    Tobacco products contain significant concentrations of natural radionuclides from {sup 238}U and {sup 232}Th series. The consumption of these products increases the internal dose of radiation due to the inhalation of the natural radionuclides. Studies from literature emphasize that tobacco products have measurable concentrations of {sup 210}Po and {sup 210}Pb, and may contribute significantly to the increase of internal radiation dose and a large number of lung cancer in smokers. The objectives of this work were to determine the concentrations (Bq/kg) of the radionuclides {sup 226}Ra, {sup 228}Ra, {sup 210}Pb and {sup 210}Po and calculate the internal doses of radiation due to the consumption of these products. In the present work 71 samples were analyzed, consisting of cigars, unflavored and flavored cigarettes, straw cigarettes, cigars and roll smoke. The samples were purchased in Brazilian popular commercial establishments. The analytical techniques employed were the gross alpha and beta measurement after radiochemical separation for the radionuclides {sup 226}Ra, {sup 228}Ra, {sup 210}Pb and alpha spectrometry for {sup 210}Po. The internal radiation doses were calculated with the activity concentrations determined and using the ICRP Publication 119 dose coefficients. An annual consumption of 3,650 kg of tobacco products was considered. The inhalation rates of each radionuclide followed the rates of the current literature. The estimated mean annual dose varied from 76 to 263μSv/y for the tobacco product studied in this work. (author)

  14. Natural radioactivity and estimated dose in Brazilian tobacco products

    International Nuclear Information System (INIS)

    Oliveira, Aline S.G.R. de; Damatto, Sandra R.

    2017-01-01

    Tobacco products contain significant concentrations of natural radionuclides from 238 U and 232 Th series. The consumption of these products increases the internal dose of radiation due to the inhalation of the natural radionuclides. Studies from literature emphasize that tobacco products have measurable concentrations of 210 Po and 210 Pb, and may contribute significantly to the increase of internal radiation dose and a large number of lung cancer in smokers. The objectives of this work were to determine the concentrations (Bq/kg) of the radionuclides 226 Ra, 228 Ra, 210 Pb and 210 Po and calculate the internal doses of radiation due to the consumption of these products. In the present work 71 samples were analyzed, consisting of cigars, unflavored and flavored cigarettes, straw cigarettes, cigars and roll smoke. The samples were purchased in Brazilian popular commercial establishments. The analytical techniques employed were the gross alpha and beta measurement after radiochemical separation for the radionuclides 226 Ra, 228 Ra, 210 Pb and alpha spectrometry for 210 Po. The internal radiation doses were calculated with the activity concentrations determined and using the ICRP Publication 119 dose coefficients. An annual consumption of 3,650 kg of tobacco products was considered. The inhalation rates of each radionuclide followed the rates of the current literature. The estimated mean annual dose varied from 76 to 263μSv/y for the tobacco product studied in this work. (author)

  15. Interdisciplinary researches for potential developments of drugs and natural products

    Directory of Open Access Journals (Sweden)

    Arunrat Chaveerach

    2017-04-01

    Full Text Available Developments of drugs or natural products from plants are possibly made, simple to use and lower cost than modern drugs. The development processes can be started with studying local wisdom and literature reviews to choose the plants which have long been used in diverse areas, such as foods, traditional medicine, fragrances and seasonings. Then those data will be associated with scientific researches, namely plant collection and identification, phytochemical screening by gas chromatography-mass spectrometry, pharmacological study/review for their functions, and finally safety and efficiency tests in human. For safety testing, in vitro cell toxicity by cell viability assessment and in vitro testing of DNA breaks by the comet assay in human peripheral blood mononuclear cells can be performed. When active chemicals and functions containing plants were chosen with safety and efficacy for human uses, then, the potential medicinal natural products will be produced. Based on these procedures, the producing cost will be cheaper and the products can be evaluated for their clinical properties. Thus, the best and lowest-priced medicines and natural products can be distributed worldwide.

  16. Conformation-activity relationships of polyketide natural products.

    Science.gov (United States)

    Larsen, Erik M; Wilson, Matthew R; Taylor, Richard E

    2015-08-01

    Polyketides represent an important class of secondary metabolites that interact with biological targets connected to a variety of disease-associated pathways. Remarkably, nature's assembly lines, polyketide synthases, manufacture these privileged structures through a combinatorial mixture of just a few structural units. This review highlights the role of these structural elements in shaping a polyketide's conformational preferences, the use of computer-based molecular modeling and solution NMR studies in the identification of low-energy conformers, and the importance of conformational analogues in probing the bound conformation. In particular, this review covers several examples wherein conformational analysis complements classic structure-activity relationships in the design of biologically active natural product analogues.

  17. Natural product for the treatment of Alzheimer's disease.

    Science.gov (United States)

    Bui, Thanh Tung; Nguyen, Thanh Hai

    2017-09-26

    Alzheimer's disease (AD) is related to increasing age. It is mainly characterized by progressive neurodegenerative disease, which damages memory and cognitive function. Natural products offer many options to reduce the progress and symptoms of many kinds of diseases, including AD. Meanwhile, natural compound structures, including lignans, flavonoids, tannins, polyphenols, triterpenes, sterols, and alkaloids, have anti-inflammatory, antioxidant, anti-amyloidogenic, and anticholinesterase activities. In this review, we summarize the pathogenesis and targets for treatment of AD. We also present several medicinal plants and isolated compounds that are used for preventing and reducing symptoms of AD.

  18. Conceptual process synthesis for recovery of natural products from plants

    DEFF Research Database (Denmark)

    Malwade, Chandrakant R.; Qu, Haiyan; Rong, Ben-Guang

    2013-01-01

    A systematic method of conceptual process synthesis for recovery of natural products from their biological sources is presented. This methodology divides the task into two major subtasks namely, isolation of target compound from a chemically complex solid matrix of biological source (crude extract......) and purification of target compound(s) from the crude extract. Process analytical technology (PAT) is used in each step to understand the nature of material systems and separation characteristics of each separation method. In the present work, this methodology is applied to generate process flow sheet for recovery...

  19. Tandem Biocatalysis Unlocks the Challenging de Novo Production of Plant Natural Products.

    Science.gov (United States)

    Duplais, Christophe; Estevez, Yannick

    2017-11-16

    Intimate partnership: Knowledge of the biocatalytic cascades in different cellular compartments is limited, but deciphering these systems in nature can be used to inspire synthetic strategies. Two studies report new insights into the biosynthesis of alkaloids and sesterterpenoids in plants. This highlight presents these novel biotransformations to illustrate how tandem biocatalysis can impact the future of natural product production. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. The Assessment of Alumina Production Waste Impact on Natural Water

    Directory of Open Access Journals (Sweden)

    Vladimir Sergeevich Kuznetsov

    2018-03-01

    Full Text Available The paper is dedicated to the issue of assessment of alumina (red mud production waste on natural water. The growth of the number of aluminium-producing facilities leads to the expansion of exclusion areas to store the production waste – sludge dumps. A considerable part of research on red mud utilisation is focused on its use in the iron-and-steel industry. Furthermore, the technologies of red mud usage in the construction industry gain substantial significance for land reclamation, isolation of polluted industrial and agricultural lands as well as the effluent and industrial emissions treatment.

  1. Strain Prioritization and Genome Mining for Enediyne Natural Products

    Science.gov (United States)

    Yan, Xiaohui; Ge, Huiming; Huang, Tingting; Hindra; Yang, Dong; Teng, Qihui; Crnovčić, Ivana; Li, Xiuling; Rudolf, Jeffrey D.; Lohman, Jeremy R.; Gansemans, Yannick; Zhu, Xiangcheng; Huang, Yong; Zhao, Li-Xing; Jiang, Yi; Van Nieuwerburgh, Filip; Rader, Christoph

    2016-01-01

    ABSTRACT The enediyne family of natural products has had a profound impact on modern chemistry, biology, and medicine, and yet only 11 enediynes have been structurally characterized to date. Here we report a genome survey of 3,400 actinomycetes, identifying 81 strains that harbor genes encoding the enediyne polyketide synthase cassettes that could be grouped into 28 distinct clades based on phylogenetic analysis. Genome sequencing of 31 representative strains confirmed that each clade harbors a distinct enediyne biosynthetic gene cluster. A genome neighborhood network allows prediction of new structural features and biosynthetic insights that could be exploited for enediyne discovery. We confirmed one clade as new C-1027 producers, with a significantly higher C-1027 titer than the original producer, and discovered a new family of enediyne natural products, the tiancimycins (TNMs), that exhibit potent cytotoxicity against a broad spectrum of cancer cell lines. Our results demonstrate the feasibility of rapid discovery of new enediynes from a large strain collection. PMID:27999165

  2. Metal-Catalyzed Asymmetric Michael Addition in Natural Product Synthesis.

    Science.gov (United States)

    Hui, Chunngai; Pu, Fan; Xu, Jing

    2017-03-23

    Asymmetric catalysis for chiral compound synthesis is a rapidly growing field in modern organic chemistry. Asymmetric catalytic processes have been indispensable for the synthesis of enantioselective materials to meet demands from various fields. Michael addition has been used extensively for the construction of C-C bonds under mild conditions. With the discovery and development of organo- and metal-catalyzed asymmetric Michael additions, the synthesis of enantioselective and/or diastereoselective Michael adducts has become possible and increasingly prevalent in the literature. In particular, metal-catalyzed asymmetric Michael addition has been employed as a key reaction in natural product synthesis for the construction of contiguous quaternary stereogenic center(s), which is still a difficult task in organic synthesis. Previously reported applications of metal-catalyzed asymmetric Michael additions in natural product synthesis are presented here and discussed in depth. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. What Is the Structure of the Antitubercular Natural Product Eucapsitrione?

    Science.gov (United States)

    Pullella, Glenn A; Wild, Duncan A; Nealon, Gareth L; Elyashberg, Mikhail; Piggott, Matthew J

    2017-07-21

    1,5,7-Trihydroxy-6H-indeno[1,2-b]anthracene-6,11,13-trione (1), proposed to be the antitubercular natural product eucapsitrione, has been synthesized in 43% overall yield and six steps, including a key Suzuki-Miyaura biaryl coupling and a directed remote metalation (DReM)-initiated cyclization. The physical and spectroscopic properties of 1 do not match the data reported for the natural product. At this time there is insufficient information available to enable a structure reassignment. During the optimization of the Suzuki-Miyaura coupling, an unprecedented biaryl coupling ortho to the borono group was observed. The scope of this unusual reaction has been investigated.

  4. Natural product antifoulants from the octocorals of Indian waters

    Digital Repository Service at National Institute of Oceanography (India)

    Raveendran, T.V.; LimnaMol, V.P.; Parameswaran, P.S.

    1 Author version: International Biodeterioration & Biodegradation, vol.65(1); 2011; 265-268 Natural Product Antifoulants from the Octocorals of Indian waters T.V. Raveendran * , V.P. Limna Mol, P.S. Parameswaran National Institute... bioactive metabolites with potential biomedical applications. For example, eleutherobin, isolated from soft coral Eleutherobia sp., exhibited anticancer properties (Ata et al., 2004); the diterpene,13-Epi-9-deacetoxy-xenicinin, isolated from the soft...

  5. Cytotoxic Natural Products from Marine Sponge-Derived Microorganisms.

    Science.gov (United States)

    Zhang, Huawei; Zhao, Ziping; Wang, Hong

    2017-03-10

    A growing body of evidence indicates that marine sponge-derived microbes possess the potential ability to make prolific natural products with therapeutic effects. This review for the first time provides a comprehensive overview of new cytotoxic agents from these marine microbes over the last 62 years from 1955 to 2016, which are assorted into seven types: terpenes, alkaloids, peptides, aromatics, lactones, steroids, and miscellaneous compounds.

  6. Bioactive Natural Products of Marine Sponges from the Genus Hyrtios

    OpenAIRE

    Nourhan Hisham Shady; Ebaa M. El-Hossary; Mostafa A. Fouad; Tobias A. M. Gulder; Mohamed Salah Kamel; Usama Ramadan Abdelmohsen

    2017-01-01

    Marine sponges are known as a rich source for novel bioactive compounds with valuable pharmacological potential. One of the most predominant sponge genera is Hyrtios, reported to have various species such as Hyrtios erectus, Hyrtios reticulatus, Hyrtios gumminae, Hyrtios communis, and Hyrtios tubulatus and a number of undescribed species. Members of the genus Hyrtios are a rich source of natural products with diverse and valuable biological activities, represented by different chemical classe...

  7. Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies

    OpenAIRE

    Tanaka, Hiroyuki; Putalun, Waraporn; Shoyama, Yukihiro

    2012-01-01

    We succeeded in developing the fingerprint of natural product by eastern blotting using monoclonal antibodies. After developing and separating them on a TLC plate, solasodine glycosides are oxidized by NaIO4 and reacted with a protein to give conjugates which are recognized with anti-solamargine monoclonal antibody (MAb). Anti-solamargine MAb having wide cross-reactivity can stain and detect all solasodine glycosides by fingerprint. Different sensitivity between solamargine and solasonine was...

  8. Natural Products for the Treatment of Type 2 Diabetes Mellitus.

    Science.gov (United States)

    Ríos, José Luis; Francini, Flavio; Schinella, Guillermo R

    2015-08-01

    Type 2 diabetes mellitus is a metabolic disease characterized by persistent hyperglycemia. High blood sugar can produce long-term complications such as cardiovascular and renal disorders, retinopathy, and poor blood flow. Its development can be prevented or delayed in people with impaired glucose tolerance by implementing lifestyle changes or the use of therapeutic agents. Some of these drugs have been obtained from plants or have a microbial origin, such as galegine isolated from Galega officinalis, which has a great similarity to the antidiabetic drug metformin. Picnogenol, acarbose, miglitol, and voglibose are other antidiabetic products of natural origin. This review compiles the principal articles on medicinal plants used for treating diabetes and its comorbidities, as well as mechanisms of natural products as antidiabetic agents. Inhibition of α-glucosidase and α-amylase, effects on glucose uptake and glucose transporters, modification of mechanisms mediated by the peroxisome proliferator-activated receptor, inhibition of protein tyrosine phosphatase 1B activity, modification of gene expression, and activities of hormones involved in glucose homeostasis such as adiponectin, resistin, and incretin, and reduction of oxidative stress are some of the mechanisms in which natural products are involved. We also review the most relevant clinical trials performed with medicinal plants and natural products such as aloe, banaba, bitter melon, caper, cinnamon, cocoa, coffee, fenugreek, garlic, guava, gymnema, nettle, sage, soybean, green and black tea, turmeric, walnut, and yerba mate. Compounds of high interest as potential antidiabetics are: fukugetin, palmatine, berberine, honokiol, amorfrutins, trigonelline, gymnemic acids, gurmarin, and phlorizin. Georg Thieme Verlag KG Stuttgart · New York.

  9. Natural Products as Source of Potential Dengue Antivirals

    Directory of Open Access Journals (Sweden)

    Róbson Ricardo Teixeira

    2014-06-01

    Full Text Available Dengue is a neglected disease responsible for 22,000 deaths each year in areas where it is endemic. To date, there is no clinically approved dengue vaccine or antiviral for human beings, even though there have been great efforts to accomplish these goals. Several approaches have been used in the search for dengue antivirals such as screening of compounds against dengue virus enzymes and structure-based computational discovery. During the last decades, researchers have turned their attention to nature, trying to identify compounds that can be used as dengue antivirals. Nature represents a vast reservoir of substances that can be explored with the aim of discovering new leads that can be either used directly as pharmaceuticals or can serve as lead structures that can be optimized towards the development of new antiviral agents against dengue. In this review we describe an assortment of natural products that have been reported as possessing dengue antiviral activity. The natural products are organized into classes of substances. When appropriate, structure-activity relationships are outlined. The biological assays used to assess antiviral activity are briefly described.

  10. Natural products research in South Africa: 1890–2010

    Directory of Open Access Journals (Sweden)

    Siegfried E. Drewes

    2012-05-01

    Full Text Available Having spent some 50 years as an organic chemist with an interest in medicinal plant chemistry in South Africa it was relevant now to ask three questions, (1 when were natural products first utilised, (2 who were the people involved, and (3 what is the status quo? Based on older literature published in the South African Journal of Chemistry, information gleaned from attendance at innumerable chemistry conferences, and relevant literature in university archives, a great deal of information was gathered to answer the first two questions. For example, that the first veterinarian to treat cattle diseases caused by poisonous plants in the Eastern Cape was Dr Jotella Soga in the 1890s. Contributions from other prominent scientists such as Marais, Rindl, Rimington and Warren followed. From about 1940 to the 1990s, researchers concentrated mainly on the isolation of new compounds from local plants for which some indigenous knowledge was recorded. Foreign chemists also arrived and did a fair amount of ‘exploitation’ of natural products. Thus, the anti-cancer compound combretastatin was first isolated from the indigenous tree Combretum caffrum. Plant chemistry in South Africa has blossomed in the last decade, with many students from previously disadvantaged backgrounds, but with a keen interest in muti or medicinal chemistry, entering the field. Recent findings have rekindled the belief that a major development in natural products would at last emerge from Africa.

  11. An overview on the potential of natural products as ureas

    Directory of Open Access Journals (Sweden)

    Luzia V. Modolo

    2015-01-01

    Full Text Available Ureases, enzymes that catalyze urea hydrolysis, have received considerable attention for their impact on living organisms’ health and life quality. On the one hand, the persistence of urease activity in human and animal cells can be the cause of some diseases and pathogen infections. On the other hand, food production can be negatively affected by ureases of soil microbiota that, in turn, lead to losses of nitrogenous nutrients in fields supplemented with urea as fertilizer. In this context, nature has proven to be a rich resource of natural products bearing a variety of scaffolds that decrease the ureolytic activity of ureases from different organisms. Therefore, this work compiles the state-of-the-art researches focused on the potential of plant natural products (present in extracts or as pure compounds as urease inhibitors of clinical and/or agricultural interests. Emphasis is given to ureases of Helicobacter pylori, Canavalia ensiformis and soil microbiota although the active site of this class of hydrolases is conserved among living organisms.

  12. Screening a Natural Product-Based Library against Kinetoplastid Parasites.

    Science.gov (United States)

    Zulfiqar, Bilal; Jones, Amy J; Sykes, Melissa L; Shelper, Todd B; Davis, Rohan A; Avery, Vicky M

    2017-10-12

    Kinetoplastid parasites cause vector-borne parasitic diseases including leishmaniasis, human African trypanosomiasis (HAT) and Chagas disease. These Neglected Tropical Diseases (NTDs) impact on some of the world's lowest socioeconomic communities. Current treatments for these diseases cause severe toxicity and have limited efficacy, highlighting the need to identify new treatments. In this study, the Davis open access natural product-based library was screened against kinetoplastids ( Leishmania donovani DD8, Trypanosoma brucei brucei and Trypanosoma cruzi ) using phenotypic assays. The aim of this study was to identify hit compounds, with a focus on improved efficacy, selectivity and potential to target several kinetoplastid parasites. The IC 50 values of the natural products were obtained for L. donovani DD8, T. b. brucei and T. cruzi in addition to cytotoxicity against the mammalian cell lines, HEK-293, 3T3 and THP-1 cell lines were determined to ascertain parasite selectivity. Thirty-one compounds were identified with IC 50 values of ≤ 10 µM against the kinetoplastid parasites tested. Lissoclinotoxin E ( 1 ) was the only compound identified with activity across all three investigated parasites, exhibiting IC 50 values natural products with the potential to be new chemical starting points for drug discovery efforts for kinetoplastid diseases were identified.

  13. Natural Radiation in byproducts of the production of phosphoric acid

    International Nuclear Information System (INIS)

    Silveira, Marcilei A. Guazzelli da; Cardoso, L.L.; Medina, N.H.

    2014-01-01

    Natural radiation is the largest source of radiation exposure to which man is subject. It is formed basically by cosmic radiation and the radionuclides present in the Earth crust, as 40 K and the elements of the decay series of 232 Th and 238 U. Phosphate ores, which constitutes the raw material for the production of phosphoric acid, have a high rate of natural radiation from the decay series of 232 Th and 238 U. Phosphogypsum, which is naturally radioactivity, is a by-product of the production of phosphoric acid by the wet method. For each ton of phosphoric acid it is produced about 4.5 tons of phosphogypsum. This work presents the analysis of samples collected in all stages of the manufacturing process of phosphoric acid, which generates the phosphogypsum. Gamma-ray spectrometry was used to measure the concentration of the elements of the decay series of 232 Th and 238 U. All analyzed samples showed a high concentration of radionuclides, promoting the need for further steps in the process in order to reduce the presence of such radionuclides in the phosphogypsum. The results indicate the radionuclide 238 U has higher contribution in some samples of the intermediate stages of the process. All samples exceeded the international average range of human exposure to terrestrial gamma radiation, which is 0.3 to 1.0 mSv/year. (author)

  14. Antimycobacterial natural products--an opportunity for the Colombian biodiversity.

    Science.gov (United States)

    Bueno, Juan; Coy, Ericsson David; Stashenko, Elena

    2011-12-01

    It is estimated that one-third part of the world population is infected with the tubercle bacillus. While only a small percentage of infected individuals will develop clinical tuberculosis, each year there are approximately eight million new cases and two million deaths. Mycobacterium tuberculosis is thus responsible for more human mortality than any other single microbial species. The goals of tuberculosis control are focused to cure active disease, prevent relapse, reduce transmission and avert the emergence of drug-resistance. For over 50 years, natural products have served us well on combating infectious bacteria and fungi. During the 20th century, microbial and plant secondary metabolites have helped to double our life span, reduced pain and suffering, and revolutionized medicine. Colombia is a megadiverse country with enormous potential to offer leads for new antimycobacterial drugs. The principal aim of this article is to show a state of the art on antimycobacterial natural products research in Colombia compared to the rest of the world, in order to develop programs for bioprospecting with a view to determining the biological activity for pharmaceutical and industrial application of natural products in our country.

  15. Bioactive natural products with anti-herpes simplex virus properties.

    Science.gov (United States)

    Hassan, Sherif T S; Masarčíková, Radka; Berchová, Kateřina

    2015-10-01

    In this review, we highlight and summarise the most promising extracts, fractions and pure compounds as potential anti-herpes simplex virus (HSV) agents derived from microorganisms, marine organisms, fungi, animals and plants. The role of natural products in the development of anti-HSV drugs will be discussed. Herpes simplex viruses (HSV-1 and -2) are common human pathogens that remain a serious threat to human health. In recent years, a great interest has been devoted to the search for integrated management of HSV infections. Acyclovir and related nucleoside analogues have been licensed for the therapy that target viral DNA polymerase. Although these drugs are currently effective against HSV infections, the intensive use of these drugs has led to the problem of drug-resistant strains. Therefore, the search for new sources to develop new antiherpetic agents has gained major priority to overcome the problem. Natural products as potential, new anti-HSV drugs provide several advantages such as reduced side effects, less resistance, low toxicity and various mechanisms of action. This paper aims to provide an overview of natural products that possess antiviral activity against HSV. © 2015 Royal Pharmaceutical Society.

  16. Marine natural products in prevention and treatment of osteoporosis

    Directory of Open Access Journals (Sweden)

    Zahra Ghanbari

    2015-05-01

    Full Text Available Undoubtedly, pharmaceutical and nutritional factors play an important role in the prevention of age-related bone loss. According to the several studies so far, the effects of nutrients and bioactive components which are extracted from marine resources are very promising in osteoporosis. Most of these investigations have been done on various marine algae extracts. Since, algae are rich source of essential minerals, primary and secondary unique natural products, several amino acids and growth factors their extracts show favorable effects on bone metabolism. Moreover, it has been shown that marine nutrients such as marine fishes, shrimp and crabs increase the absorption of calcium and bone collagen synthesis or reduce the production of prostaglandins and decrease the deoxypyridinoline disposal. On the other hand, secondary products which are extracted and characterized from marine organisms such as mollusks, fungi, bacteria, sponges and coral reefs show anti-osteoporosis activities via the inhibition of osteoclast differentiation and the induction of apoptosis in osteoclasts like cells or stimulation of osteoblast differentiation. Although, several investigations have been done in this area, many of studies have been carried out on animal models, like ovariectomy-induced bone loss in mice. Hence, clinical investigations are warranted to develop marine natural products against bone loss and to prevent osteoporosis.

  17. The water-food nexus of natural rubber production

    Science.gov (United States)

    Chiarelli, D. D.; Rosa, L.; Rulli, M. C.; D'Odorico, P.

    2017-12-01

    The increasing global demand for natural rubber (100% increase in the last 15 years) is for most part met by Malaysia and Indonesia, and - to a lesser extent - other countries in south-east Asia and Africa. The consequent expansion of rubber plantation has often occurred at the expenses of agricultural land for staple food, particularly in southeast Asia, where most of the land suitable for agriculture is already harvested for food crops or other uses. Here we investigate the extent to which the ongoing increase in rubber production is competing with the food system and affecting the livelihoods of rural communities in the areas of production and their appropriation of natural resources, such as water. We also investigate to what extent the expansion of rubber plantations is taking place through large scale land acquisitions (LSLAs) and evaluate the impacts on rural communities. Our results show how rubber production has strong environmental, social and economic impacts. Despite their ability to bring employment and increase the average income of economically disadvantaged areas, rubber plantations may threaten the local water and food security and induce a loss of rural livelihoods, particularly when the new plantations result from LSLAs that displace semi-subsistence forms of production thereby forcing the local populations to depend on global markets.

  18. Biocombinatorial Engineering of Fungal PKS-NRPS Hybrids for Production of Novel Synthetic Natural Products

    DEFF Research Database (Denmark)

    Nielsen, Maria Lund

    Natural products have had a huge impact on the history of drug development, and today, these compounds continue to be a key source of novel drug leads. Over the years, natural products have been exploited for their pharmacological and biological activities in the treatment of many diseases....... Secondary metabolites (SMs) from bacteria, plants and filamentous fungi constitute a large group of important natural products. In this thesis I explore the biosynthesis of several fungal SMs along with the enzymes responsible for their synthesis. Specifically, my research focuses on the expression...... and engineering of a certain type of fungal enzymes – natural fusions of polyketide synthases and nonribosomal peptide synthetases (PKS-NRPSs). The thesis is divided into two topics: 1) Expanding fungal chemodiversity through combinatorial biosynthesis 2) Two CRISPR-Cas9-based approaches to linking SMs...

  19. Metabolomics tools for the synthetic biology of natural products.

    Science.gov (United States)

    Hollywood, Katherine A; Schmidt, Kamila; Takano, Eriko; Breitling, Rainer

    2018-03-19

    Metabolomics plays an increasingly central role within the Design-Build-Test cycle of synthetic biology, in particular in applications targeting the discovery, diversification and optimised production of a wide range of natural products. For example, improved methods for the online monitoring of chemical reactions accelerate data generation to be compatible with the rapid iterations and increasing library sizes of automated synthetic biology pipelines. Combinations of label-free metabolic profiling and 13 C-based flux analysis lead to increased resolution in the identification of metabolic bottlenecks affecting product yield in engineered microbes. And molecular networking strategies drastically increase our ability to identify and characterise novel chemically complex biomolecules of interest in a diverse range of samples. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. Pseudomonas putida-a versatile host for the production of natural products.

    Science.gov (United States)

    Loeschcke, Anita; Thies, Stephan

    2015-08-01

    The biosynthesis of natural products by heterologous expression of biosynthetic pathways in amenable production strains enables biotechnological access to a variety of valuable compounds by conversion of renewable resources. Pseudomonas putida has emerged as a microbial laboratory work horse, with elaborated techniques for cultivation and genetic manipulation available. Beyond that, this bacterium offers several particular advantages with regard to natural product biosynthesis, notably a versatile intrinsic metabolism with diverse enzymatic capacities as well as an outstanding tolerance to xenobiotics. Therefore, it has been applied for recombinant biosynthesis of several valuable natural products. This review provides an overview of applications of P. putida as a host organism for the recombinant biosynthesis of such natural products, including rhamnolipids, terpenoids, polyketides and non-ribosomal peptides, and other amino acid-derived compounds. The focus is on de novo natural product synthesis from intrinsic building blocks by means of heterologous gene expression and strain engineering. Finally, the future potential of the bacterium as a chassis organism for synthetic microbiology is pointed out.

  1. Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources.

    Science.gov (United States)

    Ni, Jun; Tao, Fei; Du, Huaiqing; Xu, Ping

    2015-09-02

    Plant secondary metabolites have been attracting people's attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using microbial genes, a new metabolic route was developed in E. coli for the synthesis of vanillin, the most widely used flavoring agent. A series of factors were systematically investigated for raising production, including efficiency and suitability of genes, gene dosage, and culture media. The metabolically engineered strain produced 97.2 mg/L vanillin from l-tyrosine, 19.3 mg/L from glucose, 13.3 mg/L from xylose and 24.7 mg/L from glycerol. These results show that the metabolic route enables production of natural vanillin from low-cost substrates, suggesting that it is a good strategy to mimick natural pathways for artificial pathway design.

  2. Mimicking a natural pathway for de novo biosynthesis: natural vanillin production from accessible carbon sources

    Science.gov (United States)

    Ni, Jun; Tao, Fei; Du, Huaiqing; Xu, Ping

    2015-01-01

    Plant secondary metabolites have been attracting people’s attention for centuries, due to their potentials; however, their production is still difficult and costly. The rich diversity of microbes and microbial genome sequence data provide unprecedented gene resources that enable to develop efficient artificial pathways in microorganisms. Here, by mimicking a natural pathway of plants using microbial genes, a new metabolic route was developed in E. coli for the synthesis of vanillin, the most widely used flavoring agent. A series of factors were systematically investigated for raising production, including efficiency and suitability of genes, gene dosage, and culture media. The metabolically engineered strain produced 97.2 mg/L vanillin from l-tyrosine, 19.3 mg/L from glucose, 13.3 mg/L from xylose and 24.7 mg/L from glycerol. These results show that the metabolic route enables production of natural vanillin from low-cost substrates, suggesting that it is a good strategy to mimick natural pathways for artificial pathway design. PMID:26329726

  3. Natural products as potential cancer therapy enhancers: A preclinical update

    Directory of Open Access Journals (Sweden)

    Abed Agbarya

    2014-09-01

    Full Text Available Cancer is a multifactorial disease that arises as a consequence of alterations in many physiological processes. Recently, hallmarks of cancer were suggested that include sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative immortality, inducing angiogenesis, and activating invasion and metastasis, along with two emerging hallmarks including reprogramming energy metabolism and escaping immune destruction. Treating multifactorial diseases, such as cancer with agents targeting a single target, might provide partial treatment and, in many cases, disappointing cure rates. Epidemiological studies have consistently shown that the regular consumption of fruits and vegetables is strongly associated with a reduced risk of developing chronic diseases, such as cardiovascular diseases and cancer. Since ancient times, plants, herbs, and other natural products have been used as healing agents. Moreover, the majority of the medicinal substances available today have their origin in natural compounds. Traditionally, pharmaceuticals are used to cure diseases, and nutrition and herbs are used to prevent disease and to provide an optimal balance of macro- and micro-nutrients needed for good health. We explored the combination of natural products, dietary nutrition, and cancer chemotherapeutics for improving the efficacy of cancer chemotherapeutics and negating side effects.

  4. Production of bio-synthetic natural gas in Canada.

    Science.gov (United States)

    Hacatoglu, Kevork; McLellan, P James; Layzell, David B

    2010-03-15

    Large-scale production of renewable synthetic natural gas from biomass (bioSNG) in Canada was assessed for its ability to mitigate energy security and climate change risks. The land area within 100 km of Canada's network of natural gas pipelines was estimated to be capable of producing 67-210 Mt of dry lignocellulosic biomass per year with minimal adverse impacts on food and fiber production. Biomass gasification and subsequent methanation and upgrading were estimated to yield 16,000-61,000 Mm(3) of pipeline-quality gas (equivalent to 16-63% of Canada's current gas use). Life-cycle greenhouse gas emissions of bioSNG-based electricity were calculated to be only 8.2-10% of the emissions from coal-fired power. Although predicted production costs ($17-21 GJ(-1)) were much higher than current energy prices, a value for low-carbon energy would narrow the price differential. A bioSNG sector could infuse Canada's rural economy with $41-130 billion of investments and create 410,000-1,300,000 jobs while developing a nation-wide low-carbon energy system.

  5. (3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.

    Science.gov (United States)

    Paterna, Angela; Gomes, Sofia E; Borralho, Pedro M; Mulhovo, Silva; Rodrigues, Cecília M P; Ferreira, Maria-José U

    2016-12-24

    Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. Through fractionation, (3'R)-hydroxytaberanelegantine C (1), a vobasinyl-iboga bisindole alkaloid, was isolated from a cytotoxic alkaloid fraction of the methanol extract of T. elegans roots. Its structure was identified by spectroscopic methods, mainly 1D and 2D NMR experiments. Compound 1 was evaluated for its ability to induce apoptosis in HCT116 and SW620 colon and HepG2 liver carcinoma cells. The cell viability of compound 1 was evaluated by the MTS and lactate dehydrogenase (LDH) assays. Induction of apoptosis was analyzed through Guava ViaCount assay, by flow cytometry, caspase-3/7 activity assays and evaluation of nuclear morphology by Hoechst staining. To determine the molecular pathways elicited by 1 exposure, immunoblot analysis was also performed. (3'R)-hydroxytaberanelegantine C (1) displayed strong apoptosis induction activity as compared to 5-fluorouracil (5-FU), the most used anticancer agent in colorectal cancer treatment. In the MTS assay, compound 1 exhibited IC 50 values similar or lower than 5-FU in the three cell lines tested. The IC 50 value of 1 was also calculated in CCD18co normal human colon fibroblasts. The lactate dehydrogenase assay showed increased LDH release by compound 1, and the Guava ViaCount assay revealed that 1 significantly increased the incidence of apoptosis to a further extent than 5-FU. Moreover, the induction of apoptosis was corroborated by evaluation of nuclear morphology by Hoechst staining and caspase-3/7 activity assays of 1 treated cells. As expected, in immunoblot analysis, compound 1 treatment led to poly(ADP-ribose) polymerase cleavage. This was accompanied by decreased anti-apoptotic proteins Bcl-2 and XIAP steady state levels in all three cancer

  6. Water for wood products versus nature, food or feed

    Science.gov (United States)

    Schyns, Joep; Booij, Martijn; Hoekstra, Arjen

    2017-04-01

    more water available for the generation of other ecosystem services. Our findings contribute to a more complete picture of the human appropriation of water and the understanding of the interlinkages between the SDGs, thus feeding the debate on water for wood products versus nature, food or feed.

  7. Green extraction of natural products: concept and principles.

    Science.gov (United States)

    Chemat, Farid; Vian, Maryline Abert; Cravotto, Giancarlo

    2012-01-01

    The design of green and sustainable extraction methods of natural products is currently a hot research topic in the multidisciplinary area of applied chemistry, biology and technology. Herein we aimed to introduce the six principles of green-extraction, describing a multifaceted strategy to apply this concept at research and industrial level. The mainstay of this working protocol are new and innovative technologies, process intensification, agro-solvents and energy saving. The concept, principles and examples of green extraction here discussed, offer an updated glimpse of the huge technological effort that is being made and the diverse applications that are being developed.

  8. Bioactive Natural Products of Marine Sponges from the Genus Hyrtios

    Directory of Open Access Journals (Sweden)

    Nourhan Hisham Shady

    2017-05-01

    Full Text Available Marine sponges are known as a rich source for novel bioactive compounds with valuable pharmacological potential. One of the most predominant sponge genera is Hyrtios, reported to have various species such as Hyrtios erectus, Hyrtios reticulatus, Hyrtios gumminae, Hyrtios communis, and Hyrtios tubulatus and a number of undescribed species. Members of the genus Hyrtios are a rich source of natural products with diverse and valuable biological activities, represented by different chemical classes including alkaloids, sesterterpenes and sesquiterpenes. This review covers the literature until June 2016, providing a complete survey of all compounds isolated from the genus Hyrtios with their corresponding biological activities whenever applicable.

  9. Bioactive Natural Products from Animal Associated-Microbes.

    Science.gov (United States)

    Tian, Yuan; Li, Yan-Ling; Zhao, Feng-Chun

    2017-01-01

    Animal associated-microbes are miroorganisms living inside animal hosts during some parts of their life. In view of the special environment, it is considered that the unique microbes might be the producer of new compounds with diversity biological activities. This review summarizes new findings (mainly described since 2011) concerning the characteristics of various natural products that can be extracted from animal associated-microbes, highlighting that animal related microorganisms represent an underexplored reservoir for the discovery of molecules with unique scaffolds and promising biological activities. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  10. The Canadian Natural Health Products (NHP Regulations: Industry Compliance Motivations

    Directory of Open Access Journals (Sweden)

    Hina Laeeque

    2007-01-01

    Full Text Available This qualitative study explores corporations' motivations to comply with new natural health products (NHP Regulations in Canada. Interviews were conducted with representatives from 20 Canadian NHP companies. Findings show that the rationale for compliance differs for large compared to small and medium-sized enterprises (SMEs. Large firms are motivated to comply with the regulations because of the deterrent fear of negative media coverage, social motivations, ability to comply and maintaining a competitive market advantage. In contrast, SMEs are motivated to comply due to the deterrent fear of legal prosecution and a sense of duty.

  11. Natural product diversity of actinobacteria in the Atacama Desert.

    Science.gov (United States)

    Rateb, Mostafa E; Ebel, Rainer; Jaspars, Marcel

    2018-02-14

    The Atacama Desert of northern Chile is considered one of the most arid and extreme environment on Earth. Its core region was described as featuring "Mars-like" soils that were at one point deemed too extreme for life to exist. However, recent investigations confirmed the presence of diverse culturable actinobacteria. In the current review, we discuss a total of 46 natural products isolated to date representing diverse chemical classes characterized from different actinobacteria isolated from various locations in the Atacama Desert. Their reported biological activities are also discussed.

  12. A general enantioselective route to the chamigrene natural product family

    KAUST Repository

    White, David E.

    2010-06-01

    Described in this report is an enantioselective route toward the chamigrene natural product family. The key disconnections in our synthetic approach include sequential enantioselective decarboxylative allylation and ring-closing olefin metathesis to form the all-carbon quaternary stereocenter and spirocyclic core present in all members of this class of compounds. The generality of this strategy is demonstrated by the first total syntheses of elatol and the proposed structure of laurencenone B, as well as the first enantioselective total syntheses of laurencenone C and α-chamigrene. A brief exploration of the substrate scope of the enantioselective decarboxylative allylation/ring-closing metathesis sequence with fully substituted vinyl chlorides is also presented.

  13. Production of mullite from natural raw materials using mechanical activation

    International Nuclear Information System (INIS)

    Feitosa, G.; Pinheiro, D.G.; Chinelatto, A.L.; Chinelatto, A.S.A

    2009-01-01

    Mullite, ceramic structure consisting by alumina and silica, is present in different ceramic industry, as refractory, white ceramic, titles and advanced ceramics. This is because this material presents high resistance mechanical and high resistance in high temperatures, beyond low expansion coefficients and thermal conductivity. On the other hand, the mullite production needs high temperatures. This work had as objective the production of mullite from natural raw materials, using mechanical activation. Bauxite and quartz had been mixed in the stoichiometric composition and the mixture was submitted to high energy milling for different times. Then non milled powders and milled powders had been sintered in different temperatures. The characterization was made by X Ray diffraction and scanning electronic microscopy. The results demonstrated that the high energy milling affects the mullite formation, decreasing the temperature in that it is formed. (author)

  14. Marine Vibrionaceae as a source of bioactive natural products

    DEFF Research Database (Denmark)

    Månsson, Maria; Wietz, Matthias; Gram, Lone

    Vibrionaceae are Gram-negative bacteria found widespread in the marine environment where they are particularly abundant on the surface of marine macroorganisms. Production of antibacterial compounds appears to be common among vibrios, yet vibrios are largely underexplored for their proclivity...... to produce secondary metabolites. We have studied the production of antibacterial compounds in Vibrionaceae collected during a global marine expedition, Galathea 3. Apart from growth inhibitory compounds, we searched for compounds that interfere with virulence regulation in Staphylococcus aureus. We found...... that some strains were capable of producing antibacterial compounds when grown on natural substrates such as chitin or seaweed. One Vibrio coralliilyticus strain was capable of producing the antibacterial compound when using chitin as the sole carbon source and in a live chitin model system, suggesting...

  15. Personal-Care Products Formulated with Natural Antioxidant Extracts

    Directory of Open Access Journals (Sweden)

    Maria Luisa Soto

    2018-01-01

    Full Text Available The objective of this study was to evaluate the potential use of some vegetal raw materials in personal-care products. Four ethanolic extracts (grape pomace, Pinus pinaster wood chips, Acacia dealbata flowers, and Lentinus edodes were prepared and total phenolics, monomeric sugars, and antioxidant capacity were determined on alcoholic extracts. Six of the most important groups of cosmetics products (hand cream, body oil, shampoo, clay mask, body exfoliating cream, and skin cleanser were formulated. Participants evaluated some sensory attributes and overall acceptance by a 10-point scale; the results showed differences among age-intervals, but not between males and females. The results confirmed that all extracts presented characteristics appropriate for their use in cosmetic formulations and their good acceptability by consumers into all cosmetic products. Texture/appearance, spreadability, and skin feeling are important attributes among consumer expectations, but odor and color were the primary drivers and helped differentiate the natural extracts added into all personal-care products.

  16. US production of natural gas from tight reservoirs

    International Nuclear Information System (INIS)

    1993-01-01

    For the purposes of this report, tight gas reservoirs are defined as those that meet the Federal Energy Regulatory Commission's (FERC) definition of tight. They are generally characterized by an average reservoir rock permeability to gas of 0.1 millidarcy or less and, absent artificial stimulation of production, by production rates that do not exceed 5 barrels of oil per day and certain specified daily volumes of gas which increase with the depth of the reservoir. All of the statistics presented in this report pertain to wells that have been classified, from 1978 through 1991, as tight according to the FERC; i.e., they are ''legally tight'' reservoirs. Additional production from ''geologically tight'' reservoirs that have not been classified tight according to the FERC rules has been excluded. This category includes all producing wells drilled into legally designated tight gas reservoirs prior to 1978 and all producing wells drilled into physically tight gas reservoirs that have not been designated legally tight. Therefore, all gas production referenced herein is eligible for the Section 29 tax credit. Although the qualification period for the credit expired at the end of 1992, wells that were spudded (began to be drilled) between 1978 and May 1988, and from November 5, 1990, through year end 1992, are eligible for the tax credit for a subsequent period of 10 years. This report updates the EIA's tight gas production information through 1991 and considers further the history and effect on tight gas production of the Federal Government's regulatory and tax policy actions. It also provides some high points of the geologic background needed to understand the nature and location of low-permeability reservoirs

  17. Applications of natural zeolites on agriculture and food production.

    Science.gov (United States)

    Eroglu, Nazife; Emekci, Mevlut; Athanassiou, Christos G

    2017-08-01

    Zeolites are crystalline hydrated aluminosilicates with remarkable physical and chemical properties, which include losing and receiving water in a reverse way, adsorbing molecules that act as molecular sieves, and replacing their constituent cations without structural change. The commercial production of natural zeolites has accelerated during the last 50 years. The Structure Commission of the International Zeolite Association recorded more than 200 zeolites, which currently include more than 40 naturally occurring zeolites. Recent findings have supported their role in stored-pest management as inert dust applications, pesticide and fertilizer carriers, soil amendments, animal feed additives, mycotoxin binders and food packaging materials. There are many advantages of inert dust application, including low cost, non-neurotoxic action, low mammalian toxicity and safety for human consumption. The latest consumer trends and government protocols have shifted toward organic origin materials to replace synthetic chemical products. In the present review, we summarize most of the main uses of zeolites in food and agruculture, along with the with specific paradigms that illustrate their important role. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  18. Total Synthesis of Natural Products Using Hypervalent Iodine Reagents

    Directory of Open Access Journals (Sweden)

    Gaetan eMaertens

    2015-01-01

    Full Text Available We present a review of natural product syntheses accomplished in our laboratory during the last five years. Each synthetic route features a phenol dearomatization promoted by an environmentally benign hypervalent iodine reagent. The dearomatizations demonstrate the aromatic ring umpolung concept, and involve stereoselective remodeling of the inert unsaturations of a phenol into a highly functionalized key intermediate that may contain a quaternary carbon center and a prochiral dienone system. Several new oxidative strategies were employed, including transpositions (1,3-alkyl shift and Prins-pinacol, a polycyclization, an ipso rearrangement, and direct nucleophilic additions at the phenol para position. Several alkaloids, heterocyclic compounds, and a polycyclic core have been achieved, including sceletenone (a serotonin reuptake inhibitor, acetylaspidoalbidine (an antitumor agent, fortucine (antiviral and antitumor, erysotramidine (curare-like effect, platensimycin (an antibiotic, and the main core of a kaurane diterpene (immunosuppressive agent and stimulator of apoptosis. These concise and in some cases enantioselective syntheses effectively demonstrate the importance of hypervalent iodine reagents in the total synthesis of bioactive natural products.

  19. The potential of natural products for targeting PPARα

    Directory of Open Access Journals (Sweden)

    Daniela Rigano

    2017-07-01

    Full Text Available Peroxisome proliferator activated receptors (PPARs α, -γ and -β/δ are ligand-activated transcription factors and members of the superfamily of nuclear hormone receptor. These receptors play key roles in maintaining glucose and lipid homeostasis by modulating gene expression. PPARs constitute a recognized druggable target and indeed several classes of drugs used in the treatment of metabolic disease symptoms, such as dyslipidemia (fibrates, e.g. fenofibrate and gemfibrozil and diabetes (thiazolidinediones, e.g. rosiglitazone and pioglitazone are ligands for the various PPAR isoforms. More precisely, antidiabetic thiazolidinediones act on PPARγ, while PPARα is the main molecular target of antidyslipidemic fibrates. Over the past few years, our understanding of the mechanism underlying the PPAR modulation of gene expression has greatly increased. This review presents a survey on terrestrial and marine natural products modulating the PPARα system with the objective of highlighting how the incredible chemodiversity of natural products can provide innovative leads for this “hot” target.

  20. A diversity oriented synthesis of natural product inspired molecular libraries.

    Science.gov (United States)

    Chauhan, Jyoti; Luthra, Tania; Gundla, Rambabu; Ferraro, Antonio; Holzgrabe, Ulrike; Sen, Subhabrata

    2017-11-07

    Natural products are the source of innumerable pharmaceutical drug candidates and also form an important aspect of herbal remedies. They are also a source of various bioactive compounds. Herein we have leveraged the structural attributes of several natural products in building a library of architecturally diverse chiral molecules by harnessing R-tryptophan as the chiral auxiliary. It is converted to its corresponding methyl ester 1 which in turn provided a bevy of 1-aryl-tetrahydro-β-carbolines 2a-d, which were then converted to chiral compounds via a diversity oriented synthetic strategy (DOS). In general, intermolecular and intramolecular ring rearrangements facilitated the formation of the final compounds. Four different classes of molecules with distinct architectures were generated, adding up to nearly twenty-two individual molecules. Phenotypic screening of a representative section of the library revealed two molecules that selectively inhibit MCF7 breast cancer cells with IC 50 of ∼5 μg mL -1 potency.

  1. Systems biology approaches to understand natural products biosynthesis

    Directory of Open Access Journals (Sweden)

    Cuauhtemoc eLicona-Cassani

    2015-12-01

    Full Text Available Actinomycetes populate soils and aquatic sediments which impose biotic and abiotic challenges for their survival. As a result, actinomycetes metabolism and genomes have evolved to produce an overwhelming diversity of specialized molecules. Polyketides, non-ribosomal peptides, post-translationally modified peptides, lactams and terpenes are well known bioactive natural products with enormous industrial potential. Accessing such biological diversity has proven difficult due to the complex regulation of cellular metabolism in actinomycetes and to the sparse knowledge of their physiology. The past decade, however, has seen the development of omics technologies that have significantly contributed to our better understanding of their biology. Key observations have contributed towards a shift in the exploitation of actinomycetes biology, such as using their full genomic potential, activating entire pathways through key metabolic elicitors and pathway engineering to improve biosynthesis. Here, we review recent efforts devoted to achieving enhanced discovery, activation and manipulation of natural product biosynthetic pathways in model actinomycetes using genome-scale biological datasets.

  2. Experience curve for natural gas production by hydraulic fracturing

    International Nuclear Information System (INIS)

    Fukui, Rokuhei; Greenfield, Carl; Pogue, Katie; Zwaan, Bob van der

    2017-01-01

    From 2007 to 2012 shale gas production in the US expanded at an astounding average growth rate of over 50%/yr, and thereby increased nearly tenfold over this short time period alone. Hydraulic fracturing technology, or “fracking”, as well as new directional drilling techniques, played key roles in this shale gas revolution, by allowing for extraction of natural gas from previously unviable shale resources. Although hydraulic fracturing technology had been around for decades, it only recently became commercially attractive for large-scale implementation. As the production of shale gas rapidly increased in the US over the past decade, the wellhead price of natural gas dropped substantially. In this paper we express the relationship between wellhead price and cumulative natural gas output in terms of an experience curve, and obtain a learning rate of 13% for the industry using hydraulic fracturing technology. This learning rate represents a measure for the know-how and skills accumulated thus far by the US shale gas industry. The use of experience curves for renewable energy options such as solar and wind power has allowed analysts, practitioners, and policy makers to assess potential price reductions, and underlying cost decreases, for these technologies in the future. The reasons for price reductions of hydraulic fracturing are fundamentally different from those behind renewable energy technologies – hence they cannot be directly compared – and hydraulic fracturing may soon reach, or maybe has already attained, a lower bound for further price reductions, for instance as a result of its water requirements or environmental footprint. Yet, understanding learning-by-doing phenomena as expressed by an industry-wide experience curve for shale gas production can be useful for strategic planning in the gas sector, as well as assist environmental policy design, and serve more broadly as input for projections of energy system developments. - Highlights: • Hydraulic

  3. Lichen symbiosis: nature's high yielding machines for induced hydrogen production.

    Directory of Open Access Journals (Sweden)

    Aikaterini Papazi

    Full Text Available Hydrogen is a promising future energy source. Although the ability of green algae to produce hydrogen has long been recognized (since 1939 and several biotechnological applications have been attempted, the greatest obstacle, being the O2-sensitivity of the hydrogenase enzyme, has not yet been overcome. In the present contribution, 75 years after the first report on algal hydrogen production, taking advantage of a natural mechanism of oxygen balance, we demonstrate high hydrogen yields by lichens. Lichens have been selected as the ideal organisms in nature for hydrogen production, since they consist of a mycobiont and a photobiont in symbiosis. It has been hypothesized that the mycobiont's and photobiont's consumption of oxygen (increase of COX and AOX proteins of mitochondrial respiratory pathways and PTOX protein of chrolorespiration establishes the required anoxic conditions for the activation of the phycobiont's hydrogenase in a closed system. Our results clearly supported the above hypothesis, showing that lichens have the ability to activate appropriate bioenergetic pathways depending on the specific incubation conditions. Under light conditions, they successfully use the PSII-dependent and the PSII-independent pathways (decrease of D1 protein and parallel increase of PSaA protein to transfer electrons to hydrogenase, while under dark conditions, lichens use the PFOR enzyme and the dark fermentative pathway to supply electrons to hydrogenase. These advantages of lichen symbiosis in combination with their ability to survive in extreme environments (while in a dry state constitute them as unique and valuable hydrogen producing natural factories and pave the way for future biotechnological applications.

  4. The Antitumor Potential of Marine Natural Products: A Mechanistic Investigation.

    Science.gov (United States)

    Hu, Liping; Ying, Jie; Zhang, Miaomiao; Qiu, Xiaoyan; Lu, Yu

    2017-09-18

    Compounds obtained from natural resources have been the important candidates for the discovery and development of drugs. Over the past few decades, marine resources gradually attracted the attention of the majority of researchers, and a number of compounds with various structures or activities have been obtained from sponge, alga, fungus, mollusks and some others. Because of the living conditions in the ocean are higher pressures, more variable temperatures, lower oxygen and lower light than that in the terrestrial environment, marine resources have a lot of organisms that are not available or rare on land. It is expected to find agents exhibited good activity for diseases treatment from marine organisms, especially anti-tumor activity. Among the marine compounds obtained, most of them have the good antitumor activities, and some have been used for clinical treatment of tumors, some are in clinical trials. These natural products through different pathways do their unique antitumor effects to induce cells apoptosis, inhibit cells proliferation and migration. A variety of drugs have been used for the clinical treatment of cancer during the last few years, but for the advanced cancer patients, extending the survival time is not long. And due to the lack of effective drugs to control the transfer of cancer cells and with the development of drug-resistant microbes, researchers are actively looking for active compounds to overcome these problems. Here, we summarized the marine natural products obtained during the past few years, and analyzed their anti-tumor effects and mechanism. This will provide a significant basis for anticancer drugs' screening and development. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  5. Lichen Symbiosis: Nature's High Yielding Machines for Induced Hydrogen Production

    Science.gov (United States)

    Papazi, Aikaterini; Kastanaki, Elizabeth; Pirintsos, Stergios; Kotzabasis, Kiriakos

    2015-01-01

    Hydrogen is a promising future energy source. Although the ability of green algae to produce hydrogen has long been recognized (since 1939) and several biotechnological applications have been attempted, the greatest obstacle, being the O2-sensitivity of the hydrogenase enzyme, has not yet been overcome. In the present contribution, 75 years after the first report on algal hydrogen production, taking advantage of a natural mechanism of oxygen balance, we demonstrate high hydrogen yields by lichens. Lichens have been selected as the ideal organisms in nature for hydrogen production, since they consist of a mycobiont and a photobiont in symbiosis. It has been hypothesized that the mycobiont’s and photobiont’s consumption of oxygen (increase of COX and AOX proteins of mitochondrial respiratory pathways and PTOX protein of chrolorespiration) establishes the required anoxic conditions for the activation of the phycobiont’s hydrogenase in a closed system. Our results clearly supported the above hypothesis, showing that lichens have the ability to activate appropriate bioenergetic pathways depending on the specific incubation conditions. Under light conditions, they successfully use the PSII-dependent and the PSII-independent pathways (decrease of D1 protein and parallel increase of PSaA protein) to transfer electrons to hydrogenase, while under dark conditions, lichens use the PFOR enzyme and the dark fermentative pathway to supply electrons to hydrogenase. These advantages of lichen symbiosis in combination with their ability to survive in extreme environments (while in a dry state) constitute them as unique and valuable hydrogen producing natural factories and pave the way for future biotechnological applications. PMID:25826211

  6. Naturally occurring radionuclides in agricultural products: An overview

    International Nuclear Information System (INIS)

    Hanlon, E.A.

    1994-01-01

    Low levels of naturally occurring radionuclides exist in phosphatic clays, a by-product of phosphatic mining and beneficiation processes. Concerns about these radionuclides entering the human food chain were an immediate research priority before the phosphate clays could be reclaimed for intensive agricultural purposes. Efforts included the assembly of a large body of data from both sons and plants, part of which were produced by the Polk County (Florida) Mined Lands Agricultural Research/Demonstration Project MLAR/DP. Additional detailed studies involving dairy and beef cattle (Bos taurus) were conducted by researchers working with the MLAR/DP. A national symposium was conducted in which data concerning the MLAR/DP work and other research projects also dealing with naturally occurring radionuclides in agriculture could be discussed. The symposium included invited review papers dealing with the identification of radionuclide geological origins, the geochemistry and movement of radionuclides within the environment, mechanisms of plant uptake, entry points into the food chain, and evaluation of dose and risk assessment to the consumer of low levels of radionuclides. The risk to human health of an individual obtaining 0.1 of his or her dietary intake from crops produced on phosphatic clays increased by 1 in 5 x 10 6 /yr above a control individual consuming no food grown on phosphatic clays. Leaf tissues were found to be generally higher than fruit, grain, or root tissues. The natural range in radionuclide content among various food types was greater than the difference in radionuclides content between the same food produced on phosphatic clays vs. natural soils. 19 refs

  7. China's natural gas: Resources, production and its impacts

    International Nuclear Information System (INIS)

    Wang, Jianliang; Feng, Lianyong; Zhao, Lin; Snowden, Simon

    2013-01-01

    In order to achieve energy consumption targets, and subsequently reduce carbon emissions, China is working on energy strategies and policies aimed at actively increasing the consumption of natural gas—the lowest carbon energy of the fossil fuels, and to enhance the proportion of gas in total primary energy consumption. To do this, it is a necessary prerequisite that China must have access to adequate gas resources and production to meet demand. This paper shows that the availability of domestic gas resources are overestimated by China's authorities due to differences in classification and definitions of gas resources/reserves between China and those accepted internationally. Based on official gas resource figures, China's gas production remains low with respect to the projected demand, and will only be 164.6 bcm in 2020, far lower than the 375 bcm of forecast demand. The gap between gas production and demand will reach 210.4 bcm by 2020. Existing plans for the importation of gas and the development of unconventional gas will not close this gap in the next 10 years, and this situation will therefore present a severe challenge to China's gas security, achievement of targets in improving energy consumption structure and reducing carbon emissions. - Highlights: ► We show that available gas resources are overestimated by China's authorities. ► We forecast China's future gas production under different resource scenarios. ► This paper shows that China's gas production will not meet the soaring demand. ► The gap between supply and demand will continue to increase rapidly in future. ► China's gas security will meet a severe challenge because of this increasing gap

  8. ACTIVITY OF NATURAL PRODUCTS AGAINST SOME PHYTOPATHOGENIC FUNGI.

    Science.gov (United States)

    La Torre, A; Caradonia, F; Gianferro, M; Molinu, M G; Battaglia, V

    2014-01-01

    The requirement of environmental protection and food safety is perceived with always major interest by public opinion and it is consistent with European Union legislation on the sustainable use of pesticides (Directive 2009/128/EC). This directive requires member states to promote low pesticide-input, giving priority to non-chemical methods and low risk plant protection products. In order to contribute to the achievement of these objectives antifungal activity of natural substances, characterized by a good toxicological and ecotoxicological profile, was tested. Essential oil of Melaleuca alternifolia, essential oil of Syzygium aromaticum and extract from Mimosa tenuiflora were tested against Alternaria alternata, Botrytis cinerea and Fusarium oxysporum f. sp. lycopersici (races 1 and 2). In vitro tests involved determination of radial growth of the colonies of fungi in the presence of varying concentrations of tested products in agar media and determination of germination percentage in the presence of tested product at various concentrations. The products based on essential oil of M. alternifolia were also tested in vivo on tomato fruits wounded and artificially inoculated with A. alternata or with B. cinerea. The in vitro tests showed the antifungal activity of both essential oils instead the extract from M. tenuiflora exhibited poor antifungal activity and only against A. alternata and B. cinerea. The results on tomato fruits showed inhibition of grey mould and black mould by essential oil of M. alternifolia. The antifungal activity increased with increasing concentrations. In conclusion, the obtained results in the present study showed promising prospects for the utilisation of investigated products to reduce the using of antifungal chemicals and to achieve a more sustainable use of pesticides.

  9. Does Osmotic Stress Affect Natural Product Expression in Fungi?

    Science.gov (United States)

    Overy, David; Correa, Hebelin; Roullier, Catherine; Chi, Wei-Chiung; Pang, Ka-Lai; Rateb, Mostafa; Ebel, Rainer; Shang, Zhuo; Capon, Rob; Bills, Gerald; Kerr, Russell

    2017-08-13

    The discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. An unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawater contributes to enhanced secondary metabolism? To answer this question, a terrestrial isolate of Aspergillus aculeatus was fermented in osmotic and saline stress conditions in parallel across multiple sites. The ex-type strain of A. aculeatus was obtained from three different culture collections. Site-to-site variations in metabolite expression were observed, suggesting that subculturing of the same strain and subtle variations in experimental protocols can have pronounced effects upon metabolite expression. Replicated experiments at individual sites indicated that secondary metabolite production was divergent between osmotic and saline treatments. Titers of some metabolites increased or decreased in response to increasing osmolite (salt or glycerol) concentrations. Furthermore, in some cases, the expression of some secondary metabolites in relation to osmotic and saline stress was attributed to specific sources of the ex-type strains.

  10. Natural sweetening of food products by engineering Lactococcus lactis for glucose production

    NARCIS (Netherlands)

    Pool, Wietske A.; Neves, Ana Rute; Kok, Jan; Santos, Helena; Kuipers, Oscar P.

    We show that sweetening of food products by natural fermentation can be achieved by a combined metabolic engineering and transcriptome analysis approach. A Lactococcus lactis ssp. cremoris strain was constructed in which glucose metabolism was completely disrupted by deletion of the genes coding for

  11. Natural gas productive capacity for the lower 48 states 1985 through 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    This publication presents information on wellhead productive capacity and a projection of gas production requirements. A history of natural gas production and productive capacity at the wellhead, along with a projection of the same, is illustrated.

  12. Delivering natural products and biotherapeutics to improve drug efficacy.

    Science.gov (United States)

    Obeid, Mohammad A; Al Qaraghuli, Mohammed M; Alsaadi, Manal; Alzahrani, Abdullah R; Niwasabutra, Kanidta; Ferro, Valerie A

    2017-11-01

    Due to the increasing problem of drug resistance, new and improved medicines are required. Natural products and biotherapeutics offer a vast resource for new drugs; however, challenges, including the cost and time taken for traditional drug discovery processes and the subsequent lack of investment from the pharmaceutical industry, are associated with these areas. New techniques are producing compounds with appropriate activity at a faster rate. While the formulation of these combined with drug-delivery systems offers a promising approach for expanding the drug developments available to modern medicine. Here, various classes of drug-delivery systems are described and the advantages they bring to small molecule and biotherapeutic targeting are highlighted. This is an attractive approach to the pharmaceutical industry and the rising trend in research in this area is examined in brief. [Formula: see text].

  13. Inhibitors of Microglial Neurotoxicity: Focus on Natural Products

    Directory of Open Access Journals (Sweden)

    Kyoungho Suk

    2011-01-01

    Full Text Available Microglial cells play a dual role in the central nervous system as they have both neurotoxic and neuroprotective effects. Uncontrolled and excessive activation of microglia often contributes to inflammation-mediated neurodegeneration. Recently, much attention has been paid to therapeutic strategies aimed at inhibiting neurotoxic microglial activation. Pharmacological inhibitors of microglial activation are emerging as a result of such endeavors. In this review, natural products-based inhibitors of microglial activation will be reviewed. Potential neuroprotective activity of these compounds will also be discussed. Future works should focus on the discovery of novel drug targets that specifically mediate microglial neurotoxicity rather than neuroprotection. Development of new drugs based on these targets may require a better understanding of microglial biology and neuroinflammation at the molecular, cellular, and systems levels.

  14. Can Invalid Bioactives Undermine Natural Product-Based Drug Discovery?

    Science.gov (United States)

    2015-01-01

    High-throughput biology has contributed a wealth of data on chemicals, including natural products (NPs). Recently, attention was drawn to certain, predominantly synthetic, compounds that are responsible for disproportionate percentages of hits but are false actives. Spurious bioassay interference led to their designation as pan-assay interference compounds (PAINS). NPs lack comparable scrutiny, which this study aims to rectify. Systematic mining of 80+ years of the phytochemistry and biology literature, using the NAPRALERT database, revealed that only 39 compounds represent the NPs most reported by occurrence, activity, and distinct activity. Over 50% are not explained by phenomena known for synthetic libraries, and all had manifold ascribed bioactivities, designating them as invalid metabolic panaceas (IMPs). Cumulative distributions of ∼200,000 NPs uncovered that NP research follows power-law characteristics typical for behavioral phenomena. Projection into occurrence–bioactivity–effort space produces the hyperbolic black hole of NPs, where IMPs populate the high-effort base. PMID:26505758

  15. Marine Natural Products from New Caledonia—A Review

    Directory of Open Access Journals (Sweden)

    Sofia-Eléna Motuhi

    2016-03-01

    Full Text Available Marine micro- and macroorganisms are well known to produce metabolites with high biotechnological potential. Nearly 40 years of systematic prospecting all around the New Caledonia archipelago and several successive research programs have uncovered new chemical leads from benthic and planktonic organisms. After species identification, biological and/or pharmaceutical analyses are performed on marine organisms to assess their bioactivities. A total of 3582 genera, 1107 families and 9372 species have been surveyed and more than 350 novel molecular structures have been identified. Along with their bioactivities that hold promise for therapeutic applications, most of these molecules are also potentially useful for cosmetics and food biotechnology. This review highlights the tremendous marine diversity in New Caledonia, and offers an outline of the vast possibilities for natural products, especially in the interest of pursuing collaborative fundamental research programs and developing local biotechnology programs.

  16. Biosynthesis and chemical synthesis of presilphiperfolanol natural products.

    Science.gov (United States)

    Hong, Allen Y; Stoltz, Brian M

    2014-05-19

    Presilphiperfolanols constitute a family of biosynthetically important sesquiterpenes which can rearrange to diverse sesquiterpenoid skeletons. While the origin of these natural products can be traced to simple linear terpene precursors, the details of the enzymatic cyclization mechanism that forms the stereochemically dense tricyclic skeleton has required extensive biochemical, computational, and synthetic investigation. Parallel efforts to prepare the unique and intriguing structures of these compounds by total synthesis have also inspired novel strategies, thus resulting in four synthetic approaches and two completed syntheses. While the biosynthesis and chemical synthesis studies performed to date have provided much insight into the role and properties of these molecules, emerging questions regarding the biosynthesis of newer members of the family and subtle details of rearrangement mechanisms have yet to be explored. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Influenza neuraminidase: a druggable target for natural products.

    Science.gov (United States)

    Grienke, Ulrike; Schmidtke, Michaela; von Grafenstein, Susanne; Kirchmair, Johannes; Liedl, Klaus R; Rollinger, Judith M

    2012-01-01

    The imminent threat of influenza pandemics and repeatedly reported emergence of new drug-resistant influenza virus strains demonstrate the urgent need for developing innovative and effective antiviral agents for prevention and treatment. At present, influenza neuraminidase (NA), a key enzyme in viral replication, spread, and pathogenesis, is considered to be one of the most promising targets for combating influenza. Despite the substantial medical potential of NA inhibitors (NAIs), only three of these drugs are currently on the market (zanamivir, oseltamivir, and peramivir). Moreover, sudden changes in NAI susceptibility revealed the urgent need in the discovery/identification of novel inhibitors. Nature offers an abundance of biosynthesized compounds comprising chemical scaffolds of high diversity, which present an infinite pool of chemical entities for target-oriented drug discovery in the battle against this highly contagious pathogen. This review illuminates the increasing research efforts of the past decade (2000-2011), focusing on the structure, function and druggability of influenza NA, as well as its inhibition by natural products. Following a critical discussion of publications describing some 150 secondary plant metabolites tested for their inhibitory potential against influenza NA, the impact of three different strategies to identify and develop novel NAIs is presented: (i) bioactivity screening of herbal extracts, (ii) exploitation of empirical knowledge, and (iii) computational approaches. This work addresses the latest developments in theoretical and experimental research on properties of NA that are and will be driving anti-influenza drug development now and in the near future.

  18. Culturable rare Actinomycetes: diversity, isolation and marine natural product discovery.

    Science.gov (United States)

    Subramani, Ramesh; Aalbersberg, William

    2013-11-01

    Rare Actinomycetes from underexplored marine environments are targeted in drug discovery studies due to the Actinomycetes' potentially huge resource of structurally diverse natural products with unusual biological activity. Of all marine bacteria, 10 % are Actinomycetes, which have proven an outstanding and fascinating resource for new and potent bioactive molecules. Past and present efforts in the isolation of rare Actinomycetes from underexplored diverse natural habitats have resulted in the isolation of about 220 rare Actinomycete genera of which more than 50 taxa have been reported to be the producers of 2,500 bioactive compounds. That amount represents greater than 25 % of the total Actinomycetes metabolites, demonstrating that selective isolation methods are being developed and extensively applied. Due to the high rediscovery rate of known compounds from Actinomycetes, a renewed interest in the development of new antimicrobial agents from rare and novel Actinomycetes is urgently required to combat the increasing number of multidrug-resistant human pathogens. To facilitate that discovery, this review updates all selective isolation media including pretreatment and enrichment methods for the isolation of marine rare Actinomycetes. In addition, this review demonstrates that discovering new compounds with novel scaffolds can be increased by intensive efforts in isolating and screening rare marine genera of Actinomycetes. Between 2007 and mid-2013, 80 new rare Actinomycete species were reported from marine habitats. They belong to 23 rare families, of which three are novel, and 20 novel genera. Of them, the family Micromonosporaceae is dominant as a producer of promising chemical diversity.

  19. 77 FR 67398 - Manufacturer of Controlled Substances Notice of Application; National Center For Natural Products...

    Science.gov (United States)

    2012-11-09

    ... DEPARTMENT OF JUSTICE Drug Enforcement Administration Manufacturer of Controlled Substances Notice of Application; National Center For Natural Products Research (NIDA MPROJECT) Pursuant to Sec. 1301..., National Center for Natural Products Research--NIDA MProject, University of Mississippi, 135 Coy Waller...

  20. Natural product-derived drugs for the treatment of inflammatory bowel diseases.

    Science.gov (United States)

    Joo, Young-Eun

    2014-04-01

    Natural products have been used as drugs for millennia, and the therapeutic potential of natural products has been studied for more than a century. Since the mid-1880s, approximately 60% of drugs have originated from natural products. Recently, the importance of using natural products has increased, as has interest in discovering efficient new drugs. Natural drugs are desirable for the treatment of inflammatory bowel diseases, such as ulcerative colitis and Crohn's disease. This review discusses the discovery and development of drugs derived from natural products for the treatment of inflammatory bowel diseases.

  1. Toxic element contamination of natural health products and pharmaceutical preparations.

    Directory of Open Access Journals (Sweden)

    Stephen J Genuis

    Full Text Available BACKGROUND: Concern has recently emerged regarding the safety of natural health products (NHPs-therapies that are increasingly recommended by various health providers, including conventional physicians. Recognizing that most individuals in the Western world now consume vitamins and many take herbal agents, this study endeavored to determine levels of toxic element contamination within a range of NHPs. METHODS: Toxic element testing was performed on 121 NHPs (including Ayurvedic, traditional Chinese, and various marine-source products as well as 49 routinely prescribed pharmaceutical preparations. Testing was also performed on several batches of one prenatal supplement, with multiple samples tested within each batch. Results were compared to existing toxicant regulatory limits. RESULTS: Toxic element contamination was found in many supplements and pharmaceuticals; levels exceeding established limits were only found in a small percentage of the NHPs tested and none of the drugs tested. Some NHPs demonstrated contamination levels above preferred daily endpoints for mercury, cadmium, lead, arsenic or aluminum. NHPs manufactured in China generally had higher levels of mercury and aluminum. CONCLUSIONS: Exposure to toxic elements is occurring regularly as a result of some contaminated NHPs. Best practices for quality control-developed and implemented by the NHP industry with government oversight-is recommended to guard the safety of unsuspecting consumers.

  2. The value of nature's natural product library for the discovery of New Chemical Entities: the discovery of ingenol mebutate.

    Science.gov (United States)

    Ogbourne, Steven M; Parsons, Peter G

    2014-10-01

    In recent decades, 'Big Pharma' has invested billions of dollars into ingenious and innovative strategies designed to develop drugs using high throughput screening of small molecule libraries generated on the laboratory bench. Within the same time frame, screening of natural products by pharmaceutical companies has suffered an equally significant reduction. This is despite the fact that the complexity, functional diversity and druggability of nature's natural product library are considered by many to be superior to any library any team of scientists can prepare. It is therefore no coincidence that the number of New Chemical Entities reaching the market has also suffered a substantial decrease, leading to a productivity crisis within the pharmaceutical sector. In fact, the current dearth of New Chemical Entities reaching the market in recent decades might be a direct consequence of the strategic decision to move away from screening of natural products. Nearly 700 novel drugs derived from natural product New Chemical Entities were approved between 1981 and 2010; more than 60% of all approved drugs over the same time. In this review, we use the example of ingenol mebutate, a natural product identified from Euphorbia peplus and later approved as a therapy for actinic keratosis, as why nature's natural product library remains the most valuable library for discovery of New Chemical Entities and of novel drug candidates. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Production Characteristics of Oceanic Natural Gas Hydrate Reservoirs

    Science.gov (United States)

    Max, M. D.; Johnson, A. H.

    2014-12-01

    Oceanic natural gas hydrate (NGH) accumulations form when natural gas is trapped thermodynamically within the gas hydrate stability zone (GHSZ), which extends downward from the seafloor in open ocean depths greater than about 500 metres. As water depths increase, the thickness of the GHSZ thickens, but economic NGH deposits probably occur no deeper than 1 km below the seafloor. Natural gas (mostly methane) appears to emanate mostly from deeper sources and migrates into the GHSZ. The natural gas crystallizes as NGH when the pressure - temperature conditions within the GHSZ are reached and when the chemical condition of dissolved gas concentration in pore water is high enough to favor crystallization. Although NGH can form in both primary and secondary porosity, the principal economic target appears to be turbidite sands on deep continental margins. Because these are very similar to the hosts of more deeply buried conventional gas and oil deposits, industry knows how to explore for them. Recent improvements in a seismic geotechnical approach to NGH identification and valuation have been confirmed by drilling in the northern Gulf of Mexico and allow for widespread exploration for NGH deposits to begin. NGH concentrations occur in the same semi-consolidated sediments in GHSZs worldwide. This provides for a narrow exploration window with low acoustic attenuation. These sediments present the same range of relatively easy drilling conditions and formation pressures that are only slightly greater than at the seafloor and are essentially equalized by water in wellbores. Expensive conventional drilling equipment is not required. NGH is the only hydrocarbon that is stable at its formation pressures and incapable of converting to gas without artificial stimulation. We suggest that specialized, NGH-specific drilling capability will offer opportunities for much less expensive drilling, more complex wellbore layouts that improve reservoir connectivity and in which gas

  4. Natural product analogues: towards a blueprint for analogue-focused synthesis.

    Science.gov (United States)

    Bebbington, Magnus W P

    2017-08-14

    For the first time a general overview of approaches to the synthesis of natural product analogues is presented. This reflects a process of evolution of natural product synthesis which has accelerated in the years since the implementation of diversity-oriented synthesis, which has emerged in parallel with collective synthesis, diverted total synthesis and the preparation of truncated natural products optimised for biological activity. A method involving computational assessment for the validation of core-modified natural product analogues is discussed.

  5. Recent advances in the elucidation of enzymatic function in natural product biosynthesis.

    Science.gov (United States)

    Tan, Gao-Yi; Deng, Zixin; Liu, Tiangang

    2015-01-01

    With the successful production of artemisinic acid in yeast, the promising potential of synthetic biology for natural product biosynthesis is now being realized. The recent total biosynthesis of opioids in microbes is considered to be another landmark in this field. The importance and significance of enzymes in natural product biosynthetic pathways have been re-emphasized by these advancements. Therefore, the characterization and elucidation of enzymatic function in natural product biosynthesis are undoubtedly fundamental for the development of new drugs and the heterologous biosynthesis of active natural products. Here, discoveries regarding enzymatic function in natural product biosynthesis over the past year are briefly reviewed.

  6. Strategies for the discovery of new natural products by genome mining.

    Science.gov (United States)

    Zerikly, Malek; Challis, Gregory L

    2009-03-02

    Natural products have a very broad spectrum of applications. Many natural products are used clinically as antibacterial, antifungal, antiparasitic, anticancer and immunosuppressive agents and are therefore of utmost importance for our society. When in the 1940s the golden age of antibiotics was ushered in, a "gold rush fever" of natural product discovery in the pharmaceutical industry ensued for many decades. However, the traditional process of discovering new bioactive natural products is generally long and laborious, and known natural products are frequently rediscovered. A mass-withdrawal of pharmaceutical companies from new natural product discovery and natural products research has thus occurred in recent years. In this article, the concept of genome mining for novel natural product discovery, which promises to provide a myriad of new bioactive natural compounds, is summarized and discussed. Genome mining for new natural product discovery exploits the huge and constantly increasing quantity of DNA sequence data from a wide variety of organisms that is accumulating in publicly accessible databases. Genes encoding enzymes likely to be involved in natural product biosynthesis can be readily located in sequenced genomes by use of computational sequence comparison tools. This information can be exploited in a variety of ways in the search for new bioactive natural products.

  7. Effects of bioenergy production on European nature conservation options

    Science.gov (United States)

    Schleupner, C.; Schneider, U. A.

    2009-04-01

    To increase security of energy supply and reduce greenhouse gas (GHG) emissions the European Commission set out a long-term strategy for renewable energy in the European Union (EU). Bioenergy from forestry and agriculture plays a key role for both. Since the last decade a significant increase of biomass energy plantations has been observed in Europe. Concurrently, the EU agreed to halt the loss of biodiversity within its member states. One measure is the Natura2000 network of important nature sites that actually covers about 20% of the EU land surface. However, to fulfil the biodiversity target more nature conservation and restoration sites need to be designated. There are arising concerns that an increased cultivation of bioenergy crops will decrease the land available for nature reserves and for "traditional" agriculture and forestry. In the following the economic and ecological impacts of structural land use changes are demonstrated by two examples. First, a case study of land use changes on the Eiderstedt peninsula in Schleswig-Holstein/Germany evaluates the impacts of grassland conversion into bioenergy plantations under consideration of selected meadow birds. Scenarios indicate not only a quantitative loss of habitats but also a reduction of habitat quality. The second study assesses the role of bioenergy production in light of possible negative impacts on potential wetland conservation sites in Europe. By coupling the spatial wetland distribution model "SWEDI" (cf. SCHLEUPNER 2007) to the European Forest and Agricultural Sector Optimization Model (EUFASOM; cf. SCHNEIDER ET AL. 2008) economic and environmental aspects of land use are evaluated simultaneously. This way the costs and benefits of the appropriate measures and its consequences for agriculture and forestry are investigated. One aim is to find the socially optimal balance between alternative wetland uses by integrating biological benefits - in this case wetlands - and economic opportunities - here

  8. A systematic review of natural health product treatment for vitiligo

    Directory of Open Access Journals (Sweden)

    Boon Heather S

    2008-05-01

    Full Text Available Abstract Background Vitiligo is a hypopigmentation disorder affecting 1 to 4% of the world population. Fifty percent of cases appear before the age of 20 years old, and the disfigurement results in psychiatric morbidity in 16 to 35% of those affected. Methods Our objective was to complete a comprehensive, systematic review of the published scientific literature to identify natural health products (NHP such as vitamins, herbs and other supplements that may have efficacy in the treatment of vitiligo. We searched eight databases including MEDLINE and EMBASE for vitiligo, leucoderma, and various NHP terms. Prospective controlled clinical human trials were identified and assessed for quality. Results Fifteen clinical trials were identified, and organized into four categories based on the NHP used for treatment. 1 L-phenylalanine monotherapy was assessed in one trial, and as an adjuvant to phototherapy in three trials. All reported beneficial effects. 2 Three clinical trials utilized different traditional Chinese medicine products. Although each traditional Chinese medicine trial reported benefit in the active groups, the quality of the trials was poor. 3 Six trials investigated the use of plants in the treatment of vitiligo, four using plants as photosensitizing agents. The studies provide weak evidence that photosensitizing plants can be effective in conjunction with phototherapy, and moderate evidence that Ginkgo biloba monotherapy can be useful for vitiligo. 4 Two clinical trials investigated the use of vitamins in the therapy of vitiligo. One tested oral cobalamin with folic acid, and found no significant improvement over control. Another trial combined vitamin E with phototherapy and reported significantly better repigmentation over phototherapy only. It was not possible to pool the data from any studies for meta-analytic purposes due to the wide difference in outcome measures and poor quality ofreporting. Conclusion Reports investigating the

  9. A metabolomics guided exploration of marine natural product chemical space.

    Science.gov (United States)

    Floros, Dimitrios J; Jensen, Paul R; Dorrestein, Pieter C; Koyama, Nobuhiro

    2016-09-01

    Natural products from culture collections have enormous impact in advancing discovery programs for metabolites of biotechnological importance. These discovery efforts rely on the metabolomic characterization of strain collections. Many emerging approaches compare metabolomic profiles of such collections, but few enable the analysis and prioritization of thousands of samples from diverse organisms while delivering chemistry specific read outs. In this work we utilize untargeted LC-MS/MS based metabolomics together with molecular networking to. This approach annotated 76 molecular families (a spectral match rate of 28 %), including clinically and biotechnologically important molecules such as valinomycin, actinomycin D, and desferrioxamine E. Targeting a molecular family produced primarily by one microorganism led to the isolation and structure elucidation of two new molecules designated maridric acids A and B. Molecular networking guided exploration of large culture collections allows for rapid dereplication of know molecules and can highlight producers of uniques metabolites. These methods, together with large culture collections and growing databases, allow for data driven strain prioritization with a focus on novel chemistries.

  10. Plants’ Natural Products as Alternative Promising Anti-Candida Drugs

    Science.gov (United States)

    Soliman, Sameh; Alnajdy, Dina; El-Keblawy, Ali A.; Mosa, Kareem A.; Khoder, Ghalia; Noreddin, Ayman M.

    2017-01-01

    Candida is a serious life-threatening pathogen, particularly with immunocompromised patients. Candida infections are considered as a major cause of morbidity and mortality in a broad range of immunocompromised patients. Candida infections are common in hospitalized patients and elderly people. The difficulty to eradicate Candida infections is owing to its unique switch between yeast and hyphae forms and more likely to biofilm formations that render resistance to antifungal therapy. Plants are known sources of natural medicines. Several plants show significant anti-Candida activities and some of them have lower minimum inhibitory concentration, making them promising candidates for anti-Candida therapy. However, none of these plant products is marketed for anti-Candida therapy because of lack of sufficient information about their efficacy, toxicity, and kinetics. This review revises major plants that have been tested for anti-Candida activities with recommendations for further use of some of these plants for more investigation and in vivo testing including the use of nanostructure lipid system. PMID:28989245

  11. Natural Products for the Treatment of Chlamydiaceae Infections

    Directory of Open Access Journals (Sweden)

    Mika A. Brown

    2016-10-01

    Full Text Available Due to the global prevalence of Chlamydiae, exploring studies of diverse antichlamydial compounds is important in the development of effective treatment strategies and global infectious disease management. Chlamydiaceae is the most widely known bacterial family of the Chlamydiae order. Among the species in the family Chlamydiaceae, Chlamydia trachomatis and Chlamydia pneumoniae cause common human diseases, while Chlamydia abortus, Chlamydia psittaci, and Chlamydia suis represent zoonotic threats or are endemic in human food sources. Although chlamydial infections are currently manageable in human populations, chlamydial infections in livestock are endemic and there is significant difficulty achieving effective treatment. To combat the spread of Chlamydiaceae in humans and other hosts, improved methods for treatment and prevention of infection are needed. There exist various studies exploring the potential of natural products for developing new antichlamydial treatment modalities. Polyphenolic compounds can inhibit chlamydial growth by membrane disruption, reestablishment of host cell apoptosis, or improving host immune system detection. Fatty acids, monoglycerides, and lipids can disrupt the cell membranes of infective chlamydial elementary bodies (EBs. Peptides can disrupt the cell membranes of chlamydial EBs, and transferrins can inhibit chlamydial EBs from attachment to and permeation through the membranes of host cells. Cellular metabolites and probiotic bacteria can inhibit chlamydial infection by modulating host immune responses and directly inhibiting chlamydial growth. Finally, early stage clinical trials indicate that polyherbal formulations can be effective in treating chlamydial infections. Herein, we review an important body of literature in the field of antichlamydial research.

  12. Pop-like halogenated natural products in antarctic sponges

    Energy Technology Data Exchange (ETDEWEB)

    Vetter, W. [Hohenheim Univ., Stuttgart (Germany); Janussen, D. [Senckenbergische Naturforschende Gesellschaft (Natur-Museum und Forschungs-Institut), Frankfurt am Main (Germany)

    2004-09-15

    Persistent organic pollutants (POPs) are major contaminants of our days. This group of chemicals comprises a number of halogenated compounds used as pesticides (DDT, lindane, chlordane, toxaphene and others) as well as industrial chemicals (PCBs, PCNs, CPs, and brominated flameretardants). Although the list of known POPs including isomers and metabolites is long, there are frequent reports on the detection of unknown organohalogen compounds in the literature. Recent work demonstrated that some of these unknown peaks in gas chromatograms originate from halogenated natural products (HNPs). Sometimes, HNPs have been found at remarkably high concentrations in marine birds, mammals and fish. Due to the structural similarities with anthropogenic POPs, these substances may possess a potential risk for wildlife and man. HNPs are known to be produced with an overwhelming variety by marine organisms such as algae, sponges, microorganisms and others. In this study we have screened different species of Antarctic sponges on the occurrence of halogenated compounds which may be of environmental concern. Thus, we were only interested in lipophilic and persistent HNPs. Following that, we applied our standard sample clean-up procedure for the analysis of nonpolar POPs. Two steps on deactivated and activated silica yielded compounds with similar polarity as PCBs, chloropesticides and brominated analogues in the sample extracts. Additionally, all samples were treated with concentrated sulphuric acid in order to eliminate labile (non-presistent) HNPs.

  13. Selective Killing of Dormant Mycobacterium tuberculosis by Marine Natural Products.

    Science.gov (United States)

    Rodrigues Felix, Carolina; Gupta, Rashmi; Geden, Sandra; Roberts, Jill; Winder, Priscilla; Pomponi, Shirley A; Diaz, Maria Cristina; Reed, John K; Wright, Amy E; Rohde, Kyle H

    2017-08-01

    The dormant phenotype acquired by Mycobacterium tuberculosis during infection poses a major challenge in disease treatment, since these bacilli show tolerance to front-line drugs. Therefore, it is imperative to find novel compounds that effectively kill dormant bacteria. By screening 4,400 marine natural product samples against dual-fluorescent M. tuberculosis under both replicating and nonreplicating conditions, we have identified compounds that are selectively active against dormant M. tuberculosis This validates our strategy of screening all compounds in both assays as opposed to using the dormancy model as a secondary screen. Bioassay-guided deconvolution enabled the identification of unique pharmacophores active in each screening model. To confirm the activity of samples against dormant M. tuberculosis , we used a luciferase reporter assay and enumerated CFU. The structures of five purified active compounds were defined by nuclear magnetic resonance (NMR) and mass spectrometry. We identified two lipid compounds with potent activity toward dormant and actively growing M. tuberculosis strains. One of these was commercially obtained and showed similar activity against M. tuberculosis in both screening models. Furthermore, puupehenone-like molecules were purified with potent and selective activity against dormant M. tuberculosis In conclusion, we have identified and characterized antimycobacterial compounds from marine organisms with novel activity profiles which appear to target M. tuberculosis pathways that are conditionally essential for dormancy survival. Copyright © 2017 American Society for Microbiology.

  14. Identification and quantification of the halogenated natural product BC-3

    Energy Technology Data Exchange (ETDEWEB)

    Melcher, J.; Olbrich, D.; Vetter, W. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Lebensmittelchemie; Marsh, G. [Stockholm Univ. (Sweden). Dept. of Environmental Chemistry; Gaus, C.; Mueller, J.F. [National Research Centre for Environmental Toxicology, Coopers Plains (Australia)

    2004-09-15

    Halogenated natural products (HNPs) of marine origin are increasingly recognized as critical residues in foodstuff (e. g. fish) and environmental samples (e. g. marine mammals and birds). Some of these HNPs (Q1, MHC-1, BC-2, and HDBPs including BC-10) were detected in diverse fish and marine mammal samples at concentrations sometimes exceeding those of PCBs, DDT, and other anthropogenic pollutants. Recent studies with marine mammal samples from Australia led to the detection of six abundant HNPs (Q1, BC-1, BC-2, BC-3, BC-10, and BC-11). In the meantime, Q1 was identified as heptachloro-1{sup '}-methyl-1,2{sup '}-bipyrrole, BC-2 as 4,6-dibromo-2-(2{sup '},4{sup '}-dibromo)phenoxyanisole, BC- 10 as 1,1{sup '}-dimethyl-3,3{sup '},4,4{sup '}-tetrabromo-5,5{sup '}-dichloro-2,2{sup '}-bipyrrole, and BC-11 as 3,5-dibromo- 2-(3{sup '},5{sup '}-dibromo,2{sup '}-methoxy)phenoxyanisole. However the identity of BC-1 and BC-3 remained unclear. The goal of the present study was the identification of BC-3. The tetrabromo compound BC-3 has previously been detected in marine mammals from four continents. Furthermore, we attempted establishing quantitative concentrations in diverse marine biota samples.

  15. Natural Products as Leads in Schistosome Drug Discovery

    Directory of Open Access Journals (Sweden)

    Bruno J. Neves

    2015-01-01

    Full Text Available Schistosomiasis is a neglected parasitic tropical disease that claims around 200,000 human lives every year. Praziquantel (PZQ, the only drug recommended by the World Health Organization for the treatment and control of human schistosomiasis, is now facing the threat of drug resistance, indicating the urgent need for new effective compounds to treat this disease. Therefore, globally, there is renewed interest in natural products (NPs as a starting point for drug discovery and development for schistosomiasis. Recent advances in genomics, proteomics, bioinformatics, and cheminformatics have brought about unprecedented opportunities for the rapid and more cost-effective discovery of new bioactive compounds against neglected tropical diseases. This review highlights the main contributions that NP drug discovery and development have made in the treatment of schistosomiasis and it discusses how integration with virtual screening (VS strategies may contribute to accelerating the development of new schistosomidal leads, especially through the identification of unexplored, biologically active chemical scaffolds and structural optimization of NPs with previously established activity.

  16. Formulating natural based cosmetic product - irradiated herbal lip balm

    International Nuclear Information System (INIS)

    Seri Chempaka Mohd Yusof; Ros Anita Ahmad Ramli; Foziah Ali; Zainab Harun

    2007-01-01

    Herbal lip balm was formulated in efforts to produce a safe product, attractive with multifunctional usage i.e. prevent chap lips, reduce mouth odour and benefits in improving the health quality. Problems faced in constructing formulations of herbal lip balm were focused to the extraction of anthocyanins, the stability of the pigments in the formulations and changes of colour during irradiation for the sterilization of herbal lip balm. Natural pigment, anthocyanin was used as a colorant agent in herbal lip balm, obtained from various herbs and vegetables i.e. Hibiscus sabdariffa L. (roselle), Brassica oleracea var. capitata f. rubra (red cabbage) and Daucus carota (carrot). Water based extraction method was used in extracting the anthocyanins. The incorporation of honey in the formulations improved the colour of the lip balm. The usage of plant based ingredient i.e. cocoa butter substituting the normal based ingredient i.e. petroleum jelly in lip balm also affecting the colour of herbal lip balm. Irradiation at 2.5, 5.0 and 10 kGy was carried out as preservation and reducing of microbial load of the herbal lip balm and changes in colour were observed in formulations irradiated at 10 kGy. (Author)

  17. Natural Product Screening Reveals Naphthoquinone Complex I Bypass Factors.

    Directory of Open Access Journals (Sweden)

    Scott B Vafai

    Full Text Available Deficiency of mitochondrial complex I is encountered in both rare and common diseases, but we have limited therapeutic options to treat this lesion to the oxidative phosphorylation system (OXPHOS. Idebenone and menadione are redox-active molecules capable of rescuing OXPHOS activity by engaging complex I-independent pathways of entry, often referred to as "complex I bypass." In the present study, we created a cellular model of complex I deficiency by using CRISPR genome editing to knock out Ndufa9 in mouse myoblasts, and utilized this cell line to develop a high-throughput screening platform for novel complex I bypass factors. We screened a library of ~40,000 natural product extracts and performed bioassay-guided fractionation on a subset of the top scoring hits. We isolated four plant-derived 1,4-naphthoquinone complex I bypass factors with structural similarity to menadione: chimaphilin and 3-chloro-chimaphilin from Chimaphila umbellata and dehydro-α-lapachone and dehydroiso-α-lapachone from Stereospermum euphoroides. We also tested a small number of structurally related naphthoquinones from commercial sources and identified two additional compounds with complex I bypass activity: 2-methoxy-1,4-naphthoquinone and 2-methoxy-3-methyl-1,4,-naphthoquinone. The six novel complex I bypass factors reported here expand this class of molecules and will be useful as tool compounds for investigating complex I disease biology.

  18. QSAR Study of the Biologically Active Organosulfurs in Natural Products

    Directory of Open Access Journals (Sweden)

    Fakhr M. Abu-Awwad

    2010-01-01

    Full Text Available The biological activities of two sets of a total of 30 different polysulfides were investigated using QSAR. The semiempirical AM1 in Gaussian 2003 for windows was used to optimize the structures whereas a subsequent calculation of hundreds of various types of descriptors at the density functional (B3LYP/6-31G* using CODESSA package was employed. The known 15-lipoxygenase inhibitory activity data (IC50 of 19 polysulfides out of the whole data set were correlated in a multiple linear regression procedures with the computed descriptors. Statistically, the most significant overall correlations were five- and four- parameter equations with good statistical parameters; R2= 0.9981, R2CV = 0.9970 and R2=9967, R2CV = 0.9933 respectively. The models concluded that biological activity of polysulfides is mainly attributed to quantum-chemical, geometrical and topological descriptors with neither electrostatic contribution nor chief role of sulfur atoms. Also sulfur related descriptors were not the most significant contributors in the concluded models. The obtained models were efficiently employed to estimate the biological activities of the other 11 polysulfides available in natural products such as garlic and mushroom.

  19. An analysis of FDA-approved drugs: natural products and their derivatives.

    Science.gov (United States)

    Patridge, Eric; Gareiss, Peter; Kinch, Michael S; Hoyer, Denton

    2016-02-01

    Natural products contribute greatly to the history and landscape of new molecular entities (NMEs). An assessment of all FDA-approved NMEs reveals that natural products and their derivatives represent over one-third of all NMEs. Nearly one-half of these are derived from mammals, one-quarter from microbes and one-quarter from plants. Since the 1930s, the total fraction of natural products has diminished, whereas semisynthetic and synthetic natural product derivatives have increased. Over time, this fraction has also become enriched with microbial natural products, which represent a significant portion of approved antibiotics, including more than two-thirds of all antibacterial NMEs. In recent years, the declining focus on natural products has impacted the pipeline of NMEs from specific classes, and this trend is likely to continue without specific investment in the pursuit of natural products. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Myxobacterial natural products: An under-valued source of products for drug discovery for neurological disorders.

    Science.gov (United States)

    Dehhaghi, Mona; Mohammadipanah, Fatemeh; Guillemin, Gilles J

    2018-03-02

    Age-related disorders impose noticeable financial and emotional burdens on society. This impact is becoming more prevalent with the increasing incidence of neurodegenerative diseases and is causing critical concerns for treatment of patients worldwide. Parkinson's disease, Alzheimer's disease, multiple sclerosis and motor neuron disease are the most prevalent and the most expensive to treat neurodegenerative diseases globally. Therefore, exploring effective therapies to overcome these disorders is a necessity. Natural products and their derivatives have increasingly attracted attention in drug discovery programs that have identified microorganisms which produce a large range of metabolites with bioactive properties. Myxobacteria, a group of Gram-negative bacteria with large genome size, produce a wide range of secondary metabolites with significant chemical structures and a variety of biological effects. They are potent natural product producers. In this review paper, we attempt to overview some secondary metabolites synthesized by myxobacteria with neuroprotective activity through known mechanisms including production of polyunsaturated fatty acids, reduction of apoptosis, immunomodulation, stress reduction of endoplasmic reticulum, stabilization of microtubules, enzyme inhibition and serotonin receptor modulation. Copyright © 2018 Elsevier B.V. All rights reserved.

  1. Ethanol production by recombinant and natural xylose-utilising yeasts

    Energy Technology Data Exchange (ETDEWEB)

    Eliasson, Anna

    2000-07-01

    The xylose-fermenting capacity of recombinant Saccharomyces cerevisiae carrying XYL1 and XYL2 from Pichia stipitis, which encode xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, is poor due to high xylitol formation. Whereas, P. stipitis exhibits high ethanol yield on xylose, the tolerance towards inhibitors in the lignocellulosic hydrolysate is low. A recombinant strain possessing the advantageous characteristics of both S. cerevisiae and P. stipitis would constitute a biocatalyst capable of efficient ethanol production from lignocellulosic hydrolysate. In the work presented in this thesis, factors influencing xylose fermentation in recombinant S. cerevisiae and in the natural xylose-fermenting yeast P. stipitis have been identified and investigated. Anaerobic xylulose fermentation was compared in strains of Zygosaccharomyces and S. cerevisiae, mutants and wild-type strains to identify host strain background and genetic modifications beneficial for xylose fermentation. The greatest positive effect was found for over-expression of the gene XKS1 for the pentose phosphate pathway (PPP) enzyme xylulokinase (XK), which increased the ethanol yield by almost 85%. The Zygosaccharomyces strains tested formed large amounts of polyols, making them unsuitable as host strains. The XR/XDH/XK ratio was found to determine whether carbon accumulated in a xylitol pool or was further utilised for ethanol production in recombinant xylose-utilising S. cerevisiae. Simulations, based on a kinetic model, and anaerobic xylose cultivation experiments implied that a 1:{>=}10:{>=}4 relation was optimal in minimising xylitol formation. Ethanol formation increased with decreasing XR/XDH ratio, whereas xylitol formation decreased and XK overexpression was necessary for adequate ethanol formation. Based on the knowledge of optimal enzyme ratios, a stable, xylose-utilising strain, S. cerevisiae TMB 3001, was constructed by chromosomal integration of the XYL1 and XYL2 genes

  2. Data Resources for the Computer-Guided Discovery of Bioactive Natural Products.

    Science.gov (United States)

    Chen, Ya; de Bruyn Kops, Christina; Kirchmair, Johannes

    2017-09-25

    Natural products from plants, animals, marine life, fungi, bacteria, and other organisms are an important resource for modern drug discovery. Their biological relevance and structural diversity make natural products good starting points for drug design. Natural product-based drug discovery can benefit greatly from computational approaches, which are a valuable precursor or supplementary method to in vitro testing. We present an overview of 25 virtual and 31 physical natural product libraries that are useful for applications in cheminformatics, in particular virtual screening. The overview includes detailed information about each library, the extent of its structural information, and the overlap between different sources of natural products. In terms of chemical structures, there is a large overlap between freely available and commercial virtual natural product libraries. Of particular interest for drug discovery is that at least ten percent of known natural products are readily purchasable and many more natural products and derivatives are available through on-demand sourcing, extraction and synthesis services. Many of the readily purchasable natural products are of small size and hence of relevance to fragment-based drug discovery. There are also an increasing number of macrocyclic natural products and derivatives becoming available for screening.

  3. Deposits of naturally occurring radioactivity in production of oil and natural gas; Radioaktive avleiringer i olje- og gassproduksjon

    Energy Technology Data Exchange (ETDEWEB)

    Strand, T.; Lysebo, I.; Kristensen, D.; Birovljev, A.

    1997-01-01

    Deposits of naturally occurring radioactive materials is an increasing problem in Norwegian oil and gas production. Activity concentration in solid-state samples and production water, and doses to workers involved in different operations off-shore, have been measured. The report also includes a discussion of different methods of monitoring and alternatives for final disposal of wastes. 154 refs.

  4. On the nature of the new product strategy

    DEFF Research Database (Denmark)

    Larsson, Flemming

    2006-01-01

    The aim of this paper is to explore the concept of a new product strategy and its relation to portfolio management for product development. Based on a literature review this research adheres to one perception of the new product strategy proposed by literature complemented with a component adressing...... the risk-dimension. Next, it is suggested that the new product strategy is embedded in the product development strategy. Subsequently, it is advocated that companies try to achieve a base for realizing integration, synchronization and strategic alignment by means of a new product strategy. These three...... themes are explicated in a model, which also indicates the relation between the new product strategy and the product development process. Finally, implications for management practice are devised....

  5. The chemistry and biology of guanidine natural products.

    Science.gov (United States)

    Berlinck, Roberto G S; Bertonha, Ariane F; Takaki, Mirelle; Rodriguez, Julie P G

    2017-11-15

    Covering: 2015 and 2016The chemistry and biology of natural guanidines isolated from microbial culture media, from marine invertebrates, as well as from terrestrial plants and animals, are reviewed. Emphasis is directed to the biosynthesis, total synthesis, ecological roles as well as on the evolution of guanidines isolated from natural sources.

  6. Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production.

    Science.gov (United States)

    Liu, Xiaonan; Ding, Wentao; Jiang, Huifeng

    2017-07-19

    Plant natural products (PNPs) are widely used as pharmaceuticals, nutraceuticals, seasonings, pigments, etc., with a huge commercial value on the global market. However, most of these PNPs are still being extracted from plants. A resource-conserving and environment-friendly synthesis route for PNPs that utilizes microbial cell factories has attracted increasing attention since the 1940s. However, at the present only a handful of PNPs are being produced by microbial cell factories at an industrial scale, and there are still many challenges in their large-scale application. One of the challenges is that most biosynthetic pathways of PNPs are still unknown, which largely limits the number of candidate PNPs for heterologous microbial production. Another challenge is that the metabolic fluxes toward the target products in microbial hosts are often hindered by poor precursor supply, low catalytic activity of enzymes and obstructed product transport. Consequently, despite intensive studies on the metabolic engineering of microbial hosts, the fermentation costs of most heterologously produced PNPs are still too high for industrial-scale production. In this paper, we review several aspects of PNP production in microbial cell factories, including important design principles and recent progress in pathway mining and metabolic engineering. In addition, implemented cases of industrial-scale production of PNPs in microbial cell factories are also highlighted.

  7. Using Natural Products to Treat Resistant and Persistent Bacterial Infections

    Science.gov (United States)

    Deering, Robert W.

    Antimicrobial resistance is a growing threat to human health both worldwide and in the United States. Most concerning is the emergence of multi-drug resistant (MDR) bacterial pathogens, especially the 'ESKAPE' pathogens for which treatment options are dwindling. To complicate the problem, approvals of antibiotic drugs are extremely low and many research and development efforts in the pharmaceutical industry have ceased, leaving little certainty that critical new antibiotics are nearing the clinic. New antibiotics are needed to continue treating these evolving infections. In addition to antibiotics, approaches that aim to inhibit or prevent antimicrobial resistance could be useful. Also, studies that improve our understanding of bacterial pathophysiology could lead to new therapies for infectious disease. Natural products, especially those from the microbial world, have been invaluable as resources for new antibacterial compounds and as insights into bacterial physiology. The goal of this dissertation is to find new ways to treat resistant bacterial infections and learn more about the pathophysiology of these bacteria. Investigations of natural products to find molecules able to be used as new antibiotics or to modulate resistance and other parts of bacterial physiology are crucial aspects of the included studies. The first included study, which is reported in chapter two, details a chemical investigation of a marine Pseudoalteromonas sp. Purification efforts of the microbial metabolites were guided by testing against a resistance nodulation of cell division model of efflux pumps expressed in E. coli. These pumps play an important role in the resistance of MDR Gram negative pathogens such as Pseudomonas aeruginosa and Enterobacteriaceae. Through this process, 3,4-dibromopyrrole-2,5-dione was identified as a potent inhibitor of the RND efflux pumps and showed synergistic effects against the E. coli strain with common antibiotics including fluoroquinolones, beta

  8. On the nature of the new product strategy

    DEFF Research Database (Denmark)

    Larsson, Flemming

    2006-01-01

    the risk-dimension. Next, it is suggested that the new product strategy is embedded in the product development strategy. Subsequently, it is advocated that companies try to achieve a base for realizing integration, synchronization and strategic alignment by means of a new product strategy. These three...

  9. Comparative analysis of chemical similarity methods for modular natural products with a hypothetical structure enumeration algorithm.

    Science.gov (United States)

    Skinnider, Michael A; Dejong, Chris A; Franczak, Brian C; McNicholas, Paul D; Magarvey, Nathan A

    2017-08-16

    Natural products represent a prominent source of pharmaceutically and industrially important agents. Calculating the chemical similarity of two molecules is a central task in cheminformatics, with applications at multiple stages of the drug discovery pipeline. Quantifying the similarity of natural products is a particularly important problem, as the biological activities of these molecules have been extensively optimized by natural selection. The large and structurally complex scaffolds of natural products distinguish their physical and chemical properties from those of synthetic compounds. However, no analysis of the performance of existing methods for molecular similarity calculation specific to natural products has been reported to date. Here, we present LEMONS, an algorithm for the enumeration of hypothetical modular natural product structures. We leverage this algorithm to conduct a comparative analysis of molecular similarity methods within the unique chemical space occupied by modular natural products using controlled synthetic data, and comprehensively investigate the impact of diverse biosynthetic parameters on similarity search. We additionally investigate a recently described algorithm for natural product retrobiosynthesis and alignment, and find that when rule-based retrobiosynthesis can be applied, this approach outperforms conventional two-dimensional fingerprints, suggesting it may represent a valuable approach for the targeted exploration of natural product chemical space and microbial genome mining. Our open-source algorithm is an extensible method of enumerating hypothetical natural product structures with diverse potential applications in bioinformatics.

  10. Natural personal care products-analysis of ingredient lists and legal situation.

    Science.gov (United States)

    Klaschka, Ursula

    2016-01-01

    Many natural substances are classified as dangerous substances according to the European regulation on classification and labelling. Are they used in natural personal care products today? One hundred ingredient lists were analyzed to find this out. All products with natural substances contained dangerous natural substances or they contained natural substances, for which the information about their classification as dangerous substances is not available. 54 natural substances quoted in the ingredient lists were found to be classified, with 37 substances being classified due to hazardous effects for skin and eyes. However, the most frequently used natural substances are not classified as dangerous. Natural substances are multi-constituent compounds, leading to two main problems in personal care products: the potential interactions of a multitude of substances and the fact that dangerous constituents are not disclosed in the ingredient lists. For example, the fragrance allergens citral, farnesol, limonene, and linalool are frequent components of the natural substances employed. In addition, 82 products listed allergenic fragrance ingredients as single substances in their ingredient lists. Recommendations for sensitive skin in a product's name do not imply that the '26 fragrance allergens' are omitted. Furthermore, 80 products listed 'parfum'/'aroma', and 50 products listed ethanol. The data show that the loopholes for natural substances and for personal care products in the present European chemical legislation (e.g. the exception for classification and labelling of cosmetic products and the exception for information transfer in the supply chain) are not in line with an adequate consumer and environmental protection.

  11. Petroleum and natural gas: exploration and production in France during the last ten years

    International Nuclear Information System (INIS)

    Combaz, A.

    1993-01-01

    This paper presents petroleum and natural gas exploration in France, during the last ten years. Statistical data on production, petroleum and natural gas deposits and forecasting are also given. 4 refs., 11 figs

  12. New natural antioxidants for protecting omega-3 rich products

    DEFF Research Database (Denmark)

    Farvin, Sabeena; Jacobsen, Charlotte

    2012-01-01

    antioxidants from yoghurt, potato peel, fish protein hydrolysates and seaweed, which were able to protect highly unsaturated fish oil from oxidation. These antioxidant extracts may have potential for commercial exploitation. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim....... to oxidation because of their unsaturated nature. Addition of antioxidants is one method. to prevent lipid oxidation. As synthetic antioxidants may have carcinogenic effects at higher levels, the replacement of synthetic antioxidants with natural antioxidants is now in demand. We have isolated natural...

  13. Cheminformatic comparison of approved drugs from natural product versus synthetic origins.

    Science.gov (United States)

    Stratton, Christopher F; Newman, David J; Tan, Derek S

    2015-11-01

    Despite the recent decline of natural product discovery programs in the pharmaceutical industry, approximately half of all new drug approvals still trace their structural origins to a natural product. Herein, we use principal component analysis to compare the structural and physicochemical features of drugs from natural product-based versus completely synthetic origins that were approved between 1981 and 2010. Drugs based on natural product structures display greater chemical diversity and occupy larger regions of chemical space than drugs from completely synthetic origins. Notably, synthetic drugs based on natural product pharmacophores also exhibit lower hydrophobicity and greater stereochemical content than drugs from completely synthetic origins. These results illustrate that structural features found in natural products can be successfully incorporated into synthetic drugs, thereby increasing the chemical diversity available for small-molecule drug discovery. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Textbook for nature entrepreneurship : product of the WURKS project Nature Entrepreneurship and Tourism within Green Education (NatureToGo)

    NARCIS (Netherlands)

    Felder, M.; Pellis, A.

    2013-01-01

    In recent years, government funding for nature conservation and development has declined. As a result, links between nature conservation and entrepreneurship are increasingly being made in both practice and education. This comes with many questions and challenges. In Green Secondary Vocational

  15. Short history of natural product research in the CSIR

    CSIR Research Space (South Africa)

    Walwyn, D

    2006-02-01

    Full Text Available to develop the country's natural resource. This poster is a short summary of the research, including the identification of drug leads, characterisation of food spoilage organisms and toxin, especially mycotoxins, and rumen digestion. It describes some...

  16. Metagenomic Approaches to Natural Products from Free-Living and Symbiotic Organisms

    OpenAIRE

    Brady, Sean F.; Simmons, Luke; Kim, Jeff H.; Schmidt, Eric W.

    2009-01-01

    Bacterial cultivation has been a mainstay of natural products discovery for the past 80 years. However, the majority of bacteria are recalcitrant to culture, providing an untapped source for new natural products. Metagenomic analysis provides an alternative method to directly access the uncultivated genome for natural products research and for the discovery of novel, bioactive substances. Applications of metagenomics to diverse habitats, such as soils and the interior of animals, are described.

  17. Natural products as tools for studies of ligand-gated ion channels

    DEFF Research Database (Denmark)

    Strømgaard, Kristian

    2005-01-01

    in the brain. Historically, natural products have been used extensively in biomedical studies and ultimately as drugs or leads for drug design. In studies of ligand-gated ion channels, natural products have been essential for the understanding of their structure and function. In the following a short survey...... of natural products and their use in studies of ligand-gated ion channels is given....

  18. Natural Language Assistant: A Dialog System for Online Product Recommendation

    OpenAIRE

    Chai, Joyce; Horvath, Veronika; Nicolov, Nicolas; Stys, Margo; Kambhatla, Nanda; Zadrozny, Wlodek; Melville, Prem

    2002-01-01

    With the emergence of electronic-commerce systems, successful information access on electroniccommerce web sites becomes essential. Menu-driven navigation and keyword search currently provided by most commercial sites have considerable limitations because they tend to overwhelm and frustrate users with lengthy, rigid, and ineffective interactions. To provide an efficient solution for information access, we have built the NATURAL language ASSISTANT (NLA), a web-based natural language dialog sy...

  19. Pharmacognosy: Science of natural products in drug discovery.

    Science.gov (United States)

    Orhan, Ilkay Erdogan

    2014-01-01

    Pharmacognosy deals with the natural drugs obtained from organisms such as most plants, microbes, and animals. Up to date, many important drugs including morphine, atropine, galanthamine, etc. have originated from natural sources which continue to be good model molecules in drug discovery. Traditional medicine is also a part of pharmacognosy and most of the third world countries still depend on the use of herbal medicines. Consequently, pharmacognosy always keeps its popularity in pharmaceutical sciences and plays a critical role in drug discovery.

  20. Retrospective analysis of natural products provides insights for future discovery trends

    Science.gov (United States)

    Pye, Cameron R.; Bertin, Matthew J.; Lokey, R. Scott; Gerwick, William H.

    2017-01-01

    Understanding of the capacity of the natural world to produce secondary metabolites is important to a broad range of fields, including drug discovery, ecology, biosynthesis, and chemical biology, among others. Both the absolute number and the rate of discovery of natural products have increased significantly in recent years. However, there is a perception and concern that the fundamental novelty of these discoveries is decreasing relative to previously known natural products. This study presents a quantitative examination of the field from the perspective of both number of compounds and compound novelty using a dataset of all published microbial and marine-derived natural products. This analysis aimed to explore a number of key questions, such as how the rate of discovery of new natural products has changed over the past decades, how the average natural product structural novelty has changed as a function of time, whether exploring novel taxonomic space affords an advantage in terms of novel compound discovery, and whether it is possible to estimate how close we are to having described all of the chemical space covered by natural products. Our analyses demonstrate that most natural products being published today bear structural similarity to previously published compounds, and that the range of scaffolds readily accessible from nature is limited. However, the analysis also shows that the field continues to discover appreciable numbers of natural products with no structural precedent. Together, these results suggest that the development of innovative discovery methods will continue to yield compounds with unique structural and biological properties. PMID:28461474

  1. Informatic search strategies to discover analogues and variants of natural product archetypes.

    Science.gov (United States)

    Johnston, Chad W; Connaty, Alex D; Skinnider, Michael A; Li, Yong; Grunwald, Alyssa; Wyatt, Morgan A; Kerr, Russell G; Magarvey, Nathan A

    2016-03-01

    Natural products are a crucial source of antimicrobial agents, but reliance on low-resolution bioactivity-guided approaches has led to diminishing interest in discovery programmes. Here, we demonstrate that two in-house automated informatic platforms can be used to target classes of biologically active natural products, specifically, peptaibols. We demonstrate that mass spectrometry-based informatic approaches can be used to detect natural products with high sensitivity, identifying desired agents present in complex microbial extracts. Using our specialised software packages, we could elaborate specific branches of chemical space, uncovering new variants of trichopolyn and demonstrating a way forward in mining natural products as a valuable source of potential pharmaceutical agents.

  2. Environmental solutions for the sustainable production of bioactive natural products from the marine sponge Crambe crambe.

    Science.gov (United States)

    Pérez-López, Paula; Ternon, Eva; González-García, Sara; Genta-Jouve, Grégory; Feijoo, Gumersindo; Thomas, Olivier P; Moreira, Ma Teresa

    2014-03-15

    Crambe crambe is a Mediterranean marine sponge known to produce original natural substances belonging to two families of guanidine alkaloids, namely crambescins and crambescidins, which exhibit cytotoxic and antiviral activities. These compounds are therefore considered as potential anticancer drugs. The present study focuses on the environmental assessment of a novel in vivo process for the production of pure crambescin and crambescidin using sponge specimens cultured in aquarium. The assessment was performed following the ISO 14040 standard and extended from the production of the different mass and energy flows to the system to the growth of the sponge in indoor aquarium and further periodic extraction and purification of the bioactive compounds. According to the results, the two stages that have a remarkable contribution to all impact categories are the purification of the bioactive molecules followed by the maintenance of the sponge culture in the aquarium. Among the involved activities, the production of the chemicals (particularly methanol) together with the electricity requirements (especially due to the aquarium lighting) are responsible for up to 90% of the impact in most of the assessed categories. However, the contributions of other stages to the environmental burdens, such as the collection of sponges, considerably depend on the assumptions made during the inventory stage. The simulation of alternative scenarios has led to propose improvement alternatives that may allow significant reductions ranging from 20% to 70%, mainly thanks to the reduction of electricity requirements as well as the partial reuse of methanol. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. High folate production by naturally occurring Lactobacillus sp. with ...

    African Journals Online (AJOL)

    Rapidly proliferating cells require large amounts of folate to support efficient DNA replication, repair and methylation indicating the importance of folate in human metabolism. Milk products are good sources of such vitamins which are produced by probiotics. In order to find suitable strains capable of high folate production, ...

  4. Lessons learned from the transformation of natural product discovery to a genome-driven endeavor

    Science.gov (United States)

    Deane, Caitlin D.; Mitchell, Douglas A.

    2013-01-01

    Natural product discovery is currently undergoing a transformation from a phenotype-driven field to a genotype-driven one. The increasing availability of genome sequences, coupled with improved techniques for identifying biosynthetic gene clusters, has revealed that secondary metabolomes are strikingly vaster than previously thought. New approaches to correlate biosynthetic gene clusters with the compounds they produce have facilitated the production and isolation of a rapidly growing collection of what we refer to as “reverse-discovered” natural products, in analogy to reverse genetics. In this review, we present an extensive list of reverse-discovered natural products and discuss seven important lessons for natural product discovery by genome-guided methods: structure prediction, accurate annotation, continued study of model organisms, avoiding genome size bias, genetic manipulation, heterologous expression, and potential engineering of natural product analogs. PMID:24142337

  5. Nature Relation Between Climatic Variables and Cotton Production

    Directory of Open Access Journals (Sweden)

    Zakaria M. Sawan

    2014-08-01

    Full Text Available This study investigated the effect of climatic variables on flower and boll production and retention in cotton (Gossypium barbadense. Also, this study investigated the relationship between climatic factors and production of flowers and bolls obtained during the development periods of the flowering and boll stage, and to determine the most representative period corresponding to the overall crop pattern. Evaporation, sunshine duration, relative humidity, surface soil temperature at 1800 h, and maximum air temperature, are the important climatic factors that significantly affect flower and boll production. The least important variables were found to be surface soil temperature at 0600 h and minimum temperature. There was a negative correlation between flower and boll production and either evaporation or sunshine duration, while that correlation with minimum relative humidity was positive. Higher minimum relative humidity, short period of sunshine duration, and low temperatures enhanced flower and boll formation.

  6. Use of natural zeolite-supplemented litter increased broiler production

    African Journals Online (AJOL)

    The aim of this study was to ascertain the influence of natural zeolite, consisting mainly of clinoptilolite and mordenite, as a component of the litter material in broiler houses on the performance of the broilers and on some litter characteristics. Live weight gain, feed consumption, feed efficiency, viability and leg and body ...

  7. Use of natural zeolite-supplemented litter increased broiler production

    African Journals Online (AJOL)

    smyo

    Abstract. The aim of this study was to ascertain the influence of natural zeolite, consisting mainly of clinoptilolite and mordenite, as a component of the litter material in broiler houses on the performance of the broilers and on some litter characteristics. Live weight gain, feed consumption, feed efficiency, viability and leg and ...

  8. Voracious transformation of a common natural resource into productive capital

    NARCIS (Netherlands)

    van der Ploeg, F.

    2010-01-01

    I analyze a power struggle where competing factions have private financial assets and deplete a common stock of natural resources with no private property rights. I obtain a feedback Nash equilibrium to the dynamic common-pool problem and obtain political variants of the Hotelling depletion rule and

  9. ​Affordable natural product reduces fruit losses, increases incomes ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Taking new technologies to market Research on scaling up will identify suitable, practical methods for wide-scale adoption and market entry. ... Breakthrough research by Canadian, Indian, and Sri Lankan partners points to a promising innovation: nanotech applications of a natural plant extract called hexanal can be used ...

  10. Structural modeling of natural citrus products as potential cross ...

    African Journals Online (AJOL)

    There are four serotypes of Dengue virus and there are existing drugs used against specific serotype. There is no drug that is effective against all strains of this virus. In this research, bioinformatics tools were used to predict the affinity of natural ligands for the glycoprotein E of Dengue virus by considering the conserved ...

  11. Nature Relation Between Climatic Variables and Cotton Production

    OpenAIRE

    Zakaria M. Sawan

    2014-01-01

    This study investigated the effect of climatic variables on flower and boll production and retention in cotton ( Gossypium barbadense ). Also, this study investigated the relationship between climatic factors and production of flowers and bolls obtained during the development periods of the flowering and boll stage, and to determine the most representative period corresponding to the overall crop pattern. Evaporation, sunshine duration, relative humidity, surface soil temperature at 1800 h, a...

  12. Natural Gas and Cellulosic Biomass: A Clean Fuel Combination? Determining the Natural Gas Blending Wall in Biofuel Production.

    Science.gov (United States)

    M Wright, Mark; Seifkar, Navid; Green, William H; Román-Leshkov, Yuriy

    2015-07-07

    Natural gas has the potential to increase the biofuel production output by combining gas- and biomass-to-liquids (GBTL) processes followed by naphtha and diesel fuel synthesis via Fischer-Tropsch (FT). This study reflects on the use of commercial-ready configurations of GBTL technologies and the environmental impact of enhancing biofuels with natural gas. The autothermal and steam-methane reforming processes for natural gas conversion and the gasification of biomass for FT fuel synthesis are modeled to estimate system well-to-wheel emissions and compare them to limits established by U.S. renewable fuel mandates. We show that natural gas can enhance FT biofuel production by reducing the need for water-gas shift (WGS) of biomass-derived syngas to achieve appropriate H2/CO ratios. Specifically, fuel yields are increased from less than 60 gallons per ton to over 100 gallons per ton with increasing natural gas input. However, GBTL facilities would need to limit natural gas use to less than 19.1% on a LHV energy basis (7.83 wt %) to avoid exceeding the emissions limits established by the Renewable Fuels Standard (RFS2) for clean, advanced biofuels. This effectively constitutes a blending limit that constrains the use of natural gas for enhancing the biomass-to-liquids (BTL) process.

  13. Prediction of cancer cell sensitivity to natural products based on genomic and chemical properties

    Directory of Open Access Journals (Sweden)

    Zhenyu Yue

    2015-11-01

    Full Text Available Natural products play a significant role in cancer chemotherapy. They are likely to provide many lead structures, which can be used as templates for the construction of novel drugs with enhanced antitumor activity. Traditional research approaches studied structure-activity relationship of natural products and obtained key structural properties, such as chemical bond or group, with the purpose of ascertaining their effect on a single cell line or a single tissue type. Here, for the first time, we develop a machine learning method to comprehensively predict natural products responses against a panel of cancer cell lines based on both the gene expression and the chemical properties of natural products. The results on two datasets, training set and independent test set, show that this proposed method yields significantly better prediction accuracy. In addition, we also demonstrate the predictive power of our proposed method by modeling the cancer cell sensitivity to two natural products, Curcumin and Resveratrol, which indicate that our method can effectively predict the response of cancer cell lines to these two natural products. Taken together, the method will facilitate the identification of natural products as cancer therapies and the development of precision medicine by linking the features of patient genomes to natural product sensitivity.

  14. Direct Capture Technologies for Genomics-Guided Discovery of Natural Products

    Science.gov (United States)

    Chan, Andrew N.; Santa Maria, Kevin C.; Li, Bo

    2016-01-01

    Microbes are important producers of natural products, which have played key roles in understanding biology and treating disease. However, the full potential of microbes to produce natural products has yet to be realized; the overwhelming majority of natural product gene clusters encoded in microbial genomes remain “cryptic”, and have not been expressed or characterized. In contrast to the fast-growing number of genomic sequences and bioinformatic tools, methods to connect these genes to natural product molecules are still limited, creating a bottleneck in genome-mining efforts to discover novel natural products. Here we review developing technologies that leverage the power of homologous recombination to directly capture natural product gene clusters and express them in model hosts for isolation and structural characterization. Although direct capture is still in its early stages of development, it has been successfully utilized in several different classes of natural products. These early successes will be reviewed, and the methods will be compared and contrasted with existing traditional technologies. Lastly, we will discuss the opportunities for the development of direct capture in other organisms, and possibilities to integrate direct capture with emerging genome-editing techniques to accelerate future study of natural products. PMID:26456469

  15. Discovery of phosphonic acid natural products by mining the genomes of 10,000 actinomycetes

    Science.gov (United States)

    Although natural products have been a particularly rich source of human medicines, the rate at which new molecules are being discovered is declining precipitously. Based on the large number of natural product biosynthetic genes in microbial genomes, many have suggested “genome mining” as an approach...

  16. Improving Agricultural Productivity with Radiation Processed Natural Polymers

    International Nuclear Information System (INIS)

    Henriques, Sasha

    2013-01-01

    Full text: Through the power of irradiation to break and create chemical bonds, the natural polymer cassava starch is used to make super water absorbents (SWA), 1kg of which can absorb and hold 200 litres of water and release it slowly over time. Placed in the soil near plants’ roots, SWA can be used where there is little rain or frequent drought. After 9 months the crystals, which resemble sugar crystals, completely disintegrate, leaving no residue. (author)

  17. Development of Cosmeceuticals from Natural Products using Proton Beam

    International Nuclear Information System (INIS)

    Lee, G. D.; Choi, J. K.; Hwang, Y. H.

    2009-04-01

    In this report, securing the plant material from the natural substance by irradiating of proton beam, development of the fittest combining and preparing technic for their development of the functional whitening cosmetics, examining the effect of cosmetics developed, safety test of cosmetics developed, stability test of cosmetics, safety test of cosmetics, cytotoxicity test of the cosmetic, and valuation of effect test as a Cosmeceuticals are included

  18. Natural Product Shows Effectiveness in Combating Colorectal Cancer | Poster

    Science.gov (United States)

    An herbal extract used for centuries to prevent heart disease has now been shown to be effective against colorectal cancer when tested in laboratory cell cultures. Scientists from NCI at Frederick found that the natural extract cryptotanshinone (CPT) stops the uncontrolled cell growth characteristic of cancer by interfering with a protein that has been implicated in several cancers, including those of the colon and rectum. The results appear in the journal Molecular and Cellular Biochemistry.

  19. Development of Cosmeceuticals from Natural Products using Proton Beam

    Energy Technology Data Exchange (ETDEWEB)

    Lee, G. D.; Choi, J. K.; Hwang, Y. H. [Dongguk University, Seoul (Korea, Republic of)

    2009-04-15

    In this report, securing the plant material from the natural substance by irradiating of proton beam, development of the fittest combining and preparing technic for their development of the functional whitening cosmetics, examining the effect of cosmetics developed, safety test of cosmetics developed, stability test of cosmetics, safety test of cosmetics, cytotoxicity test of the cosmetic, and valuation of effect test as a Cosmeceuticals are included.

  20. Marine natural products : integrated spectroscopic solutions for structure elucidation

    OpenAIRE

    Morinaka, Brandon Isamu

    2011-01-01

    This dissertation describes the structure determination and biological activity of four groups of sponge derived secondary metabolites by integrated approaches using synthesis and circular dichroism. Chapter 2 describes the isolation and structure determination of brominated ene- yne tetrahydrofuran fatty acids, mutafurans A-G from the marine sponge Xestospongia muta. Two optically active ene- yne tetrahydrofuran model compounds were synthesized for chiroptical comparison to the natural produ...

  1. ON JUSTIFICATION OF STANDARDS FOR NATURAL RADIONUCLIDE CONCENTRATION IN FACING PRODUCTS AND MATERIALS

    Directory of Open Access Journals (Sweden)

    I. P. Stamat

    2009-01-01

    Full Text Available The article addresses issues of the population radiation protection regulatory framework development for the natural sources of radiation. Calculations for justification of standard for natural radionuclide concentration in the wide range of contemporary building materials - facing products - are formulated. The basic consideration of calculations is that implementation of these products could lead to the additional population exposure from natural sources less than 0,1 mSv/year. On the base of this assumption it is shown that effective specific activity of natural radionuclides in these products must not exceed 740 Bq/kg.

  2. The encounter and analysis of naturally occurring radionuclides in gas and oil production and processing

    International Nuclear Information System (INIS)

    Hartog, F.A.; Jonkers, G.; Knaepen, W.A.I.

    1996-01-01

    As a result of oil and gas production, radioactive daughter elements from the uranium and thorium decay series can be mobilized and transported away from the reservoir. Due to changes in flow regime, temperature, pressure or chemical environment NORs (Naturally Occurring Radionuclides) may build up in products, by-products or waste streams from gas and oil production and processing facilities. Products containing NORs are commonly denoted by the acronym NORM (Naturally Occurring Radioactive Materials). Main topics of this paper are: E and P (Exploration and Production) NORM characteristics; incentives for NORM analysis; NORM analysis; interlaboratory test programme; analysis techniques; results and conclusions of the test programme. 4 figs., 2 tabs

  3. Copy, edit, and paste: natural product approaches to biomaterials and neuroengineering.

    Science.gov (United States)

    Gademann, Karl

    2015-03-17

    Progress in the chemical sciences has formed the world we live in, both on a macroscopic and on a nanoscopic scale. The last century witnessed the development of high performance materials that interact with humans on many layers, from clothing to construction, from media to medical devices. On a molecular level, natural products and their derivatives influence many biological processes, and these compounds have enormously contributed to the health and quality of living of humans. Although coatings of stone materials with oils or resins (containing natural products) have led to improved tools already millennia ago, in contrast today, natural product approaches to designer materials, that is, combining the best of both worlds, remain scarce. In this Account, we will summarize our recent research efforts directed to the generation of natural product functionalized materials, exploiting the strategy of "copy, edit, and paste with natural products". Natural products embody the wisdom of evolution, and only total synthesis is able to unlock the secrets enshrined in their molecular structure. We employ total synthesis ("copy") as a scientific approach to address problems related to molecular structure, the biosynthesis of natural products, and their bioactivity. Additionally, the fundamental desire to investigate the mechanism of action of natural products constitutes a key driver for scientific inquiry. In an emerging area of relevance to society, we have prepared natural products such as militarinone D that can stimulate neurite outgrowth and facilitate nerve regeneration. This knowledge obtained by synthetic organic chemistry on complex natural products can then be used to design structurally simplified compounds that retain the biological power of the parent natural product ("edit"). This process, sometimes referred to as function-oriented synthesis, allows obtaining derivatives with better properties, improving their chemical tractability and reducing the step count

  4. Natural product drug discovery: the successful optimization of ISP-1 and halichondrin B.

    Science.gov (United States)

    Yeung, Bryan K S

    2011-08-01

    The concept that natural products provide excellent leads for drug discovery, ultimately producing viable drugs, is a widely accepted view. Natural products embody inherent structural complexity and biological activity which often leads to new targets, pathways, or modes of action. The challenge lies in identifying quality natural product scaffolds that can ultimately result in a drug. Two recently approved drugs originating from unlikely natural product leads, ISP-1 and halichondrin B, were examples of such high quality scaffolds. In initial testing, both compounds displayed excellent in vitro potency, but more importantly were amenable to chemical optimization. This combination of unique biological activity plus the generation of structural activity relationships (SAR) may be early indicators of a high quality natural product scaffold worthy of additional studies. Copyright © 2011 Elsevier Ltd. All rights reserved.

  5. Podoverine A--a novel microtubule destabilizing natural product from the Podophyllum species.

    Science.gov (United States)

    Tran, Tuyen Thi Ngoc; Gerding-Reimers, Claas; Schölermann, Beate; Stanitzki, Bettina; Henkel, Thomas; Waldmann, Herbert; Ziegler, Slava

    2014-09-15

    Natural products represent compound classes with high chemical and structural diversity and various biological activities. Libraries based on natural products are valuable starting point in the search for novel biologically active substances. Here we report on the identification of the natural product podoverine A from the plant Podophyllum versipelle Hance as a novel tubulin-acting agent. A natural product compound collection was subjected to a high-content screen that monitors changes in cytoskeleton and DNA and podoverine A was identified as inhibitor of mitosis. This natural product causes mitotic arrest and inhibits microtubule polymerization in vitro and in cells by targeting the vinca binding site on tubulin. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Feldspar tunneling by Fungi along natural productivity gradients

    NARCIS (Netherlands)

    Hoffland, E.; Giesler, R.; Jongmans, A.G.; Breemen, van N.

    2003-01-01

    Recently, it was hypothesized that ectomycorrhizas hyphae are involved in mineral tunneling. We evaluated the role of ectomycorrhizas in mineral weathering and the ecosystem influx of basic cations by correlating mineral tunnel density to ectomycorrhizas density in two forest productivity gradients.

  7. METHANOL PRODUCTION FROM BIOMASS AND NATURAL GAS AS TRANSPORTATION FUEL

    Science.gov (United States)

    Two processes are examined for production of methanol. They are assessed against the essential requirements of a future alternative fuel for road transport: that it (i) is producible in amounts comparable to the 19 EJ of motor fuel annually consumed in the U.S., (ii) minimizes em...

  8. Camphor: risks and benefits of a widely used natural product ...

    African Journals Online (AJOL)

    The pharmacology, pharmacokinetics and toxicity of this substance are analyzed, with regard to all the literature available, in order to assess a risk profile and better understand the positive and negative aspects connected with its use. The general conclusion is that the main risks of camphor as a medicinal product are ...

  9. Screening and dereplication of microbial natural products extracts

    DEFF Research Database (Denmark)

    Månsson, Maria; Vynne, Nikolaj Grønnegaard; Wietz, Matthias

    It enabled the grouping of similar strains at species and subspecies level disregarding geographical sampling locations. However, intraspecies differences were still observed. In P. luteoviolacea5 and V. coralliilyticus6 some of the differences were related to the production of antibacterial compounds...

  10. Nigerian Journal of Natural Products and Medicine: Contact

    African Journals Online (AJOL)

    Principal Contact. J. M.Agbedahunsi Ph.D. Editor in Chief Drug Research and Production Unit, Faculty of Pharmacy Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, NIGERIA. Phone: +2348034093594. Fax: +234 36 231 733. Email: jagbedah@oauife.edu.ng ...

  11. Natural deep eutectic solvents and their application in natural product research and development

    NARCIS (Netherlands)

    Dai, yuntao

    2013-01-01

    Natural deep eutectic solvents (NADES) ionic liquids (ILs) and deep eutectic solvents (DES) were proposed by our group to extend the range of ILs and DES, particularly to develop cheap, nontoxic, and low viscosity green solvents, and to apply them in health-related fields. They are liquid

  12. Advancement in bioprocess technology: parallels between microbial natural products and cell culture biologics.

    Science.gov (United States)

    Bandyopadhyay, Arpan A; Khetan, Anurag; Malmberg, Li-Hong; Zhou, Weichang; Hu, Wei-Shou

    2017-05-01

    The emergence of natural products and industrial microbiology nearly eight decades ago propelled an era of bioprocess innovation. Half a century later, recombinant protein technology spurred the tremendous growth of biologics and added mammalian cells to the forefront of industrial producing cells in terms of the value of products generated. This review highlights the process technology of natural products and protein biologics. Despite the separation in time, there is a remarkable similarity in their progression. As the new generation of therapeutics for gene and cell therapy emerges, its process technology development can take inspiration from that of natural products and biologics.

  13. New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.

    Science.gov (United States)

    Luo, Yunzi; Enghiad, Behnam; Zhao, Huimin

    2016-02-01

    Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

  14. Analysis of Specific Features of the Ukrainian Market of Natural Gas Production and Consumption

    Directory of Open Access Journals (Sweden)

    Lelyuk Oleksiy V.

    2013-11-01

    Full Text Available The article provides results of the study of specific features of the Ukrainian market of natural gas production and consumption. It analyses dynamics of the specific weight of Ukraine in general volumes of natural gas consumption in the world, dynamics of natural gas consumption in Ukraine during 1990 – 2012 and dependence of natural gas consumption on GDP volumes by the purchasing power parity. It studies the structure of natural gas consumption by regions in 2012 and sectors of economy, resource base of natural gas in Ukraine and also dynamics of established resources of natural gas in Ukraine and dynamics of natural gas production. It analyses base rates of growth of natural gas resources and production in Ukraine. It considers dynamics of import of natural gas into Ukraine and its import prices and also the structure of natural gas import. It identifies the balance of the natural gas market in Ukraine. On the basis of the conducted analysis the article proves that Ukraine is a gas-deficit country of the world, which depends on natural gas import supplies.

  15. Exploring the production of natural gas through the lenses of the ACEGES model

    International Nuclear Information System (INIS)

    Voudouris, Vlasios; Matsumoto, Ken'ichi; Sedgwick, John; Rigby, Robert; Stasinopoulos, Dimitrios; Jefferson, Michael

    2014-01-01

    Due to the increasing importance of natural gas for modern economic activity, and gas's non-renewable nature, it is extremely important to try to estimate possible trajectories of future natural gas production while considering uncertainties in resource estimates, demand growth, production growth and other factors that might limit production. In this study, we develop future scenarios for natural gas supply using the ACEGES computational laboratory. Conditionally on the currently estimated ultimate recoverable resources, the ‘Collective View’ and ‘Golden Age’ Scenarios suggest that the supply of natural gas is likely to meet the increasing demand for natural gas until at least 2035. The ‘Golden Age’ Scenario suggests significant ‘jumps’ of natural gas production – important for testing the resilience of long-term strategies. - Highlights: • We present the ‘Collective View’ and ‘Golden Age’ Scenarios for natural gas production. • We do not observe any significant supply demand pressure of natural gas until 2035. • We do observe ‘jumps’ in natural gas supply until 2035. • The ACEGES-based scenarios can assess the resilience of longterm strategies

  16. Antiviral Activity of Natural Products Extracted from Marine Organisms

    Directory of Open Access Journals (Sweden)

    Sobia Tabassum

    2011-11-01

    Full Text Available Many epidemics have broken out over the centuries. Hundreds and thousands of humans have died over a disease. Available treatments for infectious diseases have always been limited. Some infections are more deadly than the others, especially viral pathogens. These pathogens have continuously resisted all kinds of medical treatment, due to a need for new treatments to be developed. Drugs are present in nature and are also synthesized in vitro and they help in combating diseases and restoring health. Synthesizing drugs is a hard and time consuming task, which requires a lot of man power and financial aid. However, the natural compounds are just lying around on the earth, may it be land or water. Over a thousand novel compounds isolated from marine organisms are used as antiviral agents. Others are being pharmacologically tested. Today, over forty antiviral compounds are present in the pharmacological market. Some of these compounds are undergoing clinical and pre-clinical stages. Marine compounds are paving the way for a new trend in modern medicine.

  17. [Inspirations from natural products based drug research and development for Chinese medicine research--analysis of natural products recoded in TTD].

    Science.gov (United States)

    Chen, Xiu-Ping; Lu, Jin-Jian; Guo, Jia-Jie; Bao, Jiao-Lin; Xu, Wen-Shan; Ding, Qian; Wang, Yi-Tao

    2012-11-01

    Natural product is an important source of new drug research and development (R&D). Traditional Chinese medicine (TCM) innovation is the key step for its modernization and internationalization. However, due to the complexity of TCM, there are many difficulties and confusions in this process. Target-based drug discovery is the mainstream model and method of R&D. TTD, short for therapeutic target database, is developed by National University of Singapore. Besides a large amount of information on drug targets, the database also contains considerable information related to natural products. This paper briefly introduces the TTD, analyzes the natural products derived drugs/compounds recorded in TTD, which we think might provide some inspiration for the innovation of TCM.

  18. Impediments to Marketing African Natural products From Ghana: Preliminary Results

    OpenAIRE

    Govindasamy, Ramu; Onyango, Benjamin M.; Puduri, Venkata S.; Simon, James E.; Asante-Dartey, Juliana; Arthur, Hanson; Diawuo, Bismarck; Acquaye, Dan

    2006-01-01

    For most of the African countries agriculture still remains the mainstay of the economies supplying both food and incomes via marketable surpluses. However, many odds against agriculture such low productivity, poor prices, and drought among others make it unsustainable. Results thus far show that such dependence has contributed little to neither economic development nor growth. Still many of its people living on and from agriculture remain poor, and are susceptible to hunger and malnutrition....

  19. Novel Artificial Natural Products Against Breast Cancer Through Combinatorial Biosynthesis

    Science.gov (United States)

    2002-07-01

    produced by Saccharothix Staphylococcus aureus and Streptococcus faecalis . Re- aerocolonigenes ATCC39243. Several rebeccamycin beccamycin also inhibits the...a mithramycin producer, was used as the I I source of chromosomal DNA. For sporulation it was grown for 7 days at 30 ’C on plates containing A medium ...36 For protoplast regeneration, the organism was grown on R5 solid medium ,4 and R5A medium was B P B El used as the liquid medium for production.3 6

  20. Global warming, population growth, and natural resources for food production.

    Science.gov (United States)

    Pimentel, D

    1991-01-01

    Destruction of forests and the considerable burning of fossil fuels is directly causing the level of carbon dioxide and other greenhouse gases including methane, carbon monoxide, and nitrous oxide in the atmosphere to rise. Population growth in the US and the world indirectly contributes to this global warming. This has led the majority of scientists interested in weather and climate to predict that the planet's temperature will increase from 1.5 to 4.5 degrees Celsius by 2050. These forecasted climactic changes will most likely strongly affect crop production. Specifically these scientists expect the potential changes in temperature, moisture, carbon dioxide, and pests to decrease food production in North America. The degree of changes hinges on each crop and its environmental needs. If farmers begin using improved agricultural technology, the fall in crop yields can be somewhat counterbalanced. Even without global warming, however, agriculture in North America must embrace sensible ecological resource management practices such as conserving soil, water, energy, and biological resources. These sustainable agricultural practices would serve agriculture, farmers, the environment, and society. Agriculturalists, farmers, and society are already interested in sustainable agriculture. Still scientists must conduct more research on the multiple effects of potential global climate change on many different crops under various environmental conditions and on new technologies that farmers might use in agricultural production. We must cut down our consumption of fossil fuel, reduce deforestation, erase poverty, and protect our soil, water, and biological resources. The most important action we need to take, however, is to check population growth.

  1. Comparative genomics of actinomycetes with a focus on natural product biosynthetic genes.

    Science.gov (United States)

    Doroghazi, James R; Metcalf, William W

    2013-09-11

    Actinomycetes are a diverse group of medically, industrially and ecologically important bacteria, studied as much for the diseases they cause as for the cures they hold. The genomes of actinomycetes revealed that these bacteria have a large number of natural product gene clusters, although many of these are difficult to tie to products in the laboratory. Large scale comparisons of these clusters are difficult to perform due to the presence of highly similar repeated domains in the most common biosynthetic machinery: polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs). We have used comparative genomics to provide an overview of the genomic features of a set of 102 closed genomes from this important group of bacteria with a focus on natural product biosynthetic genes. We have focused on well-represented genera and determine the occurrence of gene cluster families therein. Conservation of natural product gene clusters within Mycobacterium, Streptomyces and Frankia suggest crucial roles for natural products in the biology of each genus. The abundance of natural product classes is also found to vary greatly between genera, revealing underlying patterns that are not yet understood. A large-scale analysis of natural product gene clusters presents a useful foundation for hypothesis formulation that is currently underutilized in the field. Such studies will be increasingly necessary to study the diversity and ecology of natural products as the number of genome sequences available continues to grow.

  2. Comparative genomics of actinomycetes with a focus on natural product biosynthetic genes

    Science.gov (United States)

    2013-01-01

    Background Actinomycetes are a diverse group of medically, industrially and ecologically important bacteria, studied as much for the diseases they cause as for the cures they hold. The genomes of actinomycetes revealed that these bacteria have a large number of natural product gene clusters, although many of these are difficult to tie to products in the laboratory. Large scale comparisons of these clusters are difficult to perform due to the presence of highly similar repeated domains in the most common biosynthetic machinery: polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs). Results We have used comparative genomics to provide an overview of the genomic features of a set of 102 closed genomes from this important group of bacteria with a focus on natural product biosynthetic genes. We have focused on well-represented genera and determine the occurrence of gene cluster families therein. Conservation of natural product gene clusters within Mycobacterium, Streptomyces and Frankia suggest crucial roles for natural products in the biology of each genus. The abundance of natural product classes is also found to vary greatly between genera, revealing underlying patterns that are not yet understood. Conclusions A large-scale analysis of natural product gene clusters presents a useful foundation for hypothesis formulation that is currently underutilized in the field. Such studies will be increasingly necessary to study the diversity and ecology of natural products as the number of genome sequences available continues to grow. PMID:24020438

  3. The utility of N-15 nuclear magnetic resonance spectroscopy for the study of natural products

    International Nuclear Information System (INIS)

    Randall, E.W.

    1978-01-01

    The utility of 15 N NMR spectroscopy for the study of natural products and the difficulties which must be overcome arte discussed. The widespread use of pulse Fourier techniques, decouplings, larger magnetic fields and large tube sizes allows a large number of 15 N studies of natural products, the more recent and important of these being peptides, nucleosides and nucleotides. Sites of protonation, tautomerism, sites of nitrosation and proton exchange behaviour for some of these natrual products have been studied. (A.G.)

  4. Mycolic Acid-Containing Bacteria Induce Natural-Product Biosynthesis in Streptomyces Species▿ †

    Science.gov (United States)

    Onaka, Hiroyasu; Mori, Yukiko; Igarashi, Yasuhiro; Furumai, Tamotsu

    2011-01-01

    Natural products produced by microorganisms are important starting compounds for drug discovery. Secondary metabolites, including antibiotics, have been isolated from different Streptomyces species. The production of these metabolites depends on the culture conditions. Therefore, the development of a new culture method can facilitate the discovery of new natural products. Here, we show that mycolic acid-containing bacteria can influence the biosynthesis of cryptic natural products in Streptomyces species. The production of red pigment by Streptomyces lividans TK23 was induced by coculture with Tsukamurella pulmonis TP-B0596, which is a mycolic acid-containing bacterium. Only living cells induced this pigment production, which was not mediated by any substances. T. pulmonis could induce natural-product synthesis in other Streptomyces strains too: it altered natural-product biosynthesis in 88.4% of the Streptomyces strains isolated from soil. The other mycolic acid-containing bacteria, Rhodococcus erythropolis and Corynebacterium glutamicum, altered biosynthesis in 87.5 and 90.2% of the Streptomyces strains, respectively. The coculture broth of T. pulmonis and Streptomyces endus S-522 contained a novel antibiotic, which we named alchivemycin A. We concluded that the mycolic acid localized in the outer cell layer of the inducer bacterium influences secondary metabolism in Streptomyces, and this activity is a result of the direct interaction between the mycolic acid-containing bacteria and Streptomyces. We used these results to develop a new coculture method, called the combined-culture method, which facilitates the screening of natural products. PMID:21097597

  5. Natural selection on floral volatile production in Penstemon digitalis

    Science.gov (United States)

    Parachnowitsch, Amy L.; Burdon, Rosalie C. F.; Raguso, Robert A.; Kessler, André

    2013-01-01

    Natural selection is thought to have shaped the evolution of floral scent; however, unlike other floral characters, we have a rudimentary knowledge of how phenotypic selection acts on scent. We found that floral scent was under stronger selection than corolla traits such as flower size and flower color in weakly scented Penstemon digitalis. Our results suggest that to understand evolution in floral phenotypes, including scent in floral selection, studies are crucial. For P. digitalis, linalool was the direct target of selection in the scent bouquet. Therefore, we determined the enantiomeric configuration of linalool because interacting insects may perceive the enantiomers differentially. We found that P. digitalis produces only (S)-(+)-linalool and, more interestingly, it is also taken up into the nectar. Because the nectar is scented and flavored with (S)-(+)-linalool, it may be an important cue for pollinators visiting P. digitalis flowers. PMID:23221753

  6. Benchmarking of Processes for the Biosynthesis of Natural Products

    DEFF Research Database (Denmark)

    Seita, Catarina Sanches

    of evaluation tools to assess processes both from an economic and environmental perspectives and it is demonstrated with two case-studies. For each case-study different tools are used to evaluate the process. The first case-study consists of the bioconversion of (R)-limonene to (R)-perillic acid by Pseudomonas...... putida GS1. (R)-perillic acid is a monoterpenoic acid with antimicrobial properties. It has a strong inhibitory effect on bacteria and fungus, which makes it an attractive compound to be used as a preservative for instance in cosmetic industry, but on the other hand makes the biosynthesis a complicated......, brazzein, by fermentation of the yeast Pichia pastoris. Brazzein is a natural sweetener found in the fruits of the African plant Pentadiplandra brazzena Baillon, which is sweeter than regular sucrose by several orders of magnitude. Here different tools were applied to evaluate the environmental profile...

  7. Insects: an underrepresented resource for the discovery of biologically active natural products

    Directory of Open Access Journals (Sweden)

    Lauren Seabrooks

    2017-07-01

    Full Text Available Nature has been the source of life-changing and -saving medications for centuries. Aspirin, penicillin and morphine are prime examples of Nature׳s gifts to medicine. These discoveries catalyzed the field of natural product drug discovery which has mostly focused on plants. However, insects have more than twice the number of species and entomotherapy has been in practice for as long as and often in conjunction with medicinal plants and is an important alternative to modern medicine in many parts of the world. Herein, an overview of current traditional medicinal applications of insects and characterization of isolated biologically active molecules starting from approximately 2010 is presented. Insect natural products reviewed were isolated from ants, bees, wasps, beetles, cockroaches, termites, flies, true bugs, moths and more. Biological activities of these natural products from insects include antimicrobial, antifungal, antiviral, anticancer, antioxidant, anti-inflammatory and immunomodulatory effects.

  8. Atmospheric emissions and air quality impacts from natural gas production and use.

    Science.gov (United States)

    Allen, David T

    2014-01-01

    The US Energy Information Administration projects that hydraulic fracturing of shale formations will become a dominant source of domestic natural gas supply over the next several decades, transforming the energy landscape in the United States. However, the environmental impacts associated with fracking for shale gas have made it controversial. This review examines emissions and impacts of air pollutants associated with shale gas production and use. Emissions and impacts of greenhouse gases, photochemically active air pollutants, and toxic air pollutants are described. In addition to the direct atmospheric impacts of expanded natural gas production, indirect effects are also described. Widespread availability of shale gas can drive down natural gas prices, which, in turn, can impact the use patterns for natural gas. Natural gas production and use in electricity generation are used as a case study for examining these indirect consequences of expanded natural gas availability.

  9. Natural radiation - a perspective to radiological risk factors of nuclear energy production

    DEFF Research Database (Denmark)

    Mustonen, R.; Christensen, T.; Stranden, E.

    1992-01-01

    Radiation doses from natural radiation and from man-made modifications on natural radiation, and different natural radiological environments in the Nordic countries are summarized and used as a perspective for the radiological consequences of nuclear energy production. The significance of different...... radiation sources can be judged against the total collective effective dose equivalent from natural radiation in the Nordic countries, 92 000 manSv per year. The collective dose from nuclear energy production during normal operation is estimated to 20 manSv per year and from non-nuclear energy production...... of radionuclides from a final repository of spent nuclear fuel to the consequences of natural radionuclides in different environments. The danger index of natural radiological environments will not be significantly increased by future releases of nuclear fuel radionuclides....

  10. Catalyst-controlled oligomerization for the collective synthesis of polypyrroloindoline natural products

    Science.gov (United States)

    Jamison, Christopher R.; Badillo, Joseph J.; Lipshultz, Jeffrey M.; Comito, Robert J.; MacMillan, David W. C.

    2017-12-01

    In nature, many organisms generate large families of natural product metabolites that have related molecular structures as a means to increase functional diversity and gain an evolutionary advantage against competing systems within the same environment. One pathway commonly employed by living systems to generate these large classes of structurally related families is oligomerization, wherein a series of enzymatically catalysed reactions is employed to generate secondary metabolites by iteratively appending monomers to a growing serial oligomer chain. The polypyrroloindolines are an interesting class of oligomeric natural products that consist of multiple cyclotryptamine subunits. Herein we describe an iterative application of asymmetric copper catalysis towards the synthesis of six distinct oligomeric polypyrroloindoline natural products: hodgkinsine, hodgkinsine B, idiospermuline, quadrigemine H and isopsychotridine B and C. Given the customizable nature of the small-molecule catalysts employed, we demonstrate that this strategy is further amenable to the construction of quadrigemine H-type alkaloids not isolated previously from natural sources.

  11. Generate a bioactive natural product library by mining bacterial cytochrome P450 patterns.

    Science.gov (United States)

    Liu, Xiangyang

    2016-06-01

    The increased number of annotated bacterial genomes provides a vast resource for genome mining. Several bacterial natural products with epoxide groups have been identified as pre-mRNA spliceosome inhibitors and antitumor compounds through genome mining. These epoxide-containing natural products feature a common biosynthetic characteristic that cytochrome P450s (CYPs) and its patterns such as epoxidases are employed in the tailoring reactions. The tailoring enzyme patterns are essential to both biological activities and structural diversity of natural products, and can be used for enzyme pattern-based genome mining. Recent development of direct cloning, heterologous expression, manipulation of the biosynthetic pathways and the CRISPR-CAS9 system have provided molecular biology tools to turn on or pull out nascent biosynthetic gene clusters to generate a microbial natural product library. This review focuses on a library of epoxide-containing natural products and their associated CYPs, with the intention to provide strategies on diversifying the structures of CYP-catalyzed bioactive natural products. It is conceivable that a library of diversified bioactive natural products will be created by pattern-based genome mining, direct cloning and heterologous expression as well as the genomic manipulation.

  12. Strain prioritization for natural product discovery by a high-throughput real-time PCR method.

    Science.gov (United States)

    Hindra; Huang, Tingting; Yang, Dong; Rudolf, Jeffrey D; Xie, Pengfei; Xie, Guangbo; Teng, Qihui; Lohman, Jeremy R; Zhu, Xiangcheng; Huang, Yong; Zhao, Li-Xing; Jiang, Yi; Duan, Yanwen; Shen, Ben

    2014-10-24

    Natural products offer unmatched chemical and structural diversity compared to other small-molecule libraries, but traditional natural product discovery programs are not sustainable, demanding too much time, effort, and resources. Here we report a strain prioritization method for natural product discovery. Central to the method is the application of real-time PCR, targeting genes characteristic to the biosynthetic machinery of natural products with distinct scaffolds in a high-throughput format. The practicality and effectiveness of the method were showcased by prioritizing 1911 actinomycete strains for diterpenoid discovery. A total of 488 potential diterpenoid producers were identified, among which six were confirmed as platensimycin and platencin dual producers and one as a viguiepinol and oxaloterpin producer. While the method as described is most appropriate to prioritize strains for discovering specific natural products, variations of this method should be applicable to the discovery of other classes of natural products. Applications of genome sequencing and genome mining to the high-priority strains could essentially eliminate the chance elements from traditional discovery programs and fundamentally change how natural products are discovered.

  13. Natural product-likeness score revisited: an open-source, open-data implementation.

    Science.gov (United States)

    Jayaseelan, Kalai Vanii; Moreno, Pablo; Truszkowski, Andreas; Ertl, Peter; Steinbeck, Christoph

    2012-05-20

    Natural product-likeness of a molecule, i.e. similarity of this molecule to the structure space covered by natural products, is a useful criterion in screening compound libraries and in designing new lead compounds. A closed source implementation of a natural product-likeness score, that finds its application in virtual screening, library design and compound selection, has been previously reported by one of us. In this note, we report an open-source and open-data re-implementation of this scoring system, illustrate its efficiency in ranking small molecules for natural product likeness and discuss its potential applications. The Natural-Product-Likeness scoring system is implemented as Taverna 2.2 workflows, and is available under Creative Commons Attribution-Share Alike 3.0 Unported License at http://www.myexperiment.org/packs/183.html. It is also available for download as executable standalone java package from http://sourceforge.net/projects/np-likeness/under Academic Free License. Our open-source, open-data Natural-Product-Likeness scoring system can be used as a filter for metabolites in Computer Assisted Structure Elucidation or to select natural-product-like molecules from molecular libraries for the use as leads in drug discovery.

  14. Natural product-likeness score revisited: an open-source, open-data implementation

    Directory of Open Access Journals (Sweden)

    Jayaseelan Kalai Vanii

    2012-05-01

    Full Text Available Abstract Background Natural product-likeness of a molecule, i.e. similarity of this molecule to the structure space covered by natural products, is a useful criterion in screening compound libraries and in designing new lead compounds. A closed source implementation of a natural product-likeness score, that finds its application in virtual screening, library design and compound selection, has been previously reported by one of us. In this note, we report an open-source and open-data re-implementation of this scoring system, illustrate its efficiency in ranking small molecules for natural product likeness and discuss its potential applications. Results The Natural-Product-Likeness scoring system is implemented as Taverna 2.2 workflows, and is available under Creative Commons Attribution-Share Alike 3.0 Unported License at http://www.myexperiment.org/packs/183.html. It is also available for download as executable standalone java package from http://sourceforge.net/projects/np-likeness/under Academic Free License. Conclusions Our open-source, open-data Natural-Product-Likeness scoring system can be used as a filter for metabolites in Computer Assisted Structure Elucidation or to select natural-product-like molecules from molecular libraries for the use as leads in drug discovery.

  15. Web search and data mining of natural products and their bioactivities in PubChem.

    Science.gov (United States)

    Ming, Hao; Tiejun, Cheng; Yanli, Wang; Stephen, Bryant H

    2013-10-01

    Natural products, as major resources for drug discovery historically, are gaining more attentions recently due to the advancement in genomic sequencing and other technologies, which makes them attractive and amenable to drug candidate screening. Collecting and mining the bioactivity information of natural products are extremely important for accelerating drug development process by reducing cost. Lately, a number of publicly accessible databases have been established to facilitate the access to the chemical biology data for small molecules including natural products. Thus, it is imperative for scientists in related fields to exploit these resources in order to expedite their researches on natural products as drug leads/candidates for disease treatment. PubChem, as a public database, contains large amounts of natural products associated with bioactivity data. In this review, we introduce the information system provided at PubChem, and systematically describe the applications for a set of PubChem web services for rapid data retrieval, analysis, and downloading of natural products. We hope this work can serve as a starting point for the researchers to perform data mining on natural products using PubChem.

  16. Omics-based natural product discovery and the lexicon of genome mining.

    Science.gov (United States)

    Machado, Henrique; Tuttle, Robert N; Jensen, Paul R

    2017-10-01

    Genome sequencing and the application of omic techniques are driving many important advances in the field of microbial natural products research. Despite these gains, there remain aspects of the natural product discovery pipeline where our knowledge remains poor. These include the extent to which biosynthetic gene clusters are transcriptionally active in native microbes, the temporal dynamics of transcription, translation, and natural product assembly, as well as the relationships between small molecule production and detection. Here we touch on a number of these concepts in the context of continuing efforts to unlock the natural product potential revealed in genome sequence data and discuss nomenclatural issues that warrant consideration as the field moves forward. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Scaffold Diversity Inspired by the Natural Product Evodiamine: Discovery of Highly Potent and Multitargeting Antitumor Agents.

    Science.gov (United States)

    Wang, Shengzheng; Fang, Kun; Dong, Guoqiang; Chen, Shuqiang; Liu, Na; Miao, Zhenyuan; Yao, Jianzhong; Li, Jian; Zhang, Wannian; Sheng, Chunquan

    2015-08-27

    A critical question in natural product-based drug discovery is how to translate the product into drug-like molecules with optimal pharmacological properties. The generation of natural product-inspired scaffold diversity is an effective but challenging strategy to investigate the broader chemical space and identify promising drug leads. Extending our efforts to the natural product evodiamine, a diverse library containing 11 evodiamine-inspired novel scaffolds and their derivatives were designed and synthesized. Most of them showed good to excellent antitumor activity against various human cancer cell lines. In particular, 3-chloro-10-hydroxyl thio-evodiamine (66c) showed excellent in vitro and in vivo antitumor efficacy with good tolerability and low toxicity. Antitumor mechanism and target profiling studies indicate that compound 66c is the first-in-class triple topoisomerase I/topoisomerase II/tubulin inhibitor. Overall, this study provided an effective strategy for natural product-based drug discovery.

  18. Transporter-mediated natural product-drug interactions for the treatment of cardiovascular diseases.

    Science.gov (United States)

    Zha, Weibin

    2018-04-01

    The growing use of natural products in cardiovascular (CV) patients has been greatly raising the concerns about potential natural product-CV drug interactions. Some of these may lead to unexpected cardiovascular adverse effects and it is, therefore, essential to identify or predict potential natural product-CV drug interactions, and to understand the underlying mechanisms. Drug transporters are important determinants for the pharmacokinetics of drugs and alterations of drug transport has been recognized as one of the major causes of natural product-drug interactions. In last two decades, many CV drugs (e.g., angiotensin II receptor blockers, beta-blockers and statins) have been identified to be substrates and inhibitors of the solute carrier (SLC) transporters and the ATP-binding cassette (ABC) transporters, which are two major transporter superfamilies. Meanwhile, in vitro and in vivo studies indicate that a growing number of natural products showed cardioprotective effects (e.g., gingko biloba, danshen and their active ingredients) are also substrates and inhibitors of drug transporters. Thus, to understand transporter-mediated natural product-CV drug interactions is important and some transporter-mediated interactions have already shown to have clinical relevance. In this review, we review the current knowledge on the role of ABC and SLC transporters in CV therapy, as well as transporter modulation by natural products used in CV diseases and their induced natural product-CV drug interactions through alterations of drug transport. We hope our review will aid in a comprehensive summary of transporter-mediated natural product-CV drug interactions and help public and physicians understand these type of interactions. Copyright © 2017. Published by Elsevier B.V.

  19. Natural products used as a chemical library for protein-protein interaction targeted drug discovery.

    Science.gov (United States)

    Jin, Xuemei; Lee, Kyungro; Kim, Nam Hee; Kim, Hyun Sil; Yook, Jong In; Choi, Jiwon; No, Kyoung Tai

    2018-01-01

    Protein-protein interactions (PPIs), which are essential for cellular processes, have been recognized as attractive therapeutic targets. Therefore, the construction of a PPI-focused chemical library is an inevitable necessity for future drug discovery. Natural products have been used as traditional medicines to treat human diseases for millennia; in addition, their molecular scaffolds have been used in diverse approved drugs and drug candidates. The recent discovery of the ability of natural products to inhibit PPIs led us to use natural products as a chemical library for PPI-targeted drug discovery. In this study, we collected natural products (NPDB) from non-commercial and in-house databases to analyze their similarities to small-molecule PPI inhibitors (iPPIs) and FDA-approved drugs by using eight molecular descriptors. Then, we evaluated the distribution of NPDB and iPPIs in the chemical space, represented by the molecular fingerprint and molecular scaffolds, to identify the promising scaffolds, which could interfere with PPIs. To investigate the ability of natural products to inhibit PPI targets, molecular docking was used. Then, we predicted a set of high-potency natural products by using the iPPI-likeness score based on a docking score-weighted model. These selected natural products showed high binding affinities to the PPI target, namely XIAP, which were validated in an in vitro experiment. In addition, the natural products with novel scaffolds might provide a promising starting point for further medicinal chemistry developments. Overall, our study shows the potency of natural products in targeting PPIs, which might help in the design of a PPI-focused chemical library for future drug discovery. Copyright © 2017 Elsevier Inc. All rights reserved.

  20. Saffron: a natural product with potential pharmaceutical applications.

    Science.gov (United States)

    Christodoulou, Eirini; Kadoglou, Nikolaos P E; Kostomitsopoulos, Nikolaos; Valsami, Georgia

    2015-12-01

    Recently, a great deal of interest has been developed to isolate and investigate novel bioactive components from natural resources with health beneficial effects. Saffron is the dried stigma of Crocus sativus L. and has been used for centuries in traditional medicine mainly for its healing properties, as well as for the treatment of various pathological conditions. Objectives of the present review are to unravel its therapeutic properties and investigate the potential applications of saffron in contemporary therapy of a wide spectrum of diseases and summarize previous and current evidence regarding the biological/pharmacological activities of saffron and its active ingredients and their possible therapeutic uses. Recent phytochemistry and pharmacological experiments have indicated that crocin and safranal, the major active ingredients of saffron, exert important actions, such as antioxidant, anti-tumor, anti-diabetic, anti-inflammatory and anti-atherosclerotic. Unfortunately, the vast majority of those data derive from in vitro studies, whereas a limited number of in vivo experiments support the aforementioned effects. In addition to studies with mechanistic implications, very few clinical trials provide preliminary evidence of saffron potentiality to alleviate depression and increase cognitive function in patients with Alzheimer's disease. The history and structural features of saffron constituents are given in the first part of the review, followed by a comprehensive and critical presentation of the published preclinical and clinical studies and review papers on the pharmacology and possible therapeutic uses of saffron and its main active components crocin and safranal. © 2015 Royal Pharmaceutical Society.

  1. Antimicrobial effect of natural dyeing products upon decaycausing bacteria

    Directory of Open Access Journals (Sweden)

    Mayara Abreu Pinheiro

    2012-06-01

    Full Text Available Objective: To evaluate the bacteriostatic and bactericidal activities of tinctures of Rosmarinus officinalis (Rosemary, Calendula officinalis (Marigold and Mikania glomerata (Guaco upon decay-causing bacteria and compare them to chlorhexidine’s. Methods: The Minimum Inhibitory Concentration (MIC was determined by microdilution technique, using 96-well microplates. In each well were inserted 10μL of inoculum (108 cells/mL, 100μL of doubly concentrated BHI broth and 100μL of tinctures at concentrations that vary between 100 and 0.78 mg/mL for T1, T2 and T3. As positive control, Chlorhexidine 0.12% was used. Reading was performed after 24 hours, by visual method. The assays were realized in triplicate. Minimum Bactericidal Concentration (MBC was obtained by seeding of last bacteriostatic and its preceding concentrations, for each product, on Blood Agar. The MBC was considered in the plates that did not present any bacterial growth. Results: Upon Streptococcus mutans, MIC was verified at 6.25mg/mL, for T1, T2, and T3 and MBC at 12.5mg/mL for T1 and T2, and at 6.25mg/mL for T3. Upon Streptococcus oralis, all products presented MIC and MBC at 0.78mg/mL. For Chlorhexidine, the MIC and MBC were 0,04mg/mL, upon S. mutans and S. oralis. Conclusion: The tinctures of Rosemary, Marigold and Guaco showed bactericidal and bacteriostatic activities at low concentrations, upon Streptococcus mutans e Streptococcus oralis. However, the antimicrobial activity of chlorhexidine was superior to the tinctures’

  2. Animal-derived natural products review: focus on novel modifications and applications.

    Science.gov (United States)

    Fan, Qianqian; Ma, Jianzhong; Xu, Qunna; Zhang, Jing; Simion, Demetra; Carmen, Gaidău; Guo, Congsheng

    2015-04-01

    Bio-based natural products have attracted exploding interests, while the environmental pollutions caused by the synthetic polymers are deteriorating dramatically. In this review, we provide a comprehensive overview of the modification of animal-derived natural products with an emphasis on casein, chitosan and collagen. Furthermore, their novel applications in controlled drug delivery system, leather finishing, and pollutant adsorption are also demonstrated. Accordingly, some perspectives in the future development of animal-derived natural products are further proposed. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Synthetic Strategies toward Natural Products Containing Contiguous Stereogenic Quaternary Carbon Atoms.

    Science.gov (United States)

    Büschleb, Martin; Dorich, Stéphane; Hanessian, Stephen; Tao, Daniel; Schenthal, Kyle B; Overman, Larry E

    2016-03-18

    Strategies for the total synthesis of complex natural products that contain two or more contiguous stereogenic quaternary carbon atoms in their intricate structures are reviewed with 12 representative examples. Emphasis has been put on methods to create quaternary carbon stereocenters, including syntheses of the same natural product by different groups, thereby showcasing the diversity of thought and individual creativity. A compendium of selected natural products containing two or more contiguous stereogenic quaternary carbon atoms and key reactions in their total or partial syntheses is provided in the Supporting Information. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Polycarbonates from the polyhydroxy natural product quinic acid.

    Science.gov (United States)

    Besset, Céline J; Lonnecker, Alexander T; Streff, Jennifer M; Wooley, Karen L

    2011-07-11

    Strategies for the preparation of polycarbonates, derived from natural polyhydroxy monomeric repeat units, were developed for biosourced polycarbonates based on quinic acid. The design and synthesis of regioselectively tert-butyldimethylsilyloxy (TBS)-protected 1,4- and 1,5-diol monomers of quinic acid were followed by optimization of their copolymerizations with phosgene, generated in situ from trichloromethyl chloroformate, to yield protected poly(1,4-quinic acid carbonate) and poly(1,5-quinic acid carbonate). The molecular weights reached ca. 7.6 kDa, corresponding to degrees of polymerization of ca. 24, with polydispersities ranging from 2.0 to 3.5, as measured by SEC using tetrahydrofuran as the eluent and with polystyrene calibration standards. Partially because of the presence of the bicyclic backbone, each regioisomeric poly(quinic acid carbonate) exhibited relatively high glass-transition temperatures, 209 °C for poly(1,4-quinic acid carbonate) and 229 °C for poly(1,5-quinic acid carbonate). Removal of the TBS-protecting groups was studied under mild conditions to achieve control over potential competing reactions involving polymer degradation, which could include cleavage of lactones within the repeat units, carbonate linkages, or both between the repeat units. Full deprotection was not achieved without some degree of polymer degradation. The regiochemistry of the monomer showed significant impact on the reactivity during deprotection and also on the thermal properties, with the 1,5-regioisomeric polymer having lower reactivity and giving higher T(g) values, in comparison with the 1,4-regioisomer. Each regioisomer underwent a 10-20 °C increase in T(g) upon partial removal of the TBS-protecting groups. As the extent of deprotection increased, the solubility decreased. Ultimately, at long deprotection reaction times, the solubility increased and the T(g) decreased because of significant degradation of the polymers.

  5. NATURAL PRODUCTS AS PRESERVATIVES FOR FAST GROWTH WOODS - A REVIEW

    Directory of Open Access Journals (Sweden)

    Ricardo Marques Barreiros

    2011-11-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Wood is a universal material, economic, historic and sustainable. The paucity of species resistant to biological degradation forced man to use other less durable, especially fast growing, from reforestation, as some species of Eucalyptus and Pinus. These species have moderate or no resistance to wood decay organisms need special treatment and preservatives. The products currently used preservatives are highly toxic and are potential environmental hazards and human health. Thus, there is a growing need to develop effective chemicals, non-toxic to humans and the environment. The direction of research has aimed to develop environmentally friendly products and economic viability, and an alternative is the use of Crude Tall Oil (CTO, which is a waste processing coniferous softwood pulp for the production of kraft paper. The tall oil as a protective agent, has been considered a promising method for significantly reducing the capillary water absorption of sapwood, thereby removing one of the factors that favor the wood being attacked by fungi and insects: water, oxygen and nutrients. Research shows that the tall oil can be used neat, either fresh or distilled, or in combination with biocides.A madeira é um material universal, econ

  6. Products of steel slags an opportunity to save natural resources.

    Science.gov (United States)

    Motz, H; Geiseler, J

    2001-01-01

    already accepted as a CEN standard and are used for a continuous quality control. Usually the suitability of steel slags is stated by fulfilling the requirements of national and/or international standards and regulations. Based on these standards and regulations in Germany in 1998 about 97% of the produced steel slags have been used as aggregates for road construction (e.g. as surface layer, road base and sub base for high trafficked roads), ways, earthworks, and armourstones for hydraulic structures. Consistent to the successful long-term experience not only products of steel slags but also products of blast furnace slags have been eliminated from the European Waste Catalogue and the European Shipment of Waste Regulation of the European Community, as well as from the lists of OECD for transfrontier movements by the decision of the OECD-Council from 21 September, 1995.

  7. Soil Properties and Plant Biomass Production in Natural Rangeland Management Systems

    Directory of Open Access Journals (Sweden)

    Romeu de Souza Werner

    Full Text Available ABSTRACT Improper management of rangelands can cause land degradation and reduce the economic efficiency of livestock activity. The aim of this study was to evaluate soil properties and quantify plant biomass production in four natural rangeland management systems in the Santa Catarina Plateau (Planalto Catarinense of Brazil. The treatments, which included mowed natural rangeland (NR, burned natural rangeland (BR, natural rangeland improved through the introduction of plant species after harrowing (IH, and natural rangeland improved through the introduction of plant species after chisel plowing (IC, were evaluated in a Nitossolo Bruno (Nitisol. In the improved treatments, soil acidity was corrected, phosphate fertilizer was applied, and intercropped annual ryegrass (Lolium multiflorum, velvet grass (Holcus lanatus, and white clover (Trifolium repens were sown. Management systems with harrowed or chisel plowed soil showed improved soil physical properties; however, the effect decreased over time and values approached those of burned and mowed natural rangelands. Natural rangeland systems in the establishment phase had little influence on soil organic C. The mowed natural rangeland and improved natural rangeland exhibited greater production of grazing material, while burning the field decreased production and increased the proportion of weeds. Improvement of the natural rangelands increased leguminous biomass for pasture.

  8. Expanding Canadian natural gas production will strengthen growth of LP-gas industry

    International Nuclear Information System (INIS)

    Hawkins, D.J.

    1994-01-01

    In 1992, over 86% of Canadian propane and 70% of Canadian butane production originated in gas plants. Propane and butane production not recovered at gas plants is recovered in other processing facilities, primarily refineries and heavy oil upgraders. As a result, supplies of both products are largely tied to natural gas production, and the outlook for natural gas therefore provides the basis for any discussion on the outlook for gas processing and NGL industry infrastructure. The paper discusses gas processing, economies of scale, NGL supply, expected declines, industry structure and infrastructure, the two major centers of the Canadian NGL industry, new shippers, and required pipeline expansion

  9. Investigation on natural radioactive nuclide contents of rock products in Xi'an construction materials market

    International Nuclear Information System (INIS)

    Zhou Chunlin; Han Feng; Shang Aiguo; Li Tiantuo; Guo Huiping; Yie Lichao; Li Guifang

    2001-01-01

    The author reports the investigation results on natural radioactive nuclide contents of rock products from Xi'an construction materials market. The products were classified according to the national standard. The results show that natural radioactive nuclide contents in sampled rock products are in normal radioactive background levels. The radio-activity ranges of 238 U, 226 Ra, 232 Th and 40 K are 2.7 - 181.8, 0.92 - 271.0, 0.63 - 148.0, 1.8 - 1245 Bq·kg -1 , respectively. According to the national standard (JC 518-93), the application of some rock products must be limited

  10. Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification.

    Science.gov (United States)

    Liang, Chaoning; Zhang, Yi; Jia, Yan; Wenzhao Wang; Li, Youhai; Lu, Shikun; Jin, Jian-Ming; Tang, Shuang-Yan

    2016-02-12

    Glycodiversification broadens the scope of natural product-derived drug discovery. The acceptor substrate promiscuity of glucosyltransferase-D (GTF-D), a carbohydrate-processing enzyme from Streptococcus mutans, was expanded by protein engineering. Mutants in a site-saturation mutagenesis library were screened on the fluorescent substrate 4-methylumbelliferone to identify derivatives with improved transglycosylation efficiency. In comparison to the wild-type GTF-D enzyme, mutant M4 exhibited increased transglycosylation capabilities on flavonoid substrates including catechin, genistein, daidzein and silybin, using the glucosyl donor sucrose. This study demonstrated the feasibility of developing natural product glycosyltransferases by engineering transglycosidases that use donor substrates cheaper than NDP-sugars, and gave rise to a series of α-glucosylated natural products that are novel to the natural product reservoir. The solubility of the α-glucoside of genistein and the anti-oxidant capability of the α-glucoside of catechin were also studied.

  11. Ribosome-mediated synthesis of natural product-like peptides via cell-free translation.

    Science.gov (United States)

    Maini, Rumit; Umemoto, Shiori; Suga, Hiroaki

    2016-10-01

    Peptide natural products (PNPs) represent a unique class of compounds known for their fascinating structural motifs with important biological activities. Lately, PNPs have garnered a lot of interest for their application in drug discovery. Nevertheless, lack of diversity oriented synthetic/biosynthetic platforms to generate large natural product-like libraries has limited their development as peptide therapeutics. The promiscuity of cell-free translation has allowed for the synthesis of artificial PNPs having complex structural features. Modified cell-free translation systems coupled with the display technologies have generated diverse natural product-like peptide libraries and led to the discovery of several biologically active molecules. Such technologies have drastically decreased the time to obtain peptide drug leads and therefore, revolutionized the field of peptide drug discovery. In this account, we review recent developments in the synthesis of natural product-like peptides via cell-free translation. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Endophytes : Exploiting biodiversity for the improvement of natural product-based drug discovery

    NARCIS (Netherlands)

    Staniek, Agata; Woerdenbag, Herman J.; Kayser, Oliver

    2008-01-01

    Endophytes, microorganisms that colonize internal tissues of all plant species, create a huge biodiversity with yet unknown novel natural products, presumed to push forward the frontiers of drug discovery. Next to the clinically acknowledged antineoplastic agent, paclitaxel, endophyte research has

  13. Cyclic Sulfamidate Enabled Syntheses of Amino Acids, Peptides, Carbohydrates, and Natural Products

    Science.gov (United States)

    This article reviews the emergence of cyclic sulfamidates as versatile intermediatesfor the synthesis of unnatural amino acids, chalcogen peptides, modified sugars, drugs and drug candidates, and important natural products.

  14. Design of compound libraries based on natural product scaffolds and protein structure similarity clustering (PSSC)

    NARCIS (Netherlands)

    Balamurugan, Rengarajan; Dekker, Frank J; Waldmann, Herbert; Dekker, Frans

    Recent advances in structural biology, bioinformatics and combinatorial chemistry have significantly impacted the discovery of small molecules that modulate protein functions. Natural products which have evolved to bind to proteins may serve as biologically validated starting points for the design

  15. Inhibition of aflatoxin B production of Aspergillus flavus, isolated from soybean seeds by certain natural plant products.

    Science.gov (United States)

    Krishnamurthy, Y L; Shashikala, J

    2006-11-01

    The inhibitory effect of cowdung fumes, Captan, leaf powder of Withania somnifera, Hyptis suaveolens, Eucalyptus citriodora, peel powder of Citrus sinensis, Citrus medica and Punica granatum, neem cake and pongamia cake and spore suspension of Trichoderma harzianum and Aspergillus niger on aflatoxin B(1) production by toxigenic strain of Aspergillus flavus isolated from soybean seeds was investigated. Soybean seed was treated with different natural products and fungicide captan and was inoculated with toxigenic strain of A. flavus and incubated for different periods. The results showed that all the treatments were effective in controlling aflatoxin B(1) production. Captan, neem cake, spore suspension of T. harzianum, A. niger and combination of both reduced the level of aflatoxin B(1) to a great extent. Leaf powder of W. somnifera, H. suaveolens, peel powder of C. sinensis, C. medica and pongamia cake also controlled the aflatoxin B(1) production. All the natural product treatments applied were significantly effective in inhibiting aflatoxin B(1) production on soybean seeds by A. flavus. These natural plant products may successfully replace chemical fungicides and provide an alternative method to protect soybean and other agricultural commodities from aflatoxin B(1) production by A. flavus.

  16. A pilot study to compare natural health product-drug interactions in two databases in Canada.

    Science.gov (United States)

    Faubert, Guillaume; Lebel, Denis; Bussières, Jean-François

    2010-04-01

    The aim of this article is to evaluate and compare two natural health product databases for the purpose of integrating them into a pharmacy information system in Canada. This is a descriptive pilot study that compared the Natural Medicine Comprehensive Database (NMCD) and Natural Standard Database (NSD). We randomly chose five hospital patient files. For each drug prescription, we searched for and counted the number of potential natural health product-drug interactions in each database. We compared all of the potential interactions between dexamethasone and the natural health products in both databases. We also evaluated the quality of a selection of references for 30 potential natural health product-dexamethasone interactions. Five pediatric patient files were selected for a total of 21 different common names. The number of potential natural health product-drug interactions identified varied from 12 (salbutamol-albuterol) to 129 (dexamethasone) in the NSD for an average of 63 +/- 33. The number of potential natural health product-drug interactions identified varied from 1 (salbutamol-albuterol) to 96 (dexamethasone) in the NMCD for an average of 55 +/- 27. There was no significant difference between the average number of potential natural health product-drug interactions between the databases (P = 0.40). The average number of common potential interactions was 9 +/- 8. Thirty potential interactions of dexamethasone were compared. The number of abstracts per pair of interactions varied from 0 (Aloe-dexamethasone in the NSD) to 17 (dexamethasone-St. John's wort in the NMCD). For the 10 pairs that were common to both databases, the number of abstracts given was 4.5 +/- 4.7 for the NMCD and 3.1 +/- 2.1 for the NSD (P = 0.41) and the average evaluation score was 4.6 +/- 1.5 for the NMCD and 5.6 +/- 1.2 for the NSD (P = 0.43). This pilot study presents a comparison of potential natural health product-drug interactions from two different databases using two different

  17. Use of in situ solid-phase adsorption in microbial natural product fermentation development.

    Science.gov (United States)

    Phillips, Thomas; Chase, Matthew; Wagner, Stephanie; Renzi, Chris; Powell, Marcella; DeAngelo, Joseph; Michels, Peter

    2013-05-01

    It has been half a century since investigators first began experimenting with adding ion exchange resins during the fermentation of microbial natural products. With the development of nonionic polymeric adsorbents in the 1970s, the application of in situ product adsorption in bioprocessing has grown slowly, but steadily. To date, in situ product adsorption strategies have been used in biotransformations, plant cell culture, the production of biofuels, and selected bulk chemicals, such as butanol and lactic acid, as well as in more traditional natural product fermentation within the pharmaceutical industry. Apart from the operational gains in efficiency from the integration of fermentation and primary recovery, the addition of adsorbents during fermentation has repeatedly demonstrated the capacity to significantly increase titers by sequestering the product and preventing or mitigating degradation, feedback inhibition and/or cytotoxic effects. Adoption of in situ product adsorption has been particularly valuable in the early stages of natural product-based drug discovery programs, where quickly and cost-effectively generating multigram quantities of a lead compound can be challenging when using a wild-type strain and fermentation conditions that have not been optimized. While much of the literature involving in situ adsorption describes its application early in the drug development process, this does not imply that the potential for scale-up is limited. To date, commercial-scale processes utilizing in situ product adsorption have reached batch sizes of at least 30,000 l. Here we present examples where in situ product adsorption has been used to improve product titers or alter the ratios among biosynthetically related natural products, examine some of the relevant variables to consider, and discuss the mechanisms by which in situ adsorption may impact the biosynthesis of microbial natural products.

  18. Natural products from marine algae of the genus Osmundaria (Rhodophyceae, Ceramiales).

    Science.gov (United States)

    Osako, Kelvin; Teixeira, Valéria Laneuville

    2013-04-01

    The present work is a review of the natural products isolated from red marine algae of the genus Osmundaria (including Vidalia), which intends to encompass their occurrence in the species of this genus, the possible synonymies, their geographic distribution, their structural variety and their biological potential as prototypes for the pharmaceutical industry and as active principles of cosmetics. At the end, we provide a table with these natural products and their biological activities.

  19. Study on preparation of new antioxidants for radiation vulcanized natural rubber latex product. Antioxidant from keratin

    International Nuclear Information System (INIS)

    Nguyen Quoc Hien; Nguyen Van Toan; Vo Tan Thien; Le Hai

    2000-01-01

    The thermo-oxidative aging resistance of radiation vulcanization of natural rubber latex (RVNRL) products should be adequately by using suitable antioxidants or new kind of effective antioxidant. This work presents the results of preparation of natural antioxidant from hair keratin. Characteristics and effectiveness of resultant antioxidant are also presented. The results obtained indicates that antioxidant made from hair keratin is safe and effective for rubber products from RVNRL. (author)

  20. Search for New Bioactive Marine Natural Products and Application to Drug Development.

    Science.gov (United States)

    Kobayashi, Jun'ichi

    2016-01-01

    Natural products are well recognized as an important source of lead compounds in drug development. During the past >30 years, we have discovered >1000 novel bioactive natural products from Okinawan marine organisms (sponges, tunicates, cone shells, etc.) and microorganisms (fungi, bacteria, dinoflagellates, etc.). Some of them are used as bioprobes useful for basic studies of life sciences, while others are expected to be candidates of drug leads.

  1. Collision-Induced Dissociation Mass Spectrometry: A Powerful Tool for Natural Product Structure Elucidation.

    Science.gov (United States)

    Johnson, Andrew R; Carlson, Erin E

    2015-11-03

    Mass spectrometry is a powerful tool in natural product structure elucidation, but our ability to directly correlate fragmentation spectra to these structures lags far behind similar efforts in peptide sequencing and proteomics. Often, manual data interpretation is required and our knowledge of the expected fragmentation patterns for many scaffolds is limited, further complicating analysis. Here, we summarize advances in natural product structure elucidation based upon the application of collision induced dissociation fragmentation mechanisms.

  2. Marine Natural Products from Seaweeds [Produtos Naturais de Algas Marinhas Bentônicas

    OpenAIRE

    Valéria L. Teixeira

    2013-01-01

    Natural products from seaweeds have been studied with the aim of finding new drugs of industrial value, understanding their ecological role, and well as establishing the limits of taxonomic species, genera and families of marine algae. The present review shows the main features of natural products from seaweeds of the Divisions Chlorophyta – Class Ulvophyceae (green seaweeds), Ochrophyta – Class Phaeophyceae (Brown seaweeds) and Rhodophyta (red seaweeds). The most important examples of natura...

  3. Tolerance of natural baby skin-care products on healthy, full-term infants and toddlers

    OpenAIRE

    Coret, Catherine D; Suero, Michael B; Tierney, Neena K

    2014-01-01

    Catherine D Coret, Michael B Suero, Neena K Tierney Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, USA Purpose: To evaluate the tolerance of baby skin-care products with at least 95% naturally derived ingredients on infants and toddlers. Materials and methods: Healthy, full-term infants and toddlers aged 1–36 months were enrolled. In study 1, a lightly fragranced natural baby hair and body wash (n=30), a lightly fragranced natural baby shampoo (n=30), or a lightly...

  4. Productivity as related to diversity and age in planted versus natural forests

    Science.gov (United States)

    Qinfeng Guo; Hai Ren

    2014-01-01

    Little is known about the performance of plantations relative to natural forests of the same climate zone and age. China has more plantations than any other country as a consequence of massive afforestation efforts.We use data from China to comparatively examine tree biomass and productivity of planted and natural stands in relation to climate zone, latitude, elevation...

  5. Natural Product-Derived Spirooxindole Fragments Serve as Privileged Substructures for Discovery of New Anticancer Agents.

    Science.gov (United States)

    Yu, Bin; Zheng, Yi-Chao; Shi, Xiao-Jing; Qi, Ping-Ping; Liu, Hong-Min

    2016-01-01

    The utility of natural products for identifying anticancer agents has been highly pursued in the last decades and over 100 drug molecules in clinic are natural products or natural product-derived compounds. Natural products are believed to be able to cover unexplored chemical space that is normally not occupied by commercially available molecule libraries. However, the low abundance and synthetic intractability of natural products have limited their applications in drug discovery. Recently, the identification of biologically relevant fragments derived from biologically validated natural products has been recognized as a powerful strategy in searching new biological probes and drugs. The spirocyclic oxindoles, as privileged structural scaffolds, have shown their potential in designing new drugs. Several anticancer drug candidates such as SAR405838, RO8994, CFI-400945 and their bioisosteres are undergoing clinical trials or preclinical studies. To highlight the significant progress, we focus on illustrating the discovery of SAR405838, RO8994, CFI-400945 and their bioisosteres for cancer therapy using substructure-based strategies and discussing modes of action, binding models and preclinical data.

  6. Production of natural grassland in the central Orange Free State in ...

    African Journals Online (AJOL)

    In order to plan a livestock enterprise it is imperative to know the production potential of the natural grassland. Grassland production is determined mainly by climate and soil, provided reasonably sound management practices are employed. It is thus evident that effective planning must necessarily depend heavily on a ...

  7. Influence of natural organic matter fouling and osmotic backwash on pressure retarded osmosis energy production from natural salinity gradients.

    Science.gov (United States)

    Yip, Ngai Yin; Elimelech, Menachem

    2013-01-01

    Pressure retarded osmosis (PRO) has the potential to produce clean, renewable energy from natural salinity gradients. However, membrane fouling can lead to diminished water flux productivity, thus reducing the extractable energy. This study investigates organic fouling and osmotic backwash cleaning in PRO and the resulting impact on projected power generation. Fabricated thin-film composite membranes were fouled with model river water containing natural organic matter. The water permeation carried foulants from the feed river water into the membrane porous support layer and caused severe water flux decline of ∼46%. Analysis of the water flux behavior revealed three phases in membrane support layer fouling. Initial foulants of the first fouling phase quickly adsorbed at the active-support layer interface and caused a significantly greater increase in hydraulic resistance than the subsequent second and third phase foulants. The water permeability of the fouled membranes was lowered by ∼39%, causing ∼26% decrease in projected power density. A brief, chemical-free osmotic backwash was demonstrated to be effective in removing foulants from the porous support layer, achieving ∼44% recovery in projected power density. The substantial performance recovery after cleaning was attributed to the partial restoration of the membrane water permeability. This study shows that membrane fouling detrimentally impacts energy production, and highlights the potential strategies to mitigate fouling in PRO power generation with natural salinity gradients.

  8. Structure-Activity Relationships of the Bioactive Thiazinoquinone Marine Natural Products Thiaplidiaquinones A and B

    Directory of Open Access Journals (Sweden)

    Jacquie L. Harper

    2015-08-01

    Full Text Available In an effort to more accurately define the mechanism of cell death and to establish structure-activity relationship requirements for the marine meroterpenoid alkaloids thiaplidiaquinones A and B, we have evaluated not only the natural products but also dioxothiazine regioisomers and two precursor quinones in a range of bioassays. While the natural products were found to be weak inducers of ROS in Jurkat cells, the dioxothiazine regioisomer of thiaplidiaquinone A and a synthetic precursor to thiaplidiaquinone B were found to be moderately potent inducers. Intriguingly, and in contrast to previous reports, the mechanism of Jurkat cell death (necrosis vs. apoptosis was found to be dependent upon the positioning of one of the geranyl sidechains in the compounds with thiaplidiaquinone A and its dioxothiazine regioisomer causing death dominantly by necrosis, while thiaplidiaquinone B and its dioxothiazine isomer caused cell death via apoptosis. The dioxothiazine regioisomer of thiaplidiaquinone A exhibited more potent in vitro antiproliferative activity against human tumor cells, with NCI sub-panel selectivity towards melanoma cell lines. The non-natural dioxothiazine regioisomers were also more active in antiplasmodial and anti-farnesyltransferase assays than their natural product counterparts. The results highlight the important role that natural product total synthesis can play in not only helping understand the structural basis of biological activity of natural products, but also the discovery of new bioactive scaffolds.

  9. Regional air quality impacts of increased natural gas production and use in Texas.

    Science.gov (United States)

    Pacsi, Adam P; Alhajeri, Nawaf S; Zavala-Araiza, Daniel; Webster, Mort D; Allen, David T

    2013-04-02

    Natural gas use in electricity generation in Texas was estimated, for gas prices ranging from $1.89 to $7.74 per MMBTU, using an optimal power flow model. Hourly estimates of electricity generation, for individual electricity generation units, from the model were used to estimate spatially resolved hourly emissions from electricity generation. Emissions from natural gas production activities in the Barnett Shale region were also estimated, with emissions scaled up or down to match demand in electricity generation as natural gas prices changed. As natural gas use increased, emissions decreased from electricity generation and increased from natural gas production. Overall, NOx and SO2 emissions decreased, while VOC emissions increased as natural gas use increased. To assess the effects of these changes in emissions on ozone and particulate matter concentrations, spatially and temporally resolved emissions were used in a month-long photochemical modeling episode. Over the month-long photochemical modeling episode, decreases in natural gas prices typical of those experienced from 2006 to 2012 led to net regional decreases in ozone (0.2-0.7 ppb) and fine particulate matter (PM) (0.1-0.7 μg/m(3)). Changes in PM were predominantly due to changes in regional PM sulfate formation. Changes in regional PM and ozone formation are primarily due to decreases in emissions from electricity generation. Increases in emissions from increased natural gas production were offset by decreasing emissions from electricity generation for all the scenarios considered.

  10. In-use product stocks link manufactured capital to natural capital.

    Science.gov (United States)

    Chen, Wei-Qiang; Graedel, T E

    2015-05-19

    In-use stock of a product is the amount of the product in active use. In-use product stocks provide various functions or services on which we rely in our daily work and lives, and the concept of in-use product stock for industrial ecologists is similar to the concept of net manufactured capital stock for economists. This study estimates historical physical in-use stocks of 91 products and 9 product groups and uses monetary data on net capital stocks of 56 products to either approximate or compare with in-use stocks of the corresponding products in the United States. Findings include the following: (i) The development of new products and the buildup of their in-use stocks result in the increase in variety of in-use product stocks and of manufactured capital; (ii) substitution among products providing similar or identical functions reflects the improvement in quality of in-use product stocks and of manufactured capital; and (iii) the historical evolution of stocks of the 156 products or product groups in absolute, per capita, or per-household terms shows that stocks of most products have reached or are approaching an upper limit. Because the buildup, renewal, renovation, maintenance, and operation of in-use product stocks drive the anthropogenic cycles of materials that are used to produce products and that originate from natural capital, the determination of in-use product stocks together with modeling of anthropogenic material cycles provides an analytic perspective on the material linkage between manufactured capital and natural capital.

  11. On-site methanol production plant from natural gas with modular HTR

    International Nuclear Information System (INIS)

    Takada, Eiji; Ohashi, Kazutaka; Hayakawa, Hitoshi

    1997-01-01

    The consumption of natural gas has been increasing year by year due to its relatively low level of CO 2 emissions and low cost. All of the natural gas consumed in Japan is imported from foreign countries in the form of liquid natural gas (LNG). Therefore, liquefaction, transportation and storage equipment costs are large. On the other hand, handling methanol is as easy as handling oil. If a plant for producing methanol from natural gas is sited near a natural gas field, transportation and storage costs are expected to be significantly lower than such costs for LNG production. From the above viewpoint, the concept of an on-site methanol production plant with a modular HTR (High Temperature Reactor) was studied. (author)

  12. Genome mining unveils widespread natural product biosynthetic capacity in human oral microbe Streptococcus mutans.

    Science.gov (United States)

    Liu, Liwei; Hao, Tingting; Xie, Zhoujie; Horsman, Geoff P; Chen, Yihua

    2016-11-21

    Streptococcus mutans is a major pathogen causing human dental caries. As a Gram-positive bacterium with a small genome (about 2 Mb) it is considered a poor source of natural products. Due to a recent explosion in genomic data available for S. mutans strains, we were motivated to explore the natural product production potential of this organism. Bioinformatic characterization of 169 publically available genomes of S. mutans from human dental caries revealed a surprisingly rich source of natural product biosynthetic gene clusters. Anti-SMASH analysis identified one nonribosomal peptide synthetase (NRPS) gene cluster, seven polyketide synthase (PKS) gene clusters and 136 hybrid PKS/NRPS gene clusters. In addition, 211 ribosomally synthesized and post-translationally modified peptides (RiPPs) clusters and 615 bacteriocin precursors were identified by a combined analysis using BAGEL and anti-SMASH. S. mutans harbors a rich and diverse natural product genetic capacity, which underscores the importance of probing the human microbiome and revisiting species that have traditionally been overlooked as "poor" sources of natural products.

  13. Recent advances in the elucidation of enzymatic function in natural product biosynthesis [version 2; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Gao-Yi Tan

    2016-02-01

    Full Text Available With the successful production of artemisinic acid in yeast, the promising potential of synthetic biology for natural product biosynthesis is now being realized. The recent total biosynthesis of opioids in microbes is considered to be another landmark in this field. The importance and significance of enzymes in natural product biosynthetic pathways have been re-emphasized by these advancements. Therefore, the characterization and elucidation of enzymatic function in natural product biosynthesis are undoubtedly fundamental for the development of new drugs and the heterologous biosynthesis of active natural products. Here, discoveries regarding enzymatic function in natural product biosynthesis over the past year are briefly reviewed.

  14. Recent advances in the elucidation of enzymatic function in natural product biosynthesis [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Tan Gao-Yi

    2015-12-01

    Full Text Available With the successful production of artemisinic acid in yeast, the promising potential of synthetic biology for natural product biosynthesis is now being realized. The recent total biosynthesis of opioids in microbes is considered to be another landmark in this field. The importance and significance of enzymes in natural product biosynthetic pathways have been re-emphasized by these advancements. Therefore, the characterization and elucidation of enzymatic function in natural product biosynthesis are undoubtedly fundamental for the development of new drugs and the heterologous biosynthesis of active natural products. Here, discoveries regarding enzymatic function in natural product biosynthesis over the past year are briefly reviewed.

  15. Metabolic Engineering of the Actinomycete Amycolatopsis sp. Strain ATCC 39116 towards Enhanced Production of Natural Vanillin

    OpenAIRE

    Fleige, Christian; Meyer, Florian; Steinbüchel, Alexander

    2016-01-01

    The Gram-positive bacterium Amycolatopsis sp. ATCC 39116 is used for the fermentative production of natural vanillin from ferulic acid on an industrial scale. The strain is known for its outstanding tolerance to this toxic product. In order to improve the productivity of the fermentation process, the strain's metabolism was engineered for higher final concentrations and molar yields. Degradation of vanillin could be decreased by more than 90% through deletion of the vdh gene, which codes for ...

  16. Deguelins, Natural Product Modulators of NF1-Defective Astrocytoma Cell Growth Identified by High-Throughput Screening of Partially Purified Natural Product Extracts.

    Science.gov (United States)

    Henrich, Curtis J; Cartner, Laura K; Wilson, Jennifer A; Fuller, Richard W; Rizzo, Anthony E; Reilly, Karlyne M; McMahon, James B; Gustafson, Kirk R

    2015-11-25

    A high-throughput screening assay for modulators of Trp53/NF1 mutant astrocytoma cell growth was adapted for use with natural product extracts and applied to a novel collection of prefractionated/partially purified extracts. Screening 68 427 samples identified active fractions from 95 unique extracts, including the terrestrial plant Millettia ichthyotona. Only three of these extracts showed activity in the crude extract form, thus demonstrating the utility of a partial purification approach for natural product screening. The NF1 screening assay was used to guide purification of active compounds from the M. ichthyotona extract, which yielded the two rotenones deguelin (1) and dehydrodeguelin (2). The deguelins have been reported to affect growth of a number of cancer cell lines. They potently inhibited growth of only one of a panel of NF1/Trp53 mutant murine astrocytoma cell lines, possibly related to epigenetic factors, but had no effect on the growth of normal astrocytes. These results suggest the potential utility of deguelins as tools for further investigating NF1 astrocytoma cell growth. These bioprobes were identified only as a result of screening partially purified natural product extracts.

  17. Microbial production of natural gas from coal and organic-rich shale

    Science.gov (United States)

    Orem, William

    2013-01-01

    Natural gas is an important component of the energy mix in the United States, producing greater energy yield per unit weight and less pollution compared to coal and oil. Most of the world’s natural gas resource is thermogenic, produced in the geologic environment over time by high temperature and pressure within deposits of oil, coal, and shale. About 20 percent of the natural gas resource, however, is produced by microorganisms (microbes). Microbes potentially could be used to generate economic quantities of natural gas from otherwise unexploitable coal and shale deposits, from coal and shale from which natural gas has already been recovered, and from waste material such as coal slurry. Little is known, however, about the microbial production of natural gas from coal and shale.

  18. Recreational drug discovery: natural products as lead structures for the synthesis of smart drugs.

    Science.gov (United States)

    Appendino, Giovanni; Minassi, Alberto; Taglialatela-Scafati, Orazio

    2014-07-01

    Covering: up to December 2013. Over the past decade, there has been a growing transition in recreational drugs from natural materials (marijuana, hashish, opium), natural products (morphine, cocaine), or their simple derivatives (heroin), to synthetic agents more potent than their natural prototypes, which are sometimes less harmful in the short term, or that combine properties from different classes of recreational prototypes. These agents have been named smart drugs, and have become popular both for personal consumption and for collective intoxication at rave parties. The reasons for this transition are varied, but are mainly regulatory and commercial. New analogues of known illegal intoxicants are invisible to most forensic detection techniques, while the alleged natural status and the lack of avert acute toxicity make them appealing to a wide range of users. On the other hand, the advent of the internet has made possible the quick dispersal of information among users and the on-line purchase of these agents and/or the precursors for their synthesis. Unlike their natural products chemotypes (ephedrine, mescaline, cathinone, psilocybin, THC), most new drugs of abuse are largely unfamiliar to the organic chemistry community as well as to health care providers. To raise awareness of the growing plague of smart drugs we have surveyed, in a medicinal chemistry fashion, their development from natural products leads, their current methods of production, and the role that clandestine home laboratories and underground chemists have played in the surge of popularity of these drugs.

  19. Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.

    Science.gov (United States)

    Rudolf, Jeffrey D; Chang, Chin-Yuan; Ma, Ming; Shen, Ben

    2017-08-30

    Covering: up to January 2017Cytochrome P450 enzymes (P450s) are some of the most exquisite and versatile biocatalysts found in nature. In addition to their well-known roles in steroid biosynthesis and drug metabolism in humans, P450s are key players in natural product biosynthetic pathways. Natural products, the most chemically and structurally diverse small molecules known, require an extensive collection of P450s to accept and functionalize their unique scaffolds. In this review, we survey the current catalytic landscape of P450s within the Streptomyces genus, one of the most prolific producers of natural products, and comprehensively summarize the functionally characterized P450s from Streptomyces. A sequence similarity network of >8500 P450s revealed insights into the sequence-function relationships of these oxygen-dependent metalloenzymes. Although only ∼2.4% and structurally characterized, respectively, the study of streptomycete P450s involved in the biosynthesis of natural products has revealed their diverse roles in nature, expanded their catalytic repertoire, created structural and mechanistic paradigms, and exposed their potential for biomedical and biotechnological applications. Continued study of these remarkable enzymes will undoubtedly expose their true complement of chemical and biological capabilities.

  20. Enantiomeric Mixtures in Natural Product Chemistry: Separation and Absolute Configuration Assignment.

    Science.gov (United States)

    N L Batista, Andrea; M Dos Santos, Fernando; Batista, João M; Cass, Quezia B

    2018-02-23

    Chiral natural product molecules are generally assumed to be biosynthesized in an enantiomerically pure or enriched fashion. Nevertheless, a significant amount of racemates or enantiomerically enriched mixtures has been reported from natural sources. This number is estimated to be even larger since the enantiomeric purity of secondary metabolites is rarely checked in the natural product isolation pipeline. This latter fact may have drastic effects on the evaluation of the biological activity of chiral natural products. A second bottleneck is the determination of their absolute configurations. Despite the widespread use of optical rotation and electronic circular dichroism, most of the stereochemical assignments are based on empirical correlations with similar compounds reported in the literature. As an alternative, the combination of vibrational circular dichroism and quantum chemical calculations has emerged as a powerful and reliable tool for both conformational and configurational analysis of natural products, even for those lacking UV-Vis chromophores. In this review, we aim to provide the reader with a critical overview of the occurrence of enantiomeric mixtures of secondary metabolites in nature as well the best practices for their detection, enantioselective separation using liquid chromatography, and determination of absolute configuration by means of vibrational circular dichroism and density functional theory calculations.

  1. Natural product β-thujaplicin inhibits homologous recombination repair and sensitizes cancer cells to radiation therapy.

    Science.gov (United States)

    Zhang, Lihong; Peng, Yang; Uray, Ivan P; Shen, Jianfeng; Wang, Lulu; Peng, Xiangdong; Brown, Powel H; Tu, Wei; Peng, Guang

    2017-12-01

    Investigation of natural products is an attractive strategy to identify novel compounds for cancer prevention and treatment. Numerous studies have shown the efficacy and safety of natural products, and they have been widely used as alternative treatments for a wide range of illnesses, including cancers. However, it remains unknown whether natural products affect homologous recombination (HR)-mediated DNA repair and whether these compounds can be used as sensitizers with minimal toxicity to improve patients' responses to radiation therapy, a mainstay of treatment for many human cancers. In this study, in order to systematically identify natural products with an inhibitory effect on HR repair, we developed a high-throughput image-based HR repair screening assay and screened a chemical library containing natural products. Among the most interesting of the candidate compounds identified from the screen was β-thujaplicin, a bioactive compound isolated from the heart wood of plants in the Cupressaceae family, can significantly inhibit HR repair. We further demonstrated that β-thujaplicin inhibits HR repair by reducing the recruitment of a key HR repair protein, Rad51, to DNA double-strand breaks. More importantly, our results showed that β-thujaplicin can radiosensitize cancer cells. Additionally, β-thujaplicin sensitizes cancer cells to PARP inhibitor in different cancer cell lines. Collectively, our findings for the first time identify natural compound β-thujaplicin, which has a good biosafety profile, as a novel HR repair inhibitor with great potential to be translated into clinical applications as a sensitizer to DNA-damage-inducing treatment such as radiation and PARP inhibitor. In addition, our study provides proof of the principle that our robust high-throughput functional HR repair assay can be used for a large-scale screening system to identify novel natural products that regulate DNA repair and cellular responses to DNA damage-inducing treatments such as

  2. Defining desirable natural product derived anticancer drug space: optimization of molecular physicochemical properties and ADMET attributes

    Directory of Open Access Journals (Sweden)

    Deepika Singh

    2016-06-01

    Full Text Available As part of our endeavor to enhance survival of natural product derived drug candidates and to guide the medicinal chemist to design higher probability space for success in the anti cancer drug development area, we embarked on a detailed study of the property space for a collection of natural product derived anti cancer molecules. We carried out a comprehensive analysis of properties for 24 natural products derived anti cancer drugs including clinical development candidates and a set of 27 natural products derived anti cancer lead compounds. In particular, we focused on understanding the interplay among eight physicochemical properties including like partition coefficient (log P, distribution coefficient at pH=7.4 (log D, topological polar surface area (TPSA, molecular weight (MW, aqueous solubility (log S, number of hydrogen bond acceptors (HBA, number of hydrogen bond donors (HBD and number of rotatable bonds (nRot crucial for drug design and  relationships between physicochemical properties, ADME (absorption, distribution, metabolism, and elimination attributes, and in silico toxicity profile for these two sets of compounds. This analysis provides guidance for the chemist to modify the existing natural product scaffold or designing of new anti cancer molecules in a property space with increased probability of success and may lead to the identification of druglike candidates with favorable safety profiles that can successfully test hypotheses in the clinic.

  3. Natural product modulators of transient receptor potential (TRP) channels as potential anti-cancer agents.

    Science.gov (United States)

    Rodrigues, Tiago; Sieglitz, Florian; Bernardes, Gonçalo J L

    2016-11-07

    Treatment of cancer is a significant challenge in clinical medicine, and its research is a top priority in chemical biology and drug discovery. Consequently, there is an urgent need for identifying innovative chemotypes capable of modulating unexploited drug targets. The transient receptor potential (TRPs) channels persist scarcely explored as targets, despite intervening in a plethora of pathophysiological events in numerous diseases, including cancer. Both agonists and antagonists have proven capable of evoking phenotype changes leading to either cell death or reduced cell migration. Among these, natural products entail biologically pre-validated and privileged architectures for TRP recognition. Furthermore, several natural products have significantly contributed to our current knowledge on TRP biology. In this Tutorial Review we focus on selected natural products, e.g. capsaicinoids, cannabinoids and terpenes, by highlighting challenges and opportunities in their use as starting points for designing natural product-inspired TRP channel modulators. Importantly, the de-orphanization of natural products as TRP channel ligands may leverage their exploration as viable strategy for developing anticancer therapies. Finally, we foresee that TRP channels may be explored for the selective pharmacodelivery of cytotoxic payloads to diseased tissues, providing an innovative platform in chemical biology and molecular medicine.

  4. Metabolomic application in toxicity evaluation and toxicological biomarker identification of natural product.

    Science.gov (United States)

    Chen, Dan-Qian; Chen, Hua; Chen, Lin; Tang, Dan-Dan; Miao, Hua; Zhao, Ying-Yong

    2016-05-25

    Natural product plays a vital role in disease prevention and treatment since the appearance of civilization, but the toxicity severely hinders its wide use. In order to avoid toxic effect as far as possible and use natural product safely, more comprehensive understandings of toxicity are urgently required. Since the metabolome represents the physiological or pathological status of organisms, metabolomics-based toxicology is of significance to observe potential injury before toxins have caused physiological or pathological damages. Metabolomics-based toxicology can evaluate toxicity and identify toxicological biomarker of natural product, which is helpful to guide clinical medication and reduce adverse drug reactions. In the past decades, dozens of metabolomic researches have been implemented on toxicity evaluation, toxicological biomarker identification and potential mechanism exploration of nephrotoxicity, hepatotoxicity, cardiotoxicity and central nervous system toxicity induced by pure compounds, extracts and compound prescriptions. In this paper, metabolomic technology, sample preparation, data process and analysis, and metabolomics-based toxicological research of natural product are reviewed, and finally, the potential problems and further perspectives in toxicological metabolomic investigations of natural product are discussed. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Synthesis and characterization of a small analogue of the anticancer natural product leinamycin.

    Science.gov (United States)

    Keerthi, Kripa; Rajapakse, Anuruddha; Sun, Daekyu; Gates, Kent S

    2013-01-01

    Leinamycin (1) is a Streptomyces-derived natural product that displays nanomolar IC(50) values against human cancer cell lines. In the work described here, we report the synthesis and characterization of a small leinamycin analogue 19 that closely resembles the 'upper-right quadrant' of the natural product, consisting of an alicyclic 1,2-dithiolan-3-one 1-oxide heterocycle connected to an alkene by a two-carbon linker. The results indicate that this small analogue contains the core set of functional groups required to enable thiol-triggered generation of both redox active polysulfides and an episulfonium ion intermediate via the complex reaction cascade first seen in the natural product leinamycin. The small leinamycin analogue 19 caused thiol-triggered oxidative DNA strand cleavage in a manner similar to the natural product, but did not alkyate duplex DNA effectively. This highlights the central role of the 18-membered macrocycle of leinamycin in driving efficient DNA alkylation by the natural product. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Virtual screening on natural products for discovering active compounds and target information.

    Science.gov (United States)

    Shen, Jianhua; Xu, Xiaoying; Cheng, Feng; Liu, Hong; Luo, Xiaomin; Shen, Jingkang; Chen, Kaixian; Zhao, Weimin; Shen, Xu; Jiang, Hualiang

    2003-11-01

    Natural products, containing inherently large-scale structural diversity than synthetic compounds, have been the major resources of bioactive agents and will continually play as protagonists for discovering new drugs. However, how to access this diverse chemical space efficiently and effectively is an exciting challenge for medicinal chemists and pharmacologists. While virtual screening, which has shown a great promise in drug discovery, will play an important role in digging out lead (active) compounds from natural products. This review focuses on the strategy of virtual screening based on molecular docking and, with successful examples from our laboratory, illustrates the efficiency of virtual screening in discovering active compounds from natural products. On the other hand, the sequencing of the human genome and numerous pathogen genomes has resulted in an unprecedented opportunity for discovering potential new drug targets. Chemogenomics has appeared as a new technology to initiate target discovery by using active compounds as probes to characterize proteome functions. Natural products are the ideal probes for such research. Binding affinity fingerprint is a powerful chemogenomic descriptor to characterize both small molecules and pharmacologically relevant proteins. Therefore, this review also discusses binding affinity fingerprint strategy for identifying target information from the genomic data by using natural products as the probes.

  7. Neutral loss fragmentation pattern based screening for arginine-rich natural products in Xenorhabdus and Photorhabdus.

    Science.gov (United States)

    Fuchs, Sebastian W; Sachs, Christian C; Kegler, Carsten; Nollmann, Friederike I; Karas, Michael; Bode, Helge B

    2012-08-21

    Although sharing a certain degree of structural uniformity, natural product classes exhibit variable functionalities such as different amino acid or acyl residues. During collision induced dissociation, some natural products exhibit a conserved fragmentation pattern close to the precursor ion. The observed fragments result from a shared set of neutral losses, creating a unique fragmentation pattern, which can be used as a fingerprint for members of these natural product classes. The culture supernatants of 69 strains of the entomopathogenic bacteria Photorhabdus and Xenorhabdus were analyzed by MALDI-MS(2), and a database comprising MS(2) data from each strain was established. This database was scanned for concordant fragmentation patterns of different compounds using a customized software, focusing on relative mass differences of the fragment ions to their precursor ion. A novel group of related natural products comprising 25 different arginine-rich peptides from 16 different strains was identified due to its characteristic neutral loss fragmentation pattern, and the structures of eight compounds were elucidated. Two biosynthesis gene clusters encoding nonribosomal peptide synthetases were identified, emphasizing the possibility to identify a group of structurally and biosynthetically related natural products based on their neutral loss fragmentation pattern.

  8. Natural Resources and Local Development: The Argentinian Oilseed Complex and Biofuel Production

    Directory of Open Access Journals (Sweden)

    Eliana Scialabba

    2014-01-01

    Full Text Available Argentina is very rich in natural resources, particularly those linked to the oilseed production. Global demand and improved means of production, have resulted in a mature and developed production infrastructure geared toward export. This generates not just revenue, but also has other positive effects, such as the creation of jobs and sustainable development. In this context, biofuel production adds multidimensional value to the vegetable oil industry and generations many spin-off industries.

  9. Medicinal plants and natural products in amelioration of arsenic toxicity: a short review.

    Science.gov (United States)

    Bhattacharya, Sanjib

    2017-12-01

    Chronic arsenic toxicity (arsenicosis) is considered a serious public health menace worldwide, as there is no specific, safe, and efficacious therapeutic management of arsenicosis. To collate the studies on medicinal plants and natural products with arsenic toxicity ameliorative effect, active pre-clinically and/or clinically. Literature survey was carried out by using Google, Scholar Google and Pub-Med. Only the scientific journal articles found on the internet for last two decades were considered. Minerals and semi-synthetic or synthetic analogs of natural products were excluded. Literature study revealed that 34 medicinal plants and 14 natural products exhibited significant protection from arsenic toxicity, mostly in preclinical trials and a few in clinical studies. This research could lead to development of a potentially useful agent in clinical management of arsenicosis in humans.

  10. Brazilian Propolis: A Natural Product That Improved the Fungicidal Activity by Blood Phagocytes

    Science.gov (United States)

    Possamai, Muryllo Mendes; Honorio-França, Adenilda Cristina; Reinaque, Ana Paula Barcelos; França, Eduardo Luzia; Souto, Paula Cristina de Souza

    2013-01-01

    Natural product incorporation into microcarriers increases the bioavailability of these compounds, consequently improving their therapeutic properties. Natural products, particularly those from bees such as propolis, are widely used in popular medicine. Propolis is a powerful treatment for several diseases. In this context, the present study evaluated the effect of propolis Scaptotrigona sp. and its fractions, alone or adsorbed to polyethylene glycol (PEG) microspheres, on the activity of human phagocytes against Candida albicans. The results show that propolis exerts a stimulatory effect on these cells to assist in combating the fungus, especially as the crude extract is compared with the fractions. However, when incorporated into microspheres, these properties were significantly potentiated. These results suggest that propolis adsorbed onto PEG microspheres has immunostimulatory effects on phagocytes in human blood. Therefore, propolis may potentially be an additional natural product that can be used for a variety of therapies. PMID:23509737

  11. An assessment of natural product discovery from marine (sensu strictu) and marine-derived fungi

    Science.gov (United States)

    Overy, David P.; Bayman, Paul; Kerr, Russell G.; Bills, Gerald F.

    2014-01-01

    The natural products community has been investigating secondary metabolites from marine fungi for several decades, but when one attempts to search for validated reports of new natural products from marine fungi, one encounters a literature saturated with reports from ‘marine-derived’ fungi. Of the 1000+ metabolites that have been characterized to date, only approximately 80 of these have been isolated from species from exclusively marine lineages. These metabolites are summarized here along with the lifestyle and habitats of their producing organisms. Furthermore, we address some of the reasons for the apparent disconnect between the stated objectives of discovering new chemistry from marine organisms and the apparent neglect of the truly exceptional obligate marine fungi. We also offer suggestions on how to reinvigorate enthusiasm for marine natural products discovery from fungi from exclusive marine lineages and highlight the need for critically assessing the role of apparently terrestrial fungi in the marine environment. PMID:25379338

  12. Natural Product Inspired N-Terminal Hsp90 Inhibitors: From Bench to Bedside?

    Science.gov (United States)

    Khandelwal, Anuj; Crowley, Vincent M; Blagg, Brian S J

    2016-01-01

    The 90 kDa heat shock proteins (Hsp90) are responsible for the conformational maturation of nascent polypeptides and the rematuration of denatured proteins. Proteins dependent upon Hsp90 are associated with all six hallmarks of cancer. Upon Hsp90 inhibition, protein substrates are degraded via the ubiquitin-proteasome pathway. Consequentially, inhibition of Hsp90 offers a therapeutic opportunity for the treatment of cancer. Natural product inhibitors of Hsp90 have been identified in vitro, which have served as leads for the development of more efficacious inhibitors and analogs that have entered clinical trials. This review highlights the development of natural product analogs, as well as the development of clinically important inhibitors that arose from natural products. © 2015 Wiley Periodicals, Inc.

  13. Crystalline-Sponge-Based Structural Analysis of Crude Natural Product Extracts.

    Science.gov (United States)

    Wada, Naoki; Kersten, Roland D; Iwai, Takahiro; Lee, Shoukou; Sakurai, Fumie; Kikuchi, Takashi; Fujita, Daishi; Fujita, Makoto; Weng, Jing-Ke

    2018-03-26

    Characterization of complex natural product mixtures to the absolute structural level of their components often requires significant amounts of starting materials and lengthy purification process, followed by arduous structure elucidation efforts. The crystalline sponge (CS) method has demonstrated utility in the absolute structure elucidation of isolated organic compounds at miniscule quantities compared to conventional methods. In this work, we developed a new CS-based workflow that greatly expedites the in-depth structural analysis of crude natural product extracts. Using a crude extract of the red alga Laurencia pacifica, we showed that CS affinity screening prior to compound isolation enables prioritization of analytes present in the extract, and we subsequently resolved the molecular structures of six sesquiterpenes with stereochemical clarity from around 10 mg crude extract. This study demonstrates a new chemotyping workflow that can greatly accelerate natural product discovery from complex samples. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Synthesis and Neurotrophic Activity Studies of Illicium Sesquiterpene Natural Product Analogues.

    Science.gov (United States)

    Richers, Johannes; Pöthig, Alexander; Herdtweck, Eberhardt; Sippel, Claudia; Hausch, Felix; Tiefenbacher, Konrad

    2017-03-02

    Neurotrophic natural products hold potential as privileged structures for the development of therapeutic agents against neurodegeneration. However, only a few studies have been conducted to investigate a common pharmacophoric motif and structure-activity relationships (SARs). Here, an investigation of structurally more simple analogues of neurotrophic sesquiterpenes of the illicium family is presented. A concise synthetic route enables preparation of the carbon framework of (±)-Merrilactone A and (±)-Anislactone A/B on a gram scale. This has allowed access to a series of structural analogues by modification of the core structure, including variation of oxidation levels and alteration of functional groups. In total, 15 derivatives of the natural products have been synthesized and tested for their neurite outgrowth activities. Our studies indicate that the promising biological activity can be retained by structurally simpler natural product analogues, which are accessible by a straightforward synthetic route. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Natural antifouling compound production by microbes associated with marine macroorganisms — A review

    Directory of Open Access Journals (Sweden)

    Sathianeson Satheesh

    2016-05-01

    Full Text Available In the marine environment, all hard surfaces including marine macroorganims are colonized by microorganisms mainly from the surrounding environment. The microorganisms associated with marine macroorganisms offer tremendous potential for exploitation of bioactive metabolites. Biofouling is a continuous problem in marine sectors which needs huge economy for control and cleaning processes. Biotechnological way for searching natural product antifouling compounds gained momentum in recent years because of the environmental pollution associated with the use of toxic chemicals to control biofouling. While, natural product based antifoulants from marine organisms particularly sponges and corals attained significance due to their activities in field assays, collection of larger amount of organisms from the sea is not a viable one. The microorganisms associated with sponges, corals, ascidians, seaweeds and seagrasses showed strong antimicrobial and also antifouling activities. This review highlights the advances in natural product antifoulants research from microbes associated with marine organisms.

  16. Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics

    Directory of Open Access Journals (Sweden)

    Andrew M. Piggott

    2005-06-01

    Full Text Available Reverse chemical proteomics combines affinity chromatography with phage display and promises to be a powerful new platform technology for the isolation of natural product receptors, facilitating the drug discovery process by rapidly linking biologically active small molecules to their cellular receptors and the receptors’ genes. In this paper we review chemical proteomics and reverse chemical proteomics and show how these techniques can add value to natural products research. We also report on techniques for the derivatisation of polystyrene microtitre plates with cleavable linkers and marine natural products that can be used in chemical proteomics or reverse chemical proteomics. Specifically, we have derivatised polystyrene with palau’amine and used reverse chemical proteomics to try and isolate the human receptors for this potent anticancer marine drug.

  17. Exploring anti-TB leads from natural products library originated from marine microbes and medicinal plants.

    Science.gov (United States)

    Liu, Xueting; Chen, Caixia; He, Wenni; Huang, Pei; Liu, Miaomiao; Wang, Qian; Guo, Hui; Bolla, Krishna; Lu, Yan; Song, Fuhang; Dai, Huanqin; Liu, Mei; Zhang, Lixin

    2012-10-01

    Multidrug-resistant tuberculosis (MDR-TB) and TB-HIV co-infection have become a great threat to global health. However, the last truly novel drug that was approved for the treatment of TB was discovered 40 years ago. The search for new effective drugs against TB has never been more intensive. Natural products derived from microbes and medicinal plants have been an important source of TB therapeutics. Recent advances have been made to accelerate the discovery rate of novel TB drugs including diversifying strategies for environmental strains, high-throughput screening (HTS) assays, and chemical diversity. This review will discuss the challenges of finding novel natural products with anti-TB activity from marine microbes and plant medicines, including biodiversity- and taxonomy-guided microbial natural products library construction, target- and cell-based HTS, and bioassay-directed isolation of anti-TB substances from traditional medicines.

  18. Research Trends : Natural Products as Pest, Microbial Disease an Tumour Control Agents

    Directory of Open Access Journals (Sweden)

    M.O. Fatope

    2000-12-01

    Full Text Available The zeal to conquer human, agronomic, and veterinary diseases or pests has always sustained research interests directed at finding new medications from under explored biodiversity. The adoption of bioassay methods, which correlate with prevention, inhibition and reversal of diseases or elimination of pests, in screening natural products for bioactive compounds is critical to the discovery of new medications. This article focuses on areas of natural products chemistry that connect academia with industry. It discusses the technical skills needed to find bioactive compounds from microbes, marine invertebrates, plants and insects, highlighting the mode of action of selected bioactive compounds and bioassay models for detecting them. It also reviews methods and prospects of genetic manipulation of organisms to produce unnatural natural products of therapeutic importance.

  19. Effects of wood vinegar as an additive for natural rubber products

    Directory of Open Access Journals (Sweden)

    Kulchanat Prasertsit

    2011-08-01

    Full Text Available Wood vinegar, a product of wood burning in airless condition, is well known as an all- purpose natural chemical, since it can beused in agricultural, food, and pharmaceutical industries, even for home care products. Because of its acidity and anti microorganismgrowth property, it is of interest to use wood vinegar for rubber products by fully or partially replacing the usage oftraditional acids in the production processes. This work is to study effects of crude coconut shell wood vinegar on naturalrubber products. The experiment was conducted by introducing various concentrations of wood vinegar to block and sheetrubbers. Results showed that wood vinegar can improve initial plasticity, plasticity retention index and Mooney viscosity ofrubber products. Moreover, it can also retard the growth of fungi in the products.

  20. Discovery and resupply of pharmacologically active plant-derived natural products: A review

    Science.gov (United States)

    Linder, Thomas; Wawrosch, Christoph; Uhrin, Pavel; Temml, Veronika; Wang, Limei; Schwaiger, Stefan; Heiss, Elke H.; Rollinger, Judith M.; Schuster, Daniela; Breuss, Johannes M.; Bochkov, Valery; Mihovilovic, Marko D.; Kopp, Brigitte; Bauer, Rudolf; Dirsch, Verena M.; Stuppner, Hermann

    2016-01-01

    Medicinal plants have historically proven their value as a source of molecules with therapeutic potential, and nowadays still represent an important pool for the identification of novel drug leads. In the past decades, pharmaceutical industry focused mainly on libraries of synthetic compounds as drug discovery source. They are comparably easy to produce and resupply, and demonstrate good compatibility with established high throughput screening (HTS) platforms. However, at the same time there has been a declining trend in the number of new drugs reaching the market, raising renewed scientific interest in drug discovery from natural sources, despite of its known challenges. In this survey, a brief outline of historical development is provided together with a comprehensive overview of used approaches and recent developments relevant to plant-derived natural product drug discovery. Associated challenges and major strengths of natural product-based drug discovery are critically discussed. A snapshot of the advanced plant-derived natural products that are currently in actively recruiting clinical trials is also presented. Importantly, the transition of a natural compound from a “screening hit” through a “drug lead” to a “marketed drug” is associated with increasingly challenging demands for compound amount, which often cannot be met by re-isolation from the respective plant sources. In this regard, existing alternatives for resupply are also discussed, including different biotechnology approaches and total organic synthesis. While the intrinsic complexity of natural product-based drug discovery necessitates highly integrated interdisciplinary approaches, the reviewed scientific developments, recent technological advances, and research trends clearly indicate that natural products will be among the most important sources of new drugs also in the future. PMID:26281720

  1. Natural gas productive capacity for the lower 48 states 1984 through 1996, February 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-09

    This is the fourth wellhead productive capacity report. The three previous ones were published in 1991, 1993, and 1994. This report should be of particular interest to those in Congress, Federal and State agencies, industry, and the academic community, who are concerned with the future availability of natural gas. The EIA Dallas Field Office has prepared five earlier reports regarding natural gas productive capacity. These reports, Gas Deliverability and Flow Capacity of Surveillance Fields, reported deliverability and capacity data for selected gas fields in major gas producing areas. The data in the reports were based on gas-well back-pressure tests and estimates of gas-in-place for each field or reservoir. These reports use proven well testing theory, most of which has been employed by industry since 1936 when the Bureau of Mines first published Monograph 7. Demand for natural gas in the United States is met by a combination of natural gas production, underground gas storage, imported gas, and supplemental gaseous fuels. Natural gas production requirements in the lower 48 States have been increasing during the last few years while drilling has remained at low levels. This has raised some concern about the adequacy of future gas supplies, especially in periods of peak heating or cooling demand. The purpose of this report is to address these concerns by presenting a 3-year projection of the total productive capacity of natural gas at the wellhead for the lower 48 States. Alaska is excluded because Alaskan gas does not enter the lower-48 States pipeline system. The Energy Information Administration (EIA) generates this 3-year projection based on historical gas-well drilling and production data from State, Federal, and private sources. In addition to conventional gas-well gas, coalbed gas and oil-well gas are also included.

  2. Natural gas productive capacity for the lower 48 states 1984 through 1996, February 1996

    International Nuclear Information System (INIS)

    1996-01-01

    This is the fourth wellhead productive capacity report. The three previous ones were published in 1991, 1993, and 1994. This report should be of particular interest to those in Congress, Federal and State agencies, industry, and the academic community, who are concerned with the future availability of natural gas. The EIA Dallas Field Office has prepared five earlier reports regarding natural gas productive capacity. These reports, Gas Deliverability and Flow Capacity of Surveillance Fields, reported deliverability and capacity data for selected gas fields in major gas producing areas. The data in the reports were based on gas-well back-pressure tests and estimates of gas-in-place for each field or reservoir. These reports use proven well testing theory, most of which has been employed by industry since 1936 when the Bureau of Mines first published Monograph 7. Demand for natural gas in the United States is met by a combination of natural gas production, underground gas storage, imported gas, and supplemental gaseous fuels. Natural gas production requirements in the lower 48 States have been increasing during the last few years while drilling has remained at low levels. This has raised some concern about the adequacy of future gas supplies, especially in periods of peak heating or cooling demand. The purpose of this report is to address these concerns by presenting a 3-year projection of the total productive capacity of natural gas at the wellhead for the lower 48 States. Alaska is excluded because Alaskan gas does not enter the lower-48 States pipeline system. The Energy Information Administration (EIA) generates this 3-year projection based on historical gas-well drilling and production data from State, Federal, and private sources. In addition to conventional gas-well gas, coalbed gas and oil-well gas are also included

  3. Evaluation of culture media for the production of secondary metabolites in a natural products screening program.

    Science.gov (United States)

    Vandermolen, Karen M; Raja, Huzefa A; El-Elimat, Tamam; Oberlies, Nicholas H

    2013-12-17

    Variation in the growing environment can have significant impacts on the quantity and diversity of fungal secondary metabolites. In the industrial setting, optimization of growing conditions can lead to significantly increased production of a compound of interest. Such optimization becomes challenging in a drug-discovery screening situation, as the ideal conditions for one organism may induce poor metabolic diversity for a different organism. Here, the impact of different media types, including six liquid media and five solid media, on the secondary metabolite production of three fungal strains was examined in the context of the drug-discovery screening process. The relative production of marker compounds was used to evaluate the usefulness and reliability of each medium for the purpose of producing secondary metabolites.

  4. Natural gas productive capacity for the lower 48 States, 1980 through 1995

    International Nuclear Information System (INIS)

    1994-01-01

    The purpose of this report is to analyze monthly natural gas wellhead productive capacity in the lower 48 States from 1980 through 1992 and project this capacity from 1993 through 1995. For decades, natural gas supplies and productive capacity have been adequate to meet demand. In the 1970's the capacity surplus was small because of market structure (split between interstate and intrastate), increasing demand, and insufficient drilling. In the early 1980's, lower demand, together with increased drilling, led to a large surplus capacity as new productive capacity came on line. After 1986, this large surplus began to decline as demand for gas increased, gas prices fell, and gas well completions dropped sharply. In late December 1989, the decline in this surplus, accompanied by exceptionally high demand and temporary weather-related production losses, led to concerns about the adequacy of monthly productive capacity for natural gas. These concerns should have been moderated by the gas system's performance during the unusually severe winter weather in March 1993 and January 1994. The declining trend in wellhead productive capacity is expected to be reversed in 1994 if natural gas prices and drilling meet or exceed the base case assumption. This study indicates that in the low, base, and high drilling cases, monthly productive capacity should be able to meet normal production demands through 1995 in the lower 48 States (Figure ES1). Exceptionally high peak-day or peak-week production demand might not be met because of physical limitations such as pipeline capacity. Beyond 1995, as the capacity of currently producing wells declines, a sufficient number of wells and/or imports must be added each year in order to ensure an adequate gas supply

  5. Accuracy, Precision, and Reliability of Chemical Measurements in Natural Products Research

    Science.gov (United States)

    Betz, Joseph M.; Brown, Paula N.; Roman, Mark C.

    2010-01-01

    Natural products chemistry is the discipline that lies at the heart of modern pharmacognosy. The field encompasses qualitative and quantitative analytical tools that range from spectroscopy and spectrometry to chromatography. Among other things, modern research on crude botanicals is engaged in the discovery of the phytochemical constituents necessary for therapeutic efficacy, including the synergistic effects of components of complex mixtures in the botanical matrix. In the phytomedicine field, these botanicals and their contained mixtures are considered the active pharmaceutical ingredient (API), and pharmacognosists are increasingly called upon to supplement their molecular discovery work by assisting in the development and utilization of analytical tools for assessing the quality and safety of these products. Unlike single-chemical entity APIs, botanical raw materials and their derived products are highly variable because their chemistry and morphology depend on the genotypic and phenotypic variation, geographical origin and weather exposure, harvesting practices, and processing conditions of the source material. Unless controlled, this inherent variability in the raw material stream can result in inconsistent finished products that are under-potent, over-potent, and/or contaminated. Over the decades, natural products chemists have routinely developed quantitative analytical methods for phytochemicals of interest. Quantitative methods for the determination of product quality bear the weight of regulatory scrutiny. These methods must be accurate, precise, and reproducible. Accordingly, this review discusses the principles of accuracy (relationship between experimental and true value), precision (distribution of data values), and reliability in the quantitation of phytochemicals in natural products. PMID:20884340

  6. Natural Fostering in Fritillaria cirrhosa: Integrating herbal medicine production with biodiversity conservation

    Directory of Open Access Journals (Sweden)

    Xiwen Li

    2012-02-01

    Full Text Available Protected areas are generally regarded as a power tool to conserve biodiversity. Nonetheless, few protected areas could address three crucial problems simultaneously, namely funding, public participation and rural living. Here, we introduced a new protective approach, Natural Fostering, which integrated herbal medicine production with community conservation. The principles of Natural Fostering adopted species–species interaction at community level. Most effective chemical components of herbal medicine are derived from such interaction. Fritillaria cirrhosa was selected as an economic botany, one of herbal medicines, to carry out Natural Fostering. Community habitats, herbal medicine production, funding and income of local family were investigated to verify the feasibility of Natural Fostering for biodiversity. We found the density of plant populations and the annual average personal income of rural people increased. F. cirrhosa production could provide sufficient funds for sustainable conservation. Local people gradually changed their life style of wild collection and overgrazing, instead of herbal medicine production. The fostering area set up a good sustainable economic cycle. Natural Fostering can be presented as an effective and pragmatic way to conserve biological diversity and sustainable utilization of traditional medicinal resources.

  7. An overview of the marine natural products in clinical trials and on the market

    Directory of Open Access Journals (Sweden)

    Marisa Rangel

    2015-06-01

    Full Text Available The first marine natural products that served as leads or scaffolds for medicines were discovered in the middle of last century: the arabinosyl glycosides from the marine sponge Tectitethya crypta. Synthesis and modifications of the natural molecules generated antiviral and antileukemic drugs developed in the 1970’s and in the following decades, including the first effective treatment against HIV infection. With the improvement of techniques for the elucidation of chemical structure of the molecules, as well as chemical synthesis, especially from the 1990’s, there was an increase in the number of bioactive natural products characterized from marine organisms. New chemical structures with high specificity towards molecular targets in cells allowed the development of new drugs with indication for the treatment of several illnesses, from cancer to new antibiotics, and even neurological disorders. Currently there are at least 13 molecules derived from marine natural products on advanced clinical trials, and nine were approved to be used as medicines. Considering that in the past eight years, more than 1000 new compounds from marine organisms were described, per year, the expectation is that many more drugs will be derived from marine natural products in a near future.

  8. Forecasting natural gas supply in China: Production peak and import trends

    International Nuclear Information System (INIS)

    Lin Boqiang; Wang Ting

    2012-01-01

    China's natural gas consumption has increased rapidly in recent years making China a net gas importer. As a nonrenewable energy, the gas resource is exhaustible. Based on the forecast of this article, China's gas production peak is likely to approach in 2022. However, China is currently in the industrialization and urbanization stage, and its natural gas consumption will persistently increase. With China's gas production peak, China will have to face a massive expansion in gas imports. As the largest developing country, China's massive imports of gas will have an effect on the international gas market. In addition, as China's natural gas price is still controlled by the government and has remained at a low level, the massive imports of higher priced gas will exert great pressure on China's gas price reform. - Highlights: ► We figured out the natural gas production peak of China. ► We predict the import trends of natural gas of China. ► We study the international and national impacts of China's increasing import of gas. ► It is important for China to accelerate price reformation of natural gas.

  9. Expanding the range of 'druggable' targets with natural product-based libraries: an academic perspective.

    Science.gov (United States)

    Bauer, Renato A; Wurst, Jacqueline M; Tan, Derek S

    2010-06-01

    Existing drugs address a relatively narrow range of biological targets. As a result, libraries of drug-like molecules have proven ineffective against a variety of challenging targets, such as protein-protein interactions, nucleic acid complexes, and antibacterial modalities. In contrast, natural products are known to be effective at modulating such targets, and new libraries are being developed based on underrepresented scaffolds and regions of chemical space associated with natural products. This has led to several recent successes in identifying new chemical probes that address these challenging targets. Copyright 2010 Elsevier Ltd. All rights reserved.

  10. Marine sponge derived natural products between 2001 and 2010: trends and opportunities for discovery of bioactives.

    Science.gov (United States)

    Mehbub, Mohammad Ferdous; Lei, Jie; Franco, Christopher; Zhang, Wei

    2014-08-19

    Marine sponges belonging to the phylum Porifera (Metazoa), evolutionarily the oldest animals are the single best source of marine natural products. The present review presents a comprehensive overview of the source, taxonomy, country of origin or geographical position, chemical class, and biological activity of sponge-derived new natural products discovered between 2001 and 2010. The data has been analyzed with a view to gaining an outlook on the future trends and opportunities in the search for new compounds and their sources from marine sponges.

  11. Desymmetrization of 7-azabicycloalkenes by tandem olefin metathesis for the preparation of natural product scaffolds

    Directory of Open Access Journals (Sweden)

    Deppermann Nina

    2007-12-01

    Full Text Available Abstract Background Tandem olefin metathesis sequences are known to be versatile for the generation of natural product scaffolds and have also been used for ring opening of strained carbo- and heterocycles. In this paper we demonstrate the potential of these reactions for the desymmetrization of 7-azabicycloalkenes. Results We have established efficient protocols for the desymmetrization of different 7-azabicycloalkenes by intra- and intermolecular tandem metathesis sequences with ruthenium based catalysts. Conclusion Desymmetrization of 7-azabicycloalkenes by olefin metathesis is an efficient process for the preparation of common natural product scaffolds such as pyrrolidines, indolizidines and isoindoles.

  12. Desymmetrization of 7-azabicycloalkenes by tandem olefin metathesis for the preparation of natural product scaffolds

    Science.gov (United States)

    Maison, Wolfgang; Büchert, Marina; Deppermann, Nina

    2007-01-01

    Background Tandem olefin metathesis sequences are known to be versatile for the generation of natural product scaffolds and have also been used for ring opening of strained carbo- and heterocycles. In this paper we demonstrate the potential of these reactions for the desymmetrization of 7-azabicycloalkenes. Results We have established efficient protocols for the desymmetrization of different 7-azabicycloalkenes by intra- and intermolecular tandem metathesis sequences with ruthenium based catalysts. Conclusion Desymmetrization of 7-azabicycloalkenes by olefin metathesis is an efficient process for the preparation of common natural product scaffolds such as pyrrolidines, indolizidines and isoindoles. PMID:18088413

  13. Structural aspects of phenylglycines, their biosynthesis and occurrence in peptide natural products.

    Science.gov (United States)

    Al Toma, Rashed S; Brieke, Clara; Cryle, Max J; Süssmuth, Roderich D

    2015-08-01

    Phenylglycine-type amino acids occur in a wide variety of peptide natural products, including glycopeptide antibiotics and biologically active linear and cyclic peptides. Sequencing of biosynthesis gene clusters of chloroeremomycin, balhimycin and pristinamycin paved the way for intensive investigations on the biosynthesis of 4-hydroxyphenylglycine (Hpg), 3,5-dihydroxyphenylglycine (Dpg) and phenylglycine (Phg) in recent years. The significance and importance of this type of unusual non-proteinogenic aromatic amino acids also for medicinal chemistry has drawn the attention of many research groups and pharmaceutical companies. Herein structures and properties of phenylglycine containing natural products as well as the biosynthetic origin and incorporation of phenylglycines are discussed.

  14. Structure of a putative fluorinated natural product from Streptomyces sp. TC1.

    Science.gov (United States)

    Aldemir, Hülya; Kohlhepp, Stefanie V; Gulder, Tanja; Gulder, Tobias A M

    2014-11-26

    Fluorine-containing natural products are extremely rare. The recent report on the isolation and biological activity of the bacterial secondary metabolite 3-(3,5-di-tert-butyl-4-fluorophenyl)propionic acid was thus highly remarkable. The compound contained the first aromatic fluorine substituent known to date in any natural product. The promise to discover an enzyme capable of aromatic fluorination in the producing strain Streptomyces sp. TC1 prompted our immediate interest. A close inspection of the originally reported analytical data of the fluoro metabolite revealed inconsistencies that triggered us to validate the reported structure. The results of these efforts are presented in this communication.

  15. Investigating the Structure-Activity Relationship of the Insecticidal Natural Product Rocaglamide.

    Science.gov (United States)

    Hall, Roger G; Bruce, Ian; Cooke, Nigel G; Diorazio, Louis J; Cederbaum, Fredrik; Dobler, Markus R; Irving, Ed

    2017-12-01

    The natural product Rocaglamide (1), isolated from the tree Aglaia elliptifolia, is a compelling but also challenging lead structure for crop protection. In laboratory assays, the natural product shows highly interesting insecticidal activity against chewing pests and beetles, but also phytotoxicity on some crop plants. Multi-step syntheses with control of stereochemistry were required to probe the structure-activity relationship (SAR), and seek simplified analogues. After a significant research effort, just two areas of the molecule were identified which allow modification whilst maintaining activity, as will be highlighted in this paper.

  16. The importance of asking "how and why?" in natural product structure elucidation.

    Science.gov (United States)

    Brown, Patrick D; Lawrence, Andrew L

    2017-10-18

    Covering: up to 2017This review highlights why careful consideration of the biosynthetic origin (the how) and the biological function (the why) of a natural product can be so useful during the determination of its structure. Recent examples of structural reassignments inspired by biosynthetic and functional insights will be presented. This review will demonstrate the importance of viewing the origin, structure and function of a natural product as intertwined threads of a single story, best viewed as a whole rather than as discrete topics.

  17. Gluten Contamination in Naturally or Labeled Gluten-Free Products Marketed in Italy

    Science.gov (United States)

    Verma, Anil K.; Gatti, Simona; Galeazzi, Tiziana; Monachesi, Chiara; Padella, Lucia; Baldo, Giada Del; Annibali, Roberta; Lionetti, Elena; Catassi, Carlo

    2017-01-01

    Background: A strict and lifelong gluten-free diet is the only treatment of celiac disease. Gluten contamination has been frequently reported in nominally gluten-free products. The aim of this study was to test the level of gluten contamination in gluten-free products currently available in the Italian market. Method: A total of 200 commercially available gluten-free products (including both naturally and certified gluten-free products) were randomly collected from different Italian supermarkets. The gluten content was determined by the R5 ELISA Kit approved by EU regulations. Results: Gluten level was lower than 10 part per million (ppm) in 173 products (86.5%), between 10 and 20 ppm in 9 (4.5%), and higher than 20 ppm in 18 (9%), respectively. In contaminated foodstuff (gluten > 20 ppm) the amount of gluten was almost exclusively in the range of a very low gluten content. Contaminated products most commonly belonged to oats-, buckwheat-, and lentils-based items. Certified and higher cost gluten-free products were less commonly contaminated by gluten. Conclusion: Gluten contamination in either naturally or labeled gluten-free products marketed in Italy is nowadays uncommon and usually mild on a quantitative basis. A program of systematic sampling of gluten-free food is needed to promptly disclose at-risk products. PMID:28178205

  18. Factors affecting industrial wood, material production yield in Turkey’s natural beech forests

    Directory of Open Access Journals (Sweden)

    Atilla Atik

    2014-07-01

    Full Text Available The objectives of the present study are to determine the most important factors affecting industrial wood material production yield in natural oriental beech forests in Turkey using a multifaceted approach and to help entrepreneurs consider these factors to develop more sensitive and realistic production plans. In Günye Forest Management in Bartın province of the West Black Sea Region of Turkey, 41 production units were chosen as the study area. The 1277 ha study area was included in the 2007 and 2010 production management plan. The general state of the stand, natural stand structure, and production methods and tools are the factors thought most strongly affect industrial wood material production yield; 26 variables representing these factors were evaluated in the study. Through multidimensional statistical analyses, including main components, factor and regression  analysis, we found that the most important factors affecting production yield were fertility, aspect of land, skidding method, stand structure, skidding distance, growing stock, transportation and harmful abiotic factors. Production units were divided into three groups based on yield rates and the 26 variables, using discriminate analysis. From the results of the study, a sample model can be developed to help forest managers predict and plan annual industrial wood production more sensitively and realistically.

  19. Computational strategies for genome-based natural product discovery and engineering in fungi.

    Science.gov (United States)

    van der Lee, Theo A J; Medema, Marnix H

    2016-04-01

    Fungal natural products possess biological activities that are of great value to medicine, agriculture and manufacturing. Recent metagenomic studies accentuate the vastness of fungal taxonomic diversity, and the accompanying specialized metabolic diversity offers a great and still largely untapped resource for natural product discovery. Although fungal natural products show an impressive variation in chemical structures and biological activities, their biosynthetic pathways share a number of key characteristics. First, genes encoding successive steps of a biosynthetic pathway tend to be located adjacently on the chromosome in biosynthetic gene clusters (BGCs). Second, these BGCs are often are located on specific regions of the genome and show a discontinuous distribution among evolutionarily related species and isolates. Third, the same enzyme (super)families are often involved in the production of widely different compounds. Fourth, genes that function in the same pathway are often co-regulated, and therefore co-expressed across various growth conditions. In this mini-review, we describe how these partly interlinked characteristics can be exploited to computationally identify BGCs in fungal genomes and to connect them to their products. Particular attention will be given to novel algorithms to identify unusual classes of BGCs, as well as integrative pan-genomic approaches that use a combination of genomic and metabolomic data for parallelized natural product discovery across multiple strains. Such novel technologies will not only expedite the natural product discovery process, but will also allow the assembly of a high-quality toolbox for the re-design or even de novo design of biosynthetic pathways using synthetic biology approaches. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  20. Natural Products at Work: Structural Insights into Inhibition of the Bacterial Membrane Protein MraY.

    Science.gov (United States)

    Koppermann, Stefan; Ducho, Christian

    2016-09-19

    Natural(ly) fit: The X-ray crystal structure of the bacterial membrane protein MraY in complex with its natural product inhibitor muraymycin D2 is discussed. MraY catalyzes one of the membrane-associated steps in peptidoglycan biosynthesis and, therefore, represents a promising target for novel antibiotics. Structural insights derived from the protein-inhibitor complex might now pave the way for the development of new antimicrobial drugs. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. How does increased corn-ethanol production affect US natural gas prices?

    International Nuclear Information System (INIS)

    Whistance, Jarrett; Thompson, Wyatt

    2010-01-01

    In recent years, there has been a push to increase biofuel production in the United States. The biofuel of choice, so far, has been ethanol produced from corn. The effects of increased corn-ethanol production on the consumer prices of food and energy continue to be studied and debated. This study examines, in particular, the effects of increased corn-ethanol production on US natural gas prices. A structural model of the natural gas market is developed and estimated using two stage least squares. A baseline projection for the period 2007-2018 is determined, and two scenarios are simulated. In the first scenario, current biofuel policies including EISA mandates, tariffs, and tax credits are removed. In the second scenario, we hold ethanol production to the level required only for largely obligatory additive use. The results indicate that the increased level of corn-ethanol production occurring as a result of the current US biofuel policies may lead to natural gas prices that are as much as 0.25% higher, on average, than if no biofuel policies were in place. A similar comparison between the baseline and second scenario indicates natural gas prices could be as much as 0.5% higher, on average, for the same period.

  2. How does increased corn-ethanol production affect US natural gas prices?

    Energy Technology Data Exchange (ETDEWEB)

    Whistance, Jarrett; Thompson, Wyatt [University of Missouri, Columbia, MO 65203 (United States)

    2010-05-15

    In recent years, there has been a push to increase biofuel production in the United States. The biofuel of choice, so far, has been ethanol produced from corn. The effects of increased corn-ethanol production on the consumer prices of food and energy continue to be studied and debated. This study examines, in particular, the effects of increased corn-ethanol production on US natural gas prices. A structural model of the natural gas market is developed and estimated using two stage least squares. A baseline projection for the period 2007-2018 is determined, and two scenarios are simulated. In the first scenario, current biofuel policies including EISA mandates, tariffs, and tax credits are removed. In the second scenario, we hold ethanol production to the level required only for largely obligatory additive use. The results indicate that the increased level of corn-ethanol production occurring as a result of the current US biofuel policies may lead to natural gas prices that are as much as 0.25% higher, on average, than if no biofuel policies were in place. A similar comparison between the baseline and second scenario indicates natural gas prices could be as much as 0.5% higher, on average, for the same period. (author)

  3. Genomic charting of ribosomally synthesized natural product chemical space facilitates targeted mining.

    Science.gov (United States)

    Skinnider, Michael A; Johnston, Chad W; Edgar, Robyn E; Dejong, Chris A; Merwin, Nishanth J; Rees, Philip N; Magarvey, Nathan A

    2016-10-18

    Microbial natural products are an evolved resource of bioactive small molecules, which form the foundation of many modern therapeutic regimes. Ribosomally synthesized and posttranslationally modified peptides (RiPPs) represent a class of natural products which have attracted extensive interest for their diverse chemical structures and potent biological activities. Genome sequencing has revealed that the vast majority of genetically encoded natural products remain unknown. Many bioinformatic resources have therefore been developed to predict the chemical structures of natural products, particularly nonribosomal peptides and polyketides, from sequence data. However, the diversity and complexity of RiPPs have challenged systematic investigation of RiPP diversity, and consequently the vast majority of genetically encoded RiPPs remain chemical "dark matter." Here, we introduce an algorithm to catalog RiPP biosynthetic gene clusters and chart genetically encoded RiPP chemical space. A global analysis of 65,421 prokaryotic genomes revealed 30,261 RiPP clusters, encoding 2,231 unique products. We further leverage the structure predictions generated by our algorithm to facilitate the genome-guided discovery of a molecule from a rare family of RiPPs. Our results provide the systematic investigation of RiPP genetic and chemical space, revealing the widespread distribution of RiPP biosynthesis throughout the prokaryotic tree of life, and provide a platform for the targeted discovery of RiPPs based on genome sequencing.

  4. Examination of Marine-Based Cultivation of Three Demosponges for Acquiring Bioactive Marine Natural Products

    Directory of Open Access Journals (Sweden)

    Micha Ilan

    2011-11-01

    Full Text Available Marine sponges are an extremely rich and important source of natural products. Mariculture is one solution to the so-called “supply problem” that often hampers further studies and development of novel compounds from sponges. We report the extended culture (767 days at sea in depths of 10 and 20 m of three sponge species: Negombata magnifica, Amphimedon chloros and Theonella swinhoei that produce latrunculin-B, halitoxin and swinholide-A, respectively. Since sponge-associated microorganisms may be the true producers of many of the natural products found in sponges and also be linked to the health of the sponges, we examined the stability of the bacterial communities in cultured versus wild sponges. Growth rate of the sponges (ranging from 308 to 61 and −19 (%(year−1 in N. magnifica, A. chloros and T. swinhoei, respectively differed significantly between species but not between the two depths at which the species were cultivated. Survivorship varied from 96% to 57%. During culture all species maintained the content of the desired natural product. Denaturing gradient gel electrophoresis analysis of the sponge-associated bacterial consortia revealed that differences existed between cultured and wild sponges in T. swinhoei and A. chloros but the communities remained quite stable in N. magnifica. The cultivation technique for production of natural products was found to be most appropriate for N. magnifica, while for T. swinhoei and A. chloros it was less successful, because of poorer growth and survival rates and shifts in their bacterial consortia.

  5. Examination of marine-based cultivation of three demosponges for acquiring bioactive marine natural products.

    Science.gov (United States)

    Bergman, Oded; Mayzel, Boaz; Anderson, Matthew A; Shpigel, Muki; Hill, Russell T; Ilan, Micha

    2011-01-01

    Marine sponges are an extremely rich and important source of natural products. Mariculture is one solution to the so-called "supply problem" that often hampers further studies and development of novel compounds from sponges. We report the extended culture (767 days) at sea in depths of 10 and 20 m of three sponge species: Negombata magnifica, Amphimedon chloros and Theonella swinhoei that produce latrunculin-B, halitoxin and swinholide-A, respectively. Since sponge-associated microorganisms may be the true producers of many of the natural products found in sponges and also be linked to the health of the sponges, we examined the stability of the bacterial communities in cultured versus wild sponges. Growth rate of the sponges (ranging from 308 to 61 and -19 (%)(year(-1)) in N. magnifica, A. chloros and T. swinhoei, respectively) differed significantly between species but not between the two depths at which the species were cultivated. Survivorship varied from 96% to 57%. During culture all species maintained the content of the desired natural product. Denaturing gradient gel electrophoresis analysis of the sponge-associated bacterial consortia revealed that differences existed between cultured and wild sponges in T. swinhoei and A. chloros but the communities remained quite stable in N. magnifica. The cultivation technique for production of natural products was found to be most appropriate for N. magnifica, while for T. swinhoei and A. chloros it was less successful, because of poorer growth and survival rates and shifts in their bacterial consortia.

  6. Quantifying methane emissions from natural gas production in north-eastern Pennsylvania

    Directory of Open Access Journals (Sweden)

    Z. R. Barkley

    2017-11-01

    Full Text Available Natural gas infrastructure releases methane (CH4, a potent greenhouse gas, into the atmosphere. The estimated emission rate associated with the production and transportation of natural gas is uncertain, hindering our understanding of its greenhouse footprint. This study presents a new application of inverse methodology for estimating regional emission rates from natural gas production and gathering facilities in north-eastern Pennsylvania. An inventory of CH4 emissions was compiled for major sources in Pennsylvania. This inventory served as input emission data for the Weather Research and Forecasting model with chemistry enabled (WRF-Chem, and atmospheric CH4 mole fraction fields were generated at 3 km resolution. Simulated atmospheric CH4 enhancements from WRF-Chem were compared to observations obtained from a 3-week flight campaign in May 2015. Modelled enhancements from sources not associated with upstream natural gas processes were assumed constant and known and therefore removed from the optimization procedure, creating a set of observed enhancements from natural gas only. Simulated emission rates from unconventional production were then adjusted to minimize the mismatch between aircraft observations and model-simulated mole fractions for 10 flights. To evaluate the method, an aircraft mass balance calculation was performed for four flights where conditions permitted its use. Using the model optimization approach, the weighted mean emission rate from unconventional natural gas production and gathering facilities in north-eastern Pennsylvania approach is found to be 0.36 % of total gas production, with a 2σ confidence interval between 0.27 and 0.45 % of production. Similarly, the mean emission estimates using the aircraft mass balance approach are calculated to be 0.40 % of regional natural gas production, with a 2σ confidence interval between 0.08 and 0.72 % of production. These emission rates as a percent of production are

  7. Process synthesis for natural products from plants based on PAT methodology

    DEFF Research Database (Denmark)

    Malwade, Chandrakant Ramkrishna; Qu, Haiyan; Rong, Ben-Guang

    2017-01-01

    Natural products are defined as secondary metabolites produced by plants and form a vast pool of compounds with unlimited chemical and functional diversity. Many of these secondary metabolites are high value added chemicals that are frequently used as ingredients in food, cosmetics, pharmaceuticals...... and other consumer products. Therefore, process technology towards industrial scale production of such high value chemicals from plants has significant importance. In this chapter, a process synthesis methodology for recovery of natural products from plants at conceptual level is discussed. The methodology...... (QbD) approach, has been included at various steps to obtain molecular level information of process streams and thereby, support the rational decision making. The formulated methodology has been used to isolate and purify artemisinin, an antimalarial drug, from dried leaves of the plant Artemisia...

  8. Economic growth to raise U.S. oil products, natural gas demand

    International Nuclear Information System (INIS)

    Beck, R.J.

    1994-01-01

    An accelerating economy will raise consumption of oil products and natural gas in the US this year. Contributing to demand growth will be the slump that began late last year in prices for crude oil and petroleum products. Some price recovery is likely in 1994, but there's little reason to expect a major increase. With oil production falling and demand rising, imports will have to climb again this year. OGJ projects a 2.6% increase this year following a 6.6% increase last year. Imports are expected to fill a record high 49.3% of US oil demand this year. The paper discusses energy and the economy, overall energy use, energy by source, the electrification trend, energy supplies, imports, refining operations, the growth of margins, and the energy demand of motor gasoline, jet fuel, distillate fuels, residual fuel oils, other petroleum products, and natural gas

  9. Tolerance of natural baby skin-care products on healthy, full-term infants and toddlers.

    Science.gov (United States)

    Coret, Catherine D; Suero, Michael B; Tierney, Neena K

    2014-01-01

    To evaluate the tolerance of baby skin-care products with at least 95% naturally derived ingredients on infants and toddlers. Healthy, full-term infants and toddlers aged 1-36 months were enrolled. In study 1, a lightly fragranced natural baby hair and body wash (n=30), a lightly fragranced natural baby shampoo (n=30), or a lightly fragranced natural baby lotion (n=32) were assessed over 2 weeks. In study 2, a lightly fragranced natural baby hair and body wash and a lightly fragranced natural baby lotion (n=33) were assessed as a regimen over 4 weeks. The wash and shampoo were used three or more times per week, but not more than once daily. Lotions were applied in the morning or after a bath. Clinicians assessed the arms, legs, torso, or scalp for erythema, dryness, peeling/flakiness (study 1 only), tactile roughness, edema (study 1 only), rash/irritation (study 2 only), and overall skin condition (study 2 only) at baseline, week 1, and weeks 2 or 4. Parents completed skin assessment questionnaires. In study 2, stratum corneum hydration was measured. Subjects were monitored for adverse events. No significant changes in clinical grading scores were observed, indicating that all products were well tolerated. By the end of each study, >90% of parents/caregivers believed each product was mild and gentle. In study 2, improvement in stratum corneum hydration was observed (+37% at week 1 and +48% at week 4, Pproduct-related adverse events. The natural baby skin-care products were well tolerated by infants and toddlers when used alone or as part of a skin-care regimen.

  10. Anti-Candida albicans natural products, sources of new antifungal drugs: A review.

    Science.gov (United States)

    Zida, A; Bamba, S; Yacouba, A; Ouedraogo-Traore, R; Guiguemdé, R T

    2017-03-01

    Candida albicans is the most prevalent fungal pathogen in humans. Due to the development of drug resistance, there is today a need for new antifungal agents for the efficient management of C. albicans infections. Therefore, we reviewed antifungal activity, mechanisms of action, possible synergism with antifungal drugs of all natural substances experimented to be efficient against C. albicans for future. An extensive and systematic review of the literature was undertaken and all relevant abstracts and full-text articles analyzed and included in the review. A total of 111 documents were published and highlighted 142 anti-C. albicans natural products. These products are mostly are reported in Asia (44.37%) and America (28.17%). According to in vitro model criteria, from the 142 natural substances, antifungal activity can be considered as important for 40 (28.20%) and moderate for 24 (16.90%). Sixteen products have their antifungal activity confirmed by in vivo gold standard experimentation. Microbial natural products, source of antifungals, have their antifungal mechanism well described in the literature: interaction with ergosterol (polyenes), inhibition 1,3-β-d-glucan synthase (Echinocandins), inhibition of the synthesis of cell wall components (chitin and mannoproteins), inhibition of sphingolipid synthesis (serine palmitoyltransferase, ceramide synthase, inositol phosphoceramide synthase) and inhibition of protein synthesis (sordarins). Natural products from plants mostly exert their antifungal effects by membrane-active mechanism. Some substances from arthropods are also explored to act on the fungal membrane. Interestingly, synergistic effects were found between different classes of natural products as well as between natural products and azoles. Search for anti-C. albicans new drugs is promising since the list of natural substances, which disclose activity against this yeast is today long. Investigations must be pursued not only to found more new anti

  11. Production Of Hollow Toy Product From Radiation Pre vulcanized Natural Rubber Latex (RVNRL) By Using Casting And Moulding Technique

    International Nuclear Information System (INIS)

    Mohd Noorwadi Mat Lazim; Sofian Ibrahim; Muhammad Saiful Omar

    2013-01-01

    Hollow toy products are very synonym to the child from the age of months since it able to stimulating each of their sense such as sight, hearing, taste, touch and smell. Most of hollow toy products are made from natural rubber latex by using moulding and casting technique. The moulding and casting technique is a manufacturing process by pored liquid latex into a mould, which contain cavity of the desired shape. The mould made from plaster of Paris able to absorbs water from latex meanwhile the presence of calcium ions from plaster of Paris will tend to diffuse into latex thus promote formation of deposit on surface of cavity mould. To improve the quality and safety of hollow toy product made from latex, Radiation Pre vulcanized Natural Rubber Latex (RVNRL) has been identified to be used because it can fulfill the standard requirement for latex and also due to its special abilities such as lower modulus (soft latex products), nitrosamines free, low in nitrosatables, free from chemical accelerators induced allergies and better biodegradability. This paper identify the problem appears from the process of making hollow toy products from RVNRL by using moulding and casting technique. (author)

  12. Large-Scale Biotechnological Production of the Antileukemic Marine Natural Product Sorbicillactone A

    Directory of Open Access Journals (Sweden)

    Johannes F. Imhoff

    2007-06-01

    Full Text Available In the search for novel bioactive compounds from sponge-derived microorganisms, we have recently identified two structurally and biosynthetically unprecedented fungal metabolites, the novel-type alkaloids sorbicillactone A and sorbicillactone B. Sorbicillactone A is active against leukemia cells without showing notable cytotoxicity. Therefore, we have developed an efficient process for its biotechnological production and isolation on a large scale supplying sufficient material for the ongoing preclinical investigations and structure-activity relationship (SAR studies.

  13. Life Cycle Assessment Of Hydrogen Production From Natural Gas Reforming Process

    International Nuclear Information System (INIS)

    Ozturk, M.

    2010-01-01

    Society has become concerned about the issues of natural resource depletion and environmental degradation. The environmental performance of products or processes has become a key issue, which is why ways to minimize the effects on the environment are investigated. The most effective tool for this purpose is called life cycle assessment (LCA). This concept considers the entire life cycle of product or process. The life cycle of a product begins with the extraction of raw materials from the earth to create the product and ends at the point when all materials are returned to the earth. LCA makes it possible to estimate the cumulative environmental impacts resulting from all stages in the product life cycle, often including impacts not considered in more traditional analyses. Therefore, LCA provides a comprehensive view of the environmental aspects of the product or process and a more accurate picture of the true environmental trade-offs in product selection. In the case of this study, life cycle assessments of hydrogen production via natural gas reforming process are investigated for environmental affect.

  14. Spirolactones: Recent Advances in Natural Products, Bioactive Compounds and Synthetic Strategies.

    Science.gov (United States)

    Quintavalla, Arianna

    2018-01-01

    The spirocyclic compounds have always aroused a great interest because this motif is present as structural core in a number of natural products and bioactive compounds. In particular, the spirolactone moiety has been recognized in a wide array of natural and non-natural scaffolds showing a variety of useful pharmacological properties. Extensive literature search using SciFinder (Databases: CA Plus, CAS Registry, CAS React, Chemlist, Chemcat and Medline) and Web of Science (Database: Web of Science Core Collection) was conducted. Nowadays, many efforts are being devoted to the discovery of new natural products containing the promising spirolactone framework and to the disclosure of the potential bioactivities of these chemical entities. Moreover, the medicinal relevance of many spirolactones makes these scaffolds attractive targets for the design and development of innovative and efficient synthetic strategies, enabling the construction of complex and variably substituted products. This review gives an overview on the recent advances in the spirolactones field, in terms of new compounds isolated from natural sources, recently determined bioactivity profiles and innovative synthetic approaches. The collected data demonstrate the key role played by spirolactones in medicinal chemistry and the great attention still devoted by the scientific community to these compounds. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  15. Natural product modulators of human sensations and mood: molecular mechanisms and therapeutic potential.

    Science.gov (United States)

    Pluskal, Tomáš; Weng, Jing-Ke

    2018-03-05

    Humans perceive physical information about the surrounding environment through their senses. This physical information is registered by a collection of highly evolved and finely tuned molecular sensory receptors. A multitude of bioactive, structurally diverse ligands have evolved in nature that bind these molecular receptors. The complex, dynamic interactions between the ligands and the receptors lead to changes in our sensory perception or mood. Here, we review our current knowledge of natural products and their derived analogues that interact specifically with human G protein-coupled receptors, ion channels, and nuclear hormone receptors to modulate the sensations of taste, smell, temperature, pain, and itch, as well as mood and its associated behaviour. We discuss the molecular and structural mechanisms underlying such interactions and highlight cases where subtle differences in natural product chemistry produce drastic changes in functional outcome. We also discuss cases where a single compound triggers complex sensory or behavioural changes in humans through multiple mechanistic targets. Finally, we comment on the therapeutic potential of the reviewed area of research and draw attention to recent technological developments in genomics, metabolomics, and metabolic engineering that allow us to tap the medicinal properties of natural product chemistry without taxing nature.

  16. From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product.

    Science.gov (United States)

    Pacher, Michael; Puchta, Holger

    2017-05-01

    Production of mutants of crop plants by the use of chemical or physical genotoxins has a long tradition. These factors induce the natural DNA repair machinery to repair damage in an error-prone way. In the case of radiation, multiple double-strand breaks (DSBs) are induced randomly in the genome, leading in very rare cases to a desirable phenotype. In recent years the use of synthetic, site-directed nucleases (SDNs) - also referred to as sequence-specific nucleases - like the CRISPR/Cas system has enabled scientists to use exactly the same naturally occurring DNA repair mechanisms for the controlled induction of genomic changes at pre-defined sites in plant genomes. As these changes are not necessarily associated with the permanent integration of foreign DNA, the obtained organisms per se cannot be regarded as genetically modified as there is no way to distinguish them from natural variants. This applies to changes induced by DSBs as well as single-strand breaks, and involves repair by non-homologous end-joining and homologous recombination. The recent development of SDN-based 'DNA-free' approaches makes mutagenesis strategies in classical breeding indistinguishable from SDN-derived targeted genome modifications, even in regard to current regulatory rules. With the advent of new SDN technologies, much faster and more precise genome editing becomes available at reasonable cost, and potentially without requiring time-consuming deregulation of newly created phenotypes. This review will focus on classical mutagenesis breeding and the application of newly developed SDNs in order to emphasize similarities in the context of the regulatory situation for genetically modified crop plants. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  17. Back to the Roots: Prediction of Biologically Active Natural Products from Ayurveda Traditional Medicine

    DEFF Research Database (Denmark)

    Polur, Honey; Joshi, Tejal; Workman, Christopher

    2011-01-01

    Ayurveda, the traditional Indian medicine is one of the most ancient, yet living medicinal traditions. In the present work, we developed an in silico library of natural products from Ayurveda medicine, coupled with structural information, plant origin and traditional therapeutic use. Following this...... basis of Ayurveda medicine and in drug repurposing....

  18. Heat and mass transfer during cooling and storage of agricultural products as influenced by natural convection

    NARCIS (Netherlands)

    Beukema, K.J.

    1980-01-01

    Three different models of bulk-stored agricultural products with air flow through the bulk, predicting the temperature profiles or the velocity of natural convection, are developed. The temperature distribution in a cylindrical container with insulated walls and open top and bottom, filled

  19. Kainic Acid-Induced Excitotoxicity Experimental Model: Protective Merits of Natural Products and Plant Extracts

    Directory of Open Access Journals (Sweden)

    Nur Shafika Mohd Sairazi

    2015-01-01

    Full Text Available Excitotoxicity is well recognized as a major pathological process of neuronal death in neurodegenerative diseases involving the central nervous system (CNS. In the animal models of neurodegeneration, excitotoxicity is commonly induced experimentally by chemical convulsants, particularly kainic acid (KA. KA-induced excitotoxicity in rodent models has been shown to result in seizures, behavioral changes, oxidative stress, glial activation, inflammatory mediator production, endoplasmic reticulum stress, mitochondrial dysfunction, and selective neurodegeneration in the brain upon KA administration. Recently, there is an emerging trend to search for natural sources to combat against excitotoxicity-associated neurodegenerative diseases. Natural products and plant extracts had attracted a considerable amount of attention because of their reported beneficial effects on the CNS, particularly their neuroprotective effect against excitotoxicity. They provide significant reduction and/or protection against the development and progression of acute and chronic neurodegeneration. This indicates that natural products and plants extracts may be useful in protecting against excitotoxicity-associated neurodegeneration. Thus, targeting of multiple pathways simultaneously may be the strategy to maximize the neuroprotection effect. This review summarizes the mechanisms involved in KA-induced excitotoxicity and attempts to collate the various researches related to the protective effect of natural products and plant extracts in the KA model of neurodegeneration.

  20. Microalgae in the postgenomic era: a blooming reservoir for new natural products.

    Science.gov (United States)

    Sasso, Severin; Pohnert, Georg; Lohr, Martin; Mittag, Maria; Hertweck, Christian

    2012-07-01

    Bacteria, fungi, algae and higher plants are the most prolific producers of natural products (secondary metabolites). Compared to macroalgae, considerably fewer natural products have been isolated from microalgae, which offer the possibility of obtaining sufficient and well-defined biological material from laboratory cultures. Interest in microalgae is reinforced by large-scale data sets from genome sequencing projects and the development of genetic tools such as transformation protocols. This review highlights what is currently known about the biosynthesis and biological role of natural products in microalgae, with examples from isoprenoids, complex polyketides, nonribosomal peptides, polyunsaturated fatty acids and oxylipins, alkaloids, and aromatic secondary metabolites. In addition, we introduce a bioinformatic analysis of available genome sequences from totally 16 microalgae, belonging to the green and red algae, heterokonts and haptophytes. The results suggest that the biosynthetic potential of microalgae is underestimated and many microalgal natural products remain to be discovered. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  1. Total synthesis and related studies of large, strained, and bioactive natural products

    Science.gov (United States)

    HIRAMA, Masahiro

    2016-01-01

    Our chemical syntheses and related scientific investigations of natural products with complex architectures and powerful biological activities are described, focusing on the very large 3 nm-long polycyclic ethers called the ciguatoxins, highly strained and labile chromoprotein antitumor antibiotics featuring nine-membered enediyne cores, and extremely potent anthelmintic macrolides called the avermectins. PMID:27725470

  2. Mathematical Model for Supercritical Fluid Extraction of Natural Products and Extraction Curve Evaluation

    Czech Academy of Sciences Publication Activity Database

    Sovová, Helena

    2005-01-01

    Roč. 33, č. 1 (2005), s. 35-52 ISSN 0896-8446 R&D Projects: GA AV ČR IAA4072102 Institutional research plan: CEZ:AV0Z4072921 Keywords : supercritical extraction * natural products * model Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 2.144, year: 2005

  3. Learning Organic Chemistry Through Natural Products -R-ES-O-N-A ...

    Indian Academy of Sciences (India)

    whereas the latter could form part of the laboratory curriculum. In part 1 of the series, a simple thin-layer chromatographic experiment was demonstrated. In this article we describe the procedures for the isolation of some natural products. MallY simple experiments using the isolated molecules that can be demonstrated in the ...

  4. Cost Effectiveness of Natural Regeneration for Sustaining Production Continuity in Commercial Pine Plantations

    Science.gov (United States)

    T.R. Clason

    2002-01-01

    Reforestation is a key to production continuity in commercial pine plantations. Although natural and artificial regeneration methods have been used successfully for pine seedling establishment, it is seedling growth during early stage of plantation development that affects the financial potential of a pine plantation. A study was initiated to determine the effect of...

  5. Evaluating natural resource amenities in a human life expectancy production function

    Science.gov (United States)

    Neelam C. Poudyal; Donald G. Hodges; J.M. Bowker; H.K. Cordell

    2009-01-01

    This study examined the effect of natural resource amenities on human life expectancy. Extending theexisting model of the life expectancy production function, and correcting for spatial dependence, weevaluated the determinants of life expectancy using county level data. Results indicate that after controlling

  6. Making a Natural Product Chemistry Course Meaningful with a Mini Project Laboratory

    Science.gov (United States)

    Hakim, Aliefman; Liliasari; Kadarohman, Asep; Syah, Yana Maolana

    2016-01-01

    This paper discusses laboratory activities that can improve the meaningfulness of natural product chemistry course. These laboratory activities can be useful for students from many different disciplines including chemistry, pharmacy, and medicine. Students at the third-year undergraduate level of chemistry education undertake the project to…

  7. Discovery of novel phosphonate natural products and their biosynthetic pathways by large-scale genome mining

    Science.gov (United States)

    Genome mining has revolutionized the field of natural products, providing hope that new antibiotics can be discovered in time before all remainders are rendered useless against multidrug resistant pathogens. While this approach has been successful in academic settings focused on small collections or...

  8. Insights into natural products biosynthesis from analysis of 490 polyketide synthases from Fusarium

    Science.gov (United States)

    Species of the fungus Fusarium collectively cause disease on almost all crop plants and produce numerous natural products (NPs), including some of the mycotoxins of greatest concern to agriculture. Many Fusarium NPs are derived from polyketide synthases, large multi-domain enzymes that catalyze sequ...

  9. Current status and prospects of computational resources for natural product dereplication: a review.

    Science.gov (United States)

    Mohamed, Ahmed; Nguyen, Canh Hao; Mamitsuka, Hiroshi

    2016-03-01

    Research in natural products has always enhanced drug discovery by providing new and unique chemical compounds. However, recently, drug discovery from natural products is slowed down by the increasing chance of re-isolating known compounds. Rapid identification of previously isolated compounds in an automated manner, called dereplication, steers researchers toward novel findings, thereby reducing the time and effort for identifying new drug leads. Dereplication identifies compounds by comparing processed experimental data with those of known compounds, and so, diverse computational resources such as databases and tools to process and compare compound data are necessary. Automating the dereplication process through the integration of computational resources has always been an aspired goal of natural product researchers. To increase the utilization of current computational resources for natural products, we first provide an overview of the dereplication process, and then list useful resources, categorizing into databases, methods and software tools and further explaining them from a dereplication perspective. Finally, we discuss the current challenges to automating dereplication and proposed solutions. © The Author 2015. Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com.

  10. Biological evaluation of tubulysin A: A potential anticancer and antiangiogenic natural product

    NARCIS (Netherlands)

    Kaur, Gurmeet; Hollingshead, Melinda; Holbeck, Susan; Schauer-Vukašinović, Vesna; Camalier, Richard F.; Dömling, Alexander; Agarwal, Seema

    2006-01-01

    Tubulysin A (tubA) is a natural product isolated from a strain of myxobacteria that has been shown to depolymerize microtubules and induce mitotic arrest. The potential of tubA as an anticancer and antiangiogenic agent is explored in the present study. tubA shows potent antiproliferative activity in

  11. A fluorescence-based bioassay for antibacterials and its application in screening natural product extracts

    NARCIS (Netherlands)

    Michels, Katharina; Heinke, Ramona; Schöne, Pia; Kuipers, Oscar P; Arnold, Norbert; Wessjohann, Ludger A

    2015-01-01

    The reliable assessment of the biological activity of a minor component embedded in a complex matrix of several hundred compounds is a difficult but common task in the search for natural product-based antibiotics, for example, by bioassay-guided fractionation. To quantify the antibiotic properties,

  12. A roadmap for natural product discovery based on large-scale genomics and metabolomics

    Science.gov (United States)

    Actinobacteria encode a wealth of natural product biosynthetic gene clusters, whose systematic study is complicated by numerous repetitive motifs. By combining several metrics we developed a method for global classification of these gene clusters into families (GCFs) and analyzed the biosynthetic ca...

  13. Screening bioactive compounds from natural product and its preparations using capillary electrophoresis.

    Science.gov (United States)

    Ma, Huifen; Bai, Yun; Li, Jin; Chang, Yan-Xu

    2018-01-01

    Bioactive ingredients of natural products can protect human body from harm, as well as prevent and treat disease. Screening bioactive compounds from natural products is attracting particular attention. It is a great challenge to separate and detect the active compounds from complex matrix natural products. CE plays a vital role in screening active compounds because of its unique features such as high-efficiency separation, short-analysis time, minimal sample consumption, and ease to realize automatization etc. Additionally, CE has been developed various modes owing to its abundant advantages in analysis and separation of compounds. The purpose of this work is to review previous developments and applications of CE in screening bioactive compounds derived from natural products from 2007 to 2017. This review does not only summarize the traditional methods of detecting active ingredients but also briefly introduces some novel assays. The trends in the application of CE in active compounds screening are addressed in the article. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Computational genomic identification and functional reconstitution of plant natural product biosynthetic pathways

    NARCIS (Netherlands)

    Medema, Marnix H.; Osbourn, Anne

    2016-01-01

    Covering: 2003 to 2016 The last decade has seen the first major discoveries regarding the genomic basis of plant natural product biosynthetic pathways. Four key computationally driven strategies have been developed to identify such pathways, which make use of physical clustering, co-expression,

  15. Isolation of Betulin and Rearrangement to Allobetulin: A Biomimetic Natural Product Synthesis

    Science.gov (United States)

    Green, Brian; Bentley, Michael D.; Chung, Bong Y.; Lynch, Nicholas G.; Jensen, Bruce L.

    2007-01-01

    The triterpenes are a diverse class of widely distributed natural products derived from squalene. Various cyclization and subsequent rearrangement reactions produce many complex structural types. These compounds frequently display a wide divergence of biological properties. For example the pentacyclic triterpene, betulin, is isolated from white…

  16. Nature-inspired design strategies in sustainable product development : A case study of student projects

    NARCIS (Netherlands)

    De Pauw, I.C.; Karana, E.; Kandachar, P.V.

    2012-01-01

    In design practice, Nature-Inspired Design Strategies (NIDS) can be applied when developing sustainable products. However, knowledge on how this actually helps designers is lacking. This study explores the effects of applying Cradle to Cradle and Biomimicry in student projects, as compared to using

  17. Natural Products for the Prevention and Alleviation of Risk Factors for Diabetes: Chromium and Cinnamon

    Science.gov (United States)

    Natural products are widespread for the alleviation and prevention of the risk factors of the metabolic syndrome and diabetes. We have shown that glucose, insulin, cholesterol, and hemoglobin A1c levels are all improved in people with type 2 diabetes following chromium supplementation in a double-b...

  18. Natural Products Research in South Africa: End of an Era on Land or ...

    African Journals Online (AJOL)

    South African organic chemistry is deeply rooted in a rich history of natural product chemistry research with the secondary metabolites emanating from South Africa's unique floral kingdoms dominating this research for nearly a century. However, South Africa's terrestrial biodiversity is exceeded by an even greater diversity of ...

  19. Dimensional Reduction in Vector Space Methods for Natural Language Processing: Products and Projections

    Science.gov (United States)

    Aerts, Sven

    2011-12-01

    We introduce vector space based approaches to natural language processing and some of their similarities with quantum theory when applied to information retrieval. We explain how dimensional reduction is called for from both a practical and theoretical point of view and how this can be achieved through choice of product or through projectors onto subspaces.

  20. Perturbing dissimilar biomolecular targets from natural product scaffolds and focused chemical decoration

    DEFF Research Database (Denmark)

    Nielsen, John; Tung, Truong Thanh; Tim, Holm Jakobsen

    agents. On first attempt, by screening natural product sources we have successfully discovered that curcuminoids as potent inhibitors of p-type ATPases from diverse kingdoms of life including Pma1. On other attempt, the fungal metabolite fusaric acid was reported to reduce stomatal conductance in banana...

  1. The Role of Natural Products in Drug Discovery and Development against Neglected Tropical Diseases

    Directory of Open Access Journals (Sweden)

    Peter Mubanga Cheuka

    2016-12-01

    Full Text Available Endemic in 149 tropical and subtropical countries, neglected tropical diseases (NTDs affect more than 1 billion people annually, including 875 million children in developing economies. These diseases are also responsible for over 500,000 deaths per year and are characterized by long-term disability and severe pain. The impact of the combined NTDs closely rivals that of malaria and tuberculosis. Current treatment options are associated with various limitations including widespread drug resistance, severe adverse effects, lengthy treatment duration, unfavorable toxicity profiles, and complicated drug administration procedures. Natural products have been a valuable source of drug regimens that form the cornerstone of modern pharmaceutical care. In this review, we highlight the potential that remains untapped in natural products as drug leads for NTDs. We cover natural products from plant, marine, and microbial sources including natural-product-inspired semi-synthetic derivatives which have been evaluated against the various causative agents of NTDs. Our coverage is limited to four major NTDs which include human African trypanosomiasis (sleeping sickness, leishmaniasis, schistosomiasis and lymphatic filariasis.

  2. Application of cyclic phosphonamide reagents in the total synthesis of natural products and biologically active molecules

    Directory of Open Access Journals (Sweden)

    Thilo Focken

    2014-08-01

    Full Text Available A review of the synthesis of natural products and bioactive compounds adopting phosphonamide anion technology is presented highlighting the utility of phosphonamide reagents in stereocontrolled bond-forming reactions. Methodologies utilizing phosphonamide anions in asymmetric alkylations, Michael additions, olefinations, and cyclopropanations will be summarized, as well as an overview of the synthesis of the employed phosphonamide reagents.

  3. Are agricultural and natural sources of bio-products important for modern regenerative medicine? A review

    Directory of Open Access Journals (Sweden)

    Maciej Nowacki

    2017-05-01

    Evolving trends in agriculture are likely to play a key role in the future development of a number of systemic and local medical procedures within tissue engineering and regenerative medicine. This is in addition to the use of bio-products derived from the natural environment which are found to deliver positive results in the treatment of prospective patients.

  4. Development of Antiatherosclerotic Drugs on the basis of Natural Products Using Cell Model Approach

    Directory of Open Access Journals (Sweden)

    Alexander N. Orekhov

    2015-01-01

    Full Text Available Atherosclerosis including its subclinical form is one of the key medical and social problems. At present, there is no therapy available for widespread use against subclinical atherosclerosis. The use of synthetic drugs for the prevention of arteriosclerosis in its early stages is not sufficient because of the limited indications for severe side effects and high cost of treatment. Obviously, effective antiatherosclerotic drugs based on natural products would be a preferred alternative. Simple cell-based models for testing different natural products have been developed and the ability of natural products to prevent intracellular lipid accumulation in primary cell culture was evaluated. This approach utilizing cell models allowed to test effects of such direct antiatherosclerotic therapy, analyzing the effects mimicking those which can occur “at the level” of arterial wall via the inhibition of intracellular lipid deposition. The data from the carried out clinical trials support a point of view that the identification of antiatherosclerotic activity of natural products might offer a great opportunity for the prevention and treatment of atherosclerotic disease, reducing cardiovascular morbidity and mortality.

  5. Recent applications of intramolecular Diels-Alder reactions to natural product synthesis

    DEFF Research Database (Denmark)

    Juhl, M.; Tanner, David Ackland

    2009-01-01

    This tutorial review presents some recent examples of intramolecular Diels-Alder (IMDA) reactions as key complexity-generating steps in the total synthesis of structurally intricate natural products. The opportunities afforded by transannular (TADA) versions of the IMDA reaction in complex molecule...

  6. Evaluating natural resource amenities in a human life expectancy production function

    Science.gov (United States)

    Neelam C. Poudyal; Donald G. Hodges; J.M. Bowker; H.K. Cordell

    2009-01-01

    This study examined the effect of natural resource amenities on human life expectancy. Extending the existing model of the life expectancy production function, and correcting for spatial dependence, we evaluated the determinants of life expectancy using county level data. Results indicate that after controlling for socio-demographic and economic factors, medical...

  7. Metabolomics-based chemotaxonomy of root endophytic fungi for natural products discovery.

    Science.gov (United States)

    Maciá-Vicente, Jose G; Shi, Yan-Ni; Cheikh-Ali, Zakaria; Grün, Peter; Glynou, Kyriaki; Kia, Sevda Haghi; Piepenbring, Meike; Bode, Helge B

    2018-03-01

    Fungi are prolific producers of natural products routinely screened for biotechnological applications, and those living endophytically within plants attract particular attention because of their purported chemical diversity. However, the harnessing of their biosynthetic potential is hampered by a large and often cryptic phylogenetic and ecological diversity, coupled with a lack of large-scale natural products' dereplication studies. To guide efforts to discover new chemistries among root-endophytic fungi, we analyzed the natural products produced by 822 strains using an untargeted UPLC-ESI-MS/MS-based approach and linked the patterns of chemical features to fungal lineages. We detected 17 809 compounds of which 7951 were classified in 1992 molecular families, whereas the remaining were considered unique chemistries. Our approach allowed to annotate 1191 compounds with different degrees of accuracy, many of which had known fungal origins. Approximately 61% of the compounds were specific of a fungal order, and differences were observed across lineages in the diversity and characteristics of their chemistries. Chemical profiles also showed variable chemosystematic values across lineages, ranging from relative homogeneity to high heterogeneity among related fungi. Our results provide an extensive resource to dereplicate fungal natural products and may assist future discovery programs by providing a guide for the selection of target fungi. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

  8. Matrix Metalloproteinase Inhibitors (MMPIs from Marine Natural Products: the Current Situation and Future Prospects

    Directory of Open Access Journals (Sweden)

    Se-Kwon Kim

    2009-03-01

    Full Text Available Matrix metalloproteinases (MMPs are a family of more than twenty five secreted and membrane-bound zinc-endopeptidases which can degrade extracellular matrix (ECM components. They also play important roles in a variety of biological and pathological processes. Matrix metalloproteinase inhibitors (MMPIs have been identified as potential therapeutic candidates for metastasis, arthritis, chronic inflammation and wrinkle formation. Up to present, more than 20,000 new compounds have been isolated from marine organisms, where considerable numbers of these naturally occurring derivatives are developed as potential candidates for pharmaceutical application. Eventhough the quantity of marine derived MMPIs is less when compare with the MMPIs derived from terrestrial materials, huge potential for bioactivity of these marine derived MMPIs has lead to large number of researches. Saccharoids, flavonoids and polyphones, fatty acids are the most important groups of MMPIs derived from marine natural products. In this review we focus on the progress of MMPIs from marine natural products.

  9. Exploring fungal biodiversity for the production of water-soluble pigments as potential natural food colorants

    DEFF Research Database (Denmark)

    Mapari, Sameer Shamsuddin; Nielsen, Kristian Fog; Larsen, Thomas Ostenfeld

    2005-01-01

    The production of many currently authorized natural food colorants has a number of disadvantages, including a dependence on the supply of raw materials and variations in pigment extraction. Fungi provide a readily available alternative source of naturally derived food colorants that could easily...... be produced in high yields. The recent authorization of a fungal food colorant has fuelled research to explore the extraordinary chemical diversity and biodiversity of fungi for the biotechnological production of pigments as natural food colorants. These studies require an appropriate use of chernotaxonomic...... tools and a priori knowledge of fungal metabolites to carry out intelligent screening for known or novel colorants as lead compounds. Such screening would result in the preselection of some potential pigment producers and the deselection of pathogenic strains and toxin producers. With advances in gene...

  10. Expansion of chemical space for natural products by uncommon P450 reactions.

    Science.gov (United States)

    Zhang, Xingwang; Li, Shengying

    2017-08-30

    Covering: 2000 to 2017Cytochrome P450 enzymes (P450s) are the most versatile biocatalysts in nature. The catalytic competence of these extraordinary hemoproteins is broadly harnessed by numerous chemical defenders such as bacteria, fungi, and plants for the generation of diverse and complex natural products. Rather than the common tailoring reactions (e.g. hydroxylation and epoxidation) mediated by the majority of biosynthetic P450s, in this review, we will focus on the unusual P450 enzymes in relation to new chemistry, skeleton construction, and structure re-shaping via their own unique catalytic power or the intriguing protein-protein interactions between P450s and other proteins. These uncommon P450 reactions lead to a higher level of chemical space expansion for natural products, through which a broader spectrum of bioactivities can be gained by the host organisms.

  11. An Overview of Natural Gas Conversion Technologies for Co-Production of Hydrogen and Value-Added Solid Carbon Products

    Energy Technology Data Exchange (ETDEWEB)

    Dagle, Robert A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Dagle, Vanessa [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Bearden, Mark D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Holladay, Jamelyn D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Krause, Theodore R. [Argonne National Lab. (ANL), Argonne, IL (United States); Ahmed, Shabbir [Argonne National Lab. (ANL), Argonne, IL (United States)

    2017-11-16

    This report was prepared in response to the U.S. Department of Energy Fuel Cell Technologies Office Congressional Appropriation language to support research on carbon-free production of hydrogen using new chemical processes that utilize natural gas to produce solid carbon and hydrogen. The U.S. produces 9-10 million tons of hydrogen annually with more than 95% of the hydrogen produced by steam-methane reforming (SMR) of natural gas. SMR is attractive because of its high hydrogen yield; but it also converts the carbon to carbon dioxide. Non-oxidative thermal decomposition of methane to carbon and hydrogen is an alternative to SMR and produces CO2-free hydrogen. The produced carbon can be sold as a co-product, thus providing economic credit that reduces the delivered net cost of hydrogen. The combination of producing hydrogen with potentially valuable carbon byproducts has market value in that this allows greater flexibility to match the market prices of hydrogen and carbon. That is, the higher value product can subsidize the other in pricing decisions. In this report we highlight the relevant technologies reported in the literature—primarily thermochemical and plasma conversion processes—and recent research progress and commercial activities. Longstanding technical challenges include the high energetic requirements (e.g., high temperatures and/or electricity requirements) necessary for methane activation and, for some catalytic processes, the separation of solid carbon product from the spent catalyst. We assess current and new carbon product markets that could be served given technological advances, and we discuss technical barriers and potential areas of research to address these needs. We provide preliminary economic analysis for these processes and compare to other emerging (e.g., electrolysis) and conventional (e.g., SMR) processes for hydrogen production. The overarching conclusion of this study is that the cost of hydrogen can be potentially

  12. Fermentation-Guided Natural Products Isolation of a Grape Berry Triacylglyceride that Enhances Ethyl Ester Production

    Directory of Open Access Journals (Sweden)

    Christopher L. Blackford

    2018-01-01

    Full Text Available A full understanding of the origin, formation and degradation of volatile compounds that contribute to wine aroma is required before wine style can be effectively managed. Fractionation of grapes represents a convenient and robust method to simplify the grape matrix to enhance our understanding of the grape contribution to volatile compound production during yeast fermentation. In this study, acetone extracts of both Riesling and Cabernet Sauvignon grape berries were fractionated and model wines produced by spiking aliquots of these grape fractions into model grape juice must and fermented. Non-targeted SPME-GCMS analyses of the wines showed that several medium chain fatty acid ethyl esters were more abundant in wines made by fermenting model musts spiked with certain fractions. Further fractionation of the non-polar fractions and fermentation of model must after addition of these fractions led to the identification of a mixture of polyunsaturated triacylglycerides that, when added to fermenting model must, increase the concentration of medium chain fatty acid ethyl esters in wines. Dosage-response fermentation studies with commercially-available trilinolein revealed that the concentration of medium chain fatty acid ethyl esters can be increased by the addition of this triacylglyceride to model musts. This work suggests that grape triacylglycerides can enhance the production of fermentation-derived ethyl esters and show that this fractionation method is effective in segregating precursors or factors involved in altering the concentration of fermentation volatiles.

  13. Psychotropic medications from natural products: a review of promising research and recommendations.

    Science.gov (United States)

    Lake, J

    2000-05-01

    Most psychotropic agents employed in allopathic medicine have limited efficacy and significant side effects. Although usually beneficial, synthetic psychotropics are unavailable to approximately 80% of the world's population. Improved understanding of appropriate and safe uses of naturally occurring substances as psychotropic agents will greatly contribute to global mental healthcare. Empirical validation of non-allopathic treatments to ensure safety and efficacy is important because increasing numbers of patients in economically developed countries are using natural substances as medicinal agents. Patients and clinicians often lack accurate information, resulting in poor treatment outcomes or the possibility of drug-drug interactions when herbal medications are used with synthetic psychotropic medications. An important objective of this paper is to distinguish compelling scientific evidence supporting the use of natural products in psychiatry from political or institutional biases that have been misrepresented as scientific arguments. Following an overview of historical, legal, and regulatory issues, this paper presents findings of a systematic literature review on natural products used to treat neuropsychiatric disorders. Significant recent research is reviewed, including emerging treatments of seizure disorders, schizophrenia, dementia and age-related cognitive decline, depression, anxiety states, and substance abuse. Substantial evidence is advanced for safety and efficacy of many natural products used to treat neuropsychiatric symptoms or disorders. Preliminary findings suggest that several treatments based on natural substances are as effective and safe as synthetic pharmaceuticals in current use. Additional studies are indicated to confirm these findings, to elucidate mechanisms of action, and to elaborate standards for safe and appropriate treatment indications. In conclusion, strategic approaches aimed at facilitating improved networking, accelerating

  14. Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties.

    Science.gov (United States)

    Lee, Jin-Ching; Hou, Ming-Feng; Huang, Hurng-Wern; Chang, Fang-Rong; Yeh, Chi-Chen; Tang, Jen-Yang; Chang, Hsueh-Wei

    2013-06-03

    For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro and in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs.

  15. Natural Products for the Prevention and Treatment of Hangover and Alcohol Use Disorder

    Directory of Open Access Journals (Sweden)

    Fang Wang

    2016-01-01

    Full Text Available Alcoholic beverages such as beer, wine and spirits are widely consumed around the world. However, alcohol and its metabolite acetaldehyde are toxic and harmful to human beings. Chronic alcohol use disorder or occasional binge drinking can cause a wide range of health problems, such as hangover, liver damage and cancer. Some natural products such as traditional herbs, fruits, and vegetables might be potential dietary supplements or medicinal products for the prevention and treatment of the problems caused by excessive alcohol consumption. The aim of this review is to provide an overview of effective natural products for the prevention and treatment of hangover and alcohol use disorder, and special emphasis is paid to the possible functional component(s and related mechanism(s of action.

  16. Natural product biosyntheses in cyanobacteria: A treasure trove of unique enzymes.

    Science.gov (United States)

    Kehr, Jan-Christoph; Gatte Picchi, Douglas; Dittmann, Elke

    2011-01-01

    Cyanobacteria are prolific producers of natural products. Investigations into the biochemistry responsible for the formation of these compounds have revealed fascinating mechanisms that are not, or only rarely, found in other microorganisms. In this article, we survey the biosynthetic pathways of cyanobacteria isolated from freshwater, marine and terrestrial habitats. We especially emphasize modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) pathways and highlight the unique enzyme mechanisms that were elucidated or can be anticipated for the individual products. We further include ribosomal natural products and UV-absorbing pigments from cyanobacteria. Mechanistic insights obtained from the biochemical studies of cyanobacterial pathways can inspire the development of concepts for the design of bioactive compounds by synthetic-biology approaches in the future.

  17. Natural product biosyntheses in cyanobacteria: A treasure trove of unique enzymes

    Directory of Open Access Journals (Sweden)

    Jan-Christoph Kehr

    2011-12-01

    Full Text Available Cyanobacteria are prolific producers of natural products. Investigations into the biochemistry responsible for the formation of these compounds have revealed fascinating mechanisms that are not, or only rarely, found in other microorganisms. In this article, we survey the biosynthetic pathways of cyanobacteria isolated from freshwater, marine and terrestrial habitats. We especially emphasize modular nonribosomal peptide synthetase (NRPS and polyketide synthase (PKS pathways and highlight the unique enzyme mechanisms that were elucidated or can be anticipated for the individual products. We further include ribosomal natural products and UV-absorbing pigments from cyanobacteria. Mechanistic insights obtained from the biochemical studies of cyanobacterial pathways can inspire the development of concepts for the design of bioactive compounds by synthetic-biology approaches in the future.

  18. Exploiting epoxidized natural rubber latex (ENRL) as a starting raw material for latex-based products

    Science.gov (United States)

    Siti Nor Qamarina, M.; Fatimah Rubaizah, M. R.; Nurul Suhaira, A.; Norhanifah, M. Y.

    2017-12-01

    Epoxidized natural rubber latex (ENRL) is a chemically modified natural rubber latex produced from epoxidation process that involves usage of organic peracids. Conversion of the ENRL into dry rubber products has been known to exhibit many beneficial properties, however limited published works were found on diversifiying the ENRL latex-based products applications. In this preliminary work, different source of raw materials and neutralization systems were investigated. The objective was to explore possibilities in producing distinctive ENRL. Findings have demonstrated that different source of raw materials and neutralization systems influenced the typical ENRL specifications, stability behavior and particle size distribution. Morphological observations performed on these ENRL systems appeared to agree with the ENRL characteristics achieved. Since experimenting these two main factors resulted in encouraging ENRL findings, detailed work shall be further scrutinized to search for an optimum condition in producing marketable ENRL specifically for latex-based products applications.

  19. Natural and synthetic products used for the treatment of smoke inhalation: a patent review.

    Science.gov (United States)

    De Carvalho, Fernanda Oliveira; Silva, Érika Ramos; Felipe, Fernanda Araújo; Teixeira, Luciana Garcez Barretto; Zago, Luciana Beatriz Silva; Nunes, Paula Santos; Shanmugam, Saravanan; Serafini, Mairim Russo; Araújo, Adriano Antunes de Souza

    2017-08-01

    Inhalation injury is the leading cause of death in burn patients and is usually caused by the uninhibited absorption of smoke, which has an extremely toxic effect on the respiratory system. The physiopathology of inhalation injury covers multiple factors and the injured respiratory system may present deterioration in a few hours. Areas covered: In this present review, we analyzed patents based on both natural and synthetic products developed for the treatment of smoke inhalation. We found 14 patents in Espacenet, 25 in WIPO and none in the USPTO data search. Expert opinion: Several previous reports on treatments used for smoke inhalation are discussed. Although there are a number of research based products for this injury, there has been no review of the patents dealing with treatments for smoke inhalation. The development of novel natural or synthetic products are discussed in detail in the review.

  20. Production of candidate natural matrix reference materials for micro-analytical techniques

    International Nuclear Information System (INIS)

    Zeisler, R.; Fajgelj, A.; Zeiller, E.

    2002-01-01

    Homogeneity is considered to be the most vital prerequisite for a certified reference material (CRM); more stringent requirements exist for the analysis of small subsamples. Many of the natural matrix CRMs are prepared from bulk samples by grinding and milling them to a certain particle size, which is expected to provide a more homogenous material; however recommended sample sizes for biological and environmental reference materials are found to be more than 100 mg. Since the milling of materials is costly and has some drawbacks, natural materials that already occur as small particles such as air particulate matter, certain sediments, and cellular biological materials may form the basis of the required reference materials. The nature of these materials, i.e. naturally occurring particles, may provide ideal model reference material. We describe here the production of the materials and preliminary tests, the evaluation for the micro-analytical techniques