WorldWideScience

Sample records for birgitta wallstedt msc

  1. S. Birgitta and mysticism

    Directory of Open Access Journals (Sweden)

    Birgit Klockars

    1970-01-01

    Full Text Available S. Birgitta (Bridget of Sweden (about 1303-1373 lived in a period of flourishing Christian mysticism. Hardly any epoch has produced as many well-known mystics in Western Europe as the 13th and 14th centuries. In Eastern Christendom mysticism also reached one of its highest points in the 14th century. The threads connecting Birgitta with other mystics and devout circles lead not only backwards, but also forwards. The Canonization Acts bear testimony to the fact that Birgitta's personality made a deep impression on many of those who met her. By her life and example, by conversation and counsels she gave others the impulses she had herself received.

  2. Birgitta festival tulekul

    Index Scriptorium Estoniae

    2006-01-01

    Birgitta festivali üritustest Tallinnas Pirita kloostri varemetes: 11. ja 12. aug. Astor Piazzolla tango-ooper "Maria de BuenosAires", 15. aug. Mozarti ooper "Tituse halastus", 17. aug. D. Shostakovitshi ooper "Mtsenski maakonna lady Macbeth", 18. aug. Carl Orffi "Carmina Burana, 19. aug. Verdi "Reekviem", 20. aug. Tõnis Mägi, Ultima Thule ja Tallinna Kammerorkestri kontsert

  3. Birgitta festival valge tiiva all / Toomas Velmet

    Index Scriptorium Estoniae

    Velmet, Toomas, 1942-

    2007-01-01

    19. augustil Pirita kloostris Birgitta festivali raames toimunud kontserdist "Tchaikovsky.Ballet@Classics.Jazz", kus osalesid Novaja Opera orkester, Imperial Ballet, Estonian Dream Big Band ja eesti jazztantsijad

  4. Birgitta festival / Heili Vaus-Tamm

    Index Scriptorium Estoniae

    Vaus-Tamm, Heili, 1961-

    2007-01-01

    Birgitta festivalil Pirita kloostris 10. - 19. augustini toimuvast lühidalt: Arthur Honeggeri oratoorium "Jeanne d'Arc tuleriidal", Astor Piazolla tango-ooper "Maria de Buenos Aires", Carl Orffi "Carmina Burana" jpt.

  5. Ülestähendusi Birgitta festivalilt 2009 / Raili Sule

    Index Scriptorium Estoniae

    Sule, Raili

    2009-01-01

    13.-23. augustini Pirita kloostri varemetes toimunud Tallinna Filharmoonia korraldatud Birgitta festivali raames toimunud Dmitri Bertmanni lavastustest - Umberto Giordano "Siber" ja Giuseppe Verdi "Falstaff"

  6. Jaht uutele elamustele : Patt ja armastus Birgitta festivali ooperietendustes / Anu Veenre

    Index Scriptorium Estoniae

    Veenre, Anu

    2011-01-01

    Birgitta festivalil etendunud G. Verdi ooperist "Attila" (avastaja Üllar Saaremäe), Moskva ooperiteatri Helikon lavastustest A. Dvoraki "Näkineid" ja G. Bizet' "Carmen" (mõlema lavastaja Dmitri Bertman)

  7. Birgitta festival anno 2011 : Ooperiklassikat moodsa kujunduse kaudu / Kristel Pappel, Harry Liivrand

    Index Scriptorium Estoniae

    Pappel, Kristel, 1961-

    2012-01-01

    Birgitta festivalist ja seal etendunud G. Verdi ooperist "Attila" (avastaja Üllar Saaremäe), Moskva ooperiteatri Helikon lavastustest A. Dvoraki "Näkineid" ja G. Bizet' "Carmen" (mõlema lavastaja Dmitri Bertman)

  8. Birgitta festival on mõtlevale ja tundvale inimesele / Heili Vaus-Tamm

    Index Scriptorium Estoniae

    Vaus-Tamm, Heili, 1961-

    2010-01-01

    Birgitta festival (12.–21. augustini) on kuue tegutsemisaasta jooksul kujundanud välja oma põhimõtted ja suunad. Festival pakub publikule põnevaid psühholoogilisi lavastusi maailma teatrikunsti tasemel, ning seda arusaadavas ja esteetilises vormis

  9. ”Att befrias fran varje horisontlinje”. Birgitta Lillpers poetiska landskap

    OpenAIRE

    Niewiarowska-Rasmussen, Ewa

    2003-01-01

    The paper constitutes an analysis of the poetry of Birgitta Lillpers in the context of the postmodern break-up of traditional definitions of literary genres. The characteristic features of the Swedish poet's work are ambiguity, fragmentation, and a diffuse persona of the speaker. Lillpers' poetry is rich in lexical, semantic and syntactic experimentation.

  10. Teater Pan-Optikum tõstab Birgitta festivali lendu / Sigrun Fritsch ; intervjueerinud Heili Vaus-Tamm

    Index Scriptorium Estoniae

    Fritsch, Sigrun

    2010-01-01

    12.-21. augustini Pirita kloostris toimuval Birgitta festivalil etendub Glucki ooper "Orpheus ja Eurydike", lavastaja Georg Rootering. Teater Pan.Optikum ("füüsiline teater" ehk Aktionstheater) osaleb lavastuses videoprojektsiooni ja lauljate karakterite dubleerimisega

  11. Oo, püha Birgitta, mis toimub augustis sinu kloostri vanade varemete vahel?

    Index Scriptorium Estoniae

    2007-01-01

    Birgitta festivalil Pirita kloostri varemetes mängukavas: Arthur Honeggeri oratoorium "Jeanne d'Arc tuleriidal" (10. ja 11. aug., lavastajaks Üllar Saaremäe, peaosas Mirtel Pohla ja Benedictuse osatäitjaks Rain Simmul), Piazolla tango-ooper "Maria de Buenos Aires" (14. aug.), Carl Orffi lavaline kantaat "Carmina Burana" (15. aug.), A. Rubinshteini ooper "Deemon" (16. aug.), lavastatud galakontsert filmikatketega ooperidiiva Maria Callasest (17. aug.), klassikalise ja dzhässballeti õhtud Vene Keiserlikult balletitrupilt (18. ja 19. aug.)

  12. Tumor Stroma Manipulation By MSC.

    Science.gov (United States)

    Grisendi, Giulia; Spano, Carlotta; Rossignoli, Filippo; D Souza, Naomi; Golinelli, Giulia; Fiori, Agnese; Horwitz, Edwin M; Guarneri, Valentina; Piacentini, Federico; Paolucci, Paolo; Dominici, Massimo

    2016-01-01

    Tumor stroma (TS) plays relevant roles in all steps of cancer development. We here address several fundamental aspects related with the interaction between cancer cells and their stromal counterparts. Dissecting these players is of pivotal importance to understand oncogenesis, immunoescape and drug resistance. In addition, this better comprehension will allow the introduction of novel and more effective therapeutic approaches where manipulated stromal elements may become detrimental for tumor growth. Our group and others rely on the use of multipotent mesenchymal stromal/stem cells (MSC) as anti-cancer tools, since these putative TS cell precursors can deliver potent apoptosis-inducing agents. Multimodal-armed MSC can target a variety of cancers in vitro and, when injected in vivo, they localize into tumors mediating cell death without evident toxicities to normal tissues. While several aspects of these strategies shall require further investigations, these approaches collectively indicate how TS manipulation by MSC represents a tool to influence the fate of cancer cells, creating a new generation of anti-cancer strategies. PMID:26953248

  13. Checking MSC Specifications for Timing Inconsistency

    Institute of Scientific and Technical Information of China (English)

    LI Xuandong(李宣东); TAN Wenkai(谭文凯); ZHENG Guoliang(郑国梁)

    2002-01-01

    Message sequence chart (MSC) is a graphical and textual language for the description and specification of the interactions between system components. MSC specifica tions allow convenient expression of multiple scenarios, and offer an intuitive and visual way of describing design requirements. Like any other aspect of the specification and design process, MSCs are amenable to errors, and their analysis is important. In this paper, the verification problem of MSC specification for timing inconsistency is studied, which means that no execu tion scenario described by an MSC specification is timing consistent. An algorithm is developed to check MSC specifications for timing inconsistency.

  14. Hungarian medical physics MSc education

    International Nuclear Information System (INIS)

    The medical physics specialisation aims at providing high level interdisciplinary theoretical and practical knowledge and readily applicable skills, which can put into action in both the clinical and the R and D field. The first competence based gradual medical physics course in the B.Sc./M.Sc. system in Hungary was launched two years ago at the Faculty of Natural Sciences of Budapest University of Technology and Economics managed by the Institute of Nuclear Techniques. The MSc programme was compiled on the base of EFOMP, IPEM, AAPM and IAEA recommendations. The course curriculum comprises fundamental physical subjects (atomic and molecular physics, nuclear physics and particle physics) as well as fundamental medical knowledge (anatomy, physiology and radiobiology) required for subjects of diagnostic and therapy. Students of this MSc branch may chose further subjects from a 'compulsory optional' set of subjects, which contains medical imaging, X-ray diagnostics, radiation therapy, magnetic resonance imaging, radiation protection, Monte Carlo calculation and its clinical applications, ultrasound diagnostics and nuclear medicine. (authors)

  15. Cellular Kinetics of Perivascular MSC Precursors

    Directory of Open Access Journals (Sweden)

    William C. W. Chen

    2013-01-01

    Full Text Available Mesenchymal stem/stromal cells (MSCs and MSC-like multipotent stem/progenitor cells have been widely investigated for regenerative medicine and deemed promising in clinical applications. In order to further improve MSC-based stem cell therapeutics, it is important to understand the cellular kinetics and functional roles of MSCs in the dynamic regenerative processes. However, due to the heterogeneous nature of typical MSC cultures, their native identity and anatomical localization in the body have remained unclear, making it difficult to decipher the existence of distinct cell subsets within the MSC entity. Recent studies have shown that several blood-vessel-derived precursor cell populations, purified by flow cytometry from multiple human organs, give rise to bona fide MSCs, suggesting that the vasculature serves as a systemic reservoir of MSC-like stem/progenitor cells. Using individually purified MSC-like precursor cell subsets, we and other researchers have been able to investigate the differential phenotypes and regenerative capacities of these contributing cellular constituents in the MSC pool. In this review, we will discuss the identification and characterization of perivascular MSC precursors, including pericytes and adventitial cells, and focus on their cellular kinetics: cell adhesion, migration, engraftment, homing, and intercellular cross-talk during tissue repair and regeneration.

  16. Marketing: MSc in logistics and transportation

    OpenAIRE

    Jenkins, Mark

    1983-01-01

    The objective of this course is to introduce the basic concepts of marketing to students undertaking an MSc in Transportation and Logistics. This module will take a strategic view of marketing in that it will develop ideas for achieving sustainable competitive advantage.

  17. MSC POOL技术及其发展现状%MSC POOL Technology and Its Development Status

    Institute of Scientific and Technical Information of China (English)

    刘蓉; 李旭

    2011-01-01

    Based on technical research of MSC POOL, the definition and principle of MSC POOL technology are elaborated in a detail. The development status of various manufacturers for MSC POOL technology are summarized. Some typical business examples with MSC POOL technology and the benefits brought by MSC POOL technology in the practical application are analyzed. Finallyt some problems brought by MSC POOL technology and development trend of MSC POOL technology are proposed.%在对MSC POOL技术调研的基础上,详细阐述了MSC POOL技术的定义及原理,然后总结各个厂家针对MSCPOOL技术的发展状况,分析各地的一些典型商用MSC POOL技术实例以及MSC POOL技术在实际应用中所带来的优势,并总结了MSC POOL技术带来的一些问题,展望MSCPOOL技术未来的发展趋势.

  18. Mesenchymal stem cell 1 (MSC1-based therapy attenuates tumor growth whereas MSC2-treatment promotes tumor growth and metastasis.

    Directory of Open Access Journals (Sweden)

    Ruth S Waterman

    Full Text Available BACKGROUND: Currently, there are many promising clinical trials using mesenchymal stem cells (MSCs in cell-based therapies of numerous diseases. Increasingly, however, there is a concern over the use of MSCs because they home to tumors and can support tumor growth and metastasis. For instance, we established that MSCs in the ovarian tumor microenvironment promoted tumor growth and favored angiogenesis. In parallel studies, we also developed a new approach to induce the conventional mixed pool of MSCs into two uniform but distinct phenotypes we termed MSC1 and MSC2. METHODOLOGY/PRINCIPAL FINDINGS: Here we tested the in vitro and in vivo stability of MSC1 and MSC2 phenotypes as well as their effects on tumor growth and spread. In vitro co-culture of MSC1 with various cancer cells diminished growth in colony forming units and tumor spheroid assays, while conventional MSCs or MSC2 co-culture had the opposite effect in these assays. Co-culture of MSC1 and cancer cells also distinctly affected their migration and invasion potential when compared to MSCs or MSC2 treated samples. The expression of bioactive molecules also differed dramatically among these samples. MSC1-based treatment of established tumors in an immune competent model attenuated tumor growth and metastasis in contrast to MSCs- and MSC2-treated animals in which tumor growth and spread was increased. Also, in contrast to these groups, MSC1-therapy led to less ascites accumulation, increased CD45+leukocytes, decreased collagen deposition, and mast cell degranulation. CONCLUSION/SIGNIFICANCE: These observations indicate that the MSC1 and MSC2 phenotypes may be convenient tools for the discovery of critical components of the tumor stroma. The continued investigation of these cells may help ensure that cell based-therapy is used safely and effectively in human disease.

  19. MSC POOL网络组网方案研究

    Institute of Scientific and Technical Information of China (English)

    孙志刚; 姜金池

    2015-01-01

    MSC POOL是在3G R5版本中引入软交换核心网的一项重要技术。通过将某一区域的交换机组建成MSC POOL网络,极大地提高了通信网络的安全性。本文通过对MSC POOL技术进行深入研究,提出了一种以MGW媒体网关做NNSF代理的MSC POOL组网方式,并对可行性进行了论证。

  20. Student performance in a newly developed MSc programme

    DEFF Research Database (Denmark)

    Richelsen, Ann Bettina

    2011-01-01

    other than DTUs and allow qualified students to enter the MSc programmes. The focus of the present work is a comparison of how international and Danish students perform within specific modules of the MSc curriculum in Engineering Design and Applied Mechanics at Technical University of Denmark. The...

  1. MSc Thesis: Presentation of Certain New Trends in Noncommutative Geometry

    CERN Document Server

    Buachalla, Réamonn Ó

    2011-01-01

    MSc thesis of the author offering an introduction to the operator algebraic approach to noncommutative geometry, with a treatment of some more advanced elements such as the noncommutative geometry of quantum groups, fuzzy physics, and compact quantum metric spaces.

  2. Binding of fullerenes and nanotubes to MscL

    Science.gov (United States)

    Hilder, Tamsyn A.; Ridone, Pietro; Nakayama, Yoshitaka; Martinac, Boris; Chung, Shin-Ho

    2014-07-01

    Multi-drug resistance is becoming an increasing problem in the treatment of bacterial infections and diseases. The mechanosensitive channel of large conductance (MscL) is highly conserved among prokaryotes. Evidence suggests that a pharmacological agent that can affect the gating of, or block the current through, MscL has significant potential as a new class of antimicrobial compound capable of targeting a range of pathogenic bacteria with minimal side-effects to infected patients. Using molecular dynamics we examine the binding of fullerenes and nanotubes to MscL and demonstrate that both are stable within the MscL pore. We predict that fullerenes will attenuate the flow of ions through MscL by reducing the pore volume available to water and ions, but nanotubes will prevent pore closure resulting in a permanently open pore. Moreover, we confirm experimentally that it is possible to attenuate the flow of ions through MscL using a C60-γ cyclodextrin complex.

  3. NNSF Deployment within MSC Pool Technology%MSC Pool技术中NNSF的部署方式

    Institute of Scientific and Technical Information of China (English)

    龙滔滔

    2008-01-01

    本文首先介绍了MSC Pool的技术原理与组网优势,然后对MSC Pool技术中NNSF的两种部署方式做了重点阐述,说明了由MGW代理实现NNSF的信令组网方式,并且介绍了一种比较可行的组网实现方案.

  4. A new mesenchymal stem cell (MSC paradigm: polarization into a pro-inflammatory MSC1 or an Immunosuppressive MSC2 phenotype.

    Directory of Open Access Journals (Sweden)

    Ruth S Waterman

    Full Text Available BACKGROUND: Our laboratory and others reported that the stimulation of specific Toll-like receptors (TLRs affects the immune modulating responses of human multipotent mesenchymal stromal cells (hMSCs. Toll-like receptors recognize "danger" signals, and their activation leads to profound cellular and systemic responses that mobilize innate and adaptive host immune cells. The danger signals that trigger TLRs are released following most tissue pathologies. Since danger signals recruit immune cells to sites of injury, we reasoned that hMSCs might be recruited in a similar way. Indeed, we found that hMSCs express several TLRs (e.g., TLR3 and TLR4, and that their migration, invasion, and secretion of immune modulating factors is drastically affected by specific TLR-agonist engagement. In particular, we noted diverse consequences on the hMSCs following stimulation of TLR3 when compared to TLR4 by our low-level, short-term TLR-priming protocol. PRINCIPAL FINDINGS: Here we extend our studies on the effect on immune modulation by specific TLR-priming of hMSCs, and based on our findings, propose a new paradigm for hMSCs that takes its cue from the monocyte literature. Specifically, that hMSCs can be polarized by downstream TLR signaling into two homogenously acting phenotypes we classify here as MSC1 and MSC2. This concept came from our observations that TLR4-primed hMSCs, or MSC1, mostly elaborate pro-inflammatory mediators, while TLR3-primed hMSCs, or MSC2, express mostly immunosuppressive ones. Additionally, allogeneic co-cultures of TLR-primed MSCs with peripheral blood mononuclear cells (PBMCs predictably lead to suppressed T-lymphocyte activation following MSC2 co-culture, and permissive T-lymphocyte activation in co-culture with MSC1. SIGNIFICANCE: Our study provides an explanation to some of the conflicting reports on the net effect of TLR stimulation and its downstream consequences on the immune modulating properties of stem cells. We further

  5. Adaptation of MSC/NASTRAN to a supercomputer

    Energy Technology Data Exchange (ETDEWEB)

    Gloudeman, J.F.; Hodge, J.C.

    1982-01-01

    MSC/NASTRAN is a large-scale general purpose digital computer program which solves a wider variety of engineering analysis problems by the finite element method. The program capabilities include static and dynamic structural analysis (linear and nonlinear), heat transfer, acoustics, electromagnetism and other types of field problems. It is used worldwide by large and small companies in such diverse fields as automotive, aerospace, civil engineering, shipbuilding, offshore oil, industrial equipment, chemical engineering, biomedical research, optics and government research. The paper presents the significant aspects of the adaptation of MSC/NASTRAN to the Cray-1. First, the general architecture and predominant functional use of MSC/NASTRAN are discussed to help explain the imperatives and the challenges of this undertaking. The key characteristics of the Cray-1 which influenced the decision to undertake this effort are then reviewed to help identify performance targets. An overview of the MSC/NASTRAN adaptation effort is then given to help define the scope of the project. Finally, some measures of MSC/NASTRAN's operational performance on the Cray-1 are given, along with a few guidelines to help avoid improper interpretation. 17 references.

  6. YidC is required for the assembly of the MscL homopentameric pore

    NARCIS (Netherlands)

    Pop, Ovidiu I.; Soprova, Zora; Koningstein, Gregory; Scheffers, Dirk-Jan; Ulsen, Peter van; Wickström, David; Gier, Jan-Willem de; Luirink, Joen

    2009-01-01

    The mechanosensitive channel with large conductance (MscL) of Escherichia coli is formed by a homopentameric assembly of MscL proteins. Here, we describe MscL biogenesis as determined using in vivo approaches. Evidence is presented that MscL is targeted to the inner membrane via the signal recogniti

  7. MSC POOL工程实施方案研究

    Institute of Scientific and Technical Information of China (English)

    龙滔滔

    2012-01-01

    文章首先介绍MSC POOL技术的原理及优点,然后详细分析了在新建或改造MSC POOL过程中需要注意的问题,包括:池区规划的原则、需要规划的数据、四种POOL内组网方案的比较以及信令组网方式的选择,并总结了NRI与TMSI及系统容量的关系,为整个MSC POOL网络的工程实施提供了经验依据.

  8. MSC.Patran中的单位制转换%Units' Transition in Software of MSC.Patran

    Institute of Scientific and Technical Information of China (English)

    袁晓金; 段志梅

    2011-01-01

    以CAE为基础的虚拟产品开发(VPD)是实现创新设计的最主要技术保障.目前,在国际上被公认的最优秀的、应用最广泛的前后处理软件首推MSC公司的Patran软件.MSC.Patran是一个集成的并行框架式有限元前后处理及分析仿真系统.进行有限元分析计算必然涉及到单位制的转换问题,否则会因单位制产生错误.介绍了运用MSC.Patran软件时,正确进行单位制的转换的方法.%VPD on the basis of CAE is the best important technology guarantee of actual innovation designs. Now, MSC. Patran is recognized as the optimal and broad software of fore-and-aft disposal in the intemational. MSC. Patran is the compositive and side-by-side frame type system,which is used in the fore-and-aft disposal, analysis and simulation. There for units' transition is necessary in the FEA,otherwise,the system goes wrong. This paper introduces the means of unit system transition,handling MSC Patran software.

  9. MSC Pool组网规划研究及问题分析

    Institute of Scientific and Technical Information of China (English)

    黄嘉

    2007-01-01

    简要介绍了MSC Pool的技术原理,对目前MSC Pool技术应用存在的问题进行了分析,并针对引入MSC Pool带来的影响进行了探讨,重点讨论了 MSC Pool组网的要点和原则.并给出了MSC Pool组网规划的相关建议.

  10. TLR4 plays a crucial role in MSC-induced inhibition of NK cell function

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Ying [No. 307 Hospital of the Chinese People' s Liberation Army, Beijing (China); Liu, Jin; Liu, Yang; Qin, Yaru [Beijing Institute of Radiation Medicine, Beijing (China); Luo, Qun [No. 307 Hospital of the Chinese People' s Liberation Army, Beijing (China); Wang, Quanli, E-mail: 13691110351@163.com [No. 307 Hospital of the Chinese People' s Liberation Army, Beijing (China); Duan, Haifeng, E-mail: duanhf0720@163.com [Beijing Institute of Radiation Medicine, Beijing (China)

    2015-08-21

    Mesenchymal stem cells (MSC) are a kind of stromal cell within the tumor microenvironment. In our research, MSC derived from acute myeloid leukemia patients' bone marrow (AML-MSC) and lung cancer tissues (LC-MSC) as well as normal bone marrow-derived MSC (BM-MSC) cultured in conditioned medium of HeLa cells were found to have higher expressions of Toll-like receptor (TLR4) mRNA compared with BM-MSC. The sorted TLR4-positive MSC (TLR4+ MSC) differed in cytokine (interleukin-6, interleukin-8, and monocyte chemoattractant protein-1) secretion from those of unsorted MSC. MSC was reported to inhibit natural killer (NK) cell proliferation and function. In this research, we confirmed that TLR4+ MSC aggravate this suppression. Furthermore, when TLR4 in the sorted cells were stimulated by LPS or following blocked by antibody, the suppression on NK cell proliferation and cytotoxicity were more intensive or recovered respectively. Compared to unsorted MSC, NKG2D receptor expression on NK cells were also inhibited by TLR4+ MSC. These findings suggest that activation of TLR4 pathway is important for TLR4+ MSC and MSC to obstruct anti-tumor immunity by inhibiting NK cell function, which may provide a potential stroma-targeted tumor therapy. - Highlights: • TLR4+ MSC inhibit NK cell proliferation in vivo and in vitro. • TLR4+ MSC inhibit NKG2D expression on NK cells and NK cell cytotoxicity. • The distinguished cytokine expression of TLR4+ MSC may contribute to the inhibition on NK cell function.

  11. TLR4 plays a crucial role in MSC-induced inhibition of NK cell function

    International Nuclear Information System (INIS)

    Mesenchymal stem cells (MSC) are a kind of stromal cell within the tumor microenvironment. In our research, MSC derived from acute myeloid leukemia patients' bone marrow (AML-MSC) and lung cancer tissues (LC-MSC) as well as normal bone marrow-derived MSC (BM-MSC) cultured in conditioned medium of HeLa cells were found to have higher expressions of Toll-like receptor (TLR4) mRNA compared with BM-MSC. The sorted TLR4-positive MSC (TLR4+ MSC) differed in cytokine (interleukin-6, interleukin-8, and monocyte chemoattractant protein-1) secretion from those of unsorted MSC. MSC was reported to inhibit natural killer (NK) cell proliferation and function. In this research, we confirmed that TLR4+ MSC aggravate this suppression. Furthermore, when TLR4 in the sorted cells were stimulated by LPS or following blocked by antibody, the suppression on NK cell proliferation and cytotoxicity were more intensive or recovered respectively. Compared to unsorted MSC, NKG2D receptor expression on NK cells were also inhibited by TLR4+ MSC. These findings suggest that activation of TLR4 pathway is important for TLR4+ MSC and MSC to obstruct anti-tumor immunity by inhibiting NK cell function, which may provide a potential stroma-targeted tumor therapy. - Highlights: • TLR4+ MSC inhibit NK cell proliferation in vivo and in vitro. • TLR4+ MSC inhibit NKG2D expression on NK cells and NK cell cytotoxicity. • The distinguished cytokine expression of TLR4+ MSC may contribute to the inhibition on NK cell function

  12. M.Sc. in Civil and Structural Engineering

    DEFF Research Database (Denmark)

    The following pages contain a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short master projects as well as regular 3rd...... semester projects at the M.Sc. programme in Civil and Structural Engineering....

  13. Fit for purpose? Evaluation of an MSc. in Medical Physics.

    LENUS (Irish Health Repository)

    van der Putten, W J

    2014-05-01

    The National University of Ireland in Galway established a Master in Science (MSc.) program in medical physics in 2002. The course was designed to be 90 ECTS(1) credits and of one calendar year duration. From the outset the MSc. was designed to be part of an overall medical physics training program. MSc. programs are now widely used as part of the training and education of medical physicists. There is however paucity of data on the effectiveness of such courses and the purpose of the study reported here is to provide information on one particular MSc. course in medical physics. This is relevant to medical physicists who are involved in the development and running of medical physics training programs. The study used as methodology the Kirkpatrick levels of professional training. It was conducted through an online survey, both from students who graduated from the course and from students who were in the process of completing the course. The survey proved to be an effective way to determine attributes of modules such as learning outcomes, knowledge imparted, quality of teaching materials and others. The survey proved to be remarkably able to demonstrate interventions in the individual course modules. Although the course was shown to be effective in the imparting of the knowledge required to become a qualified medical physicist several areas for improvement were identified. These are mainly in the areas of increased practical experience and in course delivery.

  14. M.Sc. in Civil and Structural Engineering

    DEFF Research Database (Denmark)

    This catalogue contains a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short candidate projects as well as regular 3rd...... semester projects at the M.Sc. programme in Civil and Structural Engineering....

  15. M.Sc. in Civil and Structural Engineering

    DEFF Research Database (Denmark)

    The catalogue contain a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short candidate projects as well as regular 3rd semester...... projects at the M.Sc. programme in Civil and Structural Engineering....

  16. M.Sc. in Civil and Structural Engineering

    DEFF Research Database (Denmark)

    Clausen, Johan

    The report contain a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short candidate projects as well as regular 3rd semester...... projects at the M.Sc. programme in Civil and Structural Engineering....

  17. MSc degree in color technology for the automotive sector

    Science.gov (United States)

    Martinez-Verdu, F.; Perales, E.; Chorro, E.; Viqueira, V.; Gilabert, E.

    2014-07-01

    Nowadays, the measurement and management of color quality of the gonio-apparent materials is complex, but highly demanded in many industrial sectors, as automotive, cosmetics, plastics for consumer electronics, printing inks, architectural coatings, etc. It is necessary to control complex instrumentation and to do visual assessments of texture and color differences to get, for instance, a visual harmony in car bodies; and a profound knowledge of physics and chemistry of special-effect pigments for their optical formulation to obtain attractive visual effects in coatings, plastics, etc, combining among them and with solid pigments. From University of Alicante, for the academic year 2013-14, we are organizing the first MSc degree in Color Technology for the Automotive Sector, with a design of contents embracing CIE colorimetry and visual perception, included the AUDI2000 color difference formula, instrumentation and color management software, fundamentals of coatings and plastics in the automotive sector, and, optical formulation of pigments. The MSc syllabus, with 60 ECTS, is designed to be taught in two semesters: from September to February with on classroom theoretical and practical activities, and, from March to June at virtual level, with internships of training in some companies. Therefore, the MSc Thesis would be the performance report during the internship in companies or research institutions. Some multinational companies, both as car makers and coatings and plastics providers, from European and non-European countries have already shown their support and interest in welcoming students for specific training, even some job offers when the first MSc edition finishes.

  18. The Application of MSC POOL Technology in Mobile Communication%MSC POOL技术在移动通信中的应用

    Institute of Scientific and Technical Information of China (English)

    昂松鹤

    2014-01-01

    该文通过对MSC Pool技术介绍与优势的分析,阐明MSC Pool在软交换组网中的应用的重要性,分析了MSC POOL技术的关键点以及未来发展趋势,说明了MSC pool的实现方式、优缺点等问题。并对未来网络的展望。%This paper clarifies the importance of the use of MSC Pool in softswitch network through the introduction of MSC Pool and the analysis of its advantage. The paper also analyses the key points of MSC POOL and predicts its trend, which de-scribes its realization methods as well as its benefits and limits. At the end of this paper it looks ahead to the future of network.

  19. Expansion and Harvesting of hMSC-TERT

    DEFF Research Database (Denmark)

    Weber, Christian; Pohl, Sebastian; Pörtner, Ralf;

    2007-01-01

    cultivation and harvesting. Nonporous microcarriers are preferable when the cells need to be kept in viable condition for further applications like tissue engineering or cell therapy. In this study, the qualification of Biosilon, Cytodex 1, Cytodex 3, RapidCell and P102-L for expansion of h......MSC-TERT with an associated harvesting process using either trypsin, accutase, collagenase or a trypsin-accutase mixture was investigated. A subsequent adipogenic differentiation of harvested hMSC-TERT was performed in order to observe possible negative effects on their (adipogenic) differentiation potential as a result...... of the cultivation and harvesting method. The cultivated cells showed an average growth rate of 0.52 d(-1). The cells cultivated on Biosilon, RapidCell and P102-L were harvested succesfully achieving high cell yield and vitalities near 100%. This was not the case for cells on Cytodex 1 and Cytodex 3. The trypsin...

  20. MSc Agriculture students working with ex-campus stakeholders

    DEFF Research Database (Denmark)

    Langer, Vibeke; Lund, Mogens; Bendevis, Mira Arpe

    2014-01-01

    both on BSc and MSc level. For many students this results in concerns whether their knowledge, skills and competencies are sufficient when confronted with reality in a job, i.e. in a lack of professional confidence. Therefore when revising the program we focused on competences in contextualizing......In the MSc program in Agriculture at University of Copenhagen we experience that both domestic and international students increasingly enter the programme without a contextual background of “agriculture” and with solid, but fragmented disciplinary and applied knowledge acquired in other courses...... in collaboration with stakeholders. Students are physically on the partner enterprise four times, altogether three weeks, and use Kolb’s learning cycle as a guiding reference through a structured work process. Deliverables from the course are a scientific group report, a group partner document, a group learning...

  1. Differentiation of hMSC in Micromass Culture

    Institute of Scientific and Technical Information of China (English)

    Natalia; De; ISIA; Céline; HUSELSTEIN; Luc; MARCHAL; Marie-Nathalie; SARDA-KOLOPP; Jing-Ping; OU; YANG; Jean-Franois; STOLTZ; Assia; ELJAAFARI

    2005-01-01

    1 Introduction Mesenchymal stem cells (MSCs) are multipotential stem cells which can be expanded in culture while still maintaining their undifferentiated state. They have the potential to differentiate into distinct mesenchymal tissue cells, including chondrocytes. Thus, they are an attractive cell source for cartilage tissue engineering. In vitro high density micromass culture has been widely used for chondrogenesis induction. The objective of our study was to analyze viability and differentiation of hMSC...

  2. Using PAFEC as a preprocessor for MSC/NASTRAN

    Energy Technology Data Exchange (ETDEWEB)

    Gray, W.H.; Baudry, T.V.

    1983-01-01

    Programs for Automatic Finite Element Calculations (PAFEC) is a general-purpose, three-dimensional, linear and nonlinear finite element program. PAFEC's features include free-format input using engineering keywords, powerful mesh-generating facilities, sophisticated database management procedures, and extensive data validation checks. Presented here is a description of a software interface that permits PAFEC to be used as a preprocessor for MSC/NASTRAN. This user-friendly software, called PAFMSC, frees the stress analyst from the laborious and error-prone procedure of creating and debugging a rigid-format MSC/NASTRAN bulk data deck. By interactively creating and debugging a finite element model with PAFEC, thus taking full advantage of the free-format, engineering-keyword-oriented data structure of PAFEC, the stress analyst can drastically reduce the amount of time spent during model generation. The PAFMSC software will automatically convert a PAFEC data structure into an MSC/NASTRAN bulk data deck. The capabilities and limitations of the PAFMSC software are fully discussed in the following report.

  3. Lactococcus lactis Uses MscL as Its Principal Mechanosensitive Channel

    NARCIS (Netherlands)

    Folgering, Joost H.A.; Moe, Paul C.; Schuurman-Wolters, Gea K.; Blount, Paul; Poolman, Bert

    2005-01-01

    The functions of the mechanosensitive channels from Lactococcus lactis were determined by biochemical, physiological, and electrophysiological methods. Patchclamp studies showed that the genes yncB and mscL encode MscS and MscL-like channels, respectively, when expressed in Escherichia coli or if th

  4. Application of MSC POOL Technology in the Mobile Softswitch Network%MSC POOL技术在移动软交换组网中的应用

    Institute of Scientific and Technical Information of China (English)

    韩东红

    2014-01-01

    The application of MSC Pool in mobile softswitch network is very important. Its technology is the future development trend of softswitch. This article illustrates the application of MSC POOL and its advantages and disadvantages.%MSC POOL在移动软交换组网应用中非常重要,它的技术是未来软交换发展的趋势。本文说明MSC POOL的应用及优缺点等。

  5. Mesenchymal Stem Cell (MSC) Aggregate Formation in vivo

    Science.gov (United States)

    Bartosh, Thomas J.; Ylostalo, Joni H.

    2016-01-01

    Human mesenchymal stem/progenitor cells (MSCs) isolated from various adult tissues show remarkable therapeutic potential and are being employed in clinical trials for the treatment of numerous diseases (Prockop et al., 2010). While routes of cell administration vary, profound beneficial effects of MSCs in animal models have been observed following intraperitoneal injections of the cells (Roddy et al., 2011). Similar to MSC spheres formed in culture under conditions where attachment to plastic is not permitted (Bartosh et al., 2010), MSCs injected into the peritoneum of mice spontaneously aggregate into 3D sphere-like structures (Bartosh et al., 2013). During the process of sphere assembly and compaction, MSCs upregulate expression of numerous therapeutic anti-inflammatory and immune modulatory factors. Here we describe the method we previously used for the generation of human bone marrow-derived MSC aggregates/spheres in vivo (Bartosh et al., 2013). By tagging the MSCs with green fluorescent protein (GFP), the aggregates formed can be easily visualized, collected and analyzed for changes in cellular properties and interactions with host immune cells.

  6. Sensitivity and optimization of composite structures using MSC/NASTRAN

    Science.gov (United States)

    Nagendra, Gopal K.; Fleury, Claude

    1987-01-01

    Design sensitivity analysis for composites will soon be available in MSC/NASTRAN. The design variables for composites can be lamina thicknesses, orientation angles, material properties or a combination of all three. With the increasing use of composites in aerospace and automotive industries, this general capability can be used in its own right for carrying out sensitivity analysis of complicated real-life structures. As part of a research effort, the sensitivity analysis was coupled with a general purpose optimizer. This preliminary version of the optimizer is capable of dealing with minimum weight structural design with a rather general design variable linking capability at the element level or system level. Only sizing type of design variables (i.e., lamina thicknesses) can be handled by the optimizer. Test cases were run and validated by comparison with independent finite element packages. The linking of design sensitivity capability for composites in MSC/NASTRAN with an optimizer would give designers a powerful automated tool to carry out practical opitmization design of real-life complicated composite structures.

  7. 基于MSC POOL实现SERVER容灾的方案研究

    Institute of Scientific and Technical Information of China (English)

    徐萍

    2014-01-01

    本文简要介绍了MSC POOL的演进背景、MSC POOL的概念、容灾,并以新疆移动为例,着重从容灾实现方案的组网、数据准备、实施验证等三方面介绍了基于MSC POOL实现SERVER容灾的方案研究。

  8. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.

    Directory of Open Access Journals (Sweden)

    Natalie Fekete

    Full Text Available BACKGROUND: Mesenchymal stromal cells (MSC have gained importance in tissue repair, tissue engineering and in immunosupressive therapy during the last years. Due to the limited availability of MSC in the bone marrow, ex vivo amplification prior to clinical application is requisite to obtain therapeutic applicable cell doses. Translation of preclinical into clinical-grade large-scale MSC expansion necessitates precise definition and standardization of all procedural parameters including cell seeding density, culture medium and cultivation devices. While xenogeneic additives such as fetal calf serum are still widely used for cell culture, its use in the clinical context is associated with many risks, such as prion and viral transmission or adverse immunological reactions against xenogeneic components. METHODS AND FINDINGS: We established animal-free expansion protocols using platelet lysate as medium supplement and thereby could confirm its safety and feasibility for large-scale MSC isolation and expansion. Five different GMP-compliant standardized protocols designed for the safe, reliable, efficient and economical isolation and expansion of MSC was performed and MSC obtained were analyzed for differentiation capacity by qPCR and histochemistry. Expression of standard MSC markers as defined by the International Society for Cellular Therapy as well as expression of additional MSC markers and of various chemokine and cytokine receptors was analysed by flow cytometry. Changes of metabolic markers and cytokines in the medium were addressed using the LUMINEX platform. CONCLUSIONS: The five different systems for isolation and expansion of MSC described in this study are all suitable to produce at least 100 millions of MSC, which is commonly regarded as a single clinical dose. Final products are equal according to the minimal criteria for MSC defined by the ISCT. We showed that chemokine and integrin receptors analyzed had the same expression pattern

  9. Research on Load Transfer Method and Application of MSC Pool%MSC Pool负荷迁移机制及应用研究

    Institute of Scientific and Technical Information of China (English)

    黄华生

    2011-01-01

    针对常规MSC Pool负荷迁移后存在的MSC Pool中各MSC间负荷仍不均衡的情况,创立了基于话务模型的数据预测和多次迭代算法的迁移流程,实现了MSC Pool内的负荷均衡,并通过网管程序实现了负荷不均衡自动监测和负荷自动调整.

  10. M.Sc. in Civil and Structural Engineering

    DEFF Research Database (Denmark)

    Clausen, Johan Christian

    The following pages contain a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short master projects as well as regular 3rd...... semester projects at the M.Sc. programme in Civil and Structural Engineering. Each project description provides a brief overview of the purpose as well as the main activities. Further, a weighting between theoretical analysis, experimental work and computer modelling has been proposed. Usually...... page. Furthermore, other ideas for projects may be discussed with a potential supervisor. Many private engineering companies have a homepage on which they state that they would like to collaborate with students on a master project....

  11. The ‘devils triangle’ of MSC certification: Balancing credibility, accessibility and continuous improvement

    NARCIS (Netherlands)

    Bush, S.R.; Toonen, H.M.; Oosterveer, P.J.M.; Mol, A.P.J.

    2013-01-01

    The Marine Stewardship Council (MSC) has continued to strengthen its position in the market based on its credibility as a transparent, accountable and science-based third party certification scheme. However, the consolidation of MSC's credibility risks being undermined by the poor representation of

  12. Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC.

    Science.gov (United States)

    Adamzyk, Carina; Emonds, Tanja; Falkenstein, Julia; Tolba, René; Jahnen-Dechent, Wilhelm; Lethaus, Bernd; Neuss, Sabine

    2013-01-01

    Orthopedic implants including engineered bone tissue are commonly tested in sheep. To avoid rejection of heterologous or xenogeneic cells, autologous cells are preferably used, that is, ovine mesenchymal stem cells (oMSC). Unlike human MSC, ovine MSC are not well studied regarding isolation, expansion, and characterization. Here we investigated the impact of culture media composition on growth characteristics, differentiation, and surface antigen expression of oMSC. The culture media varied in fetal calf serum (FCS) content and in the addition of supplements and/or additional epidermal growth factor (EGF). We found that FCS strongly influenced oMSC proliferation and that specific combinations of supplemental factors (MCDB-201, ITS-plus, dexamethasone, and L-ascorbic acid) determined the expression of surface epitopes. We compared two published protocols for oMSC differentiation towards the osteogenic, adipogenic, and chondrogenic fate and found (i) considerable donor to donor variations, (ii) protocol-dependent variations, and (iii) variations resulting from the preculture medium composition. Our results indicate that the isolation and culture of oMSC in different growth media are highly variable regarding oMSC phenotype and behaviour. Furthermore, variations from donor to donor critically influence growth rate, surface marker expression, and differentiation.

  13. 基于lu_flex的MSC Pool技术分析

    Institute of Scientific and Technical Information of China (English)

    杨波; 樊自甫; 万晓榆

    2008-01-01

    MSC池(MSCin Pool)是由3GPP规范定义的,能优化整个移动网络的组网。本文简要介绍了MSC Pool技术的基本原理及其组网特点,分析了MSC Pool所用到的关键技术(NRI路由机制和负荷分担),重点讨论了核心网D接口信令的流量,根据MSC Pool的技术特点,提出了组网规划的前提条件和规划时需要注意的问题,最后总结了MSC Pool技术的特点,在结论部分给出了MSC Pool可能存在的安全隐患。

  14. Sympathetic denervation-induced MSC mobilization in distraction osteogenesis associates with inhibition of MSC migration and osteogenesis by norepinephrine/adrb3.

    Directory of Open Access Journals (Sweden)

    Zhaojie Du

    Full Text Available The sympathetic nervous system regulates bone formation and resorption under physiological conditions. However, it is still unclear how the sympathetic nerves affect stem cell migration and differentiation in bone regeneration. Distraction osteogenesis is an ideal model of bone regeneration due to its special nature as a self-engineering tissue. In this study, a rat model of mandibular distraction osteogenesis with transection of cervical sympathetic trunk was used to demonstrate that sympathetic denervation can deplete norepinephrine (NE in distraction-induced bone callus, down-regulate β3-adrenergic receptor (adrb3 in bone marrow mesenchymal stem cells (MSCs, and promote MSC migration from perivascular regions to bone-forming units. An in vitro Transwell assay was here used to demonstrate that NE can inhibit stroma-derived factor-1 (SDF-1-induced MSC migration and expression of the migration-related gene matrix metalloproteinase-2 (MMP-2 and downregulate that of the anti-migration gene tissue inhibitor of metalloproteinase-3 (TIMP-3. Knockdown of adrb3 using siRNA abolishes inhibition of MSC migration. An in vitro osteogenic assay was used to show that NE can inhibit the formation of MSC bone nodules and expression of the osteogenic marker genes alkaline phosphatase (ALP, osteocalcin (OCN, and runt-related transcription factor-2 (RUNX2, but knockdown of adrb3 by siRNA can abolish such inhibition of the osteogenic differentiation of MSCs. It is here concluded that sympathetic denervation-induced MSC mobilization in rat mandibular distraction osteogenesis is associated with inhibition of MSC migration and osteogenic differentiation by NE/adrb3 in vitro. These findings may facilitate understanding of the relationship of MSC mobilization and sympathetic nervous system across a wide spectrum of tissue regeneration processes.

  15. Sympathetic denervation-induced MSC mobilization in distraction osteogenesis associates with inhibition of MSC migration and osteogenesis by norepinephrine/adrb3.

    Science.gov (United States)

    Du, Zhaojie; Wang, Lei; Zhao, Yinghua; Cao, Jian; Wang, Tao; Liu, Peng; Zhang, Yabo; Yang, Xinjie; Cheng, Xiaobing; Liu, Baolin; Lei, Delin

    2014-01-01

    The sympathetic nervous system regulates bone formation and resorption under physiological conditions. However, it is still unclear how the sympathetic nerves affect stem cell migration and differentiation in bone regeneration. Distraction osteogenesis is an ideal model of bone regeneration due to its special nature as a self-engineering tissue. In this study, a rat model of mandibular distraction osteogenesis with transection of cervical sympathetic trunk was used to demonstrate that sympathetic denervation can deplete norepinephrine (NE) in distraction-induced bone callus, down-regulate β3-adrenergic receptor (adrb3) in bone marrow mesenchymal stem cells (MSCs), and promote MSC migration from perivascular regions to bone-forming units. An in vitro Transwell assay was here used to demonstrate that NE can inhibit stroma-derived factor-1 (SDF-1)-induced MSC migration and expression of the migration-related gene matrix metalloproteinase-2 (MMP-2) and downregulate that of the anti-migration gene tissue inhibitor of metalloproteinase-3 (TIMP-3). Knockdown of adrb3 using siRNA abolishes inhibition of MSC migration. An in vitro osteogenic assay was used to show that NE can inhibit the formation of MSC bone nodules and expression of the osteogenic marker genes alkaline phosphatase (ALP), osteocalcin (OCN), and runt-related transcription factor-2 (RUNX2), but knockdown of adrb3 by siRNA can abolish such inhibition of the osteogenic differentiation of MSCs. It is here concluded that sympathetic denervation-induced MSC mobilization in rat mandibular distraction osteogenesis is associated with inhibition of MSC migration and osteogenic differentiation by NE/adrb3 in vitro. These findings may facilitate understanding of the relationship of MSC mobilization and sympathetic nervous system across a wide spectrum of tissue regeneration processes. PMID:25144690

  16. LTE网络部署CSFB语音时MSC POOL解决方案研究%Research on MSC POOL Solution to CSFB Voice Deployed in LTE Network

    Institute of Scientific and Technical Information of China (English)

    张红霞

    2014-01-01

    LTE部署初期,运营商通过CSFB技术为LTE用户提供语音业务。现网2G/3G端局采用MSC POOL组网的情况下,部署CSFB功能后引入了一些新问题。基于此,通过调整组网和配置、MSC和MME流程优化等方法来解决,既完成了CSFB功能又体现了MSC POOL组网的优势。%In the early stage of LTE deployment, telecom operator provides voice service to LTE subscribers using CSFB(Circuit Switched Fallback) technology. However, with MSC POOL already applied in current 2G/3G networks, some new problems arise when CSFB technology is introduced. In view of this situations, they are can be solved by adjusting networking and configuration, as well as optimizing MSC and MME flows. Thus, the advantages of both CSFB function and MSC POOL networking are simultaneously implemented.

  17. The c-Met Inhibitor MSC2156119J Effectively Inhibits Tumor Growth in Liver Cancer Models

    Energy Technology Data Exchange (ETDEWEB)

    Bladt, Friedhelm, E-mail: Friedhelm.Bladt@merckgroup.com; Friese-Hamim, Manja; Ihling, Christian; Wilm, Claudia; Blaukat, Andree [EMD Serono, and Merck Serono Research and Development, Merck KGaA, Darmstadt 64293 (Germany)

    2014-08-19

    The mesenchymal-epithelial transition factor (c-Met) is a receptor tyrosine kinase with hepatocyte growth factor (HGF) as its only high-affinity ligand. Aberrant activation of c-Met is associated with many human malignancies, including hepatocellular carcinoma (HCC). We investigated the in vivo antitumor and antimetastatic efficacy of the c-Met inhibitor MSC2156119J (EMD 1214063) in patient-derived tumor explants. BALB/c nude mice were inoculated with MHCC97H cells or with tumor fragments of 10 patient-derived primary liver cancer explants selected according to c-Met/HGF expression levels. MSC2156119J (10, 30, and 100 mg/kg) and sorafenib (50 mg/kg) were administered orally as single-agent treatment or in combination, with vehicle as control. Tumor response, metastases formation, and alpha fetoprotein (AFP) levels were measured. MSC2156119J inhibited tumor growth and induced complete regression in mice bearing subcutaneous and orthotopic MHCC97H tumors. AFP levels were undetectable after 5 weeks of MSC2156119J treatment, and the number of metastatic lung foci was reduced. Primary liver explant models with strong c-Met/HGF activation showed increased responsiveness to MSC2156119J, with MSC2156119J showing similar or superior activity to sorafenib. Tumors characterized by low c-Met expression were less sensitive to MSC2156119J. MSC2156119J was better tolerated than sorafenib, and combination therapy did not improve efficacy. These findings indicate that selective c-Met/HGF inhibition with MSC2156119J is associated with marked regression of c-Met high-expressing tumors, supporting its clinical development as an antitumor treatment for HCC patients with active c-Met signaling.

  18. 包头联通核心网MSC POOL规划案例

    Institute of Scientific and Technical Information of China (English)

    宇文广

    2014-01-01

    通过对MSC POOL原理简要介绍,根据MSC POOL的实施目标及原则,以包头联通网络为例,将包头联通的网络现状作为基础,阐述CS域中MSS设备组POOL的具体实施方案.根据该方案的具体描述可以指导包头联通MSC POOL的建设.

  19. USING OF MSC WITH DIFFERENT ONTOGENETIC MATURITY FOR CORRECTION OF CHRONIC FIBROSING LIVER DAMAGE

    Directory of Open Access Journals (Sweden)

    M. Y. Shagidulin

    2013-01-01

    Full Text Available Aim. To compare the effectiveness of MSC with different degree of ontogenetic maturity (MSC bone marrow – MSC BM and MSC umbilical cord – MSC UC on regenerative processes in injured liver. Methods. In 4 groups of experiments on Wistar rats (n = 80 with a model of fibrotic toxic liver damage (FLD it was studied the effect of MSCs with different degree of ontogenetic maturity on recovery processes at the regeneration of damaged liver: 1 gr. – Control, 2 gr. and 3 gr. introduction of MSC BM, included in Sphero®GEL-long in doses of 2.5 ×106 and 5.0 x 106 cells, respectively, and 4 gr. – introduction of MSC UC in the form of cell-spheroids (8–10 × 105 cells. The cells were injected into the damaged liver in 7 days after the end of FDL-modeling. The effect of cell therapy was studied during 180 days. The effectiveness of corrective therapy was evaluated by the results of functional and morphological investigations of livers (histological control of parenchymal and nonparenchy- mal liver tissue. Results. MSC BM in both doses and MSC UC contributed to a more rapid normalization of liver enzyme indices compared with the control (1 gr., but the differences in the rate of recovery of disturbed enzymatic liver functions between groups 2, 3 and 4 – were absent. In 90 days after the cell application it was determined a more pronounced recovery activity of cells in groups 3 and 4; in 180 days the more pronounced activation of recovery processes was observed in group 3; but in group 4 the sclerotic processes were more pro- nounced in this period. Conclusion. For the induction of recovery processes in damage liver it is advisable not to use the MSC UC, but to use MSC BM in the Sphero®GEL, because MSC BM exert not only local but also systemic immune-regulatory effect, increasing the pool of T-reg. cells, which are additional carriers of regenera- tion information in organism. 

  20. Curriculum (Study Programme) for the M.Sc., Medialogy, at Aalborg University in Copenhagen

    DEFF Research Database (Denmark)

    Serafin, Stefania; Nordahl, Rolf; Lavatino, Salvatore

    2004-01-01

    Defining and describing the education, M.Sc., Medialogy. The curriculum (Study Programme) describes semesters, themes, projectunits, courses and contents of the eduation. offered at Aalborg University in Copenhagen....

  1. Defending the future: An MSc module in End User Computing Risk Management

    OpenAIRE

    Thorne, Simon

    2010-01-01

    This paper describes the rationale, curriculum and subject matter of a new MSc module being taught on an MSc Finance and Information Management course at the University of Wales Institute in Cardiff. Academic research on spreadsheet risks now has some penetration in academic literature and there is a growing body of knowledge on the subjects of spreadsheet error, human factors, spreadsheet engineering, "best practice", spreadsheet risk management and various techniques used to mitigate spread...

  2. Chronic exposure of low dose salinomycin inhibits MSC migration capability in vitro

    OpenAIRE

    Scherzad, Agmal; HACKENBERG, STEPHAN; FROELICH, KATRIN; RAK, KRISTEN; HAGEN, RUDOLF; TAEGER, JOHANNES; BREGENZER, MAXIMILLIAN; KLEINSASSER, NORBERT

    2016-01-01

    Salinomycin is a polyether antiprotozoal antibiotic that is used as a food additive, particularly in poultry farming. By consuming animal products, there may be a chronic human exposure to salinomycin. Salinomycin inhibits the differentiation of preadipocytes into adipocytes. As human mesenchymal stem cells (MSC) may differentiate into different mesenchymal cells, it thus appeared worthwhile to investigate whether chronic salinomycin exposure impairs the functional properties of MSC and induc...

  3. Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2

    Science.gov (United States)

    Caires, Hugo R.; Gomez-Lazaro, Maria; Oliveira, Carla M.; Gomes, David; Mateus, Denisa D.; Oliveira, Carla; Barrias, Cristina C.; Barbosa, Mário A.; Almeida, Catarina R.

    2015-05-01

    Mesenchymal Stem/Stromal Cells (MSC) are a promising cell type for cell-based therapies - from tissue regeneration to treatment of autoimmune diseases - due to their capacity to migrate to damaged tissues, to differentiate in different lineages and to their immunomodulatory and paracrine properties. Here, a simple and reliable imaging technique was developed to study MSC dynamical behavior in natural and bioengineered 3D matrices. Human MSC were transfected to express a fluorescent photoswitchable protein, Dendra2, which was used to highlight and follow the same group of cells for more than seven days, even if removed from the microscope to the incubator. This strategy provided reliable tracking in 3D microenvironments with different properties, including the hydrogels Matrigel and alginate as well as chitosan porous scaffolds. Comparison of cells mobility within matrices with tuned physicochemical properties revealed that MSC embedded in Matrigel migrated 64% more with 5.2 mg protein/mL than with 9.6 mg/mL and that MSC embedded in RGD-alginate migrated 51% faster with 1% polymer concentration than in 2% RGD-alginate. This platform thus provides a straightforward approach to characterize MSC dynamics in 3D and has applications in the field of stem cell biology and for the development of biomaterials for tissue regeneration.

  4. Interdisciplinary MSc and Doctoral Education in Climate System Science at the University of Hamburg

    Science.gov (United States)

    Dilly, Oliver; Stammer, Detlef; Pfeiffer, Eva-Maria

    2010-05-01

    Modern education in climate system sciences is based on a number of disciplines such as meteorology, geophysics, oceanography, geosciences and also economics and social sciences. Facts across these disciplines are required to address the faced key issues related to climate change effectively. Climate experts need to have profound knowledge that can only be achieved in interdisciplinary MSc and PhD programs. In Europe, graduate students who completed a BSc degree are typically proceeding with MSc programs to increase knowledge and qualification. Afterwards, the participation in a doctoral program may follow. Many doctoral programs include courses supporting disciplinary methodological and scientific background in particular. Those courses derive either from advanced MSc programs or specific trainings. Typically, interdisciplinary exchange is difficult to achieve at any stage of disciplinary graduate programs. Recent developments showed the need to educate climate experts in interdisciplinary MSc programs in climate system sciences for both researchers and professionals outside the university. The University of Hamburg offers an interdisciplinary 2-yr MSc program in Integrated System Sciences with 120 ECTS (30 compulsory, 90 eligible) in English language. If the MSc student decides to proceed with a PhD thesis, he/she may not necessarily complete the MSc program but may start to work on a specific and disciplinary doctoral thesis for 3 years. Each doctoral student is guided by an advisory panel (AP) which meets at least bi-annually. The AP consists of a Principal Advisor, a Co-Advisor and a Chair of the panel who come from neighboring disciplines. The structured doctoral program with only 12 CPs includes interdisciplinary compulsory courses and tailor-made eligible expert courses. Summer schools and soft skill courses add to both MSc and doctoral programs. Accordingly, the new graduate school concepts in climate system sciences at the University of Hamburg supports

  5. Mesenchymal stem cells (MSC prevented the progression of renovascular hypertension, improved renal function and architecture.

    Directory of Open Access Journals (Sweden)

    Elizabeth B Oliveira-Sales

    Full Text Available Renovascular hypertension induced by 2 Kidney-1 Clip (2K-1C is a renin-angiotensin-system (RAS-dependent model, leading to renal vascular rarefaction and renal failure. RAS inhibitors are not able to reduce arterial pressure (AP and/or preserve the renal function, and thus, alternative therapies are needed. Three weeks after left renal artery occlusion, fluorescently tagged mesenchymal stem cells (MSC (2×10(5 cells/animal were injected weekly into the tail vein in 2K-1C hypertensive rats. Flow cytometry showed labeled MSC in the cortex and medulla of the clipped kidney. MSC prevented a further increase in the AP, significantly reduced proteinuria and decreased sympathetic hyperactivity in 2K-1C rats. Renal function parameters were unchanged, except for an increase in urinary volume observed in 2K-1C rats, which was not corrected by MSC. The treatment improved the morphology and decreased the fibrotic areas in the clipped kidney and also significantly reduced renal vascular rarefaction typical of 2K-1C model. Expression levels of IL-1β, TNF-α angiotensinogen, ACE, and Ang II receptor AT1 were elevated, whereas AT2 levels were decreased in the medulla of the clipped kidney. MSC normalized these expression levels. In conclusion, MSC therapy in the 2K-1C model (i prevented the progressive increase of AP, (ii improved renal morphology and microvascular rarefaction, (iii reduced fibrosis, proteinuria and inflammatory cytokines, (iv suppressed the intrarenal RAS, iv decreased sympathetic hyperactivity in anesthetized animals and v MSC were detected at the CNS suggesting that the cells crossed the blood-brain barrier. This therapy may be a promising strategy to treat renovascular hypertension and its renal consequences in the near future.

  6. Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities

    Science.gov (United States)

    Sukharev, S. I.; Blount, P.; Martinac, B.; Kung, C.

    1997-01-01

    Although mechanosensory responses are ubiquitous and diverse, the molecular bases of mechanosensation in most cases remain mysterious MscL, a mechanosensitive channel of large conductance of Escherichia coli and its bacterial homologues are the first and currently only channel molecules shown to directly sense mechanical stretch of the membrane. In response to the tension conveyed via the lipid bilayer, MscL increases its open probability by several orders of magnitude. In the present review we describe the identification, cloning, and first sets of biophysical and structural data on this simplest mechanosensory molecule. We discovered a 2.5-ns mechanosensitive conductance in giant E. coli spheroplasts. Using chromatographies to enrich the target and patch clamp to assay the channel activity in liposome-reconstituted fractions, we identified the MscL protein and cloned the mscL gene. MscL comprises 136 amino acid residues (15 kDa), with two highly hydrophobic regions, and resides in the inner membrane of the bacterium. PhoA-fusion experiments indicate that the protein spans the membrane twice with both termini in the cytoplasm. Spectroscopic techniques show that it is highly helical. Expression of MscL tandems and covalent cross-linking suggest that the active channel complex is a homo-hexamer. We have identified several residues, which when deleted or substituted, affect channel kinetics or mechanosensitivity. Although unique when discovered, highly conserved MscL homologues in both gram-negative and gram-positive bacteria have been found, suggesting their ubiquitous importance among bacteria.

  7. Msc1 acts through histone H2A.Z to promote chromosome stability in Schizosaccharomyces pombe.

    Science.gov (United States)

    Ahmed, Shakil; Dul, Barbara; Qiu, Xinxing; Walworth, Nancy C

    2007-11-01

    As a central component of the DNA damage checkpoint pathway, the conserved protein kinase Chk1 mediates cell cycle progression when DNA damage is generated. Msc1 was identified as a multicopy suppressor capable of facilitating survival in response to DNA damage of cells mutant for chk1. We demonstrate that loss of msc1 function results in an increased rate of chromosome loss and that an msc1 null allele exhibits genetic interactions with mutants in key kinetochore components. Multicopy expression of msc1 robustly suppresses a temperature-sensitive mutant (cnp1-1) in the centromere-specific histone H3 variant CENP-A, and localization of CENP-A to the centromere is compromised in msc1 null cells. We present several lines of evidence to suggest that Msc1 carries out its function through the histone H2A variant H2A.Z, encoded by pht1 in fission yeast. Like an msc1 mutant, a pht1 mutant also exhibits chromosome instability and genetic interactions with kinetochore mutants. Suppression of cnp1-1 by multicopy msc1 requires pht1. Likewise, suppression of the DNA damage sensitivity of a chk1 mutant by multicopy msc1 also requires pht1. We present the first genetic evidence that histone H2A.Z may participate in centromere function in fission yeast and propose that Msc1 acts through H2A.Z to promote chromosome stability and cell survival following DNA damage. PMID:17947424

  8. Improving the Design of a MscL-Based Triggered Nanovalve

    Directory of Open Access Journals (Sweden)

    Paul Blount

    2013-03-01

    Full Text Available The mechanosensitive channel of large conductance, MscL, has been proposed as a triggered nanovalve to be used in drug release and other nanodevices. It is a small homopentameric bacterial protein that has the largest gated pore known: greater than 30 Å. Large molecules, even small proteins can be released through MscL. Although MscL normally gates in response to membrane tension, early studies found that hydrophilic or charged residue substitutions near the constriction of the channel leads to pore opening. Researchers have successfully changed the modality of MscL to open to stimuli such as light by chemically modifying a single residue, G22, within the MscL pore. Here, by utilizing in vivo, liposome efflux, and patch clamp assays we compared modification of G22 with that of another neighboring residue, G26, and demonstrate that modifying G26 may be a better choice for triggered nanovalves used for triggered vesicular release of compounds.

  9. Defending the future: An MSc module in End User Computing Risk Management

    CERN Document Server

    Thorne, Simon

    2010-01-01

    This paper describes the rationale, curriculum and subject matter of a new MSc module being taught on an MSc Finance and Information Management course at the University of Wales Institute in Cardiff. Academic research on spreadsheet risks now has some penetration in academic literature and there is a growing body of knowledge on the subjects of spreadsheet error, human factors, spreadsheet engineering, "best practice", spreadsheet risk management and various techniques used to mitigate spreadsheet errors. This new MSc module in End User Computing Risk Management is an attempt to pull all of this research and practitioner experience together to arm the next generation of finance spreadsheet champions with the relevant knowledge, techniques and critical perspective on an emerging discipline.

  10. Msc1 Acts Through Histone H2A.Z to Promote Chromosome Stability in Schizosaccharomyces pombe

    OpenAIRE

    Ahmed, Shakil; Dul, Barbara; Qiu, Xinxing; Walworth, Nancy C.

    2007-01-01

    As a central component of the DNA damage checkpoint pathway, the conserved protein kinase Chk1 mediates cell cycle progression when DNA damage is generated. Msc1 was identified as a multicopy suppressor capable of facilitating survival in response to DNA damage of cells mutant for chk1. We demonstrate that loss of msc1 function results in an increased rate of chromosome loss and that an msc1 null allele exhibits genetic interactions with mutants in key kinetochore components. Multicopy expres...

  11. MSC POOL相邻池区切换自动均衡实现

    Institute of Scientific and Technical Information of China (English)

    陈鑫

    2014-01-01

    本文针对MSC POOL大规模组网部署后出现的相邻池区间跨POOL切换不均衡导致用户分布不均匀、话务不均衡问题,介绍了如何实现跨POOL切换的自动均衡、可控、可调,对MSC POOL多池区组网规划优化有一定参考应用价值。

  12. ANALYSIS WITH MSC ADAMS OF A 5-FINGER AND 3-PHALANX /FINGER UNDER-ACTUATEDMECHANICAL HAND

    Directory of Open Access Journals (Sweden)

    Gheorghe POPESCU

    2013-05-01

    Full Text Available This paper studies the analysis with MSC ADAMS of a 5-fingered and 3-phalanx/finger underactuatedmechanical hand, designed by the author to work on industrial robots. Moreover, in order to increasegrasping safety in the automated handling process, the author has fitted each finger with a locking sequence inthe final phase of grasping. Thus, the mechanism of mechanical hand is considered to be a mechanical systemand is treated like a set of rigid bodies connected by mechanical linkages and elastic elements. To model andsimulate this mechanism with MSC ADAMS programme, the author covered the following stages: constructionof the model, testing-simulation, validation, finishing, parameterization, and optimization

  13. Reimplementing the Mathematical Subject Classification (MSC) as a Linked Open Dataset

    CERN Document Server

    Lange, Christoph; Dimou, Anastasia; Bratsas, Charalampos; Corneli, Joseph; Sperber, Wolfram; Kohlhase, Michael; Antoniou, Ioannis

    2012-01-01

    The Mathematics Subject Classification (MSC) is a widely used scheme for classifying documents in mathematics by subject. Its traditional, idiosyncratic conceptualization and representation makes the scheme hard to maintain and requires custom implementations of search, query and annotation support. This limits uptake e.g. in semantic web technologies in general and the creation and exploration of connections between mathematics and related domains (e.g. science) in particular. This paper presents the new official implementation of the MSC2010 as a Linked Open Dataset, building on SKOS (Simple Knowledge Organization System). We provide a brief overview of the dataset's structure, its available implementations, and first applications.

  14. Supervising M.Sc. Students working in the 100 Gigabit Ethernet field using OPNET Modeler

    DEFF Research Database (Denmark)

    Ruepp, Sarah Renée; Berger, Michael Stübert; Wessing, Henrik

    2010-01-01

    This paper deals with supervision methods for M.Sc. students who are using OPNET Modeler for their thesis work within the field of 100 Gigabit Ethernet. We detail how we use OPNET Modeler in our M.Sc. projects at the Technical University of Denmark. In particular, we discuss on how we teach...... students to learn OPNET independently and in a short timeframe, and we outline what students find challenging and rewarding by using OPNET Modeler. Furthermore, we show some cases on how OPNET was applied in specific projects within the field of 100 Gigabit Ethernet....

  15. Education of MSc and PhD Students in Fluid Power and Mechatronics at DTU

    DEFF Research Database (Denmark)

    Conrad, Finn

    1996-01-01

    The paper deals with education of MSc and PhD students in engineering areas fluid power and mechatronics at the Technical Univ of Denmark, DTU, Lyngby. The new education structure and programs for MSc and PhD students adapted to the change and development of technologies. Focus is on two of twenty...... engineering profilies:(1) Engineeing Design and Product Development and (2)Control Engineering which give possibilitie for specialisation in fluid power and mechatronics design and productdevelopment. Synthesis, design and self-learning competency have a high priority taking the importance of training...

  16. Further Developments on Optimum Structural Design Using MSC/Nastran and Sequential Quadratic Programming

    DEFF Research Database (Denmark)

    Holzleitner, Ludwig

    1996-01-01

    This work is closely connected to the paper: K.G. MAHMOUD, H.W. ENGL and HOLZLEITNER: "OPTIMUM STRUCTURAL DESIGN USING MSC/NASTRAN AND SEQUENTIAL QUADRATIC PROGRAMMING", Computers & Structures, Vol. 52, No. 3, pp. 437-447, (1994). In contrast to that paper, where thickness optimization is described...... analysis and gradient calculation are used, together with sequential quadratic programming with an active set strategy for optimization. The optimization and sensitivity analysis modules are efficiently coupled with MSC/NASTRAN using DMAP (direct matrix abstraction program) statements. To demonstrate...

  17. Therapeutic use of human mesenchymal stem cells (MSC) for the treatment of radio-induced diseases

    International Nuclear Information System (INIS)

    Ionising radiation can induce toxic effects on body. They provoke physiological modifications of tissues and organs which can be lethal. Total body irradiation or local abdominal irradiation can induce serious complications. Intestine is the first tissue concerned by these side effects. Radiation induces malabsorption of the intestine and lost of it integrity. Radio-induced physiopathological effects on intestine could lead to distant effects on other tissues and organs such as liver. The actual treatments have a limited efficiency or are not adapted to gastrointestinal damages. Indeed, in this type of lesions, the heterogeneous systems which are concerned and the gravity of lesions complicates the medical care. Our purpose is to show that cell therapy using human mesenchymal stem cells (MSC) constitutes resolution in this type of illness. The works which are presented in this thesis show that MSC are multi-potent and have heterogeneous expression of molecules. These cells are able to establish themselves in many organs and tissues after injection into irradiated body. Thus we have shown that MSC can prevent the small intestine from radio-induced damages. Indeed we demonstrate that through their actions on gut, MSC can indirectly restore hepatic integrity. (author)

  18. Studies on sensitivity to tension and gating pathway of MscL by molecular dynamic simulation

    Institute of Scientific and Technical Information of China (English)

    Jun-Yu Xie; Guang-Hong Ding

    2013-01-01

    Mechanosensitive (MS) ion channels play an important role in various physiological processes.Although the determination of the structure of mechanosensitive channel of large conductance (MscL) makes the simulation study possible,it has not so far been possible to directly simulate the gating mechanism of MscL in atomic detail.In this article,MscL has been studied via molecular dynamic (MD)simulations to gain a detailed description of the sensitivity to lateral tension and the gating pathway.MscL undergoes conformational rearrangement in sustaining lateral tension,and the open state is obtained when 2.0 MPa lateral tension is directly applied on the pure protein.During the opening process,Loop region responds to tension first,and the mechanical sensitivity is followed by S1 domain.Transmembrane (TM) bundle is the key position for channel opening,and the motion of TM1 helices finally realizes the significant expansion of the constricted gating pore.C-terminus domain presents expansion later during the TM opening.In our study,return of the whole protein to the initial closed state is achieved only in the early opening stage.During the relaxation from the open state,the TM helices are the most mobile domain,which is different from the opening process.

  19. Structural Investigation of MscL Gating Using Experimental Data and Coarse Grained MD Simulations

    NARCIS (Netherlands)

    Deplazes, Evelyne; Louhivuori, Martti; Jayatilaka, Dylan; Marrink, Siewert J.; Corry, Ben; Elofsson, Arne

    2012-01-01

    The mechanosensitive channel of large conductance (MscL) has become a model system in which to understand mechanosensation, a process involved in osmoregulation and many other physiological functions. While a high resolution closed state structure is available, details of the open structure and the

  20. Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore

    Science.gov (United States)

    Gao, Ya; Li, Hua; Wang, Junmei; Blount, Paul

    2016-01-01

    The primary mechanism of action of the antibiotic dihydrostreptomycin is binding to and modifying the function of the bacterial ribosome, thus leading to decreased and aberrant translation of proteins; however, the routes by which it enters the bacterial cell are largely unknown. The mechanosensitive channel of large conductance, MscL, is found in the vast majority of bacterial species, where it serves as an emergency release valve rescuing the cell from sudden decreases in external osmolarity. While it is known that MscL expression increases the potency of dihydrostreptomycin, it has remained unclear if this effect is due to a direct interaction. Here, we use a combination of genetic screening, MD simulations, and biochemical and mutational approaches to determine if dihydrostreptomycin directly interacts with MscL. Our data strongly suggest that dihydrostreptomycin binds to a specific site on MscL and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell. PMID:27280286

  1. New MSc programme in Sustainable Energy at Risø DTU

    DEFF Research Database (Denmark)

    Ryde, M.

    2007-01-01

    In September 2008, the fi rst batch of students will start a new MSc programme which is based at Risø DTU. It is a two-year study programme, so the students will be well into their studies when the UN’s climate conference is held in Copenhagen in 2009...

  2. Competencies of BSc and MSc programmes in electrical engineering and student portfolios

    NARCIS (Netherlands)

    Mouthaan, Ton J.; Brink, R.W.; Vos, H.

    2002-01-01

    General goals of a BSc and MSc in electrical engineering are formulated, leading to a 'mission' statement clarifying the rationale behind the programmes in relation to the needs of society. These goals are made operational in terms of competencies that engineers educated through our programmes are r

  3. Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy.

    Science.gov (United States)

    Lech, Wioletta; Figiel-Dabrowska, Anna; Sarnowska, Anna; Drela, Katarzyna; Obtulowicz, Patrycja; Noszczyk, Bartlomiej Henryk; Buzanska, Leonora; Domanska-Janik, Krystyna

    2016-01-01

    Mesenchymal stem cells (MSC) exhibit enormous heterogeneity which can modify their regenerative properties and therefore influence therapeutic effectiveness as well as safety of these cells transplantation. In addition the high phenotypic plasticity of MSC population makes it enormously sensitive to any changes in environmental properties including fluctuation in oxygen concentration. We have shown here that lowering oxygen level far below air atmosphere has a beneficial impact on various parameters characteristic for umbilical cord Wharton Jelly- (WJ-) MSC and adipose tissue- (AD-) derived MSC cultures. This includes their cellular composition, rate of proliferation, and maintenance of stemness properties together with commitment to cell differentiation toward mesodermal and neural lineages. In addition, the culture genomic stability increased significantly during long-term cell passaging and eventually protected cells against spontaneous transformation. Also by comparing of two routinely used methods of MSCs isolation (mechanical versus enzymatic) we have found substantial divergence arising between cell culture properties increasing along the time of cultivation in vitro. Thus, in this paper we highlight the urgent necessity to develop the more sensitive and selective methods for prediction and control cells fate and functioning during the time of growth in vitro. PMID:27651796

  4. Optimization and translation of MSC-based hyaluronic acid hydrogels for cartilage repair

    Science.gov (United States)

    Erickson, Isaac E.

    2011-12-01

    Traumatic injury and disease disrupt the ability of cartilage to carry joint stresses and, without an innate regenerative response, often lead to degenerative changes towards the premature development of osteoarthritis. Surgical interventions have yet to restore long-term mechanical function. Towards this end, tissue engineering has been explored for the de novo formation of engineered cartilage as a biologic approach to cartilage repair. Research utilizing autologous chondrocytes has been promising, but clinical limitations in their yield have motivated research into the potential of mesenchymal stem cells (MSCs) as an alternative cell source. MSCs are multipotent cells that can differentiate towards a chondrocyte phenotype in a number of biomaterials, but no combination has successfully recapitulated the native mechanical function of healthy articular cartilage. The broad objective of this thesis was to establish an MSC-based tissue engineering approach worthy of clinical translation. Hydrogels are a common class of biomaterial used for cartilage tissue engineering and our initial work demonstrated the potential of a photo-polymerizable hyaluronic acid (HA) hydrogel to promote MSC chondrogenesis and improved construct maturation by optimizing macromer and MSC seeding density. The beneficial effects of dynamic compressive loading, high MSC density, and continuous mixing (orbital shaker) resulted in equilibrium modulus values over 1 MPa, well in range of native tissue. While compressive properties are crucial, clinical translation also demands that constructs stably integrate within a defect. We utilized a push-out testing modality to assess the in vitro integration of HA constructs within artificial cartilage defects. We established the necessity for in vitro pre-maturation of constructs before repair to achieve greater integration strength and compressive properties in situ. Combining high MSC density and gentle mixing resulted in integration strength over 500 k

  5. Mechanical strain downregulates C/EBPβ in MSC and decreases endoplasmic reticulum stress.

    Directory of Open Access Journals (Sweden)

    Maya Styner

    Full Text Available Exercise prevents marrow mesenchymal stem cell (MSC adipogenesis, reversing trends that accompany aging and osteoporosis. Mechanical input, the in-vitro analogue to exercise, limits PPARγ expression and adipogenesis in MSC. We considered whether C/EBPβ might be mechanoresponsive as it is upstream to PPARγ, and also is known to upregulate endoplasmic reticulum (ER stress. MSC (C3H10T1/2 pluripotent cells as well as mouse marrow-derived MSC were cultured in adipogenic media and a daily mechanical strain regimen was applied. We demonstrate herein that mechanical strain represses C/EBPβ mRNA (0.6-fold ±0.07, p<0.05 and protein (0.4-fold ±0.1, p<0.01 in MSC. SiRNA silencing of β-catenin prevented mechanical repression of C/EBPβ. C/EBPβ overexpression did not override strain's inhibition of adipogenesis, which suggests that mechanical control of C/EBPβ is not the primary site at which adipogenesis is regulated. Mechanical inhibition of C/EBPβ, however, might be critical for further processes that regulate MSC health. Indeed, overexpression of C/EBPβ in MSC induced ER stress evidenced by a dose-dependent increase in the pro-apoptotic CHOP (protein 4-fold ±0.5, p<0.05 and a threshold reduction in the chaperone BiP (protein 0.6-fold ±0.1, p = 0.2; mRNA 0.3-fold ±0.1, p<0.01. ChIP-seq demonstrated a significant association between C/EBPβ and both CHOP and BiP genes. The strain regimen, in addition to decreasing C/EBPβ mRNA (0.5-fold ±0.09, p<0.05, expanded ER capacity as measured by an increase in BiP mRNA (2-fold ±0.2, p<0.05 and protein. Finally, ER stress induced by tunicamycin was ameliorated by mechanical strain as demonstrated by decreased C/EBPβ, increased BiP and decreased CHOP protein expression. Thus, C/EBPβ is a mechanically responsive transcription factor and its repression should counter increases in marrow fat as well as improve skeletal resistance to ER stress.

  6. Simulation of Air Suspension and Full-vehicle with MSC Adams/Car%基于MSC Adams/Car的空气悬架及整车仿真

    Institute of Scientific and Technical Information of China (English)

    王登峰; 郎锡泽; 马天飞

    2006-01-01

    利用MSC Adams/Car软件建立载货汽车后空气悬架系统多体动力学模型,仿真计算了悬架垂直刚度特性,证明模型的正确性. 建立Adams/Car的整车模型,进行稳态回转试验的仿真分析. 将仿真结果与道路试验结果进行比较,验证虚拟样机模型的正确性.

  7. Novel isolation strategy to deliver pure fetal-origin and maternal-origin mesenchymal stem cell (MSC) populations from human term placenta.

    Science.gov (United States)

    Patel, J; Shafiee, A; Wang, W; Fisk, N M; Khosrotehrani, K

    2014-11-01

    The placenta is an abundant source of mesenchymal stem/stromal cells (MSC). Although presumed of translationally-advantageous fetal origin, the literature instead suggests a high incidence of either contaminating or pure maternal MSC. Despite definitional criteria that MSC are CD34-, increasing evidence suggests that fetal MSC may be CD34 positive in vivo. We flow sorted term placental digests based on CD34+ expression and exploited differential culture media to isolate separately pure fetal and maternal MSC populations. This method has considerable translational implications, in particular to clinical trials underway with "placental" MSC of uncertain or decidual origin.

  8. Suicide gene reveals the myocardial neovascularization role of mesenchymal stem cells overexpressing CXCR4 (MSC(CXCR4.

    Directory of Open Access Journals (Sweden)

    Jialiang Liang

    Full Text Available BACKGROUND: Our previous studies indicated that MSC(CXCR4 improved cardiac function after myocardial infarction (MI. This study was aimed to investigate the specific role of MSC(CXCR4 in neovascularization of infarcted myocardium using a suicide gene approach. METHODS: MSCs were transduced with either lentivirus-null vector/GFP (MSC(Null as control or vector encoding for overexpressing CXCR4/GFP. The MSC derived-endothelial cell (EC differentiation was assessed by a tube formation assay, Dil-ac-LDL uptake, EC marker expression, and VE-cadherin promoter activity assay. Gene expression was analyzed by quantitative RT-PCR or Western blot. The suicide gene approach was under the control of VE-cadherin promoter. In vivo studies: Cell patches containing MSC(Null or MSC(CXCR4 were transduced with suicide gene and implanted into the myocardium of MI rat. Rats received either ganciclovir (GCV or vehicle after cell implantation. After one month, the cardiac functional changes and neovascularization were assessed by echocardiography, histological analysis, and micro-CT imaging. RESULTS: The expression of VEGF-A and HIF-1α was significantly higher in MSC(CXCR4 as compared to MSC(Null under hypoxia. Additionally, MSC(CXCR4 enhanced new vessel formation and EC differentiation, as well as STAT3 phosphorylation under hypoxia. STAT3 participated in the transcription of VE-cadherin in MSC(CXCR4 under hypoxia, which was inhibited by WP1066 (a STAT3 inhibitor. In addition, GCV specifically induced death of ECs with suicide gene activation. In vivo studies: MSC(CXCR4 implantation promoted cardiac functional restoration, reduced infarct size, improved cardiac remodeling, and enhanced neovascularization in ischemic heart tissue. New vessels derived from MSC(CXCR4 were observed at the injured heart margins and communicated with native coronary arteries. However, the derived vessel networks were reduced by GCV, reversing improvement of cardiac function. CONCLUSION: The

  9. MSc Dissertation

    DEFF Research Database (Denmark)

    Roued-Cunliffe, Henriette

    2008-01-01

    Heritage Portals are in this dissertation defined as deep portals which can give access to external resource contents. This dissertation has developed such a portal which accesses the Swedish SMR, FMIS and the ARK system developed by L – P : Archaeology through web services. The development...... of the web Service for the ARK system was done as a part of this project combined with the creating of a WFS web-mapping service serving the spatial part of the dataset collected as a part of the Sintana project. The textual part of this dataset has been incorporated into an ARK system in the same way...... that the Portus project dataset was. Both these projects are available through the ARK web service. The portal accessed the FMIS and ARK web service and re-maps the XML output to Midas standard formatted XML and combined them. This allows the portal to do a cross-search of the two datasets and return the data...

  10. The Application of MSC Pool Technology in Soft Switch Equipment Disaster Recovery%MSC Pool技术在软交换设备容灾中的应用

    Institute of Scientific and Technical Information of China (English)

    刘扬

    2008-01-01

    重点对MSC Pool技术进行了介绍,并对该技术应用在软交换设备容灾时的优势和可能出现的问题进行了分析,提出了在现网中软交换设备采用MSC Pool组网问题的解决思路并对MSC Pool方式组网建设的应用前景进行了展望.

  11. The Marine Stewardship Council (MSC) and the Making of a Market for ‘Sustainable Fish’

    DEFF Research Database (Denmark)

    Ponte, Stefano

    2012-01-01

    Market-based instruments of fishery governance have been promoted in the past two decades on the basis of two widespread expectations: that complying with sustainability standards will lead to environmental benefits; and that certifications will not discriminate against specific social groups...... the market for ‘sustainable fish’, but success has been accompanied by serious challenges. The MSC has so far failed to convincingly show that its certification system has positive environmental impacts, and it has marginalized Southern fisheries, especially in low-income countries. As an institutional...... solution to the global fishery crisis, the MSC seems to be better tuned to the creation of a market for ‘sustainable fish’ rather than ‘sustainable fisheries’....

  12. Optimasi Penambahan Colcemid pada Karyotyping Kultur Mecenchymal Stem Cells (MSC Mencit

    Directory of Open Access Journals (Sweden)

    Ratih Rinendyaputri

    2016-02-01

    Full Text Available AbstractControl of the genetic stability of stem cells prior to the conduct of therapy is essential to prevent effects such as stem cell transformation. Karyotyping is a conventional technique to conduct an analysis of the number and structure of chromosomes. The analysis can only be performed on metaphase stage that needs to be optimized to get the cell at that stage because the length of the cell cycle are different in the each cell types. This study aims to obtain an optimal time to get MSC at metaphase stage. The study was conducted at the stem cell laboratory of Center for Biomedical and Basic Technology of Health. The event begins with isolation using flushing technique at the femur and tibia of mice. Furthermore, the culture in vitro and induction colcemid 0,25μg/ml for 8,16 and 24 hours to get the MSC at metaphase stage. KCl solution with a concentration of 0.075 M and 0,045 M used as a solvent hipotonis. Results showed that 16 hours of induction colcemid 0,25μg/ml in 0.075 M KCl solution usage percentage of MSC who are at metaphase stage and do the highest analysis (p<0.05. In this study 16 hours induction colcemid 0,25μg/ml is the optimal time to obtain metaphase stage of the MSC from bone marrow of mice.Keywords: mecenchymal stem cell, karyotyping, colcemidAbstrakKontrol terhadap stabilitas genetik pada sel punca sebelum pelaksanan terapi merupakan hal yang penting untuk mencegah efek seperti transformasi sel punca yang dapat terjadi. Secara konvensional dapat dilakukan karyotyping untuk melakukan analisis terhadap jumlah dan struktur kromosom. Analisis hanya dapat dilakukan pada tahap metafase sehingga perlu dilakukan optimasi untuk mendapatkan sel pada tahap tersebut mengingat panjang siklus sel setiap jenis sel berbeda. Penelitian ini bertujuan untuk memperoleh waktu yang optimal untuk mendapatkan MSC pada tahap metafase. Penelitian dilakukan di Laboratorium stem cell Pusat Biomedis dan Teknologi Dasar Kesehatan Badan Litbangkes

  13. The finite element analysis program MSC Marc/Mentat a first introduction

    CERN Document Server

    Öchsner, Andreas

    2016-01-01

    Based on simple examples, this book offers a short introduction to the general-purpose finite element program MSC Marc, a specialized program for non-linear problems (implicit solver) distributed by the MSC Software Corporation, which is commonly used in academia and industry. Today the documentation of all finite element programs includes a variety of step-by-step examples of differing complexity, and in addition, all software companies offer professional workshops on different topics. As such, rather than competing with these, the book focuses on providing simple examples, often single-element problems, which can easily be related to the theory that is discussed in finite element lectures. This makes it an ideal companion book to classical introductory courses on the finite element method.

  14. Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis.

    Directory of Open Access Journals (Sweden)

    Federica Viti

    Full Text Available The culture of progenitor mesenchymal stem cells (MSC onto osteoconductive materials to induce a proper osteogenic differentiation and mineralized matrix regeneration represents a promising and widely diffused experimental approach for tissue-engineering (TE applications in orthopaedics. Among modern biomaterials, calcium phosphates represent the best bone substitutes, due to their chemical features emulating the mineral phase of bone tissue. Although many studies on stem cells differentiation mechanisms have been performed involving calcium-based scaffolds, results often focus on highlighting production of in vitro bone matrix markers and in vivo tissue ingrowth, while information related to the biomolecular mechanisms involved in the early cellular calcium-mediated differentiation is not well elucidated yet. Genetic programs for osteogenesis have been just partially deciphered, and the description of the different molecules and pathways operative in these differentiations is far from complete, as well as the activity of calcium in this process. The present work aims to shed light on the involvement of extracellular calcium in MSC differentiation: a better understanding of the early stage osteogenic differentiation program of MSC seeded on calcium-based biomaterials is required in order to develop optimal strategies to promote osteogenesis through the use of new generation osteoconductive scaffolds. A wide spectrum of analysis has been performed on time-dependent series: gene expression profiles are obtained from samples (MSC seeded on calcium-based scaffolds, together with related microRNAs expression and in vivo functional validation. On this basis, and relying on literature knowledge, hypotheses are made on the biomolecular players activated by the biomaterial calcium-phosphate component. Interestingly, a key role of miR-138 was highlighted, whose inhibition markedly increases osteogenic differentiation in vitro and enhance ectopic bone

  15. Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis.

    Science.gov (United States)

    Viti, Federica; Landini, Martina; Mezzelani, Alessandra; Petecchia, Loredana; Milanesi, Luciano; Scaglione, Silvia

    2016-01-01

    The culture of progenitor mesenchymal stem cells (MSC) onto osteoconductive materials to induce a proper osteogenic differentiation and mineralized matrix regeneration represents a promising and widely diffused experimental approach for tissue-engineering (TE) applications in orthopaedics. Among modern biomaterials, calcium phosphates represent the best bone substitutes, due to their chemical features emulating the mineral phase of bone tissue. Although many studies on stem cells differentiation mechanisms have been performed involving calcium-based scaffolds, results often focus on highlighting production of in vitro bone matrix markers and in vivo tissue ingrowth, while information related to the biomolecular mechanisms involved in the early cellular calcium-mediated differentiation is not well elucidated yet. Genetic programs for osteogenesis have been just partially deciphered, and the description of the different molecules and pathways operative in these differentiations is far from complete, as well as the activity of calcium in this process. The present work aims to shed light on the involvement of extracellular calcium in MSC differentiation: a better understanding of the early stage osteogenic differentiation program of MSC seeded on calcium-based biomaterials is required in order to develop optimal strategies to promote osteogenesis through the use of new generation osteoconductive scaffolds. A wide spectrum of analysis has been performed on time-dependent series: gene expression profiles are obtained from samples (MSC seeded on calcium-based scaffolds), together with related microRNAs expression and in vivo functional validation. On this basis, and relying on literature knowledge, hypotheses are made on the biomolecular players activated by the biomaterial calcium-phosphate component. Interestingly, a key role of miR-138 was highlighted, whose inhibition markedly increases osteogenic differentiation in vitro and enhance ectopic bone formation in vivo

  16. Cell therapy medicinal product regulatory framework in Europe and its application for MSC based therapy development

    Directory of Open Access Journals (Sweden)

    Janis eAncans

    2012-08-01

    Full Text Available Advanced therapy medicinal products (ATMPs, including cell therapy products, form a new class of medicines in the European Union. Since ATMPs are at the forefront of scientific innovation in medicine, specific regulatory framework has been developed for these medicines and implemented from 2009. The Committee for Advanced Therapies (CAT has been established at European Medicines Agency (EMA for centralized classification, certification and evaluation procedures, and other ATMP related tasks. Guidance documents, initiatives and interaction platforms are available to make the new framework more accessible for small and medium-sized enterprises, academia, hospitals and foundations. Good understanding of centralised and national components of the regulatory system is required to plan product development. It is in the best interests of cell therapy developers to utilise provided resources starting with the preclinical stage. Whilst there have not been mesenchymal stem cell (MSC based medicine authorisations in the EU, three MSC products have received marketing approval in other regions since 2011. Information provided on regulatory requirements, procedures and initiatives is aimed to facilitate MSC based medicinal product development and authorisation in the EU.

  17. The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168.

    Science.gov (United States)

    Kouwen, Thijs R H M; Trip, Erik N; Denham, Emma L; Sibbald, Mark J J B; Dubois, Jean-Yves F; van Dijl, Jan Maarten

    2009-11-01

    Bacillus subtilis strain 168 produces the extremely stable and broad-spectrum lantibiotic sublancin 168. Known sublancin 168-susceptible organisms include important pathogens, such as Staphylococcus aureus. Nevertheless, since its discovery, the mode of action of sublancin 168 has remained elusive. The present studies were, therefore, aimed at the identification of cellular determinants for bacterial susceptibility toward sublancin 168. Growth inhibition and competition assays on plates and in liquid cultures revealed that sublancin 168-mediated growth inhibition of susceptible B. subtilis and S. aureus cells is affected by the NaCl concentration in the growth medium. Added NaCl did not influence the production, activity, or stability of sublancin 168 but, instead, lowered the susceptibility of sensitive cells toward this lantibiotic. Importantly, the susceptibility of B. subtilis and S. aureus cells toward sublancin 168 was shown to depend on the presence of the large mechanosensitive channel of conductance MscL. In contrast, MscL was not involved in susceptibility toward the bacteriocin nisin or Pep5. Taken together, our unprecedented results demonstrate that MscL is a critical and specific determinant in bacterial sublancin 168 susceptibility that may serve either as a direct target for this lantibiotic or as a gate of entry to the cytoplasm. PMID:19738010

  18. Finite element modeling of composite piezoelectric structures with MSC/NASTRAN

    Science.gov (United States)

    Freed, Brian D.; Babuska, Vit

    1997-06-01

    Techniques for modeling structures containing piezoelectric ceramics with MSC/NASTRAN are presented. Unlike other finite element programs such as ANSYS and ABAQUS, MSC/NASTRAN offers no piezoelectric coupled-field elements with which to model smart structures directly. Rather, the analogy between piezoelectric strain and thermally induced strain, which allows temperature change to model piezoelectric voltage actuation, must be used. The application and limitations of this method are discussed. To overcome some of the limitations in modeling piezoelectric effects with the thermal analogy, one and two dimensional finite elements which include piezoelectric coupling were developed and integrated into MSC/NASTRAN as dummy elements. The dummy elements offer an alternative method for modeling piezoelectric structural members. As actuators, the elements support charge and voltage actuation in both static and dynamic analyses. When used as sensors, both strain and strain rate outputs are supported. The elements can be used for modal, transient, and frequency response solutions and facilitate combined thermal and piezoelectric loading.

  19. Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering.

    Science.gov (United States)

    Steinmetz, Neven J; Aisenbrey, Elizabeth A; Westbrook, Kristofer K; Qi, H Jerry; Bryant, Stephanie J

    2015-07-01

    A bioinspired multi-layer hydrogel was developed for the encapsulation of human mesenchymal stem cells (hMSCs) as a platform for osteochondral tissue engineering. The spatial presentation of biochemical cues, via incorporation of extracellular matrix analogs, and mechanical cues, via both hydrogel crosslink density and externally applied mechanical loads, were characterized in each layer. A simple sequential photopolymerization method was employed to form stable poly(ethylene glycol)-based hydrogels with a soft cartilage-like layer of chondroitin sulfate and low RGD concentrations, a stiff bone-like layer with high RGD concentrations, and an intermediate interfacial layer. Under a compressive load, the variation in hydrogel stiffness within each layer produced high strains in the soft cartilage-like layer, low strains in the stiff bone-like layer, and moderate strains in the interfacial layer. When hMSC-laden hydrogels were cultured statically in osteochondral differentiation media, the local biochemical and matrix stiffness cues were not sufficient to spatially guide hMSC differentiation after 21 days. However dynamic mechanical stimulation led to differentially high expression of collagens with collagen II in the cartilage-like layer, collagen X in the interfacial layer and collagen I in the bone-like layer and mineral deposits localized to the bone layer. Overall, these findings point to external mechanical stimulation as a potent regulator of hMSC differentiation toward osteochondral cellular phenotypes.

  20. 探讨MSC Pool技术在移动软交换网络的研究和应用

    Institute of Scientific and Technical Information of China (English)

    丁中华

    2014-01-01

    MSC Pool技术在移动软交换网络中起到了十分重要的作用,本文对MSC Pool技术的概念和工作原理做了介绍,并分析了MSC Pool技术的要点,以及组建MSC Pool的前提条件。

  1. Research on MSC Pool Tecnology and the Solution of Called Recovery for Huawei Enterprise%MSC Pool技术与华为被叫恢复方案

    Institute of Scientific and Technical Information of China (English)

    马欢

    2010-01-01

    MSC Pool是3G核心网建设中最受关注的技术之一,其理论优势在移动现网中有充分表现.使用该技术可满足网络容灾的需要,解决移动网络中的"潮汐效应",减少局间切换及局问位置更新.华为被叫快速恢复方案采用SCCP信令点负荷分担方式实现对PRN信令的备份处理,可用于解决MSC容灾故障.

  2. MSC POOL Technology Risk Analysis of Migration Patterns of Different Users%MSC POOL技术中不同用户迁移方式风险分析

    Institute of Scientific and Technical Information of China (English)

    陈巍

    2011-01-01

    该文介绍了MSC POOL的解决方案与技术优势,然后针对MSC POOL中用户迁移的两种方案做了重点阐述,并对不同方案进行风险分析,分析出对不同组网方式下对现网用户的影响.

  3. Infusion of Trx-1-overexpressing hucMSC prolongs the survival of acutely irradiated NOD/SCID mice by decreasing excessive inflammatory injury.

    Directory of Open Access Journals (Sweden)

    JiangWei Hu

    Full Text Available A protective reagent for ARI should have the ability to repair injured tissue caused by radiation and prevent continuous damage from secondary risk factors. Trx-1 was explored as a candidate therapy for ARI, as it scavenges reactive oxygen species, regulates cell growth and differentiation, participates in immune reactions, and inhibits apoptosis by acting inside and/or outside cells. Trx-1 can also decrease excessive inflammation in ARI by regulating the creation of inflamed media, by inhibiting the activation of complement, and by reducing the chemotaxis, adhesion, and migration of inflammatory cells. As effectively and stably expressing exogenous genes in the long term and regulating immune inflammation and tissue repair, MSC are a good choice for Trx-1 gene therapy. In this study, Trx-1-overexpressing hucMSC-Trx-1 were obtained by adenoviral vector-mediated infection. We first measured the redox capacity of hucMSC-Trx-1 with an antioxidant capacity (T-AOC assay, a hydrogen peroxide (H2O2 content determination assay in vivo, a H2O2-induced oxidation hemolysis assay, and a lipid peroxidation assay in vitro. Then, we measured survival time, the protection of the hematopoietic system, and the regulation of inflammation in important organs in three treatment groups of NOD/SCID mice (treated with hucMSC-Trx-1, with hucMSC, and with saline that were exposed to 4.5 Gy (60Co-γ-ray radiation. The hucMSC-Trx-1 group achieved superior antioxidation results, protecting bone marrow hematopoietic stem cells (Lin(-CD117(+: hucMSC-Trx-1 vs. hucMSC, P<0.05; hucMSC-Trx-1 vs. NS, P<0.01, promoting the formation of red blood cells and hemoglobin (hucMSC-Trx-1 vs. hucMSC or NS, P<0.05, reducing inflammation and damage in important organs (Bone marrow and lung: hucMSC-Trx-1 vs. NS, P<0.01; hucMSC-Trx-1 vs. hucMSC, P<0.05. Liver and intestine: hucMSC-Trx-1 vs. NS, P<0.05; hucMSC-Trx-1 vs. hucMSC, P<0.05, and prolonging survival (hucMSC-Trx-1 vs. hucMSC or NS, P<0

  4. A Conversation with David Bates, MD, MSc, Chairman of the American Medical Informatics Association

    OpenAIRE

    Raymond, Brian

    2008-01-01

    A world-renowned physician-researcher, David Bates, MD, MSc, is Chief of the Division of General Medicine at the Brigham and Women's Hospital in Boston, MA, and a Professor at Harvard Medical School and the Harvard School of Public Health, where he is codirector of the Program in Clinical Effectiveness. He is the Medical Director of Clinical and Quality Analysis, Information Systems at Partners HealthCare System, Inc. He is also the former Chair of the National Alliance for Primary Care Infor...

  5. DYNAMIC ANALYSIS OF A CRIMPING DEVICE WITH MULTIPLE CAMS USING MSC ADAMS II

    Directory of Open Access Journals (Sweden)

    Gheorghe Popescu

    2012-05-01

    Full Text Available Through the present paper, the author presents the results of the dynamic analysis with MSC ADAMS of the mechanism with a crimping device with 12 tightening cams, designed and used in the technological process of assembly of the indigenous electrical detonators. In this sense, the mechanism with multiple cams is considered a mechanical system and is treated as an assembly of rigid bodies connected by mechanical connections and elastic elements. For shaping and simulation of the mechanism with multiple cams using ADAMS program, the author got through the following stages: construction of the pattern, its testing and simulation, validation, finishing, parametrization, optimization of the pattern.

  6. A Modal Analysis of the Violin Using MSC/NASTRAN and PATRAN

    OpenAIRE

    Knott, George Anthony

    1987-01-01

    The MSC/NASTRAN finite element computer program and a Cray XMP computer were used to study the modal characteristics of a violin with the Stradivari shape . The violin geometry was modeled using an arcs of circles scheme with PATRAN, a finite element graphics pre/postprocessor program. The violin was modeled in-vacu and with free boundry conditions. Belly, back, sound post, bassbar, neck, bridge, tail-piece, strings, rib linings, end and corner blocks are the components of the model. Mode sha...

  7. THE EFFECTS OF CULTURE ON KNOWLEDGE MANAGEMENT PRACTICE: A QUALITATIVE CASE STUDY OF MSC STATUS COMPANIES

    Directory of Open Access Journals (Sweden)

    Charmaine Ryan

    2006-01-01

    Full Text Available Knowledge is recognised as being an important asset in organisations these days. Despite this, many organisations are not doing enough to effectively manage this important asset for its competitive advantage. In response to this, knowledge management which is defined as a process that effectively creates, captures, shares and uses organisation-wide knowledge to improve the organisation’s performance was conceived and has since gained widespread acceptance the world over. Despite its widespread acceptance, little is known about the current levels of knowledge management within the Malaysian context, in particular amongst the Multimedia Super Corridor (MSC status companies in Malaysia. Furthermore, the extent to which cultural factors impact upon knowledge management practice in these companies is not known. This study investigated the various cultural factors (collaboration, mutual trust, leadership and incentives/rewards using a multiple case study approach operating within a critical realism research paradigm and found that these factors have impact on the level of knowledge management practice. The study also established that cultural factors do play an important role in facilitating knowledge management practice in these MSC status companies in Malaysia. It was found that collaboration, mutual trust, leadership, kiasu-ism and incentives/rewards have significant impact on the level of knowledge management practice. In view of the findings of this study, it is suggested that the relevant authorities pay adequate attention on these cultural factors to ensure that the knowledge management initiatives undertaken by Malaysian companies are effectively deployed.

  8. The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels.

    Science.gov (United States)

    Bavi, Navid; Cortes, D Marien; Cox, Charles D; Rohde, Paul R; Liu, Weihong; Deitmer, Joachim W; Bavi, Omid; Strop, Pavel; Hill, Adam P; Rees, Douglas; Corry, Ben; Perozo, Eduardo; Martinac, Boris

    2016-01-01

    The bacterial mechanosensitive channel MscL gates in response to membrane tension as a result of mechanical force transmitted directly to the channel from the lipid bilayer. MscL represents an excellent model system to study the basic biophysical principles of mechanosensory transduction. However, understanding of the essential structural components that transduce bilayer tension into channel gating remains incomplete. Here using multiple experimental and computational approaches, we demonstrate that the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane. We propose that this may also represent a common principle in the gating cycle of unrelated mechanosensitive ion channels, allowing the coupling of channel conformation to membrane dynamics. PMID:27329693

  9. Structural investigation of MscL gating using experimental data and coarse grained MD simulations.

    Directory of Open Access Journals (Sweden)

    Evelyne Deplazes

    Full Text Available The mechanosensitive channel of large conductance (MscL has become a model system in which to understand mechanosensation, a process involved in osmoregulation and many other physiological functions. While a high resolution closed state structure is available, details of the open structure and the gating mechanism remain unknown. In this study we combine coarse grained simulations with restraints from EPR and FRET experiments to study the structural changes involved in gating with much greater level of conformational sampling than has previously been possible. We generated a set of plausible open pore structures that agree well with existing open pore structures and gating models. Most interestingly, we found that membrane thinning induces a kink in the upper part of TM1 that causes an outward motion of the periplasmic loop away from the pore centre. This previously unobserved structural change might present a new mechanism of tension sensing and might be related to a functional role in osmoregulation.

  10. Cause of Errors Associated with Application of Drucker-Prager Yield Criterion in MSC/NASTRAN Program%MSC/NASTRAN程序中Drucker-Prager屈服准则引起误差的原因探讨

    Institute of Scientific and Technical Information of China (English)

    王进军

    2000-01-01

    When the Drucker-Prager yield criterion is used, the MSC/NASTRAN program frequently predicts noticeable errors. The relevant part of NASTRAN Handbook was checked and some errors in the elasto-plastics theory used in NASTRAN were detected in this paper. The procurement to prove these errors was verified by numerical calculation.

  11. Capacity Building in IWRM: The IWRM MSc Curriculum at the Water and Environment Centre, Republic of Yemen

    NARCIS (Netherlands)

    Soppe, R.W.O.; Babaqi, A.S.; Huibers, F.P.

    2005-01-01

    Integrated Water Resources Management (IWRM) is an interdisciplinary approach to water resources management, as opposed to water resources development. In developing an MSc curriculum at the Water and Environment Centre (WEC) at Sana'a University in the republic of Yemen, three goals were defined. T

  12. On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface.

    Science.gov (United States)

    Iscla, Irene; Wray, Robin; Blount, Paul

    2008-09-01

    The mechanosensitive channel of large conductance, MscL, serves as a biological emergency release valve protecting bacteria from acute osmotic downshock and is to date the best characterized mechanosensitive channel. A well-recognized and supported model for Escherichia coli MscL gating proposes that the N-terminal 11 amino acids of this protein form a bundle of amphipathic helices in the closed state that functionally serves as a cytoplasmic second gate. However, a recently reexamined crystal structure of a closed state of the Mycobacterium tuberculosis MscL shows these helices running along the cytoplasmic surface of the membrane. Thus, it is unclear if one structural model is correct or if they both reflect valid closed states. Here, we have systematically reevaluated this region utilizing cysteine-scanning, in vivo functional characterization, in vivo SCAM, electrophysiological studies, and disulfide-trapping experiments. The disulfide-trapping pattern and functional studies do not support the helical bundle and second-gate hypothesis but correlate well with the proposed structure for M. tuberculosis MscL. We propose a functional model that is consistent with the collective data. PMID:18515388

  13. On the role of individual subunits in MscL gating : "All for one, one for all?"

    NARCIS (Netherlands)

    Mika, Jacek T.; Birkner, Jan P.; Poolman, Bert; Kocer, Armagan

    2013-01-01

    The mechanosensitive channel of large conductance (MscL) is a homopentameric membrane protein that protects bacteria from hypoosmotic stress. Its mechanics are coupled to structural changes in the membrane, yet the molecular mechanism of the transition from closed to open states and the cooperation

  14. Läätsa Kalatööstus järjekordne MSC sertifikaadi omanik / Alar Mik

    Index Scriptorium Estoniae

    Mik, Alar

    2014-01-01

    MSC (Marine Stewardship Council)-märgis toodetel tõendab, et tegemist on keskkonnaalaselt vastutustundliku ettevõttega, kelle toodangutsükkel on kala püüdmisest kuni valmistoodangu tarbijani jõudmiseni rangelt seaduslik ja keskkonnasäästlik. Vestlusest ettevõtte juhatuse liikme Toomas Auliga

  15. M.Sc. in Civil and Structural Engineering:3rd Semester and Master’s Thesis Ideas 2014

    OpenAIRE

    2014-01-01

    The following pages contain a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short master projects as well as regular 3rd semester projects at the M.Sc. programme in Civil and Structural Engineering.

  16. M.Sc. in Civil and Structural Engineering:3rd Semester and Master’s Thesis Ideas 2011

    OpenAIRE

    2011-01-01

    This catalogue contains a list of project ideas proposed by the scientific staff at the Department of Civil Engineering, Aalborg University, and a number of companies. Most of the project ideas in this catalogue may form the basis for long and short candidate projects as well as regular 3rd semester projects at the M.Sc. programme in Civil and Structural Engineering.

  17. Combined MSC-Secreted Factors and Neural Stem Cell Transplantation Promote Functional Recovery of PD Rats.

    Science.gov (United States)

    Yao, Yuan; Huang, Chen; Gu, Ping; Wen, Tieqiao

    2016-01-01

    Stem cell transplantation has enormous potential for the treatment of neurodegenerative disorders like Parkinson's disease (PD). Mesenchymal stem cells (MSCs) have attracted much attention because they can secrete a wide variety of cellular factors that promote cell growth. In this study, we prepared a conditioned medium (CM) using lyophilized MSC culture medium that contained the secretome of MSCs and applied this CM to the culture of neural stem cells (CM-NSCs) for the transplantation of PD model rats. Quantitative real-time PCR, Western blot, and immunocytochemistry were used to identify cell differentiation and expression of dopaminergic neuron-specific genes in vitro. Behavioral tests including rotational behavior and MWM training tests were also performed to assess the recovery. Our results indicated that combined treatment of CM and neural stem cell transplantation can significantly reduce apomorphine-induced rotational asymmetry and improve spatial learning ability. The CM-NSCs were able to differentiate into dopaminergic neurons in the ventral tegmental area (VTA) and medial forebrain bundle (MFB), and migrated around the lesion site. They showed a higher activity than untreated NSCs in cell survival, migration, and behavior improvement in the dopa-deficit rat model. These findings suggest that the neural stem cells treated with conditioned medium possess a great potential as a graft candidate for the treatment of Parkinson's disease. PMID:26607204

  18. Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes.

    Science.gov (United States)

    Jossen, Valentin; Schirmer, Cedric; Mostafa Sindi, Dolman; Eibl, Regine; Kraume, Matthias; Pörtner, Ralf; Eibl, Dieter

    2016-01-01

    The potential of human mesenchymal stem cells (hMSCs) for allogeneic cell therapies has created a large amount of interest. However, this presupposes the availability of efficient scale-up procedures. Promising results have been reported for stirred bioreactors that operate with microcarriers. Recent publications focusing on microcarrier-based stirred bioreactors have demonstrated the successful use of Computational Fluid Dynamics (CFD) and suspension criteria (N S1u , N S1) for rapidly scaling up hMSC expansions from mL- to pilot scale. Nevertheless, one obstacle may be the formation of large microcarrier-cell-aggregates, which may result in mass transfer limitations and inhomogeneous distributions of stem cells in the culture broth. The dependence of microcarrier-cell-aggregate formation on impeller speed and shear stress levels was investigated for human adipose derived stromal/stem cells (hASCs) at the spinner scale by recording the Sauter mean diameter (d 32) versus time. Cultivation at the suspension criteria provided d 32 values between 0.2 and 0.7 mm, the highest cell densities (1.25 × 10(6) cells mL(-1) hASCs), and the highest expansion factors (117.0 ± 4.7 on day 7), while maintaining the expression of specific surface markers. Furthermore, suitability of the suspension criterion N S1u was investigated for scaling up microcarrier-based processes in wave-mixed bioreactors for the first time. PMID:26981131

  19. Therapeutic effects of hMAPC and hMSC transplantation after stroke in mice.

    Directory of Open Access Journals (Sweden)

    Silvia Mora-Lee

    Full Text Available Stroke represents an attractive target for stem cell therapy. Although different types of cells have been employed in animal models, a direct comparison between cell sources has not been performed. The aim of our study was to assess the effect of human multipotent adult progenitor cells (hMAPCs and human mesenchymal stem cells (hMSCs on endogenous neurogenesis, angiogenesis and inflammation following stroke. BALB/Ca-RAG 2(-/- γC(-/- mice subjected to FeCl(3 thrombosis mediated stroke were intracranially injected with 2 × 10(5 hMAPCs or hMSCs 2 days after stroke and followed for up to 28 days. We could not detect long-term engraftment of either cell population. However, in comparison with PBS-treated animals, hMSC and hMAPC grafted animals demonstrated significantly decreased loss of brain tissue. This was associated with increased angiogenesis, diminished inflammation and a glial-scar inhibitory effect. Moreover, enhanced proliferation of cells in the subventricular zone (SVZ and survival of newly generated neuroblasts was observed. Interestingly, these neuroprotective effects were more pronounced in the group of animals treated with hMAPCs in comparison with hMSCs. Our results establish cell therapy with hMAPCs and hMSCs as a promising strategy for the treatment of stroke.

  20. Analysis of SMA Hybrid Composite Structures in MSC.Nastran and ABAQUS

    Science.gov (United States)

    Turner, Travis L.; Patel, Hemant D.

    2005-01-01

    A thermoelastic constitutive model for shape memory alloy (SMA) actuators and SMA hybrid composite (SMAHC) structures was recently implemented in the commercial finite element codes MSC.Nastran and ABAQUS. The model may be easily implemented in any code that has the capability for analysis of laminated composite structures with temperature dependent material properties. The model is also relatively easy to use and requires input of only fundamental engineering properties. A brief description of the model is presented, followed by discussion of implementation and usage in the commercial codes. Results are presented from static and dynamic analysis of SMAHC beams of two types; a beam clamped at each end and a cantilever beam. Nonlinear static (post-buckling) and random response analyses are demonstrated for the first specimen. Static deflection (shape) control is demonstrated for the cantilever beam. Approaches for modeling SMAHC material systems with embedded SMA in ribbon and small round wire product forms are demonstrated and compared. The results from the commercial codes are compared to those from a research code as validation of the commercial implementations; excellent correlation is achieved in all cases.

  1. Targeting P38 Pathway Regulates Bony Formation via MSC Recruitment during Mandibular Distraction Osteogenesis in Rats

    Science.gov (United States)

    Yang, Zi-hui; Wu, Bao-lei; Ye, Chen; Jia, Sen; Yang, Xin-jie; Hou, Rui; Lei, De-lin; Wang, Lei

    2016-01-01

    Distraction osteogenesis (DO) is a widely used self-tissue engineering. However, complications and discomfort due to the long treatment period are still the bottleneck of DO. Novel strategies to accelerate bone formation in DO are still needed. P38 is capable of regulating the osteogenic differentiation of both mesenchymal stem cells (MSCs) and osteoblasts, which are crucial to bone regeneration. However, it is not clear whether targeting p38 could regulate bony formation in DO. The purpose of the current work was to investigate the effects of local application of either p38 agonist anisomycin or p38 inhibitor SB203580 in a rat model of DO. 30 adult rats were randomly divided into 3 groups: (A) rats injected with DMSO served as the control group; (B) rats injected with p38 agonist anisomycin; (C) rats injected with p38 inhibitor SB203580. All the rats were subjected to mandibular distraction and the injection was performed daily during this period. The distracted mandibles were harvested on days 15 and 30 after surgery and subjected to the following analysis. Micro-computed tomography and histological evaluation results showed that local application of p38 agonist anisomycin increased new bone formation in DO, whereas p38 inhibitor SB203580 decreased it. Immunohistochemical analysis suggested that anisomycin promoted MSC recruitment in the distraction gap. In conclusion, this study demonstrated that local application of p38 agonist anisomycin can increase new bone formation during DO. This study may lead to a novel cell-based strategy for the improvement of bone regeneration. PMID:27766028

  2. Mutations in a Conserved Domain of E. coli MscS to the Most Conserved Superfamily Residue Leads to Kinetic Changes.

    Directory of Open Access Journals (Sweden)

    Hannah R Malcolm

    Full Text Available In Escherichia coli (E. coli the mechanosensitive channel of small conductance, MscS, gates in response to membrane tension created from acute external hypoosmotic shock, thus rescuing the bacterium from cell lysis. E. coli MscS is the most well studied member of the MscS superfamily of channels, whose members are found throughout the bacterial and plant kingdoms. Homology to the pore lining helix and upper vestibule domain of E. coli MscS is required for inclusion into the superfamily. Although highly conserved, in the second half of the pore lining helix (TM3B, E. coli MscS has five residues significantly different from other members of the superfamily. In superfamilies such as this, it remains unclear why variations within such a homologous region occur: is it tolerance of alternate residues, or does it define functional variance within the superfamily? Point mutations (S114I/T, L118F, A120S, L123F, F127E/K/T and patch clamp electrophysiology were used to study the effect of changing these residues in E. coli MscS on sensitivity and gating. The data indicate that variation at these locations do not consistently lead to wildtype channel phenotypes, nor do they define large changes in mechanosensation, but often appear to effect changes in the E. coli MscS channel gating kinetics.

  3. Effects of quercetin, a natural phenolic compound, in the differentiation of human mesenchymal stem cells (MSC) into adipocytes and osteoblasts.

    Science.gov (United States)

    Casado-Díaz, Antonio; Anter, Jaouad; Dorado, Gabriel; Quesada-Gómez, José Manuel

    2016-06-01

    Natural phenols may have beneficial properties against oxidative stress, which is associated with aging and major chronic aging-related diseases, such as loss of bone mineral mass (osteoporosis) and diabetes. The main aim of this study was to analyze the effect of quercetin, a major nutraceutical compound present in the "Mediterranean diet", on mesenchymal stem-cell (MSC) differentiation. Such cells were induced to differentiate into osteoblasts or adipocytes in the presence of two quercetin concentrations (0.1 and 10μM). Several physiological parameters and the expression of osteoblastogenesis and adipogenesis marker genes were monitored. Quercetin (10μM) inhibited cell proliferation, alkaline phosphatase (ALPL) activity and mineralization, down-regulating the expression of ALPL, collagen type I alpha 1 (COL1A1) and osteocalcin [bone gamma-carboxyglutamate protein (BGLAP)] osteoblastogenesis-related genes in MSC differentiating into osteoblasts. Moreover, in these cultures, CCAAT/enhancer-binding protein alpha (CEBPA) and peroxisome proliferator-activated receptor gamma 2 (PPARG2) adipogenic genes were induced, and cells differentiated into adipocytes were observed. Quercetin did not affect proliferation, but increased adipogenesis, mainly at 10-μM concentration in MSC induced to differentiate to adipocytes. β- and γ-catenin (plakoglobin) nuclear levels were reduced and increased, respectively, in quercetin-treated cultures. This suggests that the effect of high concentration of quercetin on MSC osteoblastic and adipogenic differentiation is mediated via Wnt/β-catenin inhibition. In conclusion, quercetin supplementation inhibited osteoblastic differentiation and promoted adipogenesis at the highest tested concentration. Such possible adverse effects of high quercetin concentrations should be taken into account in nutraceutical or pharmaceutical strategies using such flavonol. PMID:27142748

  4. Ketangguhan Retak Dinamik Bahan Komposit GFRP Untuk Helmet Industri Disebabkan Beban Impak Menggunakan MSC/NASTRAN For Windows

    OpenAIRE

    Jusnita

    2010-01-01

    This research is to study computer simulation of glass fiber reinforced plastic (GFRP) plate specimen, by finite element method. Its goal is to know the stress intensity factor and compared them with the critical intensity factor (fracture toughanes/obtained using experimental method. The simulation utilized MSC MSN/NASTRAN for Window software and FEMAP sub program with impact load model. To simplify the simulation process, specimens were drawn in half because of its symmetric. Small meshes...

  5. Cell therapy medicinal product regulatory framework in Europe and its application for MSC-based therapy development.

    Science.gov (United States)

    Ancans, Janis

    2012-01-01

    Advanced therapy medicinal products (ATMPs), including cell therapy products, form a new class of medicines in the European Union. Since the ATMPs are at the forefront of scientific innovation in medicine, specific regulatory framework has been developed for these medicines and implemented from 2009. The Committee for Advanced Therapies (CAT) has been established at the European Medicines Agency (EMA) for centralized classification, certification and evaluation procedures, and other ATMP-related tasks. Guidance documents, initiatives, and interaction platforms are available to make the new framework more accessible for small- and medium-sized enterprises, academia, hospitals, and foundations. Good understanding of the centralized and national components of the regulatory system is required to plan product development. It is in the best interests of the cell therapy developers to utilize the resources provided starting with the pre-clinical stage. Whilst there have been no mesenchymal stem cell (MSC)-based medicine authorizations in the EU, three MSC products have received marketing approval in other regions since 2011. The information provided on the regulatory requirements, procedures, and initiatives is aimed at facilitating MSC-based medicinal product development and authorization in the EU.

  6. Treatment of radio-induced colorectal lesions with Mesenchymal Stromal Cells (MSC): part of the inflammatory process

    International Nuclear Information System (INIS)

    Throughout the last decades, radiotherapy established as a major tool in the treatment of abdomino-pelvic cancers. Despite great technological evolutions, radiotherapy remains associated with side effects that can sometimes be really harmful, this being mainly due to the toxicity of ionizing radiations for healthy tissues surrounding the tumor. As part of abdomino-pelvic radio-therapies, these side effects mainly affect the gastrointestinal tract, which is very sensitive to radiations. The development of curative treatments thus became a priority. Mesenchymal stem cells (MSC) showed their immunomodulatory ability as well as their ability to regenerate tissue in many models. During my thesis, we aimed at giving rise to the therapeutic advantage brought by MSC in the treatment of radioinduced damage as well as the underlying molecular mechanisms. Our results allowed us to demonstrate the efficiency of our treatment with an effect both on the colic epithelial structure and on its contractile functions. We demonstrated that this therapeutical efficiency depends on two processes. Stimulation of the epithelial proliferation through the Wnt pathway allows the epithelial regeneration process to be enhanced. The increased local corticosterone secretion allows the number and the activation state of T lymphocytes to diminish. Our results moreover suggest the existence of a link between the two observed phenomenons thus providing with a new proof of the combinatory effects of MSC therapy. (author)

  7. Towards an understanding of the structural and functional properties of MscL, a mechanosensitive channel in bacteria

    Science.gov (United States)

    Blount, P.; Sukharev, S. I.; Moe, P. C.; Nagle, S. K.; Kung, C.

    1996-01-01

    Whether it be to sense a touch, arterial pressure, or an osmotic gradient across a cell membrane, essentially all living organisms require the capability of detecting mechanical force. Electrophysiological evidence has suggested that mechanosensitive ion channels play a major role in many systems where mechanical force is detected. But, despite their biological importance, determination of the most basic structural and functional features of mechanosensitive channels has only recently become possible. A gene called mscL, which was isolated from Escherichia coli, was the first gene shown to encode a mechanosensitive channel activity. This channel directly responds to tension in the membrane; no other proteins are required. MscL appears to be a homohexamer of a 136 amino acid polypeptide that is highly alpha helical, contains two transmembrane domains, and has both the amino and carboxyl termini in the cytoplasm. The study of the MscL protein remains, to date, one of the most viable options for understanding the structural and functional characteristics of a mechanosensitive channel.

  8. The histopathology of a human mesenchymal stem cell experimental tumor model: support for an hMSC origin for Ewing's sarcoma?

    DEFF Research Database (Denmark)

    Burns, J S; Abdallah, B M; Schrøder, Henrik Daa;

    2008-01-01

    -forming potential of early passage hMSC-TERT20 cells, tumors derived from late passage cells expressed early biomarkers of osteogenesis. However, hMSC-TERT20 cells were heterogeneous for alpha smooth muscle actin (ASMA) expression and one out of six hMSC-TERT20 derived single cell clones was strongly ASMA positive....... Tumors from this ASMA+ clone had distinctive vascular qualities with hot spots of high CD34+ murine endothelial cell density, together with CD34- regions with a branching periodic acid Schiff reaction pattern. Such clone-specific differences in host vascular response provide novel models to explore...

  9. An MSC2 Promoter-lacZ Fusion Gene Reveals Zinc-Responsive Changes in Sites of Transcription Initiation That Occur across the Yeast Genome

    Science.gov (United States)

    Wu, Yi-Hsuan; Taggart, Janet; Song, Pamela Xiyao; MacDiarmid, Colin; Eide, David J.

    2016-01-01

    The Msc2 and Zrg17 proteins of Saccharomyces cerevisiae form a complex to transport zinc into the endoplasmic reticulum. ZRG17 is transcriptionally induced in zinc-limited cells by the Zap1 transcription factor. In this report, we show that MSC2 mRNA also increases (~1.5 fold) in zinc-limited cells. The MSC2 gene has two in-frame ATG codons at its 5’ end, ATG1 and ATG2; ATG2 is the predicted initiation codon. When the MSC2 promoter was fused at ATG2 to the lacZ gene, we found that unlike the chromosomal gene this reporter showed a 4-fold decrease in lacZ mRNA in zinc-limited cells. Surprisingly, β-galactosidase activity generated by this fusion gene increased ~7 fold during zinc deficiency suggesting the influence of post-transcriptional factors. Transcription of MSC2ATG2-lacZ was found to start upstream of ATG1 in zinc-replete cells. In zinc-limited cells, transcription initiation shifted to sites just upstream of ATG2. From the results of mutational and polysome profile analyses, we propose the following explanation for these effects. In zinc-replete cells, MSC2ATG2-lacZ mRNA with long 5’ UTRs fold into secondary structures that inhibit translation. In zinc-limited cells, transcripts with shorter unstructured 5’ UTRs are generated that are more efficiently translated. Surprisingly, chromosomal MSC2 did not show start site shifts in response to zinc status and only shorter 5’ UTRs were observed. However, the shifts that occur in the MSC2ATG2-lacZ construct led us to identify significant transcription start site changes affecting the expression of ~3% of all genes. Therefore, zinc status can profoundly alter transcription initiation across the yeast genome. PMID:27657924

  10. MSC Adams在双前桥转向机构设计中的应用%Application of MSC Adams in Design of Steering Mechanism of Double-Front Axle

    Institute of Scientific and Technical Information of China (English)

    吕召全; 华从波; 周福庚

    2006-01-01

    本论文对双前桥载货汽车的转向机构进行了全新的设计,在理论计算的基础上利用MSC Adams软件分别对转向梯形和转向摇臂机构进行了运动学仿真,并对其转向特性进行了优化. 随后的道路试验结果表明新设计的转向机构性能符合设计要求.

  11. Partial suppression of the respiratory defect of qrs1/her2 glutamyl-tRNA amidotransferase mutants by overexpression of the mitochondrial pentatricopeptide Msc6p.

    Science.gov (United States)

    Moda, Bruno S; Ferreira-Júnior, José Ribamar; Barros, Mario H

    2016-08-01

    Recently, a large body of evidences indicates the existence in the mitochondrial matrix of foci that contain different proteins involved in mitochondrial RNA metabolism. Some of these proteins have a pentatricopeptide repeat motif that constitutes their RNA-binding structures. Here we report that MSC6, a mitochondrial pentatricopeptide protein of unknown function, is a multi copy suppressor of mutations in QRS1/HER2 a component of the trimeric complex that catalyzes the transamidation of glutamyl-tRNAQ to glutaminyl-tRNAQ. This is an essential step in mitochondrial translation because of the lack of a specific mitochondrial aminoacyl glutaminyl-tRNA synthetase. MSC6 over-expression did not abolish translation of an aberrant variant form of Cox2p detected in QRS1/HER2 mutants, arguing against a suppression mechanism that bypasses Qrs1p function. A slight decrement of the mitochondrial translation capacity as well as diminished growth on respiratory carbon sources media for respiratory activity was observed in the msc6 null mutant. Additionally, the msc6 null mutant did not display any impairment in RNA transcription, processing or turnover. We concluded that Msc6p is a mitochondrial matrix protein and further studies are required to indicate the specific function of Msc6p in mitochondrial translation. PMID:26780366

  12. Partial suppression of the respiratory defect of qrs1/her2 glutamyl-tRNA amidotransferase mutants by overexpression of the mitochondrial pentatricopeptide Msc6p.

    Science.gov (United States)

    Moda, Bruno S; Ferreira-Júnior, José Ribamar; Barros, Mario H

    2016-08-01

    Recently, a large body of evidences indicates the existence in the mitochondrial matrix of foci that contain different proteins involved in mitochondrial RNA metabolism. Some of these proteins have a pentatricopeptide repeat motif that constitutes their RNA-binding structures. Here we report that MSC6, a mitochondrial pentatricopeptide protein of unknown function, is a multi copy suppressor of mutations in QRS1/HER2 a component of the trimeric complex that catalyzes the transamidation of glutamyl-tRNAQ to glutaminyl-tRNAQ. This is an essential step in mitochondrial translation because of the lack of a specific mitochondrial aminoacyl glutaminyl-tRNA synthetase. MSC6 over-expression did not abolish translation of an aberrant variant form of Cox2p detected in QRS1/HER2 mutants, arguing against a suppression mechanism that bypasses Qrs1p function. A slight decrement of the mitochondrial translation capacity as well as diminished growth on respiratory carbon sources media for respiratory activity was observed in the msc6 null mutant. Additionally, the msc6 null mutant did not display any impairment in RNA transcription, processing or turnover. We concluded that Msc6p is a mitochondrial matrix protein and further studies are required to indicate the specific function of Msc6p in mitochondrial translation.

  13. Software Realization on the MSC nanoRISC Hardware Platform, for Communication according to the IEC61850 Standard

    Directory of Open Access Journals (Sweden)

    A. V. Kabović

    2015-06-01

    Full Text Available This paper describes software realization and its implementation for the communication, according to the IEC61850 standard, between the module for monitoring teleprotection devices and the control/monitoring server in a power substation. Teleprotection devices have an important role in the transmission of messages for power line section tripping. The software is implemented on the “MSC nanoRISC-S3C2416 MB2” hardware platform type, which belongs to the COM (computer on module systems.

  14. Effect of mesenchymal stromal cells (MSC) on chronic visceral hypersensitivity in a radio-induced colonic ulceration model in the rat

    International Nuclear Information System (INIS)

    Patients who undergo pelvic radiotherapy may develop significant incidence of undesirable chronic gastrointestinal complications resulting from radiation-induced damages around the tumour. Chronic visceral pain is one of the radiation-induced side effects that greatly affects the quality of life of 'cancer survivors'. The lack of effective analgesic treatment highlights the importance of novel and effective therapeutic strategies. In our laboratory, mesenchymal stromal cell (MSC) based approach showed beneficial immunomodulatory and regenerative effects in a rat model of irreversible radiationinduced colonic ulcers. The goal of my work was to assess the relevance of this model to study radiation-induced visceral persistent hypersensitivity and its modulation by MSC treatment. We first demonstrated that this model is associated with long-lasting visceral hypersensitivity and central neuronal sensitization. In this context we showed then that mast cells (MC) are involved in the mechanism of peripheral sensitization. Moreover, we suggested the implication of the neuro-mediator NO. in the pathophysiology of persistent radiation-induced visceral hypersensitivity. We also suggested that MSC treatment reversed radiation-induced hypersensitivity by a mechanism that in part may involve the modulation of MC activation and/or the decrease in the number of MC and nerve fiber interactions. In addition, MSC treatment reduced the percentage of nitrinergic neurons, increased after irradiation, and restored colonic muscular contractibility. Such processes may promote the therapeutic benefit of MSC observed in our study. In conclusion, this work provided new insights on the therapeutic benefit of MSC in our study model and a new argument in favour of their use in a future clinical trial to cure abdomino-pelvic radiotherapy side effects. (author)

  15. Therapeutic potential of mesenchymal stromal cells and MSC conditioned medium in Amyotrophic Lateral Sclerosis (ALS--in vitro evidence from primary motor neuron cultures, NSC-34 cells, astrocytes and microglia.

    Directory of Open Access Journals (Sweden)

    Hui Sun

    Full Text Available Administration of mesenchymal stromal cells (MSC improves functional outcome in the SOD1G93A mouse model of the degenerative motor neuron disorder amyotrophic lateral sclerosis (ALS as well as in models of other neurological disorders. We have now investigated the effect of the interaction between MSC and motor neurons (derived from both non-transgenic and mutant SOD1G93A transgenic mice, NSC-34 cells and glial cells (astrocytes, microglia (derived again from both non-transgenic and mutant SOD1G93A ALS transgenic mice in vitro. In primary motor neurons, NSC-34 cells and astrocytes, MSC conditioned medium (MSC CM attenuated staurosporine (STS - induced apoptosis in a concentration-dependent manner. Studying MSC CM-induced expression of neurotrophic factors in astrocytes and NSC-34 cells, we found that glial cell line-derived neurotrophic factor (GDNF and ciliary neurotrophic factor (CNTF gene expression in astrocytes were significantly enhanced by MSC CM, with differential responses of non-transgenic and mutant astrocytes. Expression of Vascular Endothelial Growth Factor (VEGF in NSC-34 cells was significantly upregulated upon MSC CM-treatment. MSC CM significantly reduced the expression of the cytokines TNFα and IL-6 and iNOS both in transgenic and non-transgenic astrocytes. Gene expression of the neuroprotective chemokine Fractalkine (CX3CL1 was also upregulated in mutant SOD1G93A transgenic astrocytes by MSC CM treatment. Correspondingly, MSC CM increased the respective receptor, CX3CR1, in mutant SOD1G93A transgenic microglia. Our data demonstrate that MSC modulate motor neuronal and glial response to apoptosis and inflammation. MSC therefore represent an interesting candidate for further preclinical and clinical evaluation in ALS.

  16. Comparison of composite rotor blade models: A coupled-beam analysis and an MSC/NASTRAN finite-element model

    Science.gov (United States)

    Hodges, Robert V.; Nixon, Mark W.; Rehfield, Lawrence W.

    1987-01-01

    A methodology was developed for the structural analysis of composite rotor blades. This coupled-beam analysis is relatively simple to use compared with alternative analysis techniques. The beam analysis was developed for thin-wall single-cell rotor structures and includes the effects of elastic coupling. This paper demonstrates the effectiveness of the new composite-beam analysis method through comparison of its results with those of an established baseline analysis technique. The baseline analysis is an MSC/NASTRAN finite-element model built up from anisotropic shell elements. Deformations are compared for three linear static load cases of centrifugal force at design rotor speed, applied torque, and lift for an ideal rotor in hover. A D-spar designed to twist under axial loading is the subject of the analysis. Results indicate the coupled-beam analysis is well within engineering accuracy.

  17. Input Files and Procedures for Analysis of SMA Hybrid Composite Beams in MSC.Nastran and ABAQUS

    Science.gov (United States)

    Turner, Travis L.; Patel, Hemant D.

    2005-01-01

    A thermoelastic constitutive model for shape memory alloys (SMAs) and SMA hybrid composites (SMAHCs) was recently implemented in the commercial codes MSC.Nastran and ABAQUS. The model is implemented and supported within the core of the commercial codes, so no user subroutines or external calculations are necessary. The model and resulting structural analysis has been previously demonstrated and experimentally verified for thermoelastic, vibration and acoustic, and structural shape control applications. The commercial implementations are described in related documents cited in the references, where various results are also shown that validate the commercial implementations relative to a research code. This paper is a companion to those documents in that it provides additional detail on the actual input files and solution procedures and serves as a repository for ASCII text versions of the input files necessary for duplication of the available results.

  18. Heparin-induced conformational changes of fibronectin within the extracellular matrix promote hMSC osteogenic differentiation.

    Science.gov (United States)

    Li, Bojun; Lin, Zhe; Mitsi, Maria; Zhang, Yang; Vogel, Viola

    2015-01-01

    An increasing body of evidence suggests important roles of extracellular matrix (ECM) in regulating stem cell fate. This knowledge can be exploited in tissue engineering applications for the design of ECM scaffolds appropriate to direct stem cell differentiation. By probing the conformation of fibronectin (Fn) using fluorescence resonance energy transfer (FRET), we show here that heparin treatment of the fibroblast-derived ECM scaffolds resulted in more extended conformations of fibrillar Fn in ECM. Since heparin is a highly negatively charged molecule while fibronectin contains segments of positively charged modules, including FnIII13, electrostatic interactions between Fn and heparin might interfere with residual quaternary structure in relaxed fibronectin fibers thereby opening up buried sites. The conformation of modules FnIII12-14 in particular, which contain one of the heparin binding sites as well as binding sites for many growth factors, may be activated by heparin, resulting in alterations in growth factor binding to Fn. Indeed, upregulated osteogenic differentiation was observed when hMSCs were seeded on ECM scaffolds that had been treated with heparin and were subsequently chemically fixed. In contrast, either rigidifying relaxed fibers by fixation alone, or heparin treatment without fixation had no effect. We hypothesize that fibronectin's conformations within the ECM are activated by heparin such as to coordinate with other factors to upregulate hMSC osteogenic differentiation. Thus, the conformational changes of fibronectin within the ECM could serve as a 'converter' to tune hMSC differentiation in extracellular matrices. This knowledge could also be exploited to promote osteogenic stem cell differentiation on biomedical surfaces. PMID:26214191

  19. Spot14/Spot14R expression may be involved in MSC adipogenic differentiation in patients with adolescent idiopathic scoliosis

    Science.gov (United States)

    WANG, QIFEI; YANG, JUNLIN; LIN, XIANG; HUANG, ZIFANG; XIE, CHAOFAN; FAN, HENGWEI

    2016-01-01

    The aim of the present study was to evaluate the different expression levels of thyroid hormone responsive (THRSP; Spot14)/S14 related, Mig12 (S14R) during bone marrow mesenchymal stem cell (BM-MSC) adipogenesis in adolescent idiopathic scoliosis (AIS) patients. MSCs were retrospectively isolated from AIS patients and controls, and adipogenic differentiation was induced. Total RNA was extracted for Affymetrix 3′-IVT expression profiling microarrays and compared with the results from healthy controls. The results were confirmed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) validation and the protein expression levels of Spot14 and its paralogous gene S14R by western blotting and immunohistochemistry. A total of 300 significantly altered mRNAs were detected (111 upregulated and 189 downregulated) and confirmed by RT-qPCR. The mRNA expression levels of seven genes, including Spot14, were altered by >2-fold in AIS patients. Spot14/S14R was selected for further investigation. The results of the western blotting demonstrated that mRNA and protein expression levels of Spot14/S14R were significantly higher in AIS patients than the controls (P<0.05). Immunohistochemistry demonstrated Spot14 was expressed in 85% (17/20 cases) in adipose tissue samples from AIS patients and 23.1% (3/13 cases) of adipose tissue samples from controls. The positive ratio of Spot14 in adipose tissue samples from AIS was significantly higher than the controls (P<0.001). The results of the present study indicated that Spot14/S14R were differently expressed in MSC adipogenesis in AIS patients, and they may be important in the abnormal adipogenic differentiation in AIS. PMID:27082501

  20. Cytoplasmic Domain of MscS Interacts with Cell Division Protein FtsZ: A Possible Non-Channel Function of the Mechanosensitive Channel in Escherichia Coli.

    Directory of Open Access Journals (Sweden)

    Piotr Koprowski

    Full Text Available Bacterial mechano-sensitive (MS channels reside in the inner membrane and are considered to act as emergency valves whose role is to lower cell turgor when bacteria enter hypo-osmotic environments. However, there is emerging evidence that members of the Mechano-sensitive channel Small (MscS family play additional roles in bacterial and plant cell physiology. MscS has a large cytoplasmic C-terminal region that changes its shape upon activation and inactivation of the channel. Our pull-down and co-sedimentation assays show that this domain interacts with FtsZ, a bacterial tubulin-like protein. We identify point mutations in the MscS C-terminal domain that reduce binding to FtsZ and show that bacteria expressing these mutants are compromised in growth on sublethal concentrations of β-lactam antibiotics. Our results suggest that interaction between MscS and FtsZ could occur upon inactivation and/or opening of the channel and could be important for the bacterial cell response against sustained stress upon stationary phase and in the presence of β-lactam antibiotics.

  1. One-step derivation of mesenchymal stem cell (MSC-like cells from human pluripotent stem cells on a fibrillar collagen coating.

    Directory of Open Access Journals (Sweden)

    Yongxing Liu

    Full Text Available Controlled differentiation of human embryonic stem cells (hESCs and induced pluripotent stem cells (iPSCs into cells that resemble adult mesenchymal stem cells (MSCs is an attractive approach to obtain a readily available source of progenitor cells for tissue engineering. The present study reports a new method to rapidly derive MSC-like cells from hESCs and hiPSCs, in one step, based on culturing the cells on thin, fibrillar, type I collagen coatings that mimic the structure of physiological collagen. Human H9 ESCs and HDFa-YK26 iPSCs were singly dissociated in the presence of ROCK inhibitor Y-27632, plated onto fibrillar collagen coated plates and cultured in alpha minimum essential medium (alpha-MEM supplemented with 10% fetal bovine serum, 50 uM magnesium L-ascorbic acid phosphate and 100 nM dexamethasone. While fewer cells attached on the collagen surface initially than standard tissue culture plastic, after culturing for 10 days, resilient colonies of homogenous spindle-shaped cells were obtained. Flow cytometric analysis showed that a high percentage of the derived cells expressed typical MSC surface markers including CD73, CD90, CD105, CD146 and CD166 and were negative as expected for hematopoietic markers CD34 and CD45. The MSC-like cells derived from pluripotent cells were successfully differentiated in vitro into three different lineages: osteogenic, chondrogenic, and adipogenic. Both H9 hES and YK26 iPS cells displayed similar morphological changes during the derivation process and yielded MSC-like cells with similar properties. In conclusion, this study demonstrates that bioimimetic, fibrillar, type I collagen coatings applied to cell culture plates can be used to guide a rapid, efficient derivation of MSC-like cells from both human ES and iPS cells.

  2. Medialogy (English Information Brochure on the education M.Sc.,Medialogy, 3rd to 10th semester (B.Sc. & M.Sc) - For graduates from higher study programmes such as Multimedia Design and IT Graduates)

    DEFF Research Database (Denmark)

    Nordahl, Rolf

    2005-01-01

    An information brochure, 24 pages, in English which describes the education M.Sc., Medialogy, offered by Aalborg University in Copenhagen and Esbjerg. The brochure describes the 3rd to 10th semester (B.Sc. and M.Sc. degree) of the education. The information is primarily directed toward potential...

  3. Inter-professional work based learning within an MSc in Advanced Practice: lessons from one UK higher education programme.

    Science.gov (United States)

    Gaskell, Lynne; Beaton, Susan

    2010-09-01

    This paper will describe the implementation of inter-professional work based education (IPE) in one postgraduate Advanced Practitioner programme in the UK. The concept of Advanced Practice has developed as a response of a number of drivers including change in junior doctor training; government policy and increasing demands on the central government funded UK health service (the NHS). The programme was commissioned by the then greater Manchester Strategic Health Authority (now NHS North West) to meet service needs. The educational philosophy underpinning the MSc Advanced Practice (health and social care) provided by the University of Salford is IPE linked to work based learning. The process of work based learning (WBL) and inter-professional learning underpinning the programme will be discussed in relation to feedback from university staff, Advanced Practitioner (AP) students and employer feedback taken from programme and module evaluations. We argue that IPE at this level facilitates a greater understanding of the connectivity between professionals working in the health care system in the UK; a better understanding of the skills and knowledge base of colleagues; more inter-professional working and appropriate referrals in the work place. This has raised the profile of Advanced Practice (AP) in the region and ultimately resulted in better patient care with more effective and efficient use of resources (Acton Shapiro, 2006, 2008).

  4. Chimeras Reveal a Single Lipid-Interface Residue that Controls MscL Channel Kinetics as well as Mechanosensitivity

    Directory of Open Access Journals (Sweden)

    Li-Min Yang

    2013-02-01

    Full Text Available MscL, the highly conserved bacterial mechanosensitive channel of large conductance, serves as an osmotic “emergency release valve,” is among the best-studied mechanosensors, and is a paradigm of how a channel senses and responds to membrane tension. Although all homologs tested thus far encode channel activity, many show functional differences. We tested Escherichia coli and Staphylococcus aureus chimeras and found that the periplasmic region of the protein, particularly E. coli I49 and the equivalent S. aureus F47 at the periplasmic lipid-aqueous interface of the first transmembrane domain, drastically influences both the open dwell time and the threshold of channel opening. One mutant shows a severe hysteresis, confirming the importance of this residue in determining the energy barriers for channel gating. We propose that this site acts similarly to a spring for a clasp knife, adjusting the resistance for obtaining and stabilizing an open or closed channel structure.

  5. 2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS.

    Science.gov (United States)

    Akitake, Bradley; Spelbrink, Robin E J; Anishkin, Andriy; Killian, J Antoinette; de Kruijff, Ben; Sukharev, Sergei

    2007-04-15

    2,2,2-Trifluoroethanol (TFE), a low-dielectric solvent, has recently been used as a promising tool to probe the strength of intersubunit interactions in membrane proteins. An analysis of inner membrane proteins of Escherichia coli has identified several SDS-resistant protein complexes that separate into subunits upon exposure to TFE. One of these was the homo-heptameric stretch-activated mechanosensitive channel of small conductance (MscS), a ubiquitous component of the bacterial turgor-regulation system. Here we show that a substantial fraction of MscS retains its oligomeric state in cold lithium-dodecyl-sulfate gel electrophoresis. Exposure of MscS complexes to 10-15 vol % TFE in native membranes or nonionic detergent micelles before lithium-dodecyl-sulfate electrophoresis results in a complete dissociation into monomers, suggesting that at these concentrations TFE by itself disrupts or critically compromises intersubunit interactions. Patch-clamp analysis of giant E. coli spheroplasts expressing MscS shows that exposure to TFE in lower concentrations (0.5-5.0 vol %) causes leftward shifts of the dose-response curves when applied extracellularly, and rightward shifts when added from the cytoplasmic side. In the latter case, TFE increases the rate of tension-dependent inactivation and lengthens the process of recovery to the resting state. MscS responses to pressure ramps of different speeds indicate that in the presence of TFE most channels reside in the resting state and only at tensions near the activation threshold does TFE dramatically speed up inactivation. The effect of TFE is reversible as normal channel activity returns 15-30 min after a TFE washout. We interpret the observed midpoint shifts in terms of asymmetric partitioning of TFE into the membrane and distortion of the bilayer lateral pressure profile. We also relate the increased rate of inactivation and subunit separation with the capacity of TFE to perturb buried interhelical contacts in proteins

  6. Phenanthroline treated MSC

    NARCIS (Netherlands)

    Fernandes, H.A.M.; Boer, de J.; Quax, P.H.A.; Doorn, J.

    2013-01-01

    The invention relates to the field of medicine and in particular to the treatment of ischemia and to angiogenesis. The invention further relates to novel uses of phenanthroline in replacing a missing biological structure, supporting a damaged biological structure and/or enhancing an existing biologi

  7. Half-metallic ferromagnetism in chalcopyrite type compounds ZnMX{sub 2} (M=Sc, V, Mn, Fe; X = P, As)

    Energy Technology Data Exchange (ETDEWEB)

    Vijayalakshmi, D.; Kalpana, G., E-mail: g-kalpa@yahoo.com, E-mail: g-kalpa@annauniv.edu [Department of Physics, Anna University, Chennai – 600025 (India)

    2015-06-24

    Electronic structure and magnetic properties of ZnMX{sub 2} (M=Sc, V, Mn and Fe; X= As and P) compounds in body centred tetragonal chalcopyrite structure have been investigated using first-principles calculations based on density functional theory (DFT) within the local spin density approximation (LSDA). The spin-polarized electronic band structure and density of states of all these compounds show that the spin-up electrons have metallic and the spin-down electrons have a semiconducting gap and the magnetic moment mainly originates from the strong spin polarization of 3d states of transition metal (M=Sc, V, Mn and Fe) atoms and p-like states of anion X (P and As) atoms.

  8. Intranasal administration of human MSC for ischemic brain injury in the mouse: in vitro and in vivo neuroregenerative functions.

    Directory of Open Access Journals (Sweden)

    Vanessa Donega

    Full Text Available Intranasal treatment with C57BL/6 MSCs reduces lesion volume and improves motor and cognitive behavior in the neonatal hypoxic-ischemic (HI mouse model. In this study, we investigated the potential of human MSCs (hMSCs to treat HI brain injury in the neonatal mouse. Assessing the regenerative capacity of hMSCs is crucial for translation of our knowledge to the clinic. We determined the neuroregenerative potential of hMSCs in vitro and in vivo by intranasal administration 10 d post-HI in neonatal mice. HI was induced in P9 mouse pups. 1×10(6 or 2×10(6 hMSCs were administered intranasally 10 d post-HI. Motor behavior and lesion volume were measured 28 d post-HI. The in vitro capacity of hMSCs to induce differentiation of mouse neural stem cell (mNSC was determined using a transwell co-culture differentiation assay. To determine which chemotactic factors may play a role in mediating migration of MSCs to the lesion, we performed a PCR array on 84 chemotactic factors 10 days following sham-operation, and at 10 and 17 days post-HI. Our results show that 2×10(6 hMSCs decrease lesion volume, improve motor behavior, and reduce scar formation and microglia activity. Moreover, we demonstrate that the differentiation assay reflects the neuroregenerative potential of hMSCs in vivo, as hMSCs induce mNSCs to differentiate into neurons in vitro. We also provide evidence that the chemotactic factor CXCL10 may play an important role in hMSC migration to the lesion site. This is suggested by our finding that CXCL10 is significantly upregulated at 10 days following HI, but not at 17 days after HI, a time when MSCs no longer reach the lesion when given intranasally. The results described in this work also tempt us to contemplate hMSCs not only as a potential treatment option for neonatal encephalopathy, but also for a plethora of degenerative and traumatic injuries of the nervous system.

  9. 基于MSC-51单片机的智能压力变送器%Design of the intelligent pressure transducer based on 51 SOC

    Institute of Scientific and Technical Information of China (English)

    张秀国

    2013-01-01

    Along with the requirements of industrial application of signal detection and transmission continues to improve,new intelligent instrument will play more and more important role in the market.Based on the analysis of the basic principle of pressure transducer on the work under the new situation,according to the production requirements,design a data acquisition circuit,the intelligent pressure transmitter MSC-51 microcontroller watchdog circuit and interface circuit based on.And design the corresponding algorithm of data collection,communication protocol and software function.%随着工业应用对信号检测与传输的要求不断提高,新型智能仪表将在市场中占有越来越重要的地位.本文在分析压力变送器基本工作原理的基础上,针对新形势下的生产要求,设计了基于MSC-51单片机的智能压力变送器的数据采集电路、看门狗电路以及接口电路.并设计了相应的数据采集算法、通信协议以及其他软件功能.

  10. Changes in SeMSC, Glucosinolates and Sulforaphane Levels, and in Proteome Profile in Broccoli (Brassica oleracea var. Italica Fertilized with Sodium Selenate

    Directory of Open Access Journals (Sweden)

    Alejandra Moenne

    2013-05-01

    Full Text Available The aim of this work was to analyze the effect of sodium selenate fortification on the content of selenomethyl selenocysteine (SeMSC, total glucosinolates and sulforaphane, as well as the changes in protein profile of the inflorescences of broccoli (Brassica oleracea var. Italica. Two experimental groups were considered: plants treated with 100 mmol/L sodium selenate (final concentration in the pot and control plants treated with water. Fortification began 2 weeks after transplantation and was repeated once a week during 10 weeks. Broccoli florets were harvested when they reached appropriate size. SeMSC content in broccoli florets increased significantly with sodium selenate fortification; but total glucosinolates and sulforaphane content as well as myrosinase activity were not affected. The protein profile of broccoli florets changed due to fortification with sodium selenate. Some proteins involved in general stress-responses were up-regulated, whereas down-regulated proteins were identified as proteins involved in protection against pathogens. This is the first attempt to evaluate the physiological effect of fortification with sodium selenate on broccoli at protein level. The results of this work will contribute to better understanding the metabolic processes related with selenium uptake and accumulation in broccoli.

  11. Strength Analysis of Sandwitch Panels with CFRP Quasi Isotropic Layup or Isotropic Material Facesheets in Dynamic Environment Using MSC Nastran and SineMOS

    Science.gov (United States)

    Di Carlo, A.; Carbonell Garcia, A.

    2012-07-01

    The frequency response solution (SOL 111) of MSC Nastran versions prior to 2012 only allows the output of element stress components and element forces and does not allow the calculation of composite failure indices or Von-Mises stress for metallic parts. The analysis of a sandwich panel comprises several strength verifications, such as the check of facesheet and core failure as well as the check of facesheet and core local stability (shear crimping, wrinkling). In static analysis (SOL 101), MSC Nastran provides failure index output which can be used to generate fringe plots of Margins of Safety (MoS) in any post- processing tool. The other verifications (core strength and local stability) must be performed using different tools. For the dynamic analysis of sandwich panels, an analysis technique based on element forces and on failure envelope at laminate level has been developed and implemented in a Fortran program (SineMOS) which allows evaluating facesheet and core failure as well as local stability, taking into account modulus and phase information of the element forces. SineMOS is able to produce files containing information used to generate plots of minimum Margin of Safety in Patran for each failure mode. This paper shows the various steps of the analysis process, starting from the building of the failure envelope for the CFRP facesheet laminate. Finally some validation example is shown, comparing SineMOS results with results based on the application of static displacements to the nodes of the model.

  12. A critical role of IFNγ in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Bone-marrow-derived mesenchymal stem cells (MSCs) have been shown to possess immunosuppressive properties, e.g., by inhibiting T cell proliferation. Activated T cells can also enhance the immunosuppression ability of MSCs. The precise mechanisms underlying MSC-mediated immunosuppression remain largely undefined, although both cell-cell contact and soluble factors have been implicated; nor is it clear how the immunosuppressive property of MSCs is modulated by T cells. Using MSCs isolated from mouse bone marrow, we show here that interferon gamma (IFNγ), a well-known proinflammatory cytokine produced by activated T cells, plays an important role in priming the immunosuppressive property of MSCs. Mechanistically, IFNγ acts directly on MSCs and leads to up-regulation of B7-H1, an inhibitory surface molecule in these stem cells. MSCs primed by activated T cells derived from IFNγ-/- mouse exhibited dramatically reduced ability to suppress T cell proliferation, a defect that can be rescued by supplying exogenous IFNy. Moreover, siRNA-mediated knockdown of B7-H1 in MSCs abolished immunosuppression by these cells. Taken together, our results suggest that IFNy plays a critical role in triggering the immunosuppresion by MSCs through upregulating B7-H1 in these cells, and provide evidence supporting the cell-cell contact mechanism in MSC-mediated immunosuppression.

  13. Past changes of the North African monsoon intensity between 5 and 6.2 My, impact of the Messinian Salinity Crisis (MSC)

    Science.gov (United States)

    Ségueni, F.; Colin, C.; Siani, G.; Frank, N.; Blamart, D.; Kissel, C.; Liu, Z.; Richter, T.; Suc, J.

    2006-12-01

    A high resolution multiproxy study by oxygen isotope record (δ18O) on benthic foraminifera (Cibicides wuellerstorfii), magnetic susceptibility, clay mineralogy (DRX), major - trace elements (XRF core scanner and ICPMS) and Rb/Sr - Nd isotopes was carried out from site ODP 659 along the Cape Verde off Africa. The aim was to reconstruct variations of African Monsoon during the Mio-Pliocene in the time interval from 5 My to 6,2 My. Chronology was established by linear interpolation between 3 bio-events based on calcareous nannoplancton zones, 2 glacial stages TG12 and TG22 identified on δ18O records and by tuning the δ18O and magnetic susceptibility records to the orbital parameter of obliquity and precession. Results indicate that between 5 to 6.2 My variability in the eolian input from Sahara and the coastal upwelling intensity are anti-correlated and make it possible to retrace the evolution of northern African Monsoon. The latter co- varies mainly with the insolation received by the earth at low latitude during the summer. Maximal insolation enhance summer monsoonal effects by increasing wetter conditions on Sahel and NE dominance wind system cause a reduced eolian input and an increased biogenic sea surface productivity by coastal upwelling. On the other hand, minimal insolation reinforce winter monsoon that create a more arid climate on Sahel and stronger westward winds that increase eolian flux on Cap Verde with a reduced upwelling effect on sea surface productivity. At a longer time scale, the end of the MSC is correlated with a major change of the African Monsoon intensity. Finally, the δ18O record on C.wuellerstorfii suggests that global eustatic processes didn't play a key role in the MSC history. Nevertheless, transition between glacial stage TG12 and the interglacial TG11 seems to correspond to a major event within the MSC, and associated to the beginning of the upper evaporite deposits. Thus, the facies of the Lago Mare of the upper evaporites would

  14. Preliminary Analysis of the Social and Scientific Impact of the UAEM-ININ M.Sc. and D.Sc. Graduate Programme in Medical Physics

    Science.gov (United States)

    Mitsoura, Eleni; Isaac-Olive, Keila; Torres-Garcia, Eugenio; Camacho-Lopez, Miguel Angel; Hardy-Perez, Alberto

    2010-12-01

    Sponsored by the International Atomic Energy Agency (IAEA) in 1994, the Instituto Nacional de Investigaciones Nucleares (ININ) started in Mexico a teaching and training programme (Diplomado) in Radiotherapy Medical Physics. Based on this experience, the Universidad Autónoma del Estado de México (UAEM) and the Instituto Nacional de Investigaciones Nucleares (ININ) launched two years later, the first Graduate Programme in Science (M.Sc. and D.Sc.), specialised in Medical Physics in Mexico. A preliminary analysis of the social and scientific impact of the UAEM-ININ Programme is presented in this work based on the achievements attained, regarding the number of graduated Medical Physicists, their geographic and academic origin, their current professional activities and the number of scientific publications produced as a result of the thesis, as well as their citations.

  15. Application Progress of MSC-stents Complex in Bone Repair%间充质干细胞-支架复合体运用于骨修复的研究进展

    Institute of Scientific and Technical Information of China (English)

    刘印

    2013-01-01

    Bone defect is a common complication after fracture, and how to effectively repair it is the hot topic in the current medical study. The clinical application of bone tissue engineering technology is gradually rising,and MSC-stents complex can effectively combine their advantages,which is beneficial to the treatment of bone defect. Here is to summarize the MSC-stents requirements, such as cell number, microenvironment simulation and related growth factor function etc. ,to provide a reference basis for further studies.%骨缺损是骨折后的常见并发症,如何有效地进行骨修复是当前医学研究的热点.骨组织工程技术在临床上应用逐渐兴起,间充质干细胞(MSC)-支架复合体可有效结合干细胞与支架的骨修复优势,利于骨缺损的治疗.该文总结分析有关MSC-支架复合体条件需求,如细胞数量,微环境模拟,相关生长因子作用等,为今后的深入研究提供参考.

  16. Therapeutic use of human mesenchymal stem cells (MSC) for the treatment of radio-induced diseases; Utilisation des Cellules Souches Mesenchymateuses humaines dans le traitement des atteintes tissulaires radio-induites

    Energy Technology Data Exchange (ETDEWEB)

    Mouiseddine, Moubarak

    2008-05-15

    Ionising radiation can induce toxic effects on body. They provoke physiological modifications of tissues and organs which can be lethal. Total body irradiation or local abdominal irradiation can induce serious complications. Intestine is the first tissue concerned by these side effects. Radiation induces malabsorption of the intestine and lost of it integrity. Radio-induced physiopathological effects on intestine could lead to distant effects on other tissues and organs such as liver. The actual treatments have a limited efficiency or are not adapted to gastrointestinal damages. Indeed, in this type of lesions, the heterogeneous systems which are concerned and the gravity of lesions complicates the medical care. Our purpose is to show that cell therapy using human mesenchymal stem cells (MSC) constitutes resolution in this type of illness. The works which are presented in this thesis show that MSC are multi-potent and have heterogeneous expression of molecules. These cells are able to establish themselves in many organs and tissues after injection into irradiated body. Thus we have shown that MSC can prevent the small intestine from radio-induced damages. Indeed we demonstrate that through their actions on gut, MSC can indirectly restore hepatic integrity. (author)

  17. Crystal structures and magnetic properties of lanthanide containing borates LnM(BO{sub 3}){sub 2} (Ln=Y, Ho–Lu; M=Sc, Cr)

    Energy Technology Data Exchange (ETDEWEB)

    Doi, Yoshihiro, E-mail: doi@sci.hokudai.ac.jp; Satou, Tatsuya; Hinatsu, Yukio

    2013-10-15

    The synthesis, crystal structures and magnetic properties of LnM(BO{sub 3}){sub 2} (Ln=Y, Ho–Lu; M=Sc, Cr) were investigated. The LnCr(BO{sub 3}){sub 2} compounds crystallize in the dolomite-type structure with space group R3{sup ¯}, in which the Ln and Cr ions occupy two octahedral sites. From the result of structural analysis, it was found that there is an anti-site disorder between these two sites and its chemical formula is more exactly Ln{sub 1−r}Cr{sub r}[Cr{sub 1−r}Ln{sub r}](BO{sub 3}){sub 2}. On the other hand, the LnSc(BO{sub 3}){sub 2} adopt the calcite-type structure with space group R3{sup ¯}c. The Ln and Sc ions randomly occupy an octahedral site and the chemical formula is represented as (Ln{sub 0.5}Sc{sub 0.5})BO{sub 3}. From the magnetic susceptibility and specific heat measurements, we found that all the LnCr(BO{sub 3}){sub 2} show an antiferromagnetic transition at 6.1–8.1 K. This transition is mainly due to the ordering of Cr{sup 3+} magnetic moments. Among the compounds with magnetic Ln{sup 3+} ions, only YbCr(BO{sub 3}){sub 2} shows an antiferromagnetic ordering of Ln{sup 3+} ion at 2.1 K. - Graphical abstract: The lanthanide containing borates LnM(BO{sub 3}){sub 2} (Ln=Y, Ho–Lu; M=Sc, Cr) have the dolomite-type (Ln=Cr) and calcite-type (Ln=Sc) structures. Both structures are similar to each other except for the difference in the partially or fully disordered arrangements of octahedral sites. At low temperatures, the LnCr(BO{sub 3}){sub 2} compounds show an antiferromagnetic transition due to a long-range ordering of Cr{sup 3+} moments. Among them only YbCr(BO{sub 3}){sub 2} shows an antiferromagnetic ordering of Ln{sup 3+} ion at 2.1 K. Display Omitted - Highlights: • Lanthanide containing borates LnM(BO{sub 3}){sub 2} (Ln=Y, Ho–Lu; M=Sc, Cr) have been synthesized. • LnCr(BO{sub 3}){sub 2} has the dolomite-type structure with an anti-site disorder between Ln and Cr sites. • LnSc(BO{sub 3}){sub 2} has the calcite

  18. MSC Rescues Under-Fives.

    Science.gov (United States)

    Webberley, Jill

    1981-01-01

    This article describes the Milton Keynes Home-School Link project, which provides home instruction for parents with preschoolers. Funded by the Manpower Services Commission, the project provides short-term employment for out-of-work teachers, who serve as home visitors, and unemployed young people, who act as aides. (SJL)

  19. Influence of electrospun fiber mesh size on hMSC oxygen metabolism in 3D collagen matrices: experimental and theoretical evidences.

    Science.gov (United States)

    Guaccio, Angela; Guarino, Vincenzo; Perez, Marco A Alvarez-; Cirillo, Valentina; Netti, Paolo A; Ambrosio, Luigi

    2011-08-01

    The traditional paradigm of tissue engineering of regenerating in vitro tissue or organs, through the combination of an artificial matrix and a cellular population has progressively changed direction. The most recent concept is the realization of a fully functional biohybrid, where both, the artificial and the biotic phase, concur in the formation of the novel organic matter. In this direction, interest is growing in approaches taking advantage of the control at micro- and nano-scale of cell material interaction based on the realization of elementary tassels of cells and materials which constitute the beginning point for the expansion of 3D more complex structures. Since a spontaneous assembly of all these components is expected, however, it becomes more fundamental than ever to define the features influencing cellular behavior, either they were material functional properties, or material architecture. In this work, it has been investigated the direct effect of electrospun fiber sizes on oxygen metabolism of h-MSC cells, when any other culture parameter was kept constant. To this aim, thin PCL electrospun membranes, with micro- and nano-scale texturing, were layered between two collagen slices up to create a sandwich structure (µC-PCL-C and nC-PCL-C). Cells were seeded on membranes, and the oxygen consumption was determined by a phosphorescence quenching technique. Results indicate a strong effect of the architecture of scaffolds on cell metabolism, also revealed by the increasing of HIF1-α gene expression in nC-PCL-C. These findings offer new insights into the role of materials in specific cell activities, also implying the existence of very interesting criteria for the control of tissue growth through the tuning of scaffold architecture.

  20. Degenerate wave and capacitive coupling increase human MSC invasion and proliferation while reducing cytotoxicity in an in vitro wound healing model.

    Directory of Open Access Journals (Sweden)

    Michelle Griffin

    Full Text Available Non-unions pose complications in fracture management that can be treated using electrical stimulation (ES. Bone marrow mesenchymal stem cells (BMMSCs are essential in fracture healing; however, the effect of different clinical ES waveforms on BMMSCs cellular activities remains unknown. We compared the effects of direct current (DC, capacitive coupling (CC, pulsed electromagnetic field (PEMF and degenerate wave (DW on cellular activities including cytotoxicity, proliferation, cell-kinetics and apoptosis by stimulating human-BMMSCs 3 hours a day, up to 5 days. In addition, migration and invasion were assessed using fluorescence microscopy and by quantifying gene and protein expression. We found that DW had the greatest proliferative and least apoptotic and cytotoxic effects compared to other waveforms. DC, DW and CC stimulations resulted in a higher number of cells in S phase and G(2/M phase as shown by cell cycle analysis. CC and DW caused more cells to invade collagen and showed increased MMP-2 and MT1-MMP expression. DC increased cellular migration in a scratch-wound assay and all ES waveforms enhanced expression of migratory genes with DC having the greatest effect. All ES treated cells showed similar progenitor potential as determined by MSC differentiation assay. All above findings were shown to be statistically significant (p<0.05. We conclude that ES can influence BMMSCs activities, especially DW and CC, which show greater invasion and higher cell proliferation compared to other types of ES. Application of DW or CC to the fracture site may help in the recruitment of BMMSCs to the wound that may enhance rate of bone healing at the fracture site.

  1. The Theoretical, Methodological, and Practical Background for Looking at International Students' Learning Styles, Backgrounds, and Quality Perceptions with Regard to ASB's Three English-Language M.Sc. Programs

    DEFF Research Database (Denmark)

    Skaates, Maria Anne

    This paper looking at the methodological background for a questionnaire study of student perceptions with regard to own learning styles and backgrounds as well as the quality of education in Aarhus School of Business' 3 English-language M.Sc. programs (FIB, EU, and BPM). Theories and models about...... the internationalization of business schools, service quality, and student learning styles are discussed in the paper, as is the statistic methodology for treating the questionnaire responses of the respondents....

  2. The Theoretical, Methodological, and Practical Background for Looking at International Students' Learning Styles, Backgrounds, and Quality Perceptions with Regard to ASB's Three English-Language M.Sc. Programs

    OpenAIRE

    Skaates, Maria Anne

    2004-01-01

    This paper looking at the methodological background for a questionnaire study of student perceptions with regard to own learning styles and backgrounds as well as the quality of education in Aarhus School of Business' 3 English-language M.Sc. programs (FIB, EU, and BPM). Theories and models about the internationalization of business schools, service quality, and student learning styles are discussed in the paper, as is the statistic methodology for treating the questionnaire responses of the ...

  3. Near-infrared determination of reducing sugar content in honey based on multiplicative scatter correction partial least square(MSC/PLS)method%基于多元散射校正和偏最小二乘(MSC/PLS)的傅里叶变换近红外光谱检测蜂蜜中还原糖

    Institute of Scientific and Technical Information of China (English)

    张欣; 单杨; 李水芳

    2009-01-01

    为快速无损测定蜂蜜的还原糖,提出一种用近红外光谱技术进行检测的新方法.选用SN/T0852-2000铁氰化钾滴定法作为光谱还原糖测定的标定方法,用不同光谱预处理方法对模型的影响进行比较.首先用MSC对光谱数据进行校正,在经过Norris导数平滑法对原始光谱进行预处理消除噪声.利用光谱专用分析软件TQ Analyst7.2,建立偏最小二乘(PLS)校正模型.最优光谱模型校正相关系数(R)、校正相关均方差(RMSEC)、预测相关均方差(RMSEP)、交互验证相关系数(Rcv)、交叉验证均方差(REMSECV)分别为0.970 56,1.21,1.54,0.962 67,1.36.结果表明,应用多元散射校正(MSC)处理,并经过Norris二阶导数平滑后的PLS模型优于经典PLS算法所建立的模型.用MSC对光谱数据进行校正,在经过Norris导数平滑法对原始光谱进行预处理消除噪声.利用光谱专用分析软件TQ Analyst7.2,建立偏最小二乘(PLS)校正模型.最优光谱模型校正相关系数(R)、校正相关均方差(RMSEC)、预测相关均方差(RMSEP)、交互验证相关系数(Rcv)、交叉验证均方差(REMSECV)别为0.970 56,1.21,1.54,0.962 67,1.36.结果表明,应用多元散射校正(MSC)处理,

  4. Janis Huston Audin, MSc, DVM,1950-2009. Dynamic editor-in-chief of the Journal of the American Veterinary Medical Association and strong One Health advocate dies

    Directory of Open Access Journals (Sweden)

    Bruce Kaplan, DVM

    2009-09-01

    Full Text Available Dr Janis H. Audin (MSc Illinois 1975, DVM Illinois 1979, a champion of progressive veterinary medical journalism and ‘One Health’ died on 22 April 2009 following a long, courageous and difficult battle with pancreatic cancer. The world has lost a truly significant One Health leader and advocate. Under her guidance, the Journal of the American Veterinary Medical Association (JAVMA implemented a ‘one-health wonders’ column that recognised and highlighted prominent One Health individuals among the medical and veterinary medical professions in the United States. The American Veterinary Medical Association (AVMA has lost a dedicated and gifted editor-in-chief.Dr Audin joined the editorial staff of the AVMA in 1985, as an assistant editor and was promoted to associate editor in 1989 and editor in 1994. She became the editor-in-chief of both the JAVMA and the American Journal of Veterinary Research in 1995. Prior to that, Dr Audin practised as an associate veterinarian in Calumet City, Illinois, for four years.During her tenure, Dr Audin was noted for implementing procedural and technological changes in the journal to reduce costs, improve timeliness of publications and promote readership interest and awareness. New features in the News section introduced under her leadership have made the journals more practical and public health-relevant. For instance, Dr Audin fostered the United States Department of Agriculture’s Food Safety and Inspection Service (USDA-FSIS ‘Inspection Insights’ - a public health-oriented food safety monthly column related to meat, poultry and egg products - from 1996 through 1998. She also increased international manuscript submissions.On 23 March 2009 AVMA Executive Vice President Dr W. Ron DeHaven named Dr Audin as editor-in-chief emeritus of the Publications Division. Wisely, it also meant that Dr Audin could continue contributing to the staff effort to ensure the high quality of the AVMA scientific journals

  5. Püha Birgitta keskus : Merivälja tee 18, Tallinn / Ra Luhse

    Index Scriptorium Estoniae

    Luhse, Ra, 1964-

    2001-01-01

    Arhitektuur ja sisekujundus: Arhitektibüroo Luhse & Tuhal. Arhitektid Ra Luhse, Tanel Tuhal. Konstruktsioonid: Teinos OÜ. Ehitaja: AS FKSM. Projekt 1997-1999, klooster valmis 2001. 2 ill.: välisvaated

  6. Co-cultivation of keratinocyte-human mesenchymal stem cell (hMSC) on sericin loaded electrospun nanofibrous composite scaffold (cationic gelatin/hyaluronan/chondroitin sulfate) stimulates epithelial differentiation in hMSCs: In vitro study.

    Science.gov (United States)

    Bhowmick, Sirsendu; Scharnweber, Dieter; Koul, Veena

    2016-05-01

    Fortifying the scaffold with bioactive molecules and glycosaminoglycans (GAGs), is an efficient way to design new generation tissue engineered biomaterials. In this study, we evaluated the synergistic effect of electrospun nanofibrous composite scaffold (cationic gelatin/hyaluronan/chondroitin sulfate) loaded with sericin and, contact co-culture of human mesenchymal stem cells (hMSCs)-keratinocytes on hMSCs' differentiation towards epithelial lineage. Cationic gelatin is prepared with one step novel synthesis process by grafting quaternary ammonium salts to the backbone of gelatin. Release kinetics studies showed that Fickian diffusion is the major release mechanism for both GAGs and sericin/gelatin. In vitro biocompatibility of the electrospun scaffold was evaluated in terms of LDH and DNA quantification assay on human foreskin fibroblast, human keratinocyte and hMSC. Significant proliferation (∼ 4-6 fold) was detected after culturing all three cell on the electrospun scaffold containing sericin. After 5 days of contact co-culture, results revealed that electrospun scaffold containing sericin promote epithelial differentiation of hMSC in terms of several protein markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of some dermal proteins (keratin 14, ΔNp63α). Findings of this study will foster the progress of current skin tissue engineering scaffolds by understanding the skin regeneration and wound healing process. PMID:26946262

  7. 基于MSC.Fatigue的汽车横向稳定杆疲劳可靠性仿真及试验研究%MSG. Fatigue-based fatigue reliability simulation and experimental research of automotive stabilizer bar

    Institute of Scientific and Technical Information of China (English)

    黄康; 戚士兵; 叶鹏

    2012-01-01

    Based on the situation that the traditional fatigue reliability design of automotive stabilizer bar is rather conservative , which results in the lack of scientific basis in fatigue design, longer development period, cumbersome components , low fatigue reliability, etc. , this paper puts forward the combination method of fatigue reliability and fatigue finite element design. The finite element model of automotive stabilizer bar is built by the special fatigue analysis software MSC. Fatigue in the paper, and the minimum fatigue life and life distribution are calculated by finite element method in order to figure out the loading condition, dangerous position and fatigue life of the stabilizer bar. The fatigue test of the stabilizer bar shows that the estimated result agrees with the experimental one. It provides reference for the stabilizer bar's material selection, structure optimization and fatigue design.%基于传统汽车稳定杆疲劳可靠性设计偏于保守,造成疲劳设计缺乏科学依据、开发周期长、零件过于笨重、疲劳可靠性低等情况,提出将疲劳可靠性方法与疲劳有限元设计方法相结合,利用专业疲劳分析软件MSC.Fatigue,建立汽车稳定杆有限元模型,利用有限元方法计算其最小疲劳寿命和寿命分布,从而得到稳定杆的受力、危险部位、疲劳寿命等情况.稳定杆疲劳试验表明,预估结果与试验结果相吻合,为汽车稳定杆的材料选择、结构优化、疲劳设计提供了参考.

  8. Synthesis and characterization of aluminum and gallium complexes of heterocyclic thiosemicarbazones. crystal structures of Me2M[SC4H3CHNNC(S)SCH3] (M=Al, Ga)

    International Nuclear Information System (INIS)

    The synthesis and characterization of the monomeric group 13 heterocyclic thiosemicarbazone complexes Me2M[SC4H3CHNNC(S)SCH3] (M=Al (2), Ga (3)) are described. Compounds 2-3 were prepared using MMe3 (M=Al, Ga) in toluene with 2-thiophenecarboxaldehyde-S-methyldithiocarbazate under anaerobic conditions. These complexes have been characterized by 1H NMR, 13C NMR, elemental analyses, and single-crystal X-ray diffraction. 2 crystallizes in the monoclinic space group P21/c with unit cell parameters a=10.2930(5) A, b=18.564 (1) A, c=7.3812(6) A, V=1347.9(2) A3, Dcalc=1.342 gcm-3 for Z=4, 9281 reflections with Ibca with unit cell parameters a=13.340(3) A, b=19.9070(5) A, c=11.3690(2) A, V=2673.88(9) A3, Dcalc=1.511 gcm-3 for Z=8, 17004 reflections with I>3σ(I), R1=0.0480 and wR2=0.0524. Compound 3 is a monomeric gallium compound with a weak interaction between the pendant thiophene and the gallium center

  9. Cultuurverschillen in een internationale MSc opleiding

    NARCIS (Netherlands)

    Rip, F.I.

    2005-01-01

    De masteropleiding Geo-informatie wetenschappen aan Wageningen Universiteit kent een zeer diverse instroom van binnen- en buitenlandse studenten. Daarnaast is men bezig om de competentie-benadering in te voeren. In dit artikel wordt uitgelegd hoe één en ander samenhangt, met speciale aandacht voor c

  10. Research oriented MSc course on solar eruptions

    Science.gov (United States)

    Vainio, Rami; Heber, Bernd; Agueda, Neus; Kilpua, Emilia; Isavnin, Alexey; Afanasiev, Alexandr; Ganse, Urs; Koskinen, Hannu E. J.

    2014-05-01

    Department of Physics, University of Helsinki, organized a five-credit-point Master-level course on "Solar Eruptions and Space Environment" in spring 2013. The course, attended by nine students, included twenty hours of introductory lectures on solar eruptive phenomena (focusing on energetic particle emissions) as well as experimental and theoretical methods to analyze them. In addition, the course contained ten hours of exercise sessions, where solutions on short calculation exercises were presented and discussed. The main learning method on the course was, however, a coordinated scientific analysis of five solar eruptions observed by the STEREO spacecraft in 2010-2011. The students were grouped in four teams to study the solar eruptive events from four different view points: (1) Analysis of morphology and kinematics of coronal mass ejections, (2) analysis of EUV imaging observations of coronal wave-like transients, (3) solar and interplanetary magnetic field conditions during the eruptions, and (4) emission and transport modelling of near-relativistic electron events associated with the eruptions. Each group of students was assigned a scientist to oversee their work. The students reported weekly on their progress and gave a final presentation (of 30 minutes) in a seminar session at the end of the seven-week course. Grading of the course was based on the home exercises and final presentations. Students were also asked to give anonymous feedback on the course. Learning results on the course were very encouraging, showing that research oriented courses with practical research exercises on specific topics give students deeper knowledge and more practical skills than traditional lectures and home exercises alone.

  11. 全日制兽医专业硕士学位研究生培养体系初探%Preliminary Study on Cultivation System of Full-time Veterinary MSC Degree Graduates

    Institute of Scientific and Technical Information of China (English)

    郭定宗; 程国富; 毕丁仁; 周东海; 李家奎

    2011-01-01

    针对目前我国全日制兽医专业硕士学位研究生培养体系进行了初步研究,提出了全日制兽医硕士专业学位培养体系应以培养目标为中心,以改革课程设置,重点培养学生的实践能力、动手能力和分析问题能力、解决问题的能力为培养环节;以建立新型的实践教学考核评价体系为导向;以校企联合培养提高培养质量和扩大规模为有效途径;以实施导师、学校、学院3级分权管理体制等建议。%This paper studies the cultivation system of full-time veterinary MSC degree postgraduate based on the current situation and puts forward several relevant suggestions. These suggestions include. first, cultivation system of full-time veterinary Master's Degree should be centered around training objectives. The training process should focus on reforming curriculum, training students' practical ability, DIY ability and ability to analyze and solve problems. Second, a new practice-oriented teaching evaluation system should be established. Thirdly, it is the effective way to improve training quality through joint cultivation between colleges and enterprises and expand the scales. Finally, three-level decentralized management among instructors, schools and colleges should be implemented.

  12. The Immunomodulation Effect of UC-MSC on the Inflammatory Chemokines of Rats with Collagen Type Ⅱ Induced Arthritis%脐带间充质干细胞移植对胶原诱导型关节炎大鼠炎性趋化因子表达的影响

    Institute of Scientific and Technical Information of China (English)

    康丽莉; 于立耘; 倪军; 顾健; 林传明; 沈连军; 顾蔚; 孙幸; 闪丹

    2012-01-01

    目的 观察脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSC)移植对胶原诱导型关节炎(collagen typeⅡ-induced arthritis,CIA)大鼠模型血清中:血管细胞间黏附分子-1(VCAM-1)、基质细胞衍生因子-1(SDF-1)、内皮细胞生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)表达水平的影响,初步探讨UC-MSC移植对CIA大鼠炎性趋化因子的调节作用.方法 采用鸡Ⅱ型胶原加完全弗氏佐剂建立CIA大鼠模型,经大鼠尾静脉注射UC-MSC;以酶联免疫吸附试验(ELISA)检测各组大鼠干预前后血清VCAM-1、SDF-1、VEGF、TNF-α水平变化.结果 模型组血清VCAM-1、SDF-1、VEGF、TNF-α水平均明显高于同期空白对照组(P<0.05);经UC-MSC治疗组上述炎症因子水平逐渐降低,至第5周上述指标均接近空白对照组(P<0.05).结论 CIA大鼠存在免疫功能紊乱,其炎症趋化因子水平显著增高;UC-MSC能下调CIA大鼠炎症趋化因子水平,减轻机体免疫性炎症反应,促进疾病缓解,改善预后.%Objective To observe the immunomodulation effects of umbilical cord mesenchymal stem cells (UC-MSC) on the VCAM-1, SDF-1, VEGF, TNF-α of rats with collagen Ⅱ induced arthritis ( CIA). Methods First, The rats were immunized by intradermal injection of chickencollagen type Ⅱ emulisified with complete Freund's adjuvant (CFA). The swelling of foot ,hair color ,and action state of the rats were observed after injecting UC-MSC by caudal vein,the rats were scored with the arthritis index( AI) once a week. Then,The CD 4,CD 25 of peripheral blood lymphocytes and the CD 11b of peripheral blood neutrophil were detected by flow cytometry(FCM) ;The concentration of IL-17 in serum was detected by ELISA. Results Obvious swelling of the feet were found in the experiment group compared with norma group. ELISA the concentration of VCAM-1 ,SDF-1, VEGF,TNF-α in serum of the experiment group are higher than normal group (P <0. 05). After UC-MSC treatment

  13. In memoriam: Janis Huston Audin, MSc, DVM, 1950-2009. Dynamic editor-in-chief of the Journal of the American Veterinary Medical Association and strong One Health advocate dies.

    Science.gov (United States)

    Kaplan, Bruce

    2009-01-01

    Dr Janis H. Audin (MSc Illinois 1975, DVM Illinois 1979), a champion of progressive veterinary medical journalism and 'One Health' died on 22 April 2009 following a long, courageous and difficult battle with pancreatic cancer. The world has lost a truly significant One Health leader and advocate. Under her guidance, the Journal of the American Veterinary Medical Association (JAVMA) implemented a 'one-health wonders' column that recognised and highlighted prominent One Health individuals among the medical and veterinary medical professions in the United States. The American Veterinary Medical Association (AVMA) has lost a dedicated and gifted editor-in-chief. Dr Audin joined the editorial staff of the AVMA in 1985, as an assistant editor and was promoted to associate editor in 1989 and editor in 1994. She became the editor-in-chief of both the JAVMA and the American Journal of Veterinary Research in 1995. Prior to that, Dr Audin practised as an associate veterinarian in Calumet City, Illinois, for four years. During her tenure, Dr Audin was noted for implementing procedural and technological changes in the journal to reduce costs, improve timeliness of publications and promote readership interest and awareness. New features in the News section introduced under her leadership have made the journals more practical and public health-relevant. For instance, Dr Audin fostered the United States Department of Agriculture's Food Safety and Inspection Service (USDA-FSIS) 'Inspection Insights' - a public health-oriented food safety monthly column related to meat, poultry and egg products - from 1996 through 1998. She also increased international manuscript submissions. On 23 March 2009 AVMA Executive Vice President Dr W. Ron DeHaven named Dr Audin as editor-in-chief emeritus of the Publications Division. Wisely, it also meant that Dr Audin could continue contributing to the staff effort to ensure the high quality of the AVMA scientific journals while the Association began a

  14. Profiles in Leadership: Donald E. Francke, MSc, DSc (Hon).

    Science.gov (United States)

    Stevenson, James G; Beham, Rachel E; Weber, Robert J

    2013-10-01

    The Director's Forum series is designed to guide pharmacy leaders in establishing patient-centered services in hospitals and health systems. August 2013 marked the 50th anniversary of the publication of the Mirror to Hospital Pharmacy, the results of a federally funded comprehensive study of pharmacy services in the United States. The late Don E. Francke, MS, DSc, was the lead author of the Mirror and the principal investigator for the US Public Health Service grant W-45. To celebrate the anniversary of the Mirror, the Director's Forum is profiling the leadership styles of Drs. Latiolais and Francke. September's article highlighted Dr. Clifton J. Latiolais; this month's Director's Forum reviews Dr. Francke's biography and key career accomplishments, describes his leadership philosophy, and translates that philosophy to today's health care challenges. Don's influence on health system pharmacy serves as an example of effective leadership. This historical perspective provides directors of pharmacy a valuable leadership view as they develop strategies to enhance patient-centered pharmacy services. PMID:24421553

  15. MSc in Information and Library Management at Dublin Business School

    OpenAIRE

    O'Neill, Marie

    2015-01-01

    Dublin Business School is Ireland’s largest independent higher education college offering over 100 accredited courses in business, arts, law, psychology, IT and more to over 9,000 students. In November 2006, Dublin Business School identified a niche in the marketplace for a postgraduate programme in library studies delivered on a part-time evening basis. DBS was ideally positioned to offer this programme as it has highly experienced faculty in IT and Management and strong links to industry...

  16. Profiles in Leadership: Clifton J. Latiolais, MSc, DSc.

    Science.gov (United States)

    White, Sara; Godwin, Harold N; Weber, Robert J

    2013-09-01

    The Director's Forum series is designed to guide pharmacy leaders in establishing patient-centered services in hospitals and health systems. August 2013 marks the 50th anniversary of the publication of the Mirror to Hospital Pharmacy, which was a comprehensive study of pharmacy services in the United States. The late Clifton J. Latiolais, MS, DSc, served as the assistant program director for the study and was a co-author of the Mirror. The late Don E. Francke, MS, DSc, was the lead author of the Mirror and the principal investigator of the federally funded study that reviewed hospital pharmacy services across the United States. The next 2 articles in Director's Forum profile the leadership of Drs. Latiolais and Francke. This article highlights Dr. Latiolais ("Clif") by briefly reviewing his biography and key career accomplishments, describing his leadership philosophy, and translating that philosophy to today's health care challenges. Clif's influence on health system pharmacy serves as an example of effective leadership. This historical perspective on Clif's leadership, as seen through the eyes of those who knew him, provides directors of pharmacy a valuable leadership viewpoint as they develop strategies to enhance patient-centered pharmacy services.

  17. Organization of BSc and MSc projects in project families

    DEFF Research Database (Denmark)

    Ottosen, Lisbeth M.; Goltermann, Per; Jensen, Pernille Erland

    2014-01-01

    This work reports organization of student thesis projects in project families, with the benefit to both teaching and learning. The project organization went from student projects broadly distributed on topics related to different research issues and individual supervision to project families with...

  18. Measuring student performance in an international MSc programme

    DEFF Research Database (Denmark)

    Richelsen, Ann Bettina

    2011-01-01

    The European Bologna Process initiated by the Bologna Declaration has increased the student mobility. The Technical University of Denmark (DTU) offers two years international Master of Science in Engineering programmes, and the present work addresses the question whether there is a difference in ...... the student performance of international compared to Danish students in one of these programmes. The comparison is based on weighted grade average and course of study. The engineering education structure at DTU is explained and a number of results are discussed.......The European Bologna Process initiated by the Bologna Declaration has increased the student mobility. The Technical University of Denmark (DTU) offers two years international Master of Science in Engineering programmes, and the present work addresses the question whether there is a difference in...

  19. MULTIDISCIPLINARY TEACHING – MSc COURSE ON TEAMWORK AND OPARATION

    OpenAIRE

    Karlshøj, Jan; Dederichs, Anne

    2011-01-01

    Industrialization and technical development led to a split of the traditional role of the master builder into two: the architect and the engineer. Additionally, new demands on functionality such as energy and cost efficiency led to an increasing need of functioning collaboration in large teams during the design phase; as well as a need of new work methods within the process. This calls for employees who are experienced in collaborating in interdisciplinary teams. To fulfill this demand a mult...

  20. Piezoelectric Actuator Modeling Using MSC/NASTRAN and MATLAB

    Science.gov (United States)

    Reaves, Mercedes C.; Horta, Lucas G.

    2003-01-01

    This paper presents a procedure for modeling structures containing piezoelectric actuators using MSCMASTRAN and MATLAB. The paper describes the utility and functionality of one set of validated modeling tools. The tools described herein use MSCMASTRAN to model the structure with piezoelectric actuators and a thermally induced strain to model straining of the actuators due to an applied voltage field. MATLAB scripts are used to assemble the dynamic equations and to generate frequency response functions. The application of these tools is discussed using a cantilever aluminum beam with a surface mounted piezoelectric actuator as a sample problem. Software in the form of MSCINASTRAN DMAP input commands, MATLAB scripts, and a step-by-step procedure to solve the example problem are provided. Analysis results are generated in terms of frequency response functions from deflection and strain data as a function of input voltage to the actuator.

  1. Branka Barišić MSc in Technical Sciences

    OpenAIRE

    Vlado Cetl

    2012-01-01

    Branka Barišić defended her Master’s thesis Coastal Spatial Data Infrastructure at the Faculty of Geodesy of the University of Zagreb on July 13, 2012. Her mentor was Prof. Dr. Vlado Cetl. The examining and defence committee consisted of Prof. Dr. Siniša Mastelić Ivić, Prof. Dr. Miodrag Roić and Prof. Dr.Vlado Cetl.

  2. Field-ion microscopy, MSC report No. 4691

    International Nuclear Information System (INIS)

    This short article discusses the elements of a basic field ion microscope (FIM), the physics of the processes of field ionization and field evaporation, the factors limiting atomic resolution, the principle features of an atom probe FIM and imaging atom-probe mass spectroscopy

  3. MULTIDISCIPLINARY TEACHING – MSc COURSE ON TEAMWORK AND OPARATION

    DEFF Research Database (Denmark)

    Karlshøj, Jan; Dederichs, Anne

    2011-01-01

    of Denmark. The goal of the course is to provide training in teamwork at the final stage of the engineering education. The course has been carried out twice. It was held by a multidisciplinary team of professors in periods 2008/09, 2009/10 and 20010/2011. Teams of students were subject of a questionnaire...... investigation on collaboration and team work. The study has the following findings. The latest year there has been a special focus on team work and all members tested their role according to Belbin’s theory on teamwork. The work has the following findings: Collaboration was generally good. However the extra...... focus on teamwork did not lead to a improvement of the team work in contrary. The team-structure was generally flat and decisions were mostly made in consensus. It is worthwhile to offer a multidisciplinary course and give engineering students experience in collaboration methods....

  4. USA üritab Assange'i ja sõnavabadust risti lüüa / Birgitta Jónsdóttir ; intervjueerinud Askur Alas

    Index Scriptorium Estoniae

    Jónsdóttir, Birgitta

    2011-01-01

    Intervjuu Islandi parlamendisaadiku ja Wikileaksi endise kaastöötajaga USA nõudmisest, et Twitter annaks USA-le saadiku isiklikku infot, põhjusest, miks ta pole enam Wikileaksiga seotud. Ta usub, et samasuguseid internetisaite ja organisatsioone tekib veelgi

  5. Atomic site preferences and its effect on magnetic structure in the intermetallic borides M{sub 2}Fe(Ru{sub 0.8}T{sub 0.2}){sub 5}B{sub 2} (M=Sc, Ti, Zr; T=Ru, Rh, Ir)

    Energy Technology Data Exchange (ETDEWEB)

    Brgoch, Jakoah, E-mail: jrbrgoc@gmail.com [Department of Chemistry, Iowa State University, Ames, IA 50011 (United States); Mahmoud, Yassir A. [Department of Chemistry, Iowa State University, Ames, IA 50011 (United States); Miller, Gordon J., E-mail: gmiller@iastate.edu [Department of Chemistry, Iowa State University, Ames, IA 50011 (United States)

    2012-12-15

    The site preference for a class of intermetallic borides following the general formula M{sub 2}Fe(Ru{sub 0.8}T{sub 0.2}){sub 5}B{sub 2} (M=Sc, Ti, Zr; T=Ru, Rh, Ir), has been explored using ab initio and semi-empirical electronic structure calculations. This intermetallic boride series contains two potential sites, the Wyckoff 2c and 8j sites, for Rh or Ir to replace Ru atoms. Since the 8j site is a nearest neighbor to the magnetically active Fe atom, whereas the 2c site is a next nearest neighbor, the substitution pattern should play an important role in the magnetic structure of these compounds. The substitution preference is analyzed based on the site energy and bond energy terms, both of which arise from a tight-binding evaluation of the electronic band energy, and are known to influence the locations of atoms in extended solids. According to these calculations, the valence electron-rich Rh and Ir atoms prefer to occupy the 8j site, a result also corroborated by experimental evidence. Additionally, substitution of Rh or Ir at the 8j site results in a modification of the magnetic structure that ultimately results in larger local magnetic moment on the Fe atoms. - Graphical abstract: The site preference for electron rich atoms to occupy the 8j (gray) site is identified in these intermetallic borides, while the magnetic structure is modified as a function of the substituted atoms band center. Highlights: Black-Right-Pointing-Pointer We identify the energetics dictating the site preference in a series of intermetallic borides. Black-Right-Pointing-Pointer Establish substitution rules for use in future directed synthetic preparations. Black-Right-Pointing-Pointer Identified changes in magnetic structure that accompany the site preference.

  6. Täiuslik austusavaldus Maria Callasele / Tiiu Levald

    Index Scriptorium Estoniae

    Levald, Tiiu, 1940-

    2007-01-01

    17. augustil Moskva Novaja Opera interpreedid Dmitri Volosnikovi dirigeerimisel Pirita kloostris Birgitta festivali raames toimunud Maria Callase repertuaarist lavastatud galakontserdil "Maria Callas"

  7. Mõttekilde fantaasialennust suvelavadel / Tiiu Levald

    Index Scriptorium Estoniae

    Levald, Tiiu, 1940-

    2010-01-01

    Birgitta festivalil etendunud Glucki ooperist "Orpheus ja Eurydike", lavastaja Georg Rootering, R. Wagneri ooperist "Lohengrin", lavastaja Kaspar Holten ja G. Rossini ooperist "Sevilla habemeajaja", lavastaja Eliah Moshinsky

  8. Sympathetic Denervation-Induced MSC Mobilization in Distraction Osteogenesis Associates with Inhibition of MSC Migration and Osteogenesis by Norepinephrine/adrb3

    OpenAIRE

    Du, Zhaojie; Wang, Lei; Zhao, Yinghua; Cao, Jian; Tao WANG; Liu, Peng; Zhang, Yabo; Yang, Xinjie; Cheng, Xiaobing; Liu, Baolin; Lei, Delin

    2014-01-01

    The sympathetic nervous system regulates bone formation and resorption under physiological conditions. However, it is still unclear how the sympathetic nerves affect stem cell migration and differentiation in bone regeneration. Distraction osteogenesis is an ideal model of bone regeneration due to its special nature as a self-engineering tissue. In this study, a rat model of mandibular distraction osteogenesis with transection of cervical sympathetic trunk was used to demonstrate that sympath...

  9. Жаркое и десерт на фестивале Birgitta / Айвар Лоог

    Index Scriptorium Estoniae

    Лоог, Айвар

    2010-01-01

    Richard Wagneri "Lohengrin", lavastaja Kasper Holten ja Gioachino Rossini "Sevilla habemeajaja", lavastaja Elijah Moshinsky. Mõlemad Moskva "Новая Опера" lavastused (The Kolobov Novaya Opera Theatre)

  10. Dimensionality and spreading influence MSC YAP/TAZ signaling in hydrogel environments.

    Science.gov (United States)

    Caliari, Steven R; Vega, Sebastián L; Kwon, Michelle; Soulas, Elizabeth M; Burdick, Jason A

    2016-10-01

    Improved fundamental understanding of how cells interpret microenvironmental signals is integral to designing better biomaterial therapies. YAP/TAZ are key mediators of mechanosensitive signaling; however, it is not clear how they are regulated by the complex interplay of microenvironmental factors (e.g., stiffness and degradability) and culture dimensionality. Using covalently crosslinked norbornene-functionalized hyaluronic acid (HA) hydrogels with controlled stiffness (via crosslink density) and degradability (via susceptibility of crosslinks to proteolysis), we found that human mesenchymal stem cells (MSCs) displayed increased spreading and YAP/TAZ nuclear localization when cultured atop stiffer hydrogels; however, the opposite trend was observed when MSCs were encapsulated within degradable hydrogels. When stiffness-matched hydrogels of reduced degradability were used, YAP/TAZ nuclear translocation was greater in cells that were able to spread, which was confirmed through pharmacological inhibition of YAP/TAZ and actin polymerization. Together, these data illustrate that YAP/TAZ signaling is responsive to hydrogel stiffness and degradability, but the outcome is dependent on the dimensionality of cell-biomaterial interactions. PMID:27429252

  11. Dissertation presentation in part consideration for MSc. in Supply Chain and Operations Management

    OpenAIRE

    Olufeko, Olajumoke T.

    2011-01-01

    Foreign direct investment (FDI) is what most countries strive to attract as it is an advantageous tool for economic development. Nigeria not being left out also joined the rest of the world in seeking FDI as shown by the formation of the New Partnership for Africa’s Development(NEPAD, which is responsible for attracting FDI to Africa as a major component. This study looks into the impact of foreign direct investment on Nigerian economy between 1991 and 2010. The study was carried out...

  12. Mineralized collagen scaffolds induce hMSC osteogenesis and matrix remodeling

    OpenAIRE

    Weisgerber, D.W.; Caliari, S.R.; Harley, B.A.C.

    2015-01-01

    Biomaterials for bone tissue engineering must be able to instruct cell behavior in the presence of the complex biophysical and biomolecular environments encountered in vivo. While soluble supplementation strategies have been identified to enhance osteogenesis, they are subject to significant diffusive loss in vivo or the need for frequent re-addition in vitro. This investigation therefore explored whether biophysical and biochemical properties of a mineralized collagen-GAG scaffold were suffi...

  13. Photogrammetry and Remote Sensing in the MSc Program "ESPACE" at Technische Universitaet Muenchen

    Science.gov (United States)

    Stilla, U.; Iwaszczuk, D.; Pail, R.

    2014-04-01

    ESPACE is an interdisciplinary Master's study programme of Technische Universitaet Muenchen (TUM) positioned at the interface between space technology and the engineering and natural science-based use of satellite data. It combines the technical aspects of the satellite and observation systems with scientific and commercial applications. A core topic beside space engineering and satellite applications engineering is satellite and remote sensing data analysis. This paper explains the background and goals of the Masters's study programme, addresses the target groups and qualification profile, and gives an overview about the specialization fields. The special role of Photogrammetry and Remote Sensing is further explained in this context. The structure and embedding of international alliances by a double degree program with Wuhan University is shown.

  14. Quantification of Mesenchymal Stem Cell (MSC delivery to a target site using in vivo confocal microscopy.

    Directory of Open Access Journals (Sweden)

    Luke J Mortensen

    Full Text Available The ability to deliver cells to appropriate target tissues is a prerequisite for successful cell-based therapy. To optimize cell therapy it is therefore necessary to develop a robust method of in vivo cell delivery quantification. Here we examine Mesenchymal Stem Cells (MSCs labeled with a series of 4 membrane dyes from which we select the optimal dye combination for pair-wise comparisons of delivery to inflamed tissue in the mouse ear using confocal fluorescence imaging. The use of an optimized dye pair for simultaneous tracking of two cell populations in the same animal enables quantification of a test population that is referenced to an internal control population, thereby eliminating intra-subject variations and variations in injected cell numbers. Consistent results were obtained even when the administered cell number varied by more than an order of magnitude, demonstrating an ability to neutralize one of the largest sources of in vivo experimental error and to greatly reduce the number of cells required to evaluate cell delivery. With this method, we are able to show a small but significant increase in the delivery of cytokine pre-treated MSCs (TNF-α & IFN-γ compared to control MSCs. Our results suggest future directions for screening cell strategies using our in vivo cell delivery assay, which may be useful to develop methods to maximize cell therapeutic potential.

  15. Adult Cardiac-Resident MSC-like Stem Cells with a Proepicardial Origin

    NARCIS (Netherlands)

    Chong, James J. H.; Chandrakanthan, Vashe; Xaymardan, Munira; Asli, Naisana S.; Li, Joan; Ahmed, Ishtiaq; Heffernan, Corey; Menon, Mary K.; Scarlett, Christopher J.; Rashidianfar, Amirsalar; Biben, Christine; Zoellner, Hans; Colvin, Emily K.; Pimanda, John E.; Biankin, Andrew V.; Zhou, Bin; Pu, William T.; Prall, Owen W. J.; Harvey, Richard P.

    2011-01-01

    Colony-forming units fibroblast (CFU-Fs), analogous to those giving rise to bone marrow (BM) mesenchymal stem cells (MSCs), are present in many organs, although the relationship between BM and organ-specific CFU-Fs in homeostasis and tissue repair is unknown. Here we describe a population of adult c

  16. Formal methods impact on ANSI standard HL7/IM: filling gaps in MSC theory

    NARCIS (Netherlands)

    Wesselink, W.; Goga, N.; Mooij, A.J.; Spronk, R.

    2005-01-01

    Health level seven (HL7) is an ANSI standard that provides a comprehensive framework for electronic health information. The most-widely used HL7 specification is called infrastructure management, which facilitates health-care applications to exchange key sets of clinical and administrative data. Thi

  17. Study of light-induced MscL gating by EPR spectroscopy

    NARCIS (Netherlands)

    Yilmaz, Duygu; Dimitrova, Anna I.; Walko, Martin; Kocer, Armagan

    2015-01-01

    A number of techniques developed to investigate protein structure and function depend on chemically modifying and/or labeling of proteins. However, in the case of homooligomeric proteins, the presence of multiple identical subunits obstructs the introduction of residue-specific labels to only one or

  18. Upgrading and extended testing of the MSC integrated water and waste management hardware

    Science.gov (United States)

    Bambenek, R. A.; Nuccio, P. P.; Hurley, T. L.; Jasionowski, W. J.

    1972-01-01

    The results are presented of upgrading and testing an integrated water and waste management system, which uses the compression distillation, reverse osmosis, adsorption filtration and ion-exchange processes to recover potable water from urine, flush water and used wash water. Also included is the development of techniques for extending the useful biological life of biological filters, activated carbon filters and ion-exchange resins to at least 30 days, and presterilizing ion-exchange resins so that sterile water can be recovered from waste water. A wide variety of reverse osmosos materials, surfactants and germicides were experimentally evaluated to determine the best combination for a wash water subsystem. Full-scale module tests with real wash water demonstrated that surface fouling is a major problem.

  19. A new MSc course on diagnostics of electrical machines and power electronics

    DEFF Research Database (Denmark)

    Leban, Krisztina Monika; Ritchie, Ewen

    2011-01-01

    students. Additionally, specific subjects requested by participants, basic diagnosis and testing methods were presented during the lectures and workshops. General engineering knowledge about electric machines, power electronics and the combination of these was presented. The laboratory method, experiments...

  20. Polycaprolactone nanomesh cultured with hMSC evaluated by synchrotron tomography

    DEFF Research Database (Denmark)

    Nygaard, Jens Vinge; Andersen, Morten Østergaard; Cloetens, Peter;

    substrates while proliferating preferentially on the stiffer ones. This implicates that substrate rigidity is a critical design parameter in the development of scaffolds aimed at eliciting maximal cell and tissue function. From mechanics it is known that the stiffness of a porous structures scales...... network. These beams are called Plateau borders and are typically solid structures. Thus their stiffness depends solely on the stiffness of the selected biopolymer and the method of production. In this study we demonstrate that it is possible to control the porosity not only of the macroscopic porous...... scaffold but also of the Plateau borders constituting the scaffold.   Materials and Methods Polycaprolactone scaffolds were prepared by thermal induced phase separation followed by lyophilization. Processing conditions were chosen to range the relative density of the obtained scaffolds and its Plateau...

  1. Polycaprolactone nanomesh cultured with hMSC evaluated by synchrotron tomography

    DEFF Research Database (Denmark)

    Nygaard, Jens Vinge; Andersen, Morten Østergaard; Cloetens, Peter;

    2009-01-01

    substrates while proliferating preferentially on the stiffer ones. This implicates that substrate rigidity is a critical design parameter in the development of scaffolds aimed at eliciting maximal cell and tissue function. From mechanics it is known that the stiffness of a porous structures scales...... network. These beams are called Plateau borders and are typically solid structures. Thus their stiffness depends solely on the stiffness of the selected biopolymer and the method of production. In this study we demonstrate that it is possible to control the porosity not only of the macroscopic porous...... scaffold but also of the Plateau borders constituting the scaffold.   Materials and Methods Polycaprolactone scaffolds were prepared by thermal induced phase separation followed by lyophilization. Processing conditions were chosen to range the relative density of the obtained scaffolds and its Plateau...

  2. Power Electronics Design Laboratory Exercise for Final-Year M.Sc. Students

    Science.gov (United States)

    Max, L.; Thiringer, T.; Undeland, T.; Karlsson, R.

    2009-01-01

    This paper presents experiences and results from a project task in power electronics for students at Chalmers University of Technology, Goteborg, Sweden, based on a flyback test board. The board is used in the course Power Electronic Devices and Applications. In the project task, the students design snubber circuits, improve the control of the…

  3. An (even) broader perspective: Combining environmental processes and natural hazards education in a MSc programme

    Science.gov (United States)

    Heckmann, Tobias; Haas, Florian; Trappe, Martin; Cyffka, Bernd; Becht, Michael

    2010-05-01

    Natural hazards are processes occurring in the natural environment that negatively affect human society. In most instances, the definition of natural hazards implies sudden events as different as earthquakes, floods or landslides. In addition, there are other phenomena that occur more subtly or slowly, and nevertheless may have serious adverse effects on the human environment. Hence, a comprehensive study programme in natural hazards has to include not only the conspicuous causes and effects of natural catastrophes, but of environmental processes in general. Geography as a discipline is located at the interface of natural, social and economic sciences; the physical geography programme described here is designed to include the social and economic dimension as well as management issues. Modules strengthening the theoretical background of geomorphic, geological, hydrological and meteorological processes and hazards are complemented by practical work in the field and the laboratory, dealing with measuring and monitoring environmental processes. On this basis, modeling and managing skills are developed. Another thread in the transdisciplinary programme deals with sustainability and environmental policy issues, and environmental psychology (e.g. perception of and reaction to hazards). This will improve the communication and team working skills of students wherever they are part of an interdisciplinary working group. Through the involvement in research programmes, students are confronted ‘hands on' with the different aspects of environmental processes and their consequences; thus, they will be excellently but not exclusively qualified for positions in the ‘natural hazards' sector.

  4. Inimhääle lummus ja topeltverism / Tiiu Levald

    Index Scriptorium Estoniae

    Levald, Tiiu, 1940-

    2008-01-01

    Birgitta festivali raames 10. ja 11. VIII Pirita kloostris toimunud etendustest - Mascagni "Talupoja au", Leoncavallo "Pajatsid" ja Donizetti "Maria Stuart" Moskva Novaja Opera esituses, dirigendid: Eri Klas, Valeri Kritskov ja Sergei Lõssenko

  5. Mozarti spirituaalne sadomaso / Alvar Loog

    Index Scriptorium Estoniae

    Loog, Alvar, 1975-

    2006-01-01

    11.-20. augustini Birgitta festivali raames Pirita kloostri varemetes etendunud Mozarti ooperist "Tituse halastus" (Moskva Helikoni teatri esituses), Tallinna raekojas etendunud Mozarti intermezzo-komöödiast "Apollo ja Hyacinthus"

  6. Keerukas terminal valiti aasta betoonehitiseks / Aivo Vahemets

    Index Scriptorium Estoniae

    Vahemets, Aivo

    2002-01-01

    Aasta Betoonehitise nimetuse võitis Muuga kuivpuisteainete terminal, projekteerija Randväli&Karema AS. Eriauhind arhitektuurse osa eest - Birgitta klooster Pirital. Eriauhind tellijale - büroohoone Tallinnas Toompuiestee 33. Eriauhind ehitajale - konteinerterminal Muugal, projekteerija AS Merin

  7. Photos of the conference

    Directory of Open Access Journals (Sweden)

    Birgitta Åhman

    1984-05-01

    Full Text Available Birgitta  Åhman is the photographer of the series of pictures from the conference, also for the cover photo of the full paper edition showing Kongsvold Mountain Hut and Biological Station.

  8. Amor meus, Cruxifixus est! / Teresa Perciaccante ; tõlk. Anne Kalling

    Index Scriptorium Estoniae

    Perciaccante, Teresa

    2002-01-01

    Kevadisest kriisist Petlemmas, mil Iisraeli armee piiras Jeesus Kristuse sünnikirikut. Ema Teresa Perciaccante kiri Pühima Päästja Püha Birgitta ordu ülemaabtissile Ema Tekla Famigliettile Rooma

  9. Pikk tee koos Pipiga / Herdis Kirk

    Index Scriptorium Estoniae

    Kirk, Herdis

    2003-01-01

    Vanemuise teatri näitlejast H. Merzinist, kes mängib Rootsis Värnamos peaosa illustraator I. Wiklandi autobiograafilisel teosel põhineva teose lavastuses "Pikk-pikk teekond", lavastaja Birgitta Englin

  10. 基于MSC Nastran的无人机复合材料机翼有限元分析%FINITE ELEMENT ANALYSIS OF COMPOSITE WING FOR UNMANNED AERIAL VEHICLE BASED ON MSC.NASTRAN

    Institute of Scientific and Technical Information of China (English)

    尹星研; 冯振宇; 卢翔

    2010-01-01

    以某型无人机复合材料机翼为基础,基于MCS.Patran建立四种不同结构机翼有限元模型,并利用MCS.Nastran进行强度校核和模态分析.分析各元件的承载情况,对机翼的结构进行优化,从而提高机翼的寿命,降低生产成本.

  11. Protein Localization in Escherichia coli Cells: Comparison of the Cytoplasmic Membrane Proteins ProP, LacY, ProW, AqpZ, MscS, and MscL

    OpenAIRE

    Romantsov, Tatyana; Battle, Andrew R.; Hendel, Jenifer L.; Martinac, Boris; Wood, Janet M.

    2010-01-01

    Fluorescence microscopy has revealed that the phospholipid cardiolipin (CL) and FlAsH-labeled transporters ProP and LacY are concentrated at the poles of Escherichia coli cells. The proportion of CL among E. coli phospholipids can be varied in vivo as it is decreased by cls mutations and it increases with the osmolality of the growth medium. In this report we compare the localization of CL, ProP, and LacY with that of other cytoplasmic membrane proteins. The proportion of cells in which FlAsH...

  12. MScMS-II: an innovative IR-based indoor coordinate measuring system for large-scale metrology applications

    OpenAIRE

    Pralio, Barbara; Galetto, Maurizio; Mastrogiacomo, Luca

    2011-01-01

    According to the current great interest concerning large-scale metrology applications in many different fields of manufacturing industry, technologies and techniques for dimensional measurement have recently shown a substantial improvement. Ease-of-use, logistic and economic issues, as well as metrological performance are assuming a more and more important role among system requirements. This paper describes the architecture and the working principles of a novel infrared (IR) optical-based sy...

  13. Negotiating and Designing Public Space. Experiences with a new M.Sc. in Urban Design Program in Hong Kong

    Directory of Open Access Journals (Sweden)

    Hendrik Tieben

    2013-05-01

    Full Text Available This contribution reflects on first experiences made with a newly launched Master of Science in Urban Design program at the Chinese University of Hong Kong. As an important part of this program, students have to develop their design proposal in response to feedback of different stakeholders and community members. Thus the program responds to the growing aspiration of Hong Kong’s citizens to shape the urban development of their city and a lack of a meaningful participation process in the region. With its high density, protected country parks, efficient public transport and large scale housing program, generally, Hong Kong offers important lessons for contemporary urbanism. However, since the end of the British colonial rule and in face of increasing property prices, pollution and the disappearance of local heritage, intensive debates started about the regions future. Another central point of the recent discussion in Hong Kong – and key theme of the new urban design program - is the demand for the rights and qualities of public space. The paper presents the set-up of the design studio, which was closely linked to a course on “urban processes”. During the semester, students had to organize community forums and street exhibitions in a specific district, invite stakeholders and residents and discuss with them their ideas. Their projects, then, had to respond on the various feedbacks and integrate them in their design and policy proposals. The text reflects on the student projects and the lessons learned in the process. It addresses general questions such as the challenges in communicating with a diverse community (e.g. language barriers and culturally different ideas of public space. It addresses the question of the intended and unintended effects of a participatory design studio in the community, and possible follow-ups. And it reflects on the general role of design and designers in shaping community spaces.

  14. A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration

    Science.gov (United States)

    Meng, Qingyang; Man, Zhentao; Dai, Linghui; Huang, Hongjie; Zhang, Xin; Hu, Xiaoqing; Shao, Zhenxing; Zhu, Jingxian; Zhang, Jiying; Fu, Xin; Duan, Xiaoning; Ao, Yingfang

    2015-12-01

    Articular cartilage injury is still a significant challenge because of the poor intrinsic healing potential of cartilage. Stem cell-based tissue engineering is a promising technique for cartilage repair. As cartilage defects are usually irregular in clinical settings, scaffolds with moldability that can fill any shape of cartilage defects and closely integrate with the host cartilage are desirable. In this study, we constructed a composite scaffold combining mesenchymal stem cells (MSCs) E7 affinity peptide-modified demineralized bone matrix (DBM) particles and chitosan (CS) hydrogel for cartilage engineering. This solid-supported composite scaffold exhibited appropriate porosity, which provided a 3D microenvironment that supports cell adhesion and proliferation. Cell proliferation and DNA content analysis indicated that the DBM-E7/CS scaffold promoted better rat bone marrow-derived MSCs (BMMSCs) survival than the CS or DBM/CS groups. Meanwhile, the DBM-E7/CS scaffold increased matrix production and improved chondrogenic differentiation ability of BMMSCs in vitro. Furthermore, after implantation in vivo for four weeks, compared to those in control groups, the regenerated issue in the DBM-E7/CS group exhibited translucent and superior cartilage-like structures, as indicated by gross observation, histological examination, and assessment of matrix staining. Overall, the functional composite scaffold of DBM-E7/CS is a promising option for repairing irregularly shaped cartilage defects.

  15. Requalification of offshore structures. Summaries of 9{sup TH} semester and M.SC projects on offshore structural reliability

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-07-01

    This project discusses the determination of the structural safety of an existing and reinforced offshore structure in the North Sea. As subject the example platform used in EFP-97 project 'Requalification of Offshore Structures' is chosen. A reliability analysis of the platform is performed; the reliability analysis includes an extreme-, fatigue- and system analysis. The reliability analysis is based on a stochastic model including both loading and structural strength. The platform is modeled in a 3D non-linear finite element program RONJA. These models are used to determine the structural safety by approximating the failure function with a quadretic response surface. The safety of the platform is evaluated for different levels of subsidence, and a critical subsidence level is determined. Given the subsidence level different methods of reinforcement is analyzed. The reinforcement methods includes insertion of new members and grouting. A fatigue analysis is performed for two critical joints. A 3D finite element model is constructed in ABAQUS and used to determine the stress concentration factors in the joints. The fatigue lives of the two joints are estimated by use of a deterministic and a probabilistic approach. The overall safety of the reinforced platform is determined by a systems reliability analysis including both fatique and extreme analyses. All analyses indicate an improvement of the structural safety after reinforcement of the structure. (au)

  16. Education for health: case studies of two multidisciplinary MPH/MSc public health programmes in the UK.

    Science.gov (United States)

    El Ansari, W; Russell, J; Wills, J

    2003-09-01

    Amidst the winds of change that are blowing across the UK public health (PH) landscape in relation to the essential abilities and national standards that are required for the 'art and science' of PH, the preparation for a new cadre of 'PH professionals' is already underway. Several postgraduate masters programmes in public health (MPH) have taken on board the challenge of addressing the requisite sets of skills and expertise as a guide to their content and delivery. Although there are recommendations regarding teaching PH to undergraduate medical students, little consensus seems to exist on teaching postgraduate PH to non-medically qualified professionals, health managers and administrators. Employing a case study approach, this article analyses the methods used, philosophies and processes, structure and organization, outcomes to date, and lessons learnt from MPH programmes implemented at two institutions in the UK. The programmes have been initiated recently, and have had the opportunity to take on board the recent national guidelines about training standards. The findings indicate that preparatory work of the programmes, and the challenges and strengths in meeting the recent policy developments in PH training are pertinent points. The MPH programmes highlight key issues in interprofessional education and its purpose, its process and its outcomes in relation to multidisciplinary specialist practice. These programmes provide a variety of models for others wishing to develop or restructure their postgraduate PH teaching programmes. The finalization of the national standards for specialist practice in PH in the UK is encouraged, along with clearer working definitions of the domains of expertise required. Collectively, attention to these measures can ensure that the processes which teaching programmes embrace to refine their content and delivery will equip tomorrow's professionals with PH knowledge and skills.

  17. A study of institutional complexity and contractors' safety management strategies / y Chuanjing Ju (BEng., Msc, Chongqing University)

    OpenAIRE

    Ju, Chuanjing; 鞠傳靜

    2015-01-01

    While prior studies have examined the influence of environmental factors (e.g. regulatory, market, and socio-cultural environment) on construction safety accidents, the explanations offered remain piecemeal and the environmental factors are largely treated as given and immutable. In reality, the external environment is dynamic and continuously evolving. Contractors are subject to regulatory, market and socio-cultural segments of environment simultaneously. However, there has to date been litt...

  18. The influence of hyaluronic acid hydrogel crosslinking density and macromolecular diffusivity on human MSC chondrogenesis and hypertrophy.

    Science.gov (United States)

    Bian, Liming; Hou, Chieh; Tous, Elena; Rai, Reena; Mauck, Robert L; Burdick, Jason A

    2013-01-01

    Hyaluronic acid (HA) hydrogels formed via photocrosslinking provide stable 3D hydrogel environments that support the chondrogenesis of mesenchymal stem cells (MSCs). Crosslinking density has a significant impact on the physical properties of hydrogels, including their mechanical stiffness and macromolecular diffusivity. Variations in the HA hydrogel crosslinking density can be obtained by either changes in the HA macromer concentration (1, 3, or 5% w/v at 15 min exposure) or the extent of reaction through light exposure time (5% w/v at 5, 10, or 15 min). In this work, increased crosslinking by either method resulted in an overall decrease in cartilage matrix content and more restricted matrix distribution. Increased crosslinking also promoted hypertrophic differentiation of the chondrogenically induced MSCs, resulting in more matrix calcification in vitro. For example, type X collagen expression in the high crosslinking density 5% 15 min group was ~156 and 285% higher when compared to the low crosslinking density 1% 15 min and 5% 5 min groups on day 42, respectively. Supplementation with inhibitors of the small GTPase pathway involved in cytoskeletal tension or myosin II had no effect on hypertrophic differentiation and matrix calcification, indicating that the differential response is unlikely to be related to force-sensing mechanotransduction mechanisms. When implanted subcutaneously in nude mice, higher crosslinking density again resulted in reduced cartilage matrix content, restricted matrix distribution, and increased matrix calcification. This study demonstrates that hydrogel properties mediated through alterations in crosslinking density must be considered in the context of the hypertrophic differentiation of chondrogenically induced MSCs. PMID:23084553

  19. The influence of hyaluronic acid hydrogel crosslinking density and macromolecular diffusivity on human MSC chondrogenesis and hypertrophy

    OpenAIRE

    Bian, Liming; Hou, Chieh; Tous, Elena; Rai, Reena; Mauck, Robert L.; Burdick, Jason A.

    2012-01-01

    Hyaluronic acid (HA) hydrogels formed via photocrosslinking provide stable 3D hydrogel environments that support the chondrogenesis of mesenchymal stem cells (MSCs). Crosslinking density has a significant impact on the physical properties of hydrogels, including their mechanical stiffness and macromolecular diffusivity. Variations in the HA hydrogel crosslinking density can be obtained by either changes in the HA macromer concentration (1, 3, or 5% w/v at 15 min exposure) or the extent of rea...

  20. Deep tissue single cell MSC ablation using a fiber laser source to evaluate therapeutic potential in osteogenesis imperfecta

    Science.gov (United States)

    Tehrani, Kayvan F.; Pendleton, Emily G.; Lin, Charles P.; Mortensen, Luke J.

    2016-04-01

    Osteogenesis imperfecta (OI) is a currently uncurable disease where a mutation in collagen type I yields brittle bones. One potential therapy is transplantation of mesenchymal stem cells (MSCs), but controlling and enhancing transplanted cell survival has proven challenging. Therefore, we use a 2- photon imaging system to study individual transplanted cells in the living bone marrow. We ablated cells deep in the bone marrow and observed minimal collateral damage to surrounding tissue. Future work will evaluate the local impact of transplanted MSCs on bone deposition in vivo.

  1. Pure titanium particle loaded nanocomposites: study on the polymer/filler interface and hMSC biocompatibility.

    Science.gov (United States)

    Avolio, Roberto; D'Albore, Marietta; Guarino, Vincenzo; Gentile, Gennaro; Cocca, Maria Cristina; Zeppetelli, Stefania; Errico, Maria Emanuela; Avella, Maurizio; Ambrosio, Luigi

    2016-10-01

    The integration of inorganic nanoparticles into polymer matrices allows for the modification of physical properties as well as the implementation of new features for unexplored application fields. Here, we propose the study of a new metal/polymer nanocomposite fabricated by dispersing pure Ti nanoparticles into a poly(methylmetacrilate) matrix via solvent casting process, to investigate its potential use as new biomaterial for biomedical applications. We demonstrated that Ti nanoparticles embedded in the poly(methylmetacrilate) matrix can act as reinforcing agent, not negatively influencing the biological response of human mesenchymal stem cell in terms of cytotoxicity and cell viability. As a function of relative amount and surface treatment, Ti nanoparticles may enhance mechanical strength of the composite-ranging from 31.1 ± 2.5 to 43.7 ± 0.7 MPa-also contributing to biological response in terms of adhesion and proliferation mechanisms. In particular, for 1 wt% Ti, treated Ti nanoparticles improve cell materials recognition, as confirmed by higher cell spreading-quantified in terms of cell area via image analysis-locally promoting stronger interactions at cell matrix interface. At this stage, these preliminary results suggest a promising use of pure Ti nanoparticles as filler in polymer composites for biomedical applications. PMID:27585912

  2. Problems of internal acoustics in two and three dimensional cavities with deformable walls using the MSC/Nastran code

    Science.gov (United States)

    Derosa, S.; Cavaliere, M.

    1989-02-01

    A finite element computer code is used to examine the acoustic fields in typical aircraft cabins. Nastran and Patran codes are used because of their versatility and worldwide utilization. Experimental results and comparison with analytical methods are presented. The interactions between the vibrating structure and the induced pressure field in the cavity fluid are demonstrated.

  3. Degenerate Wave and Capacitive Coupling Increase Human MSC Invasion and Proliferation While Reducing Cytotoxicity in an In Vitro Wound Healing Model

    OpenAIRE

    Michelle Griffin; Syed Amir Iqbal; Anil Sebastian; James Colthurst; Ardeshir Bayat

    2011-01-01

    Non-unions pose complications in fracture management that can be treated using electrical stimulation (ES). Bone marrow mesenchymal stem cells (BMMSCs) are essential in fracture healing; however, the effect of different clinical ES waveforms on BMMSCs cellular activities remains unknown. We compared the effects of direct current (DC), capacitive coupling (CC), pulsed electromagnetic field (PEMF) and degenerate wave (DW) on cellular activities including cytotoxicity, proliferation, cell-kineti...

  4. Traffic analysis of existing traffic in Kulyab region in order to plan and configure a new GSM MSC for this region

    OpenAIRE

    Frostne, Isabel

    2011-01-01

    Wide area cellular mobile networks have rapidly evolved over the years. In the beginning achieving wide area coverage was a great achievement – enabling subscribers to call from wherever they were currently located and whenever they wanted. Additionally these systems supported mobility of subscribers, so that calls could continue even while a subscriber moved from one cell to another. Today mobility management is something everyone takes for granted. New functionality is continuously being de...

  5. Evaluation of AD-MSC (adipose-derived mesenchymal stem cells) as a vehicle for IFN-β delivery in experimental autoimmune encephalomyelitis.

    Science.gov (United States)

    Mohammadzadeh, Adel; Pourfathollah, Ali Akbar; Shahrokhi, Somayeh; Fallah, Ali; Tahoori, Mohammad Taher; Amari, Afshin; Forouzandeh, Mahdi; Soleimani, Masoud

    2016-08-01

    Interferon-β (IFN-β) is commonly used as a disease modifying drug for the treatment of relapse-remitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-β mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified adipose-derived mesenchymal stem cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-β) on the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Lymph node mononuclear cells and serum were examined by using RT-PCR and ELISA methods to measure the production of IL-10 and IL-17 gene and protein expression, respectively. Our results indicated that in the MSCs-VP/IFN-β treated group induction of Tregs and IL-10 and reduction of IL-17 were significant. Taken together, we showed that using AD-MSCs expressing IFN-β as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in EAE conceivably will improve the valuable effects of IFN-β in this autoimmune disease. PMID:27373971

  6. Atomic-resolution study of homogeneous radiation-induced precipitation in a neutron-irradiated W-10 at. % Re alloy. MSC report No. 5014

    International Nuclear Information System (INIS)

    The phenomenon of radiation-induced precipitation has been investigated in a W-10 at. % Re alloy using the atom-probe field-ion microscope. Results show a significant alteration of the microstructure of this alloy as a result of the fast-neutron irradiation. Precipitates with the composition approx. WRe (sigma phase) were detected at a density of 1016 cm-3. Coherent, semicoherent and possibly incoherent precipitates of the sigma phase have been observed. They were not associated with either linear or planar defects, or with any impurity atoms; i.e. a true homogeneous radiation-induced precipitation occurs in this alloy. A physical argument is presented for the nucleation of the sigma phase precipitates in the vicinity of displacement cascades produced by primary knock-on atoms. It is suggested that the nucleation of the sigma phase is due to the formation of tightly-bound mobil mixed dumbells which react to form an immobile rhenium cluster. The growth of this cluster into a precipitate is most likely driven by the irreversible vacancy: self-interstitial atom (SIA) annihilation reaction, as suggested recently by Cauvin and Martin. A mechanism for the suppression of voids, in this alloy, is presented which is self-consistent with the homogeneous radiation-induced precipitation mechanism

  7. Comparison of microCT and an inverse finite element approach for biomechanical analysis: Results in a MSC therapeutic system for fracture healing

    OpenAIRE

    Weis, Jared A.; Granero-Moltó, Froilán; MYERS, TIMOTHY J.; Longobardi, Lara; Spagnoli, Anna; Miga, Michael I.

    2012-01-01

    An important concern in the study of fracture healing is the ability to assess mechanical integrity in response to candidate therapeutics in small-animal systems. In recent reports, it has been proposed that microCT image-derived densitometric parameters could be used as a surrogate for mechanical property assessment. Recently, we have proposed an inverse methodology that iteratively reconstructs the modulus of elasticity of the lumped soft callus/hard callus region by integrating both intrin...

  8. Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor

    DEFF Research Database (Denmark)

    Weber, Christian; Pohl, Sebastian; Pörtner, Ralf;

    2007-01-01

    The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive...... oxygen sensor for the continuous monitoring of the cultivation process. Fixed bed bioreactors offer advantages in comparison to other systems due to their ease of automation and online monitoring capability during the cultivation process. These benefits combined with the advantage of single-use make...... the fixed bed reactor an interesting option for GMP processes. The cultivation of the encapsulated cells in the fixed bed bioreactor system offered vitalities and adipogenic differentiation similar to well-mixed suspension cultures....

  9. Trend road safety measures : international course on transportation and road engineering in developing countries. Two-year postgraduate Diploma and M.Sc. programme.

    NARCIS (Netherlands)

    1991-01-01

    This course focuses mainly on traffic and transport in developing countries, and deals primarily with matters of infrastructure. Road safety and road safety problems are closely related to the construction and operation of the road network. (See also C 1341 - C1346).

  10. Differential effects of culture senescence and mechanical stimulation on the proliferation and leiomyogenic differentiation of MSC from different sources: implications for engineering vascular grafts.

    Science.gov (United States)

    Koobatian, Maxwell T; Liang, Mao-Shih; Swartz, Daniel D; Andreadis, Stelios T

    2015-04-01

    We examined the effects of senescence on the proliferation and leiomyogenic differentiation potential of mesenchymal stem cells (MSCs) isolated from bone marrow (BM-MSCs) or hair follicles (HF-MSCs). To this end, we compared ovine HF-MSCs and BM-MSCs in terms of their proliferation and differentiation potential to the smooth muscle cell lineage. We discovered that HF-MSCs are less susceptible to culture senescence compared with BM-MSCs. We hypothesized that application of mechanical forces may enhance the contractility and mechanical properties of vascular constructs prepared from senescent MSCs. Interestingly, HF-MSCs and BM-MSCs responded differently to changes in the mechanical microenvironment, suggesting that despite phenotypic similarities, MSCs from different anatomic locations may activate different pathways in response to the same microenvironmental factors. In turn, this may also suggest that cell-based tissue regeneration approaches may need to be tailored to the stem cell origin, donor age, and culture time for optimal results.

  11. "Design for All in the Context of the Information Society": Integration of a Specialist Course in a Generalist M.Sc. Program in Electrical and Electronics Engineering

    Science.gov (United States)

    Godino-Llorente, J. I.; Fraile, R.; Gonzalez de Sande, J. C.; Osma-Ruiz, V.; Saenz-Lechon, N.

    2012-01-01

    This paper describes an educational research experience that took place in the Electrical & Electronics Engineering Master's program offered at the Escuela Universitaria de Ingenieria Tecnica de Telecomunicacion, Universidad Politecnica de Madrid, Madrid, Spain. The focus is to provide details of the motivation behind and the design and subsequent…

  12. Continuous bottom temperature measurements in strategic areas of the Florida Reef Tract at MSC Diego Restoration Site, 2004 - 2006 (NODC Accession 0014320)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This ongoing project began in 1988. A total of 38 subsurface recording thermographs have been deployed in the Florida Keys National Marine Sanctuary (FKNMS)and at...

  13. Mesoporous calcium–silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2

    Directory of Open Access Journals (Sweden)

    Song W

    2015-03-01

    Full Text Available Wenhua Song,1,* Xiangde Li,1,* Jun Qian,1 Guoyu Lv,2 Yonggang Yan,2 Jiacan Su,3 Jie Wei1 1Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People’s Republic of China; 2College of Physical Science and Technology, Sichuan University, Chengdu, People’s Republic of China; 3Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China *These authors contributed equally to this paper Abstract: Mesoporous calcium–silicon xerogels with a pore size of 15 nm (MCS-15 and pore volume of 1.43 cm3/g were synthesized by using 1,3,5-mesitylene (TMB as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium–silicon xerogels with a pore size of 4 nm (MCS-4. The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2. The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g. Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release. The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction. Keywords: mesoporous calcium–silicon xerogels, pore size, pore volume, load-release, rhBMP-2

  14. 'You're depressed'; 'no I'm not': GPs' and patients' different models of depression. UMDS MSc in General Practice Teaching Group.

    OpenAIRE

    1999-01-01

    Questionnaires concerning models of depression were completed by 90 general practitioners (GPs) and 681 patients. GPs and patients held similar beliefs about the role of mood-related symptoms, psychological causes, and non-medical treatments. However, the GPs reported greater support for somatic symptoms, medical causes, and medical treatments. Therefore, GPs and patients report different models of depression, with GPs favouring a more medical perspective. In addition, the results illustrated...

  15. Experimental Study on Self-assembly of KLD-12 Peptide Hydrogel and 3-D Culture of MSC Encapsulated within Hydrogel In Vitro

    Institute of Scientific and Technical Information of China (English)

    Jianhua SUN; Qixin ZHENG

    2009-01-01

    o-fiber hydrogel in vitro. MSCs in KLD-12 peptide hydrogel grew well and proliferated with the culture time. KLD-12 peptide hydrogel can serve as an excellent injectable material of biological scaffolds in tissue engineering of IVD.

  16. Education and the Child Labor Paradox Today. Essay Review of "Children on the Streets of the Americas" (Roslyn A. Mickelson, editor); "The Policy Analysis of Child Labor: A Comparative Study" (Christiaan Grootaert, Harry Anthony Patrinos); "What Works for Working Children?" (Jo Boyden, Birgitta Ling, William Myers); "Child Employment in Britain: A Social and Psychological Analysis" (Sandy Hobbs, Jim McKechnie); and "Bud, Not Buddy" (Christopher Paul Curtis).

    Science.gov (United States)

    Post, David

    2001-01-01

    Reviews five books on child labor, published 1997-2000, with reference to the International Labour Organization's 1999 convention that retreats from its previous hard stance on child labor. Discusses street children; public policy on child labor, child welfare, and school attendance; types of children's work; and working children as agents…

  17. Sojuz muzõki i notshnõh sfer / Tamara Unanova

    Index Scriptorium Estoniae

    Unanova, Tamara

    2007-01-01

    Tallinnas Pirita kloostri varemetes Birgitta festivalil nähtust : Anton Rubinshteini ooper "Deemon" Moskva Novaja Opera esituses 16. aug., Arthur Honeggeri oratoorium "Jeanne d'Arc tuleriidal" 10. aug., Carl Orffi lavaline kantaat "Carmina Burana" 15. aug., lavastatud galakontsert filmikatketega ooperidiiva Maria Callasest 17. aug

  18. John Stuart Mill e il liberalismo italiano nell'età del positivismo

    OpenAIRE

    URBINATI, Nadia

    1989-01-01

    Defence date: 29 June 1989 Examining board: Athanasios Moulakis (I.U.E.), supervisor ; Norberto Bobbio (Università di Torino) ; Eugenio Garin (Scuola Normale Superiore di Pisa) ; Birgitta Nedelmann (I.U.E.), co-supervisor ; Antonio Santucci (Università di Bologna) First made available online: 23 September 2015

  19. Maailmaklassi Donizetti ja Britten / Harry Liivrand

    Index Scriptorium Estoniae

    Liivrand, Harry, 1961-

    2008-01-01

    Tallinnas 8.-17. augustini väldanud Birgitta festivalil esitatud ooperitest: Gaetano Donizetti "Maria Stuart" Moskva Novaja Opera esituses (esietendus 11. aug.), Benjamin Britteni "Kruvi keere" (esietendus 15. aug.), Pietro Mascagni "Talupoja au" ja Ruggiero Leoncavallo"Pajatsid" 10. augustil

  20. Pirita klooster tänasest ooperikires / Kersti Inno

    Index Scriptorium Estoniae

    Inno, Kersti, 1954-

    2006-01-01

    Birgitta Festivalist 11.-22. aug. Tallinnas Pirita kloostris. Astor Piazzolla tango-ooperist "Maria de Buenos Aires" (etendused 11. ja 12. aug. Pirita kloostri varemetes). Moskva Helikon Opera etendustest: 15. aug. Mozarti ooper "Tituse halastus" ja 17. aug. Shostakovitshi ooper "Mtsenski maakonna Lady Macbeth". Carl Orffi "Carmina Burana" 18. aug., Verdi "Reekviem" 19. aug. ja kontsert "Tuulatud Thule" 20. augustil

  1. Dramatic music brings the opera to Estonia / Joel Alas

    Index Scriptorium Estoniae

    Alas, Joel

    2006-01-01

    Etendustest Birgitta Festivali raames 11.-22. aug. Tallinnas Pirita kloostris: Astor Piazzolla tango-ooperist "Maria de Buenos Aires" 11. aug., W. A. Mozarti ooper "Tituse halastus" Moskva Helikon Opera esituses 15. aug., Shostakovitshi ooper "Mtsenski maakonna Lady Macbeth" 17. augustil

  2. Enne kui Rakvere teatrisse saabub vananaistesuvi / Pille-Riin Purje

    Index Scriptorium Estoniae

    Purje, Pille-Riin, 1963-

    2007-01-01

    Rakvere teatri suvest, valmistumisest suurejoonelise "Cyrano de Bergerac'i" väljatoomiseks septembris. Imaveres etendati William Shakespeare'i "MacBethi", Tallinnas Pirita kloostris avas 10. ja 11. augustil Birgitta Festivali Arthur Honeggeri-Paul Claudeli dramaatiline oratoorium "Jeanne d'Arc tuleriidal". Mõlemad lavastas Üllar Saaremäe

  3. Aasta betoonehitis 2001 / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2002-01-01

    Aasta betoonehitis: kuivpuistainete terminal Muuga sadamas (projekteerija Randväli & Karema). Eripreemiad: arhitetuuri eest - Püha Birgitta Ordu klooster Pirital (arhitektid Ra Luhse, Tanel Tuhal), inseneritöö eest - konteinerterminali kai Tallinna sadamas (projekteerija MERIN) ning Estconde büroohoone Tallinnas (arhitektid Jüri Okas, Marika Lõoke)

  4. Gorodu i ljudjam / Hannes Tamme

    Index Scriptorium Estoniae

    Tamme, Hannes, 1957-

    2002-01-01

    Aasta Betoonehitise nimetuse võitis Muuga kuivpuisteainete terminal, projekteerija Randväli&Karema AS (arh. Eva Mölder). Eriauhind arhitektuurse osa eest - Birgitta klooster Pirital Luhse, Tuhal). Eriauhind tellijale - büroohoone Tallinnas Toompuiestee 33 (Jüri Okas)

  5. The Medical School Retention Game

    DEFF Research Database (Denmark)

    O'Neill, Lotte Dyhrberg

    2011-01-01

    scores and dropout. REFERENCES 1. O’Neill L, Wallstedt B, Eika B, Hartvigsen J. Factors associated with dropout in medical education: a literature review. Med Educ (In press). 2. Urlings-Strop LC, Stijnen T, Themmen APN, Splinter TAW. Selection of medical students: a controlled experiment. Med Educ 2009......INTRODUCTION Very few studies have reported on the effect of admission tests on medical school dropout.1 Recently Urlings-Strop et al. found the relative risk of dropout to be 2.6 times lower for ‘selected students’ than for ‘lottery admitted controls’.2 The main aim of our study was to evaluate...... the predictive validity of admission testing versus grade-based admission on dropout. METHOD This prospective cohort study followed 6 cohorts of medical students admitted to the medical school at University of Southern Denmark (USD) in 2002-2007 (N=1544). Half the students were admitted based on highest prior...

  6. Evaluation of User Interface of DL + Federated Search Engine from the Perspective of M.A / M.S.C Students in Al-Zahra University in order to Provide Federated Search Engines User Interface Pattern

    Directory of Open Access Journals (Sweden)

    Amir Ghaebi

    2014-02-01

    The results showed that the major component from graduate students' perspective is guidance and navigation and searched filter components. Results indicate that more than 50 percent of students the importance of 74 criteria of 96 criteria considered high and very high and, (therefore the majority of the criteria in the study from the viewpoints are acceptable. However, findings also showed that among the four components of this study, the search filter, display and record review, consolidation and guidance is a positive relation, and between the views of different faculties is not significant difference about the importance of criterias and components.

  7. Evaluation of User Interface of DL + Federated Search Engine from the Perspective of M.A / M.S.C Students in Al-Zahra University in order to Provide Federated Search Engines User Interface Pattern

    OpenAIRE

    Amir Ghaebi; Roya Baradar; Mina Farnaghi

    2014-01-01

    The aim of the study is investigate the Federated search engine user interface criteria from perspective of Al-Zahra post graduate students. In this research, survey analysis has been used. The study populations were all post graduate students of Al-Zahra University. Because of the extensive community, stratified random and relative sampling was used and total 330 students were accepted as Sample that only 303 people participated in this study. For data collection a researcher made questionna...

  8. Simulasi Komputer Distribusi Tegangan Pada Helm Sepeda Motor Dari Bahan Komposit GFRP BTQN 157 EX Dilapisi Busa (Foam) Terhadap Beban Impak Kecepatan Tinggi Menggunakan MSC/NASTRAN 4.5

    OpenAIRE

    S.P, Parlindungan

    2011-01-01

    This study focuses on a verification using simulation of stress distribution of helmet motorcycle with high speed impact. The helmet is made of Glass Fiber Reinforced Plastics (GFRP). Two types of helmet model were used in this study, they are of composite helmet and foam-coated composite helmet. The helmets with load of 8.2MPa were impacted from several impact locations such as from the top, the side and the back of the helmets. To observe the stress distribution on the helmets, computer sim...

  9. Examining the Significance of Social Class in the Effectiveness of Celebrity Endorsed Cosmetic Advertisements by Emily Forster -2009- A Dissertation presented in part consideration for the degree of MSc International Business.

    OpenAIRE

    Forster, Emily

    2009-01-01

    Celebrity endorsements have become the most used and prevailing marketing strategy of advertising today in the UK. The ever-increasing public interest and fascination with celebrities provides marketers with the vast potential to promote their brands and expand their markets by capitalising on this phenomenon. Social class is conceptually complicated and methodologically challenging and yet continues to provide provoking insights into consumption choices. This study examines the impact ...

  10. Rationale and design of the first randomized, double-blind, placebo-controlled trial of intramyocardial injection of autologous bone-marrow derived Mesenchymal Stromal Cells in chronic ischemic Heart Failure (MSC-HF Trial)

    DEFF Research Database (Denmark)

    Mathiasen, Anders Bruun; Jørgensen, Erik; Qayyum, Abbas Ali;

    2012-01-01

    Stem cell therapy is an emerging treatment modality in cardiovascular disease. The best cell type and delivery method in different cardiovascular diseases remain to be determined.......Stem cell therapy is an emerging treatment modality in cardiovascular disease. The best cell type and delivery method in different cardiovascular diseases remain to be determined....

  11. C. Petrone et al.: "Magnetic measurement of the model magnet QD0 designed for the CLIC final focus beam transport line." CERN TE-MSC Internal Note, EDMS Nr: 1184196

    CERN Document Server

    Arpaia, Pasquale; Petrone, Carlo; Russenschuck, Stephan; Walckiers, Louis

    2012-01-01

    This note presents the results of the magnetic measurements performed on QD0, model magnet for the final focus transport line for CLIC (Fig. 1). This high-gradient, hybrid quadrupole has a yoke length of 0.1 m and an aperture of 8.3 mm. ND2Fe14B Permanent magnet blocks provide a gradient of 150 T/m, which can be further increased to 530 T/m when the four coils are excited to 18.3 A. The request was to measure the strength of the field and the multipole coefficients at different currents. The measurement of the field strength, by means of the single stretched wire system, was done in December 2011 in the I8 laboratory. The measurement of the multipole was done by means of the oscillating wire system [1][2].

  12. The first systematic analysis of 3D rapid prototyped poly(ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability

    International Nuclear Information System (INIS)

    Novel additive manufacturing processes are increasingly recognized as ideal techniques to produce 3D biodegradable structures with optimal pore size and spatial distribution, providing an adequate mechanical support for tissue regeneration while shaping in-growing tissues. With regard to the mechanical and biological performances of 3D scaffolds, pore size and geometry play a crucial role. In this study, a novel integrated automated system for the production and in vitro culture of 3D constructs, known as BioCell Printing, was used only to manufacture poly(ε-caprolactone) scaffolds for tissue engineering; the influence of pore size and shape on their mechanical and biological performances was investigated. Imposing a single lay-down pattern of 0°/90° and varying the filament distance, it was possible to produce scaffolds with square interconnected pores with channel sizes falling in the range of 245–433 µm, porosity 49–57% and a constant road width. Three different lay-down patterns were also adopted (0°/90°, 0°/60/120° and 0°/45°/90°/135°), thus resulting in scaffolds with quadrangular, triangular and complex internal geometries, respectively. Mechanical compression tests revealed a decrease of scaffold stiffness with the increasing porosity and number of deposition angles (from 0°/90° to 0°/45°/90°/135°). Results from biological analysis, carried out using human mesenchymal stem cells, suggest a strong influence of pore size and geometry on cell viability. On the other hand, after 21 days of in vitro static culture, it was not possible to detect any significant variation in terms of cell morphology promoted by scaffold topology. As a first systematic analysis, the obtained results clearly demonstrate the potential of the BioCell Printing process to produce 3D scaffolds with reproducible well organized architectures and tailored mechanical properties. (paper)

  13. Characteristics measured by the Eating Disorder Inventory for children at risk and protective factors for disordered eating in adolescent girls

    OpenAIRE

    Sanna Aila Gustafsson; Birgitta Edlund; Lars Kjellin; et al

    2010-01-01

    Sanna Aila Gustafsson1, Birgitta Edlund2, Lars Kjellin3, Claes Norring41Psychiatric Research Centre, School of Health and Medical Sciences, University of Örebro; 2Department of Public Health and Caring Sciences, University of Uppsala; 3Psychiatric Research Centre, University of Örebro; 4Centre for Psychiatry Research, Karolinska Institutet, Stockholm, SwedenObjective: The aim of this study was to examine longitudinally the role of characteristics measured by the Eating Disor...

  14. Racisme og identitet

    OpenAIRE

    Abelsen, Lena Kista; Jensen, Simone Meisner; Stadelund, Nynne Kjøller; Szøts, Anemone Thomas

    2014-01-01

    This study revolves around the subject of racism and identity. It investigates the change and development of racism and how identity has been perceived throughout the course of history. This investigation finds the combination of identity and racism important because of its belief in social constructivism. The primary theorists are Birgitta Frello and Stuart Hall. Edward Said also plays a significant role in understanding the Post-Colonial Era. The method in the study is inspired by Foucault’...

  15. "Deemon" ja "Jeanne d'Arc" / Harry Liivrand

    Index Scriptorium Estoniae

    Liivrand, Harry, 1961-

    2007-01-01

    Anton Rubinshteini ooperist "Deemon" Moskva Novaja Opera esituses 16. aug., Arthur Honeggeri oratooriumist "Jeanne d'Arc tuleriidal" 10. aug., Piazolla tango-ooperist "Maria de Buenos Aires" 14. aug., Carl Orffi lavalisest kantaadist "Carmina Burana" 15. aug., lavastatud galakontserdist filmikatketega ooperidiiva Maria Callasest 17. aug. ja klassikalise ja dzhässballeti õhtutest Vene Keiserlikult balletitrupilt 18. ja 19. aug. Birgitta festivali raames Tallinnas Pirita kloostri varemetes

  16. Comprehensive transcriptomic and proteomic characterization of human mesenchymal stem cells reveals source specific cellular markers.

    Science.gov (United States)

    Billing, Anja M; Ben Hamidane, Hisham; Dib, Shaima S; Cotton, Richard J; Bhagwat, Aditya M; Kumar, Pankaj; Hayat, Shahina; Yousri, Noha A; Goswami, Neha; Suhre, Karsten; Rafii, Arash; Graumann, Johannes

    2016-01-01

    Mesenchymal stem cells (MSC) are multipotent cells with great potential in therapy, reflected by more than 500 MSC-based clinical trials registered with the NIH. MSC are derived from multiple tissues but require invasive harvesting and imply donor-to-donor variability. Embryonic stem cell-derived MSC (ESC-MSC) may provide an alternative, but how similar they are to ex vivo MSC is unknown. Here we performed an in depth characterization of human ESC-MSC, comparing them to human bone marrow-derived MSC (BM-MSC) as well as human embryonic stem cells (hESC) by transcriptomics (RNA-seq) and quantitative proteomics (nanoLC-MS/MS using SILAC). Data integration highlighted and validated a central role of vesicle-mediated transport and exosomes in MSC biology and also demonstrated, through enrichment analysis, their versatility and broad application potential. Particular emphasis was placed on comparing profiles between ESC-MSC and BM-MSC and assessing their equivalency. Data presented here shows that differences between ESC-MSC and BM-MSC are similar in magnitude to those reported for MSC of different origin and the former may thus represent an alternative source for therapeutic applications. Finally, we report an unprecedented coverage of MSC CD markers, as well as membrane associated proteins which may benefit immunofluorescence-based applications and contribute to a refined molecular description of MSC. PMID:26857143

  17. Musculoskeletal Complaints Among 11-Year-Old Children and Associated Factors

    NARCIS (Netherlands)

    Hulsegge, Gerben; van Oostrom, Sandra H.; Picavet, H. Susan J.; Twisk, Jos W. R.; Postma, Dirkje S.; Kerkhof, Marjan; Smit, Henriette A.; Wijga, Alet H.

    2011-01-01

    Musculoskeletal complaints (MSC) are common among children, often persist into adolescence, and increase the risk of MSC in adulthood. Knowledge regarding determinants of MSC among children is limited. The aim of this study was to determine the prevalence of MSC at age 11 years and to examine associ

  18. Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood

    DEFF Research Database (Denmark)

    Berg, Lise Charlotte; Koch, Thomas Gadegaard; Heerkens, T.;

    2009-01-01

    including bone marrow and umbilical cord blood. The objective of this study was to provide an in vitro comparison of the chondrogenic potential in MSC derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC). Results: MSC from both sources produced tissue with cartilage-like morphology...

  19. A42

    OpenAIRE

    V. Yuzhakov; L. Sevankaeva; M. Konoplyannikov; N. Fomina; I. Ingel; Konoplyannikov, A.

    2015-01-01

    The mechanisms of mesenchymal stem cells (MSC) action on malignant neoplasms attract much attention nowadays. Studies relating to the MSC effect on tumors are contradictory and indicate that MSC has both stimulating and suppressive actions on experimental neoplasms. We have shown previously that a single MSC administration to tumor-bearing animals has an oncomodulating effect on the growth of tumors. Short-term stimulation of the growth of tumor nodes initially after the MSC injection was usu...

  20. Post-thaw non-cultured and post-thaw cultured equine cord blood mesenchymal stromal cells equally suppress lymphocyte proliferation in vitro.

    Directory of Open Access Journals (Sweden)

    Lynn B Williams

    Full Text Available Multipotent mesenchymal stromal cells (MSC are receiving increased attention for their non-progenitor immunomodulatory potential. Cryopreservation is commonly used for long-term storage of MSC. Post-thaw MSC proliferation is associated with a lag-phase in vitro. How this lag-phase affect MSC immunomodulatory properties is unknown. We hypothesized that in vitro there is no difference in lymphocyte suppression potential between quick-thawed cryopreserved equine cord blood (CB MSC immediately included in mixed lymphocyte reaction (MLR and same MSC allowed post-thaw culture time prior to inclusion in MLR. Cryopreserved CB-MSC from five unrelated foals were compared using two-way MLR. For each of the five unrelated MSC cultures, paired MLR assays of MSC allowed five days of post-thaw culture and MSC included in MLR assay immediately post-thawing were evaluated. We report no difference in the suppression of lymphocyte proliferation by CB-MSC that had undergone post-thaw culture and MSC not cultured post-thaw (p<0.0001. Also, there was no inter-donor variability between the lymphocyte suppressive properties of MSC harvested from the five different donors (p = 0.13. These findings suggest that cryopreserved CB-MSC may have clinical utility immediately upon thawing. One implication hereof is the possibility of using cryopreserved CB-MSC at third party locations without the need for cell culture equipment or competencies.

  1. Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

    Science.gov (United States)

    Lee, Ji Yong; Kim, Eiru; Choi, Seong-Mi; Kim, Dong-Wook; Kim, Kwang Pyo; Lee, Insuk; Kim, Han-Soo

    2016-01-01

    Transplantation of mesenchymal stem cells (MSCs) was reported to improve functional outcomes in a rat model of ischemic stroke, and subsequent studies suggest that MSC-derived microvesicles (MVs) can replace the beneficial effects of MSCs. Here, we evaluated three different MSC-derived MVs, including MVs from untreated MSCs (MSC-MVs), MVs from MSCs treated with normal rat brain extract (NBE-MSC-MVs), and MVs from MSCs treated with stroke-injured rat brain extract (SBE-MSC-MVs), and tested their effects on ischemic brain injury induced by permanent middle cerebral artery occlusion (pMCAO) in rats. NBE-MSC-MVs and SBE-MSC-MVs had significantly greater efficacy than MSC-MVs for ameliorating ischemic brain injury with improved functional recovery. We found similar profiles of key signalling proteins in NBE-MSC-MVs and SBE-MSC-MVs, which account for their similar therapeutic efficacies. Immunohistochemical analyses suggest that brain-extract—treated MSC-MVs reduce inflammation, enhance angiogenesis, and increase endogenous neurogenesis in the rat brain. We performed mass spectrometry proteomic analyses and found that the total proteomes of brain-extract—treated MSC-MVs are highly enriched for known vesicular proteins. Notably, MSC-MV proteins upregulated by brain extracts tend to be modular for tissue repair pathways. We suggest that MSC-MV proteins stimulated by the brain microenvironment are paracrine effectors that enhance MSC therapy for stroke injury. PMID:27609711

  2. En plats - En identitet

    OpenAIRE

    Gleisner, Jenny

    2006-01-01

    We live in a society where we are not bound to one place. We are encouraged to be mobile and constantly moving between different locations. The life in a convent is a contrast to this; nuns live at a specific place they are bounded to by vows and can not leave. The sisters in the convent of St. Birgitta, Pax Mariae have promised to stay at the same place and live by the rules of the convent. They live within the walls of the convent, an area with clear boarders. In this essay I analyse the me...

  3. Euroopas haruldane kloostrifestival / Heili Vaus-Tamm

    Index Scriptorium Estoniae

    Vaus-Tamm, Heili, 1961-

    2007-01-01

    Birgitta festivalil Pirita kloostri varemetes mängukavas: Arthur Honeggeri oratoorium "Jeanne d'Arc tuleriidal" (10. ja 11. aug., lavastajaks Üllar Saaremäe, peaosas Mirtel Pohla ja Benedictuse osatäitjaks Rain Simmul), Piazolla tango-ooper "Maria de Buenos Aires" (14. aug.), Carl Orffi lavaline kantaat "Carmina Burana" (15. aug.), A. Rubinshteini ooper "Deemon" (16. aug.), lavastatud galakontsert filmikatketega ooperidiiva Maria Callasest (17. aug.), klassikalise ja dzhässballeti õhtud Vene Keiserlikult balletitrupilt (18. ja 19. aug.)

  4. Morrissey

    Index Scriptorium Estoniae

    2009-01-01

    Laulja Morrissey kontserdist tuuri "Tour of Refusal" raames 4. juulil Tallinnas Rock Cafés, "IV Tallinna Kitarrifestivalist" 15.-20. juunil Mustpeade Majas, "Birgitta festivalist" 13.-23. augustil Pirita kloostris, rockmuusikafestivalist "Lelle Alternatiiv" 10.-12. juulil, "Tabasalu Jazz Festist" Rannamõisa kirikus ja Tabasalu keskväljakul 26.-27. juunil, muusikafestivalist "Klaaspärlimäng" Tartus ja Vormsil 16.-26. juulil, "VIII Eesti Noorte Heliloojate Festivalist" Tartu Jaani kirikus 4.-7. juunil, muusikafestivalist "Kumu ÖÖ" KUMUs 5. juunil, festivalist "Viru Folk" Käsmus 7.-9. augustil, "XVII Viljandi Pärimusmuusika Festivalist" 23.-26. juulil

  5. Suvefestivalid

    Index Scriptorium Estoniae

    2006-01-01

    2006. aasta suvel toimuvatest muusikaüritustest: Heliloojatele Kappidele pühendatud IX Suure-Jaani Muusikafestival, Nissi suvemuusika, Seitsme Linna muusika, Klaaspärlimäng, Pärnu ooperipäevad 2006, Tallinna XX orelifestival, Mravinski Festival 2006, Andrew Lloyd Webberi galaõhtu, Juu Jääb 2006 (retromuusikafestival), David Oistrahhi festival 2006, Hiiumaa VIII kammermuusikapäevad 2006, Viljandi vanamuusikafestival 2006, Rapla kirikumuusika XIV festival 2006, Haapsalu XIII keelpillimuusika festival, Kuressaare ooperipäevad 2006, Birgitta festival 2006, Võru Vaskpillipäevad 2006, Tallinna Kammermuusika festival 2006

  6. Decree no. 2001-1199 of the 10 december 2001 publishing the resolution MSC. 88 (71) notifying adoption of the international compilation of safety rules for the spent nuclear fuels, plutonium and high level radioactive wastes transport in casks on ships (compilation INF) (annexes), adopted at London the 27 may 1999; Decret no. 2001-1199 du 10 decembre 2001 portant publication de la resolution MSC.88 (71) portant adoption du recueil international de regles de securite pour le transport de combustible nucleaire irradie, de plutonium et de dechets hautement radioactifs en colis a bord de navires (recueil INF) (ensemble une annexe), adoptee a Londres le 27 mai 1999

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This legislative text concerns the safety rules of spent nuclear fuels, plutonium and high level radioactive wastes transport, in casks on ships. Rules, fire prevention, temperature control of casks, electric supply, radioprotection, management and emergency plans are detailed. (A.L.B.)

  7. Effects of high glucose on mesenchymal stem cell proliferation and differentiation

    International Nuclear Information System (INIS)

    High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized MSC (hMSC-TERT) and primary MSC (hMSC). HG (25 mM) enhanced hMSC-TERT proliferation in long-term studies in contrast to hMSC where proliferation was unchanged. Thioredoxin-interacting protein, which is involved in apoptosis regulation, was stimulated by glucose in hMSC-TERT. However, apoptosis was not influenced by HG in both cell types. MSC treatment with HG favored osteogenic differentiation. MSC are resistant to HG toxicity, depending on the stemness of MSC. Proliferation and osteogenic differentiation are stimulated by HG. Effects of HG on the transient amplifying compartment of MSC may differ from those in mature cells. Further research is needed to unravel the molecular mechanisms of HG resistance of MSC

  8. Mesenchymal stem cells are short-lived and do not migrate beyond the lungs after intravenous infusion

    Directory of Open Access Journals (Sweden)

    Elke eEggenhofer

    2012-09-01

    Full Text Available Mesenchymal stem cells (MSC are under investigation as a therapy for a variety of disorders. Although animal models show long term regenerative and immunomodulatory effects of MSC, the fate of MSC after infusion remains to be elucidated. In the present study the localization and viability of MSC was examined by isolation and re-culture of intravenously infused MSC. C57BL/6 MSC (500,000 constitutively expressing DsRed-fluorescent protein and radioactively labeled with Cr-51 were infused via the tail vein in wild type C57BL/6 mice. After 5min, 1h, 24h or 72h, mice were sacrificed and blood, lungs, liver, spleen, kidneys and bone marrow removed. One hour after MSC infusion the majority of Cr-51 was found in the lungs, whereas after 24h Cr-51 was mainly found in the liver. Tissue cultures demonstrated that viable donor MSC were present in the lungs up to 24h after infusion, after which they disappeared. No viable MSC were found in the other organs examined at any time. The induction of ischemia-reperfusion injury in the liver did not trigger the migration of viable MSC to the liver. These results demonstrate that MSC are short-lived after i.v. infusion and that viable MSC do not pass the lungs. Cell debris may be transported to the liver. Long term immunomodulatory and regenerative effects of infused MSC must therefore be mediated via other cell types.

  9. 人骨髓间质干细胞ING4基因的克隆及其慢病毒表达载体构建%Cloning of human ING4 drived from MSC and construction of PNL-ING4 Lentiviral vector

    Institute of Scientific and Technical Information of China (English)

    张蕾蕾; 许文荣; 乔纯; 钱晖; 朱伟; 李继刚; 周洪兴; 司煜安; 周宗海; 阴晴

    2007-01-01

    目的:克隆人骨髓间质干细胞ING4(inhibitor of growth famility,member4)基因,构建其慢病毒表达载体PNL-ING4.方法:提取人骨髓间质干细胞(hMSCs)总RNA,经RT-PCR扩增出ING4cDNA,克隆至PMD19-T载体,选择阳性克隆进行酶切鉴定和测序,构建慢病毒表达载体PNL-ING4,用双酶切、基因测序进行鉴定.结果:RT-PCR产物为750 bp的条带,双酶切和基因测序正确.结论:成功从hMSCs克隆了ING4基因并成功构建其慢病毒表达载体PNL-ING4,为进一步研究ING4基因的作用与抗肿瘤机制奠定了基础.

  10. Selvevaluering af elektro-civilingeniøruddannelsen ved DTU

    DEFF Research Database (Denmark)

    Bruun, Erik; Galster, Georg; Hansen, Ole;

    1997-01-01

    This report describes a self-evaluation of the electrical and electronics engineering M.Sc. education at DTU.......This report describes a self-evaluation of the electrical and electronics engineering M.Sc. education at DTU....

  11. Characterization of Human Mesenchymal Stem Cells from Ewing Sarcoma Patients. Pathogenetic Implications

    Science.gov (United States)

    Amaral, Ana Teresa; Manara, Maria Cristina; Berghuis, Dagmar; Ordóñez, José Luis; Biscuola, Michele; Lopez-García, Maria Angeles; Osuna, Daniel; Lucarelli, Enrico; Alviano, Francesco; Lankester, Arjan; Scotlandi, Katia; de Álava, Enrique

    2014-01-01

    Background Ewing Sarcoma (EWS) is a mesenchymal-derived tumor that generally arises in bone and soft tissue. Intensive research regarding the pathogenesis of EWS has been insufficient to pinpoint the early events of Ewing sarcomagenesis. However, the Mesenchymal Stem Cell (MSC) is currently accepted as the most probable cell of origin. Materials and Methods In an initial study regarding a deep characterization of MSC obtained specifically from EWS patients (MSC-P), we compared them with MSC derived from healthy donors (MSC-HD) and EWS cell lines. We evaluated the presence of the EWS-FLI1 gene fusion and EWSR1 gene rearrangements in MSC-P. The presence of the EWS transcript was confirmed by q-RT-PCR. In order to determine early events possibly involved in malignant transformation, we used a multiparameter quantitative strategy that included both MSC immunophenotypic negative/positive markers, and EWS intrinsic phenotypical features. Markers CD105, CD90, CD34 and CD45 were confirmed in EWS samples. Results We determined that MSC-P lack the most prevalent gene fusion, EWSR1-FLI1 as well as EWSR1 gene rearrangements. Our study also revealed that MSC-P are more alike to MSC-HD than to EWS cells. Nonetheless, we also observed that EWS cells had a few overlapping features with MSC. As a relevant example, also MSC showed CD99 expression, hallmark of EWS diagnosis. However, we observed that, in contrast to EWS cells, MSC were not sensitive to the inhibition of CD99. Conclusions In conclusion, our results suggest that MSC from EWS patients behave like MSC-HD and are phenotypically different from EWS cells, thus raising important questions regarding MSC role in sarcomagenesis. PMID:24498265

  12. Aspiration, but not injection, decreases cultured equine mesenchymal stromal cell viability

    OpenAIRE

    Williams, Lynn B.; Russell, Keith A.; Koenig, Judith B.; Thomas G. Koch

    2016-01-01

    Background Recently, equine multipotent mesenchymal stromal cells (MSC) have received significant attention as therapy for various conditions due to their proposed regenerative and immune-modulating capacity. MSC are commonly administered to the patient through a hypodermic needle. Currently, little information is available on the effect of such injection has on equine MSC immediate and delayed viability. We hypothesize that viability of equine MSC is not correlated with needle diameter durin...

  13. Stem cell therapy independent of stemness

    OpenAIRE

    Lee, Techung

    2012-01-01

    Mesenchymal stem cell (MSC) therapy is entering a new era shifting the focus from initial feasibility study to optimization of therapeutic efficacy. However, how MSC therapy facilitates tissue regeneration remains incompletely characterized. Consistent with the emerging notion that secretion of multiple growth factors/cytokines (trophic factors) by MSC provides the underlying tissue regenerative mechanism, the recent study by Bai et al demonstrated a critical therapeutic role of MSC-derived h...

  14. Human Allogeneic Bone Marrow and Adipose Tissue Derived Mesenchymal Stromal Cells Induce CD8+ Cytotoxic T Cell Reactivity

    OpenAIRE

    Roemeling-van Rhijn, Marieke; Reinders, Marlies E.; Franquesa, Marcella; Engela, Anja U; Korevaar, Sander S; Roelofs, Helene; Genever, Paul G; IJzermans, Jan NM; Betjes, Michiel GH; Baan, Carla C; Weimar, Willem; Hoogduijn, Martin J.

    2013-01-01

    Introduction For clinical applications, Mesenchymal Stromal Cells (MSC) can be isolated from bone marrow and adipose tissue of autologous or allogeneic origin. Allogeneic cell usage has advantages but may harbor the risk of sensitization against foreign HLA. Therefore, we evaluated whether bone marrow and adipose tissue-derived MSC are capable of inducing HLA-specific alloreactivity. Methods MSC were isolated from healthy human Bone Marrow (BM-MSC) and adipose tissue (ASC) donors. Peripheral ...

  15. Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repair.

    Science.gov (United States)

    Gnecchi, Massimiliano; Danieli, Patrizia; Malpasso, Giuseppe; Ciuffreda, Maria Chiara

    2016-01-01

    Tissue regeneration from transplanted mesenchymal stromal cells (MSC) either through transdifferentiation or cell fusion was originally proposed as the principal mechanism underlying their therapeutic action. However, several studies have now shown that both these mechanisms are very inefficient. The low MSC engraftment rate documented in injured areas also refutes the hypothesis that MSC repair tissue damage by replacing cell loss with newly differentiated cells. Indeed, despite evidence of preferential homing of MSC to the site of myocardial ischemia, exogenously administered MSC show poor survival and do not persist in the infarcted area. Therefore, it has been proposed that the functional benefits observed after MSC transplantation in experimental models of tissue injury might be related to the secretion of soluble factors acting in a paracrine fashion. This hypothesis is supported by pre-clinical studies demonstrating equal or even improved organ function upon infusion of MSC-derived conditioned medium (MSC-CM) compared with MSC transplantation. Identifying key MSC-secreted factors and their functional role seems a reasonable approach for a rational design of nextgeneration MSC-based therapeutics. Here, we summarize the major findings regarding both different MSC-mediated paracrine actions and the identification of paracrine mediators. PMID:27236669

  16. 48 CFR 247.573-1 - Ocean transportation incidental to a contract for supplies, services, or construction.

    Science.gov (United States)

    2010-10-01

    ... Business Management Directorate, MSC; or (ii) The Commander, Military Surface Deployment and Distribution (SDDC), through the SDDC global e-mailbox sddc.ops.ffw@us.army.mil and the Principal Assistant... Commander, MSC, through the Contracts and Business Management Directorate, MSC; or (B) The...

  17. Hypoxic culture conditions for Mesenchymal Stromal/Stem Cells from Wharton's jelly: a critical parameter to consider in a therapeutic context.

    Science.gov (United States)

    Reppel, Loic; Margossian, Talar; Yaghi, Layale; Moreau, Philippe; Mercier, Nathalie; Leger, Leonore; Hupont, Sebastien; Stoltz, Jean-Francois; Bensoussan, Daniele; Huselstein, Celine

    2014-01-01

    Mesenchymal Stromal/Stem Cells from human Wharton's jelly (WJ-MSC) are an abundant and interesting source of stem cells for applications in cell and tissue engineering. Their fetal origin confers specific characteristics compared to Mesenchymal Stromal/Stem Cells isolated from human bone marrow (BM-MSC). The aim of this work was to optimize WJ-MSC culture conditions for their subsequent clinical use. We focused on the influence of oxygen concentration during monolayer expansion on several parameters to characterize MSC. Our work distinguished WJ-MSC from BM-MSC in terms of proliferation, telomerase activity and adipogenic differentiation. We also showed that hypoxia had a beneficial effect on proliferation potential, clonogenic capacity and to a lesser extent, on HLA-G expression of WJ-MSC during their expansion. Moreover, we reported for the first time an increase in chondrogenic differentiation when WJ-MSC were expanded under hypoxia. In an allogeneic therapeutic context, production of clinical batches requires generating high numbers of MSC whilst maintaining the cells' properties. Considering our results, hypoxia will be an important parameter to take into account. In addition, the clinical use of WJ-MSC would provide significant numbers of cells with maintenance of their proliferation and differentiation potential, particularly their chondrogenic potential. Due to their chondrogenic differentiation potential, WJ-MSC promise to be an interesting source of MSC for cell therapy or tissue engineering for cartilage repair and/or regeneration.

  18. Parameters in three-dimensional osteospheroids of telomerized human mesenchymal (stromal) stem cells grown on osteoconductive scaffolds that predict in vivo bone-forming potential

    DEFF Research Database (Denmark)

    Burns, Jorge S; Hansen, Pernille Lund; Larsen, Kenneth H;

    2010-01-01

    Osteoblastic differentiation of human mesenchymal stem cells (hMSC) in monolayer culture is artefactual, lacking an organized bone-like matrix. We present a highly reproducible microwell protocol generating three-dimensional ex vivo multicellular aggregates of telomerized hMSC (hMSC-telomerase re...

  19. Mesenchymal stem cells are short-lived and do not migrate beyond the lungs after intravenous infusion

    NARCIS (Netherlands)

    E. Eggenhofer (Elke); V. Benseler (Volker); H.K. Kroemer (Heyo); F. Popp (Felix); E.K. Geissler (Edward); H.J. Schlitt (Hans); C.C. Baan (Carla); M.H. Dahlke (Marc); M.J. Hoogduijn (Martin)

    2012-01-01

    textabstractMesenchymal stem cells (MSC) are under investigation as a therapy for a variety of disorders. Although animal models show long term regenerative and immunomodulatory effects of MSC, the fate of MSC after infusion remains to be elucidated. In the present study the localization and viabili

  20. A modeling perspective on spatial and temporal variations in Messinian evaporite deposits

    NARCIS (Netherlands)

    Topper, R.P.M.; Meijer, P.Th.

    2013-01-01

    During the Messinian Salinity Crisis (MSC), evaporite-dominated sequences formed in marginal and deep basins of the Mediterranean Sea. In the marginal basins, the onset of the MSC is synchronous and a single depositional framework fits all sequences. In contrast, MSC sequences of the western and eas

  1. Current view of mesenchymal stem cells biology (brief review

    Directory of Open Access Journals (Sweden)

    Maslova O. A.

    2012-06-01

    Full Text Available Although mesenchymal stem cells (MSC are in a focus of attention, some aspects of their biology are still unclear. This paper is a review of current research on MSC biology. The use of MSC in regenerative medicine is also briefly discussed.

  2. Primary mesenchymal stem cells in human transplanted lungs are CD90/CD105 perivascularly located tissue-resident cells

    DEFF Research Database (Denmark)

    Rolandsson, Sara; Andersson Sjöland, Annika; Brune, Jan C;

    2014-01-01

    . This study therefore aimed to identify and characterise the 'bona fide' MSC in human lungs and to investigate if the MSC numbers correlate with the development of bronchiolitis obliterans syndrome in lung-transplanted patients. METHODS: Primary lung MSC were directly isolated or culture-derived from central...

  3. PDX1- and NGN3-mediated in vitro reprogramming of human bone marrow-derived mesenchymal stromal cells into pancreatic endocrine lineages

    DEFF Research Database (Denmark)

    Limbert, Catarina; Päth, Günter; Ebert, Regina;

    2011-01-01

    Reprogramming of multipotent adult bone marrow (BM)-derived mesenchymal stromal/stem cells (MSC) (BM-MSC) represents one of several strategies for cell-based therapy of diabetes. However, reprogramming primary BM-MSC into pancreatic endocrine lineages has not yet been consistently demonstrated....

  4. Cerebral transplantation of encapsulated mesenchymal stem cells improves cellular pathology after experimental traumatic brain injury

    DEFF Research Database (Denmark)

    Heile, Anna M B; Wallrapp, Christine; Klinge, Petra M;

    2009-01-01

    PURPOSE: "Naked" human mesenchymal stem cells (MSC) are neuro-protective in experimental brain injury (TBI). In a controlled cortical impact (CCI) rat model, we investigated whether encapsulated MSC (eMSC) act similarly, and whether efficacy is augmented using cells transfected to produce the neu...

  5. Impact of Squamous Differentiation in Breast Carcinoma.

    Science.gov (United States)

    Pai, Trupti; Shet, Tanuja; Desai, Sangeeta; Patil, Asawari; Nair, Nita; Parmar, Vani; Gupta, Sudeep; Budrukkar, Ashwini; Sarin, Rajiv; Badwe, Rajendra

    2016-09-01

    This study attempted to review the impact of extent of squamous differentiation in 56 infiltrating duct carcinomas (IDC) with squamous differentiation (metaplastic squamous carcinomas [MSC]). Tumors showing 100% squamous elements were labeled as primary squamous carcinomas (PSC; n = 28) and compared with 28 MSC showing lesser squamous components. A clinicopathological comparison revealed that lymphovascular emboli were never seen in any PSC but were noted in 7/28 of other MSC, while perineural invasion was seen only in PSC and not in MSC. Nodal metastasis was significantly more in other MSC as opposed to PSC. Most MSC presented with 2- to 5-cm sized tumors while PSC were 5 to 10 cm in size. PSC showed cystic change while MSC did not. Disease free survival (DFS) for MSC versus PSC was 64% versus 39.8%, while overall survival (OAS) was 72.7% in MSC versus 66.7% in PSC. Tumor stage affected DFS in MSC while none of the factors affected DFS/OAS in PSC. The extent of squamous differentiation affected DFS with best behavior for metaplastic carcinomas showing 90% squamous component (P = .024). PSC of breast is an aggressive disease and show distinct clinicopathological features from other MSC, and though the current definition of MSC does not advocate quantifying the squamous element, clearly this affects prognosis. PMID:27076454

  6. Impact of bacteria and bacterial components on osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Fiedler, Tomas, E-mail: tomas.fiedler@med.uni-rostock.de [Institute for Medical Microbiology, Virology, and Hygiene, Rostock University Medical Center, Schillingallee 70, D-18057 Rostock (Germany); Salamon, Achim; Adam, Stefanie; Herzmann, Nicole [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany); Taubenheim, Jan [Institute for Medical Microbiology, Virology, and Hygiene, Rostock University Medical Center, Schillingallee 70, D-18057 Rostock (Germany); Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany); Peters, Kirsten [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany)

    2013-11-01

    Adult mesenchymal stem cells (MSC) are present in several tissues, e.g. bone marrow, heart muscle, brain and subcutaneous adipose tissue. In invasive infections MSC get in contact with bacteria and bacterial components. Not much is known about how bacterial pathogens interact with MSC and how contact to bacteria influences MSC viability and differentiation potential. In this study we investigated the impact of three different wound infection relevant bacteria, Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes, and the cell wall components lipopolysaccharide (LPS; Gram-negative bacteria) and lipoteichoic acid (LTA; Gram-positive bacteria) on viability, proliferation, and osteogenic as well as adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (adMSC). We show that all three tested species were able to attach to and internalize into adMSC. The heat-inactivated Gram-negative E. coli as well as LPS were able to induce proliferation and osteogenic differentiation but reduce adipogenic differentiation of adMSC. Conspicuously, the heat-inactivated Gram-positive species showed the same effects on proliferation and adipogenic differentiation, while its cell wall component LTA exhibited no significant impact on adMSC. Therefore, our data demonstrate that osteogenic and adipogenic differentiation of adMSC is influenced in an oppositional fashion by bacterial antigens and that MSC-governed regeneration is not necessarily reduced under infectious conditions. - Highlights: • Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli bind to and internalize into adMSC. • Heat-inactivated cells of these bacterial species trigger proliferation of adMSC. • Heat-inactivated E. coli and LPS induce osteogenic differentiation of adMSC. • Heat-inactivated E. coli and LPS reduce adipogenic differentiation of adMSC. • LTA does not influence adipogenic or osteogenic differentiation of adMSC.

  7. Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium.

    Science.gov (United States)

    Danieli, Patrizia; Malpasso, Giuseppe; Ciuffreda, Maria Chiara; Gnecchi, Massimiliano

    2016-01-01

    Mesenchymal stem cells (MSC) produce and secrete a great variety of cytokines and chemokines that play beneficial paracrine actions when MSC are used for tissue repair. The conditioned medium (CM) derived from MSC can be used both in vitro and in vivo to test specific paracrine effects or to screen putative paracrine/autocrine mediators by proteomics.In this chapter, we describe a straightforward method to prepare MSC-derived CM. Furthermore, we summarize some in vitro assays useful for testing the cytoprotective, angiogenic, and regenerative activity of CM. These assays are very helpful when studying the role of MSC in cardiac repair and regeneration. PMID:27236688

  8. Signatures of protein structure in the cooperative gating of mechanosensitive ion channels

    CERN Document Server

    Kahraman, Osman; Haselwandter, Christoph A

    2016-01-01

    Membrane proteins deform the surrounding lipid bilayer, which can lead to membrane-mediated interactions between neighboring proteins. Using the mechanosensitive channel of large conductance (MscL) as a model system, we demonstrate how the observed differences in protein structure can affect membrane-mediated interactions and cooperativity among membrane proteins. We find that distinct oligomeric states of MscL lead to distinct gateway states for the clustering of MscL, and predict signatures of MscL structure and spatial organization in the cooperative gating of MscL. Our modeling approach establishes a quantitative relation between the observed shapes and cooperative function of membrane~proteins.

  9. Novel application of stem cell-derived factors for periodontal regeneration

    Energy Technology Data Exchange (ETDEWEB)

    Inukai, Takeharu, E-mail: t-inukai@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Katagiri, Wataru, E-mail: w-kat@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Yoshimi, Ryoko, E-mail: lianzi@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Osugi, Masashi, E-mail: masashi@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Kawai, Takamasa, E-mail: takamasa@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Hibi, Hideharu, E-mail: hibihi@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan); Ueda, Minoru, E-mail: mueda@med.nagoya-u.ac.jp [Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine (Japan)

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer Mesenchymal stem cells (MSCs) secrete a variety of cytokines. Black-Right-Pointing-Pointer Cytokines were detected in conditioned medium from cultured MSCs (MSC-CM). Black-Right-Pointing-Pointer MSC-CM enhanced activation of dog MSCs and periodontal ligament cells. Black-Right-Pointing-Pointer MSC-CM significantly promoted alveolar bone and cementum regeneration. Black-Right-Pointing-Pointer Multiple cytokines contained in MSC-CM promote periodontal regeneration. -- Abstract: The effect of conditioned medium from cultured mesenchymal stem cells (MSC-CM) on periodontal regeneration was evaluated. In vitro, MSC-CM stimulated migration and proliferation of dog MSCs (dMSCs) and dog periodontal ligament cells (dPDLCs). Cytokines such as insulin-like growth factor, vascular endothelial growth factor, transforming growth factor-{beta}1, and hepatocyte growth factor were detected in MSC-CM. In vivo, one-wall critical-size, intrabony periodontal defects were surgically created in the mandible of dogs. Dogs with these defects were divided into three groups that received MSC-CM, PBS, or no implants. Absorbable atelo-collagen sponges (TERUPLUG Registered-Sign ) were used as a scaffold material. Based on radiographic and histological observation 4 weeks after transplantation, the defect sites in the MSC-CM group displayed significantly greater alveolar bone and cementum regeneration than the other groups. These findings suggest that MSC-CM enhanced periodontal regeneration due to multiple cytokines contained in MSC-CM.

  10. Novel application of stem cell-derived factors for periodontal regeneration

    International Nuclear Information System (INIS)

    Highlights: ► Mesenchymal stem cells (MSCs) secrete a variety of cytokines. ► Cytokines were detected in conditioned medium from cultured MSCs (MSC-CM). ► MSC-CM enhanced activation of dog MSCs and periodontal ligament cells. ► MSC-CM significantly promoted alveolar bone and cementum regeneration. ► Multiple cytokines contained in MSC-CM promote periodontal regeneration. -- Abstract: The effect of conditioned medium from cultured mesenchymal stem cells (MSC-CM) on periodontal regeneration was evaluated. In vitro, MSC-CM stimulated migration and proliferation of dog MSCs (dMSCs) and dog periodontal ligament cells (dPDLCs). Cytokines such as insulin-like growth factor, vascular endothelial growth factor, transforming growth factor-β1, and hepatocyte growth factor were detected in MSC-CM. In vivo, one-wall critical-size, intrabony periodontal defects were surgically created in the mandible of dogs. Dogs with these defects were divided into three groups that received MSC-CM, PBS, or no implants. Absorbable atelo-collagen sponges (TERUPLUG®) were used as a scaffold material. Based on radiographic and histological observation 4 weeks after transplantation, the defect sites in the MSC-CM group displayed significantly greater alveolar bone and cementum regeneration than the other groups. These findings suggest that MSC-CM enhanced periodontal regeneration due to multiple cytokines contained in MSC-CM.

  11. Delivery of human mesenchymal adipose-derived stem cells restores multiple urological dysfunctions in a rat model mimicking radical prostatectomy damages through tissue-specific paracrine mechanisms.

    Science.gov (United States)

    Yiou, René; Mahrouf-Yorgov, Meriem; Trébeau, Céline; Zanaty, Marc; Lecointe, Cécile; Souktani, Richard; Zadigue, Patricia; Figeac, Florence; Rodriguez, Anne-Marie

    2016-02-01

    Urinary incontinence (UI) and erectile dysfunction (ED) are the most common functional urological disorders and the main sequels of radical prostatectomy (RP) for prostate cancer. Mesenchymal stem cell (MSC) therapy holds promise for repairing tissue damage due to RP. Because animal studies accurately replicating post-RP clinical UI and ED are lacking, little is known about the mechanisms underlying the urological benefits of MSC in this setting. To determine whether and by which mechanisms MSC can repair damages to both striated urethral sphincter (SUS) and penis in the same animal, we delivered human multipotent adipose stem cells, used as MSC model, in an immunocompetent rat model replicating post-RP UI and ED. In this model, we demonstrated by using noninvasive methods in the same animal from day 7 to day 90 post-RP injury that MSC administration into both the SUS and the penis significantly improved urinary continence and erectile function. The regenerative effects of MSC therapy were not due to transdifferentiation and robust engraftment at injection sites. Rather, our results suggest that MSC benefits in both target organs may involve a paracrine process with not only soluble factor release by the MSC but also activation of the recipient's secretome. These two effects of MSC varied across target tissues and damaged-cell types. In conclusion, our work provides new insights into the regenerative properties of MSC and supports the ability of MSC from a single source to repair multiple types of damage, such as those seen after RP, in the same individual.

  12. Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation.

    Science.gov (United States)

    Yang, Zijiang; Concannon, John; Ng, Kelvin S; Seyb, Kathleen; Mortensen, Luke J; Ranganath, Sudhir; Gu, Fangqi; Levy, Oren; Tong, Zhixiang; Martyn, Keir; Zhao, Weian; Lin, Charles P; Glicksman, Marcie A; Karp, Jeffrey M

    2016-07-26

    Pre-treatment or priming of mesenchymal stem cells (MSC) prior to transplantation can significantly augment the immunosuppressive effect of MSC-based therapies. In this study, we screened a library of 1402 FDA-approved bioactive compounds to prime MSC. We identified tetrandrine as a potential hit that activates the secretion of prostaglandin E2 (PGE2), a potent immunosuppressive agent, by MSC. Tetrandrine increased MSC PGE2 secretion through the NF-κB/COX-2 signaling pathway. When co-cultured with mouse macrophages (RAW264.7), tetrandrine-primed MSC attenuated the level of TNF-α secreted by RAW264.7. Furthermore, systemic transplantation of primed MSC into a mouse ear skin inflammation model significantly reduced the level of TNF-α in the inflamed ear, compared to unprimed cells. Screening of small molecules to pre-condition cells prior to transplantation represents a promising strategy to boost the therapeutic potential of cell therapy.

  13. Construction of metastatic spinal cancer tissue microarrays

    Institute of Scientific and Technical Information of China (English)

    Yang Xinghai; Chen Huajiang; Xiao Jianru; Yuan Wen; Jia Lianshun

    2009-01-01

    Objective: To explore the construction of metastatic spinal cancer (MSC) tissue microarrays and validate its value in immunohistochemical study of MSC. Methods: Paraffin-embedded specimens from 71 MSC cases and 6 primary tumor cases were selected as donor blocks and prepared into MSC tissue microarrays by tissue array arrangement, the steps of which included location, punching, sampling, sample seeding, and re-diagnosis by hematoxylin-eosin (HE) as well as MMP-9 and MMP-14 immunohistochemical staining. Results: The MSC tissue microarrays thus constructed were intact and crackless, containing 154 complete and well arranged microarray points. None of the sectioned tissue microarrays was lost, and the results of HE staining was consistent with the primary pathologic diagnoses. Immunohistochemical staining was also good without non-specific or marginal effect. Conclusion: The MSC tissue microarrays have a high value in the immunohistochemical study of MSC.

  14. Umbilical cord fibroblasts: Could they be considered as mesenchymal stem cells?

    Institute of Scientific and Technical Information of China (English)

    Mustapha; Zeddou; Biserka; Relic; Michel; G; Malaise

    2014-01-01

    In cell therapy protocols, many tissues were proposed as a source of mesenchymal stem cells(MSC) isolation. So far, bone marrow(BM) has been presented as the main source of MSC despite the invasive isolation pro-cedure related to this source. During the last years, the umbilical cord(UC) matrix was cited in different studies as a reliable source from which long term ex vivo prolif-erating fibroblasts were isolated but with contradictory data about their immunophenotype, gene expression profile, and differentiation potential. Hence, an inter-esting question emerged: Are cells isolated from cord matrix(UC-MSC) different from other MSCs? In this re-view, we will summarize different studies that isolated and characterized UC-MSC. Considering BM-MSC as gold standard, we will discuss if UC-MSC fulfill different criteria that define MSC, and what remain to be done in this issue.

  15. Proteomics reveals multiple routes to the osteogenic phenotype in mesenchymal stem cells

    Directory of Open Access Journals (Sweden)

    Yener Bülent

    2007-10-01

    Full Text Available Abstract Background Recently, we demonstrated that human mesenchymal stem cells (hMSC stimulated with dexamethazone undergo gene focusing during osteogenic differentiation (Stem Cells Dev 14(6: 1608–20, 2005. Here, we examine the protein expression profiles of three additional populations of hMSC stimulated to undergo osteogenic differentiation via either contact with pro-osteogenic extracellular matrix (ECM proteins (collagen I, vitronectin, or laminin-5 or osteogenic media supplements (OS media. Specifically, we annotate these four protein expression profiles, as well as profiles from naïve hMSC and differentiated human osteoblasts (hOST, with known gene ontologies and analyze them as a tensor with modes for the expressed proteins, gene ontologies, and stimulants. Results Direct component analysis in the gene ontology space identifies three components that account for 90% of the variance between hMSC, osteoblasts, and the four stimulated hMSC populations. The directed component maps the differentiation stages of the stimulated stem cell populations along the differentiation axis created by the difference in the expression profiles of hMSC and hOST. Surprisingly, hMSC treated with ECM proteins lie closer to osteoblasts than do hMSC treated with OS media. Additionally, the second component demonstrates that proteomic profiles of collagen I- and vitronectin-stimulated hMSC are distinct from those of OS-stimulated cells. A three-mode tensor analysis reveals additional focus proteins critical for characterizing the phenotypic variations between naïve hMSC, partially differentiated hMSC, and hOST. Conclusion The differences between the proteomic profiles of OS-stimulated hMSC and ECM-hMSC characterize different transitional phenotypes en route to becoming osteoblasts. This conclusion is arrived at via a three-mode tensor analysis validated using hMSC plated on laminin-5.

  16. Placenta Mesenchymal Stem Cell Derived Exosomes Confer Plasticity on Fibroblasts.

    Science.gov (United States)

    Tooi, Masayuki; Komaki, Motohiro; Morioka, Chikako; Honda, Izumi; Iwasaki, Kengo; Yokoyama, Naoki; Ayame, Hirohito; Izumi, Yuichi; Morita, Ikuo

    2016-07-01

    Mesenchymal stem cell (MSC)-conditioned medium (MSC-CM) has been reported to enhance wound healing. Exosomes contain nucleic acids, proteins, and lipids, and function as an intercellular communication vehicle for mediating some paracrine effects. However, the function of MSC-derived exosomes (MSC-exo) remains elusive. In this study, we isolated human placenta MSC (PlaMSC)-derived exosomes (PlaMSC-exo) and examined their function in vitro. PlaMSCs were isolated from human term placenta using enzymatic digestion. PlaMSC-exo were prepared from the conditioned medium of PlaMSC (PlaMSC-CM) by ultracentrifugation. The expression of stemness-related genes, such as OCT4 and NANOG, in normal adult human dermal fibroblasts (NHDF) after incubation with PlaMSC-exo was measured by real-time reverse transcriptase PCR analysis (real-time PCR). The effect of PlaMSC-exo on OCT4 transcription activity was assessed using Oct4-EGFP reporter mice-derived dermal fibroblasts. The stimulating effects of PlaMSC-exo on osteoblastic and adipocyte-differentiation of NHDF were evaluated by alkaline phosphatase (ALP), and Alizarin red S- and oil red O-staining, respectively. The expression of osteoblast- and adipocyte-related genes was also assessed by real-time PCR. The treatment of NHDF with PlaMSC-exo significantly upregulated OCT4 and NANOG mRNA expression. PlaMSC-exo also enhanced OCT4 transcription. The NHDF treated with PlaMSC-exo exhibited osteoblastic and adipocyte-differentiation in osteogenic and adipogenic induction media. PlaMSC-exo increase the expression of OCT4 and NANOG mRNA in fibroblasts. As a result, PlaMSC-exo influence the differentiation competence of fibroblasts to both osteoblastic and adipocyte-differentiation. It shows a new feature of MSCs and the possibility of clinical application of MSC-exo. J. Cell. Biochem. 117: 1658-1670, 2016. © 2015 Wiley Periodicals, Inc. PMID:26640165

  17. Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

    Directory of Open Access Journals (Sweden)

    Van Tendeloo Viggo FI

    2007-12-01

    Full Text Available Abstract Background Bone marrow-derived stromal cells (MSC are attractive targets for ex vivo cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (hMSC with enhanced green fluorescent protein (EGFP and neurotrophin (NT3. Three genetically modified hMSC lines (EGFP, NT3, NT3-EGFP were established and used to study cell survival and transgene expression following transplantation in rat spinal cord. Results First, we demonstrate long-term survival of transplanted hMSC-EGFP cells in rat spinal cord under, but not without, appropriate immune suppression. Next, we examined the stability of EGFP or NT3 transgene expression following transplantation of hMSC-EGFP, hMSC-NT3 and hMSC-NT3-EGFP in rat spinal cord. While in vivo EGFP mRNA and protein expression by transplanted hMSC-EGFP cells was readily detectable at different time points post-transplantation, in vivo NT3 mRNA expression by hMSC-NT3 cells and in vivo EGFP protein expression by hMSC-NT3-EGFP cells was, respectively, undetectable or declined rapidly between day 1 and 7 post-transplantation. Further investigation revealed that the observed in vivo decline of EGFP protein expression by hMSC-NT3-EGFP cells: (i was associated with a decrease in transgenic NT3-EGFP mRNA expression as suggested following laser capture micro-dissection analysis of hMSC-NT3-EGFP cell transplants at day 1 and day 7 post-transplantation, (ii did not occur when hMSC-NT3-EGFP cells were transplanted subcutaneously, and (iii was reversed upon re-establishment of hMSC-NT3-EGFP cell cultures at 2 weeks post-transplantation. Finally, because we observed a slowly progressing tumour growth following transplantation of all our hMSC cell transplants, we here demonstrate that omitting immune suppressive therapy is sufficient to prevent further tumour growth and to eradicate malignant xenogeneic cell transplants. Conclusion In this study, we

  18. GAC-EPA

    CERN Document Server

    GAC-EPA

    2012-01-01

    Alzheimer Association Switzerland Wednesday, 29th February 2012 - From 6 pm to 8 pm Hotel Mandarin Oriental - Quai Turrettini 1 - Geneva A public conference in English about   Alzheimer’s disease and other dementias   Prof. Dr Gabriel GOLD, Head of the Division of Geriatrics at Geneva University Hospital, will talk about medical topics related to Alzheimer’s disease and other dementias. Elizabeth BOHLER-GOODSHIP, Mayor of Grand-Saconnex and Vice-president of Alzheimer Geneva, will present the section’s services for English-speaking people in the Geneva area. Birgitta MARTENSSON, Executive Director of Alzheimer Switzerland, will highlight some facts and figures about dementia in Switzerland. Drinks and snacks will be served after the presentations, giving the audience the opportunity to talk with the speakers and other members of the Association. Entrance free To help us organize the event, please announce your participation to sophie.courvoisier@a...

  19. Lähme, naised / Tiina Kuuler

    Index Scriptorium Estoniae

    Kuuler, Tiina, 1969-

    2008-01-01

    Muusikasündmustest: "Laulus on Eestit" 8. aug. Hiiumaal Kärdla laululaval, "Päikeseloojangumuusika: Vägilased" 8. aug. Hiiumaa Kõpu tuletornis, "Kaunid kontserdid Käsmus" (8. aug. Johanson ja vennad, 9. aug. Indigolapsed, 10. aug. Jääboiler), näitemäng "Ernesaks ja Oamats" 16.-17. aug. Raasikul Harju-Jaani kirikumõisas, Leigo järvemuusika (8. aug. ürgsed hümnid, Vaiko Eplik ja Eliit, Jimi Tenor & Kabu Kabu, Eivor ja Tallinna Kammerorkester, Chalice'i uue plaadi esitlus), "Promenaad Kadriorus" 14. aug. (promenaadiorkester Peeter Sauli dirigeerimisel ja solistid), rokkooper "Ruja" 15.-17. ja 19., 21.-24. aug. Vanemuise suvelaval, Birgitta festival 8.-16. aug. Pirita kloostris

  20. Mesenchymal stem cells attenuate inflammatory processes in the heart and lung via inhibition of TNF signaling.

    Science.gov (United States)

    Martire, Alessandra; Bedada, Fikru B; Uchida, Shizuka; Pöling, Jochen; Krüger, Marcus; Warnecke, Henning; Richter, Manfred; Kubin, Thomas; Herold, Susanne; Braun, Thomas

    2016-09-01

    Mesenchymal stem cells (MSC) have been used to treat different clinical conditions although the mechanisms by which pathogenetic processes are affected are still poorly understood. We have previously analyzed the homing of bone marrow-derived MSC to diseased tissues characterized by a high degree of mononuclear cell infiltration and postulated that MSC might modulate inflammatory responses. Here, we demonstrate that MSC mitigate adverse tissue remodeling, improve organ function, and extend lifespan in a mouse model of inflammatory dilative cardiomyopathy (DCM). Furthermore, MSC attenuate Lipopolysaccharide-induced acute lung injury indicating a general role in the suppression of inflammatory processes. We found that MSC released sTNF-RI, which suppressed activation of the NFκBp65 pathway in cardiomyocytes during DCM in vivo. Substitution of MSC by recombinant soluble TNF-R partially recapitulated the beneficial effects of MSC while knockdown of TNF-R prevented MSC-mediated suppression of the NFκBp65 pathway and improvement of tissue pathology. We conclude that sTNF-RI is a major part of the paracrine machinery by which MSC effect local inflammatory reactions. PMID:27435289

  1. Longitudinal Cell Tracking and Simultaneous Monitoring of Tissue Regeneration after Cell Treatment of Natural Tendon Disease by Low-Field Magnetic Resonance Imaging

    Directory of Open Access Journals (Sweden)

    Dagmar Berner

    2016-01-01

    Full Text Available Treatment of tendon disease with multipotent mesenchymal stromal cells (MSC is a promising option to improve tissue regeneration. To elucidate the mechanisms by which MSC support regeneration, longitudinal tracking of MSC labelled with superparamagnetic iron oxide (SPIO by magnetic resonance imaging (MRI could provide important insight. Nine equine patients suffering from tendon disease were treated with SPIO-labelled or nonlabelled allogeneic umbilical cord-derived MSC by local injection. Labelling of MSC was confirmed by microscopy and MRI. All animals were subjected to clinical, ultrasonographical, and low-field MRI examinations before and directly after MSC application as well as 2, 4, and 8 weeks after MSC application. Hypointense artefacts with characteristically low signal intensity were identified at the site of injection of SPIO-MSC in T1- and T2∗-weighted gradient echo MRI sequences. They were visible in all 7 cases treated with SPIO-MSC directly after injection, but not in the control cases treated with nonlabelled MSC. Furthermore, hypointense artefacts remained traceable within the damaged tendon tissue during the whole follow-up period in 5 out of 7 cases. Tendon healing could be monitored at the same time. Clinical and ultrasonographical findings as well as T2-weighted MRI series indicated a gradual improvement of tendon function and structure.

  2. Estrogenic effects of marijuana smoke condensate and cannabinoid compounds

    International Nuclear Information System (INIS)

    Chronic exposure to marijuana produces adverse effects on the endocrine and reproductive systems in humans; however, the experimental evidence for this presented thus far has not been without controversy. In this study, the estrogenic effect of marijuana smoke condensate (MSC) was evaluated using in vitro bioassays, viz., the cell proliferation assay, the reporter gene assay, and the ER competitive binding assay. The results of these assays were compared with those of three major cannabinoids, i.e., THC, CBD, and CBN. The estrogenic effect of MSC was further confirmed by the immature female rat uterotrophic assay. MSC stimulated the estrogenicity related to the ER-mediated pathway, while neither THC, CBD, nor CBN did. Moreover, treatment with 10 and 25 mg/kg MSC induced significant uterine response, and 10 mg/kg MSC resulted in an obvious change in the uterine epithelial cell appearance. MSC also enhanced the IGFBP-1 gene expression in a dose-dependent manner. To identify the constituents of MSC responsible for its estrogenicity, the MSC fractionated samples were examined using another cell proliferation assay, and the estrogenic active fraction was analyzed using GC-MS. In the organic acid fraction that showed the strongest estrogenic activity among the seven fractions of MSC, phenols were identified. Our results suggest that marijuana abuse is considered an endocrine-disrupting factor. Furthermore, these results suggest that the phenolic compounds contained in MSC play a role in its estrogenic effect

  3. Reduced reactivation from dormancy but maintained lineage choice of human mesenchymal stem cells with donor age.

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    Verena Dexheimer

    Full Text Available UNLABELLED: Mesenchymal stem cells (MSC are promising for cell-based regeneration therapies but up to date it is still controversial whether their function is maintained throughout ageing. Aim of this study was to address whether frequency, activation in vitro, replicative function, and in vitro lineage choice of MSC is maintained throughout ageing to answer the question whether MSC-based regeneration strategies should be restricted to younger individuals. MSC from bone marrow aspirates of 28 donors (5-80 years were characterized regarding colony-forming unit-fibroblast (CFU-F numbers, single cell cloning efficiency (SSCE, osteogenic, adipogenic and chondrogenic differentiation capacity in vitro. Alkaline phosphatase (ALP activity, mineralization, Oil Red O content, proteoglycan- and collagen type II deposition were quantified. While CFU-F frequency was maintained, SSCE and early proliferation rate decreased significantly with advanced donor age. MSC with higher proliferation rate before start of induction showed stronger osteogenic, adipogenic and chondrogenic differentiation. MSC with high osteogenic capacity underwent better chondrogenesis and showed a trend to better adipogenesis. Lineage choice was, however, unaltered with age. CONCLUSION: Ageing influenced activation from dormancy and replicative function of MSC in a way that it may be more demanding to mobilize MSC to fast cell growth at advanced age. Since fast proliferation came along with high multilineage capacity, the proliferation status of expanded MSC rather than donor age may provide an argument to restrict MSC-based therapies to certain individuals.

  4. Stem cell technology for bone regeneration: current status and potential applications

    Directory of Open Access Journals (Sweden)

    Asatrian G

    2015-02-01

    Full Text Available Greg Asatrian,1 Dalton Pham,1,2 Winters R Hardy,3 Aaron W James,1–3 Bruno Peault3,4 1Dental and Craniofacial Research Institute and Section of Orthodontics, School of Dentistry, 2Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, 3UCLA/Orthopaedic Hospital Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, Los Angeles, CA, USA; 4Medical Research Council Centre for Regenerative Medicine, Edinburgh, Scotland, UK Abstract: Continued improvements in the understanding and application of mesenchymal stem cells (MSC have revolutionized tissue engineering. This is particularly true within the field of skeletal regenerative medicine. However, much remains unknown regarding the native origins of MSC, the relative advantages of different MSC populations for bone regeneration, and even the biologic safety of such unpurified, grossly characterized cells. This review will first summarize the initial discovery of MSC, as well as the current and future applications of MSC in bone tissue engineering. Next, the relative advantages and disadvantages of MSC isolated from distinct tissue origins are debated, including the MSC from adipose, bone marrow, and dental pulp, among others. The perivascular origin of MSC is next discussed. Finally, we briefly comment on pluripotent stem cell populations and their possible application in bone tissue engineering. While continually expanding, the field of MSC-based bone tissue engineering and regeneration shows potential to become a clinical reality in the not-so-distant future.Keywords: mesenchymal stem cell, pericyte, bone tissue engineering, MSC, ASC, DMSC

  5. Tissue Regeneration of the Vocal Fold Using Bone Marrow Mesenchymal Stem Cells and Synthetic Extracellular Matrix Injections in Rats

    Science.gov (United States)

    Johnson, Beatriz Helena Quinchia; Fox, Ryan; Chen, Xia; Thibeault, Susan

    2009-01-01

    Objective To determine the effectiveness of bone marrow mesenchymal stem cell (BM-MSC) transplantation in isolation or within a synthetic extracellular matrix (sECM) for tissue regeneration of the scarred vocal fold lamina propria. Methods In vitro stability and compatibility of mouse BM-MSC embedded in sECM was assessed by flow cytometry detection of BM-MSC marker expression and proliferation. Eighteen rats were subjected to vocal fold injury bilaterally, followed by one month post-treatment with unilateral injections of saline or sECM hydrogel (Extracel), GFP-mouse BM-MSC or BM-MSC suspended in sECM. Outcomes measured one month after treatment included procollagen-III, fibronectin, hyaluronan synthase-III (HAS3), hyaluronidase (HYAL3), smooth muscle actin (SMA) and transforming growth factor-beta 1(TGF-β1) mRNA expression. The persistence of GFP BM-MSC, proliferation, apoptosis and myofibroblast differentiation was assessed by immunofluorescence. Results BM-MSC grown in vitro within sECM express Sca-1, are positive for hyaluronan receptor CD44 and continue to proliferate. In the in vivo study, groups injected with BM-MSC had detectable GFP-labeled BM-MSC remaining, showed proliferation and low apoptotic or myofibroblast markers compared to the contralateral side. Embedded BM-MSC in sECM group exhibited increased levels of procollagen III, fibronectin and TGF-β1. BM-MSC within sECM downregulated the expression of SMA compared to BM-MSC alone, exhibited upregulation of HYAL3 and no change in HAS3 compared to saline. Conclusions Treatment of vocal fold scarring with BM-MSC injected in a sECM displayed the most favorable outcomes in ECM production, hyaluronan metabolism, myofibroblast differentiation and production of TGF-β1. Furthermore, the combined treatment had no detectable cytotoxicity and preserved local cell proliferation. PMID:20131370

  6. Cardiomyocyte protection by GATA-4 gene engineered mesenchymal stem cells is partially mediated by translocation of miR-221 in microvesicles.

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    Bin Yu

    Full Text Available INTRODUCTION: microRNAs (miRs, a novel class of small non-coding RNAs, are involved in cell proliferation, differentiation, development, and death. In this study, we found that miR-221 translocation by microvesicles (MVs plays an important role in cardioprotection mediated by GATA-4 overexpressed mesenchymal stem cells (MSC. METHODS AND RESULTS: Adult rat bone marrow MSC and neonatal rat ventricle cardiomyocytes (CM were harvested as primary cultures. MSC were transduced with GATA-4 (MSC(GATA-4 using the murine stem cell virus (pMSCV retroviral expression system. Empty vector transfection was used as a control (MSC(Null. The expression of miRs was assessed by real-time PCR and localized using in situ hybridization (ISH. MVs collected from MSC cultures were characterized by expression of CD9, CD63, and HSP70, and photographed with electron microscopy. Cardioprotection during hypoxia afforded by conditioned medium (CdM from MSC cultures was evaluated by lactate dehydrogenase (LDH release, MTS uptake by CM, and caspase 3/7 activity. Expression of miR-221/222 was significantly higher in MSC than in CM and miR-221 was upregulated in MSC(GATA-4. MSC overexpression of miR-221 significantly enhanced cardioprotection by reducing the expression of p53 upregulated modulator of apoptosis (PUMA. Moreover, expression of PUMA was significantly decreased in CM co-cultured with MSC. MVs derived from MSC expressed high levels of miR-221, and were internalized quickly by CM as documented in images obtained from a Time-Lapse Imaging System. CONCLUSIONS: Our results demonstrate that cardioprotection by MSC(GATA-4 may be regulated in part by a transfer of anti-apoptotic miRs contained within MVs.

  7. Effect of human adipose tissue-derived mesenchymal-stem-cell bioactive materials on porcine embryo development.

    Science.gov (United States)

    Park, Hyo-Young; Kim, Eun-Young; Lee, Seung-Eun; Choi, Hyun-Yong; Moon, Jeremiah Jiman; Park, Min-Jee; Son, Yeo-Jin; Lee, Jun-Beom; Jeong, Chang-Jin; Lee, Dong-Sun; Riu, Key-Jung; Park, Se-Pill

    2013-12-01

    Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) secrete bioactive materials that are beneficial for tissue repair and regeneration. In this study, we characterized human hAT-MSC bioactive material (hAT-MSC-BM), and examined the effect of hAT-MSC-BM on porcine embryo development. hAT-MSC-BM was enriched with several growth factors and cytokines, including fibroblast growth factor 2 (FGF2), vascular endothelial growth factor A (VEGFA), and interleukin 6 (IL6). Among the various concentrations and days of treatment tested, 10% hAT-MSC-BM treatment beginning on culture Day 4 provided the best environment for the in vitro growth of parthenogenetic porcine embryos. While the addition of 10% fetal bovine serum (FBS) increased the hatching rate and the total cell number of parthenogenetic porcine embryos compared with the control and hAT-MSC culture medium group, the best results were from the group cultured with 10% hAT-MSC-BM. Mitochondrial activity was also higher in the 10% hAT-MSC-BM-treated group. Moreover, the relative mRNA expression levels of development and anti-apoptosis genes were significantly higher in the 10% hAT-MSC-BM-treated group than in control, hAT-MSC culture medium, or 10% FBS groups, whereas the transcript abundance of an apoptosis gene was slightly lower. Treatment with 10% hAT-MSC-BM starting on Day 4 also improved the development rate and the total cell number of in vitro-fertilized embryos. This is the first report on the benefits of hAT-MSC-BM in a porcine embryo in vitro culture system. We conclude that hAT-MSC-BM is a new, alternative supplement that can improve the development of porcine embryos during both parthenogenesis and fertilization in vitro.

  8. Mechano-growth factor induces migration of rat mesenchymal stem cells by altering its mechanical properties and activating ERK pathway

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jiamin; Wu, Kewen; Lin, Feng; Luo, Qing; Yang, Li; Shi, Yisong [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Song, Guanbin, E-mail: song@cqu.edu.cn [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Sung, Kuo-Li Paul [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412 (United States)

    2013-11-08

    Highlights: •MGF induced the migration of rat MSC in a concentration-dependent manner. •MGF enhanced the mechanical properties of rMSC in inducing its migration. •MGF activated the ERK 1/2 signaling pathway of rMSC in inducing its migration. •rMSC mechanics may synergy with ERK 1/2 pathway in MGF-induced rMSC migration. -- Abstract: Mechano-growth factor (MGF) generated by cells in response to mechanical stimulation has been identified as a mechano effector molecule, playing a key role in regulating mesenchymal stem cell (MSC) function, including proliferation and migration. However, the mechanism(s) underlying how MGF-induced MSC migration occurs is still unclear. In the present study, MGF motivated migration of rat MSCs (rMSCs) in a concentration-dependent manner and optimal concentration of MGF at 50 ng/mL (defined as MGF treatment in this paper) was demonstrated. Notably, enhancement of mechanical properties that is pertinent to cell migration, such as cell traction force and cell stiffness were found to respond to MGF treatment. Furthermore, MGF increased phosphorylation of extracellular signal-regulated kinase (ERK), ERK inhibitor (i.e., PD98059) suppressed ERK phosphorylation, and abolished MGF-induced rMSC migration were found, demonstrating that ERK is involved molecule for MGF-induced rMSC migration. These in vitro evidences of MGF-induced rMSC migration and its direct link to altering rMSC mechanics and activating the ERK pathway, uncover the underlying biomechanical and biological mechanisms of MGF-induced rMSC migration, which may help find MGF-based application of MSC in clinical therapeutics.

  9. Mitochondrial Transfer via Tunneling Nanotubes is an Important Mechanism by Which Mesenchymal Stem Cells Enhance Macrophage Phagocytosis in the In Vitro and In Vivo Models of ARDS

    Science.gov (United States)

    Jackson, Megan V.; Morrison, Thomas J.; Doherty, Declan F.; McAuley, Daniel F.; Matthay, Michael A.; Kissenpfennig, Adrien; O'Kane, Cecilia M.

    2016-01-01

    Abstract Mesenchymal stromal cells (MSC) have been reported to improve bacterial clearance in preclinical models of Acute Respiratory Distress Syndrome (ARDS) and sepsis. The mechanism of this effect is not fully elucidated yet. The primary objective of this study was to investigate the hypothesis that the antimicrobial effect of MSC in vivo depends on their modulation of macrophage phagocytic activity which occurs through mitochondrial transfer. We established that selective depletion of alveolar macrophages (AM) with intranasal (IN) administration of liposomal clodronate resulted in complete abrogation of MSC antimicrobial effect in the in vivo model of Escherichia coli pneumonia. Furthermore, we showed that MSC administration was associated with enhanced AM phagocytosis in vivo. We showed that direct coculture of MSC with monocyte‐derived macrophages enhanced their phagocytic capacity. By fluorescent imaging and flow cytometry we demonstrated extensive mitochondrial transfer from MSC to macrophages which occurred at least partially through tunneling nanotubes (TNT)‐like structures. We also detected that lung macrophages readily acquire MSC mitochondria in vivo, and macrophages which are positive for MSC mitochondria display more pronounced phagocytic activity. Finally, partial inhibition of mitochondrial transfer through blockage of TNT formation by MSC resulted in failure to improve macrophage bioenergetics and complete abrogation of the MSC effect on macrophage phagocytosis in vitro and the antimicrobial effect of MSC in vivo. Collectively, this work for the first time demonstrates that mitochondrial transfer from MSC to innate immune cells leads to enhancement in phagocytic activity and reveals an important novel mechanism for the antimicrobial effect of MSC in ARDS. Stem Cells 2016;34:2210–2223 PMID:27059413

  10. Mitochondrial Transfer via Tunneling Nanotubes is an Important Mechanism by Which Mesenchymal Stem Cells Enhance Macrophage Phagocytosis in the In Vitro and In Vivo Models of ARDS.

    Science.gov (United States)

    Jackson, Megan V; Morrison, Thomas J; Doherty, Declan F; McAuley, Daniel F; Matthay, Michael A; Kissenpfennig, Adrien; O'Kane, Cecilia M; Krasnodembskaya, Anna D

    2016-08-01

    Mesenchymal stromal cells (MSC) have been reported to improve bacterial clearance in preclinical models of Acute Respiratory Distress Syndrome (ARDS) and sepsis. The mechanism of this effect is not fully elucidated yet. The primary objective of this study was to investigate the hypothesis that the antimicrobial effect of MSC in vivo depends on their modulation of macrophage phagocytic activity which occurs through mitochondrial transfer. We established that selective depletion of alveolar macrophages (AM) with intranasal (IN) administration of liposomal clodronate resulted in complete abrogation of MSC antimicrobial effect in the in vivo model of Escherichia coli pneumonia. Furthermore, we showed that MSC administration was associated with enhanced AM phagocytosis in vivo. We showed that direct coculture of MSC with monocyte-derived macrophages enhanced their phagocytic capacity. By fluorescent imaging and flow cytometry we demonstrated extensive mitochondrial transfer from MSC to macrophages which occurred at least partially through tunneling nanotubes (TNT)-like structures. We also detected that lung macrophages readily acquire MSC mitochondria in vivo, and macrophages which are positive for MSC mitochondria display more pronounced phagocytic activity. Finally, partial inhibition of mitochondrial transfer through blockage of TNT formation by MSC resulted in failure to improve macrophage bioenergetics and complete abrogation of the MSC effect on macrophage phagocytosis in vitro and the antimicrobial effect of MSC in vivo. Collectively, this work for the first time demonstrates that mitochondrial transfer from MSC to innate immune cells leads to enhancement in phagocytic activity and reveals an important novel mechanism for the antimicrobial effect of MSC in ARDS. Stem Cells 2016;34:2210-2223.

  11. Aspectos sociopsicológicos del medio laboral asociados a la autoestima de personas que viven con VIHn Notificaciones Ver (159 Administrar  Contenido de la revista Herramientas del artículo Imprimir este artículo Metadatos de indexación Cómo citar un elemento Publicar un comentario Información Para los lectores/as Para los autores/as Para los bibliotecarios/as Acerca de los autores/as Lic. Nielvis de la Caridad Senra Pérez MSc. Mailé Contrera Betarte MSc. Yexenia Martín Chávez Encuéntranos en... Inicio / Aspectos sociopsicológicos del medio laboral asociados a la autoestima de personas que viven con VIH

    Directory of Open Access Journals (Sweden)

    Lic. Nielvis de la Caridad Senra Pérez

    2016-06-01

    Full Text Available La presente investigación consiste en un estudio cuali-cuantitativo, no experimental, que comienza siendo descriptivo y continúa siendo correlacional, describiendo y relacionando las variables: autoestima, apoyo social y conocimiento que poseen los directivos y trabajadores sobre el Virus de Inmunodeficiencia Humana. Persiguiendo como objetivo determinar la posible asociación entre los aspectos sociopsicológicos del medio laboral con la autoestima de las personas que viven con el virus. Para la realización del mismo se emplean métodos del nivel teórico, empírico y estadístico. La selección de la muestra se hace coincidir con el universo (todas las personas de Rodas que viven con el Virus de Inmunodeficiencia Humana, que se encuentran laborando por ser este uno de los municipios que más casos aporta en la provincia de Cienfuegos. Se emplean diferentes técnicas para la recogida de datos como son: entrevista en profundidad, semiestructurada, observación participante, cuestionario de apoyo social e inventario de autoestima de Coopersmith. Los resultados se muestran en tablas y figuras, así como se hace un análisis porcentual y se realiza la valoración estadística mediante la correlación de Spearman.

  12. Lignin-rich biomass of cotton by-products for biorefineries via pyrolysis.

    Science.gov (United States)

    Chen, Jiao; Liang, Jiajin; Wu, Shubin

    2016-10-01

    Pyrolysis was demonstrated to investigate the thermal decomposition characteristics and potential of lignin-rich cotton by-products cotton exocarp (CE) and spent mushroom substrate consisted of cotton by-products (MSC) for biorefineries. The chemical component and structure alteration of CE and MSC was found to affect their thermochemical behaviors. The bio-oil yield from CE was 58.13wt% while the maximum yield from MSC was 45.01% at 600°C. The phenolic compounds obtained from CE and MSC were 33.9% and 39.2%, respectively. The yield of acetic acid from MSC between 400 and 600°C was about 30-38% lower than that from CE, which suggests the high quality of bio-oil was obtained. Biochar from MSC via slow pyrolysis had a high mass yield (44.38wt%) with well-developed pore structure. PMID:27393830

  13. Architecture and Function of Mechanosensitive Membrane Protein Lattices

    CERN Document Server

    Kahraman, Osman; Klug, William S; Haselwandter, Christoph A

    2016-01-01

    Experiments have revealed that membrane proteins can form two-dimensional clusters with regular translational and orientational protein arrangements, which may allow cells to modulate protein function. However, the physical mechanisms yielding supramolecular organization and collective function of membrane proteins remain largely unknown. Here we show that bilayer-mediated elastic interactions between membrane proteins can yield regular and distinctive lattice architectures of protein clusters, and may provide a link between lattice architecture and lattice function. Using the mechanosensitive channel of large conductance (MscL) as a model system, we obtain relations between the shape of MscL and the supramolecular architecture of MscL lattices. We predict that the tetrameric and pentameric MscL symmetries observed in previous structural studies yield distinct lattice architectures of MscL clusters and that, in turn, these distinct MscL lattice architectures yield distinct lattice activation barriers. Our res...

  14. Prenatal transplantation of mesenchymal stem cells to treat osteogenesis imperfecta.

    Directory of Open Access Journals (Sweden)

    Jerry KY Chan

    2014-10-01

    Full Text Available Osteogenesis Imperfecta (OI can be a severe disorder that can be diagnosed before birth. Transplantation of mesenchymal stem cells (MSC has the potential to improve the bone structure, growth and fracture healing. In this review we give an introduction to OI and MSC, and the basis for prenatal and postnatal transplantation in OI. We also summarize the two patients with OI who has received prenatal and postnatal transplantation of MSC.The findings suggest that prenatal transplantation of allogeneic MSC in OI is safe. The cell therapy is of likely clinical benefit with improved linear growth, mobility and reduced fracture incidence. Unfortunately, the effect is transient. For this reason postnatal booster infusions using same-donor MSC have been performed with clinical benefit, and without any adverse events.So far there is limited experience in this specific field and proper studies are required to accurately conclude on clinical benefits of MSC transplantation to treat OI.

  15. [Exosomes Derived from Mesenchymal Stem Cells--the Future Ideal Vector of Biological Therapy].

    Science.gov (United States)

    Zhang, Juan; Shi, Jing-Shu; Li, Jian

    2015-08-01

    MSC-exosomes are homogeneous menbrane vesicles with diameter 40-100 nm, derived from mesenchymal stem cells at physiological or pathology conditions. MSC-exosomes contain a great quantity and a wide variety of bioactive substances, such as proteins and miRNA. MSC-exosomes transfer bioactive substances to recipient cells to affect their functions through membrane fusion or endocytosis, which like the storage pools of signal vehicles for cell-to-cell comunication in vivo. MSC-exosomes can mimic the beneficial effect of MSC treatment, such as the promotion of tissue repair or the immune regulation. The biological property and functions of MSC-exosomes are reviwed in this article. PMID:26314469

  16. Hepatoma SK Hep-1 cells exhibit characteristics of oncogenic mesenchymal stem cells with highly metastatic capacity.

    Directory of Open Access Journals (Sweden)

    Jong Ryeol Eun

    Full Text Available BACKGROUND: SK Hep-1 cells (SK cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, SK cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Here, we characterized SK cells and its tumourigenicity. METHODS AND PRINCIPAL FINDINGS: We found that classical mesenchymal stem cell (MSC markers were presented on SK cells, but endothelial marker CD31, hematopoietic markers CD34 and CD45 were negative. SK cells are capable of differentiate into adipocytes and osteoblasts as adipose-derived MSC (Ad-MSC and bone marrow-derived MSC (BM-MSC do. Importantly, a single SK cell exhibited a substantial tumourigenicity and metastatic capacity in immunodefficient mice. Metastasis not only occurred in circulating organs such as lung, liver, and kidneys, but also in muscle, outer abdomen, and skin. SK cells presented greater in vitro invasive capacity than those of Ad-MSC and BM-MSC. The xenograft cells from subcutaneous and metastatic tumors exhibited a similar tumourigenicity and metastatic capacity, and showed the same relatively homogenous population with MSC characteristics when compared to parental SK cells. SK cells could unlimitedly expand in vitro without losing MSC characteristics, its tumuorigenicity and metastatic capacity, indicating that SK cells are oncogenic MSC with enhanced self-renewal capacity. We believe that this is the first report that human MSC appear to be transformed into cancer stem cells (CSC, and that their derivatives also function as CSCs. CONCLUSION: Our findings demonstrate that SK cells represent a transformation mechanism of normal MSC into an enhanced self-renewal CSC with metastasis capacity, SK cells and their xenografts represent a same relative homogeneity of CSC with substantial metastatic capacity. Thus, it represents a

  17. [Distribution of compact bone mesenchymal stem cells in lung tissue and bone marrow of mouse].

    Science.gov (United States)

    Wang, Rui-Ping; Wu, Ren-Na; Guo, Yu-Qing; Zhang, Bin; Chen, Hu

    2014-02-01

    This study was aimed to investigate the distribution of compact bone mesenchymal stem cells(MSC) marked with lentiviral plasmid pGC FU-RFP-LV in lung tissue and bone marrow of mouse. The MSC were infected by lentivirus with infection efficiency 78%, the infected MSC were injected into BALB/c mice via tail veins in concentration of 1×10(6) /mouse. The mice were randomly divided into 4 group according to 4 time points as 1, 2, 5 and 7 days. The lung tissue and bone marrow were taken and made of frozen sections and smears respectively in order to observed the distributions of MSC. The results indicated that the lentiviral infected MSC displayed phenotypes and biological characteristics which conformed to MSC by immunophenotyping analysis and induction differentiation detection. After the MSC were infected with optimal viral titer MOI = 50, the cell growth no significantly changed; the fluorescent microscopy revealed that the distributions of MSC in bone marrow on day 1, 2, 5 and 7 were 0.50 ± 0.20, 0.67 ± 0.23, 0.53 ± 0.14, 0.33 ± 0.16; those in lung tissue were 0.55 ± 0.15, 0.47 ± 0.13, 0.29 ± 0.13, 0.26 ± 0.08. It is concluded that the distribution of MSC in lung tissue reaches a peak on day 1, while distribution of MSC in bone marrow reaches a peak on day 2. The distribution of mouse MSC relates with RFP gene expression and implantation of MSC in lung tissue and bone marrow.

  18. Toepassingen van mesenchymale stamcellen bij het paard: huidige stand van zaken

    OpenAIRE

    De Schauwer, Catharina; Meyer, Evelyne; Van de Walle, Gerlinde; Van Soom, Ann

    2013-01-01

    Mesenchymal stem cells (MSC) are adult stem cells, which are capable of self-renewal and restricted differentiation into multiple organ-specific cell types. The significant therapeutic potential of MSC arises from their ability to promote tissue regeneration, prevent pathological scar formation, modulate immune responses and regulate inflammation. At present, MSC are mainly used in veterinary medicine to treat musculoskeletal injuries. Nevertheless, they may as well play a role in the trea...

  19. Experiences with mechatronics education at the University of Twente

    OpenAIRE

    Amerongen, van, W.E.

    2003-01-01

    This paper describes the experiences with a number of variants of mechatronic programmes offered by the University of Twente since 1989. Mechatronics education took place in a two-year mechatronic designer programme, in specialisations in Electrical and Mechanical Engineering and in an international MSc programme. In the new European BSc/MSc structure the University of Twente will offer an MSc mechatronics where the course language will be English. There have been large mechatronic projects, ...

  20. Instant stem cell therapy: Characterization and concentration of human mesenchymal stem cells in vitro

    OpenAIRE

    Kasten, P; I Beyen; Egermann, M.; AJ Suda; AA Moghaddam; Zimmermann, G; R Luginbühl

    2008-01-01

    In regenerative medicine, there is an approach to avoid expansion of the mesenchymal stem cell (MSC) before implantation. The aim of this study was to compare methods for instant MSC therapy by use of a portable, automatic and closed system centrifuge that allows for the concentration of MSCs. The main outcome measures were the amount of MSCs per millilitre of bone marrow (BM), clusters of differentiation (CD), proliferation and differentiation capacities of the MSC. A volume reduction protoc...

  1. Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype

    OpenAIRE

    Clark, Kaitlin C.; Kol, Amir; Shahbenderian, Salpi; Granick, Jennifer L.; Walker, Naomi J.; Borjesson, Dori L.

    2015-01-01

    Mesenchymal stem cell (MSC) therapy is being increasingly used to treat dogs and horses with naturally-occurring diseases. However these animals also serve as critical large animal models for ongoing translation of cell therapy products to the human market. MSC manufacture for clinical use mandates improvement in cell culture systems to meet demands for higher MSC numbers and removal of xeno-proteins (i.e. fetal bovine serum, FBS). While serum-free media (SFM) is commercially available, its a...

  2. Mesenchymal stromal cells reset the scatter factor system and cytokine network in experimental kidney transplantation

    OpenAIRE

    Gregorini, Marilena; Bosio, Francesca; Rocca, Chiara; Corradetti, Valeria; Valsania, Teresa; Pattonieri, Eleonora Francesca; Esposito, Pasquale; Bedino, Giulia; Collesi, Chiara; Libetta, Carmelo; Frassoni, Francesco; Canton, Antonio Dal; Rampino, Teresa

    2014-01-01

    Background In former studies we showed in a rat model of renal transplantation that Mesenchymal Stromal Cells (MSC) prevent acute rejection in an independent way of their endowing in the graft. In this study we investigated whether MSC operate by resetting cytokine network and Scatter Factor systems, i.e. Hepatocyte Growth Factor (HGF), Macrophage Stimulating Protein (MSP) and their receptors Met and RON, respectively. Methods MSC were injected into the renal artery soon after reperfusion. Co...

  3. Mesenchymal Stem Cell Attenuates Neutrophil-predominant Inflammation and Acute Lung Injury in an In Vivo Rat Model of Ventilator-induced Lung Injury

    OpenAIRE

    Tian-Shun Lai; Zhi-Hong Wang; Shao-Xi Cai

    2015-01-01

    Background: Subsequent neutrophil (polymorphonuclear neutrophil [PMN])-predominant inflammatory response is a predominant feature of ventilator-induced lung injury (VILI), and mesenchymal stem cell (MSC) can improve mice survival model of endotoxin-induced acute lung injury, reduce lung impairs, and enhance the repair of VILI. However, whether MSC could attenuate PMN-predominant inflammatory in the VILI is still unknown. This study aimed to test whether MSC intervention could attenuate the PM...

  4. Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix

    OpenAIRE

    Tapp, Hazel; Deepe, Ray; Ingram, Jane A; Kuremsky, Marshall; Hanley, Edward N; Gruber, Helen E.

    2008-01-01

    Introduction Adult mesenchymal stem cell therapy has a potential application in the biological treatment of disc degeneration. Our objectives were: to direct adipose-derived mesenchymal stem cells (AD-MSC) from the sand rat to produce a proteoglycan and collagen type I extracellular matrix (ECM) rich in known ECM components of the annulus fibrosis of disc; and to stimulate proteoglycan production by co-culture of human annulus cells with AD-MSC. Methods AD-MSC were isolated and characterised ...

  5. Immunomodulation by Mesenchymal Stem Cells in Veterinary Species

    OpenAIRE

    Carrade, Danielle D.; Borjesson, Dori L.

    2013-01-01

    Mesenchymal stem cells (MSC) are adult-derived multipotent stem cells that have been derived from almost every tissue. They are classically defined as spindle-shaped, plastic-adherent cells capable of adipogenic, chondrogenic, and osteogenic differentiation. This capacity for trilineage differentiation has been the foundation for research into the use of MSC to regenerate damaged tissues. Recent studies have shown that MSC interact with cells of the immune system and modulate their function. ...

  6. Densification Model for Porous Metallic Powder Materials

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A new mechanical model for powder metallurgy compaction is presented. In this model, various amount of voids can be introduced into a continuous solid, therefore porosity can be conveniently controlled. The elastic-plastic finite element method was used to analyze the sintered powder material. The model was used to simulate compressing of a sintered cylinder. MSC.Marc of MSC. Software Corporation was applied here, and the sintered powder model was built in MSC.Mentat. The sintered cylindrical powder metallu...

  7. A molecular classification of human mesenchymal stromal cells

    OpenAIRE

    Rohart, Florian; Mason, Elizabeth A.; Matigian, Nicholas; Mosbergen, Rowland; Korn, Othmar; Chen, Tyrone; Butcher, Suzanne; Patel, Jatin; Atkinson, Kerry; Khosrotehrani, Kiarash; Fisk, Nicholas M.; Lê Cao, Kim-Anh; Wells, Christine A

    2016-01-01

    Mesenchymal stromal cells (MSC) are widely used for the study of mesenchymal tissue repair, and increasingly adopted for cell therapy, despite the lack of consensus on the identity of these cells. In part this is due to the lack of specificity of MSC markers. Distinguishing MSC from other stromal cells such as fibroblasts is particularly difficult using standard analysis of surface proteins, and there is an urgent need for improved classification approaches. Transcriptome profiling is commonl...

  8. Long-Duration Three-Dimensional Spheroid Culture Promotes Angiogenic Activities of Adipose-Derived Mesenchymal Stem Cells

    OpenAIRE

    Lee, Jun Hee; Han, Yong-Seok; Lee, Sang Hun

    2016-01-01

    Mesenchymal stem cells (MSCs) offer significant therapeutic promise for various regenerative therapies. However, MSC-based therapy for injury exhibits low efficacy due to the pathological environment in target tissues and the differences between in vitro and in vivo conditions. To address this issue, we developed adipose-derived MSC spheroids as a novel delivery method to preserve the stem cell microenvironment. MSC spheroids were generated by suspension culture for 3 days, and their sizes in...

  9. The redesign of the medical informatics master of science course at the University of Amsterdam.

    Science.gov (United States)

    Jaspers, Monique W M; Hasman, Arie

    2007-10-11

    The University of Amsterdam redesigned its former 4 years Medical Informatics university program into a Dutch 3 years BSc program and a 2 years English MSc program. The new MSc program is aimed at (international) baccalaureates in medical informatics, computer science, medicine, health sciences, and biology. Besides, health care professionals or professionals with a background in computer science may enter the program. We present our new MSc program shortly.

  10. Mesenchymal stem cell-conditioned medium prevents radiation-induced liver injury by inhibiting inflammation and protecting sinusoidal endothelial cells

    International Nuclear Information System (INIS)

    Current management of radiation-induced liver injury is limited. Sinusoidal endothelial cell (SEC) apoptosis and inflammation are considered to be initiating events in hepatic damage. We hypothesized that mesenchymal stem cells (MSCs) possess anti-apoptotic and anti-inflammatory actions during hepatic irradiation, acting via paracrine mechanisms. This study aims to examine whether MSC-derived bioactive components are protective against radiation-induced liver injury in rats. MSC-conditioned medium (MSC-CM) was generated from rat bone marrow–derived MSCs. The effect of MSC-CM on the viability of irradiated SECs was examined by flow cytometric analysis. Activation of the Akt and ERK pathways was analyzed by western blot. MSC-CM was also delivered to Sprague–Dawley rats immediately before receiving liver irradiation, followed by testing for pathological features, changes in serum hyaluronic acid, ALT, and inflammatory cytokine levels, and liver cell apoptosis. MSC-CM enhanced the viability of irradiated SECs in vitro and induced Akt and ERK phosphorylation in these cells. Infusion of MSC-CM immediately before liver irradiation provided a significant anti-apoptotic effect on SECs and improved the histopathological features of injury in the irradiated liver. MSC-CM also reduced the secretion and expression of inflammatory cytokines and increased the expression of anti-inflammatory cytokines. MSC-derived bioactive components could be a novel therapeutic approach for treating radiation-induced liver injury. (author)

  11. Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells

    Directory of Open Access Journals (Sweden)

    Fernando Antonio Fierro

    2015-10-01

    Full Text Available Many therapies using mesenchymal stem cells (MSC rely on their ability to produce and release paracrine signals with chemotactic and pro-angiogenic activity. These characteristics, however, are mostly studied under standard in vitro culture conditions. In contrast, various novel cell-based therapies imply pre-seeding MSC into bio-artificial scaffolds. Here we describe human bone marrow-derived MSC seeded in Integra matrices, a common type of scaffold for dermal regeneration (SDR. We show and measured the distribution of MSC within the SDR, where cells clearly establish physical interactions with the scaffold, exhibiting constant metabolic activity for at least 15 days. In the SDR, MSC secrete VEGF and SDF-1 and induce transwell migration of CD34+ hematopoietic/endothelial progenitor cells, which is inhibited in the presence of a CXCR4/SDF-1 antagonist. MSC in SDR respond to hypoxia by altering levels of angiogenic signals such as Angiogenin, Serpin-1, uPA and IL-8. Finally, we show that MSC-containing SDR that have been pre-incubated in hypoxia show higher infiltration of endothelial cells after implantation into immune deficient mice. Our data show that MSC are fully functional ex vivo when implanted into SDR. In addition, our results strongly support the notion of hypoxic pre-conditioning MSC-containing SDR, in order to promote angiogenesis in the wounds.

  12. Interfacing Sca-1pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry

    Directory of Open Access Journals (Sweden)

    G. Forte

    2009-01-01

    Full Text Available An immortalized murine mesenchymal stem cell line (mTERT-MSC enriched for Linneg/Sca-1pos fraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation. Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events. Thus, mTERT-MSC have been cultured onto PLA films, 30 and 100 μm PLA microbeads, and onto unpressed and pressed HYAFF-11 scaffolds. While the cells adhered preserving their morphology on PLA films, clusters of mTERT-MSC were detected on PLA beads and unpressed fibrous scaffolds. Finally, mTERT-MSC were not able to colonize the inner layers of pressed HYAFF-11. Nevertheless, such cell line displayed the ability to preserve Sca-1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.

  13. Good manufacturing practice-compliant animal-free expansion of human bone marrow derived mesenchymal stroma cells in a closed hollow-fiber-based bioreactor.

    Science.gov (United States)

    Nold, Philipp; Brendel, Cornelia; Neubauer, Andreas; Bein, Gregor; Hackstein, Holger

    2013-01-01

    Mesenchymal stroma cells (MSC) are increasingly recognized for various applications of cell-based therapies such as regenerative medicine or immunomodulatory treatment strategies. Standardized large-scale expansions of MSC under good manufacturing practice (GMP)-compliant conditions avoiding animal derived components are mandatory for further evaluation of these novel therapeutic approaches in clinical trials. We applied a novel automated hollow fiber cell expansion system (CES) for in vitro expansion of human bone marrow derived MSC employing a GMP-compliant culture medium with human platelet lysate (HPL). Between 8 and 32 ml primary bone marrow aspirate were loaded into the hollow fiber CES and cultured for 15-27 days. 2-58 million MSC were harvested after primary culture. Further GMP-compliant cultivation of second passage MSC for 13 days led to further 10-20-fold enrichment. Viability, surface antigen expression, differentiation capacity and immunosuppressive function of MSC cultured in the hollow fiber CES were in line with standard criteria for MSC definition. We conclude that MSC can be enriched from primary bone marrow aspirate in a GMP-conform manner within a closed hollow fiber bioreactor and maintain their T lymphocyte inhibitory capacity. Standardized and reliable conditions for large scale MSC expansion pave the way for safe applications in humans in different therapeutic approaches.

  14. Robust Analysis and Prediction for Integrated Design of Structures (RAPIDS) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Commercially available software suites such as the Automate Structural Optimization System (ASTROS) and MSC/NASTRAN represent the current industry standard in...

  15. Induction of miR-146a by multiple myeloma cells in mesenchymal stromal cells stimulates their pro-tumoral activity.

    Science.gov (United States)

    De Veirman, Kim; Wang, Jinheng; Xu, Song; Leleu, Xavier; Himpe, Eddy; Maes, Ken; De Bruyne, Elke; Van Valckenborgh, Els; Vanderkerken, Karin; Menu, Eline; Van Riet, Ivan

    2016-07-10

    Mutual communication between multiple myeloma (MM) cells and mesenchymal stromal cells (MSC) plays a pivotal role in supporting MM progression. In MM, MSC exhibit a different genomic profile and dysregulated cytokine secretion compared to normal MSC, however the mechanisms involved in these changes are not fully understood. Here, we examined the miRNA changes in human MSC after culture with conditioned medium of MM cells and found 19 dysregulated miRNAs, including upregulated miR-146a. Moreover, exosomes derived from MM cells contained miR-146a and could be transferred into MSC. After overexpressing miR-146a in MSC, secretion of several cytokines and chemokines including CXCL1, IL6, IL-8, IP-10, MCP-1, and CCL-5 was elevated, resulting in the enhancement of MM cell viability and migration. DAPT, an inhibitor of the endogenous Notch pathway, was able to abrogate the miR-146a-induced increase of cytokines in MSC, suggesting the involvement of the Notch pathway. Taken together, our results demonstrate a positive feedback loop between MM cells and MSC: MM cells promote the increase of miR146a in MSC which leads to more cytokine secretion, which in turn favors MM cell growth and migration. PMID:27102001

  16. 75 FR 65642 - National Institute of Mental Health; Notice of Closed Meeting

    Science.gov (United States)

    2010-10-26

    ..., Neuroscience Center, 6001 Executive Boulevard, Rockville, MD 20852. (Telephone Conference Call) Contact Person... Mental Health, NIH, Neuroscience Center, 6001 Executive Boulevard, Room 6132, MSC 9608, Bethesda,...

  17. Human Serum is as Efficient as Fetal Bovine Serum in Supporting Proliferation and Differentiation of Human Multipotent Stromal (Mesenchymal) Stem Cells In Vitro and In Vivo

    DEFF Research Database (Denmark)

    Aldahmash, Abdullah; Haack-Sørensen, Mandana; Al-Nbaheen, May;

    2011-01-01

    . FBS. hMSC-TERT or primary bone marrow derived hMSC induced to osteoblastic or adipocytic differentiation in the presence of HuS or FBS showed comparable levels of gene expression and protein production of osteoblastic markers (CBFA1/Runx2, alkaline phosphastase, collagen type I and osteocalcin...... pose a health risk for patients. METHODS: We carried out a side-by-side comparison of the effects of allogenic pooled human serum (HuS) versus FBS on hMSC proliferation and differentiation in vitro and in vivo. As a model for hMSC, we employed telomerase-immortalized hMSC; hMSC-TERT cell line. RESULTS...... subcutaneously in immune deficient mice. hMSC maintained in HuS vs. FBS formed comparable heterotopic bone. DISCUSSION: Human serum can support proliferation and differentiation of hMSC in vitro and can maintain their bone forming capacity in vivo. The use of human serum in cell cultures of hMSC intended...

  18. Neonatal abstinence syndrome

    Science.gov (United States)

    ... JR, Isemann B, Ward LP, et al. Current management of neonatal abstinence syndrome secondary to ... MD, MSc, IBCLC, Associate Professor of Pediatrics, Division of Neonatology, Medical University of ...

  19. Low blood sugar - newborns

    Science.gov (United States)

    ... evaluating "transitional neonatal hypoglycemia": mechanism and implications for management. J Pediatr. ... Lee, MD, MSc, IBCLC, Associate Professor of Pediatrics, Division of Neonatology, Medical University of ...

  20. Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteins.

    Directory of Open Access Journals (Sweden)

    Melisa A Soland

    Full Text Available BACKGROUND: Mesenchymal stem cells (MSC are promising candidates for cell therapy, as they migrate to areas of injury, differentiate into a broad range of specialized cells, and have immunomodulatory properties. However, MSC are not invisible to the recipient's immune system, and upon in vivo administration, allogeneic MSC are able to trigger immune responses, resulting in rejection of the transplanted cells, precluding their full therapeutic potential. Human cytomegalovirus (HCMV has developed several strategies to evade cytotoxic T lymphocyte (CTL and Natural Killer (NK cell recognition. Our goal is to exploit HCMV immunological evasion strategies to reduce MSC immunogenicity. METHODOLOGY/PRINCIPAL FINDINGS: We genetically engineered human MSC to express HCMV proteins known to downregulate HLA-I expression, and investigated whether modified MSC were protected from CTL and NK attack. Flow cytometric analysis showed that amongst the US proteins tested, US6 and US11 efficiently reduced MSC HLA-I expression, and mixed lymphocyte reaction demonstrated a corresponding decrease in human and sheep mononuclear cell proliferation. NK killing assays showed that the decrease in HLA-I expression did not result in increased NK cytotoxicity, and that at certain NK∶MSC ratios, US11 conferred protection from NK cytotoxic effects. Transplantation of MSC-US6 or MSC-US11 into pre-immune fetal sheep resulted in increased liver engraftment when compared to control MSC, as demonstrated by qPCR and immunofluorescence analyses. CONCLUSIONS AND SIGNIFICANCE: These data demonstrate that engineering MSC to express US6 and US11 can be used as a means of decreasing recognition of MSC by the immune system, allowing higher levels of engraftment in an allogeneic transplantation setting. Since one of the major factors responsible for the failure of allogeneic-donor MSC to engraft is the mismatch of HLA-I molecules between the donor and the recipient, MSC-US6 and MSC-US11

  1. Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential.

    Directory of Open Access Journals (Sweden)

    Barbara Mara Klinkhammer

    Full Text Available Mesenchymal stem cell (MSC transplantation has the potential for organ repair. Nevertheless, some factors might lessen the regenerative potential of MSCs, e.g. donor age or systemic disease. It is thus important to carefully assess the patient's suitability for autologous MSC transplantation. Here we investigated the effects of chronic kidney disease (CKD on MSC function. We isolated bone marrow MSCs from remnant kidney rats (RK with CKD (CKD-RK-MSC and found signs of premature senescence: spontaneous adipogenesis, reduced proliferation capacity, active senescence-associated-β-galactosidase, accumulation of actin and a modulated secretion profile. The functionality of CKD-RK-MSCs in vivo was tested in rats with acute anti-Thy1.1-nephritis, where healthy MSCs have been shown to be beneficial. Rats received healthy MSCs, CKD-RK-MSC or medium by injection into the left renal artery. Kidneys receiving healthy MSCs exhibited accelerated healing of glomerular lesions, whereas CKD-RK-MSC or medium exerted no benefit. The negative influence of advanced CKD/uremia on MSCs was confirmed in a second model of CKD, adenine nephropathy (AD. MSCs from rats with adenine nephropathy (CKD-AD-MSC also exhibited cellular modifications and functional deficits in vivo. We conclude that CKD leads to a sustained loss of in vitro and in vivo functionality in MSCs, possibly due to premature cellular senescence. Considering autologous MSC therapy in human renal disease, studies identifying uremia-associated mechanisms that account for altered MSC function are urgently needed.

  2. Minimum staff complement: safety in numbers

    International Nuclear Information System (INIS)

    Adequate staffing is an essential safety barrier for event mitigation. For this reason, Canadian nuclear power plant (NPP) licences specify the minimum staff complement (MSC), which is the number and qualifications of staff always required on-site. A systematic analysis and its validation form the basis of the MSC. The analysis and validation demonstrate a licensee is able to control, cool and contain the reactor after any credible event. The CNSC published regulatory guidelines for analyzing the basis for essential staff levels, monitoring compliance with these levels, and controlling MSC changes (G-323). Lessons learned from a full-scale MSC analysis are discussed. (author)

  3. Mesenchymal stromal cells protect against caspase 3-mediated apoptosis of CD19(+) peripheral B cells through contact-dependent upregulation of VEGF.

    Science.gov (United States)

    Healy, Marc E; Bergin, Ronan; Mahon, Bernard P; English, Karen

    2015-10-15

    The immune suppressive and anti-inflammatory capabilities of bone marrow-derived mesenchymal stromal cells (MSCs) represent an innovative new tool in regenerative medicine and immune regulation. The potent immune suppressive ability of MSC over T cells, dendritic cells, and natural killer cells has been extensively characterized, however, the effect of MSC on B cell function has not yet been clarified. In this study, the direct effect of MSC on peripheral blood B cell function is defined and the mechanism utilized by MSC in enhancing B cell survival in vitro identified. Human MSC supported the activation, proliferation, and survival of purified CD19(+) B cells through a cell contact-dependent mechanism. These effects were not mediated through B cell activating factor or notch signaling. However, cell contact between MSC and B cells resulted in increased production of vascular endothelial growth factor (VEGF) by MSC facilitating AKT phosphorylation within the B cell and inhibiting caspase 3-mediated apoptosis. Blocking studies demonstrated that this cell contact-dependent effect was not dependent on signaling through CXCR4-CXCL12 or through the epidermal growth factor receptor (EGFR). These results suggest that direct cell contact between MSC and B cells supports B cell viability and function, suggesting that MSC may not represent a suitable therapy for B cell-mediated disease. PMID:26076727

  4. Bone marrow mesenchymal stem cell transplantation combined with perindopril treatment attenuates infarction remodelling in a rat model of acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    WANG Jian-an; LUO Rong-hua; ZHANG Xing; XIE Xiao-jie; HU Xin-yang; HE Ai-na; CHEN Jie; LI Jia-hui

    2006-01-01

    Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson's trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group,ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.

  5. Master sintering curve: A practical approach to its construction

    Directory of Open Access Journals (Sweden)

    Pouchly V.

    2010-01-01

    Full Text Available The concept of a Master Sintering Curve (MSC is a strong tool for optimizing the sintering process. However, constructing the MSC from sintering data involves complicated and time-consuming calculations. A practical method for the construction of a MSC is presented in the paper. With the help of a few dilatometric sintering experiments the newly developed software calculates the MSC and finds the optimal activation energy of a given material. The software, which also enables sintering prediction, was verified by sintering tetragonal and cubic zirconia, and alumina of two different particle sizes.

  6. Effects of high glucose on mesenchymal stem cell proliferation and differentiation

    DEFF Research Database (Denmark)

    Li, Yu-Ming; Schilling, Tatjana; Benisch, Peggy;

    2007-01-01

    High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized ......High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase...

  7. Kardiovaskulaer regeneration med mesenkymale stamceller

    DEFF Research Database (Denmark)

    Mathiasen, Anders Bruun; Sørensen, Mandana Haack; Jørgensen, Erik;

    2010-01-01

    Treatment with stem cells with a regenerative potential is a new form of therapy that is being studied intensively. Mesenchymal stem cells or stromal cells (MSC) are a promising source of stem cells for regenerative therapy. MSC are easy to isolate and culture, expand in vitro and have...... a multipotent differentiation capacity. Clinical MSC studies on patients with ischaemic heart disease have shown improved left ventricular function and perfusion and also a reduction in infarct size and symptoms. In this short review we provide a status on MSC regenerative treatment in cardiovascular disease....

  8. Enhanced neuro-therapeutic potential of Wharton's Jelly-derived mesenchymal stem cells in comparison with bone marrow mesenchymal stem cells culture.

    Science.gov (United States)

    Drela, Katarzyna; Lech, Wioletta; Figiel-Dabrowska, Anna; Zychowicz, Marzena; Mikula, Michał; Sarnowska, Anna; Domanska-Janik, Krystyna

    2016-04-01

    Substantial inconsistencies in mesenchymal stem (stromal) cell (MSC) therapy reported in early translational and clinical studies may indicate need for selection of the proper cell population for any particular therapeutic purpose. In the present study we have examined stromal stem cells derived either from umbilical cord Wharton's Jelly (WJ-MSC) or bone marrow (BM-MSC) of adult, healthy donors. The cells characterized in accordance with the International Society for Cellular Therapy (ISCT) indications as well as other phenotypic and functional parameters have been compared under strictly controlled culture conditions. WJ-MSC, in comparison with BM-MSC, exhibited a higher proliferation rate, a greater expansion capability being additionally stimulated under low-oxygen atmosphere, enhanced neurotrophic factors gene expression and spontaneous tendency toward a neural lineage differentiation commitment confirmed by protein and gene marker induction. Our data suggest that WJ-MSC may represent an example of immature-type "pre-MSC," where a substantial cellular component is embryonic-like, pluripotent derivatives with the default neural-like differentiation. These cells may contribute in different extents to nearly all classical MSC populations adversely correlated with the age of cell donors. Our data suggest that neuro-epithelial markers, like nestin, stage specific embryonic antigens-4 or α-smooth muscle actin expressions, may serve as useful indicators of MSC culture neuro-regeneration-associated potency. PMID:26971678

  9. Hypoxia Created Human Mesenchymal Stem Cell Sheet for Prevascularized 3D Tissue Construction.

    Science.gov (United States)

    Zhang, Lijun; Xing, Qi; Qian, Zichen; Tahtinen, Mitchell; Zhang, Zhaoqiang; Shearier, Emily; Qi, Shaohai; Zhao, Feng

    2016-02-01

    3D tissue based on human mesenchymal stem cell (hMSC) sheets offers many interesting opportunities for regenerating multiple types of connective tissues. Prevascularizing hMSC sheets with endothelial cells (ECs) will improve 3D tissue performance by supporting cell survival and accelerating integration with host tissue. It is hypothesized that hypoxia cultured hMSC sheets can promote microvessel network formation and preserve stemness of hMSCs. This study investigates the vascularization of hMSC sheets under different oxygen tensions. It is found that the HN condition, in which hMSC sheets formed under physiological hypoxia (2% O2 ) and then cocultured with ECs under normoxia (20% O2 ), enables longer and more branched microvessel network formation. The observation is corroborated by higher levels of angiogenic factors in coculture medium. Additionally, the hypoxic hMSC sheet is more uniform and less defective, which facilitates fabrication of 3D prevascularized tissue construct by layering the prevascularized hMSC sheets and maturing in rotating wall vessel bioreactor. The hMSCs in the 3D construct still maintain multilineage differentiation ability, which indicates the possible application of the 3D construct for various connective tissues regeneration. These results demonstrate that hypoxia created hMSC sheets benefit the microvessel growth and it is feasible to construct 3D prevascularized tissue construct using the prevascularized hMSC sheets.

  10. Cardiac migration of endogenous mesenchymal stromal cells in patients with inflammatory cardiomyopathy.

    Science.gov (United States)

    Schmidt-Lucke, Caroline; Escher, Felicitas; Van Linthout, Sophie; Kühl, Uwe; Miteva, Kapka; Ringe, Jochen; Zobel, Thomas; Schultheiss, Heinz-Peter; Tschöpe, Carsten

    2015-01-01

    Introduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with (n = 23) or without (n = 6) CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45(-)CD34(-)CD11b(-)CD73(+)CD90(+) cells accounted for 0.010 [0.0025-0.048]%/peripheral mononuclear cell (PMNC) and as CD45(-)CD34(-)CD11b(-)CD73(+)CD105(+) cells for 0.019 [0.0026-0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% (P TCG of circulating MSC and numbers of MSC (CD45(-)CD34(-)CD90(+)CD105(+)) in EMB (r = -0.73, P < 0.005). SDF-1α was the strongest predictor for increased MSC in EMB (P < 0.005, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation. PMID:25814787

  11. Intramyocardial delivery of mesenchymal stem cell-seeded hydrogel preserves cardiac function and attenuates ventricular remodeling after myocardial infarction.

    Directory of Open Access Journals (Sweden)

    Eva Mathieu

    Full Text Available BACKGROUND: To improve the efficacy of bone marrow-derived mesenchymal stem cell (MSC therapy targeted to infarcted myocardium, we investigated whether a self-setting silanized hydroxypropyl methylcellulose (Si-HPMC hydrogel seeded with MSC (MSC+hydrogel could preserve cardiac function and attenuate left ventricular (LV remodeling during an 8-week follow-up study in a rat model of myocardial infarction (MI. METHODOLOGY/PRINCIPAL FINDING: Si-HPMC hydrogel alone, MSC alone or MSC+hydrogel were injected into the myocardium immediately after coronary artery ligation in female Lewis rats. Animals in the MSC+hydrogel group showed an increase in cardiac function up to 28 days after MI and a mid-term prevention of cardiac function alteration at day 56. Histological analyses indicated that the injection of MSC+hydrogel induced a decrease in MI size and an increase in scar thickness and ultimately limited the transmural extent of MI. These findings show that intramyocardial injection of MSC+hydrogel induced short-term recovery of ventricular function and mid-term attenuation of remodeling after MI. CONCLUSION/SIGNIFICANCE: These beneficial effects may be related to the specific scaffolding properties of the Si-HPMC hydrogel that may provide the ability to support MSC injection and engraftment within myocardium.

  12. Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential.

    Directory of Open Access Journals (Sweden)

    Darilang Mawrie

    Full Text Available Mesenchymal stem cells (MSC have been proposed as suitable candidates for cell therapy for neurological disorderssince they exhibit good neuronal differentiation capacity. However, for better therapeutic outcomes, it is necessary to isolate MSC from a suitable tissue sourcethat posses high neuronal differentiation. In this context, we isolated MSC from extra ocular muscle (EOM tissue and tested the in vitro neuronal differentiation potential. In the current study, EOM tissue derived MSC were characterized and compared with bone marrow derived MSC. We found that EOM derived MSC proliferated as a monolayer and showed similarities in morphology, growth properties and cell surface marker expression with bone marrow derived MSC and expressed high levels of NES, OCT4, NANOG and SOX2 in its undifferentiated state. They also expressed embryonic cell surface marker SSEA4 and their intracellular mitochondrial distribution pattern was similar to that of multipotent stem cells. Although EOM derived MSC differentiated readily into adipocytes, osteocytes and chondrocytes, they differentiated more efficiently into neuroectodermal cells. The differentiation into neuroectodermal cellswas confirmed by the expression of neuronal markers NGFR and MAP2B. Thus, EOM derived MSC might be good candidates for stem cell based therapies for treating neurodegenerative diseases.

  13. Maintenance of differentiation potential of human bone marrow mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene despite of extensive proliferation

    International Nuclear Information System (INIS)

    Human bone marrow mesenchymal stem cells (hMSC) represent a population of stem cells that are capable of differentiation into multiple lineages. However, these cells exhibit senescence-associated growth arrest and phenotypic changes during long-term in vitro culture. We have recently demonstrated that overexpression of human telomerase reverse transcriptase (hTERT) in hMSC reconstitutes telomerase activity and extends life span of the cells [Nat. Biotechnol. 20 (2002) 592]. In the present study, we have performed extensive characterization of three independent cell lines derived from the parental hMSC-TERT cell line based on different plating densities during expansion in culture: 1:2 (hMSC-TERT2), 1:4 (hMSC-TERT4), and 1:20 (hMSC-TERT20). The 3 cell lines exhibited differences in morphology and growth rates but they all maintained the characteristics of self-renewing stem cells and the ability to differentiate into multiple mesoderm-type cell lineages: osteoblasts, adipocytes, chondrocytes, and endothelial-like cells over a 3-year period in culture. Also, surface marker studies using flow cytometry showed a pattern similar to that known from normal hMSC. Thus, telomerization of hMSC by hTERT overexpression maintains the stem cell phenotype of hMSC and it may be a useful tool for obtaining enough number of cells with a stable phenotype for mechanistic studies of cell differentiation and for tissue engineering protocols

  14. Mesenchymal Stem Cells from Human Extra Ocular Muscle Harbor Neuroectodermal Differentiation Potential

    Science.gov (United States)

    Magdalene, Damaris; Bhattacharyya, Jina; Jaganathan, Bithiah Grace

    2016-01-01

    Mesenchymal stem cells (MSC) have been proposed as suitable candidates for cell therapy for neurological disorderssince they exhibit good neuronal differentiation capacity. However, for better therapeutic outcomes, it is necessary to isolate MSC from a suitable tissue sourcethat posses high neuronal differentiation. In this context, we isolated MSC from extra ocular muscle (EOM) tissue and tested the in vitro neuronal differentiation potential. In the current study, EOM tissue derived MSC were characterized and compared with bone marrow derived MSC. We found that EOM derived MSC proliferated as a monolayer and showed similarities in morphology, growth properties and cell surface marker expression with bone marrow derived MSC and expressed high levels of NES, OCT4, NANOG and SOX2 in its undifferentiated state. They also expressed embryonic cell surface marker SSEA4 and their intracellular mitochondrial distribution pattern was similar to that of multipotent stem cells. Although EOM derived MSC differentiated readily into adipocytes, osteocytes and chondrocytes, they differentiated more efficiently into neuroectodermal cells. The differentiation into neuroectodermal cellswas confirmed by the expression of neuronal markers NGFR and MAP2B. Thus, EOM derived MSC might be good candidates for stem cell based therapies for treating neurodegenerative diseases. PMID:27248788

  15. Optimizing patient derived mesenchymal stem cells as virus carriers for a Phase I clinical trial in ovarian cancer

    Directory of Open Access Journals (Sweden)

    Mader Emily K

    2013-01-01

    Full Text Available Abstract Background Mesenchymal stem cells (MSC can serve as carriers to deliver oncolytic measles virus (MV to ovarian tumors. In preparation for a clinical trial to use MSC as MV carriers, we obtained cells from ovarian cancer patients and evaluated feasibility and safety of this approach. Methods MSC from adipose tissues of healthy donors (hMSC and nine ovarian cancer patients (ovMSC were characterized for susceptibility to virus infection and tumor homing abilities. Results Adipose tissue (range 0.16-3.96 grams from newly diagnosed and recurrent ovarian cancer patients yielded about 7.41×106 cells at passage 1 (range 4–9 days. Phenotype and doubling times of MSC were similar between ovarian patients and healthy controls. The time to harvest of 3.0×108 cells (clinical dose could be achieved by day 14 (range, 9–17 days. Two of nine samples tested had an abnormal karyotype represented by trisomy 20. Despite receiving up to 1.6×109 MSC/kg, no tumors were seen in SCID beige mice and MSC did not promote the growth of SKOV3 human ovarian cancer cells in mice. The ovMSC migrated towards primary ovarian cancer samples in chemotaxis assays and to ovarian tumors in athymic mice. Using non-invasive SPECT-CT imaging, we saw rapid co-localization, within 5–8 minutes of intraperitoneal administration of MV infected MSC to the ovarian tumors. Importantly, MSC can be pre-infected with MV, stored in liquid nitrogen and thawed on the day of infusion into mice without loss of activity. MV infected MSC, but not virus alone, significantly prolonged the survival of measles immune ovarian cancer bearing animals. Conclusions These studies confirmed the feasibility of using patient derived MSC as carriers for oncolytic MV therapy. We propose an approach where MSC from ovarian cancer patients will be expanded, frozen and validated to ensure compliance with the release criteria. On the treatment day, the cells will be thawed, washed, mixed with virus, briefly

  16. A molecular classification of human mesenchymal stromal cells.

    Science.gov (United States)

    Rohart, Florian; Mason, Elizabeth A; Matigian, Nicholas; Mosbergen, Rowland; Korn, Othmar; Chen, Tyrone; Butcher, Suzanne; Patel, Jatin; Atkinson, Kerry; Khosrotehrani, Kiarash; Fisk, Nicholas M; Lê Cao, Kim-Anh; Wells, Christine A

    2016-01-01

    Mesenchymal stromal cells (MSC) are widely used for the study of mesenchymal tissue repair, and increasingly adopted for cell therapy, despite the lack of consensus on the identity of these cells. In part this is due to the lack of specificity of MSC markers. Distinguishing MSC from other stromal cells such as fibroblasts is particularly difficult using standard analysis of surface proteins, and there is an urgent need for improved classification approaches. Transcriptome profiling is commonly used to describe and compare different cell types; however, efforts to identify specific markers of rare cellular subsets may be confounded by the small sample sizes of most studies. Consequently, it is difficult to derive reproducible, and therefore useful markers. We addressed the question of MSC classification with a large integrative analysis of many public MSC datasets. We derived a sparse classifier (The Rohart MSC test) that accurately distinguished MSC from non-MSC samples with >97% accuracy on an internal training set of 635 samples from 41 studies derived on 10 different microarray platforms. The classifier was validated on an external test set of 1,291 samples from 65 studies derived on 15 different platforms, with >95% accuracy. The genes that contribute to the MSC classifier formed a protein-interaction network that included known MSC markers. Further evidence of the relevance of this new MSC panel came from the high number of Mendelian disorders associated with mutations in more than 65% of the network. These result in mesenchymal defects, particularly impacting on skeletal growth and function. The Rohart MSC test is a simple in silico test that accurately discriminates MSC from fibroblasts, other adult stem/progenitor cell types or differentiated stromal cells. It has been implemented in the www.stemformatics.org resource, to assist researchers wishing to benchmark their own MSC datasets or data from the public domain. The code is available from the CRAN

  17. Articular cartilage-derived cells hold a strong osteogenic differentiation potential in comparison to mesenchymal stem cells in vitro

    International Nuclear Information System (INIS)

    Cartilaginous matrix-degenerative diseases like osteoarthritis (OA) are characterized by gradual cartilage erosion, and also by increased presence of cells with mesenchymal stem cell (MSC) character within the affected tissues. Moreover, primary chondrocytes long since are known to de-differentiate in vitro and to be chondrogenically re-differentiable. Since both findings appear to conflict with each other, we quantitatively assessed the mesenchymal differentiation potential of OA patient cartilage-derived cells (CDC) towards the osteogenic and adipogenic lineage in vitro and compared it to that of MSC isolated from adipose tissue (adMSC) of healthy donors. We analyzed expression of MSC markers CD29, CD44, CD105, and CD166, and, following osteogenic and adipogenic induction in vitro, quantified their expression of osteogenic and adipogenic differentiation markers. Furthermore, CDC phenotype and proliferation were monitored. We found that CDC exhibit an MSC CD marker expression pattern similar to adMSC and a similar increase in proliferation rate during osteogenic differentiation. In contrast, the marked reduction of proliferation observed during adipogenic differentiation of adMSC was absent in CDC. Quantification of differentiation markers revealed a strong osteogenic differentiation potential for CDC, however almost no capacity for adipogenic differentiation. Since in the pathogenesis of OA, cartilage degeneration coincides with high bone turnover rates, the high osteogenic differentiation potential of OA patient-derived CDC may affect clinical therapeutic regimens aiming at autologous cartilage regeneration in these patients. - Highlights: • We analyze the mesenchymal differentiation capacity of cartilage-derived cells (CDC). • CDC express mesenchymal stem cell (MSC) markers CD29, CD44, CD105, and CD166. • CDC and MSC proliferation is reduced in adipogenesis and increased in osteogenesis. • Adipogenic differentiation is virtually absent in CDC, but

  18. Articular cartilage-derived cells hold a strong osteogenic differentiation potential in comparison to mesenchymal stem cells in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Salamon, Achim, E-mail: achim.salamon@med.uni-rostock.de [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany); Jonitz-Heincke, Anika, E-mail: anika.jonitz@med.uni-rostock.de [Biomechanics and Implant Technology Research Laboratory, Department of Orthopedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock (Germany); Adam, Stefanie, E-mail: stefanie.adam@med.uni-rostock.de [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany); Rychly, Joachim, E-mail: joachim.rychly@med.uni-rostock.de [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany); Müller-Hilke, Brigitte, E-mail: brigitte.mueller-hilke@med.uni-rostock.de [Institute of Immunology, Rostock University Medical Center, Schillingallee 68, D-18057 Rostock (Germany); Bader, Rainer, E-mail: rainer.bader@med.uni-rostock.de [Biomechanics and Implant Technology Research Laboratory, Department of Orthopedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock (Germany); Lochner, Katrin, E-mail: katrin.lochner@med.uni-rostock.de [Biomechanics and Implant Technology Research Laboratory, Department of Orthopedics, Rostock University Medical Center, Doberaner Straße 142, D-18057 Rostock (Germany); Peters, Kirsten, E-mail: kirsten.peters@med.uni-rostock.de [Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, D-18057 Rostock (Germany)

    2013-11-01

    Cartilaginous matrix-degenerative diseases like osteoarthritis (OA) are characterized by gradual cartilage erosion, and also by increased presence of cells with mesenchymal stem cell (MSC) character within the affected tissues. Moreover, primary chondrocytes long since are known to de-differentiate in vitro and to be chondrogenically re-differentiable. Since both findings appear to conflict with each other, we quantitatively assessed the mesenchymal differentiation potential of OA patient cartilage-derived cells (CDC) towards the osteogenic and adipogenic lineage in vitro and compared it to that of MSC isolated from adipose tissue (adMSC) of healthy donors. We analyzed expression of MSC markers CD29, CD44, CD105, and CD166, and, following osteogenic and adipogenic induction in vitro, quantified their expression of osteogenic and adipogenic differentiation markers. Furthermore, CDC phenotype and proliferation were monitored. We found that CDC exhibit an MSC CD marker expression pattern similar to adMSC and a similar increase in proliferation rate during osteogenic differentiation. In contrast, the marked reduction of proliferation observed during adipogenic differentiation of adMSC was absent in CDC. Quantification of differentiation markers revealed a strong osteogenic differentiation potential for CDC, however almost no capacity for adipogenic differentiation. Since in the pathogenesis of OA, cartilage degeneration coincides with high bone turnover rates, the high osteogenic differentiation potential of OA patient-derived CDC may affect clinical therapeutic regimens aiming at autologous cartilage regeneration in these patients. - Highlights: • We analyze the mesenchymal differentiation capacity of cartilage-derived cells (CDC). • CDC express mesenchymal stem cell (MSC) markers CD29, CD44, CD105, and CD166. • CDC and MSC proliferation is reduced in adipogenesis and increased in osteogenesis. • Adipogenic differentiation is virtually absent in CDC, but

  19. Interleukin-17A-Induced Human Mesenchymal Stem Cells Are Superior Modulators of Immunological Function.

    Science.gov (United States)

    Sivanathan, Kisha Nandini; Rojas-Canales, Darling M; Hope, Christopher M; Krishnan, Ravi; Carroll, Robert P; Gronthos, Stan; Grey, Shane T; Coates, Patrick T

    2015-09-01

    Interferon-γ (IFN-γ)-preactivated mesenchymal stem cells (MSC-γ) are highly immunosuppressive but immunogenic in vivo due to their inherent expression of major histocompatibility (MHC) molecules. Here, we present an improved approach where we modified human bone marrow-derived MSC with interleukin-17A (MSC-17) to enhance T cell immunosuppression but not their immunogenicity. MSC-17, unlike MSC-γ, showed no induction or upregulation of MHC class I, MHC class II, and T cell costimulatory molecule CD40, but maintained normal MSC morphology and phenotypic marker expression. When cocultured with phytohemagglutinin (PHA)-activated human T cells, MSCs-17 were potent suppressors of T cell proliferation. Furthermore, MSC-17 inhibited surface CD25 expression and suppressed the elaboration of Th1 cytokines, IFN-γ, tumor necrosis factor-α (TNF-α), and IL-2 when compared with untreated MSCs (UT-MSCs). T cell suppression by MSC-17 correlated with increased IL-6 but not with indoleamine 2,3-dioxygenase 1, cyclooxygenase 1, and transforming growth factor β-1. MSC-17 but not MSC-γ consistently induced CD4(+) CD25(high) CD127(low) FoxP3(+) regulatory T cells (iTregs) from PHA-activated CD4(+) CD25(-) T cells. MSC-induced iTregs expressed CD39, CD73, CD69, OX40, cytotoxic T-lymphocyte associated antigen-4 (CTLA-4), and glucocorticoid-induced TNFR-related protein (GITR). These suppressive MSCs-17 can engender Tregs to potently suppress T cell activation with minimal immunogenicity and thus represent a superior T cell immunomodulator for clinical application. PMID:26037953

  20. Mesenchymal stem cells directly interact with breast cancer cells and promote tumor cell growth in vitro and in vivo.

    Science.gov (United States)

    Mandel, Katharina; Yang, Yuanyuan; Schambach, Axel; Glage, Silke; Otte, Anna; Hass, Ralf

    2013-12-01

    Cellular interactions were investigated between human mesenchymal stem cells (MSC) and human breast cancer cells. Co-culture of the two cell populations was associated with an MSC-mediated growth stimulation of MDA-MB-231 breast cancer cells. A continuous expansion of tumor cell colonies was progressively surrounded by MSC(GFP) displaying elongated cell bodies. Moreover, some MSC(GFP) and MDA-MB-231(cherry) cells spontaneously generated hybrid/chimeric cell populations, demonstrating a dual (green fluorescent protein+cherry) fluorescence. During a co-culture of 5-6 days, MSC also induced expression of the GPI-anchored CD90 molecule in breast cancer cells, which could not be observed in a transwell assay, suggesting the requirement of direct cellular interactions. Indeed, MSC-mediated CD90 induction in the breast cancer cells could be partially blocked by a gap junction inhibitor and by inhibition of the notch signaling pathway, respectively. Similar findings were observed in vivo by which a subcutaneous injection of a co-culture of primary MSC with MDA-MB-231(GFP) cells into NOD/scid mice exhibited an about 10-fold increased tumor size and enhanced metastatic capacity as compared with the MDA-MB-231(GFP) mono-culture. Flow cytometric evaluation of the co-culture tumors revealed more than 90% of breast cancer cells with about 3% of CD90-positive cells, also suggesting an MSC-mediated in vivo induction of CD90 in MDA-MB-231 cells. Furthermore, immunohistochemical analysis demonstrated an elevated neovascularization and viability in the MSC/MDA-MB-231(GFP)-derived tumors. Together, these data suggested an MSC-mediated growth stimulation of breast cancer cells in vitro and in vivo by which the altered MSC morphology and the appearance of hybrid/chimeric cells and breast cancer-expressing CD90(+) cells indicate mutual cellular alterations.

  1. Multimodality Molecular Imaging of Cardiac Cell Transplantation: Part I. Reporter Gene Design, Characterization, and Optical in Vivo Imaging of Bone Marrow Stromal Cells after Myocardial Infarction.

    Science.gov (United States)

    Parashurama, Natesh; Ahn, Byeong-Cheol; Ziv, Keren; Ito, Ken; Paulmurugan, Ramasamy; Willmann, Jürgen K; Chung, Jaehoon; Ikeno, Fumiaki; Swanson, Julia C; Merk, Denis R; Lyons, Jennifer K; Yerushalmi, David; Teramoto, Tomohiko; Kosuge, Hisanori; Dao, Catherine N; Ray, Pritha; Patel, Manishkumar; Chang, Ya-Fang; Mahmoudi, Morteza; Cohen, Jeff Eric; Goldstone, Andrew Brooks; Habte, Frezghi; Bhaumik, Srabani; Yaghoubi, Shahriar; Robbins, Robert C; Dash, Rajesh; Yang, Phillip C; Brinton, Todd J; Yock, Paul G; McConnell, Michael V; Gambhir, Sanjiv S

    2016-09-01

    Purpose To use multimodality reporter-gene imaging to assess the serial survival of marrow stromal cells (MSC) after therapy for myocardial infarction (MI) and to determine if the requisite preclinical imaging end point was met prior to a follow-up large-animal MSC imaging study. Materials and Methods Animal studies were approved by the Institutional Administrative Panel on Laboratory Animal Care. Mice (n = 19) that had experienced MI were injected with bone marrow-derived MSC that expressed a multimodality triple fusion (TF) reporter gene. The TF reporter gene (fluc2-egfp-sr39ttk) consisted of a human promoter, ubiquitin, driving firefly luciferase 2 (fluc2), enhanced green fluorescent protein (egfp), and the sr39tk positron emission tomography reporter gene. Serial bioluminescence imaging of MSC-TF and ex vivo luciferase assays were performed. Correlations were analyzed with the Pearson product-moment correlation, and serial imaging results were analyzed with a mixed-effects regression model. Results Analysis of the MSC-TF after cardiac cell therapy showed significantly lower signal on days 8 and 14 than on day 2 (P = .011 and P = .001, respectively). MSC-TF with MI demonstrated significantly higher signal than MSC-TF without MI at days 4, 8, and 14 (P = .016). Ex vivo luciferase activity assay confirmed the presence of MSC-TF on days 8 and 14 after MI. Conclusion Multimodality reporter-gene imaging was successfully used to assess serial MSC survival after therapy for MI, and it was determined that the requisite preclinical imaging end point, 14 days of MSC survival, was met prior to a follow-up large-animal MSC study. (©) RSNA, 2016 Online supplemental material is available for this article. PMID:27308957

  2. Systemic mesenchymal stem cell administration enhances bone formation in fracture repair but not load-induced bone formation

    Directory of Open Access Journals (Sweden)

    AE Rapp

    2015-01-01

    Full Text Available Mesenchymal stem cells (MSC were shown to support bone regeneration, when they were locally transplanted into poorly healing fractures. The benefit of systemic MSC transplantation is currently less evident. There is consensus that systemically applied MSC are recruited to the site of injury, but it is debated whether they actually support bone formation. Furthermore, the question arises as to whether circulating MSC are recruited only in case of injury or whether they also participate in mechanically induced bone formation. To answer these questions we injected green fluorescent protein (GFP-labelled MSC into C57BL/6J mice, which were subjected either to a femur osteotomy or to non-invasive mechanical ulna loading to induce bone formation. We detected GFP-labelled MSC in the early (day 10 and late fracture callus (day 21 by immunohistochemistry. Stromal cell-derived factor 1 (SDF-1 or CXCL-12, a key chemokine for stem cell attraction, was strongly expressed by virtually all cells near the osteotomy – indicating that SDF-1 may mediate cell migration to the site of injury. We found no differences in SDF-1 expression between the groups. Micro-computed tomography (µCT revealed significantly more bone in the callus of the MSC treated mice compared to untreated controls. The bending stiffness of callus was not significantly altered after MSC-application. In contrast, we failed to detect GFP-labelled MSC in the ulna after non-invasive mechanical loading. Histomorphometry and µCT revealed a significant load-induced increase in bone formation; however, no further increase was found after MSC administration. Concluding, our results suggest that systemically administered MSC are recruited and support bone formation only in case of injury but not in mechanically induced bone formation.

  3. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.

    Directory of Open Access Journals (Sweden)

    Massimo Giuliani

    Full Text Available Human bone marrow mesenchymal stem cells (BM-MSC are multipotent progenitor cells that have transient immunomodulatory properties on Natural Killer (NK cells, Dendritic Cells (DC, and T cells. This study compared the use of MSC isolated from bone marrow and fetal liver (FL-MSC to determine which displayed the most efficient immunosuppressive effects on T cell activation. Although both types of MSC exhibit similar phenotype profile, FL-MSC displays a much more extended in vitro life-span and immunomodulatory properties. When co-cultured with CD3/CD28-stimulated T cells, both BM-MSC and FL-MSC affected T cell proliferation by inhibiting their entry into the cell cycle, by inducing the down-regulation of phospho-retinoblastoma (pRb, cyclins A and D1, as well as up-regulating p27(kip1 expression. The T cell inhibition by MSC was not due to the soluble HLA-G5 isoform, but to the surface expression of HLA-G1, as shown by the need of cell-cell contact and by the use of neutralizing anti-HLA-G antibodies. To note, in a HLA-G-mediated fashion, MSC facilitated the expansion of a CD4(low/CD8(low T subset that had decreased secretion of IFN-γ, and an induced secretion of the immunomodulatory cytokine IL-10. Because of their longer lasting in vitro immunosuppressive properties, mainly mediated by HLA-G, and their more efficient induction of IL-10 production and T cell apoptosis, fetal liver MSC could be considered a new tool for MSC therapy to prevent allograft rejection.

  4. Spectra and vegetation index variations in moss soil crust in different seasons, and in wet and dry conditions

    Science.gov (United States)

    Fang, Shibo; Yu, Weiguo; Qi, Yue

    2015-06-01

    Similar to vascular plants, non-vascular plant mosses have different periods of seasonal growth. There has been little research on the spectral variations of moss soil crust (MSC) over different growth periods. Few studies have paid attention to the difference in spectral characteristics between wet MSC that is photosynthesizing and dry MSC in suspended metabolism. The dissimilarity of MSC spectra in wet and dry conditions during different seasons needs further investigation. In this study, the spectral reflectance of wet MSC, dry MSC and the dominant vascular plant (Artemisia) were characterized in situ during the summer (July) and autumn (September). The variations in the normalized difference vegetation index (NDVI), biological soil crust index (BSCI) and CI (crust index) in different seasons and under different soil moisture conditions were also analyzed. It was found that (1) the spectral characteristics of both wet and dry MSCs varied seasonally; (2) the spectral features of wet MSC appear similar to those of the vascular plant, Artemisia, whether in summer or autumn; (3) both in summer and in autumn, much higher NDVI values were acquired for wet than for dry MSC (0.6 ∼ 0.7 vs. 0.3 ∼ 0.4 units), which may lead to misinterpretation of vegetation dynamics in the presence of MSC and with the variations in rainfall occurring in arid and semi-arid zones; and (4) the BSCI and CI values of wet MSC were close to that of Artemisia in both summer and autumn, indicating that BSCI and CI could barely differentiate between the wet MSC and Artemisia.

  5. Effect of human umbilical cord mesenchymal stem cell-secretion on proliferation and apoptosis in hepatocytes%人脐带间充质干细胞分泌物对肝细胞增殖和凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    黎娇; 朱争艳; 杜智; 骆莹; 王鹏; 高英堂

    2010-01-01

    目的 探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUC MSC)旁分泌物质在体外对肝细胞再生和凋亡的影响.方法 利用Ⅳ型胶原酶和胰酶消化法从脐带中分离间充质干细胞,制备含有HUCMSC旁分泌物质的条件培养基(mesenchymal stem cells-conditioned medium,MSC-CM),采用低浓度胶原酶原位循环灌流法分离肝细胞.试验分为对照组、2%MSC-CM组和8%MSC-CM组三组.采用MTT比色法观察不同浓度MSC-CM对正常肝细胞增殖的影响.测定上清中尿素、白蛋白的含量,观察不同浓度MSC-CM对肝细胞功能的影响.利用放线菌素D和肿瘤坏死因子α诱导肝细胞凋亡,采用细胞活性分析试剂盒检测不同浓度MSC-CM对肝细胞凋亡的影响.结果 与对照组比较,2%MSC-CM组吸光度(A)540nm值(P<0.01)以及上清尿素和白蛋白含量显著升高(P<0.01),肝细胞存活率增加(P<0.05);8%MSC-CM组与对照组无显著差异.结论 低浓度的MSC-CM在体外可以刺激正常肝细胞再生,抑制受损肝细胞凋亡,改善肝细胞功能.%Objective To investigate the effect of human umbilical cord mesenchymal stem cell paracrine substance on proliferation and apoptosis of liver cells in vitro. Methods Mesenchymal stem cells (MSC)were separated from human umbilical cord with type Ⅳ collagenase and trypsogen digestion method and cultured in vitro. The human umbilical cord mesenchymal stem cells-conditioned medium(MSC-CM) which contain paracrine substance of human umbilical cord mesenchymal stem cells (HUCMSC) was prepared. Hepatocytes were isolated from SD rats by low concentration collagenase perfusion procedure. There were three groups in the experiment, control group, 2% MSC-CM group and 8% MSC-CM group. The proliferation of normal hepatocytes were assayed with MTT method. We detected the urea and albumin level in culture supernatant to assay the hepatocyte function under different concentration MSC-CM. Hepatocytes were

  6. Comparison of Modeling Methods of Fresh Jujube Soluble Solids Measurement by NIR Spectroscopy%鲜枣可溶性固形物可见/近红外光谱检测建模方法比较

    Institute of Scientific and Technical Information of China (English)

    张淑娟; 张海红; 赵艳茹; 赵华民

    2012-01-01

    对采摘于一枣园的180个壶瓶枣样本,随机分成150个样本校正集和30个样本预测集.用FieldSpec3光谱仪采集光谱,并进行多元散射校正(MSC)预处理,之后分别利用连续投影算法(SPA)和逐步回归法( SRA)提取特征波长,并结合光谱理论分析确定,再分别基于偏最小二乘法(PLS)和最小二乘-支持向量机(LS-SVM)建立壶瓶枣可溶性固形物含量预测的简化模型和全波段模型.结果表明,全波段PLS模型预测的相关系数和预测均方根误差分别为0.887 4和1.088 9,预测效果最好;建立的MSC - SPA - PLS模型预测的相关系数和均方根误差分别为0.799 0和1.407 8,建立的MSC - SRA - PLS模型预测的相关系数和均方根误差分别为0.822 4和1.3851,与全波段的MSC - PLS相比,精度均降低;建立的MSC - SPA - LS - SVM模型预测的相关系数和均方根误差分别为0.796 3和1.145 8,与全波段的MSC - LS - SVM相比,精度提高;建立的MSC - SRA - LS - SVM模型预测精度很低,不适用.%Totally 180 samples coining from one orchard were divided into calibration set with 150 samples and prediction set with 30 samples. Field Spec3 spectrometer was used for collecting spectra data of 180 fresh jujube samples separately. Then successive projection algorithm and stepwise regression analysis combined with spectral theory were used to process the spectral data after MSC pretreatment. Characteristic wavelengths of 150 samples in calibration set were selected by using SPA and SRA, and the partial least square (PLS) and LS - SVM methods were used to establish models of the fresh jujube soluble solids with the whole spectrum and characteristic wavelengths selected by using SPA and SRA. At last, the models of MSC - PLS model, MSC - LS - SVM model, the MSC - SPA - PLS model, the MSC -SPA - LS - SVM model, the MSC - SRA - PLS model and the MSC - SRA - LS - SVM model were used to predict the soluble solids of 30 samples in the prediction set. The

  7. Evaluation of intrarenal mesenchymal stem cell injection for treatment of chronic kidney disease in cats: a pilot study.

    Science.gov (United States)

    Quimby, Jessica M; Webb, Tracy L; Gibbons, Debra S; Dow, Steven W

    2011-06-01

    The feasibility of autologous intrarenal mesenchymal stem cell (MSC) therapy in cats with chronic kidney disease (CKD) was investigated. Six cats (two healthy, four with CKD) received a single unilateral intrarenal injection of autologous bone marrow-derived or adipose tissue-derived MSC (bmMSC or aMSC) via ultrasound guidance. Minimum database and glomerular filtration rate (GFR) via nuclear scintigraphy were determined pre-injection, at 7 days and at 30 days post-injection. Intrarenal injection did not induce immediate or long-term adverse effects. Two cats with CKD that received aMSC experienced modest improvement in GFR and a mild decrease in serum creatinine concentration. Despite the possible benefits of intrarenal MSC injections for CKD cats, the number of sedations and interventions required to implement this approach would likely preclude widespread clinical application. We concluded that MSC could be transferred safely by ultrasound-guided intrarenal injection in cats, but that alternative sources and routes of MSC therapy should be investigated. PMID:21334237

  8. Aging of marrow stromal (skeletal) stem cells and their contribution to age-related bone loss

    DEFF Research Database (Denmark)

    Bellantuono, Ilaria; Aldahmash, Abdullah; Kassem, Moustapha

    2009-01-01

    Marrow stromal cells (MSC) are thought to be stem cells with osteogenic potential and therefore responsible for the repair and maintenance of the skeleton. Age related bone loss is one of the most prevalent diseases in the elder population. It is controversial whether MSC undergo a process of aging...

  9. Development of the multistep compound process calculation code

    Energy Technology Data Exchange (ETDEWEB)

    Kawano, Toshihiko [Kyushu Univ., Fukuoka (Japan)

    1998-03-01

    A program `cmc` has been developed to calculate the multistep compound (MSC) process by Feshback-Kerman-Koonin. A radial overlap integral in the transition matrix element is calculated microscopically, and comparisons are made for neutron induced {sup 93}Nb reactions. Strengths of the two-body interaction V{sub 0} are estimated from the total MSC cross sections. (author)

  10. Protocols for in vitro Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adipogenic Lineages.

    Science.gov (United States)

    Ciuffreda, Maria Chiara; Malpasso, Giuseppe; Musarò, Paola; Turco, Valentina; Gnecchi, Massimiliano

    2016-01-01

    Mesenchymal stem cells (MSC) possess high plasticity and the potential to differentiate into several different cell types; this characteristic has implications for cell therapy and reparative biotechnologies. MSC have been originally isolated from the bone marrow (BM-MSC), but they have been found also in other tissues such as adipose tissue, cord blood, synovium, skeletal muscle, and lung. MSC are able to differentiate in vitro and in vivo into several cell types such as bone, osteocytes, chondrocytes, adipocytes, and skeletal myocytes, just to name a few.During the last two decades, an increasing number of studies have proven the therapeutic potential of MSC for the treatment of neurodegenerative diseases, spinal cord and brain injuries, cardiovascular diseases, diabetes mellitus, and diseases of the skeleton. Their immuno-privileged profile allows both autologous and allogeneic use. For all these reasons, the scientific appeal of MSC is constantly on the rise.The identity of MSC is currently based on three main criteria: plastic-adherence capacity, defined epitope profile, and capacity to differentiate in vitro into osteocytes, chondrocytes, and adipocytes. Here, we describe standard protocols for the differentiation of BM-MSC into the osteogenic, chondrogenic, and adipogenic lineages. PMID:27236670

  11. Dynamic substructuring for shock spectrum analysis using component mode synthesis

    Science.gov (United States)

    Mcpheeters, Barton W.; Lev, Avivi; Bogert, Philip B.; Scavuzzo, Rudolph J.

    1988-01-01

    Component mode synthesis was used to analyze different types of structures with MSC NASTRAN. The theory and technique of using Multipoint Constraint Equations (MPCs) to connect substructures to each other or to a common foundation is presented. Computation of the dynamic response of the system from shack spectrum inputs was automated using the DMAP programming language of the MSC NASTRAN finite element code.

  12. The effect of allogenic versus autologue mesenchymal stem cells in bone reconstructio

    DEFF Research Database (Denmark)

    Jensen, Stefan; Overgaard, Søren; Ding, Ming

    2008-01-01

    with allogenic MSC (group#3) proved to have a significant higher mean SFE (Fisher's LSD-test). The other groups (#1 and #2) had a slightly higher mean SFE (Table 2). Discussion and Conclusion: There are shown two interesting things in this minor pilot-study. There is a trend showing, that the use of MSC has...

  13. Mesenchymal stem cell therapy in proteoglycan induced arthritis

    NARCIS (Netherlands)

    Swart, J. F.; de Roock, S.; Hofhuis, F. M.; Rozemuller, H.; van den Broek, T.; Moerer, P.; Broere, F.; van Wijk, F.; Kuis, W.; Prakken, B. J.; Martens, a.c.m; Wulffraat, N. M.

    2015-01-01

    Objectives: To explore the immunosuppressive effect and mechanism of action of intraperitoneal (ip) and intra-articular (ia) mesenchymal stem cell (MSC) injection in proteoglycan induced arthritis (PGIA). Methods: MSC were administered ip or ia after establishment of arthritis. We used serial biolum

  14. Multi-Composite Bioactive Osteogenic Sponges Featuring Mesenchymal Stem Cells, Platelet-Rich Plasma, Nanoporous Silicon Enclosures, and Peptide Amphiphiles for Rapid Bone Regeneration

    Directory of Open Access Journals (Sweden)

    Dongmei Fan

    2011-06-01

    Full Text Available A novel bioactive sponge was created with a composite of type I collagen sponges or porous poly(e-caprolactone (PCL scaffolds, platelet-rich plasma (PRP, BMP2-loaded nanoporous silicon enclosure (NSE microparticles, mineralizing peptide amphiphiles (PA, and mesenchymal stem cells (MSC. Primary MSC from cortical bone (CB  tissue proved to form more and larger colony units, as well as produce more mineral matrix under osteogenic differentiation, than MSC from bone marrow (BM. Coating pre-treatments were optimized for maximum cell adhesion and mineralization, while a PRP-based gel carrier was created to efficiently deliver and retain MSC and  microparticles within a porous scaffold while simultaneously promoting cell recruitment, proliferation, and angiogenesis. Components and composite sponges were evaluated for osteogenic differentiation in vitro. Osteogenic sponges were loaded with MSC, PRP, PA, and NSE and implanted subcutaneously in rats to evaluate the formation of bone tissue and angiogenesis in vivo. It was found that the combination of a collagen sponge with CB MSC, PRP, PA, and the BMP2-releasing NSE formed the most bone and was most vascularized by four weeks compared to analogous composites featuring BM MSC or PCL or lacking PRP, PA, and NSE. This study indicates that CB MSC should be considered as an alternative to marrow as a source of stem cells, while the PRP-PA cell and microparticle delivery system may be utilized for diverse tissue engineering applications.

  15. Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation

    Directory of Open Access Journals (Sweden)

    C. M. Raynaud

    2012-01-01

    Full Text Available Mesenchymal stem cells (MSCs are the most promising seed cells for cell therapy and can be isolated from various sources of human adult tissues such as bone marrow (BM-MSC and adipose tissue. However, cells from these tissues must be obtained through invasive procedures. We, therefore, characterized MSCs isolated from fresh placenta (Pl-MSC and fetal membrane (Mb-MSC through morphological and fluorescent-activated cell sorting (FACS. MSC frequency is higher in membrane than placenta (2.14%  ± 0.65 versus 15.67%  ± 0.29%. Pl/Mb-MSCs in vitro expansion potential was significantly higher than BM-MSCs. We demonstrated that one of the MSC-specific marker is sufficient for MSC isolation and that culture in specific media is the optimal way for selecting very homogenous MSC population. These MSCs could be differentiated into mesodermal cells expressing cell markers and cytologic staining consistent with mature osteoblasts and adipocytes. Transcriptomic analysis and cytokine arrays demonstrated broad similarity between placenta- and membrane-derived MSCs and only discrete differences with BM-MSCs with enrichment of networks involved in bone differentiation. Pl/Mb-MSCs displayed higher osteogenic differentiation potential than BM-MSC when their response to osteoactivin was evaluated. Fetal-tissue-derived mesenchymal cells may, therefore, be considered as a major source of MSCs to reach clinical scale banking in particular for bone regeneration.

  16. Exosomes derived from human mesenchymal stem cells confer drug resistance in gastric cancer.

    Science.gov (United States)

    Ji, Runbi; Zhang, Bin; Zhang, Xu; Xue, Jianguo; Yuan, Xiao; Yan, Yongmin; Wang, Mei; Zhu, Wei; Qian, Hui; Xu, Wenrong

    2015-08-01

    Mesenchymal stem cells (MSCs) play an important role in chemoresistance. Exosomes have been reported to modify cellular phenotype and function by mediating cell-cell communication. In this study, we aimed to investigate whether exosomes derived from MSCs (MSC-exosomes) are involved in mediating the resistance to chemotherapy in gastric cancer and to explore the underlying molecular mechanism. We found that MSC-exosomes significantly induced the resistance of gastric cancer cells to 5-fluorouracil both in vivo and ex vivo. MSC-exosomes antagonized 5-fluorouracil-induced apoptosis and enhanced the expression of multi-drug resistance associated proteins, including MDR, MRP and LRP. Mechanistically, MSC-exosomes triggered the activation of calcium/calmodulin-dependent protein kinases (CaM-Ks) and Raf/MEK/ERK kinase cascade in gastric cancer cells. Blocking the CaM-Ks/Raf/MEK/ERK pathway inhibited the promoting role of MSC-exosomes in chemoresistance. Collectively, MSC-exosomes could induce drug resistance in gastric cancer cells by activating CaM-Ks/Raf/MEK/ERK pathway. Our findings suggest that MSC-exosomes have profound effects on modifying gastric cancer cells in the development of drug resistance. Targeting the interaction between MSC-exosomes and cancer cells may help improve the efficacy of chemotherapy in gastric cancer.

  17. Human mesenchymal stem cells: from basic biology to clinical applications

    DEFF Research Database (Denmark)

    Abdallah, B M; Kassem, M

    2008-01-01

    Mesenchymal stem cells (MSC) are a group of clonogenic cells present among the bone marrow stroma and capable of multilineage differentiation into mesoderm-type cells such as osteoblasts, adipocytes and chondrocytes. Due to their ease of isolation and their differentiation potential, MSC are bein...

  18. Adult Stromal (Skeletal, Mesenchymal) Stem Cells: Advances Towards Clinical Applications

    DEFF Research Database (Denmark)

    Kermani, Abbas Jafari; Harkness, Linda; Zaher, Walid;

    2014-01-01

    Mesenchymal Stem Cells (MSC) are non-hematopoietic adult stromal cells that reside in a perivascular niche in close association with pericytes and endothelial cells and possess self-renewal and multi-lineage differentiation capacity. The origin, unique properties, and therapeutic benefits of MSC ...

  19. 77 FR 2076 - National Institute of Mental Health Notice of Closed Meetings

    Science.gov (United States)

    2012-01-13

    ... Neuroscience Center, 6001 Executive Blvd., Room 6153, MSC 9608, Bethesda, MD 20892-9608, (301) 402-8152...: To review and evaluate grant applications. Place: National Institutes of Health Neuroscience Center... Health, NIH Neuroscience Center, 6001 Executive Blvd., Room 6148, MSC 9609, Rockville, MD...

  20. 77 FR 14534 - National Institute of Neurological Disorders and Stroke; Notice of Closed Meetings

    Science.gov (United States)

    2012-03-12

    ... Emphasis Panel, NIH Blueprint for Neuroscience. Date: March 28, 2012. Time: 8 a.m. to 6 p.m. Agenda: To..., Division of Extramural Research, NINDS/NIH/DHHS/Neuroscience Center, 6001 Executive Blvd., Suite 3208, MSC..., NINDS/NIH/DHHS/Neuroscience Center, 6001 Executive Blvd., Suite 3208, MSC 9529, Bethesda, MD 20892,...

  1. Malaysia's Multimedia Super Corridor and Roles of Information Professionals.

    Science.gov (United States)

    Reid, Edna

    In Malaysia, the government is supporting the diffusion of the Internet and is spearheading a project to bring Malaysia into the information age, by helping to design a smart city called the Multimedia Super Corridor (MSC). The MSC is being planned as a high-technology center where world-class multimedia companies can develop state-of-the-art…

  2. 76 FR 13195 - Center for Scientific Review; Notice of Closed Meetings

    Science.gov (United States)

    2011-03-10

    ... of Health, 6701 Rockledge Drive, Room 5220, MSC 7852, Bethesda, MD 20892, 301-443- 5779, prasads@csr... 20892, (301) 435-2211, klosekm@csr.nih.gov . ] Name of Committee: Center for Scientific Review Special..., Room 4184, MSC 7850, Bethesda, MD 20892, (301) 435- 1178, fujiij@csr.nih.gov . Name of...

  3. Tissue distribution and engraftment of human mesenchymal stem cells immortalized by human telomerase reverse transcriptase gene

    DEFF Research Database (Denmark)

    Bentzon, J F; Stenderup, K; Hansen, F D;

    2005-01-01

    Engraftment of mesenchymal stem cells (MSC) in peripheral tissues for replenishing of local stem cell function has been proposed as a therapeutic approach to degenerative diseases. We have previously reported the development of an immortalized human telomerase reverse transcriptase transduced MSC...

  4. 76 FR 61722 - National Institute of Neurological Disorders and Stroke; Notice of Closed Meetings

    Science.gov (United States)

    2011-10-05

    ..., DHHS/NIH/NINDS/DER/SRB, 6001 Executive Boulevard, MSC 9529, Neuroscience Center, Room 3208, Bethesda...., Scientific Review Officer, DHHS/NIH/NINDS/DER/SRB, 6001 Executive Boulevard, MSC 9529, Neuroscience Center... Neurosciences, National Institutes of Health, HHS) Dated: September 27, 2011. Jennifer S. Spaeth,...

  5. Differentiation of Mesenchymal Stem Cells under Hypoxia and Normoxia: Lipid Profiles Revealed by Time-of-Flight Secondary Ion Mass Spectrometry and Multivariate Analysis

    NARCIS (Netherlands)

    Georgi, N.; Cillero-Pastor, B.; Eijkel, G.B.; Chinnagounder Periyasamy, P.; Kiss, A.; Blitterswijk, van C.A.; Post, J.N.; Heeren, R.M.A.; Karperien, H.B.J.

    2015-01-01

    Mesenchymal stem cells (MSC) have the ability to self-renew and differentiate into multiple cell types valuable for clinical treatment of rheumatic pathologies. To study the chondrogenic potential of MSC and identify the conditions that recreate the native cartilage environment, we used time-of-flig

  6. Human adipose-tissue derived mesenchymal stem cells induce functional de-novo regulatory T cells with methylated FOXP3 gene DNA

    NARCIS (Netherlands)

    Engela, A. U.; Hoogduijn, M. J.; Boer, K.; Litjens, N. H. R.; Betjes, M. G. H.; Weimar, W.; Baan, C. C.

    2013-01-01

    Due to their immunomodulatory properties, mesenchymal stem cells (MSC) are interesting candidates for cellular therapy for autoimmune disorders, graft-versus-host disease and allograft rejection. MSC inhibit the proliferation of effector T cells and induce T cells with a regulatory phenotype. So far

  7. Differentiation of Bone Marrow: Derived Mesenchymal Stem Cells into Hepatocyte-like Cells.

    Science.gov (United States)

    Al Ghrbawy, Nesrien M; Afify, Reham Abdel Aleem Mohamed; Dyaa, Nehal; El Sayed, Asmaa A

    2016-09-01

    Cirrhosis is the end-stage liver fibrosis, whereby normal liver architecture is disrupted by fibrotic bands, parenchymal nodules and vascular distortion. Portal hypertension and hepatocyte dysfunction are the end results and give rise to major systemic complications and premature death. Mesenchymal stem cells (MSC) have the capacity of self-renew and to give rise to cells of various lineages, so MSC can be isolated from bone marrow (BM) and induced to differentiate into hepatocyte-like cells. MSC were induced to differentiate into hepatocyte-like cells by hepatotic growth factor (HGF) and fibroblast growth factor-4 (FGF-4). Differentiated cells were examined for the expression of hepatocyte-specific markers and hepatocyte functions. MSC were isolated. Flow cytometry analysis showed that they expressed the MSC-specific markers, reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that MSC expressed the hepatocyte-specific marker cytokeratin 18 (CK-18) following hepatocyte induction. This study demonstrates that BM-derived-MSC can differentiate into functional hepatocyte-like cells following the induction of HGF and FGF-4. MSC can serve as a favorable cell source for tissue engineering in the treatment of liver disease. PMID:27429519

  8. Local delivery of mesenchymal stem cells with poly-lactic-co-glycolic acid nano-fiber scaffold suppress arthritis in rats.

    Directory of Open Access Journals (Sweden)

    Xiangmei Zhang

    Full Text Available Mesenchymal stem cells (MSC have been used recently for the treatment of autoimmune diseases in murine animal models due to the immunoregulatory capacity. Current utilization of MSC requires cells in certain quantity with multiple courses of administration, leading to limitation in clinical usage. Here we efficiently treated collagen-induced arthritis rats with a single local implantation with reduced number of MSC (2∼20% of previous studies with nano-fiber poly-lactic-co-glycolic acid (nano-fiber scaffold. MSC seeded on nano-fiber scaffold suppressed arthritis and bone destruction due to inhibition of systemic inflammatory reaction and immune response by suppressing T cell proliferation and reducing anti- type II collagen antibody production. In vivo tracing of MSC demonstrated that these cells remained within the scaffold without migrating to other organs. Meanwhile, in vitro culture of MSC with nano-fiber scaffold significantly increased TGF-β1 production. These results indicate an efficient utilization of MSC with the scaffold for destructive joints in rheumatoid arthritis by a single and local inoculation. Thus, our data may serve as a new strategy for MSC-based therapy in inflammatory diseases and an alternative delivery method for bone destruction treatment.

  9. Intranasal mesenchymal stem cell treatment for neonatal brain damage : long-term cognitive and sensorimotor improvement

    NARCIS (Netherlands)

    Donega, Vanessa; van Velthoven, Cindy T J; Nijboer, Cora H; van Bel, Frank; Kas, Martien J H; Kavelaars, Annemieke; Heijnen, Cobi J

    2013-01-01

    Mesenchymal stem cell (MSC) administration via the intranasal route could become an effective therapy to treat neonatal hypoxic-ischemic (HI) brain damage. We analyzed long-term effects of intranasal MSC treatment on lesion size, sensorimotor and cognitive behavior, and determined the therapeutic wi

  10. Patient-Specific Age: The Other Side of the Coin in Advanced Mesenchymal Stem Cell Therapy.

    Science.gov (United States)

    Schimke, Magdalena M; Marozin, Sabrina; Lepperdinger, Günter

    2015-01-01

    Multipotential mesenchymal stromal cells (MSC) are present as a rare subpopulation within any type of stroma in the body of higher animals. Prominently, MSC have been recognized to reside in perivascular locations, supposedly maintaining blood vessel integrity. During tissue damage and injury, MSC/pericytes become activated, evade from their perivascular niche and are thus assumed to support wound healing and tissue regeneration. In vitro MSC exhibit demonstrated capabilities to differentiate into a wide variety of tissue cell types. Hence, many MSC-based therapeutic approaches have been performed to address bone, cartilage, or heart regeneration. Furthermore, prominent studies showed efficacy of ex vivo expanded MSC to countervail graft-vs.-host-disease. Therefore, additional fields of application are presently conceived, in which MSC-based therapies potentially unfold beneficial effects, such as amelioration of non-healing conditions after tendon or spinal cord injury, as well as neuropathies. Working along these lines, MSC-based scientific research has been forged ahead to prominently occupy the clinical stage. Aging is to a great deal stochastic by nature bringing forth changes in an individual fashion. Yet, is aging of stem cells or/and their corresponding niche considered a determining factor for outcome and success of clinical therapies? PMID:26696897

  11. Patient-specific age: the other side of the coin in advanced mesenchymal stem cell therapy

    Directory of Open Access Journals (Sweden)

    Magdalena Maria Schimke

    2015-12-01

    Full Text Available Multipotential mesenchymal stromal cells (MSC are present as a rare subpopulation within any type of stroma in the body of higher animals. Prominently, MSC have been recognized to reside in perivascular locations, supposedly maintaining blood vessel integrity. During tissue damage and injury, MSC/pericytes become activated, evade from their perivascular niche and are thus assumed to support wound healing and tissue regeneration.In vitro MSC exhibit demonstrated capabilities to differentiate into a wide variety of tissue cell types. Hence, many MSC-based therapeutic approaches have been performed to address bone, cartilage or heart regeneration. Furthermore, prominent studies showed efficacy of ex vivo expanded MSC to countervail graft-versus-host-disease. Therefore, additional fields of application are presently conceived, in which MSC-based therapies potentially unfold beneficial effects, such as amelioration of non-healing conditions after tendon or spinal cord injury, as well as neuropathies. Working along these lines, MSC-based scientific research has been forged ahead to prominently occupy the clinical stage.Aging is to a great deal stochastic by nature bringing forth changes in an individual fashion. Yet, is aging of stem cells or/and their corresponding niche considered a determining factor for outcome and success of clinical therapies?

  12. 76 FR 66075 - Center for Scientific Review Notice of Closed Meetings

    Science.gov (United States)

    2011-10-25

    ... Rockledge Drive, Room 5208, MSC 7852, Bethesda, MD 20892, (301) 435-1165, walkermc@csr.nih.gov . Name of Committee: AIDS and Related Research Integrated Review Group HIV/AIDS Vaccines Study Section. Date: November..., Room 5208, MSC 7852, Bethesda, MD 20892, (301) 435-1165, walkermc@csr.nih.gov . Name of...

  13. 78 FR 64224 - Center for Scientific Review; Notice of Closed Meetings

    Science.gov (United States)

    2013-10-28

    ... 20892, 301-435-2365, aitouchea@csr.nih.gov . Name of Committee: AIDS and Related Research Integrated... Health, 6701 Rockledge Drive, Room 4138, MSC 7814, Bethesda, MD 20892, 301-435-1195, Chengy5@csr.nih.gov... 5218, MSC 7852, Bethesda, MD 20892, 301-806- 6596, rubertm@csr.nih.gov . Name of Committee: Center...

  14. Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells

    Science.gov (United States)

    Ersek, Adel; Pixley, John S.; Goodrich, A. Daisy; Porada, Christopher D.; Almeida-Porada, Graca; Thain, David S.; Zanjani, Esmail D.

    2010-01-01

    Objective To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero. Methods A three-step culture system was established for generating human fetal pMSC. Sheep fetuses were transplanted during the fetal transplant receptivity period with human pMSC and evaluated for in situ and functional engraftment in their pancreas, liver and bone marrow. Results Isolation and expansion of adherent cells from the human fetal pancreas yielded a cell population with morphologic and phenotypic characteristics similar to MSC derived from bone marrow. This putative stem cell population could undergo multilineage differentiation in vitro. Three to 27 months after fetal transplantation, the pancreatic engraftment frequency (chimeric index) was 79% while functional engraftment was noted in 50% of transplanted sheep. Hepatic and marrow engraftment and expression was noted as well. Conclusion We have established a procedure for isolation of human fetal pMSC that display characteristics similar to bone marrow derived MSC. In vivo results suggest the pMSC engraft, differentiate and secrete human insulin from the sheep pancreas. PMID:20170708

  15. Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

    DEFF Research Database (Denmark)

    Rasmussen, Jeppe Grøndahl; Frøbert, Ole; Pilgaard, Linda;

    2011-01-01

    Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic...

  16. Human Adipose Tissue-Derived Mesenchymal Stem Cells Abrogate Plasmablast Formation and Induce Regulatory B Cells Independently of T Helper Cells

    NARCIS (Netherlands)

    Franquesa, M.; Mensah, F. K.; Huizinga, R.; Strini, T.; Boon, L.; Lombardo, E.; DelaRosa, O.; Laman, J. D.; Grinyo, J. M.; Weimar, W.; Betjes, M. G. H.; Baan, C. C.; Hoogduijn, M. J.

    2015-01-01

    Mesenchymal or stromal stem cells (MSC) interact with cells of the immune system in multiple ways. Modulation of the immune system by MSC is believed to be a therapeutic option for autoimmune disease and transplant rejection. In recent years, B cells have moved into the focus of the attention as tar

  17. Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected 111In-labeled human mesenchymal stromal cells

    DEFF Research Database (Denmark)

    Lyngbaek, Stig; Ripa, Rasmus S; Haack-Sørensen, Mandana;

    2010-01-01

    This pilot trial aimed to investigate the utilization of (111)In-labeling of mesenchymal stromal cells (MSC) for in vivo tracking after intramyocardial transplantation in a xenotransplantation model with gender mismatched cells. Human male MSC were expanded ex vivo and labeled with (111)In...

  18. Serial in vivo imaging of the porcine heart after percutaneous, intramyocardially injected (111)In-labeled human mesenchymal stromal cells

    DEFF Research Database (Denmark)

    Lyngbæk, Stig; Ripa, Rasmus Sejersten; Haack-Sørensen, Mandana;

    2009-01-01

    This pilot trial aimed to investigate the utilization of (111)In-labeling of mesenchymal stromal cells (MSC) for in vivo tracking after intramyocardial transplantation in a xenotransplantation model with gender mismatched cells. Human male MSC were expanded ex vivo and labeled with (111)In...

  19. Human Cardiac Mesenchymal Stromal Cells with CD105+CD34- Phenotype Enhance the Function of Post-Infarction Heart in Mice

    Science.gov (United States)

    Wiśniewska, Ewa; Jarosz-Biej, Magdalena; Smolarczyk, Ryszard; Cichoń, Tomasz; Głowala-Kosińska, Magdalena; Śliwka, Joanna; Garbacz, Marcin; Szczypior, Mateusz; Jaźwiec, Tomasz; Langrzyk, Agnieszka; Zembala, Michał; Szala, Stanisław

    2016-01-01

    Aims The aim of the present study was to isolate mesenchymal stromal cells (MSC) with CD105+CD34- phenotype from human hearts, and to investigate their therapeutic potential in a mouse model of hindlimb ischemia and myocardial infarction (MI). The study aimed also to investigate the feasibility of xenogeneic MSCs implantation. Methods and Results MSC isolated from human hearts were multipotent cells. Separation of MSC with CD105+CD34- phenotype limited the heterogeneity of the originally isolated cell population. MSC secreted a number of anti-inflammatory and proangiogenic cytokines (mainly IL-6, IL-8, and GRO). Human MSC were transplanted into C57Bl/6NCrl mice. Using the mouse model of hindlimb ischemia it was shown that human MSC treated mice demonstrated a higher capillary density 14 days after injury. It was also presented that MSC administrated into the ischemic muscle facilitated fast wound healing (functional recovery by ischemic limb). MSC transplanted into an infarcted myocardium reduced the post-infarction scar, fibrosis, and increased the number of blood vessels both in the border area, and within the post-infarction scar. The improvement of left ventricular ejection fraction was also observed. Conclusion In two murine models (hindlimb ischemia and MI) we did not observe the xenotransplant rejection. Indeed, we have shown that human cardiac mesenchymal stromal cells with CD105+CD34- phenotype exhibit therapeutic potential. It seems that M2 macrophages are essential for healing and repair of the post-infarcted heart. PMID:27415778

  20. 75 FR 65020 - Center for Scientific Review; Notice of Closed Meetings

    Science.gov (United States)

    2010-10-21

    ...-074: Program Project: Solid-State NMR Technologies for Membrane Protein Structure. Date: November 16... Institutes of Health, 6701 Rockledge Drive, Room 3182, MSC 7759, Bethesda, MD 20892, 301-594-3163, champoum... Institutes of Health, 6701 Rockledge Drive, Room 5208, MSC 7852, Bethesda, MD 20892, (301) 435-1165,...

  1. Mesenchymal Stem Cell-Derived Microvesicles Support Ex Vivo Expansion of Cord Blood-Derived CD34+ Cells

    Directory of Open Access Journals (Sweden)

    Hui Xie

    2016-01-01

    Full Text Available Mesenchymal stem cells (MSCs are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB to increase the relatively low number of HSPCs in CB. Findings increasingly suggest that MSC-derived microvesicles (MSC-MVs play an important role in the biological functions of their parent cells. We speculate that MSC-MVs may recapitulate the hematopoiesis-supporting effects of their parent cells. In the current study, we found MSC-MVs containing microRNAs that are involved in the regulation of hematopoiesis. We also demonstrated that MSC-MVs could improve the expansion of CB-derived mononuclear cells and CD34+ cells and generate a greater number of primitive progenitor cells in vitro. Additionally, when MSC-MVs were added to the CB-MSC coculture system, they could improve the hematopoiesis-supporting effects of MSCs. These findings highlight the role of MSC-MVs in the ex vivo expansion of CB, which may offer a promising therapeutic approach in CB transplantation.

  2. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells

    DEFF Research Database (Denmark)

    Dokkedahl, Karin Stenderup; Justesen, Jeannette; Clausen, Christian;

    2003-01-01

    -gal+) cells and mean telomere length in early-passage cells obtained from young and old donors. However, MSC from old donors exhibited accelerated senescence evidenced by increased number of SA beta-gal+ cells per PD as compared with young (4% per PD vs 0.4% per PD, respectively). MSC from young and old...

  3. Mesenchymal stem cell therapy for heart disease.

    Science.gov (United States)

    Gnecchi, Massimiliano; Danieli, Patrizia; Cervio, Elisabetta

    2012-08-19

    Mesenchymal stem cells (MSC) are adult stem cells with capacity for self-renewal and multi-lineage differentiation. Initially described in the bone marrow, MSC are also present in other organs and tissues. From a therapeutic perspective, because of their easy preparation and immunologic privilege, MSC are emerging as an extremely promising therapeutic agent for tissue regeneration and repair. Studies in animal models of myocardial infarction have demonstrated the ability of transplanted MSC to engraft and differentiate into cardiomyocytes and vascular cells. Most importantly, engrafted MSC secrete a wide array of soluble factors that mediate beneficial paracrine effects and may greatly contribute to cardiac repair. Together, these properties can be harnessed to both prevent and reverse remodeling in the ischemically injured ventricle. In proof-of-concept and phase I clinical trials, MSC therapy improved left ventricular function, induced reverse remodeling, and decreased scar size. In this review we will focus on the current understanding of MSC biology and MSC mechanism of action in cardiac repair. PMID:22521741

  4. Priming Adipose-Derived Mesenchymal Stem Cells with Hyaluronan Alters Growth Kinetics and Increases Attachment to Articular Cartilage

    Directory of Open Access Journals (Sweden)

    Peter Succar

    2016-01-01

    Full Text Available Background. Biological therapeutics such as adipose-derived mesenchymal stem cell (MSC therapy are gaining acceptance for knee-osteoarthritis (OA treatment. Reports of OA-patients show reductions in cartilage defects and regeneration of hyaline-like-cartilage with MSC-therapy. Suspending MSCs in hyaluronan commonly occurs in animals and humans, usually without supporting data. Objective. To elucidate the effects of different concentrations of hyaluronan on MSC growth kinetics. Methods. Using a range of hyaluronan concentrations, we measured MSC adherence and proliferation on culture plastic surfaces and a novel cartilage-adhesion assay. We employed time-course and dispersion imaging to assess MSC binding to cartilage. Cytokine profiling was also conducted on the MSC-secretome. Results. Hyaluronan had dose-dependent effects on growth kinetics of MSCs at concentrations of entanglement point (1 mg/mL. At higher concentrations, viscosity effects outweighed benefits of additional hyaluronan. The cartilage-adhesion assay highlighted for the first time that hyaluronan-primed MSCs increased cell attachment to cartilage whilst the presence of hyaluronan did not. Our time-course suggested patients undergoing MSC-therapy for OA could benefit from joint-immobilisation for up to 8 hours. Hyaluronan also greatly affected dispersion of MSCs on cartilage. Conclusion. Our results should be considered in future trials with MSC-therapy using hyaluronan as a vehicle, for the treatment of OA.

  5. The effect of marrow mesenchymal stem cell transplantation on pulmonary fibrosis in rats

    Institute of Scientific and Technical Information of China (English)

    黄坤

    2012-01-01

    Objective To study the possible mechanisms of marrow mesenchymal stem cells(MSC) in therapy of bleomycin(BLM)-induced pulmonary fibrosis in rats. Methods Fifty-four female Wistar rats were randomly divided into a control group,a BLM group and a MSC group. The control group receivel intratracheal normal

  6. Enhanced Adipogenicity of Bone Marrow Mesenchymal Stem Cells in Aplastic Anemia

    Directory of Open Access Journals (Sweden)

    Naresh Kumar Tripathy

    2014-01-01

    Full Text Available Fatty bone marrow (BM and defective hematopoiesis are a pathologic hallmark of aplastic anemia (AA. We have investigated adipogenic and osteogenic potential of BM mesenchymal stem cells (BM-MSC in 10 AA patients (08 males and 02 females with median age of 37 years (range: 06 to 79 years and in the same number of age and sex matched controls. It was observed that BM-MSC of AA patients had a morphology, phenotype, and osteogenic differentiation potential similar to control subjects but adipocytes differentiated from AA BM-MSC had a higher density and larger size of lipid droplets and they expressed significantly higher levels of adiponectin and FABP4 genes and proteins as compared to control BM-MSC (P<0.01 for both. Thus our data shows that AA BM-MSC have enhanced adipogenicity, which may have an important implication in the pathogenesis of the disease.

  7. Human Stromal (Mesenchymal) Stem Cells from Bone Marrow, Adipose Tissue and Skin Exhibit Differences in Molecular Phenotype and Differentiation Potential

    DEFF Research Database (Denmark)

    Al-Nbaheen, May; Vishnubalaji, Radhakrishnan; Ali, Dalia;

    2013-01-01

    Human stromal (mesenchymal) stem cells (hMSCs) are multipotent stem cells with ability to differentiate into mesoderm-type cells e.g. osteoblasts and adipocytes and thus they are being introduced into clinical trials for tissue regeneration. Traditionally, hMSCs have been isolated from bone marrow......, but the number of cells obtained is limited. Here, we compared the MSC-like cell populations, obtained from alternative sources for MSC: adipose tissue and skin, with the standard phenotype of human bone marrow MSC (BM-MSCs). MSC from human adipose tissue (human adipose stromal cells (hATSCs)) and human skin......, MSC populations obtained from different tissues exhibit significant differences in their proliferation, differentiation and molecular phenotype, which should be taken into consideration when planning their use in clinical protocols....

  8. Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells

    DEFF Research Database (Denmark)

    Aggerholm-Pedersen, Ninna; Demuth, Christina; Safwat, Akmal;

    2016-01-01

    growth factor receptor (EGFR) was activated in both cell lines. However hMSC-TERT20-CE8 exhibited significantly higher expression of the EGFR ligands. EGFR inhibitors such as erlotinib and afatinib alone or in combination with doxorubicin failed to further decrease cell viability of hMSC-TERT20-CE8......) stem cell line hMSC-TERT4 and a transformed cell line hMSC-TERT20-CE8, known to form sarcoma-like tumours when implanted in immune-deficient mice, were used as models. Receptor tyrosine kinase (RTK) activation was analysed by RTK arrays and cellular viability after tyrosine kinases inhibitor (TKI....... However, inhibition with the TKI dasatinib in combination with doxorubicin decreased cell viability of the hMSC-TERT20-CE8 cell line. Conclusion. Our results demonstrate that dasatinib, but not EGFR-directed treatment, can decrease cell viability of stromal cancer stem cells less sensitive to doxorubicin....

  9. Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells: A Possible New Treatment Strategy

    DEFF Research Database (Denmark)

    Aggerholm-Pedersen, Ninna; Demouth, Christina; Safwat, Akmal;

    2016-01-01

    growth factor receptor (EGFR) was activated in both cell lines. However hMSC-TERT20-CE8 exhibited significantly higher expression of the EGFR ligands. EGFR inhibitors such as erlotinib and afatinib alone or in combination with doxorubicin failed to further decrease cell viability of hMSC-TERT20-CE8......) stem cell line hMSC-TERT4 and a transformed cell line hMSC-TERT20-CE8, known to form sarcoma-like tumours when implanted in immune-deficient mice, were used as models. Receptor tyrosine kinase (RTK) activation was analysed by RTK arrays and cellular viability after tyrosine kinases inhibitor (TKI....... However, inhibition with the TKI dasatinib in combination with doxorubicin decreased cell viability of the hMSC-TERT20-CE8 cell line. Conclusion. Our results demonstrate that dasatinib, but not EGFR-directed treatment, can decrease cell viability of stromal cancer stem cells less sensitive to doxorubicin....

  10. Learning Outcome Analysis: Analysing the Design of an Academic Programme%学习成果分析:一个教学计划设计的分析

    Institute of Scientific and Technical Information of China (English)

    CARROLL Dave

    2007-01-01

    This paper describes an analysis of the match between the module learning outcomes and the programme learning outcomes of the MSc in Assistive Technology offered by the School of Computing. The contribution of each MSc module to the overall programme learning outcomes was assessed using a Module Matrix. An overall Master Matrix was used to assess the overall contribution of all 16 modules to the programme learning outcomes of the MSc programme. The analysis of the MSc programme design was taken a stage further by graphing the learning outcomes of the overall programme against the individual module learning outcomes. This provided a useful tool for analysis of the MSc programme. The approach outlined in this paper provides a visualisation tool that allows the programme designer to quickly identify the deficiencies and excesses in the relationships between programme learning outcomes and module learning outcomes.

  11. [Recent Advances on the Immunoregulation of MicroRNA-155 in Mesenchymal Stem Cells--Review].

    Science.gov (United States)

    Han, Xiao; Wang, Lei; Wu, Tao; Bai, Hai

    2016-02-01

    Mesenchymal stem cells (MSC) are capable of immunosuppression and differentiating into multiple cell lineages. MSC, which are accessed easily and less side-effects, have been a source of seed cells in tissue-engineering and cell-therapy. However, the application of MSC are limited by their differentiation of instability and easy aging. MicroRNA-155 (miR-155) is one of microRNA, which has powerful regulatory potential in a wide variety of immune cells through degrading specific mRNA after transcription and inhibiting translation of the target genes. Following the research of miR-155 deeply, it has an indispensable role in the proliferation, differentiation and immunoregulation of MSC. This review discusses the current understandings for the role of miR-155 in MSC.

  12. Endothelial cells influence the osteogenic potential of bone marrow stromal cells

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    Arvidson Kristina

    2009-11-01

    Full Text Available Abstract Background Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering. The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC and human umbilical vein endothelial cells (EC could influence the osteogenic potential of MSC in osteogenic factor-free medium. Methods After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. The effects on osteogenic differentiation of adding EC to MSC was systematically tested by the using Superarray assay and results were confirmed with real-time PCR. Results Five days after the addition of EC to MSC in a ratio of 1:5 (EC/MSC significant increases in cell proliferation and cellular bridges between the two cell types were detected, as well as increased mRNA expression of alkaline phosphatase (ALP. This effect was greater than that seen with addition of osteogenic factors such as dexamethasone, ascorbic acid and β-glycerophosphate to the culture medium. The expression of transcription factor Runx2 was enhanced in MSC incubated with osteogenic stimulatory medium, but was not influenced by induction with EC. The expression of Collagen type I was not influenced by EC but the cells grown in the osteogenic factor-free medium exhibited higher expression than those cultured with osteogenic stimulatory medium. Conclusion These results show that co-culturing of EC and MSC for 5 days influences osteogenic differentiation of MSC, an effect that might be independent of Runx2, and enhances the production of ALP by MSC.

  13. Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy

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    Caroline Schmidt-Lucke

    2015-01-01

    Full Text Available Introduction. Mesenchymal stromal cells (MSC have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi. Methods. In 29 patients with n=23 or without n=6 CMi undergoing endomyocardial biopsies (EMB, transcardiac gradients (TCGs of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45−CD34−CD11b−CD73+CD90+ cells accounted for 0.010 [0.0025–0.048]%/peripheral mononuclear cell (PMNC and as CD45−CD34−CD11b−CD73+CD105+ cells for 0.019 [0.0026–0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% P<0.01 transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (P<0.05, multivariate analysis. A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45−CD34−CD90+CD105+ in EMB (r=-0.73, P<0.005. SDF-1α was the strongest predictor for increased MSC in EMB (P<0.005, multivariate analysis. Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation.

  14. Molecular characterisation of stromal populations derived from human embryonic stem cells: Similarities to immortalised bone marrow derived stromal stem cells

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    Linda Harkness

    2015-12-01

    Full Text Available Human bone marrow-derived stromal (skeletal stem cells (BM-hMSC are being employed in an increasing number of clinical trials for tissue regeneration. A limiting factor for their clinical use is the inability to obtain sufficient cell numbers. Human embryonic stem cells (hESC can provide an unlimited source of clinical grade cells for therapy. We have generated MSC-like cells from hESC (called here hESC-stromal that exhibit surface markers and differentiate to osteoblasts and adipocytes, similar to BM-hMSC. In the present study, we used microarray analysis to compare the molecular phenotype of hESC-stromal and immortalised BM-hMSC cells (hMSC-TERT. Of the 7379 genes expressed above baseline, only 9.3% of genes were differentially expressed between undifferentiated hESC-stromal and BM-hMSC. Following ex vivo osteoblast induction, 665 and 695 genes exhibited ≥2-fold change (FC in hESC-stromal and BM-hMSC, respectively with 172 genes common to both cell types. Functional annotation of significantly changing genes revealed similarities in gene ontology between the two cell types. Interestingly, genes in categories of cell adhesion/motility and epithelial–mesenchymal transition (EMT were highly enriched in hESC-stromal whereas genes associated with cell cycle processes were enriched in hMSC-TERT. This data suggests that while hESC-stromal cells exhibit a similar molecular phenotype to hMSC-TERT, differences exist that can be explained by ontological differences between these two cell types. hESC-stromal cells can thus be considered as a possible alternative candidate cells for hMSC, to be employed in regenerative medicine protocols.

  15. Instant stem cell therapy: Characterization and concentration of human mesenchymal stem cells in vitro

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    P Kasten

    2008-10-01

    Full Text Available In regenerative medicine, there is an approach to avoid expansion of the mesenchymal stem cell (MSC before implantation. The aim of this study was to compare methods for instant MSC therapy by use of a portable, automatic and closed system centrifuge that allows for the concentration of MSCs. The main outcome measures were the amount of MSCs per millilitre of bone marrow (BM, clusters of differentiation (CD, proliferation and differentiation capacities of the MSC. A volume reduction protocol was compared to the traditional laboratory methods of isolation using a Ficoll gradient and native BM. Fifty millilitres of BM were obtained from haematologically healthy male Caucasians (n=10, age 8 to 49 years. The number of colony forming units-fibroblast (CFU-F/ml BM was highest in the centrifuge volume reduction protocol, followed by the native BM (not significant, the centrifuge Ficoll (p=0.042 and the manual Ficoll procedure (p=0.001. The MSC of all groups could differentiate into the mesenchymal lineages without significant differences between the groups. The CD pattern was identical for all groups: CD13+; CD 44+; CD73 +; CD90+; CD105+; HLA-A,B,C+; CD14-; CD34-; CD45-; CD271-; HLA-DR-. In a further clinical pilot study (n=5 with 297 ml BM (SD 18.6, the volume reduction protocol concentrated the MSC by a factor of 14: there were 1.08 x 102 MSC/ml BM (standard deviation (SD 1.02 x 102 before concentration, 14.8 x 102 MSC/ ml BM (SD 12.4 x 102 after concentration, and on average 296 x 102 MSC (SD 248.9 x 102, range 86.4-691.5 x 102 were available for MSC therapy. The volume reduction protocol of the closed centrifuge allows for the highest concentration of the MSC, and therefore, is a promising candidate for instant stem cell therapy.

  16. Exosomes Secreted by Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Repair Critical-Sized Bone Defects through Enhanced Angiogenesis and Osteogenesis in Osteoporotic Rats

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    Qi, Xin; Zhang, Jieyuan; Yuan, Hong; Xu, Zhengliang; Li, Qing; Niu, Xin; Hu, Bin; Wang, Yang; Li, Xiaolin

    2016-01-01

    Bone defects caused by trauma, severe infection, tumor resection and skeletal abnormalities are common osteoporotic conditions and major challenges in orthopedic surgery, and there is still no effective solution to this problem. Consequently, new treatments are needed to develop regeneration procedures without side effects. Exosomes secreted by mesenchymal stem cells (MSCs) derived from human induced pluripotent stem cells (hiPSCs, hiPSC-MSC-Exos) incorporate the advantages of both MSCs and iPSCs with no immunogenicity. However, there are no reports on the application of hiPSC-MSC-Exos to enhance angiogenesis and osteogenesis under osteoporotic conditions. HiPSC-MSC-Exos were isolated and identified before use. The effect of hiPSC-MSC-Exos on the proliferation and osteogenic differentiation of bone marrow MSCs derived from ovariectomized (OVX) rats (rBMSCs-OVX) in vitro were investigated. In vivo, hiPSC-MSC-Exos were implanted into critical size bone defects in ovariectomized rats, and bone regeneration and angiogenesis were examined by microcomputed tomography (micro-CT), sequential fluorescent labeling analysis, microfil perfusion and histological and immunohistochemical analysis. The results in vitro showed that hiPSC-MSC-Exos enhanced cell proliferation and alkaline phosphatase (ALP) activity, and up-regulated mRNA and protein expression of osteoblast-related genes in rBMSCs-OVX. In vivo experiments revealed that hiPSC-MSC-Exos dramatically stimulated bone regeneration and angiogenesis in critical-sized calvarial defects in ovariectomized rats. The effect of hiPSC-MSC-Exos increased with increasing concentration. In this study, we showed that hiPSC-MSC-Exos effectively stimulate the proliferation and osteogenic differentiation of rBMSCs-OVX, with the effect increasing with increasing exosome concentration. Further analysis demonstrated that the application of hiPSC-MSC-Exos+β-TCP scaffolds promoted bone regeneration in critical-sized calvarial defects by

  17. Mesenchymal Stem Cells Do Not Prevent Antibody Responses against Human α-L-Iduronidase when Used to Treat Mucopolysaccharidosis Type I

    Science.gov (United States)

    Martin, Priscila Keiko Matsumoto; Stilhano, Roberta Sessa; Samoto, Vivian Yochiko; Takiya, Christina Maeda; Peres, Giovani Bravin; da Silva Michelacci, Yara Maria Correa; da Silva, Flavia Helena; Pereira, Vanessa Gonçalves; D'Almeida, Vânia; Marques, Fabio Luiz Navarro; Otake, Andreia Hanada; Chammas, Roger; Han, Sang Won

    2014-01-01

    Mucopolysaccharidosis type I (MPSI) is an autosomal recessive disease that leads to systemic lysosomal storage, which is caused by the absence of α-L-iduronidase (IDUA). Enzyme replacement therapy is recognized as the best therapeutic option for MPSI; however, high titers of anti-IDUA antibody have frequently been observed. Due to the immunosuppressant properties of MSC, we hypothesized that MSC modified with the IDUA gene would be able to produce IDUA for a long period of time. Sleeping Beauty transposon vectors were used to modify MSC because these are basically less-immunogenic plasmids. For cell transplantation, 4×106 MSC-KO-IDUA cells (MSC from KO mice modified with IDUA) were injected into the peritoneum of KO-mice three times over intervals of more than one month. The total IDUA activities from MSC-KO-IDUA before cell transplantation were 9.6, 120 and 179 U for the first, second and third injections, respectively. Only after the second cell transplantation, more than one unit of IDUA activity was detected in the blood of 3 mice for 2 days. After the third cell transplantation, a high titer of anti-IDUA antibody was detected in all of the treated mice. Anti-IDUA antibody response was also detected in C57Bl/6 mice treated with MSC-WT-IDUA. The antibody titers were high and comparable to mice that were immunized by electroporation. MSC-transplanted mice had high levels of TNF-alpha and infiltrates in the renal glomeruli. The spreading of the transplanted MSC into the peritoneum of other organs was confirmed after injection of 111In-labeled MSC. In conclusion, the antibody response against IDUA could not be avoided by MSC. On the contrary, these cells worked as an adjuvant that favored IDUA immunization. Therefore, the humoral immunosuppressant property of MSC is questionable and indicates the danger of using MSC as a source for the production of exogenous proteins to treat monogenic diseases. PMID:24642723

  18. Comparison of human sodium iodide symporter (hNIS) gene expression between lentiviral and adenoviral vectors in rat mesenchymal stem cell

    International Nuclear Information System (INIS)

    Quantitative comparison of transgene expression within stem cells between lentivirus and adenovirus-mediated delivery systems has not been done. Here, we evaluated the human sodium iodide symporter (hNIS) gene expression in rat mesenchymal stem cell (rMSC) transduced by lentivirus or adenovirus, and compared the hNIS expression quantitatively between the two delivery systems. Lentiviral-mediated stably hNIS expressing rMSC (lenti-hNIS-rMSC) was constructed by cloning the hNIS gene into pLenti6/UbC/V5-DEST (Invitrogen) to obtain pLenti-hNIS, transducing rMSC with the pLenti-hNIS, and selecting with blasticidin for 3 weeks. Recombinant adenovirus expressing hNIS gene (Rad-hNIS) was produced by homologous recombination and Rad-hNIS transduced rMSC (adeno-hNIS-rMSC) was evaluated for the hNIS expression 48 hours post infection at MOI 1, 5, 20, 50, and 100. The hNIS expression in lenti-hNIS-rMSC or adeno-hNIS-rMSC was assessed by immunocytochemistry, western blot, and I-125 uptake. Immunocytochemistry using mono-clonal anti-hNIS antibody revealed that intensity of hNIS immunoreactivity in lenti-hNIS-rMSC was greater than that in adeno-hNIS-rMSC at MOl 20 but lower than that at MOl 50. Western blot analysis also showed that lenti-hNIS-rMSC was intermediate between adeno-hNIS-rMSCs at MOl 20 and 50 in hNIS expression. However in vitro I-125 uptake test demonstrated that iodide uptake in lenti-hNIS-rMSC (297046659 picomole/106 cells) was greater than that in adeno-hNIS-rMSC at MOI 100 (61682134 picomole/106 cells). These results suggest that lentivirus mediated hNIS expression is greater in terms of hNIS function but lower in terms of hNIS protein amount than adenovirus mediated hNIS expression 48 hours post infection. Stem cell tracking using hNIS as a reporter gene should be conducted in consideration of relative viral efficiency of transgene expression

  19. Response to intravenous allogeneic equine cord-blood-derived mesenchymal stromal cells administered from chilled or frozen state in serum and protein free media

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    Lynn Brandon Williams

    2016-07-01

    Full Text Available Equine Mesenchymal stromal cells (MSC are commonly transported, chilled or frozen, to veterinary clinics. These MSC must remain viable and minimally affected by culture, transport, or injection processes. The safety of two carrier solutions developed for optimal viability and excipient use were evaluated in ponies, with and without allogeneic cord blood-derived (CB MSC. We hypothesized that neither the carrier solutions nor CB-MSC would elicit measurable changes in clinical, hematological, or biochemical parameters. In 9 ponies (study 1 a bolus of HypoThermosol® FRS (HTS-FRS, CryoStor® CS10 (CS10 or saline was injected IV (n=3/treatment. Study 2, following a one week washout period 5x107 pooled allogeneic CB-MSC were administered IV in HTS-FRS following 24h simulated chilled transport. Study 3, following another one week washout period 5x107 pooled allogeneic CB-MSC were administered IV in CS10 immediately after thawing. Nine ponies received CB-MSCs in study 2 and 3 and three ponies received the cell carrier media without cells. CB-MSCs were pooled in equal numbers from five unrelated donors. In all studies ponies were monitored with physical examination, and blood collection for 7 days following injection. CD4 and CD8 lymphocyte populations were also evaluated in each blood sample.In all three studies, physical exam, complete blood cell count, serum biochemistry, and coagulation panel did not deviate from established normal ranges. Proportions of CD4+ and CD8+ lymphocytes increased at 168h post injection in CB-MSC treatment groups regardless of the carrier solution. Decreases in CD4+/CD8+ double positive populations were observed at 24 h and 72 h in CB-MSC treated animals. There was no difference in viability between CB-MSC suspended in HTS-FRS or CS10.HTS-FRS and CS10 used for low volume excipient injection of MSC suspensions was not associated with short-term adverse reactions. HTS-FRS and CS10 both adequately maintain CB-MSC viability

  20. A microbial "bloom" at the onset of the Messinian Salinity Crisis in the Piedmont Basin (NW Italy)

    Science.gov (United States)

    Natalicchio, Marcello; Birgel, Daniel; Dela Pierre, Francesco; Lozar, Francesca; Liu, Xiaolei; Hinrichs, Kai-Uwe; Peckmann, Jörn

    2015-04-01

    The Messinian Salinity Crisis (MSC), a severe ecological crisis that occurred in the Mediterranean Basin about 6 myr ago, had drastic consequences for both freshwater and marine ecosystems (Roveri et al., 2014). The response of prokaryotes to the changing conditions at the MSC onset is virtually unknown. In a lipid biomarker study of sediments straddling the MSC onset from the Pollenzo section (NW Italy), we aim to evaluate the response of microbial communities at the transition from normal marine to extreme conditions. In this section, the advent of the MSC does not coincide with the deposition of gypsum; instead shales and intercalated carbonate-rich beds deposited. These deposits are considered as the deep water counterparts of gypsum layers that formed in the shallower parts of the basin (Dela Pierre et al., 2012). In both pre-MSC and MSC deposits, the molecular fossil inventory is sourced from all three domains of life and is mainly represented by isoprenoidal alcohols, fatty acids, sterols, long chain n-alkanes and n-alcohols. However, after the MSC onset, a sharp increase of the long chains n-alkanes, n-alcohols and fatty acids occurs, indicating a significant increase of terrigenous organic matter, most likely sourced by enhanced riverine runoff. Remarkably, this coincides with an increase of sterols (sitosterol and dinosterol), that are typically interpreted as markers of phytoplankton. The same strata contain filamentous fossils, which have been interpreted as remains of sulfide-oxidizing bacteria (cf., Dela Pierre et al., 2012). The basal MSC deposits are also typified by an increase of isoprenoidal alcohols, including glycerol dibiphytanyl glycerol tetraethers (GDGTs) and diphytanyl glycerol diethers (DGD). Caldarchaeol and crenarchaeol are the overall most abundant GDGTs. Their almost equal distribution in pre-MSC deposits suggests that they derived from planktic Thaumarchaeota, which are abundant picoplanktonic organisms in modern oceans. In contrast

  1. Mesenchymal stem cells do not prevent antibody responses against human α-L-iduronidase when used to treat mucopolysaccharidosis type I.

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    Priscila Keiko Matsumoto Martin

    Full Text Available Mucopolysaccharidosis type I (MPSI is an autosomal recessive disease that leads to systemic lysosomal storage, which is caused by the absence of α-L-iduronidase (IDUA. Enzyme replacement therapy is recognized as the best therapeutic option for MPSI; however, high titers of anti-IDUA antibody have frequently been observed. Due to the immunosuppressant properties of MSC, we hypothesized that MSC modified with the IDUA gene would be able to produce IDUA for a long period of time. Sleeping Beauty transposon vectors were used to modify MSC because these are basically less-immunogenic plasmids. For cell transplantation, 4×10(6 MSC-KO-IDUA cells (MSC from KO mice modified with IDUA were injected into the peritoneum of KO-mice three times over intervals of more than one month. The total IDUA activities from MSC-KO-IDUA before cell transplantation were 9.6, 120 and 179 U for the first, second and third injections, respectively. Only after the second cell transplantation, more than one unit of IDUA activity was detected in the blood of 3 mice for 2 days. After the third cell transplantation, a high titer of anti-IDUA antibody was detected in all of the treated mice. Anti-IDUA antibody response was also detected in C57Bl/6 mice treated with MSC-WT-IDUA. The antibody titers were high and comparable to mice that were immunized by electroporation. MSC-transplanted mice had high levels of TNF-alpha and infiltrates in the renal glomeruli. The spreading of the transplanted MSC into the peritoneum of other organs was confirmed after injection of 111In-labeled MSC. In conclusion, the antibody response against IDUA could not be avoided by MSC. On the contrary, these cells worked as an adjuvant that favored IDUA immunization. Therefore, the humoral immunosuppressant property of MSC is questionable and indicates the danger of using MSC as a source for the production of exogenous proteins to treat monogenic diseases.

  2. Efficient generation of multipotent mesenchymal stem cells from umbilical cord blood in stroma-free liquid culture.

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    Rowayda Peters

    Full Text Available BACKGROUND: Haematopoiesis is sustained by haematopoietic (HSC and mesenchymal stem cells (MSC. HSC are the precursors for blood cells, whereas marrow, stroma, bone, cartilage, muscle and connective tissues derive from MSC. The generation of MSC from umbilical cord blood (UCB is possible, but with low and unpredictable success. Here we describe a novel, robust stroma-free dual cell culture system for long-term expansion of primitive UCB-derived MSC. METHODS AND FINDINGS: UCB-derived mononuclear cells (MNC or selected CD34(+ cells were grown in liquid culture in the presence of serum and cytokines. Out of 32 different culture conditions that have been tested for the efficient expansion of HSC, we identified one condition (DMEM, pooled human AB serum, Flt-3 ligand, SCF, MGDF and IL-6; further denoted as D7 which, besides supporting HSC expansion, successfully enabled long-term expansion of stromal/MSC from 8 out of 8 UCB units (5 MNC-derived and 3 CD34(+ selected cells. Expanded MSC displayed a fibroblast-like morphology, expressed several stromal/MSC-related antigens (CD105, CD73, CD29, CD44, CD133 and Nestin but were negative for haematopoietic cell markers (CD45, CD34 and CD14. MSC stemness phenotype and their differentiation capacity in vitro before and after high dilution were preserved throughout long-term culture. Even at passage 24 cells remained Nestin(+, CD133(+ and >95% were positive for CD105, CD73, CD29 and CD44 with the capacity to differentiate into mesodermal lineages. Similarly we show that UCB derived MSC express pluripotency stem cell markers despite differences in cell confluency and culture passages. Further, we generated MSC from peripheral blood (PB MNC of 8 healthy volunteers. In all cases, the resulting MSC expressed MSC-related antigens and showed the capacity to form CFU-F colonies. CONCLUSIONS: This novel stroma-free liquid culture overcomes the existing limitation in obtaining MSC from UCB and PB enabling so far unmet

  3. Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice

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    Tatsuhito Himeno

    2013-01-01

    Full Text Available Background. Although pathological involvements of diabetic polyneuropathy (DPN have been reported, no dependable treatment of DPN has been achieved. Recent studies have shown that mesenchymal stem cells (MSCs ameliorate DPN. Here we demonstrate a differentiation of induced pluripotent stem cells (iPSCs into MSC-like cells and investigate the therapeutic potential of the MSC-like cell transplantation on DPN. Research Design and Methods. For induction into MSC-like cells, GFP-expressing iPSCs were cultured with retinoic acid, followed by adherent culture for 4 months. The MSC-like cells, characterized with flow cytometry and RT-PCR analyses, were transplanted into muscles of streptozotocin-diabetic mice. Three weeks after the transplantation, neurophysiological functions were evaluated. Results. The MSC-like cells expressed MSC markers and angiogenic/neurotrophic factors. The transplanted cells resided in hindlimb muscles and peripheral nerves, and some transplanted cells expressed S100β in the nerves. Impairments of current perception thresholds, nerve conduction velocities, and plantar skin blood flow in the diabetic mice were ameliorated in limbs with the transplanted cells. The capillary number-to-muscle fiber ratios were increased in transplanted hindlimbs of diabetic mice. Conclusions. These results suggest that MSC-like cell transplantation might have therapeutic effects on DPN through secreting angiogenic/neurotrophic factors and differentiation to Schwann cell-like cells.

  4. Multiple intravenous infusions of bone marrow mesenchymal stem cells reverse hyperglycemia in experimental type 2 diabetes rats.

    Science.gov (United States)

    Hao, Haojie; Liu, Jiejie; Shen, Jing; Zhao, Yali; Liu, Huilin; Hou, Qian; Tong, Chuan; Ti, Dongdong; Dong, Liang; Cheng, Yu; Mu, Yiming; Liu, Jianping; Fu, Xiaobing; Han, Weidong

    2013-07-01

    The worldwide rapid increase in diabetes poses a significant challenge to current therapeutic approaches. Single-dose mesenchymal stem cell (MSC) infusion ameliorates hyperglycemia but fails to restore normoglycemia in diabetic animals. We therefore hypothesized that multiple intravenous MSC infusions may reverse hyperglycemia in type 2 diabetes (T2D) rats. We administered serial allogenous bone-marrow derived MSC infusions (1 × 10(6)cells/infusion) via the tail vein once every 2 weeks to T2D rats, induced by high-fat diet and streptozocin (STZ) administration. Hyperglycemia decreased only transiently after a single infusion in early-phase (1 week) T2D rats, but approximated normal levels after at least three-time infusions. This normal blood level was maintained for at least 9 weeks. Serum concentrations of both insulin and C-peptide were dramatically increased after serial MSC infusions. Oral glucose tolerance tests revealed that glucose metabolism was significantly ameliorated. Immunofluorescence analysis of insulin/glucagon staining revealed the restoration of islet structure and number after multiple MSC treatments. When multiple-MSC treatment was initiated in late-phase (5 week) T2D rats, the results were slightly different. The results of this study suggested that a multiple-MSC infusion strategy offers a viable clinical option for T2D patients.

  5. Performance-enhanced mesenchymal stem cells via intracellular delivery of steroids

    Science.gov (United States)

    Ankrum, James A.; Dastidar, Riddhi G.; Ong, Joon Faii; Levy, Oren; Karp, Jeffrey M.

    2014-04-01

    Inadequate immunomodulatory potency of mesenchymal stem cells (MSC) may limit their therapeutic efficacy. We report glucocorticoid steroids augment MSC expression and activity of indoleamine-2,3-dioxygenase (IDO), a primary mediator of MSC immunomodulatory function. This effect depends on signaling through the glucocorticoid receptor and is mediated through up-regulation of FOXO3. Treatment of MSCs with glucocorticoids, budesonide or dexamethasone, enhanced IDO expression following IFN-γ stimulation in multiple donors and was able to restore IDO expression in over-passaged MSCs. As IDO enhancement was most notable when cells were continuously exposed to budesonide, we engineered MSC with budesonide loaded PLGA microparticles. MSC efficiently internalized budesonide microparticles and exhibited 4-fold enhanced IDO activity compared to budesonide preconditioned and naïve MSC, resulting in a 2-fold improvement in suppression of stimulated peripheral blood mononuclear cells in an IDO-dependent manner. Thus, the augmentation of MSC immune modulation may abrogate challenges associated with inadequate potency and enhance their therapeutic efficacy.

  6. Characterization and Application of a Disposable Rotating Bed Bioreactor for Mesenchymal Stem Cell Expansion

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    Anne Neumann

    2014-11-01

    Full Text Available Recruitment of mesenchymal stromal cells (MSC into the field of tissue engineering is a promising development since these cells can be expanded vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way (single-use disposable bioreactors. In this study, umbilical cord-derived MSC (UC-MSC were expanded in a disposable Z 2000 H bioreactor under dynamic conditions. Z was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence.

  7. Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Relieve Acute Myocardial Ischemic Injury

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    Yuanyuan Zhao

    2015-01-01

    Full Text Available This study is aimed at investigating whether human umbilical cord mesenchymal stem cell- (hucMSC- derived exosomes (hucMSC-exosomes have a protective effect on acute myocardial infarction (AMI. Exosomes were characterized under transmission electron microscopy and the particles of exosomes were further examined through nanoparticle tracking analysis. Exosomes (400 μg protein were intravenously administrated immediately following ligation of the left anterior descending (LAD coronary artery in rats. Cardiac function was evaluated by echocardiography and apoptotic cells were counted using TUNEL staining. The cardiac fibrosis was assessed using Masson’s trichrome staining. The Ki67 positive cells in ischemic myocardium were determined using immunohistochemistry. The effect of hucMSC-exosomes on blood vessel formation was evaluated through tube formation and migration of human umbilical vein endothelial cells (EA.hy926 cells. The results indicated that ligation of the LAD coronary artery reduced cardiac function and induced cardiomyocyte apoptosis. Administration of hucMSC-exosomes significantly improved cardiac systolic function and reduced cardiac fibrosis. Moreover, hucMSC-exosomes protected myocardial cells from apoptosis and promoted the tube formation and migration of EA.hy926 cells. It is concluded that hucMSC-exosomes improved cardiac systolic function by protecting myocardial cells from apoptosis and promoting angiogenesis. These effects of hucMSC-exosomes might be associated with regulating the expression of Bcl-2 family.

  8. Fatigue properties of alloy 718 overlay-coated with a Co-based X40 alloy by the Micro Spark Coating

    Science.gov (United States)

    Kamma, Ryohta; Sakaguchi, Motoki; Okazaki, Masakazu; Shimoda, Yukihiro; Uchiyama, Takehiko; Ochiai, Hiroyuki; Watanabe, Mitsutoshi

    Micro Spark Coating (MSC) has been developed as a new functional coating process for Ni-based superalloys used in advanced gas turbines. In this study, some metallurgical and mechanical properties of a MSC layer made of a Co-based wear resisting alloy (X40), and its influence on the high temperature fatigue properties of Ni-based superalloy, Alloy718, were investigated. Prior evaluation of the metallurgical and mechanical properties of the MSC layer that the cavity fraction of MSC layer significantly decreased during the thermal exposure period at 650°C associating with the generation of an oxide phase, progressive sintering and the subsequent increase in hardness and elastic modulus of MSC layer. However, at 480°C these changes were not significant even after 1000hrs exposure. It was found from the high temperature fatigue tests at 480°C and 650°C that the fatigue life of the specimen with MSC layer was almost comparable to that of bare Alloy718 specimen at 480°C, while at 650°C the life of the former was slightly longer than that of the latter. These results suggested that the MSC would have a potential to add a new function to Ni-based superalloy without a reduction in fatigue properties at elevated temperature.

  9. NOVEL APPROACH TO RESOLVE NETWORK SECURITY ISSUES IN IP-PBX IN CONVERGED ARCHITECTURE

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    MUNIR B. SAYYAD,

    2010-10-01

    Full Text Available This paper aims to examine the security issues in the IP-PBX (IP Private Branch Exchange .The Traces are taken on the live environment using Vendor ‘X’ CDMA MSC. The traces are also taken at SBC (Session Boarder Controller. The PBX services can be provided over IP via a SIP (session initiation protocol trunk provided by network operator. The SIP trunk is connected to a switch i.e. MSC. Thus IP connectivity is available at IP-PBX end. Now calls can be originated from IP-PBX which is terminating on a mobile handset registered with MSC. The problem arises when a dummy/blank/fake CLI (caller line identification is configured at IP-PBX. As MSC isdoing only part of routing depending upon called party number, such calls with fake CLI pass through MSC without any intervention. So called party and even MSC are unaware of real number of calling party. Similar security issue arises when IP-PBX sends dummy IP addresses of IP phones connected to IP-PBX. Thus conflict of IP addresses and or called party numbers creates a major security concern. These are important issues for interfacing IP with traditional wire line or wireless network. Such a security issue can be resolved by registering IP-PBX and its extension numbers with MSC. This paper describes the probable methods to resolve above issues.

  10. Mesenchymal Stem Cells Respond to Hypoxia by Increasing Diacylglycerols.

    Science.gov (United States)

    Lakatos, Kinga; Kalomoiris, Stefanos; Merkely, Béla; Nolta, Jan A; Fierro, Fernando A

    2016-02-01

    Mesenchymal stem cells (MSC) are currently being tested clinically for a plethora of conditions, with most approaches relying on the secretion of paracrine signals by MSC to modulate the immune system, promote wound healing, and induce angiogenesis. Hypoxia has been shown to affect MSC proliferation, differentiation, survival and secretory profile. Here, we investigate changes in the lipid composition of human bone marrow-derived MSC after exposure to hypoxia. Using mass spectrometry, we compared the lipid profiles of MSC derived from five different donors, cultured for two days in either normoxia (control) or hypoxia (1% oxygen). Hypoxia induced a significant increase of total triglycerides, fatty acids and diacylglycerols (DG). Remarkably, reduction of DG levels using the phosphatidylcholine-specific phospholipase C inhibitor D609 inhibited the secretion of VEGF and Angiopoietin-2, but increased the secretion of interleukin-8, without affecting significantly their respective mRNA levels. Functionally, incubation of MSC in hypoxia with D609 inhibited the potential of the cells to promote migration of human endothelial cells in a wound/scratch assay. Hence, we show that hypoxia induces in MSC an increase of DG that may affect the angiogenic potential of these cells. PMID:26212931

  11. Dasatinib and Doxorubicin Treatment of Sarcoma Initiating Cells: A Possible New Treatment Strategy

    Directory of Open Access Journals (Sweden)

    Ninna Aggerholm-Pedersen

    2016-01-01

    Full Text Available Background. One of the major challenges affecting sarcoma treatment outcome, particularly that of metastatic disease, is resistance to chemotherapy. Cancer-initiating cells are considered a major contributor to this resistance. Methods. An immortalised nontransformed human stromal (mesenchymal stem cell line hMSC-TERT4 and a transformed cell line hMSC-TERT20-CE8, known to form sarcoma-like tumours when implanted in immune-deficient mice, were used as models. Receptor tyrosine kinase (RTK activation was analysed by RTK arrays and cellular viability after tyrosine kinases inhibitor (TKI treatment with or without doxorubicin was assessed by MTS assay. Results. Initial results showed that the hMSC-TERT4 was more doxorubicin-sensitive while hMSC-TERT20-CE8 was less doxorubicin-sensitive evidenced by monitoring cell viability in the presence of doxorubicin at different doses. The epidermal growth factor receptor (EGFR was activated in both cell lines. However hMSC-TERT20-CE8 exhibited significantly higher expression of the EGFR ligands. EGFR inhibitors such as erlotinib and afatinib alone or in combination with doxorubicin failed to further decrease cell viability of hMSC-TERT20-CE8. However, inhibition with the TKI dasatinib in combination with doxorubicin decreased cell viability of the hMSC-TERT20-CE8 cell line. Conclusion. Our results demonstrate that dasatinib, but not EGFR-directed treatment, can decrease cell viability of stromal cancer stem cells less sensitive to doxorubicin.

  12. Identification of Pathways in Liver Repair Potentially Targeted by Secretory Proteins from Human Mesenchymal Stem Cells

    Directory of Open Access Journals (Sweden)

    Sandra Winkler

    2016-07-01

    Full Text Available Background: The beneficial impact of mesenchymal stem cells (MSC on both acute and chronic liver diseases has been confirmed, although the molecular mechanisms behind it remain elusive. We aim to identify factors secreted by undifferentiated and hepatocytic differentiated MSC in vitro in order to delineate liver repair pathways potentially targeted by MSC. Methods: Secreted factors were determined by protein arrays and related pathways identified by biomathematical analyses. Results: MSC from adipose tissue and bone marrow expressed a similar pattern of surface markers. After hepatocytic differentiation, CD54 (intercellular adhesion molecule 1, ICAM-1 increased and CD166 (activated leukocyte cell adhesion molecule, ALCAM decreased. MSC secreted different factors before and after differentiation. These comprised cytokines involved in innate immunity and growth factors regulating liver regeneration. Pathway analysis revealed cytokine-cytokine receptor interactions, chemokine signalling pathways, the complement and coagulation cascades as well as the Januskinase-signal transducers and activators of transcription (JAK-STAT and nucleotide-binding oligomerization domain-like receptor (NOD-like receptor signalling pathways as relevant networks. Relationships to transforming growth factor β (TGF-β and hypoxia-inducible factor 1-α (HIF1-α signalling seemed also relevant. Conclusion: MSC secreted proteins, which differed depending on cell source and degree of differentiation. The factors might address inflammatory and growth factor pathways as well as chemo-attraction and innate immunity. Since these are prone to dysregulation in most liver diseases, MSC release hepatotropic factors, potentially supporting liver regeneration.

  13. In Vivo therapeutic potential of mesenchymal stem cell-derived extracellular vesicles with optical imaging reporter in tumor mice model.

    Science.gov (United States)

    Kalimuthu, Senthilkumar; Gangadaran, Prakash; Li, Xiu Juan; Oh, Ji Min; Lee, Ho Won; Jeong, Shin Young; Lee, Sang-Woo; Lee, Jaetae; Ahn, Byeong-Cheol

    2016-01-01

    Mesenchymal stem cells (MSCs) can be used as a therapeutic armor for cancer. Extracellular vesicles (EVs) from MSCs have been evaluated for anticancer effects. In vivo targeting of EVs to the tumor is an essential requirement for successful therapy. Therefore, non-invasive methods of monitoring EVs in animal models are crucial for developing EV-based cancer therapies. The present study to develop bioluminescent EVs using Renilla luciferase (Rluc)-expressing MSCs. The EVs from MSC/Rluc cells (EV-MSC/Rluc) were visualized in a murine lung cancer model. The anticancer effects of EVs on Lewis lung carcinoma (LLC) and other cancer cells were assessed. EV-MSC/Rluc were visualized in vivo in the LLC-efffuc tumor model using optical imaging. The induction of apoptosis was confirmed with Annexin-V and propidium iodide staining. EV-MSC/Rluc and EV-MSCs showed a significant cytotoxic effect against LLC-effluc cells and 4T1; however, no significant effect on CT26, B16F10, TC1 cells. Moreover, EV-MSC/Rluc inhibited LLC tumor growth in vivo. EV-MSC/Rluc-mediated LLC tumor inhibitory mechanism revealed the decreased pERK and increased cleaved caspase 3 and cleaved PARP. We successfully developed luminescent EV-MSC/Rluc that have a therapeutic effect on LLC cells in both in vitro and in vivo. This bioluminescent EV system can be used to optimize EV-based therapy. PMID:27452924

  14. Allogeneic Mesenchymal Stem Cell Treatment Induces Specific Alloantibodies in Horses

    Directory of Open Access Journals (Sweden)

    Sean D. Owens

    2016-01-01

    Full Text Available Background. It is unknown whether horses that receive allogeneic mesenchymal stem cells (MSCs injections develop specific humoral immune response. Our goal was to develop and validate a flow cytometric MSC crossmatch procedure and to determine if horses that received allogeneic MSCs in a clinical setting developed measurable antibodies following MSC administration. Methods. Serum was collected from a total of 19 horses enrolled in 3 different research projects. Horses in the 3 studies all received unmatched allogeneic MSCs. Bone marrow (BM or adipose tissue derived MSCs (ad-MSCs were administered via intravenous, intra-arterial, intratendon, or intraocular routes. Anti-MSCs and anti-bovine serum albumin antibodies were detected via flow cytometry and ELISA, respectively. Results. Overall, anti-MSC antibodies were detected in 37% of the horses. The majority of horses (89% were positive for anti-bovine serum albumin (BSA antibodies prior to and after MSC injection. Finally, there was no correlation between the amount of anti-BSA antibody and the development of anti-MSC antibodies. Conclusion. Anti allo-MSC antibody development was common; however, the significance of these antibodies is unknown. There was no correlation between either the presence or absence of antibodies and the percent antibody binding to MSCs and any adverse reaction to a MSC injection.

  15. The effect of a chitosan-gelatin matrix and dexamethasone on the behavior of rabbit mesenchymal stem cells

    International Nuclear Information System (INIS)

    Cartilage tissue has poor capability of self-repair, especially in the case of severe cartilage damage due to trauma or age-related degeneration. Cell-based tissue engineering using scaffolds has provided an option for the repair of defects in adult cartilage tissue. Mesenchymal stem cells (MSC) and chondrocytes are the two major cell sources for cartilage tissue engineering. The present study combined culture conditions of MSC in a chitosan-gelatin matrix in chondrogenic media to evaluate their effects on MSC viability and chondrogenesis for cartilage tissue engineering. MSC were harvested from rabbit bone marrows and cultured in chondrogenic media supplemented, or not, with dexamethasone in a chitosan-gelatin film (C-GF). The association of C-GF and dexamethasone promoted significant increase in cell adhesivity, viability and proliferation when compared to MCS cultured in media without dexamethasone or C-GF. In addition, dexamethasone promoted increase in the collagen concentration of MSC cultures. A reduction of alkaline phosphatase activity after three weeks of culture in chondrogenic media was verified. No influence of the C-GF or of dexamethasone was observed in this matter. Therefore, it is reasonable to suggest that biomaterial-based chitosan-gelatin and chondrogenic media supplemented with dexamethasone may stimulate the proliferation and differentiation of MSC according to the complex environmental conditions. The information presented here should be useful for the development of biomaterials to regulate the chondrogenesis of MSC suitable for cartilage tissue engineering

  16. The effect of a chitosan-gelatin matrix and dexamethasone on the behavior of rabbit mesenchymal stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Medrado, G C B [Medicine School, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG (Brazil); Machado, C B [Biochemistry and Immunology Department, Biological Sciences Institute, UFMG - Federal University of Minas Gerais, mailbox 486, zip code 31270-901, Belo Horizonte, MG (Brazil); Valerio, P [Biochemistry and Immunology Department, Biological Sciences Institute, UFMG - Federal University of Minas Gerais, mailbox 486, zip code 31270-901, Belo Horizonte, MG (Brazil); Sanches, M D [Medicine School, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG (Brazil); Goes, A M [Biochemistry and Immunology Department, Biological Sciences Institute, UFMG - Federal University of Minas Gerais, mailbox 486, zip code 31270-901, Belo Horizonte, MG (Brazil)

    2006-09-15

    Cartilage tissue has poor capability of self-repair, especially in the case of severe cartilage damage due to trauma or age-related degeneration. Cell-based tissue engineering using scaffolds has provided an option for the repair of defects in adult cartilage tissue. Mesenchymal stem cells (MSC) and chondrocytes are the two major cell sources for cartilage tissue engineering. The present study combined culture conditions of MSC in a chitosan-gelatin matrix in chondrogenic media to evaluate their effects on MSC viability and chondrogenesis for cartilage tissue engineering. MSC were harvested from rabbit bone marrows and cultured in chondrogenic media supplemented, or not, with dexamethasone in a chitosan-gelatin film (C-GF). The association of C-GF and dexamethasone promoted significant increase in cell adhesivity, viability and proliferation when compared to MCS cultured in media without dexamethasone or C-GF. In addition, dexamethasone promoted increase in the collagen concentration of MSC cultures. A reduction of alkaline phosphatase activity after three weeks of culture in chondrogenic media was verified. No influence of the C-GF or of dexamethasone was observed in this matter. Therefore, it is reasonable to suggest that biomaterial-based chitosan-gelatin and chondrogenic media supplemented with dexamethasone may stimulate the proliferation and differentiation of MSC according to the complex environmental conditions. The information presented here should be useful for the development of biomaterials to regulate the chondrogenesis of MSC suitable for cartilage tissue engineering.

  17. Diminishing impairments in glucose uptake, mitochondrial content, and ADP-stimulated oxygen flux by mesenchymal stem cell therapy in the infarcted heart.

    Science.gov (United States)

    Hughey, Curtis C; James, Freyja D; Ma, Lianli; Bracy, Deanna P; Wang, Zhizhang; Wasserman, David H; Rottman, Jeffrey N; Shearer, Jane

    2014-01-01

    A constant provision of ATP is of necessity for cardiac contraction. As the heart progresses toward failure following a myocardial infarction (MI), it undergoes metabolic alterations that have the potential to compromise the ability to meet energetic demands. This study evaluated the efficacy of mesenchymal stem cell (MSC) transplantation into the infarcted heart to minimize impairments in the metabolic processes that contribute to energy provision. Seven and twenty-eight days following the MI and MSC transplantation, MSC administration minimized cardiac systolic dysfunction. Hyperinsulinemic-euglycemic clamps, coupled with 2-[(14)C]deoxyglucose administration, were employed to assess systemic insulin sensitivity and tissue-specific, insulin-mediated glucose uptake 36 days following the MI in the conscious, unrestrained, C57BL/6 mouse. The improved systolic performance in MSC-treated mice was associated with a preservation of in vivo insulin-stimulated cardiac glucose uptake. Conserved glucose uptake in the heart was linked to the ability of the MSC treatment to diminish the decline in insulin signaling as assessed by Akt phosphorylation. The MSC treatment also sustained mitochondrial content, ADP-stimulated oxygen flux, and mitochondrial oxidative phosphorylation efficiency in the heart. Maintenance of mitochondrial function and density was accompanied by preserved peroxisome proliferator-activated receptor-γ coactivator-1α, a master regulator of mitochondrial biogenesis. These studies provide insight into mechanisms of action that lead to an enhanced energetic state in the infarcted heart following MSC transplantation that may assist in energy provision and dampen cardiac dysfunction. PMID:24196528

  18. Mesenchymal Stromal Cells Do Not Increase the Risk of Viral Reactivation Nor the Severity of Viral Events in Recipients of Allogeneic Stem Cell Transplantation

    Directory of Open Access Journals (Sweden)

    Giovanna Lucchini

    2012-01-01

    Full Text Available Mesenchymal stromal cells (MSC are tested in clinical trials to treat graft versus host disease (GvHD after stem cell transplantation (SCT. In vitro studies demonstrated MSC's broad immunosuppressive activity. As infections represent a major risk after SCT, it is important to understand the role of MSC in this context. We analyzed 24 patients (pts receiving MSC for GvHD in our Unit between 2009 and 2011. We recorded viral reactivations as measured in whole blood with polymerase chain reaction for 100 days following MSC administration. In patients with a documented viral reactivation in the first 3 days following MSCs infusion the frequency of virus-specific IFNgamma-producing cells was determined through enzyme-linked immunospot assay. In our cohort of patients viral reactivation after MSC infusion occurred in 45% of the cases, which did not significantly differ from the incidence in a historical cohort of patients affected by steroid resistant GvHD and treated with conventional immunosuppression. No patient presented severe form of infection. Two cases could be checked for immunological response to viral stimulus and demonstrated virus specific T-cytotoxic lymphocyte activity. In our experience MSC infusion did not prove to trigger more frequent or severer viral reactivations in the post transplantation setting.

  19. Marijuana smoke condensate induces p53-mediated apoptosis in human lung epithelial cells.

    Science.gov (United States)

    Kim, Ha Ryong; Jung, Mi Hyun; Lee, Soo Yeun; Oh, Seung Min; Chung, Kyu Hyuck

    2013-01-01

    Since the largely abused worldwide used of marijuana, there have been many ongoing debates regarding the adverse health effects of marijuana smoking. Marijuana smoking was recently proved to cause pulmonary toxicity by inducing genotoxic effects or generating reactive oxygen species. Because p53, a tumor suppressor gene, has an important pathophysiologic role in the regulation of lung epithelial cell DNA damage responses, we hypothesized that p53 may be involved in the oxidative stress-mediated apoptosis induced by marijuana smoking. First, we confirmed that marijuana smoke condensate (MSC) induces oxidative stress in BEAS-2B cells. We observed that reactive oxygen species (ROS) generation was increased by MSC in the DCFH-DA assay. Also, antioxidant enzyme (superoxide dismutase, catalase) activity and their mRNA expressions were up-regulated by MSC. Second, we investigated p53 involvement in the MSC-induced apoptotic pathway in BEAS-2B cells. The results showed that MSC increased caspase-3 activation and DNA fragmentation as markers of apoptosis. In addition, the mRNA levels of apoptosis-related genes (p53 and Bax) were increased by MSC and phospho-p53, along with the increase of Bax protein expression by MSC. Apoptosis and apoptosis-related gene expression were partially blocked by an inhibitor of p53-dependent transcriptional activation (pifithrin-α). The results indicate that p53 plays a role in MSC-induced apoptosis. Taken together, the findings of the present study suggest that MSC partially induces p53-mediated apoptosis through ROS generation in human lung epithelial cells and this may have broader implications for our understanding of pulmonary diseases. PMID:23665932

  20. Mesenchymal stem cell therapy and lung diseases.

    Science.gov (United States)

    Akram, Khondoker M; Samad, Sohel; Spiteri, Monica; Forsyth, Nicholas R

    2013-01-01

    Mesenchymal stem cells (MSCs), a distinct population of adult stem cells, have amassed significant interest from both medical and scientific communities. An inherent multipotent differentiation potential offers a cell therapy option for various diseases, including those of the musculoskeletal, neuronal, cardiovascular and pulmonary systems. MSCs also secrete an array of paracrine factors implicated in the mitigation of pathological conditions through anti-inflammatory, anti-apoptotic and immunomodulatory mechanisms. The safety and efficacy of MSCs in human application have been confirmed through small- and large-scale clinical trials. However, achieving the optimal clinical benefit from MSC-mediated regenerative therapy approaches is entirely dependent upon adequate understanding of their healing/regeneration mechanisms and selection of appropriate clinical conditions. MSC-mediated acute alveolar injury repair. A cartoon depiction of an injured alveolus with associated inflammation and AEC apoptosis. Proposed routes of MSC delivery into injured alveoli could be by either intratracheal or intravenous routes, for instance. Following delivery a proposed mechanism of MSC action is to inhibit/reduce alveolar inflammation by abrogation of IL-1_-depenedent Tlymphocyte proliferation and suppression of TNF-_ secretion via macrophage activation following on from stimulation by MSC-secreted IL-1 receptor antagonist (IL-1RN). The inflammatory environment also stimulates MSC to secrete prostaglandin-E2 (PGE2) which can stimulate activated macrophages to secrete the anti-inflammatory cytokine IL-10. Inhibition of AEC apoptosis following injury can also be promoted via MSC stimulated up-regulation of the anti-apoptotic Bcl-2 gene. MSC-secreted KGF can stimulate AECII proliferation and migration propagating alveolar epithelial restitution. Alveolar structural engraftment of MSC is a rare event. PMID:22772131

  1. Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells

    International Nuclear Information System (INIS)

    Recent studies indicate that mesenchymal stromal cell (MSC) transplantation improves healing of injured and diseased skeletal muscle, although the mechanisms of benefit are poorly understood. In the present study, we investigated whether MSCs and/or their trophic factors were able to regulate matrix metalloproteinase (MMP) expression and activity in different cells of the muscle tissue. MSCs in co-culture with C2C12 cells or their conditioned medium (MSC-CM) up-regulated MMP-2 and MMP-9 expression and function in the myoblastic cells; these effects were concomitant with the down-regulation of the tissue inhibitor of metalloproteinases (TIMP)-1 and -2 and with increased cell motility. In the single muscle fiber experiments, MSC-CM administration increased MMP-2/9 expression in Pax-7+ satellite cells and stimulated their mobilization, differentiation and fusion. The anti-fibrotic properties of MSC-CM involved also the regulation of MMPs by skeletal fibroblasts and the inhibition of their differentiation into myofibroblasts. The treatment with SB-3CT, a potent MMP inhibitor, prevented in these cells, the decrease of α-smooth actin and type-I collagen expression induced by MSC-CM, suggesting that MSC-CM could attenuate the fibrogenic response through mechanisms mediated by MMPs. Our results indicate that growth factors and cytokines released by these cells may modulate the fibrotic response and improve the endogenous mechanisms of muscle repair/regeneration. - Highlights: • MSC-CM contains paracrine factors that up-regulate MMP expression and function in different skeletal muscle cells. • MSC-CM promotes myoblast and satellite cell migration, proliferation and differentiation. • MSC-CM negatively interferes with fibroblast-myoblast transition in primary skeletal fibroblasts. • Paracrine factors from MSCs modulate the fibrotic response and improve the endogenous mechanisms of muscle regeneration

  2. Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Sassoli, Chiara; Nosi, Daniele; Tani, Alessia; Chellini, Flaminia [Dept. of Experimental and Clinical Medicine—Section of Anatomy and Histology, University of Florence, Largo Brambilla, 3, 50134, Florence (Italy); Mazzanti, Benedetta [Dept. of Experimental and Clinical Medicine—Section of Haematology, University of Florence, Largo Brambilla, 3, 50134, Florence (Italy); Quercioli, Franco [CNR-National Institute of Optics (INO), Largo Enrico Fermi 6, 50125 Arcetri-Florence (Italy); Zecchi-Orlandini, Sandra [Dept. of Experimental and Clinical Medicine—Section of Anatomy and Histology, University of Florence, Largo Brambilla, 3, 50134, Florence (Italy); Formigli, Lucia, E-mail: formigli@unifi.it [Dept. of Experimental and Clinical Medicine—Section of Anatomy and Histology, University of Florence, Largo Brambilla, 3, 50134, Florence (Italy)

    2014-05-01

    Recent studies indicate that mesenchymal stromal cell (MSC) transplantation improves healing of injured and diseased skeletal muscle, although the mechanisms of benefit are poorly understood. In the present study, we investigated whether MSCs and/or their trophic factors were able to regulate matrix metalloproteinase (MMP) expression and activity in different cells of the muscle tissue. MSCs in co-culture with C2C12 cells or their conditioned medium (MSC-CM) up-regulated MMP-2 and MMP-9 expression and function in the myoblastic cells; these effects were concomitant with the down-regulation of the tissue inhibitor of metalloproteinases (TIMP)-1 and -2 and with increased cell motility. In the single muscle fiber experiments, MSC-CM administration increased MMP-2/9 expression in Pax-7{sup +} satellite cells and stimulated their mobilization, differentiation and fusion. The anti-fibrotic properties of MSC-CM involved also the regulation of MMPs by skeletal fibroblasts and the inhibition of their differentiation into myofibroblasts. The treatment with SB-3CT, a potent MMP inhibitor, prevented in these cells, the decrease of α-smooth actin and type-I collagen expression induced by MSC-CM, suggesting that MSC-CM could attenuate the fibrogenic response through mechanisms mediated by MMPs. Our results indicate that growth factors and cytokines released by these cells may modulate the fibrotic response and improve the endogenous mechanisms of muscle repair/regeneration. - Highlights: • MSC-CM contains paracrine factors that up-regulate MMP expression and function in different skeletal muscle cells. • MSC-CM promotes myoblast and satellite cell migration, proliferation and differentiation. • MSC-CM negatively interferes with fibroblast-myoblast transition in primary skeletal fibroblasts. • Paracrine factors from MSCs modulate the fibrotic response and improve the endogenous mechanisms of muscle regeneration.

  3. Human Mesenchymal Stem Cells Genetically Engineered to Overexpress Brain-derived Neurotrophic Factor Improve Outcomes in Huntington's Disease Mouse Models.

    Science.gov (United States)

    Pollock, Kari; Dahlenburg, Heather; Nelson, Haley; Fink, Kyle D; Cary, Whitney; Hendrix, Kyle; Annett, Geralyn; Torrest, Audrey; Deng, Peter; Gutierrez, Joshua; Nacey, Catherine; Pepper, Karen; Kalomoiris, Stefanos; D Anderson, Johnathon; McGee, Jeannine; Gruenloh, William; Fury, Brian; Bauer, Gerhard; Duffy, Alexandria; Tempkin, Theresa; Wheelock, Vicki; Nolta, Jan A

    2016-05-01

    Huntington's disease (HD) is a fatal degenerative autosomal dominant neuropsychiatric disease that causes neuronal death and is characterized by progressive striatal and then widespread brain atrophy. Brain-derived neurotrophic factor (BDNF) is a lead candidate for the treatment of HD, as it has been shown to prevent cell death and to stimulate the growth and migration of new neurons in the brain in transgenic mouse models. BDNF levels are reduced in HD postmortem human brain. Previous studies have shown efficacy of mesenchymal stem/stromal cells (MSC)/BDNF using murine MSCs, and the present study used human MSCs to advance the therapeutic potential of the MSC/BDNF platform for clinical application. Double-blinded studies were performed to examine the effects of intrastriatally transplanted human MSC/BDNF on disease progression in two strains of immune-suppressed HD transgenic mice: YAC128 and R6/2. MSC/BDNF treatment decreased striatal atrophy in YAC128 mice. MSC/BDNF treatment also significantly reduced anxiety as measured in the open-field assay. Both MSC and MSC/BDNF treatments induced a significant increase in neurogenesis-like activity in R6/2 mice. MSC/BDNF treatment also increased the mean lifespan of the R6/2 mice. Our genetically modified MSC/BDNF cells set a precedent for stem cell-based neurotherapeutics and could potentially be modified for other neurodegenerative disorders such as amyotrophic lateral sclerosis, Alzheimer's disease, and some forms of Parkinson's disease. These cells provide a platform delivery system for future studies involving corrective gene-editing strategies. PMID:26765769

  4. Mesenchymal bone marrow cell therapy in a mouse model of chagas disease. Where do the cells go?

    Directory of Open Access Journals (Sweden)

    Jasmin

    Full Text Available BACKGROUND: Chagas disease, resulting from infection with the parasite Trypanosoma cruzi (T. cruzi, is a major cause of cardiomyopathy in Latin America. Drug therapy for acute and chronic disease is limited. Stem cell therapy with bone marrow mesenchymal cells (MSCs has emerged as a novel therapeutic option for cell death-related heart diseases, but efficacy of MSC has not been tested in Chagas disease. METHODS AND RESULTS: We now report the use of cell-tracking strategies with nanoparticle labeled MSC to investigate migration of transplanted MSC in a murine model of Chagas disease, and correlate MSC biodistribution with glucose metabolism and morphology of heart in chagasic mice by small animal positron emission tomography (microPET. Mice were infected intraperitoneally with trypomastigotes of the Brazil strain of T. cruzi and treated by tail vein injection with MSC one month after infection. MSCs were labeled with near infrared fluorescent nanoparticles and tracked by an in vivo imaging system (IVIS. Our IVIS results two days after transplant revealed that a small, but significant, number of cells migrated to chagasic hearts when compared with control animals, whereas the vast majority of labeled MSC migrated to liver, lungs and spleen. Additionally, the microPET technique demonstrated that therapy with MSC reduced right ventricular dilation, a phenotype of the chagasic mouse model. CONCLUSIONS: We conclude that the beneficial effects of MSC therapy in chagasic mice arise from an indirect action of the cells in the heart rather than a direct action due to incorporation of large numbers of transplanted MSC into working myocardium.

  5. Human bone-marrow-derived mesenchymal stem cells

    DEFF Research Database (Denmark)

    Kassem, Moustapha; Abdallah, Basem M

    2008-01-01

    Mesenchymal stem cells (MSC) are a group of cells present in bone-marrow stroma and the stroma of various organs with the capacity for mesoderm-like cell differentiation into, for example, osteoblasts, adipocytes, and chondrocytes. MSC are being introduced in the clinic for the treatment...... of a variety of clinical conditions. The aim of this review is to provide an update regarding the biology of MSC, their identification and culture, and mechanisms controlling their proliferation and differentiation. We also review the current status of their clinical use. Areas in which research is needed...

  6. Addressing Library Anxiety (LA) in student nurses: a study in an NHS Foundation Trust Hospital library and information service.

    Science.gov (United States)

    Still, Madeleine

    2015-12-01

    Library anxiety is a concept which has been recognised in academic library circles since the early 1990s. It can result in students actively avoiding the library for the duration of their studies. Madeleine Still is Trust Librarian at North Tees & Hartlepool NHS Foundation Trust and while studying for an MSc, recognised that some student nurses were exhibiting signs of library anxiety. She decided to make it the focus of her MSc dissertation, and this article discusses her research project as well as highlighting the measures she has taken to address the issues she uncovered. Madeleine graduated in July 2013 with an MSc in Information & Library Studies from Robert Gordon University. PMID:26768906

  7. Comparison of Human Sodium/Iodide Symporter (hNIS) Gene Expressions between Lentiviral and Adenoviral Vectors in Rat Mesenchymal Stem Cells

    International Nuclear Information System (INIS)

    Quantitative comparison of transgene expression within stem cells between lentivirus and adenovirusmediated delivery systems has not been reported. Here, we evaluated the human sodium iodide symporter (hNIS) gene expression in rat mesenchymal stem cell (rMSC) transduced by lentivirus or adenovirus, and compared the hNIS expression quantitatively between the two delivery systems. Lentiviral-mediated hNIS expressing rMSC (lenti-hNIS-rMSC) was constructed by cloning hNIS gene into pLenti6/UbC/V5-DEST (Invitrogen) to obtain pLenti-hNIS, transducing rMSC with the pLenti-hNIS, and selecting with blasticidin for 3 weeks. Recombinant adenovirus expressing hNIS gene (Rad-hNIS) was produced by homologous recombination and transduction efficiency of Rad-hNIS into rMSC evaluated by Rad-GFP was 19.1±4.7%, 54.0±6.4%, 85.7±8.7%, and 98.4±1.3% at MOI 1, 5, 20, and 100, respectively. The hNIS expressions in lenti-hNIS-rMSC or adeno-hNIS-rMSC were assessed by immunocytochemistry, western blot, and I-125 uptake. Immunocytochemistry and western blot analyses revealed that hNIS expressions in lenti-hNIS-rMSC were greater than those in adeno-hNIS-rMSC at MOI 20 but lower than at MOI 50. However in vitro I-125 uptake test demonstrated that iodide uptake in lenti-hNIS-rMSC (29,704±6,659 picomole/106 cells) was greater than that in adeno-hNIS-rMSC at MOI 100 (6,168±2,134 picomole/106 cells). Despite lower amount of expressed protein, hNIS function in rMSC was greater by lentivirus than by adenovirus mediated expression. Stem cell tracking using hNIS as a reporter gene should be conducted in consideration of relative vector efficiency for transgene expression

  8. Comparison of Human Sodium/Iodide Symporter (hNIS) Gene Expressions between Lentiviral and Adenoviral Vectors in Rat Mesenchymal Stem Cells

    Energy Technology Data Exchange (ETDEWEB)

    Park, So Yeon; Lee, Won Woo; Kim, Hyun Joo; Chung, June Key; Kim, Sang Eun [Seoul National University College of Medicine, Seoul (Korea, Republic of); Kim, Sung Jin; Lee, Heui Ran [Medical Research Center, Seoul National University, Seoul (Korea, Republic of)

    2008-10-15

    Quantitative comparison of transgene expression within stem cells between lentivirus and adenovirusmediated delivery systems has not been reported. Here, we evaluated the human sodium iodide symporter (hNIS) gene expression in rat mesenchymal stem cell (rMSC) transduced by lentivirus or adenovirus, and compared the hNIS expression quantitatively between the two delivery systems. Lentiviral-mediated hNIS expressing rMSC (lenti-hNIS-rMSC) was constructed by cloning hNIS gene into pLenti6/UbC/V5-DEST (Invitrogen) to obtain pLenti-hNIS, transducing rMSC with the pLenti-hNIS, and selecting with blasticidin for 3 weeks. Recombinant adenovirus expressing hNIS gene (Rad-hNIS) was produced by homologous recombination and transduction efficiency of Rad-hNIS into rMSC evaluated by Rad-GFP was 19.1{+-}4.7%, 54.0{+-}6.4%, 85.7{+-}8.7%, and 98.4{+-}1.3% at MOI 1, 5, 20, and 100, respectively. The hNIS expressions in lenti-hNIS-rMSC or adeno-hNIS-rMSC were assessed by immunocytochemistry, western blot, and I-125 uptake. Immunocytochemistry and western blot analyses revealed that hNIS expressions in lenti-hNIS-rMSC were greater than those in adeno-hNIS-rMSC at MOI 20 but lower than at MOI 50. However in vitro I-125 uptake test demonstrated that iodide uptake in lenti-hNIS-rMSC (29,704{+-}6,659 picomole/10{sup 6} cells) was greater than that in adeno-hNIS-rMSC at MOI 100 (6,168{+-}2,134 picomole/10{sup 6} cells). Despite lower amount of expressed protein, hNIS function in rMSC was greater by lentivirus than by adenovirus mediated expression. Stem cell tracking using hNIS as a reporter gene should be conducted in consideration of relative vector efficiency for transgene expression.

  9. UNUSUAL PRESENTATION OF EXTRASKELETAL MESENCHYMAL CHONDROSARCOMA OF ABDOMEN IN 10 YR OLD GIRL- A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Ramani

    2013-01-01

    Full Text Available ABSTRACT: Mesenchymal chondrosarcoma (MSC is rare form of ch ondrosarcoma which usually arises in bone. Extraskeletal mesenchymal c hondrosarcomas are far less common and accounts approximately 30–50% of all mesenchymal chon drosarcomas. We report a rare case of intra-abdominal extraskeletal MSC in a 10 yr old gi rl who presented with biliary vomitings and abdominal pain. Ultrasound abdomen showed 42x24 mm h ypoechoic mass in right iliac fossa. CT abdomen showed calcified granulomas in spleen, Soft tissue dense lesion in right iliac fossa, suggestive of lymphadenopathy. Histopathology and immu nohistochemistry confirmed the diagnosis of MSC

  10. Design and analysis of palletized ISS Payloads

    Science.gov (United States)

    Lagoudas, Magdalini Z.; Boyle, David R.

    2000-01-01

    For development of commercial payloads integrated onto EXPRESS Pallet adapters, a wide variety of analysis tools are needed to properly assess the payload's mechanical, structural, and thermal characteristics. The simple transfer of solid model geometries from one of these analysis tools to another, though desired for effective concurrent engineering, can only occasionally be accomplished. The CSCE uses Pro/Engineer to develop the master payload geometry model. In that environment, MSC/NASTRAN and Pro/Mechanica achieve comparable accuracy in the structural analysis of relatively simple geometries; however, use of MSC/NASTRAN requires an additional model development step. For more complex geometries, MSC/NASTRAN offers significantly faster run times. .

  11. A42

    Directory of Open Access Journals (Sweden)

    V. Yuzhakov

    2015-11-01

    Reviewing the literature regarding the influence of MSC on the malignant growth revealed that this problem still remains quite unclear and disputable. One of the contradictions is their ability to have opposite effects on the repopulation activity of tumor cells. The complex interactions between MSC, tumor microenvironment and neoplastic cells seem to be crucial for the outcome of the oncological process development. Further detailed studies of the mechanisms of the cellular therapy using MSC on carcinogenesis are necessary to generate new insights into this area.

  12. How to Improve the Survival of Transplanted Mesenchymal Stem Cell in Ischemic Heart?

    Directory of Open Access Journals (Sweden)

    Liangpeng Li

    2016-01-01

    Full Text Available Mesenchymal stem cell (MSC is an intensely studied stem cell type applied for cardiac repair. For decades, the preclinical researches on animal model and clinical trials have suggested that MSC transplantation exerts therapeutic effect on ischemic heart disease. However, there remain major limitations to be overcome, one of which is the very low survival rate after transplantation in heart tissue. Various strategies have been tried to improve the MSC survival, and many of them showed promising results. In this review, we analyzed the studies in recent years to summarize the methods, effects, and mechanisms of the new strategies to address this question.

  13. Addressing Library Anxiety (LA) in student nurses: a study in an NHS Foundation Trust Hospital library and information service.

    Science.gov (United States)

    Still, Madeleine

    2015-12-01

    Library anxiety is a concept which has been recognised in academic library circles since the early 1990s. It can result in students actively avoiding the library for the duration of their studies. Madeleine Still is Trust Librarian at North Tees & Hartlepool NHS Foundation Trust and while studying for an MSc, recognised that some student nurses were exhibiting signs of library anxiety. She decided to make it the focus of her MSc dissertation, and this article discusses her research project as well as highlighting the measures she has taken to address the issues she uncovered. Madeleine graduated in July 2013 with an MSc in Information & Library Studies from Robert Gordon University.

  14. Immune Mechanisms of Mesenchymal Stem Cell Therapy for Acute Graft versus Host Disease

    OpenAIRE

    Tobin, Laura M.

    2012-01-01

    The aim of this work was to investigate the role of Mesenchymal stem cells (MSC) in the modulation of immune responses, focusing on suppression and induction of immune tolerance. To date, MSC therapy has proved beneficial for the treatment of inflammatory and autoimmune diseases, such as acute Graft versus Host Disease (aGvHD) and Crohn’s disease. However, the exact mechanisms of therapeutic benefit remain unclear. The key goals of this study were to identify the role of MSC derived soluble a...

  15. National Aeronautics and Space Administration Manned Spacecraft Center data base requirements study

    Science.gov (United States)

    1971-01-01

    A study was conducted to evaluate the types of data that the Manned Spacecraft Center (MSC) should automate in order to make available essential management and technical information to support MSC's various functions and missions. In addition, the software and hardware capabilities to best handle the storage and retrieval of this data were analyzed. Based on the results of this study, recommendations are presented for a unified data base that provides a cost effective solution to MSC's data automation requirements. The recommendations are projected through a time frame that includes the earth orbit space station.

  16. 小分子水凝胶结合腺病毒介导的肝细胞生长因子基因修饰对大鼠骨髓间充质干细胞生物学特性的影响%Effects of adenovirus transfection and small molecular hydrogels-mediated hepatocyte growth factor gene modification on biological characteristics of bone marrow mesenchymal stem cells in rats

    Institute of Scientific and Technical Information of China (English)

    区彩文; 陈国钦; 张建武; 邓菊; 吴智业; 陈敏生

    2013-01-01

    目的 观察小分子水凝胶(SMH)结合腺病毒(Ad)介导的肝细胞生长因子(HGF)基因修饰对大鼠骨髓间充质干细胞(MSC)生物学特性的影响.方法 取第9代MSC株进行实验,分为普通培养下MSC对照组(MSC组)、普通培养下转染Ad-HGF的MSC组(Ad-HGF+MSC组)、在SMH中培养的转染Ad-HGF的MSC组(SMH+Ad-HGF+MSC组)、在SMH中培养的MSC组(SMH+MSC组).将Ad-HGF转染MSC,48 h后流式细胞仪检测转染率.采用实时定量PCR检测MSC组、Ad-HGF+MSC组、SMH+Ad-HGF+MSC组细胞转染48 h后的mRNA水平.采用ELISA法测定Ad-HGF+MSC、MSC、Ad-EGFP+MSC、SMH+Ad-HGF+MSC组细胞上清液中HGF蛋白的含量,持续至转染后23 d.普通显微镜观察比较MSC组、SMH+MSC组、Ad-HGF+MSC组、SMH+Ad-HGF+MSC组细胞在转染继续培养7d后的形态.MTT法检测各组细胞1~7d的生长情况,绘制生长曲线.流式细胞仪检测转染2周后各组细胞表面标志物和细胞周期.各组细胞在5-aza诱导24 h后继续培养3周,免疫荧光化学检测细胞向心肌样细胞分化的能力情况.结果 Ad-HGF转染MSC 48 h后,转染率为74.7%.转染后48 h,MSC组Ct值为14.99;SMH+Ad-HGF+MSC组Ct值为8.38;Ad-HGF+MSC组Ct值为8.51;转染两组均出现明确的HGF基因表达.Ad-HGF+MSC组、SMH+Ad-HGF+MSC两组上清中均有HGF蛋白分泌,Ad-HGF+MSC组转染后48 h含量达最高峰,为121 μg/L,持续至23 d.SMH+Ad-HGF+MSC组HGF含量高峰在第3天,达118μg/L,持续至23 d.MSC组、Ad-HGF+MSC组细胞均呈成纤维细胞样生长,而转染或未转染Ad-HGF的MSC细胞在SMH水凝胶中三维培养时,细胞多为棒状,一些成球形,细胞之间的分支连接紧密,有聚集生长的趋势.Ad-HGF+MSC组与MSC组细胞生长曲线相似,各时间点吸光度A值差异无统计学意义,生长趋势吻合.SMH+MSC组与SMH+Ad-HGF+MSC组细胞生长速度前4d与MSC、Ad-HGF+MSC组比较,吸光度A值差异均无统计学意义;4d后细胞的吸光度A值则较Ad-HGF+MSC

  17. Territorio mediático-educativo en Suecia Media education landscape in Sweden

    Directory of Open Access Journals (Sweden)

    María Bergman

    2007-03-01

    Full Text Available Conocer el «territorio mediático educativo» en Suecia, a partir de la perspectiva presentada por la catedrática y pedagoga Birgitta Qvarsell, es el objetivo de este trabajo, que reflexiona sobre la ciencia pedagógica de los medios, «media educology». Basado en el contexto sueco, nos ofrece un análisis global relacionando el impacto y desarrollo de la técnica con los medios de comunicación, reseñando sus influencias en la pedagogía y la educación, contando para ello con todos los actores de este impacto mediático. El trabajo recoge también datos de un reciente informe del Consejo de Medios del Departamento de Cultura sueco, titulado «Los jóvenes y los medios». In this paper we describe the Swedish media education landscape and Brigitta Qvarsell‘s perspective on the influence of technological development in media education in Swedish context. Results of data collection about the media consumption by children and young Swedish people are also included. The conclusion remarks the need for a new area with ethnographic and semiotic basis: media educology.

  18. Väikelaevade sõiduohutus Eestis ja selle vastavus rahvusvahelistele nõuetele : [bakalaureusetöö] / Tanel Kukk ; Akadeemia Nord, õigusteaduskond ; juhendaja: Igor Gräzin

    Index Scriptorium Estoniae

    Kukk, Tanel

    2000-01-01

    Meresõiduohutus, sellega tegelevad organisatsioonid: IMO, MSC, SOLAS, Eesti Veeteede Amet. Väikelaevade sõiduohutust reguleeriv seadustik Eestis, rahvusvahelised väikelaevade sõiduohutuse eeskirjad

  19. CD146/MCAM defines functionality of human bone marrow stromal stem cell populations

    DEFF Research Database (Denmark)

    Harkness, Linda; Zaher, Walid; Ditzel, Nicholas;

    2016-01-01

    BACKGROUND: Identification of surface markers for prospective isolation of functionally homogenous populations of human skeletal (stromal, mesenchymal) stem cells (hMSCs) is highly relevant for cell therapy protocols. Thus, we examined the possible use of CD146 to subtype a heterogeneous h......MSC population. METHODS: Using flow cytometry and cell sorting, we isolated two distinct hMSC-CD146(+) and hMSC-CD146(-) cell populations from the telomerized human bone marrow-derived stromal cell line (hMSC-TERT). Cells were examined for differences in their size, shape and texture by using high......-content analysis and additionally for their ability to differentiate toward osteogenesis in vitro and form bone in vivo, and their migrational ability in vivo and in vitro was investigated. RESULTS: In vitro, the two cell populations exhibited similar growth rate and differentiation capacity to osteoblasts...

  20. Tumorigenic heterogeneity in cancer stem cells evolved from long-term cultures of telomerase-immortalized human mesenchymal stem cells

    DEFF Research Database (Denmark)

    Burns, Jorge S; Abdallah, Basem M; Guldberg, Per;

    2005-01-01

    Long-term cultures of telomerase-transduced adult human mesenchymal stem cells (hMSC) may evolve spontaneous genetic changes leading to tumorigenicity in immunodeficient mice (e.g., hMSC-TERT20). We wished to clarify whether this unusual phenotype reflected a rare but dominant subpopulation...... or if the stem cell origin allowed most cells to behave as cancer stem cells. Cultures of the hMSC-TERT20 strain at population doubling 440 were highly clonogenic (94%). From 110 single-cell clones expanded by 20 population doublings, 6 underwent detailed comparison. Like the parental population, each clone had...... tumorigenicity correlated with good viability plus capillary morphogenesis on serum starvation and high cyclin D1 expression. Thus, hMSC-TERT20 clones represent cancer stem cells with hierarchical tumorigenicity, providing new models to explore the stem cell hypothesis for cancer....

  1. National Institute on Deafness and Other Communication Disorders

    Science.gov (United States)

    ... Menu Home Health Info Hearing, Ear Infections, and Deafness Balance Taste and Smell Voice, Speech, and Language ... NIH… Turning Discovery Into Health ® National Institute on Deafness and Other Communication Disorders 31 Center Drive, MSC ...

  2. Experiment list: SRX084989 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress |...| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http:

  3. Experiment list: SRX101253 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 http

  4. Spasmodic Dysphonia

    Science.gov (United States)

    ... version Order free publications Find organizations Related Topics Autism Spectrum Disorder: Communication Problems in Children Hoarseness Taking ... NIH… Turning Discovery Into Health ® National Institute on Deafness and Other Communication Disorders 31 Center Drive, MSC ...

  5. Auditory Processing Disorder in Children

    Science.gov (United States)

    ... free publications Find organizations Related Topics Auditory Neuropathy Autism Spectrum Disorder: Communication Problems in Children Dysphagia Quick ... NIH… Turning Discovery Into Health ® National Institute on Deafness and Other Communication Disorders 31 Center Drive, MSC ...

  6. Communication Problems in Children with Autism Spectrum Disorder

    Science.gov (United States)

    ... NIH… Turning Discovery Into Health ® National Institute on Deafness and Other Communication Disorders 31 Center Drive, MSC 2320, Bethesda, MD USA 20892-2320 Email: nidcdinfo@nidcd.nih.gov NIDCD Intranet (Staff Only)

  7. Experiment list: SRX101213 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 http

  8. Migration of PKH26-1abeled mesenchymal stem cells in rats with Alzheimer's disease%PKH26标记的骨髓间质干细胞在阿尔茨海默病大鼠中的迁移

    Institute of Scientific and Technical Information of China (English)

    李文玉; 金日龙; 胡兴越

    2012-01-01

    目的:研究荧光染料PKH26标记的骨髓间质干细胞(bone-marrow derived mesenchymal stem cells,BM-MSC)在阿尔茨海默病(Alzheimer′s disease,以下简称AD)大鼠中的迁移.方法:抽取正常人骨髓,体外分离培养BM-MSC;以PKH26标记第5代BM-MSC,采用流式细胞仪检测BM-MSC表面标记物,并在荧光显微镜下观察PKH26标记率;将PKH26标记的BM-MSC尾静脉注射到正常对照组和AD动物模型组,14天后在大鼠海马区,用荧光显微镜观察PKH26标记的BM-MSC 细胞.利用Morris水迷宫实验比较AD模型组和BM-MSC移植组空间学习记忆能力.结果:流式细胞仪检测BM-MSC表面标记物CD73和CD105呈阳性,在体外BM-MSC的PKH26标记率达100%.Morris水迷宫实验比较BM-MSC移植组和AD动物组,BM-MSC移植组在第13、14天时空间学习记忆能力比AD动物组有显著改善.在移植BM-MSC的大鼠海马区可发现PKH26标记的BM-MSC阳性细胞,与DAPI吻合.PKH26阳性细胞在AD动物模型组明显多于正常对照组.结论:BM-MSC不仅能通过AD大鼠的血脑屏障,而且存活在AD大鼠的海马区,并有能够改善AD模型大鼠的学习障碍.%Objective: To investigate the migration of fluorescent dye PKH26 -labeled BM-MSC in the Alzheimer's model rats. Methods: Normal human bone marrow extracted for isolation of BM-MSC was cultured in vitro. The 5th passaged BM-MSC was labeled with PKH26,and observed under a fluorescence microscope for PKH26 labeling efficiency, and using flow cytometry BM-MSC surface markers was checked. The PKH26 labeled BM-MSC injected into the tail vein of the normal control group and AD animal model group, 14 days after finding the PKH26-labeled BM-MSC cells in the rat hippocampus using fluorescence microscopy. Using the Morris water maze experiment comparison of AD model and BM-MSC transplantation group of spatial learning and memory ability. Results: Flow cytometry showed BM-MSC surface markers CD73 and CD105 were positive. In vitro ,PKH26

  9. Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization

    DEFF Research Database (Denmark)

    Elsafadi, E; Manikandan, M; Dawud, RA;

    2016-01-01

    bone marrow-derived stromal (skeletal) stem cells (hMSC). siRNA-mediated gene silencing of TAGLN impaired lineage differentiation into osteoblasts and adipocytes but enhanced cell proliferation. Additional functional studies revealed that TAGLN deficiency impaired hMSC cell motility and in vitro...... transwell cell migration. On the other hand, TAGLN overexpression reduced hMSC cell proliferation, but enhanced cell migration, osteoblastic and adipocytic differentiation, and in vivo bone formation. In addition, deficiency or overexpression of TAGLN in hMSC was associated with significant changes...... in cellular and nuclear morphology and cytoplasmic organelle composition as demonstrated by high content imaging and transmission electron microscopy that revealed pronounced alterations in the distribution of the actin filament and changes in cytoskeletal organization. Molecular signature of TAGLN...

  10. Self-assembled composite matrix in a hierarchical 3-D scaffold for bone tissue engineering

    DEFF Research Database (Denmark)

    Chen, Muwan; Le, Dang Quang Svend; Baatrup, Anette;

    2011-01-01

    MSC seeding efficiency, proliferation, distribution and differentiation. Porous PCL meshes prepared by fused deposition modeling (FDM) were embedded in matrix of hyaluronic acid, methylated collagen and terpolymer via polyelectrolyte complex coacervation. Scaffolds were cultured statically and dynamically...

  11. Does 'Good' Cholesterol Matter in Heart Disease Risk?

    Science.gov (United States)

    ... is a member of the American College of Cardiology's Prevention of Cardiovascular Disease Section. "Many people know ... in the Journal of the American College of Cardiology . SOURCES: Dennis Ko, M.D., M.Sc., senior ...

  12. Experiment list: SRX101221 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 http

  13. Experiment list: SRX101247 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 htt

  14. Experiment list: SRX101214 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http

  15. Experiment list: SRX135213 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=5 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-3 || sample_term_id=EFO_0000586 htt

  16. Experiment list: SRX101232 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=5 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-4 || sample_term_id=EFO_0000586 http:

  17. Experiment list: SRX084995 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=6 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http:

  18. Experiment list: SRX084992 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http

  19. Experiment list: SRX081158 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=6 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 http:

  20. Experiment list: SRX101248 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 htt

  1. Experiment list: SRX101254 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=6 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http:

  2. Experiment list: SRX084988 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 htt

  3. Experiment list: SRX190757 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=5 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-3 || sample_term_id=EFO_0000586 http:

  4. Experiment list: SRX101225 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=5 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-3 || sample_term_id=EFO_0000586 http:

  5. Experiment list: SRX101223 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=5 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-4 || sample_term_id=EFO_0000586 http

  6. Experiment list: SRX101217 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=5 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-3 || sample_term_id=EFO_0000586 http

  7. Experiment list: SRX101261 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=5 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-3 || sample_term_id=EFO_0000586 http:

  8. Experiment list: SRX101245 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available onic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress ...|| PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 http

  9. Experiment list: SRX084984 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 htt

  10. Experiment list: SRX081163 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=6 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 http:

  11. Experiment list: SRX084985 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-2 || sample_term_id=EFO_0000586 htt

  12. Experiment list: SRX101262 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress || PASSAGE=5 || MEDIUM=Pub...lication in progress || SEX=Male || BATCH=MSC-4 || sample_term_id=EFO_0000586 http:

  13. Experiment list: SRX101244 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available yonic stem cell differentiated into mesenchymal stem cells || DIFFERENTIATION_METHOD=Publication in progress... || PASSAGE=6 || MEDIUM=Publication in progress || SEX=Male || BATCH=MSC-1 || sample_term_id=EFO_0000586 htt

  14. Study on phenotypic and cytogenetic characteristics of bone marrow mesenchymal stem cells in myelodysplastic syndromes

    Institute of Scientific and Technical Information of China (English)

    宋陆茜

    2013-01-01

    Objective To investigate phenotype,cell differentiation and cytogenetic properties of bone marrow(BM) mesenchymal stem cells(MSC)separated from the myelodysplastic syndrome(MDS) patients,and to analyze cytogenetic

  15. Hearing, Ear Infections, and Deafness

    Science.gov (United States)

    ... Noise and Hearing Loss Prevention - National Institute for Occupational Safety and Health Infographic: Hearing Loss and Hearing Aid ... Into Health ® National Institute on Deafness and Other Communication Disorders 31 Center Drive, MSC 2320, Bethesda, MD ...

  16. 75 FR 6675 - National Institute of Mental Health; Notice of Closed Meetings

    Science.gov (United States)

    2010-02-10

    ... Activities, National Institute of ] Mental Health, NIH, Neuroscience Center, 6001 Executive Blvd., Room 6151... Health, Neuroscience Center, 6001 Executive Boulevard, Rockville, MD 20852. (Telephone Conference Call... Institute of Mental Health, NIH, Neuroscience Center, 6001 Executive Blvd., Room 6154, MSC 9609,...

  17. 76 FR 69747 - National Institute of Neurological Disorders and Stroke; Notice of Closed Meetings

    Science.gov (United States)

    2011-11-09

    ... and evaluate grant applications. Place: National Institutes of Health, Neuroscience Center, 6001.../NIH/DHHS/Neuroscience Center, 6001 Executive Blvd., Suite 3208, MSC 9529, Bethesda, MD 20892, (301... review and evaluate grant applications. Place: National Institutes of Health, Neuroscience Center,...

  18. 77 FR 12603 - National Institute of Mental Health; Notice of Closed Meeting

    Science.gov (United States)

    2012-03-01

    ... evaluate grant applications. Place: National Institutes of Health, Neuroscience Center, 6001 Executive..., Neuroscience Center, 6001 Executive Blvd., Room 6140, MSC 9608, Bethesda, MD 20892-9608, 301-443-1225,...

  19. 78 FR 68075 - National Institute of Mental Health; Notice of Closed Meeting

    Science.gov (United States)

    2013-11-13

    ... evaluate grant applications. Place: National Institutes of Health, Neuroscience Center, 6001 Executive..., Neuroscience Center, 6001 Executive Blvd., Room 6149, MSC 9608, Bethesda, MD 20892-9608, 301-443-4525,...

  20. 76 FR 9586 - National Institute of Mental Health; Notice of Closed Meetings

    Science.gov (United States)

    2011-02-18

    .... Place: National Institutes of Health, Neuroscience Center, 6001 Executive Boulevard, Rockville, MD 20852... Extramural Activities, National Institute of Mental Health, NIH, Neuroscience Center/Room 6138/MSC 9608, 6001...: National Institutes of Health, Neuroscience Center, 6001 Executive Boulevard, Rockville, MD...