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Sample records for biosafety level bsl

  1. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures.

    Science.gov (United States)

    Janosko, Krisztina; Holbrook, Michael R; Adams, Ricky; Barr, Jason; Bollinger, Laura; Newton, Je T'aime; Ntiforo, Corrie; Coe, Linda; Wada, Jiro; Pusl, Daniela; Jahrling, Peter B; Kuhn, Jens H; Lackemeyer, Matthew G

    2016-10-03

    Biosafety level 4 (BSL-4) suit laboratories are specifically designed to study high-consequence pathogens for which neither infection prophylaxes nor treatment options exist. The hallmarks of these laboratories are: custom-designed airtight doors, dedicated supply and exhaust airflow systems, a negative-pressure environment, and mandatory use of positive-pressure ("space") suits. The risk for laboratory specialists working with highly pathogenic agents is minimized through rigorous training and adherence to stringent safety protocols and standard operating procedures. Researchers perform the majority of their work in BSL-2 laboratories and switch to BSL-4 suit laboratories when work with a high-consequence pathogen is required. Collaborators and scientists considering BSL-4 projects should be aware of the challenges associated with BSL-4 research both in terms of experimental technical limitations in BSL-4 laboratory space and the increased duration of such experiments. Tasks such as entering and exiting the BSL-4 suit laboratories are considerably more complex and time-consuming compared to BSL-2 and BSL-3 laboratories. The focus of this particular article is to address basic biosafety concerns and describe the entrance and exit procedures for the BSL-4 laboratory at the NIH/NIAID Integrated Research Facility at Fort Detrick. Such procedures include checking external systems that support the BSL-4 laboratory, and inspecting and donning positive-pressure suits, entering the laboratory, moving through air pressure-resistant doors, and connecting to air-supply hoses. We will also discuss moving within and exiting the BSL-4 suit laboratories, including using the chemical shower and removing and storing positive-pressure suits.

  2. Guidelines for Biosafety Training Programs for Workers Assigned to BSL-3 Research Laboratories.

    Science.gov (United States)

    Homer, Lesley C; Alderman, T Scott; Blair, Heather Ann; Brocard, Anne-Sophie; Broussard, Elaine E; Ellis, Robert P; Frerotte, Jay; Low, Eleanor W; McCarthy, Travis R; McCormick, Jessica M; Newton, JeT'Aime M; Rogers, Francine C; Schlimgen, Ryan; Stabenow, Jennifer M; Stedman, Diann; Warfield, Cheryl; Ntiforo, Corrie A; Whetstone, Carol T; Zimmerman, Domenica; Barkley, Emmett

    2013-03-01

    The Guidelines for Biosafety Training Programs for Workers Assigned to BSL-3 Research Laboratories were developed by biosafety professionals who oversee training programs for the 2 national biocontainment laboratories (NBLs) and the 13 regional biocontainment laboratories (RBLs) that participate in the National Institute of Allergy and Infectious Diseases (NIAID) NBL/RBL Network. These guidelines provide a general training framework for biosafety level 3 (BSL-3) high-containment laboratories, identify key training concepts, and outline training methodologies designed to standardize base knowledge, understanding, and technical competence of laboratory personnel working in high-containment laboratories. Emphasis is placed on building a culture of risk assessment-based safety through competency training designed to enhance understanding and recognition of potential biological hazards as well as methods for controlling these hazards. These guidelines may be of value to other institutions and academic research laboratories that are developing biosafety training programs for BSL-3 research.

  3. Establishment of Biosafety Level-3 (BSL-3 laboratory: Important criteria to consider while designing, constructing, commissioning & operating the facility in Indian setting

    Directory of Open Access Journals (Sweden)

    Devendra T Mourya

    2014-01-01

    This article describes the major steps involved in the process of construction of a BSL-3 laboratory in Indian settings, from freezing the concept of proposal to operationalization phase. The key to success of this kind of project is strong institutional commitment to biosafety norms, adequate fund availability, careful planning and designing, hiring good construction agency, monitoring by experienced consultancy agency and involvement of scientific and engineering personnel with biocontainment experience in the process.

  4. Biosafety Level 3 Recon Training

    Energy Technology Data Exchange (ETDEWEB)

    Dickens, Brian Scott [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Chavez, Melanie Ann [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Heimer, Donovan J. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Knudsen, Ryan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Velasquez, Celina Carmelita [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-04-12

    The Biosafety Level 3 Recon training is a 3D virtual tool developed for the Counter WMD Analysis Cell (CWAC) and the Asymmetric Warfare Group (AWG) by the Application Modeling and Development Team within the NEN-3 International Threat Reduction Group. The training simulates a situation where friendly forces have secured from hostile forces a suspected bioweapons development laboratory. The trainee is a squad member tasked to investigate the facility, locate laboratories within the facility, and identify hazards to entrants and the surrounding area. Before beginning the 3D simulation, the trainee must select the appropriate MOPP level for entering the facility. The items in the simulation, including inside and outside the bioweapon facility, are items that are commonly used by scientists in Biosafety Level (BSL) laboratories. Each item has clickable red tags that, when activated, give the trainee a brief description of the item and a controllable turn-around view. The descriptions also contain information about potential hazards the item can present. Trainees must find all tagged items in order to complete the simulation, but can also reference descriptions and turn-around view of the items in a glossary menu. Training is intended to familiarize individuals whom have little or no biology or chemistry background with technical equipment used in BSL laboratories. The revised edition of this simulation (Biosafety Level 3 Virtual Lab) changes the trainee into a investigator instead of a military combatant. Many doors now require a virtual badge swipe to open. Airlock doors may come in sets such that the open door must be closed before the next door in the set can be opened. A user interface was added so that the instructor can edit the information about the items (the brief descriptions mentioned above) using the simulation software instead of the previous method of manually entering the material in xml settings files. Facility labels, such as "No Parking" and "Men

  5. Framework for leadership and training of Biosafety Level 4 laboratory workers.

    Science.gov (United States)

    Le Duc, James W; Anderson, Kevin; Bloom, Marshall E; Estep, James E; Feldmann, Heinz; Geisbert, Joan B; Geisbert, Thomas W; Hensley, Lisa; Holbrook, Michael; Jahrling, Peter B; Ksiazek, Thomas G; Korch, George; Patterson, Jean; Skvorak, John P; Weingartl, Hana

    2008-11-01

    Construction of several new Biosafety Level 4 (BSL-4) laboratories and expansion of existing operations have created an increased international demand for well-trained staff and facility leaders. Directors of most North American BSL-4 laboratories met and agreed upon a framework for leadership and training of biocontainment research and operations staff. They agreed on essential preparation and training that includes theoretical consideration of biocontainment principles, practical hands-on training, and mentored on-the-job experiences relevant to positional responsibilities as essential preparation before a person's independent access to a BSL-4 facility. They also agreed that the BSL-4 laboratory director is the key person most responsible for ensuring that staff members are appropriately prepared for BSL-4 operations. Although standardized certification of training does not formally exist, the directors agreed that facility-specific, time-limited documentation to recognize specific skills and experiences of trained persons is needed.

  6. Evaluating the efficacy of hydrogen peroxide vapour against foot-and-mouth disease virus within a BSL4 biosafety facility.

    Science.gov (United States)

    Petit, B M; Almeida, F C; Uchiyama, T R; Lopes, F O C; Tino, K H; Chewins, J

    2017-10-01

    An evaluation was made of the efficacy of 35% hydrogen peroxide vapour (HPV) against foot-and-mouth disease virus (FMDV) in a biosafety facility. Biological indicators (BIs) were produced using three serotypes of FMDV, all with a titre of ≥10 6 TCID 50 per ml. Fifteen BIs of each serotype were distributed across five locations, throughout a 30-m 3 airlock chamber, producing a total of 45 BIs. Thirty-five percent HPV was generated and applied using a Bioquell vaporization module located in the centre of the chamber. After a dwell period of 40 min, the HPV was removed via the enclosures air handling system and the BIs were collected. The surfaces of the BIs were recovered into Glasgow's modified Eagle's medium (GMEM), cultivated in BHK21 Cl13 cell culture and analysed for evidence of cytopathic effect (CPE). No CPE was detected in any BI sample. Positive controls showed CPE. The experimentation shows that FMDV is susceptible to HPV decontamination and presents a potential alternative to formaldehyde. Foot-and-mouth disease virus (FMDV) is an important pathogen in terms of biosafety due to its infectious nature and wide range of host animals, such as cattle, sheep, goats and pigs. Outbreaks of FMDV can have a severe impact on livestock production, causing morbidity, mortality, reduced yields and trade embargoes. Laboratories studying FMDV must possess BSL4 robust bio-decontamination methods to prevent inadvertent release. Formaldehyde has been the primary agent for environmental decontamination, but its designation as a human carcinogen has led to a search for alternatives. This study shows 35% hydrogen peroxide vapour has the potential to be a rapid, effective, residue-free alternative. © 2017 The Society for Applied Microbiology.

  7. Practical biosafety in the tuberculosis laboratory: containment at the source is what truly counts

    NARCIS (Netherlands)

    Soolingen, D.; Wisselink, H.J.; Lumb, R.; Anthony, R.; Zanden, van der A.; Gilpin, C.

    2014-01-01

    In industrialised countries, sufficient resources for establishing and maintaining fully equipped biosafety level 3 (BSL-3) laboratories according to international standards are generally available. BSL-3 laboratories are designed to provide several layers of containment to protect the laboratory

  8. Biosafety and biosecurity measures: management of biosafety level 3 facilities.

    Science.gov (United States)

    Zaki, Adel N

    2010-11-01

    With the increasing biological threat from emerging infectious diseases and bioterrorism, it has become essential for governments around the globe to increase awareness and preparedness for identifying and containing those agents. This article introduces the basic concepts of laboratory management, laboratory biosafety and laboratory biosecurity. Assessment criteria for laboratories' biorisk should include both biosafety and biosecurity measures. The assessment requires setting specific goals and selecting management approaches. In order to implement technologies at the laboratory working level, a management team should be created whose role is to implement biorisk policies, rules and regulations appropriate for that facility. Rules and regulations required by government authorities are presented, with special emphasis on methods for air control, and liquid and solid waste management. Management and biorisk measures and appropriate physical facilities must keep pace, ensuring efficient facilities that protect workers, the environment, the product (research, diagnostic and/or vaccine) and the biological pathogen. Published by Elsevier B.V.

  9. Microbial Observatory (ISS-MO): Study of BSL-2 bacterial isolates from the International Space Station

    Data.gov (United States)

    National Aeronautics and Space Administration — In an on-going Microbial Observatory experimental investigation on the International Space Station (ISS) multiple bacterial isolates of Biosafety Level 2 (BSL-2)...

  10. Implementation of a personnel reliability program as a facilitator of biosafety and biosecurity culture in BSL-3 and BSL-4 laboratories.

    Science.gov (United States)

    Higgins, Jacki J; Weaver, Patrick; Fitch, J Patrick; Johnson, Barbara; Pearl, R Marene

    2013-06-01

    In late 2010, the National Biodefense Analysis and Countermeasures Center (NBACC) implemented a Personnel Reliability Program (PRP) with the goal of enabling active participation by its staff to drive and improve the biosafety and biosecurity culture at the organization. A philosophical keystone for accomplishment of NBACC's scientific mission is simultaneous excellence in operations and outreach. Its personnel reliability program builds on this approach to: (1) enable and support a culture of responsibility based on human performance principles, (2) maintain compliance with regulations, and (3) address the risk associated with the insider threat. Recently, the Code of Federal Regulations (CFR) governing use and possession of biological select agents and toxins (BSAT) was amended to require a pre-access suitability assessment and ongoing evaluation for staff accessing Tier 1 BSAT. These 2 new requirements are in addition to the already required Federal Bureau of Investigation (FBI) Security Risk Assessment (SRA). Two years prior to the release of these guidelines, NBACC developed its PRP to supplement the SRA requirement as a means to empower personnel and foster an operational environment where any and all work with BSAT is conducted in a safe, secure, and reliable manner.

  11. Biosafety

    Directory of Open Access Journals (Sweden)

    Ruhtan Baskaya

    2009-04-01

    Full Text Available Food safety, as well as the human, animal, and plant health are the sectors which are covered wihtin the framework of biosafety. Biosafety covers all the effots spent for the formulation of the policies and legal arrangentments related with the above mentioned sectors. The concept of biosafety involves the production of safe food, development and supply of Genetically Modified Organisms (GMO and the drugs used in combating and curing of plant and animal diseases, as well as their derivates, and management (control of the invasive alien species and their genotypes. [TAF Prev Med Bull 2009; 8(2.000: 177-186

  12. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices.

    Science.gov (United States)

    Mazur, Steven; Holbrook, Michael R; Burdette, Tracey; Joselyn, Nicole; Barr, Jason; Pusl, Daniela; Bollinger, Laura; Coe, Linda; Jahrling, Peter B; Lackemeyer, Matthew G; Wada, Jiro; Kuhn, Jens H; Janosko, Krisztina

    2016-10-03

    Work in a biosafety level 4 (BSL-4) containment laboratory requires time and great attention to detail. The same work that is done in a BSL-2 laboratory with non-high-consequence pathogens will take significantly longer in a BSL-4 setting. This increased time requirement is due to a multitude of factors that are aimed at protecting the researcher from laboratory-acquired infections, the work environment from potential contamination and the local community from possible release of high-consequence pathogens. Inside the laboratory, movement is restricted due to air hoses attached to the mandatory full-body safety suits. In addition, disinfection of every item that is removed from Class II biosafety cabinets (BSCs) is required. Laboratory specialists must be trained in the practices of the BSL-4 laboratory and must show high proficiency in the skills they are performing. The focus of this article is to outline proper procedures and techniques to ensure laboratory biosafety and experimental accuracy using a standard viral plaque assay as an example procedure. In particular, proper techniques to work safely in a BSL-4 environment when performing an experiment will be visually emphasized. These techniques include: setting up a Class II BSC for experiments, proper cleaning of the Class II BSC when finished working, waste management and safe disposal of waste generated inside a BSL-4 laboratory, and the removal of inactivated samples from inside a BSL-4 laboratory to the BSL-2 laboratory.

  13. Biosafety level 3 facility: essential infrastructure in biodefense strategy in the Republic of Croatia

    International Nuclear Information System (INIS)

    Cvetko Krajinovic, L.; Markotic, A.

    2009-01-01

    Wide spectrum of microorganisms nowadays present serious health risks to humans and animals and their potential for use as biological weapons has become an important concern for governments and responsible authorities. This has resulted in the implementation of measures (known as biodefense) directed toward containment of potentially harmful biological agents with the purpose to reduce or eliminate hazards to laboratory workers, other persons, and the outside environment. Many of such pathogens are dangerous pathogens which request biosafety level 3 (BSL-3) facility for research and management. Biosafety level 3 comprises the combinations of standard and special microbiological laboratory practices and techniques, safety equipment, and laboratory facilities recommended for work with indigenous or exotic agents that may cause serious or potentially lethal disease through inhalation route exposure. Croatia is endemic for many of these threatening pathogens/diseases (e.g. tularemia, pulmonary and non-pulmonary tuberculosis, brucellosis, Q fever, glanders, melioidosis, typhoid fever, viral hemorrhagic fevers, hepatitis B and C, HIV etc.). Its strategic geographic position and the overall world rise of international trade and travel unlocks the possibility for importing some new microorganisms or even occurrence of an outbreak of totally unknown infectious origin. We, also, cannot exclude the possibility of the so called deliberately emerging microbes used in intentional bioterrorist purposes. However, it is obvious that Croatia needs infrastructure and well trained human capacities on biosafety level 3 to cope with incoming public health challenges and threats. The fundamental objective of the laboratory under which dangerous agents can safely be handled, is surveillance and quick response, as a key elements in controlling of scenarios referred to above. For that purpose, the first BSL-3 facility in Croatia is in the final phase of its reconstruction at the University

  14. Biosafety level 3 facility: essential infrastructure in biodefense strategy in the Republic of Croatia

    Energy Technology Data Exchange (ETDEWEB)

    Cvetko Krajinovic, L; Markotic, A [University Hospital for Infectious Diseases Dr Fran Mihaljevic, Zagreb (Croatia)

    2009-07-01

    Wide spectrum of microorganisms nowadays present serious health risks to humans and animals and their potential for use as biological weapons has become an important concern for governments and responsible authorities. This has resulted in the implementation of measures (known as biodefense) directed toward containment of potentially harmful biological agents with the purpose to reduce or eliminate hazards to laboratory workers, other persons, and the outside environment. Many of such pathogens are dangerous pathogens which request biosafety level 3 (BSL-3) facility for research and management. Biosafety level 3 comprises the combinations of standard and special microbiological laboratory practices and techniques, safety equipment, and laboratory facilities recommended for work with indigenous or exotic agents that may cause serious or potentially lethal disease through inhalation route exposure. Croatia is endemic for many of these threatening pathogens/diseases (e.g. tularemia, pulmonary and non-pulmonary tuberculosis, brucellosis, Q fever, glanders, melioidosis, typhoid fever, viral hemorrhagic fevers, hepatitis B and C, HIV etc.). Its strategic geographic position and the overall world rise of international trade and travel unlocks the possibility for importing some new microorganisms or even occurrence of an outbreak of totally unknown infectious origin. We, also, cannot exclude the possibility of the so called deliberately emerging microbes used in intentional bioterrorist purposes. However, it is obvious that Croatia needs infrastructure and well trained human capacities on biosafety level 3 to cope with incoming public health challenges and threats. The fundamental objective of the laboratory under which dangerous agents can safely be handled, is surveillance and quick response, as a key elements in controlling of scenarios referred to above. For that purpose, the first BSL-3 facility in Croatia is in the final phase of its reconstruction at the University

  15. Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria

    Directory of Open Access Journals (Sweden)

    Olumuyiwa B. Salu

    2016-10-01

    Full Text Available Introduction: Global travel is an efficient route of transmission for highly infectious pathogens and increases the chances of such pathogens moving from high disease-endemic areas to new regions. We describe the rapid and safe identification of the first imported case of Ebola virus disease in a traveler to Lagos, Nigeria, using conventional reverse transcription polymerase chain reaction (RT-PCR in a biosafety level (BSL-2 facility. Case presentation: On 20 July 2014, a traveler arrived from Liberia at Lagos International Airport and was admitted to a private hospital in Lagos, with clinical suspicion of Ebola virus disease. Methodology and Outcome: Blood and urine specimens were collected, transported to the Virology Unit Laboratory at the College of Medicine, University of Lagos, and processed under stringent biosafety conditions for viral RNA extraction. RT-PCR was set-up to query the Ebola, Lassa and Dengue fever viruses. Amplicons for pan-filoviruses were detected as 300 bp bands on a 1.5% agarose gel image; there were no detectable bands for Lassa and Dengue viral RNA. Nucleotide BLAST and phylogenetic analysis of sequence data of the RNA-dependent RNA polymerase (L gene confirmed the sequence to be Zaire ebolavirus (EBOV/Hsap/ NGA/2014/LIB-NIG 01072014; Genbank: KM251803.1. Conclusion: Our BSL-2 facility in Lagos, Nigeria, was able to safely detect Ebola virus disease using molecular techniques, supporting the reliability of molecular detection of highly infectious viral pathogens under stringent safety guidelines in BSL-2 laboratories. This is a significant lesson for the many under-facilitated laboratories in resource-limited settings, as is predominantly found in sub-Saharan Africa.

  16. Biosafety standards for working with Crimean-Congo hemorrhagic fever virus.

    Science.gov (United States)

    Weidmann, Manfred; Avsic-Zupanc, Tatjana; Bino, Silvia; Bouloy, Michelle; Burt, Felicity; Chinikar, Sadegh; Christova, Iva; Dedushaj, Isuf; El-Sanousi, Ahmed; Elaldi, Nazif; Hewson, Roger; Hufert, Frank T; Humolli, Isme; Jansen van Vuren, Petrus; Koçak Tufan, Zeliha; Korukluoglu, Gülay; Lyssen, Pieter; Mirazimi, Ali; Neyts, Johan; Niedrig, Matthias; Ozkul, Aykut; Papa, Anna; Paweska, Janusz; Sall, Amadou A; Schmaljohn, Connie S; Swanepoel, Robert; Uyar, Yavuz; Weber, Friedemann; Zeller, Herve

    2016-11-01

    In countries from which Crimean-Congo haemorrhagic fever (CCHF) is absent, the causative virus, CCHF virus (CCHFV), is classified as a hazard group 4 agent and handled in containment level (CL)-4. In contrast, most endemic countries out of necessity have had to perform diagnostic tests under biosafety level (BSL)-2 or -3 conditions. In particular, Turkey and several of the Balkan countries have safely processed more than 100 000 samples over many years in BSL-2 laboratories. It is therefore advocated that biosafety requirements for CCHF diagnostic procedures should be revised, to allow the tests required to be performed under enhanced BSL-2 conditions with appropriate biosafety laboratory equipment and personal protective equipment used according to standardized protocols in the countries affected. Downgrading of CCHFV research work from CL-4, BSL-4 to CL-3, BSL-3 should also be considered.

  17. Certification & validation of biosafety level-2 & biosafety level-3 laboratories in Indian settings & common issues

    OpenAIRE

    Devendra T Mourya; Pragya D Yadav; Ajay Khare; Anwar H Khan

    2017-01-01

    With increasing awareness regarding biorisk management worldwide, many biosafety laboratories are being setup in India. It is important for the facility users, project managers and the executing agencies to understand the process of validation and certification of such biosafety laboratories. There are some international guidelines available, but there are no national guidelines or reference standards available in India on certification and validation of biosafety laboratories. There is no ac...

  18. Certification & validation of biosafety level-2 & biosafety level-3 laboratories in Indian settings & common issues.

    Science.gov (United States)

    Mourya, Devendra T; Yadav, Pragya D; Khare, Ajay; Khan, Anwar H

    2017-10-01

    With increasing awareness regarding biorisk management worldwide, many biosafety laboratories are being setup in India. It is important for the facility users, project managers and the executing agencies to understand the process of validation and certification of such biosafety laboratories. There are some international guidelines available, but there are no national guidelines or reference standards available in India on certification and validation of biosafety laboratories. There is no accredited government/private agency available in India to undertake validation and certification of biosafety laboratories. Therefore, the reliance is mostly on indigenous experience, talent and expertise available, which is in short supply. This article elucidates the process of certification and validation of biosafety laboratories in a concise manner for the understanding of the concerned users and suggests the important parameters and criteria that should be considered and addressed during the laboratory certification and validation process.

  19. Certification & validation of biosafety level-2 & biosafety level-3 laboratories in Indian settings & common issues

    Directory of Open Access Journals (Sweden)

    Devendra T Mourya

    2017-01-01

    Full Text Available With increasing awareness regarding biorisk management worldwide, many biosafety laboratories are being setup in India. It is important for the facility users, project managers and the executing agencies to understand the process of validation and certification of such biosafety laboratories. There are some international guidelines available, but there are no national guidelines or reference standards available in India on certification and validation of biosafety laboratories. There is no accredited government/private agency available in India to undertake validation and certification of biosafety laboratories. Therefore, the reliance is mostly on indigenous experience, talent and expertise available, which is in short supply. This article elucidates the process of certification and validation of biosafety laboratories in a concise manner for the understanding of the concerned users and suggests the important parameters and criteria that should be considered and addressed during the laboratory certification and validation process.

  20. Standard practice for cell sorting in a BSL-3 facility.

    Science.gov (United States)

    Perfetto, Stephen P; Ambrozak, David R; Nguyen, Richard; Roederer, Mario; Koup, Richard A; Holmes, Kevin L

    2011-01-01

    Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.

  1. BSL-3 laboratory practices in the United States: comparison of select agent and non-select agent facilities.

    Science.gov (United States)

    Richards, Stephanie L; Pompei, Victoria C; Anderson, Alice

    2014-01-01

    New construction of biosafety level 3 (BSL-3) laboratories in the United States has increased in the past decade to facilitate research on potential bioterrorism agents. The Centers for Disease Control and Prevention inspect BSL-3 facilities and review commissioning documentation, but no single agency has oversight over all BSL-3 facilities. This article explores the extent to which standard operating procedures in US BSL-3 facilities vary between laboratories with select agent or non-select agent status. Comparisons are made for the following variables: personnel training, decontamination, personal protective equipment (PPE), medical surveillance, security access, laboratory structure and maintenance, funding, and pest management. Facilities working with select agents had more complex training programs and decontamination procedures than non-select agent facilities. Personnel working in select agent laboratories were likely to use powered air purifying respirators, while non-select agent laboratories primarily used N95 respirators. More rigorous medical surveillance was carried out in select agent workers (although not required by the select agent program) and a higher level of restrictive access to laboratories was found. Most select agent and non-select agent laboratories reported adequate structural integrity in facilities; however, differences were observed in personnel perception of funding for repairs. Pest management was carried out by select agent personnel more frequently than non-select agent personnel. Our findings support the need to promote high quality biosafety training and standard operating procedures in both select agent and non-select agent laboratories to improve occupational health and safety.

  2. The Main Biological Hazards in Animal Biosafety Level 2 Facilities and Strategies for Control.

    Science.gov (United States)

    Li, Xiao Yan; Xue, Kang Ning; Jiang, Jin Sheng; Lu, Xuan Cheng

    2016-04-01

    Concern about the biological hazards involved in microbiological research, especially research involving laboratory animals, has increased in recent years. Working in an animal biosafety level 2 facility (ABSL-2), commonly used for research on infectious diseases, poses various biological hazards. Here, the regulations and standards related to laboratory biosafety in China are introduced, the potential biological hazards present in ABSL-2 facilities are analyzed, and a series of strategies to control the hazards are presented. Copyright © 2016 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

  3. Mobile/Modular BSL-4 Facilities for Meeting Restricted Earth Return Containment Requirements

    Science.gov (United States)

    Calaway, M. J.; McCubbin, F. M.; Allton, J. H.; Zeigler, R. A.; Pace, L. F.

    2017-01-01

    NASA robotic sample return missions designated Category V Restricted Earth Return by the NASA Planetary Protection Office require sample containment and biohazard testing in a receiving laboratory as directed by NASA Procedural Requirement (NPR) 8020.12D - ensuring the preservation and protection of Earth and the sample. Currently, NPR 8020.12D classifies Restricted Earth Return for robotic sample return missions from Mars, Europa, and Enceladus with the caveat that future proposed mission locations could be added or restrictions lifted on a case by case basis as scientific knowledge and understanding of biohazards progresses. Since the 1960s, sample containment from an unknown extraterrestrial biohazard have been related to the highest containment standards and protocols known to modern science. Today, Biosafety Level (BSL) 4 standards and protocols are used to study the most dangerous high-risk diseases and unknown biological agents on Earth. Over 30 BSL-4 facilities have been constructed worldwide with 12 residing in the United States; of theses, 8 are operational. In the last two decades, these brick and mortar facilities have cost in the hundreds of millions of dollars dependent on the facility requirements and size. Previous mission concept studies for constructing a NASA sample receiving facility with an integrated BSL-4 quarantine and biohazard testing facility have also been estimated in the hundreds of millions of dollars. As an alternative option, we have recently conducted an initial trade study for constructing a mobile and/or modular sample containment laboratory that would meet all BSL-4 and planetary protection standards and protocols at a faction of the cost. Mobile and modular BSL-2 and 3 facilities have been successfully constructed and deployed world-wide for government testing of pathogens and pharmaceutical production. Our study showed that a modular BSL-4 construction could result in approximately 90% cost reduction when compared to

  4. The European network of Biosafety-Level-4 laboratories: enhancing European preparedness for new health threats.

    Science.gov (United States)

    Nisii, C; Castilletti, C; Di Caro, A; Capobianchi, M R; Brown, D; Lloyd, G; Gunther, S; Lundkvist, A; Pletschette, M; Ippolito, G

    2009-08-01

    Emerging and re-emerging infections and possible bioterrorism acts will continue to challenge both the medical community and civilian populations worldwide, urging health authorities to respond rapidly and effectively. Established in 2005, the European Community (EC)-funded European Network of Biosafety-Level-4 laboratories (Euronet-P4), which brings together the laboratories in Porton Down, London, Hamburg, Marburg, Solna, Lyon and Rome, seeks to increase international collaboration in the areas of high containment laboratory biosafety and viral diagnostic capability, to strengthen Europe's capacity to respond to an infectious disease emergency, and to offer assistance to countries not equipped with such costly facilities. Network partners have agreed on a common strategy to fill the gaps identified in the field of risk group-4 agents' laboratory diagnosis, namely the lack of standardization and of reference samples. The network has received a further 3-year funding, to offer assistance to external laboratories, and to start the planning of field activities.

  5. Biosafety and Biosecurity in European Containment Level 3 Laboratories: Focus on French Recent Progress and Essential Requirements

    Directory of Open Access Journals (Sweden)

    Boris Pastorino

    2017-05-01

    Full Text Available Even if European Union (EU Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work, national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a relatively new concept and a few EU Member States are known to have introduced national laboratory biosecurity legislation. In France, recent regulations have reinforced biosafety/biosecurity in containment level 3 (CL-3 laboratories but they concern a specific list of pathogens with no correlation in other European Members States. The objective of this review was to summarize European biosafety/biosecurity measures concerning CL-3 facilities focusing on French specificities. Essential requirements needed to preserve efficient biosafety measures when manipulating risk group 3 biological agents are highlighted. In addition, International, European and French standards related to containment laboratory planning, operation or biosafety equipment are described to clarify optimal biosafety and biosecurity requirements.

  6. Biosafety and Biosecurity in European Containment Level 3 Laboratories: Focus on French Recent Progress and Essential Requirements.

    Science.gov (United States)

    Pastorino, Boris; de Lamballerie, Xavier; Charrel, Rémi

    2017-01-01

    Even if European Union (EU) Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work , national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a relatively new concept and a few EU Member States are known to have introduced national laboratory biosecurity legislation. In France, recent regulations have reinforced biosafety/biosecurity in containment level 3 (CL-3) laboratories but they concern a specific list of pathogens with no correlation in other European Members States. The objective of this review was to summarize European biosafety/biosecurity measures concerning CL-3 facilities focusing on French specificities. Essential requirements needed to preserve efficient biosafety measures when manipulating risk group 3 biological agents are highlighted. In addition, International, European and French standards related to containment laboratory planning, operation or biosafety equipment are described to clarify optimal biosafety and biosecurity requirements.

  7. Biosafety Manual

    Energy Technology Data Exchange (ETDEWEB)

    King, Bruce W.

    2010-05-18

    Work with or potential exposure to biological materials in the course of performing research or other work activities at Lawrence Berkeley National Laboratory (LBNL) must be conducted in a safe, ethical, environmentally sound, and compliant manner. Work must be conducted in accordance with established biosafety standards, the principles and functions of Integrated Safety Management (ISM), this Biosafety Manual, Chapter 26 (Biosafety) of the Health and Safety Manual (PUB-3000), and applicable standards and LBNL policies. The purpose of the Biosafety Program is to protect workers, the public, agriculture, and the environment from exposure to biological agents or materials that may cause disease or other detrimental effects in humans, animals, or plants. This manual provides workers; line management; Environment, Health, and Safety (EH&S) Division staff; Institutional Biosafety Committee (IBC) members; and others with a comprehensive overview of biosafety principles, requirements from biosafety standards, and measures needed to control biological risks in work activities and facilities at LBNL.

  8. Practical biosafety in the tuberculosis laboratory: containment at the source is what truly counts.

    Science.gov (United States)

    van Soolingen, D; Wisselink, H J; Lumb, R; Anthony, R; van der Zanden, A; Gilpin, C

    2014-08-01

    In industrialised countries, sufficient resources for establishing and maintaining fully equipped biosafety level 3 (BSL-3) laboratories according to international standards are generally available. BSL-3 laboratories are designed to provide several layers of containment to protect the laboratory worker as well as the outside environment and community from risk of exposure in case of local contamination. However, such facilities are scarce in high-burden settings, primarily due to the high financial burden and complexity of the initial construction and/or regular maintenance. Measures to prevent unintended exposure to Mycobacterium tuberculosis during laboratory manipulation of specimens and cultures is the first, and by far the most important, aspect of containment. This paper focuses on the need for risk containment at source. Assuming that in many settings the establishment of BSL-3 laboratories with all the required features is not achievable, this paper also discusses the minimum requirements necessary to mitigate risks associated with particular laboratory procedures. The term 'TB containment laboratory' is used throughout this paper to describe the minimum requirements for a laboratory suitable for high-risk procedures. The TB containment laboratory has many, but not all, of the features of a BSL-3 laboratory.

  9. Biosafety Education and Training Programs for Ukrainian Microbiologists

    International Nuclear Information System (INIS)

    Pushkina, V.; Volyansky, A.; Popova, N.

    2007-01-01

    In the period of the Soviet Union Ukrainian Mechnikov Anti-Plaque Research Institute was one of the main bases of centralized training for laboratory diagnosis of especially dangerous infections. Not only specialists, but medical technicians were obligatory trained. In training programs special attention was paid to the safety regime in accurate work out of practical manipulations in investigational classical methods (cultivating technique, pipeting, animals' infection and dissection, etc.), protective clothes usage, anti-epidemic measures use at different accidents. This approach gave effective results not only in laboratories but also during field work (natural plaque foci investigations, etc.) and at emergencies. Recently in world practice to increase the level of biosafety technical equipment and devices are developed and used very intensively. During training maximal time is paid to their mastering. At such training biosafety practically depends on safe and reliable work of engineer-technical systems. At present in Ukrainian Anti-Plaque Institute with the support of Canadian Government Training Centre on biosafety and biodefense for specialists of Ukraine and FSU countries is being organized. Teaching programs will include complex study of hand manipulations and modern technical means knowledge. To our mind such initial training had to be available for all specialists of BSL 1-2 microbiological laboratories of any subordination. For this goal all kinds of programs will be developed. Such complex approach will promote to decrease biological risks in microbiological laboratories and prevent infectious agents import from working territories.(author)

  10. Triage and management of accidental laboratory exposures to biosafety level-3 and -4 agents.

    Science.gov (United States)

    Jahrling, Peter; Rodak, Colleen; Bray, Mike; Davey, Richard T

    2009-06-01

    The recent expansion of biocontainment laboratory capacity in the United States has drawn attention to the possibility of occupational exposures to BSL-3 and -4 agents and has prompted a reassessment of medical management procedures and facilities to deal with these contingencies. A workshop hosted by the National Interagency Biodefense Campus was held in October 2007 and was attended by representatives of all existing and planned BSL-4 research facilities in the U.S. and Canada. This report summarizes important points of discussion and recommendations for future coordinated action, including guidelines for the engineering and operational controls appropriate for a hospital care and isolation unit. Recommendations pertained to initial management of exposures (ie, immediate treatment of penetrating injuries, reporting of exposures, initial evaluation, and triage). Isolation and medical care in a referral hospital (including minimum standards for isolation units), staff recruitment and training, and community outreach also were addressed. Workshop participants agreed that any unit designated for the isolation and treatment of laboratory employees accidentally infected with a BSL-3 or -4 pathogen should be designed to maximize the efficacy of patient care while minimizing the risk of transmission of infection. Further, participants concurred that there is no medically based rationale for building care and isolation units to standards approximating a BSL-4 laboratory. Instead, laboratory workers accidentally exposed to pathogens should be cared for in hospital isolation suites staffed by highly trained professionals following strict infection control procedures.

  11. Handling small arbovirus vectors safely during biosafety level 3 containment: Culicoides variipennis sonorensis (Diptera:Ceratopogonidae) and exotic bluetongue viruses.

    Science.gov (United States)

    Hunt, G J; Tabachnick, W J

    1996-05-01

    Equipment and procedures are described for biosafety level 3 (BL-3) containment work with small, zoophilic arthropods. BL-3 classified pathogens always must be manipulated in biological safety cabinets. Procedures, including physical barriers and handling methods, that prevent the escape of potentially virus-infected insects are discussed, and the use of a monitoring system for insect security is explained. The inability to recover escaped minute, flying insects poses a major difference from similar work with larger insects, such as mosquitoes. Methods were developed for the safe and secure handling of Culicoides variipennis sonorensis Wirth & Jones infected with exotic bluetongue viruses during BL-3 containment.

  12. Non-Invasive Optical Sensor Based Approaches for Monitoring Virus Culture to Minimize BSL3 Laboratory Entry

    Directory of Open Access Journals (Sweden)

    Viswanath Ragupathy

    2015-06-01

    Full Text Available High titers of infectious viruses for vaccine and diagnostic reference panel development are made by infecting susceptible mammalian cells. Laboratory procedures are strictly performed in a Bio-Safety Level-3 (BSL3 laboratory and each entry and exit involves the use of  disposable Personnel Protective Equipment (PPE to observe cell culture conditions. Routine PPE use involves significant recurring costs. Alternative non-invasive optical sensor based approaches to remotely monitor cell culture may provide a promising and cost effective approach to monitor infectious virus cultures resulting in lower disruption and costs. We report here the monitoring of high titer cultures of Human Immunodeficiency Virus-1 (HIV-1 and Herpes Simplex Virus-2 (HSV-2 remotely with the use of optical oxygen sensors aseptically placed inside the cell culture vessel. The replacement of culture media for cell and virus propagation and virus load monitoring was effectively performed using this fluorescent sensor and resulted in half the number of visits to the BSL3 lab (five versus ten.

  13. Feasibility of establishing a biosafety level 3 tuberculosis culture laboratory of acceptable quality standards in a resource-limited setting: an experience from Uganda.

    Science.gov (United States)

    Ssengooba, Willy; Gelderbloem, Sebastian J; Mboowa, Gerald; Wajja, Anne; Namaganda, Carolyn; Musoke, Philippa; Mayanja-Kizza, Harriet; Joloba, Moses Lutaakome

    2015-01-15

    Despite the recent innovations in tuberculosis (TB) and multi-drug resistant TB (MDR-TB) diagnosis, culture remains vital for difficult-to-diagnose patients, baseline and end-point determination for novel vaccines and drug trials. Herein, we share our experience of establishing a BSL-3 culture facility in Uganda as well as 3-years performance indicators and post-TB vaccine trials (pioneer) and funding experience of sustaining such a facility. Between September 2008 and April 2009, the laboratory was set-up with financial support from external partners. After an initial procedure validation phase in parallel with the National TB Reference Laboratory (NTRL) and legal approvals, the laboratory registered for external quality assessment (EQA) from the NTRL, WHO, National Health Laboratories Services (NHLS), and the College of American Pathologists (CAP). The laboratory also instituted a functional quality management system (QMS). Pioneer funding ended in 2012 and the laboratory remained in self-sustainability mode. The laboratory achieved internationally acceptable standards in both structural and biosafety requirements. Of the 14 patient samples analyzed in the procedural validation phase, agreement for all tests with NTRL was 90% (P 80% in all years from NTRL, CAP, and NHLS, and culture was 100% for CAP panels and above regional average scores for all years with NHLS. Quarterly DST scores from WHO-EQA ranged from 78% to 100% in 2010, 80% to 100% in 2011, and 90 to 100% in 2012. From our experience, it is feasible to set-up a BSL-3 TB culture laboratory with acceptable quality performance standards in resource-limited countries. With the demonstrated quality of work, the laboratory attracted more research groups and post-pioneer funding, which helped to ensure sustainability. The high skilled experts in this research laboratory also continue to provide an excellent resource for the needed national discussion of the laboratory and quality management systems.

  14. Genome-wide siRNA Screening at Biosafety Level 4 Reveals a Crucial Role for Fibrillarin in Henipavirus Infection.

    Directory of Open Access Journals (Sweden)

    Celine Deffrasnes

    2016-03-01

    Full Text Available Hendra and Nipah viruses (genus Henipavirus, family Paramyxoviridae are highly pathogenic bat-borne viruses. The need for high biocontainment when studying henipaviruses has hindered the development of therapeutics and knowledge of the viral infection cycle. We have performed a genome-wide siRNA screen at biosafety level 4 that identified 585 human proteins required for henipavirus infection. The host protein with the largest impact was fibrillarin, a nucleolar methyltransferase that was also required by measles, mumps and respiratory syncytial viruses for infection. While not required for cell entry, henipavirus RNA and protein syntheses were greatly impaired in cells lacking fibrillarin, indicating a crucial role in the RNA replication phase of infection. During infection, the Hendra virus matrix protein co-localized with fibrillarin in cell nucleoli, and co-associated as a complex in pulldown studies, while its nuclear import was unaffected in fibrillarin-depleted cells. Mutagenesis studies showed that the methyltransferase activity of fibrillarin was required for henipavirus infection, suggesting that this enzyme could be targeted therapeutically to combat henipavirus infections.

  15. Biosafety and Biosecurity in European Containment Level 3 Laboratories: Focus on French Recent Progress and Essential Requirements

    OpenAIRE

    Boris Pastorino; Boris Pastorino; Xavier de Lamballerie; Xavier de Lamballerie; Rémi Charrel; Rémi Charrel

    2017-01-01

    Even if European Union (EU) Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work, national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a r...

  16. Biosafety in the Laboratory: Prudent Practices for the Handling and Disposal of Infectious Materials

    Science.gov (United States)

    1989-03-01

    psittacosis, lymphogranuloma animal disease diagnostic laboratory). Biosafety Level venereum (LGV), and trachoma are documented APPENDIX A I11 hazards and...127 see also Facilities Lymphogranuloma venereum , 110-111 Laboratory practices academic laboratories, 68-69 M Biosafety Level 1, 90 Biosafety Level

  17. Good laboratory practices guarantee biosafety in the Sierra Leone-China friendship biosafety laboratory

    OpenAIRE

    Wang, Qin; Zhou, Wei-Min; Zhang, Yong; Wang, Huan-Yu; Du, Hai-Jun; Nie, Kai; Song, Jing-Dong; Xiao, Kang; Lei, Wen-Wen; Guo, Jian-Qiang; Wei, He-Jiang; Cai, Kun; Wang, Yan-Hai; Wu, Jiang; Kamara, Gerard

    2016-01-01

    Background The outbreak of Ebola virus disease (EVD) in West Africa between 2014 and 2015 was the largest EDV epidemic since the identification of Ebola virus (EBOV) in 1976, and the countries most strongly affected were Sierra Leone, Guinea, and Liberia. Findings The Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab), a fixed Biosafety Level 3 laboratory in the capital city of Sierra Leone, was established by the Chinese government and has been active in EBOV ...

  18. Integration of Biosafety into Core Facility Management

    Science.gov (United States)

    Fontes, Benjamin

    2013-01-01

    This presentation will discuss the implementation of biosafety policies for small, medium and large core laboratories with primary shared objectives of ensuring the control of biohazards to protect core facility operators and assure conformity with applicable state and federal policies, standards and guidelines. Of paramount importance is the educational process to inform core laboratories of biosafety principles and policies and to illustrate the technology and process pathways of the core laboratory for biosafety professionals. Elevating awareness of biohazards and the biosafety regulatory landscape among core facility operators is essential for the establishment of a framework for both project and material risk assessment. The goal of the biohazard risk assessment process is to identify the biohazard risk management parameters to conduct the procedure safely and in compliance with applicable regulations. An evaluation of the containment, protective equipment and work practices for the procedure for the level of risk identified is facilitated by the establishment of a core facility registration form for work with biohazards and other biological materials with potential risk. The final step in the biocontainment process is the assumption of Principal Investigator role with full responsibility for the structure of the site-specific biosafety program plan by core facility leadership. The presentation will provide example biohazard protocol reviews and accompanying containment measures for core laboratories at Yale University.

  19. Segmentation of British Sign Language (BSL): Mind the gap!

    OpenAIRE

    Orfanidou, E.; McQueen, J.; Adam, R.; Morgan, G.

    2015-01-01

    This study asks how users of British Sign Language (BSL) recognize individual signs in connected sign sequences. We examined whether this is achieved through modality-specific or modality-general segmentation procedures. A modality-specific feature of signed languages is that, during continuous signing, there are salient transitions between sign locations. We used the sign-spotting task to ask if and how BSL signers use these transitions in segmentation. A total of 96 real BSL signs were prec...

  20. Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

    Directory of Open Access Journals (Sweden)

    Michael B. Sherman

    2015-05-01

    Full Text Available A unique biological safety level (BSL-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2 decontamination cycles.

  1. Laboratory biorisk management biosafety and biosecurity

    CERN Document Server

    Salerno, Reynolds M

    2015-01-01

    Over the past two decades bioscience facilities worldwide have experienced multiple safety and security incidents, including many notable incidents at so-called ""sophisticated facilities"" in North America and Western Europe. This demonstrates that a system based solely on biosafety levels and security regulations may not be sufficient.Setting the stage for a substantively different approach for managing the risks of working with biological agents in laboratories, Laboratory Biorisk Management: Biosafety and Biosecurity introduces the concept of biorisk management-a new paradigm that encompas

  2. Biosafety Risk Assessment Methodology

    Energy Technology Data Exchange (ETDEWEB)

    Caskey, Susan Adele [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). International Biological Threat Reduction Program; Gaudioso, Jennifer M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). International Biological Threat Reduction Program; Salerno, Reynolds Mathewson [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). International Biological Threat Reduction Program; Wagner, Stefan M. [Public Health Agency of Canada, Winnipeg, MB (Canada). Canadian Science Centre for Human and Animal Health (CSCHAH); Shigematsu, Mika [National Inst. of Infectious Diseases (NIID), Tokyo (Japan); Risi, George [Infectious Disease Specialists, P.C, Missoula, MT (United States); Kozlovac, Joe [US Dept. of Agriculture (USDA)., Beltsville, MD (United States); Halkjaer-Knudsen, Vibeke [Statens Serum Inst., Copenhagen (Denmark); Prat, Esmeralda [Bayer CropScience, Monheim am Rhein (Germany)

    2010-10-01

    Laboratories that work with biological agents need to manage their safety risks to persons working the laboratories and the human and animal community in the surrounding areas. Biosafety guidance defines a wide variety of biosafety risk mitigation measures, which include measures which fall under the following categories: engineering controls, procedural and administrative controls, and the use of personal protective equipment; the determination of which mitigation measures should be used to address the specific laboratory risks are dependent upon a risk assessment. Ideally, a risk assessment should be conducted in a manner which is standardized and systematic which allows it to be repeatable and comparable. A risk assessment should clearly define the risk being assessed and avoid over complication.

  3. Laboratory biosafety manual

    Energy Technology Data Exchange (ETDEWEB)

    1983-01-01

    This book is in three sections; basic standards of laboratory design and equipment; procedures for safe laboratory practice; and the selection and use of essential biosafety equipment. The intention is that the guidance given in the book should have a broad basis and international application, and that it should be a source from which manuals applicable to local and special conditions can be usefully derived.

  4. Feasibility of establishing a biosafety level 3 tuberculosis culture laboratory of acceptable quality standards in a resource-limited setting: an experience from Uganda

    NARCIS (Netherlands)

    Ssengooba, Willy; Gelderbloem, Sebastian J.; Mboowa, Gerald; Wajja, Anne; Namaganda, Carolyn; Musoke, Philippa; Mayanja-Kizza, Harriet; Joloba, Moses Lutaakome

    2015-01-01

    Background: Despite the recent innovations in tuberculosis (TB) and multi-drug resistant TB (MDR-TB) diagnosis, culture remains vital for difficult-to-diagnose patients, baseline and end-point determination for novel vaccines and drug trials. Herein, we share our experience of establishing a BSL-3

  5. Good laboratory practices guarantee biosafety in the Sierra Leone-China friendship biosafety laboratory.

    Science.gov (United States)

    Wang, Qin; Zhou, Wei-Min; Zhang, Yong; Wang, Huan-Yu; Du, Hai-Jun; Nie, Kai; Song, Jing-Dong; Xiao, Kang; Lei, Wen-Wen; Guo, Jian-Qiang; Wei, He-Jiang; Cai, Kun; Wang, Yan-Hai; Wu, Jiang; Kamara, Gerard; Kamara, Idrissa; Wei, Qiang; Liang, Mi-Fang; Wu, Gui-Zhen; Dong, Xiao-Ping

    2016-06-23

    The outbreak of Ebola virus disease (EVD) in West Africa between 2014 and 2015 was the largest EDV epidemic since the identification of Ebola virus (EBOV) in 1976, and the countries most strongly affected were Sierra Leone, Guinea, and Liberia. The Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab), a fixed Biosafety Level 3 laboratory in the capital city of Sierra Leone, was established by the Chinese government and has been active in EBOV detection since 11 March 2015. Complete management and program documents were created for the SLE-CHN Biosafety Lab, and it was divided into four zones (the green, yellow, brown, and red zones) based on the risk assessment. Different types of safe and appropriate personnel protection equipment (PPE) are used in different zones of the laboratory, and it fully meets the Biosafety Level 3 laboratory standards of the World Health Organization. Good preparedness, comprehensive risk assessment and operation documents, appropriate PPE, effective monitoring and intensive training, together with well-designed and reasonable laboratory sectioning are essential for guaranteeing biosafety.

  6. Thermal inactivation of avian viral and bacterial pathogens in an effluent treatment system within a biosafety level 2 and 3 enhanced facility

    Science.gov (United States)

    Avian influenza (AI) virus, avian paramyxovirus Type 1 (APMV-1 or Newcastle disease virus [NDV]), reovirus, rotavirus, turkey astrovirus (TAstV), avian metapneumovirus (aMPV), Marek’s disease virus (MDV-1), avian parvovirus (ChPV) and Salmonella enterica serovar Enteritidis are significant biosafety...

  7. Developing biosafety risk hypotheses for invertebrates exposed to GM plants using conceptual food webs: a case study with elevated triacylglyceride levels in ryegrass.

    Science.gov (United States)

    Barratt, Barbara I P; Todd, Jacqui H; Burgess, Elisabeth P J; Malone, Louise A

    2010-01-01

    Regulators are acutely aware of the need for meaningful risk assessments to support decisions on the safety of GM crops to non-target invertebrates in determining their suitability for field release. We describe a process for developing appropriate, testable risk hypotheses for invertebrates in agroecosystems that might be exposed to plants developed by GM and future novel technologies. An existing model (PRONTI) generates a ranked list of invertebrate species for biosafety testing by accessing a database of biological, ecological and food web information about species which occur in cropping environments and their potential interactions with a particular stressor (Eco Invertebase). Our objective in this contribution is to explore and further utilise these resources to assist in the process of problem formulation by identifying potentially significant effects of the stressor on the invertebrate community and the ecosystem services they provide. We propose that for high ranking species, a conceptual food web using information in Eco Invertebase is constructed, and using an accepted regulatory risk analysis framework, the likelihood of risk, and magnitude of impact for each link in the food web is evaluated. Using as filters only those risks evaluated as likely to extremely likely, and the magnitude of an effect being considered as moderate to massive, the most significant potential effects can be identified. A stepwise approach is suggested to develop a sequence of appropriate tests. The GM ryegrass plant used as the "stressor" in this study has been modified to increase triacylglyceride levels in foliage by 100% to increase the metabolisable energy content of forage for grazing animals. The high-ranking "test" species chosen to illustrate the concept are New Zealand native species Wiseana cervinata (Walker) (Lepidoptera: Hepialidae), Persectania aversa (Walker) (Lepidoptera: Noctuidae), and the self-introduced grey field slug, Deroceras reticulatum (Müller).

  8. Evaluation of LLNL BSL-3 Maximum Credible Event Potential Consequence to the General Population and Surrounding Environment

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2010-08-16

    The purpose of this evaluation is to establish reproducibility of the analysis and consequence results to the general population and surrounding environment in the LLNL Biosafety Level 3 Facility Environmental Assessment (LLNL 2008).

  9. Segmentation of British Sign Language (BSL): Mind the gap!

    NARCIS (Netherlands)

    Orfanidou, E.; McQueen, J.M.; Adam, R.; Morgan, G.

    2015-01-01

    This study asks how users of British Sign Language (BSL) recognize individual signs in connected sign sequences. We examined whether this is achieved through modality-specific or modality-general segmentation procedures. A modality-specific feature of signed languages is that, during continuous

  10. Sociolinguistic Variation in the Nativisation of BSL Fingerspelling

    Directory of Open Access Journals (Sweden)

    Brown Matt

    2017-01-01

    Full Text Available British Sign Language (BSL is a visual-gestural language distinct from spoken languages used in the United Kingdom but in contact with them. One product of this contact is the use of fingerspelling to represent English words via their orthography. Fingerspelled loans can become “nativised”, adapting manual production to conform more closely to the native lexicon’s inventory of phonemic constraints. Much of the previous literature on fingerspelling has focused on one-handed systems but, unlike the majority of sign languages, BSL uses a two-handed manual alphabet. What is the nature of nativisation in BSL, and does it exhibit sociolinguistic variation? We apply a cross-linguistic model of nativisation to BSL Corpus conversation and narrative data (http://bslcorpusproject.org obtained from 150 signers in 6 UK regions. Mixed effects modelling is employed to determine the influence of social factors. Results show that the participants’ home region is the most significant factor, with London and Birmingham signers significantly favouring use of fully nativised fingerspelled forms. Non-nativised sequences are significantly favoured in signers of increasing age in Glasgow and Belfast. Gender and parental language background are not found to be significant factors in nativisation. The findings also suggest a form of reduction specific to London and Birmingham.

  11. Segmentation of British Sign Language (BSL): mind the gap!

    Science.gov (United States)

    Orfanidou, Eleni; McQueen, James M; Adam, Robert; Morgan, Gary

    2015-01-01

    This study asks how users of British Sign Language (BSL) recognize individual signs in connected sign sequences. We examined whether this is achieved through modality-specific or modality-general segmentation procedures. A modality-specific feature of signed languages is that, during continuous signing, there are salient transitions between sign locations. We used the sign-spotting task to ask if and how BSL signers use these transitions in segmentation. A total of 96 real BSL signs were preceded by nonsense signs which were produced in either the target location or another location (with a small or large transition). Half of the transitions were within the same major body area (e.g., head) and half were across body areas (e.g., chest to hand). Deaf adult BSL users (a group of natives and early learners, and a group of late learners) spotted target signs best when there was a minimal transition and worst when there was a large transition. When location changes were present, both groups performed better when transitions were to a different body area than when they were within the same area. These findings suggest that transitions do not provide explicit sign-boundary cues in a modality-specific fashion. Instead, we argue that smaller transitions help recognition in a modality-general way by limiting lexical search to signs within location neighbourhoods, and that transitions across body areas also aid segmentation in a modality-general way, by providing a phonotactic cue to a sign boundary. We propose that sign segmentation is based on modality-general procedures which are core language-processing mechanisms.

  12. Integration of Biosafety into Core Facility Management

    OpenAIRE

    Fontes, Benjamin

    2013-01-01

    This presentation will discuss the implementation of biosafety policies for small, medium and large core laboratories with primary shared objectives of ensuring the control of biohazards to protect core facility operators and assure conformity with applicable state and federal policies, standards and guidelines. Of paramount importance is the educational process to inform core laboratories of biosafety principles and policies and to illustrate the technology and process pathways of the core l...

  13. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology.

    Science.gov (United States)

    Bohannon, J Kyle; Janosko, Krisztina; Holbrook, Michael R; Barr, Jason; Pusl, Daniela; Bollinger, Laura; Coe, Linda; Hensley, Lisa E; Jahrling, Peter B; Wada, Jiro; Kuhn, Jens H; Lackemeyer, Matthew G

    2016-10-03

    Aerosol or inhalational studies of high-consequence pathogens have recently been increasing in number due to the perceived threat of intentional aerosol releases or unexpected natural aerosol transmission. Specific laboratories designed to perform these experiments require tremendous engineering controls to provide a safe and secure working environment and constant systems maintenance to sustain functionality. Class III biosafety cabinets, also referred to as gloveboxes, are gas-tight enclosures with non-opening windows. These cabinets are maintained under negative pressure by double high-efficiency-particulate-air (HEPA)-filtered exhaust systems and are the ideal primary containment for housing aerosolization equipment. A well planned workflow between staff members within high containment from, for instance, an animal biosafety level-4 (ABSL-4) suit laboratory to the ABSL-4 cabinet laboratory is a crucial component for successful experimentation. For smooth study execution, establishing a communication network, moving equipment and subjects, and setting up and placing equipment, requires staff members to meticulously plan procedures prior to study initiation. Here, we provide an overview and a visual representation of how aerobiology research is conducted at the National Institutes of Health, National Institute of Allergy and Infectious Diseases Integrated Research Facility at Fort Detrick, Maryland, USA, within an ABSL-4 environment.

  14. Prospecting for Microelement Function and Biosafety Assessment of Transgenic Cereal Plants

    Directory of Open Access Journals (Sweden)

    Xiaofen Yu

    2018-03-01

    Full Text Available Microelement contents and metabolism are vitally important for cereal plant growth and development as well as end-use properties. While minerals phytotoxicity harms plants, microelement deficiency also affects human health. Genetic engineering provides a promising way to solve these problems. As plants vary in abilities to uptake, transport, and accumulate minerals, and the key enzymes acting on that process is primarily presented in this review. Subsequently, microelement function and biosafety assessment of transgenic cereal plants have become a key issue to be addressed. Progress in genetic engineering of cereal plants has been made with the introduction of quality, high-yield, and resistant genes since the first transgenic rice, corn, and wheat were born in 1988, 1990, and 1992, respectively. As the biosafety issue of transgenic cereal plants has now risen to be a top concern, many studies on transgenic biosafety have been carried out. Transgenic cereal biosafety issues mainly include two subjects, environmental friendliness and end-use safety. Different levels of gene confirmation, genomics, proteomics, metabolomics and nutritiomics, absorption, metabolism, and function have been investigated. Also, the different levels of microelement contents have been measured in transgenic plants. Based on the motivation of the requested biosafety, systematic designs, and analysis of transgenic cereal are also presented in this review paper.

  15. Prospecting for Microelement Function and Biosafety Assessment of Transgenic Cereal Plants.

    Science.gov (United States)

    Yu, Xiaofen; Luo, Qingchen; Huang, Kaixun; Yang, Guangxiao; He, Guangyuan

    2018-01-01

    Microelement contents and metabolism are vitally important for cereal plant growth and development as well as end-use properties. While minerals phytotoxicity harms plants, microelement deficiency also affects human health. Genetic engineering provides a promising way to solve these problems. As plants vary in abilities to uptake, transport, and accumulate minerals, and the key enzymes acting on that process is primarily presented in this review. Subsequently, microelement function and biosafety assessment of transgenic cereal plants have become a key issue to be addressed. Progress in genetic engineering of cereal plants has been made with the introduction of quality, high-yield, and resistant genes since the first transgenic rice, corn, and wheat were born in 1988, 1990, and 1992, respectively. As the biosafety issue of transgenic cereal plants has now risen to be a top concern, many studies on transgenic biosafety have been carried out. Transgenic cereal biosafety issues mainly include two subjects, environmental friendliness and end-use safety. Different levels of gene confirmation, genomics, proteomics, metabolomics and nutritiomics, absorption, metabolism, and function have been investigated. Also, the different levels of microelement contents have been measured in transgenic plants. Based on the motivation of the requested biosafety, systematic designs, and analysis of transgenic cereal are also presented in this review paper.

  16. Genetically modified crops: detection strategies and biosafety issues.

    Science.gov (United States)

    Kamle, Suchitra; Ali, Sher

    2013-06-15

    Genetically modified (GM) crops are increasingly gaining acceptance but concurrently consumers' concerns are also increasing. The introduction of Bacillus thuringiensis (Bt) genes into the plants has raised issues related to its risk assessment and biosafety. The International Regulations and the Codex guidelines regulate the biosafety requirements of the GM crops. In addition, these bodies synergize and harmonize the ethical issues related to the release and use of GM products. The labeling of GM crops and their products are mandatory if the genetically modified organism (GMO) content exceeds the levels of a recommended threshold. The new and upcoming GM crops carrying multiple stacked traits likely to be commercialized soon warrant sensitive detection methods both at the DNA and protein levels. Therefore, traceability of the transgene and its protein expression in GM crops is an important issue that needs to be addressed on a priority basis. The advancement in the area of molecular biology has made available several bioanalytical options for the detection of GM crops based on DNA and protein markers. Since the insertion of a gene into the host genome may even cause copy number variation, this may be uncovered using real time PCR. Besides, assessing the exact number of mRNA transcripts of a gene, correlation between the template activity and expressed protein may be established. Here, we present an overview on the production of GM crops, their acceptabilities, detection strategies, biosafety issues and potential impact on society. Further, overall future prospects are also highlighted. Copyright © 2013 Elsevier B.V. All rights reserved.

  17. Progress and Challenges for Implementation of the Common Market for Eastern and Southern Africa Policy on Biotechnology and Biosafety

    Science.gov (United States)

    Waithaka, Michael; Belay, Getachew; Kyotalimye, Miriam; Karembu, Margaret

    2015-01-01

    In 2001, the Meeting of the COMESA Ministers of Agriculture raised concerns that proliferation of genetically modified organisms (GMOs) could impact significantly on trade and food security in the region. This triggered studies on a regional approach to biotechnology and biosafety policy in Eastern and Southern Africa. The studies and stakeholder consultations revealed that farm incomes would increase if they switched from conventional varieties of cotton and maize to genetically modified (GM) counterparts. Commercial risks associated with exports to GM sensitive destinations, e.g., EU were negligible. Intra-regional trade would be affected since exports of GM sensitive commodities, such as maize, cotton, and soya bean, mainly go to other African countries. These findings justified the need to consider a regional approach to biosafety and led to the drafting of a regional policy in 2009. The draft policies were discussed in regional and national workshops between 2010 and 2012 for wider ownership. The workshops involved key stakeholders including ministries of agriculture, trade, environment, national biosafety focal points, biosafety competent authorities, academia, seed traders, millers, the media, food relief agencies, the industry, civil society, competent authorities, and political opinion leaders. The COMESA Council of Ministers in February 2014 adopted the COMESA policy on biotechnology and biosafety that takes into account the sovereign right of each member state. Key provisions of the policy include recognition of the benefits and risks associated with GMOs; establishment of a regional-level biosafety risk-assessment system; national-level final decision, and capacity building assistance to member states. The policies are the first regional effort in Africa to develop a coordinated mechanism for handling biosafety issues related to GMO use. A regional approach to biotechnology and biosafety is expected to foster inter-country cooperation through the

  18. Progress and Challenges for Implementation of the Common Market for Eastern and Southern Africa Policy on Biotechnology and Biosafety.

    Science.gov (United States)

    Waithaka, Michael; Belay, Getachew; Kyotalimye, Miriam; Karembu, Margaret

    2015-01-01

    In 2001, the Meeting of the COMESA Ministers of Agriculture raised concerns that proliferation of genetically modified organisms (GMOs) could impact significantly on trade and food security in the region. This triggered studies on a regional approach to biotechnology and biosafety policy in Eastern and Southern Africa. The studies and stakeholder consultations revealed that farm incomes would increase if they switched from conventional varieties of cotton and maize to genetically modified (GM) counterparts. Commercial risks associated with exports to GM sensitive destinations, e.g., EU were negligible. Intra-regional trade would be affected since exports of GM sensitive commodities, such as maize, cotton, and soya bean, mainly go to other African countries. These findings justified the need to consider a regional approach to biosafety and led to the drafting of a regional policy in 2009. The draft policies were discussed in regional and national workshops between 2010 and 2012 for wider ownership. The workshops involved key stakeholders including ministries of agriculture, trade, environment, national biosafety focal points, biosafety competent authorities, academia, seed traders, millers, the media, food relief agencies, the industry, civil society, competent authorities, and political opinion leaders. The COMESA Council of Ministers in February 2014 adopted the COMESA policy on biotechnology and biosafety that takes into account the sovereign right of each member state. Key provisions of the policy include recognition of the benefits and risks associated with GMOs; establishment of a regional-level biosafety risk-assessment system; national-level final decision, and capacity building assistance to member states. The policies are the first regional effort in Africa to develop a coordinated mechanism for handling biosafety issues related to GMO use. A regional approach to biotechnology and biosafety is expected to foster inter-country cooperation through the

  19. Laboratory-associated infections and biosafety.

    OpenAIRE

    Sewell, D L

    1995-01-01

    An estimated 500,000 laboratory workers in the United States are at risk of exposure to infectious agents that cause disease ranging from inapparent to life-threatening infections, but the precise risk to a given worker unknown. The emergence of human immunodeficiency virus and hantavirus, the continuing problem of hepatitis B virus, and the reemergence of Mycobacterium tuberculosis have renewed interest in biosafety for the employees of laboratories and health care facilities. This review ex...

  20. Biosafety and biosecurity in veterinary laboratories

    Energy Technology Data Exchange (ETDEWEB)

    Finley, Melissa R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Astuto-Gribble, Lisa M. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Brass, Van Hildren [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2016-08-01

    Here, with recent outbreaks of MERS-Cov, Anthrax, Nipah, and Highly Pathogenic Avian Influenza, much emphasis has been placed on rapid identification of infectious agents globally. As a result, laboratories are building capacity, conducting more advanced and sophisticated research, increasing laboratory staff, and establishing collections of dangerous pathogens in an attempt to reduce the impact of infectious disease outbreaks and characterize disease causing agents. With this expansion, the global laboratory community has started to focus on laboratory biosafety and biosecurity to prevent the accidental and/or intent ional release o f these agents. Laboratory biosafety and biosecurity systems are used around the world to help mit igate the risks posed by dangerous pathogens in the laboratory. Veterinary laboratories carry unique responsibilities to workers and communities to safely and securely handle disease causing microorganisms. Many microorganisms studied in veterinary laboratories not only infect animals, but also have the potential to infect humans. This paper will discuss the fundamentals of laboratory biosafety and biosecurity.

  1. National Biocontainment Training Center

    Science.gov (United States)

    2015-02-01

    BSL3 didactic BSL3 practical 1 1 Central Public Health Laboratory Ministry of Health and Population, Cairo, Egypt World Health Organization...courses taken. As expected the number of trainees follows the normal biosafety level pyramid with a large amount of BSL-2 trainees and then fewer as the...Distribution of trainees based on the courses taken. As expected the number of trainees follows the normal biosafety level pyramid with a large

  2. [Viral biosafety, biosecurity, and bioterrorism].

    Science.gov (United States)

    Garin, D

    2010-02-01

    Intentional release of infectious agents has always been considered as a possible weapon. Today this risk has expanded from use for wartime mass destruction to small-scale terrorist acts. Viruses, some of tropical origin, constitute a special biological hazard for several reasons: great infectious potential, adaptability to the host, difficulty for diagnosis in the hospital, and absence of specific treatment for the main agents involved. Handling of the dangerous biological agents requires special biocontainment laboratories equipped and classified according to increasing risk up to level 4. This article discusses the modalities of classification.

  3. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures.

    Science.gov (United States)

    Byrum, Russell; Keith, Lauren; Bartos, Christopher; St Claire, Marisa; Lackemeyer, Matthew G; Holbrook, Michael R; Janosko, Krisztina; Barr, Jason; Pusl, Daniela; Bollinger, Laura; Wada, Jiro; Coe, Linda; Hensley, Lisa E; Jahrling, Peter B; Kuhn, Jens H; Lentz, Margaret R

    2016-10-03

    Medical imaging using animal models for human diseases has been utilized for decades; however, until recently, medical imaging of diseases induced by high-consequence pathogens has not been possible. In 2014, the National Institutes of Health, National Institute of Allergy and Infectious Diseases, Integrated Research Facility at Fort Detrick opened an Animal Biosafety Level 4 (ABSL-4) facility to assess the clinical course and pathology of infectious diseases in experimentally infected animals. Multiple imaging modalities including computed tomography (CT), magnetic resonance imaging, positron emission tomography, and single photon emission computed tomography are available to researchers for these evaluations. The focus of this article is to describe the workflow for safely obtaining a CT image of a live guinea pig in an ABSL-4 facility. These procedures include animal handling, anesthesia, and preparing and monitoring the animal until recovery from sedation. We will also discuss preparing the imaging equipment, performing quality checks, communication methods from "hot side" (containing pathogens) to "cold side," and moving the animal from the holding room to the imaging suite.

  4. Experiences from the implementation of a biosafety system in Slovenia.

    Science.gov (United States)

    Milavec, Mojca; Racman, Darja Stanic

    2007-09-01

    The development and implementation of an effective national biosafety system is important for several key reasons: to ensure safe access to products of modern biotechnology, to build public confidence, to encourage the growth of domestic modern biotechnology, and to comply with international standards and agreements. There is no single best approach in the development and implementation of a national biosafety system and each country is faced with unique challenges. Slovenia is a small country and a new EU Member State. However, it has developed and implemented an efficient national biosafety system. The key elements of this system are administrative procedure, risk assessment, enforcement, and public participation and information.

  5. Progress and Challenges for the Implementation of the Common Market for Eastern and Southern Africa (COMESA Policy on Biotechnology and Biosafety

    Directory of Open Access Journals (Sweden)

    Michael eWaithaka

    2015-07-01

    Full Text Available In 2001, the Meeting of the COMESA Ministers of Agriculture raised concerns that proliferation of genetically modified organisms (GMOs could impact significantly on trade and food security in the region. This triggered studies on a regional approach to biotechnology and biosafety policy in Eastern and Southern Africa. The studies and stakeholder consultations revealed that farm incomes would increase if they switched from conventional varieties of cotton and maize to GM counterparts. Commercial risks associated with exports to GM sensitive destinations e.g., EU were negligible. Intra-regional trade would be affected since exports of GM sensitive commodities such as maize, cotton and soya bean mainly go to other African countries. These findings justified the need to consider a regional approach to biosafety and led to the drafting of a regional policy in 2009. The draft policies were discussed in regional and national workshops between 2010 and 2012 for wider ownership. The workshops involved key stakeholders including ministries of agriculture, trade, environment, national biosafety focal points, biosafety competent authorities, academia, seed traders, millers, the media, food relief agencies, the industry, civil society, competent authorities and political opinion leaders. The COMESA Council of Ministers in February 2014 adopted the COMESA policy on biotechnology and biosafety that takes into account the sovereign right of each member state. Key provisions of the policy include: recognition both to the benefits and risks associated with GMOs; establishment of regional-level biosafety risk assessment system; national level final decision, and capacity building assistance to member states. The policies are the first regional effort in Africa to develop a coordinated mechanism for handling biosafety issues related to GMO use. Regional approach to biosafety is expected to foster inter-country cooperation through the sharing of knowledge, expertise

  6. Laboratory biosafety for handling emerging viruses

    Directory of Open Access Journals (Sweden)

    I. Made Artika

    2017-05-01

    Full Text Available Emerging viruses are viruses whose occurrence has risen within the past twenty years, or whose presence is likely to increase in the near future. Diseases caused by emerging viruses are a major threat to global public health. In spite of greater awareness of safety and containment procedures, the handling of pathogenic viruses remains a likely source of infection, and mortality, among laboratory workers. There is a steady increase in both the number of laboratories and scientist handling emerging viruses for diagnostics and research. The potential for harm associated to work with these infectious agents can be minimized through the application of sound biosafety concepts and practices. The main factors to the prevention of laboratory-acquired infection are well-trained personnel who are knowledgable and biohazard aware, who are perceptive of the various ways of transmission, and who are professional in safe laboratory practice management. In addition, we should emphasize that appropriate facilities, practices and procedures are to be used by the laboratory workers for the handling of emerging viruses in a safe and secure manner. This review is aimed at providing researchers and laboratory personnel with basic biosafety principles to protect themselves from exposure to emerging viruses while working in the laboratory. This paper focuses on what emerging viruses are, why emerging viruses can cause laboratory-acquired infection, how to assess the risk of working with emerging viruses, and how laboratory-acquired infection can be prevented. Control measures used in the laboratory designed as such that they protect workers from emerging viruses and safeguard the public through the safe disposal of infectious wastes are also addressed.

  7. The need for biosafety regulation in developing countries: Benefits ...

    African Journals Online (AJOL)

    Jane

    2011-09-30

    Sep 30, 2011 ... Key words: Biotechnology, biosafety, developing countries, benefits, risks and controversies. ... biotechnology concerns relate more to human health and ..... animals in laboratory, when they are tested outdoors, and when they ...

  8. Biosafety regulatory regimes in East and Central Africa

    African Journals Online (AJOL)

    IDDA

    countries covered in this study are Tanzania, Kenya, Uganda, Rwanda, ... A critical look is given to the existing biosafety frameworks, pinpointing their weaknesses and giving suggestions on what could be done to address the shortfalls.

  9. Biosafety Management of Genetically Modified Crops (China) | IDRC ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Biosafety Management of Genetically Modified Crops (China). Since 1990, China's ... Country(s). China, Far East Asia, Central Asia, South Asia ... Call for new OWSD Fellowships for Early Career Women Scientists now open. In partnership ...

  10. Biosafety- A Regulatory Primer for DOE/NNSA Sites

    Energy Technology Data Exchange (ETDEWEB)

    Siegel, Dina Mary [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-05-04

    The objectives of a biological safety program, and this are to ensure that work with biological materials are conducted in compliance with applicable biosafety standards and ensure that protection of workers, facilities, the public, and the environment.

  11. Assessment of biosafety precautions in Khartoum state diagnostic laboratories, Sudan

    OpenAIRE

    Elduma, Adel Hussein

    2012-01-01

    Background This study was conducted to evaluate the biosafety precautions that applied by diagnostic laboratories in Khartoum state, 2009. Methods A total number of 190 laboratories were surveyed about their compliance with standard biosafety precautions. These laboratories included 51 (27%) laboratories from government, 75 (39%) from private sectors and 64 (34%) laboratories belong to organization providing health care services. Results The study found that 32 (16.8%) of laboratories appoint...

  12. A survey of Asian life scientists :the state of biosciences, laboratory biosecurity, and biosafety in Asia.

    Energy Technology Data Exchange (ETDEWEB)

    Gaudioso, Jennifer Marie

    2006-02-01

    Over 300 Asian life scientists were surveyed to provide insight into work with infectious agents. This report provides the reader with a more complete understanding of the current practices employed to study infectious agents by laboratories located in Asian countries--segmented by level of biotechnology sophistication. The respondents have a variety of research objectives and study over 60 different pathogens and toxins. Many of the respondents indicated that their work was hampered by lack of adequate resources and the difficulty of accessing critical resources. The survey results also demonstrate that there appears to be better awareness of laboratory biosafety issues compared to laboratory biosecurity. Perhaps not surprisingly, many of these researchers work with pathogens and toxins under less stringent laboratory biosafety and biosecurity conditions than would be typical for laboratories in the West.

  13. Biosafety initiatives in BMENA region: identification of gaps and advances

    Directory of Open Access Journals (Sweden)

    Erum eKhan

    2016-03-01

    Full Text Available AbstractIntroduction: The objectives of this study were to identify and assess the impact of capacity-building biosafety initiatives and programs that have taken place in the broader Middle East and north Africa (BMENA region between 2001-13, to highlight gaps that require further development, and to suggest sustainable ways to build cooperative regional biosafety opportunities. Methods: A cross sectional study was conducted with two aspects 1 thorough desktop review of literature for all biosafety / biosecurity related activities in the study countries such as seminars, conferences, workshops, policy documents, technology transfer, sustained scientific endeavours between countries etc.; and 2 an online survey of scientists in countries in the region to get first-hand information about biosafety and biosecurity initiatives and gaps in their country. Results: A total of 1832 initiatives of biosafety / biosecurity were recorded from 97 web-links,70.68% (n=1295 initiatives were focused on raising general awareness among the scientific community about biosafety/biosecurity/biocontainment. The most frequent areas of interest were biorisk management in biomedical and biotechnology laboratories 13% (n=239, followed by Living Modified Organisms (LMO’s 9.17% (n=168. Hands-on training accounted for 2.67% (n=49 of initiatives. On line survey results confirmed desktop review findings, however the response rate was 11%.

  14. Biosafety Initiatives in BMENA Region: Identification of Gaps and Advances.

    Science.gov (United States)

    Khan, Erum; Ahmed, Nayla; Temsamani, Khalid R; El-Gendy, Atef; Cohen, Murray; Hasan, Ariba; Gastfriend, Hilliard; Cole, Jennifer

    2016-01-01

    The objectives of this study were to identify and assess the impact of capacity-building biosafety initiatives and programs that have taken place in the broader Middle East and North Africa (BMENA) region between 2001 and 2013, to highlight gaps that require further development, and to suggest sustainable ways to build cooperative regional biosafety opportunities. A cross-sectional study was conducted with two aspects (1) thorough desktop review of literature for all biosafety/biosecurity-related activities in the study countries, such as seminars, conferences, workshops, policy documents, technology transfer, sustained scientific endeavors between countries, etc. and (2) an online survey of scientists in countries in the region to get first-hand information about biosafety and biosecurity initiatives and gaps in their country. A total of 1832 initiatives of biosafety/biosecurity were recorded from 97 web links; 70.68% (n = 1295) initiatives were focused on raising general awareness among the scientific community about biosafety/biosecurity/biocontainment. The most frequent areas of interest were biorisk management in biomedical and biotechnology laboratories 13% (n = 239), followed by living modified organisms (LMOs) 9.17% (n = 168). Hands-on training accounted for 2.67% (n = 49) of initiatives. Online survey results confirmed desktop review findings; however, the response rate was 11%.

  15. Assessment of biosafety precautions in Khartoum state diagnostic laboratories, Sudan.

    Science.gov (United States)

    Elduma, Adel Hussein

    2012-01-01

    This study was conducted to evaluate the biosafety precautions that applied by diagnostic laboratories in Khartoum state, 2009. A total number of 190 laboratories were surveyed about their compliance with standard biosafety precautions. These laboratories included 51 (27%) laboratories from government, 75 (39%) from private sectors and 64 (34%) laboratories belong to organization providing health care services. The study found that 32 (16.8%) of laboratories appointed biosafety officers. Only, ten (5.2%) participated in training about response to fire emergency, and 28 (14.7%) reported the laboratory accident occurred during work. 45 (23.7%) laboratories had a written standard operation procedures (SOPs), and 35 (18.4%) had written procedures for the lean-up of spills. Moreover, biosafety cabinet was found in 11 (5.8%) laboratories, autoclave in 28 (14.7%) and incinerator in only two (1.1%) laboratories. Sharp disposable containers were found in 84 (44.2%). Fire alarm system was found in 2 (1.1%) laboratories, fire extinguisher in 39 (20.5%) laboratories, and fire emergency exit found in 14 (7.4%) laboratories. Furthermore, 19 (10%) laboratories had a hepatitis B virus vaccination programme, 5 (6.2%) applied BCG vaccine, and 2 (1.1%0) vaccinated the staff against influenza. The study concluded that the standards biosafety precautions adopted by the diagnostic laboratories in Khartoum state was very low. Further, the laboratory personnel awareness towards biosafety principles implementation was very low too.

  16. [Education on biosafety and bioethics: necessary articulation in biotechnology].

    Science.gov (United States)

    Bonis, Marcos De; Costa, Marco Antonio Ferreira da

    2009-01-01

    Science education has been discussed in some segments of the society and, international organizations have encouraged nations to invest in this strategic area. In this context, education in bioethics and biosafety explores a rich content on prevention, standards and ethical principles which serve to guide the paths track by biotechnology. The recovery of bioethics and biosafety, as part of an educational policy scientific, effective and consistent, can stimulate the formation of individuals with a scientific and citizen awareness, in a position to participate on ethical and technological issues produced by biotechnology.

  17. Assessment of biosafety precautions in Khartoum state diagnostic ...

    African Journals Online (AJOL)

    Background: This study was conducted to evaluate the biosafety precautions that applied by diagnostic laboratories in Khartoum state, 2009. Methods: A total number of 190 laboratories were surveyed about ... Sharp disposable containers were found in 84 (44.2%). Fire alarm system was found in 2 (1.1%) laboratories, fire ...

  18. [Biosafety and biosecurity in the medical laboratory. Update and trends].

    Science.gov (United States)

    Ionescu, G; Neguţ, M; Combiescu, A A

    2007-01-01

    Biosafety includes the protective measures against the risks of contamination with pathogen germs in the laboratories that handle pathogens, or stock or manipulate potentially contaminated products, or perform microbiological tests for medical or scientific research purposes, as well as the means of protecting the environment and the human collectivities against hazard contaminations that have as starting point these laboratories. Besides, lately, a new notion emerged, that of biosecurity, which refers to the sum of measures designed to protect workers, environment and population against the loss, theft, use and release in the environment of pathogenic biological agents. The work overviews the present concerns for the regulation of these two notions and the way in which a system for the management of the biological risks in a laboratory that handles pathogens should be documented and implemented. The need for the continuous professional training of the staff and for the establishment of individual and collective responsibilities for preventing biosafety incidents and trespassing biosecurity rules are as well emphasized. The main biosafety measures are pointed out and a series of considerations regarding biosafety and bioterrorism in correlation with the medical laboratory are as well mentioned.

  19. Biosafety regulations in Brazil | Sampaio | African Crop Science ...

    African Journals Online (AJOL)

    Journal Home > Vol 3, No 3 (1995) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. Biosafety regulations in Brazil. MJA Sampaio. Abstract. No Abstract. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL ...

  20. 78 FR 9828 - Establishment of User Fees for Filovirus Testing of Nonhuman Primate Liver Samples

    Science.gov (United States)

    2013-02-12

    ... specifically for a ``filovirus testing facility'' because laboratories, especially the Biosafety Level 4 (BSL-4... a price that may be below standard commercial market fees, or free. The fee HHS/CDC intends to...

  1. International Society for Analytical Cytology biosafety standard for sorting of unfixed cells.

    Science.gov (United States)

    Schmid, Ingrid; Lambert, Claude; Ambrozak, David; Marti, Gerald E; Moss, Delynn M; Perfetto, Stephen P

    2007-06-01

    Cell sorting of viable biological specimens has become very prevalent in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the use of sorters are paramount. To this end the International Society of Analytical Cytology (ISAC) took a lead in establishing biosafety guidelines for sorting of unfixed cells (Schmid et al., Cytometry 1997;28:99-117). During the time period these recommendations have been available, they have become recognized worldwide as the standard practices and safety precautions for laboratories performing viable cell sorting experiments. However, the field of cytometry has progressed since 1997, and the document requires an update. Initially, suggestions about the document format and content were discussed among members of the ISAC Biosafety Committee and were incorporated into a draft version that was sent to all committee members for review. Comments were collected, carefully considered, and incorporated as appropriate into a draft document that was posted on the ISAC web site to invite comments from the flow cytometry community at large. The revised document was then submitted to ISAC Council for review. Simultaneously, further comments were sought from newly-appointed ISAC Biosafety committee members. This safety standard for performing viable cell sorting experiments was recently generated. The document contains background information on the biohazard potential of sorting and the hazard classification of infectious agents as well as recommendations on (1) sample handling, (2) operator training and personal protection, (3) laboratory design, (4) cell sorter set-up, maintenance, and decontamination, and (5) testing the instrument for the efficiency of aerosol containment. This standard constitutes an updated and expanded revision of the 1997 biosafety guideline document. It is intended to provide

  2. [Dimensional analysis of the concept of biosafety due to bioterrorism].

    Science.gov (United States)

    Bernard, Laurence; Shaha, Maya

    2014-03-01

    In recent years with the strengthening of the discourse surrounding the biological risk of bioterrorist nature, the concept of biosafety emerged gradually. A dimensional analysis was used to contextualize the concept. Initially, biosafety was essentially a technical term related to the risks of contamination in laboratories or food industry and then be used to protect biodiversity against the spread of genetically modified organisms (GMOs) into the environment. Now, it is increasingly used in reference to the prevention and infections control, even though its use remains marginal. However, biosecurity may be defined as the security of life and therefore affect the safety devices participating in the government of bodies and power over life. A more critical approach including social and political dimensions within a Foucauldian perspective is needed to expand the scope of the biosecurity concept up to biological hazards constructs.

  3. 77 FR 7109 - Establishment of User Fees for Filovirus Testing of Nonhuman Primate Liver Samples

    Science.gov (United States)

    2012-02-10

    ... quarantine standards under 42 CFR part 71 for continued registration as an importer of NHPs (2). On March 23... reagents requires a biosafety level 4 laboratory (BSL-4). A BSL- 4 laboratory is also required during part..., establishment of standards, and regulation. Full costs are determined based on the best available records of the...

  4. 77 FR 6971 - Establishment of User Fees for Filovirus Testing of Nonhuman Primate Liver Samples

    Science.gov (United States)

    2012-02-10

    ... Director on March 15, 1990, that they must comply with specific isolation and quarantine standards under 42... biosafety level 4 laboratory (BSL-4). A BSL-4 laboratory is also required during part of the testing... and supervisory costs; and the costs of enforcement, collection, research, establishment of standards...

  5. The Role of Trust Building in the Development of Biosafety Regulations in Kenya - Comment

    Directory of Open Access Journals (Sweden)

    Justin Mabeya, Peter A. Singer and Obidimma C. Ezezika

    2010-09-01

    Full Text Available The potential of biotechnology to contribute to the reduction of hunger, malnutrition and poverty in Africa can only be realised with the presence of biosafety legislation. Recently, Kenya enacted the Biosafety Act 2008 after more than six years of stakeholder engagement with farmers, academicians, researchers, members of the community, funders, regulators, and private sector players. In this article, we highlight the challenges and importance of trust among stakeholders in the development and implementation of biosafety legislation in Kenya . We show how open stewardship by government, time investment, consensus building and sustained stakeholder engagement could be key aspects in building trust among stakeholders in the development of national biosafety regulations. Through our analyses of the process of development of Kenya biosafety regulations, we provide a set of guidelines that could help other African countries develop and improve stakeholder trust in developing biosafety regulations.

  6. First draft genome sequencing of indole acetic acid producing and plant growth promoting fungus Preussia sp. BSL10.

    Science.gov (United States)

    Khan, Abdul Latif; Asaf, Sajjad; Khan, Abdur Rahim; Al-Harrasi, Ahmed; Al-Rawahi, Ahmed; Lee, In-Jung

    2016-05-10

    Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the first time. The Illumina based sequence analysis reveals an approximate genome size of 31.4Mbp for Preussia sp. BSL10. Based on ab initio gene prediction, total 32,312 coding sequences were annotated consisting of 11,967 coding genes, pseudogenes, and 221 tRNA genes. Furthermore, 321 carbohydrate-active enzymes were predicted and classified into many functional families. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. DIFFERENCES BETWEEN AMERICAN SIGN LANGUAGE (ASL AND BRITISH SIGN LANGUAGE (BSL

    Directory of Open Access Journals (Sweden)

    Zora JACHOVA

    2008-06-01

    Full Text Available In the communication of deaf people between them­selves and hearing people there are three ba­sic as­pects of interaction: gesture, finger signs and writing. The gesture is a conditionally agreed manner of communication with the help of the hands followed by face and body mimic. The ges­ture and the move­ments pre-exist the speech and they had the purpose to mark something, and later to emphasize the speech expression.Stokoe was the first linguist that realised that the signs are not a whole that can not be analysed. He analysed signs in insignificant parts that he called “chemeres”, and many linguists today call them pho­nemes. He created three main phoneme catego­ries: hand position, location and movement.Sign languages as spoken languages have back­ground from the distant past. They developed par­allel with the development of spoken language and undertook many historical changes. Therefore, to­day they do not represent a replacement of the spoken language, but are languages themselves in the real sense of the word.Although the structures of the English language used in USA and in Great Britain is the same, still their sign languages-ASL and BSL are different.

  8. [Knowledge and adherence to bio-safety measures and biological accidents by nursing students during their clinical practice].

    Science.gov (United States)

    Merino-de la Hoz, Felicitas; Durá-Ros, María Jesús; Rodríguez-Martín, Elías; González-Gómez, Silvia; Mariano López-López, Luis; Abajas-Bustillo, Rebeca; de la Horra-Gutiérrez, Inmaculada

    2010-01-01

    To identify the degree of knowledge and performance of bio-safety measures by nursing students and knowing the type of biological accidents suffered during their clinical practice. A cross-sectional study was conducted on the students of three Nursing courses held in May of 2008. Data was collected by an anonymous self-administered questionnaire, with a return of 54%. A total of 97% of students seemed to know the standard biosafety measures, and all of them (100%) stated that those measures must be applied to every patient. However, the reality of clinical practice shows that biosafety measures are only partially applied. An average of 60.2% implement the personal hygiene measures, 66.1% use physical barriers, and 44% use sharp materials safely. Around 32.25% of the students have suffered some biological accident, with a greater incidence in the second year: administering injections (24%), drawing blood samples with Venojet needles (18%) and recapping used needles (17%). The high level of knowledge shown by the students on standard precautions is not always shown in clinical practice. There are significant deficiencies in student safety practices: recapping of used needles continues to be one of the most common risk practices carried out. Copyright (c) 2009 Elsevier España, S.L. All rights reserved.

  9. A Biosafety Level 2 Virology Lab for Biotechnology Undergraduates

    Science.gov (United States)

    Matza-Porges, Sigal; Nathan, Dafna

    2017-01-01

    Medical, industrial, and basic research relies heavily on the use of viruses and vectors. Therefore, it is important that bioscience undergraduates learn the practicalities of handling viruses. Teaching practical virology in a student laboratory setup presents safety challenges, however. The aim of this article is to describe the design and…

  10. 78 FR 110 - Supplemental Record of Decision; Final Supplementary Risk Assessment for the Boston University...

    Science.gov (United States)

    2013-01-02

    ..., questions were raised specifically about the potential risks of the biosafety level 4 (BSL-4) laboratory. To... epidemiology, risk assessment, environmental justice, risk communications, biosafety, and infectious disease... functional personal protective equipment, and standard operating procedures were not taken into account in...

  11. The Programs for Strengthening Biosafety and Biosecurity in Georgia

    International Nuclear Information System (INIS)

    Kutateladze, M.

    2007-01-01

    The difficulties connected with the political changes due to the breakdown of Soviet system caused serious problems in biosafety and security in Georgia. During last 10-12 years, lack of state financing caused destruction of the systems of biosafety in the relevant Institutions - the system became formal and completely damaged. The program for prevention of biological weapons (BW) proliferation operates in Georgia since 2002. The agreement between United States and Georgia covers several issues, including prevention the proliferation of biological weapons technology, pathogenic strains and their expertise at the source. Department of Defense of the USA supports the country to consolidate especially dangerous pathogens (EDPs) into safe and secure central reference laboratories, improve our capabilities to detect and respond to disease outbreaks caused by the EDP, integrate Georgian scientists into the international scientific community and eliminate BW infrastructure and technology. Elimination of BW infrastructure includes dismantle and elimination of biological threat agent materials, dual-use equipment and associated infrastructure. Biosecurity and Biosafety involve implementation of technical enhancements to meet and maintain US standards, create a personal reliability program to reduce the release of pathogens and secure a safer working environment for personnel. Currently, two projects are funded through the Cooperative Biological Research (CBR) in Georgia - to study the ecology, biodiversity, genetic clustering and virulence of Yersinia pestis and Vibrio spp. These projects are performed at the National Center for Disease Control and Medical statistics (NCDC) and G. Eliava Institute of Bacteriophages, Microbiology and Virology. These projects are carried out due to the fruitful collaboration between Georgian and American scientists. Threat Agent Detection and Response (TADR) system provides enhanced reporting, detection and response for human and animal EDPs

  12. The Bioethics and Biosafety technosciences and transcendence of limits

    International Nuclear Information System (INIS)

    Newton, Aquiles von Zuben

    2008-01-01

    Bioethics as a cultural phenomenon is nowadays presented as a paradigmatic locus of reflection, critical analysis, inquiries and debates about ethical problems and moral dilemmas provoked by scientific researches in the field of Biotechnology, with its innovations and applications. Humanity, since the middle of X X Th Century, lives under uncertainty and fear. Bioethics responds to the need of a ethical reflection which follows such inquiries and technological applications. One of the subjects of Bioethics is the biosafety, which deals with biohazards. In this process, there is a privileged place many questions such as technological evaluation, risk management and, in a special way, the precautionary principle. This study focus on these questions

  13. Air flow optimization for energy efficient blower of biosafety cabinet class II A2

    Science.gov (United States)

    Ibrahim, M. D.; Mohtar, M. Z.; Alias, A. A.; Wong, L. K.; Yunos, Y. S.; Rahman, M. R. A.; Zulkharnain, A.; Tan, C. S.; Thayan, R.

    2017-04-01

    An energy efficient Biosafety Cabinet (BSC) has become a big challenge for manufacturers to develop BSC with the highest level of protection. The objective of research is to increase air flow velocity discharge from centrifugal blower. An aerodynamic duct shape inspired by the shape of Peregrine Falcon’s wing during diving flight is added to the end of the centrifugal blower. Investigation of air movement is determined by computational fluid dynamics (CFD) simulation. The results showed that air velocity can be increased by double compared to typical manufactured BSC and no air recirculation. As conclusion, a novel design of aerodynamic duct shape successfully developed and proved that air velocity can be increase naturally with same impeller speed. It can contribute in increasing energy efficiency of the centrifugal blower. It is vital to BSC manufacturer and can be apply to Heating, Air Ventilation and Air Conditioning (HVAC) industries.

  14. RNA-dependent RNA polymerase of hepatitis C virus binds to its coding region RNA stem-loop structure, 5BSL3.2, and its negative strand.

    Science.gov (United States)

    Kanamori, Hiroshi; Yuhashi, Kazuhito; Ohnishi, Shin; Koike, Kazuhiko; Kodama, Tatsuhiko

    2010-05-01

    The hepatitis C virus NS5B RNA-dependent RNA polymerase (RdRp) is a key enzyme involved in viral replication. Interaction between NS5B RdRp and the viral RNA sequence is likely to be an important step in viral RNA replication. The C-terminal half of the NS5B-coding sequence, which contains the important cis-acting replication element, has been identified as an NS5B-binding sequence. In the present study, we confirm the specific binding of NS5B to one of the RNA stem-loop structures in the region, 5BSL3.2. In addition, we show that NS5B binds to the complementary strand of 5BSL3.2 (5BSL3.2N). The bulge structure of 5BSL3.2N was shown to be indispensable for tight binding to NS5B. In vitro RdRp activity was inhibited by 5BSL3.2N, indicating the importance of the RNA element in the polymerization by RdRp. These results suggest the involvement of the RNA stem-loop structure of the negative strand in the replication process.

  15. Databases on biotechnology and biosafety of GMOs.

    Science.gov (United States)

    Degrassi, Giuliano; Alexandrova, Nevena; Ripandelli, Decio

    2003-01-01

    Due to the involvement of scientific, industrial, commercial and public sectors of society, the complexity of the issues concerning the safety of genetically modified organisms (GMOs) for the environment, agriculture, and human and animal health calls for a wide coverage of information. Accordingly, development of the field of biotechnology, along with concerns related to the fate of released GMOs, has led to a rapid development of tools for disseminating such information. As a result, there is a growing number of databases aimed at collecting and storing information related to GMOs. Most of the sites deal with information on environmental releases, field trials, transgenes and related sequences, regulations and legislation, risk assessment documents, and literature. Databases are mainly established and managed by scientific, national or international authorities, and are addressed towards scientists, government officials, policy makers, consumers, farmers, environmental groups and civil society representatives. This complexity can lead to an overlapping of information. The purpose of the present review is to analyse the relevant databases currently available on the web, providing comments on their vastly different information and on the structure of the sites pertaining to different users. A preliminary overview on the development of these sites during the last decade, at both the national and international level, is also provided.

  16. Marburg biosafety and biosecurity scale (MBBS): a framework for risk assessment and risk communication.

    Science.gov (United States)

    Dickmann, Petra; Apfel, Franklin; Biedenkopf, Nadine; Eickmann, Markus; Becker, Stephan

    2015-01-01

    Current risk assessment and risk communication of biosafety and biosecurity concerns lack a convenient metric and conceptual framework. The absence of such a systematic tool makes communication more difficult and can lead to ambiguous public perception of and response to laboratory biosafety incidents and biosecurity threats. A new 7-category scoring scale is proposed for incidents and situations in laboratories related to the handling of human and animal pathogens. The scale aims to help clarify risk categories, facilitate coordination and communication, and improve public understanding of risk related to biosafety and biosecurity.

  17. Re-Engineering Biosafety Regulations In India: Towards a Critique of Policy, Law and Prescriptions

    Directory of Open Access Journals (Sweden)

    A. Damodaran

    2005-06-01

    Full Text Available This article surveys the structure and essence of India’s biosafety regulations from an evolutionary perspective. After detailing the processes associated with the biosafety law and guidelines in the country, this article looks critically at recent efforts to re-engineer the regulations. It is argued that India’s biosafety regulations should move towards a more inclusive approach, which will facilitate transparent and informed decision-making, based on stakeholder-convergence. It is also suggested that the entire spectrum of laws and regulations that have a direct or indirect bearing on biosafety in India, need to be explored so that greater coherence could be secured in the management of biotechnology products that are sensitive to the environment. Drawing from the experience of the Bt cotton case, the article advocates a greater role for civil society and grassroots organizations.

  18. Mesoporous Silica and Organosilica Nanoparticles: Physical Chemistry, Biosafety, Delivery Strategies, and Biomedical Applications

    KAUST Repository

    Croissant, Jonas G.; Fatieiev, Yevhen; Almalik, Abdulaziz; Khashab, Niveen M.

    2017-01-01

    organosilica, and fully hybridized organosilica (periodic mesoporous organosilicas) governs not only the physico-chemical properties but also the biosafety of the nanoparticles. The impact of the hybridization on the biocompatibility, protein corona

  19. State-of-the-Art in Biosafety and Biosecurity in European Countries

    OpenAIRE

    Bielecka, Anna; Mohammadi, Ali Akbar

    2014-01-01

    The terms biosafety and biosecurity are widely used in different concepts and refer not only to protection of human beings and their surrounding environment against hazardous biological agent, but also to global disarmament of weapons of mass destruction. As a result, the biosafety and biosecurity issues should be considered interdisciplinary based on multilateral agreements against proliferation of biological weapons, public health and environmental protection. This publication presents info...

  20. A Security plan for LMOs - concentrated on environmental policy of Biosafety Protocol

    Energy Technology Data Exchange (ETDEWEB)

    Park, Yong Ha [Korea Environment Institute, Seoul (Korea)

    1998-12-01

    Biotechnology industry in Korea is raised by the national support. Also, Korea imports 70% of entire agricultural products. Considering the present situation in Korea, signing a Biosafety Protocol is necessary to prevent harm by LMOs and to protect associated biotechnological industry. Therefore, the problems on signing Biosafety Protocol were analyzed and the environmental policy to be pursued was proposed. This study result will be a cornerstone to prepare a definite environmental policy by government. 54 refs., 7 figs., 27 tabs.

  1. Toward a workable biosafety system for regulating genetically modified organisms in Ethiopia: balancing conservation and competitiveness.

    Science.gov (United States)

    Abraham, Adane

    2013-01-01

    On September 9, 2009, Ethiopia enacted a highly restrictive biosafety law firmly based on precautionary principles as a foundation for its GMO regulation system. Its drafting process, led by the country's Environmental Protection Authority, was judged as biased, focusing only on protecting the environment from perceived risks, giving little attention to potential benefits of GMOs. Many of its provisions are very stringent, exceeding those of Cartagena Protocol on Biosafety, while others cannot be fulfilled by applicants, collectively rendering the emerged biosafety system unworkable. These provisions include requirements for advance informed agreement and rigorous socioeconomic assessment in risk evaluation for all GMO transactions, including contained research use-which requires the head of the competent national authority of the exporting country to take full responsibility for GMO-related information provided-and stringent labeling, insurance and monitoring requirements for all GMO activities. Furthermore, there is no provision to establish an independent national biosafety decision-making body(ies). As a result, foreign technology owners that provide highly demanded technologies like Bt cotton declined to work with Ethiopia. There is a fear that the emerged biosafety system might also continue to suppress domestic genetic engineering research and development. Thus, to benefit from GMOs, Ethiopia has to revise its biosafety system, primarily by making changes to some provisions of the law in a way that balances its diverse interests of conserving biodiversity, protecting the environment and enhancing competition in agricultural and other economic sectors.

  2. The biosafety of X-ray in bed

    International Nuclear Information System (INIS)

    Costa, A.S.; Vinco, Y.C.; Machado, C.P.

    2015-01-01

    This work aims to raise awareness on biosafety that the professional radiology needs to develop, for their own protection as well as the patient in bed during the examination of the X-ray. Assess why the use of many artifacts and discuss their safe use, the conditions necessary for operating activities that employ radioactive and radiological techniques are adopted for the benefit of society. Taking also into account the protection of workers, the public, and the patient environment. This study aims to evaluate the knowledge of technical professionals working in the field-performing x -rays in bed, currently academic course in radiology technologist. The results obtained show that 67 % of technicians, technologists’ future, use the personal protective equipment, and 25 % sometimes and never use 8 %, 92 %. Answered that in the period that is being performed on X -ray examination bed, but there are others bedridden in the same environment, with 88 % of patients in bed in bed not receive personal protective equipment nor collective protection equipment. Thus, we conclude that most technicians have cognition existing risks, so the individual protection measures are being carried out, but not by all. What still leaves to be desired is the protection of the patient in bed. These professionals need to be encouraged to study, so that we have trained professionals and holders of knowledge, enabling the improvement in labor and protection of professional and patient. (author)

  3. Biossegurança em fonoaudiologia Biosafety in Speech, Language and Hearing Sciences

    Directory of Open Access Journals (Sweden)

    Marcela do Amaral de Albuquerque

    2013-01-01

    indicating its performance. The percentage value of each questionnaire could vary from 0 to 100%, and the greater the percentage obtained, the more knowledge and applicability of standards of biosafety professionals in the clinical routine. Tracks were taken (0-25%, (26-50%, (51-75% and (76-100% to distinguish the level of knowledge and application of precautionary measures by the participants. RESULTS: of one hundred speech therapists assessed by the questionnaires (100%, 1% obtained the percentage in the range (0 to 25%, 45% (26 to 50%, between 50% (51 to 75% and 4% (76 to 100%. CONCLUSION: the most of the professionals who participated knows and applies biosafety measures.

  4. Biosafety capacity building: experiences and challenges from a distance learning approach.

    Science.gov (United States)

    Pertry, Ine; Sabbadini, Silvia; Goormachtig, Sofie; Lokko, Yvonne; Gheysen, Godelieve; Burssens, Sylvia; Mezzetti, Bruno

    2014-01-25

    Biotechnology is revolutionizing industrial and agricultural practice as the number of commercial biotechnology products is increasing each year. Simultaneously, several regulatory approaches are put into place to allow technological advancement while preserving public health and the environment. Developing and/or emerging countries often face major barriers to access biotechnologies and biotechnology derived products as they frequently lack the institutional capacities and professional competence in exercising regulatory oversight. To address this need, intensive biosafety capacity building is required. Different training approaches can be used to train individuals in biosafety ranging from long-term leading to a postgraduate certificate or a Masters degree, to short term courses. In this paper, we discuss the applicability of a different approach to biosafety capacity building based on a distance e-learning system, the UNIDO e-Biosafety program that has been annually organized at the Marche Polytechnic University (MPU) in Italy and Ghent University (UGent) in Belgium since 2006. Even though there are some challenges, we can conclude based on our experience that distance learning in combination with on-campus tuition is amendable for biosafety capacity building. Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.

  5. Agrobacterium-mediated gene transfer in plants and biosafety considerations.

    Science.gov (United States)

    Mehrotra, Shweta; Goyal, Vinod

    2012-12-01

    Agrobacterium, the natures' genetic engineer, has been used as a vector to create transgenic plants. Agrobacterium-mediated gene transfer in plants is a highly efficient transformation process which is governed by various factors including genotype of the host plant, explant, vector, plasmid, bacterial strain, composition of culture medium, tissue damage, and temperature of co-cultivation. Agrobacterium has been successfully used to transform various economically and horticulturally important monocot and dicot species by standard tissue culture and in planta transformation techniques like floral or seedling infilteration, apical meristem transformation, and the pistil drip methods. Monocots have been comparatively difficult to transform by Agrobacterium. However, successful transformations have been reported in the last few years based on the adjustment of the parameters that govern the responses of monocots to Agrobacterium. A novel Agrobacterium transferred DNA-derived nanocomplex method has been developed which will be highly valuable for plant biology and biotechnology. Agrobacterium-mediated genetic transformation is known to be the preferred method of creating transgenic plants from a commercial and biosafety perspective. Agrobacterium-mediated gene transfer predominantly results in the integration of foreign genes at a single locus in the host plant, without associated vector backbone and is also known to produce marker free plants, which are the prerequisites for commercialization of transgenic crops. Research in Agrobacterium-mediated transformation can provide new and novel insights into the understanding of the regulatory process controlling molecular, cellular, biochemical, physiological, and developmental processes occurring during Agrobacterium-mediated transformation and also into a wide range of aspects on biological safety of transgenic crops to improve crop production to meet the demands of ever-growing world's population.

  6. Genome sequence of the photoarsenotrophic bacterium Ectothiorhodospira sp. strain BSL-9, isolated from a hypersaline alkaline arsenic-rich extreme environment

    Science.gov (United States)

    Hernandez-Maldonado, Jaime; Stoneburner, Brendon; Boren, Alison; Miller, Laurence; Rosen, Michael R.; Oremland, Ronald S.; Saltikov, Chad W

    2016-01-01

    The full genome sequence of Ectothiorhodospira sp. strain BSL-9 is reported here. This purple sulfur bacterium encodes an arxA-type arsenite oxidase within the arxB2AB1CD gene island and is capable of carrying out “photoarsenotrophy” anoxygenic photosynthetic arsenite oxidation. Its genome is composed of 3.5 Mb and has approximately 63% G+C content.

  7. [Biosafety provision on handling pathogenic biological agents on the concept of biorisk assessment and management].

    Science.gov (United States)

    Dobrokhotskiĭ, O N; Kolombet, L V

    2010-01-01

    The paper shows it urgent to realize the concept of biological risk assessment and management on handling pathogenic biological agents (PBA). It gives a number of objective reasons that impede development of a methodology to assess laboratory biological risks. A concept of continuous improvement (a process approach) is proposed for use as a biorisk management tool for biosafety assurance when handling PBA. It is demonstrated that development of international cooperation urgently requires that national concepts and standards be harmonized with international regulatory documents on biosafety assurance on handling PBA.

  8. Strengthening the diagnostic capacity to detect Bio Safety Level 3 organisms in unusual respiratory viral outbreaks.

    Science.gov (United States)

    van Asten, Liselotte; van der Lubben, Mariken; van den Wijngaard, Cees; van Pelt, Wilfrid; Verheij, Robert; Jacobi, Andre; Overduin, Pieter; Meijer, Adam; Luijt, Dirk; Claas, Eric; Hermans, Mirjam; Melchers, Willem; Rossen, John; Schuurman, Rob; Wolffs, Petra; Boucher, Charles; Bouchier, Charles; Schirm, Jurjen; Kroes, Louis; Leenders, Sander; Galama, Joep; Peeters, Marcel; van Loon, Anton; Stobberingh, Ellen; Schutten, Martin; Koopmans, Marion

    2009-07-01

    Experience with a highly pathogenic avian influenza outbreak in the Netherlands (2003) illustrated that the diagnostic demand for respiratory viruses at different biosafety levels (including BSL3), can increase unexpectedly and dramatically. We describe the measures taken since, aimed at strengthening national laboratory surge capacity and improving preparedness for dealing with diagnostic demand during outbreaks of (emerging) respiratory virus infections, including pandemic influenza virus. Academic and peripheral medical-microbiological laboratories collaborated to determine minimal laboratory requirements for the identification of viruses in the early stages of a pandemic or a large outbreak of avian influenza virus. Next, an enhanced collaborative national network of outbreak assistance laboratories (OAL) was set up. An inventory was made of the maximum diagnostic throughput that this network can deliver in a period of intensified demand. For an estimate of the potential magnitude of this surge demand, historical counts were calculated from hospital- and physician-based registries of patients presenting with respiratory symptoms. Number of respiratory physician-visits ranged from 140,000 to 615,000 per month and hospitalizations ranged from 3000 to 11,500 per month. The established OAL-network provides rapid diagnostic response with agreed quality requirements and a maximum throughput capacity of 1275 samples/day (38,000 per month), assuming other routine diagnostic work needs to be maintained. Thus surge demand for diagnostics for hospitalized cases (if not distinguishable from other respiratory illness) could be handled by the OAL network. Assessing etiology of community acquired acute respiratory infection however, may rapidly exceed the capacity of the network. Therefore algorithms are needed for triaging for laboratory diagnostics; currently this is not addressed in pandemic preparedness plans.

  9. The influence of the Cartagena Protocol on Biosafety: Comparing Mexico, China and South Africa..

    NARCIS (Netherlands)

    Gupta, A.; Falkner, R.

    2006-01-01

    This paper analyzes how the Cartagena Protocol on Biosafety, a global regime governing trade in genetically modified organisms (GMOs), is influencing agricultural biotechnology policy choices in developing countries/emerging economies. Through empirical analysis of Mexico, China and South Africa, we

  10. 42 CFR 9.10 - Occupational Health and Safety Program (OHSP) and biosafety requirements.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Occupational Health and Safety Program (OHSP) and... SANCTUARY SYSTEM § 9.10 Occupational Health and Safety Program (OHSP) and biosafety requirements. (a) How are employee Occupational Health and Safety Program risks and concerns addressed? The sanctuary shall...

  11. Public submissions on the Uganda national biotechnology and biosafety bill, 2012 reveal consensus for Uganda legislators to pass the bill

    Directory of Open Access Journals (Sweden)

    Clet Wandui Masiga

    2015-10-01

    Full Text Available The Cartagena Protocol on Biosafety to the Convention on Biological Diversity is an internationally binding instrument addressing issues of biosafety. Biosafety refers to the need to protect human health and the environment from the possible adverse effects of the products of modern biotechnology. Accordingly all countries to the convention are required to put in place regulatory mechanisms to enhance the safety of biotechnology in the context of the Convention’s overall goal of reducing all potential threats to biological diversity, while taking into account the risks to human health. Therefore each country party to the convention has its own procedures to enact laws to guide the safe use of biotechnology. In Uganda the process involves the drafting of the bill by the first parliamentary counsel, approval by cabinet, first reading at the parliament, committal to the responsible parliamentary sessional committee, tabling of the bill for public hearing, consultations, and final approval. In Uganda, the Committee on Science and Technology is responsible for the Biosafety Bill. In March 2013, the Committee tabled the bill for public hearing and submissions from public institutions. There were comments supporting the passage of the Bill and comments in objection.The reasons for objection are mainly due to precaution, speculation, lack of knowledge about biotechnology and biosafety, and alleged influence from biosafety entrepreneurs. This article reviews these public views, revealing controversy and possible consensus to pass the bill.

  12. Lessons to be Learned from Recent Biosafety Incidents in the United States.

    Science.gov (United States)

    Weiss, Shay; Yitzhaki, Shmuel; Shapira, Shmuel C

    2015-05-01

    During recent months, the Centers for Disease Control and Prevention (CDC) announced the occurrence of three major biosafety incidents, raising serious concern about biosafety and biosecurity guideline implementation in the most prestigious agencies in the United States: the CDC, the National Institutes of Health (NIH) and the Federal Drug Administration (FDA). These lapses included: a) the mishandling of Bacillus anthracis spores potentially exposing dozens of employees to anthrax; b) the shipment of low pathogenic influenza virus unknowingly cross-contaminated with a highly pathogenic strain; and c) an inventory lapse of hundreds of samples of biological agents, including six vials of variola virus kept in a cold storage room for decades, unnoticed. In this review we present the published data on these events, report the CDC inquiry's main findings, and discuss the key lessons to be learnt to ensure safer scientific practice in biomedical and microbiological service and research laboratories.

  13. Estimates and implications of the costs of compliance with biosafety regulations in developing countries.

    Science.gov (United States)

    Falck-Zepeda, Jose; Yorobe, Jose; Husin, Bahagiawati Amir; Manalo, Abraham; Lokollo, Erna; Ramon, Godfrey; Zambrano, Patricia; Sutrisno

    2012-01-01

    Estimating the cost of compliance with biosafety regulations is important as it helps developers focus their investments in producer development. We provide estimates for the cost of compliance for a set of technologies in Indonesia, the Philippines and other countries. These costs vary from US $100,000 to 1.7 million. These are estimates of regulatory costs and do not include product development or deployment costs. Cost estimates need to be compared with potential gains when the technology is introduced in these countries and the gains in knowledge accumulate during the biosafety assessment process. Although the cost of compliance is important, time delays and uncertainty are even more important and may have an adverse impact on innovations reaching farmers.

  14. Biosafety assessment protocols for new organisms in New Zealand: Can they apply internationally to emerging technologies?

    International Nuclear Information System (INIS)

    Barratt, B.I.P.; Moeed, A.; Malone, L.A.

    2006-01-01

    An analysis of established biosafety protocols for release into the environment of exotic plants and biological control agents for weeds and arthropod pests has been carried out to determine whether such protocols can be applied to relatively new and emerging technologies intended for the primary production industries, such as transgenic plants. Example case studies are described to indicate the scope of issues considered by regulators who make decisions on new organism releases. No transgenic plants have been released to date in New Zealand, but two field test approvals are described as examples. An analysis of the biosafety protocols has shown that, while many of the risk criteria considered for decision-making by regulators are similar for all new organisms, a case-by-case examination of risks and potential impacts is required in order to fully assess risk. The value of post-release monitoring and validation of decisions made by regulators is emphasised

  15. Biosafety decisions and perceived commercial risks: The role of GM-free private standards

    OpenAIRE

    Gruère, Guillaume; Sengupta, Debdatta

    2009-01-01

    "We herein investigate the observed discrepancy between real and perceived commercial risks associated with the use of genetically modified (GM) products in developing countries. We focus particularly on the effects of GM-free private standards set up by food companies in Europe and other countries on biotechnology and biosafety policy decisions in food-exporting developing countries. Based on field visits made to South Africa, Namibia, and Kenya in June 2007, and secondary information from t...

  16. Biosafety preclinical trials of xenogenic, Allogeniec and autologous progenitor cells from fat tissue

    OpenAIRE

    Melikhova, Vs; Saburina, I.; Repin, V.; Novikova, N.; Murashev, A.; Orlov, A.

    2008-01-01

    Due to wide spread of new methods of regenerative medicine on basis of stem cells it has become increasingly necessary to establish standards of techniques using them. Preclinical trial of a new technology after receiving preliminary experiment results is a primary stage of its adoption. We carried out the experiments to assess biosafety of autogenous, allergenic and xenogeneic cultures of human and rat cells in models under standard preclinical experiments conditions used in a presentday pha...

  17. Biosafety: degree of importance in the point of view of undergraduate dental students from Univille

    OpenAIRE

    Maria Dalva de S. SCHROEDER; Constanza MARIN; Fabio MIRI

    2010-01-01

    Introduction and objective: This study evaluated the degree ofimportance regarding biosafety in the point of view of undergraduatedental students from the University of the Region of Joinville– Univille. Material and methods: The sample was composed of142 undergraduate dental students from first to fifth year, who wereasked to sign the term of free and clarified assent in order to answera questionnaire with 13 closed-ended and 2 open-ended questionsregarding dental clinical practice and conce...

  18. Biosafety considerations for selectable and scorable markers used in cassava (Manihot esculenta Crantz) biotechnology.

    Science.gov (United States)

    Petersen, William; Umbeck, Paul; Hokanson, Karen; Halsey, Mark

    2005-01-01

    Cassava is an important subsistence crop grown only in the tropics, and represents a major source of calories for many people in developing countries. Improvements in the areas of resistance to insects and viral diseases, enhanced nutritional qualities, reduced cyanogenic content and modified starch characteristics are urgently needed. Traditional breeding is hampered by the nature of the crop, which has a high degree of heterozygosity, irregular flowering, and poor seed set. Biotechnology has the potential to enhance crop improvement efforts, and genetic engineering techniques for cassava have thus been developed over the past decade. Selectable and scorable markers are critical to efficient transformation technology, and must be evaluated for biosafety, as well as efficiency and cost-effectiveness. In order to facilitate research planning and regulatory submission, the literature on biosafety aspects of the selectable and scorable markers currently used in cassava biotechnology is surveyed. The source, mode of action and current use of each marker gene is described. The potential for toxicity, allergenicity, pleiotropic effects, horizontal gene transfer, and the impact of these on food or feed safety and environmental safety is evaluated. Based on extensive information, the selectable marker genes nptII, hpt, bar/pat, and manA, and the scorable marker gene uidA, all have little risk in terms of biosafety. These appear to represent the safest options for use in cassava biotechnology available at this time.

  19. State-of-the-art in biosafety and biosecurity in European countries.

    Science.gov (United States)

    Bielecka, Anna; Mohammadi, Ali Akbar

    2014-06-01

    The terms biosafety and biosecurity are widely used in different concepts and refer not only to protection of human beings and their surrounding environment against hazardous biological agent, but also to global disarmament of weapons of mass destruction. As a result, the biosafety and biosecurity issues should be considered interdisciplinary based on multilateral agreements against proliferation of biological weapons, public health and environmental protection. This publication presents information on both, international and national biosafety and biosecurity legislation. Status of national implementation of the Biological and Toxin Weapons Convention, penalization issues and measures to account for and secure production, use, storage of particularly dangerous pathogens or activities involving humans, plants and animals where infection may pose a risk have been analyzed. Safety and security measures in laboratories have been studied. Moreover, dual-use technology and measures of secure transport of biohazard materials have been also taken into account. In addition, genetic engineering regulations, biosecurity activities in laboratories and code of conducts have been investigated, as well.

  20. Genomic misconception: a fresh look at the biosafety of transgenic and conventional crops. A plea for a process agnostic regulation.

    Science.gov (United States)

    Ammann, Klaus

    2014-01-25

    The regulation of genetically engineered crops, in Europe and within the legislation of the Cartagena biosafety protocol is built on false premises: The claim was (and unfortunately still is) that there is a basic difference between conventional and transgenic crops, this despite the fact that this has been rejected on scientifically solid grounds since many years. This contribution collects some major arguments for a fresh look at regulation of transgenic crops, they are in their molecular processes of creation not basically different from conventional crops, which are based in their breeding methods on natural, sometimes enhanced mutation. But the fascination and euphoria of the discoveries in molecular biology and the new perspectives in plant breeding in the sixties and seventies led to the wrong focus on transgenic plants alone. In a collective framing process the initial biosafety debates focused on the novelty of the process of transgenesis. When early debates on the risk assessment merged into legislative decisions, this wrong focus on transgenesis alone seemed uncontested. The process-focused view was also fostered by a conglomerate of concerned scientists and biotechnology companies, both with a vested interest to at least tolerate the rise of the safety threshold to secure research money and to discourage competitors of all kinds. Policy minded people and opponent activists without deeper insight in the molecular science agreed to those efforts without much resistance. It is interesting to realize, that the focus on processes was uncontested by a majority of regulators, this despite of serious early warnings from important authorities in science, mainly of US origin. It is time to change the regulation of genetically modified (GM) crops toward a more science based process-agnostic legislation. Although this article concentrates on the critique of the process-oriented regulation, including some details about the history behind, there should be no

  1. Biological Terrorism: US Policies to Reduce Global Biothreats

    Science.gov (United States)

    2008-09-01

    program for pro- jects that advance BEP objectives. Global Cooperation to develop bio- safety and pathogen security stan- dards that are consistent with...security. The Organization for Economic Cooperation and Development ( OECD ) has recently developed voluntary biosecurity guidelines for implementation...Abbreviations AG Australia Group BEP Biosecurity Engagement Program BSL Biosafety level BWC Biological Weapons Convention BWC-ISU Biological Weapons

  2. A universal next generation sequencing protocol to generate non-infectious barcoded cDNA libraries from high containment RNA viruses

    Science.gov (United States)

    Several biosafety level (BSL)-3/4 pathogens are high consequence, single-stranded RNA viruses and their genomes, when introduced into permissive cells, are infectious. Moreover many of these viruses are Select Agents (SAs), and their genomes are also considered SAs. For this reason cDNAs and/or th...

  3. Clinical Characterization of Aerosolized Francisella tularensis Infection in Cynomolgus macaques

    Science.gov (United States)

    2016-11-21

    All airflows, environmental monitoring, and system balancing were configured, controlled, and recorded through the institute standard automated...were used as a baseline to fit an autoregressive integrated moving average (ARIMA) model. Temperature elevations exceeding three standard deviations...supervision of a pathologist board certified by the American College of Veterinary Pathologist in a biosafety level (BSL) 3 necropsy facility. Tissues

  4. A Curriculum-Based Approach to Teaching Biosafety Through eLearning.

    Science.gov (United States)

    Ndolo, Dennis O; Wach, Michael; Rüdelsheim, Patrick; Craig, Wendy

    2018-01-01

    Anyone working in biosafety capacity enhancement faces the challenge of ensuring that the impact of a capacity enhancing activity continues and becomes sustainable beyond the depletion of funding. Many training efforts face the limitation of one-off events: they only reach those people present at the time. It becomes incumbent upon the trainees to pass on the training to colleagues as best they can, whilst the demand for the training never appears to diminish. However, beyond the initial effort to establish the basic content, repeating capacity enhancement events in different locations is usually not economically feasible. Also, the lack of infrastructure and other resources needed to support a robust training programme hinder operationalizing a "train-the-trainer" approach to biosafety training. One way to address these challenges is through the use of eLearning modules that can be delivered online, globally, continuously, at low cost, and on an as-needed basis to multiple audiences. Once the modules are developed and peer-reviewed, they can be maintained on a remote server and made available to various audiences through a password-protected portal that delivers the programme content, administers preliminary and final exams, and provides the administrative infrastructure to register users and track their progress through the modules. Crucial to the implementation of such an eLearning programme is an approach in which the modules are intentionally developed together as a cohesive curriculum. Once developed, such a curriculum can be released as a stand-alone programme for the training of governmental risk assessors and regulators or used as accredited components in post-graduate degree programmes in biosafety, at minimal cost to the government or university. Examples from the portfolio of eLearning modules developed by the International Centre for Genetic Engineering and Biotechnology (ICGEB) are provided to demonstrate these key features.

  5. Surface-Layer (S-Layer) Proteins Sap and EA1 Govern the Binding of the S-Layer-Associated Protein BslO at the Cell Septa of Bacillus anthracis

    Science.gov (United States)

    Kern, Valerie J.; Kern, Justin W.; Theriot, Julie A.; Schneewind, Olaf

    2012-01-01

    The Gram-positive pathogen Bacillus anthracis contains 24 genes whose products harbor the structurally conserved surface-layer (S-layer) homology (SLH) domain. Proteins endowed with the SLH domain associate with the secondary cell wall polysaccharide (SCWP) following secretion. Two such proteins, Sap and EA1, have the unique ability to self-assemble into a paracrystalline layer on the surface of bacilli and form S layers. Other SLH domain proteins can also be found within the S layer and have been designated Bacillus S-layer-associated protein (BSLs). While both S-layer proteins and BSLs bind the same SCWP, their deposition on the cell surface is not random. For example, BslO is targeted to septal peptidoglycan zones, where it catalyzes the separation of daughter cells. Here we show that an insertional lesion in the sap structural gene results in elongated chains of bacilli, as observed with a bslO mutant. The chain length of the sap mutant can be reduced by the addition of purified BslO in the culture medium. This complementation in trans can be explained by an increased deposition of BslO onto the surface of sap mutant bacilli that extends beyond chain septa. Using fluorescence microscopy, we observed that the Sap S layer does not overlap the EA1 S layer and slowly yields to the EA1 S layer in a growth-phase-dependent manner. Although present all over bacilli, Sap S-layer patches are not observed at septa. Thus, we propose that the dynamic Sap/EA1 S-layer coverage of the envelope restricts the deposition of BslO to the SCWP at septal rings. PMID:22609927

  6. New International Initiatives on Enhancement of Biosafety and Biosecurity Regulations for Laboratories Handling Infectious Agents

    International Nuclear Information System (INIS)

    Netesov, S. V.; Drozdov, I. G.

    2007-01-01

    Before we entered the era of antibiotics, development of antiseptics rules and reliable water purification systems the infectious pathogens had played a major role in morbidity and mortality of global human population. The advances in revealing the nature of dangerous infections and studying their causative agents during the recent years have led not only to big progress in their control but also to the study of their potential as weapons. During the last fifty years, several attempts have been made to use them for criminal or terrorist purposes that demonstrated that even primitively organized terrorist attacks may lead to quite significant consequences. The October 2001 events showed that bioterrorism attacks may be prepared, probably, as a result of theft of the pathogen from a lab. All this led to the revision and radical improvement of current national rules and international recommendations in the field of handling, storage and transportation of infectious agents. As a result, during the past two years these rules have been significantly revised by both the World Health Organization and some countries. However, their harmonization of is still far from what is desired. Therefore, biosafety professionals in some countries, including those of the European Union, are establishing professional biosafety associations. In addition, new initiatives are being proposed to develop internationally harmonized biosecurity rules to govern dangerous pathogens handling and storage. The most important of them are as follows: 1. Development, under the auspices of WHO, of new recommendations concerning a set of requirements to provide physical security of both biological agents and laboratories involved in research on extremely hazardous infections; 2. Enhacement, under the auspices of WHO, of current international recommendations on inventory procedures and regulations, inventory monitoring, and transportation of specimens and strains of extremely hazardous infections; 3

  7. Analysis of the economic impact of environmental biosafety works projects in healthcare centres in extremadura (spain)

    OpenAIRE

    García Sanz-Calcedo, Justo; Monzón-González, Pedro

    2014-01-01

    The aim of this paper is to analyze the results obtained in the methodological application of techniques aimed at the maintenance of environmental biosafety in works of reform and expansion of healthcare centres in Extremadura, Spain during 2004-2010, assessing the costs of its implementation and contrasting if the use of a BSA project in phase of works affects the probability of nosocomial infection and the conditions of health and safety. The average investment accounted for a cost of 5.5 €...

  8. Biosafety considerations of RNAi-mediated virus resistance in fruit-tree cultivars and in rootstock.

    Science.gov (United States)

    Lemgo, Godwin Nana Yaw; Sabbadini, Silvia; Pandolfini, Tiziana; Mezzetti, Bruno

    2013-12-01

    A major application of RNA interference (RNAi) is envisaged for the production of virus-resistant transgenic plants. For fruit trees, this remains the most, if not the only, viable option for the control of plant viral disease outbreaks in cultivated orchards, due to the difficulties associated with the use of traditional and conventional disease-control measures. The use of RNAi might provide an additional benefit for woody crops if silenced rootstock can efficiently transmit the silencing signal to non-transformed scions, as has already been demonstrated in herbaceous plants. This would provide a great opportunity to produce non-transgenic fruit from transgenic rootstock. In this review, we scrutinise some of the concerns that might arise with the use of RNAi for engineering virus-resistant plants, and we speculate that this virus resistance has fewer biosafety concerns. This is mainly because RNAi-eliciting constructs only express small RNA molecules rather than proteins, and because this technology can be applied using plant rootstock that can confer virus resistance to the scion, leaving the scion untransformed. We discuss the main biosafety concerns related to the release of new types of virus-resistant plants and the risk assessment approaches in the application of existing regulatory systems (in particular, those of the European Union, the USA, and Canada) for the evaluation and approval of RNAi-mediated virus-resistant plants, either as transgenic varieties or as plant virus resistance induced by transgenic rootstock.

  9. Mesoporous Silica and Organosilica Nanoparticles: Physical Chemistry, Biosafety, Delivery Strategies, and Biomedical Applications

    KAUST Repository

    Croissant, Jonas G.

    2017-11-30

    Predetermining the physico-chemical properties, biosafety, and stimuli-responsiveness of nanomaterials in biological environments is essential for safe and effective biomedical applications. At the forefront of biomedical research, mesoporous silica nanoparticles and mesoporous organosilica nanoparticles are increasingly investigated to predict their biological outcome by materials design. In this review, it is first chronicled that how the nanomaterial design of pure silica, partially hybridized organosilica, and fully hybridized organosilica (periodic mesoporous organosilicas) governs not only the physico-chemical properties but also the biosafety of the nanoparticles. The impact of the hybridization on the biocompatibility, protein corona, biodistribution, biodegradability, and clearance of the silica-based particles is described. Then, the influence of the surface engineering, the framework hybridization, as well as the morphology of the particles, on the ability to load and controllably deliver drugs under internal biological stimuli (e.g., pH, redox, enzymes) and external noninvasive stimuli (e.g., light, magnetic, ultrasound) are presented. To conclude, trends in the biomedical applications of silica and organosilica nanovectors are delineated, such as unconventional bioimaging techniques, large cargo delivery, combination therapy, gaseous molecule delivery, antimicrobial protection, and Alzheimer\\'s disease therapy.

  10. International Society for the Advancement of Cytometry cell sorter biosafety standards.

    Science.gov (United States)

    Holmes, Kevin L; Fontes, Benjamin; Hogarth, Philip; Konz, Richard; Monard, Simon; Pletcher, Charles H; Wadley, Robert B; Schmid, Ingrid; Perfetto, Stephen P

    2014-05-01

    Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument operators and the environment from biohazards arising from the use of sorters. To this end the International Society of Analytical Cytology (ISAC) was proactive in establishing biosafety guidelines in 1997 (Schmid et al., Cytometry 1997;28:99-117) and subsequently published revised biosafety standards for cell sorting of unfixed samples in 2007 (Schmid et al., Cytometry Part A J Int Soc Anal Cytol 2007;71A:414-437). Since their publication, these documents have become recognized worldwide as the standard of practice and safety precautions for laboratories performing cell sorting experiments. However, the field of cytometry has progressed since 2007, and the document requires an update. The new Standards provides guidance: (1) for laboratory design for cell sorter laboratories; (2) for the creation of laboratory or instrument specific Standard Operating Procedures (SOP); and (3) on procedures for the safe operation of cell sorters, including personal protective equipment (PPE) and validation of aerosol containment. Published © 2014 Wiley Periodicals Inc.

  11. Biosafety assessment of transgenic Bt cotton on model animals

    Directory of Open Access Journals (Sweden)

    Sadia Bano

    2016-05-01

    Full Text Available Abstract Background: To know the effects of transgenic crops on soil microorganisms, animals and other expected hazards due to the introduction of GM crops into the environment is critical both scientifically and environmentally. The work was conducted to study the effect of insecticidal Bt protein on Rats and Earthworms. Methods: For this purpose, animals like rat and soil organisms like Earthworm were selected. Rats were selected on the basis of its 95% homology on genomic, cellular and enzymatic level with human while earthworm were preferred on the basis of their direct contact with soil to evaluate the impact of Bt (Cry1AC crop field soil on earthworm, secreted by root exudates of Bt cotton. Several physical, molecular, biochemical and histological analyses were performed on both Rats/Earthworms fed on standard diet (control group as well containing Bt protein (experimental group. Results: Molecular analyses such as immune Dot blot, SDS-PAGE, ELISA and PCR, confirmed the absence of Cry1Ac protein in blood and urine samples of rats, which were fed with Bt protein in their diet. Furthermore, histological studies showed that there was no difference in cellular architecture in liver, heart, kidney and intestine of Bt and non-Bt diet fed rats. To see the effect of Bt on earthworm two different groups were studied, one with transgenic plant field soil supplemented with grinded leaves of cotton and second group with non-Bt field soil. Conclusions: No lethal effects of transgenic Bt protein on the survival of earthworm and rats were observed. Bradford assay, Dipstick assay ELISA demonstrated the absence of Cry1Ac protein in the mid-gut epithelial tissue of earthworm. The results of present study will be helpful in successful deployment and commercial release of genetically modified crop in Pakistan.

  12. Case studies on the use of biotechnologies and on biosafety provisions in four African countries.

    Science.gov (United States)

    Black, Robert; Fava, Fabio; Mattei, Niccolo; Robert, Vincent; Seal, Susan; Verdier, Valerie

    2011-12-20

    This review is based on a study commissioned by the European Commission on the evaluation of scientific, technical and institutional challenges, priorities and bottlenecks for biotechnologies and regional harmonisation of biosafety in Africa. Biotechnology was considered within four domains: agricultural biotechnologies ('Green'), industrial biotechnologies and biotechnologies for environmental remediation ('White'), biotechnologies in aquaculture ('Blue') and biotechnologies for healthcare ('Red'). An important consideration was the decline in partnerships between the EU and developing countries because of the original public antipathy to some green biotechnologies, particularly genetically modified organisms (GMOs) and food from GM crops in Europe. The study focus reported here was West Africa (Ghana, Senegal, Mali and Burkina Faso). The overall conclusion was that whereas high-quality research was proceeding in the countries visited, funding is not sustained and there is little evidence of practical application of biotechnology and benefit to farmers and the wider community. Research and development that was being carried out on genetically modified crop varieties was concentrating on improving food security and therefore unlikely to have significant impact on EU markets and consumers. However, there is much non-controversial green biotechnology such as molecular diagnostics for plant and animal disease and marker-assisted selection for breeding that has great potential application. Regarding white biotechnology, it is currently occupying only a very small industrial niche in West Africa, basically in the sole sector of the production of liquid biofuels (i.e., bio-ethanol) from indigenous and locally planted biomass (very often non-food crops). The presence of diffused small-scale fish production is the basis to develop and apply new (Blue) aquaculture technologies and, where the research conditions and the production sector can permit, to increase this type of

  13. Development of an eco-friendly mosquitocidal agent from Alangium salvifolium against the dengue vector Aedes aegypti and its biosafety on the aquatic predator.

    Science.gov (United States)

    Thanigaivel, Annamalai; Vasantha-Srinivasan, Prabhakaran; Edwin, Edward-Sam; Ponsankar, Athirstam; Selin-Rani, Selvaraj; Chellappandian, Muthiah; Kalaivani, Kandaswamy; Senthil-Nathan, Sengottayan; Benelli, Giovanni

    2018-04-01

    Plant extracts with their enriched chemical constituents have established potential alternative mosquito control agents. In this research, we developed an eco-friendly mosquitocidal agent from Alangium salvifolium leaves against the dengue and Zika virus vector Aedes aegypti and we investigated its biosafety on the mosquito aquatic predator Toxorhynchites splendens. Results showed that the methanolic extract of A. salvifolium leaves was composed by eight main compounds, with major peak area for hexadecenoic acid (21.74%). LC 50 and LC 90 values calculated on Ae. aegypti fourth instar larvae were 104.80 and 269.15 ppm respectively. The methanolic extract tested at 100 ppm decreased the α-β carboxylesterase and SOD ratio significantly and upregulated the GST and CYP450 level. The A. salvifolium methanolic extract displayed significant repellent and adulticidal activity at 100 and 400 ppm respectively. The treatment with 100 ppm of the methanolic extract led to 210 min of protection from Ae. aegypti bites. Four hundred parts per million of the extract showed 98% adult mortality within 30 min from the treatment. Lastly, biosafety assays on the mosquito aquatic predator Tx. splendens showed that the toxicity of the A. salvifolium extract was significantly lower if compared to the cypermethrin-based treatments. The methanolic extract of A. salvifolium showed a maximum of 47.3% mortality rate at the concentration of 1000 ppm, while 0.7 ppm of cypermethrin achieved 91.3% mortality rate on Tx. splendens. Overall, our study enhances basic knowledge on how to improve natural larvicidal agents against dengue and Zika virus mosquito vector with harmless responses on non-target aquatic predators.

  14. biosafety regulations

    African Journals Online (AJOL)

    Modern biotechnology will provide important tools for developing countries seeking to gain benefits from ..... several conferences in Latin America that have difficulties in successful transfer of the technology .... innovation in biotechnology.

  15. Factors influencing biosafety level and lai among the staff of medical laboratories

    Directory of Open Access Journals (Sweden)

    Anna Kozajda

    2013-08-01

    Full Text Available Background: The aim of the study was to assess the biological risks of medical laboratory employees with particular focus on laboratory acquired infection (LAI, activities having the greatest risk, accidents with biological material, post exposure procedure, preventive measures and workers' knowledge about biological exposure. Materials and Methods: The study involved 9 laboratories. A questionnaire survey was attended by 123 employees and 9 heads of these units with the use of two questionnaires for laboratory workers and the managers. Results: 32.5% of the respondents (40 persons had an accident at least once. Needlestick or a broken glass injury covered 18.7% respondents (23 persons, while splashing the skin, mucous membranes or conjunctivae related to 22.8% (28 persons. Among the employees who had an accident, only 45% of the respondents (18 persons reported this to the manager. Microbes dominant in the biological material were known only to 57 respondents (46.3%, less than half could correctly give an example of a disease (57 persons, 46.3%. More than half of the respondents admitted that they do not know all of the possible routes of infection while working in the laboratory (68 persons, 55.3%. Conclusions: In the study population, a high incidence of accidents was observed, usually during blood sampling and transfer of biological material. Condition of the workers' equipment with personal protective measures and laboratory facilities in devices to reduce the risk of infection and procedures for handling the potentially infectious material should be considered as insufficient. Lack of basic knowledge of the employees about biohazards at workplaces was shown. Med Pr 2013;64(4:473–486

  16. Factors influencing biosafety level and LAI among the staff of medical laboratories.

    Science.gov (United States)

    Kozajda, Anna; Bródka, Karolina; Szadkowska-Stańczyk, Irena

    2013-01-01

    The aim of the study was to assess the biological risks of medical laboratory employees with particular focus on laboratory acquired infection (LAI), activities having the greatest risk, accidents with biological material, post exposure procedure, preventive measures and workers' knowledge about biological exposure. The study involved 9 laboratories. A questionnaire survey was attended by 123 employees and 9 heads of these units with the use of two questionnaires for laboratory workers and the managers. 32.5% of the respondents (40 persons) had an accident at least once. Needlestick or a broken glass injury covered 18.7% respondents (23 persons), while splashing the skin, mucous membranes or conjunctivae related to 22.8% (28 persons). Among the employees who had an accident, only 45% of the respondents (18 persons) reported this to the manager. Microbes dominant in the biological material were known only to 57 respondents (46.3%), less than half could correctly give an example of a disease (57 persons, 46.3%). More than half of the respondents admitted that they do not know all of the possible routes of infection while working in the laboratory (68 persons, 55.3%). In the study population, a high incidence of accidents was observed, usually during blood sampling and transfer of biological material. Condition of the workers' equipment with personal protective measures and laboratory facilities in devices to reduce the risk of infection and procedures for handling the potentially infectious material should be considered as insufficient. Lack of basic knowledge of the employees about biohazards at workplaces was shown.

  17. Factors influencing biosafety level and lai among the staff of medical laboratories

    OpenAIRE

    Anna Kozajda; Karolina Bródka; Irena Szadkowska-Stańczyk

    2013-01-01

    Background: The aim of the study was to assess the biological risks of medical laboratory employees with particular focus on laboratory acquired infection (LAI), activities having the greatest risk, accidents with biological material, post exposure procedure, preventive measures and workers' knowledge about biological exposure. Materials and Methods: The study involved 9 laboratories. A questionnaire survey was attended by 123 employees and 9 heads of these units with the use of two questionn...

  18. Development of imaging techniques to study the pathogenesis of biosafety level 2/3 infectious agents.

    Science.gov (United States)

    Rella, Courtney E; Ruel, Nancy; Eugenin, Eliseo A

    2014-12-01

    Despite significant advances in microbiology and molecular biology over the last decades, several infectious diseases remain global concerns, resulting in the death of millions of people worldwide each year. According to the Center for Disease Control (CDC) in 2012, there were 34 million people infected with HIV, 8.7 million new cases of tuberculosis, 500 million cases of hepatitis, and 50-100 million people infected with dengue. Several of these pathogens, despite high incidence, do not have reliable clinical detection methods. New or improved protocols have been generated to enhance detection and quantitation of several pathogens using high-end microscopy (light, confocal, and STORM microscopy) and imaging software. In the current manuscript, we discuss these approaches and the theories behind these methodologies. Thus, advances in imaging techniques will open new possibilities to discover therapeutic interventions to reduce or eliminate the devastating consequences of infectious diseases. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  19. Newly developed chitosan-silver hybrid nanoparticles: biosafety and apoptosis induction in HepG2 cells

    International Nuclear Information System (INIS)

    El-Sherbiny, Ibrahim M.; Salih, Ehab; Yassin, Abdelrahman M.; Hafez, Elsayed E.

    2016-01-01

    The present study reports the biosafety assessment, the exact molecular effects, and apoptosis induction of newly developed chitosan-silver hybrid nanoparticles (Cs–Ag NPs) in HepG2 cells. The investigated hybrid NPs were green synthesized using Cs/grape leaves aqueous extract (Cs/GLE) or Cs/GLE NPs as reducing and stabilizing agents. The successful formation of Cs/GLE NPs and Cs–Ag hybrid NPs has been confirmed by UV–Vis spectrophotometry, FTIR spectroscopy, XRD, and HRTEM. From the TEM analysis, the prepared Cs/GLE NPs are uniform and spherical with an average size of 150 nm, and the AgNPs (5–10 nm) were formed mainly on their surface. The UV–Vis spectra of Cs–Ag NPs showed a surface plasmon resonance (SPR) peak at about 450 nm confirming their formation. The synthesized Cs–Ag NPs were found to be crystalline as shown by XRD patterns with fcc phase oriented along the (111), (200), (220), and (311) planes. The cytotoxicity patterns, the antiproliferative activities, and the possible mechanisms of anticancer activity at molecular level of the newly developed Cs–Ag hybrid NPs were investigated. Cytotoxicity patterns of all the preparations demonstrated that the nontoxic treatment concentrations are ranged from 0.39 to 50 %, and many of the newly prepared Cs–Ag hybrid NPs showed high anticancer activities against HpG2 cells, and induced cellular apoptosis by downregulating BCL2 gene and upregulating P53.Graphical Abstract

  20. Newly developed chitosan-silver hybrid nanoparticles: biosafety and apoptosis induction in HepG2 cells

    Energy Technology Data Exchange (ETDEWEB)

    El-Sherbiny, Ibrahim M., E-mail: ielsherbiny@Zewailcity.edu.eg; Salih, Ehab [Zewail City of Science and Technology, Center for Materials Science (Egypt); Yassin, Abdelrahman M. [Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications, Biopharmaceutical Product Research Department (Egypt); Hafez, Elsayed E. [City of Scientific Research and Technology Applications, Plant Protection and Biomolecular Diagnosis Department (Egypt)

    2016-07-15

    The present study reports the biosafety assessment, the exact molecular effects, and apoptosis induction of newly developed chitosan-silver hybrid nanoparticles (Cs–Ag NPs) in HepG2 cells. The investigated hybrid NPs were green synthesized using Cs/grape leaves aqueous extract (Cs/GLE) or Cs/GLE NPs as reducing and stabilizing agents. The successful formation of Cs/GLE NPs and Cs–Ag hybrid NPs has been confirmed by UV–Vis spectrophotometry, FTIR spectroscopy, XRD, and HRTEM. From the TEM analysis, the prepared Cs/GLE NPs are uniform and spherical with an average size of 150 nm, and the AgNPs (5–10 nm) were formed mainly on their surface. The UV–Vis spectra of Cs–Ag NPs showed a surface plasmon resonance (SPR) peak at about 450 nm confirming their formation. The synthesized Cs–Ag NPs were found to be crystalline as shown by XRD patterns with fcc phase oriented along the (111), (200), (220), and (311) planes. The cytotoxicity patterns, the antiproliferative activities, and the possible mechanisms of anticancer activity at molecular level of the newly developed Cs–Ag hybrid NPs were investigated. Cytotoxicity patterns of all the preparations demonstrated that the nontoxic treatment concentrations are ranged from 0.39 to 50 %, and many of the newly prepared Cs–Ag hybrid NPs showed high anticancer activities against HpG2 cells, and induced cellular apoptosis by downregulating BCL2 gene and upregulating P53.Graphical Abstract.

  1. Entomotoxicity and biosafety assessment of PEGylated acephate nanoparticles: a biologically safe alternative to neurotoxic pesticides.

    Science.gov (United States)

    Pradhan, Saheli; Roy, Indrani; Lodh, Gopal; Patra, Prasun; Choudhury, Samrat Roy; Samanta, Arunava; Goswami, Arunava

    2013-01-01

    This is a report of an experimental study on a nanoencapsulation of the organophosphate acephate. Acephate was encapsulated in polyethylene glycol, using a simple, easy-to-replicate method that required no special equipment or conditions. The nanoencapsulation (nanoacephate) was characterized and its bioefficacy as compared to the regular commercial acephate was tested. The biosafety of the new compound was also tested on a murine model. Our new nanoencapsulation scored over the regular variety on all counts. It was found to successfully incorporate the active pesticidal component, acephate and this compound retained greater functional integrity over time as a nanoencapsulation. It was significantly more efficacious than the regular variety. It was biosafe when tested on murine model. We have reason to believe that this nanoencapsulation would allow the use of an organophosphate in a more targeted manner, thereby making it a cost-effective and eco-friendly alternative to the regular variety in use now.

  2. Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations

    Directory of Open Access Journals (Sweden)

    Amaya Leunda

    2014-06-01

    Full Text Available Novel efficient vaccines are needed to control tuberculosis (TB, a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine. In this review we provide up to date information on novel tuberculosis (TB vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed.

  3. Green conversion of graphene oxide to graphene nanosheets and its biosafety study.

    Directory of Open Access Journals (Sweden)

    Adhiraj Dasgupta

    Full Text Available Chemical reduction of graphene oxide (GO to graphene employs the use of toxic and environmentally harmful reducing agents, hindering mass production of graphene which is of tremendous technological importance. In this study we report a green approach to the synthesis of graphene, bio-reduced by crude polysaccharide. The polysaccharide reduces exfoliated GO to graphene at room temperature in an aqueous medium. Transmission electron microscopy image provides clear evidence for the formation of few layer graphene. Characterization of the resulting polysaccharide reduced GO by Raman spectroscopy, Fourier transform infrared spectroscopy and Energy dispersive X-ray analysis confirms reduction of GO to graphene. We also investigated the degree of biosafety of the reduced GO and found it to be safe under 100 μg/ml.

  4. Evaluation on biosafety in long-term administration, teratogenicity and local toxicity of developed product

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sung-Ho; Kim, Jong-Chun; Kim, Se-Ra; Lee, Hae-Jun; Lee, Jin-Hee [Chonnam Nat. Univ., Gwangju (Korea, Republic of)

    2006-01-15

    We performed this study to determine biosafety of developed product in long-term administration and teratogenicity and local toxicity (skin and eye) of developed product (HemoHIM and HemoTonic). It is suggested that long-term administration with the developed products may not exert considerable side effects. It is concluded that the administration of HemoHIM or HemoTonic does not inflict any adverse effect on fetuses of pregnant mice. HemoHIM and HemoTonic could be considered as a no irritating materials to the skin and eye of the test animals. These results indicated that HemoHIM and HemoTonic might be a useful functional food, especially since it is a relatively nontoxic natural product.

  5. Evaluation on biosafety in long-term administration, teratogenicity and local toxicity of developed product

    International Nuclear Information System (INIS)

    Kim, Sung-Ho; Kim, Jong-Chun; Kim, Se-Ra; Lee, Hae-Jun; Lee, Jin-Hee

    2006-01-01

    We performed this study to determine biosafety of developed product in long-term administration and teratogenicity and local toxicity (skin and eye) of developed product (HemoHIM and HemoTonic). It is suggested that long-term administration with the developed products may not exert considerable side effects. It is concluded that the administration of HemoHIM or HemoTonic does not inflict any adverse effect on fetuses of pregnant mice. HemoHIM and HemoTonic could be considered as a no irritating materials to the skin and eye of the test animals. These results indicated that HemoHIM and HemoTonic might be a useful functional food, especially since it is a relatively nontoxic natural product

  6. Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations.

    Science.gov (United States)

    Leunda, Amaya; Baldo, Aline; Goossens, Martine; Huygen, Kris; Herman, Philippe; Romano, Marta

    2014-06-16

    Novel efficient vaccines are needed to control tuberculosis (TB), a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG) to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine). In this review we provide up to date information on novel tuberculosis (TB) vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO) which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed.

  7. Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations

    Science.gov (United States)

    Leunda, Amaya; Baldo, Aline; Goossens, Martine; Huygen, Kris; Herman, Philippe; Romano, Marta

    2014-01-01

    Novel efficient vaccines are needed to control tuberculosis (TB), a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG) to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine). In this review we provide up to date information on novel tuberculosis (TB) vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO) which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed. PMID:26344627

  8. South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory.

    Directory of Open Access Journals (Sweden)

    Janusz T Paweska

    2017-06-01

    Full Text Available In August 2014, the National Institute for Communicable Diseases (NICD in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD cases.The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone. Furthermore, the NICD set out to establish local capacity amongst Sierra Leonean nationals in all aspects of the FEDL functions from the outset. This led to the successful hand-over of the FEDL to the Sierra Leone Ministry of Health and Sanitation in March 2015. Between 25 August 2014 and 22 June 2016, the laboratory tested 11,250 specimens mostly from the Western Urban and Western Rural regions of Sierra Leone, of which 2,379 (21.14% tested positive for Ebola virus RNA.The bio-safety standards and the portability of the SA FEDL, offered a cost-effective and practical alternative for the rapid deployment of a field-operated high biocontainment facility. The SA FEDL teams demonstrated that it is highly beneficial to train the national staff in the course of formidable disease outbreak and accomplished their full integration into all operational and diagnostic aspects of the laboratory. This initiative contributed to the international efforts in bringing the EVD outbreak under control in Sierra Leone, as well as capacitating local African scientists and technologists to respond to diagnostic needs that might be required in future outbreaks of highly contagious pathogens.

  9. South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory.

    Science.gov (United States)

    Paweska, Janusz T; Jansen van Vuren, Petrus; Meier, Gunther H; le Roux, Chantel; Conteh, Ousman S; Kemp, Alan; Fourie, Cardia; Naidoo, Prabha; Naicker, Serisha; Ohaebosim, Phumza; Storm, Nadia; Hellferscee, Orienka; Ming Sun, Lisa K; Mogodi, Busisiwe; Prabdial-Sing, Nishi; du Plessis, Desiree; Greyling, Deidre; Loubser, Shayne; Goosen, Mark; McCulloch, Stewart D; Scott, Terence P; Moerdyk, Alexandra; Dlamini, Wesley; Konneh, Kelfala; Kamara, Idrissa L; Sowa, Dauda; Sorie, Samuel; Kargbo, Brima; Madhi, Shabir A

    2017-06-01

    In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone. Furthermore, the NICD set out to establish local capacity amongst Sierra Leonean nationals in all aspects of the FEDL functions from the outset. This led to the successful hand-over of the FEDL to the Sierra Leone Ministry of Health and Sanitation in March 2015. Between 25 August 2014 and 22 June 2016, the laboratory tested 11,250 specimens mostly from the Western Urban and Western Rural regions of Sierra Leone, of which 2,379 (21.14%) tested positive for Ebola virus RNA. The bio-safety standards and the portability of the SA FEDL, offered a cost-effective and practical alternative for the rapid deployment of a field-operated high biocontainment facility. The SA FEDL teams demonstrated that it is highly beneficial to train the national staff in the course of formidable disease outbreak and accomplished their full integration into all operational and diagnostic aspects of the laboratory. This initiative contributed to the international efforts in bringing the EVD outbreak under control in Sierra Leone, as well as capacitating local African scientists and technologists to respond to diagnostic needs that might be required in future outbreaks of highly contagious pathogens.

  10. Biosafety management and commercial use of genetically modified crops in China.

    Science.gov (United States)

    Li, Yunhe; Peng, Yufa; Hallerman, Eric M; Wu, Kongming

    2014-04-01

    As a developing country with relatively limited arable land, China is making great efforts for development and use of genetically modified (GM) crops to boost agricultural productivity. Many GM crop varieties have been developed in China in recent years; in particular, China is playing a leading role in development of insect-resistant GM rice lines. To ensure the safe use of GM crops, biosafety risk assessments are required as an important part of the regulatory oversight of such products. With over 20 years of nationwide promotion of agricultural biotechnology, a relatively well-developed regulatory system for risk assessment and management of GM plants has been developed that establishes a firm basis for safe use of GM crops. So far, a total of seven GM crops involving ten events have been approved for commercial planting, and 5 GM crops with a total of 37 events have been approved for import as processing material in China. However, currently only insect-resistant Bt cotton and disease-resistant papaya have been commercially planted on a large scale. The planting of Bt cotton and disease-resistant papaya have provided efficient protection against cotton bollworms and Papaya ringspot virus (PRSV), respectively. As a consequence, chemical application to these crops has been significantly reduced, enhancing farm income while reducing human and non-target organism exposure to toxic chemicals. This article provides useful information for the colleagues, in particular for them whose mother tongue is not Chinese, to clearly understand the biosafety regulation and commercial use of genetically modified crops in China.

  11. Screening of Indian medicinal plants for cytotoxic activity by Brine Shrimp Lethality (BSL assay and evaluation of their total phenolic content

    Directory of Open Access Journals (Sweden)

    Mahesh Biradi

    2014-01-01

    Full Text Available Objective: Plant-derived cytotoxic constituents and polyphenolic compounds have played an important role in the development of clinically useful anticancer agents. In this context, we have selected six Indian medicinal plants based on the literature claims and an attempt was made to evaluate the cytotoxic potential and total phenolic content (TPC of their methanol extracts and fractions. Materials and Methods: Six plants have been selected for the study, namely, Artemisia absinthium Linn. (Asteraceae, Oroxylum indicum (Linn. Vent. (Bignoniaceae, Heliotropium indicum Linn. (Boraginaceae, Amorphophallus sylvaticus (Roxb. Kunth. (Araceae, Mimosa pudica Linn. (Mimosaceae, and Premna serratifolia Linn. (Verbenaceae. Authenticated plant materials were subjected to extraction with methanol by cold maceration and hot percolation methods. The extracts were fractionated into four fractions (F1, F2, F3, and F4. Preliminary phytochemical investigation was carried out for all extracts and fractions. All extracts and their fractions were subjected to cytotoxicity screening by brine shrimp lethality (BSL bioassay. The plants with significant cytotoxicity were evaluated for TPC by using Folin-Ciocalteu reagent. Results: F1, F2, and F3 fractions of A. absinthium and P. serratifolia and F1 fraction of M. pudica have shown significant cytotoxicity (lethal concentration (LC 50 < 100 ppm compared with other fractions. F1, F2, and F3 fractions of A. absinthium show the LC 50 values 32.52, 14.27, and 24.02, respectively; F1, F2, and F3 of P. serratifolia show LC 50 values 7.61, 4.01, and 10.91 and same for F1 fraction of M. pudica was 34.82 μg/ml, respectively. TPC was found to be significantly higher (39.11 mg gallic acid equivalent (GAE/g in P. serratifolia compared with other two plants. Conclusion: The cytotoxicity screening system confirmed the proposed anticancer plants used by traditional healers and literature claims.

  12. On the interpretation of millennium-scale level variations of the Black Sea during the first quarter of the Holocene

    Science.gov (United States)

    Kislov, Alexander

    2016-04-01

    Introduction. During the first quarter of the Holocene, the Black Sea (BS) experienced large changes: amid the gradually rising water surface, Black Sea level (BSL) fluctuations occurred. We calculated based on records (e.g., Balabanov, 2007) that the standard deviation is ˜3.5 ÷ 5 m. Their typical duration was ˜1000 years. Time of occurrence of positive and negative anomalies of the BS is different in different reconstructions. The source of these discrepancies could be tectonically induced vertical motions. Before ˜7 ka BP the BSL was higher than the level of the World Ocean. The rising BS spilled over a rocky sill at the Bosphorus (Chepalyga, 2007). It is clear that if the water discharge were quite large, the long-term BSL anomalies could not be. This study focuses on the quantification of this concept. Methodology. I use the equation of the water balance of the BS in term of the annual averaged level anomalies. Time scales of the BSL fluctuations were determined based on the BS basin morphology and averaged volumes of rivers runoff and water discharge via the Bosphorus Sill. The short-term (1-2 year) contribution (like random white noise) to level changes are due to variations of river runoff and precipitation mines evaporation. From this perspective, the water balance equation is represented as a stochastic Langevin equation (Kislov, 2015). In another case, the BSL anomaly could be destructed due to relation "BSL anomaly - value of water discharge via the Bosphorus Sill" which acts as a negative feedback. Results. To quantify the parameters, I use the present day information about hydrological regime of the BS. It should not lead to serious errors, because the first and last quarters of the Holocene exhibit similarity in their hydroclimatic regimes (Panin, Matlakhova, 2014). As well, the paleohydrological data about dynamics of the Dnieper River runoff was used (Swetc, 1978). It was found that the time scale of the BSL fluctuations due to water discharge

  13. Indonesian perceptions on the implementation of the chemical weapons convention in relation with biosecurity and biosafety

    International Nuclear Information System (INIS)

    Isroil, S.

    2009-01-01

    April 29, 2007 was marked the 10 year anniversary of the Chemical Weapons Convention (CWC) entry into force and the creation of the OPCW. Many nations throughout the last year were celebrated its commemoration. Compared to the Biological Weapons Convention (BWC) which is now entering the 33rd year of its entry into force, the progress of CWC is running far beyond that convention because CWC is considered the most complete convention which is equipped with a comprehensive verification system. In contrast, up till now there is no formal verification regime to monitor compliance of the BWC. So the national legislation as well as biosafety and biosecurity procedures will be the best regime to prohibit the misuse of biological agents. To some extent, the strategy and method on implementing the provision of CWC are coincident with biosecurity and biosafety procedure due to their dual use characteristics. Concerning CWC, Indonesia which was ratified it in 30 September 1998 has always active in any multilateral meeting and as well as national activities on prohibiting the misuse of chemical weapons. Several courses have also been done in cooperation with OPCW such as Development of Response System Against Chemical Weapons, Basic Training Course for Response Team, National Industry Awareness Workshop, Advance Training for Response Team, National Emergency Response Workshop, as well as setting up 20 sets of individual protective equipment. There have already 7 inspections done by OPCW in Indonesia during 2004-2007 which proved that there were no indications of misuse of chemical processes and its facilities for hostile purposes. However, it does not mean that there is no threat from the possible misuse of chemical and biological agents due to its dual use characteristics. Learnt from Indonesian experiences, there are several constraints on implementing the CWC as well as biosafety and biosecurity. First is the different perception on the biological and chemical threats. For

  14. CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns.

    Science.gov (United States)

    Taning, Clauvis Nji Tizi; Van Eynde, Benigna; Yu, Na; Ma, Sanyuan; Smagghe, Guy

    2017-04-01

    Discovered as a bacterial adaptive immune system, CRISPR/Cas9 (clustered, regularly interspaced, short palindromic repeat/CRISPR associated) is being developed as an attractive tool in genome editing. Due to its high specificity and applicability, CRISPR/Cas9-mediated gene editing has been employed in a multitude of organisms and cells, including insects, for not only fundamental research such as gene function studies, but also applied research such as modification of organisms of economic importance. Despite the rapid increase in the use of CRISPR in insect genome editing, results still differ from each study, principally due to existing differences in experimental parameters, such as the Cas9 and guide RNA form, the delivery method, the target gene and off-target effects. Here, we review current reports on the successes of CRISPR/Cas9 applications in diverse insects and insect cells. We furthermore summarize several best practices to give a useful checklist of CRISPR/Cas9 experimental setup in insects for beginners. Lastly, we discuss the biosafety concerns related to the release of CRISPR/Cas9-edited insects into the environment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Next generation inactivated polio vaccine manufacturing to support post polio-eradication biosafety goals.

    Directory of Open Access Journals (Sweden)

    Yvonne E Thomassen

    Full Text Available Worldwide efforts to eradicate polio caused a tipping point in polio vaccination strategies. A switch from the oral polio vaccine, which can cause circulating and virulent vaccine derived polioviruses, to inactivated polio vaccines (IPV is scheduled. Moreover, a manufacturing process, using attenuated virus strains instead of wild-type polioviruses, is demanded to enhance worldwide production of IPV, especially in low- and middle income countries. Therefore, development of an IPV from attenuated (Sabin poliovirus strains (sIPV was pursued. Starting from the current IPV production process based on wild type Salk strains, adaptations, such as lower virus cultivation temperature, were implemented. sIPV was produced at industrial scale followed by formulation of both plain and aluminium adjuvanted sIPV. The final products met the quality criteria, were immunogenic in rats, showed no toxicity in rabbits and could be released for testing in the clinic. Concluding, sIPV was developed to manufacturing scale. The technology can be transferred worldwide to support post polio-eradication biosafety goals.

  16. The biosafety of X-ray in bed; A biosseguranca dos raios-X no leito

    Energy Technology Data Exchange (ETDEWEB)

    Costa, A.S.; Vinco, Y.C.; Machado, C.P., E-mail: yasmin_claise@hotmail.com [Unigrancapital, Cidade Grande, MS (Brazil)

    2015-07-01

    This work aims to raise awareness on biosafety that the professional radiology needs to develop, for their own protection as well as the patient in bed during the examination of the X-ray. Assess why the use of many artifacts and discuss their safe use, the conditions necessary for operating activities that employ radioactive and radiological techniques are adopted for the benefit of society. Taking also into account the protection of workers, the public, and the patient environment. This study aims to evaluate the knowledge of technical professionals working in the field-performing x -rays in bed, currently academic course in radiology technologist. The results obtained show that 67 % of technicians, technologists’ future, use the personal protective equipment, and 25 % sometimes and never use 8 %, 92 %. Answered that in the period that is being performed on X -ray examination bed, but there are others bedridden in the same environment, with 88 % of patients in bed in bed not receive personal protective equipment nor collective protection equipment. Thus, we conclude that most technicians have cognition existing risks, so the individual protection measures are being carried out, but not by all. What still leaves to be desired is the protection of the patient in bed. These professionals need to be encouraged to study, so that we have trained professionals and holders of knowledge, enabling the improvement in labor and protection of professional and patient. (author)

  17. Laboratory diagnosis of Ebola virus disease and corresponding biosafety considerations in the China Ebola Treatment Center.

    Science.gov (United States)

    Huang, Qing; Fu, Wei-Ling; You, Jian-Ping; Mao, Qing

    2016-10-01

    Ebola virus disease (EVD), caused by Ebola virus (EBOV), is a potent acute infectious disease with a high case-fatality rate. Etiological and serological EBOV detection methods, including techniques that involve the detection of the viral genome, virus-specific antigens and anti-virus antibodies, are standard laboratory diagnostic tests that facilitate confirmation or exclusion of EBOV infection. In addition, routine blood tests, liver and kidney function tests, electrolytes and coagulation tests and other diagnostic examinations are important for the clinical diagnosis and treatment of EVD. Because of the viral load in body fluids and secretions from EVD patients, all body fluids are highly contagious. As a result, biosafety control measures during the collection, transport and testing of clinical specimens obtained from individuals scheduled to undergo EBOV infection testing (including suspected, probable and confirmed cases) are crucial. This report has been generated following extensive work experience in the China Ebola Treatment Center (ETC) in Liberia and incorporates important information pertaining to relevant diagnostic standards, clinical significance, operational procedures, safety controls and other issues related to laboratory testing of EVD. Relevant opinions and suggestions are presented in this report to provide contextual awareness associated with the development of standards and/or guidelines related to EVD laboratory testing.

  18. Nematode 18S rRNA gene is a reliable tool for environmental biosafety assessment of transgenic banana in confined field trials.

    Science.gov (United States)

    Nakacwa, R; Kiggundu, A; Talwana, H; Namaganda, J; Lilley, C; Tushemereirwe, W; Atkinson, H

    2013-10-01

    Information on relatedness in nematodes is commonly obtained by DNA sequencing of the ribosomal internal transcribed spacer region. However, the level of diversity at this locus is often insufficient for reliable species differentiation. Recent findings suggest that the sequences of a fragment of the small subunit nuclear ribosomal DNA (18S rRNA or SSU), identify genera of soil nematodes and can also distinguish between species in some cases. A database of soil nematode genera in a Ugandan soil was developed using 18S rRNA sequences of individual nematodes from a GM banana confined field trial site at the National Agricultural Research Laboratories, Kawanda in Uganda. The trial was planted to evaluate transgenic bananas for resistance to black Sigatoka disease. Search for relatedness of the sequences gained with entries in a public genomic database identified a range of 20 different genera and sometimes distinguished species. Molecular markers were designed from the sequence information to underpin nematode faunal analysis. This approach provides bio-indicators for disturbance of the soil environment and the condition of the soil food web. It is being developed to support environmental biosafety analysis by detecting any perturbance by transgenic banana or other GM crops on the soil environment.

  19. Biosafety Assessment of Microbial Strains Used in Biotechnology According to Their Taxonomy

    Directory of Open Access Journals (Sweden)

    Natalia I. Sheina

    2017-03-01

    Full Text Available A great variety of biotechnological products are now widely used in different ways in agriculture, medicine, food manufacturing and other areas of our life. Industrialized societies now more than ever depend on the use of genetically engineered products, with many of them synthesized using recombinant strains of microorganisms. There is an opinion that microbial strains used in biotechnology are potentially harmful for human health and the environment. Similar to many other countries, we have enacted environmental legislation in an effort to balance the risks and benefits of using biotechnological strains. Although environmental monitoring rules focus mainly on safety assessments of chemicals, the biosafety assessment of microbial strains used in biotechnology is a very important issue as well. This article summarizes 15 years of research on the biotechnological strains of microbes widely used as producers of various biological substances for industrial purposes, and their environmental and biotechnological applications. In our survey, we tried to evaluate possible adverse effects (general toxicity and damage to the immune system, potential sensitizing effects, and damage to normal microbiota caused by these microbes. It was shown that microscopical fungi of genera Aspergillus, Penicillium and Candida, and some gram-negative bacteria can affect the immune system and disrupt the normal balance of microbial flora of the intestinal tract in rats. The actinomycetes are less dangerous in that they cause fewer side effects. The investigation data obtained can be used to develop safety and hygienic standards for industrial microbes that will help decrease or minimize the occupational risk of infection or damage to the immune system when working with biotechnological strains of microbes.

  20. The ethics of biosafety considerations in gain-of-function research resulting in the creation of potential pandemic pathogens.

    Science.gov (United States)

    Evans, Nicholas Greig; Lipsitch, Marc; Levinson, Meira

    2015-11-01

    This paper proposes an ethical framework for evaluating biosafety risks of gain-of-function (GOF) experiments that create novel strains of influenza expected to be virulent and transmissible in humans, so-called potential pandemic pathogens (PPPs). Such research raises ethical concerns because of the risk that accidental release from a laboratory could lead to extensive or even global spread of a virulent pathogen. Biomedical research ethics has focused largely on human subjects research, while biosafety concerns about accidental infections, seen largely as a problem of occupational health, have been ignored. GOF/PPP research is an example of a small but important class of research where biosafety risks threaten public health, well beyond the small number of persons conducting the research.We argue that bioethical principles that ordinarily apply only to human subjects research should also apply to research that threatens public health, even if, as in GOF/PPP studies, the research involves no human subjects. Specifically we highlight the Nuremberg Code's requirements of 'fruitful results for the good of society, unprocurable by other methods', and proportionality of risk and humanitarian benefit, as broad ethical principles that recur in later documents on research ethics and should also apply to certain types of research not involving human subjects. We address several potential objections to this view, and conclude with recommendations for bringing these ethical considerations into policy development. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  1. The 10th International Symposium on the Biosafety of Genetically Modified Organisms (ISBGMO), Wellington, New Zealand, November 2008.

    Science.gov (United States)

    Sweet, Jeremy

    2009-01-01

    The Symposium consisted of eight sessions of oral presentations as well as various workshops and poster sessions. This report reviews the presentations in the following sessions and discusses the main conclusions and issues arising from each session: Session 1: Biosafety - experience and results Session 2: Introgression, invasion and fitness Session 3: Biotic and abiotic stress resistance Session 4: GM animals Session 5: Effects of GM crops on soil ecosystems Session 7: Biocontainment methods Session 8: Post market environmental monitoring Abstracts of the presentations in these sessions are available at: http://www.isbgmo.info/assets_/isbgmo_symposium_handbook.pdf.

  2. Princípios de biossegurança aplicados aos laboratórios de ensino universitário de microbiologia e parasitologia Principles of biosafety applied to microbiology and parasitology laboratories in universities

    Directory of Open Access Journals (Sweden)

    Luis Antônio Sangioni

    2013-01-01

    well as able to practice them properly to ensure the safety of all professionals, academics and the environment. This article compiles the main aspects related to the principles of biosafety, risk classification of biological agents and levels of biocontainment, and addresses the issues regarding safety equipment and good laboratory practices applied to the teaching laboratories in microbiology and parasitology.

  3. Biosafety and Biosecurity: A Relative Risk-Based Framework for Safer, More Secure, and Sustainable Laboratory Capacity Building.

    Science.gov (United States)

    Dickmann, Petra; Sheeley, Heather; Lightfoot, Nigel

    2015-01-01

    Laboratory capacity building is characterized by a paradox between endemicity and resources: countries with high endemicity of pathogenic agents often have low and intermittent resources (water, electricity) and capacities (laboratories, trained staff, adequate regulations). Meanwhile, countries with low endemicity of pathogenic agents often have high-containment facilities with costly infrastructure and maintenance governed by regulations. The common practice of exporting high biocontainment facilities and standards is not sustainable and concerns about biosafety and biosecurity require careful consideration. A group at Chatham House developed a draft conceptual framework for safer, more secure, and sustainable laboratory capacity building. The draft generic framework is guided by the phrase "LOCAL - PEOPLE - MAKE SENSE" that represents three major principles: capacity building according to local needs (local) with an emphasis on relationship and trust building (people) and continuous outcome and impact measurement (make sense). This draft generic framework can serve as a blueprint for international policy decision-making on improving biosafety and biosecurity in laboratory capacity building, but requires more testing and detailing development.

  4. Challenges and Practices in Building and Implementing Biosafety and Biosecurity Programs to Enable Basic and Translational Research with Select Agents.

    Science.gov (United States)

    Jonsson, Colleen B; Cole, Kelly Stefano; Roy, Chad J; Perlin, David S; Byrne, Gerald

    2013-04-29

    Select agent research in the United States must meet federally-mandated biological surety guidelines and rules which are comprised of two main components: biosecurity and biosafety. Biosecurity is the process employed for ensuring biological agents are properly safeguarded against theft, loss, diversion, unauthorized access or use/release. Biosafety is those processes that ensure that operations with such agents are conducted in a safe, secure and reliable manner. As such, a biological surety program is generally concerned with biological agents that present high risk for adverse medical and/or agricultural consequences upon release outside of proper containment. The U.S. Regional and National Biocontainment Laboratories (RBL, NBL) represent expertise in this type of research, and are actively engaged in the development of programs to address these critical needs and federal requirements. While this comprises an ongoing activity for the RBLs, NBLs and other facilities that handle select agents as new guidelines and regulations are implemented, the present article is written with the goal of presenting a simplified yet comprehensive review of these requirements. Herein, we discuss the requirements and the various activities that the RBL/NBL programs have implemented to achieve these metrics set forth by various agencies within the U.S. Federal government.

  5. Biosafety and Biosecurity: A relative risk-based framework for safer, more secure and sustainable laboratory capacity building

    Directory of Open Access Journals (Sweden)

    Petra eDickmann

    2015-10-01

    Full Text Available Background: Laboratory capacity building is characterized by a paradox between endemicity and resources: Countries with high endemicity of pathogenic agents often have low and intermittent resources (water, electricity and capacities (laboratories, trained staff, adequate regulations. Meanwhile, countries with low endemicity of pathogenic agents often have high containment facilities with costly infrastructure and maintenance governed by regulations. The common practice of exporting high biocontainment facilities and standards is not sustainable and concerns about biosafety and biosecurity require careful consideration. Methods: A group at Chatham House developed a draft conceptual framework for safer, more secure and sustainable laboratory capacity building. Results: The draft generic framework is guided by the phrase ‘LOCAL – PEOPLE – MAKE SENSE’ that represents three major principles: capacity building according to local needs (local with an emphasis on relationship and trust-building (people and continuous outcome and impact measurement (make sense. Conclusions: This draft generic framework can serve as a blueprint for international policy decision-making on improving biosafety and biosecurity in laboratory capacity building, but requires more testing and detailing development.

  6. Avian influenza surveillance with FTA cards: field methods, biosafety, and transportation issues solved.

    Science.gov (United States)

    Kraus, Robert H S; van Hooft, Pim; Waldenström, Jonas; Latorre-Margalef, Neus; Ydenberg, Ronald C; Prins, Herbert H T

    2011-08-02

    Avian Influenza Viruses (AIVs) infect many mammals, including humans(1). These AIVs are diverse in their natural hosts, harboring almost all possible viral subtypes(2). Human pandemics of flu originally stem from AIVs(3). Many fatal human cases during the H5N1 outbreaks in recent years were reported. Lately, a new AIV related strain swept through the human population, causing the 'swine flu epidemic'(4). Although human trading and transportation activity seems to be responsible for the spread of highly pathogenic strains(5), dispersal can also partly be attributed to wild birds(6, 7). However, the actual reservoir of all AIV strains is wild birds. In reaction to this and in face of severe commercial losses in the poultry industry, large surveillance programs have been implemented globally to collect information on the ecology of AIVs, and to install early warning systems to detect certain highly pathogenic strains(8-12). Traditional virological methods require viruses to be intact and cultivated before analysis. This necessitates strict cold chains with deep freezers and heavy biosafety procedures to be in place during transport. Long-term surveillance is therefore usually restricted to a few field stations close to well equipped laboratories. Remote areas cannot be sampled unless logistically cumbersome procedures are implemented. These problems have been recognised(13, 14) and the use of alternative storage and transport strategies investigated (alcohols or guanidine)(15-17). Recently, Kraus et al.(18) introduced a method to collect, store and transport AIV samples, based on a special filter paper. FTA cards(19) preserve RNA on a dry storage basis(20) and render pathogens inactive upon contact(21). This study showed that FTA cards can be used to detect AIV RNA in reverse-transcription PCR and that the resulting cDNA could be sequenced and virus genes and determined. In the study of Kraus et al.(18) a laboratory isolate of AIV was used, and samples were handled

  7. STEM VQ Method, Using Scanning Transmission Electron Microscopy (STEM) for Accurate Virus Quantification

    Science.gov (United States)

    2017-02-02

    WEEV in Ontario, Canada in 194126. This virus has a passage history including both animals and cell culture. Biosafety level (BSL-)-3 laboratory...Agarose-Based Plaque Assay Each virus stock was quantitated by standard agarose-based plaque assay23...samples used here were well prepared and the standard macro was used. As we have developed this method we have observed that while inferior

  8. Defense Laboratory Enterprise

    Science.gov (United States)

    2011-07-01

    NSWC - Corona Division Corona , CA 53 NSWC - Crane Division Crane, IN 55 NSWC - Dahlgren Division Dahlgren, VA 57 NSWC - Naval Explosive Ordnance...Invention • HemCon Chitosan Dressing – 2004 Army Greatest Invention • Combat Application Tourniquet ( CAT ) – 2005 Army Greatest Invention • Damage...laboratory within DoD with the capability to study highly hazardous viruses requiring maximum containment at Biosafety Level 4 (BSL-4). While the

  9. Análisis, implementación y evaluación de prácticas con el equipo Biopac BSL MP45 para el Laboratorio de Ingeniería Biomédica de la UPS

    OpenAIRE

    Montesdeoca Contreras, Julio César

    2014-01-01

    La Universidad Politécnica Salesiana lleva a cabo el proyecto "Diseño de un Laboratorio de Ingeniería Biomédica", el documento consta del diseño del manual para el equipo Biopac BSL MP45, prácticas de adquisición y reconocimiento de las bioseñales EMG, ECG, EEG y EOG; se describe la naturaleza de las bioseñales, la estructura de diseño de las prácticas así como de los protocolo por cada tipo de bioseñal a adquirir. The Universidad Politécnica Salesiana carried out the project "Design of a ...

  10. Fire Blight Control: The Struggle Goes On. A Comparison of Different Fire Blight Control Methods in Switzerland with Respect to Biosafety, Efficacy and Durability.

    Science.gov (United States)

    Gusberti, Michele; Klemm, Urs; Meier, Matthias S; Maurhofer, Monika; Hunger-Glaser, Isabel

    2015-09-11

    Fire blight (FB), caused by Erwinia amylovora, is one of the most important pome fruit pathogens worldwide. To control this devastating disease, various chemical and biological treatments are commonly applied in Switzerland, but they fail to keep the infection at an acceptable level in years of heavy disease pressure. The Swiss authorities therefore currently allow the controlled use of the antibiotic streptomycin against FB in years that are predicted to have heavy infection periods, but only one treatment per season is permitted. Another strategy for controlling Erwinia is to breed resistant/tolerant apple cultivars. One way of accelerating the breeding process is to obtain resistant cultivars by inserting one or several major resistance genes, using genetic engineering. To date, no study summarizing the impact of different FB control measures on the environment and on human health has been performed. This study consequently aims to compare different disease-control measures (biological control, chemical control, control by antibiotics and by resistant/tolerant apple cultivars obtained through conventional or molecular breeding) applied against E. amylovora, considering different protection goals (protection of human health, environment, agricultural diversity and economic interest), with special emphasis on biosafety aspects. Information on each FB control measure in relation to the specified protection goal was assessed by literature searches and by interviews with experts. Based on our results it can be concluded that the FB control measures currently applied in Switzerland are safe for consumers, workers and the environment. However, there are several gaps in our knowledge of the human health and environmental impacts analyzed: data are missing (1) on long term studies on the efficacy of most of the analyzed FB control measures; (2) on the safety of operators handling streptomycin; (3) on residue analyses of Equisetum plant extract, the copper and aluminum

  11. Safety (management and technology). Reality of biosafety technology; Anzen (manejimento to tekunoroji). Baio/sefuti gijutsu no jissai

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, H. [Chiyoda Technoace Co. Ltd., Yokohama (Japan)

    1994-08-05

    Biosafety, in other words, biohazard countermeasure is to prevent biological disasters, in particular, hazards caused by microbes infecting human beings or things, and they are roughly classified into two kinds. One is a disaster of infection via pathogenic microbes originally existing in the nature or allergens and toxins which microbes produce. The other is a potential, unknown hazards in the field of so-called biotechnology such as gene recombination technology. This paper describes the features of microbe pollution and measures against biohazard. The basis of the biohazard countermeasures is isolation from microbe pollution, and in reality confinement of microbes. As to methods for confining microbes, in addition to physical confinement, there is a biological confinement method using a host-vector system which is hard to exist in conditions other than special incubating conditions in recombination DNA experiments. 3 refs., 3 figs., 2 tabs.

  12. MALDI-TOF mass spectrometry and high-consequence bacteria: safety and stability of biothreat bacterial sample testing in clinical diagnostic laboratories.

    Science.gov (United States)

    Tracz, Dobryan M; Tober, Ashley D; Antonation, Kym S; Corbett, Cindi R

    2018-03-01

    We considered the application of MALDI-TOF mass spectrometry for BSL-3 bacterial diagnostics, with a focus on the biosafety of live-culture direct-colony testing and the stability of stored extracts. Biosafety level 2 (BSL-2) bacterial species were used as surrogates for BSL-3 high-consequence pathogens in all live-culture MALDI-TOF experiments. Viable BSL-2 bacteria were isolated from MALDI-TOF mass spectrometry target plates after 'direct-colony' and 'on-plate' extraction testing, suggesting that the matrix chemicals alone cannot be considered sufficient to inactivate bacterial culture and spores in all samples. Sampling of the instrument interior after direct-colony analysis did not recover viable organisms, suggesting that any potential risks to the laboratory technician are associated with preparation of the MALDI-TOF target plate before or after testing. Secondly, a long-term stability study (3 years) of stored MALDI-TOF extracts showed that match scores can decrease below the threshold for reliable species identification (<1.7), which has implications for proficiency test panel item storage and distribution.

  13. Investigation on Live Pig Insurance and Ill Dead Pig Biosafety Disposal in Xinzheng,Henan Province%河南新郑市的生猪保险联动

    Institute of Scientific and Technical Information of China (English)

    刘炜; 张代宝; 贾松涛; 赵立军; 周婷婷

    2014-01-01

    Dead pig biosafety disposal is related to public health,food safety and sustainable and health development of animal husbandry. In order to make clear the situation of pig farming,dead pig biosafety disposal,live pig insur-ance and quarantine supervision in Xinzheng City,Henan Province,investigations were carried out on biosafety dis-posal and live pig insurance. The achievements and problems were analyzed,and suggestions were proposed on dead pig biosafety disposal.%文章介绍了河南新郑市推行生猪保险与病死猪无害化处理联动的情况和成效。针对这一工作存在的问题提出了建立联动机制的建议。

  14. Detection of Ebola Virus RNA through Aerosol Sampling of Animal Biosafety Level 4 Rooms Housing Challenged Nonhuman Primates

    Science.gov (United States)

    2016-08-02

    Rooms Housing Challenged Nonhuman Primates 10 11 12 13 14 15 David E. Harbourt1*, Sara C. Johnston1, James Pettitt2, Travis K. Warren1 and...Sampling of ABSL-4 Rooms Housing Challenged Nonhuman 10 Primates for publication in an edition of The Journal of Infectious Disease. This 11 manuscript...embedded in the texts. This is the first report demonstrating detection of Ebola virus 17 RNA from animal rooms housing infected nonhuman primates and

  15. Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

    Directory of Open Access Journals (Sweden)

    Zhi-Dong Cao

    2016-01-01

    Full Text Available Background: Recently, local sustained-release antibiotics systems have been developed because they can increase local foci of concentrated antibiotics without increasing the plasma concentration, and thereby effectively decrease any systemic toxicity and side effects. A vancomycin-loaded bone-like hydroxyapatite/poly-amino acid (V-BHA/PAA bony scaffold was successfully fabricated with vancomycin-loaded poly lactic-co-glycolic acid microspheres and BHA/PAA, which was demonstrated to exhibit both porosity and perfect biodegradability. The aim of this study was to systematically evaluate the biosafety of this novel scaffold by conducting toxicity tests in vitro and in vivo. Methods: According to the ISO rules for medical implant biosafety, for in vitro tests, the scaffold was incubated with L929 fibroblasts or rabbit noncoagulant blood, with simultaneous creation of positive control and negative control groups. The growth condition of L929 cells and hemolytic ratio were respectively evaluated after various incubation periods. For in vivo tests, a chronic osteomyelitis model involving the right proximal tibia of New Zealand white rabbits was established. After bacterial identification, the drug-loaded scaffold, drug-unloaded BHA/PAA, and poly (methyl methacrylate were implanted, and a blank control group was also set up. Subsequently, the in vivo blood drug concentrations were measured, and the kidney and liver functions were evaluated. Results: In the in vitro tests, the cytotoxicity grades of V-BHA/PAA and BHA/PAA-based on the relative growth rate were all below 1. The hemolysis ratios of V-BHA/PAA and BHA/PAA were 2.27% and 1.42%, respectively, both below 5%. In the in vivo tests, the blood concentration of vancomycin after implantation of V-BHA/PAA was measured at far below its toxic concentration (60 mg/L, and the function and histomorphology of the liver and kidney were all normal. Conclusion: According to ISO standards, the V-BHA/PAA scaffold

  16. Bioseguridad en instalaciones médicas de atención primaria y secundaria Biosafety in the primary and secondary health care facilities

    Directory of Open Access Journals (Sweden)

    María del Carmen Pérez Cueto

    2007-03-01

    Full Text Available Debido a la emergencia y reemergencia de agentes infecciosos en los últimos años se procesan a diario en los laboratorios clínicos y de microbiología de salud gran número de muestras de material infeccioso que pueden ser peligrosas para el personal de los laboratorios, para otros trabajadores y para la comunidad. Es por ello que resulta importante capacitar al personal en materia de bioseguridad, para que las personas expuestas conozcan los riesgos a que están sometidas, los medios de protección a usar, y qué hacer en caso de accidente. En encuesta realizada a trabajadores de laboratorios se ha detectado escasa cultura en este tema, y los accidentes registrados con más frecuencia fueron: el derrame de material infeccioso, la rotura de tubos con cultivo y los pinchazos con agujas de jeringas. Resulta de interés realizar capacitación en cascada según el nivel de los trabajadores, e incluir a los directivos de las unidades, que son los máximos responsables de la seguridad biológica en estas.Due to the emergence and reemergence of infectious agents in the last years, a great number of samples of infectious material that may be dangerous for the lab personnel, for other workers, and for the community, are processed daily in the clinical and microbiological labs. That's why, it is important to train the personnel in the biosafety topic, so that those who are exposed know the risks, the protection means they should use, and what to do if an accident occurs. In a survey done among lab workers, it was detected little culture on this topic, and the most frequent accidents were: the spilling of infectious material, the breaking of test tubes with culture, and the pricks with syringe needles. A cascade training, according the level of the workers, including the managers that are the most responsible for the biological safety, is necessary.

  17. Fire Blight Control: The Struggle Goes On. A Comparison of Different Fire Blight Control Methods in Switzerland with Respect to Biosafety, Efficacy and Durability

    Directory of Open Access Journals (Sweden)

    Michele Gusberti

    2015-09-01

    Full Text Available Fire blight (FB, caused by Erwinia amylovora, is one of the most important pome fruit pathogens worldwide. To control this devastating disease, various chemical and biological treatments are commonly applied in Switzerland, but they fail to keep the infection at an acceptable level in years of heavy disease pressure. The Swiss authorities therefore currently allow the controlled use of the antibiotic streptomycin against FB in years that are predicted to have heavy infection periods, but only one treatment per season is permitted. Another strategy for controlling Erwinia is to breed resistant/tolerant apple cultivars. One way of accelerating the breeding process is to obtain resistant cultivars by inserting one or several major resistance genes, using genetic engineering. To date, no study summarizing the impact of different FB control measures on the environment and on human health has been performed. This study consequently aims to compare different disease-control measures (biological control, chemical control, control by antibiotics and by resistant/tolerant apple cultivars obtained through conventional or molecular breeding applied against E. amylovora, considering different protection goals (protection of human health, environment, agricultural diversity and economic interest, with special emphasis on biosafety aspects. Information on each FB control measure in relation to the specified protection goal was assessed by literature searches and by interviews with experts. Based on our results it can be concluded that the FB control measures currently applied in Switzerland are safe for consumers, workers and the environment. However, there are several gaps in our knowledge of the human health and environmental impacts analyzed: data are missing (1 on long term studies on the efficacy of most of the analyzed FB control measures; (2 on the safety of operators handling streptomycin; (3 on residue analyses of Equisetum plant extract, the copper

  18. A biossegurança ocupacional na perspectiva do estudante de enfermagem | Occupational biosafety from the perspective of nursing students

    Directory of Open Access Journals (Sweden)

    Rosane Teresinha Fontana

    2017-05-01

    Full Text Available Trata-se de uma pesquisa descritiva que teve como objetivo investigar as não conformidades quanto à biossegurança cometidas pelos profissionais de saúde durante o cuidado, sob a perspectiva do estudante de enfermagem. A coleta de dados foi feita por meio de um questionário autoaplicável junto a 80 estudantes, que foram analisados mediante análise temática. O estudo respeitou os preceitos éticos envolvidos com a pesquisa em seres humanos. A não conformidade prevalente refere-se à negligência quanto ao uso de Equipamentos de Proteção Individual pelos trabalhadores, justificada pela falta de tempo, sobrecarga e desconhecimento do trabalhador. Para melhorar este cenário, os acadêmicos sugeriram educação permanente em saúde. Pode-se inferir que a educação pode ser uma estratégia para a cultura de segurança do usuário e do trabalhador. =================================== This is a descriptive research that aimed to investigate the non-conformities regarding biosafety, committed by health professionals during service delivery, from the perspective of nursing students. Data collection was done through a self-administered questionnaire with 80 students-, and were analyzed through thematic analysis. The study respected the ethical precepts related to human research. The prevalent non-compliance refers to workers’ negligence to use Individual Protection Equipment by, justified by their lack of time, overload and lack of knowledge. Academics suggested permanent health education as a way of improving this scenario. It can be inferred that education can be a strategy for the safety culture of both the user and the worker.

  19. A Review of Laboratory-Acquired Infections in the Asia-Pacific: Understanding Risk and the Need for Improved Biosafety for Veterinary and Zoonotic Diseases

    Directory of Open Access Journals (Sweden)

    Jarunee Siengsanan-Lamont

    2018-03-01

    Full Text Available A rapid review was performed to determine (1 the number and causes of reported laboratory-acquired infections (LAI in the Asia-Pacific region; (2 their significance and threat to the community; (3 the primary risk factors associated with LAIs; (4 the consequences in the event of a LAI or pathogen escape; and (5 to make general recommendations regarding biosafety practices for diagnosis and research in the Asia-Pacific region. A search for LAI and zoonoses in the Asia-Pacific region using online search engines revealed a relatively low number of reports. Only 27 LAI reports were published between 1982 and 2016. The most common pathogens associated with LAIs were dengue virus, Arthroderma spp., Brucella spp., Mycobacterium spp., Rickettsia spp., and Shigella spp. Seventy-eight percent (21 out of 27 LAI reports occurred in high-income countries (i.e., Australia, Japan, South Korea, Singapore, and Taiwan where laboratories were likely to comply with international biosafety standards. Two upper-middle income countries (China (2, and Malaysia (2 and one lower-middle income country (India (2 reported LAI incidents. The majority of the reports (fifty-two percent (14/27 of LAIs occurred in research laboratories. Five LAI reports were from clinical or diagnostic laboratories that are considered at the frontier for zoonotic disease detection. Governments and laboratories in the Asia-Pacific region should be encouraged to report LAI cases as it provides a useful tool to monitor unintended release of zoonotic pathogens and to further improve laboratory biosafety. Non-reporting of LAI events could pose a risk of disease transmission from infected laboratory staff to communities and the environment. The international community has an important and continuing role to play in supporting laboratories in the Asia-Pacific region to ensure that they maintain the safe working environment for the staff and their families, and the wider community.

  20. Guidelines for safe work practices in human and animal medical diagnostic laboratories. Recommendations of a CDC-convened, Biosafety Blue Ribbon Panel.

    Science.gov (United States)

    Miller, J Michael; Astles, Rex; Baszler, Timothy; Chapin, Kimberle; Carey, Roberta; Garcia, Lynne; Gray, Larry; Larone, Davise; Pentella, Michael; Pollock, Anne; Shapiro, Daniel S; Weirich, Elizabeth; Wiedbrauk, Danny

    2012-01-06

    Prevention of injuries and occupational infections in U.S. laboratories has been a concern for many years. CDC and the National Institutes of Health addressed the topic in their publication Biosafety in Microbiological and Biomedical Laboratories, now in its 5th edition (BMBL-5). BMBL-5, however, was not designed to address the day-to-day operations of diagnostic laboratories in human and animal medicine. In 2008, CDC convened a Blue Ribbon Panel of laboratory representatives from a variety of agencies, laboratory organizations, and facilities to review laboratory biosafety in diagnostic laboratories. The members of this panel recommended that biosafety guidelines be developed to address the unique operational needs of the diagnostic laboratory community and that they be science based and made available broadly. These guidelines promote a culture of safety and include recommendations that supplement BMBL-5 by addressing the unique needs of the diagnostic laboratory. They are not requirements but recommendations that represent current science and sound judgment that can foster a safe working environment for all laboratorians. Throughout these guidelines, quality laboratory science is reinforced by a common-sense approach to biosafety in day-to-day activities. Because many of the same diagnostic techniques are used in human and animal diagnostic laboratories, the text is presented with this in mind. All functions of the human and animal diagnostic laboratory--microbiology, chemistry, hematology, and pathology with autopsy and necropsy guidance--are addressed. A specific section for veterinary diagnostic laboratories addresses the veterinary issues not shared by other human laboratory departments. Recommendations for all laboratories include use of Class IIA2 biological safety cabinets that are inspected annually; frequent hand washing; use of appropriate disinfectants, including 1:10 dilutions of household bleach; dependence on risk assessments for many activities

  1. Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors

    Directory of Open Access Journals (Sweden)

    Adam J. Hume

    2016-07-01

    Full Text Available Effective inactivation of biosafety level 4 (BSL-4 pathogens is vital in order to study these agents safely. Gamma irradiation is a commonly used method for the inactivation of BSL-4 viruses, which among other advantages, facilitates the study of inactivated yet morphologically intact virions. The reported values for susceptibility of viruses to inactivation by gamma irradiation are sometimes inconsistent, likely due to differences in experimental protocols. We analyzed the effects of common sample attributes on the inactivation of a recombinant vesicular stomatitis virus expressing the Zaire ebolavirus glycoprotein and green fluorescent protein. Using this surrogate virus, we found that sample volume and protein content of the sample modulated viral inactivation by gamma irradiation but that air volume within the sample container and the addition of external disinfectant surrounding the sample did not. These data identify several factors which alter viral susceptibility to inactivation and highlight the usefulness of lower biosafety level surrogate viruses for such studies. Our results underscore the need to validate inactivation protocols of BSL-4 pathogens using “worst-case scenario” procedures to ensure complete sample inactivation.

  2. Biosafety risk assessment approaches for insect-resistant genetically modified crops

    Directory of Open Access Journals (Sweden)

    Inaam Ullah

    2017-02-01

    Full Text Available Background: Environmental risk assessment (ERA is imperative for commercial release of insect resistant, genetically modified crops (IR-GMCs.An insect specific, spider venom peptideω-HXTX-Hv1a (Hvt was successfully expressed in cotton plants. The cotton plants producing Hvt protein have demonstrated resistance against economically important insect pest species. The study was performed to assess the effects of Hvt producing cotton plants on Honey bees (Apis mellifera. Methods: Three approaches were used to evaluate the effects of Hvt protein on adults of honeybees; whole plant assays in flight cages, in vitro assays with pollen of Hvt-cotton, and assays with elevated levels of purified Hvt protein. Pollens of Bt cotton or purified Bt proteins were used as control. Results: The field experiments did not yield any meaningful data due to high rate of mortality in all treatments including the control. However, the laboratory experiments provided conclusive results in which Hvt, purified or in pollens, did not affect the survival or longevity of the bees compared to the control. During the course of study we were able to compare the quality, effectiveness and economics of different experiments. Conclusions: We conclude that Hvt either purified or produced in cotton plants do not affect the survival or longevity of honey bees. We are also of the view that starting at laboratory level assays not only gives meaningful data but also saves a lot of time and money that can be spent on other important questions regarding safety of a particular transgenic crop. Hence, a purpose-based, tiered approach could be the best choice for pre-release ERA of IR-GMCs.

  3. Simultaneous reflection masking: dependency on direct sound level and hearing-impairment

    DEFF Research Database (Denmark)

    Buchholz, Jörg; Mihai, Paul Glad

    2008-01-01

    B-SL direct sound level, NH-listeners showed a binaural suppression effect for delays smaller than 7-10 ms and a binaural enhancement effect for larger delays. When decreasing the direct sound level to 15 dB-SL, the only significant change observed was that the dichotic RMT increased for delays larger than...... expected from changed auditory filter bandwidth and audi-bility. However, the stimulus level-dependency of the auditory filters’ bandwidth was not reflected in the SRMT data....

  4. Biosafety Procedure for Safe Handling of Genetically Modified Plant Materials in Bio Design Facility

    International Nuclear Information System (INIS)

    Zaiton Ahmad; Shuhaimi Shamsudin; Mohamed Najli Mohamed Yasin; Affrida Abu Hassan; Mohd Zaid Hassan; Rusli Ibrahim

    2015-01-01

    Bio Design Facility is the specifically designed glass house for propagation, screening and analysis of high quality plant varieties developed through biotechnology or a combination of nuclear technology and biotechnology. High quality plant varieties especially genetically modified plants (GMO) require a special glass house facility for propagation and screening to isolate them from cross-pollinating with wild type varieties in surrounding ecosystem, and for carrying out evaluation of possible risks of the plants to human, animal and environment before they are proven safe for field trials or commercial release. This facility which was developed under the Ninth Malaysia Plan is classified as the Plant Containment Level 2 and is compliance with the bio safety regulations and guidance for the safe release of GMO according to Malaysian Bio safety Act 2007. Bio Design Facility is fully operational since 2010 and in 2012, it has also been certified as the glass house for post-entry quarantine by The Department of Agriculture. This paper summarizes the bio safety procedure for a safe, controlled and contained growing and evaluation of GMO in Bio Design Facility. This procedure covers the physical (containment and equipment's) and operational (including responsibility, code of practice, growing, decontamination and disposal of plant materials, emergency and contingency plan) aspects of the facility. (author)

  5. Communication and computing technology in biocontainment laboratories using the NEIDL as a model.

    Science.gov (United States)

    McCall, John; Hardcastle, Kath

    2014-07-01

    The National Emerging Infectious Diseases Laboratories (NEIDL), Boston University, is a globally unique biocontainment research facility housing biosafety level 2 (BSL-2), BSL-3, and BSL-4 laboratories. Located in the BioSquare area at the University's Medical Campus, it is part of a national network of secure facilities constructed to study infectious diseases of major public health concern. The NEIDL allows for basic, translational, and clinical phases of research to be carried out in a single facility with the overall goal of accelerating understanding, treatment, and prevention of infectious diseases. The NEIDL will also act as a center of excellence providing training and education in all aspects of biocontainment research. Within every detail of NEIDL operations is a primary emphasis on safety and security. The ultramodern NEIDL has required a new approach to communications technology solutions in order to ensure safety and security and meet the needs of investigators working in this complex building. This article discusses the implementation of secure wireless networks and private cloud computing to promote operational efficiency, biosecurity, and biosafety with additional energy-saving advantages. The utilization of a dedicated data center, virtualized servers, virtualized desktop integration, multichannel secure wireless networks, and a NEIDL-dedicated Voice over Internet Protocol (VoIP) network are all discussed. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  6. A estruturação do Programa de Capacitação Profissional de Biossegurança no contexto do Projeto de Modernização da Gestão Científica do Instituto Oswaldo Cruz Development of a Biosafety Training Program aligned with the Scientific Management Modernization Project of the Oswaldo Cruz Institute

    Directory of Open Access Journals (Sweden)

    Maria Eveline de Castro Pereira

    2010-06-01

    Scientific Management Modernization Project of IOC. The program, including the "Biosafety Course in Biomedical Research Laboratory", was structured according to the planning-development-evaluation cycle. Initially, for the diagnosis cycle, the IOC laboratories representatives answered a questionnaire that showed that both professional categories (middle and higher levels were interested in participating in BTP and mentioned, as preferential themes, biosafety and good laboratory practices. In the planning phase it was defined that BTP would be divided into two projects (Good Laboratory Practices in Public Health for middle level professionals and Biosafety Course in Biomedical Research Laboratories for higher level professionals. During the development phase of the Biosafety Course, the following modules were chosen: introduction, chemical, physical and biological risks, quality management and animal experimentation. Thus, in the period from 2006 to 2008, 315 professionals were trained and the respective evaluations were performed according to David Kirkpatrick's model. The first level of evaluation, called reaction, showed that 54.03% of the professionals said that the course was excellent, 39.59% classified the course as good and 6.38% as regular or did not express any opinion. For learning evaluation, pre and post-tests were carried out in each module. All the modules showed an increase in the grades of the post-test when compared to the pre-test. The results pointed to strategies that should be followed in order to improve this biosafety continuing education model.

  7. Biosafety of Prions.

    Science.gov (United States)

    Bistaffa, Edoardo; Rossi, Martina; De Luca, Chiara M G; Moda, Fabio

    2017-01-01

    Prions are the infectious agents that cause devastating and untreatable disorders known as Transmissible Spongiform Encephalopathies (TSEs). The pathologic events and the infectious nature of these transmissible agents are not completely understood yet. Due to the difficulties in inactivating prions, working with them requires specific recommendations and precautions. Moreover, with the advent of innovative technologies, such as the Protein Misfolding Cyclic Amplification (PMCA) and the Real Time Quaking-Induced Conversion (RT-QuIC), prions could be amplified in vitro and the infectious features of the amplified products need to be carefully assessed. © 2017 Elsevier Inc. All rights reserved.

  8. Medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad Biosafety measures adopted in Liorad Laboratories for handling biological materials

    Directory of Open Access Journals (Sweden)

    Nancy Burguet Lago

    2013-03-01

    Full Text Available Introducción: el trabajo con microorganismos puede conllevar a riesgos tanto para el personal que trabaja con los mismos como para el medio ambiente. La existencia de laboratorios de seguridad biológica y la implementación de medidas en la manipulación de los agentes biológicos minimizan el riesgo. Objetivo: evaluar las medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad. Métodos: empleo de una lista de chequeo y análisis de los resultados a través de una Matriz DAFO para valorar si el diseño de la instalación cumple con la bioseguridad. Además establecer un sistema documental para la manipulación de microorganismos y la confección de un plan de capacitación para el personal que trabaja en el laboratorio de control microbiológico. Resultados: la lista de chequeo permitió identificar como principal debilidad el no disponer de un doble pasillo para el traslado del material limpio y sucio. Como fortalezas, cumplir con las prácticas y procesamientos adecuados y el contar con equipos de seguridad biológica. El sistema documental incorporó a los procedimientos establecidos para la manipulación, un acápite referido a la «Peligrosidad y Medidas de Seguridad». El programa de capacitación desarrollado permitió proveer conocimientos específicos referidos a esta temática. Conclusión: las medidas adoptadas en el laboratorio permiten plantear que de manera general se cumplen los requisitos establecidos en materia de Bioseguridad para el trabajo con microorganismos.Introduction: working with microorganisms can lead to risks for both the staff at work and the environment. The existence of biosafety labs and implementation of measures in the handling of biological agents minimize the risk. Objetive: to evaluate biosecurity measures taken in handling biological materials at Liorad Laboratories. Methods: using a checklist and analysis of results through a SWOT Matrix to assess whether the

  9. In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety.

    Science.gov (United States)

    de Almeida, Isabela Neves; Aleixo, Agdemir Valéria; Carvalho, Wânia da Silva; de Miranda, Silvana Spindola

    2015-01-01

    The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples. Hence, the purpose of this study was to utilize the insertion sequence IS6110 for amplification of DNA from a smear-positive sample for tuberculosis (TB) diagnosis. Among the 52 positive bacilloscopies, sensitivity, specificity, positive predictive value and negative predictive value were 52.3%, 100%, 100% and 89.7%, respectively whereas accuracy was 90.7%. The IS6110-based PCR for TB diagnosis developed in DNA extracted from a positive smear is a fast, simple, specific, and safe method. Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  10. Evaluation of Carpet Steam and Heat Cleaners as Biological Sampling Devices

    Science.gov (United States)

    2016-05-01

    ABSTRACT c. THIS PAGE 19b. TELEPHONE NUMBER (include area code) U U U UU 34 (410) 436-7545 Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18...performed in the BD laboratories. The BD biosafety level (BSL) 1 and 2 facilities are fully equipped to handle a broad range of work that requires the...average density and standard deviation from five CR coupons was calculated: Area per coupon = 10 cm2 (or 1.55 in.2) Area per

  11. Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs

    Directory of Open Access Journals (Sweden)

    McCurdy Kimberly S

    2010-01-01

    Full Text Available Abstract Background Embryonated chicken eggs (ECE are sometimes used for the primary isolation or passage of influenza viruses, other viruses, and certain bacteria. For small-scale experiments with pathogens that must be studied in biosafety level three (BSL3 facilities, inoculated ECE are sometimes manipulated and maintained in small egg incubators within a biosafety cabinet (BSC. To simplify the clean up and decontamination of an egg incubator in case of egg breakage, we explored whether ethylene breather bags could be used to encase ECE inoculated with pathogens. This concept was tested by determining embryo survival and examining virus yields in bagged ECE. Results Virus yields acceptable for many applications were attained when influenza-, alpha-, flavi-, canine distemper-, and mousepox viruses were propagated in ECE sealed within ethylene breather bags. Conclusions For many small-scale applications, ethylene breather bags can be used to encase ECE inoculated with various viruses.

  12. Hospital seguro frente aos desastres: uma reflexão sobre biossegurança e arquitetura Hospitals safe from disasters: a reflection on architecture and biosafety

    Directory of Open Access Journals (Sweden)

    Lucia Cristina de Paiva Saba

    2012-02-01

    Full Text Available Um dos maiores desafios da sociedade atual é o enfrentamento das adversidades causadas pelos desastres. Os estabelecimentos de saúde, principalmente os hospitais, são considerados essenciais nessas situações. Este trabalho discute os princípios da arquitetura do hospital seguro frente aos desastres, como propõem a Organização Mundial da Saúde e a Organização Pan-Americana da Saúde. Projetar um hospital seguro exige ações multidisciplinares, com envolvimento de administradores, arquitetos, engenheiros, médicos e enfermeiros. O planejamento de cada hospital pressupõe uma análise de riscos e aspectos de segurança específicos. Também é importante agregar a biossegurança ao conceito de hospital seguro. O equilíbrio entre aspectos arquitetônicos e biossegurança permite a compreensão dos riscos associados ao trabalho, facilitando o dimensionamento de espaços para suportar as ações de resposta frente às emergências. Em suma, a programação de um hospital seguro requer uma síntese de conhecimentos que relacionam diversos saberes, entre eles os da biossegurança e da arquitetura hospitalar. Esses princípios devem embasar as indagações sobre o hospital seguro e o planejamento de projetos arquitetônicos com foco na manutenção das instalações em capacidade máxima mesmo diante de situações adversas.One of the biggest challenges in today’s society is facing adversity caused by disasters. Health facilities, especially hospitals, are considered essential in these situations. This article discusses the principles of architectural design of hospitals safe from disasters, as proposed by the World Health Organization and the Pan American Health Organization. Designing a safe hospital requires multidisciplinary efforts, involving administrators, architects, engineers, physicians, and nurses. The planning of each hospital demands the analysis of specific risks and safety concerns. The concept of biosafety should also be

  13. A importância da abordagem contextual no ensino de biossegurança The importance of the contextual approach in the teaching of biosafety

    Directory of Open Access Journals (Sweden)

    Maria Eveline de Castro Pereira

    2012-06-01

    Full Text Available A biossegurança é um campo do conhecimento que insere questões voltadas para organismos geneticamente modificados e relacionadas à proteção social e ocupacional do trabalhador. O seu processo educativo visa à formação de um agente participativo-transformador e, portanto, deve ultrapassar a simples ideia da normalização. Dessa forma, é importante contextualizar a biossegurança dentro de uma estratégia de ensino construtivista com a identificação dos seus conceitos estruturantes - risco, perigo e acidente - que permitam ao indivíduo compreender como o risco é percebido na sociedade e enfocado na academia para, em seguida, agregar múltiplas competências e enfrentá-lo. Conhecer como as relações de trabalho-saúde, suas implicações e impactos vem sendo construídas ao longo do tempo, pode formar um cidadão mais crítico e preparado para participar das decisões de ordem político-social que podem influenciar o seu futuro.Biosafety is a field of knowledge that raises questions geared to genetically modified organisms that are linked to social and job-related employee protection. The educational process involves seeking to create a participative and transforming agent and must therefore transcend the simple concept of teaching. Thus, it is important to contextualize biosafety within a constructive teaching strategy by identification of its core concepts - risk, hazard and accident - which allows each individual to understand how risk is perceived within society and dealt with in academia in order to add multiple skills to tackle the situation. Understanding how the relationship between work and health and its consequences and effects are constructed over the course of time, makes it possible to train more critical and well prepared citizens to participate in decisions of a political and social nature that can influence their future.

  14. Harmonized biosafety regulations are key to trust building in regional agbiotech partnerships: the case of the Bt cotton project in East Africa

    Directory of Open Access Journals (Sweden)

    Ezezika Obidimma C

    2012-11-01

    Full Text Available Abstract Background The Bacillus thuringiensis (Bt cotton public-private partnership (PPP project in East Africa was designed to gather baseline data on the effect of Bt cotton on biodiversity and the possibility of gene flow to wild cotton varieties. The results of the project are intended to be useful for Kenya, Uganda, and Tanzania when applying for biosafety approvals. Using the backdrop of the different biosafety regulations in the three countries, we investigate the role of trust in the Bt cotton partnership in East Africa. Methods Data were collected by reviewing relevant project documents and peer-reviewed articles on Bt cotton in Tanzania, Kenya and Uganda; conducting face-to-face interviews with key informants of the project; and conducting direct observations of the project. Data were analyzed based on recurring and emergent themes to create a comprehensive narrative on how trust is understood and built among the partners and with the community. Results We identified three factors that posed challenges to building trust in the Bt cotton project in East Africa: different regulatory regimes among the three countries; structural and management differences among the three partner institutions; and poor public awareness of GM crops and negative perceptions of the private sector. The structural and management differences were said to be addressed through joint planning, harmonization of research protocols, and management practices, while poor public awareness of GM crops and negative perceptions of the private sector were said to be addressed through open communication, sharing of resources, direct stakeholder engagement and awareness creation. The regulatory differences remained outside the scope of the project. Conclusions To improve the effectiveness of agbiotech PPPs, there is first a need for a regulatory regime that is acceptable to both the public and private sector partners. Second, early and continuous joint planning; sharing of

  15. A biosafety evaluation of synchrotron radiation X-ray to skin and bone marrow: single dose irradiation study of rats and macaques.

    Science.gov (United States)

    Lu, Yifan; Tang, Guanghui; Lin, Hui; Lin, Xiaojie; Jiang, Lu; Yang, Guo-Yuan; Wang, Yongting

    2017-06-01

    Very limited experimental data is available regarding the safe dosages related to synchrotron radiation (SR) procedures. We used young rats and macaques to address bone marrow and skin tolerance to various doses of synchrotron radiation. Rats were subjected to 0, 0.5, 2.5, 5, 25 or 100 Gy local SR X-ray irradiation at left hind limb. Rat blood samples were analyzed at 2-90 days after irradiation. The SR X-ray irradiated skin and tibia were sectioned for morphological examination. For non-human primate study, three male macaques were subjected to 0.5 or 2.5 Gy SR X-ray on crus. Skin responses of macaques were observed. All rats that received SR X-ray irradiation doses greater than 2.5 Gy experienced hair loss and bone-growth inhibition, which were accompanied by decreased number of follicles, thickened epidermal layer, and decreased density of bone marrow cells (p X-ray but showed significant hair loss when the dose was raised above 2.5 Gy. The safety threshold doses of SR X-ray for rat skin, bone marrow and macaque skin are between 0.5 and 2.5 Gy. Our study provided essential information regarding the biosafety of SR X-ray irradiation.

  16. Enzymatic activity of fungi isolated from crops

    Directory of Open Access Journals (Sweden)

    Wioletta A. Żukiewicz-Sobczak

    2016-12-01

    Full Text Available Aim: To detect and assess the activity of extracellular hydrolytic enzymes and to find differences in enzymograms between fungi isolated from wheat and rye samples and grown on Czapek-Dox Broth and Sabouraud Dextrose Broth enriched with cereal (wheat or rye. Isolated strains were also classified in the scale of biosafety levels (BSL. Material and methods: The study used 23 strains of fungi cultured from samples of wheat and rye (grain, grain dust obtained during threshing and soil collected in the Lublin region (eastern Poland. API ZYM test (bioMérieux was carried out according to the manufacturer’s instructions. Classification of BSL (Biosafety levels was based on the current literature. Results : High enzymatic activity was found in strains cultured in media containing 1% of wheat grain ( Bipolaris holmi, Penicillium decumbens and with an addition of 1% of rye grain ( Cladosporium herbarum, Aspergillus versicolor, Alternaria alternata . The total number of enzymes varied depending on the type of media, and in most cases it was higher in the culture where an addition of cereal grains was used. Conclusions : Isolated strains of fungi reveal differences in the profiles of the enzyme assay. It can be assumed that the substrate enriched in grains stimulate the higher activity of mold enzymes. Key words: enzymatic activity, mold fungi, zymogram, biohazards.

  17. Biossegurança e a dimensão subjetiva do trabalho e do risco Biosafety and the subjective dimension of work and risk

    Directory of Open Access Journals (Sweden)

    Marli B. M. de Albuquerque Navarro

    2009-01-01

    Full Text Available O artigo discute as possibilidades do campo reflexivo que se abre para a biossegurança, utilizando como ponto de partida interpretações de análises que abordam o mundo do trabalho, considerando as relações entre labor e subjetividade. Enfoca a dinâmica do trabalho, valorizando a questão da subjetividade como elemento de possibilidade criativa e inovadora no mundo do trabalho, analisando a articulação entre o pensamento com várias habilidades, incluindo a precisão dos gestos, o engajamento do corpo, a mobilização da inteligência, a capacidade de refletir, de interpretar e de reagir às situações, apresentando a plenitude do sentir associado ao pensar, como estímulo ao processo de criar, de inventar, contexto onde podemos identificar plenamente a dinâmica da atividade laboratorial e sua relação com a construção e a superação dos contextos de risco.This paper discusses the possibilities of the reflective field that opens for biosafety, using as a starting point interpretations of analyses that address the world of work, considering the relationship between labor and subjectivity. It focuses on the work dynamics, highlighting the issue of subjectivity as creative and innovative opportunity in the world of work, examining the relation among thought with several skills, including the accuracy of gestures, the engagement of the body, the mobilization of intelligence, the ability to reflect, to interpret and to react to situations, with full feeling associated with thinking, stimulating the process of creation, invention, where we can fully identify the dynamics of laboratory activity and its relation with the construction and overcoming of risk contexts.

  18. Deglacial sea level history of the East Siberian Sea and Chukchi Sea margins

    Science.gov (United States)

    Cronin, Thomas M.; O'Regan, Matt; Pearce, Christof; Gemery, Laura; Toomey, Michael; Semiletov, Igor; Jakobsson, Martin

    2017-09-01

    Deglacial (12.8-10.7 ka) sea level history on the East Siberian continental shelf and upper continental slope was reconstructed using new geophysical records and sediment cores taken during Leg 2 of the 2014 SWERUS-C3 expedition. The focus of this study is two cores from Herald Canyon, piston core SWERUS-L2-4-PC1 (4-PC1) and multicore SWERUS-L2-4-MC1 (4-MC1), and a gravity core from an East Siberian Sea transect, SWERUS-L2-20-GC1 (20-GC1). Cores 4-PC1 and 20-GC were taken at 120 and 115 m of modern water depth, respectively, only a few meters above the global last glacial maximum (LGM; ˜ 24 kiloannum or ka) minimum sea level of ˜ 125-130 meters below sea level (m b.s.l.). Using calibrated radiocarbon ages mainly on molluscs for chronology and the ecology of benthic foraminifera and ostracode species to estimate paleodepths, the data reveal a dominance of river-proximal species during the early part of the Younger Dryas event (YD, Greenland Stadial GS-1) followed by a rise in river-intermediate species in the late Younger Dryas or the early Holocene (Preboreal) period. A rapid relative sea level rise beginning at roughly 11.4 to 10.8 ka ( ˜ 400 cm of core depth) is indicated by a sharp faunal change and unconformity or condensed zone of sedimentation. Regional sea level at this time was about 108 m b.s.l. at the 4-PC1 site and 102 m b.s.l. at 20-GC1. Regional sea level near the end of the YD was up to 42-47 m lower than predicted by geophysical models corrected for glacio-isostatic adjustment. This discrepancy could be explained by delayed isostatic adjustment caused by a greater volume and/or geographical extent of glacial-age land ice and/or ice shelves in the western Arctic Ocean and adjacent Siberian land areas.

  19. A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

    Directory of Open Access Journals (Sweden)

    Holbrook Michael R

    2009-07-01

    Full Text Available Abstract Nipah virus (NiV and Hendra virus (HeV are the only paramyxoviruses requiring Biosafety Level 4 (BSL-4 containment. Thus, study of henipavirus entry at less than BSL-4 conditions necessitates the use of cell-cell fusion or pseudotyped reporter virus assays. Yet, these surrogate assays may not fully emulate the biological properties unique to the virus being studied. Thus, we developed a henipaviral entry assay based on a β-lactamase-Nipah Matrix (βla-M fusion protein. We first codon-optimized the bacterial βla and the NiV-M genes to ensure efficient expression in mammalian cells. The βla-M construct was able to bud and form virus-like particles (VLPs that morphologically resembled paramyxoviruses. βla-M efficiently incorporated both NiV and HeV fusion and attachment glycoproteins. Entry of these VLPs was detected by cytosolic delivery of βla-M, resulting in enzymatic and fluorescent conversion of the pre-loaded CCF2-AM substrate. Soluble henipavirus receptors (ephrinB2 or antibodies against the F and/or G proteins blocked VLP entry. Additionally, a Y105W mutation engineered into the catalytic site of βla increased the sensitivity of our βla-M based infection assays by 2-fold. In toto, these methods will provide a more biologically relevant assay for studying henipavirus entry at less than BSL-4 conditions.

  20. The impact of regulations, safety considerations and physical limitations on research progress at maximum biocontainment.

    Science.gov (United States)

    Shurtleff, Amy C; Garza, Nicole; Lackemeyer, Matthew; Carrion, Ricardo; Griffiths, Anthony; Patterson, Jean; Edwin, Samuel S; Bavari, Sina

    2012-12-01

    We describe herein, limitations on research at biosafety level 4 (BSL-4) containment laboratories, with regard to biosecurity regulations, safety considerations, research space limitations, and physical constraints in executing experimental procedures. These limitations can severely impact the number of collaborations and size of research projects investigating microbial pathogens of biodefense concern. Acquisition, use, storage, and transfer of biological select agents and toxins (BSAT) are highly regulated due to their potential to pose a severe threat to public health and safety. All federal, state, city, and local regulations must be followed to obtain and maintain registration for the institution to conduct research involving BSAT. These include initial screening and continuous monitoring of personnel, controlled access to containment laboratories, accurate and current BSAT inventory records. Safety considerations are paramount in BSL-4 containment laboratories while considering the types of research tools, workflow and time required for conducting both in vivo and in vitro experiments in limited space. Required use of a positive-pressure encapsulating suit imposes tremendous physical limitations on the researcher. Successful mitigation of these constraints requires additional time, effort, good communication, and creative solutions. Test and evaluation of novel vaccines and therapeutics conducted under good laboratory practice (GLP) conditions for FDA approval are prioritized and frequently share the same physical space with important ongoing basic research studies. The possibilities and limitations of biomedical research involving microbial pathogens of biodefense concern in BSL-4 containment laboratories are explored in this review.

  1. The Impact of Regulations, Safety Considerations and Physical Limitations on Research Progress at Maximum Biocontainment

    Directory of Open Access Journals (Sweden)

    Jean Patterson

    2012-12-01

    Full Text Available We describe herein, limitations on research at biosafety level 4 (BSL-4 containment laboratories, with regard to biosecurity regulations, safety considerations, research space limitations, and physical constraints in executing experimental procedures. These limitations can severely impact the number of collaborations and size of research projects investigating microbial pathogens of biodefense concern. Acquisition, use, storage, and transfer of biological select agents and toxins (BSAT are highly regulated due to their potential to pose a severe threat to public health and safety. All federal, state, city, and local regulations must be followed to obtain and maintain registration for the institution to conduct research involving BSAT. These include initial screening and continuous monitoring of personnel, controlled access to containment laboratories, accurate and current BSAT inventory records. Safety considerations are paramount in BSL-4 containment laboratories while considering the types of research tools, workflow and time required for conducting both in vivo and in vitro experiments in limited space. Required use of a positive-pressure encapsulating suit imposes tremendous physical limitations on the researcher. Successful mitigation of these constraints requires additional time, effort, good communication, and creative solutions. Test and evaluation of novel vaccines and therapeutics conducted under good laboratory practice (GLP conditions for FDA approval are prioritized and frequently share the same physical space with important ongoing basic research studies. The possibilities and limitations of biomedical research involving microbial pathogens of biodefense concern in BSL-4 containment laboratories are explored in this review.

  2. The Impact of Regulations, Safety Considerations and Physical Limitations on Research Progress at Maximum Biocontainment

    Science.gov (United States)

    Shurtleff, Amy C.; Garza, Nicole; Lackemeyer, Matthew; Carrion, Ricardo; Griffiths, Anthony; Patterson, Jean; Edwin, Samuel S.; Bavari, Sina

    2012-01-01

    We describe herein, limitations on research at biosafety level 4 (BSL-4) containment laboratories, with regard to biosecurity regulations, safety considerations, research space limitations, and physical constraints in executing experimental procedures. These limitations can severely impact the number of collaborations and size of research projects investigating microbial pathogens of biodefense concern. Acquisition, use, storage, and transfer of biological select agents and toxins (BSAT) are highly regulated due to their potential to pose a severe threat to public health and safety. All federal, state, city, and local regulations must be followed to obtain and maintain registration for the institution to conduct research involving BSAT. These include initial screening and continuous monitoring of personnel, controlled access to containment laboratories, accurate and current BSAT inventory records. Safety considerations are paramount in BSL-4 containment laboratories while considering the types of research tools, workflow and time required for conducting both in vivo and in vitro experiments in limited space. Required use of a positive-pressure encapsulating suit imposes tremendous physical limitations on the researcher. Successful mitigation of these constraints requires additional time, effort, good communication, and creative solutions. Test and evaluation of novel vaccines and therapeutics conducted under good laboratory practice (GLP) conditions for FDA approval are prioritized and frequently share the same physical space with important ongoing basic research studies. The possibilities and limitations of biomedical research involving microbial pathogens of biodefense concern in BSL-4 containment laboratories are explored in this review. PMID:23342380

  3. In silico Allergenicity Study of Insect resistant genetically Modified Rice (Oryza sativa L. for assessment of biosafety

    Directory of Open Access Journals (Sweden)

    S.K. Das

    2017-12-01

    Full Text Available India is one of the world's largest producers of rice (Oryza sativa, accounting for 20% of all world rice production. However, lepidopteran pests severely impact the harvest of rice, which leads to environmental pollution and increase production cost. Alternatively, genetic engineering methods may be used to prevent rice pests and increase production of rice in a safe and environmentally friendly manner. Bacillus thuringiensis (Bt genes have been widely used to generate genetically modified (GM crops because the expressed cry1Ab protein confers resistance to lepidopteron pests. The proteins expressed by these genes may lead to food safety problems. Thus, safety evaluations are necessary prior to commercialization. Bioinformatics analysis for allergenicity assessment of cry1Ab protein is performed using different allergen databases viz. FARRP SDAP, Allergome, and Algpred to identify any potential sequence matches to allergen proteins that might indicate allergenic cross-reactivity with the query sequence. A full FASTA search was performed to identify highly similar proteins. However; the full length search cannot identify discontinuous or conformational epitopes that depend upon the tertiary structure of the protein.So every possible contiguous 80-amino acid sequence of each query protein was searched for determining the similarity. The proteins sequence can be searched using FASTA/BLAST for broad homology to known allergens to identify any short sequence that might represent an allergenic epitope. The domains in the Cry protein sequences were searched using Interproscan for potential similarity at the domain level. The results showed neither significant alignment nor similarity of cry1Ab protein at full sequence, domain, and epitope level with any of the known allergen proteins in the full sequence matching. Matching the 80 amino acid and matching of 8 amino acids showed no similarity to determine the epitope potential. From literature survey

  4. Regulatory and biosafety issues in relation to transgenic animals in food and agriculture, feeds containing genetically modified organisms (GMO) and veterinary biologics

    International Nuclear Information System (INIS)

    Kochhar, H.P.S.; Gifford, G.A.; Kahn, S.

    2005-01-01

    Development of an effective regulatory system for genetically engineered animals and their products has been the subject of increasing discussion among researchers, industry and policy developers, as well as the public. Since transgenesis and cloning are relatively new scientific techniques, transgenic animals are new organisms for which there is limited information. The issues associated with the regulation and biosafety of transgenic animals pertain to environmental impact, human food safety, animal health and welfare, trade and ethics. To regulate this new and powerful technology predicated on limited background information is a challenge not only for the regulators, but also for the developers of such animals, who strive to prove that the animals are safe and merit bio-equivalency to their conventional counterparts. In principle, an effective regulatory sieve should permit safe products while forming a formidable barrier for those assessed of posing an unacceptable risk. Adoption of transgenic technology for use in agriculture will depend upon various factors that range from perceived benefits for humans and animals, to safe propagation, animal welfare considerations and integrity of species, as well as effects on bio-diversity. A regulatory framework designed to address the concerns connected with the environmental release of transgenic animals needs to also take into account the ability of genetically modified animals to survive and compete with conventional populations. Regulatory initiatives for biotechnology-derived animals and their products should ensure high standards for human and animal health; a sound scientific basis for evaluation; transparency and public involvement; and maintenance of genetic diversity. Feeds obtained by use of biotechnology have to be evaluated for animal and human safety by using parameters that define their molecular characterization, nutritional qualities and toxicological aspects, while veterinary biologics derived from

  5. Sociolinguistic Variation and Change in British Sign Language Number Signs: Evidence of Leveling?

    Science.gov (United States)

    Stamp, Rose; Schembri, Adam; Fenlon, Jordan; Rentelis, Ramas

    2015-01-01

    This article presents findings from the first major study to investigate lexical variation and change in British Sign Language (BSL) number signs. As part of the BSL Corpus Project, number sign variants were elicited from 249 deaf signers from eight sites throughout the UK. Age, school location, and language background were found to be significant…

  6. Biosafety in manned space flight

    International Nuclear Information System (INIS)

    De Boever, P.

    2006-01-01

    The main goal of manned exploration is to achieve a prolonged stay in space, for example in an orbital station (such as the International Space Station (ISS)) or in planetary bases on the Moon and/or Mars. It goes without saying that such missions can only be realized when the astronaut's health and well-being is secured. In this respect, the characterization of the microbiological contamination on board spacecraft and orbital stations and the influence of cosmic radiation and microgravity are of paramount importance. Microbial contamination may originate from different sources and includes the initial contamination of space flight materials during manufacturing and assembly, the delivery of supplies to the orbital station, the supplies themselves, secondary contamination during the lifetime of the orbital station, the crew and any other biological material on board e.g. animals, plants, micro-organisms used in scientific experiments. Although most microorganisms do not threaten human health, it has been reported that in a confined environment, such as a space cabin, microorganisms may produce adverse effects on the optimal performance of the space crew and the integrity of the spacecraft or habitat. These effects range from infections, allergies, and toxicities to degradation of air and water supplies. Biodegradation of critical materials may result in system failure and this may jeopardize the crew. The research aims at monitoring the biological airborne and surface contamination during manned space flight. The ISS has been selected as primary test bed for this study. The majority of the investigations are being done by the Russian Institute of Biomedical Problems (IBMP), which is responsible for monitoring the biological contamination in the habitable compartments of the ISS for safety and hygienic reasons. Within the frame of a collaboration between IBMP and the European Space Agency (ESA), SCK-CEN is able to participate in the analyses

  7. Os limites do poder das empresas multinacionais: o caso do Protocolo de Cartagena The limits of power of multinational companies: the Cartagena protocol on biosafety

    Directory of Open Access Journals (Sweden)

    Gilberto Sarfati

    2008-06-01

    internationalization tend - nationally and transnationally - to oppose the political actions of the MNCs. The second part of the work shows the role of MNCs in the negotiations of the Cartagena Protocol of Biosafety, a complementary instrument of the Biodiversity Convention (CBD, which regulates the genetically modified organisms (GMOs. An important conclusion of this work is to explain under which circumstances the MNCs are able to act as negotiators using their influence as well as their structural and soft powers, by trying to form transnational coalitions and by helping to build coalitions among national governments in order to defend their interests in intergovernmental negotiation processes.

  8. Educação em biossegurança e bioética: articulação necessária em biotecnologia Education on biosafety and bioethics: necessary articulation in biotechnology

    Directory of Open Access Journals (Sweden)

    Marcos De Bonis

    2009-12-01

    Full Text Available A educação científica tem sido debatida em alguns segmentos da sociedade e organizações internacionais têm estimulado as nações a investirem nessa área tão estratégica. Nesse contexto, a educação em biossegurança e bioética explora um conteúdo rico em prevenção, normas e princípios éticos, que servem para nortear os caminhos trilhados pela biotecnologia. A valorização da biossegurança e da bioética como parte de uma política educacional científica, efetiva e consistente, pode estimular a formação de indivíduos com uma consciência científica e cidadã, em condições de participar das questões de natureza ética e tecnológica produzidas pela biotecnologia.Science education has been discussed in some segments of the society and, international organizations have encouraged nations to invest in this strategic area. In this context, education in bioethics and biosafety explores a rich content on prevention, standards and ethical principles which serve to guide the paths track by biotechnology. The recovery of bioethics and biosafety, as part of an educational policy scientific, effective and consistent, can stimulate the formation of individuals with a scientific and citizen awareness, in a position to participate on ethical and technological issues produced by biotechnology.

  9. Bounding Accident Analysis for LLNL BSL-3 Facility

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, Mark [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2010-09-14

    The conclusion of this evaluation is that the consequence estimates in the EA can be reproduced using a public-accessible Gaussian plume-dispersion model and conservative modeling assumptions consistent with the accident scenario postulated in the EA. Also, the potential consequences to the public for the postulated accident would be far below the minimum infectious dose of one organism.

  10. 《卡塔赫纳生物安全议定书》与WTO规则冲突研究%Study on Conflicts between the Cartagena Protocol on Biosafety and WTO Rules

    Institute of Scientific and Technical Information of China (English)

    陈海君

    2005-01-01

    In the late thirty years biotechnology, with transgenic technology as its core,has been developed rapidly and applied in fields such as agriculture and medicine with great achievements, which has caused profound impacts on society and economy, However because of scientific uncertainties of influences of transgenrc technology and derived products on human, plant and animal life and health as well as the environment,biosafety。i.e management of biotechnology so as to minimize its potential risks to the environment and human health, has become an issue with increasing concern of the internatronal community,In this context, negotiation of an international treaty was initiated in 1996 and the Cartagena Protocol on Biosafety was adopted in January 2000 and came into effect in September 2003 ,As the first international treaty containing direct stipulations on living modified organisms (LMOs) trade, the Protocol encountered divergence and controversy in the negotiation process, After adoption of the Protocol, compatibility of its provisions with those set in the WTO agreements has turned into a hot issue for dispute in the international forum。

  11. Validation of Autoclave Protocols for Successful Decontamination of Category A Medical Waste Generated from Care of Patients with Serious Communicable Diseases.

    Science.gov (United States)

    Garibaldi, Brian T; Reimers, Mallory; Ernst, Neysa; Bova, Gregory; Nowakowski, Elaine; Bukowski, James; Ellis, Brandon C; Smith, Chris; Sauer, Lauren; Dionne, Kim; Carroll, Karen C; Maragakis, Lisa L; Parrish, Nicole M

    2017-02-01

    In response to the Ebola outbreak in 2014, many hospitals designated specific areas to care for patients with Ebola and other highly infectious diseases. The safe handling of category A infectious substances is a unique challenge in this environment. One solution is on-site waste treatment with a steam sterilizer or autoclave. The Johns Hopkins Hospital (JHH) installed two pass-through autoclaves in its biocontainment unit (BCU). The JHH BCU and The Johns Hopkins biosafety level 3 (BSL-3) clinical microbiology laboratory designed and validated waste-handling protocols with simulated patient trash to ensure adequate sterilization. The results of the validation process revealed that autoclave factory default settings are potentially ineffective for certain types of medical waste and highlighted the critical role of waste packaging in successful sterilization. The lessons learned from the JHH validation process can inform the design of waste management protocols to ensure effective treatment of highly infectious medical waste. Copyright © 2017 American Society for Microbiology.

  12. The Impact on Non-proliferation with a Growing Number of BL-3 and BL-4 Labs in South America

    International Nuclear Information System (INIS)

    Espona, M. J.

    2007-01-01

    After 2001 events (9/11 and anthrax letters) we witnessed the dramatic growth of the biodefense sector, and in within this framework, the increment of the number of high biosafety level laboratories (BSL3 and BSL4) both in central countries and peripheral ones. It is important to point out that not all the labs are devoted to activities related to biodefense but also to biological research from a traditional perspective. In this scenario, and as an unwanted consequence, we have noticed that the number of people -professionals with different level specialization- which have the information and skills that could be used to produce BW, grew vertiginously as well. So, considering the biodefense sector from a systemic perspective, we are being witnesses of how due to an intent to protect ourselves against the biological threat, our vulnerability to it gains an unexpected magnitude. Therefore, in this paper we will analyze the phenomenon of the growing number of high security level labs, first, in a global perspective and later, with focus in South America in order to determine if this evolving situation represents a security problem. In order to do that, we will approach the problem from different perspectives: health care, development, security/safety, defense and non proliferation.(author)

  13. A small animal peripheral challenge model of yellow fever using interferon-receptor deficient mice and the 17D-204 vaccine strain.

    Science.gov (United States)

    Thibodeaux, Brett A; Garbino, Nina C; Liss, Nathan M; Piper, Joseph; Blair, Carol D; Roehrig, John T

    2012-05-02

    Yellow fever virus (YFV), a member of the genus Flavivirus, is a mosquito-borne pathogen that requires wild-type (wt), virulent strains to be handled at biosafety level (BSL) 3, with HEPA-filtration of room air exhaust (BSL3+). YFV is found in tropical regions of Africa and South America and causes severe hepatic disease and death in humans. Despite the availability of effective vaccines (17D-204 or 17DD), YFV is still responsible for an estimated 200,000 cases of illness and 30,000 deaths annually. Besides vaccination, there are no other prophylactic or therapeutic strategies approved for use in human YF. Current small animal models of YF require either intra-cranial inoculation of YF vaccine to establish infection, or use of wt strains (e.g., Asibi) in order to achieve pathology. We have developed and characterized a BSL2, adult mouse peripheral challenge model for YFV infection in mice lacking receptors for interferons α, β, and γ (strain AG129). Intraperitoneal challenge of AG129 mice with 17D-204 is a uniformly lethal in a dose-dependent manner, and 17D-204-infected AG129 mice exhibit high viral titers in both brain and liver suggesting this infection is both neurotropic and viscerotropic. Furthermore the use of a mouse model permitted the construction of a 59-biomarker multi-analyte profile (MAP) using samples of brain, liver, and serum taken at multiple time points over the course of infection. This MAP serves as a baseline for evaluating novel therapeutics and their effect on disease progression. Changes (4-fold or greater) in serum and tissue levels of pro- and anti-inflammatory mediators as well as other factors associated with tissue damage were noted in AG129 mice infected with 17D-204 as compared to mock-infected control animals. Published by Elsevier Ltd.

  14. U.S. Ebola Treatment Center Clinical Laboratory Support.

    Science.gov (United States)

    Jelden, Katelyn C; Iwen, Peter C; Herstein, Jocelyn J; Biddinger, Paul D; Kraft, Colleen S; Saiman, Lisa; Smith, Philip W; Hewlett, Angela L; Gibbs, Shawn G; Lowe, John J

    2016-04-01

    Fifty-five hospitals in the United States have been designated Ebola treatment centers (ETCs) by their state and local health authorities. Designated ETCs must have appropriate plans to manage a patient with confirmed Ebola virus disease (EVD) for the full duration of illness and must have these plans assessed through a CDC site visit conducted by an interdisciplinary team of subject matter experts. This study determined the clinical laboratory capabilities of these ETCs. ETCs were electronically surveyed on clinical laboratory characteristics. Survey responses were returned from 47 ETCs (85%). Forty-one (87%) of the ETCs planned to provide some laboratory support (e.g., point-of-care [POC] testing) within the room of the isolated patient. Forty-four (94%) ETCs indicated that their hospital would also provide clinical laboratory support for patient care. Twenty-two (50%) of these ETC clinical laboratories had biosafety level 3 (BSL-3) containment. Of all respondents, 34 (72%) were supported by their jurisdictional public health laboratory (PHL), all of which had available BSL-3 laboratories. Overall, 40 of 44 (91%) ETCs reported BSL-3 laboratory support via their clinical laboratory and/or PHL. This survey provided a snapshot of the laboratory support for designated U.S. ETCs. ETCs have approached high-level isolation critical care with laboratory support in close proximity to the patient room and by distributing laboratory support among laboratory resources. Experts might review safety considerations for these laboratory testing/diagnostic activities that are novel in the context of biocontainment care. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  15. A Rapid Screen for Host-Encoded miRNAs with Inhibitory Effects against Ebola Virus Using a Transcription- and Replication-Competent Virus-Like Particle System

    Directory of Open Access Journals (Sweden)

    Zhongyi Wang

    2018-05-01

    Full Text Available MicroRNAs (miRNAs may become efficient antiviral agents against the Ebola virus (EBOV targeting viral genomic RNAs or transcripts. We previously conducted a genome-wide search for differentially expressed miRNAs during viral replication and transcription. In this study, we established a rapid screen for miRNAs with inhibitory effects against EBOV using a tetracistronic transcription- and replication-competent virus-like particle (trVLP system. This system uses a minigenome comprising an EBOV leader region, luciferase reporter, VP40, GP, VP24, EBOV trailer region, and three noncoding regions from the EBOV genome and can be used to model the life cycle of EBOV under biosafety level (BSL 2 conditions. Informatic analysis was performed to select up-regulated miRNAs targeting the coding regions of the minigenome with the highest binding energy to perform inhibitory effect screening. Among these miRNAs, miR-150-3p had the most significant inhibitory effect. Reverse transcription polymerase chain reaction (RT-PCR, Western blot, and double fluorescence reporter experiments demonstrated that miR-150-3p inhibited the reproduction of trVLPs via the regulation of GP and VP40 expression by directly targeting the coding regions of GP and VP40. This novel, rapid, and convenient screening method will efficiently facilitate the exploration of miRNAs against EBOV under BSL-2 conditions.

  16. Involvement of Pro-Inflammatory Macrophages in Liver Pathology of Pirital Virus-Infected Syrian Hamsters

    Directory of Open Access Journals (Sweden)

    Corey L. Campbell

    2018-05-01

    Full Text Available New World arenaviruses cause fatal hemorrhagic disease in South America. Pirital virus (PIRV, a mammarenavirus hosted by Alston’s cotton rat (Sigmodon alstoni, causes a disease in Syrian golden hamsters (Mesocricetus auratus (biosafety level-3, BSL-3 that has many pathologic similarities to the South American hemorrhagic fevers (BSL-4 and, thus, is considered among the best small-animal models for human arenavirus disease. Here, we extend in greater detail previously described clinical and pathological findings in Syrian hamsters and provide evidence for a pro-inflammatory macrophage response during PIRV infection. The liver was the principal target organ of the disease, and signs of Kupffer cell involvement were identified in mortally infected hamster histopathology data. Differential expression analysis of liver mRNA revealed signatures of the pro-inflammatory response, hematologic dysregulation, interferon pathway and other host response pathways, including 17 key transcripts that were also reported in two non-human primate (NHP arenavirus liver-infection models, representing both Old and New World mammarenavirus infections. Although antigen presentation may differ among rodent and NHP species, key hemostatic and innate immune-response components showed expression parallels. Signatures of pro-inflammatory macrophage involvement in PIRV-infected livers included enrichment of Ifng, Nfkb2, Stat1, Irf1, Klf6, Il1b, Cxcl10, and Cxcl11 transcripts. Together, these data indicate that pro-inflammatory macrophage M1 responses likely contribute to the pathogenesis of acute PIRV infection.

  17. Grain dust originating from organic and conventional farming as a potential source of biological agents causing respiratory diseases in farmers.

    Science.gov (United States)

    Zukiewicz-Sobczak, Wioletta A; Cholewa, Grażyna; Krasowska, Ewelina; Chmielewska-Badora, Jolanta; Zwoliński, Jacek; Sobczak, Paweł

    2013-12-01

    Agricultural producers are exposed to a number of different health risks associated with their work environment. The objective of the study was to assess the degree of colonization by fungi in terms of quantity and in terms of variety of species the samples taken from the settled dust from combine threshing of rye cultivation from organic and conventional farms in the Province of Lublin. This paper is a preliminary quantitative assessment of the species of fungi colonizing the samples of settled dust collected during combine threshing from organic and conventional farms in the Province of Lublin. One of the stages of the project was the classification of biosafety BSL (biosafety level) of selected isolates and API ZYM tests to evaluate the potential ability of isolates to cause adverse health effects. To determine the concentration and composition of fungi in collected samples plate dilution method was used with two media: Malt Agar and Potato Dextrose Agar. MOST COMMONLY ISOLATED FUNGI IN SETTLED DUST SAMPLES COLLECTED DURING COMBINE THRESHING FROM ORGANIC FARMS, ON PDA MEDIUM WERE: Alternaria alternata and Aureobasidium pullulans. Cultures on MA medium were dominated by Alternaria alternata, Mycelia sterilia and Fusarium poae. In samples of dust from conventional crops, the predominant species was Alternaria alternata on PDA medium and on MA medium. The obtained results show a potential risk of people involved in agricultural work.

  18. The stability and degradation of dietary DNA in the gastrointestinal tract of mammals: implications for horizontal gene transfer and the biosafety of GMOs.

    Science.gov (United States)

    Rizzi, Aurora; Raddadi, Noura; Sorlini, Claudia; Nordgrd, Lise; Nielsen, Kaare Magne; Daffonchio, Daniele

    2012-01-01

    The fate of dietary DNA in the gastrointestinal tract (GIT) of animals has gained renewed interest after the commercial introduction of genetically modified organisms (GMO). Among the concerns regarding GM food, are the possible consequences of horizontal gene transfer (HGT) of recombinant dietary DNA to bacteria or animal cells. The exposure of the GIT to dietary DNA is related to the extent of food processing, food composition, and to the level of intake. Animal feeding studies have demonstrated that a minor amount of fragmented dietary DNA may resist the digestive process. Mammals have been shown to take up dietary DNA from the GIT, but stable integration and expression of internalized DNA has not been demonstrated. Despite the ability of several bacterial species to acquire external DNA by natural transformation, in vivo transfer of dietary DNA to bacteria in the intestine has not been detected in the few experimental studies conducted so far. However, major methodological limitations and knowledge gaps of the mechanistic aspects of HGT calls for methodological improvements and further studies to understand the fate of various types of dietary DNA in the GIT.

  19. Ensino de Biossegurança na Graduação em Enfermagem: uma revisão da literatura Enseñanza de Bioseguridad en la Graduación de Enfermería: una revisión de la literatura The teaching of Biosafety in Nursing Undergraduation: a literature review

    Directory of Open Access Journals (Sweden)

    Andréia de Carvalho Andrade

    2007-10-01

    Full Text Available Trata-se de um estudo que realizou um levantamento da produção científica em enfermagem referente ao ensino de biossegurança com o objetivo de investigar o contexto em que se deram e conhecer o conteúdo das publicações acerca do ensino de biossegurança na graduação em enfermagem. Foram analisados artigos de periódicos encontrados na consulta às bases de dados LILACS, SciELO, MEDLINE, BDENF, DEDALUS e PERIENF, identificando 26 artigos. Os resultados encontrados proporcionaram a formação e discussão das categorias: "Bases ideológicas e teóricas", "Abordagem histórica de biossegurança", "Riscos ocupacionais e AIDS" e "Educação em biossegurança na formação". Conclui-se que o número de artigos é pequeno, principalmente artigos enfocando educação em biossegurança, mas houve uma evolução deste conteúdo nos últimos anos.Se trata de un estudio que realizá un levantamiento de la produccion científica en enfermería referente o la enseñanza de bioseguridad con el objetivo de investigar el contexto en que se dieron y conocer el contenido de las publicacions acerca de la ensenonze de bioseguridad em la graduación de enfermería. Fueron analizados artículos de periódicos encontrados em la consulta de bases de datos LILACS, SciELO, MEDLINE, BDENF, DEDALUS y PERIENF, identificando 26 artículos. Los resultados encontrados proporcionaron la formacíon y discusión de las categorías: "Bases ideológicas y teóricas", "Abordaje histórica de bioseguridad", "Riesgos ocupacionales y SIDA" y "Educación en bioseguridad en la formación". Se concluye que el número de artículos es pequeno, principalmente artículos que enfocan educación en bioseguridad, pero hubo una evolución de este contenido en los últimos años.It's a study that made a scientific production survey in nursing regarding to biosafety teaching aiming to investigate its context and discover the content of publishing on biosafety teaching in the nursing

  20. Assessing biosafety of GM plants containing lectins

    DEFF Research Database (Denmark)

    Poulsen, Morten; Pedersen, Jan W.

    2010-01-01

    insects. However, since the cry genes are not active against all insects, e.g. sap-sucking insects, other genes coding for proteins such as lectins show promise of complementing the cry genes for insect resistance. As with other novel plants, lectin-expressing plants will need to be assessed...... for their potential risks to human and animal health and the environment. The expressed lectin protein should be assessed on its own for potential toxicity and allergenicity as for any other new protein. Although not many lectins have been thoroughly tested for their toxicity, our evaluation suggests that most...... of the lectins that are potentially useful for insect resistance will pose no health risk in genetically modified (GM) plants. Since some lectins are known for their toxicity to humans, the insertion of lectin genes in food crop plants will have to be assessed carefully. It is expected that in some cases...

  1. The bioethics and biosafety of gene transfer

    DEFF Research Database (Denmark)

    Madsen, Kathrine H.; Sandøe, Peter

    2008-01-01

    things, indirect and long-term environmental effects are now included. Other major socio-economic concerns — e.g. lack of demonstrated usefulness to society, and the consumer’s right to choose non-GM food products — have been debated. This debate has led to regulations designed to permit the co...

  2. agri-biotech applications' biosafety initiative

    African Journals Online (AJOL)

    these workshops are: Mexico, Costa Rica, in Mexico being the recipient, Monsanto (USA). Argentina, Uruguay, Paraguay, Brazil, technology donor, and Rockefeller Foundation. Malaysia, Thailand, the People's Republic of financial donor; (4) training of a Mexican. China, Indonesia and the Philippines. scientist in the USA in ...

  3. Conhecimento e adesão às recomendações de biossegurança no Corpo de Bombeiros Militar de Minas Gerais Conocimiento y adhesión a las recomendaciones de bioseguridad en el cuerpo militar de bomberos de Minas Gerais Knowledge and adherence to biosafety recommendations in a military fire brigade in Minas Gerais

    Directory of Open Access Journals (Sweden)

    Adriana Cristina Oliveira

    2013-02-01

    bioseguridad, aunque existe la necesidad de enfatizar la importancia de realizar un esquema de vacunación contra la hepatitis B.This study aimed to assess the knowledge and attitudes concerning adherence to biosafety recommendations of the military personnel of Belo Horizonte's Military Fire Brigade in Minas Gerais . A cross-sectional study was conducted from January to February 2011. A structured questionnaire was used, consisting of questions regarding knowledge and attitudes related to biosafety and the factors hindering the use of personal protective equipment. The results showed that the personnel had adequate knowledge of hand hygiene (90.4% and standard precautions (89%, such as the habit of always wearing gloves (95.7%, but lacked knowledge about the antiseptic activity of 70% alcohol (77.5%. The cloak was mentioned as the piece of personal protective equipment that was most difficult to use. However, most of the personnel were unaware of their serological status for hepatitis B (25%. Their knowledge and attitudes were consistent with the biosafety recommendations, but the importance of hepatitis B immunization must be emphasized.

  4. Biossegurança: conhecimento e adesão pelos profissionais do corpo de bombeiros militar de Minas Gerais Bioseguridad: conocimiento y adhesión en el cuerpo de bomberos militar de Minas Gerais, uno estudio transversal Biosafety: knowledge and compliance by fire military brigade of Minas Gerais

    Directory of Open Access Journals (Sweden)

    Adriana Cristina de Oliveira

    2013-03-01

    como intención provocar la reflexión de los profesionales y de las instituciones responsables, cuanto a la revisión de la propuesta relacionada con la formación de estos trabajadores en bioseguridad y salud ocupacional.It was aimed to identify knowledge and adherence of biosafety recommendations by military Firefighters of Minas Gerais from a city in the metropolitan area of Belo Horizonte, Minas Gerais. A cross-sectional study of quantitative approach was held. The study instrument was a structured questionnaire that included questions about knowledge and attitudes related to biosafety, complicating factors for adherence to standard precautions and incidence of accidents involving biological materials. It was demonstrated knowledge about hand hygiene recommendation in 84,8%, 69,7% didn't know the way of alcohol 70% action. The scrubs were cited as the most difficult personal protective equipment to be used. The militaries haven't had any accident with biological material, similar data to other from literature. It is intended to provoke reflection of the professionals in the area and institutions responsible about the reassessment of the proposal related to the formation of these workers in biosafety and occupational health.

  5. Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus

    Directory of Open Access Journals (Sweden)

    Aysen eGargili

    2013-08-01

    Full Text Available Crimean-Congo hemorrhagic fever virus (CCHFV is one of the most geographically widespread arboviruses and causes a severe hemorrhagic syndrome in humans. The virus circulates in nature in a vertebrate-tick cycle and ticks of the genus Hyalomma are the main vectors and reservoirs. Although the tick vector plays a central role in the maintenance and transmission of CCHFV in nature, comparatively little is known of CCHFV-tick interactions. This is mostly due to the fact that establishing tick colonies is laborious, and working with CCHFV requires a biosafety level 4 laboratory (BSL4 in many countries. Nonetheless, an in vivo transmission model is essential to understand the epidemiology of the transmission cycle of CCHFV. In addition, important parameters such as vectorial capacity of tick species, levels of infection in the host necessary to infect the tick, and aspects of virus transmission by tick bite including the influence of tick saliva, cannot be investigated any other way. Here, we evaluate the influence of different laboratory animal species as hosts supporting the life cycle of Hyalomma marginatum, a two-host tick. Rabbits were considered the host of choice for the maintenance of the uninfected colonies due to high larval attachment rates, shorter larval-nymphal feeding times, higher nymphal molting rates, high egg hatching rates and higher conversion efficiency index. Furthermore, we describe the successful establishment of an in vivo transmission model CCHFV in a BSL4 biocontainment setting using interferon knockout mice. This will give us a new tool to study the transmission and interaction of CCHFV with its tick vector.

  6. Lathe leveler

    Energy Technology Data Exchange (ETDEWEB)

    Lovelady, III, Michael W.J.

    2018-03-06

    A lathe leveler for centering a cutting tool in relation to a cylindrical work piece includes a first leveling arm having a first contact point disposed adjacent a distal end of the first leveling arm, a second leveling arm having a second contact point disposed adjacent a distal end of the second leveling arm, a leveling gage, and a leveling plate having a cutting tool receiving surface positioned parallel to a horizontal axis of the leveling gage and on a same plane as a midpoint of the first contact point and the second contact point. The leveling arms and leveling plate are dimensioned and configured such that the cutting tool receiving surface is centered in relation to the work piece when the first and second contact points are in contact with one of the inner surface and outer surface of the cylindrical work piece and the leveling gage is centered.

  7. Changes in Plasma Sex Hormone Levels in Women with Severe Concomitant Injury

    Directory of Open Access Journals (Sweden)

    K. N Yezhova

    2010-01-01

    Full Text Available Objective: to perform a complex study of the plasma levels of 11 sex hormones and their functional values in women with severe concomitant injury (SCI. Subjects and methods. The study enrolled 16 women aged 18—45 years who had SCI. Admission APACHE II scores were 18.9±1.3. According to the outcome of a posttraumatic period, all the patients were divided into 2 groups: A survivors; B deceased subjects. The normal values were used to comparatively analyze the concentrations of reproductive hormones. The time course of changes in hormone concentration was studied on postoperative days 1, 3, and 7. The hormone profile was examined by BSL test kits (USA on a STAT Fax 2100 enzyme immunoanalyzer (Awareness Technology Inc., USA. The content of prolactin, luteinizing hormone, follicle-stimulating hormone, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate (DHEA-S, androstendione (A, testosterone (T, dihydrotestosterone, estrone, and estradiol (E were measured. Results. The complex study of changes in the profile of 11 plasma sex hormones was first conducted in women in the posttraumat-ic period. Moreover, the typical plasma hormonal changes were elevated prolactin levels, a decrease in the concentrations of gonadotropins, and increases in some androgens, A, T, and E. The deceased women showed lower concentrations of DHEA-S and T. Analysis revealed an inverse correlation between the plasma concentration of DHEA-S and the injury severity. This change seems to suggest that an adrenal adaptation reaction is exhausted. The changes revealed in hormonal levels are of significance in understanding the pathogenesis of SCT. This may serve as a basis for the development of new therapy modalities using reproductive hormones in the postresuscitative period. Key words: severe concomitant injury, sex hormones, prolactin, luteinizing hormone, follicle-stimulating hormone, progesterone, 17-hydroxyprogesterone, androgens, estrogens.

  8. Cortisol level

    Science.gov (United States)

    ... enable JavaScript. The cortisol blood test measures the level of cortisol in the blood. Cortisol is a ... in the morning. This is important, because cortisol level varies throughout the day. You may be asked ...

  9. Triglyceride level

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003493.htm Triglyceride level To use the sharing features on this page, please enable JavaScript. The triglyceride level is a blood test to measure the amount ...

  10. Level 1 - level 2 interface

    International Nuclear Information System (INIS)

    Boneham, P.

    2003-01-01

    The Plant Damage States (PDS) are the starting point for the level 2 analysis. A PDS is group of core damage sequences that are expected to have similar severe accident progressions. In this paper an overview of Level 1/Level 2 interface, example PDS parameters, example PDS definitions using codes and example Bridge Tree are presented. PDS frequency calculation (identification of sequences for each PDS in level 1,split some CD sequences which have different level 2 progressions), code calculations providing support for grouping decisions and timings as well as PDS frequencies and definitions input to level 2 are also discussed

  11. Level densities

    International Nuclear Information System (INIS)

    Ignatyuk, A.V.

    1998-01-01

    For any applications of the statistical theory of nuclear reactions it is very important to obtain the parameters of the level density description from the reliable experimental data. The cumulative numbers of low-lying levels and the average spacings between neutron resonances are usually used as such data. The level density parameters fitted to such data are compiled in the RIPL Starter File for the tree models most frequently used in practical calculations: i) For the Gilber-Cameron model the parameters of the Beijing group, based on a rather recent compilations of the neutron resonance and low-lying level densities and included into the beijing-gc.dat file, are chosen as recommended. As alternative versions the parameters provided by other groups are given into the files: jaeri-gc.dat, bombay-gc.dat, obninsk-gc.dat. Additionally the iljinov-gc.dat, and mengoni-gc.dat files include sets of the level density parameters that take into account the damping of shell effects at high energies. ii) For the backed-shifted Fermi gas model the beijing-bs.dat file is selected as the recommended one. Alternative parameters of the Obninsk group are given in the obninsk-bs.dat file and those of Bombay in bombay-bs.dat. iii) For the generalized superfluid model the Obninsk group parameters included into the obninsk-bcs.dat file are chosen as recommended ones and the beijing-bcs.dat file is included as an alternative set of parameters. iv) For the microscopic approach to the level densities the files are: obninsk-micro.for -FORTRAN 77 source for the microscopical statistical level density code developed in Obninsk by Ignatyuk and coworkers, moller-levels.gz - Moeller single-particle level and ground state deformation data base, moller-levels.for -retrieval code for Moeller single-particle level scheme. (author)

  12. Wireless connection of continuous glucose monitoring system to the electronic patient record

    Science.gov (United States)

    Murakami, Alexandre; Gutierrez, Marco A.; Lage, Silvia G.; Rebelo, Marina S.; Granja, Luiz A. R.; Ramires, Jose A. F.

    2005-04-01

    The control of blood sugar level (BSL) at near-normal levels has been documented to reduce both acute and chronic complications of diabetes mellitus. Recent studies suggested, the reduction of mortality in a surgical intensive care unit (ICU), when the BSL are maintained at normal levels. Despite of the benefits appointed by these and others clinical studies, the strict BSL control in critically ill patients suffers from some difficulties: a) medical staff need to measure and control the patient"s BSL using blood sample at least every hour. This is a complex and time consuming task; b) the inaccuracy of standard capillary glucose monitoring (fingerstick) in hypotensive patients and, if frequently used to sample arterial or venous blood, may lead to excess phlebotomy; c) there is no validated procedure for continuously monitoring of BSL levels. This study used the MiniMed CGMS in ill patients at ICU to send, in real-time, BSL values to a Web-Based Electronic Patient Record. The BSL values are parsed and delivered through a wireless network as an HL7 message. The HL7 messages with BSL values are collected, stored into the Electronic Patient Record and presented into a bed-side monitor at the ICU together with other relevant patient information.

  13. Laboratory Activity to Teach about the Proliferation of Salmonella in Vegetables

    Directory of Open Access Journals (Sweden)

    Massimiliano Marvasi

    2015-08-01

    Recommendations: Introductory Course in Microbiology and the Guidelines for Biosafety in Teaching Laboratories, available at www.asm.org. The Editors of JMBE recommend that adopters of the protocols included in this article follow a minimum of Biosafety Level 2 practices.

  14. The Convergence of High-Consequence Livestock and Human Pathogen Research and Development: A Paradox of Zoonotic Disease

    Directory of Open Access Journals (Sweden)

    Julia M. Michelotti

    2018-05-01

    Full Text Available The World Health Organization (WHO estimates that zoonotic diseases transmitted from animals to humans account for 75 percent of new and emerging infectious diseases. Globally, high-consequence pathogens that impact livestock and have the potential for human transmission create research paradoxes and operational challenges for the high-containment laboratories that conduct work with them. These specialized facilities are required for conducting all phases of research on high-consequence pathogens (basic, applied, and translational with an emphasis on both the generation of fundamental knowledge and product development. To achieve this research mission, a highly-trained workforce is required and flexible operational methods are needed. In addition, working with certain pathogens requires compliance with regulations such as the Centers for Disease Control (CDC and the U.S. Department of Agriculture (USDA Select Agent regulations, which adds to the operational burden. The vast experience from the existing studies at Plum Island Animal Disease Center, other U.S. laboratories, and those in Europe and Australia with biosafety level 4 (BSL-4 facilities designed for large animals, clearly demonstrates the valuable contribution this capability brings to the efforts to detect, prepare, prevent and respond to livestock and potential zoonotic threats. To raise awareness of these challenges, which include biosafety and biosecurity issues, we held a workshop at the 2018 American Society for Microbiology (ASM Biothreats conference to further discuss the topic with invited experts and audience participants. The workshop covered the subjects of research funding and metrics, economic sustainment of drug and vaccine development pipelines, workforce turnover, and the challenges of maintaining operational readiness of high containment laboratories.

  15. 生物安全議定書下的事前告知同意程序與WTO之SPS協定中有關動植物進口程序的關係 ― 兼論台灣與議定書締約國訂定LMOs之跨境運輸協定 An Analysis on the relationship between sanitary and phytosanitary import procedures under the SPS Agreement of the WTO and the Advanced Informed Agreement procedures of the Biosafety Protocol

    Directory of Open Access Journals (Sweden)

    施文真 Yu Wen-Chen Shih

    2004-06-01

    sanitary and phytosanitary measures to protect the domestic environment. The compatibility of these two sets of import control procedures hinges on the relationship between international environmental agreement and the WTO. The level of import control is also related to how a government plans to balance the interests of protecting its domestic environment and promoting the development of biotechnology. All these issues are highly controversial and attracted keen debate amongst scholars and practitioners. This paper will focus on the procedural design of these two sets of import control system and seek to avoid potential conflict between these two regimes by proposing a set of procedures that can meet the requirements under these two regimes. Meanwhile, the Biosafety Protocol authorizes Parties to conclude bilateral, regional or multilateral agreements with non-Parties concerning the transboundary movement of LMOs. Taiwan is not a Party to the Protocol. This paper, thus, will discuss possible strategies Taiwan may adopt when facing such negotiation.

  16. Pathogens Inactivated by Low-Energy-Electron Irradiation Maintain Antigenic Properties and Induce Protective Immune Responses

    Science.gov (United States)

    Fertey, Jasmin; Bayer, Lea; Grunwald, Thomas; Pohl, Alexandra; Beckmann, Jana; Gotzmann, Gaby; Casado, Javier Portillo; Schönfelder, Jessy; Rögner, Frank-Holm; Wetzel, Christiane; Thoma, Martin; Bailer, Susanne M.; Hiller, Ekkehard; Rupp, Steffen; Ulbert, Sebastian

    2016-01-01

    Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone. This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures are often required. Moreover, application of chemicals alters the antigenic components of the viruses or bacteria, resulting in reduced antibody specificity and therefore stimulation of a less effective immune response. An alternative method for inactivation of pathogens is ionizing radiation. It acts very fast and predominantly damages nucleic acids, conserving most of the antigenic structures. However, currently used irradiation technologies (mostly gamma-rays and high energy electrons) require large and complex shielding constructions to protect the environment from radioactivity or X-rays generated during the process. This excludes them from direct integration into biological production facilities. Here, low-energy electron irradiation (LEEI) is presented as an alternative inactivation method for pathogens in liquid solutions. LEEI can be used in normal laboratories, including good manufacturing practice (GMP)- or high biosafety level (BSL)-environments, as only minor shielding is necessary. We show that LEEI efficiently inactivates different viruses (influenza A (H3N8), porcine reproductive and respiratory syndrome virus (PRRSV), equine herpesvirus 1 (EHV-1)) and bacteria (Escherichia coli) and maintains their antigenicity. Moreover, LEEI-inactivated influenza A viruses elicit protective immune responses in animals, as analyzed by virus neutralization assays and viral load determination upon challenge. These results have implications for novel ways of developing and manufacturing inactivated vaccines with improved efficacy. PMID:27886076

  17. The pathogenecity of H5N1 highly pathogenic Avian Influenza (HPAI virus clade 2.3.2. in Indonesian indigenous chicken by contact tranmission with infected duck

    Directory of Open Access Journals (Sweden)

    R. Damayanti

    2017-05-01

    Full Text Available An experimental transmission study was conducted using nine healthy Indonesian indigenous chickens placed together with two 30 days old ducks which were experimentally infected with H5N1 HPAI clade 2.3.2 virus in the Biosafety Laboratory Level 3 (BSL-3 facilities. The aim of the study was to find out the pathogenicity of H5N1 HPAI virus clade 2.3.2 in Indonesian indigenous chickens. The study showed that within twenty four hours rearing, the chickens were exhibited mild clinical signs and by 48 hours, all of the chickens died, whereas the ducks survived but with severe clinical signs. The H5N1 HPAI virus has been successfully isolated from chickens and ducks swabs, confirming that those animals were infected by the virus. Histologically, the infected chicken encountered with severe inflammation reaction namely non suppuratives encephalitis, tracheitis, myocarditis, interstitial pneumonia, hepatitis, proventriculitis, enteritis, pancreatitis, nephritis and bursitis. Necrotizing spleen and pancreas were also prominent. Viral antigen was detected by immunohistochemistry staining in various affected visceral organs. This suggests that Indonesian indigenous chickens were susceptible to H5N1 HPAI virus clade 2.3.2 and it can be transmitted easily to Indonesian indigenous chickens by contact transmission with infected ducks.

  18. National Laboratory Planning: Developing Sustainable Biocontainment Laboratories in Limited Resource Areas.

    Science.gov (United States)

    Yeh, Kenneth B; Adams, Martin; Stamper, Paul D; Dasgupta, Debanjana; Hewson, Roger; Buck, Charles D; Richards, Allen L; Hay, John

    2016-01-01

    Strategic laboratory planning in limited resource areas is essential for addressing global health security issues. Establishing a national reference laboratory, especially one with BSL-3 or -4 biocontainment facilities, requires a heavy investment of resources, a multisectoral approach, and commitments from multiple stakeholders. We make the case for donor organizations and recipient partners to develop a comprehensive laboratory operations roadmap that addresses factors such as mission and roles, engaging national and political support, securing financial support, defining stakeholder involvement, fostering partnerships, and building trust. Successful development occurred with projects in African countries and in Azerbaijan, where strong leadership and a clear management framework have been key to success. A clearly identified and agreed management framework facilitate identifying the responsibility for developing laboratory capabilities and support services, including biosafety and biosecurity, quality assurance, equipment maintenance, supply chain establishment, staff certification and training, retention of human resources, and sustainable operating revenue. These capabilities and support services pose rate-limiting yet necessary challenges. Laboratory capabilities depend on mission and role, as determined by all stakeholders, and demonstrate the need for relevant metrics to monitor the success of the laboratory, including support for internal and external audits. Our analysis concludes that alternative frameworks for success exist for developing and implementing capabilities at regional and national levels in limited resource areas. Thus, achieving a balance for standardizing practices between local procedures and accepted international standards is a prerequisite for integrating new facilities into a country's existing public health infrastructure and into the overall international scientific community.

  19. Genistein, a general kinase inhibitor, as a potential antiviral for arenaviral hemorrhagic fever as described in the Pirital virus-Syrian golden hamster model.

    Science.gov (United States)

    Vela, Eric M; Knostman, Katherine A; Mott, Jason M; Warren, Richard L; Garver, Jennifer N; Vela, Lela Johnson; Stammen, Rachelle L

    2010-09-01

    Arenaviruses are rodent-borne negative strand RNA viruses and infection of these viruses in humans may result in disease and hemorrhagic fever. To date, supportive care, ribavirin, and in some cases immune plasma remain the foremost treatment options for arenaviral hemorrhagic fever. Research with the hemorrhagic fever causing-arenaviruses usually requires a Biosafety level (BSL)-4 environment; however, surrogate animal model systems have been developed to preliminarily study and screen various vaccines and antivirals. The Syrian golden hamster-Pirital virus (PIRV) surrogate model of hemorrhagic fever provides an opportunity to test new antivirals in an ABSL-3 setting. Thus, we challenged hamsters, implanted with telemetry, with PIRV and observed viremia and tissue viral titers, and changes in core body temperature, hematology, clinical chemistry, and coagulation parameters. Physical signs of disease of the PIRV-infected hamsters included weight loss, lethargy, petechial rashes, epistaxis, ocular orbital and rectal hemorrhage, and visible signs of neurologic disorders. However, treating animals with genistein, a plant derived isoflavone and general kinase inhibitor, resulted in increased survival rates and led to an improved clinical profile. In all, the results from this study demonstrate the potential of a general kinase inhibitor genistein as an antiviral against arenaviral hemorrhagic fever. 2010 Elsevier B.V. All rights reserved.

  20. High Intensity High Volume Interval Training Improves Endurance Performance and Induces a Nearly Complete Slow-to-Fast Fiber Transformation on the mRNA Level

    Directory of Open Access Journals (Sweden)

    Julian Eigendorf

    2018-05-01

    Full Text Available We present here a longitudinal study determining the effects of two 3 week-periods of high intensity high volume interval training (HIHVT (90 intervals of 6 s cycling at 250% maximum power, Pmax/24 s on a cycle ergometer. HIHVT was evaluated by comparing performance tests before and after the entire training (baseline, BSL, and endpoint, END and between the two training sets (intermediate, INT. The mRNA expression levels of myosin heavy chain (MHC isoforms and markers of energy metabolism were analyzed in M. vastus lateralis biopsies by quantitative real-time PCR. In incremental tests peak power (Ppeak was increased, whereas V˙O2peak was unaltered. Prolonged time-to-exhaustion was found in endurance tests with 65 and 80% Pmax at INT and END. No changes in blood levels of lipid metabolites were detected. Training-induced decreases of hematocrit indicate hypervolemia. A shift from slow MHCI/β to fast MHCIIa mRNA expression occurred after the first and second training set. The mRNA expression of peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α, a master regulator of oxidative energy metabolism, decreased after the second training set. In agreement, a significant decrease was also found for citrate synthase mRNA after the second training set, indicating reduced oxidative capacity. However, mRNA expression levels of glycolytic marker enzyme glyceraldehyde-3-phosphate dehydrogenase did not change after the first and second training set. HIHVT induced a nearly complete slow-to-fast fiber type transformation on the mRNA level, which, however, cannot account for the improvements of performance parameters. The latter might be explained by the well-known effects of hypervolemia on exercise performance.

  1. Rapid screening for entry inhibitors of highly pathogenic viruses under low-level biocontainment.

    Directory of Open Access Journals (Sweden)

    Aparna Talekar

    Full Text Available Emerging viruses including Nipah, Hendra, Lujo, and Junin viruses have enormous potential to spread rapidly. Nipah virus, after emerging as a zoonosis, has also evolved the capacity for human-to-human transmission. Most of the diseases caused by these pathogens are untreatable and require high biocontainment conditions. Universal methods for rapidly identifying and screening candidate antivirals are urgently needed. We have developed a modular antiviral platform strategy that relies on simple bioinformatic and genetic information about each pathogen. Central to this platform is the use of envelope glycoprotein cDNAs to establish multi-cycle replication systems under BSL2 conditions for viral pathogens that normally require BSL3 and BSL4 facilities. We generated monoclonal antibodies against Nipah G by cDNA immunization in rats, and we showed that these antibodies neutralize both Nipah and Hendra live viruses. We then used these effective Henipavirus inhibitors to validate our screening strategy. Our proposed strategy should contribute to the response capability for emerging infectious diseases, providing a way to initiate antiviral development immediately upon identifying novel viruses.

  2. Interactions between proteins and carbon-based nanoparticles: exploring the origin of nanotoxicity at the molecular level.

    Science.gov (United States)

    Zuo, Guanghong; Kang, Seung-Gu; Xiu, Peng; Zhao, Yuliang; Zhou, Ruhong

    2013-05-27

    The widespread application of nanomaterials has spurred an interest in the study of interactions between nanoparticles and proteins due to the biosafety concerns of these nanomaterials. In this review, a summary is presented of some of the recent studies on this important subject, especially on the interactions of proteins with carbon nanotubes (CNTs) and metallofullerenols. Two potential molecular mechanisms have been proposed for CNTs' inhibition of protein functions. The driving forces of CNTs' adsorption onto proteins are found to be mainly hydrophobic interactions and the so-called π-π stacking between CNTs' carbon rings and proteins' aromatic residues. However, there is also recent evidence showing that endohedral metallofullerenol Gd@C82 (OH)22 can be used to inhibit tumor growth, thus acting as a potential nanomedicine. These recent findings have provided a better understanding of nanotoxicity at the molecular level and also suggested therapeutic potential by using nanoparticles' cytotoxicity against cancer cells. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides

    Directory of Open Access Journals (Sweden)

    Bruce D. Hammock

    2007-06-01

    Full Text Available The entomopathogenic Autographa californica (Speyer nucleopolyhedrovirus (AcMNPV has been genetically modified to increase its speed of kill. The potential adverse effects of a recombinant AcMNPV (AcAaIT as well as wild type AcMNPV and wild type Spodoptera littoralis NPV (SlNPV were studied. Cotton plants were treated with these viruses at concentrations that were adjusted to resemble the recommended field application rate (4 x 1012 PIBs/feddan, feddan = 4,200 m2 and 3rd instar larvae of S. littoralis were allowed to feed on the contaminated plants. SDS-PAGE, ELISA, and DNA analyses were used to confirm that larvae that fed on these plants were virus-infected. Polyhedra that were purified from the infected larvae were subjected to structural protein analysis. A 32 KDa protein was found in polyhedra that were isolated from all of the viruses. Subtle differences were found in the size and abundance of ODV proteins. Antisera against polyhedral proteins isolated from AcAaIT polyhedra were raised in rabbits. The terminal bleeds from rabbits were screened against four coating antigens (i.e., polyhedral proteins from AcAaIT, AcAaIT from field-infected larvae (AcAaIT-field, AcMNPV, and SlNPV using a two-dimensional titration method with the coated antigen format. Competitive inhibition experiments were conducted in parallel to optimize antibody and coating antigen concentrations for ELISA. The IC50 values for each combination ranged from 1.42 to 163 μg/ml. AcAaIT-derived polyhedrin gave the lowest IC50 value, followed by those of SlNPV, AcAaIT-field, and AcMNPV. The optimized ELISA system showed low cross reactivity for AcMNPV (0.87%, AcAaIT-field (1.2%, and SlNPV (4.0%. Genomic DNAs isolated from AcAaIT that were passaged in larvae of S. littoralis that were reared in the laboratory or field did not show any detectable differences. Albino rats (male and female that were treated with AcAaIT, AcMNPV or SlNPV (either orally or by intraperitoneal injection at doses of 1 x 108 or 1 x 107 PIBs/rat, respectively appeared to be healthy and showed increased body weight at 21 days posttreatment. The effect of virus administration on hematological, serum biochemical, and histopathological parameters were determined. Slight to moderate differences were observed in most of the hematological parameters. Specifically, serum proteins were decreased markedly in female rats treated orally with SlNPV, and in male rats injected with AcAaIT. SDS-PAGE analysis also showed some changes in serum protein profiles. No marked changes in acetylcholine esterase (AChE activity were found. Changes in serum glucose, alanine aminotransferase (ALT, aspartate aminotransferase (AST, alkaline phosphatase (ALP, bilirubin, creatinin, and urea were also observed. Immunohistochemical observation of tissues from stomach, intestine, liver, kidney, brain, spleen, and lung also showed slight changes. Fish (Tilapia nilotica were also exposed to AcAaIT, AcMNPV or SlNPV by incorporating each of the viruses into diet (1 x 109 PIBs/group. No mortality was found in treated or untreated fish during the experimental period (28 days. Macrophage phagocytic activity of fish head kidney cells increased with time, reaching maximum values at 180 min for both treated and control fish.

  4. Biosafety Management of Genetically Modified Crops (China) | CRDI ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Since 1990, China's agricultural biotechnology sector has experienced tremendous growth. A recent survey shows that the country is developing the largest plant biotechnology capacity outside North America. Public investment in the sector, as well as the number of genetically modified (GM) crops commercialized, ...

  5. Laboratory Biosafety and Biosecurity Risk Assessment Technical Guidance Document

    Energy Technology Data Exchange (ETDEWEB)

    Astuto-Gribble, Lisa M; Caskey, Susan Adele

    2014-07-01

    The purpose of this document is threefold: 1) to describe the laboratory bio safety and biosecurity risk assessment process and its conceptual framework; 2) provide detailed guidance and suggested methodologies on how to conduct a risk assessment; and 3) present some practical risk assessment process strategies using realistic laboratory scenarios.

  6. Stress-induced evolution and the biosafety of genetically modified

    Indian Academy of Sciences (India)

    This article is focused on the problems of reduction of the risk associated with the deliberate release of genetically modified microorganisms (GMMs) into the environment. Special attention is given to overview the most probable physiological and genetic processes which could be induced in the released GMMs by adverse ...

  7. Education on biosafety and bioethics: necessary articulation in biotechnology

    OpenAIRE

    Bonis, Marcos De; Costa, Marco Antonio Ferreira da

    2009-01-01

    A educação científica tem sido debatida em alguns segmentos da sociedade e organizações internacionais têm estimulado as nações a investirem nessa área tão estratégica. Nesse contexto, a educação em biossegurança e bioética explora um conteúdo rico em prevenção, normas e princípios éticos, que servem para nortear os caminhos trilhados pela biotecnologia. A valorização da biossegurança e da bioética como parte de uma política educacional científica, efetiva e consistente, pode estimular a form...

  8. Role of institutional biosafety committees in Kenya | Wafula | African ...

    African Journals Online (AJOL)

    African Crop Science Journal. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 3, No 3 (1995) >. Log in or Register to get access to full text downloads.

  9. Biosafety regulations in Brazil | Sampaio | African Crop Science ...

    African Journals Online (AJOL)

    African Crop Science Journal. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 3, No 3 (1995) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Download this PDF file. The PDF file you selected should ...

  10. Behavior of lactobacilli isolated from fermented slurry (ben-saalga in gnotobiotic rats.

    Directory of Open Access Journals (Sweden)

    Williams Turpin

    Full Text Available Most bacterial strains, which have been studied so far for their probiotic functions, are extensively used by manufacturers in developed countries. In our work, we sought to study a mix (called BSL comprising three strains belonging to Lactobacillus fermentum, L. paraplantarum and L. salivarius, that were isolated from a traditional African pearl millet based fermented slurry. Our objective was to study this BSL cocktail in gnotobiotic rats, to evaluate their survival and their behavior in the digestive tract conditions. After a single oral inoculation of germfree rats with BSL, the species established stably in the digestive tract with the following hierarchy of abundance: L. salivarius> L. plantarum> L. fermentum. BSL cocktail was metabolically active since it produced 50 mM lactate and it expressed genes involved in binding mechanism in the caecum. Furthermore, the global morphology of the colon epithelium was not disturbed by the BSL cocktail. BSL cocktail did not modify mucus content and host mucus-related genes (MUC1, MUC2, MUC3 or resistin-like molecule β. The cocktail of lactobacilli enhanced the proliferating cell nuclear antigen (PCNA at a level comparable to what was observed in conventional rats. PCNA was involved in proliferation and DNA repair, but the presence of the cocktail did not provoke proliferative events (with Ki67 as indicator, so we suppose BSL may help gut preservation. This work is the first step towards the selection of strains that are derived from traditional fermented food to formulate new probiotic mixture.

  11. A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System

    Directory of Open Access Journals (Sweden)

    Broder Christopher C

    2010-11-01

    Full Text Available Abstract Background Hendra virus (HeV and Nipah virus (NiV are newly emerged zoonotic paramyxoviruses discovered during outbreaks in Queensland, Australia in 1994 and peninsular Malaysia in 1998/9 respectively and classified within the new Henipavirus genus. Both viruses can infect a broad range of mammalian species causing severe and often-lethal disease in humans and animals, and repeated outbreaks continue to occur. Extensive laboratory studies on the host cell infection stage of HeV and NiV and the roles of their envelope glycoproteins have been hampered by their highly pathogenic nature and restriction to biosafety level-4 (BSL-4 containment. To circumvent this problem, we have developed a henipavirus envelope glycoprotein pseudotyped lentivirus assay system using either a luciferase gene or green fluorescent protein (GFP gene encoding human immunodeficiency virus type-1 (HIV-1 genome in conjunction with the HeV and NiV fusion (F and attachment (G glycoproteins. Results Functional retrovirus particles pseudotyped with henipavirus F and G glycoproteins displayed proper target cell tropism and entry and infection was dependent on the presence of the HeV and NiV receptors ephrinB2 or B3 on target cells. The functional specificity of the assay was confirmed by the lack of reporter-gene signals when particles bearing either only the F or only G glycoprotein were prepared and assayed. Virus entry could be specifically blocked when infection was carried out in the presence of a fusion inhibiting C-terminal heptad (HR-2 peptide, a well-characterized, cross-reactive, neutralizing human mAb specific for the henipavirus G glycoprotein, and soluble ephrinB2 and B3 receptors. In addition, the utility of the assay was also demonstrated by an examination of the influence of the cytoplasmic tail of F in its fusion activity and incorporation into pseudotyped virus particles by generating and testing a panel of truncation mutants of NiV and HeV F

  12. A novel life cycle modeling system for Ebola virus shows a genome length-dependent role of VP24 in virus infectivity.

    Science.gov (United States)

    Watt, Ari; Moukambi, Felicien; Banadyga, Logan; Groseth, Allison; Callison, Julie; Herwig, Astrid; Ebihara, Hideki; Feldmann, Heinz; Hoenen, Thomas

    2014-09-01

    Work with infectious Ebola viruses is restricted to biosafety level 4 (BSL4) laboratories, presenting a significant barrier for studying these viruses. Life cycle modeling systems, including minigenome systems and transcription- and replication-competent virus-like particle (trVLP) systems, allow modeling of the virus life cycle under BSL2 conditions; however, all current systems model only certain aspects of the virus life cycle, rely on plasmid-based viral protein expression, and have been used to model only single infectious cycles. We have developed a novel life cycle modeling system allowing continuous passaging of infectious trVLPs containing a tetracistronic minigenome that encodes a reporter and the viral proteins VP40, VP24, and GP1,2. This system is ideally suited for studying morphogenesis, budding, and entry, in addition to genome replication and transcription. Importantly, the specific infectivity of trVLPs in this system was ∼ 500-fold higher than that in previous systems. Using this system for functional studies of VP24, we showed that, contrary to previous reports, VP24 only very modestly inhibits genome replication and transcription when expressed in a regulated fashion, which we confirmed using infectious Ebola viruses. Interestingly, we also discovered a genome length-dependent effect of VP24 on particle infectivity, which was previously undetected due to the short length of monocistronic minigenomes and which is due at least partially to a previously unknown function of VP24 in RNA packaging. Based on our findings, we propose a model for the function of VP24 that reconciles all currently available data regarding the role of VP24 in nucleocapsid assembly as well as genome replication and transcription. Ebola viruses cause severe hemorrhagic fevers in humans, with no countermeasures currently being available, and must be studied in maximum-containment laboratories. Only a few of these laboratories exist worldwide, limiting our ability to study

  13. Sea level change

    Digital Repository Service at National Institute of Oceanography (India)

    Church, J.A.; Clark, P.U.; Cazenave, A.; Gregory, J.M.; Jevrejeva, S.; Levermann, A.; Merrifield, M.A.; Milne, G.A.; Nerem, R.S.; Nunn, P.D.; Payne, A.J.; Pfeffer, W.T.; Stammer, D.; Unnikrishnan, A.S.

    This chapter considers changes in global mean sea level, regional sea level, sea level extremes, and waves. Confidence in projections of global mean sea level rise has increased since the Fourth Assessment Report (AR4) because of the improved...

  14. Predicate Structures, Gesture, and Simultaneity in the Representation of Action in British Sign Language: Evidence From Deaf Children and Adults

    Science.gov (United States)

    Cormier, Kearsy

    2013-01-01

    British Sign Language (BSL) signers use a variety of structures, such as constructed action (CA), depicting constructions (DCs), or lexical verbs, to represent action and other verbal meanings. This study examines the use of these verbal predicate structures and their gestural counterparts, both separately and simultaneously, in narratives by deaf children with various levels of exposure to BSL (ages 5;1 to 7;5) and deaf adult native BSL signers. Results reveal that all groups used the same types of predicative structures, including children with minimal BSL exposure. However, adults used CA, DCs, and/or lexical signs simultaneously more frequently than children. These results suggest that simultaneous use of CA with lexical and depicting predicates is more complex than the use of these predicate structures alone and thus may take deaf children more time to master. PMID:23670881

  15. Governança global e transferência de política: influências do Protocolo de Cartagena na Política Nacional de Biossegurança Gobernanza global y transferencia de la política: influencias del Protocolo de Cartagena en la Política Nacional de Bioseguridad Global governance and transfer policy: influences of the Cartagena Protocol on Brazilian National Policy on Biosafety

    Directory of Open Access Journals (Sweden)

    Yuna Fontoura

    2013-02-01

    Full Text Available No contexto de governança global, analisamos o tema da transferência de política, no qual a formulação de políticas públicas é influenciada por experiências de contextos políticos diferentes. Neste sentido, questionamos de que forma o Protocolo de Cartagena influenciou a formulação da Política Nacional de Biossegurança (PNB. A pesquisa empírica foi realizada por meio de entrevistas semiestruturadas com respondentes qualificados, que atuaram direta ou indiretamente na formulação da PNB. No tratamento dos dados adotamos uma abordagem qualitativa, por meio da utilização da análise de conteúdo. Os resultados revelam que houve transferência de política no formato de aprendizado (ou lesson drawing do Protocolo de Cartagena à PNB.En el contexto de la gobernancia global, se analiza el tema de la política de transferencia, en que la formulación de la política pública está influenciada por las experiencias de los diferentes contextos políticos. En este sentido, nos preguntamos cómo el Protocolo de Cartagena ha influido en la formulación de la Política Nacional de Bioseguridad (PNB. La investigación empírica se realizó a través de entrevistas semi-estructuradas con informantes calificados, que trabajaban directa o indirectamente en la formulación de la PNB. En el procesamiento de los datos seguimos un enfoque cualitativo, a través del uso de análisis de contenido. Los resultados muestran que ocorre una política de transferencia en la forma de aprendizaje (o lesson drawing del Protocolo de Cartagena sobre PNB.In the context of global governance, we analyze the topic of policy transfer in which the formulation of public policies is influenced by experiences of different political contexts. Thus, we question how the Cartagena Protocol influenced the formulation of the Brazilian National Biosafety Policy (PNB. The empirical research was accomplished through semi-structured interviews with qualified respondents who

  16. Guidance levels, achievable doses and expectation levels

    International Nuclear Information System (INIS)

    Li, Lianbo; Meng, Bing

    2002-01-01

    The National Radiological Protection Board (NRPB), the International Atomic Energy Agency (IAEA) and the Commission of the European Communities (CEC) published their guidance levels and reference doses for typical X-ray examination and nuclear medicine in their documents in 1993, 1994 and 1996 respectively. From then on, the concept of guidance levels or reference doses have been applied to different examinations in the field of radiology and proved to be effective for reduction of patient doses. But the guidance levels or reference doses are likely to have some shortcomings and can do little to make further reduction of patient dose in the radiology departments where patient dose are already below them. For this reason, the National Radiological Protection Board (NRPB) proposed a concept named achievable doses which are based on the mean dose observed for a selected sample of radiology departments. This paper will review and discuss the concept of guidance levels and achievable doses, and propose a new concept referred to as Expectation Levels that will encourage the radiology departments where patient dose are already below the guidance levels to keep patient dose as low as reasonably achievable. Some examples of the expectation levels based on the data published by a few countries are also illustrated in this paper

  17. Readability versus Leveling.

    Science.gov (United States)

    Fry, Edward

    2002-01-01

    Shows some similarities and differences between readability formulas and leveling procedures and reports some current large-scale uses of readability formulas. Presents a dictionary definition of readability and leveling, and a history and background of readability and leveling. Discusses what goes into determining readability and leveling scores.…

  18. Ubiquitin-regulated nuclear-cytoplasmic trafficking of the Nipah virus matrix protein is important for viral budding.

    Directory of Open Access Journals (Sweden)

    Yao E Wang

    2010-11-01

    Full Text Available Paramyxoviruses are known to replicate in the cytoplasm and bud from the plasma membrane. Matrix is the major structural protein in paramyxoviruses that mediates viral assembly and budding. Curiously, the matrix proteins of a few paramyxoviruses have been found in the nucleus, although the biological function associated with this nuclear localization remains obscure. We report here that the nuclear-cytoplasmic trafficking of the Nipah virus matrix (NiV-M protein and associated post-translational modification play a critical role in matrix-mediated virus budding. Nipah virus (NiV is a highly pathogenic emerging paramyxovirus that causes fatal encephalitis in humans, and is classified as a Biosafety Level 4 (BSL4 pathogen. During live NiV infection, NiV-M was first detected in the nucleus at early stages of infection before subsequent localization to the cytoplasm and the plasma membrane. Mutations in the putative bipartite nuclear localization signal (NLS and the leucine-rich nuclear export signal (NES found in NiV-M impaired its nuclear-cytoplasmic trafficking and also abolished NiV-M budding. A highly conserved lysine residue in the NLS served dual functions: its positive charge was important for mediating nuclear import, and it was also a potential site for monoubiquitination which regulates nuclear export of the protein. Concordantly, overexpression of ubiquitin enhanced NiV-M budding whereas depletion of free ubiquitin in the cell (via proteasome inhibitors resulted in nuclear retention of NiV-M and blocked viral budding. Live Nipah virus budding was exquisitely sensitive to proteasome inhibitors: bortezomib, an FDA-approved proteasome inhibitor for treating multiple myeloma, reduced viral titers with an IC(50 of 2.7 nM, which is 100-fold less than the peak plasma concentration that can be achieved in humans. This opens up the possibility of using an "off-the-shelf" therapeutic against acute NiV infection.

  19. Animal models for Ebola and Marburg virus infections

    Science.gov (United States)

    Nakayama, Eri; Saijo, Masayuki

    2013-01-01

    Ebola and Marburg hemorrhagic fevers (EHF and MHF) are caused by the Filoviridae family, Ebolavirus and Marburgvirus (ebolavirus and marburgvirus), respectively. These severe diseases have high mortality rates in humans. Although EHF and MHF are endemic to sub-Saharan Africa. A novel filovirus, Lloviu virus, which is genetically distinct from ebolavirus and marburgvirus, was recently discovered in Spain where filoviral hemorrhagic fever had never been reported. The virulence of this virus has not been determined. Ebolavirus and marburgvirus are classified as biosafety level-4 (BSL-4) pathogens and Category A agents, for which the US government requires preparedness in case of bioterrorism. Therefore, preventive measures against these viral hemorrhagic fevers should be prepared, not only in disease-endemic regions, but also in disease-free countries. Diagnostics, vaccines, and therapeutics need to be developed, and therefore the establishment of animal models for EHF and MHF is invaluable. Several animal models have been developed for EHF and MHF using non-human primates (NHPs) and rodents, which are crucial to understand pathophysiology and to develop diagnostics, vaccines, and therapeutics. Rhesus and cynomolgus macaques are representative models of filovirus infection as they exhibit remarkably similar symptoms to those observed in humans. However, the NHP models have practical and ethical problems that limit their experimental use. Furthermore, there are no inbred and genetically manipulated strains of NHP. Rodent models such as mouse, guinea pig, and hamster, have also been developed. However, these rodent models require adaptation of the virus to produce lethal disease and do not mirror all symptoms of human filovirus infection. This review article provides an outline of the clinical features of EHF and MHF in animals, including humans, and discusses how the animal models have been developed to study pathophysiology, vaccines, and therapeutics. PMID:24046765

  20. Animal models for Ebola and Marburg virus infections

    Directory of Open Access Journals (Sweden)

    Eri eNakayama

    2013-09-01

    Full Text Available Ebola and Marburg hemorrhagic fevers (EHF and MHF are caused by the Filoviridae family, Ebolavirus and Marburgvirus (ebolavirus and marburgvirus, respectively. These severe diseases have high mortality rates in humans. Although EHF and MHF are endemic to sub-Saharan Africa. A novel filovirus, Lloviu virus, which is genetically distinct from ebolavirus and marburgvirus, was recently discovered in Spain where filoviral hemorrhagic fever had never been reported. The virulence of this virus has not been determined. Ebolavirus and marburgvirus are classified as biosafety level-4 (BSL-4 pathogens and Category A agents, for which the US government requires preparedness in case of bioterrorism. Therefore, preventive measures against these viral hemorrhagic fevers should be prepared, not only in disease-endemic regions, but also in disease-free countries. Diagnostics, vaccines, and therapeutics need to be developed, and therefore the establishment of animal models for EHF and MHF is invaluable. Several animal models have been developed for EHF and MHF using nonhuman primates (NHPs and rodents, which are crucial to understand pathophysiology and to develop diagnostics, vaccines, and therapeutics. Rhesus and cynomolgus macaques are representative models of filovirus infection as they exhibit remarkably similar symptoms to those observed in humans. However, the NHP models have practical and ethical problems that limit their experimental use. Furthermore, there are no inbred and genetically manipulated strains of NHP. Rodent models such as mouse, guinea pig, and hamster, have also been developed. However, these rodent models require adaptation of the virus to produce lethal disease and do not mirror all symptoms of human filovirus infection. This review article provides an outline of the clinical features of EHF and MHF in animals, including humans, and discusses how the animal models have been developed to study pathophysiology, vaccines, and therapeutics.

  1. Validation of the Filovirus Plaque Assay for Use in Preclinical Studies

    Directory of Open Access Journals (Sweden)

    Amy C. Shurtleff

    2016-04-01

    Full Text Available A plaque assay for quantitating filoviruses in virus stocks, prepared viral challenge inocula and samples from research animals has recently been fully characterized and standardized for use across multiple institutions performing Biosafety Level 4 (BSL-4 studies. After standardization studies were completed, Good Laboratory Practices (GLP-compliant plaque assay method validation studies to demonstrate suitability for reliable and reproducible measurement of the Marburg Virus Angola (MARV variant and Ebola Virus Kikwit (EBOV variant commenced at the United States Army Medical Research Institute of Infectious Diseases (USAMRIID. The validation parameters tested included accuracy, precision, linearity, robustness, stability of the virus stocks and system suitability. The MARV and EBOV assays were confirmed to be accurate to ±0.5 log10 PFU/mL. Repeatability precision, intermediate precision and reproducibility precision were sufficient to return viral titers with a coefficient of variation (%CV of ≤30%, deemed acceptable variation for a cell-based bioassay. Intraclass correlation statistical techniques for the evaluation of the assay’s precision when the same plaques were quantitated by two analysts returned values passing the acceptance criteria, indicating high agreement between analysts. The assay was shown to be accurate and specific when run on Nonhuman Primates (NHP serum and plasma samples diluted in plaque assay medium, with negligible matrix effects. Virus stocks demonstrated stability for freeze-thaw cycles typical of normal usage during assay retests. The results demonstrated that the EBOV and MARV plaque assays are accurate, precise and robust for filovirus titration in samples associated with the performance of GLP animal model studies.

  2. Data analysis at Level-1 Trigger level

    CERN Document Server

    Wittmann, Johannes; Aradi, Gregor; Bergauer, Herbert; Jeitler, Manfred; Wulz, Claudia; Apanasevich, Leonard; Winer, Brian; Puigh, Darren Michael

    2017-01-01

    With ever increasing luminosity at the LHC, optimum online data selection is getting more and more important. While in the case of some experiments (LHCb and ALICE) this task is being completely transferred to computer farms, the others - ATLAS and CMS - will not be able to do this in the medium-term future for technological, detector-related reasons. Therefore, these experiments pursue the complementary approach of migrating more and more of the offline and High-Level Trigger intelligence into the trigger electronics. This paper illustrates how the Level-1 Trigger of the CMS experiment and in particular its concluding stage, the Global Trigger, take up this challenge.

  3. Low magnesium level

    Science.gov (United States)

    Low magnesium level is a condition in which the amount of magnesium in the blood is lower than normal. The medical ... that convert or use energy ( metabolism ). When the level of magnesium in the body drops below normal, ...

  4. Beyond level planarity

    NARCIS (Netherlands)

    Angelini, P.; Da Lozzo, G.; Di Battista, G.; Frati, F.; Patrignani, M.; Rutter, I.; Hu, Y.; Nöllenburg, M.

    2016-01-01

    In this paper we settle the computational complexity of two open problems related to the extension of the notion of level planarity to surfaces different from the plane. Namely, we show that the problems of testing the existence of a level embedding of a level graph on the surface of the rolling

  5. Liquid metal level measurement

    International Nuclear Information System (INIS)

    Hale, J.C.; Leyland, K.S.

    1982-01-01

    A liquid metal level indicator is described which can be used to measure, in a stainless steel tank, the level of a nuclear reactor coolant such as sodium. The instrument, which is based on the eddy current induction effect, gives readings over substantially the full depth of the tank and indicates the sense of change of level. (U.K.)

  6. Precision contact level gauge

    International Nuclear Information System (INIS)

    Krejci, M.; Pilat, M.; Stulik, P.

    1977-01-01

    Equipment was developed measuring the heavy water level in the TR-0 reactor core within an accuracy of several hundredths of a millimeter in a range of around 3.5 m and at a temperature of up to 90 degC. The equipment uses a vibrating needle contact as a high sensitivity level gauge and a servomechanical system with a motion screw carrying the gauge for monitoring and measuring the level in the desired range. The advantage of the unique level gauge consists in that that the transducer converts the measured level position to an electric signal, ie., pulse width, with high sensitivity and without hysteresis. (Kr)

  7. High-level verification

    CERN Document Server

    Lerner, Sorin; Kundu, Sudipta

    2011-01-01

    Given the growing size and heterogeneity of Systems on Chip (SOC), the design process from initial specification to chip fabrication has become increasingly complex. This growing complexity provides incentive for designers to use high-level languages such as C, SystemC, and SystemVerilog for system-level design. While a major goal of these high-level languages is to enable verification at a higher level of abstraction, allowing early exploration of system-level designs, the focus so far for validation purposes has been on traditional testing techniques such as random testing and scenario-based

  8. Multi-Level Model

    Directory of Open Access Journals (Sweden)

    Constanta Nicoleta BODEA

    2008-01-01

    Full Text Available Is an original paper, which contains a hierarchical model with three levels, for determining the linearized non-homogeneous and homogeneous credibility premiums at company level, at sector level and at contract level, founded on the relevant covariance relations between the risk premium, the observations and the weighted averages. We give a rather explicit description of the input data for the multi- level hierarchical model used, only to show that in practical situations, there will always be enough data to apply credibility theory to a real insurance portfolio.

  9. Sea level report

    International Nuclear Information System (INIS)

    Schwartz, M.L.

    1979-01-01

    Study of Cenozoic Era sea levels shows a continual lowering of sea level through the Tertiary Period. This overall drop in sea level accompanied the Pleistocene Epoch glacio-eustatic fluctuations. The considerable change of Pleistocene Epoch sea level is most directly attributable to the glacio-eustatic factor, with a time span of 10 5 years and an amplitude or range of approximately 200 m. The lowering of sea level since the end of the Cretaceous Period is attributed to subsidence and mid-ocean ridges. The maximum rate for sea level change is 4 cm/y. At present, mean sea level is rising at about 3 to 4 mm/y. Glacio-eustacy and tectono-eustacy are the parameters for predicting sea level changes in the next 1 my. Glacio-eustatic sea level changes may be projected on the basis of the Milankovitch Theory. Predictions about tectono-eustatic sea level changes, however, involve predictions about future tectonic activity and are therefore somewhat difficult to make. Coastal erosion and sedimentation are affected by changes in sea level. Erosion rates for soft sediments may be as much as 50 m/y. The maximum sedimentation accumulation rate is 20 m/100 y

  10. System level ESD protection

    CERN Document Server

    Vashchenko, Vladislav

    2014-01-01

    This book addresses key aspects of analog integrated circuits and systems design related to system level electrostatic discharge (ESD) protection.  It is an invaluable reference for anyone developing systems-on-chip (SoC) and systems-on-package (SoP), integrated with system-level ESD protection. The book focuses on both the design of semiconductor integrated circuit (IC) components with embedded, on-chip system level protection and IC-system co-design. The readers will be enabled to bring the system level ESD protection solutions to the level of integrated circuits, thereby reducing or completely eliminating the need for additional, discrete components on the printed circuit board (PCB) and meeting system-level ESD requirements. The authors take a systematic approach, based on IC-system ESD protection co-design. A detailed description of the available IC-level ESD testing methods is provided, together with a discussion of the correlation between IC-level and system-level ESD testing methods. The IC-level ESD...

  11. Gamma spectrometry; level schemes

    International Nuclear Information System (INIS)

    Blachot, J.; Bocquet, J.P.; Monnand, E.; Schussler, F.

    1977-01-01

    The research presented dealt with: a new beta emitter, isomer of 131 Sn; the 136 I levels fed through the radioactive decay of 136 Te (20.9s); the A=145 chain (β decay of Ba, La and Ce, and level schemes for 145 La, 145 Ce, 145 Pr); the A=47 chain (La and Ce, β decay, and the level schemes of 147 Ce and 147 Pr) [fr

  12. Nuclear Level Densities

    International Nuclear Information System (INIS)

    Grimes, S.M.

    2005-01-01

    Recent research in the area of nuclear level densities is reviewed. The current interest in nuclear astrophysics and in structure of nuclei off of the line of stability has led to the development of radioactive beam facilities with larger machines currently being planned. Nuclear level densities for the systems used to produce the radioactive beams influence substantially the production rates of these beams. The modification of level-density parameters near the drip lines would also affect nucleosynthesis rates and abundances

  13. Levels of safety

    International Nuclear Information System (INIS)

    Povyakalo, A.A.

    1996-01-01

    When speaking about danger of catastrophe, it is the first level of danger. Its absence is the first level of safety. When speaking about danger of danger of catastrophe, it is the second level of danger. Its absence is the second level of safety. The paper proposes the way to formalize these ideas and use formal models to construct the states-and-event scale for a given object. The proposed approach can be applied to objects of different nature. The states-and-events scale may be used for transformation of initial objectives state-and-transitions graph to reduce bad classes of states

  14. Tiltmeter leveling mechanism

    Science.gov (United States)

    Hunter, Steven L.; Boro, Carl O.; Farris, Alvis

    2002-01-01

    A tiltmeter device having a pair of orthogonally disposed tilt sensors that are levelable within an inner housing containing the sensors. An outer housing can be rotated to level at least one of the sensor pair while the inner housing can be rotated to level the other sensor of the pair. The sensors are typically rotated up to about plus or minus 100 degrees. The device is effective for measuring tilts in a wide range of angles of inclination of wells and can be employed to level a platform containing a third sensor.

  15. Standard Industry Fare Level

    Data.gov (United States)

    Department of Transportation — Standard Industry Fare Level was establish after airline deregulation to serve as the standard against which a statutory zone of operating expense reasonableness was...

  16. PEP liquid level system

    International Nuclear Information System (INIS)

    Lauritzen, T.; Sah, R.C.

    1981-03-01

    A liquid level system has been installed in the accelerator housing of the PEP storage ring. This instrument spans the entire 2.2 km circumference of the PEP project, and over one hundred readouts provide reference elevations which are used for the accurate alignment of accelerator components. The liquid level has proven to be extremely precise (+-0.10 mm) and quick to use, and it has contributed to the accurate alignment of PEP before beam turn-on. Since the liquid level readouts are rigidly attached to the accelerator housing, the liquid level has been a convenient means to monitor the settling of the accelerator housing

  17. Viability testing of material derived from Mycobacterium tuberculosis prior to removal from a containment level-III laboratory as part of a Laboratory Risk Assessment Program.

    Science.gov (United States)

    Blackwood, Kym S; Burdz, Tamara V; Turenne, Christine Y; Sharma, Meenu K; Kabani, Amin M; Wolfe, Joyce N

    2005-01-24

    In the field of clinical mycobacteriology, Mycobacterium tuberculosis (MTB) can be a difficult organism to manipulate due to the restrictive environment of a containment level 3 (CL3) laboratory. Tests for rapid diagnostic work involving smears and molecular methods do not require CL3 practices after the organism has been rendered non-viable. While it has been assumed that after organism deactivation these techniques can be performed outside of a CL3, no conclusive study has consistently confirmed that the organisms are noninfectious after the theoretical 'deactivation' steps. Previous studies have shown that initial steps (such as heating/chemical fixation) may not consistently kill MTB organisms. An inclusive viability study (n = 226) was undertaken to determine at which point handling of culture extraction materials does not necessitate a CL3 environment. Four different laboratory protocols tested for viability included: standard DNA extractions for IS6110 fingerprinting, crude DNA preparations for PCR by boiling and mechanical lysis, protein extractions, and smear preparations. For each protocol, laboratory staff planted a proportion of the resulting material to Bactec 12B medium that was observed for growth for 8 weeks. Of the 208 isolates initially tested, 21 samples grew within the 8-week period. Sixteen (7.7%) of these yielded positive results for MTB that included samples of: deactivated culture resuspensions exposed to 80 degrees C for 20 minutes, smear preparations and protein extractions. Test procedures were consequently modified and tested again (n = 18), resulting in 0% viability. This study demonstrates that it cannot be assumed that conventional practices (i.e. smear preparation) or extraction techniques render the organism non-viable. All methodologies, new and existing, should be examined by individual laboratories to validate the safe removal of material derived from MTB to the outside of a CL3 laboratory. This process is vital to establish in

  18. Viability testing of material derived from Mycobacterium tuberculosis prior to removal from a Containment Level-III Laboratory as part of a Laboratory Risk Assessment Program

    Directory of Open Access Journals (Sweden)

    Kabani Amin M

    2005-01-01

    Full Text Available Abstract Background In the field of clinical mycobacteriology, Mycobacterium tuberculosis (MTB can be a difficult organism to manipulate due to the restrictive environment of a containment level 3 (CL3 laboratory. Tests for rapid diagnostic work involving smears and molecular methods do not require CL3 practices after the organism has been rendered non-viable. While it has been assumed that after organism deactivation these techniques can be performed outside of a CL3, no conclusive study has consistently confirmed that the organisms are noninfectious after the theoretical 'deactivation' steps. Previous studies have shown that initial steps (such as heating /chemical fixation may not consistently kill MTB organisms. Methods An inclusive viability study (n = 226 was undertaken to determine at which point handling of culture extraction materials does not necessitate a CL3 environment. Four different laboratory protocols tested for viability included: standard DNA extractions for IS6110 fingerprinting, crude DNA preparations for PCR by boiling and mechanical lysis, protein extractions, and smear preparations. For each protocol, laboratory staff planted a proportion of the resulting material to Bactec 12B medium that was observed for growth for 8 weeks. Results Of the 208 isolates initially tested, 21 samples grew within the 8-week period. Sixteen (7.7% of these yielded positive results for MTB that included samples of: deactivated culture resuspensions exposed to 80°C for 20 minutes, smear preparations and protein extractions. Test procedures were consequently modified and tested again (n = 18, resulting in 0% viability. Conclusions This study demonstrates that it cannot be assumed that conventional practices (i.e. smear preparation or extraction techniques render the organism non-viable. All methodologies, new and existing, should be examined by individual laboratories to validate the safe removal of material derived from MTB to the outside of a

  19. Reference handbook: Level detectors

    International Nuclear Information System (INIS)

    1990-01-01

    The purpose of this handbook is to provide Rocky Flats personnel with the information necessary to understand level measurement and detection. Upon completion of this handbook you should be able to do the following: List three reasons for measuring level. Describe the basic operating principles of the sight glass. Demonstrate proper techniques for reading a sight glass. Describe the basic operating principles of a float level detector. Describe the basic operating principles of a bubbler level indicating system. Explain the differences between a wet and dry reference leg indicating system, and describe how each functions. This handbook is designed for use by experienced Rocky Flats operators to reinforce and improve their current knowledge level, and by entry-level operators to ensure that they possess a minimum level of fundamental knowledge. Level Detectors is applicable to many job classifications and can be used as a reference for classroom work or for self-study. Although this reference handbook is by no means all-encompassing, you will gain enough information about this subject area to assist you in contributing to the safe operation of Rocky Flats Plant

  20. Authentication Assurance Levels

    International Nuclear Information System (INIS)

    Kouzes, Richard T.; Cash, James R.; Devaney, David M.; Geelhood, Bruce D.; Hansen, Randy R.; Melton, Ronald B.; Pitts, W. Karl

    2002-01-01

    This Common Criteria approach has been applied to create a definition of Authentication Assurance Levels that can quantify the level of assurance reached for a system subject to a set of authentication procedures. The arms-control authentication application of the Common Criteria expands on more typical information security evaluations in that it must contend with information barriers and preclude sophisticated intentional subversion attempts.

  1. Raised IL-6 Levels

    African Journals Online (AJOL)

    user

    Cardiovascular Associated Complications in HIV. Positive Zambians before ... compare plasma levels of IL-6 in HIV positive and. HIV negative .... cancer. Results from this study showed that IL-6 levels in. HIV positive ART naive individuals were significantly higher than in the HIV positive individuals on ART. Our findings ...

  2. Low level waste repositories

    International Nuclear Information System (INIS)

    Hill, P.R.H.; Wilson, M.A.

    1983-11-01

    Factors in selecting a site for low-level radioactive waste disposal are discussed. South Australia has used a former tailings dam in a remote, arid location as a llw repository. There are also low-level waste disposal procedures at the Olympic Dam copper/uranium project

  3. Definition of Virtual Levels.

    Science.gov (United States)

    Shore, Bruce W.

    1979-01-01

    Presents an examination of graphical displays of solutions to time-dependent Schrodinger equation modeling a laser-excited three-level atom. It suggests that an energy level may be regarded as virtual when it is detuned from resonance by more than two Rabi frequencies. (Author/HM)

  4. Levels of Literary Meaning

    DEFF Research Database (Denmark)

    Klausen, Søren Harnow

    2017-01-01

    I argue that intentionalist theories of meaning and interpretation, like those of Hirsch and Juhl, have been insufficiently attentive to the different levels of authorial intention that are operative in literary works. By countenancing intentions on different levels – ranging from simple semantic...

  5. Arctic Sea Level Reconstruction

    DEFF Research Database (Denmark)

    Svendsen, Peter Limkilde

    Reconstruction of historical Arctic sea level is very difficult due to the limited coverage and quality of tide gauge and altimetry data in the area. This thesis addresses many of these issues, and discusses strategies to help achieve a stable and plausible reconstruction of Arctic sea level from...... 1950 to today.The primary record of historical sea level, on the order of several decades to a few centuries, is tide gauges. Tide gauge records from around the world are collected in the Permanent Service for Mean Sea Level (PSMSL) database, and includes data along the Arctic coasts. A reasonable...... amount of data is available along the Norwegian and Russian coasts since 1950, and most published research on Arctic sea level extends cautiously from these areas. Very little tide gauge data is available elsewhere in the Arctic, and records of a length of several decades,as generally recommended for sea...

  6. Contemporary Arctic Sea Level

    Science.gov (United States)

    Cazenave, A. A.

    2017-12-01

    During recent decades, the Arctic region has warmed at a rate about twice the rest of the globe. Sea ice melting is increasing and the Greenland ice sheet is losing mass at an accelerated rate. Arctic warming, decrease in the sea ice cover and fresh water input to the Arctic ocean may eventually impact the Arctic sea level. In this presentation, we review our current knowledge of contemporary Arctic sea level changes. Until the beginning of the 1990s, Arctic sea level variations were essentially deduced from tide gauges located along the Russian and Norwegian coastlines. Since then, high inclination satellite altimetry missions have allowed measuring sea level over a large portion of the Arctic Ocean (up to 80 degree north). Measuring sea level in the Arctic by satellite altimetry is challenging because the presence of sea ice cover limits the full capacity of this technique. However adapted processing of raw altimetric measurements significantly increases the number of valid data, hence the data coverage, from which regional sea level variations can be extracted. Over the altimetry era, positive trend patterns are observed over the Beaufort Gyre and along the east coast of Greenland, while negative trends are reported along the Siberian shelf. On average over the Arctic region covered by satellite altimetry, the rate of sea level rise since 1992 is slightly less than the global mea sea level rate (of about 3 mm per year). On the other hand, the interannual variability is quite significant. Space gravimetry data from the GRACE mission and ocean reanalyses provide information on the mass and steric contributions to sea level, hence on the sea level budget. Budget studies show that regional sea level trends over the Beaufort Gyre and along the eastern coast of Greenland, are essentially due to salinity changes. However, in terms of regional average, the net steric component contributes little to the observed sea level trend. The sea level budget in the Arctic

  7. Nuclear level density

    International Nuclear Information System (INIS)

    Cardoso Junior, J.L.

    1982-10-01

    Experimental data show that the number of nuclear states increases rapidly with increasing excitation energy. The properties of highly excited nuclei are important for many nuclear reactions, mainly those that go via processes of the compound nucleus type. In this case, it is sufficient to know the statistical properties of the nuclear levels. First of them is the function of nuclear levels density. Several theoretical models which describe the level density are presented. The statistical mechanics and a quantum mechanics formalisms as well as semi-empirical results are analysed and discussed. (Author) [pt

  8. Measuring Your Fitness Level

    Science.gov (United States)

    ... online calculator. If you'd rather do the math yourself, divide your weight in pounds by your ... Human Services recommends one of the following activity levels for adult fitness and health benefits: 150 minutes ...

  9. Three Levels of Semiosis

    DEFF Research Database (Denmark)

    Alrøe, Hugo Fjelsted

    2016-01-01

    This paper presents a second-order semiotic framework for ontological levels, which is based on the structure of semiosis in a more fundamental way than other existing approaches to semiotic levels. Building on the key semiotic concept of representation, three levels are posited: (1) non- or proto......-semiotic processes, without representation, such as physical or causal processes, (2) semiotic processes, with representation, such as the processes of life and cognition, and (3) second-order semiotic processes, with representation of representation, such as self-conscious and self-reflexive communicative processes....... This semiotic framework for ontological levels is compared with recent discussions of different ontological kinds of kinds such as indifferent and interactive kinds. This leads to the distinction between not two, but three kinds of kinds: indifferent, adaptive and reflexive kinds, of which the last two hitherto...

  10. Water Level Station History

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Images contain station history information for 175 stations in the National Water Level Observation Network (NWLON). The NWLON is a network of long-term,...

  11. Projecting future sea level

    Science.gov (United States)

    Cayan, Daniel R.; Bromirski, Peter; Hayhoe, Katharine; Tyree, Mary; Dettinger, Mike; Flick, Reinhard

    2006-01-01

    California’s coastal observations and global model projections indicate that California’s open coast and estuaries will experience increasing sea levels over the next century. Sea level rise has affected much of the coast of California, including the Southern California coast, the Central California open coast, and the San Francisco Bay and upper estuary. These trends, quantified from a small set of California tide gages, have ranged from 10–20 centimeters (cm) (3.9–7.9 inches) per century, quite similar to that estimated for global mean sea level. So far, there is little evidence that the rate of rise has accelerated, and the rate of rise at California tide gages has actually flattened since 1980, but projections suggest substantial sea level rise may occur over the next century. Climate change simulations project a substantial rate of global sea level rise over the next century due to thermal expansion as the oceans warm and runoff from melting land-based snow and ice accelerates. Sea level rise projected from the models increases with the amount of warming. Relative to sea levels in 2000, by the 2070–2099 period, sea level rise projections range from 11–54 cm (4.3–21 in) for simulations following the lower (B1) greenhouse gas (GHG) emissions scenario, from 14–61 cm (5.5–24 in) for the middle-upper (A2) emission scenario, and from 17–72 cm (6.7–28 in) for the highest (A1fi) scenario. In addition to relatively steady secular trends, sea levels along the California coast undergo shorter period variability above or below predicted tide levels and changes associated with long-term trends. These variations are caused by weather events and by seasonal to decadal climate fluctuations over the Pacific Ocean that in turn affect the Pacific coast. Highest coastal sea levels have occurred when winter storms and Pacific climate disturbances, such as El Niño, have coincided with high astronomical tides. This study considers a range of projected future

  12. High potassium level

    Science.gov (United States)

    ... level is very high, or if you have danger signs, such as changes in an ECG . Emergency ... Seifter JL. Potassium disorders. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine . 25th ed. Philadelphia, PA: ...

  13. Technology Readiness Level Guidebook

    Science.gov (United States)

    2017-09-01

    This guidebook provides the necessary information for conducting a Technology Readiness Level (TRL) Assessment. TRL Assessments are a tool for determining the maturity of technologies and identifying next steps in the research process. This guidebook...

  14. Low potassium level

    Science.gov (United States)

    ... treat and prevent low level of potassium. These foods include: Avocados Baked potato Bananas Bran Carrots Cooked lean beef Milk Oranges Peanut butter Peas and beans Salmon Seaweed Spinach Tomatoes Wheat germ

  15. Low level waste disposal

    International Nuclear Information System (INIS)

    Barthoux, A.

    1985-01-01

    Final disposal of low level wastes has been carried out for 15 years on the shallow land disposal of the Manche in the north west of France. Final participant in the nuclear energy cycle, ANDRA has set up a new waste management system from the production center (organization of the waste collection) to the disposal site including the setting up of a transport network, the development of assessment, additional conditioning, interim storage, the management of the disposal center, records of the location and characteristics of the disposed wastes, site selection surveys for future disposals and a public information Department. 80 000 waste packages representing a volume of 20 000 m 3 are thus managed and disposed of each year on the shallow land disposal. The disposal of low level wastes is carried out according to their category and activity level: - in tumuli for very low level wastes, - in monoliths, a concrete structure, of the packaging does not provide enough protection against radioactivity [fr

  16. Lead levels - blood

    Science.gov (United States)

    Blood lead levels ... is used to screen people at risk for lead poisoning. This may include industrial workers and children ... also used to measure how well treatment for lead poisoning is working. Lead is common in the ...

  17. Lake Level Reconstructions

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Records of past lake levels, mostly related to changes in moisture balance (evaporation-precipitation). Parameter keywords describe what was measured in this data...

  18. Liquid level controller

    International Nuclear Information System (INIS)

    Mangus, J.D.; Redding, A.H.

    1975-01-01

    A system for maintaining two distinct sodium levels within the shell of a heat exchanger having a plurality of J-shaped modular tube bundles each enclosed in a separate shell which extends from a common base portion. A lower liquid level is maintained in the base portion and an upper liquid level is maintained in the shell enwrapping the long stem of the J-shaped tube bundles by utilizing standpipes with a notch at the lower end which decreases in open area the distance from the end of the stand pipe increases and a supply of inert gas fed at a constant rate to produce liquid levels, which will remain generally constant as the flow of liquid through the vessel varies

  19. Fission level densities

    International Nuclear Information System (INIS)

    Maslov, V.M.

    1998-01-01

    Fission level densities (or fissioning nucleus level densities at fission saddle deformations) are required for statistical model calculations of actinide fission cross sections. Back-shifted Fermi-Gas Model, Constant Temperature Model and Generalized Superfluid Model (GSM) are widely used for the description of level densities at stable deformations. These models provide approximately identical level density description at excitations close to the neutron binding energy. It is at low excitation energies that they are discrepant, while this energy region is crucial for fission cross section calculations. A drawback of back-shifted Fermi gas model and traditional constant temperature model approaches is that it is difficult to include in a consistent way pair correlations, collective effects and shell effects. Pair, shell and collective properties of nucleus do not reduce just to the renormalization of level density parameter a, but influence the energy dependence of level densities. These effects turn out to be important because they seem to depend upon deformation of either equilibrium or saddle-point. These effects are easily introduced within GSM approach. Fission barriers are another key ingredients involved in the fission cross section calculations. Fission level density and barrier parameters are strongly interdependent. This is the reason for including fission barrier parameters along with the fission level densities in the Starter File. The recommended file is maslov.dat - fission barrier parameters. Recent version of actinide fission barrier data obtained in Obninsk (obninsk.dat) should only be considered as a guide for selection of initial parameters. These data are included in the Starter File, together with the fission barrier parameters recommended by CNDC (beijing.dat), for completeness. (author)

  20. Changing Sea Levels

    Science.gov (United States)

    Pugh, David

    2004-04-01

    Flooding of coastal communities is one of the major causes of environmental disasters world-wide. This textbook explains how sea levels are affected by astronomical tides, weather effects, ocean circulation and climate trends. Based on courses taught by the author in the U.K. and the U.S., it is aimed at undergraduate students at all levels, with non-basic mathematics being confined to Appendices and a website http://publishing.cambridge.org/resources/0521532183/.

  1. Float level indicator

    International Nuclear Information System (INIS)

    Grishchuk, M.Kh.; Laptev, A.G.; Pashkov, V.A.

    1980-01-01

    Specially developed level indicator is suggested with differential to-transformer converter of the float motion, operating in line with a movable electronic block, intended for indicating the level of the dissociating nitrogen tetroxide liquid phase. On the basis of the indicator elements the device is realized to measure the time of calibrated volume fillino. in by liquid nitrogen tetroxide in steady state operation of the experimental bench-marks [ru

  2. Contemporary sea level rise.

    Science.gov (United States)

    Cazenave, Anny; Llovel, William

    2010-01-01

    Measuring sea level change and understanding its causes has considerably improved in the recent years, essentially because new in situ and remote sensing observations have become available. Here we report on most recent results on contemporary sea level rise. We first present sea level observations from tide gauges over the twentieth century and from satellite altimetry since the early 1990s. We next discuss the most recent progress made in quantifying the processes causing sea level change on timescales ranging from years to decades, i.e., thermal expansion of the oceans, land ice mass loss, and land water-storage change. We show that for the 1993-2007 time span, the sum of climate-related contributions (2.85 +/- 0.35 mm year(-1)) is only slightly less than altimetry-based sea level rise (3.3 +/- 0.4 mm year(-1)): approximately 30% of the observed rate of rise is due to ocean thermal expansion and approximately 55% results from land ice melt. Recent acceleration in glacier melting and ice mass loss from the ice sheets increases the latter contribution up to 80% for the past five years. We also review the main causes of regional variability in sea level trends: The dominant contribution results from nonuniform changes in ocean thermal expansion.

  3. Water level indicator

    International Nuclear Information System (INIS)

    Murase, Michio; Araki, Hidefumi.

    1996-01-01

    A difference of pressure between a standard pressure conduit in communication with a gas phase of a reactor pressure vessel and a water level pressure conduit in communication with a liquid phase of the pressure vessel is detected by a pressure difference gage. A communication pipe and a standard level vessel are disposed between the pressure vessel and the standard pressure conduit, and a standard liquid surface on the side of the standard pressure conduit is formed in the standard level vessel. A gas releaser is disposed to the gas phase portion of the standard level vessel. The gas releaser equipment is constituted by a porous material, a permeation membrane and a gas exhaustion hole. The gas phase of the standard level vessel is divided by a partition plate into a first gas phase being in contact with a connection portion with the communication pipe and a second gas phase in contact with the gas releaser. A gas flow channel hole and a condensate descending hole are disposed to the partition plate. Since incondensible gases accumulated to the standard level vessel are effectively exhausted, the incondensible gases are prevented from being dissolved into liquid. (I.N.)

  4. U.S. Army Medical Research Institute of Infectious Diseases

    Science.gov (United States)

    ... members from biological threats. Our specialized capabilities include Biosafety Level 3 and Level 4 laboratories, world-class ... for the Department of Defense, we set the standard for identification of biological agents. Our customers in ...

  5. Transmutations across hierarchical levels

    International Nuclear Information System (INIS)

    O'Neill, R.V.

    1977-01-01

    The development of large-scale ecological models depends implicitly on a concept known as hierarchy theory which views biological systems in a series of hierarchical levels (i.e., organism, population, trophic level, ecosystem). The theory states that an explanation of a biological phenomenon is provided when it is shown to be the consequence of the activities of the system's components, which are themselves systems in the next lower level of the hierarchy. Thus, the behavior of a population is explained by the behavior of the organisms in the population. The initial step in any modeling project is, therefore, to identify the system components and the interactions between them. A series of examples of transmutations in aquatic and terrestrial ecosystems are presented to show how and why changes occur. The types of changes are summarized and possible implications of transmutation for hierarchy theory, for the modeler, and for the ecological theoretician are discussed

  6. Optimal Levels of Embeddedness

    DEFF Research Database (Denmark)

    Andersen, Kristina Vaarst

    Co-location of industry professionals often leads to development of collaboration networks, and multiple studies have emphasized the benefits of embedded collaboration. Due to higher levels of trust, embedded collaboration reduces transaction costs and facilitates ready knowledge exchanged. Other...... on regional level data on co-location of knowledge workers, workplaces, and cultural amenities. Two papers build on individual level data from the Danish film industry. The first paper analyses why the importance of co-location differs between groups of knowledge workers and aim to explain centralization...... in localized collaboration networks is so essential, it affects location choices. The second paper challenges the proposition of embeddedness as an absolute term through an analysis of costs and benefits of embeddedness in an industry network. The results show that the association between embeddedness...

  7. Caribbean Sea Level Network

    Science.gov (United States)

    von Hillebrandt-Andrade, C.; Crespo Jones, H.

    2012-12-01

    Over the past 500 years almost 100 tsunamis have been observed in the Caribbean and Western Atlantic, with at least 3510 people having lost their lives to this hazard since 1842. Furthermore, with the dramatic increase in population and infrastructure along the Caribbean coasts, today, millions of coastal residents, workers and visitors are vulnerable to tsunamis. The UNESCO IOC Intergovernmental Coordination Group for Tsunamis and other Coastal Hazards for the Caribbean and Adjacent Regions (CARIBE EWS) was established in 2005 to coordinate and advance the regional tsunami warning system. The CARIBE EWS focuses on four areas/working groups: (1) Monitoring and Warning, (2) Hazard and Risk Assessment, (3) Communication and (4) Education, Preparedness and Readiness. The sea level monitoring component is under Working Group 1. Although in the current system, it's the seismic data and information that generate the initial tsunami bulletins, it is the data from deep ocean buoys (DARTS) and the coastal sea level gauges that are critical for the actual detection and forecasting of tsunamis impact. Despite multiple efforts and investments in the installation of sea level stations in the region, in 2004 there were only a handful of sea level stations operational in the region (Puerto Rico, US Virgin Islands, Bermuda, Bahamas). Over the past 5 years there has been a steady increase in the number of stations operating in the Caribbean region. As of mid 2012 there were 7 DARTS and 37 coastal gauges with additional ones being installed or funded. In order to reach the goal of 100 operational coastal sea level stations in the Caribbean, the CARIBE EWS recognizes also the importance of maintaining the current stations. For this, a trained workforce in the region for the installation, operation and data analysis and quality control is considered to be critical. Since 2008, three training courses have been offered to the sea level station operators and data analysts. Other

  8. Seismic Level 2 PSA

    International Nuclear Information System (INIS)

    Dirksen, Gerben; Pellissetti, Manuel; Duncan-Whiteman, Paul

    2014-01-01

    For most external events, the calculation of the core damage frequency (CDF) in Level 1 PSA is sufficient to be able to show that the contribution of the event to the plant risk is negligible. However, it is not sufficient to compare the CDF due to the external event to the total plant CDF; instead the Level 1 PSA result for the event should be compared to the large early release frequency (LERF), or alternatively arguments should be given why the CDF from the external event will not contribute mostly to LERF. For seismic events in particular, it can often not be easily excluded that sequences leading to core damage would not also result in LERF. Since the confinement function is one of the most essential functions for Level 2 PSA, special care must be taken of the containment penetrations. For example systems with containment penetrations that are normally closed during operation or are designed to withstand more than the maximum containment pressure are normally screened out in the Level 2 PSA for the containment isolation function, however the possibility of LOCA in such systems due to an earthquake may nevertheless lead to containment bypass. Additionally, the functionality of passive features may be compromised in case of a beyond design earthquake. In the present paper, we present crucial ingredients of a methodology for a Level 2 seismic PSA. This methodology consists of the following steps: Extension of the seismic equipment list (SEL) to include Level 2 PSA relevant systems (e.g. containment isolation system, features for core melt stabilization, hydrogen mitigation systems), Determination of the systems within the existing SEL with increased demands in case of severe accidents, Determination of essential components for which a dedicated fragility analysis needs to be performed. (author)

  9. Street level society

    DEFF Research Database (Denmark)

    Vinum, Christine; Nissen, Morten

    2006-01-01

    This paper aims to reflect on research findings from different empirical studies of social work with young drug users and socially excluded young people in Copenhagen. In the paper we account for historical changes in social policy and interventions into young people's drug taking in Copenhagen......, and partly from the decentralizing and specializing efforts characteristic of the Danish welfare state and its institutions. We discuss a general turn towards street level interventions to address the problems of social exclusion, as well as different attempts to create what we term street level heterotopias...

  10. Level width broadening effect

    International Nuclear Information System (INIS)

    Zhang Jingshang

    2003-01-01

    In file-6 for double-differential cross sections, the level width broadening effect should be taken into account properly due to Heisenberg' uncertainty. Besides level width broadening effect, the energy resolution in the measurements is also needed in fitting measurement procedure. In general, the traditional normal Gaussian expansion is employed. However, to do so in this way the energy balance could not be held. For this reason, the deformed Gaussian expansion functions with exponential form for both the single energy point and continuous spectrum are introduced, with which the normalization and energy balance conditions could be held exactly in the analytical form. (author)

  11. Middle Level Learning.

    Science.gov (United States)

    Wyman, Richard M., Jr.; Young, Katherine A.; Sliger, Bruce; Kafi, Patricia; Singer, Alan; Lamme, Linda Leonard

    1998-01-01

    Presents five brief articles related to middle-level learning. The articles are, "Using Children's Diaries to Teach the Oregon Trail"; "Living the Geography of Joseph and Temperance Brown"; "The ABCs of Small Grant Acquisition for Social Studies"; "Isomo Loruko: The Yoruba Naming Ceremony"; and "Child…

  12. New level of structure

    International Nuclear Information System (INIS)

    Greenberg, O.W.

    1985-01-01

    In the standard model of matter, ther are five stages of compositeness- molecules, atoms, nuclei, nucleons, and quarks and leptons-but we are beginning to see regularities at the fifth layer that may point to a deeper, sixth level of structure

  13. Reliability and code level

    NARCIS (Netherlands)

    Kasperski, M.; Geurts, C.P.W.

    2005-01-01

    The paper describes the work of the IAWE Working Group WBG - Reliability and Code Level, one of the International Codification Working Groups set up at ICWE10 in Copenhagen. The following topics are covered: sources of uncertainties in the design wind load, appropriate design target values for the

  14. Organotin levels in seafood

    NARCIS (Netherlands)

    Belfroid, A.C.; Purperhart, M.; Ariese, F.

    2000-01-01

    Tolerable average residue levels (TARL) for tributylin (TBT) in seafood products were calculated based on the tolerable daily intake (TDI) of TBT and the seafood consumption of the average consumer in various countries. Data from the literature show that these TARLs in seafood are exceeded in one or

  15. 55Co level properties

    International Nuclear Information System (INIS)

    Lodin, G.; Nilsson, L.; Erlandsson, B.; Lyttkens, J.

    1975-01-01

    The decay pf low-lying levels in 55 Co has been studied by means of the 54 Fe(d,nγ) 55 Co reaction. Neutron energies were determined by time-of-flight techniques using a pulsed incident beam and a large liquid scintillator. Gamma rays in coincidence with neutrons were detected by a Ge(Li)spectrometer, Three levels at 4720.8+-0.6, 4747.1+-0.6 and 5172.4+-0.6 keV were strongly populated. The 4720 and 5172 keV levels have earlier been identified as the isobaric analogues of the 55 Fe ground state and first excited state. On the basis of a comparison of the present results with previous particle-transfer reaction studies it is suggested that the ground state analogue is split into the 4720 and 4747 keV levels. A study of excitation functions of 54 Fe(d,n)transitions at incident deuteron energies between 5.0 and 6.0 MeV show that the spectroscopic strengths obtained from (d,n) experiments in this mass region at these energies depend strongly on the incident energy. (Auth.)

  16. Implementing Modular A Levels.

    Science.gov (United States)

    Holding, Gordon

    This document, which is designed for curriculum managers at British further education (FE) colleges, presents basic information on the implementation and perceived benefits of the General Certificate of Education (GCE) modular A (Advanced) levels. The information was synthesized from a survey of 12 FE colleges that introduced the modular A levels…

  17. Levels of valence

    Directory of Open Access Journals (Sweden)

    Vera eShuman

    2013-05-01

    Full Text Available The distinction between the positive and the negative is fundamental in our emotional life. In appraisal theories, in particular in the component process model of emotion (Scherer, 1984, 2010, qualitatively different types of valence are proposed based on appraisals of (unpleasantness, goal obstructiveness/conduciveness, low or high power, self- (incongruence, and moral badness/goodness. This multifaceted conceptualization of valence is highly compatible with the frequent observation of mixed feelings in real life. However, it seems to contradict the one-dimensional conceptualization of valence often encountered in psychological theories, and the notion of valence as a common currency used to explain choice behavior. Here, we propose a framework to integrate the seemingly disparate conceptualizations of multifaceted valence and one-dimensional valence by suggesting that valence should be conceived at different levels, micro and macro. Micro-valences correspond to qualitatively different types of evaluations, potentially resulting in mixed feelings, whereas one-dimensional macro-valence corresponds to an integrative common currency to compare alternatives for choices. We propose that conceptualizing levels of valence may focus research attention on the mechanisms that relate valence at one level (micro to valence at another level (macro, leading to new hypotheses and addressing various concerns that have been raised about the valence concept, such as the valence-emotion relation.

  18. Levels of Valence

    Science.gov (United States)

    Shuman, Vera; Sander, David; Scherer, Klaus R.

    2013-01-01

    The distinction between the positive and the negative is fundamental in our emotional life. In appraisal theories, in particular in the component process model of emotion (Scherer, 1984, 2010), qualitatively different types of valence are proposed based on appraisals of (un)pleasantness, goal obstructiveness/conduciveness, low or high power, self-(in)congruence, and moral badness/goodness. This multifaceted conceptualization of valence is highly compatible with the frequent observation of mixed feelings in real life. However, it seems to contradict the one-dimensional conceptualization of valence often encountered in psychological theories, and the notion of valence as a common currency used to explain choice behavior. Here, we propose a framework to integrate the seemingly disparate conceptualizations of multifaceted valence and one-dimensional valence by suggesting that valence should be conceived at different levels, micro and macro. Micro-valences correspond to qualitatively different types of evaluations, potentially resulting in mixed feelings, whereas one-dimensional macro-valence corresponds to an integrative “common currency” to compare alternatives for choices. We propose that conceptualizing levels of valence may focus research attention on the mechanisms that relate valence at one level (micro) to valence at another level (macro), leading to new hypotheses, and addressing various concerns that have been raised about the valence concept, such as the valence-emotion relation. PMID:23717292

  19. Higher-level Innovization

    DEFF Research Database (Denmark)

    Bandaru, Sunith; Tutum, Cem Celal; Deb, Kalyanmoy

    2011-01-01

    we introduce the higher-level innovization task through an application of a manufacturing process simulation for the Friction Stir Welding (FSW) process where commonalities among two different Pareto-optimal fronts are analyzed. Multiple design rules are simultaneously deciphered from each front...

  20. Guideline level-3 PSA

    International Nuclear Information System (INIS)

    Roelofsen, P.M.; Van der Steen, J.

    1993-09-01

    For several applications of radioactive materials calculations must be executed to determine the radiation risk for the population. A guideline for the risk calculation method of two main sources: nuclear power plants, and other intended and unintended activities with radioactive materials, is given. The standards, recommendations and regulations in this report concern mainly the analysis of the radiological (external) consequences of nuclear power plant accidents, classified as level-3 PSA (Probabilistic Safety Analysis). Level-3 PSA falls within the scales 5-7 of the International Nuclear Event Scale (INES). The standards, etc., focus on the risks for groups of people and the so-called maximum individual risk. In chapter two the standards and regulations are formulated for each part of level-3 PSA: the source term spectrum, atmospheric distribution and deposition, exposure to radiation doses and calculation of radiation doses, dose-response relationships, measures to reduce the effect of radiation doses, design basis accidents, and finally uncertainty analysis. In chapter four, modelled descriptions are given of the standards and regulations, which could or should be used in a calculation program in case of level-3 PSA. In chapter three the practical execution of a probabilistic consequences analysis, the collection of input data and the presentation of the results are dealt with. 2 figs., 14 tabs., 64 refs

  1. General Algorithm (High level)

    Indian Academy of Sciences (India)

    First page Back Continue Last page Overview Graphics. General Algorithm (High level). Iteratively. Use Tightness Property to remove points of P1,..,Pi. Use random sampling to get a Random Sample (of enough points) from the next largest cluster, Pi+1. Use the Random Sampling Procedure to approximate ci+1 using the ...

  2. Liquid level detector

    International Nuclear Information System (INIS)

    Dean, A.P.; Pendleton, J.R.

    1982-01-01

    A device is described for locating the level of the liquid metal in a sodium cooled nuclear reactor by using a movable probe equipped to signal a phase change with null on arrival at the interface between two conductively differing media. (U.K.)

  3. Low-level effects

    International Nuclear Information System (INIS)

    Devine, R.T.; Chaput, R.L.

    1987-01-01

    Risk assignments can be made to given practices involving exposure to radiation, because sufficient data are available for the effects of high-dose, low-LET radiation and because sufficient exists in the methods of extrapolation to low doses and low dose rates. The confidence in the extrapolations is based on the fact that the risk is not expected to be overestimated, using the assumptions made (as opposed to the possibility that the extrapolations represent an accurate estimate of the risk). These risk estimates have been applied to the selection of permissible exposure levels, to show that various amounts of radiation involve no greater risk to the worker than the risk expected in another industry that is generally considered safe. The setting of standards for protection from exposure to low levels of ionizing radiation is made by expert committees at the national and international levels who weigh social factors as well as scientific factors. Data on low-level effects may be applied when assigning a ''probability of causation'' to a certain exposure of radiation. This has become a prominent method for arriving at an equitable award for damages caused by such exposure. The generation of these tables requires as many (if not more) social and political considerations as does the setting up of protection criteria. It is impossible to extract a purely scientific conclusion solely from the protection standards and other legal decisions. Sufficient information exists on low-LET radiation that safety standards for exposure can be rationally (if not scientifically) agreed upon

  4. Low-level radiation

    International Nuclear Information System (INIS)

    Myers, D.K.

    1982-05-01

    It is known that the normal incidence of cancer in human populations is increased by exposure to moderately high doses of ionizing radiation. At background radiation levels or at radiation levels which are 100 times greater, the potential health risks are considered to be directly proportional to the total accumulated dose of radiation. Some of the uncertainties associated with this assumption and with the accepted risk estimates have been critically reviewed in this document. The general scientific consensus at present suggests that the accepted risk estimates may exaggerate the actual risk of low levels of sparsely ionizing radiations (beta-, gamma- or X-rays) somewhat but are unlikely to overestimate the actual risks of densely ionizing radiations (fast neutrons, alpha-particles). At the maximum permissible levels of exposure for radiation workers in nuclear power stations, the potential health hazards in terms of life expectancy would be comparable to those encountered in transportation and public utilities or in the construction industry. At the average radiation exposures received by these workers in practice, the potential health hazards are similar to those associated with safe categories of industries. Uranium mining remains a relativly hazardous occupation. In terms of absolute numbers, the genetic hazards, which are less well established, are thought to be smaller than the carcinogenic hazards of radiation when only the first generation is considered but to be of the same order of magnitude as the carcinogenic hazards when the total number of induced genetic disorders is summed over all generations

  5. Levels of Literacy.

    Science.gov (United States)

    Nettle, Keith

    Democracy in western countries now depends on literacy at every level: censuses by which governments can plan for the future; elections which are the cornerstone of democratic choice; local meetings which have agendas and minutes--the whole apparatus of social living is organized and recorded through literacy. This paper is concerned with how…

  6. Identification of platform levels

    DEFF Research Database (Denmark)

    Mortensen, Niels Henrik

    2005-01-01

    reduction, ability to launch a wider product portfolio without increasing resources and reduction of complexity within the whole company. To support the multiple product development process, platform based product development has in many companies such as Philips, VW, Ford etc. proven to be a very effective...... product development in one step and therefore the objective of this paper is to identify levels of platform based product development. The structure of this paper is as follows. First the applied terminology for platforms will be briefly explained and then characteristics between single and multi product...... development will be examined. Based on the identification of the above characteristics five platform levels are described. The research presented in this paper is a result of MSc, Ph.D projects at the Technical University of Denmark and consultancy projects within the organisation of Institute of Product...

  7. Tree-level formalism

    International Nuclear Information System (INIS)

    Brandhuber, Andreas; Spence, Bill; Travaglini, Gabriele

    2011-01-01

    We review two novel techniques used to calculate tree-level scattering amplitudes efficiently: MHV diagrams, and on-shell recursion relations. For the MHV diagrams, we consider applications to tree-level amplitudes and focus in particular on the N=4 supersymmetric formulation. We also briefly describe the derivation of loop amplitudes using MHV diagrams. For the recursion relations, after presenting their general proof, we discuss several applications to massless theories with and without supersymmetry, to theories with massive particles, and to graviton amplitudes in general relativity. This article is an invited review for a special issue of Journal of Physics A: Mathematical and Theoretical devoted to 'Scattering amplitudes in gauge theories'. (review)

  8. High level nuclear wastes

    International Nuclear Information System (INIS)

    Lopez Perez, B.

    1987-01-01

    The transformations involved in the nuclear fuels during the burn-up at the power nuclear reactors for burn-up levels of 33.000 MWd/th are considered. Graphs and data on the radioactivity variation with the cooling time and heat power of the irradiated fuel are presented. Likewise, the cycle of the fuel in light water reactors is presented and the alternatives for the nuclear waste management are discussed. A brief description of the management of the spent fuel as a high level nuclear waste is shown, explaining the reprocessing and giving data about the fission products and their radioactivities, which must be considered on the vitrification processes. On the final storage of the nuclear waste into depth geological burials, both alternatives are coincident. The countries supporting the reprocessing are indicated and the Spanish programm defined in the Plan Energetico Nacional (PEN) is shortly reviewed. (author) 8 figs., 4 tabs

  9. Acceptable noise level

    DEFF Research Database (Denmark)

    Olsen, Steen Østergaard; Nielsen, Lars Holme; Lantz, Johannes

    2012-01-01

    The acceptable noise level (ANL) is used to quantify the amount of background noise that subjects can accept while listening to speech, and is suggested for prediction of individual hearing-aid use. The aim of this study was to assess the repeatability of the ANL measured in normal-hearing subjects...... using running Danish and non-semantic speech materials as stimuli and modulated speech-spectrum and multi-talker babble noises as competing stimuli....

  10. Service Level Status

    CERN Multimedia

    Lopienski, S

    2007-01-01

    Nowadays, IT departments provide, and people use, computing services of an increasingly heterogeneous nature. There is thus a growing need for a status display that groups these different services and reports status and availability in a uniform way. The Service Level Status (SLS) system addresses these needs by providing a web-based display that dynamically shows availability, basic information and statistics about various IT services, as well as the dependencies between them.

  11. Liquid Level Sensing System

    Science.gov (United States)

    Korman, Valentin (Inventor); Wiley, John T. (Inventor); Duffell, Amanda G. (Inventor)

    2014-01-01

    A liquid level sensing system includes waveguides disposed in a liquid and distributed along a path with a gap between adjacent waveguides. A source introduces electromagnetic energy into the waveguides at a first end of the path. A portion of the electromagnetic energy exits the waveguides at a second end of the path. A detector measures the portion of the electromagnetic energy exiting the second end of the path.

  12. Level up Book Club

    Science.gov (United States)

    LaGarde, Jennifer; Winner, Matthew C.

    2012-01-01

    Like all great ideas, Level Up Book Club grew out of a genuine need, the spontaneous firing of a few brain sparks, and the kind of luck that comes from being "in the right place at the right time." By mid-June 2011 the authors were already "bona fide" wonder twins--two educators who, although they'd never met, had stumbled upon each other through…

  13. Current level detector

    International Nuclear Information System (INIS)

    Kerns, C.R.

    1977-01-01

    A device is provided for detecting the current level of a dc signal. It includes an even harmonic modulator to which a reference ac signal is applied. The unknown dc signal acts on the reference ac signal so that the output of the modulator includes an even harmonic whose amplitude is proportional to the unknown dc current. The device may be used to provide overcurrent protection for proportional wire chambers

  14. Acceptable noise level

    DEFF Research Database (Denmark)

    Olsen, Steen Østergaard; Nielsen, Lars Holme; Lantz, Johannes

    2012-01-01

    The acceptable noise level (ANL) is used to quantify the amount of background noise that subjects can accept while listening to speech, and is suggested for prediction of individual hearing-aid use. The aim of this study was to assess the repeatability of the ANL measured in normal-hearing subjec...... using running Danish and non-semantic speech materials as stimuli and modulated speech-spectrum and multi-talker babble noises as competing stimuli....

  15. Multi-level spondylolysis.

    Science.gov (United States)

    Hersh, David S; Kim, Yong H; Razi, Afshin

    2011-01-01

    The incidence of isthmic spondylolysis is approximately 3% to 6% in the general population. Spondylolytic defects involving multiple vertebral levels, on the other hand, are extremely rare. Only a handful of reports have examined the outcomes of surgical treatment of multi-level spondylolysis. Here, we present one case of bilateral pars defects at L3, L4, and L5. The patient, a 46-year-old female, presented with lower back pain radiating into the left lower extremity. Radiographs and CT scans of the lumbar spine revealed bilateral pars defects at L3-L5. The patient underwent lumbar discectomy and interbody fusion of L4-S1 as well as direct repair of the pars defect at L3. There were no postoperative complications, and by seven months the patient had improved clinically. While previous reports describe the use of either direct repair or fusion in the treatment of spondylolysis, we are unaware of reports describing the use of both techniques at adjacent levels.

  16. ENRAF gauge reference level calculations

    Energy Technology Data Exchange (ETDEWEB)

    Huber, J.H., Fluor Daniel Hanford

    1997-02-06

    This document describes the method for calculating reference levels for Enraf Series 854 Level Detectors as installed in the tank farms. The reference level calculation for each installed level gauge is contained herein.

  17. SWITCHED REFERENCE PHASE LOCK LOOP (SRPLL)

    International Nuclear Information System (INIS)

    KERNER, T.

    2001-01-01

    The Brookhaven National Laboratory Relativistic Heavy Ion Collider (RHIC) has two beam synchronous event links (BSL), one for each ring, which use the 28 MHz ring low level rf to distribute event codes synchronously with a precise phase relationship to the beam. During a cogging reset just before injection, the low level rf sine wave is interrupted which causes the BSL receivers to lose lock. Lock loss in turn causes false triggers and other undesirable-effects on the beam position monitors (BPM), ionization profile monitors (IPM), the tune meter and various experiments which use the BSLs. To rectify these problems, a SRPLL has been inserted between the beam synchronous master and the low level rf source. The SRPLL inserts a frequency and phase continuous splice over the dead-band gap in the rf source created during a cogging reset. The splice removes the gap and prevents the distributed BSL receivers from losing lock

  18. Insignificant levels of dose

    International Nuclear Information System (INIS)

    Webb, G.A.M.; McLean, A.S.

    1977-01-01

    The procedures recommended by the International Commission on Radiological Protection (ICRP) for making decisions concerning controllable sources of radiation exposure of the public include 'justification' and 'optimisation'. The tool recommended by the ICRP for reaching these decisions is collective dose or dose commitment supplemented by consideration of doses to individuals. In both these considerations the practical problem arises of whether very small doses to large numbers of people should contribute to the final decision-making process. It may be that at levels of dose which are small increments on natural background, the relationship between dose and effect is linear even though the slope may be close to zero. If so, collective dose is a meaningful concept and the calculation of total detriment for the purpose of justification could legitimately include all doses. In the calculation of collective doses for the purpose of optimisation, which involves decisions on how much money or resource should be allocated to dose reduction, it is necessary to appraise radiation detriment realistically. At low levels of dose to the individual such as those small by comparison with variations in natural background within the UK, the risk to the individual is such that his well-being will not be significantly changed by the presence or absence of the radiation dose. These small doses, which are well below the point at which an individual attaches significance, should not carry a societal significance. Societal acceptance of risk is analysed with a view to assessing a level of possible risk, and hence dose, below which resources should not in general be diverted to secure further reduction. A formulation for collective dose commitment is proposed incorporating a cut-off to exclude insignificant doses. The implications of this formulation in practical situations are discussed

  19. ALICE High Level Trigger

    CERN Multimedia

    Alt, T

    2013-01-01

    The ALICE High Level Trigger (HLT) is a computing farm designed and build for the real-time, online processing of the raw data produced by the ALICE detectors. Events are fully reconstructed from the raw data, analyzed and compressed. The analysis summary together with the compressed data and a trigger decision is sent to the DAQ. In addition the reconstruction of the events allows for on-line monitoring of physical observables and this information is provided to the Data Quality Monitor (DQM). The HLT can process event rates of up to 2 kHz for proton-proton and 200 Hz for Pb-Pb central collisions.

  20. Global physical activity levels

    DEFF Research Database (Denmark)

    Hallal, Pedro C; Andersen, Lars Bo; Bull, Fiona C

    2012-01-01

    To implement effective non-communicable disease prevention programmes, policy makers need data for physical activity levels and trends. In this report, we describe physical activity levels worldwide with data for adults (15 years or older) from 122 countries and for adolescents (13-15-years......-income countries. The proportion of 13-15-year-olds doing fewer than 60 min of physical activity of moderate to vigorous intensity per day is 80·3% (80·1-80·5); boys are more active than are girls. Continued improvement in monitoring of physical activity would help to guide development of policies and programmes......-old) from 105 countries. Worldwide, 31·1% (95% CI 30·9-31·2) of adults are physically inactive, with proportions ranging from 17·0% (16·8-17·2) in southeast Asia to about 43% in the Americas and the eastern Mediterranean. Inactivity rises with age, is higher in women than in men, and is increased in high...

  1. Water level detection pipeline

    International Nuclear Information System (INIS)

    Koshikawa, Yukinobu; Imanishi, Masatoshi; Niizato, Masaru; Takagi, Masahiro

    1998-01-01

    In the present invention, water levels of a feedwater heater and a drain tank in a nuclear power plant are detected at high accuracy. Detection pipeline headers connected to the upper and lower portions of a feedwater heater or a drain tank are connected with each other. The connection line is branched at appropriate two positions and an upper detection pipeline and a lower detection pipeline are connected thereto, and a gauge entrance valve is disposed to each of the detection pipelines. A diaphragm of a pressure difference generator is connected to a flange formed to the end portion. When detecting the change of water level in the feedwater heater or the drain tank as a change of pressure difference, gauge entrance valves on the exit side of the upper and lower detection pipelines are connected by a connection pipe. The gauge entrance valve is closed, a tube is connected to the lower detection pipe to inject water to the diaphragm of the pressure difference generator passing through the connection pipe thereby enabling to calibrate the pressure difference generator. The accuracy of the calibration of instruments is improved and workability thereof upon flange maintenance is also improved. (I.S.)

  2. Structural and Functional Studies on the Fusion and Attachment Envelope Glycoproteins of Nipah Virus and Hendra Virus

    National Research Council Canada - National Science Library

    Bossart, Katharine

    2003-01-01

    Nipah virus (NiV) and Hendra (HeV) virus are emerging, biosafety level 4 paramyxoviruses responsible for fatal zoonotic infections of humans from pigs and horses, respectively, and are the prototypic members of a new Paramyxovirinae...

  3. Lexical Variation and Change in British Sign Language

    Science.gov (United States)

    Stamp, Rose; Schembri, Adam; Fenlon, Jordan; Rentelis, Ramas; Woll, Bencie; Cormier, Kearsy

    2014-01-01

    This paper presents results from a corpus-based study investigating lexical variation in BSL. An earlier study investigating variation in BSL numeral signs found that younger signers were using a decreasing variety of regionally distinct variants, suggesting that levelling may be taking place. Here, we report findings from a larger investigation looking at regional lexical variants for colours, countries, numbers and UK placenames elicited as part of the BSL Corpus Project. Age, school location and language background were significant predictors of lexical variation, with younger signers using a more levelled variety. This change appears to be happening faster in particular sub-groups of the deaf community (e.g., signers from hearing families). Also, we find that for the names of some UK cities, signers from outside the region use a different sign than those who live in the region. PMID:24759673

  4. LHC Report: Level best

    CERN Multimedia

    Mike Lamont for the LHC team

    2012-01-01

    The LHCb experiment is special: there is a limit to the number of the events the detector can handle per bunch crossing. Consequently the maximum luminosity provided in 2012 has been around 4 x1032 cm-2s-1 (compared to the maximum of 7.7 x1033 cm-2s-1 seen by ATLAS and CMS). Nonetheless LHCb still wants to integrate as much luminosity as possible.    To meet LHCb's requirements a luminosity leveling technique is used. A machine setup is chosen that would give a peak luminosity well above the required maximum if the beams are collided head-on at LHCb's interaction point. This peak luminosity is then reduced to the required maximum by moving the two beams transversely apart at the interaction point. As the beam current goes down during a fill, the beams can be moved together in small increments to keep the collision rate constant throughout the fill. In practice, when the LHC goes into collisions in LHCb, the initial luminosity is safely below LHCb's demanded le...

  5. Monitoring Your Blood Sugar Level

    Science.gov (United States)

    ... and NutritionHealth Insurance: Understanding What It CoversHigh Homocysteine Level: How It Affects Your Blood VesselsUnderstanding Your Medical ... Health Resources Healthcare Management Monitoring Your Blood Sugar Level Monitoring Your Blood Sugar Level Share Print What ...

  6. Fasting and nonfasting lipid levels

    DEFF Research Database (Denmark)

    Langsted, Anne; Freiberg, Jacob J; Nordestgaard, Børge G

    2008-01-01

    Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events.......Lipid profiles are usually measured after fasting. We tested the hypotheses that these levels change only minimally in response to normal food intake and that nonfasting levels predict cardiovascular events....

  7. Science Curriculum Guide, Level 4.

    Science.gov (United States)

    Newark School District, DE.

    The fourth of four levels in a K-12 science curriculum is outlined. In Level 4 (grades 9-12), science areas include earth science, biology, chemistry, and physics. Six major themes provide the basis for study in all levels (K-12). These are: Change, Continuity, Diversity, Interaction, Limitation, and Organization. In Level 4, all six themes are…

  8. Emotion-driven level generation

    OpenAIRE

    Togelius, Julian; Yannakakis, Georgios N.

    2016-01-01

    This chapter examines the relationship between emotions and level generation. Grounded in the experience-driven procedural content generation framework we focus on levels and introduce a taxonomy of approaches for emotion-driven level generation. We then review four characteristic level generators of our earlier work that exemplify each one of the approaches introduced. We conclude the chapter with our vision on the future of emotion-driven level generation.

  9. Level density of 57Co

    International Nuclear Information System (INIS)

    Mishra, V.; Boukharouba, N.; Brient, C.E.; Grimes, S.M.; Pedroni, R.S.

    1994-01-01

    Levels in 57 Co have been studied in the region of resolved levels (E 57 Fe(p,n) 57 Co neutron spectrum with resolution ΔE∼5 keV. Seventeen previously unknown levels are located. Level density parameters in the continuum region are deduced from thick target measurements of the same reaction and additional level density information is deduced from Ericson fluctuation studies of the reaction 56 Fe(p,n) 56 Co. A set of level density parameters is found which describes the level density of 57 Co at energies up to 14 MeV. Efforts to obtain level density information from the 56 Fe(d,n) 57 Co reaction were unsuccessful, but estimates of the fraction of the deuteron absorption cross section corresponding to compound nucleus formation are obtained

  10. Reactor water level control device

    International Nuclear Information System (INIS)

    Hiramatsu, Yohei.

    1980-01-01

    Purpose: To increase the rapid response of the waterlevel control converting a reactor water level signal into a non-linear type, when the water level is near to a set value, to stabilize the water level reducting correlatively the reactor water level variation signal to stabilize greatly from the set value, and increasing the variation signal. Constitution: A main vapor flow quality transmitter detects the vapor flow generated in a reactor and introduced into a turbine. A feed water flow transmitter detects the quantity of a feed water flow from the turbine to the reactor, this detected value is sent to an addition operating apparatus. On the other hand, the power signal of the reactor water level transmitter is sent to the addition operating apparatus through a non-linear water level signal converter. The addition operation apparatus generates a signal for requesting the feed water flow quantity from both signals. Upon this occasion, the reactor water level signal converter makes small the reactor water level variation when the reactor level is close the set value, and when the water level deviates greatly from the set value, the reactor water level variation is made large thereby to improve the rapid response of the reactor coater level control. (Yoshino, Y.)

  11. Next Generation Inactivated Polio Vaccine Manufacturing to Support Post Polio-Eradication Biosafety Goals

    NARCIS (Netherlands)

    Thomassen, Y.E.; Oever, van 't A.G.; Oijen, van M.G.C.T.; Wijffels, R.H.; Pol, van der L.A.; Bakker, W.A.M.

    2013-01-01

    Worldwide efforts to eradicate polio caused a tipping point in polio vaccination strategies. A switch from the oral polio vaccine, which can cause circulating and virulent vaccine derived polioviruses, to inactivated polio vaccines (IPV) is scheduled. Moreover, a manufacturing process, using

  12. Assessment of bio-safety of low-cost polyurethane urologic stents used in developing countries.

    Science.gov (United States)

    Roy, Nobhojit; Waingankar, Santosh; Aggarwal, Gaurav

    2012-04-01

    Ureteral stents, despite their ubiquitous use, have not been evaluated for their safety and strength after removal from the patient. While literature is available from the industry with regards to manufacturing and specifications of stents, what happens to a stent after it is inserted into the body, still needs to be explored. We conducted a methodical study of 153 consecutive patients with urological problems who were stented with inexpensive polyurethane stents. Once removed from the patients, the stents were analyzed for breakload, tensile strength, elongation, pH, decomposition temperature, residue as well as diameter change. There was no significant change in the physical and mechanical properties of the stent after clinical use and the variance was within the acceptable range of biomaterials. There was minimal leaching of material and color change in all stents. The cheap polyurethane stents were found to be safe for use in patients, for the short time periods of in situ stenting. The degradation of physical and chemical properties of the stent was not significant. Thus it can be safely said that the stents currently in widespread use are cost-effective and physically safe for short spans of time.

  13. Assessment of bio-safety of low-cost polyurethane urologic stents used in developing countries

    Directory of Open Access Journals (Sweden)

    Nobhojit Roy

    2012-01-01

    Full Text Available Background: Ureteral stents, despite their ubiquitous use, have not been evaluated for their safety and strength after removal from the patient. While literature is available from the industry with regards to manufacturing and specifications of stents, what happens to a stent after it is inserted into the body, still needs to be explored. Materials and Methods: We conducted a methodical study of 153 consecutive patients with urological problems who were stented with inexpensive polyurethane stents. Once removed from the patients, the stents were analyzed for breakload, tensile strength, elongation, pH, decomposition temperature, residue as well as diameter change. Results: There was no significant change in the physical and mechanical properties of the stent after clinical use and the variance was within the acceptable range of biomaterials. There was minimal leaching of material and color change in all stents. Conclusion: The cheap polyurethane stents were found to be safe for use in patients, for the short time periods of in situ stenting. The degradation of physical and chemical properties of the stent was not significant. Thus it can be safely said that the stents currently in widespread use are cost-effective and physically safe for short spans of time.

  14. Narrative Balance Management in an Intelligent Biosafety Training Application for Improving User Performance

    Science.gov (United States)

    Alvarez, Nahum; Sanchez-Ruiz, Antonio; Cavazza, Marc; Shigematsu, Mika; Prendinger, Helmut

    2015-01-01

    The use of three-dimensional virtual environments in training applications supports the simulation of complex scenarios and realistic object behaviour. While these environments have the potential to provide an advanced training experience to students, it is difficult to design and manage a training session in real time due to the number of…

  15. Understanding the metabolic fate and assessing the biosafety of MnO nanoparticles by metabonomic analysis

    International Nuclear Information System (INIS)

    Li, Jinquan; Feng, Jianghua; Chen, Zhong; Zhao, Zhenghuan; Gao, Jinhao

    2013-01-01

    Recently, some types of MnO nanoparticle (Mn-NP) with favorable imaging capacity have been developed to improve the biocompatible profile of the existing Mn-based MRI contrast agent Mn-DPDP; however, the overall bio-effects and potential toxicity remain largely unknown. In this study, 1 H NMR-based metabolic profiling, integrated with traditional biochemical analysis and histopathological examinations, was used to investigate the absorption, distribution, metabolism, excretion and toxicity of Mn-NPs as candidates for MRI contrast agent. The metabolic responses in biofluids (plasma and urine) and tissues (liver, spleen, kidney, lung and brain) from rats could be divided into four classes following Mn-NP administration: Mn biodistribution-dependent, time-dependent, dose-dependent and complicated metabolic variations. The variations of these metabolites involved in lipid, energy, amino acid and other nutrient metabolism, which disclosed the metabolic fate and biological effects of Mn-NPs in rats. The changes of metabolic profile implied that the disturbance and impairment of biological functions induced by Mn-NP exposure were correlated with the particle size and the surface chemistry of nanoparticles. Integration of metabonomic technology with traditional methods provides a promising tool to understand the toxicological behavior of biomedical nanomaterials and will result in informed decision-making during drug development. (paper)

  16. Microbial biosafety of pilot-scale bioreactor treating MTBE and TBA-contaminated drinking water supply.

    Science.gov (United States)

    Schmidt, Radomir; Klemme, David A; Scow, Kate; Hristova, Krassimira

    2012-03-30

    A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigella spp., Campylobacter jejuni, Aeromonas hydrophila, Legionella pneumophila, Vibrio cholerae, Yersinia enterocolytica and Mycobacterium avium in both influent and treated waters from the bioreactor. Total bacteria decreased during FBBR treatment. E. coli, Salmonella and Shigella spp., C. jejuni, V. cholerae, Y. enterocolytica and M. avium were not detected in aquifer water or bioreactor treated water samples. For those pathogens detected, including total coliforms, L. pneumophila and A. hydrophila, numbers were usually lower in treated water than influent samples, suggesting removal during treatment. The detection of particular bacterial species reflected their presence or absence in the influent waters. Copyright © 2012 Elsevier B.V. All rights reserved.

  17. Biosafety of parenteral Brucella abortus RB51 vaccine in bison calves

    Science.gov (United States)

    Roffe, T.J.; Olsen, S.C.; Gidlewski, T.; Jensen, A.E.; Palmer, M.V.; Huber, R.

    1999-01-01

    Vaccination is considered among the primary management tools for reducing brucellosis prevalence in Greater Yellowstone Area (GYA) ungulates. Before their use, however, vaccine safety and efficacy must be demonstrated. Twenty-seven female bison (Bison bison) calves (approx 5 months old) were vaccinated with Brucella abortus Strain RB51 (1.5 x 1010 colony forming units [CFU], subcutaneously) as part of routine management. We assessed the persistence, pathology, shedding, and transmission associated with RB51 by serial necropsy, bacteriology, histopathology, and serology of 20 of these 27 vaccinated calves, and RB51 serology of 10 nonvaccinated, commingling adult females. With the exception of 1 calf, RB51 dot-blot titers at necropsy were <1:80. Strain RB51 was cultured from lymph nodes in 4 of 4 calves at 14 weeks postvaccination (PV), 4 of 4 calves at 18 weeks PV, 1 of 4 calves at 22 weeks PV, 3 of 4 at 26 weeks PV, and 0 of 4 calves at 30 weeks PV. No gross lesions were observed. Mild histologic changes occurred only in a few draining lymph nodes early in sampling. Adverse clinical effects were not observed in vaccinates. Swabs from nasopharynx, conjunctiva, rectum, and vagina were uniformly culture negative for RB51. Strain RB51 dot-blot assays of bison cows were negative at a 1:20 dilution at 26 weeks PV. Our results suggest that RB51 persists longer in bison calves than in domestic cattle and is systemically distributed within lymphatic tissues. However, bison apparently clear the RB51 vaccine strain without shedding, transmission, or significant adverse reactions.

  18. Biosafety research for non-target organism risk assessment of RNAi-based GE plants

    Science.gov (United States)

    Roberts, Andrew F.; Devos, Yann; Lemgo, Godwin N. Y.; Zhou, Xuguo

    2015-01-01

    RNA interference, or RNAi, refers to a set of biological processes that make use of conserved cellular machinery to silence genes. Although there are several variations in the source and mechanism, they are all triggered by double stranded RNA (dsRNA) which is processed by a protein complex into small, single stranded RNA, referred to as small interfering RNAs (siRNA) with complementarity to sequences in genes targeted for silencing. The use of the RNAi mechanism to develop new traits in plants has fueled a discussion about the environmental safety of the technology for these applications, and this was the subject of a symposium session at the 13th ISBGMO in Cape Town, South Africa. This paper continues that discussion by proposing research areas that may be beneficial for future environmental risk assessments of RNAi-based genetically modified plants, with a particular focus on non-target organism assessment. PMID:26594220

  19. Biosafety of spray dried porcine plasma for different viruses of interest for the swine industry

    OpenAIRE

    Pujols i Romeu, Joan

    2015-01-01

    El productes de plasma dessecat per esprai (SDP) tenen un alt contingut proteic i són components útils per a moltes aplicacions, principalment com a productes valuosos per la nutrició animal. El plasma s'obté a partir de sang de porcs sans aptes pel consum humà. Durant l'obtenció, s'afegeixen anticoagulants i la sang s'emmagatzema en tancs refrigerats. Un cop a la planta de processament, la sang es centrifuga, per separar el plasma de la fracció cel∙lular. El plasma es deshidrata en un procés...

  20. Optimization of liquid media and biosafety assessment for algae-lysing bacterium NP23.

    Science.gov (United States)

    Liao, Chunli; Liu, Xiaobo; Shan, Linna

    2014-09-01

    To control algal bloom caused by nutrient pollution, a wild-type algae-lysing bacterium was isolated from the Baiguishan reservoir in Henan province of China and identified as Enterobacter sp. strain NP23. Algal culture medium was optimized by applying a Placket-Burman design to obtain a high cell concentration of NP23. Three minerals (i.e., 0.6% KNO3, 0.001% MnSO4·H2O, and 0.3% K2HPO4) were found to be independent factors critical for obtaining the highest cell concentration of 10(13) CFU/mL, which was 10(4) times that of the control. In the algae-lysing experiment, the strain exhibited a high lysis rate for the 4 algae test species, namely, Chlorella vulgari, Scenedesmus, Microcystis wesenbergii, and Chlorella pyrenoidosa. Acute toxicity and mutagenicity tests showed that the bacterium NP23 had no toxic and mutagenic effects on fish, even in large doses such as 10(7) or 10(9) CFU/mL. Thus, Enterobacter sp. strain NP23 has strong potential application in the microbial algae-lysing project.

  1. Avian Influenza surveillance: on the usability of FTA cards to solve biosafety and transport issues

    NARCIS (Netherlands)

    Kraus, R.H.; Hooft, van W.F.; Waldenstrom, J.; Latorre-Margalef, N.; Ydenberg, R.C.; Prins, H.H.T.

    2011-01-01

    Avian Influenza Viruses (AIVs) infect many mammals, including humans1. These AIVs are diverse in their natural hosts, harboring almost all possible viral subtypes2. Human pandemics of flu originally stem from AIVs3. Many fatal human cases during the H5N1 outbreaks in recent years were reported.

  2. Economics, Ecology and the Development and Use of GMOs: General Considerations and Biosafety Issues

    OpenAIRE

    Tisdell, Clement A.

    2008-01-01

    This article outlines both ecological and socioeconomic issues involved in the development and use of GMOs. It’s argued that while initially new GMOs add to the global stock of biodiversity, in the long-term they result in a decline in biodiversity. Various mechanisms that bring about this decline in genetic diversity are identified. It is suggested that due to evolutionary forces, the desirable genetic properties of some GMO may be eroded in the long-term. They are unlikely to be sustained i...

  3. Biosafety assessment of probiotics used for human consumption: recommendations from the EU-PROSAFE project

    NARCIS (Netherlands)

    Vankerckhoven, V.; Huys, G.; Vancanneyt, M.; Vael, C.; Klare, I.; Romond, M.B.; Entenza, J.M.; Moreillon, P.; Wind, R.D.; Knol, J.; Wiertz, E.; Pot, B.; Vaughan, E.E.; Kahlmeter, G.; Goossens, H.

    2008-01-01

    On June 26-27, 2006, 60 academic and industry scientists gathered during the PROSAFE workshop to discuss recommendations on taxonomy, antibiotic resistance, in vitro assessment of virulence and in vivo assessment of safety of probiotics used for human consumption. For identification of lactic acid

  4. Regulatory, biosafety and safety challenges for novel cells as substrates for human vaccines.

    Science.gov (United States)

    Hess, Ralf D; Weber, Friedemann; Watson, Keith; Schmitt, Siegfried

    2012-04-05

    In the development of novel substrates used for production of human vaccines there has been significant progress made in recent years. Emerging and re-emerging infectious diseases like the recent porcine Influenza A virus (H1N1) pandemic necessitated the availability of unprecedented amounts of vaccines. In addition, the high demand for vaccines in the industrialised countries has also been paralleled by a steep increase in demand in developing countries. The manufacturing capability for viral vaccines produced in embryonated hen eggs and conventional/classical cell substrates, such as chicken embryo fibroblasts, has now reached its capacity limit. This constraint may be overcome by utilising other recognised cell substrates such as Madin Darby Canine Kidney (MDCK) (dog origin), Chinese Hamster Ovary (CHO) (hamster cells) or Vero cells (monkey origin) or as an alternative, introduce new cell substrates of human or avian origin. Using new cell substrates may prove to be a highly replication-proficient way of producing live viral vaccines such as Influenza A viruses. Despite some advantages, cell substrates may pose a small residual risk to humans since some of them are known to be tumourigenic in immunosuppressed animals. However, this residual risk should be considered acceptable by regulators. Safety testing requirements for cell substrates used in the manufacture of vaccines is mandated by published guidance from organisations such as World Health Organization (WHO), United States Food and Drug Administration (FDA), European Medicines Agency (EMA) and International Conferences on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use (ICH) as well as requirements laid down in compendial monographs (Ph. Eur. and USP). This paper considers the guidance contained in these regulatory documents. In addition, the safety challenges and almost arbitrary risk-based classification of cell substrates used in the production of human vaccines together with compliance to GCCP (Good Cell Culture Practice) are discussed. Even though there has been tremendous progress in the last few years, reflected mainly by revisions and updates to regulatory guidance documents, there still is still no consensus between regulators nor significant harmonisation of the guidance documents or monographs. Copyright © 2012 Elsevier Ltd. All rights reserved.

  5. Explosive developments in biotechnology and the role of BTWC in strengthening a global biosecurity - biosafety

    International Nuclear Information System (INIS)

    Bokan, S.

    2009-01-01

    The international community is confronted by a unique challenge in dealing with the threat posed by the potential use of biology and the life sciences in hostile purposes. As we know, the Biological Weapons Convention (BWC) entered into force thirty four years ago. It is a simple instrument, only a few pages long, but its prohibitions are clear, succinct, categorical and definitive, but it is an instrument of principle rather than procedure. Relevant resources of biological and toxin agents, technologies and knowledge are more numerous and more widely distributed than their equivalents in other disarmament fields (chemical, radiological or nuclear). In the 1990s, negotiations were begun on a protocol to strengthen and verify the BWC, which would have added to the Convention the verification elements present in other regimes. After many years of work, this effort collapsed in disagreement and recrimination in 2001. After the Fifth Review Conference in 2002, BWC States Parties succeeded to establish a work program for 2003 to 2005, at which they would work on several specific topics related to better implementation of the Convention. With that approach of the work, the necessary network of collaboration and coordination were developed into a flexible oversight and prevention of the biological and toxin weapons. Experts from all around the world gathered to share experiences and ideas on how to deal with the threat posed by biological weapons. Officials from health, science and agriculture ministries made connections with their counterparts in defense, justice, foreign affairs and security agencies. The explosive developments in biotechnology represent today serious threat and no government or international organization could hope to monitor effectively the tens of thousands of small biotechnology facilities in operation worldwide. Intelligible, this is a problem that needs a collective, multifaceted and multidimensional approach. However, after the Sixth Review Conference States Parties of the BWC formalized the new approach and recognized the importance of safety and security of biological resources and started cooperation with the scientific, medical, commercial and educational communities. So called 2007-2010 BWC inter sessional process of the work started with significant involvement of NGOs, the scientific community, academics, commercial industry and civil society. New synergy among key international organizations as WHO, OIE, FAO and OPCW, and actors dealing with the BWC directly or indirectly is crucial in the areas of disease surveillance, fighting chemical weapons, and opposing the threat of bioterrorism. In such a vision, the BWC will be key, both as a clear and fundamental legal norm, and as a forum for coordination of the various activities. Biological Weapons Convention will play an ever more important role in efforts to maximize the benefits offered by biotechnology while minimizing its potential for malign and hostile use.(author)

  6. Transparency to what End? Governing by disclosure through the Biosafety Clearing House

    NARCIS (Netherlands)

    Gupta, A.

    2010-01-01

    Although transparency is a key concept in the social sciences, it remains an understudied phenomenon in global environmental governance. This paper analyzes effectiveness of ‘governance by transparency’ or governance by information disclosure as a key innovation in global environmental and risk

  7. Application of Transgenic Technologies to Papaya: Developments and Biosafety Assessments in Thailand

    Czech Academy of Sciences Publication Activity Database

    Kertbundit, Sunee; Juříček, Miloslav

    2010-01-01

    Roč. 4, č. 1 (2010), s. 52-57 ISSN 1749-0413 Institutional research plan: CEZ:AV0Z50380511 Keywords : coat protein-mediated resistance * GMO * Papaya ringspot virus Subject RIV: EF - Botanics http://home.ueb.cas.cz/publikace/2010_Kertbundit_TransgenicPlantJournal_52.pdf

  8. Specified assurance level sampling procedure

    International Nuclear Information System (INIS)

    Willner, O.

    1980-11-01

    In the nuclear industry design specifications for certain quality characteristics require that the final product be inspected by a sampling plan which can demonstrate product conformance to stated assurance levels. The Specified Assurance Level (SAL) Sampling Procedure has been developed to permit the direct selection of attribute sampling plans which can meet commonly used assurance levels. The SAL procedure contains sampling plans which yield the minimum sample size at stated assurance levels. The SAL procedure also provides sampling plans with acceptance numbers ranging from 0 to 10, thus, making available to the user a wide choice of plans all designed to comply with a stated assurance level

  9. Digital Levelling in Subterranean Spaces

    Directory of Open Access Journals (Sweden)

    Tomáš Jiřikovský

    2007-06-01

    Full Text Available For precision levelling works are now more often used digital levels and code-scale staffs. Advantages in (and problems with their application to the regular line-levelling are well known and described. However, when using the digital levelling for measurements in specific local geodetic networks, monitoring networks and inside of buildings and underground spaces, new problems appear with the signalisation of the observed points, readability of the code (non-uniform illumination, temperature changes etc. The article informs about the application of two types of digital levels (Sokkia SDL-2, Trimble Zeiss DiNi 12T in the experimental subterranean levelling network for the basement settlement monitoring of a ten-floor building; the solution of marking of the points, field calibration and the system calibration of digital levels.

  10. Liquid level measurement in high level nuclear waste slurries

    International Nuclear Information System (INIS)

    Weeks, G.E.; Heckendorn, F.M.; Postles, R.L.

    1990-01-01

    Accurate liquid level measurement has been a difficult problem to solve for the Defense Waste Processing Facility (DWPF). The nuclear waste sludge tends to plug or degrade most commercially available liquid-level measurement sensors. A liquid-level measurement system that meets demanding accuracy requirements for the DWPF has been developed. The system uses a pneumatic 1:1 pressure repeater as a sensor and a computerized error correction system. 2 figs

  11. 76 FR 63891 - Foreign Quarantine; Etiological Agents, Hosts, and Vectors

    Science.gov (United States)

    2011-10-14

    ... applicants possess written biosafety plans and already follow standard biosafety practices and procedures... CFR 71.54 A. Section Heading & Definitions B. Biosafety and Inspection Provisions [[Page 63892

  12. 'Level-level correlation and absorption in nuclear reactions'

    International Nuclear Information System (INIS)

    Hussein, M.S.

    Level-level correlation (LLC) in nuclear reactions is discussed in general and it is shown that in the presence of LLC, N sub(μ) = Σ/g μa/ 2 > divided by gamma μ T tilde, where T tilde is the average absorption in the eigen channels [pt

  13. Comments About a Chameleon Theory: Level I/Level II.

    Science.gov (United States)

    Horn, John; Stankov, Lazar

    1982-01-01

    Jensen's ideas about two levels of intellectual abilities are criticized as being oversimplified. More than two levels of intellectual abilities and relationships between variables reflecting more than racial and socioeconomic status (SES) differences are suggested, arguing that Jensen's statements about race and SES differences are not properly…

  14. Low-level waste management

    International Nuclear Information System (INIS)

    Levin, G.B.

    1980-01-01

    An overview of the current situation in the United States and a look to the future of low-level waste management are presented. Current problems and challenges are discussed, such as: the need of additional disposal sites in the future; risks and costs involved in transport of low-level wastes; reduction of low-level waste volume through smelting, incineration, and storage for wastes containing nuclides with short half lives; development of a national policy for the management of low-level waste, and its implementation through a sensible system of regulations. Establishing a success with low-level waste management should provide the momentum and public confidence needed to continue on and to resolve the technical and politically more difficult low-level waste problems

  15. Culture at the Country Level

    OpenAIRE

    Maseland, R.K.J.; Hoorn, A.A.J. van; Herk, H. van; Torelli, C.J.

    2017-01-01

    This chapter introduces and critically discusses the idea of measuring the culture of countries and cross-national differences therein. We start by elaborating the theoretical foundations for studying culture at the country level. We highlight the use of countries or nations as a unit of analysis and pay special attention to the way in which a group-level construct such as culture has implications at lower levels of analysis, affecting the values and beliefs of individuals. After briefly trac...

  16. Nucleonic level control in industries

    International Nuclear Information System (INIS)

    Eapen, A.C.; Rao, S.S.; Nair, R.S.

    1979-01-01

    The paper elicits the particular advantages of nucleonic level control methods in certain applications. The technique used is described briefly. Normal radioactive sources used and their selection for any application based on their characteristic emissions is explained. Level controller developed by the Bhabha Atomic Research Centre, Bombay, is described including details regarding electronic circuitry and the radiation detector used. Its application in zinc powder plant to measure powder level in a hopper is described. Some of the typical applications of gamma-ray level controllers are mentioned. (auth.)

  17. On 165Ho level scheme

    International Nuclear Information System (INIS)

    Ardisson, Claire; Ardisson, Gerard.

    1976-01-01

    A 165 Ho level scheme was constructed which led to the interpretation of sixty γ rays belonging to the decay of 165 Dy. A new 702.9keV level was identified to be the 5/2 - member of the 1/2 ) 7541{ Nilsson orbit. )] [fr

  18. Background noise levels in Europe

    OpenAIRE

    Gjestland, Truls

    2008-01-01

    - This report gives a brief overview of typical background noise levels in Europe, and suggests a procedure for the prediction of background noise levels based on population density. A proposal for the production of background noise maps for Europe is included.

  19. Sea Level Rise Data Discovery

    Science.gov (United States)

    Quach, N.; Huang, T.; Boening, C.; Gill, K. M.

    2016-12-01

    Research related to sea level rise crosses multiple disciplines from sea ice to land hydrology. The NASA Sea Level Change Portal (SLCP) is a one-stop source for current sea level change information and data, including interactive tools for accessing and viewing regional data, a virtual dashboard of sea level indicators, and ongoing updates through a suite of editorial products that include content articles, graphics, videos, and animations. The architecture behind the SLCP makes it possible to integrate web content and data relevant to sea level change that are archived across various data centers as well as new data generated by sea level change principal investigators. The Extensible Data Gateway Environment (EDGE) is incorporated into the SLCP architecture to provide a unified platform for web content and science data discovery. EDGE is a data integration platform designed to facilitate high-performance geospatial data discovery and access with the ability to support multi-metadata standard specifications. EDGE has the capability to retrieve data from one or more sources and package the resulting sets into a single response to the requestor. With this unified endpoint, the Data Analysis Tool that is available on the SLCP can retrieve dataset and granule level metadata as well as perform geospatial search on the data. This talk focuses on the architecture that makes it possible to seamlessly integrate and enable discovery of disparate data relevant to sea level rise.

  20. Sea level and climate variations

    NARCIS (Netherlands)

    Oerlemans, J.

    1985-01-01

    Review paper, ESA Symposium on Application of Satellite Data to Climate Modelling. Alpbach (Austria) Sea level is an essential component of the climate system, on which many human activities in the coastal zone depend. Climate variations leading to changes in relative sea level are

  1. Enhanced Waste Tank Level Model

    Energy Technology Data Exchange (ETDEWEB)

    Duignan, M.R.

    1999-06-24

    'With the increased sensitivity of waste-level measurements in the H-Area Tanks and with periods of isolation, when no mass transfer occurred for certain tanks, waste-level changes have been recorded with are unexplained.'

  2. Evaluations of average level spacings

    International Nuclear Information System (INIS)

    Liou, H.I.

    1980-01-01

    The average level spacing for highly excited nuclei is a key parameter in cross section formulas based on statistical nuclear models, and also plays an important role in determining many physics quantities. Various methods to evaluate average level spacings are reviewed. Because of the finite experimental resolution, to detect a complete sequence of levels without mixing other parities is extremely difficult, if not totally impossible. Most methods derive the average level spacings by applying a fit, with different degrees of generality, to the truncated Porter-Thomas distribution for reduced neutron widths. A method that tests both distributions of level widths and positions is discussed extensivey with an example of 168 Er data. 19 figures, 2 tables

  3. Noise levels in Damascus city

    International Nuclear Information System (INIS)

    Al-Oudat, M.; Maslmani, Y.

    2004-01-01

    Outdoor noise levels were measured at 22 sites in Damascus city. Sound level meter model NC-10 with a 20-140 dBA selectable range was used in the current investigation. At each site noise data were collected from 7 to 21 o'clock. The results showed that the noise levels were higher than WHO (World Health Organization) standard by 5-24.7 dB, 10-16 dB, 10-11 dB and 12-17 dB in residential, commercial, Commercial-industrial, and Heavy traffic streets respectively. Indoor and outdoor noise levels in some hospitals were higher than WHO standard by 15-28 dB and 19-23 dB respectively. The study showed that the authorities administration must take necessary procedures to reduce the noise levels in residential regions and in the regions surrounding the hospitals. (author)

  4. The CDF level-3 trigger

    International Nuclear Information System (INIS)

    Devlin, T.

    1993-01-01

    The Collider Detector at Fermilab (CDF) has been operating at the Tevatron and collecting data on proton-antiproton interactions with collision rates above 250,000 Hz. Three levels of filtering select events for data logging at a rate of about 4 Hz. The Level 3 trigger provides most of the capabilities of the offline production programs for event reconstruction and physics analysis. The type of physics triggers, application of cuts, and combinations of logical requirements for event selection are controlled at run time by a trigger table using a syntax fully integrated with the Level 1 and Level 2 hardware triggers. The level 3 software operates in 48 RISC/UNIX processors (over 1000 mips) served by four 20-MByte/sec data buses for input, output and control. The system architecture, debugging, code validation, error reporting, analysis capabilities and performance will be described

  5. Mycobacterium tuberculosis and Mycobacterium marinum non-homologous end-joining proteins can function together to join DNA ends in Escherichia coli.

    Science.gov (United States)

    Wright, Douglas G; Castore, Reneau; Shi, Runhua; Mallick, Amrita; Ennis, Don G; Harrison, Lynn

    2017-03-01

    Mycobacterium tuberculosis and Mycobacterium smegmatis express a Ku protein and a DNA ligase D and are able to repair DNA double strand breaks (DSBs) by non-homologous end-joining (NHEJ). This pathway protects against DNA damage when bacteria are in stationary phase. Mycobacterium marinum is a member of this mycobacterium family and like M. tuberculosis is pathogenic. M. marinum lives in water, forms biofilms and infects fish and frogs. M. marinum is a biosafety level 2 (BSL2) organism as it can infect humans, although infections are limited to the skin. M. marinum is accepted as a model to study mycobacterial pathogenesis, as M. marinum and M. tuberculosis are genetically closely related and have similar mechanisms of survival and persistence inside macrophage. The aim of this study was to determine whether M. marinum could be used as a model to understand M. tuberculosis NHEJ repair. We identified and cloned the M. marinum genes encoding NHEJ proteins and generated E. coli strains that express the M. marinum Ku (Mm-Ku) and ligase D (Mm-Lig) individually or together (LHmKumLig strain) from expression vectors integrated at phage attachment sites in the genome. We demonstrated that Mm-Ku and Mm-Lig are both required to re-circularize Cla I-linearized plasmid DNA in E. coli. We compared repair of strain LHmKumLig with that of an E. coli strain (BWKuLig#2) expressing the M. tuberculosis Ku (Mt-Ku) and ligase D (Mt-Lig), and found that LHmKumLig performed 3.5 times more repair and repair was more accurate than BWKuLig#2. By expressing the Mm-Ku with the Mt-Lig, or the Mt-Ku with the Mm-Lig in E. coli, we have shown that the NHEJ proteins from M. marinum and M. tuberculosis can function together to join DNA DSBs. NHEJ repair is therefore conserved between the two species. Consequently, M. marinum is a good model to study NHEJ repair during mycobacterial pathogenesis. © The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen

  6. Identification of potentially safe promising fungal cell factories for the production of polyketide natural food colorants using chemotaxonomic rationale

    Directory of Open Access Journals (Sweden)

    Frisvad Jens C

    2009-04-01

    Full Text Available Abstract Background Colorants derived from natural sources look set to overtake synthetic colorants in market value as manufacturers continue to meet the rising demand for clean label ingredients – particularly in food applications. Many ascomycetous fungi naturally synthesize and secrete pigments and thus provide readily available additional and/or alternative sources of natural colorants that are independent of agro-climatic conditions. With an appropriately selected fungus; using in particular chemotaxonomy as a guide, the fungal natural colorants could be produced in high yields by using the optimized cultivation technology. This approach could secure efficient production of pigments avoiding use of genetic manipulation. Results Polyketide pigment producing ascomycetous fungi were evaluated for their potential as production organisms based on a priori knowledge on species-specific pigment and potential mycotoxin production and BioSafety level (BSL classification. Based on taxonomic knowledge, we pre-selected ascomycetous fungi belonging to Penicillium subgenus Biverticillium that produced yellow, orange or red pigments while deselecting Penicillium marneffei; a well known human pathogen in addition to other mycotoxigenic fungi belonging to the same group. We identified 10 strains belonging to 4 species; viz. P. purpurogenum, P. aculeatum, P. funiculosum, and P. pinophilum as potential pigment producers that produced Monascus-like pigments but no known mycotoxins. The selection/deselection protocol was illustrated in the pigment extracts of P. aculeatum IBT 14259 and P. crateriforme IBT 5015 analysed by HPLC-DAD-MS. In addition, extracellular pigment producing ability of some of the potential pigment producers was evaluated in liquid media with a solid support and N-glutarylmonascorubramine was discovered in the partially purified pigment extract of P. purpurogenum IBT 11181 and IBT 3645. Conclusion The present work brought out that the use

  7. Hypoglycemic and anti-hyperglycemic activity of Triphalādi granules in mice

    Directory of Open Access Journals (Sweden)

    Ankush Gunjal

    2016-01-01

    Conclusion: Triphalādi granules have significant hypoglycemic and anti-hyperglycemic activity in mice with minimal effect on BSL below normal range. The studies suggest the potential role of Triphalādi granules as an alternative adjuvant therapy in diabetic individuals for the control of the blood glucose level.

  8. Relationships between the school-level and classroom-level ...

    African Journals Online (AJOL)

    education; school-level environment; science education; South Africa. Introduction .... instrument to the primary school students (N = 1,077) of 31 distance-education primary school teachers ..... Centre for Curriculum, Transfer and Technology.

  9. The Barbados Sea Level Record

    Science.gov (United States)

    Fairbanks, R. G.; Mortlock, R. A.; Abdul, N. A.; Wright, J. D.; Cao, L.; Mey, J. L.

    2013-12-01

    Additional offshore drill cores, nearly 100 new radiometric dates, and more than 1000 kilometers of Multibeam mapping greatly enhance the Barbados Sea Level record. Extensive Multibeam mapping around the entire island covers approximately 2650 km2 of the sea bottom and now integrates the offshore reef topography and Barbados Sea Level Record with the unparalleled onshore core collection, digital elevation maps, and Pleistocene sea level record spanning the past one million years. The reef crest coral, Acropora palmata, remains the stalwart indicator of sea level for many reasons that are validated by our redundant sea level records and redundant dating via Th/U and Pa/U analyses. Microanalysis and densitometry studies better explain why Acropora palmata is so well preserved in the Pleistocene reef records and therefore why it is the species of choice for sea level reconstructions and radiometric dating. New drill cores into reefs that formed during Marine Isotope Stage 3 lead us to a model of diagenesis that allows us to better prospect for unaltered coral samples in older reefs that may be suitable for Th/U dating. Equally important, our diagenesis model reinforces our rigorous sample quality criteria in a more quantitative manner. The Barbados Sea Level record has a sampling resolution of better than 100 years throughout much of the last deglaciation showing unprecedented detail in redundant drill cores. The Melt Water Pulses (MWP1A and MWP1B) are well resolved and the intervening interval that includes the Younger Dryas reveals sea level changes in new detail that are consistent with the terrestrial records of ice margins (see Abdul et al., this section). More than 100 paired Th/U and radiocarbon ages place the Barbados Sea Level Record unambiguously on the radiocarbon time scale for direct comparisons with the terrestrial records of ice margin changes.

  10. Optimum Safety Levels for Breakwaters

    DEFF Research Database (Denmark)

    Burcharth, H. F.; Sørensen, John Dalsgaard

    2005-01-01

    Optimum design safety levels for rock and cube armoured rubble mound breakwaters without superstructure are investigated by numerical simulations on the basis of minimization of the total costs over the service life of the structure, taking into account typical uncertainties related to wave...... statistics and structure response. The study comprises the influence of interest rate, service lifetime, downtime costs and damage accumulation. Design limit states and safety classes for breakwaters are discussed. The results indicate that optimum safety levels are somewhat higher than the safety levels...

  11. Farmers as Employers. Numeracy. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Tully, Chris

    This publication contains the three numeracy units of the three levels of Support Materials for Agricultural Training (SMAT) in the area of farmers as employers: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her numeracy skills needed to deal with employment of agriculture…

  12. Farm Management and Leadership. Numeracy. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Gadd, Nick; Lucas, Michele

    This publication contains the three numeracy units of the three levels of Support Materials for Agricultural Training (SMAT) in farm management and leadership: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her numeracy skills needed to deal with farm management. SMAT materials can…

  13. Occupational Health and Safety. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Gadd, Nick; Lucas, Michele

    This publication contains the three communication skills units of the three levels of Support Materials for Agricultural Training (SMAT) in the area of occupational health and safety: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her written and spoken communication skills needed…

  14. Occupational Health and Safety. Numeracy. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Tully, Chris

    This publication contains the three numeracy units of the three levels of Support Materials for Agricultural Training (SMAT) in the area of occupational health and safety: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her numeracy skills needed to deal with occupational safety and…

  15. Farmers as Employers. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Gadd, Nick; Lucas, Michele

    This publication contains the three communication skills units of the three levels of Support Materials for Agricultural Training (SMAT) in the area of farmers as employers: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her written and spoken communication and numeracy skills…

  16. Agricultural Production. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Gadd, Nick; Lucas, Michele

    This publication contains the three communication skills units of the three levels of Support Materials for Agricultural Training (SMAT) in agricultural production: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her written and spoken communication skills needed to deal with…

  17. Agricultural Production. Numeracy. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Tully, Chris

    This publication contains the three numeracy units of the three levels of Support Materials for Agricultural Training (SMAT) in agricultural production: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner improve his or her numeracy skills needed to deal with agricultural production. SMAT materials…

  18. Farm Management and Leadership. Level 1. Level 2. Level 3. Support Materials for Agricultural Training.

    Science.gov (United States)

    Batman, Kangan; Gadd, Nick; Lucas, Michele

    This publication contains the three communication skills units of the three levels of Support Materials for Agricultural Training (SMAT) in farm management and leadership: Level 1 (starting), 2 (continuing), and 3 (completing). The units are designed to help the learner with the reading, writing, and spoken communication skills needed to deal with…

  19. Acceptance criteria for deposition of low-level and intermediate-level radiation levels radioactive wastes

    International Nuclear Information System (INIS)

    2002-09-01

    This norm establishes the criteria for acceptance low and intermediate radiation level for safe deposition in repositories, for assuring the protection of workers, population and environment against the hazardous effects of the ionizing radiations. The criteria of this norm applies to the low and intermediate radiation levels

  20. Level best : drilling seasonal load leveling business case : final report

    International Nuclear Information System (INIS)

    2005-10-01

    Drilling and seismic costs in Western Canada are as much as 35 per cent higher during winter months. Favourable commodity pricing has prompted aggressive expansion of drilling programs in the Western Canada Sedimentary Basin (WCSB), and capacity is becoming a significant issue. Many operators are turning to load leveling in order to secure rigs and crew availability. However, many operators still adhere to the traditional view that drilling of oil and gas wells is best accomplished during the winter. The purpose of the project discussed in this paper was to develop and present a business case for greater seasonal load leveling in the WCSB and spreading it more evenly throughout the year. Using calendar year quarters, publicly available data for a 5 year period from 2000-2004 for drilling activity, safety, weather, and environmental restrictions were examined. A list of candidate fields with high levels of activity and a good representation of well depths was selected. Well cost and rig data from 14 different companies was used. A variety of industry participants, drilling contractors, load leveling technology suppliers and provincial agencies were consulted to supplement well cost analyses. The financial impacts of load leveling were examined. Benefits included lower well costs; advanced production; avoided rig construction; reduced incident rates; and reduced turnover. While the focus of the business case for load leveling was based on hard cost data, many indirect benefits were identified, including First Nations sustainability and greater job security and comfort for workers. Various policies and incentives that encouraged load leveling in British Columbia and Alberta were reviewed. 57 figs

  1. Atomic-level computer simulation

    International Nuclear Information System (INIS)

    Adams, J.B.; Rockett, Angus; Kieffer, John; Xu Wei; Nomura, Miki; Kilian, K.A.; Richards, D.F.; Ramprasad, R.

    1994-01-01

    This paper provides a broad overview of the methods of atomic-level computer simulation. It discusses methods of modelling atomic bonding, and computer simulation methods such as energy minimization, molecular dynamics, Monte Carlo, and lattice Monte Carlo. ((orig.))

  2. High-level-waste immobilization

    International Nuclear Information System (INIS)

    Crandall, J.L.

    1982-01-01

    Analysis of risks, environmental effects, process feasibility, and costs for disposal of immobilized high-level wastes in geologic repositories indicates that the disposal system safety has a low sensitivity to the choice of the waste disposal form

  3. MOVES regional level sensitivity analysis

    Science.gov (United States)

    2012-01-01

    The MOVES Regional Level Sensitivity Analysis was conducted to increase understanding of the operations of the MOVES Model in regional emissions analysis and to highlight the following: : the relative sensitivity of selected MOVES Model input paramet...

  4. Association in Level 2 Fusion

    National Research Council Canada - National Science Library

    Kokar, Mieczyslaw M; Matheus, Christopher J; Letkowski, Jerzy A; Baclawski, Kenneth; Kogut, Paul

    2004-01-01

    ... system, this cannot be said about the number of possible relations, which can grow exponentially. To alleviate the problems of computational complexity in Level 2 processing, the authors of this paper have suggested the use of ontologies...

  5. Processing TES Level-2 Data

    Science.gov (United States)

    Poosti, Sassaneh; Akopyan, Sirvard; Sakurai, Regina; Yun, Hyejung; Saha, Pranjit; Strickland, Irina; Croft, Kevin; Smith, Weldon; Hoffman, Rodney; Koffend, John; hide

    2006-01-01

    TES Level 2 Subsystem is a set of computer programs that performs functions complementary to those of the program summarized in the immediately preceding article. TES Level-2 data pertain to retrieved species (or temperature) profiles, and errors thereof. Geolocation, quality, and other data (e.g., surface characteristics for nadir observations) are also included. The subsystem processes gridded meteorological information and extracts parameters that can be interpolated to the appropriate latitude, longitude, and pressure level based on the date and time. Radiances are simulated using the aforementioned meteorological information for initial guesses, and spectroscopic-parameter tables are generated. At each step of the retrieval, a nonlinear-least-squares- solving routine is run over multiple iterations, retrieving a subset of atmospheric constituents, and error analysis is performed. Scientific TES Level-2 data products are written in a format known as Hierarchical Data Format Earth Observing System 5 (HDF-EOS 5) for public distribution.

  6. Intermediate Levels of Visual Processing

    National Research Council Canada - National Science Library

    Nakayama, Ken

    1998-01-01

    ...) surface representation, here we have shown that there is an intermediate level of visual processing, between the analysis of the image and higher order representations related to specific objects; (2...

  7. Nuclear systems of level measurement

    International Nuclear Information System (INIS)

    Lara, A.J.; Cabrera, M.J.

    1992-01-01

    In the industry there are processes in which is necessary to maintain the products level controlled which are handled for their transformation. The majority of such processes and by the operation conditions, they do not admit measure systems of level of invasive type then the application of nuclear techniques for level measurement results a big aid in these cases, since all the system installation is situated beyond frontiers of vessels that contain the product for measuring. In the Department of Nuclear Technology Applications of Mexican Petroleum Institute was developed a level measurement system by gamma rays transmission which operates in the Low Density Polyethylene plant of Petrochemical Complex Escolin at Poza Rica, Veracruz, Mexico. (Author)

  8. Levels of radioactivity in Qatar

    International Nuclear Information System (INIS)

    Al-Thani, A.A.; Abdul-Majid, S.; Mohammed, K.

    1995-01-01

    The levels of natural and man-made radioactivity in soil and seabed were measured in Qatar to assess radiation exposure levels and to evaluate any radioactive contamination that may have reached the country from fallout or due to the Chernobyl accident radioactivity release. Qatar peninsula is located on the Arabian Gulf, 4500 km from Chernobyl, and has an area of ∼11,600 km 2 and a population of ∼600,000

  9. Level structure in 123Xe

    International Nuclear Information System (INIS)

    Luukko, A.; Hattula, J.; Helppi, H.; Knuuttila, O.

    1980-09-01

    The level structure of 123 Xe has been studied with 122 Te( 3 He,2n) and 123 Te( 3 He,3n) reactions using in-beam γ-ray spectroscopic methods. Band-like level structures based on the unique-parity hsub(11/2) neutron state and different low-j states are observed. The isomeric state proposed earlier is established at 185.4 keV with a half-life of 5.6+-0.3 μs, and a new spin assignment of 7/2 - is proposed for this level. A triaxial-rotor-model calculation has been performed to interprete the negative-parity level system. Because of the 7/2 - state, we do not obtain a consistent description of the negative-parity states with the triaxial rotor model, unlike the heavier odd-A Xe nuclei. On the other hand, the positive-parity level structures are interpreted in terms of the core-quasiparticle model supposing a vibrational core. This indicates different core shapes for the positive- and negative-parity level systems. (author)

  10. Intermittent sea-level acceleration

    Science.gov (United States)

    Olivieri, M.; Spada, G.

    2013-10-01

    Using instrumental observations from the Permanent Service for Mean Sea Level (PSMSL), we provide a new assessment of the global sea-level acceleration for the last ~ 2 centuries (1820-2010). Our results, obtained by a stack of tide gauge time series, confirm the existence of a global sea-level acceleration (GSLA) and, coherently with independent assessments so far, they point to a value close to 0.01 mm/yr2. However, differently from previous studies, we discuss how change points or abrupt inflections in individual sea-level time series have contributed to the GSLA. Our analysis, based on methods borrowed from econometrics, suggests the existence of two distinct driving mechanisms for the GSLA, both involving a minority of tide gauges globally. The first effectively implies a gradual increase in the rate of sea-level rise at individual tide gauges, while the second is manifest through a sequence of catastrophic variations of the sea-level trend. These occurred intermittently since the end of the 19th century and became more frequent during the last four decades.

  11. Serum gonadotropins levels in childhood

    International Nuclear Information System (INIS)

    Hertogh, R. De; Vankrieken, L.; Wolter, R.; Vliet, G. Van

    1989-01-01

    The complex changes in serum LH and FSH levels from infancy to adulthood are diversely evaluated by radioimmunoassays or bioassays. The relative lack of sensitivity and specificity of radioimmunoassay, using polyclonal antibodies, could possibly be overcome by new immunoradiometric assays, using specific antibodies to LH and FSH. Significant differences were indeed observed between radioimmunoassays and immunoradiometric assays. During the prepubertal period, LH levels, measured by the immunoradiometric assays, were below the sensitivity of the method in the majority of the samples. LH levels were, however, well detectable when measured with radioimmunoassay, showing the heterogeneity of circulating LH structures. At the onset of puberty, LH levels increased at least 3 to 4 times in both sexes, when measured with immunoradiometric assays, whereas their increase was only 20 to 60% with the radioimmunoassays. FSH levels remained well detectable in the prepubertal period whether measured by immunoradiometric or radioimmunoassays. At pubertal onset, FSH increase in both sexes was more important in the immunoradiometric assays. The results obtained with immunoradiometric assays give a better insight into the quantitative and qualitative function of the gonadotropes during childhood. The almost complete absence of LH during the prepubertal period and the steep increase at the onset of puberty better reflects the reported data obtained with bioassays. The persistance of significant levels of FSH in the prepubertal ages, and the lesser increase at the onset of puberty, when compared with LH, illustrates that the individual regulation of LH and FSH secretion vary over time and is influenced by developmental factors. (author)

  12. Reactor water level control device

    International Nuclear Information System (INIS)

    Utagawa, Kazuyuki.

    1993-01-01

    A device of the present invention can effectively control fluctuation of a reactor water level upon power change by reactor core flow rate control operation. That is, (1) a feedback control section calculates a feedwater flow rate control amount based on a deviation between a set value of a reactor water level and a reactor water level signal. (2) a feed forward control section forecasts steam flow rate change based on a reactor core flow rate signal or a signal determining the reactor core flow rate, to calculate a feedwater flow rate control amount which off sets the steam flow rate change. Then, the sum of the output signal from the process (1) and the output signal from the process (2) is determined as a final feedwater flow rate control signal. With such procedures, it is possible to forecast the steam flow rate change accompanying the reactor core flow rate control operation, thereby enabling to conduct preceding feedwater flow rate control operation which off sets the reactor water level fluctuation based on the steam flow rate change. Further, a reactor water level deviated from the forecast can be controlled by feedback control. Accordingly, reactor water level fluctuation upon power exchange due to the reactor core flow rate control operation can rapidly be suppressed. (I.S.)

  13. Low-level toxicity of chemicals: No acceptable levels?

    Directory of Open Access Journals (Sweden)

    Bruce P Lanphear

    2017-12-01

    Full Text Available Over the past 3 decades, in a series of studies on some of the most extensively studied toxic chemicals and pollutants, scientists have found that the amount of toxic chemical linked with the development of a disease or death-which is central to determining "safe" or "hazardous" levels-is proportionately greater at the lowest dose or levels of exposure. These results, which are contrary to the way the United States Environmental Protection Agency (EPA and other regulatory agencies assess the risk of chemicals, indicate that we have underestimated the impact of toxic chemicals on death and disease. If widely disseminated chemicals and pollutants-like radon, lead, airborne particles, asbestos, tobacco, and benzene-do not exhibit a threshold and are proportionately more toxic at the lowest levels of exposure, we will need to achieve near-zero exposures to protect public health.

  14. Promoting system-level learning from project-level lessons

    International Nuclear Information System (INIS)

    Jong, Amos A. de; Runhaar, Hens A.C.; Runhaar, Piety R.; Kolhoff, Arend J.; Driessen, Peter P.J.

    2012-01-01

    A growing number of low and middle income nations (LMCs) have adopted some sort of system for environmental impact assessment (EIA). However, generally many of these EIA systems are characterised by a low performance in terms of timely information dissemination, monitoring and enforcement after licencing. Donor actors (such as the World Bank) have attempted to contribute to a higher performance of EIA systems in LMCs by intervening at two levels: the project level (e.g. by providing scoping advice or EIS quality review) and the system level (e.g. by advising on EIA legislation or by capacity building). The aims of these interventions are environmental protection in concrete cases and enforcing the institutionalisation of environmental protection, respectively. Learning by actors involved is an important condition for realising these aims. A relatively underexplored form of learning concerns learning at EIA system-level via project level donor interventions. This ‘indirect’ learning potentially results in system changes that better fit the specific context(s) and hence contribute to higher performances. Our exploratory research in Ghana and the Maldives shows that thus far, ‘indirect’ learning only occurs incidentally and that donors play a modest role in promoting it. Barriers to indirect learning are related to the institutional context rather than to individual characteristics. Moreover, ‘indirect’ learning seems to flourish best in large projects where donors achieved a position of influence that they can use to evoke reflection upon system malfunctions. In order to enhance learning at all levels donors should thereby present the outcomes of the intervention elaborately (i.e. discuss the outcomes with a large audience), include practical suggestions about post-EIS activities such as monitoring procedures and enforcement options and stimulate the use of their advisory reports to generate organisational memory and ensure a better information

  15. Promoting system-level learning from project-level lessons

    Energy Technology Data Exchange (ETDEWEB)

    Jong, Amos A. de, E-mail: amosdejong@gmail.com [Innovation Management, Utrecht (Netherlands); Runhaar, Hens A.C., E-mail: h.a.c.runhaar@uu.nl [Section of Environmental Governance, Utrecht University, Utrecht (Netherlands); Runhaar, Piety R., E-mail: piety.runhaar@wur.nl [Organisational Psychology and Human Resource Development, University of Twente, Enschede (Netherlands); Kolhoff, Arend J., E-mail: Akolhoff@eia.nl [The Netherlands Commission for Environmental Assessment, Utrecht (Netherlands); Driessen, Peter P.J., E-mail: p.driessen@geo.uu.nl [Department of Innovation and Environment Sciences, Utrecht University, Utrecht (Netherlands)

    2012-02-15

    A growing number of low and middle income nations (LMCs) have adopted some sort of system for environmental impact assessment (EIA). However, generally many of these EIA systems are characterised by a low performance in terms of timely information dissemination, monitoring and enforcement after licencing. Donor actors (such as the World Bank) have attempted to contribute to a higher performance of EIA systems in LMCs by intervening at two levels: the project level (e.g. by providing scoping advice or EIS quality review) and the system level (e.g. by advising on EIA legislation or by capacity building). The aims of these interventions are environmental protection in concrete cases and enforcing the institutionalisation of environmental protection, respectively. Learning by actors involved is an important condition for realising these aims. A relatively underexplored form of learning concerns learning at EIA system-level via project level donor interventions. This 'indirect' learning potentially results in system changes that better fit the specific context(s) and hence contribute to higher performances. Our exploratory research in Ghana and the Maldives shows that thus far, 'indirect' learning only occurs incidentally and that donors play a modest role in promoting it. Barriers to indirect learning are related to the institutional context rather than to individual characteristics. Moreover, 'indirect' learning seems to flourish best in large projects where donors achieved a position of influence that they can use to evoke reflection upon system malfunctions. In order to enhance learning at all levels donors should thereby present the outcomes of the intervention elaborately (i.e. discuss the outcomes with a large audience), include practical suggestions about post-EIS activities such as monitoring procedures and enforcement options and stimulate the use of their advisory reports to generate organisational memory and ensure a better

  16. State level operations and interaction with facility level systems

    International Nuclear Information System (INIS)

    Bellinger, J.

    1989-01-01

    The role of the State System of Accounting for and Control of Nuclear Materials at the State level, particularly the role of the National Authority, in ensuring that both national and international safeguards objectives are met is discussed. The legislative basis for the National Authority is examined. The activities of Australia's National Authority - the Australian Safeguards Office - are described

  17. Level densities in nuclear physics

    International Nuclear Information System (INIS)

    Beckerman, M.

    1978-01-01

    In the independent-particle model nucleons move independently in a central potential. There is a well-defined set of single- particle orbitals, each nucleon occupies one of these orbitals subject to Fermi statistics, and the total energy of the nucleus is equal to the sum of the energies of the individual nucleons. The basic question is the range of validity of this Fermi gas description and, in particular, the roles of the residual interactions and collective modes. A detailed examination of experimental level densities in light-mass system is given to provide some insight into these questions. Level densities over the first 10 MeV or so in excitation energy as deduced from neutron and proton resonances data and from spectra of low-lying bound levels are discussed. To exhibit some of the salient features of these data comparisons to independent-particle (shell) model calculations are presented. Shell structure is predicted to manifest itself through discontinuities in the single-particle level density at the Fermi energy and through variatons in the occupancy of the valence orbitals. These predictions are examined through combinatorial calculations performed with the Grover [Phys. Rev., 157, 832(1967), 185 1303(1969)] odometer method. Before the discussion of the experimenta results, statistical mechanical level densities for spherical nuclei are reviewed. After consideration of deformed nuclei, the conclusions resulting from this work are drawn. 7 figures, 3 tables

  18. Space elevator systems level analysis

    Energy Technology Data Exchange (ETDEWEB)

    Laubscher, B. E. (Bryan E.)

    2004-01-01

    The Space Elevator (SE) represents a major paradigm shift in space access. It involves new, untried technologies in most of its subsystems. Thus the successful construction of the SE requires a significant amount of development, This in turn implies a high level of risk for the SE. This paper will present a systems level analysis of the SE by subdividing its components into their subsystems to determine their level of technological maturity. such a high-risk endeavor is to follow a disciplined approach to the challenges. A systems level analysis informs this process and is the guide to where resources should be applied in the development processes. It is an efficient path that, if followed, minimizes the overall risk of the system's development. systems level analysis is that the overall system is divided naturally into its subsystems, and those subsystems are further subdivided as appropriate for the analysis. By dealing with the complex system in layers, the parameter space of decisions is kept manageable. Moreover, A rational way to manage One key aspect of a resources are not expended capriciously; rather, resources are put toward the biggest challenges and most promising solutions. This overall graded approach is a proven road to success. The analysis includes topics such as nanotube technology, deployment scenario, power beaming technology, ground-based hardware and operations, ribbon maintenance and repair and climber technology.

  19. Nuclear level mixing resonance spectroscopy

    International Nuclear Information System (INIS)

    Coussement, R.; Put, P.; Scheveneels, G.; Hardeman, F.

    1985-01-01

    The existent methods for measuring quadrupole interactions are not suited to nuclei with lifetimes in the micro-seconds to minutes region. AD/NQR, a possible candidate in this lifetime gap, has not yet succeeded in overcoming its predicted difficulties. A new resonant method, recently developed and based on the principles of level mixing (cfr atomic spectroscopy) covers this less accessible lifetime range. Many other kinds of resonances can be described according to the level mixing formalism. The particular example of NMR as a level mixing resonance (LMR) is discussed. The underlying theory of LMR and its important consequences, leading to some interesting features of the method, is briefly formulated. Two successfully performed measurements demonstrate the feasibility and the predicted characteristics of this new promising method. (orig.)

  20. Low-level Radioactivity Measurements

    International Nuclear Information System (INIS)

    Churtgen, C.

    2007-01-01

    The low-level radioactivity measurements service performs measurements of alpha or beta emitters on various types of low-radioactivity samples (biological and environmental) from internal and external clients. to maintain and develop techniques concerning the measurement of low-level radioactivity of alpha and beta emitting radionuclides in environmental or biological samples; to measure these samples by means of low-background counters (liquid scintillators, proportional counters, ZnS counters and alpha-spectrometers); to support and advise the nuclear and non-nuclear industry on problems of radioactive contamination or low level radioactivity measurements; to maintain the quality assurance system according to the ISO17025 standard for which we obtained the Beltest accreditation in 1998; to assess the internal dose from occupational intakes of radionuclides for workers of the nuclear industry;

  1. High Level Radioactive Waste Management

    International Nuclear Information System (INIS)

    1991-01-01

    The proceedings of the second annual international conference on High Level Radioactive Waste Management, held on April 28--May 3, 1991, Las Vegas, Nevada, provides information on the current technical issue related to international high level radioactive waste management activities and how they relate to society as a whole. Besides discussing such technical topics as the best form of the waste, the integrity of storage containers, design and construction of a repository, the broader social aspects of these issues are explored in papers on such subjects as conformance to regulations, transportation safety, and public education. By providing this wider perspective of high level radioactive waste management, it becomes apparent that the various disciplines involved in this field are interrelated and that they should work to integrate their waste management activities. Individual records are processed separately for the data bases

  2. High-level Petri Nets

    DEFF Research Database (Denmark)

    various journals and collections. As a result, much of this knowledge is not readily available to people who may be interested in using high-level nets. Within the Petri net community this problem has been discussed many times, and as an outcome this book has been compiled. The book contains reprints...... of some of the most important papers on the application and theory of high-level Petri nets. In this way it makes the relevant literature more available. It is our hope that the book will be a useful source of information and that, e.g., it can be used in the organization of Petri net courses. To make......High-level Petri nets are now widely used in both theoretical analysis and practical modelling of concurrent systems. The main reason for the success of this class of net models is that they make it possible to obtain much more succinct and manageable descriptions than can be obtained by means...

  3. Low-level Radioactivity Measurements

    International Nuclear Information System (INIS)

    Hurtgen, C.

    2002-01-01

    The objectives of the research performed in the area of low-level radioactivity measurements are (1) to maintain and develop techniques for the measurement of low-level environmental and biological samples, (2) to measure these samples by means of low-background counters (liquid scintillators, proportional counters, ZnS counters, alpha spectrometry), (3) to support and advise the nuclear and non-nuclear industry on problems of radioactive contamination and low-level radioactivity measurements; (4) to maintain and improve the quality assurance system according to the ISO17025 standard; and (5) to assess the internal dose from occupational intakes of radionuclides of workers of the nuclear industry. Progress and achievements in these areas in 2001 are reported

  4. Integrated at the neighbourhood level

    DEFF Research Database (Denmark)

    Putri, Prathiwi Widyatmi

    2017-01-01

    Cities in the Global South are generally vast due to urban sprawl. They are characterised by a varying level of density, and enclaves of informal settlements. Within this context, this article addresses the limits of large-scale and centralised water systems. It seeks to understand, qualitatively......-spatial characteristics of local communities can be accommodated. Smaller-scale development intervention also means stimulating creativity in planning and policy-making processes to address water-infrastructure needs at local levels and opens possibilities for integrating water-infrastructures with public space....... Such a decentralised approach matters to improve the overall socio-spatial quality of a neighbourhood, however it requires, in parallel, new institutional mechanisms to provide a coherent water and environmental management system at the urban level. This article argues for a synergy of two axes: the water sector...

  5. Low-level Radioactivity Measurements

    International Nuclear Information System (INIS)

    Hurtgen, C.

    2001-01-01

    The objectives of the research performed in the area of low-level radioactivity measurements are (1) to maintain and develop techniques for the measurement of low-level environmental and biological samples, (2) to measure these samples by means of low-background counters (liquid scintillators, proportional counters, ZnS counters, alpha spectrometry), (3) to support and advice the nuclear and non-nuclear industry in matters concerning radioactive contamination and/or low-level radioactivity measurements; (4) to maintain the quality assurance system according to the EN45001/ISO17025 standard; and (5) to assess the internal dose from occupational intakes of radionuclides of workers of the nuclear industry. Progress and achievements in these areas in 2000 are reported

  6. Modeling Multi-Level Systems

    CERN Document Server

    Iordache, Octavian

    2011-01-01

    This book is devoted to modeling of multi-level complex systems, a challenging domain for engineers, researchers and entrepreneurs, confronted with the transition from learning and adaptability to evolvability and autonomy for technologies, devices and problem solving methods. Chapter 1 introduces the multi-scale and multi-level systems and highlights their presence in different domains of science and technology. Methodologies as, random systems, non-Archimedean analysis, category theory and specific techniques as model categorification and integrative closure, are presented in chapter 2. Chapters 3 and 4 describe polystochastic models, PSM, and their developments. Categorical formulation of integrative closure offers the general PSM framework which serves as a flexible guideline for a large variety of multi-level modeling problems. Focusing on chemical engineering, pharmaceutical and environmental case studies, the chapters 5 to 8 analyze mixing, turbulent dispersion and entropy production for multi-scale sy...

  7. Deafness and motor abilities level

    Directory of Open Access Journals (Sweden)

    A Zwierzchowska

    2008-09-01

    Full Text Available The audition injury hinders some motor motions and the organised coordination at the higher level and may be a cause of disturbances and disorder in some motor abilities adoption. It was assumed that deafness including its aetiology and injury mechanism may significantly influence the motor development of human being. The study aimed in checking if the deafness, as a result of various unfavourable factors, determines the motor development of children and youngsters. Consequently the dependency between qualitative features i.e.: signed motor level and aetiology, audition injury mechanism and the deafness degree was examined. The mechanism and aetiology of hearing correlated with the motor abilities displayed statistically significant dependencies in few motor trials only. Revealed correlations regarded mostly the coordination trials excluding the flexibility one. Statistically significant dependencies between the audition diminution and the motor abilities level were not found.

  8. Is sea-level rising?

    Digital Repository Service at National Institute of Oceanography (India)

    Unnikrishnan, A.S.

    correction in the estimation of trends obtained for tide gauge records. The altimeter data permits to prepare spatial maps of sea-level rise trends. We present a map prepared for the Indian Ocean (Figure 4) north of 10oS , which shows a fairly uniform... drawn information from research papers published by the author and report of the IPCC AR5 WG1 Chapter 13: Sea Level Changes, in which the author has served as a ‘Lead Author’. Figure1 is prepared using data from the University of Colorado. Nerem, R...

  9. Levels of Outsourcing and Offshoring

    DEFF Research Database (Denmark)

    Madsen, Erik Skov; Slepniov, Dmitrij

    2011-01-01

    The purpose of this paper is to examine the evolution of offshoring and outsourcing over time and highlight strategic considerations underpinning this process in companies. On the basis of four case studies of Danish and German industrial companies, this paper develops a holistic framework...... conceptualizing various levels of outsourcing and offshoring as well as factors driving the transition between these levels. The framework adds to better understanding of the process and challenges the linear nature of it and suggests that ‘backsourcing’ and repatriation of activities should receive more...

  10. Colloids related to low level and intermediate level waste

    International Nuclear Information System (INIS)

    Ramsay, J.D.F.; Russell, P.J.; Avery, R.G.

    1991-01-01

    A comprehensive research investigation has been undertaken to improve the understanding of the potential role of colloids in the context of disposal and storage of low level and intermediate level waste immobilized in cement. Several topics have been investigated which include: (a) the study of the formation and characteristics of colloids in cement leachates; (b) the effects of the near-field aqueous chemistry on the characteristics of colloids in repository environments; (c) colloid sorption behaviour; (d) interactions of near-field materials with leachates; (e) characteristics of near-field materials in EC repository simulation tests; and (f) colloid migration behaviour. These experimental investigations should provide data and a basis for the development of transport models and leaching mechanisms, and thus relate directly to the part of the Task 3 programme concerned with migration and retention of radionuclides in the near field. 114 Figs.; 39 Tabs.; 12 Refs

  11. High-Level Radioactive Waste.

    Science.gov (United States)

    Hayden, Howard C.

    1995-01-01

    Presents a method to calculate the amount of high-level radioactive waste by taking into consideration the following factors: the fission process that yields the waste, identification of the waste, the energy required to run a 1-GWe plant for one year, and the uranium mass required to produce that energy. Briefly discusses waste disposal and…

  12. Beyond macrocognition : The transaction level

    NARCIS (Netherlands)

    Schraagen, J.M.C.

    2017-01-01

    The purpose of this paper is to respond to a number of developments in the past 15 years in the areas of NDM and macrocognition. In my view, these developments necessitate the definition of a new systems level, extending and modifying Newell’s (1990) "bands of cognition". I will argue that what has

  13. Cultural Understanding: Spanish Level 1.

    Science.gov (United States)

    Scott, Reid

    The teacher's attention is focused on selected elements of Spanish culture which may be taught integrally with instructional materials found in the first-year Spanish texts "Entender y Hablar", "La Familia Fernandez", and "A-LM Spanish, Level One". Items are cross-referenced for 42 cultural concepts ranging from nicknames to streets, roads, and…

  14. Level Design as Model Transformation

    NARCIS (Netherlands)

    Dormans, Joris

    2011-01-01

    This paper frames the process of designing a level in a game as a series of model transformations. The transformations correspond to the application of particular design principles, such as the use of locks and keys to transform a linear mission into a branching space. It shows that by using rewrite

  15. Biocards and Level of Abstraction

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Keshwani, Sonal; Chakrabarti, Amaresh

    2015-01-01

    Biocards are formal descriptions of biological phenomena and their underlying functional principles. They are used in bioinspired design to document search results and to communicate the findings for use in the further design process. The present study explored the effect of abstraction level used...

  16. Time orientation and construal level

    NARCIS (Netherlands)

    Beek, van Jannette; Handgraaf, Michel J.J.; Antonides, Gerrit

    2017-01-01

    Eating and exercising behaviour are both characterized by immediate and future consequences. Consequently, consideration of these consequences (i.e. time orientation) predicts eating and exercising behaviour. We investigate whether construal level acts as an underlying mechanism of these

  17. Engineering at the Elementary Level

    Science.gov (United States)

    McGrew, Cheryl

    2012-01-01

    Can engineering technology be taught at the elementary level? Designing and building trebuchets, catapults, solar cars, and mousetrap vehicles in a west central Florida elementary class was considered very unusual in recent years. After a review of current research on failing schools and poor curriculum, the author wondered what her school could…

  18. Energy levels of 56Mn

    DEFF Research Database (Denmark)

    Van Assche, P. H. M.; Baader, H. A.; Koch, H. R.

    1971-01-01

    The low-energy spectrum of the 55Mn(n,γ)56 Mn reaction has been studied with a γ-diffraction spectrometer. These data allowed the construction of a level scheme for 56Mn with two previously unobserved doublets. High-energy γ-transitions to the low-energy states have been measured for different...

  19. Transportable type neutron level indicators

    International Nuclear Information System (INIS)

    Khatskevich, M.V.; Kalinin, O.V.; Moskovkin, V.N.; Molchanov, A.V.; Bobkov, A.D.; Rabotnov, Yu.A.

    1979-01-01

    Some peculiarities of designing level neutron converters (LNC) for portable indicators or level neutron relays are considered. The effect of the LNC geometry and other factors on measurement errors has been studied. Calibration results of the LNC with a neutron reflector and without it are presented. It is shown that the problem of level monitoring with the help of portable indicators can be solved practically for any volume, provided two LNC modifications with reflectors are available: the NPU-G modification with horizontal location of a counter for large volumes and the NPU-V with vertical location of a counter for lesser volumes. A possibility of perfecting LNC performances by shielding the counter with thermal neutron absorbers has been studied. The design of the NPU-V modification for the NIUP-2 level indicator is described. It is intended for tubes and cylinders 30-100 mm in diameter. Measurements carried out on different steel and aluminium vessels with a diameter ranging from 300 to 100 mm and a wall thickness of up to 16 mm with the help of the NPU-V and NPU-G modifications proved the efficiency of the LNC to control a variety of products (kerosine, gasoline, oils, acids, alkalis) [ru

  20. Visualizing attitudes towards service levels

    NARCIS (Netherlands)

    K.Y. Lam (Kar Yin); M. van de Velden (Michel); Ph.H.B.F. Franses (Philip Hans)

    2011-01-01

    textabstractTo assess the attitudes with respect to the quality of banks’ service levels, we use survey data amongst more than 250 Chief Financial Officers (CFOs) of a range of Netherlands-based companies. These companies range from small to very large (including multinationals as Philips and Shell)

  1. Selfishness Level of Strategic Games

    NARCIS (Netherlands)

    Apt, K.R.; Schäfer, G.

    2014-01-01

    We introduce a new measure of the discrepancy in strategic games between the social welfare in a Nash equilibrium and in a social optimum, that we call selfishness level. It is the smallest fraction of the social welfare that needs to be offered to each player to achieve that a social optimum is

  2. Logical Entity Level Sentiment Analysis

    DEFF Research Database (Denmark)

    Petersen, Niklas Christoffer; Villadsen, Jørgen

    2017-01-01

    We present a formal logical approach using a combinatory categorial grammar for entity level sentiment analysis that utilizes machine learning techniques for efficient syntactical tagging and performs a deep structural analysis of the syntactical properties of texts in order to yield precise resu...

  3. RPython high-level synthesis

    Science.gov (United States)

    Cieszewski, Radoslaw; Linczuk, Maciej

    2016-09-01

    The development of FPGA technology and the increasing complexity of applications in recent decades have forced compilers to move to higher abstraction levels. Compilers interprets an algorithmic description of a desired behavior written in High-Level Languages (HLLs) and translate it to Hardware Description Languages (HDLs). This paper presents a RPython based High-Level synthesis (HLS) compiler. The compiler get the configuration parameters and map RPython program to VHDL. Then, VHDL code can be used to program FPGA chips. In comparison of other technologies usage, FPGAs have the potential to achieve far greater performance than software as a result of omitting the fetch-decode-execute operations of General Purpose Processors (GPUs), and introduce more parallel computation. This can be exploited by utilizing many resources at the same time. Creating parallel algorithms computed with FPGAs in pure HDL is difficult and time consuming. Implementation time can be greatly reduced with High-Level Synthesis compiler. This article describes design methodologies and tools, implementation and first results of created VHDL backend for RPython compiler.

  4. Middle Level Learning Number 47

    Science.gov (United States)

    Lapham, Steven S.; Hanes, Peter; Turner, Thomas N.; Clabough, Jeremiah C.; Cole, William

    2013-01-01

    This issue's "Middle Level Learning" section presents two articles. The first is "Harriet Tubman: Emancipate Yourself!" (by Steven S. Lapham and Peter Hanes). "Argo," which won the 2012 Oscar for best picture, was about a daring escape of six U.S. diplomats from Iran during the 1979 hostage crisis. Now imagine the…

  5. Legitimizing differentiated flood protection levels

    NARCIS (Netherlands)

    Thomas, Hartmann; Spit, Tejo

    2016-01-01

    The European flood risk management plan is a new instrument introduced by the Floods Directive. It introduces a spatial turn and a scenario approach in flood risk management, ultimately leading to differentiated flood protection levels on a catchment basis. This challenges the traditional sources of

  6. Reduction of parathyoid hormone levels

    DEFF Research Database (Denmark)

    2011-01-01

    A method of acutely reducing the plasma level of PTH of a patient having an elevated PTH comprising the administration of a pharmaceutical composition comprising a GLP-2, or a variant, an analogue, or derivative of GLP-2 having the ability to bind and activate a GLP-2 receptor such as GLP-2 1-34...

  7. High-level radioactive wastes

    International Nuclear Information System (INIS)

    Grissom, M.C.

    1982-10-01

    This bibliography contains 812 citations on high-level radioactive wastes included in the Department of Energy's Energy Data Base from January 1981 through July 1982. These citations are to research reports, journal articles, books, patents, theses, and conference papers from worldwide sources. Five indexes are provided: Corporate Author, Personal Author, Subject, Contract Number, and Report Number

  8. Very low level radioactive material

    International Nuclear Information System (INIS)

    Schaller, K.H.; Linsley, G.; Elert, M.

    1993-01-01

    Man's environment contains naturally occurring radionuclides and doses from exposures to these radionuclides mostly cannot be avoided. Consequently, almost everything may be considered as very low level radioactive material. In practical terms, management and the selection of different routes for low level material is confined to material which was subject to industrial processing or which is under a system of radiological control. Natural radionuclides with concentrations reaching reporting or notification levels will be discussed below; nevertheless, the main body of this paper will be devoted to material, mainly of artificial origin, which is in the system involving notification, registration and licensing of practices and sources. It includes material managed in the nuclear sector and sources containing artificially produced radionuclides used in hospitals, and in industry. Radioactive materials emit ionising radiations which are harmful to man and his environment. National and international regulations provide the frame for the system of radiation protection. Nevertheless, concentrations, quantities or types of radionuclide may be such, that the material presents a very low hazard, and may therefore be removed from regulatory control, as it would be a waste of time and effort to continue supervision. These materials are said to be exempted from regulatory control. Material exempted in a particular country is no longer distinguishable from ''ordinary'' material and may be moved from country to country. Unfortunately, criteria for exempting radioactive materials differ strongly between countries and free trade. Therefore there is a necessity for an international approach to be developed for exemption levels

  9. FINANCIAL CRISIS FROM THE MACROECONOMIC LEVEL TO THE MICROECONOMIC LEVEL

    OpenAIRE

    SANDA GHEORGHE GABRIEL; ENEA CONSTANTA

    2014-01-01

    The current economic and financial crisis, beyond the problems it generates both at micro and macro level, should determine an adaptation of the mechanisms, institutions and monetary / financial policies to the real economy we are facing. The issue of financial crises is extremely complex, including a series of quantitative and qualitative factors, financial, economic, social, ethical, technological and human, which can intervene and influence the financial operations in a...

  10. Disposal of high level and intermediate level radioactive wastes

    International Nuclear Information System (INIS)

    Flowers, R.H.

    1991-01-01

    The waste products from the nuclear industry are relatively small in volume. Apart from a few minor gaseous and liquid waste streams, containing readily dispersible elements of low radiotoxicity, all these products are processed into stable solid packages for disposal in underground repositories. Because the volumes are small, and because radioactive wastes are latecomers on the industrial scene, a whole new industry with a world-wide technological infrastructure has grown up alongside the nuclear power industry to carry out the waste processing and disposal to very high standards. Some of the technical approaches used, and the Regulatory controls which have been developed, will undoubtedly find application in the future to the management of non-radioactive toxic wastes. The repository site outlined would contain even high-level radioactive wastes and spent fuels being contained without significant radiation dose rates to the public. Water pathway dose rates are likely to be lowest for vitrified high-level wastes with spent PWR fuel and intermediate level wastes being somewhat higher. (author)

  11. Leptin levels in infertile males

    International Nuclear Information System (INIS)

    Jahan, S.; Bibi, R.; Ahmed, S.

    2011-01-01

    Objective: To determine the leptin levels in the serum of normal, sub fertile and infertile men. Study Design: Analytical study. Place and Duration of Study: Department of Animal Sciences Quaid-e-Azam University, Islamabad, National Institute of Health (NIH), Islamabad and Dr. Salma and Kafeel Medical Centre, Islamabad, from April to December 2009. Methodology: Serum leptin levels hormonal concentrations (LH, FSH and testosterone) were determined by EIA in 154 males including 24 (15.58%) fertile, 19 (12.34%) polyzoospermic (PZs), 26 (16.88%) teratozoospermic (TZs), 27 (17.53%) astheno-teratozoospermic (ATZs), 18 (11.69%) oligozoospermic (OZs), 18 (11.69%) oligo-astheno-teratozoospermic (OATZs), 11 (7.14%) obstructive azoospermic (OBST-AZOOs) and 11 (7.14%) non-obstructive azoospermic (NON-OBST-AZOOs). BMI was also determined, divided into groups of greater than 24. Hormonal concentrations were compared by ANOVA and correlation was performed by using Graph pad prism version 5. Results: Significantly high levels of leptin concentrations were found in fertile (p 24 compared to fertile and infertile male patients with BMI 24. Leptin showed a significant positive correlation with LH (p < 0.01) and FSH (p < 0.002) and a significant negative correlation with testosterone (p < 0.001). Conclusion: Abnormal leptin level was significantly associated with fertility problems in males. Providing a link between leptin and reproduction factors contributing in control of testosterone and gonadotropins secretion in many aspects depending on fertility status in male subjects. BMI appears to have significant association with serum leptin levels. (author)

  12. Level gauge using neutron radiation

    International Nuclear Information System (INIS)

    Mathew, P.J.

    1985-01-01

    Apparatus for determining the level of a solid or liquid material in a container comprises: a vertical guide within or alongside the container; a sensor positioned within the guide; means for moving the sensor along the guide; and means for monitoring the position of the sensor. The sensor comprises a source of fast neutrons, a detector for thermal neutrons, and a body of a neutron moderating material in close proximity to the detector. Thermal neutrons produced by fast neutron irradiation of the solid or liquid material, or thermal neutrons produced by irradiation of the neutron-moderating material by fast or epithermal neutrons reflected by the solid or liquid material, are detected when the sensor is positioned at or below the level of the material in the container

  13. Radon levels in Oslo schools

    International Nuclear Information System (INIS)

    Birovlev, A.

    2004-01-01

    Radon measurements using passive CR-39 detectors have been conducted in all schools in Oslo municipality during winter 2003/2004. Results are presented and discussed in the light of qualitative and quantitative factors, some of which are specific for schools as workplaces. Analysis is conducted with respect to factors relating to building construction type, ventilation principle, age of building, building size etc. The influence of ventilation type on radon levels is studied, and problems of investigations based purely on conventional passive radon detectors are noted. Over-estimation of radon concentration by passive detectors and day-night variations of indoor radon levels in buildings with mechanical ventilation systems are discussed. Several guiding principles for planning similar investigations based on above discussions are outlined. (author)

  14. Technology Performance Level Assessment Methodology.

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, Jesse D.; Bull, Diana L; Malins, Robert Joseph; Costello, Ronan Patrick; Aurelien Babarit; Kim Nielsen; Claudio Bittencourt Ferreira; Ben Kennedy; Kathryn Dykes; Jochem Weber

    2017-04-01

    The technology performance level (TPL) assessments can be applied at all technology development stages and associated technology readiness levels (TRLs). Even, and particularly, at low TRLs the TPL assessment is very effective as it, holistically, considers a wide range of WEC attributes that determine the techno-economic performance potential of the WEC farm when fully developed for commercial operation. The TPL assessment also highlights potential showstoppers at the earliest possible stage of the WEC technology development. Hence, the TPL assessment identifies the technology independent “performance requirements.” In order to achieve a successful solution, the entirety of the performance requirements within the TPL must be considered because, in the end, all the stakeholder needs must be achieved. The basis for performing a TPL assessment comes from the information provided in a dedicated format, the Technical Submission Form (TSF). The TSF requests information from the WEC developer that is required to answer the questions posed in the TPL assessment document.

  15. Mission Level Autonomy for USSV

    Science.gov (United States)

    Huntsberger, Terry; Stirb, Robert C.; Brizzolara, Robert

    2011-01-01

    On-water demonstration of a wide range of mission-proven, advanced technologies at TRL 5+ that provide a total integrated, modular approach to effectively address the majority of the key needs for full mission-level autonomous, cross-platform control of USV s. Wide baseline stereo system mounted on the ONR USSV was shown to be an effective sensing modality for tracking of dynamic contacts as a first step to automated retrieval operations. CASPER onboard planner/replanner successfully demonstrated realtime, on-water resource-based analysis for mission-level goal achievement and on-the-fly opportunistic replanning. Full mixed mode autonomy was demonstrated on-water with a seamless transition between operator over-ride and return to current mission plan. Autonomous cooperative operations for fixed asset protection and High Value Unit escort using 2 USVs (AMN1 & 14m RHIB) were demonstrated during Trident Warrior 2010 in JUN 2010

  16. Students as Math Level Designers

    DEFF Research Database (Denmark)

    Jensen, Erik Ottar; Hanghøj, Thorkild; Schoenau-Fog, Henrik

    The short paper presents preliminary findings from a pilot study on how students become motivated through design of learning games in math. The research is carried out in a Danish public school with two classes of 5th graders (N = 42 students). Over the course of two weeks, the students work...... with a design template for a runner game in the Unity 3D game design engine. The students are introduced to the concept of “flow” (Csikszentmihalyi, 1991) as a game design principle and are asked to design levels for a math runner game, which are both engaging as well as a meaningful way of learning math....... In this way, the students are positioned as “math level designers”, which means that they both have to redesign the difficulty of the runner game as well as the difficulty of the mathematical questions and possible answers....

  17. Feature-level domain adaptation

    DEFF Research Database (Denmark)

    Kouw, Wouter M.; Van Der Maaten, Laurens J P; Krijthe, Jesse H.

    2016-01-01

    -level domain adaptation (flda), that models the dependence between the two domains by means of a feature-level transfer model that is trained to describe the transfer from source to target domain. Subsequently, we train a domain-adapted classifier by minimizing the expected loss under the resulting transfer...... modeled via a dropout distribution, which allows the classiffier to adapt to differences in the marginal probability of features in the source and the target domain. Our experiments on several real-world problems show that flda performs on par with state-of-the-art domainadaptation techniques.......Domain adaptation is the supervised learning setting in which the training and test data are sampled from different distributions: training data is sampled from a source domain, whilst test data is sampled from a target domain. This paper proposes and studies an approach, called feature...

  18. Low-level radioactive waste

    International Nuclear Information System (INIS)

    McLaren, L.H.

    1983-03-01

    This bibliography contains information on low-level radioactive waste included in the Department of Energy's Energy Data Base for January through December 1982. The abstracts are grouped by subject category as shown in the table of contents. Entries in the subject index also facilitate access by subject, e.g., Low-Level Radioactive Wastes/Transport. Within each category the arrangement is by report number for reports, followed by nonreports in reverse chronological order. These citations are to research reports, journal articles, books, patents, theses, and conference papers from worldwide sources. Five indexes, each proceded by a brief description, are provided: Corporate Author, Personal Author, Subject, Contract Number, and Report Number. 492 references

  19. Multiple Level Crowding: Crowding at the Object Parts Level and at the Object Configural level.

    Science.gov (United States)

    Kimchi, Ruth; Pirkner, Yossef

    2015-01-01

    In crowding, identification of a peripheral target in the presence of nearby flankers is worse than when the target appears alone. Prevailing theories hold that crowding occurs because of integration or "pooling" of low-level features at a single, relatively early stage of visual processing. Recent studies suggest that crowding can occur also between high-level object representations. The most relevant findings come from studies with faces and may be specific to faces. We examined whether crowding can occur at the object configural level in addition to part-level crowding, using nonface objects. Target (a disconnected square or diamond made of four elements) identification was measured at varying eccentricities. The flankers were similar either to the target parts or to the target configuration. The results showed crowding in both cases: Flankers interfered with target identification such that identification accuracy decreased with an increase in eccentricity, and no interference was observed at the fovea. Crowding by object parts, however, was weaker and had smaller spatial extent than crowding by object configurations; we related this finding to the relationship between crowding and perceptual organization. These results provide strong evidence that crowding occurs not only between object parts but also between configural representations of objects. © The Author(s) 2015.

  20. Level-3 Calorimetric Resolution available for the Level-1 and Level-2 CDF Triggers

    CERN Document Server

    Canepa, A.; Liu, T.; Cortiana, G.; Flanagan, G.; Frisch, H.; Krop, D.; Pilcher, C.; Rusu, V.; Cavaliere, V.; Greco, V.; Giannetti, P.; Piendibene, M.; Sartori, L.; Vidal, Miguel

    2008-01-01

    As the Tevatron luminosity increases sophisticated selections are required to be efficient in selecting rare events among a very huge background. To cope with this problem, CDF has pushed the offline calorimeter algorithm reconstruction resolution up to Level 2 and, when possible, even up to Level 1, increasing efficiency and, at the same time, keeping under control the rates. The CDF Run II Level 2 calorimeter trigger is implemented in hardware and is based on a simple algorithm that was used in Run I. This system has worked well for Run II at low luminosity. As the Tevatron instantaneous luminosity increases, the limitation due to this simple algorithm starts to become clear: some of the most important jet and MET (Missing ET) related triggers have large growth terms in cross section at higher luminosity. In this paper, we present an upgrade of the Level 2 Calorimeter system which makes the calorimeter trigger tower information available directly to a CPU allowing more sophisticated algorithms to be impleme...

  1. System-level musings about system-level science (Invited)

    Science.gov (United States)

    Liu, W.

    2009-12-01

    In teleology, a system has a purpose. In physics, a system has a tendency. For example, a mechanical system has a tendency to lower its potential energy. A thermodynamic system has a tendency to increase its entropy. Therefore, if geospace is seen as a system, what is its tendency? Surprisingly or not, there is no simple answer to this question. Or, to flip the statement, the answer is complex, or complexity. We can understand generally why complexity arises, as the geospace boundary is open to influences from the solar wind and Earth’s atmosphere and components of the system couple to each other in a myriad of ways to make the systemic behavior highly nonlinear. But this still begs the question: What is the system-level approach to geospace science? A reductionist view might assert that as our understanding of a component or subsystem progresses to a certain point, we can couple some together to understand the system on a higher level. However, in practice, a subsystem can almost never been observed in isolation with others. Even if such is possible, there is no guarantee that the subsystem behavior will not change when coupled to others. Hence, there is no guarantee that a subsystem, such as the ring current, has an innate and intrinsic behavior like a hydrogen atom. An absolutist conclusion from this logic can be sobering, as one would have to trace a flash of aurora to the nucleosynthesis in the solar core. The practical answer, however, is more promising; it is a mix of the common sense we call reductionism and awareness that, especially when strongly coupled, subsystems can experience behavioral changes, breakdowns, and catastrophes. If the stock answer to the systemic tendency of geospace is complexity, the objective of the system-level approach to geospace science is to define, measure, and understand this complexity. I will use the example of magnetotail dynamics to illuminate some key points in this talk.

  2. Low level photoneutron detection equipment

    International Nuclear Information System (INIS)

    Ji Changsong; Zhang Yuqin; Li Yuansui

    1991-01-01

    A low level photoneutron detection equipment has been developed. The photoneutrons produced by interaction of 226 Ra gamma quanta and deutron (D) target are detected with n-n discrimination detector made up of 3 He proportional counter array. The D-content information in the target can be obtained from the measured photoneutron counts. The equipment developed is mainly used for nondestructive D-content measurement of D-devices

  3. Level structure of 154Ho

    Science.gov (United States)

    Moon, Chang-Bum; Komatsubara, Tetsuro; Furuno, Kohei

    2013-10-01

    The excited states of the odd-odd 154Ho nucleus have been studied by using in-beam γ-ray spectroscopy with the 141Pr (16O, 3n) 154Ho reaction at Elab=75 MeV. The beam was provided by the 12UD Pelletron accelerator at the University of Tsukuba. In this work, the complicated decay pattern of low energy transitions just above the T1/2=3.10 min isomer have been established. In addition, a number of new states and γ-ray transitions, especially those associated with energetically favored band termination, have been observed for the first time in 154Ho. A negative collective band and its signature partner built on the 11- level are interpreted as being based on the πh11/2⊗νi13/2 configuration. A positive band built on the 10+ level is based on the πh11/2⊗νh9/2 configuration while another positive band built on the 9+ level is being associated with the πh11/2⊗νf7/2 configuration. An energetically favored level Jπ=19- can be interpreted as being attributed to the πh11/2⊗νi13/2 configuration coupled to the 8+ state in neighboring core 152Dy, namely, a four-quasiparticle alignment based on the [πh11/2νi13/2]11-⊗[ν(h9/2f7/2)]8- configuration. Another energetically favored state at Jπ=27- is assigned the six-quasiparticle [π(h11/2)3]27/2-⊗[ν(f7/2h9/2i13/2)]27/2- configuration.

  4. Parathyroid Hormone Levels and Cognition

    Science.gov (United States)

    Burnett, J.; Smith, S.M.; Aung, K.; Dyer, C.

    2009-01-01

    Hyperparathyroidism is a well-recognized cause of impaired cognition due to hypercalcemia. However, recent studies have suggested that perhaps parathyroid hormone itself plays a role in cognition, especially executive dysfunction. The purpose of this study was to explore the relationship of parathyroid hormone levels in a study cohort of elders with impaied cognition. Methods: Sixty community-living adults, 65 years of age and older, reported to Adult Protective Services for self-neglect and 55 controls matched (on age, ethnicity, gender and socio-economic status) consented and participated in this study. The research team conducted in-home comprehensive geriatric assessments which included the Mini-mental state exam (MMSE), the 15-item geriatric depression scale (GDS) , the Wolf-Klein clock test and a comprehensive nutritional panel, which included parathyroid hormone and ionized calcium. Students t tests and linear regression analyses were performed to assess for bivariate associations. Results: Self-neglecters (M = 73.73, sd=48.4) had significantly higher PTH levels compared to controls (M =47.59, sd=28.7; t=3.59, df=98.94, p<.01). There was no significant group difference in ionized calcium levels. Overall, PTH was correlated with the MMSE (r=-.323, p=.001). Individual regression analyses revealed a statistically significant correlation between PTH and MMSE in the self-neglect group (r=-.298, p=.024) and this remained significant after controlling for ionized calcium levels in the regression. No significant associations were revealed in the control group or among any of the other cognitive measures. Conclusion: Parathyroid hormone may be associated with cognitive performance.

  5. Landau levels on a torus

    OpenAIRE

    Enrico OnofriDipartimento di Fisica, Universita` di Parma, and INFN, Gruppo Collegato di Parma, Parma, Italy

    2015-01-01

    Landau levels have represented a very rich field of research, which has gained widespread attention after their application to quantum Hall effect. In a particular gauge, the holomorphic gauge, they give a physical implementation of Bargmann's Hilbert space of entire functions. They have also been recognized as a natural bridge between Feynman's path integral and Geometric Quantization. We discuss here some mathematical subtleties involved in the formulation of the problem when one tries to s...

  6. System level modeling and component level control of fuel cells

    Science.gov (United States)

    Xue, Xingjian

    This dissertation investigates the fuel cell systems and the related technologies in three aspects: (1) system-level dynamic modeling of both PEM fuel cell (PEMFC) and solid oxide fuel cell (SOFC); (2) condition monitoring scheme development of PEM fuel cell system using model-based statistical method; and (3) strategy and algorithm development of precision control with potential application in energy systems. The dissertation first presents a system level dynamic modeling strategy for PEM fuel cells. It is well known that water plays a critical role in PEM fuel cell operations. It makes the membrane function appropriately and improves the durability. The low temperature operating conditions, however, impose modeling difficulties in characterizing the liquid-vapor two phase change phenomenon, which becomes even more complex under dynamic operating conditions. This dissertation proposes an innovative method to characterize this phenomenon, and builds a comprehensive model for PEM fuel cell at the system level. The model features the complete characterization of multi-physics dynamic coupling effects with the inclusion of dynamic phase change. The model is validated using Ballard stack experimental result from open literature. The system behavior and the internal coupling effects are also investigated using this model under various operating conditions. Anode-supported tubular SOFC is also investigated in the dissertation. While the Nernst potential plays a central role in characterizing the electrochemical performance, the traditional Nernst equation may lead to incorrect analysis results under dynamic operating conditions due to the current reverse flow phenomenon. This dissertation presents a systematic study in this regard to incorporate a modified Nernst potential expression and the heat/mass transfer into the analysis. The model is used to investigate the limitations and optimal results of various operating conditions; it can also be utilized to perform the

  7. Effects of birthplace and individual genetic admixture on lung volume and exercise phenotypes of Peruvian Quechua.

    Science.gov (United States)

    Brutsaert, Tom D; Parra, Esteban; Shriver, Mark; Gamboa, Alfredo; Palacios, Jose-Antonio; Rivera, Maria; Rodriguez, Ivette; León-Velarde, Fabiola

    2004-04-01

    Forced vital capacity (FVC) and maximal exercise response were measured in two populations of Peruvian males (age, 18-35 years) at 4,338 m who differed by the environment in which they were born and raised, i.e., high altitude (Cerro de Pasco, Peru, BHA, n = 39) and sea level (Lima, Peru, BSL, n = 32). BSL subjects were transported from sea level to 4,338 m, and were evaluated within 24 hr of exposure to hypobaric hypoxia. Individual admixture level (ADMIX, % Spanish ancestry) was estimated for each subject, using 22 ancestry-informative genetic markers and also by skin reflectance measurement (MEL). Birthplace accounted for the approximately 10% larger FVC (P < 0.001), approximately 15% higher maximal oxygen consumption (VO(2)max, ml.min(-1).kg(-1)) (P < 0.001), and approximately 5% higher arterial oxygen saturation during exercise (SpO(2)) (P < 0.001) of BHA subjects. ADMIX was low in both study groups, averaging 9.5 +/- 2.6% and 2.1 +/- 0.3% in BSL and BHA subjects, respectively. Mean underarm MEL was significantly higher in the BSL group (P < 0.001), despite higher ADMIX. ADMIX was not associated with any study phenotype, but study power was not sufficient to evaluate hypotheses of genetic adaptation via the ADMIX variable. MEL and FVC were positively correlated in the BHA (P = 0.035) but not BSL (P = 0.335) subjects. However, MEL and ADMIX were not correlated across the entire study sample (P = 0.282). In summary, results from this study emphasize the importance of developmental adaptation to high altitude. While the MEL-FVC correlation may reflect genetic adaptation to high altitude, study results suggest that alternate (environmental) explanations be considered. Copyright 2003 Wiley-Liss, Inc.

  8. Serum zinc level in thalassemia

    International Nuclear Information System (INIS)

    Keikhaei, B.; Badavi, M.; Pedram, M.; Zandian, K.

    2010-01-01

    To compare serum zinc level between Thalassemia Major (TM) patients and normal population at Shafa Hospital in South West of Iran. A total of 25 male and 36 female of TM patients were enrolled in this study. Out of 61 patients thirty were treated by deferroxamine (DFO) and 31 were on the combination of DFO and deferiprone (DEF) protocol therapy. Sixty normal subjects of the matching age and gender were recruited as controls. From each patient and control group 2 ml of blood was taken in fasting condition. Cell blood count and serum zinc were carried out for both thalassemia patients and normal subjects. The mean age of patients and control group was 15+- 5 years. Mean serum zinc level was 68.97+- 21.12 mu g/dl, 78.10-28.50 mu g/dl, and 80.16+- 26.54 mu g/dl in the TM with DFO, TM with DFO + DEF combination protocol and control group respectively. There was no significant correlation between patients and control group. However 50 percent of TM with DFO, 38.7 percent of TM with DFO + DEF and 32.8 percent of control group had hypozincemia. Nearly 40 to 50 percent of TM patients and one third of normal subjects are suffering from hypozincemia. This study shows that low level of serum zinc is a health problem in both thalassemia patients and normal population in South West of Iran. (author)

  9. [Alternative nutrition and glutathione levels].

    Science.gov (United States)

    Krajcovicová-Kudlácková, M; Simoncic, R; Béderová, A; Brtková, A; Magálová, T; Barteková, S

    1999-08-30

    Low protein quality and quantity is reported to be a possible risk of alternative nutrition. Pulses contain 18-41% of methionine in relation to reference protein, moreover, its content in cereals is by one half lower. Therefore vegetarians and vegans may have an insufficient intake of sulphur-containing amino acids that may subsequently affect glutathione values (precursors of its synthesis). In groups of adults on an alternative diet--lactoovovegetarians (n = 47) and vegans (n = 44) aged 19-62 years with average duration on a vegetarian or vegan diet of 7.6 and 4.9 years, respectively, glutathione levels (GSH) were measured in erythrocytes (spectrophotometrically), as well as the activity of GSH-dependent enzymes. As nutritional control (n = 42) served an average sample of omnivores selected from a group of 489 examined, apparently healthy subjects of the same age range living in the same region. One to low protein intake (56% of RDA) exclusively of plant origin significantly lower levels of total proteins were observed in vegans with a 16% frequency of hypoproteinaemia (vs 0% in omnivores). In comparison to omnivores a significantly lower glutathione level was found (4.28 +/- 0.12 vs 4.84 +/- 0.14 mumol/g Hb, P vegan diet also in adult age.

  10. Level of Sexual Myths Level in Premature Ejaculation Cases

    Directory of Open Access Journals (Sweden)

    Mehmet Gunes

    2016-06-01

    Full Text Available Objective: The aim of this study is to determine level of belief in sexual myths in the cases of premature ejacula­tion (PE which is the most common sexual dysfunction in men. Methods: This study included 100 cases who applied Di­cle University Faculty of Medicine hospitals meet prema­ture ejaculation criteria of DSM-5 and 70 healthy controls. Sociodemographic data form, Hamilton Depression Rat­ing Scale (HDS, Hamilton Anxiety Rating Scale (HAS, Arizona Sexual Experience Scale (ASES-Men form and Sexual Myths Evaluation Form were applied to partici­pants. Results: In the study, rate of belief in sexual myths in PE cases was found significantly higher than healthy controls. In the PE cases, education time less than 10 years, the presence of comorbid sexual dysfunction were found to be statistically significant factors that increase the level of belief in sexual myths in the PE cases, HDS (p=0.0002, HAS (p=0.0001, ASES (p=0.0004 scores were statisti­cally significantly higher than the control group. In the loss of sexual desire in men with comorbid ASES (p=0.0001, with ED, ASES (p=0.001 and HDS (p=0.040 scores were found statistically significantly higher. Conclusions: Sexual information should be given in the appropriate age by educated person in educational insti­tutions.

  11. Parathyroid Hormone Levels and Cognition

    Science.gov (United States)

    Burnett, J.; Smith, S.M.; Aung, K.; Dyer, C.

    2009-01-01

    Hyperparathyroidism is a well-recognized cause of impaired cognition due to hypercalcemia. However, recent studies have suggested that perhaps parathyroid hormone itself plays a role in cognition, especially executive dysfunction. The purpose of this study was to explore the relationship of parathyroid hormone levels in a study cohort of elders with impaied cognition. Methods: Sixty community-living adults, 65 years of age and older, reported to Adult Protective Services for self-neglect and 55 controls matched (on age, ethnicity, gender and socio-economic status) consented and participated in this study. The research team conducted in-home comprehensive geriatric assessments which included the Mini-mental state exam (MMSE), the 15-item geriatric depression scale (GDS) , the Wolf-Klein clock test and a comprehensive nutritional panel, which included parathyroid hormone and ionized calcium. Students t tests and linear regression analyses were performed to assess for bivariate associations. Results: Self-neglecters (M = 73.73, sd=48.4) had significantly higher PTH levels compared to controls (M =47.59, sd=28.7; t=3.59, df=98.94, plevels. Overall, PTH was correlated with the MMSE (r=-.323, p=.001). Individual regression analyses revealed a statistically significant correlation between PTH and MMSE in the self-neglect group (r=-.298, p=.024) and this remained significant after controlling for ionized calcium levels in the regression. No significant associations were revealed in the control group or among any of the other cognitive measures. Conclusion: Parathyroid hormone may be associated with cognitive performance.

  12. Ground level cosmic ray observations

    Energy Technology Data Exchange (ETDEWEB)

    Stephens, S.A. [Tata Institute of Fundamental Research, Bombay (International Commission on Radiation Units and Measurements); Grimani, C.; Brunetti, M.T.; Codino, A. [Perugia Univ. (Italy)]|[INFN, Perugia (Italy); Papini, P.; Massimo Brancaccio, F.; Piccardi, S. [Florence Univ. (Italy)]|[INFN, Florence (Italy); Basini, G.; Bongiorno, F. [INFN, Laboratori Nazionali di Frascati, Rome (Italy); Golden, R.L. [New Mexico State Univ., Las Cruces, NM (United States). Particle Astrophysics Lab.; Hof, M. [Siegen Univ. (Germany). Fachbereich Physik

    1995-09-01

    Cosmic rays at ground level have been collected using the NMSU/Wizard - MASS2 instrument. The 17-hr observation run was made on September 9. 1991 in Fort Sumner, New Mexico, Usa. Fort Sumner is located at 1270 meters a.s.l., corresponding to an atmospheric depth of about 887 g/cm{sup 2}. The geomagnetic cutoff is 4.5 GV/c. The charge ratio of positive and negative muons and the proton to muon ratio have been determined. These observations will also be compared with data collected at a higher latitude using the same basic apparatus.

  13. Low level radioactive waste disposal

    International Nuclear Information System (INIS)

    Balaz, J.; Chren, O.

    2015-01-01

    The Mochovce National Radwaste Repository is a near surface multi-barrier disposal facility for disposal of processed low and very low level radioactive wastes (radwastes) resulting from the operation and decommissioning of nuclear facilities situated in the territory of the Slovak Republic and from research institutes, laboratories, hospitals and other institutions (institutional RAW) which are in compliance with the acceptance criteria. The basic safety requirement of the Repository is to avoid a radioactive release to the environment during its operation and institutional inspection. This commitment is covered by the protection barrier system. The method of solution designed and implemented at the Repository construction complies with the latest knowledge and practice of the repository developments all over the world and meets requirements for the safe radwaste disposal with minimum environmental consequences. All wastes are solidified and have to meet the acceptance criteria before disposal into the Repository. They are processed and treated at the Bohunice RAW Treatment Centre and Liquid RAW Final Treatment Facility at Mochovce. The disposal facility for low level radwastes consists of two double-rows of reinforced concrete vaults with total capacity 7 200 fibre reinforced concrete containers (FCCs) with RAW. One double-row contains 40 The operation of the Repository was started in year 2001 and after ten years, in 2011 was conducted the periodic assessment of nuclear safety with positive results. Till the end of year 2014 was disposed to the Repository 11 514 m 3 RAW. The analysis of total RAW production from operation and decommissioning of all nuclear installation in SR, which has been carried out in frame of the BIDSF project C9.1, has showed that the total volume estimation of conditioned waste is 108 thousand m 3 of which 45.5 % are low level waste (LLW) and 54,5 % very low level waste (VLLW). On the base of this fact there is the need to build 7

  14. NASA Technology Readiness Level Definitions

    Science.gov (United States)

    Mcnamara, Karen M.

    2012-01-01

    This presentation will cover the basic Technology Readiness Level (TRL) definitions used by the National Aeronautics and Space Administration (NASA) and their specific wording. We will discuss how they are used in the NASA Project Life Cycle and their effectiveness in practice. We'll also discuss the recent efforts by the International Standards Organization (ISO) to develop a broadly acceptable set of TRL definitions for the international space community and some of the issues brought to light. This information will provide input for further discussion of the use of the TRL scale in manufacturing.

  15. Biopower at the molar level

    DEFF Research Database (Denmark)

    Frandsen, Martin; Triantafillou, Peter

    2011-01-01

    Over the last decade or so much social science research on the developments in the life sciences and biomedicine seems to be focusing too narrowly on individualization or, more recently, on geneticization, that is, the ‘molecular’ level. This article argues that current public health policies......, the society and ‘its’ population today serves as a benchmark for a wide array public interventions seeking to make individuals and groups live a life that is longer and of a better quality. This new strategy, which goes well beyond reducing mortality and sickness, seems in principle to be without political...

  16. Radiation Levels around the LHC

    CERN Document Server

    Mala, P; Calviani, M; Nordt, A

    2013-01-01

    This work discuss on the radiation levels measured around the LHC machine during the 2012 operational year. The doses and particle fluences are measured primarily by RadMon detectors – about 300 RadMons are installed around the accelerator – and by thermoluminescent detectors. In addition, BLMs, IG5/PMI ionisation chambers as well as FGCs can be used for corresponding cumulated dose evaluations. The probability of SEE depends directly on the high-energy hadron (HEH) fluence, so this is the main parameter that is calculated based on RadMons counts.

  17. Macroeconomic level of technology transfer

    Directory of Open Access Journals (Sweden)

    Smirnova Nadezhda

    2016-04-01

    Full Text Available World practice of economic management has proved that the best indicator of competitiveness is achieved by that economic system, the economic units of which timely and adequately update the resource and technical base, thus achieving higher financial and economic indicators. Ensuring that sustainable development becomes possible due to the transfer of technological innovations, namely the diffusion from the developer to the customer on both commercial and free of charge basis. The article focuses on functioning of technology transfer at the macro level, namely the creation of its domestic models.

  18. Business plan: LevelUp

    OpenAIRE

    Zhak, Viktor; Sokolov, Ilya

    2013-01-01

    The following thesis was created to enable the writers to produce a business plan for LevelUp Company, gaming café that is going to operate in the center of Helsinki and provide customers playing hours on PC, PS4 and X-box One. The entire business plan describes all aspects concerning marketing analysis, financial data and basic information on the gaming café as location, number of staff, prices open hours etc. The theoretical framework was based on the most reliable marketing and finance...

  19. Level Scheme of 223Fr

    International Nuclear Information System (INIS)

    Gaeta, R.; Gonzalez, J.A.; Gonzalez, L.; Roldan, C.

    1972-01-01

    A study has been made of the decay of 2 27 Ac at levels of 223 F r, means of alpha Spectrometers of Si barrier detector and gamma Spectrometers of Ge(Li). The rotational bands 1/2-(541 ↓ ] , 1/2-(530 ↑ ) and 3/2-(532 ↓ ) have been identified, as well as two octupolar bands associated with the fundamental one. The results obtained indicate that the unified model is applicable in this intermediate zone of the nuclide chart. (Author) 150 refs

  20. Developing State Level Approaches under the State Level Concept

    International Nuclear Information System (INIS)

    Budlong Sylvester, K.; Murphy, C.L.; Boyer, B.; Pilat, J.F.

    2015-01-01

    With the pursuit of the State-Level Concept (SLC), the IAEA has sought to further evolve the international safeguards system in a manner which maintains (or improves) the effectiveness of the system in an environment of expanding demands and limited resources. The IAEA must not remain static and should continuously examine its practices to ensure it can capture opportunities for cost reductions while adapting to, and staying ahead of, emerging proliferation challenges. Contemporary safeguards have been focused on assessing the nuclear programme of the State as a whole, rather than on the basis of individual facilities. Since the IAEA's integrated safeguards program, State-level Approaches (SLAs) have been developed that seek to optimally combine the measures provided for by the Additional Protocol with those of traditional safeguards. This process resulted in facility specific approaches that, while making use of a State's broader conclusion, were nonetheless prescriptive. Designing SLAs on a State-by-State basis would avoid the shortcomings of a one-size-fits-all system. It would also enable the effective use of the Agency's information analysis and State evaluation efforts by linking this analysis to safeguards planning efforts. Acquisition Path Analysis (APA), along with the State Evaluation process, can be used to prioritize paths in a State in terms of their attractiveness for proliferation. While taking advantage of all safeguards relevant information, and tailoring safeguards to individual characteristics of the State, paths of the highest priority in all States will necessarily meet the same standard of coverage. Similarly, lower priority paths will have lower performance targets, thereby promoting nondiscrimination. Such an approach would improve understanding of safeguards implementation under the SLC and the rational for safeguards resource allocation. The potential roles for APA and performance targets in SLA development will be reviewed

  1. Why control blood glucose levels?

    Science.gov (United States)

    Rossini, A A

    1976-03-01

    The controversy as to the relationship between the degree of control of diabetes and the progression of the complications of the disease has not been solved. However, in this review, various studies suggesting a relationship between the metabolic abnormality and the diabetic complications are examined. The disadvantages of the uncontrolled diabetes mellitus can be divided into two major categories-short-term and long-term. The short-term disadvantages of controlled diabetes mellitus include the following: (1) ketoacidosis and hyperosmolar coma; (2) intracellular dehydration; (3) electrolyte imbalance; (4) decreased phagocytosis; (5) immunologic and lymphocyte activity; (6) impairment of wound healing; and (7) abnormality of lipids. The long-term disadvantages of uncontrolled diabetes melitus include the following: (1) nephropathy; (2) neuropathy; (3) retinopathy; (4) cataract formation; (5) effect on perinatal mortality; (6) complications of vascular disease; and (7) the evaluation of various clinical studies suggesting the relationship of elevated blood glucose levels and complications of diabetes mellitus. It is suggested that until the question of control can absolutely be resolved, the recommendation is that the blood glucose levels should be controlled as close to the normal as possible.

  2. Parathyroid Hormone Levels and Cognition

    Science.gov (United States)

    Burnett, J.; Smith, S.M.; Aung, K.; Dyer, C.

    2009-01-01

    Hyperparathyroidism is a well-recognized cause of impaired cognition due to hypercalcemia. However, recent studies have suggested that perhaps parathyroid hormone itself plays a role in cognition, especially executive dysfunction. The purpose of this study was to explore the relationship of parathyroid hormone levels in a study cohort of elders with impaied cognition. Methods: Sixty community-living adults, 65 years of age and older, reported to Adult Protective Services for self-neglect and 55 controls matched (on age, ethnicity, gender and socio-economic status) consented and participated in this study. The research team conducted in-home comprehensive geriatric assessments which included the Mini-mental state exam (MMSE), the 15-item geriatric depression scale (GDS) , the Wolf-Klein clock test and a comprehensive nutritional panel, which included parathyroid hormone and ionized calcium. Students t tests and linear regression analyses were performed to assess for bivariate associations. Results: Self-neglecters (M = 73.73, sd=48.4) had significantly higher PTH levels compared to controls (M =47.59, sd=28.7; t=3.59, df=98.94, pcognitive measures. Conclusion: Parathyroid hormone may be associated with cognitive performance.

  3. Low level radiation: biological effects

    International Nuclear Information System (INIS)

    Loken, M.K.

    1983-01-01

    It is imperative that physicians and scientists using radiations in health care delivery continue to assess the benefits derived, vs. potential risk, to patients and radiation workers being exposed to radiation in its various forms as part of our health delivery system. Insofar as possible we should assure our patients and ourselves that the benefits outweigh the potential hazards involved. Inferences as to the possible biological effects of low level radiation are generally based on extrapolations from those effects observed and measured following acute exposures to considerably higher doses of radiation. Thus, in order to shed light on the question of the possible biological effects of low level radiation, a wide variety of studies have been carried out using cells in culture and various species of plant and animal life. This manuscript makes reference to some of those studies with indications as to how and why the studies were done and the conclusions that might be drawn there from. In addition reference is made to the handling of this information by scientists, by environmentalists, and by the news media. Unfortunately, in many instances the public has been misled by what has been said and/or written. It is hoped that this presentation will provide an understandable and reasonable perspective on the various appropriate uses of radiation in our lives and how such uses do provide significant improvement in our health and in our quality of life

  4. Humidity level In psychrometric processes

    International Nuclear Information System (INIS)

    Mojsovski, Filip

    2008-01-01

    When a thermal engineer needs to control, rather than merely moderate humidity, he must focus on the moisture level as a separate variable - not simply an addition of temperature control. Controlling humidity generally demands a correct psychrometric approach dedicated to that purpose [1].Analysis of the humidity level in psychrometric thermal processes leads to relevant data for theory and practice [2]. This paper presents: (1) the summer climatic curve for the Skopje region, (2) selected results of investigation on farm dryers made outside laboratories. The first purpose of such activity was to examine relations between weather conditions and drying conditions. The estimation of weather condition for the warmest season of the year was realized by a summer climatic curve. In the science of drying, basic drying conditions are temperature, relative humidity and velocity of air, thickness of dried product and dryer construction. The second purpose was to realize correct prediction of drying rates for various psychrometrics drying processes and local products. Test runs with the dryer were carried out over a period of 24 h, using fruits and vegetables as experimental material. Air flow rate through the dryer of 150 m3/h, overall drying rate of 0.04 kg/h and air temperature of 65 oC were reached. Three types of solar dryers, were exploited in the research.

  5. International harmonization of intervention levels

    International Nuclear Information System (INIS)

    Jensen, P.H.

    1994-05-01

    A summary is given on international guidance on intervention levels in circumstances in which remedial actions might be considered in nuclear accident situations. The nature and limitations of international work and their effect on the resulting guidance are discussed. The basic international principles recommended can be expressed in the following compressed form: (1) serious deterministic effects should be avoided by intervention, (2) each protective measure should be justified, i.e. do more good than harm, (3) the level at which the protective measures are to be introduced should be optimized, i.e. do most good, and (4) each protective measure should be optimized separately and independent of all other countermeasures. It is of great importance that decision makers inform the public of all aspects of their decisions in order to make the decisions transparent, especially when the interventions chosen are mainly for political or social reason rather than health protection grounds. Otherwise the public may be misled and the radiological protection community will be mistrusted. 17 refs., 6 tabs

  6. The JET level-1 software

    International Nuclear Information System (INIS)

    McCullen, P.A.; Farthing, J.W.

    1998-01-01

    The complex nature of the JET machine requires a large amount of control parameter preparation, selection and validation before a pulse may be started. Level-1 is defined as the centralized, cross-subsystem control of JET. Before it was introduced over 10 years ago, the Session Leader (SL) who is responsible for specifying the parameter settings for a JET pulse, had virtually no software available to help him except for a simple editor used for the creation of control waveforms. Most of the required parameter settings were calculated by hand and then passed on either verbally or via hand-written forms. These parameters were then set by a large number of people - Local Unit Responsible Officers (LUROs) and CODAS Duty Officers (CDOs) using a wide selection of dedicated software. At this time the Engineer in Charge (EiC) would largely depend on the LUROs to inform him that conditions were ready. He never set control parameters personally and had little or no software available to him to see what many of the settings were. The first implementation of Level-1 software went some way towards improving the task of pulse schedule preparation in that the SL could specify his requirements via a computer interface and store them in a database for later use. At that time the maximum number of parameters that could be handled was 500. (author)

  7. MIPAS level 2 operational analysis

    Directory of Open Access Journals (Sweden)

    P. Raspollini

    2006-01-01

    Full Text Available The MIPAS (Michelson Interferometer for Passive Atmospheric Sounding instrument has been operating on-board the ENVISAT satellite since March 2002. In the first two years, it acquired in a nearly continuous manner high resolution (0.025 cm−1 unapodized emission spectra of the Earth's atmosphere at limb in the middle infrared region. This paper describes the level 2 near real-time (NRT and off-line (OL ESA processors that have been used to derive level 2 geophysical products from the calibrated and geolocated level 1b spectra. The design of the code and the analysis methodology have been driven by the requirements for NRT processing. This paper reviews the performance of the optimized retrieval strategy that has been implemented to achieve these requirements and provides estimated error budgets for the target products: pressure, temperature, O3, H2O, CH4, HNO3, N2O and NO2, in the altitude measurement range from 6 to 68 km. From application to real MIPAS data, it was found that no change was needed in the developed code although an external algorithm was introduced to identify clouds with high opacity and to exclude affected spectra from the analysis. In addition, a number of updates were made to the set-up parameters and to auxiliary data. In particular, a new version of the MIPAS dedicated spectroscopic database was used and, in the OL analysis, the retrieval range was extended to reduce errors due to uncertainties in extrapolation of the profile outside the retrieval range and more stringent convergence criteria were implemented. A statistical analysis on the χ2 values obtained in one year of measurements shows good agreement with the a priori estimate of the forward model errors. On the basis of the first two years of MIPAS measurements the estimates of the forward model and instrument errors are in general found to be conservative with excellent performance demonstrated for frequency calibration. It is noted that the total retrieval

  8. Low-level radwaste solidification

    International Nuclear Information System (INIS)

    Naughton, M.D.; Miller, C.C.; Nelson, R.A.; Tucker, R.F.

    1983-01-01

    This paper reports on a study of ''Advanced Low-Level Radioactive Waste Treatment Systems'' conducted under an EPRI contract. The object of the study is to identify advanced lowlevel radwaste treatment systems that are commercially available or are expected to be in the near future. The current state-ofthe-art in radwaste solidification technology is presented. Related processing technologies, such as the compaction of dry active waste (DAW), containers available for radwaste disposal, and the regulatory aspects of radwaste transportation and solidification, are described. The chemical and physical properties of the currently acceptable solidification agents, as identified in the Barnwell radwaste burial site license, are examined. The solidification agents investigated are hydraulic cements, thermoplastic polymers, and thermosetting polymers. It is concluded that solidification processes are complex and depend not only on the chemical and physical properties of the binder material and the waste, but also on how these materials are mixed

  9. Symmetry and Asymmetry Level Measures

    Directory of Open Access Journals (Sweden)

    Angel Garrido

    2010-04-01

    Full Text Available Usually, Symmetry and Asymmetry are considered as two opposite sides of a coin: an object is either totally symmetric, or totally asymmetric, relative to pattern objects. Intermediate situations of partial symmetry or partial asymmetry are not considered. But this dichotomy on the classification lacks of a necessary and realistic gradation. For this reason, it is convenient to introduce "shade regions", modulating the degree of Symmetry (a fuzzy concept. Here, we will analyze the Asymmetry problem by successive attempts of description and by the introduction of the Asymmetry Level Function, as a new Normal Fuzzy Measure. Our results (both Theorems and Corollaries suppose to be some new and original contributions to such very active and interesting field of research. Previously, we proceed to the analysis of the state of art.

  10. Reactor Emergency Action Level Monitor

    International Nuclear Information System (INIS)

    Touchton, R.A.

    1987-01-01

    The Reactor Emergency Action Level Monitor (REALM) Expert System is designed to provide assistance in the identification of a nuclear power plant emergency situation and the determination of its severity. REALM has been developed to operate in a real-time processing environment. REALM embodies a hybrid architecture utilizing both rule-based reasoning and object-oriented programming techniques borrowed from the Artificial Intelligence discipline of Computer Sciences. The rulebase consists of event-based rules and symptom-based rules. The symptom-based rules go beyond the current EAL structure to address the more problematic scenarios and entail a more symbolic representation of the plant information. The results to date have been encouraging that expert system technology can provide improved emergency decision-making capability in nuclear power plants

  11. Excited levels of Pa-233

    International Nuclear Information System (INIS)

    Vara Cuadrado, J. M.

    1969-01-01

    A study of Pa-233 excited levels from the alpha decay of Np-237 and from beta decay of Th-233 has been performed. The alpha decay spectrum was measured with a semiconductor spectrometer of 18 keV effective resolution (FWHM). Over 13 new lines were identified. The gamma ray spectra of Np-237 and Th-233 were obtained with a Ge-Li detector low and medium range energy lines, and with Si-Li detector for the low energy region. A continuous purification method of Np-237 from its comparatively short-lived daughter Pa-233 was applied. A high number of new lines were identified in both spectra. The gamma-gamma coincidence spectra were obtained with INa(T 1 ) detectors. (Author) 54 refs

  12. Sentence-Level Attachment Prediction

    Science.gov (United States)

    Albakour, M.-Dyaa; Kruschwitz, Udo; Lucas, Simon

    Attachment prediction is the task of automatically identifying email messages that should contain an attachment. This can be useful to tackle the problem of sending out emails but forgetting to include the relevant attachment (something that happens all too often). A common Information Retrieval (IR) approach in analyzing documents such as emails is to treat the entire document as a bag of words. Here we propose a finer-grained analysis to address the problem. We aim at identifying individual sentences within an email that refer to an attachment. If we detect any such sentence, we predict that the email should have an attachment. Using part of the Enron corpus for evaluation we find that our finer-grained approach outperforms previously reported document-level attachment prediction in similar evaluation settings.

  13. Wafer level packaging of MEMS

    International Nuclear Information System (INIS)

    Esashi, Masayoshi

    2008-01-01

    Wafer level packaging plays many important roles for MEMS (micro electro mechanical systems), including cost, yield and reliability. MEMS structures on silicon chips are encapsulated between bonded wafers or by surface micromachining, and electrical interconnections are made from the cavity. Bonding at the interface, such as glass–Si anodic bonding and metal-to-metal bonding, requires electrical interconnection through the lid vias in many cases. On the other hand, lateral electrical interconnections on the surface of the chip are used for bonding with intermediate melting materials, such as low melting point glass and solder. The cavity formed by surface micromachining is made using sacrificial etching, and the openings needed for the sacrificial etching are plugged using deposition sealing methods. Vacuum packaging methods and the structures for electrical feedthrough for the interconnection are discussed in this review. (topical review)

  14. Principles for system level electrochemistry

    Science.gov (United States)

    Thaller, L. H.

    1986-01-01

    The higher power and higher voltage levels anticipated for future space missions have required a careful review of the techniques currently in use to preclude battery problems that are related to the dispersion characteristics of the individual cells. Not only are the out-of-balance problems accentuated in these larger systems, but the thermal management considerations also require a greater degree of accurate design. Newer concepts which employ active cooling techniques are being developed which permit higher rates of discharge and tighter packing densities for the electrochemical components. This paper will put forward six semi-independent principles relating to battery systems. These principles will progressively address cell, battery and finally system related aspects of large electrochemical storage systems.

  15. Ionizing radiation: levels and effects. Volume I. Levels

    International Nuclear Information System (INIS)

    1972-01-01

    This is the sixth substantive report of the United Nations Scientific Committee on the Effects of Atomic Radiation to the General Assembly. It reviews the levels of radiation received from all sources to which man is exposed and, among the effects of ionizing radiation, it considers the genetic effects, the effects on the immune response and the induction of malignancies in animals and man. These are not the only effects of ionizing radiation. The acute consequences of massive amounts of radiation that may be received accidentally or during nuclear warfare are not reviewed here (the short discussion of this subject in the 1962 report is still largely valid, at least as an introduction), nor are the effects on the nervous system and the induction of chromosome anomalies in somatic cells, which were both considered by the Committee in its 1969 report. Unlike previous reports of the Committee, the present report is submitted to the General Assembly without the technical annexes in which the evidence considered by the Committee is discussed in detail and in which the bases for the Committee's conclusions, which are stated in the report, are fully documented. However, the annexes are being made available at the same time as the report in a separate publication, issued in two volumes and the Committee wishes to draw the attention of the General Assembly to the fact that the separation of the report from the annexes is for convenience only and that major importance attaches to the scientific evidence given in the annexes.

  16. Colloids related to low level and intermediate level waste

    International Nuclear Information System (INIS)

    Ramsay, J.D.F.; Russell, P.J.; Avery, R.G.

    1991-03-01

    A comprehensive investigation has been undertaken to improve the understanding of the potential role of colloids in the context of disposal and storage of low and intermediate level waste immobilised in cement. Several topics have been investigated using a wide range of advanced physico-chemical and analytical techniques. These include: (a) the study of formation and characteristics of colloids in cement leachates, (b) the effects of the near-field aqueous chemistry on the characteristics of colloids in repository environments, (c) colloid sorption behaviour, (d) interactions of near-field materials with leachates, and (e) preliminary assessment of colloid migration behaviour. It has been shown that the generation of colloids in cement leachates can arise from a process of nucleation and growth leading to an amorphous phase which is predominantly calcium silicate hydrate. Such colloidal material has a capacity for association with polyvalent rare earths and actinides and these may be significant in the source term and processes involving radionuclide retention in the near field. It has also been shown that the near-field aqueous chemistry (pH, Ca 2+ concentration) has a marked effect on colloid behaviour (deposition and stability). A mechanistic approach to predict colloid sorption affinity has been developed which highlights the importance of colloid characteristics and the nature of the ionic species. (author)

  17. Nuclear power: levels of safety

    International Nuclear Information System (INIS)

    Lidsky, L.M.

    1988-01-01

    The rise and fall of the nuclear power industry in the United States is a well-documented story with enough socio-technological conflict to fill dozens of scholarly, and not so scholarly, books. Whatever the reasons for the situation we are now in, and no matter how we apportion the blame, the ultimate choice of whether to use nuclear power in this country is made by the utilities and by the public. Their choices are, finally, based on some form of risk-benefit analysis. Such analysis is done in well-documented and apparently logical form by the utilities and in a rather more inchoate but not necessarily less accurate form by the public. Nuclear power has failed in the United States because both the real and perceived risks outweigh the potential benefits. The national decision not to rely upon nuclear power in its present form is not an irrational one. A wide ranging public balancing of risk and benefit requires a classification of risk which is clear and believable for the public to be able to assess the risks associated with given technological structures. The qualitative four-level safety ladder provides such a framework. Nuclear reactors have been designed which fit clearly and demonstrably into each of the possible qualitative safety levels. Surprisingly, it appears that safer may also mean cheaper. The intellectual and technical prerequisites are in hand for an important national decision. Deployment of a qualitatively different second generation of nuclear reactors can have important benefits for the United States. Surprisingly, it may well be the nuclear establishment itself, with enormous investments of money and pride in the existing nuclear systems, that rejects second generation reactors. It may be that we will not have a second generation of reactors until the first generation of nuclear engineers and nuclear power advocates has retired

  18. Involve women at many levels.

    Science.gov (United States)

    Huq, N

    1995-03-01

    The organizers of the 1994 International Conference on Population and Development (ICPD) realize that slowing the rate of population growth requires the involvement of women at many levels. The planning processes in countries leading up to the ICPD were required to include women, and funds became available for women to attend regional and international preparatory meetings. National delegations at the ICPD also included many more women than the previous two world population conferences in 1974 and 1984. Space was also provided at the ICPD for the involvement of nongovernmental organizations. Naripokkho, a women's advocacy organization in Bangladesh, was therefore able to communicate its message at the conference. In preparation, the organization held consultations and workshops with grassroots women in thirteen regions of Bangladesh. Approximately one third of the women in the workshops had more children than they desired, many felt that they had to have at least two sons, poor services led women to discontinue contraceptive use, and very few women reported that a lack of access to contraception or method failure was responsible for their large families. It was also determined during the preparatory phase that environmental damage cannot be linked to population in a simplistic manner, history, politics, geography, business, and economics play important roles. Once at the ICPD, Naripokkho they negotiated and lobbied the governments to influence the ICPD program of action. Gains for women were made in both language and substance. Indeed, the program of action is the most progressive population document ever issued by a mainstream institution, gives women's goals new legitimacy, and is a powerful tool for groups working at the grassroots level. Governments must now be held to their commitments made in the program.

  19. Low-level Radioactive waste Management

    International Nuclear Information System (INIS)

    1991-01-01

    This meeting describes low-level radioactive waste management problems and contains 8 papers: 1 Low-level radioactive waste management: exemption concept and criteria used by international organizations. 2 Low-level radioactive waste management: french and foreign regulations 3 Low-level radioactive waste management in EDF nuclear power plants (FRANCE) 4 Low-level radioactive waste management in COGEMA (FRANCE) 5 Importance of low-level radioactive wastes in dismantling strategy in CEA (FRANCE) 6 Low-level radioactive waste management in hospitals 7 Low-level radioactive waste disposal: radiation protection laws 8 Methods of low-level radioactive materials measurements during reactor dismantling or nuclear facilities demolition (FRANCE)

  20. Removing high-level contaminants

    International Nuclear Information System (INIS)

    Wallace, Paula

    2013-01-01

    Full text: Using biomimicry, an Australian cleantech innovation making inroads intoChinas's industrial sector offers multiple benefits to miners and processors in Australia. Stephen Shelley, the executive chairman of Creative Water Technology (CWT), was on hand at a recent trade show to explain how his Melbourne company has developed world-class techniques in zero liquid discharge and fractional crystallization of minerals to apply to a wide range of water treatment and recycling applications. “Most existing technologies operate with high energy distillation, filters or biological processing. CWT's appliance uses a low temperature, thermal distillation process known as adiabatic recovery to desalinate, dewater and/or recycle highly saline and highly contaminated waste water,” said Shelley. The technology has been specifically designed to handle the high levels of contaminant that alternative technologies struggle to process, with proven water quality results for feed water samples with TDS levels over 300,000ppm converted to clean water with less than 20ppm. Comparatively, reverse osmosis struggles to process contaminant levels over 70,000ppm effectively. “CWT is able to reclaim up to 97% clean usable water and up to 100% of the contaminants contained in the feed water,” said Shelley, adding that soluble and insoluble contaminants are separately extracted and dried for sale or re-use. In industrial applications CWT has successfully processed feed water with contaminant levels over 650,000 mg/1- without the use of chemicals. “The technology would be suitable for companies in oil exploration and production, mining, smelting, biofuels, textiles and the agricultural and food production sectors,” said Shelley. When compared to a conventional desalination plant, the CWT system is able to capture the value in the brine that most plants discard, not only from the salt but the additional water it contains. “If you recover those two commodities... then you