WorldWideScience

Sample records for biological laboratory project

  1. Biotechnology by Design: An Introductory Level, Project-Based, Synthetic Biology Laboratory Program for Undergraduate Students†

    OpenAIRE

    Beach, Dale L.; Alvarez, Consuelo J.

    2015-01-01

    Synthetic biology offers an ideal opportunity to promote undergraduate laboratory courses with research-style projects, immersing students in an inquiry-based program that enhances the experience of the scientific process. We designed a semester-long, project-based laboratory curriculum using synthetic biology principles to develop a novel sensory device. Students develop subject matter knowledge of molecular genetics and practical skills relevant to molecular biology, recombinant DNA techniq...

  2. Multiweek Cell Culture Project for Use in Upper-Level Biology Laboratories

    Science.gov (United States)

    Marion, Rebecca E.; Gardner, Grant E.; Parks, Lisa D.

    2012-01-01

    This article describes a laboratory protocol for a multiweek project piloted in a new upper-level biology laboratory (BIO 426) using cell culture techniques. Human embryonic kidney-293 cells were used, and several culture media and supplements were identified for students to design their own experiments. Treatments included amino acids, EGF,…

  3. Biotechnology by Design: An Introductory Level, Project-Based, Synthetic Biology Laboratory Program for Undergraduate Students.

    Science.gov (United States)

    Beach, Dale L; Alvarez, Consuelo J

    2015-12-01

    Synthetic biology offers an ideal opportunity to promote undergraduate laboratory courses with research-style projects, immersing students in an inquiry-based program that enhances the experience of the scientific process. We designed a semester-long, project-based laboratory curriculum using synthetic biology principles to develop a novel sensory device. Students develop subject matter knowledge of molecular genetics and practical skills relevant to molecular biology, recombinant DNA techniques, and information literacy. During the spring semesters of 2014 and 2015, the Synthetic Biology Laboratory Project was delivered to sophomore genetics courses. Using a cloning strategy based on standardized BioBrick genetic "parts," students construct a "reporter plasmid" expressing a reporter gene (GFP) controlled by a hybrid promoter regulated by the lac-repressor protein (lacI). In combination with a "sensor plasmid," the production of the reporter phenotype is inhibited in the presence of a target environmental agent, arabinose. When arabinose is absent, constitutive GFP expression makes cells glow green. But the presence of arabinose activates a second promoter (pBAD) to produce a lac-repressor protein that will inhibit GFP production. Student learning was assessed relative to five learning objectives, using a student survey administered at the beginning (pre-survey) and end (post-survey) of the course, and an additional 15 open-ended questions from five graded Progress Report assignments collected throughout the course. Students demonstrated significant learning gains (p Biology Laboratory Project enhanced their understanding of molecular genetics. The laboratory project is highly adaptable for both introductory and advanced courses.

  4. Connecting biology and organic chemistry introductory laboratory courses through a collaborative research project.

    Science.gov (United States)

    Boltax, Ariana L; Armanious, Stephanie; Kosinski-Collins, Melissa S; Pontrello, Jason K

    2015-01-01

    Modern research often requires collaboration of experts in fields, such as math, chemistry, biology, physics, and computer science to develop unique solutions to common problems. Traditional introductory undergraduate laboratory curricula in the sciences often do not emphasize connections possible between the various disciplines. We designed an interdisciplinary, medically relevant, project intended to help students see connections between chemistry and biology. Second term organic chemistry laboratory students designed and synthesized potential polymer inhibitors or inducers of polyglutamine protein aggregation. The use of novel target compounds added the uncertainty of scientific research to the project. Biology laboratory students then tested the novel potential pharmaceuticals in Huntington's disease model assays, using in vitro polyglutamine peptide aggregation and in vivo lethality studies in Drosophila. Students read articles from the primary literature describing the system from both chemical and biological perspectives. Assessment revealed that students emerged from both courses with a deeper understanding of the interdisciplinary nature of biology and chemistry and a heightened interest in basic research. The design of this collaborative project for introductory biology and organic chemistry labs demonstrated how the local interests and expertise at a university can be drawn from to create an effective way to integrate these introductory courses. Rather than simply presenting a series of experiments to be replicated, we hope that our efforts will inspire other scientists to think about how some aspect of authentic work can be brought into their own courses, and we also welcome additional collaborations to extend the scope of the scientific exploration. © 2015 The International Union of Biochemistry and Molecular Biology.

  5. Multiweek cell culture project for use in upper-level biology laboratories.

    Science.gov (United States)

    Marion, Rebecca E; Gardner, Grant E; Parks, Lisa D

    2012-06-01

    This article describes a laboratory protocol for a multiweek project piloted in a new upper-level biology laboratory (BIO 426) using cell culture techniques. Human embryonic kidney-293 cells were used, and several culture media and supplements were identified for students to design their own experiments. Treatments included amino acids, EGF, caffeine, epinephrine, heavy metals, and FBS. Students researched primary literature to determine their experimental variables, made their own solutions, and treated their cells over a period of 2 wk. Before this, a sterile technique laboratory was developed to teach students how to work with the cells and minimize contamination. Students designed their experiments, mixed their solutions, seeded their cells, and treated them with their control and experimental media. Students had the choice of manipulating a number of variables, including incubation times, exposure to treatment media, and temperature. At the end of the experiment, students observed the effects of their treatment, harvested and dyed their cells, counted relative cell numbers in control and treatment flasks, and determined the ratio of living to dead cells using a hemocytometer. At the conclusion of the experiment, students presented their findings in a poster presentation. This laboratory can be expanded or adapted to include additional cell lines and treatments. The ability to design and implement their own experiments has been shown to increase student engagement in the biology-related laboratory activities as well as develop the critical thinking skills needed for independent research.

  6. Implementation of a Project-Based Molecular Biology Laboratory Emphasizing Protein Structure-Function Relationships in a Large Introductory Biology Laboratory Course

    Science.gov (United States)

    Treacy, Daniel J.; Sankaran, Saumya M.; Gordon-Messer, Susannah; Saly, Danielle; Miller, Rebecca; Isaac, R. Stefan; Kosinski-Collins, Melissa S.

    2011-01-01

    In introductory laboratory courses, many universities are turning from traditional laboratories with predictable outcomes to inquiry-inspired, project-based laboratory curricula. In these labs, students are allowed to design at least some portion of their own experiment and interpret new, undiscovered data. We have redesigned the introductory…

  7. Laboratory of Biological Modeling

    Data.gov (United States)

    Federal Laboratory Consortium — The Laboratory of Biological Modeling is defined by both its methodologies and its areas of application. We use mathematical modeling in many forms and apply it to a...

  8. Connecting Biology and Organic Chemistry Introductory Laboratory Courses through a Collaborative Research Project

    Science.gov (United States)

    Boltax, Ariana L.; Armanious, Stephanie; Kosinski-Collins, Melissa S.; Pontrello, Jason K.

    2015-01-01

    Modern research often requires collaboration of experts in fields, such as math, chemistry, biology, physics, and computer science to develop unique solutions to common problems. Traditional introductory undergraduate laboratory curricula in the sciences often do not emphasize connections possible between the various disciplines. We designed an…

  9. Laboratory and project based learning in the compulsory course Biological Chemistry enhancing collaboration and technical communication between groups

    DEFF Research Database (Denmark)

    Agersø, Yvonne; Bysted, Anette; Jensen, Lars Bogø

    2011-01-01

    The aim of this paper was to describe how changes of laboratory training and project based learning were implemented in order to train the students in making a study design, basic laboratory skills, handling of data, technical communication, collaboration and presentation....

  10. Environmental regulation of plant gene expression: an RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course.

    Science.gov (United States)

    Eickelberg, Garrett J; Fisher, Alison J

    2013-01-01

    We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current "gold standard" for gene expression analysis, including detailed data analysis using the common 2-ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. Copyright © 2013 Wiley Periodicals, Inc.

  11. European Molecular Biology Laboratory

    CERN Multimedia

    1973-01-01

    On 10 May an Agreement was signed at CERN setting up a new European Laboratory. It will be concerned with research in molecularbiology and will be located at Heidelberg in the Federal Republic of Germany.

  12. pGLO Mutagenesis: A Laboratory Procedure in Molecular Biology for Biology Students

    Science.gov (United States)

    Bassiri, Eby A.

    2011-01-01

    A five-session laboratory project was designed to familiarize or increase the laboratory proficiency of biology students and others with techniques and instruments commonly used in molecular biology research laboratories and industries. In this project, the EZ-Tn5 transposon is used to generate and screen a large number of cells transformed with…

  13. [Theme: Achieving Quality Laboratory Projects.[.

    Science.gov (United States)

    Shinn, Glen C.; And Others

    1983-01-01

    The theme articles present strategies for achieving quality laboratory projects in vocational agriculture. They describe fundamentals of the construction of quality projects and stress the importance of quality instruction. (JOW)

  14. Space Synthetic Biology Project

    Science.gov (United States)

    Howard, David; Roman, Monsi; Mansell, James (Matt)

    2015-01-01

    Synthetic biology is an effort to make genetic engineering more useful by standardizing sections of genetic code. By standardizing genetic components, biological engineering will become much more similar to traditional fields of engineering, in which well-defined components and subsystems are readily available in markets. Specifications of the behavior of those components and subsystems can be used to model a system which incorporates them. Then, the behavior of the novel system can be simulated and optimized. Finally, the components and subsystems can be purchased and assembled to create the optimized system, which most often will exhibit behavior similar to that indicated by the model. The Space Synthetic Biology project began in 2012 as a multi-Center effort. The purpose of this project was to harness Synthetic Biology principals to enable NASA's missions. A central target for application was to Environmental Control & Life Support (ECLS). Engineers from NASA Marshall Space Flight Center's (MSFC's) ECLS Systems Development Branch (ES62) were brought into the project to contribute expertise in operational ECLS systems. Project lead scientists chose to pursue the development of bioelectrochemical technologies to spacecraft life support. Therefore, the ECLS element of the project became essentially an effort to develop a bioelectrochemical ECLS subsystem. Bioelectrochemical systems exploit the ability of many microorganisms to drive their metabolisms by direct or indirect utilization of electrical potential gradients. Whereas many microorganisms are capable of deriving the energy required for the processes of interest (such as carbon dioxide (CO2) fixation) from sunlight, it is believed that subsystems utilizing electrotrophs will exhibit smaller mass, volume, and power requirements than those that derive their energy from sunlight. In the first 2 years of the project, MSFC personnel conducted modeling, simulation, and conceptual design efforts to assist the

  15. Laboratory of Cell and Molecular Biology

    Data.gov (United States)

    Federal Laboratory Consortium — The Laboratory of Cell and Molecular Biology investigates the organization, compartmentalization, and biochemistry of eukaryotic cells and the pathology associated...

  16. Biological restoration of major transportation facilities domestic demonstration and application project (DDAP): technology development at Sandia National Laboratories.

    Energy Technology Data Exchange (ETDEWEB)

    Ramsey, James L., Jr. (.,; .); Melton, Brad; Finley, Patrick; Brockman, John; Peyton, Chad E.; Tucker, Mark David; Einfeld, Wayne; Griffith, Richard O.; Brown, Gary Stephen; Lucero, Daniel A.; Betty, Rita G.; McKenna, Sean Andrew; Knowlton, Robert G.; Ho, Pauline

    2006-06-01

    The Bio-Restoration of Major Transportation Facilities Domestic Demonstration and Application Program (DDAP) is a designed to accelerate the restoration of transportation nodes following an attack with a biological warfare agent. This report documents the technology development work done at SNL for this DDAP, which include development of the BROOM tool, an investigation of surface sample collection efficiency, and a flow cytometry study of chlorine dioxide effects on Bacillus anthracis spore viability.

  17. Environmental Regulation of Plant Gene Expression: An Rt-qPCR Laboratory Project for an Upper-Level Undergraduate Biochemistry or Molecular Biology Course

    Science.gov (United States)

    Eickelberg, Garrett J.; Fisher, Alison J.

    2013-01-01

    We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the "FLOWERING LOCUS C" gene, a key regulator of floral timing in "Arabidopsis thaliana" plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate…

  18. Sample collections from healthy volunteers for biological variation estimates' update: a new project undertaken by the Working Group on Biological Variation established by the European Federation of Clinical Chemistry and Laboratory Medicine.

    Science.gov (United States)

    Carobene, Anna; Strollo, Marta; Jonker, Niels; Barla, Gerhard; Bartlett, William A; Sandberg, Sverre; Sylte, Marit Sverresdotter; Røraas, Thomas; Sølvik, Una Ørvim; Fernandez-Calle, Pilar; Díaz-Garzón, Jorge; Tosato, Francesca; Plebani, Mario; Coşkun, Abdurrahman; Serteser, Mustafa; Unsal, Ibrahim; Ceriotti, Ferruccio

    2016-10-01

    Biological variation (BV) data have many fundamental applications in laboratory medicine. At the 1st Strategic Conference of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) the reliability and limitations of current BV data were discussed. The EFLM Working Group on Biological Variation is working to increase the quality of BV data by developing a European project to establish a biobank of samples from healthy subjects to be used to produce high quality BV data. The project involved six European laboratories (Milan, Italy; Bergen, Norway; Madrid, Spain; Padua, Italy; Istanbul, Turkey; Assen, The Netherlands). Blood samples were collected from 97 volunteers (44 men, aged 20-60 years; 43 women, aged 20-50 years; 10 women, aged 55-69 years). Initial subject inclusion required that participants completed an enrolment questionnaire to verify their health status. The volunteers provided blood specimens once per week for 10 weeks. A short questionnaire was completed and some laboratory tests were performed at each sampling consisting of blood collected under controlled conditions to provide serum, K2EDTA-plasma and citrated-plasma samples. Samples from six out of the 97 enroled subjects were discarded as a consequence of abnormal laboratory measurements. A biobank of 18,000 aliquots was established consisting of 120 aliquots of serum, 40 of EDTA-plasma, and 40 of citrated-plasma from each subject. The samples were stored at -80 °C. A biobank of well-characterised samples collected under controlled conditions has been established delivering a European resource to enable production of contemporary BV data.

  19. Plant Biology Science Projects.

    Science.gov (United States)

    Hershey, David R.

    This book contains science projects about seed plants that deal with plant physiology, plant ecology, and plant agriculture. Each of the projects includes a step-by-step experiment followed by suggestions for further investigations. Chapters include: (1) "Bean Seed Imbibition"; (2) "Germination Percentages of Different Types of Seeds"; (3)…

  20. Mostly Plants. Individualized Biology Activities on: I. Investigating Bread Mold; II. Transpiration; III. Botany Project; IV. Collecting/Preserving/Identifying Leaves; [and] V. Student Science Laboratory Write-Ups.

    Science.gov (United States)

    Gibson, Paul R.

    Individualized biology activities for secondary students are presented in this teaching guide. The guide is divided into five sections: (1) investigating bread mold; (2) investigating transpiration; (3) completing a botany project; (4) collecting, preserving, and identifying leaves; and (5) writing up science laboratory investigations. The…

  1. Processing laboratory of radio sterilized biological tissues

    International Nuclear Information System (INIS)

    Aguirre H, Paulina; Zarate S, Herman; Silva R, Samy; Hitschfeld, Mario

    2005-01-01

    The nuclear development applications have also reached those areas related to health. The risk of getting contagious illnesses through applying biological tissues has been one of the paramount worries to be solved since infectious illnesses might be provoked by virus, fungis or bacterias coming from donors or whether they have been introduced by means of intermediate stages before the use of these tissues. Therefore it has been concluded that the tissue allografts must be sterilized. The sterilization of medical products has been one of the main applications of the ionizing radiations and that it is why the International Organization of Atomic Energy began in the 70s promoting works related to the biological tissue sterilization and pharmaceutical products. The development of different tissue preservation methods has made possible the creation of tissue banks in different countries, to deal with long-term preservation. In our country, a project was launched in 1998, 'Establishment of a Tissue Bank in Latino america', this project was supported by the OIEA through the project INT/ 6/ 049, and was the starting of the actual Processing Laboratory of Radioesterilized Biological Tissues (LPTR), leaded by the Chilean Nuclear Energy Commission (CCHEN). This first organization is part of a number of entities compounding the Tissue Bank in Chile, organizations such as the Transplantation Promotion Corporation hospitals and the LPTR. The working system is carried out by means of the interaction between the hospitals and the laboratory. The medical professionals perform the procuring of tissues in the hospitals, then send them to the LPTR where they are processed and sterilized with ionizing radiation. The cycle ends up with the tissues return released to the hospitals, where they are used, and then the result information is sent to the LPTR as a form of feedback. Up to now, human skin has been processed (64 donors), amniotic membranes (35 donors) and pig skin (175 portions

  2. Virtual Laboratories in Chemistry, Biochemistry, & Molecular Biology

    DEFF Research Database (Denmark)

    May, Michael; Achiam, Marianne

    2013-01-01

    Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495....

  3. Virtual Laboratories in Chemistry, Biochemistry, & Molecular Biology

    DEFF Research Database (Denmark)

    May, Michael; Achiam, Marianne

    2013-01-01

    Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495.......Report (state-of-the-art review) from a research and development project on virtual laboratories supported by Markedmodningsfonden (tidl. "Fornyelsesfonden")(2012-2014). http://markedsmodningsfonden.dk/projekt/0/34/495....

  4. Laboratory Safety in the Biology Lab.

    Science.gov (United States)

    Ritch, Donna; Rank, Jane

    2001-01-01

    Reports on a research project to determine if students possess and comprehend basic safety knowledge. Shows a significant increase in the amount of safety knowledge gained when students are exposed to various topics in laboratory safety and are held accountable for learning the information as required in a laboratory safety course. (Author/MM)

  5. Project management: importance for diagnostic laboratories.

    Science.gov (United States)

    Croxatto, A; Greub, G

    2017-07-01

    The need for diagnostic laboratories to improve both quality and productivity alongside personnel shortages incite laboratory managers to constantly optimize laboratory workflows, organization, and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches to maximize the opportunities for successful completion of the project. This review aims at presenting a general overview of project management with an emphasis on selected critical aspects. Conventional project management tools and models, such as HERMES, described in the literature, associated personal experience, and educational courses on management have been used to illustrate this review. This review presents general guidelines of project management and highlights their importance for microbiology diagnostic laboratories. As an example, some critical aspects of project management will be illustrated with a project of automation, as experienced at the laboratories of bacteriology and hygiene of the University Hospital of Lausanne. It is important to define clearly beforehand the objective of a project, its perimeter, its costs, and its time frame including precise duration estimates of each step. Then, a project management plan including explanations and descriptions on how to manage, execute, and control the project is necessary to continuously monitor the progression of a project to achieve its defined goals. Moreover, a thorough risk analysis with contingency and mitigation measures should be performed at each phase of a project to minimize the impact of project failures. The increasing complexities of modern laboratories mean clinical microbiologists must use several management tools including project and change management to improve the outcome of major projects and activities. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  6. Certificate of Waiver Laboratory Project

    Data.gov (United States)

    U.S. Department of Health & Human Services — CLIA requires all laboratories that examine materials derived from the human body for diagnosis, prevention, or treatment purposes to be certified by the Secretary...

  7. Egg Yolk Lecithin: A Biochemical Laboratory Project

    Science.gov (United States)

    White, Bernard J.; And Others

    1974-01-01

    Describes an undergraduate laboratory project involving lecithin which integrates two general aspects of lipid methodology: chromatographic techniques and use of enzymes specificity to obtain structural information. (Author/SLH)

  8. AFSC Laboratory Management Information Requirements Project

    National Research Council Canada - National Science Library

    1982-01-01

    This document was developed under the auspices of the Laboratory IRM (LIRM) Management Working Group in response to AFSC Program Directive 0008-81-1, Management Information Requirement Project (23 February 1981...

  9. Myoglobin structure and function: A multiweek biochemistry laboratory project.

    Science.gov (United States)

    Silverstein, Todd P; Kirk, Sarah R; Meyer, Scott C; Holman, Karen L McFarlane

    2015-01-01

    We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure, students work with computer modeling and visualization of myoglobin and its homologues, after which they spectroscopically characterize its thermal denaturation. Students also study protein function (ligand binding equilibrium) and are instructed on topics in data analysis (calibration curves, nonlinear vs. linear regression). This upper division biochemistry laboratory project is a challenging and rewarding one that not only exposes students to a wide variety of important biochemical laboratory techniques but also ties those techniques together to work with a single readily available and easily characterized protein, myoglobin. © 2015 International Union of Biochemistry and Molecular Biology.

  10. Investigating Coccolithophorid Biology in the Sedimentary Laboratory

    Science.gov (United States)

    McClelland, H. L. O.; Barbarin, N.; Beaufort, L.; Hermoso, M.; Rickaby, R. E. M.

    2014-12-01

    Coccolithophores are the ocean's dominant calcifying phytoplankton; they play an important, but poorly understood, role in long-term biogeochemical climatic feedbacks. Calcite producing marine organisms are likely to calcify less in a future world where higher carbon dioxide concentrations will lead to ocean acidification (OA), but coccolithophores may be the exception. In coccolithophores calcification occurs in an intracellular vesicle, where the site of calcite precipitation is buffered from the external environment and is subject to a uniquely high degree of biological control. Culture manipulation experiments mimicking the effects of OA in the laboratory have yielded empirical evidence for phenotypic plasticity, competition and evolutionary adaptation in asexual populations. However, the extent to which these results are representative of natural populations, and of the response over timescales of greater than a few hundred generations, is unclear. Here we describe a new sediment-based proxy for the PIC:POC (particulate inorganic to particulate organic carbon ratio) of coccolithophore biomass, which is equivalent to the fractional energy contribution to calcification at constant pH, and a biologically meaningful measure of the organism's tendency to calcify. Employing the geological record as a laboratory, we apply this proxy to sedimentary material from the southern Pacific Ocean to investigate the integrated response of real ancient coccolithophore populations to environmental change over many thousands of years. Our results provide a new perspective on phenotypic change in real populations of coccolithophorid algae over long timescales.

  11. "Mini-Array" Transcriptional Analysis of the "Listeria Monocytogenes" Lecithinase Operon as a Class Project: A Student Investigative Molecular Biology Laboratory Experience

    Science.gov (United States)

    Christensen, Douglas; Jovic, Marko

    2006-01-01

    This report describes a molecular biotechnology-based laboratory curriculum developed to accompany an undergraduate genetics course. During the course of a semester, students researched the pathogen, developed a research question, designed experiments, and performed transcriptional analysis of a set of genes that confer virulence to the food-borne…

  12. INNOVATIONS IN EQUIPMENT AND TECHNIQUES FOR THE BIOLOGY TEACHING LABORATORY.

    Science.gov (United States)

    BARTHELEMY, RICHARD E.; AND OTHERS

    LABORATORY TECHNIQUES AND EQUIPMENT APPROPRIATE FOR TEACHING BIOLOGICAL SCIENCE CURRICULUM STUDY BIOLOGY ARE EMPHASIZED. MAJOR CATEGORIES INCLUDE (1) LABORATORY FACILITIES, (2) EQUIPMENT AND TECHNIQUES FOR CULTURE OF MICRO-ORGANISMS, (3) LABORATORY ANIMALS AND THEIR HOUSING, (4) TECHNIQUES FOR STUDYING PLANT GROWTH, (5) TECHNIQUES FOR STUDYING…

  13. Structural biology at York Structural Biology Laboratory; laboratory information management systems for structural genomics

    Czech Academy of Sciences Publication Activity Database

    Dohnálek, Jan

    2005-01-01

    Roč. 12, č. 1 (2005), s. 3 ISSN 1211-5894. [Meeting of Structural Biologists /4./. 10.03.2005-12.03.2005, Nové Hrady] R&D Projects: GA MŠk(CZ) 1K05008 Keywords : structural biology * LIMS * structural genomics Subject RIV: CD - Macromolecular Chemistry

  14. VT Biodiversity Project - Biological Hotspots

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This dataset is the result of an effort to map biological "hotspots" in Vermont based on the "element occurrences" in the Nongame and Natural...

  15. Catalog of research projects at Lawrence Berkeley Laboratory, 1985

    International Nuclear Information System (INIS)

    1985-01-01

    This Catalog has been created to aid in the transfer of technology from the Lawrence Berkeley Laboratory to potential users in industry, government, universities, and the public. The projects are listed for the following LBL groups: Accelerator and Fusion Research Division, Applied Science Division, Biology and Medicine Division, Center for Advanced Materials, Chemical Biodynamics Division, Computing Division, Earth Sciences Division, Engineering and Technical Services Division, Materials and Molecular Research Division, Nuclear Science Division, and Physics Division

  16. Catalog of research projects at Lawrence Berkeley Laboratory, 1985

    Energy Technology Data Exchange (ETDEWEB)

    1985-01-01

    This Catalog has been created to aid in the transfer of technology from the Lawrence Berkeley Laboratory to potential users in industry, government, universities, and the public. The projects are listed for the following LBL groups: Accelerator and Fusion Research Division, Applied Science Division, Biology and Medicine Division, Center for Advanced Materials, Chemical Biodynamics Division, Computing Division, Earth Sciences Division, Engineering and Technical Services Division, Materials and Molecular Research Division, Nuclear Science Division, and Physics Division.

  17. Molecular biology problem solver: a laboratory guide

    National Research Council Canada - National Science Library

    Gerstein, Alan S

    2001-01-01

    ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi Chapter 1. Preparing for Success in the Laboratory Phillip P. Franciskovich . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. Getting What You Need...

  18. Comparison of Learner Involvement in Biology Laboratory ...

    African Journals Online (AJOL)

    Performance in the biology practical paper has been poor at Kenya national examinations in the past five years (1998-2002). This may imply a lack of emphasis on the teaching of biology through the process-based approach. This study investigated the level of learner involvement in biology practical investigations in ...

  19. Collaborative Systems Biology Projects for the Military Medical Community.

    Science.gov (United States)

    Zalatoris, Jeffrey J; Scheerer, Julia B; Lebeda, Frank J

    2017-09-01

    This pilot study was conducted to examine, for the first time, the ongoing systems biology research and development projects within the laboratories and centers of the U.S. Army Medical Research and Materiel Command (USAMRMC). The analysis has provided an understanding of the breadth of systems biology activities, resources, and collaborations across all USAMRMC subordinate laboratories. The Systems Biology Collaboration Center at USAMRMC issued a survey regarding systems biology research projects to the eight U.S.-based USAMRMC laboratories and centers in August 2016. This survey included a data call worksheet to gather self-identified project and programmatic information. The general topics focused on the investigators and their projects, on the project's research areas, on omics and other large data types being collected and stored, on the analytical or computational tools being used, and on identifying intramural (i.e., USAMRMC) and extramural collaborations. Among seven of the eight laboratories, 62 unique systems biology studies were funded and active during the final quarter of fiscal year 2016. Of 29 preselected medical Research Task Areas, 20 were associated with these studies, some of which were applicable to two or more Research Task Areas. Overall, studies were categorized among six general types of objectives: biological mechanisms of disease, risk of/susceptibility to injury or disease, innate mechanisms of healing, diagnostic and prognostic biomarkers, and host/patient responses to vaccines, and therapeutic strategies including host responses to therapies. We identified eight types of omics studies and four types of study subjects. Studies were categorized on a scale of increasing complexity from single study subject/single omics technology studies (23/62) to studies integrating results across two study subject types and two or more omics technologies (13/62). Investigators at seven USAMRMC laboratories had collaborations with systems biology experts

  20. Leaf LIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus.

    Science.gov (United States)

    Craig, Thomas; Holland, Richard; D'Amore, Rosalinda; Johnson, James R; McCue, Hannah V; West, Anthony; Zulkower, Valentin; Tekotte, Hille; Cai, Yizhi; Swan, Daniel; Davey, Robert P; Hertz-Fowler, Christiane; Hall, Anthony; Caddick, Mark

    2017-12-15

    This paper presents Leaf LIMS, a flexible laboratory information management system (LIMS) designed to address the complexity of synthetic biology workflows. At the project's inception there was a lack of a LIMS designed specifically to address synthetic biology processes, with most systems focused on either next generation sequencing or biobanks and clinical sample handling. Leaf LIMS implements integrated project, item, and laboratory stock tracking, offering complete sample and construct genealogy, materials and lot tracking, and modular assay data capture. Hence, it enables highly configurable task-based workflows and supports data capture from project inception to completion. As such, in addition to it supporting synthetic biology it is ideal for many laboratory environments with multiple projects and users. The system is deployed as a web application through Docker and is provided under a permissive MIT license. It is freely available for download at https://leaflims.github.io .

  1. National Storage Laboratory: a collaborative research project

    Science.gov (United States)

    Coyne, Robert A.; Hulen, Harry; Watson, Richard W.

    1993-01-01

    The grand challenges of science and industry that are driving computing and communications have created corresponding challenges in information storage and retrieval. An industry-led collaborative project has been organized to investigate technology for storage systems that will be the future repositories of national information assets. Industry participants are IBM Federal Systems Company, Ampex Recording Systems Corporation, General Atomics DISCOS Division, IBM ADSTAR, Maximum Strategy Corporation, Network Systems Corporation, and Zitel Corporation. Industry members of the collaborative project are funding their own participation. Lawrence Livermore National Laboratory through its National Energy Research Supercomputer Center (NERSC) will participate in the project as the operational site and provider of applications. The expected result is the creation of a National Storage Laboratory to serve as a prototype and demonstration facility. It is expected that this prototype will represent a significant advance in the technology for distributed storage systems capable of handling gigabyte-class files at gigabit-per-second data rates. Specifically, the collaboration expects to make significant advances in hardware, software, and systems technology in four areas of need, (1) network-attached high performance storage; (2) multiple, dynamic, distributed storage hierarchies; (3) layered access to storage system services; and (4) storage system management.

  2. Infusing Bioinformatics and Research-Like Experience into a Molecular Biology Laboratory Course

    Science.gov (United States)

    Nogaj, Luiza A.

    2014-01-01

    A nine-week laboratory project designed for a sophomore level molecular biology course is described. Small groups of students (3-4 per group) choose a tumor suppressor gene (TSG) or an oncogene for this project. Each group researches the role of their TSG/oncogene from primary literature articles and uses bioinformatics engines to find the gene…

  3. Mini-projects in Chemical Engineering Laboratory

    Directory of Open Access Journals (Sweden)

    Angeles Cancela

    2013-03-01

    Full Text Available Chemical engineering laboratory practices based in mini-projects were design and applied the students of forestry engineering in chemical subject. This way of practice reveals a more cooperative learning and a different style of experimentation. The stated goal was to design practices that motivate students and to enable them to develop different skills, including cross teamwork and communication. This paper describes how these practices were developed and the advantages and disadvantages of using this methodology of teaching.

  4. ALPI project at Legnaro National Laboratory

    International Nuclear Information System (INIS)

    Fortuna, G.; Pengo, R.; Bassato, G.; Facco, A.; Favaron, P.; Palmieri, V.; Porcellato, A.M.; Rosa, M.; Tiveron, B.

    1988-01-01

    The conceptual design of a superconducting (linac) booster (named ALPI PROJECT) for the 17 MV XTU-TANDEM of Laboratori Nazionali di Legnaro has been recently accepted by the National Institute of Nuclear Physics as one of the leading projects to be funded in the next five year plan. Money for resonator and cryostat prototypes is already available and the building is going to be funded next January. The project aims at a machine capable of accelerating all the stable isotopes up to Uranium at energies above the Coulomb barrier of very possible ion-ion interaction with beam quality comparable to that of d.c. accelerators. At LNL the advantage of coupling the linac postaccelerator to the 17 MV XTU Tandem is taken which is able to produce even the very heavy beams with reliable intensity and velocities β ≥ 0.04 which can be matched by superconducting resonators feasible with the present available technology. As accelerating structures in the ALPI project straight line quarter wave resonators (QWR) have been chosen on the basis of their intrinsic mechanical stability and broad velocity acceptance (two gap resonator) particularly important for a national facility like ALPI which is expected to produce as many different beams as possible. Lead has been chosen as superconductor on the basis of the following considerations: (i) lead technology being much more applied for QWR resonators than the Nb one can be easier and faster introduced in a Nuclear Physics Laboratory without any experience in the field; (ii) the performances of SUNYLAC have demonstrated that their initial goal of reaching accelerating gradient of 3 MV/m is feasible; (iii) the difficulty in fabricating the OFHC copper base of the resonators (number of EB welds, joints) is relatively modest if compared with the solutions involving Nb as superconductor. 7 references, 3 figures

  5. Biologically inspired technologies in NASA's morphing project

    Science.gov (United States)

    McGowan, Anna-Maria R.; Cox, David E.; Lazos, Barry S.; Waszak, Martin R.; Raney, David L.; Siochi, Emilie J.; Pao, S. Paul

    2003-07-01

    For centuries, biology has provided fertile ground for hypothesis, discovery, and inspiration. Time-tested methods used in nature are being used as a basis for several research studies conducted at the NASA Langley Research Center as a part of Morphing Project, which develops and assesses breakthrough vehicle technologies. These studies range from low drag airfoil design guided by marine and avian morphologies to soaring techniques inspired by birds and the study of small flexible wing vehicles. Biology often suggests unconventional yet effective approaches such as non-planar wings, dynamic soaring, exploiting aeroelastic effects, collaborative control, flapping, and fibrous active materials. These approaches and other novel technologies for future flight vehicles are being studied in NASA's Morphing Project. This paper will discuss recent findings in the aeronautics-based, biologically-inspired research in the project.

  6. Final characterization report for the 108-F Biological Laboratory

    International Nuclear Information System (INIS)

    Harris, R.A.

    1996-09-01

    This report provides a compilation of characterization data for the 108-F Biological Laboratory collected during the period of May 7, 1996 through August 29, 1996. The 108-F Biology Laboratory is located on the Hanford Site in Richland, Washington. The characterization activities were organized and implemented to evaluate the radiological status of the laboratory and to identify hazardous materials. This report reflects the current conditions and status of the laboratory. Information in this report is intended to be utilized to prepare an accurate cost estimate for building demolition, to aid in planning decontamination and demolition activities, and allow proper disposal of demolition debris

  7. Life sciences space biology project planning

    Science.gov (United States)

    Primeaux, G.; Newkirk, K.; Miller, L.; Lewis, G.; Michaud, R.

    1988-01-01

    The Life Sciences Space Biology (LSSB) research will explore the effect of microgravity on humans, including the physiological, clinical, and sociological implications of space flight and the readaptations upon return to earth. Physiological anomalies from past U.S. space flights will be used in planning the LSSB project.The planning effort integrates science and engineering. Other goals of the LSSB project include the provision of macroscopic view of the earth's biosphere, and the development of spinoff technology for application on earth.

  8. Laboratory animal: biological reagent or living being?

    Science.gov (United States)

    Cardoso, C V P; Almeida, A E C C de

    2014-01-01

    The duties of humans toward non-human animals and their rights in society have been debated for a long time. However, a discussion on the terminology used for the identification of laboratory animals is usually not considered, although the employment of inadequate terminology may generate disastrous consequences for the animals before, during, and after the experiment. This study intends to defend the use of appropriate terminology, call attention to an unethical attitude of certain professionals when dealing with experimental animals, and also propose operational mechanisms, which allow for those distortions to be corrected.

  9. FEBEX II Project THG Laboratory Experiments

    International Nuclear Information System (INIS)

    Missana, T.

    2004-01-01

    The main roles of the bentonite in a radioactive waste repository is to act as a geochemical barrier against the radionuclides migration. The effectiveness of this geochemical barrier depends on the surface properties of the solid phases and on the physico-chemical environment generated by the interaction of the solid phases with the groundwater. Within the FEBEX (Full-scale Engineered Barriers Experiment) project, a program of laboratory tests was designed to study and to understand the processes taking place in the clay barrier. Since the first stages of the project, these laboratory tests enabled to isolate different processes, making easier their interpretation, and provided fundamental parameters to be used in the Thermo Hydro Mechanical (THM) and Thermo Hydro Geochemical (THG) models. Additionally, experimental data enabled to check the predictive capability of these models. In the second phase of the project, laboratory tests focused on all those relevant aspects not sufficiently covered during FEBEX I. Particularly, the following main objectives were proposed for the THG investigations during FEBEX II : Attainment of a reliable description of the pore water chemistry at different geochemical conditions. Identification of the different types of water present in the bentonite and to determine the amount of available water for the solute transport.Evaluation of the potential effects of the extraction pressure in the chemical composition of the water obtained by squeezing methods.Study of the effects of the exchange complex in the rheological properties of the clay.Identification and modelling of the surface processes occurring in smectite, determination of the solubility constants of smectite and the formation constants of the surface complexes.Understanding of the mechanisms involved in the sorption of different radionuclides in the bentonite. Investigation of the diffusion mechanisms of conservative neutral and anionic species to have a deeper insight on the

  10. FEBEX II Project THG Laboratory Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Missana, T.

    2004-07-01

    The main roles of the bentonite in a radioactive waste repository is to act as a geochemical barrier against the radionuclides migration. The effectiveness of this geochemical barrier depends on the surface properties of the solid phases and on the physico-chemical environment generated by the interaction of the solid phases with the groundwater. Within the FEBEX (Full-scale Engineered Barriers Experiment) project, a program of laboratory tests was designed to study and to understand the processes taking place in the clay barrier. Since the first stages of the project, these laboratory tests enabled to isolate different processes, making easier their interpretation, and provided fundamental parameters to be used in the Thermo Hydro Mechanical (THM) and Thermo Hydro Geochemical (THG) models. Additionally, experimental data enabled to check the predictive capability of these models. In the second phase of the project, laboratory tests focused on all those relevant aspects not sufficiently covered during FEBEX I. Particularly, the following main objectives were proposed for the THG investigations during FEBEX II : Attainment of a reliable description of the pore water chemistry at different geochemical conditions. Identification of the different types of water present in the bentonite and to determine the amount of available water for the solute transport.Evaluation of the potential effects of the extraction pressure in the chemical composition of the water obtained by squeezing methods.Study of the effects of the exchange complex in the rheological properties of the clay.Identification and modelling of the surface processes occurring in smectite, determination of the solubility constants of smectite and the formation constants of the surface complexes.Understanding of the mechanisms involved in the sorption of different radionuclides in the bentonite. Investigation of the diffusion mechanisms of conservative neutral and anionic species to have a deeper insight on the

  11. From Cookbook to Collaborative: Transforming a University Biology Laboratory Course

    Science.gov (United States)

    Herron, Sherry S.

    2009-01-01

    As described in "How People Learn," "Developing Biological Literacy," and by the Commission on Undergraduate Education in the Biological Sciences during the 1960s and early 1970s, laboratories should promote guided-inquiries or investigations, and not simply consist of cookbook or verification activities. However, the only word that could describe…

  12. Oversight of High-Containment Biological Laboratories: Issues for Congress

    Science.gov (United States)

    2009-05-04

    Laboratories: Issues for Congress Congressional Research Service 14 Industry and Non-Profit Laboratories Private sector companies and non-profit...resources for these endeavors. Whether public or private sector , high-containment laboratories are planned and designed to minimize the possibility of... equine encephalitis, and yellow fever. Some of the pathogens that cause these diseases have been considered as biological weapons.104 Expanding the number

  13. [RESAOLAB: West African network of laboratories to enhance the quality of clinical biology].

    Science.gov (United States)

    Delorme, L; Machuron, J L; Sow, I; Diagne, R; Sakandé, J; Nikiéma, A; Bougoudogo, F; Keita, A; Longuet, C

    2015-02-01

    The Fondation Mérieux, in partnership with the Ministries of Health of Burkina Faso, Mali and Senegal, implemented for four years a project to reinforce the laboratory sector in the three participating countries: the RESAOLAB project (West African Network of Biomedical Analysis Laboratories).The objective of RESAOLAB project, in partnership with the WHO Office for West Africa and the West African Health Organization, was to strengthen the systems of biomedical laboratories to improve diagnostic services, access, monitoring and management of infectious diseases. Following the successful results achieved under the RESAOLAB project and due to the demand of the neighbour countries ministries, the RESAOLAB project is now extended to four other countries of the West African region: Benin, Guinea-Conakry, Niger and Togo. The RESAOLAB project has become the RESAOLAB programme, its purpose is to strengthen the quality of the medical biology services thanks to a regional and transversal approach.

  14. Organization of a radioisotope based molecular biology laboratory

    International Nuclear Information System (INIS)

    2006-12-01

    Polymerase chain reaction (PCR) has revolutionized the application of molecular techniques to medicine. Together with other molecular biology techniques it is being increasingly applied to human health for identifying prognostic markers and drug resistant profiles, developing diagnostic tests and genotyping systems and for treatment follow-up of certain diseases in developed countries. Developing Member States have expressed their need to also benefit from the dissemination of molecular advances. The use of radioisotopes, as a step in the detection process or for increased sensitivity and specificity is well established, making it ideally suitable for technology transfer. Many molecular based projects using isotopes for detecting and studying micro organisms, hereditary and neoplastic diseases are received for approval every year. In keeping with the IAEA's programme, several training activities and seminars have been organized to enhance the capabilities of developing Member States to employ in vitro nuclear medicine technologies for managing their important health problems and for undertaking related basic and clinical research. The background material for this publication was collected at training activities and from feedback received from participants at research and coordination meetings. In addition, a consultants' meeting was held in June 2004 to compile the first draft of this report. Previous IAEA TECDOCS, namely IAEA-TECDOC-748 and IAEA-TECDOC-1001, focused on molecular techniques and their application to medicine while the present publication provides information on organization of the laboratory, quality assurance and radio-safety. The technology has specific requirements of the way the laboratory is organized (e.g. for avoiding contamination and false positives in PCR) and of quality assurance in order to provide accurate information to decision makers. In addition while users of the technology accept the scientific rationale of using radio

  15. Curiosity: the Mars Science Laboratory Project

    Science.gov (United States)

    Cook, Richard A.

    2012-01-01

    The Curiosity rover landed successfully in Gale Crater, Mars on August 5, 2012. This event was a dramatic high point in the decade long effort to design, build, test and fly the most sophisticated scientific vehicle ever sent to Mars. The real achievements of the mission have only just begun, however, as Curiosity is now searching for signs that Mars once possessed habitable environments. The Mars Science Laboratory Project has been one of the most ambitious and challenging planetary projects that NASA has undertaken. It started in the successful aftermath of the 2003 Mars Exploration Rover project and was designed to take significant steps forward in both engineering and scientific capabilities. This included a new landing system capable of emplacing a large mobile vehicle over a wide range of potential landing sites, advanced sample acquisition and handling capabilities that can retrieve samples from both rocks and soil, and a high reliability avionics suite that is designed to permit long duration surface operations. It also includes a set of ten sophisticated scientific instruments that will investigate both the geological context of the landing site plus analyze samples to understand the chemical & organic composition of rocks & soil found there. The Gale Crater site has been specifically selected as a promising location where ancient habitable environments may have existed and for which evidence may be preserved. Curiosity will spend a minimum of one Mars year (about two Earth years) looking for this evidence. This paper will report on the progress of the mission over the first few months of surface operations, plus look retrospectively at lessons learned during both the development and cruise operations phase of the mission..

  16. EDITORIAL: Student undergraduate laboratory and project work

    Science.gov (United States)

    Schumacher, Dieter

    2007-05-01

    that new experiments which illustrate both fundamental physics and modern technology can be realized even with a small budget. Traditional labwork courses often provide a catalogue of well known experiments. The students must first learn the theoretical background. They then assemble the setup from specified equipment, collect the data and perform the default data processing. However, there is no way to learn to swim without water. In order to achieve a constructivist access to learning, 'project labs' are needed. In a project labwork course a small group of students works as a team on a mini research project. The students have to specify the question of research, develop a suitable experimental setup, conduct the experiment and find a suitable way to evaluate the data. Finally they must present their results e.g. in the framework of a public poster session. Three contributions refer to this approach, however they focus on different aspects: 'Project laboratory for first-year students' by Gorazd Planinšič, 'RealTime Physics: active learning laboratories' by David Sokoloff et al and 'Labs outside labs: miniprojects at a spring camp for future physics teachers' by Leos Dvorák. Is it possible to prepare the students specifically for project labwork? This question is answered by the contribution 'A new labwork course for physics students: devices, methods and research projects' by Knut Neumann and Manuela Welzel. The two main parts of the labwork course cover first experimental devices (e.g. multimeters, oscilloscopes, different sensors, operational amplifiers, step motors, AD/DA-converters). Then subjects such as data processing, consideration of measurement uncertainties, keeping records or using tools like LABVIEW etc are focused on. Another concrete proposal for a new curriculum is provided by James Sharp et al, in 'Computer based learning in an undergraduate physics laboratory: interfacing and instrument control using MATLAB'. One can well imagine that project labs

  17. Establishing a national biological laboratory safety and security monitoring program.

    Science.gov (United States)

    Blaine, James W

    2012-12-01

    The growing concern over the potential use of biological agents as weapons and the continuing work of the Biological Weapons Convention has promoted an interest in establishing national biological laboratory biosafety and biosecurity monitoring programs. The challenges and issues that should be considered by governments, or organizations, embarking on the creation of a biological laboratory biosafety and biosecurity monitoring program are discussed in this article. The discussion focuses on the following questions: Is there critical infrastructure support available? What should be the program focus? Who should be monitored? Who should do the monitoring? How extensive should the monitoring be? What standards and requirements should be used? What are the consequences if a laboratory does not meet the requirements or is not willing to comply? Would the program achieve the results intended? What are the program costs? The success of a monitoring program can depend on how the government, or organization, responds to these questions.

  18. The ATLAS project: The effects of a constructionist digital laboratory project on undergraduate laboratory performance.

    Science.gov (United States)

    Shoepe, Todd C; Cavedon, Dana K; Derian, Joseph M; Levy, Celine S; Morales, Amy

    2015-01-01

    Anatomical education is a dynamic field where developments in the implementation of constructive, situated-learning show promise in improving student achievement. The purpose of this study was to examine the effectiveness of an individualized, technology heavy project in promoting student performance in a combined anatomy and physiology laboratory course. Mixed-methods research was used to compare two cohorts of anatomy laboratories separated by the adoption of a new laboratory atlas project, which were defined as preceding (PRE) and following the adoption of the Anatomical Teaching and Learning Assessment Study (ATLAS; POST). The ATLAS project required the creation of a student-generated, photographic atlas via acquisition of specimen images taken with tablet technology and digital microscope cameras throughout the semester. Images were transferred to laptops, digitally labeled and photo edited weekly, and compiled into a digital book using Internet publishing freeware for final project submission. An analysis of covariance confirmed that student final examination scores were improved (P project (PRE, n = 75; POST, n = 90; means ± SE; 74.9 ± 0.9 versus 78.1 ± 0.8, respectively) after controlling for cumulative student grade point average. Analysis of questionnaires collected (n = 68) from the post group suggested students identified with atlas objectives, appreciated the comprehensive value in final examination preparation, and the constructionism involved, but recommended alterations in assignment logistics and the format of the final version. Constructionist, comprehensive term-projects utilizing student-preferred technologies could be used to improve performance toward student learning outcomes. © 2014 American Association of Anatomists.

  19. Semester-Long Inquiry-Based Molecular Biology Laboratory: Transcriptional Regulation in Yeast

    Science.gov (United States)

    Oelkers, Peter M.

    2017-01-01

    A single semester molecular biology laboratory has been developed in which students design and execute a project examining transcriptional regulation in "Saccharomyces cerevisiae." Three weeks of planning are allocated to developing a hypothesis through literature searches and use of bioinformatics. Common experimental plans address a…

  20. A qualitative characterization of an introductory college nonmajors biology laboratory

    Science.gov (United States)

    Lee, Cherin Ann

    The nature of an undergraduate, nonmajors biology laboratory was investigated in this study. Student participants were enrolled in a general education biology laboratory course at the University of Northern Iowa. The researcher's purpose was to gain a characterization of the instructional format and laboratory activities experienced by students. Interpretation of student and instructor responses enabled an insider's view of the biology laboratory. The laboratory period was consistently described by both students and instructors as having three parts, Beginning, Middle, and End, with the End being of special importance for conceptual development. The instructional format of the three instructors differed within the three portions of the laboratory period, ranging from an inquiry-oriented, partial learning cycle to a fairly expository model labeled inform/verify/practice. There was striking similarity in intrasectional student and teacher descriptions of instructional format. Additionally, students experiencing the alternate instructor provided the same characterizations of instructional format as those provided by the instructor's usual students. There were no discernible patterns of instructional format based on sex or reasoning level. In addition to the central role of instructional format, three areas of importance emerged: the social aspects of learning, the collaborative and cooperative nature of laboratory work and learning, and the role of self-efficacy. Theory developed from and grounded in the data showed six factors important in the introductory college biology laboratory: collaborative and cooperative learning, student-student and teacher-student interactions, attitude and self-efficacy, learning process and learning style, effective instructional format, and science content. These factors were found to be similar to factors identified in the literature as important in K-12 science education. These factors were set in the context of schooling and learning

  1. Sandia, California Tritium Research Laboratory transition and reutilization project

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, T.B. [Sandia National Lab., Albuquerque, NM (United States)

    1997-02-01

    This paper describes a project within Sandia National Laboratory to convert the shut down Tritium Research Laboratory into a facility which could be reused within the laboratory complex. In the process of decommissioning and decontaminating the facility, the laboratory was able to save substantial financial resources by transferring much existing equipment to other DOE facilities, and then expeditiously implementing a decontamination program which has resulted in the building being converted into laboratory space for new lab programs. This project of facility reuse has been a significant financial benefit to the laboratory.

  2. Structural biology facilities at Brookhaven National Laboratory`s high flux beam reactor

    Energy Technology Data Exchange (ETDEWEB)

    Korszun, Z.R.; Saxena, A.M.; Schneider, D.K. [Brookhaven National Laboratory, Upton, NY (United States)

    1994-12-31

    The techniques for determining the structure of biological molecules and larger biological assemblies depend on the extent of order in the particular system. At the High Flux Beam Reactor at the Brookhaven National Laboratory, the Biology Department operates three beam lines dedicated to biological structure studies. These beam lines span the resolution range from approximately 700{Angstrom} to approximately 1.5{Angstrom} and are designed to perform structural studies on a wide range of biological systems. Beam line H3A is dedicated to single crystal diffraction studies of macromolecules, while beam line H3B is designed to study diffraction from partially ordered systems such as biological membranes. Beam line H9B is located on the cold source and is designed for small angle scattering experiments on oligomeric biological systems.

  3. Biological and Physical Space Research Laboratory 2002 Science Review

    Science.gov (United States)

    Curreri, P. A. (Editor); Robinson, M. B. (Editor); Murphy, K. L. (Editor)

    2003-01-01

    With the International Space Station Program approaching core complete, our NASA Headquarters sponsor, the new Code U Enterprise, Biological and Physical Research, is shifting its research emphasis from purely fundamental microgravity and biological sciences to strategic research aimed at enabling human missions beyond Earth orbit. Although we anticipate supporting microgravity research on the ISS for some time to come, our laboratory has been vigorously engaged in developing these new strategic research areas.This Technical Memorandum documents the internal science research at our laboratory as presented in a review to Dr. Ann Whitaker, MSFC Science Director, in July 2002. These presentations have been revised and updated as appropriate for this report. It provides a snapshot of the internal science capability of our laboratory as an aid to other NASA organizations and the external scientific community.

  4. Myoglobin Structure and Function: A Multiweek Biochemistry Laboratory Project

    Science.gov (United States)

    Silverstein, Todd P.; Kirk, Sarah R.; Meyer, Scott C.; Holman, Karen L. McFarlane

    2015-01-01

    We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure,…

  5. Professor Created On-line Biology Laboratory Course

    Science.gov (United States)

    Bowman, Arthur W.

    2010-01-01

    This paper will share the creation, implementation, and modification of an online college level general biology laboratory course offered for non-science majors as a part of a General Education Curriculum. The ability of professors to develop quality online laboratories will address a growing need in Higher Education as more institutions combine course sections and look for suitable alternative course delivery formats due to declining departmental budgets requiring reductions in staffing, equipment, and supplies. Also, there is an equal or greater need for more professors to develop the ability to create online laboratory experiences because many of the currently available online laboratory course packages from publishers do not always adequately parallel on-campus laboratory courses, or are not as aligned with the companion lecture sections. From a variety of scientific simulation and animation web sites, professors can easily identify material that closely fit the specific needs of their courses, instructional environment, and students that they serve. All too often, on-campus laboratory courses in the sciences provide what are termed confirmation experiences that do NOT allow students to experience science as would be carried out by scientists. Creatively developed online laboratory experiences can often provide the type of authentic investigative experiences that are not possible on-campus due to the time constraints of a typical two-hour, once-per-week-meeting laboratory course. In addition, online laboratory courses can address issues related to the need for students to more easily complete missing laboratory assignments, and to have opportunities to extend introductory exercises into more advanced undertakings where a greater sense of scientific discovery can be experienced. Professors are strongly encourages to begin creating online laboratory exercises for their courses, and to consider issues regarding assessment, copyrights, and Intellectual Property

  6. Removal design report for the 108-F Biological Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-09-01

    Most of the 100-F facilities were deactivated with the reactor and have since been demolished. Of the dozen or so reactor-related structures, only the 105-F Reactor Building and the 108-F Biology Laboratory remain standing today. The 108-F Biology Laboratory was intended to be used as a facility for the mixing and addition of chemicals used in the treatment of the reactor cooling water. Shortly after F Reactor began operation, it was determined that the facility was not needed for this purpose. In 1949, the building was converted for use as a biological laboratory. In 1962, the lab was expanded by adding a three-story annex to the original four-story structure. The resulting lab had a floor area of approximately 2,883 m{sup 2} (main building and annex) that operated until 1973. The building contained 47 laboratories, a number of small offices, a conference room, administrative section, lunch and locker rooms, and a heavily shielded, high-energy exposure cell. The purpose of this removal design report is to establish the methods of decontamination and decommissioning and the supporting functions associated with facility removal and disposal.

  7. Removal design report for the 108-F Biological Laboratory

    International Nuclear Information System (INIS)

    1997-09-01

    Most of the 100-F facilities were deactivated with the reactor and have since been demolished. Of the dozen or so reactor-related structures, only the 105-F Reactor Building and the 108-F Biology Laboratory remain standing today. The 108-F Biology Laboratory was intended to be used as a facility for the mixing and addition of chemicals used in the treatment of the reactor cooling water. Shortly after F Reactor began operation, it was determined that the facility was not needed for this purpose. In 1949, the building was converted for use as a biological laboratory. In 1962, the lab was expanded by adding a three-story annex to the original four-story structure. The resulting lab had a floor area of approximately 2,883 m 2 (main building and annex) that operated until 1973. The building contained 47 laboratories, a number of small offices, a conference room, administrative section, lunch and locker rooms, and a heavily shielded, high-energy exposure cell. The purpose of this removal design report is to establish the methods of decontamination and decommissioning and the supporting functions associated with facility removal and disposal

  8. U1/U2 crib groundwater biological treatment demonstration project

    International Nuclear Information System (INIS)

    Koegler, S.S.; Brouns, T.M.; Heath, W.O.

    1989-11-01

    The primary objective of the biological treatment project is to develop and demonstrate a process for Hanford groundwater remediation. Biodenitrification using facultative anaerobic microorganisms is a promising technology for the simultaneous removal of nitrates and organics from contaminated aqueous streams. During FY 1988, a consortium of Hanford groundwater microorganisms was shown to degrade both nitrates and carbon tetrachloride (CC1 4 ). A pilot-scale treatment system was designed and constructed based on the results of laboratory-and-bench-scale testing. This report summarizes the results of biological groundwater treatment studies performed during FY 1989 at the pilot-scale. These tests were conducted using a simulated Hanford groundwater with a continuous stirred-tank bioreactor, and a fluidized-bed bioreactor that was added to the pilot-scale treatment system in FY 1989. The pilot-scale system demonstrated continuous degradation of nitrates and CC1 4 in a simulated groundwater. 4 refs., 7 figs., 1 tab

  9. A Guided-Inquiry pH Laboratory Exercise for Introductory Biological Science Laboratories

    Science.gov (United States)

    Snodgrass, Meagan A.; Lux, Nicholas; Metz, Anneke M.

    2011-01-01

    There is a continuing need for engaging inquiry-based laboratory experiences for advanced high school and undergraduate biology courses. The authors describe a guided-inquiry exercise investigating the pH-dependence of lactase enzyme that uses an inexpensive, wide-range buffering system, lactase dietary supplement, over-the-counter glucose test…

  10. Moonshot Laboratories' Lava Relief Google Mapping Project

    Science.gov (United States)

    Brennan, B.; Tomita, M.

    2016-12-01

    The Moonshot Laboratories were conceived at the University Laboratory School (ULS) on Oahu, Hawaii as way to develop creative problem solvers able to resourcefully apply 21st century technologies to respond to the problems and needs of their communities. One example of this was involved students from ULS using modern mapping and imaging technologies to assist peers who had been displaced from their own school in Pahoe on the Big Island of Hawaii. During 2015, lava flows from the eruption of Kilauea Volcano were slowly encroaching into the district of Puna in 2015. The lava flow was cutting the main town of Pahoa in half, leaving no safe routes of passage into or out of the town. One elementary school in the path of the flow was closed entirely and a new one was erected north of the flow for students living on that side. Pahoa High School students and teachers living to the north were been forced to leave their school and transfer to Kea'au High School. These students were separated from friends, family and the community they grew up in and were being thrust into a foreign environment that until then had been their local rival. Using Google Mapping technologies, Moonshot Laboratories students created a dynamic map to introduce the incoming Pahoa students to their new school in Kea'au. Elements included a stylized My Maps basemap, YouTube video descriptions of the building, videos recorded by Google Glass showing first person experiences, and immersive images of classrooms were created using 360 cameras. During the first day of orientation at Kea'au for the 200 Pahoa students, each of them were given a tablet to view the map as they toured and got to know their new campus. The methods and technologies, and more importantly innovative thinking, used to create this map have enormous potential for how to educate all students about the world around us, and the issues facing it. http://www.moonshotincubator.com/

  11. Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course

    Science.gov (United States)

    MacDonald, Gina

    2008-01-01

    This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques…

  12. The science of laboratory and project management in regulated bioanalysis.

    Science.gov (United States)

    Unger, Steve; Lloyd, Thomas; Tan, Melvin; Hou, Jingguo; Wells, Edward

    2014-05-01

    Pharmaceutical drug development is a complex and lengthy process, requiring excellent project and laboratory management skills. Bioanalysis anchors drug safety and efficacy with systemic and site of action exposures. Development of scientific talent and a willingness to innovate or adopt new technology is essential. Taking unnecessary risks, however, should be avoided. Scientists must strategically assess all risks and find means to minimize or negate them. Laboratory Managers must keep abreast of ever-changing technology. Investments in instrumentation and laboratory design are critical catalysts to efficiency and safety. Matrix management requires regular communication between Project Managers and Laboratory Managers. When properly executed, it aligns the best resources at the right times for a successful outcome. Attention to detail is a critical aspect that separates excellent laboratories. Each assay is unique and requires attention in its development, validation and execution. Methods, training and facilities are the foundation of a bioanalytical laboratory.

  13. Project-Based Laboratory Experiences in Mechanical Engineering

    Directory of Open Access Journals (Sweden)

    Narendra Sharma

    2011-12-01

    Full Text Available In this paper we describe project-based laboratories in Mechanical Engineering designed to provide semester-long team experiences which mimic the real life industrial processes of design, development, testing and optimization. The labs are focused on courses at the sophomore level and thus require special attention to constraints of student backgrounds and experience. This paper describes laboratory projects in Dynamics and Fluid Mechanics.

  14. Atmospheric cloud physics laboratory project study

    Science.gov (United States)

    Schultz, W. E.; Stephen, L. A.; Usher, L. H.

    1976-01-01

    Engineering studies were performed for the Zero-G Cloud Physics Experiment liquid cooling and air pressure control systems. A total of four concepts for the liquid cooling system was evaluated, two of which were found to closely approach the systems requirements. Thermal insulation requirements, system hardware, and control sensor locations were established. The reservoir sizes and initial temperatures were defined as well as system power requirements. In the study of the pressure control system, fluid analyses by the Atmospheric Cloud Physics Laboratory were performed to determine flow characteristics of various orifice sizes, vacuum pump adequacy, and control systems performance. System parameters predicted in these analyses as a function of time include the following for various orifice sizes: (1) chamber and vacuum pump mass flow rates, (2) the number of valve openings or closures, (3) the maximum cloud chamber pressure deviation from the allowable, and (4) cloud chamber and accumulator pressure.

  15. Biology of Dermacentor marginatus (Acari: Ixodidae under laboratory conditions

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Darvishi

    2014-02-01

    Full Text Available Objective: To investigate and survey the biology of Dermacentor marginatus (D. marginatus under laboratory conditions. Methods: In this investigation, D. marginatus adult ticks were collected from sheep in Semnan province. Then various developmental stages of D. marginatus including larvae, nymphs and adult males and females under laboratory condition were bred and their biology was scrutinized. Results: The requisite time to complete the life cycle of D. marginatus under controlled laboratory conditions for larvae (26 °C, 75% relative humidity and nymph (26 °C, 95% relative humidity moulting, was on average 92 d (range 75-104 d, including preoviposition and egg incubation (22.5 d, larvae incubation (20.5 d, nymphal stage (28 d along with male maturation (21 d. The index of conversion efficiency and the index of reproduction efficiency in females were 0.397 and 8.300, respectively. Conclusions: Although in this investigation, there was no meaningful correlation between preoviposition period and the weight of female ticks which were laid successfully. The significant linear relationship was fully observed between the weight of engorged female of D. marginatus and the number of eggs laid. The mean of preoviposition period from 5.4 d in autumn to 34.2 d in spring increased. The minimum weight of ticks with laying capacity was 69 mg and lighter ticks (21 mg either did not lay or if they did their eggs did not hatch.

  16. Successful neural network projects at the Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    Cordes, G.A.

    1991-01-01

    This paper presents recent and current projects at the Idaho National Engineering Laboratory (INEL) that research and apply neural network technology. The projects are summarized in the paper and their direct application to space reactor power and propulsion systems activities is discussed. 9 refs., 10 figs., 3 tabs

  17. [Hygiene and security management in medical biology laboratory].

    Science.gov (United States)

    Vinner, E; Odou, M F; Fovet, B; Ghnassia, J C

    2013-06-01

    Risk management in Medical Biology Laboratory (MBL) which includes hygiene and waste management, is an integrated process to the whole MBL organisation. It is composed of three stages: risks factors identification, grading and prioritization, and their evaluation in the system. From the legislation and NF EN ISO 15189 standard's requirements viewpoint, prevention and protection actions to implement are described, at premises level, but also at work station environment's one (human resources and equipments) towards biological, chemical, linked to gas, to ionizing or non ionizing radiations and fire riks, in order not to compromise patients safety, employees safety, and quality results. Then, although NF EN 15189 standard only enacts requirements in terms of prevention, curative actions after established blood or chemical exposure accident are defined.

  18. Project of an integrated calibration laboratory of instruments at IPEN

    International Nuclear Information System (INIS)

    Barros, Gustavo Adolfo San Jose

    2009-01-01

    The Calibration Laboratory of Instruments of Instituto de Pesquisas Energeticas e Nucleares offers calibration services of radiation detectors used in radioprotection, diagnostic radiology and radiotherapy, for IPEN and for external facilities (public and private). One part of its facilities is located in the main building, along with other laboratories and study rooms, and another part in an isolated building called Bunker. For the optimization, modernization and specially the safety, the laboratories in the main building shall be transferred to an isolated place. In this work, a project of an integrated laboratory for calibration of instruments was developed, and it will be an expansion of the current Calibration Laboratory of Instruments of IPEN. Therefore, a series of radiometric monitoring of the chosen localization of the future laboratory was realized, and all staff needs (dimensions and disposition of the study rooms and laboratories) were defined. In this project, the laboratories with X ray equipment, alpha and beta radiation sources were located at an isolated part of the building, and the wall shielding was determined, depending on the use of each laboratory. (author)

  19. [Japanese sentinel project and contribution of laboratory medicine].

    Science.gov (United States)

    Nakashima, Naoki

    2013-06-01

    Ordinary passive surveillance (generally spontaneous reporting) of adverse effects of medical products is not enough to manage medical risks/safety. The Japanese government, following the "sentinel initiative" by the US government, started the "Japanese sentinel project" as an active surveillance of medical products with the Pharmaceuticals and Medical Devices Agency(PMDA). Using entire prescription data in the hospital information system, data on laboratory examinations, diagnosis, and therapy specific to an adverse effect are checked by detecting differences between before and after prescription of a specific medical product to extract adverse effects and calculate their correct frequency in the surveillance. The project uses standard masters and protocols to analyze data among medial institutes by installation of the SS-MIX standard storage system. Ten hospitals were selected in 2011 to join the project, and the entire system and network will be implemented in those hospitals and PMDA until 2013 fiscal year. JLAC10 is used in the project as the laboratory examination code of the Japanese Society of Laboratory Medicine. Many incorrect coding and ambiguous coding rules were found by voluntary surveillance of 6 of the 10 hospitals in the project. Thus, the Council on Standardized Master Operation of Clinical Laboratory Examinations will launch in 2013 to improve and maintain JLAC10, which is essential not only for the Japanese sentinel project but also for data utilization by many other projects.

  20. Biological aspects of Hylesia metapyrrha (Lepidoptera; Saturniidae; Hemileucinae, in laboratory

    Directory of Open Access Journals (Sweden)

    A. Specht

    Full Text Available The aim of this work was to study biological aspects and the life cycle of Hylesia Metapyrrha in a laboratory. Laboratorial breeding was made at 25 ± 1 °C, 70 ± 10% UR and 14 hours of photophase, feeding the larvae with guava leaves (Psidium guayava L. - Myrtaceae. Time was evaluated on the days of all the development stages; morphometry was evaluated in millimeters and the pupa’s mass in grams. The eggs were disposed in groups and covered by urticating abdominal hair. The incubation period lasted 52 days. The larvae, with gregarious habits, presented background black coloration, yellowish scoli and two orange longitudinal lines above and below the spiracles, during the development which lasted an average period of 74.59 days and went through seven instars. The pre-pupa and the pupa stages lasted on average 8.82 and 50.56 days, respectively; the female pupae presented a duration, weight and size which was significantly bigger. The adult stage lasted on average 5.50 days with periods of pre, post and oviposition of 2.30, 1.90 and 1.00 days, respectively. This study broadens the knowledge of the immature stages, biological, morphological and behavioral aspects, until then restricted to the morphology and to registers of the occurrence of the adult forms.

  1. Laboratory Technology Research: Abstracts of FY 1996 projects

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-31

    The Laboratory Technology Research (LTR) program supports high-risk, multidisciplinary research partnerships to investigate challenging scientific problems whose solutions have promising commercial potential. These partnerships capitalize on two great strengths of this country: the world-class basic research capability of the DOE Energy Research (ER) multi-program national laboratories and the unparalleled entrepreneurial spirit of American industry. Projects supported by the LTR program are conducted by the five ER multi-program laboratories: Argonne, Brookhaven, Lawrence Berkeley, Oak Ridge, and Pacific Northwest National Laboratories. These projects explore the applications of basic research advances relevant to Department of Energy`s (DOE) mission over a full range of scientific disciplines. The program presently emphasizes three critical areas of mission-related research: advanced materials, intelligent processing/manufacturing research, and sustainable environments.

  2. State of laboratory manual instruction in California community college introductory (non-majors) biology laboratory instruction

    Science.gov (United States)

    Priest, Michelle

    College students must complete a life science course prior to graduation for a bachelor's degree. Generally, the course has lecture and laboratory components. It is in the laboratory where there are exceptional opportunities for exploration, challenge and application of the material learned. Optimally, this would utilize the best of inquiry based approaches. Most community colleges are using a home-grown or self written laboratory manual for the direction of work in the laboratory period. Little was known about the motivation, development and adaptation of use. It was also not known about the future of the laboratory manuals in light of the recent learning reform in California Community Colleges, Student Learning Outcomes. Extensive interviews were conducted with laboratory manual authors to determine the motivation, process of development, who was involved and learning framework used in the creation of the manuals. It was further asked of manual authors their ideas about the future of the manual, the development of staff and faculty and finally, the role Student Learning Outcomes would play in the manual. Science faculty currently teaching the non-majors biology laboratories for at least two semesters were surveyed on-line about actual practice of the manual, assessment, manual flexibility, faculty training and incorporation of Student Learning Outcomes. Finally, an evaluation of the laboratory manual was done using an established Laboratory Task Analysis Instrument. Laboratory manuals were evaluated on a variety of categories to determine the level of inquiry instruction done by students in the laboratory section. The results were that the development of homegrown laboratory manuals was done by community colleges in the Los Angeles and Orange Counties in an effort to minimize the cost of the manual to the students, to utilize all the exercises in a particular lab and to effectively utilize the materials already owned by the department. Further, schools wanted to

  3. Experiential learning in control systems laboratories and engineering project management

    Science.gov (United States)

    Reck, Rebecca Marie

    2015, a panel of 40 control systems faculty members, from a variety of institutions, completed a multi-round Delphi survey in order to bring them toward consensus on the common aspects of their laboratories. The following winter, 45 additional faculty members and practitioners from the control systems community completed a follow-up survey to gather feedback on the results of the Delphi survey. During the Delphi study, the panelists identified 15 laboratory objectives, 26 concepts, and 15 components that were common in their laboratories. Then in both the Delphi survey and follow-up survey each participant rated the importance of each of these items. While the average ratings differed slightly between the two groups, the order of each set of items was compared with two different tests and the order was found to be similar. Some of the common and important learning objectives include connecting theory to what is implemented and observed in the laboratory, designing controllers, and modeling and simulating systems. The most common component in both groups was Math-Works software. Some of the common concepts include block diagrams, stability, and PID control. Defining common aspects of undergraduate control systems laboratories enables common development, detailed comparisons, and simplified adaptation of equipment and experiments between campuses and programs. Throughout an undergraduate program in engineering, there are multiple opportunities for hands-on laboratory experiences that are related to course content. However, a similarly immersive experience for project management graduate students is harder to incorporate for all students in a course at once. This study explores an experiential learning opportunity for graduate students in engineering management or project management programs. The project management students enroll in a project management course. Undergraduate students interested in working on a project with a real customer enroll in a different projects

  4. The Environmental and Molecular Sciences Laboratory project -- Continuous evolution in leadership

    International Nuclear Information System (INIS)

    Knutson, D.E.; McClusky, J.K.

    1994-10-01

    The Environmental and Molecular Sciences Laboratory (EMSL) construction project at Pacific Northwest Laboratory (PNL) in Richland, Washington, is a $230M Major Systems Acquisition for the US Department of Energy (DOE). The completed laboratory will be a national user facility that provides unparalleled capabilities for scientists involved in environmental molecular science research. This project, approved for construction by the Secretary of Energy in October 1993, is underway. The United States is embarking on an environmental cleanup effort that dwarfs previous scientific enterprise. Using current best available technology, the projected costs of cleaning up the tens of thousands of toxic waste sites, including DOE sites, is estimated to exceed one trillion dollars. The present state of scientific knowledge regarding the effects of exogenous chemicals on human biology is very limited. Long term environmental research at the molecular level is needed to resolve the concerns, and form the building blocks for a structure of cost effective process improvement and regulatory reform

  5. The Environmental and Molecular Sciences Laboratory project -- Continuous evolution in leadership

    Energy Technology Data Exchange (ETDEWEB)

    Knutson, D.E.; McClusky, J.K.

    1994-10-01

    The Environmental and Molecular Sciences Laboratory (EMSL) construction project at Pacific Northwest Laboratory (PNL) in Richland, Washington, is a $230M Major Systems Acquisition for the US Department of Energy (DOE). The completed laboratory will be a national user facility that provides unparalleled capabilities for scientists involved in environmental molecular science research. This project, approved for construction by the Secretary of Energy in October 1993, is underway. The United States is embarking on an environmental cleanup effort that dwarfs previous scientific enterprise. Using current best available technology, the projected costs of cleaning up the tens of thousands of toxic waste sites, including DOE sites, is estimated to exceed one trillion dollars. The present state of scientific knowledge regarding the effects of exogenous chemicals on human biology is very limited. Long term environmental research at the molecular level is needed to resolve the concerns, and form the building blocks for a structure of cost effective process improvement and regulatory reform.

  6. The Dynamics of Project-Based Learning Extension Courses: The "Laboratory of Social Projects" Case Study

    Science.gov (United States)

    Arantes do Amaral, Joao Alberto

    2017-01-01

    In this case study we discuss the dynamics that drive a free-of-charge project-based learning extension course. We discuss the lessons learned in the course, "Laboratory of Social Projects." The course aimed to teach project management skills to the participants. It was conducted from August to November of 2015, at Federal University of…

  7. Team Research at the Biology-Mathematics Interface: Project Management Perspectives

    Science.gov (United States)

    Milton, John G.; Radunskaya, Ami E.; Lee, Arthur H.; de Pillis, Lisette G.; Bartlett, Diana F.

    2010-01-01

    The success of interdisciplinary research teams depends largely upon skills related to team performance. We evaluated student and team performance for undergraduate biology and mathematics students who participated in summer research projects conducted in off-campus laboratories. The student teams were composed of a student with a mathematics…

  8. Siberian Chemical Combine laboratory project work plan, fiscal year 1999

    International Nuclear Information System (INIS)

    Morgado, R.E.; Acobyan, R.; Shropsire, R.

    1998-01-01

    The Siberian Chemical Combine (SKhK), Laboratory Project Work Plan (Plan) is intended to assist the US Laboratory Project Team, and Department of Energy (DOE) staff with the management of the FY99 joint material protection control and accounting program (MPC and A) for enhancing nuclear material safeguards within the Siberian Chemical Combine. The DOE/Russian/Newly Independent States, Nuclear Material Task Force, uses a project work plan document for higher-level program management. The SKhK Plan is a component of the Russian Defense related Sites' input to that document. In addition, it contains task descriptions and a Gantt Chart covering the FY99 time-period. This FY99 window is part of a comprehensive, Project Status Gantt Chart for tasking and goal setting that extends to the year 2003. Secondary and tertiary levels of detail are incorporated therein and are for the use of laboratory project management. The SKhK Plan is a working document, and additions and modifications will be incorporated as the MPC and A project for SKhK evolves

  9. Renewable generation and storage project industry and laboratory recommendations

    Energy Technology Data Exchange (ETDEWEB)

    Clark, N.H.; Butler, P.C.; Cameron, C.P.

    1998-03-01

    The US Department of Energy Office of Utility Technologies is planning a series of related projects that will seek to improve the integration of renewable energy generation with energy storage in modular systems. The Energy Storage Systems Program and the Photovoltaics Program at Sandia National Laboratories conducted meetings to solicit industry guidance and to create a set of recommendations for the proposed projects. Five possible projects were identified and a three pronged approach was recommended. The recommended approach includes preparing a storage technology handbook, analyzing data from currently fielded systems, and defining future user needs and application requirements.

  10. The Los Alamos National Laboratory Nuclear Vision Project

    International Nuclear Information System (INIS)

    Arthur, E.D.; Wagner, R.L. Jr.

    1996-01-01

    Los Alamos National Laboratory has initiated a project to examine possible futures associated with the global nuclear enterprise over the course of the next 50 years. All major components are included in this study--weapons, nonproliferation, nuclear power, nuclear materials, and institutional and public factors. To examine key issues, the project has been organized around three main activity areas--workshops, research and analyses, and development of linkages with other synergistic world efforts. This paper describes the effort--its current and planned activities--as well as provides discussion of project perspectives on nuclear weapons, nonproliferation, nuclear energy, and nuclear materials focus areas

  11. Tritium research laboratory cleanup and transition project final report

    International Nuclear Information System (INIS)

    Johnson, A.J.

    1997-02-01

    This Tritium Research Laboratory Cleanup and Transition Project Final Report provides a high-level summary of this project's multidimensional accomplishments. Throughout this report references are provided for in-depth information concerning the various topical areas. Project related records also offer solutions to many of the technical and or administrative challenges that such a cleanup effort requires. These documents and the experience obtained during this effort are valuable resources to the DOE, which has more than 1200 other process contaminated facilities awaiting cleanup and reapplication or demolition

  12. Biological effects of high-strength electric fields on small laboratory animals. Interim report, March 1, 1978-September 30, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Phillips, R.D.; Anderson, L.E.; Kaune, W.T.

    1979-12-01

    Progress is described on a project assessing the biological effects of 60-Hz electric fields on small laboratory animals (rats and mice). The report includes sections on hematology and seram chemistry, immunology, pathology, metabolism, bone growth, endocrinology, cardiovascular function, neurophysiology, growth and development, and animal behavior. (ACR)

  13. Characterization of Pathogenic Human MSH2 Missense Mutations Using Yeast as a Model System: A Laboratory Course in Molecular Biology

    Science.gov (United States)

    Gammie, Alison E.; Erdeniz, Naz

    2004-01-01

    This work describes the project for an advanced undergraduate laboratory course in cell and molecular biology. One objective of the course is to teach students a variety of cellular and molecular techniques while conducting original research. A second objective is to provide instruction in science writing and data presentation by requiring…

  14. Remote Laboratory Java Server Based on JACOB Project

    Directory of Open Access Journals (Sweden)

    Pavol Bisták

    2011-02-01

    Full Text Available Remote laboratories play an important role in the educational process of engineers. This paper deals with the structure of remote laboratories. The principle of the proposed remote laboratory structure is based on the Java server application that communicates with Matlab through the COM technology for the data exchange under the Windows operating system. Java does not support COM directly so the results of the JACOB project are used and modified to cope with this problem. In laboratories for control engineering education a control algorithm usually runs on a PC with Matlab that really controls the real plant. This is the server side described in the paper in details. To demonstrate the possibilities of a remote control a Java client server application is also introduced. It covers communication and offers a user friendly interface for the control of a remote plant and visualization of measured data.

  15. A semester-long project-oriented biochemistry laboratory based on Helicobacter pylori urease.

    Science.gov (United States)

    Farnham, Kate R; Dube, Danielle H

    2015-01-01

    Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically relevant enzyme--Helicobacter pylori (Hp) urease--the activity of which is absolutely required for the gastric pathogen Hp to colonize the human stomach. Over the course of the semester, students undertake a biochemical purification of Hp urease, assess the success of their purification, and investigate the activity of their purified enzyme. In the final weeks of the semester, students design and implement their own experiments to study Hp urease. This laboratory provides students with an understanding of the importance of biochemistry in human health while empowering them to engage in an active area of research. © 2015 The International Union of Biochemistry and Molecular Biology.

  16. System biology and the project Encode

    Directory of Open Access Journals (Sweden)

    M. Yu. Obolenskaya

    2014-08-01

    Full Text Available The goal of this review is to give an incipient knowledge on the background of system biology, the premises to its assignment as a new branch of biology, its principles, methodology and its great achievements in identification of functional elements of human genome and regulation of their concordant­ and differential activity. The short characteristics of functional elements including the protein-coding sequences and those coding noncoding RNAs, the DNAse 1 hypersensitivity sites and methylated CpG islets, modified histones and specific 3D structure of chromatin, are represented. The topology of transcription factors network with its main motifs, hierar­chy, combination and association of transcription factors and their allelic specificity are highlighted­.

  17. The Human Genome Project and Biology Education.

    Science.gov (United States)

    McInerney, Joseph D.

    1996-01-01

    Highlights the importance of the Human Genome Project in educating the public about genetics. Discusses four challenges that science educators must address: teaching for conceptual understanding, the nature of science, the personal and social impact of science and technology, and the principles of technology. Contains 45 references. (JRH)

  18. [Psychoactive substances in biological samples--toxicological laboratory data].

    Science.gov (United States)

    Gomółka, Ewa; Wilimowska, Jolanta; Piekoszewski, Wojciech; Groszek, Barbara

    2004-01-01

    The subject of the research was the analysis of frequency and type of psychoactive substances used, basing on the determinations the blood and/or urine samples, performed in the toxicological laboratory of the Department of Clinical and Industrial Toxicology Jagiellonian University in Kraków in the period from December 2001 to November 2003. From 17,649 performed determinations--45.5% were positive. 50% of the positive determinations were psychoactive substances. The most often psychoactive substance determined was ethyl alcohol (52.86%), next benzodiazepines (17.41%), amphetamines (10.54%), opiates (8.05%), THC (6.87%), barbiturates (3.74%), and occasionally atropine and cocaine. There was observed a variety of mixed, simultaneously taking psychoactive substances, especially ethyl alcohol, opiates, amphetamine derivatives and cannabinoids. The analysis of the occurrence of psychoactive substances in biological samples from patients treated in different hospital departments, others hospitals and ordered by private persons also was performed. In the last two years 369 private patients ordered psychoactive substances determinations and 78 of them were positive.

  19. Laboratory technology research: Abstracts of FY 1998 projects

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-11-01

    The Laboratory Technology Research (LTR) program supports high-risk, multidisciplinary research partnerships to investigate challenging scientific problems whose solutions have promising commercial potential. These partnerships capitalize on two great strengths of the country: the world-class basic research capability of the DOE Office of Science (SC) national laboratories and the unparalleled entrepreneurial spirit of American industry. Projects supported by the LTR program in FY 1998 explore the applications of basic research advances relevant to DOE`s mission over a full range of scientific disciplines. The program presently emphasizes three critical areas of mission-related research: advanced materials, intelligent processing and manufacturing research, and environmental and biomedical research. Abstracts for 85 projects are contained in this report.

  20. Overview of DOE Oil and Gas Field Laboratory Projects

    Science.gov (United States)

    Bromhal, G.; Ciferno, J.; Covatch, G.; Folio, E.; Melchert, E.; Ogunsola, O.; Renk, J., III; Vagnetti, R.

    2017-12-01

    America's abundant unconventional oil and natural gas (UOG) resources are critical components of our nation's energy portfolio. These resources need to be prudently developed to derive maximum benefits. In spite of the long history of hydraulic fracturing, the optimal number of fracturing stages during multi-stage fracture stimulation in horizontal wells is not known. In addition, there is the dire need of a comprehensive understanding of ways to improve the recovery of shale gas with little or no impacts on the environment. Research that seeks to expand our view of effective and environmentally sustainable ways to develop our nation's oil and natural gas resources can be done in the laboratory or at a computer; but, some experiments must be performed in a field setting. The Department of Energy (DOE) Field Lab Observatory projects are designed to address those research questions that must be studied in the field. The Department of Energy (DOE) is developing a suite of "field laboratory" test sites to carry out collaborative research that will help find ways of improving the recovery of energy resources as much as possible, with as little environmental impact as possible, from "unconventional" formations, such as shale and other low permeability rock formations. Currently there are three field laboratories in various stages of development and operation. Work is on-going at two of the sites: The Hydraulic Fracturing Test Site (HFTS) in the Permian Basin and the Marcellus Shale Energy and Environmental Lab (MSEEL) project in the Marcellus Shale Play. Agreement on the third site, the Utica Shale Energy and Environmental Lab (USEEL) project in the Utica Shale Play, was just recently finalized. Other field site opportunities may be forthcoming. This presentation will give an overview of the three field laboratory projects.

  1. Laboratory technology research - abstracts of FY 1997 projects

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-11-01

    The Laboratory Technology Research (LTR) program supports high-risk, multidisciplinary research partnerships to investigate challenging scientific problems whose solutions have promising commercial potential. These partnerships capitalize on two great strengths of this country: the world-class basic research capability of the DOE Energy Research (ER) multi-program national laboratories and the unparalleled entrepreneurial spirit of American industry. A distinguishing feature of the ER multi-program national laboratories is their ability to integrate broad areas of science and engineering in support of national research and development goals. The LTR program leverages this strength for the Nation`s benefit by fostering partnerships with US industry. The partners jointly bring technology research to a point where industry or the Department`s technology development programs can pursue final development and commercialization. Projects supported by the LTR program are conducted by the five ER multi-program laboratories. These projects explore the applications of basic research advances relevant to DOE`s mission over a full range of scientific disciplines. The program presently emphasizes three critical areas of mission-related research: advanced materials; intelligent processing/manufacturing research; and sustainable environments.

  2. Project Management Plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    1995-04-01

    The purpose of the Isotopes Facilities Deactivation Project (IFDP) is to place former isotopes production facilities at the Oak Ridge National Laboratory in a safe, stable, and environmentally sound condition suitable for an extended period of minimum surveillance and maintenance (S ampersand M) and as quickly and economically as possible. Implementation and completion of the deactivation project will further reduce the already small risks to the environment and to public safety and health. Furthermore, the project should result in significant S ampersand M cost savings in the future. The IFDP management plan has been prepared to document the project objectives, define organizational relationships and responsibilities, and outline the management control systems to be employed in the management of the project. The project has adopted a strategy to deactivate the simple facilities first, to reduce the scope of the project, and to gain experience before addressing more difficult facilities. A decision support system is being developed to identify those activities, that best promote the project mission and result in largest cost savings. The Work Plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory (Energy Systems 1994) defines the project schedule, the cost estimate, and the technical approach for the project

  3. Proposed Casey's Pond Improvement Project, Fermi National Accelerator Laboratory

    International Nuclear Information System (INIS)

    1995-05-01

    The U.S. Department of Energy (DOE) has prepared an Environmental Assessment (EA), evaluating the impacts associated with the proposed Casey's Pond Improvement Project at the Fermi National Accelerator Laboratory (Fermilab) in Batavia, Illinois. The improvement project would maximize the efficiency of the Fermilab Industrial Cooling Water (ICW) distribution system, which removes (via evaporation) the thermal load from experimental and other support equipment supporting the high energy physics program at Fermilab. The project would eliminate the risk of overheating during fixed target experiments, ensure that the Illinois Water Quality Standards are consistently achieved and provide needed additional water storage for fire protection. Based on the analysis in the EA, the DOE has determined that the proposed action does not constitute a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement is not required

  4. [Historic Development of Clinical Biology Laboratories in Luxembourg].

    Science.gov (United States)

    Wennig R; Humbel R-L

    2014-01-01

    After a short overview on the development of diagnostic tools in clinical biology at an international level from Antiquity towards today, a history of the clinical biology including public and private institutions in Luxembourg will be outlined.

  5. Semester-long inquiry-based molecular biology laboratory: Transcriptional regulation in yeast.

    Science.gov (United States)

    Oelkers, Peter M

    2017-03-04

    A single semester molecular biology laboratory has been developed in which students design and execute a project examining transcriptional regulation in Saccharomyces cerevisiae. Three weeks of planning are allocated to developing a hypothesis through literature searches and use of bioinformatics. Common experimental plans address a cell process and how three genes that encode for proteins involved in that process are transcriptionally regulated in response to changing environmental conditions. Planning includes designing oligonucleotides to amplify the putative promoters of the three genes of interest. After the PCR, each product is cloned proximal to β-galactosidase in a yeast reporter plasmid. Techniques used include agarose electrophoresis, extraction of DNA from agarose, plasmid purification from bacteria, restriction digestion, ligation, and bacterial transformation. This promoter/reporter plasmid is then transformed into yeast. Transformed yeast are cultured in conditions prescribed in the experimental design, lysed and β-galactosidase activity is measured. The course provides an independent research experience in a group setting. Notebooks are maintained on-line with regular feedback. Projects culminate with the presentation of a poster worth 60% of the grade. Over the last three years, about 65% of students met expectations for experimental design, data acquisition, and analysis. © 2016 by The International Union of Biochemistry and Molecular Biology, 45(2):145-151, 2017. © 2016 The International Union of Biochemistry and Molecular Biology.

  6. The Next Generation of Synthetic Biology Chassis: Moving Synthetic Biology from the Laboratory to the Field.

    Science.gov (United States)

    Adams, Bryn L

    2016-12-16

    Escherichia coli (E. coli) has played a pivotal role in the development of genetics and molecular biology as scientific fields. It is therefore not surprising that synthetic biology (SB) was built upon E. coli and continues to dominate the field. However, scientific capabilities have advanced from simple gene mutations to the insertion of rationally designed, complex synthetic circuits and creation of entirely synthetic genomes. The point is rapidly approaching where E. coli is no longer an adequate host for the increasingly sophisticated genetic designs of SB. It is time to develop the next generation of SB chassis; robust organisms that can provide the advanced physiology novel synthetic circuits will require to move SB from the laboratory into fieldable technologies. This can be accomplished by developing chassis-specific genetic toolkits that are as extensive as those for E. coli. However, the holy grail of SB would be the development of a universal toolkit that can be ported into any chassis. This viewpoint article underscores the need for new bacterial chassis, as well as discusses some of the important considerations in their selection. It also highlights a few examples of robust, tractable bacterial species that can meet the demands of tomorrow's state-of-the-art in SB. Significant advances have been made in the first 15 years since this field has emerged. However, the advances over the next 15 years will occur not in laboratory organisms, but in fieldable species where the potential of SB can be fully realized in game changing technology.

  7. Cursory radiological assessment: Battelle Columbus Laboratory Decommissioning and Decontamination Project

    International Nuclear Information System (INIS)

    Smith, W.H.; Munyon, W.J.; Mosho, G.D.; Robinet, M.J.; Wynveen, R.A.

    1988-10-01

    This document reports on the results obtained from a cursory radiological assessment of various properties at the Battelle Columbus Laboratory, Columbia, Ohio. The cursory radiological assessment is part of a preliminary investigation for the Battelle Columbus Laboratory Decommissioning and Decontamination Project. The radiological assessment of Battelle Columbus Laboratory's two sites included conducting interior and exterior building surveys and collecting and analyzing air, sewer system, and soil samples. Direct radiological surveys were made of floor, wall, and overhead areas. Smear surveys were made on various interior building surfaces as well as the exterior building vents. Air samples were collected in select areas to determine concentrations of Rn-222, Rn-220, and Rn-219 daughters, in addition to any long-lived radioactive particulates. Radon-222 concentrations were continuously monitored over a 24-hr period at several building locations using a radon gas monitoring system. The sanitary sewer systems at King Avenue, West Jefferson-North, and West Jefferson-South were each sampled at select locations. All samples were submitted to the Argonne Analytical Chemistry Laboratory for various radiological and chemical analyses. Environmental soil corings were taken at both the King Avenue and West Jefferson sites to investigate the potential for soil contamination within the first 12-inches below grade. Further subsurface investigations at the West Jefferson-North and West Jefferson-South areas were conducted using soil boring techniques. 4 refs., 10 figs., 10 tabs

  8. Idaho National Laboratory Annual Report FY 2013 LDRD Project Summaries

    Energy Technology Data Exchange (ETDEWEB)

    Dena Tomchak

    2014-03-01

    The FY 2013 LDRD Annual Report is a compendium of the diverse research performed to develop and ensure the INL’s technical capabilities support the current and future DOE missions and national research priorities. LDRD is essential to INL—it provides a means for the Laboratory to maintain scientific and technical vitality while funding highly innovative, high-risk science and technology research and development (R&D) projects. The program enhances technical capabilities at the Laboratory, providing scientific and engineering staff with opportunities to explore proof-of-principle ideas, advanced studies of innovative concepts, and preliminary technical analyses. Established by Congress in 1991, the LDRD Program proves its benefit each year through new programs, intellectual property, patents, copyrights, national and international awards, and publications.

  9. [Non-conformities management in laboratory of medical biology: application to non-conformities of biological samples during 2009].

    Science.gov (United States)

    Annaix, Véronique; Rogowski, Julien; Joyau, Mireille; Jaouën, Edtih

    2011-01-01

    The non-conformity management is required for the ISO 15189 standard. The laboratory of medical biology has to carry out suitable acts and procedures to exploit different indicators through the framework of continuous improvement. We particularly study the indicator of biological samples nonconformities and we report 2009 results to the nurses' team managers to find solutions for quality of care to the patient.

  10. The Los Alamos National Laboratory Transuranic Waste Retireval Project

    International Nuclear Information System (INIS)

    Montoya, G.M.; Christensen, D.V.; Stanford, A.R.

    1997-01-01

    This paper presents the status of the Los Alamos National Laboratory (LANL) project for remediation of transuranic (TRU) and TRU mixed waste from Pads 1, 2, and 4. Some of the TRU waste packages retrieved from Pad I are anticipated to be part of LANL's initial inventory to be shipped to the Waste Isolation Pilot Plant (WIPP) in April 1998. The TRU Waste Inspectable Storage Project (TWISP) was initiated in February 1993 in response to the New Mexico Environment Department's (NMED's) Consent Agreement for Compliance Order, ''New Mexico Hazardous Waste Agreement (NMHWA) 93-03.'' The TWISP involves the recovery of approximately 16,865 TRU and TRU-mixed waste containers currently under earthen cover on Pads 1, 2, and 4 at Technical Area 54, Area G, and placement of that waste into inspectable storage. All waste will be moved into inspectable storage by September 30, 2003. Waste recovery and storage operations emphasize protection of worker safety, public health, and the environment

  11. Los Alamos National Laboratory TRU waste sampling projects

    International Nuclear Information System (INIS)

    Yeamans, D.; Rogers, P.; Mroz, E.

    1997-01-01

    The Los Alamos National Laboratory (LANL) has begun characterizing transuranic (TRU) waste in order to comply with New Mexico regulations, and to prepare the waste for shipment and disposal at the Waste Isolation Pilot Plant (WIPP), near Carlsbad, New Mexico. Sampling consists of removing some head space gas from each drum, removing a core from a few drums of each homogeneous waste stream, and visually characterizing a few drums from each heterogeneous waste stream. The gases are analyzed by GC/MS, and the cores are analyzed for VOC's and SVOC's by GC/MS and for metals by AA or AE spectroscopy. The sampling and examination projects are conducted in accordance with the ''DOE TRU Waste Quality Assurance Program Plan'' (QAPP) and the ''LANL TRU Waste Quality Assurance Project Plan,'' (QAPjP), guaranteeing that the data meet the needs of both the Carlsbad Area Office (CAO) of DOE and the ''WIPP Waste Acceptance Criteria, Rev. 5,'' (WAC)

  12. The Human Genome Project: big science transforms biology and medicine

    OpenAIRE

    Hood, Leroy; Rowen, Lee

    2013-01-01

    The Human Genome Project has transformed biology through its integrated big science approach to deciphering a reference human genome sequence along with the complete sequences of key model organisms. The project exemplifies the power, necessity and success of large, integrated, cross-disciplinary efforts - so-called ‘big science’ - directed towards complex major objectives. In this article, we discuss the ways in which this ambitious endeavor led to the development of novel technologies and a...

  13. The Advanced Interdisciplinary Research Laboratory: A Student Team Approach to the Fourth-Year Research Thesis Project Experience

    Science.gov (United States)

    Piunno, Paul A. E.; Boyd, Cleo; Barzda, Virginijus; Gradinaru, Claudiu C.; Krull, Ulrich J.; Stefanovic, Sasa; Stewart, Bryan

    2014-01-01

    The advanced interdisciplinary research laboratory (AIRLab) represents a novel, effective, and motivational course designed from the interdisciplinary research interests of chemistry, physics, biology, and education development faculty members as an alternative to the independent thesis project experience. Student teams are assembled to work…

  14. 78 FR 32637 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2013-05-31

    ..., Science and Technology Reinvention Laboratory Personnel Management Demonstration Project, Department of... DEPARTMENT OF DEFENSE Office of the Secretary Science and Technology Reinvention Laboratory Personnel Management Demonstration Project, Department of the Army, Army Research, Development and...

  15. Aespoe hard rock laboratory. Current research projects 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    In 1986 SKB decided to construct the Aespoe Hard Rock Laboratory (HRL) in order to provide an opportunity for research, development and demonstration in a realistic and undisturbed underground rock environment down to the depth planned for the future deep repository. The focus of current and future work is on development and testing of site characterization methods, verification of models describing the function of the natural and engineered barriers and development, testing, and demonstration of repository technology. The program has been organised so that all important steps in the development of a repository are covered, in other words the Aespoe HRL constitutes a `dress rehearsal` for the Swedish deep geological repository for spent fuel and other long-lived waste. Geoscientific investigations on Aespoe and nearby islands began in 1986. Aespoe was selected as the site for the laboratory in 1988. Construction of the facility, which reaches a depth of 460 m below the surface, began in 1990 and was completed in 1995. A major milestone had been reached in 1996 with the completion of the pre-investigation and construction phases of the Aespoe HRL. The comprehensive research conducted has permitted valuable development and verification of site characterization methods applied from the ground surface, boreholes, and underground excavations. The results of this research are summarised in the book `Aespoe Hard Rock Laboratory - 10 years of Research` published by SKB in 1996. The Operating Phase of the Aespoe HRL began in 1995 and is expected to continue for 15-20 years, that is until the first stage of the development of the Swedish deep geological repository for spent nuclear fuel is expected to be completed. A number of research projects were initiated at the start of the Operating Phase. Most of these projects have made substantial progress since then and important results have been obtained. The purpose of this brochure is to provide a brief presentation of the

  16. Aespoe hard rock laboratory. Current research projects 1998

    International Nuclear Information System (INIS)

    1998-01-01

    In 1986 SKB decided to construct the Aespoe Hard Rock Laboratory (HRL) in order to provide an opportunity for research, development and demonstration in a realistic and undisturbed underground rock environment down to the depth planned for the future deep repository. The focus of current and future work is on development and testing of site characterization methods, verification of models describing the function of the natural and engineered barriers and development, testing, and demonstration of repository technology. The program has been organised so that all important steps in the development of a repository are covered, in other words the Aespoe HRL constitutes a 'dress rehearsal' for the Swedish deep geological repository for spent fuel and other long-lived waste. Geoscientific investigations on Aespoe and nearby islands began in 1986. Aespoe was selected as the site for the laboratory in 1988. Construction of the facility, which reaches a depth of 460 m below the surface, began in 1990 and was completed in 1995. A major milestone had been reached in 1996 with the completion of the pre-investigation and construction phases of the Aespoe HRL. The comprehensive research conducted has permitted valuable development and verification of site characterization methods applied from the ground surface, boreholes, and underground excavations. The results of this research are summarised in the book 'Aespoe Hard Rock Laboratory - 10 years of Research' published by SKB in 1996. The Operating Phase of the Aespoe HRL began in 1995 and is expected to continue for 15-20 years, that is until the first stage of the development of the Swedish deep geological repository for spent nuclear fuel is expected to be completed. A number of research projects were initiated at the start of the Operating Phase. Most of these projects have made substantial progress since then and important results have been obtained. The purpose of this brochure is to provide a brief presentation of the

  17. CNR LARA project, Italy: Airborne laboratory for environmental research

    Science.gov (United States)

    Bianchi, R.; Cavalli, R. M.; Fiumi, L.; Marino, C. M.; Pignatti, S.

    1995-01-01

    The increasing interest for the environmental problems and the study of the impact on the environment due to antropic activity produced an enhancement of remote sensing applications. The Italian National Research Council (CNR) established a new laboratory for airborne hyperspectral imaging, the LARA Project (Laboratorio Aero per Ricerche Ambientali - Airborne Laboratory for Environmental Research), equipping its airborne laboratory, a CASA-212, mainly with the Daedalus AA5000 MIVIS (Multispectral Infrared and Visible Imaging Spectrometer) instrument. MIVIS's channels, spectral bandwidths, and locations are chosen to meet the needs of scientific research for advanced applications of remote sensing data. MIVIS can make significant contributions to solving problems in many diverse areas such as geologic exploration, land use studies, mineralogy, agricultural crop studies, energy loss analysis, pollution assessment, volcanology, forest fire management and others. The broad spectral range and the many discrete narrow channels of MIVIS provide a fine quantization of spectral information that permits accurate definition of absorption features from a variety of materials, allowing the extraction of chemical and physical information of our environment. The availability of such a hyperspectral imager, that will operate mainly in the Mediterranean area, at the present represents a unique opportunity for those who are involved in environmental studies and land-management to collect systematically large-scale and high spectral-spatial resolution data of this part of the world. Nevertheless, MIVIS deployments will touch other parts of the world, where a major interest from the international scientific community is present.

  18. A Project-Based Biologically-Inspired Robotics Module

    Science.gov (United States)

    Crowder, R. M.; Zauner, K.-P.

    2013-01-01

    The design of any robotic system requires input from engineers from a variety of technical fields. This paper describes a project-based module, "Biologically-Inspired Robotics," that is offered to Electronics and Computer Science students at the University of Southampton, U.K. The overall objective of the module is for student groups to…

  19. The Human Genome Project: Biology, Computers, and Privacy.

    Science.gov (United States)

    Cutter, Mary Ann G.; Drexler, Edward; Gottesman, Kay S.; Goulding, Philip G.; McCullough, Laurence B.; McInerney, Joseph D.; Micikas, Lynda B.; Mural, Richard J.; Murray, Jeffrey C.; Zola, John

    This module, for high school teachers, is the second of two modules about the Human Genome Project (HGP) produced by the Biological Sciences Curriculum Study (BSCS). The first section of this module provides background information for teachers about the structure and objectives of the HGP, aspects of the science and technology that underlie the…

  20. Enhancing Biology Instruction with the Human Genome Project

    Science.gov (United States)

    Buxeda, Rosa J.; Moore-Russo, Deborah A.

    2003-01-01

    The Human Genome Project (HGP) is a recent scientific milestone that has received notable attention. This article shows how a biology course is using the HGP to enhance students' experiences by providing awareness of cutting edge research, with information on new emerging career options, and with opportunities to consider ethical questions raised…

  1. Development and Assessment of Service Learning Projects in General Biology

    Science.gov (United States)

    Felzien, Lisa; Salem, Laura

    2008-01-01

    Service learning involves providing service to the community while requiring students to meet learning goals in a specific course. A service learning project was implemented in a general biology course at Rockhurst University to involve students in promoting scientific education in conjunction with community partner educators. Students were…

  2. The Human Genome Project: big science transforms biology and medicine.

    Science.gov (United States)

    Hood, Leroy; Rowen, Lee

    2013-01-01

    The Human Genome Project has transformed biology through its integrated big science approach to deciphering a reference human genome sequence along with the complete sequences of key model organisms. The project exemplifies the power, necessity and success of large, integrated, cross-disciplinary efforts - so-called 'big science' - directed towards complex major objectives. In this article, we discuss the ways in which this ambitious endeavor led to the development of novel technologies and analytical tools, and how it brought the expertise of engineers, computer scientists and mathematicians together with biologists. It established an open approach to data sharing and open-source software, thereby making the data resulting from the project accessible to all. The genome sequences of microbes, plants and animals have revolutionized many fields of science, including microbiology, virology, infectious disease and plant biology. Moreover, deeper knowledge of human sequence variation has begun to alter the practice of medicine. The Human Genome Project has inspired subsequent large-scale data acquisition initiatives such as the International HapMap Project, 1000 Genomes, and The Cancer Genome Atlas, as well as the recently announced Human Brain Project and the emerging Human Proteome Project.

  3. 2017 GTO Project review Laboratory Evaluation of EGS Shear Stimulation.

    Energy Technology Data Exchange (ETDEWEB)

    Bauer, Stephen J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-10-01

    The objectives and purpose of this research has been to produce laboratory-based experimental and numerical analyses to provide a physics-based understanding of shear stimulation phenomena (hydroshearing) and its evolution during stimulation. Water was flowed along fractures in hot and stressed fractured rock, to promote slip. The controlled laboratory experiments provide a high resolution/high quality data resource for evaluation of analysis methods developed by DOE to assess EGS “behavior” during this stimulation process. Segments of the experimental program will provide data sets for model input parameters, i.e., material properties, and other segments of the experimental program will represent small scale physical models of an EGS system, which may be modeled. The coupled lab/analysis project has been a study of the response of a fracture in hot, water-saturated fractured rock to shear stress experiencing fluid flow. Under this condition, the fracture experiences a combination of potential pore pressure changes and fracture surface cooling, resulting in slip along the fracture. The laboratory work provides a means to assess the role of “hydroshearing” on permeability enhancement in reservoir stimulation. Using the laboratory experiments and results to define boundary and input/output conditions of pore pressure, thermal stress, fracture shear deformation and fluid flow, and models were developed and simulations completed by the University of Oklahoma team. The analysis methods are ones used on field scale problems. The sophisticated numerical models developed contain parameters present in the field. The analysis results provide insight into the role of fracture slip on permeability enhancement-“hydroshear” is to be obtained. The work will provide valuable input data to evaluate stimulation models, thus helping design effective EGS.

  4. Enhancing Scientific Inquiry Literacy of Prospective Biology Teachers through Inquiry Lab Project in Microbiology

    Science.gov (United States)

    Kusnadi, K.; Rustaman, N. Y.; Redjeki, S.; Aryantha, I. N. P.

    2017-09-01

    The implementation of the inquiry laboratory based project to enhance scientific inquiry literacy of prospective biology teachers in Microbiology course has been done. The inquiry lab based project was designed by three stages were debriefing of basic microbiology lab skills, guided inquiry and free inquiry respectively. The Study was quasi experimental with control group pretest-posttest design. The subjects were prospective biology teachers consists of 80 students. The scientific inquiry literacy instrument refers to ScInqLiT by Wenning. The results showed that there was significant difference of scientific inquiry literacy posttest scores between experiment and control (α 0,05) and was obtained N-gain score was 0.49 (medium) to experiment and 0.24 (low) to control. Based on formative assessment showed that development of student’s scientific attitude, research and microbiology lab skills during conducting project were increased. Student’s research skills especially in identification of variables, constructing a hypothesis, communicating and concluding were increased. During implementation of inquiry project also showed that they carried out mind and hands-on and so collaborative group investigation lab activities. Our findings may aid in reforming higher-education, particularly in microbiology laboratory activities to better promote scientific inquiry literacy, scientific attitude, research and laboratory skills.

  5. Research Projects for Interrogations of Biological Systems: Training for the Development of Novel Radiotracers

    Energy Technology Data Exchange (ETDEWEB)

    Jurisson, Silvia S. [Univ. of Missouri, Columbia, MO (United States); Lever, Susan Z. [Univ. of Missouri, Columbia, MO (United States); Robertson, J. David [Univ. of Missouri, Columbia, MO (United States)

    2016-10-04

    This grant was situated at the University of Missouri to train Ph.D. scientists in radiochemistry and synthetic chemistry in conjunction with Faculty from the Interdisciplinary Plant Group, Division of Biological Sciences, the MU Research Reactor Center, Molecular Biology and the Radiopharmaceutical Sciences Institute. This project was collaborative with Brookhaven National Laboratory (Richard Ferrieri, PI). Projects for the Ph.D. candidates included novel probe development for peptides, nucleosides, small molecules or radiometals, the direct use of radiometals as probes, or nuclear techniques for analysis. The projects for the postdoctoral fellow involved synthetic chemistry for the preparation of precursors for novel tracers that will be radiolabeled with 18F or other appropriate radionuclides. The skill sets of our team members allowed us to prepare probes with positron or single photon emitters, as well as ones that are dual-labeled (fluorescent and radiolabeled). We focused our technical advances to those that will be broadly applicable to any research field.

  6. Research Projects for Interrogations of Biological Systems: Training for the Development of Novel Radiotracers

    International Nuclear Information System (INIS)

    Jurisson, Silvia S.; Lever, Susan Z.; Robertson, J. David

    2016-01-01

    This grant was situated at the University of Missouri to train Ph.D. scientists in radiochemistry and synthetic chemistry in conjunction with Faculty from the Interdisciplinary Plant Group, Division of Biological Sciences, the MU Research Reactor Center, Molecular Biology and the Radiopharmaceutical Sciences Institute. This project was collaborative with Brookhaven National Laboratory (Richard Ferrieri, PI). Projects for the Ph.D. candidates included novel probe development for peptides, nucleosides, small molecules or radiometals, the direct use of radiometals as probes, or nuclear techniques for analysis. The projects for the postdoctoral fellow involved synthetic chemistry for the preparation of precursors for novel tracers that will be radiolabeled with "1"8F or other appropriate radionuclides. The skill sets of our team members allowed us to prepare probes with positron or single photon emitters, as well as ones that are dual-labeled (fluorescent and radiolabeled). We focused our technical advances to those that will be broadly applicable to any research field.

  7. Team research at the biology-mathematics interface: project management perspectives.

    Science.gov (United States)

    Milton, John G; Radunskaya, Ami E; Lee, Arthur H; de Pillis, Lisette G; Bartlett, Diana F

    2010-01-01

    The success of interdisciplinary research teams depends largely upon skills related to team performance. We evaluated student and team performance for undergraduate biology and mathematics students who participated in summer research projects conducted in off-campus laboratories. The student teams were composed of a student with a mathematics background and an experimentally oriented biology student. The team mentors typically ranked the students' performance very good to excellent over a range of attributes that included creativity and ability to conduct independent research. However, the research teams experienced problems meeting prespecified deadlines due to poor time and project management skills. Because time and project management skills can be readily taught and moreover typically reflect good research practices, simple modifications should be made to undergraduate curricula so that the promise of initiatives, such as MATH-BIO 2010, can be implemented.

  8. Lessons learned from decommissioning projects at Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Salazar, M.

    1995-01-01

    This paper describes lessons learned over the last 20 years from 12 decommissioning projects at Los Alamos National Laboratory. These lessons relate both to overall program management and to management of specific projects during the planning and operations phases. The issues include waste management; the National Environmental Policy Act (NEPA); the Resource Conservation and Recovery Act (RCRA); the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA); contracting; public involvement; client/customer interface; and funding. Key elements of our approach are to be proactive; follow the observation method; perform field activities concurrently; develop strategies to keep reportable incidents from delaying work; seek and use programs, methods, etc., in existence to shorten learning curves; network to help develop solutions; and avoid overstudying and overcharacterizing. This approach results in preliminary plans that require very little revision before implementation, reasonable costs and schedules, early acquisition of permits and NEPA documents, preliminary characterization reports, and contracting documents. Our track record is good -- the last four projects (uranium and plutonium-processing facility and three research reactors) have been on budget and on schedule

  9. Biological Assessment of the Continued Operation of Los Alamos National Laboratory on Federally Listed Threatened and Endangered Species

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, Leslie A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Ecology and Air Quality Group

    2006-09-19

    This biological assessment considers the effects of continuing to operate Los Alamos National Laboratory on Federally listed threatened or endangered species, based on current and future operations identified in the 2006 Site-wide Environmental Impact Statement for the Continued Operation of Los Alamos National Laboratory (SWEIS; DOE In Prep.). We reviewed 40 projects analyzed in the SWEIS as well as two aspects on ongoing operations to determine if these actions had the potential to affect Federally listed species. Eighteen projects that had not already received U.S. Fish and Wildlife Service (USFWS) consultation and concurrence, as well as the two aspects of ongoing operations, ecological risk from legacy contaminants and the Outfall Reduction Project, were determined to have the potential to affect threatened or endangered species. Cumulative impacts were also analyzed.

  10. Interlaboratory control among INCO-DEV MYCOTOX PROJECT LABORATORIES

    Directory of Open Access Journals (Sweden)

    E.A Vargas

    2011-04-01

    Full Text Available The Work Package 1 “ Development and standardization of effective analytical tools for mycotoxin (aflatoxins B1, B2  G1, G2  ochratoxin A, zearalenone, fumonisin B1, B2  and tricothecenes determination in wheat and maize”  aim to implement the interlaboratory control between the partners laboratories from Brazil, Uruguay, Chile and Argentina as part of the objectives of INCO-DEV MYCOTOX PROJECT 2003-2005  “The Development of a Food Quality Management System for the Control of Mycotoxins in cereal Production and Processing Chains in Latin America South Cone Countries”.  The ojectives of the interlaboratory control were: evaluate the performance of the laboratories and the main difficulties encountered in performing the analytical procedure for mycotoxins  determination in maize and wheat; contribute to the harmonization of analytical procedures of the partners laboratories and contribute to the laboratory’s proficiency in mycotoxin analysis.  Maize reference materials for aflatoxins and zearealenone were prepared and used to the implementation of the interlaboratory control.  In summary, the preparation of these samples involved: milling (<20 mesh, homogeneization, analysis to verify the homogeneity of the bulk material and packing (labelled vacuum “sachets” or plastic bottles and mycotoxin analysis.  The homogeneity of the material was investigated by the analysis of variance – ANOVA- according to International Harmonized Protocol for the Proficiency testing of (ChemicalAnalytical Laboratories as established by ISO 43-1 – Annex at 95% of confidence level by calculating an F-statistic ans Ss/ÿ (ÿ =15%. All batches of test material were stored under – 18ºC and protected from light prior to and after packaging.  Aflatoxins in the test materials were determinated by immunoaffinity with liquid chromatography (LC with pos-column derivatization and thin layer chromatography (TLC.  Zearalenone in the test materials

  11. Laboratory procedures used in the hot corrosion project

    International Nuclear Information System (INIS)

    Jeys, T.R.

    1980-01-01

    The objective of the Hot Corrosion Project in the LLNL Metals and Ceramics Division is to study the physical and chemical mechanisms of corrosion of nickel, iron, and some of their alloys when these metals are subjected to oxidizing or sulfidizing environments at temperatures between 850 and 950 0 C. To obtain meaningful data in this study, we must rigidly control many parameters. Parameters are discussed and the methods chosen to control them in this laboratory. Some of the mechanics and manipulative procedures that are specifically related to data access and repeatability are covered. The method of recording and processing the data from each experiment using an LS-11 minicomputer are described. The analytical procedures used to evaluate the specimens after the corrosion tests are enumerated and discussed

  12. CERTS Microgrid Laboratory Test Bed - PIER Final Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Eto, Joseph H.; Eto, Joseph H.; Lasseter, Robert; Schenkman, Ben; Klapp, Dave; Linton, Ed; Hurtado, Hector; Roy, Jean; Lewis, Nancy Jo; Stevens, John; Volkommer, Harry

    2008-07-25

    The objective of the CERTS Microgrid Laboratory Test Bed project was to enhance the ease of integrating small energy sources into a microgrid. The project accomplished this objective by developing and demonstrating three advanced techniques, collectively referred to as the CERTS Microgrid concept, that significantly reduce the level of custom field engineering needed to operate microgrids consisting of small generating sources. The techniques comprising the CERTS Microgrid concept are: 1) a method for effecting automatic and seamless transitions between grid-connected and islanded modes of operation; 2) an approach to electrical protection within the microgrid that does not depend on high fault currents; and 3) a method for microgrid control that achieves voltage and frequency stability under islanded conditions without requiring high-speed communications. The techniques were demonstrated at a full-scale test bed built near Columbus, Ohio and operated by American Electric Power. The testing fully confirmed earlier research that had been conducted initially through analytical simulations, then through laboratory emulations, and finally through factory acceptance testing of individual microgrid components. The islanding and resychronization method met all Institute of Electrical and Electronics Engineers 1547 and power quality requirements. The electrical protections system was able to distinguish between normal and faulted operation. The controls were found to be robust and under all conditions, including difficult motor starts. The results from these test are expected to lead to additional testing of enhancements to the basic techniques at the test bed to improve the business case for microgrid technologies, as well to field demonstrations involving microgrids that involve one or mroe of the CERTS Microgrid concepts.

  13. Summaries of the Idaho National Engineering Laboratory Radioecology and Ecology Program research projects

    International Nuclear Information System (INIS)

    Markham, O.D.

    1987-06-01

    This report provides summaries of individual research projects conducted by the Idaho National Engineering Laboratory Radioecology and Ecology Program. Summaries include projects in various stages, from those that are just beginning, to projects that are in the final publication stage

  14. Mizunami Underground Research Laboratory project. Plan for fiscal year 2017

    International Nuclear Information System (INIS)

    Ishibashi, Masayuki; Hama, Katsuhiro; Iwatsuki, Teruki; Matsui, Hiroya; Takeuchi, Ryuji; Ikeda, Koki; Mikake, Shinichiro; Iyatomi, Yosuke; Sasao, Eiji; Koide, Kaoru

    2017-10-01

    The Mizunami Underground Research Laboratory (MIU) Project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of geological disposal technologies through investigations of the deep geological environment in the crystalline host rock (granite) at Mizunami, Gifu Prefecture, central Japan. On the occasion of the reform of the entire JAEA organization in 2014, JAEA identified three important issues on the geoscientific research program: 'Development of countermeasure technologies for reducing groundwater inflow', 'Development of modelling technologies for mass transport' and 'Development of drift backfilling technology', based on the latest results of the synthesizing research and development (R and D). The R and D on three remaining important issues has been carrying out on the MIU Project. This report summarizes the R and D activities planned for fiscal year 2017 on the basis of the MIU Master Plan updated in 2015 and Investigation Plan for the Third Medium to Long-term Research Phase. (author)

  15. Prototype Biology-Based Radiation Risk Module Project

    Science.gov (United States)

    Terrier, Douglas; Clayton, Ronald G.; Patel, Zarana; Hu, Shaowen; Huff, Janice

    2015-01-01

    Biological effects of space radiation and risk mitigation are strategic knowledge gaps for the Evolvable Mars Campaign. The current epidemiology-based NASA Space Cancer Risk (NSCR) model contains large uncertainties (HAT #6.5a) due to lack of information on the radiobiology of galactic cosmic rays (GCR) and lack of human data. The use of experimental models that most accurately replicate the response of human tissues is critical for precision in risk projections. Our proposed study will compare DNA damage, histological, and cell kinetic parameters after irradiation in normal 2D human cells versus 3D tissue models, and it will use a multi-scale computational model (CHASTE) to investigate various biological processes that may contribute to carcinogenesis, including radiation-induced cellular signaling pathways. This cross-disciplinary work, with biological validation of an evolvable mathematical computational model, will help reduce uncertainties within NSCR and aid risk mitigation for radiation-induced carcinogenesis.

  16. Mouse Genome Informatics (MGI) Resource: Genetic, Genomic, and Biological Knowledgebase for the Laboratory Mouse.

    Science.gov (United States)

    Eppig, Janan T

    2017-07-01

    The Mouse Genome Informatics (MGI) Resource supports basic, translational, and computational research by providing high-quality, integrated data on the genetics, genomics, and biology of the laboratory mouse. MGI serves a strategic role for the scientific community in facilitating biomedical, experimental, and computational studies investigating the genetics and processes of diseases and enabling the development and testing of new disease models and therapeutic interventions. This review describes the nexus of the body of growing genetic and biological data and the advances in computer technology in the late 1980s, including the World Wide Web, that together launched the beginnings of MGI. MGI develops and maintains a gold-standard resource that reflects the current state of knowledge, provides semantic and contextual data integration that fosters hypothesis testing, continually develops new and improved tools for searching and analysis, and partners with the scientific community to assure research data needs are met. Here we describe one slice of MGI relating to the development of community-wide large-scale mutagenesis and phenotyping projects and introduce ways to access and use these MGI data. References and links to additional MGI aspects are provided. © The Author 2017. Published by Oxford University Press.

  17. Student Perceptions of the Cell Biology Laboratory Learning Environment in Four Undergraduate Science Courses in Spain

    Science.gov (United States)

    De Juan, Joaquin; Pérez-Cañaveras, Rosa M.; Segovia, Yolanda; Girela, Jose Luis; Martínez-Ruiz, Noemi; Romero-Rameta, Alejandro; Gómez-Torres, Maria José; Vizcaya-Moreno, M. Flores

    2016-01-01

    Cell biology is an academic discipline that organises and coordinates the learning of the structure, function and molecular composition of cells in some undergraduate biomedical programs. Besides course content and teaching methodologies, the laboratory environment is considered a key element in the teaching of and learning of cell biology. The…

  18. Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory.

    Science.gov (United States)

    Bowey-Dellinger, Kristen; Dixon, Luke; Ackerman, Kristin; Vigueira, Cynthia; Suh, Yewseok K; Lyda, Todd; Sapp, Kelli; Grider, Michael; Crater, Dinene; Russell, Travis; Elias, Michael; Coffield, V McNeil; Segarra, Verónica A

    2017-01-01

    Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques. For this reason, we have devised a laboratory module and method herein that familiarizes students with common cell culture procedures, without the use of a sterile hood or large cell culture facility. Students design and perform a time-efficient inquiry-based cell viability experiment using HeLa cells and tools that are readily available in an undergraduate biology laboratory. Students will become familiar with common techniques such as trypsinizing cells, cell counting with a hemocytometer, performing serial dilutions, and determining cell viability using trypan blue dye. Additionally, students will work with graphing software to analyze their data and think critically about the mechanism of death on a cellular level. Two different adaptations of this inquiry-based lab are presented-one for non-biology majors and one for biology majors. Overall, these laboratories aim to expose students to mammalian cell culture and basic techniques and help them to conceptualize their application in scientific research.

  19. Fruit Flies Provide New Insights in Low-Radiation Background Biology at the INFN Underground Gran Sasso National Laboratory (LNGS).

    Science.gov (United States)

    Morciano, Patrizia; Cipressa, Francesca; Porrazzo, Antonella; Esposito, Giuseppe; Tabocchini, Maria Antonella; Cenci, Giovanni

    2018-06-04

    Deep underground laboratories (DULs) were originally created to host particle, astroparticle or nuclear physics experiments requiring a low-background environment with vastly reduced levels of cosmic-ray particle interference. More recently, the range of science projects requiring an underground experiment site has greatly expanded, thus leading to the recognition of DULs as truly multidisciplinary science sites that host important studies in several fields, including geology, geophysics, climate and environmental sciences, technology/instrumentation development and biology. So far, underground biology experiments are ongoing or planned in a few of the currently operating DULs. Among these DULs is the Gran Sasso National Laboratory (LNGS), where the majority of radiobiological data have been collected. Here we provide a summary of the current scenario of DULs around the world, as well as the specific features of the LNGS and a summary of the results we obtained so far, together with other findings collected in different underground laboratories. In particular, we focus on the recent results from our studies of Drosophila melanogaster, which provide the first evidence of the influence of the radiation environment on life span, fertility and response to genotoxic stress at the organism level. Given the increasing interest in this field and the establishment of new projects, it is possible that in the near future more DULs will serve as sites of radiobiology experiments, thus providing further relevant biological information at extremely low-dose-rate radiation. Underground experiments can be nicely complemented with above-ground studies at increasing dose rate. A systematic study performed in different exposure scenarios provides a potential opportunity to address important radiation protection questions, such as the dose/dose-rate relationship for cancer and non-cancer risk, the possible existence of dose/dose-rate threshold(s) for different biological systems and

  20. Project and implementation of the human/system interface laboratory

    International Nuclear Information System (INIS)

    Carvalho, Paulo Victor R. de; Obadia, Isaac Jose; Vidal, Mario Cesar Rodriguez

    2002-01-01

    Analog instrumentation is being increasingly replaced by digital technology in new nuclear power plants, such as Angra III, as well as in existing operating plants, such as Angra I and II, for modernization and life-extension projects. In this new technological environment human factors issues aims to minimize failures in nuclear power plants operation due to human error. It is well known that 30% to 50% of the detected unforeseen problems involve human errors. Presently, human factors issues must be considered during the development of advanced human-system interfaces for the plant. IAEA has considered the importance of those issues and has published TECDOC's and Safety Series Issues on the matter. Thus, there is a need to develop methods and criteria to asses, compare, optimize and validate the human-system interface associated with totally new or hybrid control rooms. Also, the use of computer based operator aids is en evolving area. In order to assist on the development of methods and criteria and to evaluate the effects of the new design concepts and computerized support systems on operator performance, research simulators with advanced control rooms technology, such the IEN's Human System Interface Laboratory, will provide the necessary setting. (author)

  1. Isotopes facilities deactivation project at Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Eversole, R.E.

    1997-05-01

    The production and distribution of radioisotopes for medical, scientific, and industrial applications has been a major activity at Oak Ridge National Laboratory (ORNL) since the late 1940s. As the demand for many of these isotopes grew and their sale became profitable, the technology for the production of the isotopes was transferred to private industry, and thus, many of the production facilities at ORNL became underutilized. In 1989, the U.S. Department of Energy (DOE) instructed ORNL to identify and prepare various isotopes production facilities for safe shutdown. In response, ORNL identified 19 candidate facilities for shutdown and established the Isotopes Facilities Shutdown Program. In 1993, responsibility for the program was transitioned from the DOE Office of Nuclear Energy to the DOE Office of Environmental Management and Uranium Enrichment Operation`s Office of Facility Transition and Management. The program was retitled the Isotopes Facilities Deactivation Project (IFDP), and implementation responsibility was transferred from ORNL to the Lockheed Martin Energy Systems, Inc. (LMES), Environmental Restoration (ER) Program.

  2. Isotopes facilities deactivation project at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Eversole, R.E.

    1997-01-01

    The production and distribution of radioisotopes for medical, scientific, and industrial applications has been a major activity at Oak Ridge National Laboratory (ORNL) since the late 1940s. As the demand for many of these isotopes grew and their sale became profitable, the technology for the production of the isotopes was transferred to private industry, and thus, many of the production facilities at ORNL became underutilized. In 1989, the U.S. Department of Energy (DOE) instructed ORNL to identify and prepare various isotopes production facilities for safe shutdown. In response, ORNL identified 19 candidate facilities for shutdown and established the Isotopes Facilities Shutdown Program. In 1993, responsibility for the program was transitioned from the DOE Office of Nuclear Energy to the DOE Office of Environmental Management and Uranium Enrichment Operation's Office of Facility Transition and Management. The program was retitled the Isotopes Facilities Deactivation Project (IFDP), and implementation responsibility was transferred from ORNL to the Lockheed Martin Energy Systems, Inc. (LMES), Environmental Restoration (ER) Program

  3. Technology integration project: Environmental Restoration Technologies Department Sandia National Laboratories

    International Nuclear Information System (INIS)

    Williams, C.V.; Burford, T.D.

    1996-08-01

    Sandia National Laboratories Environmental Restoration Technologies Department is developing environmental restoration technologies through funding form the US Department of Energy's (DOE's) Office of Science and Technology. Initially, this technology development has been through the Mixed Waste Landfill Integrated Demonstration (MWLID). It is currently being developed through the Contaminant Plume containment and Remediation Focus Area, the Landfill Stabilization Focus Area, and the Characterization, Monitoring, and Sensor Cross-Cutting Program. This Technology Integration Project (TIP) was responsible for transferring MWLID-developed technologies for routine use by environmental restoration groups throughout the DOE complex and commercializing these technologies to the private sector. The MWLID's technology transfer/commercialization successes were achieved by involving private industry in development, demonstration, and technology transfer/commercialization activities; gathering and disseminating information about MWLID activities and technologies; and promoting stakeholder and regulatory involvement. From FY91 through FY95, 30 Technical Task Plans (TTPs) were funded. From these TTPs, the MWLID can claim 15 technology transfer/commercialization successes. Another seven technology transfer/commercialization successes are expected. With the changeover to the focus areas, the TIP continued the technology transfer/commercialization efforts begun under the MWLID

  4. Technology integration project: Environmental Restoration Technologies Department Sandia National Laboratories

    Energy Technology Data Exchange (ETDEWEB)

    Williams, C.V.; Burford, T.D. [Sandia National Labs., Albuquerque, NM (United States). Environmental Restoration Technologies; Allen, C.A. [Tech Reps, Inc., Albuquerque, NM (United States)

    1996-08-01

    Sandia National Laboratories Environmental Restoration Technologies Department is developing environmental restoration technologies through funding form the US Department of Energy`s (DOE`s) Office of Science and Technology. Initially, this technology development has been through the Mixed Waste Landfill Integrated Demonstration (MWLID). It is currently being developed through the Contaminant Plume containment and Remediation Focus Area, the Landfill Stabilization Focus Area, and the Characterization, Monitoring, and Sensor Cross-Cutting Program. This Technology Integration Project (TIP) was responsible for transferring MWLID-developed technologies for routine use by environmental restoration groups throughout the DOE complex and commercializing these technologies to the private sector. The MWLID`s technology transfer/commercialization successes were achieved by involving private industry in development, demonstration, and technology transfer/commercialization activities; gathering and disseminating information about MWLID activities and technologies; and promoting stakeholder and regulatory involvement. From FY91 through FY95, 30 Technical Task Plans (TTPs) were funded. From these TTPs, the MWLID can claim 15 technology transfer/commercialization successes. Another seven technology transfer/commercialization successes are expected. With the changeover to the focus areas, the TIP continued the technology transfer/commercialization efforts begun under the MWLID.

  5. Biological investigations of the Sandia National Laboratories Sol se Mete Aerial Cable Facility

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, R.M.

    1994-10-01

    This report provides results of a comprehensive biological field survey performed on the Sandia National Laboratories Aerial Cable Facility, at the east end of Kirtland Air Force Base (KAFB), Bernalillo County, New Mexico. This survey was conducted late September through October, 1991. ACF occupies a 440-acre tract of land withdrawn by the US Forest Service (USFS) for use by KAFB, and in turn placed under operational control of SNL by the Department of Energy (DOE). All land used by SNL for ACF is part of a 15,851-acre tract of land withdrawn by the US Forest Service. In addition, a number of different organizations use the 15,851-acre area. The project area used by SNL encompasses portions of approximately six sections (3,840 acres) of US Forest Service land located within the foothills of the west side of the Manzano Mountains (East Mesa). The biological study area is used by the KAFB, the US Department of Interior, and SNL. This area includes: (1) Sol se Mete Springs and Canyon, (2) East Anchor Access Road, (3) East Anchor Site, (4) Rocket Sled Track, (5) North Arena, (6) East Instrumentation Site and Access Road, (7) West Anchor Access Road, (8) West Anchor Site, (9) South Arena, (10) Winch Sites, (11) West Instrumentation Sites, (12) Explosive Assembly Building, (13) Control Building, (14) Lurance Canyon Road and vicinity. Although portions of approximately 960 acres of withdrawn US Forest Service land have been altered, only 700 acres have been disturbed by activities associated with ACF; approximately 2,880 acres consist of natural habitat. Absence of grazing by livestock and possibly native ungulates, and relative lack of human disturbance have allowed this area to remain in a more natural vegetative state relative to the condition of private range lands throughout New Mexico. This report evaluates threatened and endangered species found on ACF, as well as a comprehensive assessment of biological habitats.

  6. Needs analysis and project schedule for the Los Alamos National Laboratory (LANL) Health Physics Analysis Laboratory (HPAL) upgrade

    International Nuclear Information System (INIS)

    Rhea, T.A.; Rucker, T.L.; Stafford, M.W.

    1990-01-01

    This report is a needs assessment and project schedule for the Health Physics Analysis Laboratory (HPAL) upgrade project at Los Alamos National Laboratory (LANL). After reviewing current and projected HPAL operations, two custom-developed laboratory information management systems (LIMS) for similar facilities were reviewed; four commercially available LIMS products were also evaluated. This project is motivated by new regulations for radiation protection and training and by increased emphasis on quality assurance (QA). HPAL data are used to: protect the health of radiation workers; document contamination levels for transportation of radioactive materials and for release of materials to the public for uncontrolled use; and verify compliance with environmental emission regulations. Phase 1 of the HPAL upgrade project concentrates on four types of counting instruments which support in excess of 90% of the sample workload at the existing central laboratories. Phase 2 is a refinement phase and also integrates summary-level databases on the central Health, Safety, and Environment (HSE) VAX. Phase 3 incorporates additional instrument types and integrates satellite laboratories into the HPAL LIMS. Phase 1 will be a multi-year, multimillion dollar project. The temptation to approach the upgrade of the HPAL program in a piece meal fashion should be avoided. This is a major project, with clearly-defined goals and priorities, and should be approached as such. Major programmatic and operational impacts will be felt throughout HSE as a result of this upgrade, so effective coordination with key customer contacts will be critical

  7. The status of electronic laboratory notebooks for chemistry and biology.

    Science.gov (United States)

    Taylor, Keith T

    2006-05-01

    Documenting an experiment in a way that ensures that the record can act as evidence to support a patent claim or to demonstrate compliance with the US Food and Drug Administration's (FDA's) predicate rules, puts demands on an electronic laboratory notebook (ELN) that are not trivial. The 1996 General Agreement on Tariffs and Trade (GATT) allowed notebook records that were generated outside of the US to be used to claim precedence in US patent claims. This agreement spurred interest in the development of ELNs in Europe. The pharmaceutical research process became dependent on computer systems during the latter part of the 1990s, and this also led to a wider interest in ELNs. More recently, the FDA began to encourage submissions in an all-electronic form, leading to great interest in the use of ELNs in development and manufacturing. As a result of these influences, the pharmaceutical industry is now actively pursuing ELN evaluations and implementations. This article describes some of the early efforts and the recent drivers for ELN adoption. The state of the ELN market in 2005 is also described.

  8. Plant Biology and Biogeochemistry Department annual project report 1999

    DEFF Research Database (Denmark)

    Jensen, A.; Gissel Nielsen, G.; Giese, H.

    2000-01-01

    The Department of Plant Biology and Biogeochemistry is engaged in basic and applied research to improve the scientific knowledge of developing new methods and technology for the future, environmentally benign industrial and agricultural production, thusexerting less stress and strain...... of Biomass, 3. DLF-Risø Biotechnology, 4. Plant Genetics and Epidemiology, 5. Biogeochemistry and 6. Plant Ecosystems and Nutrient Cycling. This electronicversion of the annual report from the Plant Biology and Biogeochemistry Department aims to provide information about the progress in our research. Each...... on the environment. This knowledge will lead to a greater prosperity and welfare for agriculture, industry and consumers in Denmark. The research approach in the Department is mainly experimental and the projects areorganized in six research programmes: 1. Plant-Microbe Symbioses, 2. Plant Products and Recycling...

  9. Building Design Guidelines of Interior Architecture for Bio safety Levels of Biology Laboratories

    International Nuclear Information System (INIS)

    ElDib, A.A.

    2014-01-01

    This paper discusses the pivotal role of the Interior Architecture As one of the scientific disciplines minute to complete the Architectural Sciences, which relied upon the achievement and development of facilities containing scientific research laboratories, in terms of planning and design, particularly those containing biological laboratories using radioactive materials, adding to that, the application of the materials or raw materials commensurate with each discipline of laboratory and its work nature, and by the discussion the processing of design techniques and requirements of interior architecture dealing with Research Laboratory for electronic circuits an their applications with the making of its prototypes

  10. 76 FR 56406 - Science and Technology Reinvention Laboratory Demonstration Project; Department of the Army; Army...

    Science.gov (United States)

    2011-09-13

    ... DEPARTMENT OF DEFENSE Office of the Secretary Science and Technology Reinvention Laboratory Demonstration Project; Department of the Army; Army Research, Development and Engineering Command; Tank... personnel management demonstration project for eligible TARDEC employees. Within that notice the table...

  11. Exploring protein structure and dynamics through a project-oriented biochemistry laboratory module.

    Science.gov (United States)

    Lipchock, James M; Ginther, Patrick S; Douglas, Bonnie B; Bird, Kelly E; Patrick Loria, J

    2017-09-01

    Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant human enzyme, protein tyrosine phosphatase 1B (PTP1B). Over the course of the semester students guide their own mutant of PTP1B from conception to characterization in a cost-effective manner and gain exposure to fundamental techniques in biochemistry, including site-directed DNA mutagenesis, bacterial recombinant protein expression, affinity column purification, protein quantitation, SDS-PAGE, and enzyme kinetics. This project-based approach allows an instructor to simulate a research setting and prepare students for productive research beyond the classroom. Potential modifications to expand or contract this module are also provided. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(5):403-410, 2017. © 2017 The International Union of Biochemistry and Molecular Biology.

  12. Environmental Assessment for moving the Pacific Northwest Laboratory radon generators from Life Sciences Laboratory II, Richland North Area, to Life Sciences Laboratory I, 300 Area, and their continued use in physical and biological research

    International Nuclear Information System (INIS)

    Nelson, I.C.

    1993-01-01

    The Pacific Northwest Laboratory (PNL) radon generators are a core resource of the overall U. S. Department of Energy's (DOE) Radon Research Program and are administratively controlled within the ''Radon Hazards in Homes'' project. This project primarily focuses on radon exposures of animals and addresses the major biologic effects and factors influencing risks of indoor radon exposures. For example, the ''Mechanisms of Radon Injury'' and ''In vivo/In vitro Radon-Induced Cellular Damage'' projects specifically address the cytogenetic and DNA damage produced by radon exposure as part of a larger effort to understand radon carcinogenesis. Several other ongoing PNL projects, namely: ''Biological Effectiveness of Radon Alpha Particles: A Microbeam Study of Dose Rate Effects,'' ''Laser Measurements of Pb-210,'' ''Radon Transport Modeling in Soils,'' ''Oncogenes in Radiation Carcinogenesis,'' ''Mutation of DNA Targets,'' ''Dosimetry of Radon Progeny,'' and ''Aerosol Technology Development'' also use the radon exposure facilities in the conduct of their work. While most, but not all, studies in the PNL Radon Research Program are funded through DOE's Office of Health and Environmental Research, PNL also has ongoing collaborative radon studies with investigators worldwide; many of these use the radon exposure facilities. The purpose of the proposed action is to provide for relocation of the radon generators to a DOE-owned facility and to continue to provide a controlled source of radon-222 for continued use in physical and biological research

  13. Data management and data enrichment for systems biology projects.

    Science.gov (United States)

    Wittig, Ulrike; Rey, Maja; Weidemann, Andreas; Müller, Wolfgang

    2017-11-10

    Collecting, curating, interlinking, and sharing high quality data are central to de.NBI-SysBio, the systems biology data management service center within the de.NBI network (German Network for Bioinformatics Infrastructure). The work of the center is guided by the FAIR principles for scientific data management and stewardship. FAIR stands for the four foundational principles Findability, Accessibility, Interoperability, and Reusability which were established to enhance the ability of machines to automatically find, access, exchange and use data. Within this overview paper we describe three tools (SABIO-RK, Excemplify, SEEK) that exemplify the contribution of de.NBI-SysBio services to FAIR data, models, and experimental methods storage and exchange. The interconnectivity of the tools and the data workflow within systems biology projects will be explained. For many years we are the German partner in the FAIRDOM initiative (http://fair-dom.org) to establish a European data and model management service facility for systems biology. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  14. Biologically Weighted Quantities in Radiotherapy: an EMRP Joint Research Project

    Directory of Open Access Journals (Sweden)

    Rabus Hans

    2014-01-01

    Full Text Available Funded within the European Metrology Research Programme (EMRP [1], the joint research projectBiologically weighted quantities in radiotherapy” (BioQuaRT [2] aims to develop measurement and simulation techniques for determining the physical properties of ionising particle tracks on different length scales (about 2 nm to 10 μm, and to investigate the correlation of these track structure characteristics with the biological effects of radiation at the cellular level. Work package 1 develops micro-calorimeter prototypes for the direct measurement of lineal energy and will characterise their response for different ion beams by experiment and modelling. Work package 2 develops techniques to measure particle track structure on different length scales in the nanometre range as well as a measurement device integrating a silicon microdosimeter and a nanodosimeter. Work package 3 investigates the indirect effects of radiation based on probes for quantifying particular radical and reactive oxygen species (ROS. Work package 4 focuses on the biological aspects of radiation damage and will produce data on initial DNA damage and late effects for radiotherapy beams of different qualities. Work package 5 provides evaluated data sets of DNA cross-sections and develops a multi-scale model to address microscopic and nanometric track structure properties. The project consortium includes three linked researchers holding so-called Researcher Excellence Grants, who carry out ancillary investigations such as developing and benchmarking a new biophysical model for induction of early radiation damage and developing methods for the translation of quantities derived from particle track structure to clinical applications in ion beam therapy.

  15. Virtual Lab Demonstrations Improve Students’ Mastery of Basic Biology Laboratory Techniques

    OpenAIRE

    Maldarelli, Grace A.; Hartmann, Erica M.; Cummings, Patrick J.; Horner, Robert D.; Obom, Kristina. M.; Shingles, Richard; Pearlman, Rebecca S.

    2009-01-01

    Biology laboratory classes are designed to teach concepts and techniques through experiential learning. Students who have never performed a technique must be guided through the process, which is often difficult to standardize across multiple lab sections. Visual demonstration of laboratory procedures is a key element in teaching pedagogy. The main goals of the study were to create videos explaining and demonstrating a variety of lab techniques that would serve as teaching tools for undergradu...

  16. Human genome project: revolutionizing biology through leveraging technology

    Science.gov (United States)

    Dahl, Carol A.; Strausberg, Robert L.

    1996-04-01

    The Human Genome Project (HGP) is an international project to develop genetic, physical, and sequence-based maps of the human genome. Since the inception of the HGP it has been clear that substantially improved technology would be required to meet the scientific goals, particularly in order to acquire the complete sequence of the human genome, and that these technologies coupled with the information forthcoming from the project would have a dramatic effect on the way biomedical research is performed in the future. In this paper, we discuss the state-of-the-art for genomic DNA sequencing, technological challenges that remain, and the potential technological paths that could yield substantially improved genomic sequencing technology. The impact of the technology developed from the HGP is broad-reaching and a discussion of other research and medical applications that are leveraging HGP-derived DNA analysis technologies is included. The multidisciplinary approach to the development of new technologies that has been successful for the HGP provides a paradigm for facilitating new genomic approaches toward understanding the biological role of functional elements and systems within the cell, including those encoded within genomic DNA and their molecular products.

  17. A Semester-Long Project-Oriented Biochemistry Laboratory Based on "Helicobacter pylori" Urease

    Science.gov (United States)

    Farnham, Kate R.; Dube, Danielle H.

    2015-01-01

    Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically…

  18. Students' Perceptions of a Project-Based Organic Chemistry Laboratory Environment: A Phenomenographic Approach

    Science.gov (United States)

    Burrows, Nikita L.; Nowak, Montana K.; Mooring, Suazette R.

    2017-01-01

    Students can perceive the laboratory environment in a variety of ways that can affect what they take away from the laboratory course. This qualitative study characterizes undergraduate students' perspectives of a project-based Organic Chemistry laboratory using the theoretical framework of phenomenography. Eighteen participants were interviewed in…

  19. Fresh biological reference materials. Use in inter laboratory studies and as CRMs

    International Nuclear Information System (INIS)

    De Boer, J.

    1999-01-01

    Biological reference materials were prepared and packed in tins and glass jars to be used in inter laboratory studies on chlorobiphenyls and organochlorine pesticides, and trace metals, respectively. The materials were homogenised, sterilised and packed as wet tissue, which is unique for the purpose of inter laboratory studies and offers the advantage of studying the extraction and destruction steps of the analytical methods. In addition to their use in inter laboratory studies, some materials have been prepared or are being prepared as certified reference material for chlorobiphenyl analysis. (author)

  20. The Next Generation of Synthetic Biology Chassis: Moving Synthetic Biology from the Laboratory to the Field

    Science.gov (United States)

    2016-09-26

    online 2016 Sep 26. doi: 10.1021/acssynbio.6b00256. 14. ABSTRACT Escherichia coli (E. coli) has played a pivotal role in the development of genetics...Adams* U.S. Army Research Laboratory, Adelphi, Maryland 20783, United States ABSTRACT: Escherichia coli (E. coli) has played a pivotal role in the...the development of new SB chassis (Figure 1). First, a large knowledge base of the chassis needs to be established to facilitate development of accurate

  1. A comparison of student reactions to biology instruction by interactive videodisc or conventional laboratory

    Science.gov (United States)

    Leonard, William H.

    This study was designed to learn if students perceived an interactive computer/videodisc learning system to represent a viable alternative to (or extension of) the conventional laboratory for learning biology skills and concepts normally taught under classroom laboratory conditions. Data were collected by questionnaire for introductory biology classes at a large midwestern university where students were randomly assigned to two interactive videodisc/computer lessons titled Respiration and Climate and Life or traditional laboratory investigation with the same titles and concepts. The interactive videodisc system consisted of a TRS-80 Model III microcomputer interfaced to a Pioneer laser-disc player and a color TV monitor. Students indicated an overall level satisfaction with this strategy very similar to that of conventional laboratory instruction. Students frequently remarked that videodisc instruction gave them more experimental and procedural options and more efficient use of instructional time than did the conventional laboratory mode. These two results are consistent with past CAI research. Students also had a strong perception that the images on the videodisc were not real and this factor was perceived as having both advantages and disadvantages. Students found the two approaches to be equivalent to conventional laboratory instruction in the areas of general interest, understanding of basic principles, help on examinations, and attitude toward science. The student-opinion data in this study do not suggest that interactive videodisc technology serve as a substitute to the wet laboratory experience, but that this medium may enrich the spectrum of educational experiences usually not possible in typical classroom settings.

  2. Cloning Yeast Actin cDNA Leads to an Investigative Approach for the Molecular Biology Laboratory

    Science.gov (United States)

    Black, Michael W.; Tuan, Alice; Jonasson, Erin

    2008-01-01

    The emergence of molecular tools in multiple disciplines has elevated the importance of undergraduate laboratory courses that train students in molecular biology techniques. Although it would also be desirable to provide students with opportunities to apply these techniques in an investigative manner, this is generally not possible in the…

  3. A Statistical Analysis of Student Questions in a Cell Biology Laboratory

    Science.gov (United States)

    Keeling, Elena L.; Polacek, Kelly M.; Ingram, Ella L.

    2009-01-01

    Asking questions is an essential component of the practice of science, but question-asking skills are often underemphasized in science education. In this study, we examined questions written by students as they prepared for laboratory exercises in a senior-level cell biology class. Our goals were to discover 1) what types of questions students…

  4. An Open-Ended Investigative Microbial Ecology Laboratory for Introductory Biology

    Science.gov (United States)

    Jones-Held, Susan; Paoletti, Robert; Glick, David; Held, Michael E.

    2010-01-01

    In this article we describe a multi-week investigative laboratory in microbial ecology/diversity and nitrogen cycling that we have used in our introductory biology course. This module encourages active student involvement in experimental design, using the scientific literature and quantitative analysis of large data sets. Students analyze soil…

  5. Inquiry-based Investigation in Biology Laboratories: Does Neem Provide Bioprotection against Bean Beetles?

    Science.gov (United States)

    Pearce, Amy R.; Sale, Amanda Lovelace; Srivatsan, Malathi; Beck, Christopher W.; Blumer, Lawrence S.; Grippo, Anne A.

    2013-01-01

    We developed an inquiry-based biology laboratory exercise in which undergraduate students designed experiments addressing whether material from the neem tree ("Azadirachta indica") altered bean beetle ("Callosobruchus maculatus") movements and oviposition. Students were introduced to the bean beetle life cycle, experimental…

  6. Variability of Biological Degradation of Aromatic Hydrocarbons in an Aerobic Aquifer Determined by Laboratory Batch Experiments

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Christensen, Thomas Højlund

    1994-01-01

    The biological aerobic degradation of 7 aromatic hydrocarbons (benzene, toluene, o-xylene, p-dichlorobenzene, o-dichlorobenzene, naphthalene and biphenyl) was studied for 149 days in replicate laboratory batch experiments with groundwater and sediment from 8 localities representing a 15 m × 30 m...

  7. Glucose Transport in Cultured Animal Cells: An Exercise for the Undergraduate Cell Biology Laboratory

    Science.gov (United States)

    Ledbetter, Mary Lee S.; Lippert, Malcolm J.

    2002-01-01

    Membrane transport is a fundamental concept that undergraduate students of cell biology understand better with laboratory experience. Formal teaching exercises commonly used to illustrate this concept are unbiological, qualitative, or intricate and time consuming to prepare. We have developed an exercise that uses uptake of radiolabeled nutrient…

  8. Audio-Tutorial Versus Conventional Lecture-Laboratory Instruction in a University Animal Biology Course.

    Science.gov (United States)

    Rowsey, Robert E.

    The purpose of this study was to analyze two methods of instruction used in an animal biology course. One group of students, the experimental group, was taught using an audio-tutorial program, and another group, the control group, was taught using the conventional lecture-laboratory method. Pretest and posttest data were collected from achievement…

  9. Quality Indicators in Laboratory Medicine: the status of the progress of IFCC Working Group "Laboratory Errors and Patient Safety" project.

    Science.gov (United States)

    Sciacovelli, Laura; Lippi, Giuseppe; Sumarac, Zorica; West, Jamie; Garcia Del Pino Castro, Isabel; Furtado Vieira, Keila; Ivanov, Agnes; Plebani, Mario

    2017-03-01

    The knowledge of error rates is essential in all clinical laboratories as it enables them to accurately identify their risk level, and compare it with those of other laboratories in order to evaluate their performance in relation to the State-of-the-Art (i.e. benchmarking) and define priorities for improvement actions. Although no activity is risk free, it is widely accepted that the risk of error is minimized by the use of Quality Indicators (QIs) managed as a part of laboratory improvement strategy and proven to be suitable monitoring and improvement tools. The purpose of QIs is to keep the error risk at a level that minimizes the likelihood of patients. However, identifying a suitable State-of-the-Art is challenging, because it calls for the knowledge of error rates measured in a variety of laboratories throughout world that differ in their organization and management, context, and the population they serve. Moreover, it also depends on the choice of the events to keep under control and the individual procedure for measurement. Although many laboratory professionals believe that the systemic use of QIs in Laboratory Medicine may be effective in decreasing errors occurring throughout the total testing process (TTP), to improve patient safety as well as to satisfy the requirements of International Standard ISO 15189, they find it difficult to maintain standardized and systematic data collection, and to promote continued high level of interest, commitment and dedication in the entire staff. Although many laboratories worldwide express a willingness to participate to the Model of QIs (MQI) project of IFCC Working Group "Laboratory Errors and Patient Safety", few systematically enter/record their own results and/or use a number of QIs designed to cover all phases of the TTP. Many laboratories justify their inadequate participation in data collection of QIs by claiming that the number of QIs included in the MQI is excessive. However, an analysis of results suggests

  10. AI/Simulation Fusion Project at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Erickson, S.A.

    1984-04-25

    This presentation first discusses the motivation for the AI Simulation Fusion project. After discussing very briefly what expert systems are in general, what object oriented languages are in general, and some observed features of typical combat simulations, it discusses why putting together artificial intelligence and combat simulation makes sense. We then talk about the first demonstration goal for this fusion project.

  11. AI/Simulation Fusion Project at Lawrence Livermore National Laboratory

    International Nuclear Information System (INIS)

    Erickson, S.A.

    1984-01-01

    This presentation first discusses the motivation for the AI Simulation Fusion project. After discussing very briefly what expert systems are in general, what object oriented languages are in general, and some observed features of typical combat simulations, it discusses why putting together artificial intelligence and combat simulation makes sense. We then talk about the first demonstration goal for this fusion project

  12. IYPT problems as an efficient source of ideas for first-year project laboratory tasks

    Energy Technology Data Exchange (ETDEWEB)

    Planinsic, Gorazd [Faculty for Mathematics and Physics, University of Ljubljana (Slovenia)], E-mail: gorazd.planinsic@fmf.uni-lj.si

    2009-11-15

    In the project laboratory, a group of students are given a well-defined task but the path to the solution is entirely left to the students. The paper reports on some basic strategies in designing successful project tasks that are based on modified problems from International Young Physicists Tournament (IYPT). In addition, an integration of first-year project laboratory in in-service teacher training is also briefly presented.

  13. IYPT problems as an efficient source of ideas for first-year project laboratory tasks

    International Nuclear Information System (INIS)

    Planinsic, Gorazd

    2009-01-01

    In the project laboratory, a group of students are given a well-defined task but the path to the solution is entirely left to the students. The paper reports on some basic strategies in designing successful project tasks that are based on modified problems from International Young Physicists Tournament (IYPT). In addition, an integration of first-year project laboratory in in-service teacher training is also briefly presented.

  14. Radiation protection - Performance criteria for service laboratories performing biological dosimetry by cytogenetics

    International Nuclear Information System (INIS)

    2004-01-01

    This International Standard provides criteria for quality assurance and quality control, evaluation of the performance and the accreditation of biological dosimetry by cytogenetic service laboratories. This International Standard addresses: a) the confidentiality of personal information, for the customer and the service laboratory, b) the laboratory safety requirements, c) the calibration sources and calibration dose ranges useful for establishing the reference dose-effect curves allowing the dose estimation from chromosome aberration frequency, and the minimum detection levels, d) the scoring procedure for unstable chromosome aberrations used for biological dosimetry, e) the criteria for converting a measured aberration frequency into an estimate of absorbed dose, f) the reporting of results, g) the quality assurance and quality control, h) informative annexes containing examples of a questionnaire, instructions for customers, a data sheet for recording aberrations and a sample report

  15. Laboratory robotics projects in the Analytical Development Division at the Savannah River Laboratory

    International Nuclear Information System (INIS)

    Lien, O.G.; Steele, A.W.

    1986-01-01

    To encourage the application of robotics technology for routine radiobench applications, a laboratory dedicated to the research and development of contained robotic systems is being constructed. The facility will have several robots located in laminar flow hoods, and the hoods are being designed to allow the possibility for multiple robots to work together. This paper presents both the design features of the hoods and the general layout of the laboratory, and also discusses an application of a robotic system for the routine nuclear counting of gamma tube samples. The gamma tube system is presently operating in one of the routine analysis laboratories. 5 figs

  16. DOE outfits two laboratories for particle-physics project

    CERN Multimedia

    Hampton, T

    2003-01-01

    Scheduled for operation in early 2005, a new facility will help researchers study elusive subatomic neutrinos by projecting a particle stream from Illinois to Minnesota - through 460 miles of solid earth (1 page).

  17. Using Independent Research Projects to Foster Learning in the Comparative Vertebrate Anatomy Laboratory

    Science.gov (United States)

    Ghedotti, Michael J.; Fielitz, Christopher; Leonard, Daniel J.

    2005-01-01

    This paper presents a teaching methodology involving an independent research project component for use in undergraduate Comparative Vertebrate Anatomy laboratory courses. The proposed project introduces cooperative, active learning in a research context to comparative vertebrate anatomy. This project involves pairs or groups of three students…

  18. Compendium of the Environmental Measurements Laboratory's research projects related to the Chernobyl nuclear accident

    Energy Technology Data Exchange (ETDEWEB)

    Volchok, H L; Chieco, N [comps.

    1986-10-01

    Following the accident at the Chernobyl nuclear reactor power station in the USSR on April 26, 1986, the Environmental Measurements Laboratory (EML) initiated a number of research projects as follows: (1) selected sites in both the Deposition and Surface Air networks were alerted and their sampling protocols adjusted to accommodate the anticipated arrival times and activity concentrations of the Chernobyl debris; (2) a number of cooperative programs involving field work, sampling, analysis and data interpretation were set up with institutions and scientists in other countries; (3) EML's Regional Baseline Station at Chester, NJ, as well as the roof of the Laboratory in New York City, provided bases for sampling and measurements to study the radionuclide concentrations, radiation levels, physical characteristics and potential biological implications of the Chernobyl fallout on the northeastern United States; and (4) the resulting fallout from the Chernobyl accident provided an 'experiment of opportunity' in that it enabled us to study fresh fission product deposition using collection systems resurrected from the 1950's and 1960's for comparison with current state-of-the-art methodology. The 13 reports of this volume have been entered separately into the data base.

  19. Overview of criminal justice projects at Sandia National Laboratories

    Energy Technology Data Exchange (ETDEWEB)

    Spencer, D.D.

    1995-07-01

    The criminal justice projects at SNL include three projects for the National Institute of Justice (smart gun, restraining foam, aqueous foam, corrections perimeter), a Southwest Border study, and one involving corrections agencies. It is concluded that the national technologies developed to protect nuclear and other high value assets have enormous potential for application to crime and personal safety; the difficulty lies in simplifying the technology transfer and making the new systems affordable.

  20. Synthesized research report in the second mid-term research phase. Mizunami Underground Research Laboratory project, Horonobe Underground Research Laboratory project and geo-stability project (Translated document)

    International Nuclear Information System (INIS)

    Hama, Katsuhiro; Sasao, Eiji; Iwatsuki, Teruki; Onoe, Hironori; Sato, Toshinori; Yasue, Kenichi; Asamori, Koichi; Niwa, Masakazu; Osawa, Hideaki; Nagae, Isako; Natsuyama, Ryoko; Fujita, Tomoo; Sasamoto, Hiroshi; Matsuoka, Toshiyuki; Takeda, Masaki; Aoyagi, Kazuhei; Nakayama, Masashi; Miyakawa, Kazuya; Ito, Hiroaki; Ohyama, Takuya; Senba, Takeshi; Amano, Kenji

    2016-08-01

    We have synthesized the research results from the Mizunami/Horonobe Underground Research Laboratories (URLs) and geo-stability projects in the second mid-term research phase. This report can be used as a technical basis for the Nuclear Waste Management Organization of Japan/Regulator at each decision point from siting to beginning of disposal (Principal Investigation to Detailed Investigation Phase). High-quality construction techniques and field investigation methods have been developed and implemented, which will be directly applicable to the National Disposal Program (together with general assessments of hazardous natural events and processes). Acquisition of technical knowledge on decisions of partial backfilling and final closure from actual field experiments in the Mizunami/Horonobe URLs will be crucial as the main theme for the next phases. (author)

  1. Integration of a zebrafish research project into a molecular biology course to support critical thinking and course content goals.

    Science.gov (United States)

    Felzien, Lisa K

    2016-11-12

    Engaging undergraduates in research is essential for teaching them to think like scientists, and it has become a desired component of classroom and laboratory instruction. Research projects that span an entire semester expose students to a variety of concepts and techniques and allow students to use experiments to learn scientific principles, understand why specific techniques are applicable, critically analyze varied data, and examine how experimentation leads to acquiring knowledge. To provide an experience with these features, a semester long research project was integrated into a combined lecture and laboratory course, Molecular Biology. The project utilized the zebrafish model to examine gene expression during embryonic development and required students to develop and test hypotheses about the timing of expression of previously uncharacterized genes. The main goals for the project were to provide opportunities for students to develop critical thinking skills required for conducting research and to support the content goals of the course. To determine whether these goals were met, student performance on the steps of the project and related pre-test and post-test questions was examined. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(6):565-573, 2016. © 2016 The International Union of Biochemistry and Molecular Biology.

  2. Biological Risks and Laboratory-Acquired Infections: A Reality That Cannot be Ignored in Health Biotechnology

    Science.gov (United States)

    Coelho, Ana Cláudia; García Díez, Juan

    2015-01-01

    Advances and research in biotechnology have applications over a wide range of areas, such as microbiology, medicine, the food industry, agriculture, genetically modified organisms, and nanotechnology, among others. However, research with pathogenic agents, such as virus, parasites, fungi, rickettsia, bacterial microorganisms, or genetic modified organisms, has generated concern because of their potential biological risk – not only for people, but also for the environment due to their unpredictable behavior. In addition, concern for biosafety is associated with the emergence of new diseases or re-emergence of diseases that were already under control. Biotechnology laboratories require biosafety measures designed to protect their staff, the population, and the environment, which may be exposed to hazardous organisms and materials. Laboratory staff training and education is essential, not only to acquire a good understanding about the direct handling of hazardous biological agents but also knowledge of the epidemiology, pathogenicity, and human susceptibility to the biological materials used in research. Biological risk can be reduced and controlled by the correct application of internationally recognized procedures such as proper microbiological techniques, proper containment apparatus, adequate facilities, protective barriers, and special training and education of laboratory workers. To avoid occupational infections, knowledge about standardized microbiological procedures and techniques and the use of containment devices, facilities, and protective barriers is necessary. Training and education about the epidemiology, pathogenicity, and biohazards of the microorganisms involved may prevent or decrease the risk. In this way, the scientific community may benefit from the lessons learned in the past to anticipate future problems. PMID:25973418

  3. Biological risks and laboratory-acquired infections. A reality that cannot be ignored in health biotechnology

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Coelho

    2015-04-01

    Full Text Available Advances and research in biotechnology have applications over a wide range of areas such as microbiology, medicine, the food industry, agriculture, genetically modified organisms and nanotechnology, among others. However, research with pathogenic agents such as virus, parasites, fungi, rickettsia, bacterial microorganisms or genetic modified organisms has generated concern because of their potential biological risk - not only for people, but also for the environment due to their unpredictable behavior. In addition, concern for biosafety is associated with the emergence of new diseases or re-emergence of diseases that were already under control. Biotechnology laboratories require biosafety measures designed to protect their staff, the population and the environment, which may be exposed to hazardous organisms and materials. Laboratory staff training and education is essential, not only to acquire a good understanding about the direct handling of hazardous biological agents but also knowledge of the epidemiology, pathogenicity and human susceptibility to the biological materials used in research. Biological risk can be reduced and controlled by the correct application of internationally recognized procedures such as proper microbiological techniques, proper containment apparatus, adequate facilities, protective barriers and special training and education of laboratory workers. To avoid occupational infections, knowledge about standardized microbiological procedures and techniques and the use of containment devices, facilities and protective barriers is necessary. Training and education about the epidemiology, pathogenicity and biohazards of the microorganisms involved may prevent or decrease the risk. In this way, the scientific community may benefit from the lessons learned in the past to anticipate future problems.

  4. The restoration project : decontamination of facilities from chemical, biological and radiological contamination after terrorist action

    Energy Technology Data Exchange (ETDEWEB)

    Fingas, M.; Volchek, K.; Thouin, G.; Harrison, S.; Kuang, W. [Environment Canada, Ottawa, ON (Canada). Emergencies Science Div; Velicogna, D.; Hornof, M.; Punt, M. [SAIC Canada, Ottawa, ON (Canada); Payette, P.; Duncan, L.; Best, M.; Krishnan; Wagener, S.; Bernard, K.; Majcher, M. [Public Health Agency of Canada, Ottawa, ON (Canada); Cousins, T.; Jones, T. [Defence Research and Development Canada, Ottawa, ON (Canada)

    2005-07-01

    Bioterrorism poses a real threat to the public health and national security, and the restoration of affected facilities after a chemical, biological or radiological attack is a major concern. This paper reviewed aspects of a project conducted to collect information, test and validate procedures for site restoration after a terrorist attack. The project began with a review of existing technology and then examined new technologies. Restoration included pickup, neutralization, decontamination, removal and final destruction and deposition of contaminants as well as cleaning and neutralization of material and contaminated waste from decontamination. The project was also intended to test existing concepts and develop new ideas. Laboratory scale experiments consisted of testing, using standard laboratory techniques. Radiation decontamination consisted of removal and concentration of the radioisotopes from removal fluid. General restoration guidelines were provided, as well as details of factors considered important in specific applications, including growth conditions and phases of microorganisms in biological decontamination, or the presence of inhibitors or scavengers in chemical decontamination. Various agents were proposed that were considered to have broad spectrum capability. Test surrogates for anthrax were discussed. The feasibility of enhanced oxidation processes was examined in relation to the destruction of organophosphorus, organochlorine and carbamate pesticides. The goal was to identify a process for the treatment of surfaces contaminated with pesticides. Tests included removal from carpet, porous ceiling tile, steel plates, and floor tiles. General radiation contamination procedures and techniques were reviewed, as well as radiological decontamination waste treatment. It was concluded that there is no single decontamination technique applicable for all contaminants, and decontamination methods depend on economic, social and health factors. The amount of

  5. CSI flight experiment projects of the Naval Research Laboratory

    Science.gov (United States)

    Fisher, Shalom

    1993-02-01

    The Naval Research Laboratory (NRL) is involved in an active program of CSI flight experiments. The first CSI flight experiment of the Naval Research Laboratory, the Low Power Atmospheric Compensation Experiment (LACE) dynamics experiment, has successfully measured vibrations of an orbiting satellite with a ground-based laser radar. The observations, made on January 7, 8 and 10, 1991, represent the first ever measurements of this type. In the tests, a narrowband heterodyne CO2 laser radar, operating at a wavelength of 10.6 microns, detected vibration induced differential-Doppler signatures of the LACE satellite. Power spectral densities of forced oscillations and modal frequencies and damping rates of free-damped vibrations were obtained and compared with finite element structural models of the LACE system. Another manifested flight experiment is the Advanced Controls Technology Experiment (ACTEX) designed to demonstrate active and passive damping with piezo-electric (PZT) sensors and actuators. This experiment was developed under the management of the Air Force Phillips Laboratory with integration of the experiment at NRL. It is to ride as a secondary, or 'piggyback,' experiment on a future Navy satellite.

  6. Project management plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory. Environmental Restoration Program

    International Nuclear Information System (INIS)

    1995-01-01

    The purpose of the Isotopes Facilities Deactivation Project (IFDP) is to place nineteen former isotopes production facilities at the Oak Ridge National Laboratory in a safe, stable, and environmentally sound condition suitable for an extended period of minimum surveillance and maintenance (S ampersand M) and as quickly and economically as possible. Implementation and completion of the deactivation project win further reduce the already small risks to the environment and to public safety and health. Furthermore, the project should result in significant S ampersand M cost savings in the future. The IFDP management plan has been prepared to document the project objectives, define organizational relationships and responsibilities, and outline the management control systems to be employed in the management of the project. The project has adopted a strategy to deactivate the simple facilities first, to reduce the scope of the project, and to gain experience before addressing more difficult facilities. A decision support system is being developed to identify those activities that best promote the project mission and result in largest cost savings. The Work Plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory (Energy Systems 1994) defines the project schedule, the cost estimate, and the technical approach for the project

  7. Research projects lead by the USDA Cranberry Entomology Laboratory (CEL)

    Science.gov (United States)

    1. Newly discovered native nematode species represent bio-insecticides (grad student project) a. Problems being addressed: Issues over flea beetle control; unresolved questions over pesticide use during bloom. b. Discoveries: at least two native nematodes species new to science have been discovered ...

  8. Background study of absorbed dose in biological experiments at the Modane Underground Laboratory

    Directory of Open Access Journals (Sweden)

    Lampe Nathanael

    2016-01-01

    Full Text Available Aiming to explore how biological systems respond to ultra-low background environ-ments, we report here our background studies for biological experiments in the Modane Under-ground Laboratory. We find that the minimum radioactive background for biology experiments is limited by the potassium content of the biological sample itself, coming from its nutritive me-dium, which we find in our experimental set-up to be 26 nGy hr-1. Compared to our reference radiation environment in Clermont-Ferrand, biological experiments can be conducted in the Modane laboratory with a radiation background 8.2 times lower than the reference above-ground level. As the radiation background may be further reduced by using different nutritive media, we also provide measurements of the potassium concentration by gamma spectroscopy of yeast extract (63.3±1.2 mg g-1 and tryptone (2.5±0.2 mg g-1 in order to guide media selection in future experiments.

  9. Laboratory Directed Research and Development Program FY2016 Annual Summary of Completed Projects

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2017-03-30

    ORNL FY 2016 Annual Summary of Laboratory Directed Research and Development Program (LDRD) Completed Projects. The Laboratory Directed Research and Development (LDRD) program at ORNL operates under the authority of DOE Order 413.2C, “Laboratory Directed Research and Development” (October 22, 2015), which establishes DOE’s requirements for the program while providing the Laboratory Director broad flexibility for program implementation. The LDRD program funds are obtained through a charge to all Laboratory programs. ORNL reports its status to DOE in March of each year.

  10. OCCUPATIONAL ACCIDENTS WITH BIOLOGICAL MATERIALS IN CLINICAL ANALYSIS LABORATORY: CAUSES AND CONSEQUENCES

    Directory of Open Access Journals (Sweden)

    T. M. Azevedo

    2014-07-01

    Full Text Available Accidents involving biological material can cause diseases to the professional healthcare and also bring psychosocial effects. The aim of this study was to characterize the accidents occurring with biological material with professional of clinical laboratories of Sinop-MT. Data were collected by a questionnaire consisting of sociodemographic and health variables. 21 (87.5% of respondents stated that they never suffered any kind of accident. One of the injured workers reported that there was involvement in your emotional life. It is observed underreporting of occupational accidents by employees affected, making it difficult to increase research on the subject and actions about the problem.

  11. [Experience of the development special medical technical laboratory for studies of effects caused by potent electromagnetic radiation in biologic objects].

    Science.gov (United States)

    Gorodetsky, B N; Kalyada, T V; Petrov, S V

    2015-01-01

    This article covers topics of creating special medical technical laboratory for medial and biologic studies concerning influence of potent high-frequency elecromagnetic radiation on various biologic objects. The authors gave example of such laboratory, described its construction features, purpose and main characteristics of the included devices.

  12. Using Osteoclast Differentiation as a Model for Gene Discovery in an Undergraduate Cell Biology Laboratory

    Science.gov (United States)

    Birnbaum, Mark J.; Picco, Jenna; Clements, Meghan; Witwicka, Hanna; Yang, Meiheng; Hoey, Margaret T.; Odgren, Paul R.

    2010-01-01

    A key goal of molecular/cell biology/biotechnology is to identify essential genes in virtually every physiological process to uncover basic mechanisms of cell function and to establish potential targets of drug therapy combating human disease. This article describes a semester-long, project-oriented molecular/cellular/biotechnology laboratory…

  13. Learning Cell Biology as a Team: A Project-Based Approach to Upper-Division Cell Biology

    Science.gov (United States)

    Wright, Robin; Boggs, James

    2002-01-01

    To help students develop successful strategies for learning how to learn and communicate complex information in cell biology, we developed a quarter-long cell biology class based on team projects. Each team researches a particular human disease and presents information about the cellular structure or process affected by the disease, the cellular…

  14. Recommendations for accreditation of laboratories in molecular biology of hematologic malignancies.

    Science.gov (United States)

    Flandrin-Gresta, Pascale; Cornillet, Pascale; Hayette, Sandrine; Gachard, Nathalie; Tondeur, Sylvie; Mauté, Carole; Cayuela, Jean-Michel

    2015-01-01

    Over recent years, the development of molecular biology techniques has improved the hematological diseases diagnostic and follow-up. Consequently, these techniques are largely used in the biological screening of these diseases; therefore the Hemato-oncology molecular diagnostics laboratories must be actively involved in the accreditation process according the ISO 15189 standard. The French group of molecular biologists (GBMHM) provides requirements for the implementation of quality assurance for the medical molecular laboratories. This guideline states the recommendations for the pre-analytical, analytical (methods validation procedures, quality controls, reagents), and post-analytical conditions. In addition, herein we state a strategy for the internal quality control management. These recommendations will be regularly updated.

  15. Implementation of quality control systems in laboratories in Paraguay by the participants of ARCAL LXXVI project

    International Nuclear Information System (INIS)

    Villanueva, Z.

    2004-12-01

    In the Project ARCAL LXXVII, was realized the National Course of Control of Quality of Analytic Laboratories, from 12 to 16 of April in the CNEA, Paraguay, as a result of the one mentioned course was elaborated this project whose purpose is to elaborate the necessary documentation to fulfill the requirements of administration in the Analytic Laboratories to be adapted to the system of quality according to the ISO 17025 [es

  16. MDI Biological Laboratory Arsenic Summit: Approaches to Limiting Human Exposure to Arsenic

    OpenAIRE

    Stanton, Bruce A.

    2015-01-01

    This report is the outcome of the meeting: “Environmental and Human Health Consequences of Arsenic”, held at the MDI Biological Laboratory in Salisbury Cove, Maine, August 13–15, 2014. Human exposure to arsenic represents a significant health problem worldwide that requires immediate attention according to the World Health Organization (WHO). One billion people are exposed to arsenic in food and more than 200 million people ingest arsenic via drinking water at concentrations greater than inte...

  17. The Alcohol Dehydrogenase Kinetics Laboratory: Enhanced Data Analysis and Student-Designed Mini-Projects

    Science.gov (United States)

    Silverstein, Todd P.

    2016-01-01

    A highly instructive, wide-ranging laboratory project in which students study the effects of various parameters on the enzymatic activity of alcohol dehydrogenase has been adapted for the upper-division biochemistry and physical biochemistry laboratory. Our two main goals were to provide enhanced data analysis, featuring nonlinear regression, and…

  18. A Project-Based Laboratory for Learning Embedded System Design with Industry Support

    Science.gov (United States)

    Lee, Chyi-Shyong; Su, Juing-Huei; Lin, Kuo-En; Chang, Jia-Hao; Lin, Gu-Hong

    2010-01-01

    A project-based laboratory for learning embedded system design with support from industry is presented in this paper. The aim of this laboratory is to motivate students to learn the building blocks of embedded systems and practical control algorithms by constructing a line-following robot using the quadratic interpolation technique to predict the…

  19. Radiation chemistry at the Metallurgical Laboratory, Manhattan Project, University of Chicago (1942-1947) and the Argonne National Laboratory, Argonne, IL (1947-1984)

    International Nuclear Information System (INIS)

    Gordon, S.

    1989-01-01

    The events in radiation chemistry which occurred in the Manhattan Project Laboratory and Argonne National Laboratory during World War II are reviewed. Research programmes from then until the present day are presented, with emphasis on pulse radiolysis studies. (UK)

  20. The radioactive ion beams facility project for the legnaro laboratories

    Science.gov (United States)

    Tecchio, Luigi B.

    1999-04-01

    In the frame work of the Italian participation to the project of a high intensity proton facility for the energy amplifier and nuclear waste transmutations, LNL is involving in the design and construction of prototypes of the injection system of the 1 GeV linac that consists of a RFQ (5 MeV, 30 mA) followed by a 100 MeV linac. This program has been already financially supported and the work is actually in progress. In this context, the LNL has been proposed a project for the construction of a second generation facility for the production of radioactive ion beams (RIBs) by using the ISOL method. The final goal consists in the production of neutron rich RIBs with masses ranging from 80 to 160 by using primary beams of protons, deuterons and light ions with energy of 100 MeV and 100 kW power. This project is proposed to be developed in about 10 years from now and intermediate milestones and experiments are foreseen and under consideration for the next INFN five year plan (1999-2003). In such period of time is proposed the construction of a proton/deuteron accelerator of 10 MeV energy and 10 mA current, consisting of a RFQ (5 MeV, 30 mA) and a linac (10 MeV, 10 mA), and of a neutron area dedicated to the RIBs production, to the BNCT applications and to the neutron physics. Some remarks on the production methods will be presented. The possibility of producing radioisotopes by means of the fission induced by neutrons will be investigated and the methods of production of neutrons will be discussed.

  1. The Los Alamos National Laboratory nuclear vision project

    International Nuclear Information System (INIS)

    Arthur, E.D.; Wagner, R.L. Jnr.

    1996-01-01

    The aims, methodology and approach of the Nuclear Vision Project are described. It is not an attempt to predict the future but aims to explore alternative futures and their implications. Some essential elements of the study are: the future of nuclear power and other civil applications; nuclear weapons and proliferation; the institutions associated with, and public opinions about, nuclear science and technology; and environment-related issues. The overall objective is to create a ''global vision'' of how to balance the benefits and dangers of nuclear technology. (6 figures; 12 references). (UK)

  2. Inquiry-based laboratory investigations and student performance on standardized tests in biological science

    Science.gov (United States)

    Patke, Usha

    Achievement data from the 3rd International Mathematics and Sciences Study and Program for International Student Assessment in science have indicated that Black students from economically disadvantaged families underachieve at alarming rates in comparison to White and economically advantaged peer groups. The study site was a predominately Black, urban school district experiencing underachievement. The purpose of this correlational study was to examine the relationship between students' use of inquiry-based laboratory investigations and their performance on the Biology End of Course Test, as well as to examine the relationship while partialling out the effects of student gender. Constructivist theory formed the theoretical foundation of the study. Students' perceived levels of experience with inquiry-based laboratory investigations were measured using the Laboratory Program Variable Inventory (LPVI) survey. LPVI scores of 256 students were correlated with test scores and were examined by student gender. The Pearson correlation coefficient revealed a small direct correlation between students' experience in inquiry-based laboratory investigation classes and standardized test scores on the Biology EOCT. A partial correlational analysis indicated that the correlation remained after controlling for gender. This study may prompt a change from teacher-centered to student-centered pedagogy at the local site in order to increase academic achievement for all students. The results of this study may also influence administrators and policy makers to initiate local, state, or nationwide curricular development. A change in curriculum may promote social change as students become more competent, and more able, to succeed in life beyond secondary school.

  3. National inventory of selected biological monitoring programs. Summary report of current or recently completed projects, 1976

    Energy Technology Data Exchange (ETDEWEB)

    Kemp, H. T.

    1976-10-01

    The Inventory has resulted in establishment of a series of data bases containing biological monitoring information of varying types, namely, directory of investigators, record of projects received from mail questionnaire, detailed description of selected biomonitoring projects, and bibliographic citations supporting the projects received. This report contains detailed descriptions of selected biomonitoring projects organized on a state-by-state basis and with appropriate indices.

  4. Project management plan for the gunite and associated tanks treatability studies project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-12-01

    This plan for the Gunite and Associated Tanks (GAAT) Treatability Studies Project satisfies the requirements of the program management plan for the Oak Ridge National Laboratory (ORNL) Environmental Restoration (ER) Program as established in the Program Management Plan for the Martin Marietta Energy Systems, Inc., Oak Ridge National Laboratory Site Environmental Restoration Program. This plan is a subtier of several other ER documents designed to satisfy the US Department of Energy (DOE) Order 4700.1 requirement for major systems acquisitions. This project management plan identifies the major activities of the GAAT Treatability Studies Project; establishes performance criteria; discusses the roles and responsibilities of the organizations that will perform the work; and summarizes the work breakdown structure, schedule, milestones, and cost estimate for the project

  5. IOTA interferometer project - Plans, engineering, and laboratory results

    International Nuclear Information System (INIS)

    Reasenberg, R.D.

    1990-01-01

    The Infrared-Optical Telescope Array (IOTA) is being developed by a consortium comprising Harvard University, the MIT Lincoln Laboratory, the Smithsonian Astrophysical Observatory, the University of Massachusetts at Amherst, and the University of Wyoming. The instrument is intended to generate high-resolution images of astronomical objects by bringing together beams from widely separated telescopes and combining them at a central location. The initial configuration will consist of two 0.45 m telescopes thay may range along an L-shaped track that will permit spacings in the 5 to 38 m range, at the Smithsonian's Fred L. Whipple Observatory on Mt. Hopkins. Initial tests of this configuration are expected to be conducted during the summer of 1991 and to yield both valuable engineering data and the first scientific results including diameters of stars and artificial earth satellites and a measure of the extent of some circumstellar shells. The engineering data will be applied to the refinement of IOTA, particularly to the second IOTA configuration, in which a third telescope will be added, making it possible to obtain phase closure information. 7 refs

  6. Mars Science Laboratory Flight Software Boot Robustness Testing Project Report

    Science.gov (United States)

    Roth, Brian

    2011-01-01

    On the surface of Mars, the Mars Science Laboratory will boot up its flight computers every morning, having charged the batteries through the night. This boot process is complicated, critical, and affected by numerous hardware states that can be difficult to test. The hardware test beds do not facilitate testing a long duration of back-to-back unmanned automated tests, and although the software simulation has provided the necessary functionality and fidelity for this boot testing, there has not been support for the full flexibility necessary for this task. Therefore to perform this testing a framework has been build around the software simulation that supports running automated tests loading a variety of starting configurations for software and hardware states. This implementation has been tested against the nominal cases to validate the methodology, and support for configuring off-nominal cases is ongoing. The implication of this testing is that the introduction of input configurations that have yet proved difficult to test may reveal boot scenarios worth higher fidelity investigation, and in other cases increase confidence in the robustness of the flight software boot process.

  7. 75 FR 60091 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-09-29

    ... DEPARTMENT OF DEFENSE Office of the Secretary Science and Technology Reinvention Laboratory Personnel Management Demonstration Project, Department of the Army, Army Research, Development and... project; correction. SUMMARY: On September 9, 2010 (75 FR 55199), DoD published a notice concerning the...

  8. Use of a Laboratory Field Project in an Introductory Crop Science Course.

    Science.gov (United States)

    Lane, Robert A.

    1986-01-01

    Assesses the benefits resulting from a laboratory field project and report for agricultural students in an introductory crop science course. Student responses to evaluation statements indicated that the project helped them identify crops, understand cultural and management practices, and recognize environmental influences that affect crop…

  9. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1995 quality program status report

    Energy Technology Data Exchange (ETDEWEB)

    Bolivar, S.L.

    1996-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project`s (YMP`s) quality assurance program for January 1 to September 30, 1995. The report includes major sections on program activities and trend analysis.

  10. Implementing a Student-Designed Green Chemistry Laboratory Project in Organic Chemistry

    Science.gov (United States)

    Graham, Kate J.; Jones, T. Nicholas; Schaller, Chris P.; McIntee, Edward J.

    2014-01-01

    A multiweek organic chemistry laboratory project is described that emphasizes sustainable practices in experimental design. An emphasis on student-driven development of the project is meant to mirror the independent nature of research. Students propose environmentally friendly modifications of several reactions. With instructor feedback, students…

  11. Using Zebrafish to Implement a Course-Based Undergraduate Research Experience to Study Teratogenesis in Two Biology Laboratory Courses

    Science.gov (United States)

    Chism, Grady W.; Vaughan, Martin A.; Muralidharan, Pooja; Marrs, Jim A.

    2016-01-01

    Abstract A course-based undergraduate research experience (CURE) spanning three semesters was introduced into freshman and sophomore biology classes, with the hypothesis that participation in a CURE affects skills in research, communication, and collaboration, which may help students persist in science. Student research projects were centered on the hypothesis that nicotine and caffeine exposure during early development affects gastrulation and heart development in zebrafish. First, freshmen generated original data showing distinct effects of embryonic nicotine and caffeine exposure on zebrafish heart development and function. Next, Cell Biology laboratory students continued the CURE studies and identified novel teratogenic effects of nicotine and caffeine during gastrulation. Finally, new freshmen continued the CURE research, examining additional toxicant effects on development. Students designed new protocols, made measurements, presented results, and generated high-quality preliminary data that were studied in successive semesters. By implementing this project, the CURE extended faculty research and provided a scalable model to address national goals to involve more undergraduates in authentic scientific research. In addition, student survey results support the hypothesis that CUREs provide significant gains in student ability to (1) design experiments, (2) analyze data, and (3) make scientific presentations, translating into high student satisfaction and enhanced learning. PMID:26829498

  12. Learning how scientists work: experiential research projects to promote cell biology learning and scientific process skills.

    Science.gov (United States)

    DebBurman, Shubhik K

    2002-01-01

    Facilitating not only the mastery of sophisticated subject matter, but also the development of process skills is an ongoing challenge in teaching any introductory undergraduate course. To accomplish this goal in a sophomore-level introductory cell biology course, I require students to work in groups and complete several mock experiential research projects that imitate the professional activities of the scientific community. I designed these projects as a way to promote process skill development within content-rich pedagogy and to connect text-based and laboratory-based learning with the world of contemporary research. First, students become familiar with one primary article from a leading peer-reviewed journal, which they discuss by means of PowerPoint-based journal clubs and journalism reports highlighting public relevance. Second, relying mostly on primary articles, they investigate the molecular basis of a disease, compose reviews for an in-house journal, and present seminars in a public symposium. Last, students author primary articles detailing investigative experiments conducted in the lab. This curriculum has been successful in both quarter-based and semester-based institutions. Student attitudes toward their learning were assessed quantitatively with course surveys. Students consistently reported that these projects significantly lowered barriers to primary literature, improved research-associated skills, strengthened traditional pedagogy, and helped accomplish course objectives. Such approaches are widely suited for instructors seeking to integrate process with content in their courses.

  13. A light-water detritiation project at Chalk River Laboratories

    International Nuclear Information System (INIS)

    Boniface, H.A.; Castillo, I.; Everatt, A.E.; Ryland, D.K.

    2010-01-01

    The NRU reactor rod bays is a large, open pool of water that receives hundreds of fuel rods annually, each carrying a small amount of residual tritiated heavy water. The tritium concentration of the rod bays water has risen over the years, to a level that is of concern to the operations staff and to the environment. The proposed long-term solution is to reduce the rod bays tritium concentration by direct detritiation of the water. The Combined Electrolytic-Catalytic Exchange (CECE) process is well suited to the light-water detritiation problem. With a tritium-protium separation factor greater than five, a CECE detritiation process can easily achieve the eight orders of magnitude separation required to split a tritiated light-water feed into an essentially tritium-free effluent stream and a tritiated heavy water product suitable for recycling through a heavy water upgrader. This paper describes a CECE light-water detritiation process specifically designed to reduce the tritium concentration in the NRU rod bays to an acceptable level. The conceptual design of a 600 Mg/a detritiation process has been developed and is now at the stage of project review and the beginning of detailed design. (author)

  14. Campus Eco Tours: An Integrative & Interactive Field Project for Undergraduate Biology Students

    Science.gov (United States)

    Boes, Katie E.

    2013-01-01

    Outdoor areas within or near college campuses offer an opportunity for biology students to observe the natural world and apply concepts from class. Here, I describe an engaging and integrative project where undergraduate non-major biology students work in teams to develop and present professional "eco tours." This project takes place over multiple…

  15. Teaching Cell Biology to Dental Students with a Project-Based Learning Approach.

    Science.gov (United States)

    Costa-Silva, Daniela; Côrtes, Juliana A; Bachinski, Rober F; Spiegel, Carolina N; Alves, Gutemberg G

    2018-03-01

    Although the discipline of cell biology (CB) is part of the curricula of predoctoral dental schools, students often fail to recognize its practical relevance. The aim of this study was to assess the effectiveness of a practical-theoretical project-based course in closing the gaps among CB, scientific research, and dentistry for dental students. A project-based learning course was developed with nine sequential lessons to evaluate 108 undergraduate dental students enrolled in CB classes of a Brazilian school of dentistry during 2013-16. To highlight the relevance of in vitro studies in the preclinical evaluation of dental materials at the cellular level, the students were challenged to complete the process of drafting a protocol and performing a cytocompatibility assay for a bone substitute used in dentistry. Class activities included small group discussions, scientific database search and article presentations, protocol development, lab experimentation, and writing of a final scientific report. A control group of 31 students attended only one laboratory class on the same theme, and the final reports were compared between the two groups. The results showed that the project-based learning students had superior outcomes in acknowledging the relevance of in vitro methods during biocompatibility testing. Moreover, they produced scientifically sound reports with more content on methodological issues, the relationship with dentistry, and the scientific literature than the control group (p<0.05). The project-based learning students also recognized a higher relevance of scientific research and CB to dental practice. These results suggest that a project-based approach can help contextualize scientific research in dental curricula.

  16. Project development laboratories energy fuels and oils based on NRU “MPEI”

    Science.gov (United States)

    Burakov, I. A.; Burakov, A. Y.; Nikitina, I. S.; Khomenkov, A. M.; Paramonova, A. O.; Khtoo Naing, Aung

    2017-11-01

    In the process of improving the efficiency of power plants a hot topic is the use of high-quality fuels and lubricants. In the process of transportation, preparation for use, storage and maintenance of the properties of fuels and lubricants may deteriorate, which entails a reduction in the efficiency of power plants. One of the ways to prevent the deterioration of the properties is a timely analysis of the relevant laboratories. In this day, the existence of laboratories of energy fuels and energy laboratory oil at thermal power stations is satisfactory character. However, the training of qualified personnel to work in these laboratories is a serious problem, as the lack of opportunities in these laboratories a complete list of required tests. The solution to this problem is to explore the possibility of application of methods of analysis of the properties of fuels and lubricants in the stage of training and re-training of qualified personnel. In this regard, on the basis of MPEI developed laboratory projects of solid, liquid and gaseous fuels, power and energy oils and lubricants. Projects allow for a complete list of tests required for the timely control of properties and prevent the deterioration of these properties. Assess the financial component of the implementation of the developed projects based on the use of modern equipment used for tests. Projects allow for a complete list of tests required for the timely control of properties and prevent the deterioration of these properties.

  17. A Biology Laboratory Exercise Using Macromolecule Assays to Distinguish Four Types of Milk

    Directory of Open Access Journals (Sweden)

    Charlotte W. Pratt

    2011-03-01

    Full Text Available One of the drawbacks of cookbook-style laboratory exercises for General Biology courses is that students are not challenged to develop skills in scientific reasoning, such as formulating hypotheses and designing and carrying out experiments. Several traditional laboratory curricula include exercises involving semi-quantitative colorimetric assays to detect proteins (biuret test, reducing sugars (Benedict’s test, starch (Lugol’s test, and lipids (Sudan red test in a variety of easily prepared solutions (glucose, albumin, glycine, etc. and familiar food items (lemon juice, cornstarch, egg white, etc.. An extension of this lab exercise was developed to allow students to use their knowledge of the macromolecule assays to design an experiment to distinguish four types of “milk”: whole milk, skim milk, cream, and soy milk (rice milk or almond milk could also be included.

  18. Certification of biological reference materials: participation of the Neutron Activation Laboratory (LAN-IPEN/CNEN-SP)

    International Nuclear Information System (INIS)

    Ticianelli, Regina B.; Figueiredo, Ana Maria G.

    2007-01-01

    Analytical laboratories have as one of their important goals to demonstrate their competence allowing international acceptance and comparison of analytical data. The IPEN Neutron Activation Laboratory (LAN-IPEN) has implemented its Quality Assurance Program which comprises, among other activities, the participation in intercomparison runs. As a part of this Quality Assurance Program, LAN-IPEN has participated in interlaboratorial trials to analyze two biological candidate reference materials: INCT-CF-3 Corn Flour and INCT-SBF-4 Soya Bean Flour from the Institute of Nuclear Chemistry And Technology (Warszawa, Poland). The elements Br, Ca, Co, Cs, Fe, K, Na, Rb and Zn were analyzed in the candidate reference materials by instrumental neutron activation analysis (INAA). The performance of the laboratory was statistically evaluated in relation to the consensus values for these materials using the Z-Score test. This laboratory evaluation method has been accepted as a standard by ISO/IUPAC. In the present study, adequate Z-Score values (|Z|<2) were observed for all of the analyzed elements, confirming the accuracy of the nuclear methodology employed. The contribution of LAN-IPEN in the certification of the reference materials analyzed was very important, since the results provided were used in the statistical evaluation of the certified value. (author)

  19. Cell physiology at the Mount Desert Island Biological Laboratory: a brief look back and forward

    Science.gov (United States)

    2011-01-01

    The Mount Desert Island Biological Laboratory (MDIBL) has played important roles in the development of modern physiological concepts and tools, particularly in the fields of kidney and epithelial cell physiology. Over the last decade, MDIBL has undergone remarkable growth and evolution. This article will briefly review MDIBL's past and outline its future directions. It is hoped that this overview will renew and stimulate interest in MDIBL and, in particular, will encourage an even wider community of physiologists to participate in its ongoing growth and development. PMID:21068363

  20. Variability of Biological Degradation of Phenolic Hydrocarbons in an Aerobic Aquifer Determined by Laboratory Batch Experiments

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Christensen, Thomas Højlund

    1994-01-01

    The biological aerobic degradation of 7 phenolic hydrocarbons (phenol, o-cresol, o-nitrophenol, p-nitrophenol, 2,6-dichlorophenol, 2,4-dichlorophenol, 4,6-o-dichlorocresol) and 1 aromatic hydrocarbon (nitrobenzene) was studied for 149 days in replicate laboratory batch microcosms with sediment...... and groundwater from 8 localities representing a 15 m × 30 m section of an aerobic aquifer. Three patterns of variation were found: (1) phenol, o-cresol and in most cases p-nitrophenol showed very fast degradation with no or only short lag phases and with very little variation among localities; (2) 2...

  1. Sampling and Analysis Instruction for the Demolition of the Masonry Block for the 108-F Biological Laboratory

    International Nuclear Information System (INIS)

    Byrnes, M. E.

    1999-01-01

    This sampling and analysis instruction (SAI) has been prepared to clearly define the sampling and analysis activities to be performed in support of the demolition and disposition (or disposal) of the 108-F Biological Laboratory masonry block walls

  2. Project Work in Social Biology at GCE Advanced Level

    Science.gov (United States)

    Gadd, P.; Smith, S. Tyrell

    1977-01-01

    The system by which projects are submitted, modified, and approved is outlined and an indication is given of the standards and quantity of work expected. Criteria on which assessment is based are explained, the range of individual studies is summarized, and cases for and against project work are given. (Author/AJ)

  3. Sixth-Form Projects in Biology: A Case History.

    Science.gov (United States)

    Robinson, P. M.; Parker, R. E.

    1981-01-01

    Some of the problems encountered in devising sixth-form projects are discussed and a detailed account given of one project in which a study was made of the effect of onion bulb volatiles on the germination of lettuce seed. (Author)

  4. Management of citation verification requests for multiple projects at Sandia National Laboratories

    International Nuclear Information System (INIS)

    Crawford, C.S.

    1995-01-01

    Sandia National Laboratories' (SNL) Technical Library is now responsible for providing citation verification management support for all references cited in technical reports issued by the Nuclear Waste Management (NWM) Program. This paper dancing how this process is managed for the Yucca Mountain Site Characterization (YWP), Waste Isolation Pilot Plant (WIPP), Idaho National Engineering Laboratory (INEL), and Greater Confinement Disposal (GCD) projects. Since technical reports are the main product of these projects, emphasis is placed on meeting the constantly evolving needs of these customers in a timely and cost-effective manner

  5. Lawrence Livermore National Laboratory- Completing the Human Genome Project and Triggering Nearly $1 Trillion in U.S. Economic Activity

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, Jeffrey S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-07-28

    The success of the Human Genome project is already nearing $1 Trillion dollars of U.S. economic activity. Lawrence Livermore National Laboratory (LLNL) was a co-leader in one of the biggest biological research effort in history, sequencing the Human Genome Project. This ambitious research effort set out to sequence the approximately 3 billion nucleotides in the human genome, an effort many thought was nearly impossible. Deoxyribonucleic acid (DNA) was discovered in 1869, and by 1943 came the discovery that DNA was a molecule that encodes the genetic instructions used in the development and functioning of living organisms and many viruses. To make full use of the information, scientists needed to first sequence the billions of nucleotides to begin linking them to genetic traits and illnesses, and eventually more effective treatments. New medical discoveries and improved agriculture productivity were some of the expected benefits. While the potential benefits were vast, the timeline (over a decade) and cost ($3.8 Billion) exceeded what the private sector would normally attempt, especially when this would only be the first phase toward the path to new discoveries and market opportunities. The Department of Energy believed its best research laboratories could meet this Grand Challenge and soon convinced the National Institute of Health to formally propose the Human Genome project to the federal government. The U.S. government accepted the risk and challenge to potentially create new healthcare and food discoveries that could benefit the world and the U.S. Industry.

  6. How to Generate Understanding of the Scientific Process in Introductory Biology: A Student-Designed Laboratory Exercise on Yeast Fermentation

    Science.gov (United States)

    Collins, Linda T.; Bell, Rebekah P.

    2004-01-01

    Heavy faculty teaching loads and limited funds biology teachers designed certain objectives in order to increase the understandability of the subject matter of the laboratory exercises they write. In relation to these objectives an old "cookbook" laboratory exercise on yeast fermentation is introduced which involve students asking questions,…

  7. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1994 quality program status report

    Energy Technology Data Exchange (ETDEWEB)

    Bolivar, S.L.

    1996-03-01

    This status report is for calendar year 1994. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, a baseline is established that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify adverse trends and to evaluate improvements. This is the fourth annual status report.

  8. A summary of the environmental restoration program retrieval demonstration project at the Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    McQuary, J.

    1991-02-01

    This report provides a summary of the Environmental Restoration Program's Retrieval Demonstration Project at the Idaho National Engineering Laboratory. This project developed concepts for demonstrating facilities and equipment for the retrieval of buried transuranic mixed waste at the INEL. Included is a brief assessment of the viability, cost effectiveness, and safety of retrieval based on the developed concept. Changes made in Revision 1 reflect editorial changes only. 31 refs., 1 fig

  9. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1994 quality program status report

    International Nuclear Information System (INIS)

    Bolivar, S.L.

    1996-03-01

    This status report is for calendar year 1994. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, a baseline is established that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify adverse trends and to evaluate improvements. This is the fourth annual status report

  10. Integration of a Faculty's Ongoing Research into an Undergraduate Laboratory Teaching Class in Developmental Biology

    Science.gov (United States)

    Nam, Sang-Chul

    2018-01-01

    Traditional developmental biology laboratory classes have utilized a number of different model organisms to allow students to be exposed to diverse biological phenomena in developing organisms. This traditional approach has mainly focused on the diverse morphological and anatomical descriptions of the developing organisms. However, modern…

  11. Decontamination and dismantlement of the JANUS Reactor at Argonne National Laboratory-East. Project final report

    International Nuclear Information System (INIS)

    Fellhauer, C.R.; Clark, F.R.

    1997-10-01

    The decontamination and dismantlement of the JANUS Reactor at Argonne National Laboratory-East (ANL-E) was completed in October 1997. Descriptions and evaluations of the activities performed and analyses of the results obtained during the JANUS D and D Project are provided in this Final Report. The following information is included: objective of the JANUS D and D Project; history of the JANUS Reactor facility; description of the ANL-E site and the JANUS Reactor facility; overview of the D and D activities performed; description of the project planning and engineering; description of the D and D operations; summary of the final status of the JANUS Reactor facility based upon the final survey results; description of the health and safety aspects of the project, including personnel exposure and OSHA reporting; summary of the waste minimization techniques utilized and total waste generated by the project; and summary of the final cost and schedule for the JANUS D and D Project

  12. Virtual Lab Demonstrations Improve Students’ Mastery of Basic Biology Laboratory Techniques

    Directory of Open Access Journals (Sweden)

    Grace A. Maldarelli

    2009-12-01

    Full Text Available Biology laboratory classes are designed to teach concepts and techniques through experiential learning. Students who have never performed a technique must be guided through the process, which is often difficult to standardize across multiple lab sections. Visual demonstration of laboratory procedures is a key element in teaching pedagogy. The main goals of the study were to create videos explaining and demonstrating a variety of lab techniques that would serve as teaching tools for undergraduate and graduate lab courses and to assess the impact of these videos on student learning. Demonstrations of individual laboratory procedures were videotaped and then edited with iMovie. Narration for the videos was edited with Audacity. Undergraduate students were surveyed anonymously prior to and following screening to assess the impact of the videos on student lab performance by completion of two Participant Perception Indicator surveys. A total of 203 and 171 students completed the pre- and posttesting surveys, respectively. Statistical analyses were performed to compare student perceptions of knowledge of, confidence in, and experience with the lab techniques before and after viewing the videos. Eleven demonstrations were recorded. Chi-square analysis revealed a significant increase in the number of students reporting increased knowledge of, confidence in, and experience with the lab techniques after viewing the videos. Incorporation of instructional videos as prelaboratory exercises has the potential to standardize techniques and to promote successful experimental outcomes.

  13. A statistical analysis of student questions in a cell biology laboratory.

    Science.gov (United States)

    Keeling, Elena L; Polacek, Kelly M; Ingram, Ella L

    2009-01-01

    Asking questions is an essential component of the practice of science, but question-asking skills are often underemphasized in science education. In this study, we examined questions written by students as they prepared for laboratory exercises in a senior-level cell biology class. Our goals were to discover 1) what types of questions students asked about laboratory activities, 2) whether the types or quality of questions changed over time, and 3) whether the quality of questions or degree of improvement was related to academic performance. We found a majority of questions were about laboratory outcomes or seeking additional descriptive information about organisms or processes to be studied. Few questions earned the highest possible ranking, which required demonstration of extended thought, integration of information, and/or hypotheses and future experiments, although a majority of students asked such a question at least once. We found no correlation between types of student questions or improvement in questions and final grades. Only a small improvement in overall question quality was seen despite considerable practice at writing questions about science. Our results suggest that improving students' ability to generate higher-order questions may require specific pedagogical intervention.

  14. Biological reduction of uranium-From the laboratory to the field

    International Nuclear Information System (INIS)

    Dullies, Frank; Lutze, Werner; Gong, Weiliang; Nuttall, H. Eric

    2010-01-01

    The chemical and biological processes underlying in situ bioremediation of uranium-contaminated groundwater have been studied in the laboratory and in the field. This article focuses on the long-term stability of uraninite (UO 2 ) in the underground. A large tailings pond, 'Daenkritz 1' in Germany, was selected for this investigation. A single-pass flow-through experiment was run in a 100-liter column: bioremediation for 1 year followed by infiltration of tap water (2.5 years) saturated with oxygen, sufficient to oxidize the precipitated uraninite in two months. Instead, only 1 wt.% uraninite was released over 2.4 years at concentrations typically less than 20 μg/L. Uraninite was protected against oxidation by the mineral mackinawite (FeS 0.9 ), a considerable amount of which had formed, together with uraninite. A confined field test was conducted adjacent to the tailings pond, which after bio-stimulation showed similarly encouraging results as in the laboratory. Taking Daenkritz 1 as an example we show that in situ bioremediation can be a viable option for long-term site remediation, if the process is designed based on sufficient laboratory and field data. The boundary conditions for the site in Germany are discussed.

  15. Improvement of tuberculosis laboratory capacity on Pemba Island, Zanzibar: a health cooperation project.

    Directory of Open Access Journals (Sweden)

    Maria G Paglia

    Full Text Available Low-income countries with high Tuberculosis burden have few reference laboratories able to perform TB culture. In 2006, the Zanzibar National TB Control Programme planned to decentralize TB diagnostics. The Italian Cooperation Agency with the scientific support of the "L. Spallanzani" National Institute for Infectious Diseases sustained the project through the implementation of a TB reference laboratory in a low-income country with a high prevalence of TB. The implementation steps were: 1 TB laboratory design according to the WHO standards; 2 laboratory equipment and reagent supplies for microscopy, cultures, and identification; 3 on-the-job training of the local staff; 4 web- and telemedicine-based supervision. From April 2007 to December 2010, 921 sputum samples were received from 40 peripheral laboratories: 120 TB cases were diagnosed. Of all the smear-positive cases, 74.2% were culture-positive. During the year 2010, the smear positive to culture positive rate increased up to 100%. In March 20, 2010 the Ministry of Health and Social Welfare of Zanzibar officially recognized the Public Health Laboratory- Ivo de Carneri as the National TB Reference Laboratory for the Zanzibar Archipelago. An advanced TB laboratory can represent a low cost solution to strengthen the TB diagnosis, to provide capacity building and mid-term sustainability.

  16. Improvement of Student Critical Thinking Skills with the Natural Product Mini Project Laboratory Learning

    Directory of Open Access Journals (Sweden)

    Aliefman Hakim

    2016-12-01

    Full Text Available This research aims to investigate effect of learning using natural product mini project laboratory on students’ critical thinking skills. The research was conducted on sixth semester of 59 students of chemistry and chemistry education program from one of the state universities in West Nusa Tenggara, Indonesia in 2012/2013. This research revealed class where the student learn using natural product mini project laboratory had more critical thinking skills than those using verification laboratory. The average n-gain of critical thinking skills for experiment class was 0.58 while for the control class was 0.37. The highest n-gain in the experiment class was 0.70 for “deciding on an action (selecting criteria to judge possible solutions indicators”, while the smallest n-gain was 0.47 for “the making and judging value of judgments (balancing, weighing, and deciding indicators. We concluded that the natural product mini project laboratory was better than verification laboratory in improving the students’ critical thinking skills.

  17. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1995 quality program status report

    International Nuclear Information System (INIS)

    Bolivar, S.L.

    1996-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory Yucca Mountain Site Characterization Project's (YMP's) quality assurance program for January 1 to September 30, 1995. The report includes major sections on program activities and trend analysis

  18. Purification and Characterization of Taq Polymerase: A 9-Week Biochemistry Laboratory Project for Undergraduate Students

    Science.gov (United States)

    Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A.

    2010-01-01

    We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…

  19. Exploring Protein Structure and Dynamics through a Project-Oriented Biochemistry Laboratory Module

    Science.gov (United States)

    Lipchock, James M.; Ginther, Patrick S.; Douglas, Bonnie B.; Bird, Kelly E.; Loria, J. Patrick

    2017-01-01

    Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant…

  20. Results of single borehole hydraulic testing in the Mizunami Underground Research Laboratory project. Phase 2

    International Nuclear Information System (INIS)

    Daimaru, Shuji; Takeuchi, Ryuji; Onoe, Hironori; Saegusa, Hiromitsu

    2012-09-01

    This report summarize the results of the single borehole hydraulic tests of 79 sections conducted as part of the Construction phase (Phase 2) in the Mizunami Underground Research Laboratory (MIU) Project. The details of each test (test interval depth, geology, etc.) as well as the interpreted hydraulic parameters and analytical method used are presented in this report. (author)

  1. Vertical and Horizontal Integration of Laboratory Curricula and Course Projects across the Electronic Engineering Technology Program

    Science.gov (United States)

    Zhan, Wei; Goulart, Ana; Morgan, Joseph A.; Porter, Jay R.

    2011-01-01

    This paper discusses the details of the curricular development effort with a focus on the vertical and horizontal integration of laboratory curricula and course projects within the Electronic Engineering Technology (EET) program at Texas A&M University. Both software and hardware aspects are addressed. A common set of software tools are…

  2. Students Dig Deep in the Mystery Soil Lab: A Playful, Inquiry-Based Soil Laboratory Project

    Science.gov (United States)

    Thiet, Rachel K.

    2014-01-01

    The Mystery Soil Lab, a playful, inquiry-based laboratory project, is designed to develop students' skills of inquiry, soil analysis, and synthesis of foundational concepts in soil science and soil ecology. Student groups are given the charge to explore and identify a "Mystery Soil" collected from a unique landscape within a 10-mile…

  3. Los Alamos National Laboratory Yucca Mountain Site Characterization Project: 1991 quality program status report

    International Nuclear Information System (INIS)

    1992-07-01

    This status report summarizes the activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project's (YMP) quality assurance program for calendar year 1991. The report is divided into three Sections: Program Activities, Verification Activities, and Trend Analysis

  4. A Laboratory Experiment, Based on the Maillard Reaction, Conducted as a Project in Introductory Statistics

    Science.gov (United States)

    Kravchuk, Olena; Elliott, Antony; Bhandari, Bhesh

    2005-01-01

    A simple laboratory experiment, based on the Maillard reaction, served as a project in Introductory Statistics for undergraduates in Food Science and Technology. By using the principles of randomization and replication and reflecting on the sources of variation in the experimental data, students reinforced the statistical concepts and techniques…

  5. Pacific Northwest National Laboratory Apatite Investigation at the 100-NR-2 Quality Assurance Project Plan

    Energy Technology Data Exchange (ETDEWEB)

    Fix, N. J.

    2008-03-28

    This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the 100-NR-2 Apatite Project. The U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N would include apatite sequestration as the primary treatment, followed by a secondary treatment. The scope of this project covers the technical support needed before, during, and after treatment of the targeted subsurface environment using a new high-concentration formulation.

  6. Intelligent DC Microgrid Living Laboratories - A Chinese-Danish Cooperation Project

    DEFF Research Database (Denmark)

    Diaz, Enrique Rodriguez; Su, Xiaoling; Savaghebi, Mehdi

    2015-01-01

    This paper presents a research project focus on the development of future intelligent direct-current (DC) microgrids which is being deployed for highly efficient integration of distributed generation and modern electronic loads. The project is based on the collaboration between research institutes...... in China and Denmark, aiming to explore the different aspects of DC microgrids: design, modelling, control, coordination, communications and management. In addition, a future Living Laboratory will also be integrated into the Intelligent DC Microgrids Laboratory, which will serve as demonstration facility...... for low voltage direct-current (LVDC) distribution systems. Research is carried out in both Intelligent DC Microgrid Laboratories, focused on industry in China and residential applications in Denmark....

  7. Horonobe Underground Research Laboratory project investigation report for the 2008 fiscal year

    International Nuclear Information System (INIS)

    Nakayama, Masashi; Sano, Michiaki; Sanada, Hiroyuki; Sugita, Yutaka

    2009-11-01

    The Horonobe Underground Research Laboratory Project is planned to extend over a period 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations' 'Phase 2: Construction Phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the results of the investigations for the 2008 fiscal year (2008/2009), the 4th year of the Phase 2 investigations. The investigations, which are composed of 'Geoscientific research' and 'R and D on geological disposal technology', were carried out according to 'Horonobe Underground Research Laboratory Project Investigation Program for the 2008 Fiscal year'. The results of these investigations, along with the results which were obtained in other departments of Japan Atomic Energy Agency (JAEA), are properly offered to the implementations and the safety regulations. For the sake of this, JAEA has proceeded with the project in collaboration with experts from domestic and overseas research organisations. (author)

  8. Horonobe Underground Research Laboratory project. Investigation report for the 2010 fiscal year

    International Nuclear Information System (INIS)

    Nakayama, Masashi; Sawada, Sumiyuki; Sugita, Yutaka

    2011-09-01

    The Horonobe Underground Research Laboratory Project is planned to extend over a period 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations', 'Phase 2: Construction Phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the results of the investigations for the 2010 fiscal year (2010/2011). The investigations, which are composed of 'Geoscientific research' and 'R and D on geological disposal technology', were carried out according to 'Horonobe Underground Research Laboratory Project Investigation Program for the 2010 Fiscal year'. The results of these investigations, along with the results which were obtained in other departments of Japan Atomic Energy Agency (JAEA), are properly offered to the implementations and the safety regulations. For the sake of this, JAEA has proceeded with the project in collaboration with experts from domestic and overseas research organisations. (author)

  9. From gene to structure: Lactobacillus bulgaricus D-lactate dehydrogenase from yogurt as an integrated curriculum model for undergraduate molecular biology and biochemistry laboratory courses.

    Science.gov (United States)

    Lawton, Jeffrey A; Prescott, Noelle A; Lawton, Ping X

    2018-05-01

    We have developed an integrated, project-oriented curriculum for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the ldhA gene from the yogurt-fermenting bacterium Lactobacillus bulgaricus, which encodes the enzyme d-lactate dehydrogenase. The molecular biology module, which consists of nine experiments carried out over eleven sessions, begins with the isolation of genomic DNA from L. bulgaricus in yogurt and guides students through the process of cloning the ldhA gene into a prokaryotic expression vector, followed by mRNA isolation and characterization of recombinant gene expression levels using RT-PCR. The biochemistry module, which consists of nine experiments carried out over eight sessions, begins with overexpression of the cloned ldhA gene and guides students through the process of affinity purification, biochemical characterization of the purified LdhA protein, and analysis of enzyme kinetics using various substrates and an inhibitor, concluding with a guided inquiry investigation of structure-function relationships in the three-dimensional structure of LdhA using molecular visualization software. Students conclude by writing a paper describing their work on the project, formatted as a manuscript to be submitted for publication in a scientific journal. Overall, this curriculum, with its emphasis on experiential learning, provides hands-on training with a variety of common laboratory techniques in molecular biology and biochemistry and builds experience with the process of scientific reasoning, along with reinforcement of essential transferrable skills such as critical thinking, information literacy, and written communication, all within the framework of an extended project having the look and feel of a research experience. © 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):270-278, 2018. © 2018 The International Union of Biochemistry and Molecular Biology.

  10. Analytical quality assurance in laboratories using tracers for biological and environmental studies

    International Nuclear Information System (INIS)

    Melaj, Mariana; Martin, Olga; Lopez, Silvia; Rojas de Tramontini, Susana

    1999-01-01

    This work describe the way we are organizing a quality assurance system to apply in the analytical measurements of the relation 14 N/ 15 N in biological and soil material. The relation 14 / 15 is measured with a optic emission spectrometer (NOI6PC), which distinguish the differences in wave length of electromagnetic radiation emitted by N-28, N-29 and N-30. The major problem is the 'cross contamination' of samples with different enrichments. The elements that are been considered to reach satisfactory analytical results are: 1) A proper working area; 2) The samples must be homogeneous and the samples must represent the whole sampled system; 3) The use of reference materials. In each digestion, a known reference sample must be added; 4) Adequate equipment operation; 5) Standard operating procedures; 6) Control charts, laboratory and equipment books. All operations using the equipment is registered in a book; 7) Training of the operators. (author)

  11. An analysis of laboratory activities found in "Applications In Biology/Chemistry: A Contextual Approach to Laboratory Science"

    Science.gov (United States)

    Haskins, Sandra Sue

    The purpose of this study was to quantitatively determine whether the material found in ABC promotes scientific inquiry through the inclusion of science process skills, and to quantitatively determine the type (experimental, comparative, or descriptive) and character (wet-lab, paper and pencil, model, or computer) of laboratory activities. The research design allowed for an examination of the frequency and type of science process skills required of students in 79 laboratory activities sampled from all 12 units utilizing a modified 33-item laboratory analysis inventory (LAI) (Germane et al, 1996). Interrater reliability for the science process skills was completed on 19 of the laboratory activities with a mean score of 86.1%. Interrater reliability for the type and character of the laboratory, on the same 19 laboratory activities, was completed with mean scores of 79.0% and 96.5%, respectively. It was found that all laboratory activities provide a prelaboratory activity. In addition, the science process skill category of student performance is required most often of students with the skill of learning techniques or manipulating apparatus occurring 99% of the time. The science process skill category observed the least was student planning and design, occurring only 3% of the time. Students were rarely given the opportunity to practice science process skills such as developing and testing hypotheses through experiments they have designed. Chi-square tests, applied at the .05 level of significance, revealed that there was a significant difference in the type of laboratory activities; comparative laboratory activities appeared more often (59%). In addition the character of laboratory activities, "wet-lab" activities appeared more often (90%) than any of the others.

  12. First annual report on the Biological Monitoring and Abatement Program at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Loar, J.M.; Amano, H.; Jimenez, B.D.; Kitchings, J.T.; Meyers-Schoene, L.; Mohrbacher, D.A.; Olsen, C.R.

    1992-08-01

    As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the first of a series of annual reports presenting the results of BMAP, describes studies that were conducted from March through December 1986

  13. Experience of an inter-laboratory exercise for the determination of Carbon-14 in biological samples

    International Nuclear Information System (INIS)

    Baburajan, A.; Rajaram, S.; D'Souza, Renita Shiny; Nayak, Rasmi; Karunakara, N.; Ravi, P.M.; Tripathi, R.M.

    2018-01-01

    Carbon-14 is one of the naturally occurring cosmogenic nuclide with long half life of 5730 y and beta energy, E max : 156 keV produced continuously in the outer atmosphere. It is also produced by the anthropogenic activities like nuclear weapon test, nuclear power plant etc. contributing to the atmospheric inventory. The 14 CO 2 gets incorporated with the plant species during photosynthesis and ultimately reaches to man through food chain. It is important to accurately quantify the level of 14 C in different biological matrices for the computation of radiation dose due to ingestion. There are different methods available for the determination of 14 C in biological samples. The oxidation of the dried sample is one of the methods used for liberating the 14 CO 2 and which in turn re-absorbed using Carbo Sorb and subjected to Liquid scintillation analyses with Permaflour scintillator solution. The paper deals with the quality assurance programme initiated by ESL, Tarapur along with ESL, Kalpakkam and CARER, Mangalore University and share the experience of the inter-laboratory comparison exercise

  14. Illuminating cell signaling: Using Vibrio harveyi in an introductory biology laboratory.

    Science.gov (United States)

    Hrizo, Stacy L; Kaufmann, Nancy

    2009-05-01

    Cell signaling is an essential cellular process that is performed by all living organisms. Bacteria communicate with each other using a chemical language in a signaling pathway that allows bacteria to evaluate the size of their population, determine when they have reached a critical mass (quorum sensing), and then change their behavior in unison to carry out processes that require many cells acting together to be effective. Here, we describe a laboratory exercise in which the students observe the induction of bioluminescence or light production as an output of the quorum sensing pathway in Vibrio harveyi. Using both wildtype and mutant bacterial strains they explore the induction of community behavior via cell-cell communication by determining whether there is a correlation between the density of the bacterial population and the production of light by the bacterial culture. Using data from a cross-feeding assay the students make predictions about the identity of their strains and directly test these predictions using conditioned media from various liquid cultures. This two part exercise is designed for an introductory biology course to begin familiarizing students with collecting data, making predictions based upon the data and directly testing their hypotheses using a model organism with a cell signaling pathway that has a simple visual output: light production. Copyright © 2009 International Union of Biochemistry and Molecular Biology, Inc.

  15. Chloridrate of N-isopropyl-p-iodoamphetamine labeled with Iodine-131. Biological distribution in laboratory animals

    International Nuclear Information System (INIS)

    Colturato, Maria Tereza; Muramoto, Emiko; Carvalho, Olga Goncalves de

    2000-01-01

    The development of this work was based on a great interest from the medical class in the utilization of chloridrate of N-isopropyl-p-iodoamphetamine (IMP) labeled with 123 I, for brain perfusion evaluation. Studies were performed to optimize the labeling parameters of IMP with 131 I using nucleophilic substitution: temperature and, time reaction, ascorbic acid mass, pH and relation IMP mass/radioiodo activity, and stability of the final product. Radiochemistry purity method used showed to be efficient, quick and of easily handling for routine production. Biological distribution studies were performed in mice to determine the percent administered dose in the blood, different organs and whole body after intravenous administration of the radiopharmaceutical. The product crossed the intact blood brain barrier, allowing a follow up of further studies after the intravenous administration of the radiopharmaceutical. The principal elimination route 131 I-IMP was the urinary. Based on the results from radiochemical purity, stability and biological behavior in laboratory animals, we concluded that the studied radiopharmaceutical presents all ideal characteristics for clinical use in brain studies in nuclear medicine. (author)

  16. First annual report on the Biological Monitoring and Abatement Program at Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Loar, J. M. [ed.; Adams, S. M.; Blaylock, B. G.; Boston, H. L.; Frank, M. L.; Garten, C. T.; Houston, M. A.; Kimmel, B. L.; Ryon, M. G.; Smith, J. G.; Southworth, G. R.; Stewart, A. J.; Walton, B. T.; Berry, J. B.; Talmage, S. S. [Oak Ridge National Lab., TN (United States); Amano, H. [JAERI, Tokai Res., Establishment, Ibari-Ken (Japan); Jimenez, B. D. [School of Pharmacy, Univ. of Puerto Rico (San Juan); Kitchings, J. T. [ERCE, Denver, CO (United States); Meyers-Schoene, L. [Advanced Sciences, Inc., Fernald, OH (United States); Mohrbacher, D. A. [Univ. of Tennessee, Knoxville, TN (United States); Olsen, C. R. [USDOE Office of Energy Research, Washington, DC (United States). Office of Health and Environmental Research

    1992-08-01

    As a condition of the National Pollutant Discharge Elimination System (NPDES) permit issued to Oak Ridge National Laboratory (ORNL) on April 1, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for White Oak Creek (WOC); selected tributaries of WOC, including Fifth Creek, First Creek, Melton Branch, and Northwest Tributary; and the Clinch River. BMAP consists of seven major tasks that address both radiological and nonradiological contaminants in the aquatic and terrestrial environs on-site and the aquatic environs off-site. These tasks are (1) toxicity monitoring; (2) bioaccumulation monitoring of nonradiological contaminants in aquatic biota; (3) biological indicator studies; (4) instream ecological monitoring; (5) assessment of contaminants in the terrestrial environment; (6) radioecology of WOC and White Oak Lake (WOL); and (7) contaminant transport, distribution, and fate in the WOC embayment-Clinch River-Watts Bar Reservoir system. This document, the first of a series of annual reports presenting the results of BMAP, describes studies that were conducted from March through December 1986.

  17. Activities developed by the biological dosimetry laboratory of the Autoridad Regulatoria Nuclear - ARN of Argentina

    International Nuclear Information System (INIS)

    Radl, A.; Sapienza, C.E.; Taja, M.R.; Bubniak, R.; Deminge, M.; Di Giorgio, M.

    2013-01-01

    Biological dosimetry (DB) allows to estimate doses absorbed in individuals exposed to ionizing radiation through the quantification of stable and unstable chromosome aberrations (SCA and UCA). The frequency of these aberrations is referred to a calibration dose response curve (in vitro) to determine the doses of the individual to the whole body. The DB is a necessary support for programs of national radiation protection and response systems in nuclear or radiological emergencies in the event of accidental or incidental, single overexposure or large scale. In this context the Laboratory of Dosimetry Biological (LDB) of the Authority Regulatory Nuclear (ARN) Argentina develops and applies different dosimeters cytogenetic from four decades ago. These dosimeters provide a fact more within the whole of the information necessary for an accidental, complementing the physical and clinical dosimetry exposure assessment. The most widely used in the DB biodosimetric method is the quantification of SCA (dicentrics and rings Central) from a sample of venous blood. The LDB is accredited for the trial, under rules IRAM 301: 2005 (ISO / IEC 17025: 2005) and ISO 19238:2004. Test applies to the immediate dosimetry evaluation of acute exposures, all or a large part of the body in the range 0,1-5 Gy. In this context the LDB is part of the Latin American network of DB (LBDNet), BioDoseNet-who and response system in radiological emergencies and nuclear IAEA-RANET, being enabled to summon the LBDNet if necessary

  18. Tested Studies for Laboratory Teaching. Proceedings of the Workshop/Conference of the Association for Biology Laboratory Education (ABLE) (15th, Toronto, Ontario, Canada, June 8-12, 1993). Volume 15.

    Science.gov (United States)

    Goldman, Corey A., Ed.

    The focus of the Association for Biology Laboratory Education (ABLE) is to improve the undergraduate biology laboratory experience by promoting the development and dissemination of interesting, innovative, and reliable laboratory exercises. This proceedings volume contains 18 papers: "Human DNA Fingerprinting by Polymerase Chain Reaction" (M. V.…

  19. Tested Studies for Laboratory Teaching. Proceedings of the Workshop/Conference of the Association for Biology Laboratory Education (ABLE) (13th, Laramie, Wyoming, June 11-15, 1991). Volume 13.

    Science.gov (United States)

    Goldman, Corey A., Ed.

    The focus of the Association for Biology Laboratory Education (ABLE) is to improve the undergraduate biology laboratory experience by promoting the development and dissemination of interesting, innovative, and reliable laboratory exercises. This proceedings volume contains 10 papers: "Testing Issues of Foraging and Flocking Behavior" (C. C.…

  20. Using Focused Laboratory Management and Quality Improvement Projects to Enhance Resident Training and Foster Scholarship

    Science.gov (United States)

    Ford, Bradley A.; Klutts, J. Stacey; Jensen, Chris S.; Briggs, Angela S.; Robinson, Robert A.; Bruch, Leslie A.; Karandikar, Nitin J.

    2017-01-01

    Training in patient safety, quality, and management is widely recognized as an important element of graduate medical education. These concepts have been intertwined in pathology graduate medical education for many years, although training programs face challenges in creating explicit learning opportunities in these fields. Tangibly involving pathology residents in management and quality improvement projects has the potential to teach and reinforce key concepts and further fulfill Accreditation Council for Graduate Medical Education goals for pursuing projects related to patient safety and quality improvement. In this report, we present our experience at a pathology residency program (University of Iowa) in engaging pathology residents in projects related to practical issues of laboratory management, process improvement, and informatics. In this program, at least 1 management/quality improvement project, typically performed during a clinical chemistry/management rotation, was required and ideally resulted in a journal publication. The residency program also initiated a monthly management/informatics series for pathology externs, residents, and fellows that covers a wide range of topics. Since 2010, all pathology residents at the University of Iowa have completed at least 1 management/quality improvement project. Many of the projects involved aspects of laboratory test utilization, with some projects focused on other areas such as human resources, informatics, or process improvement. Since 2012, 31 peer-reviewed journal articles involving effort from 26 residents have been published. Multiple projects resulted in changes in ongoing practice, particularly within the hospital electronic health record. Focused management/quality improvement projects involving pathology residents can result in both meaningful quality improvement and scholarly output. PMID:28913416

  1. Using Focused Laboratory Management and Quality Improvement Projects to Enhance Resident Training and Foster Scholarship.

    Science.gov (United States)

    Krasowski, Matthew D; Ford, Bradley A; Klutts, J Stacey; Jensen, Chris S; Briggs, Angela S; Robinson, Robert A; Bruch, Leslie A; Karandikar, Nitin J

    2017-01-01

    Training in patient safety, quality, and management is widely recognized as an important element of graduate medical education. These concepts have been intertwined in pathology graduate medical education for many years, although training programs face challenges in creating explicit learning opportunities in these fields. Tangibly involving pathology residents in management and quality improvement projects has the potential to teach and reinforce key concepts and further fulfill Accreditation Council for Graduate Medical Education goals for pursuing projects related to patient safety and quality improvement. In this report, we present our experience at a pathology residency program (University of Iowa) in engaging pathology residents in projects related to practical issues of laboratory management, process improvement, and informatics. In this program, at least 1 management/quality improvement project, typically performed during a clinical chemistry/management rotation, was required and ideally resulted in a journal publication. The residency program also initiated a monthly management/informatics series for pathology externs, residents, and fellows that covers a wide range of topics. Since 2010, all pathology residents at the University of Iowa have completed at least 1 management/quality improvement project. Many of the projects involved aspects of laboratory test utilization, with some projects focused on other areas such as human resources, informatics, or process improvement. Since 2012, 31 peer-reviewed journal articles involving effort from 26 residents have been published. Multiple projects resulted in changes in ongoing practice, particularly within the hospital electronic health record. Focused management/quality improvement projects involving pathology residents can result in both meaningful quality improvement and scholarly output.

  2. Laboratory measurements of immersion freezing abilities of non-proteinaceous and proteinaceous biological particulate proxies

    Science.gov (United States)

    Cory, K.; Tobo, Y.; Murata, K.; Whiteside, C. L.; McCauley, B.; Bouma, C.; Hiranuma, N.

    2017-12-01

    Non-proteinaceous and proteinaceous biological aerosols are abundant within the atmosphere and have the potential to impact the climate through cloud and precipitation formation. In this study, we present the differences in the laboratory-measured freezing capabilities of the non-proteinaceous and proteinaceous biological materials to determine which has more potential to impact the ice nucleation in the clouds. As non-proteinaceous surrogates, we examined multiple cellulose materials (e.g., microcrystalline and nanocrystalline cellulose) whose sizes range from 100 nm to >100 μm (according to manufacturer report). For proteinaceous proxies, we looked at different gram-negative bacteria, such as Pseudamonas aeruginosa, Escherichia coli, Serratia marcescens, Citrobacter freundii, and Snomax, (which contains P. syringae) that can be found around the proximity of the Texas Panhandle. By using the Cryogenic Refrigeration Applied Freezing Test (CRAFT) system, we estimated immersion freezing efficiency (i.e., ice nucleation activity scaled to a unit of mass) of each sample at the temperatures greater than -30°C. We have observed that not all gram-negative bacteria has high immersion freezing activity, but the few do have a warmer temperature onset (>-20 °C) than the cellulose used. For those that did not exhibit substantial freezing efficiencies, they had similar freezing properties as the broth, in which the bacteria were incubated, as well as the cellulose materials examined. These observations suggest the presence and potential importance of bacterial cellulose in the atmospheric ice nucleation. From here, we need to conduct more in-depth investigation in the effects of a wider variety of atmospherically relevant biological aerosols to get a better understanding of the effects of said aerosols on overall aerosol-cloud interactions. Acknowledgments: K. Cory would like to acknowledge NSF-EAPSI and JSPS Summer Program for the travel fellowship support. N. Hiranuma

  3. Automation of the National Water Quality Laboratories, U. S. Geological Survey. I. Description of laboratory functions and definition of the automation project

    Energy Technology Data Exchange (ETDEWEB)

    Morris, W.F.; Ames, H.S.

    1977-07-01

    In January 1976, the Water Resources Division of the U.S. Geological Survey asked Lawrence Livermore Laboratory to conduct a feasibility study for automation of the National Water Quality (NWQ) Laboratory in Denver, Colorado (formerly Denver Central Laboratory). Results of the study were published in the Feasibility Study for Automation of the Central Laboratories, Lawrence Livermore Laboratory, Rept. UCRL-52001 (1976). Because the present system for processing water samples was found inadequate to meet the demands of a steadily increasing workload, new automation was recommended. In this document we present details necessary for future implementation of the new system, as well as descriptions of current laboratory automatic data processing and analytical facilities to better define the scope of the project and illustrate what the new system will accomplish. All pertinent inputs, outputs, and other operations that define the project are shown in functional designs.

  4. Automation of the National Water Quality Laboratories, U.S. Geological Survey. I. Description of laboratory functions and definition of the automation project

    International Nuclear Information System (INIS)

    Morris, W.F.; Ames, H.S.

    1977-01-01

    In January 1976, the Water Resources Division of the U.S. Geological Survey asked Lawrence Livermore Laboratory to conduct a feasibility study for automation of the National Water Quality (NWQ) Laboratory in Denver, Colorado (formerly Denver Central Laboratory). Results of the study were published in the Feasibility Study for Automation of the Central Laboratories, Lawrence Livermore Laboratory, Rept. UCRL-52001 (1976). Because the present system for processing water samples was found inadequate to meet the demands of a steadily increasing workload, new automation was recommended. In this document we present details necessary for future implementation of the new system, as well as descriptions of current laboratory automatic data processing and analytical facilities to better define the scope of the project and illustrate what the new system will accomplish. All pertinent inputs, outputs, and other operations that define the project are shown in functional designs

  5. Inter-laboratory project q calibration of SANS instruments using silver behenate

    International Nuclear Information System (INIS)

    Ikram, Abarrul; Gunawan; Edy Giri, Putra; Suzuki, Jun-ichi; Knott, Robert

    2000-01-01

    The inter-laboratory project for q-calibration of SANS (small angle neutron scattering) using silver behenate was carried out among Indonesia National Nuclear Energy Agency (BATAN), Japan Atomic Energy Research Institute (JAERI) and Australian Nuclear Science and Technology Organization (ANSTO). The standard sample of silver behenate, [CH 3 (CH 2 ) 20 COOAg](AgBE), has been assessed as an international standard for the calibration of both x-ray and neutron scattering instruments. The results indicate excellent agreement for q calibration obtained among the three laboratories, BATAN, JAERI and ANSTO. (Y. Kazumata)

  6. Shield calculation of project for instrument calibration integrated laboratory of IPEN-Sao Paulo, Brazil

    International Nuclear Information System (INIS)

    Barros, Gustavo A.S.J.; Caldas, Linda V.E.

    2009-01-01

    This work performed the shield calculation of the future rooms walls of the five X-ray equipment of the Instrument Calibration Laboratory of the IPEN, Sao Paulo, Brazil, which will be constructed in project of laboratory enlargement. The obtained results by application of a calculation methodology from an international regulation have shown that the largest thickness of shielding (25.7 cm of concrete or 7.1 mm of lead) will be of the wall which will receive the primary beam of the equipment with a 320 kV voltage. The cost/benefit analysis indicated the concrete as the best material option for the shielding

  7. Environmental setting for biological variability at PTEPBN project of West Kalimantan

    International Nuclear Information System (INIS)

    Suwadji, E.; Endrawanto

    1995-01-01

    Biological variability was needed in the arrangement of environmental evaluation study on term of environmental impact assessment. The activity was carried out at PTEPBN project to find out and to predict the environmental setting of outgoing and ongoing project as well as the project operational after post construction. Methods to find out the environmental setting on biological variability were proposed. Based on the observation data on its terrestrial and aquatic flora and fauna, it can be concluded that terrestrial flora was found at fair to good value, terrestrial fauna at fair to good whereas aquatic flora and fauna at good. (author). 8 refs, 7 tabs, 1 fig

  8. Looking through the Lens: Adapting and Modifying Photovoice Projects for Active Learning and Engagement in Biology

    OpenAIRE

    Karobi Moitra

    2016-01-01

    Photovoice projects traditionally include original visual imagery and minimal text to tell a powerful story. Photovoice student projects have been utilized in the health and social sciences to involve students with the local community and also in community-based projects to help at-risk youth call attention to their community problems, such as disease and drug use. The tool of the photovoice can be used in the biology classroom to engage students in the active learning process through communi...

  9. SHynergie: Development of a virtual project laboratory for monitoring hydraulic stimulations

    Science.gov (United States)

    Renner, Jörg; Friederich, Wolfgang; Meschke, Günther; Müller, Thomas; Steeb, Holger

    2016-04-01

    Hydraulic stimulations are the primary means of developing subsurface reservoirs regarding the extent of fluid transport in them. The associated creation or conditioning of a system of hydraulic conduits involves a range of hydraulic and mechanical processes but also chemical reactions, such as dissolution and precipitation, may affect the stimulation result on time scales as short as hours. In the light of the extent and complexity of these processes, the steering potential for the operator of a stimulation critically depends on the ability to integrate the maximum amount of site-specific information with profound process understanding and a large spectrum of experience. We report on the development of a virtual project laboratory for monitoring hydraulic stimulations within the project SHynergie (http://www.ruhr-uni-bochum.de/shynergie/). The concept of the laboratory envisioned product that constitutes a preparing and accompanying rather than post-processing instrument ultimately accessible to persons responsible for a project over a web-repository. The virtual laboratory consists of a data base, a toolbox, and a model-building environment. Entries in the data base are of two categories. On the one hand, selected mineral and rock properties are provided from the literature. On the other hand, project-specific entries of any format can be made that are assigned attributes regarding their use in a stimulation problem at hand. The toolbox is interactive and allows the user to perform calculations of effective properties and simulations of different types (e.g., wave propagation in a reservoir, hydraulic test). The model component is also hybrid. The laboratory provides a library of models reflecting a range of scenarios but also allows the user to develop a site-specific model constituting the basis for simulations. The laboratory offers the option to use its components following the typical workflow of a stimulation project. The toolbox incorporates simulation

  10. Idaho National Laboratory Ten-Year Site Plan Project Description Document

    Energy Technology Data Exchange (ETDEWEB)

    Not Listed

    2012-03-01

    This document describes the currently active and proposed infrastructure projects listed in Appendix B of the Idaho National Laboratory 2013-2022 Ten Year Site Plan (DOE/ID-11449). It was produced in accordance with Contract Data Requirements List I.06. The projects delineated in this document support infrastructure needs at INL's Research and Education Campus, Materials and Fuels Complex, Advanced Test Reactor Complex and the greater site-wide area. The projects provide critical infrastructure needed to meet current and future INL opereational and research needs. Execution of these projects will restore, rebuild, and revitalize INL's physical infrastructure; enhance program execution, and make a significant contribution toward reducing complex-wide deferred maintenance.

  11. The Earth Microbiome Project and Global Systems Biology

    Energy Technology Data Exchange (ETDEWEB)

    Gilbert, Jack A.; Jansson, Janet K.; Knight, Rob

    2018-04-10

    Recently, we published the first large-scale analysis of data from the Earth Microbiome Project (1, 2), a truly multidisciplinary research program involving more than 500 scientists and 27,751 samples acquired from 43 countries. These samples represent myriad specimen types and span a wide range of biotic and abiotic factors, geographic locations, and physicochemical properties. The database (https://qiita.ucsd.edu/emp/) is still growing, with over 90,000 amplicon datasets, >500 metagenomic runs, and metabolomics datasets from a similar number of samples. Importantly, the techniques, data and analytical tools are all standardized and publicly accessible, providing a framework to support research at a scale of integration that just 7 years ago seemed impossible.

  12. Scientific projection paper on biologic effects of ionizing radiation

    International Nuclear Information System (INIS)

    Matanoski, G.

    1980-01-01

    There is widespread knowledge about the effects of radiation in human populations but the studies have had some limitations which have left gaps in our knowledge. Most populations have had exposure to high doses with little information on the effect of dose rate. The characteristics of the populations have been restricted by the location of the disaster, the occupational limitations, or the basic risks associated with the under-lying disease for which radiation was given. All doses have been estimated and such values are subject to marked variability particularly when they rely on sources of data such as hospital records. The biological data although extensive have several deficits in information. Which are the sites in which cancer is produced by irradiation and what are the cell types which are produced. The sensitivity of various tissues and organs are not similar and it is important to rank them according to susceptibility. This has been done in the past but the results are not complete for all cell types and organs. The temporal patterns for tumor development, the latent period, the period of expressed excess, the life-time risks need to be defined more precisely for the cancers. Many populations have not been followed long enough to express the complete risk

  13. Hawaii Geothermal Project annotated bibliography: Biological resources of the geothermal subzones, the transmission corridors and the Puna District, Island of Hawaii

    Energy Technology Data Exchange (ETDEWEB)

    Miller, S.E.; Burgett, J.M. [Fish and Wildlife Service, Honolulu, HI (United States). Pacific Islands Office

    1993-10-01

    Task 1 of the Hawaii Geothermal Project Interagency Agreement between the Fish and Wildlife Service and the Department of Energy-Oak Ridge National Laboratory (DOE) includes an annotated bibliography of published and unpublished documents that cover biological issues related to the lowland rain forest in Puna, adjacent areas, transmission corridors, and in the proposed Hawaii Geothermal Project (HGP). The 51 documents reviewed in this report cover the main body of biological information for these projects. The full table of contents and bibliography for each document is included along with two copies (as requested in the Interagency Agreement) of the biological sections of each document. The documents are reviewed in five main categories: (1) geothermal subzones (29 documents); (2) transmission cable routes (8 documents); (3) commercial satellite launching facility (Spaceport; 1 document); (4) manganese nodule processing facility (2 documents); (5) water resource development (1 document); and (6) ecosystem stability and introduced species (11 documents).

  14. Work plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-01

    The purpose of the Isotopes Facilities Deactivation Project (IFDP) is to place former isotopes production facilities at the Oak Ridge National Laboratory in a safe, stable, and environmentally sound condition; suitable for an extended period of minimum surveillance and maintenance (S&M) and as quickly and economical as possible. Implementation and completion of the deactivation project will further reduce the risks to the environment and to public safety and health. Furthermore, completion of the project will result in significant S&M cost savings in future years. The IFDP work plan defines the project schedule, the cost estimate, and the technical approach for the project. A companion document, the IFDP management plan, has been prepared to document the project objectives, define organizational relationships and responsibilities, and outline the management control systems to be employed in the management of the project. The project has adopted the strategy of deactivating the simple facilities first, to reduce the scope of the project and to gain experience before addressing more difficult facilities. A decision support system is being developed to identify the activities that best promote the project mission and result in the largest cost savings. This work plan will be reviewed and revised annually. Deactivation of IFDP facilities was initiated in FY 1994 and will be completed in FY 1999. The schedule for deactivation of facilities is shown. The total cost of the project is estimated to be $36M. The costs are summarized. Upon completion of deactivation, annual S&M costs of these facilities will be reduced from the current level of $5M per year to less than $1M per year.

  15. Work plan for the Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-08-01

    The purpose of the Isotopes Facilities Deactivation Project (IFDP) is to place former isotopes production facilities at the Oak Ridge National Laboratory in a safe, stable, and environmentally sound condition; suitable for an extended period of minimum surveillance and maintenance (S and M) and as quickly and economical as possible. Implementation and completion of the deactivation project will further reduce the risks to the environment and to public safety and health. Furthermore, completion of the project will result in significant S and M cost savings in future years. The IFDP work plan defines the project schedule, the cost estimate, and the technical approach for the project. A companion document, the EFDP management plan, has been prepared to document the project objectives, define organizational relationships and responsibilities, and outline the management control systems to be employed in the management of the project. The project has adopted the strategy of deactivating the simple facilities first, to reduce the scope of the project and to gain experience before addressing more difficult facilities. A decision support system is being developed to identify the activities that best promote the project mission and result in the largest cost savings. This work plan will be reviewed and revised annually. Deactivation of EFDP Facilities was initiated in FY 1994 and will be completed in FY 2000. The schedule for deactivation of facilities is shown. The total cost of the project is estimated to be $51M. The costs are summarized. Upon completion of deactivation, annual S and M costs of these facilities will be reduced from the current level of $5M per year to less than $1M per year

  16. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    Energy Technology Data Exchange (ETDEWEB)

    Bacri, C.O., E-mail: bacri@ipno.in2p3.f [Institut de Physique Nucleaire d' Orsay, 91406 Orsay Cedex, CNRS (UMR8608-IN2P3), Universite Paris-Sud (Paris XI) (France); Petitbon, V.; Pierre, S. [Institut de Physique Nucleaire d' Orsay, 91406 Orsay Cedex, CNRS (UMR8608-IN2P3), Universite Paris-Sud (Paris XI) (France)

    2010-02-11

    CACAO, Chimie des Actinides et Cibles radioActives a Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  17. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    Science.gov (United States)

    Bacri, C. O.; Petitbon, V.; Pierre, S.; Cacao Group

    2010-02-01

    CACAO, Chimie des Actinides et Cibles radioActives à Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  18. CACAO: A project for a laboratory for the production and characterization of thin radioactive layers

    International Nuclear Information System (INIS)

    Bacri, C.O.; Petitbon, V.; Pierre, S.

    2010-01-01

    CACAO, Chimie des Actinides et Cibles radioActives a Orsay (actinide chemistry and radioactive targets at Orsay), is a project under construction that consists of the installation of a hot laboratory dedicated to the production and characterization of thin radioactive layers. The project aims to be a joint CNRS-CEA national laboratory to overcome difficulties related mainly to safety issues and to the lack of knowledge and potential manpower. The first goal is to fulfill, at least, the needs of the whole French community, and to be able to coordinate the different activities related to radioactive targets. For this purpose, itis important to be complementary to already existing international installations. Inside this framework, it will of course be possible to produce and/or characterize targets for other users.

  19. Waste minimization value engineering workshop for the Los Alamos National Laboratory Omega West Reactor Decommissioning Project

    International Nuclear Information System (INIS)

    Hartnett, S.; Seguin, N.; Burns, M.

    1995-01-01

    The Los Alamos National Laboratory Pollution Prevention Program Office sponsored a Value Engineering (VE) Workshop to evaluate recycling options and other pollution prevention and waste minimization (PP/WMin) practices to incorporate into the decommissioning of the Omega West Reactor (OWR) at the laboratory. The VE process is an organized, systematic approach for evaluating a process or design to identify cost saving opportunities, or in this application, waste reduction opportunities. This VE Workshop was a facilitated process that included a team of specialists in the areas of decontamination, decommissioning, PP/WMin, cost estimating, construction, waste management, recycling, Department of Energy representatives, and others. The uniqueness of this VE Workshop was that it used an interdisciplinary approach to focus on PP/WMin practices that could be included in the OWR Decommissioning Project Plans and specifications to provide waste reduction. This report discusses the VE workshop objectives, summarizes the OWR decommissioning project, and describes the VE workshop activities, results, and lessons learned

  20. Horonobe Underground Research Laboratory project. Investigation report for the 2006 fiscal year

    International Nuclear Information System (INIS)

    Matsui, Hiroya; Nakayama, Masashi; Sanada, Hiroyuki

    2008-05-01

    The Horonobe Underground Research Laboratory is planned to extend over a period of 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations', 'Phase 2: Construction Phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the results of the investigations for the 2006 fiscal year (2006/2007), the second year of the Phase 2 investigations. The investigations, which are composed of 'Geoscientific research' and 'R and D on the geological disposal of high-level radioactive waste (HLW)', were carried out according to 'Horonobe Underground Research Laboratory Project Investigation Program for the 2006 Fiscal Year'. The results of these investigations, along with the results which were obtained in other departments of Japan Atomic Energy Agency (JAEA), are properly offered to the implementations and the safety regulations. JAEA proceeded with the project in, collaboration with experts from domestic and overseas research organisation. (author)

  1. Waste minimization value engineering workshop for the Los Alamos National Laboratory Omega West Reactor Decommissioning Project

    Energy Technology Data Exchange (ETDEWEB)

    Hartnett, S.; Seguin, N. [Benchmark Environmental Corp., Albuquerque, NM (United States); Burns, M. [Los Alamos National Lab., NM (United States)

    1995-12-31

    The Los Alamos National Laboratory Pollution Prevention Program Office sponsored a Value Engineering (VE) Workshop to evaluate recycling options and other pollution prevention and waste minimization (PP/WMin) practices to incorporate into the decommissioning of the Omega West Reactor (OWR) at the laboratory. The VE process is an organized, systematic approach for evaluating a process or design to identify cost saving opportunities, or in this application, waste reduction opportunities. This VE Workshop was a facilitated process that included a team of specialists in the areas of decontamination, decommissioning, PP/WMin, cost estimating, construction, waste management, recycling, Department of Energy representatives, and others. The uniqueness of this VE Workshop was that it used an interdisciplinary approach to focus on PP/WMin practices that could be included in the OWR Decommissioning Project Plans and specifications to provide waste reduction. This report discusses the VE workshop objectives, summarizes the OWR decommissioning project, and describes the VE workshop activities, results, and lessons learned.

  2. Biological air contamination in elderly care centers: geria project.

    Science.gov (United States)

    Aguiar, Lívia; Mendes, Ana; Pereira, Cristiana; Neves, Paula; Mendes, Diana; Teixeira, João Paulo

    2014-01-01

    Indoor air quality (IAQ) affects health particularly in susceptible individuals such as the elderly. It has been estimated that the older population spends approximately 19-20 h/d indoors, and the majority of the elderly spend all of their time indoors in elderly care centers (ECC). Older individuals may be particularly at risk of exposure to detrimental effects from pollutants, even at low concentrations, due to common and multiple underlying chronic diseases that increase susceptibility. This study, aimed to assess the impact of indoor biological agents in 22 ECC located in Porto, was conducted during summer and winter from November 2011 to August 2013 at a total of 141 areas within dining rooms, drawing rooms, medical offices, and bedrooms (including the bedridden). Air sampling was carried out with a microbiological air sampler (Merck MAS-100) and using tryptic soy agar for bacteria and malt extract agar for fungi. The results obtained were compared with the recently revised Portuguese standards. In winter, mean fungi concentration exceeded reference values, while bacteria concentrations were within the new standards in both seasons. The main fungi species found indoors were Cladosporium (73%) in summer and Penicillium (67%) in winter. Aspergillus fumigatus, Aspergillus niger, and Aspergillus flavus, known potential pathogenic/toxigenic species, were also identified. Although the overall rate and mean values of bacteria and fungi found in ECC indoor air met Portuguese legislation, some concern is raised by the presence of pathogenic microorganisms. Simple measures, like opening windows and doors to promote air exchange and renewal, may improve effectiveness in enhancing IAQ.

  3. Research and Development Project Selection Methods at the Air Force Wright Aeronautical Laboratories.

    Science.gov (United States)

    1985-09-01

    personal and telephone interviews. Ten individuals from each of the four AFWAL Laboratories were interrviewed. The results illustrated that few of the...680). Aaker and Tyebee. 1978. The authors constructed a model that dealt with the selection of interdependent R&D projects. The model covers three...of this research effort. Scope * The data collection method used in this study consisted of a combination of personal and telephone interviews. The

  4. Biological in situ treatment of soil contaminated with petroleum - Laboratory scale simulations

    International Nuclear Information System (INIS)

    Palvall, B.

    1997-06-01

    Laboratory scale simulations of biological in situ treatment of soil contaminated with petroleum compounds have been made in order to get a practical concept in the general case. The work was divided into seven distinct parts. Characterisation, leaching tests and introductory microbiological investigations were followed by experiments in suspended phases and in situ simulations of solid phase reactors. For the suspensions, ratios L/S 3/1 and shaking for a couple of hours were enough to detach organic compounds in colloid or dissolved form. When testing for a time of one month anaerobic environment and cold temperatures of 4 centigrade as well gave acceptable reductions of the actual pollution levels. The range of variation in the soil tests performed showed that at least triple samples are needed to get satisfactory statistical reliability. It was shown that adequate experimental controls demand very high concentrations of e.g. sodium azide when dealing with soil samples. For triple samples in suspended phase without inoculation the weight ratios of oxygen consumption/biological degradation of aliphatic compounds were 2.41 to 2.96. For the complex overall reduction no exact rate constants could be found. The reduction of hydrocarbons were in the interval 27 to 95 % in suspension tests. Solid phase simulations with maximum water saturation showed the highest degree of reduction of hydrocarbons when using dissolved peroxide of hydrogen as electron acceptor while the effect of an active sludge reactor in series was little - reductions of aliphatic compounds were between 21 and 33 % and of aromatic compounds between 32 and 65 %. The influence of different contents of water was greater than adding inoculum or shaking the soil at different intervals in the unsaturated cylinders. The starting level of hydrocarbons was 2400 mg/kg dry weight soil and the end analyses were made after 100 days. The reduction was between 32 and 80 %. 82 refs

  5. Synthesized research report in the second mid-term research phase. Mizunami Underground Research Laboratory project, Horonobe Underground Research Laboratory project and geo-stability project

    International Nuclear Information System (INIS)

    Hama, Katsuhiro; Sasao, Eiji; Iwatsuki, Teruki; Saegusa, Hiromitsu; Sato, Toshinori; Umeda, Koji; Yasue, Kenichi; Asamori, Koichi; Osawa, Hideaki; Koide, Kaoru; Nagae, Isako; Natsuyama, Ryoko; Mizuno, Takashi; Fujita, Tomoo; Sasamoto, Hiroshi; Matsuoka, Toshiyuki; Yokota, Hideharu; Ishii, Eiichi; Aoyagi, Kazuhei; Nakayama, Masashi; Ito, Hiroaki; Tsusaka, Kimikazu; Ohyama, Takuya; Senba, Takeshi; Amano, Kenji

    2015-08-01

    We have synthesised the research results from Mizunami/Horonobe URLs and geo-stability projects in the second mid-term research phase. It could be used as technical bases for NUMO/Regulator in each decision point from siting to beginning of disposal (Principal Investigation to Detailed Investigation Phase). High quality construction techniques and field investigation methods have been developed and implemented and these will be directly applicable to the National Disposal Program (along with general assessments of hazardous natural events and processes). It will be crucial to acquire technical knowledge on decisions of partial backfilling and final closure by actual field experiments in Mizunami/Horonobe URLs as main themes for the next phases. (author)

  6. The Protein Information Management System (PiMS): a generic tool for any structural biology research laboratory.

    Science.gov (United States)

    Morris, Chris; Pajon, Anne; Griffiths, Susanne L; Daniel, Ed; Savitsky, Marc; Lin, Bill; Diprose, Jonathan M; da Silva, Alan Wilter; Pilicheva, Katya; Troshin, Peter; van Niekerk, Johannes; Isaacs, Neil; Naismith, James; Nave, Colin; Blake, Richard; Wilson, Keith S; Stuart, David I; Henrick, Kim; Esnouf, Robert M

    2011-04-01

    The techniques used in protein production and structural biology have been developing rapidly, but techniques for recording the laboratory information produced have not kept pace. One approach is the development of laboratory information-management systems (LIMS), which typically use a relational database schema to model and store results from a laboratory workflow. The underlying philosophy and implementation of the Protein Information Management System (PiMS), a LIMS development specifically targeted at the flexible and unpredictable workflows of protein-production research laboratories of all scales, is described. PiMS is a web-based Java application that uses either Postgres or Oracle as the underlying relational database-management system. PiMS is available under a free licence to all academic laboratories either for local installation or for use as a managed service.

  7. Project plan for the decontamination and decommissioning of the Argonne National Laboratory Experimental Boiling Water Reactor

    International Nuclear Information System (INIS)

    Boing, L.E.

    1989-12-01

    In 1956, the Experimental Boiling Water Reactor (EBWR) Facility was first operated at Argonne National Laboratory (ANL) as a test reactor to demonstrate the feasibility of operating an integrated power plant using a direct cycle boiling water reactor as a heat source. In 1967, ANL permanently shut down the EBWR and placed it in dry lay-up. This project plan presents the schedule and organization for the decontamination and decommissioning of the EBWR Facility which will allow it to be reused by other ANL scientific research programs. The project total estimated cost is $14.3M and is projected to generate 22,000 cubic feet of low-level radioactive waste which will be disposed of at an approved DOE burial ground. 18 figs., 3 tabs

  8. Los Alamos National Laboratory Environmental Restoration Project quarterly technical report, April--June 1994

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-18

    This quarterly report describes the technical status of activities in the Los Alamos National Laboratory Environmental Restoration (ER) Project. Each activity is identified by an activity data sheet number, a brief title describing the activity or the technical area where the activity is located, and the name of the project leader. The Hazardous and Solid Waste Amendments (HSWA) portion of the facility operating permit requires the submission of a technical progress report on a quarterly basis. This report, submitted to fulfill the permit`s requirement, summarizes the work performed and the results of sampling and analysis in the ER Project. Suspect waste found include: Radionuclides, high explosives, metals, solvents and organics. The data provided in this report have not been validated. These data are considered ``reviewed data.``

  9. Project plan for the decontamination and decommissioning of the Argonne National Laboratory Experimental Boiling Water Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Boing, L.E.

    1989-12-01

    In 1956, the Experimental Boiling Water Reactor (EBWR) Facility was first operated at Argonne National Laboratory (ANL) as a test reactor to demonstrate the feasibility of operating an integrated power plant using a direct cycle boiling water reactor as a heat source. In 1967, ANL permanently shut down the EBWR and placed it in dry lay-up. This project plan presents the schedule and organization for the decontamination and decommissioning of the EBWR Facility which will allow it to be reused by other ANL scientific research programs. The project total estimated cost is $14.3M and is projected to generate 22,000 cubic feet of low-level radioactive waste which will be disposed of at an approved DOE burial ground. 18 figs., 3 tabs.

  10. Mizunami Underground Research Laboratory project. Annual report for fiscal year 2007

    International Nuclear Information System (INIS)

    Nishio, Kazuhisa; Matsuoka, Toshiyuki; Tsuruta, Tadahiko; Amano, Kenji; Ohyama, Takuya; Takeuchi, Ryuji; Saegusa, Hiromitsu; Hama, Katsuhiro; Mizuno, Takashi; Sai, Masataka; Hirano, Toru; Iyatomi, Yosuke; Shimada, Akiomi; Matsui, Hiroya; Ogata, Nobuhisa; Uchida, Masahiro; Sugihara, Kozo; Mikake, Shinichiro; Ikeda, Koki; Yamamoto, Masaru

    2009-03-01

    Japan Atomic Energy Agency (JAEA) at Tono Geoscience Center (TGC) is developing a geoscientific research project named Mizunami Underground Research Laboratory (MIU) Project in crystalline rock environment in order to establish scientific and technological basis for geological disposal of HLW. Geoscientific research at MIU is planned to be carried out in three phases over a period of 20 years; Surface-based Investigation Phase (Phase I), Construction Phase (Phase II) and Operation Phase (Phase III). Currently, the project is under the Construction Phase. This document presents the following results of the research and development performed in fiscal year 2007, as a part of the Construction Phase based on the MIU Master Plan updated in 2002, 1) Investigation at the MIU Construction Site and the Shobasama Site, 2) Construction at the MIU Construction Site, 3) Research Collaboration. (author)

  11. Mizunami Underground Research Laboratory project. Annual report for fiscal year 2005

    International Nuclear Information System (INIS)

    Nishio, Kazuhisa; Matsuoka, Toshiyuki; Tsuruta, Tadahiko; Amano, Kenji; Ohyama, Takuya; Takeuchi, Ryuji; Saegusa, Hiromitsu; Hama, Katsuhiro; Mizuno, Takashi; Sai, Masataka; Iyatomi, Yosuke; Shimada, Akiomi; Ogata, Nobuhisa; Uchida, Masahiro; Sugihara, Kozo; Mikake, Shinichiro; Ikeda, Koki; Yamamoto, Masaru; Yoshida, Haruo; Nakama, Shigeo; Seno, Yasuhiro; Kuroda, Hidetaka; Semba, Takeshi

    2009-03-01

    Japan Atomic Energy Agency (JAEA) at Tono Geoscience Center (TGC) is developing a geoscientific research project named Mizunami Underground Research Laboratory (MIU) project in crystalline rock environment in order to establish scientific and technological basis for geological disposal of HLW. Geoscientific research at MIU is planned to be carried out in three phases over a period of 20 years; Surface-based Investigation Phase (Phase 1), Construction Phase (Phase 2) and Operation Phase (Phase 3). Currently, the project is under the Construction Phase. This document presents the following results of the research and development performed in 2005 fiscal year, as a part of the Construction Phase based on the MIU Master Plan updated in 2002, 1) Investigation at the MIU Construction Site and the Shobasama Site, 2) Construction at the MIU Construction Site, 3) Research Collaboration. (author)

  12. Final report for the protocol extensions for ATM Security Laboratory Directed Research and Development Project

    Energy Technology Data Exchange (ETDEWEB)

    Tarman, T.D.; Pierson, L.G.; Brenkosh, J.P. [and others

    1996-03-01

    This is the summary report for the Protocol Extensions for Asynchronous Transfer Mode project, funded under Sandia`s Laboratory Directed Research and Development program. During this one-year effort, techniques were examined for integrating security enhancements within standard ATM protocols, and mechanisms were developed to validate these techniques and to provide a basic set of ATM security assurances. Based on our experience during this project, recommendations were presented to the ATM Forum (a world-wide consortium of ATM product developers, service providers, and users) to assist with the development of security-related enhancements to their ATM specifications. As a result of this project, Sandia has taken a leading role in the formation of the ATM Forum`s Security Working Group, and has gained valuable alliances and leading-edge experience with emerging ATM security technologies and protocols.

  13. Mizunami Underground Research Laboratory project. Annual report for fiscal year 2008

    International Nuclear Information System (INIS)

    Takeuchi, Shinji; Kunimaru, Takanori; Nishio, Kazuhisa; Tsuruta, Tadahiko; Matsuoka, Toshiyuki; Hayano, Akira; Takeuchi, Ryuji; Saegusa, Hiromitsu; Ohyama, Takuya; Mizuno, Takashi; Hirano, Toru; Ogata, Nobuhisa; Hama, Katsuhiro; Iyatomi, Yosuke; Shimada, Akiomi; Matsui, Hiroya; Ito, Hiroaki; Sugihara, Kozo; Mikake, Shinichiro; Ikeda, Koki; Yamamoto, Masaru

    2010-07-01

    Japan Atomic Energy Agency (JAEA) at Tono Geoscience Center (TGC) is developing a geoscientific research project named Mizunami Underground Research Laboratory (MIU) Project in crystalline rock environment in order to establish scientific and technological basis for geological disposal of HLW. Geoscientific research at MIU is planned to be carried out in three phases over a period of 20 years; Surface-based Investigation Phase (Phase 1), Construction Phase (Phase 2) and Operation Phase (Phase 3). Currently, the project is under the Construction Phase. This document presents the following results of the research and development performed in fiscal year 2008, as a part of the Construction Phase based on the MIU Master Plan updated in 2002, 1) Investigation at the MIU Construction Site and the Shobasama Site, 2) Construction at the MIU Construction Site, 3) Research Collaboration. (author)

  14. Biology and bionomics of dysdercus koenigii f. (hemiptera: pyrrhocoridae) under laboratory conditions

    International Nuclear Information System (INIS)

    Jaleel, W.; Naqqash, M.N.

    2013-01-01

    Red Cotton Bug, Dysdercus koenigii F., (Hemiptera: Pyrrhocoridae) is an important pest of cotton in South East Asia. Studies were carried out during 2012 to find the effect of temperature on incubation period and to explore the reproductive biology and bionomics of D. koenigii under laboratory conditions. Minimum incubation period (4.70+-0.42 days) was recorded at 35 degree C while the eggs failed to hatch at 40 degree C at 70-75% relative humidity. There were five nymphal instars which completed their development in 23.42+-2.49 days. The female lived longer (20.85+-6.12 days) than the male (16.18+-6.06 days). Each female mated three times in her life and there was statistically significant difference in mating duration (days), number of eggs laid and hatching percentage in each mating time. Number of eggs and hatching percentage was significantly higher after 1st time mating followed by 2nd and 3rd time matings. Duration of pre-oviposition, oviposition and post oviposition period recorded was 7.47+-0.86, 12.43+-0.82 and 8.77+-2.41 days, respectively. The study will help in devising pest management strategy against D. koenigii. (author)

  15. Laboratory Study on Biological Control of Ticks (Acari: Ixodidae by Entomopathogenic Indigenous Fungi (Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    M Abdigoudarzi

    2009-12-01

    Full Text Available Background: Chemical control method using different acaricides as spray, dipping solution or pour-on is routinely used for controlling ticks. Biological control agents are favorable due to their safety for animals and environment. Entomopathogenic fungi such as Beauveria bassiana are well known for controlling ticks. In this study, two Iranian indigenous strains of B. bassiana (B. bassiana 5197 and B. bassiana Evin were selected and grown on specific me­dia. The pathogenic effects of these strains were evaluated on adult stages of two Iranian Ixodidae members (H. anatolicum anatolicum Koch 1844, and H. marginatum Koch 1844 by dipping method.Methods: Two Iranian strains of Beauveria bassiana (Beauveria bassiana 5197 and Beauveria bassiana Evin were selected and were grown successfully on specific media. The pathogenic effects of these strains were evaluated on adult stages of Iranian Ixodidae members such as, Hyalomma anatolicum anatolicum and H. marginatum by dipping method (these ticks were grown up at laboratory conditions during 2002 up to 2003 and still it is continued .Results: There was no effect of strain 5197 on mortality or fecundity rates for ticks. There was acute phase sign of paralysis in test group after dipping ticks in suspension made from Evin strain of B. bassiana. In addition, the test groups were totally died after four months, but the control groups survived for six months.Conclusion: High concentration of fungal spores is needed for inducing fungal infection. Additional study using different strains and fungi on Iranian ticks is proposed. 

  16. Biological and Environmental Research Program at Oak Ridge National Laboratory, FY 1992--1994

    International Nuclear Information System (INIS)

    1992-01-01

    This report is the 1992--1994 Program Director's Overview Report for Oak Ridge National Laboratory's (ORNL's) Biological and Environmental Research (BER) Program, and as such it addresses KP-funded work at ORNL conducted during FY 1991 and in progress during FY 1992; it also serves as a planning document for the remainder of FY 1992 through FY 1994. Non-BER funded work at ORNL relevant to the mission of OHER is also discussed. The second section of the report describes ORNL facilities and resources used by the BER program. The third section addresses research management practices at ORNL. The fourth, fifth, and sixth sections address BER-funded research in progress, program accomplishments and research highlights, and program orientation for the remainder of FY 1992 through FY 1994, respectively. Work for non-BER sponsors is described in the seventh section, followed by a discussion of significant near and long-term issues facing BER work at ORNL in the eighth section. The last section provides a statistical summary of BER research at ORNL. Appendices supplement the above topics with additional detail

  17. Biological and Environmental Research Program at Oak Ridge National Laboratory, FY 1992--1994

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    This report is the 1992--1994 Program Director's Overview Report for Oak Ridge National Laboratory's (ORNL's) Biological and Environmental Research (BER) Program, and as such it addresses KP-funded work at ORNL conducted during FY 1991 and in progress during FY 1992; it also serves as a planning document for the remainder of FY 1992 through FY 1994. Non-BER funded work at ORNL relevant to the mission of OHER is also discussed. The second section of the report describes ORNL facilities and resources used by the BER program. The third section addresses research management practices at ORNL. The fourth, fifth, and sixth sections address BER-funded research in progress, program accomplishments and research highlights, and program orientation for the remainder of FY 1992 through FY 1994, respectively. Work for non-BER sponsors is described in the seventh section, followed by a discussion of significant near and long-term issues facing BER work at ORNL in the eighth section. The last section provides a statistical summary of BER research at ORNL. Appendices supplement the above topics with additional detail.

  18. Preliminary report of biological intrusion studies at the Idaho National Engineering Laboratory subsurface disposal area

    International Nuclear Information System (INIS)

    Reynolds, T.D.; Arthur, W.J.

    1983-01-01

    As part of a larger study on the effects of biological intrusion of plants and animals into the soil cover placed over low-level radioactive wastes stored at the Idaho National Engineering Laboratory Subsurface Disposal Area (SDA), research was initiated in the summer of 1982 to determine the burrow characteristics and movement patterns of several small mammal species, and the rooting depths of various plants. The depth, length, and volume of burrows were determined for four small mammal species: deer mouse (Peromyscus maniculatus), Ord's kangaroo rat (Dipodomys ordii), montane vole (Microtus montanus), and Townsend's ground squirrel (Spermophilis townsendii). The latter species excavated the greatest mean burrow depth (39 cm), length (404 cm), and volume (14.8 1). Movement patterns of three species were determined by radiotelemetry. The mean area of use for P. maniculatus, D. ordii, and M. montanus was 2.3, 1.5, and 1.2 ha respectively. Limited data on rooting depths of various native and introduced plant species at the SDA were obtained by literature review and excavation. During FY-83, experiments will be conducted, using the information obtained from the first year of this study, to evaluate the impact of burrowing mammals and root intrusion on the integrity of the soil cover currently existing at the SDA. Details of these experimental studies are presented

  19. Decontamination and decommissioning of the Experimental Boiling Water Reactor (EBWR): Project final report, Argonne National Laboratory

    International Nuclear Information System (INIS)

    Fellhauer, C.R.; Boing, L.E.; Aldana, J.

    1997-03-01

    The Final Report for the Decontamination and Decommissioning (D ampersand D) of the Argonne National Laboratory - East (ANL-E) Experimental Boiling Water Reactor (EBWR) facility contains the descriptions and evaluations of the activities and the results of the EBWR D ampersand D project. It provides the following information: (1) An overall description of the ANL-E site and EBWR facility. (2) The history of the EBWR facility. (3) A description of the D ampersand D activities conducted during the EBWR project. (4) A summary of the final status of the facility, including the final and confirmation surveys. (5) A summary of the final cost, schedule, and personnel exposure associated with the project, including a summary of the total waste generated. This project report covers the entire EBWR D ampersand D project, from the initiation of Phase I activities to final project closeout. After the confirmation survey, the EBWR facility was released as a open-quotes Radiologically Controlled Area,close quotes noting residual elevated activity remains in inaccessible areas. However, exposure levels in accessible areas are at background levels. Personnel working in accessible areas do not need Radiation Work Permits, radiation monitors, or other radiological controls. Planned use for the containment structure is as an interim transuranic waste storage facility (after conversion)

  20. Evaluation of an LED Retrofit Project at Princeton University’s Carl Icahn Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Robert G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Murphy, Arthur L. [Princeton Univ., NJ (United States); Perrin, Tess E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-11-25

    The LED lighting retrofit at the Carl Icahn Laboratory of the Lewis-Sigler Institute for Integrative Genomics was the first building-wide interior LED project at Princeton University, following the University’s experiences from several years of exterior and small-scale interior LED implementation projects. The project addressed three luminaire types – recessed 2x2 troffers, cove and other luminaires using linear T8 fluorescent lamps, and CFL downlights - which combined accounted for over 564,000 kWh of annual energy, over 90% of the lighting energy used in the facility. The Princeton Facilities Engineering staff used a thorough process of evaluating product alternatives before selecting an acceptable LED retrofit solution for each luminaire type. Overall, 815 2x2 luminaires, 550 linear fluorescent luminaires, and 240 downlights were converted to LED as part of this project. Based solely on the reductions in wattage in converting from the incumbent fluorescent lamps to LED retrofit kits, the annual energy savings from the project was over 190,000 kWh, a savings of 37%. An additional 125,000 kWh of energy savings is expected from the implementation of occupancy and task-tuning control solutions, which will bring the total savings for the project to 62%.

  1. Ethical and methodological standards for laboratory and medical biological rhythm research.

    Science.gov (United States)

    Portaluppi, Francesco; Touitou, Yvan; Smolensky, Michael H

    2008-11-01

    The main objectives of this article are to update the ethical standards for the conduct of human and animal biological rhythm research and recommend essential elements for quality chronobiological research information, which should be especially useful for new investigators of the rhythms of life. A secondary objective is to provide for those with an interest in the results of chronobiology investigations, but who might be unfamiliar with the field, an introduction to the basic methods and standards of biological rhythm research and time series data analysis. The journal and its editors endorse compliance of all investigators to the principles of the Declaration of Helsinki of the World Medical Association, which relate to the conduct of ethical research on human beings, and the Guide for the Care and Use of Laboratory Animals of the Institute for Laboratory Animal Research of the National Research Council, which relate to the conduct of ethical research on laboratory and other animals. The editors and the readers of the journal expect the authors of submitted manuscripts to have adhered to the ethical standards dictated by local, national, and international laws and regulations in the conduct of investigations and to be unbiased and accurate in reporting never-before-published research findings. Authors of scientific papers are required to disclose all potential conflicts of interest, particularly when the research is funded in part or in full by the medical and pharmaceutical industry, when the authors are stock-holders of the company that manufactures or markets the products under study, or when the authors are a recent or current paid consultant to the involved company. It is the responsibility of the authors of submitted manuscripts to clearly present sufficient detail about the synchronizer schedule of the studied subjects (i.e., the sleep-wake schedule, ambient light-dark cycle, intensity and spectrum of ambient light exposure, seasons when the research was

  2. Collection of URL measurement data in 2010 fiscal year at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Inagaki, Daisuke; Sawada, Sumiyuki; Tokiwa, Tetsuya; Tsusaka, Kimikazu; Amano, Yuki; Niinuma, Hiroaki

    2012-09-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The URL project consists of two major research areas, 'Geoscientific Research' and 'R and D on Geological Disposal Technologies', and proceeds in three overlapping phases, 'Phase I: Surface-based investigations', 'Phase II: Investigations during tunnel excavation' and 'Phase III: Investigations in the underground facilities', over a period of around 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations was planned. At the begining of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project' (hereinafter referred to as 'Observational Construction Program') and an investigation report titled 'Measurement Plan and Observational Construction Program on Drift Excavation at the Horonobe Underground Research Laboratory Project' were published. The Observational Construction Program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft, the East Shaft and the drifts in 2010 fiscal year based on the Observational Construction Program. The report summarizes the measurements data for the purpose of acquisition the basic data for

  3. Collection of URL measurement data in 2011 fiscal year at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Inagaki, Daisuke; Tokiwa, Tetsuya; Murakami, Hiroaki

    2013-02-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The URL project consists of two major research areas, 'Geoscientific Research' and 'R and D on Geological Disposal Technologies', and proceeds in three overlapping phases, 'Phase I: Surface-based investigations', 'Phase II: Investigations during tunnel excavation' and 'Phase III: Investigations in the underground facilities', over a period of around 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations was planned. At the beginning of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project' (hereinafter referred to as 'Observational Construction Program') and an investigation report titled 'Measurement Plan and Observational Construction Program on Drift Excavation at the Horonobe Underground Research Laboratory Project' were published. The Observational Construction Program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft, the East Shaft and the drifts in 2011 fiscal year based on the Observational Construction Program. The report summarizes the measurements data for the purpose of acquisition the basic data for

  4. Collection of measurement data in 2012 fiscal year at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Inagaki, Daisuke

    2014-03-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The URL project consists of two major research areas, 'Geoscientific Research' and 'R and D on Geological Disposal Technologies', and proceeds in three overlapping phases, 'Phase I: Surface-based investigations', 'Phase II: Investigations during tunnel excavation' and 'Phase III: Investigations in the underground facilities', over a period of around 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations was planned. At the begining of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project' (hereinafter referred to as 'Observational Construction Program') and an investigation report titled 'Measurement Plan and Observational Construction Program on Drift Excavation at the Horonobe Underground Research Laboratory Project' were published. The Observational Construction Program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft, the East Shaft and the drifts in 2012 fiscal year based on the Observational Construction Program. The report summarizes the measurements data for the purpose of acquisition of the basic data

  5. Cell Migration Analysis: A Low-Cost Laboratory Experiment for Cell and Developmental Biology Courses Using Keratocytes from Fish Scales

    Science.gov (United States)

    Prieto, Daniel; Aparicio, Gonzalo; Sotelo-Silveira, Jose R.

    2017-01-01

    Cell and developmental processes are complex, and profoundly dependent on spatial relationships that change over time. Innovative educational or teaching strategies are always needed to foster deep comprehension of these processes and their dynamic features. However, laboratory exercises in cell and developmental biology at the undergraduate level…

  6. Development of a Semester-Long, Inquiry-Based Laboratory Course in Upper-Level Biochemistry and Molecular Biology

    Science.gov (United States)

    Murthy, Pushpalatha P. N.; Thompson, Martin; Hungwe, Kedmon

    2014-01-01

    A semester-long laboratory course was designed and implemented to familiarize students with modern biochemistry and molecular biology techniques. The designed format involved active student participation, evaluation of data, and critical thinking, and guided students to become independent researchers. The first part of the course focused on…

  7. 94-1 Research and development project lead laboratory support. Status report, January 1--March 31, 1996

    International Nuclear Information System (INIS)

    Dinehart, M.

    1996-09-01

    This document reports status and technical progress for Los Alamos National Laboratories 94-1 Research and Development projects. An introduction to the project structure and an executive summary are included. Projects described include Electrolytic Decontamination, Combustibles, Detox, Sand, Slag, and Crucible, Surveillance, and Core Technology

  8. [New materia medica project: synthetic biology based bioactive metabolites research in medicinal plant].

    Science.gov (United States)

    Wang, Yong

    2017-03-25

    In the last decade, synthetic biology research has been gradually transited from monocellular parts or devices toward more complex multicellular systems. The emerging plant synthetic biology is regarded as the "next chapter" of synthetic biology. The complex and diverse plant metabolism as the entry point, plant synthetic biology research not only helps us understand how real life is working, but also facilitates us to learn how to design and construct more complex artificial life. Bioactive compounds innovation and large-scale production are expected to be breakthrough with the redesigned plant metabolism as well. In this review, we discuss the research progress in plant synthetic biology and propose the new materia medica project to lift the level of traditional Chinese herbal medicine research.

  9. Preliminary draft: environmental impact statement for Hot Engineering Test Project at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Boyle, J.W.; Baxter, B.J.; Carpenter, J.A.

    1978-08-01

    The project considered is the Hot Engineering Test Project (HETP), which is to be located in largely existing facilities at Oak Ridge National Laboratory (ORNL). The project is a part of the National High Temperature Gas-Cooled Reactor Fuel Recycle Program, which seeks to demonstrate the technological feasibility of the recycle processes. The HETP will attempt to confirm the operability of the processes (proven feasible in cold or nonradioactive, benchtop experimentation) under the more realistic radioactive condition. As such, the operation will involve the reprocessing and refabrication of spent HTGR fuel rods obtained from the Fort St. Vrain reactor. The reference fuel is highly enriched uranium. No significant radiological impacts are expected from routine operation of the facility to any biota or ecosystem. Concentrations of one or more radionuclides in Whiteoak Lake will increase as a result of the combination of HETP wastes with other ORNL wastes. Nonradiological effects from construction activities and routine operation should be insignificant on land and water use and on terrestrial and aquatic ecosystems. No significant socioeconomic impacts should occur from either construction or operation of the facility. Some conservative accident scenarios depict significant releases of radioactivity. Effects should be localized and would not be severe for all but the most unlikely of such incidents. No significant long-term commitment of resources is expected to be required for the project. Nor are any large quantities of scarce or critical resources likely to be irreversibly or irretrievably committed to the project. Principal alternatives considered were: relocation of the project site, postponement of the project schedule, project cancellation, and chemical process variations

  10. Engaging Students in Authentic Microbiology Research in an Introductory Biology Laboratory Course is Correlated with Gains in Student Understanding of the Nature of Authentic Research and Critical Thinking

    Directory of Open Access Journals (Sweden)

    Brittany J. Gasper

    2013-02-01

    Full Text Available Recent recommendations for biology education highlight the role of authentic research experiences early in undergraduate education as a means of increasing the number and quality of biology majors. These experiences will inform students on the nature of science, increase their confidence in doing science, as well as foster critical thinking skills, an area that has been lacking despite it being one of the desired outcomes at undergraduate institutions and with future employers. With these things in mind, we have developed an introductory biology laboratory course where students design and execute an authentic microbiology research project. Students in this course are assimilated into the community of researchers by engaging in scholarly activities such as participating in inquiry, reading scientific literature, and communicating findings in written and oral formats. After three iterations of a semester-long laboratory course, we found that students who took the course showed a significant increase in their understanding of the nature of authentic research and their level of critical thinking skills.

  11. Horonobe Underground Research Laboratory project. Investigation report for the 2007 fiscal year

    International Nuclear Information System (INIS)

    Nakayama, Masashi; Sanada, Hiroyuki; Sugita, Yutaka

    2008-09-01

    The Horonobe Underground Research Laboratory Project is planned to extend over a period of 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations', 'Phase 2: Construction Phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the results of the investigations for the 2007 fiscal year (2007/2008), the 3rd year of the Phase 2 investigations. The investigations, which are composed of 'Geoscientific research' and 'R and D on the geological disposal of high-level radioactive waste (HLW)', were carried out according to 'Horonobe Underground Research Laboratory Project Investigation Program for the 2007 Fiscal Year'. The results of these investigations, along with the results which were obtained in other departments of Japan Atomic Energy Agency (JAEA), are properly offered to the implementations and the safety regulations. JAEA proceeded with the project in collaboration with experts from domestic and overseas research organisation. (author)

  12. Role of the laboratory for laser energetics in the National Ignition Facility Project

    International Nuclear Information System (INIS)

    Soures, J.M.; Loucks, S.J.; McCrory, R.L.

    1996-01-01

    The National Ignition Facility (NIF) is a 192-beam, 1.8-MJ (ultraviolet) laser facility that is currently planned to start operating in 2002. The NIF mission is to provide data critical to this Nation's science-based stockpile stewardship (SBSS) program and to advance the understanding of inertial confinement fusion and assess its potential as an energy source. The NIF project involves a collaboration among the Lawrence Livermore National Laboratory (LLNL), Los Alamos National Laboratory (LANL), Sandia National Laboratory (SNL), and the University of Rochester's Laboratory for Laser Energetics (UR/LLE). In this paper, the role of the University of Rochester in the research, development, and planning required to assure the success of the NIF will be presented. The principal roles of the UR/LLE in the NIF are (1) validation of the direct-drive approach to NIF using the OMEGA 60-beam, 40-kJ UV laser facility; (2) support of indirect-drive physics experiments using OMEGA in collaboration with LLNL and LANL; (3) development of plasma diagnostics for NIF; (4) development of beam-smoothing techniques; and (5) development of thin-film coatings for NIF and cryogenic-fuel-layer targets for eventual application to NIF. 3 refs., 6 figs

  13. Activities of Project 'Cooperation and development with Latin America and Iberian in Biological Dosimetry of Iberian Group of Radiation Protection Societies

    International Nuclear Information System (INIS)

    Nasazzi, Nora B.; Taja, Maria R.; Giorgio, Marina di; Garcia Lima, Omar; Lamadrid, Ana I.; Olivares, Pilar; Moreno, Mercedes; Prieto, Maria J.; Espinosa, Marco

    2001-01-01

    In 1996 the GRIAPRA Group (Latin American and Iberian Group of Radiation Protection Societies) was established with the participation of Argentina, Brazil, Cuba, Mexico, Peru, Portugal and Spain. In 1998 began the biennial Collaborative Working Project 'Cooperation and Development with Latin America in Biological Dosimetry', partially supported by the Extremadura Government, Spain, initially involving five countries: Argentina, Cuba, Peru, Portugal and Spain. The general aim of the project is to create an Latin American and Iberian Biological Dosimetry Laboratories Coordinated Group in order to: give mutual cooperation and to other countries if required, in the case of radiological accident; contribute to enhance the technical capabilities of the participant laboratories; promote the installment of laboratories on this field in countries that does not have it yet through the training of human resources and providing the necessary equipment and, finally, perform jointly research activities in biological dosimetry. The activities designed in order to accomplish the project specific aims for the 1998-2000 period have been achieved. Description and results are presented. (author)

  14. Special isotope separation project, Idaho National Engineering Laboratory, Idaho Falls, Idaho

    International Nuclear Information System (INIS)

    1988-02-01

    Construction and operation of a Special Isotope Separation (SIS) project using the Atomic Vapor Laser Isotope Separation (AVLIS) process technology at the Idaho National Engineering Laboratory (INEL) near Idaho Falls, Idaho are proposed. The SIS project would process fuel-grade plutonium administered by the Department of Energy (DOE) into weapon-grade plutonium using AVLIS and supporting chemical processes. The SIS project would require construction and operation of a Laser Support Facility to house the laser system and a Plutonium Processing Facility. The SIS project would be integrated with existing support and waste management facilities at the selected site. The SIS project would provide DOE with the capability of segregating the isotopes of DOE-owned plutonium into specific isotopic concentrations. This capability would provide redundancy in production capacity, technological diversity, and flexibility in DOE's production of nuclear materials for national defense. Use of the INEL site would impact 151,350 square meters (37.4 acres) of land, of which more than 70% has been previously disturbed. During construction, plant and animal habitat associated with a sagebrush vegetation community would be lost. During operation of the SIS facilities, unavoidable radiation exposures would include occupational exposures and exposures to the public from normal atmospheric releases of radioactive materials that would be minimal compared to natural background radiation

  15. Molecular biological methods for studying the gut microbiota : the EU human gut flora project

    NARCIS (Netherlands)

    Blaut, M; Collins, MD; Welling, GW; Dore, J; van Loo, J; de Vos, W

    Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies for monitoring the gut flora in response to

  16. Hot Corrosion Test Facility at the NASA Lewis Special Projects Laboratory

    Science.gov (United States)

    Robinson, Raymond C.; Cuy, Michael D.

    1994-01-01

    The Hot Corrosion Test Facility (HCTF) at the NASA Lewis Special Projects Laboratory (SPL) is a high-velocity, pressurized burner rig currently used to evaluate the environmental durability of advanced ceramic materials such as SiC and Si3N4. The HCTF uses laboratory service air which is preheated, mixed with jet fuel, and ignited to simulate the conditions of a gas turbine engine. Air, fuel, and water systems are computer-controlled to maintain test conditions which include maximum air flows of 250 kg/hr (550 lbm/hr), pressures of 100-600 kPa (1-6 atm), and gas temperatures exceeding 1500 C (2732 F). The HCTF provides a relatively inexpensive, yet sophisticated means for researchers to study the high-temperature oxidation of advanced materials, and the injection of a salt solution provides the added capability of conducting hot corrosion studies.

  17. Two Project-Based Strategies in an Interdisciplinary Mathematical Modeling in Biology Course

    Science.gov (United States)

    Ludwig, Patrice; Tongen, Anthony; Walton, Brian

    2018-01-01

    James Madison University faculty team-teach an interdisciplinary mathematical modeling course for mathematics and biology students. We have used two different project-based approaches to emphasize the mathematical concepts taught in class, while also exposing students to new areas of mathematics not formally covered in class. The first method…

  18. Which Type of Inquiry Project Do High School Biology Students Prefer: Open or Guided?

    Science.gov (United States)

    Sadeh, Irit; Zion, Michal

    2012-01-01

    In teaching inquiry to high school students, educators differ on which method of teaching inquiry is more effective: Guided or open inquiry? This paper examines the influence of these two different inquiry learning approaches on the attitudes of Israeli high school biology students toward their inquiry project. The results showed significant…

  19. Environmental assessment of the Environmental Restoration Project at Sandia National Laboratories/New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    Sandia National Laboratories/New Mexico (SNL/NM) is managed and operated for the U.S. Department of Energy (DOE) by Sandia Corporation, a subsidiary of the Lockheed Martin Company. SNL/NM is located on land controlled by DOE within the boundaries of Kirtland Air Force Base (KAFB) in Albuquerque, New Mexico. The major responsibilities of SNL/NM are the support of national security and energy projects. This report provides an environmental assessment of proposed remedial action activities at the solid waste management units at SNL/NM. A risk assessment of health hazards is also discussed.

  20. Evaluation of an LED Retrofit Project at Princeton University's Carl Icahn Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Robert [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Murphy, Arthur [Princeton Univ., NJ (United States); Perrin, Tess [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-11-01

    At Princeton University’s Carl Icahn Laboratory, DOE’s Commercial Buildings Integration Program documented the implementation of LED retrofit products for recessed troffers, linear cove lighting, and downlights – as part of Princeton’s first building-wide interior LED project. The conversion to LED enables more extensive use of lighting controls to tailor the lighting to the task and limit the operating hours based on occupancy, and the estimated energy savings including controls is 62% compared to the incumbent system.

  1. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1993 Quality Program status report

    International Nuclear Information System (INIS)

    Boliver, S.L.

    1995-05-01

    This status report is for calendar year 1993. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, we establish a baseline that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify long term trends and to evaluate improvements. This is the third annual status report (Bolivar, 1992; Bolivar, 1994). This report is divided into two primary sections: Program Activities and Trend Analysis. Under Program Activities, programmatic issues occurring in 1993 are discussed. The goals for 1993 are also listed, followed by a discussion of their status. Lastly, goals for 1994 are identified. The Trend Analysis section is a summary of 1993 quarterly trend reports and provides a good overview of the quality assurance issues of the Los Alamos YMP

  2. Mizunami Underground Research Laboratory Project. Annual report for fiscal year 2015

    International Nuclear Information System (INIS)

    Hama, Katsuhiro; Iwatsuki, Teruki; Matsui, Hiroya; Mikake, Shinichiro; Ishibashi, Masayuki; Onoe, Hironori; Takeuchi, Ryuji; Nohara, Tsuyoshi; Sasao, Eiji; Ikeda, Koki; Koide, Kaoru

    2016-12-01

    The Mizunami Underground Research Laboratory (MIU) project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of geological disposal technologies through investigations of the deep geological environment in the crystalline host rock (granite) at Mizunami City in Gifu Prefecture, central Japan. On the occasion of the research program and management system revision of the entire JAEA organization in 2014, JAEA identified three important issues on the geoscientific research program: 'Development of countermeasure technologies for reducing groundwater inflow', 'Development of modeling technologies for mass transport' and 'Development of drift backfilling technologies', based on the latest results of the synthesizing research and development (R and D). The R and D on three important issues has been carrying out on the MIU project. In this report, the current status of R and D activities and construction in 2015 is summarized. (author)

  3. Waste management of Line Item projects at Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Zill, D.S.

    1993-01-01

    With the growing number of companies involved with today's Line Item projects at the Oak Ridge National Laboratory (ORNL), there are ever increasing problems in the handling of Radioactive Solid Low-Level Waste (SLLW). The most important of these problems is who is going to do what with the waste and when are they going to do it. The who brings to mind training; the what, compliance; and the when, cost. At ORNL, the authors have found that the best way to address the challenges of waste handling where several contractors are involved is through communication, compromise and consistency. Without these elements, opportunities bred from waste handling are likely to bring the project to a halt

  4. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1993 Quality Program status report

    Energy Technology Data Exchange (ETDEWEB)

    Bolivar, S.L.

    1995-05-01

    This status report is for calendar year 1993. It summarizes the annual activities and accomplishments of the Los Alamos National Laboratory (Los Alamos) Yucca Mountain Site Characterization Project (YMP or Project) quality assurance program. By identifying the accomplishments of the quality program, we establish a baseline that will assist in decision making, improve administrative controls and predictability, and allow us to annually identify long term trends and to evaluate improvements. This is the third annual status report (Bolivar, 1992; Bolivar, 1994). This report is divided into two primary sections: Program Activities and Trend Analysis. Under Program Activities, programmatic issues occurring in 1993 are discussed. The goals for 1993 are also listed, followed by a discussion of their status. Lastly, goals for 1994 are identified. The Trend Analysis section is a summary of 1993 quarterly trend reports and provides a good overview of the quality assurance issues of the Los Alamos YMP.

  5. Cumulative biological impacts framework for solar energy projects in the California Desert

    Science.gov (United States)

    Davis, Frank W.; Kreitler, Jason R.; Soong, Oliver; Stoms, David M.; Dashiell, Stephanie; Hannah, Lee; Wilkinson, Whitney; Dingman, John

    2013-01-01

    This project developed analytical approaches, tools and geospatial data to support conservation planning for renewable energy development in the California deserts. Research focused on geographical analysis to avoid, minimize and mitigate the cumulative biological effects of utility-scale solar energy development. A hierarchical logic model was created to map the compatibility of new solar energy projects with current biological conservation values. The research indicated that the extent of compatible areas is much greater than the estimated land area required to achieve 2040 greenhouse gas reduction goals. Species distribution models were produced for 65 animal and plant species that were of potential conservation significance to the Desert Renewable Energy Conservation Plan process. These models mapped historical and projected future habitat suitability using 270 meter resolution climate grids. The results were integrated into analytical frameworks to locate potential sites for offsetting project impacts and evaluating the cumulative effects of multiple solar energy projects. Examples applying these frameworks in the Western Mojave Desert ecoregion show the potential of these publicly-available tools to assist regional planning efforts. Results also highlight the necessity to explicitly consider projected land use change and climate change when prioritizing areas for conservation and mitigation offsets. Project data, software and model results are all available online.

  6. Reengineering of Analytical Data Management for the Environmental Restoration Project at Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Bolivar, S.; Dorries, A.; Nasser, K.; Scherma, S.

    2003-01-01

    The Environmental Restoration (ER) Project at Los Alamos National Laboratory (LANL) is responsible for the characterization, clean up, and monitoring of over 2,124 identified potential release sites (PRS). These PRSs have resulted from operations associated with weapons and energy related research which has been conducted at LANL since 1942. To accomplish mission goals, the ER Project conducts field sampling to determine possible types and levels of chemical contamination as well as their geographic extent. Last fiscal year, approximately 4000 samples were collected during ER Project field sampling campaigns. In the past, activities associated with field sampling such as sample campaign planning, paperwork, shipping and analytical laboratory tracking; verification and order fulfillment; validation and data quality assurance were performed by multiple groups working with a variety of software applications, databases and hard copy reports. This resulted in significant management and communication difficulties, data delivery delays, and inconsistent processes; it also represented a potential threat to overall data integrity. Creation of an organization, software applications and a data process that could provide for cost-effective management of the activities and data mentioned above became a management priority, resulting in a development of a reengineering task. This reengineering effort--currently nearing completion--has resulted in personnel reorganization, the development of a centralized data repository, and a powerful web-based sample management system that allows for an appreciably streamlined and more efficient data process. These changes have collectively cut data delivery times, allowed for larger volumes of samples and data to be handled with fewer personnel, and resulted in significant cost savings. This paper will provide a case study of the reengineering effort undertaken by the ER Project of its analytical data management process. It includes

  7. Reengineering of Analytical Data Management for the Environmental Restoration Project at Los Alamos National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Bolivar, S.; Dorries, A.; Nasser, K.; Scherma, S.

    2003-02-27

    The Environmental Restoration (ER) Project at Los Alamos National Laboratory (LANL) is responsible for the characterization, clean up, and monitoring of over 2,124 identified potential release sites (PRS). These PRSs have resulted from operations associated with weapons and energy related research which has been conducted at LANL since 1942. To accomplish mission goals, the ER Project conducts field sampling to determine possible types and levels of chemical contamination as well as their geographic extent. Last fiscal year, approximately 4000 samples were collected during ER Project field sampling campaigns. In the past, activities associated with field sampling such as sample campaign planning, paperwork, shipping and analytical laboratory tracking; verification and order fulfillment; validation and data quality assurance were performed by multiple groups working with a variety of software applications, databases and hard copy reports. This resulted in significant management and communication difficulties, data delivery delays, and inconsistent processes; it also represented a potential threat to overall data integrity. Creation of an organization, software applications and a data process that could provide for cost-effective management of the activities and data mentioned above became a management priority, resulting in a development of a reengineering task. This reengineering effort--currently nearing completion--has resulted in personnel reorganization, the development of a centralized data repository, and a powerful web-based sample management system that allows for an appreciably streamlined and more efficient data process. These changes have collectively cut data delivery times, allowed for larger volumes of samples and data to be handled with fewer personnel, and resulted in significant cost savings. This paper will provide a case study of the reengineering effort undertaken by the ER Project of its analytical data management process. It includes

  8. Quality Indicators in Laboratory Medicine: from theory to practice. Preliminary data from the IFCC Working Group Project "Laboratory Errors and Patient Safety".

    Science.gov (United States)

    Sciacovelli, Laura; O'Kane, Maurice; Skaik, Younis Abdelwahab; Caciagli, Patrizio; Pellegrini, Cristina; Da Rin, Giorgio; Ivanov, Agnes; Ghys, Timothy; Plebani, Mario

    2011-05-01

    The adoption of Quality Indicators (QIs) has prompted the development of tools to measure and evaluate the quality and effectiveness of laboratory testing, first in the hospital setting and subsequently in ambulatory and other care settings. While Laboratory Medicine has an important role in the delivery of high-quality care, no consensus exists as yet on the use of QIs focussing on all steps of the laboratory total testing process (TTP), and further research in this area is required. In order to reduce errors in laboratory testing, the IFCC Working Group on "Laboratory Errors and Patient Safety" (WG-LEPS) developed a series of Quality Indicators, specifically designed for clinical laboratories. In the first phase of the project, specific QIs for key processes of the TTP were identified, including all the pre-, intra- and post-analytic steps. The overall aim of the project is to create a common reporting system for clinical laboratories based on standardized data collection, and to define state-of-the-art and Quality Specifications (QSs) for each QI independent of: a) the size of organization and type of activities; b) the complexity of processes undertaken; and c) different degree of knowledge and ability of the staff. The aim of the present paper is to report the results collected from participating laboratories from February 2008 to December 2009 and to identify preliminary QSs. The results demonstrate that a Model of Quality Indicators managed as an External Quality Assurance Program can serve as a tool to monitor and control the pre-, intra- and post-analytical activities. It might also allow clinical laboratories to identify risks that lead to errors resulting in patient harm: identification and design of practices that eliminate medical errors; the sharing of information and education of clinical and laboratory teams on practices that reduce or prevent errors; the monitoring and evaluation of improvement activities.

  9. Laboratory and field studies related to the Hydrology/Radionuclide Migration Project

    International Nuclear Information System (INIS)

    Thompson, J.L.

    1991-05-01

    This annual report describes research conducted in FY 1990 by Los Alamos National Laboratory for the Hydrology/Radionuclide Migration Project. This multi-agency project measures the underground movement of radionuclides related to nuclear testing at the Nevada Test Site. This project continues the long-term experiment at the site of the Cambric nuclear test. Water pumped from a well adjacent to the explosion cavity continues to show decreasing amounts of tritium and Krypton 85 but no Cesium 139. Analyses of drillback debris shows a distinction between refractory and volatile materials in respect to both their location in the test cavity and their leachability with groundwater. We surveyed materials used during nuclear testing to evaluate any post-test hazard; we concluded that most such materials pose a minimal hazard. The Los Alamos drilling program provided an opportunity for us to sample a collapsed zone above the cavity of a test, which was fired 2 years ago. We continue our research in colloid characterization and in detection of low levels of Technetium 99 in Nevada Test Site water. During FY 1990, we drilled a new hole in the Yucca Flat area to study radionuclide migration. This report also describes Los Alamos management and planning activities in support of this project. 20 refs., 2 figs., 14 tabs

  10. Implementing the Science Assessment Standards: Developing and validating a set of laboratory assessment tasks in high school biology

    Science.gov (United States)

    Saha, Gouranga Chandra

    Very often a number of factors, especially time, space and money, deter many science educators from using inquiry-based, hands-on, laboratory practical tasks as alternative assessment instruments in science. A shortage of valid inquiry-based laboratory tasks for high school biology has been cited. Driven by this need, this study addressed the following three research questions: (1) How can laboratory-based performance tasks be designed and developed that are doable by students for whom they are designed/written? (2) Do student responses to the laboratory-based performance tasks validly represent at least some of the intended process skills that new biology learning goals want students to acquire? (3) Are the laboratory-based performance tasks psychometrically consistent as individual tasks and as a set? To answer these questions, three tasks were used from the six biology tasks initially designed and developed by an iterative process of trial testing. Analyses of data from 224 students showed that performance-based laboratory tasks that are doable by all students require careful and iterative process of development. Although the students demonstrated more skill in performing than planning and reasoning, their performances at the item level were very poor for some items. Possible reasons for the poor performances have been discussed and suggestions on how to remediate the deficiencies have been made. Empirical evidences for validity and reliability of the instrument have been presented both from the classical and the modern validity criteria point of view. Limitations of the study have been identified. Finally implications of the study and directions for further research have been discussed.

  11. Prepare, Do, Review: A skills-based approach for laboratory practical classes in biochemistry and molecular biology.

    Science.gov (United States)

    Arthur, Peter; Ludwig, Martha; Castelli, Joane; Kirkwood, Paul; Attwood, Paul

    2016-05-06

    A new laboratory practical system is described which is comprised of a number of laboratory practical modules, each based around a particular technique or set of techniques, related to the theory part of the course but not designed to be dependent on it. Each module comprises an online recorded pre-lab lecture, the laboratory practical itself and a post-lab session in which students make oral presentations on different aspects of the practical. Each part of the module is assessed with the aim of providing rapid feedback to staff and students. Each laboratory practical is the responsibility of a single staff member and through this "ownership," continual review and updating is promoted. Examples of changes made by staff to modules as a result of student feedback are detailed. A survey of students who had experienced both the old-style laboratory course and the new one provided evidence of increased satisfaction with the new program. The assessment of acquired shills in the new program showed that it was much more effective than the old course. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:276-287, 2016. © 2016 The International Union of Biochemistry and Molecular Biology.

  12. Data of fractures based on the deep borehole investigations in the Horonobe Underground Research Laboratory project. Phase 1

    International Nuclear Information System (INIS)

    Kusano, Tomohiro; Ishii, Eiichi

    2016-02-01

    Japan Atomic Energy Agency (JAEA) is performing the Horonobe Underground Research Laboratory Project, which includes a scientific study of the deep geological environment as a basis of research and development for geological disposal of high level radioactive wastes (HLW), in order to establish comprehensive techniques for the investigation, analysis and assessment of the deep geological environment in the sedimentary rock. This report aims at compiling fracture data of drill core obtained from the Horonobe Underground Research Laboratory Project (Phase 1). (author)

  13. Timely integration of safeguards and security with projects at Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Price, R.; Blount, P.M.; Garcia, S.W.; Gonzales, R.L.; Salazar, J.B.; Campbell, C.H.

    2004-01-01

    The Safeguards and Security (S and S) Requirements Integration Team at Los Alamos National Laboratory (LANL) has developed and implemented an innovative management process that will be described in detail. This process systematically integrates S and S planning into construction, facility modifications or upgrades, mission changes, and operational projects. It extends and expands the opportunities provided by the DOE project management manual, DOE M 413.3-1. Through a series of LANL documents, a process is defined and implemented that formally identifies an S and S professional to oversee, coordinate, facilitate, and communicate among the identified S and S organizations and the project organizations over the life cycle of the project. The derived benefits, namely (1) elimination/reduction of re-work or costly retrofitting, (2) overall project cost savings because of timely and improved planning, (3) formal documentation, and (4) support of Integrated Safeguards and Security Management at LANL, will be discussed. How many times, during the construction of a new facility or the modification of an existing facility, have the persons responsible for the project waited until the last possible minute or until after construction is completed to approach the security organizations for their help in safeguarding and securing the facility? It's almost like, 'Oh, by the way, do we need access control and a fence around this building and just what are we going to do with our classified anyway?' Not only is it usually difficult; it's also typically expensive to retrofit or plan for safeguards and security after the fact. Safeguards and security organizations are often blamed for budget overruns and delays in facility occupancy and program startup, but these problems are usually due to poor front-end planning. In an effort to help projects engage safeguards and security in the pre-conceptual or conceptual stages, we implemented a high level formality of operations. We

  14. PlasmaDNA: a free, cross-platform plasmid manipulation program for molecular biology laboratories

    Directory of Open Access Journals (Sweden)

    Rainy Jeffrey

    2007-09-01

    Full Text Available Abstract Background Most molecular biology experiments, and the techniques associated with this field of study, involve a great deal of engineering in the form of molecular cloning. Like all forms of engineering, perfect information about the starting material is crucial for successful completion of design and strategies. Results We have generated a program that allows complete in silico simulation of the cloning experiment. Starting with a primary DNA sequence, PlasmaDNA looks for restriction sites, open reading frames, primer annealing sequences, and various common domains. The databases are easily expandable by the user to fit his most common cloning needs. PlasmaDNA can manage and graphically represent multiple sequences at the same time, and keeps in memory the overhangs at the end of the sequences if any. This means that it is possible to virtually digest fragments, to add the digestion products to the project, and to ligate together fragments with compatible ends to generate the new sequences. Polymerase Chain Reaction (PCR fragments can also be virtually generated using the primer database, automatically adding to the fragments any 5' extra sequences present in the primers. Conclusion PlasmaDNA is a program available both on Windows and Apple operating systems, designed to facilitate molecular cloning experiments by building a visual map of the DNA. It then allows the complete planning and simulation of the cloning experiment. It also automatically updates the new sequences generated in the process, which is an important help in practice. The capacity to maintain multiple sequences in the same file can also be used to archive the various steps and strategies involved in the cloning of each construct. The program is freely available for download without charge or restriction.

  15. Los Alamos National Laboratory transuranic waste quality assurance project plan. Revision 1

    International Nuclear Information System (INIS)

    1997-01-01

    This Transuranic (TRU) Waste Quality Assurance Project Plan (QAPjP) serves as the quality management plan for the characterization of transuranic waste in preparation for certification and transportation. The Transuranic Waste Characterization/Certification Program (TWCP) consists of personnel who sample and analyze waste, validate and report data; and provide project management, quality assurance, audit and assessment, and records management support, all in accordance with established requirements for disposal of TRU waste at the Waste Isolation Pilot Plant (WIPP) facility. This QAPjP addresses how the TWCP meets the quality requirements of the Carlsbad Area Office (CAO) Quality Assurance Program Description (QAPD) and the technical requirements of the Transuranic Waste Characterization Quality Assurance Program Plan (QAPP). The TWCP characterizes and certifies retrievably stored and newly generated TRU waste using the waste selection, testing, sampling, and analytical techniques and data quality objectives (DQOs) described in the QAPP, the Los Alamos National Laboratory Transuranic Waste Certification Plan (Certification Plan), and the CST Waste Management Facilities Waste Acceptance Criteria and Certification [Los Alamos National Laboratory (LANL) Waste Acceptance Criteria (WAC)]. At the present, the TWCP does not address remote-handled (RH) waste

  16. Development of a Laboratory Project to Determine Human ABO Genotypes--Limitations Lead to Further Student Explorations

    Science.gov (United States)

    Salerno, Theresa A.

    2009-01-01

    A multiplex allele-specific PCR analysis was developed to identify six "common" genotypes: AA, AO, BB, BO, OO, and AB. This project included a pre-laboratory exercise that provided active learning experiences and developed critical thinking skills. This laboratory resulted in many successful analyses, which were verified by student knowledge of…

  17. Project-Based Learning in Undergraduate Environmental Chemistry Laboratory: Using EPA Methods to Guide Student Method Development for Pesticide Quantitation

    Science.gov (United States)

    Davis, Eric J.; Pauls, Steve; Dick, Jonathan

    2017-01-01

    Presented is a project-based learning (PBL) laboratory approach for an upper-division environmental chemistry or quantitative analysis course. In this work, a combined laboratory class of 11 environmental chemistry students developed a method based on published EPA methods for the extraction of dichlorodiphenyltrichloroethane (DDT) and its…

  18. Collection of measurement data in 2013 fiscal year at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Aoyagi, Kazuhei; Kawate, Satoshi

    2015-12-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The URL project consists of two major research areas, “Geoscientific Research” and “R and D on Geological Disposal Technologies”, and proceeds in three overlapping phases, “Phase I: Surface-based investigations”, “Phase II: Investigations during tunnel excavation” and “Phase III: Investigations in the underground facilities”, over a period of around 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations were planned. At the beginning of the Phase II investigations, an investigation report titled “Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project” (hereinafter referred to as “Observational Construction Programs”) and an investigation report titled “Measurement Plan and Observational Construction Program on Drift Excavation at the Horonobe Underground Research Laboratory Project” were published. The Observational Construction Program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft, the West Shaft and the drifts in 2013 fiscal year for the purpose of the basic data for carrying out the Observational Construction Program. A DVD-ROM is

  19. NASA's In-Space Manufacturing Project: A Roadmap for a Multimaterial Fabrication Laboratory in Space

    Science.gov (United States)

    Prater, Tracie; Werkheiser, Niki; Ledbetter, Frank

    2017-01-01

    Human space exploration to date has been limited to low Earth orbit and the moon. The International Space Station (ISS) provides a unique opportunity for NASA to partner with private industry for development and demonstration of the technologies needed to support exploration initiatives. One challenge that is critical to sustainable and safer exploration is the ability to manufacture and recycle materials in space. This paper provides an overview of NASA's in-space manufacturing (ISM) project, its past and current activities (2014-2017), and how technologies under development will ultimately culminate in a multimaterial fabrication laboratory ("ISM FabLab") to be deployed on the International Space Station in the early 2020s. ISM is a critical capability for the long endurance missions NASA seeks to undertake in the coming decades. An unanticipated failure that can be adapted for in low earth orbit, through a resupply launch or a return to earth, may instead result in a loss of mission while in transit to Mars. To have a suite of functional ISM capabilities that are compatible with NASA's exploration timeline, ISM must be equipped with the resources necessary to develop these technologies and deploy them for testing prior to the scheduled de-orbit of ISS in 2024. The presentation provides a broad overview of ISM projects activities culminating with the Fabrication Laboratory for ISS. In 2017, the in-space manufacturing project issued a broad agency announcement for this capability. Requirements of the Fabrication Laboratory as stated in the solicitation will be discussed. The FabLab will move NASA and private industry significantly closer to changing historical paradigms for human spaceflight where all materials used in space are launched from earth. While the current ISM FabLab will be tested on ISS, future systems are eventually intended for use in a deep space habitat or transit vehicle. The work of commercial companies funded under NASA's Small Business

  20. A Hybrid Integrated Laboratory and Inquiry-Based Research Experience: Replacing Traditional Laboratory Instruction with a Sustainable Student-Led Research Project

    Science.gov (United States)

    Hartings, Matthew R.; Fox, Douglas M.; Miller, Abigail E.; Muratore, Kathryn E.

    2015-01-01

    The Department of Chemistry at American University has replaced its junior- and senior-level laboratory curriculum with two, two-semester long, student-led research projects as part of the department's American Chemical Society-accredited program. In the first semester of each sequence, a faculty instructor leads the students through a set of…

  1. Improving of Quality Control and Quality Assurance in 14C and 3H Laboratory; Participation in the IAEA Model Project

    International Nuclear Information System (INIS)

    Obelic, B.

    2001-01-01

    Full text: Users of laboratory's analytical results are increasingly requiring demonstrable proofs of the reliability and credibility of the results using internationally accepted standards, because the economic, ecological, medical and legal decisions based on laboratory results need to be accepted nationally and internationally. Credibility, respect and opportunities of the laboratories are improved when objective evidence on the reliability and quality of the results can be given. This is achieved through inculcation of a quality culture through definition of well-defined procedures and controls and operational checks characteristic of quality assurance and quality control (Q A/QC). IAEA launched in 1999 a two-and-a-half year model project entitled Quality Control and Quality Assurance of Nuclear Analytical Techniques with participation of laboratories using alpha, beta and/or gamma spectrometry from CEE and NIS countries. The project started to introduce and implement QA principles in accordance with the ISO-17025 guide, leading eventually to a level at which the QA system is self-sustainable and might be appropriate for formal accreditation or certification by respective national authorities. Activities within the project consist of semi-annual reports, two training workshops, two inspection visits of the laboratories by IAEA experts and proficiency tests. The following topics were considered: organisation requirements, acceptance criteria and non-conformance management of QC, internal and external method validation, statistical analyses and uncertainty evaluation, standard operation procedures and quality manual documentation. 14 C and 3 H Laboratory of the Rudjer Boskovic Institute has been one of ten laboratories participating in the Project. In the Laboratory all the procedures required in the quality control were included implicitly, while during the Model Project much effort has been devoted to elaboration of explicit documentation. Since the beginning

  2. Collection of URL measurement data in 2007 at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Yamasaki, Masanao; Funaki, Hironori; Niinuma, Hiroaki; Fujikawa, Daisuke; Sanada, Hiroyuki; Hiraga, Naoto; Tsusaka, Kimikazu; Yamaguchi, Takehiro

    2008-11-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The project consists of two major research areas, Geoscientific Research' and 'R and D on Geological Disposal', and proceeds in three overlapping phases, 'Phase I: Surface-based investigation', 'Phase II: Construction' and 'Phase III: Operation', over a period of 20 years. The Phase I geoscientific research was planned from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations was planned. At the beginning of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project' (hereinafter referred to as 'Observational Construction Program') was published. The Observational Construction Program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft, the East Shaft and the drifts in 2007 based on the Observational Construction Program. The report summarizes for the purpose of the following: sharing the investigation and measurements data, preventing the loss of them and acquisition the basic data for carrying out the Observational Construction Program. Two DVD-ROMs are attached as an appendix. (J.P.N.)

  3. Collection of URL measurement data in 2006 at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Kumagai, Yasuhito; Funaki, Hironori; Yamasaki, Masanao; Yamaguchi, Takehiro; Sanada, Hiroyuki; Abe, Hironobu; Orukawa, Go

    2008-07-01

    The Horonobe Underground Research Laboratory (URL) Project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The project consists two major research area, Geoscientific Research' and 'R and D on Geological Disposal', and proceeds in three overlapping phases, 'Phase I: Surface-based investigation', 'Phase II: Construction' and 'Phase III: Operation', over a period of 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2005 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations was carried out. At the inception of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe Underground Research Laboratory Project' (hereinafter referred to as Observational Construction Program') was published. The Observational Construction Program summarizes followings lessons learnt from the Phase I investigations: measurements for safety/reasonable construction, measurements for R and D on enhancement of shaft design/construction technology, and measurements for verification of the deep geological environment estimated before shaft excavation. This report summarizes the measurements data acquired at the Ventilation Shaft (to approx. 50m depth) and the East Shaft (to approx. 40m depth) in 2006 based on the Observational Construction Program. CD-ROM and DVD-ROM are attached as an appendix. (J.P.N.)

  4. Results and Recommendations from the First NATO International Training Exercise on Laboratory Identification of Biological Agents

    National Research Council Canada - National Science Library

    Hancock, J.R

    2001-01-01

    ...)-inactivated biological material and one blank containing phosphate buffered saline (PBS). The United States, as the host nation, distributed PBS, Bacillus anthracis, Coxiella burnetii, Venezuelan Equine Encephalitis (VEE...

  5. Virtual Laboratories in Science Education: Students' Motivation and Experiences in Two Tertiary Biology Courses

    Science.gov (United States)

    Dyrberg, Nadia Rahbek; Treusch, Alexander H.; Wiegand, Claudia

    2017-01-01

    Potential benefits of simulations and virtual laboratory exercises in natural sciences have been both theorised and studied recently. This study reports findings from a pilot study on student attitude, motivation and self-efficacy when using the virtual laboratory programme Labster. The programme allows interactive learning about the workflows and…

  6. The Protein Information Management System (PiMS): a generic tool for any structural biology research laboratory

    International Nuclear Information System (INIS)

    Morris, Chris; Pajon, Anne; Griffiths, Susanne L.; Daniel, Ed; Savitsky, Marc; Lin, Bill; Diprose, Jonathan M.; Wilter da Silva, Alan; Pilicheva, Katya; Troshin, Peter; Niekerk, Johannes van; Isaacs, Neil; Naismith, James; Nave, Colin; Blake, Richard; Wilson, Keith S.; Stuart, David I.; Henrick, Kim; Esnouf, Robert M.

    2011-01-01

    The Protein Information Management System (PiMS) is described together with a discussion of how its features make it well suited to laboratories of all sizes. The techniques used in protein production and structural biology have been developing rapidly, but techniques for recording the laboratory information produced have not kept pace. One approach is the development of laboratory information-management systems (LIMS), which typically use a relational database schema to model and store results from a laboratory workflow. The underlying philosophy and implementation of the Protein Information Management System (PiMS), a LIMS development specifically targeted at the flexible and unpredictable workflows of protein-production research laboratories of all scales, is described. PiMS is a web-based Java application that uses either Postgres or Oracle as the underlying relational database-management system. PiMS is available under a free licence to all academic laboratories either for local installation or for use as a managed service

  7. The Protein Information Management System (PiMS): a generic tool for any structural biology research laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Morris, Chris [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Pajon, Anne [Wellcome Trust Genome Campus, Hinxton CB10 1SD (United Kingdom); Griffiths, Susanne L. [University of York, Heslington, York YO10 5DD (United Kingdom); Daniel, Ed [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Savitsky, Marc [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Lin, Bill [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Diprose, Jonathan M. [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Wilter da Silva, Alan [Wellcome Trust Genome Campus, Hinxton CB10 1SD (United Kingdom); Pilicheva, Katya [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Troshin, Peter [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Niekerk, Johannes van [University of Dundee, Dundee DD1 5EH, Scotland (United Kingdom); Isaacs, Neil [University of Glasgow, Glasgow G12 8QQ, Scotland (United Kingdom); Naismith, James [University of St Andrews, St Andrews, Fife KY16 9ST, Scotland (United Kingdom); Nave, Colin; Blake, Richard [STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom); Wilson, Keith S. [University of York, Heslington, York YO10 5DD (United Kingdom); Stuart, David I. [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); Henrick, Kim [Wellcome Trust Genome Campus, Hinxton CB10 1SD (United Kingdom); Esnouf, Robert M., E-mail: robert@strubi.ox.ac.uk [University of Oxford, Roosevelt Drive, Oxford OX3 7BN (United Kingdom); STFC Daresbury Laboratory, Warrington WA4 4AD (United Kingdom)

    2011-04-01

    The Protein Information Management System (PiMS) is described together with a discussion of how its features make it well suited to laboratories of all sizes. The techniques used in protein production and structural biology have been developing rapidly, but techniques for recording the laboratory information produced have not kept pace. One approach is the development of laboratory information-management systems (LIMS), which typically use a relational database schema to model and store results from a laboratory workflow. The underlying philosophy and implementation of the Protein Information Management System (PiMS), a LIMS development specifically targeted at the flexible and unpredictable workflows of protein-production research laboratories of all scales, is described. PiMS is a web-based Java application that uses either Postgres or Oracle as the underlying relational database-management system. PiMS is available under a free licence to all academic laboratories either for local installation or for use as a managed service.

  8. Impact of Cooperative Learning Approaches on Students’ Academic Achievement and Laboratory Proficiency in Biology Subject in Selected Rural Schools, Ethiopia

    Directory of Open Access Journals (Sweden)

    Eyayu Molla

    2018-01-01

    Full Text Available The main objective of this study was to evaluate the impact of cooperative learning methods on students’ academic achievement and laboratory proficiency in biology subject. Quasi-experimental control group interrupted time series design was employed. Data pertaining to these variables were collected from 369 students and 18 biology teachers in three schools. A series of biological tests and semistructured questionnaire were used to collect data. Multivariate analysis (two-way ANOVA was used to analyze the test scores exposed by teaching methods, and semistructured questionnaire was administered to comprehend factors that hamper the successive execution of CL. Hence, multivariate analysis revealed that there was no significant (P>0.05 difference in the pretest score of the learner academic performance; however, there were significant differences (P<0.01 in the posttest results by teaching methods, but not by schools. Correspondingly, there were significant differences in the pretest P<0.05 and posttest (P<0.01 results of the students’ laboratory proficiency by teaching methods. The results exemplify that there was significant learning gain obtained via CLAD followed by cooperative discussion group (CDG. The result from the questionnaire survey showed that the number of students, lack of laboratory equipment, and so on hamper consecutive execution of CL.

  9. Dicentric chromosome aberration analysis using giemsa and centromere specific fluorescence in-situ hybridization for biological dosimetry: An inter- and intra-laboratory comparison in Indian laboratories

    International Nuclear Information System (INIS)

    Bhavani, M.; Tamizh Selvan, G.; Kaur, Harpreet; Adhikari, J.S.; Vijayalakshmi, J.; Venkatachalam, P.; Chaudhury, N.K.

    2014-01-01

    To facilitate efficient handling of large samples, an attempt towards networking of laboratories in India for biological dosimetry was carried out. Human peripheral blood samples were exposed to 60 Co γ-radiation for ten different doses (0–5 Gy) at a dose rate of 0.7 and 2 Gy/min. The chromosomal aberrations (CA) were scored in Giemsa-stained and fluorescence in-situ hybridization with centromere-specific probes. No significant difference (p>0.05) was observed in the CA yield for given doses except 4 and 5 Gy, between the laboratories, among the scorers and also staining methods adapted suggest the reliability and validates the inter-lab comparisons exercise for triage applications. - Highlights: • This is the first report from India on Networking for Biological Dosimetry preparedness using dicentric chromosomal (DC) aberration assay. • There is no significant difference in the in vitro dose response curve (Slope, Intercept, Curvature) constructed among the two labs. • No significant variation in the scoring of DC aberrations between the scorers irrespective of labs. • The DC results obtained by the labs from the Giemsa stained metaphase preparations were confirmed with centromere specific-FISH for further reliability and validity

  10. Using a Module-Based Laboratory to Incorporate Inquiry into a Large Cell Biology Course

    Science.gov (United States)

    Howard, David R.; Miskowski, Jennifer A.

    2005-01-01

    Because cell biology has rapidly increased in breadth and depth, instructors are challenged not only to provide undergraduate science students with a strong, up-to-date foundation of knowledge, but also to engage them in the scientific process. To these ends, revision of the Cell Biology Lab course at the University of Wisconsin-La Crosse was…

  11. Restoration projects for decontamination of facilities from chemical, biological and radiological contamination after terrorist actions

    Energy Technology Data Exchange (ETDEWEB)

    Fingas, M.; Volchek, K.; Lumley, T.; Thouin, G.; Harrison, S.; Kuang, W. [Environment Canada, Ottawa, ON (Canada). Emergencies Science and Technology Division, Environmental Technology Centre, Science and Technology Branch; Payette, P.; Laframboise, D.; Best, M. [Public Health Agency of Canada, Ottawa, ON (Canada); Krishnan, J.; Wagener, S.; Bernard, K.; Majcher, M. [Public Health Agency of Canada, Winnipeg, MB (Canada); Cousins, T.; Jones, T. [Defence Research and Development Canada, Ottawa, ON (Canada); Velicogna, D.; Hornof, M.; Punt, M. [SAIC Canada, Ottawa, ON (Canada)

    2006-07-01

    This paper reviewed studies that identified better decontamination methods for chemical, biological and radiological/nuclear (CBRN) attacks. In particular, it reviewed aspects of 3 projects in which procedures were tested and validated for site restoration. Cleanup targets or standards for decontaminating buildings and materials after a CBRN attack were also developed. The projects were based on physicochemical and toxicological knowledge of potential terrorist agents and selected surface matrices. The projects also involved modeling and assessing environmental and health risks. The first multi-agent project involved gathering information on known procedures for restoration of areas including interiors and exteriors of buildings, contents, parking lots, lawn, and vehicles. Air inside the building was included. The efficacy of some of the proposed concepts was tested. Results included the determination of appropriate surrogates for anthrax and tests of liquid and gaseous biocides on the surrogates. The development of new contamination procedures using peroxyacetic acid were also discussed. The second project involved decontamination tests on CBRN using specially-constructed buildings at the Counter-terrorism Technology Centre at Defence Research and Development Canada in Suffield. The buildings will be contaminated with chemical and biological agents and with short-lived radionuclides. They will be decontaminated using the best-performing technologies known. Information collected will include fate of the contaminant and decontamination products, effectiveness of the restoration methods, cost and duration of cleanup and logistical problems. The third project is aimed at developing cleanup standards for decontaminating buildings and construction materials after a chemical or biological attack. It will create as many as 12 algorithms for the development of 50 standards which will help cleanup personnel and first-responders to gauge whether proposed methods can achieve

  12. Cell migration analysis: A low-cost laboratory experiment for cell and developmental biology courses using keratocytes from fish scales.

    Science.gov (United States)

    Prieto, Daniel; Aparicio, Gonzalo; Sotelo-Silveira, Jose R

    2017-11-01

    Cell and developmental processes are complex, and profoundly dependent on spatial relationships that change over time. Innovative educational or teaching strategies are always needed to foster deep comprehension of these processes and their dynamic features. However, laboratory exercises in cell and developmental biology at the undergraduate level do not often take into account the time dimension. In this article, we provide a laboratory exercise focused in cell migration, aiming to stimulate thinking in time and space dimensions through a simplification of more complex processes occurring in cell or developmental biology. The use of open-source tools for the analysis, as well as the whole package of raw results (available at http://github.com/danielprieto/keratocyte) make it suitable for its implementation in courses with very diverse budgets. Aiming to facilitate the student's transition from science-students to science-practitioners we propose an exercise of scientific thinking, and an evaluation method. This in turn is communicated here to facilitate the finding of common caveats and weaknesses in the process of producing simple scientific communications describing the results achieved. © 2017 by The International Union of Biochemistry and Molecular Biology, 45(6):475-482, 2017. © 2017 The International Union of Biochemistry and Molecular Biology.

  13. 94-1 Research and development project lead laboratory support. Status report, January 1--March 31, 1997

    Energy Technology Data Exchange (ETDEWEB)

    Rink, N.A. [comp.

    1997-08-01

    This status report is published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials that resulted from weapons production throughout the DOE complex. This report summarizes status and technical progress for Los Alamos 94-1 projects during the second quarter of fiscal year 1997.

  14. 94-1 Research and development project lead laboratory support. Status report, January 1--March 31, 1997

    International Nuclear Information System (INIS)

    Rink, N.A.

    1997-08-01

    This status report is published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials that resulted from weapons production throughout the DOE complex. This report summarizes status and technical progress for Los Alamos 94-1 projects during the second quarter of fiscal year 1997

  15. SAIL Panama Canal Zone Project 2008 : Biological Survey of Panama (1910-1912)

    OpenAIRE

    DeHart, Liz; Haas, Stephanie C.; Walton, Jennifer; Heil, Kathy

    2009-01-01

    During the 18th Annual 2008 SAIL meeting at the Smithsonian Tropical Research Institute in Panama, a suggestion was made for the need to digitize and make available through the Aquatic Commons some of the early documents related to the U.S. biological survey of Panama from 1910 to 1912. With SAIL’s endeavor, a new digital project was born and this presentation describes its process, beginning to final product. The main source consulted for determining copyright clear publications was: ...

  16. Influence of biological and ecological factors on the radio-sensitivity of laboratory animals

    International Nuclear Information System (INIS)

    Guenet, J.L.; Legeay, G.

    1968-01-01

    The biological and ecological factors liable to induce a change in the radio-sensitivity of a species are undoubtedly responsible for the large fluctuations observed during radio-biological experiments. It is easy to limit or to suppress the effects of some of them (genetic or nutritional factors). Since the research worker cannot control the others it is necessary to take them into account. In this report the authors analyse the action of two factors chosen as examples: - the first concerns biological rhythms; - the second attempts to define the role of health conditions. Other factors will be dealt with in a later report. (authors) [fr

  17. ISS National Laboratory Education Project: Enhancing and Innovating the ISS as an Educational Venue

    Science.gov (United States)

    Melvin, Leland D.

    2011-01-01

    The vision is to develop the ISS National Laboratory Education Project (ISS NLE) as a national resource for Science, Technology, Engineering and Mathematics (STEM) education, utilizing the unique educational venue of the International Space Station per the NASA Congressional Authorization Act of 2005. The ISS NLE will serve as an educational resource which enables educational activities onboard the ISS and in the classroom. The ISS NLE will be accessible to educators and students from kindergarten to post-doctoral studies, at primary and secondary schools, colleges and universities. Additionally, the ISS NLE will provide ISS-related STEM education opportunities and resources for learners of all ages via informal educational institutions and venues Though U.S. Congressional direction emphasized the involvement of U.S. students, many ISS-based educational activities have international student and educator participation Over 31 million students around the world have participated in several ISS-related education activities.

  18. Chemval project report on stage 2: application of speciation models to laboratory and field data sets

    International Nuclear Information System (INIS)

    Read, D.

    1990-01-01

    This report describes the results of Chemval Stage 2, an international chemical model validation exercise involving the active participation of 14 organizations within EC countries, Sweden, Switzerland and Finland. Four different sites have been studied, representing a range of host rock types and proposed disposal facilities. It details the methodology employed and results obtained for 19 test cases of varying complexity, devised to allow comparison between modelling results and experimental measurements. Recommendations are made for enhancement of models and thermodynamic databases employed, as well as for the collection of field/laboratory data geared to the needs of model validation. This report complements a previous one, ref. EUR 12237 EN, devoted to the verification of speciation models. The work is part of the Community's Mirage project - Second phase, on migration of radionuclides in the geosphere

  19. Final report for the Integrated and Robust Security Infrastructure (IRSI) laboratory directed research and development project

    Energy Technology Data Exchange (ETDEWEB)

    Hutchinson, R.L.; Hamilton, V.A.; Istrail, G.G.; Espinoza, J.; Murphy, M.D.

    1997-11-01

    This report describes the results of a Sandia-funded laboratory-directed research and development project titled {open_quotes}Integrated and Robust Security Infrastructure{close_quotes} (IRSI). IRSI was to provide a broad range of commercial-grade security services to any software application. IRSI has two primary goals: application transparency and manageable public key infrastructure. IRSI must provide its security services to any application without the need to modify the application to invoke the security services. Public key mechanisms are well suited for a network with many end users and systems. There are many issues that make it difficult to deploy and manage a public key infrastructure. IRSI addressed some of these issues to create a more manageable public key infrastructure.

  20. Laboratory projects using inquiry-based learning: an application to a practical inorganic course

    Directory of Open Access Journals (Sweden)

    José G. Carriazo

    2011-01-01

    Full Text Available This paper reports how laboratory projects (LP coupled to inquiry-based learning (IBL were implemented in a practical inorganic chemistry course. Several coordination compounds have been successfully synthesised by students according to the proposed topics by the LP-IBL junction, and the chemistry of a number of metals has been studied. Qualitative data were collected from written reports, oral presentations, lab-notebook reviews and personal discussions with the students through an experimental course with undergraduate second-year students at the Universidad Nacional de Colombia during the last 5 years. Positive skills production was observed by combining LP and IBL. Conceptual, practical, interpretational, constructional (questions, explanations, hypotheses, communicational, environmental and application abilities were revealed by the students throughout the experimental course.

  1. Biological degradation of EDTA in pulping effluents at higher pH - a laboratory study

    Energy Technology Data Exchange (ETDEWEB)

    Ek, M; Remberger, M; Allard, A S

    1999-01-01

    The biological degradation of EDTA at different pH, sludge load and sludge age has been investigated in laboratory experiments. The experiments showed that relatively fast degradation of EDTA in the form found in this waste water (from production of TMP) took place at least at pH around 8.5 with moderate COD load and high sludge age. In continuous reactors the degradation of EDTA in a pulp and paper waste water was 2-3 mg EDTA/g SS*day at both pH 7 and 8,5, and at sludge ages from 5 to 21 days. The degradation was dependent on sludge load, and no degradation was seen above 1 g COD/g SS*day. In kinetic experiments with half strength waste water the same degradation rate (1,5-2 mg EDTA/g SS*day) was found at pH 7 and at pH 8,5 with sludge of low age (9 and 5 days SRT). Much faster degradation was found at pH 8,5 with sludge of high age (21 days in the continuous experiment). The mean degradation rate was over 10 mg EDTA/g SS*day from 20 to 5 mg EDTA/l. v{sub max} was determined to be 35 mg EDTA/g SS*day and K{sub M} to 31 mg EDTA/l. COD removal was at least as good at pH 8,5 as at pH 7. Sludge properties were best at pH 8,5 and long sludge retention time (giving low sludge load). Both sludge volume index and residual suspended solids after sedimentation were lower than under normal conditions at pH 7. The direct cost for caustic lime would be about 15 SEK per ton of TMP, with a water like the one investigated here. This can vary a lot depending on starting pH and buffering capacity. Costs for addition of nitrogen source could probably be omitted, but this is normally not more than 1-2 SEK per ton of TMP. The extra need for oxygen in the treatment would not be more than some percent, but may be important if oxygen is limited. A substantial extra cost would be if the aeration volume has to be increased. According to the best results from the kinetic study, this would not be needed in an extended aeration activated plant with 2 days HRT and sludge concentrations of 2

  2. Quality improvement project in cervical cancer screening: practical measures for monitoring laboratory performance.

    Science.gov (United States)

    Tarkkanen, Jussi; Geagea, Antoine; Nieminen, Pekka; Anttila, Ahti

    2003-01-01

    We conducted a quality improvement project in a cervical cancer screening programme in Helsinki in order to see if detection of precancerous lesions could be influenced by external (participation rate) and internal (laboratory praxis) quality measures. In order to increase the participation rate, a second personal invitation to Pap-test was mailed to nonparticipants of the first call. In order to improve the quality of screening, the cytotechnicians monitored their performance longitudinally by recording the number of slides reviewed per day, the pick-up rate of abnormal smears, the report of the consulting cytopathologist, and the number of histologically verified lesions detected from the cases that they had screened. Regular sessions were held to compare the histological findings with the cytological findings of all cases referred for colposcopy. No pressure was applied on the cytotechnicians to ensure that they felt comfortable with their daily workload. A total of 110 000 smears were screened for cervical cancer at the Helsinki City Hospital during 1996-99. Initially, the overall participation rate increased from 62% to 71%. The number of histologically confirmed precancerous lesions (CIN 1-3) more than doubled and their detection rate increased from 0.32% to 0.72%. Continuous education and feedback from daily work performance were important, yet rather inexpensive means in increasing laboratory performance. Additional measures are needed to further increase the participation rate. Impact of the quality measures on cancer incidence needs to be assessed later on.

  3. Towards European urinalysis guidelines. Introduction of a project under European Confederation of Laboratory Medicine.

    Science.gov (United States)

    Kouri, T T; Gant, V A; Fogazzi, G B; Hofmann, W; Hallander, H O; Guder, W G

    2000-07-01

    Improved standardized performance is needed because urinalysis continues to be one of the most frequently requested laboratory tests. Since 1997, the European Confederation of Laboratory Medicine (ECLM) has been supporting an interdisciplinary project aiming to produce European urinalysis guidelines. More than seventy clinical chemists, microbiologists and ward-based clinicians, as well as representatives of manufacturers are taking part. These guidelines aim to improve the quality and consistency of chemical urinalysis, particle counting and bacterial culture by suggesting optimal investigative processes that could be applied in Europe. The approach is based on medical needs for urinalysis. The importance of the pre-analytical stage for total quality is stressed by detailed illustrative advice for specimen collection. Attention is also given to emerging automated technology. For cost containment reasons, both optimum (ideal) procedures and minimum analytical approaches are suggested. Since urinalysis mostly lacks genuine reference methods (primary reference measurement procedures; Level 4), a novel classification of the methods is proposed: comparison measurement procedures (Level 3), quantitative routine procedures (Level 2), and ordinal scale examinations (Level 1). Stepwise strategies are suggested to save costs, applying different rules for general and specific patient populations. New analytical quality specifications have been created. After a consultation period, the final written text will be published in full as a separate document.

  4. Final report on the surface-based investigation (phase 1) at the Mizunami Underground Laboratory project

    International Nuclear Information System (INIS)

    Saegusa, Hiromitsu; Seno, Yasuhiro; Nakama, Shigeo; Tsuruta, Tadahiko; Amano, Kenji; Takeuchi, Ryuji; Matsuoka, Toshiyuki; Onoe, Hironori; Mizuno, Takashi; Ohyama, Takuya; Hama, Katsuhiro; Sato, Toshinori; Kuji, Masayoshi; Kuroda, Hidetaka; Semba, Takeshi; Uchida, Masahiro; Sugihara, Kozo; Sakamaki, Masanori; Iwatsuki, Teruki

    2007-03-01

    The Mizunami Underground Laboratory (MIU) Project is a comprehensive research project investigating the deep underground environment within crystalline rock being conducted by Japan Atomic Energy Agency at Mizunami City in Gifu Prefecture, central Japan and its role is defined in 'Framework for Nuclear Energy Policy' by Japan Atomic Energy Commission. The MIU Project has three overlapping phases: Surface-based Investigation phase (Phase I), Construction phase (Phase II), and Operation phase (Phase III), with a total duration of 20 years. The overall project goals of the MIU Project from Phase I through to Phase III are: 1) to establish techniques for investigation, analysis and assessment of the deep geological environment, and 2) to develop a range of engineering for deep underground application. During Phase I, the overall project goals were supported by Phase I goals. For the overall project goals 1), the Phase I goals were set to construct models of the geological environment from all surface-based investigation results that describe the geological environment prior to excavation and predict excavation response. For the overall project goals 2), the Phase I goals were set to formulate detailed design concepts and a construction plan for the underground facilities. This report summarizes the Phase I investigation which was completed in March 2005. The authors believe this report will make an important milestone, since this report clarifies how the Phase I goals are achieved and evaluate the future issues thereby direct the research which will be conducted during Phase II. With regard to the overall project goals 1), 'To establish techniques for investigation, analysis and assessment of the deep geological environment,' a step-wise investigation was conducted by iterating investigation, interpretation, and assessment, thereby understanding of geologic environment was progressively and effectively improved with progress of investigation. An optimal procedure from

  5. Objectives of research activities in Biology Branch, Chalk River Nuclear Laboratories, 1976

    International Nuclear Information System (INIS)

    1977-03-01

    The primary responsibility assigned to the Biology Branch within the framework of CRNL has been an active engagement in basic research related to the assessment of radiation hazards, particularly those to be expected after exposure to relatively low doses of radiation delivered at low dose-rates. The present group is characterized by a broad interest in the entire chain of events by which the initial radiation-induced changes in the living cell are translated into biological effects, with a special focus of attention on the mechanisms by which the initial damage can be largely repaired and by which the risks to man are modified under different circumstances. The basic concepts in radiation biology and risk estimates are reviewed in the light of recent literature on these topics. The current and proposed research activities of the Biology Branch are described. General and specific recommendations for future activities are given. (author)

  6. Horonobe Underground Research Laboratory Project. Plans for surface-based investigations. Phase 1

    International Nuclear Information System (INIS)

    Goto, Junichi; Hama, Katsuhiro

    2003-10-01

    The Horonobe Underground Research Laboratory Project is an investigation project which is planned over 20 years. The investigations are conducted in the three phases: investigations from surface (Phase 1), investigations during construction of the underground facility (Phase 2) and investigations using the facility (Phase 3). Taking into account the results from 'H12: Project of Establish the Scientific and Technical Basis for HLW Disposal in Japan - Second Progress Report on Research and Development for the Geological Disposal of HLW in Japan-' (JNC, 2000), research and development goals for the Horonobe URL project were re-defined as follows; a) Development of investigation technologies for the geological environment, b) Development of monitoring technologies for the geological environment, c) Study on the long-term stability of the geological environment, d) Development of the basis for engineering technologies in deep underground, e) Verification of technologies for engineered barriers, f) Development of detailed designing technologies of the repositories, and g) Improvement of safety assessment methodologies. Investigations for the goals a) to d) and e) to g) are conducted in the 'Geoscientific Research' and 'Research and Development on Geological Disposal', respectively. In Phase 1, a 'laboratory construction area' of a few kilometers square is selected based on the results from early stage investigations. Subsequent investigations are concentrated in the selected area and its periphery. Acquisition of data by surface-based investigations, modeling of the geological environment and predictions of changes in the geological environment caused by the construction of the underground facility, are conducted in a) Development of investigation technologies for the geological environment. Development and installation of monitoring equipments and data acquisition prior to the construction of the underground facility fall under b) Development of monitoring technologies

  7. The environmental and molecular sciences laboratory project: Continuous evolution in leadership

    International Nuclear Information System (INIS)

    Knutson, D.E.; McClusky, J.K.

    1995-09-01

    The United States is embarking on an environmental cleanup effort that dwarfs previous scientific enterprise. Using current best available technology, the projected costs of cleaning up the tens of abounds of toxic waste sites, including DOE sites, is estimated to exceed one trillion dollars. That level of expenditure contains no guarantee that the sites can be restored to their original condition, and no consensus on ''how clean is clean enough.'' ''Ultimately, the scientific challenge is to determine as accurately as possible each term in the path that links the source of the contaminant with the particular biological end points or health effects and to understand the mechanisms that connect them. However, the present state of scientific knowledge regarding the effects of exogenous chemicals on human biology is very limited. Understanding the connections at the molecular level is, at best, a blurred picture and often a black box.'' Long term environmental research at the molecular level is needed to resolve the concerns, and form the building blocks for a structure of cost effective process improvement and regulatory reform

  8. The environmental and molecular sciences laboratory project: Continuous evolution in leadership

    Energy Technology Data Exchange (ETDEWEB)

    Knutson, D.E.; McClusky, J.K.

    1995-09-01

    The United States is embarking on an environmental cleanup effort that dwarfs previous scientific enterprise. Using current best available technology, the projected costs of cleaning up the tens of abounds of toxic waste sites, including DOE sites, is estimated to exceed one trillion dollars. That level of expenditure contains no guarantee that the sites can be restored to their original condition, and no consensus on ``how clean is clean enough.`` ``Ultimately, the scientific challenge is to determine as accurately as possible each term in the path that links the source of the contaminant with the particular biological end points or health effects and to understand the mechanisms that connect them. However, the present state of scientific knowledge regarding the effects of exogenous chemicals on human biology is very limited. Understanding the connections at the molecular level is, at best, a blurred picture and often a black box.`` Long term environmental research at the molecular level is needed to resolve the concerns, and form the building blocks for a structure of cost effective process improvement and regulatory reform.

  9. Status report of the ESR/alanine project of the IAEA Dosimetry Laboratory

    International Nuclear Information System (INIS)

    Girzikowsky, R.

    1990-01-01

    The main tasks of the Dosimetry Laboratory of the IAEA are in the field of therapy-level dosimetry. Other dose ranges, i.e. protection-level standardization and calibration, are partly covered, too. On high-dose level the laboratory has been offering a Fricke-dosimetry service to Member States on request since 1965. In 1985 the Dosimetry Section has initiated an International Dose Assurance Service (IDAS). This service is an important part of the Agency's high-dose standardization programme and is based on Electron-Spin-Resonance (ESR) analysis of radiation-induced free radicals in alanine. This ESR/alanine dosimetry system was initially developed for high-dose application by GSF/Munich as the Agency's outside contractor. Although efforts were undertaken to equip the Agency Laboratory with an ESR analyzer since 1984, the purchase of an adequate unit was postponed until the end of 1988. From the date of establishment of the IDAS programme until today, the handling of all technical aspects, i.e. dosimeter production and evaluation was and is carried out by GSP/Munich under IAEA contract. As mentioned above, the IAEA Dosimetry Laboratory is in possession of its own ESR analyzer since December 1988. It was then installed in May 1989. Only one staff member of the laboratory was assigned to this project. He made himself acquainted with the analyzer, the measuring technique, and the application of alanine as dosimeter material. The ESR spectrometric analyzer is a CW ESR type ESP 300 manufactured by Bruker GmbH/FRG. It consists of a 9''/2,7 kW magnet, an X-band microwave bridge, a field regulator unit, a signal channel unit and a data system ESP 1620 based on the 68020 CPU. The probe material selected for measurement of concentration of radiation-induced free radicals is L-Alanine. This amino acid is a suitable material for transfer dosimeters due to its properties, i.e. wide sensitivity range (10 Gy to 100 kGy), energy independence for high-energy photons and electron

  10. Project Management Plan for the Idaho National Engineering Laboratory Waste Isolation Pilot Plant Experimental Test Program

    International Nuclear Information System (INIS)

    Connolly, M.J.; Sayer, D.L.

    1993-11-01

    EG ampersand G Idaho, Inc. and Argonne National Laboratory-West (ANL-W) are participating in the Idaho National Engineering Laboratory's (INEL's) Waste Isolation Pilot Plant (WIPP) Experimental Test Program (WETP). The purpose of the INEL WET is to provide chemical, physical, and radiochemical data on transuranic (TRU) waste to be stored at WIPP. The waste characterization data collected will be used to support the WIPP Performance Assessment (PA), development of the disposal No-Migration Variance Petition (NMVP), and to support the WIPP disposal decision. The PA is an analysis required by the Code of Federal Regulations (CFR), Title 40, Part 191 (40 CFR 191), which identifies the processes and events that may affect the disposal system (WIPP) and examines the effects of those processes and events on the performance of WIPP. A NMVP is required for the WIPP by 40 CFR 268 in order to dispose of land disposal restriction (LDR) mixed TRU waste in WIPP. It is anticipated that the detailed Resource Conservation and Recovery Act (RCRA) waste characterization data of all INEL retrievably-stored TRU waste to be stored in WIPP will be required for the NMVP. Waste characterization requirements for PA and RCRA may not necessarily be identical. Waste characterization requirements for the PA will be defined by Sandia National Laboratories. The requirements for RCRA are defined in 40 CFR 268, WIPP RCRA Part B Application Waste Analysis Plan (WAP), and WIPP Waste Characterization Program Plan (WWCP). This Project Management Plan (PMP) addresses only the characterization of the contact handled (CH) TRU waste at the INEL. This document will address all work in which EG ampersand G Idaho is responsible concerning the INEL WETP. Even though EG ampersand G Idaho has no responsibility for the work that ANL-W is performing, EG ampersand G Idaho will keep a current status and provide a project coordination effort with ANL-W to ensure that the INEL, as a whole, is effectively and

  11. Building an internet-based workflow system - the case of Lawrence Livermore National Laboratories` Zephyr project

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, C. W., LLNL

    1998-04-01

    Lawrence Livermore National Laboratories` Zephyr System provides a showcase for the ways in which emerging technologies can help streamline procurement processes and improve the coordination between participants in engineering projects by allowing collaboration in ways that have not been possible before. The project also shows the success of a highly pragmatic approach that was initiated by the end user community, and that intentionally covered standard situations, rather than aiming at also automating the exceptions. By helping push purchasing responsibilities down to the end user, thereby greatly reducing the involvement of the purchasing department in operational activities, it was possible to streamline the process significantly resulting in time savings of up to 90%, major cost reductions, and improved quality. Left with less day-to- day purchasing operations, the purchasing department has more time for strategic tasks such as selecting and pre-qualifying new suppliers, negotiating blanket orders, or implementing new procurement systems. The case shows once more that the use of information technologies can result in major benefits when aligned with organizational adjustments.

  12. Educational radio to encourage the scientific vocations: The ‘Laboratory Mice’ project

    Directory of Open Access Journals (Sweden)

    Daniel Martín-Pena

    2018-05-01

    Full Text Available The aim of the scientific dissemination projectLaboratory mice’ is to approach to science, through education radio, to elementary students in Extremadura (Spain. This innovative initiative has allowed to reach nearly half a thousand students between 9 and 13 years old. An informative experience with a novel work methodology consisting in the elaboration of radio spaces where the leads have been the students themselves, who have previously received practical workshops carried out by researchers from the University of Extremadura and who explain the scientific issues of daily life. In this article is hoped to explain all the stages of Project and investigation instruments that have been used to know the student experiences and how have actions influenced in the public perception of science between the school community at an early ages. The main conclusion has been the improvement of the perception by the student of the work done by the scientists and the increase of the interest in the subjects connected to the scientific scope in favor of the STEM vocations.

  13. Which Type of Inquiry Project Do High School Biology Students Prefer: Open or Guided?

    Science.gov (United States)

    Sadeh, Irit; Zion, Michal

    2012-10-01

    In teaching inquiry to high school students, educators differ on which method of teaching inquiry is more effective: Guided or open inquiry? This paper examines the influence of these two different inquiry learning approaches on the attitudes of Israeli high school biology students toward their inquiry project. The results showed significant differences between the two groups: Open inquiry students were more satisfied and felt they gained benefits from implementing the project to a greater extent than guided inquiry students. On the other hand, regarding documentation throughout the project, guided inquiry students believed that they conducted more documentation, as compared to their open inquiry peers. No significant differences were found regarding `the investment of time', but significant differences were found in the time invested and difficulties which arose concerning the different stages of the inquiry process: Open inquiry students believed they spent more time in the first stages of the project, while guided inquiry students believed they spent more time in writing the final paper. In addition, other differences were found: Open inquiry students felt more involved in their project, and felt a greater sense of cooperation with others, in comparison to guided inquiry students. These findings may help teachers who hesitate to teach open inquiry to implement this method of inquiry; or at least provide their students with the opportunity to be more involved in inquiry projects, and ultimately provide their students with more autonomy, high-order thinking, and a deeper understanding in performing science.

  14. Horonobe Underground Research Laboratory project. Investigation program for the 2008 fiscal year

    International Nuclear Information System (INIS)

    Nakayama, Masashi; Sanada, Hiroyuki; Yamaguchi, Takehiro; Sugita, Yutaka

    2008-09-01

    As part of the research and development program on geological disposal of high-level radioactive waste (HLW), the Horonobe Underground Research Center, a division of the Japan Atomic Energy Agency (JAEA), is implementing the Horonobe Underground Research Laboratory Project (Horonobe URL Project) with the aim at investigating sedimentary rock formations. According to the research plan described in the Midterm Plan of JAEA, geological investigations are to be carried out during the drilling of a shaft down to intermediate depth, while research and development in the areas of engineering technology and safety assessment are to be promoted by collaboration with other research organizations. The results of the R and D activities will be systematized as a 'knowledge base' that supports a wide range of arguments related to the safety of geological disposal. The Horonobe URL Project is planned to extend over a period of 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations', 'Phase 2: Construction phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the investigation program for the 2008 fiscal year (2008/2009), the 4th year of the Phase 2 investigations. In the 2008 fiscal year, investigations in geoscientific research', including 'development of techniques for investigating the geological environment', 'development of techniques for long-term monitoring of the geological environment', 'development of engineering techniques for use in the deep underground environment' and studies on the long-term stability of the geological environment', are continuously carried out. Investigations in 'research and development on geological disposal technology', including 'improving the reliability of disposal technologies' and 'enhancement of safety assessment methodologies', are also continuously carried out

  15. Manipulatives-Based Laboratory for Majors Biology – a Hands-On Approach to Understanding Respiration and Photosynthesis

    Directory of Open Access Journals (Sweden)

    Sarah M. Boomer

    2011-09-01

    Full Text Available The first course in our year-long introductory series for Biology majors encompasses four learning units: biological molecules and cells, metabolism, genetics, and evolution. Of these, the metabolism unit, which includes respiration and photosynthesis, has shown the lowest student exam scores, least interest, and lowest laboratory ratings. Consequently, we hypothesized that modeling metabolic processes in the laboratory would improve student content learning during this course unit. Specifically, we developed manipulatives-based laboratory exercises that combined paper cutouts, movable blocks, and large diagrams of the cell. In particular, our novel use of connecting LEGO blocks allowed students to move model electrons and phosphates between molecules and within defined spaces of the cell. We assessed student learning using both formal (content indicators and attitude surveys and informal (the identification of misconceptions or discussions with students approaches. On the metabolism unit content exam, student performance improved by 46% over pretest scores and by the end of the course, the majority of students rated metabolism as their most-improved (43% and favorite (33% subject as compared with other unit topics. The majority of students rated manipulatives-based labs as very helpful, as compared to non-manipulatives-based labs. In this report, we will demonstrate that students made learning gains across all content areas, but most notably in the unit that covered respiration and photosynthesis.

  16. Biological monitoring and abatement program plan for Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Kszos, L.A.; Anderson, G.E.; Gregory, S.M.; Peterson, M.J.; Ryon, M.G.; Schilling, E.M.; Smith, J.G.; Southworth, G.R.; Phipps, T.L.

    1997-06-01

    The overall purpose of this plan is to evaluate the receiving streams' biological communities for the duration of the permit and meet the objectives for the ORNL BMAP as outlined in the NPDES permit (Appendix). The ORNL BMAP will focus on those streams in the WOC watershed that (1) receive NPDES discharges and (2) have been identified as ecologically impacted. In response to the newly issued NPDES permit, the tasks that are included in this BMAP plan include monitoring biological communities (fish and benthic invertebrates), monitoring mercury contamination in fish and water, monitoring polychlorinated biphenyl (PCB) contamination in fish, and evaluating temperature loading from ORNL outfalls. The ORNL BMAP will evaluate the effects of sediment and oil and grease, as well as the chlorine control strategy through the use of biological community data. Monitoring will be conducted at sites in WOC, First Creek, Fifth Creek, Melton Branch, and WOL

  17. Biological monitoring and abatement program plan for Oak Ridge National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Kszos, L.A.; Anderson, G.E.; Gregory, S.M.; Peterson, M.J.; Ryon, M.G.; Schilling, E.M.; Smith, J.G.; Southworth, G.R. [Oak Ridge National Lab., TN (United States); Phipps, T.L. [CKY, Inc., Oak Ridge, TN (United States)

    1997-06-01

    The overall purpose of this plan is to evaluate the receiving streams` biological communities for the duration of the permit and meet the objectives for the ORNL BMAP as outlined in the NPDES permit (Appendix). The ORNL BMAP will focus on those streams in the WOC watershed that (1) receive NPDES discharges and (2) have been identified as ecologically impacted. In response to the newly issued NPDES permit, the tasks that are included in this BMAP plan include monitoring biological communities (fish and benthic invertebrates), monitoring mercury contamination in fish and water, monitoring polychlorinated biphenyl (PCB) contamination in fish, and evaluating temperature loading from ORNL outfalls. The ORNL BMAP will evaluate the effects of sediment and oil and grease, as well as the chlorine control strategy through the use of biological community data. Monitoring will be conducted at sites in WOC, First Creek, Fifth Creek, Melton Branch, and WOL.

  18. 94-1 Research and development project lead laboratory support. Status report, July 1, 1996--September 30, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Rink, N. [comp.

    1997-03-01

    This document reports status and technical progress for Los Alamos 94-1 Research and Development projects. Updated schedule charts are shown in the appendix. This is the fourth status report published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management (DOE/EM) funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials resulting from weapons production throughout the DOE complex. This document also serves as an end-for-year review of projects and positions the program for FY97.

  19. Error budget calculations in laboratory medicine: linking the concepts of biological variation and allowable medical errors

    NARCIS (Netherlands)

    Stroobants, A. K.; Goldschmidt, H. M. J.; Plebani, M.

    2003-01-01

    Background: Random, systematic and sporadic errors, which unfortunately are not uncommon in laboratory medicine, can have a considerable impact on the well being of patients. Although somewhat difficult to attain, our main goal should be to prevent all possible errors. A good insight on error-prone

  20. A Linked Series of Laboratory Exercises in Molecular Biology Utilizing Bioinformatics and GFP

    Science.gov (United States)

    Medin, Carey L.; Nolin, Katie L.

    2011-01-01

    Molecular biologists commonly use bioinformatics to map and analyze DNA and protein sequences and to align different DNA and protein sequences for comparison. Additionally, biologists can create and view 3D models of protein structures to further understand intramolecular interactions. The primary goal of this 10-week laboratory was to introduce…

  1. Integrating Biology into the General Chemistry Laboratory: Fluorometric Analysis of Chlorophyll "a"

    Science.gov (United States)

    Wesolowski, Meredith C.

    2014-01-01

    A laboratory experiment that introduces fluorometry of chlorophyll "a" at the general chemistry level is described. The use of thin-layer chromatography to isolate chlorophyll "a" from spirulina and leaf matter enables quantification of small amounts of chlorophyll "a" via fluorometry. Student results were reasonably…

  2. Introducing Human Population Biology through an Easy Laboratory Exercise on Mitochondrial DNA

    Science.gov (United States)

    Pardinas, Antonio F.; Dopico, Eduardo; Roca, Agustin; Garcia-Vazquez, Eva; Lopez, Belen

    2010-01-01

    This article describes an easy and cheap laboratory exercise for students to discover their own mitochondrial haplogroup. Students use buccal swabs to obtain mucosa cells as noninvasive tissue samples, extract DNA, and with a simple polymerase chain reaction-restriction fragment length polymorphism analysis they can obtain DNA fragments of…

  3. Using Biocatalysis to Integrate Organic Chemistry into a Molecular Biology Laboratory Course

    Science.gov (United States)

    Beers, Mande; Archer, Crystal; Feske, Brent D.; Mateer, Scott C.

    2012-01-01

    Current cutting-edge biomedical investigation requires that the researcher have an operational understanding of several diverse disciplines. Biocatalysis is a field of science that operates at the crossroads of organic chemistry, biochemistry, microbiology, and molecular biology, and provides an excellent model for interdisciplinary research. We…

  4. An intercomparison of γ-spectrometry on two samples of biological origin by eight laboratories in four countries

    International Nuclear Information System (INIS)

    Twining, J.R.

    1996-01-01

    This report gives details of the first inter-laboratory comparison of γ-spectrometry to be run within SPERA, the South Pacific Environmental Radioactivity Association since its inauguration in 1991. Laboratories in Australia, Chile, French Polynesia and New Zealand participated in the exercise. Two 'unknown' samples of biological origin were analysed. The first was a sample of milk powder derived from IAEA reference material. This sample provided an assessment of overall accuracy of 134 Cs, 137 Cs and 40 K determinations. The second sample consisted of dried fish flesh including natural 40 K and spiked with a mixed nuclide solution containing 210 Pb, 109 Cd, 54 Mn, 60 Co and trace 133 Ba. Together the samples gave information on analytical precision over a range of energies and activities. When the results were compared with the recommended values and confidence intervals of the IAEA reference material, the overall accuracy of the γ-spectrometry analytical procedures was found to be good. The average mean values for combined laboratory data fell within the recommended value ranges for each isotope. Ninety percent of the individual laboratory isotope mean values were within two standard errors of the 95% confidence interval of the standard, 75% were within 1 s.e., and 33% of the analyses fell within the confidence interval. The largest sources of error were derived from reporting and calculating of results which gave a 16% gross error rate. (Author)

  5. Results of single borehole hydraulic tests in the Mizunami Underground Research Laboratory project. FY 2012 - FY 2015

    International Nuclear Information System (INIS)

    Onoe, Hironori; Takeuchi, Ryuji

    2016-11-01

    This report summarize the results of the single borehole hydraulic tests of 151 sections carried out at the -300 m Stage and the -500 m Stage of the Mizunami Underground Research Laboratory from FY 2012 to FY 2015. The details of each test (test interval depth, geology, etc.) as well as the interpreted hydraulic parameters and analytical methods used are presented in this report. Furthermore, the previous results of the single borehole hydraulic tests carried out in the Regional Hydrogeological Study Project and the Mizunami Underground Research Laboratory Project before FY 2012 are also summarized in this report. (author)

  6. Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

    Energy Technology Data Exchange (ETDEWEB)

    Loar, J.M.; Adams, S.M.; Allison, L.J.; Blaylock, B.G.; Boston, H.L.; Huston, M.A.; Kimmel, B.L.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Walton, B.T.; Kitchings, J.T.; Olsen, C.R.

    1991-09-01

    On April 1, 1986, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge National Laboratory (ORNL) (EPA 1986). As specified in Part 3: Special Conditions (Item H) of the permit, a plan for biological monitoring of the Clinch River, White Oak Creek (WOC), Northwest Tributary (NWT) of WOC, Melton Branch (MB), Fifth Creek, and First Creek shall be submitted for approval to the US Environmental Protection Agency (EPA) and the Tennessee Department of Health and Environment (TDHE) within 90 days of the effective date of the permit. The plan, which is referred to in Part 3 (H) of the permit as the Biological Monitoring Plan and Abatement Program (BMPAP), describes characterization monitoring studies to be conducted for the duration of the permit (5 years). In order to be consistent with the terminology used for the Biological Monitoring and Abatement Programs for the Oak Ridge Y-12 Plan and the Oak Ridge K-25 Plant, BMPAP will subsequently be referred to as the Biological Monitoring and Abatement Program (BMAP). The proposed BMAP outlined in this document is based on preliminary discussions held on December 9, 1985, between staff of Martin Marietta Energy Systems, Inc. (ORNL and Central Management), the US Department of Energy (DOE), EPA, and TDHE. 232 refs., 11 figs., 7 tabs.

  7. Oak Ridge National Laboratory Biological Monitoring and Abatement Program for White Oak Creek Watershed and the Clinch River

    International Nuclear Information System (INIS)

    Loar, J.M.; Adams, S.M.; Allison, L.J.; Blaylock, B.G.; Boston, H.L.; Huston, M.A.; Kimmel, B.L.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Walton, B.T.; Kitchings, J.T.; Olsen, C.R.

    1991-09-01

    On April 1, 1986, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge National Laboratory (ORNL) (EPA 1986). As specified in Part 3: Special Conditions (Item H) of the permit, a plan for biological monitoring of the Clinch River, White Oak Creek (WOC), Northwest Tributary (NWT) of WOC, Melton Branch (MB), Fifth Creek, and First Creek shall be submitted for approval to the US Environmental Protection Agency (EPA) and the Tennessee Department of Health and Environment (TDHE) within 90 days of the effective date of the permit. The plan, which is referred to in Part 3 (H) of the permit as the Biological Monitoring Plan and Abatement Program (BMPAP), describes characterization monitoring studies to be conducted for the duration of the permit (5 years). In order to be consistent with the terminology used for the Biological Monitoring and Abatement Programs for the Oak Ridge Y-12 Plan and the Oak Ridge K-25 Plant, BMPAP will subsequently be referred to as the Biological Monitoring and Abatement Program (BMAP). The proposed BMAP outlined in this document is based on preliminary discussions held on December 9, 1985, between staff of Martin Marietta Energy Systems, Inc. (ORNL and Central Management), the US Department of Energy (DOE), EPA, and TDHE. 232 refs., 11 figs., 7 tabs

  8. Data Quality Objectives Summary Report for the Demolition of the Masonry Block for the 108-F Biological Laboratory

    International Nuclear Information System (INIS)

    Byrnes, M. E.

    1999-01-01

    The purpose of this data quality objectives (DQO) process is to support decision-making activities as they pertain to the demolition and disposition (or disposal) of the uncoated 108-F Biological Laboratory masonry block walls. The objective of DQO Step 1 is to use the information gathered from the DQO scoping process and other relevant information to clearly and concisely state the problem to be resolved. The free-form text sections included in this step are intended to define the project objectives and assumptions, present the project issues, summarize the facility background information, and provide a concise statement of the problem. The tables provided in this section are designed to document the personnel involved in the DQO process, identify the contaminants of concern, and summarize the key information needed to support the writing of the problem statement. The purpose of DQO Step 2 is to define the principal study question (PSQ) that needs to be resolved to address the problem identified in DQO Step 1 and to define the alternative actions that would result from the resolution of the PSQ. The PSQ and alternative actions are combined into a decision statement that expresses a choice among alternative actions. The purpose of DQO Step 3 is to identify the type of data needed to resolve the decision statement identified in DQO Step 2, as well as the analytical performance requirements (e.g., practical quantitation limit [PQL] requirement, precision, and accuracy) for the data. If it is determined that the required data do not already exist, the data may either be derived from computational or surveying/sampling and analysis methods. The primary objective of DQO Step 4 is for the DQO Team to identify the geographic (spatial) and temporal boundaries of the facility under investigation, as well as practical constraints (i.e., hindrances or obstacles) that must be taken into consideration in the surveying design. Implementing this step ensures that the surveying

  9. [Establishing biological reference intervals of alanine transaminase for clinical laboratory stored database].

    Science.gov (United States)

    Guo, Wei; Song, Binbin; Shen, Junfei; Wu, Jiong; Zhang, Chunyan; Wang, Beili; Pan, Baishen

    2015-08-25

    To establish an indirect reference interval based on the test results of alanine aminotransferase stored in a laboratory information system. All alanine aminotransferase results were included for outpatients and physical examinations that were stored in the laboratory information system of Zhongshan Hospital during 2014. The original data were transformed using a Box-Cox transformation to obtain an approximate normal distribution. Outliers were identified and omitted using the Chauvenet and Tukey methods. The indirect reference intervals were obtained by simultaneously applying nonparametric and Hoffmann methods. The reference change value was selected to determine the statistical significance of the observed differences between the calculated and published reference intervals. The indirect reference intervals for alanine aminotransferase of all groups were 12 to 41 U/L (male, outpatient), 12 to 48 U/L (male, physical examination), 9 to 32 U/L (female, outpatient), and 8 to 35 U/L (female, physical examination), respectively. The absolute differences when compared with the direct results were all smaller than the reference change value of alanine aminotransferase. The Box-Cox transformation combined with the Hoffmann and Tukey methods is a simple and reliable technique that should be promoted and used by clinical laboratories.

  10. Collection of measurement data in 2014 fiscal year at the Horonobe Underground Research Laboratory Project

    International Nuclear Information System (INIS)

    Sakurai, Akitaka; Aoyagi, Kazuhei

    2016-07-01

    The Horonobe Underground Research Laboratory (URL) Project has being pursued by Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The URL project consists of two major research areas, 'Geoscientific Research' and 'R and D on Geological Disposal Technologies', and proceeds in three overlapping phases, 'Phase I: Surface-based investigations', 'Phase II: Investigations during tunnel excavation' and 'Phase III: Investigations in the underground facilities', over a period of around 20 years. The Phase I geoscientific research was carried out from March 2001 to March 2006 in parallel with design and execution scheme on URL facilities. In addition, identifying key issues that need to be addressed in the Phase II/III investigations were planned. At the beginning of the Phase II investigations, investigation reports related to measurement plan and observational construction program on shaft and drift excavation were published. The observational construction program summarizes the followings from the results of the Phase I investigations: measurements for safety and reasonable constructions, enhancement of shaft design and construction technologies and evaluation of appropriateness for the deep geological environment model estimated before shaft excavation. Currently, Phase III investigation related to geological disposal in underground facilities has been conducting. Also, measurement for consideration of long-term stability of the tunnel has been continued. This report summarizes the measurements data acquired at the West Shaft in 2014 fiscal year for the purpose of the basic data for carrying out the Observational Construction Program. A DVD-ROM is attached as an appendix. (J.P.N.)

  11. Horonobe Underground Research Laboratory project. Synthesis of phase I investigation 2001-2005. Volume 'geoscientific research'

    International Nuclear Information System (INIS)

    Ota, Kunio; Abe, Hironobu; Kunimaru, Takanori

    2011-03-01

    The Horonobe Underground Research Laboratory (URL) Project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe in Hokkaido, northern Japan. The project consists of two major research areas, 'Geoscientific Research' and 'R and D on Geological Disposal', and proceeds in three overlapping phases, 'Phase I: Surface-based investigation', 'Phase II: Construction' and 'Phase III: Operation', over a period of 20 years. The present report summarises the results of the Phase I geoscientific research carried out from March 2001 to March 2005. Integration of the results from different disciplines ensures that the Phase I goals have been successfully achieved and identifies key issues that need to be addressed in Phases II and III. More importantly, efforts are made to summarise as many lessons learnt from the Phase I investigations and other technical achievements as possible to form a 'knowledge base' that will reinforce the technical basis for both implementation and the formulation of safety regulations. Based on experiences of selecting the URL area and site in Horonobe Town, important factors that should be taken into consideration in such selection processes and their rationale are demonstrated. In the course of stepwise surface-based investigations, a number of achievements have been made, which can eventually provide examples of integrated methodologies for characterising the sedimentary formations. The relevant surface-based investigation techniques have thus been further developed. The Horonobe URL has been designed based on geoscientific information accumulated during the surface-based investigations and the plans for safe construction and operation of the URL have been defined in a feasible manner. In addition, a variety of environmental measures taken during Phase I have proved to be

  12. Large-scale demonstration and deployment project at Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Brown, S.; McFee, J.; Broom, C.; Dugger, H.; Stallings, E.

    1999-01-01

    Established by the US Department of Energy (DOE) Environmental Management program through its Office of Science and Technology, the Deactivation and Decommissioning Focus Area is developing answers to the technological problems that hinder Environmental Management's extensive cleanup efforts. The optimized application of technologies to ongoing nuclear facility decontamination and dismantlement is critical in meeting the challenge of decommissioning approximately 9,000 buildings and structures within the DOE complex. The significant technical and economic concerns in this area underscore a national imperative for the qualification and timely delivery of cost-reduction technologies and management approaches to meet federal and private needs. At Los Alamos National Laboratory (LANL), a Large-Scale Demonstration and Deployment Project (LSDDP) has been established to facilitate demonstration and deployment of technologies for the characterization, decontamination, and volume reduction of oversized metallic waste, mostly in the form of gloveboxes contaminated with transuranic radionuclides. The LANL LSDDP is being managed by an integrated contractor team (ICT) consisting of IT Corporation, ICF Incorporated, and Florida International University and includes representation from LANL's Environmental Management Program Office. The ICT published in the Commerce Business Daily a solicitation for interest for innovative technologies capable of improving cost and performance of the baseline process. Each expression of interest response was evaluated and demonstration contract negotiations are under way for those technologies expected to be capable of meeting the project objectives. This paper discusses management organization and approach, the results of the technology search, the technology selection methodology, the results of the selection process, and future plans for the program

  13. Mizunami Underground Research Laboratory project. Rock mechanical investigations annual report for fiscal year 2013

    International Nuclear Information System (INIS)

    Sato, Toshinori; Sanada, Hiroyuki; Tanno, Takeo

    2015-02-01

    In order to establish the scientific and technical basis for geological disposal of technology, Japan Atomic Energy Agency (JAEA) is pursuing the geoscientific research project namely the Mizunami Underground Research Laboratory (MIU) in the crystalline rock environment at Tono Geoscience Center (TGC). In the MIU Project, geoscientific research is being carried out in three overlapping phases; Surface-based Investigation Phase (Phase I: FY1996 - 2004), Construction Phase (Phase II: FY2004- in progress) and Operation Phase (Phase III: FY2010- in progress). In the rock mechanical investigations at the Phase II, the research aims at “Characterization of geological environment in the Excavation Disturbed Zone (EDZ)” from the viewpoint of safety assessment. For the research, the specific information of the EDZ such as (1) size and structures, (2) petrophysical/geomechanical properties, and (3) stress state are required. The research also aims at “Characterization of geomechanical stability around tunnel” from the viewpoint of design and construction of underground facilities. For the research, the specific information such as (4) local stress regime, (5) spatial variability of petrophysical/geomechanical properties of rocks, and (6) distribution of discontinuities intersecting underground tunnels are required. The measurement system for rock mass behavior has been manufactured and set for groundwater recovery experiment in the Phase III. This report presents the results of following rock mechanical investigations conducted in FY 2013. In-situ stress measurements using Compact Conical-ended Borehole Overcoring Technique were performed at the - 500m stage. Measurement system for rock mass displacement using optical fiber was installed at the - 500m stage as part of the groundwater recovery experiment. Study on the modeling based on equivalent continuum model was continued. Phenomenological study and theoretical study on long-term behavior of crystalline rock were

  14. The quality control for biological-shield heavy concrete construction of nuclear power project

    International Nuclear Information System (INIS)

    Sun Hongjun; Ma Xinchao

    2012-01-01

    The paper introduces the function and characteristics of biological protective heavy-concrete, and its main application scope and role in Fangjiashan nuclear power project. From the aspects of raw material selection, mixing ratio test, heavy concrete production, the paper discusses the main control points of heavy concrete construction process, points out the basic characteristics of heavy concrete construction, and put forward measures to prevent density non-uniformity during heavy concrete construction and to control slump during transportation. Results prove that reasonable construction process control can assure the engineering quality. (authors)

  15. Consortium biology in immunology: the perspective from the Immunological Genome Project.

    Science.gov (United States)

    Benoist, Christophe; Lanier, Lewis; Merad, Miriam; Mathis, Diane

    2012-10-01

    Although the field has a long collaborative tradition, immunology has made less use than genetics of 'consortium biology', wherein groups of investigators together tackle large integrated questions or problems. However, immunology is naturally suited to large-scale integrative and systems-level approaches, owing to the multicellular and adaptive nature of the cells it encompasses. Here, we discuss the value and drawbacks of this organization of research, in the context of the long-running 'big science' debate, and consider the opportunities that may exist for the immunology community. We position this analysis in light of our own experience, both positive and negative, as participants of the Immunological Genome Project.

  16. Epidemiology, biology and therapy of Merkel cell carcinoma: conclusions from the EU project IMMOMEC

    DEFF Research Database (Denmark)

    Becker, Juergen C.; Stang, Andreas; zur Hausen, Axel

    2018-01-01

    Merkel cell carcinoma (MCC) is a highly aggressive, often lethal neuroendocrine cancer. Its carcinogenesis may be either caused by the clonal integration of the Merkel cell polyomavirus into the host genome or by UV-induced mutations. Notably, virally-encoded oncoproteins and UV-induced mutations...... knowledge on epidemiology, biology and therapy of MCC as conclusion of the project 'Immune Modulating strategies for treatment of Merkel Cell Carcinoma', which was funded over a 5-year period by the European Commission to investigate innovative immunotherapies for MCC....

  17. The Los Alamos National Laboratory Chemistry and Metallurgy Research Facility upgrades project - A model for waste minimization

    International Nuclear Information System (INIS)

    Burns, M.L.; Durrer, R.E.; Kennicott, M.A.

    1996-07-01

    The Los Alamos National Laboratory (LANL) Chemistry and Metallurgy Research (CMR) Facility, constructed in 1952, is currently undergoing a major, multi-year construction project. Many of the operations required under this project (i.e., design, demolition, decontamination, construction, and waste management) mimic the processes required of a large scale decontamination and decommissioning (D ampersand D) job and are identical to the requirements of any of several upgrades projects anticipated for LANL and other Department of Energy (DOE) sites. For these reasons the CMR Upgrades Project is seen as an ideal model facility - to test the application, and measure the success of - waste minimization techniques which could be brought to bear on any of the similar projects. The purpose of this paper will be to discuss the past, present, and anticipated waste minimization applications at the facility and will focus on the development and execution of the project's open-quotes Waste Minimization/Pollution Prevention Strategic Plan.close quotes

  18. Surface radiological free release program for the Battelle Columbus Laboratory Decommissioning Project

    International Nuclear Information System (INIS)

    Horton, C.N.

    1995-01-01

    This paper was prepared for the Second Residual Radioactivity and Recycling Criteria Workshop and discusses decommissioning and decontamination activities at the Battelle Columbus Laboratories Decommissioning Project (BCLDP). The BCLDP is a joint effort between the Department of Energy (DOE) and Battelle Columbus Operations to decontaminate fifteen Battelle-owned buildings contaminated with DOE radioactive materials. The privately owned buildings located across the street from The Ohio State University campus became contaminated with natural uranium and thorium during nuclear research activities. BCLDP waste management is supported by an extensive radiological free-release program. Miscellaneous materials and building surfaces have been free-released from the BCLDP. The free-release program has substantially reduced radioactive waste volumes and supported waste minimization. Free release for unrestricted use has challenged regulators and NRC licensees since the development of early surface-release criteria. This paper discusses the surface radiological free-release program incorporated by the BCLDP and the historical development of the surface radiological free-release criteria. Concerns regarding radiological free-release criteria are also presented. (author)

  19. Horonobe Underground Research Laboratory project investigation program for the 2007 fiscal year (Translated document)

    International Nuclear Information System (INIS)

    Matsui, Hiroya; Nakayama, Masashi; Sanada, Hiroyuki; Yamaguchi, Takehiro

    2008-09-01

    As past of the research and development program on the geological disposal of high-level radioactive waste (HLW), the Horonobe Underground Research Center, a division of the Japan Atomic Energy Agency (JAEA), is implementing the Horonobe Underground Research Laboratory Project (Horonobe URL Project) with the aim at investigating sedimentary rock formations. According to the research plan described in the Midterm Plan of JAEA, geological investigations are to be carried out during the drilling of a shaft down to intermediate depth, while research and development in the areas of engineering technology and safety assessment are to be promoted by collaboration with other research organizations. The results of the R and D activities will be systematized as a 'knowledge base' that supports a wide range of arguments related to the safety of geological disposal. The Horonobe URL Project is planned to extend over a period of 20 years. The investigations will be conducted in three phases, namely 'Phase 1: Surface-based investigations', 'Phase 2: Construction phase' (investigations during construction of the underground facilities) and 'Phase 3: Operation phase' (research in the underground facilities). This report summarizes the investigation program for the 2007 fiscal year (2007/2008), the third year of the Phase 2 investigations. In the 2007 fiscal year, investigations in geoscientific research', including 'development of techniques for investigating the geological environment', 'development of techniques for use in the deep underground environment' and 'studies on the long-term stability of the geological environment', is continuously carried out. Investigations in 'research and development on geological disposal technology', including improving the reliability of disposal technologies' and 'enhancement of safety assessment methodologies' are also continuously carried out. Construction of the underground facilities is ongoing at the Ventilation Shaft and the East Shaft

  20. Ensuring comparability of data generated by multiple analytical laboratories for environmental decision making at the Fernald Environmental Management Project

    International Nuclear Information System (INIS)

    Sutton, C.; Campbell, B.A.; Danahy, R.J.; Dugan, T.A.; Tomlinson, F.K.

    1994-01-01

    The Fernald Environmental Management Project is a US Department of Energy (DOE)-owned facility located 17 miles northwest of Cincinnati, Ohio. From 1952 until 1989, the Fernald site provided high-purity uranium metal products to support US defense programs. In 1989 the mission of Fernald changed from one of uranium production to one of environmental restoration. At Fernald, multiple functional programs require analytical data. Inorganic and organic data for these programs are currently generated by seven laboratories, while radiochemical data are being obtained from six laboratories. Quality Assurance (QA) and Quality Control (QC) programs have been established to help ensure comparability of data generated by multiple laboratories at different times. The quality assurance program for organic and inorganic measurements specifies which analytical methodologies and sample preparation procedures are to be used based on analyte class, sample matrix, and data quality requirements. In contrast, performance specifications have been established for radiochemical analyses. A blind performance evaluation program for all laboratories, both on-site and subcontracted commercial laboratories, provides continuous feedback on data quality. The necessity for subcontractor laboratories to participate in the performance evaluation program is a contractual requirement. Similarly, subcontract laboratories are contractually required to generate data which meet radiochemical performance specifications. The Fernald on-site laboratory must also fulfill these requirements

  1. A Semester-Long Project for Teaching Basic Techniques in Molecular Biology Such as Restriction Fragment Length Polymorphism Analysis to Undergraduate and Graduate Students

    Science.gov (United States)

    DiBartolomeis, Susan M.

    2011-01-01

    Several reports on science education suggest that students at all levels learn better if they are immersed in a project that is long term, yielding results that require analysis and interpretation. I describe a 12-wk laboratory project suitable for upper-level undergraduates and first-year graduate students, in which the students molecularly locate and map a gene from Drosophila melanogaster called dusky and one of dusky's mutant alleles. The mapping strategy uses restriction fragment length polymorphism analysis; hence, students perform most of the basic techniques of molecular biology (DNA isolation, restriction enzyme digestion and mapping, plasmid vector subcloning, agarose and polyacrylamide gel electrophoresis, DNA labeling, and Southern hybridization) toward the single goal of characterizing dusky and the mutant allele dusky73. Students work as individuals, pairs, or in groups of up to four students. Some exercises require multitasking and collaboration between groups. Finally, results from everyone in the class are required for the final analysis. Results of pre- and postquizzes and surveys indicate that student knowledge of appropriate topics and skills increased significantly, students felt more confident in the laboratory, and students found the laboratory project interesting and challenging. Former students report that the lab was useful in their careers. PMID:21364104

  2. Marketing and Distribution: What About Training Plans in the DE Project Laboratory?

    Science.gov (United States)

    Snyder, Ruth

    1977-01-01

    Managing a distributive education (DE) laboratory is a challenge. The laboratory is the simulated training station, with the instructor taking on the role of employer, managing student activities and learning. One tool to be utilized in managing a DE laboratory is a training plan. This article discusses the need for student training plans and the…

  3. The role of the molecular biology laboratory in the management of chronic hepatitis B and C

    Directory of Open Access Journals (Sweden)

    Peter Karayiannis

    2013-03-01

    Full Text Available Molecular biology techniques are routinely used nowadays to diagnose and evaluate antiviral treatment of patients with chronic hepatitis B (HBV and hepatitis C virus (HCV infections. Current tools at our disposal include tests that quantify the amount of circulating virus in the blood, techniques that can analyse genomic sequences to determine viral genotypes or subtypes, or determine amino-acid substitutions that may confer resistance to existing antiviral drugs. What is more, continuously evolving serological tests for the detection of viral antigens or their corresponding antibodies, have made diagnosis of disease as sensitive as possible. The present review will concentrate primarily on molecular diagnostics.

  4. Evaluation of laser phosphorimetry for the analysis of uranium in biological samples from laboratory animal studies

    International Nuclear Information System (INIS)

    Gray, D.; Eidsom, A.F.

    1985-01-01

    Laser phosphorimetry has been used for uranium analyses in a variety of sample matrices, including environmental and human bioassay samples. The Scientrex-UA-3 Uranium Analyzer has been used at ITRI to acquire data on the applicability of laser phosphorimetry to analyses of uranium in the highly concentrated solutions resulting from chemical processing of biological comparisons of results with those obtained from conventional fluorometry. These comparisons have been very favorable for many sample types. Results of these comparisons and an evaluation of the data obtained with the Scintrex unit are presented

  5. Project management plan for the isotopes facilities deactivation project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-08-01

    Purpose of the deactivation project is to place former isotopes production facilities at ORNL in a safe, stable, and environmentally sound condition suitable for an extended period of minimum surveillance and maintenance. This management plan was prepared to document project objectives, define organizational relationships and responsibilities, and outline the management control systems. The project has adopted the strategy of deactivating the simple facilities first. The plan provides a road map for the quality assurance program and identifies other documents supporting the Isotopes Facilities Deactivation Project

  6. Neutron structural biology

    International Nuclear Information System (INIS)

    Schoenborn, B.

    1997-01-01

    This is the final report of a one-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). We investigated design concepts of neutron scattering capabilities for structural biology at spallation sources. This included the analysis of design parameters for protein crystallography as well as membrane diffraction instruments. These instruments are designed to be general user facilities and will be used by scientists from industry, universities, and other national laboratories

  7. Data of groundwater from boreholes, river water and precipitation for the Horonobe Underground Research Laboratory project. 2011-2010

    Energy Technology Data Exchange (ETDEWEB)

    Amano, Yuki; Yamamoto, Yoichi; Nanjyo, Isao; Murakami, Hiroaki; Yokota, Hideharu; Yamazaki, Masanori; Iwatsuki, Teruki [Japan Atomic Energy Agency, Geological Isolation Research and Development Directorate, Horonobe, Hokkaido (Japan); Kunimaru, Takanori [Japan Atomic Energy Agency, Geological Isolation Research and Development Directorate, Mizunami, Gifu (Japan); Oyama, Takahiro [Central Research Inst. of Electric Power Industry, Tokyo (Japan)

    2012-02-15

    In the Horonobe Underground Research Laboratory (URL) Project, groundwater from boreholes, river water and precipitation have been analyzed for the environmental monitoring since the fiscal year 2001. This report shows the data set of water chemistry since the fiscal year 2001 to the fiscal year 2010. (author)

  8. Preparation of (+)-α-terpineol from (+)-limonene: monoterpenes with pleasant odor in a project for undergraduate organic chemistry laboratory

    International Nuclear Information System (INIS)

    Baptistella, Lucia Helena Brito; Imamura, Paulo Mitsuo; Melo, Leandro Vilela de; Castello, Claudio

    2009-01-01

    A synthesis of (+)-α-terpineol from (+)-limonene was proposed as a project for undergraduate organic laboratory course. Terpineol is a useful flavor and fragrance compound, and several aspects of this preparation are suited for experimental organic classes, including basic techniques for extraction and analyses of essential oils, different reaction types and the possibility of a high degree of student interest. (author)

  9. Mineral and chemical composition of rock core and surface gas composition in Horonobe Underground Research Laboratory project. Phase 1

    International Nuclear Information System (INIS)

    Hiraga, Naoto; Ishii, Eiichi

    2008-02-01

    The following three kinds of analyses were conducted for the 1st phase of the Horonobe Underground Research Laboratory Project. Mineral composition analysis of core sample. Whole rock chemical composition analysis of core sample. Surface gas composition analysis. This document summarizes the results of these analyses. (author)

  10. Data of groundwater from boreholes, river water and precipitation for the Horonobe Underground Research Laboratory project. 2011-2010

    International Nuclear Information System (INIS)

    Amano, Yuki; Yamamoto, Yoichi; Nanjyo, Isao; Murakami, Hiroaki; Yokota, Hideharu; Yamazaki, Masanori; Iwatsuki, Teruki; Kunimaru, Takanori; Oyama, Takahiro

    2012-02-01

    In the Horonobe Underground Research Laboratory (URL) Project, groundwater from boreholes, river water and precipitation have been analyzed for the environmental monitoring since the fiscal year 2001. This report shows the data set of water chemistry since the fiscal year 2001 to the fiscal year 2010. (author)

  11. Biology of Amblyomma aureolatum (Pallas, 1772 (Acari: Ixodidae on some laboratory hosts in Brazil

    Directory of Open Access Journals (Sweden)

    Daniel Sobreira Rodrigues

    2002-09-01

    Full Text Available The ixodid Amblyomma aureolatum is suspected to play a role in the epidemiology of wild life-cycle hemoparasites, which frequently infect dogs in rural and hunting areas in Brazil. Little is known about its bionomics. The objective of the present study was to evaluate some bionomic aspects of A. aureolatum ticks in Brazil. One engorged female, collected from a dog (Canis familiaris in São Sebastião das Águas Claras, State of Minas Gerais, was used to establish a colony in the laboratory. Subsequently its parasitic stage progeny were fed on domestic dogs and laboratory animals. The free-living stages were incubated at 27ºC ± 2°C and minimum 70% relative humidity in a BOD incubator. The egg incubation period ranged from 31 to 34 days; the parasitic period of larvae ranged from 4 to 6 days and ecdysis to nymphs occurred from day 19 up to day 22. The parasitic period of nymphs ranged from 5 to 8 days and the period of ecdysis to adults from 31 to 33 days. The parasitic period of adults ranged from 11 to 15 days, the pre-oviposition period from 6 to 12 days, and the oviposition period from 9 to 38 days. The total duration of the life cycle ranged from 116 to 168 days.

  12. Influence of volunteer and project characteristics on data quality of biological surveys.

    Science.gov (United States)

    Lewandowski, Eva; Specht, Hannah

    2015-06-01

    Volunteer involvement in biological surveys is becoming common in conservation and ecology, prompting questions on the quality of data collected in such surveys. In a systematic review of the peer-reviewed literature on the quality of data collected by volunteers, we examined the characteristics of volunteers (e.g., age, prior knowledge) and projects (e.g., systematic vs. opportunistic monitoring schemes) that affect data quality with regards to standardization of sampling, accuracy and precision of data collection, spatial and temporal representation of data, and sample size. Most studies (70%, n = 71) focused on the act of data collection. The majority of assessments of volunteer characteristics (58%, n = 93) examined the effect of prior knowledge and experience on quality of the data collected, often by comparing volunteers with experts or professionals, who were usually assumed to collect higher quality data. However, when both groups' data were compared with the same accuracy standard, professional data were more accurate in only 4 of 7 cases. The few studies that measured precision of volunteer and professional data did not conclusively show that professional data were less variable than volunteer data. To improve data quality, studies recommended changes to survey protocols, volunteer training, statistical analyses, and project structure (e.g., volunteer recruitment and retention). © 2015, Society for Conservation Biology.

  13. A Synthetic Biology Project - Developing a single-molecule device for screening drug-target interactions.

    Science.gov (United States)

    Firman, Keith; Evans, Luke; Youell, James

    2012-07-16

    This review describes a European-funded project in the area of Synthetic Biology. The project seeks to demonstrate the application of engineering techniques and methodologies to the design and construction of a biosensor for detecting drug-target interactions at the single-molecule level. Production of the proteins required for the system followed the principle of previously described "bioparts" concepts (a system where a database of biological parts - promoters, genes, terminators, linking tags and cleavage sequences - is used to construct novel gene assemblies) and cassette-type assembly of gene expression systems (the concept of linking different "bioparts" to produce functional "cassettes"), but problems were quickly identified with these approaches. DNA substrates for the device were also constructed using a cassette-system. Finally, micro-engineering was used to build a magnetoresistive Magnetic Tweezer device for detection of single molecule DNA modifying enzymes (motors), while the possibility of constructing a Hall Effect version of this device was explored. The device is currently being used to study helicases from Plasmodium as potential targets for anti-malarial drugs, but we also suggest other potential uses for the device. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  14. Data management strategies for multinational large-scale systems biology projects.

    Science.gov (United States)

    Wruck, Wasco; Peuker, Martin; Regenbrecht, Christian R A

    2014-01-01

    Good accessibility of publicly funded research data is essential to secure an open scientific system and eventually becomes mandatory [Wellcome Trust will Penalise Scientists Who Don't Embrace Open Access. The Guardian 2012]. By the use of high-throughput methods in many research areas from physics to systems biology, large data collections are increasingly important as raw material for research. Here, we present strategies worked out by international and national institutions targeting open access to publicly funded research data via incentives or obligations to share data. Funding organizations such as the British Wellcome Trust therefore have developed data sharing policies and request commitment to data management and sharing in grant applications. Increased citation rates are a profound argument for sharing publication data. Pre-publication sharing might be rewarded by a data citation credit system via digital object identifiers (DOIs) which have initially been in use for data objects. Besides policies and incentives, good practice in data management is indispensable. However, appropriate systems for data management of large-scale projects for example in systems biology are hard to find. Here, we give an overview of a selection of open-source data management systems proved to be employed successfully in large-scale projects.

  15. Mizunami Underground Research Laboratory project. A project on research stage of investigating prediction from ground surface. Project report at fiscal year of 2000 to 2004

    International Nuclear Information System (INIS)

    2000-04-01

    This was a detailed plan after fiscal year 2000 on the first stage of the Research stage at investigating prediction from ground surface' in three researches carried out at the Mizunami Underground Research Laboratory (MIU) according to the 'Basic plan on research of underground science at MIU', based on progress of investigation and research before fiscal year 1999. This project contains following three items as its general targets; establishment of general investigating techniques for geological environment, collection of informations on deep underground environment, and development on foundation of engineering technology at super-deep underground. And, targets at investigating prediction stage from ground surface contain acquisition of geological environment data through investigations from ground surface to predict changes of the environment accompanied with underground geological environment and construction of experimental tunnel, to determine evaluating method on prediction results, and to determine plannings of an investigating stage accompanied with excavation of the tunnel by carrying out detail design of the tunnel. Here were introduced about results and problems on the investigation of the first phase, the integration of investigating results, and the investigation and researches on geology/geological structure, hydrology and geochemistry of groundwater, mechanical properties of rocks, and the mass transfer. (G.K.)

  16. Edgewood Chemical Biological Center In-House Laboratory Independent Research Program Annual Report FY11

    Science.gov (United States)

    2011-01-01

    processes or products in mind ”. ECBC views the ILIR program as a critical part of its efforts to ensure a high level of basic science, foster innovation in...this project, density functional theory at the B3LYP/6-31+G(d) level was used to explore the ability of molecules structurally derived from the purine...effect on embryonic development, leading to neural tube deficits, autism , and cognitive dysfunction.[5] Cezar et al. used LC-MS to examine the

  17. Preliminary Conceptual Design Report for the FACET-II Project at SLAC National Accelerator Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hogan, Mark [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2016-04-22

    Plasma wakefield acceleration has the potential to dramatically shrink the size and cost of particle accelerators. Research at the SLAC National Accelerator Laboratory has demonstrated that plasmas can provide 1,000 times the acceleration in a given distance compared with current technologies. Developing revolutionary and more efficient acceleration techniques that allow for an affordable high-energy collider is the focus of FACET, a National User Facility at SLAC. The existing FACET National User Facility uses part of SLAC’s two-mile-long linear accelerator to generate high-density beams of electrons and positrons. FACET-II is a new test facility to develop advanced acceleration and coherent radiation techniques with high-energy electron and positron beams. It is the only facility in the world with high energy positron beams. FACET-II provides a major upgrade over current FACET capabilities and the breadth of the potential research program makes it truly unique. It will synergistically pursue accelerator science that is vital to the future of both advanced acceleration techniques for High Energy Physics, ultra-high brightness beams for Basic Energy Science, and novel radiation sources for a wide variety of applications. The design parameters for FACET-II are set by the requirements of the plasma wakefield experimental program. To drive the plasma wakefield requires a high peak current, in excess of 10kA. To reach this peak current, the electron and positron design bunch size is 10μ by 10μ transversely with a bunch length of 10μ. This is more than 200 times better than what has been achieved at the existing FACET. The beam energy is 10 GeV, set by the Linac length available and the repetition rate is up to 30 Hz. The FACET-II project is scheduled to be constructed in three major stages. Components of the project discussed in detail include the following: electron injector, bunch compressors and linac, the positron system, the Sector 20 sailboat and W chicanes

  18. Ecological Footprint of Biological Resource Consumption in a Typical Area of the Green for Grain Project in Northwestern China

    Directory of Open Access Journals (Sweden)

    Jie Hu

    2015-01-01

    Full Text Available Following the implementation of the Green for Grain Project in 2000 in Guyuan, China, the decrease in cultivated land and subsequent increase in forest and grassland pose substantial challenges for the supply of biological products. Whether the current biologically productive land-use patterns in Guyuan satisfy the biological product requirements for local people is an urgent problem. In this study, the ecological footprints of biological resource consumption in Guyuan were calculated and analyzed based on the ‘City Hectare’ Ecological Footprint (EF Method. The EFs of different types of biological resource products consumed from different types of biologically productive land were then analyzed. In addition, the EFs of various biological resource products before and after the implementation of the Green for Grain Project (1998 and 2012 were assessed. The actual EF and bio-capacity (BC were compared, and differences in the EF and BC for different types of biologically productive lands before and after the project were analyzed. The results showed that the EF of Guyuan’s biological resource products was 0.65866 ha/cap, with an EF outflow and EF inflow of 0.2280 ha/cap and 0.0951 ha/cap, respectively. The per capita EF of Guyuan significantly decreased after the project, as did the ecological deficit. Whereas the cultivated land showed a deficit, grasslands were characterized by ecological surplus. The total EF of living resource consumption in Guyuan was 810,941 ha, and the total BC was 768,065 ha. In additional to current biological production areas, approximately 42,876 ha will be needed to satisfy the demands of Guyuan’s people. Cultivated land is the main type of biologically productive land that is needed.

  19. S.A.P. Students Adopt Plants: A Curriculum Guide for Independent Research Projects in High School Biology.

    Science.gov (United States)

    Wilkinson, Gayle A.

    This curriculum guide begins with classroom and text study of plants and develops into an individual research project that continues throughout the school year outside the regular biology or botany teaching plan and text. The project uses about one class period every 2 weeks for group discussions, evaluations, and suggestions for the individual…

  20. The Mizunami Underground Research Laboratory Project. A fiscal year program (at fiscal year 2001). Technical report

    International Nuclear Information System (INIS)

    2001-06-01

    Study on stratum science in the Mizunami Underground Research Laboratory (MIU) Project is planned to classify it to the following three steps to progress them by considering some differences such as construction process, subject/object/scale and so on of its survey research accompanied with it in facilities in the MIU; 1) A study step on survey forecasting from earth surface, 2) A study step accompanied with excavation of road for study, and 3) A study step using the road for study. In fiscal year 2001, a trial drilling survey at No. MIU04 hole and a long-term water pumping test in the research items at objects of a series of processes on survey, analysis and evaluation, are planned to carry out. The trial survey is planned to finish at early half of the fiscal year, and its report will be summarized after analysis and evaluation of the trial survey at the No. MIU-4 hole and comparison and evaluation with already made geological environment models. According to these results, by carrying out some investigations on an engineering plan and detailed survey and research plan at the second step, renewal of the engineering plan on the road for study from later half of fiscal year 2001 to fiscal year 2002 and preparation of a basic flow on survey/analysis/evaluation of the second step will be progressed. And, as the long-term water pumping test is planned to be carried out at later half of fiscal year 2001, so its analysis and evaluation are planned to carry continuously out to fiscal year 2002. According to these results, after fiscal year 2002, renewal of engineering plan on the road for study and preparation of detailed survey and research plan at the second step will be progressed. (G.K.)

  1. Yucca Mountain Project - Argonne National Laboratory annual progress report, FY 1994

    Energy Technology Data Exchange (ETDEWEB)

    Bates, J.K.; Fortner, J.A.; Finn, P.A.; Wronkiewicz, D.J.; Hoh, J.C.; Emery, J.W.; Buck, E.C.; Wolf, S.F.

    1995-02-01

    This document reports on the work done by the Nuclear Waste Management Section of the Chemical Technology Division (CMT), Argonne National Laboratory, in the period October 1993-September 1994. Studies have been performed to evaluate the performance of nuclear waste glass and spent fuel samples under unsaturated conditions (low volume water contact) that are likely to exist in the Yucca Mountain environment being considered as a potential site for a high-level waste repository. Tests with simulated waste glasses have been in progress for over eight years and demonstrate that actinides from initially fresh glass surfaces will be released as a result of the spallation of reacted glass layers from the surface, as the small volume of water passes over the waste form. Studies are also underway to evaluate the performance of spent fuel samples and unirradiated UO{sub 2} in projected repository conditions. Tests with UO{sub 2} have been ongoing for nine years and show that the oxidation of UO{sub 2} occurs rapidly, and the resulting paragenetic sequence of secondary phases that form on the sample surface is similar to that observed in natural analogues. The reaction of spent fuel samples under conditions similar to those used with UO{sub 2} have been in progress for nearly two years, and the results suggest that spent fuel follows the same reaction progress as UO{sub 2}. The release of individual fission products and transuranic elements was not congruent, with the release being controlled by the formation of small particles or colloids that are suspended in solution and transported away from the waste form. The reaction progress depends on the composition of the spent fuel samples used and, likely, on the composition of the groundwater that contacts the waste form.

  2. IMAGE Project: Results of Laboratory Tests on Tracers for Supercritical Conditions.

    Science.gov (United States)

    Brandvoll, Øyvind; Opsahl Viig, Sissel; Nardini, Isabella; Muller, Jiri

    2016-04-01

    The use of tracers is a well-established technique for monitoring dynamic behaviour of water and gas through a reservoir. In geothermal reservoirs special challenges are encountered due to high temperatures and pressures. In this work, tracer candidates for monitoring water at supercritical conditions (temperature > 374°C, pressure ca 218 bar), are tested in laboratory experiments. Testing of tracers at supercritical water conditions requires experimental set-ups which tolerate harsh conditions with respect to high temperature and pressure. In addition stringent HES (health, environment and safety) factors have to be taken into consideration when designing and performing the experiments. The setup constructed in this project consists of a pressure vessel, high pressure pump, instrumentation for pressure and temperature control and instrumentation required for accurate sampling of tracers. In order to achieve accurate results, a special focus has been paid to the development of the tracer sampling technique. Perfluorinated cyclic hydrocarbons (PFCs) have been selected as tracer candidates. This group of compounds is today commonly used as gas tracers in oil reservoirs. According to the literature they are stable at temperatures up to 400°C. To start with, five PFCs have been tested for thermal stability in static experiments at 375°C and 108 bar in the experimental setup described above. The tracer candidates will be further tested for several months at the relevant conditions. Preliminary results indicate that some of the PFC compounds show stability after three months. However, in order to arrive at conclusive results, the experiments have to be repeated over a longer period and paying special attention to more accurate sampling procedures.

  3. ICPP injection well alternative project, Idaho National Engineering Laboratory. Final report

    International Nuclear Information System (INIS)

    1980-10-01

    The Idaho Chemical Processing Plant (ICPP) portion of the Idaho National Engineering Laboratory (INEL) has been obtaining water needed for its operations from the Snake River aquifer, which occupies the entire region underlying the site. Most of this water has been used for cooling operating equipment, while a small portion has found various process uses. After passing through the ICPP process area, these waters are then returned to the aquifer. A small portion (about 1%) of the returned stream contains measurable amounts of radioactivity derived from the miscellaneous process users. This report and the recommendations contained herein are based upon stream flows projected for 1985 as supplied by DOE for the ICPP. 26 different alternatives for handling cooling water, chemical, and low level radioactive water disposal are examined. These cases are considered from technical, environmental, safety, and economic points of view. The level of detail is sufficient to eliminate non-viable cases, and to identify those which offer improvements over present practice. The Environmental/Safety Risk Factors were evaluated on a qualitative comparison basis only. Before a recommended improvement is incorporated into the waste disposal system, a conceptual design study should be made which would evaluate all those secondary effects and environmental factors that, by the very nature of the screening process, this study has not provided. Certain synergistic combinations have been noted and are discussed. This report does note whether the operations considered are in regulatory compliance, or are likely to be capable of providing lasting improvement to the waste water system. Qualitative comparisons were made between the various alternatives to confirm their relationship with applicable standards

  4. Remediation Approach for the Integrated Facility Disposition Project at the Oak Ridge National Laboratory

    International Nuclear Information System (INIS)

    Kirk, P.G.; Stephens, Jr.J.M.

    2009-01-01

    The Integrated Facility Disposition Project (IFDP) is a multi-billion-dollar remediation effort being conducted by the U.S. Department of Energy (DOE) Office of Environmental Management in Oak Ridge, Tennessee. The scope of the IFDP encompasses remedial actions related to activities conducted over the past 65 years at the Oak Ridge National Laboratory (ORNL) and the Y-12 National Security Complex (Y-12). Environmental media and facilities became contaminated as a result of operations, leaks, spills, and past waste disposal practices. ORNL's mission includes energy, environmental, nuclear security, computational, and materials research and development. Remediation activities will be implemented at ORNL as part of IFDP scope to meet remedial action objectives established in existing and future decision documents. Remedial actions are necessary (1) to comply with environmental regulations to reduce human health and environmental risk and (2) to release strategic real estate needed for modernization initiatives at ORNL. The scope of remedial actions includes characterization, waste management, transportation and disposal, stream restoration, and final remediation of contaminated soils, sediments, and groundwater. Activities include removal of at or below-grade substructures such as slabs, underground utilities, underground piping, tanks, basins, pits, ducts, equipment housings, manholes, and concrete-poured structures associated with equipment housings and basement walls/floors/columns. Many interim remedial actions involving groundwater and surface water that have not been completed are included in the IFDP remedial action scope. The challenges presented by the remediation of Bethel Valley at ORNL are formidable. The proposed approach to remediation endeavors to use the best available technologies and technical approaches from EPA and other federal agencies and lessons learned from previous cleanup efforts. The objective is to minimize cost, maximize remedial

  5. Practice makes pretty good: assessment of primary literature reading abilities across multiple large-enrollment biology laboratory courses.

    Science.gov (United States)

    Sato, Brian K; Kadandale, Pavan; He, Wenliang; Murata, Paige M N; Latif, Yama; Warschauer, Mark

    2014-01-01

    Primary literature is essential for scientific communication and is commonly utilized in undergraduate biology education. Despite this, there is often little time spent training our students how to critically analyze a paper. To address this, we introduced a primary literature module in multiple upper-division laboratory courses. In this module, instructors conduct classroom discussions that dissect a paper as researchers do. While previous work has identified classroom interventions that improve primary literature comprehension within a single course, our goal was to determine whether including a scientific paper module in our classes could produce long-term benefits. On the basis of performance in an assessment exam, we found that our module resulted in longitudinal gains, including increased comprehension and critical-thinking abilities in subsequent lab courses. These learning gains were specific to courses utilizing our module, as no longitudinal gains were seen in students who had taken other upper-division labs that lacked extensive primary literature discussion. In addition, we assessed whether performance on our assessment correlated with a variety of factors, including grade point average, course performance, research background, and self-reported confidence in understanding of the article. Furthermore, all of the study conclusions are independent of biology disciplines, as we observe similar trends within each course. © 2014 B. K. Sato et al. CBE—Life Sciences Education © 2014 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  6. Biology of Anicla infecta (Ochsenheimer, 1816 (Lepidoptera, Noctuidae, Noctuinae, under laboratory conditions

    Directory of Open Access Journals (Sweden)

    J. A. TESTON

    Full Text Available Larvae of Anicla infecta (Ochsenheimer, 1816 (Noctuidae feed upon many grasses and may be harmful to cereals and fodder of economic importance. This study was developed aiming to contribute to knowledge of the biology of this species. The rearing was done in an environmental chamber with the following settings: temperature of 25 ± 1ºC; relative humidity of 70% ± 10%, and photoperiod of L14: D10. The larvae fed on ryegrass, Lolium multiflorum Lam. The results express the mean and standard error for the length of every stage in days. For each stage we observed the following time of development: egg 3.2 ± 0.09; larvae 18.7 ± 0.07; pre-pupae 3.3 ± 0.04; pupae 12.6 ± 0.14; and adult longevity was 12.1 ± 1.03. Also the pre-egg-laying period was 4.4 ± 0.59; the egg-laying period was 8.1 ± 0.84; and the post-egg-laying period was 0.3 ± 0.14. The mean number of egg-laying cycles per female was 6.7 ± 0.73; that of eggs per cycle was 77.5 ± 4.37; and total eggs per female was 521.4 ± 47.36.

  7. Bio Engineering Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Description/History: Chemistry and biology laboratoriesThe Bio Engineering Laboratory (BeL) is theonly full spectrum biotechnology capability within the Department...

  8. A Brief History of the Study of Fish Osmoregulation: The Central Role of the Mt. Desert Island Biological Laboratory

    Science.gov (United States)

    Evans, David H.

    2010-01-01

    The Mt. Desert Island Biological Laboratory (MDIBL) has played a central role in the study of fish osmoregulation for the past 80 years. In particular, scientists at the MDIBL have made significant discoveries in the basic pattern of fish osmoregulation, the function of aglomerular kidneys and proximal tubular secretion, the roles of NaCl cotransporters in intestinal uptake and gill and rectal gland secretion, the role of the shark rectal gland in osmoregulation, the mechanisms of salt secretion by the teleost fish gill epithelium, and the evolution of the ionic uptake mechanisms in fish gills. This short review presents the history of these discoveries and their relationships to the study of epithelial transport in general. PMID:21423356

  9. High-resolution emission tomography of small laboratory animals: physics and gamma-astronomy meet molecular biology

    International Nuclear Information System (INIS)

    Beekman, F.J.; Colijn, A.P.; Vastenhouw, B.; Wiegant, V.M.; Gerrits, M.A.F.M.

    2003-01-01

    Molecular imaging can be defined as the characterization and measurement of biological processes in living animals, model systems and humans at the cellular and molecular level using remote imaging detectors. An example concerns the mapping of the distributions of radioactively labeled molecules in laboratory animals which is of crucial importance for life sciences. Tomographic methods like Single Photon Emission Computed Tomography (SPECT) offer a possibility to visualize distributions of radioactively labeled molecules in living animals. Miniature tomography systems, derived from their clinical counterparts, but with a much higher image resolution are under development in several institutes. An example is U-SPECT that will be discussed in the present paper. Such systems are expected to accelerate several biomedical research procedures, the understanding of gene and protein function, as well as pharmaceutical development

  10. Laboratory and field studies related to the radionuclide migration project. Progress report, October 1, 1982-September 30, 1983

    International Nuclear Information System (INIS)

    Daniels, W.R.; Thompson, J.L.

    1984-04-01

    The FY 1983 laboratory and field studies related to the Radionuclide Migration project are described. Results are presented for radiochemical analyses of water samples collected from the RNM-1 well and the RNM-2S satellite well at the Cambric site. Data are included for tritium, 36 Cl, 85 Kr, 90 Sr, 129 I, and 137 Cs. Preliminary results from water collection at the Cheshire site are reported. Laboratory studies emphasize the sorptive behavior of tuff and its dependence on mineralogy. 18 references, 7 figures, 13 tables

  11. A CMOS active pixel sensor system for laboratory- based x-ray diffraction studies of biological tissue

    International Nuclear Information System (INIS)

    Bohndiek, Sarah E; Cook, Emily J; Arvanitis, Costas D; Olivo, Alessandro; Royle, Gary J; Clark, Andy T; Prydderch, Mark L; Turchetta, Renato; Speller, Robert D

    2008-01-01

    X-ray diffraction studies give material-specific information about biological tissue. Ideally, a large area, low noise, wide dynamic range digital x-ray detector is required for laboratory-based x-ray diffraction studies. The goal of this work is to introduce a novel imaging technology, the CMOS active pixel sensor (APS) that has the potential to fulfil all these requirements, and demonstrate its feasibility for coherent scatter imaging. A prototype CMOS APS has been included in an x-ray diffraction demonstration system. An industrial x-ray source with appropriate beam filtration is used to perform angle dispersive x-ray diffraction (ADXRD). Optimization of the experimental set-up is detailed including collimator options and detector operating parameters. Scatter signatures are measured for 11 different materials, covering three medical applications: breast cancer diagnosis, kidney stone identification and bone mineral density calculations. Scatter signatures are also recorded for three mixed samples of known composition. Results are verified using two independent models for predicting the APS scatter signature: (1) a linear systems model of the APS and (2) a linear superposition integral combining known monochromatic scatter signatures with the input polychromatic spectrum used in this case. Cross validation of experimental, modelled and literature results proves that APS are able to record biologically relevant scatter signatures. Coherent scatter signatures are sensitive to multiple materials present in a sample and provide a means to quantify composition. In the future, production of a bespoke APS imager for x-ray diffraction studies could enable simultaneous collection of the transmitted beam and scattered radiation in a laboratory-based coherent scatter system, making clinical transfer of the technique attainable

  12. 75 FR 27865 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-05-18

    ... contributors;'' and (d) increased employee satisfaction with the Laboratory. D. Participating Employees and.... This disrupts the R&D process and reduces the Laboratory's ability to serve its customers. Under the... simply defined as the measure of the demonstrated value of what an employee did in terms of accomplishing...

  13. 78 FR 29335 - Science and Technology Reinvention Laboratory (STRL) Personnel Management Demonstration Projects

    Science.gov (United States)

    2013-05-20

    ...) Personnel Management Demonstration Projects AGENCY: Office of the Deputy Assistant Secretary of Defense... demonstration project plans. SUMMARY: Section 342(b) of the National Defense Authorization Act (NDAA) for Fiscal... 2001, authorizes the Secretary of Defense to conduct personnel demonstration projects at DoD...

  14. 77 FR 69601 - Science and Technology Reinvention Laboratory (STRL) Personnel Management Demonstration Projects

    Science.gov (United States)

    2012-11-20

    ...) Personnel Management Demonstration Projects AGENCY: Office of the Deputy Assistant Secretary of Defense... to demonstration project plans. SUMMARY: Section 342(b) of the National Defense Authorization Act... the NDAA for FY 2001, authorizes the Secretary of Defense to conduct personnel demonstration projects...

  15. 75 FR 77379 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-12-10

    ... Personnel Management Demonstration Project, Department of Navy, Office of Naval Research; Notice #0;#0..., authorizes the Secretary of Defense (SECDEF) to conduct personnel management demonstration projects at... to execute a process and plan to employ the Department's personnel management demonstration project...

  16. 75 FR 55199 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-09-09

    ... customers; and 7. Workforce satisfaction. An evaluation model was developed for the Director, Defense... personnel system under an appropriate demonstration project as defined in section 342(b) of Public Law 103... satisfaction. With some modifications, this project mirrors the STRL personnel management demonstration project...

  17. From Never Born Proteins to Minimal Living Cells: two projects in synthetic biology.

    Science.gov (United States)

    Luisi, Pier Luigi; Chiarabelli, Cristiano; Stano, Pasquale

    2006-12-01

    The Never Born Proteins (NBPs) and the Minimal Cell projects are two currently developed research lines belonging to the field of synthetic biology. The first deals with the investigation of structural and functional properties of de novo proteins with random sequences, selected and isolated using phage display methods. The minimal cell is the simplest cellular construct which displays living properties, such as self-maintenance, self-reproduction and evolvability. The semi-synthetic approach to minimal cells involves the use of extant genes and proteins in order to build a supramolecular construct based on lipid vesicles. Results and outlooks on these two research lines are shortly discussed, mainly focusing on their relevance to the origin of life studies.

  18. The NYC native air sampling pilot project: using HVAC filter data for urban biological incident characterization.

    Science.gov (United States)

    Ackelsberg, Joel; Leykam, Frederic M; Hazi, Yair; Madsen, Larry C; West, Todd H; Faltesek, Anthony; Henderson, Gavin D; Henderson, Christopher L; Leighton, Terrance

    2011-09-01

    Native air sampling (NAS) is distinguished from dedicated air sampling (DAS) devices (eg, BioWatch) that are deployed to detect aerosol disseminations of biological threat agents. NAS uses filter samples from heating, ventilation, and air conditioning (HVAC) systems in commercial properties for environmental sampling after DAS detection of biological threat agent incidents. It represents an untapped, scientifically sound, efficient, widely distributed, and comparably inexpensive resource for postevent environmental sampling. Calculations predict that postevent NAS would be more efficient than environmental surface sampling by orders of magnitude. HVAC filter samples could be collected from pre-identified surrounding NAS facilities to corroborate the DAS alarm and delineate the path taken by the bioaerosol plume. The New York City (NYC) Native Air Sampling Pilot Project explored whether native air sampling would be acceptable to private sector stakeholders and could be implemented successfully in NYC. Building trade associations facilitated outreach to and discussions with property owners and managers, who expedited contact with building managers of candidate NAS properties that they managed or owned. Nominal NAS building requirements were determined; procedures to identify and evaluate candidate NAS facilities were developed; data collection tools and other resources were designed and used to expedite candidate NAS building selection and evaluation in Manhattan; and exemplar environmental sampling playbooks for emergency responders were completed. In this sample, modern buildings with single or few corporate tenants were the best NAS candidate facilities. The Pilot Project successfully demonstrated that in one urban setting a native air sampling strategy could be implemented with effective public-private collaboration.

  19. Biologic surveys for the Sandia National Laboratories, Coyote Canyon Test Complex, Kirtland Air Force Base, Albuquerque, New Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, R.M. [4115 Allen Dr., Kingsville, TX (United States); Knight, P.J. [Marron and Associates, Inc., Corrales, NM (United States)

    1994-05-25

    This report provides results of a comprehensive biologic survey performed in Coyote Canyon Test Complex (CCTC), Sandia National Laboratories (SNL), Bernalillo County, New Mexico, which was conducted during the spring and summer of 1992 and 1993. CCTC is sited on land owned by the Department of Energy (DOE) and Kirtland Air Force Base and managed by SNL. The survey covered 3,760 acres of land, most of which is rarely disturbed by CCTC operations. Absence of grazing by livestock and possibly native ungulates, and relative to the general condition of private range lands throughout New Mexico, and relative to other grazing lands in central New Mexico. Widely dispersed, low intensity use by SNL as well as prohibition of grazing has probably contributed to abundance of special status species such as grama grass cactus within the CCTC area. This report evaluates threatened and endangered species found in the area, as well as comprehensive assessment of biologic habitats. Included are analyses of potential impacts and mitigative measures designed to reduce or eliminate potential impacts. Included is a summary of CCTC program and testing activities.

  20. SensorDB: a virtual laboratory for the integration, visualization and analysis of varied biological sensor data.

    Science.gov (United States)

    Salehi, Ali; Jimenez-Berni, Jose; Deery, David M; Palmer, Doug; Holland, Edward; Rozas-Larraondo, Pablo; Chapman, Scott C; Georgakopoulos, Dimitrios; Furbank, Robert T

    2015-01-01

    To our knowledge, there is no software or database solution that supports large volumes of biological time series sensor data efficiently and enables data visualization and analysis in real time. Existing solutions for managing data typically use unstructured file systems or relational databases. These systems are not designed to provide instantaneous response to user queries. Furthermore, they do not support rapid data analysis and visualization to enable interactive experiments. In large scale experiments, this behaviour slows research discovery, discourages the widespread sharing and reuse of data that could otherwise inform critical decisions in a timely manner and encourage effective collaboration between groups. In this paper we present SensorDB, a web based virtual laboratory that can manage large volumes of biological time series sensor data while supporting rapid data queries and real-time user interaction. SensorDB is sensor agnostic and uses web-based, state-of-the-art cloud and storage technologies to efficiently gather, analyse and visualize data. Collaboration and data sharing between different agencies and groups is thereby facilitated. SensorDB is available online at http://sensordb.csiro.au.

  1. HIGH LEVEL WASTE TANK CLOSURE PROJECT AT THE IDAHO NATIONAL ENGINEERING AND ENVIRONMENTAL LABORATORY

    International Nuclear Information System (INIS)

    Quigley, K.D.; Wessman, D.

    2003-01-01

    The Department of Energy, Idaho Operations Office (DOE-ID) is in the process of closing two underground high-level waste (HLW) storage tanks at the Idaho National Engineering and Environmental Laboratory (INEEL) to meet Resource Conservation and Recovery Act (RCRA) regulations and Department of Energy orders. Closure of these two tanks is scheduled for 2004 as the first phase in closure of the eleven 1.14 million liter (300,000 gallon) tanks currently in service at the Idaho Nuclear Technology and Engineering Center (INTEC). The INTEC Tank Farm Facility (TFF) Closure sequence consists of multiple steps to be accomplished through the existing tank riser access points. Currently, the tank risers contain steam and process waste lines associated with the steam jets, corrosion coupons, and liquid level indicators. As necessary, this equipment will be removed from the risers to allow adequate space for closure equipment and activities. The basic tank closure sequence is as follows: Empty the tank to the residual heel using the existing jets; Video and sample the heel; Replace steam jets with new jet at a lower position in the tank, and remove additional material; Flush tank, piping and secondary containment with demineralized water; Video and sample the heel; Evaluate decontamination effectiveness; Displace the residual heel with multiple placements of grout; and Grout piping, vaults and remaining tank volume. Design, development, and deployment of a remotely operated tank cleaning system were completed in June 2002. The system incorporates many commercially available components, which have been adapted for application in cleaning high-level waste tanks. The system is cost-effective since it also utilizes existing waste transfer technology (steam jets), to remove tank heel solids from the tank bottoms during the cleaning operations. Remotely operated directional spray nozzles, automatic rotating wash balls, video monitoring equipment, decontamination spray-rings, and

  2. Progress Toward a Thermal-Hydrological-Mechanical-Chemical-Biological (THMCB) Experiment in the Homestake Mine Deep Underground Science and Engineering Laboratory

    Science.gov (United States)

    Sonnenthal, E. L.; Maher, K.; Elsworth, D.; Lowell, R. P.; Uzunlar, N.; Mailloux, B. J.; Conrad, M. E.; Olsen, N. J.; Jones, T. L.; Cruz, M. F.; Torchinsky, A.

    2011-12-01

    The purpose of performing a long-term hydrothermal experiment in a deep mine is to gain a scientific understanding of the coupled physical, chemical, and biological processes taking place in fractured rock under the influence of mechanical stress, thermal effects, and fluid flow. Only in a controlled experiment in a well-characterized rock mass, can a fractured rock be probed in 3-D through geophysical imaging, in situ measurements, geochemical/biological sampling, and numerical modeling. Our project is focused on the feasibility of a THMCB experiment in the Homestake Mine, South Dakota to study the long-term evolution (10+ years) of a perturbed heterogeneous rock mass. In addition to the experiment as a laboratory for studying crustal processes, it has direct application to Enhanced Geothermal Systems, carbon sequestration, and contaminant transport. Field activities have focused on fracture and feature mapping, flux measurements from flowing fractures, and collection of water and rock samples for geochemical, biological, and isotopic analyses. Fracture mapping and seepage measurements are being used to develop estimates of permeability and fluxes at different length scales and design the location and orientation of the heater array. Fluxes measured up to several liters/minute indicate localized regions of very high fracture permeability, likely in excess of 10-10 m2. Isotopic measurements indicate heterogeneity in the fracture network on the scale of tens of meters in addition to the large-scale geochemical heterogeneity observed in the mine. New methods for sampling and filtering water samples were developed and tested with the goal of performing radiocarbon analyses in DNA and phospholipid fatty acids. Analytical and numerical models of the thermal perturbation have been used to design the heater orientation and spacing. Reaction path and THC simulations were performed to assess geochemical and porosity/permeability changes as a function of the heat input

  3. Physical, biological and clinical basis of light ions using in radiotherapy: EULIMA project

    International Nuclear Information System (INIS)

    Chauvel, P.

    1991-01-01

    Improving the efficiency of radiotherapy is a constant concern in oncology: more than half of the patients who contract cancer receive radiotherapy at some stage. Use of charged particles in radiotherapy represents indisputable progress in localization of the dose delivered to tumour masses, thereby allowing reduction of dose received by adjacent healthy tissues. Protons improve the physical selectivity of the irradiation, i.e. the dose distribution. High-LET (Linear Energy Transfer) radiations produce different biological effects, decreasing the differences in radiosensitivity, and allowing radiation therapy to control radioresistant tumours. Fast neutrons represent the most known of these high-LET particles, but they suffer of a relatively poor physical selectivity. The two approaches (physical selectivity and biological advantages) are joined in by light ions (Carbon, Oxygen, Neon). Highly selective high-LET radiation therapy can be performed for radioresistant tumours without damage to healthy tissues. Preliminary results obtained in Berkeley (USA) demonstrate an improved local control of unresectable, slowly growing tumours, confirming what could be extrapolated from proton and neutrontherapy. Furthermore, radioactive light ion beams can be used to verify the accuracy of treatment planning by checking the range of the particle with a PET camera, and in the future for the treatment itself. In the framework of its programme Europe against Cancer, the Commission of the European Communities participates in the funding of the EULIMA (European Light Ion Medical Accelerator) project feasibility study, aiming to design an hospital-based light ion therapy facility in Europe [fr

  4. Compilation of selected deep-sea biological data for the US subseabed disposal project

    International Nuclear Information System (INIS)

    Gomez, L.S.; Marietta, M.G.; Jackson, D.W.

    1987-03-01

    The US Subseabed Disposal Project (SDP) has compiled an extensive deep-sea biological data base to be used in calculating biological parameters of state and rate included in mathematical models of oceanographic transport of radionuclides. The data base is organized around a model deep-sea ecosystem which includes the following components: zooplankton, fish and other nekton, invertebrate benthic megafauna, benthic macrofauna, benthic meiofauna, heterotrophic microbiota, as well as suspended and sediment particulate organic carbon. Measurements of abundance and activity rates (e.g., respiration, production, sedimentation, etc.) reported in the international oceanographic literature are summarized in 23 tables. Included in these tables are the latitudinal position of the studies, as well as information describing sampling techniques and any special notes needed to better assess the data presented. This report has been prepared primarily as a resource document to be used in calculating parameter values for various modeling applications, and for preparing historical data reviews for other SDP reports. Depending on the intended use, these data will require further reduction and unit conversion

  5. Hydrogeological characterization on surface-based investigation phase in the Mizunami underground research laboratory project, in Japan

    International Nuclear Information System (INIS)

    Saegusa, Hiromitsu; Onoe, Hironori; Takeuchi, Shinji; Takeuchi, Ryuji; Ohyama, Takuya

    2007-01-01

    The Mizunami Underground Research Laboratory (MIU) project is being carried out by Japan Atomic Energy Agency in the Cretaceous Toki granite in the Tono area, central Japan. The MIU project is a purpose-built generic underground research laboratory project that is planned for a broad scientific study of the deep geological environment as a basis of research and development for geological disposal of nuclear wastes. One of the main goals of the MIU project is to establish comprehensive techniques for investigation, analysis, and assessment of the deep geological environment. The MIU project has three overlapping phases: Surface-based Investigation (Phase I), Construction (Phase II) and Operation (Phase III). Hydrogeological investigations using a stepwise process in Phase I have been carried out in order to obtain information on important properties such as, location of water conducting features, hydraulic conductivity and so on. Hydrogeological modeling and groundwater flow simulations in Phase I have been carried out in order to synthesize these investigation results, to evaluate the uncertainty of the hydrogeological model and to identify the main issues for further investigations. Using the stepwise hydrogeological characterization approach and combining the investigation with modeling and simulation, understanding of the hydrogeological environment has been progressively improved. (authors)

  6. New Ideas for an Old Enzyme: A Short, Question-Based Laboratory Project for the Purification and Identification of an Unknown LDH Isozyme

    Science.gov (United States)

    Coleman, Aaron B.

    2010-01-01

    Enzyme purification projects are an excellent way to introduce many aspects of protein biochemistry, but can be difficult to carry out under the constraints of a typical undergraduate laboratory course. We have designed a short laboratory project for the purification and identification of an "unknown" lactate dehydrogenase (LDH) isozyme that can…

  7. 75 FR 53075 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-08-30

    ..., 0028 Environmental Protection Specialist, 0030 Fitness and Sports Specialist, 0080 Security... mission work evolves and new competencies are needed. E. Project Design For the expansion design, the AFRL...

  8. A Study on Evaluation of the Biology Projects Submitted to the TUBITAK Secondary Education Research Projects Contest from the Bursa Region

    Science.gov (United States)

    Zeren Özer, Dilek; Güngör, Sema Nur; Özkan, Muhlis

    2015-01-01

    This study evaluates, through the employment of scientific methods and techniques, a total of 107 Biology projects submitted by secondary education students to the Bursa Region Coordinatorship of TUBITAK (a region which encompasses the municipalities of Afyonkarahisar, Balikesir, Bilecik, Canakkale, Eskisehir, Kutahya, and Yalova). The projects…

  9. 78 FR 64204 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2013-10-28

    ... Personnel Management Demonstration Project, Department of Navy, Office of Naval Research (ONR); Amendment... ONR Personnel Management Demonstration Project (75 FR 77380-77447, December 10, 2010). SUMMARY: On December 10, 2010 (75 FR 77380-77447), DoD published a notice of approval of a personnel management...

  10. 76 FR 12507 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2011-03-07

    ... customers; and 7. Workforce satisfaction with the personnel management system. An evaluation model was... personnel system under an appropriate demonstration project as defined in section 342(b) of Public Law 103... was deleted with the actual construct of pay pools to be further defined in the Demonstration Project...

  11. ExoHab Pilot Project & Field Tests for Moon-Mars Human Laboratories

    Science.gov (United States)

    Foing, Bernard

    2010-05-01

    We studied concepts for a minimal Moon-Mars habitat, in focussing on the system aspects and coordinating every different part as part an evolving architecture. We validated experimentally the Habitat and Laboratory ExoHab concept constraints during EuroGeoMars campaign in Utah desert research station (from 24 Jan. to 28 Feb. 2009) and EuroMoonMars/DOMMEX campaigns in Nov 2009 and February-April 2010. We discuss from the ILEWG ExoHab concept studies and field simulations the specifics of human exploration, with focus on habitability and human performance. In the ExoHab pilot concept project (supported by ILEWG, ESA NASA), we justify the case for a scientific and exploration outpost allowing experiments, sample analysis in laboratory (relevant to the origin and evolution of planets and life, geophysical and geo-chemical studies, astrobiology and life sciences, observation sciences, technology demonstration, resource utilisation, human exploration and settlement). In this modular concept, we consider various infra structure elements: core habitat, Extra Vehicular activity (EVA), crew mobility, energy supply, recycling module, communication, green house and food production, operations. We review some studies space agencies' architecture proposals, with landers, orbiters, rovers, habitats, surface operations and protocols. We focus on the easiest and the soonest way in settling a minimal base immediately operational in scientific experimentation and exploration, but not immediately autonomous. Through a modular concept, this outpost will be possibly evolved into a long duration or permanent base. We will analyse the possibilities of settling such a minimal base by means of the current and near term propulsion technology, as a full Ariane 5 ME carrying 1.7 T of gross payload to the surface of the Moon (Integrated Exploration Study, ESA ESTEC [1,2]). The low solar rays incidence may permit having ice in deep craters, which will be beneficial for the evolution of the

  12. Quality assurance plan for the molten salt reactor experiment Remediation Project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1998-02-01

    This Quality Assurance Plan (QAP) identifies and describes the systems utilized by Molten Salt Reactor Experiment (MSRE) Remediation Project personnel to implement the requirements and associated applicable guidance contained in the Quality Program Description, Y/QD-15 Rev. 2 (Martin Marietta Energy Systems, Inc., 1995) and Environmental Management and Enrichment Facilities Work Smart Standards. This QAP defines the quality assurance (QA) requirements applicable to all activities and operations in and directly pertinent to the MSRE Remediation Project. This QAP will be periodically reviewed, revised, and approved as necessary. This QAP identifies and describes the QA activities and procedures implemented by the various Oak Ridge National Laboratory support organizations and personnel to provide confidence that these activities meet the requirements of this project. Specific support organization (Division) quality requirements, including the degree of implementation of each, are contained in the appendixes of this plan

  13. THE DYNAMICS OF PROJECT-BASED LEARNING EXTENSION COURSES: THE “LABORATORY OF SOCIAL PROJECTS” CASE STUDY

    Directory of Open Access Journals (Sweden)

    Joao Alberto Arantes do Amaral

    2017-01-01

    Full Text Available In this case study we discuss the dynamics that drive a free-of-charge project-based learning extension course. We discuss the lessons learned in the course, “Laboratory of Social Projects.” The course aimed to teach project management skills to the participants. It was conducted from August to November of 2015, at Federal University of São Paulo (Unifesp, Osasco Campus, Brazil. The course had 72 participants (41 community members and 31 university students. The participants worked in teams of four members (on average and developed 13 projects on behalf of eight NGOs that help people in need. In our research, we followed a mixed methods approach, using unstructured questionnaires and project reports as sources of information. We made use of system thinking analysis to reveal the dynamics that unfolded during the course. Our main findings are: 1 free-of-charge extension courses can be much more challenging to manage than traditional courses; the bureaucracy of getting the university’s approval and problems related to dropouts are issues to consider; 2 the workload of the professors involved can be substantially higher than the workload of similar regular courses; 3 the use of project-based learning techniques can be very effective; 4 the courses can provide a very rich experience to the participants, promoting intense knowledge-sharing among all involved.

  14. Assessment of Energy Efficiency Project Financing Alternatives for Brookhaven National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, W. D.; Hail, John C.; Sullivan, Gregory P.

    2000-02-14

    This document provides findings and recommendations that resulted from an assessment of the Brookhaven National Laboratory by a team from Pacific Northwest National Laboratory to assess the site's potential for various alternative financing options as a means to implement energy-efficiency improvements. The assessment looked for life-cycle cost-effective energy-efficiency improvement opportunities, and through a series of staff interviews, evaluated the various methods by which these opportunities may be financed, while considering availability of funds, staff, and available financing options. This report summarizes the findings of the visit and the resulting recommendations.

  15. Evolving a lingua franca and associated software infrastructure for computational systems biology: the Systems Biology Markup Language (SBML) project.

    Science.gov (United States)

    Hucka, M; Finney, A; Bornstein, B J; Keating, S M; Shapiro, B E; Matthews, J; Kovitz, B L; Schilstra, M J; Funahashi, A; Doyle, J C; Kitano, H

    2004-06-01

    Biologists are increasingly recognising that computational modelling is crucial for making sense of the vast quantities of complex experimental data that are now being collected. The systems biology field needs agreed-upon information standards if models are to be shared, evaluated and developed cooperatively. Over the last four years, our team has been developing the Systems Biology Markup Language (SBML) in collaboration with an international community of modellers and software developers. SBML has become a de facto standard format for representing formal, quantitative and qualitative models at the level of biochemical reactions and regulatory networks. In this article, we summarise the current and upcoming versions of SBML and our efforts at developing software infrastructure for supporting and broadening its use. We also provide a brief overview of the many SBML-compatible software tools available today.

  16. A summary of the environmental restoration program Retrieval Demonstration Project at the Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    McQuary, J.

    1991-01-01

    This document summarizes the of retrieval techniques developed to excavate buried transuranic (TRU) mixed waste from the Subsurface Disposal Area (SDA). The SDA is located at the Idaho National Engineering Laboratory (INEL) in the Radioactive Waste Management Complex (RWMC). 31 refs., 1 fig

  17. Studying Human Disease Genes in "Caenorhabditis Elegans": A Molecular Genetics Laboratory Project

    Science.gov (United States)

    Cox-Paulson, Elisabeth A.; Grana, Theresa M.; Harris, Michelle A.; Batzli, Janet M.

    2012-01-01

    Scientists routinely integrate information from various channels to explore topics under study. We designed a 4-wk undergraduate laboratory module that used a multifaceted approach to study a question in molecular genetics. Specifically, students investigated whether "Caenorhabditis elegans" can be a useful model system for studying genes…

  18. Integration of a Zebrafish Research Project into a Molecular Biology Course to Support Critical Thinking and Course Content Goals

    Science.gov (United States)

    Felzien, Lisa K.

    2016-01-01

    Engaging undergraduates in research is essential for teaching them to think like scientists, and it has become a desired component of classroom and laboratory instruction. Research projects that span an entire semester expose students to a variety of concepts and techniques and allow students to use experiments to learn scientific principles,…

  19. Final Verification Success Story Using the Triad Approach at the Oak Ridge National Laboratory's Melton Valley Soils and Sediment Project

    International Nuclear Information System (INIS)

    King, D.A.; Haas, D.A.; Cange, J.B.

    2006-01-01

    The United States Environmental Protection Agency recently published guidance on the Triad approach, which supports the use of smarter, faster, and better technologies and work strategies during environmental site assessment, characterization, and cleanup. The Melton Valley Soils and Sediment Project (Project) at the Oak Ridge National Laboratory embraced this three-pronged approach to characterize contaminants in soil/sediment across the 1000-acre Melton Valley Watershed. Systematic Project Planning is the first of three prongs in the Triad approach. Management initiated Project activities by identifying key technical personnel, included regulators early in the planning phase, researched technologies, and identified available resources necessary to meet Project objectives. Dynamic Work Strategies is the second prong of the Triad approach. Core Team members, including State and Federal regulators, helped develop a Sampling and Analysis Plan that allowed experienced field managers to make real-time, in-the-field decisions and, thus, to adjust to conditions unanticipated during the planning phase. Real-time Measurement Technologies is the third and last prong of the Triad approach. To expedite decision-making, the Project incorporated multiple in-field technologies, including global positioning system equipment integrated with field screening instrumentation, magnetometers for utility clearance, and an on-site gamma spectrometer (spec) for rapid contaminant speciation and quantification. As a result of a relatively complex but highly efficient program, a Project field staff of eight collected approximately 1900 soil samples for on-site gamma spec analysis (twenty percent were also shipped for off-site analyses), 4.7 million gamma radiation measurements, 1000 systematic beta radiation measurements, and 3600 systematic dose rate measurements between July 1, 2004, and October 31, 2005. The site database previously contained results for less than 500 soil samples dating

  20. The Horonobe Underground Research Laboratory (Tentative name) Project. A program on survey and research performed from earth surface

    International Nuclear Information System (INIS)

    2001-03-01

    The Horonobe Underground Research Laboratory (Tentative name) Project under planning at Horonobe-machi by the Japan Nuclear Cycle Development Institute (JNC) is a research facility on deep underground shown in the Long-term program on research, development and application of nuclear energy (June, 1994)' (LPNE), where some researches on the deep underground targeted at sedimentary rocks are carried out. The plan on The Horonobe Underground Research Laboratory performed at Horonobe-machi' is an about 20 years plan ranging from beginning to finishing of its survey and research, which is carried out by three steps such as 'Survey and research performed from earth surface', 'Survey and research performed under excavation of road', and Survey and research performed by using the road'. The Horonobe Underground Research Laboratory is one of research facilities on deep underground shown its importance in LPNE, and carries out some researches on the deep underground at a target of the sedimentary rocks. And also The Horonobe Underground Research Laboratory confirms some technical reliability and support on stratum disposal shown in the 'Technical reliability on stratum disposal of the high level radioactive wastes. The Second Progress Report of R and D on geological disposal' summarized on November, 1999 by JNC through actual tests and researches at the deep stratum. The obtained results are intended to reflect to disposal business of The Horonobe Underground Research Laboratory and safety regulation and so on performed by the government, together with results of stratum science research, at the Tono Geoscience Center, of geological disposal R and D at the Tokai Works, or of international collaborations. For R and D at the The Horonobe Underground Research Laboratory after 2000, following subjects are shown: 1) Survey technique on long-term stability of geological environment, 2) Survey technique on geological environment, 3) Engineering technique on engineered barrier and

  1. The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story.

    Science.gov (United States)

    Pratlong, Francine; Balard, Yves; Lami, Patrick; Talignani, Loïc; Ravel, Christophe; Dereure, Jacques; Lefebvre, Michèle; Serres, Ghislaine; Bastien, Patrick; Dedet, Jean-Pierre

    2016-12-01

    We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective analysis of the technical and organizational changes that have been adopted over time to take into account the technological advances and related modifications in the collection management and quality system. The technical improvements concerned the culture and cryopreservation techniques, strain identification by isoenzymatic and molecular techniques, data computerization and quality management to meet the changes in international standards, and in the cryogenic and microbiological safety procedures. The BRC is working toward obtaining the NF-S 96-900 certification in the coming years. Our long-term expertise in Leishmania storage and typing and collection maintenance should encourage field epidemiologists and clinical practitioners in endemic countries to secure their own strain collection with the help of the French BRC-Leish.

  2. Including a Service Learning Educational Research Project in a Biology Course-I: Assessing Community Awareness of Childhood Lead Poisoning

    Science.gov (United States)

    Abu-Shakra, Amal; Saliim, Eric

    2012-01-01

    A university course project was developed and implemented in a biology course, focusing on environmental problems, to assess community awareness of childhood lead poisoning. A set of 385 questionnaires was generated and distributed in an urban community in North Carolina, USA. The completed questionnaires were sorted first into yes and no sets…

  3. Recent developments in the Los Alamos National Laboratory Plutonium Facility Waste Tracking System-automated data collection pilot project

    International Nuclear Information System (INIS)

    Martinez, B.; Montoya, A.; Klein, W.

    1999-01-01

    The waste management and environmental compliance group (NMT-7) at the Los Alamos National Laboratory has initiated a pilot project for demonstrating the feasibility and utility of automated data collection as a solution for tracking waste containers at the Los Alamos National Laboratory Plutonium Facility. This project, the Los Alamos Waste Tracking System (LAWTS), tracks waste containers during their lifecycle at the facility. LAWTS is a two-tiered system consisting of a server/workstation database and reporting engine and a hand-held data terminal-based client program for collecting data directly from tracked containers. New containers may be added to the system from either the client unit or from the server database. Once containers are in the system, they can be tracked through one of three primary transactions: Move, Inventory, and Shipment. Because LAWTS is a pilot project, it also serves as a learning experience for all parties involved. This paper will discuss many of the lessons learned in implementing a data collection system in the restricted environment. Specifically, the authors will discuss issues related to working with the PPT 4640 terminal system as the data collection unit. They will discuss problems with form factor (size, usability, etc.) as well as technical problems with wireless radio frequency functions. They will also discuss complications that arose from outdoor use of the terminal (barcode scanning failures, screen readability problems). The paper will conclude with a series of recommendations for proceeding with LAWTS based on experience to date

  4. Contingency plan for the Old Hydrofracture Facility tanks sluicing project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-10-01

    Lockheed Martin Energy Systems, Inc. (Energy Systems), plans to begin a sluicing (flushing) and pumping project to remove the contents from five inactive, underground storage tanks at the Old Hydrofracture Facility (OHF) at the Oak Ridge National Laboratory (ORNL) in Oak Ridge, Tennessee. The tank contents will be transferred to the Melton Valley Storage Tanks, which are part of the active waste treatment system at ORNL. The purpose of the project is to minimize the risk of leaking the highly radioactive material to the environment. The five OHF tanks each contain a layer of sludge and a layer of supernatant. Based on a sampling project undertaken in 1995, the sludge in the tanks has been characterized as transuranic and mixed waste and the supernatants have been characterized as mixed waste. The combined radioactivity of the contents of the five tanks is approximately 29,500 Ci. This contingency plan is based on the preliminary design for the project and describes a series of potential accident/release scenarios for the project. It outlines Energy Systems' preliminary plans for prevention, detection, and mitigation. Prevention/detection methods range from using doubly contained pipelines to alarmed sensors and automatic pump cutoff systems. Plans for mitigation range from pumping leaked fluids from the built-in tank drainage systems and cleaning up spilled liquids to personnel evacuation

  5. A Western Blot-based Investigation of the Yeast Secretory Pathway Designed for an Intermediate-Level Undergraduate Cell Biology Laboratory

    Science.gov (United States)

    Hood-DeGrenier, Jennifer K.

    2008-01-01

    The movement of newly synthesized proteins through the endomembrane system of eukaryotic cells, often referred to generally as the secretory pathway, is a topic covered in most intermediate-level undergraduate cell biology courses. An article previously published in this journal described a laboratory exercise in which yeast mutants defective in…

  6. Actin Immobilization on Chitin for Purifying Myosin II: A Laboratory Exercise That Integrates Concepts of Molecular Cell Biology and Protein Chemistry

    Science.gov (United States)

    de Souza, Marcelle Gomes; Grossi, Andre Luiz; Pereira, Elisangela Lima Bastos; da Cruz, Carolina Oliveira; Mendes, Fernanda Machado; Cameron, Luiz Claudio; Paiva, Carmen Lucia Antao

    2008-01-01

    This article presents our experience on teaching biochemical sciences through an innovative approach that integrates concepts of molecular cell biology and protein chemistry. This original laboratory exercise is based on the preparation of an affinity chromatography column containing F-actin molecules immobilized on chitin particles for purifying…

  7. Status report on the Advanced Photon Source Project at Argonne National Laboratory

    International Nuclear Information System (INIS)

    Huebner, R.H. Sr.

    1989-01-01

    The Advanced Photon Source at Argonne National Laboratory is designed as a national synchrotron radiation user facility which will provide extremely bright, highly energetic x-rays for multidisciplinary research. When operational, the Advanced Photon Source will accelerate positrons to a nominal energy of 7 GeV. The positrons will be manipulated by insertion devices to produce x-rays 10,000 times brighter than any currently available for research. Accelerator components, insertion devices, optical elements, and optical-element cooling schemes have been and continue to be the subjects of intensive research and development. A call for Letters of Intent from prospective users of the Advanced Photon Source has resulted in a substantial response from industrial, university, and national laboratory researchers

  8. Strengthening of an advanced automated radiation laboratory. Hungary. Terminal report project findings and recommendations

    International Nuclear Information System (INIS)

    1992-01-01

    An Advanced Automated Radiation Laboratory was completed; the hardware and software bases are now suitable for up-to-date kinetical measurements in radiation chemistry and technology both for Hungarian experts and for specialists from abroad. It would be possible and useful e.g. to organize further training courses in the field of radiation chemistry and technology by the IAEA and/or the UNDP, as well as to send fellows from developing countries for practical research work or other purposes (TCDC)

  9. Independent technical review of the Sandia National Laboratories Environmental Restoration Project

    International Nuclear Information System (INIS)

    1994-11-01

    An Independent Technical Review was conducted of the Environmental Restoration Project. The objective of the review was recommendations, from a commercial perspective, on a systems level path forward to safe, minimum cost and schedule project completion. The work presented represents the consensus analysis and recommendations of thirteen individuals with varied backgrounds, expertise, and experience. The ITR team recommends that the barriers to the opportunity described in the diagnosis be eliminated using an integrated DOE-Sandia system approach. Piecemeal changes will not result in the desired commercial efficiency. DOE needs to operate as the contracting agency for a Major System Acquisition. If it does not, commercial performance will not be achieved regardless of the contractor. Likewise, Sandia needs to establish and implement the necessary project structure and management systems to operate with commercial contractor like efficiency

  10. Laboratory-based respiratory virus surveillance pilot project on select cruise ships in Alaska, 2013-15.

    Science.gov (United States)

    Rogers, Kimberly B; Roohi, Shahrokh; Uyeki, Timothy M; Montgomery, David; Parker, Jayme; Fowler, Nisha H; Xu, Xiyan; Ingram, Deandra J; Fearey, Donna; Williams, Steve M; Tarling, Grant; Brown, Clive M; Cohen, Nicole J

    2017-09-01

    Influenza outbreaks can occur among passengers and crews during the Alaska summertime cruise season. Ill travellers represent a potential source for introduction of novel or antigenically drifted influenza virus strains to the United States. From May to September 2013-2015, the Alaska Division of Public Health, the Centers for Disease Control and Prevention (CDC), and two cruise lines implemented a laboratory-based public health surveillance project to detect influenza and other respiratory viruses among ill crew members and passengers on select cruise ships in Alaska. Cruise ship medical staff collected 2-3 nasopharyngeal swab specimens per week from passengers and crew members presenting to the ship infirmary with acute respiratory illness (ARI). Specimens were tested for respiratory viruses at the Alaska State Virology Laboratory (ASVL); a subset of specimens positive for influenza virus were sent to CDC for further antigenic characterization. Of 410 nasopharyngeal specimens, 83% tested positive for at least one respiratory virus; 71% tested positive for influenza A or B virus. Antigenic characterization of pilot project specimens identified strains matching predominant circulating seasonal influenza virus strains, which were included in the northern or southern hemisphere influenza vaccines during those years. Results were relatively consistent across age groups, recent travel history, and influenza vaccination status. Onset dates of illness relative to date of boarding differed between northbound (occurring later in the voyage) and southbound (occurring within the first days of the voyage) cruises. The high yield of positive results indicated that influenza was common among passengers and crews sampled with ARI. This finding reinforces the need to bolster influenza prevention and control activities on cruise ships. Laboratory-based influenza surveillance on cruise ships may augment inland influenza surveillance and inform control activities. However, these

  11. Solid low-level radioactive waste volume projections at Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Art, K.; Minton-Hughes, J.; Peper, C.

    1995-01-01

    In response to regulatory requirements, the current economic environment, and diminishing on-site low-level radioactive waste (LLW) disposal capacity, LANL needed to develop a system to collect data on future LLW generation that would comply with DOE Order 5820. 2A and be an effective facility planning tool. The LANL Volume Projections Project (VPP) was created to meet these needs. This paper describes objectives, scope, and components of the VPP that will provide information essential to future facility planning and development

  12. Bure's underground research laboratory: general framework, objectives, siting process and schedule of the URL project

    International Nuclear Information System (INIS)

    Gaussen, J.L.

    2001-01-01

    Bure URL project is one of the components of the French research program dedicated to the study of HLLLW (High Level Long Lived Radioactive Waste) disposal in geologic repository within the framework of the 1991 Radioactive Waste Act. Pursuant to the said act, the objective of the URL project is to participate in the ''evaluation of options for retrievable or non- retrievable disposal in deep geologic formations''. More precisely, the goal of this URL, which is situated 300 km East of Paris, is to gain a better knowledge of a site capable of hosting a geologic repository. (author)

  13. Consensus implementation of a groundwater remediation project at the Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    Hastings, K.R.; Carlson, D.S.

    1996-01-01

    Because of significant characterization uncertainties existing when the Record of Decision was signed and the unfavorable national reputation of groundwater pump and treat remediation projects, the Test Area North (TAN) groundwater ROD includes the evaluation of five emerging technologies that show potential for treating the organic contamination in situ or reducing the toxicity of contaminants above ground. Treatability studies will be conducted to ascertain whether any may be suitable for implementation at TAN to yield more timely or cost effective restoration of the aquifer. The implementation approach established for the TAN groundwater project is a consensus approach, maximizing a partnership relation with stakeholders in constant, iterative implementation decision making

  14. Cold test plan for the Old Hydrofracture Facility tank contents removal project, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1997-11-01

    This Old Hydrofracture Facility (OHF) Tanks Contents Removal Project Cold Test Plan describes the activities to be conducted during the cold test of the OHF sluicing and pumping system at the Tank Technology Cold Test Facility (TTCTF). The TTCTF is located at the Robotics and Process Systems Complex at the Oak Ridge National Laboratory (ORNL). The cold test will demonstrate performance of the pumping and sluicing system, fine-tune operating instructions, and train the personnel in the actual work to be performed. After completion of the cold test a Technical Memorandum will be prepared documenting completion of the cold test, and the equipment will be relocated to the OHF site

  15. 75 FR 55109 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2010-09-09

    .... Satisfaction of customers; and 7. Workforce satisfaction with the personnel management system. An evaluation... personnel system under an appropriate demonstration project as defined in section 342(b) of Public Law 103... for the TARDEC mission, adjust the workforce for change, and improve workforce satisfaction. The...

  16. A Computer-Based Laboratory Project for the Study of Stimulus Generalization and Peak Shift

    Science.gov (United States)

    Derenne, Adam; Loshek, Eevett

    2009-01-01

    This paper describes materials designed for classroom projects on stimulus generalization and peak shift. A computer program (originally written in QuickBASIC) is used for data collection and a Microsoft Excel file with macros organizes the raw data on a spreadsheet and creates generalization gradients. The program is designed for use with human…

  17. 78 FR 34655 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

    Science.gov (United States)

    2013-06-10

    ... CONTACT: Office of Naval Research: Ms. Margaret J. Mitchell, Director, Human Resources Office, Office of... established position management criteria, and (2) Pay Band V of the S&E Professional Career Track require... Personnel Management Demonstration Project, Department of Navy, Office of Naval Research (ONR); Proposed...

  18. Vacuum system of Synchrotron Light National Laboratory (LNLS) linear accelerator: project and results

    International Nuclear Information System (INIS)

    Rocha, L.R.B.; Gomes, P.A.P.

    1990-01-01

    The LINAC, electron linear accelerator used as injector for storage ring in production of synchrotron radiation at Laboratorio Nacional de Luz Sincrotron (LNLS) is described. The LINAC basic parameters, the dimension calculations of vacuum system and the final project with all components are presented. The vacuum system for electron gun tests and accelerator structure tests is described. (M.C.K.)

  19. A Tire Gasification Senior Design Project That Integrates Laboratory Experiments and Computer Simulation

    Science.gov (United States)

    Weiss, Brian; Castaldi, Marco J.

    2006-01-01

    A reactor to convert waste rubber tires to useful products such as CO and H2, was investigated in a university undergraduate design project. The student worked individually with mentorship from a faculty professor who aided the student with professional critique. The student was able to research the background of the field and conceive of a novel…

  20. [Establishment of Quality Control System of Nucleic Acid Detection for Ebola Virus in Sierra Leone-China Friendship Biological Safety Laboratory].

    Science.gov (United States)

    Wang, Qin; Zhang, Yong; Nie, Kai; Wang, Huanyu; Du, Haijun; Song, Jingdong; Xiao, Kang; Lei, Wenwen; Guo, Jianqiang; Wei, Hejiang; Cai, Kun; Wang, Yanhai; Wu, Jiang; Gerald, Bangura; Kamara, Idrissa Laybohr; Liang, Mifang; Wu, Guizhen; Dong, Xiaoping

    2016-03-01

    The quality control process throughout the Ebola virus nucleic acid detection in Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab) was described in detail, in order to comprehensively display the scientific, rigorous, accurate and efficient practice in detection of Ebola virus of first batch detection team in SLE-CHN Biosafety Lab. Firstly, the key points of laboratory quality control system was described, including the managements and organizing, quality control documents and information management, instrument, reagents and supplies, assessment, facilities design and space allocation, laboratory maintenance and biosecurity. Secondly, the application of quality control methods in the whole process of the Ebola virus detection, including before the test, during the test and after the test, was analyzed. The excellent and professional laboratory staffs, the implementation of humanized management are the cornerstone of the success; High-level biological safety protection is the premise for effective quality control and completion of Ebola virus detection tasks. And professional logistics is prerequisite for launching the laboratory diagnosis of Ebola virus. The establishment and running of SLE-CHN Biosafety Lab has landmark significance for the friendship between Sierra Leone and China, and the lab becomes the most important base for Ebola virus laboratory testing in Sierra Leone.

  1. Effect of residual H2O2 from advanced oxidation processes on subsequent biological water treatment: A laboratory batch study.

    Science.gov (United States)

    Wang, Feifei; van Halem, Doris; Liu, Gang; Lekkerkerker-Teunissen, Karin; van der Hoek, Jan Peter

    2017-10-01

    H 2 O 2 residuals from advanced oxidation processes (AOPs) may have critical impacts on the microbial ecology and performance of subsequent biological treatment processes, but little is known. The objective of this study was to evaluate how H 2 O 2 residuals influence sand systems with an emphasis on dissolved organic carbon (DOC) removal, microbial activity change and bacterial community evolution. The results from laboratory batch studies showed that 0.25 mg/L H 2 O 2 lowered DOC removal by 10% while higher H 2 O 2 concentrations at 3 and 5 mg/L promoted DOC removal by 8% and 28%. A H 2 O 2 dosage of 0.25 mg/L did not impact microbial activity (as measured by ATP) while high H 2 O 2 dosages, 1, 3 and 5 mg/L, resulted in reduced microbial activity of 23%, 37% and 37% respectively. Therefore, DOC removal was promoted by the increase of H 2 O 2 dosage while microbial activity was reduced. The pyrosequencing results illustrated that bacterial communities were dominated by Proteobacteria. The presence of H 2 O 2 showed clear influence on the diversity and composition of bacterial communities, which became more diverse under 0.25 mg/L H 2 O 2 but conversely less diverse when the dosage increased to 5 mg/L H 2 O 2 . Anaerobic bacteria were found to be most sensitive to H 2 O 2 as their growth in batch reactors was limited by both 0.25 and 5 mg/L H 2 O 2 (17-88% reduction). In conclusion, special attention should be given to effects of AOPs residuals on microbial ecology before introducing AOPs as a pre-treatment to biological (sand) processes. Additionally, the guideline on the maximum allowable H 2 O 2 concentration should be properly evaluated. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  2. Practical development of laboratory of biologics technology of the Palladin Institute of Biochemistry of NAS of Ukraine for the period 1991-2010

    Directory of Open Access Journals (Sweden)

    G. G. Lugovska

    2015-10-01

    Full Text Available The paper presents the results of inventive activity of the Laboratory of Biologics Technology of the Palladin Institute of Biochemistry, NAS of Ukraine, under the leadership of Z.M.Datsenko, Cand.Sc. (Biol., during 1991-2010. The laboratory researchers have developed new technologies for production of medicines of lipid-protein nature, which are based on endogenous complexes of biologically active substances included in the composition of biomembranes of different origin. The researchers have created new technologies for drug PANTOCRINE (for injections and oral administration from antlers of deers and horns of farm animals that have significantly higher biological activity compared with the commercial drug. They have also created new biologically active drugs from antlers with a specific action: HIPPOCAMP, reducing blood pressure in various forms of hypertension, and PANTERON – biological regulator of synthesis of steroid hormones. Further the researchers have developed technologies for obtaining biologically active complexes of various specific action from marine organisms (calamari, clams mussels, shellfish: CALMOFIL and MOLUFIL, therapeutic agents for replacement therapy of surfactant system of the lungs; FILOMEK, the agent for prophylaxis and treatment of a human reproductive system disorders; MOLUSTERON, the glicolipopeptide complex, three individual physiologically active compositions being differentially obtained from the latter: lipopeptide composition – for treatment of hypertension, nucleopeptide one – for treatment of hormonal disorders, and phospholipid – for treatment of lung diseases under the surfactant shortage.

  3. Elementary and Advanced Computer Projects for the Physics Classroom and Laboratory

    Science.gov (United States)

    1992-12-01

    the language of science and engineering in industry and government laboratories (alth..4h C is becoming a powerful competitor ). RM/FORTRAN (cost $400...an AD m1ber may be obtained from the National Technical Informatio Service, U.S. Departmen of Commwce, Spng Virgin 22151. Other pipes ar available from...pp.. Nov 1989 "-2- PP 471 PP 499 Holiday . Mary Robin. Methodology of an Event-Driven Siegel. Adam B., A Brave New Curriculum for a Brave Monte Carlo

  4. Groundwater flow modeling in construction phase of the Mizunami Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Onoe, Hironori; Saegusa, Hiromitsu; Takeuchi, Ryuji

    2016-01-01

    This paper comprehensively describes the result of groundwater flow modeling using data of hydraulic responses due to construction of Mizunami Underground Research Laboratory (MIU) in Mizunami, Gifu, in order to update hydrogeological model based on stepwise approach for crystalline fractured rock in Japan. The results showed that large scale hydraulic compartment structures which has significant influence on change of groundwater flow characteristics are distributed around MIU. Furthermore, it is concluded that hydrogeological monitoring data and groundwater flow modeling during construction of deep underground facilities are effective for hydrogeological characterization of heterogeneous fractured rock. (author)

  5. The Spanish biology/disease initiative within the human proteome project: Application to rheumatic diseases.

    Science.gov (United States)

    Ruiz-Romero, Cristina; Calamia, Valentina; Albar, Juan Pablo; Casal, José Ignacio; Corrales, Fernando J; Fernández-Puente, Patricia; Gil, Concha; Mateos, Jesús; Vivanco, Fernando; Blanco, Francisco J

    2015-09-08

    The Spanish Chromosome 16 consortium is integrated in the global initiative Human Proteome Project, which aims to develop an entire map of the proteins encoded following a gene-centric strategy (C-HPP) in order to make progress in the understanding of human biology in health and disease (B/D-HPP). Chromosome 16 contains many genes encoding proteins involved in the development of a broad range of diseases, which have a significant impact on the health care system. The Spanish HPP consortium has developed a B/D platform with five programs focused on selected medical areas: cancer, obesity, cardiovascular, infectious and rheumatic diseases. Each of these areas has a clinical leader associated to a proteomic investigator with the responsibility to get a comprehensive understanding of the proteins encoded by Chromosome 16 genes. Proteomics strategies have enabled great advances in the area of rheumatic diseases, particularly in osteoarthritis, with studies performed on joint cells, tissues and fluids. In this manuscript we describe how the Spanish HPP-16 consortium has developed a B/D platform with five programs focused on selected medical areas: cancer, obesity, cardiovascular, infectious and rheumatic diseases. Each of these areas has a clinical leader associated to a proteomic investigator with the responsibility to get a comprehensive understanding of the proteins encoded by Chromosome 16 genes. We show how the Proteomic strategy has enabled great advances in the area of rheumatic diseases, particularly in osteoarthritis, with studies performed on joint cells, tissues and fluids. This article is part of a Special Issue entitled: HUPO 2014. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Life cycle baseline summary for ADS 6504IS Isotopes Facilities Deactivation Project at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-11-01

    The purpose of the Isotopes Facility Deactivation Project (IFDP) is to place former isotopes production facilities at the Oak Ridge National Laboratory in a safe, stable, and environmentally sound condition; suitable for an extended period of minimum surveillance and maintenance (S ampersand M) and as quickly and economically as possible. This baseline plan establishes the official target schedule for completing the deactivation work and the associated budget required for deactivation and the necessary S ampersand M. Deactivation of the facilities 3026C, 3026D, 3028, 3029, 3038E, 3038M, and 3038AHF, the Center Circle buildings 3047, 3517, and 7025 will continue though Fiscal Year (FY) 1999. The focus of the project in the early years will be on the smaller buildings that require less deactivation and can bring an early return in reducing S ampersand M costs. This baseline plan covers the period from FY1995 throughout FY2000. Deactivation will continue in various facilities through FY1999. A final year of S ampersand M will conclude the project in FY2000. The estimated total cost of the project during this period is $51M

  7. The high current, fast, 100ns, Linear Transformer Driver (LTD) developmental project at Sandia National Laboratories

    International Nuclear Information System (INIS)

    Ward, Kevin S.; Long, Finis W.; Sinebryukhov, Vadim A.; Kim, Alexandre A.; Wakeland, Peter Eric; McKee, G. Randall; Woodworth, Joseph Ray; McDaniel, Dillon Heirman; Fowler, William E.; Mazarakis, Michael Gerrassimos; Porter, John Larry Jr.; Struve, Kenneth William; Stygar, William A.; LeChien, Keith R.; Matzen, Maurice Keith

    2010-01-01

    Sandia National Laboratories, Albuquerque, N.M., USA, in collaboration with the High Current Electronic Institute (HCEI), Tomsk, Russia, is developing a new paradigm in pulsed power technology: the Linear Transformer Driver (LTD) technology. This technological approach can provide very compact devices that can deliver very fast high current and high voltage pulses straight out of the cavity with out any complicated pulse forming and pulse compression network. Through multistage inductively insulated voltage adders, the output pulse, increased in voltage amplitude, can be applied directly to the load. The load may be a vacuum electron diode, a z-pinch wire array, a gas puff, a liner, an isentropic compression load (ICE) to study material behavior under very high magnetic fields, or a fusion energy (IFE) target. This is because the output pulse rise time and width can be easily tailored to the specific application needs. In this paper we briefly summarize the developmental work done in Sandia and HCEI during the last few years, and describe our new MYKONOS Sandia High Current LTD Laboratory.

  8. Measurement plan and observational construction program on drift excavation at the Horonobe Underground Research Laboratory project

    International Nuclear Information System (INIS)

    Yamasaki, Masanao; Yamaguchi, Takehiro; Funaki, Hironori; Fujikawa, Daisuke; Tsusaka, Kimikazu

    2008-09-01

    The Horonobe URL Project is being pursued by the Japan Atomic Energy Agency (JAEA) to enhance the reliability of relevant disposal technologies through investigations of the deep geological environment within the host sedimentary formations at Horonobe, northern Hokkaido. The project consists of two major research areas, 'Geoscientific Research' and 'R and D' on Geological Disposal', and proceeds in three overlapping phases, 'Phase I: Surface-based investigation', 'Phase II: Construction' and 'Phase III: Operation', over a period of 20 years. On the Horonobe URL Project, 'Phase 1' was finished in 2005FY and construction of the underground facility was started since then. Now, 'Phase 2' (investigations during construction of the underground facilities) is on-going. On the 'Development of engineering techniques for use in the deep underground environment' in Phase 1, based on the various types of data acquired on investigations from the surface, the design of underground facility in advance was planned. At the inception of the Phase II investigations, an investigation report titled 'Measurement Plan and Observational Construction Program on Shaft Excavation at the Horonobe URL Project' (hereinafter referred to as 'Observational Construction Program') was published. The Observational Construction Program summarizes followings from the Phase I investigations: measurements for safety/reasonable construction, measurements for R and D on enhancement of shaft design/construction technology, and measurements for verification of the deep geological environment model estimated before shaft excavation, and it is on-going. This report summarizes the measurement plan during construction of drifts based on the design in advance and the observational construction program for feedback measurements data into design and construction on subsequent steps. This report also describes about design and construction management program of underground facility and R and D program on

  9. Horonobe Underground Research Laboratory project. Current status on the surface-based investigation

    International Nuclear Information System (INIS)

    Hama, Katsuhiro; Ishii, Eiichi

    2004-01-01

    Aims of the Horonobe URL project are presenting concrete geological environment as an example of sedimentary formation and confirming reliability of technologies for geological disposal of High-Level Radioactive Waste (HLW) by applying them to actual geological condition of sedimentary formation. Social aim is providing opportunities for general public to experience the actual deep underground circumstance and R and D activities to be conducted there. (author)

  10. The RACER (risk analysis, communication, evaluation, and reduction) stakeholder environmental data transparency project for Los Alamos National Laboratory

    International Nuclear Information System (INIS)

    Echohawk, John Chris; Dorries, Alison M.; Eberhart, Craig F.; Werdel, Nancy

    2008-01-01

    The RACER (Risk Analysis, Communication, Evaluation, and Reduction) project was created in 2003, as an effort to enhance the Los Alamos National Laboratory's ability to effectively communicate the data and processes used to evaluate environmental risks to the public and the environment. The RACER project staff consists of members of Risk Assessment Corporation, Los Alamos National Laboratory (LANL), and the New Mexico Environment Department (NMED). RACER staff worked closely with members of the community, tribal governments, and others within NMED and LANL to create innovative tools and a process that could provide information to regulators, LANL and the community about the sources of public health risk and ecological impact from LAN L operations. The RACER Data Analysis Tool (DA T) provides the public with webbased access to environmental measurement data collected in and around the LANL site. Its purpose is to provide a 'transparent' view to the public of all data collected by LANL and NMED regarding the LANL site. The DAT is available to the public at 'www.racernm.com'.

  11. Oppenheimer's Box of Chocolates: Remediation of the Manhattan Project Landfill at Los Alamos National Laboratory - 12283

    Energy Technology Data Exchange (ETDEWEB)

    Allen, Donald L.; Ramsey, Susan S.; Finn, Kevin P.; Chaloupka, Allan B. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)

    2012-07-01

    Material Disposal Area B (MDA B) is the oldest radioactive waste disposal facility at Los Alamos National Laboratory. Operated from 1944-48, MDA B was the disposal facility for the Manhattan Project. Recognized as one of the most challenging environmental remediation projects at Los Alamos, the excavation of MDA B received $110 million from the American Recovery and Reinvestment Act of 2009 to accelerate this complex remediation work. Several factors combined to create significant challenges to remediating the landfill known in the 1940's as the 'contaminated dump'. The secrecy surrounding the Manhattan Project meant that no records were kept of radiological materials and chemicals disposed or of the landfill design. An extensive review of historical documents and interviews with early laboratory personnel resulted in a list of hundreds of hazardous chemicals that could have been buried in MDA B. Also, historical reports of MDA B spontaneously combusting on three occasions -with 50-foot flames and pink smoke spewing across the mesa during the last incident in 1948-indicated that hazardous materials were likely present in MDA B. To complicate matters further, though MDA B was located on an isolated mesa in the 1940's, the landfill has since been surrounded by a Los Alamos commercial district. The local newspaper, hardware store and a number of other businesses are located directly across the street from MDA B. This close proximity to the public and the potential for hazardous materials in MDA B necessitated conducting remediation work within protective enclosures. Potential chemical hazards and radiological inventory were better defined using a minimally intrusive sampling method called direct push technology (DPT) prior to excavation. Even with extensive sampling and planning the project team encountered many surprises and challenges during the project. The one area where planning did not fail to meet reality was safety. There were no serious

  12. A Project-Based Biochemistry Laboratory Promoting the Understanding and Uses of Fluorescence Spectroscopy in the Study of Biomolecular Structures and Interactions

    Science.gov (United States)

    Briese, Nicholas; Jakubowsk, Henry V.

    2007-01-01

    A laboratory project for a first semester biochemistry course is described, which integrates the traditional classroom study of the structure and function of biomolecules with the laboratory study of these molecules using fluorescence spectroscopy. Students are assigned a specific question addressing the stability/function of lipids, proteins, or…

  13. Rapid, Sensitive, Enzyme-Immunodotting Assay for Detecting Cow Milk Adulteration in Sheep Milk: A Modern Laboratory Project

    Science.gov (United States)

    Inda, Luis A.; Razquín, Pedro; Lampreave, Fermín; Alava, María A.; Calvo, Miguel

    1998-12-01

    Specificity, sensitivity, and experimental simplicity make the immunoenzymatic assay suitable for a variety of laboratories dedicated to diverse activities such as research, quality control in food analysis, or clinical biochemistry. In these assays, the antibody that specifically recognizes the antigen is covalently attached to an enzyme. Once the antigen-antibody immunocomplex is formed, the enzymatic reaction gives a colored product that allows the detection of the initial antigen. The aim of this work was the design of a new laboratory project appropriate for use in courses of biochemistry, immunochemistry, or analytical chemistry. The assay described here detects the presence of cow milk in milk of other species. The main application is the detection of cow milk in sheep milk and cheese. Specific proteins, immunoglobulins (IgG) of the fraudulent bovine milk, are specifically recognized and retained by antibodies immobilized on a membrane. The binding of a second antibody covalently attached to horseradish peroxidase (HRP) allows the development of a visible signal. Thus, students can rapidly detect milk adulterations using a specific, sensitive, and safe experimental approach. The experiment allows students to apply their theoretical knowledge, resulting in a stimulating experience of solving a real problem during a 4-hour laboratory period.

  14. Measurements of copper corrosion in the LOT Project at the Aespoe Hard Rock Laboratory

    International Nuclear Information System (INIS)

    Rosborg, B.; Karnland, O.; Quirk, G.; Werme, L.

    2003-01-01

    Real-time monitoring, of corrosion by means of electrochemical noise and other electrochemical techniques may offer interesting possibilities to estimate the kind and degree of corrosion in a sample or component, and further visualize the corrosion resistance of pure copper in repository environments. As a pilot effort, three cylindrical copper electrodes for such measurements, each of about 100 cm 2 surface area, have been installed in a test parcel in the Aespoe Hard Rock Laboratory and electrochemical measurements using InterCorr's SmartCET system were initiated in May 2001. The first results from real-time monitoring of copper corrosion in the Aespoe HRL under actual repository environment conditions by means of linear polarisation resistance, harmonic distortion analysis and electrochemical noise techniques are presented, and compared with the results obtained from one of the retrieved test parcels. (authors)

  15. Melton Valley Storage Tanks Capacity Increase Project, Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-04-01

    The US Department of Energy (DOE) proposes to construct and maintain additional storage capacity at Oak Ridge National Laboratory (ORNL), Oak Ridge, Tennessee, for liquid low-level radioactive waste (LLLW). New capacity would be provided by a facility partitioned into six individual tank vaults containing one 100,000 gallon LLLW storage tank each. The storage tanks would be located within the existing Melton Valley Storage Tank (MVST) facility. This action would require the extension of a potable water line approximately one mile from the High Flux Isotope Reactor (HFIR) area to the proposed site to provide the necessary potable water for the facility including fire protection. Alternatives considered include no-action, cease generation, storage at other ORR storage facilities, source treatment, pretreatment, and storage at other DOE facilities

  16. Los Alamos National Laboratory Yucca Mountain Site Characterization Project 1992 quality program status report

    International Nuclear Information System (INIS)

    Bolivar, S.L.; Burningham, A.; Chavez, P.

    1994-03-01

    This status report summarizes the activities and accomplishments of the Los Alamos Yucca Mountain Site Characterization Project's quality assurance program for calendar year 1992. The report includes major sections on Program Activities and Trend Analysis. Program Activities are discussed periodically at quality meetings. The most significant issue addressed in 1992 has been the timely revision of quality administrative procedures. The procedure revision process was streamlined from 55 steps to 7. The number of forms in procedures was reduced by 38%, and the text reduced by 29%. This allowed revision in 1992 of almost half of all implementing procedures. The time necessary to complete the revision process (for a procedure) was reduced from 11 months to 3 months. Other accomplishments include the relaxation of unnecessarily strict training requirements, requiring quality assurance reviews only from affected organizations, and in general simplifying work processes. All members of the YMP received training to the new Orientation class Eleven other training classed were held. Investigators submitted 971 records to the Project and only 37 were rejected. The software program has 115 programs approved for quality-affecting work. The Project Office conducted 3 audits and 1 survey of Los Alamos activities. We conducted 14 audits and 4 surveys. Eight corrective action reports were closed, leaving only one open. Internally, 22 deficiencies were recognized. This is a decrease from 65 in 1991. Since each deficiency requires about 2 man weeks to resolve, the savings are significant. Problems with writing acceptable deficiency reports have essentially disappeared. Trend reports for 1992 were examined and are summarized herein. Three adverse trends have been closed; one remaining adverse trend will be closed when the affected procedures are revised. The number of deficiencies issued to Los Alamos compared to other participants is minimal

  17. Student evaluation of research projects in a first-year physics laboratory

    International Nuclear Information System (INIS)

    Sharma, Manjula D; Mendez, Alberto; Sefton, Ian M; Khachan, Joe

    2014-01-01

    We describe the evaluation by students of a scheme of open-ended, research-based group project work which has become a standard component of first-year physics courses at the University of Sydney and is now in its 19th year of operation. Data were gathered from two sources: direct observations of the classes and a written survey. A summary of the classroom observations and the results from a detailed analysis of the survey responses are presented. The feedback from the cohort of approximately 800 students is largely positive but we identify a few discrepancies between stated course goals and the results from the survey. (paper)

  18. Los Alamos National Laboratory Yucca Mountain Project Publications (1979-1996)

    International Nuclear Information System (INIS)

    Ruhala, E.R.; Klein, S.H.

    1997-06-01

    This over-350 title publication list reflects the accomplishments of Los Alamos Yucca Mountain Site Characterization Project researchers, who, since 1979, have been conducting multidisciplinary research to help determine if Yucca Mountain, Nevada, is a suitable site for a high-level waste repository. The titles can be accessed in two ways: by year, beginning with 1996 and working back to 1979, and by subject area: mineralogy/petrology/geology, volcanism, radionuclide solubility/ground-water chemistry; radionuclide sorption and transport; modeling/validation/field studies; summary/status reports, and quality assurance

  19. Los Alamos National Laboratory Yucca Mountain Project publications (1979--1994)

    International Nuclear Information System (INIS)

    Bowker, L.M.; Espinosa, M.L.; Klein, S.H.

    1995-11-01

    This over-300 title publication list reflects the accomplishments of Los Alamos Yucca Mountain Site Characterization Project researchers, who, since 1979, have been conducting multidisciplinary research to help determine if Yucca Mountain, Nevada, is a suitable site for a high-level waste repository. The titles can be accessed in two ways: by year, beginning with 1994 and working back to 1979, and by subject area: mineralogy/petrology/geology, volcanism, radionuclide solubility/groundwater chemistry; radionuclide sorption and transport; modeling/validation/field studies; summary/status reports, and quality assurance

  20. Fluorescent biological aerosol particles measured with the Waveband Integrated Bioaerosol Sensor WIBS-4: laboratory tests combined with a one year field study

    Directory of Open Access Journals (Sweden)

    E. Toprak

    2013-01-01

    Full Text Available In this paper bioaerosol measurements conducted with the Waveband Integrated Bioaerosol Sensor mark 4 (WIBS-4 are presented. The measurements comprise aerosol chamber characterization experiments and a one-year ambient measurement period at a semi-rural site in South Western Germany. This study aims to investigate the sensitivity of WIBS-4 to biological and non-biological aerosols and detection of biological particles in the ambient aerosol. Several types of biological and non-biological aerosol samples, including fungal spores, bacteria, mineral dust, ammonium sulphate, combustion soot, and fluorescent polystyrene spheres, were analyzed by WIBS-4 in the laboratory. The results confirm the sensitivity of the ultraviolet light-induced fluorescence (UV-LIF method to biological fluorophores and show the good discrimination capabilities of the two excitation wavelengths/detection wavebands method applied in WIBS-4. However, a weak cross-sensitivity to non-biological fluorescent interferers remains and is discussed in this paper.

    All the laboratory studies have been undertaken in order to prepare WIBS-4 for ambient aerosol measurements. According to the one-year ambient aerosol study, number concentration of fluorescent biological aerosol particles (FBAP show strong seasonal and diurnal variability. The highest number concentration of FBAP was measured during the summer term and decreased towards the winter period when colder and drier conditions prevail. Diurnal FBAP concentrations start to increase after sunset and reach maximum values during the late night and early morning hours. On the other hand, the total aerosol number concentration was almost always higher during daytime than during nighttime and a sharp decrease after sunset was observed. There was no correlation observed between the FBAP concentration and the meteorological parameters temperature, precipitation, wind direction and wind speed. However, a clear correlation was