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Sample records for bioindication hibiscus syriacus

  1. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example

    Energy Technology Data Exchange (ETDEWEB)

    Paoletti, Elena [Institut Plant Protection (IPP), National Council Research (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence (Italy)], E-mail: e.paoletti@ipp.cnr.it; Ferrara, Anna Maria [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Calatayud, Vicent; Cervero, Julia [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Giannetti, Fabio [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Sanz, Maria Jose [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Manning, William J. [Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA 01003-9320 (United States)

    2009-03-15

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300 ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30 ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation. - An Italian population of the deciduous shrub Hibiscus syriacus, a common ornamental species in temperate zones, is recommended as ozone bioindicator.

  2. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example

    International Nuclear Information System (INIS)

    Paoletti, Elena; Ferrara, Anna Maria; Calatayud, Vicent; Cervero, Julia; Giannetti, Fabio; Sanz, Maria Jose; Manning, William J.

    2009-01-01

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300 ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30 ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation. - An Italian population of the deciduous shrub Hibiscus syriacus, a common ornamental species in temperate zones, is recommended as ozone bioindicator

  3. Studies on mutant breeding of Hibiscus syriacus

    Energy Technology Data Exchange (ETDEWEB)

    Song, Hi Sup; Kim, Jin Kyu; Lee, Ki Un; Kim, Young Taik

    1997-01-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has such a characteristic of self-incompatibility that all the plant exist as natural hybrids and have heterogeneous genes. Many domestic 91 varieties of Hibiscus syriacus were collected. Radiosensitivity of H. Syriacus irradiated with {gamma}-ray was investigated in plant cuttings. The plant height was reduced by 45% in 5KR irradiated group, compared to control group. The radiation dose of 5KR could be recommended for mutation breeding of Hibiscus cuttings. Radiosensitivity of {gamma}-ray irradiated Hibiscus seed were investigated. The germination rate, survival rate and plant height was better in the 4KR irradiation plot than control. The radiation dose of 10{approx}12KR are recommended for mutation breeding of Hibiscus. Promising mutant lines were selected form the varieties of Hwarang, Wolsan no. 176, Ilpyondansim, Emille, Hanol, Yongkwang, Saeyongkwang, Chungmu, Imjinhong, Arang, Hungdansim-1 and Hongdansim-2. (author). 66 refs., 16 tabs., 13 figs.

  4. Studies on mutant breeding of Hibiscus syriacus

    International Nuclear Information System (INIS)

    Song, Hi Sup; Kim, Jin Kyu; Lee, Ki Un; Kim, Young Taik.

    1997-01-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has such a characteristic of self-incompatibility that all the plant exist as natural hybrids and have heterogeneous genes. Many domestic 91 varieties of Hibiscus syriacus were collected. Radiosensitivity of H. Syriacus irradiated with γ-ray was investigated in plant cuttings. The plant height was reduced by 45% in 5KR irradiated group, compared to control group. The radiation dose of 5KR could be recommended for mutation breeding of Hibiscus cuttings. Radiosensitivity of γ-ray irradiated Hibiscus seed were investigated. The germination rate, survival rate and plant height was better in the 4KR irradiation plot than control. The radiation dose of 10∼12KR are recommended for mutation breeding of Hibiscus. Promising mutant lines were selected form the varieties of Hwarang, Wolsan no. 176, Ilpyondansim, Emille, Hanol, Yongkwang, Saeyongkwang, Chungmu, Imjinhong, Arang, Hungdansim-1 and Hongdansim-2. (author). 66 refs., 16 tabs., 13 figs

  5. Studies on mutation breeding of hibiscus syriacuse

    International Nuclear Information System (INIS)

    Song, Hee Sub; Kim, Jin Kyu; Lee, Ki Un; Lim, Yong Taek

    1995-12-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has ahch a characteristic of self-incompatibility that all the plants exist as natural hybrids and have heterogeneous genes. Thirth two domestic varieties were propagated. Radiosensitivity of H. syriacus irradiated with gamma ray was investigated in plant cuttings. The plant height was reduced by 45 percent in 5 kR irradiated group compared to control group. The radiation dose of 5 kR could be rrecommended for mutation breeding of Hibiscus cuttings. Promising mutant lines were selected form the varieties of Hwarang Wolsan 176, I1pyondansim and Emille. 6 tabs., 2 figs., 13 refs., 4 ills. (Author)

  6. Radiosensitivity and chimera formation in Hibiscus syriacus

    International Nuclear Information System (INIS)

    Kwon, S.H.; Won, J.L.

    1980-01-01

    Radiosensitivity of gamma-irradiated Hibiscus syriacus and chimera formation were investigated. The lethal dose-50 percent with respect to seeding and cuttings was 15kR and 2 approximately 3 kR respectively, chlorophyll mutation rate of seeds irradiated with 15 kR being about 13 percent. The degree of chimeric leaf mutants from the buds by radiation treatment depends on the bud position of the branch. Buds of the middle part of V 1 branch seemed to be more multi-cellular condition than the upper and low part when irradiation was made. It is assumed that at least two primordia of V 2 branch were already differentiated from the V 1 branch in Hibiscus syriacus plant. (Author)

  7. [Molecular identification of Hibiscus syriacus and its adulterants using ITS2 barcode].

    Science.gov (United States)

    Liu, Yi-Mei; Jin, Li-Na; Xiong, Yong-Xin; Wu, Lan; Chen, Ke-Li

    2014-03-01

    To identify Hibiscus syriacus and its adulterants using DNA barcoding technique. Nine samples of five species were PCR amplified and sequenced, and twelve samples were downloaded from the GenBank. The intra-specific and interspecific K2P distances were calculated, and neighbor-joining( NJ) tree was constructed by MEGA 5.0. The results showed the intra-specific genetic distances of Hibiscus syriacus were ranged from 0.009 to 0.056, which were far lower than inter-specific genetic distances between Hibiscus syriacus and its adulterants (0.236 - 0.301). Variable sites within Hibiscus syriacus ranged from 2 to 9 which were far less than the adulterants (45 - 52); Different samples of Hibiscus syriacus were gathered together and could be distinguished from its adulterants by NJ tree. ITS2 can discriminate Hibiscus syriacus from its adulterants correctly. The ITS2 region is an efficient barcode for authentication of Hibiscus syriacus and its adulterants.

  8. Studies on mutation breeding of hibiscus Syriacus

    International Nuclear Information System (INIS)

    Song, Heui Sub; Lee, Ki Woon; Im, Yong Taek

    1994-12-01

    Hibiscus(Hibiscus syracuse L.) has been know as a national flower of Korea science old times. Although there are some ancient records that the Hibiscus had been planted in large quantities in Korea, Japanese had dug out all the good plants of Hibiscus in this country during their colonial period. But Hibiscus has such a characteristics of self-incompatibility that all the plants exist as a hybrid naturally and have heterogeneous genes. Therefore many good characters can be taken out from the surviving plants. Many domestic 78 varieties of Hibiscus syracuse were collected and propagated 26 varieties cuttings. Radiosensitivity of gamma-ray irradiated Hibiscus syracuse were investigated the germination rate, survival rate, plant height was with the increase of 4 kR better than control. The radiation doses of 10-12 kR are recommended for mutation breeding of Hibiscus. 6 figs, 11 tabs, 41 refs. (Author)

  9. Studies on mutation breeding of hibiscus Syriacus

    Energy Technology Data Exchange (ETDEWEB)

    Song, Heui Sub; Lee, Ki Woon; Im, Yong Taek [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1994-12-01

    Hibiscus(Hibiscus syracuse L.) has been know as a national flower of Korea science old times. Although there are some ancient records that the Hibiscus had been planted in large quantities in Korea, Japanese had dug out all the good plants of Hibiscus in this country during their colonial period. But Hibiscus has such a characteristics of self-incompatibility that all the plants exist as a hybrid naturally and have heterogeneous genes. Therefore many good characters can be taken out from the surviving plants. Many domestic 78 varieties of Hibiscus syracuse were collected and propagated 26 varieties cuttings. Radiosensitivity of gamma-ray irradiated Hibiscus syracuse were investigated the germination rate, survival rate, plant height was with the increase of 4 kR better than control. The radiation doses of 10-12 kR are recommended for mutation breeding of Hibiscus. 6 figs, 11 tabs, 41 refs. (Author).

  10. The complete chloroplast genome sequence of Hibiscus syriacus.

    Science.gov (United States)

    Kwon, Hae-Yun; Kim, Joon-Hyeok; Kim, Sea-Hyun; Park, Ji-Min; Lee, Hyoshin

    2016-09-01

    The complete chloroplast genome sequence of Hibiscus syriacus L. is presented in this study. The genome is composed of 161 019 bp in length, with a typical circular structure containing a pair of inverted repeats of 25 745 bp of length separated by a large single-copy region and a small single-copy region of 89 698 bp and 19 831 bp of length, respectively. The overall GC content is 36.8%. One hundred and fourteen genes were annotated, including 81 protein-coding genes, 4 ribosomal RNA genes and 29 transfer RNA genes.

  11. Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes

    OpenAIRE

    Yoon, Seok Won; Lee, Kang Pa; Kim, Do-Yoon; Hwang, Dae Il; Won, Kyung-Jong; Lee, Dae Won; Lee, Hwan Myung

    2017-01-01

    Background: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. Objective: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. Materials and Methods: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysi...

  12. Genome analysis of Hibiscus syriacus provides insights of polyploidization and indeterminate flowering in woody plants.

    Science.gov (United States)

    Kim, Yong-Min; Kim, Seungill; Koo, Namjin; Shin, Ah-Young; Yeom, Seon-In; Seo, Eunyoung; Park, Seong-Jin; Kang, Won-Hee; Kim, Myung-Shin; Park, Jieun; Jang, Insu; Kim, Pan-Gyu; Byeon, Iksu; Kim, Min-Seo; Choi, JinHyuk; Ko, Gunhwan; Hwang, JiHye; Yang, Tae-Jin; Choi, Sang-Bong; Lee, Je Min; Lim, Ki-Byung; Lee, Jungho; Choi, Ik-Young; Park, Beom-Seok; Kwon, Suk-Yoon; Choi, Doil; Kim, Ryan W

    2017-02-01

    Hibiscus syriacus (L.) (rose of Sharon) is one of the most widespread garden shrubs in the world. We report a draft of the H. syriacus genome comprised of a 1.75 Gb assembly that covers 92% of the genome with only 1.7% (33 Mb) gap sequences. Predicted gene modeling detected 87,603 genes, mostly supported by deep RNA sequencing data. To define gene family distribution among relatives of H. syriacus, orthologous gene sets containing 164,660 genes in 21,472 clusters were identified by OrthoMCL analysis of five plant species, including H. syriacus, Arabidopsis thaliana, Gossypium raimondii, Theobroma cacao and Amborella trichopoda. We inferred their evolutionary relationships based on divergence times among Malvaceae plant genes and found that gene families involved in flowering regulation and disease resistance were more highly divergent and expanded in H. syriacus than in its close relatives, G. raimondii (DD) and T. cacao. Clustered gene families and gene collinearity analysis revealed that two recent rounds of whole-genome duplication were followed by diploidization of the H. syriacus genome after speciation. Copy number variation and phylogenetic divergence indicates that WGDs and subsequent diploidization led to unequal duplication and deletion of flowering-related genes in H. syriacus and may affect its unique floral morphology. © The Author 2016. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  13. Sphingomonas rhizophila sp. nov., isolated from rhizosphere of Hibiscus syriacus.

    Science.gov (United States)

    Yan, Zheng-Fei; Lin, Pei; Won, Kyung-Hwa; Li, Chang-Tian; Park, GyungSoo; Chin, ByungSun; Kook, MooChang; Wang, Qi-Jun; Yi, Tae-Hoo

    2018-02-01

    A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61 T ), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61 T were identified as Sphingomonas ginsengisoli KCTC 12630 T (97.9 %), Sphingomonas jaspsi DSM 18422 T (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146 T (97.4 %), Sphingomonassediminicola KCTC 12629 T (97.2 %), 'Sphingomonas swuensis' KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718 T (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1ω7c, C18 : 1ω9t and/or C18 : 1ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61 T was 65.6 mol%. The DNA-DNA relatedness values between strain THG-T61 T and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61 T (=KACC 19189 T =CCTCC AB 2016245 T ).

  14. Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes.

    Science.gov (United States)

    Yoon, Seok Won; Lee, Kang Pa; Kim, Do-Yoon; Hwang, Dae Il; Won, Kyung-Jong; Lee, Dae Won; Lee, Hwan Myung

    2017-01-01

    Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an in-vivo wound healing assay in rat tail-skin. Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis. These results of our in-vitro and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine. Hisbiscus syriacus L. flower absolute increases HaCaT cell migration and proliferation. Hisbiscus syriacus L. flower absolute regulates phosphorylation of ERK 1/2 and Akt in HaCaT cell.Treatment with Hisbiscus syriacus L. flower induced sprout outgrowth.The wound in the tail-skin of rat was reduced by Hisbiscus syriacus L. flower absolute Abbreviations used: HSF: Hibiscus syriacus L. flower

  15. Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing

    Directory of Open Access Journals (Sweden)

    O. di Martino

    2017-01-01

    Full Text Available Higher plants are the source of a wide array of bioactive compounds that support skin integrity and health. Hibiscus syriacus, family Malvaceae, is a plant of Chinese origin known for its antipyretic, anthelmintic, and antifungal properties. The aim of this study was to assess the healing and hydration properties of H. syriacus ethanolic extract (HSEE. We established a cell culture from Hibiscus syriacus leaves and obtained an ethanol soluble extract from cultured cells. The properties of the extract were tested by gene expression and functional analyses on human fibroblast, keratinocytes, and skin explants. HSEE treatment increased the healing potential of fibroblasts and keratinocytes. Specifically, HSEE significantly stimulated fibronectin and collagen synthesis by 16 and 60%, respectively, while fibroblasts contractility was enhanced by 30%. These results were confirmed on skin explants, where HSEE accelerated the wound healing activity in terms of epithelium formation and fibronectin production. Moreover, HSEE increased the expression of genes involved in skin hydration and homeostasis. Specifically, aquaporin 3 and filaggrin genes were enhanced by 20 and 58%, respectively. Our data show that HSEE contains compounds capable of stimulating expression of biomarkers relevant to skin regeneration and hydration thereby counteracting molecular pathways leading to skin damage and aging.

  16. Cytotoxic effect of triterpenoids from the root bark of Hibiscus syriacus.

    Science.gov (United States)

    Shi, Li-Shian; Wu, Chao-Hsuan; Yang, Te-Chun; Yao, Chen-Wen; Lin, Hang-Ching; Chang, Wen-Liang

    2014-09-01

    In this study, 4 new triterpenoids-3β- acetoxy-olean-11-en,28,13β-olide (1), 3β- acetoxy-11α,12α-epoxy-olean-28,13β-olide (2), 19α-epi-betulin (3), and 20, 28-epoxy-17β,19β-lupan-3β-ol (4)-and 12 known compounds, were isolated from the root bark of Hibiscus syriacus L. by using acetone extraction. Their structures were characterized by extensive spectroscopic analysis. To investigate cytotoxicity, A549 human lung cancer cells were exposed to the extract and the compounds identified from it. Significantly reduced cell viability was observed with betulin-3-caffeate (12) (IC50, 4.3 μM). The results of this study indicate that betulin-3-caffeate (12) identified from H. syriacus L. may warrant further investigation for potential as anticancer therapies. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. [Chemical Constituents from Leaves of Hibiscus syriacus and Their α-Glucosidase Inhibitory Activities].

    Science.gov (United States)

    Wei, Qiang; Ji, Xiao-ying; Xu, Fei; Li, Qian-rong; Yin, Hao

    2015-05-01

    To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities. Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate. 15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15). All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.

  18. Paracoccus hibiscisoli sp. nov., isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus).

    Science.gov (United States)

    Lin, Pei; Yan, Zheng-Fei; Won, Kyung-Hwa; Yang, Jung-Eun; Li, Chang-Tian; Kook, MooChang; Wang, Qi-Jun; Yi, Tae-Hoo

    2017-07-01

    A Gram-reaction-negative, aerobic, non-motile, short-rod-shaped bacterium (THG-T2.31T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 10-35 °C (optimum 28 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-4.0 % NaCl (optimum 1.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.31T were identified as Paracoccus marcusii DSM 11574T (98.4 %), Paracoccus haeundaensis BC74171T (98.3 %), Paracoccus carotinifaciens E-396T (98.3 %), Paracoccus aestuarii B7T (97.3 %) and Paracoccus seriniphilus MBT-A4T (97.0 %); levels of similarity with the type strains of other species of the genus Paracoccus were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified phospholipids. The major fatty acids were C16 : 0, C18 : 0, C10 : 0 3-OH, and C18 : 1ω7c. The quinone was ubiquinone-10 (Q-10). The DNA G+C content of strain THG-T2.31T was 69.1 mol%. DNA-DNA hybridization values between strain THG-T2.31T and P. marcusii DSM 11574T, P. haeundaensis BC74171T, P. carotinifaciens E-396T, P. aestuarii B7T and P. seriniphilus MBT-A4T were 38.9 % (34.9 %, reciprocal analysis), 29.1 % (23.5 %), 28.0 % (19.7 %), 18.9 % (9.3) and 13.1 % (6.2 %). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T2.31T represents a novel species of the genus Paracoccus, for which the name Paracoccus hibiscisoli sp. nov. is proposed. The type strain is THG-T2.31T (=KACC 18933T=CCTCC AB 2016182T).

  19. Flavobacterium hibisci sp. nov., isolated from the rhizosphere of Hibiscus syriacus L.

    Science.gov (United States)

    Moya, Gabriela; Yan, Zheng-Fei; Trinh, Huan; Won, Kyung-Hwa; Yang, Jung-Eun; Kook, Moo-Chang; Yi, Tae-Hoo

    2017-04-01

    A Gram-stain-negative, smooth, bright-yellow-pigmented, rod-shaped bacterial strain, slightly motile by gliding, catalase- and oxidase-positive and aerobic, but growing weakly under anaerobic conditions, was isolated from the rhizosphere of the flower mugunghwa (Hibiscus syriacus L.) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. The strain named THG-HG1.4T grew at 15-35 °C, pH 6.5-9.0 and in the presence of 0-2.5 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequence showed that strain THG-HG1.4T was most closely related to Flavobacterium gyeonganense HME7524T (98.83 %) and Flavobacterium arsenitoxidans S2-3HT (97.28 %). The DNA G+C content of strain THG-HG1.4T was 41.2 mol%. In DNA-DNA hybridization, the DNA-DNA relatedness between strain THG-HG1.4T and its closest phylogenetic neighbour was below 64.1 %. The predominant isoprenoid quinone detected in strain THG-HG1.4T was menaquinone-6 (MK-6). The major polar lipids were found to be phosphatidylethanolamine, three unidentified lipids, two unidentified glycolipids and an unidentified aminolipid. The major fatty acids were identified as iso-C15 : 0, iso-C15 : 0 3-OH, C16 : 0, iso-C17 : 0 3-OH and summed feature 3. Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-HG1.4T, it has been concluded that the novel isolate represents a novel species of the genus Flavobacterium.Flavobacterium hibisci sp. nov. is proposed, with THG-HG1.4T (=KACC 18852T=CCTCC AB 2016178T) as the type strain.

  20. Deinococcus hibisci sp. nov., isolated from rhizosphere of Hibiscus syriacus L. (mugunghwa flower).

    Science.gov (United States)

    Moya, Gabriela; Yan, Zheng-Fei; Chu, Dong-Hun; Won, KyungHwa; Yang, Jung-Eun; Wang, Qi-Jun; Kook, Moo-Chang; Yi, Tae-Hoo

    2018-01-01

    A Gram-stain-positive, pink-pigmented, coccus-shaped, strictly aerobic, non-motile bacterium, strain THG-AG1.5 T , was isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, Republic of Korea. The isolated strain grew optimally at 25-30 °C, at pH 6.0-7.5 and in the presence of additional 0-1.5 % (w/v) NaCl. Strain THG-AG1.5 T exhibited tolerance to UV radiation (>1500 J m -2 ) and to gamma radiation (>12 kGy). Based on 16S rRNA gene sequence comparisons, strain THG-AG1.5 T was closely related to Deinococcus daejeonensis MJ27 T (98.03 %), Deinococcus radiotolerans C1 T (97.61 %) and Deinococcus grandis DSM 3963 T (97.32 %). The genomic DNA G+C content of strain THG-AG1.5 T was 74.8 mol%. The DNA-DNA hybridization values between strain THG-AG1.5 T and its closest phylogenetically neighbours were below 63.0 %. The peptidoglycan amino acids were alanine, valine, glutamic acid, glycine, ornithine, lysine and aspartic acid. Strain THG-AG1.5 T contained ribose, mannose and glucose as whole-cell-wall sugars and menaquinone-8 (MK-8) as the only isoprenoid quinone. The major component in the polyamine pattern was spermidine. The major polar lipids of strain THG-AG1.5 T were a phosphoglycolipid, six unidentified glycolipids and an unidentified aminophospholipid. The major fatty acids were identified as iso-C15 : 0, C15 : 1ω6c, C16 : 0, iso-C17 : 0, C17 : 0, C18 : 0 and summed feature 3. On the basis of our polyphasic taxonomy study, strain THG-AG1.5 T represents a novel species within the genus Deinococcus, for which the name Deinococcushibisci sp. nov. is proposed. The type strain is THG-AG1.5 T (=KACC 18850 T =CCTCC AB 2016078 T ).

  1. Roseomonas hibiscisoli sp. nov., isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus).

    Science.gov (United States)

    Yan, Zheng-Fei; Lin, Pei; Li, Chang-Tian; Kook, MooChang; Wang, Qi-Jun; Yi, Tae-Hoo

    2017-08-01

    A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 20-40 °C (optimum 28 °C), at pH 5-9 (optimum 7) and with 0-4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22T were identified as Roseomonas rhizosphaerae YW11T (98.5 % similarity), Roseomonas rubra S5T (98.5 %), Roseomonas cervicalis ATCC 49957T (98.2 %), Roseomonas aestuarii JC17T (97.8 %), Roseomonas oryzae JC288T (97.3 %) and Roseomonas ludipueritiae 170/96T (97.3 %); levels of similarity with the type strains of other Roseomonas species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, three unidentified phospholipids and three unidentified lipids. The major quinone was ubiquinone-10. The major fatty acids were C16 : 0, C18 : 1 2-OH, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The DNA G+C content of strain THG-N2.22T was 64.1 mol%. DNA-DNA hybridization values between strain THG-N2.22T and R. rhizosphaerae YW11T, R. rubra S5T, R. cervicalis ATCC 49957T, R. aestuarii JC17T, R. oryzae JC288T and R. ludipueritiae 170/96T were 43.1 % (30.2 %, reciprocal analysis), 39.0 % (24.7 %), 34.4 % (15.2 %), 18.0 % (14.5 %), 14.7 % (9.7 %) and 11.0 % (5.6 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-N2.22T represents a novel species of the genus Roseomonas, for which the name Roseomonas hibiscisoli sp. nov. is proposed. The type strain is THG-N2.22T (=KACC 18935T=CCTCC AB 2016176T).

  2. Caulobacter hibisci sp. nov., isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower).

    Science.gov (United States)

    Moya, Gabriela; Yan, Zheng-Fei; Won, KyungHwa; Yang, Jung-Eun; Wang, Qi-Jun; Kook, MooChang; Yi, Tae-Hoo

    2017-09-01

    A Gram-stain-negative, smooth, bright yellow-pigmented, aerobic, catalase- and oxidase-positive and rod-shaped bacterial strain was isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. Cells were dimorphic, non-motile or non-stalked, and motile by means of peritrichous flagellum. The strain, named THG-AG3.4T, grew at 15-35 °C, at pH 6.5-9.0 and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-AG3.4T was most closely related to Caulobacter segnis ATCC 21756T (98.64 % similarity), Caulobacter vibrioides CB51T (98.57 %) and Caulobacter henricii ATCC 15253T (97.41 %). The DNA G+C content of strain THG-AG3.4T was 64.0 mol%. In DNA-DNA hybridization, the DNA-DNA relatedness between strain THG-AG3.4T and its closest phylogenetic neighbour was below 55.0 %. The predominant isoprenoid quinone detected in strain THG-AG3.4T was ubiquinone-10 (Q-10). The major polar lipids were found to be an unidentified lipid, two unidentified phosphoglycolipids, five unidentified glycolipids, eight unidentified aminolipids and phosphatidylglycerol. The major fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). Thus, based on the report of the phenotypic, genotypic and phylogenetic characterization of strain THG-AG3.4T, it has been concluded that the isolate represents a novel species of the genus Caulobacter, for which the name Caulobacter hibisci sp. nov. is proposed. The type strain is THG-AG3.4T (=KACC 18849T=CCTCC AB 2016077T).

  3. Effects of 60Co γ-rays irradiation on germination and seedling growth of Hibiscus syriacus

    International Nuclear Information System (INIS)

    Li Xiufen; Wu Fulan; Zhang Deshun; Meng Zhennong; Cao Jiyun

    2009-01-01

    The seeds of Hibiscus syria cus were irradiated by 60 Co γ-rays at dose rate of 50 ∼ 500Gy/h for 0.5 and 1h, the seed germination rate and the seedling growth characters were surveyed. The results indicated that the seed germination was promoted when the absorbed dose was below 200Gy, and the seedling survival rate was increased when the dose was below 100Gy. The germination was inhibited, leaves and secondary roots were difficult to form, and the seedlings blasting occurred when the dose was over 200Gy. The dose rate affects the irradiation results. Absorbed dosed of 50 ∼ 100Gy were suggested for increasing seedling growth rate and 100 ∼ 200Gy were recommend for mutagenesis. (authors)

  4. Sortiment Hibiscus in beweging

    NARCIS (Netherlands)

    Hoffman, M.H.A.

    2010-01-01

    Hibiscus is hot. Op Plantarium 2009 werden maar liefst acht nieuwe cultivars ter keuring aangeboden. Bovendien winnen de laatste jaren ook andere soorten dan de bekende Hibiscus syriacus aan populariteit

  5. Hibiscus

    Science.gov (United States)

    ... uterus; reduce swelling; and work like antibiotics to kill bacteria and worms. ... Herbs and supplements that might lower blood pressureHibiscus may lower blood pressure. Using it along with other ...

  6. Free radical scavenging activities of pigment extract from Hibiscus ...

    African Journals Online (AJOL)

    The macroporous resin adsorption method was used to purify the pigment from Hibiscus syriacus L. petals. Through the comparison of the adsorption and desorption rates of six types of macroporous resins including AB-8, S-8, NKA-9, DM-130, D101 and 860021 to the pigment, 860021 resin was selected as the most ...

  7. Hibiscus sabdari

    African Journals Online (AJOL)

    AISA

    3Laboratoire de Biotechnologie et Physiologie Végétales de l'Université de Bretagne Occidentale, Isamor/. Esmisab Technopole Brest Iroise 29280 Plouzané (France). RESUME. L'oseille de Guinée (Hibiscus sabdariffa L.) est une malvaceae cultivée essentiellement pour l'alimentation et pour la fabrication de fibres ...

  8. Hibiscus sabdariffa L

    African Journals Online (AJOL)

    TEKWA IJASINI JOHN

    Hibiscus sabdariffa L.) and its mode of inheritance with special reference to the variation intermedius. Genetica,. 43: 366-374. Aliyu, L., 1998. Roselle (Hibiscus sabdariffa L.). Production as affected by pruning and sowing date. Journal of Agric.

  9. Hibiscus tiliaceus L

    Science.gov (United States)

    James A. Allen

    2002-01-01

    The genus Hibiscus includes about 200 species distributed primarily in the tropical and subtropical regions of the world. It is a highly variable genus, with relatively few characteristics in common (Wagner and others 1990). Hibiscus tiliaceus is one of the most widespread species in the genus, with a pantropical, mainly...

  10. Hibiscus acetosella 'Sahara Sunset'

    Science.gov (United States)

    The Agricultural Research Service, United States Department of Agriculture, hereby release to nurserymen a new African Hibiscus, Hibiscus acetosella Welw. ex Hiern., named ‘Sahara Sunset’. This cultivar, tested as HAC06-11 was selected from a group seedlings grown at the Thad Cochran Southern Horti...

  11. Developmental patterns in two populations of the millipede Archispirostreptus syriacus (De Saussure) in Israel (Diplopoda)

    NARCIS (Netherlands)

    Bercovitz, Klara; Warburg, M.R.

    1985-01-01

    Comparison between two populations of the spirostreptid millipede Archispirostreptus syriacus reveals that there are no significant differences in the pattern of development between the xeric population (in Brosh) and the mesic one (in Megiddo). However, there is a difference in the duration of

  12. Verbeter Hibiscus met voeding

    NARCIS (Netherlands)

    Aendekerk, T.G.L.

    2003-01-01

    Onderzoek naar de invloed van pH en de voedingsoplossing op groeiafwijkingen bij Hibiscus. Gegevens in bijgaande tabellen: 1) Gebruikte voedingsoplossingen (waarden in mmol/l); 2) Beoordeling bij gebruikte voedingsoplossingen; 3) Optimale voedingsoplossingen (waarden in mmol/l) in twee periodes; 4)

  13. Crystal structures of hibiscus acid and hibiscus acid dimethyl ester isolated from Hibiscus sabdariffa (Malvaceae)

    OpenAIRE

    Zheoat, Ahmed M.; Gray, Alexander I.; Igoli, John O.; Kennedy, Alan R.; Ferro, Valerie A.

    2017-01-01

    The biologically active title compounds have been isolated from Hibiscus sabdariffa plants, hibiscus acid as a dimethyl sulfoxide monosolvate [systematic name: (2S,3R)-3-hy?droxy-5-oxo-2,3,4,5-tetra?hydro?furan-2,3-di?carb?oxy?lic acid dimethyl sulfoxide monosolvate], C6H6O7?C2H6OS, (I), and hibiscus acid dimethyl ester [systematic name: dimethyl (2S,3R)-3-hy?droxy-5-oxo-2,3,4,5-tetra?hydro?furan-2,3-di?carboxyl?ate], C8H10O7, (II). Compound (I) forms a layered structure with alternating laye...

  14. Sortiment Hibiscus voortdurend in beweging

    NARCIS (Netherlands)

    Hoffman, M.H.A.

    2010-01-01

    Hibiscus is hot en volop in ontwikkeling. Op Plantarium 2009 werden maarliefst acht nieuwe cultivars ter keuring aangeboden. In naamlijsten van 2005 staan ruim 150 cultivars geregistreerd, terwijl dit er in de naamlijst van 1995 slechts 60 waren. Alle reden om op een rijtje te zetten wat er bekend

  15. Ozone bioindicator sampling and estimation

    Science.gov (United States)

    Gretchen C, Smith; William D. Smith; John W. Coulston

    2007-01-01

    Ozone is an important forest stressor that has been measured at known phytotoxic levels at forest locations across the United States. The percent forest exhibiting negative impacts from ozone air pollution is one of the Montreal Process indicators of forest health and vitality. The ozone bioindicator data of the U.S. Forest Service Forest Inventory and Analysis Program...

  16. Phytochemical Screening and Antibacterial Activities Of HIBISCUS ...

    African Journals Online (AJOL)

    USER

    46. Nigerian Journal of Chemical Research. Vol. 20, 2015. Phytochemical Screening and Antibacterial Activities Of HIBISCUS SABDARIFFA L. Leaf Extracts. Adamu, H. ... extract of the leaves of Hibiscus sabdariffa L. The mean zones of inhibition of the methanolic leaf extracts ... drink is popularly known as zobo in Nigeria.

  17. Direct genetic transformation of Hibiscus sabdariffa L.

    African Journals Online (AJOL)

    Administrator

    After 60 days evaluation point, the assessment of the transformation by PCR revealed that H. sabdariffa line tested, carried the nptII gene. Key words: Hibiscus sabdariffa, genetic transformation. INTRODUCTION. Hibiscus sabdariffa is a crop widely cultivated in Sub. Saharan Africa, growing on sandy soils after the harvest.

  18. Microbial composition of guava (Psidium guajava), hibiscus ...

    African Journals Online (AJOL)

    Microbial composition of guava (Psidium guajava), hibiscus (Hibiscus-rosa sinensis), mango (Mangifera indica) and pumpkin (Telfairia occidentalis Hook) ... African Journal of Biotechnology ... The microbial genera isolated from this study showed that, both human and plant pathogens can colonize plants' phyllosphere.

  19. Crystal structures of hibiscus acid and hibiscus acid dimethyl ester isolated from Hibiscus sabdariffa (Malvaceae).

    Science.gov (United States)

    Zheoat, Ahmed M; Gray, Alexander I; Igoli, John O; Kennedy, Alan R; Ferro, Valerie A

    2017-09-01

    The biologically active title compounds have been isolated from Hibiscus sabdariffa plants, hibiscus acid as a dimethyl sulfoxide monosolvate [systematic name: (2 S ,3 R )-3-hy-droxy-5-oxo-2,3,4,5-tetra-hydro-furan-2,3-di-carb-oxy-lic acid dimethyl sulfoxide monosolvate], C 6 H 6 O 7 ·C 2 H 6 OS, (I), and hibiscus acid dimethyl ester [systematic name: dimethyl (2 S ,3 R )-3-hy-droxy-5-oxo-2,3,4,5-tetra-hydro-furan-2,3-di-carboxyl-ate], C 8 H 10 O 7 , (II). Compound (I) forms a layered structure with alternating layers of lactone and solvent mol-ecules, that include a two-dimensional hydrogen-bonding construct. Compound (II) has two crystallographically independent and conformationally similar mol-ecules per asymmetric unit and forms a one-dimensional hydrogen-bonding construct. The known absolute configuration for both compounds has been confirmed.

  20. 'Lufkin Red' and 'Lufkin White' winter-hardy hibiscus (Hibiscus x laevis All.)

    Science.gov (United States)

    USDA ARS announces the release of ‘Lufkin Red’ and ‘Lufkin White’ winter-hardy native hibiscuses.Both clones have desirable horticultural traits in combination with demonstrated high levels of field resistance to the leaf spot complex that is problematic on winter-hardy hibiscus clones in areas wit...

  1. Starch-Glycerol Based Edible Film and Effect of Rosella (Hibiscus Sabdariffa Linn Extract and Surimi Dumbo Catfish (Clarias gariepinus Addition on Its Mechanical Properties

    Directory of Open Access Journals (Sweden)

    Endaruji Sedyadi

    2016-10-01

    Full Text Available Effect of Rosella (Hibiscus Sabdariffa Linn Extract and Surimi Dumbo catfish (Clarias gariepinus addition on Starch-Based Edible Film-Glycerol Mechanical Properties has been done. The purpose of this study is to create an active environment-friendly packaging material. Surimi additions are intended to improve the mechanical properties of bioplastics and additions of Rosella extract intended as a bio-indicator of acidity. The method used was Solvent Casting. An amount of surimi and rosella extract varied to obtain the best mechanical properties. The results shows that the addition of surimi and rosella flower extract significantly effect the elongation of Edible films produced up to 27%.

  2. Bioindication Potential of the Coleoptera

    Directory of Open Access Journals (Sweden)

    Belitskaya Mariya Nikolaevna

    2015-09-01

    Full Text Available Different families of Coleoptera ambiguously respond to the pollution of SPZs with industrial emissions. For example, the SPZ of Volgograd aluminum plant has the changing biodiversity of insect communities at different distances from the pollution source. The increasing level of pollution is accompanied by the reduction in species abundance. At a distance of 200 m a special ecological zone with the specific composition of the entomofauna was formed. It is significantly different from other habitats. No Cerambycidae species may survive in the zone of maximum pollution, and the number of Curculionidae species is reduced significantly. The number of Cerambycidae decreases by more than 40 % in the presence of even minimal contamination. The most sensitive bioindicators are represented by such insects as Cerambycidae, Curculionidae and Chrysomelidae. Changes in the indices can be described by the function y = arctan (x, where x is the distance from the pollution source (in meters. The specificity of this function is to identify levels of possible changes of species richness and numerical abundance of communities. On the basis of trigonometric functions describing the changes in the species composition and abundance, the authors offered the method for assessing the quality of the environment in SPZs. The use of three families of insects opens up prospects of differentiation zones of technogenic pressure.

  3. Studying the antibacterial effects of some phytochemical compounds isolated from Origanum syriacum, Thymus syriacus and Myrtus communis on some bacteria

    International Nuclear Information System (INIS)

    Almariri, I.; Swied, G.; Oda, A.

    2012-01-01

    Summary The Origanum Syriacum, Thymus syriacus and Myrtus communis leaves samples were collected. Essential oils (EO) extractions were done by hydrodistillation in a Clevenger apparatus. GC-FID was used to analyze the essential oils and GC-MS was used to confirm the identities of the isolated components. Preparative HPLC technique was used to separate and isolate the oils main components. The bulk EO and the separated components were tested for their antibacterial activities against Escherichia coli O157, Salmonella typhimurium, Klebsiella pneumonia, Yersinia enterocolitica O9, Brucella melitensis, Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus using broth microdilution method. The results confirm that the EOs and some of its components exhibited significant bactericidal activity. (author)

  4. The Hibiscus panduriformis complex (Malvaceae) in Australia

    NARCIS (Netherlands)

    Juswara, L.S.; Craven, L.A.

    2005-01-01

    The Hibiscus panduriformis Burm.f. species complex in Australia is revised. Six species are recognised, of which one is the widespread H. panduriformis; one, H. austrinus, is based upon H. panduriformis var. australis; and four represent new species, H. apodus, H. calcicola, H. fluvialis, and H.

  5. Phytochemical Screening and Antibacterial Activities of Hibiscus ...

    African Journals Online (AJOL)

    The phytochemical properties and the antibacterial potency of rosselle (Hibiscus sabdariffa L) leaf extracts were evaluated using the cold maceration method, agar diffusion method and qualitative phytochemical analysis respectively. The methanolic extract was tested against Salmonella typhi, Escherichia coli and ...

  6. Detection of somaclonal variation in micropropagated Hibiscus ...

    African Journals Online (AJOL)

    The main objective of micropropagation is to produce clones i.e. plants which are phenotypically and genetically identical to the mother plants. The culture of organized meristems usually guarantees the production of true-to-type plants but variations in the progenies have been widely reported. Hibiscus sabdariffa L. plants ...

  7. Can hibiscus tea lower blood pressure

    Science.gov (United States)

    Hibiscus sabdariffa is a common ingredient found in blended herbal teas, and beverages made from the dried calyces of this plant are popular worldwide. In vitro studies have shown that H. sabdariffa has antioxidant properties and, in animal models of hypertension, extracts of this plant lower blood ...

  8. Preliminary characterization of residual biomass from Hibiscus ...

    African Journals Online (AJOL)

    Hibiscus sabdariffa calyces are mainly used for different agro-food and beverages applications. The residual biomass generated contains various useful substances that were extracted and characterized. It contained 23% (w/w) soluble pectic material, a food additive, extracted with hot acidified water (80°C, pH = 1.5) and ...

  9. Comparative evaluation of bioactive compounds in Hibiscus ...

    African Journals Online (AJOL)

    There is growing interest in the chemical composition of plants towards discovery of more effective biotherapeutic agents. Six bioactive compounds were evaluated from Hibiscus sabdariffa and Syzygium samarangense juice extracts. Both juices had high amounts of saponins, with Syzygium samarangense having higher ...

  10. Inheritance of characters in kenaf (Hibiscus cannabinus)

    African Journals Online (AJOL)

    SERVER

    2008-04-03

    David and Adam, 1988). The plant belongs to the order Malvales, the family Malvaceae and the genus Hibiscus ... The varieties in relation to the character studied are as follows: NRG-NG-R5: Pink flowers with resistance to root ...

  11. Bioindicators used in aquatic and terrestrial monitoring

    Science.gov (United States)

    Melancon, Mark J.; Hoffman, David J.; Rattner, Barnett A.; Burton, G. Allen; Cairns, John

    1995-01-01

    There are many different types of contaminants present in the environment ranging from synthetic chemicals, which would not be present in the environment without man's intervention, to trace metals that are required for life. Concerns range from possible harmful effects on flora and fauna to possible harm to humans consuming such organisms. The complete chemical analysis for all possible environmental contaminants in sediments, water, air, and every species and sample of interest would not only be excessively costly, but the facilities to handle do many samples do not exist. Such chemical analysis would only establish the presence of contaminants, without revealing how available or active they were within the organism.Bioindicators, sometimes called biomarkers, are responses in living organisms that may simply signify exposure to contaminants, may predict future harm, or may themselves be harmful effects. In this chapter, a bioindicator will be interpreted to mean the same as a biomarker (a biochemical, physiological, or morphological response), but not a population or ecosystem bioindicator such as a sentinel species, genetic variability, or species richness. thus, a bioindicator (biomarker) may be studied in a particular species to assess the status of that species, or in a sentinel species to assess habitat status. Individuals of the sentinel species might be those already residing at the site of interest or might be pristine organisms brought to the site and maintained in cages (fish) or released and recovered (birds).

  12. Hibiscus plant named `Sahara Sunset` U.S. Plant Patent 21,765

    Science.gov (United States)

    'Sahara Sunset' is a new and distinct cultivar of Hibiscus, botanically known as Hibiscus acetosella. The new Hibiscus was originated in Poplarville, Miss. and is a product of a mutation induction program. The parent of the present new cultivar is an unknown Hibiscus acetosella Wels. Ex Hiern seedli...

  13. PHARMACOLOGICAL EFFECTS AND THERAPEUTIC PROPERTIES OF HIBISCUS CANNABINUS- A REVIEW

    OpenAIRE

    Ali Esmail Al-Snafi

    2018-01-01

    The phytochemical analysis of Hibiscus cannabinus showed the presence of phytosterols, flavonoids, polyphenols, tannins, steroids, alkaloids, saponins, lignans, essential oils, glucosides such as cannabiscitrin, cannabiscetin and anthocyanin glycoside. The pharmacological studies revealed that Hibiscus cannabinus possessed cytotoxic, anthelmintic, antibacterial, antiulcer, antidiabetic, hypolipidemic, antioxidant, immunological, haematinic and hepatoprotective effects. This review will highli...

  14. Effects of aqueous extracts of Hibiscus sabdariffa calyces and ...

    African Journals Online (AJOL)

    The effects of aqueous extracts of the leaves of Ocimum gratissimum and calyces of Hibiscus sabdariffa on intestinal transit were determined in experimental rats The leaves of Ocimum gratissimum were oven dried and then pulverized. The dried calyces of Hibiscus sabdariffa were also pulverized. 10% extracts of both ...

  15. Effect of Hibiscus sabdariffa calyx extract on stressed rabbit plasma ...

    African Journals Online (AJOL)

    Hibiscus sabdariffa aqueous extract has been shown to have antioxidant and lipid lowering effects in animal studies. This study is aimed at investigating the effects of the aqueous extract of Hibiscus sabdariffa calyx on the lipid profile of rabbits subjected to stress. The rabbits were stressed by suspension for 2 minutes, twice ...

  16. Effects of Hibiscus Sabdariffa on the Heamostatic and ...

    African Journals Online (AJOL)

    The potential haematological effects associated with the administration of water leaf extract of Hibiscus sadariffa was investigated in rats. Results showed that Hibiscus sabdariffa decrease haemoglobin concentration, packed cell volume, white blood cell count and platelet count without having any haemostatic effect on ...

  17. A cilevirus infects ornamental hibiscus in Hawaii.

    Science.gov (United States)

    Melzer, Michael J; Simbajon, Nelson; Carillo, James; Borth, Wayne B; Freitas-Astúa, Juliana; Kitajima, Elliot W; Neupane, Kabi R; Hu, John S

    2013-11-01

    The complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3' termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92 % identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genus Cilevirus.

  18. Mutagenesis of hibiscus rosa-sinensis

    International Nuclear Information System (INIS)

    Shuhaimi Shamsudin; Shakinah Salleh; Mohd Nazir Basiran

    2005-01-01

    Mutation induction is an alternative method to create more variation in Hibiscus rosa-sinensis. Radiosensitivity test was carried out to determine the effective doses for irradiation of stem cuttings. Stem cuttings of Hibiscus rosa-sinensis were irradiated at 10, 20, 30 and 60 Gy using a gamma cell with a Co-60 source at a dose rate of 1.66 Gys -1 . Irradiated stem cuttings were planted in sand-beds and data on the number of growing shoots were taken. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. It was shown that the LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three variants with variation in flower shapes and colours were obtained. (Author)

  19. 'USS Alabama', 'USS Mississippi', 'USS Missouri', 'USS Tennessee' and 'USS Texas' Chinese hibiscus (Hibiscus rosa-sinenis L.)

    Science.gov (United States)

    Five new Chinese hibiscus cultivars are products from a planned hibiscus breeding program conducted by the inventors at Poplarville, MS. The goal of the research is to produce new clones with a combination of desirable horticultural traits. Desirable floral traits include early bloom, uniform flower...

  20. Raman spectroscopy for the control of the atmospheric bioindicators

    Science.gov (United States)

    Timchenko, E. V.; Timchenko, P. E.; Shamina, L. A.; Zherdeva, L. A.

    2015-09-01

    Experimental studies of optical parameters of different atmospheric bioindicators (arboreous and terricolous types of plants) have been performed with Raman spectroscopy. The change in the optical parameters has been explored for the objects under direct light exposure, as well as for the objects placed in the shade. The age peculiarities of the bioindicators have also been taken into consideration. It was established that the statistical variability of optical parameters for arboreous bioindicators was from 9% to 15% and for plants from 4% to 8.7%. On the basis of these results dandelion (Taraxacum) was chosen as a bioindicator of atmospheric emissions.

  1. Bioindication in Urban Soils in Switzerland

    Science.gov (United States)

    Amossé, J.; Le Bayon, C.; Mitchell, E. A. D.; Gobat, J. M.

    2012-04-01

    Urban development leads to profound changes in ecosystem structure (e.g. biodiversity) and functioning (e.g. ecosystem services). While above-ground diversity is reasonably well studied much less is known about soil diversity, soil processes and more generally soil health in urban settings. Soil invertebrates are key actors of soil processes at different spatial and temporal scales and provide essential ecosystem services. These functions may be even more vital in stressed environments such as urban ecosystems. Despite the general recognition of the importance of soil organisms in ecosystems, soil trophic food webs are still poorly known and this is especially the case in urban settings. As urban soils are characterised by high fragmentation and stress (e.g. drought, pollution) the structure and functioning of soil communities is likely to be markedly different from that of natural soils. It is for example unclear if earthworms, whose roles in organic matter transformation and soil structuration is well documented in natural and semi-natural soils, are also widespread and active in urban soils. Bioindication is a powerful tool to assess the quality of the environment. It is complementary to classical physicochemical soil analysis or can be used as sole diagnostic tool in cases where these analyses cannot be performed. However little is known about the potential use of bioindicators in urban settings and especially it is unclear if methods developped in agriculture can be applied to urban soils. The development of reliable methods for assessing the quality of urban soils has been identified as a priority for policy making and urban management in Switzerland, a high-urbanized country. We therefore initiated a research project (Bioindication in Urban Soil - BUS). The project is organised around four parts: (i) typology of urban soils in a study Region (Neuchâtel), (ii) sampling of soil fauna and analysis of soil physicochemical properties, (iii) comparison of the

  2. Antioxidant and drug detoxification potentials of Hibiscus sabdariffa anthocyanin extract.

    Science.gov (United States)

    Ajiboye, Taofeek O; Salawu, Nasir A; Yakubu, Musa T; Oladiji, Adenike T; Akanji, Musbau A; Okogun, Joseph I

    2011-04-01

    The antioxidant and drug metabolizing potentials of Hibiscus anthocyanin extract in CCl(4)- induced oxidative damage of rat liver was investigated. Hibiscus anthocyanin extract effectively scavenge α-diphenyl-β-picrylhydrazyl (DPPH) radical, superoxide ion, and hydrogen peroxide. It produced a 92% scavenging effect of DPPH radical at a concentration of 2.0 mg/mL. Hibiscus anthocyanin extract produced a 69 and 90% scavenging effect on superoxide ion and hydrogen peroxide, respectively, at 1.0 mg/mL, which compared favorably with the synthetic antioxidant (butylated hydroanisole and α-tocopherol). A reducing power of this anthocyanin was examined using K(3)Fe(CN)(6). Hibiscus anthocyanin extract has reducing power that is approximately 2-fold that of the synthetic antioxidant, butylated hydroanisole. Hibiscus anthocyanin extract produced a significantly increase and completely attenuated the CCl(4)-mediated decrease in antioxidant enzymes (e.g., catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase). However, the level of nonenzymic antioxidant molecules (i.e., vitamins C and E) were significant preserved by Hibiscus anthocyanin extract. There was an induction of phase II drug-detoxifying enzymes: glutathione S-transferase, NAD(H):quinone oxidoreductase, and uridyl diphosphoglucuronosyl transferase by 65, 45, and 57%, respectively. In view of these properties, Hibiscus sabdariffa anthocyanin extract can act as a prophylactic by intervening as a free radical scavenger both in vitro and in vivo as well as inducing the phase II drug detoxification enzymes.

  3. Ecosystems for Early Warning: Potential Use of Bioindicators

    Science.gov (United States)

    Zommers, Z. A.; Sitati, A. M.; Habilov, M.

    2014-12-01

    Bioindicators are biological processes, species or communities, which are used to assess changes in the environment or environmental quality. Theoretically, they could also be used to provide advanced warning of hazards. They are inexpensive, locally relevant, and can encourage stakeholder participation in early warning system development and maintenance. While bioindicators have been identified for environmental problems such as air pollution and water pollution, and have been used to assess health of ecosystems, little information is available on bioindicators for climate related hazards. This presentation reviews possible biodindicators for droughts, wildfires and tropical cyclones, based on the results of a literature review. It will also present results from a household survey of 36 communities in Kenya, Ghana and Burkina Faso. Indigenous knowledge offers a wealth of potential bioindicators; including animal and insect behavior, and plant phenology. Yet significant study is needed to verify these indicators and evaluate them against criteria such as specificity, variability, monotonicity, practicality and relevance. Bioindicators may not be specific to individual hazards and may provide limited advanced warning, as response often occurs after the actual onset of the hazard. Furthermore, indicators may become increasingly unreliable due to climate change itself. There is a need for a large-scale assessment of hazard bioindicators, which should also include forecasts of bioindicator change under global warming, and a cost-benefit analysis of the value of integrating bioindicators into early warning systems. Lessons can be drawn from ethnopharmacology. Coordinated research on this topic could contribute to the resilience of both ecosystems and human livelihoods.

  4. Aqueous extract of Hibiscus sabdarrifa calyx alleviates anemia and ...

    African Journals Online (AJOL)

    Aqueous extract of Hibiscus sabdarrifa calyx alleviates anemia and organ damage in Trypanosoma brucei brucei infected rats. IA Umar, E Daikwo, NG Maryoms, A Gidado, LB Buratai, FS Saka, MA Ibrahim ...

  5. Identifying salt stress-responsive transcripts from Roselle ( Hibiscus ...

    African Journals Online (AJOL)

    Hibiscus sabdariffa L.). Identifying the potentially novel transcripts responsible for salt stress tolerance in roselle will increase knowledge of the molecular mechanism underlying salt stress responses. In this study, differential display reverse ...

  6. Alpine Soils as long-term Bioindicators

    Science.gov (United States)

    Nestroy, O.

    2009-04-01

    Alpine soils as long-term bioindicators The introductory words concern the definitions and peculiarities of alpine soils and their position in the Austrian Soil Classification 2000 in comparison with the World Reference Base for Soil Resources 2006. The important parameters for genesis and threats for these soils in steep and high positions are discussed. It must be emphasized that the main threats are the very different kinds of erosion e.g. by water, wind and snow, and also by skiing (end of season) as well as and mountain-biking (mainly summer-sport). Due the very slow regeneration and - in this connection - due to the very slow changes of the soil entities, these soils give an utmost importance as a long-time bioindicator. With regard to the climate change one can assume an increase in the content of organic matter on site, but also an increase of erosion and mass movement on the other site, e. g. in kind of "plaiken" (soil slide) as result of an increasing intensity of rainfall. It lies partly in our hands to diminish the number and the intensity of the threats, we can influence the soil development, but the result to reach a new ecological equilibrium is very long - in case of alpine soil more than two generations.

  7. Study of large nonlinear change phase in Hibiscus Sabdariffa

    Science.gov (United States)

    Trejo-Durán, M.; Alvarado-Méndez, E.; Andrade-Lucio, J. A.; Rojas-Laguna, R.; Vázquez-Guevara, M. A.

    2015-09-01

    High intensities electromagnetic energy interacting with organic media gives rise to nonlinear optical effects. Hibiscus Sabdariffa is a flower whose concentrated solution presents interesting nonlinear optical properties. This organic material shows an important self-phase modulation with changes bigger than 2π. We present a diffraction ring patterns study of the Hibiscus Sabdariffa solution. Numerical results of transmittance, with refraction and simultaneous absorption, are shown.

  8. Effect of Hibiscus sabdariffa on obesity in MSG mice.

    Science.gov (United States)

    Alarcon-Aguilar, Francisco J; Zamilpa, Alejandro; Perez-Garcia, Ma Dolores; Almanza-Perez, Julio C; Romero-Nuñez, Eunice; Campos-Sepulveda, Efrain A; Vazquez-Carrillo, Laura I; Roman-Ramos, Ruben

    2007-10-08

    The aim of the present investigation was determine whether a standardized Hibiscus sabdariffa calyces aqueous extract has an effect on body weight in an obese animal model induced by the administration of monosodium glutamate. Hibiscus sabdariffa aqueous extract, containing 33.64 mg of total anthocyanins per each 120 mg of extract, was orally administered (120 mg/kg/day) for 60 days to healthy and obese mice, and body weight gain, food and liquid intake, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol, and triglycerides levels were measured. Hibiscus sabdariffa administration significantly reduced body weight gain in obese mice and increased liquid intake in healthy and obese mice. ALT levels were significantly increased on the 15th and 45th days in obese mice, but AST levels did not show significant changes. Mortality was not observed in the Hibiscus sabdariffa treated groups. Triglycerides and cholesterol levels showed non-significant reductions in animals treated with Hibiscus sabdariffa. Our data confirm the anti-obesity effect of Hibiscus sabdariffa reported by the Mexican population.

  9. Biological and molecular detection of Hibiscus chlorotic ringspot virus infecting Hibiscus rosa-sinensis in Iran

    Directory of Open Access Journals (Sweden)

    Reza POURRAHIM

    2014-01-01

    Full Text Available During 2011, ten leaf samples of Hibiscus rosa-sinensis with virus-like symptoms including diffuse mottling, numerous chlorotic spots and chlorotic ring spots, were collected from Guilan province, North Iran. The results of mechanical inoculation of sap from these leaves onto different indicator hosts showed local lesions (chlorotic or necrotic on Gompherena globosa, Chenopodium amaranticolor, C. quinoa and Gossypium hirsutum, while Hibiscus cannabinus (kenaf showed systemic chlorotic ring spot symptoms. Purified preparations of the isolated virus contained isometric particles approximately 28–30 nm in diameter. A specific band of about 1.3 kb was amplified from all symptomatic leaves using Hibiscus chlorotic ringspot virus (HCRSV specific primers. Comparative sequence analysis revealed that the IRN.HCRSV (JX865593 isolate shared the greatest nucleotide sequence identity (96.5% with NC-003608 (Singapore and the least nt sequence identity (91.5% with DQ392986 (Taiwan isolates. The phylogenetic tree showed at least two subgroups for HCRSV isolates in which the Iranian isolate was grouped with Singapore HCSRV (NC-003608. This is the first report of HCRSV in H. rosa-sinensis in Iran.

  10. The uses of bioindicators in radionuclide contamination assessment

    International Nuclear Information System (INIS)

    McGee, E. J.; McGarry, A.

    1994-01-01

    This paper is a brief review and discussion of some approaches to sampling using bioindicators that have been employed in the assessment of radionuclide contamination in Ireland. Studies by other researchers are also referred to and the fields of research in radioecology where the use of bioindicators could be further developed are discussed. The review includes a discussion of the important attributes of bioindicators and how they can best be used in the development of effective but economical sampling strategies that yield the maximum amount of information. (author)

  11. Important bioindicators for health management in Romania

    Directory of Open Access Journals (Sweden)

    Barliba I.

    2014-01-01

    Full Text Available Performance measurement is a coherent, robust, integrated, purposeful, comprehensive, efficient and transparent system. The evaluation of healthcare performance in Romania is based on four categories of bioindicators: human resources, use of services, economic and financial aspects, as well as quality. In this work, we were mainly interested in analyzing and describing these parameters. In order to illustrate the applicability of the hospital performance indicators, we considered the results obtained for these indices from the managers of three hospitals of the same level from Romania, the “Filişanilor” Hospital from Filiaşi, the Rovinari City Hospital and the Şegarcea City Hospital, and herein present them.

  12. Bio-indicators for radiation dose assessment

    International Nuclear Information System (INIS)

    Trivedi, A.

    1990-12-01

    In nuclear facilities, such as Chalk River Laboratories, dose to the atomic radiation workers (ARWs) is assessed routinely by using physical dosimeters and bioassay procedures in accordance with regulatory recommendations. However, these procedures may be insufficient in some circumstances, e.g., in cases where the reading of the physical dosimeters is questioned, in cases of radiation accidents where the person(s) in question was not wearing a dosimeter, or in the event of a radiation emergency when an exposure above the dose limits is possible. The desirability of being able to assess radiation dose on the basis of radio-biological effects has prompted the Dosimetric Research Branch to investigate the suitability of biological devices and techniques that could be used for this purpose. Current biological dosimetry concepts suggest that there does not appear to be any bio-indicator that could reliably measure the very low doses that are routinely measured by the physical devices presently in use. Nonetheless, bio-indicators may be useful in providing valuable supplementary information in cases of unusual radiation exposures, such as when the estimated body doses are doubtful because of lack of proper physical measurements, or in cases where available results need to be confirmed for medical treatment plannings. This report evaluates the present state of biological dosimetry and, in particular, assesses the efficiency and limits of individual indicators. This has led to the recommendation of a few promising research areas that may result in the development of appropriate biological dosimeters for operational and emergency needs at Chalk River

  13. Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L

    Directory of Open Access Journals (Sweden)

    Yusraini Dian Inayati Siregar

    2017-03-01

    Full Text Available Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektrofotometri. Hasil ekstrasi optimum menggunakan metode maserasi dengan pelarut air  adalah pada temperatur 90°C dan dengan pelarut etanol pada konsentrasi 96 %.Uji stabilitas warna memberikan hasil sebagai berikut: a Kondisi penyimpanan, sinar matahari dan sinar lampu dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan meningkatnya nilai absorbansi pada kedua ekstrak. b Penambahan oksidator, H2O2 dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan dari ekstrak berwarna menjadi ekstrak tidak berwarna karena menghasilkan turunan asam benzoat. c Nilai pH yang semakin meningkat, dari pH 4 ke pH 5, mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan ekstrak berwarna menjadi tidak berwarna karena terbentuknya basa kuinodal.   Kata Kunci: Ekstraksi, Hibiscus rosa-sinensis L, Hibiscus sabdariffa L, Spektrofotometri UV-Vis

  14. An overview of the HIBISCUS campaign

    Science.gov (United States)

    Pommereau, J.-P.; Garnier, A.; Held, G.; Gomes, A. M.; Goutail, F.; Durry, G.; Borchi, F.; Hauchecorne, A.; Montoux, N.; Cocquerez, P.; Letrenne, G.; Vial, F.; Hertzog, A.; Legras, B.; Pisso, I.; Pyle, J. A.; Harris, N. R. P.; Jones, R. L.; Robinson, A. D.; Hansford, G.; Eden, L.; Gardiner, T.; Swann, N.; Knudsen, B.; Larsen, N.; Nielsen, J. K.; Christensen, T.; Cairo, F.; Fierli, F.; Pirre, M.; Marécal, V.; Huret, N.; Rivière, E. D.; Coe, H.; Grosvenor, D.; Edvarsen, K.; di Donfrancesco, G.; Ricaud, P.; Berthelier, J.-J.; Godefroy, M.; Seran, E.; Longo, K.; Freitas, S.

    2011-03-01

    The EU HIBISCUS project consisted of a series of field campaigns during the intense convective summers in 2001, 2003 and 2004 in the State of São Paulo in Brazil. Its objective was to investigate the impact of deep convection on the Tropical Tropopause Layer (TTL) and the lower stratosphere by providing a new set of observational data on meteorology, tracers of horizontal and vertical transport, water vapour, clouds, and chemistry in the tropical Upper Troposphere/Lower Stratosphere (UT/LS). This was achieved using short duration research balloons to study local phenomena associated with convection over land, and long-duration balloons circumnavigating the globe to study the contrast between land and oceans. Analyses of observations of short-lived tracers, ozone and ice particles show strong episodic local updraughts of cold air across the lapse rate tropopause up to 18 or 19 km (420-440 K) in the lower stratosphere by overshooting towers. The long duration balloon and satellite measurements reveal a contrast between the composition of the lower stratosphere over land and oceanic areas, suggesting significant global impact of such events. The overshoots are shown to be well captured by non-hydrostatic meso-scale Cloud Resolving Models indicating vertical velocities of 50-60 m s-1 at the top of the Neutral Buoyancy Level (NBL) at around 14 km, but, in contrast, are poorly represented by global Chemistry-Transport Models (CTM) forced by Numerical Weather Forecast Models (NWP) underestimating the overshooting process. Finally, the data collected by the HIBISCUS balloons have allowed a thorough evaluation of temperature NWP analyses and reanalyses, as well as satellite ozone, nitrogen oxide, water vapour and bromine oxide measurements in the tropics.

  15. The role of bioindicators in assessing radioactive contamination

    International Nuclear Information System (INIS)

    Marovicj, G.

    1990-01-01

    The paper is a survey of investigations into radioactive contamination of selected plant and animal species (bioindicators) which have the capacitr for multiple accumulation of fission products. Literature data on the contamination of bioindicators are compared with special reference to the accumulation of 131 I, 137 Cs and 90 Sr as a result of atmospheric nuclear experiments and the nuclear accident at Chernobyl. (author) 52 refs.; 3 figs [sh

  16. Crocodilians and other reptiles: bioindicators of pollution

    International Nuclear Information System (INIS)

    Manolis, S.C.; Webb, G.J.; Britton, A.R.

    2002-01-01

    The detrimental effects of environmental contamination and pollution (e.g. heavy metals, organochlorines, radionuclides) on wildlife are generally not well known or understood. Research is providing baseline information for various groups of animals, usually because of their sensitivity to changes in their environment (e.g. fish, amphibians), but also where there is a potential conservation threat (e.g. marine mammals). Little research has been directed at reptiles, which may be good bioindicators of their environment. Crocodilians in particular, because of their position in the food chain, aquatic habits and longevity (generally >50 years; Webb and Manolis 1989) may reflect changes in an area over longer periods (Burger et al., 2000). Radioactive contaminants are not generally encountered in crocodilian populations. Radiocesium levels in American alligators living near a reservoir receiving radionuclide-contaminated water from a nuclear reactor were quantified by Brisbin (1989); levels were lower than in various prey items (e.g. fish, waterbirds). This paper provides a brief overview on environmental contamination and reptiles, with particular reference to crocodilians

  17. Microorganisms as bioindicators of pollutants in soil

    Directory of Open Access Journals (Sweden)

    Milošević Nada

    2010-01-01

    Full Text Available Microorganisms are the predominant portion of the soil's biological phase and they are indicators of soil health and quality. Soil microorganisms a take part in degradation of organic and inorganic compounds, b their activity, number and diversity may serve as bioindicators of toxic effects on soil biological activity, c some microbial species may be used for soil bioremediation and d some sensitive microbes are used in eco-toxicity tests. The primary microbial population starts to decompose herbicides several days after their arrival into the soil. The secondary population produces induced enzymes and decomposes herbicides after a period of adaptation. Certain microbial groups are indifferent to the applied herbicides. Effect of heavy metals on soil microbial activity depends on the element, their concentration, microbial species, as well as physical and chemical soil properties. Toxic level of individual pollutants depends on their origin and composition. However, combined application of chemicals makes room for the occurrence of synergistic toxic effects detrimental for the ecosystem and human health. .

  18. Interspecific hybridization among cultivars of hardy Hibiscus species section Muenchhusia

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna; Lütken, Henrik Vlk; Christensen, Brian

    2016-01-01

    Rose mallows belong to the Muenchhusia section of the Hibiscus genus. They represent a small group of cold tolerant North American plants and are popular ornamentals mainly because of their abundant, large and colorful flowers. Due to their geographical origin they are well suited for garden use...... in temperate regions worldwide. The aim of the study was to investigate hybridization barriers in crosses among cultivars of Hibiscus species from the Muenchhusia section: H. coccineus, H. laevis and H. moscheutos. Crossing barriers were identified as both pre- and post-zygotic. The analysis of pollen tube...

  19. MARKS OF ETHNICITY IN PURPLE HIBISCUS TRANSLATION

    Directory of Open Access Journals (Sweden)

    Fernanda de Oliveira Müller

    2016-10-01

    Full Text Available This study gives an analysis of the English – Brazilian Portuguese translation of Chimamanda Ngozi Adichie’s novel, Purple Hibiscus, made by Julia Romeu. It is an attempt to analyze how traces of ethnic identities marked in the source text are reproduced in the Brazilian version Hibisco Roxo, published in 2011. Initially, is a brief biography of the writer is presented together with her history towards the construction of a new paradigm for the literature about Africa and Nigeria. Adichie challenges Western stereotypes about that continent, which tend to report poverty, war and disease scenarios. Secondly, a summary of the story was made and the main characters were described. Thirdly, a collection of recorded words and phrases in the Igbo language was compiled from the original text and an analysis of the translation of those terms into Brazilian Portuguese was performed. Afterwards, the concept of ethnicity described by the sociologist Anthony Giddens was presented. Based on that concept, it was concluded that the terms previously selected could be considered as marks of ethnicity, reflecting the presence of the Igbo ethnic group in the British colonial culture. Finally, taking Antoine Berman’s proposition for an ethical translation, which embraces the foreign and rejects ethnocentrism, the conclusion to be drawn is that the translator’s option to keep Igbo terms in her work respected the author’s manifest intention of, through her work, showing the readers from other countries a bit of Nigeria’s culture and history.

  20. Effect of aqueous extracts of Hibiscus sabdariffa and Zingiber ...

    African Journals Online (AJOL)

    Effect of aqueous extracts of Hibiscus sabdariffa and Zingiber Officinale on blood cholesterol and glucose levels of rats. ... Subsequently, group 3 rats were given 0.8 ml/kg body weight of H. sabdarffa extract, while those in group 4 were given 0.2 ml Z. officinale extract/kg body weight. Rats in group 5 were given 1 ml of H.

  1. Chemical evaluation of three varieties of Roselle ( Hibiscus ...

    African Journals Online (AJOL)

    Chemical evaluation of three varieties of Roselle ( Hibiscus Sabdariffa L.) seeds. SO Babalola, OC Oworh, KO Alasiri, JA Adetunji. Abstract. No Abstract. Nigerian Journal of Horticultural Science Vol. 11 () 2006: pp.31-35. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  2. Haematological effects of aqueous extract of Hibiscus Sabdariffa

    African Journals Online (AJOL)

    The present study was undertaken to investigate the effects of aqueous extract of Hibiscus sabdariffa petals on some haematological parameters. The extract was administered orally in doses of 0.5, 1.0 and 1.5mg/kg body weight to normal Wistar rats for 21 days. The results showed that the extract decreased significantly (p ...

  3. Estimation of Planting Dates for Roselle ( Hibiscus sabdariffa L.) in ...

    African Journals Online (AJOL)

    In order to fill the gap existing between the production and consumption of roselle in the humid tropical areas of Nigeria, this experiment was carried out in Owerri between May and December, 2012 to evaluate the effects of planting dates and accessions on the growth and performance of roselle (Hibiscus sabdariffa).

  4. Comparative water relation of three varieties of Hibiscus cannabinus ...

    African Journals Online (AJOL)

    Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop that is being grown increasingly in tropical and subtropical areas. Choosing the proper variety with the most growth rate and biomass content is a critical point for successful commercial cultivation of kenaf. Since growth and biomass production of plants are strictly ...

  5. Changes in the quality of zobo beverages produced from Hibiscus ...

    African Journals Online (AJOL)

    Quality changes in zobo beverage produced from Hibiscus sabdarifa during storage and the effects of extracts of ginger (Zingiber officinale) alone or in combination with refrigeration at 6C were evaluated for six (6) weeks duration. Results shows that the total viable bacterial count increased from 0.9 ± 0.01 log10 cfu/ml to ...

  6. Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers.

    Science.gov (United States)

    Grajeda-Iglesias, Claudia; Figueroa-Espinoza, Maria C; Barouh, Nathalie; Baréa, Bruno; Fernandes, Ana; de Freitas, Victor; Salas, Erika

    2016-07-22

    The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid-base equilibrium (pK'a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.

  7. Study of the hibiscus esculentus mucilage coagulation–flocculation ...

    African Journals Online (AJOL)

    The flocculent activity of Hibiscus esculentus (gombo) mucilage traditionally used for a local beer (Tchapalo) clarification in Côte d\\'Ivoire was studied using the method of the experimental designs. Of the three factors selected that are the volume of mucilage (X1), the temperature (X2) and the pH (X3), sole X1 and X3 ...

  8. Effects of aqueous extract of Hibiscus sabdariffa calyces on ...

    African Journals Online (AJOL)

    The effect of aqueous extract of Hibiscus sabdariffa calyces on the haematological profile of normal male albino rats was investigated for 28 days using standard methods. The rats were divided into five groups comprising of the control group, I; which received equal volume of distilled water and four treatment groups, II, III, ...

  9. Oppression and Revolt in Chimamanda Adichie's Purple Hibiscus ...

    African Journals Online (AJOL)

    This paper examines the oppressive practice of Chimamanda Adichie's character – Eugene in Purple Hibiscus on members of his nuclear family. This oppression endangers normal familial relationship as the oppressed view their oppression as an infringement on their fundamental human rights. Therefore, they express ...

  10. Haematinic activity of Hibiscus Cannabinus | Agbor | African Journal ...

    African Journals Online (AJOL)

    The haematinic activity of an orally administered aqueous extract of Hibiscus cannabinus leaves was studied on haemolytic anaemic rats. Anaemia was induced by an oral administration of phenylhydrazine for a period of 8 days. Red blood cell count, haemoglobin concentration, and pack cell volume were analysed as ...

  11. Seed trichome variation in Hibiscus section Furcaria in East Africa ...

    African Journals Online (AJOL)

    The micromorphology of the seed trichomes in Hibiscus section Furcaria in Tropical East Africa was surveyed. The structure of the trichomes was found to be unique for each species examined, where such trichomes are present. Seed trichomes of specimens of H. altissimus Horny from the Kenya and Tanzania coasts were ...

  12. Protective influence of Hibiscus sabdariffa , an edible medicinal ...

    African Journals Online (AJOL)

    The present study was undertaken to examine the protective influence of the alcoholic leaf extract of Hibiscus sabdariffa (Linn) Malvaceae (an indigenous edible medicinal plant used in Ayurvedic and traditional Medicine in India, China and Thailand) on oxidative stress during ammonium chloride induced ...

  13. Protective role of aqueous extract of Hibiscus sabdariffa (calyx ...

    African Journals Online (AJOL)

    USER

    2010-05-24

    May 24, 2010 ... The protective role of aqueous extract of Hibiscus sabdariffa (calyx) against potassium bromate induced tissue damage was investigated in rat tissues (brain, kidney, stomach, spleen, heart and liver). The rats were divided into ... diethylnitrosamine in the liver (Tanaka et al., 1993), 4- nitroquinone-1-oxide in ...

  14. Anthocyanin content of two hibiscus sabdariffa cultivars grown

    African Journals Online (AJOL)

    Sukwattanasinit T., Burana-osot J., Sotanaphun. U. 2007. Spectrophotometric Method for. Quantitative Determination of Total. Anthocyanins and Quality Characteristics of Roselle (Hibiscus sabdariffa). Planta Med. 73 (14) : 1517 - 1522. Torres-Morán M.I., Escoto-Delgadillo M., Ron-Parra. J., Parra-Tovar G., Mena-Munguía ...

  15. Protective role of aqueous extract of Hibiscus sabdariffa (calyx ...

    African Journals Online (AJOL)

    The protective role of aqueous extract of Hibiscus sabdariffa (calyx) against potassium bromate induced tissue damage was investigated in rat tissues (brain, kidney, stomach, spleen, heart and liver). The rats were divided into four groups. Group A was administered with 0.25 M sucrose only (base line control), Group B with ...

  16. Toxicity And Immunomodulatory Activity Of Fractions Of Hibiscus ...

    African Journals Online (AJOL)

    This study evaluated immunomodulatory properties and the sub-acute toxicity profile of two fractions of the aqueous alcoholic extract of the dried calyx of Hibiscus sabdariffa in experimental animals. Immunomodulatory activity was evaluated using red blood cell-induced immunostimulation. The fractions were not found to ...

  17. Studies on the constituents of hibiscus rosa-sinensis

    International Nuclear Information System (INIS)

    Hossain, M.A.; Tarafdar, S.A.

    2003-01-01

    A new flavone derivative, furano flavone (1) was isolated along with ovalichalcone (2) from the leaves and stems of Hibiscus rosa-sinensis (Malvaceae). The structure of furanoflavone (1) was identified as 5-methoxy-3' methyl-3', 4'-methylenedioxyfurano(2,3; 7,8) flavone by spectroscopic and chemical analysis. (author)

  18. Membrane Stabilizing Activity And Phytochemistry Of Hibiscus rosa ...

    African Journals Online (AJOL)

    The human erythrocyte membrane stabilizing activity of saline extract of Hibiscus rosa-sinensis leaves was investigated as part of efforts at validating its use as anti-arthritic and anti-inflammatory agent. The results of the membrane stabilizing activity of the extract, when compared to two non-steroidal anti-inflammatory drugs ...

  19. Effect of aqueous extract of Hibiscus sabdariffa Calyces on anaemia ...

    African Journals Online (AJOL)

    The effect of aqueous extract of Hibiscus sabdariffa Calyces on anaemia-induced and normal wistar albino rats were investigated using standard protocols. The haematological parameters which include haemoglobin concentration, packed cell volume, erythrocyte sedimentation rate, Red Blood Cell count and White Blood ...

  20. The effect of aqueous extracts of Hibiscus sabdariffa (Sorrel) calyces ...

    African Journals Online (AJOL)

    The effect of aqueous extracts of Hibiscus sabdariffa (Sorrel) calyces on heamatological profile and organ pathological changes in Trypanasoma congolense – infected ... Consumption of the extract significantly (p<0.01) retarded the rate of weight gain in both healthy and infected rats; even though the feed-intake was not ...

  1. Effect of Hibiscus sabdariffa on Blood Pressure and Electrolyte ...

    African Journals Online (AJOL)

    Effect of Hibiscus sabdariffa on Blood Pressure and Electrolyte Profile of Mild to Moderate Hypertensive Nigerians: A Comparative Study with Hydrochlorothiazide. ... Aim: The aim of this study is to investigate the effect of HS consumption on blood pressure (BP) and electrolytes of mild to moderate hypertensive Nigerians ...

  2. Anthocyanin content of two Hibiscus sabdariffa cultivars grown in ...

    African Journals Online (AJOL)

    Anthocyanin content of Hibiscus sabdariffa calyces was determined to compare two cultivars from Senegal called Koor and Vimto. Results showed a significant difference in terms of total anthocyanin content (TA) and relative abundance (RA) of anthocyanin species. Values of TA for Vimto were 3-fold higher than Koor's.

  3. Utilization of Ocimum gratissimum and the calyx of Hibiscus ...

    African Journals Online (AJOL)

    This preliminary study was carried out to evaluate the utilization of Ocimum gratissimum and the calyx of Hibiscus sabdariffa leaf meal in starter diets by broiler chicks, for a total period of 42 days between May-June 2011. Eighty (80) broiler chicks brooded together for 7 days with a mean body weight of 97gm were randomly ...

  4. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Nx 6110

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, an.

  5. Lawsonia inermis And Hibiscus sabdariffa : Posible Histological Stains

    African Journals Online (AJOL)

    The ability of various concentrations of aqueous extracts of Lawsonia inermis and Hibiscus sabdariffa to stain histological tissues was demonstrated. The results with sections of tongue and kidney of the laboratory rat, cut at 6microns thickness showed that only the cellular cytoplasm was stained. However, combinations of ...

  6. Indigenous Tea Production From Calyces Of Hibiscus Sabdariffa L ...

    African Journals Online (AJOL)

    Studies were carried out on the possibility of producing an acceptable indigenous tea from a commonly available indigenous raw material, Hibiscus sabdariffa L. The said tea was blended with ground dried leaves of Cymbopogon citratus and ground dried peels of Citrus limon. The commonly consumed commercial green ...

  7. Comparative water relation of three varieties of Hibiscus cannabinus ...

    African Journals Online (AJOL)

    Yomi

    2011-10-17

    Oct 17, 2011 ... biochemistry (Tanner and Sinclair, 1983; Hsiao,. 1973;Foster, 1992; Knapp et al., 1993; Schulze and Hall,. 1982; Jackson et .... days old Hibiscus cannabinus varieties; G4, V36 and. KK60. The F-statistics for ANOVA models comparing varieties differences in each characteristic are presented. Characteristic.

  8. Effect of Hibiscus sabdariffa calyx extract on carbon tetrachloride ...

    African Journals Online (AJOL)

    The effect of aqueous ethanol (1:1) extract of the calyx of Hibiscus sabdariffa on carbon tetrachloride (CCl4) induced liver damage was investigated. Oral administration of the extract following a single CCl4 dose promoted the healing of oxidative liver damage as determined by serum aminotransferases, ALT, AST, levels ...

  9. Verification of the folkloric diuretic claim of Hibiscus sabdariffa L ...

    African Journals Online (AJOL)

    The folklorically acclaimed diuretic activity of the petal extract of Hibiscus sabdariffa was verified in saline-loaded albino rats (80 – 220 g; n=5) according to the method of De la Peurta Vazquez et al, 1989. A methanolic extract of the dried petals was prepared, and, upon lethal toxicity testing, was found to be very safe – LD50 ...

  10. Antibacterial activity of Hibiscus sabdariffa , Acacia seyal var. seyal ...

    African Journals Online (AJOL)

    Introduction: Medicinal plants constitute an effective source of both traditional and modern medicines. The emergence of drug resistant bacteria mandates the need for newer antibiotics. Objective: To evaluate the antibiotic effect of Hibiscus sabdariffa. Methods: Three medicinal plants have been selected for investigation of ...

  11. Effect of aqueous extracts of Hibiscus sabdariffa and Zingiber ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-11-05

    Nov 5, 2008 ... 1Department of physiology, School of Basic Medical Sciences, University of Benin, Nigeria. ... Cholesterol is the major sterol in the human body. It is a structural .... (9th edition), pp. 239-240. Herunsalee J, Suthisisang C (2005). Antioxidant effect aqueous extracts from dried calyx of Hibiscus sabdariffa Linn.

  12. Domestic violence in Chimamanda Ngozi Adichie's Purple Hibiscus ...

    African Journals Online (AJOL)

    Gender-based violence is an issue that has become a part of modern society, cutting across cultures, race, ethnicity and status. In Adichie's Purple Hibiscus, gender-based violence manifests in the form of domestic violence, projected through the Eugene Achike family around whom the story, set in the eastern part of ...

  13. Microbial contaminants of cultured Hibiscus cannabinus and Telfaria ...

    African Journals Online (AJOL)

    Nine microbial contaminants comprising of five bacteria and four fungi species were isolated from Hibiscus cannabinus and Telfaria occidentalis cultured tissues. The rate of occurrence of bacteria isolates was higher than that of fungi. The bacterial isolates includes Pseudomonas syringae pv phaseolicoli, Bacillus ...

  14. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, ...

  15. Protective role of aqueous extract of Hibiscus sabdariffa (calyx ...

    African Journals Online (AJOL)

    USER

    2010-05-24

    May 24, 2010 ... of electron on the electron transport chain. There is a dose-dependent relationship between lipid peroxidation induced by potassium bromate and the protection offered by the extract of H. sabdariffa. REFERENCES. Alli MB, Salih M (1991). Investigation of the antispasmodic potential of. Hibiscus sabdariffa ...

  16. Production of red wine from roselle (Hibiscus sabdariffa) and ...

    African Journals Online (AJOL)

    Red wine was produced from must formulated by mixing 10% (w/v) roselle calyces (Hibiscus sabdriffa var. sabdariffa) Hot water extract with pawpaw juice at the rate of 3:1. The must was formulated using a pure strain of Sacchromyces cerevisiae isolated from palm wine, propagated and pitched at a rate of 8% (v/v).

  17. The Aqueous Calyx Extract of Hibiscus sabdariffa Lowers Blood ...

    African Journals Online (AJOL)

    Summary: The antihypertensive effect of Hibiscus sabdariffa (HS) has been validated in animals and man. This study tested the hypothesis that its hypotensive effect may be sympathetically mediated. The cold pressor test (CPT) and handgrip exercise (HGE) were performed in 20 healthy subjects before and after the oral ...

  18. Women's Struggles and Independence in Adichie's Purple Hibiscus ...

    African Journals Online (AJOL)

    Love, war, conflict and the persistent inequality between men and women are among the dominant themes in Adichie's Purple Hibiscus and Half of a Yellow Sun. Adichie, however, projects womanhood in a positive light. She upholds female potentialities which the patriarchal structure has repressed. She also makes role ...

  19. Adichie's Purple Hibiscus and issues of ideology in the constitution ...

    African Journals Online (AJOL)

    ... as subjects, with the eventual emergence of Kambili to self-knowledge and condition of social responsibility. The aim of this is to examine how Adichie's Purple Hibiscus is typical of how the Nigerian novel engages itself in issues of ideology and how these issues, in turn, crystallize the challenges of nation-ness in Nigeria.

  20. Fermentation potentials of Citrus limon and Hibiscus sabdariffa juice ...

    African Journals Online (AJOL)

    This study aimed to determine the fermentation potentials of yeast isolates from Citrus limon and Hibiscus sabdariffa for the fermentation of juice extracts of C. limon and H. sabdariffa. Isolation and morphological studies of yeast cells were carried out by standard protocols. Fourier Transform Infra-red (FT-IR) ...

  1. Relationships between anthocyanins and other compounds and sensory acceptability of Hibiscus drinks.

    Science.gov (United States)

    Bechoff, Aurélie; Cissé, Mady; Fliedel, Geneviève; Declemy, Anne-Laure; Ayessou, Nicolas; Akissoe, Noel; Touré, Cheikh; Bennett, Ben; Pintado, Manuela; Pallet, Dominique; Tomlins, Keith I

    2014-04-01

    Chemical composition of Hibiscus drinks (Koor and Vimto varieties, commercial and traditional, infusions and syrups) (n=8) was related to sensory evaluation and acceptance. Significant correlations between chemical composition and sensory perception of drinks were found (i.e. anthocyanin content and Hibiscus taste) (pHibiscus drink and anthocyanin content. The study showed that the distinctions between the acceptability groups are very clear with respect to the chemical composition and rating of sensory attributes. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Leaves of roadside plants as bioindicator of traffic related lead ...

    African Journals Online (AJOL)

    JUDITH

    Plants are important bioindicators of heavy metal environmental pollution. For this purpose a study was conducted during winter and summer seasons in Sargodha, Pakistan. Three roadside plants that is. Dalbergia sissoo Roxb., Prosopis juliflora L. and Eucalyptus spp. were collected from different locations. The study area ...

  3. Bioindicator demonstrates high persistence of sulfentrazone in dry soil

    Directory of Open Access Journals (Sweden)

    Renato Coradello Lourenço

    2015-09-01

    Full Text Available In sugarcane crop areas, the application of preemergence herbicides with long residual effect in the soil has been frequently necessary. The herbicide persistence in the soil must be high especially because of applications during the dry season of the year, after sugarcane harvest. This study aimed at estimating the sulfentrazone persistence and dissipation in dry soil using bioindicator. Five experiments were carried out, divided into two phases. In the first phase, three dose-response curves were adjusted to select the best bioindicator to be adopted in the second phase. Niger was adopted due to its lower sensibility to sulfentrazone. In the second phase, a new dose-response curve was carried out, with six doses of sulfentrazone, in order to standardize the bioindicator sensibility to sulfentrazone. At the end, another experiment with six periods of sulfentrazone persistence in dry clay soil was developed. Persistence periods were: 182, 154, 125, 98 and 30 days. The bioindicator was seeded at the application day in treated plots and control. In this experiment, the sulfentrazone dose applied was 800 g ha-1. Niger was considered a good species to estimate the sulfentrazone persistence in dry soil. The sulfentrazone phytotoxic activity was identified up to 182 days after application, and its average dissipation rate was 2.15 g ha-1 day-1, with half-life higher than 182 days.

  4. Macroinvertebrates as bioindicators of water quality in the Mkondoa ...

    African Journals Online (AJOL)

    The suitability of using macroinvertebrates as bioindicators of stream water quality was tested in the Mkondoa River in an agricultural area at Kilosa, using the rapid bioassessment protocol. The family biotic index (FBI) showed marked variation in water quality along the stream from values ranging from 4.1 to 5.0 in the ...

  5. An assessment of pollution in aquatic environment using bioindicators

    African Journals Online (AJOL)

    This review highlights the importance of biological indicators in monitoring presence of pollution in aquatic environment. This assessment involves the use of living organisms (macro or microorganisms and plants or animals) as bioindicators of pollution in water bodies. These organisms are believed to show higher ...

  6. Assessment of Brown Bear\\'s (Ursus arctos syriacus Winter Habitat Using Geographically Weighted Regression and Generalized Linear Model in South of Iran

    Directory of Open Access Journals (Sweden)

    A. A. Zarei

    2016-03-01

    Full Text Available Winter dens are one of the important components of brown bear's (Ursus arctos syriacus habitat, affecting their reproduction and survival. Therefore identification of factors affecting the habitat selection and suitable denning areas in the conservation of our largest carnivore is necessary. We used Geographically Weighted Logistic Regression (GWLR and Generalized Linear Model (GLM for modeling suitability of denning habitat in Kouhkhom region in Fars province. In the present research, 20 dens (presence locations and 20 caves where signs of bear were not found (absence locations were used as dependent variables and six environmental factors were used for each location as independent variables. The results of GLM showed that variables of distance to settlements, altitude, and distance to water were the most important parameters affecting suitability of the brown bear's denning habitat. The results of GWLR showed the significant local variations in the relationship between occurrence of brown bear dens and the variable of distance to settlements. Based on the results of both models, suitable habitats for denning of the species are impassable areas in the mountains and inaccessible for humans.

  7. Hibiscus sabdariffa L., roselle calyx, from ethnobotany to pharmacology

    Science.gov (United States)

    Carvajal-Zarrabal, Octavio; Barradas-Dermitz, Dulce María; Orta-Flores, Zaida; Hayward-Jones, Patricia Margaret; Nolasco-Hipólito, Cirilo; Aguilar-Uscanga, M Guadalupe; Miranda-Medina, Anilú; Bujang, Kopli Bin

    2012-01-01

    Using MEDLINE and SCOPUS databases, a review of the literature from the pioneering study of 1991 until 2010 was performed on the effects on biological models of Hibiscus sabdariffa L. roselle calyx, its extracts mainly in polar solvents, or pure components found in extracts, as well as their possible relationship to these effects. Three relevant effects on lipid metabolism, antihypertensive activity, and apoptosis were observed. Our chronological review of the studies mentioned in the literature provides another opportunity to see how humans compile scientific knowledge of a chemical structure–physiological activity relationship starting from an ethnobotanical–ethnopharmagognosy contribution. The chemical components that are the main active principles in the physiological activities of Hibiscus sabdariffa L. calyx are anthocyanins and polyphenols (protocatechuic acid and quercetin). Advances have also been made in the elucidation of action mechanisms. Additionally, it has become clear that the lack of standardization in terms of chemical components of the material arising from Hibiscus sabdariffa L. used in testing on biological models imposes limits on the possibility of carrying out comparative analyses between studies. Fortunately, more recent studies are overcoming this obstacle by reporting component concentrations of assumed active principles; however, complete analysis of the extract, if this is to be considered as a therapeutic agent, is not commonly reported in the aforesaid studies. If one of the eventual scenarios for Hibiscus sabdariffa L. calyx is as a therapeutic agent in communities with economic limitations, then studies of a pharmacological nature should guarantee the effectiveness, safety, and tolerability of this material, which is widely accepted to be associated with chemical complexity, thus making this knowledge necessary. PMID:27186114

  8. Kombucha Rosella (Hibiscus Sabdariffa Linn) dan Kemampuannya sebagai Antihiperkolesterolemia

    OpenAIRE

    Suhartatik, Nanik; Karyantina, Merkuria; Purwanti, Indrias Tri

    2009-01-01

    Kita mengenal adanya teh yang diekstrak dari kelopak bunga rosella (Hibiscus sabdariffa Linn). Teh jenis ini dapat dibuat menjadi kombucha dengan cara memfermentasikan ekstrak rosella menggunakan peran mikrobia dalam fer­ mentasi kombucha. Mikrobia dalam fermentasi kombucha ini ditumbuhkan dalam ekstrak rosella sebagai medium per­ tumbuhan dengan variasi konsentrasi rosella (30, 40, 50 gram rosella kering/L). Selama proses fermentasi, kombucha rosella dianalisis pH, total asam, dan aktivitas ...

  9. Investigation on the phytosanitary status of major ornamental hibiscus species in Italy to assess virus infection

    Science.gov (United States)

    The genus Hibiscus (family Malvaceae) includes more than 250 species that vary from annual to perennial herbs, and shrubs to small trees that are native to tropical, sub-tropical and temperate climates. A study in 2010-2011 examined viruses associated with symptoms observed on hibiscus plants in It...

  10. Two New Disease-Resistant Hibiscus: 'Lufkin Red' and 'Lufkin White'

    Science.gov (United States)

    The Agricultural Research Service, United States Department of Agriculture, has released Hibiscus laevis ‘Lufkin Red’ and ‘Lufkin White’, two new hardy native ornamental hibiscus. These cultivars are recommended for trial by gardeners and horticulturists as summer flowering perennial landscape pl...

  11. In vitro inhibitory activities of the extract of Hibiscus sabdariffa L ...

    African Journals Online (AJOL)

    In vitro inhibitory activities of the extract of Hibiscus sabdariffa L. (family malvaceae) on selected cytochrome p450 isoforms. SS Johnson, FT Oyelola, T Ari, H Juho. Abstract. Literature is scanty on the interaction potential of Hibiscus sabdariffa L., plant extract with other drugs and the affected targets. This study was ...

  12. An artificial bioindicator system for network intrusion detection.

    Science.gov (United States)

    Blum, Christian; Lozano, José A; Davidson, Pedro Pinacho

    2015-01-01

    An artificial bioindicator system is developed in order to solve a network intrusion detection problem. The system, inspired by an ecological approach to biological immune systems, evolves a population of agents that learn to survive in their environment. An adaptation process allows the transformation of the agent population into a bioindicator that is capable of reacting to system anomalies. Two characteristics stand out in our proposal. On the one hand, it is able to discover new, previously unseen attacks, and on the other hand, contrary to most of the existing systems for network intrusion detection, it does not need any previous training. We experimentally compare our proposal with three state-of-the-art algorithms and show that it outperforms the competing approaches on widely used benchmark data.

  13. Phytoplankton community as bioindicator of fertility in belawan river

    Science.gov (United States)

    Sari Yeanny, Mayang

    2018-03-01

    Belawan River is an important river for the Medan residents and its surroundings. It serves as the main raw material for the local drinking water company, as well as domestic, industrial, hotel and tourism. Many human activities had led to the declining condition of water in the river throughout the year. One way to approach the concept of bioindicator is by knowing Abundance, Relative Abundance, Frequency of Attendance, equitability, dominance, and diversity of the phytoplankton itself. Results indicated that the phytoplankton community was from 3 different classes: Chlorophyceae, Bacillariophyceae, and Cyanophyceae. Phytoplankton individual abundance was around 2612 to 17755 ind / L. The diversity index was around 2.15 to 2.58, which is considered to have low to moderate diversity with high pollution level. Equitability Index was approaching 0, with relatively high domination from Sphaeroplea and Asterionella. The water quality that influences the diversity of phytoplankton as bioindicator was dissolved oxygen.

  14. Relative Condition Factors of Fish as Bioindicators One Year after the Deepwater Horizon Oil Spill

    Science.gov (United States)

    2012-02-01

    Research Report 3. DATES COVERED (From - To) 07-01-2010 to 30-1-2012 4. TITLE AND SUBTITLE Relative Condition Factors of Fish as Bioindicators One...Condition index, relative condition factor, bioindicator , oil spill 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF...Condition Factors of Fish as Bioindicators One Year after the Deepwater Horizon Oil Spill Joshua Courtney,1 Taylor Klinkmann,2 Amy Courtney,1 Joseph

  15. Bee pollen as a bioindicator of environmental pesticide contamination.

    Science.gov (United States)

    de Oliveira, Renata Cabrera; Queiroz, Sonia Claudia do Nascimento; da Luz, Cynthia Fernandes Pinto; Porto, Rafael Silveira; Rath, Susanne

    2016-11-01

    Honeybees and bee products are potential bioindicators of the presence of contaminants in the environment, enabling monitoring of large areas due to the long distances travelled by bees. This work evaluates the use of bee pollen as a bioindicator of environmental contamination by pesticides. A GC-MS/MS analytical method for multiresidue determination of 26 different pesticides in pollen was developed and validated in accordance with the recommendations of the European Union SANCO guide. Environmental monitoring was conducted using the analysis of 145 pollen samples collected from ten beehives in the experimental apiary of Embrapa in Jaguariúna (São Paulo State, Brazil). Bioallethrin and pendimethalin were identified in four and eighteen samples, respectively, at concentrations below the LOQ of the method (25 ng g(-1)). Passive sampling with polyurethane foam discs was used as a control, and no pesticides were found. The detection of pesticide residues in seven samples (33%) from commercial apiaries in Ribeirão Preto (São Paulo State) confirmed the efficiency of the analytical method and the need for environmental monitoring for the presence of pesticide residues. The results demonstrated the potential of bee pollen as a bioindicator of environmental contamination by pesticides. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Using Ants as bioindicators: Multiscale Issues in Ant Community Ecology

    Directory of Open Access Journals (Sweden)

    Alan Andersen

    1997-06-01

    Full Text Available Ecological patterns and processes are characteristically scale dependent, and research findings often cannot be translated easily from one scale to another. Conservation biology is challenged by a lack of congruence between the spatial scales of ecological research (typically involving small plots and land management (typically involving whole landscapes. Here, I discuss spatial scaling issues as they relate to an understanding of ant communities and, consequently, their use as bioindicators in land management. Our perceptions of fundamental patterns and processes in ant communities depend on scale: taxa that are behaviorally dominant at one scale are not necessarily so at others, functional groups recognized at one scale are often inappropriate for others, and the role of competition in community structure depends on the scale of analysis. Patterns of species richness and composition, and the ability of total richness to be estimated by surrogates, are all also scale dependent. Ant community ecology has a tradition of detailed studies in small plots, but the use of ants as bioindicators requires a predictive understanding of community structure and dynamics at a range of spatial scales. Such an appreciation of ant communities and their most effective use as bioindicators is best served by studies integrating results from plot-scale research with the broad-scale paradigms of biogeography, systematics, and evolutionary biology.

  17. Ekstraksi Dan Uji Stabilitas Zat Warna Alami Dari Bunga Kembang Sepatu (Hibiscus Rosa-sinensis L) Dan Bunga Rosela (Hibiscus Sabdariffa L)

    OpenAIRE

    Siregar, Yusraini Dian Inayati; Nurlela, Nurlela

    2011-01-01

    Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L) dan Bunga Rosela (Hibiscus sabdariffa L) telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektr...

  18. Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L) dan Bunga Rosela (Hibiscus sabdariffa L)

    OpenAIRE

    Yusraini Dian Inayati Siregar; Nurlela Nurlela

    2017-01-01

    Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L) dan Bunga Rosela (Hibiscus sabdariffa L) telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektr...

  19. STUDIES ON HIBISCUS CANNABINUS, HIBISCUS SABDARIFFA, AND CANNABINUS SATIVA PULP TO BE A SUBSTITUTE FOR SOFTWOOD PULP- PART 1: AS-AQ DELIGNIFICATION PROCESS

    OpenAIRE

    Dharm Dutt; J. S. Upadhyaya; C. H. Tyagi

    2010-01-01

    Hibiscus cannabinus, Hibiscus sabdariffa, and Cannabinus sativa, which are renewable non-woody fiber resources having characteristics similar to that of softwood (bast fibers), when used together with hardwood (core fibers), gave higher pulp yield with good mechanical strength properties when using an alkaline sulphite-anthraquinone (AS-AQ) pulping process rather than a conventional kraft pulping process and bleached more readily than kraft and soda pulps with a CEHH bleaching sequence. A com...

  20. Physicochemical and phytochemical properties of cold and hot water extraction from Hibiscus sabdariffa.

    Science.gov (United States)

    Ramirez-Rodrigues, Milena M; Plaza, Maria L; Azeredo, Alberto; Balaban, Murat O; Marshall, Maurice R

    2011-04-01

    Hibiscus cold (25 °C) and hot (90 °C) water extracts were prepared in various time-temperature combinations to determine equivalent extraction conditions regarding their physicochemical and phytochemical properties. Equivalent anthocyanins concentration was obtained at 25 °C for 240 min and 90 °C for 16 min. Total phenolics were better extracted with hot water that also resulted in a higher antioxidant capacity in these extracts. Similar polyphenolic profiles were observed between fresh and dried hibiscus extracts. Hibiscus acid and 2 derivatives were found in all extracts. Hydroxybenzoic acids, caffeoylquinic acids, flavonols, and anthocyanins constituted the polyphenolic compounds identified in hibiscus extracts. Two major anthocyanins were found in both cold and hot extracts: delphynidin-3-sambubioside and cyanidin-3-sambubioside. In general, both cold and hot extractions yielded similar phytochemical properties; however, under cold extraction, color degradation was significantly lower and extraction times were 15-fold longer. Hibiscus beverages are prepared from fresh or dried calyces by a hot extraction and pasteurized, which can change organoleptic, nutritional, and color attributes. Nonthermal technologies such as dense phase carbon dioxide may maintain their fresh-like color, flavor, and nutrients. This research compares the physicochemical and phytochemical changes resulting from a cold and hot extraction of fresh and dried hibiscus calyces and adds to the knowledge of work done on color, quality attributes, and antioxidant capacity of unique tropical products. In addition, the research shows how these changes could lead to alternative nonthermal processes for hibiscus.

  1. Isolation and identification of an allelopathic substance from Hibiscus sabdariffa.

    Science.gov (United States)

    Suwitchayanon, Prapaipit; Pukclai, Piyatida; Ohno, Osamu; Suenaga, Kiyotake; Kato-Noguchi, Hisashi

    2015-05-01

    In this study, an allelopathic substance was isolated from an aqueous methanol extract of Hibiscus sabdariffa L. by column chromatography and reverse phase HPLC. The chemical structure of the substance was determined by 1H NMR spectroscopy and mass spectrometry as trimethyl allo-hydroxycitrate. Trimethyl allo-hydroxycitrate inhibited the growth of cress hypocotyls and roots at concentrations greater than 10 mM. The concentrations required for 50% growth inhibition of the hypocotyls and roots of cress were 20.3 and 14.4 mM, respectively. The inhibitory activity of trimethyl allo-hydroxycitrate suggests that the substance may act as an allelopathic substance of H. sabdariffa.

  2. Kombucha Rosella (Hibiscus sabdariffa Linn) dan Kemampuannya sebagai Antihiperkolesterolemia

    OpenAIRE

    Suhartatik, Nanik; Karyantina, Merkuria; Purwanti, Indrias Tri

    2012-01-01

    We know that there is another tea extract, e.i from calyx of roselle flower (Hibiscus sabdariffa Linn). This kind of tea could be made became kombucha by ferment roselle extract using microbe in the fermentation of kombucha. This mi­ crobe grown in roselle extract as a medium with variety of roselle concentration (30; 40; 50 grams of dried roselle/L). During the fermentation process, roselle kombucha was analyzed for pH value, total acid, antioxidant activity at 0, 1, 3, 5, 7, 10 days of ferm...

  3. Changes in rate serum bioindicators after single or repeated dosages of fipronil

    Science.gov (United States)

    Acute exposure to different classes of pesticides produces different patterns of changes in serum bioindicators (Herr et al., Toxicologist, 2013). We now examined profiles of bioindicators after 1 or 14 treatments with 0, 5, or 10 mg/kg/day fipronil (po). Adult male Long-Evans ra...

  4. Bioindicators for monitoring radioactive pollution of the marine environment

    International Nuclear Information System (INIS)

    Dahlgaard, H.

    1981-05-01

    Mussels (Mytilus edulis) are globally used as bioindicators for pollution of coastal and estuarine environments by metals and radionuclides. The aim of this work has been to improve the use of Mytilus edulis as a bioindicator by gaining knowledge on its accumulation and loss of certain radionuclides ( 65 Zn, 57 Co, 54 Mn, 51 Cr, 59 Fe and 134 Cs) under different fieldcomparable environmental conditions. A laboratory set-up in which natural concentrations of suspended phytoplankton are kept constant for weeks was evolved for the accumulation experiments with mussels. It is argued that continuous feeding at very low (natural) levels is necessary if field-comparable experiments are to be performed with suspension feeding bivalves. Accumulation via food intake was studied by comparing experiments with different concentrations of contaminated phytoplankton (Phaeodactylum tricornutum). This comparison showed no effect of varying the phytoplankton concentration. Decreasing the salinity and increasing the temperature elevated the influx (initial rate of accumulation) of the radionuclides. During one year excretion experiments were performed by weekly wholebody countings of laboratory contaminated mussels which had been re-introduced in their natural environment. A seasonal effect on the biological half life was detected for 65 Zn. It is concluded that mussels are useful bioindicators provided the variability due to environmental factors, e.g. season and salinity, is taken into consideration. Brown algae, expecially Fucus vesiculosus, were used to trace the controlled liquid discharges (mainly 60 Co, 58 Co, 65 Zn, 54 Mn and sup(110m)Ag) from two Swedish nuclear power plants (Barsebaeck and Ringhals)> Fucus showed higher accumulation than Mytilus. Transfer factors between discharge and sample from a specified location are presented. It is argued that these transfer factors may be useful in estimating the magnitude of an uncontrolled accidental release of activity and its

  5. Biofilms as bioindicators of mercury exposure in surface waters

    Science.gov (United States)

    Le Faucheur, S.; Dranguet, P.; Freiburghaus, A.; Slaveykova, V. I.; Carmen, M.

    2016-12-01

    Biofilms are communities of microorganisms living in shallow waters, which possess several interesting characteristics to be a good bioindicator, i.e. ubiquitous, easy to collect, integrator of the quality of their ambient environment and sensitive to pollutants. Our work investigated whether biofilm could be used as a bioindicator of Hg contamination in natural waters and explore the links between ambient Hg concentrations, its bioaccumulation and biofilm composition. To that end, field- and lab-based experiments were performed with biofilm grown on artificial substrata in either Hg-impacted waters and in microcosms spiked with Hg. The field experiments were performed in contrasted environments: the Olt River (Romania) impacted by chloro-alkali releases and the lagoon of Venice (Italy) affected by urban, industrial and agricultural activities. The field studies were complemented with microcosm experiments using Geneva Lake water enriched with Hg (13 pM to 1.4 nM). Bioaccumulated inorganic HgII and methylmercury (CH3Hg) in biofilms were measured w/o cysteine washing step to assess the total and intracellular Hg content. Biofilm composition was examined from gene to community levels using several techniques, notably qPCR, epifluorescent microscopy and pyrosequencing. In parallel the physico-chemical parameters of the ambient waters (pH, anion/cation and dissolved organic matter concentrations) were well characterized and dissolved Hg concentrations measured. Commercially available DGTs for Hg were additionally used in the lagoon of Venice. The main findings of the study can be summarized as follows: (i) accumulation of Hg in biofilms well correlates with ambient Hg concentration in a specific environment, but (ii) that bioaccumulation highly depends of biofilm composition, with bacterial-dominated biofilms having higher uptake rate constants than algal-dominated biofilms, and (iii) Hg exposure at environmental low concentrations (pM levels) strongly impacts algal

  6. [Bioindications for elevated metal contents in flowing waters].

    Science.gov (United States)

    Wachs, B

    1983-12-01

    Lawful arguments of food research require an information of the concentrations of heavy metals in fishes. Whereas for the control of emissions and of river pollution the analysis on heavy metals in excellent indicator organisms - as specific submerged water plants or fish food (zoobenthic) organisms - is generally more sensitive and therefore more suitable. With analytical results there is no successive accumulation of metals in the aquatic food chain - that means no biomagnification - determinable. By the analysis of some bioindicators the degree of the environmental load due to heavy metals can be estimated. With this ecological method we are able to detect the emissions' locality and their producers.

  7. Hibiscus sabdariffa L. - a phytochemical and pharmacological review.

    Science.gov (United States)

    Da-Costa-Rocha, Inês; Bonnlaender, Bernd; Sievers, Hartwig; Pischel, Ivo; Heinrich, Michael

    2014-12-15

    Hibiscus sabdariffa L. (Hs, roselle; Malvaceae) has been used traditionally as a food, in herbal drinks, in hot and cold beverages, as a flavouring agent in the food industry and as a herbal medicine. In vitro and in vivo studies as well as some clinical trials provide some evidence mostly for phytochemically poorly characterised Hs extracts. Extracts showed antibacterial, anti-oxidant, nephro- and hepato-protective, renal/diuretic effect, effects on lipid metabolism (anti-cholesterol), anti-diabetic and anti-hypertensive effects among others. This might be linked to strong antioxidant activities, inhibition of α-glucosidase and α-amylase, inhibition of angiotensin-converting enzymes (ACE), and direct vaso-relaxant effect or calcium channel modulation. Phenolic acids (esp. protocatechuic acid), organic acid (hydroxycitric acid and hibiscus acid) and anthocyanins (delphinidin-3-sambubioside and cyanidin-3-sambubioside) are likely to contribute to the reported effects. More well designed controlled clinical trials are needed which use phytochemically characterised preparations. Hs has an excellent safety and tolerability record. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  8. Analysis of MINT hibiscus rosa-sinensis mutants

    International Nuclear Information System (INIS)

    Shakinah Salleh; Shuhaimi Shamsudin; Putri Noor Faizah MM Tahir; Mohd Nazir Basiran

    2006-01-01

    Gamma irradiation is an alternative method to create new cultivars of Hibiscus rosa-sinensis in Malaysia. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. The LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three new cultivars with variations in flower size and colours were obtained. The plants were propagated for five generations to achieve the stable mutants. Anthocyanins analysis is being carried out to measure the amount of anthocyanins in each cultivar. Pelargonidin chloride, Cyanidin chloride and Delphinidin chloride are used as standards for the analysis. (Author)

  9. Extraction of total polyphenols from hibiscus (Hibiscus sabdariffa L. and waxweed / ‘sete-sangrias’ (Cuphea carthagenensis and evaluation of their antioxidant potential

    Directory of Open Access Journals (Sweden)

    Daniele Begmeier

    2014-02-01

    Full Text Available Current research investigates the extraction process of total polyphenols from hibiscus (Hibiscus sabdariffa L. and waxweed (Brazilian name: ‘sete-sangrias’ (Cuphea carthagenensis and evaluates the antioxidant potential of their extracts. The extraction stage comprised investigation on the following parameters: i solvents (acetone and ethanol pure and fractioned with water; ii variables (temperature, stirring, solvent ratio, time and pH. Total polyphenols were quantified by Folin-Ciocalteau reagent and antioxidant activity was determined by ABTS•+ (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid and DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Results showed that, depending on experimental conditions, total phenolic contents for hibiscus and waxweed ranged between 460.86 mg GAE 100 g-1 and 5012.54 mg GAE 100 g-1 and between 462.86 mg GAE 100 g-1 and 4215.99 mg GAE 100 g-1, respectively. Waxweed had a higher antioxidant activity when compared to that of hibiscus by both ABTS•+ and DPPH. Data showed that hibiscus and waxweed have a significant amount of polyphenols which may be extracted in mild processing conditions and then employed as natural antioxidant sources in industrial processes.

  10. Hibiscus vitifolius (Linn.) root extracts shows potent protective action against anti-tubercular drug induced hepatotoxicity.

    Science.gov (United States)

    Samuel, Anbu Jeba Sunilson John; Mohan, Syam; Chellappan, Dinesh Kumar; Kalusalingam, Anandarajagopal; Ariamuthu, Saraswathi

    2012-05-07

    The roots of Hibiscus vitifolius Linn. (Malvaceae) is used for the treatment of jaundice in the folklore system of medicine in India. This study is an attempt to evaluate the hepatoprotective activity of the roots of Hibiscus vitifolius against anti-tubercular drug induced hepatotoxicity. Hepatotoxicity was induced in albino rats of either sex by oral administration of a combination of three anti-tubercular drugs. Petroleum ether, chloroform, methanol and aqueous extracts of roots of Hibiscus vitifolius (400mg/kg/day) were evaluated for their possible hepatoprotective potential. All the extracts were found to be safe up to a dose of 2000mg/kg. Among the four extracts studied, oral administration of methanol extract of Hibiscus vitifolius at 400mg/kg showed significant difference in all the parameters when compared to control. There was a significant (PHibiscus vitifolius have potent hepatoprotective activity, thereby justifying its ethnopharmacological claim. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  11. Kenaf (Hibiscus cannabinus) and sunn hemp (Crotalaria juncea): Monoculture and polyculture production

    Science.gov (United States)

    Kenaf (Hibiscus cannabinus L.) and sunn hemp (Crotalaria juncea L.) are fast growing summer annual crops with numerous commercial applications (fibers, biofuels, bioremediation, paper pulp, building materials, cover crops, and livestock forages). Field research was conducted in southeast Oklahoma (...

  12. Pharmacological characterization of the diuretic effect of Hibiscus sabdariffa Linn (Malvaceae) extract.

    Science.gov (United States)

    Alarcón-Alonso, Javier; Zamilpa, Alejandro; Aguilar, Francisco Alarcón; Herrera-Ruiz, Maribel; Tortoriello, Jaime; Jimenez-Ferrer, Enrique

    2012-02-15

    Hibiscus sabdariffa L. (Malvaceae) populary known in Mexico as "Jamaica", "flor de Jamaica", has widely used in Mexican Traditional Medicine as antihypertensive and diuretic, although the latter activity has been reported the present work show evidence about the diuretic, natriuretic and potassium-sparing effects. To evaluate the diuretic activity of Hibiscus sabdariffa aqueous extract on in vivo and in situ models. The Hibiscus sabdariffa aqueous extract was administrated in increasing doses and evaluated the diuresis produced and disposal of electrolytes. Moreover, in isolated kidney was determined the renal filtration rate with plant extract, furosemide and amiloride. The yield of Hibiscus sabdariffa aqueous extraction was 28.3% and the chemical standardization from 1 g of extract was: 56.5 mg delphinidin-3-O-sambubioside, 20.8 mg/g cyanidin-3-O-sambubioside, 3.2 mg/g quercetin, 2.1 mg/g rutin and 2.7 mg/g chlorogenic acid. The diuretic and natriuretic effect of Hibiscus sabdariffa aqueous extract showed a dose-dependent behavior. The pharmacological constants of natriuretic effect was ED50=86 mg/kg and Emax=0.9 mEq/100 g/5 h. In the model of kidney in situ was observed that renal filtration increased 48% with the aqueous extract of Hibiscus sabdariffa and an additive effect when was perfuse with furosemide. The compound presents in Hibiscus sabdariffa as quercetin had effect on the vascular endothelium causing oxide nitric release, increasing renal vasorelaxation by increasing kidney filtration. Therefore, the diuretic effect of Hibiscus sabdariffa may be mediated by nitric oxide release. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  13. Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa

    OpenAIRE

    Ukoha, U. U.; Mbagwu, S. I.; Ndukwe, G. U.; Obiagboso, C.

    2015-01-01

    Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20) Wistar rats were randomly divided into four (4) groups of five rats each. The extract was administered orally in dos...

  14. BEES AS BIOINDICATORS TO GUARANTEE HEALTHY PRODUCTS FOR THE CONSUMER

    Directory of Open Access Journals (Sweden)

    B. Brusa

    2011-04-01

    Full Text Available Many investigators have employed honeybees or honeybee products as tools for assessing environmental pollution in industrial areas. The pollution in northwest Italy by insecticides used in crop protection, heavy metals and radioactivity has been investigated utilizing, as a bioindicator: honeybees, bee honey, wax, pollen produced in this area. Honeybees and honeybee products samples collected from 6 apiaries located in this area were analyzed for neonicotinoids residues with LC/MS method, pesticides organochlorines and organophosphates by GCECD and GC-NPD methods, PCB using GC-MS, radioactivity on 137Cs by g spectrometer and heavy metals with atomic spectroscopy. The results show: 19 honeybee samples were positive on neonicotinoids (clothianidin residues (total of 78 samples, no one sample was positive on pesticides organochlorines and organophosphates residues (total of 32 honeybee samples, the radioactivity levels were always below the instrumental limit determination, at last the heavy metal content (Pb, Cd, Cr on 21 honey samples was favorable. This study indicates that in agricultural areas with developed apiculture, useful information about the occurrence and the distribution of pesticide residues due to crop protection treatments can be derived from the analysis of randomly collected honeybee products samples, used as bioindicators.

  15. Potency of Mangrove Apple (Sonneratia alba as Mercury Bioindicator

    Directory of Open Access Journals (Sweden)

    Muhammad Reza Cordova

    2017-12-01

    Full Text Available The anthropogenic provide a negative impact on the surrounding environment. Mangrove species, such as Sonneratia alba would get the impact of anthropogenic activities, to accumulate the pollution of heavy metals. The aim of this study were to evaluate mercury accumulation in Mangrove Apple (S. alba and to analyze mangrove apple potency as mercury bioindicator. Samples were taken in April 2016 at Pari Island, Seribu Islands by purposive sampling. The results showed that the highest concentration of Hg in the Northern of Pari Island was found in the leaves and the lowest was in the fruit. The highest concentration of Hg in the Eastern of Pari Island was found in the leaves and lowest was in the fruit. The concentrations of Hg in the Eastern area higher the Northern area (significantly different. The accumulation of Hg mainly collected on the leaves with TF> 1, but the ability of S. alba trees absorb Hg in the environment showed a small value, namely BCF <1. The ability of S. alba in sediments, contaminated with mercury showed a high value of the leaves in the East Pari Island, but the fruit of S. alba both in the North and East of the Pari Island showed a small value.  Mangrove Apple leaves has a potency as mercury bioindicator organ.

  16. Lichen communities as bioindicators of the Aburra Valley air quality

    International Nuclear Information System (INIS)

    Jaramillo C, Margarita M; Botero B, Liliana R

    2010-01-01

    Lichens have been used as bioindicators of air quality. They are sensitive to changes in air composition at any given site affecting their abundance, biomass and vitality.This study evaluated lichen affectation as a consequence of air pollution. Two study areas within the Aburra Valley and the RedAire network were selected: Miguel Aguinaga (high pollution station) and the Medellin University (low pollution station). Four phorophytic tree species were chosen at each site Terminalia catappa Linneaus, Eritrina fusca Loureiro, Mangifera indica Linneaus y Fraxinus chinensis Roxb. The lichen cover by species, the vitality and the fructification were used to analyze the effect of air pollution. Finally, the lichen cover information was used to calculate the index of atmospheric purity (IAP) and the Q factor determination. Total qualitative and quantitative information obtained made evident that Canoparmelia sp. and Parmotrema austrosinensis (Zahlbr) Hale. lichen species are the most sensitive, and therefore appropriate to assess air quality. Additionally, the statistical analysis carried out using the relative abundances by phorophytic trees, showed that Fraxinus chinensis Roxb is the most appropriate tree species for bioindicator lichens studies into the Aburra valley.

  17. Bioindicator plants for ambient ozone in Central and Eastern Europe

    Energy Technology Data Exchange (ETDEWEB)

    Manning, W.J.; Godzik, B

    2004-07-01

    Sixteen species of native detector plants for ambient ozone have been identified for use in Central and Eastern Europe. They include the forbs Alchemilla sp., Astrantia major, Centuarea nigra, Centauria scabiosa, Impatiens parviflora, Lapsana communis, Rumex acetosa and Senecio subalpinus; the shrubs Corylus avellana, Cornus sanguinea and Sambucus racemosa; the trees Alnus incana, Pinus cembra and Sorbus aucuparia; and the vines Humulus lupulus and Parthenocissus quinquefolia. Sensitivity to ozone and symptoms have been verified under controlled exposure conditions. Under these conditions, symptom incidence, intensity and appearance often changed with time after removal from exposure chambers. Ozone sensitivity for four species: Astrantia major, Centuarea nigra, C. scabiosa and Humulus lupulus are reported here for the first time. The other 12 species have also been confirmed by others in Western Europe. It is recommended that these detector bioindicator species be used in conjunction with ozone monitors and passive samplers so that injury symptoms incidence can be used to give biological significance to monitored ambient ozone data. - Sixteen species of verified bioindicator plants for ambient ozone are available for use in Central and Eastern Europe.

  18. Effect of extract of Hibiscus on the ultrastructure of the testis in adult mice.

    Science.gov (United States)

    Mahmoud, Yomna Ibrahim

    2012-07-01

    Hibiscus sabdariffa extract is a popular beverage in many tropical and sub-tropical countries. Although, Hibiscus tea is known for its medicinal effects for thousands of years, scientific evidence of its systemic safety is very limited. The current study aimed to assess the potential adverse effects of H. sabdariffa extract on sperm morphology and testicular ultrastructure of albino mice. Thirty adult male albino mice were divided into three equal groups and were given: (a) distilled water, (b) cold Hibiscus aqueous extract, and (c) boiled Hibiscus aqueous extract. Hibiscus extract was administered orally daily for 4 weeks in a dose of 200 mg/kg body weight/mouse. Twenty-four hours after the last treatment, mice were decapitated and the testes and epididymides were excised and processed for transmission electron microscopy to assess ultrastructural and sperm abnormalities. The results clearly demonstrate that aqueous extracts from dried calyx of H. sabdariffa, either cold or boiled, alter normal sperm morphology and testicular ultrastructure and adversely influence the male reproductive fertility in albino mice. The current data suggest that Hibiscus extract should be consumed with caution, and reasonable estimates of the human risk associated with its consumption should be provided. Copyright © 2011 Elsevier GmbH. All rights reserved.

  19. Decrease of Plasma Glucose by Hibiscus taiwanensis in Type-1-Like Diabetic Rats

    Science.gov (United States)

    Wang, Lin-Yu; Chung, Hsien-Hui

    2013-01-01

    Hibiscus taiwanensis (Malvaceae) is widely used as an alternative herb to treat disorders in Taiwan. In the present study, it is used to screen the effect on diabetic hyperglycemia in streptozotocin-induced diabetic rats (STZ-diabetic rats). The extract of Hibiscus taiwanensis showed a significant plasma glucose-lowering action in STZ-diabetic rats. Stems of Hibiscus taiwanensis are more effective than other parts to decrease the plasma glucose in a dose-dependent manner. Oral administration of Hibiscus taiwanensis three times daily for 3 days into STZ-diabetic rats increased the sensitivity to exogenous insulin showing an increase in insulin sensitivity. Moreover, similar repeated administration of Hibiscus taiwanensis for 3 days in STZ-diabetic rats produced a marked reduction of phosphoenolpyruvate carboxykinase (PEPCK) expression in liver and an increased expression of glucose transporter subtype 4 (GLUT 4) in skeletal muscle. Taken together, our results suggest that Hibiscus taiwanensis has the ability to lower plasma glucose through an increase in glucose utilization via elevation of skeletal GLUT 4 and decrease of hepatic PEPCK in STZ-diabetic rats. PMID:23690841

  20. A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L.

    Science.gov (United States)

    Riaz, Ghazala; Chopra, Rajni

    2018-03-26

    Hibiscus sabdariffa L. (roselle) belonging to the Malvaceae family is widely grown in many countries. This plant is often used in the traditional medicine being rich in phytochemicals like polyphenols especially anthocyanins, polysaccharides and organic acids thus having enormous prospective in modern therapeutic uses. The study aimed to review and document all the available evidence and information about the calyces of Hibiscus sabdariffa (roselle) with the special focus on their nutritional composition, bioactive constituents and therapeutic uses. The electronic database was searched up to 2017, using keywords Hibiscus sabdariffa, chemical constituents of roselle, therapeutic uses of roselle. Journals, books and conference proceedings were also searched. The review provides valuable information about the nutraceutical component of Hibiscus sabdariffa L. and their utilization for curing various degenerative diseases like hypertension, hyperlipidemia, cancer and other inflammatory diseases of liver and kidney. Their toxicological effects have also been discussed from a safety point of view. Most studies supported and provided the scientific basis for the statement that Hibiscus sabdariffa and their active constituents play an important role in the prevention of chronic and degenerative diseases that are associated with oxidative stress. Our study suggests, that good research is needed, to establish a potential strategy that can balance the pharmacological and toxic effects of roselle and standardized fingerprint of Hibiscus sabdariffa is required internationally for quality control. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  1. The effectiveness of Hibiscus sabdariffa in the treatment of hypertension: a systematic review.

    Science.gov (United States)

    Wahabi, H A; Alansary, L A; Al-Sabban, A H; Glasziuo, P

    2010-02-01

    Hypertension is a common global health problem with significant mortality and morbidity. Hibiscus sabdariffa is a plant known in many countries and is consumed as hot and cold drinks In addition to its use in folk medicine; it has been suggested as treatment for many conditions including hypertension. The objectives of this review were to examine the evidence of effectiveness and safety of hibiscus in the treatment of hypertension. We searched several medical databases (MEDLINE, EMBASE, CINAHL, Cochrane Central Register of Controlled Trials, and the specialized register of the Cochrane Hypertension Group and the general engine Google) to January 2009. We included randomized controlled trials that had examined Hibiscus's effectiveness and safety in the treatment of primary hypertension in adults. Two authors independently selected the trials for the review, extracted the data, and critically appraised the included studies. Four trials, with a total of 390 patients, met our inclusion criteria. Two studies compared Hibiscus sabdariffa to black tea; one study compared it to captopril and one to lisinopril. The studies found that Hibiscus had greater blood pressure reduction than tea but less than the ACE-inhibitors. However, all studies, except one, were short term and of poor quality with a Jadad scoring of Hibiscus sabdariffa for the treatment of primary hypertension in adults. Copyright 2009 Elsevier GmbH. All rights reserved.

  2. Spatial and temporal transcriptome changes occurring during flower opening and senescence of the ephemeral hibiscus flower, Hibiscus rosa-sinensis.

    Science.gov (United States)

    Trivellini, Alice; Cocetta, Giacomo; Hunter, Donald A; Vernieri, Paolo; Ferrante, Antonio

    2016-10-01

    Flowers are complex systems whose vegetative and sexual structures initiate and die in a synchronous manner. The rapidity of this process varies widely in flowers, with some lasting for months while others such as Hibiscus rosa-sinensis survive for only a day. The genetic regulation underlying these differences is unclear. To identify key genes and pathways that coordinate floral organ senescence of ephemeral flowers, we identified transcripts in H. rosa-sinensis floral organs by 454 sequencing. During development, 2053 transcripts increased and 2135 decreased significantly in abundance. The senescence of the flower was associated with increased abundance of many hydrolytic genes, including aspartic and cysteine proteases, vacuolar processing enzymes, and nucleases. Pathway analysis suggested that transcripts altering significantly in abundance were enriched in functions related to cell wall-, aquaporin-, light/circadian clock-, autophagy-, and calcium-related genes. Finding enrichment in light/circadian clock-related genes fits well with the observation that hibiscus floral development is highly synchronized with light and the hypothesis that ageing/senescence of the flower is orchestrated by a molecular clock. Further study of these genes will provide novel insight into how the molecular clock is able to regulate the timing of programmed cell death in tissues. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  3. Aqueous Extracts of Hibiscus sabdariffa Calyces to Control Aichi Virus.

    Science.gov (United States)

    D'Souza, Doris H; Dice, Lezlee; Davidson, P Michael

    2016-06-01

    Aqueous Hibiscus sabdariffa extracts possess antimicrobial properties with limited information available on their antiviral effects. Aichi virus (AiV) is an emerging foodborne pathogen that causes gastroenteritis. Vaccines are currently unavailable to prevent their disease transmission. The objective of this study was to determine the antiviral effects of aqueous H. sabdariffa extracts against AiV. AiV at ~5 log PFU/ml was incubated with undiluted (200 mg/ml), 1:1 (100 mg/ml) or 1:5 (40 mg/ml) diluted aqueous hibiscus extract (pH 3.6), phosphate-buffered saline (pH 7.2 as control), or malic acid (pH 3.0, acid control) at 37 °C over 24 h. Treatments were stopped by serially diluting in cell-culture media containing fetal bovine serum and titers were determined using plaque assays on confluent Vero cells. Each treatment was replicated thrice and assayed in duplicate. AiV did not show any significant reduction with 1:1 (100 mg/ml) or 1:5 (40 mg/ml) diluted aqueous hibiscus extracts or malic acid after 0.5, 1, or 2 h at 37 °C. However, AiV titers were reduced to non-detectable levels after 24 h with all the three tested concentrations, while malic acid showed only 0.93 log PFU/ml reduction after 24 h. AiV was reduced by 0.5 and 0.9 log PFU/ml with undiluted extracts (200 mg/ml) after 2 and 6 h, respectively. AiV treated with 1:1 (100 mg/ml) and 1:5 (40 mg/ml) diluted extracts showed a minimal ~0.3 log PFU/ml reduction after 6 h. These extracts show promise to reduce AiV titers mainly through alteration of virus structure, though higher concentrations may have improved effects.

  4. Vegetarian bio-indicators for environmental monitoring; Bioindicatori vegetali per il monitoraggio ambientale

    Energy Technology Data Exchange (ETDEWEB)

    Leva, F.; Dapiaggi, M.; Cenci, R.M. [Centro Consune di Ricerca di Ispra (Italy)

    2001-07-01

    The most common bio-indicators used for the evaluation of various contaminants, are reviewed. The aim has been to make clearly understood the difference between visual analysis (bio-indication) and bio-accumulation, by foccussing on practical aspects, the advantages and disadvantages in the use of bio-indicators. Some examples included in the review are the use of tobacco leaves for the evaluation of the presence of ozone, of lichens for the presence of SO{sub 2}, and of mosses for the determination of heavy metal concentration.

  5. Protocols for Callus and Somatic Embryo Initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of Explant Type, Sugar, and Plant Growth Regulators

    Science.gov (United States)

    A significant work on callus induction and somatic embryogenesis was realized for Hibiscus sabdariffa. Two genotypes (Hibiscus sabdariffa and Hibiscus sabdariffa var. altissima) two sugars (sucrose and glucose) and three concentrations (1 %, 2%, 3%) of each sugar, 3 explant types (root, hypocotyl, c...

  6. Vitiquinolone--a quinolone alkaloid from Hibiscus vitifolius Linn.

    Science.gov (United States)

    Ramasamy, D; Saraswathy, A

    2014-02-15

    Phytochemical investigations of the powdered root of Hibiscus vitifolius Linn. (Malvaceae) was extracted successively with n-hexane and chloroform. Analysis of the n-hexane extract by GC-MS led to the identification of twenty-six components by comparison of their mass spectra with GC-MS library data. A novel quinolone alkaloid, vitiquinolone (5) together with eight known compounds viz. β-Amyrin acetate (1), n-octacosanol (2), β-Amyrin (3), stigmasterol (4), xanthyletin (6), alloxanthoxyletin (7), xanthoxyletin (8) and betulinic acid (9) were isolated from chloroform extract by column chromatography over silica gel. The structure of vitiquinolone was established on the basis of spectroscopic methods including UV, IR, 1D, 2D NMR and ESI-MS. The known compounds were identified on the basis of their physical and spectroscopic data as reported in the literature. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Hibiscus sabdariffa extract lowers blood pressure and improves endothelial function.

    Science.gov (United States)

    Joven, Jorge; March, Isabel; Espinel, Eugenia; Fernández-Arroyo, Salvador; Rodríguez-Gallego, Esther; Aragonès, Gerard; Beltrán-Debón, Raúl; Alonso-Villaverde, Carlos; Rios, Lidia; Martin-Paredero, Vicente; Menendez, Javier A; Micol, Vicente; Segura-Carretero, Antonio; Camps, Jordi

    2014-06-01

    Polyphenols from Hibiscus sabdariffa calices were administered to patients with metabolic syndrome (125 mg/kg/day for 4 wk, n = 31) and spontaneously hypertensive rats (125 or 60 mg/kg in a single dose or daily for 1 wk, n = 8 for each experimental group). The H. sabdariffa extract improved metabolism, displayed potent anti-inflammatory and antioxidant activities, and significantly reduced blood pressure in both humans and rats. Diuresis and inhibition of the angiotensin I-converting enzyme were found to be less important mechanisms than those related to the antioxidant, anti-inflammatory, and endothelium-dependent effects to explain the beneficial actions. Notably, polyphenols induced a favorable endothelial response that should be considered in the management of metabolic cardiovascular risks. © 2014 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Factors influencing extract of Hibiscus sabdariffa staining of rat testes.

    Science.gov (United States)

    Bassey, R B; Bakare, A A; Peter, A I; Oremosu, A A; Osinubi, A A

    2012-08-01

    Some plant extracts can be used in biology and medicine to reveal or identify cellular components and tissues. We investigated the effects of time and concentration on staining of histological sections of rat testes by an acidified extract of Hibiscus sabdariffa. An ethanolic extract of H. sabdariffa was diluted using 1% acetic acid in 70% ethanol to stain histological sections of testes at concentrations of 0.2, 0.1 and 0.05 g/ml for 5, 10, 15, 30, 45 and 60 min. The sections of testes were stained deep red. The staining efficiency of H. sabdariffa was greater at a high concentration and required less time to achieve optimal staining. H. sabdariffa is a strongly basic dye that can be used for various diagnostic purposes. Staining time and concentration must be considered to achieve optimal results.

  9. Natural cross-pollination in roselle, Hibiscus sabdariffa L. (Malvaceae

    Directory of Open Access Journals (Sweden)

    Vaidya K.R.

    2000-01-01

    Full Text Available Two local varieties of roselle (Hibiscus sabdariffa L., Jamaican Green and Jamaican Red, were grown to determine the amount of natural cross-pollination. Two planting arrangements (alternating rows; alternating individuals in a row and two planting dates, a month apart, were used for the outcrossing experiments. Stem pigmentation, red (R- vs. green (rr, was used as a genetic marker in the estimation of outcrossing. Homozygous dominant and recessive genotypes of Jamaican Red and Jamaican Green, respectively, were grown in both of the planting arrangements and dates. Seeds from open-pollinated capsules of randomly selected Jamaican Green plants were planted to score the frequency of cross-pollination. Estimates of natural cross-pollination ranged from 0.20 ± 0.09% to 0.68 ± 0.34%. Roselle outcrosses at a low rate in Jamaica.

  10. Mussels as bioindicators of diclofenac contamination in coastal environments.

    Science.gov (United States)

    Cunha, S C; Pena, A; Fernandes, J O

    2017-06-01

    Diclofenac a nonsteroidal anti-inflammatory drug (NSAID) has been confirmed as an emerging contaminant in the aquatic environment. Toxicology studies have revealed that harmful effects may emerge from diclofenac presence not only for human health, but also for marine organisms, which implies its monitoring. To overcome the demanding challenges of diclofenac quantification in biotic aquatic species, a novel method for the determination of diclofenac in mussels (Mytilus galloprovincialis and Mytilus edulis) and macroalgae (Laminaria digitata) using high performance liquid chromatography coupled to tandem mass spectrometry was developed and validated according to the EC Decision 2002/657/EC. Additionally, a study was done about diclofenac contamination in mussels collected from 8 sites along the 1115 miles of coastline in Portugal in 2015. The results suggested that levels in mussels are closely related to the environmental contamination. Therefore, mussels can be a potential bioindicator of diclofenac contamination in the coastal environment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Bioindicators in the tropical forest of Kaiga environment

    Energy Technology Data Exchange (ETDEWEB)

    Somashekarappa, H.M.; Narayana, Y.; Radhakrishna, A.P.; Karunakara, N.; Balakrishna, K.M.; Siddappa, K. [Mangalore Univ. (India). Dept. of Physics

    1996-07-01

    Investigations on the natural and artificial fallout radionuclides {sup 210}Po and {sup 137}Cs and the primordial radionuclide {sup 40}K in the prominent tree species of Western Ghat tropical forests near Kaiga have been carried out as a part of baseline background radiation studies in the environment of Kaiga where nuclear power reactors are being installed. The prominent tree species of the region Tectona grandis L.f. and Terminalia paniculata Roth., and the commonly available epiphytic plant species Pterobryopsis tumida (Hook.) Dix. and Cymbidium aliofolium (Lo) Swartz. were chosen and concentrations of {sup 40}K, {sup 210}Po and {sup 137}Cs were measured employing well-established nuclear techniques. The different parts of Cumbidium aloifolium (Lo) Swartz. such as leaves, stem, etc. were analysed to understand the absorption mechanism of fallout radionuclides. From a careful analysis of the results, the epiphytic plant species are identified as bioindicators to monitor fallout radionuclides. (Author).

  12. Multibiomarker assessment of three Brazilian estuaries using oysters as bioindicators

    International Nuclear Information System (INIS)

    Valdez Domingos, F.X.; Azevedo, M.; Silva, M.D.; Randi, M.A.F.; Freire, C.A.; Silva de Assis, H.C.; Oliveira Ribeiro, C.A.

    2007-01-01

    Oysters have been largely employed as bioindicators of environmental quality in biomonitoring studies. Crassostrea rhizophorae was selected to evaluate the health status of three estuarine areas impacted by anthropogenic activities along the Brazilian coast, in three estuarine complexes, ranging in latitude from 7 to 25 deg. S. In each estuary three sites were sampled in Winter and in Summer: a site considered as reference, and two sites next to contamination sources. Condition index was similar at all sites and estuaries, with the highest values found for Itamaraca oysters in Summer. Necrosis, hyperplasia, mucocyte hypertrophy and fusion of ordinary filaments were the main histopathological lesions observed. Muscle cholinesterase activity was overall similar, but with a strong seasonal effect. Inhibition or activation of branchial total ATPase and Na,K-ATPase activities at the contaminated sites was observed. The health status of these estuarine areas is quite similar, and the combined use of biomarkers is recommended

  13. Bioindicators in the tropical forest of Kaiga environment

    International Nuclear Information System (INIS)

    Somashekarappa, H.M.; Narayana, Y.; Radhakrishna, A.P.; Karunakara, N.; Balakrishna, K.M.; Siddappa, K.

    1996-01-01

    Investigations on the natural and artificial fallout radionuclides 210 Po and 137 Cs and the primordial radionuclide 40 K in the prominent tree species of Western Ghat tropical forests near Kaiga have been carried out as a part of baseline background radiation studies in the environment of Kaiga where nuclear power reactors are being installed. The prominent tree species of the region Tectona grandis L.f. and Terminalia paniculata Roth., and the commonly available epiphytic plant species Pterobryopsis tumida (Hook.) Dix. and Cymbidium aliofolium (Lo) Swartz. were chosen and concentrations of 40 K, 210 Po and 137 Cs were measured employing well-established nuclear techniques. The different parts of Cumbidium aloifolium (Lo) Swartz. such as leaves, stem, etc. were analysed to understand the absorption mechanism of fallout radionuclides. From a careful analysis of the results, the epiphytic plant species are identified as bioindicators to monitor fallout radionuclides. (Author)

  14. Assessment of oil content and fatty acid composition variability in two economically important Hibiscus species.

    Science.gov (United States)

    Wang, Ming Li; Morris, Brad; Tonnis, Brandon; Davis, Jerry; Pederson, Gary A

    2012-07-04

    The Hibiscus genus encompasses more than 300 species, but kenaf (Hibiscus cannabinus L.) and roselle (Hibiscus sabdariffa L.) are the two most economically important species within the genus. Seeds from these two Hibiscus species contain a relatively high amount of oil with two unusual fatty acids: dihydrosterculic and vernolic acids. The fatty acid composition in the oil can directly affect oil quality and its utilization. However, the variability in oil content and fatty acid composition for these two species is unclear. For these two species, 329 available accessions were acquired from the USDA germplasm collection. Their oil content and fatty acid composition were determined by nuclear magnetic resonance (NMR) and gas chromatography (GC), respectively. Using NMR and GC analyses, we found that Hibiscus seeds on average contained 18% oil and seed oil was composed of six major fatty acids (each >1%) and seven minor fatty acids (each Hibiscus cannabinus seeds contained significantly higher amounts of oil (18.14%), palmitic (20.75%), oleic (28.91%), vernolic acids (VA, 4.16%), and significantly lower amounts of stearic (3.96%), linoleic (39.49%), and dihydrosterculic acids (DHSA, 1.08%) than H. sabdariffa seeds (17.35%, 18.52%, 25.16%, 3.52%, 4.31%, 44.72%, and 1.57%, respectively). For edible oils, a higher oleic/linoleic (O/L) ratio and lower level of DHSA are preferred, and for industrial oils a high level of VA is preferred. Our results indicate that seeds from H. cannabinus may be of higher quality than H. sabdariffa seeds for these reasons. Significant variability in oil content and major fatty acids was also detected within both species. The variability in oil content and fatty acid composition revealed from this study will be useful for exploring seed utilization and developing new cultivars in these Hibiscus species.

  15. Use of octopus as a bioindicator species: Baseline studies

    Energy Technology Data Exchange (ETDEWEB)

    Holdway, D.A.; Butty, J.S.; Brennan, S.E.; Ahokas, J.T. [RMIT-Univ., Victoria (Australia). Key Centre for Applied and Nutritional Toxicology

    1995-12-31

    The Australian octopus Octopus pallidus, is abundant, territorial, has a large digestive gland. This study was undertaken to assess octopii as a potential bioindicator species by establishing the efficacy of capturing octopi using traplines, and determining the impact of various modifying factors on the activities of digestive gland mixed-function oxidase (MFO) enzymes including ethoxyresorufin O-deethylase (EROD), ethoxycoumarin O-deethylase (ECOD) and total P-450. Trap success rates in Port Phillip Bay were 15--28% for the ``potentially contaminated`` site and 85% for the reference site. Cytochrome P-450 showed significant seasonal differences, with no site or sex differences. Mean ({+-} SE) Autumn P-450 values of 74.8 ({+-}5.5) pmol/mg protein were higher than Winter values of 51.2 ({+-}7.6), which were higher than Spring values of 21.8 ({+-}4.0) pmol/mg protein. Summer P-450 values of 61.4 ({+-} 9.8) pmol/mg protein were only different from Spring values. Mean ({+-} SE) Spring ECOD activity of 3.3 ({+-} 0.7) pmol/min/mg protein was lower than Summer, Autumn and Winter ECOD values of 8.9 ({+-} 1.6) 6.5 ({+-} 1.2) and 8.6 ({+-} 2.3) pmol/min/mg protein respectively. Females had roughly half the ECOD activities of males (3.8 {+-} 0.8 compared to 7.4 {+-} 0.9 pmol/min/mg protein). All octopi digestive gland EROD activities were low (roughly 0.2 pmol/min/mg protein) with no sex, site nor seasonal differences. Potential for using octopus as a bioindicator appears promising but sensitivity to chemical exposure has yet to be determined.

  16. USEPA BIOMONITORING AND BIOINDICATORS CONCEPTS NEEDED TO EVALUATE THE BIOLOGICAL INTEGRITY OF AQUATIC SYSTEMS

    Science.gov (United States)

    This chapter presents the current uses, concepts and anticipated future directions of biomonitoring and bioindicators in the regulatory and research programs of the United States Environmental Protection Agency (USEPA). The chapter provides a historical look on how biomonitoring ...

  17. The amphipod Orchomenella pinguis--a potential bioindicator for contamination in the Arctic.

    Science.gov (United States)

    Bach, Lis; Forbes, Valery E; Dahllöf, Ingela

    2009-11-01

    Indigenous organisms can be used as bioindicators for effects of contaminants, but no such bioindicator has been established for Arctic areas. Orchomenella pinguis is a benthic amphipod, ubiquitous in the Arctic and can be found in high numbers. We collected O. pinguis at sites with different contamination levels. Population characteristics (body length distribution, average dry weight and amphipod organic content) were related to sediment contaminant concentrations, in order to identify suitable endpoints for using this species as a bioindicator. We show that O. pinguis was prevalent in both clean and contaminated areas, easy to sample and that its population characteristics could be linked to both contamination and sediment organic content. We suggest that O. pinguis is a suitable bioindicator for the Arctic, but that endpoints such as reproductive effects and phenotypic and genotypic responses are needed together with population characteristics to assess impacts of contamination.

  18. US EPA BIOMONITORING AND BIOINDICATOR CONCEPTS NEEDED TO EVALUATE THE BIOLOGICAL INTEGRITY OF AQUATIC SYSTEMS

    Science.gov (United States)

    This chapter presents the current uses, concepts and anticipated future directions of biomonitoring and bioindicators in the regulatory and research programs of the United States Environmental Protection Agency (USEPA). The chapter provides a historical look on how biomonitoring ...

  19. Notice to nurserymen of the nameing and release for propagation of lufin white an american hardy hibiscus cultivar

    Science.gov (United States)

    Hibiscus laevis ‘LUFKIN WHITE’, a new hardy native ornamental hibiscus cultivar is recommended for trial by nurserymen and horticulturists as a summer flowering perennial landscape plant nationwide but is particularly adapted to conditions in the South. ‘LUFKIN WHITE’ was selected for its exception...

  20. Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species.

    Science.gov (United States)

    Kapepula, Paulin Mutwale; Kabamba Ngombe, Nadege; Tshisekedi Tshibangu, Pascal; Tsumbu, César; Franck, Thierry; Mouithys-Mickalad, Ange; Mumba, Dieudonné; Tshala-Katumbay, Désiré; Serteyn, Didier; Tits, Monique; Angenot, Luc; Kalenda, Pascal Dibungi T; Frédérich, Michel

    2017-12-01

    Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC 50 values ranging from 0.5 to 3 μg mL -1 using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.

  1. Xyloglucan, hibiscus and propolis for the prevention of urinary tract infections: results of in vitro studies.

    Science.gov (United States)

    Fraile, Benito; Alcover, Javier; Royuela, Mar; Rodríguez, David; Chaves, Concepción; Palacios, Ricardo; Piqué, Núria

    2017-06-01

    To assess the properties of a medical device containing xyloglucan, propolis and hibiscus to create a bioprotective barrier to avoid the contact of uropathogenic Escherichia coli strains on cell walls in models of intestinal (CacoGoblet) and uroepithelial (RWPE-1) cells (derived from normal human prostate epithelium). Two uropathogenic E. coli strains (expressing type 1 fimbriae and P fimbriae) were used to assess, by electronic microscopy and ELISA, the barrier properties of the medical device. The antimicrobial activity was assessed in broth dilution assays. The three components (xyloglucan, propolis and hibiscus) did not alter E. coli cell integrity in intestinal and uroepithelial cell models and were devoid of antibacterial activity. The three components avoided bacterial contact in both cell monolayers. The nonpharmacological barrier properties of xyloglucan, propolis and hibiscus confirm the role of the medical device for the management of urinary tract infections.

  2. [Pharmacological study on hemostasis, analgesic and anti inflammation effects of the alcohol extract of Hibiscus tiliaceus].

    Science.gov (United States)

    Qiu, Fen; Tian, Hui; Zhang, Zhi; Yuan, Xian-Ling; Tan, Yuan-Feng; Ning, Xiao-Qing

    2013-10-01

    To study the effects of hemostasis, analgesic and anti inflammation of the alcohol extract of Hibiscus tiliaceus and offer pharmacological and experimental basis for its safe and effective use in clinic. The effects of hemostasist were observed with tail breaking method, capillary tube method and slide method; Hot board and body distortion induced by acetic acid methods were applied in mice analgesia experiment, the mice model of acute auricle swelling induced by dmi ethylbenzene and capillary permeability induced by acetic acid were applied to observe the anti inflammatory effects. The alcohol extract of Hibiscus tiliaceus could significantly reduce the bleeding time and the clotting time, delay the plant reaction time and reduce the writhing times of the mice, and it also had effect on inhibiting swelling of mice ear and the permeability of the capillary. These results suggest that the alcohol extract of Hibiscus tiliaceus has the effects of hemostasis, analgesic and anti inflammation.

  3. Breeding of Hibiscus rosa-sinensis for garden use in Denmark

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk

    2013-01-01

    Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa-sinensis...... for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa-sinensis...... suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities...

  4. Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa

    Directory of Open Access Journals (Sweden)

    U. U. Ukoha

    2015-01-01

    Full Text Available Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20 Wistar rats were randomly divided into four (4 groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.

  5. Remote Sensing of Bioindicators for Forest Health Assessment

    Science.gov (United States)

    Kefauver, Shawn Carlisle

    The impacts of tropospheric ozone on forest health in Mediterranean type climates in California, USA and Catalonia, Spain were investigated using a combination of remote sensing, Geographic Information System (GIS), and field studies focused on sensitive bioindicator conifer species and ambient ozone monitoring. For the field validation of impacts of tropospheric ozone on conifer health, the Ozone Injury Index (OII) was applied to the bioindicator species Pinus ponderosa, Pinus jeffreyi, and Pinus uncinata. Combining these three tools, it was possible to build meaningful ecological models covering large areas to enhance our understanding of the biotic and abiotic interactions which affect forest health. Regression models predicting ozone injury improved considerably when incorporating ozone exposure with GIS related to plant water status, including water availability and water usage, as a proxies for estimating the stomatal conductance and ozone uptake R2=0.35, p = 0.016 in Catalonia, R2=0.36, p < 0.001 in Yosemite and R2=0.33, p = 0.007 in Sequoia/Kings Canyon National Parks in California). Individual OII components in Catalonia were modeled with improved success compared to the original full OII, in particular visible chlorotic mottling (R2=0.60, p < 0.001). The visual chlorotic mottling component of the OII was the most strongly correlated to remote sensing indices, in particular the photochemical reflectance index (PRI; R2=0.28, p=0.0044 for OIIVI-amount and R 2=0.33 and p=0.0016 for OIIVI -severity). Regression models assessing ozone injury to conifers using imaging spectroscopy techniques also improved when incorporating the GIS proxies of stomatal conductance (R 2=0.59, p<0.0001 for OII in California and R2=0.68, p<0.0001 for OIIVI in Catalonia). Finally, taking advantage of a time series of ambient ozone monitoring in Catalonia, it was found that all models improved when incorporating the cumulative exposure to ozone over a period of three years (R2=0.56, p

  6. Hibiscus sabdariffa L. tea (tisane) lowers blood pressure in prehypertensive and mildly hypertensive adults.

    Science.gov (United States)

    McKay, Diane L; Chen, C-Y Oliver; Saltzman, Edward; Blumberg, Jeffrey B

    2010-02-01

    In vitro studies show Hibiscus sabdariffa L., an ingredient found in many herbal tea blends and other beverages, has antioxidant properties, and, in animal models, extracts of its calyces have demonstrated hypocholesterolemic and antihypertensive properties. Our objective in this study was to examine the antihypertensive effects of H. sabdariffa tisane (hibiscus tea) consumption in humans. A randomized, double-blind, placebo-controlled clinical trial was conducted in 65 pre- and mildly hypertensive adults, age 30-70 y, not taking blood pressure (BP)-lowering medications, with either 3 240-mL servings/d of brewed hibiscus tea or placebo beverage for 6 wk. A standardized method was used to measure BP at baseline and weekly intervals. At 6 wk, hibiscus tea lowered systolic BP (SBP) compared with placebo (-7.2 +/- 11.4 vs. -1.3 +/- 10.0 mm Hg; P = 0.030). Diastolic BP was also lower, although this change did not differ from placebo (-3.1 +/- 7.0 vs. -0.5 +/- 7.5 mm Hg; P = 0.160). The change in mean arterial pressure was of borderline significance compared with placebo (-4.5 +/- 7.7 vs. -0.8 +/- 7.4 mm Hg; P = 0.054). Participants with higher SBP at baseline showed a greater response to hibiscus treatment (r = -0.421 for SBP change; P = 0.010). No effects were observed with regard to age, gender, or dietary supplement use. These results suggest daily consumption of hibiscus tea, in an amount readily incorporated into the diet, lowers BP in pre- and mildly hypertensive adults and may prove an effective component of the dietary changes recommended for people with these conditions.

  7. Z-scan and optical limiting properties of Hibiscus Sabdariffa dye

    Science.gov (United States)

    Diallo, A.; Zongo, S.; Mthunzi, P.; Rehman, S.; Alqaradawi, S. Y.; Soboyejo, W.; Maaza, M.

    2014-12-01

    The intensity-dependent refractive index n 2 and the nonlinear susceptibility χ (3) of Hibiscus Sabdariffa dye solutions in the nanosecond regime at 532 nm are reported. More presicely, the variation of n 2, β, and real and imaginary parts of χ (3) versus the natural dye extract concentration has been carried out by z-scan and optical limiting techniques. The third-order nonlinearity of the Hibiscus Sabdariffa dye solutions was found to be dominated by nonlinear refraction, which leads to strong optical limiting of laser.

  8. Hot and cold water infusion aroma profiles of Hibiscus sabdariffa: fresh compared with dried.

    Science.gov (United States)

    Ramírez-Rodrigues, M M; Balaban, M O; Marshall, M R; Rouseff, R L

    2011-03-01

    Calyxes from the Roselle plant (Hibiscus sabdariffa L.) were used to prepare cold (22 °C for 4 h) and hot (98 °C for 16 min) infusions/teas from both fresh and dried forms. Aroma volatiles were extracted using static headspace SPME and analyzed using GC-MS and GC-O with 2 different columns (DB-5 and DB-Wax). Totals of 28, 25, 17, and 16 volatiles were identified using GC-MS in the dried hot extract (DHE), dried cold extract (DCE), fresh hot extract (FHE), and fresh cold extract (FCE) samples, respectively. In terms of total GC-MS peak areas DHE ≫ DCE > FHE ≫ FCE. Nonanal, decanal, octanal, and 1-octen-3-ol were among the major volatiles in all 4 beverage types. Thirteen volatiles were common to all 4 teas. Furfural and 5-methyl furfural were detected only in dried hibiscus beverages whereas linalool and 2-ethyl-1-hexanol were detected only in beverages from fresh hibiscus. In terms of aroma active volatiles, 17, 16, 13, and 10 aroma active volatiles were detected for DHE, DCE, FHE, and FCE samples, respectively. The most intense aroma volatiles were 1-octen-3-one and nonanal with a group of 4 aldehydes and 3 ketones common to all samples. Dried samples contained dramatically higher levels of lipid oxidation products such as hexanal, nonanal, and decanal. In fresh hibiscus extracts, linalool (floral, citrus) and octanal (lemon, citrus) were among the highest intensity aroma compounds but linalool was not detected in any of the dried hibiscus extracts. Hibiscus teas/infusions are one of the highest volume specialty botanical products in international commerce. The beverage is consumed for both sensory pleasure and health attributes and is prepared a number of ways throughout the world. Although color and taste attributes have been examined, little information is known about its aroma volatiles and no other study has compared extractions from both fresh and dried as well as extraction temperature differences. This is also, apparently, the first study to identify

  9. Forest avifauna as a bioindicator of heavy metal pollution

    Science.gov (United States)

    Costa, Rute Alexandra Pais

    Air pollution has become a widespread problem in the last century, becoming necessary the monitorization of several habitats. Air pollution was found to have direct and indirect effects on forest passerines (Eeva et al. 1997), but there is very little information on the effects of emissions from the paper and pulp industry. The present work includes a series of studies which main goals were to use non-invasive procedures in the evaluation of forest passerines as bioindicators of heavy metal pollution and to assess the possible influence of pollution in birds’ breeding biology and health status in industrial and rural sites in maritime pine forests on the west coast of Portugal. We found higher arsenic levels in the rural area and higher mercury levels in the industrial area but we also found several differences with significantly lower levels of contamination in 2010 and 2011. We found that Great tits bred earlier, laid more eggs and produced more fledglings in the industrial area, where we also found higher caterpillar biomass, which are an important food source for tits. Health indices presented similar results in both areas and comparing to other studies in Europe the values are consistent with good health conditions. Our results suggest that there are no direct toxic effects of emissions from the paper industry on the study species. However, invertebrate food availability seems to be related to pollution levels, which indirectly affect the breeding performance of the Great tit.

  10. Leeches as Sensor-bioindicators of River Contamination by PCBs

    Directory of Open Access Journals (Sweden)

    Gorzyslaw Poleszczuk

    2009-03-01

    Full Text Available The aim of the study was to evaluate the use of leeches of the genus Erpobdella as a means of assessing polychlorinated biphenyl contamination of watercourses. The River Skalice, heavily contaminated with PCBs, was selected as a model. The source of contamination was a road gravel processing factory in Rožmitál pod Třemšínem from which an estimated 1 metric ton of PCBs leaked in 1986. Levels of PCB were measured in leeches collected between 1992 to 2003 from 11 sites covering about 50 km of the river (the first sampling site upstream to the source of contamination and 10 sites downstream. The PCB indicator congeners IUPA no. 28, 52, 101, 118, 138, 153, and 180 were measured. Levels were highest at the four sampling sites nearest the source of pollution. The highest values of PCB congeners were found in 1992. PCB content decreased from 1992 to 2003 and with distance from the source. The study indicated that leeches of the genus Erpobdella are a suitable bioindicator of contamination in the surface layer of river sediments.

  11. Bioindicator plants for ambient ozone in Central and Eastern Europe.

    Science.gov (United States)

    Manning, W J; Godzik, B

    2004-07-01

    Sixteen species of native detector plants for ambient ozone have been identified for use in Central and Eastern Europe. They include the forbs Alchemilla sp., Astrantia major, Centuarea nigra, Centauria scabiosa, Impatiens parviflora, Lapsana communis, Rumex acetosa and Senecio subalpinus; the shrubs Corylus avellana, Cornus sanguinea and Sambucus racemosa; the trees Alnus incana, Pinus cembra and Sorbus aucuparia; and the vines Humulus lupulus and Parthenocissus quinquefolia. Sensitivity to ozone and symptoms have been verified under controlled exposure conditions. Under these conditions, symptom incidence, intensity and appearance often changed with time after removal from exposure chambers. Ozone sensitivity for four species: Astrantia major, Centuarea nigra, C. scabiosa and Humulus lupulus are reported here for the first time. The other 12 species have also been confirmed by others in Western Europe. It is recommended that these detector bioindicator species be used in conjunction with ozone monitors and passive samplers so that injury symptoms incidence can be used to give biological significance to monitored ambient ozone data.

  12. Active caspase-3 expression levels as bioindicator of individual radiosensitivity

    Directory of Open Access Journals (Sweden)

    NEYLIANE F.G. DOS SANTOS

    Full Text Available ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source with 1, 2 and 4 Gy (control: non-irradiated samples, and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours. Short-term incubation of 24 h was the most adequate condition to evidence correlations between dose radiation and active caspase-3 expression. For each radiation dose, it was observed a significant inter-individual variation in active caspase-3 expression intensity, suggesting that this parameter may be suitable for evidence individual radiosensitivity. The methodology presented and discussed in this work may help to predict healthy tissues response to radiation exposure toward the better patient outcome.

  13. Mussel Shell Evaluation as Bioindicator For Heavy Metals

    Science.gov (United States)

    Andrello, Avacir Casanova; Lopes, Fábio; Galvão, Tiago Dutra

    2010-05-01

    Recently, in Brazil, it has appeared a new and unusual "plague" in lazer and commercial fishing. It is caused by the parasitic larval phase of certain native bivalve mollusks of fresh water known as "Naiades" and its involves the presence of big bivalve of fresh water, mainly Anodontites trapesialis, in the tanks and dams of the fish creation. These bivalve mollusks belong to the Unionoida Order, Mycetopodidae Family. The objective of the present work was to analyze the shells of these mollusks to verify the possibility of use as bioindicators for heavy metals in freshwater. The mollusks shells were collected in a commercial fishing at Londrina-PR. A qualitative analysis was made to determine the chemical composition of the shells and verify a possible correlation with existent heavy metals in the aquatic environment. In the inner part of the shells were identified the elements Ca, P, Fe, Mn and Sr and in the outer part were identified Ca, P, Fe, Mn, Sr and Cu. The Ca ratio of the outer part by inner part of the analyzed shells is around of 1, as expected, because Ca is the main compound of mollusks shells. The ratio of P, Fe, Mn, and Sr to the Ca were constant in all analyzed shells, being close to 0.015. The ratio Cu/Ca varied among the shells, showing that this mollusk is sensitive to concentration of this element in the aquatic environment.

  14. Leeches as Sensor-bioindicators of River Contamination by PCBs

    Science.gov (United States)

    Macova, Stanislava; Harustiakova, Danka; Kolarova, Jitka; Machova, Jana; Zlabek, Vladimir; Vykusova, Blanka; Randak, Tomas; Velisek, Josef; Poleszczuk, Gorzyslaw; Hajslova, Jana; Pulkrabova, Jana; Svobodova, Zdenka

    2009-01-01

    The aim of the study was to evaluate the use of leeches of the genus Erpobdella as a means of assessing polychlorinated biphenyl contamination of watercourses. The River Skalice, heavily contaminated with PCBs, was selected as a model. The source of contamination was a road gravel processing factory in Rožmitál pod Třemšínem from which an estimated 1 metric ton of PCBs leaked in 1986. Levels of PCB were measured in leeches collected between 1992 to 2003 from 11 sites covering about 50 km of the river (the first sampling site upstream to the source of contamination and 10 sites downstream). The PCB indicator congeners IUPA no. 28, 52, 101, 118, 138, 153, and 180 were measured. Levels were highest at the four sampling sites nearest the source of pollution. The highest values of PCB congeners were found in 1992. PCB content decreased from 1992 to 2003 and with distance from the source. The study indicated that leeches of the genus Erpobdella are a suitable bioindicator of contamination in the surface layer of river sediments. PMID:22573988

  15. Comparison of fish and macroinvertebrates as bioindicators of Neotropical streams.

    Science.gov (United States)

    Ruaro, Renata; Gubiani, Éder André; Cunico, Almir Manoel; Moretto, Yara; Piana, Pitágoras Augusto

    2016-01-01

    The biomonitoring of aquatic ecosystems in developing countries faces several limitations, especially related to gathering resources. The present study aimed at comparing the responses of fish and benthic macroinvertebrates to environmental change, to identify which group best indicates the differences between reference and impacted streams in southern Brazil. We determined reference and impacted sites based on physical and chemical variables of the water. For the analysis and comparison of biological responses, we calculated 22 metrics and submitted them to a discriminant analysis. We selected from this analysis only six metrics, which showed that the two studied assemblages respond differently to environmental change. A larger number of metrics were selected for macroinvertebrates than for fish in the separate analysis. The metrics selected for macroinvertebrates in the pooled analysis (i.e., fish and macroinvertebrates together) were different from those selected in the separate analysis for macroinvertebrates alone. However, the metrics selected for fish in the pooled analysis were the same selected in the separate analysis for fish alone. The macroinvertebrate assemblage was more effective for distinguishing reference from impacted sites. We suggest the use of macroinvertebrates as bioindicators of Neotropical streams, especially in situations in which time and money are short.

  16. Phenolic acids as bioindicators of fly ash deposit revegetation

    Energy Technology Data Exchange (ETDEWEB)

    L. Djurdjevic; M. Mitrovic; P. Pavlovic; G. Gajic; O. Kostic [Institute for Biological Research ' Sinisa Stankovic,' Belgrade (Serbia and Montenegro). Department of Ecology

    2006-05-15

    The floristic composition, the abundance, and the cover of pioneer plant species of spontaneously formed plant communities and the content of total phenolics and phenolic acids, as humus constituents, of an ash deposit after 7 years of recultivation were studied. The restoration of both the soil and the vegetation on the ash deposits of the 'Nikola Tesla-A' thermoelectric power plant in Obrenovac (Serbia) is an extremely slow process. Unfavorable physical and chemical characteristics, the toxicity of fly ash, and extreme microclimatic conditions prevented the development of compact plant cover. The abundance and cover of plants increased from the central part of the deposit towards its edges. Festuca rubra L., Crepis setosa Hall., Erigeron canadensis L., Cirsium arvense (L.) Scop., Calamagrostis epigeios (L.) Roth., and Tamarix gallica L. were the most abundant species, thus giving the highest cover. Humus generated during the decomposition process of plant remains represents a completely new product absent in the ash as the starting material. The amount of total phenolics and phenolic acids in fly ash increased from the center of the deposit towards its edges in correlation with the increase in plant abundance and cover. The presence of phenolic acids indicates the ongoing process of humus formation in the ash, in which the most abundant pioneer plants of spontaneously formed plant communities play the main role. Phenolic compounds can serve as reliable bioindicators in an assessment of the success of the recultivation process of thermoelectric power plants' ash deposits.

  17. Hibiscus sabdariffa L. water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway.

    Science.gov (United States)

    Kim, Jin-Kyung; So, Hongseob; Youn, Myung-Ja; Kim, Hyung-Jin; Kim, Yunha; Park, Channy; Kim, Se-Jin; Ha, Young-Ae; Chai, Kyu-Yun; Kim, Shin-Moo; Kim, Ki-Young; Park, Raekil

    2007-11-01

    Hibiscus sabdariffa L., a tropical beverage material and medical herb, is used commonly as in folk medicines against hypertension, pyrexia, inflammation, liver disorders, and obesity. This report was designed to investigate the inhibitory mechanisms of hibiscus extract on adipocyte differentiation in 3T3-L1 preadipocytes. The possible inhibitory pathways that regulate the adipocyte differentiation contain the adipogenic transcription factors, C/EBPalpha and PPARgamma, PI3-kinase, and MAPK pathway. In this study, we examined whether hibiscus extract affected the adipogenesis via these three pathways. To differentiate preadipocyte in adipocyte, confluent 3T3-L1 preadipocytes were treated with the hormone mixture including isobutylmethylxanthine, dexamethasone, and insulin (MDI). Hibiscus extract inhibited significantly the lipid droplet accumulation by MDI in a dose-dependent manner and attenuated dramatically the protein and mRNA expressions of adipogenic transcriptional factors, C/EBPalpha and PPARgamma, during adipogenesis. The increase of phosphorylation and expression of PI3-K/Akt during adipocytic differentiation was markedly inhibited by treatment with hibiscus extract or PI3-K inhibitors. Furthermore, the phosphorylation and expression of MEK-1/ERK known to regulate the early phase of adipogenesis were clearly decreased with the addition of hibiscus extract. Taken together, this report suggests that hibiscus extract inhibits the adipocyte differentiation through the modulation of PI3-K/Akt and ERK pathway that play pivotal roles during adipogenesis.

  18. The Effects of Cold Stress on Photosynthesis in Hibiscus Plants

    Science.gov (United States)

    Paredes, Miriam; Quiles, María José

    2015-01-01

    The present work studies the effects of cold on photosynthesis, as well as the involvement in the chilling stress of chlororespiratory enzymes and ferredoxin-mediated cyclic electron flow, in illuminated plants of Hibiscus rosa-sinensis. Plants were sensitive to cold stress, as indicated by a reduction in the photochemistry efficiency of PSII and in the capacity for electron transport. However, the susceptibility of leaves to cold may be modified by root temperature. When the stem, but not roots, was chilled, the quantum yield of PSII and the relative electron transport rates were much lower than when the whole plant, root and stem, was chilled at 10°C. Additionally, when the whole plant was cooled, both the activity of electron donation by NADPH and ferredoxin to plastoquinone and the amount of PGR5 polypeptide, an essential component of the cyclic electron flow around PSI, increased, suggesting that in these conditions cyclic electron flow helps protect photosystems. However, when the stem, but not the root, was cooled cyclic electron flow did not increase and PSII was damaged as a result of insufficient dissipation of the excess light energy. In contrast, the chlororespiratory enzymes (NDH complex and PTOX) remained similar to control when the whole plant was cooled, but increased when only the stem was cooled, suggesting the involvement of chlororespiration in the response to chilling stress when other pathways, such as cyclic electron flow around PSI, are insufficient to protect PSII. PMID:26360248

  19. Fatty Acid Composition of Hibiscus trionum L. (Malvaceae

    Directory of Open Access Journals (Sweden)

    Ceyda Sibel Kılıç

    2011-01-01

    Full Text Available The genus Hibiscus plants have different uses, some are used as foods (H. esculenta L., and some species as remedy in traditional medicine (H. sabdariffa L. as well as a colorant for herbal teas. The only species that grows naturally in Turkey is H. trionum L. The plant especially infests soy and corn fields, and therefore it is considered to be a noxious weed. The plant is also found to be a host for Potato Virus Y (PVY and Tomato Spotted Wilt Virus (TSWV. Infestation of the crop fields by this plant shows that it germinates easily. Since it produces many seeds, distribution of the plant is also quiet easy. Though, seed oil yield is low (4.7% since linoleic acid composition of the seed oil is quiet high (67,5%, it can still be used as a source of unsaturated fatty acids. The other major fatty acids are palmitic, oleic and stearic acids in the studied seed oil .

  20. Hibiscus fiber carbon for fuel cell device material

    International Nuclear Information System (INIS)

    Nanik Indayaningsih; Anne Zulfia; Dedi Priadi; Suprapedi

    2010-01-01

    The objective of this research is carbon of hibiscus fibers for the application as basic material of fuel cell device. The carbon is made using a pyrolysis process in inert gas (nitrogen) for 1 hour at temperature of 500 °C, 700 °C and 900 °C. The X-Ray Diffractometer (XRD), Scanning Electron Microscope (SEM) and Impedance-Capacitance-Resistance-meter are used to find out the microstructure, morphology and electrical properties respectively. The results of the experiment showed that the carbon had a structure of amorphous, and as the semiconductor material the electrical conductivity was 5 x 10 -5 S.cm -1 to 4.9 x 10 -5 S.cm -1 increasing in accordance with the pyrolysis temperature. The morphology resembled to plaited mats constructed by porous fibers having width of 50 µm to 300 µm, thickness of 25 µm to 35 µm, and the porous size of 0.5 µm to 5 µm. This morphology enables carbon to be applied as a candidate for a basic material of the Proton Exchange Membrane Fuel Cell. (author)

  1. Fullerene-Based Symmetry in Hibiscus rosa-sinensis Pollen

    Science.gov (United States)

    Andrade, Kleber; Guerra, Sara; Debut, Alexis

    2014-01-01

    The fullerene molecule belongs to the so-called super materials. The compound is interesting due to its spherical configuration where atoms occupy positions forming a mechanically stable structure. We first demonstrate that pollen of Hibiscus rosa-sinensis has a strong symmetry regarding the distribution of its spines over the spherical grain. These spines form spherical hexagons and pentagons. The distance between atoms in fullerene is explained applying principles of flat, spherical, and spatial geometry, based on Euclid’s “Elements” book, as well as logic algorithms. Measurements of the pollen grain take into account that the true spine lengths, and consequently the real distances between them, are measured to the periphery of each grain. Algorithms are developed to recover the spatial effects lost in 2D photos. There is a clear correspondence between the position of atoms in the fullerene molecule and the position of spines in the pollen grain. In the fullerene the separation gives the idea of equal length bonds which implies perfectly distributed electron clouds while in the pollen grain we suggest that the spines being equally spaced carry an electrical charge originating in forces involved in the pollination process. PMID:25003375

  2. Okra (Hibiscus esculentus) seed oil for biodiesel production

    Energy Technology Data Exchange (ETDEWEB)

    Anwar, Farooq; Nadeem, Muhammad [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Rashid, Umer [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Department of Industrial Chemistry, Government College University, Faisalabad 38000 (Pakistan); Ashraf, Muhammad [Department of Botany, University of Agriculture, Faisalabad 38040 (Pakistan)

    2010-03-15

    Biodiesel was derived from okra (Hibiscus esculentus) seed oil by methanol-induced transesterification using an alkali catalyst. Transesterification of the tested okra seed oil under optimum conditions: 7:1 methanol to oil molar ratio, 1.00% (w/w) NaOCH{sub 3} catalyst, temperature 65 C and 600 rpm agitation intensity exhibited 96.8% of okra oil methyl esters (OOMEs) yield. The OOMEs/biodiesel produced was analyzed by GC/MS, which showed that it mainly consisted of four fatty acids: linoleic (30.31%), palmitic (30.23%), oleic (29.09%) and stearic (4.93%). A small amount of 2-octyl cyclopropaneoctanoic acid with contribution 1.92% was also established. Fuel properties of OOMEs such as density, kinematic viscosity, cetane number, oxidative stability, lubricity, flash point, cold flow properties, sulfur contents and acid value were comparable with those of ASTM D 6751 and EN 14214, where applicable. It was concluded that okra seed oil is an acceptable feedstock for biodiesel production. (author)

  3. Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa.

    Science.gov (United States)

    Sindi, Heba A; Marshall, Lisa J; Morgan, Michael R A

    2014-12-01

    Hibiscus sabdariffa extracts have attracted attention because of potentially useful bioactivity. However, there have been no systematic studies of extraction efficiencies of H. sabdariffa. The nature of extracts used in different studies has varied considerably, making comparisons difficult. Therefore, a systematic study of extracts of H. sabdariffa made with different solvents was carried out using water, methanol, ethyl acetate and hexane in the presence/absence of formic acid, using different extraction times and temperatures. The extracts were analysed for total polyphenol content, antioxidant capacity using DPPH, FRAP and TEAC assays, and specific anthocyanins were determined using HPLC and LC-MS. The results showed the highest antioxidant capacities were obtained by extracting using water, with or without formic acid, for 10 min at 100°C. These extracts provided the highest concentrations of cyanidin 3-sambubioside and delphinidin 3-sambubioside. It will be important to use extraction conditions giving optimal extraction efficiencies for subsequent bioactivity experiments. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  5. The Effects of Cold Stress on Photosynthesis in Hibiscus Plants.

    Science.gov (United States)

    Paredes, Miriam; Quiles, María José

    2015-01-01

    The present work studies the effects of cold on photosynthesis, as well as the involvement in the chilling stress of chlororespiratory enzymes and ferredoxin-mediated cyclic electron flow, in illuminated plants of Hibiscus rosa-sinensis. Plants were sensitive to cold stress, as indicated by a reduction in the photochemistry efficiency of PSII and in the capacity for electron transport. However, the susceptibility of leaves to cold may be modified by root temperature. When the stem, but not roots, was chilled, the quantum yield of PSII and the relative electron transport rates were much lower than when the whole plant, root and stem, was chilled at 10°C. Additionally, when the whole plant was cooled, both the activity of electron donation by NADPH and ferredoxin to plastoquinone and the amount of PGR5 polypeptide, an essential component of the cyclic electron flow around PSI, increased, suggesting that in these conditions cyclic electron flow helps protect photosystems. However, when the stem, but not the root, was cooled cyclic electron flow did not increase and PSII was damaged as a result of insufficient dissipation of the excess light energy. In contrast, the chlororespiratory enzymes (NDH complex and PTOX) remained similar to control when the whole plant was cooled, but increased when only the stem was cooled, suggesting the involvement of chlororespiration in the response to chilling stress when other pathways, such as cyclic electron flow around PSI, are insufficient to protect PSII.

  6. Wound Healing Potential of Formulated Extract from Hibiscus Sabdariffa Calyx

    Science.gov (United States)

    Builders, P. F.; Kabele-Toge, B.; Builders, M.; Chindo, B. A.; Anwunobi, Patricia A.; Isimi, Yetunde C.

    2013-01-01

    Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (Psabdariffa extract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin. PMID:23901160

  7. Physicochemical properties and antimicrobial activity of Roselle (Hibiscus sabdariffa L.).

    Science.gov (United States)

    Jung, EunKyung; Kim, YoungJun; Joo, Nami

    2013-12-01

    The therapeutic action of a plant depends on its chemical constituents. In this study, experiments were carried out in order to evaluate the effect of extraction conditions on the antioxidative and antimicrobial activities of Roselle (Hibiscus sabdariffa L.). Roselle was found to be rich in malic acid, anthocyanins, ascorbic acid and minerals, especially Ca and Fe, but low in glucose. More than 18 volatile compounds were identified by gas chromatography and gas chromatography-mass spectrometry. This herb, which is rich in phenolic compounds and displays DPPH radical scavenging activity, could be a good source of natural antioxidants. The antimicrobial activity of the Roselle water and ethanol extracts was tested with Bacillus subtilis (ATCC6633), Staphylococcus aureus (ATCC6538) and Escherichia coli (ATCC 8739). The inhibition of the Roselle ethanol extract against B. subtilis and S. aureus was slightly higher than that of water extract but this difference was not significant. However, E. coli was strongly inhibited by the Roselle water extract at concentrations of 25 and 50 mg mL(-1) as determined by a paper disc method. The obtained results indicated that antioxidant and antimicrobial activity was related to different methods of extraction and Roselle extracts could be a source of therapeutically useful products. © 2013 Society of Chemical Industry.

  8. F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil.

    Science.gov (United States)

    Hong, J W; Fomina, M; Gadd, G M

    2010-08-01

    This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm. By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination. F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination. These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.

  9. Toxicity studies of the water extract from the calyces of Hibiscus ...

    African Journals Online (AJOL)

    Acute and chronic toxicities of the water extract from calyces of Hibiscus sabdariffa were studied in male and female rats. After 14 days of a single oral administration of test substance 5,000 mg/kg body weight, measurement of the body and organ weights, necropsy and health monitoring were performed. No signs and ...

  10. Kenaf (Hibiscus cannabinus L.) impact on post-germination seedling growth

    Science.gov (United States)

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the post-germination growth of five plant species...

  11. Awareness and utilization of “Sobo” ( Hibiscus sabdariffa-roselle ) by ...

    African Journals Online (AJOL)

    Hibiscus sabdariffa (Roselle) is known for its numerous health and medicinal benefits. “Sobo” is an indigenous non-alcoholic drink made from hot extract of Roselle calyces and consumed in Savannah and semi-arid region of Africa. This study assessed awareness and utilization of sobo among farm families in Aniocha ...

  12. Molecular characterization of ferulate 5-hydroxylase gene from kenaf (Hibiscus cannabinus L.)

    Science.gov (United States)

    The purpose of this research was to clone and characterize the expression pattern of a kenaf (Hibiscus cannabinus L.) F5H gene that encodes ferulate 5-hydroxylase in the phenylpropanoid pathway. Kenaf is well known as a fast growing dicotyledonous plant, which makes it a valuable biomass plant. The ...

  13. Effects of Hibiscus sabdariffa L. on serum lipids: a systematic review and meta-analysis.

    Science.gov (United States)

    Aziz, Zoriah; Wong, Su Yuen; Chong, Nyuk Jet

    2013-11-25

    Prevention of cardiovascular disease by modifying its major risk factors, including serum cholesterol levels, is an important strategy. Hibiscus sabdariffa L. has been promoted for reducing cholesterol levels, but its reported impact on cholesterol levels has been inconsistent. The study aimed to assess systematically the evidence and quality of current research on the effect of Hibiscus sabdariffa L. on blood lipids and its adverse effects. Electronic databases were searched up to June 2013 for relevant randomised controlled trials (RCTs). Journals and conference proceedings were also searched. The quality of the selected trials was assessed using the Cochrane Risk of Bias Assessment Tool. The efficacy results of similar studies were pooled if they used the same comparator. Outcomes examined were levels of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglycerides. Six studies involving 474 subjects met our inclusion criteria. These studies varied in terms of the types of interventions, comparators used, and duration of trials. Overall, Hibiscus sabdariffa L. did not produce any significant effect on any of the outcomes examined, when compared with placebo, black tea or diet. With short-term use it is well tolerated. The available evidence from RCTs does not support the efficacy of Hibiscus sabdariffa L. in lowering serum lipids. Further rigorously designed trials with larger sample sizes are warranted to confirm the effects of HS on serum lipids. © 2013 Elsevier Ireland Ltd. All rights reserved.

  14. Effect of methanolic extract of Hibiscus sabdariffa in ethanol-induced ...

    African Journals Online (AJOL)

    The objective of this study was to evaluate the activity of Hibiscus sabdariffa on the liver of rats following repeated administration of ethanol. Hepatotoxicity was induced on the rats using ethanol and the levels of serum enzymes such as serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase ...

  15. Effect of rosella ( Hibiscus sabdariffa L ) extract on glutathione-S ...

    African Journals Online (AJOL)

    Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-trasferase (GST) activity and its hepatoprotective effect. Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and ...

  16. ‘Hapa White’, 'Hapa Pink', and 'Hapa Red' interspecific hybrid hibiscus cultivars

    Science.gov (United States)

    Hibiscus mutabilis, also known as confederate rose, is native to southeastern China, but it is also grown as an ornamental throughout the southeastern United States and is hardy in USDA zone 7 to 9. It is popular for its large, soft, gray-green foliage during the summer, and large, showy flowers pro...

  17. Complete nucleotide sequence of Hibiscus infecting Cilevirus Florida isolate and its relationship with closely associated Cileviruses

    Science.gov (United States)

    The complete nucleotide sequence of a recently discovered Florida (FL) isolate of Hibiscus infecting Cilevirus (HiCV) was determined by Sanger sequencing. The movement- and coat- protein gene sequences of the HiCV-FL isolate are more divergent than other genes of the previously sequenced HiCV-HA (Ha...

  18. Cytotoxic Activity of the Leaf and Stem Extracts of Hibiscus rosa ...

    African Journals Online (AJOL)

    activity of Albizzia Lebbeck, Hibiscus rosa sinensis and Butea monosperma in experimental animals. J. Ethanopharmacol. 2000; 71: 65-75. 5. Ali M, Ansari SH. Hair Care and Herbal Drugs. J Nat Prod. 1997; 13: 3-5. 6. Kurup PNV, Ramdas VNK, Joshi P. Handbook of Medicinal. Plants. New Dehli; 1997. 86p. 7. Freshney RI.

  19. Z-scan and optical limiting properties of Hibiscus sabdariffa dye

    CSIR Research Space (South Africa)

    Maaza, M

    2014-12-01

    Full Text Available The intensity-dependent refractive index n(sub2) and the nonlinear susceptibility X(sup3) of Hibiscus Sabdariffa dye solutions in the nanosecond regime at 532 nm are reported. More presicely, the variation of n(sub 2), ß, and real and imaginary...

  20. Phytomining of heavy metals from soil by Hibiscus radiatus using phytoremediationtechnology (Part-2)

    Science.gov (United States)

    Panchal, K. J.; Subramanian, R. B.; Gohil, T. P.

    2017-12-01

    Metal ions are not only valuable intermediates in metal extraction, but also important raw materials fortechnical applications. They possess some unique but, identical physical and chemical properties, whichmake them useful probes of low temperature geochemical reactions. Heavy metals are natural constituentsof the earth's crust, but indiscriminate human activities have drastically altered their geochemical cyclesand biochemical balance. Metal concentration in soil typically ranges from less than one to as high as100,000 mg/kg. Heavy metal contaminations of land resources continue to be the focus of numerousenvironmental studies and attract a great deal of attention worldwide. This is attributed to nobiodegradabilityand persistence of heavy metals in soils. Prolonged exposure to heavy metals such ascadmium, copper, lead, nickel, and zinc can cause deleterious health effects in humans. Complexation,separation, and removal of metal ions have become increasingly attractive areas of research and have ledto new technical developments like phytoremediation that has numerous biotechnological implications ofunderstanding of plant metal accumulation. Hibiscus radiatus is newly identified as a potential heavymetal hypreaccumulator. In this study Hibiscus radiatus was subjected for in vitro heavy metalaccumulation, to explore the accumulation pattern of four heavy metals viz Cadmium, Lead, Nickel andZinc in various parts of Hibiscus radiatus plant parts. Translocation of metals in Hibiscus radiatus plant parts from soil makes this plant an eligible candidate to remove heavy metals from soil.

  1. Validation of two pheromonal compounds for monitoring pink hibiscus mealybug in Mexico

    Science.gov (United States)

    A Pink Hibiscus Mealybug (Macconelicoccus hirsutus (Green)) was detected, in 2004, in Valle de Banderas, at municipalities Bahía de Banderas, Nayarit, and Puerto Vallarta, Jalisco, México, affecting fruit trees, native and ornamental plants. This pest insect is native to Asia and Australia, and has ...

  2. Effet de l'incorporation de graines d' Hibiscus Sabdariffa (Oseille de ...

    African Journals Online (AJOL)

    Effet de l'incorporation de graines d' Hibiscus Sabdariffa (Oseille de Guinée) dans l'alimentation sur quelques paramètres d'ingestion et de croissance du poulet en aviculture traditionnelle améliorée au Burkina Faso.

  3. Cytotoxic Activity of the Leaf and Stem Extracts of Hibiscus rosa ...

    African Journals Online (AJOL)

    Methods: The crude petroleum ether, ethyl acetate and methanol extracts of the leaf and stem of Hibiscus rosa sinensis were prepared using cold extraction method. The in vitro cytotoxic activity of the extracts (20 - 100 μg/ml) was evaluated on leukaemic cancer cell line (K-562) and Mardin-Darby kidney cell line (MDBK) ...

  4. Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S ...

    African Journals Online (AJOL)

    stress caused by free radicals produced from pollutants and foods. Keywords: Hibiscus sabdariffa, Rosella, Glutamate pyruvate ... materials. They are also found in cigarette smoke, grilled food and motor vehicle exhaust fumes [3,4]. .... result was captured by photographing the ethidium bromide flourescense in a dark room.

  5. The effect of Hibiscus sabdariffa calyx extract on cisplatin-induced ...

    African Journals Online (AJOL)

    JTEkanem

    2008-12-14

    Dec 14, 2008 ... The effect of Hibiscus sabdariffa calyx extract on cisplatin-induced tissue damage was studied. A total of twenty rats were used for the study and split into four groups of five rats per group-group I, II, III, and IV. Tissue damage was induced in rats of groups II, III, and IV by a single intraperitoneal administration ...

  6. Micro-element contents of roselle ( Hibiscus sabdariffa L.) at different ...

    African Journals Online (AJOL)

    In the western Sahel, leaves of Roselle (Hibiscus sabdariffa) have considerable economic importance because of their nutritional and medical uses. These plant organs are used to supplement nutrients provided by cereals such as millet and sorghum. However, there is a lack of information on the nutrient composition of ...

  7. Comparative Analysis of the Properties of Acid-Base Indicator of Rose (Rosa setigera, Allamanda (Allamanda cathartica, and Hibiscus (Hibiscus rosa-sinensis Flowers

    Directory of Open Access Journals (Sweden)

    Stanley I. R. Okoduwa

    2015-01-01

    Full Text Available The need to develop effective alternative for synthetic indicators is the demand of present-day chemistry. The acid-base indicator properties of Rose (Rosa setigera, Allamanda (Allamanda cathartica, and Hibiscus (Hibiscus rosa-sinensis flowers were examined. Colour pigments were extracted from the flowers via cold and solvent extraction using soxhlet extractor. The pH value of the extracts with wavelengths of absorption was determined using ultraviolet spectrophotometer. From the results obtained, all the extracts exhibited sharp contrast between their colours in acid and base. Their pH was found to be 5.5 for cold extract of Rose and 5.6 for solvent extraction, 5.24 for cold extract of a Hibiscus and 6.52 for solvent extraction, 5.35 for cold extract of Allamanda, and 5.45 for solvent extraction. The maximum wavelengths of absorption obtained for all the extract fall within the visible region of electromagnetic spectrum. These values are almost similar to that obtained from synthetic indicators. It is on these bases that we concluded that natural indicators could be an excellent replacement for synthetic indicators since they are cheap, readily available, simple to extract, not toxic, user and environmentally friendly.

  8. Seabird eggs as bioindicators of chemical contamination in Chile

    International Nuclear Information System (INIS)

    Cifuentes, Jacqueline Munoz; Becker, Peter H.; Sommer, Ute; Pacheco, Patricia; Schlatter, Roberto

    2003-01-01

    Seabird eggs are proposed as biomonitors of chemical contamination in Chile. - Seabird eggs were used as bioindicators of chemical contamination in Chile. Brown-hooded Gull (Larus maculipennis), Kelp Gull (Larus dominicanus), Trudeau's Tern (Sterna trudeaui), Neotropic Cormorant (Phalacrocorax brasilianus), and Pink-footed Shearwater (Puffinus creatopus) eggs were sampled at different breeding sites during the 1990s. Mercury and organochlorines (PCBs, DDT, HCB, HCH, and PCP) were quantified to reveal the interspecific differences, spatial and temporal trends in contamination levels. Trudeau's Tern displayed the highest levels of mercury (486 ng g -1 wet weight). The highest ΣDDT concentrations were measured in Brown-hooded Gulls (726 ng g -1 ). PCB levels were similar among the species (102-236 ng g -1 ), but the composition of the PCB mixture was different in Pink-footed Shearwaters. With the exception of the Brown-hooded Gull, all species studied presented similar and low levels of organochlorines (ΣOHa). Residues of PCB and related compounds were not detected in any of the seabird eggs analyzed in Chile. Geographical variation was low, although levels of industrial chemicals were slightly higher in eggs from Concepcion Bay, and agricultural chemicals in eggs from Valdivia. Also interannual variation was low, but some evidence was found of decreasing levels in gull eggs throughout the time of the study. The causes of the low levels and small variability in space and time of environmental chemicals in Chilean seabirds are discussed. We propose the use of seabirds in future monitoring of the development of chemical contamination in Chile

  9. Biochar–macrofauna interplay: Searching for new bioindicators

    Energy Technology Data Exchange (ETDEWEB)

    Castracani, C., E-mail: cristina.castracani@unipr.it [Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/a, 43124 Parma (Italy); Maienza, A., E-mail: a.maienza@ibimet.cnr.it [Institute of Biometeorology, National Research Council (IBIMET-CNR), Via G. Caproni 8, 50145 Firenze (Italy); Grasso, D.A., E-mail: donato.grasso@unipr.it [Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/a, 43124 Parma (Italy); Genesio, L., E-mail: l.genesio@ibimet.cnr.it [Institute of Biometeorology, National Research Council (IBIMET-CNR), Via G. Caproni 8, 50145 Firenze (Italy); Malcevschi, A., E-mail: alessio.malcevschi@unipr.it [Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/a, 43124 Parma (Italy); Miglietta, F., E-mail: f.miglietta@ibimet.cnr.it [Institute of Biometeorology, National Research Council (IBIMET-CNR), Via G. Caproni 8, 50145 Firenze (Italy); Vaccari, F.P., E-mail: f.vaccari@ibimet.cnr.it [Institute of Biometeorology, National Research Council (IBIMET-CNR), Via G. Caproni 8, 50145 Firenze (Italy); Mori, A., E-mail: alessandra.mori@unipr.it [Department of Life Sciences, University of Parma, Parco Area delle Scienze 11/a, 43124 Parma (Italy)

    2015-12-01

    Biochar incorporation in agricultural soils has been proposed as a climate change mitigation strategy and has proved to substantially increase crop productivity via physical, chemical and biological mechanisms. The changes induced in soil properties are known to have a direct impact on soil ecosystem with consequences for soil biota community that, in turn, can influence biochar aging in soil. Despite several studies investigated in the interplay between biochar and soil microbiology, there is a clear lack of information on groups that live in the most superficial ground layers: soil meso and macro fauna. These groups are of great importance if we consider that biochar application should ideally be located in the soil's surface layer (0–30 cm). Our study is the first attempt to investigate the interactions between biochar soil amendments and aboveground soil macro-meso fauna in a field crop. This was made setting-up a randomized-block experiment on a processing tomato crop in northern Italy, using three different biochar types and periodically monitoring soil parameters and fauna abundances along the crop growing cycle in summer 2013. Results show that the impact of biochar application on soil fauna as a whole is small when compared to that of agricultural management, suggesting that this amendment does not have short-term ecological interferences. Nevertheless, ants exhibited variations in abundances and distribution connected to properties of amended soils such as temperature, pH and humidity, proving that they can be effectively used as a target group in the study of interactions between biochar and soil biota. - Highlights: • We use a field study to evaluate the effects of biochar on aboveground fauna. • We focus on ants as useful bioindicators for biochar environmental safety. • Biochar has less impact on soil fauna compared to agricultural management. • Ant distribution can be connected to properties of amended soils.

  10. Biomarkers in Ruditapes decussatus: a potential bioindicator species.

    Science.gov (United States)

    Bebianno, M J; Géret, F; Hoarau, P; Serafim, M A; Coelho, M R; Gnassia-Barelli, M; Roméo, M

    2004-01-01

    The clam Ruditapes decussatus is distributed worldwide and due to its ecological and economical interest has been proposed as a bioindicator in areas where mussels are not available. The accumulation of several anthropogenic compounds in their tissues suggests that they possess mechanisms that allow them to cope with the toxic effects of these contaminants. Besides pollutant uptake, the use of biomarkers is pointed out in this paper since it is a promising approach to monitor the effect of these contaminants in the marine environment. Biomarkers complement the information of the direct chemical characterization of different types of contaminants. Therefore, the aim of this paper is to review the role of several biomarkers: (metallothioneins (MT), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx) (total and selenium-dependent), lipid peroxidation (measured as MDA, one of the final products of lipid peroxidation), glutathione S-transferase (GST) and acetylcholinesterase (AChE), measured in different tissues of the clam R. decussatus, in laboratory conditions and under various environmental stresses, in two ecosystems (Ria Formosa lagoon- Portugal) and Bizerta lagoon (Tunisia) in a perspective of a multibiomarker approach to assess environmental changes. Experiment and field studies are in good agreement since MT levels, especially in the gills, the first target tissue of these contaminants, can be used as biomarker of exposure to Cd. GPx and MDA may also be determined in this respect. AChE activity is inhibited by pesticide and, to a less extent, by metal exposure in the gills and whole soft body of clams. However, the induction of GST isoforms experimentally demonstrated is not observed in the field because only global GST activity was determined. The whole set of results opens new research perspectives for the use of this species to assess the effect of mixtures of pollutants in the aquatic environment.

  11. Biochar–macrofauna interplay: Searching for new bioindicators

    International Nuclear Information System (INIS)

    Castracani, C.; Maienza, A.; Grasso, D.A.; Genesio, L.; Malcevschi, A.; Miglietta, F.; Vaccari, F.P.; Mori, A.

    2015-01-01

    Biochar incorporation in agricultural soils has been proposed as a climate change mitigation strategy and has proved to substantially increase crop productivity via physical, chemical and biological mechanisms. The changes induced in soil properties are known to have a direct impact on soil ecosystem with consequences for soil biota community that, in turn, can influence biochar aging in soil. Despite several studies investigated in the interplay between biochar and soil microbiology, there is a clear lack of information on groups that live in the most superficial ground layers: soil meso and macro fauna. These groups are of great importance if we consider that biochar application should ideally be located in the soil's surface layer (0–30 cm). Our study is the first attempt to investigate the interactions between biochar soil amendments and aboveground soil macro-meso fauna in a field crop. This was made setting-up a randomized-block experiment on a processing tomato crop in northern Italy, using three different biochar types and periodically monitoring soil parameters and fauna abundances along the crop growing cycle in summer 2013. Results show that the impact of biochar application on soil fauna as a whole is small when compared to that of agricultural management, suggesting that this amendment does not have short-term ecological interferences. Nevertheless, ants exhibited variations in abundances and distribution connected to properties of amended soils such as temperature, pH and humidity, proving that they can be effectively used as a target group in the study of interactions between biochar and soil biota. - Highlights: • We use a field study to evaluate the effects of biochar on aboveground fauna. • We focus on ants as useful bioindicators for biochar environmental safety. • Biochar has less impact on soil fauna compared to agricultural management. • Ant distribution can be connected to properties of amended soils

  12. Phenolic acids as bioindicators of fly ash deposit revegetation.

    Science.gov (United States)

    Djurdjević, L; Mitrović, M; Pavlović, P; Gajić, G; Kostić, O

    2006-05-01

    The floristic composition, the abundance, and the cover of pioneer plant species of spontaneously formed plant communities and the content of total phenolics and phenolic acids, as humus constituents, of an ash deposit after 7 years of recultivation were studied. The restoration of both the soil and the vegetation on the ash deposits of the "Nikola Tesla-A" thermoelectric power plant in Obrenovac (Serbia) is an extremely slow process. Unfavorable physical and chemical characteristics, the toxicity of fly ash, and extreme microclimatic conditions prevented the development of compact plant cover. The abundance and cover of plants increased from the central part of the deposit towards its edges (ranging from 1-80%). Festuca rubra L., Crepis setosa Hall., Erigeron canadensis L., Cirsium arvense (L.) Scop., Calamagrostis epigeios (L.) Roth., and Tamarix gallica L. were the most abundant species, thus giving the highest cover. Humus generated during the decomposition process of plant remains represents a completely new product absent in the ash as the starting material. The amount of total phenolics and phenolic acids (38.07-185.16 microg/g of total phenolics and 4.12-27.28 microg/g of phenolic acids) in fly ash increased from the center of the deposit towards its edges in correlation with the increase in plant abundance and cover. Ash samples contained high amounts of ferulic, vanillic, and p-coumaric acid, while the content of both p-hydroxybenzoic and syringic acid was relatively low. The presence of phenolic acids indicates the ongoing process of humus formation in the ash, in which the most abundant pioneer plants of spontaneously formed plant communities play the main role. Phenolic compounds can serve as reliable bioindicators in an assessment of the success of the recultivation process of thermoelectric power plants' ash deposits.

  13. Honey as a bioindicator of environment contamination with caesium

    International Nuclear Information System (INIS)

    Barisic, D.; Lovrencic, I.; Orescanin, V.; Kezic, N.; Bubalo, D.; Popijac, M.; Volner, M.

    2005-01-01

    Collecting nectar and pollen, bees cover area of approximately 20 km 2 . Honey from a beehive represents a composite sample collected from several hundreds of millions of points and is probably one of the most representative random samples possible to collect in the environment. Therefore, information contained in honey gives good reflection of an average environment condition considering bioavailable elements and/or chemical compounds. Results of measured 1 37C s activities in different types of honey collected on the area of Republic of Croatia in period from 2000 to 2003 are given in this paper. The activity of 1 37C s is measured with gamma spectrometric method and the types of honey are defined on the basis of pollen analysis and from measuring electro conductivity. More than 15 years after the accident in Chernobyl, it is still possible to monitor 1 37C s activity in several types of honey. The greatest 1 37C s activities are detected in pure fir-tree and spruce honey-dew honey (15.7 ± 5.6 Bq/kg), mixed honey containing honey-dew (7.4 ± 3.9 Bq/kg) and in pure chestnut honey (4.5 ± 2.4 Bq/kg). On the other side, 1 37C s has not been found in any of the samples of pure lime- and locust-tree honeys, while in the meadow honey it has been detected only twice. Considering that measured activities of 1 37C s in honey correspond with the levels of contamination of particular areas, coniferous honey-dew honeys, as well as pure chestnut honey, can be used as bioindicators in monitoring the environment contaminated with 1 37C s.(author)

  14. In vivo pharmacodynamic and pharmacokinetic interactions of Hibiscus sabdariffa calyces extracts with simvastatin.

    Science.gov (United States)

    Showande, S J; Adegbolagun, O M; Igbinoba, S I; Fakeye, T O

    2017-12-01

    Increasing number of patients use herbs with their medications. Such practice may result in beneficial or harmful herb-drug interactions. A recent survey reported that some participants co-administered Hibiscus sabdariffa, a widely used beverage, or tea, with their antihyperlipidaemic medications. This study therefore evaluated the effect of concomitant administration of Hibiscus sabdariffa calyces' extracts with simvastatin on hyperlipidaemia and pharmacokinetics of the drug in vivo. Factorial experimental designs were used to evaluate the comparative effectiveness and interactions between simvastatin and aqueous extract of Hibiscus sabdariffa (AEHS) on lipid profile parameters in hyperlipidaemia-induced Wistar rats. Different combinations of low (AEHS 250 mg/kg; simvastatin 10 mg/kg) and high doses (AEHS 500 mg/kg; simvastatin 20 mg/kg) were administered individually and concurrently daily for 2 and 4 weeks. Lipid profile parameters were assessed at these treatment periods. Subsequently, the effect of aqueous beverage of Hibiscus sabdariffa (ABHS) on the pharmacokinetics of single-dose 40 mg simvastatin was also evaluated in six healthy human volunteers using two-period randomized crossover design. Blood samples were collected at predetermined times for 24 hours. The plasma obtained was analysed for simvastatin using RP-HPLC/UV method. Aqueous extract of Hibiscus sabdariffa reduced total cholesterol (T c ) better than simvastatin (P = .031). Low-dose AEHS and low-dose simvastatin used concomitantly caused 38.3% and 57.4% reductions in T c and triglyceride levels, respectively, compared with low-dose simvastatin (P Hibiscus sabdariffa lowered T c better than simvastatin and enhanced the antihyperlipidaemic activity of the drug when co-administered at low doses in an animal model. However, aqueous beverage of Hibiscus sabdariffa caused a significant herb-drug interaction resulting in overall reduction in exposure to simvastatin in humans. Caution should

  15. Antihypoxic and antioxidant activity of Hibiscus esculentus seeds

    Directory of Open Access Journals (Sweden)

    Eslami, Bahman

    2010-03-01

    Full Text Available The antihypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Antihypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The antihypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC50 for DPPH radical-scavenging activity was 234 ± 8.9 μg ml-1. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml-1. The extracts showed weak Fe2+ chelating ability. IC50 were 150 ± 13 μg ml-1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts.La actividad antihipóxica y antioxidante de semillas de Hibiscus esculentus fue investigada empleando ocho ensayos in vitro. La actividad antihipóxica fue investigada en dos modelos, uno de caracter hemínico y otro circulatorio. Los efectos fueron pronunciados en ambos modelos de hipoxia. Los efectos antihipóxicos fueron dependientes de la dosis. Los resultados indican que los extractos tienen un efecto protector contra la letabilidad inducida por hipoxia en ratones. Los extractos mostraron actividad antioxidante en algunos modelos. El IC50 para la actividad captadora de radicales fue 234 ± 8.9 μg ml-1. Los extractos muestran una débil actividad captadora de óxido nítrico comprendida entre 0.1 y 1.6 mg ml-1. Los extractos muestran una débil capacidad quelatante de Fe2+. El IC

  16. Efek Hepatoprotektif Rosella (Hibiscus sabdariffa L. pada Tikus Model Hepatitis

    Directory of Open Access Journals (Sweden)

    Ike Rostikawati Husen

    2012-06-01

    Full Text Available Hepatitis is initiated by pollutant which caused oxidative stress. Rosella (Hibiscus sabdariffa L. contain flavonoid, an antioxidant which has been used traditionally in treating liver disease. The study aims to define the hepatoprotective effects of fresh rosella calyx ethanol extract (FRCEE on hepatitis model rats with liver malondialdehyde (MDA and serum glutamic piruvic transaminase (SGPT level and hepatocyte morphology damage compared to vitamin E’s effects. The laboratory experimental study has been conducted on August 2009 at Department Pharmacology and Therapy Faculty of Medicine Padjadjaran University/Dr. Hasan Sadikin Hospital in 25 male Wistar rats, divided into 5 groups. Group 1 was negative control, group 2 was positive control (carbon tetrachloride/CCl4-hepatitis model rats, group 3 and 4 were hepatitis model rats that subsequently given 125 mg/kgBW of FRCEE (group 3 and 2.7 IU of vitamin E (group 4 for 8 days; group 5 was given FRCEE. Observations were done 48 hours after CCL4 induction. The results were analyzed by analysis of varian (ANOVA and Tukey. The result showed that FRCEE inhibited increasing of liver MDA and SGPT level significantly different compared to positive control (MDA p=0.00, SGPT p=0.041 but not significantly different to group 4 (MDA p=1.00, SGPT p=0.192. Histological features showed macrovesicular on group 3, microvesicular vacuole on group 2; whereas vitamin E inhibited morphological damage. In conclusion, FRCEE has hepatoprotective effect on hepatitis model rats as antioxidant, but not superior compared to vitamin E.

  17. The effect of sour tea (Hibiscus sabdariffa) on essential hypertension.

    Science.gov (United States)

    Haji Faraji, M; Haji Tarkhani, A

    1999-06-01

    Considering the high prevalence of hypertension, its debilitating end organ damage, and the side effects of chemical drugs used for its treatment, we conducted this experimental study to evaluate the effect of sour tea (Hibiscus sabdariffa) on essential hypertension. For this purpose, 31 and 23 patients with moderate essential hypertension were randomly assigned to an experimental and control group, respectively. Patients with secondary hypertension or those consuming more than two drugs were excluded from the study. Systolic and diastolic blood pressures were measured before and 15 days after the intervention. In the experimental group, 45% of the patients were male and 55% were female, and the mean age was 52.6 +/- 7.9 years. In the control group, 30% of the patients were male, 70% were female, and the mean age of the patients was 51.5 +/- 10.1 years. Statistical findings showed an 11.2% lowering of the systolic blood pressure and a 10.7% decrease of diastolic pressure in the experimental group 12 days after beginning the treatment, as compared with the first day. The difference between the systolic blood pressures of the two groups was significant, as was the difference of the diastolic pressures of the two groups. Three days after stopping the treatment, systolic blood pressure was elevated by 7.9%, and diastolic pressure was elevated by 5.6% in the experimental and control groups. This difference between the two groups was also significant. This study proves the public belief and the results of in vitro studies concerning the effects of sour tea on lowering high blood pressure. More extensive studies on this subject are needed.

  18. Quercetin, kaempferol, myricetin, and fatty acid content among several Hibiscus sabdariffa accession calyces based on maturity in a greenhouse

    Science.gov (United States)

    Flavonols including quercetin, kaempferol, myricetin, and fatty acids in plants have many useful health attributes including antioxidants, cholesterol lowering, and cancer prevention. Six accessions of roselle, Hibiscus sabdariffa calyces were evaluated for quercetin, kaempferol, and myricetin conte...

  19. Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita

    Science.gov (United States)

    Pomegranate (Punica granatum) fruit and roselle (Hibiscus sabdariffa) leaves have been used in traditional medicine, including as anthelmintics. Methanolic extracts from these plants were investigated for activity against the southern root-knot nematode (RKN) Meloidogyne incognita. Dried, ground p...

  20. INDIKATOR TITRASI ASAM-BASA DARI EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L Indicator of Acid-Base Titration from the Extract of Hibiscus rosa sinensis L Flower

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2012-05-01

    Full Text Available Titration acid-base needs indicator  to show the change of color on interval of hydrogen exponent/degree of acid (pH. Indicator of synthetic which always be used have disadvantages like chemical pollution,  stock and expensive of pro- duction  cost. The research has been carried out to substitute the synthetic indicator with herbal indicator extracted from flower crown of Hibiscus rosa sinensis L extract. The herbal indicator was extracted from the flower crown Hibiscus rosa sinensis L using a mixture methanol-acetic acid. Then it was evaluated with phenolphthalein and methyl orange(E merck comparer to titration the acid-base, they are strong acid-strong base, weak base-strong acid and weak acid-strong base. The result of research show that herbal indicator  of flower crown Hibiscus rosa sinensis L to show theequivalent point in all titrations give peer result with the comparison. With the research result hoped that indicatortitration acid-base flower crown Hibiscus rosa sinensis L is able to as replace synthetic indicator  (metyl orange andphenolphtalein which always be used before. ABSTRAK Titrasi asam-basa memerlukan indikator untuk menunjukkan perubahan warna pada setiap interval derajad keasaman (pH. Indikator sintetis yang digunakan selama ini mempunyai beberapa kelemahan seperti polusi kimia, ketersediaan dan biaya produksi mahal. Upaya penelitian sudah dilakukan untuk menggantikan indikator sintetis dengan indikator dari ekstrak mahkota bunga sepatu. Indikator herbal tersebut dibuat dengan cara mengekstrak mahkota bunga Hibiscus rosa sinensis L dengan mengunakan pelarut metanol-asam asetat. Kemudian dievaluasi dengan indikator pembanding fenolftalein dan metil oranye (produksi E merck untuk titrasi asam-basa yaitu asam kuat-basa kuat, basa lemah-asam kuat dan asam lemah-basa kuat. Dari hasil penelitian diketahui bahwa indikator dari mahkota bunga sepatu untuk menunjukkan titik ekivalen dalam titrasi tersebut memberikan hasil yang

  1. Manfaat Ekstrak Bunga Rosella (Hibiscus Sabdariffa) Sebagai Obat Kumur Dalam Menghambat Pertumbuhan Plak Pada Mahasiswa FKG USU Angkatan 2012

    OpenAIRE

    G, Masbudi Harianto

    2013-01-01

    Masbudi Harianto G The use of the of Rosella flower extract (Hibiscus sabdariffa) as a mouthwash in slowing down plaque growth on FKG USU 2012 students. ix + 33 pages Nowadays, chemical plaque control is needed to overcome the limitation of mechanical plaque control. Herbal products were started to be developed for dentistry application especially as mouthwash. The purpose of this study was to determine the effectiveness of Rosella flower extract (Hibiscus sabdariffa) as mouthwash i...

  2. Mussel shell evaluation as bioindicator for heavy metals

    Energy Technology Data Exchange (ETDEWEB)

    Andrello, Avacir Casanova; Lopes, Fabio; Galvao, Tiago D. [Universidade Estadual de Londrina (UEL), PR (Brazil). Dept. de Fisica. Lab. de Fisica Nuclear Aplicada

    2009-07-01

    Full text: Recently, in Brazil, it has been appearing a new and unusual 'plague' in leisure and commercial fishing, caused by the parasitic larval phase of certain native bivalve mollusks of fresh water known as 'Naiades'. Such situation involves the presence of big bivalve of fresh water, mainly Anodontites trapesialis, in the tanks and dams of the fish creation, such bivalve mollusks belonging to the Ordem Unionoida and the Familia Mycetopodidae. The present work objectified to analyze the shells of such mollusks to verify the possibility of such mollusks as bioindicators of heavy metals in fresh water. The mollusks shells were collected in a commercial fishing at Londrina-PR, and analyzed qualitatively to determine the chemical composition and possible correlation with existent heavy metals in the aquatic environment. Studies of the literature have been showing that those mollusks are susceptible the existent chemical alterations in the aquatic environment due to anthropogenic action. Three different shells were analyzed, with the measures done on the external and internal side, using a portable Energy Dispersive X-Ray Fluorescence system (PXRF-LFNA-02). The measures were realized in the applied nuclear physics laboratory of State University of Londrina, and the PXRF-LFNA-02 is composed by a X-Ray tube (with Ag target and filter) with potency of 4W, and a detector Si-PIN model XR-100CR of Ampetc Inc. with resolution of 221eV for the line of 5.9 keV of the {sup 55}Fe (with a 25{mu}m Be window thickness and Ag collimator), Current 10 mA and High Voltage 28 kV. In the internal part of shells were identified the elements Ca, P, Fe, Mn and Sr and in the external part were identified Ca, P, Fe, Mn, Sr and Cu. The Ca ratio among the external and internal sides of the analyzed shells is around of 1, and it was expected because Ca is the main composed of mollusks shells. The ratio of P, Fe, Mn, and Sr for Ca stayed constant in all analyzed shells

  3. Comparison of four bioindication methods for assessing the degree of environmental lead and cadmium pollution

    Energy Technology Data Exchange (ETDEWEB)

    Dmuchowski, Wojciech, E-mail: w.dmuchowski@obpan.pl [Botanical Garden-Center for Conservation of Biological Diversity, Polish Academy of Science, Prawdziwka 2, 02-973 Warsaw (Poland); Warsaw University of Life Sciences-SGGW, Faculty of Agriculture and Biology, 159 Nowoursynowska 159, 02-776 Warsaw (Poland); Gozdowski, Dariusz, E-mail: dariusz_gozdowski@sggw.pl [Warsaw University of Life Sciences-SGGW, Faculty of Agriculture and Biology, 159 Nowoursynowska 159, 02-776 Warsaw (Poland); Baczewska, Aneta Helena [Botanical Garden-Center for Conservation of Biological Diversity, Polish Academy of Science, Prawdziwka 2, 02-973 Warsaw (Poland)

    2011-12-15

    Highlights: Black-Right-Pointing-Pointer In this study we examined level of contamination in extremely heavy polluted area. Black-Right-Pointing-Pointer We examined four various bioindication methods. Black-Right-Pointing-Pointer Results were presented in pollution maps. Black-Right-Pointing-Pointer We evaluated relationships between examined bioindication methods. - Abstract: The purpose of this study was to assess the application of several bioindication methods for the monitoring of environmental pollution from Pb and Cd. The study area centered on the town of Olkusz, Poland, which is one of the oldest centers for the metallurgical industry in Europe. The assessment of environmental pollution due to metals was performed using four frequently used bioindication methods: moss-bag (Sphagnum fallax), determination of metal accumulation in Pleurozium schreberi, silver birch foliage, and Scots pine needles. The region of Olkusz, and especially the area surrounding the mining and metallurgical Boleslaw complex, was extremely contaminated with Pb and Cd. The results of the investigations are presented as contamination deposition maps. Despite the application of various methods and the resulting diversity of the specific exposure periods for different biomonitors, the spatial distribution of contamination shown on the maps was similar, as confirmed by the statistical analysis of the results.

  4. Gastrointestinal parasites of fish as bio-indicators of the ecology of ...

    African Journals Online (AJOL)

    Fish parasites are of particular interest in relation not only to fish health but also in the understanding of ecological problems. A study of the gastrointestinal parasites of fish as bio-indicators of heavy metal pollution in Chanchaga River was carried out from May-August, 2014. A total of 100 specimens were sampled which ...

  5. Bioindicator plants as monitoring tools for urban and industrial pollutant sources - case studies from Austria

    Energy Technology Data Exchange (ETDEWEB)

    Soja, G.; Schafler, P.; Gerzabek, M. [Austrian Research Center Seibersdorf (Austria). Dept. of Environmental Research

    2002-07-01

    This paper presents two examples for the application of bioindicator techniques in environmental monitoring projects. The results presented here refer to the quantitation of the effects of environmental parameters on the behaviour of bioindicator plants. a.) Environmental monitoring of an industrial pollutant emitter in a rural environment. For monitoring the environmental impact of a coal-fired power plant, an extensive multi-annual monitoring programme had been established four years prior to the start of operation of the power plant. The programme included analyses of soils and field crops and the physiological responses of crop plants in non-filtered and carbon-filtered open-top chambers (OTC). b.) Monitoring of ozone effects on bioindicator plants in a large city and its rural environs. In a two-year multi-location study, the region of Vienna was analysed along a profile from the west to the southeast of the city for visual ozone injury of bioindicator plants. Meteorological conditions and air pollutant concentrations were recorded in detail at 9 out of 25 indicator stations. The resulting dataset was processed with multivariate techniques. (orig.)

  6. Game animals and small terrestrial mammals - Suitable bioindicators for the pollution assessment in agrarian ecosystems

    Czech Academy of Sciences Publication Activity Database

    Vávrová, M.; Zlámalová Gargošová, H.; Šucman, E.; Večerek, V.; Kořínek, P.; Zukal, Jan; Zejda, Jan; Sebestiánová, N.; Kubištová, I.

    2003-01-01

    Roč. 12, č. 2 (2003), s. 165-172 ISSN 1018-4619 R&D Projects: GA AV ČR KSK6005114 Keywords : bioindicators * agrarian ecosystems * pollution Subject RIV: EH - Ecology, Behaviour Impact factor: 0.325, year: 2003 http://www.psp-parlar.de/details_artikel.asp?tabelle=FEBArtikel&artikel_id=234&jahr=2003

  7. Comparison of four bioindication methods for assessing the degree of environmental lead and cadmium pollution

    International Nuclear Information System (INIS)

    Dmuchowski, Wojciech; Gozdowski, Dariusz; Baczewska, Aneta Helena

    2011-01-01

    Highlights: ► In this study we examined level of contamination in extremely heavy polluted area. ► We examined four various bioindication methods. ► Results were presented in pollution maps. ► We evaluated relationships between examined bioindication methods. - Abstract: The purpose of this study was to assess the application of several bioindication methods for the monitoring of environmental pollution from Pb and Cd. The study area centered on the town of Olkusz, Poland, which is one of the oldest centers for the metallurgical industry in Europe. The assessment of environmental pollution due to metals was performed using four frequently used bioindication methods: moss-bag (Sphagnum fallax), determination of metal accumulation in Pleurozium schreberi, silver birch foliage, and Scots pine needles. The region of Olkusz, and especially the area surrounding the mining and metallurgical Bolesław complex, was extremely contaminated with Pb and Cd. The results of the investigations are presented as contamination deposition maps. Despite the application of various methods and the resulting diversity of the specific exposure periods for different biomonitors, the spatial distribution of contamination shown on the maps was similar, as confirmed by the statistical analysis of the results.

  8. Study of ranking of bio-indices using benthic macroinvertebrates for Lower Dongnai River System, Vietnam.

    Science.gov (United States)

    Nga, Le Phi; Quoi, Le Phat; Duc, Pham Anh

    2012-04-01

    It has been demonstrated by research that the most successful assessment methods have been based on the benthic macroinvertebrate communities. A lot of bio-indices have been applied to evaluate the water quality widely. However, most of them have got the rankings for the water quality assessment. In this study, based on the monitoring results of the benthic macroinvertebrate and the environmental parameters in the Lower Dongnai River System during three-year periods (2007 - 2009), the linear correlations among the most popular bio-indices and each environmental parameter were considered. These environmental variables having the most closed correlation with the biological indices were DO (dissolved oxygen), WQI (water quality index) and total nitrogen. From the analysis, the ranking of bio-indices using benthic macroinvertebrate for the Lower Dongnai River System were established. The findings proved that the ranking of bio-indices for water quality assessment can be used to evaluate the water quality for the Lower Dongnai River System.

  9. Chemical composition of some Roselle (Hibiscus sabdariffa) genotypes

    International Nuclear Information System (INIS)

    Alshoosh, Wafa Gamal Abdalla

    1997-10-01

    Six lines of of Hibiscus sabdariffa were used in this study. The proximate analysis gave moisture 6.19%, 12.07% and 8.42%, ash 10.6%, 7.98% and 12.42%, protein 9.76%, 5.53% and 6.13%, oil 0.99%, 0.95% and 0.90%, fibre 10.74%, 11.73% and 12.17%, carbohydrate 61.64%, 61.76% and 60.43% for seasons 93/94, 94/95 and 95/96 respectively. Physicochemical investigation of karkade calices gave the following results: reducing sugar 1.29%. 1.28% and 1.27%, non reducing sugar 0.87, 0.77 and 0.86, PH 3.1, 2.71 and 3.001, colour intensity 0.77, 0.72 and 0.51, insoluble solids 37.74%, 36.36% and 37.33% for seasons 93/94, 94/95 and 95/96 respectively. The value of vitamin C and acidity (organic acids) in season 93/94 was 92.06 mg/100g and 19.58% respectively. Organic acids as hibiscic acid, citric acid, vitamin C and xalic acid was 25.51%, 14.15%, 92.69 mg/100g and 0.55% and 26.18%, 14.14%, 97.26 mg/100g and 0.56% for season 94/95 and 95/96 respectively. Mineral contents for karkade calices were measured by using atomic absorption spectroscopy, the results for season 94/95 were 0.56%, 0.24%, 0.08%, 0.23%, 0.95% and 0.19% for Ca, Mg, Fe, Na, K and P respectively, while mineral content for season 95/96, 1.9%, 0.31%, 0.07%, 0.31%, 0.48%, 0.23%. 0.05%, 0.22%, 0.06%, 0.006%, 0.02%, and 0.03% for Ca, Mg, Fe, Na, K, P, Zn, M, Cu, Pb, Co and Cr respectively. UV-visible spectrum showed the presence of the following pigments: dephinidin-3-sambubioside and cyanidin-3-sambuioside. Total anthocyanin of karkade calices were found to be 0.89 to 1.4% and 0.99 to 1.6% for season 94/95 and 95/96 respectively. Acid hydrolysis of the pigments showed the presence of xylose and glucose in addition to the anthocyanidins delphinidin ad cyanidin.(Author)

  10. Diuretic effect of compounds from Hibiscus sabdariffa by modulation of the aldosterone activity.

    Science.gov (United States)

    Jiménez-Ferrer, Enrique; Alarcón-Alonso, Javier; Aguilar-Rojas, Arturo; Zamilpa, Alejandro; Jiménez-Ferrer C, Itzia; Tortoriello, Jaime; Herrera-Ruiz, Maribel

    2012-12-01

    Recent studies of Hibiscus sabdariffa Linn. have demonstrated that it presents diuretic, natriuretic, and potassium sparing effects. However, the mechanism that induces these effects has not yet been elucidated. The aim of this study was to explore the possible mechanism of action for the diuretic effect of Hibiscus sabdariffa extract and its fractions.The aqueous extract from this plant and the fractions obtained with solvents of different polarities were administered to adrenalectomized rats, and the diuretic effect was measured in the presence of deoxycorticosterone acetate (aldosterone analog).The effect on renal filtration was also evaluated in an in situ kidney model, and finally, the effect of diuretic active extracts on gene expression of the alpha subunit from the transporter (αENaC) of renal epithelial cell was quantified. The subsequent results were obtained: The aqueous extract of Hibiscus sabdariffa presented the following chemical composition, 32.4 mg/g delphinidin-3-O-sambubioside, 11.5 mg/g cyanidin-3-O-sambubioside, 11.5 mg/g quercetin, and chlorogenic acid 2.7 mg/g. The concentration of anthocyanins was diminished until disappearance due to decrease of the polarity of the solvents used in the extraction process, in contrast to the flavonoids and chlorogenic acid, which had their concentration increased. The diuretic effect caused by adrenalectomy in rats was reversed by deoxycorticosterone acetate activity. However, the effect of deoxycorticosterone acetate was antagonized by spironolactone, the aqueous extract of Hibiscus sabdariffa, and the acetonitrile : methanol 5 : 5 mixture extract, administered orally. A similar effect was observed on renal filtration obtained from the isolated kidney model.When the gene expression levels of αENaC was measured in adrenalectomized rats, it was observed that spironolactone, the aqueous extract of Hibiscus sabdariffa, the acetonitrile : methanol 5 : 5 mixture, as well as the

  11. Lipophilization and MS characterization of the main anthocyanins purified from hibiscus flowers.

    Science.gov (United States)

    Grajeda-Iglesias, Claudia; Salas, Erika; Barouh, Nathalie; Baréa, Bruno; Figueroa-Espinoza, Maria Cruz

    2017-09-01

    Hibiscus sabdariffa flowers represent an interesting source of anthocyanins, one of the most important plant pigments, which are responsible of the intense red color of the calyces, and have potential as natural colorants for food applications. Nevertheless, anthocyanins are highly hydrosoluble and unstable compounds. On this basis, the aim of this work was to increase the lipophilicity of the hibiscus anthocyanins by lipophilization, in order to obtain amphiphilic colorants, which could be easily incorporated in lipid-rich food matrices. Octanoyl derivatives of delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside were chemically obtained for the first time, and characterized by means of HPLC-ESI-MS data. Copyright © 2017. Published by Elsevier Ltd.

  12. Phytochemical analysis of Hibiscus caesius using high performance liquid chromatography coupled with mass spectrometry.

    Science.gov (United States)

    Ain, Quratul; Naveed, Muhammad Na; Mumtaz, Abdul Samad; Farman, Muhammad; Ahmed, Iftikhar; Khalid, Nauman

    2015-09-01

    Various species in genus Hibiscus are traditionally known for their therapeutic attributes. The present study focused on the phytochemical analysis of a rather unexplored species Hibiscus caesius (H. caesius), using high-pressure liquid chromatography coupled with mass spectrometry (HPLC-MS). The analysis revealed five major compounds in the aqueous extract, viz. vanillic acid, protocatechoic acid, quercetin, quercetin glucoside and apigenin, being reported for the first time in H. caesius. Literature suggests that these compounds have important pharmacological traits such as anti-cancer, anti-inflammatory, anti-bacterial and hepatoprotective etc. however, this requires further pharmacological investigations at in vitro and in vivo scale. The above study concluded the medicinal potential of H. caesius.

  13. Destruction of the Phoenix/Hibiscus and Barringtonia racemosa Communities at Richards Bay, Natal, South Africa

    Directory of Open Access Journals (Sweden)

    P. J. Weisser

    1982-10-01

    Full Text Available The destruction of the Phoenix!Hibiscus and Barringtonia racemosa Communities described by Venter in 1972 on the southern shores of Richards Bay is reported. The cause was the artificial openingof a new mouth about 5,5 km south of the original mouth, which increased tidal range and salinity. These swamp communities occupied a narrow band about 6 ha in area behind the Bruguiera gymnorrhiza Community. An estimated 95 % of the communities was affected and only on the landward border were some isolated remnants of species such as Acrostichum aureum, Hibiscus tiliaceus and Phoenix reclinata detected .Young stands of  Phragmites australis, seedlings of  Bruguiera gymnorrhiza and Avicennia marina and epipelic algae are recoIonizing the affected area.

  14. The South American species of Hibiscus sect. Furcaria DC. (Malvaceae-Hibisceae

    Directory of Open Access Journals (Sweden)

    Antonio Krapovickas

    2004-01-01

    Full Text Available The Hibiscus section Furcaria from South America is revised. Ten new species from Brasil are described: H. Andersonii, H. capitalensis, H. chapadensis, H. Gregoryi, H. Hochreutineri, H. itirapinensis, H. matogrossensis, H. Nanuzae, H. Saddii, H. Windischii, and a new one from Perú: H. Chancoae. Two new names are proposed: H. Hilarianus from Brasil and H. amambayensis from Paraguay. A key is provided to distinguish the 40 species of section Furcaria known from South America

  15. Evaluation of various physico-chemical properties of Hibiscus sabdariffa and L. casei incorporated probiotic yoghurt.

    Science.gov (United States)

    Rasdhari, M; Parekh, T; Dave, N; Patel, V; Subhash, R

    2008-09-01

    The present investigation was carried out to examine the effect of Hibiscus sabdariffa Calyx extract on the physico-chemical properties, sensory attributes, texture and microbial analysis of L. casei incorporated in probiotic yoghurt after manufacture and during storage. Incorporation of Hibiscus sabdariffa Calyx extract into the probiotic yoghurt resulted into decrease in coagulation time by 25 min. The pH ranged from 4.39 to 4.59, TA 0.81 to 1.14%, moisture 3.05 to 3.37 g%, syneresis 18.85 to 24.90 mL/50 g of sample, % inhibition 12.32 to 59.43, TS 21.27 to 24.90 g% and beta-galactosidase activity 1.041 to 3.277. The protein content ranged between 4.11 and 4.14 g% while the fat content ranged between 3.43 and 3.49 g%. No major changes in sensory evaluation were observed on the day of manufacture and during storage for 7 days. Sabdariffa added yoghurt showed a higher score in almost all sensory attributes. Microbial analysis showed a total plate count ranging from 1.8 x 10(4) to 1.85 x 10(7) cfu mL(-1). Yeast and mold counts were negligible in the Sabdariffa yoghurts. Thus the study concludes that incorporation of Hibiscus sabdariffa extract in yoghurt improved the total antioxidant property, organoleptic qualities and decreased the exudation of whey proteins (Syneresis). Thus, Hibiscus sabdariffa Calyces has beneficial influence on the quality of L. casei incorporated probiotic yoghurt.

  16. Synthesis, Characterization and Evaluation of the Transformations in Hibiscus sabdariffa-graft-poly(butyl acrylate

    Directory of Open Access Journals (Sweden)

    B. S. Kaith

    2008-01-01

    Full Text Available Different reaction parameters for the graft copolymerization of butyl acrylate onto Hibiscus sabdariffa fiber were optimized. Graft copolymers thus obtained were subjected to characterization using XRD, TGA, DTA, SEM and FTIR techniques and were evaluated for physio-chemical changes in the behavior. The percentage crystallinity and crystallinity index were found to decrease with increase in grafting while there was reduction in moisture absorption and increase in chemical, thermal resistance of the graft copolymers.

  17. Combination of aerobic exercise and Hibiscus sabdariffa Linn. increased nitric oxide in rats

    OpenAIRE

    Donna Adriani Kusumadewi Muhammad; Minarma Siagian; Dewi Irawati SS

    2017-01-01

    Background Hypertension and myocardial infarction account for the high rate of mortality globally. Hibiscus sabdariffa (HS) Linn. is rich in antioxidants and previous studies have demonstrated its anti-hypertensive effects. Several studies show that regular physical activity is an important component to reduce cardiovascular mortality. The objective of this study was to evaluate the effects of a combination of aerobic exercise and HS extract on nitric oxide (NO) and endothelin-1 (ET-1) i...

  18. ROSELLE FLOWER (HIBISCUS SABDARIFFA) IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

    OpenAIRE

    Nikmah Jalilah Ritonga; Onny Setiani; Umaroh; Kamilah Budhi R; Faisal Amri

    2017-01-01

    Background: Roselle flower (Hibiscus sabdariffa) has been used as a food and herbal drinks, in hot and cold beverages, as a herbal medicine. Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period. Methods: This was a Quasi experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited by consecutive sampling with 15 samples in the intervention an...

  19. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    OpenAIRE

    Kaleemullah, M.; Jiyauddin, K.; Thiban, E.; Rasha, S.; Al-Dhalli, S.; Budiasih, S.; Gamal, O.E.; Fadli, A.; Eddy, Y.

    2016-01-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop susta...

  20. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

    OpenAIRE

    Gheller, Ana Carla Guidini Valentini; Kerkhoff, Jacqueline; Vieira Júnior, Gerardo Magela; Campos, Kleber Eduardo de; Sugui, Marina Mariko

    2017-01-01

    Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25?mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality o...

  1. The flower of Hibiscus trionum is both visibly and measurably iridescent.

    Science.gov (United States)

    Vignolini, Silvia; Moyroud, Edwige; Hingant, Thomas; Banks, Hannah; Rudall, Paula J; Steiner, Ullrich; Glover, Beverley J

    2015-01-01

    Living organisms can use minute structures to manipulate the reflection of light and display colours based on interference. There has been debate in recent literature over whether the diffractive optical effects produced by epoxy replicas of petals with folded cuticles persist and induce iridescence in the original flowers when the effects of petal pigment and illumination are taken into account. We explored the optical properties of the petal of Hibiscus trionum by macro-imaging, scanning and transmission electron microscopy, and visible and ultraviolet (UV) angle-resolved spectroscopy of the petal. The flower of Hibiscus trionum is visibly iridescent, and the iridescence can be captured photographically. The iridescence derives from a diffraction grating generated by folds of the cuticle. The iridescence of the petal can be quantitatively characterized by spectrometric measurements with several square-millimetres of sample area illuminated. The flower of Hibiscus trionum has the potential to interact with its pollinators (honeybees, other bees, butterflies and flies) through iridescent signals produced by its cuticular diffraction grating. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  2. Anti-inflammatory activity and molecular mechanism of delphinidin 3-sambubioside, a Hibiscus anthocyanin.

    Science.gov (United States)

    Sogo, Takayuki; Terahara, Norihiko; Hisanaga, Ayami; Kumamoto, Takuma; Yamashiro, Takaaki; Wu, Shusong; Sakao, Kozue; Hou, De-Xing

    2015-01-01

    Delphinidin 3-sambubioside (Dp3-Sam), a Hibiscus anthocyanin, was isolated from the dried calices of Hibiscus sabdariffa L, which has been used for folk beverages and herbal medicine although the molecular mechanisms are poorly defined. Based on the properties of Dp3-Sam and the information of inflammatory processes, we investigated the anti-inflammatory activity and molecular mechanisms in both cell and animal models in the present study. In the cell model, Dp3-Sam and Delphinidin (Dp) reduced the levels of inflammatory mediators including iNOS, NO, IL-6, MCP-1, and TNF-α induced by LPS. Cellular signaling analysis revealed that Dp3-Sam and Dp downregulated NF-κB pathway and MEK1/2-ERK1/2 signaling. In animal model, Dp3-Sam and Dp reduced the production of IL-6, MCP-1 and TNF-α and attenuated mouse paw edema induced by LPS. Our in vitro and in vivo data demonstrated that Hibiscus Dp3-Sam possessed potential anti-inflammatory properties. © 2015 International Union of Biochemistry and Molecular Biology.

  3. The Effect of Hibiscus Sabdariffa on Lipid Profile, Creatinine, and Serum Electrolytes: A Randomized Clinical Trial

    Science.gov (United States)

    Mohagheghi, Abbas; Maghsoud, Shirin; Khashayar, Patricia; Ghazi-Khansari, Mohammad

    2011-01-01

    Background. Hibiscus Sabdariffa L. (HS), a member of malvaceae family, is a medicinal plant with a worldwide fame. Its effect on reducing serum lipids is mentioned in several studies. The purpose of this study was to assess the efficacy of this plant in reducing the serum's lipids in hypertensive patients. Materials and Methods. Ninety hypertensive patients were randomly assigned to receive Hibiscus Sabdariffa (HS) tea or black tea for 15 days. The patients were asked to drink the tea within 20 minutes following its preparation. This process had to be repeated two times, daily. Patient's FBS and lipid profile were collected at the first visit day (day 0) and on the day 30. Results. There was no significant differences between pre and post experiment values within the two groups. An upward trend in total cholesterol, HDL, and LDL cholesterol was evident in both groups. The increase in total and HDL cholesterol in both groups relative to their initial values were significant. Conclusion. Hibiscus Sabdariffa is probably a safe medicinal plant. No significant harmful changes in cholesterol, triglyceride, BUN, serum creatinine, Na and K levels were observed within 15 days after the discontinuation of the medication. PMID:21991538

  4. Neuroprotective effect of the methanolic extract of Hibiscus asper leaves in 6-hydroxydopamine-lesioned rat model of Parkinson's disease.

    Science.gov (United States)

    Hritcu, Lucian; Foyet, Harquin Simplice; Stefan, Marius; Mihasan, Marius; Asongalem, Acha Emmanuel; Kamtchouing, Pierre

    2011-09-01

    While the Hibiscus asper Hook.f. (Malvaceae) is a traditional herb largely used in tropical region of the Africa as vegetable, potent sedative, tonic and restorative, anti-inflammatory and antidepressive drug, there is very little scientific data concerning the efficacy of this. The antioxidant and antiapoptotic activities of the methanolic extract of Hibiscus asper leaves (50 and 100 mg/kg) were assessed using superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) specific activities, total glutathione (GSH) content, malondialdehyde (MDA) level (lipid peroxidation) and DNA fragmentation assays in male Wistar rats subjected to unilateral 6-hydroxydopamine (6-OHDA)-lesion. In 6-OHDA-lesioned rats, methanolic extract of Hibiscus asper leaves showed potent antioxidant and antiapoptotic activities. Chronic administration of the methanolic extract (50 and 100 mg/kg, i.p., daily, for 7 days) significantly increased antioxidant enzyme activities (SOD, GPX and CAT), total GSH content and reduced lipid peroxidation (MDA level) in rat temporal lobe homogenates, suggesting antioxidant activity. Also, DNA cleavage patterns were absent in the 6-OHDA-lesioned rats treated with methanolic extract of Hibiscus asper leaves, suggesting antiapoptotic activity. Taken together, our results suggest that the methanolic extract of Hibiscus asper leaves possesses neuroprotective activity against 6-OHDA-induced toxicity through antioxidant and antiapoptotic activities in Parkinson's disease model. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  5. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat

    Science.gov (United States)

    Gauthaman, Karunakaran K; Saleem, Mohamed TS; Thanislas, Peter T; Prabhu, Vinoth V; Krishnamoorthy, Karthikeyan K; Devaraj, Niranjali S; Somasundaram, Jayaprakash S

    2006-01-01

    Background The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects. Methods The medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose) have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms) in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis. Results There was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS) [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress) occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury. Conclusion It may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury. PMID:16987414

  6. Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa.

    Science.gov (United States)

    Ojeda, Deyanira; Jiménez-Ferrer, Enrique; Zamilpa, Alejandro; Herrera-Arellano, Armando; Tortoriello, Jaime; Alvarez, Laura

    2010-01-08

    The beverages of Hibiscus sabdariffa calyces are widely used in Mexico as diuretic, for treating gastrointestinal disorders, liver diseases, fever, hypercholesterolemia and hypertension. Different works have demonstrated that Hibiscus sabdariffa extracts reduce blood pressure in humans, and recently, we demonstrated that this effect is due to angiotensin converting enzyme (ACE) inhibitor activity. The aim of the current study was to isolate and characterizer the constituents responsible of the ACE activity of the aqueous extract of Hibiscus sabdariffa. Bioassay-guided fractionation of the aqueous extract of dried calyces of Hibiscus sabdariffa using preparative reversed-phase HPLC, and the in vitro ACE Inhibition assay, as biological monitor model, were used for the isolation. The isolated compounds were characterized by spectroscopic methods. The anthocyanins delphinidin-3-O-sambubioside (1) and cyanidin-3-O-sambubioside (2) were isolated by bioassay-guided purification. These compounds showed IC(50) values (84.5 and 68.4 microg/mL, respectively), which are similar to those obtained by related flavonoid glycosides. Kinetic determinations suggested that these compounds inhibit the enzyme activity by competing with the substrate for the active site. The competitive ACE inhibitor activity of the anthocyanins 1 and 2 is reported for the first time. This activity is in good agreement with the folk medicinal use of Hibiscus sabdariffa calyces as antihypertensive. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.

  7. Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L.) as a photosensitizer on nanostructured-TiO2 dye sensitized solar cells

    Science.gov (United States)

    Ramelan, A. H.; Wahyuningsih, S.; Rosyida, N. A.; Supriyanto, E.; Saputro, S.; Hanif, Q. A.; Rinawati, L.

    2016-02-01

    Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L) as a photosensitizer in nanostructured-TiO2 dye sensitized solar cells has been fabricated. Ultravisible visible absorption spectra of anthocyanin show an ability absorbing light in the visible region. While the nanostructed-TiO2 powder in this research was prepared by sol-gel method following annealled at a temperature of 600°C. Subsequently, the TiO2 nanostructures were characterized by XRD, XRF, and SEM. The difractogram X-ray results shown that TiO2 was built from f anatase and rutile phase. Element analysis of synthesized TiO2 by X-ray Fluorecence (XRF) shown the TiO2 content of 98,67 wt%. TiO2 layer prepared at different thickness showed the average size of cavity about 0.83 µm. These several thickness of solar cells were fabricated and were immersed into anthocyanin for 24 hours to gain sensitized TiO2 photoanode for Dye sensitised solar cells (DSSCs). These DSSCS performance were measured using I-V Keithley 2602A. The results exhibited that the sample with a TiO2 layer thickness of 4.75 ± 0.8 µm has the highest efficiency.

  8. Evaluation of diffussivity and acceptable mixing ratio for production of hibiscus-moringa tea bags

    Directory of Open Access Journals (Sweden)

    J. A. Famurewa

    2017-08-01

    Full Text Available This study was conducted to evaluate the diffusivity and mixing ratio of hibiscus/moringa in tea bags.Hibiscus and moringa leaves were mixed at different ratios and labeled as samples A (70:30, B (60:40, C (50:50, D (40:60 and E (20:80. Lipton tea was used as control sample. Standard methods were used to determine the proximate compositions, pH, total soluble solids, and diffusion rate. Twenty (20 semi trained panelists conducted the sensory evaluation tests. The results of the proximate analysis show that the protein content ranged from 20.13 to 32.73 g/100g; increasing with increase in moringa content, the moisture content ranged from 3.05to 3.97 g/100g, the crude fibre content ranged from 10.13 to 11.93 g/100g. The ash content ranged from 9.70 to 11.14 g/100g, the fat content ranged from 9.67 to 10.24 g/100g. The result of the brix content ranged from 1.80 to 3.00 oBrix. The pH of the leave blends ranged from 3.30 to 4.06 with sample D having the highest pH and sample B having the lowest.The result of the diffusion rate ranged from 0.39 to 0.60 g/min; sample with 60% moringa and 40% hibiscus composition having the highest. The sensory evaluation shows that sample E (70% moringa and 30% hibiscus was most preferred in terms of appearance, taste and general acceptability. The results of the study show that sample C (50% moringa and 50% hibiscus had no significant difference in colour and general acceptability to control and sample E which was most accepted among all the samples, sample C has a significant different value of protein to sample E Sample C was therefore recommended for consumption for the amelioration of the incidence of protein malnutrition in developing countries.

  9. Evaluation of the Sagittaria montevidensis Cham. & Schltdl. as a bioindicator and phytoextractor of toxic metals

    Directory of Open Access Journals (Sweden)

    Edila Maria Kickhöfel Ferrer

    2017-12-01

    Full Text Available This study evaluated the bioindication and phytoremediation capacity of the aquatic macrophyte Sagittaria montevidensis by using it to assess the bioconcentration and translocation of heavy metals. A simple sampling was conducted at four sites in the region of Pelotas, southern Brazil, where plants, water and sediments were collected. The plants were subjected to nitric-perchloric acid digestion and the sediments underwent pseudo-total acid digestion. The determination of the elements Cr, Cu, Pb, Ni and Zn in the extracts was performed by flame atomic absorption spectrometry. Physicochemical tests were also carried out on water samples, including pH, electrical conductivity, chlorides, alkalinity, hardness and organic matter, and in sediment samples, moisture and organic matter content. The macrophyte study indicated a Bioconcentration Factor (BCF and a Translocation Factor (TF, especially for Ni. The results showed that the aquatic macrophyte species S. montevidensis has a phytoextraction capacity, mainly for Ni, and potentially for bioindication of Cu, Ni and Zn.

  10. Spatial and temporal patterns of bioindicator mercury in pennsylvania oak forest.

    Science.gov (United States)

    McClenahen, James R; Hutnik, Russell J; Davis, Donald D

    2013-01-01

    We monitored spatial and temporal patterns of total Hg in forest bioindicators to assess possible local, regional, and global changes in atmospheric Hg deposition. Total Hg concentrations were monitored in leaves and fresh litterfall of northern red oak ( L.), on an epiphytic moss ( Hedw.) on northern red oak stems, and in surface soil organic matter (O and O horizons) in Pennsylvania oak-dominated forests. Variously configured plots were used to monitor Hg deposition near local coal-fired generating stations and an industrial city and along an extended regional transect. Linearly decreasing temporal trends in Hg concentrations occurred in leaves, litterfall, moss, and soil O and O. Mean annual Hg concentrations were often greater near local emissions sources compared with remote areas, especially in the initial monitoring period. Decreasing time trends for different impact areas tended to converge due to greater rates of Hg decrease where initial bioindicator Hg levels were higher. Fresh litter and soil O showed the greatest overall potential as Hg bioindicators. We conclude that Hg deposition has been significantly decreasing over time throughout the study area as a result of locally and regionally declining Hg emissions. Reductions in Hg emissions are likely a co-benefit of the 1990 Clean Air Act regulations and changing industrial activities. Recent leveling of several bioindicator Hg time trends may foretell a shift in Hg depositional patterns. Mercury monitoring studies such as this fulfill a need for documenting local and regional effects of emissions reduction. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  11. Trees as bioindicator of heavy metal pollution in three European cities

    International Nuclear Information System (INIS)

    Sawidis, T.; Breuste, J.; Mitrovic, M.; Pavlovic, P.; Tsigaridas, K.

    2011-01-01

    Concentrations of four heavy metals were determined in tree leaves and bark collected from polluted and non-polluted areas of three European cities (Salzburg, Belgrade and Thessaloniki) for a comparative study. Platanus orientalis L. and Pinus nigra Arn., widespread in urban northern and southern Europe, were tested for their suitability for air quality biomonitoring. Leaves and barks were collected uniformly of an initial quantity of about 30 g of each sample. Analysis was accomplished by electrothermal atomic absorption spectrometry after total digestion. Site-dependent variations were found with the highest concentration level measured in Belgrade, followed by Thessaloniki and Salzburg. A higher accumulation of heavy metals was found in bark compared to leaves. Pine tree bark, accumulating higher concentrations of trace metals compared to plane tree bark, shows a higher efficiency as bioindicator for urban pollution. Both indicator species are suitable for comparative studies on bioindication of urban air pollution. - Highlights: → Oriental plane and Austrian pine are suitable for comparative urban air quality biomonitoring of heavy metal pollution. → Pine tree is excellently suitable as urban bioindicator as it accumulates high concentrations of trace metals. → The highest heavy metal pollution was found in Belgrade followed by Thessaloniki and Salzburg. - Oriental plane (Platanus orientalis L.) and Austrian pine (Pinus nigra Arn.), widespread in urban northern and southern Europe, are suitable for comparative biomonitoring of urban air pollution.

  12. Bioindicators of Air Quality Species | Bioindicadores de espécies de qualidade do ar

    Directory of Open Access Journals (Sweden)

    Cleuma Christir Silva Almeida

    2017-06-01

    Full Text Available The research aimed to identify biomarkers (trees and lichens used in studies related to air pollution published in scientific articles in the years 2013 and 2016 worldwide. It performed a literature review based on scientific article published between the years 2013-2016, seeking to indicate the main bio-indicators species of lichens and individual trees, as well as the local indication that favors the highest concentration of atmospheric pollutant. The most studied species of lichens were Flavoparmelia caperata (L. Evernia prunastri (L. Ach., Parmelia sulcata Taylor, L. Pseudevernia furfuracea Zopf, all cited in at least two studies. By contrast, the forest species have been little studied preventing the indication of the species that are considered bioindicators, requiring further study. The concentrations of PAHs and heavy metals in the atmosphere influence the diversity and distribution of species of both lichens, as the tree species, which may be related to sensitivity that one may have in relation to the other. Such concentrations are influenced by climate change, humidity, wind direction, altitude, different uses and occupation, distance and type of pollution. The bioindicators raised proved to be quite relevant, however it lacks a methodological standardization, hindering greater intraspecific and interspecific comparisons.

  13. The use of the brown macroalgae, Sargassum flavicans, as a potential bioindicator of industrial nutrient enrichment.

    Science.gov (United States)

    Alquezar, Ralph; Glendenning, Lionel; Costanzo, Simon

    2013-12-15

    Nutrient bioindicators are increasingly being recognised as a diagnostic tool for nutrient enrichment of estuarine and marine ecosystems. Few studies, however, have focused on field translocation of bioindicator organisms to detect nutrient discharge from industrial waste. The brown macroalgae, Sargassum flavicans, was investigated as a potential bioindicator of nutrient-enriched industrial effluent originating from a nickel refinery in tropical north-eastern Australia. S. flavicans was translocated to a number of nutrient enriched creek and oceanic sites over two seasons and assessed for changes in stable isotope ratios of (15)N and (13)C within the plant tissue in comparison to reference sites. Nutrient uptake in macroalgae, translocated to the nutrient enriched sites adjacent to the refinery, increased 3-4-fold in δ(15)N, compared to reference sites. Using δ(15)N of translocated S. flavicans proved to be a successful method for monitoring time-integrated uptake of nitrogen, given the current lack of passive sampler technology for nutrient monitoring. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Application of indicator kriging to the complementary use of bioindicators at three trophic levels

    Energy Technology Data Exchange (ETDEWEB)

    Figueira, Rui, E-mail: rui.figueira@iict.p [Jardim Botanico Tropical, Instituto de Investigacao Cientifica Tropical, Trav. Conde da Ribeira, 9, 1300-142 Lisboa (Portugal); CERENA, Instituto Superior Tecnico, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); Tavares, Paula C. [CVRM-Geo-Systems Centre, Instituto Superior Tecnico, Av. Rovisco Pais, 1049-001 Lisboa (Portugal); Palma, Luis [CCMAR, Universidade do Algarve, FCMA, Campus de Gambelas, 8005-139 Faro (Portugal); Beja, Pedro [ERENA, Ordenamento e Gestao de Recursos Naturais, Rua Robalo Gouveia, 1-1A, 1900-392 Lisboa (Portugal); CIBIO, Centro de Investigacao em Biodiversidade e Recursos Geneticos, Campus Agrario de Vairao, Universidade do Porto, Vairao (Portugal); Sergio, Cecilia [Jardim Botanico, Museu Nacional de Historia Natural, Universidade de Lisboa, R. Escola Politecnica, 58, 1250-102 Lisboa (Portugal); CBA, Faculdade de Ciencias da Universidade de Lisboa, Campo Grande, Edificio C2, 1749-016 Lisboa (Portugal)

    2009-10-15

    The use of biological indicators is widespread in environmental monitoring, although it has long been recognised that each bioindicator is generally associated with a range of potential limitations and shortcomings. To circumvent this problem, this study adopted the complementary use of bioindicators representing different trophic levels and providing different type of information, in an innovative approach to integrate knowledge and to estimate the overall health state of ecosystems. The approach is illustrated using mercury contamination in primary producers (mosses), primary consumers (domestic pigeons and red-legged partridges) and top predators (Bonelli's eagles) in southern Portugal. Indicator kriging geostatistics was used to identify the areas where mercury concentration was higher than the median for each species, and to produce an index that combines mercury contamination across trophic levels. Spatial patterns of mercury contamination were consistent across species. The combined index provided a new level of information useful in incorporating measures of overall environmental contamination into pollution studies. - Mercury levels in bioindicators at three trophic levels were combined using geostatistics to build an integrated environmental contamination index.

  15. Trees as bioindicator of heavy metal pollution in three European cities

    Energy Technology Data Exchange (ETDEWEB)

    Sawidis, T. [Department of Botany, University of Thessaloniki, 54124 Thessaloniki (Greece); Breuste, J., E-mail: juergen.breuste@sbg.ac.at [Department of Geography and Geology, University of Salzburg, 5010 Salzburg (Austria); Mitrovic, M.; Pavlovic, P. [Department of Ecology, Institute for Biological Research ' Sinisa Stankovic' , University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade (Serbia); Tsigaridas, K. [Department of Botany, University of Thessaloniki, 54124 Thessaloniki (Greece)

    2011-12-15

    Concentrations of four heavy metals were determined in tree leaves and bark collected from polluted and non-polluted areas of three European cities (Salzburg, Belgrade and Thessaloniki) for a comparative study. Platanus orientalis L. and Pinus nigra Arn., widespread in urban northern and southern Europe, were tested for their suitability for air quality biomonitoring. Leaves and barks were collected uniformly of an initial quantity of about 30 g of each sample. Analysis was accomplished by electrothermal atomic absorption spectrometry after total digestion. Site-dependent variations were found with the highest concentration level measured in Belgrade, followed by Thessaloniki and Salzburg. A higher accumulation of heavy metals was found in bark compared to leaves. Pine tree bark, accumulating higher concentrations of trace metals compared to plane tree bark, shows a higher efficiency as bioindicator for urban pollution. Both indicator species are suitable for comparative studies on bioindication of urban air pollution. - Highlights: > Oriental plane and Austrian pine are suitable for comparative urban air quality biomonitoring of heavy metal pollution. > Pine tree is excellently suitable as urban bioindicator as it accumulates high concentrations of trace metals. > The highest heavy metal pollution was found in Belgrade followed by Thessaloniki and Salzburg. - Oriental plane (Platanus orientalis L.) and Austrian pine (Pinus nigra Arn.), widespread in urban northern and southern Europe, are suitable for comparative biomonitoring of urban air pollution.

  16. An approach for assessment of water quality using semipermeable membrane devices (SPMDs) and bioindicator tests

    Science.gov (United States)

    Petty, J.D.; Jones, S.B.; Huckins, J.N.; Cranor, W.L.; Parris, J.T.; McTague, T.B.; Boyle, T.P.

    2000-01-01

    As an integral part of our continued development of water quality assessment approaches, we combined integrative sampling, instrumental analysis of widely occurring anthropogenic contaminants, and the application of a suite of bioindicator tests as a specific part of a broader survey of ecological conditions, species diversity, and habitat quality in the Santa Cruz River in Arizona, USA. Lipid-containing semipermeable membrane devices (SPMDs) were employed to sequester waterborne hydrophobic chemicals. Instrumental analysis and a suite of bioindicator tests were used to determine the presence and potential toxicological relevance of mixtures of bioavailable chemicals in two major water sources of the Santa Cruz River. The SPMDs were deployed at two sites; the effluent weir of the International Wastewater Treatment Plant (IWWTP) and the Nogales Wash. Both of these systems empty into the Santa Cruz River and the IWWTP effluent is a potential source of water for a constructed wetland complex. Analysis of the SPMD sample extracts revealed the presence of organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs). The bioindicator tests demonstrated increased liver enzyme activity, perturbation of neurotransmitter systems and potential endocrine disrupting effects (vitellogenin induction) in fish exposed to the extracts. With increasing global demands on limited water resources, the approach described herein provides an assessment paradigm applicable to determining the quality of water in a broad range of aquatic systems.

  17. Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action.

    Science.gov (United States)

    Batista, P F; Costa, A C; Megguer, C A; Lima, J S; Silva, F B; Guimarães, D S; Almeida, G M; Nascimento, K J T

    2017-10-23

    In Brazil, the expansion of agricultural activity and the associated indiscriminate use of herbicides such as glyphosate is directly related to the loss of biodiversity in the Cerrado. The identification of plant species as bioindicators of herbicide action, especially species native to the area, can help in monitoring the impacts of xenobiotics in the remaining Cerrado. Thus, this study was designed to evaluate the possible use of the native Cerrado species Pouteria torta as a bioindicator of glyphosate action via changes in physiological performance. At 16 months after sowing, the effect of glyphosate was evaluated by applying the following doses: 0 (control), 25, 50, 100, 200, 400, 800, and 1200 g a.e. ha-1. In response to glyphosate, P. torta exhibited reductions in photosynthesis and chloroplastid pigment content, as well as accumulation of shikimic acid and the occurrence of chlorosis and necrosis. These changes demonstrate the high sensitivity of P. torta to glyphosate and its potential for use as a bioindicator of this herbicide.

  18. Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action

    Directory of Open Access Journals (Sweden)

    P. F. Batista

    2017-10-01

    Full Text Available Abstract In Brazil, the expansion of agricultural activity and the associated indiscriminate use of herbicides such as glyphosate is directly related to the loss of biodiversity in the Cerrado. The identification of plant species as bioindicators of herbicide action, especially species native to the area, can help in monitoring the impacts of xenobiotics in the remaining Cerrado. Thus, this study was designed to evaluate the possible use of the native Cerrado species Pouteria torta as a bioindicator of glyphosate action via changes in physiological performance. At 16 months after sowing, the effect of glyphosate was evaluated by applying the following doses: 0 (control, 25, 50, 100, 200, 400, 800, and 1200 g a.e. ha-1. In response to glyphosate, P. torta exhibited reductions in photosynthesis and chloroplastid pigment content, as well as accumulation of shikimic acid and the occurrence of chlorosis and necrosis. These changes demonstrate the high sensitivity of P. torta to glyphosate and its potential for use as a bioindicator of this herbicide.

  19. Effects of Orthosiphon grandiflorus, Hibiscus sabdariffa and Phyllanthus amarus extracts on risk factors for urinary calcium oxalate stones in rats.

    Science.gov (United States)

    Woottisin, Surachet; Hossain, Rayhan Zubair; Yachantha, Chatchai; Sriboonlue, Pote; Ogawa, Yoshihide; Saito, Seiichi

    2011-01-01

    We evaluated the antilithic effect of Orthosiphon grandiflorus, Hibiscus sabdariffa and Phyllanthus amarus extracts on known risk factors for calcium oxalate stones in rats. We divided 30 male Wistar rats into 5 equal groups. Controls were fed a standard diet and the remaining groups received a 3% glycolate diet for 4 weeks to induce hyperoxaluria. One glycolate fed group served as the untreated group and the others were given oral extracts of Orthosiphon grandiflorus, Hibiscus sabdariffa or Phyllanthus amarus at a dose of 3.5 mg daily. We collected 24-hour urine and blood samples. Kidneys were harvested for histological examination. We measured the renal tissue content of calcium and oxalate. The Hibiscus sabdariffa group showed significantly decreased serum oxalate and glycolate, and higher oxalate urinary excretion. The Phyllanthus amarus group showed significantly increased urinary citrate vs the untreated group. Histological examination revealed less CaOx crystal deposition in the kidneys of Hibiscus sabdariffa and Phyllanthus amarus treated rats than in untreated rats. Those rats also had significantly lower renal tissue calcium content than untreated rats. All parameters in the Orthosiphon grandiflorus treated group were comparable to those in the untreated group. Hibiscus sabdariffa and Phyllanthus amarus decreased calcium crystal deposition in the kidneys. The antilithic effect of Hibiscus sabdariffa may be related to decreased oxalate retention in the kidney and more excretion into urine while that of Phyllanthus amarus may depend on increased urinary citrate. In contrast, administering Orthosiphon grandiflorus had no antilithic effect. Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  20. Hibiscus sabdariffa calyx palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in fructose-induced metabolic syndrome rats.

    Science.gov (United States)

    Ajiboye, Taofeek O; Raji, Hikmat O; Adeleye, Abdulwasiu O; Adigun, Nurudeen S; Giwa, Oluwayemisi B; Ojewuyi, Oluwayemisi B; Oladiji, Adenike T

    2016-03-30

    The effect of Hibiscus sabdariffa calyx extract was evaluated in high-fructose-induced metabolic syndrome rats. Insulin resistance, hyperglycemia, dyslipidemia and oxidative rout were induced in rats using high-fructose diet. High-fructose diet-fed rats were administered 100 and 200 mg kg(-1) body weight of H. sabdariffa extract for 3 weeks, starting from week 7 of high-fructose diet treatment. High-fructose diet significantly (P Hibiscus extract. Overall, aqueous extract of H. sabdariffa palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in high-fructose-induced metabolic syndrome rats. © 2015 Society of Chemical Industry.

  1. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Science.gov (United States)

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  2. THE ANTIBACTERIAL EFFECT OF ROSELLE (HIBISCUS SABDARIFFA EXTRACT AGAINST STAPHYLOCOCCUS EPIDERMIDIS IN VITRO

    Directory of Open Access Journals (Sweden)

    Terrence Timothy Evan Lusida

    2017-04-01

    Full Text Available Infection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa, a well known traditional medicine plant, is a potential candidate as a drug againts infectious disease. The purpose of this research is to investigate the antibacterial effect of ethanol extract from Roselle (Hibiscus sabdariffa calyx againts the growth of Staphylococcus epidermidis. Assessment for antibacterial effect is performed using broth diffusion method. The extract is made by maceration of the calyx of Roselle in 96% ethanol. Extracts with concentration of 125, 62.5, 31.25, 15.63, 7.81, 3.90, 1.95, 0.97, 0.48, 0.24 mg/mL are added into separated Mueller-Hinton broths (MHB, which have already been inoculated by Staphylococcus epidermidis. As for bacterial growth control, we used MHB with bacterial inoculation, while sterility control we used mixture of extract and MHB. Then from each broth, the solutions are added into separated nutrition agar plates. Replications are done three times. Clarity and bacterial growth are observed after 24 hours of incubation. However, clarity cannot be observed in 36 broth, but bacterial growth is observed on the plate for concentration 0.97, 0.48, and 0.24 mg/mL. Therefore Minimum inhibitory concentration (MIC cannot be determined because the extract’s color interfere the observation. While minimum bactericidal concentration (MBC, the last concentration before the concentration where the bacteria are still viable, is 1.95 mg/mL. Based on the result of the research, the Roselle calyx ethanol extract (Hibiscus sabdariffa through dilution method with a concentration of 1.95 mg / mL can kill Staphylococcus epidermidis and in order to find MIC in collored and turbid solution (before being incubated in incubator, we can consider using agar dilution methode or microdilution methode.

  3. Metabolic profile and hepatoprotective activity of the anthocyanin-rich extract of Hibiscus sabdariffa calyces.

    Science.gov (United States)

    Ezzat, Shahira M; Salama, Maha M; Seif El-Din, Sayed H; Saleh, Samira; El-Lakkany, Naglaa M; Hammam, Olfat A; Salem, Maha B; Botros, Sanaa S

    2016-12-01

    Hibiscus sabdariffa L. (Malvaceae) is a common traditional tea that has many biological activities. To evaluate the hepatoprotective effect and study the metabolic profile of the anthocyanin-rich extract of H. sabdariffa calyces (HSARE). The hepatoprotective activity of HSARE was assessed (100 mg/kg/d for 4 weeks) by examining the hepatic, inflammatory, oxidative stress markers and performing a histopathological examination in rats with thioacetamide (TAA)-induced hepatotoxicity. HSARE was analyzed using ultra-performance liquid chromatography-quadrupole-time-of-flight-photodiode array-mass spectrometry (UPLC-qTOF-PDA-MS). The UPLC-qTOF-PDA-MS analysis of HSARE enabled the identification of 25 compounds represented by delphinidin and its derivatives, cyanidin, kaempferol, quercetin, myricetin aglycones and glycosides, together with hibiscus lactone, hibiscus acid and caffeoylquinic acids. Compared to the TAA-intoxicated group, HSARE significantly reduced the serum levels of alanine aminotransferase, aspartate aminotransferase and hepatic malondialdehyde by 37.96, 42.74 and 45.31%, respectively. It also decreased hepatic inflammatory markers, including tumour necrosis factor alpha, interleukin-6 and interferon gamma (INF-γ), by 85.39, 14.96 and 70.87%, respectively. Moreover, it decreased the immunopositivity of nuclear factor kappa-B and CYP2E1 in liver tissue, with an increase in the effector apoptotic marker (caspase-3 positive cells), restoration of the altered hepatic architecture and increases in the activities of superoxide dismutase (SOD) and glutathione by 150.08 and 89.23%, respectively. HSARE revealed pronounced antioxidant and anti-inflammatory potential where SOD and INF-γ were significantly improved. HSARE possesses the added value of being more water-soluble and of natural origin with fewer side effects expected compared to silymarin.

  4. Usage of immobilized porcine pancreas lipase in the hydrolysis of roselle (Hibiscus sabdariffa L.) seed oil

    Science.gov (United States)

    Ai, Chau Tran Diem; Linh, Vo Thi Hong; Yen, Tran Thi Ngoc; Nguyen, Nguyen Thi; Hoa, Phan Ngoc

    2017-09-01

    This study focused on the comparison among the usage of immobilized porcine pancreas lipase (PPL) on different hydrotalcite carriers (uncalcined and calcined hydrotalcite - like compound Mg /Al) and free lipase as the catalysts to hydrolyze of roselle (Hibiscus sabdariffa L.) seed oil. The reaction conditions were investigated including the ratio of oil to buffer, ratio of enzyme to substrate, the temperature of the hydrolysis, pH. The calcined hydrotalcite showed a higher lipase immobilization yield and a better reusability than the uncalcined hydrotalcite (87.15% and 86.78%, respectively).

  5. Extraction of lipid components from hibiscus seeds by supercritical carbon dioxide and ethanol mixtures

    Energy Technology Data Exchange (ETDEWEB)

    Holser, Ronald A.; King, J. W. (Jerry W.); Bost, G.

    2002-01-01

    The genus Hibiscus exhibits great diversity in the production of natural materials with edible and industrial applications. The seeds of twelve varieties of Hibiscus were investigated as a source for triglycerides and phospholipids that could be used in functional foods. Lipid components were extracted from seed samples ground to a nominal particle diameter of 0.1 mm. Extractions were performed with an ISCO model 3560 supercritical fluid extractor using carbon dioxide and a mixture of carbon dioxide modified with ethanol. The neutral lipids were extracted with carbon dioxide at 80 C and 5370 MPa for 45 min. Polar lipids were subsequently extracted with a mixture of carbon dioxide and 15% ethanol at the same temperature and pressure. High performance liquid chromatography (HPLC) was used to analyze extracts for major neutral and polar lipid classes. A silica column was used with a solvent gradient of hexane/isopropanol/ water and ultraviolet (UV) and evaporative light scattering detectors (ELSD). An aliquot of each triglyceride fraction was trans-methylated with sodium methoxide and analyzed by gas chromatography to obtain the corresponding fatty acid methyl esters. The total lipids extracted ranged from 8.5% for a variety indigenous to Madagascar (H. calyphyllus) to 20% for a hybrid species (Georgia Rose). The average oil yield was 11.4% for the other varieties tested. The fatty acid methyl ester analysis displayed a high degree of unsaturation for all varieties tested, e. g., 75 ' 83%. Oleic, linoleic, and linolenic fatty acids were the predominate unsaturated fatty acids with only minor amounts of C14, C18, and C20 saturated fatty acids measured. Palmitic acid was identified as the predominate saturated fatty acid. The distribution of the major phospholipids, i. e., phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylcholine, and lysophosphatidylcholine, was found to vary significantly among the hibiscus species examined

  6. Post-Coital Antifertility Activity of Hibiscus rosa-sinensis Linn. Roots

    Directory of Open Access Journals (Sweden)

    Neeru Vasudeva

    2008-01-01

    Full Text Available Ancient literature mentions the use of a number of plants/preparations for fertility regulation. Some local contraceptive agents have also been described in Ayurvedic and Unani texts. Documented experiments or clinical data are, however, lacking. Therefore, the present study was undertaken to explore the antifertility and estrogenic activity of ethanolic extract of the roots of Hibiscus rosa-sinensis Linn. A strong anti-implantation (inhibition 100% and uterotropic activity was observed at the dose level of 400 mg/kg body weight. Histological studies were carried out to confirm this effect.

  7. Effet de l'incorporation de graines d' Hibiscus Sabdariffa (Oseille de ...

    African Journals Online (AJOL)

    Une étude de l'effet de l'incorporation des graines d'Hibiscus Sabdariffa L. dans la ration alimentaire sur quelques paramètres d'ingestion et de croissance du poulet en aviculture traditionnelle améliorée a été conduite sur 120 animaux au Burkina Faso. Le maïs, source habituelle d'énergie et de protéines de la ration, a été ...

  8. Impact of kenaf (Hibiscus cannabinus L.) leaf, bark, and core extracts on germination of five plant species

    Science.gov (United States)

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the germination and post-germination development ...

  9. Field evaluation of two systemic neonicotinoid insecticides against pink hibiscus mealybug (Maconellicoccus hirsutus (Green))on mulberry trees

    Science.gov (United States)

    Infestations of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green), in ornamental trees were already in an advanced state at the time of its discovery in the Imperial Valley of California (USA) in August 1999. Concern about the spread of M. hirsutus beyond the Imperial Valley led to the p...

  10. Characterization of developmental and stress mediated expression of cinnamoyl-CoA reductase (CCR) in kenaf (Hibiscus cannabinus L.)

    Science.gov (United States)

    Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), enco...

  11. Investigation of the bacterial retting community of kenaf (Hibiscus cannabinus) under different conditions using next-generation semiconductor sequencing

    Science.gov (United States)

    The use of the natural fibers requires the development of cost-efficient processing of fibers with consistent, uniform properties. The microbial communities associated with kenaf (Hibiscus cannabinus) plant fibers during retting were determined in an effort to identify possible means of accelerating...

  12. Wilt, crown, and root rot of common rose mallow (Hibiscus moscheutos) caused by a novel Fusarium sp

    Science.gov (United States)

    A new crown and root rot disease of landscape plantings of the malvaceous ornamental common rose mallow (Hibiscus moscheutos) was first detected in Washington State in 2012. The main objectives of this study were to complete Koch's postulates, document the disease sypmtoms photographically, and iden...

  13. Molecular cloning, characterization and expression of the caffeic acid O-methyltransferase (COMT) ortholog from kenaf (Hibiscus cannabinus)

    Science.gov (United States)

    We cloned the full-length of the gene putatively encoding caffeic acid O-methyltransferase (COMT) from kenaf (Hibiscus cannabinus L.) using degenerate primers and the RACE (rapid amplification of cDNA ends) method. Kenaf is an herbaceous and rapidly growing dicotyledonous plant with great potential ...

  14. Genetic variability for phenotype, seed production, oil content, and fatty acid composition among 17 Roselle (Hibiscus sabdariffa) accessions

    Science.gov (United States)

    Seed oil and fatty acids in plants have human health implications. Oil from roselle (Hibiscus sabdariffa L.) seeds are used in Taiwan as a diuretic, laxative, and tonic. The objectives of this study were to evaluate seeds from 17 roselle accessions for oil and fatty acid variation in a greenhouse. S...

  15. Phytochemical, sensory attributes and aroma stability of dense phase carbon dioxide processed Hibiscus sabdariffa beverage during storage.

    Science.gov (United States)

    Ramírez-Rodrigues, Milena M; Plaza, Maria L; Azeredo, Alberto; Balaban, Murat O; Marshall, Maurice R

    2012-10-01

    The effect of dense phase carbon dioxide (DPCD) processing (34.5 MPa, 8% CO₂, 6.5 min, and 40 °C) on phytochemical, sensory and aroma compounds of hibiscus beverage was compared to a conventional thermal process (HTST) (75 °C for 15 s) and a control (untreated beverage) during refrigerated storage (4 °C). The overall likeability of the hibiscus beverage for all treatments was not affected by storage up to week 5. DPCD process retained more aroma volatiles as compared to HTST. Aroma profiles in the beverages were mainly composed of alcohols and aldehydes with 1-octen-3-ol, decanal, octanal, 1-hexanol, and nonanal as the compounds with the highest relative percentage peak areas. A loss of only 9% anthocyanins was observed for the DPCD processed hibiscus beverage. Phytochemical profiles in the hibiscus beverage included caffeoylquinic acids, anthocyanins, and flavonols. No major changes in total phenolics and antioxidant capacity occurred during the 14 weeks of storage. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Multi-locus DNA barcoding identifies matK as a suitable marker for species identification in Hibiscus L.

    Science.gov (United States)

    Poovitha, Sundar; Stalin, Nithaniyal; Balaji, Raju; Parani, Madasamy

    2016-12-01

    The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2. No intraspecies divergence was observed among the accessions studied. Interspecies divergence was 0%-9.6% with individual markers, which increased to 0%-12.5% and 0.8%-20.3% when using two- and three-marker combinations, respectively. Differentiation of all the species of Hibiscus was possible with the matK DNA barcode marker. Also, in two-marker combinations, only those combinations with matK differentiated all the species. Though all the three-marker combinations showed 100% species differentiation, species resolution was consistently better when the matK marker formed part of the combination. These results clearly showed that matK is more suitable when compared to rbcLa, trnH-psbA, and ITS2 for species identification in Hibiscus.

  17. The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. 'Nairobi'

    NARCIS (Netherlands)

    Meeteren, van U.; Gelder, van A.

    2000-01-01

    When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. 'Nairobi' plants to red light (635 to 685 nm, 2.9 μmol?m-2?s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, μmol?m-2?s-1) accelerated this process. Flower bud abscission in response to

  18. Growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) under different agricultural inputs and management practices in central Greece

    NARCIS (Netherlands)

    Danalatos, N.G.; Archontoulis, S.V.

    2010-01-01

    The growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) cultivars Tainung 2 and Everglades 41 were determined under three irrigation applications (low: 25%, moderate: 50% and fully: 100% of maximum evapotranspiration; ETm), four nitrogen dressings (0, 50, 100 and 150 kg hat), two

  19. Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth

    Directory of Open Access Journals (Sweden)

    Karina H. Goldberg

    2017-01-01

    Full Text Available Skin cancer is extremely common, and melanoma causes about 80% of skin cancer deaths. In fact, melanoma kills over 50 thousand people around the world each year, and these numbers are rising. Clearly, standard treatments are not effectively treating melanoma, and alternative therapies are needed to address this problem. Hibiscus tea has been noted to have medicinal properties, including anticancer effects. Extracts from Hibiscus have been shown to inhibit the growth of a variety of cancer cells. In particular, recent studies found that polyphenols extracted from Hibiscus sabdariffa by organic solvents can inhibit melanoma cell growth. However, effects of aqueous extracts from Hibiscus rosa-sinesis flowers, which are commonly used to make traditional medicinal beverages, have not been examined on melanoma cells. Here, we report that aqueous H. rosa-sinesis flower extract contains compounds that inhibit melanoma cell growth in a dose dependent manner at concentrations that did not affect the growth of nontransformed cells. In addition, these extracts contain low molecular weight growth inhibitory compounds below 3 kD in size that combine with larger compounds to more effectively inhibit melanoma cell growth. Future work should identify these compounds, and evaluate their potential to prevent and treat melanoma and other cancers.

  20. In Vitro Digestibility of Aluminum from Hibiscus sabdariffa Hot Watery Infusion and Its Concentration in Urine of Healthy Individuals

    Czech Academy of Sciences Publication Activity Database

    Frankova, A.; Malik, J.; Drabek, O.; Szakova, J.; Sperlingova, I.; Kloucek, P.; Novy, P.; Tejnecky, V.; Landa, Přemysl; Leuner, O.; Kokoska, L.

    2016-01-01

    Roč. 174, č. 2 (2016), s. 267-273 ISSN 0163-4984 Institutional support: RVO:61389030 Keywords : dialysis dementia * tea * bioavailability * speciation * toxicity * Aluminum * In vitro digestion * Hot watery infusion * Urine * Hibiscus sabdariffa L Subject RIV: EF - Botanics Impact factor: 2.399, year: 2016

  1. Are Mussels Always the Best Bioindicators? Comparative Study on Biochemical Responses of Three Marine Invertebrate Species to Chronic Port Pollution.

    Science.gov (United States)

    Laitano, María V; Fernández-Gimenez, Analía V

    2016-07-01

    Bivalves have traditionally been considered good bioindicators due to their sensitivity to pollution, among other features. This characteristic is shared by several other non-bivalve species as well, though studies in this respect remain scarce. This work aims to compare biomarker sensitivity to chronic port pollution among three intertidal invertebrate species with good bioindicator characteristics. Mussels' immunological (phenoloxidase and peroxidases) and biotransformation (glutathione-S-transferase) responses were contrasted against those of limpets and barnacles. The three species under study evidenced activity of all the enzymes measured, although with differences. Barnacle Balanus glandula was the most sensitive species showing pollution modulation of the three enzymes, which suggests that mussels would not always be the best bioindicator species among marine invertebrates depending on the responses that are assessed.

  2. Bioindicators for monitoring marine litter ingestion and its impacts on Mediterranean biodiversity.

    Science.gov (United States)

    Fossi, Maria Cristina; Pedà, Cristina; Compa, Montserrat; Tsangaris, Catherine; Alomar, Carme; Claro, Francoise; Ioakeimidis, Christos; Galgani, Francois; Hema, Tatjana; Deudero, Salud; Romeo, Teresa; Battaglia, Pietro; Andaloro, Franco; Caliani, Ilaria; Casini, Silvia; Panti, Cristina; Baini, Matteo

    2017-11-16

    The Mediterranean Sea has been described as one of the most affected areas by marine litter in the world. Although effects on organisms from marine plastic litter ingestion have been investigated in several oceanic areas, there is still a lack of information from the Mediterranean Sea. The main objectives of this paper are to review current knowledge on the impact of marine litter on Mediterranean biodiversity, to define selection criteria for choosing marine organisms suitable for use as bioindicator species, and to propose a methodological approach to assessing the harm related to marine litter ingestion in several Mediterranean habitats and sub-regions. A new integrated monitoring tool that would provide the information necessary to design and implement future mitigation actions in the Mediterranean basin is proposed. According to bibliographic research and statistical analysis on current knowledge of marine litter ingestion, the area of the Mediterranean most studied, in terms of number of species and papers in the Mediterranean Sea is the western sub-area as well as demersal (32.9%) and pelagic (27.7%) amongst habitats. Applying ecological and biological criteria to the most threatened species obtained by statistical analysis, bioindicator species for different habitats and monitoring scale were selected. A threefold approach, simultaneously measuring the presence and effects of plastic, can provide the actual harm and sub-lethal effects to organisms caused by marine litter ingestion. The research revealed gaps in knowledge, and this paper suggests measures to close the gap. This and the selection of appropriate bioindicator species would represent a step forward for marine litter risk assessment, and the implementation of future actions and mitigation measures for specific Mediterranean areas, habitats and species affected by marine litter ingestion. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Bioindicator value of flagellates in urban wastewater treatment using membrane bioreactors.

    Science.gov (United States)

    Parada-Albarracín, J A; Pérez, J; Gómez, M A

    2017-10-01

    Two experimental submerged membrane bioreactors (MBR) at the industrial scale with different membranes (microfiltration and ultrafiltration) were used to analyse the influence of the small flagellates on the lack of significance of the sludge biotic index (SBI). Also, with these systems, flagellates were analysed as bioindicators in the activated sludge of MBRs. Both facilities were operated in parallel with a pre-denitrification system for a period of nearly two years and fed with real pretreated urban wastewater. During the experimental period, the sludge-retention time in the facilities was from 16.5 to 36.5 days, the organic loading ranged between 1.8 Kg COD/d m 3 and 0.14 Kg COD/d m 3 , and the temperature of the activated sludge was between 32.8 and 12.8 °C. Under these conditions, the concentrations of total solids in the activated sludge reached 21.6 and 0.3 g/L, and the ratio of food:microrganims (F:M) remained stable at around 0.1 Kg BOD 5 /Kg VSS day, while the trans-membrane pressure (TMP) followed an increasing trend. Both facilities presented a high capacity for organic matter elimination and complete nitrification. The small flagellates were present throughout the experimental period in the activated sludge and showed no significant bioindicator role in the MBR systems which limited the application of SBI. Of the large flagellates studied, only Peranema sp. could be considered to be a bioindicator of MBR systems, indicating the effectiveness of denitrification for systems with complete nitrification and correlating with the time course of the TMP. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. LEAF-CUTTING ANTS Acromyrmex niger SMITH, 1858 (HYMENOPTERA; FORMICIDAE) USED AS BIOINDICATORS OF AGROTOXICS RESIDUES

    OpenAIRE

    Liriana Belizário Cantagalli; Denise Alves Lopes; Ana Lúcia Paes Barateiro Stuchi; Maria Claudia Colla Ruvolo Takasusuki

    2014-01-01

    Despite the condition of leaf-cutting ant pests in agroecosystems, it is undeniable the benefits they can bring in certain situations or environments. The leaf-cutting ants of the genus Acromyrmex attack mainly leaves of vegetables and fruit trees exposing not only to the agrochemicals used for their control as well as to those used for the control of other pests. Due to the bioindicator potential of environmental quality of the ants and their frequent exposure to agrochemicals such as organo...

  5. The snow vole (Chionomys nivalis) as an appropriate environmental bioindicator in alpine ecosystems

    Energy Technology Data Exchange (ETDEWEB)

    Metcheva, Roumiana [Institute of Zoology, Bulgarian Academy of Science, 1, Tzar Osvoboditel blvd., Sofia, 1000 Bulgaria (Bulgaria)], E-mail: rummech@yahoo.com; Beltcheva, Michaela; Chassovnikarova, Tsenka [Institute of Zoology, Bulgarian Academy of Science, 1, Tzar Osvoboditel blvd., Sofia, 1000 Bulgaria (Bulgaria)

    2008-03-01

    The snow vole (Chionomys nivalis, Martins, 1842) is a common species in the Bulgarian high mountains. Its populations are distributed in different altitudes, regions, and keep stable population density. This is the reason the species has been tested as a bioindicator for environmental quality in alpine ecosystems. The cumulative environmental impact in snow vole populations was evaluated using cytogenetical, hematological, ecotoxicological, radiometrical, ecophysiological, and morphophysiological indices. Standard karyotype, chromosomal aberrations, and other diversions have been observed. These investigations reveal that the snow vole is one of the most appropriate species that can be used as a biomonitor for environmental assessment in mountain areas.

  6. Yeast-like fungi possessing bio-indicator properties isolated from the Łyna river

    Directory of Open Access Journals (Sweden)

    Maria Dynowska

    2014-08-01

    Full Text Available Yeast-like fungi isolated in the Łyna river are constant components of microflora of inland waters. Every increase in their number indicates progress in the process of eutrophication and accumulation of organic and inorganic pollutans. The fungi Candida aibicans, Pichia guilliermondii, P. anomala, Rhodotorula glutinis i Trichosporon beigelii, potentially pathogenic apperred in water with high content of municipal sewage, but T. aquatile - in the clean waters only. The tested fungi can be also considered as bio-indicators.

  7. Antibiotic Resistant Bacteria As Bio-Indicator Of Polluted Effluent In The Green Turtles, In Oman

    OpenAIRE

    Al-Bahry, Saif N.; Mahmoud, Ibrahim Y.; Al-Zadjali, Maheera; Elshafie, Abdulkader; Al-Harthy, Asila; Al-Alawi, Wafaa

    2011-01-01

    Abstract Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A non-invasive procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolde...

  8. Study of ants as bioindicators of industrial pollution in Kemerovo Region, Russia

    Science.gov (United States)

    Blinova, S. V.; Dobrydina, T. I.

    2018-01-01

    The myrmecocomplexes of five industrial cities in the territory of Kuzbass, Russia, were studied. The general trends in the reaction of ants to the pollution by gaseous (chemical industry) and solid (coal and cement dust, lead and zinc waste and metallurgical industry) emissions were revealed. It was found out that the proportion of species of subfamilies, species richness and density of the settlement can be used as bioindicators. The predominance of L. niger and M. rubra nests in myrmecocomplexes immediately indicates a high degree of man-made impact.

  9. Lichens as bioindicators of air quality in Dimitrovgrad (South-Eastern Serbia

    Directory of Open Access Journals (Sweden)

    Stamenković S.

    2010-01-01

    Full Text Available Air pollution detection in Dimitrovgrad has not been done yet. In this work different lichen have been used as a bioindication to establish different air pollution levels. At 18 investigated points 22 lichen taxa have been found. Using the Index of Atmospheric Purity (IAP it has been found that there are 3 different air pollution zones in Dimitrovgrad: 'lichen desert', 'transitional' and 'normal zone'. The most sensitive lichen taxa in Dimitrovgrad are Evernia prunastra, Ochrolechia pallescens, Parmelia sulcata, and Physcia tenella and the most tolerant are Phaeophyscia orbicularis, Physcia adscendens, Physconia distorta, Physconia grisea, and Xanthoria parietina. .

  10. Bioindication and ecological rate setting with radioecology taken as an example

    International Nuclear Information System (INIS)

    Krivolutskij, D.A.; Tikhomirov, F.A.; Fedorov, E.A.; Smirnov, E.G.; Moskovskij Gosudarstvennyj Univ.

    1986-01-01

    Necessity in ecological rate setting of technogenic contaminations (including radionuclides) in topsoil and soils is substantiated. Bioindication of technogenic contaminations with provision for community of distribution and migration of contaminating substances in topsoil and their effect on organisms, populations and biocenoses is suggested as one of approaches to ecological rate setting. General problems of methodology and objects of ecological rate setting in phytocenoses and zoocenoses are discussed. Basic principles and criteria of rate setting of contaminating substance content in soils are considered with provision for regional soil-climatic conditions and contaminating substance properties

  11. Representative benthic bioindicator organisms for use in radiation effects research: Culture of Neanthes arenaceodentata (Polychaeta)

    International Nuclear Information System (INIS)

    Harrison, F.L.; Knezovich, J.P.; Martinelli, R.E.

    1992-09-01

    The purpose of this document is to present a comprehensive synthesis of information pertaining to the selection and maintenance of bioindicator organisms for use in radiation-effects research. The focus of this report is on the benthic polychaete, Neanthes arenaceodentata, a species that has been used successfully at the Lawrence Livermore National Laboratory (LLNL) and other institutions to define the impacts of radiation and chemical toxicants on aquatic organisms. In this document, the authors provide a rationale for the selection of this organism, a description of its reproductive biology, and a description of the conditions that are required for the maintenance and rearing of the organism for use in toxicological research

  12. Hibiscus sabdariffa leaf polyphenolic extract induces human melanoma cell death, apoptosis, and autophagy.

    Science.gov (United States)

    Chiu, Chun-Tang; Hsuan, Shu-Wen; Lin, Hui-Hsuan; Hsu, Cheng-Chin; Chou, Fen-Pi; Chen, Jing-Hsien

    2015-03-01

    Melanoma is the least common but most fatal form of skin cancer. Previous studies have indicated that an aqueous extract of Hibiscus sabdariffa leaves possess hypoglycemic, hypolipidemic, and antioxidant effects. In this study, we want to investigate the anticancer activity of Hibiscus leaf polyphenolic (HLP) extract in melanoma cells. First, HLP was exhibited to be rich in epicatechin gallate (ECG) and other polyphenols. Apoptotic and autophagic activities of HLP and ECG were further evaluated by DAPI stain, cell-cycle analysis, and acidic vascular organelle (AVO) stain. Our results revealed that both HLP and ECG induced the caspases cleavages, Bcl-2 family proteins regulation, and Fas/FasL activation in A375 cells. In addition, we also revealed that the cells presented AVO-positive after HLP treatments. HLP could increase the expressions of autophagy-related proteins autophagy-related gene 5 (ATG5), Beclin1, and light chain 3-II (LC3-II), and induce autophagic cell death in A375 cells. These data indicated that the anticancer effect of HLP, partly contributed by ECG, in A375 cells. HLP potentially could be developed as an antimelanoma agent. © 2015 Institute of Food Technologists®

  13. A distributional and cytological survey of the presently recognized taxa of Hibiscus section Furcaria (Malvaceae.

    Directory of Open Access Journals (Sweden)

    F. Douglas Wilson

    2006-01-01

    Full Text Available Hibiscus section Furcaria is a natural group of plants that presently includes 109 recognized taxa. Taxa are found in subsaharan Africa, India, southeastern Asia, Malesia, Australia, islands of the Pacific basin, the Caribbean, North, Central, and South America. The basic chromosome number is x = 18. In nature, ploidy levels range from diploid to decaploid. The taxa exhibit a remarkable amount of genome diversity. At least 13 genomes have been identified, some distributed widely and others with more restricted distributions. No modern taxonomic monograph ofHibiscus section Furcaria exists, but a number of regional studies have appeared that are essentially global in extent. Also, a number of studies of chromosome numbers and genome relationships have been published. The present paper includes a census of all the presently accepted taxa, the geographical distribution of each taxon, and chromosome numbers and genome designations of the 49 taxa for which the information is available. Important mechanisms of speciation include genome divergence at the diploid level, followed by hybridization and allopolyploidy, significant species radiation at the tetraploid and hexaploid levels, and the development of even higher levels of allopolyploids.

  14. [Therapeutic potential of Hibiscus sabdariffa: a review of the scientific evidence].

    Science.gov (United States)

    Guardiola, Soledad; Mach, Núria

    2014-05-01

    Infusion of Hibiscus sabdariffa (H. sabdariffa) is a very popular drink in many parts of the world. Its phytochemical composition is associated to antioxidant, hypotensive, and antiatherosclerotic effects. However, the molecular mechanisms involved in these processes are not well known. The aim of this review was to report the scientific evidence supporting that regular use of H. sabdariffa decreases oxidative stress, atherosclerosis, lipid profile, and blood pressure. A search of recent publications was made in the following specialized electronic databases: Elsevier Journal, SciELO, FSTA, Science Direct, Springer Link, and NCBI. Results of research conducted in clinical trials in humans and in animal models and cell cultures were recorded. Keywords used included Hibiscus sabdariffa, oxidative stress, polyphenols, hypertension, atherosclerosis, and lipid profile. Results of the different articles suggested a possible therapeutic effect of H. sabdariffa extracts on oxidative stress, lipid profile, hypertension, and atherosclerosis thanks to its composition rich in phenolic compounds. Anthocyanins significantly decrease LDL oxidation, inhibit adipogenesis by regulating adipogenic signaling pathways and transcription factors, and modulate gene expression of certain microRNAs. No adverse events or side effects were reported. Further more homogeneous, placebo-controlled studies in humans are needed to state that H. sabdariffa has therapeutic efficacy in humans. Copyright © 2013 SEEN. Published by Elsevier Espana. All rights reserved.

  15. Determination of suitable microspore stage and callus induction from anthers of kenaf (Hibiscus cannabinus L.).

    Science.gov (United States)

    Ibrahim, Ahmed Mahmood; Kayat, Fatimah Binti; Hussin, Zeti Ermiena Surya Mat; Susanto, Dwi; Ariffulah, Mohammed

    2014-01-01

    Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6-8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.

  16. Polyphenolic extract from Hibiscus sabdariffa reduces body fat by inhibiting hepatic lipogenesis and preadipocyte adipogenesis.

    Science.gov (United States)

    Kao, Erl-Shyh; Yang, Mon-Yuan; Hung, Chia-Hung; Huang, Chien-Ning; Wang, Chau-Jong

    2016-01-01

    Diets high in fat lead to excess lipid accumulation in adipose tissue, which is a crucial factor in the development of obesity, hepatitis, and hyperlipidemia. In this study, we investigated the anti-obesity effect of Hibiscus sabdariffa extract (HSE) in vivo. Hamsters fed a high-fat diet (HFD) develop symptoms of obesity, which were determined based on body weight changes and changes in plasma and serum triglycerides, free fatty acid concentrations, total cholesterol levels, LDL-C levels, HDL-C levels, and adipocyte tissue weight. HFD-fed hamsters were used to investigate the effects of HSE on symptoms of obesity such as adipogenesis and fatty liver, loss of blood glucose regulation, and serum ion imbalance. Interestingly, HSE treatment effectively reduced the effects of the HFD in hamsters in a dose-dependent manner. Further, after inducing maturation of preadipocytes, Hibiscus sabdariffa polyphenolic extract (HPE) was shown to suppress the adipogenesis of adipocytes. However, HPE does not affect the viability of preadipocytes. Therefore, both HSE and HPE are effective and viable treatment strategies for preventing the development and treating the symptoms of obesity.

  17. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

    Science.gov (United States)

    2012-01-01

    Background Hibiscus tiliaceus L. (Malvaceae) is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect. PMID:22494845

  18. Chemical-sensory properties and consumer preference of hibiscus beverages produced by improved industrial processes.

    Science.gov (United States)

    Monteiro, Maria João P; Costa, Ana Isabel A; Fliedel, Geneviève; Cissé, Mady; Bechoff, Aurélie; Pallet, Dominique; Tomlins, Keith; Pintado, Maria Manuela E

    2017-06-15

    The need to increase sustainability and add value to traditional foods claiming health benefits led to the introduction of key improvements in the production of hibiscus beverages in Senegal. The physicochemical and sensory properties of three resulting products (an under-vacuum concentrate, a dilute-to-taste syrup and a ready-to-drink infusion) were assessed, vis-à-vis those of conventionally manufactured beverages, and their impact on local consumer preference determined (n=146). New beverages had more intense, redder colour and higher monomeric anthocyanin content, total phenolic content and antioxidant capacity. Moreover, their colour evaluations by trained panellists were mainly linked to colour density and anthocyanin/polyphenol content, while flavour assessments were associated to titratable acidity and sugar-to-acid ratio. Consumer evaluations, in turn, were driven by the beverages' red colour intensity, aroma strength and balance between sweetness and acidity. This explained why they overwhelmingly preferred the under-vacuum concentrate, regardless of their age, gender or frequency of hibiscus beverage consumption. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Anti-Escherichia coli effect of Hibiscus sabdariffa L. in a meat model

    Directory of Open Access Journals (Sweden)

    Marcelo Pinto PAIM

    Full Text Available Abstract Hibiscus sabdariffa L. is used in traditional medicine because of its bioactive properties, such as antioxidant and antibacterial. Escherichia coli is a Gram-negative bacteria and as an indicator of contamination in food. The aim of this work was to evaluate the anti-Escherichia coli effect and the change in pH on the control of aerobic mesophilic microorganisms, using hydroethanolic extract of H. sabdariffa L. in different concentrations in a meat model, verifying its potential as food additive for microbiological stability on ground beef during cooling storage. For the preparation of the treatments, the meat experimental units were elaborated with different concentrations of the vegetal extract (5, 10, 15 and 20%, ground beef and contaminated with E. coli. For pH evaluation, the meat experimental units were added different percentages of hydroethanolic extract. The H. sabdariffa L. antibacterial action reduced two logarithmic levels in practically all treatments. The best pH result was obtained in the meat containing 30% of the extract. The hydroethanolic extracts of Hibiscus sabdariffa L. showed anti-Escherichia coli activity in the presence of refrigerated ground beef. Analyzing the pH results and the count of aerobic mesophilic bacteria, it is possible this extract to be used as a natural food additive.

  20. Hibiscus sabdariffa (Roselle) Extracts and Wine: Phytochemical Profile, Physicochemical Properties, and Carbohydrase Inhibition.

    Science.gov (United States)

    Ifie, Idolo; Marshall, Lisa J; Ho, Peter; Williamson, Gary

    2016-06-22

    Three varieties of Hibiscus sabdariffa were analyzed for their phytochemical content and inhibitory potential on carbohydrate-digesting enzymes as a basis for selecting a variety for wine production. The dark red variety was chosen as it was highest in phenolic content and an aqueous extract partially inhibited α-glucosidase (maltase), with delphinidin 3-O-sambubioside, cyanidin 3-O-sambubioside, and 3-O-caffeoylquinic acid accounting for 65% of this activity. None of the varieties significantly inhibited α-amylase. Regarding Hibiscus sabdariffa wine, the effect of fermentation temperature (20 and 30 °C) on the physicochemical, phytochemical, and aroma composition was monitored over 40 days. The main change in phytochemical composition observed was the hydrolysis of 3-O-caffeolquinic acid and the concomitant increase of caffeic acid irrespective of fermentation temperature. Wine fermented at 20 °C was slightly more active for α-glucosidase inhibition with more fruity aromas (ethyl octanoate), but there were more flowery notes (2-phenylethanol) at 30 °C.

  1. In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta

    Science.gov (United States)

    Sarr, Mamadou; Ngom, Saliou; Kane, Modou O; Wele, Alassane; Diop, Doudou; Sarr, Bocar; Gueye, Lamine; Andriantsitohaina, Ramaroson; Diallo, Aminata S

    2009-01-01

    Background In this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal. Methods Vascular effects of crude extract of dried and powdered calyces of Hibiscus sabdariffa were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers. The crude extract was also enriched by liquid-liquid extraction. The various cyclohexane, dichloromethane, ethyl acetate, butanol extracts obtained as well as the residual marc were subjected to Sephadex LH-20 column chromatography. The different methanolic eluate fractions were then analyzed by Thin Layer (TLC) and High Performance Liquid Chromatography (HPLC) and their vascular effects also evaluated. Results The H. Sabdariffa crude extract induced mainly endothelium-dependent relaxant effects. The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels. The phytochemical analysis revealed the presence of phenolic acids in the ethyl acetate extract and anthocyans in the butanolic extract. The biological efficiency of the various studied extracts, in term of vasorelaxant capacity, showed that: Butanol extract > Crude extract > Residual marc > Ethyl acetate extract. These results suggest that the strong activity of the butanolic extract is essentially due to the presence of anthocyans found in its fractions 43-67. Conclusion These results demonstrate the vasodilator potential of hibiscus sabdariffa and contribute to his valuation as therapeutic alternative. PMID:19883513

  2. Ethyl acetate fraction from Hibiscus sabdariffa L. attenuates diabetes-associated cognitive impairment in mice.

    Science.gov (United States)

    Seung, Tae Wan; Park, Seon Kyeong; Kang, Jin Yong; Kim, Jong Min; Park, Sang Hyun; Kwon, Bong Seok; Lee, Chang Jun; Kang, Jeong Eun; Kim, Dae Ok; Lee, Uk; Heo, Ho Jin

    2018-03-01

    The ameliorating effects of the ethyl acetate fraction from Hibiscus sabdariffa L. (EFHS) 2 against diabetes mellitus (DM) 3 and DM-induced cognitive impairment were investigated on streptozotocin (STZ) 4 -induced DM mice. The EFHS groups showed improved hyperglycemia and glucose tolerance compared to the STZ group. Furthermore, their liver and kidney function and lipid metabolic imbalance in the blood serum were effectively recovered. The EFHS groups significantly ameliorated STZ-induced cognitive impairment in Y-maze, passive avoidance, and Morris water maze (MWM) 5 tests. The EFHS groups showed significant improvement in the antioxidant and cholinergic systems of the brain tissue. In addition, EFHS had an excellent ameliorating effect on protein expression levels from the tau hyperphosphorylation pathways, such as phospho-c-Jun N-terminal kinases (p-JNK), 6 phospho-tau (p-tau), 7 and cleaved poly (ADP-ribose) polymerase (c-PARP). 8 The main compounds of EFHS were identified as various phenolic compounds, including hibiscus acid, caffeoylquinic acid (CQA) 9 isomers, and quercetin derivates. Therefore, EFHS containing various physiologically active materials can potentially be used for improving DM-induced cognitive impairment via its antioxidant activity, improvement of the cholinergic system, and hyperphosphorylation tau signaling. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Anthocyanin-rich extract from Hibiscus sabdariffa calyx counteracts UVC-caused impairments in rats.

    Science.gov (United States)

    Ozkol, Hatice Uce; Koyuncu, Ismail; Tuluce, Yasin; Dilsiz, Nihat; Soral, Sinan; Ozkol, Halil

    2015-01-01

    Ultraviolet radiation (UV) was reported to cause oxidative stress. Hibiscus sabdariffa L. (Malvaceae) calyx is commonly used in traditional Asian and African medicines and possesses strong antioxidant capacity due to its anthocyanin (ANTH) content. This study researched the possible protective role of Hibiscus sabdariffa calyx extract (HSCE) in UVC exposure of rats. Levels of serum enzymes, renal function tests, and some oxidant/antioxidant biomarkers of skin, lens, and retina tissues were monitored. Rats were exposed to UVC 4 h daily for 40 d and simultaneously received HSCE containing 2.5, 5, and 10 mg doses of ANTH in drinking water. Significant (p < 0.05) increases in the levels of serum aminotransferases, lactate dehydrogenase, urea, creatinine, and uric acid were noted after UVC exposure. In skin, lens, and retina tissues, total oxidant status, oxidative stress index, lipid peroxidation, and protein oxidation escalated markedly (p < 0.05) whereas total antioxidant status, reduced glutathione, and superoxide dismutase decreased dramatically (p < 0.05) related to UVC. Co-administration of HSCE with each ANTH dose significantly (p < 0.05) reversed aforementioned parameters (except total oxidant status) almost in all tissues. The LD50 of HSCE in rats was determined to be above 5000 mg/kg. Our data revealed that HSCE has a remarkable potential to counteract UVC-caused impairments, probably through its antioxidant and free radical-defusing effects. Therefore, HSCE could be useful against some cutaneous and ocular diseases in which UV and oxidative stress have a role in the etiopathogenesis.

  4. Disruption of Angiogenesis by Anthocyanin-Rich Extracts of Hibiscus sabdariffa

    Science.gov (United States)

    Joshua, Madu; Okere, Christiana; Sylvester, O’Donnell; Yahaya, Muhammad; Precious, Omale; Dluya, Thagriki; Um, Ji-Yeon; Neksumi, Musa; Boyd, Jessica; Vincent-Tyndall, Jennifer; Choo, Dong-Won; Gutsaeva, Diana R.; Jahng, Wan Jin

    2017-01-01

    Abnormal vessel formations contribute to the progression of specific angiogenic diseases including age-related macular degeneration. Adequate vessel growth and maintenance represent the coordinated process of endothelial cell proliferation, matrix remodeling, and differentiation. However, the molecular mechanism of the proper balance between angiogenic activators and inhibitors remains elusive. In addition, quantitative analysis of vessel formation has been challenging due to complex angiogenic morphology. We hypothesized that conjugated double bond containing-natural products, including anthocyanin extracts from Hibiscus sabdariffa, may control the proper angiogenesis. The current study was designed to determine whether natural molecules from African plant library modulate angiogenesis. Further, we questioned how the proper balance of anti- or pro-angiogenic signaling can be obtained in the vascular microenvironment by treating anthocyanin or fatty acids using chick chorioallantoic membrane angiogenesis model in ovo. The angiogenic morphology was analyzed systematically by measuring twenty one angiogenic indexes using Angiogenic Analyzer software. Chick chorioallantoic model demonstrated that anthocyanin-rich extracts inhibited angiogenesis in time- and concentration-dependent manner. Molecular modeling analysis proposed that hibiscetin as a component in Hibiscus may bind to the active site of vascular endothelial growth factor receptor 2 (VEGFR2) with ΔG= −8.42 kcal/mol of binding energy. Our results provided the evidence that anthocyanin is an angiogenic modulator that can be used to treat uncontrolled neovascular-related diseases, including age-related macular degeneration. PMID:28459020

  5. Physiological and biochemical responses of Hibiscus sabdariffa to drought stress in the presence of salicylic acid

    Directory of Open Access Journals (Sweden)

    Marzieh Mirshekari

    2017-08-01

    Salicylic acid (SA is one of the important signal molecules, which modulates plant responses to environmental stress. In the present work, impact of exogenous SA on some physiological and biochemical traits of Hibiscus sabdariffa in response to drought stress was studied. Hibiscus sabdariffa seedlings were exposed to six drought levels (0, -0.05, -0.1, -0.5, -0.75, and 1 MPa with two SA concentrations (0 and 500 µM in 5 days intervals up to 20 days in a factorial design. During drought stress period, the root and shoot growth, relative water content, pigments content, non-reducing sugar and starch content was significantly decreased. SA treatment cause prevention of the growth reduction and improvement of relative water content. Protein concentration was roughly unchanged during drought stress with SA, while, reducing sugars accumulates and non-reducing sugars and starch significantly decreases. The results show that exogenous SA application on leaves during drought stress can ameliorate detrimental effects of stress through reducing water loss and accumulating reducing sugars, which cause preserving turgor pressure of the cells.

  6. Disruption of Angiogenesis by Anthocyanin-Rich Extracts of Hibiscus sabdariffa.

    Science.gov (United States)

    Joshua, Madu; Okere, Christiana; Sylvester, O'Donnell; Yahaya, Muhammad; Precious, Omale; Dluya, Thagriki; Um, Ji-Yeon; Neksumi, Musa; Boyd, Jessica; Vincent-Tyndall, Jennifer; Choo, Dong-Won; Gutsaeva, Diana R; Jahng, Wan Jin

    2017-02-01

    Abnormal vessel formations contribute to the progression of specific angiogenic diseases including age-related macular degeneration. Adequate vessel growth and maintenance represent the coordinated process of endothelial cell proliferation, matrix remodeling, and differentiation. However, the molecular mechanism of the proper balance between angiogenic activators and inhibitors remains elusive. In addition, quantitative analysis of vessel formation has been challenging due to complex angiogenic morphology. We hypothesized that conjugated double bond containing-natural products, including anthocyanin extracts from Hibiscus sabdariffa , may control the proper angiogenesis. The current study was designed to determine whether natural molecules from African plant library modulate angiogenesis. Further, we questioned how the proper balance of anti- or pro-angiogenic signaling can be obtained in the vascular microenvironment by treating anthocyanin or fatty acids using chick chorioallantoic membrane angiogenesis model in ovo. The angiogenic morphology was analyzed systematically by measuring twenty one angiogenic indexes using Angiogenic Analyzer software. Chick chorioallantoic model demonstrated that anthocyanin-rich extracts inhibited angiogenesis in time- and concentration-dependent manner. Molecular modeling analysis proposed that hibiscetin as a component in Hibiscus may bind to the active site of vascular endothelial growth factor receptor 2 (VEGFR2) with ΔG= -8.42 kcal/mol of binding energy. Our results provided the evidence that anthocyanin is an angiogenic modulator that can be used to treat uncontrolled neovascular-related diseases, including age-related macular degeneration.

  7. Comparison of leaf anatomical characteristics of hibiscus rosa-sinensis grown in faisalabad region

    International Nuclear Information System (INIS)

    Noman, A.; Ali, Q.; Mehmood, T.; Iftikhar, T.; Mahmeed, M.

    2014-01-01

    The genetic potential of different plant species to different environmental conditions differ in relation to different physiological, biochemical and anatomical characteristics. Of these varying attributes leaf anatomical characteristics play most important role for the establishment of that cultivar in varied environmental conditions. So, the present study was conducted to assess the inter-cultivar genetic potential of Hibiscus in relation to leaf anatomical characteristics. To fulfill the study requirements Hibiscus rosa-sinensis and its six cultivars (were well adapted to their specific natural habitat) were collected from different locations of district Faisalabad Pakistan that have great environmental changes round the year. Results showed significant variability among cultivars in relation to analyzed anatomical characteristics. Cultivars Lemon shiffon and Wilder's white emerge more promising among others by possessing more epidermal thickness, increased epidermal cell area, high cortical cell area and incremented stomatal density as compared with other cultivars. On the other hand, cultivars Cooperi alba, Mrs. George Davis and Frank green possessed least cortex cell area, lowest xylem region thickness and minimum phloem region thickness respectively. Overall, it can be concluded that anatomical genetic potential has endorsed cultivars Lemon chiffon and Wilder's white with enormous capability to grow well under variable environments. (author)

  8. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    Samman Fatima

    2016-01-01

    Full Text Available Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae, is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14% of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83% was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  9. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.

    Directory of Open Access Journals (Sweden)

    Ahmed Mahmood Ibrahim

    2014-01-01

    Full Text Available Kenaf (Hibiscus cannabinus L. is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.

  10. OPTIMISATION OF HIBISCUS SABDARIFFA AS A NATURAL COAGULANT TO TREAT CONGO RED IN WASTEWATER

    Directory of Open Access Journals (Sweden)

    MUN Y. YONG

    2016-02-01

    Full Text Available The process of coagulation is commonly practiced in water and wastewater treatment to reduce level of dissolved chemical, turbidity and so on with the usage of coagulant. Aluminium sulphate (alum is the most commonly used coagulant, however, recent studies show that residual aluminium in drinking water and sludge may induce Alzheimer’s disease and environmental issues. Natural coagulant which is environmental friendly and non-toxic is developed as an alternative to overcome these issues. In this work, Hibiscus Sabdariffa was studied as natural coagulant to treat dye wastewater containing Congo red. The seeds were extracted with different solvent such as distilled water, 0.5 M NaCl and 0.05 M NaOH to extract the coagulation agent. The working parameters were optimised using Response Surface Methodology (RSM. 0.5 M NaCl was found to have highest colour removal of 95.1 % among the solvents. In addition, Hibiscus Sabdariffa seed was found to be an effective coagulant that has 91.2 % colour removal at the optimal working condition of pH 2, 190 mg/L coagulant dosage at 400 ppm of dye concentration. It was also been identified that the performance of natural coagulant is comparable with conventional coagulant, aluminium sulphate with colour removal of 91.2 % and 92.3 % respectively.

  11. INHIBITION OF HUMAN LOW-DENSITY LIPOPROTEINS OXIDATION BY Hibiscus radiatus CUV. CALYCES EXTRACT

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    Hernawan Hernawan

    2010-06-01

    Full Text Available Hibiscus radiatus Cuv calyces extracts rich in polyphenols was screened for their potential to inhibit oxidation of human low-density lipoproteins-cholesterol (LDL-C in vitro. The inhibition of LDL-C oxidation (antioxidant activity was determined by measuring the formation of conjugated dienes and thiobarbituric acid reagent substances (TBARS. LDL-C oxidation was carried out in the presence of H. radiatus Cuv calyces extract (20 and 50 μM. CuSO4 (10 μM was used as the oxidation initiator and  butylated hydroxytoluene (BHT at 50 μM was used as standard antioxidant. The protective effect of H. radiatus Cuv. calyces extract toward human low-density lipoproteins, complex lipid system was  demonstrated by significant increase lag time (> 103 min, diminished of the propagation rate (44 %, and diminution of conjugated dienes formation 59.42 % (50 μM compared to control.   Keywords: antioxidant, conjugated dienes, Hibiscus radiatus Cuv, low-density lipoproteins-cholesterol

  12. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

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    Vanzella Cláudia

    2012-04-01

    Full Text Available Abstract Background Hibiscus tiliaceus L. (Malvaceae is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect.

  13. Effect of Chronic Gamma Irradiation on Kenaf (Hibiscus cannabinus. L) Variety V36

    International Nuclear Information System (INIS)

    Mohd Zulmadi Sani; Faiz Ahmad; Mustapha Akil; Zaiton Ahmad; Affrida Abu Hassan; Abdul Rahim Harun

    2016-01-01

    Kenaf (Hibiscus cannabinus) is originated from Africa and used in making various products such as animal food pallet, clothes, particle board, bio-plastics, paper and many other fibre related products. Due to its commercial value in various industries, research has been carried out to obtain kenaf with improved traits. Mutation breeding using ionizing radiation has been used widely and proven to be effective as an alternative method in plant improvement. In this study, chronic gamma irradiation on kenaf (Hibiscus cannabinus. L) Variety V36 was carried out at Gamma Greenhouse (GGH) facility at Malaysia Nuclear Agency. Kenaf seeds were planted in pots at the shade house and transferred to GGH after 7 days. These seedlings were subjected to various dose rates of 0.3 Gy/ hour, 0.17 Gy/ hour, 0.11 Gy /hour, 0.05 Gy/ hour, 0.03 Gy/ hour, 0.02 Gy/ hour, and 0 Gy/ hour (control). Plant survival and morphological data such as plant height and stem girth, were observed. From the result obtain, at accumulated dose of 230 Gy (Ring 4), plant showed the highest average plant height, stem girth and highest morphological changes. The finding of this study is useful in determining the most suitable dose rate for chronic gamma irradiation in the future. (author)

  14. Hibiscus sabdariffa anthocyanins-rich extract: Chemical stability, in vitro antioxidant and antiproliferative activities.

    Science.gov (United States)

    Maciel, Laércio Galvão; do Carmo, Mariana Araújo Vieira; Azevedo, Luciana; Daguer, Heitor; Molognoni, Luciano; de Almeida, Mereci Mendes; Granato, Daniel; Rosso, Neiva Deliberali

    2018-03-01

    Hibiscus sabdariffa calyx is a rich source of anthocyanins and other bioactive compounds but no study reported the effects of experimental conditions on the extraction of these chemical compounds. Therefore, the effects of time and extraction temperature on the bioactive compounds and antioxidant activity of Hibiscus sabdariffa calyx were evaluated. In addition, the effects of copigmentation and pH on the stability of anthocyanins were assessed and the cytotoxic effects (LC 50 , IC 50 , and GC 50 ) of the extracts were determined in relation to tumor cell lines - Caco-2, HepG-2, HCT8, and A549. The temperature significantly influenced the total anthocyanins and flavonoids contents. The interaction between time/temperature influenced the total phenolic content and ascorbic acid. The t 1/2 and the percentage of colour retention decreased markedly at temperatures above 80 °C. Variations in pH conserved the antioxidant activity of the anthocyanins, and the protonation-deprotonation process of the extract was reversible. The treatment of cells with purified anthocyanin extract or crude extracts at 5-800 μg mL -1 did not show significant cytotoxic effects on the cell lines, corroborating the chemical antioxidant effect of the extracts (DPPH assay). Cyanidin-3-glucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, and cyanidin-3-sambubioside were identified in the extracts by LC-ESI-MS. Copyright © 2018 Elsevier Ltd. All rights reserved.

  15. In vitro antioxidant, anti-inflammatory, cytotoxic activities against prostate cancer of extracts from Hibiscus sabdariffa leaves.

    Science.gov (United States)

    Worawattananutai, Patsorn; Itharat, Arunporn; Ruangnoo, Srisopa

    2014-08-01

    Hibiscus sabdariffa (HS) leaves are a vegetable, which is used as a healthy sour soup for protection against chronic diseases in Thai traditional medicine. To investigate antioxidant, anti-inflammatory and cytotoxic activities of Hibiscus sabdariffa leave extracts from diferent extraction methods. Fresh and dry Hibiscus sabdariffa leaves were extracted by various methods such as maceration with 95% and 50% ethanol, squeeze, and boiling with water or decoction. All extracts were testedfor antioxidant activity by using DPPH radical scavenging assay, anti-inflammatory activity by determination on inhibitory effect of nitric oxide production on RAW264. 7 cell. Cytotoxic activity also tested against human prostate cancer cell line (PC-3) by using sulforhodamine B (SRB) assay. Total phenolic content determined by the Folin-Ciocalteu colorimetric method. The results found that the 95% ethanolic extract of Hibiscus sabdariffa dried leaves (HSDE95) showed the highest antioxidant activity with an EC50 of 34.51±2.62 μg/ml and had the highest phenolic content (57.00±3.73 mg GAE/g). HSDE95 also showed potent cytotoxicity against prostate cancer cell line with an IC50 of 8.58±0.68 μg/ml whereas HSDE95 and all of extracts ofHibiscus sabdariffa leaves had no anti-inflammatory activity. The obtained results revealed that HSDE95 extract showedpotent cytotoxic activity against prostate cancer cells but low antioxidant and anti-inflammatory activities. This extract should be further isolated as active compounds against prostate cancer.

  16. In vitro inhibitory activities of the extract of Hibiscus sabdariffa L. (family Malvaceae) on selected cytochrome P450 isoforms.

    Science.gov (United States)

    Johnson, Showande Segun; Oyelola, Fakeye Titilayo; Ari, Tolonen; Juho, Hokkanen

    2013-01-01

    Literature is scanty on the interaction potential of Hibiscus sabdariffa L., plant extract with other drugs and the affected targets. This study was conducted to investigate the cytochrome P450 (CYP) isoforms that are inhibited by the extract of Hibiscus sabdariffa L. in vitro. The inhibition towards the major drug metabolizing CYP isoforms by the plant extract were estimated in human liver microsomal incubations, by monitoring the CYP-specific model reactions through previously validated N-in-one assay method. The ethanolic extract of Hibiscus sabdariffa showed inhibitory activities against nine selected CYP isoforms: CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4. The concentrations of the extract which produced 50% inhibition of the CYP isoforms ranged from 306 µg/ml to 1660 µg/ml, and the degree of inhibition based on the IC50 values for each CYP isoform was in the following order: CYP1A2 > CYP2C8 > CYP2D6 > CYP2B6 > CYP2E1 > CYP2C19 > CYP3A4 > CYP2C9 > CYP2A6. Ethanolic extract of Hibiscus sabdariffa caused inhibition of CYP isoforms in vitro. These observed inhibitions may not cause clinically significant herb-drug interactions; however, caution may need to be taken in co-administering the water extract of Hibiscus sabdariffa with other drugs until clinical studies are available to further clarify these findings.

  17. Optimasi Kombinasi Aspartam- Tropicana Slim® Sebagaipemanis Dalam Formulasi Tablet Effervescent Dari Ekstrak Kelopak Bunga Rosella (Hibiscus Sabdariffa L.) Dengan Metode Simplex Lattice Design

    OpenAIRE

    Aprilianto, Angga

    2011-01-01

    Flower calyces Rosela (Hibiscus sabdariffa L) is a traditional medicine as anti-hypertension is now widely used in various preparations. This research alms to create effervescent tablets of flower calyces extract Rosela (Hibiscus sabdariffa L) with a stable characteristic of simplex lattice design method. Formula optimization used simplex lattice design because this method does not require much effort and money. Then extracts are tested their physical properties including appearance, viscosit...

  18. OPTIMASI KOMBINASI ASPARTAM- TROPICANA SLIM® SEBAGAIPEMANIS DALAM FORMULASI TABLET EFFERVESCENT DARI EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) DENGAN METODE SIMPLEX LATTICE DESIGN

    OpenAIRE

    Aprilianto, Angga

    2016-01-01

    Flower calyces Rosela (Hibiscus sabdariffa L) is a traditional medicine as anti-hypertension is now widely used in various preparations. This research alms to create effervescent tablets of flower calyces extract Rosela (Hibiscus sabdariffa L) with a stable characteristic of simplex lattice design method. Formula optimization used simplex lattice design because this method does not require much effort and money. Then extracts are tested their physical properties including appearance, viscosit...

  19. Honey as a bioindicator of arsenic contamination due to volcanic and mining activities in Chile

    Directory of Open Access Journals (Sweden)

    José M Bastías

    2013-06-01

    Full Text Available The content of heavy metals in honey is indicative of natural or anthropogenic pollution and has therefore been proposed as a feasible bioindicator for arsenic contamination in different regions of Chile. Total arsenic (t-As and inorganic As (i-As concentrations were determined in 227 samples of honey harvested during the years 2007, 2008, and 2009 in the areas of San Pedro de Atacama, Atacama, Chiloé, and Futaleufú, with the last town located 156 km from the Chaitén Volcano (latest eruption in 2008. These analyses were conducted using an atomic absorption spectrophotometer coupled with a hydride generator. In the honey samples, the concentrations of t-As ranged from 2.2 to 171.9 μg kg-1, and the i-As concentrations ranged from none detected (ND to 24.6 μg kg-1, with the area of San Pedro de Atacama having the highest As concentrations. The samples of honey from Futaleufú showed higher As concentrations after the eruption of the Chaitén Volcano in 2008. This study demonstrates that As pollution in honey may originate from both natural and anthropogenic sources. The results indicate that it is appropriate to use honey as a bioindicator of environmental pollution. In addition, the consumption of the honey studied herein does not pose any health hazards to the consumer due to its As content.

  20. Trace element concentrations and bioindicator responses in tree swallows from northwestern Minnesota

    Science.gov (United States)

    Custer, Christine M.; Custer, T.W.; Warburton, D.; Hoffman, D.J.; Bickham, J.W.; Matson, C.W.

    2006-01-01

    Abstract Extremely high concentrations of cadmium (3.5 ug/g dry wgt.) and elevated concentrations of chromium (>10 ug/g dry wgt.) and mercury (1.6 ug/g dry wgt.) were reported in waterbird tissues at Agassiz National Wildlife Refuge in northwestern Minnesota in 1994. Tree swallows (Tachycineta bicolor) were studied during 1998-2001 at three drainages into the Refuge, two pools on the Refuge, and at a nearby reference location to document whether high levels of contaminants were still present, and if so to quantify the source and severity of the contamination. Trace elements were measured in tree swallow eggs, livers, and diet. Reproductive success and bioindicator responses were monitored. In 2000, water was drawn down on Agassiz Pool, one of the main pools on the Refuge. This presented an opportunity to evaluate the response of trace element concentrations in the diet and tissues of tree swallows after reflooding. High concentrations of trace elements were not detected in swallow tissues, nor were there differences among locations. Less than 20% of swallow samples had detectable concentrations of cadmium or chromium. Mercury concentrations were low and averaged Bioindicator measurements were within the normal ranges as well.

  1. Potential use of the oligochaete Limnodrilus profundicola V., as a bioindicator of contaminant exposure.

    Science.gov (United States)

    Ozdemir, Adile; Duran, Mustafa; Sen, Alaattin

    2011-02-01

    Cholinesterase (ChE) and ethoxyresorufin-O-deethylase (EROD) were of special interest to this study as these biochemical tools have been widely used for the determination of exposure to pollutants. In this study, the freshwater oligochaete Limnodrilus profundicola was tested for its potential as a bioindicator of freshwater pollution. For this purpose, the ChE and EROD activities of L. profundicola and the level of polycyclic aromatic hydrocarbons (PAH) of water samples collected from different sites along the Curuksu stream on the Menderes River (the ancient Meander) running through south-western Turkey were studied. First, these activities were characterized using, as model substrates, acetylthiocholine (ATC), propionylthiocholine (PTC), and butyrylthiocholine (BTC). Then, the in vivo effects of insecticides and pollutants on these activities were investigated. L. profundicola were exposed to various doses of methyl-parathion, methomyl, and deltamethrin. Although significant inhibition of ChE was detected with each of the insecticides, the highest level of inhibition was observed with methyl-parathion. In addition to the inhibition of ChE, the activity of EROD was induced by exposure to oil-contaminated sediments. Thus, although L. profundicola has a reputation for being very resistant to pollution (although it is not insensitive to it), we demonstrated that it may potentially be used as a bioindicator species for contaminant exposure when ChE and EROD are used as biomarkers. Copyright © 2010 Wiley Periodicals, Inc.

  2. Freshwater shrimp (Palaemonetes australis) as a potential bioindicator of crustacean health.

    Science.gov (United States)

    Webb, Diane

    2011-07-01

    Palaemonetes australis is a euryhaline shrimp found in southwestern Australian estuaries. To determine if P. australis is a suitable bioindicator species for monitoring the health of estuarine biota, they were exposed to measured concentrations of the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P) at 0.01, 0.1, or 1 ppm for 14 days under laboratory conditions. At the end of exposure the shrimp were sacrificed for biomarker [ethoxycoumarin O-deethylase (ECOD), 8-oxo-dG concentration, and sorbitol dehydrogenase (SDH) activity] analyses. Gender did not appear to influence biomarker responses of the shrimp in this study. ECOD activity was induced in the treatment groups in a linear fashion from three (0.01 ppm) times to 12 (1 ppm) times the negative controls. 8-oxo-dG concentration was reduced three times in treatment groups below the controls suggesting impaired DNA repair pathways. There was no increase in SDH, signifying hepatopancreatic cell damage had not occurred in any treatment group. The response of P. australis to B[a]P exposure indicates that this crustacean is suitable bioindicator species for both laboratory studies and field monitoring. A combination of ECOD and SDH activities and 8-oxo-dG concentration represent a suitable suite of biomarkers for environmental monitoring of the sublethal effects of organic pollution to crustaceans from an estuarine environment.

  3. Lichen as Bioindicator for Monitoring Environmental Status in Western Himalaya, India

    Directory of Open Access Journals (Sweden)

    Sugam Gupta

    2016-05-01

    Full Text Available The use of bioindicator communities (lichen to assess the environmental status of an area is a well-proved strategy to monitor any habitat without any logistic and instrumental facilities. In the present study, 13 bioindicator communities of lichen have been used to assess the environmental status of holy pilgrimage (Badrinath, western Himalaya, India. Three sites (i.e. Badrinath, Mana & pilgrimage route from Bhimpul to Vasudhara have been comparatively assessed. The results of the study reveals that Badrinath site is less polluted and experiences low degree of anthropogenic disturbances compared to Mana, and pilgrimage route (Bhimpul to Vasudhara. Human settlements, construction of civil works, vehicular emission, and trampling and trekking by tourists are the major threats on these habitats, which ultimately decrease the quality of vegetation and adjacent environment. Controlled vehicular use, promotion of modern way of cooking and managed trekking in these pilgrimage routes could be helpful to combat the decreasing vegetation and environmental quality therein.INTERNATIONAL JOURNAL OF ENVIRONMENTVolume-5, Issue-2, Mar-May 2016 page: 1-15

  4. Phytotoxicity of effluents from swine slaughterhouses using lettuce and cucumber seeds as bioindicators.

    Science.gov (United States)

    Gerber, Michel David; Lucia, Thomaz; Correa, Luciara; Neto, José Eduardo Pereira; Correa, Érico Kunde

    2017-08-15

    This study evaluated the phytotoxic effects of raw and treated effluents from a swine slaughterhouse on cucumber and lettuce seeds and determined correlations among physicochemical characteristics of such effluents and the germination of seeds used as bioindicators. Physicochemical parameters were characterized for both effluents and their phytotoxicity was determined through the germination index (GI), the root length (RL) and the number of germinated seeds (SG) for both plant species. The effluents treatment system was efficient to reduce the concentration of some physicochemical parameters to levels within those recommended by the Brazilian legislation, except for P, ammoniacal N and TKN concentration. Although phytotoxicity of the treated effluent was less in comparison to the raw effluent, the GI for cucumber and lettuce seeds submitted to each of the tested effluents was lower than 80%. Thus, both effluents were phytotoxic for the tested bioindicators (peffluent. The Zn concentration in the treated effluent showed a negative correlation (peffluents from swine slaughterhouses may impair the germination of the evaluated plant species if used for agricultural purposes. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Common buzzards (Buteo buteo) bio-indicators of heavy metals pollution in Sicily (Italy).

    Science.gov (United States)

    Naccari, C; Cristani, M; Cimino, F; Arcoraci, T; Trombetta, D

    2009-04-01

    The aim of this study was to evaluate the accumulation of toxic (As, Cd and Pb) and essential (Cu, Mn and Zn) metals in samples (feathers, liver, kidney, lung, intestine and muscle) of common buzzards (Buteo buteo) from Sicily, used as bio-indicators for monitoring environmental metals pollution. All samples of buzzards were collected at the "Recovery Center of Wild Fauna" of Palermo, through the Zooprophilactic Institute. The quantitative determinations of metals were carried out using an atomic absorbtion spectrophotometer (AAS). The results obtained showed the presence of metals in all samples analyzed. For toxic metals the highest levels of Pb and As were found in liver and those of Cd in kidney; for essential metals Zn levels were higher than Cu and Mn in all tissues analyzed. Significant differences are observed in metal levels between female and male and juvenile and adult bird samples. Highest metal levels found in liver, kidney and muscle can be considered indicative of chronic exposure to metals while the presence of metals in feathers can be consequential to storing and elimination processes. The results obtained suggest that common buzzards (Buteo buteo) may be very useful as bio-indicators for monitoring environmental pollution.

  6. Characteristics of Pelargonium radula as a mercury bioindicator for safety assessment of drinking water.

    Science.gov (United States)

    Majid, N A; Phang, I C; Darnis, D S

    2017-10-01

    Identification of Pelargonium radula as bioindicator for mercury (Hg) detection confers a new hope for monitoring the safety of drinking water consumption. Hg, like other non-essential metals, inflicts the deterioration of biological functions in human and other creatures. In the present study, effects of Hg on the physiology and biochemical content of P. radula were undertaken to understand the occurrence of the morphological changes observed. Young leaves of P. radula were treated with different concentrations of Hg-containing solution (0.5, 1.0 and 2.0 ppb) along with controls for 4 h, prior to further analysis. Elevated Hg concentration in treatment solution significantly prompted an increased accumulation of Hg in the leaf tissues. Meanwhile, total protein, chlorophyll and low molecular mass thiol contents (cysteine, glutathione and oxidized glutathione) decreased as Hg accumulation increased. However, phytochelatin 2 productions were induced in the treated leaves, in comparison to the control. Based on these findings, it is postulated that as low as 0.5 ppb of Hg interferes with the metabolic processes of plant cells, which was reflected from the morphological changes exhibited on P. radula leaves-the colour of the Hg-treated leaves changed from green to yellowish-brown, became chlorosis and wilted. Changes in the tested characteristics of plant are closely related to the Hg-induced morphological changes on P. radula leaves, a potential bioindicator for detecting Hg in drinking water.

  7. Lichen elemental content bioindicators for air quality in upper Midwest, USA: A model for large-scale monitoring

    Science.gov (United States)

    Susan Will-Wolf; Sarah Jovan; Michael C. Amacher

    2017-01-01

    Our development of lichen elemental bioindicators for a United States of America (USA) national monitoring program is a useful model for other large-scale programs. Concentrations of 20 elements were measured, validated, and analyzed for 203 samples of five common lichen species. Collections were made by trained non-specialists near 75 permanent plots and an expert...

  8. Gomphrena claussenii, a novel metal-hypertolerant bioindicator species, sequesters cadmium, but not zinc, in vacuolar oxalate crystals

    NARCIS (Netherlands)

    Villafort Carvalho, M.T.; Pongrac, P.; Mumm, R.; Arkel, van J.; Aelst, van A.; Jeromel, L.; Vavpetic, P.; Pelicon, P.; Aarts, M.G.M.

    2015-01-01

    Gomphrena claussenii is a recently described zinc (Zn)- and cadmium (Cd)-hypertolerant Amaranthaceae species displaying a metal bioindicator Zn/Cd accumulation response. We investigated the Zn and Cd distribution in stem and leaf tissues of G. claussenii at the cellular level, and determined

  9. Dendritic Cell-Mediated T Cell Proliferation -A Functional Bioindicator of Inflammatory Source-Specific Particulate Matter

    Science.gov (United States)

    Previously we found that dendritic cells (DC) were sensitive functional bioindicators of ambient PM (APM) exposure mediating Th2-allergic inflammation in the draining lymph nodes. Here, the ability of bone-marrow-derived DC (DC) and putative BM-derived basophils (Ba) to present a...

  10. Field assessment of a snap bean ozone bioindicator system under elevated ozone and carbon dioxide in a free air system

    Science.gov (United States)

    Ozone-sensitive (S156) and -tolerant (R123 and R331) genotypes of snap bean (Phaseolus vulgaris L.) were tested as a plant bioindicator system for detecting O3 effects at current and future levels of tropospheric O3 and atmospheric CO2 under field conditions. Plants were exposed to reciprocal combi...

  11. Bio-indicator species and Central African rain forest refuges in the Campo-Ma'an area, Cameroon

    NARCIS (Netherlands)

    Tchouto, M.G.P.; de Wilde, J.J.F.E.; de Boer, W.F.; van der Maesen, L.J.G.; Cleef, A.M.

    2009-01-01

    This study aims to examine the geographical position of late Pleistocene forest refuges in the tropical lowland rain forest in southern Cameroon by analysing the distribution of 178 selected bio-indicator species. We studied the distribution patterns of these species, such as strict and narrow

  12. Termites community as environmental bioindicators in highlands: a case study in eastern slopes of Mount Slamet, Central Java

    Directory of Open Access Journals (Sweden)

    IDHAM SAKTI HARAHAP

    2011-10-01

    Full Text Available Pribadi T,Raffiudin R,HarahapIS (2011Termites community as environmental bioindicators in highlands: a case study in eastern slopes of Mount Slamet, Central Java. Biodiversitas 12: 235-240. Termites ecological behaviour is much affected by land use change and disturbance level. Their variation in diversity can be used as bioindicator of environmental quality. However, termite community response to land use changes and habitat disturbance in highland ecosystems remains poorly understood. This study was conducted to investigate the response of termite community to land use intensification and to explore their role as environmental bioindicator in Mount Slamet. A standard survey protocol was used to collect termites in five land use typesof various disturbance levels,i.e. protected forest, recreation forest, production forest,agroforestry, and urban area. It was found two termite families i.e. Rhinotermitidae and Termitidae with seven species, i.e Schedorhinotermes javanicus, Procapritermes sp, Pericapritermes semarangi, Macrotermes gilvus, Microtermes insperatus, Nasutitermes javanicus, and N. matanganensis. Termite species’ richness and evenness, Shannon-Wiener index, relative abundance, and biomass of termite were declined along with the land use types and disturbance level from protected forest to urban area. Habitat disturbance was the main declining factor of termite diversity. Termite composition changed along with the land use disturbance level. Soil feeding termites were sensitive to the disturbance – they were not found in urban area. Hence, their presence or absence can be used as environmental bioindicator to detect habitat disturbance.

  13. Variability of cirrus clouds in a convective outflow during the Hibiscus campaign

    Science.gov (United States)

    Fierli, F.; di Donfrancesco, G.; Cairo, F.; Marécal, V.; Zampieri, M.; Orlandi, E.; Durry, G.

    2008-08-01

    Light-weight microlidar and water vapour measurements were taken on-board a stratospheric balloon during the HIBISCUS 2004 campaign, held in Bauru, Brazil (49° W, 22° S). Cirrus clouds were observed throughout the flight between 12 and 15 km height with a high mesoscale variability in optical and microphysical properties. It was found that the cirrus clouds were composed of different layers characterized by marked differences in height, thickness and optical properties. Simultaneous water vapour observations show that the different layers are characterized by different values of the saturation with respect to ice. A mesoscale simulation and a trajectory analysis clearly revealed that the clouds had formed in the outflow of a large and persistent convective region and that the observed variability of the optical properties and of the cloud structure is likely linked to the different residence times of the convectively-processed air in the upper troposphere.

  14. A Stylistic Analysis of Linguistic Patterns in Chichamanda Ngozi Adichie’s Purple Hibiscus

    Directory of Open Access Journals (Sweden)

    Muchamad Sholakhuddin Al Fajri

    2017-06-01

    Full Text Available This study aims to carry out a detailed and systematic stylistic analysis of linguistic patterns in Purple Hibiscus Novel by Chichamanda Ngozi Adichie. It particularly analyses a specific extract of the novel in terms of narration and point of view, conversational analysis, speech and thought presentations and mind style, and how these linguistic devices and patterns are employed by the author to shape characters’ personalities and relationships between them in the reader’s mind. The result appears to suggest that the author successfully represents the protagonist, Kambili as an obedient and a salient daughter who respects deeply his father, while her father, Eugene, is constructed as a strict father and religious who imposes an absolute control on his daughter.

  15. Hibiscus sabdariffa L extract drying with different carrier agent: Drying rate evaluation and color analysis

    Science.gov (United States)

    Djaeni, M.; Utari, F. D.; Kumoro, A. C.

    2017-03-01

    The aim of this study was to investigate the effect of different carrier agents on roselle or Hibiscus sabdariffa L.extract drying. Carrier agent was used for reducing stickiness of material and avoiding agglomeration as well as improving stability. The method consisted of two steps involving roselle extraction and drying process. The liquid roselle extract was mixed with carrier agent (maltodextrin-arabic gum) in various composition. The mixture was then dried at different air temperature ranging 40 - 80°C. As a response, moisture content in the extract was observed by gravimetry every 10 minutes during90 minutes. The procedurewas repeated for the drying without carrieragent. The result showed that constant rate of drying with carrier agent was higher up to l.7 times than that of without carrier agent. Based on the color analysis,roselle extract drying with carrier agent also showed reasonable quality.

  16. Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity

    Science.gov (United States)

    Bindhu, M. R.; Umadevi, M.

    2013-01-01

    Synthesis of silver nanoparticles using leaf extract of Hibiscus cannabinus has been investigated. The influences of different concentration of H. cannabinus leaf extract, different metal ion concentration and different reaction time on the above cases on the synthesis of nanoparticles were evaluated. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). The prepared silver nanoparticles were monodispersed, spherical in shape with the average particle size of 9 nm and shows surface plasmon peak at 446 nm. The study also reveals that the ascorbic acid present in H. cannabinus leaf extract has been used as reducing agent. The prepared silver nanoparticle shows good antimicrobial activity against Escherichia coli, Proteus mirabilis and Shigella flexneri.

  17. Flower color changes in three Japanese hibiscus species: further quantitative variation of anthocyanin and flavonols.

    Science.gov (United States)

    Shimokawa, Satoshi; Iwashina, Tsukasa; Murakami, Noriaki

    2015-03-01

    One anthocyanin and four flavonols were detected from the petals of Hibiscus hamabo, H. tiliaceus and H. glaber. They were identified as cyanidin 3-0- sambubioside, gossypetin 3-O-glucuronide-8-O-glucoside, quercetin 7-O-rutinoside, gossypetin 3-O-glucoside and gossypetin 8-O-glucuronide by UV spectra, LC-MS, acid hydrolysis and HPLC. The flavonoid composition was essentially the same among the petals ofH. hamabo, H. tiliaceus and H. glaber, and there was little quantitative variation, except for cyanidin 3-O-sambubioside, the content of which in the petals ofH. tiliaceus and H. glaber was much higher than in that of H. hamabo. Flower colors of H. tiliaceus and H. glaber change from yellow to red, and that of H. hamabo changes from yellow to orange. These changes were caused by contents of anthocyanin and flavonols, which increased after flowering of H. hamabo, H. tiliaceus and H. glaber.

  18. Nonlinear refractive index measurements and self-action effects in Roselle-Hibiscus Sabdariffa solutions

    Science.gov (United States)

    Henari, F. Z.; Al-Saie, A.

    2006-12-01

    We report the observation of self-action phenomena, such as self-focusing, self-defocusing, self-phase modulation and beam fanning in Roselle-Hibiscus Sabdariffa solutions. This material is found to be a new type of natural nonlinear media, and the nonlinear reflective index coefficient has been determined using a Z-scan technique and by measuring the critical power for the self-trapping effect. Z-scan measurements show that this material has a large negative nonlinear refractive index, n 2 = 1 × 10-4 esu. A comparison between the experimental n 2 values and the calculated thermal value for n 2 suggests that the major contribution to nonlinear response is of thermal origin.

  19. Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury.

    Science.gov (United States)

    Bakhtiari, Elham; Hosseini, Azar; Mousavi, Seyed Hadi

    2015-01-01

    Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdariffa exert pharmacological actions such as potent antioxidant. Therefore, in this study we investigated the protective effect of extract of H. sabdariffa against SGD-induced PC12 cells injury. Cells were pretreated with different concentrations of H. sabdariffa extract (HSE) for 2 hr, and then exposed to SGD condition for 6, 12 and 18 hr. SGD caused a major reduction in cell viability after 6, 12, and 18 hr as compared with control cells (psabdariffa has the potential to be used as a new therapeutic approach for neurodegenerative disorders.

  20. Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine.

    Science.gov (United States)

    Abdallah, Emad Mohamed

    2016-01-01

    Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine. The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods. The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect. The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents.

  1. Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats

    Science.gov (United States)

    Idris, Muhd Hanis Md; Budin, Siti Balkis; Osman, Mohamad; Mohamed, Jamaludin

    2012-01-01

    Diabetes mellitus contributes to male sexual dysfunction and infertility by modulating oxidative damage. To date, a number of studies have demonstrated antioxidant properties of Hibiscus sabdariffa Linn. This study was designed to investigate the effects of H. sabdariffa UKMR-2 variety on sperm functioning of streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were allotted into four groups, namely control group (C), H. sabdariffa extract (HSE) group, diabetes group (D) and diabetes plus HSE group (D+HSE). HSE (100 mg/ kg/body weight) was administered orally for 28 consecutive days. After 28-days of supplementation, the rats were sacrificed to obtain epididymal sperm. Administration of HSE significantly lowered the level of fasting blood glucose and increased plasma insulin level in D+HSE group as compared to D group (psabdariffa UKMR-2 variety has a potential protective role against diabetes-induced sperm damage. PMID:27847454

  2. Chemical, morphology and thermal evaluation of cellulose microfibers obtained from Hibiscus sabdariffa.

    Science.gov (United States)

    Sonia, A; Priya Dasan, K

    2013-01-30

    Cellulose is the most abundant biomass material in nature and finds a range of application. Cellulose microfibers were extracted from Hibiscus sabdariffa fibers by steam explosion technique. Structural and surface analysis of the microfibers showed a reduction in diameter and changes in surface morphology from that of the raw fibers. The chemical composition of fibers were analyzed according to the TAPPI standards and showed increase in α-cellulose content and decrease in lignin and hemicelluloses for the microfibers. This has been further confirmed by SEM, XRD and FTIR results. Thermal characterization showed enhanced thermal stability of celluloses microfibers compared to raw fibers. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  3. The effects of sour tea (Hibiscus sabdariffa) on hypertension in patients with type II diabetes.

    Science.gov (United States)

    Mozaffari-Khosravi, H; Jalali-Khanabadi, B-A; Afkhami-Ardekani, M; Fatehi, F; Noori-Shadkam, M

    2009-01-01

    To compare the antihypertensive effectiveness of sour tea (ST; Hibiscus sabdariffa) with black tea (BT) infusion in diabetic patients, this double-blind randomized controlled trial was carried out. Sixty diabetic patients with mild hypertension, without taking antihypertensive or antihyperlipidaemic medicines, were recruited in the study. The patients were randomly allocated to the ST and BT groups and instructed to drink ST and BT infusions two times a day for 1 month. Their blood pressure (BP) was measured on days 0, 15 and 30 of the study. The mean of systolic BP (SBP) in the ST group decreased from 134.4+/-11.8 mm Hg at the beginning of the study to 112.7+/-5.7 mm Hg after 1 month (P-value hypertension. This study supports the results of similar studies in which antihypertensive effects have been shown for ST.

  4. Antihypertensive effect of an aqueous extract of the calyx of Hibiscus sabdariffa.

    Science.gov (United States)

    Mojiminiyi, F B O; Dikko, M; Muhammad, B Y; Ojobor, P D; Ajagbonna, O P; Okolo, R U; Igbokwe, U V; Mojiminiyi, U E; Fagbemi, M A; Bello, S O; Anga, T J

    2007-06-01

    The present study was designed to investigate the efficacy of an aqueous calyx extract of Hibiscus sabdariffa (HS) in two forms of experimental hypertension: salt-induced and L-NAME (N(omega)-L-arginine methyl ester)-induced and in normotensive controls. The blood pressure and heart rate fell dose-dependently in both the hypertensive and normotensive rats after intravenous injection of 1-125 mg/kg of HS, suggesting that HS possesses anti-hypertensive, hypotensive and negative chronotropic effects. The fall in mean arterial pressure was significantly pronounced in the hypertensive rats (salt-induced: 94.4+/-8.6 mm Hg; L-NAME-induced: 136.5+/-10.3 mm Hg) than in the normotensive controls (50.2+/-5.1 mm Hg; P<0.05).

  5. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L. Seeds

    Directory of Open Access Journals (Sweden)

    Nurul Hanisah Juhari

    2018-02-01

    Full Text Available Milled Roselle (Hibiscus sabdariffa L. seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air (LO, light/nitrogen (LN, darkness/oxygen (air (DO, and darkness/nitrogen (DN while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  6. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L.) Seeds.

    Science.gov (United States)

    Juhari, Nurul Hanisah; Petersen, Mikael Agerlin

    2018-02-11

    Milled Roselle ( Hibiscus sabdariffa L.) seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air) (LO), light/nitrogen (LN), darkness/oxygen (air) (DO), and darkness/nitrogen (DN) while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  7. OPTIMIZATION OF HIBISCUS SABDARIFFA L. (ROSELLE ANTHOCYANIN AQUEOUS-ETHANOL EXTRACTION PARAMETERS USING RESPONSE SURFACE METHODOLOGY

    Directory of Open Access Journals (Sweden)

    ANILÚ MIRANDA-MEDINA

    2018-03-01

    Full Text Available Anthocyanins along with protocatechuic acid and quercetin have been recognized as bioactive compounds in Hibiscus sabdariffa L. aqueous extracts. Characteristic anthocyanin absorption in the visible region makes their quantification possible without the interference of the other two compounds, and also can favor its potential application as an alternative to organic-based dye sensitized solar cell, in various forms. In order to optimize measurable factors linked to the extraction of these flavonoids, an optimization was performed using a Box-Behnken experimental design and response surface methodology (RSM. Three levels of ethanol concentration, temperature and solid-solvent ratio (SSR were investigated. The optimization model showed that with 96 % EtOH, 65 °C, and 1:50 SSR, the highest anthocyanin concentration of 150 mg/100 g was obtained.

  8. Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts.

    Science.gov (United States)

    Nayak, Debasis; Ashe, Sarbani; Rauta, Pradipta Ranjan; Nayak, Bismita

    2015-10-01

    Green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. In this study, silver nanoparticles (AgNPs) were synthesised using petal extracts of Hibiscus rosa-sinensis. The AgNPs displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mV. The X-ray diffraction patterns displayed typical peaks of face centred cubic crystalline silver. The surface morphology was characterised by scanning electron microscopy and atomic force microscopy. Fourier transform infrared spectroscopy studies confirmed the surface modifications of the functional groups for the synthesis of AgNPs. Furthermore, the synthesised AgNPs displayed proficient antimicrobial activity against pathogenic strains of Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus.

  9. Concurrent analysis of bioactive triterpenes oleanolic acid and β-amyrin in antioxidant active fractions of Hibiscus calyphyllus, Hibiscus deflersii and Hibiscus micranthus grown in Saudi Arabia by applying validated HPTLC method

    Directory of Open Access Journals (Sweden)

    Nasir A. Siddiqui

    2018-02-01

    Full Text Available In this study, we developed a validated HPTLC method for concurrent analysis of two natural antioxidant triterpenes, oleanolic acid (OA and β-amyrin (BA in the biologically active fractions (petroleum ether, toluene, chloroform, ethyl acetate and n-butanol of aerial parts of three Hibiscus species (H. calyphyllus, H. deflersii and H. micranthus. The chromatography was conducted on normal HPTLC (ready to use glass-plate coated with silica gel 60 F254 plate with chloroform and methanol (97:3, V/V used as mobile phase. The derivatization of the developed plate was done with p-anisaldehyde and scanned at λmax = 575 nm. Well resolved and intense peaks of OA and BA were obtained at Rf = 0.36 and 0.57, respectively. The linear regression equation/correlation coefficient (r2 for OA and BA were Y = 6.65x + 553.35/0.994 and Y = 9.177x + 637.23/0.998, respectively in the linearity range of 100–1200 ng/spot indicated good linear relationship. The low values of %RSD for intra-day/inter-day precision of OA (1.45–1.61/1.38–1.59 and BA (1.52–1.57/1.50–1.53 suggested that the method was precise. The recovery/RSD (% values for OA and BA were found to be 99.21–99.62/1.39–1.95 and 98.75–99.70/1.56–1.80, respectively assures the reasonably good accuracy of the proposed method. Fifteen samples were analyzed to check the content of OA and BA by using the developed HPTLC methods. The content of OA in different samples were found to be 3.87 (HmP > 1.212 (HcP > 0.673 (HdC > 0.493 (HdP > 0.168 (HdE > 0.059 (HcC > 0.015 (HcE > 0.008 (HmT µg/mg of the dried weight of extract. However the content of BA was found as: 2.293 (HmP > 1.852 (HdT > 0.345 (HdC > 0.172 (HmT > 0.041 (HdE > 0.008 (HcC µg/mg of the dried weight of extract. Some Hibiscus species fractions exhibited good antioxidant potential like: HcE (IC50 = 17.6 ± 1.8 > HdB (IC50 = 32.16 ± 0.9

  10. Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cells

    International Nuclear Information System (INIS)

    Chang, Y.-C.; Huang, H.-P.; Hsu, J.-D.; Yang, S.-F.; Wang, C.-J.

    2005-01-01

    Hibiscus sabdariffa Linne (Malvaceae), an attractive plant believed to be native to Africa, is cultivated in the Sudan and Eastern Taiwan. Anthocyanins exist widely in many vegetables and fruits. Some reports demonstrated that anthocyanins extracted from H. sabdariffa L., Hibiscus anthocyanins (HAs) (which are a group of natural pigments existing in the dried calyx of H. sabdariffa L.) exhibited antioxidant activity and liver protection. Therefore, in this study, we explored the effect of HAs on human cancer cells. The result showed that HAs could cause cancer cell apoptosis, especially in HL-60 cells. Using flow cytometry, we found that HAs treatment (0-4 mg/ml) markedly induced apoptosis in HL-60 cells in a dose- and time-dependent manner. The result also revealed increased phosphorylation in p38 and c-Jun, cytochrome c release, and expression of tBid, Fas, and FasL in the HAs-treated HL-60 cells. We further used SB203580 (p38 inhibitor), PD98059 (MEK inhibitor), SP600125 (JNK inhibitor), and wortmannin (phosphatidylinositol 3-kinase; PI-3K inhibitor) to evaluate their effect on the HAs-induced HL-60 death. The data showed that only SB203580 had strong potential in inhibiting HL-60 cell apoptosis and related protein expression and phosphorylation. Therefore, we suggested that HAs mediated HL-60 apoptosis via the p38-FasL and Bid pathway. According to these results, HAs could be developed as chemopreventive agents. However, further investigations into the specificity and mechanism(s) of HAs are needed

  11. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    Directory of Open Access Journals (Sweden)

    M. Kaleemullah

    2017-07-01

    Full Text Available Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor (f2 value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05 between the F3 and reference drug in terms of MDT and

  12. Physiological responses and tolerance of kenaf (Hibiscus cannabinus L.) exposed to chromium.

    Science.gov (United States)

    Ding, Han; Wang, Guodong; Lou, Lili; Lv, Jinyin

    2016-11-01

    Selection of kenaf species with chromium (Cr) tolerance and exploring the physiological mechanisms involved in Cr tolerance are crucial for application of these species to phyto-remediation. In the present study, a hydroponic experiment was conducted to investigate the variation in two kenaf cultivars, K39-2 and Zhe50-3 under Cr stress. At the same Cr concentration, the tolerance index (TI) of K39-2 was higher than that of Zhe50-3, indicating that K39-2 may be more tolerant to Cr than Zhe50-3. It was also observed that high concentration of chromium was accumulated both in the shoots and the roots of Hibiscus cannabinus L. The leaves of K39-2 accumulated 4760.28mgkg(-1) of dry weight under 1.50mM Cr stress, and the roots accumulated 11,958.33mgkg(-1). Physiological response shows that the antioxidant enzymes' superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities increased in the leaves and decreased in roots of the Cr-stressed plants nearly compared to the control. Moreover, the variation of antioxidant enzymes activities indicated Zhe50-3 was more vulnerable than K39-2, and the contents of the non-protein thiol pool (GSH, NPT and PCs) were higher in K39-2 than Zhe50-3 with the increased Cr concentration. Based on the observations above, it can be concluded that the well-coordinated physiological changes confer a greater Cr tolerance to K39-2 than Zhe50-3 under Cr exposure, and Hibiscus cannabinus L. has a great accumulation capacity for chromium. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Science.gov (United States)

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina; Volpato, Gustavo Tadeu

    2017-01-01

    The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  14. Plant growth retardation and conserved miRNAs are correlated to Hibiscus chlorotic ringspot virus infection.

    Science.gov (United States)

    Gao, Ruimin; Wan, Zi Yi; Wong, Sek-Man

    2013-01-01

    Virus infection may cause a multiplicity of symptoms in their host including discoloration, distortion and growth retardation. Hibiscus chlorotic ringspot virus (HCRSV) infection was studied using kenaf (Hibiscus cannabinus L.), a non-wood fiber-producing crop in this study. Infection by HCRSV reduced the fiber yield and concomitant economic value of kenaf. We investigated kenaf growth retardation and fluctuations of four selected miRNAs after HCRSV infection. Vegetative growth (including plant height, leaf size and root development) was severely retarded. From the transverse and radial sections of the mock and HCRSV-infected kenaf stem, the vascular bundles of HCRSV-infected plants were severely disrupted. In addition, four conserved plant developmental and defence related microRNAs (miRNAs) (miR165, miR167, miR168 and miR171) and their respective target genes phabulosa (PHB), auxin response factor 8 (ARF8), argonaute 1 (AGO1) and scarecrow-like protein 1 (SCL1) displayed variation in expression levels after HCRSV infection. Compared with the mock inoculated kenaf plants, miR171 and miR168 and their targets SCL1 and AGO1 showed greater fluctuations after HCRSV infection. As HCRSV upregulates plant SO transcript in kenaf and upregulated AGO1 in HCRSV-infected plants, the expression level of AGO1 transcript was further investigated under sulfite oxidase (SO) overexpression or silencing condition. Interestingly, the four selected miRNAs were also up- or down-regulated upon overexpression or silencing of SO. Plant growth retardation and fluctuation of four conserved miRNAs are correlated to HCRSV infection.

  15. Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers.

    Science.gov (United States)

    Joshi, Snehal S; Dice, Lezlee; D'Souza, Doris H

    2015-12-01

    Hibiscus sabdariffa extract is known to have antioxidant, anti-diabetic, and antimicrobial properties. However, their effects against foodborne viruses are currently unknown. The objective of this study was to determine the antiviral effects of aqueous extracts of H. sabdariffa against human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) and hepatitis A virus (HAV) at 37 °C over 24 h. Individual viruses (~5 log PFU/ml) were incubated with 40 or 100 mg/ml of aqueous hibiscus extract (HE; pH 3.6), protocatechuic acid (PCA; 3 or 6 mg/ml, pH 3.6), ferulic acid (FA; 0.5 or 1 mg/ml; pH 4.0), malic acid (10 mM; pH 3.0), or phosphate buffered saline (pH 7.2 as control) at 37 °C over 24 h. Each treatment was replicated thrice and plaque assayed in duplicate. FCV-F9 titers were reduced to undetectable levels after 15 min with both 40 and 100 mg/ml HE. MNV-1 was reduced by 1.77 ± 0.10 and 1.88 ± 0.12 log PFU/ml after 6 h with 40 and 100 mg/ml HE, respectively, and to undetectable levels after 24 h by both concentrations. HAV was reduced to undetectable levels by both HE concentrations after 24 h. PCA at 3 mg/ml reduced FCV-F9 titers to undetectable levels after 6 h, MNV-1 by 0.53 ± 0.01 log PFU/ml after 6 h, and caused no significant change in HAV titers. FA reduced FCV-F9 to undetectable levels after 3 h and MNV-1 and HAV after 24 h. Transmission electron microscopy showed no conclusive results. The findings suggest that H. sabdariffa extracts have potential to prevent foodborne viral transmission.

  16. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Directory of Open Access Journals (Sweden)

    Luana Alves Freitas Afiune

    Full Text Available The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes.Diabetes was induced by streptozotocin (STZ, 40 mg/kg in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group: non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed.The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI and coronary artery risk index (CRI, and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group.Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  17. Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus

    International Nuclear Information System (INIS)

    Cheng, Ao; Speir, Jeffrey A.; Yuan, Y. Adam; Johnson, John E.; Wong, Sek-Man

    2009-01-01

    Hibiscus chlorotic ringspot virus is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family. Authentic virus harvested from infected host kenaf leaves was purified and virus crystals were grown in multiple conditions. One of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 Å. Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV

  18. ROSELLE FLOWER (HIBISCUS SABDARIFFA IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

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    Nikmah Jalilah Ritonga

    2017-07-01

    Full Text Available Background: Roselle flower (Hibiscus sabdariffa has been used as a food and herbal drinks, in hot and cold beverages, as a herbal medicine. Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period. Methods: This was a quasi-experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited using consecutive sampling with 15 samples in the intervention group and control group. Data were analyzed using paired t-test to know the difference of blood pressure value before and after intervention in the intervention and control group, and Independent t-test to determine the difference of blood pressure decrease between intervention and control group. Mann-whitney and Wilcoxon test were also performed for data analysis. Results: Findings showed that there were statistically significant differences in systolic and diastolic blood pressure values before and after intervention in the intervention and control group with p-value 0.000 <0.05 with systolic blood pressure decrease was 5 mmHg and diastolic was 5.33 mmHg. Additionally, there was a significant effect in the duration of healing in the intervention group with p-value 0.000 <0.05, with the average duration of healing was 2 days. Conclusion: There was significant effects of dried petals of rosella flowers (Hibiscus sabdariffa brewed with 200 ml of hot water with temperature 90oC in lowering blood pressure, and there was significant differences in systolic and diastolic blood pressure and duration of healing between the intervention and control group of mothers who consumed antihypertensive drugs. The results of this study can be applied by midwives in postpartum care with the cases of hypertension. It is expected that this intervention can help the healing process more quickly through a combination of antihypertensive medicines and sedated petals of rosella flowers.

  19. ROSELLE FLOWER (HIBISCUS SABDARIFFA IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

    Directory of Open Access Journals (Sweden)

    Nikmah Jalilah Ritonga

    2017-07-01

    Full Text Available Background: Roselle flower (Hibiscus sabdariffa has been used as a food and herbal drinks, in hot and cold beverages, as a herbal medicine. Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period. Methods: This was a Quasi experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited by consecutive sampling with 15 samples in the intervention and control group. Data were analyzed using paired t-test to know the difference of blood pressure value before and after intervention in the intervention and control group, and Independent t-test to determine the difference of blood pressure decrease between intervention and control group. Mann-whitney and Wilcoxon test were also performed for non-normal data distribution. Results: Findings showed that there were statistically significant differences in systolic and diastolic blood pressure values before and after intervention in the intervention group with p-value 0.000 <0.05 with mean of systolic blood pressure decrease was 39.67 mmHg and diastolic was 22.33 mmHg. Additionally, there was a significant difference in the duration of healing between both groups with p-value 0.000 <0.05, with the average duration of healing in the intervention group was 2 days while in the control group was 3-4 days. Conclusion: There was significant effects of dried petals of rosella flowers (Hibiscus sabdariffa in lowering blood pressure, and there was significant differences in systolic and diastolic blood pressure and duration of healing between the intervention and control group of mothers who consumed antihypertensive drugs. The results of this study can be applied by midwives or health services in postpartum care with the cases of hypertension. It is expected that this intervention can help the healing process more quickly through a combination of antihypertensive medicines and

  20. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage.

    Science.gov (United States)

    Kaleemullah, M; Jiyauddin, K; Thiban, E; Rasha, S; Al-Dhalli, S; Budiasih, S; Gamal, O E; Fadli, A; Eddy, Y

    2017-07-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor ( f 2 ) value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f 2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05) between the F3 and reference drug in terms of MDT and T50% with p

  1. Herbal products containing Hibiscus sabdariffa L., Crataegus spp., and Panax spp.: Labeling and safety concerns.

    Science.gov (United States)

    Nunes, Maria Antónia; Rodrigues, Francisca; Alves, Rita C; Oliveira, Maria Beatriz P P

    2017-10-01

    Herbs have been used from ancient times for infusion preparation based on their potential health effects. In particular, the consumption of Hibiscus sabdariffa L., Crataegus spp. and Panax spp. has been largely associated to cardiovascular benefits. In this work, the label information of 52 herbal products for infusion preparation containing the referred herbs was analyzed and discussed, taking into consideration the European Union regulation for herbal products, which intends to protect public health and harmonize the legal framework in Member States. Details about the cardiovascular-related statements and warning notifications about consumption were considered. Also, regulatory issues and possible herb-drug interactions were explored and discussed. A total of 14 of the 52 herbal products selected presented health claims/statements on the label. Hibiscus was present in the majority of the products and, in some cases, it was mentioned only in the ingredients list and not on the product front-of-pack. Despite the promising outcomes of these plants to modulate cardiovascular risk markers, consumers with some sort of cardiovascular dysfunction and/or under medication treatments should be aware to carefully analyze the labels and consult additional information related to these herbal products. Manufacturers have also a huge responsibility to inform consumers by presenting awareness statements. Lastly, health professionals must advise and alert their patients about possible interactions that could occur between the concomitant consumption of drugs and herbs. Overall, there is still a real need of additional studies and clinical trials to better understand herbs effects and establish a science-based guidance to assess their safety. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Antioxidant and DNA damage protective properties of anthocyanin-rich extracts from Hibiscus and Ocimum: a comparative study.

    Science.gov (United States)

    Sarkar, Biswatrish; Kumar, Dhananjay; Sasmal, Dinakar; Mukhopadhyay, Kunal

    2014-01-01

    Anthocyanin extracts (AEs) from Ocimum tenuiflorum (leaf), Hibiscus rosa-sinensis (petal) and Hibiscus sabdariffa (calyx) were investigated and compared for in vitro antioxidant activity and DNA damage protective property. Total phenolic content (TPC) and total anthocyanin content (TAC) of the AEs were determined and the major anthocyanins were characterised. In vitro antioxidant activities were assessed by ferric-reducing antioxidant power (FRAP) assay, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical-scavenging activity, 2-deoxy-D-ribose degradation assay and lipid peroxidation assay. The protective property of the AEs was also examined against oxidative DNA damage by H2O2 and UV using pUC19 plasmid. All the AEs particularly those from O. tenuiflorum demonstrated efficient antioxidant activity and protected DNA from damage. Strong correlation between antioxidant capacity and TPC and TAC was observed. Significant correlation between antioxidant capacity and TPC and TAC ascertained that phenolics and anthocyanins were the major contributors of antioxidant activity.

  3. ffect of Nitrogen and Zinc Foliar Application on Quantitative Traits of Tea Rosslle (Hibiscus sabdariffa in Jiroft Zone

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    abdolreza raisi sarbijan

    2017-02-01

    Full Text Available Introduction: Nitrogen is an essential element forplants and in combination withelements such as carbon, oxygen, hydrogen and sulfur results ineven more valuable materials such as amino acids, nucleic acids, alkaloids. Hibiscus tea (Hibiscus sabdariffa from Malvaceaefamily is known by different names in different parts of the world. In Iran it is calledthe Maki tea, tea Meccaorred tea.As an important plant,it is decided to investigate its growth and development in Jiroft. Materials and Methods The experiment was conducted as factorial based on randomized complete block design with three replications in farm research of Islamic Azad University of Jiroft during 2010. The first factor was nitrogen foliar application in four levels (0, 1, 2 and 3 percent and second factor was foliar application of zinc at twolevels (0 and 1 percent. The measured quantitative characteristics were stem diameter, plant height, calycle fresh weight,calycle dry weight, plant fresh weight,plant dry weight, leaf fresh weight,leaf dry weight, mucilage percentage and mucilage yield. Results and Discussion:The results of ANOVA showed that nitrogen foliar application on leaf dry weight, calycle fresh and dry weight was effective. Plant fresh weight, dry weight, stem diameter, plant height, mucilage percentageandmucilage yield showedsignificanteffects. Zinc foliar application significantly affected leaf fresh weight,leafdry weight, calycle fresh weight, plant fresh weight,plant dry weight, mucilage percentage andmucilage yield.The interaction effect of nitrogen and zinc on leaf dry weight, plant freshweight and plant dry weight was also significant. The mean comparison of studied characteristics revealed that byincreasing the amount of nitrogen up to N2 level, the stem diameter, plant height, leaf dry weight, calycle dry weight, mucilage percentage and yield increased but there was no significant difference between N2 and N3 levels. Plant fresh weight and plantdry weight

  4. Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage.

    Science.gov (United States)

    Adeyemi, David O; Ukwenya, Victor O; Obuotor, Efere M; Adewole, Stephen O

    2014-07-30

    Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats. Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved in 0.1 M citrate buffer (pH 6.3). The ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76 ± 0.38 μM, 0.42 ± 0.04 U/L, 41.08 ± 3.04 U/ml, 0.82 ± 0.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa-treated rats (6.87 ± 0.51 μM, 0.72 ± 0.06 U/L, 87.92 ± 5.26 U/ml, 1.37 ± 0.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats. The anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.

  5. Volatiles and primary metabolites profiling in two Hibiscus sabdariffa (roselle) cultivars via headspace SPME-GC-MS and chemometrics.

    Science.gov (United States)

    Farag, Mohamed A; Rasheed, Dalia M; Kamal, Islam M

    2015-12-01

    Hibiscus sabdariffa (roselle) is a plant of considerable commercial importance worldwide as functional food due to its organic acids, mucilage, anthocyanins, macro and micro-nutrients content. Although Hibiscus flowers are emerging as very competitive targets for phytochemical studies, very little is known about their volatile composition and or aroma, such knowledge can be suspected to be relevant for understanding its olfactory and taste properties. To provide insight into Hibiscus flower aroma composition and for its future use in food and or pharmaceutical industry, volatile constituents from 2 cultivars grown in Egypt, viz. Aswan and Sudan-1 were profiled using solid-phase microextraction (SPME) coupled to GCMS. A total of 104 volatiles were identified with sugar and fatty acid derived volatiles amounting for the major volatile classes. To reveal for cultivar effect on volatile composition in an untargeted manner, multivariate data analysis was applied. Orthogonal projection to latent structures-discriminant analysis (OPLS-DA) revealed for 1-octen-3-ol versus furfural/acetic acid enrichment in Aswan and Sudan-1 cvs., respectively. Primary metabolites contributing to roselle taste and nutritional value viz. sugars and organic acids were profiled using GC-MS after silylation. The impact of probiotic bacteria on roselle infusion aroma profile was further assessed and revealed for the increase in furfural production with Lactobacillus plantarum inoculation and without affecting its anthocyanin content. This study provides the most complete map for volatiles, sugars and organic acids distribution in two Hibiscus flower cultivars and its fermented product. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Hibiscus sabdariffa L. in the treatment of hypertension and hyperlipidemia: a comprehensive review of animal and human studies

    OpenAIRE

    Hopkins, Allison L.; Lamm, Marnie G.; Funk, Janet; Ritenbaugh, Cheryl

    2013-01-01

    The effectiveness of Hibiscus sabdariffa L. (HS) in the treatment of risk factors associated with cardiovascular disease is assessed in this review by taking a comprehensive approach to interpreting the randomized clinical trial (RCT) results in the context of the available ethnomedical, phytochemical, pharmacological, and safety and toxicity information. HS decoctions and infusions of calyxes, and on occasion leaves, are used in at least 10 countries worldwide in the treatment of hypertensio...

  7. CARACTERIZACIÓN QUÍMICA Y COLORIMÉTRICA DE CULTIVARES DE JAMAICA (Hibiscus sabdariffa L.)

    OpenAIRE

    Camelo Méndez, Gustavo Adolfo

    2013-01-01

    La jamaica (Hibiscus sabdariffa L.) se destaca por su alto contenido en antocianinas y potencial actividad antioxidante. En la medicina tradicional se usa como: bactericida, diurético, cicatrizante y laxante entre otros. Actualmente, en México existen programas de mejoramiento genético tradicional de materiales, que han generado materiales de esta planta con diversos grados de pigmentación y tolerancia a plagas y enfermedades. El objetivo de este trabajo fue caracterizar químic...

  8. Antioxidant and Cytotoxic Effect of Barringtonia racemosa and Hibiscus sabdariffa Fruit Extracts in MCF-7 Human Breast Cancer Cell Line

    OpenAIRE

    Amran, Norliyana; Rani, Anis Najwa Abdul; Mahmud, Roziahanim; Yin, Khoo Boon

    2016-01-01

    Background: The fruits of Barringtonia racemosa and Hibiscus sabdariffa have been used in the treatment of abscess, ulcer, cough, asthma, and diarrhea as traditional remedy. Objective: This study aims to evaluate cytotoxic effect of B. racemosa and H. sabdariffa methanol fruit extracts toward human breast cancer cell lines (MCF-7) and its antioxidant activities. Materials and Methods: Total antioxidant activities of extracts were assayed using 2,2?-diphenyl-1-picrylhydrazyl radical (DPPH) and...

  9. Assessment of nematode community structure as a bioindicator in river monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Wu, H.C.; Chen, P.C. [Department of Plant Pathology, National Chung Hsing University, 250 Kuo Kuan Rd, Taichung 402, Taiwan (China); Tsay, T.T., E-mail: tttsay@nchu.edu.t [Department of Plant Pathology, National Chung Hsing University, 250 Kuo Kuan Rd, Taichung 402, Taiwan (China)

    2010-05-15

    Nematode communities from river water and sediments were assessed for the abundance, feeding types, maturity indices and nematode channel ratio (NCR). The sampling sites studied included different levels of pollution and contamination from agricultural, industrial and sewage sources. The nematode abundance found in the sediment samples was more than that in the water samples. The lowest nematode abundance in sediment samples and the lowest NCR in water samples were both found at the industrial pollution site. Water samples showed positive correlation between the NCR and river pollution index (RPI). Mean maturity indices in sediment samples were inversely correlated with RPI. The pollutant source determined the relationship between NCR and pollution level, while maturity index always showed negative correlation with pollutant level regardless of the pollutant sources. The nematode abundance and its community structure were both reliable bioindicators for monitoring long-term river pollution in both qualitative and quantitative aspects. - Nematode community structure in rivers is related to the contamination source and level.

  10. Assessment of nematode community structure as a bioindicator in river monitoring

    International Nuclear Information System (INIS)

    Wu, H.C.; Chen, P.C.; Tsay, T.T.

    2010-01-01

    Nematode communities from river water and sediments were assessed for the abundance, feeding types, maturity indices and nematode channel ratio (NCR). The sampling sites studied included different levels of pollution and contamination from agricultural, industrial and sewage sources. The nematode abundance found in the sediment samples was more than that in the water samples. The lowest nematode abundance in sediment samples and the lowest NCR in water samples were both found at the industrial pollution site. Water samples showed positive correlation between the NCR and river pollution index (RPI). Mean maturity indices in sediment samples were inversely correlated with RPI. The pollutant source determined the relationship between NCR and pollution level, while maturity index always showed negative correlation with pollutant level regardless of the pollutant sources. The nematode abundance and its community structure were both reliable bioindicators for monitoring long-term river pollution in both qualitative and quantitative aspects. - Nematode community structure in rivers is related to the contamination source and level.

  11. Metal pollution assessment in a Brazilian hydroelectric reservoir: Geophagus brasiliensis as a suitable bioindicator organism

    Directory of Open Access Journals (Sweden)

    Halina Binde Doria

    2017-08-01

    Full Text Available Vossoroca is a reservoir in the Brazilian state of Paraná. Although it is located near big cities and can be used as a human water supply, it has remained unstudied. Concentrations of toxic metals and arsenic in sediments, water, liver, gills, and muscle of Geophagus brasiliensis from the reservoir were analyzed using atomic absorption spectrometry. Histological analyses were performed on the gills and the livers using scanning electron microscopy and light microscopy, respectively. The results showed that Vossoroca sediments were moderately polluted by copper, chromium, nickel and arsenic. Cadmium was above legal limits in the water. Histopathological assessment revealed epithelial alterations in the secondary lamella to be the most common abnormality observed in the gills and necrosis, melanomacrophage centers in the livers. In conclusion, although the reservoir is located in an Environmental Protection Area, it is negatively affected by human activity. Further, Geophagus brasiliensis was a suitable bioindicator for metal pollution studies.

  12. MOSSES AND LICHENS – BIOINDICATORS OF HEAVY METALS POLLUTION OF FOREST ECOSYSTEMS

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    BEGU A.

    2014-03-01

    Full Text Available This study is a comparative investigation of the content of heavy metals (Pb, Cu, Ni, Zn and Cr in mosses and lichens recorded in ten deciduous forests in the Republic of Moldova included in a transnational grid (16x16 km of forest monitoring throughout Europe. The content of heavy metals doesn’t differ significant by depending on the location of studied forest ecosystems. The trends of larger accumulation are observed near the local stationary and mobile sources of pollution. Mosses were confirmed as good indicators of air pollution with heavy metals to forest ecosystems located near sources of pollution and lichens show good bio-indicators particularities for background pollution. The good correlation between the concentrations of moss and lichen were Cr, Cu and Ni, and the low correlation between Pb and Zn, which are considered to be metals which are amenable to long-distance dispersal.

  13. Honey as a bioindicator for environmental pollution with SO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Maja Ponikvar; Joze Snajder; Borka Sedej [Jozef Stefan Institute, Ljubljana (Slovenia)

    2005-07-01

    The authors have proved honey to be a highly sensitive bioindicator for estimation of the environmental pollution with SO{sub 2}. A bee monitoring net consisting of 32 apiaries and covering an area of about 700 km{sup 2} was configured surrounding the Sostanj coal-fired power plant. The results of investigations revealed that the amount of sulphate determined in honey from this area correlated with total yearly emissions of SO{sub 2} observed by an electronic monitoring. When exhaust gases at the power plant were purified, a corresponding decrease in the amounts of sulphate in honey was observed. The proposed bee monitoring net is an example of using biomonitoring for tracing environmental pollution, which could be also applied to estimate the impact of NOx gases or to trace other pollutants.

  14. Diversity of Phytoplankton of the Water Reservoir of Betania-Huila and its Importance as Bioindicators

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    PAULA MARTINEZ SILVA

    2016-08-01

    Full Text Available The Betania reservoir of water is one of the strategic ecosystems of the Huila department, being of vital importance not only for the generation of electricity but for the production of fish which is the main activity for the last thirty years. Nevertheless, activities such as pisiculture, the dumping of wastewater generated by agricultural activities, have diminished the quality of the water and accelerated the eutrophication process. In this research, phytoplankton samples were taken along one year with the objective to stablish through the use of bioindicators, such as microalgae, the actual state of this water body as well as the spatial – temporal variation of species and genres of algae amongst stations and samplings.

  15. Species arboreal as a bioindicator of the environmental pollution: Analysis by SR-TXRF

    Science.gov (United States)

    de Vives, Ana Elisa Sirito; Moreira, Silvana; Brienza, Sandra Maria Boscolo; Medeiros, Jean Gabriel S.; Filho, Mario Tomazello; Zucchi, Orghêda Luiza Araújo Domingues; do Nascimento Filho, Virgilio Franco; Barroso, Regina Cely

    2007-08-01

    This paper aims to study the environmental pollution in the tree development, in order to evaluate its use as bioindicator in urban and countrysides. The sample collection was carried out in Piracicaba city, São Paulo State, that presents high level of environmental contamination in water, soil and air, due to industrial activities, vehicle combustion, sugar-cane leaves burning in the harvesting, etc. The species Caesalpinia peltophoroides ("Sibipiruna") was selected because it is often used in urban arborization. Synchrotron radiation X-ray fluorescence technique (SR-TXRF) was employed to identify and quantify the elements and metals of nutritional and toxicological importance in the wood samples. The analysis was performed in the Brazilian Synchrotron Light Source Laboratory, using a white beam for excitation and an Si(Li) detector for X-ray detection. In several samples were quantified P, K, Ca, Ti, Fe, Sr, Ba and Pb elements.

  16. Earthworms as bio-indicators of chemical pollution in soils with drilling waste

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    Kujawska Justyna

    2017-01-01

    Full Text Available The deposited drilling waste may alter the functioning of soil ecosystems by disrupting the soil fauna. The wastes contain toxic compounds such as metals or hydrocarbons, which tend to accumulate in the living. In the research, earthworms were used u as bio-indicators of soil pollution resulting from the presence of pollution contained in drilling waste. The aim of this study is to evaluation of toxicity of the waste of spent oil and water drilling cuttings to earthworm Eisenia fetida. Oil-based and water-based drilling cuttings were added to soil, used six doses of drilling wastes (5%, 10%, 15%, 25%, 30%, 50%. The results showed that the 45 days LC50 of oil-based drilling cuttings ranged 18%, LC50 of water-based drilling cuttings ranged 33%. In general, mortality increases with increasing dosage waste.

  17. Benthic assemblages, biodiversity and invasiveness in marinas and commercial harbours: an investigation using a bioindicator group.

    Science.gov (United States)

    Megina, Cesar; González-Duarte, Manuel M; López-González, Pablo J

    2016-01-01

    Fouling communities on artificial marine structures are generally different from benthic communities in natural rocky habitats. However, they may also differ among different types of artificial structures. Two artificial structures in direct contact with arriving vessels were compared: floating pontoons within recreational marinas, and sea-walls within commercial harbours. Natural rocky habitats were used as a reference, and the genus Eudendrium (Cnidaria, Hydrozoa) was chosen as a bioindicator. The assemblages were different among the three types of habitat studied, with different species characterising each habitat. The probability of finding an invasive Eudendrium species was significantly higher on pontoons. Diversity was the lowest on pontoons, but it was not significantly different between sea-walls and natural rocks. In general, a barrier to the spread of exotic species exists between harbours and natural rocky habitats. Floating pontoons seem to be a less suitable habitat for native fauna and a key element in marine biological invasions.

  18. Global pollution monitoring of butyltin compounds using skipjack tuna as a bioindicator

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, D.; Inoue, S.; Takahashi, S.; Ikeda, K.; Tanaka, H.; Subramanian, A.N.; Fillmann, G.; Lam, P.K.S.; Zheng, J.; Muchtar, M.; Prudente, M.; Chung, K.; Tanabe, S

    2004-01-01

    Global pollution monitoring of butyltin in offshore water and open sea were conducted using skipjack tuna as a bioindicator. - Butyltin compounds (BTs) including mono- (MBT), di- (DBT), tri-butyltin (TBT) and total tin ({sigma}Sn), were determined in the liver of skipjack tuna (Katsuwonus pelamis) collected from Asian offshore waters (off-Japan, the Japan Sea, off-Taiwan, the East China Sea, the South China Sea, off-Philippines, off-Indonesia, the Bay of Bengal), off-Seychelles, off-Brazil and open seas (the North Pacific). BTs were detected in all the skipjack tuna collected, suggesting widespread contamination of BTs even in offshore waters and open seas on a global scale. Considering specific accumulation, Sex-, body length- differences and migration of skipjack tuna did not seem to affect BT concentrations, indicating rapid reflection of the pollution levels in seawater where and when they were collected. Skipjack tuna is a suitable bioindicator for monitoring the global distribution of BTs in offshore waters and open seas. High concentrations of BTs were observed in skipjack tuna from offshore waters around Japan, a highly developed and industrialized region (up to 400 ng/g wet weight). Moreover skipjack tuna collected from offshore waters around Asian developing countries also revealed the levels comparable to those in Japan (up to 270 ng/g wet weight) which may be due to the recent improvement in economic status in Asian developing countries. High percentages (almost 90%) of BTs in total tin ({sigma}Sn: sum of inorganic tin+organic tin) were found in the liver of skipjack tuna from offshore waters around Asian developing countries. This finding suggests that the anthropogenic BTs represent the major source of Sn accumulation in skipjack tuna from these regions.

  19. Omics technologies and their applications to evaluate metal toxicity in mice M. spretus as a bioindicator.

    Science.gov (United States)

    García-Sevillano, Miguel Ángel; García-Barrera, Tamara; Abril, Nieves; Pueyo, Carmen; López-Barea, Juan; Gómez-Ariza, José Luis

    2014-06-02

    Metals are important components of living organisms since many biological functions critically depend on their interaction with some metal in the cell. However, human activities have increased toxic metal levels in the terrestrial and aquatic ecosystems affecting living organisms. The impact of metals on cellular metabolism and global homeostasis has been traditionally assessed in free-living organisms by using conventional biomarkers; however, to obtain a global vision of metal toxicity mechanisms and the responses that metals elicit in the organisms, new analytical methodologies are needed. We review the use of omics approaches to assess the response of living organisms under metal stress illustrating the possibilities of different methodologies on the basis of our previous results. Most of this research has been based on free-living mice Mus spretus, a conventional bioindicator used to monitor metal pollution in Doñana National Park (DNP) (SW Spain), which is an important European biological reserve for migrating birds affected by agricultural, mining and industrial activities. The benefits of using omic techniques such as heterologous microarrays, proteomics methodologies (2-DE, iTRAQ®), metallomics, ionomics or metabolomics has been remarked; however, the complexity of these areas requires the integration of omics to achieve a comprehensive assessment of their environmental status. This article is part of a Special Issue entitled: Environmental and structural proteomics. This work presents new contributions in the study of environmental metal pollution in terrestrial ecosystems using Mus spretus mice as bioindicator in Doñana National Park (SW Spain) and surroundings. In addition, it has been demonstrated that the integration of omics multi-analytical approaches provides a very suitable approach for the study of the biological response and metal interactions in exposed and free-living mice (Mus musculus and Mus spretus, respectively) under metal pollution

  20. The invasive macrophyte Pistia stratiotes L. as a bioindicator for water pollution in Lake Mariut, Egypt.

    Science.gov (United States)

    Galal, Tarek M; Farahat, Emad A

    2015-11-01

    The present study was conducted to evaluate the potentiality of the aquatic macrophyte Pistia stratiotes to accumulate trace metals, perspective of phytoremediation, and the probability for using it as a bioindicator for the different pollution types. Plants were collected from the different Lake Mariut basins (main basin, south-west, north-west, and fish farm), through five quadrats each, for measuring some growth parameters such as plant density, rosette diameter and height, root length, number of living and dead leaves per individual, and leaf length and width. In addition, nutrients and heavy metals in plant organs as well as water samples were analyzed. The bioaccumulation and translocation factors of trace metals were calculated. Water physicochemical data of Lake Mariut showed significant variations of all variables, except temperature and pH as well as Cd metal, among the lake basins. Fish farm was characterized by the highest plant density, individual size, biomass, and the number of living leaves, while the north-west basin had the lowest, except the number of dead leaves. In contrast to trace metals, P. stratiotes accumulated concentrations of macronutrients in the leaves higher than in roots. The bioaccumulation factors of the investigated metals, except Cu, were greater than one, while the translocation factors (TFs) of all trace metals were less than unity, and this may render P. stratiotes suitable for rhizofiltration. In addition, the significant positive correlation of Ni and Cd in water with those in plant roots and leaves as well as the growth response of this plant to the different pollutants may suggest its potential use as bioindicator for these pollutants in water.

  1. Assessment of clam ruditapes philippinarum as Heavy metal bioindicators using NMR-based metabolomics

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xiaoli; Zhang, Linbao; You, Liping [Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai (China); The Graduate School of Chinese Academy of Sciences, Beijing (China); Yu, Junbao; Cong, Ming; Wang, Qing; Li, Fei; Li, Lianzhen; Zhao, Jianmin; Li, Chenghua; Wu, Huifeng [Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai (China)

    2011-08-15

    There are mainly distributed three pedigrees (White, Liangdao Red, and Zebra) of Manila clam Ruditapes philippinarum in Yantai population along the Bohai marine and coast. However, the biological differences to environmental stressors have been ignored in toxicology studies, which could lead to the distortion of biological interpretations of toxicological effects induced by environmental contaminants. In this study, we applied a system biology approach, metabolomics to compare the metabolic profiles in digestive gland from three pedigrees of clam and characterize and compare the metabolic responses induced by mercury in clam digestive gland tissues to determine a sensitive pedigree of clam as a preferable bioindicator for metal pollution monitoring and toxicology research. The most abundant metabolites, respectively, included branched-chain amino acids, alanine, and arginine in White samples, glutamate, dimethylglycine, and glycine in Zebra clams and acetylcholine, betaine, glucose, and glycogen in Liangdao Red clams. After 48 h exposure of 20 {mu}g L{sup -1} Hg{sup 2+}, the metabolic profiles from the three pedigrees of clams showed differentially significant changes in alanine, glutamate, succinate, taurine, hypotaurine, glycine, arginine, glucose, etc. Our findings indicate the toxicological effects of mercury exposure in Manila clams including the neurotoxicity, disturbances in energetic metabolisms and osmoregulation in the digestive glands and suggest that Liangdao Red pedigree of clam could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive classes of metabolic changes from digestive glands compared with other two (White and Zebra) pedigrees of clams. (Copyright copyright 2011 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  2. Systematic Radioactivity Monitoring of Adriatic Coastal Waters Using Mussels (Mytilus Galloprovincialis) as a Bioindicator

    International Nuclear Information System (INIS)

    Barisic, D.; Rogic, M.; Rozmaric Macefat, M.; Benedik, Lj.; Strok, M.

    2013-01-01

    Mediterranean Mussel Watch (MMW) is a project for radioactivity monitoring of Mediterranean coastal waters using mussels (Mytilus galloprovincialis) as a bioindicator. Mussels are sessile marine organisms able to filter up to 80 L of sea water daily. Possibility of absorption and accumulation of dissolved/particulate matter from sea water makes them a good bioindicator for environmental pollution studies. Laboratory for Radioecelogy systematically monitores Adriatic coastal waters as a part of MMW project, which includes determination of 7Be, 40K, 232Th, 226Ra,238U and 137Cs, as well as highly radiotoxic naturally occuring radionuclides 210Po and 210Pb. The mussels were collected in spring and autumn periods of 2010 and 2011 at 13 stations including areas under significant fresh water discharges (Rasa, Zrnovnica, Neretva and Ombla Rivers) or areas under potential antropogenic influence (Kastela Bay). After sample preparation 7Be, 40K, 232Th, 226Ra, 238U and 137Cs were determined gamma-spectrometrically, while 210Po and 210Pb were separated on Sr resin. 210Po was determined on an alpha spectrometer after self-deposition on Ag disc, while 210Pb was determined via 210Bi on a gas proportional counter after PbSO 4 precipitation. 7Be, 210Po and 210Pb activity concentrations were higher in spring than in autumn periods for all locations, with the highest activities in the areas under heavy fresh water discharges. Activity concentrations of 40K were practically the same at all locations with no seasonal changes, while 137Cs activities varied significantly. Activities of 232Th, 238U and 226Ra were mostly below the detection limit of gamma-spectrometric measurements.(author)

  3. Benthic macroinvertebrates as bioindicators of water quality in Billings Reservoir fishing sites (SP, Brazil

    Directory of Open Access Journals (Sweden)

    José Ricardo Baroldi Ciqueto Gargiulo

    Full Text Available Abastract: Aim The Billings Reservoir is the largest reservoir in the metropolitan region of São Paulo and has multiple uses, including artisanal fishing. Its surroundings present intense occupation, resulting in various environmental impacts. Although the water is degraded, affecting the composition and quality of the fish, this reservoir supports artisanal fishermen who survive through this activity. This study aimed to analyze the water quality in the main sites of artisanal fishing activity, with an emphasis on the benthic community as a bioindicator. Methods Three sampling sites were selected, in which water and zoobenthos samples were collected monthly from April 2012 to March 2013. Water analyses consisted of the determination of pH, temperature, conductivity, turbidity, total dissolved solids, dissolved oxygen, total nitrogen, total phosphorus, and trophic state as well as a principal components analysis. The zoobenthos analysis consisted of determining the relative abundance and total density of taxa, the Shannon-Weaver diversity index, taxa richness, uniformity, Simpson’s dominance index, and the benthic community index and conducting a canonical correspondence analysis. The non-parametric Kruskal-Wallis test followed by the Student-Newman-Keuls test were used to investigate the existence of significant differences in the abiotic and biotic variables among the sites. Results The studied sites showed a high degree of eutrophication, with nitrogen and phosphorus totals levels above the limits defined by current Brazilian legislation. Taquacetuba (P1 showed the best environmental conditions for the development of fishing activity, with the presence of sensitive organisms (Polymitarcyidae and higher levels of dissolved oxygen. Alvarenga (P2 had the worst water quality, with a predominance of tolerant organisms (Oligochaeta and lower levels of dissolved oxygen. Conclusion In conclusion, the benthic community in association with abiotic

  4. Sensitivity of aquatic organisms to ethanol and its potential use as bioindicators

    Directory of Open Access Journals (Sweden)

    Adilson Ferreira Silva

    2016-12-01

    Full Text Available The aim of this research was to evaluate the feasibility for the use of the organisms Lemna minor, Azolla caroliniana, Hyphessobrycon eques, Pomacea canaliculata and Daphnia magna as exposure bioindicators for ethanol (lethal and effective concentration 50% - LC50(I/EC50(I. Thus, the following concentrations were used 5.0; 10.0; 20.0; 30.0; 40.0 and 50.0 mg L-1 ethanol on L. minor; 25.0; 50.0; 75.0; 100.0; 150.0 and 200.0 mg L-1 on A. caroliniana; 0.3; 0.5; 1.0; 2.0 and 3.0 mg L-1 on H. eques; 0.05; 0.10; 0.20; 0.40 and 0.80 mg L-1 on P. canaliculata; and 40.0; 60.0; 80.0; 100.0; 120.0 and 140.0 mg L-1 on D. magna. An untreated control was also kept for all organisms, with three repetitions. The increase in the ethanol concentration elevated the electrical conductivity and decreased the water dissolved oxygen and pH. The ethanol LC50 for L. minor and A. caroliniana were 12.78 and 73.11 mg L-1, respectively, and was classified as slightly toxic; 1.22 mg L-1 for H. eques (moderately toxic; 0.39 mg L-1 for P. canaliculata (highly toxic and 98.85 mg L-1 for D. magna (slightly toxic. Thus, H. eques and P. canaliculata have showed good potential for the use as ethanol exposure bioindicators on water bodies.

  5. A Lymnaea stagnalis Embryo Test for Toxicity Bioindication of Acidification and Ammonia Pollution in Water

    Directory of Open Access Journals (Sweden)

    Robert Mazur

    2016-07-01

    Full Text Available The paper presents a study leading to a new acute toxicity test on embryonic and juvenile organisms of the great pond snail (Lymnaea stagnalis Linnaeus. Sulfuric acid, nitric acid, and ammonium hydroxide were used as waterborne toxicants in laboratory experiments. The exposure time was 24 h. Tests were conducted in 5–10 replications for each toxicant. The toxicity of the substances was classified according to different scales and the test’s sensitivity was compared to that of the commonly used bioindicator Daphnia magna Straus. The assessment of toxicity impact was supported by microscopic observations. The probit method was used as a parametric statistical procedure to estimate LC50 and the associated 95% confidence interval. Our study showed that the early developmental stages of Lymnaea stagnalis are very sensitive bioindicators, making it possible to detect even very low levels of the above-mentioned water toxicants. The highest toxicity is shown by ammonium hydroxide with LC50/24h values, respectively, 24.27 for embryos and 24.72 for juvenile forms, and the lowest is shown by nitric acid ions with LC50/24h values, respectively, 105.19 for embryos and 170.47 for juvenile forms. It is highly cost-effective due to simple and efficient breeding and the small size of the organisms in the bioassay population. Compared with Daphnia magna, relatively low concentrations of toxicants caused a lethal effect on embryonic and juvenile organisms of the great pond snail. Owing to their common occurrence and sensitivity, early developmental forms of Lymnaea stagnalis can be a valuable new tool in biomonitoring of the freshwater environment.

  6. Heavy metals in human teeth dentine: A bio-indicator of metals exposure and environmental pollution.

    Science.gov (United States)

    Asaduzzaman, Khandoker; Khandaker, Mayeen Uddin; Binti Baharudin, Nurul Atiqah; Amin, Yusoff Bin Mohd; Farook, Mohideen Salihu; Bradley, D A; Mahmoud, Okba

    2017-06-01

    With rapid urbanization and large-scale industrial activities, modern human populations are being increasingly subjected to chronic environmental heavy metal exposures. Elemental uptake in tooth dentine is a bioindicator, the uptake occurring during the formation and mineralization processes, stored to large extent over periods of many years. The uptake includes essential elements, most typically geogenic dietary sources, as well as non-essential elements arising through environmental insults. In this study, with the help of the Dental Faculty of the University of Malaya, a total of 50 separate human teeth were collected from dental patients of various ethnicity, age, gender, occupation, dietary habit, residency, etc. Analysis was conducted using inductively coupled plasma-mass spectrometry (ICP-MS), most samples indicating the presence of the following trace elements, placed in order of concentration, from least to greatest: As, Mn, Ba, Cu, Cr, Pb, Zn, Hg, Sb, Al, Sr, Sn. The concentrations have been observed to increase with age. Among the ethnic groups, the teeth of ethnic Chinese showed marginally greater metal concentrations than those of the Indians and Malays, the teeth dentine of females generally showing greater concentrations than that of males. Greater concentrations of Hg, Cu and Sn were found in molars while Pb, Sr, Sb and Zn were present in greater concentrations in incisors. With the elevated concentration levels of heavy metals in tooth dentine reflecting pollution from industrial emissions and urbanization, it is evident that human tooth dentine can provide chronological information on exposure, representing a reliable bio-indicator of environmental pollution. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Narrowband Bio-Indicator Monitoring of Temperate Forest Carbon Fluxes in Northeastern China

    Directory of Open Access Journals (Sweden)

    Quanzhou Yu

    2014-09-01

    Full Text Available Developments in hyperspectral remote sensing techniques during the last decade have enabled the use of narrowband indices to evaluate the role of forest ecosystem variables in estimating carbon (C fluxes. In this study, narrowband bio-indicators derived from EO-1 Hyperion data were investigated to determine whether they could capture the temporal variation and estimate the spatial variability of forest C fluxes derived from eddy covariance tower data. Nineteen indices were divided into four categories of optical indices: broadband, chlorophyll, red edge, and light use efficiency. Correlation tests were performed between the selected vegetation indices, gross primary production (GPP, and ecosystem respiration (Re. Among the 19 indices, five narrowband indices (Chlorophyll Index RedEdge 710, scaled photochemical reflectance index (SPRI*enhanced vegetation index (EVI, SPRI*normalized difference vegetation index (NDVI, MCARI/OSAVI[705, 750] and the Vogelmann Index, and one broad band index (EVI had R-squared values with a good fit for GPP and Re. The SPRI*NDVI has the highest significant coefficients of determination with GPP and Re (R2 = 0.86 and 0.89, p < 0.0001, respectively. SPRI*NDVI was used in atmospheric inverse modeling at regional scales for the estimation of C fluxes. We compared the GPP spatial patterns inversed from our model with corresponding results from the Vegetation Photosynthesis Model (VPM, the Boreal Ecosystems Productivity Simulator model, and MODIS MOD17A2 products. The inversed GPP spatial patterns from our model of SPRI*NDVI had good agreement with the output from the VPM model. The normalized difference nitrogen index was well correlated with measured C net ecosystem exchange. Our findings indicated that narrowband bio-indicators based on EO-1 Hyperion images could be used to predict regional C flux variations for Northeastern China’s temperate broad-leaved Korean pine forest ecosystems.

  8. Hibiscus rosa sinensis Linn. Petals Modulates Glycogen Metabolism and Glucose Homeostasis Signalling Pathway in Streptozotocin-Induced Experimental Diabetes.

    Science.gov (United States)

    Pillai, Sneha S; Mini, S

    2016-03-01

    The prevalence of diabetes mellitus is becoming more and more serious and reaches epidemic proportions worldwide. Scientific research is constantly looking for new agents that could be used as dietary functional ingredients in the fight against diabetes. The objective of the present study was to evaluate the effect of ethyl acetate fraction of Hibiscus rosa sinensis Linn. petals on experimental diabetes at a dose of 25 mg/kg body weight and it was compared with standard anti-diabetic drug metformin. The elevated levels of serum glucose (398.56 ± 35.78) and glycated haemoglobin (12.89 ± 1.89) in diabetic rats were significantly decreased (156.89 ± 14.45 and 6.12 ± 0.49, respectively) by Hibiscus rosa sinensis petals (EHRS) administration. Hepatotoxicity marker enzyme levels in serum were normalized. The fraction supplementation restored the glycogen content by regulating the activities of glycogen metabolizing enzymes. It significantly modulated the expressions of marker genes involved in glucose homeostasis signalling pathway. Histopathological analysis of liver and pancreas supported our findings. The overall effect was comparable with metformin. Hence, our study reveals the role of hibiscus petals for alleviation of diabetes complications, thus it can be propagated as a nutraceutical agent.

  9. The acute impact of polyphenols from Hibiscus sabdariffa in metabolic homeostasis: an approach combining metabolomics and gene-expression analyses.

    Science.gov (United States)

    Beltrán-Debón, Raúl; Rodríguez-Gallego, Esther; Fernández-Arroyo, Salvador; Senan-Campos, Oriol; Massucci, Francesco A; Hernández-Aguilera, Anna; Sales-Pardo, Marta; Guimerà, Roger; Camps, Jordi; Menendez, Javier A; Joven, Jorge

    2015-09-01

    We explored the acute multifunctional effects of polyphenols from Hibiscus sabdariffa in humans to assess possible consequences on the host's health. The expected dynamic response was studied using a combination of transcriptomics and metabolomics to integrate specific functional pathways through network-based methods and to generate hypotheses established by acute metabolic effects and/or modifications in the expression of relevant genes. Data were obtained from healthy male volunteers after 3 hours of ingestion of an aqueous Hibiscus sabdariffa extract. The data were compared with data obtained prior to the ingestion, and the overall findings suggest that these particular polyphenols had a simultaneous role in mitochondrial function, energy homeostasis and protection of the cardiovascular system. These findings suggest beneficial actions in inflammation, endothelial dysfunction, and oxidation, which are interrelated mechanisms. Among other effects, the activation of the heme oxygenase-biliverdin reductase axis, the systemic inhibition of the renin-angiotensin system, the inhibition of the angiotensin-converting enzyme, and several actions mirroring those of the peroxisome proliferator-activated receptor agonists further support this notion. We also found concordant findings in the serum of the participants, which include a decrease in cortisol levels and a significant increase in the active vasodilator metabolite of bradykinin (des-Arg(9)-bradykinin). Therefore, our data support the view that polyphenols from Hibiscus sabdariffa play a regulatory role in metabolic health and in the maintenance of blood pressure, thus implying a multi-faceted impact in metabolic and cardiovascular diseases.

  10. High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.

    Science.gov (United States)

    Baatartsogt, Tugsbaatar; Bui, Vuong N; Trinh, Dai Q; Yamaguchi, Emi; Gronsang, Dulyatad; Thampaisarn, Rapeewan; Ogawa, Haruko; Imai, Kunitoshi

    2016-10-01

    Viral neuraminidase inhibitors are widely used as synthetic anti-influenza drugs for the prevention and treatment of influenza. However, drug-resistant influenza A virus variants, including H5N1 highly pathogenic avian influenza viruses (HPAIVs), have been reported. Therefore, the discovery of novel and effective antiviral agents is warranted. We screened the antiviral effects of 11 herbal tea extracts (hibiscus, black tea, tencha, rosehip tea, burdock tea, green tea, jasmine tea, ginger tea, lavender tea, rose tea and oak tea) against the H5N1 HPAIV in vitro. Among the tested extracts, only the hibiscus extract and its fractionated extract (frHibis) highly and rapidly reduced the titers of all H5 HPAIVs and low pathogenic AIVs (LPAIVs) used in the pre-treatment tests of Madin-Darby canine kidney (MDCK) cells that were inoculated with a mixture of the virus and the extract. Immunogold electron microscopy showed that anti-H5 monoclonal antibodies could not bind to the deformed H5 virus particles pretreated with frHibis. In post-treatment tests of MDCK cells cultured in the presence of frHibis after infection with H5N1 HPAIV, the frHibis inhibited viral replication and the expression of viral antigens and genes. Among the plants tested, hibiscus showed the most prominent antiviral effects against both H5 HPAIV and LPAIV.

  11. Acclimation of croton and hibiscus seedlings in response to the application of indobultiric acid and humic acid for rooting

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2015-06-01

    Full Text Available The vegetative propagation of ornamental plants can be accelerated by applying plant growth regulators. Amongst them, the use of auxins, plant hormones with physiological effects on cell elongation and rooting have stood out. Alternatively, the application of humic acids, bioactive fraction of soil organic matter, also results in increases in rooting cuttings of ornamental plants. The objective of this work was to study the growth characteristics and the nutritional contents of croton and hibiscus plants during acclimation of seedlings in response to different concentrations of indolebutyric acid (IBA and humic acid (HA applied to cuttings for rooting. The experiment was conducted in greenhouse, and the apical stem cuttings were treated with solutions with concentrations of 0, 250, 500, 1000 and 2000 mg L-1of IBA and 0, 10, 20, 30 and 40 mg L-1 of C from HA. At 45 days of rooting in carbonized rice husk, they were individually transferred to plastic bags of 2.0 dm3 containing a mixture of soil: sand: manure (2: 1: 1 as substrate. At 90 days of acclimation, the plants were collected for measurement of growth and nutritional variables. The results showed that the application of the IBA stimulates the absorption of nutrients and growth of croton cuttings and transplanted hibiscus, contributing to formation of vigorous seedlings. A similar response occurred with the application of HA in hibiscus cuttings

  12. Effect of Hibiscus sabdariffa Calices on Dyslipidemia in Obese Adolescents: A Triple-masked Randomized Controlled Trial

    Science.gov (United States)

    Sabzghabaee, Ali Mohammad; Ataei, Ehsan; Kelishadi, Roya; Ghannadi, Alireza; Soltani, Rasool; Badri, Shirinsadat; Shirani, Shahin

    2013-01-01

    Conflict of interest: none declared. Objective We aimed to evaluate the effects of Hibiscus sabdariffa (HS) calices on controlling dyslipidemia in obese adolescents. Methodology In this triple blind randomized placebo-controlled clinical trial which was registered in the Iranian registry for clinical trials (IRCT201109122306N2), 90 obese adolescents aged 12-18 years with documented dyslipidemia were randomly assigned in two groups of cases who received 2 grams of fine powdered calices of Hibiscus sabdariffa per day for one month and controls who received placebo powder with the same dietary and physical activity recommendations and duration of exposure. Full lipid profile and fasting blood sugar measured before and after the trial. Data were analyzed using multivariate general linear model. Findings Overall, 72 participants (mean age of 14.21±1.6, 35 boys) completed the trial. The two arms of the study (cases and controls) were not statistically different in terms of age, gender, weight, body mass index (BMI) and lipid profile before the trial. Serum total cholesterol, low density lipoprotein cholesterol and serum triglyceride showed a significant decrease in cases group but high density lipoprotein cholesterol level was not changed significantly. Conclusion It is concluded that Hibiscus sabdariffa calyces powder may have significant positive effects on lipid profile of adolescents which maybe attributed to its polyphenolic and antioxidant content. Further studies are needed on dose-response and formulation optimization. PMID:24082826

  13. Evaluation of the Trophic Level of Kune and Vain Lagoons in Albania, Using Phytoplankton as a Bioindicator

    Directory of Open Access Journals (Sweden)

    Anni Koci Kallfa

    2014-03-01

    Full Text Available Concentration of chlorophyll is an adequate parameter for assessing the trophic state of lagoon ecosystems. Objectives of this study are: selection of a system of bioindicators to enable a good qualitative evaluation of the trophic state of the lagoons and their dynamics; evaluation of seasonal water quality variability and comparison between lagoons. The trophic state of the lagoons is analysed every month over the year. Water samples are retrieved at four different sites (exact coordinates each month, sites that are representative of different water circulation systems at each lagoon. The trophic level in the respective lagoons is thus assessed through selection of an adequate system of bioindicators, in order to observe the oscillations of the amount of chlorophyll and therefore to determine the level of eutrophication. Based on the above parameters, the comparison of the trophic state in these two lagoons has shown that they have different trophic states.

  14. Hibiscus sabdariffa

    African Journals Online (AJOL)

    China, in Sudan, the oil is consumed and also used in the production of poultry feeds (Al-. Wandawi et al., 1984). In many part of the world including Nigeria, Roselle seeds are being wasted as there is no such value addition as the established or large scale extraction of its oil. The major importance of vegetable oils lies in ...

  15. Hibiscus cannabinus

    African Journals Online (AJOL)

    morufat

    2013-05-08

    May 8, 2013 ... embryogenic callus clusters were recorded. Flowering was delayed by at least 27 days in V400 relative to other genotypes. Growth rate reduced by 30% after flowering in all genotypes except V400 where it increased by 60%. Highest yield of 76 g / plant was recorded in V400. Eighty-four percent degree of.

  16. The Tropical Brown Alga Lobophora variegata (Lamouroux) Womersley: A Prospective Bioindicator for Ag Contamination in Tropical Coastal Waters

    OpenAIRE

    Metian, Marc; Warnau, Michel

    2008-01-01

    International audience; Uptake and depuration kinetics of dissolved silver were determined in the brown alga Lobophora variegata, using radiotracer techniques. Results indicate that this widely distributed alga could be a useful bioindicator species for surveying silver contamination in tropical environments. Indeed, results showed that the alga readily concentrates silver (algal concentration of silver was 7,000 times higher than in water after a 28-day exposure) and retains it efficiently w...

  17. Radioactively contaminated areas: Bioindicator species and biomarkers of effect in an early warning scheme for a preliminary risk assessment

    Energy Technology Data Exchange (ETDEWEB)

    Lourenço, Joana, E-mail: joanalourenco@ua.pt [Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro (Portugal); Mendo, Sónia [Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro (Portugal); Pereira, Ruth [Department of Biology, Faculty of Sciences of the University of Porto & CIIMAR – Interdisciplinary Centre of Marine and Environmental Research & GreenUP/CITAB-UP, Porto (Portugal)

    2016-11-05

    Highlights: • Knowing the most used biomarkers and bioindicators used in radioactive areas. • Understanding of the response similarities between human and non-human biota. • Identifying the knowledge gaps. • Proposing an early warning scheme, to perform a screening evaluation of radioactive areas. • Permitting routine assessments without disturbing and alarming local populations. - Abstract: Concerns about the impacts on public health and on the natural environment have been raised regarding the full range of operational activities related to uranium mining and the rest of the nuclear fuel cycle (including nuclear accidents), nuclear tests and depleted uranium from military ammunitions. However, the environmental impacts of such activities, as well as their ecotoxicological/toxicological profile, are still poorly studied. Herein, it is discussed if organisms can be used as bioindicators of human health effects, posed by lifetime exposure to radioactively contaminated areas. To do so, information was gathered from several studies performed on vertebrates, invertebrate species and humans, living in these contaminated areas. The retrieved information was compared, to determine which are the most used bioindicators and biomarkers and also the similarities between human and non-human biota responses. The data evaluated are used to support the proposal for an early warning scheme, based on bioindicator species and on the most sensitive and commonly shared biomarkers, to perform a screening evaluation of radioactively contaminated sites. This scheme could be used to support decision-making for a deeper evaluation of risks to human health, making it possible to screen a large number of areas, without disturbing and alarming local populations.

  18. Calotropis procera seedlings could be used as a rapid cost effective bioindicator for measuring aluminum environmental pollution

    Science.gov (United States)

    Mosa, Kareem A.; El-Keblawy, Ali; Najar, Atyat

    2017-04-01

    Calotropis procera seedlings could be used as a rapid cost effective bioindicator for measuring aluminum environmental pollution Kareem A. Mosa, Ali El-Keblawy, Atyat Najar Department of Applied Biology, College of Sciences, University of Sharjah, UAE Rapid industrialization and urbanization processes has led to the incorporation of different heavy metals in natural resources like soil, water and air thus affecting their quality. Aluminum (Al) is a dominant heavy metal pollutant that causes serious toxic effects to living systems including plants. Therefore, it is critical to regularly monitor the changes in Al levels in natural resources. Living organisms could be used as bioindicators for monitoring and measuring the levels of heavy metals in environmental samples. The aim of this study was to develop a cost effective bioindicator for monitoring aluminum (Al) and assess the damage caused by Al bioaccumulation using the root system of Calotropis Procera seedlings. A hydroponic system was developed for growing C. Procera in four different concentrations of Al (20, 40, 60 and 80 ppm). Root length and shoot fresh and dry weights were assessed after 5, 10, 15 and 20 days of Al treatment. The results showed remarkable sensitivity of C. Procera seedlings for the different concentrations of Al. There was gradual but significant decrease in C. Procera root length with the increase in the Al concentrations. X-ray fluorescence microscopy (XRF) analysis indicated a significant increase in Al concentration in C. Procera roots with the increase of both Al concentration in the hydroponic solution and the growing period. Moreover, electrical conductivity analysis showed that Al induced damage to C. Procera root plasma membrane as indicated by the increase in electrolyte leakages. Randomly amplified polymorphic DNA (RAPD) PCR analysis confirmed the genotoxin effect of Al which induced C. Procera genomic DNA modification. Altogether, the result demonstrated that C. Procera could

  19. A model for selecting bioindicators to monitor radionuclide concentrations using Amchitka Island in the Aleutians as a case study

    International Nuclear Information System (INIS)

    Burger, Joanna

    2007-01-01

    World War II and the Cold War have left the Unites States, and other Nations, with massive cleanup and remediation tasks for radioactive and other legacy hazardous wastes. While some sites can be cleaned up to acceptable residential risk levels, others will continue to hold hazardous wastes, which must be contained and monitored to protect human health and the environment. While media (soil, sediment, groundwater) monitoring is the usual norm at many radiological waste sites, for some situations (both biological and societal), biomonitoring may provide the necessary information to assure greater peace of mind for local and regional residents, and to protect ecologically valuable buffer lands or waters. In most cases, indicators are selected using scientific expertise and a literature review, but not all selected indicators will seem relevant to stakeholders. In this paper, I provide a model for the inclusion of stakeholders in the development of bioindicators for assessing radionuclide levels of biota in the marine environment around Amchitka Island, in the Aleutian Chain of Alaska. Amchitka was the site of three underground nuclear tests from 1965 to 1971. The process was stakeholder-initiated, stakeholder-driven, and included stakeholders during each phase. Phases included conceptualization, initial selection of biota and radionuclides, refinement of biota and radionuclide target lists, collection of biota, selection of biota and radionuclides for analysis, and selection of biota, tissues, and radionuclides for bioindicators. The process produced site-specific information on biota availability and on radionuclide levels that led to selection of site-appropriate bioindicators. I suggest that the lengthy, iterative, stakeholder-driven process described in this paper results in selection of bioindicators that are accepted by biologists, public health personnel, public-policy makers, resource agencies, regulatory agencies, subsistence hunters/fishers, and a wide

  20. A model for selecting bioindicators to monitor radionuclide concentrations using Amchitka Island in the Aleutians as a case study.

    Science.gov (United States)

    Burger, Joanna

    2007-11-01

    World War II and the Cold War have left the Unites States, and other Nations, with massive cleanup and remediation tasks for radioactive and other legacy hazardous wastes. While some sites can be cleaned up to acceptable residential risk levels, others will continue to hold hazardous wastes, which must be contained and monitored to protect human health and the environment. While media (soil, sediment, groundwater) monitoring is the usual norm at many radiological waste sites, for some situations (both biological and societal), biomonitoring may provide the necessary information to assure greater peace of mind for local and regional residents, and to protect ecologically valuable buffer lands or waters. In most cases, indicators are selected using scientific expertise and a literature review, but not all selected indicators will seem relevant to stakeholders. In this paper, I provide a model for the inclusion of stakeholders in the development of bioindicators for assessing radionuclide levels of biota in the marine environment around Amchitka Island, in the Aleutian Chain of Alaska. Amchitka was the site of three underground nuclear tests from 1965 to 1971. The process was stakeholder-initiated, stakeholder-driven, and included stakeholders during each phase. Phases included conceptualization, initial selection of biota and radionuclides, refinement of biota and radionuclide target lists, collection of biota, selection of biota and radionuclides for analysis, and selection of biota, tissues, and radionuclides for bioindicators. The process produced site-specific information on biota availability and on radionuclide levels that led to selection of site-appropriate bioindicators. I suggest that the lengthy, iterative, stakeholder-driven process described in this paper results in selection of bioindicators that are accepted by biologists, public health personnel, public-policy makers, resource agencies, regulatory agencies, subsistence hunters/fishers, and a wide

  1. Bio-raffinage de plantes aromatiques et médicinales appliqué à l'Hibiscus sabdariffa L. et à l'Artemisia annua

    OpenAIRE

    Endrias, Abraham

    2006-01-01

    L'objectif de l'étude était d'évaluer les potentialités aromatiques et médicinales de deux plantes (Hibiscus sabdariffa et Artemisia annua). Les performances et rendement de plusieurs méthodes d'extraction et de solvants sur des calices et des graines d'hibiscus, issus de trois pays (Vietnam, Sénégal et Mexique) ont été comparés. Les profils en composés volatifs diffèrent selon les extraits. La teneur en acide gras, stérols et tocophérols des huiles des graines d'hibiscus est comparable à cel...

  2. Radioactively contaminated areas: Bioindicator species and biomarkers of effect in an early warning scheme for a preliminary risk assessment.

    Science.gov (United States)

    Lourenço, Joana; Mendo, Sónia; Pereira, Ruth

    2016-11-05

    Concerns about the impacts on public health and on the natural environment have been raised regarding the full range of operational activities related to uranium mining and the rest of the nuclear fuel cycle (including nuclear accidents), nuclear tests and depleted uranium from military ammunitions. However, the environmental impacts of such activities, as well as their ecotoxicological/toxicological profile, are still poorly studied. Herein, it is discussed if organisms can be used as bioindicators of human health effects, posed by lifetime exposure to radioactively contaminated areas. To do so, information was gathered from several studies performed on vertebrates, invertebrate species and humans, living in these contaminated areas. The retrieved information was compared, to determine which are the most used bioindicators and biomarkers and also the similarities between human and non-human biota responses. The data evaluated are used to support the proposal for an early warning scheme, based on bioindicator species and on the most sensitive and commonly shared biomarkers, to perform a screening evaluation of radioactively contaminated sites. This scheme could be used to support decision-making for a deeper evaluation of risks to human health, making it possible to screen a large number of areas, without disturbing and alarming local populations. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Ecotoxicological evaluation of water of the hydrographic Basin of the Una River using the bioindicator Ceriodaphnia dubia

    Directory of Open Access Journals (Sweden)

    Tatiane Alves

    2013-12-01

    Full Text Available The majority of the Una River Basin is located in Taubaté County and contributes significantly to its water supply. The main goal of this research was to evaluate the water quality of the Una River using the microcrustacean C. dubia as bioindicator for tests of chronic and acute toxicity. Bimonthly water samples were obtained from each of six localities throughout the Una Basin, from March to October, 2011. Physical-chemical water parameters such as pH, electrical conductivity, hardness, dissolved oxygen and precipitation were measured and correlated to the C. dubia reproductive rates. No significant relationships were found between the water’s electrical conductivity and precipitation with respect to bioindicator reproductive rates. However, at the Sete Voltas, Antas and Rocinha Sub-Basins, significant interactions were detected between some water parameters and reproductive rates, suggesting that water may constrain the reproduction of C. dubia. Acute toxicity was not detected in any of the six sites, while chronic toxicity was recorded at Rocinha, Sete Voltas, Antas, Médio and Baixo Una Sub-Basins. In general, the water quality of the Una Basin, as indicated by the absence of acute toxicity, still remains in an acceptable conservation condition. Caution is needed, however, since slight pollution sources are causing chronic toxicity in some localities. In addition, as the microcrustacean C. dubia, appeared to be a reliable bioindicator in this investigation, we suggest that it be used for continuous water quality monitoring programs.

  4. Estudio fitoquímico de la especie Hibiscus elatus S.W.

    Directory of Open Access Journals (Sweden)

    Ingrid Márquez Hernández

    1999-08-01

    Full Text Available Se realizó un estudio fitoquímico preliminar de la especie Hibiscus elatus S.W. Se practicó un tamizaje fitoquímico sobre material seco y fresco, en el que se obtuvieron resultados positivos para mucílagos, sustancias reductoras, antocianinas, aminoácidos, taninos y flavonoides. Se determinó que las antocianinas se afectan durante el proceso de secado. Para la realización del estudio químico se maceró el material vegetal con 4 menstruos (fracciones A, B, C y D. A partir de las fracciones A y B se aisló el producto HE1, el cual se analizó por espectroscopia ultravioleta, infrarroja, de resonancia magnética nuclear protónica, de carbono 13, mediante uso de técnicas especiales y por espectrometría de masas, lo que permitió identificarlo como el flavonoide gossypitrina. La fracción C se sometió a un fraccionamiento según el método de absorción-desorción sobre silicagel, lo que permitió la detección de rutina y quercetina. Los flavonoides identificados se reportan por primera vez para la especie.A preliminary phytochemical study of Hibiscus elatus species was undertaken. A phytochemical sieving of fresch dry material made it possible to obtain positive results for mucilages, reducing substances, anthocyamins, aminoacids, tanines and flavonoids. Anthocyamines were determined to be affected during dryng process. For carry ing uot the chemical study, the plant was mecrated into 4 solvents (A, B, C and D fractions. Taking A and B fractions as a basis, product HE1 was isolated and analyzed by ultra - violet, infrared, nuclear magnetic resonance, proton and carbon 13 spectroscopies as well as special techniques and mass spectrometry. This resulted in the identification of this product as gossypitrin flavonoid. Fraction C was fractioned by silicagel absorption - desorption methods which allowed us to detec rutin and quercetin. The identified flavonoids are reported for the first time in this species.

  5. PENGARUH PEMBERIAN SUBKRONIK EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus Sabdariffa L. TERHADAP KADAR SGPT SGOT DAN ALP

    Directory of Open Access Journals (Sweden)

    Nurkhasanah Nurkhasanah

    2016-11-01

    Full Text Available Roselle (Hibiscus sabdariffa L. has been known as traditional medicinal plants. It needed to evaluate the safety of roselle extract on long-term oral administration. The research aimed to determine the savety of ethanolic extract of roselle calyx. Subchronic toxicity study of ethanolic extract of roselle calyx had been carried out on 60 Sprague Dawleys (SD rats for 28 days. The roselle extract was administered orally every day, with doses of 50,100 and 200 mg/kgBW. There are 2 satellite groups, kept for another 14 days after the treatment in order to detect a delayed occurrence of toxic effect. Satellite group was given extract 200 mg/kgBW for 28 days and followed aquadest treatment for 14 days. At the end of experiment the blood was collected for meansuring SGPT, SGOT and ALP activity. The result showed that treatment of roselle calyx extract had no significant changes in SGPT SGOT, ALP activity. The delayed effect was not also observed. The ethanolic extract of roselle calyx didn’t show toxic effects on the liver on subchronic administration.

  6. Genetic Diversity of Hibiscus tiliaceus (Malvaceae) in China Assessed using AFLP Markers

    Science.gov (United States)

    TANG, TIAN; ZHONG, YANG; JIAN, SHUGUANG; SHI, SUHUA

    2003-01-01

    Amplified fragment length polymorphism (AFLP) markers were used to investigate the genetic variations within and among nine natural populations of Hibiscus tiliaceus in China. DNA from 145 individuals was amplified with eight primer pairs. No polymorphisms were found among the 20 samples of a marginal population of recent origin probably due to a founder effect. Across the other 125 individuals, 501 of 566 bands (88·5 %) were polymorphic, and 125 unique AFLP phenotypes were observed. Estimates of genetic diversity agreed with life history traits of H. tiliaceus and geographical distribution. AMOVA analysis revealed that most genetic diversity resided within populations (84·8 %), which corresponded to results reported for outcrossing plants. The indirect estimate of gene flow based on ϕST was moderate (Nm = 1·395). Long-distance dispersal of floating seeds and local environments may play an important role in shaping the genetic diversity of the population and the genetic structure of this species. PMID:12930729

  7. Antitumoral Effect of Hibiscus sabdariffa on Human Squamous Cell Carcinoma and Multiple Myeloma Cells.

    Science.gov (United States)

    Malacrida, Alessio; Maggioni, Daniele; Cassetti, Arianna; Nicolini, Gabriella; Cavaletti, Guido; Miloso, Mariarosaria

    2016-10-01

    Cancer is a leading cause of death worldwide. Despite therapeutic improvements, some cancers are still untreatable. Recently there has been an increasing interest in the use of natural substances for cancer prevention and treatment. Hibiscus sabdariffa (HS) is a plant, belonging to Malvaceae family, widespread in South Asia and Central Africa. HS extract (HSE) used in folk medicine, gained researchers' interest thanks to its antioxidant, anti-inflammatory, and chemopreventive properties. In the present study, we initially assessed HSE effect on a panel of human tumor cell lines. Then we focused our study on the following that are most sensitive to HSE action cell lines: Multiple Myeloma (MM) cells (RPMI 8226) and Oral Squamous Cell Carcinoma (OSCC) cells (SCC-25). In both RPMI 8226 and SCC-25 cells, HSE impaired cell growth, exerted a reversible cytostatic effect, and reduced cell motility and invasiveness. We evaluated the involvement of MAPKs ERK1/2 and p38 in HSE effects by using specific inhibitors, U0126 and SB203580, respectively. For both SCC-25 and RPMI 8226, HSE cytostatic effect depends on p38 activation, whereas ERK1/2 modulation is crucial for cell motility and invasiveness. Our results suggest that HSE may be a potential therapeutic agent against MM and OSCC.

  8. Boehmenan, a lignan from Hibiscus ficulneus, showed Wnt signal inhibitory activity.

    Science.gov (United States)

    Shono, Takumi; Ishikawa, Naoki; Toume, Kazufumi; Arai, Midori A; Ahmed, Firoj; Sadhu, Samir K; Ishibashi, Masami

    2015-07-15

    The Wnt signal pathway modulates numerous biological processes, and its aberrant activation is related to various diseases. Therefore, inhibition of the Wnt signal may provide an effective (or efficient) strategy for these diseases. Cell-based luciferase assay targeting the Wnt signal (TOP assay) revealed that Hibiscus ficulneus extract inhibited the Wnt signal. The activity-guided isolation of the MeOH extract of H. ficulneus stems yielded four known (1-4) lignans along with myriceric acid (5). Compounds 1-4 potently inhibited the Wnt signal with TOPflash IC50 values of 1.0, 4.5, 6.3, and 1.9 μM, respectively. Compound 1 exhibited cytotoxicity against both Wnt-dependent (HCT116) and Wnt-independent (RKO) cells. Western blot analysis showed that 1 decreased the expression of full, cytosolic and nuclear β-catenin along with c-myc in STF/293 cells. Our results suggested that 1 may have inhibited the Wnt signal by decreasing β-catenin levels. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Hibiscus chlorotic ringspot virus coat protein upregulates sulfur metabolism genes for enhanced pathogen defense.

    Science.gov (United States)

    Gao, Ruimin; Ng, Florence Kai Lin; Liu, Peng; Wong, Sek-Man

    2012-12-01

    In both Hibiscus chlorotic ringspot virus (HCRSV)-infected and HCRSV coat protein (CP) agroinfiltrated plant leaves, we showed that sulfur metabolism pathway related genes-namely, sulfite oxidase (SO), sulfite reductase, and adenosine 5'-phosphosulfate kinase-were upregulated. It led us to examine a plausible relationship between sulfur-enhanced resistance (SED) and HCRSV infection. We broadened an established method to include different concentrations of sulfur (0S, 1S, 2S, and 3S) to correlate them to symptom development of HCRSV-infected plants. We treated plants with glutathione and its inhibitor to verify the SED effect. Disease resistance was induced through elevated glutathione contents during HCRSV infection. The upregulation of SO was related to suppression of symptom development induced by sulfur treatment. In this study, we established that HCRSV-CP interacts with SO which, in turn, triggers SED and leads to enhanced plant resistance. Thus, we have discovered a new function of SO in the SED pathway. This is the first report to demonstrate that the interaction of a viral protein and host protein trigger SED in plants. It will be interesting if such interaction applies generally to other host-pathogen interactions that will lead to enhanced pathogen defense.

  10. Preliminary X-ray Data Analysis of Crystalline Hibiscus Chlorotic Ringspot Virus

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, A.; Speir, J; Yuan, Y; Johnson, J; Wong, S

    2009-01-01

    Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3Synchrotron .2 A resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 . Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV.

  11. Encapsulating anthocyanins from Hibiscus sabdariffa L. calyces by ionic gelation: Pigment stability during storage of microparticles.

    Science.gov (United States)

    de Moura, Sílvia C S R; Berling, Carolina L; Germer, Sílvia P M; Alvim, Izabela D; Hubinger, Míriam D

    2018-02-15

    Hibiscus extract (HE) has a strong antioxidant activity and high anthocyanin content; it can be used as a natural pigment, also adding potential health benefits. The objective of this work was the microencapsulation of HE anthocyanin by ionic gelation (IG) using two techniques: dripping-extrusion and atomization, both by means of a double emulsion (HE/rapseed oil/pectin) and a cross-linked solution (CaCl 2 ). Particles (77-83% moisture content) were conditioned in acidified solution at 5, 15 and 25°C, absence of light, and evaluated for anthocyanins and color for 50-days. The median diameter (D 50 ) of the particles ranged from 78 to 1100μm and encapsulation efficiency ranged from 67.9 to 93.9%. The encapsulation caused higher temperature stability compared with the free extract. The half-life (t 1/2 ) values of the particles ranged from 7 (25°C) to 180days (5°C) for anthocyanins and from 25 (25°C) to 462days (5°C) for Chroma value. The IG increased the stability of HE anthocyanin. Both the dripping-extrusion and the atomization have shown to be feasible techniques. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Accumulation of kaempferitrin and expression of phenyl-propanoid biosynthetic genes in kenaf (Hibiscus cannabinus).

    Science.gov (United States)

    Zhao, Shicheng; Li, Xiaohua; Cho, Dong Ha; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Park, Sang Un

    2014-10-23

    Kenaf (Hibiscus cannabinus) is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL) was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H) and 4-coumarate-CoA ligase (Hc4CL) were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS), chalcone isomerase (HcCHI), and flavone 3-hydroxylase (HcF3H) was highest in young flowers, whereas that of flavone synthase (HcFLS) was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold) in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

  13. Infusion of Hibiscus sabdariffa L. Modulates Oxidative Stress in Patients with Marfan Syndrome

    Science.gov (United States)

    Soto, María Elena; Zuñiga-Muñoz, Alejandra; Guarner Lans, Verónica; Duran-Hernández, Erendira Janet; Pérez-Torres, Israel

    2016-01-01

    Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients. PMID:27413258

  14. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties

    Directory of Open Access Journals (Sweden)

    ASN. Formagio

    Full Text Available The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH. free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g–1, respectively and calyx extracts (474.09 and 148.35 mg g–1, respectively from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL–1 and calyces (37.15 μg mL–1 demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562, with IC50 values of 0.12 mg mL–1 (organosuper® and 1.16 mg mL–1 (poultry litter, with concentration-dependent, cytotoxic and cytocidal effects.

  15. Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products.

    Science.gov (United States)

    Sinela, André; Rawat, Nadirah; Mertz, Christian; Achir, Nawel; Fulcrand, Hélène; Dornier, Manuel

    2017-01-01

    Degradation parameters of two main anthocyanins from roselle extract (Hibiscus sabdariffa L.) stored at different temperatures (4-37°C) over 60days were determined. Anthocyanins and some of their degradation products were monitored and quantified using HPLC-MS and DAD. Degradation of anthocyanins followed first-order kinetics and reaction rate constants (k values), which were obtained by non-linear regression, showed that the degradation rate of delphinidin 3-O-sambubioside was higher than that of cyanidin 3-O-sambubioside with k values of 9.2·10(-7)s(-1) and 8.4·10(-7)s(-1) at 37°C respectively. The temperature dependence of the rate of anthocyanin degradation was modeled by the Arrhenius equation. Degradation of delphinidin 3-O-sambubioside (Ea=90kJmol(-1)) tended to be significantly more sensitive to an increase in temperature than cyanidin 3-O-sambubioside (Ea=80kJmol(-1)). Degradation of these anthocyanins formed scission products (gallic and protocatechuic acids respectively) and was accompanied by an increase in polymeric color index. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves.

    Science.gov (United States)

    Zhen, Jing; Villani, Thomas S; Guo, Yue; Qi, Yadong; Chin, Kit; Pan, Min-Hsiung; Ho, Chi-Tang; Simon, James E; Wu, Qingli

    2016-01-01

    A liquid chromatography-mass spectrometry method was developed for the simultaneous separation, and determination of natural compounds including phenolic acids and flavonoids in the leaves of Hibiscus sabdariffa. By analyzing the UV and MS data, and comparison with authenticated standards, 10 polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and their glycosides were identified together with 5-(hydroxymethyl)furfural. Major constituents in the leaves of 25 different populations from worldwide accessions were quantified and compared with each other. The total phenolic content of each accession was determined using Folin-Ciocalteu assay, ranging from 18.98 ± 2.7 to 29.9 ± 0.5 mg GAE/g. Their in vitro antioxidant activities were measured by ABTS radical cation decolorization assay, varying from 17.5 to 152.5 ± 18.8 μmol Trolox/g. After the treatment of H. sabdariffa leaf extract, the reduction of LPS-induced NO production dose-dependently in RAW 264.7 cell indicates the extract's potential anti-inflammatory activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Anti-inflammatory activities of essential oil isolated from the calyx of Hibiscus sabdariffa L.

    Science.gov (United States)

    Shen, Chun-Yan; Zhang, Tian-Tian; Zhang, Wen-Li; Jiang, Jian-Guo

    2016-10-12

    Hibiscus sabdariffa Linn., belonging to the family of Malvaceae, is considered to be a plant with health care applications in China. The main purpose of this study was to analyze the composition of its essential oil and assess its potential therapeutic effect on anti-inflammatory activity. A water steam distillation method was used to extract the essential oil from H. Sabdariffa. The essential oil components were determined by gas chromatography/mass spectrometry (GC-MS) analysis and a total of 18 volatile constituents were identified, the majority of which were fatty acids and ester compounds. Biological activity showed that the essential oil extracted from H. Sabdariffa exhibited excellent anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. The nitric oxide (NO) inhibition rate reached 67.46% when the concentration of the essential oil was 200 μg mL -1 . Further analysis showed that the anti-inflammatory activity of the essential oil extracted from H. Sabdariffa might be exerted through inhibiting the activation of NF-κB and MAPK (JNK and ERK1/2) signaling pathways to decrease NO and pro-inflammatory cytokine (IL-1, IL-6, TNF-α, COX-2, and iNOS) production. Thus, the essential oil extracted from H. Sabdariffa is a good source of a natural product with a beneficial effect against inflammation, and it may be applied as a food supplement and/or functional ingredient.

  18. Hemidesmus indicus and Hibiscus rosa-sinensis Affect Ischemia Reperfusion Injury in Isolated Rat Hearts

    Directory of Open Access Journals (Sweden)

    Vinoth Kumar Megraj Khandelwal

    2011-01-01

    Full Text Available Hemidesmus indicus (L. R. Br. (HI and Hibiscus rosa-sinensis L. (HRS are widely used traditional medicine. We investigated cardioprotective effects of these plants applied for 15 min at concentrations of 90, 180, and 360 μg/mL in Langendorff-perfused rat hearts prior to 25-min global ischemia/120-min reperfusion (I/R. Functional recovery (left ventricular developed pressure—LVDP, and rate of development of pressure, reperfusion arrhythmias, and infarct size (TTC staining served as the endpoints. A transient increase in LVDP (32%–75% occurred at all concentrations of HI, while coronary flow (CF was significantly increased after HI 180 and 360. Only a moderate increase in LVDP (21% and 55% and a tendency to increase CF was observed at HRS 180 and 360. HI and HRS at 180 and 360 significantly improved postischemic recovery of LVDP. Both the drugs dose-dependently reduced the numbers of ectopic beats and duration of ventricular tachycardia. The size of infarction was significantly decreased by HI 360, while HRS significantly reduced the infarct size at all concentrations in a dose-dependent manner. Thus, it can be concluded that HI might cause vasodilation, positive inotropic effect, and cardioprotection, while HRS might cause these effects at higher concentrations. However, further study is needed to elucidate the exact mechanism of their actions.

  19. Purification, characterization and immunoregulatory activity of a polysaccharide isolated from Hibiscus sabdariffa L.

    Science.gov (United States)

    Zheng, Daheng; Zou, Ye; Cobbina, Samuel Jerry; Wang, Wei; Li, Qian; Chen, Yao; Feng, Weiwei; Zou, Yanmin; Zhao, Ting; Zhang, Min; Yang, Liuqing; Wu, Xiangyang

    2017-03-01

    Hibiscus sabdariffa L. is not only used traditionally as a component of herbal drinks, beverages and flavoring agents but also as a herbal medicine in the drug industry. Bioactive polysaccharides are important constituents of H. sabdariffa that may contribute to the plant's beneficial effects. This study was designed to investigate the structural characteristics of a water-soluble polysaccharide from H. sabdariffa, HSP41, and its immunoregulatory activity on RAW264.7 cells. HSP41 was mainly composed of arabinose, xylose and mannose at a molar ratio of 1:1.34:15.6, with an average molecular weight of 3.3 × 10 5  Da. Fourier transform infrared (FTIR) spectra exhibited absorption peaks characteristic of HSP41. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed the amorphous form and aggregation conformation of HSP41 respectively. HSP41 significantly induced interleukin 1β (IL-1β) and inducible nitric oxide synthase (iNOS) expression in RAW264.7 cells in vitro, promoting an increase in nuclear factor kB p65 (NF-kB p65) levels in the nucleus. The results indicated that HSP41 up-regulated the immune response by stimulating RAW264.7 cell activity. HSP41, a promising immunoregulator, possibly contributes to the health benefits of H. sabdariffa and might have potential applications in health food or medicine. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  20. Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis

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    Philip, Daizy

    2010-03-01

    Biological synthesis of gold and silver nanoparticles of various shapes using the leaf extract of Hibiscus rosa sinensis is reported. This is a simple, cost-effective, stable for long time and reproducible aqueous room temperature synthesis method to obtain a self-assembly of Au and Ag nanoparticles. The size and shape of Au nanoparticles are modulated by varying the ratio of metal salt and extract in the reaction medium. Variation of pH of the reaction medium gives silver nanoparticles of different shapes. The nanoparticles obtained are characterized by UV-vis, transmission electron microscopy (TEM), X-ray diffraction (XRD) and FTIR spectroscopy. Crystalline nature of the nanoparticles in the fcc structure are confirmed by the peaks in the XRD pattern corresponding to (1 1 1), (2 0 0), (2 2 0) and (3 1 1) planes, bright circular spots in the selected area electron diffraction (SAED) and clear lattice fringes in the high-resolution TEM image. From FTIR spectra it is found that the Au nanoparticles are bound to amine groups and the Ag nanoparticles to carboxylate ion groups.

  1. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate.

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    Gheller, Ana Carla Guidini Valentini; Kerkhoff, Jacqueline; Vieira Júnior, Gerardo Magela; de Campos, Kleber Eduardo; Sugui, Marina Mariko

    2017-01-01

    Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

  2. Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacter baumannii

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    Emad Mohamed Abdallah

    2016-11-01

    Full Text Available Objective: To evaluate the antibacterial activity of methanol extract of Hibiscus sabdariffa (H. sabdariffa calyces employed in Sudanese folk medicine against five hospital isolates of multidrug resistant Acinetobacter baumannii (MDR A. baumannii. Methods: The antibacterial activity of 80% methanol extract (v/v of H. sabdariffa calyces was evaluated by agar disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Antibiotic susceptibility of selected A. baumannii strains was tested. Results: In the present investigation, the methanol extract from the calyces of H. sabdariffa exhibited significant antibacterial properties against the non-MDR A. baumannii as well as the MDR A. baumannii strains with a zone of inhibition ranging from (11.3 ± 0.3 to (13.6 ± 0.3 mm. The relative percentage inhibition of H. sabdariffa extract (10 mg/disc with respect to gentamicin (10 mg/disc had potent antibacterial properties and was much more effective than gentamicin. Values of minimum inhibitory concentration and minimum bactericidal concentration ranged from 25 to 50 and 50 to 100 mg/mL, respectively, revealing the potential bactericidal properties of the extract. Conclusions: According to the present study, the calyces of H. sabdariffa can be used as a substitute source of the current ineffective synthetic antibiotics used against MDR A. baumannii.

  3. Preharvest, harvest and postharvest factors inherent to roselle (Hibiscus sabdariffa L. crop: A review.

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    Balois-Morales, R.

    2016-01-01

    Full Text Available Hibiscus sabdariffa L. is cultivated to obtain fiber from the stems, oil from the seeds, and to harvest its calyces. Currently, there is a growing interest for the calyces due to studies that have proven their functional feature as the antioxidant effect, alluded to its anthocyanin content, and other benefits that expand their use to the pharmaceutical sector. However, there are no studies focused on relating all the factors involved in the process, from sowing and preharvest management, to harvest, postharvest management, distribution and the sale of dried calyces. The aim of this review is to highlight the factors involved in Roselle management such as variety, crop nutrition, sowing date, irrigation, plagues and diseases incidence and use of phytohormones. To establish the optimal time of calyces harvest, studies have been focused on determining aspects such as size, pH, dry matter content and anthocyanins at different times after blooming. As for the postharvest, the trend marked by studies is to implement improvements in the drying process of calyces and to let producers, distributors and sellers know the quality standards because much of the damage during postharvest is associated with deficiencies in the way calyces are handled.

  4. Phenolic Content and Antioxidant Activity of Hibiscus cannabinus L. Seed Extracts after Sequential Solvent Extraction

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    Shahid Iqbal

    2012-10-01

    Full Text Available A sequential solvent extraction scheme was employed for the extraction of antioxidant compounds from kenaf (Hibiscus cannabinus L. seeds. Yield of extracts varied widely among the solvents and was the highest for hexane extract (16.6% based on dry weight basis, while water extract exhibited the highest total phenolic content (18.78 mg GAE/g extract, total flavonoid content (2.49 mg RE/g extract, and antioxidant activities (p < 0.05. DPPH and hydroxyl radical scavenging, β-carotene bleaching, metal chelating activity, ferric thiocyanate and thiobarbituric acid reactive substances assays were employed to comprehensively assess the antioxidant potential of different solvent extracts prepared sequentially. Besides water, methanolic extract also exhibited high retardation towards the formation of hydroperoxides and thiobarbituric acid reactive substances in the total antioxidant activity tests (p < 0.05. As conclusion, water and methanol extracts of kenaf seed may potentially serve as new sources of antioxidants for food and nutraceutical applications.

  5. Ultrasonic-assisted extraction and in-vitro antioxidant activity of polysaccharide from Hibiscus leaf.

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    Afshari, Kasra; Samavati, Vahid; Shahidi, Seyed-Ahmad

    2015-03-01

    The effects of ultrasonic power, extraction time, extraction temperature, and the water-to-raw material ratio on extraction yield of crude polysaccharide from the leaf of Hibiscus rosa-sinensis (HRLP) were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize HRLP extraction yield by implementing the Box-Behnken design (BBD). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). Analysis of the results showed that the linear and quadratic terms of these four variables had significant effects. The optimal conditions for the highest extraction yield of HRLP were: ultrasonic power, 93.59 W; extraction time, 25.71 min; extraction temperature, 93.18°C; and the water to raw material ratio, 24.3 mL/g. Under these conditions, the experimental yield was 9.66±0.18%, which is well in close agreement with the value predicted by the model 9.526%. The results demonstrated that HRLP had strong scavenging activities in vitro on DPPH and hydroxyl radicals. Copyright © 2014 Elsevier B.V. All rights reserved.

  6. Accumulation of Kaempferitrin and Expression of Phenyl-Propanoid Biosynthetic Genes in Kenaf (Hibiscus cannabinus

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    Shicheng Zhao

    2014-10-01

    Full Text Available Kenaf (Hibiscus cannabinus is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H and 4-coumarate-CoA ligase (Hc4CL were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS, chalcone isomerase (HcCHI, and flavone 3-hydroxylase (HcF3H was highest in young flowers, whereas that of flavone synthase (HcFLS was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

  7. Morphological characteristics and pathogenicity of fungi associated with Roselle (Hibiscus Sabdariffa) diseases in Penang, Malaysia.

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    Eslaminejad, Touba; Zakaria, Maziah

    2011-11-01

    Roselle, or Jamaica sorrel (Hibiscus sabdariffa) is a popular vegetable in many tropical regions, cultivated for its leaves, seeds, stems and calyces which, the dried calyces are used to prepare tea, syrup, jams and jellies and as beverages. The main objectives of this study were to identify and characterise fungal pathogens associated with Roselle diseases based on their morphological and cultural characteristics and to determine the pathogenicity of four fungi infecting Roselle seedlings, namely Phoma exigua, Fusarium nygamai, Fusarium tgcq and Rhizoctonia solani in Penang. A total of 200 fungal isolates were obtained from 90 samples of symptomatic Roselle tissues. The isolates were identified based on cultural and morphological characteristics, as well as their pathogenicity. The fungal pathogen most frequently isolated was P. exigua (present in 45% of the samples), followed by F. nygamai (25%), Rhizoctonia solani (19%) and F. camptoceras (11%). Pathogenicity tests showed that P. exigua, F. nygamai, F. camptoceras and R. solani were able to infect both wounded and unwounded seedlings with different degrees of severity as indicated by the Disease severity (DS). R. solani was the most pathogenic fungus affecting both wounded and unwounded Roselle seedlings, followed by P. exigua that was highly pathogenic on wounded seedlings. F. nygamai was less pathogenic while the least pathogenic fungus was F. camptoceras, infecting only the unwounded seedlings but, surprisingly, not the wounded plants. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury

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    Bakhtiari, Elham; Hosseini, Azar; Mousavi, Seyed Hadi

    2015-01-01

    Objectives: Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdariffa exert pharmacological actions such as potent antioxidant. Therefore, in this study we investigated the protective effect of extract of H. sabdariffa against SGD-induced PC12 cells injury. Materials and Methods: Cells were pretreated with different concentrations of H. sabdariffa extract (HSE) for 2 hr, and then exposed to SGD condition for 6, 12 and 18 hr. Results: SGD caused a major reduction in cell viability after 6, 12, and 18 hr as compared with control cells (psabdariffa has the potential to be used as a new therapeutic approach for neurodegenerative disorders. PMID:26101756

  9. Antipyretic activity of the extracts of Hibiscus sabdariffa calyces L. in experimental animals

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    Wantana Reanmongkol

    2007-03-01

    Full Text Available The effects of the extracts from Hibiscus sabdariffa calyces L. (H. sabdariffa on nociceptive response using writhing, hot plate and formalin test in mice and the antipyretic activity in yeast-induced fever in rats, were examined. Anti-inflammatory activity was also investigated on carrageenin-induced paw edema in rats. No acute toxicity was observed in mice after oral administration of the ethanol and aqueous extract of H. sabdariffa calyces at the dose of 15 g/kg. Oral administration of the ethanol extract at the dose of 800 mg/kg significantly decreased the number of contortions and stretchings induced by acetic acid in mice. The aqueous extracts had no effect on this test. Neither the ethanol nor aqueous extract had an effect in the formalin and hot plate tests in mice. The ethanol and the vacuum dried extract of H. sabdariffa calyces (200-800 mg/kg, p.o. decreased the yeast-induced fever in rats. The H. sabdariffa extract had no effect on carrageenininduced paw edema in rats. These results suggest that the ethanol and aqueous extract (vacuum dry of H. sabdariffa calyces possess antipyretic action through mechanisms that are different from that of aspirin.

  10. Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. seeds

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    Sumaia Awad Elkariem Ali

    2014-06-01

    Full Text Available Hibiscus (H. sabdariffa (commonly known as “Karkadeh” in Arabic is widely used in various pharmacological applications in Sudan. The present study was carried out to investigate the anti-inflammatory and analgesic activities of H. sabdariffa seed extracts using rat models. In acute anti-inflammatory models, oral administration of petroleum ether extract of H. sabdariffa seeds inhibited the hind paw edema (p<0.01 which was induced by carrageenan. The petroleum ether extract exhibited significant (p<0.01 inhibition of vascular permeability in rats induced by intraperitoneal injection of acetic acid (0.6%. In cotton pellet granuloma method, the petroleum ether extract of H. sabdariffa seed showed significant inhibition of granuloma. The extract reduced (p<0.001 abdominal constrictions which was induced by injection of acetic acid (0.7%. Analysis of seed oil of H. sabdariffa using Gas Chromatography revealed the presence of three fatty acids; these were linolelaidic acid, arachidic acid, and palmitic acid. In conclusion, H. sabdariffa seeds possess anti-inflammatory and analgesic activities in rat model.

  11. Infusion of Hibiscus sabdariffa L. Modulates Oxidative Stress in Patients with Marfan Syndrome.

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    Soto, María Elena; Zuñiga-Muñoz, Alejandra; Guarner Lans, Verónica; Duran-Hernández, Erendira Janet; Pérez-Torres, Israel

    2016-01-01

    Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients.

  12. Safety assessment of Hibiscus sabdariffa after maternal exposure on male reproductive parameters in rats.

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    de Arruda, Aline; Cardoso, Claudia Andrea L; Vieira, Maria do Carmo; Arena, Arielle Cristina

    2016-01-01

    Hibiscus sabdariffa L. (Malvaceae) is a species widely used in folk medicine for the treatment of some disorders. This study evaluated the effects of H. sabdariffa (HS) on the development of the male reproductive tract in rats following in utero exposure. Pregnant rats received 250 or 500 mg/kg of HS extract or vehicle from gestational day 12 until day 21 of lactation. Both doses of HS increased the body weight of male offspring at weaning, without compromising the puberty onset parameters. At puberty, there was a significant increase in the vas deferens absolute weight and a significant reduction in the relative weight of kidney at higher dose. These animals also presented a significant reduction in the sperm number in the caput/corpus of epididymis after exposure to both doses and a reduction in the sperm number in the cauda epididymis for the lower dose. At adulthood, the highest dose significantly reduced the sperm production in relation to controls and both doses provoked a reduction in the relative sperm number in the epididymis without affecting the sperm morphology. These findings demonstrated that maternal exposure to H. sabdariffa can adversely influence the male reproductive system in rats.

  13. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties.

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    Formagio, A S N; Ramos, D D; Vieira, M C; Ramalho, S R; Silva, M M; Zárate, N A H; Foglio, M A; Carvalho, J E

    2015-01-01

    The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle) cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH.) free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g-1, respectively) and calyx extracts (474.09 and 148.35 mg g-1, respectively) from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL-1) and calyces (37.15 μg mL-1) demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562), with IC50 values of 0.12 mg mL-1 (organosuper®) and 1.16 mg mL-1 (poultry litter), with concentration-dependent, cytotoxic and cytocidal effects.

  14. Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa

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    Loo, Shining; Kam, Antony; Xiao, Tianshu; Nguyen, Giang K. T.; Liu, Chuan Fa; Tam, James P.

    2016-01-01

    Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor against porcine pancreatic elastase was first identified from the squash family in 1989. Here, we report the identification and characterization of a knottin-type human neutrophil elastase inhibitor from Hibiscus sabdariffa of the Malvaceae family. Combining proteomic and transcriptomic methods, we identified a panel of novel cysteine-rich peptides, roseltides (rT1-rT8), which range from 27 to 39 residues with six conserved cysteine residues. The 27-residue roseltide rT1 contains a cysteine spacing and amino acid sequence that is different from the squash knottin-type elastase inhibitor. NMR analysis demonstrated that roseltide rT1 adopts a cystine-knot fold. Transcriptome analyses suggested that roseltides are bioprocessed by asparagine endopeptidases from a three-domain precursor. The cystine-knot structure of roseltide rT1 confers its high resistance against degradation by endopeptidases, 0.2 N HCl, and human serum. Roseltide rT1 was shown to inhibit human neutrophil elastase using enzymatic and pull-down assays. Additionally, roseltide rT1 ameliorates neutrophil elastase-stimulated cAMP accumulation in vitro. Taken together, our findings demonstrate that roseltide rT1 is a novel knottin-type neutrophil elastase inhibitor with therapeutic potential for neutrophil elastase associated diseases. PMID:27991569

  15. Effect of Hibiscus sabdariffa and its anthocyanins on some reproductive aspects in rats.

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    Ali, Badreldin H; Al-Lawati, Intisar; Beegam, Sumyia; Ziada, Amal; Al Salam, Suhail; Nemmar, Abderrahim; Blunden, Gerald

    2012-01-01

    An aqueous extract of Hibiscus sabdariffa L. is a common beverage in many parts of the world. Reports on its effect on reproduction are conflicting, with anecdotal evidence that the plant is an aphrodisiac, while others report that it is estrogenic, and adversely affects spermatogenesis in rats. We have studied the effect of different concentrations of aqueous extracts of H. sabdariffa calyces (10%, 15% and 20%) used as drinking water for 10 consecutive weeks, and its anthocyanins (50, 100, 200 mg/kg for 5 days, orally) on the weight and histology of the testis, and on some biochemical constituents in testicular homogenates, in addition to the plasma concentrations of testosterone, luteinizing hormone and estradiol. The possible presence of an estrogenic effect of the extract and anthocyanins on the uteri of immature female rats was also tested. Neither the H. sabdariffa extract nor the anthocyanins significantly altered either testicular weight and histology, or uterus weight. Plasma concentrations of the three hormones studied, the testicular concentrations of protein, reduced glutathione and total cholesterol, and superoxide dismutase activity were all insignificantly affected by either the extract or the anthocyanins, except for a slight, but statistically significant, decrease in testicular protein concentration caused by the 15% aqueous extract when compared with controls. These results suggest that H. sabdariffa exerts no adverse effect on the male reproductive system. Consumption of H. sabdariffa aqueous extract inhibited the growth of the rats compared with the controls.

  16. Infusion of Hibiscus sabdariffa L. Modulates Oxidative Stress in Patients with Marfan Syndrome

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    María Elena Soto

    2016-01-01

    Full Text Available Marfan syndrome (MFS is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD, glutathione peroxidase (GPx, glutathione-S-transferase (GST, glutathione reductase (GSSG-R, glutathione (GSH, lipid peroxidation (LPO index, total antioxidant capacity (TAC, and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p=0.03, EGPx (p=0.04, GST (p=0.03, GSH (p=0.01, and TAC and ascorbic acid (p=0.02 but GSSG-R activity (p=0.04 and LPO (p=0.02 were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients.

  17. Hibiscus sabdariffa extractivities on cadmium-mediated alterations of human U937 cell viability and activation.

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    Okoko, Tebekeme; Ere, Diepreye

    2012-01-01

    To investigate the effect of the anthocyanin-rich extract of Hibiscus sabdariffa (H. sabdariffa) calyx on the viability of cadmium-treated U937 cells and cadmium-mediated activation of U937-derived macrophages. The macrophage cell line U937 was treated with cadmium (0.1 μ mol/L) and later incubated with the anthocyanin-rich extract and cell viability was assessed via trypan blue staining. In the other experiment, the U937 cells were transformed to the macrophage form by treatment with phorbol 12, myristate 13, and acetate and incubated with cadmium (10 μ mol/L). The anthocyanin-rich extract was added to the cells later and subsequently, the supernatant of each cell culture was analysed for the production of tumour necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), nitric oxide, and catalase activity as indices for the activation of macrophages. It revealed that the anthocynanin-rich extract significantly (P sabdariffa possesses significant immunoprotective effect. These corroborate the immense reported antioxidant and medicinal potential of the calyces of the plant which could be exploited for pharmacological and neutraceutical advantages. Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  18. Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine

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    Abdallah, Emad Mohamed

    2016-01-01

    Background: Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine. Materials and Methods: The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods. Results: The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect. Conclusion: The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents. PMID:27104041

  19. Hibiscus sabdariffa extract inhibits obesity and fat accumulation, and improves liver steatosis in humans.

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    Chang, Hong-Chou; Peng, Chiung-Huei; Yeh, Da-Ming; Kao, Erl-Shyh; Wang, Chau-Jong

    2014-04-01

    Obesity is associated with a great diversity of diseases including non-alcoholic fatty liver disease. Our previous report suggested that Hibiscus sabdariffa extracts (HSE) had a metabolic-regulating and liver-protecting potential. In this study, we performed a clinical trial to further confirm the effect of HSE. Subjects with a BMI ≧ 27 and aged 18-65, were randomly divided into control (n = 17) and HSE-treated (n = 19) groups, respectively, for 12 weeks. Our data showed that consumption of HSE reduced body weight, BMI, body fat and the waist-to-hip ratio. Serum free fatty acid (FFA) was lowered by HSE. Anatomic changes revealed that HSE improved the illness of liver steatosis. Ingestion of HSE was well tolerated and there was no adverse effect during the trial. No alteration was found for serum α-amylase and lipase. The clinical effect should mainly be attributed to the polyphenols of HSE, since composition analysis showed that branched chain-amino acids, which is associated with obesity, is not obviously high. In conclusion, consumption of HSE reduced obesity, abdominal fat, serum FFA and improved liver steatosis. HSE could act as an adjuvant for preventing obesity and non-alcoholic fatty liver.

  20. Analisis Efisiensi Pada Sistem Pengeringan Bunga Rosella (Hibiscus sabdariffa L Menggunakan Alat Pengering Tipe Lemari

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    Refli Safrizal

    2012-10-01

    Full Text Available Efficiency Analysis in Drying System for Rosella (Hibiscus sabdariffa L Using Cabinet Type Dryer ABSTRACT. Drying is one of the most important process in producing a good quality of rosella tea. The study was aimed to evaluate the drying efficiency of rosella tea by using a cabinet dryer. The source of energy for the dryer was from the burning of charcoal. The charcoal was prepared from the coconut shell since it was abundant biomass product. About 7.5 kg of fresh rosella flower was dried for 17 hours. The total of charcoal consumed was 17 kg. The moisture content of rosella flower was reduced from 90 to 12 %. The analysis showed that the total of energy produced from the burning of 17 kg charcoal was 309.4 MJ, whilst the total energy needed to dry the flower was 15,72 MJ. Therefore, the drying efficiency was still low about 4.58%. Further study is recommended to improve the drying efficiency for example by the use of deeper depth of dried products between 2 and 6 cm as well as by improving the system of hot air circulation.

  1. Toxicity studies of the water extract from the calyces of Hibiscus sabdariffa L. in rats.

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    Sireeratawong, Seewaboon; Itharat, Arunporn; Khonsung, Parirat; Lertprasertsuke, Nirush; Jaijoy, Kanjana

    2013-01-01

    Acute and chronic toxicities of the water extract from calyces of Hibiscus sabdariffa were studied in male and female rats. After 14 days of a single oral administration of test substance 5,000 mg/kg body weight, measurement of the body and organ weights, necropsy and health monitoring were performed. No signs and differences of the weights or behaviour compared to the control rats were observed. The results indicated that the single oral administration of H. sabdariffa extract in the amount of 5,000 mg/kg body weight does not produce acute toxicity. The chronic toxicity was determined by oral feeding both male and female rats daily with the extract at the doses of 50, 100, and 200 mg/kg body weight for 270 days. The examinations of signs, animal behaviour and health monitoring showed no defects in the test groups compared to the control groups. Both test and control groups (day 270th) and satellite group (day 298th) were analysed by measuring their final body and organ weights, taking necropsy, and examining haematology, blood clinical chemistry, and microanatomy. Results showed no differences from the control groups. Overall, our study demonstrated that an oral administration of H. sabdariffa extract at the doses of 50, 100 and 200 mg/kg body weight for 270 days does not cause chronic toxicity in rat.

  2. Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract.

    Science.gov (United States)

    Laikangbam, Reena; Damayanti Devi, M

    2012-06-01

    The present study aims at systematic evaluation of the calyces of Hibiscus sabdariffa to establish its scientific validity for anti-urolithiatic property using ethylene glycol-induced hyperoxaluria model in male albino rats. Administration of a mixture of 0.75% ethylene glycol and 2% ammonium chloride resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. The decrease in the serum calcium concentration indicates an increased calcium oxalate formation. Supplementation of aqueous extract of H. sabdariffa at different doses (250, 500 and 750 mg/kg body weight) significantly lowered the deposition of stone-forming constituents in the kidneys and serum of urolithiatic rats. These findings have been confirmed through histological investigations. Results of in vivo genotoxicity testing showed no significant chromosomal aberrations in the bone marrow cells of ethylene glycol-induced rats. The plant extracts at the doses investigated induced neither toxic nor lethal effects and are safe. It can be concluded that the calyces of H. sabdariffa are endowed with anti-urolithiatic activity and do not have genotoxic effects. Thus, it can be introduced in clinical practices and medicine in the form of orally administered syrup after further investigations and clinical trials.

  3. Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents.

    Science.gov (United States)

    Jabeur, Inès; Pereira, Eliana; Barros, Lillian; Calhelha, Ricardo C; Soković, Marina; Oliveira, M Beatriz P P; Ferreira, Isabel C F R

    2017-10-01

    The nutritional and bioactive composition of plants have aroused much interest not only among scientists, but also in people's daily lives. Apart from the health benefits, plants are a source of pigments that can be used as natural food colorants. In this work, the nutritional composition of Hibiscus sabdariffa L. was analysed, as well as its bioactive compounds and natural pigments. Glucose (sugar), malic acid (organic acid), α-tocopherol (tocopherol) and linoleic acid (fatty acid) were the major constituents in the corresponding classes. 5-(Hydroxymethyl) furfural was the most abundant non-anthocyanin compound, while delphinidin-3-O-sambubioside was the major anthocyanin both in its hydroethanolic extract and infusion. H. sabdariffa extracts showed antioxidant and antimicrobial activities, highlighting that the hydroethanol extract presents not only lipid peroxidation inhibition capacity, but also bactericidal/fungicidal inhibition ability for all the bacteria and fungi tested. Furthermore, both extracts revealed the absence of toxicity using porcine primary liver cells. The studied plant species was thus not only interesting for nutritional purposes but also for bioactive and colouring applications in food, cosmetic and pharmaceutical industries. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Determination of antioxidant activity of Hibiscus sabdariffa and Croton caudatus in Saccharomyces cerevisiae model system.

    Science.gov (United States)

    Subhaswaraj, Pattnaik; Sowmya, M; Bhavana, V; Dyavaiah, Madhu; Siddhardha, Busi

    2017-08-01

    From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC 50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL. The ethanolic leaf extract of H. sabdariffa and C. caudatus also showed significant hydoxyl radical scavenging and total antioxidant activity. Ascorbic acid was used as positive control. The in vitro antioxidant activity was further supported by in vivo studies using radical scavenging mechanism in S. cerevisiae wild type and its isogenic deletion strains sod1∆ and tsa1∆ . The mutant yeast cells substantially scavenged the stress generated by H 2 O 2 when supplemented with ethanolic leaf extract of H. sabdariffa and C. caudatus as evident from spot assays followed by fluorescence assay (DCF-DA) using fluorescence microscopic and intensity studies. H. sabdariffa and C.caudatus significantly neutralize the ROS level in yeast mutants with concomitant decrease in fluorescence intensity as compared to the untreated yeast cells. The results suggested the efficacy of H. sabdariffa and C. caudatus as potent antioxidants in yeast system and thus their futuristic applications in therapeutics.

  5. Microbial Quality and Proximate Composition of Dried Hibiscus sabdariffa Calyxes in Uyo, Eastern Nigeria

    Directory of Open Access Journals (Sweden)

    Adebayo-tayo, B. C.

    2009-01-01

    Full Text Available Dried Hibiscus sabdarifa calyxes collected from different markets in Uyo, Eastern Nigeria were evaluated for microbial quality, Aflatoxin contamination and proximate composition. The results showed that all the calyxes were contaminated with microorganism. The total bacteria count ranged from 5.0 x 10^3 to 8.1 x 10^4 cfu/g in which the highest count was obtained from dried calyxes from Itam Market. Coliform were not detected in most of the sample except samples from Uyo main market and Ikot Ekpene market in which the coliform level is below the acceptable limit. Salmonella/Shigella was not detected in the sample. The fungi count ranged from 3.4 x 10^4 to 7.3 x 10^4. The associated bacteria were Staphylococcus aureus, Bacillus subtilis, Bacillus sp. Enterococuss faecalis, Micrococcus sp. and Klebsiella sp. The associated fungi were Aspergillus flavus, A. terreus, A. glaucus, Penicillium citrinum, Fusarium oxysporum, Rhizopus sp. and Mucor sp. A. glaucus had the highest frequency of occurrence among the isolated fungi. Out of the sample obtained Aflatoxin B1 was detected in two samples and it ranged from 1.57 to 17.8 /µg/kg. The proximate analysis revealed that the crude protein ranged from 8.34 – 9.97%, crude fibre (7.26 – 7.82% and fat (8.51 – 9.26%. The moisture content ranged from 13.13 – 14.85%.

  6. Polyphenols of Hibiscus sabdariffa improved diabetic nephropathy via attenuating renal epithelial mesenchymal transition.

    Science.gov (United States)

    Yang, Yi-Sun; Wang, Chau-Jong; Huang, Chien-Ning; Chen, Mu-Lin; Chen, Ming-Jinn; Peng, Chiung-Huei

    2013-08-07

    We previously reported that Hibiscus sabdariffa polyphenol extracts (HPE) are beneficial for diabetic nephropathy. Since an epithelial to mesenchymal transition (EMT) is critical in renal fibrosis, the present study aimed to investigate whether HPE could prevent EMT of tubular cells. Treatment of HPE reduced angiotensin II receptors (AT)-1 and transforming growth factor β1 (TGF-β1) evoked by high glucose and recovered the increased vimentin and decreased E-cadherin. HPE decreased fibronectin, thus avoiding EMT and accompanying fibrosis. AT-1 was upstream to TGF-β1, while there were recruitment signals between AT-1 and TGF-β1. Scan electron microscopy (SEM) and immunohistochemistry (IHC) revealed that the interacting filaments of tubular cells disappeared when treated with high glucose, and type IV collagen of tubulointerstitial decreased in diabetic kidneys. Treatment of HPE recovered morphological changes of cell junction and basement membrane. We suggest that HPE has the potential to be an adjuvant for diabetic nephropathy by regulating AT-1/TGF-β1 and EMT.

  7. Occurrence of Root Rot and Vascular Wilt Diseases in Roselle (Hibiscus sabdariffa L.) in Upper Egypt

    Science.gov (United States)

    Hassan, Naglaa; Shimizu, Masafumi

    2014-01-01

    Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt. PMID:24808737

  8. Efficacy of Hibiscus sabdariffa and Telfairia occidentalis in the attenuation of CCl4-mediated oxidative stress.

    Science.gov (United States)

    Victor, Ikpeme Ekei; Ugorji, Udensi Ogbuagu; Adeyinka, Adetayo

    2014-09-01

    To assess the efficacy of Hibiscus sabdariffa (H. sabdariffa) and Telfairia occidentalis (T. occidentalis) extracts in the attenuation of CCl4-mediated oxidative stress. Seventy-two healthy matured male albino rats weighing between (120±20) g were divided into 6 groups (A-F) in a 2×6 factorial experiment using completely randomized design. Rats in Group A received only water, B received 1 mL/kg CCl4, C received 300 mg/kg, D received 600 mg/kg, E received CCl4+300 mg/kg while F received CCl4+600 mg/kg for each of the extract, respectively. Semen from epididymes was obtained for sperm analysis while blood was obtained through cardiac puncture for biochemical analysis, after the treatment regimen. Our results showed that CCl4 induction elevated aspartate aminotransferase, alanine transaminase, alkaline phosphatase, total protein, globulin levels significantly (Psabdariffa and T. occidentalis extracts at the doses of 300 and 600 mg/kg caused the reversal of these effects significantly. Implicitly, the implication of our results is that H. sabdariffa and T. occidentalis extracts might be ted and optimized for the management of oxidative stress-related organ injuries, including infertility, though further researches are needed. Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  9. Hibiscus rosa- sinensis leaf extract as coagulant aid in leachate treatment

    Science.gov (United States)

    Awang, Nik Azimatolakma; Aziz, Hamidi Abdul

    2012-12-01

    Hibiscus rosa- sinensis is a biodegradable material that has remained untested for flocculating properties. The objective of this study is to examine the efficiency of coagulation-flocculation processes for the removal of color, iron (Fe3+), suspended solids, turbidity and ammonia nitrogen(NH3-N), from landfill leachate using 4,000 mg/L alum in conjunction with H. rosa- sinensis leaf extract (HBaqs). Hydroxyl (O-H) and (carboxyl) C=O functional groups along the HBaqs chain help to indulge flocculating efficiency of HBaqs via bridging. The experiments confirm the positive coagulation properties of HBaqs. The Fe3+ removal rate using 4,000 mg/L alum as sole coagulant was approximately 60 %, and increased to 100 % when 4,000 mg/L alum was mixed with 500 mg/L HBaqs. By mixing, 4,000 mg/L alum with 100-500 mg/L HBaqs, 72 % of SS was removed as compared with only 45 % reduction using 4,000 mg/L alum as sole coagulant.

  10. Changes in protein patterns and in vivo protein synthesis during senescence of hibiscus petals

    International Nuclear Information System (INIS)

    Woodson, W.R.; Handa, A.K.

    1986-01-01

    Changes in proteins associated with senescence of the flowers of Hibiscus rosa-sinensis was studied using SDS-PAGE. Total extractable protein from petals decreased with senescence. Changes were noted in patterns of proteins from aging petals. Flower opening and senescence was associated with appearance and disappearance of several polypeptides. One new polypeptide with an apparent mw of 41 kd was first seen the day of flower opening and increased to over 9% of the total protein content of senescent petal tissue. Protein synthesis during aging was investigated by following uptake and incorporation of 3 H-leucine into TCA-insoluble fraction of petal discs. Protein synthesis, as evidenced by the percent of label incorporated into the TCA-insoluble fraction, was greatest (32%) the day before flower opening. Senescent petal tissue incorporated 4% of label taken up into protein. Proteins were separated by SDS-PAGE and labelled polypeptides identified by fluorography. In presenescent petal tissue, radioactivity was distributed among several major polypeptides. In senescent tissue, much of the radioactivity was concentrated in the 41 kd polypeptide

  11. Molecular Characterization of Ferulate 5-Hydroxylase Gene from Kenaf (Hibiscus cannabinus L.

    Directory of Open Access Journals (Sweden)

    Jonggeun Kim

    2013-01-01

    Full Text Available The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.. Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monooxygenase (CYP84, is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF encoding 518 amino acids (GenBank Accession number JX524278. The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78% with that of Populus trichocarpa. Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old of stem tissues, with a certain level of expression in all tissues tested. The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h, wounding (24 h, cold (24 h, abscisic acid (24 h, and methyl jasmonate (24 h.

  12. Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces.

    Science.gov (United States)

    Ajay, M; Chai, H J; Mustafa, A M; Gilani, A H; Mustafa, M R

    2007-02-12

    Previous studies have demonstrated the anti-hypertensive effects of Hibiscus sabdariffa L. (HS) in both humans and experimental animals. To explore the mechanisms of the anti-hypertensive effect of the HS, we examined the effects of a crude methanolic extract of the calyces of HS (HSE) on vascular reactivity in isolated aortas from spontaneously hypertensive rats. HSE relaxed, concentration-dependently, KCl (high K(+), 80 mM)- and phenylephrine (PE, 1 microM)-pre-contracted aortic rings, with a greater potency against the alpha(1)-adrenergic receptor agonist. The relaxant effect of HSE was partly dependent on the presence of a functional endothelium as the action was significantly reduced in endothelium-denuded aortic rings. Pretreatment with atropine (1 microM), L-NAME (10 microM) or methylene blue (10 microM), but not indomethacin (10 microM), significantly blocked the relaxant effects of HSE. Endothelium-dependent and -independent relaxations induced by acetylcholine and sodium nitroprusside, respectively, were significantly enhanced in aortic rings pretreated with HSE when compared to those observed in control aortic rings. The present results demonstrated that HSE has a vasodilator effect in the isolated aortic rings of hypertensive rats. These effects are probably mediated through the endothelium-derived nitric oxide-cGMP-relaxant pathway and inhibition of calcium (Ca(2+))-influx into vascular smooth muscle cells. The present data further supports previous in vivo findings and the traditional use of HS as an anti-hypertensive agent.

  13. Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits.

    Science.gov (United States)

    Chen, Chang-Che; Hsu, Jeng-Dong; Wang, San-Fa; Chiang, Huei-Ching; Yang, Mon-Yuan; Kao, Erl-Shyh; Ho, Yung-Chyan; Wang, Chau-Jong

    2003-08-27

    Hibiscus sabdariffa L., a local soft drink material and medicinal herb, is usually used effectively in native medicines against hypertension, pyrexia, and liver disorders. Here, we report an extract, HSE (H. sabdariffa extract), which is designed to exhibit hypolipidemia and antiatherosclerotic effects in rabbits with experimental atherosclerosis. New Zealand White rabbits were fed with a normal diet, high cholesterol (1.3%), lard oil (3%) diet (HCD) with or without 0.5 or 1% HSE for 10 weeks. The levels of triglyceride, cholesterol, and low-density lipoprotein cholesterol (LDL-C) were lower in the serum of rabbits fed HCD plus HSE than in the serum of rabbits fed HCD. Feeding HSE (0.5 and 1% in the diet) to rabbits significantly reduced severe atherosclerosis in the aorta. Histopathological examination showed that HSE reduced foam cell formation and inhibited smooth muscle cell migration and calcification in the blood vessel of rabbits. These results suggest that HSE inhibits serum lipids and shows an antiatherosclerotic activity.

  14. Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa Linn. leaves and calyces extracts in rats.

    Science.gov (United States)

    Ochani, Pooja C; D'Mello, Priscilla

    2009-04-01

    Antioxidant and antihyperlipidemic activities of the extracts of leaves and calyces of Hibiscus sabdariffa were investigated by studying their in vitro inhibitory activity on lipid peroxidation and in vivo effects on cholesterol induced hyperlipidemia. Highest antioxidant activity was exhibited by ethanolic extract of calyces followed by ethanolic extract of leaves followed by aqueous extract of leaves of H. sabdariffa. In cholesterol induced hyperlipidemic model, groups of rats treated with extracts of calyces and leaves of H. sabdariffa showed a significant decrease in the serum TC, LDL-C, VLDL-C, TAG values alongwith an increase in serum HDL-C levels. The treated groups also showed significant decrease in the atherogenic index, LDL-C: HDL-C risk ratios, and in the levels of SGOT, SGPT and ALP activities compared to cholesterol induced hyperlipidemic control group. Significant antihyperlipidemic activity was shown by ethanolic extract of calyces, followed by ethanolic extract of leaves. It was observed from the histopathological findings that rats fed with H. sabdariffa extracts showed decrease in granular degeneration caused by cholesterol feedings. Results suggest that the ethanolic extracts of calyces and leaves of H. sabdarifa containing polyphenols and flavanols possess significant antioxidant and antihyperlipidemic activities.

  15. Phytoremediation of copper and zinc in sewage sludge amended soils using jatropha curcas and hibiscus cannabinus

    International Nuclear Information System (INIS)

    Aishah, R.M.; Shamshuddin, J.; Fauziah, C.I.

    2016-01-01

    Phytoremediation can be potentially used to remediate heavy metal contaminated soils. A glasshouse experiment was conducted to determine the extent of Jatropha curcas and Hibiscus cannabinus efficiency to the remediation of zinc and copper contaminated soils amended with sewage sludge. An Oxisol (Munchong Series) and an Ultisol (Bungor Series) were used in this experiment, which was laid out using a randomized completely block design in six replication. The plants in pots having soil containing 0, 5 and 10% (w/w) sewage sludge were grown for six months. Phytoremediation can take place successfully as shown by the decrease of total Zn and Cu in the treated soils, where the concentrations of Zn and Cu in the tested soils were higher before planting as compared to after planting. Most of the Zn and Cu taken up by the tested plants were stored in the shoots (leaves+ stem). The fractionation of Zn and Cu in sewage sludge, untreated and treated soils was studied before and after planting. The results of the fractionation study showed that the dominant Zn and Cu in the soil were in their residual form. At harvest, the percentages of water soluble and exchangeable fraction were increased, implying that some of the residual fraction may have changed to other forms. In general, there was no significant difference between the different metal fractions in the Oxisol and Ultisol. (author)

  16. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

    Directory of Open Access Journals (Sweden)

    Ana Carla Guidini Valentini Gheller

    2017-01-01

    Full Text Available Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

  17. Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa.

    Science.gov (United States)

    Loo, Shining; Kam, Antony; Xiao, Tianshu; Nguyen, Giang K T; Liu, Chuan Fa; Tam, James P

    2016-12-19

    Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor against porcine pancreatic elastase was first identified from the squash family in 1989. Here, we report the identification and characterization of a knottin-type human neutrophil elastase inhibitor from Hibiscus sabdariffa of the Malvaceae family. Combining proteomic and transcriptomic methods, we identified a panel of novel cysteine-rich peptides, roseltides (rT1-rT8), which range from 27 to 39 residues with six conserved cysteine residues. The 27-residue roseltide rT1 contains a cysteine spacing and amino acid sequence that is different from the squash knottin-type elastase inhibitor. NMR analysis demonstrated that roseltide rT1 adopts a cystine-knot fold. Transcriptome analyses suggested that roseltides are bioprocessed by asparagine endopeptidases from a three-domain precursor. The cystine-knot structure of roseltide rT1 confers its high resistance against degradation by endopeptidases, 0.2 N HCl, and human serum. Roseltide rT1 was shown to inhibit human neutrophil elastase using enzymatic and pull-down assays. Additionally, roseltide rT1 ameliorates neutrophil elastase-stimulated cAMP accumulation in vitro. Taken together, our findings demonstrate that roseltide rT1 is a novel knottin-type neutrophil elastase inhibitor with therapeutic potential for neutrophil elastase associated diseases.

  18. Germination biology of Hibiscus tridactylites in Australia and the implications for weed management

    Science.gov (United States)

    Chauhan, Bhagirath Singh

    2016-05-01

    Hibiscus tridactylites is a problematic broadleaf weed in many crops in Australia; however, very limited information is available on seed germination biology of Australian populations. Experiments were conducted to evaluate the effect of environmental factors on germination and emergence of H. tridactylites. Germination was stimulated by seed scarification, suggesting the inhibition of germination in this species is mainly due to the hard seed coat. Germination was not affected by light conditions, suggesting that seeds of this species are not photoblastic. Germination was higher at alternating day/night temperatures of 30/20 °C (74%) and 35/25 °C (69%) than at 25/15 °C (63%). Moderate salinity and water stress did not inhibit germination of H. tridactylites. Seedling emergence of H. tridactylites was highest (57%) for the seeds buried at a 2 cm depth in the soil; 18% of seedlings emerged from seeds buried at 8 cm but no seedlings emerged below this depth. Soil inversion by tillage to bury weed seeds below their maximum depth of emergence could serve an important tool for managing H. tridactylites.

  19. A bio-indicator for the evaluation of quality forestry and landscape fragmentation

    Directory of Open Access Journals (Sweden)

    Kappers EF

    2013-11-01

    Full Text Available A bio-indicator for the evaluation of quality forestry and landscape fragmentation. Intensive agricultural practices, as well as tourism development, summer fires, urbanization and air pollution represent a serious threat for many woodlands in Mediterranean Europe. Tawny owls, Strix aluco, is a valuable indicator of habitat quality and shows high sensitivity to wood fragmentation. Assessing the association between Tawny owls and their habitat may provide useful tools for conservation and management of forested habitats. Populations of woodland birds are influenced by forest characteristics, wood proportion being a key factor explaining breeding density and regularity in nest spacing. Populations of the Tawny Owl reach their highest densities in old deciduous forests. The distribution of territories remains almost constant for many years, and the period during which any particular wood maintains suitable conditions for nesting depends on factors like tree species and management, especially on the timing and extent of thinning. To assure the maintenance of good habitat quality in most woodlands, regulation of water diversion, prevention of summer fires, and a general reduction of human activities inside forests seem to be useful conservation tools.

  20. Bioindicator and fish health studies around the Terra Nova oil development site on the Grand Banks

    Energy Technology Data Exchange (ETDEWEB)

    Mathieu, A.; Hanlon, J.; Melvin, W.; French, B. [Oceans Ltd., St. John' s, NL (Canada); DeBlois, E. [Elisabeth DeBlois Inc., St. John' s, NL (Canada); Williams, U.; Wight, F.; Janes, G. [Petro-Canada, St. John' s, NL (Canada)

    2009-07-01

    Bioindicators or health effect indicators can be a valuable reconnaissance tool for addressing concerns on the part of the fishing industry and public interests regarding any potential impacts of pollutants on fish stocks. American plaice (Hippoglossoides platessoides) was initially chosen by the oil industry as an indicator species for Environmental Effects Monitoring (EEM) programs in the Grand Banks of Newfoundland because it is an important commercial flatfish. This presentation reported on fish health studies carried out at the Terra Nova development site before and after release of produced waters, which began in 2003. These studies represented 1 component of the overall Terra Nova EEM program. Fish were collected near the development area and in a reference area 20 km away. A broad range of health effect indicators were studied, including fish condition, visible skin and organ lesions, levels of mixed-function oxygenase (MFO) enzymes, haematology and an array of 19 histopathological indices in liver and gills. These indicators have been widely used in laboratory and field investigations with various fish species. A slight elevation of MFO enzyme activity was observed in fish from the development area in 2002, before release of produced water. In 2006, other indices were similar between the development and reference area. It was concluded that the overall results do not indicate any project effects.

  1. Growth tree rings as bioindicator of environmental pollution employing SR-TXRF

    International Nuclear Information System (INIS)

    Faria, Bruna Fernanda; Moreira, Silvana; Medeiros, Jean Gabriel da Silva; Tomazello Filho, Mario; Zucchi, Orgheda Luiza Araujo Domingues

    2007-01-01

    The present work has the aim to evaluate the use of tree ring as a bioindicator of the environmental pollution in urban and industrial areas. The sampling was made in a area located at the Forest Park, in the Bauru city, Sao Paulo state/Brazil. The area was contaminated by battery industries residues, which was interdicted by the Environmental Control Agency of the Sao Paulo State (CETESB), on March, 2002. Trees of Copaifera langsdorffi L. species were sampling in two different sites. The first one is near to the industry and other site 1700 m far. Synchrotron Radiation Total Reflection X-Ray Fluorescence technique (SR-TXRF) was employed to identify and quantify the metals of toxicological importance in the wood samples. The analysis was performed in the Brazilian Synchrotron Light Laboratory using a white beam for excitation and a hyperpure germanium detector for X-ray detection. In wood samples Cr, Co, Ni, Cu, Zn, As and Pb were determined. (author)

  2. Growth tree rings as bioindicator of environmental pollution employing SR-TXRF

    Energy Technology Data Exchange (ETDEWEB)

    Faria, Bruna Fernanda; Moreira, Silvana [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Faculdade de Engenharia, Arquitetura e Urbanismo]. E-mail: silvana@fec.unicamp.br; Vives, Ana Elisa Sirito de [Universidade Metodista de Piracicaba (UNIMEP), Santa Barbara D' Oeste, SP (Brazil)]. E-mail: aesvives@unimep.br; Medeiros, Jean Gabriel da Silva; Tomazello Filho, Mario [Universidade de Sao Paulo (USP), SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz]. E-mail: jeangm@esalq.usp.br; Zucchi, Orgheda Luiza Araujo Domingues [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas]. E-mail: olzucchi@fcfrp.usp.br

    2007-07-01

    The present work has the aim to evaluate the use of tree ring as a bioindicator of the environmental pollution in urban and industrial areas. The sampling was made in a area located at the Forest Park, in the Bauru city, Sao Paulo state/Brazil. The area was contaminated by battery industries residues, which was interdicted by the Environmental Control Agency of the Sao Paulo State (CETESB), on March, 2002. Trees of Copaifera langsdorffi L. species were sampling in two different sites. The first one is near to the industry and other site 1700 m far. Synchrotron Radiation Total Reflection X-Ray Fluorescence technique (SR-TXRF) was employed to identify and quantify the metals of toxicological importance in the wood samples. The analysis was performed in the Brazilian Synchrotron Light Laboratory using a white beam for excitation and a hyperpure germanium detector for X-ray detection. In wood samples Cr, Co, Ni, Cu, Zn, As and Pb were determined. (author)

  3. Using lichens as bioindicators of air pollution deposition near remote mining operations

    International Nuclear Information System (INIS)

    Berryman, S.; Straker, J.; Straker, D.

    2009-01-01

    The feasibility of using biological receptors to characterize pollutant deposition across a large study area was examined. The unique characteristics of lichens render them suitable for use in air pollution assessment and monitoring. These non-vascular plants absorb nutrients directly from the atmosphere, while also accumulating atmospheric contaminants. The elemental content in lichens collected near remote industrial sources can help determine spatial patterns of atmospheric pollutant deposition, including fugitive dust escapement from mining and milling operations and atmospheric emissions from processing and mine fleet operations. This paper presented 2 studies in which lichens were used as bioindicators of air pollution. The first study involved the use of epiphytic lichens in the Athabasca oil sands region of northeastern Alberta. Lichen elemental content revealed patterns of nitrogen and sulphur deposition in the region. An analysis for trace metals was used to identify specific pollutant sources. The second study involved the use of terrestrial lichen to evaluate off-site airborne transport of metals from the Anvil Range open-pit lead-zinc mine in south-central Yukon. The study revealed elevated lead and zinc concentrations in lichens up to 20 km from the mine site. The studies confirmed the utility of lichens as biomonitors of pollutant deposition. It was concluded that several objectives regarding the mitigation of environmental impacts from mining operations can be addressed using this approach. 23 refs., 1 tab., 1 fig.

  4. Placental cadmium as an additional noninvasive bioindicator of active maternal tobacco smoking.

    Science.gov (United States)

    Piasek, Martina; Jurasović, Jasna; Sekovanić, Ankica; Brajenović, Nataša; Brčić Karačonji, Irena; Mikolić, Anja; Grgec, Antonija Sulimanec; Stasenko, Sandra

    2016-01-01

    Tobacco smoke (TS) is a mixture of chemicals that is known to exert carcinogenic and endocrine-disrupting effects, as well as adverse effects on various systems. In TS nicotine is the major alkaloid and cadmium (Cd) the most abundant metal ion. The aim of this investigation was to assess exposure to Cd attributed to TS in healthy postpartum subjects (mean age 28 years) after term vaginal delivery in a clinical hospital by determining metal levels in maternal blood, placenta, and cord blood in relation to nicotine in maternal hair (12-cm-long samples). Two study groups were compared based upon self-reporting data: smokers (n = 32; continual cigarette smoking 3 months before and 9 months during pregnancy) and nonsmokers (n = 54; including passive smokers whose parameters did not differ from unexposed nonsmokers). In smokers compared to nonsmokers maternal hair nicotine concentrations increased approximately sevenfold, while Cd levels rose fourfold in maternal blood and up to twofold in placenta. Significant positive correlations were noted between maternal hair nicotine and placental Cd, maternal hair nicotine and maternal blood Cd, and placental Cd and maternal blood Cd. Levels of cord blood Cd were low in both study groups (bioindicator in addition to commonly used noninvasive hair nicotine in maternal TS assessment, especially in cases where unavailable or inappropriate (short or chemically treated) hair samples occur.

  5. The Combined Toxic and Genotoxic Effects of Cd and As to Plant Bioindicator Trifolium repens L

    Science.gov (United States)

    Ghiani, Alessandra; Fumagalli, Pietro; Nguyen Van, Tho; Gentili, Rodolfo; Citterio, Sandra

    2014-01-01

    This study was undertaken to investigate combined toxic and genotoxic effects of cadmium (Cd) and arsenic (As) on white clover, a pollutant sensitive plant frequently used as environmental bioindicator. Plants were exposed to soil spiked with increasing concentrations of cadmium sulfate (20, 40 and 60 mg Kg−1) or sodium arsenite (5, 10 and 20 mg Kg−1) as well as with their combinations. Metal(loid) bioavailability was assessed after soil contamination, whereas plant growth, metal(loid) concentration in plant organs and DNA damage were measured at the end of plant exposition. Results showed that individual and joint toxicity and genotoxicity were related to the concentration of Cd and As measured in plant organs, and that As concentration was the most relevant variable. Joint effects on plant growth were additive or synergistic, whereas joint genotoxic effects were additive or antagonistic. The interaction between Cd and As occurred at both soil and plant level. In soil the presence of As limited the bioavailability of Cd, whereas the presence of Cd increased the bioavailability of As. Nevertheless only As biovailability determined the amount of As absorbed by plants. The amount of Cd absorbed by plant was not linearly correlated with the fraction of bioavailable Cd in soil suggesting the involvement of additional factors, such as plant uptake mechanisms. These results reveal that the simultaneous presence in soil of Cd and As, although producing an additive or synergistic toxic effect on Trifolium repens L. growth, generates a lower DNA damage. PMID:24914541

  6. Use of bioindicators and passive sampling devices to evaluate ambient ozone concentrations in north central Pennsylvania

    International Nuclear Information System (INIS)

    Yuska, D.E.; Skelly, J.M.; Ferdinand, J.A.; Stevenson, R.E.; Savage, J.E.; Mulik, J.D.; Hines, A.

    2003-01-01

    Passive samplers and bioindicator plants detect ozone air pollution in north central Pennsylvania. - Ambient concentrations of tropospheric ozone and ozone-induced injury to black cherry (Prunus serotina) and common milkweed (Asclepias syriaca) were determined in north central Pennsylvania from 29 May to 5 September 2000 and from 28 May to 18 September 2001. Ogawa passive ozone samplers were utilized within openings at 15 forested sites of which six were co-located with TECO model 49 continuous ozone monitors. A significant positive correlation was observed between the Ogawa passive samplers and the TECO model 49 continuous ozone monitors for the 2000 (r=0.959) and 2001 (r=0.979) seasons. In addition, a significant positive correlation existed in 2000 and 2001 between ozone concentration and elevation (r=0.720) and (r=0.802), respectively. Classic ozone-induced symptoms were observed on black cherry and common milkweed. In 2000, initial injury was observed in early June, whereas for the 2001 season, initial injury was initially observed in late June. During both seasons, injury was noted at most sites by mid- to late-July. Soil moisture potential was measured for the 2001 season and a significant positive relationship (P<0.001) showed that injury to black cherry was a function of cumulative ozone concentrations and available soil moisture

  7. Use of bioindicators and passive sampling devices to evaluate ambient ozone concentrations in north central Pennsylvania

    Energy Technology Data Exchange (ETDEWEB)

    Yuska, D.E.; Skelly, J.M.; Ferdinand, J.A.; Stevenson, R.E.; Savage, J.E.; Mulik, J.D.; Hines, A

    2003-09-01

    Passive samplers and bioindicator plants detect ozone air pollution in north central Pennsylvania. - Ambient concentrations of tropospheric ozone and ozone-induced injury to black cherry (Prunus serotina) and common milkweed (Asclepias syriaca) were determined in north central Pennsylvania from 29 May to 5 September 2000 and from 28 May to 18 September 2001. Ogawa passive ozone samplers were utilized within openings at 15 forested sites of which six were co-located with TECO model 49 continuous ozone monitors. A significant positive correlation was observed between the Ogawa passive samplers and the TECO model 49 continuous ozone monitors for the 2000 (r=0.959) and 2001 (r=0.979) seasons. In addition, a significant positive correlation existed in 2000 and 2001 between ozone concentration and elevation (r=0.720) and (r=0.802), respectively. Classic ozone-induced symptoms were observed on black cherry and common milkweed. In 2000, initial injury was observed in early June, whereas for the 2001 season, initial injury was initially observed in late June. During both seasons, injury was noted at most sites by mid- to late-July. Soil moisture potential was measured for the 2001 season and a significant positive relationship (P<0.001) showed that injury to black cherry was a function of cumulative ozone concentrations and available soil moisture.

  8. Spectral Bio-indicator Simulations for Tracking Photosynthetic Activities in a Corn Field

    Science.gov (United States)

    Cheng, Yen-Ben; Middleton, Elizabeth M.; Huemmrich, K. Fred; Zhang, Qingyuan; Corp, Lawrence; Campbell, Petya; Kustas, William

    2011-01-01

    Accurate assessment of vegetation canopy optical properties plays a critical role in monitoring natural and managed ecosystems under environmental changes. In this context, radiative transfer (RT) models simulating vegetation canopy reflectance have been demonstrated to be a powerful tool for understanding and estimating spectral bio-indicators. In this study, two narrow band spectroradiometers were utilized to acquire observations over corn canopies for two summers. These in situ spectral data were then used to validate a two-layer Markov chain-based canopy reflectance model for simulating the Photochemical Reflectance Index (PRI), which has been widely used in recent vegetation photosynthetic light use efficiency (LUE) studies. The in situ PRI derived from narrow band hyperspectral reflectance exhibited clear responses to: 1) viewing geometry which affects the asset of light environment; and 2) seasonal variation corresponding to the growth stage. The RT model (ACRM) successfully simulated the responses to the variable viewing geometry. The best simulations were obtained when the model was set to run in the two layer mode using the sunlit leaves as the upper layer and shaded leaves as the lower layer. Simulated PRI values yielded much better correlations to in situ observations when the cornfield was dominated by green foliage during the early growth, vegetative and reproductive stages (r = 0.78 to 0.86) than in the later senescent stage (r = 0.65). Further sensitivity analyses were conducted to show the important influences of leaf area index (LAI) and the sunlit/shaded ratio on PRI observations.

  9. Microalga Euglena as a bioindicator for testing genotoxic potentials of organic pollutants in Taihu Lake, China.

    Science.gov (United States)

    Li, Mei; Gao, Xiangyu; Wu, Bing; Qian, Xin; Giesy, John P; Cui, Yibin

    2014-05-01

    The microalga Euglena was selected as a bioindicator for determining genotoxicity potencies of organic pollutants in Meiliang Bay of Taihu Lake, Jiangsu, China among seasons in 2008. Several methods, including the comet assay to determine breaks in DNA and quantification of antioxidant enzymes were applied to characterize genotoxic effects of organic extracts of water from Taihu Lake on the flagellated, microalga Euglena gracilis. Contents of photosynthetic pigments, including Chl a, Chl b and carotenoid pigments were inversely proportion to concentrations of organic extracts to which E. gracilis was exposed. Organic extracts of Taihu Lake water also affected activities of superoxide dismutase (SOD) and peroxidase (POD) of E. gracilis. There were no statistically significant differences in SOD activities among seasons except in June but significant differences in POD activities were observed among all seasons. The metrics of DNA fragmentation in the alkaline unwinding assay (Comet assay), olive tail moment (OTM) and tail moment (TM), used as measurement endpoints during the genotoxicity assay were both greater when E. gracilis was exposed to organic of water collected from Taihu Lake among four seasons. It is indicated that the comet assay was useful for determining effects of constituents of organic extracts of water on E. gracilis and this assay was effective as an early warning to organic pollutants.

  10. Blood-borne biomarkers and bioindicators for linking exposure to health effects in environmental health science.

    Science.gov (United States)

    Wallace, M Ariel Geer; Kormos, Tzipporah M; Pleil, Joachim D

    2016-01-01

    Environmental health science aims to link environmental pollution sources to adverse health outcomes to develop effective exposure intervention strategies that reduce long-term disease risks. Over the past few decades, the public health community recognized that health risk is driven by interaction between the human genome and external environment. Now that the human genetic code has been sequenced, establishing this "G × E" (gene-environment) interaction requires a similar effort to decode the human exposome, which is the accumulation of an individual's environmental exposures and metabolic responses throughout the person's lifetime. The exposome is composed of endogenous and exogenous chemicals, many of which are measurable as biomarkers in blood, breath, and urine. Exposure to pollutants is assessed by analyzing biofluids for the pollutant itself or its metabolic products. New methods are being developed to use a subset of biomarkers, termed bioindicators, to demonstrate biological changes indicative of future adverse health effects. Typically, environmental biomarkers are assessed using noninvasive (excreted) media, such as breath and urine. Blood is often avoided for biomonitoring due to practical reasons such as medical personnel, infectious waste, or clinical setting, despite the fact that blood represents the central compartment that interacts with every living cell and is the most relevant biofluid for certain applications and analyses. The aims of this study were to (1) review the current use of blood samples in environmental health research, (2) briefly contrast blood with other biological media, and (3) propose additional applications for blood analysis in human exposure research.

  11. Melanomacrophage centers aggregation in P. lineatus spleen as bioindicator of environmental change

    Directory of Open Access Journals (Sweden)

    Sadiyappan Balamurugan

    2012-10-01

    Full Text Available Objective: To monitor the water quality parameters and climatic impact on the health condition of the fish through pathology study of Marine catfish spleen using Melano-macrophage centers (MMCs aggregation as a biomarker Marine catfishes along the parangipettai coast of Tamil Nadu. Methods: Replicate samples were done for all the seasons along the coast. Condition factor (K and Histopathology technique, were used to evaluate the condition. Results: The result revealed the Condition factor (K of fish was minimum in winter 1.0874 and maximum in summer 1.6726. Histology show the number of MMCs aggregation in spleen 59.5 暲8.3 MMCs/mm2 was observed in at post monsoon and 2-5 MMCs/mm2 in summer. Anova shows significantly different at P<0.05. The MMCs aggregation excited 30/mm2 when the water temperature is below 22曟and reaches maximum at temperatures of below 20 曟. The MMCs were correlated with water temperature shows positive correlation mean drop in water temperature may also reason for MMCs aggregation in spleen. Conclusion: The rise in temperature causes effect in health of the fish which was indicated in condition factor and MMCs aggregation. Animals are vulnerable to disease due to lowering of temperature and other environmental conditions. The MMCs aggregation can be used as bio-indicators for environmental.

  12. Palatal Rugae Patterns as a Bioindicator for Forensic Identification in Kodava and Tibetan Populations of India.

    Science.gov (United States)

    Shetty, Deeksha Kiran; Mali, Sheetal; Divakar, Hegde Deepak; Amit, Patil; Dhairaysheel, Edake; Harsh, Unadkat

    2015-01-01

    Palatal rugae are distinct to each individual and are highly specific to each individual as their fingerprints. Rugae pattern are specific to different racial groups making it convenient for population identification. Palatal rugae retain their shape throughout life and hence can be useful as an identification tool. The present study aimed at analyzing the differences in rugae pattern among Kodavas and Tibetan populations of Coorg, India and to examine if there was a difference in the rugae pattern between males and females within each group. The study comprised of 30 participants between 18 and 30 years of age, equally distributed between genders from each group. Examination of maxillary cast after tracing the rugae patterns was carried out. Rugae pattern was classified as "straight," "wavy," "curved," "circular" and "unification." Statistical analysis was done using SPSS 16 software. Non-parametric Mann-Whitney test was used for pairwise comparison of two populations. Mann-Whitney two-tailed test was used to test the difference between males and females. Results showed that wavy pattern was highest for both the groups. Circular rugae were totally absent in both the groups. There was a significant difference between Kodavas (mean = 1.000) and Tibetans (mean = 0.13) for unification pattern (P = 0.001). Palatal rugae patterns can be used as a bioindicator for human identification.

  13. Should apple snail Pomacea canaliculata (Caenogastropoda, Ampullariidae) be used as bioindicator for BDE-209?

    Science.gov (United States)

    Koch, Eduardo; Altamirano, Jorgelina Cecilia; Covaci, Adrian; Lana, Nerina Belén; Ciocco, Néstor Fernando

    2014-01-01

    Apple snail Pomacea canaliculata has been reported to accumulate polybrominated diphenyl ethers (PBDEs) and was recently proposed as PBDE bioindicator. This work investigates the ability of P. canaliculata to accumulate BDE-209 by dietary exposure under controlled experimental conditions. A 30-day long enrichment feeding assay was carried out using 30 adult apple snails, placed in individual aquaria. Food was enriched at three BDE-209 concentrations (400, 4,700, and 8,300 μg g(−1) lipid weight). Correlation between BDE-209 values in food and snail tissue were estimated according to Stockholm Convention suggested criteria for chemicals with K(OW) >5. All animals survived with no evident physical alterations, and all of them accumulated BDE-209. BDE-209 levels in tissue samples increased exponentially with the exposure concentration. The bioaccumulation factor vs. food concentration plot showed a peculiar pattern, in which at intermediate concentrations the snails accumulated less BDE-209 than expected. Our results suggest that P. canaliculata would present a detoxification mechanism for BDE-209 different from the most commonly reported metabolic pathways.

  14. Assessment of both environmental cytotoxicity and trace metal pollution using Populus simonii Carr. as a bioindicator.

    Science.gov (United States)

    Sluchyk, Victor; Sluchyk, Iryna; Shyichuk, Alexander

    2014-10-01

    The level of environmental pollution in the city of Ivano-Frankivsk (Western Ukraine) has been assessed by means of roadside poplar trees as bioindicators. Dividable apical meristem cells of rudimentary leaves were quantitatively analysed for mitotic activity and distribution. Anaphases were further examined for chromosomal aberrations. Male catkins were also examined for sterile pollens. Accumulation of trace elements in vegetative buds was also evaluated in order to reveal source(s) of environmental pollution. Poplar trees growing in the urban environment proved to have increased chromosomal aberrations (up to 4-fold) and increased pollen sterility (up to 4-fold) as well as decreased mitotic activity (by factor 1.5) as compared to control sampling site. The biomarker data correlate moderately with increased (up to 4-fold) concentrations of Ni, Zn, Pb, Cd and Cu in vegetative tissues suggesting that probable cause of the environmental cytotoxicity may be vehicle emissions. The maximum increase in chromosomal aberrations (7-fold) and the minimum mitotic activity (half of the control one) were recorded in poplar trees growing in industrial suburb in vicinity of large cement production plant. Taking in mind insignificant bioaccumulation of trace elements in the industrial suburb, the high environmental toxicity has been ascribed to contamination in cement and asbestos particulates.

  15. Marine environment status assessment based on macrophytobenthic plants as bio-indicators of heavy metals pollution.

    Science.gov (United States)

    Zalewska, Tamara; Danowska, Beata

    2017-05-15

    The main aim of study was to develop the environmental quality standards (EQS MP ) for selected heavy metals: Pb, Cd, Hg and Ni bioaccumulated in the tissues of marine macrophytobenthic plants: Chara baltica, Cladophora spp., Coccotylus truncatus, Furcellaria lumbricalis, Polysiphonia fucoides, Stuckenia pectinata and Zanichellia palustris, collected in designated areas of the southern Baltic Sea in period 2008-2015. The calculated concentration ratios (CR), which attained very high values: 10 4 Lkg -1 for lead, 10 3 Lkg -1 for nickel and mercury and even 10 5 Lkg -1 for cadmium formed the basis for the determination of EQS MP values. The EQS MP values were: 26mgkg -1 d.w. for Pb, 33mgkg -1 d.w. for Cd, 32mgkg -1 d.w. for Ni and 0.4mgkg -1 d.w. for Hg. The application of macrophytobenthic plants as bioindicators in marine environment status assessment of certain areas of the Baltic Sea is also described in the paper. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Influences of air pollution on the growth of ornamental tree species-particularly with reference to SO/sub 2/

    Energy Technology Data Exchange (ETDEWEB)

    Kim, T.W.

    1975-01-01

    For the purpose of detecting resistance to air pollution, particularly SO/sub 2/ contamination, six ornamental tree species were selected, i.e., Ginkgo biloba, Larix leptolepis, Pinus rigida, Syringa dilatata, Hibiscus syriacus, and Forsythia koreana. The sensitivity was observed and analyzed on the basis of the area ratio of smoke injury spot to the total leaf area. According to the results, the decreasing order of SO/sub 2/ sensitivity by species could be arranged as follows: (1) Hibiscus syriacus, (2) Ginkgo biloba, (3) Forsythia koreana, (4) Syringa dilatata, (5) Larix leptolepis, and (6) Pinus rigida. In general, Hibiscus syriacus and Ginkgo biloba can be grouped as the most resistant ones and Larix leptolepis and Pinus rigida as the weakest ones and Forsythia koreana and Syringa dilatata as the intermediate. Due to the sprouting ability and the formative ability of adventitious buds, the recovery from the SO/sub 2/ fumigation was prominent in Hibiscus syriacus, Syringa dilatata and Forsythia koreana. The differences in the smoke spot color were recognized by species, namely, dirt brown in Syringa dilatata, brilliant yellow brown in Pinus rigida and Ginkgo biloba, whitish yellow in Hibiscus syriacus, and red brown in Forsythia koreana. In the case of Ginkgo biloba and Larix leptolepis, the younger leaves were more resistant to SO/sub 2/ than the old ones. The sulfur content of leaves showed that on the basis of %/dry weight, broad-leaved species contained the higher amount of sulfur than the coniferous species. 15 ornamental tree species which have been growing in Seoul city were sampled from the 19 air polluted spots. The elucidated were the heavily polluted regions and the lightly polluted regions. The SO/sub 2/ absorbing capacities by species are explained in the text. 17 references.

  17. Bioclimatic thresholds, thermal constants and survival of mealybug, Phenacoccus solenopsis (hemiptera: pseudococcidae) in response to constant temperatures on hibiscus.

    Science.gov (United States)

    Sreedevi, Gudapati; Prasad, Yenumula Gerard; Prabhakar, Mathyam; Rao, Gubbala Ramachandra; Vennila, Sengottaiyan; Venkateswarlu, Bandi

    2013-01-01

    Temperature-driven development and survival rates of the mealybug, Phenacoccussolenopsis Tinsley (Hemiptera: Pseudococcidae) were examined at nine constant temperatures (15, 20, 25, 27, 30, 32, 35 and 40°C) on hibiscus (Hibiscusrosa -sinensis L.). Crawlers successfully completed development to adult stage between 15 and 35°C, although their survival was affected at low temperatures. Two linear and four nonlinear models were fitted to describe developmental rates of P. solenopsis as a function of temperature, and for estimating thermal constants and bioclimatic thresholds (lower, optimum and upper temperature thresholds for development: Tmin, Topt and Tmax, respectively). Estimated thresholds between the two linear models were statistically similar. Ikemoto and Takai's linear model permitted testing the equivalence of lower developmental thresholds for life stages of P. solenopsis reared on two hosts, hibiscus and cotton. Thermal constants required for completion of cumulative development of female and male nymphs and for the whole generation were significantly lower on hibiscus (222.2, 237.0, 308.6 degree-days, respectively) compared to cotton. Three nonlinear models performed better in describing the developmental rate for immature instars and cumulative life stages of female and male and for generation based on goodness-of-fit criteria. The simplified β type distribution function estimated Topt values closer to the observed maximum rates. Thermodynamic SSI model indicated no significant differences in the intrinsic optimum temperature estimates for different geographical populations of P. solenopsis. The estimated bioclimatic thresholds and the observed survival rates of P. solenopsis indicate the species to be high-temperature adaptive, and explained the field abundance of P. solenopsis on its host plants.

  18. Synthesis of silver nanoparticles by radiolysis, photolysis and chemical reduction of AgNO3 in Hibiscus sabdariffa infusion (karkade)

    International Nuclear Information System (INIS)

    Cataldo, Franco; Ursini, Ornella; Angelini, Giancarlo

    2016-01-01

    Silver nanoparticles of different average diameters were synthesized by γ-radiolysis, UV-photolysis and chemical reduction of AgNO 3 solutions in Hibiscus sabdariffa infusion commonly known as 'karkade'. The UV-photolysis was performed either by using a conventional Hg low pressure lamp emitting at 254 nm and also by using a new compact UV-LED source emitting at 360 nm. The kinetics rate constant of silver nanoparticles synthesis produced by γ-radiolysis and UV photolysis were determined and the average diameter of the resulting nanoparticles was estimated. (author)

  19. PHYSIOLOGICAL RESPONSES OF HIBISCUS ROSA-SINENSIS L. (MALVACEAE) AT “EL AGUSTINO” HILL, LIMA, PERU

    OpenAIRE

    La Rosa, Rafael; Acuña, Rosalyn; Acurio, Karina; Castillo, Adelina; Cepeda, Carla; Chavarry, Christian; Correa, Marco; De la Cruz, Luis; García, Mary; Huamani, Miluska; Jauregui, Julio; Luyo, Luz; Villanueva, Fiorella

    2011-01-01

    Physiological responses of Hibiscus rosa-sinensis L. (Malvaceae) were evaluated during April – June 2010. Four plants cultivated in introduced agricultural soil located on El Agustino hill, El Agustino district, Lima, Peru were randomly selected. During the period evaluated air temperature dropped from 29.4ºC to 17.2ºC and relative humidity increased from 40.50 to79.75%. Leaf area temperature showed only a slight decrease of 23.8 ºC to 16.5 ºC compared to the air temperature. T...

  20. Preliminary Study on Kinetic Solid-Liquid Extraction and Bio-Active Components Analysis of Hibiscus rosa-sinensis Leaves

    International Nuclear Information System (INIS)

    Saiful Irwan Zubairi; Haizulizam Suradi; Syazwan Aizad Abdul Mutalib; Zetty Shafiqa Othman; Norshahida Bustaman; Wan Ros Maryana Wan Musa

    2014-01-01

    Hibiscus rosa-sinensis or commonly known as 'Hibiscus' is a kind of decorative flowers which often grown in a subtropical countries. This plant is often used in the preparation of traditional medicine because of its pharmacological properties that are capable of treating number of health problems. The plant contains several essential bio-active substances and nutrients especially in its flowers and leaves. One of the bioactive substances is β-sitosterol which is abundant in the leaf crude extract. Hibiscus leaves water extract yielded mucilage which is widely used in lowering high body temperature due to fever (antipyretic). Therefore, the main objective of this paper was to determine the maximum concentration of mucilage and time of exhaustive extraction from fresh and dried leaves using a Peleq's mathematical model. Moreover, several analyses were conducted such as qualitative analysis to determine the presence of bioactive substances using high performance liquid chromatography (HPLC) and determination of functional groups by means of fourier transform infrared method (FTIR). Physical properties of the extracts were evaluated to determine its acidity and viscosity of the mucilage with respect to different shear stress. The result show that the extract of dried Hibiscus leaves exhibited high in its concentrations even though the achieved exhaustive extraction was relatively slow as compared to fresh leaves (p < 0.05). Based on the HPLC analysis, the main bio-active substances of β-sitosterol was only existed in fresh leaves samples. The absence of β-sitosterol in dried leaves extract was caused by the loss of other important bio-active substances which possess surfactant capability, due to thermal degradation of drying process or the component itself was deteriorated during the pre-preparation drying process. Furthermore, the FTIR analysis shows that the same composition of the absorption peaks for both extracts with the highest absorption of O-H bonds was