WorldWideScience

Sample records for biocompatible materials

  1. Cycloolefin copolymer: new biocompatible material

    Czech Academy of Sciences Publication Activity Database

    Starý, Zdeněk; Kruliš, Zdeněk; Řihošková, V.; Petrtýl, M.

    Bratislava : Ústav polymérov, 2004. s. 126. [Slovensko-české dni o polyméroch /3./. 26.09.2004-29.09.2004, Smolenice] R&D Projects: GA ČR GA106/03/0255 Keywords : ultra high molecular weight polyethylene ( UHMWPE ) Subject RIV: JI - Composite Materials

  2. [Biocompatibility of dental materials: Part 2. Materials with mucosal contact].

    Science.gov (United States)

    Klötzer, W T; Reuling, N

    1990-08-01

    Dental materials which are supposed to contact the oral, mucous membranes during their intended dental use may affect our patients health in different ways. Their local and systemic toxicity, and their allergenic and tumorigenic potential are reviewed and methods of biocompatibility testing discussed. Special emphasis is placed on impression materials, denture base resins and dental alloys. PMID:2269166

  3. Material Biocompatibility for PCR Microfluidic Chips

    KAUST Repository

    Kodzius, Rimantas

    2010-04-23

    As part of the current miniaturization trend, biological reactions and processes are being adapted to microfluidics devices. PCR is the primary method employed in DNA amplification, its miniaturization is central to efforts to develop portable devices for diagnostics and testing purposes. A problem is the PCR-inhibitory effect due to interaction between PCR reagents and the surrounding environment, which effect is increased in high-surface-are-to-volume ration microfluidics. In this study, we evaluated the biocompatibility of various common materials employed in the fabrication of microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most of the cases, addition of bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, whereas they did show noticeable interaction with the DNA polymerase. Our test, instead of using microfluidic devices, can be easily conducted in common PCR tubes using a standard bench thermocycler. Our data supports an overview of the means by which the materials most bio-friendly to microfluidics can be selected.

  4. Fabrication of Biocompatible, Vibrational Magnetoelastic Materials for Controlling Cellular Adhesion

    OpenAIRE

    Rajachar, Rupak M.; Keat Ghee Ong; Ee Lim Tan; Hal R. Holmes

    2012-01-01

    This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of ...

  5. Biocompatibility of root-end filling materials: recent update.

    Science.gov (United States)

    Saxena, Payal; Gupta, Saurabh Kumar; Newaskar, Vilas

    2013-08-01

    The purpose of a root-end filling is to establish a seal between the root canal space and the periradicular tissues. As root-end filling materials come into contact with periradicular tissues, knowledge of the tissue response is crucial. Almost every available dental restorative material has been suggested as the root-end material of choice at a certain point in the past. This literature review on root-end filling materials will evaluate and comparatively analyse the biocompatibility and tissue response to these products, with primary focus on newly introduced materials. PMID:24010077

  6. BIOCOMPATIBLE FLUORESCENT MICROSPHERES: SAFE PARTICLES FOR MATERIAL PENETRATION STUDIES

    Energy Technology Data Exchange (ETDEWEB)

    Farquar, G; Leif, R

    2009-07-15

    Biocompatible polymers with hydrolyzable chemical bonds have been used to produce safe, non-toxic fluorescent microspheres for material penetration studies. The selection of polymeric materials depends on both biocompatibility and processability, with tailored fluorescent properties depending on specific applications. Microspheres are composed of USFDA-approved biodegradable polymers and non-toxic fluorophores and are therefore suitable for tests where human exposure is possible. Micropheres were produced which contain unique fluorophores to enable discrimination from background aerosol particles. Characteristics that affect dispersion and adhesion can be modified depending on use. Several different microsphere preparation methods are possible, including the use of a vibrating orifice aerosol generator (VOAG), a Sono-Tek atomizer, an emulsion technique, and inkjet printhead. Applications for the fluorescent microspheres include challenges for biodefense system testing, calibrants for biofluorescence sensors, and particles for air dispersion model validation studies.

  7. Biodegradation and biocompatibility of mechanically active magnetoelastic materials

    International Nuclear Information System (INIS)

    Magnetoelastic (ME) materials have many advantages for use as sensors and actuators due to their wireless, passive nature. This paper describes the application of ME materials as biodegradable implants with controllable degradation rates. Experiments have been conducted to show that degradation rates of ME materials are dependent on the material compositions. In addition, it was shown that the degradation rates of the ME materials can be controlled remotely by applying a magnetic field, which causes the ME materials to generate low-magnitude vibrations that hasten their degradation rates. Another concern of ME materials for medical applications is biocompatibility. Indirect cytotoxicity analyses were performed on two types of ME materials: Metglas™ 2826 MB (FeNiMoB) and iron–gallium alloy. While results indicate Metglas is not biocompatible, the degradation products of iron–gallium materials have shown no adverse effects on cell viability. Overall, these results present the possibility of using ME materials as biodegradable, magnetically-controlled active implants. (paper)

  8. Cytotoxicity and biocompatibility of direct and indirect pulp capping materials

    Directory of Open Access Journals (Sweden)

    Karin Cristina da Silva Modena

    2009-12-01

    Full Text Available There are several studies about the cytotoxic effects of dental materials in contact with the pulp tissue, such as calcium hydroxide (CH, adhesive systems, resin composite and glass ionomer cements. The aim of this review article was to summarize and discuss the cytotoxicity and biocompatibility of materials used for protection of the dentin-pulp complex, some components of resin composites and adhesive systems when placed in direct or indirect contact with the pulp tissue. A large number of dental materials present cytotoxic effects when applied close or directly to the pulp, and the only material that seems to stimulate early pulp repair and dentin hard tissue barrier formation is CH.

  9. Fabrication of Biocompatible, Vibrational Magnetoelastic Materials for Controlling Cellular Adhesion

    Directory of Open Access Journals (Sweden)

    Rupak M. Rajachar

    2012-02-01

    Full Text Available This paper describes the functionalization of magnetoelastic (ME materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.

  10. Biocompatible Polymeric Materials Intended for Drug Delivery and Therapeutic Applications

    DEFF Research Database (Denmark)

    Hvilsted, Søren; Javakhishvili, Irakli; Bednarek, Melania;

    2007-01-01

    elucidating strategies for combination of biocompatible polymers in unique but amphiphilic manners. The targeted, documented biocompatible polymers like polycapro-lactone (PCL), poly(2-methoxyethyl acrylate)2 (PMEA) with the highest known blood compatibility, poly(methyl methacrylate) (PMMA), and the two...

  11. Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models

    OpenAIRE

    Bratlie, Kaitlin M.; Dang, Tram T.; Lyle, Stephen; Nahrendorf, Matthias; Weissleder, Ralph; Langer, Robert; Anderson, Daniel G.

    2010-01-01

    Background: Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis. Methodology/Principal Findings: Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Material...

  12. Effect of microwave power on EPR spectra of natural and synthetic dental biocompatible materials

    Directory of Open Access Journals (Sweden)

    Adamczyk Jakub

    2015-07-01

    Full Text Available Paramagnetic centers in the two exemplary synthetic and natural dental biocompatible materials applied in implantology were examined by the use of an X-band (9.3 GHz electron paramagnetic resonance (EPR spectroscopy. The EPR spectra were measured in the range of microwave power 2.2–70 mW. The aims of this work were to compare paramagnetic centers concentrations in different dental biocompatible materials and to determine the effect of microwave power on parameters of their EPR spectra. It is the very first and innovatory examination of paramagnetic centers in these materials. It was pointed out that paramagnetic centers existed in both natural (~1018 spin/g and synthetic (~1019 spin/g dental biocompatible materials, but the lower free radical concentration characterized the natural sample. Continuous microwave saturation of EPR spectra indicated that faster spin-lattice relaxation processes existed in synthetic dental biocompatible materials than in natural material. Linewidths (ΔBpp of the EPR spectra of the natural dental material slightly increased for the higher microwave powers. Such effect was not observed for the synthetic material. The broad EPR lines (ΔBpp: 2.4 mT, 3.9 mT, were measured for the natural and synthetic dental materials, respectively. Probably strong dipolar interactions between paramagnetic centers in the studied samples may be responsible for their line broadening. EPR spectroscopy is the useful experimental method in the examination of paramagnetic centers in dental biocompatible materials.

  13. Biocompatibility of coronary stent materials: effect of electrochemical polishing

    Energy Technology Data Exchange (ETDEWEB)

    Scheerder, I. de [University Hospital Leuven (Belgium). Dept. of Cardiology; Sohier, J.; Froyen, L.; Humbeeck, J. van [Louvain Univ. (Belgium). Dept. of Metallurgy and Materials Engineering; Verbeken, E. [University Hospital Leuven (Belgium). Dept. of Pathology

    2001-02-01

    Percutaneous Transluminal Coronary Revascularization (PTCR) is now a widely accepted treatment modality for atherosclerotic coronary artery disease. Current multicenter randomized trials comparing PTCR with the more invasive Coronary Artery Bypass Grafting could not show long-term significant survival differences. During the last two decades progress has been made to further optimize PTCR. The most logic approach to treat atherosclerotic coronary narrowings is to remove the atherosclerotic material using especially developed devices. Several trials, however, could not show a significant beneficial outcome after use of these devices compared to plain old balloon angioplasty. Another approach was to implant a coronary prothesis (stent), scaffolding the diseased coronary artery after PTCA. This approach resulted in a decreased restenosis rate at follow-up. The beneficial effects of stenting, however, was not found to be related to the inhibition of the neointimal cellular proliferation after vascular injury, but simply to be the mechanical result of overstretching of the treated vessel segment. The most important remaining clinical problem after stenting remains the neointimal hyperplasia within the stent, resulting in a significant stent narrowing in 13 to 30% of patients. Further efforts to improve the clinical results of coronary stenting should focus on the reduction of this neointimal hyperplasia. Neointimal hyperplasia after stent implantation results from (1) a healting response to the injury caused by the stent implantation and (2) a foreign body response to the stent itself. Factors that seem to influence the neointimal hyperplastic response are genetic, local disease related, stent delivery related and stent related factors. Biocompatibilisation of coronary stents by looking for more biocompatible metal alloys, optimized surface characteristics and optimized stent designs should result in a better late patency. Furthermore drug eluting and radioactive stents

  14. Novel biocompatible materials for in vivo two-photon polymerisation

    International Nuclear Information System (INIS)

    Two-photon polymerisation (2PP) is a versatile laser fabrication technique that allows the creation of 3D structures at micro- and nanometre precision. The structures are created additively in direct accordance to a computer-aided design (CAD). It requires tightly focused fs-pulsed light sources usually operating in the near infrared wavelength range. In this region, biological tissues exhibit a window of transparency and only absorb light minimally. When operating below a certain pulse energy threshold, the laser light does not cause any cellular damage. This theoretically allows inducing 2PP in the presence of living biological tissues and cells. Suitable biocompatible formulations that can render bioactive constructs would potentially allow building a dynamic environment with topographical, chemical and mechanical cues similar to that of the natural extracellular matrix. In that way, 2PP would allow to alter key elements of this environment without changing any other influencing factors. To explore these possibilities, 2PP has to overcome two main limitations, the slow process speeds and the lack of available optimised formulations. In this thesis, we report the design and realisation of a 2PP experimental setup, which allows fabricating hydrogel structures from novel water-based formulations. Writing speeds of above 100 mm/s are feasible, which is the highest speed reported in 2PP. Moreover, the presented components have the potential to be formed in vivo, in the presence of living cells and tissues. Using water-soluble two-photon optimised photoinitiators, we could effectively cross-link acrylates in formulations of up to 80% water content. As acrylates show a tendency towards Michael addition to proteins, we explored the use of vinyl ester and vinyl carbonate monomers for 2PP. In contrast to acrylic polymers, which form potentially toxic poly (acrylic acid), vinyl ester and carbonate polymers form biocompatible poly (vinyl alcohol) during degradation

  15. The Use of Nanoscaled Fibers or Tubes to Improve Biocompatibility and Bioactivity of Biomedical Materials

    Directory of Open Access Journals (Sweden)

    Xiaoming Li

    2013-01-01

    Full Text Available Nanofibers and nanotubes have recently gained substantial interest for potential applications in tissue engineering due to their large ratio of surface area to volume and unique microstructure. It has been well proved that the mechanical property of matrix could be largely enhanced by the addition of nanoscaled fibers or tubes. At present, more and more researches have shown that the biocompatibility and bioactivity of biomedical materials could be improved by the addition of nanofibers or nanotubes. In this review, the efforts using nanofibers and nanotubes to improve biocompatibility and bioactivity of biomedical materials, including polymeric nanofibers/nanotubes, metallic nanofibers/nanotubes, and inorganic nanofibers/nanotubes, as well as their researches related, are demonstrated in sequence. Furthermore, the possible mechanism of improving biocompatibility and bioactivity of biomedical materials by nanofibers or nanotubes has been speculated to be that the specific protein absorption on the nanoscaled fibers or tubes plays important roles.

  16. Effect of microwave power on EPR spectra of natural and synthetic dental biocompatible materials

    OpenAIRE

    Adamczyk Jakub; Ramos Paweł; Pilawa Barbara

    2015-01-01

    Paramagnetic centers in the two exemplary synthetic and natural dental biocompatible materials applied in implantology were examined by the use of an X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy. The EPR spectra were measured in the range of microwave power 2.2–70 mW. The aims of this work were to compare paramagnetic centers concentrations in different dental biocompatible materials and to determine the effect of microwave power on parameters of their EPR spectra. It i...

  17. Tissue reaction to sealing materials: different view at biocompatibility

    Directory of Open Access Journals (Sweden)

    Ghanaati S

    2010-11-01

    Full Text Available Abstract The biodegradability of root canal sealers in areas other than the root canal system is crucial to the overall success rate of endodontic treatment. The aim of the present study was to investigate, the cell and tissue reaction to GuttaFlow and AHPlus, both in vitro and in vivo. For the in vitro experiments the materials were incubated with Human Periodontal Ligament Fibroblasts and cell proliferation and cytotoxicity analyses were performed. Additional fluorescence-microscope stainings were carried out in order to visualize cell growth and morphology. For assessment of the tissue reaction to the materials a subcutaneous implantation model in Wistar rats was employed and the inflammatory response to the materials was visualized by means of general and specific histology after 6 weeks. Human gingival fibroblasts proliferation seemed to be dependent upon dental material and cultivation time. After an incubation period of 96 hrs AHPlus proved to be significantly (p

  18. The Biocompatibility and Biostability of New Cardiovascular Materials and Devices

    Science.gov (United States)

    Stokes, Ken

    Evaluating a new material for use in an implantable device is a complicated business. ISO 14971 is designed to assist in determining device-risk assessment. ISO 10993 is intended to help steer one through the evaluation of materials for implantable devices. An FDA Guidance is available specifically for pacemaker leads, but may be helpful for other devices as well. However, completing a battery of in vitro and in vivo tests does not necessarily qualify a material for implant, because the in vivo environment cannot be duplicated in vitro. In vivo materials testing helps, but is still insufficient because the device may have its own issues. Device implants in animals may get one to human clinical studies and market release. Even after this stage, appropriate postmarket surveillance is necessary to know for sure how the device is really performing.

  19. Novel Biocompatible Material Based on Solid-State Modified Chitosan for Laser Stereolithography

    OpenAIRE

    P.S. Timashev; K.N. Bardakova; Т.S. Demina; G.I. Pudovkina; М.М. Novikov; М.А. Markov; D.S. Asyutin; L.F. Pimenova; Е.А. Svidchenko; А.М. Ermakov; I. I. Selezneva; V.К. Popov; N.А. Konovalov; Т.А. Akopova; А.B. Solovieva

    2015-01-01

    The aim of the investigation was to develop a novel biodegradable material based on chitosan synthesized by solid-state technoogy, and to create based on biocompatible three-dimensional cell-carrying scaffolds using laser stereolithography. Materials and Methods. Reactive systems were developed based on chitosan grafted with allyl, polyethylene glycol diacrylate, and the photoinitiator Irgacure 2959. The structures were obtained using laser stereolithography setting LS-120 (Institute on L...

  20. Biocompatibility of surgical implants

    Science.gov (United States)

    Kaelble, D. H.

    1979-01-01

    Method of selecting biocompatible materials for surgical implants uses fracture mechanic relationships and surface energies of candidate materials in presence of blood plasma. Technique has been used to characterize 190 materials by parameters that reflect their biocompatibility.

  1. The Use of Nanoscaled Fibers or Tubes to Improve Biocompatibility and Bioactivity of Biomedical Materials

    OpenAIRE

    Fuzhai Cui; Qingling Feng; Yubo Fan; Bo Yu(Brookhaven National Lab); Lianwen Sun; Wei Liu; Rongrong Cui; Xiaoming Li; Fumio Watari

    2013-01-01

    Nanofibers and nanotubes have recently gained substantial interest for potential applications in tissue engineering due to their large ratio of surface area to volume and unique microstructure. It has been well proved that the mechanical property of matrix could be largely enhanced by the addition of nanoscaled fibers or tubes. At present, more and more researches have shown that the biocompatibility and bioactivity of biomedical materials could be improved by the addition of nanofibers or na...

  2. Biocompatible medical implant materials with binding sites for a biodegradable drug-delivery system

    OpenAIRE

    Al-Dubai H; Pittner G; Pittner F; Gabor F

    2011-01-01

    Haifa Al-Dubai1, Gisela Pittner1, Fritz Pittner1, Franz Gabor21Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria; 2Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, Vienna, AustriaAbstract: Feasibility studies have been carried out for development of a biocompatible coating of medical implant materials allowing the binding of biodegradable drug-delivery systems in a way that their reloading ...

  3. Novel Biocompatible Material Based on Solid-State Modified Chitosan for Laser Stereolithography

    Directory of Open Access Journals (Sweden)

    P.S. Timashev

    2015-09-01

    Full Text Available The aim of the investigation was to develop a novel biodegradable material based on chitosan synthesized by solid-state technoogy, and to create based on biocompatible three-dimensional cell-carrying scaffolds using laser stereolithography. Materials and Methods. Reactive systems were developed based on chitosan grafted with allyl, polyethylene glycol diacrylate, and the photoinitiator Irgacure 2959. The structures were obtained using laser stereolithography setting LS-120 (Institute on Laser and Information Technologies, Russian Academy of Sciences, Russia. Results. Partial replacement of chitosan amino groups by allyl groups (CТ-А and the introduction of polyethylene glycol diacrylate (PEG-DA as a crosslinking agent were found not to reduce the material biocompatibility. The metabolic activity determination of NCTC L929 cells using MTT assay showed that the samples under study to contain none water-soluble components toxic to mammalian cells. The samples based on CT-A and CT-A with a crosslinking agent PEG-DA are biocompatible and are able to support adhesion, spreading and proliferative activity of human mesenchymal stromal cells, but have significant differences in the extent and nature of the expression activation of gene markers for osteogenic differentiation path.

  4. Research on the preparation, biocompatibility and bioactivity of magnesium matrix hydroxyapatite composite material.

    Science.gov (United States)

    Linsheng, Li; Guoxiang, Lin; Lihui, Li

    2016-08-12

    In this paper, magnesium matrix hydroxyapatite composite material was prepared by electrophoretic deposition method. The optimal process parameters of electrophoretic deposition were HA suspension concentration of 0.02 kg/L, aging time of 10 days and voltage of 60 V. Animal experiment and SBF immersion experiment were used to test the biocompatibility and bioactivity of this material respectively. The SD rats were divided into control group and implant group. The implant surrounding tissue was taken to do tissue biopsy, HE dyed and organizational analysis after a certain amount of time in the SD rat body. The biological composite material was soaked in SBF solution under homeothermic condition. After 40 days, the bioactivity of the biological composite material was evaluated by testing the growth ability of apatite on composite material. The experiment results showed that magnesium matrix hydroxyapatite biological composite material was successfully prepared by electrophoretic deposition method. Tissue hyperplasia, connective tissue and new blood vessels appeared in the implant surrounding soft tissue. No infiltration of inflammatory cells of lymphocytes and megakaryocytes around the implant was found. After soaked in SBF solution, a layer bone-like apatite was found on the surface of magnesium matrix hydroxyapatite biological composite material. The magnesium matrix hydroxyapatite biological composite material could promot calcium deposition and induce bone-like apatite formation with no cytotoxicity and good biocompatibility and bioactivity. PMID:27567779

  5. [Electrochemical properties of biocompatible material hardness modifications on titanium and steel under mechanical loads].

    Science.gov (United States)

    Braun, W; Walter, U; Holbein, R; Thull, R

    2005-04-01

    Friction corrosion may appear between different implant components or between implant and hard tissue. The sliding micro movements induce fretting wear corrosion and have been recently reported as a cause of joint prostheses failure. A surface coating is desirable, that retains the mechanical properties of the substrate, offers good biocompatibility and improves the fretting corrosion resistance. In this study it could be demonstrated that tantalum and niobium coatings fulfill the requirements. On titanium substrates the coating decreases the abrasion against PMMA, an orthopedic relevant material. Furthermore, in the case of medical steel substrates the biocompatibility and the corrosion properties are improved. The better abrasion-resistance is minimizing the release of allergological critical particles like nickel and chromium. PMID:15884706

  6. Biocompatibility and cytotoxicity study of nanophotonic rigid gas permeable contact lens material

    International Nuclear Information System (INIS)

    Since materials on nanoscale have different characteristics from materials on macro scale their biocompatibility should be precisely and specifically investigated. Fullerenes, the third carbon allotrope, are one of the most used nanomaterials. The least stable and the most common is fullerene C60. One of the main disadvantages of fullerene is its low solubility in water. In order to make it soluble, it must be functionalized with polar groups such as -OH and -COOH. From all the water soluble fullerenes the most important ones are those with -OH groups attached named fullerols. We have developed new materials for contact lenses by adding fullerene (C60) and fullerol (C60(OH)24) into PMMA. The aim of our investigation was to compare the influences of those materials on aqueous solutions similar to tear film. For the analysis of the solutions we used opto-magnetic imaging and IR spectroscopy. The acquired spectrums were commented and compared with the standard contact lens material, which was analyzed by the same methods. The ISO 10993 cytotoxicity test on extract of nanophotonic material with incorporated C60 was done as well. This research contributes to better understanding of the biocompatibility of new rigid gas permeable contact lens materials.

  7. Biocompatibilidade dos materiais em Ortodontia: mito ou realidade? Biocompatibility of orthodontic materials: myth or reality?

    Directory of Open Access Journals (Sweden)

    Luciane Macedo de Menezes

    2009-04-01

    Full Text Available O objetivo deste trabalho é apresentar uma revisão sobre os conceitos relacionados à biocompatibilidade dos materiais empregados em Ortodontia. Fatos relacionados às reações de hipersensibilidade aos diversos materiais ortodônticos são discutidos, sendo apresentadas as condutas recomendáveis nestas situações.The aim of this paper is to present a review on the biocompatibility of orthodontic materials. Hypersensitivity reactions to these materials are discussed and the recommended conduct in this kind of situation are presented.

  8. Biocompatible self-assembly of nano-materials for Bio-MEMS and insect reconnaissance.

    Energy Technology Data Exchange (ETDEWEB)

    Brozik, Susan Marie; Cesarano, Joseph, III; Brinker, C. Jeffrey; Dunphy, Darren Robert; Sinclair, Michael B.; Manginell, Monica; Ashley, Carlee E. (University of New Mexico, Albuquerque, NM); Timlin, Jerilyn Ann; Werner-Washburne, Margaret C. (University of New Mexico, Albuquerque, NM); Calvert, Paul Davidson (University of Massachusetts - Dartmouth, Dartmouth, MA); Hartenberger, Tamara N.; Flemming, Jeb Hunter; Baca, Helen Kennicott (University of New Mexico, Albuquerque, NM)

    2003-12-01

    This report summarizes the development of new biocompatible self-assembly procedures enabling the immobilization of genetically engineered cells in a compact, self-sustaining, remotely addressable sensor platform. We used evaporation induced self-assembly (EISA) to immobilize cells within periodic silica nanostructures, characterized by unimodal pore sizes and pore connectivity, that can be patterned using ink-jet printing or photo patterning. We constructed cell lines for the expression of fluorescent proteins and induced reporter protein expression in immobilized cells. We investigated the role of the abiotic/biotic interface during cell-mediated self-assembly of synthetic materials.

  9. Biocompatible medical implant materials with binding sites for a biodegradable drug-delivery system

    Directory of Open Access Journals (Sweden)

    Al-Dubai H

    2011-10-01

    Full Text Available Haifa Al-Dubai1, Gisela Pittner1, Fritz Pittner1, Franz Gabor21Max F Perutz Laboratories, Department of Biochemistry, University of Vienna, Vienna, Austria; 2Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, Vienna, AustriaAbstract: Feasibility studies have been carried out for development of a biocompatible coating of medical implant materials allowing the binding of biodegradable drug-delivery systems in a way that their reloading might be possible. These novel coatings, able to bind biodegradable nanoparticles, may serve in the long run as drug carriers to mediate local pharmacological activity. After biodegradation of the nanoparticles, the binding sites could be reloaded with fresh drug-delivering particles. As a suitable receptor system for the nanoparticles, antibodies are anchored. The design of the receptor is of great importance as any bio- or chemorecognitive interaction with other components circulating in the blood has to be avoided. Furthermore, the binding between receptor and the particles has to be strong enough to keep them tightly bound during their lifetime, but on the other hand allow reloading after final degradation of the particles. The nanoparticles suggested as a drug-delivery system for medical implants can be loaded with different pharmaceuticals such as antibiotics, growth factors, or immunosuppressives. This concept may enable the changing of medication, even after implantation of the medical device, if afforded by patients’ needs.Keywords: antibody immobilization, biocompatible coating, chitosan nanoparticles, drug targeting, medical device

  10. Preparation, tribological properties and biocompatibility of fluorinated graphene/ultrahigh molecular weight polyethylene composite materials

    Science.gov (United States)

    Xu, L.; Zheng, Y.; Yan, Z.; Zhang, W.; Shi, J.; Zhou, F.; Zhang, X.; Wang, J.; Zhang, J.; Liu, B.

    2016-05-01

    Fluorinated graphene (FG)/ultra-high molecular weight polyethylene (UHMWPE) composites were successfully prepared by ultrasonic dispersion and liquid thermoforming method. The mechanical and tribological properties of pure UHMWPE and FG/UHMWPE composites were investigated using micro-hardness tester and high-speed reciprocating friction tester. The results showed that: adding FG could not only increase the micro-hardness of the composites, but also decrease the wear volume of the composite significantly. The friction coefficients of the composites were also reduced with the increasing of FG content. In addition, the MC3T3-E1 cells adhered and grew well on the surface of the FG/UHMWPE composites as observed by SEM and fluorescence microscope, indicating the addition of FG did not affect the morphology and activity of the cells. The FG/UHMWPE composites exhibited excellent mechanical properties, tribological properties and biocompatibility, which could be used as the potential artificial joint replacement material.

  11. Entrapping quercetin in silica/polyethylene glycol hybrid materials: Chemical characterization and biocompatibility.

    Science.gov (United States)

    Catauro, Michelina; Bollino, Flavia; Nocera, Paola; Piccolella, Simona; Pacifico, Severina

    2016-11-01

    Sol-gel synthesis was exploited to entrap quercetin, a natural occurring antioxidant polyphenol, in silica-based hybrid materials, which differed in their polyethylene glycol (PEG) content (6, 12, 24 and 50wt%). The materials obtained, whose nano-composite nature was ascertained by Scanning Electron Microscopy (SEM), were chemically characterized by Fourier Transform InfraRed (FT-IR) and UV-Vis spectroscopies. The results prove that a reaction between the polymer and the drug occurred. Bioactivity tests showed their ability to induce hydroxyapatite nucleation on the sample surfaces. The direct contact method was applied to screen the cytotoxicity of the synthetized materials towards fibroblast NIH 3T3 cells, commonly used for in vitro biocompatibility studies, and three nervous system cell lines (neuroblastoma SH-SY5Y, glioma U251, and pheochromocytoma PC12 cell lines), adopted as models in oxidative stress related studies. Using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay NIH 3T3 proliferation was assessed and the morphology was not compromised by direct exposure to the materials. Analogously, PC-12, and U-251 cell lines were not affected by new materials. SH-SY5Y appeared to be the most sensitive cell line with cytotoxic effects of 20-35%. PMID:27524014

  12. Reducing the cytotoxicity of inhalable engineered nanoparticles via in situ passivation with biocompatible materials

    Energy Technology Data Exchange (ETDEWEB)

    Byeon, Jeong Hoon, E-mail: postjb@yu.ac.kr [School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749 (Korea, Republic of); Park, Jae Hong; Peters, Thomas M. [Department of Occupational and Environmental Health, University of Iowa, IA 52242 (United States); Roberts, Jeffrey T., E-mail: jtrob@purdue.edu [Department of Chemistry, Purdue University, IN 47907 (United States)

    2015-07-15

    Highlights: • The cytotoxicity of model welding particles was modulated through in situ passivation. • Model welding particles were incorporated with chitosan nanoparticles for passivation. • In vitro assay revealed that the passivated particles had a lower cytotoxicity. • Passivation with chitosan adhesive or graphite paste could also reduce cytotoxicity. • This method would be suitable for efficient reduction of inhalable toxic components. - Abstract: The cytotoxicity of model welding nanoparticles was modulated through in situ passivation with soluble biocompatible materials. A passivation process consisting of a spark discharge particle generator coupled to a collison atomizer as a co-flow or counter-flow configuration was used to incorporate the model nanoparticles with chitosan. The tested model welding nanoparticles are inhaled and that A549 cells are a human lung epithelial cell line. Measurements of in vitro cytotoxicity in A549 cells revealed that the passivated nanoparticles had a lower cytotoxicity (>65% in average cell viability, counter-flow) than the untreated model nanoparticles. Moreover, the co-flow incorporation between the nanoparticles and chitosan induced passivation of the nanoparticles, and the average cell viability increased by >80% compared to the model welding nanoparticles. As a more convenient way (additional chitosan generation and incorporation devices may not be required), other passivation strategies through a modification of the welding rod with chitosan adhesive and graphite paste did also enhance average cell viability (>58%). The approach outlined in this work is potentially generalizable as a new platform, using only biocompatible materials in situ, to treat nanoparticles before they are inhaled.

  13. Reducing the cytotoxicity of inhalable engineered nanoparticles via in situ passivation with biocompatible materials

    International Nuclear Information System (INIS)

    Highlights: • The cytotoxicity of model welding particles was modulated through in situ passivation. • Model welding particles were incorporated with chitosan nanoparticles for passivation. • In vitro assay revealed that the passivated particles had a lower cytotoxicity. • Passivation with chitosan adhesive or graphite paste could also reduce cytotoxicity. • This method would be suitable for efficient reduction of inhalable toxic components. - Abstract: The cytotoxicity of model welding nanoparticles was modulated through in situ passivation with soluble biocompatible materials. A passivation process consisting of a spark discharge particle generator coupled to a collison atomizer as a co-flow or counter-flow configuration was used to incorporate the model nanoparticles with chitosan. The tested model welding nanoparticles are inhaled and that A549 cells are a human lung epithelial cell line. Measurements of in vitro cytotoxicity in A549 cells revealed that the passivated nanoparticles had a lower cytotoxicity (>65% in average cell viability, counter-flow) than the untreated model nanoparticles. Moreover, the co-flow incorporation between the nanoparticles and chitosan induced passivation of the nanoparticles, and the average cell viability increased by >80% compared to the model welding nanoparticles. As a more convenient way (additional chitosan generation and incorporation devices may not be required), other passivation strategies through a modification of the welding rod with chitosan adhesive and graphite paste did also enhance average cell viability (>58%). The approach outlined in this work is potentially generalizable as a new platform, using only biocompatible materials in situ, to treat nanoparticles before they are inhaled

  14. Biocompatibility and characterisation of a candidate microelectrode material for biosensor applications

    International Nuclear Information System (INIS)

    Recent advances in microcircuit technology have enabled the fabrication of Multiple Microelectrode Arrays (MEAs) for investigating the characteristics of networks of neuronal cells either in vivo or in vitro. When producing a MEA materials used must be corrosion resistant, have low electrical impedance and the materials must be biocompatible. Existing MEA's have limited life spans, relatively high impedance values and limited uses. Thus creating a requirement for new MEA technology. TiN thin films have become increasingly useful in a wide variety of applications, due to their nature, which includes chemical stability, high hardness, excellent wear and electrical properties and also biocompatibility. The favourable electrical and biocompatibility characteristics of thin films of TiN make them a possible candidate for use in a MEA. TiN thin films can be deposited by a number of methods including evaporation, ion plating and sputtering. The method of deposition, along with process parameters used can have a marked effect on the characteristics of TiN films, including changes in preferred orientation, hardness and wear and also biocompatibility. TiN thin films were deposited onto glass substrates by pulsed DC reactive sputtering of a Ti target, with Argon and nitrogen gas mixtures and labelled Type I TiN films. Also industrial TIN films deposited by Arc Ion plating were carefully selected for comparison and labelled Type II TiN films. The microstructure, composition, surface chemistry, surface topography and roughness were studied using X-Ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Atomic Force Microscopy (AFM) and Profilometry. Type I TIN films showed a surface topography similar to Zone I and Type II TiN films showed a surface topography similar to Zone 2 of the Movchan and Dernchishin structure zone model for sputtered films. XPS showed that the surface composition of all TiN films was predominantly TiO2, TiN and TiNxOy. Significant differences in

  15. A review of the methods used to study biocompatibility of Portland cement-derived materials used in dentistry

    OpenAIRE

    Camilleri, Josette

    2006-01-01

    Advanced restorative dentistry may necessitate the need for surgical intervention to the infected root apex. Once access to the root end is achieved, the root apex is resected and filled with a dental restorative material. The materials currently in use are not satisfactory due to inadequate biocompatibility and failure to achieve desirable properties in an aqueous environment. With the introduction of a new material, essentially Portland cement used in the building industry, these desirable ...

  16. A biocompatible hybrid material with simultaneous calcium and strontium release capability for bone tissue repair.

    Science.gov (United States)

    Almeida, J Carlos; Wacha, András; Gomes, Pedro S; Alves, Luís C; Fernandes, M Helena Vaz; Salvado, Isabel M Miranda; Fernandes, M Helena R

    2016-05-01

    The increasing interest in the effect of strontium in bone tissue repair has promoted the development of bioactive materials with strontium release capability. According to literature, hybrid materials based on the system PDMS-SiO2 have been considered a plausible alternative as they present a mechanical behavior similar to the one of the human bone. The main purpose of this study was to obtain a biocompatible hybrid material with simultaneous calcium and strontium release capability. A hybrid material, in the system PDMS-SiO2-CaO-SrO, was prepared with the incorporation of 0.05mol of titanium per mol of SiO2. Calcium and strontium were added using the respective acetates as sources, following a sol-gel technique previously developed by the present authors. The obtained samples were characterized by FT-IR, solid-state NMR, and SAXS, and surface roughness was analyzed by 3D optical profilometry. In vitro studies were performed by immersion of the samples in Kokubo's SBF for different periods of time, in order to determine the bioactive potential of these hybrids. Surfaces of the immersed samples were observed by SEM, EDS and PIXE, showing the formation of calcium phosphate precipitates. Supernatants were analyzed by ICP, revealing the capability of the material to simultaneously fix phosphorus ions and to release calcium and strontium, in a concentration range within the values reported as suitable for the induction of the bone tissue repair. The material demonstrated to be cytocompatible when tested with MG63 osteoblastic cells, exhibiting an inductive effect on cell proliferation and alkaline phosphatase activity. PMID:26952443

  17. Biocompatibility and tissue interactions of a new filler material for medical use.

    Science.gov (United States)

    Zarini, Elena; Supino, Rosanna; Pratesi, Graziella; Laccabue, Diletta; Tortoreto, Monica; Scanziani, Eugenio; Ghisleni, Gabriele; Paltrinieri, Saverio; Tunesi, Gianfranco; Nava, Maurizio

    2004-09-15

    Filler materials for medical use present limits, such as the induction of chronic inflammation and fibrosis. In the search for synthetic materials with improved biocompatible properties, a new polyacrylamide hydrogel, Aquamid (Contura SA, Montreux, Switzerland), has been investigated in preclinical systems. In cell cultures (endothelial cells and fibroblast), no or only transient biological effects were associated with 10% Aquamid exposure. The Aquamid-host interactions were examined in mice (10 mice per group) implanted subcutaneously or in the mammary fat pad with a very large volume (1.5 ml) of the material. Blood analysis, performed after 15 and 94 days (five mice per time for each group) to detect acute or late manifestations of toxicity, did not reveal relevant abnormalities in either group of Aquamid-bearing mice compared with control mice, except for a transient thrombocytopenia and a mild leukocytosis. Histological analysis of the pellet showed the presence of a thin, poorly vascularized cyst wall in implants. Only mild mesenchymal reparative and inflammatory processes were observed, even at longer observation times (more than 400 days). No alterations in any organ were detected. Despite the large volume implanted (approximately 5 percent of mouse body weight), the Aquamid pellet maintained its original size and shape without spreading or sticking to surrounding tissues. In conclusion, the study indicated a good tolerability of the new biopolymer in preclinical systems. The clinical utility of this new compound, if confirmed by clinical randomized trials showing its atoxic properties, could be in the field of aesthetic plastic surgery as a filler material for body contouring and in reconstructive surgery and above all in cancer patients to restore surgical defects. PMID:15468401

  18. Ultrashort pulse laser processing of hard tissue, dental restoration materials, and biocompatibles

    Science.gov (United States)

    Yousif, A.; Strassl, M.; Beer, F.; Verhagen, L.; Wittschier, M.; Wintner, E.

    2007-07-01

    During the last few years, ultra-short laser pulses have proven their potential for application in medical tissue treatment in many ways. In hard tissue ablation, their aptitude for material ablation with negligible collateral damage provides many advantages. Especially teeth representing an anatomically and physiologically very special region with less blood circulation and lower healing rates than other tissues require most careful treatment. Hence, overheating of the pulp and induction of microcracks are some of the most problematic issues in dental preparation. Up till now it was shown by many authors that the application of picosecond or femtosecond pulses allows to perform ablation with very low damaging potential also fitting to the physiological requirements indicated. Beside the short interaction time with the irradiated matter, scanning of the ultra-short pulse trains turned out to be crucial for ablating cavities of the required quality. One main reason for this can be seen in the fact that during scanning the time period between two subsequent pulses incident on the same spot is so much extended that no heat accumulation effects occur and each pulse can be treated as a first one with respect to its local impact. Extension of this advantageous technique to biocompatible materials, i.e. in this case dental restoration materials and titanium plasma-sprayed implants, is just a matter of consequence. Recently published results on composites fit well with earlier data on dental hard tissue. In case of plaque which has to be removed from implants, it turns out that removal of at least the calcified version is harder than tissue removal. Therefore, besides ultra-short lasers, also Diode and Neodymium lasers, in cw and pulsed modes, have been studied with respect to plaque removal and sterilization. The temperature increase during laser exposure has been experimentally evaluated in parallel.

  19. Enhanced biocompatibility and antibacterial property of polyurethane materials modified with citric acid and chitosan.

    Science.gov (United States)

    Liu, Tian-Ming; Wu, Xing-Ze; Qiu, Yun-Ren

    2016-08-01

    Citric acid (CA) and chitosan (CS) were covalently immobilized on polyurethane (PU) materials to improve the biocompatibility and antibacterial property. The polyurethane pre-polymer with isocyanate group was synthesized by one pot method, and then grafted with citric acid, followed by blending with polyethersulfone (PES) to prepare the blend membrane by phase-inversion method so that chitosan can be grafted from the membrane via esterification and acylation reactions eventually. The native and modified membranes were characterized by attenuated total reflectance-Fourier transform infrared spectroscope, X-ray photoelectron spectroscopy, scanning electron microscopy, water contact angle measurement, and tensile strength test. Protein adsorption, platelet adhesion, hemolysis assay, activated partial thromboplastin time, prothrombin time, thrombin time, and adsorption of Ca(2+) were executed to evaluate the blood compatibility of the membranes decorated by CA and CS. Particularly, the antibacterial activities on the modified membranes were evaluated based on a vitro antibacterial test. It could be concluded that the modified membrane had good anticoagulant property and antibacterial property. PMID:27102367

  20. Biocompatible high performance hyperbranched epoxy/clay nanocomposite as an implantable material

    International Nuclear Information System (INIS)

    Polymeric biomaterials are in extensive use in the domain of tissue engineering and regenerative medicine. High performance hyperbranched epoxy is projected here as a potential biomaterial for tissue regeneration. Thermosetting hyperbranched epoxy nanocomposites were prepared with Homalomena aromatica rhizome oil-modified bentonite as well as organically modified montmorillonite clay. Fourier transformed infrared spectroscopy, x-ray diffraction and scanning and transmission electron microscopic techniques confirmed the strong interfacial interaction of clay layers with the epoxy matrix. The poly(amido amine)-cured thermosetting nanocomposites exhibited high mechanical properties like impact resistance (>100 cm), scratch hardness (>10 kg), tensile strength (48–58 MPa) and elongation at break (11.9–16.6%). Cytocompatibility of the thermosets was found to be excellent as evident by MTT and red blood cell hemolytic assays. The nanocomposites exhibited antimicrobial activity against Staphylococcus aureus (ATCC 11632), Escherichia coli (ATCC 10536), Mycobacterium smegmatis (ATCC14468) and Candida albicans (ATCC 10231) strains. In vivo biocompatibility of the best performing nanocomposite was ascertained by histopathological study of the brain, heart, liver and skin after subcutaneous implantation in Wistar rats. The material supported the proliferation of dermatocytes without induction of any sign of toxicity to the above organs. The adherence and proliferation of cells endorse the nanocomposite as a non-toxic biomaterial for tissue regeneration. (paper)

  1. Synthesis, toxicity, biocompatibility, and biomedical applications of graphene and graphene-related materials

    Directory of Open Access Journals (Sweden)

    Gurunathan S

    2016-05-01

    Full Text Available Sangiliyandi Gurunathan, Jin-Hoi Kim Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea Abstract: Graphene is a two-dimensional atomic crystal, and since its development it has been applied in many novel ways in both research and industry. Graphene possesses unique properties, and it has been used in many applications including sensors, batteries, fuel cells, supercapacitors, transistors, components of high-strength machinery, and display screens in mobile devices. In the past decade, the biomedical applications of graphene have attracted much interest. Graphene has been reported to have antibacterial, antiplatelet, and anticancer activities. Several salient features of graphene make it a potential candidate for biological and biomedical applications. The synthesis, toxicity, biocompatibility, and biomedical applications of graphene are fundamental issues that require thorough investigation in any kind of applications related to human welfare. Therefore, this review addresses the various methods available for the synthesis of graphene, with special reference to biological synthesis, and highlights the biological applications of graphene with a focus on cancer therapy, drug delivery, bio-imaging, and tissue engineering, together with a brief discussion of the challenges and future perspectives of graphene. We hope to provide a comprehensive review of the latest progress in research on graphene, from synthesis to applications. Keywords: biomedical applications, cancer therapy, drug delivery, graphene, graphene-related materials, tissue engineering, toxicity 

  2. Fine structure analysis of biocompatible ceramic materials based hydroxyapatite and metallic biomaterials 316L

    International Nuclear Information System (INIS)

    The aim of this paper was to obtain and characterize (surface morphology and fine structure) two types of materials: Ca10(PO4)6(OH)2 hydroxyapatite powder (HAp) as biocompatible ceramic materials and AISI 316L austenitic stainless steels as metallic biomaterials, which are the components of the metal–ceramic composites used for medical implants in reconstructive surgery and prosthetic treatment. The HAp was synthesized by coprecipitation method, heat treated at 200 °C, 800 °C and 1200 °C for 4 h, analyzed by X-ray diffraction (XRD) and scanning electron microscope (SEM). The stainless steel 316L type was made by casting, annealing and machined with a low speed (100 mm/s) in order to obtain a smooth surface and after that has been studied from residual stresses point of view in three polishing regimes conditions: at low speed polishing (150 rpm), at high speed polishing (1500 rpm) and high speed-vibration contact polishing (1500 rpm) using wide angle X-ray diffractions (WAXD). The chemical compositions of AISI 316 steel samples were measured using a Foundry Master Spectrometer equipped with CCD detector for spectral lines and the sparking spots of AISI 316L samples were analyzed using SEM. By XRD the phases of HAp powders have been identified and also the degree of crystallinity and average size of crystallites, and with SEM, we studied the morphology of the HAp. It has been found from XRD analysis that we obtained HAp with a high degree of crystallinity at 800 °C and 1200 °C, no presence of impurity and from SEM analysis we noticed the influence of heat treatment on the ceramic particles morphology. From the study of residual stress profiles of 316L samples were observed that it differs substantially for different machining regimes and from the SEM analysis of sparking spots we revealed the rough surfaces of stainless steel rods necessary for a better adhesion of HAp on it.

  3. In vitro biocompatibility evaluation of ePTFE graft with controlled release of heparin from mesoporous material

    Energy Technology Data Exchange (ETDEWEB)

    Li Kun [Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008 (China); Zhou Yu; Yang Jia yuan; Zhu Jianhua [Key Laboratory of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China); Liu Changjian, E-mail: cjliu1331@gmail.com [Department of Vascular Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008 (China)

    2012-02-01

    Heparin-loaded mesoporous-expanded poly(tetrafluoroethylene) (ePTFE) vascular prosthesis (HMVP-n) are prepared and the biocompatibility is studied by contact angle, heparin release, platelet resistance, chromogenic assay, endothelial progenitor cells (EPCs) proliferation and produced-NO function, in order to illustrate the relationship between the performance of artificial vessels and their mesostructure. Through in situ synthesis of mesoporous silica on ePTFE grafts, different mesoporous silica materials can be uniformly coated on the surface as well as the internal fibers of the artificial vessels to give various mesoporous vascular prosthesis, named as MVP-n. Structure analysis through scanning electron microscopy (SEM), energy dispersive X-ray detector (EDX) analysis and nitrogen sorption experiment indicates that different MVP-n series own the similar n{sub F}/n{sub Si} ratio of both surface and cross-section, large Brunauer-Emmett-Tellerand (BET) surface area and average pore size located in meso range but different textural properties. Owning to the existence of mesostucture, controlled release and high bioactivity of heparin can be achieved, and the biocompatibility greatly enhance: surface hydrophilicity increases; no adherent platelet was observed on the surface of HMVP-n when they contacted with platelet-enriched plasma; endothelial progenitor cells proliferous potential and produced-NO function exhibit better endothelial coverage of grafts. And the performance is closely related to the mesostructure, suggesting a new way to improve the biocompatibility of biomaterials through controlling their mesostructure.

  4. In vitro biocompatibility evaluation of ePTFE graft with controlled release of heparin from mesoporous material

    International Nuclear Information System (INIS)

    Heparin-loaded mesoporous-expanded poly(tetrafluoroethylene) (ePTFE) vascular prosthesis (HMVP-n) are prepared and the biocompatibility is studied by contact angle, heparin release, platelet resistance, chromogenic assay, endothelial progenitor cells (EPCs) proliferation and produced-NO function, in order to illustrate the relationship between the performance of artificial vessels and their mesostructure. Through in situ synthesis of mesoporous silica on ePTFE grafts, different mesoporous silica materials can be uniformly coated on the surface as well as the internal fibers of the artificial vessels to give various mesoporous vascular prosthesis, named as MVP-n. Structure analysis through scanning electron microscopy (SEM), energy dispersive X-ray detector (EDX) analysis and nitrogen sorption experiment indicates that different MVP-n series own the similar nF/nSi ratio of both surface and cross-section, large Brunauer-Emmett-Tellerand (BET) surface area and average pore size located in meso range but different textural properties. Owning to the existence of mesostucture, controlled release and high bioactivity of heparin can be achieved, and the biocompatibility greatly enhance: surface hydrophilicity increases; no adherent platelet was observed on the surface of HMVP-n when they contacted with platelet-enriched plasma; endothelial progenitor cells proliferous potential and produced-NO function exhibit better endothelial coverage of grafts. And the performance is closely related to the mesostructure, suggesting a new way to improve the biocompatibility of biomaterials through controlling their mesostructure.

  5. In vitro biocompatibility evaluation of ePTFE graft with controlled release of heparin from mesoporous material

    Science.gov (United States)

    Li, Kun; Zhou, Yu; Yang, Jia yuan; Zhu, Jian hua; Liu, Chang jian

    2012-02-01

    Heparin-loaded mesoporous-expanded poly(tetrafluoroethylene) (ePTFE) vascular prosthesis (HMVP-n) are prepared and the biocompatibility is studied by contact angle, heparin release, platelet resistance, chromogenic assay, endothelial progenitor cells (EPCs) proliferation and produced-NO function, in order to illustrate the relationship between the performance of artificial vessels and their mesostructure. Through in situ synthesis of mesoporous silica on ePTFE grafts, different mesoporous silica materials can be uniformly coated on the surface as well as the internal fibers of the artificial vessels to give various mesoporous vascular prosthesis, named as MVP-n. Structure analysis through scanning electron microscopy (SEM), energy dispersive X-ray detector (EDX) analysis and nitrogen sorption experiment indicates that different MVP-n series own the similar nF/nSi ratio of both surface and cross-section, large Brunauer-Emmett-Tellerand (BET) surface area and average pore size located in meso range but different textural properties. Owning to the existence of mesostucture, controlled release and high bioactivity of heparin can be achieved, and the biocompatibility greatly enhance: surface hydrophilicity increases; no adherent platelet was observed on the surface of HMVP-n when they contacted with platelet-enriched plasma; endothelial progenitor cells proliferous potential and produced-NO function exhibit better endothelial coverage of grafts. And the performance is closely related to the mesostructure, suggesting a new way to improve the biocompatibility of biomaterials through controlling their mesostructure.

  6. Biocompatibility and characterisation of a candidate microelectrode material for biosensor applications

    CERN Document Server

    Cyster, L A

    2001-01-01

    TiN films. Results demonstrated how the deposition parameters could influence the crystallographic nature of TiN films. The 0.2 and 1.0 mu m Type I TiN and both Type II TiN films were selected to allow comparison oj the influence of surface chemistry versus topography on the biocompatibility of TiN films. Biocompatibility testing using 3T3-L1 fibroblasts and C6 Glial cells showed that at early time points TiN films with increased surface interstitial nitrogen content were more favourable for the attachment of increased numbers of cells. At later time points increased cell spreading was observed on the Type II TiN films indicating that increased grain size and topographical features resulted in increased cell spreading. Investigation of the biocompatibility of the 0.2 and 1.0 mu m Type I TiN films and both Type II TiN films with primary hippocampal neurones showed that surface chemistry with predominantly interstitial nitrogen resulted in neuronal network formation similar to the control substrate. Increased n...

  7. Material properties and in vitro biocompatibility of a newly developed bone cement

    Directory of Open Access Journals (Sweden)

    Elke Mitzner

    2009-01-01

    Full Text Available In this study mechanical properties and biocompatibility (In Vitro of a new bone cement were investigated. A new platform technology named COOL is a variable composite of dissolved, chemically modified PMMA and different bioceramics. COOL cures at body temperature via a classical cementation reaction. Compressive strengths ranging from 3.6 ± 0.8 to 62.8 ± 1.3 MPa and bending strengths ranging from 9.9 ± 2.4 to 26.4 ± 3.0 MPa were achieved with different COOL formulations. Porosity varied between 31 and 43%. Varying the components of each formulation mechanical properties and porosity could be adjusted. In Vitro biocompatibility studies with primary human osteoblasts (pHOB in direct contact with different COOL formulations, did not reveal any signs of toxicity. In contrast to Refobacin® R, cells incubated with COOL showed similar density, viability and ALP activity compared to control, if specimen were added immediately to the cell monolayer after preparation. In conclusion, COOL has promising mechanical properties in combination with high biocompatibility In Vitro and combines different advantages of both CPCs and PMMA cements by avoiding some of the respective shortcomings.

  8. Policaprolactone/polyvinylpyrrolidone/siloxane hybrid materials: Synthesis and in vitro delivery of diclofenac and biocompatibility with periodontal ligament fibroblasts.

    Science.gov (United States)

    Peña, José A; Gutiérrez, Sandra J; Villamil, Jean C; Agudelo, Natalia A; Pérez, León D

    2016-01-01

    In this paper, we report the synthesis of polycaprolactone (PCL) based hybrid materials containing hydrophilic domains composed of N-vinylpyrrolidone (VP), and γ-methacryloxypropyltrimethoxysilane (MPS). The hybrid materials were obtained by RAFT copolymerization of N-vinylpyrrolidone and MPS using a pre-formed dixanthate-end-functionalized PCL as macro-chain transfer agent, followed by a post-reaction crosslinking step. The composition of the samples was determined by elemental and thermogravimetric analyses. Differential scanning calorimetry and X-ray diffraction indicated that the crystallinity of PCL decreases in the presence of the hydrophilic domains. Scanning electron microscopy images revealed that the samples present an interconnected porous structure on the swelling. Compared to PCL, the hybrid materials presented low water contact angle values and higher elastic modulus. These materials showed controlled release of diclofenac, and biocompatibility with human periodontal ligament fibroblasts. PMID:26478287

  9. Material properties and in vitro biocompatibility of a newly developed bone cement

    OpenAIRE

    Elke Mitzner; Paco Albertus Hubert Maria Pelt; Christian Mueller; Angela Strohwig; Wolf-Dieter Mueller

    2009-01-01

    In this study mechanical properties and biocompatibility (In Vitro) of a new bone cement were investigated. A new platform technology named COOL is a variable composite of dissolved, chemically modified PMMA and different bioceramics. COOL cures at body temperature via a classical cementation reaction. Compressive strengths ranging from 3.6 ± 0.8 to 62.8 ± 1.3 MPa and bending strengths ranging from 9.9 ± 2.4 to 26.4 ± 3.0 MPa were achieved with different COOL formulations. Porosity varied bet...

  10. Carbon-Coated Gold Nanorods: A Facile Route to Biocompatible Materials for Photothermal Applications.

    Science.gov (United States)

    Kaneti, Yusuf Valentino; Chen, Chuyang; Liu, Minsu; Wang, Xiaochun; Yang, Jia Lin; Taylor, Robert Allen; Jiang, Xuchuan; Yu, Aibing

    2015-11-25

    Gold nanorods and their core-shell nanocomposites have been widely studied because of their well-defined anisotropy and unique optical properties and applications. This study demonstrates a facile hydrothermal synthesis strategy for generating carbon coating on gold nanorods (AuNRs@C) under mild conditions (glucose). The structure and composition of the produced core-shell nanocomposites were characterized using advanced microscopic and spectroscopic techniques. The functional properties, particularly the photothermal and biocompatibility properties of the produced AuNRs@C, were quantified to assess their potential in photothermal hyperthermia. These AuNRs@C were tested in vitro (under representative treatment conditions) using near-infrared (NIR) light irradiation. It was found that the AuNRs produced here exhibit exemplary heat generation capability. Temperature changes of 10.5, 9, and 8 °C for AuNRs@C were observed with carbon shell thicknesses of 10, 17, and 25 nm, respectively, at a concentration of 50 μM, after 600 s of irradiation with a laser power of 0.17 W/cm(2). In addition, the synthesized AuNRs@C also exhibit good biocompatibility toward two soft tissue sarcoma cell lines (HT1080, a fibrosarcoma; and GCT, a fibrous histiocytoma). The cell viability study shows that AuNRs@C (at a concentration of <0.1 mg/mL) core-shell particles induce significantly lower cytotoxicity on both HT1080 and GCT cell lines, as compared with cetyltrimethylammonium bromide (CTAB)-capped AuNRs. Furthermore, similar to PEG-modified AuNRs, they are also safe to both HT1080 and GCT cell lines. This biocompatibility results from a surface full of -OH or -COH groups, which are suitable for linking and are nontoxic Therefore, the AuNRs@C represent a viable alternative to PEG-coated AuNRs for facile synthesis and improved photothermal conversion. Overall, these findings open up a new class of carbon-coated nanostructures that are biocompatible and could potentially be employed in a

  11. Biocompatibility testing of novel starch-based materials with potential application in orthopaedic surgery: a preliminary study.

    Science.gov (United States)

    Mendes, S C; Reis, R L; Bovell, Y P; Cunha, A M; van Blitterswijk, C A; de Bruijn, J D

    2001-07-01

    This paper describes an extensive biocompatibility evaluation of biodegradable starch-based materials aimed at orthopaedic applications as temporary bone replacement/fixation implants. For that purpose, a polymer (starch/ethylene vinyl alcohol blend, SEVA-C) and a composite of SEVA-C reinforced with hydroxyapatite (HA) particles, were evaluated in both in vitro and in vivo assays. For the in vitro analysis cell culture methods were used. The in vivo tissue reactions were evaluated in an intramuscular and intracortical bone implantation model on goats, using light and scanning electron microscopy. A computerized image analysis system was used to obtain histomorphometric data regarding bone contact and remodelling after 6 and 12 weeks of implantation. In both in vitro and in vivo models, the SEVA-C-based materials did not induce adverse reactions, which in addition to their bone-matching mechanical properties makes them promising materials for bone replacement fixation. PMID:11426886

  12. Novel POSS-PCU Nanocomposite Material as a Biocompatible Coating for Quantum Dots.

    Science.gov (United States)

    Rizvi, Sarwat B; Yang, Shi Yu; Green, Mark; Keshtgar, Mo; Seifalian, Alexander M

    2015-12-16

    Quantum dots (QDs) are fluorescent nanoparticles with unique photophysical properties that enable them to potentially replace traditional organic dyes and fluorescent proteins in various bioimaging applications. However, the inherent toxicity of their cores based on cadmium salts limits their widespread biomedical use. We have developed a novel nanocomposite polymer emulsion based on polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) that can be used to coat quantum dots to nullify their toxicity and enhance photostability. Here we report the synthesis and characterization of a novel POSS-PCU nanocomposite polymer emulsion and describe its application for coating QDs for biological application. The polymer was synthesized by a process of emulsion polymerization and formed stable micelles of ∼33 nm in diameter. CdTe/CdS/ZnS QDs were efficiently stabilized by the polymer emulsion through encapsulation within the polymer micelles. Characterization studies showed no significant change in the unique photophysical properties of QDs after coating. The polymer was biocompatible to HepG2, HUVECs, and mouse skeletal muscle cells at 2.5% after 24 h exposure on in vitro testing. Polymer encapsulated QDs showed enhanced photostability on exposure to high degrees of UV irradiation and air as well as significantly reduced cytotoxicity on exposure to HepG2 cells at 30 μg/mL for 24 h. We have therefore concluded that the POSS-PCU polymer emulsion has the potential to make a biocompatible and photostable coating for QDs enabling a host of biomedical applications to take this technology to the next level. PMID:26439600

  13. Synthesis, toxicity, biocompatibility, and biomedical applications of graphene and graphene-related materials

    Science.gov (United States)

    Gurunathan, Sangiliyandi; Kim, Jin-Hoi

    2016-01-01

    Graphene is a two-dimensional atomic crystal, and since its development it has been applied in many novel ways in both research and industry. Graphene possesses unique properties, and it has been used in many applications including sensors, batteries, fuel cells, supercapacitors, transistors, components of high-strength machinery, and display screens in mobile devices. In the past decade, the biomedical applications of graphene have attracted much interest. Graphene has been reported to have antibacterial, antiplatelet, and anticancer activities. Several salient features of graphene make it a potential candidate for biological and biomedical applications. The synthesis, toxicity, biocompatibility, and biomedical applications of graphene are fundamental issues that require thorough investigation in any kind of applications related to human welfare. Therefore, this review addresses the various methods available for the synthesis of graphene, with special reference to biological synthesis, and highlights the biological applications of graphene with a focus on cancer therapy, drug delivery, bio-imaging, and tissue engineering, together with a brief discussion of the challenges and future perspectives of graphene. We hope to provide a comprehensive review of the latest progress in research on graphene, from synthesis to applications.

  14. Surface engineering of stainless steel materials by covalent collagen immobilization to improve implant biocompatibility.

    Science.gov (United States)

    Müller, Rainer; Abke, Jochen; Schnell, Edith; Macionczyk, Frank; Gbureck, Uwe; Mehrl, Robert; Ruszczak, Zbigniev; Kujat, Richard; Englert, Carsten; Nerlich, Michael; Angele, Peter

    2005-12-01

    It was shown recently that the deposition of thin films of tantalum and tantalum oxide enhanced the long-term biocompatibility of stainless steel biomaterials due to an increase in their corrosion resistance. In this study, we used this tantalum oxide coating as a basis for covalent immobilization of a collagen layer, which should result in a further improvement of implant tissue integration. Because of the high degradation rate of natural collagen in vivo, covalent immobilization as well as carbodiimide induced cross-linking of the protein was performed. It was found that the combination of the silane-coupling agent aminopropyl triethoxysilane and the linker molecule N,N'-disulphosuccinimidyl suberate was a very effective system for collagen immobilizing. Mechanical and enzymatic stability testing revealed a higher stability of covalent bound collagen layers compared to physically adsorbed collagen layers. The biological response induced by the surface modifications was evaluated by in vitro cell culture with human mesenchymal stem cells as well as by in vivo subcutaneous implantation into nude mice. The presence of collagen clearly improved the cytocompatibility of the stainless steel implants which, nevertheless, significantly depended on the cross-linking degree of the collagen layer. PMID:15967497

  15. Trehalose and Trehalose-based Polymers for Environmentally Benign, Biocompatible and Bioactive Materials

    Directory of Open Access Journals (Sweden)

    Mitsuhiro Shibata

    2008-08-01

    Full Text Available Abstract: Trehalose is a non-reducing disaccharide that is found in many organisms but not in mammals. This sugar plays important roles in cryptobiosis of selaginella mosses, tardigrades (water bears, and other animals which revive with water from a state of suspended animation induced by desiccation. The interesting properties of trehalose are due to its unique symmetrical low-energy structure, wherein two glucose units are bonded face-to-face by 1→1-glucoside links. The Hayashibara Co. Ltd., is credited for developing an inexpensive, environmentally benign and industrial-scale process for the enzymatic conversion of α-1,4-linked polyhexoses to α,α-D-trehalose, which made it easy to explore novel food, industrial, and medicinal uses for trehalose and its derivatives. Trehalosechemistry is a relatively new and emerging field, and polymers of trehalose derivatives appear environmentally benign, biocompatible, and biodegradable. The discriminating properties of trehalose are attributed to its structure, symmetry, solubility, kinetic and thermodynamic stability and versatility. While syntheses of trehalose-based polymer networks can be straightforward, syntheses and characterization of well defined linear polymers with tailored properties using trehalose-based monomers is challenging, and typically involves protection and deprotection of hydroxyl groups to attain desired structural, morphological, biological, and physical and chemical properties in the resulting products. In this review, we will overview known literature on trehalose’s fascinating involvement in cryptobiology; highlight its applications in many fields; and then discuss methods we used to prepare new trehalose-based monomers and polymers and explain their properties.

  16. Nanostructured Ti-Zr-Pd-Si-(Nb) bulk metallic composites: Novel biocompatible materials with superior mechanical strength and elastic recovery.

    Science.gov (United States)

    Hynowska, A; Blanquer, A; Pellicer, E; Fornell, J; Suriñach, S; Baró, M D; Gebert, A; Calin, M; Eckert, J; Nogués, C; Ibáñez, E; Barrios, L; Sort, J

    2015-11-01

    The microstructure, mechanical behaviour, and biocompatibility (cell culture, morphology, and cell adhesion) of nanostructured Ti45 Zr15 Pd35- x Si5 Nbx with x = 0, 5 (at. %) alloys, synthesized by arc melting and subsequent Cu mould suction casting, in the form of rods with 3 mm in diameter, are investigated. Both Ti-Zr-Pd-Si-(Nb) materials show a multi-phase (composite-like) microstructure. The main phase is cubic β-Ti phase (Im3m) but hexagonal α-Ti (P63/mmc), cubic TiPd (Pm3m), cubic PdZr (Fm3m), and hexagonal (Ti, Zr)5 Si3 (P63/mmc) phases are also present. Nanoindentation experiments show that the Ti45 Zr15 Pd30 Si5 Nb5 sample exhibits lower Young's modulus than Ti45 Zr15 Pd35 Si5 . Conversely, Ti45 Zr15 Pd35 Si5 is mechanically harder. Actually, both alloys exhibit larger values of hardness when compared with commercial Ti-40Nb, (HTi-Zr-Pd-Si ≈ 14 GPa, HTi-Zr-Pd-Si-Nb ≈ 10 GPa and HTi-40Nb ≈ 2.7 GPa). Concerning the biological behaviour, preliminary results of cell viability performed on several Ti-Zr-Pd-Si-(Nb) discs indicate that the number of live cells is superior to 94% in both cases. The studied Ti-Zr-Pd-Si-(Nb) bulk metallic system is thus interesting for biomedical applications because of the outstanding mechanical properties (relatively low Young's modulus combined with large hardness), together with the excellent biocompatibility. PMID:25533018

  17. Venom conjugated polylactide applied as biocompatible material for passive and active immunotherapy against scorpion envenomation.

    Science.gov (United States)

    Ayari-Riabi, Sana; Trimaille, Thomas; Mabrouk, Kamel; Bertin, Denis; Gigmes, Didier; Benlasfar, Zakaria; Zaghmi, Ahlem; Bouhaouala-Zahar, Balkiss; Elayeb, Mohamed

    2016-04-01

    Scorpion envenoming represents a public health issue in subtropical regions of the world. Treatment and prevention need to promote antitoxin immunity. Preserving antigenic presentation while removing toxin effect remains a major challenge in toxin vaccine development. Among particulate adjuvant, particles prepared with poly (d,l-lactide) polymer are the most extensively investigated due to their excellent biocompatibility and biodegradability. The aim of this study is to develop surfactant-free PLA nanoparticles that safely deliver venom toxic fraction to enhance specific immune response. PLA nanoparticles are coated with AahG50 (AahG50/PLA) and BotG50 (BotG50/PLA): a toxic fraction purified from Androctonus australis hector and Buthus occitanus tunetanus venoms, respectively. Residual toxicities are evaluated following injections of PLA-containing high doses of AahG50 (or BotG50). Immunization trials are performed with the detoxified fraction administered alone without adjuvant. A comparative study of the effect of Freund is also included. The neutralizing capacity of sera is determined in naive mice. Six months later, immunized mice are challenged subcutaneously with increased doses of AahG50. Subcutaneous lethal dose 50 (LD50) of AahG50 and BotG50 is of 575μg/kg and 1300μg/kg respectively. By comparison, BotG50/PLA is totally innocuous while 50% of tested mice survive 2875μg AahG50/kg. Alhydrogel and Freund are not able to detoxify such a high dose. Cross-antigenicity between particulate and soluble fraction is also, ensured. AahG50/PLA and BotG50/PLA induce high antibody levels in mice serum. The neutralizing capacity per mL of anti-venom was 258μg/mL and 186μg/mL calculated for anti-AahG50/PLA and anti-BotG50/PLA sera, respectively. Animals immunized with AahG50/PLA are protected against AahG50 injected dose of 3162μg/kg as opposed all non-immunized mice died at this dose. We find that the detoxification approach based PLA nanoparticles, benefit the

  18. Selection of Easily Accessible PCR- and Bio-Compatible Materials for Microfluidic Chips

    KAUST Repository

    Xiao, Kang

    2013-10-30

    Conventional fabrication of microfluidic chip is a complicated and time, effort and material consuming process. Consequently, due to high expenses, it has poor applicability for performing mass biological analysis by microfluidics. In this study, we repor

  19. Physical properties and biocompatibility of UHMWPE-derived materials modified by synchrotron radiation

    OpenAIRE

    Bykova, Iu.; Weinhardt, V.; Kashkarova, A.; Lebedev, S.; Baumbach, T.; Pichugin, V.; Zaitsev, K.; Khlusov, I.

    2014-01-01

    The applications of synchrotron radiation (SR) in medical imaging have become of great use, particularly in angiography, bronchography, mammography, computed tomography, and X-ray microscopy. Thanks to recently developed phase contrast imaging techniques non-destructive preclinical testing of low absorbing materials such as polymers has become possible. The focus of the present work is characterization and examination of UHMWPE-derived materials widely used in medicine, before and after their...

  20. Biocompatibility of Titanium

    Science.gov (United States)

    Namavar, Fereydoon; Sabirianov, Renat; Marton, Denes; Rubinstein, Alexander; Garvin, Kevin

    2012-02-01

    Titanium is the material of choice for orthopaedic applications because of its known biocompatibility. In order to enhance osteogenic properties of the Ti implants, it is necessary to understand the origin of its biocompatibility. We addresses the origin of Ti biocompatibility through (1) theoretical modeling, (2) the precise determination of Ti surface chemistry by X-ray photoelectron spectroscopy (XPS), (3) and the study of fibronectin adsorption as a function of Ti (near) surface chemistry by Enzyme-linked immunosorbent assay (ELISA). We compare the protein adsorption on Ti with the native oxide layer and the one coated by TiO2 in anatase phase using ion beam assisted deposition (IBAD). We show that the thin native sub-stoichiometric titanium oxide layer is crucial for biocompatibility of Ti surface. This is due to the enhancement of the non-specific adsorption of proteins which mediate cell adhesion. Improving the surface oxide quality, i.e. fabricating stoichiometric TiO2 (using IBAD) as well as nanoengineering the surface topology that matches its dimensions to that of adhesive proteins, is crucial for increased protein adsorption and, as a result, further increases biocompatibility of Ti implant materials.

  1. PCR biocompatibility of Lab-on-a-chip and MEMS materials

    DEFF Research Database (Denmark)

    Christensen, Troels Balmer; Pedersen, Christian Møller; Grøndahl, K. G.;

    2007-01-01

    DNA amplification using the polymerase chain reaction (PCR) is an important tool in biotechnology, pathogen surveillance in food, medical and forensic science etc. The PCR technique is now an important part of the research in and development of miniaturized biochemical analysis systems. However......, reduced or no DNA amplification at all is an important challenge for microfabricated PCR devices due to a negative interaction between PCR chemicals and the surrounding environment, i.e. the materials encapsulating the PCR mix. Materials of special interest regarding PCR compatibility are silicon, glass...... and polymers, which are important in the fabrication of microelectromechanical systems (MEMS), micro total analysis systems (mu TAS) and lab-on-a-chip (LOC) systems. The PCR inhibition effect is a particularly important phenomenon in microsystems due to an increased surface-to-volume ratio which...

  2. Structural and Electrochemical Properties of Functionalized Nanocellulose Materials and Their Biocompatibility

    OpenAIRE

    Carlsson, Daniel O.

    2014-01-01

    Nanocellulose has received considerable interest during the last decade because it is renewable and biodegradable, and has excellent mechanical properties, nanoscale dimensions and wide functionalization possibilities. It is considered to be a unique and versatile platform on which new functional materials can be based. This thesis focuses on nanocellulose from wood (NFC) and from Cladophora algae (CNC), functionalized with surface charges or coated with the conducting polymer polypyrrole (PP...

  3. Micromechanical properties of biocompatible materials for bone tissue engineering produced by direct 3D printing

    Czech Academy of Sciences Publication Activity Database

    Koudelka_ml., P.; Doktor, T.; Kytýř, Daniel; Fenclová, Nela; Šepitka, J.; Lukeš, J.

    Vol. 662. Durnten: Trans Tech Publications, 2015 - (Kovalčíková, A.; Lofaj, F.), s. 138-141 ISBN 978-3-03835-555-7. ISSN 1013-9826. [Conference on Local Mechanical Properties (LMP 2014) /11./. Stará Lesná (SK), 12.11.2014-14.11.2014] Institutional support: RVO:68378297 Keywords : bone scaffold * nanoindenation * mechanical properties * additive manufacturing Subject RIV: JJ - Other Materials http://www.scientific.net/KEM.662.138

  4. A novel method to control hydrolytic degradation of nanocomposite biocompatible materials via imparting superhydrophobicity

    Science.gov (United States)

    Khakbaz, Mobina; Hejazi, Iman; Seyfi, Javad; Jafari, Seyed-Hassan; Khonakdar, Hossein Ali; Davachi, Seyed Mohammad

    2015-12-01

    Acceleration of hydrolytic degradation of biomedical materials is not always desirable. For instance, terpolymers based on L-lactide, glycolide and trimethylene carbonate exhibit very fast hydrolytic degradation due to their amorphous structure, hydrophilicity, and high water absorption capability. Therefore, an attempt was made in the current study to impede the hydrolytic degradation for these materials through imparting superhydrophobicity to their surfaces. The used terpolymer has been shown to have promising potential applications as bio-absorbable surgical sutures and other biomedical materials, and thus, its applicability could be further extended upon impeding its hydrolytic degradation. Moreover, a novel method including combined use of non-solvent and nanoparticles was utilized to achieve superhydrophobicity. Very diverse wettability results were obtained which were attributed to the obtained various morphologies according to scanning electron microscopy results. More importantly, a unique hierarchical morphology was found to be responsible for the observed water repellent behavior. X-ray photoelectron spectroscopy results revealed co-existence of nanosilica particles and terpolymer chains on the surface's top layer. Finally, it was found that the superhydrophobic sample exhibited a significantly impeded hydrolytic degradation as compared with the hydrophilic pure terpolymer which was attributed to the formation of air pockets on the surface's top layer.

  5. Surface modification of traditional and bioresorbable metallic implant materials for improved biocompatibility

    Science.gov (United States)

    Walker, Emily K.

    Due to their strength, elasticity, and durability, a variety of metal alloys are commonly used in medical implants. Traditionally, corrosion-resistant metals have been preferred. These permanent materials can cause negative systemic and local tissue effects in the long-term. Permanent stenting can lead to late-stent thrombosis and in-stent restenosis. Metallic pins and screws for fracture fixation can corrode and fail, cause loss of bone mass, and contribute to inflammation and pain at the implant site, requiring reintervention. Corrodible metallic implants have the potential to prevent many of these complications by providing transient support to the affected tissue, dissolving at a rate congruent with the healing of the tissue. Alloys of iron and manganese (FeMn) exhibit similar fatigue strength, toughness, and elasticity compared with 316L stainless steel, making them very attractive candidates for bioresorbable stents and temporary fracture fixation devices. Much attention in recent years has been given to creating alloys with ideal mechanical properties for various applications. Little work has been done on determining the blood compatibility of these materials or on examining how their surfaces can be improved to improve cell adhesion, however. We examined thethrombogenic response of blood exposed to various resorbable ferrous stent materials through contact with porcine blood. The resorbable materials induced comparable or lower levels of several coagulation factors compared with 316L stainless steel. Little platelet adhesion was observed on any of the tested materials. Endothelialization is an important process after the implantation of a vascular stent, as it prevents damage to the vessel wall that can accelerate neointimal hyperplasia. Micromotion can lead to the formation of fibrous tissue surrounding an orthopedic implant, loosening, and ultimately failure of the implant. Nanoscale features were created on the surfaces of noble metal coatings, silicon

  6. Improve the physical and chemical properties of biocompatible polymer material by MeV He ion beam

    International Nuclear Information System (INIS)

    There is a high interest in improving the hydrophilicity of polymer surfaces due to their wide use for technological purposes. In this study Ultra High Molecular Weight Polyethylene (UHMWPE) as a biocompatible material was bombarded with 1 MeV He ions to the fluences ranging from 1×1013 to 5×1014 cm−2. The pristine and ion beam modified samples were investigated by photoluminescence (PL), ultraviolet–visible (UV–vis) spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The changes of wettability and surface free energy were determined by the contact angle measurements. The obtained results showed that the ion bombardment induced decrease in integrated luminescence intensity and decrease in the transmittance with increase of ion fluence as well. This is might be attributed to degradation of polymer surface and/or creation of new electronic levels in the forbidden gap. The FTIR spectral studies indicate that the ion beam induces chemical modifications within the bombarded UHMWPE. Formation of carbonyl groups (C=O) on the polymer surface was studied. Direct relationship of the wettability and surface free energy of the bombarded polymer with the ion fluences was observed. - Highlights: ► Effect of 1 MeV He-ion bombardment on the surface properties of UHMWPE was studied. ► Change in the surface layer composition of UHMWPE due ion beam has been investigated. ► Ion beam bombardment improves the surface free energy of UHMWPE.

  7. Improve the physical and chemical properties of biocompatible polymer material by MeV He ion beam

    Science.gov (United States)

    Abdul-Kader, A. M.; El-Gendy, Y. A.; Al-Rashdy, Awad A.

    2012-07-01

    There is a high interest in improving the hydrophilicity of polymer surfaces due to their wide use for technological purposes. In this study Ultra High Molecular Weight Polyethylene (UHMWPE) as a biocompatible material was bombarded with 1 MeV He ions to the fluences ranging from 1×1013 to 5×1014 cm-2. The pristine and ion beam modified samples were investigated by photoluminescence (PL), ultraviolet-visible (UV-vis) spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The changes of wettability and surface free energy were determined by the contact angle measurements. The obtained results showed that the ion bombardment induced decrease in integrated luminescence intensity and decrease in the transmittance with increase of ion fluence as well. This is might be attributed to degradation of polymer surface and/or creation of new electronic levels in the forbidden gap. The FTIR spectral studies indicate that the ion beam induces chemical modifications within the bombarded UHMWPE. Formation of carbonyl groups (C=O) on the polymer surface was studied. Direct relationship of the wettability and surface free energy of the bombarded polymer with the ion fluences was observed.

  8. PCR biocompatibility of lab-on-a-chip and MEMS materials

    Science.gov (United States)

    Christensen, T. B.; Pedersen, C. M.; Gröndahl, K. G.; Jensen, T. G.; Sekulovic, A.; Bang, D. D.; Wolff, A.

    2007-08-01

    DNA amplification using the polymerase chain reaction (PCR) is an important tool in biotechnology, pathogen surveillance in food, medical and forensic science etc. The PCR technique is now an important part of the research in and development of miniaturized biochemical analysis systems. However, reduced or no DNA amplification at all is an important challenge for microfabricated PCR devices due to a negative interaction between PCR chemicals and the surrounding environment, i.e. the materials encapsulating the PCR mix. Materials of special interest regarding PCR compatibility are silicon, glass and polymers, which are important in the fabrication of microelectromechanical systems (MEMS), micro total analysis systems (µTAS) and lab-on-a-chip (LOC) systems. The PCR inhibition effect is a particularly important phenomenon in microsystems due to an increased surface-to-volume ratio which enhances the possibility of interaction between the surfaces and ingredients in the PCR mixture. By proper surface treatment the PCR reaction can be facilitated and in this paper we present a systematic and quantitative study of the impact on the PCR compatibility of a chemical and a biological surface treatment. The chemical treatments are based on the silanizing agent dichlordimethylsilane [(CH3)2SiCl2

  9. Biodeuterated microbial chitosan for characterisation by neutron scattering and development of new biocompatible materials

    International Nuclear Information System (INIS)

    Chitosan is one of the most abundant natural polysaccharides on earth and has found a wide range of applications in biomedical and environmental fields. The most common source of commercially available chitosan is produced through the deacetylation of chitin from crustacean (mostly shrimp) shells. However, chitosan (along with chitin) is also a major component of the cell walls of a range of fungi, which can be grown and processed under controlled conditions to influence the degree of deacetylation and molecular weight of the extracted chitosan. We have selected the single cell yeast Pichia pastoris, which can also be used for recombinant expression of biodeuterated proteins. P. pastoris was cultivated in a bioreactor using deuterated methanol (CD3OD,) as sole carbon source in the growth medium and heavy water (D20) as the solvent. NMR and mass spectrometry analysis demonstrated the complete deuteration of the non-exchangeable protons in the extracted chitosan molecule. Further tuning of the level of deuteration may be achieved by changing the H/D content in the growth medium. Apart from limited reports of partially deuterated chitosan through functional groups or exchangeable protons using deuterated solvent, there are no examples of biosynthesised deuterated chitosan in the literature. This capability, developed at the National Deuteration Facility, ANSTO, provides a range of possibilities for characterising chitosan materials using isotope-sensitive techniques.

  10. Ex vivo and in vitro synchrotron-based micro-imaging of biocompatible materials applied in dental surgery

    Science.gov (United States)

    Rack, A.; Stiller, M.; Nelson, K.; Knabe, C.; Rack, T.; Zabler, S.; Dalügge, O.; Riesemeier, H.; Cecilia, A.; Goebbels, J.

    2010-09-01

    Biocompatible materials such as porous bioactive calcium phosphate ceramics or titanium are regularly applied in dental surgery: ceramics are used to support the local bone regeneration in a given defect, afterwards titanium implants replace lost teeth. The current gold standard for bone reconstruction in implant dentistry is the use of autogenous bone grafts. But the concept of guided bone regeneration (GBR) has become a predictable and well documented surgical approach using biomaterials (bioactive calcium phosphate ceramics) which qualify as bone substitutes for this kind of application as well. We applied high resolution synchrotron microtomography and subsequent 3d image analysis in order to investigate bone formation and degradation of the bone substitute material in a three-dimensional manner, extending the knowledge beyond the limits of classical histology. Following the bone regeneration, titanium-based implants to replace lost teeth call for high mechanical precision, especially when two-piece concepts are used in order to guaranty leak tightness. Here, synchrotron-based radiography in comparison with classical laboratory radiography yields high spatial resolution in combination with high contrast even when exploiting micro-sized features in these kind of highly attenuating objects. Therefore, we could study micro-gap formation at interfaces in two-piece dental implants with the specimen under different mechanical load. We could prove the existence of micro-gaps for implants with conical connections as well as to study the micromechanical behavior of the mating zone of conical implants during loading. The micro-gap is a potential issue of failure, i. e. bacterial leakage which can induce an inflammatory process.

  11. Phase III study on surface construction and biocompatibility of polymer materials as cardiovascular devices:coagulant and anti-coagulant surface modification

    Institute of Scientific and Technical Information of China (English)

    Chen Bao-lin; Wang Dong-an

    2015-01-01

    BACKGROUND: As the cardiovascular device, biomaterials applied under the blood-contact conditions should have anti-thrombotic, anti-biodegradable and anti-infective properties. OBJECTIVE: To develop novel polymer materials for implantation and intervention in cardiovascular tissue engineering and to explore the biocompatibility, blood compatibility and cytocompatibility of the surface-modified polymer biomaterials based on the coagulant and anti-coagulant coating modification. METHODS:We retrieved PubMed and WanFang databases for relevant articles publishing from 1983 to 2014. The key words were "biocompatibility, blood compatibility, biomedical materials, biomedical polymer materials" in English and Chinese, respectively. Those unrelated, outdated and repetitive papers were excluded. Literatures addressing the blood compatibility of biomedical polymer materials were summarized. RESULTS AND CONCLUSION: The blood-implant interaction and the anti-coagulant surface modification of biomaterials were analyzed. The biocompatibility, blood compatibility and cytocompatibility of the surface-modified polymer biomaterials were determined based on the coagulant and anti-coagulant coating modification. The coagulant and anti-coagulant surface modification of polymer biomaterials and the research on their biocompatibility and endothelial cel compatibility are crucial for developing novel polymer materials for implantation and intervention in cardiovascular tissue engineering. Through in-depth studies of the types and applications of polymer biomaterials, cardiovascular medical devices and implantable soft tissue substitutes, the differences between the surface and the body wil be reflected in the many layers of molecules extending from the surface to the body. Two major factors, surface energy and molecular mobility, determine the body/surface behaviors that include body/surface differences and phase separation. Considering the difference between the body/surface composition

  12. Biocompatibility of composite resins

    OpenAIRE

    Sayed Mostafa Mousavinasab

    2011-01-01

    Dental materials that are used in dentistry should be harmless to oral tissues, so they should not contain any leachable toxic and diffusible substances that can cause some side effects. Reports about probable biologic hazards, in relation to dental resins, have increased interest to this topic in dentists. The present paper reviews the articles published about biocompatibility of resin-restorative materials specially resin composites and monomers which are mainly based on Bis-GMA and concern...

  13. Influence of the polymer amount on bioactivity and biocompatibility of SiO{sub 2}/PEG hybrid materials synthesized by sol–gel technique

    Energy Technology Data Exchange (ETDEWEB)

    Catauro, M., E-mail: michelina.catauro@unina2.it [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Bollino, F.; Papale, F. [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa (Italy); Gallicchio, M.; Pacifico, S. [Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100 Caserta (Italy)

    2015-03-01

    SiO{sub 2}/PEG organic–inorganic hybrid materials, which differ in polyethylene glycol (PEG) content, were synthesized by sol–gel technique and the characterization of their structure and biological properties was carried out in order to evaluate the possible use in biomedical field. FT-IR spectroscopy detected that the two components of the hybrids (SiO{sub 2} and PEG) are linked by hydrogen bonds between the Si–OH groups of the inorganic phase and the terminal alcoholic groups and/or the ethereal oxygen atoms in the repeating units of polymer. X-ray diffraction analysis ascertained the amorphous nature of the gels and the observation of their morphology by SEM microscopy confirmed that the interpenetration of the two phases (organic and inorganic) occurs on nanometric scale. The biological characterization was carried out as a function of the polymer amount to study its influence on material behavior. The results showed that the synthesized materials were bioactive and biocompatible. The formation of a hydroxyapatite layer, indeed, was observed on their surface by SEM/EDX analysis after soaking in simulated body fluid. Moreover, the biocompatibility of SiO{sub 2}/PEG hybrids was assessed performing MTT and SRB cytotoxicity tests on fibroblast cell NIH 3T3 after 24 and 48 h of exposure, as well as Trypan Blue dye exclusion test. The response to the presence of the investigated materials was positive. The cell growth and proliferation showed dependence on polymer amount and time of exposure to the material extracts. Therefore, the obtained results are encouraging for the use of the obtained hybrids in dental or orthopedic applications. - Highlights: • SiO{sub 2}/PEG hybrid biomaterials synthesized by sol–gel method at various PEG percentages • Chemical and morphological characterization of hybrid materials • Chemical interactions between inorganic and organic components • Biological characterizations with MTT and SRB cytotoxicity tests

  14. Influence of the polymer amount on bioactivity and biocompatibility of SiO2/PEG hybrid materials synthesized by sol–gel technique

    International Nuclear Information System (INIS)

    SiO2/PEG organic–inorganic hybrid materials, which differ in polyethylene glycol (PEG) content, were synthesized by sol–gel technique and the characterization of their structure and biological properties was carried out in order to evaluate the possible use in biomedical field. FT-IR spectroscopy detected that the two components of the hybrids (SiO2 and PEG) are linked by hydrogen bonds between the Si–OH groups of the inorganic phase and the terminal alcoholic groups and/or the ethereal oxygen atoms in the repeating units of polymer. X-ray diffraction analysis ascertained the amorphous nature of the gels and the observation of their morphology by SEM microscopy confirmed that the interpenetration of the two phases (organic and inorganic) occurs on nanometric scale. The biological characterization was carried out as a function of the polymer amount to study its influence on material behavior. The results showed that the synthesized materials were bioactive and biocompatible. The formation of a hydroxyapatite layer, indeed, was observed on their surface by SEM/EDX analysis after soaking in simulated body fluid. Moreover, the biocompatibility of SiO2/PEG hybrids was assessed performing MTT and SRB cytotoxicity tests on fibroblast cell NIH 3T3 after 24 and 48 h of exposure, as well as Trypan Blue dye exclusion test. The response to the presence of the investigated materials was positive. The cell growth and proliferation showed dependence on polymer amount and time of exposure to the material extracts. Therefore, the obtained results are encouraging for the use of the obtained hybrids in dental or orthopedic applications. - Highlights: • SiO2/PEG hybrid biomaterials synthesized by sol–gel method at various PEG percentages • Chemical and morphological characterization of hybrid materials • Chemical interactions between inorganic and organic components • Biological characterizations with MTT and SRB cytotoxicity tests • Biocompatibility improvement with

  15. Biocompatibility of Dental Amalgams

    OpenAIRE

    Yurdanur Uçar; Brantley, William A.

    2011-01-01

    Objective. The purpose of this review paper is to review the literature regarding the toxicology of mercury from dental amalgam and evaluate current statements on dental amalgam. Materials and Methods. Two key-words “dental amalgam” and “toxicity” were used to search publications on dental amalgam biocompatibility published in peer-reviewed journals written in English. Manual search was also conducted. The most recent declarations and statements were evaluated using information available on ...

  16. Electrospinning of Biodegradable and Biocompatible Nanofiber Patches from Solutions of ``Green'' Materials for Plant Protection against Fungi Attack

    Science.gov (United States)

    Sett, Soumyadip; Lee, Minwook; Yarin, Alexander; Moghadam, S. M. Alavi; Meinke, Matthias; Schroeder, Wolfgang

    2015-11-01

    Biodegradable and biocompatible soy protein/petroleum-derived polymer monolithic fibers containing adhesives were electrospun on commercial rayon pads. The polymers used, PVA and PCL, are widely used in the biomedical industry, including such applications as drug delivery and scaffold manufacturing. Soy protein is an abundant waste of SoyDiesel production, and is widely used as a nutrient. The soy content in our fibers was as high as 40% w/w. Four different adhesives, including ordinary wood glue, repositionable glue and FDA-approved pressure-sensitive glue were used for electrospinning and electrospraying. The normal and shear adhesive strengths of the patches developed in this work were measured and compared. The adhesive strength was sufficient enough to withstand normal atmospheric conditions. These biodegradable and biocompatible nano-textured patches are ready to be used on prune locations without being carried away by wind and will protect plants against fungi attack at these locations, preventing diseases like Vine Decline.

  17. Coatings of titanium substrates with xCaO · (1 - x)SiO2 sol-gel materials: characterization, bioactivity and biocompatibility evaluation.

    Science.gov (United States)

    Catauro, M; Papale, F; Bollino, F

    2016-01-01

    The objective of this study has been to develop low temperature sol-gel coatings to modify the surface of commercially pure titanium grade 4 (a material generally used in dental application) and to evaluate their bioactivity and biocompatibility on the substrate. Glasses of composition expressed by the following general formula xCaO · (1 - x)SiO2 (0.0characterized and a microstructural analysis of the coatings obtained was performed using scanning electron microscopy (SEM). The potential applications of the coatings in the biomedical field were evaluated by bioactivity and biocompatibility tests. The coated titanium was immersed in simulated body fluid (SBF) for 21 days and the hydroxyapatite deposition on its surface was subsequently evaluated via SEM-EDXS analysis, as an index of bone-bonding capability. To investigate cell-material interactions, mouse embryonic fibroblast cells (3T3) were seeded onto the specimens and the cell viability was evaluated by a WST-8 assay. PMID:26478379

  18. Fiscal 1998 regional consortium R and D project (Regional consortium field). Report on R and D of production technology of hybrid-type biocompatible hard tissue replacing materials (1st fiscal year); 1998 nendo chiiki konsoshiamu kenkyu kaihatsu jigyo. Chiiki konsoshiamu bun'ya (hybrid gata seitai yugo kinosei kososhiki daitai sozai seizo gijutsu no kenkyu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    This project aims at development of high-strength high- biocompatible {beta}-type Ti alloy with lower modulli of elasticity composed of non-toxic elements, phosphate calcium ceramics for improving the biocompatibility and coating technology, and establishment of production technology of hybrid-type biocompatible hard tissue replacing materials. In fiscal 1998, the project promoted the following: Design of high-biocompatible {beta}-type Ti alloy materials, development of thermomechanical treatment for improving dynamic characteristics of such alloy, survey on practical melting and casting technologies and dental precision casting process, evaluation of the biocompatibility of the alloy by cytotoxicity, selection of tools for precision machining and surface finishing, control of contents, orientation and precipitation of biocompatible crystals such as {beta}- Ca(PO{sub 3}){sub 2} fibers for further improvement of the biocompatibility. This paper also outlines the survey results on the market needs, market size and market share for the feasibility of these materials. (NEDO)

  19. Biocompatibility of plasma nanostructured biopolymers

    Czech Academy of Sciences Publication Activity Database

    Kasálková-Slepičková, N.; Slepička, P.; Bačáková, Lucie; Sajdl, P.; Švorčík, V.

    2013-01-01

    Roč. 307, Jul 15 (2013), s. 642-646. ISSN 0168-583X R&D Projects: GA ČR(CZ) GBP108/12/G108 Institutional support: RVO:67985823 Keywords : biopolymer * plasma treatment * biocompatibility Subject RIV: JJ - Other Materials Impact factor: 1.186, year: 2013

  20. 人角膜接触镜材料生物相容性的研究%Studies on biocompatibility of contact lens material for human cornea

    Institute of Scientific and Technical Information of China (English)

    朱文渊; 刘正堂; 崔英德; 黎新明

    2007-01-01

    Objective To study the biocompatibility of contact lens material for human cornea prepared in our department. Methods Biological properties of the material were assessed by cytotoxicity,haemolysis and protein aggradation. Results The material had no toxicity for HEFC. The haemolysis rate was eligibility(1.62%). Anti-protein-aggradation was quite good. Conclusion The contact lens material by our department possesses quite good biological properties.%目的 研究本室制备的人角膜接触镜材料的生物性能.方法 采用细胞毒性试验、红细胞溶血试验以及蛋白质沉积试验方法.结果 所制备的人角膜接触镜材料对人胚肺纤维细胞(HEFC)毒性评价为1级无毒性;红细胞溶血率为1.62%合格;在体温范围内抗蛋白质沉积作用较好.结论 本室制备的人角膜接触镜材料具有较好的生物相容性.

  1. Biocompatibility of Niobium Coatings

    Directory of Open Access Journals (Sweden)

    René Olivares-Navarrete

    2011-09-01

    Full Text Available Niobium coatings deposited by magnetron sputtering were evaluated as a possible surface modification for stainless steel (SS substrates in biomedical implants. The Nb coatings were deposited on 15 mm diameter stainless steel substrates having an average surface roughness of 2 mm. To evaluate the biocompatibility of the coatings three different in vitro tests, using human alveolar bone derived cells, were performed: cellular adhesion, proliferation and viability. Stainless steel substrates and tissue culture plastic were also studied, in order to give comparative information. No toxic response was observed for any of the surfaces, indicating that the Nb coatings act as a biocompatible, bioinert material. Cell morphology was also studied by immune-fluorescence and the results confirmed the healthy state of the cells on the Nb surface. X-ray diffraction analysis of the coating shows that the film is polycrystalline with a body centered cubic structure. The surface composition and corrosion resistance of both the substrate and the Nb coating were also studied by X-ray photoelectron spectroscopy and potentiodynamic tests. Water contact angle measurements showed that the Nb surface is more hydrophobic than the SS substrate.

  2. Biocompatible polysaccharide-based cryogels

    International Nuclear Information System (INIS)

    This study focuses on the development of novel biocompatible macroporous cryogels by electron-beam assisted free-radical crosslinking reaction of polymerizable dextran and hyaluronan derivatives. As a main advantage this straightforward approach provides highly pure materials of high porosity without using additional crosslinkers or initiators. The cryogels were characterized with regard to their morphology and their basic properties including thermal and mechanical characteristics, and swellability. It was found that the applied irradiation dose and the chemical composition strongly influence the material properties of the resulting cryogels. Preliminary cytotoxicity tests illustrate the excellent in vitro-cytocompatibility of the fabricated cryogels making them especially attractive as matrices in tissue regeneration procedures. - Graphical abstract: Electron-beam initiated synthesis of biocompatible cryogels based on natural polymers. - Highlights: • Successful electron-beam induced synthesis of dextran and hyaluronan cryogels. • Mechanical and thermal stable cryogels were obtained. • Excellent cytocompatibility of the materials was proven. • Promising materials for tissue engineering were developed

  3. Biocompatible polysaccharide-based cryogels

    Energy Technology Data Exchange (ETDEWEB)

    Reichelt, Senta, E-mail: senta.reichelt@iom-leipzig.de [Leibniz Institute of Surface Modification, Permoserstr. 15, 04318 Leipzig (Germany); Becher, Jana; Weisser, Jürgen [Innovent e.V., Pruessingstr. 27B, 07745 Jena (Germany); Prager, Andrea; Decker, Ulrich [Leibniz Institute of Surface Modification, Permoserstr. 15, 04318 Leipzig (Germany); Möller, Stephanie; Berg, Albrecht; Schnabelrauch, Matthias [Innovent e.V., Pruessingstr. 27B, 07745 Jena (Germany)

    2014-02-01

    This study focuses on the development of novel biocompatible macroporous cryogels by electron-beam assisted free-radical crosslinking reaction of polymerizable dextran and hyaluronan derivatives. As a main advantage this straightforward approach provides highly pure materials of high porosity without using additional crosslinkers or initiators. The cryogels were characterized with regard to their morphology and their basic properties including thermal and mechanical characteristics, and swellability. It was found that the applied irradiation dose and the chemical composition strongly influence the material properties of the resulting cryogels. Preliminary cytotoxicity tests illustrate the excellent in vitro-cytocompatibility of the fabricated cryogels making them especially attractive as matrices in tissue regeneration procedures. - Graphical abstract: Electron-beam initiated synthesis of biocompatible cryogels based on natural polymers. - Highlights: • Successful electron-beam induced synthesis of dextran and hyaluronan cryogels. • Mechanical and thermal stable cryogels were obtained. • Excellent cytocompatibility of the materials was proven. • Promising materials for tissue engineering were developed.

  4. On the Rule of Mixtures for Predicting Stress-Softening and Residual Strain Effects in Biological Tissues and Biocompatible Materials

    Directory of Open Access Journals (Sweden)

    Alex Elías-Zúñiga

    2014-01-01

    Full Text Available In this work, we use the rule of mixtures to develop an equivalent material model in which the total strain energy density is split into the isotropic part related to the matrix component and the anisotropic energy contribution related to the fiber effects. For the isotropic energy part, we select the amended non-Gaussian strain energy density model, while the energy fiber effects are added by considering the equivalent anisotropic volumetric fraction contribution, as well as the isotropized representation form of the eight-chain energy model that accounts for the material anisotropic effects. Furthermore, our proposed material model uses a phenomenological non-monotonous softening function that predicts stress softening effects and has an energy term, derived from the pseudo-elasticity theory, that accounts for residual strain deformations. The model’s theoretical predictions are compared with experimental data collected from human vaginal tissues, mice skin, poly(glycolide-co-caprolactone (PGC25 3-0 and polypropylene suture materials and tracheal and brain human tissues. In all cases examined here, our equivalent material model closely follows stress-softening and residual strain effects exhibited by experimental data.

  5. Bioglass: A novel biocompatible innovation

    Directory of Open Access Journals (Sweden)

    Vidya Krishnan

    2013-01-01

    Full Text Available Advancement of materials technology has been immense, especially in the past 30 years. Ceramics has not been new to dentistry. Porcelain crowns, silica fillers in composite resins, and glass ionomer cements have already been proved to be successful. Materials used in the replacement of tissues have come a long way from being inert, to compatible, and now regenerative. When hydroxyapatite was believed to be the best biocompatible replacement material, Larry Hench developed a material using silica (glass as the host material, incorporated with calcium and phosphorous to fuse broken bones. This material mimics bone material and stimulates the regrowth of new bone material. Thus, due to its biocompatibility and osteogenic capacity it came to be known as "bioactive glass-bioglass." It is now encompassed, along with synthetic hydroxyapatite, in the field of biomaterials science known as "bioactive ceramics." The aim of this article is to give a bird′s-eye view, of the various uses in dentistry, of this novel, miracle material which can bond, induce osteogenesis, and also regenerate bone.

  6. Improving biocompatibility of implants with nanolayering

    OpenAIRE

    Homar, Miha; GAŠPERLIN, MIRJANA; Dolenc, Andrej; Kristl, Julijana

    2015-01-01

    Biocompatibility of different materials in the body is very complicated phenomenon. Although structural characteristics of implant are decisive for its specific purpose, biologic response mainly depends on surface characteristics of the implant and its interaction with biologic environment. Nanolayering of the implant to improve biocompatibility presents a recent innovation in this field of research. One method of nanolayering is layer-by-layer assembly which has many advantages over other na...

  7. A biocompatible magnetic film: synthesis and characterization

    OpenAIRE

    Chatterjee, Jhunu; Haik, Yousef; Chen, Ching Jen

    2004-01-01

    Background Biotechnology applications of magnetic gels include biosensors, targeted drug delivery, artificial muscles and magnetic buckles. These gels are produced by incorporating magnetic materials in the polymer composites. Methods A biocompatible magnetic gel film has been synthesized using polyvinyl alcohol. The magnetic gel was dried to generate a biocompatible magnetic film. Nanosized iron oxide particles (γ-Fe2O3, ~7 nm) have been used to produce the magnetic gel. Results The surface ...

  8. Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™ Used as Root-End Filling Materials

    Directory of Open Access Journals (Sweden)

    Diana María Escobar-García

    2016-01-01

    Full Text Available Objective. The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA and Biodentine (BD on periodontal ligament fibroblasts (PDL. Methods. PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods. Also, the LIVE/DEAD assay was performed at 24 h. For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrin β1 and Vinculin at 12 h and 24 h. Statistical analysis was performed by the Kruskal-Wallis and Mann-Whitney U tests. Results. MTA and BD exhibited living cells up to 7 days. More expressions of Integrin β1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes. There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements. Conclusions. Neither material was cytotoxic during the time evaluated. There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.

  9. In vitro and in vivo biocompatibility and osteogenesis of graphene-reinforced nanohydroxyapatite polyamide66 ternary biocomposite as orthopedic implant material

    Science.gov (United States)

    Zhang, Shiyang; Yang, Qiming; Zhao, Weikang; Qiao, Bo; Cui, Hongwang; Fan, Jianjun; Li, Hong; Tu, Xiaolin; Jiang, Dianming

    2016-01-01

    Graphene and its derivatives have been receiving increasing attention regarding their application in bone tissue engineering because of their excellent characteristics, such as a vast specific surface area and excellent mechanical properties. In this study, graphene-reinforced nanohydroxyapatite/polyamide66 (nHA/PA66) bone screws were prepared. The results of scanning electron microscopy observation and X-ray diffraction data showed that both graphene and nHA had good dispersion in the PA66 matrix. In addition, the tensile strength and elastic modulus of the composites were significantly improved by 49.14% and 21.2%, respectively. The murine bone marrow mesenchymal stem cell line C3H10T1/2 exhibited better adhesion and proliferation in graphene reinforced nHA/PA66 composite material compared to the nHA/PA66 composites. The cells developed more pseudopods, with greater cell density and a more distinguishable cytoskeletal structure. These results were confirmed by fluorescent staining and cell viability assays. After C3H10T1/2 cells were cultured in osteogenic differentiation medium for 7 and 14 days, the bone differentiation-related gene expression, alkaline phosphatase, and osteocalcin were significantly increased in the cells cocultured with graphene reinforced nHA/PA66. This result demonstrated the bone-inducing characteristics of this composite material, a finding that was further supported by alizarin red staining results. In addition, graphene reinforced nHA/PA66 bone screws were implanted in canine femoral condyles, and postoperative histology revealed no obvious damage to the liver, spleen, kidneys, brain, or other major organs. The bone tissue around the implant grew well and was directly connected to the implant. The soft tissues showed no obvious inflammatory reaction, which demonstrated the good biocompatibility of the screws. These observations indicate that graphene-reinforced nHA/PA66 composites have great potential for application in bone tissue

  10. Biocompatibility of different post-core materials in molar repairing%不同桩核修复磨牙的生物相容性比较

    Institute of Scientific and Technical Information of China (English)

    蔡娟; 郁章欣; 吴金枝; 刘新庆

    2013-01-01

      背景:磨牙是主要的功能牙齿,磨牙的修复应能改善其生物功能和具有坚固的组织学效果。目的:评价不同桩核材料在磨牙修复中的应用效果。方法:对应用银汞桩核、铸造桩核和纤维桩核修复磨牙缺损的患者进行随访观察,检查修复体松动、折断、脱落以及牙周炎和牙龈炎等发生情况,X 射线检查桩核冠修复体与牙体间的结合情况以及根管折断情况和根尖根周牙槽骨吸收情况,评价不同桩核材料修复磨牙的生物相容性并进行比较。结果与结论:银汞桩核、铸造桩核和纤维桩核修复磨牙均可以获得较好的治疗效果,但是,与铸造桩核相比较,银汞桩核和纤维桩核的生物性能更好,修复体松动、折断和脱落的发生率更低,根尖根周等炎性并发症的发生率也更低,并且组织学坚固效果更好,是磨牙修复中首选的桩核材料。%BACKGROUND: Molars are the main functional teeth, and molar repairing is able to improve the biological function of molars and has the sturdy histological effect. OBJECTIVE: To evaluate the application effect of different post-core materials in molar repairing. METHODS: The patients received molar repairing with amalgam post-core, cast post-core and fiber post-core were fol owed-up to observe the incidence of prosthesis loosening, fracture, loss, periodontitis and gingivitis. The combination between post-core crown prosthesis and dental body, broken root canal and the resorption of alveolar bone of root tip and root body were tested with X-ray film. The biocompatibility of different post-core materials used for molar repairing was evaluated and compared.RESULTS AND CONCLUSION: The amalgam post-core, cast post-core and fiber post-core have good effects for the repair of molars. But compared with cast post-core, the amalgam post-core and fiber post-core have better biocompatibility, lower incidence of prosthesis

  11. Biocompatibility of polyaniline

    Czech Academy of Sciences Publication Activity Database

    Humpolíček, P.; Kašpárková, V.; Saha, P.; Stejskal, Jaroslav

    2012-01-01

    Roč. 162, 7/8 (2012), s. 722-727. ISSN 0379-6779 R&D Projects: GA ČR GA202/09/1626 Institutional research plan: CEZ:AV0Z40500505 Keywords : conducting polymer * polyaniline * biocompatibility Subject RIV: BK - Fluid Dynamics Impact factor: 2.109, year: 2012

  12. Evaluation of the Biocompatibility of Poly (ortho ester), Copolymer of ε-caprolactone/D,L-lactide and the Composite of Copolymer of ε-caprolactone/D,L-lactide and Tricalciumphosphate as Bone Filling Material

    OpenAIRE

    Ekholm, Marja

    2006-01-01

    The purpose of this series of studies was to evaluate the biocompatibility of poly (ortho) ester (POE), copolymer of ε-caprolactone and D,L-lactide [P (ε-CL/DL-LA)] and the composite of P(ε-CL/DL-LA) and tricalciumphosphate (TCP) as bone filling material in bone defects. Tissue reactions and resorption times of two solid POE-implants (POE 140 and POE 46) with different methods of sterilization (gamma- and ethylene oxide sterilization), P(ε-CL/DL-LA)(40/60 w/w) in paste form and 50/50 w/w ...

  13. Biocompatible composites of ultrahigh molecular weight polyethylene

    Science.gov (United States)

    Panin, S. V.; Kornienko, L. A.; Suan, T. Nguen; Ivanova, L. P.; Korchagin, M. A.; Chaikina, M. V.; Shilko, S. V.; Pleskachevskiy, Yu. M.

    2015-10-01

    Mechanical and tribotechnical characteristics of biocompatible, antifriction and extrudable composites based on ultrahigh molecular weight polyethylene (UHMWPE) as well as hybrid matrix "UHMWPE + PTFE" with biocompatible hydroxyapatite filler under the dry friction and boundary lubrication were investigated. A comparative analysis of effectiveness of adding the hydroxyapatite to improve the wear resistance of composites based on these two matrices was performed. It is shown that the wear intensity of nanocomposites based on the hybrid matrix is lower than that for the composites based on pure UHMWPE. Possibilities of using the composites of the polymer "UHMWPE-PTFE" mixture as a material for artificial joints implants are discussed.

  14. The Biocompatibility of Wool Keratin

    Institute of Scientific and Technical Information of China (English)

    LIU Mei; YU Wei-dong; WANG Xue-lei

    2007-01-01

    Keratin is the major structural fibrous protein providing outer covering such as hair, wool, feathers, etc. When being used as a kind of biomaterials, the biocompatibility of wool keratin is one of the most critical questions. By now, there has not been systemic study on the biocompatibiiity of keratin. Therefore, in this article we used the procedures of skin irritation, haemolysis and subcutaneous implantation according to ISO 10993 to study it. Moreover, the Fourier transform-infrared (FTIR) spectroscopy was utilized to analyse the impurity and structure modification of wool keratin film. The part of the animal tests showed that the wool keratin films prepared by authors were biocompatible. But the residual of sodium dodecyi sulfate (SDS) affected the results of other tests. Consequently, the wool keratin membrane is one kind of favourable and promising biomaterial for biomedical and histological utilization. The residual SDS used as an agent should be eliminated from the keratin solution or membrane completely if for biological usage. In conclusion, wool keratin, as a kind of natural protein, prospectively could be applied in biomedical materials and scaffolds of tissue engineering.

  15. Biocompatibility of plasma nanostructured biopolymers

    Energy Technology Data Exchange (ETDEWEB)

    Slepičková Kasálková, N. [Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague (Czech Republic); Slepička, P., E-mail: petr.slepicka@vscht.cz [Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague (Czech Republic); Bačáková, L. [Institute of Physiology, Academy of Sciences of the Czech Republic 142 20 Prague (Czech Republic); Sajdl, P. [Department of Power Engineering, Institute of Chemical Technology, 166 28 Prague (Czech Republic); Švorčík, V. [Department of Solid State Engineering, Institute of Chemical Technology, 166 28 Prague (Czech Republic)

    2013-07-15

    Many areas of medicine such as tissue engineering requires not only mastery of modification techniques but also thorough knowledge of the interaction of cells with solid state substrates. Plasma treatment can be used to effective modification, nanostructuring and therefore can significantly change properties of materials. In this work the biocompatibility of the plasma nanostructured biopolymers substrates was studied. Changes in surface chemical structure were studied by X-ray photoelectron spectroscopy (XPS). The morphology pristine and modified samples were determined using atomic force microscopy (AFM). The surface wettability was determined by goniometry from contact angle. Biocompatibility was determined by in vitro tests, the rat vascular smooth muscle cells (VSMCs) were cultivated on the pristine and plasma modified biopolymer substrates. Their adhesion, proliferation, spreading and homogeneous distribution on polymers was monitored. It was found that the plasma treatment leads to rapid decrease of contact angle for all samples. Contact angle decreased with increasing time of modification. XPS measurements showed that plasma treatment leads to changes in ratio of polar and non-polar groups. Plasma modification was accompanied by a change of surface morphology. Biological tests found that plasma treatment have positive effect on cells adhesion and proliferation cells and affects the size of cell’s adhesion area. Changes in plasma power or in exposure time influences the number of adhered and proliferated cells and their distribution on biopolymer surface.

  16. Biocompatibility of plasma nanostructured biopolymers

    International Nuclear Information System (INIS)

    Many areas of medicine such as tissue engineering requires not only mastery of modification techniques but also thorough knowledge of the interaction of cells with solid state substrates. Plasma treatment can be used to effective modification, nanostructuring and therefore can significantly change properties of materials. In this work the biocompatibility of the plasma nanostructured biopolymers substrates was studied. Changes in surface chemical structure were studied by X-ray photoelectron spectroscopy (XPS). The morphology pristine and modified samples were determined using atomic force microscopy (AFM). The surface wettability was determined by goniometry from contact angle. Biocompatibility was determined by in vitro tests, the rat vascular smooth muscle cells (VSMCs) were cultivated on the pristine and plasma modified biopolymer substrates. Their adhesion, proliferation, spreading and homogeneous distribution on polymers was monitored. It was found that the plasma treatment leads to rapid decrease of contact angle for all samples. Contact angle decreased with increasing time of modification. XPS measurements showed that plasma treatment leads to changes in ratio of polar and non-polar groups. Plasma modification was accompanied by a change of surface morphology. Biological tests found that plasma treatment have positive effect on cells adhesion and proliferation cells and affects the size of cell’s adhesion area. Changes in plasma power or in exposure time influences the number of adhered and proliferated cells and their distribution on biopolymer surface

  17. Biocompatibility of polyaniline

    Czech Academy of Sciences Publication Activity Database

    Humpolíček, P.; Kašpárková, V.; Stejskal, Jaroslav; Sáha, P.; Kuceková, Z.

    Prague : Institute of Macromolecular Chemistry AS CR, 2011. s. 129. ISBN 978-80-85009-69-9. [Prague Meeting on Macromolecules /75./ - Conducting Polymers - Formation, Structure, Properties, and Applications. 10.07.2011-14.07.2011, Prague] R&D Projects: GA ČR GA202/09/1626 Institutional research plan: CEZ:AV0Z40500505 Keywords : polyaniline * biocompatibility Subject RIV: BK - Fluid Dynamics

  18. Biocompatibility of Niobium Coatings

    OpenAIRE

    René Olivares-Navarrete; Jhon Jairo Olaya; Claudia Ramírez; Sandra Elizabeth Rodil

    2011-01-01

    Niobium coatings deposited by magnetron sputtering were evaluated as a possible surface modification for stainless steel (SS) substrates in biomedical implants. The Nb coatings were deposited on 15 mm diameter stainless steel substrates having an average surface roughness of 2 mm. To evaluate the biocompatibility of the coatings three different in vitro tests, using human alveolar bone derived cells, were performed: cellular adhesion, proliferation and viability. Stainles...

  19. Comparação de métodos para testar a citotoxicidade "in vitro" de materiais biocompatíveis Comparison of methods to test an "in vitro" test of cytotoxicity of biocompatible hospital materials

    Directory of Open Access Journals (Sweden)

    Aurea Silveira Cruz

    1998-04-01

    Full Text Available OBJETIVO: Comparar a sensibilidade do método de difusão em ágar e do método de extração utilizando as linhagens celulares RC-IAL (células fibroblásticas de rim de coelho e HeLa (células epiteliais de carcinoma do colo do útero humano, na avaliação da citotoxicidade "in vitro" de materiais de uso médico-hospitalar. MATERIAL E MÉTODO: Foram testadas 50 amostras escolhidas por sorteio, entre as já conhecidamente positivas e negativas e identificadas como: algodão, espuma, borracha, látex, celulose e acrílico. Além, das amostras citadas foram testadas experimentalmente várias concentrações de SDS (duodecil sulfato de sódio nas culturas celulares RC-IAL e HeLa. RESULTADOS: Das 50 amostras testadas , 44 (88% foram positivas para os dois métodos. Mas quando comparado o SDS nos dois métodos foram observados resultados positivos nas concentrações de 0,5 a 0,05 µg/ml no método de difusão em ágar e no método de extração somente foi observado efeito citotóxico até a concentração de 0,25 µg/ml. CONCLUSÃO: Os resultados encontrados são similares aos observados por outros autores que testaram materiais como, por exemplo, ligas metálicas. Quando foi usado o SDS observou-se, nas duas linhagens celulares, diferenças favoráveis ao método de difusão em ágar em duas concentrações, isto é, a sensibilidade deste método foi significantemente maior, por inspecção, em relação ao método de extração, além de se constituir em método mais simples de ser realizado.OBJECTIVE: A comparison of the sensitivity of the agar diffusion method with that of extraction using cell-lines RC-IAL (fibroblastic of rabbit kidney and HeLa (epithelial carcionoma cells from the cervix uteri of the humam uterus, in the in vitro evaluation of materials of medical and hospital. MATERIAL AND METHODS: Fifteen samples chosen at random, from among the already known positives and negatives in our stock, were tested and identified as cotton

  20. 生物珊瑚人工骨支架材料生物相容性检测%Biocompatibility test of biological coral artificial bone as scaffold materials

    Institute of Scientific and Technical Information of China (English)

    黄涛; 孟志斌; 金大地; 付昆; 刘建航; 宋策; 贾丙申

    2012-01-01

    目的 评价生物珊瑚人工骨( BCAB)材料作为骨组织工程支架材料与小鼠胚胎干细胞(MESCs)构建组织工程骨的有效性及材料生物相容性.方法 设MESCs与BCAB支架材料混合黏附培养为实验组,单纯MESCs培养为对照组,分别于第2、4、6、8天进行MTT法检测细胞增殖活性,特异性胚胎抗原-1检测细胞对材料的黏附性.于第8日对接种细胞材料片行成骨诱导,诱导培养10d后行茜素红染色及电镜扫描检测成骨诱导及体外组织工程骨构建情况.取12只大鼠,脊柱左侧皮下植入空白BCAB支架片状材料,右侧植入黏附细胞BCAB片状材料,随机分4、8、12周3组行影像学检查,并取双侧标本行病理切片观察局部炎症反应,四环素标记下荧光显微镜观察成骨情况.第12周组取心、肝、肾病理切片及评估心、肝、肾毒性反应.结果 MTT法检测细胞增殖活性结果显示,在培养2d和4d时实验组与对照组间MTT值差异无统计学意义(P>0.05),在6d及8d时实验组MTT值明显高于对照组,差异具有统计学意义(P<0.05).特异性胚胎抗原-1检测证实MESCs对BCAB支架材料具有良好的黏附性,在其三维微孔隙内能较快增殖.茜素红染色及电镜扫描检测证实黏附细胞材料成骨诱导有效,体外组织工程骨构建成功.材料植入局部组织炎症反应轻,空白支架材料于第8周开始降解,12周达初步降解,无异位成骨;黏附细胞支架材料则有明显异位成骨现象,且较对侧空白支架材料降解时效延长.第12周组实验动物心、肝、肾标本病理切片未见异常损害.结论 BCAB支架材料具有良好的生物相容性,其降解周期与新骨重建周期大致相当,是一种良好的骨组织工程支架材料.%[Objective] To test the biocompatibility of biological coral artificial bone (BCAB) and the efrect of tissue engineered bone construction using BCAB scaffolds with mouse embryonic stem cells (MESCs

  1. Vectorization of copper complexes via biocompatible and biodegradable PLGA nanoparticles

    International Nuclear Information System (INIS)

    A double emulsion-solvent diffusion approach with fully biocompatible materials was used to encapsulate copper complexes within biodegradable nanoparticles, for which the release kinetics profiles have highlighted their potential use for a prolonged circulating administration.

  2. 镍铬合金材料与口腔软组织的生物相容性%Biocompatibility of Ni-Cr alloy materials and oral soft tissue

    Institute of Scientific and Technical Information of China (English)

    金芮竹; 赵驰

    2012-01-01

    BACKGROUND: Ni-Cr alloy ceramic materials are accepted by the majority of patients due to the low prices, and more than 50% of patients choose the Ni-Cr alloy porcelain teeth. Due to the materials persisting in the electrolyte environment, precipitation of nickel ions can cause allergy in some patients. How to improve the biological safety of Ni-Cr alloy materials arises more attention. OBJECTIVE: Using CNKI database literature search and the depth of analysis capabilities, to explore the literature data trends in the study of biocompatibility of Ni-Cr alloy materials and oral soft tissue. DESIGN: Bibliometric data analysis.DATA RETRIEVAL: A search of related literature of biocompatibility of Ni-Cr alloy materials and oral soft tissue was performed in CNKI database using the key words of "Ni-Cr alloy", "biomaterials" and "biocompatibility", during 2002-01 to 2011-12. Own database analysis capabilities and Excel charting functions were used to retrieve literature analysis; through the form of text and charts, the data were analyzed to describe the distribution characteristics.SELECTION CRITERIA: Inclusive criteria: ?Basic research papers related to Ni-Cr alloy materials. ?Papers related to the clinical application of Ni-Cr alloy materials. ?Research papers related to biocompatibility of Ni-Cr alloy materials. Exclusive criteria: ?Literature has nothing to do with the purpose of this review. ?Duplication of research literature. ?Journal's own information. ?Unpublished papers. ?The article need telephone follow up to analyze and manual searches. ?Year book. MAIN OUTCOME MEASUREMENTS: In CNKI database, academic journal articles published year, literature number, subject category, research institutions, source journals, literature citations, literature download frequency, associated literature, distribution of the author, distribution of the fund and major keywords were analyzed, the Ph.D. Thesis, outstanding master's degree papers, conference papers, patented

  3. Biocompatibility and Bioactivity of Magnesium Matrix Hydroxyapatite Material%镁合金基HA材料生物相容性及生物活性的研究

    Institute of Scientific and Technical Information of China (English)

    林国湘; 薛顺; 李林升; 葛丽婧

    2015-01-01

    Magnesium matrix hydroxyapatite biological composite material was prepared by electrophoretic deposition method, and the biocompatibility and biological activity of this material was discussed. Animal experiment and SBF immersion experiment were selected separately to test the biocompatibility and bioactivity. The SD rats were divided into con-trast group and implant group. The implant material surrounding tissue was taken to do Tis-sue biopsy,HE dyed and organizational analysis after a certain amount of time in the SD body. Under homeothermic condition,the biological composite material was soaked in SBF solution. After four weeks,the bioactivity of the biological composite material was evaluated by testing the growth ability of hydroxyapatite on composite material. The experiment re-sults showed that the implant material surrounding appeared tissue hyperplasia,connective tissue and new blood vessels,but no obvious tissue inflammation,no infiltrating inflammato-ry cells such as multinuclear giant cells and neutrophils cells. After the biological compos-ite material was soaked in SBF solution,a layer Bone-Like Apatite was found on its sur-face. The results showed that Magnesium matrix hydroxyapatite biological composite materi-al have no cytotoxicity,induction bone formation,good biocompatibility and bioactivity.%采用电泳沉积法制备了镁合金基羟基磷灰石表面涂层生物复合材料。利用动物体内埋植实验和SBF浸泡实验分别对复合材料生物相容性及生物活性进行研究。将SD大鼠分为对照组和植入组,植入一定的时间后,取植入物周围组织进行切片、HE染色和组织分析;将复合材料恒温浸泡于SBF溶液四周后取出,通过检测复合材料表面生长磷灰石的能力作为评价该材料的生物活性。实验结果是植入物周围出现组织增生,有结缔组织及新生血管的形成,未见明显的组织炎症反应,无多核的巨细胞、淋巴细胞和中性

  4. FABRICATION AND BIOCOMPATIBILITY OF CELL OUTER MEMBRANE MIMETIC SURFACES

    Institute of Scientific and Technical Information of China (English)

    Ming-ming Zong; Yong-kuan Gong

    2011-01-01

    The surface design used for improving biocompatibility is one of the most important issues for the fabrication of medical devices. For mimicking the ideal surface structure of cell outer membrane, a large number of polymers bearing phosphorylcholine (PC) groups have been employed to modify the surfaces of biomaterials and medical devices. It has been demonstrated that the biocompatibility of the modified materials whose surface is required to interact with a living organism has been obviously improved by introducing PC groups. In this review, the fabrication strategies of cell outer membrane mimetic surfaces and their resulted biocompatibilities were summarized.

  5. Cellular biocompatibility of various electrospun nPCL/HA scaffold materials%不同配比纳米羟基磷灰石/聚己内酯复合材料细胞相容性的研究

    Institute of Scientific and Technical Information of China (English)

    李家锋; 万美蓉; 管海虹; 贺文鹏; 张红闯; 张扬; 陈丽娟

    2011-01-01

    目的:观察不同配比nPCL/HA电纺纤维取向薄膜材料的细胞相容性.方法:将人骨髓间充质干细胞(hBMSCs)体外诱导培养为成骨细胞;并经传代培养第5代的人骨髓间充质干细胞,以2×105cm2的密度与不同配比nPCL/HA电纺纤维取向薄膜支架在培养板内共培养,同时以nPCL电纺纤维非取向薄膜材料作为对照,初步观察hBMSCs在不同配比nPCL/HA支架材料上复合培养,对其细胞相容性进行评价.结果:hBMSCs与3种电纺薄膜支架材料均有细胞相容性,细胞能在不同材料表面黏附生长、分化增殖.但是PCL/HA的配比为20∶1电纺纤维取向薄膜材料黏附率(35.3±2.6)%,为3中材料中黏附率最高的一种,材料表面细胞生长良好,体积变大,有伪足生长.结论:PCL/HA的配比为20∶1电纺纤维取向薄膜材料,较适合作为支架材料应用于hBMSCs 为种子细胞的组织工程构建.%Objective To investigate cellular biocompatibility of different nPCL/HA scaffold materials. Methods Electrostatic spinning or electrospinning is an interesting method for producing nonwoven fibers with diameters of submicrometers down to nanometers. Nanofibrous membranes were used in many biomedical applications including drug delivery, wound healing and scaffolding for tissue engineering. Novel bone-scaffolding materials were successfully fabricated by electrospinning from polycaprolactone (PCL) solutions containing nanoparticles of hydroxyapatite (HA). In intro cultured hBMSCs (5th generation) were seeded at the density of 2×105 cell/cm2 onto scaffolds of nPCL/HA and nPCL as control. The cell-material complex was observed in order to evaluate the cellular biocompatibility between cells and materials. Results HBMSCs were shown good adhesion to all 3 types of scaffolds after seeding. The cellular biocompatibility of nPCL/HA (20:1) (35.3±2.6)% was higher than the others. Conclusion Nano-PCL/HA (20:1) was shown significantly higher adhesion rate to h

  6. Infrared study of biocompatible magnetic nanoparticles

    International Nuclear Information System (INIS)

    Magnetic fluids mainly consist of nano sized iron oxide particles (FesO4) that are suspended in carrier liquid. In recent years, substantial progress has been made in developing technologies in the field of magnetic nanoparticles. Techniques based on using of these biocompatible magnetizable complex systems have found application in numerous biological fields. Generally, the magnetic component of the particle is coated by a biocompatible polymer such as PEG (polyethylene glycol), policyholder's as dextran, starch, have been developed. The coating acts to shield the magnetic particle from the surrounding environment. This review is focused on describing the infrared spectroscopy as method that allows checking starting materials and reactions while work is in progress. FTIR method is correlated with others spectral methods as XRD, TEM. Keywords: Magnetic nanoparticles, infrared spectroscopy, biomedicine

  7. A biocompatible magnetic film: synthesis and characterization

    Science.gov (United States)

    Chatterjee, Jhunu; Haik, Yousef; Chen, Ching Jen

    2004-01-01

    Background Biotechnology applications of magnetic gels include biosensors, targeted drug delivery, artificial muscles and magnetic buckles. These gels are produced by incorporating magnetic materials in the polymer composites. Methods A biocompatible magnetic gel film has been synthesized using polyvinyl alcohol. The magnetic gel was dried to generate a biocompatible magnetic film. Nanosized iron oxide particles (γ-Fe2O3, ~7 nm) have been used to produce the magnetic gel. Results The surface morphology and magnetic properties of the gel films were studied. The iron oxide particles are superparamagnetic and the gel film also showed superparamagnetic behavior. Conclusion Magnetic gel made out of crosslinked magnetic nanoparticles in the polymer network was found to be stable and possess the magnetic properties of the nanoparticles. PMID:14761251

  8. Biocompatibility of Coronary Stents

    Directory of Open Access Journals (Sweden)

    Thamarasee M. Jeewandara

    2014-01-01

    Full Text Available Cardiovascular disease is the dominant cause of mortality in developed countries, with coronary artery disease (CAD a predominant contributor. The development of stents to treat CAD was a significant innovation, facilitating effective percutaneous coronary revascularization. Coronary stents have evolved from bare metal compositions, to incorporate advances in pharmacological therapy in what are now known as drug eluting stents (DES. Deployment of a stent overcomes some limitations of balloon angioplasty alone, but provides an acute stimulus for thrombus formation and promotes neointimal hyperplasia. First generation DES effectively reduced in-stent restenosis, but profoundly delay healing and are susceptible to late stent thrombosis, leading to significant clinical complications in the long term. This review characterizes the development of coronary stents, detailing the incremental improvements, which aim to attenuate the major clinical complications of thrombosis and restenosis. Despite these enhancements, coronary stents remain fundamentally incompatible with the vasculature, an issue which has largely gone unaddressed. We highlight the latest modifications and research directions that promise to more holistically design coronary implants that are truly biocompatible.

  9. Doped biocompatible layers prepared by laser

    Science.gov (United States)

    Jelínek, M.; Weiserová, M.; Kocourek, T.; Jurek, K.; Strnad, J.

    2010-03-01

    The contribution deals with KrF laser synthesis and study of doped biocompatible materials with focus on diamond-like carbon (DLC) and hydroxyapatite (HA). Overview of materials used for dopation is given. Experimental results of study of HA layers doped with silver are presented. Films properties were characterized using profilometer, SEM, WDX, XRD and optical transmission. Content of silver in layers moved from 0.06 to 13.7 at %. The antibacterial properties of HA, silver and doped HA layers were studied in vivo using Escherichia coli cells.

  10. Exfoliating biocompatible ferromagnetic Cr-trihalide monolayers.

    Science.gov (United States)

    Liu, Junyi; Sun, Qiang; Kawazoe, Yoshiyuki; Jena, Puru

    2016-03-23

    In addition to spintronics another motivation for exploring ferromagnetic two-dimensional materials is for biomedical applications such as magnetic labeling and hyperthermia treatment of tumors. Unfortunately, the widely studied Mn-containing monolayer is not biocompatible, although it is ferromagnetic. Here using first principles calculations combined with Monte Carlo simulations based on the Ising model, we systematically study a class of 2D ferromagnetic monolayers CrX3 (X = Cl, Br, I). The feasibility of exfoliation from their layered bulk phase is confirmed by the small cleavage energy and high in-plane stiffness. Spin-polarized calculations, combined with self-consistently determined Hubbard U that accounts for strong correlation energy, demonstrate that CrX3 (X = Cl, Br, I) monolayers are ferromagnetic and that Cr is trivalent and carries a magnetic moment of 3 μB; the resulting Cr(3+) ions are biocompatible. The corresponding Curie temperatures for CrCl3, CrBr3 and CrI3 are found to be 66, 86, and 107 K, respectively, which can be increased to 323, 314, and 293 K by hole doping. The biocompatibility and ferromagnetism render these Cr-containing trihalide monolayers unique for applications. PMID:26452203

  11. Synthetic cornea: biocompatibility and optics

    Science.gov (United States)

    Parel, Jean-Marie A.; Kaminski, Stefan; Fernandez, Viviana; Alfonso, E.; Lamar, Peggy; Lacombe, Emmanuel; Duchesne, Bernard; Dubovy, Sander; Manns, Fabrice; Rol, Pascal O.

    2002-06-01

    Purpose. Experimentally find a method to provide a safe surgical technique and an inexpensive and long lasting mesoplant for the restoration of vision in patients with bilateral corneal blindness due to ocular surface and stromal diseases. Methods. Identify the least invasive and the safest surgical technique for synthetic cornea implantation. Identify the most compatible biomaterials and the optimal shape a synthetic cornea must have to last a long time when implanted in vivo. Results. Penetrating procedures were deemed too invasive, time consuming, difficult and prone to long term complications. Therefore a non-penetrating delamination technique with central trephination was developed to preserve the integrity of Descemet's membrane and the anterior segment. Even though this approach limits the number of indications, it is acceptable since the majority of patients only have opacities in the stroma. The prosthesis was designed to fit in the removed tissue plane with its skirt fitted under the delaminated stroma. To improve retention, the trephination wall was made conical with the smallest opening on the anterior surface and a hat-shaped mesoplant was made to fit. The skirt was perforated in its perimeter to allow passage of nutrients and tissues ingrowths. To simplify the fabrication procedure, the haptic and optic were made of the same polymer. The intrastromal biocompatibility of several hydrogels was found superior to current clinically used PMMA and PTFE materials. Monobloc mesoplants made of 4 different materials were implanted in rabbits and followed weekly until extrusion occurred. Some remained optically clear allowing for fundus photography. Conclusions. Hydrogel synthetic corneas can be made to survive for periods longer than 1 year. ArF excimer laser photoablation studies are needed to determine the refractive correction potential of these mesoplants. A pilot FDA clinical trial is needed to assess the mesoplant efficacy and very long-term stability.

  12. In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

    OpenAIRE

    Da Silva G.R.; Lima T.H.; Oréfice R.L.; Fernandes-Cunha G.M.; Silva-Cunha A.; Zhao M; Behar-Cohen F.

    2015-01-01

    Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells ...

  13. In vivo biocompatibility of nanostructured Chitosan/Peo membranes

    Directory of Open Access Journals (Sweden)

    V.A.S. Vulcani

    2015-08-01

    Full Text Available Electrospinning is a technique that allows the preparation of nanofibers from various materials. Chitosan is a natural and abundant easily obtained polymer, which, in addition to those features, proved to be biocompatible. This work used nanostructured chitosan and polyoxyethylene membranes as subcutaneous implants in Wistar rats to evaluate the biocompatibility of the material. Samples of the material and tissues adjacent to the implant were collected 7, 15, 30, 45 and 60 days post-implantation. Macroscopic integration of the material to the tissues was observed in the samples and slides for histopathological examination that were prepared. It was noticed that the material does not stimulate the formation of adherences to the surrounding tissues and that there is initial predominance of neutrophilia and lymphocytosis, with a declining trend according to the increase of time, featuring a non-persistent acute inflammatory process. However, the material showed fast degradation, impairing the macroscopic observation after fifteen days of implantation. It was concluded that the material is biocompatible and that new studies should be conducted, modifying the time of degradation by changes in obtaining methods and verifying the biocompatibility in specific tissues for biomedical applications.

  14. The surface alloying effect of silicon in a binary NiTi-base alloy on the corrosion resistance and biocompatibility of the material

    Science.gov (United States)

    Psakhie, S. G.; Lotkov, A. I.; Meisner, L. L.; Meisner, S. N.; Matveeva, V. A.

    2013-02-01

    The corrosion resistance behavior and cytotoxicity of binary NiTi-base alloy specimens subjected to surface modification by silicon ion beams and the proliferative ability of mesenchymal stem cells of rat marrow on an ion-implanted surface of the alloy have been studied. The silicon ion beam processing of specimen surfaces is shown to bring about a nearly two-fold improvement in the corrosion resistance of the material to attack by aqueous solutions of NaCl (artificial body fluid) and human plasma and a drastic decrease in the nickel concentration after immersion of the specimens into the solutions for ˜3400 and ˜6000 h, respectively (for the artificial plasma solution, a nearly 20-fold decrease in the Ni concentration is observed.)

  15. Biocompatibility of polymethylmethacrylate resins used in dentistry.

    Science.gov (United States)

    Gautam, Rupali; Singh, Raghuwar D; Sharma, Vinod P; Siddhartha, Ramashanker; Chand, Pooran; Kumar, Rakesh

    2012-07-01

    Biocompatibility or tissue compatibility describes the ability of a material to perform with an appropriate host response when applied as intended. Poly-methylmethacrylate (PMMA) based resins are most widely used resins in dentistry, especially in fabrication of dentures and orthodontic appliances. They are considered cytotoxic on account of leaching of various potential toxic substances, most common being residual monomer. Various in vitro and in vivo experiments and cell based studies conducted on acrylic based resins or their leached components have shown them to have cytotoxic effects. They can cause mucosal irritation and tissue sensitization. These studies are not only important to evaluate the long term clinical effect of these materials, but also help in further development of alternate resins. This article reviews information from scientific full articles, reviews, or abstracts published in dental literature, associated with biocompatibility of PMMA resins and it is leached out components. Published materials were searched in dental literature using general and specialist databases, like the PubMED database. PMID:22454327

  16. Adhesion of biocompatible and biodegradable micropatterned surfaces

    NARCIS (Netherlands)

    Kaiser, J.S.; Kamperman, M.M.G.; Souza, E.J.; Schick, B.; Arzt, E.

    2011-01-01

    We studied the effects of pillar dimensions and stiffness of biocompatible and biodegradable micropatterned surfaces on adhesion on different compliant substrates. The micropatterned adhesives were based on biocompatible polydimethylsiloxane (PDMS) and biodegradable poly(lactic-co-glycolic) acid (PL

  17. 骨盆并髋臼骨折内固定材料的生物相容性%Materials biocompatibility in the internal fixation of pelvic fracture combined with acetabular fracture

    Institute of Scientific and Technical Information of China (English)

    高明杰; 陶杰; 周孜辉; 杜琳

    2015-01-01

    背景:骨盆骨折合并髋臼骨折多由高能量暴力伤所致,常伴严重并发症,往往需要外科内固定治疗。目的:探讨骨盆骨折合并髋臼骨折内固定治疗以及材料的生物相容性。方法:以“骨盆骨折,髋臼骨折,内固定,材料;pelvis fracture,acetabulum fracture,internal fixators, materials”为关键词,应用计算机检索2005至2014年万方数据库、清华知网数据库和PubMed数据库,选取有关骨盆骨折合并髋臼骨折内固定治疗以及材料生物相容性研究的文献,同一领域文献选择权威杂志或发表时间为近期的文章,依据纳入排除标准选取29篇文献进行归纳分析。结果与结论:对于骨盆骨折合并髋臼骨折临床多采用重建钢板或拉力螺钉内固定,常用于内固定的重建钢板材料是钛板,弹性模量接近骨的弹性模量,生物相容性好,可在置入前预先弯曲塑形,使之与骨折部位骨面相匹配。可吸收螺钉具有良好的组织相容性,无毒副反应,避免金属螺钉的电解与腐蚀,在内固定早期保持一定的强度,随着时间的推移骨折逐渐愈合,材料强度也缓慢降低,材料最终降解为水和二氧化碳,治疗效果满意。以三维重建技术定制钢板固定和微创内固定是目前骨折治疗的重大进展,临床应用前景广阔。%BACKGROUND:Pelvic fractures combined with acetabular fractures are mostly caused by high-energy violence, often accompanied by severe complications and high mortality, and surgical fixation is preferred in most cases. OBJECTIVE:To investigate the internal fixation of pelvic fracture combined with acetabular fracture and to analyze the material biocompatibility. METHODS:A computer-based search of Wanfang, CNKI and PubMed databases was performed for articles related to the internal fixation of pelvic fracture combined with acetabular fracture and material biocompatibility published

  18. Biocompatibility of Bacterial Cellulose Based Biomaterials

    Directory of Open Access Journals (Sweden)

    Omar P. Troncoso

    2012-12-01

    Full Text Available Some bacteria can synthesize cellulose when they are cultivated under adequate conditions. These bacteria produce a mat of cellulose on the top of the culture medium, which is formed by a three-dimensional coherent network of pure cellulose nanofibers. Bacterial cellulose (BC has been widely used in different fields, such as the paper industry, electronics and tissue engineering due to its remarkable mechanical properties, conformability and porosity. Nanocomposites based on BC have received much attention, because of the possibility of combining the good properties of BC with other materials for specific applications. BC nanocomposites can be processed either in a static or an agitated medium. The fabrication of BC nanocomposites in static media can be carried out while keeping the original mat structure obtained after the synthesis to form the final nanocomposite or by altering the culture media with other components. The present article reviews the issue of biocompatibility of BC and BC nanocomposites. Biomedical aspects, such as surface modification for improving cell adhesion, in vitro and in vivo studies are given along with details concerning the physics of network formation and the changes that occur in the cellulose networks due to the presence of a second phase. The relevance of biocompatibility studies for the development of BC-based materials in bone, skin and cardiovascular tissue engineering is also discussed.

  19. Biocompatibility of dental alloys

    Energy Technology Data Exchange (ETDEWEB)

    Braemer, W. [Heraeus Kulzer GmbH and Co. KG, Hanau (Germany)

    2001-10-01

    Modern dental alloys have been used for 50 years to produce prosthetic dental restorations. Generally, the crowns and frames of a prosthesis are prepared in dental alloys, and then veneered by feldspar ceramics or composites. In use, the alloys are exposed to the corrosive influence of saliva and bacteria. Metallic dental materials can be classified as precious and non-precious alloys. Precious alloys consist of gold, platinum, and small amounts of non-precious components such as copper, tin, or zinc. The non-precious alloys are based on either nickel or cobalt, alloyed with chrome, molybdenum, manganese, etc. Titanium is used as Grade 2 quality for dental purposes. As well as the dental casting alloys, high purity electroplated gold (99.8 wt.-%) is used in dental technology. This review discusses the corrosion behavior of metallic dental materials with saliva in ''in vitro'' tests and the influence of alloy components on bacteria (Lactobacillus casei and Streptococcus mutans). The test results show that alloys with high gold content, cobalt-based alloys, titanium, and electroplated gold are suitable for use as dental materials. (orig.)

  20. Preparation of a biocompatible magnetic film from an aqueous ferrofluid

    International Nuclear Information System (INIS)

    Very promising nanoparticles for biomedical applications or in medical drug targeting are superparamagnetic nanoparticles based on a core consisting of iron oxides (SPION) that can be targeted through external magnets. Polyvinyl alcohol (PVA) is a unique synthetic biocompatible polymer that can be chemically cross-linked to form a gel. Biotechnology applications of magnetic gels include biosensors, targeted drug delivery, artificial muscles and magnetic buckles. These gels are produced by incorporating magnetic materials in the polymer composites. In this paper we report the synthesis of an aqueous ferrofluid and the preparation of a biocompatible magnetic gel with polyvinyl alcohol and glutharaldehyde (GTA). HClO4 was used to induce the peptization since this kind of ferrofluid does not have surfactant. The magnetic gel was dried to generate a biocompatible film

  1. Preparation of a biocompatible magnetic film from an aqueous ferrofluid

    Science.gov (United States)

    Albornoz, Cecilia; Jacobo, Silvia E.

    2006-10-01

    Very promising nanoparticles for biomedical applications or in medical drug targeting are superparamagnetic nanoparticles based on a core consisting of iron oxides (SPION) that can be targeted through external magnets. Polyvinyl alcohol (PVA) is a unique synthetic biocompatible polymer that can be chemically cross-linked to form a gel. Biotechnology applications of magnetic gels include biosensors, targeted drug delivery, artificial muscles and magnetic buckles. These gels are produced by incorporating magnetic materials in the polymer composites. In this paper we report the synthesis of an aqueous ferrofluid and the preparation of a biocompatible magnetic gel with polyvinyl alcohol and glutharaldehyde (GTA). HClO 4 was used to induce the peptization since this kind of ferrofluid does not have surfactant. The magnetic gel was dried to generate a biocompatible film.

  2. BIOCOMPATIBILITY EVALUATION OF XANTHAN/CHONDROITIN SULFATE HYDROGELS

    Directory of Open Access Journals (Sweden)

    Ana-Maria Oprea

    2012-03-01

    Full Text Available The in vitro and in vivo biocompatibility of xanthan/chondroitin sulfate hydrogels (X/CS in differentmixing ratios was investigated. The in vitro biocompatibility evaluation was performed by a chemiluminescent assayusing microorganisms such as Saccharomyces pombe. The cellular growth of S. pombe in presence of thexanthan/chondroitin sulfate hydrogels containing up to 20 % chondroitin sulfate was examinated comparatively withxanthan hydrogel.The in vivo evaluation was performed by toxicity test and subcutaneously implantation in rats. It has been establisheda lethal dose (LD50 bigger than 3200 mg/kg for all studied hydrogels, therefore they are nontoxic materials.The in vivo 30 days testing performed by subcutaneous implantation showed that the X/CS matrices were easilyabsorbed without side-effects, demonstrating their biocompatibility and effectiveness as potential drug delivery systems.

  3. Biocompatibility of Ti-alloys for long-term implantation.

    Science.gov (United States)

    Abdel-Hady Gepreel, Mohamed; Niinomi, Mitsuo

    2013-04-01

    The design of new low-cost Ti-alloys with high biocompatibility for implant applications, using ubiquitous alloying elements in order to establish the strategic method for suppressing utilization of rare metals, is a challenge. To meet the demands of longer human life and implantation in younger patients, the development of novel metallic alloys for biomedical applications is aiming at providing structural materials with excellent chemical, mechanical and biological biocompatibility. It is, therefore, likely that the next generation of structural materials for replacing hard human tissue would be of those Ti-alloys that do not contain any of the cytotoxic elements, elements suspected of causing neurological disorders or elements that have allergic effect. Among the other mechanical properties, the low Young's modulus alloys have been given a special attention recently, in order to avoid the occurrence of stress shielding after implantation. Therefore, many Ti-alloys were developed consisting of biocompatible elements such as Ti, Zr, Nb, Mo, and Ta, and showed excellent mechanical properties including low Young's modulus. However, a recent attention was directed towards the development of low cost-alloys that have a minimum amount of the high melting point and high cost rare-earth elements such as Ta, Nb, Mo, and W. This comes with substituting these metals with the common low cost, low melting point and biocompatible metals such as Fe, Mn, Sn, and Si, while keeping excellent mechanical properties without deterioration. Therefore, the investigation of mechanical and biological biocompatibility of those low-cost Ti-alloys is highly recommended now lead towards commercial alloys with excellent biocompatibility for long-term implantation. PMID:23507261

  4. Biocompatible and Bioeliminable Hydrophilic Polymers

    Institute of Scientific and Technical Information of China (English)

    Paolo; FerrutiUniversità

    2007-01-01

    1 Introduction This presentation will report on some recent results obtained in Milan on two polymer families of biomedical interest, namely poly(N-vinyl-2-pyrrolidinone) and polyamidoamines. 2 Results and DiscussionPoly(N-vinyl-2-pyrrolidinone) (PVP) is a well known bioactive and biocompatible polymer. In its soluble form, it is largely used as excipient of oral pharmaceutical formulations, especially for its high water solubilising power.In its crosslinked form, it plays a relevant role as biomateria...

  5. 磷酸钙骨水泥/骨形态发生蛋白复合人工骨的生物相容性%Biocompatibility of calcium phosphate cement/BMP composite as bone graft material

    Institute of Scientific and Technical Information of China (English)

    孙明林; 胡蕴玉; 贾新斌; 李丹; 刘忠湘; 朱德生

    2001-01-01

    AIM To construct CPC/BMP composite by combining CPC with BMP, and to detect their biocompatibity. METHODS The toxicity, pyrogen, hemolytic activities and immunogen were detected by means of animal test, cell culture, hemolysis test and histochemical technique. RESULTS The composites were found no toxicity and no pyrogen by animal test, no hemolytic activities and couldn't influence the coagulate of blood in vitro. When the materials were grafted into the muscle pounches in the thigh of mice or rabbits, no obvious specific antibodies produced were detected in serum by ELISA, nor were significant immune reaction of foreign body observed by histochemistry technique. The composites were also testified no cytotoxicity in vitor for the proliferation of cultured cells couldn' be inhibited. CONCLUSION The CPC/BMP composite might have good biocompatibility and be safe for clinical use.%目的将自行合成的磷酸钙骨水泥(CPC)作为载体与BMP复合成人工骨,检测其生物相容性. 方法制备CPC/BMP及CPC骨块,通过体外实验、细胞培养、动物实验等方法观察其毒性、免疫原性、对血液系统的影响等生物相容性指标. 结果动物实验表明材料属无毒级,不含致热原,体外试验不引起溶血反应,对凝血功能无明显影响. 植入兔或小鼠肌袋内未检测出特异性抗体. 组织学检查未见免疫排斥反应,对肌肉无刺激作用.对体外培养的细胞增殖没有明显抑制作用.结论材料有较好的生物相容性,临床使用安全.

  6. The quantification of biocompatibility: toward a new definition

    Science.gov (United States)

    Ratner, Buddy

    2008-03-01

    Implantable medical devices, and the biomaterials that comprise them, form a 100B business worldwide. Medical devices save lives and/or improve the quality of life for millions. Tissue engineering also makes extensive use of biomaterials -- biomaterials are an enabling technology for tissue engineering. A central word to understanding the effectiveness of such materials and devices is biocompatibility. The word ``biocompatible'' is widely used in reference to biomaterials and medical devices and most everyone has some value understanding of its meaning. Many formal definitions have been proposed for this word, but it is still largely used in an imprecise manner. Four descriptions or definitions of biocompatibility will be reviewed: a widely adopted definition from a consensus conference, a surgeon's perspective on this word, the regulatory agency view and the factors that clearly influence biocompatibility. In this talk, the classical definition of biocompatibility will be contrasted to a newer definition embracing molecular concepts and the understanding of normal wound healing. The biological data on the in vivo healing responses of mammals to implants will be described. A strategy to improve the healing of biomaterials will be presented. It is based upon surface molecular engineering. First, non-specific protein adsorption must be inhibited. Strategies to achieve this design parameter will be presented. Then methods to deliver the specific protein signals will be addressed. Matricellular proteins such as osteopontin, thrombospondin 2 and SPARC will be introduced with an emphasis on exploiting the special reactivity of such proteins. A discussion of the influence of surface textures and porosities will also be presented. Finally a new scheme based upon macrophage phenotypic pathways will be proposed that may allow a quantitative measure of extent of biocompatibility.

  7. Nanocellulose for Biomedical Applications : Modification, Characterisation and Biocompatibility Studies

    OpenAIRE

    Hua, Kai

    2015-01-01

    In the past decade there has been increasing interest in exploring the use of nanocellulose in medicine. However, the influence of the physicochemical properties of nanocellulose on the material´s biocompatibility has not been fully investigated.  In this thesis, thin films of nanocellulose from wood (NFC) and from Cladophora algae (CC) were modified by the addition of charged groups on their surfaces and the influence of these modifications on the material´s physicochemical properties and on...

  8. Biocompatibility of polytetrafluoroethylene combined with type I collagen as a nose filler material%聚四氟乙烯联合Ⅰ型胶原作为隆鼻高分子材料的生物相容性

    Institute of Scientific and Technical Information of China (English)

    尹中普; 孙晓

    2015-01-01

    BACKGROUND:Polytetrafluoroethylene (PTEE) as a nose filer material has advantages on the resistant to corrosion, chemical stability and so on. However, its linear expansion coefficient is large easily leading to infection and rejection, and its application has some limitations. OBJECTIVE:To compare the cel toxicity, inflammatory infiltrates and biocompatibility indexesin vivo between PTEE and PTEE combined with type Ⅰ colagen. METHODS: MTT method was used to detect the relative proliferation rate of L929 cels cultured with PTEE extract or PTEE combined with type Ⅰ colagen extract; an electron microscope was used to observe the cel growth. PTEE or PTEE combined with type Ⅰ colagen was embedded under the nasal dorsal fascia of New Zealand white rabbits for 7 days, and hematoxylin-eosin staining was used to detect whether nasal mucosa epithelial tissue had inflammatory infiltration. Systemic toxicity, alergies, pyrogen reaction and deaths of rabbits were observed after injection of two kinds of material extracts by ear vein. RESULTS AND CONCLUSION: The cytotoxicity and inflammatory infiltration were milder in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). The alergic reactions and pyrogen reactions were fewer in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). These findings indicate that PTEE combined with type Ⅰ colagen as the nose filer material has better biocompatibility.%背景:高分子材料聚四氟乙烯膨体作为隆鼻填充材料具有耐腐蚀、化学性质稳定等优点,但其线膨胀系数较大,易引发感染及排异反应,故应用有一定局限性.目的:对比聚四氟乙烯和聚四氟乙烯联合Ⅰ型胶原作为隆鼻填充材料的细胞毒性、埋植后的炎性浸润及体内生物相容性.方法:采用MTT法检测聚四氟乙烯浸提液和聚四氟乙烯联合Ⅰ型胶原浸提液培养L929细胞的细胞增殖.采用电子显微镜观察聚四氟乙烯浸提液和聚四氟乙

  9. Biostability and biocompatibility of modified polyurethane elastomers

    Science.gov (United States)

    Christenson, Elizabeth Marie

    Several strategies have been employed to increase the biostability of medical grade polyurethanes while maintaining the desirable properties of current poly(ether urethanes). It was hypothesized that polyurethane surface chemistry controls biodegradation/biostability that can lead to ultimate failure/success of these materials in clinical applications. Chemical modification or replacement of the susceptible soft segment was evaluated as a design strategy to increase the biostability of medical grade polyurethanes. The effect of soft segment chemistry on the phase morphology, mechanical properties and in vivo response of commercial polyurethanes were compared. Poly(ether urethane) (PEU), silicone-modified poly(ether urethane) (PEU-S), poly(carbonate urethane) (PCU) and silicone-modified poly(carbonate urethane) (PCU-S) elastomers were investigated. AFM phase imaging indicated that the overall two-phase morphology of poly(ether urethanes), necessary for its thermoplastic elastomeric properties, was not disrupted by changing the soft segment chemistry. All of the polyurethanes exhibited thermoplastic elastomeric behavior similar to that of the poly(ether urethane). Following material characterization, the biocompatibility of the polyurethane elastomers was evaluated using a subcutaneous cage implant protocol. All of the polyurethanes tested retained the excellent biocompatibility typical of poly(ether urethane) elastomers. Overall, the candidate polyurethanes were concluded to be suitable replacements of current poly(ether urethane) elastomers in medical applications. The results from the cage implant study and cell culture experiments indicated that monocytes adhere, differentiate and fuse to form foreign body giant cells (FBGCs) on all of the polyurethane specimens. It is now generally accepted that the reactive oxygen species released by these adherent macrophages and FBGCs initiate PEU biodegradation. ATR-FTIR analysis of explanted samples provided evidence of

  10. Biocompatibility of Compounds of Extracellular Matrix and Thermally Reversible Hydrogel

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A new scaffold material composed of extracellular matrix (ECM) and thermal sensitive hydrogel (HG), and evaluated its biocompatibility were investigated. We cultured bladder smooth muscle cells with this compound material, and then observed with phase contrast microscopy and scanning electron microscope (SEM) to assess the cell growth and morphology. The cell adhesion and proliferation were detected with MTT assay and cell count. Results show the ECM/HG compounds appeared as a net-like and red-stained construction with enough meshes and without any cellular fragments. 6 h after implantation, cells were observed adhere on the compounds and extend spurious along the fibers 12 h later. Under SEM even some ECM was observed to be secreted. MTT assay shows there was obvious statistic difference among 3 groups (P<0.05).ECM/HG compound materials show a good biocompatibility, which confirms that it would be an ideal tissue engineering scaffolds.

  11. Radiation crosslinking of biocompatible polymers

    Czech Academy of Sciences Publication Activity Database

    Sedláček, Ondřej; Kučka, Jan; Monnery, B. D.; Hoogenboom, R.; Hrubý, Martin

    Bratislava : Young Scientists Council of Polymer Institute of Slovak Academy of Sciences, 2016. s. 89. ISBN 978-80-970923-8-2. [Bratislava Young Polymer Scientists workshop /6./ - BYPoS 2016. 14.03.2016-18.03.2016, Ždiar] R&D Projects: GA MŠk(CZ) LO1507; GA MŠk(CZ) LH14079; GA MZd(CZ) NV15-25781A; GA ČR(CZ) GA13-08336S Institutional support: RVO:61389013 Keywords : radiolysis * radiotherapeutics * biocompatible polymer s Subject RIV: CD - Macromolecular Chemistry

  12. Evaluation of the biocompatibility of a new biomembrane

    Directory of Open Access Journals (Sweden)

    Fatima Mrue

    2004-06-01

    Full Text Available Biocompatibility has been considered one of the most important items to validate a biomaterial for its application in human organisms. The present work evaluates the biocompatibility of a new biomembrane using in vivo assay in different animal species. The experiments to evaluate the cellular reaction were carried out through the implantation of the material into the subcutaneous tissue of animals and the results showed a good reaction of the host tissue without any signal of fibrosis or rejection. The cell adhesion experiments were done by means of the measure of the DNA content on the material surface after its implantation into the subcutaneous tissue of animals and the results showed a growing number of DNA that was proportional to the time of implantation. The healing process was evaluated using a dermal ulcer model and the results showed a good tissue repair resembling a physiologic process. The overall results presented here lead to the conclusion that this new biomembrane is a biocompatible material but more research must be done, as it is a new material desired for medical use.

  13. Forsterite Nanopowder: Structural Characterization and Biocompatibility Evaluation

    Institute of Scientific and Technical Information of China (English)

    M.A.Naghiu; M.Gorea; E.Mutch; F.Kristaly; M.Tomoaia-Cotisel

    2013-01-01

    Forsterite,a new biocompatible material was synthesized from Mg(NO3)2·6H20 and TEOS by using the sol-gel method.The material was then heated at 800,900 and 1000 ℃.The forsterite was noticed as the main crystalline phase in the material fired at 900 and 1000 ℃,while periclase (MgO) was present in all the samples.The tests confirm that in the first two samples forsterite is present as crystallites <60 nm,while in the sample synthesized at 1000 ℃ it forms aggregates of micrometre-sized grains.In vitro test was performed by immersing the forsterite powder in the simulating body fluids (SBF) and hydroxyapatite formation on the surface was investigated.We could evidence the formation of hydroxyapatite on the forsterite surface after 7 days of immersion.The MTT test confirmed that forsterite powders dissolution promote osteoblast proliferation of the human-type osteoblasts with no significant cytotoxicity effects.

  14. Biocompatibility of Chitosan Carriers with Application in Drug Delivery

    OpenAIRE

    Ana Grenha; Susana Rodrigues; Carmen Remuñán López; Marita Dionísio

    2012-01-01

    Chitosan is one of the most used polysaccharides in the design of drug delivery strategies for administration of either biomacromolecules or low molecular weight drugs. For these purposes, it is frequently used as matrix forming material in both nano and micron-sized particles. In addition to its interesting physicochemical and biopharmaceutical properties, which include high mucoadhesion and a great capacity to produce drug delivery systems, ensuring the biocompatibility of the drug delivery...

  15. Reinforcement of bacterial cellulose aerogels with biocompatible polymers

    OpenAIRE

    Pircher, N.; Veigel, S.; Aigner, N; Nedelec, J. M.; Rosenau, T.; Liebner, F.

    2014-01-01

    Bacterial cellulose (BC) aerogels, which are fragile, ultra-lightweight, open-porous and transversally isotropic materials, have been reinforced with the biocompatible polymers polylactic acid (PLA), polycaprolactone (PCL), cellulose acetate (CA), and poly(methyl methacrylate) (PMMA), respectively, at varying BC/polymer ratios. Supercritical carbon dioxide anti-solvent precipitation and simultaneous extraction of the anti-solvent using scCO2 have been used as core techniques for incorporating...

  16. Polycrystalline Silicon: a Biocompatibility Assay

    International Nuclear Information System (INIS)

    Polycrystalline silicon (poly-Si) layers were functionalized through the growth of biomimetic hydroxyapatite (HA) on their surface. HA is the mineral component of bones and teeth and thus possesses excellent bioactivity and biocompatibility. MG-63 osteoblast-like cells were cultured on both HA-coated and un-coated poly-Si surfaces for 1, 3, 5 and 7 days and toxicity, proliferation and cell morphology were investigated. The results revealed that the poly-Si layers were bioactive and compatible with the osteoblast-like cells. Nevertheless, the HA coating improved the cell interactions with the poly-Si surfaces based on the cell affinity to the specific chemical composition of the bone-like HA and/or to the higher HA roughness.

  17. Z nanooblaganjem do izboljšanja biokompatibilnosti vsadkov: Improving biocompatibility of implants with nanolayering:

    OpenAIRE

    Dolenc, Andrej; GAŠPERLIN, MIRJANA; Homar, Miha; Kristl, Julijana

    2006-01-01

    Biocompatibility of different materials in the body is very complicated phenomenon. Although structural characteristics of implant are decisive for its specific purpose, biologic response mainly depends on surface characteristics of the implant and its interaction with biologic environment. Nanolayering of the implant to improve biocompatibility presents a recent innovation in this field of research. One method of nanolayering is layer-by-layer assembly which has many advantages over other na...

  18. In vivo qualitative analysis of the biocompatibility of different cyanoacrylate-based adhesives

    OpenAIRE

    Rafael Tobias Moretti Neto; Isabel Mello; Ana Beatriz da Silveira Moretti; Carlos Roberto Colombo Robazza; Alessandro Antônio Costa Pereira

    2008-01-01

    Cyanocrylates have been widely used in the medical and dental fields for several years. In Dentistry, cyanoacrylates have been used for suturing, pulp capping, as retrofilling material in endodontic surgeries, and as cervical plug for pulpless teeth bleaching. The biocompatibility of these adhesives has been the topic of many researches and subcutaneous implantation is an effective methodology for these studies. The present study evaluated the biocompatibility of three different cyanoacrylate...

  19. Boron-doped nanocrystalline diamond electrodes for neural interfaces: In vivo biocompatibility evaluation

    OpenAIRE

    María eAlcaide; Andrew eTaylor; Morten eFjorback; Vladimir eZachar; Cristian Pablo Pennisi

    2016-01-01

    Boron-doped nanocrystalline diamond (BDD) electrodes have recently attracted attention as materials for neural electrodes due to their superior physical and electrochemical properties, however their biocompatibility remains largely unexplored. In this work, we aim to investigate the in vivo biocompatibility of BDD electrodes in relation to conventional titanium nitride (TiN) electrodes using a rat subcutaneous implantation model. High quality BDD films were synthesized on electrodes intended ...

  20. Boron-Doped Nanocrystalline Diamond Electrodes for Neural Interfaces: In vivo Biocompatibility Evaluation

    OpenAIRE

    Alcaide, María; Taylor, Andrew; Fjorback, Morten; Zachar, Vladimir; Pennisi, Cristian P.

    2016-01-01

    Boron-doped nanocrystalline diamond (BDD) electrodes have recently attracted attention as materials for neural electrodes due to their superior physical and electrochemical properties, however their biocompatibility remains largely unexplored. In this work, we aim to investigate the in vivo biocompatibility of BDD electrodes in relation to conventional titanium nitride (TiN) electrodes using a rat subcutaneous implantation model. High quality BDD films were synthesized on electrodes intended ...

  1. Frontiers in biomaterials the design, synthetic strategies and biocompatibility of polymer scaffolds for biomedical application

    CERN Document Server

    Cao, Shunsheng

    2014-01-01

    Frontiers in Biomaterials: The Design, Synthetic Strategies and Biocompatibility of Polymer Scaffolds for Biomedical Application, Volume 1" highlights the importance of biomaterials and their interaction with biological system. The need for the development of biomaterials as scaffold for tissue regeneration is driven by the increasing demands for materials that mimic functions of extracellular matrices of body tissues.This ebook covers the latest challenges on the biocompatibility of scaffold overtime after implantation and discusses the requirement of innovative technologies and strategies f

  2. In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues

    OpenAIRE

    Prokić B.B.; Bačić G.; Prokić B.; Kalijadis Ana; Todorović Vera; Puškaš Nela; Vidojević D.; Laušević Mila; Laušević Z.

    2012-01-01

    In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic)...

  3. Tantalum, Niobium and Titanium Coatings for Biocompatibility Improvement of Dental Implants

    OpenAIRE

    Vajihesadat Mortazavi; Mohammad Hossein Fathi

    2007-01-01

    Introduction: Metals have a wide range of applications in implant and prosthetic materials in dentistry.Corrosion resistance and biocompatibility of metals should be improved in order to utilizethem as biomaterials. The aim of this work was to prepare metallic coatings on 316L stainless steel dental implants, to evaluate the corrosion characteristics of the uncoated and metallic coated dentalimplants as an indication of biocompatibility and, to compare the effect of the type of the coatings o...

  4. Biocompatibility of nanostructured boron doped diamond for the attachment and proliferation of human neural stem cells

    Science.gov (United States)

    Taylor, Alice C.; Vagaska, Barbora; Edgington, Robert; Hébert, Clément; Ferretti, Patrizia; Bergonzo, Philippe; Jackman, Richard B.

    2015-12-01

    Objective. We quantitatively investigate the biocompatibility of chemical vapour deposited (CVD) nanocrystalline diamond (NCD) after the inclusion of boron, with and without nanostructuring. The nanostructuring method involves a novel approach of growing NCD over carbon nanotubes (CNTs) that act as a 3D scaffold. This nanostructuring of BNCD leads to a material with increased capacitance, and this along with wide electrochemical window makes BNCD an ideal material for neural interface applications, and thus it is essential that their biocompatibility is investigated. Approach. Biocompatibility was assessed by observing the interaction of human neural stem cells (hNSCs) with a variety of NCD substrates including un-doped ones, and NCD doped with boron, which are both planar, and nanostructured. hNSCs were chosen due to their sensitivity, and various methods including cell population and confluency were used to quantify biocompatibility. Main results. Boron inclusion into NCD film was shown to have no observable effect on hNSC attachment, proliferation and viability. Furthermore, the biocompatibility of nanostructured boron-doped NCD is increased upon nanostructuring, potentially due to the increased surface area. Significance. Diamond is an attractive material for supporting the attachment and development of cells as it can show exceptional biocompatibility. When boron is used as a dopant within diamond it becomes a p-type semiconductor, and at high concentrations the diamond becomes quasi-metallic, offering the prospect of a direct electrical device-cell interfacing system.

  5. Biodegradable/biocompatible coated metal implants for orthopedic applications.

    Science.gov (United States)

    Saleh, Mohamed M; Touny, A H; Al-Omair, Mohammed A; Saleh, M M

    2016-05-12

    Biocompatible metals have been suggested as revolutionary biomaterials for bone-grafting therapies. Although metals and their alloys are widely and successfully used in producing biomedical implants due to their good mechanical properties and corrosion resistance, they have a lack in bioactivity. Therefore coating of the metal surface with calcium phosphates (CaP) is a benign way to achieve well bioactivity and get controlled corrosion properties. The biocompatibility and bioactivity calcium phosphates (CaP) in bone growth were guided them to biomedical treatment of bone defects and fractures. Many techniques have been used for fabrication of CaP coatings on metal substrates such as magnesium and titanium. The present review will focus on the synthesis of CaP and their relative forms using different techniques especially electrochemical techniques. The latter has always been known of its unique way of optimizing the process parameters that led to a control in the structure and characteristics of the produced materials. PMID:27175470

  6. Biocompatibility of chemical-vapour-deposited diamond.

    Science.gov (United States)

    Tang, L; Tsai, C; Gerberich, W W; Kruckeberg, L; Kania, D R

    1995-04-01

    The biocompatibility of chemical-vapour-deposited (CVD) diamond surfaces has been assessed. Our results indicate that CVD diamond is as biocompatible as titanium (Ti) and 316 stainless steel (SS). First, the amount of adsorbed and 'denatured' fibrinogen on CVD diamond was very close to that of Ti and SS. Second, both in vitro and in vivo there appears to be less cellular adhesion and activation on the surface of CVD diamond surfaces compared to Ti and SS. This evident biocompatibility, coupled with the corrosion resistance and notable mechanical integrity of CVD diamond, suggests that diamond-coated surfaces may be highly desirable in a number of biomedical applications. PMID:7654876

  7. Biocompatibility of crystalline opal nanoparticles

    Directory of Open Access Journals (Sweden)

    Hernández-Ortiz Marlen

    2012-10-01

    Full Text Available Abstract Background Silica nanoparticles are being developed as a host of biomedical and biotechnological applications. For this reason, there are more studies about biocompatibility of silica with amorphous and crystalline structure. Except hydrated silica (opal, despite is presents directly and indirectly in humans. Two sizes of crystalline opal nanoparticles were investigated in this work under criteria of toxicology. Methods In particular, cytotoxic and genotoxic effects caused by opal nanoparticles (80 and 120 nm were evaluated in cultured mouse cells via a set of bioassays, methylthiazolyldiphenyl-tetrazolium-bromide (MTT and 5-bromo-2′-deoxyuridine (BrdU. Results 3T3-NIH cells were incubated for 24 and 72 h in contact with nanocrystalline opal particles, not presented significant statistically difference in the results of cytotoxicity. Genotoxicity tests of crystalline opal nanoparticles were performed by the BrdU assay on the same cultured cells for 24 h incubation. The reduction of BrdU-incorporated cells indicates that nanocrystalline opal exposure did not caused unrepairable damage DNA. Conclusions There is no relationship between that particles size and MTT reduction, as well as BrdU incorporation, such that the opal particles did not induce cytotoxic effect and genotoxicity in cultured mouse cells.

  8. Biofunctionalised biocompatible titania coatings for implants

    Energy Technology Data Exchange (ETDEWEB)

    Faust, V.; Heidenau, F.; Stenzel, F. [Bayreuth Univ. (Germany). Friedrich-Baur-Research Inst. for Biomaterials; Schmidgall, J.; Lipps, G. [Bayreuth Univ. (Germany). Biochemistry Dept.; Ziegler, G. [Bayreuth Univ. (Germany). Friedrich-Baur-Research Inst. for Biomaterials; Bayreuth Univ. (Germany). Inst. for Materials Research

    2002-07-01

    The biological response of an organism to an implant can be influenced by structuring and/or functionalisation of the implant surface. The goal of our study is to improve the osseointegration of orthopaedic endoprothesis by coating metal substrates with dense or nanoporous titania layers combined with biofunctionalisation of the surface with peptides and proteins. The sol-gel method is used to produce titania coatings on medical relevant substrates such as titanium or titanium alloys. Control of the educt ratios and the processing, like drying the specimens in air (xerogel) or supercritical drying in an autoclave unit (aerogel), results in dense or nanoporous titania films. Pore diameters can be adjusted between 10 and 120 nanometers. A remarkable characteristic of the coatings is the high number of surface hydroxy functionalities even after calcination. These reactive groups give ideal conditions for the biofunctionalisation. The immobilisation of biological active substances is carried out by successive covalent silanisation with an aminosilane using a dicarbonic acid as a spacer molecule, and binding of peptides to the spacers. Biocompatibility and cytotoxicity of the materials were tested with cell culture assays. (orig.)

  9. Material characteristics and biocompatibility of plastic and metal biliary stents%塑料与金属胆管支架的材料特征及其生物相容性

    Institute of Scientific and Technical Information of China (English)

    赵冬梅; 蒋丹娜; 刘侠

    2011-01-01

    BACKGROUND: Implantation of plastic and metal biliary stent under digestive endoscopy is an effective means in the treatment of benign and malignant bile duct obstruction. Particularly stent implantation provides great clinical application value for high biliary obstruction in biliary cancer patients who are not suitable for surgical treatment and need to eliminate jaundice,accommodate physiologic d rainage, prolong survival, and improve quality of life.OBJECTIVE: To compare the clinical effects of endoscopic implantation of plastic and metal biliary stents in the treatment of benign and malignant bile duct obstruction, and to explore the biocompatibility of plastic and metal biliary stents with the host.METHODS: Using "bile duct obstruction, plastic biliary stent, metal biliary stent, biocompatibility" in Chinese and "cerebrovascular disease, stent, biocompatibility" in English as the key words, China Academic Journal Full-text Database (CNKI:1989/2009) and Medline (1989/2009) database were searched online by the first author. Meta analysis and repeatable studies were excluded. Totally 35 studies were screened and evaluated, focusing on the research progress, complications, and biocompatibility in treatment of plastic and metal biliary stent.RESULTS AND CONCLUSION: Endoscopic biliary stent implantation is the principal means in the treatment of benign and malignant biliary obstruction. Biliary stent includes metal stent and plastic stent. Metal stents have exhibited great advantages in preventing the growth of bacteria and maintaining stent patency, but the price is expensive. Plastic stents are easy for bacterial attachment, biliary sludge deposition causes stent obstruction, but they are easy to replace and low price. The biocompatibility of plastic and metal biliary stents needs to be improved, for patients with malignant biliary obstruction, bile duct stent implantation in combined with radiation therapy will help to prevent stent obstruction. Further

  10. Carbon Fiber Biocompatibility for Implants

    Science.gov (United States)

    Petersen, Richard

    2016-01-01

    Carbon fibers have multiple potential advantages in developing high-strength biomaterials with a density close to bone for better stress transfer and electrical properties that enhance tissue formation. As a breakthrough example in biomaterials, a 1.5 mm diameter bisphenol-epoxy/carbon-fiber-reinforced composite rod was compared for two weeks in a rat tibia model with a similar 1.5 mm diameter titanium-6-4 alloy screw manufactured to retain bone implants. Results showed that carbon-fiber-reinforced composite stimulated osseointegration inside the tibia bone marrow measured as percent bone area (PBA) to a great extent when compared to the titanium-6-4 alloy at statistically significant levels. PBA increased significantly with the carbon-fiber composite over the titanium-6-4 alloy for distances from the implant surfaces of 0.1 mm at 77.7% vs. 19.3% (p enhanced implant osseointegration. Carbon fibers acting as polymer coated electrically conducting micro-biocircuits appear to provide a biocompatible semi-antioxidant property to remove damaging electron free radicals from the surrounding implant surface. Further, carbon fibers by removing excess electrons produced from the cellular mitochondrial electron transport chain during periods of hypoxia perhaps stimulate bone cell recruitment by free-radical chemotactic influences. In addition, well-studied bioorganic cell actin carbon fiber growth would appear to interface in close contact with the carbon-fiber-reinforced composite implant. Resulting subsequent actin carbon fiber/implant carbon fiber contacts then could help in discharging the electron biological overloads through electrochemical gradients to lower negative charges and lower concentration. PMID:26966555

  11. Biocompatible two-layer tantalum/titania-polymer hybrid coating.

    Science.gov (United States)

    Cortecchia, Elisa; Pacilli, Annalisa; Pasquinelli, Gianandrea; Scandola, Mariastella

    2010-09-13

    Using a two-step procedure, radiopaque and biocompatible coatings were obtained, consisting of a tantalum layer deposited by sputtering technique and of an upper organic-inorganic hybrid layer synthesized via sol-gel. As shown by radiographic images, tantalum confers to plastic substrates good X-ray visibility, adjustable via control of deposition time, but its adhesion to the substrate is poor and manipulation easily damages the metal layer. Polymer-titania hybrid coatings, synthesized using poly-ε-caprolactone (PCL) or carboxy-terminated polydimethylsiloxane (PDMS) as organic precursors, were applied on the metal layer as biocompatible protective coatings. Biocompatibility is demonstrated by cytotoxicity tests conducted using vascular wall resident-mesenchymal stem cells (VW-MSCs). Both coatings show very good adhesion to the substrate, showing no sign of detachment upon large substrate deformations. Under such conditions, SEM observations show that the PCL-containing hybrid forms cracks, whereas the PDMS-based hybrid does not crack, suggesting possible applications of the latter material as a protective layer of sputtered tantalum radiopaque markers for flexible medical devices. PMID:20831278

  12. Biocompatibility of two experimental scaffolds for regenerative endodontics

    Science.gov (United States)

    Setzer, Frank C.; Trope, Martin; Karabucak, Bekir

    2016-01-01

    Objectives The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated. Materials and Methods One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 104, 2.5 × 104, and 5.0 × 104. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility). Results Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 104 hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01). Conclusions The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes. PMID:27200277

  13. Biocompatibility of Chitosan Carriers with Application in Drug Delivery

    Directory of Open Access Journals (Sweden)

    Ana Grenha

    2012-09-01

    Full Text Available Chitosan is one of the most used polysaccharides in the design of drug delivery strategies for administration of either biomacromolecules or low molecular weight drugs. For these purposes, it is frequently used as matrix forming material in both nano and micron-sized particles. In addition to its interesting physicochemical and biopharmaceutical properties, which include high mucoadhesion and a great capacity to produce drug delivery systems, ensuring the biocompatibility of the drug delivery vehicles is a highly relevant issue. Nevertheless, this subject is not addressed as frequently as desired and even though the application of chitosan carriers has been widely explored, the demonstration of systems biocompatibility is still in its infancy. In this review, addressing the biocompatibility of chitosan carriers with application in drug delivery is discussed and the methods used in vitro and in vivo, exploring the effect of different variables, are described. We further provide a discussion on the pros and cons of used methodologies, as well as on the difficulties arising from the absence of standardization of procedures.

  14. Clinical verification of biocompatibility of different dental materials and three filling materials on the interproximal caries of permanent molars%不同口腔修复材料生物相容性及3种材料充填恒磨牙邻面龋的临床验证

    Institute of Scientific and Technical Information of China (English)

    廖伟; 周年苟; 扈祚文; 高军

    2011-01-01

    背景:高强度、良好的适合性、可以接受的美学效果等都是牙科固定修复体所必须的.在材料的具体选择上应根据牙齿缺损的部位、范围以及有无龋坏组织等具体情况去选择.那么,哪一种材料更具有良好的性能及生物相容性呢?目的:从材料学及临床应用方面总结不同口腔修复材料的生物相容性,并评价了玻璃离子水门汀、银汞合金、光固化复合树脂充填恒磨牙邻面龋的临床疗效.方法:以"口腔修复;材料;陶瓷;合金;相容性"为中文关键词;以"interproximal caries; permanent molar; filling" 为英文关键词,采用计算机检索2000-01/2010-10相关文章.纳入与不同材料在口腔修复中的应用及相容性分析相关的文章;排除重复研究或Meta分析类文章.以25篇文献为主重点分析对象讨论了口腔不同修复材料性能的重要性.结果与结论:传统的合金类修复材料普遍存在硬度高、耐磨损等缺点,但因依靠机械固位,且修复洞缘微漏现象明显,必须严格制备洞型;而陶瓷类或粉剂类有黏着性,但硬度低,边缘密台性差.而随着纳米材料与技术的兴起和迅速发展,将口腔材料与纳米材料技术相结合,在引进改性和创新中,形成一种全新的理念、新的材料技术模式,必将在口腔医学领域中取得突破性进展.%BACKGROUND: High strength, good aptness, and acceptable aesthetic effect are all necessary for fixed partial denture (FPD).The specific choice of materials should be based on the site of tooth defects, scope and have or not carious organizations.Therefore, which kind of materials has good performance and biocompatibility?OBJECTIVE: To summarize the biocompatibility of different dental materials from the aspect of hylology and clinical application,and to evaluate the clinical effect of radiopaque glass lonomer luting cement, silver amalgam, light-cured composite filling materials on the interproximal caries of

  15. Preparation and biocompatibility evaluation of composite scaffold material of polyvinyl alcohol/gelatin%多孔聚乙烯醇/明胶软骨组织工程支架复合材料的制备及其生物相容性

    Institute of Scientific and Technical Information of China (English)

    郭涛; 韩志; 杨天府; 李玉宝; 苑天红; 肖杰; 陈艺新

    2011-01-01

    背景:以明胶为基体制备的组织工程支架材料具有良好的生物相容性和生物降解性能,但存在力学性能低,降解速率难以控制的缺陷.目的:制备一种软骨组织工程支架材料多孔聚乙烯醇/明胶复合物,并检测其理化性能和生物相容性.方法:采用乳化发泡法制备聚乙烯醇/明胶多孔支架,并通过电镜分析、力学测试、皮下植入实验,检测材料孔径和孔隙率、IR光谱、力学性能和生物相容性.结果与结论:多孔材料内部呈三维网状多孔结构,孔径均匀,有相似的孔隙率61.8%,含水率44.6%,抗拉强度为(5.01± 0.03) MPa,抗压强度为(1.47±0.36) MPa,有较好的力学性能,IR光谱分析表明材料内部结构均匀.皮下植入后,炎症反应逐渐减轻,囊壁逐渐变薄,并趋于稳定,提示多孔聚乙烯醇/明胶支架材料具有较好的生物相容性和力学性能.%BACKGROUND: The tissue engineered scaffolds prepared with gelatin as base have good biocompatibility and biodegradability,but low mechanical properties and difficulty to control degradation rate are their shortcomings.OBJECTIVE: To prepare a new type of composite scaffold material of polyvinyl alcohol/gelatin and to evaluate the biocompatibility of composite scaffold.METHODS: New type composite materials (polyvinyl alcohol/gelatin) were prepared by foaming method. The pore size and porosity, IR spectroscopy, mechanical performance and biocompatibility were detected by using scanning electron microscope,mechanical test and subcutaneous implantation test.RESULTS AND CONCLUSION: Composite materials (polyvinyl alcohol/gelatin) were three-dimensional porous structure. The pore size was even, and the porosity was 61.8%. The water content rate of the composite materials was 44.6%. The tensile strength was (5.01±0.03) MPa, and the compressive strength was (1.47±0.36) MPa. The new type composite materials have been proved to have good biocompatibility and biomechanical

  16. Enhanced bio-compatibility of ferrofluids of self-assembled superparamagnetic iron oxide-silica core-shell nanoparticles

    Digital Repository Service at National Institute of Oceanography (India)

    Narayanan, T.N.; Mary, A.P.R.; Swalih, P.K.A.; Kumar, D.S.; Makarov, D.; Albrecht, M.; Puthumana, J.; Anas, A.; Anantharaman, A.

    Self-assembled magnetic colloidal suspensions are sought after by material scientists owing to its huge application potential. The biomedical applications of colloidal nanoparticles necessitate that they are biocompatible, non...

  17. Biocompatibility of Ti35Nb3Zr2Ta, a new beta-titanium alloy, as joint prosthesis material%新型β钛合金Ti35Nb3Zr2Ta在人工关节假体应用中的生物相容性

    Institute of Scientific and Technical Information of China (English)

    段永刚; 丁英奇; 张龙; 刘玉章; 唐晓龙

    2015-01-01

    背景:目前被广泛应用于人体关节置换的钛合金为Ti6Al4V,但其弹体模量高于人体骨,导致人工关节假体的稳定性差。而新型β钛合金Ti35Nb3Zr2Ta弹性模量较低,或许能成为新一代生物相容性较好的人体关节假体材料。目的:探讨新型β钛合金Ti35Nb3Zr2Ta在人工关节假体应用的生物相容性。方法:应用计算机检索自万方数据库、中国知网和PubMed文献数据库,检索时间范围2010至2015年,以“新型β钛合金;人工关节假体;生物相容性”为检索词,检索医用人工关节假体材料的应用现状及新型β钛合金Ti35Nb3Zr2Ta在人工关节假体应用的生物相容性的研究。结果与结论:与Ti6Al4V相比,Ti35Nb3Zr2Ta表面粗糙度较高,表面接触角较小,碱性磷酸酶活性以及成骨细胞的钙沉积量明显高于Ti6Al4V,具有良好的生物相容性,可以考虑在人工关节假体领域中进一步广泛应用。%BACKGROUND:Ti6Al4V is a titanium aloy that is widely used in human joint replacement, but its modulus of elasticity is greater than human bone, resulting in the bad stability of the prosthesis. Ti35Nb3Zr2Ta, a new βtitanium aloy, has a lower modulus of elasticity, and maybe becomes a new-generation human joint prosthesis material that has a better biocompatibility. OBJECTIVE:To study the biocompatibility of Ti35Nb3Zr2Ta in prosthesis. METHODS:Wanfang, CNKI and PubMed databases were retrieved using a computer with “new β titanium;prosthesis; biocompatible” as keywords, and the retrieval time ranged from 2010 to 2015. Articles focusing on current application status for medical prosthesis materials and the biocompatibility of Ti35Nb3Zr2Ta in prosthesis were selected. RESULTS AND CONCLUSION:Compared with Ti6Al4V, Ti35Nb3Zr2Ta has higher surface roughness and smaler surface contact angle; the alkaline phosphatase activity and amount of calcium deposits in osteoblasts cultured at Ti35Nb3Zr2Ta

  18. Biocompatible layers fabricated using KrF laser

    Czech Academy of Sciences Publication Activity Database

    Jelínek, Miroslav; Kocourek, Tomáš; Vrbová, M.; Koňařík, D.; Remsa, Jan

    Bellingham: SPIE, 2008 - (Tománek, P.; Senderáková, D.; Hrabovský, M.), 71380G/1-71380G/7. (Proceedings of SPIE. 7138). ISBN 978-0-8194-7379-0. [Photonics Prague 2008 - International Conference on Photonics, Devices and Systems /6./. Prague (CZ), 27.08.2008-29.08.2008] Institutional research plan: CEZ:AV0Z10100522 Keywords : biocompatible materials * thin films * PLD * hydroxyapatite * DLC Subject RIV: BH - Optics, Masers, Lasers http://dx.doi.org/10.1117/12.817980

  19. Clinical verification of biocompatibility of different dental materials and three kinds of material filling molar caries%不同口腔修复材料生物相容性及3种材料充填恒磨牙邻面龋的临床验证

    Institute of Scientific and Technical Information of China (English)

    吴燕

    2014-01-01

    目的:探讨3种不同口腔修复材料用于恒磨牙邻面龋填充的临床疗效。方法选取390例恒磨牙邻面龋患者作为研究对象,随机分为A、B、C三组(各130例),3组分别采用银汞合金、玻璃离子水门汀和光固化复合树脂等3种材料进行治疗。同时使用计算机检索口腔修复材料生物相容性相关文献,检索文献年限范围为2011年1月~2013年10月。根据检索出来的相关文献对不同口腔修复材料的生物相容性进行分析。结果1年复查显示,A组的治疗成功率为91.17%,B组为87.32%,C组为92.81%;3年复查显示,A组的治疗成功率为92.53%,B组为68.75%,C组为87.59%。结论传统合金材料存在硬度高、耐磨损等缺点,而陶瓷类或粉剂类材料有硬度低、边缘密合性差等缺点。而随着医疗技术和纳米技术的飞速发展,为结合纳米材料治疗提供了一种新途径。%Objective To discuss the clinical curative effect of three kinds of different dental materials used for molar caries filling. Methods 390 cases of molar caries patients were selected and randomly divided them into A,B,C three groups(each group of 130 cases).3 groups of patients were treated respectively with silver amalgam,glass incomer cement and light cured composite resin.At the same time,the literature of dental restorative materials biological compatibility was searched by computer(retrieval time:January 2011 to October 2013).According to the related literature retrieval,the biocompatibilities of different dental materials were analyzed. Results After 1 year,the treatment success rate of group A, group B,and group C was 91.17%,87.32%,and 92.81% respectively.After 3 years,the treatment success rate of group A,group B,and group C was 92.53%,68.75%,and 87.59%. Conclusion The traditional alloy material is disadvantageous in high hardness and abrasion resistance.Ceramic or powder material has low hardness and marginal fitness

  20. Fabrication and biocompatibility of polyethyleneimine/heparin self-assembly coating on NiTi alloy

    International Nuclear Information System (INIS)

    NiTi alloy has been used widely as biomaterials. But because of toxic effects possibly caused by excess Ni ions released during the corrosion process in the physiological environment, it is still a controversial material. Fabricating medicine-loaded coating, which is expected to decrease the release of Ni ions and improve the biocompatibility of the materials, is a potential way to solve the problem. In this paper, NiTi alloy is coated by polyethyleneimine/heparin films via layer-by-layer (LBL) self-assembly method. UV-Vis, FT-IR, atomic force microscopy (AFM) and contact angle measurements are used to characterize the microstructure of coatings and select the best fabrication conditions. Potentiodynamic polarization researches in sodium chloride and dynamic clotting time experiment are utilized to study its corrosion resistance capability and biocompatibility of coatings, respectively. The results indicate that PEI/heparin multilayer coating can improve the biocompatibility of NiTi alloy surface

  1. In-situ biopreparation of biocompatible bacterial cellulose/graphene oxide composites pellets

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Wenkun, E-mail: zhuwenkun@swust.edu.cn [State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010 (China); Li, Wei [State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010 (China); He, Yi; Duan, Tao [Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology, Mianyang 621010 (China)

    2015-05-30

    Highlights: • The in situ vibration method was used to synthetize BC/GO composite. • The cytotoxicity assays of BC/GO showed a better biocompatibility. • It is first time to use BC/GO composite for drug delivery. - Abstract: In the present work, a composite material formed by bacterial cellulose (BC) and graphene oxide (GO) was synthesized and characterized. GO was added in the bacteria culture media and then the bacteria was inoculated. The BC/GO pellets composite was prepared into the bacteria culture media and vibrated in Erlenmeyer flask. Characterization of the BC/GO composite showed GO nanosheets embedded in the nanofibers network of BC. The experiments in this study demonstrated BC and GO effectively interacted by hydrogen bonding. Moreover, the cytotoxicity assays showed the material had a better biocompatibility than the counterparts and promoted the cell proliferation excellently. The biocompatibility of BC/GO has the potential to be used for drug delivery.

  2. In-situ biopreparation of biocompatible bacterial cellulose/graphene oxide composites pellets

    International Nuclear Information System (INIS)

    Highlights: • The in situ vibration method was used to synthetize BC/GO composite. • The cytotoxicity assays of BC/GO showed a better biocompatibility. • It is first time to use BC/GO composite for drug delivery. - Abstract: In the present work, a composite material formed by bacterial cellulose (BC) and graphene oxide (GO) was synthesized and characterized. GO was added in the bacteria culture media and then the bacteria was inoculated. The BC/GO pellets composite was prepared into the bacteria culture media and vibrated in Erlenmeyer flask. Characterization of the BC/GO composite showed GO nanosheets embedded in the nanofibers network of BC. The experiments in this study demonstrated BC and GO effectively interacted by hydrogen bonding. Moreover, the cytotoxicity assays showed the material had a better biocompatibility than the counterparts and promoted the cell proliferation excellently. The biocompatibility of BC/GO has the potential to be used for drug delivery

  3. Study on the biodegradability and biocompatibility of WE magnesium alloys

    International Nuclear Information System (INIS)

    Magnesium alloys have been widely applied in biomedical devices because of their high strength, toughness, processing performance and the trace release of Mg2+. In this study, we investigated the biodegradability, cytocompatibility and hemocompatibility of four kinds of WE Mg alloys (where “W” indicates the metallic element Y and “E” represents mixed rare earth [RE] elements; Y: 2.5, 5.0, 6.5, and 7.5 wt.%; Nd: 1.0, 2.5, 2.6, and 4.2 wt.%; Zr: 0.8 wt.%) for their application in intravascular stent fabrication. The content of alloying elements affected not only mechanical properties of materials, but also their biocompatibility. We found that addition of RE elements could reduce the corrosion rates. Human umbilical vein endothelial cells (HUVECs) and vascular smooth muscle cells (VSMCs) were cultured in different extracts of WE Mg alloys. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to examine the cytotoxicity. The nitric oxide (NO) and lactate dehydrogenase (LDH) release were used to evaluate effects of Mg alloys on HUVECs. The addition of Y, Nd and Zr increased the cell viability and improved the hemocompatibility. Different alloy elements affected the morphology of samples, Mg2+ release, and pH values in the medium. The results of mechanical properties, biocompatibility and biodegradability showed that Mg–5.0Y–2.6Nd–0.8Zr might be used as alternative materials of stent. However, it still needs to be further modified for clinical use. These findings suggest that selecting suitable alloying elements is particularly important. - Highlights: ► Y, Nd and Zr are used as alloying elements of magnesium alloys. ► Biodegradability and biocompatibility are explored. ► Cell viability and hemocompatibility are improved as compared with pure Mg.

  4. A pore way to heal and regenerate: 21st century thinking on biocompatibility.

    Science.gov (United States)

    Ratner, Buddy D

    2016-06-01

    This article raises central questions about the definition of biocompatibility, and also about how we assess biocompatibility. We start with the observation that a porous polymer where every pore is spherical, ∼40 microns in diameter and interconnected, can heal into vascularized tissues with little or no fibrosis and good restoration of vascularity (i.e., little or no foreign body reaction). The same polymer in solid form will trigger the classic foreign body reaction characterized by a dense, collagenous foreign body capsule and low vascularity. A widely used definition of biocompatibility is 'the ability of a material to perform with an appropriate host response in a specific application'. With precision-porous polymers, in direct comparison with the same polymer in solid form, we have the same material, in the same application, with two entirely different biological reactions. Can both reactions be 'biocompatible?' This conundrum will be elaborated upon and proposals will be made for future considerations and measurement of biocompatibility. PMID:27047676

  5. Biocompatibility of microplates for culturing epithelial renal cells evaluated by a microcalorimetric technique.

    Science.gov (United States)

    Xie, Y; DePierre, J W; Nässberger, L

    2000-09-01

    In the present study we have developed a microcalorimetric procedure which allows convenient investigation of biocompatibility in a microsystem. We examined the biocompatibility of a porcine renal epithelial tubule cell line LLC-PK1 and a human primary renal epithelial tubule cell (RPTEC) with microplates composed of three different materials, i.e. Thermanox, transparent film and titanium. All three materials showed equal biocompatibility with LLC-PK1 cells, judging from the attainment of steady-state power curves and the same rate of heat production per cell (2.5 microW / microg DNA). The human renal cells were poorly biocompatible with the Thermanox and transparent film. However, on titanium the RPTEC cell did adhere, as demonstrated by a steady-state power curve. The human cells also showed a higher metabolic activity (3.0 microW / microg DNA), than did LLC-PK1 cells cultured on the same type of microplates. In research on biocompatibility there is a need for alternatives to experimental animal investigations. The present technique allows studies of cellular interactions with different biomaterials in a rapid and standardized manner and may therefore prove to be a useful screening procedure. PMID:15348389

  6. Comparison of in vivo biocompatibilities between parylene-C and polydimethylsiloxane for implantable microelectronic devices

    Indian Academy of Sciences (India)

    Dong Sup Lee; Su Jin Kim; Eun Bi Kwon; Cheol Whee Park; Su Min Jun; Bumkyoo Choi; Sae Woong Kim

    2013-11-01

    Implantable devices are often composed of or coated with different biologically compatible materials based on their requirements. Selecting a surface material for an implantable device is not an easy task, and it is necessary to compare the biocompatibilities of the available surface materials. In this study, we perform a comparison of the in vivo biocompatibilities of polydimethylsiloxane (PDMS) and para-xylyene polymer (parylene-C) as they are considered to be candidates for a coating material for implantable microelectronic devices. For in vivo biocompatibility testing, fifty four male Sprague-Dawley rats were used for testing, and they were divided into three groups (PDMS, parylene-C and a positive control). At one, four and twelve weeks after implantation of the test object, the density of inflammatory cells and the granulation layer thickness were recorded for each group and compared with other groups using visible light and fluorescence microscopy. The thickness of the granulation layer tended to decrease over time for all of the experimental groups, whereas the granulation layer thickness remained constant in the positive control group. The thinnest capsular layer was observed for the parylene-C group and fewest inflammatory cells were observed in this group during the entire experimental period. Macrophage infiltration was minimal, even at one week, and was not observed thereafter. The parylene-C group showed better biocompatibility than the PDMS groups, both for acute and chronic implantation. Thus, parylene-C is the best candidate of the tested materials for applications involving permanent implantable micro-devices.

  7. Hydrophilic polymers - biocompatibility testing in vitro

    Czech Academy of Sciences Publication Activity Database

    Kejlová, K.; Labský, Jiří; Jírová, D.; Bendová, H.

    Warsaw : Dept.Transpl & CBT , Centre of Biostructure Research, Medical University of Warsaw, 2004. s. 71. [Workshop of the European Society of Toxicology In Vitro: Invitox /13./. 08.09.2004-11.09.2004, Zegrze] R&D Projects: GA AV ČR IAA4050301 Keywords : hydrophilic polymers * biocompatibility * cytotoxicity Subject RIV: CD - Macromolecular Chemistry

  8. Polymeric barrier membranes for device packaging, diffusive control and biocompatibility

    International Nuclear Information System (INIS)

    Current state-of-the-art implantable micron feature electronic devices are capable of monitoring and stimulating functions in vivo. Within an EU Framework VI project a further step was taken in developing key microsystem technologies and communication methods that could bring intelligence directly to the human interface, in the form of reactive medical implants and ambulatory measurement systems. Information from these devices is planned to be transmitted out into the wider environment for remote processing. However, the packaging of such state-of-the-art devices to enhance tissue biocompatibility, and to protect conducting elements from in vivo corrosion during extended use, along with protecting the body from toxins leaching from implant components, remains a concern. Candidate polymeric barriers as hydration resistant and solute impermeable interfaces to mitigate such major problems of chronic implantation were investigated. Materials studied included silicone rubber, PVC, polyurethane, and diamond-like carbon (DLC). Polymer permeability to water solutes was marginally improved through incorporation of lipid into these structures. Surface biocompatibility was assessed on the basis of protein film deposition in vitro and by cell viability studies in tissue culture. Short-term toxicity was not observed for any of the tested materials, though there were substantial differences in hydration. Additionally, polypyrrole over active electrodes shows feasibility for controlled tissue interfacing whilst retaining electrical conductivity

  9. Biocompatible composites for orthopedics applications

    International Nuclear Information System (INIS)

    The paper presents the results of the experimental research on the laminated composites and on that with the microconcretes structure destined for medical applications. These composites were based on the porous aluminia ceramics. Many materials with very different physicomechanical features were obtained. They may serve as the implants for filling bone cavities, and some of them even in the places where act the considerable forces. (author)

  10. Titanium surface modification by using microwave-induced argon plasma in various conditions to enhance osteoblast biocompatibility

    OpenAIRE

    Seon, Gyeung Mi; Seo, Hyok Jin; Kwon, Soon Young; Lee, Mi Hee; Kwon, Byeong-Ju; Kim, Min Sung; Koo, Min-Ah; Park, Bong Joo; Park, Jong-Chul

    2015-01-01

    Background Titanium is a well proven implantable material especially for osseointegratable implants by its biocompatibility and anti-corrosive surface properties. Surface characteristics of the implant play an important role for the evolution of bone tissue of the recipient site. Among the various surface modification methods, plasma treatment is one of the promising methods for enhance biocompatibility. We made microwave-induced argon plasma at atmospheric pressure to improve in titanium sur...

  11. Detecção da citotoxicidade de materiais biocompatíveis nas linhagens celulares MRC-5, HeLa e RC-IAL MRC-5, HeLa and RC-IAL cell lines sensitivity for detection of cytotoxicity of biocompatible materials

    Directory of Open Access Journals (Sweden)

    Aurea S. Cruz

    1992-04-01

    Full Text Available A sensibilidade de uma linhagem celular diplóide e duas heteroplóides, para a detecção de citotoxicidade através do método de difusão em camada de ágar sobre culturas celulares, foi avaliada experimentalmente com solução de ácido ascórbico em diferentes concentrações e, na prática, frente a 562 amostras de 21 diferentes materiais industriais enviados para análise na Seção de Culturas Celulares do Instituto Adolfo Lutz. A linhagem celular heteroplóide designada RC-IAL apresentou, em relação às linhagens MRC-5 e HeLa, maior sensibilidade porque revelou a presença de efeito citotóxico nas menores concentrações utilizadas (10 e 25 ug/ml do ácido ascórbico e apresentou maior diâmetro do halo citotóxico em 15 amostras e igual diâmetro em 16 das 43 amostras (7,6% que resultaram positivas. Nas 43 amostras positivas, a linhagem MRC-5 não revelou citotoxicidade em 3 amostras de espuma e 1 de resina acrílica. O polivinilcloreto (PVC e o polietileno, raramente revelaram positividade, enquanto plástico, algodão e resinas acrílicas revelaram citotoxicidade ao redor de 5%. Em vista dos resultados é discutida a proposta da utilização da linhagem RC-IAL e HeLa para a continuidade das futuras análises solicitadas ao Instituto Adolfo LutzThe sensitivity of diploid and heteroploid cell lines for detection of cytotoxicity using the agar diffusion method on cell culture, was tested with ascorbic acid solution of different concentrations. A total of 562 samples of 21 various materials were tested. The heteroploid cell line, RC-IAL, showed in relation to the MRC-5 and HeLa cell lines, greater sensitivity because it showed the presence of cytotoxic effect with the lowest concentration used (10 and 25ug/ml of ascorbic acid and showed greater diameter of cytotoxic halo in 15 samples and equal diameter in 16 of the 43 positive samples (7.6%. Out of 43 positive samples, the MRC-5 line did not show cytotoxicity in 3 sponge samples and

  12. Plasma nitriding and duplex coating of biocompatible surfaces for low friction and wear resistant applications

    OpenAIRE

    Rahman, Md. Mahfujur

    2006-01-01

    The 316L austenitic stainless steels, Ti-6A1-4V and Ultrahigh molecular weight polyethylene (UHMWP) are very attractive and well known materials for diverse engineering applications due to their many superior properties. More specifically, these materials have acquired much attention in biomedical applications due to their excellent biocompatibility. However, the practical use of these materials are limited in many cases by the need to attain specific tribological (that means, low' friction a...

  13. In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy

    OpenAIRE

    Dimić Ivana; Cvijović-Alagić Ivana; Obradović Nataša; Petrović Jelena; Putić Slaviša; Rakin Marko; Bugarski Branko

    2015-01-01

    Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity which represents ideal environment for metallic ion release and biodegradation. The released metallic ions from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, the dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic mater...

  14. Thermodynamics of non-bridging oxigen in silica bio-compatible glass-ceramics

    Czech Academy of Sciences Publication Activity Database

    Koga, N.; Strnad, Z.; Šesták, Jaroslav; Strnad, J.

    2003-01-01

    Roč. 71, - (2003), s. 927-937. ISSN 1418-2874 R&D Projects: GA AV ČR IAA4010101 Institutional research plan: CEZ:AV0Z1010914 Keywords : bio-compatible * bone-like apatite * glass-ceramics * mimetic material * thermodynamic s Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 0.598, year: 2002

  15. Influence of mechanical instruments on the biocompatibility of titanium dental implants surfaces: a systematic review

    NARCIS (Netherlands)

    A. Louropoulou; D.E. Slot; F. van der Weijden

    2015-01-01

    Objective The objective of this systematic review was to evaluate the effect of mechanical instruments on the biocompatibility of titanium dental implant surfaces. Materials and methods MEDLINE, Cochrane-CENTRAL and EMBASE databases were searched up to December 2013, to identify controlled studies o

  16. Mechanical strength and biocompatibility of ultrafine-grained commercial purity titanium.

    Science.gov (United States)

    Estrin, Yuri; Kim, Hyoun-Ee; Lapovok, Rimma; Ng, Hoi Pang; Jo, Ji-Hoon

    2013-01-01

    The effect of grain refinement of commercial purity titanium by equal channel angular pressing (ECAP) on its mechanical performance and bone tissue regeneration is reported. In vivo studies conducted on New Zealand white rabbits did not show an enhancement of biocompatibility of ECAP-modified titanium found earlier by in vitro testing. However, the observed combination of outstanding mechanical properties achieved by ECAP without a loss of biocompatibility suggests that this is a very promising processing route to bioimplant manufacturing. The study thus supports the expectation that commercial purity titanium modified by ECAP can be seen as an excellent candidate material for bone implants suitable for replacing conventional titanium alloy implants. PMID:23936857

  17. Biocompatibility and Toxicity of Poly(vinyl alcohol)/N,O-Carboxymethyl Chitosan Scaffold

    OpenAIRE

    Tunku Kamarul; Krishnamurithy, G.; Salih, Noman D.; Nurul Syuhada Ibrahim; Hanumantha Rao Balaji Raghavendran; Abdul Razzaq Suhaeb; D. S. K. Choon

    2014-01-01

    The in vivo biocompatibility and toxicity of PVA/NOCC scaffold were tested by comparing them with those of a biocompatible inert material HAM in a rat model. On Day 5, changes in the blood parameters of the PVA/NOCC-implanted rats were significantly higher than those of the control. The levels of potassium, creatinine, total protein, A/G, hemoglobulin, erythrocytes, WBC, and platelets were not significantly altered in the HAM-implanted rats, when compared with those in the control. On Day 10,...

  18. Biocompatibility of magnesium implants in primary human reaming debris-derived cells stem cells in vitro

    OpenAIRE

    Charyeva, Olga; Dakischew, Olga; Sommer, Ursula; Heiss, Christian; Schnettler, Reinhard; Lips, Katrin Susanne

    2015-01-01

    Background Use of magnesium for resorbable metal implants is a new concept in orthopaedic and dental medicine. The majority of studies on magnesium’s biocompatibility in vitro have assessed the short-term effect of magnesium extract on cells. The aim of this study was to evaluate the influence of direct exposure to magnesium alloys on the bioactivity of primary human reaming debris-derived (HRD) cells. Materials and methods Pure Mg, Mg2Ag, WE43 and Mg10Gd were tested for biocompatibility. The...

  19. 3D Printing of Biocompatible Supramolecular Polymers and their Composites.

    Science.gov (United States)

    Hart, Lewis R; Li, Siwei; Sturgess, Craig; Wildman, Ricky; Jones, Julian R; Hayes, Wayne

    2016-02-10

    A series of polymers capable of self-assembling into infinite networks via supramolecular interactions have been designed, synthesized, and characterized for use in 3D printing applications. The biocompatible polymers and their composites with silica nanoparticles were successfully utilized to deposit both simple cubic structures, as well as a more complex twisted pyramidal feature. The polymers were found to be not toxic to a chondrogenic cell line, according to ISO 10993-5 and 10993-12 standard tests and the cells attached to the supramolecular polymers as demonstrated by confocal microscopy. Silica nanoparticles were then dispersed within the polymer matrix, yielding a composite material which was optimized for inkjet printing. The hybrid material showed promise in preliminary tests to facilitate the 3D deposition of a more complex structure. PMID:26766139

  20. Biocompatible Coating of Encapsulated Cells Using Ionotropic Gelation

    Science.gov (United States)

    Ehrhart, Friederike; Mettler, Esther; Böse, Thomas; Weber, Matthias Max; Vásquez, Julio Alberto; Zimmermann, Heiko

    2013-01-01

    The technique of immunoisolated transplantation has seen in the last twenty years improvements in biocompatibility, long term stability and methods for avoidance of fibrosis in alginate capsules. However, two major problems are not yet solved: living cellular material that is not centered in the capsule is not properly protected from the hosts’ immune system and the total transplant volume needs to be reduced. To solve these problems, we present a method for applying fully biocompatible alginate multilayers to a barium-alginate core without the use of polycations. We report on the factors that influence layer formation and stability and can therefore provide data for full adjustability of the additional layer. Although known for yeast and plant cells, this technique has not previously been demonstrated with mammalian cells or ultra-high viscous alginates. Viability of murine insulinoma cells was investigated by live-dead staining and live cell imaging, for murine Langerhans’ islets viability and insulin secretion have been measured. No hampering effects of the second alginate layer were found. This multi-layer technique therefore has great potential for clinical and in vitro use and is likely to be central in alginate matrix based immunoisolated cell therapy. PMID:24039964

  1. Preparation of biocompatible structural gradient coatings on pure titanium

    Institute of Scientific and Technical Information of China (English)

    TANG Guang-xin; ZHANG Ren-ji; YAN Yong-nian

    2004-01-01

    In order to overcome the poor osteo-inductive properties of titanium implant, some methods have been used. The efforts to improve implant biocompatibility and durability by applying a hybrid technique of composite oxidation (pre-anodic and micro-arc oxidation) and hydrothermal treatment were described. Pure titanium was used as the substrate material. An oxalic acid was used as the electrolyte for the pre-anodic oxidation. A calcium and phosphate salt solution was acted as the electrolyte of micro-arc oxidation and the common pure water was used for hydrothermal treatment. X-ray diffraction (XRD), and scanning electron microscopy (SEM) have been used to investigate the microstructure and morphology of the coatings. The results show that a compact TiO2 film can be made by pre-anodic oxidation, which is effective as chemical barriers against the in-vivo release of metal ions from the implants. A porous TiO2 coating can be produced by micro-arc oxidation on titanium plate, which is beneficial to bone tissue growth and enhancing anchorage of implant to bone. De-calcium HA can be formed on the coating using hydrothermal treatment, which is similar with the primary component of bone and has a very good osteo-inductivity.The porous gradient titania coating made by the hybrid oxidation and hydrothermal treatment should show good biocompatibility in the environment of the human body.

  2. Ion implantation and bio-compatibility

    International Nuclear Information System (INIS)

    Surface modification of polymers by ion implantation has been carried out to control surface properties such as conductivity, wettability, blood and tissue compatibility. Ion implantation into silicone rubber, polystyrene and segmented polyurethane was performed at 150 keV with doses ranging from 1 x 1015 to 3 x 1017 ions/cm2 to improve bio-compatibility. The platelet accumulation on ion implanted silicone rubber decreased and non-thrombogenicity of ion implanted specimens were improved. The ion implanted polystyrene and segmented polyurethane have been found to exhibit remarkably higher adhesion and spreading of endothelial cells compared to the non-implanted case. It is concluded that ion implantation into polymers is effective in controlling their bio-compatibility. (author)

  3. In vivo qualitative analysis of the biocompatibility of different cyanoacrylate-based adhesives

    Directory of Open Access Journals (Sweden)

    Rafael Tobias Moretti Neto

    2008-03-01

    Full Text Available Cyanocrylates have been widely used in the medical and dental fields for several years. In Dentistry, cyanoacrylates have been used for suturing, pulp capping, as retrofilling material in endodontic surgeries, and as cervical plug for pulpless teeth bleaching. The biocompatibility of these adhesives has been the topic of many researches and subcutaneous implantation is an effective methodology for these studies. The present study evaluated the biocompatibility of three different cyanoacrylate-based adhesives. Thirty-six Wistar rats were used, divided into four groups of 9 animals each: A (control - distilled water, B - cyanoacrylate ester (Super Bonder, C - n-butyl-cyanoacrylate (Histoacryl and D - alpha-cyanoacrylate (Three Bond. The materials were dispensed in sponges of polyvinyl chloride, the animals were incised and the sponges were inserted in the subcutaneous tissue and sutured. Each group was sub-divided according to the time of sacrifice of the animals: 7, 21 and 45 days. Subjective analysis of the histologic material showed that all groups presented some degree of irritability, but the inflammatory reaction decreased with the experimental time in all groups. Group D showed an inflammatory reaction which was closer to that of the control group and was considered to have good biocompatibility. Groups B and C were similar and presented more aggressive inflammatory reactions when compared to the control group. Based on the results, it was concluded that alpha-cyanoacrylate (Three Bond was the most biocompatible adhesive because it caused the lowest levels of inflammation.

  4. Physicochemical characterization and biocompatibility of alginate-polycation microcapsules designed for islet transplantation

    Science.gov (United States)

    Tam, Susan Kimberly

    Microencapsulation represents a method for immunoprotecting transplanted therapeutic cells or tissues from graft rejection using a physical barrier. This approach is advantageous in that it eliminates the need to induce long-term immunosuppression and allows the option of transplanting non-cadaveric cell sources, such as animal cells and stem cell-derived tissues. The microcapsules that we have investigated are designed to immunoprotect islets of Langerhans (i.e. clusters of insulin-secreting cells), with the goal of treating insulin-dependent diabetes. With the aid of techniques for physicochemical analysis, this research focused on understanding which properties of the microcapsule are the most important for determining its biocompatibility. The objective of this work was to elucidate correlations between the chemical make-up, physicochemical properties, and in vivo biocompatibility of alginate-based microcapsules. Our approach was based on the hypothesis that the immune response to the microcapsules is governed by, and can therefore be controlled by, specific physicochemical properties of the microcapsule and its material components. The experimental work was divided into five phases, each associated with a specific aim : (1) To prove that immunoglobulins adsorb to the surface of alginate-polycation microcapsules, and to correlate this adsorption with the microcapsule chemistry. (2) To test interlaboratory reproducibility in making biocompatible microcapsules, and evaluate the suitability of our materials and fabrication protocols for subsequent studies. (3) To determine which physicochemical properties of alginates affect the in vivo biocompatibility of their gels. (4) To determine which physiochemical properties of alginate-polycation microcapsules are most important for determining their in vivo biocompatibility (5) To determine whether a modestly immunogenic membrane hinders or helps the ability of the microcapsule to immunoprotect islet xenografts in

  5. Osseointegration and biocompatibility of different metal implants - a comparative experimental investigation in sheep

    OpenAIRE

    Plecko Michael; Sievert Christine; Andermatt Daniel; Frigg Robert; Kronen Peter; Klein Karina; Stübinger Stefan; Nuss Katja; Bürki Alexander; Ferguson Stephen; Stoeckle Ulrich; von Rechenberg Brigitte

    2012-01-01

    Abstract Background In the present study, 4 different metallic implant materials, either partly coated or polished, were tested for their osseointegration and biocompatibility in a pelvic implantation model in sheep. Methods Materials to be evaluated were: Cobalt-Chrome (CC), Cobalt-Chrome/Titanium coating (CCTC), Cobalt-Chrome/Zirconium/Titanium coating (CCZTC), Pure Titanium Standard (PTST), Steel, TAN Standard (TANST) and TAN new finish (TANNEW). Surgery was performed on 7 sheep, with 18 i...

  6. Osseointegration and biocompatibility of different metal implants--a comparative experimental investigation in sheep

    OpenAIRE

    Plecko, Michael; Sievert, Christine; Andermatt, Daniel; Frigg, Robert; Kronen, Peter W; Klein, Karina; Stübinger, Stefan; Nuss, Katja M; Bürki, Alexander; Ferguson, Stephen; Stoeckle, Ulrich; von Rechenberg, Brigitte

    2012-01-01

    BACKGROUND: In the present study, 4 different metallic implant materials, either partly coated or polished, were tested for their osseointegration and biocompatibility in a pelvic implantation model in sheep. METHODS: Materials to be evaluated were: Cobalt-Chrome (CC), Cobalt-Chrome/Titanium coating (CCTC), Cobalt-Chrome/Zirconium/Titanium coating (CCZTC), Pure Titanium Standard (PTST), Steel, TAN Standard (TANST) and TAN new finish (TANNEW). Surgery was performed on 7 sheep, with 18 impla...

  7. Osseointegration and biocompatibility of different metal implants-a comparative experimental investigation in sheep

    OpenAIRE

    Plecko, Michael; Sievert, Christine; Andermatt, Daniel; Frigg, Robert; Kronen, Peter; Klein, Karina; Stübinger, Stefan; Nuss, Katja; Bürki, Alexander; Ferguson, Stephen; Stoeckle, Ulrich; von Rechenberg, Brigitte

    2012-01-01

    Background In the present study, 4 different metallic implant materials, either partly coated or polished, were tested for their osseointegration and biocompatibility in a pelvic implantation model in sheep. Methods Materials to be evaluated were: Cobalt-Chrome (CC), Cobalt-Chrome/Titanium coating (CCTC), Cobalt-Chrome/Zirconium/Titanium coating (CCZTC), Pure Titanium Standard (PTST), Steel, TAN Standard (TANST) and TAN new finish (TANNEW). Surgery was performed on 7 sheep, with 18...

  8. Effect of Processing on Silk-Based Biomaterials: Reproducibility and Biocompatibility

    OpenAIRE

    Wray, Lindsay S.; Hu, Xiao; Gallego, Jabier; Georgakoudi, Irene; Omenetto, Fiorenzo G.; Schmidt, Daniel; Kaplan, David L.

    2011-01-01

    Silk fibroin has been successfully used as a biomaterial for tissue regeneration. In order to prepare silk fibroin biomaterials for human implantation a series of processing steps are required to purify the protein. Degumming to remove inflammatory sericin is a crucial step related to biocompatibility and variability in the material. Detailed characterization of silk fibroin degumming is reported. The degumming conditions significantly affected cell viability on the silk fibroin material and ...

  9. Biocompatibility of nanostructured boron doped diamond for the attachment and proliferation of human neural stem cells

    OpenAIRE

    Taylor, A C; Vagaska, B.; Edgington, R.; Hébert, C.; Ferretti, P.; Bergonzo, P.; Jackman, R. B.

    2015-01-01

    OBJECTIVE: We quantitatively investigate the biocompatibility of chemical vapour deposited (CVD) nanocrystalline diamond (NCD) after the inclusion of boron, with and without nanostructuring. The nanostructuring method involves a novel approach of growing NCD over carbon nanotubes (CNTs) that act as a 3D scaffold. This nanostructuring of BNCD leads to a material with increased capacitance, and this along with wide electrochemical window makes BNCD an ideal material for neural interface applica...

  10. Surface Modification of Biodegradable Polymers towards Better Biocompatibility and Lower Thrombogenicity

    OpenAIRE

    Rudolph, Andreas; Teske, Michael; Illner, Sabine; Kiefel, Volker; Sternberg, Katrin; Grabow, Niels; Wree, Andreas; Hovakimyan, Marina

    2015-01-01

    Purpose Drug-eluting stents (DES) based on permanent polymeric coating matrices have been introduced to overcome the in stent restenosis associated with bare metal stents (BMS). A further step was the development of DES with biodegradable polymeric coatings to address the risk of thrombosis associated with first-generation DES. In this study we evaluate the biocompatibility of biodegradable polymer materials for their potential use as coating matrices for DES or as materials for fully bioabso...

  11. Controllable synthesis of functional nanocomposites: Covalently functionalize graphene sheets with biocompatible L-lysine

    International Nuclear Information System (INIS)

    Highlights: ► The biocompatible L-lysine functionalized graphene sheets (Gs/Lys) were synthesized controllably using a novel method. ► The Gs/Lys nanocomposites are water-soluble, biocompatible and chiral. ► A chiral graphene derivative was proposed. - Abstract: In this paper a novel method to synthesize functionalize graphene sheets (Gs) by biocompatible L-lysine (Gs/Lys) is reported. The method was composed of two steps: (1) we controllably synthesized self-assembly Gs/Lys-Cu-Lys through the terminal amino of copper L-lysine (Lys-Cu-Lys) attaching to graphite oxide (GO) and then reducing. (2) Obtained the Gs/Lys by eliminating the copper ion. This method could also be used to functionalize other nanomaterials by L-lysine. The Gs/Lys nanocomposites are water-soluble, biocompatible, and above all, it is a chiral material of graphene, which is proposed by us. This novel material will be promising for more applications of graphene. The formation of Gs/Lys nanocomposites were confirmed by scanning electron microscopy (SEM), Fourier-transform infrared spectra (FT-IR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and thermal gravimetric (TG) analysis.

  12. Short-and long-term neural biocompatibility of heparin coated sapphire implants

    Energy Technology Data Exchange (ETDEWEB)

    Wang Anfeng [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); McAllister, James P. [Department of Neurological Surgery, Wayne State University, 4201 Antoine Street, UHC-6E, Detroit, MI 48201 (United States); Finlayson, Paul [Department of Otolaryngology, Wayne State University, 550 E Canfield, Lande Room 327, Detroit, MI 48201 (United States); Li, Jie [Department of Neurological Surgery, Wayne State University, 4201 Antoine Street, UHC-6E, Detroit, MI 48201 (United States); Brabant, Kelley [Department of Neurological Surgery, Wayne State University, 4201 Antoine Street, UHC-6E, Detroit, MI 48201 (United States); Tang Haiying [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Black, Carolyn [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Department of Neurological Surgery, Wayne State University, 4201 Antoine Street, UHC-6E, Detroit, MI 48201 (United States); Cao Ting [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Liang Xuemei [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Salley, Steven O. [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Auner, Gregory W. [Department of Electrical and Computer Engineering, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States); Ng, K.Y. Simon [Department of Chemical Engineering and Materials Science, Wayne State University, 5050 Anthony Wayne Drive, Detroit, MI 48202 (United States)]. E-mail: sng@wayne.edu

    2007-03-15

    Sapphire is one of the most promising materials for the development of implantable biomedical devices due to its exceptional chemical, mechanical, electrical, thermal and optical properties. Silicon has also been widely used to manufacture neuroprosthetic devices in the past. However, both of these materials have been found to cause the most severe tissue reactions while implanted in vivo in the rat brain, compared with other biomaterials. In order to enhance the biocompatibility of sapphire and silicon, their surfaces were modified by depositing a self-assembled monolayer (SAM) of octadecyltrichlorosilane (OTS), followed by the photo-immobilization of heparin. To comprehensively evaluate the short- and long-term neural biocompatibility, sapphire and silicon wafers (2.5 mm dia x 0.25 mm thick) with and without heparin coating were implanted on the surface of adult rat's cortex for 10, 28 and 90 days. Specific evaluations of the cell types that contribute to an inflammatory response were performed. The histological results indicate that the biocompatibility of sapphire is dramatically improved by heparin immobilization, while this dramatic improvement is not observed on heparin coated silicon. The failure to improve the biocompatibility of silicon by heparin immobilization can be attributed to the corrosion of the silicon surface in vivo, which was confirmed by atomic force microscopy (AFM). Meanwhile, no corrosion was observed on heparin coated sapphire surfaces and a very thin layer of proteins or extracellular matrix was deposited on the surfaces.

  13. Short-and long-term neural biocompatibility of heparin coated sapphire implants

    International Nuclear Information System (INIS)

    Sapphire is one of the most promising materials for the development of implantable biomedical devices due to its exceptional chemical, mechanical, electrical, thermal and optical properties. Silicon has also been widely used to manufacture neuroprosthetic devices in the past. However, both of these materials have been found to cause the most severe tissue reactions while implanted in vivo in the rat brain, compared with other biomaterials. In order to enhance the biocompatibility of sapphire and silicon, their surfaces were modified by depositing a self-assembled monolayer (SAM) of octadecyltrichlorosilane (OTS), followed by the photo-immobilization of heparin. To comprehensively evaluate the short- and long-term neural biocompatibility, sapphire and silicon wafers (2.5 mm dia x 0.25 mm thick) with and without heparin coating were implanted on the surface of adult rat's cortex for 10, 28 and 90 days. Specific evaluations of the cell types that contribute to an inflammatory response were performed. The histological results indicate that the biocompatibility of sapphire is dramatically improved by heparin immobilization, while this dramatic improvement is not observed on heparin coated silicon. The failure to improve the biocompatibility of silicon by heparin immobilization can be attributed to the corrosion of the silicon surface in vivo, which was confirmed by atomic force microscopy (AFM). Meanwhile, no corrosion was observed on heparin coated sapphire surfaces and a very thin layer of proteins or extracellular matrix was deposited on the surfaces

  14. Motion Control of Urea-Powered Biocompatible Hollow Microcapsules.

    Science.gov (United States)

    Ma, Xing; Wang, Xu; Hahn, Kersten; Sánchez, Samuel

    2016-03-22

    The quest for biocompatible microswimmers powered by compatible fuel and with full motion control over their self-propulsion is a long-standing challenge in the field of active matter and microrobotics. Here, we present an active hybrid microcapsule motor based on Janus hollow mesoporous silica microparticles powered by the biocatalytic decomposition of urea at physiological concentrations. The directional self-propelled motion lasts longer than 10 min with an average velocity of up to 5 body lengths per second. Additionally, we control the velocity of the micromotor by chemically inhibiting and reactivating the enzymatic activity of urease. The incorporation of magnetic material within the Janus structure provides remote magnetic control on the movement direction. Furthermore, the mesoporous/hollow structure can load both small molecules and larger particles up to hundreds of nanometers, making the hybrid micromotor an active and controllable drug delivery microsystem. PMID:26863183

  15. Effect of chitosan/type I collagen/gelatin composites in biocompatibility and nerve repair

    Institute of Scientific and Technical Information of China (English)

    Qing Wang; Xiaolei Yang; Ming Ren; Yulin Hu; Qiang Chen; Lei Xing; Chunyang Meng; Tiemei Liu

    2012-01-01

    Chitosan, collagen I and gelatin were mixed in appropriate quantities to develop a new nerve repair material, with good arrangement and structure, as well as even aperture size. The composite material was sterilized by 60Co irradiation for 24 hours prior to implantation in the right thigh of rats following sciatic nerve damage. Results showed that the material was nontoxic to the kidneys and the liver, and did not induce an inflammatory response in the muscles. The composite material enhanced the recovery of sciatic nerve damage in rats. These experimental findings indicate that the composite material offers good biocompatibility and has a positive effect on injured nerve rehabilitation.

  16. Current and future biocompatibility aspects of biomaterials for hip prosthesis

    Directory of Open Access Journals (Sweden)

    Amit Aherwar

    2015-12-01

    Full Text Available The field of biomaterials has turn into an electrifying area because these materials improve the quality and longevity of human life. The first and foremost necessity for the selection of the biomaterial is the acceptability by human body. However, the materials used in hip implants are designed to sustain the load bearing function of human bones for the start of the patient’s life. The most common classes of biomaterials used are metals, polymers, ceramics, composites and apatite. These five classes are used individually or in combination with other materials to form most of the implantation devices in recent years. Numerous current and promising new biomaterials i.e. metallic, ceramic, polymeric and composite are discussed to highlight their merits and their frailties in terms of mechanical and metallurgical properties in this review. It is concluded that current materials have their confines and there is a need for more refined multi-functional materials to be developed in order to match the biocompatibility, metallurgical and mechanical complexity of the hip prosthesis.

  17. High hardness in the biocompatible intermetallic compound β-Ti3Au.

    Science.gov (United States)

    Svanidze, Eteri; Besara, Tiglet; Ozaydin, M Fevsi; Tiwary, Chandra Sekhar; Wang, Jiakui K; Radhakrishnan, Sruthi; Mani, Sendurai; Xin, Yan; Han, Ke; Liang, Hong; Siegrist, Theo; Ajayan, Pulickel M; Morosan, E

    2016-07-01

    The search for new hard materials is often challenging, but strongly motivated by the vast application potential such materials hold. Ti3Au exhibits high hardness values (about four times those of pure Ti and most steel alloys), reduced coefficient of friction and wear rates, and biocompatibility, all of which are optimal traits for orthopedic, dental, and prosthetic applications. In addition, the ability of this compound to adhere to ceramic parts can reduce both the weight and the cost of medical components. The fourfold increase in the hardness of Ti3Au compared to other Ti-Au alloys and compounds can be attributed to the elevated valence electron density, the reduced bond length, and the pseudogap formation. Understanding the origin of hardness in this intermetallic compound provides an avenue toward designing superior biocompatible, hard materials. PMID:27453942

  18. High hardness in the biocompatible intermetallic compound β-Ti3Au

    Science.gov (United States)

    Svanidze, Eteri; Besara, Tiglet; Ozaydin, M. Fevsi; Tiwary, Chandra Sekhar; Wang, Jiakui K.; Radhakrishnan, Sruthi; Mani, Sendurai; Xin, Yan; Han, Ke; Liang, Hong; Siegrist, Theo; Ajayan, Pulickel M.; Morosan, E.

    2016-01-01

    The search for new hard materials is often challenging, but strongly motivated by the vast application potential such materials hold. Ti3Au exhibits high hardness values (about four times those of pure Ti and most steel alloys), reduced coefficient of friction and wear rates, and biocompatibility, all of which are optimal traits for orthopedic, dental, and prosthetic applications. In addition, the ability of this compound to adhere to ceramic parts can reduce both the weight and the cost of medical components. The fourfold increase in the hardness of Ti3Au compared to other Ti–Au alloys and compounds can be attributed to the elevated valence electron density, the reduced bond length, and the pseudogap formation. Understanding the origin of hardness in this intermetallic compound provides an avenue toward designing superior biocompatible, hard materials.

  19. Biocompatibility of poly allylamine synthesized by plasma

    International Nuclear Information System (INIS)

    A study of the electric and hydrophilic properties of poly allylamine (PAI) synthesized by plasma whose structure contains N-H, C-H, C-O and O-H bonds is presented, that promote the biocompatibility with the human body. To study the PAI hydrolytic affinity, solutions of salt concentration similar to those of the human body were used. The results indicate that the solutions modify the charge balance in the surfaces reducing the hydrophobicity in the poly allylamine whose contact angle oscillates among 10 and 16 degrees and the liquid-solid surface tension between 4 and 8 dina/cm. (Author)

  20. Titanium nanostructural surface processing for improved biocompatibility

    International Nuclear Information System (INIS)

    X-ray photoelectron spectroscopy, grazing incident x-ray diffraction, transmission electron microscopy, and scanning electron microscopy were conducted to evaluate the effect of titanium hydride on the formation of nanoporous TiO2 on Ti during anodization. Nano-titanium-hydride was formed cathodically before anodizing and served as a sacrificial nanoprecipitate during anodization. Surface oxidation occurred and a multinanoporous structure formed after cathodic pretreatments followed by anodization treatment. The sacrificial nanoprecipitate is directly dissolved and the Ti transformed to nanoporous TiO2 by anodization. The formation of sacrificial nanoprecipitates by cathodic pretreatment and of the multinanostructure by anodization is believed to improve biocompatibility, thereby promoting osseointegration

  1. Physicochemical characterization and biocompatibility of alginate-polycation microcapsules designed for islet transplantation

    Science.gov (United States)

    Tam, Susan Kimberly

    Microencapsulation represents a method for immunoprotecting transplanted therapeutic cells or tissues from graft rejection using a physical barrier. This approach is advantageous in that it eliminates the need to induce long-term immunosuppression and allows the option of transplanting non-cadaveric cell sources, such as animal cells and stem cell-derived tissues. The microcapsules that we have investigated are designed to immunoprotect islets of Langerhans (i.e. clusters of insulin-secreting cells), with the goal of treating insulin-dependent diabetes. With the aid of techniques for physicochemical analysis, this research focused on understanding which properties of the microcapsule are the most important for determining its biocompatibility. The objective of this work was to elucidate correlations between the chemical make-up, physicochemical properties, and in vivo biocompatibility of alginate-based microcapsules. Our approach was based on the hypothesis that the immune response to the microcapsules is governed by, and can therefore be controlled by, specific physicochemical properties of the microcapsule and its material components. The experimental work was divided into five phases, each associated with a specific aim : (1) To prove that immunoglobulins adsorb to the surface of alginate-polycation microcapsules, and to correlate this adsorption with the microcapsule chemistry. (2) To test interlaboratory reproducibility in making biocompatible microcapsules, and evaluate the suitability of our materials and fabrication protocols for subsequent studies. (3) To determine which physicochemical properties of alginates affect the in vivo biocompatibility of their gels. (4) To determine which physiochemical properties of alginate-polycation microcapsules are most important for determining their in vivo biocompatibility (5) To determine whether a modestly immunogenic membrane hinders or helps the ability of the microcapsule to immunoprotect islet xenografts in

  2. Radiotracer experiments with biopolymers and bio-compatible polymers

    International Nuclear Information System (INIS)

    The applications of biopolymer and biocompatible polymer employing radiotracers have been reviewed. Two different aspects have been studied. Environmentally benign methodologies for the removal, immobilization, separation or speciation of heavy, toxic elements and clinically important radionuclides have been developed using biopolymer and bio-compatible polymer as well. The complex formation ability of a bio-compatible polymer, polyvinylpyrrolidone (PVP), with clinically important radionuclides have been tested which have tremendous importance in radiopharmaceutical sciences. (author)

  3. Biocompatibility of Bletilla striata Microspheres as a Novel Embolic Agent

    OpenAIRE

    ShiHua Luo; SongLin Song; ChuanSheng Zheng; Yong Wang; XiangWen Xia; Bin Liang; GanSheng Feng

    2015-01-01

    We have prepared Chinese traditional herb Bletilla striata into microspheres as a novel embolic agent for decades. The aim of this study was to evaluate the biocompatibility of Bletilla striata microspheres (BSMs). After a thermal test of BSMs in vitro, the cell biocompatibility of BSMs was investigated in mouse fibroblasts and human umbilical vein endothelial cells using the methyl tetrazolium (MTT) assay. In addition, blood biocompatibility was evaluated. In vivo intramuscular implantation ...

  4. In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy

    Directory of Open Access Journals (Sweden)

    Dimić Ivana

    2015-01-01

    Full Text Available Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity which represents ideal environment for metallic ion release and biodegradation. The released metallic ions from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, the dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of human (MRC-5 and animal (L929 fibroblast cells viability was conducted using three in vitro test methods: colorimetric methyl-thiazol-tetrazolium (MTT test, dye exclusion test (DET and agar diffusion test (ADT. Furthermore, the cells morphology and growth were analyzed using scanning electron microscopy (SEM. The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in high concentrations that could cause detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the Co-30Cr-5Mo alloy surface. Therefore, it can be concluded that Co-30Cr-5Mo alloy is biocompatible material which can be safely used in dentistry. [Projekat Ministarstva nauke Republike Srbije, br. III 46010 i br. ON 174004

  5. Biocompatibility of surfactant-templated polyurea-nanoencapsulated macroporous silica aerogels with plasma platelets and endothelial cells.

    Science.gov (United States)

    Yin, Wei; Venkitachalam, Subramaniam M; Jarrett, Ellen; Staggs, Sarah; Leventis, Nicholas; Lu, Hongbing; Rubenstein, David A

    2010-03-15

    The recently synthesized polyurea-nanoencapsulated surfactant-templated aerogels (X-aerogels) are porous materials with significantly improved mechanical strengths. Surface-wise they resemble polyurethane, a common biocompatible material, but their biocompatibility has never been investigated. As lightweight and strong materials, if X-aerogels also have acceptable biocompatibility, they may be used in many implantable devices. The goal of this study was to investigate their biocompatibility toward platelets, blood plasma, and vascular endothelial cells, in terms of cell activation and inflammatory responses. Platelets were incubated with X-aerogel and platelet activation was measured through CD62P and phosphatidylserine expression. Platelet aggregation was also measured. Contact with X-aerogel did not induce platelet activation or impair aggregation. To determine X-aerogel-induced inflammation, plasma anaphylatoxin C3a level was measured after incubation with X-aerogel. Results showed that X-aerogel induced no changes in plasma C3a levels. SEM and SDS-PAGE were used to examine cellular/protein deposition on X-aerogel samples after plasma incubation. No structural change or organic deposition was detected. Furthermore, X-aerogel samples did not induce any significant changes in vascular endothelial cell culture parameters after 5 days of incubation. These observations suggest that X-aerogels have a suitable biocompatibility toward platelets, plasma, and vascular endothelial cells, and they have potential for use in blood implantable devices. PMID:19358258

  6. Tantalum, Niobium and Titanium Coatings for Biocompatibility Improvement of Dental Implants

    Directory of Open Access Journals (Sweden)

    Vajihesadat Mortazavi

    2007-01-01

    Full Text Available Introduction: Metals have a wide range of applications in implant and prosthetic materials in dentistry.Corrosion resistance and biocompatibility of metals should be improved in order to utilizethem as biomaterials. The aim of this work was to prepare metallic coatings on 316L stainless steel dental implants, to evaluate the corrosion characteristics of the uncoated and metallic coated dentalimplants as an indication of biocompatibility and, to compare the effect of the type of the coatings on biocompatibility.Materials and Methods: In this in vitro evaluation, three types of metallic coatings including tantalum, niobium and titanium coatings were compared using a physical vapor deposition process on 316L stainless steel dental implants. Structural characterization techniques including X-ray diffraction, scanning electron microscopy and energy dispersive X-ray analysis were utilized to investigatethe microstructure and morphology of the coatings. Electrochemical potentiodynamic tests were performed in two types of physiological solutions at 37±1°C in order to determine and compare the corrosioncurrent density and corrosion potential characteristics. The mean values were statistically compared by ANOVA at a 95% level of confidence.Results: the findings showed that all of the three types of metallic coatings had a positive effect on improvement of the corrosion behavior. The coatings could increase the corrosion resistance of 316L stainless steel and this trend was independent of the type of physiological environment.Conclusion: The biocompatible metallic coatings could decrease the corrosion current density and is a distinct advantage for prevention of ion release. Decreasing ion release can improve the biocompatibility of the dental implant, and consequently can prevent tissue damage, tissue inflammation and irritation, and can also lead to obtaining a desirable histopathological response.

  7. Synthesis of biocompatible polymers by plasma

    International Nuclear Information System (INIS)

    In this work biocompatible polymers were synthesized by plasma based on pyrrole, ethyleneglycol and allylamine. These monomers are biologically important because they contain oxygen and nitrogen in their structure and they form bonding like; N-H, C-N, C-O and O-H that are also in the human system. The polymers were synthesized with splendor electric discharges to 13.5 MHz, among 10 and 100 W, resistive coupling, pressure of 10-1 mbar and 180 minutes of reaction. The interaction of the biological systems with biomaterials depends in many cases of the properties that present the surfaces, because the rough and/or porous surfaces favor the adherence of cells. The results indicate that the ruggedness of the polymers can be controlled with the synthesis energy, since when modifying it flat and/or rough surfaces they are obtained. The compatibility of water with other solutions that it is a form of increasing the adhesion of cells with biopolymers. The affinity with water and solutions is evaluated calculating the contact angle of the polymers surface with drops of concentration solutions and similar composition to the extracellular liquid of the spinal marrow of the human body. The solutions that were proven were based on NaCl, NaCl-MgSO4, and a mixture Krebs-Ringer that has chemical composition and similar concentration to that of the fluids of the spinal marrow. In the Poly pyrrole (PPy)/Polyethyleneglycol (PEG) copolymer, the biggest angles corresponded to the Krebs-Ringer solution, in the interval of 18 to 14 degrees and those lowest to the NaCl solution, of 14.5 at 11 degrees. The Poly allylamine had the more high values with water in the interval of 16.5 to 12.5 degrees and those lowest with the NaCl solution, of 13 at 9.5 degrees. On the other hand, in the derived polymers of pyrrole the more high values corresponded to the treatment with water, until 37, and those lowest to the NaCl-MgSO4 solution, up to 10. The solutions where participated NaCl its produced the

  8. Green chemistry approach for the synthesis of biocompatible graphene

    Directory of Open Access Journals (Sweden)

    Gurunathan S

    2013-07-01

    Full Text Available Sangiliyandi Gurunathan, Jae Woong Han, Jin-Hoi Kim Department of Animal Biotechnology, Konkuk University, Seoul, South Korea Background: Graphene is a single-atom thick, two-dimensional sheet of hexagonally arranged carbon atoms isolated from its three-dimensional parent material, graphite. One of the most common methods for preparation of graphene is chemical exfoliation of graphite using powerful oxidizing agents. Generally, graphene is synthesized through deoxygenation of graphene oxide (GO by using hydrazine, which is one of the most widespread and strongest reducing agents. Due to the high toxicity of hydrazine, it is not a promising reducing agent in large-scale production of graphene; therefore, this study focused on a green or sustainable synthesis of graphene and the biocompatibility of graphene in primary mouse embryonic fibroblast cells (PMEFs. Methods: Here, we demonstrated a simple, rapid, and green chemistry approach for the synthesis of reduced GO (rGO from GO using triethylamine (TEA as a reducing agent and stabilizing agent. The obtained TEA reduced GO (TEA-rGO was characterized by ultraviolet (UV–visible absorption spectroscopy, X-ray diffraction (XRD, particle size dynamic light scattering (DLS, scanning electron microscopy (SEM, Raman spectroscopy, and atomic force microscopy (AFM. Results: The transition of graphene oxide to graphene was confirmed by UV–visible spectroscopy. XRD and SEM were used to investigate the crystallinity of graphene and the surface morphologies of prepared graphene respectively. The formation of defects further supports the functionalization of graphene as indicated in the Raman spectrum of TEA-rGO. Surface morphology and the thickness of the GO and TEA-rGO were analyzed using AFM. The presented results suggest that TEA-rGO shows significantly more biocompatibility with PMEFs cells than GO. Conclusion: This is the first report about using TEA as a reducing as well as a stabilizing agent for the

  9. Biocompatible Peritoneal Dialysis Fluids: Clinical Outcomes

    Directory of Open Access Journals (Sweden)

    Yeoungjee Cho

    2012-01-01

    Full Text Available Peritoneal dialysis (PD is a preferred home dialysis modality and has a number of added advantages including improved initial patient survival and cost effectiveness over haemodialysis. Despite these benefits, uptake of PD remains relatively low, especially in developed countries. Wider implementation of PD is compromised by higher technique failure from infections (e.g., PD peritonitis and ultrafiltration failure. These are inevitable consequences of peritoneal injury, which is thought to result primarily from continuous exposure to PD fluids that are characterised by their “unphysiologic” composition. In order to overcome these barriers, a number of more biocompatible PD fluids, with neutral pH, low glucose degradation product content, and bicarbonate buffer have been manufactured over the past two decades. Several preclinical studies have demonstrated their benefit in terms of improvement in host cell defence, peritoneal membrane integrity, and cytokine profile. This paper aims to review randomised controlled trials assessing the use of biocompatible PD fluids and their effect on clinical outcomes.

  10. Synthesis of microbial elastomers based on soybean oily acids. Biocompatibility studies

    International Nuclear Information System (INIS)

    Biocompatibility studies of the autoxidized and unoxidized unsaturated medium-long chain length (m-lcl) co-poly-3-hydroxyalkanoates (m-lclPHAs) derived from soya oily acids have been reported. Pseudomonas oleovorans was grown on a series of mixtures of octanoic acid (OA) and soya oily acids (Sy) with weight ratios of 20:80, 28:72 and 50:50 in order to obtain unsaturated m-lcl copolyesters coded PHO-Sy-2080, PHO-Sy-2872 and PHO-Sy-5050, respectively. The PHA films were obtained by solvent cast from CHCl3. They were all originally sticky and waxy except PHO-Sy-5050. Autoxidation of the unsaturated copolyester films was carried out on exposure to air at room temperature in order to obtain crosslinked polymers. They became a highly flexible elastomer after being autoxidized (about 40 days of autoxidation). The in vivo tissue reactions of the autoxidized PHAs were evaluated by subcutaneous implantation in rats. The rats appeared to be healthy throughout the implantation period. No symptom such as necrosis, abscess or tumorigenesis was observed in the vicinity of the implants. Retrieved materials varied in their physical appearance after 6 weeks of implantation. In vivo biocompatibility studies of the medical applications indicated that the microbial copolyesters obtained were all biocompatible and especially the PHOSy series of copolyesters had the highest biocompatibility among them.

  11. Nanocomposite coatings on biomedical grade stainless steel for improved corrosion resistance and biocompatibility.

    Science.gov (United States)

    Nagarajan, Srinivasan; Mohana, Marimuthu; Sudhagar, Pitchaimuthu; Raman, Vedarajan; Nishimura, Toshiyasu; Kim, Sanghyo; Kang, Yong Soo; Rajendran, Nallaiyan

    2012-10-24

    The 316 L stainless steel is one of the most commonly available commercial implant materials with a few limitations in its ease of biocompatibility and long-standing performance. Hence, porous TiO(2)/ZrO(2) nanocomposite coated over 316 L stainless steels was studied for their enhanced performance in terms of its biocompatibility and corrosion resistance, following a sol-gel process via dip-coating technique. The surface composition and porosity texture was studied to be uniform on the substrate. Biocompatibility studies on the TiO(2)/ZrO(2) nanocomposite coatings were investigated by placing the coated substrate in a simulated body fluid (SBF). The immersion procedure resulted in the complete coverage of the TiO(2)/ZrO(2) nanocomposite (coated on the surface of 316 L stainless steel) with the growth of a one-dimensional (1D) rod-like carbonate-containing apatite. The TiO(2)/ZrO(2) nanocomposite coated specimens showed a higher corrosion resistance in the SBF solution with an enhanced biocompatibility, surpassing the performance of the pure oxide coatings. The cell viability of TiO(2)/ZrO(2) nanocomposite coated implant surface was examined under human dermal fibroblasts culture, and it was observed that the composite coating enhances the proliferation through effective cellular attachment compared to pristine 316 L SS surface. PMID:22967070

  12. Designing biocompatible Ti-based metallic glasses for implant applications

    International Nuclear Information System (INIS)

    Ti-based metallic glasses show high potential for implant applications; they overcome in several crucial respects their well-established biocompatible crystalline counterparts, e.g. improved corrosion properties, higher fracture strength and wear resistance, increased elastic strain range and lower Young's modulus. However, some of the elements required for glass formation (e.g. Cu, Ni) are harmful for the human body. We critically reviewed the biological safety and glass forming tendency in Ti of 27 elements. This can be used as a basis for the future designing of novel amorphous Ti-based implant alloys entirely free of harmful additions. In this paper, two first alloys were developed: Ti75Zr10Si15 and Ti60Nb15Zr10Si15. The overheating temperature of the melt before casting can be used as the controlling parameter to produce fully amorphous materials or bcc-Ti-phase reinforced metallic glass nano-composites. The beneficial effect of Nb addition on the glass-formation and amorphous phase stability was assessed by X-ray diffraction, transmission electron microscopy and differential scanning calorimetry. Crystallization and mechanical behavior of ribbons are influenced by the amount and distribution of the nano-scaled bcc phase existing in the as-cast state. Their electrochemical stability in Ringer's solution at 310 K was found to be significantly better than that of commercial Ti-based biomaterials; no indication for pitting corrosion was recorded. Highlights: ► Link between biocompatibility and glass-forming ability of alloying additions in Ti ► Selection of Ti–Zr–Si and Ti–Zr–Nb–Si glass-forming alloys ► Two novel glassy alloys were developed: Ti75Zr10Si15 and Ti60Nb15Zr10Si15. ► Glass-formation, thermal stability, corrosion and mechanical behavior were studied. ► Assessing the suitability for orthopedic applications.

  13. Biocompatibility of Advanced Manufactured Titanium Implants—A Review

    Directory of Open Access Journals (Sweden)

    Alfred T. Sidambe

    2014-12-01

    Full Text Available Titanium (Ti and its alloys may be processed via advanced powder manufacturing routes such as additive layer manufacturing (or 3D printing or metal injection moulding. This field is receiving increased attention from various manufacturing sectors including the medical devices sector. It is possible that advanced manufacturing techniques could replace the machining or casting of metal alloys in the manufacture of devices because of associated advantages that include design flexibility, reduced processing costs, reduced waste, and the opportunity to more easily manufacture complex or custom-shaped implants. The emerging advanced manufacturing approaches of metal injection moulding and additive layer manufacturing are receiving particular attention from the implant fabrication industry because they could overcome some of the difficulties associated with traditional implant fabrication techniques such as titanium casting. Using advanced manufacturing, it is also possible to produce more complex porous structures with improved mechanical performance, potentially matching the modulus of elasticity of local bone. While the economic and engineering potential of advanced manufacturing for the manufacture of musculo-skeletal implants is therefore clear, the impact on the biocompatibility of the materials has been less investigated. In this review, the capabilities of advanced powder manufacturing routes in producing components that are suitable for biomedical implant applications are assessed with emphasis placed on surface finishes and porous structures. Given that biocompatibility and host bone response are critical determinants of clinical performance, published studies of in vitro and in vivo research have been considered carefully. The review concludes with a future outlook on advanced Ti production for biomedical implants using powder metallurgy.

  14. Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films

    International Nuclear Information System (INIS)

    In previous work, we developed novel antibacterial hybrid coatings based on dextran containing dispersed Ag NPs (∼ 5 nm, DEX-Ag) aimed to offer dual protection against two of the most common complications associated with implant surgery, infections and rejection of the implant. However, their blood-material interactions are unknown. In this study, we assess the hemocompatibility and biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-inflammatory eicosanoid expression (Prostaglandin E2, PGE2) and release of reactive oxygen species (ROS, superoxide and H2O2) following incubation of the cells with the surfaces. The morphology and cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS, the expression of pro-inflammatory cytokines remained minimal with similar levels of PGE2, as compared to the other surfaces studied. These results highlight low toxicity of DEX-Ag and hold promise for future applications in vivo. - Highlights: • We examined specific blood-contact reactions of dextran doped with Ag NPs coatings. • Biocompatibility was assessed with THP-1 cells and PMA-stimulated THP-1 cells. • Glass, polyurethane and dextran were used as reference surfaces. • Hybrid coatings exhibited non-hemolytic properties. • Low toxicity, inflammatory response and ROS suggest potential for in vivo use

  15. Composite material

    Energy Technology Data Exchange (ETDEWEB)

    Hutchens, Stacy A. (Knoxville, TN); Woodward, Jonathan (Solihull, GB); Evans, Barbara R. (Oak Ridge, TN); O' Neill, Hugh M. (Knoxville, TN)

    2012-02-07

    A composite biocompatible hydrogel material includes a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa. A calcium comprising salt is disposed in at least some of the pores. The porous polymer matrix can comprise cellulose, including bacterial cellulose. The composite can be used as a bone graft material. A method of tissue repair within the body of animals includes the steps of providing a composite biocompatible hydrogel material including a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa, and inserting the hydrogel material into cartilage or bone tissue of an animal, wherein the hydrogel material supports cell colonization in vitro for autologous cell seeding.

  16. Composite material

    Science.gov (United States)

    Hutchens, Stacy A.; Woodward, Jonathan; Evans, Barbara R.; O'Neill, Hugh M.

    2012-02-07

    A composite biocompatible hydrogel material includes a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa. A calcium comprising salt is disposed in at least some of the pores. The porous polymer matrix can comprise cellulose, including bacterial cellulose. The composite can be used as a bone graft material. A method of tissue repair within the body of animals includes the steps of providing a composite biocompatible hydrogel material including a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa, and inserting the hydrogel material into cartilage or bone tissue of an animal, wherein the hydrogel material supports cell colonization in vitro for autologous cell seeding.

  17. Coating with Autologous Plasma Improves Biocompatibility of Mesh Grafts In Vitro: Development Stage of a Surgical Innovation

    Directory of Open Access Journals (Sweden)

    Holger Gerullis

    2013-01-01

    Full Text Available Purpose. To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials. Materials and Methods. Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs, platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue assay, representative for fibroblasts, muscle cells, and endothelial cells originating from 10 different patients. Adherence of those tissues on the meshes was microscopically investigated and semiquantitatively assessed using a previously described scoring system. Results. Coating with peripheral blood mononuclear cells did not affect the adherence score, whereas coating with platelets and blood plasma increased the score suggesting improved biocompatibility in vitro. The previous ranking of native meshes remained consistent after coating. Conclusion. Plasma coating of meshes improves their biocompatibility score in a novel in vitro test system.

  18. Peptide-functionalized hydrogels for enhancing biocompatibility of encapsulating materials

    Czech Academy of Sciences Publication Activity Database

    Mihálová, A.; Lahová, M.; Bekešová, S.; Škultéty, L.; Proks, Vladimír; Kučka, Jan; Lacík, I.

    Prague : Institute of Macromolecular Chemistry AS CR, 2012. s. 101. ISBN 978-80-85009-72-9. [Prague Meeting on Macromolecules /76./ - Polymers in Medicine. 01.07.2012-05.07.2012, Prague] Institutional support: RVO:61389013 Keywords : hydrogels * monocyte chemotactic protein Subject RIV: CD - Macromolecular Chemistry

  19. Determination of Transport Characteristics of Porous Biocompatible Materials

    Czech Academy of Sciences Publication Activity Database

    Soukup, Karel; Hejtmánek, Vladimír; Šolcová, Olga

    Athens : WSEAS Press, 2013 - (Moller, J.; Kibler, M.; Hefferlin, R.), s. 66-71 ISBN 978-960-474-342-1 R&D Projects: GA ČR GPP106/11/P459; GA ČR(CZ) GAP204/11/1206 Institutional support: RVO:67985858 Keywords : transport characteristics * biocompatiblematerials * Wicke-Kallenbach cell Subject RIV: CI - Industrial Chemistry, Chemical Engineering http://www.wseas.org/cms.action?id=5628

  20. In vitro calcification and in vivo biocompatibility of the cross-linked polypentapeptide of elastin

    International Nuclear Information System (INIS)

    The in vitro calcifiability and molecular weight dependence of calcification of the polypentapeptide, (L X Val1-L X Pro2-Gly3-L X Val4-Gly5)n, which had been gamma-irradiation cross-linked have been determined when exposed to dialyzates of normal, nonaugmented fetal bovine serum. The material was found to calcify: calcifiability was found to be highly molecular weight dependent and to be most favored when the highest molecular weight polymers (n approximately equal to 240) had been used for cross-linking. The in vivo biocompatibility, biodegradability, and calcifiability of the gamma-irradiation cross-linked polypentapeptide were examined in rabbits in both soft and hard tissue sites. The material was found to be biocompatible irrespective of its physical form and to be biodegradable but with n of 200 or less it was not shown to calcify or ossify in the rabbit tibial nonunion model

  1. Boron-Doped Nanocrystalline Diamond Electrodes for Neural Interfaces: In vivo Biocompatibility Evaluation.

    Science.gov (United States)

    Alcaide, María; Taylor, Andrew; Fjorback, Morten; Zachar, Vladimir; Pennisi, Cristian P

    2016-01-01

    Boron-doped nanocrystalline diamond (BDD) electrodes have recently attracted attention as materials for neural electrodes due to their superior physical and electrochemical properties, however their biocompatibility remains largely unexplored. In this work, we aim to investigate the in vivo biocompatibility of BDD electrodes in relation to conventional titanium nitride (TiN) electrodes using a rat subcutaneous implantation model. High quality BDD films were synthesized on electrodes intended for use as an implantable neurostimulation device. After implantation for 2 and 4 weeks, tissue sections adjacent to the electrodes were obtained for histological analysis. Both types of implants were contained in a thin fibrous encapsulation layer, the thickness of which decreased with time. Although the level of neovascularization around the implants was similar, BDD electrodes elicited significantly thinner fibrous capsules and a milder inflammatory reaction at both time points. These results suggest that BDD films may constitute an appropriate material to support stable performance of implantable neural electrodes over time. PMID:27013949

  2. Boron-doped nanocrystalline diamond electrodes for neural interfaces: In vivo biocompatibility evaluation

    Directory of Open Access Journals (Sweden)

    María eAlcaide

    2016-03-01

    Full Text Available Boron-doped nanocrystalline diamond (BDD electrodes have recently attracted attention as materials for neural electrodes due to their superior physical and electrochemical properties, however their biocompatibility remains largely unexplored. In this work, we aim to investigate the in vivo biocompatibility of BDD electrodes in relation to conventional titanium nitride (TiN electrodes using a rat subcutaneous implantation model. High quality BDD films were synthesized on electrodes intended for use as an implantable neurostimulation device. After implantation for 2 and 4 weeks, tissue sections adjacent to the electrodes were obtained for histological analysis. Both types of implants were contained in a thin fibrous encapsulation layer, the thickness of which decreased with time. Although the level of neovascularization around the implants was similar, BDD electrodes elicited significantly thinner fibrous capsules and a milder inflammatory reaction at both time points. These results suggest that BDD films may constitute an appropriate material to support stable performance of implantable neural electrodes over time.

  3. Boron-Doped Nanocrystalline Diamond Electrodes for Neural Interfaces: In vivo Biocompatibility Evaluation

    Science.gov (United States)

    Alcaide, María; Taylor, Andrew; Fjorback, Morten; Zachar, Vladimir; Pennisi, Cristian P.

    2016-01-01

    Boron-doped nanocrystalline diamond (BDD) electrodes have recently attracted attention as materials for neural electrodes due to their superior physical and electrochemical properties, however their biocompatibility remains largely unexplored. In this work, we aim to investigate the in vivo biocompatibility of BDD electrodes in relation to conventional titanium nitride (TiN) electrodes using a rat subcutaneous implantation model. High quality BDD films were synthesized on electrodes intended for use as an implantable neurostimulation device. After implantation for 2 and 4 weeks, tissue sections adjacent to the electrodes were obtained for histological analysis. Both types of implants were contained in a thin fibrous encapsulation layer, the thickness of which decreased with time. Although the level of neovascularization around the implants was similar, BDD electrodes elicited significantly thinner fibrous capsules and a milder inflammatory reaction at both time points. These results suggest that BDD films may constitute an appropriate material to support stable performance of implantable neural electrodes over time. PMID:27013949

  4. In-situ biopreparation of biocompatible bacterial cellulose/graphene oxide composites pellets

    Science.gov (United States)

    Zhu, Wenkun; Li, Wei; He, Yi; Duan, Tao

    2015-05-01

    In the present work, a composite material formed by bacterial cellulose (BC) and graphene oxide (GO) was synthesized and characterized. GO was added in the bacteria culture media and then the bacteria was inoculated. The BC/GO pellets composite was prepared into the bacteria culture media and vibrated in Erlenmeyer flask. Characterization of the BC/GO composite showed GO nanosheets embedded in the nanofibers network of BC. The experiments in this study demonstrated BC and GO effectively interacted by hydrogen bonding. Moreover, the cytotoxicity assays showed the material had a better biocompatibility than the counterparts and promoted the cell proliferation excellently. The biocompatibility of BC/GO has the potential to be used for drug delivery.

  5. Viability of biocompatible and biodegradable seeds production with incorporated radionuclides

    International Nuclear Information System (INIS)

    The present work aims the development of radioactive seeds, biocompatible and biodegradable, with the objective of adding options in the cancer treatment. The work focus on the production of seeds biodegradable that incorporate radioisotopes with half life inferior than the degradation time of the material. The idea of producing devices with biodegradable materials impregnated with radioisotopes of short half life will offer new possibilities in the cancer treatment, since they can be used following the same procedures of the permanent interstitial brachytherapy, but using degradable materials compatible with the physiological environment. It will be discussed in particular the possible application of these seeds in the treatment of prostate cancer. A review of the subject and a preliminary evaluation of the viability of production of the seeds will be presented. The method of production of the seeds is based on the incorporation of Iodine and Samarium in glass matrixes obtained by sol-gel processing. X-ray fluorescence was done in the samples produced and the incorporation of Iodine and Samarium atoms was confirmed. (author)

  6. Biomedical-Grade Chitosan in Wound Management and Its Biocompatibility In Vitro

    OpenAIRE

    Halim, Ahmad Sukari; Lim, Chin Keong

    2010-01-01

    Chitosan and chitosan-based derivatives have various medical applications. It is wellknown that chitosan possesses medicinal properties that accelerate wound healing and tissue regeneration. Chitosan is a natural product. It is biocompatible and biodegradable, enabling it to be used for wound dressing material. However, the practical use of chitosan is restricted to the unmodified forms, as these are water-insoluble and have high viscosity and the tendency to coagulate with proteins at high p...

  7. Blends of Thermoplastic Polyurethane and Polydimethylsiloxane Rubber: Assessment of Biocompatibility and Suture Holding Strength of Membranes

    OpenAIRE

    Krishna Prasad Rajan; Ahmed Al-Ghamdi; Ramesh Parameswar; Nando, G. B.

    2013-01-01

    In the present investigation, a compatibilized blend of thermoplastic polyurethane (TPU) and polydimethylsiloxane (PDMS) is prepared by using copolymer of ethylene and methyl acrylate (EMA) as a reactive compatibilizer. Detailed in vitro biocompatibility studies were carried out for this compatibilized blend and the material was found noncytotoxic towards L929 mouse fibroblast subcutaneous connective tissue cell line. Microporosity was created on the surface of membranes prepared from the ble...

  8. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton's Jelly Mesenchymal Stem Cells

    OpenAIRE

    Kewalin Inthanon; Donraporn Daranarong; Pimwalan Techaikool; Winita Punyodom; Vorathep Khaniyao; Audrey M Bernstein; Weerah Wongkham

    2015-01-01

    Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited q...

  9. Novel biocompatible nanocapsules for slow release of fragrances on the human skin

    OpenAIRE

    Hosseinkhani, Baharak; Callewaert, Chris; Vanbeveren, Nelleke; Boon, Nico

    2015-01-01

    There is a growing demand for fragranced products, but due to the poor aqueous solubility and instability of fragrance molecules, their use is limited. Nowadays, fragrance encapsulation in biocompatible nanocontainer material is emerging as a novel strategy to overcome the evaporation of volatile molecules and to prolong the sensory characteristics of fragrance molecules and the longevity of perfumes. The objective of this study was to develop an innovative sustained release system of perfume...

  10. Rational Design and Enhanced Biocompatibility of a Dry Adhesive Medical Skin Patch

    KAUST Repository

    Kwak, Moon Kyu

    2011-07-28

    A new type of medical skin patch is developed that contains high-density, mushroom-like micropillars. Such dry-adhesive micropillars are highly biocompatible, have minimized side effects, and provide reasonable normal adhesion strength. To arrive at optimal conditions for the dry adhesive skin patch, the proper design of various structural and material parameters of micropillars is investigated. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Development of a curved, stratified, in vitro model to assess ocular biocompatibility.

    Directory of Open Access Journals (Sweden)

    Cameron K Postnikoff

    Full Text Available PURPOSE: To further improve in vitro models of the cornea, this study focused on the creation of a three-dimensional, stratified, curved epithelium; and the subsequent characterization and evaluation of its suitability as a model for biocompatibility testing. METHODS: Immortalized human corneal epithelial cells were grown to confluency on curved cellulose filters for seven days, and were then differentiated and stratified using an air-liquid interface for seven days before testing. Varying concentrations of a commercial ophthalmic solution containing benzalkonium chloride (BAK, a known cytotoxic agent, and two relevant ocular surfactants were tested on the model. A whole balafilcon A lens soaked in phosphate buffered saline (BA PBS was also used to assess biocompatibility and verify the validity of the model. Viability assays as well as flow cytometry were performed on the cells to investigate changes in cell death and integrin expression. RESULTS: The reconstructed curved corneal epithelium was composed of 3-5 layers of cells. Increasing concentrations of BAK showed dose-dependent decreased cell viability and increased integrin expression and cell death. No significant change in viability was observed in the presence of the surfactants. As expected, the BA PBS combination appeared to be very biocompatible with no adverse change in cell viability or integrin expression. CONCLUSIONS: The stratified, curved, epithelial model proved to be sensitive to distinct changes in cytotoxicity and is suitable for continued assessment for biocompatibility testing of contact lenses. Our results showed that flow cytometry can provide a quantitative measure of the cell response to biomaterials or cytotoxic compounds for both the supernatant and adherent cell populations. As a specifically designed in vitro model of the corneal epithelium, this quantitative model for biocompatibility at the ocular surface may help improve our understanding of cell-material

  12. Initial biocompatibility of plasma polymerized hexamethyldisiloxane films with different wettability

    Energy Technology Data Exchange (ETDEWEB)

    Krasteva, N A; Toromanov, G; Hristova, K T; Radeva, E I; Pecheva, E V; Dimitrova, R P; Altankov, G P; Pramatarova, L D, E-mail: nataly@bio21.bas.b

    2010-11-01

    Understanding the relationships between material surface properties, behaviour of adsorbed proteins and cellular responses is essential to design optimal material surfaces for tissue engineering. In this study we modify thin layers of plasma polymerized hexamethyldisiloxane (PPHMDS) by ammonia treatment in order to increase surface wettability and the corresponding biological response. The physico-chemical properties of the polymer films were characterized by contact angle (CA) measurements and Fourier Transform Infrared Spectroscopy (FTIR) analysis.Human umbilical vein endothelial cells (HUVEC) were used as model system for the initial biocompatibility studies following their behavior upon preadsorption of polymer films with three adhesive proteins: fibronectin (FN), fibrinogen (FG) and vitronectin (VN). Adhesive interaction of HUVEC was evaluated after 2 hours by analyzing the overall cell morphology, and the organization of focal adhesion contacts and actin cytoskeleton. We have found similar good cellular response on FN and FG coated polymer films, with better pronounced vinculin expression on FN samples while. Conversely, on VN coated surfaces the wettability influenced significantly initial celular interaction spreading. The results obtained suggested that ammonia plasma treatment can modulate the biological activity of the adsorbed protein s on PPHMDS surfaces and thus to influence the interaction with endothelial cells.

  13. Effect of plasma surface modification on the biocompatibility of UHMWPE

    Energy Technology Data Exchange (ETDEWEB)

    Kaklamani, G; Chen, J; Dong, H; Stamboulis, A [School of Metallurgy and Materials, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom); Mehrban, N; Bowen, J; Grover, L, E-mail: a.stamboulis@bham.ac.u [School of Chemical Engineering, College of Engineering and Physical Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom)

    2010-10-01

    In this paper active screen plasma nitriding (ASPN) is used to chemically modify the surface of UHMWPE. This is an unexplored and new area of research. ASPN allows the homogeneous treatment of any shape or surface at low temperature; therefore, it was thought that ASPN would be an effective technique to modify organic polymer surfaces. ASPN experiments were carried out at 120 {sup 0}C using a dc plasma nitriding unit with a 25% N{sub 2} and 75% H{sub 2} atmosphere at 2.5 mbar of pressure. UHMWPE samples treated for different time periods were characterized by nanoindentation, FTIR, XPS, interferometry and SEM. A 3T3 fibroblast cell line was used for in vitro cell culture experiments. Nanoindentation of UHMWPE showed that hardness and elastic modulus increased with ASPN treatment compared to the untreated material. FTIR spectra did not show significant differences between the untreated and treated samples; however, some changes were observed at 30 min of treatment in the range of 1500-1700 cm{sup -1} associated mainly with the presence of N-H groups. XPS studies showed that nitrogen was present on the surface and its amount increased with treatment time. Interferometry showed that no significant changes were observed on the surfaces after the treatment. Finally, cell culture experiments and SEM showed that fibroblasts attached and proliferated to a greater extent on the plasma-treated surfaces leading to the conclusion that ASPN surface treatment can potentially significantly improve the biocompatibility behaviour of polymeric materials.

  14. Metallic ion release from biocompatible cobalt-based alloy

    Directory of Open Access Journals (Sweden)

    Dimić Ivana D.

    2014-01-01

    Full Text Available Metallic biomaterials, which are mainly used for the damaged hard tissue replacements, are materials with high strength, excellent toughness and good wear resistance. The disadvantages of metals as implant materials are their susceptibility to corrosion, the elastic modulus mismatch between metals and human hard tissues, relatively high density and metallic ion release which can cause serious health problems. The aim of this study was to examine metallic ion release from Co-Cr-Mo alloy in artificial saliva. In that purpose, alloy samples were immersed into artificial saliva with different pH values (4.0, 5.5 and 7.5. After a certain immersion period (1, 3 and 6 weeks the concentrations of released ions were determined using Inductively Coupled Plasma - Mass Spectrophotometer (ICP-MS. The research findings were used in order to define the dependence between the concentration of released metallic ions, artificial saliva pH values and immersion time. The determined released metallic ions concentrations were compared with literature data in order to describe and better understand the phenomenon of metallic ion release from the biocompatible cobalt-based alloy. [Projekat Ministarstva nauke Republike Srbije, br. III 46010 i br. ON 174004

  15. Effect of plasma surface modification on the biocompatibility of UHMWPE.

    Science.gov (United States)

    Kaklamani, G; Mehrban, N; Chen, J; Bowen, J; Dong, H; Grover, L; Stamboulis, A

    2010-10-01

    In this paper active screen plasma nitriding (ASPN) is used to chemically modify the surface of UHMWPE. This is an unexplored and new area of research. ASPN allows the homogeneous treatment of any shape or surface at low temperature; therefore, it was thought that ASPN would be an effective technique to modify organic polymer surfaces. ASPN experiments were carried out at 120 °C using a dc plasma nitriding unit with a 25% N(2) and 75% H(2) atmosphere at 2.5 mbar of pressure. UHMWPE samples treated for different time periods were characterized by nanoindentation, FTIR, XPS, interferometry and SEM. A 3T3 fibroblast cell line was used for in vitro cell culture experiments. Nanoindentation of UHMWPE showed that hardness and elastic modulus increased with ASPN treatment compared to the untreated material. FTIR spectra did not show significant differences between the untreated and treated samples; however, some changes were observed at 30 min of treatment in the range of 1500-1700 cm(-1) associated mainly with the presence of N-H groups. XPS studies showed that nitrogen was present on the surface and its amount increased with treatment time. Interferometry showed that no significant changes were observed on the surfaces after the treatment. Finally, cell culture experiments and SEM showed that fibroblasts attached and proliferated to a greater extent on the plasma-treated surfaces leading to the conclusion that ASPN surface treatment can potentially significantly improve the biocompatibility behaviour of polymeric materials. PMID:20876959

  16. Fabrication and Biocompatibility of Electrospun Silk Biocomposites

    Directory of Open Access Journals (Sweden)

    Ick-Soo Kim

    2011-10-01

    Full Text Available Silk fibroin has attracted great interest in tissue engineering because of its outstanding biocompatibility, biodegradability and minimal inflammatory reaction. In this study, two kinds of biocomposites based on regenerated silk fibroin are fabricated by electrospinning and post-treatment processes, respectively. Firstly, regenerated silk fibroin/tetramethoxysilane (TMOS hybrid nanofibers with high hydrophilicity are prepared, which is superior for fibroblast attachment. The electrospinning process causes adjacent fibers to ‘weld’ at contact points, which can be proved by scanning electron microscope (SEM. The water contact angle of silk/tetramethoxysilane (TMOS composites shows a sharper decrease than pure regenerated silk fibroin nanofiber, which has a great effect on the early stage of cell attachment behavior. Secondly, a novel tissue engineering scaffold material based on electrospun silk fibroin/nano-hydroxyapatite (nHA biocomposites is prepared by means of an effective calcium and phosphate (Ca–P alternate soaking method. nHA is successfully produced on regenerated silk fibroin nanofiber within several min without any pre-treatments. The osteoblastic activities of this novel nanofibrous biocomposites are also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP activity is ameliorated on mineralized silk nanofibers. All these results indicate that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering.

  17. Effect of plasma surface modification on the biocompatibility of UHMWPE

    International Nuclear Information System (INIS)

    In this paper active screen plasma nitriding (ASPN) is used to chemically modify the surface of UHMWPE. This is an unexplored and new area of research. ASPN allows the homogeneous treatment of any shape or surface at low temperature; therefore, it was thought that ASPN would be an effective technique to modify organic polymer surfaces. ASPN experiments were carried out at 120 0C using a dc plasma nitriding unit with a 25% N2 and 75% H2 atmosphere at 2.5 mbar of pressure. UHMWPE samples treated for different time periods were characterized by nanoindentation, FTIR, XPS, interferometry and SEM. A 3T3 fibroblast cell line was used for in vitro cell culture experiments. Nanoindentation of UHMWPE showed that hardness and elastic modulus increased with ASPN treatment compared to the untreated material. FTIR spectra did not show significant differences between the untreated and treated samples; however, some changes were observed at 30 min of treatment in the range of 1500-1700 cm-1 associated mainly with the presence of N-H groups. XPS studies showed that nitrogen was present on the surface and its amount increased with treatment time. Interferometry showed that no significant changes were observed on the surfaces after the treatment. Finally, cell culture experiments and SEM showed that fibroblasts attached and proliferated to a greater extent on the plasma-treated surfaces leading to the conclusion that ASPN surface treatment can potentially significantly improve the biocompatibility behaviour of polymeric materials.

  18. Biocompatibility evaluation in vitro. Part II: Functional expression of human and animal osteoblasts on the biomaterials

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    DNA synthesis and collagen formations on the implant material by cell culture in vitro are the most important phenotypical expression to estimate the biocompatibility. In this part, DNA synthesis and collagen formation on implant materials were quantitatively and qualitatively estimated by radioactive isotope H+-thymidine to incorporate into DNA chains, H+-proline to incorporate into type I collagen proteins followed by scin-tillation counting and antibody-antigen immunocytochemistry staining, respectively. Research results demonstrate that hydroxyapatite (HA) stimulates DNA synthesis and collagen formation on the material whereas this stimulation is restricted by adding spinel to the materials. There are statistical differences between the influences of material components on both DNA synthesis and collagen formation. It is supposed that porous materials can supply more platforms for cell anchoring, and more DNA and collagen are synthesised on the porous materials. Immersion in culture medium results in new HA crystal formation on the porous HA materials.

  19. Towards a biocompatible artificial lung: Covalent functionalization of poly(4-methylpent-1-ene (TPX with cRGD pentapeptide

    Directory of Open Access Journals (Sweden)

    Lena Möller

    2013-02-01

    Full Text Available Covalent multistep coating of poly(methylpentene, the membrane material in lung ventilators, by using a copper-free “click” approach with a modified cyclic RGD peptide, leads to a highly biocompatible poly(methylpentene surface. The resulting modified membrane preserves the required excellent gas-flow properties while being densely seeded with lung endothelial cells.

  20. Síntese de hidrogéis biocompatíveis para encapsulamento de fármacos

    OpenAIRE

    Monteiro, Mariana Marinho Caires

    2014-01-01

    Este projecto tem como tema a Síntese de hidrogéis biocompatíveis para encapsulamento de fármacos e como objectivo produzir um material biocompatível, mais propriamente um hidrogel biopolimérico, com características específicas capazes de promover o encapsulamento do fármaco 5-fluorouracilo (5-Fu). Os materiais biopoliméricos são bastante atraentes para aplicações biomédicas, uma vez que podem combinar a degradabilidade in vivo com a boa biocompatibilidade e, desta forma, po...

  1. An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones

    Directory of Open Access Journals (Sweden)

    Boos Alois

    2006-08-01

    Full Text Available Abstract Background The past years have seen the development of many synthetic bone replacements. To test their biocompatibility and ability for osseointegration, osseoinduction and -conduction requires their placement within bone preferably in an animal experiment of a higher species. Methods A suitable experimental animal model in sheep with drill holes of 8 mm diameter and 13 mm depth within the proximal and distal humerus and femur for testing biocompatibility issues is introduced. Results This present sheep model allows the placing of up to 8 different test materials within one animal and because of the standardization of the bone defect, routine evaluation by means of histomorphometry is easily conducted. This method was used successfully in 66 White Alpine Sheep. When the drill holes were correctly placed no complications such as spontaneous fractures were encountered. Conclusion This experimental animal model serves an excellent basis for testing the biocompatibility of novel biomaterials to be used as bone replacement or new bone formation enhancing materials.

  2. Influence of surface modification on corrosion and biocompatibility of titanium alloys

    Science.gov (United States)

    Rahman, Zia Ur

    Titanium alloys are playing a vital role in the field of biomaterials due to their excellent corrosion resistance and biocompatibility. These alloys enhance the quality and longevity of human life by replacing or treating various parts of the body. However, as these materials are in constant contact with the aggressive body fluids, corrosion leads to metal ions dissolution. These ions leach to the adjacent tissues and causes adverse reactions. Surface modifications are used to improve corrosion resistance and biological activity without changing their bulk properties. In this investigation, electropolishing, magnetoelectropolishing, titanium coating and hydroxiapatitecoating were carried out on commercially pure titanium (CPTi), Ti6Al4V and Ti6Al4V-ELI (Extra Low Interstitials). These surface modifications are known to effect surface charge, chemistry, morphology; wettability, corrosion resistance and biocompatibility of these materials. In vitro cyclic potentiodynamic polarization tests were conducted in phosphate buffer saline in compliance with ASTM standard. The surface morphology, roughness and wettability of these alloys were studied using scanning electron microscope, atomic force microscope and contact angle meter, respectively. Moreover, biocompatibility of titanium alloys was assessed by growing MC3T3 pre-osteoblast cells on their surfaces

  3. Mechanical Strength and Biocompatibility of Ultrafine-Grained Commercial Purity Titanium

    Directory of Open Access Journals (Sweden)

    Yuri Estrin

    2013-01-01

    Full Text Available The effect of grain refinement of commercial purity titanium by equal channel angular pressing (ECAP on its mechanical performance and bone tissue regeneration is reported. In vivo studies conducted on New Zealand white rabbits did not show an enhancement of biocompatibility of ECAP-modified titanium found earlier by in vitro testing. However, the observed combination of outstanding mechanical properties achieved by ECAP without a loss of biocompatibility suggests that this is a very promising processing route to bioimplant manufacturing. The study thus supports the expectation that commercial purity titanium modified by ECAP can be seen as an excellent candidate material for bone implants suitable for replacing conventional titanium alloy implants.

  4. Biocompatibility and preliminary clinical application of HA/HDPE nanocomposites synthetic auditory ossicle

    Institute of Scientific and Technical Information of China (English)

    ZHU Shai-hong; WANG Guo-hui; ZHAO Yan-zhong; QI You-fei; ZHOU Ke-chao; HUANG Su-ping; LI Zhi-you; HUANG Bai-yun

    2006-01-01

    The biocompatibility of the hydroxyapatite/high density polyethtlene(HA/HDPE) nanocomposites synthetic auditory ossicle was evaluated, the percentage of S-period cells was detected by flow cytometry after L929 incubated with extraction of the HA/HDPE nanocomposites, titanium materials of clinical application as the control. Both of them were implanted in the animals and the histopathological evaluations were carried out, and the preliminary clinical trials about HA/HDPE nanocomposites synthetic auditory ossicles were also carried out. The statistical analysis show that there are no statistically significant differences between HA/HDPE test groups and control groups (P>0.05), which demonstrates that the HA/HDPE nanocomposites synthetic auditory ossicle has a good biocompatibility and clinical application outlook.

  5. Wettability and biocompatibility of nitrogen-doped hydrogenated amorphous carbon films: Effect of nitrogen

    International Nuclear Information System (INIS)

    Amorphous carbon films have been applied in biomedical fields as potential biocompatible materials with wettability that can be adjusted by doping with other elements, including F, Si, Ti, O and N. In this study, nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) films were deposited by PIII-D using C2H2 + N2 gas mixtures. The biocompatibility and anti-thrombotic properties of the films were assessed in vitro. The surface morphology and surface wettability of the films were characterized using atomic force microscopy (AFM) and a contact angle method. The results show no cytotoxicity for all films, and films with appropriate nitrogen doping possess much better endothelial cell growth and anti-thrombotic properties

  6. MeV ion beam lithography of biocompatible halogenated Parylenes using aperture masks

    Science.gov (United States)

    Whitlow, Harry J.; Norarat, Rattanaporn; Roccio, Marta; Jeanneret, Patrick; Guibert, Edouard; Bergamin, Maxime; Fiorucci, Gianni; Homsy, Alexandra; Laux, Edith; Keppner, Herbert; Senn, Pascal

    2015-07-01

    Parylenes are poly(p-xylylene) polymers that are widely used as moisture barriers and in biomedicine because of their good biocompatibility. We have investigated MeV ion beam lithography using 16O+ ions for writing defined patterns in Parylene-C, which is evaluated as a coating material for the Cochlear Implant (CI) electrode array, a neuroprosthesis to treat some forms of deafness. Parylene-C and -F on silicon and glass substrates as well as 50 μm thick PTFE were irradiated to different fluences (1 ×1013 - 1 ×1016 1 MeV 16O+ ions cm-2) through aperture masks under high vacuum and a low pressure (PTFE but with a ∼25× smaller removal rate. Biocompatibility tests showed no difference in cell adhesion between irradiated and unirradiated areas or ion fluence dependence. Coating the Parylene surface with an adhesion-promoting protein mixture had a much greater effect on cell proliferation.

  7. Biocompatible Single-Crystal Selenium Nanobelt Based Nanodevice as a Temperature-Tunable Photosensor

    Directory of Open Access Journals (Sweden)

    Yongshan Niu

    2012-01-01

    Full Text Available Selenium materials are widely used in photoelectrical devices, owing to their unique semiconductive properties. Single-crystal selenium nanobelts with large specific surface area, fine photoconductivity, and biocompatibility provide potential applications in biomedical nanodevices, such as implantable artificial retina and rapid photon detector/stimulator for optogenetics. Here, we present a selenium nanobelt based nanodevice, which is fabricated with single Se nanobelt. This device shows a rapid photo response, different sensitivities to visible light of variable wave length, and temperature-tunable property. The biocompatibility of the Se nanobelts was proved by MTT test using two cell lines. Our investigation introduced a photosensor that will be important for multiple potential applications in human visual system, photocells in energy or MEMS, and temperature-tunable photoelectrical device for optogenetics research.

  8. Building Biocompatible Hydrogels for Tissue Engineering of the Brain and Spinal Cord

    Directory of Open Access Journals (Sweden)

    Kimberly B. Bjugstad

    2012-11-01

    Full Text Available Tissue engineering strategies employing biomaterials have made great progress in the last few decades. However, the tissues of the brain and spinal cord pose unique challenges due to a separate immune system and their nature as soft tissue. Because of this, neural tissue engineering for the brain and spinal cord may require re-establishing biocompatibility and functionality of biomaterials that have previously been successful for tissue engineering in the body. The goal of this review is to briefly describe the distinctive properties of the central nervous system, specifically the neuroimmune response, and to describe the factors which contribute to building polymer hydrogels compatible with this tissue. These factors include polymer chemistry, polymerization and degradation, and the physical and mechanical properties of the hydrogel. By understanding the necessities in making hydrogels biocompatible with tissue of the brain and spinal cord, tissue engineers can then functionalize these materials for repairing and replacing tissue in the central nervous system.

  9. Mesoporous and biocompatible surface active silica aerogel synthesis using choline formate ionic liquid.

    Science.gov (United States)

    Meera, Kamal Mohamed Seeni; Sankar, Rajavelu Murali; Jaisankar, Sellamuthu N; Mandal, Asit Baran

    2011-09-01

    In this paper, we report the preparation and characterization of mesoporous and biocompatible transparent silica aerogel by the sol-gel polymerization of tetraethyl orthosilicate using ionic liquid. Choline cation based ionic liquid allows the silica framework to form in a non collapsing environment and controls the pore size of the gel. FT-IR spectra reveal the interaction of ionic liquid with surface -OH of the gel. DSC thermogram giving the evidence of confinement of ionic liquid within the silica matrix, which helps to avoid the shrinkage of the gel during the aging process. Nitrogen sorption measurements of gel prepared with ionic liquid exhibit a low surface area of 100.53 m2/g and high average pore size of 3.74 nm. MTT assay proves the biocompatibility and cell viability of the prepared gels. This new nanoporous silica material can be applied to immobilize biological molecules, which may retain their stability over a longer period. PMID:21565470

  10. Overview of Stabilizing Ligands for Biocompatible Quantum Dot Nanocrystals

    Directory of Open Access Journals (Sweden)

    Aaron Clapp

    2011-11-01

    Full Text Available Luminescent colloidal quantum dots (QDs possess numerous advantages as fluorophores in biological applications. However, a principal challenge is how to retain the desirable optical properties of quantum dots in aqueous media while maintaining biocompatibility. Because QD photophysical properties are directly related to surface states, it is critical to control the surface chemistry that renders QDs biocompatible while maintaining electronic passivation. For more than a decade, investigators have used diverse strategies for altering the QD surface. This review summarizes the most successful approaches for preparing biocompatible QDs using various chemical ligands.

  11. In vivo biocompatibility of Resilon compared with gutta-percha in a pre-clinical model

    Directory of Open Access Journals (Sweden)

    Miguel Cardoso

    2013-01-01

    Full Text Available Background: The aim of this study was to investigate in vivo biocompatibility of Resilon, compared with gutta-percha, at short and long-term following implantation in a rat subcutaneous implantation model. Materials and Methods: Male Wistar rats were implanted subcutaneously with either Resilon or gutta-percha or were sham controls. Tissues were harvested at 8 days or 60 days after implantation and were evaluated histologically for inflammation and fibrous encapsulation. The severity of histologic injury, scored on a scale of 0-4 and quantitative analysis of the capsule wall thickness were determined for statistical analysis. Data were analyzed by Student t-test, one-way analysis of variance, Kruskal-Wallis or Mann-Whitney′s tests as appropriate. A value of P ≤ 0.05 was considered statistically significant. Results: No behavioral changes or visible signs of physical impairment were observed at 8 days or 60 days post-implantation. Histopathologic observation of the implanted sites at each time-point showed that both Resilon and gutta-percha implants induced foreign body reaction, showing minimal to mild inflammatory reactions in most cases, which diminished significantly with time. Compared with gutta-percha, the capsule wall was thinner (P > 0.05 after Resilon implantation at day 8 and significantly (P = 0.01 thicker at day 60. In addition, capsule wall thickness showed a trend to increase with time after implantation in the Resilon groups (P > 0.05, opposed to the significant decrease (P = 0.016 observed after implantation in the gutta-percha groups, suggesting lesser long-term biocompatibility of Resilon. Conclusion: Our findings validate Resilon as an in vivo biocompatible material. However, our data suggest that long-term biocompatibility of Resilon, despite validated, is inferior to that of gutta-percha control.

  12. Particle morphology influence on mechanical and biocompatibility properties of injection molded Ti alloy powder.

    Science.gov (United States)

    Gülsoy, H Özkan; Gülsoy, Nagihan; Calışıcı, Rahmi

    2014-01-01

    Titanium and Titanium alloys exhibits properties that are excellent for various bio-applications. Metal injection molding is a processing route that offers reduction in costs, with the added advantage of near net-shape components. Different physical properties of Titanium alloy powders, shaped and processed via injection molding can achieve high complexity of part geometry with mechanical and bioactivity properties, similar or superior to wrought material. This study describes that the effect of particle morphology on the microstructural, mechanical and biocompatibility properties of injection molded Ti-6Al-4V (Ti64) alloy powder for biomaterials applications. Ti64 powders irregular and spherical in shape were injection molded with wax based binder. Binder debinding was performed in solvent and thermal method. After debinding the samples were sintered under high vacuum. Metallographic studies were determined to densification and the corresponding microstructural changes. Sintered samples were immersed in a simulated body fluid (SBF) with elemental concentrations that were comparable to those of human blood plasma for a total period of 15 days. Both materials were implanted in fibroblast culture for biocompatibility evaluations were carried out. The results show that spherical and irregular powder could be sintered to a maximum theoretical density. Maximum tensile strength was obtained for spherical shape powder sintered. The tensile strength of the irregular shape powder sintered at the same temperature was lower due to higher porosity. Finally, mechanical tests show that the irregular shape powder has lower mechanical properties than spherical shape powder. The sintered irregular Ti64 powder exhibited better biocompatibility than sintered spherical Ti64 powder. Results of study showed that sintered spherical and irregular Ti64 powders exhibited high mechanical properties and good biocompatibility properties. PMID:25201399

  13. Tribological study of lubricious DLC biocompatible coatings.

    Science.gov (United States)

    Brizuela, M; Garcia-Luis, A; Viviente, J L; Braceras, I; Oñate, J I

    2002-12-01

    DLC (diamond-like carbon) coatings have remarkable tribological properties due mainly to their good frictional behavior. These coatings can be applied in many industrial and biomedical applications, where sliding can generate wear and frictional forces on the components, such as orthopaedic metal implants. This work reports on the development and tribological characterization of functionally gradient titanium alloyed DLC coatings. A PVD-magnetron sputtering technique has been used as the deposition method. The aim of this work was to study the tribological performance of the DLC coating when metal to metal contact (cobalt chromium or titanium alloys) takes place under dry and lubricated test conditions. Prior work by the authors demonstrates that the DLC coating reduced considerably the wear of the ultra-high-molecular-weight polyethylene (UHMWPE). The DLC coating during mechanical testing exhibited a high elastic recovery (65%) compared to the values obtained from Co-Cr-Mo (15%) and Ti-6Al-4V (23%). The coating exhibited an excellent tribo-performance against the Ti-6Al-4V and Co-Cr-Mo alloys, especially under dry conditions presenting a friction value of 0.12 and almost negligible wear. This coating has passed biocompatibility tests for implant devices on tissue/bone contact according to international standards (ISO 10993). PMID:15348654

  14. Evaluation of iron oxide nanoparticle biocompatibility.

    Science.gov (United States)

    Hanini, Amel; Schmitt, Alain; Kacem, Kamel; Chau, François; Ammar, Souad; Gavard, Julie

    2011-01-01

    Nanotechnology is an exciting field of investigation for the development of new treatments for many human diseases. However, it is necessary to assess the biocompatibility of nanoparticles in vitro and in vivo before considering clinical applications. Our characterization of polyol-produced maghemite γ-Fe(2)O(3) nanoparticles showed high structural quality. The particles showed a homogeneous spherical size around 10 nm and could form aggregates depending on the dispersion conditions. Such nanoparticles were efficiently taken up in vitro by human endothelial cells, which represent the first biological barrier to nanoparticles in vivo. However, γ-Fe(2)O(3) can cause cell death within 24 hours of exposure, most likely through oxidative stress. Further in vivo exploration suggests that although γ-Fe(2)O(3) nanoparticles are rapidly cleared through the urine, they can lead to toxicity in the liver, kidneys and lungs, while the brain and heart remain unaffected. In conclusion, γ-Fe(2)O(3) could exhibit harmful properties and therefore surface coating, cellular targeting, and local exposure should be considered before developing clinical applications. PMID:21589646

  15. Biocompatibility index of Ag-containing antiseptics

    Directory of Open Access Journals (Sweden)

    Karkour, Yousef

    2006-08-01

    Full Text Available We defined a biocompatibility index (BI for comparing. The BI takes into account both the results of the in-vitro cytotoxicity, i.e. the concentration at which 50% of the cells are damaged, and the microbiocidal activity, i.e. the concentration at which the baseline burden of the test micro-organisms is reduced by at least 5-log steps. For AgNO3 and Ag-SD no BI value was calculable since the highest tested active substance concentration of 1% corresponding to the concentration for use results in no reduction in the baseline bacterial burden. However, the theoretical value should be much smaller than 0.002 All tested silver-containing preparations were not effective against Staphylococcus aureus using 30 min contact time. Using Escherichia coli as test microorganisms, colloidal silver in the form of mild silver protein (based on Ag has a BI value of 0.002, chlorhexidine 0.8, PVP-iodine (based on iodine 1.0, polihexanide 1.5, and octenidine 1.8, i.e. the Ag-containing preparations do not act antiseptically within 30 min of contact time. Therefore, depending from the kind of the wound in clinical practice Ag-containing preparations should be substituted by e.g. polihexanide-, octenidine- or PVP-iodine-containing preparations, which show in lower concentration than in the concentration in use microbicidal effect within 30 min.

  16. Biocompatible 3D Matrix with Antimicrobial Properties

    Directory of Open Access Journals (Sweden)

    Alberto Ion

    2016-01-01

    Full Text Available The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL, hydroxyapatite (HAp, β-cyclodextrin (β-CD and usnic acid (UA. The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM, Fourier Transform Infrared Microscopy (FT-IRM, Transmission Electron Microscopy (TEM, and X-ray Diffraction (XRD. In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D anti-infective regeneration matrix for bone tissue engineering.

  17. Controlled Growth of Biocompatible Polymers from Diamond Nanoparticles

    Czech Academy of Sciences Publication Activity Database

    Cígler, Petr; Řehoř, Ivan; Šlegerová, Jitka; Hrubý, Martin; Macková, Hana; Kučka, Jan; Filippov, Sergey K.

    San Francisco: -, 2013. ZZ10.04. [2013 MRS Spring Meeting & Exhibit. 01.04.2013-05.04.2013, San Francisco] Institutional support: RVO:61388963 ; RVO:61389013 Keywords : fluorescent nanodiamonds * biocompatible polymers Subject RIV: CC - Organic Chemistry

  18. Ti1-xAux Alloys: Hard Biocompatible Metals and Their Possible Applications

    Science.gov (United States)

    Svanidze, Eteri; Besara, Tiglet; Ozaydin, M. Fevzi; Xin, Yan; Han, Ke; Liang, Hong; Siegrist, Theo; Morosan, Emilia

    2015-03-01

    The search for new hard materials is often challenging from both theoretical and experimental points of view. Furthermore, using materials for biomedical applications calls for alloys with high biocompatibility which are even more sparse. The Ti1-xAux (0 . 22 dental, and prosthetic applications, where they could be used as both permanent and temporary components. Additionally, the ability of Ti1-xAux alloys to adhere to ceramic parts could reduce the weight and cost of these components. The work at Rice was supported by NSF DMR 0847681 (E.M. and E.S.).

  19. Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells

    OpenAIRE

    Leticia Boldrin MESTIERI; GOMES-CORNÉLIO, Ana Lívia; RODRIGUES, Elisandra Márcia; SALLES, Loise Pedrosa; BOSSO-MARTELO, Roberta; Juliane Maria GUERREIRO-TANOMARU; TANOMARU-FILHO, Mário

    2015-01-01

    Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3...

  20. Femtosecond pulsed laser deposition of biological and biocompatible thin layers

    Energy Technology Data Exchange (ETDEWEB)

    Hopp, B. [Hungarian Academy of Sciences, University of Szeged, Research Group on Laser Physics, Dom ter 9, H-6720 Szeged (Hungary)]. E-mail: bhopp@physx.u-szeged.hu; Smausz, T. [Hungarian Academy of Sciences, University of Szeged, Research Group on Laser Physics, Dom ter 9, H-6720 Szeged (Hungary); Kecskemeti, G. [Department of Optics and Quantum Electronics, University of Szeged, Dom ter 9, H-6720 Szeged (Hungary); Klini, A. [Institute of Electronic Structure and Laser (I.E.S.L.), Foundation for Research and Technology-Hellas (F.O.R.T.H.), P.O. Box 1527, GR-711 10 Heraklion, Crete (Greece); Bor, Zs. [Department of Optics and Quantum Electronics, University of Szeged, Dom ter 9, H-6720 Szeged (Hungary)

    2007-07-31

    In our study we investigate and report the femtosecond pulsed laser deposition of biological and biocompatible materials. Teflon, polyhydroxybutyrate, polyglycolic-acid, pepsin and tooth in the form of pressed pellets were used as target materials. Thin layers were deposited using pulses from a femtosecond KrF excimer laser system (FWHM = 450 fs, {lambda} = 248 nm, f = 10 Hz) at different fluences: 0.6, 0.9, 1.6, 2.2, 2.8 and 3.5 J/cm{sup 2}, respectively. Potassium bromide were used as substrates for diagnostic measurements of the films on a FTIR spectrometer. The pressure in the PLD chamber was 1 x 10{sup -3} Pa, and in the case of tooth and Teflon the substrates were heated at 250 deg. C. Under the optimized conditions the chemical structure of the deposited materials seemed to be largely preserved as evidenced by the corresponding IR spectra. The polyglycolic-acid films showed new spectral features indicating considerable morphological changes during PLD. Surface structure and thickness of the layers deposited on Si substrates were examined by an atomic force microscopy (AFM) and a surface profilometer. An empirical model has been elaborated for the description of the femtosecond PLD process. According to this the laser photons are absorbed in the surface layer of target resulting in chemical dissociation of molecules. The fast decomposition causes explosion-like gas expansion generating recoil forces which can tear off and accelerate solid particles. These grains containing target molecules without any chemical damages are ejected from the target and deposited onto the substrate forming a thin layer.

  1. Impact of trace elements on biocompatibility of titanium scaffolds

    International Nuclear Information System (INIS)

    A titanium oxide scaffold has recently been reported with high compressive strength (>2 MPa) which may allow its use in bone. However, would it be possible to enhance the scaffolds' performance by selecting a titanium oxide raw material without elemental contamination? Elements in implant surfaces have been reported to provoke implant failure. Thus, this study aims to compare different commercial titanium dioxide powders in order to choose the appropriate powder for scaffold making. The x-ray photoelectron spectroscopy (XPS) analysis identified the trace elements, mainly Al, Si, C, Ca and P. Cellular response was measured by cytotoxic effect, cell growth and cytokine secretion from murine preosteoblasts (MC3T3-E1) in vitro. The XPS data showed that traces of carbon-based molecules, silicon, nitrogen and aluminium in the powder were greatly reduced after cleaning in 1 M NaOH. As a result, reduction in cytotoxicity and inflammatory response was observed. Carbon contamination seemed to have a minor effect on the cellular response. Strong correlations were found between Al and Si contamination levels and the inflammatory response and cytotoxic effect. Thus, it is suggested that the concentration of these elements should be reduced in order to enhance the scaffolds' biocompatibility.

  2. Biocompatibility of new Ti-Nb-Ta base alloys.

    Science.gov (United States)

    Hussein, Abdelrahman H; Gepreel, Mohamed A-H; Gouda, Mohamed K; Hefnawy, Ahmad M; Kandil, Sherif H

    2016-04-01

    β-type titanium alloys are promising materials in the field of medical implants. The effect of β-phase stability on the mechanical properties, corrosion resistance and cytotoxicity of a newly designed β-type (Ti77Nb17Ta6) biocompatible alloys are studied. The β-phase stability was controlled by the addition of small quantities of Fe and O. X-ray diffraction and microstructural analysis showed that the addition of O and Fe stabilized the β-phase in the treated solution condition. The strength and hardness have increased with the increase in β-phase stability while ductility and Young's modulus have decreased. The potentio-dynamic polarization tests showed that the corrosion resistance of the new alloys is better than Ti-6Al-4V alloy by at least ten times. Neutral red uptake assay cytotoxicity test showed cell viability of at least 95%. The new alloys are promising candidates for biomedical applications due to their high mechanical properties, corrosion resistance, and reduced cytotoxicity. PMID:26838885

  3. Characterization and biocompatibility of fluoridated biphasic calcium phosphate ceramics

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Z.L. [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China); Yu, H.Y. [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China)], E-mail: yhyang6812@tfol.com; Zeng, Q. [Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041 (China); He, H.W. [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China)

    2008-11-15

    Biphasic calcium phosphate ceramics (BCP) has been widely used in tooth and bone implants due to its excellent biocompatibility. Incorporation of fluorine ions in BCP has drawn much attention because of the beneficial role played by the fluorine ions in bone and tooth growth. The aim of this study was to obtain fluoridated biphasic calcium phosphate (FBCP) by immersing BCP into saturated ZnF{sub 2} solution with F{sup -} concentration of 3500 mg/l at different times. The phase and incorporation of fluoride into BCP were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The biomineralization and influence of FBCP on osteoblastic behavior were evaluated and compared with that of biphasic calcium phosphate (BCP). The results exhibited that the phase evolution of the BCP was affected by the fluoride incorporation and the FBCP significantly improved the differentiation and proliferation of osteoblasts. These findings suggest that the FBCP would be very useful as a bone reconstructive material.

  4. Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition.

    Science.gov (United States)

    Park, Dong-Soo; Kim, In-Sook; Kim, Hyeongil; Chou, Alex Hung Kuo; Hahn, Byung-Dong; Li, Long-Hao; Hwang, Soon-Jung

    2010-08-01

    Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant. PMID:20574972

  5. Reinforcement of bacterial cellulose aerogels with biocompatible polymers.

    Science.gov (United States)

    Pircher, N; Veigel, S; Aigner, N; Nedelec, J M; Rosenau, T; Liebner, F

    2014-10-13

    Bacterial cellulose (BC) aerogels, which are fragile, ultra-lightweight, open-porous and transversally isotropic materials, have been reinforced with the biocompatible polymers polylactic acid (PLA), polycaprolactone (PCL), cellulose acetate (CA), and poly(methyl methacrylate) (PMMA), respectively, at varying BC/polymer ratios. Supercritical carbon dioxide anti-solvent precipitation and simultaneous extraction of the anti-solvent using scCO2 have been used as core techniques for incorporating the secondary polymer into the BC matrix and to convert the formed composite organogels into aerogels. Uniaxial compression tests revealed a considerable enhancement of the mechanical properties as compared to BC aerogels. Nitrogen sorption experiments at 77K and scanning electron micrographs confirmed the preservation (or even enhancement) of the surface-area-to-volume ratio for most of the samples. The formation of an open-porous, interpenetrating network of the second polymer has been demonstrated by treatment of BC/PMMA hybrid aerogels with EMIM acetate, which exclusively extracted cellulose, leaving behind self-supporting organogels. PMID:25037381

  6. Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid films

    Energy Technology Data Exchange (ETDEWEB)

    Coll Ferrer, M. Carme [Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104 (United States); Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104 (United States); Eckmann, Uriel N. [Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104 (United States); Composto, Russell J. [Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104 (United States); Eckmann, David M., E-mail: eckmanndm@uphs.upenn.edu [Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, PA 19104 (United States)

    2013-11-01

    In previous work, we developed novel antibacterial hybrid coatings based on dextran containing dispersed Ag NPs (∼ 5 nm, DEX-Ag) aimed to offer dual protection against two of the most common complications associated with implant surgery, infections and rejection of the implant. However, their blood-material interactions are unknown. In this study, we assess the hemocompatibility and biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-inflammatory eicosanoid expression (Prostaglandin E{sub 2}, PGE{sub 2}) and release of reactive oxygen species (ROS, superoxide and H{sub 2}O{sub 2}) following incubation of the cells with the surfaces. The morphology and cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS, the expression of pro-inflammatory cytokines remained minimal with similar levels of PGE{sub 2}, as compared to the other surfaces studied. These results highlight low toxicity of DEX-Ag and hold promise for future applications in vivo. - Highlights: • We examined specific blood-contact reactions of dextran doped with Ag NPs coatings. • Biocompatibility was assessed with THP-1 cells and PMA-stimulated THP-1 cells. • Glass, polyurethane and dextran were used as reference surfaces. • Hybrid coatings exhibited non-hemolytic properties. • Low toxicity, inflammatory response and ROS suggest potential for in vivo use.

  7. Biocompatible peritoneal dialysis solutions: many questions but few answers.

    Science.gov (United States)

    Blake, Peter G; Jain, Arsh K; Yohanna, Sechelle

    2013-11-01

    The clinical value of biocompatible peritoneal dialysis solutions is uncertain because of inconsistent findings in randomized controlled trials. A systematic review by Cho et al. examining 20 such trials suggests a beneficial effect on residual renal function. However, the finding is not robust and may relate to decreased ultrafiltration causing hypervolemia. Future prescribing of these biocompatible solutions will probably continue to be driven by opinion, marketing, and cost considerations rather than by evidence-based medicine. PMID:24172730

  8. Microstructural Properties of Biocompatible Nanofibrous Membranes Prepared by Electrospinning Technique

    Czech Academy of Sciences Publication Activity Database

    Soukup, Karel; Hejtmánek, Vladimír; Petráš, D.; Šolcová, Olga

    - : -, 2013, s. 525. ISBN N. [European Congress of Chemical Engineering /9./. The Hague (NL), 21.04.2013-25.04.2013] R&D Projects: GA ČR GPP106/11/P459; GA ČR(CZ) GAP204/11/1206 Institutional support: RVO:67985858 Keywords : electrospinning * biocompatible membranes * biocompatible membranes * adsorption-desorption measurements Subject RIV: CI - Industrial Chemistry, Chemical Engineering http://www.ecce2013.eu/index.php

  9. Synthesis of biocompatible polymers by plasma; Sintesis de polimeros biocompatibles por plasma

    Energy Technology Data Exchange (ETDEWEB)

    Colin O, E

    2007-07-01

    In this work biocompatible polymers were synthesized by plasma based on pyrrole, ethyleneglycol and allylamine. These monomers are biologically important because they contain oxygen and nitrogen in their structure and they form bonding like; N-H, C-N, C-O and O-H that are also in the human system. The polymers were synthesized with splendor electric discharges to 13.5 MHz, among 10 and 100 W, resistive coupling, pressure of 10{sup -1} mbar and 180 minutes of reaction. The interaction of the biological systems with biomaterials depends in many cases of the properties that present the surfaces, because the rough and/or porous surfaces favor the adherence of cells. The results indicate that the ruggedness of the polymers can be controlled with the synthesis energy, since when modifying it flat and/or rough surfaces they are obtained. The compatibility of water with other solutions that it is a form of increasing the adhesion of cells with biopolymers. The affinity with water and solutions is evaluated calculating the contact angle of the polymers surface with drops of concentration solutions and similar composition to the extracellular liquid of the spinal marrow of the human body. The solutions that were proven were based on NaCl, NaCl-MgSO{sub 4}, and a mixture Krebs-Ringer that has chemical composition and similar concentration to that of the fluids of the spinal marrow. In the Poly pyrrole (PPy)/Polyethyleneglycol (PEG) copolymer, the biggest angles corresponded to the Krebs-Ringer solution, in the interval of 18 to 14 degrees and those lowest to the NaCl solution, of 14.5 at 11 degrees. The Poly allylamine had the more high values with water in the interval of 16.5 to 12.5 degrees and those lowest with the NaCl solution, of 13 at 9.5 degrees. On the other hand, in the derived polymers of pyrrole the more high values corresponded to the treatment with water, until 37, and those lowest to the NaCl-MgSO{sub 4} solution, up to 10. The solutions where participated Na

  10. Impregnation of silver sulfadiazine into bacterial cellulose for antimicrobial and biocompatible wound dressing

    International Nuclear Information System (INIS)

    Silver sulfadiazine (SSD) is a useful antimicrobial agent for wound treatment. However, recent findings indicate that conventional SSD cream has several drawbacks for use in treatments. Bacterial cellulose (BC) is a promising material for wound dressing due to its outstanding properties of holding water, strength and degradability. Unfortunately, BC itself exhibits no antimicrobial activity. A combination of SSD and BC is envisaged to form a new class of wound dressing with both antimicrobial activity and biocompatibility, which has not been reported to date. To achieve antimicrobial activity, SSD particles were impregnated into BC by immersing BC into SSD suspension after ultrasonication, namely SSD–BC. Parameters influencing SSD–BC impregnation were systematically studied. Optimized conditions of sonication time for no less than 90 min and the proper pH value between 6.6 and 9.0 were suggested. The absorption of SSD onto the BC nanofibrous network was revealed by XRD and SEM analyses. The SSD–BC membranes exhibited significant antimicrobial activities against Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus evaluated by the disc diffusion method. In addition, the favorable biocompatibility of SSD–BC was verified by MTT colorimetry, epidermal cell counting method and optical microscopy. The results demonstrate the potential of SSD–BC membranes as a new class of antimicrobial and biocompatible wound dressing. (paper)

  11. Alginate/Poly(γ-glutamic Acid Base Biocompatible Gel for Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Wing P. Chan

    2015-01-01

    Full Text Available A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid, alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca2+ from Ca–γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.

  12. The mechanics and biocompatibility characteristics of carbon nanotubes-polyurethane composite membranes:a preliminary study

    International Nuclear Information System (INIS)

    Objective: To discuss the mechanics and biocompatibility characteristics of carbon nanotubes-polyurethane composite membranes. Methods: The mechanics property of carbon nanotubes-polyurethane composite membranes with different carbon nanotubes contents were tested by universal material testing machine. The surface of the membranes was observed by electron microscope when the stent was bent 90 degree. And its cytotoxicity was tested by cultivating study with 7721 cell. The metallic stent that was covered with carbon nanotubes-polyurethane composite membrane by using dip-coating method was inserted in rabbit esophagus in order to evaluate its biocompatibility in vivo. Results: Composite membranes tensile strength (MPa) and elongation at break (%) were 4.62/900, 6.05/730, 8.26/704 and 5.7/450 when the carbon nanotubes contents were 0%, 0.1%, 0.3% and 0.5%, respectively. If the stent was bent at 90 degree, its surface was still smooth without any fractures when it was scanned by electron microscope.Composite membranes had critical cytotoxicity when its carbon nanotubes content was up to 0.5% and 1.0%. No fissure nor degradation of composite membranes occurred at 30 days after composite membrane covered metallic stent was inserted in rabbit esophagus. Conclusion: When moderate carbon nanotubes are added into polyurethane composite membrane, the mechanics and biocompatibility characteristics of the polyurethane composite membrane can be much improved. (authors)

  13. Capsular contracture by silicone breast implants: possible causes, biocompatibility, and prophylactic strategies

    Directory of Open Access Journals (Sweden)

    Steiert AE

    2013-12-01

    Full Text Available Andreas E Steiert, Maria Boyce, Heiko Sorg Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany Abstract: The most common implanted material in the human body consists of silicone. Breast augmentation and breast reconstruction using silicone-based implants are procedures frequently performed by reconstructive and aesthetic surgeons. A main complication of this procedure continues to be the development of capsular contracture (CC, displaying the result of a fibrotic foreign body reaction after the implantation of silicone. For many years, experimental and clinical trials have attempted to analyze the problem of its etiology, treatment, and prophylaxis. Different theories of CC formation are known; however, the reason why different individuals develop CC in days or a month, or only after years, is unknown. Therefore, we hypothesize that CC formation, might primarily be induced by immunological mechanisms along with other reasons. This article attempts to review CC formation, with special attention paid to immunological and inflammatory reasons, as well as actual prophylactic strategies. In this context, the word “biocompatibility” has been frequently used to describe the overall biological innocuousness of silicone in the respective studies, although without clear-cut definitions of this important feature. We have therefore developed a new five-point scale with distinct key points of biocompatibility. Hence, this article might provide the basis for ongoing discussion in this field to reduce single-publication definitions as well as increase the understanding of biocompatibility. Keywords: biofilm, foreign body reaction, breast augmentation, biocompatibility, fibrosis

  14. Biocompatibility assessment of rice husk-derived biogenic silica nanoparticles for biomedical applications.

    Science.gov (United States)

    Alshatwi, Ali A; Athinarayanan, Jegan; Periasamy, Vaiyapuri Subbarayan

    2015-02-01

    Synthetic forms of silica have low biocompatibility, whereas biogenic forms have myriad beneficial effects in current toxicological applications. Among the various sources of biogenic silica, rice husk is considered a valuable agricultural biomass material and a cost-effective resource that can provide biogenic silica for biomedical applications. In the present study, highly pure biogenic silica nanoparticles (bSNPs) were successfully harvested from rice husks using acid digestion under pressurized conditions at 120°C followed by a calcination process. The obtained bSNPs were subjected to phase identification analysis using X-ray diffraction, which revealed the amorphous nature of the bSNPs. The morphologies of the bSNPs were observed using transmission electron microscopy (TEM), which revealed spherical particles 10 to 30 nm in diameter. Furthermore, the biocompatibility of the bSNPs with human lung fibroblast cells (hLFCs) was investigated using a viability assay and assessing cellular morphological changes, intracellular ROS generation, mitochondrial transmembrane potential and oxidative stress-related gene expression. Our results revealed that the bSNPs did not have any significant incompatibility in these in vitro cell-based approaches. These preliminary findings suggest that bSNPs are biocompatible, could be the best alternative to synthetic forms of silica and are applicable to food additive and biomedical applications. PMID:25492167

  15. Effects of graphene plates’ adoption on the microstructure, mechanical properties, and in vivo biocompatibility of calcium silicate coating

    OpenAIRE

    Xie YT; Li HQ; Ding CX; Zheng XB; Li K

    2015-01-01

    Youtao Xie, Hongqin Li, Chuanxian Ding, Xuebin Zheng, Kai Li Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai, People’s Republic of China Abstract: Calcium silicate (CS) ceramic is a good coating candidate for biomedical implants to improve biocompatibility and accelerate early osseo-integration. However, the poor fracture toughness and wear resistance of this ceramic material restricts the long-term performance of impl...

  16. Coating with Autologous Plasma Improves Biocompatibility of Mesh Grafts In Vitro: Development Stage of a Surgical Innovation

    OpenAIRE

    Holger Gerullis; Evangelos Georgas; Christoph Eimer; Christian Arndt; Dimitri Barski; Bernhard Lammers; Bernd Klosterhalfen; Mihaly Borós; Thomas Otto

    2013-01-01

    Purpose. To investigate mesh coating modalities with autologous blood components in a recently developed in vitro test system for biocompatibility assessment of alloplastic materials. Materials and Methods. Seven different mesh types, currently used in various indications, were randomly investigated. Meshes were coated prior to cultivation with autologous peripheral blood mononuclear cells (PBMCs), platelets, and blood plasma. Pretreated meshes were incubated over 6 weeks in a minced tissue a...

  17. Biocompatibility assessment of rice husk-derived biogenic silica nanoparticles for biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Alshatwi, Ali A., E-mail: alshatwi@ksu.edu.sa; Athinarayanan, Jegan; Periasamy, Vaiyapuri Subbarayan

    2015-02-01

    Synthetic forms of silica have low biocompatibility, whereas biogenic forms have myriad beneficial effects in current toxicological applications. Among the various sources of biogenic silica, rice husk is considered a valuable agricultural biomass material and a cost-effective resource that can provide biogenic silica for biomedical applications. In the present study, highly pure biogenic silica nanoparticles (bSNPs) were successfully harvested from rice husks using acid digestion under pressurized conditions at 120 °C followed by a calcination process. The obtained bSNPs were subjected to phase identification analysis using X-ray diffraction, which revealed the amorphous nature of the bSNPs. The morphologies of the bSNPs were observed using transmission electron microscopy (TEM), which revealed spherical particles 10 to 30 nm in diameter. Furthermore, the biocompatibility of the bSNPs with human lung fibroblast cells (hLFCs) was investigated using a viability assay and assessing cellular morphological changes, intracellular ROS generation, mitochondrial transmembrane potential and oxidative stress-related gene expression. Our results revealed that the bSNPs did not have any significant incompatibility in these in vitro cell-based approaches. These preliminary findings suggest that bSNPs are biocompatible, could be the best alternative to synthetic forms of silica and are applicable to food additive and biomedical applications. - Highlights: • Simple, rapid and convenient process • Amorphous and spherical with 10–30 nm size SiO{sub 2} nanoparticles were fabricated. • Biogenic silica nanoparticles showed biocompatibility. • bSNPs are an alternative to synthetic forms of silica.

  18. In vitro evaluation of biocompatibility of Ti-Mo-Sn-Zr superelastic alloy.

    Science.gov (United States)

    Nunome, Shoko; Kanetaka, Hiroyasu; Kudo, Tada-aki; Endoh, Kazuki; Hosoda, Hideki; Igarashi, Kaoru

    2015-07-01

    Shape memory alloys (SMAs) including superelastic alloys have unique properties such as shape memory and superelasticity, thus they are recognized as very useful biomaterials. These properties are very advantageous for medical use, and actually the SMA wires have been widely used in medical field. However, biocompatibility of nickel-titanium (Ni-Ti) alloy, which is the only practical SMA at present, has been questioned because of its high nickel content. The aim of this study was to evaluate the biocompatibility of a newly developed Ni-free Ti-based SMA for medical use. The newly developed SMA made of Ti-Mo-Sn-Zr system was processed into a disk of 15.1 mm in diameter. Pure titanium of the same shape was prepared as control. All the disk surfaces were polished using emery papers, #120, #400, and #600. Scanning electron microscopy and a 3D optics profiler were used to evaluate the surface of the materials. In vitro evaluations included colony examination for evaluation of the cell cytotoxicity, DNA quantification for the cell proliferation, Alamar blue assay for metabolic activity, FDA staining for the live cell imaging, and cell cycle analysis, using Chinese hamster fibroblastic V-79 cells and mouse osteoblastic MC3T3-E1 cells. In colony examination and DNA quantification, there was no significant difference between the Ti-Mo-Sn-Zr and the pure titanium. In FDA staining, cultured cells on the Ti-Mo-Sn-Zr alloy showed the same biocompatibility as those on the pure titanium. The present results suggest that the newly developed Ti-Mo-Sn-Zr alloy showed the high biocompatibility comparable to pure titanium and can be used as efficient biomaterial for medical use. PMID:25659946

  19. In Vitro Study of Biocompatibility and Toxicity of Magnesium Nanomaterials for Biodegradable Implants

    Science.gov (United States)

    Pallavi, Manishi

    Biodegradable magnesium (Mg) has a great potential to be used as a next generation implant material for orthopedic applications due to its mechanical and osseointegration properties. However, surface characteristics, biocompatibility and toxicity of the released corrosion products, in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg (OH)2) nanoparticles (NPs), at the junction of implants, and their surrounding tissues is not completely understood. Therefore, our goal was to identify in vitro biocompatibility, and toxicity of magnesium nanomaterials in osteoblast cells to mimic the in vivo environment for biodegradable implants. We hypothesized that the release of hydroxide ion (OH-) from MgO/ Mg(OH) 2 NPs will increase the corrosion behavior of these particles in osteoblast cells, and will introduce cytotoxicity. Therefore, the objective of this study was to characterize MgO/ Mg(OH)2 NPs in osteoblast cells, and to develop an electric cell-substrate impedance sensing (ECIS) system to measure the biocompatibility and toxicity of these particles in osteoblast cells. The corrosion behavior of the samples was analyzed through immersion test. The morphological characterization and element distribution of the surface corrosion products of the samples was performed using scanning electron microscopy (SEM) and electron dispersive X-ray spectroscopy (EDX), respectively. Cell viability and cytotoxicity of the samples was studied by live-dead assay. With ECIS system, biocompatibility and cytotoxicity of the samples was analyzed. Results shows that less than or equal to 1 mM concentrations of MgO/ Mg(OH) 2 NPs has negligible toxic effects on osteoblast cells. Therefore, this study provides a foundational knowledge for an acceptable range of these corrosion products that might release from the magnesium-based implants in the physiological environment, in order to understand the implant degradation for future in vivo study.

  20. Biocompatibility assessment of rice husk-derived biogenic silica nanoparticles for biomedical applications

    International Nuclear Information System (INIS)

    Synthetic forms of silica have low biocompatibility, whereas biogenic forms have myriad beneficial effects in current toxicological applications. Among the various sources of biogenic silica, rice husk is considered a valuable agricultural biomass material and a cost-effective resource that can provide biogenic silica for biomedical applications. In the present study, highly pure biogenic silica nanoparticles (bSNPs) were successfully harvested from rice husks using acid digestion under pressurized conditions at 120 °C followed by a calcination process. The obtained bSNPs were subjected to phase identification analysis using X-ray diffraction, which revealed the amorphous nature of the bSNPs. The morphologies of the bSNPs were observed using transmission electron microscopy (TEM), which revealed spherical particles 10 to 30 nm in diameter. Furthermore, the biocompatibility of the bSNPs with human lung fibroblast cells (hLFCs) was investigated using a viability assay and assessing cellular morphological changes, intracellular ROS generation, mitochondrial transmembrane potential and oxidative stress-related gene expression. Our results revealed that the bSNPs did not have any significant incompatibility in these in vitro cell-based approaches. These preliminary findings suggest that bSNPs are biocompatible, could be the best alternative to synthetic forms of silica and are applicable to food additive and biomedical applications. - Highlights: • Simple, rapid and convenient process • Amorphous and spherical with 10–30 nm size SiO2 nanoparticles were fabricated. • Biogenic silica nanoparticles showed biocompatibility. • bSNPs are an alternative to synthetic forms of silica

  1. Polymer surface adsorption as a strategy to improve the biocompatibility of graphene nanoplatelets.

    Science.gov (United States)

    Pinto, Artur M; Moreira, J Agostinho; Magalhães, Fernão D; Gonçalves, Inês C

    2016-10-01

    The biointeractions of graphene-based materials depend on their physico-chemical properties. These properties can be manipulated by polymer adsorption. Graphene nanoplatelets (GNP-C) were modified with PVA, HEC, PEG, PVP, chondroitin, glucosamine, and hyaluronic acid. These materials were characterized by SEM, DLS, XPS, Raman spectroscopy, and TGA. Surface adsorption was confirmed for all polymers. Biocompatibility evaluation showed that all of these materials induced low haemolysis (<1.7%) at concentrations up to 500μgmL(-1). GNP-C-PVA and GNP-C-HEC presented the lowest haemolysis percentages and were therefore more thoroughly studied. The morphology of HFF-1 cells was investigated by microscopy (optical, fluorescence, TEM) in order to evaluate interactions with GNP materials. Small GNP-C nanoplatelets were observed to enter cells independently of the surface treatment. For pristine GNP-C at a concentration of 50μgmL(-1), ROS production increased 4.4-fold. This effect is lower for GNP-C-PVA (3.3-fold) and higher for GNP-C-HEC (5.1-fold). Resazurin assays showed that GNP-C caused toxicity in HFF-1 cells at concentrations above 20μgmL(-1) at 24h, which decreased at 48 and 72h. PVA surface adsorption rendered GNP-C non-toxic at concentrations up to 50μgmL(-1). LIVE/DEAD assays showed that at 20 and 50μgmL(-1) cell death is significantly lower for GNP-C-PVA compared to pristine GNP-C. Modification of nanoplatelets with HEC resulted in no benefit in terms of biocompatibility, whereas PVA considerably improved the biocompatibility. PMID:27451370

  2. Evaluation of biocompatibility of Targis Dentin and Artglass by using subcutaneous implantation test

    Directory of Open Access Journals (Sweden)

    Sonmez Nalan

    2010-01-01

    Full Text Available Introduction: Biocompatibility of a crown-bridge material is as important as its physical and mechanical properties. It is also one of the most important factors for the long-lasting clinical success of that restoration. It directly contacts the vital prepared tooth and that is the reason it has to be nontoxic to the local tissues, such as the pulp, gingiva, or the rest of the body. Materials with different physical properties are used in the conventional fixed prosthodontic restorations. Recently, metal-free systems that are reinforced with fibers have been improved for crown and bridge restorations. These new composite systems have the advantages of both ceramic and polymer chemistry. Materials and Methods: In this research, biocompatibility of two ceramic-polymer-based prosthetic materials (Targis Dentin® and Artglass Dentin® was studied using a subcutaneous implantation test on rats. Initially (15 th day mild inflammatory reactions were observed in tissues, which directly contacted the Artglass, Targis, and control tubes. These probably originated from the surgical traumas. After the 90th day of implantation, these reactions resolved and healthy, well-organized fibrous connective capsules were seen around the implants. Results: Initially (15 th day mild inflammatory reactions were observed in tissues, which directly contacted the Artglass, Targis, and control tubes. These probably originated from the surgical traumas. After the 90 th day of implantation, these reactions resolved and healthy, well-organized fibrous connective capsules were seen around the implants. Conclusion: At the end of the study, according to the FDI and ISO-7405 standards, Targis and Artglass indicated biocompatibility with the subcutaneous connective tissue of the rat.

  3. Surface Modification of Biodegradable Polymers towards Better Biocompatibility and Lower Thrombogenicity.

    Directory of Open Access Journals (Sweden)

    Andreas Rudolph

    Full Text Available Drug-eluting stents (DES based on permanent polymeric coating matrices have been introduced to overcome the in stent restenosis associated with bare metal stents (BMS. A further step was the development of DES with biodegradable polymeric coatings to address the risk of thrombosis associated with first-generation DES. In this study we evaluate the biocompatibility of biodegradable polymer materials for their potential use as coating matrices for DES or as materials for fully bioabsorbable vascular stents.Five different polymers, poly(L-lactide PLLA, poly(D,L-lactide PDLLA, poly(L-lactide-co-glycolide P(LLA-co-GA, poly(D,L-lactide-co-glycolide P(DLLA-co-GA and poly(L-lactide-co-ε-caprolactone, P(LLA-co-CL were examined in vitro without and with surface modification. The surface modification of polymers was performed by means of wet-chemical (NaOH and ethylenediamine (EDA and plasma-chemical (O2 and NH3 processes. The biocompatibility studies were performed on three different cell types: immortalized mouse fibroblasts (cell line L929, human coronary artery endothelial cells (HCAEC and human umbilical vein endothelial cells (HUVEC. The biocompatibility was examined quantitatively using in vitro cytotoxicity assay. Cells were investigated immunocytochemically for expression of specific markers, and morphology was visualized using confocal laser scanning (CLSM and scanning electron (SEM microscopy. Additionally, polymer surfaces were examined for their thrombogenicity using an established hemocompatibility test.Both endothelial cell types exhibited poor viability and adhesion on all five unmodified polymer surfaces. The biocompatibility of the polymers could be influenced positively by surface modifications. In particular, a reproducible effect was observed for NH3-plasma treatment, which enhanced the cell viability, adhesion and morphology on all five polymeric surfaces.Surface modification of polymers can provide a useful approach to enhance their

  4. Biocompatibility evaluation in vitro. Part Ⅲ: Cytotoxicity expression of human and animal osteoblasts on the biomaterials

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Exclusion and imflammation in the clinic are observed for various reasons including material chemical composition, physical properties as well as macro- and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue in current biocompatibility to screen the potential implant materials and drugs. This study was aimed at investigation reaction between the potential implant materials and surround tissue. Cytotoxicity of human and mt osteoblast in the material extracts was determinated by testing standards such as GHS assay, MTI assay, alkaline phosphatase activity assay, LDH assay, and Lowry assay. Research results demonstrated that compared with the control condition polystyrene culture plate both human and rat osteoblast cells have normal phenotypie expression in hydroxyapatite extract, and this expression was statistically restricted in hydroxyapatite-spinel extract. However, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.

  5. The in vitro and in vivo evaluation of the biocompatibility of Mg alloys

    International Nuclear Information System (INIS)

    Magnesium (Mg) and its alloys are being widely investigated for their potential use as resorbable biomaterials for orthopaedic applications. However, the natural corrosion of the metals results in potentially harmful perturbations to the physiological environment, which requires a comprehensive understanding of their biocompatibility. Currently, most investigations proceed directly from in vitro biocompatibility studies to intraosseous implantation. However, this can result in the unnecessary elimination of appropriate materials due to over sensitive in vitro methods or the implantation of potentially harmful materials. This study involved the development of a relevant in vitro cell culture method, and an in vivo soft tissue implantation technique to provide an intermediate step between basic cell culture methods and large animal intraosseous investigations. A Live/Dead fluorescent assay was used to investigate the viability of both L929 and SaOS-2 cells exposed to Mg alloys, with the results compared to those seen with the intramuscular implantation of the same materials in Lewis rats. These methods were able to successfully provide data on the corrosion of Mg alloys, allowing the identification of slowly and safely corroding materials that may be used in future intraosseous investigations. (paper)

  6. Principles of Manufacturing Biocompatible and Biostable Polymer Implants (Review

    Directory of Open Access Journals (Sweden)

    V.М. Treushnikov

    2015-09-01

    Full Text Available The review concerns the basic theoretical aspects of manufacturing biocompatible and biostable implants and represents, mainly, the experience of our research team. Biocompatible implants have been shown to be understood to mean both: those, which are not rejected by the body as well as those, which are not capsulated in the body. They are to be fabricated according to one-stage frontal photopolymerization with shallow reaction front to avoid defect formation in a polymer. Moreover, an additional operation/stage is required to result in the death of end free macroradicals and labile products in a polymer. For implant fabrication we used photopolimerizable compositions resulting in the formation of hydrophobic spatially cross-linked polymers, their correlation time of rotational motion of a paramagnetic probe of 2,2,6,6-tetramethyl-4-oxypiperidine-1-oxide approximately being 6·10–10 s. The fulfillment of these conditions means the use of radically polymerizable oligomer-based compositions (oligoester methacrylates, oligocarbonate methacrylates, oligourethane methacrylates, etc.. Compositions having lower or higher correlation time of the specified probe are not appropriate for the fabrication of biocompatible and biostable implants. The characteristics of oligomer-monomer compounds have a greater effect on physicochemical properties of implants rather than on their biocompatibility and biostability. No implant incapsulation is determined by the initial composition formulation provided that the mentioned conditions are fulfilled. A polymer in biostable and biocompatible implants can be only optically transparent, though the converse is not necessary.

  7. In vitro biocompatibility evaluation of silk-fibroin/polyurethane membrane with cultivation of HUVECs

    Science.gov (United States)

    Zhou, Mei; Wang, Wei-Ci; Liao, Yong-Gui; Liu, Wen-Qi; Yu, Miao; Ouyang, Chen-Xi

    2014-03-01

    In order to investigate the in vitro biocompatibility of a novel polyurethane (PU) membrane modified by incorporation of superfine silk-fibroin powder (SFP), which was prepared for small-diameter vascular grafts, with the cultivation of human umbilical vein endothelial cells (HUVECs), PU and SFP were mixed with the ratios of 9:1, 7:3, 5:5, 3:7 (PU:SFP) to make four composite materials. Unmodified PU and polytetrafluoroethylene (PTFE) were added as control groups. CCK-8 assay was used to evaluate the cytotoxicity of these biomaterials. Data were processed using SPSS, and P < 0.05 was considered to be statistically significant. Adherence and spreading of HUVECs on the surface of specimens was observed using direct contact cultivation. The toxicity ratings of the novel composites were grade 0-1, which is in the acceptable range. In all the experimental groups except control, SFP/PU with ratio of 1:9 had the least cytotoxicity property, and more content of SFP in the composite showed no improvement of the biocompatibility. HUVECs strongly attached to and grew on the surface of the biomaterials, and proliferated rapidly. The proliferation ability increased with increased proportion of SFP; however the cell quantity on the surface of the materials decreased when the proportion of SFP was equal to or larger than that of PU in the composite. It is concluded that this novel material has excellent cellular affinity with no cytotoxicity to HUVECs. Adding SFP gives PU better biocompatibility, while further research on optimum blend ratios is still needed.

  8. Synthesis, characterization and biocompatibility evaluation of hydroxyapatite - gelatin polyLactic acid ternary nanocomposite

    OpenAIRE

    Z. Nabipour; M.S. Nourbakhsh; M. Baniasadi

    2016-01-01

    Objective(s): The current study reports the production and biocompatibility evaluation of a ternary nanocomposite consisting of HA, PLA, and gelatin for biomedical application.Materials and Methods: Hydroxyapatite nanopowder (HA: Ca10(PO4)6(OH)2) was produced by burning the bovine cortical bone within the temperature range of 350-450 oC followed by heating in an oven at 800. Synthesis of the ternary nanocomposite was carried out in two steps: synthesis of gelatin-hydroxyapatite binary nanocom...

  9. Structural and degradation studies of a biocompatible Zn-L-tartrate metal-organic framework

    OpenAIRE

    2015-01-01

    New anhydrous Zn-l-tratrate was synthesized under hydrothermal conditions. Crystal structure which was solved by powder X-ray diffraction data from monoclinic unit cell (I2, a=11.7557(1), b=9.0319(1), c=5.0750(1), β=91.920(1)) consists of the three-dimensional framework where each Zn atom coordinates four tartrate ligands in distorted octahedral geometry. Due to its biocompatible components, the degradation of material was studied in aqueous media under different pH values (pH=1–7) and in the...

  10. Biocompatible Polyhydroxyethylaspartamide-based Micelles with Gadolinium for MRI Contrast Agents

    OpenAIRE

    Kim Hyo Jeong; Lee Ha-Young; Seong Hasoo; Shin Byung Cheol; Yuk Soon Hong; Cho Sun Hang; Kwak Byung-Kook; Jeong Sang Young; Hwang Sung-Joo

    2010-01-01

    Abstract Biocompatible poly-[N-(2-hydroxyethyl)-d,l-aspartamide]-methoxypoly(ethyleneglycol)-hexadecylamine (PHEA-mPEG-C16) conjugated with 1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid-gadolinium (DOTA-Gd) via ethylenediamine (ED) was synthesized as a magnetic resonance imaging (MRI) contrast agent. Amphiphilic PHEA-mPEG-C16-ED-DOTA-Gd forms micelle in aqueous solution. All the synthesized materials were characterized by proton nuclear magnetic resonance (1H NMR). Micelle size and ...

  11. Enhancement of biocompatibility of nickel-titanium by laser surface modification technology

    Science.gov (United States)

    Ng, Ka Wai

    Nickel Titanium is a relatively new biomaterial that has attracted research interest for biomedical application. The good biocompatibility with specific functional properties of shape memory effect and superelasticity creates a smart material for medical applications. However, there are still concerns on nickel ion release of this alloy if it is going to be implanted for a long time. Nickel ion is carcinogenic and also causes allergic response and degeneration of muscle tissue. The subsequent release of Ni+ ions into the body system is fatal for the long term application of this alloy in the human body. To improve the long term biocompatibility and corrosion properties of NiTi, different surface treatment techniques have been investigated but no optimum technique has been established yet. This project will investigate the feasibility of applying laser surface alloying technique to improve the corrosion resistance and biocompatibility of NiTi in simulated body fluid condition. This thesis summarizes the result of laser surface modification of NiTi with Mo, Nb and Co using CO2 laser. The modified layer, which is free of microcracks and pores, acts as physical barrier to reduce nickel release and enhance the surface properties. The hardness values of the Mo-alloyed NiTi, Nb-alloyed NiTi and Co-alloyed NiTi surface were found to be three to four times harder than the NiTi substrate. Corrosion polarization tests also showed that the alloyed NiTi are significantly more resistant than the NiTi alloy. The release of Ni ions can be greatly reduced after laser surface alloying NiTi with Mo, Nb and Co. The improvement in wettability characteristics, the growth of the apatite on the specimen's surface and the adhesion of cell confirm the good biocompatibility after laser surface alloying. It is concluded that laser surface alloying is one of the potential technique not only to improve the corrosion resistance with low nickel release rate, but also retain the good

  12. Solubility of dense CO2 in two biocompatible acrylate copolymers

    Directory of Open Access Journals (Sweden)

    A. R. C. Duarte

    2006-06-01

    Full Text Available Biocompatible polymers and copolymers are frequently being used as part of controlled delivery systems. These systems can be prepared using a "clean and environment friendly" technology like supercritical fluids. One great advantage of this process is that compressed carbon dioxide has excellent plasticizing properties and can swell most biocompatible polymeric matrixes, thus promoting drug impregnation processes. Mass sorption of two acrylate biocompatible copolymers contact with supercritical carbon dioxide is reported. Equilibrium solubility of dense carbon dioxide in poly(methylmethacrylate-co-ethylhexylacrylate and poly(methylmethacrylate-co-ethylhexylacrylate-co-ethyleneglycoldimethacrylate was studied by a static method at 10.0 MPa and 313 K. The reticulated copolymer had Fickean behavior and its diffusion coefficient was calculated, under operating conditions.

  13. Cysteine modified polyaniline films improve biocompatibility for two cell lines

    International Nuclear Information System (INIS)

    This work focuses on one of the most exciting application areas of conjugated conducting polymers, which is cell culture and tissue engineering. To improve the biocompatibility of conducting polymers we present an easy method that involves the modification of the polymer backbone using L-cysteine. In this publication, we show the synthesis of polyaniline (PANI) films supported onto Polyethylene terephthalate (PET) films, and modified using cysteine (PANI-Cys) in order to generate a biocompatible substrate for cell culture. The PANI-Cys films are characterized by Fourier Transform infrared and UV–visible spectroscopy. The changes in the hydrophilicity of the polymer films after and before the modification were tested using contact angle measurements. After modification the contact angle changes from 86° ± 1 to 90° ± 1, suggesting a more hydrophylic surface. The adhesion properties of LM2 and HaCaT cell lines on the surface of PANI-Cys films in comparison with tissue culture plastic (TCP) are studied. The PANI-Cys film shows better biocompatibility than PANI film for both cell lines. The cell morphologies on the TCP and PANI-Cys film were examined by florescence and Atomic Force Microscopy (AFM). Microscopic observations show normal cellular behavior when PANI-Cys is used as a substrate of both cell lines (HaCaT and LM2) as when they are cultured on TCP. The ability of these PANI-Cys films to support cell attachment and growth indicates their potential use as biocompatible surfaces and in tissue engineering. - Highlights: • A new surface PANI-Cys was produced on films of polyethylene terephthalate. • The relationship between surface characteristics and biocompatibility is analyzed. • The PANI-Cys film presents good biocompatibility for two cell lines

  14. Cysteine modified polyaniline films improve biocompatibility for two cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Yslas, Edith I., E-mail: eyslas@exa.unrc.edu.ar [Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Agencia Postal Nro3, X580BYA Río Cuarto (Argentina); Cavallo, Pablo; Acevedo, Diego F.; Barbero, César A. [Departamento de Química, Universidad Nacional de Río Cuarto, Agencia Postal Nro3, X580BYA Río Cuarto (Argentina); Rivarola, Viviana A. [Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Agencia Postal Nro3, X580BYA Río Cuarto (Argentina)

    2015-06-01

    This work focuses on one of the most exciting application areas of conjugated conducting polymers, which is cell culture and tissue engineering. To improve the biocompatibility of conducting polymers we present an easy method that involves the modification of the polymer backbone using L-cysteine. In this publication, we show the synthesis of polyaniline (PANI) films supported onto Polyethylene terephthalate (PET) films, and modified using cysteine (PANI-Cys) in order to generate a biocompatible substrate for cell culture. The PANI-Cys films are characterized by Fourier Transform infrared and UV–visible spectroscopy. The changes in the hydrophilicity of the polymer films after and before the modification were tested using contact angle measurements. After modification the contact angle changes from 86° ± 1 to 90° ± 1, suggesting a more hydrophylic surface. The adhesion properties of LM2 and HaCaT cell lines on the surface of PANI-Cys films in comparison with tissue culture plastic (TCP) are studied. The PANI-Cys film shows better biocompatibility than PANI film for both cell lines. The cell morphologies on the TCP and PANI-Cys film were examined by florescence and Atomic Force Microscopy (AFM). Microscopic observations show normal cellular behavior when PANI-Cys is used as a substrate of both cell lines (HaCaT and LM2) as when they are cultured on TCP. The ability of these PANI-Cys films to support cell attachment and growth indicates their potential use as biocompatible surfaces and in tissue engineering. - Highlights: • A new surface PANI-Cys was produced on films of polyethylene terephthalate. • The relationship between surface characteristics and biocompatibility is analyzed. • The PANI-Cys film presents good biocompatibility for two cell lines.

  15. Biocompatible Metal-Oxide Nanoparticles: Nanotechnology Improvement of Conventional Prosthetic Acrylic Resins

    International Nuclear Information System (INIS)

    Nowadays, most products for dental restoration are produced from acrylic resins based on heat-cured Poly(Methyl Methacrylate) (PMMA). The addition of metal nanoparticles to organic materials is known to increase the surface hydrophobicity and to reduce adherence to biomolecules. This paper describes the use of nano structured materials, TiO2 and Fe2O3, for simultaneously coloring and/or improving the antimicrobial properties of PMMA resins. Nanoparticles of metal oxides were included during suspension polymerization to produce hybrid metal oxides-alginate-containing PMMA. Metal oxide nanoparticles were characterized by dynamic light scattering, and X-ray diffraction. Physicochemical characterization of synthesized resins was assessed by a combination of spectroscopy, scanning electron microscopy, viscometry, porosity, and mechanical tests. Adherence of Candida albicans cells and cellular compatibility assays were performed to explore biocompatibility and microbial adhesion of standard and novel materials. Our results show that introduction of biocompatible metal nanoparticles is a suitable means for the improvement of conventional acrylic dental resins.

  16. Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane.

    Science.gov (United States)

    Wang, Kai; Hou, Wen-Da; Wang, Xi; Han, Chengsheng; Vuletic, Ivan; Su, Ni; Zhang, Wen-Xi; Ren, Qiu-Shi; Chen, Liangyi; Luo, Ying

    2016-09-01

    Implantable immunoisolation membranes need to possess superior biocompatibility to prohibit the fibrotic deposition that would reduce the nutrient supply and impair the viability/function of the encapsulated cells. Here, electrospun membranes based on thermoplastic polyurethane (TPU) were fabricated to contain microfibers (PU-micro) or nanofibers (PU-nano). The two types of membranes were compared in terms of their interaction with macrophage cells and the host tissues. It was found that the fibrous membranes of different topographies possess distinct material properties: PU-nano caused minimal macrophage responses in vitro and in vivo and induced only mild foreign body reactions compared to PU-micro membranes. A flat macroencapsulation device was fabricated using PU-nano membranes and its immunoisolation function investigated in subcutaneous transplantation models. The nanofibrous device demonstrated the capability to effectively shield the allografts from the immune attack of the host. Nanotopography may confer biocompatibility to materials and nanofibrous materials warrant further study for development of "invisible" immunoisolation devices for cell transplantation. PMID:27344368

  17. Biocompatibility Studies on Fibrin Glue Cultured with Bone Marrow Mesenchymal Stem Cells in Vitro

    Institute of Scientific and Technical Information of China (English)

    方煌; 彭松林; 陈安民; 黎逢峰; 任凯; 胡宁

    2004-01-01

    Summary: By culturing bone marrow mesenchymal stem cells of rabbits with fibrin glue in vitro,the biocompatibility of fibrin glue was investigated to study whether this material can be used as scaffolds in bone tissue engineering. After 2-months old New Zealand rabbits had been anesthetized, about 4-6 ml of bone marrow were aspirated from rabbit femoral trochanter. The monocytes suspension was aspirated after bone marrow was centrifuged with lymphocyte separating medium and cultured primarily. Then the cells were divided into two groups: one was cultured with complete medium and the other with induced medium. The cells of the two groups were collected and inoculated to the culture plate containing fibrin glue. In the control group, cells were inoculated without fibrin glue. The implanted cells and materials were observed at different stages under a phase-contrast microscope and scanning electron microscope. MTT and alkaline phosphatase (ALP) were measured. Bone marrow mesenchymal stem cells grew on the surface of fibrin glue and adhered to it gradually. Cells light absorption value (A value) and the ALP content showed no significant difference. Fibrin glue had no inhibitory effect on cell morphology, growth, proliferation and differentiation. It has good biocompatibility and can be used as scaffold materials for bone marrow mesenchymal stem cells in bone tissue engineering.

  18. Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/polyglycolic acid copolymers.

    Science.gov (United States)

    Athanasiou, K A; Niederauer, G G; Agrawal, C M

    1996-01-01

    This is a review of salient studies of sterilization, toxicity, biocompatibility, clinical applications and current work in the field of orthopaedics, using implants made of polylactic acid (PLA), polyglycolic acid (PGA) and their copolymers. The intrinsic nature of these biomaterials renders them suitable for applications where temporally slow releases of bioactive agents in situ may be required. They are also desirable as fixation devices of bone, because they can virtually eliminate osteopenia associated with stress shielding or additional surgery. The majority of currently available sterilization techniques are not suitable for these thermoplastic materials and it may be desirable to develop new sterilization standards, which can account for the special character of PLA-PGA materials. Biocompatibility and toxicity studies suggest that, overall, PLA-PGA biomaterials may be suitable for orthopaedic applications, although certain problems, especially pertaining to reduction in cell proliferation, have been reported. Clinical applications are also promising, albeit not without problems usually associated with transient tissue inflammation. The future of these materials appears bright, especially in soft tissues. They may be used to address the exceedingly complex problem of osteochondral repair, but also as a means to enhance fixation and repair processes in tendons and ligaments. PMID:8624401

  19. Interaction of derived polymers from pyrrole with biocompatible solutions

    International Nuclear Information System (INIS)

    This work presents a study about the synthesis by plasma, the electric properties and superficial interaction of polymers derived from pyrrole doped with Iodine with potential use as bio material. Poly-pyrrole is a semiconductor and biocompatible polymer with potential application in the development of artificial muscles and implants where the electric interaction between cells and material is an important variable. The syntheses were made at 13.5 MHz in a glass tubular reactor of 1500 cm3 with electrodes of 6.5 cm diameter and stainless steel flanges. An electrode was connected to the RF terminal of the power supply that is combined with a matching coupling resistance. The monomer and dopant used in this work were pyrrole and Iodine respectively, in closed containers. They were vaporized and injected separately into the reactor at room temperature and 0.1 mbar. The vapors of the reagents mixed freely in the reactor. The synthesis time was 240 min at 40, 60, 80 and 100 W. The polymers were obtained as thin films adhered to the reactor walls. The films were washed and swollen with distilled water and removed from the reactor walls with a small spatula. The polymers were irradiated with gamma rays at 18 and 22 KGy. Due to the fact that the doses are cumulative, the final dose applied was 40 KGy. The polymers characterization was carried out by Fourier Transform Infrared Spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy, contact angle, electrical conductivity and X-ray diffraction. The analyses indicates that the polymers have very similar structure in almost the entire power range, showing C-O, C=C, C-H, O-H, N-H bonds with a predominantly amorphous structure. The TGA analyses showed that the material has 4 or 5 loses of material. The first one starts after that 115 C except for the material irradiated at 40 KGy, this one begins in 87 C, the second one is in the interval of 196 and 295 C, the third one between 311 and 500 C, and the last

  20. Biocompatible fluorescence-enhanced ZrO2-CdTe quantum dot nanocomposite for in vitro cell imaging

    Science.gov (United States)

    Lu, Zhisong; Zhu, Zhihong; Zheng, Xinting; Qiao, Yan; Guo, Jun; Li, Chang Ming

    2011-04-01

    With advances of quantum dots (QDs) in bioimaging applications, various materials have been used to coat QDs to reduce their nanotoxicity; however, the coating could introduce new toxic sources and quench the fluorescence in bioimaging applications. In this work, ZrO2, an excellent ceramic material with low extinction coefficient and good biocompatibility, is utilized to coat CdTe QDs for the first time. Experimental results show that ZrO2-QD nanocomposites with the size of ~ 30 nm possess enhanced fluorescence emission, lower nanotoxicity and gradually increased fluorescence under 350 nm light illumination. After functionalization with folic acid, they were applied to label cultured HeLa cells effectively. Therefore, the ZrO2-QD nanocomposites could be promising biocompatible nanomaterials with strong fluorescence emission to replace or complement QDs in biomedical applications.

  1. Biocompatible fluorescence-enhanced ZrO2-CdTe quantum dot nanocomposite for in vitro cell imaging

    International Nuclear Information System (INIS)

    With advances of quantum dots (QDs) in bioimaging applications, various materials have been used to coat QDs to reduce their nanotoxicity; however, the coating could introduce new toxic sources and quench the fluorescence in bioimaging applications. In this work, ZrO2, an excellent ceramic material with low extinction coefficient and good biocompatibility, is utilized to coat CdTe QDs for the first time. Experimental results show that ZrO2-QD nanocomposites with the size of ∼ 30 nm possess enhanced fluorescence emission, lower nanotoxicity and gradually increased fluorescence under 350 nm light illumination. After functionalization with folic acid, they were applied to label cultured HeLa cells effectively. Therefore, the ZrO2-QD nanocomposites could be promising biocompatible nanomaterials with strong fluorescence emission to replace or complement QDs in biomedical applications.

  2. Biocompatibility assessment of pastes containing Copaiba oilresin, propolis, and calcium hydroxide in the subcutaneous tissue of rats

    OpenAIRE

    Lucas Garcia; Santos Cristiane; Mestriner Jr. Wilson; Mestriner Jr. Soraya; Lopes A Ruberval; Ramos Mônica; de Freitas Osvaldo

    2011-01-01

    Aim : To assess the biocompatibility of two endodontic pastes based on calcium hydroxide and propolis, using two vehicles - non-fractionated Copaiba-oilresin (A) and volatile fraction of Copaiba-oilresin (B), in the connective tissue of rats. Materials and Methods : Fifteen rats had four polyethylene tubes implanted in their backs; each pair of tubes contained one of the pastes. The tube side was considered the control. After 7, 21, and 42 days, the animals were euthanized. Results : ...

  3. Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

    OpenAIRE

    Jantzen, Alexandra E.; Lane, Whitney O.; Gage, Shawn M.; Haseltine, Justin M; Galinat, Lauren J; Jamiolkowski, Ryan M.; Lin, Fu-Hsiung; Truskey, George A.; Achneck, Hardean E.

    2011-01-01

    Implantable cardiovascular devices are manufactured from artificial materials (e.g. titanium (Ti), expanded polytetrafluoroethylene), which pose the risk of thromboemboli formation1,2,3. We have developed a method to line the inside surface of Ti tubes with autologous blood-derived human or porcine endothelial progenitor cells (EPCs)4. By implanting Ti tubes containing a confluent layer of porcine EPCs in the inferior vena cava (IVC) of pigs, we tested the improved biocompatibility of the cel...

  4. Improved antibacterial activity and biocompatibility on vancomycin-loaded TiO2 nanotubes: in vivo and in vitro studies

    OpenAIRE

    Zhang, Hangzhou

    2013-01-01

    Hangzhou Zhang,1 Yu Sun,1 Ang Tian,2 Xiang Xin Xue,2 Lin Wang,1 Ali Alquhali,1 Xizhuang Bai1 1Department of Sports Medicine and Joint Surgery, First Affiliated Hospital of China Medical University, 2School of Materials and Metallurgy, Northeastern University, Shenyang, People's Republic of China Abstract: The goal for current orthopedic implant research is to design implants that have not only good biocompatibility but also antibacterial properties. TiO2 nanotubes (NTs) were fabricat...

  5. Improved antibacterial activity and biocompatibility on vancomycin-loaded TiO2 nanotubes: in vivo and in vitro studies

    OpenAIRE

    Zhang HZ; Sun Y; Tian A; Xue XX; Wang L; Alquhali A; Bai XZ

    2013-01-01

    Hangzhou Zhang,1 Yu Sun,1 Ang Tian,2 Xiang Xin Xue,2 Lin Wang,1 Ali Alquhali,1 Xizhuang Bai1 1Department of Sports Medicine and Joint Surgery, First Affiliated Hospital of China Medical University, 2School of Materials and Metallurgy, Northeastern University, Shenyang, People's Republic of China Abstract: The goal for current orthopedic implant research is to design implants that have not only good biocompatibility but also antibacterial properties. TiO2 nanotubes (NTs) were fabricated on...

  6. Interaction of derived polymers from pyrrole with biocompatible solutions; Interaccion de polimeros derivados de pirrol con soluciones biocompatibles

    Energy Technology Data Exchange (ETDEWEB)

    Lopez G, O. G.

    2010-07-01

    This work presents a study about the synthesis by plasma, the electric properties and superficial interaction of polymers derived from pyrrole doped with Iodine with potential use as bio material. Poly-pyrrole is a semiconductor and biocompatible polymer with potential application in the development of artificial muscles and implants where the electric interaction between cells and material is an important variable. The syntheses were made at 13.5 MHz in a glass tubular reactor of 1500 cm{sup 3} with electrodes of 6.5 cm diameter and stainless steel flanges. An electrode was connected to the RF terminal of the power supply that is combined with a matching coupling resistance. The monomer and dopant used in this work were pyrrole and Iodine respectively, in closed containers. They were vaporized and injected separately into the reactor at room temperature and 0.1 mbar. The vapors of the reagents mixed freely in the reactor. The synthesis time was 240 min at 40, 60, 80 and 100 W. The polymers were obtained as thin films adhered to the reactor walls. The films were washed and swollen with distilled water and removed from the reactor walls with a small spatula. The polymers were irradiated with gamma rays at 18 and 22 KGy. Due to the fact that the doses are cumulative, the final dose applied was 40 KGy. The polymers characterization was carried out by Fourier Transform Infrared Spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy, contact angle, electrical conductivity and X-ray diffraction. The analyses indicates that the polymers have very similar structure in almost the entire power range, showing C-O, C=C, C-H, O-H, N-H bonds with a predominantly amorphous structure. The TGA analyses showed that the material has 4 or 5 loses of material. The first one starts after that 115 C except for the material irradiated at 40 KGy, this one begins in 87 C, the second one is in the interval of 196 and 295 C, the third one between 311 and 500 C, and the

  7. Blends of thermoplastic polyurethane and polydimethylsiloxane rubber: assessment of biocompatibility and suture holding strength of membranes.

    Science.gov (United States)

    Rajan, Krishna Prasad; Al-Ghamdi, Ahmed; Parameswar, Ramesh; Nando, G B

    2013-01-01

    In the present investigation, a compatibilized blend of thermoplastic polyurethane (TPU) and polydimethylsiloxane (PDMS) is prepared by using copolymer of ethylene and methyl acrylate (EMA) as a reactive compatibilizer. Detailed in vitro biocompatibility studies were carried out for this compatibilized blend and the material was found noncytotoxic towards L929 mouse fibroblast subcutaneous connective tissue cell line. Microporosity was created on the surface of membranes prepared from the blend material by adopting the crazing mechanism. Cell proliferation and growth studies on the membranes surface showed that the microporous surface favoured ingrowth of the cells compared with a nonmicroporous surface. Suture holding strength studies indicate that the microporous membranes have enough strength to withstand the cutting and tearing forces through the suture hole. This blend material could be evaluated further to find its suitability in various implant applications. PMID:24454376

  8. 不同牙科修复材料生物相容性及3种材料充填恒磨牙邻面龋的临床分析%Clinical analysis of biocompatibility of different dental restorative materials and 3 kinds of materials to ifll the proximal dental caries of accessional teeth

    Institute of Scientific and Technical Information of China (English)

    马海英

    2016-01-01

    目的:研究并分析不同牙科修复材料充填恒磨牙邻面龋的临床效果。方法回顾性分析2010年5月至2012年5月收治的444例恒磨牙邻面龋患者的临床资料。按照充填材料不同将入选患者分为A组(玻璃离子水门汀充填)、B组(光固化复合树脂充填)、C组(银汞合金充填),分析不同牙科修复材料对患牙的修复成功率。结果修复后1年和3年复诊,A组患牙修复成功率明显低于B组和C组(P<0.05)。修复后1年,B组与C组患牙修复成功率比较差异无显著性(P>0.05);修复后3年,B组患牙修复成功率高于C组(P<0.05)。结论光固化复合树脂有助于提高恒磨牙邻面龋的修复成功率,值得临床推广。%ObjectiveTo analyze the clinical effect of different dental restorative materials on the proximal dental caries of accessional teeth.MethodA retrospective analysis of clinical data of 444 patients with proximal dental caries of accessional teeth treated in our hospital from May 2010 to May 2012 was performed, according to the different iflling materials, the patients were divided into group A (glass-ionomer cement), group B (light-cured composite resin) and group C (silver amalgam), analyzed the success rate of different dental restorative materials.ResultFollowed-up 1 and 3 years respectively, the success rate of group A was signiifcantly lower than that of group B and group C (P0.05); after 3 years of restoration, the success rate of group B was higher than group C (P<0.05).ConclusionLight-cured composite resin is helpful to improve the success rate of proximal dental caries of accessional teeth, and it is worth to be popularized in clinic.

  9. Biocompatibility studies of polyacrylonitrile membranes modified with carboxylated polyetherimide

    Energy Technology Data Exchange (ETDEWEB)

    Senthilkumar, S.; Rajesh, S.; Jayalakshmi, A.; Mohan, D., E-mail: mohantarun@gmail.com

    2013-10-15

    Poly (ether-imide) (PEI) was carboxylated and used as the hydrophilic modification agent for the preparation of polyacrylonitrile (PAN) membranes. Membranes were prepared with different blend compositions of PAN and CPEI by diffusion induced precipitation. The modified membranes were characterized by thermo gravimetric analysis (TGA), mechanical analysis, scanning electron microscopy (SEM) and contact angle measurement to understand the influence of CPEI on the properties of the membranes. The biocompatibility studies exhibited reduced plasma protein adsorption, platelet adhesion and thrombus formation on the modified membrane surface. The complete blood count (CBC) results of CPEI incorporated membranes showed stable CBC values and significant decrease in the complement activation were also observed. In addition to good cytocompatibility, monocytes cultured on these modified membranes exhibited improved functional profiles in 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Thus it could be concluded that PAN/CPEI membranes with excellent biocompatibility can be useful for hemodialysis. Highlights: • Carboxylated PEI was prepared and utilized as hydrophilic modification agent. • CPEI incorporated into PAN to improved biocompatibility and cyto compatibility • Biocompatibility of membranes was correlated with morphology and hydrophilicity. • Antifouling studies of the PAN/CPEI membranes was studied by BSA as model foulant.

  10. Biocompatibility of phosphorylcholine coated stents in normal porcine coronary arteries

    NARCIS (Netherlands)

    D.M. Whelan (Deirdre); S.C. Krabbendam; P.D. Verdouw (Pieter); P.W.J.C. Serruys (Patrick); E.A. van Vliet (Erwin); W.J. van der Giessen (Wim); H.M.M. van Beusekom (Heleen)

    2000-01-01

    textabstractOBJECTIVE: To improve the biocompatibility of stents using a phosphorylcholine coated stent as a form of biomimicry. INTERVENTIONS: Implantation of phosphorylcholine coated (n = 20) and non-coated (n = 21) stents was performed in the coronary arteries of 25 pigs. The an

  11. Synthesis and characterization of biocompatible hydroxyapatite coated ferrite

    Indian Academy of Sciences (India)

    S Deb; J Giri; S Dasgupta; D Datta; D Bahadur

    2003-12-01

    Ferrite particles coated with biocompatible phases can be used for hyperthermia treatment of cancer. We have synthesized substituted calcium hexaferrite, which is not stable on its own but is stabilized with small substitution of La. Hexaferrite of chemical composition (CaO)0.75(La2O3)0.20(Fe2O3)6 was prepared using citrate gel method. Hydroxyapatite was prepared by precipitating it from aqueous solution of Ca(NO3)2 and (NH4)2HPO4 maintaining pH above 11. Four different methods were used for coating of hydroxyapatite on ferrite particles. SEM with EDX and X-ray diffraction analysis shows clear evidence of coating of hydroxy-apatite on ferrite particles. These coated ferrite particles exhibited coercive field up to 2 kOe, which could be made useful for hysteresis heating in hyperthermia. Studies by culturing BHK-21 cells and WBC over the samples show evidence of biocompatibility. SEM micrographs and cell counts give clear indication of cell growth on the surface of the sample. Finally coated ferrite particle was implanted in Kasaulli mouse to test its biocompatibility. The magnetic properties and biocompatibility studies show that these hydroxyapatite coated ferrites could be useful for hyperthermia.

  12. Biocompatibility studies of polyacrylonitrile membranes modified with carboxylated polyetherimide

    International Nuclear Information System (INIS)

    Poly (ether-imide) (PEI) was carboxylated and used as the hydrophilic modification agent for the preparation of polyacrylonitrile (PAN) membranes. Membranes were prepared with different blend compositions of PAN and CPEI by diffusion induced precipitation. The modified membranes were characterized by thermo gravimetric analysis (TGA), mechanical analysis, scanning electron microscopy (SEM) and contact angle measurement to understand the influence of CPEI on the properties of the membranes. The biocompatibility studies exhibited reduced plasma protein adsorption, platelet adhesion and thrombus formation on the modified membrane surface. The complete blood count (CBC) results of CPEI incorporated membranes showed stable CBC values and significant decrease in the complement activation were also observed. In addition to good cytocompatibility, monocytes cultured on these modified membranes exhibited improved functional profiles in 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Thus it could be concluded that PAN/CPEI membranes with excellent biocompatibility can be useful for hemodialysis. Highlights: • Carboxylated PEI was prepared and utilized as hydrophilic modification agent. • CPEI incorporated into PAN to improved biocompatibility and cyto compatibility • Biocompatibility of membranes was correlated with morphology and hydrophilicity. • Antifouling studies of the PAN/CPEI membranes was studied by BSA as model foulant

  13. Biocompatibility of mineral trioxide aggregate and three new endodontic cements: An animal study

    Directory of Open Access Journals (Sweden)

    Mohammad-Ghasem Aminozarbian

    2012-01-01

    Conclusion: According to the results of the current study, biocompatibility of CAAC and WOLCA cement were comparable with that of MTA, but CAAC Plus induced an inflammatory response higher than MTA, therefore is not biocompatible.

  14. Nanocrystalline β-Ti alloy with high hardness, low Young's modulus and excellent in vitro biocompatibility for biomedical applications

    International Nuclear Information System (INIS)

    High strength, low Young's modulus and good biocompatibility are desirable but difficult to simultaneously achieve in metallic implant materials for load bearing applications, and these impose significant challenges in material design. Here we report that a nano-grained β-Ti alloy prepared by high-pressure torsion exhibits remarkable mechanical and biological properties. The hardness and modulus of the nano-grained Ti alloy were respectively 23% higher and 34% lower than those of its coarse-grained counterpart. Fibroblast cell attachment and proliferation were enhanced, demonstrating good in vitro biocompatibility of the nano-grained Ti alloy, consistent with demonstrated increased nano-roughness on the nano-grained Ti alloy. Results suggest that the nano-grained β-Ti alloy may have significant application as an implant material in dental and orthopedic applications. - Highlights: • A bulk nanocrystalline β-Ti alloy was produced by high-pressure torsion processing. • Excellent mechanical properties for biomedical implants were obtained. • Enhanced in vitro biocompatibility was also demonstrated

  15. Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells

    Directory of Open Access Journals (Sweden)

    Leticia Boldrin MESTIERI

    2015-10-01

    Full Text Available Mineral Trioxide Aggregate (MTA is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs.Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP; MTA Fillapex (MTAF and FillCanal (FC. Biocompatibility was evaluated with MTT and Neutral Red (NR assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4. Unexposed cells were the positive control (CT. Bioactivity was assessed by alkaline phosphatase (ALP enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution. All data were analyzed by ANOVA and Tukey post-test (p≤0.05%.Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%. Cells exposed to MTAF and FC (1:2 and 1:4 dilutions showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro. MTAP may be considered a promising material for endodontic treatments.

  16. Biocompatibility and Toxicity of Poly(vinyl alcohol/N,O-Carboxymethyl Chitosan Scaffold

    Directory of Open Access Journals (Sweden)

    Tunku Kamarul

    2014-01-01

    Full Text Available The in vivo biocompatibility and toxicity of PVA/NOCC scaffold were tested by comparing them with those of a biocompatible inert material HAM in a rat model. On Day 5, changes in the blood parameters of the PVA/NOCC-implanted rats were significantly higher than those of the control. The levels of potassium, creatinine, total protein, A/G, hemoglobulin, erythrocytes, WBC, and platelets were not significantly altered in the HAM-implanted rats, when compared with those in the control. On Day 10, an increase in potassium, urea, and GGT levels and a decrease in ALP, platelet, and eosinophil levels were noted in the PVA/NOCC-implanted rats, when compared with control. These changes were almost similar to those noted in the HAM-implanted rats, except for the unaltered potassium and increased neutrophil levels. On Day 15, the total protein, A/G, lymphocyte, monocyte, and eosinophil levels remained unaltered in the PVA/NOCC-implanted rats, whereas urea, A/G, WBC, lymphocyte, and monocyte levels remained unchanged in the HAM-implanted rats. Histology and immunohistochemistry analyses revealed inflammatory infiltration in the PVA/NOCC-implanted rats, but not in the HAM-implanted rats. Although a low toxic tissue response was observed in the PVA/NOCC-implanted rats, further studies are necessary to justify the use of this material in tissue engineering applications.

  17. Susceptibility to corrosion and in vitro biocompatibility of a laser-welded composite orthodontic arch wire.

    Science.gov (United States)

    Zhang, Chao; Sun, Xinhua; Zhao, Shuang; Yu, Wenwen; Sun, Daqian

    2014-01-01

    Composite arch-wire (CoAW) is an arch wire formed by solder connection of nickel titanium shape memory alloy and stainless steel wire. The purpose of the present study was to investigate the biocompatibility of CoAW as an important foundation for its clinical application. The electrochemical corrosion and ion release behavior of CoAW upon immersion in solutions simulating oral cavity conditions were measured to evaluate the corrosion behavior of CoAW. Murine L-929 cells were co-cultured with CoAW extract to evaluate the cytotoxicity of the corrosion products in vitro. Polarization tests indicated that CoAW is resistant to corrosion in the tested artificial saliva (AS)-based solutions (chloric solution, simple AS, fluorinated AS, and protein-containing AS), and the amount of toxic copper ions released after immersion was lower than average daily dietary intake levels. The cytotoxicity experiments demonstrated the in vitro biocompatibility of CoAW. Based on the combined advantages of its base materials CoAW, with its resistance to biocorrosion and in vitro cytocompatibility, is a promising alternative material for use in orthodontic fixation applications. PMID:23918081

  18. The biocompatibility of silk fibroin and acellular collagen scaffolds for tissue engineering in the ear

    International Nuclear Information System (INIS)

    Recent experimental studies have shown the suitability of silk fibroin scaffold (SFS) and porcine-derived acellular collagen I/III scaffold (ACS) as onlay graft materials for tympanic membrane perforation repair. The aims of this study were to further characterize and evaluate the in vivo biocompatibility of SFS and ACS compared with commonly used materials such as Gelfoam and paper in a rat model. The scaffolds were implanted in subcutaneous (SC) tissue and middle ear (ME) cavity followed by histological and otoscopic evaluation for up to 26 weeks. Our results revealed that SFS and ACS were well tolerated and compatible in rat SC and ME tissues throughout the study. The tissue response adjacent to the implants evaluated by histology and otoscopy showed SFS and ACS to have a milder tissue response with minimal inflammation compared to that of paper. Gelfoam gave similar results to SFS and ACS after SC implantation, but it was found to be associated with pronounced fibrosis and osteoneogenesis after ME implantation. It is concluded that SFS and ACS both were biocompatible and could serve as potential alternative scaffolds for tissue engineering in the ear. (paper)

  19. Effect of biocompatibility of different dental restorative materials and 3 kinds of materials on the surface caries of permanent molars%不同口腔修复材料生物相容性及3种材料充填恒磨牙邻面龋的疗效观察

    Institute of Scientific and Technical Information of China (English)

    唐梦柏

    2015-01-01

    目的对比分析在恒磨牙邻面龋填充中应用3种不同的口腔修复材料的疗效。方法选取2011年6月~2012年6月我院收治的恒磨牙邻面龋患者150例作为研究对象,依据所使用口腔修复材料的不同,将其分为甲、乙、丙三组,各50例。治疗中,甲组使用银汞合金,乙组使用玻璃离子水门汀,丙组使用光固化复合树脂。结果治疗1年后,甲、丙两组的有效率比较,差异无统计学意义(P>0.05),但均明显高于乙组,差异有统计学意义(P<0.05);治疗3年后,甲组的有效率显著高于丙组,丙组的有效率显著高于乙组,差异有统计学意义(P<0.05)。结论在口腔修复治疗中,银汞合金的修复效果显著,值得临床推广。%ObjectiveTo comparison and analysis on permanent molar caries fi lling in the application of three different specifi c effect of oral repair material.MethodsChoose 150 cases of permanent molar caries patients, on the basis of the use of oral repair material is different in its list of a, b, c three groups, each group of 50 cases, treatment, students' use of silver amalgam, use glass ion cement b, c group with light-cured composite resin.Results1 year after treatment, two groups of efficient comparison: a, c, there was no statistically significant difference (P>0.05), but were significantly higher than group b, the difference had statistical significance (P<0.05); Treatment after three years, students' effective to significantly higher than that of c group, effi cient is signifi cantly higher than group b, c group differences were statistically signifi cant (P<0.05). ConclusionIn the oral cavity repair treatment, silver amalgam restoration effect is good, can be promoted.

  20. Preparation and characterization of bio-compatible Fe3O4-Polydopamine spheres with core/shell nanostructure

    International Nuclear Information System (INIS)

    Graphical abstract: Bio-compatible and superparamagnetic Fe3O4-Polydopamine nanocomposites with well-defined core/shell nanostructures have been successfully synthesized by using an in situ self-polymerization method. These unique core/shell materials could be applied in catalyst supports or drug delivery. -Highlights: → A facile in situ self-polymerization method was successfully developed to synthesize bio-compatible and superparamagnetic Fe3O4-Polydopamine (PDA) nanocomposites with well-defined core/shell nanostructures. → The size of the core/shell product can be controlled by varying the size of the central Fe3O4 core, and different thicknesses of the PDA shells are obtained by tuning the dopamine monomer concentration. → To the best of our knowledge, this is first report focused on the controllable synthesis of core/shell Fe3O4-PDA microspheres and the investigation of its cell viability. → These unique core/shell materials would be applied in catalyst supports or drug delivery. - Abstract: Bio-compatible and superparamagnetic Fe3O4-Polydopamine nanocomposites with well-defined core/shell nanostructures have been successfully synthesized by using an in situ self-polymerization method. The size of the core/shell product can be controlled by varying the size of the central Fe3O4 core, and different thicknesses of the PDA shells are obtained by tuning the dopamine monomer concentration. The morphology, phase composition and crystallinity of the as-prepared nanocomposites have been characterized by transmission electron microscopy (TEM) and powder X-ray diffraction (XRD). The 5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide (MTT) cytotoxic analysis shows that the product is biocompatible, with a low toxicity. These unique core/shell materials would be applied in catalyst supports or drug delivery.

  1. Preliminary study towards photoactivity enhancement using a biocompatible titanium dioxide/carbon nanotubes composite

    International Nuclear Information System (INIS)

    Graphical abstract: Scheme demonstrating the experimental steps toward the formation of titania/multiwalled carbon nanotubes (TiO2-MWCNTs) from multiwalled carbon nanotubes (MWCNT). - Highlights: • Easy and efficient method of impregnation carbon nanotubes with titania. • High photoactivity. • Correlation between the interaction of carbon nanotubes with titania on the photocatalytic properties. • High biocompatibility of the nanotubes. - Abstract: Recent research is focused on the enhancement in photoactivity of titanium dioxide/carbon nanotubes through formation of novel nanocomposites that exhibit a high specific surface area, remarkable electron transfer and biocompatibility. Here, we explore a new synthesis route in the system composed of nanocrystalline titanium dioxide supported on external walls and inner space of multiwalled carbon nanotubes (MWCNT). The advantages of this method are: its simplicity, direct fusion of titanium dioxide particles on the carbon material, and formation of chemical bond Ti–O–C between TiO2 and MWCNT. Photocatalytic performance of this system has been compared to a commercial catalyst (Degussa P25) in a model reaction of phenol decomposition in/under UV light. The efficiency of the process increased by the factor of 2.5 when the TiO2–MWCNT photocatalyst was utilized. Further, the photoactive nanocomposite was analysed towards its biocompatibility in order to establish a safe dose of the catalyst. Its influence on the cells viability was studied on mouse fibroblasts and human liver tissue cells, in the range from 0 to 100 μg/mL. This has revealed that the composite in concentrations up to 25 μg/mL exerted low toxicity, which allowed for finding a compromise between the highest safe dose and acceptable photoactivity of the catalyst

  2. Synthesis, characterization and biocompatibility evaluation of hydroxyapatite - gelatin polyLactic acid ternary nanocomposite

    Directory of Open Access Journals (Sweden)

    Z. Nabipour

    2016-04-01

    Full Text Available Objective(s: The current study reports the production and biocompatibility evaluation of a ternary nanocomposite consisting of HA, PLA, and gelatin for biomedical application.Materials and Methods: Hydroxyapatite nanopowder (HA: Ca10(PO46(OH2 was produced by burning the bovine cortical bone within the temperature range of 350-450 oC followed by heating in an oven at 800. Synthesis of the ternary nanocomposite was carried out in two steps: synthesis of gelatin-hydroxyapatite binary nanocomposite and addition of poly lactic acid with different percentages to the resulting composition. The crystal structure was determined by X-ray diffraction (XRD, while major elements and impurities of hydroxyapatite were identified by elemental analysis of X-ray fluorescence (XRF. Functional groups were determined by Fourier transform infrared spectroscopy (FTIR. Morphology and size of the nanocomposites were evaluated using field emission scanning electron microscope (FE-SEM.Biocompatibility of nanocomposites was investigated by MTT assay. Results: XRD patterns verified the ideal crystal structure of the hydroxyapatite, which indicated an appropriate synthesis process and absence of disturbing phases. Results of FTIR analysis determined the polymers’ functional groups, specified formation of the polymers on the hydroxyapatite surface, and verified synthesis of nHA/PLA/Gel composite. FESEM images also indicated the homogeneous structure of the composite in the range of 50 nanometers. MTT assay results confirmed the biocompatibility of nanocomposite samples.Conclusion: This study suggested that the ternary nanocomposite of nHA/PLA/Gel can be a good candidate for biomedical application such as drug delivery systems, but for evaluation of its potential in hard tissue replacement, mechanical tests should be performed.

  3. Characterization, antimicrobial activities, and biocompatibility of organically modified clays and their nanocomposites with polyurethane.

    Science.gov (United States)

    Wang, Ming-Chien; Lin, Jiang-Jen; Tseng, Hsiang-Jung; Hsu, Shan-hui

    2012-01-01

    A novel method to exfoliate the montmorillonite clay was developed previously to generate random nanosilicate platelets (NSP), one kind of delaminated clay. To improve their dispersion in a polymer, we modified NSPs by three types of surfactants (cationic Qa, nonionic Qb, and anionic Qc) in this study and used them to prepare nanocomposites with polyurethane (PU). The zeta potential, antimicrobial ability, and biocompatibility of these surfactant-modified NSPs (abbreviated "NSQ") were characterized. It was found that the zeta potential of Qa-modified NSP (NSQa) was positive, whereas those of NSP and the other two NSQs (NSQb and NSQc) were negative. All NSQ presented less cytotoxicity than NSP. NSQa and NSQc showed excellent antimicrobial activities against S. aureus (Gram-positive strain) and E. coli (Gram-negative strain). The nanocomposites of NSQ with PU were then characterized for surface and mechanical properties, cell attachment and proliferation, antimicrobial activity in vitro, and biocompatibility in vivo. A higher surfactant to NSP ratio was found to improve the dispersion of NSQ in PU matrix. The mechanical properties of all PU/NSQ nanocomposites were significantly enhanced. Among various NSQ, only NSQa were observed to migrate to the composite surface. The attachment and proliferation of endothelial cells and fibroblasts in vitro as well as biocompatibility in vivo were significantly better for PU/NSQa containing 1% of NSQa than other materials. The microbiostasis ratios of PU/NSQ nanocomposites containing 1% NSQa or NSQc were >90%. These results proposed the safety and potential antimicrobial applications of surfactant-modified delaminated clays and their nanocomposites with PU polymer. PMID:22128903

  4. Biomimetic synthesis and biocompatibility evaluation of carbonated apatites template-mediated by heparin

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Yi [Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081 (China); Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Sun, Yuhua [Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081 (China); Chen, Xiaofang [Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Zhu, Peizhi, E-mail: pzzhu@umich.edu [Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China); Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055 (United States); Wei, Shicheng, E-mail: sc-wei@pku.edu.cn [Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081 (China); Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China)

    2013-07-01

    Biomimetic synthesis of carbonated apatites with good biocompatibility is a promising strategy for the broadening application of apatites for bone tissue engineering. Most researchers were interested in collagen or gelatin-based templates for synthesis of apatite minerals. Inspired by recent findings about the important role of polysaccharides in bone biomineralization, here we reported that heparin, a mucopolysaccharide, was used to synthesize carbonated apatites in vitro. The results indicated that the Ca/P ratio, carbon content, crystallinity and morphology of the apatites varied depending on the heparin concentration and the initial pH value. The morphology of apatite changed from flake-shaped to needle-shaped, and the degree of crystallinity decreased with the increasing of heparin concentration. Biocompatibility of the apatites was tested by proliferation and alkaline phosphatase activity of MC3T3-E1 cells. The results suggested that carbonated apatites synthesized in the presence of heparin were more favorable to the proliferation and differentiation of MC3T3-E1 cells compared with traditional method. In summary, the heparin concentration and the initial pH value play a key role in the chemical constitution and morphology, as well as biological properties of apatites. These biocompatible nano-apatite crystals hold great potential to be applied as bioactive materials for bone tissue engineering. - Highlights: • Heparin was used as a template to synthesize needle-shaped nano-apatite. • Changing the pH value and concentration led to different properties of apatite. • Apatite prepared by heparin was more favorable to the osteogenic differentiation. • Possible synthesis mechanism of apatite templated by heparin was described.

  5. An in vitro study on the biocompatibility of WE magnesium alloys.

    Science.gov (United States)

    Ge, Shuping; Wang, Yi; Tian, Jie; Lei, Daoxi; Yu, Qingsong; Wang, Guixue

    2016-04-01

    Magnesium alloys are being actively studied for intravascular stent applications because of their good mechanical strength and biocompatibility. To rule out the high allergenicity of nickel and neurotoxicity of aluminum element, four kinds of WE magnesium alloys (where "W" represents the metallic element Y and "E" represents mixed rare earth (RE) elements; Y: 2.5, 5.0, 6.5, and 7.5 wt %; Nd: 1.0, 2.6, 2.5, and 4.2 wt %; Zr: 0.8 wt %) were chosen for in vitro investigation of their biocompatibility using cell culture. The results showed that, with the increase of rare earth elements in WE magnesium alloys, fibrinogen adsorption decreased and coagulation function was improved. It was also found that WE magnesium alloys promoted the adhesion of endothelial cells. With the increase of adhesion time, adhered cell numbers increased gradually. With 25% extracts, all the WE alloys promoted cell migration, while 100% extracts were not conducive to cell migration. Based on the above results, WE magnesium alloys 5.0WE (5.0Y-2.6Nd-0.8Zr) and 6.5WE (6.5Y-2.5Nd-0.8Zr) have better biocompatibility as compared with that with 2.5WE (2.5Y-1.0Nd-0.8Zr) and 7.5WE (7.5Y-4.2Nd-0.8Zr), and could be as the promising candidate materials for medical stent applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 482-487, 2016. PMID:25939488

  6. Biomimetic synthesis and biocompatibility evaluation of carbonated apatites template-mediated by heparin

    International Nuclear Information System (INIS)

    Biomimetic synthesis of carbonated apatites with good biocompatibility is a promising strategy for the broadening application of apatites for bone tissue engineering. Most researchers were interested in collagen or gelatin-based templates for synthesis of apatite minerals. Inspired by recent findings about the important role of polysaccharides in bone biomineralization, here we reported that heparin, a mucopolysaccharide, was used to synthesize carbonated apatites in vitro. The results indicated that the Ca/P ratio, carbon content, crystallinity and morphology of the apatites varied depending on the heparin concentration and the initial pH value. The morphology of apatite changed from flake-shaped to needle-shaped, and the degree of crystallinity decreased with the increasing of heparin concentration. Biocompatibility of the apatites was tested by proliferation and alkaline phosphatase activity of MC3T3-E1 cells. The results suggested that carbonated apatites synthesized in the presence of heparin were more favorable to the proliferation and differentiation of MC3T3-E1 cells compared with traditional method. In summary, the heparin concentration and the initial pH value play a key role in the chemical constitution and morphology, as well as biological properties of apatites. These biocompatible nano-apatite crystals hold great potential to be applied as bioactive materials for bone tissue engineering. - Highlights: • Heparin was used as a template to synthesize needle-shaped nano-apatite. • Changing the pH value and concentration led to different properties of apatite. • Apatite prepared by heparin was more favorable to the osteogenic differentiation. • Possible synthesis mechanism of apatite templated by heparin was described

  7. Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model

    Directory of Open Access Journals (Sweden)

    Markel DC

    2012-01-01

    Full Text Available David C Markel1, S Trent Guthrie2, Bin Wu3, Zheng Song4, Paul H Wooley41Department of Orthopaedics, Providence Hospital and Medical Centers, Southfield, MI, 2Henry Ford Hospital, Detroit, MI, 3Department of Biomedical Engineering, Wayne State University, Detroit, MI, 4Orthopaedic Research Institute, Wichita, KS, USAAbstract: Bone grafting is utilized in nearly all orthopedic subspecialties and in most anatomic regions. Bone graft substitutes have the potential to offer similar efficacy as autogenous grafts without the morbidity of harvest. Several studies have noted the efficacy of new-generation bone substitute products, but few studies have evaluated their safety. This study characterizes and quantifies the inflammatory reaction to four different commercially available bone graft substitutes, which were examined using the in vivo murine air pouch biocompatibility model. One coralline hydroxyapatite product was chosen as an example of a purely osteoconductive material. Three demineralized bone matrix products were chosen to represent products that are both osteoconductive and osteoinductive. Samples were implanted in a murine air pouch and harvested after 14 days in situ. Pouch fluid was extracted, mRNA isolated, and reverse transcription polymerase chain reactions carried out to detect interleukin-1 gene expression as a marker for inflammation. In addition, multiple histological characteristics were examined to quantify cellular responses to the implanted materials. All bone graft substitutes induced a significant inflammatory response compared with negative controls. Histology and polymerase chain reaction data indicated that the level of inflammatory reaction was elevated in materials with a higher demineralized bone matrix to carrier proportion. The hydroxyapatite product generated a low inflammatory reaction. In conclusion, this study used an in vivo model of biocompatibility to demonstrate that a significant inflammatory reaction occurs

  8. Influence of Oxygen Content and Microstructure on the Mechanical Properties and Biocompatibility of Ti–15 wt%Mo Alloy Used for Biomedical Applications

    Directory of Open Access Journals (Sweden)

    José R. S. Martins, Jr.

    2014-01-01

    Full Text Available The Ti–15Mo alloy has its mechanical properties strongly altered by heat treatments and by addition of interstitial elements, such as, oxygen, for example. In this sense, the objective of this paper is to analyze the effect of the introduction of oxygen in selected mechanical properties and the biocompatibility of Ti–15Mo alloy. The samples used in this study were prepared by arc-melting and characterized by density measurements, X-ray diffraction, scanning electron microscopy, microhardness, modulus of elasticity, and biocompatibility tests. Hardness measurements were shown to be sensitive to concentration of oxygen. The modulus results showed interstitial influence in value; this was verified under several conditions to which the samples were exposed. Cytotoxicity tests conducted in vitro showed that the various processing conditions did not alter the biocompatibility of the material.

  9. Development and testing of new biologically-based polymers as advanced biocompatible contact lenses

    Energy Technology Data Exchange (ETDEWEB)

    Bertozzi, Carolyn R.

    2000-06-01

    Nature has evolved complex and elegant materials well suited to fulfill a myriad of functions. Lubricants, structural scaffolds and protective sheaths can all be found in nature, and these provide a rich source of inspiration for the rational design of materials for biomedical applications. Many biological materials are based in some fashion on hydrogels, the crosslinked polymers that absorb and hold water. Biological hydrogels contribute to processes as diverse as mineral nucleation during bone growth and protection and hydration of the cell surface. The carbohydrate layer that coats all living cells, often referred to as the glycocalyx, has hydrogel-like properties that keep cell surfaces well hydrated, segregated from neighboring cells, and resistant to non-specific protein deposition. With the molecular details of cell surface carbohydrates now in hand, adaptation of these structural motifs to synthetic materials is an appealing strategy for improving biocompatibility. The goal of this collaborative project between Prof. Bertozzi's research group, the Center for Advanced Materials at Lawrence Berkeley National Laboratory and Sunsoft Corporation was the design, synthesis and characterization of novel hydrogel polymers for improved soft contact lens materials. Our efforts were motivated by the urgent need for improved materials that allow extended wear, and essential feature for those whose occupation requires the use of contact lenses rather than traditional spectacles. Our strategy was to transplant the chemical features of cell surface molecules into contact lens materials so that they more closely resemble the tissue in which they reside. Specifically, we integrated carbohydrate molecules similar to those found on cell surfaces, and sulfoxide materials inspired by the properties of the carbohydrates, into hydrogels composed of biocompatible and manufacturable substrates. The new materials were characterized with respect to surface and bulk hydrophilicity

  10. Biocompatible multi-walled carbon nanotube–CdTe quantum dot–polymer hybrids for medical applications

    Energy Technology Data Exchange (ETDEWEB)

    Baslak, Canan, E-mail: cananbaslak@gmail.com [Advanced Technology Research and Application Center, Selcuk University, 42075 Konya (Turkey); Department of Chemistry, Faculty of Science, Selcuk University, 42075 Konya (Turkey); Demirel Kars, Meltem, E-mail: dmeltem@yahoo.com [Advanced Technology Research and Application Center, Selcuk University, 42075 Konya (Turkey); Sarayonu Vocational High School, Selcuk University, 42430 Konya (Turkey); Karaman, Mustafa; Kus, Mahmut [Advanced Technology Research and Application Center, Selcuk University, 42075 Konya (Turkey); Department of Chemical Engineering, Faculty of Engineering, Selcuk University, 42075 Konya (Turkey); Cengeloglu, Yunus; Ersoz, Mustafa [Advanced Technology Research and Application Center, Selcuk University, 42075 Konya (Turkey); Department of Chemistry, Faculty of Science, Selcuk University, 42075 Konya (Turkey)

    2015-04-15

    Herein we report the synthesis of polymer coated quantum dots (QDs)–carbon nanotube composite material with high biocompatibility and low cellular toxicity. The synthesized multi-walled carbon nanotube (MWCNT)–QD-(-poly(glycidyl methacrylate)) (pGMA) hybrids were characterized using X-ray photoelectron spectroscopy, laser scanning confocal microscopy, transmission electron microscopy and scanning electron microscopy. The results showed that quantum dots were well-distributed on nanotube surfaces in high density. The toxicological assessments of QDs and MWCNT–QD–polymer hybrids in human mammary carcinoma cells and their fluorescence imaging in living cell system were carried out. MWCNT–QD–polymer hybrids possess intense red fluorescence signal under confocal microscopy and good fluorescence stability over 6-h exposure in living cell system. The toxicity comparison of QDs and MWCNT–QD–polymer hybrids has shown that the existence of PGMA thin coating on MWCNT–QD hybrid surface decreased the cellular toxicity and increased biocompatibility. - Highlights: • We report that polymer coating of QDs on CNTs increased their biocompatibility by decreasing cellular toxicity. • QD–CNT polymer hybrid material may be proposed as a good diagnostic agent to visualize cancer cells which may be improved as a therapeutic carrier in future. • Coating QDs with polymer seems to be a right choice to be used in medicinal applications both for diagnosis and for therapy.

  11. Biocompatible multi-walled carbon nanotube–CdTe quantum dot–polymer hybrids for medical applications

    International Nuclear Information System (INIS)

    Herein we report the synthesis of polymer coated quantum dots (QDs)–carbon nanotube composite material with high biocompatibility and low cellular toxicity. The synthesized multi-walled carbon nanotube (MWCNT)–QD-(-poly(glycidyl methacrylate)) (pGMA) hybrids were characterized using X-ray photoelectron spectroscopy, laser scanning confocal microscopy, transmission electron microscopy and scanning electron microscopy. The results showed that quantum dots were well-distributed on nanotube surfaces in high density. The toxicological assessments of QDs and MWCNT–QD–polymer hybrids in human mammary carcinoma cells and their fluorescence imaging in living cell system were carried out. MWCNT–QD–polymer hybrids possess intense red fluorescence signal under confocal microscopy and good fluorescence stability over 6-h exposure in living cell system. The toxicity comparison of QDs and MWCNT–QD–polymer hybrids has shown that the existence of PGMA thin coating on MWCNT–QD hybrid surface decreased the cellular toxicity and increased biocompatibility. - Highlights: • We report that polymer coating of QDs on CNTs increased their biocompatibility by decreasing cellular toxicity. • QD–CNT polymer hybrid material may be proposed as a good diagnostic agent to visualize cancer cells which may be improved as a therapeutic carrier in future. • Coating QDs with polymer seems to be a right choice to be used in medicinal applications both for diagnosis and for therapy

  12. Synthesis and characterization of polyhedral oligomeric titanized silsesquioxane: A new biocompatible cage like molecule for biomedical application.

    Science.gov (United States)

    Yahyaei, Hossein; Mohseni, Mohsen; Ghanbari, Hossein; Messori, Massimo

    2016-04-01

    Organic-inorganic hybrid materials have shown improved properties to be used as biocompatible coating in biomedical applications. Polyhedral oligomeric silsesquioxane (POSS) containing coatings are among hybrid materials showing promising properties for these applications. In this work an open cage POSS has been reacted with a titanium alkoxide to end cap the POSS molecule with titanium atom to obtain a so called polyhedral oligomeric metalized silsesquioxane (POMS). The synthesized POMS was characterized by FTIR, RAMAN and UV-visible spectroscopy as well as (29)Si NMR and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) techniques. Appearance of peaks at 920cm(-1) in FTIR and 491cm(-1) and 1083cm(-1) in Raman spectra confirmed Si-O-Ti linkage formation. It was also demonstrated that POMS was in a monomeric form. To evaluate the biocompatibility of hybrids films, pristine POSS and synthesized POMS were used in synthesis of a polycarbonate urethane polymer. Results revealed that POMS containing hybrid, not only had notable thermal and mechanical stability compared to POSS containing one, as demonstrated by DSC and DMTA analysis, they also showed controlled surface properties in such a manner that hydrophobicity and biocompatibility were both reachable to give rise to improved cell viability in presence of human umbilical vein endothelial cells (HUVEC) and MRC-5 cells. PMID:26838853

  13. Reengineered graft copolymers as a potential alternative for the bone tissue engineering application by inducing osteogenic markers expression and biocompatibility.

    Science.gov (United States)

    Thangavelu, Muthukumar; R Narasimha, Raghavan; Adithan, Aravinthan; A, Chandrasekaran; Jong-Hoon, Kim; Thotapalli Parvathaleswara, Sastry

    2016-07-01

    Composite scaffolds of nano-hydroxyapatite with demineralized bone matrix were prepared and they were graft copolymerized for better bone regeneration and drug delivery applications. The graft copolymers were characterized for their physiochemical properties using conventional methods like FTIR, TGA, XRD and SEM. The scaffolds were seeded with 3T3 and MG63 cells for studying their biocompatibility and their temporal expression of ALP activity, the rate of calcium deposition and their gene expression of collagen type I (Coll-1), osteopontin (OP), osteonectin (ON), and osteocalcin (OC) were studied. In vivo studies were conducted using sub-cutaneous implantation models in male Wister rats for 6 months. Periodic radiography and post-autopsy histopathology was analysed at 15days, 1, 2, 3, 4, 5, and 6 months. The obtained in vitro results clearly confirm that the bone scaffolds prepared in this study are biocompatible, superior osteoinductivity, capable of supporting growth, maturation of MG 63 osteoblast like cells; the gene expression profile revealed that the material is capable of supporting the in vitro growth and maturation of osteoblast-like cells and maturation. The in vivo results stand a testimony to the in vitro results in proving the biocompatibility and osteoinductivity of the materials. PMID:26998863

  14. Effect of coupling asynchronous acoustoelectric effects on the corrosion behavior, microhardness and biocompatibility of biomedical titanium alloy strips.

    Science.gov (United States)

    Ye, Xiaoxin; Tang, Guoyi

    2015-01-01

    The coupling asynchronous acoustoelectric effects (CAAE) of the high-energy electropulsing treatment (EPT) technique and ultrasonic surface strengthening modification (USSM) are innovatively combined in improving the surface microhardness, corrosion behavior and biocompatibility of the pre-deformed titanium alloy strips. Experimental results show that EPT and USSM processes facilitate the surface grain refining and USSM brings in the micro-dimples on the materials surface, which is attributed to the atoms diffusion acceleration under EPT and severe surface plastic deformation under USSM. These microstructure changes can not only enhance the corrosion resistance in the acidic simulated body fluids and fluoridated acidic artificial saliva but also improve the biocompatibility of the titanium alloy strip materials. Moreover, the surface microhardness of the titanium alloy strips is enhanced to improve the wear resistance. Therefore, CAAE processing is a high-efficiency and energy-saving method for obtaining biomedical titanium alloys with superior anti-corrosion performance, microhardness and biocompatibility, which can be widely applied in dental implants and artificial joint. PMID:25596862

  15. BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL

    Directory of Open Access Journals (Sweden)

    NURSHUHADA MOHD NAZIR

    2012-04-01

    Full Text Available β-Tricalcium Phosphate (β–TCP have been widely used as an implant materials. It has been successfully produced locally using two different method which is hydrothermal and precipitation. The aim of the study was to determine the biocompatibility of β-TCP prepared by hydrothermal and precipitation method with normal human osteoblast (NHOst cells. For this purpose cytotoxicity of the material was assessed using an Alamar Blue method to determine the viability of NHOst cells grown with extracts of β-TCP in various concentrations. In addition NHOst grown on β-TCP ceramics were examined under an inverted microscope after 4, 24, 48 and 72 hours to verify cell attachment. Staining was done using Calcein AM and Ethidium homodimers to assess viability using a Confocal Laser Scanning Microscope (CLSM. The results showed that neither hydrothermal β-TCP nor precipitation β-TCP were cytotoxic with either of the method applied.

  16. Photophysical properties and biocompatibility of Photoluminescent Y2O3:Eu nanoparticles in polymethylmetacrylate matrix.

    Science.gov (United States)

    Abe, Shigeaki; Hamba, Yusuke; Iwadera, Nobuki; Yamagata, Shuichi; Yawaka, Yasutaka; Uo, Motohiro; Iida, Junichiro; Kiba, Takayuki; Murayama, Akihiro; Watari, Fumio

    2014-04-01

    In this study, we produced europium-doped yttoria (Y2O3:Eu) nanoparticles and investigated their photoluminescent properties and biocompatibility. The Y2O3:Eu nanoparticles showed excellent photoluminescent properties and cytocompatibility. We also analyzed the photophysical properties of the nanoparticles in PMMA films. When the Y2O3:Eu nanoparticles were incorporated in the polymer film, they showed a strong red emission spectrum, similar to that seen with the particles alone. Energy dispersive X-ray spectroscopy (EDS) measurements indicated that the particles were distributed homogeneously in the PMMA film. Such materials could be applied not only to optoelectronic devices but also to biomedical applications such as bioimaging tools or luminescent medical/dental adhesive materials. PMID:24734706

  17. Dissolution chemistry and biocompatibility of silicon- and germanium-based semiconductors for transient electronics.

    Science.gov (United States)

    Kang, Seung-Kyun; Park, Gayoung; Kim, Kyungmin; Hwang, Suk-Won; Cheng, Huanyu; Shin, Jiho; Chung, Sangjin; Kim, Minjin; Yin, Lan; Lee, Jeong Chul; Lee, Kyung-Mi; Rogers, John A

    2015-05-01

    Semiconducting materials are central to the development of high-performance electronics that are capable of dissolving completely when immersed in aqueous solutions, groundwater, or biofluids, for applications in temporary biomedical implants, environmentally degradable sensors, and other systems. The results reported here include comprehensive studies of the dissolution by hydrolysis of polycrystalline silicon, amorphous silicon, silicon-germanium, and germanium in aqueous solutions of various pH values and temperatures. In vitro cellular toxicity evaluations demonstrate the biocompatibility of the materials and end products of dissolution, thereby supporting their potential for use in biodegradable electronics. A fully dissolvable thin-film solar cell illustrates the ability to integrate these semiconductors into functional systems. PMID:25867894

  18. Comparison of biocompatibility and adsorption properties of different plastics for advanced microfluidic cell and tissue culture models.

    Science.gov (United States)

    van Midwoud, Paul M; Janse, Arnout; Merema, Marjolijn T; Groothuis, Geny M M; Verpoorte, Elisabeth

    2012-05-01

    Microfluidic technology is providing new routes toward advanced cell and tissue culture models to better understand human biology and disease. Many advanced devices have been made from poly(dimethylsiloxane) (PDMS) to enable experiments, for example, to study drug metabolism by use of precision-cut liver slices, that are not possible with conventional systems. However, PDMS, a silicone rubber material, is very hydrophobic and tends to exhibit significant adsorption and absorption of hydrophobic drugs and their metabolites. Although glass could be used as an alternative, thermoplastics are better from a cost and fabrication perspective. Thermoplastic polymers (plastics) allow easy surface treatment and are generally transparent and biocompatible. This study focuses on the fabrication of biocompatible microfluidic devices with low adsorption properties from the thermoplastics poly(methyl methacrylate) (PMMA), polystyrene (PS), polycarbonate (PC), and cyclic olefin copolymer (COC) as alternatives for PDMS devices. Thermoplastic surfaces were oxidized using UV-generated ozone or oxygen plasma to reduce adsorption of hydrophobic compounds. Surface hydrophilicity was assessed over 4 weeks by measuring the contact angle of water on the surface. The adsorption of 7-ethoxycoumarin, testosterone, and their metabolites was also determined after UV-ozone treatment. Biocompatibility was assessed by culturing human hepatoma (HepG2) cells on treated surfaces. Comparison of the adsorption properties and biocompatibility of devices in different plastics revealed that only UV-ozone-treated PC and COC devices satisfied both criteria. This paper lays an important foundation that will help researchers make informed decisions with respect to the materials they select for microfluidic cell-based culture experiments. PMID:22444457

  19. Simple replica micromolding of biocompatible styrenic elastomers.

    Science.gov (United States)

    Borysiak, Mark D; Bielawski, Kevin S; Sniadecki, Nathan J; Jenkel, Colin F; Vogt, Bryan D; Posner, Jonathan D

    2013-07-21

    In this work, we introduce a simple solvent-assisted micromolding technique for the fabrication of high-fidelity styrene-ethylene/butylene-styrene (SEBS) microfluidic devices with high polystyrene (PS) content (42 wt% PS, SEBS42). SEBS triblock copolymers are styrenic thermoplastic elastomers that exhibit both glassy thermoplastic and elastomeric properties resulting from their respective hard PS and rubbery ethylene/butylene segments. The PS fraction gives SEBS microdevices many of the appealing properties of pure PS devices, while the elastomeric properties simplify fabrication of the devices, similar to PDMS. SEBS42 devices have wettable, stable surfaces (both contact angle and zeta potential) that support cell attachment and proliferation consistent with tissue culture dish substrates, do not adsorb hydrophobic molecules, and have high bond strength to wide range of substrates (glass, PS, SEBS). Furthermore, SEBS42 devices are mechanically robust, thermally stable, as well as exhibit low auto-fluorescence and high transmissivity. We characterize SEBS42 surface properties by contact angle measurements, cell culture studies, zeta potential measurements, and the adsorption of hydrophobic molecules. The PS surface composition of SEBS microdevices cast on different substrates is determined by time-of-flight secondary ion mass spectrometry (ToF-SIMS). The attractive SEBS42 material properties, coupled with the simple fabrication method, make SEBS42 a quality substrate for microfluidic applications where the properties of PS are desired but the ease of PDMS micromolding is favoured. PMID:23670166

  20. Structural and degradation studies of a biocompatible Zn-L-tartrate metal–organic framework

    Energy Technology Data Exchange (ETDEWEB)

    Palǒić, Ana; Puškarić, Andreas [Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb (Croatia); Mazaj, Matjaž, E-mail: matjaz.mazaj@ki.si [National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana (Slovenia); Žunkoviǒ, Emanuela; Logar, Nataša Zabukovec [National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana (Slovenia); Bronić, Josip [Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb (Croatia)

    2015-05-15

    New anhydrous Zn-L-tratrate was synthesized under hydrothermal conditions. Crystal structure which was solved by powder X-ray diffraction data from monoclinic unit cell (I{sub 2}, a=11.7557(1), b=9.0319(1), c=5.0750(1), β=91.920(1)) consists of the three-dimensional framework where each Zn atom coordinates four tartrate ligands in distorted octahedral geometry. Due to its biocompatible components, the degradation of material was studied in aqueous media under different pH values (pH=1–7) and in the simulated body fluid (pH=7.4) performed by the measurement of the released Zn{sup 2+} concentrations at thermostatic conditions (37 °C). The results showed that under acidic conditions approximately 60% of Zn{sup 2+} is released from the investigated material after 6 h of degradation process. With the increase of the solution’s pH, the rate of degradation gradually decreases. XRD and SEM analyses indicate that in aqueous media the compound slowly disintegrates and does not undergo hydrolysis process regardless of pH. Biocompatibility of the compound and its controllable rate of degradation even in acid conditions open its potential use in the fields of bio-applications. - Graphical abstract: The degradation kinetics of Zn-L-tar MOF material in aqueous media at different pH conditions. - Highlights: • Anhydrous chiral Zn-L-tratrate was synthesized under hydrothermal conditions. • 3-D framework consists of Zn atoms, each chelating four tartrate ligands. • Degradation of material was studied in aqueous media at different pH values. • The compound disintegrates in controllable manner in aqueous solutions. • The rate compound degradation is increasing with increased acidity of solution.

  1. Structural and degradation studies of a biocompatible Zn-L-tartrate metal–organic framework

    International Nuclear Information System (INIS)

    New anhydrous Zn-L-tratrate was synthesized under hydrothermal conditions. Crystal structure which was solved by powder X-ray diffraction data from monoclinic unit cell (I2, a=11.7557(1), b=9.0319(1), c=5.0750(1), β=91.920(1)) consists of the three-dimensional framework where each Zn atom coordinates four tartrate ligands in distorted octahedral geometry. Due to its biocompatible components, the degradation of material was studied in aqueous media under different pH values (pH=1–7) and in the simulated body fluid (pH=7.4) performed by the measurement of the released Zn2+ concentrations at thermostatic conditions (37 °C). The results showed that under acidic conditions approximately 60% of Zn2+ is released from the investigated material after 6 h of degradation process. With the increase of the solution’s pH, the rate of degradation gradually decreases. XRD and SEM analyses indicate that in aqueous media the compound slowly disintegrates and does not undergo hydrolysis process regardless of pH. Biocompatibility of the compound and its controllable rate of degradation even in acid conditions open its potential use in the fields of bio-applications. - Graphical abstract: The degradation kinetics of Zn-L-tar MOF material in aqueous media at different pH conditions. - Highlights: • Anhydrous chiral Zn-L-tratrate was synthesized under hydrothermal conditions. • 3-D framework consists of Zn atoms, each chelating four tartrate ligands. • Degradation of material was studied in aqueous media at different pH values. • The compound disintegrates in controllable manner in aqueous solutions. • The rate compound degradation is increasing with increased acidity of solution

  2. Stability and biocompatibility of photothermal gold nanorods after lyophilization and sterilization

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, Leyre [Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), C/ Mariano Esquillor, R and D Building, University of Zaragoza, 50018 Zaragoza (Spain); Cebrian, Virginia [CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza (Spain); Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid (Spain); Martin-Saavedra, Francisco [Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid (Spain); CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza (Spain); Arruebo, Manuel, E-mail: arruebom@unizar.es [Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), C/ Mariano Esquillor, R and D Building, University of Zaragoza, 50018 Zaragoza (Spain); CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza (Spain); Vilaboa, Nuria [Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid (Spain); CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza (Spain); Santamaria, Jesus, E-mail: Jesus.Santamaria@unizar.es [Department of Chemical Engineering, Nanoscience Institute of Aragon (INA), C/ Mariano Esquillor, R and D Building, University of Zaragoza, 50018 Zaragoza (Spain); CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza (Spain)

    2013-10-15

    Graphical abstract: - Highlights: • Morphological changes are observed for CTABr capped gold nanorods over time. • Polystyrenesulfonate (PSS) and polyethyleneglycol (PEG) coated nanorods are stable. • Re-suspendible and sterilizable colloids are prepared using those capping agents. • Those materials are efficient heat sinks potentially used in photothermal therapy. - Abstract: Suspensions in phosphate buffered saline (PBS) of gold nanorods stabilized with cetyltrimethyl ammonium chloride (CTABr), polystyrenesulfonate (PSS) and methyl-polyethyleneglycol-thiol (m-PEG-SH) have been prepared and the evolution of their colloidal stability and plasmonic response over time has been evaluated. Their performance after lyophilization, alcoholic sterilization and resuspension has also been characterized. Sub-cytotoxic doses on HeLa cells were calculated for the three surface functionalizations used. Their heating efficiency at different exposure times was also evaluated after being irradiated with near infrared light. The best results were obtained for m-PEG-SH stabilized rods, which were not only stable, sterilizable and lyophilizable, but also biocompatible at all doses tested, showing potential as a stable, re-suspendible and biocompatible hyperthermic agent.

  3. Biocompatibility and antimicrobial activity of zinc(II doped hydroxyapatite, synthesized by hydrothermal method

    Directory of Open Access Journals (Sweden)

    Kojić Vesna

    2012-01-01

    Full Text Available In order to obtain multifunctional materials with good biocompatibility and antimicrobial effect, hydroxyapatite (HAp doped with Zn2+ was synthesized by hydrothermal method. Powders with different content of zinc ions were synthesized and compared with undoped HAp to investigation of Zn2+ ion influence on the antimicrobial activity of HAp. Analyses of undoped and Zn2+-doped powders before and after thermal treatment at 1200ºC were performed by SEM and XRD. Antimicrobial effects of powders were examined in relation to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans in liquid medium. The results showed that the obtained powders have good antimicrobial activity, but higher antimicrobial activities of powders doped with Zn2+ were observed after annealing at 1200°C. For powders annealed at 1200°C in vitro biocompatibility tests MTT and DET with MRC-5 fibroblast cells in liquid medium were carried out. Based on MTT and DET tests it was shown that powders do not have a significant cytotoxic effect, which was confirmed by SEM analysis of MRC-5 fibroblast cells after theirs in vitro contact with powders. [Projekat Ministarstva nauke Republike Srbije, br. III 45019 and FP7-REGPOT-2009-1 NANOTECH FTM

  4. Physicochemical properties and biocompatibility of chitosan oligosaccharide/gelatin/calcium phosphate hybrid cements

    International Nuclear Information System (INIS)

    A bone substitute material was developed consisting of a chitosan oligosaccharide (COS) solution in a liquid phase and gelatin (GLT) containing calcium phosphate powder in a solid phase. The physicochemical and biocompatible properties of the hybrid cements were evaluated. The addition of COS to cement did not affect the setting time or diametral tensile strength of the hybrid cements, whereas GLT significantly prolonged the setting time and decreased the strength slightly. The setting reaction was inhibited by the addition of GLT to the initial mixture, but not by COS. However, the presence of GLT appreciably improved the anti-washout properties of the hybrid cement compared with COS. COS may promote the cement's biocompatibility as an approximate twofold increase in cell proliferation for 10% COS-containing cements was observed on day 3 as compared with the controls. The combination of GLT and COS was chosen due to the benefits achieved from several synergistic effects and for their clinical applications. Cement with 5% GLT and 10% COS may be a better choice among cements in terms of anti-washout properties and biological activity.

  5. Surface morphology, tribological properties and in vitro biocompatibility of nanostructured zirconia thin films.

    Science.gov (United States)

    Bianchi, M; Gambardella, A; Berni, M; Panseri, S; Montesi, M; Lopomo, N; Tampieri, A; Marcacci, M; Russo, A

    2016-05-01

    Deposition of nanostructured and low-wear zirconia (ZrO2) thin films on the metallic component of a total joint implant is envisaged to reduce wear of the soft ultra-high molecular weight polyethylene (UHMWPE) counterpart. In this work, morphological surface features, wear resistance and in vitro-biocompatibility of zirconia thin films deposited by the novel Pulsed Plasma Deposition (PPD) method have been investigated. Film thickness, roughness and wettability were found to be strongly dependent on deposition gas pressure. Interestingly, wear rate of UHMWPE disks coupled to zirconia-coated titanium spheres was only poorly correlated to the contact angle values, while film roughness and thickness seemed not to affect it. Furthermore, wear of UHMWPE, when coupled with zirconia coated-titanium spheres, significantly decreased with respect to uncoated spheres under dry or NaCl-lubricated conditions; besides, when using bovine serum, similar results were obtained for coated and uncoated spheres. Finally, suitable mesenchymal stem and osteoblast cells adhesion, proliferation and viability were observed, suggesting good biocompatibility of the nanostructured zirconia films. Taken together, the results shown in this work indicate that zirconia thin films deposited by the PPD method deserve further investigations as low-wear materials for biomedical applications such as total joint replacement. PMID:27003838

  6. Understanding the Biocompatibility of Sintered Calcium Phosphate with Ratio of [Ca]/[P] = 1.50

    Directory of Open Access Journals (Sweden)

    Feng-Lin Yen

    2012-01-01

    Full Text Available Biocompatibility of sintered calcium phosphate pellets with [Ca]/[P] = 1.50 was determined in this study. Calcium pyrophosphate (CPP phase formed on the sintered pellets immersed in a normal saline solution for 14 d at 37∘C. The intensities of hydroxyapatite (HA reflections in the X-ray diffraction (XRD patterns of the pellets were retrieved to as-sintered state. The pellet surface morphology shows that CPP crystallites were clearly present and make an amorphous calcium phosphate (ACP to discriminate against become to the area of slice join together. In addition, the intensities of the CPP reflections in the XRD patterns were the highest when the pellets were immersed for 28 d. When the CPP powders were extracted from the pellets after immersion in the solution for 14 d, the viability of 3T3 cells remained above 90% for culture times from 1 to 4 d. The pellet surface morphology observed using optical microscopy showed that the cells did not adhere to the bottom of the sintered pellets when cultured for 4 d; however, some CPP phase precipitates were formed, as confirmed by XRD. In consequence, the results suggest that the sintered HA powders are good materials for use in biomedical applications because of their good biocompatibility.

  7. Mechanical performance of a biocompatible biocide soda-lime glass-ceramic.

    Science.gov (United States)

    López-Esteban, S; Bartolomé, J F; Dí Az, L A; Esteban-Tejeda, L; Prado, C; López-Piriz, R; Torrecillas, R; Moya, J S

    2014-06-01

    A biocompatible soda-lime glass-ceramic in the SiO2-Na2O-Al2O3-CaO-B2O3 system containing combeite and nepheline as crystalline phases, has been obtained at 750°C by two different routes: (i) pressureless sintering and (ii) Spark Plasma Sintering. The SPS glass-ceramic showed a bending strength, Weibull modulus, and toughness similar values to the cortical human bone. This material had a fatigue limit slightly superior to cortical bone and at least two times higher than commercial dental glass-ceramics and dentine. The in vitro studies indicate that soda-lime glass-ceramic is fully biocompatible. The in vivo studies in beagle jaws showed that implanted SPS rods presented no inflammatory changes in soft tissues surrounding implants in any of the 10 different cases after four months implantation. The radiological analysis indicates no signs of osseointegration lack around implants. Moreover, the biocide activity of SPS glass-ceramic versus Escherichia coli, was found to be >4log indicating that it prevents implant infections. Because of this, the SPS new glass-ceramic is particularly promising for dental applications (inlay, crowns, etc). PMID:24667693

  8. Structural and biocompatible characterization of TiC/a:C nanocomposite thin films

    International Nuclear Information System (INIS)

    In this work, sputtered TiC/amorphous C thin films have been developed in order to be applied as potential barrier coating for interfering of Ti ions from pure Ti or Ti alloy implants. Our experiments were based on magnetron sputtering method, because the vacuum deposition provides great flexibility for manipulating material chemistry and structure, leading to films and coatings with special properties. The films have been deposited on silicon (001) substrates with 300 nm thick oxidized silicon sublayer at 200 °C deposition temperature as model substrate. Transmission electron microscopy has been used for structural investigations. Thin films consisted of ∼ 20 nm TiC columnar crystals embedded by 5 nm thin amorphous carbon matrix. MG63 osteoblast cells have been applied for in vitro study of TiC nanocomposites. The cell culture tests give strong evidence of thin films biocompatibility. Highlights: ► The main goal of this work is the relatively easy preparation of nanocomposite TiC thin films by dc magnetron sputtering. ► TEM and HREM were applied for structural characterization of columnar TiC nanocrystals and amorphous carbon matrix. ► The biocompatibility of films was showed by MG63 human osteoblast like cells during 1, 3 and 7 days seeding

  9. Stability and biocompatibility of photothermal gold nanorods after lyophilization and sterilization

    International Nuclear Information System (INIS)

    Graphical abstract: - Highlights: • Morphological changes are observed for CTABr capped gold nanorods over time. • Polystyrenesulfonate (PSS) and polyethyleneglycol (PEG) coated nanorods are stable. • Re-suspendible and sterilizable colloids are prepared using those capping agents. • Those materials are efficient heat sinks potentially used in photothermal therapy. - Abstract: Suspensions in phosphate buffered saline (PBS) of gold nanorods stabilized with cetyltrimethyl ammonium chloride (CTABr), polystyrenesulfonate (PSS) and methyl-polyethyleneglycol-thiol (m-PEG-SH) have been prepared and the evolution of their colloidal stability and plasmonic response over time has been evaluated. Their performance after lyophilization, alcoholic sterilization and resuspension has also been characterized. Sub-cytotoxic doses on HeLa cells were calculated for the three surface functionalizations used. Their heating efficiency at different exposure times was also evaluated after being irradiated with near infrared light. The best results were obtained for m-PEG-SH stabilized rods, which were not only stable, sterilizable and lyophilizable, but also biocompatible at all doses tested, showing potential as a stable, re-suspendible and biocompatible hyperthermic agent

  10. Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells.

    Science.gov (United States)

    Choi, Kyong-Hoon; Nam, Ki Chang; Malkinski, Leszek; Choi, Eun Ha; Jung, Jin-Seung; Park, Bong Joo

    2016-01-01

    In this study, newly designed biocompatible multifunctional magnetic submicron particles (CoFe₂O₄-HPs-FAs) of well-defined sizes (60, 133, 245, and 335 nm) were fabricated for application as a photosensitizer delivery agent for photodynamic therapy in cancer cells. To provide selective targeting of cancer cells and destruction of cancer cell functionality, basic cobalt ferrite (CoFe₂O₄) particles were covalently bonded with a photosensitizer (PS), which comprises hematoporphyrin (HP), and folic acid (FA) molecules. The magnetic properties of the CoFe₂O₄ particles were finely adjusted by controlling the size of the primary CoFe₂O₄ nanograins, and secondary superstructured composite particles were formed by aggregation of the nanograins. The prepared CoFe₂O₄-HP-FA exhibited high water solubility, good MR-imaging capacity, and biocompatibility without any in vitro cytotoxicity. In particular, our CoFe₂O₄-HP-FA exhibited remarkable photodynamic anticancer efficiency via induction of apoptotic death in PC-3 prostate cancer cells in a particle size- and concentration-dependent manner. This size-dependent effect was determined by the specific surface area of the particles because the number of HP molecules increased with decreasing size and increasing surface area. These results indicate that our CoFe₂O₄-HP-FA may be applicable for photodynamic therapy (PDT) as a PS delivery material and a therapeutic agent for MR-imaging based PDT owing to their high saturation value for magnetization and superparamagnetism. PMID:27607999

  11. Biocompatibility assessment of fibrous nanomaterials in mammalian embryos.

    Science.gov (United States)

    Munk, Michele; Camargo, Luiz S A; Quintão, Carolina C R; Silva, Saulo R; Souza, Eliza D; Raposo, Nádia R B; Marconcini, Jose M; Jorio, Ado; Ladeira, Luiz O; Brandão, Humberto M

    2016-07-01

    Currently there is a growing interest in the use of nanotechnology in reproductive medicine and reproductive biology. However, their toxic effects on mammalian embryos remain poorly understood. In this work, we evaluate the biocompatibility of two fibrous nanomaterials (NMs): cotton cellulose nanofibers (CNF) and carboxylated multiwalled carbon nanotubes (MWCNT-COOH), by performing an investigation of the embryonic development, gene expression (biomarkers focused on cell stress, apoptosis and totipotency) and in situ apoptosis in bovine embryos. Exposure to NMs did not interfere in preimplantation development or in the incidence of apoptosis in the bovine embryo, but they did affect the gene expression. The results presented are important for an understanding of the toxicity of cotton CNF and MWCNT-COOH on mammalian embryos. To our knowledge, we report the first evaluation of biocompatibility between these NMs on preimplantation embryos, which may open a new window for reproductive biomedical applications. PMID:26949162

  12. Interfacing Microbial Styrene Production with a Biocompatible Cyclopropanation Reaction**

    OpenAIRE

    Wallace, Stephen; Balskus, Emily P.

    2015-01-01

    Introducing new reactivity into living organisms is a major challenge in synthetic biology. Despite an increasing interest in both developing aqueous-compatible small molecule catalysts and engineering enzymes to perform new chemistry in vitro, the integration of non-native reactivity into metabolic pathways for small molecule production has been underexplored. Herein we report a biocompatible iron(III) phthalocyanine catalyst capable of efficient olefin cyclopropanation in the presence of a ...

  13. Biomolecular modification of zirconia surfaces for enhanced biocompatibility

    International Nuclear Information System (INIS)

    Yttria-tetragonal zirconia polycrystal (Y-TZP) is a preferred biomaterial due to its good mechanical properties. In order to improve the biocompatibility of zirconia, RGD-peptide derived from extracellular matrix proteins was employed to modify the surface of Y-TZP to promote cell adhesion in this study. The surface of Y-TZP specimens was first modified using a hydrothermal method for different lengths of time. The topographies of modified Y-TZP specimens were analyzed by contact angle, XRD, FTIR, AFM, and FE-SEM. The mechanical properties were evaluated using Vickers hardness and three point bending strength. Then, the RGD-peptide was immobilized on the surface of the Y-TZP by chemical treatment. These RGD-peptide immobilized Y-TZP specimens were characterized by FTIR and AFM, and then were cocultured with MG-63 osteoblast cells for biocompatibility assay. The cell morphology and proliferation were evaluated by SEM, WST-1, and ALP activity assay. The XRD results indicated that the phase transition, from tetragonal phase to monoclinic phase, was increased with a longer incubation time of hydrothermal treatment. However, there were no significant differences in mechanical strengths after RGD-peptide was successfully grafted onto the Y-TZP surface. The SEM images showed that the MG-63 cells appeared polygonal, spindle-shaped, and attached on the RGD-peptide immobilized Y-TZP. The proliferation and cellular activities of MG-63 cells on the RGD-peptide immobilized Y-TZP were better than that on the unmodified Y-TZP. From the above results, the RGD-peptide can be successfully grafted onto the hydrothermal modified Y-TZP surface. The RGD-peptide immobilized Y-TZP can increase cell adhesion, and thus, improve the biocompatibility of Y-TZP. - Highlights: • Covalent bonding between peptide and Y-TZP was proposed. • Stable biomimetic structures produced on the surface of zirconia. • The biocompatibility was improved

  14. Biocompatible polymer microarrays for cellular high-content screening

    OpenAIRE

    Pernagallo, Salvatore

    2010-01-01

    The global aim of this thesis was to study the use of microarray technology for the screening and identification of biocompatible polymers, to understand physiological phenomena, and the design of biomaterials, implant surfaces and tissue-engineering scaffolds. This work was based upon the polymer microarray platform developed by the Bradley group. Polymer microarrays were successfully applied to find the best polymer supports for: (i) mouse fibroblast cells and used to eval...

  15. Sputtered Gum metal thin films showing bacterial inactivation and biocompatibility.

    Science.gov (United States)

    Achache, S; Alhussein, A; Lamri, S; François, M; Sanchette, F; Pulgarin, C; Kiwi, J; Rtimi, S

    2016-10-01

    Super-elastic Titanium based thin films Ti-23Nb-0.7Ta-2Zr-(O) (TNTZ-O) and Ti-24Nb-(N) (TN-N) (at.%) were deposited by direct current magnetron sputtering (DCMS) in different reactive atmospheres. The effects of oxygen doping (TNTZ-O) and/or nitrogen doping (TN-N) on the microstructure, mechanical properties and biocompatibility of the as-deposited coatings were investigated. Nano-indentation measurements show that, in both cases, 1sccm of reactive gas in the mixture is necessary to reach acceptable values of hardness and Young's modulus. Mechanical properties are considered in relation to the films compactness, the compressive stress and the changes in the grain size. Data on Bacterial inactivation and biocompatibility are reported in this study. The biocompatibility tests showed that O-containing samples led to higher cells proliferation. Bacterial inactivation was concomitant with the observed pH and surface potential changes under light and in the dark. The increased cell fluidity leading to bacterial lysis was followed during the bacterial inactivation time. The increasing cell wall fluidity was attributed to the damage of the bacterial outer cell which losing its capacity to regulate the ions exchange in and out of the bacteria. PMID:27434155

  16. In Vitro Biocompatibility of Endodontic Sealers Incorporating Antibacterial Nanoparticles

    Directory of Open Access Journals (Sweden)

    Itzhak Abramovitz

    2012-01-01

    Full Text Available The main cause of endodontic disease is bacteria. Disinfection is presently achieved by cleaning the root canal system prior to obturation. Following setting, root canal filling is devoid of any antibacterial effect. Endodontic sealers with antimicrobial properties yet biocompatible may enhance root canal therapy. For this purpose, quaternized polyethylenimine nanoparticles which are antibacterial polymers, biocompatible, nonvolatile, and stable may be used. The aim of the present study was to examine the impact of added QPEI on the cytotoxicity of AH Plus, Epiphany, and GuttaFlow endodontic sealers. The effect of these sealers on the proliferation of RAW 264.7 macrophage and L-929 fibroblast cell lines and on the production of TNFα from macrophages was examined. Cell vitality was evaluated using a colorimetric XTT assay. The presence of cytokines was determined by two-site ELISA. Results show that QPEI at 1% concentration does not impair the basic properties of the examined sealers in both macrophages and fibroblast cell lines. Incorporation of 1% QPEI into the sealers did not impair their biocompatibility. QPEI is a potential clinical candidate to improve antibacterial activity of sealers without increasing cytotoxicity.

  17. A green chemistry approach for synthesizing biocompatible gold nanoparticles

    Science.gov (United States)

    Gurunathan, Sangiliyandi; Han, JaeWoong; Park, Jung Hyun; Kim, Jin-Hoi

    2014-05-01

    Gold nanoparticles (AuNPs) are a fascinating class of nanomaterial that can be used for a wide range of biomedical applications, including bio-imaging, lateral flow assays, environmental detection and purification, data storage, drug delivery, biomarkers, catalysis, chemical sensors, and DNA detection. Biological synthesis of nanoparticles appears to be simple, cost-effective, non-toxic, and easy to use for controlling size, shape, and stability, which is unlike the chemically synthesized nanoparticles. The aim of this study was to synthesize homogeneous AuNPs using pharmaceutically important Ganoderma spp . We developed a simple, non-toxic, and green method for water-soluble AuNP synthesis by treating gold (III) chloride trihydrate (HAuCl4) with a hot aqueous extract of the Ganoderma spp . mycelia. The formation of biologically synthesized AuNPs (bio-AuNPs) was characterized by ultraviolet (UV)-visible absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Furthermore, the biocompatibility of as-prepared AuNPs was evaluated using a series of assays, such as cell viability, lactate dehydrogenase leakage, and reactive oxygen species generation (ROS) in human breast cancer cells (MDA-MB-231). The color change of the solution from yellow to reddish pink and strong surface plasmon resonance were observed at 520 nm using UV-visible spectroscopy, and that indicated the formation of AuNPs. DLS analysis revealed the size distribution of AuNPs in liquid solution, and the average size of AuNPs was 20 nm. The size and morphology of AuNPs were investigated using TEM. The biocompatibility effect of as-prepared AuNPs was investigated in MDA-MB-231 breast cancer cells by using various concentrations of AuNPs (10 to 100 μM) for 24 h. Our findings suggest that AuNPs are non-cytotoxic and biocompatible. To the best of our knowledge

  18. Surface construction and biocompatibility of polymeric used for cardiovascular medical device○

    Institute of Scientific and Technical Information of China (English)

    Chen Bao-lin; Wang Dong-an

    2013-01-01

    BACKGROUND:Biomaterials for the treatment of cardiovascular diseases must have anti-thrombotic, anti-biodegradability and anti-infective ability in the blood-contact condition. OBJECTIVE:To investigate the biocompatibility, blood compatibility and cytocompatibility of new implantable (interfered) polymer material (surface) used in cardiovascular tissue engineering. METHODS:The PubMed database and Wanfang database were retrieved for the related articles from 1967 to 2012 with the key words of“biocompatibility, lood compatibility, biomedical materials, biomedical polymer materials”. RESUTLS AND CONCLUSION:The graft copolymer surface and a block copolymer surfactant that can be used as biological materials were in-depth analyzed though analyzing the design requirements of type, application, cardiovascular medical devices and implantable soft tissue substitutes of polymeric biomaterials used for cardiovascular medicine. The results showed that the difference between the surface and noumenon wil be reflected on many molecular layers that extended from the surface to the noumenon, while the two main factors of surface energy and molecular motion determined the noumenon/surface behavior including the noumenon/surface difference and surface phase separation. If the understanding of the noumenon/surface consisting difference should be taking into consideration, the other determinant should be added too, that was the crystal ization behavior of each competent. If the one of the components contained in the graft copolymer surface and a block copolymer surfactant had higher crystal inity, the other components wil be squeezed out;crystal ization could also hindered the motion and diffusion of molecules, and eventual y, the incompatibility degree of two components of copolymers wil affect the phase separation tendency in the noumenon and surface layers.

  19. [Elastomeric impression materials].

    Science.gov (United States)

    Anagnostopoulos, T; Tsokas, K

    1990-01-01

    A review of the literature on elastomeric impression materials, is presented in this paper. The article mentions the composition and the most important properties of the elastomeric impression materials used in dental practice. The clinical significance of these materials, physical and mechanical properties are also emphasized. In addition some new elastomeric impression materials with improved properties and a new (experimental) light-cured impression material, are mentioned. Another part of this article is the biocompatibility of these materials. In the end the great significance of handling is outlined. PMID:2130039

  20. Mechanical properties, in vitro corrosion and biocompatibility of newly developed biodegradable Mg-Zr-Sr-Ho alloys for biomedical applications.

    Science.gov (United States)

    Ding, Yunfei; Lin, Jixing; Wen, Cuie; Zhang, Dongmei; Li, Yuncang

    2016-01-01

    Our previous studies have demonstrated that Mg-Zr-Sr alloys can be anticipated as excellent biodegradable implant materials for load-bearing applications. In general, rare earth elements (REEs) are widely used in magnesium (Mg) alloys with the aim of enhancing the mechanical properties of Mg-based alloys. In this study, the REE holmium (Ho) was added to an Mg-1Zr-2Sr alloy at different concentrations of Mg1Zr2SrxHo alloys (x = 0, 1, 3, 5 wt. %) and the microstructure, mechanical properties, degradation behaviour and biocompatibility of the alloys were systematically investigated. The results indicate that the addition of Ho to Mg1Zr2Sr led to the formation of the intermetallic phases MgHo3, Mg2Ho and Mg17Sr2 which resulted in enhanced mechanical strength and decreased degradation rates of the Mg-Zr-Sr-Ho alloys. Furthermore, Ho addition (≤5 wt. %) to Mg-Zr-Sr alloys led to enhancement of cell adhesion and proliferation of osteoblast cells on the Mg-Zr-Sr-Ho alloys. The in vitro biodegradation and the biocompatibility of the Mg-Zr-Sr-Ho alloys were both influenced by the Ho concentration in the Mg alloys; Mg1Zr2Sr3Ho exhibited lower degradation rates than Mg1Zr2Sr and displayed the best biocompatibility compared with the other alloys. PMID:27553403

  1. Effect of carbon nanotube and aluminum oxide addition on plasma-sprayed hydroxyapatite coating's mechanical properties and biocompatibility

    International Nuclear Information System (INIS)

    This study reports on the synthesis of novel bioceramic composite coating of hydroxyapatite (HA) reinforced with carbon nanotubes (CNTs) and aluminum oxide (Al2O3) using plasma spray technique. Fracture toughness of HA-20 wt.% Al2O3 improved by 158% as compared to HA coating whereas HA-18.4 wt.% Al2O3-1.6 wt.% CNT showed an improvement of 300%. Carbon nanotubes provided reinforcement via rebar mechanism. Human fiber osteoblast cell-growth studies showed that biocompatibility of the coating remained unaltered, as Al2O3 retained its bio-inertness and CNT, its bioactivity, within the composite coatings. Composite coating showed lower attachment, but higher proliferation rate, for the osteoblast cells, which has been attributed to the surface roughness. An optimized relation between coating composition, its biocompatibility and mechanical properties was established to predict the most suited coating material for orthopedic implants. HA-Al2O3-CNT composite coating displayed most improved mechanical properties while retaining its biocompatibility.

  2. Antimicrobial activity and biocompatibility of Ag+- and Cu2+-doped biphasic hydroxyapatite/α-tricalcium phosphate obtained from hydrothermally synthesized Ag+- and Cu2+-doped hydroxyapatite

    International Nuclear Information System (INIS)

    Hydroxyapatite (HAp) powders doped with Ag+ or Cu2+ were synthesized by a hydrothermal method in order to obtain biomaterial with an antimicrobial effect. The synthesis was performed with two contents of dopant (Ag+ or Cu2+) by considering both the antimicrobial activities and biocompatibility of the powders. The doped HAp was annealed at 1200 °C for 2 h with the intention of investigating the influence of doping with Ag+ and Cu2+ on the creation of the biphasic HAp/α-tricalcium phosphate (HAp/α-TCP) and determining the antimicrobial activity and biocompatibility of the obtained biphasic powders. Analyses of all powders, undoped and doped HAp and HAp/α-TCP, were performed by Field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and energy-dispersive X-ray spectroscopy (EDS). The in vitro antibacterial activities of the powders were evaluated against: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. All powders showed good antimicrobial activity but generally the powders of doped HAp/α-TCP had more uniform results against all pathogenic microorganisms than the powders of doped HAp. In vitro biocompatibility tests, MTT and DET, were used to evaluate the biocompatibility of Ag+- and Cu2+-doped HAp/α-TCP with MRC-5 human fibroblast cells. These tests confirmed that powders do not have a cytotoxic effect. The HAp/α-TCP powders doped with the lower content of Ag+ and Cu2+ showed especially good biocompatibility. Antimicrobial and biocompatibility tests recommend the Ag+- and Cu2+-doped HAp/α-TCP as promising material for use in reconstructive surgery of bone.

  3. In vitro and in vivo biocompatibility of Ti-6Al-4V titanium alloy and UHMWPE polymer for total hip replacement

    OpenAIRE

    Ngoc Bich Vu; Nhung Hai Truong; Long Thanh Dang; Lan Thi Phi; Nga Thi-Thu Ho; Tuan Ngoc Pham; Trinh Phuong Phan; Phuc Van Pham

    2016-01-01

    Introductions: Joint replacements have considerably improved the quality of life of patients with damaged joints. Over the past 30 years, there has been much effort and investigations in ways to repair damages in joints, including knee and hip joints. Materials for joint production have also been developed. Many improvements have been made in the joint replacement materials to increase their biocompatibility and longevity. This study is aimed at evaluating the in vitro and in vivo biocompatib...

  4. Biocompatible slippery fluid-infused films composed of chitosan and alginate via layer-by-layer self-assembly and their antithrombogenicity.

    Science.gov (United States)

    Manabe, Kengo; Kyung, Kyu-Hong; Shiratori, Seimei

    2015-03-01

    Antifouling super-repellent surfaces inspired by Nepenthes, the pitcher plant, were designed and named slippery liquid-infused porous surfaces (SLIPS). These surfaces repel various simple and complex liquids including water and blood by maintaining a low sliding angle. Previous studies have reported the development of fluorinated SLIPS that are not biocompatible. Here, we fabricated fluid-infused films composed of biodegradable materials and a biocompatible lubricant liquid. The film was constructed using a combination of electrostatic interactions between chitosan and alginate and hydrogen-bonding between alginate and polyvinylpyrrolidone (PVPON) via the layer-by-layer self-assembly method. After chitosan and alginate were cross-linked, the PVPON was removed by increasing the pH to generate porosity from the deconstruction of the hydrogen-bonding. The porous underlayer was hydrophobized and covered by biocompatible almond oil. Blood easily flowed over this biodegradable and biocompatible SLIPS without leaving stains on the surface, and the material is environmentally durable, has a high transmittance of about 90%, and is antithrombogenic. The results of this study suggest that this SLIPS may facilitate the creation of nonfouling medical devices through a low-cost, eco-friendly, and simple process. PMID:25646977

  5. BIOCOMPATIBILITY OF MEDICAL DEVICES BASED ON METALS, CAUSES FORMATION OF PATHOLOGICAL REACTIVITY (A REVIEW OF FOREIGN LITERATURE

    Directory of Open Access Journals (Sweden)

    O. M. Rozhnova

    2015-01-01

    Full Text Available The objective of the research is a review of approaches to the evaluation of biocompatibility of medical devices on the basis of metals and alloys, and to find ways of overcoming the low engraftment of implanted structures. Implantation by artificial materials allows us to regain the use of human organs and tissues and to date has no rivals. The advantage of using metals and alloys for implanted structures is their high reliability in operation, long servicelife, and high functionality. The nature of the interaction between the human body and the implant has an impact on resource use and the durability of the structures. Manufacturers of scientific research into medical implants at the present stage are directed to obtain materials that will not adversely affect the human body, and to ensure the maximum survival rate when using them. At the same time, the data presented in the article suggests that attempts to make higher biocompatible material properties tend to reduce the development of new methods for the surface treatment and the chemical composition modulation implants. World literature demonstrates the lack of a systematic approach to the problem of increased sensitivity of patients to different metals and alloys (metal sensitization, resulting in the development of complications such as the development of aseptic inflammation and infectious complications of unstable structures, and loss of functionality. Consequently, there is a need to search for ways to improve the biocompatibility of materials used in medicine, based on an assessment of immune defense mechanisms, and the development of algorithms preoperative tactics. 

  6. Biocompatible Polyhydroxyethylaspartamide-based Micelles with Gadolinium for MRI Contrast Agents

    Directory of Open Access Journals (Sweden)

    Kim Hyo Jeong

    2010-01-01

    Full Text Available Abstract Biocompatible poly-[N-(2-hydroxyethyl-d,l-aspartamide]-methoxypoly(ethyleneglycol-hexadecylamine (PHEA-mPEG-C16 conjugated with 1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid-gadolinium (DOTA-Gd via ethylenediamine (ED was synthesized as a magnetic resonance imaging (MRI contrast agent. Amphiphilic PHEA-mPEG-C16-ED-DOTA-Gd forms micelle in aqueous solution. All the synthesized materials were characterized by proton nuclear magnetic resonance (1H NMR. Micelle size and shape were examined by dynamic light scattering (DLS and atomic force microscopy (AFM. Micelles with PHEA-mPEG-C16-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent. Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C16-ED-DOTA-Gd.

  7. Biocompatible poly(vinylidene fluoride)/cyanoacrylate composite coatings with tunable hydrophobicity and bonding strength

    Science.gov (United States)

    Bayer, I. S.; Tiwari, M. K.; Megaridis, C. M.

    2008-10-01

    Biocompatible composite coatings are produced from solution processable poly(vinylidene fluoride)/cyanoacrylate blends prepared in the presence of rosin and ZnO particle fillers, the latter for control of coating surface microstructure. Dispersions are spray coated and cured at 100 °C onto aluminum foils and fiberglass cloths suitable for tissue engineering applications. The elastic modulus of the composite films matches or exceeds that of polyethylene-based orthopedic implant materials. Contact angle measurements on coated fiberglass cloths reveal that wettability of hydrophobic coatings is maintained under strain for applied mechanical stress levels up to ˜15 kN/m2, whereas ultrahydrophobic coatings fail at ˜5 kN/m2.

  8. Nanocrystalline diamond: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures.

    Science.gov (United States)

    Amaral, M; Dias, A G; Gomes, P S; Lopes, M A; Silva, R F; Santos, J D; Fernandes, M H

    2008-10-01

    Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell culture assays using MG63 osteoblast-like and human bone marrow cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) substrates for NCD coatings based on a previous demonstration of the superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. PMID:18085649

  9. Biocompatible Polyhydroxyethylaspartamide-based Micelles with Gadolinium for MRI Contrast Agents

    Science.gov (United States)

    Jeong, Sang Young; Kim, Hyo Jeong; Kwak, Byung-Kook; Lee, Ha-Young; Seong, Hasoo; Shin, Byung Cheol; Yuk, Soon Hong; Hwang, Sung-Joo; Cho, Sun Hang

    2010-12-01

    Biocompatible poly-[ N-(2-hydroxyethyl)- d, l-aspartamide]-methoxypoly(ethyleneglycol)-hexadecylamine (PHEA-mPEG-C16) conjugated with 1,4,7,10-tetraazacyclododecan-1,4,7,10-tetraacetic acid-gadolinium (DOTA-Gd) via ethylenediamine (ED) was synthesized as a magnetic resonance imaging (MRI) contrast agent. Amphiphilic PHEA-mPEG-C16-ED-DOTA-Gd forms micelle in aqueous solution. All the synthesized materials were characterized by proton nuclear magnetic resonance (1H NMR). Micelle size and shape were examined by dynamic light scattering (DLS) and atomic force microscopy (AFM). Micelles with PHEA-mPEG-C16-ED-DOTA-Gd showed higher relaxivities than the commercially available gadolinium contrast agent. Moreover, the signal intensity of a rabbit liver was effectively increased after intravenous injection of PHEA-mPEG-C16-ED-DOTA-Gd.

  10. BIOCOMPATIBILITY OF MEDICAL DEVICES – LEGAL REGULATIONS IN THE EUROPEAN UNION.

    Directory of Open Access Journals (Sweden)

    Mariela Yaneva-Deliverska

    2015-02-01

    Full Text Available A multiplicity of laws, standards, and recommendations regulate the marketing of medical devices. Therefore, legal regulations do not release the specialists in the fields of medicine and dentistry from the responsibility to gather as much information as possible about the products used or to request this information from the manufacturer. Safety data sheets for medical devices can be downloaded from the Internet. They are an important source of information about the biocompatibility of dental materials as they were investigated by the manufacturers. Appropriate safety labels on the wrappings should be considered. The manufacturer/importer is responsible for its products and is potentially liable for damages. The medical doctors and dentists should use only those medical devices for which appropriate information is available.

  11. Structural and biocompatible characterization of TiC/a:C nanocomposite thin films.

    Science.gov (United States)

    Balázsi, K; Vandrovcová, M; Bačáková, L; Balázsi, Cs

    2013-04-01

    In this work, sputtered TiC/amorphous C thin films have been developed in order to be applied as potential barrier coating for interfering of Ti ions from pure Ti or Ti alloy implants. Our experiments were based on magnetron sputtering method, because the vacuum deposition provides great flexibility for manipulating material chemistry and structure, leading to films and coatings with special properties. The films have been deposited on silicon (001) substrates with 300 nm thick oxidized silicon sublayer at 200 °C deposition temperature as model substrate. Transmission electron microscopy has been used for structural investigations. Thin films consisted of ~20 nm TiC columnar crystals embedded by 5 nm thin amorphous carbon matrix. MG63 osteoblast cells have been applied for in vitro study of TiC nanocomposites. The cell culture tests give strong evidence of thin films biocompatibility. PMID:23827622

  12. Buckling Instability of Dielectric Elastomeric Plates for Soft, Bio-Compatible Microfluidic Pumps

    Science.gov (United States)

    Tavakol, Behrouz; Bozlar, Michael; Froehlicher, Guillaume; Punckt, Christian; Stone, Howard A.; Aksay, Ilhan; Holmes, Douglas

    2013-03-01

    Dielectric elastomers are well-known for their superior stretchability and permittivity. A fully-clamped thin elastomer will buckle when it is compressed by applying sufficient electric potentials to its sides. When embedded within soft, silicone rubbers, these advanced materials can provide a means for a bio-compatible pumping mechanism that can be used to inject bio-fluids with desired flow rates into microfluidic devices, tissues, and organs of interest. We have incorporated a dielectric film that is sandwiched between two thin, flexible, solid electrodes into a microfluidic device and utilized a voltage-induced out-of-plane buckling instability for pumping of fluids. We experimentally quantify the voltage-induced plate buckling and measure the fluid flow rate when the structure is embedded in a microchannel. Additionally, we offer an analytical prediction that uses plate buckling theory to estimate the flow rate as a function of applied voltage.

  13. In vitro biocompatibility of amorphous carbon based coatings by varying of surface chemistry and nitrogen concentrations

    International Nuclear Information System (INIS)

    The study of a-C:N coatings at different concentration of nitrogen, their surface chemistry and wettability effect on cell/material response in vitro test was performed. The surface structure of deposited coatings was investigated by means of scanning electron microscopy (SEM) and atomic force microscopy (AFM) methods. The coatings were characterized with respect to their bonding structure by photoelectron spectroscopy (XPS) analysis. The wettability was analysed by means of advanced water contact angle method and the surface free energy (SFE) was calculated according to Robertson equation. The biocompatibility was estimated by standard protocols. The best results were obtained in the case of coatings with the greater parameters of SFE and the minimal values of ratio N2 : C7H8

  14. Experimental investigation of plasma-immersion ion implantation treatment for biocompatible polyurethane implants production

    Science.gov (United States)

    Iziumov, R. I.; Beliaev, A. Y.; Kondyurina, I. V.; Shardakov, I. N.; Kondyurin, A. V.; Bilek, M. M.; McKenzie, D. R.

    2016-04-01

    Modification of the surface layer of polyurethane with plasma-immersion ion implantation (PIII) and studying its physical and chemical changes have been discussed in this paper. The goal of the research was to obtain carbonized layer allowing creating biocompatible polyurethane implants. The experiments of PIII treatment in various modes were performed. The investigation of the modified surface characteristics was carried out by observing the kinetics of free surface energy for two weeks after treatment. The regularities between treatment time and the level of free surface energy were detected. The explanation of high energy level was given through the appearance of free radicals in the surface layer of material. The confirmation of the chemical activation of the polyurethane surface after PIII treatment was obtained.

  15. Harnessing what lies within: Programming immunity with biocompatible devices to treat human disease

    Science.gov (United States)

    Roberts, Reid Austin

    Advances in our mechanistic insight of cellular function and how this relates to host physiology have revealed a world which is intimately connected at the macro and micro level. Our increasing understanding of biology exemplifies this, where cells respond to environmental cues through interconnected networks of proteins which function as receptors and adaptors to elicit gene expression changes that drive appropriate cellular programs for a given stimulus. Consequently, our deeper molecular appreciation of host homeostasis implicates aberrations of these pathways in nearly all major human disease categories, including those of infectious, metabolic, neurologic, oncogenic, and autoimmune etiology. We have come to recognize the mammalian immune system as a common network hub among all these varied pathologies. As such, the major goal of this dissertation is to identify a platform to program immune responses in mammals so that we may enhance our ability to treat disease and improve health in the 21st century. Using advances in materials science, in particular a recently developed particle fabrication technology termed Particle Replication in Non-wetting Templates (PRINT), our studies systematically assess the murine and human immune response to precisely fabricated nano- and microscale particles composed of biodegradable and biocompatible materials. We then build on these findings and present particle design parameters to program a number of clinically attractive immune responses by targeting endogenous cellular signaling pathways. These include control of particle uptake through surface modification, design parameters that modulate the magnitude and kinetics of biological signaling dynamics that can be used to exacerbate or dampen inflammatory responses, as well as particle designs which may be of use in treating allergies and autoimmune disorders. In total, this dissertation provides evidence that rational design of biocompatible nano- and microparticles is a viable

  16. Biocompatible magnetic core-shell nanocomposites for engineered magnetic tissues

    Science.gov (United States)

    Rodriguez-Arco, Laura; Rodriguez, Ismael A.; Carriel, Victor; Bonhome-Espinosa, Ana B.; Campos, Fernando; Kuzhir, Pavel; Duran, Juan D. G.; Lopez-Lopez, Modesto T.

    2016-04-01

    The inclusion of magnetic nanoparticles into biopolymer matrixes enables the preparation of magnetic field-responsive engineered tissues. Here we describe a synthetic route to prepare biocompatible core-shell nanostructures consisting of a polymeric core and a magnetic shell, which are used for this purpose. We show that using a core-shell architecture is doubly advantageous. First, gravitational settling for core-shell nanocomposites is slower because of the reduction of the composite average density connected to the light polymer core. Second, the magnetic response of core-shell nanocomposites can be tuned by changing the thickness of the magnetic layer. The incorporation of the composites into biopolymer hydrogels containing cells results in magnetic field-responsive engineered tissues whose mechanical properties can be controlled by external magnetic forces. Indeed, we obtain a significant increase of the viscoelastic moduli of the engineered tissues when exposed to an external magnetic field. Because the composites are functionalized with polyethylene glycol, the prepared bio-artificial tissue-like constructs also display excellent ex vivo cell viability and proliferation. When implanted in vivo, the engineered tissues show good biocompatibility and outstanding interaction with the host tissue. Actually, they only cause a localized transitory inflammatory reaction at the implantation site, without any effect on other organs. Altogether, our results suggest that the inclusion of magnetic core-shell nanocomposites into biomaterials would enable tissue engineering of artificial substitutes whose mechanical properties could be tuned to match those of the potential target tissue. In a wider perspective, the good biocompatibility and magnetic behavior of the composites could be beneficial for many other applications.The inclusion of magnetic nanoparticles into biopolymer matrixes enables the preparation of magnetic field-responsive engineered tissues. Here we

  17. A capillary viscometer designed for the characterization of biocompatible ferrofluids

    Science.gov (United States)

    Nowak, J.; Odenbach, S.

    2016-08-01

    Suspensions of magnetic nanoparticles are receiving a growing interest in biomedical research. These ferrofluids can, e.g., be used for the treatment of cancer, making use of the drug targeting principle or using an artificially induced heating. To enable a safe application the basic properties of the ferrofluids have to be well understood, including the viscosity of the fluids if an external magnetic field is applied. It is well known that the viscosity of ferrofluids rises if a magnetic field is applied, where the rise depends on shear rate and magnetic field strength. In case of biocompatible ferrofluids such investigations proved to be rather complicated as the experimental setup should be close to the actual application to allow justified predictions of the effects which have to be expected. Thus a capillary viscometer, providing a flow situation comparable to the flow in a blood vessel, has been designed. The glass capillary is exchangeable and different inner diameters can be used. The range of the shear rates has been adapted to the range found in the human organism. The application of an external magnetic field is enabled with two different coil setups covering the ranges of magnetic field strengths required on the one hand for a theoretical understanding of particle interaction and resulting changes in viscosity and on the other hand for values necessary for a potential biomedical application. The results show that the newly designed capillary viscometer is suitable to measure the magnetoviscous effect in biocompatible ferrofluids and that the results appear to be consistent with data measured with rotational rheometry. In addition, a strong change of the flow behaviour of a biocompatible ferrofluid was proven for ranges of the shear rate and the magnetic field strength expected for a potential biomedical application.

  18. A rheological and microscopical characterization of biocompatible ferrofluids

    Energy Technology Data Exchange (ETDEWEB)

    Nowak, J., E-mail: johannes.nowak@tu-dresden.de [Chair of Magnetofluiddynamics, Measuring and Automation Technology, Technische Universität Dresden, 01069 Germany (Germany); Wolf, D. [Triebenberg Laboratory, Technische Universität Dresden, 01328 Germany (Germany); Odenbach, S. [Chair of Magnetofluiddynamics, Measuring and Automation Technology, Technische Universität Dresden, 01069 Germany (Germany)

    2014-03-15

    There is an increasing interest in suspensions of magnetic nanoparticles in the biomedical area. Those ferrofluids are e.g. used for magnetic resonance imaging and emerging research focuses on employing the fluids for magnetic drug targeting or magnetic particle heating as a potential treatment for cancer. For these applications the knowledge of the suspensions' thermophysical properties is of major interest to guarantee a safe and effective application. Therefore the flow behavior cannot be neglected as it might significantly influence the execution of the aforementioned applications. In this experimental study two biocompatible ferrofluids were investigated. Rheological measurements were carried out using rotational rheometry. To allow an interpretation of the fluids' behavior the microscopic make-up was investigated using dynamic light scattering and transmission electron microscopy. Measurements of diluted ferrofluids were carried out as a first step to simulate the rheological behavior reflecting the concentration of magnetic nanoparticles found in blood flow for most biomedical applications of such fluids. The detected strong effects show the potential to significantly influence application and handling of the biocompatible ferrofluids in the medical area and should therefore be taken into account for further research as well as for the application of such fluids. - Highlights: • The rheology of biocompatible multicore ferrofluids is influenced by magnetic fields. • The flow curves can be described by the Herschel–Bulkley model. • A connection between the magnetoviscous effect and the particle size is found. • The strong magnetoviscous effect exists even if the fluids are diluted. • The connection between the effect and the dilution is mathematically described.

  19. A rheological and microscopical characterization of biocompatible ferrofluids

    International Nuclear Information System (INIS)

    There is an increasing interest in suspensions of magnetic nanoparticles in the biomedical area. Those ferrofluids are e.g. used for magnetic resonance imaging and emerging research focuses on employing the fluids for magnetic drug targeting or magnetic particle heating as a potential treatment for cancer. For these applications the knowledge of the suspensions' thermophysical properties is of major interest to guarantee a safe and effective application. Therefore the flow behavior cannot be neglected as it might significantly influence the execution of the aforementioned applications. In this experimental study two biocompatible ferrofluids were investigated. Rheological measurements were carried out using rotational rheometry. To allow an interpretation of the fluids' behavior the microscopic make-up was investigated using dynamic light scattering and transmission electron microscopy. Measurements of diluted ferrofluids were carried out as a first step to simulate the rheological behavior reflecting the concentration of magnetic nanoparticles found in blood flow for most biomedical applications of such fluids. The detected strong effects show the potential to significantly influence application and handling of the biocompatible ferrofluids in the medical area and should therefore be taken into account for further research as well as for the application of such fluids. - Highlights: • The rheology of biocompatible multicore ferrofluids is influenced by magnetic fields. • The flow curves can be described by the Herschel–Bulkley model. • A connection between the magnetoviscous effect and the particle size is found. • The strong magnetoviscous effect exists even if the fluids are diluted. • The connection between the effect and the dilution is mathematically described

  20. Exploration of New Electroacupuncture Needle Material

    OpenAIRE

    Sanghun Lee; Gwang-Ho Choi; Chang Hoon Lee; Yu Kyoung Kim; Saebhom Lee; Sungjin Cho; Sunhee Yeon; Sun-Mi Choi; Yeon-Hee Ryu

    2012-01-01

    Background. Electro Acupuncture (EA) uses the acupuncture needle as an electrode to apply low-frequency stimulation. For its safe operation, it is essential to prevent any corrosion of the acupuncture needle. Objective. The aim of this study is to find an available material and determine the possibility of producing a standard EA needle that is biocompatible. Methods. Biocompatibility was tested by an MTT assay and cytotoxicity testing. Corrosion was observed with a scanning electron microsco...

  1. Model Operation of the Strength Properties of Ventplant Biocompatible Coatings

    Directory of Open Access Journals (Sweden)

    Lyasnikova A.V.

    2013-09-01

    Full Text Available A special porous coating is applied on the surface of dental implants to improve it`s biocompatibility. During the maintenance the tensions originate in such coatings, which is added to the residual pressure and significantly reduce the strength of the coatings. It is almost impossible to get the numericalled value of these tensions experimentally, so in this article we solve the problem of mathematical model operation of the tension originating in porous coatings in the process of their forming, and in the course of maintenance

  2. Corrigendum: Softec HD hydrophilic acrylic intraocular lens: biocompatibility and precision

    Directory of Open Access Journals (Sweden)

    Espandar L

    2011-02-01

    Full Text Available Espandar L, Sikder S, Moshirfar M. Softec HD hydrophilic acrylic intraocular lens: biocompatibility and precision. Clin Ophthalmol. 2011;5:65–70.There was an error in the reported optic size of Lenstec’s Softec HD intraocular lens, which was reported to be 5.5 mm in Espandar et al’s work, but is 5.75 mm.Please see the specifications on Lenstec’s Web site for further details (see http://www.lenstec.com/lenstec/hd_specs.html.Read the original article

  3. Biocompatible implants and methods of making and attaching the same

    Energy Technology Data Exchange (ETDEWEB)

    Rowley, Adrian P; Laude, Lucien D; Humayun, Mark S; Weiland, James D; Lotfi, Atoosa; Markland, Jr., Francis S

    2014-10-07

    The invention provides a biocompatible silicone implant that can be securely affixed to living tissue through interaction with integral membrane proteins (integrins). A silicone article containing a laser-activated surface is utilized to make the implant. One example is an implantable prosthesis to treat blindness caused by outer retinal degenerative diseases. The device bypasses damaged photoreceptors and electrically stimulates the undamaged neurons of the retina. Electrical stimulation is achieved using a silicone microelectrode array (MEA). A safe, protein adhesive is used in attaching the MEA to the retinal surface and assist in alleviating focal pressure effects. Methods of making and attaching such implants are also provided.

  4. Biodegradable Xylitol-Based Elastomers: In Vivo Behavior and Biocompatibility

    Science.gov (United States)

    Bruggeman, Joost P.; Bettinger, Christopher J.; Langer, Robert

    2010-01-01

    Biodegradable elastomers based on polycondensation reactions of xylitol with sebacic acid, referred to as poly(xylitol sebacate) (PXS) elastomers have recently been developed. Herein, we describe the in vivo behavior of PXS elastomers. Four PXS elastomers were synthesized, characterized and compared to poly(L-lactic-co-glycolic acid) (PLGA). PXS elastomers displayed a high level of structural integrity and form stability during degradation. The in vivo half-life ranged from approximately 3 to 52 weeks. PXS elastomers exhibited increased biocompatibility compared to PLGA implants. PMID:20540093

  5. Biocompatibility of phosphorylcholine coated stents in normal porcine coronary arteries

    OpenAIRE

    Whelan, Deirdre; Krabbendam, S.C.; Verdouw, Pieter; Serruys, Patrick; van Vliet, Erwin; Giessen, Wim; Van Beusekom, Heleen

    2000-01-01

    OBJECTIVE—To improve the biocompatibility of stents using a phosphorylcholine coated stent as a form of biomimicry.
INTERVENTIONS—Implantation of phosphorylcholine coated (n = 20) and non-coated (n = 21) stents was performed in the coronary arteries of 25 pigs. The animals were killed after five days (n = 6), four weeks (n = 7), and 12 weeks (n = 8), and the vessels harvested for histology, scanning electron microscopy, and morphometry.
MAIN OUTCOME MEASURES—Stent performance was assessed by ...

  6. Plasma polymerized carvone as an antibacterial and biocompatible coating.

    Science.gov (United States)

    Chan, Yuen Wah; Siow, Kim Shyong; Ng, Pei Yuen; Gires, Usup; Yeop Majlis, Burhanuddin

    2016-11-01

    Antibacterial coating is important to prevent the colonization of medical devices by biofilm forming bacteria that would cause infection and sepsis in patients. Current coating techniques such as immobilization of antimicrobial compounds, time-releasing antibiotic agents and silver nanoparticles, require multiple processing steps, and they have low efficacy and low stability. We proposed a single-step plasma polymerization of an essential oil known as carvone to produce a moderately hydrophobic antibacterial coating (ppCar) with an average roughness of cell line. This study would be of interest to researcher keen on producing a bacteria-resistance and biocompatible coating on different substrates in a cost-effective manner. PMID:27524089

  7. Surface modification of polymers for biocompatibility via exposure to extreme ultraviolet radiation

    OpenAIRE

    Ahad, I.U.; Bartnik, A.; Fiedorowicz, Henryk; Kostecki, Jerzy; Korkzyc, Barbara; Ciach, Tomasz; Brabazon, Dermot

    2013-01-01

    Polymeric biomaterials are being widely used for the treatment of various traumata, diseases and defects in human beings due to ease in their synthesis. As biomaterials have direct interaction with the extracellular environment in the biological world, biocompatibility is a topic of great significance. The introduction or enhancement of biocompatibility in certain polymers is still a challenge to overcome. Polymer biocompatibility can be controlled by surface modification. Various physical an...

  8. Enhanced biocompatibility for plasmid DNA on patterned TiO2 surfaces

    Science.gov (United States)

    Majumder, Subrata; Mishra, I.; Subudhi, U.; Varma, Shikha

    2013-08-01

    An enhanced biocompatibility from nanodot patterned TiO2 surfaces, fabricated by ion beam sputtering, has been observed here through its interaction with plasmid DNA. Investigations of the persistence length and the areal conformation of DNA show that the biocompatibility increases with ion fluence. Presence of nanostructures and increased surface roughness, in conjugation with higher oxygen vacancy sites that promote charge transfer from DNA moiety, are responsible for the increased hydrophilicity and biocompatibility of the patterned surfaces.

  9. Biocompatible post-polymerization functionalization of a water soluble poly(p-phenylene ethynylene)

    OpenAIRE

    Swager, Timothy Manning; Vanveller, Brett Steven

    2010-01-01

    A biocompatible post-polymerization functionalization reaction takes advantage of a polymer's structural motif for the controllable attachment of biotin as a model biosensor that responds to streptavidin.

  10. A novel smart injectable hydrogel prepared by microbial transglutaminase and human-like collagen: Its characterization and biocompatibility.

    Science.gov (United States)

    Zhao, Leilei; Li, Xian; Zhao, Jiaqi; Ma, Saijian; Ma, Xiaoxuan; Fan, Daidi; Zhu, Chenhui; Liu, Yannan

    2016-11-01

    Various tissue scaffold materials are increasingly used to repair skin defects by cross-linking because of the ability to fill and implant in any form via operation. However, crosslinker residues cannot be easily removed from scaffold materials prepared by chemical crosslinking methods, limiting their use for skin tissue engineering. Here, microbial transglutaminase (MTGase), a nontoxic crosslinker with high specific activity and reaction rate under mild conditions, was employed crosslinks in human-like collagen (HLC) to yield novel smart MTGase crosslinked with human-like collagen (MTGH) hydrogels, which are sensitive to temperature and/or enzymes. Various ratios of MTGase/HLC were performed, and their physicochemical properties were characterized, including the swelling ratio, the elastic modulus, the morphology and the porosity. The degradation behavior and mechanism of MTGase in concentration-dependent manner involved in formation hydrogels were identifying in vitro. The cell attachment in vitro and biocompatibility in vivo were also investigated. The results demonstrated that the use of different concentrations of MTGase to crosslink HLC produced products with different degradation times and biocompatibilities. The 50U/g MTGase-prepared MTGH hydrogels had a higher density of crosslinks, which made them more resistant to degradation by collagenase I and collagenase II. However, 40U/g MTGase-prepared MTGH hydrogels were more suitable for cell attachment. In addition, compared with the Collagen Implant I® (SUM) used in animal experiments, the 40U/g MTGase-prepared MTGH hydrogels had a lower toxicity and better biocompatibility. Therefore, 40U/g MTGase crosslinked with HLC should be used to prepare MTGH hydrogels for potential application as soft materials for skin tissue engineering. PMID:27524026

  11. Safety and biocompatibility of graphene: A new generation nanomaterial for biomedical application.

    Science.gov (United States)

    Syama, S; Mohanan, P V

    2016-05-01

    Graphene, a material with great application potential is expected to revolutionize various fields in the near future particularly biomedical field with its inherent properties. However, significant increase in the research on graphene in the recent years has created anxiety about their safety/biocompatibility towards living organisms. Though there is increase in reports on graphene synthesis and application, in parallel reports on unwanted toxic effects of these materials is under scrutiny. Before exploiting their use, any engineered nanomaterials should undergo through investigation regarding the risk and health hazards imposed by them. Toxicity of nanomaterial depends on many factors like size, shape, surface chemistry, dose, duration and the biological milieu. In this account, we reviewed physico-chemical properties of graphene that plays a key role in toxicity prediction. We also detailed some examples of the in vitro and in vivo toxicity studies that have been published so far. The potential environmental risk associated with these carbon materials is also addressed, in order to avoid unintentional leaching of these materials into surface water. PMID:26851208

  12. Biocompatibility assessment of spark plasma-sintered alumina-titanium cermets.

    Science.gov (United States)

    Guzman, Rodrigo; Fernandez-García, Elisa; Gutierrez-Gonzalez, Carlos F; Fernandez, Adolfo; Lopez-Lacomba, Jose Luis; Lopez-Esteban, Sonia

    2016-01-01

    Alumina-titanium materials (cermets) of enhanced mechanical properties have been lately developed. In this work, physical properties such as electrical conductivity and the crystalline phases in the bulk material are evaluated. As these new cermets manufactured by spark plasma sintering may have potential application for hard tissue replacements, their biocompatibility needs to be evaluated. Thus, this research aims to study the cytocompatibility of a novel alumina-titanium (25 vol. % Ti) cermet compared to its pure counterpart, the spark plasma sintered alumina. The influence of the particular surface properties (chemical composition, roughness and wettability) on the pre-osteoblastic cell response is also analyzed. The material electrical resistance revealed that this cermet may be machined to any shape by electroerosion. The investigated specimens had a slightly undulated topography, with a roughness pattern that had similar morphology in all orientations (isotropic roughness) and a sub-micrometric average roughness. Differences in skewness that implied valley-like structures in the cermet and predominance of peaks in alumina were found. The cermet presented a higher surface hydrophilicity than alumina. Any cytotoxicity risk associated with the new materials or with the innovative manufacturing methodology was rejected. Proliferation and early-differentiation stages of osteoblasts were statistically improved on the composite. Thus, our results suggest that this new multifunctional cermet could improve current alumina-based biomedical devices for applications such as hip joint replacements. PMID:25956565

  13. In vitro Biocompatibility of New Silver(I Coordination Compound Coated-Surfaces for Dental Implant Applications

    Directory of Open Access Journals (Sweden)

    Priscilla S. Brunetto

    2011-01-01

    Full Text Available Biofilm formation on implant materials causes a common problem: resistance to aggressive pharmacological agents as well as host defenses. Therefore, to reduce bacterial adhesion to implant surfaces we propose to use silver(I coordination networks as it is known that silver is the most powerful antimicrobial inorganic agent. As a model surface, self-assembled monolayers (SAMs on gold Au(111 was used to permit permanent attachment of our silver(I coordination networks. The surface coatings showed typical nano-structured surfaces with a good biocompatibility for soft-tissue integration with fibroblast cells.

  14. Biocompatibility of nanoactuators: stem cell growth on laser-generated nickel-titanium shape memory alloy nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Barcikowski, Stephan, E-mail: s.barcikowski@lzh.de; Hahn, Anne [Laser Zentrum Hannover e.V. (Germany); Guggenheim, Merlin; Reimers, Kerstin [Medical School Hannover, Department of Plastic, Hand and Reconstructive Surgery (Germany); Ostendorf, Andreas [Laser Zentrum Hannover e.V. (Germany)

    2010-06-15

    Nanoactuators made from nanoparticulate NiTi shape memory alloy show potential in the mechanical stimulation of bone tissue formation from stem cells. We demonstrate the fabrication of Ni, Ti, and NiTi shape memory alloy nanoparticles and their biocompatibility to human adipose-derived stem cells. The stoichiometry and phase transformation property of the bulk alloy is preserved during attrition by femtosecond laser ablation in liquid, giving access to colloidal nanoactuators. No adverse effect on cell growth and attachment is observed in proliferation assay and environmental electron scanning microscopy, making this material attractive for mechanical stimulation of stem cells.

  15. Biocompatibility of nanoactuators: stem cell growth on laser-generated nickel-titanium shape memory alloy nanoparticles

    International Nuclear Information System (INIS)

    Nanoactuators made from nanoparticulate NiTi shape memory alloy show potential in the mechanical stimulation of bone tissue formation from stem cells. We demonstrate the fabrication of Ni, Ti, and NiTi shape memory alloy nanoparticles and their biocompatibility to human adipose-derived stem cells. The stoichiometry and phase transformation property of the bulk alloy is preserved during attrition by femtosecond laser ablation in liquid, giving access to colloidal nanoactuators. No adverse effect on cell growth and attachment is observed in proliferation assay and environmental electron scanning microscopy, making this material attractive for mechanical stimulation of stem cells.

  16. Evaluation of biocompatibility of a new root canal irrigant Q Mix TM 2 in 1- An in vivo study

    OpenAIRE

    Veeramachaneni Chandrasekhar; Vanapatla Amulya; Vanapatla Swaroopa Rani; T Jaya Prakash; A Siva Ranjani; Ch Gayathri

    2013-01-01

    Aim: To evaluate the biocompatibility of a new root canal irrigant Q mix™ 2 in 1 in comparison to 0.9% sterile saline, 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 17% ethylenediaminetetraacetic acid (EDTA). Materials and Methods: Six circles were drawn on the dorsal skin of 24 male Wistar Albino rats, leaving 2cm between each circle. Using a syringe, 0.1mL of each root canal irrigant was injected subcutaneously into five circles. In the sixth circle, the needle of an empty...

  17. Biocompatible and target specific hydrophobically modified glycol chitosan nanoparticles.

    Science.gov (United States)

    Yin, Wei; Li, Weiyi; Rubenstein, David A; Meng, Yizhi

    2016-01-01

    Cardiovascular disease is the leading cause of death in the United States. Atherosclerosis is a major cause for cardiovascular diseases. Drugs that treat atherosclerosis usually act nonspecifically. To enhance drug delivery specificity, the authors developed a hydrophobically modified glycol chitosan (HGC) nanoparticle that can specifically target activated endothelial cells. The biocompatibility of these nanoparticles toward red blood cells and platelets was evaluated through hemolysis, platelet activation, platelet thrombogenicity, and platelet aggregation assays. The biocompatibility of these nanoparticles toward vascular endothelial cells was evaluated by their effects on endothelial cell growth, metabolic activity, and activation. The results demonstrated that HGC nanoparticles did not cause hemolysis, or affect platelet activation, thrombogenicity, and aggregation capability in vitro. The nanoparticles did not impair vascular endothelial cell growth or metabolic activities in vitro, and did not cause cell activation either. When conjugated with intercellular adhesion molecular 1 antibodies, HGC nanoparticles showed a significantly increased targeting specificity toward activated endothelial cells. These results suggested that HGC nanoparticles are likely compatible toward red blood cells, platelets, and endothelial cells, and they can be potentially used to identify activated endothelial cells at atherosclerotic lesion areas within the vasculature, and deliver therapeutic drugs. PMID:27126597

  18. Biocompatibility evaluation of magnetosomes formed by Acidithiobacillus ferrooxidans

    Energy Technology Data Exchange (ETDEWEB)

    Yan Lei [Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, 730000 (China); College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319 (China); Yue Xiaoxuan [Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, 730000 (China); Zhang Shuang [College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319 (China); Chen Peng [Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, 730000 (China); GIBT, Gansu Institute of Business and Technology, Yannan Road 18, Lanzhou, 730010 (China); Xu Zhiliang; Li Yang [Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, 730000 (China); Li Hongyu, E-mail: hekouyanlei@gmail.com [Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, 730000 (China)

    2012-10-01

    Magnetite nanocrystal has been extensively used in biomedical field. Currently, an interesting alternative to synthetic magnetic Fe{sub 3}O{sub 4} nanoparticles, called magnetosome, has been found in magnetotactic bacteria. It has been reported that Acidithiobacillus ferrooxidans (At. ferrooxidans) has a potential to synthesize magnetosome. In this study, transmission electron microscope (TEM) was used to analyze the magnetite particles in At. ferrooxidans BY-3. The magnetosomes formed by this bacterium were isolated by a method combining ultracentrifugation and magnetic separation. Crystalline phase and surface functional group of the magnetosomes were investigated by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Biocompatibility of the magnetosomes was systematically evaluated at various concentrations (0.5, 1.0, 2.0 and 4.0 mg/ml). MTT test, hemolysis assay and Micronucleus Test were carried out to evaluate in vitro cytotoxicity, blood toxicity and genotoxicity of magnetosomes, respectively. Under these conditions, magnetosomes showed no cytotoxic, genotoxic and hemolytic effects up to 4.0 mg/ml indicating good biocompatibility of these biological nanoparticles. These revealed that the magnetosomes might have a potential for biotechnological and biomedical applications in the future. - Highlights: Black-Right-Pointing-Pointer The production of magnetosomes from At. ferrooxidans has been easily available. Black-Right-Pointing-Pointer Several techniques are used to characterize properties of the magnetosomes. Black-Right-Pointing-Pointer The magnetosomes have no cytotoxicity, no hemolysis activity and no genotoxicity.

  19. Dispersion of multi-walled carbon nanotubes in biocompatible dispersants

    International Nuclear Information System (INIS)

    Owing to their phenomenal electrical and mechanical properties, carbon nanotubes (CNT) have been an area of intense research since their discovery in 1991. Different applications for these nanoparticles have been proposed, among others, in electronics and optics but also in the medical field. In parallel, emerging studies have suggested potential toxic effects of CNT while others did not, generating some conflicting outcomes. These discrepancies could be, in part, due to different suspension approaches used and to the agglomeration state of CNT in solution. In this study, we described a standardized protocol to obtain stable CNT suspensions, using two biocompatible dispersants (Pluronic F108 and hydroxypropylcellulose) and to estimate the concentration of CNT in solution. CNT appear to be greatly individualized in these two dispersants with no detection of remaining bundles or agglomerates after sonication and centrifugation. Moreover, CNT remained perfectly dispersed when added to culture medium used for in vitro cell experiments. We also showed that Pluronic F108 is a better dispersant than hydroxypropylcellulose. In conclusion, we have developed a standardized protocol using biocompatible surfactants to obtain reproducible and stable multi-walled carbon nanotubes suspensions which can be used for in vitro or in vivo toxicological studies.

  20. Biocompatible fluorescent nanoparticles for in vivo stem cell tracking

    International Nuclear Information System (INIS)

    Efficient application of stem cells to the treatment of neurodegenerative diseases requires safe cell tracking to follow stem cell fate over time in the host environment after transplantation. In this work, for the first time, fluorescent and biocompatible methyl methacrylate (MMA)-based nanoparticles (fluoNPs) were synthesized through a free-radical co-polymerization process with a fluorescent macromonomer obtained by linking Rhodamine B and hydroxyethyl methacrylate. We demonstrate that the fluoNPs produced by polymerization of MMA–Rhodamine complexes (1) were efficient for the labeling and tracking of multipotent human amniotic fluid cells (hAFCs); (2) did not alter the main biological features of hAFCs (such as viability, cell growth and metabolic activity); (3) enabled us to determine the longitudinal bio-distribution of hAFCs in different brain areas after graft in the brain ventricles of healthy mice by a direct fluorescence-based technique. The reliability of our approach was furthermore confirmed by magnetic resonance imaging analyses, carried out by incubating hAFCs with both superparamagnetic iron oxide nanoparticles and fluoNPs. Our data suggest that these finely tunable and biocompatible fluoNPs can be exploited for the longitudinal tracking of stem cells. (paper)

  1. Biocompatibility evaluation of magnetosomes formed by Acidithiobacillus ferrooxidans

    International Nuclear Information System (INIS)

    Magnetite nanocrystal has been extensively used in biomedical field. Currently, an interesting alternative to synthetic magnetic Fe3O4 nanoparticles, called magnetosome, has been found in magnetotactic bacteria. It has been reported that Acidithiobacillus ferrooxidans (At. ferrooxidans) has a potential to synthesize magnetosome. In this study, transmission electron microscope (TEM) was used to analyze the magnetite particles in At. ferrooxidans BY-3. The magnetosomes formed by this bacterium were isolated by a method combining ultracentrifugation and magnetic separation. Crystalline phase and surface functional group of the magnetosomes were investigated by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Biocompatibility of the magnetosomes was systematically evaluated at various concentrations (0.5, 1.0, 2.0 and 4.0 mg/ml). MTT test, hemolysis assay and Micronucleus Test were carried out to evaluate in vitro cytotoxicity, blood toxicity and genotoxicity of magnetosomes, respectively. Under these conditions, magnetosomes showed no cytotoxic, genotoxic and hemolytic effects up to 4.0 mg/ml indicating good biocompatibility of these biological nanoparticles. These revealed that the magnetosomes might have a potential for biotechnological and biomedical applications in the future. - Highlights: ► The production of magnetosomes from At. ferrooxidans has been easily available. ► Several techniques are used to characterize properties of the magnetosomes. ► The magnetosomes have no cytotoxicity, no hemolysis activity and no genotoxicity.

  2. Functionally graded hydroxyapatite-alumina-zirconia biocomposite: Synergy of toughness and biocompatibility

    Energy Technology Data Exchange (ETDEWEB)

    Afzal, Mohammad Atif Faiz [Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur (India); Kesarwani, Pallavi [Visvesvaraya National Institute of Technology Nagpur (India); Reddy, K. Madhav; Kalmodia, Sushma [Laboratory for Biomaterials, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur (India); Basu, Bikramjit [Materials Research Centre, Indian Institute of Science, Bangalore-560012 (India); Balani, Kantesh, E-mail: kbalani@iitk.ac.in [Biomaterials Processing and Characterization Laboratory, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur (India); Laboratory for Biomaterials, Department of Materials Science and Engineering, Indian Institute of Technology Kanpur (India)

    2012-07-01

    Functionally Gradient Materials (FGM) are considered as a novel concept to implement graded functionality that otherwise cannot be achieved by conventional homogeneous materials. For biomedical applications, an ideal combination of bioactivity on the material surface as well as good physical property (strength/toughness/hardness) of the bulk is required in a designed FGM structure. In this perspective, the present work aims at providing a smooth gradation of functionality (enhanced toughening of the bulk, and retained biocompatibility of the surface) in a spark plasma processed hydroxyapatite-alumina-zirconia (HAp-Al{sub 2}O{sub 3}-YSZ) FGM bio-composite. In the current work HAp (fracture toughness {approx} 1.5 MPa.m{sup 1/2}) and YSZ (fracture toughness {approx} 6.2 MPa.m{sup 1/2}) are coupled with a transition layer of Al{sub 2}O{sub 3} allowing minimum gradient of mechanical properties (especially the fracture toughness {approx} 3.5 MPa.m{sup 1/2}). The in vitro cyto-compatibilty of HAp-Al{sub 2}O{sub 3}-YSZ FGM was evaluated using L929 fibroblast cells and Saos-2 Osteoblast cells for their adhesion and growth. From analysis of the cell viability data, it is evident that FGM supports good cell proliferation after 2, 3, 4 days culture. The measured variation in hardness, fracture toughness and cellular adhesion across the cross section confirmed the smooth transition achieved for the FGM (HAp-Al{sub 2}O{sub 3}-YSZ) nanocomposite, i.e. enhanced bulk toughness combined with unrestricted surface bioactivity. Therefore, such designed biomaterials can serve as potential bone implants. - Graphical abstract: Spark plasma sintered functionally gradient materials (FGM) eliciting the YSZ - Al{sub 2}O{sub 3}-YSZ and HAp-Al{sub 2}O{sub 3} interface with grading hardness, toughness and biocompatibility response. Highlights: Black-Right-Pointing-Pointer Development of functionally gradient material to serve as a potential bone implant. Black-Right-Pointing-Pointer Introduction

  3. Functionally graded hydroxyapatite-alumina-zirconia biocomposite: Synergy of toughness and biocompatibility

    International Nuclear Information System (INIS)

    Functionally Gradient Materials (FGM) are considered as a novel concept to implement graded functionality that otherwise cannot be achieved by conventional homogeneous materials. For biomedical applications, an ideal combination of bioactivity on the material surface as well as good physical property (strength/toughness/hardness) of the bulk is required in a designed FGM structure. In this perspective, the present work aims at providing a smooth gradation of functionality (enhanced toughening of the bulk, and retained biocompatibility of the surface) in a spark plasma processed hydroxyapatite-alumina-zirconia (HAp-Al2O3-YSZ) FGM bio-composite. In the current work HAp (fracture toughness ∼ 1.5 MPa.m1/2) and YSZ (fracture toughness ∼ 6.2 MPa.m1/2) are coupled with a transition layer of Al2O3 allowing minimum gradient of mechanical properties (especially the fracture toughness ∼ 3.5 MPa.m1/2). The in vitro cyto-compatibilty of HAp-Al2O3-YSZ FGM was evaluated using L929 fibroblast cells and Saos-2 Osteoblast cells for their adhesion and growth. From analysis of the cell viability data, it is evident that FGM supports good cell proliferation after 2, 3, 4 days culture. The measured variation in hardness, fracture toughness and cellular adhesion across the cross section confirmed the smooth transition achieved for the FGM (HAp-Al2O3-YSZ) nanocomposite, i.e. enhanced bulk toughness combined with unrestricted surface bioactivity. Therefore, such designed biomaterials can serve as potential bone implants. - Graphical abstract: Spark plasma sintered functionally gradient materials (FGM) eliciting the YSZ – Al2O3-YSZ and HAp-Al2O3 interface with grading hardness, toughness and biocompatibility response. Highlights: ► Development of functionally gradient material to serve as a potential bone implant. ► Introduction of Aluminum oxide and Yttria Stabilized Zirconia (YSZ) cushion layer. ► Smooth fracture toughness gradient between hydroxyapatite and YSZ interface.

  4. Apoferritin Nanoparticle: A Novel and Biocompatible Carrier for Enzyme Immobilization with Enhanced Activity and Stability

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Youyu; Tang, Zhiwen; Wang, Jun; Wu, Hong J.; Lin, Chiann Tso; Lin, Yuehe

    2011-11-01

    Apoferritin is a nanostructured material with a uniform size and spherical structure, and it has excellent bio-compatibility. In this work, we report the use of apoferritin as a novel and biocompatible carrier for stabilizing enzymes and their activities. We used glucose oxidase (GOx) as a model enzyme. GOx was immobilized on the surface of the apoferritin through a green synthetic approach taking advantage of bioaffinity binding between streptavidin and biotin. As a result, a glucose oxidase-biotin/streptavidin/biotin-apoferritin conjugate (Apo-GOx) was prepared using streptavidin as a bridge. The synthesized Apo-GOx was characterized with transmission electron microscopy, ultraviolet, and fluorescence spectroscopy. The activity and stability of GOx on the surface of the apoferritin were studied in different environments, such as temperature, chemicals, and pH, in comparison with the biotinylated GOx (B-GOx). The results showed that the activity of GOx on the apoferritin surface was significantly enhanced. The thermal and chemical stability of the GOx on the apoferritin was also greatly improved compared to free B-GOx in a solution. It was found that the activity of the GOx on the apoferritin only lost 30% in comparison to a 70% loss of free B-GOx after a 2 h incubation at 50oC. There was almost no decrease in activity for the GOx on the apoferritin as compared to an 80% activity decrease for free B-GOx after 30 min incubation in a 5 M urea solution. Glucose detection was used as a model application for the enzyme immobilization method developed in this work. The GOx immobilized apoferritin nanoparticles exhibited high sensitivity for glucose detection with a detection limit of 3 nM glucose. This work offers a novel approach for immobilizing enzymes with enhanced stability and activity, and this method may find a number of applications, such as in enzyme catalysis, DNA assays and immunoassays.

  5. Biocompatibility and biomineralization assessment of bioceramic-, epoxy-, and calcium hydroxide-based sealers.

    Science.gov (United States)

    Bueno, Carlos Roberto Emerenciano; Valentim, Diego; Marques, Vanessa Abreu Sanches; Gomes-Filho, João Eduardo; Cintra, Luciano Tavares Angelo; Jacinto, Rogério Castilho; Dezan-Junior, Eloi

    2016-06-14

    Obturation of the root canal system aims to fill empty spaces, promoting hermetic sealing and preventing bacterial activity in periapical tissues. This should provide optimal conditions for repair, stimulating the process of biomineralization. An endodontic sealer should be biocompatible once it is in direct contact with periapical tissues. The aim of this study was to evaluate the rat subcutaneous tissue response to implanted polyethylene tubes filled with Smartpaste Bio, Acroseal, and Sealapex and investigate mineralization ability of these endodontic sealers. Forty Wistar rats were assigned to the three sealers groups and control group, (n = 10 animals/group) and received subcutaneous implants containing the test sealers, and the control group were implanted with empty tubes. After days 7, 15, 30, and 60, animals were euthanized and polyethylene tubes were removed with the surrounding tissues. Inflammatory infiltrate and thickness of the fibrous capsule were histologically evaluated. Mineralization was analyzed by Von Kossa staining and polarized light. Data were tabulated and analyzed via Kruskal-Wallis and Dunn's test. All tested materials induced a moderate inflammatory reaction in the initial periods. Smartpaste Bio induced the mildest inflammatory reactions after day 15. No difference was observed among groups after days 30 or 60. Von Kossa-positive staining and birefringent structures observed under polarized light revealed a larger mineralization area in Sealapex-treated animals followed by Smartpaste Bio-treated animals. At the end of the experiment, all tested sealers were found to be biocompatible. All sealers induced biomineralization, except Acroseal, which induced a mild tissue reaction. PMID:27305513

  6. Biocompatibility and degradation of gold-covered magneto-elastic biosensors exposed to cell culture.

    Science.gov (United States)

    Menti, C; Beltrami, M; Possan, A L; Martins, S T; Henriques, J A P; Santos, A D; Missell, F P; Roesch-Ely, M

    2016-07-01

    Magneto-elastic materials (ME) have important advantages when applied as biosensors due to the possibility of wireless monitoring. Commercial Metglas 2826MB3™ (FeNiMoB) is widely used, however sensor stabilization is an important factor for biosensor performance. This study compared the effects of biocompatibility and degradation of the Metglas 2826MB3™ alloy, covered or not with a gold layer, when in contact with cell culture medium. Strips of amorphous Metglas 2826MB3™ were cut and coated with thin layers of Cr and Au, as verified by Rutherford Backscattering Spectroscopy (RBS). Using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES), the presence of metals in the culture medium was quantitatively determined for up to seven days after alloy exposure. Biocompatibility of fibroblast Chinese Hamster Ovary (CHO) cultures was tested and cytotoxicity parameters were investigated by indirect means of reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) at 1, 2 and 7 days. Cell death was further evaluated through in situ analysis using Acridine Orange/Ethidium Bromide (AO/EB) staining and images were processed with ImageJ software. Ions from Metglas(®) 2826MB3™ induced a degradation process in living organisms. The cytotoxicity assay showed a decrease in the percentage of live cells compared to control for the ME strip not coated with gold. AO/EB in situ staining revealed that most of the cells grown on top of the gold-covered sensor presented a normal morphology (85.46%). Covering ME sensors with a gold coating improved their effectiveness by generating protection of the transducer by reducing the release of ions and promoting a significant cell survival. PMID:26998872

  7. Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane

    Directory of Open Access Journals (Sweden)

    Yili Qu

    2010-06-01

    Full Text Available Yili Qu1,3, Ping Wang1,3, Yi Man1, Yubao Li2, Yi Zuo2, Jidong Li21State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064, China; 2Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, China; 3These authors contributed equally to this workAbstract: Nano-hydroxyapatite/polyamide 66 (nHA/PA66 composite with good bioactivity and osteoconductivity was employed to develop a novel porous membrane with asymmetric structure for guided bone regeneration (GBR. In order to test material cytotoxicity and to investigate surface-dependent responses of bone-forming cells, the morphology, proliferation, and cell cycle of bone marrow stromal cells (BMSCs of rats cultured on the prepared membrane were determined. The polygonal and fusiform shape of BMSCs was observed by scanning electronic microscopy (SEM. The proliferation of BMSCs cultured on nHA/PA66 membrane tested by the MTT method (MTT: [3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazoliumbromide] was higher than that of negative control groups for 1 and 4 days’ incubation and had no significant difference for 7 and 11 days’ culture. The results of cell cycle also suggested that the membrane has no negative influence on cell division. The nHA/PA66 membranes were then implanted into subcutaneous sites of nine Sprague Dawley rats. The wounds and implant sites were free from suppuration and necrosis in all periods. All nHA/PA66 membranes were surrounded by a fibrous capsule with decreasing thickness 1 to 8 weeks postoperatively. In conclusion, the results of the in vitro and in vivo studies reveal that nHA/PA66 membrane has excellent biocompatibility and indicate its use in guided tissue regeneration (GTR or GBR.Keywords: hydroxyapatite/polyamide, barrier membrane, biocompatibility, guided bone regeneration

  8. Electrochemical polishing as a 316L stainless steel surface treatment method: Towards the improvement of biocompatibility

    International Nuclear Information System (INIS)

    Highlights: • Electropolishing of 316L stainless steel increases its corrosion resistance. • New electropolishing electrolyte composition is suggested. • Larger thickness and chromium enrichment of the passive film is obtained. • Electropolishing improves the surface biocompatibility and hemocompatibility. - Abstract: A 316L stainless steel (316L-SS) surface was electrochemically polished (EP) in an electrolyte of a new chemical composition at different cell voltages, with the aim of improving its corrosion resistance and biocompatibility. X-ray photoelectron spectroscopy results revealed that the EP-formed oxide films were characterized by a significantly higher atomic Cr/Fe ratio and film thickness, in comparison to the naturally-grown passive oxide film formed on the untreated (control) 316L-SS surface. As a result of the increase in the oxide film thickness and relative Cr enrichment, the EP-treated 316L-SS surfaces offered a notable improvement in general corrosion resistance and pitting potential. In addition, the attachment of endothelial cells (ECs) and smooth muscle cells (SMCs) to the 316L-SS surfaces revealed a positive effect of electropolishing on the preferential attachment of ECs, thus indicating that the EP surfaces could be endothelialized faster than the control (unmodified) 316L-SS surface. Furthermore, the EP surfaces showed a much lower degree of thrombogenicity in experiments with the platelet-rich plasma. Therefore, the use of the electrochemical polishing technique in treating a 316L-SS surface, under the conditions presented in this paper, indicates a significant improvement in the surface’s performance as an implant material

  9. Biocompatibility evaluation of Minalux and VeraBond2 in-vitro

    Directory of Open Access Journals (Sweden)

    Kandi Bidgoli M.

    2005-05-01

    Full Text Available Statement of Problem: One of newly presented base metal alloys (Minalux is produced according to VeraBond2 alloy (Ni- Cr base composition. Several studies showed that, cytotoxicity of base metal alloys can be occurred due to corrosion and element release. Purpose: This study evaluated the biocompatibility of these two base metal alloys in three steps: as cast, after polishing and after porcelain firing cycles. Release of Ni and Cr ions were measured to determine if there is any difference between these two alloys. Materials and Methods: Samples of two base metal alloys were subjected to Neutral Red Assay, MTT Assay and Trypan Blue for biocompatibility tests. Fibroblast Balb/c 3T3 cells were used for cell culture. Samples were contacted directly with cells in 37ºc and 5% Co2 concentration for 72 hours. Teflon samples were used as negative control. ANOVA test was used to compare different groups of two alloys. In addition, the release of Ni and Cr ions in to saline solution was measured by means of atomic absorption spectrometry. Results: MTT and Trypan Blue didn’t show any significant difference between Minalux, VeraBond2 and Teflon. Neutral Red Assay showed no significant difference between these two base metal alloys but as cast group was higher in cytotoxicity in comparisons with polished and firing groups in both two alloys. Release of Cr ion was non detectable (Cr < 1 PPB but Ni ion was measured and Ni release was significantly different in as cast groups (P=0.007 of two alloys. Conclusion: There is no significant difference between cytotoxicity of two base metal alloys and polishing and firing can decrease cytotoxicity of both alloys.

  10. Morphology characterization and biocompatibility study of PLLA (Poly-L-Llactid-Acid) coating chitosan as stent for coronary heart disease

    Science.gov (United States)

    Widiyanti, Prihartini; Paramadini, Adanti W.; Jabbar, Hajria; Fatimah, Inas; Nisak, Fadila N. K.; Puspitasari, Rahma A.

    2016-03-01

    Cardiovascular disease is a global disease with high urgency. In the severe case of coronary heart disease while a blockage in the coronary arteries reach 75% or more, the patient required stent implantation. Stents are made of metal which has many limitations that can lead to blood clots and stent incompatibility toward the size of the blood vessels. There is a metal stent replacement solution that made from polymer material which is biocompatible. PLLA also has biocompatibility and good mechanical strength. PLLA stent will be coated with chitosan as a candidate for drug-coated stents which is able to work as a drug carrier. The aim of this study is to know the morphology information and biocompability status of PLLA coating chitosan as candidate of heart stent. Morphological results using SEM showed a smooth surface structure which reinforced clinical standard of stent material. Results of cytotoxicity test by MTT Assay method showed that the result of four samples in this experiment living cells is reached 90% which is non toxic and safe to use in the human body. %). The conclusion of this study is PLLA is polymer has potency to be used as stent material.

  11. Biocompatibility of a restorative resin-modified glass ionomer cement applied in very deep cavities prepared in human teeth.

    Science.gov (United States)

    Soares, Diana Gabriela; Basso, Fernanda Gonçalves; Scheffel, Débora Lopes Sales; Giro, Elisa Maria Aparecida; de Souza Costa, Carlos Alberto; Hebling, Josimeri

    2016-01-01

    This study evaluated whether a restorative resin-modified glass ionomer cement, Vitremer (VM), would be biocompatible with pulp tissue when used as a liner in very deep cavities prepared in young human permanent teeth. Two dental cements in current use as liner materials, Vitrebond (VB) and Dycal (DY), were compared to VM. Class V cavities were prepared in 36 sound premolars that were scheduled for extraction, and the cavity floor was lined with the restorative cement (VM) or a liner/base control cement (VB or DY). For VM specimens, the cavity floor was pretreated with a primer (polyacrylic acid plus 2-hydroxyethyl methacrylate). Teeth were extracted after 7 or 30 days and processed for microscopic evaluation. In the VM group, inward diffusion of dental material components through dentinal tubules, associated with disruption of the odontoblastic layer, moderate to intense inflammatory response, and resorption of inner dentin, was observed in 2 teeth at 7 days. These histologic features were observed in 1 tooth at 30 days. In the VB group, mild inflammatory reactions and tissue disorganization observed at 7 days were resolved at 30 days. No pulpal damage occurred in the DY specimens. Of the materials tested, only Vitremer was not considered biocompatible, because it caused persistent pulpal damage when applied in very deep cavities (remaining dentin thickness less than 0.3 mm). PMID:27367631

  12. Translational Applications of Nanodiamonds: From Biocompatibility to Theranostics

    Science.gov (United States)

    Moore, Laura Kent

    Nanotechnology marks the next phase of development for drug delivery, contrast agents and gene therapy. For these novel systems to achieve success in clinical translation we must see that they are both effective and safe. Diamond nanoparticles, also known as nanodiamonds (NDs), have been gaining popularity as molecular delivery vehicles over the last decade. The uniquely faceted, carbon nanoparticles possess a number of beneficial properties that are being harnessed for applications ranging from small-molecule drug delivery to biomedical imaging and gene therapy. In addition to improving the effectiveness of a variety of therapeutics and contrast agents, initial studies indicate that NDs are biocompatible. In this work we evaluate the translational potential of NDs by demonstrating efficacy in molecular delivery and scrutinizing particle tolerance. Previous work has demonstrated that NDs are effective vehicles for the delivery of anthracycline chemotherapeutics and gadolinium(III) based contrast agents. We have sought to enhance the gains made in both areas through the addition of active targeting. We find that ND-mediated targeted delivery of epirubicin to triple negative breast cancers induces tumor regression and virtually eliminates drug toxicities. Additionally, ND-mediated delivery of the MRI contrast agent ProGlo boosts the per gadolinium relaxivity four fold, eliminates water solubility issues and effectively labels progesterone receptor expressing breast cancer cells. Both strategies open the door to the development of targeted, theranostic constructs based on NDs, capable of treating and labeling breast cancers at the same time. Although we have seen that NDs are effective vehicles for molecular delivery, for any nanoparticle to achieve clinical utility it must be biocompatible. Preliminary research has shown that NDs are non-toxic, however only a fraction of the ND-subtypes have been evaluated. Here we present an in depth analysis of the cellular

  13. Accessing the biocompatibility of layered double hydroxide by intramuscular implantation: histological and microcirculation evaluation.

    Science.gov (United States)

    Cunha, Vanessa Roberta Rodrigues; de Souza, Rodrigo Barbosa; da Fonseca Martins, Ana Maria Cristina Rebello Pinto; Koh, Ivan Hong Jun; Constantino, Vera Regina Leopoldo

    2016-01-01

    Biocompatibility of layered double hydroxides (LDHs), also known as hydrotalcite-like materials or double metal hydroxides, was investigated by in vivo assays via intramuscular tablets implantation in rat abdominal wall. The tablets were composed by chloride ions intercalated into LDH of magnesium/aluminum (Mg2Al-Cl) and zinc/aluminum (Zn2Al-Cl). The antigenicity and tissue integration capacity of LDHs were assessed histologically after 7 and 28 days post-implantation. No fibrous capsule nearby the LDH was noticed for both materials as well any sign of inflammatory reactions. Sidestream Dark Field imaging, used to monitor in real time the microcirculation in tissues, revealed overall integrity of the microcirculatory network neighboring the tablets, with no blood flow obstruction, bleeding and/or increasing of leukocyte endothelial adhesion. After 28 days Mg2Al-Cl promoted multiple collagen invaginations (mostly collagen type-I) among its fragments while Zn2Al-Cl induced predominantly collagen type-III. This work supports previous results in the literature about LDHs compatibility with living matter, endorsing them as functional materials for biomedical applications. PMID:27480483

  14. Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite.

    Science.gov (United States)

    Pelto, Jani; Haimi, Suvi; Puukilainen, Esa; Whitten, Philip G; Spinks, Geoffrey M; Bahrami-Samani, Mehrdad; Ritala, Mikko; Vuorinen, Tommi

    2010-06-01

    Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA-CNT) was studied in situ by electrochemical atomic force microscopy (EC-AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT-cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST-1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical-QCM (EC-QCM) analysis of a 210-nm thick film reveals that the material is very soft G' approximately 100 kPa and swells upon reduction. EC-AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20-25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA-CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide. PMID:19753624

  15. Temperature-Responsive Biocompatible Copolymers Incorporating Hyperbranched Polyglycerols for Adjustable Functionality

    Directory of Open Access Journals (Sweden)

    Alan J. House

    2011-08-01

    Full Text Available Temperature-triggered copolymers are proposed for a number of bio-applications but there is no ideal material platform, especially for injectable drug delivery. Options are needed for degradable biomaterials that not only respond to temperature but also easily accommodate linkage of active molecules. A first step toward realizing this goal is the design and synthesis of the novel materials reported herein. A multifunctional macromer, methacrylated hyperbranched polyglycerol (HPG-MA with an average of one acrylate unit per copolymer, was synthesized and copolymerized with N-isopropylacrylamide (NIPAAm, hydroxyethyl methacrylate-polylactide (HEMAPLA and acrylic acid (AAc. The potential to fully exploit the copolymers by modification of the multiple HPG hydroxyl groups will not be discussed here. Instead, this report focuses on the thermoresponsive, biocompatible, and degradation properties of the material. Poly(NIPAAm-co-HEMAPLA-co-AAc-co-HPG-MA displayed increasing lower critical solution temperatures (LCST as the HPG content increased over a range of macromer ratios. For the copolymer with the maximum HPG incorporation (17%, the LCST was ~30 °C. In addition, this sample showed no toxicity when human uterine fibroid cells were co-cultured with the copolymer for up to 72 h. This copolymer lost approximately 92% of its mass after 17 hours at 37 °C. Thus, the reported biomaterials offer attractive properties for the design of drug delivery systems where orthogonally triggered mechanisms of therapeutic release in relatively short time periods would be attractive.

  16. Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Wang, Zhenyu; Kutter, Jörg Peter;

    2006-01-01

    There is an ever increasing need to find surfaces that are biocompatible for applications like medical implants and microfluidics-based cell culture systems. The biocompatibility of five different surfaces with different hydrophobicity was determined using gene expression profiling as well as more...

  17. Effects of surface finishing conditions on the biocompatibility of a nickel-chromium dental casting alloy.

    LENUS (Irish Health Repository)

    McGinley, Emma Louise

    2011-07-01

    To assess the effects of surface finishing condition (polished or alumina particle air abraded) on the biocompatibility of direct and indirect exposure to a nickel-chromium (Ni-Cr) d.Sign®10 dental casting alloy on oral keratinocytes. Biocompatibility was performed by assessing cellular viability and morphology, metabolic activity, cellular toxicity and presence of inflammatory cytokine markers.

  18. The effect of cross-linking on the microstructure, mechanical properties and biocompatibility of electrospun polycaprolactone–gelatin/PLGA–gelatin/PLGA–chitosan hybrid composite

    OpenAIRE

    Thi-Hiep Nguyen; Byong-Taek Lee

    2012-01-01

    In this study, multilayered scaffolds composed of polycaprolactone (PCL)–gelatin/poly(lactic-co-glycolic acid) (PLGA)–gelatin/PLGA–chitosan artificial blood vessels were fabricated using a double-ejection electrospinning system. The mixed fibers from individual materials were observed by scanning electron microscopy. The effects of the cross-linking process on the microstructure, mechanical properties and biocompatibility of the fibers were examined. The tensile stress and liquid strength of ...

  19. Softec HD hydrophilic acrylic intraocular lens: biocompatibility and precision

    Directory of Open Access Journals (Sweden)

    Ladan Espandar

    2011-01-01

    Full Text Available Ladan Espandar1, Shameema Sikder2, Majid Moshirfar31Department of Ophthalmology, Tulane University, New Orleans, LA, USA; 2Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD, USA; 3John A Moran Eye Center, University of Utah, Salt Lake City, UT, USAAbstract: Intraocular lens development is driven by higher patient expectations for ideal visual outcomes. The recently US Food and Drug Administration-approved Softec HD™ lens is an aspheric, hydrophilic acrylic intraocular lens (IOL. The hydrophilic design of the lens is optimized to address dysphotopsia while maintaining biocompatibility, optical clarity, resistance to damage, and resistance to biocontamination. Aspheric lenses decrease postoperative spherical aberration. The addition of the Softec lens provides clinicians with another option for IOL placement; however, randomized comparative studies of this lens to others already on the market remain to be completed.Keywords: hydrophilic acrylic intraocular lens, Softec HD intraocular lens, aspheric intraocular lens, IOL

  20. Rapid microfabrication of solvent-resistant biocompatible microfluidic devices.

    Science.gov (United States)

    Hung, Lung-Hsin; Lin, Robert; Lee, Abraham Phillip

    2008-06-01

    This paper presents a rapid, simple, and low-cost fabrication method to prepare solvent resistant and biocompatible microfluidic devices with three-dimensional geometries. The devices were fabricated in thiolene and replicated from PDMS master with high molding fidelity. Good chemical compatibility for organic solvents allows volatile chemicals in synthesis and analysis applications. The surface can be processed to be hydrophobic or hydrophilic for water-in-oil and oil-in-water emulsions. Monodisperse organic solvent droplet generation is demonstrated to be reproducible in thiolene microchannels without swelling. The thiolene surface prevents cell adhesion but normal cell growth and adhesion on glass substrates is not affected by the adjacent thiolene patterns. PMID:18497921

  1. Biocompatibility of epoxidized styrene-butadiene-styrene block copolymer membrane

    International Nuclear Information System (INIS)

    Styrene-butadiene-styrene block copolymer (SBS) membrane was prepared by solution casting method and then was epoxidized with peroxyformic acid generated in situ to yield the epoxidized styrene-butadiene-styrene block copolymer membrane (ESBS). The structure and properties of ESBS were characterized with infrared spectroscopy, Universal Testing Machine, differential scanning calorimetry (DSC), and thermogravimetry analysis (TGA). The performances of contact angle, water content, protein adsorption, and water vapor transmission rate on ESBS membrane were determined. After epoxidation, the hydrophilicity of the membrane increased. The water vapor transmission rate of ESBS membrane is similar to human skin. The biocompatibility of ESBS membrane was evaluated with the cell culture of fibroblasts on the membrane. It revealed that the cells not only remained viable but also proliferated on the surface of the various ESBS membranes and the population doubling time for fibroblast culture decreased.

  2. Vertically, interconnected carbon nanowalls as biocompatible scaffolds for osteoblast cells

    Science.gov (United States)

    Ion, Raluca; Vizireanu, Sorin; Luculescu, Catalin; Cimpean, Anisoara; Dinescu, Gheorghe

    2016-07-01

    The response of MC3T3-E1 pre-osteoblasts to vertically aligned, interconnected carbon nanowalls prepared by plasma enhanced chemical vapor deposition on silicon substrate has been evaluated in terms of cell adhesion, viability and cell proliferation. The behavior of osteoblasts seeded on carbon nanowalls was analyzed in parallel and compared with the behavior of the cells maintained in contact with tissue culture polystyrene (TCPS). The results demonstrate that osteoblasts adhere and remain viable in the long term on carbon nanowalls. Moreover, on the investigated scaffold cell proliferation was significantly promoted, although to a lower extent than on TCPS. Overall, the successful culture of osteoblasts on carbon nanowalls coated substrate confirms the biocompatibility of this scaffold, which could have potential applications in the development of orthopedic biomaterials.

  3. High content analysis of the biocompatibility of nickel nanowires

    Energy Technology Data Exchange (ETDEWEB)

    Byrne, Fiona [CRANN and School of Physics, Trinity College, Dublin 2 (Ireland)], E-mail: fibyrne@tcd.ie; Prina-Mello, Adriele [CRANN and School of Physics, Trinity College, Dublin 2 (Ireland); Whelan, Aine [CRANN and School of Chemistry, Trinity College, Dublin 2 (Ireland); Mohamed, Bashir M.; Davies, Anthony [Department of Clinical Medicine, Trinity College Health Sciences Centre, Dublin 8 (Ireland); Gun' ko, Yurii K. [CRANN and School of Chemistry, Trinity College, Dublin 2 (Ireland); Coey, J.M.D. [CRANN and School of Physics, Trinity College, Dublin 2 (Ireland); Volkov, Yuri [CRANN and School of Physics, Trinity College, Dublin 2 (Ireland); Department of Clinical Medicine, Trinity College Health Sciences Centre, Dublin 8 (Ireland)

    2009-05-15

    Nickel nanowires, 20 {mu}m long and 200 nm in diameter, were fabricated by electrodeposition into alumina templates, and characterised by superconducting quantum interference device (SQUID) magnetometer, X-ray diffraction and scanning electron microscopy. Biocompatibility studies of nickel nanowires with differentiated THP-1 cell line-derived macrophages were carried out. From a multiparametric assay, using high content analysis (HCA), the critical time points and concentrations of nickel nanowires on THP-1 cellular response were identified. The nanowires displayed little or no toxic effects on THP-1 cells over short incubation times (10 h), and at low concentrations (<100 nanowires per cell). Our findings indicate the potential suitability of these wires for biological and clinical applications.

  4. Biocompatible benzocyclobutene-based intracortical neural implant with surface modification

    Science.gov (United States)

    Lee, Keekeun; Massia, Stephen; He, Jiping

    2005-11-01

    This paper presents the fabrication of a benzocyclobutene (BCB) polymer-based intracortical neural implant for reliable and stable long-term implant function. BCB polymer has many attractive features for chronic implant application: flexibility, biocompatibility, low moisture uptake, low dielectric constant and easy surface modification. A 2 µm thick silicon backbone layer was attached underneath a flexible BCB electrode to improve mechanical stiffness. No insertion trauma was observed during penetrating into the dura of a rat. In vitro cytotoxicity tests of the completed BCB electrode revealed no toxic effects on cultured cells. The modified BCB surface with a dextran coating showed a significant reduction in 3T3 cell adhesion and spreading, indicating that this coating has the potential for lowering protein adsorption, minimizing inflammatory cell adhesion and glial scar formation in vivo, and thereby enhancing long-term implant performance.

  5. Preparation and biocompatibility of BSA monolayer on silicon surface.

    Science.gov (United States)

    Tao, Caihong; Zhang, Junyan; Yang, Shengrong

    2011-06-01

    This paper describes a general strategy for grafting protein molecules on silicon surface by using dopamine as adhesive layer. With this method, silicon surface had been successfully modified by BSA monolayer. Fourier transform infrared spectra, X-ray photoelectron spectroscopy, contact angle analysis and atomic force microscopy confirmed the sequential grafting of initiator and protein molecules. Cell adhesion experiments with PC-12 cells showed that the obtained monolayer exhibits good biocompatibility. The corrosion resistance behavior of the polydopamine and BSA modified silicon wafers was investigated by potentiodynamic test, which indicated that the modified surfaces exhibited a better anti-corrosion capability than silicon surface. All these results must be valuable for the application of protein monolayer in biological and biomedical technology. PMID:21770145

  6. How hydrophobically modified chitosans are stabilized by biocompatible lipid aggregates.

    Science.gov (United States)

    Ruocco, Nino; Frielinghaus, Heide; Vitiello, Giuseppe; D'Errico, Gerardino; Leal, Leslie G; Richter, Dieter; Ortona, Ornella; Paduano, Luigi

    2015-08-15

    Nanostructured hydrogels composed by biocompatible molecules are formulated and characterized. They are based on a polymer network formed by hydrophobically modified chitosans (HMCHIT or CnCHIT) in which vesicles of monoolein (MO) and oleic acid or sodium oleate (NaO), depending on pH, are embedded. The best conditions for gel formation, in terms of pH, length of the hydrophobic moieties of chitosan, and weight proportion among the three components were estimated by visual inspection of a large number of samples. Among all possible combinations, the system C12CHIT-MO-NaO in the weight proportion (1:1:1) is optimal for the formation of a well-structured gel-like system, which is also confirmed by rheological experiments. Electron paramagnetic resonance (EPR) measurements unambiguously show the presence of lipid bilayers in this mixture, indicating that MO-NaO vesicles are stabilized by C12CHIT even at acid pH. A wide small angle neutron scattering investigation performed on several ternary systems of general formula CnCHIT-MO-NaO shows that the length of the hydrophobic tail Cn is a crucial parameter in stabilizing the polymer network in which lipid vesicles are embedded. Structural parameters for the vesicles are determined by using a multilamellar model that admits the possibility of displacement of the center of each shell. The number of shells tends to be reduced by increasing the polymer content. The thickness and the distance between consecutive lamellae are not influenced by either the polymer or MO-NaO concentration. The hydrogel presented in this work, being fully biocompatible and nanostructured, is well-suited for possible application in drug delivery. PMID:25935287

  7. Biocompatibility of chitosan/Mimosa tenuiflora scaffolds for tissue engineering

    International Nuclear Information System (INIS)

    Highlights: • The porosity of the composites allow biological processes for the cell adaptation on the scaffolds. • The composites improve the viability and proliferation of cells. • Composition of the scaffold plays an important role in the biocompatibility. • The results indicate that Mimosa Tenuiflora can induce the differentiation of osteoblast cells. - Abstract: In search of a plant that exhibits osteogenic activity, Mimosa tenuiflora (M. tenuiflora) cortex represents the opportunity to create a biomaterial that, together with the chitosan, is osteoconductive and promote better and rapid regeneration of bone tissue. Thus, the composite of chitosan/M. tenuiflora cortex fabricated will have properties of biocompatibility and allow the osteoblast proliferation. Composites were developed with different concentrations of chitosan/M. tenuiflora cortex (w/w) using thermally induced phase separation technique (TIPS). To analyze the effects of composite on osteoblasts, primary cultures, each sample was collected on days 1, 3 and 7 after seeding. The evaluation of composites consisted of viability and proliferation tests in which we observed the metabolic activity of the cells using MTT reagent and determined the DNA concentration by means of fluorescence. The expression of the marker alkaline phosphatase (ALP) using p-nitrophenyl phosphate was examined, allowing the observation to the activity of proliferation and differentiation of osteoblastic cells. Moreover, an analysis of biomineralization was performed using scanning electron microscopy (SEM), energy dispersive spectroscopy, infrared spectroscopy and X-ray diffraction. The results showed that 80/20 chitosan/M. tenuiflora cortex biocomposite has the best performance with osteoblasts compared to biomaterials 100/0 and 70/30 chitosan/M. tenuiflora composites. Finally, it was determined that the composite of chitosan/M. tenuiflora cortex presents no cytotoxicity and increases the capacity of the osteoblasts

  8. Biocompatibility of chitosan/Mimosa tenuiflora scaffolds for tissue engineering

    Energy Technology Data Exchange (ETDEWEB)

    Martel-Estrada, Santos Adriana [Instituto de arquitectura diseño y arte, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); Rodríguez-Espinoza, Brenda [Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); Santos-Rodríguez, Elí [ICTP Meso-American Centre for Theoretical Physics (ICTP-MCTP)/Universidad Autónoma de Chiapas, Ciudad Universitaria, Carretera Zapata Km. 4, Real del Bosque (Terán), C.P. 29040 Tuxtla Gutiérrez, Chiapas (Mexico); Jiménez-Vega, Florinda [Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); García-Casillas, Perla E.; Martínez-Pérez, Carlos A. [Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P. 32320 Cd. Juárez, Chihuahua (Mexico); and others

    2015-09-15

    Highlights: • The porosity of the composites allow biological processes for the cell adaptation on the scaffolds. • The composites improve the viability and proliferation of cells. • Composition of the scaffold plays an important role in the biocompatibility. • The results indicate that Mimosa Tenuiflora can induce the differentiation of osteoblast cells. - Abstract: In search of a plant that exhibits osteogenic activity, Mimosa tenuiflora (M. tenuiflora) cortex represents the opportunity to create a biomaterial that, together with the chitosan, is osteoconductive and promote better and rapid regeneration of bone tissue. Thus, the composite of chitosan/M. tenuiflora cortex fabricated will have properties of biocompatibility and allow the osteoblast proliferation. Composites were developed with different concentrations of chitosan/M. tenuiflora cortex (w/w) using thermally induced phase separation technique (TIPS). To analyze the effects of composite on osteoblasts, primary cultures, each sample was collected on days 1, 3 and 7 after seeding. The evaluation of composites consisted of viability and proliferation tests in which we observed the metabolic activity of the cells using MTT reagent and determined the DNA concentration by means of fluorescence. The expression of the marker alkaline phosphatase (ALP) using p-nitrophenyl phosphate was examined, allowing the observation to the activity of proliferation and differentiation of osteoblastic cells. Moreover, an analysis of biomineralization was performed using scanning electron microscopy (SEM), energy dispersive spectroscopy, infrared spectroscopy and X-ray diffraction. The results showed that 80/20 chitosan/M. tenuiflora cortex biocomposite has the best performance with osteoblasts compared to biomaterials 100/0 and 70/30 chitosan/M. tenuiflora composites. Finally, it was determined that the composite of chitosan/M. tenuiflora cortex presents no cytotoxicity and increases the capacity of the osteoblasts

  9. Nanocrystalline β-Ti alloy with high hardness, low Young's modulus and excellent in vitro biocompatibility for biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Xie, Kelvin Y. [Australian Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, NSW 2006 (Australia); School of Aerospace, Mechanical and Mechatronics Engineering, The University of Sydney, Sydney, NSW 2006 (Australia); Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218 (United States); Wang, Yanbo, E-mail: yanbo.wang@sydney.edu.au [School of Aerospace, Mechanical and Mechatronics Engineering, The University of Sydney, Sydney, NSW 2006 (Australia); Zhao, Yonghao [School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094 (China); Chang, Li; Wang, Guocheng; Chen, Zibin; Cao, Yang [School of Aerospace, Mechanical and Mechatronics Engineering, The University of Sydney, Sydney, NSW 2006 (Australia); Liao, Xiaozhou, E-mail: xiaozhou.liao@sydney.edu.au [School of Aerospace, Mechanical and Mechatronics Engineering, The University of Sydney, Sydney, NSW 2006 (Australia); Lavernia, Enrique J. [Department of Chemical Engineering and Materials Science, University of California, Davis, CA 95616 (United States); Valiev, Ruslan Z. [Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, K. Marksa 12, Ufa 450000 (Russian Federation); Sarrafpour, Babak; Zoellner, Hans [The Cellular and Molecular Pathology Research Unit, Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, The University of Sydney, Westmead Centre for Oral Health, Westmead Hospital, NSW 2145 (Australia); Ringer, Simon P. [Australian Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, NSW 2006 (Australia); School of Aerospace, Mechanical and Mechatronics Engineering, The University of Sydney, Sydney, NSW 2006 (Australia)

    2013-08-01

    High strength, low Young's modulus and good biocompatibility are desirable but difficult to simultaneously achieve in metallic implant materials for load bearing applications, and these impose significant challenges in material design. Here we report that a nano-grained β-Ti alloy prepared by high-pressure torsion exhibits remarkable mechanical and biological properties. The hardness and modulus of the nano-grained Ti alloy were respectively 23% higher and 34% lower than those of its coarse-grained counterpart. Fibroblast cell attachment and proliferation were enhanced, demonstrating good in vitro biocompatibility of the nano-grained Ti alloy, consistent with demonstrated increased nano-roughness on the nano-grained Ti alloy. Results suggest that the nano-grained β-Ti alloy may have significant application as an implant material in dental and orthopedic applications. - Highlights: • A bulk nanocrystalline β-Ti alloy was produced by high-pressure torsion processing. • Excellent mechanical properties for biomedical implants were obtained. • Enhanced in vitro biocompatibility was also demonstrated.

  10. Crystalline Bioceramic Materials

    OpenAIRE

    Aza Moya, Antonio H. de; Aza, Piedad N. de; Aza Pendas, Salvador de

    2005-01-01

    [EN] A strong interest in the use of ceramics for biomedical engineering applications developed in the late 1960´s. Used initially as alternatives to metallic materials in order to increase the biocompatibility of implants, bioceramics have become a diverse class of biomaterials, presently including three basic types: relatively bioinert ceramics; bioactive or surface reactive bioceramics and bioresorbable ceramics. This review will only refer to bioceramics “sensus stricto”, it i...

  11. In vitro degradation behavior and biocompatibility of Mg–Nd–Zn–Zr alloy by hydrofluoric acid treatment

    International Nuclear Information System (INIS)

    In this paper, Mg–Nd–Zn–Zr alloy (denoted as JDBM) coated with hydrofluoric acid (HF) chemical conversion film (MgF2) was researched as a potential biodegradable cardiovascular stent material. The microstructures, in vitro degradation and biocompatibility were investigated. The field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS) showed that a compact MgF2 film was formed on the surface of JDBM. The corrosion rate decreased in artificial plasma from 0.337 to 0.253 mm·y−1 and the electrochemical measurement demonstrated that the corrosion resistance of JDBM alloy could be obviously improved due to the protective MgF2 film on the surface of the substrate. Meanwhile, the hemolysis ratio of JDBM decreased from 52.0% to 10.1% and the cytotoxicity met the requirement of cellular application after HF treatment. In addition, JDBM and MgF2 film showed good anti-platelet adhesion, which is a very favorable property for implant material in contact with blood directly. - Highlights: ► We have prepared a uniform and dense MgF2 film on JDBM alloy. ► The corrosion rate of JDBM can be decreased by HF treatment. ► The biocompatibility of JDBM can be improved by HF treatment. ► JDBM showed uniform corrosion in artificial plasma.

  12. Nanocrystalline Cellulose Improves the Biocompatibility and Reduces the Wear Debris of Ultrahigh Molecular Weight Polyethylene via Weak Binding.

    Science.gov (United States)

    Wang, Shiwen; Feng, Qiang; Sun, Jiashu; Gao, Feng; Fan, Wei; Zhang, Zhong; Li, Xiaohong; Jiang, Xingyu

    2016-01-26

    The doping of biocompatible nanomaterials into ultrahigh molecular weight polyethylene (UHMWPE) to improve the biocompatibility and reduce the wear debris is of great significance to prolonging implantation time of UHMWPE as the bearing material for artificial joints. This study shows that UHMWPE can form a composite with nanocrystalline cellulose (NCC, a hydrophilic nanosized material with a high aspect ratio) by ball-milling and hot-pressing. Compared to pure UHMWPE, the NCC/UHMWPE composite exhibits improved tribological characteristics with reduced generation of wear debris. The underlying mechanism is related to the weak binding between hydrophilic NCC and hydrophobic UHMWPE. The hydrophilic, rigid NCC particles tend to detach from the UHMWPE surface during friction, which could move with the rubbing surface, serve as a thin lubricant layer, and protect the UHMWPE substrate from abrasion. The biological safety of the NCC/UHMWPE composite, as tested by MC3T3-E1 preosteoblast cells and macrophage RAW264.7 cells, is high, with significantly lower inflammatory responses/cytotoxicity than pure UHMWPE. The NCC/UHMWPE composite therefore could be a promising alternative to the current UHMWPE for bearing applications. PMID:26687771

  13. A Comparison of Biocompatibility of a Titanium Alloy Fabricated by Electron Beam Melting and Selective Laser Melting

    Science.gov (United States)

    Wang, Hong; Zhao, Bingjing; Liu, Changkui; Wang, Chao; Tan, Xinying; Hu, Min

    2016-01-01

    Electron beam melting (EBM) and selective laser melting (SLM) are two advanced rapid prototyping manufacturing technologies capable of fabricating complex structures and geometric shapes from metallic materials using computer tomography (CT) and Computer-aided Design (CAD) data. Compared to traditional technologies used for metallic products, EBM and SLM alter the mechanical, physical and chemical properties, which are closely related to the biocompatibility of metallic products. In this study, we evaluate and compare the biocompatibility, including cytocompatibility, haemocompatibility, skin irritation and skin sensitivity of Ti6Al4V fabricated by EBM and SLM. The results were analysed using one-way ANOVA and Tukey’s multiple comparison test. Both the EBM and SLM Ti6Al4V exhibited good cytobiocompatibility. The haemolytic ratios of the SLM and EBM were 2.24% and 2.46%, respectively, which demonstrated good haemocompatibility. The EBM and SLM Ti6Al4V samples showed no dermal irritation when exposed to rabbits. In a delayed hypersensitivity test, no skin allergic reaction from the EBM or the SLM Ti6Al4V was observed in guinea pigs. Based on these results, Ti6Al4V fabricated by EBM and SLM were good cytobiocompatible, haemocompatible, non-irritant and non-sensitizing materials. Although the data for cell adhesion, proliferation, ALP activity and the haemolytic ratio was higher for the SLM group, there were no significant differences between the different manufacturing methods. PMID:27391895

  14. Microstructure, mechanical properties, and in vitro biocompatibility of spark plasma sintered hydroxyapatite-aluminum oxide-carbon nanotube composite

    International Nuclear Information System (INIS)

    In the present work, HA reinforced with Al2O3 and multiwalled carbon nanotubes (CNTs) is processed using spark plasma sintering (SPS). Vickers micro indentation and nanoindentation of the samples revealed contrary mechanical properties (hardness of 4.0, 6.1, and 4.4 GPa of HA, HA-Al2O3 and HA-Al2O3-CNT samples at bulk scale, while that of 8.0, 9.0, and 7.0 GPa respectively at nanoscale), owing to the difference in the interaction of the indenter with the material at two different length scales. The addition of Al2O3 reinforcement has been shown to enhance the indentation fracture toughness of HA matrix from 1.18 MPa m1/2 to 2.07 MPa m1/2. Further CNT reinforcement has increased the fracture toughness to 2.3 times (2.72 MPa m1/2). In vitro biocompatibility of CNT reinforced HA-Al2O3 composite has been evaluated using MTT assay on mouse fibroblast L929 cell line. Cell adhesion and proliferation have been characterized using scanning electron microscopy (SEM), and have been quantified using UV spectrophotometer. The combination of cell viability data as well as microscopic observations of cultured surfaces suggests that SPS sintered HA-Al2O3-CNT composites exhibit the ability to promote cell adhesion and proliferation on their surface and prove to be promising new biocompatible materials.

  15. Osseointegration and biocompatibility of different metal implants - a comparative experimental investigation in sheep

    Directory of Open Access Journals (Sweden)

    Plecko Michael

    2012-03-01

    Full Text Available Abstract Background In the present study, 4 different metallic implant materials, either partly coated or polished, were tested for their osseointegration and biocompatibility in a pelvic implantation model in sheep. Methods Materials to be evaluated were: Cobalt-Chrome (CC, Cobalt-Chrome/Titanium coating (CCTC, Cobalt-Chrome/Zirconium/Titanium coating (CCZTC, Pure Titanium Standard (PTST, Steel, TAN Standard (TANST and TAN new finish (TANNEW. Surgery was performed on 7 sheep, with 18 implants per sheep, for a total of 63 implants. After 8 weeks, the specimens were harvested and evaluated macroscopically, radiologically, biomechanically (removal torque, histomorphometrically and histologically. Results Cobalt-Chrome screws showed significantly (p = 0.031 lower removal torque values than pure titanium screws and also a tendency towards lower values compared to the other materials, except for steel. Steel screws showed no significant differences, in comparison to cobalt-chrome and TANST, however also a trend towards lower torque values than the remaining materials. The results of the fluorescence sections agreed with those of the biomechanical test. Histomorphometrically, there were no significant differences of bone area between the groups. The BIC (bone-to-implant-contact, used for the assessment of the osseointegration, was significantly lower for cobalt-chrome, compared to steel (p = 0.001. Steel again showed a lower ratio (p = 0.0001 compared to the other materials. Conclusion This study demonstrated that cobalt-chrome and steel show less osseointegration than the other metals and metal-alloys. However, osseointegration of cobalt-chrome was improved by zirconium and/or titanium based coatings (CCTC, TANST, TAN, TANNEW being similar as pure titanium in their osseointegrative behavior.

  16. Biocompatibility evaluation of sputtered zirconium-based thin film metallic glass-coated steels

    Directory of Open Access Journals (Sweden)

    Subramanian B

    2015-10-01

    Full Text Available Balasubramanian Subramanian,1 Sundaram Maruthamuthu,2 Senthilperumal Thanka Rajan1 1Electrochemical Material Science Division, 2Corrosion and Materials Protection Division, Central Electrochemical Research Institute, Karaikudi, India Abstract: Thin film metallic glasses comprised of Zr48Cu36Al8Ag8 (at.% of approximately 1.5 µm and 3 µm in thickness were prepared using magnetron sputtering onto medical grade 316L stainless steel. Their structural and mechanical properties, in vitro corrosion, and antimicrobial activity were analyzed. The amorphous thin film metallic glasses consisted of a single glassy phase, with an absence of any detectable peaks corresponding to crystalline phases. Elemental composition close to the target alloy was noted from EDAX analysis of the thin film. The surface morphology of the film showed a smooth surface on scanning electron microscopy and atomic force microscopy. In vitro electrochemical corrosion studies indicated that the zirconium-based metallic glass could withstand body fluid, showing superior resistance to corrosion and electrochemical stability. Interactions between the coated surface and bacteria were investigated by agar diffusion, solution suspension, and wet interfacial contact methods. The results indicated a clear zone of inhibition against the growth of microorganisms such as Escherichia coli and Staphylococcus aureus, confirming the antimicrobial activity of the thin film metallic glasses. Cytotoxicity studies using L929 fibroblast cells showed these coatings to be noncytotoxic in nature. Keywords: thin film metallic glasses, sputtering, biocompatibility, corrosion, antimicrobial activity

  17. Silica–polyethylene glycol hybrids synthesized by sol–gel: Biocompatibility improvement of titanium implants by coating

    Energy Technology Data Exchange (ETDEWEB)

    Catauro, M., E-mail: michelina.catauro@unina2.it [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 21, 81031 Aversa (Italy); Bollino, F.; Papale, F. [Department of Industrial and Information Engineering, Second University of Naples, Via Roma 21, 81031 Aversa (Italy); Ferrara, C.; Mustarelli, P. [Department of Chemistry, University of Pavia and INSTM, Via Taramelli 12, 27100 Pavia (Italy)

    2015-10-01

    Although metallic implants are the most used in dental and orthopaedic fields, they can early fail due to low tissue tolerance or osseointegration ability. To overcome this drawback, functional coatings can be applied on the metallic surface to provide a firm fixation of the implants. The objective of the present study was twofold: to synthesize and to characterize silica/polyethylene glycol (PEG) hybrid materials using sol–gel technique and to investigate their capability to dip-coat titanium grade 4 (Ti-gr4) substrates to improve their biological properties. Various hybrid systems have been synthesized by changing the ratio between the organic and inorganic phases in order to study the influence of the polymer amount on the structure and, thus, on the properties of the coatings. Fourier transform infrared (FTIR) spectroscopy and solid state Nuclear Magnetic Resonance (NMR) allowed us to detect the formation of hydrogen bonds between the inorganic sol–gel matrix and the organic component. SEM analysis showed that high PEG content enables to obtain crack free-coating. Moreover, the effective improvement in biological properties of Ti-gr4 implants has been evaluated by performing in vitro tests. The bioactivity of the hybrid coatings has been showed by the hydroxyapatite formation on the surface of SiO{sub 2}/PEG coated Ti-gr4 substrates after soaking in a simulated body fluid and the lack of cytotoxicity by the WST-8 Assay. The results showed that the coated substrates are more bioactive and biocompatible than the uncoated ones and that the bioactivity is not significantly affected by PEG amount whereas its addition makes the films more biocompatible. - Highlights: • SiO{sub 2}/PEG hybrid biomaterials synthesized by sol–gel method at various PEG percentages • Hybrid coating of titanium substrate with dip-coating technology • Chemical and morphological characterization of hybrids and coating • Biocompatibility improvement of coated titanium with high

  18. Potential use of porous titanium-niobium alloy in orthopedic implants: preparation and experimental study of its biocompatibility in vitro.

    Directory of Open Access Journals (Sweden)

    Jian Xu

    Full Text Available BACKGROUND: The improvement of bone ingrowth into prosthesis and enhancement of the combination of the range between the bone and prosthesis are important for long-term stability of artificial joints. They are the focus of research on uncemented artificial joints. Porous materials can be of potential use to solve these problems. OBJECTIVES/PURPOSES: This research aims to observe the characteristics of the new porous Ti-25Nb alloy and its biocompatibility in vitro, and to provide basic experimental evidence for the development of new porous prostheses or bone implants for bone tissue regeneration. METHODS: The Ti-25Nb alloys with different porosities were fabricated using powder metallurgy. The alloys were then evaluated based on several characteristics, such as mechanical properties, purity, pore size, and porosity. To evaluate biocompatibility, the specimens were subjected to methylthiazol tetrazolium (MTT colorimetric assay, cell adhesion and proliferation assay using acridine staining, scanning electron microscopy, and detection of inflammation factor interleukin-6 (IL-6. RESULTS: The porous Ti-25Nb alloy with interconnected pores had a pore size of 200 µm to 500 µm, which was favorable for bone ingrowth. The compressive strength of the alloy was similar to that of cortical bone, while with the elastic modulus closer to cancellous bone. MTT assay showed that the alloy had no adverse reaction to rabbit bone marrow mesenchymal stem cells, with a toxicity level of 0 to 1. Cell adhesion and proliferation experiments showed excellent cell growth on the surface and inside the pores of the alloy. According to the IL-6 levels, the alloy did not cause any obvious inflammatory response. CONCLUSION: All porous Ti-25Nb alloys showed good biocompatibility regardless of the percentage of porosity. The basic requirement of clinical orthopedic implants was satisfied, which made the alloy a good prospect for biomedical application. The alloy with 70

  19. Silica–polyethylene glycol hybrids synthesized by sol–gel: Biocompatibility improvement of titanium implants by coating

    International Nuclear Information System (INIS)

    Although metallic implants are the most used in dental and orthopaedic fields, they can early fail due to low tissue tolerance or osseointegration ability. To overcome this drawback, functional coatings can be applied on the metallic surface to provide a firm fixation of the implants. The objective of the present study was twofold: to synthesize and to characterize silica/polyethylene glycol (PEG) hybrid materials using sol–gel technique and to investigate their capability to dip-coat titanium grade 4 (Ti-gr4) substrates to improve their biological properties. Various hybrid systems have been synthesized by changing the ratio between the organic and inorganic phases in order to study the influence of the polymer amount on the structure and, thus, on the properties of the coatings. Fourier transform infrared (FTIR) spectroscopy and solid state Nuclear Magnetic Resonance (NMR) allowed us to detect the formation of hydrogen bonds between the inorganic sol–gel matrix and the organic component. SEM analysis showed that high PEG content enables to obtain crack free-coating. Moreover, the effective improvement in biological properties of Ti-gr4 implants has been evaluated by performing in vitro tests. The bioactivity of the hybrid coatings has been showed by the hydroxyapatite formation on the surface of SiO2/PEG coated Ti-gr4 substrates after soaking in a simulated body fluid and the lack of cytotoxicity by the WST-8 Assay. The results showed that the coated substrates are more bioactive and biocompatible than the uncoated ones and that the bioactivity is not significantly affected by PEG amount whereas its addition makes the films more biocompatible. - Highlights: • SiO2/PEG hybrid biomaterials synthesized by sol–gel method at various PEG percentages • Hybrid coating of titanium substrate with dip-coating technology • Chemical and morphological characterization of hybrids and coating • Biocompatibility improvement of coated titanium with high PEG

  20. Improvements in the corrosion resistance and biocompatibility of biomedical Ti–6Al–7Nb alloy using an electrochemical anodization treatment

    International Nuclear Information System (INIS)

    The biocompatibility of an implant material is determined by its surface characteristics. This study investigated the application of an electrochemical anodization surface treatment to improve both the corrosion resistance and biocompatibility of Ti–6Al–7Nb alloy for implant applications. The electrochemical anodization treatment produced an Al-free oxide layer with nanoscale porosity on the Ti–6Al–7Nb alloy surface. The surface topography and microstructure of Ti–6Al–7Nb alloy were analyzed. The corrosion resistance was investigated using potentiodynamic polarization curve measurements in simulated blood plasma (SBP). The adhesion and proliferation of human bone marrow mesenchymal stem cells to test specimens were evaluated using various biological analysis techniques. The results showed that the presence of a nanoporous oxide layer on the anodized Ti–6Al–7Nb alloy increased the corrosion resistance (i.e., increased the corrosion potential and decreased both the corrosion rate and the passive current) in SBP compared with the untreated Ti–6Al–7Nb alloy. Changes in the nanotopography also improved the cell adhesion and proliferation on the anodized Ti–6Al–7Nb alloy. We conclude that a fast and simple electrochemical anodization surface treatment improves the corrosion resistance and biocompatibility of Ti–6Al–7Nb alloy for biomedical implant applications. - Highlights: ► Simple/fast electrochemical anodization was applied to biomedical Ti–6Al–7Nb surface. ► Anodized surface had nano-porous topography and contained Al-free oxide layer. ► Anodized surface raised corrosion resistance in three simulated biological solutions. ► Anodized surface enhanced cell adhesion and cell proliferation. ► Electrochemical anodization has potential as biomedical implant surface treatment

  1. 内固定与外固定材料修复骨盆骨折损伤:合理选择与生物相容性%Internal and external fixation materials for the repair of pelvic fracture:reasonable choice and biocompatibility

    Institute of Scientific and Technical Information of China (English)

    郎毅; 融恺; 陈平波

    2016-01-01

    BACKGROUND:Pelvic fractures are mostly caused by high energy trauma. With the development of imaging techniques and in-depth study of the anatomical structure of the pelvis and biomechanics, internal fixation and external fixation materials are gradual y being used in the repair of pelvic fracture. OBJECTIVE:To summarize features and applications of external fixation stent material, percutaneous screw fixation, percutaneous sacral iliac screw material for internal fixation and intramedul ary tensile screw material for internal fixation after pelvic fracture. METHODS:We retrieved Wanfang Database and PubMed for studies on the application of internal fixation material and external fixation material in pelvic fracture from 1994 to 2015. Al data were analyzed and summarized. RESULTS AND CONCLUSION:Application of pelvic external fixation materials contributed to the stability of early pelvic fractures, showed smal injury, could increase the reliability of fixation. However, the biomechanical stability of external fixation materials was lower than other internal fixation, could only be used for the early temporary fixation of unstable pelvic fractures in particular cases. Internal fixation materials can achieve anatomical reduction, accorded with the requirements of the physical mechanics of the pelvis, improve the stability of the pelvis, and have become the first choice for repair of unstable pelvic fractures. Currently used methods are percutaneous hol ow screw fixation, percutaneous fixation of the sacral iliac screw, and intramedul ary lag screw fixation. The combination of external fixation and internal fixation can effectively restore the stability of the pelvic cavity. Therefore, we should consider the location, type and stability of the fracture to select the appropriate internal fixation and external fixation materials.%背景:骨盆骨折多为高能量损伤所致,随着影像学技术的发展及对骨盆解剖结构和生物力学的深入研究,内

  2. In Vitro Biocompatibility of Si Alloyed Multi-Principal Element Carbide Coatings.

    Science.gov (United States)

    Vladescu, Alina; Titorencu, Irina; Dekhtyar, Yuri; Jinga, Victor; Pruna, Vasile; Balaceanu, Mihai; Dinu, Mihaela; Pana, Iulian; Vendina, Viktorija; Braic, Mariana

    2016-01-01

    In the current study, we have examined the possibility to improve the biocompatibility of the (TiZrNbTaHf)C through replacement of either Ti or Ta by Si. The coatings were deposited on Si and 316L stainless steel substrates by magnetron sputtering in an Ar+CH4 mixed atmosphere and were examined for elemental composition, chemical bonds, surface topography, surface electrical charge and biocompatible characteristics. The net surface charge was evaluated at nano and macroscopic scale by measuring the electrical potential and work function, respectively. The biocompatible tests comprised determination of cell viability and cell attachment to the coated surface. The deposited coatings had C/(metal+Si) ratios close to unity, while a mixture of metallic carbide, free-carbon and oxidized species formed on the film surface. The coatings' surfaces were smooth and no influence of surface roughness on electrical charge or biocompatibility was found. The biocompatible characteristics correlated well with the electrical potential/work function, suggesting a significant role of surface charge in improving biocompatibility, particularly cell attachment to coating's surface. Replacement of either Ti or Ta by Si in the (TiZrNbTaHf)C coating led to an enhanced surface electrical charge, as well as to superior biocompatible properties, with best results for the (TiZrNbSiHf)C coating. PMID:27571361

  3. Renewable smart materials

    Science.gov (United States)

    Kim, Hyun Chan; Mun, Seongcheol; Ko, Hyun-U.; Zhai, Lindong; Kafy, Abdullahil; Kim, Jaehwan

    2016-07-01

    The use of renewable materials is essential in future technologies to harmonize with our living environment. Renewable materials can maintain our resources from the environment so as to overcome degradation of natural environmental services and diminished productivity. This paper reviews recent advancement of renewable materials for smart material applications, including wood, cellulose, chitin, lignin, and their sensors, actuators and energy storage applications. To further improve functionality of renewable materials, hybrid composites of inorganic functional materials are introduced by incorporating carbon nanotubes, titanium dioxide and tin oxide conducting polymers and ionic liquids. Since renewable materials have many advantages of biocompatible, sustainable, biodegradable, high mechanical strength and versatile modification behaviors, more research efforts need to be focused on the development of renewable smart materials.

  4. Determinación por Visión Artificial del Factor de Degradación en Aleaciones Biocompatibles Computer Vision for Determination of Degradation Factor in Biocompatible Alloys

    Directory of Open Access Journals (Sweden)

    Willian Aperador-Chaparro

    2013-01-01

    Full Text Available Se determinó por visión artificial el factor de degradación de una aleación biocompatible, AISI 316LVM. Para ello, se utilizó una solución fisiológica simulada (solución de Hanks, electrolito que simula la composición presente en el organismo, es decir, el ambiente donde el implante se utilizará. El comportamiento electroquímico fue evaluado mediante curvas potencio-dinámicas. La caracterización superficial se desarrolló mediante un estereoscopio y los productos de corrosión se evaluaron mediante difracción de rayos X. El sistema usó una imagen microscópica de la superficie del material en su estado natural (brillo espejo como parámetro base para la comparación, para definir en qué estado se encuentran las muestras una vez han pasado por las pruebas realizadas. Se encontró, que es posible estimar el factor de degradación o de deterioro en un material mediante un análisis topográfico del mismo.The degradation factor of a biocompatible alloy, AISI 316LVM, was determined by computer vision. For this, a simulated physiological solution (Hanks' solution which simulates the electrolyte composition in the body, that is the environment in which the implant is used. The electrochemical behavior was evaluated by potentio-dynamic curves. The surface characterization was performed using a stereoscope and corrosion products were evaluated by X-ray diffraction. The system used a microscopic image of the surface of the material in its natural state (mirror finish as a basis for comparison parameter to define what state are once samples have undergone testing. It was found that it is possible to estimate the factor of degradation or deterioration of a material through a topographic analysis.

  5. Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application.

    Science.gov (United States)

    Hussein, Kamal Hany; Park, Kyung-Mee; Kang, Kyung-Sun; Woo, Heung-Myong

    2016-10-01

    Biomaterials based on seeding of cells on decellularized scaffolds have gained increasing interest in the last few years and suggested to serve as an alternative approach to bioengineer artificial organs and tissues for transplantation. The reaction of the host toward the decellularized scaffold and transplanted cells depends on the biocompatibility of the construct. Before proceeding to the clinical application step of decellularized scaffolds, it is greatly important to apply a number of biocompatibility tests in vitro and in vivo. This review describes the different methodology involved in cytotoxicity, pathogenicity, immunogenicity and biodegradability testing for evaluating the biocompatibility of various decellularized matrices obtained from human or animals. PMID:27287176

  6. A New Biocompatible and Antibacterial Phosphate Free Glass-Ceramic for Medical Applications

    Science.gov (United States)

    Cabal, Belén; Alou, Luís; Cafini, Fabio; Couceiro, Ramiro; Sevillano, David; Esteban-Tejeda, Leticia; Guitián, Francisco; Torrecillas, Ramón; Moya, José S.

    2014-06-01

    In the attempt to find valid alternatives to classic antibiotics and in view of current limitations in the efficacy of antimicrobial-coated or loaded biomaterials, this work is focused on the development of a new glass-ceramic with antibacterial performance together with safe biocompatibility. This bactericidal glass-ceramic composed of combeite and nepheline crystals in a residual glassy matrix has been obtained using an antimicrobial soda-lime glass as a precursor. Its inhibitory effects on bacterial growth and biofilm formation were proved against five biofilm-producing reference strains. The biocompatibility tests by using mesenchymal stem cells derived from human bone indicate an excellent biocompatibility.

  7. Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells

    Directory of Open Access Journals (Sweden)

    Chen DZ

    2012-09-01

    Full Text Available Daozhen Chen,1,3,* Qiusha Tang,2,* Xiangdong Li,3,* Xiaojin Zhou,1 Jia Zang,1 Wen-qun Xue,1 Jing-ying Xiang,1 Cai-qin Guo11Central Laboratory, Wuxi Hospital for Matemaland Child Health Care Affiliated Medical School of Nanjing, Jiangsu Province; 2Department of Pathology and Pathophysiology, Medical College, Southeast University, Jiangsu Province; 3The People’s Hospital of Aheqi County, Xinjiang, China *These authors contributed equally to this workBackground: The objective of this study was to evaluate the synthesis and biocompatibility of Fe3O4 nanoparticles and investigate their therapeutic effects when combined with magnetic fluid hyperthermia on cultured MCF-7 cancer cells.Methods: Magnetic Fe3O4 nanoparticles were prepared using a coprecipitation method. The appearance, structure, phase composition, functional groups, surface charge, magnetic susceptibility, and release in vitro were characterized by transmission electron microscopy, x-ray diffraction, scanning electron microscopy-energy dispersive x-ray spectroscopy, and a vibrating sample magnetometer. Blood toxicity, in vitro toxicity, and genotoxicity were investigated. Therapeutic effects were evaluated by MTT [3-(4, 5-dimethyl-2-thiazolyl-2, 5-diphenyl-2H-tetrazolium bromide] and flow cytometry assays.Results: Transmission electron microscopy revealed that the shapes of the Fe3O4 nanoparticles were approximately spherical, with diameters of about 26.1 ± 5.2 nm. Only the spinel phase was indicated in a comparison of the x-ray diffraction data with Joint Corporation of Powder Diffraction Standards (JCPDS X-ray powder diffraction files. The O-to-Fe ratio of the Fe3O4 was determined by scanning electron microscopy-energy dispersive x-ray spectroscopy elemental analysis, and approximated pure Fe3O4. The vibrating sample magnetometer hysteresis loop suggested that the Fe3O4 nanoparticles were superparamagnetic at room temperature. MTT experiments showed that the toxicity of the material

  8. Biocompatibility of Poly (L-Lactic Acid Synthesized In Polymerization Unit By Cytotoxicity And Hemocompatibility Assay And Nanofibers Production

    Directory of Open Access Journals (Sweden)

    Xavier, M.V

    2016-07-01

    Full Text Available The absorbable polyacid is one of the most used and studied materials in tissue engineering. This work synthesized a poly (L-lactic acid (PLLA through ring-opening polymerization and produced nanofibers by the electrospinning process. The PLLA was analyzed by FTIR and the cytotoxicity was evaluated by the MTT assay and Live/Dead®. The hemocompatibility was tested by platelet adhesion and hemolytic activity assay. The tests were performed in contact with human mesenchymal cells at varying times. The high rates of cell viability and proliferation shown by MTT and Live/Dead® tests demonstrate that this PLLA is a non-toxic material and the hemocompatibility assay revealed that the biomaterial was also biocompatible. It was achieved as well the successful production of electrospinning nanofibers, which can be converted for specific biomedical applications in the future

  9. The Blood Compatibilities of Blood Purification Membranes and Other Materials Developed in Japan

    Directory of Open Access Journals (Sweden)

    Takaya Abe

    2011-01-01

    Full Text Available The biocompatibilities in blood purification therapy are defined as “a concept to stipulate safety of blood purification therapy by an index based on interaction in the body arising from blood purification therapy itself.” The biocompatibilities are associated with not only materials to be used but also many factors such as sterilization method and eluted substance. It is often evaluated based on impacts on cellular pathways and on humoral pathways. Since the biocompatibilities of blood purification therapy in particular hemodialysis are not just a prognostic factor for dialysis patients but a contributory factor for long-term complications, it should be considered with adequate attention. It is important that blood purification therapy should be performed by consistently evaluating not only risks associated with these biocompatibilities but also the other advantages obtained from treatments. In this paper, the biocompatibilities of membrane and adsorption material based on Japanese original which are used for blood purification therapy are described.

  10. Modified surface morphology of a novel Ti-24Nb-4Zr-7.9Sn titanium alloy via anodic oxidation for enhanced interfacial biocompatibility and osseointegration.

    Science.gov (United States)

    Li, Xiang; Chen, Tao; Hu, Jing; Li, Shujun; Zou, Qin; Li, Yunfeng; Jiang, Nan; Li, Hui; Li, Jihua

    2016-08-01

    The Ti-24Nb-4Zr-7.9Sn titanium alloy (Ti2448) has shown potential for use in biomedical implants, because this alloy possesses several important mechanical properties, such as a high fracture strength, low elastic modulus, and good corrosion resistance. In this study, we aimed to produce a hierarchical nanostructure on the surface of Ti2448 to endow this alloy with favorable biological properties. The chemical composition of Ti2448 (64.0wt% Ti, 23.9wt% Nb, 3.9wt% Zr, and 8.1wt% Sn) gives this material electrochemical properties that lead to the generation of topographical features under standard anodic oxidation. We characterized the surface properties of pure Ti (Ti), nanotube-Ti (NT), Ti2448, and nanotube-Ti2448 (NTi2448) based on surface morphology (scanning electron microscopy and atomic force microscopy), chemical and phase compositions (X-ray diffraction and X-ray photoelectron spectroscopy), and wettability (water contact angle). We evaluated the biocompatibility and osteointegration of implant surfaces by observing the behavior of bone marrow stromal cells (BMSCs) cultured on the surfaces in vitro and conducting histological analysis after in vivo implantation of the modified materials. Our results showed that a hierarchical structure with a nanoscale bone-like layer was achieved along with nanotube formation on the Ti2448 surface. The surface characterization data suggested the superior biocompatibility of the NTi2448 surface in comparison with the Ti, NT, and Ti2448 surfaces. Moreover, the NTi2448 surface showed better biocompatibility for BMSCs in vitro and better osteointegration in vivo. Based on these results, we conclude that anodic oxidation facilitated the formation of a nanoscale bone-like structure and nanotubes on Ti2448. Unlike the modified titanium surfaces developed to date, the NTi2448 surface, which presents both mechanical compatibility and bioactivity, offers excellent biocompatibility and osteointegration, suggesting its potential for

  11. Facile Synthesis, Silanization and Biodistribution of Biocompatible Quantum Dots

    Science.gov (United States)

    Ma, Nan; Marshall, Ann F.; Gambhir, Sanjiv S.

    2016-01-01

    Here we report a facile strategy to the synthesis of silica-coated quantum dots (QDs) for in vivo imaging. All the QD synthesis and silanization steps are conducted in water and methanol under mild conditions without involving any organometallic precursors and high temperature, oxygen-free environments. The as-prepared silica-coated QDs possess high quantum yields and are extremely stable in mouse serum. In addition, the silanization method developed here produces nanoparticles (NPs) with small sizes that are difficult to achieve via conventional silanization methods. The silica coating helps to prevent the exposure of QD surface to the biological milieu and therefore increases the biocompatibility of QDs for in vivo applications. Interestingly, the silica-coated QDs exhibit a different biodistribution pattern than commercially available Invitrogen QD605 (carboxylate) with a similar size and emission wavelength. The Invitrogen QD605 exhibited predominant liver (57.2% ID g-1) and spleen (46.1% ID g-1) uptakes 30 mins after intravenous injection, whereas the silica-coated QDs exhibited much lower liver (16.2% ID g-1) and spleen (3.67% ID g-1) uptakes but higher kidney uptake (8.82% ID g-1), blood retention (15.0% ID g-1) and partial renal clearance. Overall, this straightforward synthetic strategy paves the way for routine and customized synthesis of silica-coated QDs for biological use. PMID:20564726

  12. Synthesis of biocompatible nanoparticle drug complexes for inhibition of mycobacteria

    Science.gov (United States)

    Bhave, Tejashree; Ghoderao, Prachi; Sanghavi, Sonali; Babrekar, Harshada; Bhoraskar, S. V.; Ganesan, V.; Kulkarni, Anjali

    2013-12-01

    Tuberculosis (TB) is one of the most critical infectious diseases affecting the world today. Current TB treatment involves six months long daily administration of four oral doses of antibiotics. Due to severe side effects and the long treatment, a patient's adherence is low and this results in relapse of symptoms causing an alarming increase in the prevalence of multi-drug resistant (MDR) TB. Hence, it is imperative to develop a new drug delivery technology wherein these effects can be reduced. Rifampicin (RIF) is one of the widely used anti-tubercular drugs (ATD). The present study discusses the development of biocompatible nanoparticle-RIF complexes with superior inhibitory activity against both Mycobacterium smegmatis (M. smegmatis) and Mycobacterium tuberculosis (M. tuberculosis). Iron oxide nanoparticles (NPs) synthesized by gas phase condensation and NP-RIF complexes were tested against M. smegmatis SN2 strain as well as M. tuberculosis H37Rv laboratory strain. These complexes showed significantly better inhibition of M. smegmatis SN2 strain at a much lower effective concentration (27.5 μg ml-1) as compared to neat RIF (125 μg ml-1). Similarly M. tuberculosis H37Rv laboratory strain was susceptible to both nanoparticle-RIF complex and neat RIF at a minimum inhibitory concentration of 0.22 and 1 μg ml-1, respectively. Further studies are underway to determine the efficacy of NPs-RIF complexes in clinical isolates of M. tuberculosis as well as MDR isolates.

  13. A new biocompatible nanocomposite as a promising constituent of sunscreens.

    Science.gov (United States)

    Amin, Rehab M; Elfeky, Souad A; Verwanger, Thomas; Krammer, Barbara

    2016-06-01

    Skin naturally uses antioxidants to protect itself from the damaging effects of sunlight. If this is not sufficient, other measures have to be taken. Like this, hydroxyapatite has the potential to be applied as an active constituent of sunscreens since calcium phosphate absorbs in the ultraviolet region (UV). The objective of the present work was to synthesize a hydroxyapatite-ascorbic acid nanocomposite (HAp/AA-NC) as a new biocompatible constituent of sunscreens and to test its efficiency with skin cell models. The synthesized HAp/AA-NC was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, absorption spectrophotometry and X-ray diffraction analysis. The protective effect of the construct was tested with respect to viability and intracellular reactive oxygen species (ROS) generation of primary human dermal fibroblasts (SKIN) and human epidermal keratinocytes (HaCaT). Both cell lines were irradiated with UV light, λmax=254 nm with a fluence of 25 mJ cm(-2) to mimic the effect of UV radiation of sunlight on the skin. Results showed that HAp/AA-NC had a stimulating effect on the cell viability of both, HaCaT and SKIN cells, relative to the irradiated control. Intracellular ROS significantly decreased in UV irradiated cells when treated with HAp/AA-NC. We conclude that the synthesized HAp/AA-NC have been validated in vitro as a skin protector against the harmful effect of UV-induced ROS. PMID:27040194

  14. Conversion of bulk seashells to biocompatible hydroxyapatite for bone implants.

    Science.gov (United States)

    Vecchio, Kenneth S; Zhang, Xing; Massie, Jennifer B; Wang, Mark; Kim, Choll W

    2007-11-01

    Strombus gigas (conch) shells and Tridacna gigas (Giant clam) shells have dense, tailored structures that impart excellent mechanical properties to these shells. In this investigation, conch and clam seashells were converted to hydroxyapatite (HAP) by a hydrothermal method at different temperatures and for different conversion durations. Dense HAP structures were created from these shells throughout the majority of the samples at the relative low temperature of approximately 200 degrees C. The average fracture stress was found to be approximately 137-218MPa for partially converted conch shell samples and approximately 70-150MPa for original and converted clamshell samples, which is close to the mechanical strength of compact human bone. This indicates that the converted shell samples can be used as implants in load-bearing cases. In vivo tests of converted shell samples were performed in rat femoral defects for 6 weeks. The microtomography images at 6 weeks show that the implants did not move, and untreated control defects remain empty with no evidence of a spontaneous fusion. Histological study reveals that there is newly formed bone growing up to and around the implants. There is no evidence of a fibrosis tissue ring around the implants, also indicating that there is no loosening of the implants. In contrast, the untreated controls remain empty with some evidence of a fibrosis ring around the defect hole. These results indicate good biocompatibility and bioactivity of the converted shell implants. PMID:17684000

  15. In Vitro Biocompatibility of Electrospun Chitosan/Collagen Scaffold

    Directory of Open Access Journals (Sweden)

    Peiwei Wang

    2013-01-01

    Full Text Available Chitosan/collagen composite nanofibrous scaffold has been greatly concerned in recent years for its favorable physicochemical properties which mimic the native extracellular matrix (ECM both morphologically and chemically. In a previous study, we had successfully fabricated nanofibrous chitosan/collagen composite by electrospinning. In the present study, we further investigate the biocompatibility of such chitosan/collagen composite nanofiber to be used as scaffolds in vascular tissue engineering. The porcine iliac artery endothelial cells (PIECs were employed for morphogenesis, attachment, proliferation, and phenotypic studies. Four characteristic EC markers, including two types of cell adhesion molecules, one proliferation molecule (PCNA, and one function molecule (p53, were studied by semiquantitative RT-PCR. Results showed that the chitosan/collagen composite nanofibrous scaffold could enhance the attachment, spreading, and proliferation of PIECs and preserve the EC phenotype. Our work provides profound proofs for the applicable potency of scaffolds made from chitosan/collagen composite nanofiber to be used in vascular tissue engineering.

  16. Biocompatibility and Toxicity of Magnetic Nanoparticles in Regenerative Medicine

    Directory of Open Access Journals (Sweden)

    H. Markides

    2012-01-01

    Full Text Available Regenerative medicine is a pioneering field aimed at restoring and regenerating the function of damaged cells, organs and tissues in order to establish normal function. It demands the cross communication of disciplines to develop effective therapeutic stem cell based therapies. Nanotechnology has been instrumental in the development and translation of basic research to the clinically relevant therapies. In particular, magnetic nanoparticles (MNPs have been applied to tag, track and activate stem cells offering an effective means of monitoring in vitro and in vivo behaviour. MNPs are comprised of an iron oxide core with a biocompatible biological polymer. Safety is an issue of constant concern and emphasises on the importance of investigating the issue of toxicity. Any indication of toxicity can ultimately limit the therapeutic efficiency of the therapy. Toxicity is highly dependent on the physical, chemical and structural properties of the MNP itself as well as dose and intended use. Few in vitro studies have reported adverse effects of MNP on cells at in vitro in therapeutic doses. However, long term in vivo studies have not been studied as extensively. This review aims to summarise current research in this topic highlighting commonly used toxicity assays to investigate this.

  17. Biomolecular interaction analysis for carbon nanotubes and for biocompatibility prediction.

    Science.gov (United States)

    Chen, Xiaoping; Fang, Jinzhang; Cheng, Yun; Zheng, Jianhui; Zhang, Jingjing; Chen, Tao; Ruan, Benfang Helen

    2016-07-15

    The interactions between carbon nanotubes (CNTs) and biologics have been commonly studied by various microscopy and spectroscopy methods. We tried biomolecular interaction analysis to measure the kinetic interactions between proteins and CNTs. The analysis demonstrated that wheat germ agglutinin (WGA) and other proteins have high affinity toward carboxylated CNT (f-MWCNT) but essentially no binding to normal CNT (p-MWCNT). The binding of f-MWCNT-protein showed dose dependence, and the observed kinetic constants were in the range of 10(-9) to 10(-11) M with very small off-rates (10(-3) to 10(-7) s(-1)), indicating a relatively tight and stable f-MWCNT-protein complex formation. Interestingly in hemolysis assay, p-MWCNT showed good biocompatibility, f-MWCNT caused 30% hemolysis, but WGA-coated f-MWCNT did not show hemolysis. Furthermore, the f-MWCNT-WGA complex demonstrated enhanced cytotoxicity toward cancer cells, perhaps through the glycoproteins expressed on the cells' surface. Taken together, biomolecular interaction analysis is a precise method that might be useful in evaluating the binding affinity of biologics to CNTs and in predicting biological actions. PMID:27108187

  18. Spheroid model study comparing the biocompatibility of Biodentine and MTA.

    Science.gov (United States)

    Pérard, Matthieu; Le Clerc, Justine; Watrin, Tanguy; Meary, Fleur; Pérez, Fabienne; Tricot-Doleux, Sylvie; Pellen-Mussi, Pascal

    2013-06-01

    The primary objective of this study was to assess the biological effects of a new dentine substitute based on Ca₃SiO₅ (Biodentine™) for use in pulp-capping treatment, on pseudo-odontoblastic (MDPC-23) and pulp (Od-21) cells. The secondary objective was to evaluate the effects of Biodentine and mineral trioxide aggregate (MTA) on gene expression in cultured spheroids. We used the acid phosphatase assay to compare the biocompatibility of Biodentine and MTA. Cell differentiation was investigated by RT-qPCR. We investigated the expression of genes involved in odontogenic differentiation (Runx2), matrix secretion (Col1a1, Spp1) and mineralisation (Alp). ANOVA and PLSD tests were used for data analysis. MDPC-23 cells cultured in the presence of MTA had higher levels of viability than those cultured in the presence of Biodentine and control cells on day 7 (P = 0.0065 and P = 0.0126, respectively). For Od-21 cells, proliferation rates on day 7 were significantly lower in the presence of Biodentine or MTA than for control (P Biodentine and in control cells. Biodentine and MTA may modify the proliferation of pulp cell lines. Their effects may fluctuate over time, depending on the cell line considered. The observed similarity between Biodentine and MTA validates the indication for direct pulp-capping claimed by the manufacturers. PMID:23515903

  19. Effect of plasma energy on enhancing biocompatibility and hemocompatibility of diamond-like carbon film with various titanium concentrations

    International Nuclear Information System (INIS)

    This investigation develops and explores a new method for depositing a DLC film containing titanium. A bioactive DLC film with titanium dopant (Ti-DLC) was formed by co-sputtering. To determine the properties of DLC films with and without Ti, the specimens were evaluated by material analyses and cell culture. The multilayered nanocrystal TiC was embedded in the amorphous DLC matrix. Microtwins were present between TiC and Ti-DLC. They relaxed residual stress and improved the adhesion of Ti-DLC to the TiC film. The Ti-DLC film proliferates more effectively than Ti or DLC, revealing that the biocompatibility of Ti-DLC clearly exceeds that of DLC, Ti and TiC films. The Ti-DLC film proliferates more effectively than Ti, TiC or DLC film, revealing that the biocompatibility of Ti-DLC clearly exceeds that of DLC and Ti film. In addition, the higher deposited plasma energies were, more densification the films were. It is believed that high plasma energy enhanced the film densification, and then improves surface contact area of adsorbing proteins. It is believed that enhancing cell attachment and subsequently inducing cell proliferation and cell differentiation is related with plasma energy during deposition of Ti-DLC films.

  20. Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.

    Science.gov (United States)

    Wu, Chin-San; Hsu, Yi-Chiang; Liao, Hsin-Tzu; Cai, Yu-Xuan

    2015-12-10

    The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study. Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV. Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility. Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation. However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes. Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications. Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes. Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity. PMID:26428145

  1. Characterization and in vitro biocompatibility study of Ti–Si–N nanocomposite coatings developed by using physical vapor deposition

    International Nuclear Information System (INIS)

    Amongst the Ti alloys used as orthopedic implant materials, Ti6Al4V is one of the widely used alloys. Magnetron sputtering was used to deposit nanocomposite coating of Ti–Si–N on the Ti6Al4V substrate at different power and then the coating structure and surface properties were characterized through contact angle measurement, X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). In vitro biocompatibility of the coatings was assessed by using mouse bone marrow mesenchymal stem cells (mBMMSC). Antibacterial studies were performed using Escherichia coli (E. coli) microorganisms. The osteogenic differentiation was also carried out in order to get gene expressions. The AFM results confirmed that the coatings deposited at 120 W was smoother as compared to other coatings developed at different power, along with optimum contact angle, also these coatings showed good antibacterial results. The fluorescent and viability results of 120 W sample confirmed their good biocompatibility as compared to the coatings deposited 20, 40, 60, and 100 W power. Hence, the coating deposited at 120 W exhibit desirable microstructural characteristics beneficial for surface modification of orthopedic implants

  2. Biocompatibility of Nanoporous TiO2 Coating on NiTi Alloy Prepared via Dealloying Method

    Directory of Open Access Journals (Sweden)

    Jin Huang

    2012-01-01

    Full Text Available This paper investigated the biocompatibility of nanoporous TiO2 coating on NiTi shape-memory alloy (SMA prepared via dealloying method. Our previous study shows that the dealloying treatment at low temperature leads to 130 nm Ni-free surface titania surface layer, which possesses good bioactivity because of the combination of hydroxyl (OH− group in the process of dealloying treatment simultaneously. In this paper, the biological compatibility of NiTi alloy before and after dealloying treatment was evaluated and compared by direct contact method with dermal mesenchymal stem cells (DMSCs by the isolated culture way. The interrelation between the biological compatibility and surface change of material after modification was systematically analyzed. As a consequence, the dealloying treatment method at low temperature could be of interest for biomedical application, as it can avoid sensitization and allergies and improve biocompatibility of NiTi shape-memory alloys. Thus it laid the foundation of the clinical trials for surface modification of NiTi memory alloy.

  3. Evaluation of implant calcium-phosphate materials depending on their mineral content

    Directory of Open Access Journals (Sweden)

    I. A. Talashova

    2016-03-01

    Full Text Available Biocompatibility of original implant calcium-phosphate materials was evaluated in the experiment on animals. The methods of radiological electron-probe microanalysis (REMA and light and scan electron microscopy (SEM were used. Studied materials had the properties of biodegradation, osteoinduction and osteoconduction at different extent. The materials with the composite maximally close to the the bone tissue had the greatest grade of biocompatibility.

  4. Development of a discriminatory biocompatibility testing model for non-precious dental casting alloys.

    LENUS (Irish Health Repository)

    McGinley, Emma Louise

    2011-12-01

    To develop an enhanced, reproducible and discriminatory biocompatibility testing model for non-precious dental casting alloys, prepared to a clinically relevant surface finishing condition, using TR146 oral keratinocyte cells.

  5. Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Wang, Zhenyu; Kutter, Jörg Peter; Dufva, Hans Martin; Wolff, Anders

    2006-01-01

    There is an ever increasing need to find surfaces that are biocompatible for applications like medical implants and microfluidics-based cell culture systems. The biocompatibility of five different surfaces with different hydrophobicity was determined using gene expression profiling as well as more...... conventional methods to determine biocompatibility such as cellular growth rate, morphology and the hydrophobicity of the surfaces. HeLa cells grown on polymethylmethacrylate (PMMA) or a SU-8 surface treated with HNO3-ceric ammonium nitrate (HNO3-CAN) and ethanolamine showed no differences in growth rate......, morphology or gene expression profiles as compared to HeLa cells grown in cell culture flasks. Cells grown on SU-8 treated with only HNO3-CAN showed almost the same growth rate (36 ¡ 1 h) and similar morphology as cells grown in cell culture flasks (32 ¡ 1 h), indicating good biocompatibility. However, more...

  6. Light-cured resin "Barricaid" - An aesthetic and biocompatible dressing: A step ahead

    Directory of Open Access Journals (Sweden)

    Ellora Madan

    2013-01-01

    Full Text Available Background: Periodontal dressings have been used for several years as a protection over injured tissue to shield the area from further insult . Several dressings are commercially available. A recently introduced light-cured resin, claimed to be more biocompatible and esthetic, needs critical evaluation. Aim: To compare this dressing with most widely used non-eugenol pack in the perspective of esthetics, acceptance, and healing following periodontal flap surgery. Materials and Methods: Twenty patients suffering from generalized chronic periodontitis, requiring periodontal flap surgery on contralateral sides of the arch, were selected and divided randomly into Group I (control and Group II (test. In Group I, a non-eugenol dressing and in Group II light-cured dressing were applied after flap surgery. Pain and discomfort scores were recorded on day 1, 2, and 3 while plaque scores, gingival index, and bleeding index were recorded on day 7. Patient′s subjective evaluation and preference for the dressing material were recorded. The data was collected and statistically analyzed. Results: Group II showed better results than Group I when plaque scores, bleeding scores, modified gingival index scores, and pain and discomfort scores were compared though the differences were not statistically significant. Subjects found no unpleasant taste/smell and perceived the light-cured dressing to be better. A significantly higher number of patients preferred light-cured resin as a post-surgical dressing over Coe-pak. Conclusion: The light-cured dressing showed better patient acceptability and proves to be a better alternative to Coe-pak as a dressing material.

  7. (α'(H))-Dicalcium silicate bone cement doped with tricalcium phosphate: characterization, bioactivity and biocompatibility.

    Science.gov (United States)

    de Aza, Piedad N; Zuleta, Fausto; Velasquez, Pablo; Vicente-Salar, Nestor; Reig, Juan A

    2014-02-01

    The influence of phosphorus doping on the properties of (α'(H))-dicalcium silicate (C(2)S) bone cement was analyzed, in addition to bioactivity and biocompatibility. All the cements were composed of a solid solution of TCP in C(2)S ([Formula: see text]-C(2)S(ss)) as the only phase present. The compressive strength ranged from 3.8-16.3 MPa. Final setting times ranged from 10 to 50 min and were lower for cements with lower L/P content. Calcium silicate hydrate was the principal phase formed during the hydration process of the cements. The cement exhibited a moderate degradation and could induce carbonated hydroxyapatite formation on its surface and into the pores. The cell attachment test showed that the (α'(H))-C(2)SiO(4) solid solution supported human adipose stem cells adhesion and spreading, and the cells established close contacts with the cement after 24 h of culture. The novel (α'(H))-C(2)S(ss) cements might be suitable for potential applications in the biomedical field, preferentially as materials for bone/dental repair. PMID:24218299

  8. In vitro biocompatibility of titanium after plasma surface alloying with boron.

    Science.gov (United States)

    Kaczmarek, Mariusz; Jurczyk, Mieczysława U; Miklaszewski, Andrzej; Paszel-Jaworska, Anna; Romaniuk, Aleksandra; Lipińska, Natalia; Żurawski, Jakub; Urbaniak, Paulina; Jurczyk, Karolina

    2016-12-01

    Recently, the effect of different sizes of precursor powders during surface plasma alloying modification on the properties of titanium surface was studied. In this work we show in vitro test results of the titanium (α-Ti) after plasma surface alloying with boron (B). Ti-B nanopowders with 2 and 10wt% B were deposited onto microcrystalline Ti substrate. The in vitro cytocompatibility of these biomaterials was evaluated and compared with a conventional microcrystalline Ti. During the studies, established cell line of human gingival fibroblasts and osteoblasts were cultured in the presence of tested materials, and its survival rate and proliferation activity were examined. For this purpose, MTT assay, flow cytometric and fluorescent microscopic evaluation were made. Biocompatibility tests carried out indicate that the Ti after plasma surface alloying with B could be a possible candidate for dental implants and other medicinal applications. Plasma alloying is a promising method for improving the properties of titanium, thus increasing the field of its applications. PMID:27612822

  9. In vivo biocompatibility of custom-fabricated apatite-wollastonite-mesenchymal stromal cell constructs.

    Science.gov (United States)

    Lee, Jennifer A; Knight, Charlotte A; Kun, Xiao; Yang, Xuebin B; Wood, David J; Dalgarno, Kenneth W; Genever, Paul G

    2015-10-01

    We have used the additive manufacturing technology of selective laser sintering (SLS), together with post SLS heat treatment, to produce porous three dimensional scaffolds from the glass-ceramic apatite-wollastonite (A-W). The A-W scaffolds were custom-designed to incorporate a cylindrical central channel to increase cell penetration and medium flow to the center of the scaffolds under dynamic culture conditions during in vitro testing and subsequent in vivo implantation. The scaffolds were seeded with human bone marrow mesenchymal stromal cells (MSCs) and cultured in spinner flasks. Using confocal and scanning electron microscopy, we demonstrated that MSCs formed and maintained a confluent layer of viable cells on all surfaces of the A-W scaffolds during dynamic culture. MSC-seeded, with and without osteogenic pre-differentiation, and unseeded A-W scaffolds were implanted subcutaneously in MF1 nude mice where osteoid formation and tissue in-growth were observed following histological assessment. The results demonstrate that the in vivo biocompatibility and osteo-supportive capacity of A-W scaffolds can be enhanced by SLS-custom design, without the requirement for osteogenic pre-induction, to advance their potential as patient-specific bone replacement materials. PMID:25777813

  10. Robust and Biocompatible Hybrid Matrix with Controllable Permeability for Microalgae Encapsulation.

    Science.gov (United States)

    Zhang, Bo-Bo; Wang, Li; Charles, Valérie; Rooke, Joanna C; Su, Bao-Lian

    2016-04-13

    Hybrid beads with entrapped microalgae Chlamydomonas reinhardtii were synthesized for the sustainable production of high value metabolites via photosynthesis. Encapsulating the microalgae requires an exquisite control of material properties, which has been achieved by modifying the composition (alginate, polycation, and silica). A coating of PDADMAC precluded cell leakage as indicated by the OD750 value of the culture medium, and the homogeneous distribution of silica prevented bead shrinkage from the strong electronic force of PDADMAC, resulting in a robust and biocompatible matrix for the cells. Besides fabricating suitable porous beads for the diffusion of expected metabolites, the permeability can be controlled to a certain degree by applying different molecular weights of PDADMAC. The hybrid alginate+silica/CaCl2+PDADMAC beads possessed sufficient mechanical rigidity to sheer force under constant stirring and good chemical stability to chelating agents such as sodium citrate. Moreover, the encapsulated cells exhibited excellent long-term viability and cellular functionality, which retained about 81.5% of the original value after a 120 day encapsulation as observed by microscopy and oximetry measurement. This study is not only significant for understanding the critical role of polycations and silica involved in the synthesis of hybrid beads but also important for real-scale bioengineering applications. PMID:27027232

  11. Effect of the deposition temperature on corrosion resistance and biocompatibility of the hydroxyapatite coatings

    Science.gov (United States)

    Vladescu, A.; Braic, M.; Azem, F. Ak; Titorencu, I.; Braic, V.; Pruna, V.; Kiss, A.; Parau, A. C.; Birlik, I.

    2015-11-01

    Hydroxyapatite (HAP) ceramics belong to a class of calcium phosphate-based materials, which have been widely used as coatings on titanium medical implants in order to improve bone fixation and thus to increase the lifetime of the implant. In this study, HAP coatings were deposited from pure HAP targets on Ti6Al4V substrates using the radio-frequency magnetron sputtering technique at substrate temperatures ranging from 400 to 800 °C. The surface morphology and the crystallographic structure of the films were investigated by atomic force microscopy (AFM), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The corrosion resistance of the coatings in saliva solution at 37 °C was evaluated by potentiodynamic polarization. Additionally, the human osteosarcoma cell line (MG-63) was used to test the biocompatibility of the coatings. The results showed that all of the coatings grown uniformly and that the increasing substrate temperature induced an increase in their crystallinity. Corrosion performance of the coatings was improved with the increase of the substrate temperature from 400 °C to 800 °C. Furthermore, all the coatings support the attachment and growth of the osteosarcoma cells with regard to the in vitro test findings.

  12. Application of Polyethylene Glycol to Promote Cellular Biocompatibility of Polyhydroxybutyrate Films

    Directory of Open Access Journals (Sweden)

    Rodman T. H. Chan

    2011-01-01

    Full Text Available Polyhydroxybutyrate (PHB is a biomaterial with potential for applications in biomedical and tissue engineering; however, its brittle nature and high crystallinity limit its potential. Blending PHB with a variety of PEGs produced natural-synthetic composite films composed of FDA-approved polymers with significant reductions in crystallinity, from 70.1% for PHB films to 41.5% for its composite with a 30% (w/w loading of PEG2000. Blending also enabled manipulation of the material properties, increasing film flexibility with an extension to break of 2.49±1.01% for PHB films and 8.32±1.06% for films containing 30% (w/w PEG106. Significant changes in the film surface properties, as measured by porosity, contact angles, and water uptake, were also determined as a consequence of the blending process, and these supported greater adhesion and proliferation of neural-associated olfactory ensheathing cells (OECs. A growth rate of 7.2×105 cells per day for PHB films with 30% (w/w PEG2000 loading compared to 2.5×105 for PHB films was observed. Furthermore, while cytotoxicity of the films as measured by lactate dehydrogenase release was unaffected, biocompatibility, as measured by mitochondrial activity, was found to increase. It is anticipated that fine control of PEG composition in PHB-based composite biomaterials can be utilised to support their applications in medicinal and tissue engineering applications.

  13. Controllable fabrication and characterization of biocompatible core-shell particles and hollow capsules as drug carrier

    Science.gov (United States)

    Hao, Lingyun; Gong, Xinglong; Xuan, Shouhu; Zhang, Hong; Gong, Xiuqing; Jiang, Wanquan; Chen, Zuyao

    2006-10-01

    SiO 2@CdSe core-shell particles were fabricated by controllable deposition CdSe nanoparticles on silica colloidal spheres. Step-wise coating process was tracked by the TEM and XRD measurements. In addition, SiO 2@CdSe/polypyrrole(PPy) multi-composite particles were synthesized based on the as-prepared SiO 2@CdSe particles by cationic polymerization. The direct electrochemistry of myoglobin (Mb) could be performed by immobilizing Mb on the surface of SiO 2@CdSe particles. Immobilized with Mb, SiO 2@CdSe/PPy-Mb also displayed good bioelectrochemical activity. It confirmed the good biocompatible property of the materials with protein. CdSe hollow capsules were further obtained as the removal of the cores of SiO 2@CdSe spheres. Hollow and porous character of CdSe sub-meter size capsules made them becoming hopeful candidates as drug carriers. Doxorubicin, a typical an antineoplastic drug, was introduced into the capsules. A good sustained drug release behavior of the loading capsules was discovered via performing a release test in the PBS buffer (pH 7.4) solution at 310 k. Furthermore, SiO 2@CdSe/PPy could be converted to various smart hollow capsules via selectively removal of their relevant components.

  14. CHARACTERIZATION OF YTTRIA AND MAGNESIA PARTIALLY STABILIZED ZIRCONIA BIOCOMPATIBLE COATINGS DEPOSITED BY PLASMA SPRAYING

    Directory of Open Access Journals (Sweden)

    Roşu R. A.

    2013-09-01

    Full Text Available Zirconia (ZrO2 is a biocompatible ceramic material which is successfully used in medicine to cover the metallic implants by various methods. In order to avoid the inconvenients related to structural changes which may appear because of the temperature treatment while depositing the zirconia layer over the metallic implant, certain oxides are added, the most used being Y2O3, MgO and CaO. This paper presents the experimental results regarding the deposition of yttria (Y2O3 and magnesia (MgO partially stabilized zirconia layers onto titanium alloy substrate by plasma spraying method. X ray diffraction investigations carried out both on the initial powders and the coatings evidenced the fact that during the thermal spraying process the structure has not been significantly modified, consisting primarily of zirconium oxide with tetragonal structure. Electronic microscopy analyses show that the coatings are dense, uniform and cracks-free. Adherence tests performed on samples whose thickness ranges between 160 and 220 μm showed that the highest value (23.5 MPa was obtained for the coating of ZrO2 - 8 wt. % Y2O3 with 160 μm thickness. The roughness values present an increasing tendency with increasing the coatings thickness.

  15. Biocompatibility Evaluation of Vessel Extracellular Matrix as a Matrix for Urethral Reconstruction

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The objective of this study was to evaluate the biocompatibility of vessel extracellular matrix (VECM) from rabbit and to discuss the feasibility of vessel extracellular matrix as a matrix for urethral reconstruction. Primary cultured bladder smooth muscle cells isolated from New Zealand rabbits were implanted on VECM .The effects of VECM on rabbit bladder smooth muscle cells (RBSMCs) metabolic activity,attachment, proliferation were monitored in vitro with the aid of an inverted light microscope and a scanning electron microscope. The cell viability was monitored by MTT(methythiazolye tetrazolium bromide) after 1, 3,5 days seeding. The in vivo tissue response to VECM was investigated by implanting them into the subcutaneous of rabbits. VECM exhibited a nontoxic and bioactive effect on RBSMCs. RBSMCs could be attached to and proliferated on VECM and maintained their morphologies. MTT assay showed RBSMCs cultured with the extracts of VECM were not significantly different from those of negative controls. In vivo, VECM demonstrated a favorable tissue compatibility without tissue necrosis, fibrosis and other abnormal response. VECM exhibited nontoxic and bioactive effects on RBSMC. It is a suitable material for urethral reconstruction.

  16. Effect of a cordless retraction paste on titanium surface: a topographic, chemical and biocompatibility evaluation

    Directory of Open Access Journals (Sweden)

    Katherine Cooper

    2013-06-01

    Full Text Available Good exposure of the preparation margins and haemostasis in the sulcular gingiva are necessary for accurate impressions to produce precise restorations. The use of cordless retraction paste material in implant dentistry is a relatively novel application. However, few studies have been conducted on the use of retraction pastes and their possible interaction with implant surfaces. Recent literature has described remnants on titanium implant surfaces and expressed the need for an assessment of the biocompatibility of the exposed surface (Chang et al.. This in vitro study evaluated the effect of a cordless gingival retraction paste on sterile titanium disks. Surface chemistry was determined using energy-dispersive X-ray spectroscopy (EDS, and further investigated using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS. After exposure to retraction paste, surface chemistry alterations were identified. A fibroblast cell line (L929 was exposed to the disks and the live/dead viability/cytotoxicity assay was used to determine any effects on the proliferation and health of cells. The disks exposed to the retraction paste showed fewer dead cells compared to the unexposed disks. This was statistically significant.

  17. The impact of zirconium oxide nanoparticles on the hydration chemistry and biocompatibility of white Portland cement.

    Science.gov (United States)

    Li, Qiu; Deacon, Andrew D; Coleman, Nichola J

    2013-01-01

    Zirconium oxide (ZrO2) has been nominated as a radiopacifying agent for use in MTA-like Portland cement-based root-filling materials. This research examines the impact of 20 wt% ZrO2 nanoparticles in the size range 50 to 75 nm on the early hydration chemistry of white Portland cement. Nano-ZrO2 was found to accelerate the degree of hydration by 26% within the first 24 h by presenting efficient nucleation sites for the precipitation and growth of the early C-S-H gel products. The presence of nano-ZrO2 was also found to divert the fate of the aluminium-bearing reaction products by lowering the ettringite to monosulphate ratio, reducing the size of the ettringite crystals and by increasing the Al:Si ratio of the C-S-H gel phase. The chemical and microstructural changes conferred upon the cement matrix by the nano-ZrO2 particles had a positive impact on in vitro biocompatibility with respect to MG63 osteosarcoma cells (via MTT assay). PMID:24088838

  18. New Biocompatible Polyesters Derived from α-Amino Acids: Hydrolytic Degradation Behavior

    Directory of Open Access Journals (Sweden)

    Jeoshua Katzhendler

    2010-10-01

    Full Text Available New polymers were synthesized from α-hydroxy acids derived from the natural amino acids Ile, Leu, Phe, and Val, combined with lactic acid, glycolic acid and 6-hydroxyhexanoic acid by direct condensation. The toxicity was determined and the degradation process of these polyesters was investigated under physiological conditions by analyzing the composition of the degraded polymers and the oligomers cleaved in the buffer medium. The polymers were found to be non toxic to two cell lines. Polymers displayed a biphasic degradation behavior. In most cases, a linear relationship was found between the weight loss constant and the hydrophobicity of the polymers, Log P. Regarding the second stage of weight loss, it is apparent that polymers derived from α-hydroxy(Lisoleucine ((LHOIle and α-hydroxy(LValine ((LHOVal degraded much faster than those derived from α-hydroxy(Lleucine ((LHOLeu and α-hydroxy(Lphenylalanine ((LHOPhe, probably due to different spatial orientation of the side chains. Copolymers of 6-hydroxyhexanoic acid displayed slow degradation rates as expected, whereas the degradation profile of copolymers of lactic acid was similar to the other homopolymers. These new polyesters may serve as potential biocompatible materials for medical applications.

  19. Tribological Study of Biocompatible Hybrid Organic Molecules Film with Antibacterial Effect

    Directory of Open Access Journals (Sweden)

    J.H. Horng

    2013-06-01

    Full Text Available Optical glass is widely used in bioengineering and various utilities such as public touchscreen displays and mobile devices. This work evaluates the features of anti‐bacterial and anti‐adhesion on Octadecyltrichlorosilane (OTS material that was mixed with a biocompatible antibacterial agent coated on the optical glass. Test samples were allocated to different bath and drying temperatures as well as reaction times. Results show that in angle contact experiments, pure OTS films and mixed antibacterial films have almost the same contact angle of about 105° under the conditions of a 12 hour reaction time and 80 °C reaction temperature. The antibacterial test indicated the following order: antibacterial agent> OTS+ antibacterialagent (50 % > OTS+ antibacterial agent (10 % > OTS. At the sameoperation condition, OTS mixed with 50 % antibacterial agent was able to increase the adhesion force between the OTS film and lens. This suggests that surface treatment of optical lenses involving OTS with 50 % antibacterial solution is the most effective for increasing antifouling and antibacterial functions while simultaneously‐ enhancing the adhesion function between films and lens surfaces.

  20. Effect of a cordless retraction paste on titanium surface: a topographic, chemical and biocompatibility evaluation.

    Science.gov (United States)

    Cooper, Katherine; Bennani, Vincent; Tawse-Smith, Andrew; Reid, Malcolm; Stirling, Claudine; Dias, George

    2013-01-01

    Good exposure of the preparation margins and haemostasis in the sulcular gingiva are necessary for accurate impressions to produce precise restorations. The use of cordless retraction paste material in implant dentistry is a relatively novel application. However, few studies have been conducted on the use of retraction pastes and their possible interaction with implant surfaces. Recent literature has described remnants on titanium implant surfaces and expressed the need for an assessment of the biocompatibility of the exposed surface (Chang et al.). This in vitro study evaluated the effect of a cordless gingival retraction paste on sterile titanium disks. Surface chemistry was determined using energy-dispersive X-ray spectroscopy (EDS), and further investigated using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). After exposure to retraction paste, surface chemistry alterations were identified. A fibroblast cell line (L929) was exposed to the disks and the live/dead viability/cytotoxicity assay was used to determine any effects on the proliferation and health of cells. The disks exposed to the retraction paste showed fewer dead cells compared to the unexposed disks. This was statistically significant. PMID:23739788

  1. Evaluation of Synthesized Nanohydroxyapatite-Nanocellulose Composites as Biocompatible Scaffolds for Applications in Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Claudia S. Herdocia-Lluberes

    2015-01-01

    Full Text Available Basic calcium phosphate (BCP crystals have been associated with many diseases due to their activation of signaling pathways that lead to their mineralization and deposition in intra-articular and periarticular locations in the bones. In this study, hydroxyapatite (HAp has been placed in a polysaccharide network as a strategy to minimize this deposition. This research consisted of the evaluation of varying proportions of the polysaccharide network, cellulose nanocrystals (CNCs, and HAp synthesized via a simple sol-gel method. The resulting biocompatible composites were extensively characterized by means of thermogravimetric analysis (TGA, powder X-ray diffraction (XRD, Fourier transform infrared spectroscopy (FT-IR, dynamic light scattering (DLS, zeta potential, and scanning electron microscopy (SEM. It was found that an nHAp = CNC ratio presented greater homogeneity in the size and distribution of the nanoparticles without compromising the crystalline structure. Also, incorporation of bone morphogenetic protein 2 (BMP-2 was performed to evaluate the effects that this interaction would have in the constructs. Finally, the osteoblast cell (hFOB 1.19 viability assay was executed and it showed that all of the materials promoted greater cell proliferation while the nHAp > CNC proportion with the inclusion of the BMP-2 protein was the best composite for the purpose of this study.

  2. Biocompatible circuit-breaker chip for thermal management of biomedical microsystems

    International Nuclear Information System (INIS)

    This paper presents a thermoresponsive micro circuit breaker for biomedical applications specifically targeted at electronic intelligent implants. The circuit breaker is micromachined to have a shape-memory-alloy cantilever actuator as a normally closed temperature-sensitive switch to protect the device of interest from overheating, a critical safety feature for smart implants including those that are electrothermally driven with wireless micro heaters. The device is fabricated in a size of 1.5  ×  2.0  ×  0.46 mm3 using biocompatible materials and a chip-based titanium package, exhibiting a nominal cold-state resistance of 14 Ω. The breaker rapidly enters the full open condition when the chip temperature exceeds 63 °C, temporarily breaking the circuit of interest to lower its temperature until chip temperature drops to 51 °C, at which the breaker closes the circuit to allow current to flow through it again, physically limiting the maximum temperature of the circuit. This functionality is tested in combination with a wireless resonant heater powered by radio-frequency electromagnetic radiation, demonstrating self-regulation of heater temperature. The developed circuit-breaker chip operates in a fully passive manner that removes the need for active sensor and circuitry to achieve temperature regulation in a target device, contributing to the miniaturization of biomedical microsystems including electronic smart implants where thermal management is essential. (paper)

  3. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

    Directory of Open Access Journals (Sweden)

    Kewalin Inthanon

    2016-01-01

    Full Text Available Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone-sericin (PLCL-SC copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35–40% higher than those of a standard PLCL and commercial polystyrene (PS. The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N–H, and C–N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton’s jelly mesenchymal stem cells (hWJMSC proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK. On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.

  4. Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton's Jelly Mesenchymal Stem Cells.

    Science.gov (United States)

    Inthanon, Kewalin; Daranarong, Donraporn; Techaikool, Pimwalan; Punyodom, Winita; Khaniyao, Vorathep; Bernstein, Audrey M; Wongkham, Weerah

    2016-01-01

    Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35-40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N-H, and C-N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications. PMID:26839562

  5. Preparation of highly luminescent and biocompatible carbon dots using a new extraction method

    International Nuclear Information System (INIS)

    C dots (CDs) are among the most promising emerging fluorescent labels for biological imaging and sensing. A facile new synthesis method was developed using common organic solvents for fabricating CDs from candle soot. The common organic solvents were used as extractants and the obtained CDs have a narrow size distribution with average diameters of about 3.4 nm for ethylene glycol, 3.5 nm for ethanol, and 3.4 nm for n-butanol. This approach is simpler, easier, and more effective than other methods currently used for CD fabrication. The obtained CDs had a high quantum yield (38 %), tunable emission and are water-soluble. The mechanism for the luminescence of the CDs was investigated and the results indicate that the ability of the solvent to disperse the CDs plays a very important role in the photoluminescence of these CDs. The type of organic solvent and the surface groups on the CDs also influenced the optical properties of the CDs. Different emissive traps are shown to play the major role in the luminescence of the carbon materials. An in vitro hemolysis assay was performed and showed that the CDs are biocompatible

  6. Hydroxyapatite coating on magnesium with MgF₂ interlayer for enhanced corrosion resistance and biocompatibility.

    Science.gov (United States)

    Jo, Ji-Hoon; Kang, Bong-Gyu; Shin, Kwang-Seon; Kim, Hyoun-Ee; Hahn, Byung-Dong; Park, Dong-Soo; Koh, Young-Hag

    2011-11-01

    Hydroxyapatite (HA) was coated onto pure magnesium (Mg) with an MgF(2) interlayer in order to reduce the surface corrosion rate and enhance the biocompatibility. Both MgF(2) and HA were successfully coated in sequence with good adhesion properties using the fluoride conversion coating and aerosol deposition techniques, respectively. In a simulated body fluid (SBF), the double layer coating remarkably enhanced the corrosion resistance of the coated Mg specimen. The in vitro cellular responses of the MC3T3-E1 pre-osteoblasts were examined using a cell proliferation assay and an alkaline phosphatase (ALP) assay, and these results demonstrated that the double coating layer also enhanced cell proliferation and differentiation levels. In the in vivo study, the HA/MgF(2) coated Mg corroded less than the bare Mg and had a higher bone-to-implant contact (BIC) ratio in the cortical bone area of the rabbit femora 4 weeks after implantation. These in vitro and in vivo results suggested that the HA coated Mg with the MgF(2) interlayer could be used as a potential candidate for biodegradable implant materials. PMID:21909643

  7. Ionic liquid based polymeric liposomes: A stable and biocompatible soft platform for bioelectrochemistry.

    Science.gov (United States)

    Tian, Yanping; Xia, Jiarui; Zhang, Ling; Zhang, Junhui; Jiang, Yi; Zhang, Yao; Yang, Lini; Zhang, Qian; Xia, Lixin

    2016-10-01

    Polymeric liposomes (denoted as ILs-polysomes) are a biocompatible and conductive nanomaterial, which was first utilised as the electrode material for immobilising and biosensing redox enzyme horseradish peroxide (HRP). The morphology and surface property of IL-polysomes was characterised and systematically compared with unpolymerised ionic liquid based liposomes (denoted as ILs-liposomes). Differing from IL-liposomes, IL-polysomes preserves their original morphology and bilayer membrane structure on glassy carbon (GC) electrodes due to the cross-linking of polymerised lipids, thus exhibiting excellent stability and specific biocompability. Because of the existence of imidazolium ionic liquid moieties on the outer surface, IL-polysomes displays a positive charge in aqueous solution, leading to oppositely charged HRP self-assembling onto the vesicles to form HRP/IL-polysomes/PVA/GC electrodes. Owing to the combined merits of ILs and liposomes, electron transfer between HRP-Fe(III)/Fe(II) redox couples of immobilised enzymes and GC electrodes can be achieved. Therefore, HRP/IL-polysomes/PVA/GC electrodes exhibited good electrocatalytic performance toward the electrocatalysis of H2O2. Accordingly, IL-polysomes could act as an efficient charged platform for the self-assembled redox enzymes to realise direct electrochemistry. IL-polysomes have a promising application in the fabrication of third-generation electrochemical biosensors. PMID:27196632

  8. Mechanical Strength and Biocompatibility of Ultrafine-Grained Commercial Purity Titanium

    OpenAIRE

    Yuri Estrin; Hyoun-Ee Kim; Rimma Lapovok; Hoi Pang Ng; Ji-Hoon Jo

    2013-01-01

    The effect of grain refinement of commercial purity titanium by equal channel angular pressing (ECAP) on its mechanical performance and bone tissue regeneration is reported. In vivo studies conducted on New Zealand white rabbits did not show an enhancement of biocompatibility of ECAP-modified titanium found earlier by in vitro testing. However, the observed combination of outstanding mechanical properties achieved by ECAP without a loss of biocompatibility suggests that this is a very promisi...

  9. Nanoclusters of iron oxide: effect of core composition on structure, biocompatibility and cell labeling efficacy

    OpenAIRE

    van Tilborg, Geralda A. F.; Cormode, David P.; Jarzyna, Peter A.; van der Toorn, Annette; van der Pol, Susanne M. A.; van Bloois, Louis; Fayad, Zahi A.; Storm, Gert; Mulder, Willem J. M.; de Vries, Helga E.; Dijkhuizen, Rick M.

    2012-01-01

    Inorganic nanocrystals have a variety of applications in medicine. They may serve as contrast agents, therapeutics and for in vitro diagnostics. Frequently, the synthesis route yields hydrophobically capped nanocrystals, which necessitates their subsequent coating to render a water-soluble and biocompatible probe. Biocompatibility is crucial for cellular imaging applications, which require large quantities of diagnostically active nanoparticles to be loaded into cells. We have previously repo...

  10. Bioinspired, Ultrastrong, Highly Biocompatible, and Bioactive Natural Polymer/Graphene Oxide Nanocomposite Films.

    Science.gov (United States)

    Zhu, Wen-Kun; Cong, Huai-Ping; Yao, Hong-Bin; Mao, Li-Bo; Asiri, Abdullah M; Alamry, Khalid A; Marwani, Hadi M; Yu, Shu-Hong

    2015-09-01

    Tough and biocompatible nanocomposite films: A new type of bioinspired ultrastrong, highly biocompatible, and bioactive konjac glucomannan (KGM)/graphene oxide (GO) nanocomposite film is fabricated on a large scale by a simple solution-casting method. Such KGM-GO composite films exhibit much enhanced mechanical properties under the strong hydrogen-bonding interactions, showing great potential in the fields of tissue engineering and food package. PMID:26097134

  11. Assembly-Controlled Biocompatible Interface on a Microchip: Strategy to Highly Efficient Proteolysis

    OpenAIRE

    Liu, Y; Zhong, W.; Meng, S; Kong, J.; Lu, H.; Yang, P.; Girault, HH; Liu, B

    2006-01-01

    A biocompatible interface was constructed on a microchip by using the layer-by-layer (LBL) assembly of charged polysaccharides incorporating proteases for highly efficient proteolysis. The controlled assembly of natural polyelectrolytes and the enzyme-adsorption step were monitored by using a quartz-crystal microbalance and atomic force microscopy (AFM). Such a multilayer-assembled membrane provides a biocompatible interconnected network with high enzyme-loading capacity. The maximum digest...

  12. In Vitro Models in Biocompatibility Assessment for Biomedical-Grade Chitosan Derivatives in Wound Management

    OpenAIRE

    Lim Chin Keong; Ahmad Sukari Halim

    2009-01-01

    One of the ultimate goals of wound healing research is to find effective healing techniques that utilize the regeneration of similar tissues. This involves the modification of various wound dressing biomaterials for proper wound management. The biopolymer chitosan (b-1,4-D-glucosamine) has natural biocompatibility and biodegradability that render it suitable for wound management. By definition, a biocompatible biomaterial does not have toxic or injurious effects on biological systems. Chemica...

  13. Graphene Films Show Stable Cell Attachment and Biocompatibility with Electrogenic Primary Cardiac Cells

    OpenAIRE

    Kim, Taeyong; Kahng, Yung Ho; Lee, Takhee; Lee, Kwanghee; Kim, Do Han

    2013-01-01

    Graphene has attracted substantial attention due to its advantageous materialistic applicability. In the present study, we tested the biocompatibility of graphene films synthesized by chemical vapor deposition with electrogenic primary adult cardiac cells (cardiomyocytes) by measuring the cell properties such as cell attachment, survival, contractility and calcium transients. The results show that the graphene films showed stable cell attachment and excellent biocompatibility with the electro...

  14. In Vivo Biocompatibility of PLGA-Polyhexylthiophene Nanofiber Scaffolds in a Rat Model

    OpenAIRE

    Swaminathan Sethuraman; Anuradha Subramanian; Uma Maheswari Krishnan

    2013-01-01

    Electroactive polymers have applications in tissue engineering as a physical template for cell adhesion and carry electrical signals to improve tissue regeneration. Present study demonstrated the biocompatibility and biodegradability of poly(lactide-co-glycolide)-poly(3-hexylthiophene) (PLGA-PHT) blend electrospun scaffolds in a subcutaneous rat model. The biocompatibility of PLGA-undoped PHT, PLGA-doped PHT, and aligned PLGA-doped PHT nanofibers was evaluated and compared with random PLGA fi...

  15. An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones

    OpenAIRE

    Boos Alois; Auer Joerg A; Nuss Katja; Rechenberg Brigitte von

    2006-01-01

    Abstract Background The past years have seen the development of many synthetic bone replacements. To test their biocompatibility and ability for osseointegration, osseoinduction and -conduction requires their placement within bone preferably in an animal experiment of a higher species. Methods A suitable experimental animal model in sheep with drill holes of 8 mm diameter and 13 mm depth within the proximal and distal humerus and femur for testing biocompatibility issues is introduced. Result...

  16. In Vitro Models in BiocompatibilityAssessment for Biomedical-Grade Chitosan Derivatives in Wound Management

    Directory of Open Access Journals (Sweden)

    Lim Chin Keong

    2009-03-01

    Full Text Available One of the ultimate goals of wound healing research is to find effective healing techniques that utilize the regeneration of similar tissues. This involves the modification of various wound dressing biomaterials for proper wound management. The biopolymer chitosan (b-1,4-D-glucosamine has natural biocompatibility and biodegradability that render it suitable for wound management. By definition, a biocompatible biomaterial does not have toxic or injurious effects on biological systems. Chemical and physical modifications of chitosan influence its biocompatibility and biodegradability to an uncertain degree. Hence, the modified biomedical-grade of chitosan derivatives should be pre-examined in vitro in order to produce high-quality, biocompatible dressings. In vitro toxicity examinations are more favorable than those performed in vivo, as the results are more reproducible and predictive. In this paper, basic in vitro tools were used to evaluate cellular and molecular responses with regard to the biocompatibility of biomedical-grade chitosan. Three paramount experimental parameters of biocompatibility in vitro namely cytocompatibility, genotoxicity and skin pro-inflammatory cytokine expression, were generally reviewed for biomedical-grade chitosan as wound dressing.

  17. Highly biocompatible, nanocrystalline hydroxyapatite synthesized in a solvothermal process driven by high energy density microwave radiation

    Directory of Open Access Journals (Sweden)

    Smolen D

    2013-02-01

    Full Text Available Dariusz Smolen1, Tadeusz Chudoba1, Iwona Malka1, Aleksandra Kedzierska1, Witold Lojkowski1, Wojciech Swieszkowski2, Krzysztof Jan Kurzydlowski2, Malgorzata Kolodziejczyk-Mierzynska3, Malgorzata Lewandowska-Szumiel31Polish Academy of Science, Institute of High Pressure Physics, Warsaw, Poland; 2Faculty of Materials Engineering, Warsaw University of Technology, Warsaw, Poland; 3Department of Histology and Embryology, Center of Biostructure Research, Medical University of Warsaw, Warsaw, PolandAbstract: A microwave, solvothermal synthesis of highly biocompatible hydroxyapatite (HAp nanopowder was developed. The process was conducted in a microwave radiation field having a high energy density of 5 W/mL and over a time less than 2 minutes. The sample measurements included: powder X-ray diffraction, density, specific surface area, and chemical composition. The morphology and structure were investigated by scanning electron microscopy as well as transmission electron microscopy (TEM. The thermal behavior analysis was conducted using a simultaneous thermal analysis technique coupled with quadruple mass spectrometry. Additionally, Fourier transform infrared spectroscopy tests of heated samples were performed. A degradation test and a biocompatibility study in vitro using human osteoblast cells were also conducted. The developed method enables the synthesis of pure, fully crystalline hexagonal HAp nanopowder with a specific surface area close to 240 m2/g and a Ca/P molar ratio equal to 1.57. TEM measurements showed that this method results in particles with an average grain size below 6 nm. A 28-day degradation test conducted according to the ISO standard indicated a 22% loss of initial weight and a calcium ion concentration at 200 µmol/dm3 in the tris(hydroxymethylaminomethane hydrochloride test solution. The cytocompatibility of the obtained material was confirmed in a culture of human bone derived cells, both in an indirect test using the material

  18. Comparison of the biocompatibility of different root canal irrigants

    Directory of Open Access Journals (Sweden)

    João Eduardo Gomes-Filho

    2008-04-01

    Full Text Available The purpose of this study was to compare the reaction of rat subcutaneous connective tissue to 0.9% sterile saline, 2.5% sodium hypochlorite (NaOCl, 5.25% NaOCl and 2% chlorhexidine gluconate solution or gel. Six circles were demarcated on the dorsal skin of 24 male Wistar rats, leaving 2 cm between each circle. Using a syringe, 0.1 mL of each root canal irrigant was injected subcutaneously into 5 circles. In the 6th circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group. Evaluations were undertaken at 2 h, 48 h, 14 days and 30 days post-procedure. Tissue samples were excised, embedded in paraffin blocks and 3-µm-thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated and analyzed statistically by ANOVA and Tukey's test. The control group showed few or no inflammatory reaction areas in the subcutaneous tissue. 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl showed a good biocompatibility, as very mild inflammatory reaction was detected at 14 days and tissue repair occurred at 30 days. 5.25% NaOCl was the most toxic irrigant, as the number of inflammatory cells remained elevated at 14 and 30 days. The group treated with 2.0% chlorhexidine gluconate gel presented a moderate inflammatory response at 14 days, which decreased at 30 days, being considered similar to that of the control group, 0.9% saline solution, 2.0% chlorhexidine solution and 2.5% NaOCl at this experimental period.

  19. Synthesis of biocompatible nanoparticle drug complexes for inhibition of mycobacteria

    International Nuclear Information System (INIS)

    Tuberculosis (TB) is one of the most critical infectious diseases affecting the world today. Current TB treatment involves six months long daily administration of four oral doses of antibiotics. Due to severe side effects and the long treatment, a patient's adherence is low and this results in relapse of symptoms causing an alarming increase in the prevalence of multi-drug resistant (MDR) TB. Hence, it is imperative to develop a new drug delivery technology wherein these effects can be reduced. Rifampicin (RIF) is one of the widely used anti-tubercular drugs (ATD). The present study discusses the development of biocompatible nanoparticle–RIF complexes with superior inhibitory activity against both Mycobacterium smegmatis (M. smegmatis) and Mycobacterium tuberculosis (M. tuberculosis). Iron oxide nanoparticles (NPs) synthesized by gas phase condensation and NP-RIF complexes were tested against M. smegmatis SN2 strain as well as M. tuberculosis H37Rv laboratory strain. These complexes showed significantly better inhibition of M. smegmatis SN2 strain at a much lower effective concentration (27.5 μg ml−1) as compared to neat RIF (125 μg ml−1). Similarly M. tuberculosis H37Rv laboratory strain was susceptible to both nanoparticle–RIF complex and neat RIF at a minimum inhibitory concentration of 0.22 and 1 μg ml−1, respectively. Further studies are underway to determine the efficacy of NPs–RIF complexes in clinical isolates of M. tuberculosis as well as MDR isolates. (paper)

  20. Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model

    Science.gov (United States)

    Sun, Kwang-Hsiao; Liu, Zhao; Liu, Changjian; Yu, Tong; Shang, Tao; Huang, Chen; Zhou, Min; Liu, Cheng; Ran, Feng; Li, Yun; Shi, Yi; Pan, Lijia

    2016-04-01

    Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.