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Sample records for biochemical parameters linked

  1. Biological dosimetry: biochemical and cellular parameters

    International Nuclear Information System (INIS)

    Early after the beginning of radiobiology studies, biochemistry has led to research of a biological dosimeter. From an extensive literature review, methods were selected that might be suitable for dose assessment via biochemical indicators. By now, research both in laboratory animals and in therapeutic or accidental human exposures, do not allow to retain a biochemical parameter alone for the purpose of diagnosis or prognosis. Several enzymatic activities have been precociously studied after irradiation: from these studies, it seems that analysis of four enzymatic activities in serum (serum glutamic oxaloacetic transaminase, amylase, lactic dehydrogenase, alkaline phosphatase) could be the most useful dosimetry system for mass sorting. Detection of DNA damage or methods for measuring somatic mutations are currently advancing and provide important new opportunities for biological dosimetry of low doses

  2. Parâmetros bioquímicos foliares das espécies Licania tomentosa (Benth. e Bauhinia forficata (Link. para avaliação da qualidade do ar Foliar analyses of biochemical parameters of Licania tomentosa (Benth. and Bauhinia forficata (Link. species for air quality assessment

    Directory of Open Access Journals (Sweden)

    Otávio Luiz Gusso Maioli

    2008-01-01

    Full Text Available Foliar analysis of biochemical parameters were carried out in order to investigate the influence of air pollutants on two tropical tree species (Licania tomentosa (Benth. and Bauhinia forfícata (Link.. Special attention was given to tropospheric ozone due to the fact that concentration levels in the region were found to be up to 140 µg m-3 for a 4 h average time, which is well above the value that can cause injuries to orchides and tobacco (59 µg m-3. Other pollutants such as nitrogen and sulphur oxides were measured and their ambient concentrations were also associated to biochemical alterations in the investigated species.

  3. Accelerated maximum likelihood parameter estimation for stochastic biochemical systems

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    Daigle Bernie J

    2012-05-01

    Full Text Available Abstract Background A prerequisite for the mechanistic simulation of a biochemical system is detailed knowledge of its kinetic parameters. Despite recent experimental advances, the estimation of unknown parameter values from observed data is still a bottleneck for obtaining accurate simulation results. Many methods exist for parameter estimation in deterministic biochemical systems; methods for discrete stochastic systems are less well developed. Given the probabilistic nature of stochastic biochemical models, a natural approach is to choose parameter values that maximize the probability of the observed data with respect to the unknown parameters, a.k.a. the maximum likelihood parameter estimates (MLEs. MLE computation for all but the simplest models requires the simulation of many system trajectories that are consistent with experimental data. For models with unknown parameters, this presents a computational challenge, as the generation of consistent trajectories can be an extremely rare occurrence. Results We have developed Monte Carlo Expectation-Maximization with Modified Cross-Entropy Method (MCEM2: an accelerated method for calculating MLEs that combines advances in rare event simulation with a computationally efficient version of the Monte Carlo expectation-maximization (MCEM algorithm. Our method requires no prior knowledge regarding parameter values, and it automatically provides a multivariate parameter uncertainty estimate. We applied the method to five stochastic systems of increasing complexity, progressing from an analytically tractable pure-birth model to a computationally demanding model of yeast-polarization. Our results demonstrate that MCEM2 substantially accelerates MLE computation on all tested models when compared to a stand-alone version of MCEM. Additionally, we show how our method identifies parameter values for certain classes of models more accurately than two recently proposed computationally efficient methods

  4. Carbamates toxicity in farmers and its assesment through biochemical parameters

    International Nuclear Information System (INIS)

    Prevalent environmental toxicity of various chemical group of pesticides and their effects leading towards increasing morbidity and mortality in the farmers is of great concerned. In this situation the biochemical biomarkers are regarded as meaningful tools for monitoring toxic end points. This work was aimed to assess the toxic impacts of carbamates through some biochemical parameters and useful validity of these biomarkers was also observed. Present results reveal inhibition of cholinesterase activity by 46% whereas bilirubin, urea and creatinine levels in serum were increased and sugar values was decreased at highly significant level (p<0.001). Urine urobilinogen concentration found raised significantly at high level (p<0.001) while protein, urea creatinine and sugar values in urine of the farmers seen non-significant. This study concluded that the selected biochemical parameters can be used as biomarkers to assess the significant toxic effects in the exposed populations. (author)

  5. Effect of Different Psychoactive Substances on Serum Biochemical Parameters

    Science.gov (United States)

    Sanli, Dilek Beker; Bilici, Rabia; Suner, Ozgur; Citak, Serhat; Kartkaya, Kazim; Mutlu, Fezan Sahin

    2015-01-01

    Background: Psychoactive substances affect mainly central nervous system and brain function causing changes in behavior. Objectives: The purpose of this study was to determine the effects of different psychoactive substances on serum biochemical parameters. Patients and Methods: The study included 324 drug dependents, and 69 controls. The patient group was determined according to DSM-IV (The diagnostic and statistical manual of mental disorders, fourth edition) criteria. All patients and control subjects were tested for routine biochemical parameters and urine toxicology parameters for psychoactive substance use. Cases and controls with accompanying diseases like diabetes, cancer, metabolic disorders etc. are excluded from the study. Moreover, an association between urine toxicology results and changes in biochemical parameters was evaluated for statistical significance. Results: There was a statistically significant difference in the Gamma-Glutamyl Transferase (GGT), uric acid, creatinine, urea, albumin, Aspartate Aminotransferase (AST) medians between the dependent and control groups (P < 0.05). We found a statistically significant difference in sodium and albumin levels between the opium-dependent and control groups (P < 0.05). In the benzodiazepin dependent group, we found a significant difference in GGT, urea, glucose, sodium, T protein, and AST levels (P < 0.05). Moreover, a statistically significant difference was observed in triglyceride and GGT levels between the ethyl glucuronide and control groups (P < 0.05). Conclusions: In psychoactive substance dependents, serum routine biochemistry parameters can be used to predict the need for intensive monitoring and treatment programs. PMID:26405680

  6. Prognostic Significance of Various Biochemical Parameters in Acute Organophosphorus Poisoning

    OpenAIRE

    Sumathi, M. E.; Kumar, S. Harish; Shashidhar, K N; Takkalaki, Nandini

    2014-01-01

    Background: Organophosphorus (OP) compounds are a heterogeneous group of insecticides widely used in agricultural industry. These OP compounds are likely to have more adverse effects in developing countries like India due to its easy availability and less awareness which results in high morbidity and mortality. Aims and objectives: 1. To estimate plasma cholinesterase, amylase, lipase and, creatine phosphokinase (CPK) in acute OP poisoning. 2. To correlate these biochemical parameters with pl...

  7. Clinical and Biochemical Parameters of Children and Adolescents Applying Pesticides

    OpenAIRE

    O Hendy; M Abou Salem; G Abdel Rasoul; D Rohlman; A. Ismail

    2010-01-01

    Background: The primary agricultural product in Egypt is the cotton crop. Children and adolescents work seasonally in the cotton fields applying pesticides.Objectives: To examine the effect of pesticide exposure on clinical and biochemical parameters in children and adolescents applying pesticides.Methods: Male children currently applying pesticides and aged between 9 and 19 years (n = 50) were recruited for this study. They were asked to complete work, health, and exposure questionnaires; ex...

  8. Studies on some biochemical parameters in viral hepatitis patients

    International Nuclear Information System (INIS)

    The present investigation deals with studying liver amino transferases (ALT. AST). Cholesterol and triglycerides. As well as testosterone and protection hormones in blood of Egyptian men infected with hepatitis C virus.hepatitis B virus and mixed B and C viruses. These biochemical parameters were evaluated to be used in diagnosis and prognosis of viral hepatitis. Which considered the most important health problem in Egypt and developing countries. Biochemical analysis were performed using spectrophotometric and radioimmunoassay techniques. All data will be subjected to statistical analysis in order to detect the most suitable biochemical analysis that can be used as specific tests for early diagnosis of viral hepatitis and to detect the parameters that show abnormalities among the different groups of infected patients. The data revealed that AST and ALT levels were increased in all patient groups. Concerning the level of triglycerides, it was increased only in the group of mixed viral hepatitis B and C, while cholesterol showed non-significant changes in all viral hepatitis groups. The sex hormone testosterone was decreased in all infected patients while the prolactin level was increased only in case of patients infected with mixed B and C viruses. However, these abnormal values in such sex hormones play a serious role in male sterility

  9. Gadagi Magani Tea Is Detrimental Affects Biochemical Parameters

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    Umar A.A.

    2015-08-01

    Full Text Available Abstract Gadagi Magani tae is a herbal tea composed of many different herbs roots extracts shrubs and leaves prepared locally in form of tea consumed by many labourers youths tailors tricyclists and high way drivers in order to get extraordinary strength to endure hardship and overcome heavy duty within short period of time. The following biochemical parameters were routinely determined among consumers serum level of Aspartate aminotransaminases AST Alanine aminotransferase ALT activities were pragmatically higher than that of control increase in serum creatinine level lower sera urea and glucose levels and the sera levels of lipoproteins were also lower compared to controls. Hence Gadagi affects negatively the liver enzymes blood sugar level serum urea creatinine and carbonate positively as it lowers lipid profile.

  10. Effect of nitrate poisoning on some biochemical parameters in rats

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    M. B. Mahmood

    2011-01-01

    Full Text Available The present study was conducted to investigate the toxicity of potassium nitrate on glucose, cholesterol, alanine aminotransferase (ALT, aspartate aminotransferase (AST, and the possible ameliorative effect of ascorbic acid (Vitamin C. Male Wister rats are used as experimental model divided into three groups (each of 6-8 rats and treated for six weeks as follows: Group 1: served as control; Group 2: received 2 % potassium nitrate added to the forage and Group 3: received 2 % potassium nitrate together with 1 % ascorbic acid added to rat's forage. Nitrate treatment in group 2 leads to high significant increase levels of glucose in 3rd, 4th, and 5th weeks, cholesterol level increased significantly in both 4th and 5th weeks, while ALT levels increased in the 4th, 5th and 6th weeks, and AST increased significantly in the 5th and 6th weeks. Addition of ascorbic acid with potassium nitrate, lead to reverse all the parameters nearly to normal. It was concluded that potassium nitrate causes significant toxic effect on some biochemical parameters which was ameliorated by ascorbic acid.

  11. Clinical and Biochemical Parameters of Children and Adolescents Applying Pesticides

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    O Hendy

    2010-06-01

    Full Text Available Background: The primary agricultural product in Egypt is the cotton crop. Children and adolescents work seasonally in the cotton fields applying pesticides.Objectives: To examine the effect of pesticide exposure on clinical and biochemical parameters in children and adolescents applying pesticides.Methods: Male children currently applying pesticides and aged between 9 and 19 years (n = 50 were recruited for this study. They were asked to complete work, health, and exposure questionnaires; examined for any medical and neurological problems with particular attention to sensory and motor functions including cranial nerves, sensory and motor system, and reflexes. From each participant, a blood sample was taken to measure acetylcholinesterase activity, and liver and kidney functions. Children who have never worked in agriculture (n = 50, matched on age, education, and socioeconomic status were also studied and served as controls.Results: More neuromuscular disorders were identified in pesticide applicators than controls. A significant lower level of acetylcholinesterase was found in the applicator group compared to the controls. There was also a significant difference in hematological, renal and hepatic indices in the exposed children compared to the control children. Working more days in the current season and also working more years as a pesticide applicator were both associated with an increase in the prevalence of neuromuscular abnormalities and significant changes in the laboratory tests.Conclusion: Children and adolescent pesticide applicators working in farms of Egypt are at risk of developing serious health problems similar to those of adults.

  12. THE STUDY OF SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH MULTIPLE MYELOMA

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    Bularda Morozan Mihai

    2010-09-01

    Full Text Available This paper presents the results of some biochemical parameters obtained in the laboratory of “Elena Beldiman” Emergency Hospital Barlad, and in the”Dr. Stoian –Dr. Ungureanu”Private Medical Practice on 10 patients diagnosed with multiple myeloma during 2005-2011. Numerous researches over serum proteins with the help of various methods ( refractometry have shown the presence of some changes in protein fractions of blood serum. These changes express the change of the normal ratio between these fractions, that means disorders in colloidal structure of blood. The results of the electrophoresis analysis, also in accordance with those from the scholarly literature, show that the changes in the condition of the blood serum are due to the increase in the globulins fractions which is linked to the growth of the immunological processes, the antibodies synthesis being especially closely linked to gammaglobulinic fraction

  13. A feedback approach to bifurcation analysis in biochemical networks with many parameters

    OpenAIRE

    Waldherr, Steffen; Allgower, Frank

    2007-01-01

    Feedback circuits in biochemical networks which underly cellular signaling pathways are important elements in creating complex behavior. A specific aspect thereof is how stability of equilibrium points depends on model parameters. For biochemical networks, which are modelled using many parameters, it is typically very difficult to estimate the influence of parameters on stability. Finding parameters which result in a change in stability is a key step for a meaningful bifurcation analysis. We ...

  14. Achievable qualitative parameters of optical wireless links

    Czech Academy of Sciences Publication Activity Database

    Kolka, Z.; Wilfert, O.; Fišer, Ondřej

    2007-01-01

    Roč. 9, č. 8 (2007), s. 2419-2423. ISSN 1454-4164 R&D Projects: GA ČR(CZ) GA102/05/0571 Institutional research plan: CEZ:AV0Z30420517 Keywords : Free-space optical link * Transmission of atmosphere * Optical communication Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.827, year: 2007 http://inoe.inoe.ro/joam/index.php?option=magazine&op=view&idu=838&catid=16

  15. Hyperhomocysteinemia: a biochemical link between bone and cardiovascular system diseases?

    Science.gov (United States)

    Petramala, L; Acca, M; Francucci, C M; D'Erasmo, E

    2009-01-01

    Homocysteine (HCY) is a sulfur-containing amino acid involved in two metabolic pathways, catalized by cystathionine-B-synthase and methionine synthase, depending on vitamin (vit) B6, B12, and folate levels and enzymatic activity of methylenetetrahydrofolate. High HCY levels (HHCY) are associated with cardiovascular (CV) and bone diseases, in particular osteoporosis (OP)/hip fracture. As regards the mechanisms involved in the link between HHCY, CV diseases (CVD), and OP, it has been proposed the role of lysyl-oxydase inhibition that might interfere with collagen crosslink formation. Some studies suggested the dysregulation of the osteoprotegerin/receptor activator of nuclear factor-kappaB (RANK) ligand/RANK axis, others the involvement of oxidative stress. These mechanisms may act both on bone and CV system, but whether the common denominator is HCY itself or HCY is merely a marker, remains to be clearly established. Folate, vit B6, and B12 supplementation is associated with HCY reduction, but is unable to certainly reduce the incidence of OP/fracture and CVD, probably because, in the majority of patients, HCY is only moderately increased. PMID:19724160

  16. A new statistical biomechanics modeling of physical and biochemical parameters of bone strength

    Energy Technology Data Exchange (ETDEWEB)

    Soboyejo, A.B.O. [Ohio State Univ., Columbus, OH (United States). Dept. of Aerospace Engineering, Applied Mechanics and Aviation; Nestor, K.E. [Ohio State Univ., Wooster, OH (United States). Dept. of Animal Sciences

    2001-07-01

    New multiparameter biomechanics models were developed in this work for the characterization of bone strength, as functions of the major physical and biochemical parameters, which can contribute to mechanical properties of bone strength. Theoretical and experimental methods had been developed to model bone strength as functions of (a) the physical parameters and (b) the biochemical parameters, The choice of any particular methodology will depend on the availability of either the physical or biochemical parameters. Experimental data of compressive strength of tibia and femur bones of broiler chickens and turkeys together with their corresponding physical and biochemical parameters were collected and used as examples in this study. These data were used to validate the theoretical principles developed in this work. Useful practical applications of the statistical biomechanics principles developed in this study, particularly in the field of bone strength enhancement in turkeys and broiler chickens are discussed. Similar medical applications to human beings are also highlighted in the discussions. (orig.)

  17. Effects of angiotensin converting enzyme inhibitor: ramipril on different biochemical parameters in essential hypertensive patients

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    Pratibha S. Salve

    2016-06-01

    Conclusions: Ramipril has beneficial effects on RAS (Renin angiotensin system and kinin system or both may contribute to the improvement in different biochemical parameters by ramipril. [Int J Res Med Sci 2016; 4(6.000: 2288-2291

  18. Relationship between daily dietary fructose intake, body composition and biochemical parameters patients with type 2 diabetes

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    Sabriye Arslan

    2016-05-01

    Conclusion: Further studies are needed to develop more specific suggestions regarding fructose intake and to better understand the relationship between fructose intakes, body composition and biochemical parameters in individuals with type 2 diabetes.

  19. Biochemical parameters of the amazonian manatee´s blood (Trichechus inunguis, Mammalia: Sirenia)

    OpenAIRE

    Elton Pinto Colares; Ioni Gonçalves Colares; Ary Domingos do Amaral

    2011-01-01

    The blood biochemical and hematological parameters make possible to acquire information about the physiological situation of animals or their population, permitting in this way the diagnosis of possible pathologies. The objective of this work was to check the normal values of some biochemical parameters of the captive Amazonian manatee´s blood and compare them with other animals´. In this study, 10 Amazonian manatees kept in captivity in the Instituto Nacional de Pesquisas da Amazônia, being ...

  20. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

    OpenAIRE

    Petra Hrabčáková; Eva Voslářová; Iveta Bedáňová; Vladimíra Pištěková; Jan Chloupek; Vladimír Večerek

    2014-01-01

    The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P ...

  1. Global parameter estimation methods for stochastic biochemical systems

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    Poovathingal Suresh

    2010-08-01

    Full Text Available Abstract Background The importance of stochasticity in cellular processes having low number of molecules has resulted in the development of stochastic models such as chemical master equation. As in other modelling frameworks, the accompanying rate constants are important for the end-applications like analyzing system properties (e.g. robustness or predicting the effects of genetic perturbations. Prior knowledge of kinetic constants is usually limited and the model identification routine typically includes parameter estimation from experimental data. Although the subject of parameter estimation is well-established for deterministic models, it is not yet routine for the chemical master equation. In addition, recent advances in measurement technology have made the quantification of genetic substrates possible to single molecular levels. Thus, the purpose of this work is to develop practical and effective methods for estimating kinetic model parameters in the chemical master equation and other stochastic models from single cell and cell population experimental data. Results Three parameter estimation methods are proposed based on the maximum likelihood and density function distance, including probability and cumulative density functions. Since stochastic models such as chemical master equations are typically solved using a Monte Carlo approach in which only a finite number of Monte Carlo realizations are computationally practical, specific considerations are given to account for the effect of finite sampling in the histogram binning of the state density functions. Applications to three practical case studies showed that while maximum likelihood method can effectively handle low replicate measurements, the density function distance methods, particularly the cumulative density function distance estimation, are more robust in estimating the parameters with consistently higher accuracy, even for systems showing multimodality. Conclusions The parameter

  2. Association between Renal Stone, Bone Mineral Density and Biochemical Parameters

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    Zh Maghbooli

    2007-01-01

    Results: Data showed that patients with a history of renal stones had a higher prevalence of osteoporosis (16.7% and os¬teopenia (53.3% than the subjects without a history of renal stone disease (11.2% and 35.7%, respectively. For both men and women the mean age of patients with a history of renal stone disease was significantly lower than patients with no dis¬ease history (men: with history 44.27+/-14.8, without history 50.28+/-12.3; P= 0.02 (women: with history 43.21+/-11.8, with¬out history 49.06+/-9.6; P= 0.02. Female patients with a history of renal stone disease also had a significantly lower (8.74% mean spinal bone density (P= 0.02, but there were no other significant differences in either the biochemical parame¬ters that were measured or in the hip bone density. Conclusions: These data suggest that osteoporosis may be more prevalent in those patients that have had a history of renal stone formation.

  3. Effects of ramadan fasting on cardiovascular and biochemical parameters

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    M Gupta

    2013-12-01

    Full Text Available Background: Muslims abstain from food and drink from dawn to sunset every day during Ramadan - the holiest month in Islamic lunar calendar.  Methods: The effect of Ramadan fast on body mass index (BMI, blood pressure (BP, fasting blood glucose (FBG and lipid profile were studied on 100 healthy male, adult Muslim volunteers. All parameters were recorded one week before the onset and then in the last week of Ramadan month and compared.  Results: There was no statistically significant effect on BMI, and systolic or diastolic blood pressures (p>0.05. However, fasting blood glucose (FBG, serum total cholesterol (TC, serum triglycerides (TG were significantly decreased (p = 0.000 and high density lipoprotein cholesterol (HDL-C level was significantly (p=0.000 increased in last week of Ramadan fasting compared to pre fasting levels. Conclusion: Our results show beneficial effects of Ramadan fasting on FBG and serum lipid profile. 

  4. Composted Rice Husk Improves the Growth and Biochemical Parameters of Sunflower Plants

    OpenAIRE

    Rabia Badar; Qureshi, Shamim A.

    2014-01-01

    The present study investigated the effects of composted rice husk (5 and 26; 10 g/2 kg of soil/pot) on growth and biochemical parameters of sunflower plants at the 30th and 60th day of germination. Result showed significant improvement in growth and biochemical parameters of plants as compared to control plants treated with uncomposted organic fertilizer. However, the effects vary with the microbial treatments involved in the composting of rice husk like composted with T. hamatum (JUF1), brad...

  5. Biochemical Intestinal Parameters in Pigs Reared Outdoors and Indoors and in Germ-free Pigs

    Czech Academy of Sciences Publication Activity Database

    Collinder, E.; Cardona, M.; Kozáková, Hana; Norin, E.; Stern, S.; Midtvedt, T.

    2002-01-01

    Roč. 49, - (2002), s. 1-7. ISSN 0931-184X R&D Projects: GA ČR GA306/99/1383 Grant ostatní: Agriculture(SE) 5899/96; Medical Research Council(SE) 16x-06852 Keywords : biochemical * intestinal * parameters Subject RIV: EE - Microbiology, Virology Impact factor: 0.567, year: 2002

  6. Relationship Between Hyperspectral Parameters and Physiological and Biochemical Indexes of Flue-Cured Tobacco Leaves

    Institute of Scientific and Technical Information of China (English)

    LI Xiang-yang; LIU Guo-shun; YANG Yong-feng; ZHAO Chun-hua; YU Qi-wei; SONG Shi-xu

    2007-01-01

    The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.

  7. Effect of taking dietary supplement on hematological and biochemical parameters in male bodybuilders an equation model

    OpenAIRE

    Meamar, Rokhsareh; Maracy, Mohammad; Nematollahi, Shahrzad; Yeroshalmi, Shemouil; Zamani-Moghaddam, Ali; Ghazvini, Mohammad Reza Aghaye

    2015-01-01

    Background: The improved physical action following administration of supplements to bodybuilders was supported by changes in laboratory parameters. Despite the fact that these supplements are sometimes associated both advantage and side effects, this study were conducted for the purpose of evaluating the possible effects of some commonly used supplements in bodybuilders on the hematological and biochemical parameters. Materials and Methods: In this study, we included 40 male bodybuilders as c...

  8. Correlation between yield and biochemical parameters in the mulberry silkworm,Bombyx mori L.

    Science.gov (United States)

    Chatterjee, S N; Rao, C G; Chatterjee, G K; Ashwath, S K; Patnaik, A K

    1993-11-01

    A detailed study was carried out on six biochemical parameters and four yield attributes using multiple regression analysis to investigate their relationship in the mulberry silkworm,Bombyx mori. The study generated new information on the importance of digestive amylase activity for the survival of the silkworm and revealed the inability of other enzymes to affect this relationship. Data also substantiate the observations made earlier on the genetic variability of amylase in the mulberry silkworm. Analyses extend the positive role of alkaline phosphatase and invertase in the expression of the other yield traits studied and indicate the definite possibility of using biochemical markers for silkworm breeding. PMID:24190267

  9. Association of serum fetuin-A and biochemical parameters in hemodialysis patients

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    Mahbobeh Haddad

    2014-01-01

    Full Text Available Fetuin-A, a hepatic glycoprotein present in the circulation, is a potential inhibitor for systemic calcification. The main aim of this study was to evaluate the association between fetuin-A and other biochemical parameters as facilitator factors for developing atherosclerosis in hemodialysis (HD patients. This case-control study was conducted on 44 HD patients undergoing treatment in 2012. Parathormone (i-PTH and fetuin levels were performed by the enzyme-linked immunosorbent assay method, high-sensitivity C-reactive protein (hs-CRP by chemiluminescence, low-density lipoprotein by direct enzymatic, calcium and albumin by colorimetric and phosphorous by ultraviolet (UV methods. Chi-square was used for evaluating the association between variables and t-test was used for comparing the mean of the quantitative variables for the two groups. SPSS-16 software was used for data analysis and P-value less than 5% was considered as significant. Mean of serum fetuin level was 23.25 ± 4.90 ng/mL in HD patients and 32.92 ± 5.21 in the control group. Median of hs-CRP was 2.45 mg/dL in the patients and 1.00 mg/dL in the control group and i-PTH was 74.3 pg/mL in the patients and 7.30 pg/mL in the control group. The calcium-phosphorous product was 46.77 ± 14.22 mg/dL in the patient and 31.73 ± 6.48 mg/dL in the control group. A reverse significant association was found between fetuin-A and hs-CRP in this study. In this study, serum fetuin-A level in HD patients was lower than controls. Therefore, a low level of fetuin-A seems to be associated with atherosclerosis, inflammation and malnutrition.

  10. Parameters Obtained by Hepatobiliary Scintigraphy Have Significant Correlation with Biochemical Factors Early After Liver Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Kansoul, H.A.; Axelsson, R.; Yamamoto, S.; Savicheva, I.; Aspelin, P.; Ericzon, B.G.; Gjertsen, H. [Div. of Transplantation Surgery and Div. of Radiology, Dept. of Clinical Science, Intervention, and Technology, Karolinska Inst., Karolinska Univ. Hospital, Stockholm (Sweden)

    2007-08-15

    Background: Early postoperative hepatobiliary scintigraphy after liver transplantation is performed worldwide, but data on its significance for graft function are currently limited. Purpose: To examine the correlation between the result of early postoperative hepatobiliary scintigraphy and pre- and postoperative biochemical parameters in liver transplantation (LTx) patients. Material and Methods: Six parameters of hepatobiliary scintigraphy using {sup 99m}Tc mebrofenin were statistically analyzed in 108 LTx patients: 1) half-life of the activity of elimination of mebrofenin from the blood; 2) total clearance of mebrofenin from the blood due to all possible routes; 3) half-life of the activity due to liver uptake; 4) clearance of mebrofenin from the blood due to liver uptake; 5) time to maximal uptake in the liver; and 6) the hepatic extraction fraction (HEF) and biochemical data. Analysis between patients with preoperative normal liver function, familial amyloid polyneuropathy (FAP), and end-stage liver disease (non-FAP) was also performed. Results: Univariate and multivariate analysis revealed that total bilirubin postoperative day 3 correlated with all three scintigraphic parameters, and peak aspartate aminotransferase and alanine aminotransferase correlated with HEF. The analysis between patients with FAP and non-FAP revealed no significant difference of scintigraphic data between the two groups. Conclusion: A significant correlation between early postoperative scintigraphic results and biochemical parameters was demonstrated.

  11. Correlation between Aminotransferase Ratio (AST/ALT and Other Biochemical Parameters in Chronic Liver Disease of Viral Origin

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    Shah Md Fazlul Karim

    2015-03-01

    Full Text Available Background: In recent years the ratio of aspartate aminotransferase (AST to alanine aminotransferase (ALT in patients of chronic liver disease (CLD of various origins has gained much attention. This variable is readily available, easy to interpret, and inexpensive and the clinical utility of the AST/ALT ratio in the diagnostic workup of patients with CLD is quite promising. Objective: The present study was designed to find out the link between aminotransferase (AST/ALT ratio with commonly measured biochemical parameters of liver function tests in CLD of viral origin. Materials and method: This cross sectional study was carried out in the department of Biochemistry, Sir Salimullah Medical College, Dhaka, Bangladesh. Forty four biopsy proven diagnosed subjects of chronic viral hepatitis without cirrhosis of both sex were selected purposively. With aseptic precaution 5 mL venous blood was collected from each subject and common liver function tests (serum AST, ALT, AST/ALT ratio, alkaline phosphatase, total bilirubin, serum total protein, serum albumin, serum globulin, serum albumin/globulin ratio, prothrombin time and viral serology (HBsAg, Anti HDV antibody, Anti HCV antibody were performed. Data were analyzed by SPSS version 19 for Windows. Pearson’s correlation test was done to determine association between AST/ALT with other biochemical parameters. Results: Mean(±SD age of the study subjects was 32.55±10.55 years (range 20-50 years with 48 (77.7% male and 14 (22.6% female subjects. Pearson’s correlation test was done between AST to ALT ratio with other biochemical parameters and prothrombin time showed significant positive correlation (p <0.01. Conclusion: In our study we found significant positive correlation between AST/ALT with prothrombin time in CLD subjects without cirrhosis.

  12. Kilimanjaro Abruzzo expedition: effects of high-altitude trekking on anthropometric, cardiovascular and blood biochemical parameters

    OpenAIRE

    Verratti, Vittore; Falone, S.; Doria, C.; Pietrangelo, T.; Di Giulio, C.

    2015-01-01

    The effect of the combination of trekking and balanced appropriated diet were studied in mountaineers who spent 6 days at an altitude ranging from 900 to 5895 m above sea level (a.s.l.), during the Kilimanjaro Abruzzo Expedition. This study explored whether anthropometric, cardiovascular and blood biochemical parameters were significantly changed by a regular trekking performed at high altitude, with reduced oxygen levels, together with a macronutrient-containing balanced diet (total daily ca...

  13. Effect of ferrous sulphate on haematological, biochemical and immunological parameters in neonatal calves

    OpenAIRE

    Lobna S. Elgebaly; Adel M.A. Eisa

    2010-01-01

    The effect of oral administration of iron on haematological, biochemical and immunological parameters in neonatal calves was studied. Ten calves from a private farm in Gharbia Governorate were used. Calves were separated from their dams immediately after birth and received colostrum during the first hours after calving and twice daily for 48 h. Thereafter, they received whole milk. Calves were divided into two equal groups. The first group was kept as controls. Calves of the second group were...

  14. Effects of Fermented Soybean Meal on Performance, Serum Biochemical Parameters and Intestinal Morphology of Laying Hens

    OpenAIRE

    X.L. Ding; Wu, D.; Qian, K.; K. Zhan; Liu, H. J.; L.M. Li; Xu, F. Z.

    2012-01-01

    This experiment was performed to compare the effects of fermented soybean meal (fermented with Bacillus licheniformis D-1, FSBM) and Soybean Meal (SBM) on performance, serum biochemical parameters and intestinal morphology of laying hens. About 288, 29 weeks old laying hens were randomly allocated into 2 dietary treatments, 4 replicate groups of 36 laying hens each from 29-39 weeks of age. One treatment received the basal diet (containing 22.8% SBM) as control and the other treatment received...

  15. The effects of Ramadan fasting and physical activity on body composition and hematological biochemical parameters

    OpenAIRE

    Seyyed Reza Attarzadeh Hosseini; Morteza Motahari Rad; Keyvan Hejazi

    2014-01-01

    Introduction: Hunger and reduction in regular energy intake can lead to a number of problems based on their intensity. For instance, low energy level can cause blood cell production to decline or it may pose a higher risk of anemia. It can also weaken the immune system and platelet aggregation or negatively affect clot formation. This study aimed to have a closer look at fasting and regular physical activity and their impacts on body composition and blood hematological-biochemical parameters ...

  16. Effect of toxoplasmosis and brucellosis on some biochemical parameters in ewes

    OpenAIRE

    N. A. J. Al- Hussary; A. S. M. Al- Zuhairy

    2010-01-01

    The present study was conducted to evaluate the effects of infection of ewes with toxoplasmosis and/or brucellosis on someserum biochemical parameters. Ninety six samples of blood were collected from aborted ewes at different stages of gestation,suspected to be infected with toxoplasmosis and /or brucellosis from different regions in Nineveh governorate. The percentageof toxoplasma and brucella infection depending on Latex Agglutination Test (LAT) and Rose Bengal Test were 21.88% and23.96% re...

  17. Impact of sudarshan kriya yoga on mean arterial blood pressure and biochemical parameters in medical students

    Directory of Open Access Journals (Sweden)

    Nisar Ahmad

    2016-06-01

    Conclusions: Living a happy and healthy life on all planes is possible through the unified practice of sudarshan kriya yoga (SKY along with asana and pranayam. The practice of SKY seems to be beneficial on mean blood pressure and biochemical parameters in normal healthy individuals. This unique breathing practice is a potent energizer. [Int J Res Med Sci 2016; 4(6.000: 2150-2152

  18. Women with metabolic syndrome improve antrophometric and biochemical parameters with green banana flour consumption

    OpenAIRE

    Sandra Tavares da Silva; Carolina Araujo dos Santos; Yassana Marvila Girondoli; Luiza Mello de Azeredo; Luis Fernando de Sousa Moraes; Josiane Keila Viana Gomes Schitini; Mario Flävio C. de Lima; Raquel Cristina Lopes Assis Coelho; Josefina Bressan

    2014-01-01

    Background: Consumption of green banana flour (GBF) may promote health benefits, such as, decreased appetite, weight loss, glycemic control, intestinal function and lipid profile improvement, aging delay, cancer and heart disease prevention. The aim of this study was to evaluate the effects of green banana flour consumption on anthropometric and biochemical parameters in overweight women. Methods: The glycemic index of flour in the study was determined. The effects of consumption of 20 g of g...

  19. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

    OpenAIRE

    Fabrícia de Souza Predes; Sérgio Luis Pinto da Matta; Juliana Castro Monteiro; Tânia Toledo de Oliveira

    2009-01-01

    This study was carried out to evaluate the effects of Arctium lappa L. (burdock) on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT), total protein, total cholesterol, urea, uric acid, triacylglycerol,...

  20. Investigation of some hematological and blood biochemical parameters in cattle spontaneously infected with bovine leukosis virus

    OpenAIRE

    Sandev Nikolay; Zapryanova Dimitrinka; Stoycheva Ivanka; Rusenova Nikolina; Mircheva Teodora

    2013-01-01

    The aim of the present study was to follow out the alterations in some haematological and blood biochemical parameters in cattle spontaneously infected with enzootic bovine leukosis virus with regard to the invivodifferentiation of bovine leukosis stages. The experiment included 76 cows at various ages and body weight. Serological leukosis tests were done by agar-gel immunodiffusion test with a commercial kit of Synbiotiсs (France), containing standardised gp 51 antigen and positive serum app...

  1. The effects of adenosine on plasma homocysteine levels and some other biochemical parameters

    OpenAIRE

    Turgut, Günfer; Rota, Simin; Aybek, Hülya; Turgut, Sebahat; Sert, Seelahattin; Genç, Osman

    2009-01-01

    Süleyman Demirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2007 Aralık; 14(4) The effects of adenosine on plasma homocysteine levels and some other biochemical parameters Günfer Turgut*, Simin Rota**, Hülya Aybek**, Sebahat Turgut*, Selahattin Sert**, Osman Genç* *Pamukkale University Faculty of Medicine Departments of Physiology, Denizli, Turkey **Pamukkale University,Faculty of Medicine, Departments of Biochemistry Denizl...

  2. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

    OpenAIRE

    Prokić Marko D.; Paunović Milica G.; Matić Miloš M.; Đorđević Nataša Z.; Ognjanović Branka I.; Štajn Andraš Š.; Saičić Zorica S.

    2015-01-01

    Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased i...

  3. Effects of Race Distance on Physical, Hematological and Biochemical Parameters of Endurance Horses

    OpenAIRE

    Lawan Adamu; Noraniza M. Adzahan; Rasedee Abdullah; Bashir Ahmad

    2010-01-01

    Problem statement: Endurance horses are generally exposed to severe stress during endurance competitions. These horses are however conditioned to cover the long distances of the competitions at moderate speeds. Approach: This study was conducted in Malaysia to determine the post-race hematological, blood electrolyte and biochemical and physical parameters of horses of 40, 80 and 120 km endurance race categories and eliminated and horses that completed the races with good&#...

  4. Impact of environmental stress on biochemical parameters of bacteria reducing chromium

    Directory of Open Access Journals (Sweden)

    Rida Batool

    2014-06-01

    Full Text Available Chromium pollution is produced in connection with industrial processes like in tanneries. It has been suggested that bioremediation could be a good option for clean up. The stress effect of variable chromate levels, pHs and growth temperatures on biochemical parameters of two Cr(VI reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 was investigated. Transmission electrone microscopy (TEM was performed to study the intracellular distribution of Cr(VI. It was observed that initial stress of 1000 µgmL-1 caused significant enhancement of all studied biochemical parameters at pH 7.0 and growth temperature of 37 °C showing great bioremediation potential of the strains. Transmission electron microscopy revealed that the distribution of chromium precipitates was not uniform as they were distributed in the cytoplasm as well as found associated with the periplasm and outer membrane. Fourier transform infrared spectroscopy showed the possible involvement of carboxyl, amino, sulpohonate and hydroxyl groups present on the bacterial cell surface for the binding of Cr(VI ions. Cr(VI stress brought about changes in the distridution of these functional groups. It can be concluded that the investigated bacterial strains adjust well to Cr(VI stress in terms of biochemical parameters and along that exhibited alteration in morphology.

  5. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

    Directory of Open Access Journals (Sweden)

    Petra Hrabčáková

    2014-01-01

    Full Text Available The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P<0.05 in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P<0.05. At the end of the laying period, an increase (P<0.05 was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P<0.05 were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.

  6. Predictive value of routine hematological and biochemical parameters on 30-day fatality in acute stroke

    Directory of Open Access Journals (Sweden)

    Bhatia R

    2004-04-01

    Full Text Available OBJECTIVE: This prospective study was planned to study the prognostic value of routine clinical, hematological and biochemical parameters, including platelet aggregation in patients of acute stroke, on fatality occurring during the first 30 days. MATERIAL AND METHODS: In this study 116 consecutive patients (77 males and 39 females of stroke (within 72 hours of onset were included. After clinical evaluation and neuroimaging, blood investigations, hemoglobin, total leukocyte count, platelet count, platelet aggregation, erythrocyte sedimentation rate (ESR, blood sugar, urea, creatinine, sodium, potassium, serum cholesterol, serum bilirubin, aspartate aminotransferase (SGOT, alanine aminotransferase (SGPT, albumin, and globulin estimations were performed. The patients were followed up for a maximum period of 30 days from the onset of stroke, and patients who expired were grouped as ′expired′ and the rest as ′survivors′. Logistic regression analysis was carried out among the significant parameters to identify independent predictors of 30-day fatality. RESULTS: Univariate analysis demonstrated that among hematological parameters, high total leukocyte count and ESR, at admission, correlated significantly with an undesirable outcome during the initial 30 days. Among biochemical parameters, elevated urea, creatinine, serum transaminases (SGOT and SGPT and globulin levels correlated significantly with death. Logistic regression analysis demonstrated that a low Glasgow Coma Scale (GCS score along with biochemical parameters such as high serum creatinine, SGPT, ESR and total leukocyte count correlated with death. CONCLUSION: Impaired consciousness, high total leukocyte count, raised ESR, elevated creatinine and SGPT levels, estimated within 24 hours of hospitalization, are the most important indicators of 30-day mortality in patients with first-time ischemic stroke.

  7. Effect of toxoplasmosis and brucellosis on some biochemical parameters in ewes

    Directory of Open Access Journals (Sweden)

    N. A. J. Al- Hussary

    2010-01-01

    Full Text Available The present study was conducted to evaluate the effects of infection of ewes with toxoplasmosis and/or brucellosis on someserum biochemical parameters. Ninety six samples of blood were collected from aborted ewes at different stages of gestation,suspected to be infected with toxoplasmosis and /or brucellosis from different regions in Nineveh governorate. The percentageof toxoplasma and brucella infection depending on Latex Agglutination Test (LAT and Rose Bengal Test were 21.88% and23.96% respectively. The results of biochemical analysis showed that infection with toxoplasma caused significant elevation ofserum activities of aspartate aminotransferase (AST, alanine aminotransferase (ALT and lactate dehydrogenase (LDH. Aswell as the concentrations of glucose, total protein and copper. Where as the activity of alkaline phosphatase (ALP and theconcentration of calcium and zinc were reduced. Infection with brucellosis caused a significant increased in serum AST, ALTand LDH, ALP and creatin phosphokinase (CPK activities, and glucose, zinc and cholesterol concentrations. While serumcalcium and total protein concentration were decreased. Association of toxoplasma and brucella infection induced significantelevation of serum AST, ALT and LDH and CPK activities and the concentrations of glucose and copper with significant reduction in serum ALP activity and both calcium and zinc concentrations. It was concluded from this study that infection ofewes with toxoplasma and/or brucella caused changes in some biochemical parameters in the serum.

  8. Bayesian inference of biochemical kinetic parameters using the linear noise approximation

    Directory of Open Access Journals (Sweden)

    Finkenstädt Bärbel

    2009-10-01

    Full Text Available Abstract Background Fluorescent and luminescent gene reporters allow us to dynamically quantify changes in molecular species concentration over time on the single cell level. The mathematical modeling of their interaction through multivariate dynamical models requires the deveopment of effective statistical methods to calibrate such models against available data. Given the prevalence of stochasticity and noise in biochemical systems inference for stochastic models is of special interest. In this paper we present a simple and computationally efficient algorithm for the estimation of biochemical kinetic parameters from gene reporter data. Results We use the linear noise approximation to model biochemical reactions through a stochastic dynamic model which essentially approximates a diffusion model by an ordinary differential equation model with an appropriately defined noise process. An explicit formula for the likelihood function can be derived allowing for computationally efficient parameter estimation. The proposed algorithm is embedded in a Bayesian framework and inference is performed using Markov chain Monte Carlo. Conclusion The major advantage of the method is that in contrast to the more established diffusion approximation based methods the computationally costly methods of data augmentation are not necessary. Our approach also allows for unobserved variables and measurement error. The application of the method to both simulated and experimental data shows that the proposed methodology provides a useful alternative to diffusion approximation based methods.

  9. Amelioration of some biochemical parameters in irradiated male albino rats by garlic

    International Nuclear Information System (INIS)

    Garlic extract has various medical effects on the treatment of many diseases as hypertension, atherosclerosis, inflammation and diabetes. The alteration of the biochemical parameters in blood serum of irradiated rats may play an important role in determining the pathogenesis of radiation exposure. Many of the damaging effects of ionizing radiation are mediated by reactive free radicals. This study was designed to evaluate the protective role of garlic against gamma irradiation (5Gy) induced biochemical disorders in rats. Samples were collected at 1, 7 and 14 days post-irradiation. Lipid peroxide content (malondialdehyde), cholesterol, HDL-C, LDL-C, fatty acids, glucose, insulin, glycogen, haemoglobin, ferritin andiron were estimated.Garlic was orally administered to rats (100 mg/kg body weight) for 14 days before exposure to single dose of gamma irradiation at dose level 5 Gy. The data revealed significant increase in the levels of lipid peroxide, cholesterol, LDL-cholesterol, fatty acids, insulin, glucose and iron accompanied with significant decrease in the levels of HDL-cholesterol, glycogen, haemoglobin and ferritin due to radiation exposure. Administration of garlic alone to the rats caused nonsignificant changes in the estimated parameters indicating its safe use, but the treatment with garlic to rats before radiation exposure ameliorated the changes induced by gamma irradiation and tended to normalize their levels.It could be concluded that garlic administration may has a beneficial role in restoring the biochemical disorders induced by 5 Gy gamma irradiation

  10. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

    Institute of Scientific and Technical Information of China (English)

    Shumaila Kiran; Arif Mahmood Bhutta; Bakhtyawar Ali Khan; Sobia Durrani; Muhammad Ali; Muhammad Ali; Furhan Iqbal

    2012-01-01

    Objective: To report and compare the hematological and serum biochemical profile of goat and sheep from Rahim Yar Khan district in Southern Punjab. Methods: One hundred and twenty blood samples (98 goat and 22 sheep) were collected and their blood glucose, hemoglobin and serum biochemical parameters, cholesterol, aspartate transaminase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were determined by using spectrophotometer. Parameters were compared between goat and sheep. Results: It was observed that glucose (P=0.001), LDH (P=0.001) and ALT (P=0.001) concentrations differed significantly between sheep and goats. The studied parameters were compared between buck and goats and also between young goats (less than 12 months old) and adults (more than 12 months old). It was found that age and gender did not affect these hematological and serum biochemical parameters.Conclusions: It can be concluded that sheep have overall higher values than goats for studied hematological and serum biochemical parameters.

  11. The Hypertrophic Marchigiana: physical and biochemical parameters for meat quality evaluation

    Directory of Open Access Journals (Sweden)

    F. M. Sarti

    2010-04-01

    Full Text Available The aim of this study was to evaluate the meat quality of double muscled Marchigiana young bulls characterized by different genotypes for the hypertrophy: normal and mutated (heterozygous. Calpain and calpastatin activities were determined to verify the state of aging meat on a sample of Longissimus thoracis muscle (XIII thoracic rib taken at slaughtering (0h and after 24 hours (24h. After 14 days of aging, another sample of muscle was taken to evaluate physical and chemical parameters of meat quality. The results showed a better meat quality of mutated animals respect normal animals. Another interesting result was the correlation between the biochemical parameters and some physical parameters, such as WBS (Warner Bratzler Shear Force, CL (Cooking loss. These results showed the relationship between the proteolytic activity of calpain system and meat tenderness.

  12. Seasonal variation of hemato-biochemical parameters in indigenous pig: Zovawk of Mizoram

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    P. Mayengbam

    2015-06-01

    Full Text Available Aim: To investigate the influence of season on erythrocyte picture, plasma enzyme and electrolyte profile of local pig of Mizoram at a different age. Materials and Methods: A volume of 2 ml of blood samples were collected from 72 Zovawk pigs of three different age groups viz. pre-weaning, grower and adult pigs reared in College of Veterinary Sciences and Animal Husbandry Selesih, Aizawl, Mizoram, India, livestock farm during summer and winter in order to find out influence of season on erythrocyte picture, enzyme and electrolyte profile. The hematological parameters were estimated by using an automatic blood analyzer. The biochemical parameters were estimated by using diagnostic kits. Results: The grower pigs had lower hemoglobin (Hb, packed cell volume (PCV, mean corpuscular volume (MCV, mean corpuscular Hb (MCH during summer, rainy and winter seasons (p<0.05 and lower MCH concentration (MCHC during summer season (p<0.05. Adult pigs had lower Hb with higher total erythrocyte count in rainy season. PCV and MCV of adult pigs decreased in summer (p<0.05. Serum glutamic oxaloacetic transaminase activity of both the grower and adult groups increased during summer and rainy seasons (p<0.05. Zovawk pigs had higher sodium (Na and lower potassium (K in rainy season (p<0.05. Pre-weaning piglets had higher Na, K, calcium (Ca, and magnesium (Mg in summer than in winter. Grower pigs had higher Na, K and Ca in summer (p<0.05. Pi of pre-weaning and grower groups was higher in winter. Adult pigs had lower alkaline phosphatase activity and Ca in summer and higher Pi and Mg in winter (p<0.05. Conclusion: Seasonal variation in some hemato-biochemical parameters of Zovawk pig were investigated. Influence of season on the hemato-biochemical profile was most evident during the grower stage, followed by adults and pre-weaning piglets.

  13. THE EFFECT OF LUTEIN ADDITIVES ON BIOCHEMICAL PARAMETERS IN BLOOD OF LAYING HENS

    Directory of Open Access Journals (Sweden)

    Manuela Grčević

    2016-06-01

    Full Text Available The aim of this study was to present the performance and some biochemical parameters in the blood of hens fed feed mixtures with different levels of lutein. The study involved a total of 291 Tetra SL hybrid laying hens, divided into 3 groups (O0 with 0 mg/kg lutein added, O200 with 200 mg/kg lutein added and O400 with 400 mg/kg lutein added, and lasted for 5 weeks. The O0 group included 93 hens, whereas experimental groups 98 and 100 hens, respectively. The highest egg production and the best feed consumption per egg were recorded in O400 group with a total of 3367 pieces of eggs produced and 128.60 g of feed consumed. The best laying intensity (96.68% and number of eggs per laying hen (34.19 as well as the highest feed consumption per day (129.01 g, was recorded in O200 group. Lutein added to feed mixtures for laying hens did not significantly affect the values of biochemical parameters in blood (P>0.05. An increase in total cholesterol (CHOL and HDL cholesterol content as well as the decrease of total protein (PROT and albumin (ALB content in the experimental groups can be observed. The content of glucose (GUK and urea was similar between the experimental groups, while the highest content of triglycerides (TGC was recorded in O200 group. It can be concluded that different levels of lutein added to the feed mixtures for laying hens did not have negative effect on the performance and blood biochemical parameters of laying hens.

  14. Prevalence and assessment of biochemical parameters of attention-deficit hyperactivity disorder children in Bangladesh

    Science.gov (United States)

    Hasan, Chowdhury Mohammad Monirul; Islam, Mohammad Monirul; Mahib, Muhammad Mamunur Rashid; Arju, Mahmud Ahmed Chowdhury

    2016-01-01

    Background and Objectives: This study aimed to evaluate some new biochemical parameters that help ensuring the early and precise diagnosis of attention-deficit hyperactivity disorder (ADHD) in blood plasma. Design and Settings: A prospective study conducted with patients scheduled for some new biochemical parameters that help ensuring the early and precise diagnosis of ADHD in blood plasma in a Child Development Center of the Chittagong, Bangladesh. Materials and Methods: The study was carried out at two levels. The first level was questionnaire on personal data and disease history while the second was on biochemical examination of the plasma ammonia and lactate status. A total of 100 children (age range 2 years 4 months to 12 years 6 months, mean age 7 years 5 months) were investigated in this study among 75 were male and 25 were female. This study was conducted in Chittagong Maa-O-Shishu General Hospital, Bangladesh. Results: We observed that the level of plasma ammonia and lactate were higher in ADHD children (36-60 μmol/L; P < 0.05 and 22-30 μmol/L; P < 0.05, respectively) compare to a reference value. The prevalence of ADHD is higher in male (75%) than in female (25%) with a ratio of 3:1. Consanguinity increases the risk of having ADHD in the next generation. Conclusion: This study concludes that there might be a correlation between ADHD and increased level of plasma ammonia and lactate level, and those might be an important parameter in the diagnosis of ADHD patients. PMID:27330258

  15. Haematological and Serum Biochemical Parameters of Local Turkey Poults Fed Diets Containing Two Varieties of Sorghum

    Directory of Open Access Journals (Sweden)

    E.B. Etuk

    2012-12-01

    Full Text Available These studies were conducted to determine the effects of two varieties of sorghum, Samsorg 17 and ICSV 400 on the haematological and serum biochemical parameters of local turkey breeds, reared in Nigeria. Two hundred and sixteen poults were divided into 9 treatment groups of 24 each, which were further replicated thrice and fed starter diets containing Samsorg 17 and ICSV 400. Similar (P > 0.05 RBC and PCV values were obtained with the two diets. Samsorg 17 fed poults produced lower, though not significantly (P > 0.05 serum albumin, glucose, urea, creatinine, sodium, chloride, ALP, SGPT and SGOT values than those on ICSV 400 diet. Higher RBC, MCHC, MCH, MCV and PCV values were observed with Samsorg 17 fed turkeys than those on ICSV 400 diets. Serum glucose and creatinine decreased and SGOT increased with dietary sorghum. Similar (p > 0.05 Hb, WBC, MCHC, MCV and PCV values were obtained in all groups. Values of serum biochemical indices assayed except urea, calcium, potassium and chloride showed no significant (p > 0.05 differences among the treatment groups. It was therefore concluded that Samsorg 17 and ICSV 400 sorghum varieties could sustain local turkey production without any on toward effects on their haematological and serum biochemical indices.

  16. Correlations between endogen amylin hormone and some hormonal, biochemical and bone parameters in pullets

    Directory of Open Access Journals (Sweden)

    S Guzel

    2014-12-01

    Full Text Available The objective of this study was to assess the correlations of amylin (a pancreatic polypeptide hormone with some hormonal, biochemical and bone parameters in pullets. Forty 18-week-old pullets were used. Plasma amylin, CT (calcitonin, 1,25 (OH2 vitamin D (1,25 dihydroxycholecalciferol , serum osteocalcin, glucose, ALP (alkaline phosphatase, cholesterol, and triglycerides, as well as weight, length and total volume of tibiotarsi were measured. Plasma amylin concentration was negatively correlated with serum cholesterol (p<0.05 and triglycerides (p<0.05 concentrations. Plasma amylin concentration was significantly and positively correlated with plasma calcitonin concentrations (p<0.001. Serum ALP and plasma amylin concentrations were positively correlated (p<0.01. There were no correlations between amylin hormone and other parameters. Based on these results, it is possible to conclude that endogen amylin may effect cholesterol, triglycerides, calcitonin, and ALP levels in pullets without changing some other hormonal, biochemical and bone parameters related to calcium and lipid metabolism.

  17. Evaluation of nutritional and biochemical parameters in spontaneously hypertensive rats following antihypertensive treatment

    Directory of Open Access Journals (Sweden)

    Joanna Suliburska

    2014-03-01

    Full Text Available Introduction. One side effect of antihypertensive drugs is their impact on nutritional status and metabolism. The purpose of this study was to assess the nutritional and biochemical parameters in spontaneously hypertensive rats following treatment with antihypertensive drugs. Material and methods. The experiment was performed on 50 male spontaneously hypertensive rats (SHR, which were assigned to fi ve groups: control (C, with perindopril (PR, with metoprolol (MT, with indapamide (ID, and with amlodipine (AM. All rats were provided ad libitum standard diet (with or without drugs and distilled water. After 45 days, the animals were weighed and killed. Liver, kidney, heart, spleen, pancreas, and blood samples were collected. Concentrations of glucose, cholesterol, triglycerides, and albumin were assayed in serum. Morphology parameters, such as white blood cell, red blood cell, hematocrit, and lymphocyte counts were measured in the blood. Blood pressure was measured using a tail-cuff plethysmograph. Results. The results obtained indicate that the hypotensive drugs under investigation had no effect on the selected nutritional parameters. Perindopril signifi cantly decreased the relative mass of the heart and amlodipine markedly decreased the relative mass of the pancreas. A markedly higher concentration of glucose in the group with indapamid, and a signifi cantly lower concentration of triglycerides in the group with metoprolol, were observed. Indapamide and amlodipine markedly increased the value of red blood cells and hematocrit in the blood of SHR. Conclusions. Long-term therapy with antihypertension drugs may infl uence tissue mass and biochemical and morphological status in the body.

  18. Investigation of hematological and biochemical parameters in obese women in reproductive age

    OpenAIRE

    Kara IH et al.

    2012-01-01

    Objective: The purpose of this article is to analyze the relationship between the body mass indices and hematological and biochemical parameters of reproductive women at the age of 18 to 49 years old.Method: This cross-sectional and descriptive study includes 124 consecutive reproductive women patients at the age between 18 and 49 and the mean age is 33.6±7.3, admitted to Düzce University Faculty of Medicine, Department of Family Medicine Obesity and Check-up Outpatient Clinics between June 2...

  19. Changes in biochemical, strength, flexibility, and aerobic capacity parameters after a 1700km ultraendurance cycling race

    OpenAIRE

    Vicente Javier Clemente-Suarez

    2014-01-01

    The purpose of the present research was to study the organic response after ultraendurance cycling race. Selected biochemical, leg strength, flexibility, and aerobic capacity parameters were analyzed in 6 subjects 5 days before and 5 days after completing a 1700 km ultraendurance cycling race. After the race, participants presented a significant decrease in Hb (167.8 ± 9.5 versus 141.6 ± 15.7 mg/dL), strength (29.4 ± 2.7 versus 25.5 ± 3.7 cm in a countermovement jump), and oxygen upt...

  20. Effect of fire smoke on some biochemical parameters in firefighters of Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Rezq Ameen M

    2008-12-01

    Full Text Available Abstract Background Firefighters who are facing fires, are frequently exposed to hazardous materials including carbon monoxide, hydrogen cyanide, hydrogen chloride, benzene, sulphur dioxide, etc. This study aimed to evaluate some relevant serum biochemical and blood hematological changes in activity involved firefighters in comparison to normal subjects. Subjects and Methods Two groups of male firefighters volunteered to participate in the study. The first included 28 firefighters from Jeddah, while the second included 21 firefighters from Yanbu, with overall age ranged 20–48 years. An additional group of 23 male non-firefighters volunteered from both cities as normal control subjects, of age range 20–43 years. Blood samples were collected from all volunteer subjects and investigated for some relevant serum biochemical and blood hematological changes. Results The results obtained showed that, there were statistically significant differences in liver function, kidney function, serum lipid profile, cortisol, creatine kinase, lactate dehydrogenase, iron and its biologically active derivatives, and blood picture in firefighters as compared with the normal control group. These results indicate that, fire smoke mainly affects serum biochemical and blood hematological parameters. Such results might point out to the need for more health protective and prophylactic measures to avoid such hazardous health effects that might endanger firefighters under their highly drastic working conditions. Conclusion Besides using of personal protective equipments for firefighters to protect them against exposure to toxic materials of fire smoke, it is recommended that, firefighters must be under continuous medical follow up through a standard timetabled medical laboratory investigations to allow for early detection of any serum biochemical or blood hematological changes that might happen during their active service life and to allow for early treatment whenever

  1. Linking transient storage parameters to exchange mechanisms and reach characteristics

    Science.gov (United States)

    Morén, Ida; Wörman, Anders; Riml, Joakim

    2015-04-01

    transient storage model. Important geomorphologic and hydraulic features used as classification parameters are those that that can be directly linked to specific management measures implemented in streams to increase natural remediation of nutrients and other contaminants. Only field data from reaches where measurements independent of the tracer tests have been done is included in this study in order to correctly analyse dominating mechanisms and combination of mechanisms and to be able to link the retention times to relevant and measurable reach characteristics.

  2. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    OpenAIRE

    R.N. Sani; M. Moezifar

    2016-01-01

    This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring), August and October (401 samples in summer). California Mastitis Test (CMT), milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial cultur...

  3. Modeling photosynthesis of discontinuous plant canopies by linking Geometric Optical Radiative Transfer model with biochemical processes

    Science.gov (United States)

    Xin, Q.; Gong, P.; Li, W.

    2015-02-01

    Modeling vegetation photosynthesis is essential for understanding carbon exchanges between terrestrial ecosystems and the atmosphere. The radiative transfer process within plant canopies is one of the key drivers that regulate canopy photosynthesis. Most vegetation cover consists of discrete plant crowns, of which the physical observation departs from the underlying assumption of a homogenous and uniform medium in classic radiative transfer theory. Here we advance the Geometric Optical Radiative Transfer (GORT) model to simulate photosynthesis activities for discontinuous plant canopies. We separate radiation absorption into two components that are absorbed by sunlit and shaded leaves, and derive analytical solutions by integrating over the canopy layer. To model leaf-level and canopy-level photosynthesis, leaf light absorption is then linked to the biochemical process of gas diffusion through leaf stomata. The canopy gap probability derived from GORT differs from classic radiative transfer theory, especially when the leaf area index is high, due to leaf clumping effects. Tree characteristics such as tree density, crown shape, and canopy length affect leaf clumping and regulate radiation interception. Modeled gross primary production (GPP) for two deciduous forest stands could explain more than 80% of the variance of flux tower measurements at both near hourly and daily time scales. We also demonstrate that the ambient CO2 concentration influences daytime vegetation photosynthesis, which needs to be considered in state-of-the-art biogeochemical models. The proposed model is complementary to classic radiative transfer theory and shows promise in modeling the radiative transfer process and photosynthetic activities over discontinuous forest canopies.

  4. Influence of Fasciola Hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

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    A Hodžić

    2013-03-01

    Full Text Available Background: The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver.Methods: Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n=34 and infected group (n=29. For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used.Results: Biochemical analysis of tested parameters showed a significant elevation (P≤0.05 of serum gamma-glutamyl transferase (GGT, total bilirubin (TBIL and direct bilirubin (DBIL in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST between groups. Vascular and biliary systems of the liver were found to be affected.Conclusion: Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica.

  5. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

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    Prokić Marko D.

    2015-01-01

    Full Text Available Aspartame (ASP is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH, as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO and lipid peroxides (LPO in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  6. Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

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    Frelich Angela

    2008-09-01

    Full Text Available Abstract D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.

  7. Oxamyl-induced alterations in hematological and biochemical parameters in rats

    International Nuclear Information System (INIS)

    Effect of daily oral doses of 0.9 and 2.5 mg/kg of the carbamate insecticide oxamyl for 16 days on selected hematological and biochemical parameters in male rats was investigated. The weight of animals was significantly decreased compared to controls. The hematological studies revealed significant reduction in red blood cell count, hemoglobin and hematocrit values. Impairment of thyroid function was noticed by elevation of triiodothyronine (T3) and depression of thyroxine (T4). Brain acetylcholinesterase (AchE) was moderately inhibited in the first few days of exposure. However, the results of the parameters investigated indicate a moderate degree of toxicity of oxamyl following oral exposure of the doses selected

  8. Changes in biochemical, hemodynamic, and dialysis adherence parameters in hemodialysis patients during Ramadan.

    Science.gov (United States)

    Alshamsi, Shaikha; Binsaleh, Fatima; Hejaili, Fayez; Karkar, Ayman; Moussa, Dujana; Raza, Hamad; Parbat, Parkash; Al Suwida, Abdulkareem; Alobaili, Saad; AlSehli, R; Al Sayyari, Abdulla

    2016-04-01

    This paper aimed to study the effect of Ramadan fasting on biochemical and clinical parameters and compliance for dialysis. A prospective multicenter observational cross-sectional study comparing fasting with a non-fasting stable adult hemodialysis patients for demographic and biochemical parameters, compliance with dialysis, inter-dialytic weight gain, pre- and post-blood pressure, and frequency of intradialytic hypotensive episodes was carried out. Six hundred thirty-five patients, of whom 64.1% fasted, were studied. The fasters were younger (53.3 ± 16.2 vs. 58.4 ± 16.1 years; P = 0.001) but had similar duration on dialysis (P = 0.35). More fasters worked (22.0% vs. 14.6%; P = 0.001) and missed dialysis sessions during Ramadan. No differences were noted between groups in sex, diabetic status, or dialysis shift or day. There were no differences in the pre- and post-dialysis blood pressure; serum potassium, albumin or weight gain; diabetic status; sex; and dialysis shift time or days. However, serum phosphorous was significantly higher in the fasting group (2.78 ± 1.8 vs. 2.45 ± 1.6 mmol/L; P = 0.045). There were no intragroup differences in any of the parameters studied when comparing the findings during Ramadan with those in the month before Ramadan. Fasters were significantly younger and more likely to be working, to miss dialysis sessions, and to have higher serum phosphorous levels. No other differences were observed. PMID:26420510

  9. Hybrid Differential Evolution for Estimation of Kinetic Parameters for Biochemical Systems

    Institute of Scientific and Technical Information of China (English)

    ZHAO Chao; XU Qiaoling; LIN Siming; LI Xuelai

    2013-01-01

    Determination of the optimal model parameters for biochemical systems is a time consuming iterative process.In this study,a novel hybrid differential evolution(DE)algorithm based on the differential evolution technique and a local search strategy is developed for solving kinetic parameter estimation problems.By combining the merits of DE with Gauss-Newton method,the proposed hybrid approach employs a DE algorithm for identifying promising regions of the solution space followed by use of Gauss-Newton method to determine the optimum in the identified regions.Some well-known benchmark estimation problems are utilized to test the efficiency and the robustness of the proposed algorithm compared to other methods in literature.The comparison indicates that the present hybrid algorithm outperforms other estimation techniques in terms of the global searching ability and the convergence speed.Additionally,the estimation of kinetic model parameters for a feed batch fermentor is carried out to test the applicability of the proposed algorithm.The result suggests that the method can be used to estimate suitable values of model parameters for a complex mathematical model.

  10. Changes in biochemical parameters on the first day after kidney transplantation: risk factors for nosocomial infection?

    Institute of Scientific and Technical Information of China (English)

    YANG Yi; REN Liang; ZHANG Yong; LIU Hang; CAO Bin; ZHANG Xiao-dong

    2010-01-01

    Background Nosocomial infection in early post-transplantation period is a tough problem for kidney transplantation. Few reports have explored the relations between biochemical parameters and nosocomial infection in kidney transplantation. This retrospective study was carried out to describe the characteristics of nosocomial infection in the very early period of kidney transplantation and to determine the risk factors in biochemical parameters and their alterations. Methods Patients who underwent their first kidney transplantation from January 2001 to March 2009 in Beijing Chao-Yang Hospital were recruited and the nosocomial infectious episodes were collected for this study. Gender, age, donor type, delayed graft function (DGF) and biochemical parameters such as serum uric acid, lipids files and albumin on day 0 (before transplantation) and day 1 (24 hours after transplantation) and their changes were analyzed with Logistic regression models for nosocomial infection. Results A total of 405 patients (315 men and 90 women) were involved in this study. There were 80 patients experiencing 113 infection episodes and 105 strains of microorganism were indentified. In univariate analysis, there were significant differences in DGF, albumin on day 0, lipoprotein (a) (Lp(a)) on day 1, change in low density lipoprotein-cholesterol (LDL-C, day 1-day 0) and change in uric acid (day 1-day 0) between nosocomial infection patients and noninfectious patients (P<0.05). In multivariate analysis, change in uric acid (day 1-day 0) (Off 5.139, 95% Cl 1.176-22.465, P<0.05), change in LDL-C (day 1-day 0) {OR4.179, 95% Cl 1.375-12.703, P<0.05) and DGF (Of? 14.409, 95% Cl 1.603-129.522, P<0.05) were identified as independent risk factors for nosocomial infection in kidney transplantation. Conclusions Most nosocomial infections in early postoperative period of kidney transplantation are bacterial, especially with Gram-negative bacteria. The most common infection sites are respiratory tract

  11. Effect of Root Kudzu on Biochemical Parameters in Streptozotocin-Induced Diabetic Rats

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    Abas Rashidi

    2015-04-01

    Full Text Available Introduction: Diabetes, characterized by high blood glucose levels, is a serious chronic disease and antioxidant play an important role in the pathophysiology, complications, and management of the disease. Kudzu root, a traditional Chinese herbal medicine, has a long history of use for the treatment of headaches, cough, edema and extracts are a rich source of isoflavones and antioxidants property. The aim of this study was to evaluate the effect of the kudzu root on biochemical parameters in streptozotocin-induced diabetic rats. Methods: Male wistar rats were divided into four groups (n=32 including: Control, diabetic, diabetic rats treated with 100 mg/kg kudzu root extract, and diabetic rats treated with 50mg/kg kudzu root extract. Diabetes was induced by STZ intraperitoneally administration (50 mg/kg and kudzu root extract was gavaged at the dose of 50 and 100 mg/kg for five weeks. Blood biochemical factors and insulin serum levels were also assessed. Results: The results show that in STZ induced diabetic rats, the serum levels of FBS, total cholesterol (TC and triglyceride (TG increased whiles the levels of HDL and Insulin were decreased. Moreover in diabetic kudzu treatment groups, the serum levels of of FBS, total cholesterol (TC and triglyceride(TG decreased (P≤0.001 whiles the serum level of HDL and Insulin were increased. Conclusion: In addition the antioxidant effects, Kudzu root extract produced a significant anti-hyperglycemic effect on STZ-induced diabetic rats.

  12. Haematological and biochemical blood parameters in horses used for sport and recreation

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    Katarzyna BURLIKOWSKA

    2015-12-01

    Full Text Available The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P<0.05 red blood cell count, haematocrit value and haemoglobin concentration as well as the serum level of total protein, albumin, α2- and γ-globulin, HDL and iron. The level of β- globulin, magnesium and activity of creatine kinase were significantly higher in recreationally used horses. There were no differences in the values of red blood cell parameters (MCV, MCH, MCHC, number of leucocytes and the percentage of their respective kinds, platelets, concentration of urea, creatinine, glucose, triglycerides, total cholesterol, LDL, activity of AST, ALT, LDH and in the content of Na, K, Cl, P, Ca between the tested groups. The results of the present study indicate that the type of training influences some of the resting haematological and biochemical indices in adult horses. Horses used for show jumping have higher aerobic capacity and show a more beneficial lipid profile compared to horses used for recreation.

  13. The Effects of Ramadan Fasting and Physical Activity on Blood Hematological-Biochemical Parameters

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    Keyvan Hejazi

    2012-11-01

    Full Text Available Introduction: Fasting during Ramadan is a religious obligation and belief for healthy and adult Moslems. The aim of the study was to determine the effects of Ramadan fasting and physical activity on blood hematological-biochemical parameters. Methods: In this study twenty six healthy males were assigned into two experimental groups, non-active fasting (13 people and active fasting (13 people groups. Two blood samples were taken from each group at the beginning and end of Ramadan. The data was analyzed through repeated measure ANOVA.Results: At the end of Ramadan, Hematocrit (Hct, red blood cell count (RBC, TC, LDL, HDL, LDL: HDL, TC: HDL and VLDL decreased significantly in both groups (P<0.05. Amount of HDL during fasting increased significantly in active fasting (P=0.023 and non-active fasting (P=0.042. The FBS levels reduced significantly only in active fasting group (P<0.05. Conclusion: Fasting during Ramadan by regular physical activity led to the positive alterations in Hematological-Biochemical Index. These changes can be due to the alterations in diet, biology response of the body to the starving and physical activity during Ramadan.

  14. Variation in biochemical parameters in different parts of potato tubers for processing purposes.

    Science.gov (United States)

    Bandana; Sharma, Vineet; Kaushik, S K; Singh, Brajesh; Raigond, Pinky

    2016-04-01

    The present study was conducted to estimate the variation in bio-chemical parameters among eight different parts viz. bud end cortex, bud end medulla, central cortex, central medulla, pith, stem end cortex, stem end medulla and peel of potato tuber of processing varieties. Concentration of dry matter, reducing sugar, sucrose and starch content were higher in cortical region than in medullar region of stem end, bud end and central portion. Variety Kufri Chipsona-1 had maximum dry matter content in stem end cortex (SEC 30.34 %), followed by Kufri Frysona (SEC 27.71 %). Mean reducing sugar values were comparatively more in bud end cortex (BEC 111.3 mg/100 g Fresh Weight) and lowest in stem end medulla (SEM 44.05 mg/100 g FW). Bio-chemical contents varied considerably within different parts of tuber as well as in different genotypes. The information generated in this study can help processors for effective utilization of potato for various types of processing products viz., chips and French fries. PMID:27413232

  15. Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis

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    Bijan Esmaeilnejad

    2012-03-01

    Full Text Available Babesia ovis plays an important role in causing anemia and kidney dysfunction in affected animals. There are few extensive studies about hematological and biochemical findings of small ruminants' babesiosis caused by B. ovis. The aim of this study was to evaluate the effect of babesiosis on some hematological and biochemical parameters in infected small ruminants with B. ovis. A total of 280 sheep and 122 goats from 40 herds were randomly examined for the presence of B. ovis in blood samples. Of 402 samples, 67 animals (16.7% were positive for B. ovis of which 52 (18.5% were sheep and 15 (12.2% goats, respectively. The infected animals were divided into four subgroups according to parasitemia rates (<1%, 1%, 2%, and 3%. As a control group, 67 uninfected animals were also selected from the same farms. With increase in parasitemia rates, hemoglobin concentration (Hb, packed cell volume (PCV, red blood cells (RBCs, mean corpuscular volume (MCV and mean corpuscular hemoglobin concentration (MCHC significantly decreased (P < 0.05, while, total leukocyte count, number of lymphocyte, monocyte, neutrophil and eosinophil showed a significant increase (P < 0.05. Infected animals presented a significant elevation (P < 0.05 of total proteins and significantly lower level (P < 0.05 of albumin compared to non-infected animals. Significant elevation (P < 0.05 of BUN, creatinine, cholestrol, triglyceride, HDL and LDL level were found with parasitemia progression.

  16. Hematologic and serum biochemical parameters of apparently healthy rescued formosan pangolins (Manis pentadactyla pentadactyla).

    Science.gov (United States)

    Chin, Shih-chien; Lien, Chen-yah; Chan, Yating; Chen, Chun-lin; Yang, Yi- ching; Yeh, Lih-seng

    2015-03-01

    Natural habitats of pangolins are rapidly deteriorating because of extensive farming, logging, and human construction activities. In addition, the illegal trading of pangolins substantially accelerated the decline of the pangolins' population in southeastern Asia. The maintenance of confiscated pangolins in rescue centers is currently a daunting task for veterinarians and conservation biologists. There is limited information in the literature about the reference values regarding the physiology of pangolins. The purpose of this study is to establish reliable hematologic and serum biochemical reference values for the Formosan pangolin (Manis pentadactyla pentadactyla). Blood samples were collected from 51 apparently healthy pangolins from a population of 117 rescued pangolins at the Taipei Zoo. Sex-related differences were observed in platelet count, alanine aminotransferase level, mean corpuscular hemoglobin concentration, and total protein level. Age-related differences were also noted; juveniles have significantly higher platelet counts and alkaline phosphatase levels than their adult counter parts. The hematologic and serum biochemical reference values for the Formosan pangolin presented in this study can be applied in the medical care of this important species during rescue attempts. It is the first systematic report of blood parameters of apparently healthy pangolins and provides a basis for future investigation of this species. The reference values reported in this study may also be applicable to other pangolin species in the genus Manis. PMID:25831578

  17. NOVEL INVESTIGATIONS ON THUJA OCCIDENTALIS EXTRACT ON RABBIT HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

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    Mansoor Ahmad

    2013-03-01

    Full Text Available Thuja occidentalis (family: Cupressaceae has been used as antiseptic, expectorant, diuretic and in the treatment of cancer and removal of kidney stones in different systems of medicine. The present studies were carried out on T. occidentalis leaves extract to evaluate the hematological and biochemical effects on rabbits. The animals were divided in to control (A and B and treated (C and D male and female groups (n=5. There was a slight decrease in Hemoglobin, Red blood cell count and Hematocrit/Packed cell volume values, while a significant increase in platelet count was observed in male and female treated groups. However, slight increase in total leukocyte count value in male treated group and decrease count with female treated group was also observed. Urea, Creatine phospho kinase, Creatine phospho kinase iso-enzyme, Direct Bilirubin, Gamma-glutamyl transpeptidase, Cholesterol and Albumin/Globulin ratio values were elevated in male treated group. While Phosphorus, Uric acid, Lactate dehydrogenase, Serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, Alkaline Phosphatase, Cholesterol, Triglycerides, High density lipoprotein, Low density lipoprotein, Very-low-density lipoprotein, Globulin and Blood Glucose values were found low. In female treated group almost same results were observed like male treated group except that hypoglycemic effect was more pronounced in female treated group. These findings suggested that when results of hematological and biochemical parameters of test groups (C & D were compared with their respective controls (A & B, significant changes were observed which contributes towards the diversified therapeutic effect of T. occidentalis.

  18. Effect of salinity on growth, biochemical parameters and fatty acid composition in safflower (carthamus tinctorius l.)

    International Nuclear Information System (INIS)

    The aim of the present project is to investigate the effect of salinity on growth, biochemical parameters and fatty acid composition in six varieties of safflower as well as identification of stress tolerant variety under saline (8 d Sm-1) condition. It was observed that salinity significantly decreased the dry weight and fresh weight of safflower varieties. Nitrate reductase (NRA) and nitrite reductase (NiRA) activities were also reduced in response to salinity in all safflower genotypes but Thori-78 and PI-387820 showed less reduction which could be a useful marker for selecting salt tolerant varieties. Under salinity stress, total free amino acids, reducing, non reducing sugars and total sugars increased in all varieties. Accumulation of sugars and total free amino acids might reflect a salt protective mechanism and could be a useful criterion for selecting salt tolerant variety. Comparison among safflower genotypes indicated that Thori-78 and PI-387820 performed better than the others and successful in maintaining higher NRA, NiRA and other metabolites thus were tolerant to salinity. Differential effect upon fatty acid synthesis was observed by different varieties under salinity stress but PI-170274 and PI-387821 varieties better maintained their fatty acid composition. It can be concluded from present studies that biochemical markers can be used to select salinity tolerant safflower varieties. (author)

  19. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss Fry

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    Salman Rauoof CHALKOO

    2013-01-01

    Full Text Available This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocytes (WBC and erythrocytes counts (RBC, hemoglobin (Hb content, hematocrits (PCV, leukocytes differential count and blood indices such as MCV, MCH and MCHC. Also blood serums were analysed for total protein (TP and blood enzymes. All the calculations were made using the SPSS© and t-test statistical method. In hematological findings nine parameters were revealed significant differences (P

  20. Effect of induced epilepsy on some biochemical parameters in female rats

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    J.S. H. Ali

    2012-01-01

    Full Text Available The activity of cholinesterase and some biochemical parameters of blood such as glucose, cholesterol and phospholipids were estimated in 52 epilepsy induced females of Wister albino rats. Animals of this experiment were divided into two groups, group (I regarded as control and group (II administrated subcutaneously by pentylenetetrazole 100mg/kg and divided in to three sub-groups according to the time of samples collection 3 hrs, 24 hrs and 1 week. The results revealed that epilepsy induction caused a significant inhibition of serum cholinesterase activity 3 hrs after induction while in the brain, the activity of cholinesterase was significantly increased after 24 hrs Serum glucose level was significantly elevated after 3 hrs and 24 hrs of induction, total cholesterol and phospholipids were not changed. From the results obtained in this study, it can be concluded that epilepsy caused significant changes in cholinesterase activity in brain and serum in addition to the glucose level in the serum.

  1. Effects of sodium ferrous chlorophyll treatment on anemia of hemodialysis patients and relevant biochemical parameters.

    Science.gov (United States)

    Xu, X F; Hu, J P; Cheng, X; Yu, G J; Luo, F; Zhang, G S; Yang, N; Shen, P

    2016-01-01

    This study explores the effects of sodium ferrous chlorophyll treatment on the anemia of maintenance hemodialysis (MHD) patients, as well as the relevant biochemical parameters. We selected 72 patients who had received regular MHD treatment two or three times a week for more than 3 months in the Hospital of Traditional Chinese Medicine of Zhengzhou City of Henan Province from March 2014 to March 2016. They were equally divided into a treatment group and a control group. Haemoglobin (HB) and hematocrit (HCT) of the treatment group increased significantly after treatment (p 0.05). Albumin (ALB) dosage of the treatment group increased after treatment (p 0.05). ALB level of the treatment group increased more significantly than in the control group (p MHD patients and their quality of life. PMID:27049084

  2. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA) COLLECTED IN THE INACTIVE SEASON

    OpenAIRE

    AGRIPINA SAPCALIU; I. RADOI; D. CONDUR; A. SICEANU; ELIZA CAUIA; CRENGUTA PAVEL

    2013-01-01

    The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera) in inactive...

  3. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

    OpenAIRE

    AGRIPINA SAPCALIU; ELIZA CAUIA; ION GURESOAIE; DAN CONDUR; ION RADOI

    2013-01-01

    Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination). The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth...

  4. Evaluation of bone metabolism in newborn twins using quantitative ultrasound and biochemical parameters.

    Science.gov (United States)

    Kara, Semra; Güzoğlu, Nilüfer; Göçer, Emine; Arıkan, Fatma Inci; Dilmen, Uğur; Dallar Bilge, Yıldız

    2016-03-01

    Metabolic bone disease (MBD) is one of the important complications of prematurity. Early and adequate nutritional interventions may reduce the incidence and potential complications of MBD. The present study aimed to evaluate bone metabolism in twins via biochemical parameters and quantitative ultrasound (QUS) and to compare the results between twin pairs. Moreover, twin infants were evaluated in terms of potential risk factors likely to have impact on MBD. Forty-three pairs of twins were included in the study. Serum calcium, phosphorus, magnesium, and alkaline phosphatase concentrations were assessed and bone mineral density was measured using QUS (speed of sound, SOS) at postnatal 30 d. Co-twin with the higher birth weight was assigned to Group 1 (n = 36) and the other twin was assigned to Group 2 (n = 36). Birth weight and head circumference were significantly higher in the infants of Group 1 compared with Group 2. No significant difference was found among the groups in terms of gender, history of resuscitation, length of stay in intensive care unit (ICU) or in the incubator, duration of total parenteral nutrition (TPN), type of nutrition, vitamin D use, biochemical parameters, and the SOS value. The factors likely to affect SOS, including type of pregnancy, maternal drug use, gender of infant, birth weight, head circumference at birth, gestational week, length of stay at the ICU, duration of TPN, type of nutrition, resuscitation, vitamin D use, and levels of calcium, phosphorus, magnesium, and alkaline phosphatase were entered into the model. The phosphorus level and the maternal drug use were found to be the factors that significantly reduced SOS, whereas pregnancy after assisted reproductive techniques was found to be a significant enhancing factor. PMID:25777793

  5. THE EFFECTS OF VEGETABLE LECITHIN ON SOME SANGUINE BIOCHEMICAL PARAMETERS AT LACTATING SHEEP AND GOATS

    Directory of Open Access Journals (Sweden)

    D. DRINCEANU

    2013-12-01

    Full Text Available From a complex experiment of establishing the effects of lecithin supplemented in the diet of small ruminants, in this paper we present the influence of lecithin on some sanguine biochemical parameters at lactating sheep and goat. The researches were made on a experimental group formed by 5 sheep from łurcana breed and 5 goats from Crapatina breed, which were fed in the first experimental period with a base ratio (Br formed by 1.5 kg of hill hay and 0.2 kg concentrate forage mixture, in the second experimental period the Br was supplemented with 100 g lecithin/day/animal. At the end of each experimental period were taken blood samples to establish some biochemical parameters from sanguine serum. The supplementation of base ration with 100 g lecithin/day/animal at lactating sheep and goats did not produced significant changes to values of total proteins (TS, albumin, creatinine, urea and total bilirubins (TB from sanguine serum. Contrary to expectation the cholesterol and triglycerides were higher than normal both in sheep (from 82 mg/dl to 83 mg/dl for cholesterol and from 23 mg/dl to 45 mg/dl for triglycerides but specially in goats (from 59 mg/dl to 68 mg/dl for cholesterol and from 24 mg/dl to 55 mg/dl for triglycerides. Were registered significant increment of activity of alkaline phosphatase and in γ – GT and a decrease of plasmatic activity of pancreatic α-amylase both in sheep and goats.

  6. Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).

    Science.gov (United States)

    Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

    2014-11-01

    The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality. PMID:25522520

  7. Investigation of some hematological and blood biochemical parameters in cattle spontaneously infected with bovine leukosis virus

    Directory of Open Access Journals (Sweden)

    Sandev Nikolay

    2013-09-01

    Full Text Available The aim of the present study was to follow out the alterations in some haematological and blood biochemical parameters in cattle spontaneously infected with enzootic bovine leukosis virus with regard to the invivodifferentiation of bovine leukosis stages. The experiment included 76 cows at various ages and body weight. Serological leukosis tests were done by agar-gel immunodiffusion test with a commercial kit of Synbiotiсs (France, containing standardised gp 51 antigen and positive serum approved by the EU. On the basis of haematological results, the cows were divided into three groups: first group – EBL-seropositive with normal haemogramme; second group – EBL seropositive with altered haemogramme and third group – controls. In cows from the first and the second group, a statistically significantly increased blood cell counts was established compared to healthy controls. The total WBC were increased in the second group (leukocytosis up to 33.21×109/l vs reference range of 5-10×109/l as well as lymphocyte percentages (lymphocytosis – 81.89% (reference 40–63%. A reduction in the proportion of neutrophils to 12.78% (relative neutropenia vs the reference range of 22-49% and monocytes (monocytopenia to 1.78% (reference range 2–6% was observed. A statistically significant reduction in Ca concentrations (4.41 mg/dl and higher inorganic phosphate levels (5.28 mg/dl were established in cows from the second group. Also, ASAT activity was considerably lower – 47.03 U/l, while alkaline phosphatase increased slightly within the reference range up to 167.68 U/l and 165.81 U/l in groups one and two, respectively. The present haematological and whole blood/serum biochemical results in cows spontaneously infected with EBL virus could be used as prognostic markers of the course of the disease, to distinguish the stages of infection with regard to alive diagnostics.

  8. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

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    Fabrícia Souza Predes

    2009-04-01

    Full Text Available This study was carried out to evaluate the effects of Arctium lappa L. (burdock on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST, alanine transaminase (ALT, gamma glutamyl transferase (GGT, total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.A bardana (Arctium lappa L é uma planta trazida do Japão e aclimatada no Brasil, e é extensamente utilizada na medicina popular em todo mundo. Este estudo foi realizado para avaliar os possíveis efeitos da A. lappa no fígado e nos parâmetros bioquímicos plasmáticos em ratos Wistar adultos. Estes receberam a infusão de bardana nas doses de 10 ou 20 g de folhas secas /L de água, por 40 dias. Não houve alteração significativa nos níveis plasmáticos de albumina, aspartato transaminase (AST, alanina transaminase (ALT, gamma glutamil transferase (GGT, proteínas totais, colesterol total, uréia, ácido úrico, triglicérides, cálcio, fósforo, bilirrubina direta e cloro. A análise morfológica não revelou alterações histopatológicas no fígado. Os dados bioquímicos e morfológicos não indicaram a toxicidade da bardana.

  9. Effect of ferrous sulphate on haematological, biochemical and immunological parameters in neonatal calves

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    Lobna S. Elgebaly

    2010-09-01

    Full Text Available The effect of oral administration of iron on haematological, biochemical and immunological parameters in neonatal calves was studied. Ten calves from a private farm in Gharbia Governorate were used. Calves were separated from their dams immediately after birth and received colostrum during the first hours after calving and twice daily for 48 h. Thereafter, they received whole milk. Calves were divided into two equal groups. The first group was kept as controls. Calves of the second group were given ferrous sulphate at a dose of 250 mg/calf daily, beginning at one day of age; this was continued for 28 days. Three blood samples were collected from each calf in all groups at 14, 21, 28 and 35 days of age. Iron administration produced a significant increase in red blood cell count, haemoglobin, packed cell volume and blood indices, in addition to non-significant changes in total and differential leukocyte counts. The administration of iron resulted in a significant increase in serum iron, total proteins, globulins, thyroid hormones, lymphocyte stimulation index, phagocytosis, body weight and body gain. The administration of iron is suggested as routine practice in calf-producing farms due to its advantageous effects on the parameters tested.

  10. Women with metabolic syndrome improve antrophometric and biochemical parameters with green banana flour consumption

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    Sandra Tavares da Silva

    2014-05-01

    Full Text Available Background: Consumption of green banana flour (GBF may promote health benefits, such as, decreased appetite, weight loss, glycemic control, intestinal function and lipid profile improvement, aging delay, cancer and heart disease prevention. The aim of this study was to evaluate the effects of green banana flour consumption on anthropometric and biochemical parameters in overweight women. Methods: The glycemic index of flour in the study was determined. The effects of consumption of 20 g of green banana flour/day on weight, body mass index (BMI, blood pressure, waist and hip circumference, body composition, hemoglobin, lipid profile, glucose, insulin, insulin resistance, liver function and energy intake were evaluated in 25 overweight women for 45 days. Results: The glycemic index of the flour under study was classified as low. Reduction (p < 0.05 in systolic blood pressure, hip circumference and fasting glucose levels were found in women who had metabolic syndrome criteria. Conclusions: Consumption of green banana flour (20 g/day for 45 days did not promote weight loss or changes in body composition in overweight women. It was noted, however, decreased hip circumference. Significant health parameter improvements were also noted in individuals with metabolic syndrome, which showed a reduction in systolic blood pressure and fasting glucose.

  11. Influence Of Feminine Hormones On Some Biochemical Parameters In Early And Late Menopausal Status

    International Nuclear Information System (INIS)

    Forty two healthy Egyptian women were participated in this study to evaluate the effect of early and late menopause on some biochemical and hormonal parameters. The women were divided into three equal groups. The first was the pre-menopausal group with regular menstrual cycle, the second was the late menopausal group comprised the menopausal female less than 5 years and the third group consisted of women that became menopause since 5-9 years. The second and third groups had almost the same age and body weight. After clinical examination, fasting blood samples were collected from all volunteers. Regarding the pre-menopausal women, blood was withdrawn between the 3rd and the 5th day post-menstruation (follicular phase). Follicle stimulating hormone (FSH), leutinizing hormone (LH), progesterone, prolactin (PRL), testosterone, leptin and thyroxine (T4) in addition to hemoglobin (Hb), blood glucose, calcium (Ca), inorganic phosphorous (P), magnesium (Mg) and uric acid were determined. Both menopausal groups showed significant increase in FSH and LH and significant decrease in PRL and late menopausal group experienced also multiple significant correlations between FSH, LH and other tested parameters. Prolongation of menopause exhibited decrease in leptin being significant as compared to pre-menopausal women than that experienced menopause at younger age. Due to the relation between leptin hormone and osteoporosis, the results of this investigation denoted that early cessation of annulations (menopause) in Egyptian women may lead to disturbance in bone metabolism causing inhibiting osteoblastogenesis and decreasing bone mass.

  12. Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

    Energy Technology Data Exchange (ETDEWEB)

    Smutna, M.; Hilscherova, K.; Paskova, V. [Masaryk Univ., Brno (CZ). RECETOX (Research Centre for Environmental Chemistry and Ecotoxicology); Marsalek, B [Czech Academy of Science, Brno (Czech Republic). Centre for Cyanobacteria and their Toxins

    2008-06-15

    Background, aim, and scope Restoration of lakes and reservoirs with extensive cyanobacterial water bloom often requires evaluation of the sediment quality. Next to the chemical analysis of known pollutants, sediment bioassays should be employed to assess toxicity of the present contaminants and to make predictions of associated risk. Brno reservoir in the Czech Republic is a typical example of water bodies with long-term problems concerning cyanobacterial water blooms. Comprehensive assessment of reservoir sediment quality was conducted since successful reservoir restoration might require sediment removal. An important part of this survey focused on an examination of the utility of Tubifex tubifex and its sublethal biochemical markers for the assessment of direct sediment toxicity. Materials and methods This complex study included chemical analysis of contaminants (heavy metals, organic pollutants), ecotoxicity testing of sediment elutriates (tests with Daphnia magna, Pseudomonas putida, Sinapis alba, Scenedesmus subspicatus), and other parameters. We have tested in more detail the applicability of T. tubifex as a test organism for direct evaluation of contact sediment toxicity. Survival tests after 14 days of exposure were complemented by an assessment of parameters serving as biomarkers for sublethal effects [such as total glutathione content (GSH), activities of the enzymes glutathione transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR)]. The data matrix was subjected to multivariate analysis to interpret relationships between different parameters and possible differences among locations. Results The multivariate statistical techniques helped to clearly identify the more contaminated upstream sites and separate them from the less contaminated and reference samples. The data document closer relationships of the detected sediment contamination with results of direct sediment exposure in the T. tubifex test regarding mortality but namely

  13. Assessment of hemato-biochemical parameters on exposure to low level of deltamethrin in mouse model

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    Anita Tewari

    2014-03-01

    Full Text Available Aim: In this study, sub-acute toxicity of deltamethrin on hematological and biochemical blood parameters of male albino Swiss mice was evaluated. Materials and Methods: Generally, the maximum permissible residue level (MRL of deltamethrin for food products lies between 0.01 to 0.5 mg/kg body weight. So the mice were exposed orally with two doses of pesticide i.e. 0.1 and 0.5 mg/kg body weight. The doses were given on a daily basis for a period of 15 days and 30 days respectively. Ground nut oil was used as control treatment. Samples of blood were collected at the end of the treatment. Hepatotoxicity was evaluated by quantitative analysis of the serum enzymes alanine transaminase (ALT, aspartate transaminase (AST, alkaline phosphatase (ALKP, total bilirubin (TBIL and serum urea. Alterations of hematological parameters were analysed by total leukocyte, differential leukocyte count and hemoglobin levels. Results: Significant increase in the levels of hepatic enzymes (ALT, AST, ALKP were observed for both doses, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations for 0.5 mg/kg body weight dose which is indicated by leukocytosis, lymphocytosis and neutropenia in long duration study. Conclusions: The results indicated that even very low dose of deltamethrin can promote hematological and hepatic alterations. Thus it is imperative to do further studies on the detrimental effect of the low levels of pyrethroid commonly present in our food, which further necessitate the reduction of maximum permissible levels of residual synthetic pyrethroid levels in foods and feed.

  14. Effect of Different Protein Levels On Nutrient Digestion Metabolism and Serum Biochemical Parameters in Calves

    Institute of Scientific and Technical Information of China (English)

    LI Hui; DIAO Qi-yu; ZHANG Nai-feng; TU Yan; WANG Ji-feng

    2008-01-01

    The current study has been performed to examine the effects of different dietary protein levels on the growth,nutrient digestion and absorption,as well as biochemical parameters in calves.Nine healthy newborn calves were selected,randomly divided into 3 groups and fed 3 milk replacers with different protein levels(18,22,and 26%),respectively.Five period-digestion-metabolism trials were taken between 12-20,22-30,32-40,42-50,and 52-60 days after birth.All 3 groups showed a similar growth curve during 11 to 61 experimental days,however,the growth rate of the 22%crude protein(CP) group was 8.89%higher than that of the 18%CP group and 19.48%higher than that of the 26%CP group.respectively. The apparent digestibility of dry material(DM)declined gradually with age,whereas,the apparent digestibility of N,extract ether(EE)rose slightly.Compared to the 22%CP and 26%CP group,calves fed with 18%CP apparently had a lower digestibility than DM,EE,and nitrogen(N).The average apparent digestibilities of N were 69.39,75.36,and 74.55%, respectively.Both the apparent digestibility and retention of calcium(Ca)and phosphorus(P)were steady throughout the experiment,but the average apparent digestibility of P in the 26%CP group was only 63.83%,which was markedly lower than that of the 18%CP group(70.40%)and 22%CP group(69.73%).In addition,the sernm concentrations of total protein(TP),albumin(ALB),and globulin(GLOB)of the 22%CP group were higher than those in the 18%CP and 26%CP groups.The urea N(BUN)content in the 18%CP group,on the other hand,was significantly lower than that of the other two groups.The highest glucose(GLU)concentration was found in the 22%CP group(5.38 mmol L-1),at the end of the trials.The protein levels in the milk replacer affected the digestion metabolism of nutrition and the serum biochemical parameters of calves at different physiological phases.Calves fed with 22%CP milk replacer had a better growth performance than the other groups.

  15. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    Directory of Open Access Journals (Sweden)

    R.N. Sani

    2016-06-01

    Full Text Available This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring, August and October (401 samples in summer. California Mastitis Test (CMT, milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial culture and CMT as were deemed to have glands with subclinical mastitis (SCM. The prevalence of subclinical mastitis in our study was around 12% (in one year. Of 144 (18.2% and 47 (11.72% positive samples in CMT, 134 (16.94% and 44 (10.97% samples were positive for bacterial culture in spring and summer, respectively. Comparison of results of hematological and serum biochemical parameters in ewes with SCM and healthy ewes in spring and summer showed that WBC counts, total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (P˂0.05. The results of the present study indicated that when interpreting hematological and serum biochemical parameters in ewes with SCM should be consider the effect of season on these parameters.

  16. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    Directory of Open Access Journals (Sweden)

    R.N. Sani

    2016-04-01

    Full Text Available This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring, August and October (401 samples in summer. California Mastitis Test (CMT, milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial culture and CMT as were deemed to have glands with subclinical mastitis (SCM. The prevalence of subclinical mastitis in our study was around 12% (in one year. Of 144 (18.2% and 47 (11.72% positive samples in CMT, 134 (16.94% and 44 (10.97% samples were positive for bacterial culture in spring and summer, respectively. Comparison of results of hematological and serum biochemical parameters in ewes with SCM and healthy ewes in spring and summer showed that WBC counts, total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (P˂0.05. The results of the present study indicated that when interpreting hematological and serum biochemical parameters in ewes with SCM should be consider the effect of season on these parameters.

  17. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA COLLECTED IN THE INACTIVE SEASON

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    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera in inactive season. The researches were carried out on honeybee samples collected from 5 honeybee colonies belonging to a breeding apiary of the Institute for Beekeeping Research and Development from Bucharest. In order to perform the biochemical analyses, the honeybees samples, consisting in 50 individuals on sample (10 individuals/colony were randomly collected and their haemolimph collected, at different time intervals, in inactive season (fall-winter. Totally, there were collected 250 haemolyph samples in a 2 years interval and the following 21 biochemical parameters were analysed: GLU, HDL-c, ALP, T-cho, Tprot, Alb., BUN, LDH, CPK,, Mg, IP, GGT, GOT, GPT, Ca, Cre,, Amy, T–BIL, TG, UA.. The test was carried out after the collection and processing of the samples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique, respectively .During the 2nd part of the inactive season, the values of most biochemical parameters increase in different proportions, their levels being maintained also in the first part of the active seasons (April, May, June.The values obtained for the main studied biochemical parameters in the haemolymph of the healthy honeybees collected from honeybee colonies kept in natural conditions show a highly variable evolution in the two consecutive years of experiments during the inactive season.

  18. Some Immunological, Hematological And Biochemical Parameters Characteristics Of The HCV Healthy Carrier

    International Nuclear Information System (INIS)

    The present study was aimed to identify some immunological, hematological and biochemical characteristics of the HCV healthy carrier state. Thirteen Egyptian HCV seropositive males who fulfilled the criteria of the HCV healthy carrier state were selected from 560 asymptomatic individuals. Fifteen age matched healthy HCV seronegative were used as control. Blood samples were withdrawn from all participants three times at six month interval to provide Leishmania stained smears, citrated plasma and sera. The following parameters were estimated: plasma prothrombin time and concentration, serum ALT and AST, L.E cells, hyper segmented neutrophils, cryo globulins, total IgM, albumin, C3, CIq immune complex and a-fetoprotein. Atypical lymphocyte and hyper segmented neutrophil proportion were determined from microscopical examination of Leishmania stained blood smears. Data from the present study showed that the HCV healthy carriers had significantly higher ALT, AST, prothrombin time, atypical lymphocytes and hyper segmented neutrophil proportion, serum C3, immune complex, IgM and a-fetoprotein than healthy HCV seronegative controls whereas serum albumin content did not change. In conclusion, the present data may suggest a role of the HCV in down regulation of the prothrombin synthesis and up regulation of the C3 production and immune complex formation and serum a-fetoprotein independently from hepatic damage. It also shows that atypical lymphocytes and hyper segmented neutrophils were increased in response to HCV infection

  19. Effect of gamma irradiation on ripening, storage and biochemical parameters of banana (Musa paradisiaca L.)

    International Nuclear Information System (INIS)

    Banana (Musa paradisiaca L.) popularly known as kadali (S) belongs to the family Musaceae is an economically important plant due to its nutritive fruit used as staple food all over the world. Several techniques have been employed to delay fruit ripening and softening by storing preclimacteric bananas in modified or controlled atmospheres. The Food and Drug Administration (FDA) has recommended the usage up to 1.0 kGy of gamma irradiation for delaying the ripening in fruits. Irradiation significantly improved shelf life by inhibiting the fungal growth in strawberries. Hence, present work was undertaken to scrutinize the impact of gamma irradiation on the ripening, storage and the biochemical attributes of Musa paradisiaca L. variety kadali. Present work revealed that irradiation at 0.2 kGy and 0.3 kGy retained their organoleptic properties till 16th day of storage against under normal conditions. Nutritional parameters such as ascorbic acid content, soluble sugar and protein contents were significantly higher in irradiated fruits. Hence, lower dose of gamma irradiation could be successfully used in extending the shelf life and nutritional quality and delay the process of ripening in Musa paradisiaca L.

  20. Comparison of biochemical parameters of rainbow trout (Oncorhynchus mykiss) reared in two different trout farms'

    Science.gov (United States)

    Karatas, Tayfun

    2016-04-01

    The aim of this study was to compare biochemical parameters of cultured rainbow trouts (Oncorhynchus mykiss, Walbaum, 1972) reared in two different trout farms' (Agri and Erzurum). The average weights of fish were 150±10gr for first station (Agri), 230±10gr for second station (Erzurum). Fishes used in research were randomly caught from pools, and fifteen pieces were used for each group. Fishes were fed with commercial trout feed with 45-50% crude protein twice a day. The levels of AST, ALT, LDL, total cholesterol and triglyceride in the second station (Erzurum) were found to be higher (p<0.05) than that of first station (Agri). Whereas, the levels of HDL in the second station (Erzurum) were found to be lower (p<0.05) than that of first station (Agri). Differences in the levels of total cholesterol and AST, ALT, HDL, LDL, triglyceride may be associated with size, sex, sexual maturity and environmental conditions (temperature, pH, hardness and dissolved oxygen).

  1. Protective role of wheat germ oil on some biochemical parameters in irradiated rats

    International Nuclear Information System (INIS)

    Wheat germ oil is an organic nutritionally rich vegetable oil. It is an excellent source of vitamin E and essential fatty acids (octacosanol, linoleic and linolenic). The study confirmed the beneficial role of the used anti-oxidation agents as recommended radio-protectors due to their ability of scavenging free radicals produced by ionizing radiation. The efficacy of daily oral treatment of rats with wheat germ oil (10 mg/ Kg body wt) for 15 days to control many of the damaging effects of ionizing radiation when male rats were subjected to fractionated 8 Gy (2 Gy day after day) of gamma irradiation were studied. Blood samples were collected from animals at 10 and 15 days after treatment and/or exposure. In blood, the data obtained revealed that, radiation exposure caused significant increases in levels of malondialdehyde (MDA), triacylglycerol (TG), total cholesterol (TC), low-density lipoproteins (LDL) and glucose at the 10th day. Meanwhile, significant reduction in contents of total protein, albumin, globulins, high-density lipoproteins (HDL), vitamin E and glucose were recorded at the 15th day. Also, the majority of these parameters were estimated in liver tissues. The results revealed that administration of the natural product wheat germ oil partially ameliorated the radiation-induced biochemical disturbances. These effects were explained in the light of the presumed different mechanisms of wheat germ oil actions

  2. Effect of Systemic Lupus Erythematosus on some Biochemical Parameters in Female Patients

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    Eman G.E Helal*, Medhat El- Shafaey**, Hala Rahoma

    2004-12-01

    Full Text Available It has been noticed that there is an increase in the number of women suffering from SLE. Most studies confirmed that serum DHEA (dehydroepiandrosterone and DHEA sulphate are lower among patients with SLE than among controls even- during phases of inactive disease so we performed this study to detect the level of DHEA-S in the female patients with SLE. The overall results confirm that DHEA treatment was well- tolerated, significantly reduced the number of SLE flares, and improved patient's global assessment of disease activity. Some serum parameters like liver and kidney functions were detected. Biochemical analysis showed that there is a significant increase of total lipids, cholestrol, triglycerides. But insignificant change in serum glucose, urea nitrogen and uric acid levels were recorded. From the present study three things were concluded. Firstly, there is a strong relationship between level of DHEA and the progression of SLE. Secondly, liver activity and kidney functions were not affected by SLE disease.Thirdly, DHEA treatment has a beneficial effect on female patients. So this study recommended to follow up DHEAS in female patients and use it in a proper dosage. In addition, further study must be done

  3. Effect of Gundelia tournefortii on some biochemical parameters in dexamethasone-induced hyperglycemic and hyperlipidemic mice

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    O. H. Azeez

    2012-01-01

    Full Text Available The aim of this study was conducted to evaluate the effect of Gundelia tournefortii on some biochemical parameters in hyperglycemic and hyperlipidemic mice. Male albino mice were induced hyperglycemic and hyperlipidemic by daily injection of dexamethasone 1 mg/kg of body weight intramuscularly (i.m., the mice randomly divided into five groups (6-8 mice in each group. The group 1: served as negative control group; the group 2: injected with dexamethasone at dose 1 mg /kg.b.w.i.m and served as positive control group; the groups 3, 4, 5: treated with extract of G. tournefortii at doses: 75, 150, 300 mg/kg.b.w. orally respectively companied with injection of dexamethasone 1 mg/kg.b.w.i.m. All treatment were once daily for 22 days. Dexamethasone treatment lead to significant increase in levels of glucose, cholesterol, and triglyceride, and significant decrease of body weight, without any effect on level of total protein. G. tournefortii extract treatment at doses: 75 mg/kg.b.w. resulted significant decrease levels of glucose, and body weight. Beneficial effect were seen when mice treated with G. tournefortii at dose of 300 mg/kg.b.w. that lead to significant decrease in levels of glucose, triglyceride, and cholesterol. These results indicate the usefulness of G. tournefortii extract as hypoglycemia and hypolipidemia in dexamethasone treated mice.

  4. Products of lipid peroxidation and selected biochemical and hematological parameters in plasma of patients with chronic pancreatitis

    Czech Academy of Sciences Publication Activity Database

    Podborská, Martina; Lojek, Antonín; Kubala, Lukáš; Ševčíková, A.; Trna, J.; Dítě, P.

    Brno, 2009. s. 140. ISSN 1337-6853. [TOXCON 2009, 14th Interdisciplinary Toxicology Conference. 01.06.2009-03.06.2009, Brno] R&D Projects: GA MŠk(CZ) OC08058 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : chronic pancreatitis * lipid peroxidation * biochemical and hematological parameters Subject RIV: BO - Biophysics

  5. Blood biochemical parameters of simmental cows in different phases of reproductive cycle

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    Radović Bisa

    2011-01-01

    Full Text Available This paper presents the values of blood biochemical parameters of Simmental cows (n = 60, divided into five groups (dry cows, early puerperium, late puerperium, the fifth month of lactation, and cows with an extended service period. The ration for lactating cows contained an insufficient amount of dry matter, a third less than the lower limit of their requirements. In all examined groups of cows, a strong protein deficiency and excess calcium was found in the diet. Total protein concentration in all groups of cows were on the upper physiological limit, and the concentrations of bilirubin, carotene, vitamin A and total lipids were within the normal range. Low concentrations of urea in the third, fourth and fifth group of cows (3.1±0.39 mmol/l, 2.9±0.38 mmol/l and 3.3±0.43 mmol/l, respectively indicate the imbalance of protein and energy in the diet. The presence of an energy deficit is also indicated by the low blood glucose in all groups of cows, especially the second and fifth groups of cows (2.2±0.04 mmol/l and 2.3±0.07 mmol/l, respectively. The unfavorable ratio of calcium and phosphorus in the diet resulted in the narrowing of the calcium:phosporus ratio in blood serum, which is particularly evident in cows with an extended service period (2.1±0.07 mmol/l compared to 1.9± 0.11 mmol/l. Based on these results,we conclude that the tested herd exhibits subclinical health disorders, caused by an inadequate diet and with adverse effects on the productive and reproductive parameters.

  6. Influence of Dietary Irradiated Curcuma ionga (Turmeric) on Physiological and Biochemical Parameters of Growing Rats

    International Nuclear Information System (INIS)

    Turmeric, (Curcuma ionga), is a dietary antioxidant and has been known since ancient times to possess therapeutic properties. The present investigation examined the correlation between raw and irradiated turmeric powder (at dose levels of 10, 15 and 20 kGy) intake and the physiological effects on organs weight, haematological parameters, indices of liver function and lipid profile in growing Albino male rats. Also, this study was performed to examine the efficacy of radiation processed turmeric powder to modulate the induced hyperlipidaemia in growing Albino male rats. Thirty six male rats were equally and randomly categorized into six groups. Control group fed a reference diet (casein diet), high fat diet was daily received to rats for 6 weeks. Other animals fed daily on high fat diets containing either raw or irradiated turmeric powder (2 g per 100 g diet) at doses 10, 15 or 20 kGy for 6 weeks. The results showed that the relative spleen, kidneys, heart, lungs, and testes weight, and the levels of Hb, PCV and MCHC were not changed in animals fed the experimental diets for 6 weeks. Animals kept on HFD suffered from liver enlargement. Dietary interventions through administrating 2% (w/w) raw or irradiated turmeric powder at 20 kGy normalized the liver size as compared with those received reference diet. Meanwhile, the results revealed that rats fed on high fat diet significantly increased plasma AST, ALT, TC and TG and significant decrease was observed in HDL. Meanwhile, feeding rats on diet containing of either raw or irradiated turmeric powder at 10, 15 and 20 kGy induced a significant improvement in the above mentioned parameters. These results imply that irradiated turmeric powder at 20 kGy can offer protection against the biochemical changes as a consequence of hyperlipidaemic food and contribute to the regulation of lipid metabolism safely

  7. Practical identifiability of the manipulator link stiffness parameters

    OpenAIRE

    Klimchik, Alexandr; Caro, Stéphane; Pashkevich, Anatol

    2013-01-01

    The paper addresses a problem of the manipulator stiffness modeling, which is extremely important for the precise manufacturing of contemporary aeronautic materials where the machining force causes significant compliance errors in the robot end-effector position. The main contributions are in the area of the elastostatic parameters identification. Particular attention is paid to the practical identifiability of the model parameters, which completely differs from the theoretical one that relie...

  8. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation

    International Nuclear Information System (INIS)

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  9. INFLUENCE OF PULSED SHORT WAVE (DIAPULSE TREATMENT ON THE BIOCHEMICAL PARAMETERS CORTISOL AND ALDOSTERONE

    Directory of Open Access Journals (Sweden)

    Dogaru Gabriela

    2015-12-01

    Full Text Available Pulsed short waves are part of high frequency therapy; unlike continuous short waves, where the phenomenon of tissular endothermia occurs, they have therapeutic effects that seem to depend rather on the interaction of electromagnetic waves with biological tissue, as they act only through specific electromagnetic energy. The recommended work technique for the use of the Diapulse device is that regardless of the exposed somatic region, there will be an initial exposure of one of the following regions: prehepatic, epigastric or lumbar (for action on the adrenal glands. Considering the role and the effects of the hormones of the adrenocortical gland, particularly glucocorticoids, on the body, which influence the inflammatory response of the connective tissue, with a role in rheumatoid pathology, we monitored the changes in the function of this gland. The biochemical parameters cortisol and aldosterone were quantitatively assessed in patients with complex regional pain syndrome type I (algoneurodystrophy treated with pulsed short waves, who were initially exposed to radiation of the lumbar region. In the same treatment session, all patients were exposed to radiation of the lumbar region, at a dose of 4/400 impulses/sec., for 10 minutes, followed by radiation of the affected region at a dose of 6/600 impulses/sec., for 10 minutes. There was one treatment session per day, for 14 days. It was demonstrated that radiation of the lumbar region at a dose of 4/400 impulses/sec. caused no changes in the hormonal function of the adrenal gland, i.e. no hypo- or hyperfunction; there was no overstrain or overstress after exposure to pulsed short waves, which is an important aspect, with applicability in clinical practice. Studies demonstrated both the local and the general, systemic influence of pulsed short waves, the induced changes being for the most part strictly dependent on the dose used.

  10. Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna.

    Science.gov (United States)

    Bownik, Adam; Stępniewska, Zofia; Skowroński, Tadeusz

    2015-02-01

    Ectoine (ECT) is a compatible solute produced by soil, marine and freshwater bacteria in response to stressful factors. The purpose of our study was to determine the possible toxic influence of ECT on Daphnia magna. We determined the following endpoints: survival rate during exposure and recovery, swimming performance, heart rate, thoracic limb movement determined by image analysis, haemoglobin level by ELISA assay, catalase and nitric oxide species (NOx) by spectrophotometric methods. The results showed 80% survival of daphnids exposed to 50mg/L of ECT after 24h and 10% after 90h, however lower concentrations of ECT were well tolerated. A concentration-dependent reduction of swimming velocity was noted at 24 and 48h of the exposure. ECT (at 2.5 and 4mg/L) induced an increase of heart rate and thoracic limb movement (at 2.5, 4 and 20mg/L) after 24h. After 10h of the exposure to ECT daphnids showed a concentration-dependent increase of haemoglobin level synthesized and accumulated in the epipodite epithelia. After 24h we noted a concentration-dependent decrease of haemoglobin level and its lowest value was found after 48h of the exposure. ECT at a concentration of 20 and 25mg/L slightly stimulated catalase activity after 24h. NOx level was also increased after 10h of the exposure to 20 and 25mg/L of ECT reaching maximal activity after 24h. Our results suggest that ECT possesses some modulatory potential on the behaviour, physiology and biochemical parameters in daphnids. PMID:25460046

  11. The effects of Ramadan fasting and physical activity on body composition and hematological biochemical parameters

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    Seyyed Reza Attarzadeh Hosseini

    2014-09-01

    Full Text Available Introduction: Hunger and reduction in regular energy intake can lead to a number of problems based on their intensity. For instance, low energy level can cause blood cell production to decline or it may pose a higher risk of anemia. It can also weaken the immune system and platelet aggregation or negatively affect clot formation. This study aimed to have a closer look at fasting and regular physical activity and their impacts on body composition and blood hematological-biochemical parameters among professional wrestlers. Method: In this semi-experimental study, 9 subjects were selected by convenience sampling. The selected training program included participation in this exerciseprogram, 90 min per session, 6 times per week for a period of one month. Blood samples were obtained four times: before the start of Ramadan, 2 weeks after the start, during the last week and 2 weeks after the end of Ramadan. To make intra-group comparison, repeated measure analysis of variance was used. For all statistical comparisons, the level of significance was considered at PResults: Body weight and red blood cell count (RBC dropped significantly at the end of Ramadan (Respectively P= 0.001 and P=0.034. However, the number of white blood cells (WBC and circulating platelets (PLT significantly increased during fasting (Respectively P= 0.048 and P=0.042. As a matter of fact, PLT and WBC were the only factors which dramatically increased during fasting. Intra-group variations of tetracycline (TC, low-density lipoprotein (LDL, LDL: high-density lipoprotein (HDL, triglyceride (TG: HDL and TC: HDL reduced at the end of Ramadan. However, HDL levels ​​drastically increased during fasting (P≤0.05. Conclusion: Based on the results of the research, despite being a regular activity and fasting has beneficial effects on lipid profile in athletes, however, they can with tangible changes in hematological factors may lead to weaken the immune system of athletes.

  12. Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites.

    Science.gov (United States)

    Wang, Yongwei; Guo, Yuming; Ning, Dong; Peng, Yunzhi; Cai, Hong; Tan, Jianzhuang; Yang, Ying; Liu, Dan

    2012-01-01

    Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC), hematocrit (HCT) and haemoglobin (HGB) of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA) level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD) was significantly decreased. Hepatic lactic acid (LD) level of ascitic broilers were significantly lower than healthy ones. Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG) and blood urea nitrogen (BUN) level. The activity of liver hexokinase (HK) and succinodehydrogenase (SDH) in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK) and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1) cytoskeleton; 2) glucose, lipids and amino acid metabolism; 3) cell secretion; 4) cell apoptosis; 5) signal transduction; 6) immune and inflammatory response; and 7) cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK) mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated, but

  13. Effects of D2O on biochemical parameters of normal cells and tumour cells

    International Nuclear Information System (INIS)

    The influence of high temperatures (Hyperthermia) on normal tissue and Ehrlich-Ascites tumour cells ('ATZ') was examined under several conditions with regard to the application of deuterium oxide as a stabilising factor. It was proven that the DNA-synthesis of normal tissue (liver, mouse) is not sensitive to temperature. This effect of hyperthermia only occurs when the tissue is damaged, e.g. by trypsinising. The influence of hyperthermia on several biochemical parameters and on morphological changes of the Ascites cells was examined. The findings show that deuterium oxide (D2O) is able to reduce both the thermal and the ureal denaturation of enzymes. Thus tests were carried out to find out if D2O also reduces toxic influence in complicated biological systems. The assumption of high D2O concentrations to prevent several reactions was confirmed. When the Ascites tumour cells in the H2O-buffer were exposed to the damaging influence of hyperthermia, the high degree of damage was seen with the decreasing DNA synthesis, reduced aerobic glycose capacity, a drop in the ATP values and breakdown of the permeability of the membrane. Deuterium oxide was able under high temperature (from appr. 440C on) to reduce the degree of damage to DNA synthesis, while auto-effects (inhibition of synthesis) of D2O predominate in the lower region. Aerobic glycolysis was damaged in both cases to the same degree, however. In D2O after hyperthermia the ATP-level dropped faster than in H2O. D2O not only reduces the thermal denaturation of the Ascites tumour cells, but it also eliminates the toxic influence of the zytostaticum TRENIMONsup(R) (under 380 or 460C incubation). (orig./AJ)

  14. Effect Of Some Herbal Medicine On Some biochemical parameters In Diabetic Rats

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    Eman G.E. Helal, Mervat A.Abbas

    2006-03-01

    Full Text Available The current study was planned to follow up the effect of famous mixture used as a hypoglycemic traditional medicine in Saudi Arabia on diabetic albino rats and their effects on some biochemical parameter as DHEA .This study also aimed to study the effect of each plants alone to illustrate the most powerful one of them. The present study was carried out on eighty adult male albino rats (120+20gm b.wt. They were randomly divided into eight groups. First group conserved as control group.The others groups received alloxan to become diabetic .The second group conserved as diabetic, the third group treated with a mixture of plants (0.5gm/120gm b.wt.From he fourth to eight group,the rats treated with Nigella sativa (0.25gm/120gm b.wt,Aloe vera,Ferulaassa-foetid,Boswellia Carterii Birdw and Commiphora myrrha respectively (0.5gm/120gm b.wt..Our result showed that the body weight was reduced significantly in diabetic group and Nigella sativa treated group. On the other hand, treatment with commiphora myrrha revealed significant increase in body weight gain, while mixture and Aloe vera treated rats recorded insignificant change. Diabetic rats revealed a significant decrease in serum glucose and DHEA-S levels and liver glycogen content, while insignificant difference was recoded in all treated groups. A significant decrease in serum insulin level was observed in diabetic group, Nigella sativa and Ferula assa-foetida treated groups, but insignificant different was recorded in the rest treated groups throughout the experiment. A significant increase in LDH activities in diabetic and Ferula assa-foetida treated groups was recorded while rest treated groups recorded insignificant change During experimental period.

  15. Lymphocytic Thyroiditis – is cytological grading significant? A correlation of grades with clinical, biochemical, ltrasonographic and radionuclide parameters

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    Dash Radharaman

    2007-01-01

    Full Text Available Abstract Background Clinical, biochemical, ultrasonographic, radionuclide and cytomorphological observations in Lymphocytic thyroiditis (LT, to define the cytological grading criteria on smears and correlation of grades with above parameters. Methods This prospective study was conducted on 76 patients attending the Fine needle aspiration cytology clinic of a tertiary care institute in North India. The various parameters like patients' clinical presentation, thyroid antimicrosomal antibodies, hormonal profiles, radionuclide thyroid scan and thyroid ultrasound were studied. Fine needle aspiration of thyroid gland and grading of thyroiditis was done on smears. The grades were correlated with above parameters and the correlation indices were evaluated statistically. Results Most of the patients were females (70, 92.11% who presented with a diffuse goiter (68, 89.47%. Hypothyroid features (56, 73.68% and elevated TSH (75, 98.68% were common, but radioiodide uptake was low or normal in majority of patients. Thyroid antimicrosomal antibody was elevated in 46/70 (65.71% patients. Cytomorphology in fine needle aspirates was diagnostic of lymphocytic thyroiditis in 75 (98.68% patients. Most of them had grade I/II disease by cytology. No correlation was observed between grades of cytomorphology and clinical, biochemical, ultrasonographic and radionuclide parameters. Conclusion Despite the availability of several tests for diagnosis of LT, FNAC remains the gold standard. The grades of thyroiditis at cytology however do not correlate with clinical, biochemical, radionuclide and ultrasonographic parameters.

  16. Assessing seasonality of biochemical CO2 exchange model parameters from micrometeorological flux observations at boreal coniferous forest

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    T. Vesala

    2008-12-01

    Full Text Available The seasonality of the NEE of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the biochemical model parameters. Our study sites consisted of three Scots pine (l. Pinus sylvestris forests and one Norway spruce (l. Picea abies forest that were located in Finland and Sweden. We obtained temperature and seasonal dependence for the biochemical model parameters: the maximum rate of carboxylation (Vc(max and the maximum rate of electron transport (Jmax. Both of the parameters were optimized without assumptions about their mutual magnitude. The values obtained for the biochemical model parameters were similar at all the sites during summer time. To describe seasonality, different temperature fits were made for the spring, summer and autumn periods. During summer, average Jmax across the sites was 54.0 μmol m−2 s−1 (variance 31.2 μmol m−2 s−1 and Vc(max was 12.0 μmol m−2 s−1 (variance 6.6 μmol m−2 s−1 at 17°C. The sensitivity of the model to LAI and atmospheric soil water stress was also studied. The impact of seasonality on annual GPP was 17% when only summertime parameterization was used throughout the year compared to seasonally changing parameterizations.

  17. Hierarchical clustering of 54 races and strains of the mulberry silkworm, Bombyx mori L: Significance of biochemical parameters.

    Science.gov (United States)

    Chatterjee, S N; Datta, R K

    1992-12-01

    A detailed analysis was undertaken to test the efficacy of hierarchical agglomerative clustering (UPGMA method) in grouping the races and strains of the mulberry silkworm, Bombyx moti L., and to ascertain the importance of biochemical parameters in the clustering process. The analysis was based on data from two rearing seasons with 54 selected races/strains of different geographic origin and varying yield potentials. The results indicate that seven clusters can be realised with yield parameters alone, whereas the inclusion of biochemical parameters in clustering resulted into two broad groups: one having all the breeds with high cocoon weight and shell weight, the other having all the low-yielding silkworm strains both from India and from other countries. Further sub-grouping under these two groups highlights genetical differences associated with the differentiation of various groups of races in temperate and tropical areas as well as their significance for silkworm breeding. Estimates of all ten variables were further subjected to 'quick clustering' and the results showed that cluster 5, constituted by 38 lowyielding strains of India, China and Europe, had the highest values of the final cluster centre for amylase and the effective rate of rearing (ERR), while clusters 1 and 4 had the highest values for invertase and alkaline phosphatase. The evolutionary aspect of the genetic channelisation of silkworm races from various countries is discussed against the background of differences in the biochemical parameters and yield variables. PMID:24197452

  18. Effect of parenterally administered cystamine and gammaphos (WR-2721) on some biochemical parameters in dog blood serum

    International Nuclear Information System (INIS)

    The effects were studied of intravenous and intramuscular administration of radioprotectives cystamine and gammaphos in dogs on the biochemical parameters of the blood serum. The activities were studied of enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine kinase, gamma-glutamyl transpeptidase, lactate dehydrogenase, malate dehydrogenase, sorbitol dehydrogenase and alpha-amylase. The contents were determined of total protein, albumin, bilirubin, urea nitrogen, creatinine, glucose, triglycerides, cholesterol, lipids, calcium, sodium and potassium. Cystamine was shown to be hepatotoxic. The intramuscular administration of gammaphos was found to be more advantageous than of cystamine. Only slight increase was observed in the activities of lactate dehydrogenase, malate dehydrogenase, and alpha-amylase. With cystamine, the changes in all biochemical parameters were most marked. (M.D.). 17 figs., 18 refs

  19. The effects of salt stress on growth and biochemical parameters in two maize varieties

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    Elżbieta Sacała

    2014-02-01

    Full Text Available The main objective of this study was to examine the influence of salinity on growth and biochemical parameters (NR activity, amino compound accumulation, protein content, some inorganic ion concentrations of two varieties of maize (Limko and Koka. Salinity (75.0 mol • m-3 NaCl and 37.5 mol • m-3 Na2SO4 significantly reduced fresh and dry weights of the investigated maize varieties. NaCl induced higher reduction in biomass production than Na2SO4. Differences in salt tolerance between Limko and Koka were small.It was found that in both maize varieties the nitrate reductase activity decreased under salt stress (100 mol • m-3 NaCl. This effect was more marked in the Koka variety than Limko. Decrease in nitrate reductase activity had no inhibitory effect on accumulation of protein and free amino compounds. Salinity treatment (100 mol • m-3 NaCl led to an increase in free amino compound contents in roots and shoots of both investigated maize varieties, but these changes were not very large. The highest increase in amino compound level was observed in roots of Koka and it was 2-fold higher than in control plants. Salt stress did not change soluble protein contents in Limko and Koka varieties with the exception of Limko leaves. There was significant increase in soluble protein content on leaves of Limko and it amounted 128% of the control. Salinity caused a great increase in leaf and root Na+ concentration and a decrease in case of Ca2+ and K+ contents. The declines in Ca2+ content in maize roots were 76 and 70% respectively for Koka and Limko roots. The fall in K+ concentration was high, but not so drastic as in Ca2+. In roots of Koka and Limko the reduction in K+ content was approximately 30%. Higher reduction in K+content showed shoots of Limko. In this case K+ content lowered to 49% of control.

  20. Effects of Race Distance on Physical, Hematological and Biochemical Parameters of Endurance Horses

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    Lawan Adamu

    2010-01-01

    Full Text Available Problem statement: Endurance horses are generally exposed to severe stress during endurance competitions. These horses are however conditioned to cover the long distances of the competitions at moderate speeds. Approach: This study was conducted in Malaysia to determine the post-race hematological, blood electrolyte and biochemical and physical parameters of horses of 40, 80 and 120 km endurance race categories and eliminated and horses that completed the races with good performance. Whole blood, plasma and serum samples were collected after each race. Results: After physical examination 7 (9.72% 120 km-, 48 (66.67% 80 km- and 17 (23.61% 40 km-race horses were eliminated. Eight horses, all from the 80 km-race category completed the race with good performance. The mean heart of the good performance horses (74.2±13.9 beats min-1 was higher than that of the eliminated 40 km- (68.2±14.7 beats min-1, 80 km-race (62.9±9.7 beats min-1 horses. The blood lactate concentration of good performance horses was 9.2±2.2 mmol L-1, which was significantly higher than in the 40 km- (6.0±2.9 mmol L-1, 80 km- (6.7±3.2 mmol L-1 and the 120 km-race (6.4±1.6 mmol L-1 horses. The blood glucose concentration was lower in the good performance horses (1.6±0.9 mmol L-1 than the eliminated 40 km- (5.7±1.9 mmol L-1, 80 km- (4.3±2.4 mmol L-1 and the 120 km-race (5.0±1.4 mmol L-1 horses. Conclusion: The study showed that eliminated horses exhibited poorer glucose utilization than good performance horses, which may have resulted in poor lactate production. Thus the blood lactate and glucose concentrations of horses during training may be used to predict their performance in endurance races.

  1. Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites

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    Wang Yongwei

    2012-12-01

    Full Text Available Abstract Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS. Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC, hematocrit (HCT and haemoglobin (HGB of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD was significantly decreased. Hepatic lactic acid (LD level of ascitic broilers were significantly lower than healthy ones. Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG and blood urea nitrogen (BUN level. The activity of liver hexokinase (HK and succinodehydrogenase (SDH in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1 cytoskeleton; 2 glucose, lipids and amino acid metabolism; 3 cell secretion; 4 cell apoptosis; 5 signal transduction; 6 immune and inflammatory response; and 7 cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated

  2. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation

    OpenAIRE

    Mishra, Arun K.; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with s...

  3. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries

    OpenAIRE

    A. Kaur; K S Kwatra; Kamboj, P.

    2012-01-01

    Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free ...

  4. Effects of Operating Parameters on Measurements of Biochemical Oxygen Demand Using a Mediatorless Microbial Fuel Cell Biosensor

    OpenAIRE

    Min-Chi Hsieh; Chiu-Yu Cheng; Man-Hai Liu; Ying-Chien Chung

    2015-01-01

    The conventional Biochemical Oxygen Demand (BOD) method takes five days to analyze samples. A microbial fuel cell (MFC) may be an alternate tool for rapid BOD determination in water. However, a MFC biosensor for continuous BOD measurements of water samples is still unavailable. In this study, a MFC biosensor inoculated with known mixed cultures was used to determine the BOD concentration. Effects of important parameters on establishing a calibration curve between the BOD concentration and out...

  5. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

    OpenAIRE

    Mirjana Joksimović Todorović; Vesna Davidović

    2012-01-01

    The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA), activity of enzymes of aspartat aminotransferase (AST), alanin aminotransferase (ALT) and gamma glutamyltranferase (GGT), in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartu...

  6. Hematological And Biochemical Parameters In Elite Soccer Players During A Competitive Half Season

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    Anđelković Marija

    2015-10-01

    Full Text Available Background: The purpose of the present study was to report and discuss the hematological and biochemical behavior of elite soccer players, in order to get more insight in the physiological characteristics of these sportsmen and to provide trainers and sports doctors with useful indicators.

  7. The Effects of Ramadan Fasting and Physical Activity on Blood Hematological-Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    2013-07-01

    Fasting during the month of Ramadan by regular physical activity caused positive alterations in Hematological-Biochemical Index. These changes may be due to the alterations in diet, biology response of the body to the starving and physical activity during this month.

  8. Biochemical parameters of bone metabolism in bone metastases of solid tumors (Review)

    NARCIS (Netherlands)

    Meijer, Wilhelmus; van der Veer, E; Willemse, P H

    1998-01-01

    The role of biochemical markers of bone metabolism in the diagnosis and monitoring of bone metastases in solid tumors is reviewed. Emphasis is on the recently developed markers, which may provide a more accurate quantitation of bone metabolism. In metastatic bone disease, bone formation and resorpti

  9. Influence of anti-epileptic drugs on hematological and biochemical parameters in patients with epilepsy

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    S. Dwajani

    2014-08-01

    Conclusions: The results of this study showed significant alterations in the levels of Hb and ALP with the use of AED polytherapy in PWE. Routine hematological and biochemical investigations may be considered during AED treatment in those patients receiving AED polytherapy. [Int J Basic Clin Pharmacol 2014; 3(4.000: 692-695

  10. The Association between some Incidental Factors and Biochemical Parameters Linked to Atherogenesis

    International Nuclear Information System (INIS)

    The present study was conducted to assess the role played by some factors including age, residence location, diet, hypertension and job pattern, as incidental factors in the atherogenic process, on the lipid, lipoprotein, homocysteine, vitamin B 12 and folic acid in apparently healthy Egyptian males. Sera from one hundred and forty six apparently healthy male volunteers were analyzed for total cholesterol, triglycerides, high density Iipoproteins cholesterol (HDL-c), low density lipoproteins cholesterol (LDL-c), apolipoprotein A. (Apo At), apolipoprotein B ( Apo B ) and homocysteine, in addition to vitamin BI2 and folic acid. Data from the present study showed that middle aged individuals (over 40 years), urban volunteers, butter users, volunteers experiencing mental work and hypertensives experienced increased lipid fractions when compared to their counterparts. The data also showed the lack of an effect of the previous factors on serum homocysteine, folic acid or vitamin B12. In view of the previous findings, it may be concluded that factors such as: age, urban residence, butter consumption, hypertension and intellectual work may contribute to the etiology of atherosclerosis through their impact on serum lipid fractions. Serum homocysteine and related vitamins B12 and folic acid seem to be unmodified by these factors

  11. Effects Of Music Therapy On Clinical And Biochemical Parameters Of Metabolic Syndrome

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    Rajnee

    2011-12-01

    Full Text Available Background: Music therapy is a new approach being used for the management of metabolic abnormalities and stress related illness.Objective: To study the effect of Music therapy on various clinical and biochemical parameters of Metabolic Syndrome.Methods: This cross sectional study was carried out on 100 patients of metabolic syndrome selected randomly. These patients were divided into two equal groups after age, sex adjustment. In control group (group I 50 patients underwent the conventional treatment. 50 patients in study group were treated with supervised music protocol along with conventional treatment. The Body Mass Index, ;Waist-Hip ratio, Blood pressure, Fasting blood sugar were monitored weekly while HbA1c and lipid profile were determined at the baseline and after three months of exposure to music therapy. Statistical analysis was performed by employing student t- test.Results: In the study group there was a significant decrease in BMI (27.18±5.02 to 25.44±3.49 kg/m2, p<0.05, waist hip ratio (0.95±0.05 to 0.93±0.05 cm, p<0.05, Fasting blood sugar (196.00±47.80mg/ dl to152.00±16.19mg/dl , p<0.001, HbA1c (8.41±1.31% to 7.08±0.78 % p<0.001, Systolic Blood Pressure (151.00±12.10 to 136±9.04 mmHg p<0.001, Diastolic Blood Pressure (94±4.80 to 86.44±3.16 mmHg, p<0.01, Mean serum cholesterol (257.80±18.92 to 229.12±17.82mg/dl, p<0.001 and triglycerides (180.86±14.04 to 136.50±8.92mg/dl, p<0.001, LDL (167.97±14.40 to 140.20±15.41mg/dl, p<0.001, and VLDL (33.60±2.88 to 28.04±3.08mg/dl, p<0.001 and increase in HDL (33.32±3.38 to 39.71±3.41mg/dl, p<0.001, when compared with those of control group not receiving the music therapy along with the conventional treatment.Conclusion: The promising outcomes of Music therapy showed that it may be considered as a useful adjunct to conventional treatment in management of the metabolic syndrome. This study advocates music therapy to establish it from a general well being concepts to a

  12. Evaluation of the effect of conventionally prepared swarna makshika bhasma on different bio-chemical parameters in experimental animals

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    Sudhaldev Mohapatra

    2011-01-01

    Full Text Available Swarna makshika (chalcopyrite bhasma (SMB has been used for different therapeutic purposes since long in Ayurveda. The present study is conducted to evaluate the effect of conventionally prepared SMB on different bio-chemical parameters in experimental animals, for providing scientific data base for its logical use in clinical practice. The genuine SMB was prepared by following classical techniques of shodhana and marana most commonly used by different Ayurvedic drug manufacturers. Shodhana was done by roasting raw swarna makshika with lemon juice for three days and marana was performed by 11 putas . The experimental animals (rats were divided into two groups. SMB mixed with diluted honey was administered orally in therapeutic dose to Group SMB and diluted honey only was administered to vehicle control Group, for 30 days. The blood samples were collected twice, after 15 days and after 30 days of drug administration and different biochemical investigations were done. Biochemical parameters were chosen based on references from Ayurvedic classics and contemporary medicine. It was observed that Hb% was found significantly increased and LDL and VLDL were found significantly decreased in Group SMB when compared with vehicle control group. This experimental data will help the clinician for the logical use of SMB in different disease conditions with findings like low Hb% and high LDL, VLDL levels.

  13. Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids

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    Mladenović Jelena M.

    2014-01-01

    Full Text Available The effects of subchronic exposure to copper (Cu on lipid peroxidation, hemato-biochemical parameters, and the possible protective role of flavonoids Quercetin and (--Epicatechin were studied. Male Wistar albino rats were treated with Cu (560 mg/L, p.o. as CuCl2•2H2O for 5 weeks and Quercetin and (--Epicatechin (40 mg/kg BW each, i.p., every third day during the last 3 weeks alone or in combination. Cu increased the concentration of lipid peroxides, decreased the number of erythrocytes, hemoglobin and hematocrit values and increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase. Coadministration of Quercetin and (--Epicatechin with Cu lowered the process of lipid peroxidation and restored examined hemato-biochemical parameters to control values. Our results indicate that Cu induced oxidative damage in erythrocytes, which led to anemia, while Quercetin and (--Epicatechin showed a protective effect on the hemato-biochemical processes in the blood of rats. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  14. Biochemical Engineering Parameters for Hydrometallurgical Processes: Steps towards a Deeper Understanding

    OpenAIRE

    K. Kundu; Kumar, A.

    2014-01-01

    Increasing interest in biomining process and the demand for better performance of the process has led to a new insight toward the mining technologies. From an engineering point of view, the complex network of biochemical reactions encompassed in biomining would best be performed in reactors which allow a good control of the significant variables, resulting in a better performance. The subprocesses are in equilibrium when the rate of particular metal ion; for example, iron turnover between the...

  15. Spent Mushroom Substrate Influences Elk (Cervus Elaphus Canadensis) Hematological and Serum Biochemical Parameters

    OpenAIRE

    Park, Jae Hong; Kim, Sang Woo; DO, YOON JUNG; Kim, Hyun; Ko, Yeoung Gyu; Yang, Boh Suk; Shin, Daekeun; Cho, Young Moo

    2012-01-01

    The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p

  16. ESTIMATING BIOCHEMICAL PARAMETERS OF TEA (CAMELLIA SINENSIS (L.)) USING HYPERSPECTRAL TECHNIQUES

    OpenAIRE

    Bian, M.; Skidmore, A.K.; Schlerf, M.; Liu, Y; Wang, T.

    2012-01-01

    Tea (Camellia Sinensis (L.)) is an important economic crop and the market price of tea depends largely on its quality. This research aims to explore the potential of hyperspectral remote sensing on predicting the concentration of biochemical components, namely total tea polyphenols, as indicators of tea quality at canopy scale. Experiments were carried out for tea plants growing in the field and greenhouse. Partial least squares regression (PLSR), which has proven to be the one of th...

  17. Effect of the sunlight on biochemical and physiological parameters of animal

    International Nuclear Information System (INIS)

    Animal perception of the sunlight spectrum with 450, 540, 680 nm wave lengths and the same intensity has been studied. The effect of a sunlight action on rats has been evaluated from some functional and biochemical factors both in vivo and in vitro. A totality of the results obtained allows one to make an assumption that a sunlight energy redistribution in animal tissues as a function of spectral composition of incident light plays a leading role in metabolism regulation

  18. Alteration of biochemical parameters after supplementation of multivitamins and minerals of sprayers on grape gardens of Western Maharashtra (India

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    Jyotsna A. Patil

    2014-01-01

    Full Text Available Background: In the horticulture sector, the land used for cash crop like grapes is increasing particularly in Maharashtra state. There is an increasing use of the pesticides in an attempt to increase the yield and reduce the post harvest losses. The environmental pollution and poisoning due to wide use of pesticide during grape cultivation may be much more important factors to disturb the socio economical status of uneducated farm worker in rural areas. Aim and Objectives: The study aimed to evaluate the pesticide exposure of the population associated with grape cultivation with respect to biochemical effects, oxidative stress, antioxidants status and protecting them from such effects by supplementing vitamins A to Z. Material and Methods: For this study 30 subjects with occupational pesticides exposure i.e. sprayers of grape gardens having age in the range of 20 to 45 years from Tasgaon taluka, Sangli district, (Western Maharashtra India were taken and all these study group subject,500 mg vitamin A to Z tablet / day for fifteen days were supplemented. The blood was collected by venipuncture from all these subjects for biochemical parameters assay before and after fifteen days of vitamins A to Z supplementation and all biochemical parameters were estimated by using standard methods. Results: Fifteen days vitamin A to Z and multi minerals supplementation to sprayers of grape gardens, we observed significant increase in serum Acetyl Cholinesterase (P<0.001, 2.32%, and decreased Aspartate Transaminase (P<0.05, 8.36%, Alanine Transaminase (P<0.05, 14.16%, serum Lipid Peroxide (P<0.001, 24.39%, Glutathione S-Transferase (P<0.01, 29.84% and increased RBC-Superoxide Dismutase (P<0.05, 11.55%, RBC-Catalase (P<0.001, 25.7%, serum zinc (P<0.01, 3.74%, copper (P<0.001, 4.13%, blood lead (P<0.01, 3.33% as compared to before vitamin A to Z and multi minerals supplementation and other biochemical parameters such as serum C-Reactive Proteins, Total Proteins

  19. Effects of cadmium at sub-lethal concentration on growth and biochemical parameters in rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Heydarnejad, M Saeed; Khosravian-Hemamai, Mozhdeh; Nematollahi, Amin

    2013-01-01

    Cadmium (Cd), as one of heavy metals and an environmental stressor, may alter many physiological processes like growth and serum parameters in fish. The main objective of this study was to determine the effects of cadmium at sub-lethal concentrations (1 and 3 μg/l) on growth and serum biochemical parameters including enzymes, i.e. alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), glucose, triglyceride, cholesterol and total protein in rainbow trout (Oncorhynchus mykiss). Trout were exposed to cadmium, and, at intervals of 1, 15, and 30 days, selected parameters were evaluated. Condition Factor (K), Specific Growth Rate (SGR) and Body Weight Gain (BWG) consistently decreased, while Food Conversion Ratio (FCR) increased at the end of experiment. Glucose was elevated in trout exposed to both Cd concentrations at day 15 and then returned to levels comparable to control fish. Triglyceride and cholesterol decreased transiently at day 15 and then increased at day 30. Total protein, AST, ALT and ALP increased linearly by time and Cd concentration. This investigation suggests that growth and serum biochemical parameters could be used as important and sensitive biomarkers in ecotoxicological studies concerning the effects of metal contamination and fish health. PMID:23782857

  20. Impact of gamma irradiation on biochemical parameters of Flacourtia Montana Grahm during different storage period

    International Nuclear Information System (INIS)

    Biochemical attributes such as ascorbic acid content, anthocyanin content, PAL activity, phenolics content and antioxidant activity of the irradiated (0-2 kGy) F. montana Grahm. fruits were determined during 4 weeks of cold storage. Doses of 1.5 to 2 kGy enhanced the ascorbic acid content where as total anthocyanin was maintained at 1.5 kGy. Increase in PAL activity was observed with radiation treatment which may count for increased phenolics. A significant increase in the DPPH radical scavenging and reducing power was observed at 0.5 kGy, 1 kGy and 2 kGy. (author)

  1. Protein coalitions in a core mammalian biochemical network linked by rapidly evolving proteins

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    Tsoka Sophia

    2011-05-01

    Full Text Available Abstract Background Cellular ATP levels are generated by glucose-stimulated mitochondrial metabolism and determine metabolic responses, such as glucose-stimulated insulin secretion (GSIS from the β-cells of pancreatic islets. We describe an analysis of the evolutionary processes affecting the core enzymes involved in glucose-stimulated insulin secretion in mammals. The proteins involved in this system belong to ancient enzymatic pathways: glycolysis, the TCA cycle and oxidative phosphorylation. Results We identify two sets of proteins, or protein coalitions, in this group of 77 enzymes with distinct evolutionary patterns. Members of the glycolysis, TCA cycle, metabolite transport, pyruvate and NADH shuttles have low rates of protein sequence evolution, as inferred from a human-mouse comparison, and relatively high rates of evolutionary gene duplication. Respiratory chain and glutathione pathway proteins evolve faster, exhibiting lower rates of gene duplication. A small number of proteins in the system evolve significantly faster than co-pathway members and may serve as rapidly evolving adapters, linking groups of co-evolving genes. Conclusions Our results provide insights into the evolution of the involved proteins. We find evidence for two coalitions of proteins and the role of co-adaptation in protein evolution is identified and could be used in future research within a functional context.

  2. THE EFFECT OF THE KETOGENIC DIET ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF THE CHILDREN WITH RESISTANT EPILEPSY

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    Mohammadreza ALAEI

    2010-04-01

    Full Text Available ObjectiveThe aim of this study was to evaluate the effect of the ketogenic diet on the growth parameters of the children with resistant epilepsy.Materials & MethodsA total of 36 children with resistant epilepsy who were 2 to 7 year old were put on the ketogenic diet. Their growth and biochemical parameters were studied at the beginning of the study and after 3 months.ResultsWeight decreased in all patients. Serum levels of hemoglobin, calcium, and blood sugar decreased significantly but remained in the normal range. Creatinine did not change, but BUN showed a significant increase.ConclusionWe can lower the complications of ketogenic diets by using more unsaturated fat, more water, and more minerals.Keywords: ketogenic diet, epilepsy, growth parameters, biochemicalparameters, children

  3. Effects of dietary administration of yarrow extract on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss

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    Mahmoud Nafisi Bahabadi

    2014-10-01

    Full Text Available The present study was conducted to investigate the clinical effects and possible side effects of yarrow extract (Achillea millefolium L. as feed additive on biochemical blood parameters and growth performance of rainbow trout (Oncorhynchus mykiss. Fishes were treated with 0 (control, 0.1, 0.5 and 1% of yarrow extract for 30 days. Plasma alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, alkaline phosphatase (ALP, creatine kinase (CK, peroxidase activity, total complement and lysozyme activity, glucose, total protein, triglyceride and cholesterol were measured after 15 and 30 days of yarrow treatment. There were no significant changes in the lysozyme activity and glucose levels. Total protein and globulin levels were significantly higher in the fish fed with diets enriched with 1% yarrow extract on day 30. Triglyceride and cholesterol levels was significantly decreased in the fish fed with diets containing 0.5% and 1% yarrow extract on day 30 (P<0.05. LDH, CK and peroxidase activities in the fish fed with diets having 1% yarrow extract were significantly decreased at the end of the experiment (P<0.05. In contrast, a significant increase in AST, ALP and total complement activity was observed in the fish fed with 1% yarrow extract diet, on day 15 (P<0.05. The weight gain and specific growth rate increased and food conversion ratio decreased in in the fish fed 1% yarrow extract on day 30. Condition factor in the fish fed with yarrow extract was significantly higher than control group on 30 day. In conclusion, on the basis of these results, oral administration of yarrow extract up to 0.5% have not side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 1% of yarrow extract caused cytotoxicity and modifications in blood biochemical parameters of fish.

  4. Haematological and serum biochemical parameters of West African Dwarf goats fed dried cassava leaves-based concentrate diets.

    Science.gov (United States)

    Oni, Adebayo Olusoji; Arigbede, Oluwasanmi Moses; Sowande, Olusiji Sunday; Anele, Uchenna Young; Oni, Oluwakemi Oluremilekun; Onwuka, Chryss Friday Ijeoma; Onifade, Olufemi Sunday; Yusuf, Kafayat Omowumi; Dele, Peter Aniwe; Aderinboye, Ronke Yemisi

    2012-03-01

    The study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of West African Dwarf (WAD) goats. The study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. At the start of the experiment, packed cell volume (PCV) ranged from 21.5% to 25.5% while haemoglobin concentration (Hb) and RBC significantly (P  0.05). Lymphocyte reduced significantly (P  0.05) at the 0% to 40% levels and reduced at the 60% level of dried cassava leaves inclusion. At the start of the experiment, values for glucose significantly (P < 0.05) ranged from 40.1 to 56.0 mg/dl. Total protein and albumin values ranged significantly (P < 0.05) from 56.0 to 68.5 g/dl and 30.6 to 38.4 g/dl, respectively. At the end of the experiment, serum creatinine increased significantly (P < 0.05) as the level of dried cassava leaves increased from 0% to 60% in the diets. The study revealed that inclusion of dried cassava leaves in the diets of West African Dwarf goats had no deleterious effects on the haematological and serum biochemical parameters of WAD goats and could therefore be included in ruminant diets up to 60%. PMID:21744028

  5. THE VARIATION OF SOME BIOCHEMICAL PARAMETERS FROM FRESH COW CHEESE CREAM TYPE WITH ADDED AROMATIC PLANTS

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    CRISTIANA DIACONESCU

    2013-12-01

    Full Text Available Recent advanced studies in the field of cheese fabrication technology include, amongother, the usage of aromatic plants and spices as a mean of improving theorganoleptic properties and as a mean of decreasing the lipidic peroxidationphenomenon. For this purpose, three types of fresh cow cheese produced by S.C.Marion Invest Trade 94 SRL, experimentally added with dill, savory and rosemary,were comparatively studied from a chemical point of view (pH, acidity, humidity, ashand a biochemical point of view(raw fat, total proteins, total cholesterol, acidphosphatase’s enzymatic activity, superoxide dismutase’s enzymatic activity, calciumcontent.From a chemical point of view, no significant differences appeared between the creamcheese types analyzed, excepting the pH( lower in the cheeses with dill and rosemaryFrom a biochemical point of view, two phenomenon can be observed: a significantdecrease of the acidic phosphatase activity in the cheeses with added dill androsemary ( positively correlating with the pH value and decreased values of the SODactivity in all cheese types.

  6. Intestinal Alterations, Basal Hematology, and Biochemical Parameters in Adolescent Rats Fed Different Sources of Dietary Copper.

    Science.gov (United States)

    Tomaszewska, Ewa; Dobrowolski, Piotr; Kwiecień, Małgorzata

    2016-05-01

    Copper (Cu) is required for basically all biochemical and physiological processes in the body. The aim was to evaluate the effects of different sources of dietary copper on jejunal epithelium histomorphometry in adolescent rats. Male rats at the age of 5 weeks were used in the 12-week experiment. The control group was fed with standard diet providing the required Cu level (5 mg/kg body weight (bw) per day) in an inorganic form (sulfate) covered 100 % of daily demand, and the other three groups were supplemented with Cu-glycine complex covered 50, 75, and 100 % daily demand. Basal hematological and plasma biochemical analyses as well as histomorphometric examinations of the jejunal epithelium and liver were performed. Cu given in the organic form in 100 % of daily demand depressed the muscular and submucosa layer and the crypt depth (P < 0.05) without an influence of the innervation of the jejunum. In turn, organic Cu given in 75 % of daily demand did not influence the intestinal morphology in adult rats. Dietary organic Cu given to rats covering the daily demand in 50 or 75 % appears to be less harmful with regard to the intestinal epithelium than when administered in 100 % of daily demand. PMID:26432448

  7. Effect of imatinib on the biochemical parameters of the reproductive function in male Swiss albino mice

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    A M Prasad

    2011-01-01

    Full Text Available Background: Treatment of cancers with cytotoxic agents such as tyrosine kinase inhibiting drugs often, but not always, result in transient to permanent testicular dysfunction. Germ cells are important targets of many chemicals. Most of the drugs are genotoxins and induce irreversible effect on genetic makeup. These mutagenic changes are proportionally related to carcinogenesis. This is alarmingly dangerous in youth and children, since these effects last longer, affecting fertility or forming basis for carcinogenesis. There is paucity of reports on planned studies of imatinib on the testicular function. Hence, the study was planned to assess the effects of imatinib on biochemical markers of testicular functions in male Swiss albino mice. Materials and Methods: Male Swiss albino mice were treated with imatinib and sacrificed at the end of first, second, fourth, fifth, seventh, and tenth week after the last exposure to imatinib. The testis were removed, weighed, and processed for biochemical analysis. Results: The intratesticular testosterone level was significantly (P<0.001 reduced in treated groups and severe effect was observed on week 4 and 5. The intratesticular lactate dehydrogenase (LDH level was significantly increased by imatinib in all treated groups up to week 5. Conclusion: Imatinib does affect testosterone and LDH level significantly, but this effect is reversible once the drug is withdrawn. This finding may help the clinicians to plan and address the fertility-related issues in young patients of reproductive age who are being treated with imatinib for gastrointestinal tumors and chronic myeloid leukemia.

  8. INVESTIGATION OF EFFECT OF MURRAYA KOENIGII ON BIOPHYSICAL AND BIOCHEMICAL PARAMETERS OF WOUND IN DIABETIC HYPERLIPIDEMIC WISTAR RATS

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    Vikram Kumar*, Angshu Bandyopadhyay and Vikram Sharma

    2012-06-01

    Full Text Available Plants have been used in traditional medicine for several thousand years. India is perhaps the largest producer of medicinal herbs and is rightly called the “Botanical garden of the World”. Murraya koenigii Linn. commonly known as Meethi neem, belongs to the family Rutaceae. The curry tree is native to India and it is found almost everywhere in the Indian subcontinent excluding the higher levels of Himalayas. The effect of Murraya koenigii leaves aqueous extract on biophysical and biochemical parameters of wound were studied by dead space wound model in diabetic hyperlipidemic rats. In dead space wound model, animals treated with Murraya koenigii (oral administration of variable dosage level 200mg/kg, 300mg/kg and 400mg/kg leaves aqueous extract showed significant increase in Wet & Dry granulations tissue weight (biophysical parameter and hydroxyprolin content (biochemical parameter when compared to the diabetic hyperlipidemic control group rats. In this study, very significant (p<0.001 result was found with 300mg/kg dose level because the effect was dose dependent up to 300mg equivalent of extract. The results suggested that aqueous extract of Murraya koenigii possess significant wound healing potential in diabetic hyperlipidemic rats. Further studies may reveal the exact mechanisms of action responsible for the wound healing activity of Murraya koenigii leaves aqueous extract in diabetic hyperlipidemic condition.

  9. Influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals

    International Nuclear Information System (INIS)

    In the work authors inform results on studying influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals. It is established that all samples of a productive solution of uranium possesses high toxicity and causes destruction of experimental animals with infringement of a picture of blood. Experiments on influence on biochemical and hematology defined parameters of blood on chickens - broilers and on which female quails from daily age raised on cellular batteries in communities on 50 heads in conditions adequate to the requirement. Fed in plenty with the dry forages prepared according to 'Recommendations on the normalized feeding an agricultural bird' (1996), recipes 5-2; 6-1. To chickens in plenty allowed water from auto drinking bowls. Conditions of a micro climate corresponded to hygienic requirements. At carrying out of experiences studied the following parameters: preservation of a livestock, the reason of a withdrawal, weight of a body - weekly up to the end of experience, consumption of a forage, an expense of a forage for 1 kg of a gain have established weight of a body the methods standard in poultry farming. After application of a productive solution took blood from under wings of a vein and in blood have established maintenance of hemoglobin, quantity of red cells, leukocytes and a leukocyte of the formula, the maintenance of calcium and phosphorus. An application of a productive solution for experimental birds for the fifth day has caused clinical symptoms of a poisoning and destruction in groups. At the survived birds in blood it has been established changes biochemical and hematology. Week age chickens of skilled group lagged behind in growth development in comparison with control group on 30 % and at the end of the skilled period skilled have lagged behind in growth and development on 62 %. Among a livestock of chickens of skilled group of a case was more on 53 % on comparisons with the

  10. Effect of Pine Oil on Antihyperglycemic and Some Biochemical Parameters in serum of Streptozotocin Induced Tip-2 Diabetic rats

    OpenAIRE

    Ersin DEMİR; Yilmaz, Ökkeş

    2013-01-01

    The present study was designed to evaluate the effect of pine oil on hyperglycemia and some biochemical parameters in streptozotocin-induced type- 2 diabetic rats. Rats were divided into three groups: control (C) diabetes (STZ-DM), diabetes+pine oil (STZ-DM+PO) grups. Diabetes induced in rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). 1 ml/kg the dose pine oil was intraperitoneally injected twice in a week to the diabetes+pine oil (STZ-DM+PO), plus 1 g/L dose of pure ...

  11. Reference values for selected hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats

    OpenAIRE

    ŞİMŞEK, Özkan; ARIKAN, ŞEVKET; ÇINAR, Miyase

    2015-01-01

    The aim of this study was to monitor some hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats. In the present study, 35 blood samples obtained before and during pregnancy from 7 adult cats were used. Blood was collected from cats before pregnancy and on days 15, 30, 45, and 55 of gestation. The blood samples were analyzed in terms of WBC, RBC, Hb, PCV, MCV, MCH, MCHC, GRA, MON, and LYM values. Additionally, serum ALT, AST, ALP, GGT, LDH, and C...

  12. The dynamics of biochemical parameters in blood of clinically healthy holstein cows from day 5 before to day 60 after calving

    OpenAIRE

    Irena Celeska; Aleksandar Janevski; Igor Dzadzovski; Igor Ulchar; Danijela Kirovski

    2015-01-01

    The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and ...

  13. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

    Directory of Open Access Journals (Sweden)

    Ye. Barylo

    2016-06-01

    Full Text Available Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae of brown trout, rainbow trout and brook trout we used as study materials. Morphometric parameters we studied by the methods of N. O. Lange, E. N. Dmitrieva. The content of total lipids was determined in accordance with Folch. in the tissuesm, which were taken for biochemical studies. Separate classes of lipids were received by thin layer chromatography. Findings. We carried out a comparative analysis of morphometric measurements and biochemical parameters of one-day pre-larval brown trout, rainbow trout and brook trout based on the obtained data. We investigated morphometric and biochemical specific features of pre-larvae in post-embryonic period and showed the species differences of morphometric measurements. Significant differences were observed between the content of lipids in the body and yolk sac of free embryos. In particular, a higher content of phospholipids and triglycerides was observed in the body of brook trout compared to brown trout. We also recorded higher contents of mono- and diacylglycerols, free cholesterol, non-etherified fatty acids (NEFA, triacylglycerols and cholesterol esters in the yolk sac of brook trout. Compared to brown trout, rainbow trout had a significant increase in mono- and diacylglycerols, free cholesterol and NEFA in both body and yolk sac as well higher levels of total lipids, triacylglycerols and cholesterol esters were registered in yolk sac. Originality. For the first time we carried out and compared the specific features of pre-larval brown trout, rainbow trout and brook trout in the

  14. Improved Differential Evolution Algorithm for Parameter Estimation to Improve the Production of Biochemical Pathway

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    Chuii Khim Chong

    2012-06-01

    Full Text Available This paper introduces an improved Differential Evolution algorithm (IDE which aims at improving its performance in estimating the relevant parameters for metabolic pathway data to simulate glycolysis pathway for yeast. Metabolic pathway data are expected to be of significant help in the development of efficient tools in kinetic modeling and parameter estimation platforms. Many computation algorithms face obstacles due to the noisy data and difficulty of the system in estimating myriad of parameters, and require longer computational time to estimate the relevant parameters. The proposed algorithm (IDE in this paper is a hybrid of a Differential Evolution algorithm (DE and a Kalman Filter (KF. The outcome of IDE is proven to be superior than Genetic Algorithm (GA and DE. The results of IDE from experiments show estimated optimal kinetic parameters values, shorter computation time and increased accuracy for simulated results compared with other estimation algorithms

  15. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

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    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination. The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth condition of the respective colony. All the samples coming from sick colonieswere removed, only healthy adult honeybees coming from strong colonies were keptand used for hemolymph collection. By special methods, samples of hemolymph (300μl/sample collected from about 50 individuals, were analyzed both during the activeseason (spring/summer and the inactive season (autumn/winter. The study wascarried out on 50 samples of undiluted hemolymph taken from a total number of about2,500 honeybees. The following 21 biochemical parameters were analyzed: GLU(mg/dl, HDL-c (mg/dl, ALP (UI/l, T-cho (mg/dl, Tprot (mg/dl, Alb. (g/dl, BUN(mg/dl, LDH (UI/l, CPK (UI/l, Mg (mg/dl, FRA (μm/l, IP (mg/dl, GGT (UI/l,GOT (UI/l, GPT (UI/l, Ca (mg/dl, Cre (mg/dl, Amy (UI/l, T–BIL (mg/dl, TG(mg/dl, UA (mg/dl. The test was carried out after the collection and processing of thesamples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique,respectively.

  16. Impact of iron overload on interleukin-10 levels, biochemical parameters and oxidative stress in patients with sickle cell anemia

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    Maritza Cavalcante Barbosa

    2013-01-01

    Full Text Available OBJECTIVE: The aim of this study was to evaluate the impact of iron overload on the profile of interleukin-10 levels, biochemical parameters and oxidative stress in sickle cell anemia patients. METHODS: A cross-sectional study was performed of 30 patients with molecular diagnosis of sickle cell anemia. Patients were stratified into two groups, according to the presence of iron overload: Iron overload (n = 15 and Non-iron overload (n = 15. Biochemical analyses were performed utilizing the Wiener CM 200 automatic analyzer. The interleukin-10 level was measured by capture ELISA using the BD OptEIAT commercial kit. Oxidative stress parameters were determined by spectrophotometry. Statistical analysis was performed using GraphPad Prism software (version 5.0 and statistical significance was established for p-values < 0.05 in all analyses. RESULTS: Biochemical analysis revealed significant elevations in the levels of uric acid, triglycerides, very low-density lipoprotein (VLDL, alanine aminotransferase (ALT, lactate dehydrogenase (LDH, urea and creatinine in the Iron overload Group compared to the Non-iron overload Group and significant decreases in the high-density lipoprotein (HDL and low-density lipoprotein (LDL. Ferritin levels correlated positively with uric acid concentrations (p-value < 0.05. The Iron overload Group showed lower interleukin-10 levels and catalase activity and higher nitrite and malondialdehyde levels compared with the Non-iron overload Group. CONCLUSION: The results of this study are important to develop further consistent studies that evaluate the effect of iron overload on the inflammatory profile and oxidative stress of patients with sickle cell anemia.

  17. A comparative study of the hemato-biochemical parameters between clinically healthy cows and cows with displacement of the abomasum

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    Zadnik T.

    2003-01-01

    Full Text Available The objective of this study was to evaluate hemato-biochemical parameters in cows with left- and right- sided displacement of the abomasum. Comparison of a group of cows with displacement of the abomasum (n = 73 and a control group of cows (n=43 of the same breed, age, physiological period and management showed statistically significant (p<0.05 deviations of the following hematological and biochemical parameters: increased hematocrit (Ht, white blood cell count (WBCC, neutrophils, total bilirubin (Bil-T glucose, b-hydroxybutyrate, non-esterified fatty acids (NEFA, serum enzymatic activity of aspartate aminotransferase (AST, g-glutamyl transferase (GGT and glutamate dehydrogenase (GLDH, together with decreased calcium (Ca, sodium (Na, potassium (K and chloride (Cl concentration. Blood analyses in cows with displacement of the abomasum clearly indicated association of the disease with postpartum disease syndromes such as hypocalcaemia, hepatic lipidosis and endotoxemia. Thus hemoconcentration, leukocytosis with neutrophilia, hypocalcaemia, lipemia ketonemia, hyperglycemia, slight hypernatremia, hypokalemia, hypochloremia and increased activity of liver enzymes (AST, GGT, GLDH in blood serum were observed in these cows in comparison to the control group. All established irregularities cause complicated pathophysiological metabolic reactions which are often clinically manifested as displacement of the abomasum during the periparturient period.

  18. The Effects of Air Pollution and Smoking on Cadmium Concentration in Human Blood and Correlation with Biochemical Parameters

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    L. Zeneli

    2009-01-01

    Full Text Available Problem statement: The study described the research of the effects that the environment pollution and smoking have in cadmium concentration in human blood, as well as in the correlation between cadmium and the biochemical parameters. Approach: In a comparative study of cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants (Obiliq, a highly polluted environments (Investigated Group and the other that was considered as relatively clean rural environment Dragash (control group. Results: The results showed that there exists a significant difference in the average concentration of cadmium in human blood between the Investigated Group (IG and the Control Group (CG (t = -3.34, p = 0.0006. The series of determination of cadmium concentration in blood of population that lives in this environment had shown direct effects in biochemical parameters (direct bilirubine, total bilirubine. Conclusion: Air pollution (from coal burning in power plant and smoking were very important factors for the level of cadmium concentration in blood, which had an inhibitory effect in the syntheses of bilirubine.

  19. Determination of the regulatory properties of Yucca schidigera extracts on the biochemical parameters and plasma hormone levels associated with obesity

    Directory of Open Access Journals (Sweden)

    Ismail Kucukkurt

    2016-04-01

    Full Text Available ABSTRACT Yucca schidigera Ortgies, Asparagaceae, is a herbaceous plant. Due to the high saponin content the powdered branches and leaves are used as natural food additive for human and animal. The aim of this study was to investigate the effects of Y. schidigera extracts on plasma leptin, ghrelin, adiponectin, insulin, thyroid hormones and some biochemical parameters in mice fed a high-fat diet. Male Swiss Albino mice were divided into seven equal groups. Group I (negative control group was given standard diet; Group II was given high-fat diet; Group III was given high-fat diet with carboxymethylcellulose; Groups IV–VII were given hexane, petroleum ether, ethyl acetate, and methanol extracts of Y. schidigera and high-fat diet via gastric gavage for 60 days. High-fat diet significantly increased plasma leptin, insulin, free T3 hormone, glucose, cholesterol, low-density lipoprotein, triacylglyceride, aspartate aminotransferase and alanine aminotransferase levels, and significantly decreased plasma ghrelin, adiponectin and free T4 hormone levels. On the other hand, hormone levels, lipid profile and biochemical parameters were improved by the administration of the PE extract. Y. schidigera extracts could be used as preventive medicine in nutritional disorders via regulating energy metabolism and hormonal functions.

  20. Effects of Pre-induction Administration of Allium Sativum on Some Biochemical Parameters in Alloxan Induced Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Ojo Rotimi Johnson

    2012-12-01

    Full Text Available The aim of the present study was to investigate the effects of pre- and post- administration of garlic extract on serum glucose and some biochemical parameters in alloxan-induced diabetic rats to show the preventive and ameliorating effects in alloxan induced-diabetic rats. Rats were divided into 4 groups; normal control rats, diabetic control rats, diabetic rats post-treated with garlic extract and rats pre-treated with garlic extract before induction. Garlic extract was administered orally for 2 weeks to post-treated rats and 3 weeks to pre-treated rats and they were compared with the normal and diabetic groups, respectively. Serum glucose was reduced significantly in both post-treated and pre-treated groups. The post-treatment with garlic extract reduced serum cholesterol, but pre-treatment with garlic extract produced significant change compare to the diabetic control. The serum creatinine and urea levels were significantly reduced in post-treated group and pretreated group compare to the diabetic control group. It is concluded that the consumption of garlic produced a significant hypoglycaemic and hypolipidemic effects in diabetic rats and also, garlic is capable of protecting the liver and the kidney functions in alloxan-induced diabetic rats as shown in the activities of serum enzymes and other biochemical parameters examined.

  1. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

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    Mirjana Joksimović Todorović

    2012-06-01

    Full Text Available The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA, activity of enzymes of aspartat aminotransferase (AST, alanin aminotransferase (ALT and gamma glutamyltranferase (GGT, in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartum. The highest values of total leukocytes count, neutrophiles, monocytes and basophiles count were recorded on the day of parturition, and then decreased, so that the minimum value established on d 45 of lactation. The lymphocytes count was the lowest on the day of parturation, while the eosinophils and basophiles counts were significantly different in observed time intervals. The concentration of glucose ranged from 3.07 mmol/L on d 15 prepartum to 2.71 mmol/L on d 15 postpartum. A high concentration of non-esterified fatty acids (NEFA in the postpartum period is a consequence of changes in energy balance and more intensive process of lypolisis in fat tissue, due to energy disbalance at the beginning of lactation. Established activity of enzymes AST, ALT and GGT was approximately the same in studied time intervals and was within physiological limits. The results of hematological and biochemical analysis in this paper do not indicate the development of postpartum ailments, because the investigated parameters were within physiological limits.

  2. STUDY OF BIOCHEMICAL PARAMETERS IN AMNIOTIC FLUID FOR ASSESSMENT OF FOETAL MATURITY IN CASES OF NORMAL PREGNANCY

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    Leela

    2015-11-01

    Full Text Available Assessment of foetal maturity had been proven of value in evaluating the foetal condition. Accurate assessment of foetal maturity is essential for the proper timing of delivery in various risk pregnancies. Amniotic Fluid analysis for foetal maturity had been of proven value. In the present study, study of biochemical parameters in amniotic fluid in respect of Creatinine, Uric Acid, Urea, Total Proteins, and Electrolytes i.e. Sodium, Potassium and Chloride has been done, along with Serum Electrolytes. Standard methodologies were adopted. The observations in the present study correlated with the works of Chadick et al and Pitkin and Zwirek. The levels of Creatinine, Uric Acid and Urea in Amniotic Fluid showed elevation, while Total Proteins and Serum Sodium showed a decline, as gestation progressed. The Serum and Amniotic Fluid Potassium and Chloride levels remain almost constant throughout the pregnancy. Thus, it is observed that the use of multiple parameters is desirable for accurate assessment of foetal maturity.

  3. [Biochemical parameters of blood plasma of the harp seal Pagophilus groenlandicus Erxleben, 1777 (Pinnipedia, Procidae) of various ages].

    Science.gov (United States)

    Erokhina, I A

    2007-01-01

    Results of study of several blood biochemical parameters of the harp seal Pagophilus groenlandicus Erxleben, 1777, from birth to the adult state are presented. The following age groups of the animals have been studied--newborns, 1.5-2 months, 1 year, 2 years, 3 years, and adults (older than 6 months). The content of total protein and its fractions, free amine nitrogen, urea, creatinine, glucose, total lipids, total calcium, and inorganic phosphorus was determined in the seal blood plasma. Differences of the degree of expression of levels of all studied parameters in the course of early postnatal ontogenesis of the animals have been established. The most significant metabolic changes in the harp seals were revealed at the period of end of weaning and transition to independent ingestion and then after the first year of life. PMID:17725029

  4. THE EFFECT OF THE KETOGENIC DIET ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF THE CHILDREN WITH RESISTANT EPILEPSY

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    Mohammadreza ALAEI

    2010-03-01

    Full Text Available ObjectiveThe aim of this study was to evaluate the effect of the ketogenic diet on the growth parameters of the children with resistant epilepsy.Materials & MethodsA total of 36 children with resistant epilepsy who were 2 to 7 year old were put on the ketogenic diet. Their growth and biochemical parameters were studied at the beginning of the study and after 3 months.ResultsWeight decreased in all patients. Serum levels of hemoglobin, calcium, and blood sugar decreased significantly but remained in the normal range. Creatinine did not change, but BUN showed a significant increase.ConclusionWe can lower the complications of ketogenic diets by using more unsaturated fat, more water, and more minerals.

  5. Long-term changes in microbial and biochemical parameters in the Central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Raghukumar, C.; Nath, B.N.; Sharma, R.; LokaBharathi, P.A.; Dalal, S.G.

    an artificial sediment disturbance was created by a hydraulic benthic disturber. Besides the above- mentioned parameters, extracellular enzymes, alkaline phosphatase, aminopeptidase and lipase were also monitored at these 9 locations. Postdisturbance (PoD1...

  6. Modeling photosynthesis of discontinuous plant canopies by linking the Geometric Optical Radiative Transfer model with biochemical processes

    Science.gov (United States)

    Xin, Q.; Gong, P.; Li, W.

    2015-06-01

    Modeling vegetation photosynthesis is essential for understanding carbon exchanges between terrestrial ecosystems and the atmosphere. The radiative transfer process within plant canopies is one of the key drivers that regulate canopy photosynthesis. Most vegetation cover consists of discrete plant crowns, of which the physical observation departs from the underlying assumption of a homogenous and uniform medium in classic radiative transfer theory. Here we advance the Geometric Optical Radiative Transfer (GORT) model to simulate photosynthesis activities for discontinuous plant canopies. We separate radiation absorption into two components that are absorbed by sunlit and shaded leaves, and derive analytical solutions by integrating over the canopy layer. To model leaf-level and canopy-level photosynthesis, leaf light absorption is then linked to the biochemical process of gas diffusion through leaf stomata. The canopy gap probability derived from GORT differs from classic radiative transfer theory, especially when the leaf area index is high, due to leaf clumping effects. Tree characteristics such as tree density, crown shape, and canopy length affect leaf clumping and regulate radiation interception. Modeled gross primary production (GPP) for two deciduous forest stands could explain more than 80% of the variance of flux tower measurements at both near hourly and daily timescales. We demonstrate that ambient CO2 concentrations influence daytime vegetation photosynthesis, which needs to be considered in biogeochemical models. The proposed model is complementary to classic radiative transfer theory and shows promise in modeling the radiative transfer process and photosynthetic activities over discontinuous forest canopies.

  7. Effect of gamma irradiation on seed germination, growth and biochemical parameters of Terminalia arjuna ROXB

    International Nuclear Information System (INIS)

    Terminalia arjuna, a potent medicinal plant for the treatment of cardiac disease was chosen for the study. About 300 seeds of T. arjuna were irradiated with different doses (0, 10, 25, 50, 100, 150 and 200 Gy) of gamma radiation using 60Co gamma source. Effect of gamma radiation on growth and biochemical constituents were compared with the control of plants. Germination speed at 25 Gy was found to be 0.65 which was double the control plants. An increase in germination percentage, vigour index and relative growth rate in terms of dry weight was noticed at lower dosages. There was a gradual increase in shoot and root length at 25 and 50 Gy which decreased at higher doses of radiation. Proline content increased with increasing doses of radiation. Chlorophyll content was found to be 12.2 mg/gfw at 100 Gy while in control it was 8.44 mg/gfw. Increased phenolic content and radical scavenging capacity was observed at 25 Gy and 150 Gy. Hence, lower doses of radiation treatment to seeds may be recommended to increase the germination, growth and to increase secondary plant products. (author)

  8. Effect of gamma irradiation on seed germination, growth and biochemical parameters of Flacourtia Montana Graham

    International Nuclear Information System (INIS)

    Flacourtia montana an endemic species of Western Ghat which is well known for its ethno-medicinal value was chosen for the study. Matured seeds collected from Beithangady region of Dakshina Kannada district were irradiated with different doses (0, 25, 50, 100, 150, 200, 250, 300 Gy) of gamma radiation using 60Co gamma source at Board of Radiation and Isotope Technology (BRIT). Effect of gamma radiation on growth and biochemical constituents were compared with that of control plants. An increase in germination percentage, speed and vigour index was observed at lower dosages, and the dose of 100 Gy was found to be more effective out of the selected doses as far as growth is concerned. Doses of 25 and 100 Gy showed 95 and 100% germination respectively but the germination was reduced at higher dosages. Total chlorophyll content at 10 Gy was found to be 19.56 mg/gfw while in control was 14 mg/gfw. Phenolic content was higher at 50 Gy and proline content at 150 Gy. Slight increase in carbohydrate content was observed in irradiated samples. Hence, lower doses of radiation treatment may be recommended to increase growth and phenolic content of plant F.montana. (author)

  9. Correlation between ovarian morphology and biochemical and hormonal parameters in polycystic ovary syndrome

    Science.gov (United States)

    Inan, Cihan; Karadag, Cihan

    2016-01-01

    Objective: To determine the biochemical and hormonal differences in polycystic ovary syndrome (PCOS) patients with and without polycystic ovary (PCO) morphology and to evaluate the outcomes resulting from those differences. Methods: The study included a total of 83 patients with PCOS; 43 of them had PCO morphology (Group-I) and 40 did not (Group-II). Serum LDL, HDL, total cholesterol, triglyceride (TG), total testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), 17b-estradiol (E2), prolactin (PRL), thyroid stimulating hormone (TSH), sex hormone binding globulin (SHBG), glucose and insulin levels were determined. Homoeostatic model assessment insulin resistance (HOMA-IR) index was calculated. Results: The two groups were similar with respect to BMI. The systolic and diastolic blood pressure measurements of Group-I were significantly lower (p<0.01). Serum mean level of LH (p=0.026) and the mean LH/FSH (p=0.001) level of Group-I were significantly higher than Group-II. The total cholesterol and triglyceride levels of Group-I were significantly lower (p<0.05, p<0.01). The mean HOMA-IR level of Group-I was significantly lower than Group-II (p=0.004). Conclusions: The group without PCO morphology had a higher risk than the other group in terms of increased insulin resistance, dyslipidemia and cardiovascular diseases due to effects of hyperandrogenism. PMID:27375725

  10. Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L.

    Science.gov (United States)

    Bagheri, Mahsa; Mansouri, Hakimeh

    2015-03-01

    This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. PMID:25492688

  11. Association of renal biochemical parameters with left ventricular diastolic dysfunction in a community-based elderly population in China: a cross-sectional study.

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    Jingmin Zhou

    Full Text Available BACKGROUND: Relationship of left ventricular diastolic dysfunction (LVDD with parameters that could provide more information than hemodynamic renal indexes has not been clarified. We aimed to explore the association of comprehensive renal parameters with LVDD in a community-based elderly population. METHODS: 1,166 community residents (aged ≥ 65 years, 694 females participating in the Shanghai Heart Health Study with complete data of renal parameters were investigated. Echocardiography was used to evaluate diastolic function with conventional and tissue Doppler imaging techniques. Serum urea, creatinine, urea-to-creatinine ratio, estimated glomerular filtration rate (eGFR and urinary albumin-to-creatinine ratio (UACR were analyzed on their associations with LVDD. RESULTS: The prevalence of LVDD increased in proportion to increasing serum urea, urea-to-creatinine ratio and UACR. These three renal parameters were found negatively correlated to peak early (E to late (A diastolic velocities ratio (E/A, and positively to left atrial volume index; UACR also positively correlated with E to peak early (E' diastolic mitral annular velocity ratio (E/E'. Serum urea, urea-to-creatinine ratio and UACR correlated with LVDD in logistic univariate regression analysis, and urea-to-creatinine ratio remained independently correlated to LVDD [Odds ratio (OR 2.82, 95% confidence interval (CI 1.34-5.95] after adjustment. Serum urea (OR 1.18, 95%CI 1.03-1.34, creatinine (OR 6.53, 95%CI 1.70- -25.02, eGFR (OR 0.22, 95%CI 0.07-0.65 and UACR (OR 2.15, 95%CI 1.42-3.24 were revealed independent correlates of advanced (moderate and severe LVDD. CONCLUSIONS: Biochemical parameters of renal function were closely linked with LVDD. This finding described new cardio-renal relationship in the elderly population.

  12. Effect of maternal exercise on biochemical parameters in rats submitted to neonatal hypoxia-ischemia.

    Science.gov (United States)

    Marcelino, Thiago Beltram; de Lemos Rodrigues, Patrícia Idalina; Miguel, Patrícia Maidana; Netto, Carlos Alexandre; Pereira Silva, Lenir Orlandi; Matté, Cristiane

    2015-10-01

    Pregnancy is a critical period for brain metabolic programming, being affected by individual environment, such as nutrition, stress, and physical exercise. In this context, we previously reported a cerebral antioxidant upregulation and mitochondrial biogenesis in the offspring delivered from exercised mothers, which could provide neuroprotection against neonatal insults. Hypoxia-ischemia (HI) encephalopathy is one of the most studied models of neonatal brain injury; disrupting motor, cognitive, and learning abilities. Physiopathology includes oxidative stress, allied to mitochondria energy production failure, glutamatergic excitotoxicity, and cell death. In this study we evaluated the effect of maternal swimming during pregnancy on offspring׳s brain oxidative status evaluated fourteen days after HI stablishment. Swimming exercise was performed by female adult rats one week before and during pregnancy, in controlled environment. Their offspring was submitted to HI on postnatal day 7, and the brain samples for biochemical assays were obtained in the weaning. Contrary to our expectations, maternal exercise did not prevent the oxidative alterations observed in brain from HI-rats. In a general way, we found a positive modulation in the activities of antioxidant enzymes, measured two weeks after HI, in hippocampus, striatum, and cerebellum of pups delivered from exercised mothers. Reactive species levels were modulated differently in each structure evaluated. Considering the scenery presented, we concluded that HI elicited a neurometabolic adaptation in both brain hemispheres, particularly in hippocampus, parietal cortex, and cerebellum; while striatum appears to be most damaged. The protocol of aerobic maternal exercise was not enough to fully prevent HI-induced brain damages. PMID:26119914

  13. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    Energy Technology Data Exchange (ETDEWEB)

    Raposo, F.; Borja, R.; Rincon, B. [Instituto de la Grasa (CSIC), Avda. Padre Garcia Tejero 4, 41012 Seville (Spain); Jimenez, A.M. [Departamento de Ciencias Ambientales, Universidad Pablo de Olavide, Ctra. De Utrera, km 1, 41013 Sevilla (Spain)

    2008-12-15

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (Y{sub M,ult}) decreased considerably from 227{+-}23 to 107{+-}11 ml CH{sub 4} at standard temperature and pressure (STP) conditions g{sup -1} VS{sub added} when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g{sup -1} VS{sub added} was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates. (author)

  14. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    International Nuclear Information System (INIS)

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 deg. C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (YM,ult) decreased considerably from 227±23 to 107±11 ml CH4 at standard temperature and pressure (STP) conditions g-1 VSadded when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g-1 VSadded was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates

  15. Stochastic analyses of field-scale pesticide leaching risk as influenced by spatial variability in physical and biochemical parameters

    Science.gov (United States)

    Loll, Per; Moldrup, Per

    2000-04-01

    Field-scale pesticide leaching risk assessments were performed by incorporating a numerical, one-dimensional, water and pesticide transport and fate model into the two-step stochastic modeling approach by Loll and Moldrup [1998]. The numerical model included first-order pesticide degradation, linear equilibrium adsorption, and plant uptake of water and pesticide. Simazine was used as a model pesticide, and leaching risk was expressed as the cumulative mass fraction of applied pesticide leached below 100 cm after 1 year. Spatial variability in soil physical and biochemical data, as well as measured meteorological data from an average and a relatively wet year, was considered for two Danish field sites: (1) a coarse sandy soil, with relatively small variability in hydraulic properties, and (2) a sandy loam, with large variability in hydraulic properties. The two-step stochastic modeling approach was used to investigate the relative impact of spatial variability in saturated hydraulic conductivity Ks, soil-water retention through the Campbell [974] soil-water retention parameter b, and pesticide sorption through the organic carbon content (OC). For the coarse sandy soil, field-scale spatial variability in OC was the single most important parameter influencing leaching risk, whereas for the sandy loam, Ks was found more important than OC. The relative impact of field-scale spatial variability in these parameters was found independent of the meteorological conditions, whereas the absolute level of leaching risk was highly dependent on the meteorological conditions. Assuming a linear dependency between pesticide half-life and OC, a unified approach to modeling simultaneous field-scale variability in biodegradation and adsorption was proposed. Leaching risk assessments based on this approach showed that the parts of the field with both low biological activity and low adsorption capacity contributed with a dramatic increase in leaching risk, and suggested that field

  16. Pharmacodynamic interaction of fenugreek, insulin and glimepiride on sero-biochemical parameters in diabetic Sprague-Dawley rats

    Directory of Open Access Journals (Sweden)

    C. Haritha

    2015-05-01

    Full Text Available Aim: This study was undertaken to assess the pharmacodynamic interaction of fenugreek, insulin and glimepiride on serobiochemical parameters in streptozotocin-induced diabetic rats. Materials and Methods: A total of 56 male Sprague-Dawley rats, randomly divided into seven Groups. Group 1: Non-diabetic control; Group 2: Streptozotocin induced diabetic control; Groups 3, 4 and 5 were treated with insulin, glimepiride and fenugreek seed powder, respectively; Groups 6 and 7: Insulin + fenugreek seed powder treatment and glimepiride + fenugreek seed powder treatment respectively, in diabetic rats. Body weights, blood glucose, lipids total cholesterol (TC, high-density lipoprotein (HDL, low-density lipoprotein (LDL, triglycerides (TG and proteins (total protein, albumin, globulin, A/G ratios were studied at different time intervals. Rats were sacrified at the end of 8 weeks, pancreas and aorta collected for histopathological study. Results: The results of Group 2 showed significantly (p<0.05 higher concentration of glucose, TC, TG, LDL, globulin, A/G ratios and significantly (p<0.05 lower concentration of albumin, total protein, HDL and body weights when compared to Group 1 at the end of 4th and 8th weeks intervals with marked alteration in histopathology of pancreas and aorta. All the treatment Groups 3-7 showed significantly (p<0.05 improvement in the all the parameters and the Groups 6 and 7 showed highest decrease in the concentration blood glucose, TC, TG, LDL and increase in the albumin, total protein and body weights during 6th and 8th week, respectively. Conclusion: The treatment with fenugreek, insulin and glimepiride countered the alteration in the sero biochemical parameters in diabetic rats, and their combination was found a positive interaction in improving the sero biochemical status of diabetic rats.

  17. RELATIONSHIP BETWEEN BIOCHEMICAL PARAMETERS - LIVER ENZYMES (AST, ALT AND ALP AND BLOOD PHENYTOIN LEVELS IN PATIENTS OF EPILEPSY

    Directory of Open Access Journals (Sweden)

    Ashok

    2013-11-01

    21 by the method of Dill. 5ml of blood was collected to measure the following biochemical parameters: Serum AST, Serum ALT, Serum ALP, Instrument – Ciba Corning Blood gas 288 system and other biochemical parameters are measured by Technicon - RA - XT Auto Analyser. CONCLUSION : The present study summarizes its findings in patients of grand - mal epilepsy receiving phenytoin therapy. Elevation of serum alkaline phosphatase levels in comparison to the control group. Other parameters such as serum aspartate transaminase and alanine transaminase were within normal limits and there was no significant correlation

  18. FPGA-Based Fused Smart Sensor for Dynamic and Vibration Parameter Extraction in Industrial Robot Links

    OpenAIRE

    Rene de Jesus Romero-Troncoso; Gilberto Herrera-Ruiz; Roque Alfredo Osornio-Rios; Luis Morales-Velazquez; Carlos Rodriguez-Donate

    2010-01-01

    Intelligent robotics demands the integration of smart sensors that allow the controller to efficiently measure physical quantities. Industrial manipulator robots require a constant monitoring of several parameters such as motion dynamics, inclination, and vibration. This work presents a novel smart sensor to estimate motion dynamics, inclination, and vibration parameters on industrial manipulator robot links based on two primary sensors: an encoder and a triaxial accelerometer. The proposed s...

  19. Evaluation of Nutritional Status and Its Effect on Biochemical Parameters in Elderly People Admitted to Home Health Care Services

    Directory of Open Access Journals (Sweden)

    Aydan Çevik1

    2014-09-01

    Full Text Available Objective: Malnutrition and under nutrition are common phenomena in elderly. Prevalence of malnutrition is even higher in older people admitted to home health care services. Our aim was to evaluate the nutritional status and its effect on biochemical parameters of the elderly people admitted to home health care services. Methods: Our study was performed in 178 participants admitted to the home health care services. Validated Mini Nutritional Assessment (MNA form was used and biochemical tests were performed. Results: Main age of the participants is 81.19±8.22 years and 32% of participants were male, 68% were women. According to MNA 33.1% were malnourished, 39.3% were under malnutrition risk and 27.5% were in normal nutritional status. Hemoglobin, total iron, total iron binding capacity (TIBC, vitamin B12, folic acid and lymphocyte levels were significantly lower, ferritin and C-reactive protein levels were significantly higher in malnutrition group. Total iron and albumin levels were significantly lower and CRP levels were significantly higher in under nutrition group compared to subjects with normal nutritional status. Conclusion: Elderly people given home care are under greater risk of malnutrition. If not appropriately managed, malnutrition is associated with higher morbidity and mortality. Therefore it is essential to closely monitor the nutritional situational status of the elderly people who are admitted to home health care services.

  20. Effect of Different Growth Conditions on Certain Biochemical Parameters of Different Cyanobacterial Strains

    Directory of Open Access Journals (Sweden)

    Hammouda, O. E.

    2012-01-01

    Full Text Available Aims: Variation in the traditional growth medium conditions to enhance the production of lipids, carbohydrates, protein and the free amino acids content of three cyanobacterial species. Methodology and Results: Three species of cyanobacteria (Anabaena laxa, Anabaena fertilissima and Nostoc muscorum were collected from the culture collection of Soils, Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt, to investigate their biochemical composition under different growth conditions, using BG110 (nitrogen free as growth medium. These conditions were represented by control medium, static glucose medium with (1%, w/v, aerated medium (aerated by bubbling technique depending on CO2 normally existed in air with a concentration of 0.03%, molasses medium (0.7%, v/v and aerated medium enriched with glucose (1%, w/v. Lipid content, total carbohydrates, soluble proteins and free amino acids were determined at the previous conditions. Glucose at 0.7% (w/v was the most favorable for lipid production in A. laxa, where it exhibited the highest lipid content (427 μg/g fresh wt.. Increasing molasses concentration up to 0.7% (v/v produced an increase in lipid contents of the tested cyanobacterial strains. The highest lipid content of both N. muscorum (366.2 μg/g fresh wt. and A. laxa (357.4 μg/g fresh wt. were recorded at molasses concentrations of 0.1 and 0.7% (v/v, respectively. A. laxa expressed high significant values for both proteins (31.6 μg/mL and free amino acids (40.5 mg/g dry wt. after 6 days of incubation period under aerated enriched glucose condition (1%, w/v. Also, at the same growth conditions, A. fertilissima exhibited high significant values for carbohydrates at 4th day (876.8 mg/g dry wt.. Conclusion, significance and impact of study: Aerated enriched glucose medium (1%, w/v was the best growth medium condition used in the present study.

  1. Effect of wood ash application on the morphological, physiological and biochemical parameters of Brassica napus L.

    Science.gov (United States)

    Nabeela, Farhat; Murad, Waheed; Khan, Imran; Mian, Ishaq Ahmad; Rehman, Hazir; Adnan, Muhammad; Azizullah, Azizullah

    2015-10-01

    The present study was conducted to determine the effect of wood ash application on different parameters of Brassica napus L. including seed germination, seedling growth, fresh and dry biomass, water content in seedlings, photosynthetic pigments, soluble sugars, total protein and cell viability. In addition, the effect of wood ash on soil microflora and accumulation of trace elements in seedlings were determined. The seeds of B. napus were grown at different doses of wood ash (0, 1, 10, 25, 50 and 100 g (wood ash)/kg (soil)) and the effect on various parameters was determined. Wood ash significantly inhibited seed germination at doses above 25 g/kg and there was no germination at 100 g/kg of wood ash. At lower concentrations of wood ash, most of the growth parameters of seedlings were stimulated, but at higher concentrations of wood ash most of the studied parameters were adversely affected. Wood ash was found to be very detrimental to B. napus when applied above 25 g/kg. Wood ash application resulted in an increased bioaccumulation of trace elements in seedlings of B. napus. Almost all trace elements were significantly higher in seedlings grown in wood ash above 10 g/kg as compared to the control. An increase in total microbial count was observed with wood ash treatment which was statistically significant at 1 and 10 g/kg of wood ash. It is concluded that at very high concentration, wood ash can be detrimental to plants; however, its application at lower application rate can be recommended. PMID:26163419

  2. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

    OpenAIRE

    Danila Di Majo; Gabriella Schiera; Valentina Contrò; Elena Joana Armeli; Marcello Giaccone; Marco Giammanco; Marcello Traina; Antonio Palma; Patrizia Proia

    2014-01-01

    In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old) middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT)] and skeletal and myocardial markers of musc...

  3. Performance emulation and parameter estimation for nonlinear fibre-optic links

    DEFF Research Database (Denmark)

    Piels, Molly; Porto da Silva, Edson; Zibar, Darko;

    2016-01-01

    Fibre-optic communication systems, especially when operating in the nonlinear regime, generally do not perform exactly as theory would predict. A number of methods for data-based evaluation of nonlinear fibre-optic link parameters, both for accurate performance emulation and optimization, are...

  4. Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro

    Directory of Open Access Journals (Sweden)

    Sharuti Rathore

    2014-05-01

    Full Text Available Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in productivity. It is essential to test important medicinal plants for their salinity tolerance as research efforts aim to explore economic benefits under saline conditions. Keeping in view the importance of Stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two Stevia genotypes. Two node microcuttings were subjected to MS media supplemented with different NaCl concentrations (0, 25, 50, 75, 100, 125 mM. Chlorophyll amount was observed to be decreased as compared to sugars, proline and phenols with increased salt concentrations.

  5. Effect of dietary cation-anion balance on blood biochemical parameters and items in urine of dairy cows

    International Nuclear Information System (INIS)

    Twenty cows in mid-lactation were randomly ground into 4 diets with dietary cation-anion balance (DCAB, mEq/100 g dry matter) values of 87 (control), 300, 400 and 500 to measure the effect of DCAB on blood biochemical parameters and urine index. In blood, luteotropin significantly increased at DCAB 400 fed sample, estradiol (E2) and urea decreased at DCAB 400 and 500 fed sample. However, Lactate dehydrogenate, creatine phosphokinase and total protein had a significant increase for DCAB 300 and 400 fed sample. Calcium for all treatments significantly increased. In urine, Urea and pH significantly increased, but K+ reduced at DCAB 400 and 500 fed sample. Cl- was lower for all treatments, and Na+ was higher for DCAB 300 and 500 fed sample. Urea was enhanced at DCAB 400 feed. DCAB 400 is suited as the feed for midlactation cows. (authors)

  6. Toxic Effects of Lead on Growth and Some Biochemical and Ionic Parameters of Sunflower (Helianthus annuus L. Seedlings

    Directory of Open Access Journals (Sweden)

    Hamid Noorani Azad

    2011-07-01

    Full Text Available Lead (Pb is one of the non essential and toxic heavy metals which can cause oxidative stress in plants. The effects of Pb(NO32 toxicity on growth and some biochemical parameters of record cultivars of Helianthus annuus L. were studied under hydroponic condition. Different treatments of Pb(NO32 [control (0, 200, 400, 600 and 800 :M] were used in order to consider changes in dry weight, proline and Pb accumulation in roots and shoots; Total chlorophyll, enzyme activity (catalase and peroxidase and K+, Ca2+ amounts of leaves. Compared with the control, Pb treatment caused a significant decrease in roots and shoots dry weight, leaves chlorophyll, catalase activity and K+, Ca2+ amounts. In contrast, a significant increase in proline and Pb accumulation of roots and shoots and peroxidase activity of leaves was observed in Pb treatments.

  7. Three linked nomograms for predicting biochemical failure in prostate cancer treated with radiotherapy plus androgen deprivation therapy

    International Nuclear Information System (INIS)

    Nomograms were established to predict biochemical recurrence (BCR) after radiotherapy (RT) with a low weight of the characteristic variables of RT and androgen deprivation therapy (ADT). Our aim is to provide a new stratified tool for predicting BCR at 4 and 7 years in patients treated using RT with radical intent. A retrospective, nonrandomized analysis was performed on 5044 prostate cancer (PCa) patients with median age 70 years, who received RT - with or without ADT - between November 1992 and May 2007. Median follow-up was 5.5 years. BCR was defined as a rise in serum prostate-specific antigen (PSA) of 2 ng/ml over the post-treatment PSA nadir. Univariate association between predictor variables and BCR was assessed by the log-rank test, and three linked nomograms were created for multivariate prognosis of BCR-free survival. Each nomogram corresponds to a category of the Gleason score - either 6,7, or 8-10 - and all of them were created from a single proportional hazards regression model stratified also by months of ADT (0, 1-6, 7-12, 13-24, 25-36, 36-60). The performance of this model was analyzed by calibration, discrimination, and clinical utility. Initial PSA, clinical stage, and RT dose were significant variables (p < 0.01). The model showed a good calibration. The concordance probability was 0.779, improving those obtained with other nomograms (0.587, 0.571, 0.554) in the database. Survival curves showed best clinical utility in a comparison with National Comprehensive Cancer Network (NCCN) risk groups. For each Gleason score category, the nomogram provides information on the benefit of adding ADT to a specific RT dose. (orig.)

  8. Use of simple hematological, biochemical and clinical parameters to monitor response of multiple myeloma patients on high dose thalidomide therapy

    Directory of Open Access Journals (Sweden)

    Uppal G

    2005-01-01

    Full Text Available BACKGROUND: Evidence of increased bone marrow vascularity in multiple myeloma (MM has led to the use of anti-angiogenic drugs especially thalidomide in relapsed or refractory patients. Currently, parameters such as serum/ urine electrophoresis for M (monoclonal proteins, bone marrow biopsy with touch preparation and b2 microglobulin are routinely used to assess response to therapy. These investigations are expensive, invasive and require high technical setup. AIM: To correlate simple and routine hematological and biochemical parameters with the key marker of disease i.e. M proteins. SETTINGS AND DESIGN: This is an open label, uncontrolled, single-arm study. MATERIALS AND METHODS: Twenty nine refractory or relapsed multiple myeloma patients of both sexes (M=20, F=9 with age ranging between 35-72 years were initiated on 200 mg/day of thalidomide with fortnightly increments of 200 mg to a maximum tolerated dose not exceeding 800 mg/day. All hematological and biochemical parameters were monitored at monthly intervals for one year. STATISTICAL ANALYSIS: Correlation analysis was performed between hemoglobin (Hb, total leukocyte count (TLC, absolute neutrophil count (ANC, platelet count (PC, total proteins (TP, serum albumin and serum globulin on one hand and M protein levels on the other using Pearsons Correlation test by SPSS version 7.5. RESULT: Hb, TLC, ANC, PC and serum albumin levels showed a significant negative correlation with M proteins. A highly significant positive correlation existed between M proteins on one hand and TP and globulin levels on the other. Dryness of skin indicated positive response to therapy. These correlations were found to be significant at the end of one month of therapy in all the above-mentioned parameters except in TLC where it was significant after 2 months of thalidomide therapy. CONCLUSION: Results suggest that sustained efficacy of thalidomide therapy may be amenable to monitoring by these simple, inexpensive and

  9. Association of Apo-E gene polymorphism with biochemical and lipid metabolism parameters in patients with diabetic nephropathy of Hui and Han populations in Gansu Province

    Institute of Scientific and Technical Information of China (English)

    郭茜

    2006-01-01

    Objective To study the association of apolipoprotein (Apo) E gene polymorphism with difference in biochemical metabolism of diabetic nephropathy of Hui and Han populations. Methods ApoE genotype was determined by PCR-RFLP in diabetic patients with or without diabetic nephropathy (DN) and normal peoples in Hui and Han peoples, the related biochemical parameters were simultaneously detected. Results (1) Huis had 3 genotypes, i. e. E2/E3, E3/E3 and E3/E4, and their fre-

  10. Evaluation of sublethal toxicity of zinc and chromium in Eudrilus eugeniae using biochemical and reproductive parameters.

    Science.gov (United States)

    Basha, P Mahaboob; Latha, V

    2016-05-01

    Zinc (Zn) and chromium (Cr) act as essential nutrients; however, they can be toxic at higher concentrations. In this study, earthworm species Eudrilus eugeniae was studied for its sensitivity to sublethal doses of Cr (8 ppm) and Zn (350 ppm) in terms of alterations occurred in oxidative stress indices and reproductive parameters in the testis. A significant (p implied from results that excessive free radical production and inadequate antioxidant defenses have lead to morphological alterations in sperms which sequentially reduced the reproductive rate. PMID:26922588

  11. Proposed parameters of specific rain attenuation prediction for Free Space Optics link operating in tropical region

    Science.gov (United States)

    Suriza, A. Z.; Md Rafiqul, Islam; Wajdi, A. K.; Naji, A. W.

    2013-03-01

    As the demand for higher and unlimited bandwidth for communication channel is increased, Free Space Optics (FSO) is a good alternative solution. As it is protocol transparent, easy to install, cost effective and have capabilities like fiber optics, its demand rises very fast. Weather condition, however is the limiting factor for FSO link. In the temperate region the major blockage for FSO link feasibility is fog. In the tropical region high rainfall rate is expected to be the major drawback of FSO link availability. Rain attenuation is the most significant to influence FSO link availability in tropical region. As for now the available k and α values are developed using data from temperate regions. Therefore, the objective of this paper is to propose new parameters for specific rain attenuation prediction model that represents tropical weather condition. The proposed values are derived from data measured in Malaysia and using methods recommended by ITU-R.

  12. Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)

    Science.gov (United States)

    Feng, G.; Zhuang, P.; Zhang, L.; Kynard, B.; Shi, X.; Duan, M.; Liu, J.; Huang, X.

    2011-01-01

    The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160mgL-1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140mgL-1 group was significantly higher than the level in 80, 100 and 120mgL-1 groups. Aspartate aminotransferase (AST) activity in the 140mgL-1 group was significantly higher than those in the 100 and 120mgL-1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180mgL-1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180mgL-1 were significantly higher than those of 60 and 90mgL-1. Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120mgL-1 and 60-90mgL-1, respectively. Clove oil was a promising alternative to MS-222. ?? 2011 Blackwell Verlag, Berlin.

  13. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries

    Directory of Open Access Journals (Sweden)

    A Kaur

    2012-01-01

    Full Text Available Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.

  14. FPGA-Based Fused Smart Sensor for Dynamic and Vibration Parameter Extraction in Industrial Robot Links

    Science.gov (United States)

    Rodriguez-Donate, Carlos; Morales-Velazquez, Luis; Osornio-Rios, Roque Alfredo; Herrera-Ruiz, Gilberto; de Jesus Romero-Troncoso, Rene

    2010-01-01

    Intelligent robotics demands the integration of smart sensors that allow the controller to efficiently measure physical quantities. Industrial manipulator robots require a constant monitoring of several parameters such as motion dynamics, inclination, and vibration. This work presents a novel smart sensor to estimate motion dynamics, inclination, and vibration parameters on industrial manipulator robot links based on two primary sensors: an encoder and a triaxial accelerometer. The proposed smart sensor implements a new methodology based on an oversampling technique, averaging decimation filters, FIR filters, finite differences and linear interpolation to estimate the interest parameters, which are computed online utilizing digital hardware signal processing based on field programmable gate arrays (FPGA). PMID:22319345

  15. FPGA-Based Fused Smart Sensor for Dynamic and Vibration Parameter Extraction in Industrial Robot Links

    Directory of Open Access Journals (Sweden)

    Rene de Jesus Romero-Troncoso

    2010-04-01

    Full Text Available Intelligent robotics demands the integration of smart sensors that allow the controller to efficiently measure physical quantities. Industrial manipulator robots require a constant monitoring of several parameters such as motion dynamics, inclination, and vibration. This work presents a novel smart sensor to estimate motion dynamics, inclination, and vibration parameters on industrial manipulator robot links based on two primary sensors: an encoder and a triaxial accelerometer. The proposed smart sensor implements a new methodology based on an oversampling technique, averaging decimation filters, FIR filters, finite differences and linear interpolation to estimate the interest parameters, which are computed online utilizing digital hardware signal processing based on field programmable gate arrays (FPGA.

  16. FPGA-based fused smart sensor for dynamic and vibration parameter extraction in industrial robot links.

    Science.gov (United States)

    Rodriguez-Donate, Carlos; Morales-Velazquez, Luis; Osornio-Rios, Roque Alfredo; Herrera-Ruiz, Gilberto; de Jesus Romero-Troncoso, Rene

    2010-01-01

    Intelligent robotics demands the integration of smart sensors that allow the controller to efficiently measure physical quantities. Industrial manipulator robots require a constant monitoring of several parameters such as motion dynamics, inclination, and vibration. This work presents a novel smart sensor to estimate motion dynamics, inclination, and vibration parameters on industrial manipulator robot links based on two primary sensors: an encoder and a triaxial accelerometer. The proposed smart sensor implements a new methodology based on an oversampling technique, averaging decimation filters, FIR filters, finite differences and linear interpolation to estimate the interest parameters, which are computed online utilizing digital hardware signal processing based on field programmable gate arrays (FPGA). PMID:22319345

  17. Effects of 24 h ultra-marathon on biochemical and hematological parameters

    Institute of Scientific and Technical Information of China (English)

    Huey-June Wu; Kung-Tung Chen; Bing-Wu Shee; Huan-Cheng Chang; Yi-Jen Huang; Rong-Sen Yang

    2004-01-01

    AIM: To analyze detailed changes in hematology and biochemistry tests parameters before and after a longdistance race in ultramarathon runners. METHODS: Blood samples of 11 participants were obtained for standard analysis before, immediately after, two days after and nine days after the 2002 International Ultramarathon 24 h Race and the International Association of Ultrarunners (IAU) Asia 24 h Championship. RESULTS: Total bilirubin (BIL-T), direct bilirubin (BIL-D), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased statistically significantly (P<0.05) the race. Significant declines (P<0.05) in red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) were detected two days and nine days d after the race. 2 d after the race, total protein (TP), concentration of albumin and globulin decreased significantly. While BIL, BIL-D and ALP recovered to their original levels. High-density lipoprotein cholesterol (HDL-C) remained unchanged immediately after the race, but it was significantly decreased on the second and ninth days after the race.CONCLUSION: Ultra-marathon running is associated with a wide range of significant changes in hematological parameters, several of which are injury related. To provide appropriate health care and intervention, the man who receives athletes on high frequent training program high intensity training programs must monitor their liver and gallbladder function.

  18. Analysis Of Clinical, Haematological And Biochemical Parameters In Patients With Infectious Mononucleosis

    Directory of Open Access Journals (Sweden)

    Canović Petar

    2015-12-01

    Full Text Available Primary infection with Epstein-Barr virus (EBV usually occurs in early childhood and often does not present clinical symptoms. More than 90% of adults are infected with this virus. A primary infection that occurs in adolescence or adulthood is usually clinically presented as infectious mononucleosis with a triad of symptoms: fever, lymphadenopathy and pharyngitis. Our retrospective study included 51 patients with a median age of 17 (9-23 years and serologically confirmed infectious mononucleosis. All patients with infectious mononucleosis were treated at the Clinic for Infectious Diseases at the Clinical Center in Kragujevac during 2013. We analysed the clinical, haematological and laboratory parameters of patients. The aspartate-aminotransferase levels were increased in 40 patients, with a mean value of 116.24 (±93.22; the alanine-aminotransferase levels were increased in 44 patients, with a mean value of 189.24 (±196.69. Lymphadenopathy was the most common clinical feature upon admission in 49 patients (96%; 38 patients (74.5% had splenomegaly, and 20 (39% had hepatomegaly. Twenty-six patients (51% had leukocytosis with lymphocytosis, while 15 (75% of the 20 who had a normal leukocyte count also had lymphocytosis. In the present study, we updated the clinical, haematological and laboratory parameters, which may lead to the establishment of an accurate diagnosis and promote further treatment of the patients.

  19. The Effect of Deep Water Aqua Treadmill Training on the Plasma Biochemical Parameters of Show Jumpers

    Directory of Open Access Journals (Sweden)

    Anikó Vincze

    2013-09-01

    Full Text Available Aqua treadmill is mainly used for rehabilitation purposes, but research indicates that this equipment could be used for training as well. The few studies performed with aqua treadmill mainly followed lactate and heart rate changes. Therefore, the aim of this study was to test the effect of high water treadmill training on several blood parameters and on the correlations between them. Eight similarly trained Standardbred show jumper horse competing at the same level were selected with age between 6 to 11 years. The horses were subjected to a one week standardized exercise test which included normal training, training with show jumping and aqua treadmill training. The aqua treadmill training consisted of a 10 min walk (filling up, 4.5 km/h, 30 min trot (13 km/h and 4 min walk (emptying, 4.5 km/h. Blood samples were taken from the jugular vein before aqua training, at the completion of each work bout, after drying and after one and two hour rest. Blood plasma were separated and lactate, LDH, CK, AST, glucose, cholesterol, triglyceride, total-bilirubin and cortisol level were determined. In conclusion plasma lactate response itself does not reflect correctly the intensity of workload in high water level aqua training, therefore measurement of several blood parameters is advisable. Further studies needed to understand the relationship of metabolic processes altered due to the effect of partial water submersion.

  20. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

    Directory of Open Access Journals (Sweden)

    Danila Di Majo

    2014-12-01

    Full Text Available In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT] and skeletal and myocardial markers of muscle damage [creatin kinase (CK and creatin kinase MB (CK-MB]. Endurance training, as it implies high oxygen consumption, should increase reactive oxygen species, but it has been shown that exercise leads to increased activation of antioxidant defenses. In fact, serum levels of γ-GT enzyme and total CK were not increased. On the other hand, a statistical significant reduction of CKMB has been observed. There were not variations in hematological parameters. As far as the anthropometric value is concerned, after two months of training there was a change in weight (P<0.0001. Finally, any oxidative and biological stress was highlighted in the middle distance runners but, since this is a preliminary study, it would be of interest to replicate the study on a larger sample.

  1. Prognostic Judgment at Post-Surgery by Biochemical Parameters in Beef Cattle with Left Displaced Abomasum

    Science.gov (United States)

    ICHIJO, Toshihiro; SATOH, Hiroshi; YOSHIDA, Yuki; MURAYAMA, Isao; TAGUCHI, Kiyoshi; SATO, Shigeru

    2014-01-01

    ABSTRACT We examined whether the postoperative prognosis of beef cattle with left displaced abomasum (LDA) can be estimated from changes in laboratory parameters. Preoperatively, beef cattle with LDA showed increases in plasma glucose with decreased serum insulin in the glucose tolerance test compared to non-LDA cattle. Postoperatively, the cattle with LDA were retrospectively divided into two groups, good and bad prognoses. Although plasma glucose concentrations significantly increased either pre- or postoperatively, no difference was noted between the good and bad prognosis groups. Serum insulin concentrations in the bad prognosis group significantly decreased, compared to those in the good prognosis group. These findings suggest that beef cattle with LDA elicit disturbed glucose metabolite pre- and postoperatively, and serum insulin levels may predict their prognoses after surgery. PMID:24998331

  2. A New Data Analysis System to Quantify Associations between Biochemical Parameters of Chronic Kidney Disease-Mineral Bone Disease.

    Directory of Open Access Journals (Sweden)

    Mariano Rodriguez

    Full Text Available In hemodialysis patients, deviations from KDIGO recommended values of individual parameters, phosphate, calcium or parathyroid hormone (PTH, are associated with increased mortality. However, it is widely accepted that these parameters are not regulated independently of each other and that therapy aimed to correct one parameter often modifies the others. The aim of the present study is to quantify the degree of association between parameters of chronic kidney disease and mineral bone disease (CKD-MBD.Data was extracted from a cohort of 1758 adult HD patients between January 2000 and June 2013 obtaining a total of 46.141 records (10 year follow-up. We used an advanced data analysis system called Random Forest (RF which is based on self-learning procedure with similar axioms to those utilized for the development of artificial intelligence. This new approach is particularly useful when the variables analyzed are closely dependent to each other.The analysis revealed a strong association between PTH and phosphate that was superior to that of PTH and Calcium. The classical linear regression analysis between PTH and phosphate shows a correlation coefficient is 0.27, p<0.001, the possibility to predict PTH changes from phosphate modification is marginal. Alternatively, RF assumes that changes in phosphate will cause modifications in other associated variables (calcium and others that may also affect PTH values. Using RF the correlation coefficient between changes in serum PTH and phosphate is 0.77, p<0.001; thus, the power of prediction is markedly increased. The effect of therapy on biochemical variables was also analyzed using this RF.Our results suggest that the analysis of the complex interactions between mineral metabolism parameters in CKD-MBD may demand a more advanced data analysis system such as RF.

  3. Cardio-respiratory, haematological and biochemical parameter adjustments to exercise: effect of a probiotic in horses during training.

    Science.gov (United States)

    Art, T; Votion, D; McEntee, K; Amory, H; Linden, A; Close, R; Lekeux, P

    1994-01-01

    Two randomly distributed groups of thoroughbred horses were compared during a 12-week period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detraining. The horses were trained following the same standardized schedule and were regularly investigated using standardized treadmill exercise tests (SET) of increasing speed. After the first SET and during the whole experimental period, a group of 6 horses received a probiotic (Bioracing) once a day while a group of 5 horses received a placebo. All other conditions were similar for both groups. During each SET, the oxygen uptake, carbon dioxide output, tidal volume (inspired volume), respiratory rate and expired minute volume were obtained using 2 ultrasonic pneumotachographs and a mass spectrometer. All the parameters were the mean of the values calculated during the last 20 s of the SET. Heart rate was continuously measured with a polar horse tester. Venous blood was sampled before and after the test and analyzed for various biochemical parameters. In both groups, training induced significant modification in most of the cardio-respiratory parameters, ie peak oxygen uptake, peak carbon dioxide output, respiratory exchange ratio, ventilation/min to oxygen-uptake ratio and oxygen-uptake to heart-rate ratio. After the 3-week detraining period, most of the values were again similar to the pre-training values in both groups. However, the training-induced modifications of most of the cardio-respiratory parameters occurred earlier and were proportionally greater in the probiotic-treated group than in the control. The respiratory coefficient decreased in the control but not in the treated group. All other parameters changed similarly in both groups. This suggests that Bioracing could modify the physiological effects of training by improving some aerobic metabolic capacities for carbohydrate utilization, but that this effect occurs only during training and not during periods of physical

  4. Connecting parameters optimization on unsymmetrical twin-tower structure linked by sky-bridge

    Institute of Scientific and Technical Information of China (English)

    孙黄胜; 刘默涵; 朱宏平

    2014-01-01

    Based on a simplified 3-DOF model of twin-tower structure linked by a sky-bridge, the frequency response functions, the displacement power spectral density (PSD) functions, and the time-averaged total vibration energy were derived, by assuming the white noise as the earthquake excitation. The effects of connecting parameters, such as linking stiffness ratio and linking damping ratio, on the structural vibration responses were then studied, and the optimal connecting parameters were obtained to minimize the vibration energy of either the independent monomer tower or the integral structure. The influences of sky-bridge elevation position on the optimal connecting parameters were also discussed. Finally, the distribution characteristics of the top displacement PSD and the structural responses, excited by El Centro, Taft and artificial waves, were compared in both frequency and time domain. It is found that the connecting parameters at either end of connection interactively affect the responses of the towers. The optimal connecting parameters can greatly improve the damping connections on their seismic reduction effectiveness, but are unable to reduce the seismic responses of the towers to the best extent simultaneously. It is also indicated that the optimal connecting parameters derived from the simplified 3-DOF model are applicable for two multi-story structures linked by a sky-bridge with dampers. The seismic reduction effectiveness obtained varies from 0.3 to 1.0 with different sky-bridge mass ratio. The displacement responses of the example structures are reduced by approximately 22% with sky-bridge connections.

  5. Effect of Intramuscular Injection of Tobramycin on Some Biochemical Parameters in Blood of Sheep

    Directory of Open Access Journals (Sweden)

    A. Elgerwi

    2012-04-01

    Full Text Available The object of the present study was to assess the possible alterations in blood of sheep that may occur following intramuscular injection of tobramycin. Tobramycin was injected to 10 sheep at a dosage regimen of 5 mg/kg of body weight for 5 successive days. Two types of blood samples (with and without EDTA as an anticoagulant were collected from the jugular vein before and after the antibiotic course. Tobramycin significantly (p<0.05 increased levels of blood urea nitrogen, creatinine, aspartate aminotransferase, alkaline phosphatase, sodium and calcium. On the other hand, it significantly (p<0.05 decreased serum total bilirubin level. Other parameters including, erythrocytic count, packed cell volume, hemoglobin, total and differential leukocytic counts, alanine aminotransferase, G-glutamyltransferse, potassium and phosphorus did not show any significant difference when compared with those of prior-administration samples. These data suggest that tobramycin should be used with care in treatment of sheep with renal impairment; its dosage regimen should be adjusted to avoid its nephrotoxic effect.

  6. Basic biochemical, hematological and hormonal parameters for monitoring the health and nutritional status in athletes

    Directory of Open Access Journals (Sweden)

    Aritz Urdampilleta

    2014-09-01

    Full Text Available Sporting competitions are becoming more demanding in terms of intensity of effort, and this means controlling all aspects that affect athletic performance. Food, hydration and supplementation, before, during and after training or competition directly affect health, body composition, performance and recovery of the athlete. The assessment of nutritional status is required for proper advising of the athlete, through blood tests to control the process of adaptation to training. The aim of this paper is to provide practical tools for dietitiansnutritionists to control the health and nutritional status of athletes, as well as monitoring their adaptation to workloads and competition periods. Performing analytical tests to control of protein metabolism, lipid profile, ions, blood tests and iron metabolism, in addition to review some hormonal parameters, may be of interest in order to observe the potential existence of overtraining states. The correct understanding and interpretation of laboratory tests (under sports doctor’s supervision will be most important and useful for dietitiansnutritionists, performing dietary and nutritional advice to athletes, because it will determine the status of the athlete and propose different individual feeding strategies depending on the training phase and response.

  7. Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites.

    Science.gov (United States)

    Daneshyar, M; Kermanshahi, H; Golian, A

    2009-01-01

    An experiment with 250 one-day-old male broilers (Ross 308) was conducted to investigate the differences of some blood parameters of cold-induced ascitic and healthy broiler chicks in a 6-wk period. The chickens were divided into 2 groups of 5 replicates each. One group of these chickens was raised in normal temperature (NT) treatment and the other in cold temperature (CT) treatment to induce ascites. Mortality was necropsied daily to determine cause of death. At the end of the experiment (wk 6), 5 chickens from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum. Weights of right and left ventricles were determined separately. Average BW gain and average feed intake were measured weekly, and weekly average feed conversion ratio was calculated. Serum glucose, total protein, cholesterol, triglyceride, activity of lactate dehydrogenase, as-partate aminotransferase, and alanine aminotransferase were determined. Throughout the study, the right ventricle-to-total ventricle ratio and total mortality percentage due to ascites of CT-treated birds at the end of experiment was greater (P ascites could affect serum protein and fasting blood sugar of broiler chickens. PMID:19096064

  8. Subclinical hypocalcemia, plasma biochemical parameters, lipid metabolism, postpartum disease, and fertility in postparturient dairy cows.

    Science.gov (United States)

    Chamberlin, W G; Middleton, J R; Spain, J N; Johnson, G C; Ellersieck, M R; Pithua, P

    2013-01-01

    A study was conducted to evaluate the potential association between Ca status at calving and postpartum energy balance, liver lipid infiltration, disease occurrence, milk yield and quality parameters, and fertility in Holstein cows. One hundred cows were assigned to 1 of 2 groups based on whole-blood ionized Ca concentration ([iCa]) on the day of calving [d 0; hypocalcemic [iCa] Cows were blocked based on calving date and parity. Blood samples were collected approximately 14 d from expected calving date (d -14), the day of calving (d 0), and on d 3, 7, 14, 21, and 35 postpartum for measurement of plasma nonesterified fatty acid, iCa, total Ca, glucose, and total and direct bilirubin concentrations, and plasma aspartate aminotransferase and gamma glutamyl transferase activities. Liver biopsies were obtained from a subset of cows on d 0, 7, and 35 for quantification of lipid content. Milk samples were collected on d 3, 7, 14, 21, and 35 postpartum for measurement of somatic cell count and percentages of protein, fat, and solids-not-fat. Data for peak test-day milk yield, services per conception, and days open were obtained from Dairy Herd Improvement Association herd records. Disease occurrence was determined based on herd treatment records. Hypocalcemic cows had significantly higher nonesterified fatty acids on d 0. Hypocalcemic cows also had significantly more lipid in hepatocytes on d 7 and 35 postpartum. However, no statistically significant differences were observed between groups for plasma aspartate aminotransferase and gamma glutamyl transferase activities or total and direct bilirubin concentrations. Milk protein percentage was lower in hypocalcemic cows on d 21 and 35. However other milk quality variables (somatic cell count, milk fat percentage, and solids-not-fat) and milk yield variables (peak test-day milk yield and 305-d mature-equivalent 4% fat-corrected milk yield) did not differ between groups. No differences were observed between groups in the

  9. Effects of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854)

    Science.gov (United States)

    Zhu, Zhiming; Song, Bolan; Lin, Xiaotao; Xu, Zhongneng

    2016-01-01

    This study examines the effect of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb ( Barbonymus schwanenfeldii). Blood samples were taken on days 1, 23 and 45 from control fish and from two training groups maintained at current speeds of 0.06 bl/s (body length per second), 0.66 bl/s, and 1.92 bl/s, respectively. Significantly increased red-blood-cell counts and hematocrit were observed in the post-training groups on days 23 and 45. Significantly increased hemoglobin concentrations were also observed in the 1.92 bl/s group on days 23 and 45. In contrast, values of mean corpuscular volume were significantly lower in the 1.92 bl/s group than in the other groups on day 45. Nitroblue-tetrazolium-positive cells and lysozyme and superoxidase dismutase activities in the plasma increased significantly with increasing training intensity on days 23 and 45. Antibacterial activities were significantly increased in the trained groups compared with the control group on day 23; significantly elevated alkaline phosphatase activity was observed in the 1.92 bl/s groups on day 45. Therefore, training intensities of 0.66 and 1.92 bl/s enhanced the blood oxygen-carrying capability and plasma immune parameters of juvenile tinfoil barbs.

  10. Protective Effects of Bitter Almond Kernel Oil on Some Biochemical Parameters in Brain Tissue of Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Ersin DEMIR

    2013-06-01

    Full Text Available In this study, we aimed to determine possible protective effects of the bitter almond kernel oil extract on the lipid-soluble vitamins, cholesterol, GSH, total protein, MDA and fatty acid levels of brain tissue in the streptozotocin-induced diabetic Wistar rats. The lipid-soluble vitamins, cholesterol and other sterol levels were measured by HPLC, the fatty acid levels were measured by GC, MDA, GSH and total protein levels were measured by UV-Vis spectrophotometer. Whereas the and #945;-tocopherol and GSH levels were decreased in the diabetes (D group, these parameters were protected in the diabetes + almond oil (D+AO group when compared to control (C group. The total protein and MDA levels were increased in the D group, but their levels were not changed in the D+AO group. The palmitic acid and stearic acid levels were increased in the D and D+AO groups. The arachidonic acid and docosahexaenoic acid levels were increased in the D group, but these fatty acid levels were not changed in the D+AO group when compared to the C group. In conclusion, according to our results, bitter almond oil was shown that some positive effects on the biochemical parameters of brain tissue in the diabetic rats. This oil was protected or prevented the GSH and MDA levels in bitter almond oil extract given group, and these values were closed to control group values. [J Intercult Ethnopharmacol 2013; 2(3.000: 127-134

  11. Potential of liquid extracts of Sargassum wightii on growth, biochemical and yield parameters of cluster bean plant

    Institute of Scientific and Technical Information of China (English)

    N Vijayanand; S Sivasangari Ramya; S Rathinavel

    2014-01-01

    Objective:To explore biofertilizing efficiency of seaweed liquid extracts of brown alga Sargassum wightii (S. wightii) on growth, biochemical and yield parameters of Cyamopsis tetragonoloba (C. tetragonoloba). Methods: Seaweeds were made to coarse powder and stock solution was prepared. Different concentrations such as 0.5%, 1.0%, 1.5%, 2.0%, 2.5%and 5.0%were prepared and given as foliar spray. Results: Seaweed Liquid Extract (SLE) at low concentration (1.5%) exhibited promoting effect on growth and yield parameters. Differential responses in the content of photosynthetic pigments, protein, reducing sugar, ascorbic acid and in the activity of nitrate reductase were also observed in the leaves of SLE treated seedlings when compared to untreated seedlings. Higher concentrations (above 1.5%) of SLE were found to show inhibitory effect. Conclusion:The presence of micro and macro nutrients, vitamins, growth hormones and other constituents in the seaweed extract might be very much useful to the crops but their level should be appropriate to enhance growth and productivity. It may be concluded that liquid seaweed extracts could serve as cost effective eco-friendly product for sustainable agriculture.

  12. Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus) experimentally infected with Trichinella zimbabwensis.

    Science.gov (United States)

    La Grange, Louis J; Mukaratirwa, Samson

    2014-01-01

    Fifteen crocodiles were randomly divided into three groups of five animals. They represented high-infection, medium-infection and low-infection groups of 642 larvae/kg, 414 larvae/kg and 134 larvae/kg bodyweight, respectively. The parameters assessed were blood glucose, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT). The humoral immune response to Trichinella zimbabwensis infection was evaluated in all three groups by an indirect ELISA method. The results showed deviations from normal parameters of blood glucose, CPK, LDH, AST and ALT when compared with reported levels in uninfected reptiles. Contrary to studies involving mammals, hypoglycaemia was not observed in the infected groups in this study. Peak values of blood glucose were reached on post-infection (PI) Day 49, Day 42 and Day 35 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of LDH and AST were observed on PI Day 56, Day 49 and Day 42 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of CPK were observed on Day 35 PI in all three groups. Peak ALT values were reached on Day 56 in the high-infection group and on Day 28 PI in both the medium-infection and low-infection groups. No correlations between the biochemical parameters and infection intensity were observed. Peak antibody titres were reached on Day 49 PI in the medium-infection group, and on Day 42 PI in both the high-infection and low-infection groups. Infection intensity could not be correlated with the magnitude of the humoral immune response or time to sero-conversion. Results from this study were in agreement with results reported in mammals infected with other Trichinella species and showed that antibody titres could not be detected indefinitely. PMID:25686027

  13. Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus experimentally infected with Trichinella zimbabwensis

    Directory of Open Access Journals (Sweden)

    Louis J. La Grange

    2014-02-01

    Full Text Available Fifteen crocodiles were randomly divided into three groups of five animals. They represented high-infection, medium-infection and low-infection groups of 642 larvae/kg, 414 larvae/kg and 134 larvae/kg bodyweight, respectively. The parameters assessed were blood glucose, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, aspartate transaminase (AST and alanine transaminase (ALT. The humoral immune response to Trichinella zimbabwensis infection was evaluated in all three groups by an indirect ELISA method. The results showed deviations from normal parameters of blood glucose, CPK, LDH, AST and ALT when compared with reported levels in uninfected reptiles. Contrary to studies involving mammals, hypoglycaemia was not observed in the infected groups in this study. Peak values of blood glucose were reached on post-infection (PI Day 49, Day 42 and Day 35 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of LDH and AST were observed on PI Day 56, Day 49 and Day 42 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of CPK were observed on Day 35 PI in all three groups. Peak ALT values were reached on Day 56 in the high-infection group and on Day 28 PI in both the medium-infection and low-infection groups. No correlations between the biochemical parameters and infection intensity were observed. Peak antibody titres were reached on Day 49 PI in the medium-infection group, and on Day 42 PI in both the high-infection and low-infection groups. Infection intensity could not be correlated with the magnitude of the humoral immune response or time to sero-conversion. Results from this study were in agreement with results reported in mammals infected with other Trichinella species and showed that antibody titres could not be detected indefinitely.

  14. White popular (Populus alba L.) - Litter impact on chemical and biochemical parameters related to nitrogen cycle in contaminated soils

    Energy Technology Data Exchange (ETDEWEB)

    Ciadamidaro, L.; Madejon, P.; Cabrera, F.; Madejon, E.

    2014-06-01

    Aim of study: The aim of this study was to determine the effect of litter from Populus alba on chemical and biochemical properties related to the N cycle in soils with different pH values and trace element contents. We hypothesized that this litter would influence several parameters related to the N cycle and consequently to soil health. Area of study: we collected two reforested contaminated soils of different pH values (AZ pH 7.23 and DO pH 2.66) and a non-contaminated soil (RHU pH 7.19). Materials and methods: Soil samples were placed in 2,000 cm{sup 3} microcosms and were incubated for 40 weeks in controlled conditions. Each soil was mixed with its corresponding litter, and soils without litter were also tested for comparison. Ammonium (NH{sub 4}{sup 4}+-N) and nitrate (NO{sub 3}{sup -} -N) content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN), protease activity, and several chemical properties such as pH, available trace element concentrations (extracted with 0.01 M CaCl{sub 2}) were determined at different times of incubation. Main results: Values of available trace elements did not vary during the incubation and were always higher in acid soil. In neutral soils litter presence increased values of Kjeldahl-N, NO{sub 3} –-N content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN) and protease activity. Presence of trace elements in neutral soils did not alter the parameters studied. However, acidic pH and high content of available trace elements strongly affected NH{sub 4}{sup +}-N and NO{sub 3}{sup -} -N, microbial biomass N and protease activity. Research highlights: Our results showed the negative effect of the acidity and trace element availability in parameters related with the N-cycle. (Author)

  15. Alterations in Somatostatin Cells and Biochemical Parameters Following Zinc Supplementation in Gastrointestinal Tissue of St reptozotocin-Induced Diabetic Rats

    International Nuclear Information System (INIS)

    Chronic hyperglycemia in diabetes is a major causative factor of free radical generation which further leads to many secondary diabetic complications via the damage to cellular proteins, membrane lipids, and nucleic acids. Zinc is an essential trace element in all living systems and plays a structural role in many proteins and enzymes. Somatostatin is known to have inhibitory effects on various gastrointestinal functions. Therefore, we determined somatostatin protein production and secretion levels, and biochemical and light microscopical changes following zinc supplementation in the gastrointestinal tract of streptozotocin (STZ)-diabetic rats. The animals were divided into four groups: Group I: control (untreated) animals; Group II: control animals given zinc sulfate; Group III: diabetic animals; and Group IV: diabetic animals given zinc sulfate. Zinc sulfate was given to the animals by gavage at a daily dose of 100 mg/kg body weight for 60 days. Diabetes was induced by intraperitoneal (i.p.) injection of STZ in a single dose of 65 mg/kg. For histological studies, stomach and duodenum tissues were fixed in Bouin solution and sections stained with Masson’s trichrome and Periodic-Acid-Schiff. Tissue homogenates were used for protein, lipid peroxidation (LPO), glutathione (GSH), and nonenzymatic glycosylation (NEG) analyses. Zinc supplementation to the STZ-diabetic rats revealed the protective effect of zinc on these parameters. Zinc supplementation may contribute to prevent at least some complications of diabetes mellitus

  16. Effects of X-Ray Irradiation Produced by Linear Accelerator on Biochemical Parameters of Irradiated Blood Units

    International Nuclear Information System (INIS)

    Irradiation of blood and cellular blood components is currently practiced in developed and in a few developing countries. The purpose of this procedure is the prevention of transfusion associated graft versus host disease in immunodeficient patients. Dedicated blood irradiators are only available at limited number of facilities in developing countries. Therefore, irradiation remains a limitedly available service due to excessive cost. This study aims to assess the biochemical changes in samples of packed red blood cell units irradiated using X-rays produced by linear accelerator (LINAC), used for radiotherapy, as an efficient and cost-effective solution for hospitals in developing countries instead of a dedicated device. X-rays generated by linear accelerator were used to irradiate red blood cell units. Each unit was divided to two equal portions, one portion was subjected to irradiation and the other portion used as control, irradiation process was performed in 3 minutes.Ph, potassium levels and percentage of hemolysis were analyzed on different storage periods. Progressive increase in potassium and hemolysis percentage and decrease in Ph was noted in the irradiated units. All parameters were within acceptable ranges indicating that the component is suitable for transfusion. From the above results we conclude that packed RBCs (pRBCs) irradiation using radiotherapy linear accelerator is an efficacious and cost-effective solution for most of the hospitals in developing countries

  17. Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats

    International Nuclear Information System (INIS)

    Nutritional Habits have changed significantly and junk foods have become widely popular, in recent years. The present study aimed to shed the light on the effect of potato chips and / or ketchup consumption on some biochemical parameters. Sixty four male and female albino rats were used in the study. Animals were maintained on 0.25 g potato chips/ rat and / or 0.125 g ketchup / rat, 5 days a week for 4 weeks. Potato chips showed the lowest body wt gain in the male rats after 4 weeks but, ketchup modulated this negative effect of the potato chips in the group of male animals fed on potato chips plus ketchup. Potato chips significantly decreased brain serotonin, liver glutathione (GSH) and catalase (CAT) in both sexes; brain dopamine, serum total proteins, albumin, total globulins, α2- and β1-globulins in the females and serum thyroxine (T4) in the male rats. Ketchup apparently affected serum T4 and A / G ratio in both sexes, brain dopamine and liver GSH in the males in addition to brain serotonin, serum total globulins and ?1-globulin in the female rats. Potato chips plus ketchup significantly changed T4, dopamine, GSH, CAT, α1 and α2-globulins in both sexes; serotonin and β1-globulin in the male rats, total proteins and albumin in the females. It could be concluded that potato chips consumption might induce numerous adverse effects in various body organs

  18. Effects of a low-radiotoxicity uranium salt (uranyl acetate) on biochemical and hematological parameters of the catfish, Clarias gariepinus

    Science.gov (United States)

    Al-Ghanim, Khalid A.; Ahmad, Zubair; Al-Kahem Al-Balawi, Hmoud F.; Al-Misned, Fahad; Maboob, Shahid; Suliman, El-Amin M.

    2016-01-01

    Specimens of Clarias gariepinus were treated with lethal (70, 75, 80, 85, 90, and 95 mg/L) and sub-lethal concentrations (8, 12 and 16 mg/L) of uranyl acetate, a low-radiotoxicity uranium salt. The LC 50 value was registered as 81.45 mg/L. The protein and glycogen concentrations in liver and muscles were decreased in the fish exposed to sub-lethal concentrations. The red blood cell (RBC) and white blood cell (WBC) counts, haemoglobin (Hb) concentration and haematocrit (Hct) values were decreased. Different blood indices like mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were negatively affected. Level of plasma glucose was elevated whereas protein was decreased. The level of calcium concentration (Ca) was declined in the blood of exposed fish whereas magnesium (Mg) remains unchanged. The activity level of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) was elevated in exposed fish. These effects were more pronounced in the last period of exposure and in higher concentrations. Results of the present study indicate that uranyl acetate has adverse effects on Clarias gariepinus and causes changes in the biochemical and hematological parameters of the fish.

  19. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats

    Science.gov (United States)

    Piątkowska, Ewa; Kopeć, Aneta; Bieżanowska-Kopeć, Renata; Pysz, Mirosław; Kapusta-Duch, Joanna; Koronowicz, Aneta Agnieszka; Smoleń, Sylwester; Skoczylas, Łukasz; Ledwożyw-Smoleń, Iwona; Rakoczy, Roksana; Maślak, Edyta

    2016-01-01

    Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues and various biochemical parameters as well as mRNA expression of some genes involved in iodine metabolism in Wistar rats. Statistically, a significantly higher iodine level was determined in urine, faeces and selected tissues of rats fed a diet containing biofortified raw carrot as compared to a diet without iodine and a diet containing control cooked carrot. Biofortified raw carrot significantly increased triiodothyronine concentration as compared to animals from other experimental groups. The highest thyroid stimulating hormone level was determined in rats fed control cooked carrots. mRNA expression of selected genes was affected by different dietary treatment in rats’ hearts. Biofortified raw and cooked carrot could be taken into account as a potential source of iodine in daily diets to prevent iodine deficiency in various populations. PMID:27043135

  20. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-09-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

  1. Evaluation of biochemical parameters and genetic markers for association with meat tenderness in South African feedlot cattle.

    Science.gov (United States)

    Frylinck, L; van Wyk, G L; Smith, T P L; Strydom, P E; van Marle-Köster, E; Webb, E C; Koohmaraie, M; Smith, M F

    2009-12-01

    A large proportion of South African feedlot cattle are crossbreds of Brahman (BrX, Bos indicus), and Simmental (SiX, Bos taurus). A sample of 20 grain fed bulls from each of these crossbreeds was used to compare meat quality with that of the small frame indigenous Nguni (NgX, Sanga) by evaluating a variety of biochemical and genetic parameters previously shown to be associated with meat tenderness. Shear force values were generally high (5.6kg average at 14days post mortem), with SiX animals higher than BrX or NgX (P=0.051) despite higher calpastatin:calpain ratio in BrX (P<0.05). Calpain activity and cold shortening were both correlated with tenderness for all classes. The sample size was too small to accurately estimate genotypic effects of previously published markers in the CAST and CAPN1 genes, but the allele frequencies suggest that only modest progress would be possible in these South African crossbreds using these markers. PMID:20416642

  2. Changes In Cortisol And Some Biochemical Parameters In Blood Of Egyptian Buffalo Calves Exposed To Two Successive Summer Seasons

    International Nuclear Information System (INIS)

    Ten male buffalo calves were used in the present study. The animals were housed in semi-open barn. Ration was composed of concentrate mixture and wheat straw according to NRC (1985). Animals were allowed to drink fresh water ad libitum. Plasma cortisol, total protein, albumin, globulin, urea, creatinine, GPT and GOT were determined at the end of thermoneutral and summer heat stress exposure periods. Rectal temperature and respiration rate were recorded daily during both periods whereas body weight was recorded weakly during each period. Summer heat stress induced significant increases in rectal temperature, respiration rate, plasma cortisol, albumin/globulin ratio, plasma urea-N, creatinine, GPT and GOT while total protein, albumin, globulin did not affect by summer heat stress. Body weight was decreased under heat stress in both summer seasons but increased with age. In conclusion, summer heat stress induced alterations in some physiological and biochemical parameters of blood and body weight of male buffalo calves, and the young calves were less tolerant to summer heat stress than the older calves.

  3. Growth Parameters, Photosynthetic Performance, and Biochemical Characterization of Newly Isolated Green Microalgae in Response to Culture Condition Variations.

    Science.gov (United States)

    Jazzar, Souhir; Berrejeb, Nadia; Messaoud, Chokri; Marzouki, Mohamed Néjib; Smaali, Issam

    2016-08-01

    This work aimed to characterize two native microalgal strains newly isolated from South Mediterranean areas and identified as Chlorella sorokiniana ES3 and Neochloris sp. AM2. The growth properties and biochemical composition of these microalgae were evaluated in different culture media (Algal, BG-11, f/2, and Conway). Among the tested media, nitrate- and phosphate-rich Algal medium provided the maximum biomass productivities (85.5 and 111.5 mg l(-1) day(-1) for C. sorokiniana and Neochloris sp., respectively), while the nitrate- and phosphate-deficient f/2 medium resulted in the highest lipid productivities (24.1 and 35.8 mg l(-1) day(-1) for C. sorokiniana and Neochloris sp., respectively). The physiological state of both microalgae was investigated under different light and temperature levels using the pulse amplitude-modulated fluorometry. The better photosynthetic efficiency of C. sorokiniana was obtained at 23 °C with a light saturation of 156 μE m(-2) s(-1), while that of Neochloris sp. was achieved at 15 °C with a light saturation of 151 μE m(-2) s(-1). The analysis of fatty acid profile and biodiesel parameters revealed that C. sorokiniana, cultivated in Algal and f/2 media, can be considered as a suitable candidate for high-quality biodiesel production. PMID:27052210

  4. Correlations between blood lead levels and some physiological and biochemical parameters of nutritional importance in some Nigerian women

    International Nuclear Information System (INIS)

    Lead is one of the common toxic materials widely occurring in the Nigerian environment. Even with the change to unleaded petrol, other significant sources still remain to be addressed. The deleterious impact of lead on human health, is well documented. The effects range from attention-grabbing mass mortality - as happened in Zamfara State recently, to no less serious but grossly neglected neurocognitive and neurodevelopmental effects, including lowered intelligence quotient scores. All these impacts are most serious in children, especially fetuses who receive their burden from their mothers. Ninety percent of lead in most adults resides in the bones with a half-life measured in decades. Therefore, fetuses and breast-fed children in this generation will remain seriously at risk of exposure to damaging lead levels no matter the efforts to rid our external environment of lead in the years to come. In this work, we have investigated the correlation between Blood Lead Levels (BLL) and up to 35 physiological and biochemical parameters of nutritional importance in 62 women of child-bearing age from lIe-lfe, Nigeria. BLL was determined in venous blood using Inductively- coupled plasma Mass Spectrometry. Our results show that BLL significantly correlates with Packed Cell Volume (PCV), Creatine Clearance, and the ratio of Low-density Lipids to High density Lipids in these women. When the subjects were stratified into different Nutritional Status group based on their Body Mass Index, significant correlation was found between BLL and Age but only in Obese subjects.

  5. Influence of thyroid hormones on biochemical parameters of liver function: a case-control study in North Indian population

    Directory of Open Access Journals (Sweden)

    Jayashree Bhattacharjee

    2013-01-01

    Full Text Available Normal level of thyroid hormones is important for normal hepatic function and thyroid dysfunction may modulate metabolic function of liver. The purpose of this study is to determine whether liver function is associated with subclinical and overt hypothyroidism. Thyroid and liver function tests were evaluated in 47 patients with overt (TSH ≥10.0 mIU/L and 77 patients with subclinical hypothyroidism (TSH 6.0-9.9mIU/L and compared with 120 age-matched euthyroid controls. Subjects with overt hypothyroidism had significantly raised serum ALT, AST, ALP and total protein levels as compared to controls whereas subclinical hypothyroid patients had significantly increased levels of serum ALT, ALP and total protein. Further, TSH showed significant positive correlation with AST and ALP values whereas fT3 and fT4 had a negative correlation with AST in overt hypothyroidism. Thus, overt hypothyroid state is associated with significant derangement in biochemical parameters of liver function. Hence, liver function should be regularly monitored in hypothyroid patients.

  6. DIURETIC ACTIVITY AND STUDY OF BIOCHEMICAL PARAMETERS IN THE METHANOL EXTRACT OF HIBISCUS ESCULENTUS (OKRA FRESH FRUITS

    Directory of Open Access Journals (Sweden)

    S Nizamuddin Basha 1

    2011-07-01

    Full Text Available Hibiscus esculentus (Family: Malvaceae is a most common weed in India. The wholeplant is used as medicine. According to Ayurveda,. Hibiscus esculentus is used as in the treatment of constipation, inflammation, anemia, abdominal disorders, urinary tract diseases, general debility. The present study was undertaken to investigate diuretic effect of methanol extract of the Hibiscus esculentus (MEHE in albino rats. Acute oral toxicity study was performed as per OECD guidelines. In acute oral toxicity study, mortality was not observed up to 2000 mg/kg bodyweight. MEHE were administered at the doses of 250 and 500 mg/kg, p.o.Furosemide 20mg per kg body weight was used as positive control in study. The diuretic effect of the extract was evaluated by measuring urine volume, sodium and potassium content. Urine volume is significantly increased at two doses of MEHE 250 & 500 mg/kg body wt in treated rats. The excretion of sodium,Potassium levels was also increased by the MEHE. The diuretic effect of the extract was similar to furosemide. The MEHE had the additional advantage of chloride conserving effect. This study concludes that MEHE produced notable diuretic effect which appeared to be comparable to that produced by the standard diuretic furosemide. The present study provides a quantitative basis for explaining the folkloricuse of Hibiscus esculentus as a diuretic agent. In addition to this different Biochemical parameters were also evaluated.

  7. EFFECT OF DIFFERENT CONCENTRATIONS OF OLIVE PULP ON BODY WEIGHT AND SOME BIOCHEMICAL PARAMETERS IN FRIESIAN CALVES

    International Nuclear Information System (INIS)

    This study was conducted at El-Khaer and El-Barka project, El-Salhya town, El-Sharkia governorate, Egypt. Forty Friesian calves with average body weight of 320 kg were used in this research and divided into four experimental groups. Olive pulp was incorporated in the four groups R1, R2, R3 and R4 at rates of 0, 10, 20 and 30% in calves feeds to replace similar amounts of maize, respectively. Diets were fed for 60 days then body weight was recorded at the beginning and at the end of the experiment and the daily weight gain was calculated for each animal. Blood samples were taken from each animal at the end of the experiment to determine some blood biochemical parameters. The results indicated that the maximum average daily body weight gain was achieved by Friesian calves in R4 (1.38 kg/d). Non-significant changes were recorded in total protein, AST, T4 and lipids but the increase in calcium, phosphorus, urea and creatinine levels was within the normal levels. Feed efficiency was highest in R4 (30% olive pulp) and the feed cost for total weight gain was the lowest value in the same group. It could be recommended to use olive pulp in ruminant ration at 30%, especially when the traditional feed grains are not available and feed costs would be reduced

  8. Effects of Operating Parameters on Measurements of Biochemical Oxygen Demand Using a Mediatorless Microbial Fuel Cell Biosensor

    Directory of Open Access Journals (Sweden)

    Min-Chi Hsieh

    2015-12-01

    Full Text Available The conventional Biochemical Oxygen Demand (BOD method takes five days to analyze samples. A microbial fuel cell (MFC may be an alternate tool for rapid BOD determination in water. However, a MFC biosensor for continuous BOD measurements of water samples is still unavailable. In this study, a MFC biosensor inoculated with known mixed cultures was used to determine the BOD concentration. Effects of important parameters on establishing a calibration curve between the BOD concentration and output signal from the MFC were evaluated. The results indicate monosaccharides were good fuel, and methionine, phenylalanine, and ethanol were poor fuels for electricity generation by the MFC. Ions in the influent did not significantly affect the MFC performance. CN− in the influent could alleviate the effect of antagonistic electron acceptors on the MFC performance. The regression equation for BOD concentration and current density of the biosensor was y = 0.0145x + 0.3317. It was adopted to measure accurately and continuously the BOD concentration in actual water samples at an acceptable error margin. These results clearly show the developed MFC biosensor has great potential as an alternative BOD sensing device for online measurements of wastewater BOD.

  9. Anaerobic co-digestion of commercial food waste and dairy manure: Characterizing biochemical parameters and synergistic effects.

    Science.gov (United States)

    Ebner, Jacqueline H; Labatut, Rodrigo A; Lodge, Jeffrey S; Williamson, Anahita A; Trabold, Thomas A

    2016-06-01

    Anaerobic digestion of commercial food waste is a promising alternative to landfilling commercial food waste. This study characterized 11 types of commercial food wastes and 12 co-digestion blends. Bio-methane potential, biodegradable fraction, and apparent first-order hydrolysis rate coefficients were reported based upon biochemical methane potential (BMP) assays. Food waste bio-methane potentials ranged from 165 to 496 mL CH4/g VS. Substrates high in lipids or readily degradable carbohydrates showed the highest methane production. Average bio-methane potential observed for co-digested substrates was -5% to +20% that of the bio-methane potential of the individual substrates weighted by VS content. Apparent hydrolysis rate coefficients ranged from 0.19d(-1) to 0.65d(-1). Co-digested substrates showed an accelerated apparent hydrolysis rate relative to the weighted average of individual substrate rates. These results provide a database of key bio-digestion parameters to advance modeling and utilization of commercial food waste in anaerobic digestion. PMID:27090232

  10. Hatch ability, growth and some blood biochemical parameters in hatching quails following the exposure of incubated eggs to red laser

    International Nuclear Information System (INIS)

    A total number of 600 Japanese quail eggs were used this study. The eggs were divided into three groups then incubated. Eggs of groups one (G1) and two (G2) were exposed to 30 and 60 minutes, respectively, to the red laser rays (Laser Pointer LD-300) at 0, 7 and 14 days of embryogenesis,while group three (G3) was not treated and served as control. Hatch ability body weight and mortality were recorded weekly for each group. Furthermore, five blood samples were collected from one day old chicks at hatching and at six weeks of age from each group to determine some hematological parameters including RBCs, WBCs, PCV and Hb. Liver enzyme activities (GPT and GOT), kidney function (uric acid and creatinine), cholesterol and total lipids were determined for the 6 weeks old chicks only. The present results indicated that exposure to red to significant increase in hatch ability percentage and promoted early hatch ability and decreased embryonic mortality in treated groups (G1) and (G2) than control (G3). Significant decreases in RBCs counts, cholesterol and total lipids were found in G1 and G2. Exposure of developing embryos to red laser radiation had no significant effect on PCV in one day old hatching chicks, or RBCs and WBCs counts, PCV, GOT, uric acid creatinine in six weeks old birds. At the same time, significant increases in Hb and WBCs counts were occurred in one day old chicks and Hb and GPT in six weeks old birds. It could be concluded from the reported findings that exposure to red laser radiation may affect some factors that accelerate hatch ability and cause changes in some biochemical parameters in Japanese quails

  11. Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men

    Directory of Open Access Journals (Sweden)

    Meng Rao

    2015-01-01

    Full Text Available In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below, motility (P = 0.009 and 0.021, respectively, the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively, total acrosin activity (P = 0.018 and 0.009, respectively, and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively. The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031. We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

  12. Association of Helicobacter pylori IgG antibody with various demographic and biochemical parameters in kidney transplant recipients

    Directory of Open Access Journals (Sweden)

    Azar Baradaran

    2011-01-01

    Full Text Available Few reports are available regarding the promoting factors that affect Helicobacter pylori (H. pylori infection in renal transplant (RTx patients. We report a cross-sectional study that was conducted on a group of stable RTx patients to investigate the relationship of various demographic and biochemical parameters of these patients with serum H. pylori IgG antibody titer as a sign of H. pylori infection. A total of 72 patients who were referred to the clinic for continuing their treatment were enrolled in this study. These patients included 47 males and 25 females. The mean age of the study patients was 44 (±12 years. The mean length of time after they received a transplanted kidney was 67.5 (±42 months (median: 62 months. The mean value of serum H. pylori-specific IgG antibody titer among these patients was 3 (±4.6 U/mL (median: 1 U/mL, and that of intact parathormone (iPTH was 18.4 (±8.2 pg/mL (median: 16.5 pg/mL. The mean serum magnesium (Mg was 1.9 (±0.20 mg/dL (median: 1.9 mg/dL and the mean creatinine clearance was 53 (±11 mL/min (median: 56 mL/min. In this study population, there was no significant difference in the H. pylori IgG antibody titers, serum iPTH, Mg, calcium, alkaline phosphatase and albumin levels as well as body mass index (BMI between males and females or diabetics and non-diabetics. There was no significant relationship between serum H. pylori IgG antibody titers and the age of the patients, BMI, serum Alb, phosphorus, Ca, serum leptin and serum ALP. Significant negative correlation between serum H. pylori IgG antibody titers and serum Mg (r = -0.30, P = 0.01 and serum iPTH (r = -0.25, P = 0.03 was seen. A significant positive correlation was found between serum H. pylori IgG antibody titer and creatinine clearance (r = 0.26, P = 0.02, and a near-significant positive correlation was found with the duration of RTx (r = 0.20, P = 0.08. Our study shows that the correlation of H. pylori IgG antibody titer with some

  13. Three linked nomograms for predicting biochemical failure in prostate cancer treated with radiotherapy plus androgen deprivation therapy

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-Torrecilla, Jose [Hospital General Universitario, Servicio Oncologia Radioterapica- ERESA, Valencia (Spain); Boladeras, Anna [Institut Catala d' Oncologia, S.Oncologia Radioterapica, Hospitalet (Spain); Angeles Cabeza, Maria [Hospital Universitario Doce de Octubre, S.Oncologia Radioterapica, Madrid (Spain); Zapatero, Almudena [Hospital Universitario de la Princesa, S.Oncologia Radioterapica, Madrid (Spain); Jove, Josep [Institut Catala d' Oncologia, S.Oncologia Radioterapica, Badalona (Spain); Esteban, Luis M. [Universidad de Zaragoza, Escuela Universitaria Politecnica de La Almunia, Zaragoza (Spain); Henriquez, Ivan [Hospital Universitari Sant Joan de Reus, S.Oncologia Radioterapica, Reus (Spain); Casana, Manuel; Mengual, Jose Luis [Fundacion Instituto Valenciano de Oncologia, S.Oncologia Radioterapica, Valencia (Spain); Gonzalez-San Segundo, Carmen [Hospital Universitario Gregorio Maranon, S.Oncologia Radioterapica, Madrid (Spain); Gomez-Caamano, Antonio [Hospital Clinico Universitario de Santiago, S.Oncologia Radioterapica, Santiago de Compostela (Spain); Hervas, Asuncion [Hospital Universitario Ramon y Cajal, S.Oncologia Radioterapica, Madrid (Spain); Munoz, Julia Luisa [Hospital Infanta Cristina, S.Oncologia Radioterapica, Badajoz (Spain); Sanz, Gerardo [Universidad de Zaragoza, Departamento de Metodos Estadisticos, Zaragoza (Spain)

    2015-10-15

    Nomograms were established to predict biochemical recurrence (BCR) after radiotherapy (RT) with a low weight of the characteristic variables of RT and androgen deprivation therapy (ADT). Our aim is to provide a new stratified tool for predicting BCR at 4 and 7 years in patients treated using RT with radical intent. A retrospective, nonrandomized analysis was performed on 5044 prostate cancer (PCa) patients with median age 70 years, who received RT - with or without ADT - between November 1992 and May 2007. Median follow-up was 5.5 years. BCR was defined as a rise in serum prostate-specific antigen (PSA) of 2 ng/ml over the post-treatment PSA nadir. Univariate association between predictor variables and BCR was assessed by the log-rank test, and three linked nomograms were created for multivariate prognosis of BCR-free survival. Each nomogram corresponds to a category of the Gleason score - either 6,7, or 8-10 - and all of them were created from a single proportional hazards regression model stratified also by months of ADT (0, 1-6, 7-12, 13-24, 25-36, 36-60). The performance of this model was analyzed by calibration, discrimination, and clinical utility. Initial PSA, clinical stage, and RT dose were significant variables (p < 0.01). The model showed a good calibration. The concordance probability was 0.779, improving those obtained with other nomograms (0.587, 0.571, 0.554) in the database. Survival curves showed best clinical utility in a comparison with National Comprehensive Cancer Network (NCCN) risk groups. For each Gleason score category, the nomogram provides information on the benefit of adding ADT to a specific RT dose. (orig.) [German] Es wurden Nomogramme etabliert, um ein biochemisches Rezidiv (BCR) nach einer Strahlentherapie (RT) vorhersagen zu koennen und den Einfluss der charakteristischen Variablen der RT und der Androgendeprivationstherapie (ADT) dabei moeglichst gering zu halten. Unser Ziel ist es, ein neues stratifiziertes Instrument

  14. Researches Concerning Reference Values Assessment of Serum Biochemical Parameters in some Fish Species from Acipenseridae, Cyprinidae, Esocidae and Salmonidae Family

    Directory of Open Access Journals (Sweden)

    Marioara Nicula

    2010-05-01

    Full Text Available The purpose of this work was to assess reference values of serum biochemical indices (enzymes, metabolites and minerals in some representative fish species belonging to Acipenseridae, Cyprinidae, Esocidae and Salmonidae family in order to establish a baseline data which will serve to monitoring nutritional-metabolic balance and healthy condition of these species from aquatic biocenosis or intensive exploitations. Serum samples were analyzed for glucose, total proteins, albumine, urea nitrogen, creatinine, uric acid, triglycerides, cholesterol, ALT, AST, GGT, alkaline phosphatase, amylase, total bilirubin, Ca, P, Mg, Fe, using a FullyVet automated chemical analyser. The obtained results were compared with those from other papers regarding serum biochemical profile of fresh water fish species. Serum biochemical reference intervals were as follows: glucose, 28.41±0.80–64.00±1.41 mgdL-1; total proteins, 2.78±0.21-4.05±0.20 gdL-1; albumine, 0.67±0.12–1.59±0.11 gdL-1; urea nitrogen, 12.16±0.78–18.30±0.27 mgdL-1; creatinine, 0.06±0.01–0.27±0.00 mgdL-1; uric acid, 1.00±0.00–1.66±0.09 mgdL-1; triglycerides, 46.00±1.29– 351.3±12.3 mgdL-1; cholesterol, 123.00±2.12–198.00±0.91±mgdL-1; ALT, 15.00±0.91–32.00±1.29 IU L-1; AST, 30.00±0.91–92.00±1.47 IU L-1; GGT, 4.00±0.00–6.33±0.46 IU L-1; alkaline phosphatase, 60.33±1.20–109.50±3.05 IU L-1; amylase, 28.08±0.93 – 36.00±0.91 IU L-1; total bilirubin, 0.00±0.00–0.03±0.00 mgdL-1; Ca, 7.63±0.40– 12.36±0.50 mgdL-1; P, 11.83±0.35-30.48±0.26 mgdL-1; Mg, 1.80±0.14–3.88±0.21mgdL-1; Fe, 57.60±3.48–120.00± 1.08 gdL-1. The wide intra- and interspecific variability of our data requires subsequent studies of the endo- and exogenous factors (living condition, season, age, gender, origin, breeding system, physiological and nutritional status, genetic of each individual, etc. that can induce variations of the propound parameter.

  15. EFFECTS OF SOYBEAN MEAL BASED DIET ON GROWTH PERFORMANCE AND HEMOLYMPH BIOCHEMICAL PARAMETERS OF NARROW-CLAWED CRAYFISH (ASTACUS LEPTODACYLUS ESCHSCHOLTZ, 1823

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2013-01-01

    Full Text Available Like other crustaceans, narrow-clawed crayfish (Astacus leptodacylus Eschscholtz, 1823 can change its diet to the available food during varied life cycles. Diet alteration can affect different biological indices of this species, therefore this study aims at studying changes in growth indices, hemolymph biochemical parameters and biochemical quality of its carcasses, which might occur during diet change of crayfish. The purpose of this experiment was to evaluate the effects of partial replacement of fish meal with soybean meal on growth performance, carcass quality and hemolymph biochemical parameters of narrow-clawed crayfish. 90 healthy adult narrow-clawed crayfish (W=35.50±4.05 g; TL=16.96±1.92 cm were randomly distributed into 9 fiberglass tanks (200 L and were fed for 45 days with three varied diets including: commercial shrimp diet, fishmeal-based diet (A and soybean meal-based diet (B. The results show that changing the diet from animal protein to plant protein caused a significant decrease in the cholesterol and triglyceride levels in hemolymph, as well as carcass fat of the crayfish nourished with B diet when compared with the crayfish fed with A diet. No significant changes of hemolymph levels of glucose, AST and ALT were found between different treatments during this experimental period. In conclusion, it was found that though growth performance reduced, the increased rate of soybean meal in diet from 0.0% to 76% had no adverse effects on biochemical parameters.

  16. Genetic variability in different biochemical traits and their relationship with yield and yield parameters of cotton cultivars grown under water stress conditions

    International Nuclear Information System (INIS)

    Water scarcity is an important factor limiting cotton production worldwide particularly in Pakistan. To identify drought tolerant genotypes, it is vital to understand their genetic variation for different biochemical traits under water limited conditions. In the present study, 24 genotypes of cotton (Gossypium hirsutum L.) were evaluated under two irrigation regimes viz., well watered (W1) and limited water (W2) conditions. Before physiological maturity, cotton leaves were collected and analyzed for nitrate and nitrite reductase activities, and total free amino acids. At maturity, data regarding yield and yield parameters were recorded. Significant reduction in case of all the activities of nitrate and nitrite reductase, and yield parameters was observed under W2 condition in all the genotypes; however, total free amino acids were substantially increased under W 2 condition. Correlation between the yield parameters of cotton and biochemical traits was determined. Non-significant correlation between nitrate reductase activity and yield parameters was observed under limited water condition. The genotypes evaluated exhibited decrease in the activities of nitrate and nitrite reductase whereas total free amino acids accumulation was higher under drought conditions that showed comparatively higher yield. This study shows that these biochemical traits were regulated genetically and environmentally in the tested cotton genotypes. It was concluded that these biochemical traits can be used as biochemical markers for screening cotton germplasm for drought tolerance as well as for evolving high yielding drought tolerant varieties of this crop. The findings are useful in bridging plant biochemistry and molecular biology for identifying and selecting genes involved in conferring drought tolerance in cotton. (author)

  17. Detection of vitamin B12 levels with the aid of some hematological and biochemical parameters that are more sensitive

    Directory of Open Access Journals (Sweden)

    Süheyl Asma

    2010-09-01

    Full Text Available Vitamin B12 deficiency is a health problem, which is usually seen in elderly patients and results in hematological and neurological disorders. In this study, we aimed to investigate the serum levels of vitamin B12 could represent the real tissue levels with con-sidering some hematological and biochemical parameters. 128 patients whose vitamin B12 levels have been detected to be low and their ages are ranged from 16 to 90, have been investigated. Fifty three of them were females. Complete blood counts, antiparietal antibody(APA, homocysteine levels, peripheral blood smear evaluations and findings of gastroduodenoscopy were assessed. Mean volume of erythrocytes has been found as 107 fl in patient group (group 1, in which vitamin B12 level has been detected lower than 60 pg/mL. Thehigher homocysteine values in 85% cases, neutrophil hyper-segmentation in 82%, atrophic gastritis in 53 % and APA positivity in 47 % of cases have been detected. The average of agehas been detected as 65 in this group. High level of homocysteine has been present in 44 % of cases and MCV has been 91 fl in group 2, in which the vitamin B12 levels are between 61–100 pg/ mL. In group ( group 3, in which vitamin levels are between 101 – 140 pg /mL,elevated homocysteine levels in 27 % cases and MCV as 89 fl have been detected. In group 4, in which vitamin levels are between 141–178 pg/mL, homocysteine elevation in 16 % of cases and MCV as

  18. Risks of a fructose rich soft drink consumption on some biochemical parameters in Balb/c mice

    International Nuclear Information System (INIS)

    Consumption of soft drinks, in particular carbonated beverages, has markedly increased in the last two to three decades. In fact, the carbonated beverages are the most popular refreshments among most of the world's population. The aim of this study was to assess the body weight gains and analyze some biochemical and hematological parameters in male and female Balb/c mice. Animals were provided with diluted coca cola (fructose rich soft drink) for 6 weeks and were matched against corresponding control animals. The control animals were maintained on tap water ad libitum and coca cola was diluted v/v by tap water. The results revealed that the percentages of body weight gains of male and female control animals were significantly higher than the corresponding mice provided with coca cola. Hemoglobin content (Hb, g/100ml blood), erythrocytes (RBCs), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), leucocytes (WBCs), granulocytes and blood platelet counts were significantly decreased in the groups of mice treated with the soft drink, compared to the control groups. However, significant increases were observed in lymphocytes in the treated groups. Consumption of the soft drink (coca cola) in both male and female animal groups exhibited significant increases of total cholesterol, triglycerides, low density lipoprotein-c and atherogenic index. Levels of serum calcium significantly decreased in both male and female treated mice than the corresponding control groups. Consumption of coca cola did not affect the levels of T3. This study suggests that consumption of high fructose soft drink may lead to many health problems

  19. Effects of the surfactant polyoxyethylene amine (POEA) on genotoxic, biochemical and physiological parameters of the freshwater teleost Prochilodus lineatus.

    Science.gov (United States)

    Navarro, Claudia D C; Martinez, Claudia B R

    2014-09-01

    The surfactant polyoxyethylene amine (POEA) is added to several formulations of glyphosate herbicides that are widely used in agriculture and can contaminate aquatic ecosystems. In the present study, an integrated approach examining genotoxic, biochemical and physiological parameters was employed to evaluate acute effects of POEA on the Neotropical fish Prochilodus lineatus. Juvenile fish were exposed to 0.15 mg·L(-1) (POEA 1), 0.75 mg·L(-1) (POEA 2) and 1.5 mg·L(-1) (POEA 3) of POEA or only water (CTR), and after 24h exposure samples of blood and liver were taken. Compared with CTR, liver of fish exposed to POEA 2 and POEA 3 showed increased activity of 7 ethoxyresorufin-O-deethylase and increased content of glutathione, whereas the activity of glutathione-S-transferase was diminished. On the other hand, fish of the group POEA 1 showed an increase in the activity of superoxide dismutase and in the occurrence of lipid peroxidation. Fish exposed to POEA 3 presented increased hepatic activity of glutathione peroxidase and reduced plasma cortisol. The exposure to POEA at all concentrations tested caused an increase in plasma lactate and a decrease in the hepatic activity of catalase, in the number of red blood cells and in hemoglobin content. The comet assay used for analyzing DNA damage in blood cells indicated the genotoxicity of the surfactant at all concentrations tested. Taken together these results show that POEA can cause effects at various levels, such as hemolysis, DNA damage and lipid peroxidation, which are directly related to an imbalance in the redox state of the fish. PMID:24955954

  20. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal.

    LENUS (Irish Health Repository)

    2012-02-01

    Abstract Background: The aim of the study was to define the analytical and diagnostic performance of the Labor Diagnostica Nord (LDN) 2-Met plasma ELISA assay for fractionated plasma metanephrines in the biochemical diagnosis of phaeochromocytoma. Methods: The stated manufacturer\\'s performance characteristics were assessed. Clinical utility was evaluated against liquid chromatography tandem mass spectrometry (LC-MS\\/MS) using bias, sensitivity and specificity outcomes. Samples (n=73) were collected from patients in whom phaeochromocytoma had been excluded (n=60) based on low probability of disease, repeat negative testing for urinary fractionated catecholamines and metanephrines, lack of radiological and histological evidence of a tumour and from a group (n=13) in whom the tumour had been histologically confirmed. Blood collected into k(2)EDTA tubes was processed within 30 min. Separated plasma was aliquoted (x2) and frozen at -40 degrees C prior to analyses. One aliquot was analysed for plasma metanephrines using the LDN 2-Met ELISA and the other by LC-MS\\/MS. Results: The mean bias of -32% for normetanephrine (ELISA) when compared to the reference method (LC-MS\\/MS) makes under-diagnosis of phaeochromocytoma likely. The sensitivity of the assay (100%) was equal to the reference method, but specificity (88.3%) lower than the reference method (95%), making it less than optimum for the biochemical diagnosis of phaeochromocytoma. Conclusions: Plasma-free metanephrines as measured by Labor Diagnostica Nord (LDN) 2-Met ELISA do not display test characteristics that would support their introduction or continuation as part of a screening protocol for the biochemical detection of phaeochromocytoma unless the calibration problem identified is corrected and other more accurate and analytically specific methods remain unavailable.

  1. Toxicity of Neem Leaf Extracts (Azadirachta indica A. Juss on Some Haematological, Ionoregulatory, Biochemical and Enzymological Parameters of Indian Major Carp, Cirrhinus mrigala

    Directory of Open Access Journals (Sweden)

    M. Saravanan

    2011-10-01

    Full Text Available In the present study, the median lethal concentration (LC 50 of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT and glutamate pyruvate transaminase (GPT activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in environmental biomonitoring of neem based products in freshwater environment.Keywords: Azadirachta indica, Acute toxicity, Cirrhinus mrigala, Haematology, Ion regulation, Biochemical and Enzymological parameters.

  2. The dynamics of biochemical parameters in blood of clinically healthy holstein cows from day 5 before to day 60 after calving

    Directory of Open Access Journals (Sweden)

    Irena Celeska

    2015-10-01

    Full Text Available The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and BHBA. Milk production and body condition score were also estimated. Obtained results showed that cows were exposed to mild to marked metabolic distress. Energy status was changed due to increased values of NEFA and BHBA and decreased value of glucose after calving. Protein concentrations were increased at day 10 after calving, despite the decrease of the level of albumin. Urea concentrations before and after calving were within physiological range indicating an optimal protein diet. Increased values of total bilirubin at day 5 after calving indicated liver increased activity. Lipid status presented by triglycerides and total cholesterol revealed no differences in blood concentrations. Milk production was highest at day 30 after calving. BCS were highest in dry cows, thereafter they declined and recovered at day 60 after calving. In conclusion, biochemical parameters can be used as relevant indicators of metabolic distress in cows around calving with milk and BCS recording as aside parameters. Changes in some biochemical parameters indicate liver increased activity and metabolic stress, that could lead to decreased milk production, impaired reproductive performance and, finally, to illness.

  3. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats.

    Science.gov (United States)

    Beck, W; Gobatto, C

    2016-03-01

    Nocturnal rodents should be assessed at an appropriate time of day, which leads to a challenge in identifying an adequate environmental light which allows animal visualisation without perturbing physiological homeostasis. Thus, we analysed the influence of high wavelength and low intensity light during dark period on physical exercise and biochemical and haematological parameters of nocturnal rats. We submitted 80 animals to an exhaustive exercise at individualised intensity under two different illuminations during dark period. Red light (> 600 nm; haematological and biochemical conditions, demonstrating that EI alone can influence physiological parameters and jeopardise result interpretation. SI promotes normal physiological conditions and greater aerobic tolerance than EI, showing the importance of a correct illumination pattern for all researchers that employ nocturnal rats for health/disease or sports performance experiments. PMID:27030633

  4. The usefulness of whole body bone mineral densitometry in the osteopenia of preterm infants: comparison with the wrist radiography and biochemical parameters

    International Nuclear Information System (INIS)

    To evaluate the usefulness of whole body bone mineral densitometry in the diagnosis of frequent osteopenia of preterm infants by comparison with the wrist radiographs and biochemical parameters. From January 1995 to January 1996, we obtained whole body bone mineral density(BMD) studies using dual energy X-ray absorptiometry(DXA) and wrist radiographs of 39 preterm infants. They were divided into three groups according to birth weight, under 1500g, 1501g to 2000g and above 2000g, and four grades of skeletal change, as seen on wrist radiography, according to the scoring method of Koo et al. Groups of birth weight and grades of skeletal change were then correlated with whole body BMD and biochemical parameters. For comparison, normal data were obtained from 13 infants born at full term. Data were analyzed by one way analysis of variation(ANOVA) and correlation and regression analysis. A p-value of less than 0.05 was considered significant. Whole body BMDs were significantly lower in the more premature and smaller birth weight infants(r=0.77, p=0.0000), and in the higher grade of skeletal change(r-0.5276, p=0.0000). Aggravated skeletal changes were found in infants with lower birth weight(r= -0.3822, p=0.01). Interobserver variation in grading skeletal change was 42.9%, and intraobserver variation was 18.4%. Biochemical parameters such as serum calcium, phosphate, alkaline phosphatase, parathromone, calcitonin and 25-hydroxy-vitamine D did not vary significantly according to either birth weight or skeletal change(p > 0.05). Premature osteopenia is more effectively diagnosed by measuring whole body BMD using DXA than by grading radiographical skeletal change or by biochemical parameters

  5. Acute Effects of Hexavalent Chromium on Survival, Oxygen Consumption, Hematological Parameters and Some Biochemical Profiles of the Indian Major Carp, Labeo rohita

    OpenAIRE

    Vutukuru, S. S.

    2005-01-01

    An attempt has been made in the present investigation to determine the acute toxicity of hexavalent chromium and its toxicological effects on survival, physiological, hematological and biochemical parameters of the widely consumed Indian major carp, Labeo rohita. Short-term acute toxicity tests were performed adopting renewal bioassay technique (USEPA, 1975) over a period of 96h, using different concentrations of potassium dichromate to the fish and the 96 h LC50 value was found to be 111.45 ...

  6. Dosimetric parameters as predictive factors for biochemical control in patients with higher risk prostate cancer treated with Pd-103 and supplemental beam radiation

    International Nuclear Information System (INIS)

    Purpose: To analyze the role of dosimetric quality parameters in maximizing cancer eradication in higher risk prostate cancer patients treated with palladium (Pd)-103 and supplemental beam radiation. Methods: One-hundred-seventy-nine patients treated with Pd-103 and supplemental beam radiation, with minimum 2 years follow-up prostate-specific antigen (PSA) values and posttreatment computed tomography scans were analyzed. Dosimetric parameters included the V100 (percent of the postimplant volume covered by the prescription dose), the D90 (the minimum dose that covered 90% of the post implant volume), and the treatment margins (the radial distance between the prostatic edge and the prescription isodose). Treatment margins (TMs) were calculated using premarket software. Results: Freedom from biochemical failure was 79% at 3 years, with 92 of the 179 patients (51%) followed beyond 3 years. In comparing patients who did or did not achieve biochemical control, the most striking differences were in biologic factors of pretreatment PSA and Gleason score. The V100, D90, and average TM all showed nonsignificant trends to higher values in patients with biochemical control. In multivariate analysis of each of the three dosimetric parameters against PSA and Gleason score, TM showed the strongest correlation with biochemical control (p = 0.19). Conclusions: For patients with intermediate and high-risk prostate cancer treated with Pd-103 brachytherapy and external beam radiation, biologic factors (PSA and Gleason score) were the most important determinants of cancer eradication. However, there is a trend to better outcomes among patients with higher quality implant parameters, suggesting that attention to implant quality will maximize the likelihood of cure

  7. Supplementation with L-Glutamine and L-Alanyl-L-Glutamine Changes Biochemical Parameters and Jejunum Morphophysiology in Type 1 Diabetic Wistar Rats

    OpenAIRE

    da Rosa, Carlos Vinicius D.; Azevedo, Silvia C. S. F.; Bazotte, Roberto B.; Peralta, Rosane M.; Buttow, Nilza C.; Maria Montserrat D Pedrosa; Vilma A F de Godoi; Maria Raquel M Natali

    2015-01-01

    We evaluated the effects of the supplementation with L-glutamine and glutamine dipeptide (GDP) on biochemical and morphophysiological parameters in streptozotocin-diabetic rats. For this purpose, thirty animals were distributed into six groups treated orally (gavage) during thirty days: non diabetic rats (Control) + saline, diabetic + saline; Control + L-glutamine (248 mg/kg), Diabetic + L-glutamine (248 mg/kg), Control + GDP (400 mg/kg), Diabetic + GDP (400 mg/kg). Diabetes was induced by an...

  8. Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows

    OpenAIRE

    Akalın Pınar Peker; Ataseven Veysel Soydal; Fırat Doğan; Ergün Yaşar; Başpınar Nuri; Özcan Oğuzhan

    2015-01-01

    The aim of this study was to determine the ceruloplasmin (Cp) and vitamin C concentrations, the total antioxidant status (TAS), and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV). Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL), whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST) (P = 0.003) and Cp concentrations (P = 0.03) decreas...

  9. Protective effect of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaei, S; Nikzad, H; Taghizadeh, M; Tameh, A A; Taherian, A; Moravveji, A

    2014-10-01

    Cancer treatment with cyclophosphamide (CP) may result in reproductive toxicity as one of its side effects. The pumpkin seed is a rich natural source of antioxidant. We have assessed the possible protective efficacy of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology of CP-treated rats. Male adult Wistar rats were categorised into four groups. Group 1 served as control and received intraperitoneal (IP) injection of isotonic saline solution. Group 2 rats were treated with CP by IP injection in a single dose of 100 mg/kg body weight, only once. Group 3 and 4 received CP plus 300 and 600 mg/kg pumpkin seed extract respectively. Six weeks after treatment, sperm characteristics, biochemical parameters and histopathological changes were examined. Results showed that, sperm characteristics in CP-treated rats were significantly decreased. Biochemical analysis results showed that the co-administration of 300 mg pumpkin seed extract could increase the total antioxidant capacity (TAC) level significantly. In CP-treated rats, histopathological changes such as vacuolisation, disorganisation and separation of epididymal epithelium were observed as well. Interestingly, pumpkin seed extract could improve the above-mentioned parameters remarkably in CP-treated rats. Our findings indicated that pumpkin seed extract might be used as protective agent against CP-induced reproductive toxicity. PMID:24124763

  10. PROPOSED MODIFICATIONS OF K2-TEMPERATURE RELATION AND LEAST SQUARES ESTIMATES OF BOD (BIOCHEMICAL OXYGEN DEMAND) PARAMETERS

    Science.gov (United States)

    A technique is presented for finding the least squares estimates for the ultimate biochemical oxygen demand (BOD) and rate coefficient for the BOD reaction without resorting to complicated computer algorithms or subjective graphical methods. This may be used in stream water quali...

  11. Changes in Some Biochemical Parameters and Somatic Cell Counts in the Milk of Buffalo and Cattle Suffering from Mastitis

    OpenAIRE

    Riaz Hussain§, Muhammad Tariq Javed and Ahrar Khan*

    2012-01-01

    The study was conducted on a total of 592 buffaloes and 453 cattle in their different stages of lactation to investigate the biochemical changes occurring in milk due to mastitis. California Mastitis Test (CMT) was used to diagnose the mammary gland infection. The results revealed significant (P

  12. The Toxicological effects of fenitrothion and vitamin E as antioxidant agent on the biochemical, cytogenetic and histopathological parameters of white rats

    Directory of Open Access Journals (Sweden)

    Fouad Abdel Reheim* Awad Abbas Ragab * Fatma.M. Hammam

    2008-12-01

    Full Text Available Background: The use of pesticides has been increased considerably nowadays compared to the past. The hazards of using such chemical compounds have been accentuated by the sharp rise of their use in agriculture, industry, by householders and governments. Exposure to organophosphorus insecticides (OPI in agriculture is one of the occupational hazards. Fenitrothion is one of the most important OPI. The major object of the present study was to evaluated the toxicological (biochemical, mutagenic and histopathological effects of tested insecticide "fenitrothion" alone or combined to vitamin E as an antioxidant agent to decrease their toxic effect. Material and Method male albino rats were tested orally for 30 days, three doses of fenitrothion were used in absence and presence of vitamin E (1/20, 1/40 and 1/80 LD50. Results the obtained data showed marked changes in biochemical parameter, highly inhibition of AchE activity; highly significant increase in the frequency of micronucleus (PCEM in rat bone marrow cells at all doses of fenitrothion alone or combined to vitamin compared to control group. Also, the histopathological examination of liver and kidney tissues revealed high alternation in these tissues corresponding to biochemical changes. Conclusion From these results we concluded that fenitrothion exert biochemical, mutagenic and histopathological effects in white rats. In addition, vitamin E has mild role in alleviating these toxicological effects

  13. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata) and Wheat (Triticum aestivum) Seedlings

    OpenAIRE

    Mobin Afzal; Simeen Mansoor

    2012-01-01

    Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bea...

  14. The effect of sodium valproate on the biochemical parameters of reproductive function in male albino Wistar rats

    OpenAIRE

    Vijay P.; Yeshwanth R; Bairy K

    2008-01-01

    Objective: To assess the effects of sodium valproate on intratesticular testosterone and lactic dehydrogenase level in rats. Methods: Male Wistar rats (12 weeks old) were treated with sodium valproate and sacrificed at the end of the 2 nd , 4 th , 5 th , 7 th , 10 th and 15 th week, after the last exposure to sodium valproate. The testes were removed, weighed and processed for biochemical analysis. Results: The intratesticular testosterone level was significantly (P< 0.001) reduced...

  15. Dikegulac-Sodium Effect on Micropropagation and Biochemical Parameters in the Cherry Rootstocks CAB-6P and Gisela 6

    OpenAIRE

    SARROPOULOU, VIRGINIA; DIMASSITHERIOU, Kortessa; Therios, Ioannis

    2014-01-01

    The effects of dikegulac-sodium (DS) alone and in combination with benzyladenine (BA) οn the morphogenetic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus x Prunus canescens) were investigated. In the CAB-6P rootstock, DS did not promote shoot proliferation whereas its application at 40, 120 and 150 μΜ suppressed shoot length. In the Gisela 6 rootstock, the number of shoots per explant and shoot proliferation rate were greater with 80...

  16. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2014-08-01

    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint ( P > 0.05). A significant interaction between dietary CrPic and P.mint on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P diet including both CrPic and P.mint. Plasma chromium (Cr) content increased significantly ( P diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  17. Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaie, Somaieh; Nikzad, Hossein; Mahabadi, Javad Amini; Taghizadeh, Mohsen; Azami-Tameh, Abolfazl; Taherian, Aliakbar; Sajjadian, Seyyed Mohammad Sajjad; Kamani, Mehran

    2016-09-01

    Reproductive toxicity is one of the side effects of cyclophosphamide (CP) in cancer treatment. Pumpkin seeds and Zingiber officinale are natural sources of antioxidants. We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats. Male adult Wistar rats were divided randomly into six groups. Group 1, as a control, received an isotonic saline solution injection intraperitoneally (IP). Group 2 were injected IP with a single dose of CP (100 mg/kg) once. Groups 3 and 4 received CP plus 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract (50:50). Groups 5 and 6 received only 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract. Six weeks after treatment, sperm characteristics, histopathological changes and biochemical parameters were assessed. In CP-treated rats, motile spermatozoa were decreased, and abnormal or dead spermatozoa increased significantly (P < 0.001) but administration of the mixed extract improved sperm parameters. Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. Biochemical analysis showed that the administration of combined extracts could increase the total antioxidant capacity (TAC) level significantly in groups 3, 4, 5 and 6. Interestingly, the mixed extract could decrease most of the side effects of CP such as vacuolization and separation of epididymal tissue. Our findings indicated that the combined extracts might be used as a protective agent against CP-induced reproductive toxicity. PMID:26714700

  18. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2014-08-01

    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint ( P > 0.05). A significant interaction between dietary CrPic and P.mint on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P < 0.05) in broilers fed the diet including both CrPic and P.mint. Plasma chromium (Cr) content increased significantly ( P < 0.05) in birds fed the CrPic-included diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  19. The Effects of Bisphosphonates Used Continually or Intermittently on Fractures, Bone Mineral Density and Biochemical Parameters in Osteoporotic Patients - Original Investigation

    Directory of Open Access Journals (Sweden)

    Alev Çevikol

    2010-04-01

    Full Text Available Aim: The aim of this study was to evaluate the effects of bisphosphonates on new fracture development, bone mineral density and biochemical parameters in osteoporotic patients who were treated with these drugs for 5 years. Material and Methods: Thirty nine patients from our osteoporosis outpatient clinic, using bisphosphonates treatment for 5 years were included in this retrospective study. The patients were questioned in terms of demographic features, osteoporosis risk factors, spine and total hip BMD scores measured during the diagnosis and the last follow-up, duration of bisphosphonates use, adverse-effect profile and compliance to the treatment. Serum calcium, phosphorus, alkaline phosphatase levels and 24 houred-urine calcium level were examined. Patients were divided into 2 groups as the patients who were using bisphosphonates continually after diagnosis were group 1 and the patients left using bisphosphonates for some time because of several reasons treated intermittently were group 2. Results: After the diagnosis, 11 (28.2% patients received bisphosphonate treatment continually (Group 1 while 28 (71.8% used the treatment intermittently (Group 2 for 5 years. The break in bisphosphonate use in Group 2 was 1.25±0.63 years. No statistical differences were determined between the 2 groups with respect to DEXA measurement, biochemical parameters or new fracture development identified clinically (p>0.05. Conclusion: Efficacy of bisphosphonates on new fracture development identified clinically, biochemical parameters and DEXA measurement was sustained in patients using bisphosphonates regularly for 5 years, even when treatment was interrupted for approximately 1.5 years. (From the World of Osteoporosis 2010;16:1-8

  20. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish. PMID:27245867

  1. Prognostic value of metabolic parameters and clinical impact of {sup 18}F-fluorocholine PET/CT in biochemical recurrent prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Colombie, M.; Bailly, C.; Rusu, D.; Rousseau, N. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Campion, L. [ICO Cancer Center, Statistics, Saint-Herblain (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, T. [Urologic Clinic Nantes-Atlantis, Saint Herblain (France); Mathieu, C. [University Hospital, Nuclear Medicine, Nantes (France); Ferrer, L. [ICO Cancer Center, Physics, Saint-Herblain (France); Kraeber-Bodere, F. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); University Hospital, Nuclear Medicine, Nantes (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, C. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France)

    2015-11-15

    To evaluate the therapeutic impact of {sup 18}F-fluorocholine (FCH) PET/CT in biochemical recurrent prostate cancer (PC) and to investigate the value of quantitative FCH PET/CT parameters in predicting progression-free survival (PFS). This retrospective study included 172 consecutive patients with PC who underwent FCH PET/CT for biochemical recurrence. Mean rising PSA was 10.7 ± 35.0 ng/ml. Patients with positive FCH PET were classified into three groups: those with uptake only in the prostatic bed, those with locoregional disease, and those with distant metastases. Referring physicians were asked to indicate the hypothetical therapeutic strategy with and without the FCH PET/CT results. Clinical variables and PET parameters including SUVmax, SUVpeak, SUVmean, total lesion choline kinase activity (TLCKA) and standardized added metabolic activity (SAM) were recorded and a multivariate analysis was performed to determine the factors independently predicting PFS. In 137 of the 172 patients, the FCH PET/CT scan was positive, and of these, 29.9 % (41/137) had prostatic recurrence, 42.3 % (58/137) had pelvic lymph node recurrence with or without prostatic recurrence, and 27.7 % (38/137) had distant metastases. The FCH PET/CT result led to a change in treatment plan in 43.6 % (75/172) of the 172 patients. Treatment was changed in 49.6 % (68/137) of those with a positive FCH PET/CT scan and in 20 % (7/35) of those with a negative FCH PET/CT scan. After a median follow-up of 29.3 months (95 % CI 18.9 - 45.9 months), according to multivariate analysis age <70 years, SAM ≥23 and SUVmean ≥3 were parameters independently predicting PFS. A nomogram constructed using the three parameters showed 49 months of PFS in patients with the best scores (0 or 1) and only 11 months in patients with a poor score (score 3). This study indicates that a positive FCH PET result in PC patients with biochemical recurrence predicts a shorter PFS and confirms the major impact of the FCH PET

  2. Prognostic value of metabolic parameters and clinical impact of 18F-fluorocholine PET/CT in biochemical recurrent prostate cancer

    International Nuclear Information System (INIS)

    To evaluate the therapeutic impact of 18F-fluorocholine (FCH) PET/CT in biochemical recurrent prostate cancer (PC) and to investigate the value of quantitative FCH PET/CT parameters in predicting progression-free survival (PFS). This retrospective study included 172 consecutive patients with PC who underwent FCH PET/CT for biochemical recurrence. Mean rising PSA was 10.7 ± 35.0 ng/ml. Patients with positive FCH PET were classified into three groups: those with uptake only in the prostatic bed, those with locoregional disease, and those with distant metastases. Referring physicians were asked to indicate the hypothetical therapeutic strategy with and without the FCH PET/CT results. Clinical variables and PET parameters including SUVmax, SUVpeak, SUVmean, total lesion choline kinase activity (TLCKA) and standardized added metabolic activity (SAM) were recorded and a multivariate analysis was performed to determine the factors independently predicting PFS. In 137 of the 172 patients, the FCH PET/CT scan was positive, and of these, 29.9 % (41/137) had prostatic recurrence, 42.3 % (58/137) had pelvic lymph node recurrence with or without prostatic recurrence, and 27.7 % (38/137) had distant metastases. The FCH PET/CT result led to a change in treatment plan in 43.6 % (75/172) of the 172 patients. Treatment was changed in 49.6 % (68/137) of those with a positive FCH PET/CT scan and in 20 % (7/35) of those with a negative FCH PET/CT scan. After a median follow-up of 29.3 months (95 % CI 18.9 - 45.9 months), according to multivariate analysis age <70 years, SAM ≥23 and SUVmean ≥3 were parameters independently predicting PFS. A nomogram constructed using the three parameters showed 49 months of PFS in patients with the best scores (0 or 1) and only 11 months in patients with a poor score (score 3). This study indicates that a positive FCH PET result in PC patients with biochemical recurrence predicts a shorter PFS and confirms the major impact of the FCH PET result on

  3. Linking hematological, biochemical, genotoxic, and behavioral responses to crude oil in the Amazon fish Colossoma macropomum (Cuvier, 1816).

    Science.gov (United States)

    Kochhann, Daiani; de Azevedo Brust, Sandra Maristher; Domingos, Fabíola Xochilt Valdez; Val, Adalberto Luis

    2013-08-01

    Despite safety protocols, crude oil extraction and transportation in the Amazon basin has a potential for inadvertent oil spills, which can impact aquatic organisms in local rivers. The objective of this study was to assess the effects of crude oil on juvenile Amazonian fish tambaqui, Colossoma macropomum, at various biological levels. Furthermore, the effect of crude oil on response to alarm substance, an important communication system in fish, was reported for the first time. Fish exposed to crude oil showed a 90 % decrease in their response to alarm substance and a 60 % decrease in swimming activity relative to control fish. Basic hematology was not affected, although an increase of 200 % of DNA damage and an increase of GST activity were observed in animals exposed to crude oil. Inverse correlations were found between genotoxicity end points and behavioral parameters, suggesting that genotoxic end points can also reflect behavioral changes. PMID:23539120

  4. Comparison of biochemical parameters of benzamide riboside, a new inhibitor of IMP dehydrogenase, with tiazofurin and selenazofurin.

    Science.gov (United States)

    Gharehbaghi, K; Sreenath, A; Hao, Z; Paull, K D; Szekeres, T; Cooney, D A; Krohn, K; Jayaram, H N

    1994-10-01

    The biochemical and cytotoxic activities of the IMP dehydrogenase (IMPDH) inhibitors benzamide riboside, tiazofurin, and selenazofurin were compared. These three C-nucleosides exert their cytotoxicity by forming an analogue of NAD, wherein nicotinamide is replaced by the C-nucleoside base. The antiproliferative activities of these three agents were compared in a panel of 60 human cancer cell lines. To examine the relationship of benzamide riboside and selenazofurin to tiazofurin, COMPARE computer analysis was performed, and correlation coefficients of 0.761 and 0.815 were obtained for benzamide riboside and selenazofurin, respectively. The biochemical activities of these agents were examined in human myelogenous leukemia K562 cells. Incubation of K562 cells for 4 hr with 10 microM each of benzamide riboside, selenazofurin and tiazofurin resulted in a 49, 71, and 26% decrease in IMPDH activity with a concurrent increase in intracellular IMP pools. As a consequence of IMPDH inhibition, GTP and dGTP concentrations were curtailed. These studies demonstrated that selenazofurin was the most potent of the three agents. To compare the cellular synthesis of NAD analogues of these agents, K562 cells were incubated with 10 microM each of benzamide riboside, tiazofurin and selenazofurin after prelabeling the cells with [2,8-3H]adenosine. The results demonstrated that benzamide riboside produced 2- and 3-fold more of NAD analogue (BAD) than tiazofurin and selenazofurin did. To elucidate the effects of the three compounds on other NAD-utilizing enzymes, the inhibitory activities of purified benzamide adenine dinucleotide (BAD), thiazole-4-carboxamide adenine dinucleotide (TAD) and selenazole-4-carboxamide adenine dinucleotide (SAD) were studied in commercially available purified preparations of lactate dehydrogenase, glutamate dehydrogenase and malate dehydrogenase. TAD and SAD did not inhibit these three dehydrogenases. Although BAD did not influence lactate and glutamate

  5. A study on biochemical facet of anemia in cancers: A strong link between erythropoietin and tumor necrosis factor alpha in anemic cancer patients

    Directory of Open Access Journals (Sweden)

    P Kalyani

    2015-01-01

    Full Text Available Background: The three direct factors that could lead cancer patients to anemia, apart from therapy are iron deficiency, inflammatory cytokines surge and decreased erythropoietin (Epo. Our aim was to quantify biochemical and hematologic markers serum Epo, ferritin (Fe and tumor necrosis factor-alpha (TNF-α along with hemoglobin (Hb to understand the associations between these factors, patient characteristics and anemia. Materials And Methods: The study group consisted of 100 anemic cancer patients and 80 controls. Biochemical marker levels were determined by the enzyme linked immunosorbent assay on an autoanalyser. Univarient analysis, t-test, ANOVA, Bonferroni test, linear regression was performed to find correlations and associations among various factors. Results: The baseline serum Epo (153.07 ± 173.88 vs. 23.607 ± 36.462 and Fe levels (233.53 ± 257.12 vs. 23.06 ± 20.04 were adequately high in cases compared with that controls (P ≤ 0.001. Considerable raise in TNF-α levels was also observed (16.26 ± 13.44 vs. 11.2375 ± 4.84 (P = 0.001. TNF-α correlated positively (P = 0.022 with Epo and Fe (P = 0.000, which was also evident from large effect size of Epo (r2 = 0.414, TNF-α (r2 = 0.369, Hb (r2 = 0.226. Epo and Hb were negatively correlated (β = −0.375, P = 0.001 and Epo production was found to be appropriate for the degree of anemia (O/P ratio of 3.51 ± 1.26 vs. 1.43 ± 0.47. A strong association was seen between Hb, Epo and TNF-α in hematological and gynecological malignancies for different grades of anemia. Men were more prone to life-threatening anemia (13% than women (9%. Conclusion: Anemia in cancers was not because of inadequate Epo or Fe levels, but because of improper Epo response. Further studies on molecular analysis of Epo, biochemical and molecular interplay between Epo and TNF-α could explain a rationale for anemia in cancers.

  6. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase

    Directory of Open Access Journals (Sweden)

    Flisser Ana

    2011-05-01

    Full Text Available Abstract Background Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP and the active labor phase (ActLP. Methods Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Results Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Conclusion Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  7. Diagnostic Value of Serum Lactate Dehydrogenase Isoenzyme and Amino Acid Patterns in Several Schistosomal and Non-Schistosomal Disorders as Compared to other Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    Samia A. Ahmed

    1996-01-01

    Full Text Available Serum lactate dehydrogenase (LDH isoenzyme and amino acid (a. a patterns were evaluated in comparison to several other biochemical parameters for liver and renal function with the objective of clarifying the differential diagnosis of hepatic disorders and predicting the outcome of schistosomal infection in Egyptian patients. Patients examined included those with complicated hepatic disorders and others with different stages of schistosomal infestation, hepatoma or bladder cancer, in addition to a normal control group. Several biochemical parameters appeared to be useful in establishing consistent differences or similarities between the studied groups. Examples are; elevated serum AST/ AL T ratio and methionine content in chronic schistosomiasis, elevated serum urea/creatinine ratio and leucine content in all schistosomal patients and extremely high levels of N-acetyl-β-D-glucosaminidase (NAG in the urine of non-schistosomal bladder cancer patients. In addition, characteristic LDH isoenzyme profiles distinguish between the studied groups, in particular separating chronic schistosomiasis from schistosomal bladder cancer and hepatoma from other hepatic disorders.

  8. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia. PMID:25758848

  9. The phytoconstituents and the comparative effects of aqueous extract of Irvingia gabonensis seeds and proviron on the biochemical parameters of male guinea pigs

    Institute of Scientific and Technical Information of China (English)

    Obianime AW; Uche FI

    2010-01-01

    Objective:To investigate the phytochemical screening and the effects of the aqueous extracts of the seeds ofIrvingia gabonensis on the biochemical parameters of male guinea pigs.Methods: The biochemical parameters were assayed using Randox Diagnostic kits, Phenolphthalein method and colorimetric method. The phytochemical screening was carried out using standard procedures.Results: Phytochemical investigations revealed the presence of flavonoids, tannins, carbohydrate, alkaloids, terpenoids, steroids, volatile oils, saponins and cardiac glycosides. The aqueous extract ofIrvingia gabonensis seeds (50 - 400 mg/kg) caused a statistically significant (P< 0.05 ANOVA) decrease in the levels of total cholesterol, urea, uric acid, total protein, prostatic, alkaline, and acid phosphatases. The highest reduction effect was obtained with uric acid at 400 mg/kg ofIrvingia gabonensis extract while the least effect was observed in total cholesterol. These effects were dose- and time- dependent.Conclusions: This shows that the seeds of Irvingia gabonensis have hepatoprotective, nephroprotective and cardio protective properties. The study therefore, supports the claims on the use of the seeds of this plant by traditional medicine practitioners as a hepatoprotective and nephroprotective agent. Although further studies need to be done to isolate, identify and characterize the active principles in the seeds of this plant.

  10. Roach (Rutilus rutilus) reproductive cycle: a study of biochemical and histological parameters in a low contaminated site.

    Science.gov (United States)

    Geraudie, Perrine; Gerbron, Marie; Hill, Elisabeth; Minier, Christophe

    2010-09-01

    Fish reproduction is subjected to worrying trends in many aquatic environments. In this study, we report the absence of histological and biochemical alterations in fish sampled in a low contaminated site (characterised by the absence of detectable oestrogenic activity and mutagenicity in sediment extracts). A total of 474 roach (Rutilus rutilus) were monthly sampled during 18 months, and no intersex fish were recorded after careful histological examination, thus indicating that the incidence of this phenomenon may be very low under natural conditions. Furthermore, mean male plasma vitellogenin concentration was 24 ng ml(-1) and was only slightly elevated during the spawning period (up to 120 ng ml(-1)) indicating that these low values may be characteristic of a low contaminated site. Of the male roach, 45.3% were sampled, a sex-ratio that did not significantly deviated from the expected 1:1 ratio between male and female. Results also showed that natural conditions can greatly affect the reproductive cycle of roach. Gametogenesis showed a biphasic pattern with first gonad maturation between September and December and a final maturation occurring at the end of winter/early spring. Under decreasing temperatures, particularly below 6 degrees C, gametogenesis was stopped or even regressed with secondary oocytes becoming rare under histological observation. Conversely, elevated temperatures during the winter lead to an earlier gonad maturation. PMID:19680761

  11. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata and Wheat (Triticum aestivum Seedlings

    Directory of Open Access Journals (Sweden)

    Mobin Afzal

    2012-03-01

    Full Text Available Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bean (Vigna radiata and Wheat (Triticum aestivum seedlings. The effect of cell phone Electromagnetic Radiations (EMR were determined by measuring membrane damage in terms of melondialdehyde [MDA] contents and changes in the levels of antioxidant enzymes like Guaiacol Peroxidase (GPX, Ascorbate Peroxidase (APX, and Catalase (CAT. Our results showed that cell phone EMR caused significant reduction in growth, fresh weight, dry weight, and relative water contents. Where as MDA contents and antioxidant enzymes were increased in stressed seedlings as compared to unstressed seedlings. We concluded that radiations emitted by mobile phone can induce oxidative stress which results in reduced growth and increase in the activity of antioxidant enzymes in mung bean and wheat seedlings.

  12. Changes in Some Biochemical Parameters and Somatic Cell Counts in the Milk of Buffalo and Cattle Suffering from Mastitis

    Directory of Open Access Journals (Sweden)

    Riaz Hussain§, Muhammad Tariq Javed and Ahrar Khan*

    2012-06-01

    Full Text Available The study was conducted on a total of 592 buffaloes and 453 cattle in their different stages of lactation to investigate the biochemical changes occurring in milk due to mastitis. California Mastitis Test (CMT was used to diagnose the mammary gland infection. The results revealed significant (P<0.0001 increase in pH, electrical conductivity, malondialdehyde and total dissolved solids, while decrease in fat, protein, lactose and solids not fat in milk samples of both mastitic buffaloes and cattle. The total somatic cell and neutrophil counts were significantly higher, while the macrophage and lymphocytes were lower in the milk of mastitic animals. The enzymes including lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase along with sodium were significantly higher in mastitic than healthy buffaloes. It was similar in cattle as well, with the exception of aspartate aminotransferase which was non-significant in cattle. The values of potassium, phosphorous, calcium, magnesium, zinc and iron were significantly higher in the milk of mastitic animals. The copper levels were significantly (P<0.0001 lower in mastitic than in healthy buffaloes, while it showed non-significant difference in cattle. The investigation of enzymes, lipid peroxidation product and milk electrical conductivity in present study appeared suitable diagnostic tools for identification of mastitis.

  13. Mutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents; A comprehensive survey of genetic and biochemical characteristics

    Energy Technology Data Exchange (ETDEWEB)

    Collins, A.R. (Aberdeen University (United Kingdom). Department of Molecular and Cell Biology)

    1993-01-01

    Mutant rodent cell lines with hypersensitivity to DNA damage resulting from a defect in cellular response to the damage have contributed to many recent advances in our knowledge of DNA-repair processes. Many of these mutants have been classified by genetic complementation analysis. They proved excellent recipients of human DNA in transfection experiments, and from those transfectants with restored resistance to DNA damage it has been possible to isolate the foreign DNA responsible for correcting the defect. Several human genes coding for DNA-repair proteins have now been cloned. Until recently, there was apparently no correlation at the genetic level between artificially produced rodent mutants and the inherited human diseases associated with sensitivity to DNA-damaging agents, but several of the human genes cloned in rodent mutants now turn out to correct defects in cells representing the human diseases, too. Others are homologous to yeast DNA-repair genes, and it is clear that at least some of the proteins involved in DNA-repair are highly conserved through evolution. Mutants have provided material for comparative biochemical studies of DNA repair too, and we are nearer to understanding the complexities of this process. This article presents tables that list rodent mutant cell lines sensitive to ultraviolet light, to ionizing radiation and to cross-linking agents. (author). 126 refs., 3 tabs.

  14. The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.

    Science.gov (United States)

    Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

    1991-02-01

    Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis. PMID:2019018

  15. Effects of supplementation of threshed sorghum top with selected browse plant foliage on haematology and serum biochemical parameters of Red Sokoto goats.

    Science.gov (United States)

    Okunade, Sunday Adewale; Isah, Olubukola Ajike; Oyekunle, Mufutau Atanda; Olafadehan, Olurotimi Ayobami; Makinde, Olayinka John

    2016-06-01

    The haematological and biochemical parameters of 24 growing Red Sokoto bucks (9.00 ± 0.25 kg body weight) fed threshed sorghum top (TST) with or without five different browse plant foliage Afzelia africana (AA), Daniellia oliveri (DO), Piliostigma thonningii (PT), Pterocarpus erinaceus (PE) and Annona senegalensis (AS) supplements were studied using a completely randomized design. All haematological parameters were (P < 0.05) lower in TST-fed goats compared with TST-supplemented goats, except for mean corpuscular haemoglobin concentration (MCHC), lymphocytes and monocytes which did not follow a particular pattern. Packed cell volume, haemoglobin and monocytes were higher for AA-supplemented goats while MCHC was reduced relative to other supplements (P < 0.05). White blood cell counts were increased in DO lambs compared to other supplements (P < 0.05). Serum proteins, creatinine, alanine transaminase and alkaline phosphate were reduced in TST compared with the supplements (P < 0.05). Cholesterol was higher for PT and AA than other diets (P < 0.05). Aspartate transaminase was reduced in TST goats relative to the supplements (P < 0.05). Except for potassium which was reduced in AS (P < 0.05), all serum major minerals were similar among diets. Results indicate that the entire browse fodder are good supplements to low-quality TST, though A. africana appears to have a better supplementary effect on haematological and biochemical parameters of the goats. PMID:27010715

  16. Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+.

    Science.gov (United States)

    Ates, Burhan; Orun, Ibrahim; Talas, Zeliha Selamoglu; Durmaz, Gokhan; Yilmaz, Ismet

    2008-03-01

    This study was carried out to understand the preventive effect of selenium (Se4+) on heavy metal stress induced by lead and copper in rainbow trout (Oncorhynchus mykiss). Variation in glutathione peroxidase (Se-GSH-Px) and superoxide dismutase (SOD) activity, and in malondialdehyde (MDA) levels in liver, spleen, heart, and brain tissues of rainbow trout after 72 h of exposure to Pb2+ and Cu2+ were investigated in the presence and absence of Se4+. In the presence of Se4+, Se-GSH-Px activity and SOD activity were found to be higher and MDA levels were lower compared with in its absence. Hematological parameters were also determined and it has been observed that total leukocyte count (WBC), mean cell volume (MCV), and mean cell hemoglobin (MCH) were increased and erythrocyte number (RBC), hemoglobin (Hb), and hematocrit value (Hct; P heavy metals in the absence of selenium. Selenium presence recovered hematological parameters to normal levels. In the light of our findings, it could be stated that Pb2+ and Cu2+ lead to dramatic changes in biochemical and hematological parameters and selenium caused these parameters to converge to control levels when it was administered concurrently with these heavy metals. PMID:18649023

  17. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    Science.gov (United States)

    Zhou, Zhenming; Zhou, Bo; Ren, Liping; Meng, Qingxiang

    2014-01-01

    Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg) were used to compare a standard total mix ration (TMR) with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML) or sun-dried mulberry fruit pomace (SMFP). Experimental diets had similar amounts of crude protein (CP), acid detergent fiber (ADF), and metabolizable energy (ME). Animals were divided into three groups: control group (CONT), 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG), and dry matter intake (DMI), was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks). There were no differences in final body weight (P = 0.743), ADG (P = 0.425), DMI (P = 0.642), or ADG/DMI (P = 0.236) between the groups. There were no differences (P = 0.2024) in rumen pH values; ammonia N was lower (P = 0.0076) in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098). No differences were observed in carcass characteristics (P ≥ 0.513), tenderness (P = 0.844), adipose and lean color values (P ≥ 0.149), and chemical composition (P ≥ 0.400); however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034). In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content. PMID:24427304

  18. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    Directory of Open Access Journals (Sweden)

    Zhenming Zhou

    Full Text Available Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg were used to compare a standard total mix ration (TMR with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML or sun-dried mulberry fruit pomace (SMFP. Experimental diets had similar amounts of crude protein (CP, acid detergent fiber (ADF, and metabolizable energy (ME. Animals were divided into three groups: control group (CONT, 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG, and dry matter intake (DMI, was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks. There were no differences in final body weight (P = 0.743, ADG (P = 0.425, DMI (P = 0.642, or ADG/DMI (P = 0.236 between the groups. There were no differences (P = 0.2024 in rumen pH values; ammonia N was lower (P = 0.0076 in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098. No differences were observed in carcass characteristics (P ≥ 0.513, tenderness (P = 0.844, adipose and lean color values (P ≥ 0.149, and chemical composition (P ≥ 0.400; however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034. In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content.

  19. Preparation for high altitude expedition and changes in cardiopulmonary and biochemical laboratory parameters with ascent to high altitude in transplant patients and live donors.

    Science.gov (United States)

    Suh, Kyung-Suk; Kim, Taehoon; Yi, Nam-Joon; Hong, Geun

    2015-11-01

    High-altitude climbing has many risks, and transplant recipients should discuss the associated risks and means of preparation with their physicians. This study aimed to help prepare athletic transplant donors and recipients for mountain climbing and was designed to evaluate physical performance and changes in cardiopulmonary and biochemical laboratory parameters of transplant recipients and donors in extreme conditions of high altitude. Ten subjects-six liver transplant recipients, two liver donors, and one kidney transplant recipient and his donor-were selected for this expedition to Island Peak, Himalayas, Nepal. Six healthy subjects joined the group for comparison. Blood samplings, vital signs, and oxygen saturation were evaluated, as was the Lake Louise acute mountain sickness score. All transplant subjects and donors reached the base camp (5150 m), and two liver transplant recipients and a liver donor reached the summit (6189 m). The blood levels of immunosuppressants were well maintained. The serum erythropoietin level was significantly higher in transplant recipients taking tacrolimus. With proper preparation, certain liver transplant patients and donors can tolerate strenuous physical activity and can tolerate high altitude similarly to normal healthy control subjects without significant biochemical laboratory changes in liver and renal function. PMID:26331794

  20. Effect of the Telephone-Delivered Nutrition Education on Dietary Intake and Biochemical Parameters in Subjects with Metabolic Syndrome

    OpenAIRE

    Kim, Juyoung; Bea, Wookyung; Lee, Kiheon; Han, Jongsoo; Kim, Sohye; Kim, Misung; Na, Woori; Sohn, Cheongmin

    2013-01-01

    As prevalence of metabolic syndrome has rapidly increased over the past decade, lifestyle changes including dietary habits are considered as a therapeutic cornerstone for metabolic syndrome, cardiovascular complications and type 2 diabetes. We evaluated the effectiveness of a telephone-delivered nutrition education to improve metabolic parameters compared with a single-visit with a dietitian in subjects with metabolic syndrome. A total of seventy-one adults who met diagnostic criteria for the...

  1. Evaluation of changes in blood hematological and biochemical parameters in response to Islamic fasting and regular physical activity in male and female subjects

    Directory of Open Access Journals (Sweden)

    Seyyed Reza Attarzadeh Hosseini

    2015-09-01

    Full Text Available Introduction: Fasting during Ramadan is a religious obligation for healthy adult Muslims. This study aimed to investigate the effects of Ramadan fasting and physical activity on blood hematological and biochemical parameters in male and female subjects. Methods: In this study, 50 healthy subjects were randomly assigned to four groups: male control fasting (MCF group (n=13, male training fasting (MTF group (n=13, female control fasting (FCF group (n=12, and female training fasting (FTF group (n=12, and were compared in two stages (before and after the intervention. For inter- and intra-group comparisons, a repeated measure ANOVA was applied. Results: The results showed a significant decline in body weight, body mass index, body fat percentage, and waist-to-hip ratio after Ramadan fasting, compared to the pre-Ramadan period. Also, significant changes were observed in red blood cell count and hematocrit level (P

  2. [Effect of dietotherapy with food-stuffs for enteral nutrition on the dynamic of clinical and biochemic parameters in biliary tract diseases].

    Science.gov (United States)

    Plotnikova, O A; Meshcheriakova, V A; Sharfetdinov, Kh Kh; Petrovskaia, O B; Mokhova, E O

    2005-01-01

    It was investigated the influence of dietary therapy with enteral formula "Nutricomp AND Braum fiber" on clinical and biochemic parameters in biliary tract diseases. Also it was carried out the comparative estimation of the gallbladder motility in this contingent of patients in process of the standard food loading and the loading with the tested enteral formula (25 g carbohydrates). It was indicated that the including of enteral formula "Nutricomp AND Braum fiber" (209 g/day) in traditional hypocaloric diet promotes the decrease of cholesterol level and activity of alkaline phosphatase. The gallbladder motility was some smaller after the consuming of enteral formula "Nutricomp AND Braum Diabetes" compared with the standard food loading (25 g carbohydrates). PMID:16313133

  3. Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and Leporinus obtusidens (Valenciennes, 1837

    Directory of Open Access Journals (Sweden)

    Vania Lucia Loro

    2015-03-01

    Full Text Available Rhamdia quelen (silver catfish and Leporinus obtusidens (piava were exposed to a commercial formulation Roundup(r, a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT and aspartate aminotransferase (AST activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase activities. Plasmatic glucose levels were reduced in both species, whereas the transaminase activities (ALT and AST increased after exposure to the herbicide. Herbicide exposure increased protein and glucose levels in the mucus layer in both species. There was a reduction in both NTPDase and ecto-5'-nucleotidase activity in the brain of piava, and increased enzyme activity in silver catfish at both concentrations tested. The species showed an increase in protein carbonyl in the liver after exposure to both concentrations of the glyphosate. Our results demonstrated that exposure to Roundup(r caused liver damage, as evidenced by increased plasma transaminases and liver protein carbonyl in both of the fish species studied. The mucus composition changed and hypoglycemia was detected after Roundup(r exposure in both species. Brain nucleotide hydrolysis showed a different response for each fish species studied. These parameters indicated some important and potential indicators of glyphosate contamination in aquatic ecosystems.

  4. Biochemical, photosynthetic and productive parameters of Chinese cabbage grown under blue-red LED assembly designed for space agriculture

    Science.gov (United States)

    Avercheva, Olga; Berkovich, Yuliy A.; Smolyanina, Svetlana; Bassarskaya, Elizaveta; Pogosyan, Sergey; Ptushenko, Vasiliy; Erokhin, Alexei; Zhigalova, Tatiana

    2014-06-01

    Currently light emitting diodes (LEDs) are considered to be most preferable source for space plant growth facilities. We performed a complex study of growth and photosynthesis in Chinese cabbage plants (Brassica chinensis L.) grown with continuous LED lighting based on red (650 nm) and blue (470 nm) LEDs with a red to blue photon ratio of 7:1. Plants grown with high-pressure sodium (HPS) lamps were used as a control. PPF levels used were about 100 μmol/(m2 s) (PPF 100) and nearly 400 μmol/(m2 s) (PPF 400). One group of plants was grown with PPF 100 and transferred to PPF 400 at the age of 12 days. Plants were studied at the age of 15 and 28 days (harvest age); some plants were left to naturally end their life cycle. We studied a number of parameters reflecting different stages of photosynthesis: photosynthetic pigment content; chlorophyll fluorescence parameters (photosystem II quantum yield, photochemical and non-photochemical chlorophyll fluorescence quenching); electron transport rate, proton gradient on thylakoid membranes (ΔpH), and photophosphorylation rate in isolated chloroplasts. We also tested parameters reflecting plant growth and productivity: shoot and root fresh and dry weight, sugar content and ascorbic acid content in shoots. Our results had shown that at PPF 100, plants grown with LEDs did not differ from control plants in shoot fresh weight, but showed substantial differences in photophosphorylation rate and sugar content. Differences observed in plants grown with PPF 100 become more pronounced in plants grown with PPF 400. Most parameters characterizing the plant photosynthetic performance, such as photosynthetic pigment content, electron transport rate, and ΔpH did not react strongly to light spectrum. Photophosphorylation rate differed strongly in plants grown with different spectrum and PPF level, but did not always reflect final plant yield. Results of the present work suggest that narrow-band LED lighting caused changes in Chinese

  5. Methods of mesophyll conductance estimation: its impact on key biochemical parameters and photosynthetic limitations in phosphorus-stressed soybean across CO2.

    Science.gov (United States)

    Singh, Shardendu K; Reddy, Vangimalla R

    2016-06-01

    Despite the development of various methods, the rapid estimation of mesophyll conductance (gm ) for a large number of samples is still a daunting challenge. Although the accurate estimation of gm is critical to partition photosynthetic limitations by stomatal (Ls ) and mesophyll (Lm ) conductance and by photo-biochemical (Lb ) processes, the impact of various gm estimation methods on this is ambiguous. As phosphorus (P) starvation and elevated CO2 (eCO2 ) strongly affect photosynthetic processes, their combined effect on the proportional changes in these limitations are not well understood. To investigate this, while also evaluating distinct recent methods of gm estimation sharing few common theories and assumptions, soybean was grown under a range of P nutrition at ambient and eCO2 . Methods significantly affected gm and carboxylation efficiency (VCmax ) but not other photosynthetic parameters. In all the methods, all photosynthetic parameters responded similarly to treatments. However, the percentage difference between VCmax assuming finite and infinite gm was highly inconsistent among methods. The primary mechanism responsible for P limitation to soybean photosynthesis was not CO2 diffusion limitations but Lb comprised of reduced chlorophyll, photochemistry and biochemical processes. The eCO2 decreased Lb but increased Lm without affecting Ls across leaf P concentration. Although each method explored advances of our understanding about gm variability, they all require assumptions of varying degrees, which lead to the discrepancy in the gm values. Among the methods, the oxygen sensitivity-based gm estimation appeared to be suitable for the quick assessment of a large number of samples or genotypes. Digital tools are provided for the easy estimation of gm for some methods. PMID:26806194

  6. Salivary biochemical markers as potential acute toxicity parameters for acute radiation injury: A study on small experimental animals.

    Science.gov (United States)

    Soni, S; Agrawal, P; Kumar, N; Mittal, G; Nishad, D K; Chaudhury, N K; Bhatnagar, A; Basu, M; Chhillar, N

    2016-03-01

    Researchers have been evaluating several biodosimetric/screening approaches to assess acute radiation injury, related to mass causality. Keeping in mind this background, we hypothesized that effect of whole-body irradiation in single fraction in graded doses can affect the secretion of various salivary components that could be used as acute radiation injury/toxicity marker, which can be used in screening of large population at the time of nuclear accidents/disaster. Thirty Sprague Dawley rats treated with whole-body cobalt-60 gamma irradiation of dose 1-5 Gy (dose rate: 0.95 Gy/min) were included in this study. Whole mixed saliva was collected from all animals before and after radiation up to 72 h postradiation. Saliva was analyzed for electrolytes, total protein, urea, and amylase. Intragroup comparison of salivary parameters at different radiation doses showed significant differences. Potassium was significantly increased as the dose increased from 1 Gy to 5 Gy (p 0.5). Sodium was significantly altered after 3-5 Gy (p 0.5), except 1 and 2 Gy, whereas changes in sodium level were nonsignificant (p > 0.5). Urea, total protein, and amylase levels were also significantly increased as the radiation dose increased (p 0.5). This study suggests that salivary parameters were sensitive toward radiation even at low radiation dose which can be used as a predictor of radiation injury. PMID:25813962

  7. INFLUENCE OF SURFACE-ACTIVE RHAMNOLIPID BIOCOMPLEX AND ETHYLTHIOSULFANILATE ON GROWTH AND BIOCHEMICAL PARAMETERS OF PLANTS IN THE OIL CONTAMINATED SOIL

    Directory of Open Access Journals (Sweden)

    A. R. Banya

    2015-10-01

    Full Text Available The aim of the work was to study the influence of rhamnolipid biocomplex and ethylthiosulfanilate on field pea and sorghum plants when growing in petroleum contaminated soil. Plant seeds were treated with solutions of rhamnolipid biocomplex or ethylthiosulfanilate (0.01 g/l before planting and grown in containers with soil artificially contaminated with petroleum (5, 8 and 10%. Effect of rhamnolipid biocomplex and ethylthiosulfanilate was determined by the determination of growth (weight, length plants and biochemical parameters (content of photosynthetic pigments, hydrogen peroxide, malondialdehyde. The stimulating effect of rhamnolipid biocomplex and ethylthiosulfanilate on growth parameters of field pea was shown: the shoot mass has significantly increased on 39%, root mass – on 26% if compared with the control. For sorghum somewhat smaller increase of growth parameters was observed. Under the influence of rhamnolipid biocomplex and ethylthiosulfanilate the content of photosynthetic pigments in field pea and sorghum has also increased. It was determined that the action rhamnolipid biocomplex and ethylthiosulfanilate promoted the decrease of indicators of plant oxidative reactions if compared with control: the content of hydrogen peroxide – in average on 15% and 16%, malondialdehyde – on 13.5% and 16% respectively. The results of the study testify to the effectiveness of rhamnolipid biocomplex and ethylthiosulfanilate as growth stimulators for field pea and sorghum, as well as improvement of adaptive capability of plants to unfavorable conditions. It creates the prospects of their application as effective and ecologically safe substances for the intensification of contaminated soil phytoremediation.

  8. High sensitive C-reactive protein (hs-CRP level and biochemical parameters for prehypertension and prediabetes diagnosis

    Directory of Open Access Journals (Sweden)

    Yuttana Sudjaroen

    2015-01-01

    Full Text Available Context: Coronary atherosclerosis still presents one of the main causes of death. Efficacious prevention should focus on the early control of cardiovascular risk factors, including lipid profiles, which are unable early detect in subclinical cases. High-sensitive C-reactive protein (hs-CRP can prove to be an early cardiac risk predictor. Aims: 1 To compare hs-CRP levels between healthy volunteer with normal blood pressure and those with prehypertension, and 2 to use hs-CRP levels along with other risks to be a cardiac risk predictor. Setting and Design: This was a cross-sectional study for 6 months′ duration from January to June 2013 at Kudjab Hospital located in Udon Thani province, Thailand. Materials and Methods: Forty (40 healthy volunteers with prehypertension and 40 volunteers with normal blood pressure were included in the study. Both groups were similar in age range and sex. Twelve-hour (12-h fasting blood samples were collected from all the participants. Serum was assayed for hs-CRP and lipid profile. Results: All of parameters were statistically significant difference (P < 000.1. The hs-CRP level (6.27 ± 7.8 mg/L was elevated in the prehypertension group. The relative risk of hs-CRP for prehypertension was 6.3 with the odds ratio of 15.48, whereas the relative risk of lipid profiles for prehypertension prediction was only 1.28, with the odds ratio of 1.67. Statistical Analysis: SPSS version 11.0 using the unpaired t-test for comparing demographic data and blood parameters and risk prediction of hs-CRP and lipid profiles were calculated by relative risk with odds ratio [95% confidence interval (CI]. Conclusions: Hs-CRP is an early cardiac risk predictor even with normal lipid profile, and can help measure additional risk especially subclinical people such as prehypertension.

  9. The Effect of the Adsorbent Primix-Alfasorb on the Morphological and Biochemical Blood Parameters of Breeding Gilts

    Directory of Open Access Journals (Sweden)

    Larisa Caisîn

    2012-05-01

    Full Text Available The studies were conducted at the state enterprise “Moldsuinhibrid” (Republic Moldova in 2011 using piglets of Landrace breed. The animals selected for the experiment on the basis of analogues (including origin, age, body weight and health status were divided into four groups of 10 animals each. The basic diet for the piglets in the experimental groups was supplemented with the adsorbent Primix-Alfasorb at the level of 0.2, 0.4 and 0.6 kg per ton of mixed fodder.It was established that the addition of the adsorbent Primix-Alfasorb in the mixed fodder had contributed to the increase in body weight in different age periods; to the end of the trial the live weight of the piglets in groups EG1, EG2 and EG3 was higher by 8.72, 5.83, and 7.93 % compared with the control group.The analysis of the blood of the experimental animals the diet of which was supplemented with the adsorbent Primix-Alfasorb, showed that all parameters were within the physiological norm, and the increase in the amount of total protein indicated a strengthening of the metabolic processes in the animals. It was determined that the optimum level of the addition of the adsorbent Primix-Alfasorb in the mixed fodder for piglets was of 0.2 kg/t.

  10. Zearalenone-induced changes in biochemical parameters, oxidative stress and apoptosis in cardiac tissue: Protective role of crocin.

    Science.gov (United States)

    Salem, I Ben; Boussabbeh, M; Neffati, F; Najjar, M F; Abid-Essefi, S; Bacha, H

    2016-06-01

    Zearalenone (ZEN) is a mycotoxin from Fusarium species commonly found in food commodities and is known to cause reproductive disorders. Several in vivo studies have shown that ZEN is haematotoxic and hepatotoxic and causes several alterations of immunological parameters. Meantime, the available information on the cardiotoxic effects of ZEN is very much limited. In the present study, we investigated the toxic effects of ZEN in heart tissues of Balb/c mice. We demonstrated that ZEN (40 mg kg(-1) body weight (b.w.)) increased creatine phosphokinase, lactate dehydrogenase, aspartate transaminase, alanine transaminase, total cholesterol and triglyceride levels and induced oxidative stress as monitored by measuring the malondialdehyde level, the generation of protein carbonyls, the catalase and superoxide dismutase activity and the expression of the heat shock proteins (Hsp 70). We also demonstrated that acute administration of ZEN triggers apoptosis in cardiac tissue. Furthermore, we aimed to evaluate the safety and efficacy of crocin (CRO), a natural carotenoid, to prevent ZEN-induced cardiotoxicity in mice. In fact, combined treatment of ZEN with different doses of CRO (50, 100, and 250 mg kg(-1) b.w.) showed a significant reduction of ZEN-induced toxicity for all tested markers in a dose-dependent manner. It could be concluded that CRO was effective in the protection against ZEN-induced toxicity in cardiac tissue. PMID:26231423

  11. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Al-Okbi, S. Y.; Mohamed, D. A.; Donya, S. M.; Abd El Khalek, A. B.

    2011-07-01

    The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum) were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, {beta}-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory bio markers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures. (Author) 61 refs.

  12. Aluminium-induced changes in hemato-biochemical parameters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid.

    Science.gov (United States)

    Yousef, Mokhtar I

    2004-06-01

    For a long time, aluminium (Al) has been considered an indifferent element from a toxicological point of view. In recent years, however, Al has been implicated in the pathogenesis of several clinical disorders, such as dialysis dementia, the fulminant neurological disorder that can develop in patients on renal dialysis. Therefore, the present experiment was carried out to determine the effectiveness of l-ascorbic acid (AA) in alleviating the toxicity of aluminium chloride (AlCl3) on certain hemato-biochemical parameters, lipid peroxidation and enzyme activities of male New Zealand white rabbits. Six rabbits per group were assigned to 1 of 4 treatment groups: 0mg AA and 0mg AlCl3/kg body weight (BW) (control); 40 mg AA/kg BW; 34 mg AlCl3/kg BW (1/25 LD50); 34 mg AlCl3 plus 40 mg AA/kg BW. Rabbits were orally administered their respective doses every other day for 16 weeks. Evaluations were made for lipid peroxidation, enzyme activities and hemato-biochemical parameters. Results obtained showed that AlCl3 significantly (PGST) and the levels of sulfhydryl groups (SH groups) in rabbit plasma, liver, brain, testes and kidney. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AlP), acid phosphatase (AcP), and phosphorylase activities were significantly decreased in liver and testes due to AlCl3 administration. While, plasma, liver, testes and brain lactate dehydrogenase (LDH) activities were significantly increased. Contrariwise, the activity of acetylcholinesterase (AChE) was significantly decreased in brain and plasma. Aluminium treatment caused a significant decrease in plasma total lipids (TL), blood haemoglobin (Hb), total erythrocytic count (TEC) and packed cell volume (PCV), and increased total leukocyte count (TLC) and the concentrations of glucose, urea, creatinine, bilirubin and cholesterol. Ascorbic acid alone significantly decreased the levels of free radicals, TL, cholesterol, glucose and creatinine, and increased the

  13. EFFECT OF FEEDING AMARANTH (AMARANTHUS ON AQUACULTURAL AND SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF AGE-2 CARP

    Directory of Open Access Journals (Sweden)

    R. Palamarchuk

    2016-06-01

    with artificial feeds of improved composition. This will allow reducing the cost of artificial feeds, obtaining higher growth rate, and improving physiological parameters of cultured carp.

  14. Relationship of tumor necrosis factor alpha genotypes with various biochemical parameters of normal, over weight and obese human subjects

    International Nuclear Information System (INIS)

    Tumor Necrosis Factor (TNF-alpha) is expressed primarily in adipocytes and elevated levels of this cytokine have been associated with obesity. The purpose of this investigation was to test whether the TNF-alpha -308 polymorphism were associated with insulin resistance or obesity related traits in non-diabetic and diabetic patients visiting Sheikh Zayed Hospital, Lahore, Fatima Hospital and Irfan Clinic in Sargodha. In non diabetic subjects the AA allele carriers, compared with homozygous G allele carriers had significantly lower (28%) triglyceride values and 15% higher HDL yal ues, whereas other parameters tested 81id not show any significant variation. In diabetic patients the AA allele carriers, compared with GG allele carriers, besides having 31 % higher FBS and 26% higher creatinine, had 20% higher cholesterol and 34% higher triglycerides. The HDL values were 14% less, compared to GG allele carriers. In normal subjects (BMI 22.85:1:0.25 kgim2), the AA allele carriers showed 132%, 125%, 65% and 112% higher triglycerides, cholesterol and LDL values compared with GG allele carriers. The HDL and creatinine did not show any significant change. In the overweight subjects (BMI: 27.17+-0.17 kgim/sup 2/) all these values were lower than in AA allele carriers compared with GG allele carriers. The AA allele carries had FBS, triglycerides, cholesterol and LDL 28%, 48%, 14% and 14% lower than in the GG allele' carriers, respectively. In obese subjects, (BMI: 36.73+-0.78kgm/sup 2/), however, the FBS, triglycerides, cholesterol and creatinine values were 5%, 8%, 7% and 14% higher in AA allele carries compared to GG allele carriers, respectively. The LDL content was 8% lower in AA allele carrier as compared with the respective GG allele carriers, It is concluded that replacement of G at -308 with A leads to reduced risk for cardiovascular disease in non-diabetic subject, whereas in diabetic patients this mutation-increases the risk of CVD. Using BMI as index of obesity, it was

  15. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

    Directory of Open Access Journals (Sweden)

    Donya, S. M.

    2011-09-01

    Full Text Available The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, β-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory biomarkers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures.El objetivo de la presente investigación fue encontrar extractos de alimentos vegetales y probióticos que puedan tener bioactividad hacía la inflamación crónica. Mezclas de tres extractos de alimentos vegetales conocidos por su riqueza en compuestos fenólicos, carotenoides y tocoferoles han sido preparadas. La actividad anti-inflamatoria de las diferentes mezclas y de bacterias probióticas (Bifidobacterium bifidum fue evaluada en artritis adyuvante en ratas. El efecto anti-inflamatorio, mecanismo de acción y salubridad de las tres mezclas y de Bifidobacterium bifidum ha sido estudiado mediante la medida del tamaño de la inflamación y la determinación de biomarcadores de inflamación y estrés oxidativo, del perfil de bacterias del colón y de parámetros citogen

  16. Effect of Abouthiouline, a novel drug with therapeutic potential as Antithyroid, on some biochemical and hematologic parameters in mice and rats

    International Nuclear Information System (INIS)

    Abouthiouline (1-Cyclohexyl-3 (3-quinolyl)-2-thiourea) is a novel compound with antithyroid activity. Abouthiouline (ABL) was designed based on structure-activity relationships (E-state indexes) aimed at reducing the antioxidant properties of the compound by modification of acyclic thiourylene moiety. Antioxidant effects of currently available treatments such as propylthiouracil (PTU), methimazole (MTM) are associated with an incidence of agranalocytosis and aplastic anemia. In the present study, the preclinical toxicology of ABL was determined in mice and rats and compared with two reference compounds, namely, propylthiouracil, methimazole. Following short-term administration (7 days) to mice, ABL had minimal effects on biochemical parameters, although significant reductions in both total protein and albumin were noted. Long term studies although significant reductions in both total protein and albumin were noted. Long term studies (30 days) in rats revealed significant effects of Abouthiouline, propylthiouracil and methimazloe on serum electrolyte and glucose levels. Abouthiouline had no detrimental effects on hematologic parameters. However, total WBC count (propylthiouracil) and neutrophil levels (propylthiouracil and methimazole) were significantly decreased among other treatment groups. The results of this investigation suggest that Abouthiouline is a promising new antithyroid therapy with a reduced risk of hematologic toxicity that is associated with PTU and MTM. Further studies are warranted to assess the safety and efficacy of Abouthiouline. (author)

  17. Linking the Recurrence Time of Earthquakes to Source Parameters: A Dream or a Real Possibility?

    Science.gov (United States)

    Bizzarri, Andrea; Crupi, Paola

    2014-10-01

    By using a single-degree-of-freedom spring-slider analog fault model, we generate a synthetic catalog of nearly 500 different seismic sequences. We explore the parameter space by assuming different values of constitutive parameters and tectonic environment. We also consider three different versions of the rate-dependent and state-dependent friction laws [the Dieterich-Ruina (DR), the Ruina-Dieterich (RD) and the Chester-Higgs (CH) models], and different approximations of the behavior of the friction at high sliding speeds, as well as the radiation damping effects. Our results indicate that for all the considered models, the recurrence time ( T cycle) exhibits an inverse proportionality on the loading rate; a linear, positive dependence on the effective normal stress; and a linear, negative dependence on the characteristic distance controlling the state variable evolution. These results confirm and generalize previous studies. Remarkably, we found here that the coefficients of proportionality strongly depend on the adopted friction model, on the high speed behavior and on the reference set of parameters. Notably, we also found that the positive proportionality between T cycle and the difference b - a, confirmed for DR and RD laws, does not hold in general for the CH law. Overall, we conclude that even in the simplest (and idealized) case of characteristic earthquakes considered here, in which the limiting cycle is reached by the system, and even in the framework of a very simplified fault model, the possibility to a priori predict, through an universal analytical relation, the inter-event time of an impending earthquake still remains only a dream. On the other hand, a numerical prediction of T cycle would require the exact knowledge of the rheological model (and its parameters at all times over the entire life of the fault) and the actual state of the fault, which indeed are often unknown.

  18. Linking Particle and Pore-Size Distribution Parameters to Soil Gas Transport Properties

    DEFF Research Database (Denmark)

    Arthur, Emmanuel; Møldrup, Per; Schjønning, Per;

    2012-01-01

    topsoil of two Danish arable fields representing two natural clay gradients, Dp/Do and ka were measured at soil water matric potentials between −1 and −100 kPa on undisturbed soil cores. The Rosin–Rammler particle size distribution parameters α and β (characteristic particle size and degree of sorting......, respectively) and the Campbell water retention parameter b were used to characterize particle and pore size distributions, respectively. Campbell b yielded a wide interval (4.6–26.2) and was highly correlated with α, β, and volumetric clay content. Both Dp/Do and ka followed simple power-law functions (PLFs......) of air-filled porosity (εa). The PLF tortuosity–connectivity factors (X*) for Dp/Do and ka were both highly correlated with all basic soil characteristics, in the order of volumetric clay content = Campbell b > gravimetric clay content > α > β. The PLF water blockage factors (H) for Dp/Do and ka were...

  19. Acquisition of isotopic composition for surface snow in East Antarctica and the links to climatic parameters

    Science.gov (United States)

    Touzeau, Alexandra; Landais, Amaëlle; Stenni, Barbara; Uemura, Ryu; Fukui, Kotaro; Fujita, Shuji; Guilbaud, Sarah; Ekaykin, Alexey; Casado, Mathieu; Barkan, Eugeni; Luz, Boaz; Magand, Olivier; Teste, Grégory; Le Meur, Emmanuel; Baroni, Mélanie; Savarino, Joël; Bourgeois, Ilann; Risi, Camille

    2016-04-01

    The isotopic compositions of oxygen and hydrogen in ice cores are invaluable tools for the reconstruction of past climate variations. Used alone, they give insights into the variations of the local temperature, whereas taken together they can provide information on the climatic conditions at the point of origin of the moisture. However, recent analyses of snow from shallow pits indicate that the climatic signal can become erased in very low accumulation regions, due to local processes of snow reworking. The signal-to-noise ratio decreases and the climatic signal can then only be retrieved using stacks of several snow pits. Obviously, the signal is not completely lost at this stage, otherwise it would be impossible to extract valuable climate information from ice cores as has been done, for instance, for the last glaciation. To better understand how the climatic signal is passed from the precipitation to the snow, we present here results from varied snow samples from East Antarctica. First, we look at the relationship between isotopes and temperature from a geographical point of view, using results from three traverses across Antarctica, to see how the relationship is built up through the distillation process. We also take advantage of these measures to see how second-order parameters (d-excess and 17O-excess) are related to δ18O and how they are controlled. d-excess increases in the interior of the continent (i.e., when δ18O decreases), due to the distillation process, whereas 17O-excess decreases in remote areas, due to kinetic fractionation at low temperature. In both cases, these changes are associated with the loss of original information regarding the source. Then, we look at the same relationships in precipitation samples collected over 1 year at Dome C and Vostok, as well as in surface snow at Dome C. We note that the slope of the δ18O vs. temperature (T) relationship decreases in these samples compared to those from the traverses, and thus caution is

  20. Acquisition of isotopic composition for surface snow in East Antarctica and the links to climatic parameters

    Directory of Open Access Journals (Sweden)

    A. Touzeau

    2015-11-01

    Full Text Available The isotopic composition of oxygen and hydrogen in ice cores are invaluable tools for the reconstruction of past climate variations. Used alone, they give insights into the variations of the local temperature, whereas taken together they can provide information on the climatic conditions at the point of origin of the moisture. However, recent analyses of snow from shallow pits indicate that the climatic signal can become erased in very low accumulation regions, due to local processes of snow reworking. The signal to noise ratio decreases and the climatic signal can then only be retrieved using stacks of several snow pits. Obviously, the signal is not completely lost at this stage, otherwise it would be impossible to extract valuable climate information from ice cores as has been done, for instance, for the last glaciation. To better understand how the climatic signal is passed from the precipitation to the snow, we present here results from varied snow samples from East Antarctica. First, we look at the relationship between isotopes and temperature from a geographical point of view, using results from three traverses across Antarctica, to see how the relationship is built up through the distillation process. We also take advantage of these measures to see how second order parameters (d-excess and 17O-excess are related to δ18O and how they are controlled. d-excess increases in the interior of the continent (i.e. when δ18O decreases, due to the distillation process, whereas 17O-excess decreases in remote areas, due to kinetic fractionation at low temperature. In both cases, these changes are associated with the loss of original information regarding the source. Then, we look at the same relationships in precipitation samples collected over one year at Dome C and Vostok, as well as in surface snow at Dome C. We note that the slope of the δ18O / T relationship decreases in these samples compared to those from the traverses, and thus advocate

  1. Monitoring the effects of exposure to lead and cadmium in working and living environment through standard biochemical blood parameters and liver endonucleases activity

    Directory of Open Access Journals (Sweden)

    Nikolić Ružica S.

    2011-01-01

    Full Text Available Heavy metals as pollutants in the working and living environment are a serious health and environmental problem because they are toxic, non-biodegradable, accumulate in living systems and have a long half-life in soil. Sources of lead contamination are combustion products in the chemical industry and metallurgy, industrial waste water, landfills, traffic etc. Lead enters into the body via the food chain and drinking water. In the body lead is deposited in the liver, kidneys, brain and mineral tissues. Excretion of lead causes damage to the epithelial cells of certain organs. High level exposure to cadmium is usually the result of environmental pollution by human activities. Exposure to cadmium can lead to acute and chronic tissue damage of various organs, including liver and kidneys in humans and in animals. In this paper we analyzed the effects of lead and cadmium exposure, in working and living environment, on the model system of experimental animals, particularly the activity of certain liver enzymes, acid and alkaline DNase, and standard biochemical blood parameters. The study showed that lead and cadmium significantly affect the protein content, red blood cells, hemoglobin and hematocrit, and the activity of liver enzymes. This harmful effect of this toxic metal can be reduced by the supplements.

  2. Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

    Directory of Open Access Journals (Sweden)

    Reka Tienken

    2015-09-01

    Full Text Available The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010 serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL, whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of energy to cows which were similar in body condition score had only minor effects on periparturient immune system function and the metabolism of those cows.

  3. Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows

    Directory of Open Access Journals (Sweden)

    Akalın Pınar Peker

    2015-09-01

    Full Text Available The aim of this study was to determine the ceruloplasmin (Cp and vitamin C concentrations, the total antioxidant status (TAS, and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV. Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL, whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST (P = 0.003 and Cp concentrations (P = 0.03 decreased (65.17 ± 5.03 and 7.70 ± 0.72 respectively in BLV-infected cows, as compared to healthy animals (100.67 ± 11.50 and 10.40 ± 0.70 respectively. A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unknown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.

  4. Blood haematological and biochemical parameters in normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) maintained in isothermic and isonutritional conditions

    Institute of Scientific and Technical Information of China (English)

    Anthony Sabasthin; Venkataswamy Girish Kumar; Sumanta Nandi

    2012-01-01

    Objective:The present study was envisaged to examine the hematological and biochemical, parameters in three different groups of buffaloes (regularly cycling, pregnant and repeat breeding) maintained in isothermic and isonutritional conditions to establish the variations in blood and/or serum components in these groups.Methods:Blood samples were analyzed for hemoglobin, PCV, TLC, neutophil, lymphocyte, eosinophil, and monocyte count, glucose, total protein, albumin, globulin, urea and cholesterol.Results:The results revealed significantly lower haemoglobin and packed cell volume value in repeat breeding when compared to pregnant and regularly cycling animals. The WBC value significantly higher in repeat breeding when compared to regularly cycling animals. The average mean values of neutrophils, lymphocyte and eosinophils revealed a no significant difference in the neutrophils, lymphocyte and eosinophils in between the group. The mean values of monocytes revealed a significantly higher value in repeat breeding animals when compared to pregnant animals. The serum glucose, total protein, cholesterol and urea levels were significantly lower in repeat breeding compared to pregnant and regularly cycling animals. The levels of albumin and globulin revealed non-significant difference among the groups.Conclusions: A significant decrease in the hemoglobin, PCV, glucose, total protein, cholesterol and urea was observed in the repeat breeding animals when compared to pregnant and regularly cycling animals.

  5. On the link between oil price and exchange rate: A time-varying VAR parameter approach

    International Nuclear Information System (INIS)

    The aim of this paper is to study the relationship between the effective exchange rate of the dollar and the oil price dynamics from 1976 to 2013. In this context, we propose to explore the economic literature dedicated to financial channels factors (exchange rate, monetary policy, and international liquidity) that could affect the oil price dynamics. In addition to oil prices and the effective exchange rate of the dollar, we use the dry cargo index as a proxy for the real economic activity and prices for precious and industrial raw materials. Using a Bayesian time-varying parameter vector auto-regressive estimation, our main results show that the US Dollar effective exchange rate elasticity of the crude oil prices is not constant across the time and remains negative from 1989. It then highlights that a depreciation of the effective exchange rate of the dollar leads to an increase of the crude oil prices. Our paper also demonstrates the growing influence of financial and commodities markets development upon the global economy. (authors)

  6. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation.; Efeito da radiacao gama em alguns parametros microbiologicos e bioquimicos da fermentacao alcoolica

    Energy Technology Data Exchange (ETDEWEB)

    Alcarde, Andre Ricardo

    2000-07-01

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  7. Influence of Dietary Zinc and Vitamin C Supplementation on Some Blood Biochemical Parameters and Egg Production in Free-Range Laying Hens

    Directory of Open Access Journals (Sweden)

    Vasko GERZILOV

    2015-03-01

    Full Text Available The study aimed to follow out the effect of antistress dietary supplements Zinteral 35 and vitamin C on the levels of some blood biochemical parameters (corticosterone, total cholesterol, glucose, total protein and creatinine and egg production in laying hens during cold (7o C, thermoneutral (19o C and hot (31o C periods. The fowls were divided in three groups (26 females and 3 males in each group. They were reared in a free-range management system with elements of organic production. The experimental treatments were as followed: first (control group without dietary supplement, second group with 100 mg Zinteral 35 per kg diet containing 35 mg/kg zinc oxide, third group with the same amount of Zinteral 35 together with 250 mg vitamin C per kg diet. During the three periods with different ambient temperature, the hens supplemented either with zinc alone (second group or co-administered zinc + vitamin C (third group had significantly lower levels of plasma corticosterone (P<0.001, serum cholesterol (P<0.05 and glucose (P<0.05 than those from the first (control group. The differences between the third and the first groups were bigger versus those between the second and the first groups. For the entire period (March 1 and June 21, egg production was higher by 2.22 % and 4.60 % in the second and third groups respectively in comparison to the first group. The combination of 100 mg Zinteral 35 and 250 mg vitamin C per 1 kg diet exhibited a synergistic effect in reducing cold and heat stress in laying hens and increased their egg production.

  8. Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio by Vitamin C and Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2015-12-01

    Full Text Available The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg/L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg/kg diet, vitamin C (1000 mg/kg diet or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA as well as biochemical parameters, including the activity of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, and acetylcholinesterase (AChE were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium-treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.

  9. Investigation of some biochemical parameters relating to energy metabolism in experimental rodent tumours after exposure to ionizing radiation and magnetic fields

    International Nuclear Information System (INIS)

    The work described in this thesis deals with the effects of ionizing and non ionizing radiation, upon rodent tumour metabolism and growth and comparable properties of B16 melanoma cell cultures. Metabolic processes investigated were related to energy production and possible repair mechanisms after radiation induced damage. Some effects of the drug mitoxantrone on energy yield were also studied 'in vivo' and 'in vitro'. The following sources of radiation were used: 100, 250 kVp of x-rays, 60Co gammas, 8 MV x-rays (from a linear accelerator), and magnetic fields of 0.5 Tesla (constant) and 5 x 10-3 Tesla/m (varying). The biochemical parameters measured were levels of adenosine-5'-triphosphate (ATP) isocitrate dehydrogenase (ICDH), glucose-6-phosphate dehydrogenase, (G6P-DH), phosphomannose isomerase (PMI) and tissue sulphydryl. This was done with tumours under normoxic and hypoxic conditions. Growth rate and nuclear magnetic resonance relaxation time constants T1 and T2 were measured in CaNT tumours. Their ultrastructural changes using electron micrographs were investigated after x-irradiation. Further, the effects of mitoxantrone upon ATP-production were measured in CaNT tumours. In addition the radiation response of B16 melanoma cells after exposure to 60Co gammas was studied, with and without treatment using mitoxantrone. Changes in levels of ATP and activities of G6P-DH and ICDH were observed at different times after irradiation in varying conditions studied, but no significant change in tissue sulphydryl levels was detected. Further it was shown in B16 cells that radiosensitivity could be modified using mitoxantrone. Tumor growth rate slowed with exposure to the magnetic fields

  10. Parasitological and biochemical parameters in Schistosoma mansoni-infected mice treated with methanol extract from the plants Chenopodium ambrosioides, Conyza dioscorides and Sesbania sesban.

    Science.gov (United States)

    Kamel, E G; El-Emam, M A; Mahmoud, S S M; Fouda, F M; Bayaumy, F E

    2011-12-01

    This study aims to detect the antischistosomal properties of the plants' Chenopodium ambrosioides, Conyza dioscorides and Sesbania sesban methanol extract against Schistosoma mansoni in infected mice, including determination of total protein and albumin levels and the activities of alanine and aspartate transaminases (AlT, AsT) and acid and alkaline phosphatases (AcP and AkP) enzymes in the serum of infected treated mice. Male Swiss albino mice were infected with S. mansoni and orally treated with methanol extract of the plants C. ambrosioides (1250 mg/kg/day), C. dioscorides and S. sesban (1000 mg/kg/day from each) for 2 consecutive days 7 weeks post infection (PI). In addition, treatment of mice with the tested dose of each plant extract was successively done (i.e. the 1st extract followed by the 2nd and 3rd one with an hour interval). Parasitological and biochemical parameters were assessed. Nine weeks PI, the reduction rates of worm load/mouse treated with either C. dioscorides (1000 mg/kg), C. ambrosioides (1250 mg/kg) or S. sesban (1000 mg/kg) were 40.9%, 53.7% and 54.4%, respectively. Successive treatment raised the reduction rates of worm load/mouse to 66.3% and the ova/g tissue in liver to 76.9%. Moreover, serum total protein and albumin levels and activities of AlT, Ast, AcP and AkP enzymes of infected treated mice were improved in comparison with those of infected untreated ones. It is concluded that administration of C. dioscorides, C. ambrosioides and S. sesban methanol extract to infected mice exhibited a moderate antischistosomal effect. Successive treatment improved the antischistosomal properties of these plant species, hence ameliorated the liver functions of treated mice that may suggest degenerations of liver granulomas and regenerative changes. PMID:21740980

  11. The effects of chronic betaine supplementation on exercise performance, skeletal muscle oxygen saturation and associated biochemical parameters in resistance trained men.

    Science.gov (United States)

    Trepanowski, John F; Farney, Tyler M; McCarthy, Cameron G; Schilling, Brian K; Craig, Stuart A; Bloomer, Richard J

    2011-12-01

    Trepanowski, JF, Farney, TM, McCarthy, CG, Schilling, BK, Craig, SA, and Bloomer, RJ. The effects of chronic betaine supplementation on exercise performance, skeletal muscle oxygen saturation, and associated biochemical parameters in resistance trained men. J Strength Cond Res 25(12): 3461-3471, 2011-We examined the effects of chronic betaine supplementation on exercise performance and associated parameters in resistance trained men. Men were randomly assigned in a double-blind manner using a crossover design to consume betaine (2.5 g of betaine mixed in 500 ml of Gatorade®) or a placebo (500 ml of Gatorade®) for 14 days, with a 21-day washout period. Before and after each treatment period, tests of lower- and upper-body muscular power and isometric force were conducted, including a test of upper-body muscular endurance (10 sets of bench press exercise to failure). Muscle tissue oxygen saturation (StO2) during the bench press protocol was measured via near infrared spectroscopy. Blood samples were collected before and after the exercise test protocol for analysis of lactate, nitrate/nitrite (NOx), and malondialdehyde (MDA). When analyzed using a repeated measures analysis of variance, no significant differences were noted between conditions for exercise performance variables (p > 0.05). However, an increase in total repetitions (p = 0.01) and total volume load (p = 0.02) in the 10-set bench press protocol was noted with betaine supplementation (paired t-tests), with values increasing approximately 6.5% from preintervention to postintervention. Although not of statistical significance (p = 0.14), postexercise blood lactate increased to a lesser extent with betaine supplementation (210%) compared with placebo administration (270%). NOx was lower postintervention as compared with preintervention (p = 0.06), and MDA was relatively unchanged. The decrease in StO2 during the bench press protocol was greater with betaine vs. placebo (p = 0.01), possibly suggesting

  12. Diagnosis and follow-up of idiopathic retroperitoneal fibrosis: role of (18)F-FDG-PET/CT and biochemical parameters in patients with renal involvement.

    Science.gov (United States)

    Fofi, Claudia; Prosperi, Daniela; Pettorini, Laura; Festuccia, Francescaromana; Pirisino, Riccardo; Lanni, Valerio; Scopinaro, Francesco; Punzo, Giorgio; Menè, Paolo

    2016-09-01

    Idiopathic retroperitoneal fibrosis (IRF) is a rare disease characterized by fibro-inflammatory reaction surrounding ureters and other inner organs with possible secondary renal involvement. Symptoms are aspecific and recurrent phases of activity are generally associated with elevation of inflammatory indices. 18F-FDG-PET is nowadays an important tool for the detection of this disease, allowing differentiation between metabolically active tissue and fibrotic one. The purpose of this study was to investigate the role of 18F-FDG-PET in the management of IRF and to evaluate possible correlations between biochemical parameters and PET/CT findings of disease activity. We enrolled seven consecutive patients with IRF (in five histology proved the disease) observed from 2003 to 2012 (5 M:2 F, mean age 53.8 years, range 44-86 years). All patients presented with fever as first symptom; two had obstructive renal failure requiring hemodialysis; one underwent monolateral nephrectomy for parenchyma infiltration; six presented ureteral involvement; three underwent ureteral stent placement. For each patient, during a mean total follow-up of 26.5 months we evaluated serum creatinine, BUN, Hb, RBCs, WBCs, PLT, CRP, ESR. Periodic 18F-FDG-PET/CT scans (every 5.9 months-mean) were performed in all patients. Statistical evaluation was performed using "stepwise regression" analysis. Steroids and immunosuppressive agents induced a progressive normalization of PET/CT scans in all patients at the end of follow-up. Stepwise regression analysis showed that BUN, serum creatinine and CRP only if considered together, significantly correlated with SUV max (p value = 0.000003057). 18F-FDG-PET is a useful tool for clinical decision making in patient with IRF, allowing to evaluate the efficacy of the pharmacological treatment and to detect early recurrences, to modify the therapeutic approach. Acute phase reactants are not reliable alone for the management and the follow-up as they are often

  13. Effects of Dietary L-carnitine Supplementation on Growth Performance, Organ Weight, Biochemical Parameters and Ascites Susceptibility in Broilers Reared Under Low-temperature Environment.

    Science.gov (United States)

    Wang, Y W; Ning, D; Peng, Y Z; Guo, Y M

    2013-02-01

    The objective of this study was to investigate the effects of L-carnitine on growth performance, organ weight, biochemical parameters of blood, heart and liver, and ascites susceptibility of broilers at different ages reared under a low-temperature environment. A total of 420 1-d-old male Ross 308 broilers were randomly assigned to two dietary treatments with fifteen replicates of fourteen broilers each. Treatment diets consisted of L-carnitine supplementation at levels of 0 and 100 mg/kg. At 11-d of age, low temperature stress was used to increase ascites susceptibility. Blood, heart and liver samples were collected at different ages for analysis of boichemical parameters. The results showed that, there was no significant difference in growth performance with L-carnitine supplementation, but the mortality due to ascites was significantly decreased. Dietary L-carnitine supplementation significantly reduced heart index (HI) and ascites heart index (AHI) on d 21, lung index (LUI) on d 35 and liver index (LI) on d 42. The broilers fed diets containing L-carnitine had significantly lower red blood cell counts (RBC), hemoglobin (HGB) concentration and hematocrit (HCT) on d 42. Dietary L-carnitine supplementation significantly reduced malondialdehyde (MDA) content of heart tissue on d 21 and 35, and significantly increased total superoxide dismutase (T-SOD) and Glutathione peroxidase (GSH-Px) activity of the heart on d 21 and 42. L-carnitine supplementation significantly reduced serum triglyceride (TG) content on d 28 and 35 and serum glucose (GLU) on d 35 and 42, and significantly increased serum total protein (TP) and globulin (GLO) content on d 42. L-carnitine supplementation significantly enhanced liver succinodehydrogenase (SDH), malic dehydrogenase (MDH) and Na(+)-K(+)-ATPase activity on d 28, and tended to reduce the lactic acid (LD) level of liver on d 35 (p = 0.06). L-carnitine supplementation significantly reduced serum uric acid (UA) content on d 28, 35 and 42

  14. A hepcidina como parâmetro bioquímico na avaliação da anemia por deficiência de ferro Hepcidin as a biochemical parameter for the assessment of iron deficiency anemia

    Directory of Open Access Journals (Sweden)

    Andrea dos Reis Lemos

    2010-01-01

    parameter for assessment of iron deficiency anemia is reported in this review. A literature review was carried out in the databases PubMed and LILACS, between 2006-2010, assessing hepcidin as a parameter for the regulation of iron metabolism. During this period 35 studies were published in international journals and one study in a Brazilian journal. The production of hepcidin is homeostatically regulated by anemia and hypoxia. When oxygen supply is inadequate the level of hepcidin decreases. Therefore, more iron from the diet and from the stock of macrophages and hepatocytes becomes available. Hepcidin links to ferroportin, regulating iron release to plasma. When the hepcidin concentrations are low, the molecules of ferroportin are exposed on the plasmatic membrane and release iron. When hepcidin levels increase, hepcidin binds to molecules of ferroportin inducing its internalization and degradation and iron release gradually decreases. Apparently, development of diagnosis and therapy for anemia based on the parameter hepcidin may provide a more effective approach. Large-studies are needed to demonstrate the importance of hepcidin for the differential diagnosis of anemia, including sample protocols for analysis, with standards similar to those used in other biochemical evaluations, as well as the definition of cut-off points for the plasma and urinary expression of this peptide.

  15. Hematological and biochemical parameters of Dorper ewesParâmetros hematológicos e bioquímicos de ovinos da raça Dorper

    Directory of Open Access Journals (Sweden)

    Karina Medici Madureira

    2013-05-01

    Full Text Available In order to establish hematological and biochemical Dorper sheep values and evaluate the influence of sex and age in these determinations, 72 animals were assigned to three experimental groups according to age: Group 1 (G1 consisting of animals up to 12 months of age (n=28, Group 2 (G2 composed of animals from 13 to 36 months (n=37 and Group 3 (G3 for animals from 37 to 60 months (n=7. The animals were still divided in another two groups according to gender: Group 4 (G4 – females (n=57 and Group 5 (G5 – males (n=15. Evaluating the results obtained for hematological and biochemical examinations it was found that the mean values of RBC (13.4 ± 2.5x106/mL, hemoglobin (12.6 ± 1.3 g/dL, packed cell volume (37.5 ± 3.7%, total leukocytes (7116.0 ± 1967.0/mL and segmented neutrophils (4168.0 ± 1501.0/mL were higher in the animals of G1, as well as urea, GGT and ALP (52.0 ± 17.0 mg/dL, 93.0 ± 19.0 IU/L, 257.0 ± 125.0 IU/L, respectively. Comparing the gender, it was found that the values of total leukocytes (7576.0 ± 2082.0/mL, segmented neutrophils (4296.0 ± 1510.0/mL and lymphocytes (2428.0 ± 1084.0/mL were greater in males (G5. The values of creatinine (1.3 ± 0.4 mg/dL, serum total protein (6.3 ± 0.9 g/dL and globulins (3.7 ± 1.0 g/dL were higher in females (G4. Statistical analysis of the results allowed to determine reference values for hematological and biochemical constituents of Dorper sheep breed, and concluded that there were variations in these parameters influenced by gender and different age groups. A fim de estabelecer os valores hematológicos e bioquímicos de ovinos da raça Dorper e avaliar a influência do sexo e idade nestas determinações, 72 animais foram distribuídos em três grupos experimentais de acordo com a faixa etária, sendo o Grupo 1 (G1 composto por animais com até 12 meses de idade (n=28, o Grupo 2 (G2 composto por animais de 13 a 36 meses de idade (n=37 e o Grupo 3 (G3 por animais de 37 a 60 meses de

  16. Assessment of air pollution from a lignite power plant in the plain of Megalopolis (Greece) using as biomonitors three species of lichens; impacts on some biochemical parameters of lichens

    International Nuclear Information System (INIS)

    Samples of three species of corticolous lichens (Anaptychia ciliaris, Lobaria pulmonaria and Ramalina farinacea) were collected from 22 Quercus spp. stands distributed in the plain (250 km2 of Megalopolis (Peloponnesos) southern Greece), and at various distances, elevations and directions with respect to a lignite power plant (550 MW), releasing pollutants from stacks of 110 and 180 m height. The concentrations of several metals (Ca, Cd, Cu, Fe, Mn, Pb, and Zn) and of total sulphur were determined in the thalli of the three species as well as the levels of a number of biochemical parameters. The statistical analysis of the data showed significant differences among the sites for the concentrations of elements and the biochemical parameters; multiple linear regression showed that the values of sulphur and most metals were partly due to the distance (negative effect), and elevation (positive effect) of the sites from the power plant. Significant differences were also found among the three species

  17. Effects of pollution on lead and cadmium concentration and correlation with biochemical parameters in blood of human population nearby Kosovo thermo power plants

    Directory of Open Access Journals (Sweden)

    L. Zeneli

    2008-01-01

    Full Text Available This study describes an investigation of lead and cadmium pollution of Kosovo environment as a result of outflow from the coal processing industry. In a comparative study of lead and cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants, (Obiliq a highly polluted environment and the other that is considered as relatively clean rural environment (Dragash. Analysis has shown that emission of particulate in fly ash from Thermo Power Plants during 2005 has exceeded EU standards by 400-500% and that lead concentration was 18mg kg-1 and cadmium concentration was -1 of ash. A series of determinations of lead and cadmium concentrations in blood of population that lives in this environment, have shown direct effects in biochemical parameters CRE (Creatinin, DB (Direct Bilirubine, TB (Total Bilirubine, AST (Aspartat Aminotransferaza, CK (Creatin Kinaza and CHE (Cholenisteraza in human organism. The results that were achieved in this study showed a significant difference in average lead and cadmium concentration in the blood of the investigated group of peoples that lives in the area near by the Power Plants, from a control group that lives in a rural unpolluted environment. Lead and cadmium has been analyzed in 50 samples taken from persons from industrial zone and 25 samples in controlled group. The level of lead concentration was 23.0-112.1 µg L-1in geometric average 46.05 µg L-1, cadmium concentration was 0.44-6.02 µg L-1 in geometric average of 1.56µg L-1. Controlled group from the rural relatively clean environment showed lead concentration of 6.7-33.8 µg L-1 in geometric avarage 17.76 µg L-1 and cadmium concentration of 0.21-1.8 µg L-1 or in geometric average of 0.73 µg L-1. In conclusion in exposed subjects, pollution from coal burning in Power Plant is very important factor for level of lead and cadmium concentration in blood of tested population.

  18. BIOCHEMICAL AND NON-SPECIFIC IMMUNE PARAMETERS OF HEALTHY NILE TILAPIA (Oreochromis niloticus), BLUE TILAPIA (Oreochromis aureus) AND THEIR INTERSPECIFIC HYBRID (MALE O. aureus × FEMALE O. niloticus) MAINTAINED IN SEMI-INTENSIVE CULTURE SYSTEM

    OpenAIRE

    Waleed N; El-Hawarry

    2012-01-01

    Oreochromis niloticus, Oreochromis aureus and their interspecific hybrid tilapia (♂ O. aureus x ♀ O. niloticus) maintained under semi-intensive culture system were compared in a preliminary study to explore the variations in blood biochemical and non-specific immunological parameters. Comparisons were performed after one week of acclimation (“base-line” level). Serum cholesterol, albumin, SGPT and SGOT level were significantly higher (P0.05) in total protein, globulin and urea. Additionally, ...

  19. Associations between annual and seasonal variations in body mass and reproductive success and blood biochemical parameters in semi-domesticated reindeer

    Directory of Open Access Journals (Sweden)

    Ingrid H. Holmøy

    2009-12-01

    Full Text Available The main objective of the study was to follow reproductive performance and blood biochemical parameters associated with fat and carbohydrate metabolism in a herd of free-ranging, semi-domesticated reindeer (Rangifer tarandus tarandus over a two-year period, with extreme between-year variation in forage availability. The effects of climatic factors on reindeer reproductive performance were investigated by analysing whether time of onset of luteal function in autumn and calf survival were associated with changes in body mass and weather conditions, such as snow depth, precipitation, and temperature. Considerable between-year variation in the onset of luteal activity was found. In 1997, 4.2% of the female reindeer were either cycling or pregnant in the second week of October, whilst in 1998, in the same week, 100% were cycling or pregnant. Although energy balance was important for timing of the onset of luteal activity, delayed conception had no apparent effect on calf survival. The results indicated that maternal body mass (BM in spring was of primary importance for calf survival, and the productivity of the herd. Since climatic factors influence the availability of forage, and hence female BM, it also has an indirect impact on calf survival. Females with low BM demonstrated greater seasonal variation in BM than heavier females. Plasma concentrations of free fatty acids and β-hydroxy butyric acid responded to changes in forage availability, but the initial condition of the reindeer and their fat reserves also seemed to have a major influence on these parameters.Abstract in Norwegian / Sammendrag:Sammenheng mellom års- og sesongvariasjon i kroppsvekt og henholdsvis reproduksjonssuksess og biokjemiske blodparametre hos tamreinHovedformålet med studien var å følge reproduksjonen og blodparametre knyttet til fett og karbohydratmetabolismen hos en tamreinflokk (Rangifer tarandus tarandus gjennom en toårs periode med stor årlig variasjon i n

  20. Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice: Part II: Impact on specific chemical and biochemical quality parameters

    NARCIS (Netherlands)

    Vervoort, L.; Plancken, van der I.; Grauwet, T.; Timmermans, R.A.H.; Mastwijk, H.C.; Matser, A.M.; Hendrickx, M.E.; Loey, van A.

    2011-01-01

    The impact of thermal, high pressure (HP) and pulsed electric field (PEF) processing for mild pasteurization of orange juice was compared on a fair basis, using processing conditions leading to an equivalent degree of microbial inactivation. Examining the effect on specific chemical and biochemical

  1. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A new analytical method for the determination of urea-urease system based on biochemical reaction heat induced laser beam deflection is presented in this paper. With the method, the Michaelis constant (Km) of urease and apparent inhibition constant (Ki) of some metal ion inhibitors were measured respectively. This method was also used for the quantitative determination of metal ions with satisfactory result.

  2. Performance Metrics and Design Parameters for a Free-space Communication Link Based on Multiplexing of Multiple Orbital-Angular-Momentum Beams

    CERN Document Server

    Xie, Guodong; Ren, Yongxiong; Huang, Hao; Yan, Yan; Ahmed, Nisar; Zhao, Zhe; Lavery, Martin P J; Ashrafi, Nima; Ashrafi, Solyman; Tur, Moshe; Molisch, Andreas F; Willner, Alan E

    2014-01-01

    We study the design parameters for an orbital angular momentum (OAM) multiplexed free-space data link. Power loss, channel crosstalk and power penalty of the link are analyzed in the case of misalignment between the transmitter and receiver (lateral displacement, receiver angular error, or transmitter pointing error). The relationship among the system power loss and link distance, transmitted beam size and receiver aperture size are discussed based on the beam divergence due to free space propagation. We also describe the trade-offs for different receiver aperture sizes and mode spacing of the transmitted OAM beams under given lateral displacements or receiver angular errors. Through simulations and some experiments, we show that (1) a system with a larger transmitted beam size and a larger receiver aperture is more tolerant to the lateral displacement but less tolerant to the receiver angular error; (2) a system with a larger mode spacing, which uses larger OAM charges, suffers more system power loss but les...

  3. Calorie restriction in biosphere 2: alterations in physiologic, hematologic, hormonal, and biochemical parameters in humans restricted for a 2-year period.

    Science.gov (United States)

    Walford, Roy L; Mock, Dennis; Verdery, Roy; MacCallum, Taber

    2002-06-01

    Four female and four male crew members, including two of the present authors (R. Walford and T. MacCallum)--seven of the crew being ages 27 to 42 years, and one aged 67 years--were sealed inside Biosphere 2 for two years. During seven eighths of that period they consumed a low-calorie (1750-2100 kcal/d) nutrient-dense diet of vegetables, fruits, nuts, grains, and legumes, with small amounts of dairy, eggs, and meat (approximately 12% calories from protein, approximately 11% from fat, and approximately 77% from complex carbohydrates). They experienced a marked and sustained weight loss of 17 +/- 5%, mostly in the first 8 months. Blood was drawn before entry into Biosphere 2, at many time-points inside it, and four times during the 30 months following exit from it and return to an ad libitum diet. Longitudinal studies of 50 variables on each crew member compared outside and inside values by means of a Bayesian statistical analysis. The data show that physiologic (e.g., body mass index, with a decrease of 19% for men and 13% for women; blood pressure, with a systolic decrease of 25% and a diastolic decrease of 22%), hematologic (e.g., white blood cell count, decreased 31%), hormonal (e.g., insulin, decreased 42%; T3, decreased 19%), biochemical (e.g., blood sugar, decreased 21%; cholesterol, decreased 30%), and a number of additional changes, including values for rT3, cortisol, glycated hemoglobin, plus others, resembled those of rodents or monkeys maintained on a calorie-restricted regime. Significant variations in several substances not hitherto studied in calorie-restricted animals are also reported (e.g., androstenedione, thyroid binding globulin, renin, and transferrin). We conclude that healthy nonobese humans on a low-calorie, nutrient-dense diet show physiologic, hematologic, hormonal, and biochemical changes resembling those of rodents and monkeys on such diets. With regard to the health of humans on such a diet, we observed that despite the selective

  4. Effect of Milk Thistle (Silybum marianum L. on Biochemical Parameters and Immunity of Broiler Chicks Fed Aflatoxin B1 after Three Weeks

    Directory of Open Access Journals (Sweden)

    Maliheh Amiri Dumari

    2014-09-01

    Full Text Available Background: This study was conducted to determine the efficacy of milk thistle seeds (MTSs in counteracting the toxic effects of aflatoxin B1 (AFB1 in a contaminated diet fed to broilers. Methods: Two dietary inclusion rates of AFB1 (0, 0.250 and 500 ppb and MTS (0, 0.5 and 1% were tested in a 3×3 factorial manner. The effect of nine experimental treatments was assessed using 216 one-d-old Ross 308 male broiler chicks in a randomized complete design with 4 replicates of 6 birds each from one to 21 days of age. The effects of dietary AFB1 and MTS on serum biochemistry factors, antibody titer against Newcastle disease (ND and influenza disease (ID in broilers were evaluated at the end of this period. Results: Statistical analysis of the main effects of diets indicated no significant changes in uric acid, cholesterol, triglycerides, low density lipoprotein (LDL, ID, and phosphorus compared to the control (P>0.01. Also, addition of 500 ppb of dietary AFB1 into the diet was associated with significant decreases in serum glucose, calcium, high density lipoprotein (HDL, and ND compared to the control group (P<0.01. The contaminated diet significantly increased the activities of aspartate aminotransferase (AST and alanine aminotransferase (ALT (P<0.05. Conclusion: Milk thistle showed protective effects and resulted in some serum enzyme activities and serum biochemical changes associated with aflatoxin toxicity.

  5. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose pblood biochemistry levels were observed statisticaly different pgroup. In conclusion, obese and obese+diabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.

  6. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose pdiabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.

  7. Hepato Protective Efficacy of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and Their Formulation on Imidacloprid Induced Liver Toxicity by Histopathological and Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    V. Eugin Amala

    2015-09-01

    Full Text Available Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations are used as a phytotherapeutic medicine for hepatic diseases. In the present study the effect of methanolic extract of herbal powder was administered against Imidacloprid in Albino Wistar Rats. Single dose of herbal (500 mg/kg/b.w was given orally for 28 days to Imidacloprid toxicity exposed to rats. They were observed each hour and each day for 28 days for any changes in behavioural activity loss of ability to move, cramping, lethargy, muscle weakness, convulsion, irritation of the eye, excess salivation, change in gait. Clinical observations body weight, organ weight, biochemical analysis and histopathological examination were carried out. Administration of Imidacloprid, produced liver degeneration such as cytoplasmic vacuolation, mild vacuolar degeneration, mild hepatic damage in low dose (40 mg/kg b.w and appearance of blood streaks, hepatic damage and severe vacuolar degenerations were observed in high dose of induction (80 mg/kg b.w. Animals were treated with the phytotherapeutic products of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations. Triphala extract was possessed more hepatoprotective activity, while comparing the four samples which was proved experimentally.

  8. Effect of treating lactating rats with lead acetate and its interaction with vitamin E or C on neurobehavior, development and some biochemical parameters in their pups

    Directory of Open Access Journals (Sweden)

    A. A. Hassan

    2010-01-01

    Full Text Available The current study investigated the effect of administration of vitamin E (600mg/ kg diet concomitantly with lead acetate (10mg/kg, orally and vitamin C (100mg/kg, orally concomitantly with lead acetate (10mg/kg, orally to the female lactating rats on the neurobehavioral, landmarks development and some biochemical tests in their pups. Administration of lead acetate to the female lactating rats caused a significant increase in open field activity test including (the number of squares crossed and rearing test within 3 minutes, olfactory discrimination test, triglycerides and malondialdehyde brain tissue, with a significant decrease in glutathione brain tissue and high density lipoproteins in their pups. The present study demonstrated that treatment of female lactating rats with vitamin C and lead acetate produced a significant decrease in righting reflex test in their pups. Administration of vitamin E concomitantly with lead acetate to the female lactating rats caused a significant increase in glutathione level accompanied with a significant decrease in malondialdehyde and triglycerides levels in their pups. The present study showed that treatment of female lactating rats with vitamin E or C with lead acetate produced a significant decrease in rearing test, whereas a significant increase in high density lipoproteins in their pups. It is concluded that administration vitamin E or C to the female lactating rats reverse the adverse effects produced by lead acetate on neurobehavioral. Vitamin E had positive effect on the levels of glutathione, malondialdehyde brain tissue, triglyceride and high density lipoproteins in their lactating pups.

  9. Effect of Calcium Soap of Fatty Acids Supplementation on Serum Biochemical Parameters and Ovarian Activity during Out-of-the-Breeding Season in Crossbred Ewes

    Directory of Open Access Journals (Sweden)

    Hayat H. M. El-Nour

    2012-01-01

    Full Text Available This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45–55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (<0.05 increases in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and progesterone levels with decrease in calcium and phosphorous levels in treated group. In treated group, normal-size ovaries and more than one follicle on the ovaries were detected and pregnancy rate increased. In conclusion, CSFA supplementation was effective to maintain the reproductive performance when ewes were out of the breeding season.

  10. [The effect of biotechnological treatment of milk-replacer feeding mixtures on hematologic and biochemical parameters and its utilization in calves].

    Science.gov (United States)

    Skrivanová, V; Machanová, L; Svoboda, T

    1989-10-01

    A trial was performed with cellulase administration (3 cx per g of ingested feed dry matter) to early weaned calves fed minimum amounts of milk replacer (21 kg per milk nutrition period) and sweet (C, D) and sour (A, B) diets. The groups B and D were supplemented with an enzyme till the age of 90 days. The calves were given free choice of COT concentrate mixture, hay and water. Liveweight gains, feed and nutrient intake and health condition of calves were the characteristics investigated in this trial. Hematocrit value, hemoglobin, glucose and urea contents were determined in blood, in blood plasma buffer capacity was investigated. Although the differences in calf performance are not statistically significant, the best growth of calves in the vegetable nutrition period was observed in calves in group D. Nutrient conversion was also higher in calves of group D in the vegetable nutrition period. In conversion of nutrient consumption per 1 kg liveweight gain the difference in the intake of digestible crude protein and starch units between the group of calves fed sweet milk without enzyme addition in the milk nutrient period (A--digestible crude protein 0.40 +/- 0.005; starch units 1.74 +/- 0.008) and group D (sour diet + 3 cx cellulase--digestible crude protein 0.34 +/- 0.007; starch units 1.52 +/- 0.044) was statistically significant (P less than 0.025 and P less than 0.05) in favour of group D. An analysis of the results of hematological and biochemical investigations during the trial demonstrates that the sour diet is better in view of changes in glucose concentrations.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2588429

  11. [b][/b]Impact of copper (Cu at the dose of 50 mg on haematological and biochemical blood parameters in turkeys, and level of Cu accumulation in the selected tissues as a source of information on product safety for consumers

    Directory of Open Access Journals (Sweden)

    Bogusław Makarski

    2014-09-01

    Full Text Available Introduction. The current state-of the art points to a positive impact of copper (Cu supplements on the general health status in poultry. Copper induces beneficial changes in the haematological and biochemical blood parameters. It also displays immunostimulating properties and helps maintain a proper microbiological balance in the digestive tract. [b]Objective[/b]. The objective of this study was to investigate the impact of Cu at the dose of 50 mg/kg BW, administered in organic and inorganic form, on the haematological and biochemical blood parameters and level of Cu bioaccumulation in the liver and pectoral muscle. [b]Materials and method[/b]. The study was carried out on 45 BUT-9 turkeys which had been were reared for 16 weeks. They were divided into 3 experimental groups: I – the control group; II – fed with CuSO[sub]4[/sub] at the dose of 50 mg Cu•dm [sup]-3 [/sup]H[sub]2[/sub]O; III – received a Cu chelate with lysine at the same dose. [b]Results[/b]. The administration of Cu at the dose exceeding the nutritional recommendations did not induce beneficial changes in the examined birds. This indicates that it is not necessary to administer Cu doses higher than the recommended levels. The extent of Cu accumulation in the pectoral muscle increased by 40% compared to the control group, whereas in the liver it was higher by 30–35% than in the birds without Cu administration. The level of Cu in tissues does not pose a risk to consumers. [b]Conclusions[/b]. The supplementation of Cu at the dose of 50 mg has a negative impact on the level of the analyzed parameters. The results of the presented study indicate that the administered Cu dose exceeds birds’ demand for this element.

  12. The suitability of some blood gas and biochemical parameters as diagnostic tools or early indicators of ascites syndrome in broiler sire lines.

    Science.gov (United States)

    Hasanpur, K; Nassiri, M R; Hosseini Salekdeh, G; Vaez Torshizi, R; Pakdel, A; Kermanshahi, H; Naghous, M

    2016-06-01

    In recent few years, there have been some attempts to find a reliable indicator trait as a selection criterion against susceptibility to ascites syndrome (AS). Blood parameters were of great interest as they could be measured in live animals without implementing an ascites-inducing challenge (AIC). In this work, the suitability of some blood parameters was evaluated for diagnosing AS-susceptible chicks in later steps of the disease in trial 1 as well as their early predictive ability in trial 2. In the first trial, one hundred 1-day-old chicks from two pure broiler lines namely S1 and S2 and, in the second trial, 226 1-day-old chicks from line S2 were subjected to AIC. Saline drinking water (1200 mg/l) and lower-than-standard ambient temperatures were the implemented AICs in trials 1 and 2 respectively. The blood parameters including pH, partial pressure of O2 (pO2 ), partial pressure of CO2 (pCO2 ), bicarbonate ion concentration (BIC), percentage of haematocrit (HCT) and saturated haemoglobin (SaO2 ) were measured twice per each bird at days 28 and 35 in trial 1 and once in trial 2 at day 21. The results of the first trial revealed that in line S2 some of the blood parameters differed significantly between the ascitic and non-ascitic groups following exposure to AIC. In this line, the incidence of AS was accompanied by a lower pO2 , SaO2 and BIC, while with higher pCO2 and HCT values. In the second trial, however, although almost all of the parameters showed meaningful differences between the ascitic and non-ascitic broilers, only mean difference of BIC parameter was statistically significant. The general conclusion of this study is that the blood parameters can somewhat have diagnostic ability in the condition in which the AIC is already present, whereas the results did not approve their usefulness as early predictors of AS. PMID:26608233

  13. Effect of some Physiological Parameters in Female Local Sheep on some Blood Biochemical Components and Hormonal Levels During Pregnancy and Lactation

    International Nuclear Information System (INIS)

    The present study was carried out to determine effect of breed, days of estimation within breed, parity number of ewes, sex and type of embryo and regression on age and weight of ewes, gestation length and some blood biochemical and hormones levels during pregnancy or post - partum period. A total number of 60 ewes, 2 - 4 years of age with body weight ranged between 48 to 58 kg from breeding flock in Research Farm of Nuclear Research Centre, Atomic Energy Authority were used in this study. And they were divided into two equal groups according to their breed (Ossimi and Rahmani breed ewes). Blood samples of approximately 7 ml were collected during pregnancy at 10, 20, 30, 50, 100, 120, 140, and 145 days up to parturitions. After parturitions blood samples were taken at 30 and 60 days. Serum total protein, albumin and globulin were determined. In addition, plasma triiodothyronine (T3) and thyroxin (T4) were determined. The results showed that Ossimi ewes had higher pregnancy duration (150.94 days) than Rahmani ewes (149.49 days), the shorter length was observed in ewes of the first parity while the higher of gestation length was found in ewes of the second parity, ewes having female fetus had a longest gestation length while the shortest gestation length was found in ewes having both male and female twins. It was found that during pregnancy period Rahmani ewes had higher T3 and T4 means than Ossimi ones T3 and T4 plasma triiodothyronine (T3) level decreased up to 50 th day of the pregnancy period in Ossimi and Rahmani ewes then it sharply increased at the 145 th day of pregnancy period. In addition, plasma T3 level decreased significantly with increasing ewes parity. The highest level of plasma T3 level (73.50 ng/dl) was found in ewes having twins (male and female) fetus while those having female and having male were found to be 66.50 and 63.15 ng/dl, respectively. Ewes having single had higher values of T3 level compared with twins while the T4 levels concentration

  14. Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus) experimentally infected with Trichinella zimbabwensis

    OpenAIRE

    Louis J. La Grange; Samson Mukaratirwa

    2014-01-01

    Fifteen crocodiles were randomly divided into three groups of five animals. They represented high-infection, medium-infection and low-infection groups of 642 larvae/kg, 414 larvae/kg and 134 larvae/kg bodyweight, respectively. The parameters assessed were blood glucose, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT). The humoral immune response to Trichinella zimbabwensis infection was evaluated in all three groups by an ...

  15. Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus fingerlings

    Directory of Open Access Journals (Sweden)

    Shao Wei Zhai

    2014-07-01

    Full Text Available The present study was performed with tilapia (Oreochromis niloticus to evaluate the effects of diet supplementation with grape seed proanthocyanidins (GSPs on fish growth performance, some serum parameters and body composition. Three hundred tilapia fingerlings with the initial average body weight of 9.50±1.25 g were randomly divided into five treatment groups with four replicates in each group and 15 fish in each replicate. The dietary GSPs levels of five treatment groups were 0 (control group, 200, 400, 600, and 800 mg/kg, respectively. The trial period was 49 days. Growth performance parameters were significantly improved by GSPs supplementation (P<0.05, while survival rates were similar among all groups (P>0.05. Serum parameter results showed that activities of aminotransferase aspartate in 200 and 400 mg/kg GSPs groups and alanine aminotransferase in 400 mg/kg GSPs group were lowered significantly (P<0.05. Levels of triglyceride and total cholesterol (except 200 mg/kg GSPs group were significantly lowered, while lysozyme activity and albumin level were significantly higher in fish of GSPs supplemented groups, independently from the level of supplementation. The highest crude protein level and lowest crude lipid level were found in fish of all GSPs supplemented groups, while levels of moisture and ash in fish of all groups were similar (P>0.05. The results indicated that dietary 200 mg/kg GSPs could exert beneficial effects on growth and body composition of tilapia fingerlings, and ameliorate serum biochemistry parameters related to health status.

  16. Leaf photosynthesis and respiration of three bioenergy crops in relation to temperature and leaf nitrogen: how conserved are biochemical model parameters among crop species?

    OpenAIRE

    2011-01-01

    Given the need for parallel increases in food and energy production from crops in the context of global change, crop simulation models and data sets to feed these models with photosynthesis and respiration parameters are increasingly important. This study provides information on photosynthesis and respiration for three energy crops (sunflower, kenaf, and cynara), reviews relevant information for five other crops (wheat, barley, cotton, tobacco, and grape), and assesses how conserved photosynt...

  17. Body Composition, Hemodynamic and Biochemical Parameters in Young Female Normal-Weight Oligo-amenorrheic and Eumenorrheic Athletes and Non-athletes

    Science.gov (United States)

    Singhal, Vibha; de Lourdes Eguiguren, Maria; Eysenbach, Lindsey; Clarke, Hannah; Slattery, Meghan; Eddy, Kamryn; Ackerman, Kathryn E.; Misra, Madhusmita

    2014-01-01

    Aims Low-weight hypogonadal conditions such as anorexia nervosa are associated with marked changes in body composition, hemodynamic and hematological parameters, and liver enzymes. The impact of athletic activity in normal-weight adolescents with/without amenorrhea on these parameters has not been assessed. Our aim was to examine these parameters in normal-weight athletes and non-athletes and determine any associations of body composition, oligo-amenorrhea and exercise intensity. Methods We assessed vital signs, complete blood counts, liver enzymes, and regional body composition in 43 oligo-amenorrheic athletes (OAA), 24 eumenorrheic athletes (EA) and 23 non-athletes 14-21 years of age. Results BMI was lower in OAA than EA. Systolic and pulse pressure, and temperature were lowest in OAA. Blood counts did not differ among groups. AST was higher in both groups of athletes, while ALT was higher in OAA than EA and non-athletes. Total and regional fat was lower in OAA than other groups, positively associated with heart rate and inversely with liver enzymes. Conclusions Athletic activity is associated with higher AST, whereas menstrual dysfunction is associated with lower total and regional fat and higher ALT. Higher liver enzymes are associated with reductions in total and regional fat. PMID:25376841

  18. 辐射传输模型多尺度反演植被理化参数研究进展%Review of inversing biophysical and biochemical vegetation parameters in various spatial scales using radiative transfer models

    Institute of Scientific and Technical Information of China (English)

    肖艳芳; 周德民; 赵文吉

    2013-01-01

    Vegetation is one of the most important components of the earth ecosystem,and its biophysical and biochemical parameters are closely correlated to many exchanges of energy and matter in natural environments.So estimation of the key biophysical and biochemical variables accurately is critical for many ecological,agronomic,and meteorological applications,such as monitoring the growth condition of vegetation,warning the forest fire and studying the global carbon-nitrogen cycle.Empirical-statistical approach and physically based canopy reflectance model are the two main methods to estimate vegetation characteristics.Since the vegetation reflectance is impacted by several internal and external factors,which are different both spatially and temporally according to the different vegetation types.There is a critical issue on the empiricalstatistical approach due to its lacking of generality.And the empirical-statistical approach will be site-,time-and cropspecific because of the existed relationship of reflectance and its established biochemical and biophysical parameters.The vegetation radiative transfer models,as the most important physically based model,are established on fundamental theories of mathematics,physics and biology,and they describe the transfer and interaction of radiation inside the canopy based on physical laws.Many researchers have proved the radiative transfer models as an important tool to understand and quantify the relationship between vegetation object properties and remotely detected radiance signals,with their strong stability and good spatiotemporal transferability by comparing with the empirical-statistical approach.On the basis of different vegetation types and research objectives,a variety of radiative transfer models have been developed for different research purpose in the past two decades,and there are many reports on the application of these models.In this review,we focused on introducing the vegetation radiative transfer models used to

  19. Modelling the anaerobic digestion of solid organic waste - Substrate characterisation method for ADM1 using a combined biochemical and kinetic parameter estimation approach.

    Science.gov (United States)

    Poggio, D; Walker, M; Nimmo, W; Ma, L; Pourkashanian, M

    2016-07-01

    This work proposes a novel and rigorous substrate characterisation methodology to be used with ADM1 to simulate the anaerobic digestion of solid organic waste. The proposed method uses data from both direct substrate analysis and the methane production from laboratory scale anaerobic digestion experiments and involves assessment of four substrate fractionation models. The models partition the organic matter into a mixture of particulate and soluble fractions with the decision on the most suitable model being made on quality of fit between experimental and simulated data and the uncertainty of the calibrated parameters. The method was tested using samples of domestic green and food waste and using experimental data from both short batch tests and longer semi-continuous trials. The results showed that in general an increased fractionation model complexity led to better fit but with increased uncertainty. When using batch test data the most suitable model for green waste included one particulate and one soluble fraction, whereas for food waste two particulate fractions were needed. With richer semi-continuous datasets, the parameter estimation resulted in less uncertainty therefore allowing the description of the substrate with a more complex model. The resulting substrate characterisations and fractionation models obtained from batch test data, for both waste samples, were used to validate the method using semi-continuous experimental data and showed good prediction of methane production, biogas composition, total and volatile solids, ammonia and alkalinity. PMID:27156366

  20. Protective Effects of Vitamin C (Ascorbic Acid in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

    Directory of Open Access Journals (Sweden)

    Alireza AYOUBI

    2015-01-01

    Full Text Available The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42 were randomly assigned into7 groups: I healthy; II fed lead acetate only; III vitamin C administered only; IV diabetic; V diabetic rats administered by vitamin C; VI diabetic rats given lead acetate and VII diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

  1. Corneal Biomechanical and Anterior Chamber Parameters Variations after 1-year of Transepithelial Corneal Collagen Cross-linking in Eyes of Children with Keratoconus

    Science.gov (United States)

    Salman, Abdelrahman Gaber

    2016-01-01

    Aim: To assess the changes in corneal hysteresis (CH) and corneal resistance factor (CRF) 1-year following transepithelial corneal collagen cross-linking (CXL) treatment in eyes of children with keratoconus. Methods: This case series was conducted in 22 eyes of 22 children. Children aged 0.05). Conclusion: Transepithelial CXL in keratoconus in pediatric age group seems to have good stability in corneal biomechanical parameters after 1-year. Further studies with a larger sample and better study design are recommended to confirm our findings. PMID:26957852

  2. Linking Soil Physical Parameters Along a Density Gradient in a Loess-Soil Long-Term Experiment

    DEFF Research Database (Denmark)

    Eden, Marie; Møldrup, Per; Schjønning, Per;

    2012-01-01

    It is important to understand the impact of texture and organic carbon (OC) on soil structure development. Only few studies investigated this for silt-dominated soils. In this study, soil physical properties were determined on samples from a controlled experiment (Static Fertilization Experiment......, Bad Lauchstadt, Germany) on a loess soil that started more than 100 years ago with six different combinations of organic and mineral fertilizers. The parameters measured include soil texture, water retention curve, air-connected porosity, gas diffusion coefficient, air permeability, and saturated...... was highly similar to the total air-filled porosity calculated from gravimetric water content. For all six treatments, diffusivities and permeabilities were quite similar; both suggested that air-filled pore space was inactive for gas transport for air saturation below 0.1, but became highly connected...

  3. Effects of diets with increasing levels of citrus pulp on the blood parameters linked to energy metabolism in horses

    Directory of Open Access Journals (Sweden)

    Madalena Lima Menezes

    2014-12-01

    Full Text Available Currently, the use of alternative energy ingredients for horses has increased because these foods contain "superfibers", making safer diet for these animals. To study the influence of diets containing increasing levels of citrus pulp on albumin, triglycerides, cholesterol, glucose, insulin and short chain fatty acid (SCFA concentrations in the blood, five animals were used, with an average age of 3.5 years and a live weight of 460.66±76.86 kg, they were fed twice a day, at 7:00 am and 4:00 pm. The diets were formulated to meet the requirements of the animals at maintenance. 60% of the energy was obtained from forage, and 40% from concentrate, containing the following inclusion levels of citrus pulp (0, 7, 14, 21 and 28%. The area under the curve (AUC and the glucose and insulin peaks were calculated, and no differences were observed between the treatments. There was no effect of diet on the blood concentrations of the evaluated parameters tested, and there was no effect of the time of collection on the following variables: SCFAs, cholesterol, triglycerides and albumin. However, the diet did have quadratic effect on the glucose (Ŷ =-0.5327X²+4.2130X+84.5276 and insulin (Ŷ=-0.1002X² +0.8233X + 1.6336 concentrations. Up to 28% of the concentrate can be composed of citrus pulp in horse diets without causing any alterations on the concentrations of the parameters analyzed in the blood. High-fiber diets with easily fermentable fibers are beneficial because they maintain the glucose and insulin curves close to the baseline levels.

  4. APPLICATION OF SWARM INTELLIGENCE PSO TECHNIQUE FOR ANALYSIS OF MULTIMEDIA TRAFFIC AND QOS PARAMETERS USING OPTIMIZED LINK STATE ROUTING PROTOCOL

    Directory of Open Access Journals (Sweden)

    Dr. S. Meenakshi Sundaram

    2015-03-01

    Full Text Available Mobile ad hoc network (MANET nodes include wireless transmitters and receivers. At a given point in time, depending on the positions of the nodes, their transmitter and receiver coverage patterns, communication power levels and co-channel interference levels, a wireless connectivity in the form of a random, multi hop graph or “ad hoc" network exists among the nodes. In this research, it is proposed to modify OLSR using swarm intelligence, Particle Swarm Optimization (PSO, to reduce end to end delay and improve throughput in the network by traffic shaping at the network layer. The PSO algorithm represents each solution as a ‘bird’ in the search space and is referred to as ‘particle’. It uses the objective function to evaluate its candidate solutions, and operates on the resultant fitness values. Candidate solution and its estimated fitness, and velocity give the position of the particle. It also remembers the best fitness value it achieved till then during the algorithm’s operation which is usually referred to as the individual best fitness, and the candidate solution that achieved this fitness, is the individual best position ‘pbest’. The best fitness value attained among all particles in the swarm which is called global best fitness, and the candidate solution that attained this fitness, which is called the global best position or global best candidate solution ‘gbest’. OLSR generates link state information through nodes elected as Multi Point Relays (MPRs. It is proposed to modify OLSR using particle swarm optimization to reduce end to end delay and improve network throughput.

  5. Assessing anaerobic co-digestion of pig manure with agroindustrial wastes: the link between environmental impacts and operational parameters.

    Science.gov (United States)

    Rodriguez-Verde, Ivan; Regueiro, Leticia; Carballa, Marta; Hospido, Almudena; Lema, Juan M

    2014-11-01

    Anaerobic co-digestion (AcoD) is established as a techno-economic profitable process by incrementing biogas yield (increased cost-efficiency) and improving the nutrient balance (better quality digestate) in comparison to mono-digestion of livestock wastes. However, few data are available on the environmental consequences of AcoD and most of them are mainly related to the use of energy crops as co-substrates. This work analysed the environmental impact of the AcoD of pig manure (PM) with several agroindustrial wastes (molasses, fish, biodiesel and vinasses residues) using life cycle assessment (LCA) methodology. For comparative purposes, mono digestion of PM has also been evaluated. Four out of six selected categories (acidification, eutrophication, global warming and photochemical oxidation potentials) showed environmental impacts in all the scenarios assessed, whereas the other two (abiotic depletion and ozone layer depletion potentials) showed environmental credits, remarking the benefit of replacing fossil fuels by biogas. This was also confirmed by the sensitivity analysis applied to the PM quality (i.e. organic matter content) and the avoided energy source demonstrating the importance of the energy recovery step. The influence of the type of co-substrate could not be discerned; however, a link between the environmental performance and the hydraulic retention time, the organic loading rate and the nutrient content in the digestate could be established. Therefore, LCA results were successfully correlated to process variables involved in AcoD, going a step further in the combination of techno-economic and environmental feasibilities. PMID:25150742

  6. Effect of priming on growth, biochemical parameters and mineral composition of different cultivars of coriander (Coriandrum sativum L. under salt stress

    Directory of Open Access Journals (Sweden)

    Ben Fredj Meriem

    2014-08-01

    Full Text Available At Higher Institute of Agriculture of Chott Mariem, Sousse, Tunisia, this study was conducted to evaluate the interactive effect of salinity and seed priming on coriander. The experiment was carried out in completely randomized design with three replications consisting of four coriander genotypes (Tunisian cv, Algerian cv, Syrian cv and Egyptian cv at two seed conditions (seed priming with 4 g/l NaCl for 12h or no seed priming. Results revealed that seed priming and salinity had significantly (p≤0.05 affected all the parameters under study. On the first hand, salinity stress had adversely affected growth, chlorophyll content, mineral composition (K+ and Ca2+ of coriander in all genotypes. Also, it activated Na+ accumulation and synthesis of proline, soluble sugars and proteins. However, seed priming with NaCl had diminished the negative impact of salt stress in all cultivars and primed plants showed better response to salinity compared to unprimed plants. Maximum values were recorded in tolerant cultivar which is Tunisian one whereas minimum values were noted in sensitive cultivar (Algerian cv.

  7. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  8. Improvement of biochemical parameters in type 1 diabetic rats after the roots aqueous extract of yacon [Smallanthus sonchifolius (Poepp.& Endl.)] treatment.

    Science.gov (United States)

    Oliveira, Gilberto Ornelas; Braga, Camila Pereira; Fernandes, Ana Angélica Henrique

    2013-09-01

    The aim of this study was to evaluate the effect of yacon (Smallanthus sonchifolius) (Poepp.& Endl.) on clinical parameters under diabetic conditions. The aqueous extract of yacon tuberous roots (YRAE; 0.76 g fructan kg⁻¹ body weight) was prepared at the moment of each administration. Thirty-two male rats were divided into four groups (n=8): control group (C); group that received YRAE (Y); untreated diabetic group (DM1); and diabetic group treated with YRAE (Y-DM1). The diabetes mellitus was induced by streptozotocin (60 mg kg⁻¹ body weight). The animals from Y2 and Y-DM1 received YRAE by gavage, at 7-day intervals, for 30 days. The aqueous extract of yacon roots decreased (p0.05) between the Y and Y-DM1 groups. YRAE normalised alanine aminotransferase (ALT) activity, when comparing DM1 and Y-DM1 rats, but had no effect on lactate dehydrogenase activity (LDH). In conclusion, YRAE was sufficient for controlling water and food consumption, hyperglycaemia and dyslipidaemia, and promote the reduction of the ALT, suggesting a hepatoprotective effect in rats with STZ-induced DM1. PMID:23770327

  9. The effects of different levels of Chlorella microalgae on blood biochemical parameters and trace mineral concentrations of laying hens reared under heat stress condition

    Science.gov (United States)

    Moradi kor, Nasroallah; Akbari, Mohsen; Olfati, Ali

    2016-05-01

    This study was conducted to investigate the effect of different supplementation levels of Chlorella microalgae on serum metabolites and the plasma content of minerals in laying hens reared under heat stress condition (27.5-36.7 °C, variable). A total number of 378 (40 weeks of age, with mean body weight of 1390 ± 120 g) were randomly allocated to six treatments with seven replicates. The birds were randomly assigned to 6 treatments (C, T1, T2, T3, T4, and T5) with 7 replicate cages of 9 birds. C. microalgae at the rates of 100, 200, 300, 400, and 500 ppm with water were offered to groups T1, T2, T3, T4, and T5, respectively, while group C served as a control. At 71 days of trial, blood samples (14 samples per treatment) were taken for measuring serum metabolites and at 72 days for plasma mineral analysis. The results of this experiment showed that the supplementation of 200-500 ppm C. microalgae decreased the serum content of cholesterol, triglycerides, and LDL ( P blood parameters of laying hens reared under heat stress.

  10. A new multiple scattering scheme for the FLAIR forest radiative transfer model: Application to biochemical and biophysical parameter retrieval using hyperspectral data

    Science.gov (United States)

    Omari, Khalid

    This thesis investigated the development and assessment of a simple parameterization of the multiple scattering within canopies assuming the single scattering field is known and the background beneath the canopy is completely absorbing. The parameterization is based on the concept of spectral invariants related to recollision and escape probabilities from vegetation canopies. The simplified approach is evaluated against detailed 3-D ray tracing model, PARCINOPY, as well as reference datasets from the Radiation Modelling Intercomparison Experiment On-Line Checker. Comparison with homogenous canopies simulated with PARCINOPY showed that the model's performance is best in both the solar principal and perpendicular planes at low and mid LAI levels for all solar zenith angles. The comparison to the On-line Checker datasets shows also that the model is a suitable approach to describe the multiple scattering components of physically based models. This simple parameterization is then incorporated into the Four Scale Linear Model for Anisotropie Reflectance (FLAIR) canopy radiative transfer model to enhance the description of the spectrally dependant multiple scattered radiation field of a forest canopy. The contribution of the multiply scattered radiation between the canopy and the background is also added to the parameterization of the multiple scattering component. The validation of the new version of the FLAIR model was performed using the multi-angular data sets obtained by the airborne sensor POLarization and Directionality of the Earth's Reflectances (POLDER) during the BOReal Ecosystem-Atmosphere Study (BOREAS) campaign of 1994. The results indicate that this approach is well suited to the FLAIR model. It is also demonstrated that the multiple scattering problem can be parameterized by a limited number of architectural parameters and the leaf scattering coefficient. Finally, the combined canopy-leaf PROFLAIR (PROSPECT + FLAIR) model is used to investigate the

  11. A LINK TO THE PAST: USING MARKOV CHAIN MONTE CARLO FITTING TO CONSTRAIN FUNDAMENTAL PARAMETERS OF HIGH-REDSHIFT GALAXIES

    International Nuclear Information System (INIS)

    We have developed a new method for fitting spectral energy distributions (SEDs) to identify and constrain the physical properties of high-redshift (4 2'. It allows us to compare observations to arbitrarily complex models and to compute 95% credible intervals that provide robust constraints for the model parameters. The work is presented in two sections. In the first, we test πMC2 using simulated SEDs to not only confirm the recovery of the known inputs but to assess the limitations of the method and identify potential hazards of SED fitting when applied specifically to high-redshift (z > 4) galaxies. In the second part of the paper we apply πMC2 to thirty-three 4 6 galaxies. Using πMC2, we are able to constrain the stellar mass of these objects and in some cases their stellar age and find no evidence that any of these sources formed at a redshift larger than z = 8, a time when the universe was ≈0.6 Gyr old.

  12. Parâmetros clínicos, hematológicos, bioquímicos e hormonais de bovinos submetidos ao estresse calórico Clinical, hematological, biochemical, and hormonal parameters of cattle submitted to heat stress

    Directory of Open Access Journals (Sweden)

    F. Ferreira

    2009-08-01

    Full Text Available Caracterizaram-se as respostas clínicas, hematológicas, bioquímicas e hormonais de 71 bovinos F2 (½ Gir x ½ Holandês, 39 machos e 32 fêmeas, entre 14 e 20 meses de idade, submetidos a condições de termoneutralidade (22ºC a 70% de umidade relativa e ao estresse calórico (42ºC e 60% de umidade relativa. Os tratamentos foram realizados nas estações de verão e inverno em câmara bioclimática. Ao exame clínico, todos os animais apresentaram salivação e retração de globo ocular. Os animais apresentaram aumento do número de eritrócitos, concentração de hemoglobina, hematócrito, proteínas totais, ureia, creatinina, sódio, potássio, cloretos, cortisol, pH e densidade urinária e matéria seca das fezes no período de estresse calórico (P0,05. Concluiu-se que as variáveis hematológicas, bioquímicas e hormonais, bem como as características fenotípicas, revelaram-se importantes para caracterizar o estresse calórico em bovinos.Clinical, haemathological, biochemical, and hormonal parameters were characterized in 39 males and 32 females crossbred cattle (½ Holstein x ½ Gir, averaging 14 to 20 months of age, submitted to thermoneutrality (22ºC and 70% of RH and heat stress (42ºC and 60% RH in summer and winter in climatic chamber. At clinical examination, all animals presented salivation and ocular globe retraction. Under heat stress, animals increased erythrocytes count, hemoglobin total concentration, hematocrit, concentraction of total proteins, urea, creatinin, sodium, potassium, chlorides, cortisol, urinary pH and density, and dry matter of feces (P0.05. It was concluded that hematology, biochemical, and hormonal parameters are important to characterize the heat stress in cattle.

  13. Optimization of Sorption Parameters for Color Removal of Textile Dye by Cross-linked Chitosan Beads Using Box-Behnken Design

    Directory of Open Access Journals (Sweden)

    Jawad Ali H.

    2016-01-01

    Full Text Available Cross-linked chitosan-epichlorohydrin beads (CEB was prepared and studied for the removal of Reactive Red 4 (RR4 from textile industry. The effect of parameters such as adsorbent dosage (A: 0.5-1.5 g/L, pH (B: 4-10 and time of contact (C: 30-80 min on the color removal of RR4 was investigated using response surface methodology (RSM based on Box–Behnken surface statistical design at an initial RR4 concentration, Co = 100 mg/L as a fixed input parameter. The results of RSM indicated that the proposed model predicted the responses adequately within the limits of input parameters being used. Regression analysis showed good fit of the experimental data to the second-order polynomial model with coefficient of determination (R2 value of 0.9958 and Fisher F-value of 185.36. Optimization of adsorbent dose (1.0 g/L, pH (4.0, and contact time (80 min gave a maximum of 70.53% RR4 removal by CEB.

  14. Aircraft noise: Changes of biochemical parameters

    Energy Technology Data Exchange (ETDEWEB)

    Marth, E.; Moese, J.R.; Gallasch, E.; Fueger, G.F.

    1988-01-01

    The effect of stress caused by aircraft noise was studied on 14 female and 11 male volunteers, who were of a age ranging from 21 to 42 years and of a mean age of 25 years. The volunteers were exposed to an aircraft simulator the simulated the lowlevel flight of an airforce plane and produced a maximum noise level of 105 dB(A) for 3 sec. in a short time. Before and immediately after the exposure, the concentration of ACTH was measured by means of a radioimmunoassay. The ACTH is a hormone, responsible for initiating a chain-reaction that is characteristic for a stress reaction. In 100% of the cases the concentration of this hormone increased. It reached a pathological level in 28% of the cases. The effect on the lipid metabolism was expressed by an increase of total cholesterol and a decrease of the triglycerides in the serum. A slight increase in blood sugar which, together with the free fatty acids, is relatively quickly reduced to energy, could determined. The aircraft noise did not influence the activity of the liver transaminases in any way. A short-term exposure to aircraft noise is able to stimulate a stress reaction, whereby, the determination of the ACTH offers valuable informations.

  15. 三种复方中草药对斑点叉尾鮰部分生化指标的影响%Effects of Three Compound Chinese Herbal Medicines on Some Biochemical Parameters in Ictalurus punctatus

    Institute of Scientific and Technical Information of China (English)

    郭永军; 乔之怡; 胡玉花; 陈成勋; 白东清; 丁莉莉; 刘志伟; 邢克智

    2012-01-01

    In order to test the effects of compound Chinese herbal medicine "Yuhuan", "Gantaile" and "Sanhuangfen" on biochemical parameters in Ictalurus punctatus, an oral administration trial was performed. The medicines mentioned above were added to a commercial feed at 5 g/kg separately, and were administrated to the tested fish. The fish was sampled after a continuous 7-day feeding. The biochemical parameters in blood and tissues were assayed. The results show that all the medicines enhanced SOD/MDA and CAT/MDA ratio in serum and spleen. So the antioxidant capacity in serum and spleen was enhanced. All the medicines reduced the antioxidant capacity in head kidney by means of reducing SOD/MDA and CAT/MDA ratio. The "Gantaile" and "Yuhuan" enhanced the antioxidant capacity in gill, while "Sanhuangfen" and "Yuhuan" enhanced the antioxidant capacity in muscle. All the medicines reduced AST and ALT activities in hepato-pancreas. The LZM activity was significantly cut down in hepato-pancreas by "Gantaile" and in head kidney by "Yuhuan", while "Sanhuangfen" boosted LZM activity in muscle significantly. All the medicines enhanced blood leucocytes phagocytosis and reduced AST and ALT activities in hepato-pancreas.%为研究复方中草药“鱼欢”、“肝泰乐”、“三黄粉”对斑点叉尾鮰生化指标的影响,将以上3种中草药按5g/kg饵料的比例加到饵料中,连续喂食斑点叉尾鮰7d后取样,测定血液和组织中的生化指标。结果表明:3种复方中草药均能提高血清和脾脏的SOD/MDA和cAz孙佃A比值,提高血清和脾脏的抗氧化能力;3种复方中草药均能降低头肾的SOD/MDA和CAT/MDA比值,降低其抗氧化能力。“肝泰乐”和“鱼欢”能提高鮰的抗氧化能力,“三黄粉”和“鱼欢”能提高肌肉的抗氧化能力。3种复方中草药能降低肝胰脏中AST和ALT的活力。“肝泰乐”显著降低肝胰脏的LZM活力,“鱼欢”显著降低头肾的LZM活力

  16. The changes of hematology and biochemical parameters in the beef cattle with subacute ruminal acidosis%肉牛亚急性瘤胃酸中毒血液学及生化指标变化分析

    Institute of Scientific and Technical Information of China (English)

    赵晨旭; 王晓旭; 孙国权; 张良; 陈灰; 杨文涛; 刘兆喜; 王哲; 刘国文

    2013-01-01

    为了解肉牛发生亚急性瘤胃酸中毒时血液学和相应生化指标的变化,对瘤胃液pH在5.2~5.5、5.6~5.8和5.9~6.8之间的24头肉牛进行了血液学及相应生化指标的分析.结果表明:随着pH值的降低,血液中红细胞(RBC)数量、血红蛋白(Hb)浓度、红细胞压积(PCV)均显著增加;白细胞(WBC)总数上升,白细胞分类(WBC-DC)也有相应的变化,嗜酸性粒细胞和淋巴细胞呈下降趋势,中性杆状核粒细胞和中性分叶核粒细胞呈上升趋势,单核细胞略微升高;血气指标pO2显著增加,pCO2、HCO3-浓度显著减少;生化指标Ca2+、Cl-显著减少,Na+和尿素氮显著增加,K+差异不显著.肉牛发生轻度和中度亚急性瘤胃酸中毒时,pCO2以及HCO3-含量显著降低,表明机体碱储备不足,缓冲能力下降,存在代偿性酸中毒;同时根据水合状态和血象指标,表明亚急性瘤胃酸中毒病牛存在轻度脱水和炎性反应.%The aim of this study was to investigate the changes of hematology and biochemical parameters in beef cattle with subacute ruminal acidosis. We analyzed the hematology and biochemical parameters of 24 beef cattle with ruminal pH value in 5. 2-5. 5,5. 6-5. 8 and 5. 9-6. 8. The results showed that red blood cell (RBC), haemoglobin (Hb), packed cell volume(PCV)significant-ly increased with the reducing pH value, the number of white blood celKWBOwas increased. The leukocyte differential count was changed in the different degree, the proportion of eosinophilic leukocyte and lymphocyte was decreased,neutrophilic stab and segmented granulocyte was increased, mononuclear leucocyte was increased slightly. The pO2 was significantly increased. The pCO2 and HCO3- were significantly decreased. The levels of Ca2+ and Cl- were significantly decreased, the levels of Na+ and urea nitrogen were significantly increased,and there was no significant difference in the levels of K+. The decreased pCO2 and HCO3- in the slight and moderate

  17. 针刺对运动性疲劳大鼠部分生化指标的影响%Influence of Acupuncture on Biochemical Parameters in Exercise-induced Fatigue Rats

    Institute of Scientific and Technical Information of China (English)

    李虹霖; 孙远征; 丰晓溟

    2012-01-01

    目的:观察针刺对运动性疲劳大鼠部分生化指标的影响,探讨针刺抗运动性疲劳的作用机制.方法:采用4星期递增游泳训练的方法复制慢性运动性疲劳大鼠模型,随机分为对照组、模型组、头穴组、体穴组,采用“逆针灸”的方法.观察治疗前后各组大鼠力竭游泳时间、全血乳酸(Lactic Acid,LA)、肌酸激酶(Creatine Kinase,CK)、血尿素氮(Blood Urea Nitrogen,BUN)、血红蛋白(Hemoglobin,Hb)及游离色氨酸/支链氨基酸(free Tryptophan/Branched Chain Amino Acid,F-TRP/BCAA)的变化.结果:与体穴组相比,头穴组在提高Hb水平,降低BUN、LA水平方面有明显优势(P<0.05).在降低CK、F-TRP/BCAA水平方面,更是明显优于体穴组(p<0.01).结论:头穴针刺能够有效改善运动性疲劳大鼠生化指标,同时降低F-TRP/BCAA水平,抑制5-HT生成,从而有效地改善大鼠疲劳程度.%Objective:To observe the effect of acupuncture on some biochemical parameters in rats with exercise-induced fatigue and to explore the mechanism of acupuncture for exercise fatigue.Methods:The rat model of chronic exercise-induced fatigue was established by a 4-week increasing swimming training method.The rats were randomly divided into a control group,a model group,a scalp acupoint group,and a body acupoint group.Preventive acupuncture methods were used.Changes of exhaustive swimming time,whole blood lactic acid (LA),creatine kinase (CK),blood urea nitrogen (BUN),hemoglobin (Hb) and free tryptophan/branched chain amino acid (F-TRP/BCAA) of rats in each group before and after treatments were observed.Results:Compared with the body acupoint group,there were obvious advantages of the scalp point group in increasing Hb levels and lowering BUN and LA levels (P<0.05),as well as in aspects of lowering CK and F-TRP/BCAA levels (P<0.01).Conclusion:Scalp point acupuncture can improve the biochemical parameters in rats with exercise-induced fatigue,meanwhile,decrease the F

  18. Ⅰ型、Ⅱ型酮病对奶牛血液某些理化参数的影响%Effect of typeⅠand type Ⅱ ketosis on Some Blood Biochemical Parameters of Dairy Cow

    Institute of Scientific and Technical Information of China (English)

    李影; 孙玲伟; 张洪友; 夏成; 徐闯; 高阳; 钱伟东

    2014-01-01

    本试验旨在了解Ⅰ型、Ⅱ型酮病发生时奶牛体内某些理化参数的变化。在某集约化牛场随机选取产后7~28 d、平均胎次为2~3胎的试验奶牛,根据血浆中β-羟丁酸(BHBA)、葡萄糖(Glc)、游离脂肪酸(NEFA)的含量以及临床发病特点分为Ⅰ型酮病组(20头)、Ⅱ型酮病组(20头)和健康对照组(10头)。结果显示,三组间血浆中Glc、BH-BA、NEFA的浓度与体况评分存在显著差异(P<0.05),但三组试验奶牛的泌乳量、产后天数、血浆中胰岛素(INS)含量彼此间无显著性差异(P>0.05)。Ⅰ型酮病、Ⅱ型酮病与健康对照组之间的理化参数存在显著差异,为深入探究奶牛Ⅰ型酮病与Ⅱ型酮临床病理学、早期检测和诊断以及详细的发病机理提供依据,为今后有效地预防奶牛Ⅰ型、Ⅱ型酮病奠定基础。%The objective is to investigate some biochemical parameters changes in type I and typeⅡ ketosis of dairy cows. The experimental cows with two or three parities were selected at 7~28 days postpartum in a intensive dairy farm. Cows were classified as type I ketotic (K1, 20 cows), type Ⅱ ketotic (K2, 20 cows), and healthy control group (C, 10 cows) based on the glucose (Glc) and 3-hydroxybutyrate acid (BHBA) levels and the clinical signs. The results revealed that body condition scores, Glc, BHBA and NEFA plasma levels were significantly different among three groups (P0.05). Conclusion that some biochemical parameters were significantly different among type I ketosis, type Ⅱketosis and healthy control cows, thereby providing impor-tant information on the pathogenesis, early diagnosis, and prevention of type I and type Ⅱ keto-sis in dairy cows.

  19. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    Science.gov (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver. PMID:25837382

  20. Molecular and biochemical studies on bovine ephemeral fever

    Directory of Open Access Journals (Sweden)

    Nahed S. Thabet; Emad W. Ghazy; Mohamed A. Nayel; Mohamed Abo-Elkhair

    2011-05-01

    Full Text Available Bovine ephemeral fever (BEF in cattle has been reported to be associated with a range of biochemical changes which are similar to those seen in milk fever. This study aimed to clarify the biochemical alterations that associate infection of cattle with BEF with special references to the mechanisms involved in the development of hypocalcemia. The study was conducted on 30 cases of cattle infected with BEF based on the characteristic clinical signs which were confirmed by isolation of virus and RT-PCR. Another 6 healthy cows were used in the study as control. The evaluated parameters included biochemical variables such as serum values of total protein (TP, albumin (Alb, glucose (Glu, total calcium (tCa, ionized calcium (iCa, inorganic phosphorus (P, magnesium (Mg, sodium (Na, potassium (K, chloride (Cl, creatinine (Cr, blood urea nitrogen (BUN and serum activity of alkaline phosphatase (ALP. Hormonal profile included parathyroid hormone (PTH, insulin (Ins, and cortisol (Cor. The results showed that BEF-infected animals demonstrated a significant decrease (P<0.05 in serum concentrations of TP, Glo, iCa, P, Na, K, BUN and ALP while the mean values of serum levels of Glu and Cl were significantly increased (P<0.05. The mean values of serum levels of PTH were significantly decreased (P<0.05 while serum concentrations of Ins and Cor showed a significant increase. It was concluded that the clinical signs of bovine ephemeral fever are related to the hypocalcemia resulting from suppression of parathyroid hormone which seems to be mediated by respiratory alkalosis caused by the disease. This explanation needs future studies to provide a direct link between measurement of blood indicators of acid-base status, blood biochemical parameters and urine analysis. However, this work can provide a good knowledge about the pathogenesis of the disease that can lead to better management and proper treatment.

  1. Biochemical reasoning for radiation protection and screening methods for radiation sensitivity and potential carcinogenicity

    International Nuclear Information System (INIS)

    Cells of different genetic characteristics respond differently to agents that modify radiation effects. When the modification is a result of chemical repair, reduction of the amount of damage by radical scavenging, production of hypoxia, or any other such mechanism, then the modification of the response will be the same for all types of cells, but not the same when biological or biochemical parameters are involved, because the differences between the cells affect the final outcome, and the genetic traits obviously become affected by chemical modifying agents. Some of these agents directly affect the repair of deoxyribonucleic acid (DNA) by mechanisms not yet understood. Another agent nicotinamide (NA), is directly linked to a repair pathway. Thus, a system that uses NA as a precursor of nicotinamide adenine dinucleotide (NAD)+, and uses NAD+ to produce the polymer polyadenosine diphosphate ribose (PADPR) appears to be an interesting and important factor in the biochemical events that may be linked to improved radioprotection. (author). 36 refs., 5 figs

  2. A case study of single hygroscopicity parameter and its link to the functional groups and phase transition for urban aerosols in Taipei City

    Science.gov (United States)

    Hung, Hui-Ming; Hsu, Chia-Hung; Lin, Wei-Ting; Chen, Yu-Quan

    2016-05-01

    The hygroscopicity, functional groups and phase transitions of urban aerosol particles in Taipei City were studied using a cloud condensation nuclei counter (CCNc) with a scanning mobility particle sizer (SMPS) and an attenuated total reflectance with infrared (ATR-IR) detection technique. With the assumption of larger particles being activated first, the derived single hygroscopicity parameter (κ) exhibited an increasing trend with particle size, i.e., from 0.022 ± 0.01 at 87 ± 10 nm to 0.13 ± 0.03 at 240 ± 20 nm. The collected size-selected particles were characterized using ATR-IR for the functional groups of alkyl, carbonyl, ammonium, sulfate and nitrate, which showed various size dependence patterns, linked to different formation mechanisms. The hygroscopic response based on the ratio (xW_solute) for sample film of absorption by the enhanced water-stretching peak to that by the selected solute showed a better consistency with pure ammonium sulfate for sub-micron size particles. Based on the derived ammonium sulfate volume fraction from IR analysis, the κ received from CCNc measurements was concluded mainly contributed by ammonium sulfate for sub-micrometer particles. The increasing trend of sodium nitrate absorbance at aerosol diameter ≥1 μm was due to a reaction of nitric acid with sea salt particles. The micrometer sized particles were apparent not only in a significantly higher xW_solute than pure sodium nitrate but also had a deliquescence RH of 69 ± 1%, similar to that of sodium nitrate-sodium chloride mixtures. Overall, the organic species in this study exhibited a low hygroscopicity with less than 0.036 of contribution for the overall κ, and the major hygroscopic material of urban aerosols consisted primarily of ammonium sulfate in the sub-micrometer particles and sodium nitrate with sea salt in the coarse particles.

  3. Vitamin A Affects Growth Performance and Serum Biochemical Parameters of Geese%维生素A对鹅生长性能及血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    孙淑洁; 王宝维; 葛文华; 张名爱; 李文立

    2012-01-01

    This experiment was conducted to study the effects of vitamin A on growth performance, serum biochemical parameters and tissue vitamin A content of Qingnonghui geese. Two hundred Qingnonghui geese at 1 day of age were selected and randomly divided into 5 groups with 4 replicates in each group and 10 geese in each replicate. Five groups were supplemented with 0, 1 500, 3 000, 6 000 and 12 000 IU/kg vitamin A in the basal diets, respectively. The experiment lasted for twelve weeks. The results showed as follows; 1) supplemental vitamin A significantly increased daily weight gain and daily feed intake (P 0. 05). 2) At 4 weeks of age, supplemented 6 000 IU/kg vitamin A significantly decreased serum uric acid content (P 0. 05) ; at 12 weeks of age, supplemented 6 000 IU/kg vitamin A significantly decreased the contents of serum blood glucose and uric acid (P 0. 05). 3) The serum and liver vitamin A content increased significantly with the increase of dietary vitamin A supplemental levels (P <0. 01). In conclusion, consideration from the growth performance, the optimal dietary levels of vitamin A are 8 000 IU/kg at weeks 1 to 4, and 9 000 IU/kg at weeks 5 to 12. While consideration from the serum biochemical parameters, the optimal dietary level of vitamin A is 6 000 IU/kg at weeks 1 to 4 and 5 to 12.%本试验旨在研究不同水平维生素A对青农灰鹅生长性能、血清生化指标及组织中维生素A含量的影响.选用1日龄青农灰鹅200只,随机分为5组,每组4个重复,每个重复10只鹅.各组在玉米-豆粕型基础饲粮中分别添加0、1 500、3 000、6 000和12 000 IU/kg的维生素A.试验期12周.结果表明:1)饲粮添加维生素A能显著提高1~4、5~12周龄青农灰鹅平均日增重和平均日采食量(P<0.05),降低死淘率,但对料重比影响不显著(P>0.05).2)4周龄时,添加6 000 IU/kg维生素A显著降低了血清尿酸含量(P<0.05),添加3 000和6 000 IU/kg维生素A极显著提高了

  4. RMBNToolbox: random models for biochemical networks

    Directory of Open Access Journals (Sweden)

    Niemi Jari

    2007-05-01

    Full Text Available Abstract Background There is an increasing interest to model biochemical and cell biological networks, as well as to the computational analysis of these models. The development of analysis methodologies and related software is rapid in the field. However, the number of available models is still relatively small and the model sizes remain limited. The lack of kinetic information is usually the limiting factor for the construction of detailed simulation models. Results We present a computational toolbox for generating random biochemical network models which mimic real biochemical networks. The toolbox is called Random Models for Biochemical Networks. The toolbox works in the Matlab environment, and it makes it possible to generate various network structures, stoichiometries, kinetic laws for reactions, and parameters therein. The generation can be based on statistical rules and distributions, and more detailed information of real biochemical networks can be used in situations where it is known. The toolbox can be easily extended. The resulting network models can be exported in the format of Systems Biology Markup Language. Conclusion While more information is accumulating on biochemical networks, random networks can be used as an intermediate step towards their better understanding. Random networks make it possible to study the effects of various network characteristics to the overall behavior of the network. Moreover, the construction of artificial network models provides the ground truth data needed in the validation of various computational methods in the fields of parameter estimation and data analysis.

  5. A case study of single hygroscopicity parameter and its link to the functional groups and phase transition for urban aerosols in Taipei City

    Science.gov (United States)

    Hung, Hui-Ming; Hsu, Chia-Hung; Lin, Wei-Ting; Chen, Yu-Quan

    2016-05-01

    The hygroscopicity, functional groups and phase transitions of urban aerosol particles in Taipei City were studied using a cloud condensation nuclei counter (CCNc) with a scanning mobility particle sizer (SMPS) and an attenuated total reflectance with infrared (ATR-IR) detection technique. With the assumption of larger particles being activated first, the derived single hygroscopicity parameter (κ) exhibited an increasing trend with particle size, i.e., from 0.022 ± 0.01 at 87 ± 10 nm to 0.13 ± 0.03 at 240 ± 20 nm. The collected size-selected particles were characterized using ATR-IR for the functional groups of alkyl, carbonyl, ammonium, sulfate and nitrate, which showed various size dependence patterns, linked to different formation mechanisms. The hygroscopic response based on the ratio (xW_solute) for sample film of absorption by the enhanced water-stretching peak to that by the selected solute showed a better consistency with pure ammonium sulfate for sub-micron size particles. Based on the derived ammonium sulfate volume fraction from IR analysis, the κ received from CCNc measurements was concluded mainly contributed by ammonium sulfate for sub-micrometer particles. The increasing trend of sodium nitrate absorbance at aerosol diameter ≥1 μm was due to a reaction of nitric acid with sea salt particles. The micrometer sized particles were apparent not only in a significantly higher xW_solute than pure sodium nitrate but also had a deliquescence RH of 69 ± 1%, similar to that of sodium nitrate-sodium chloride mixtures. Overall, the organic species in this study exhibited a low hygroscopicity with less than 0.036 of contribution for the overall κ, and the major hygroscopic material of urban aerosols consisted primarily of ammonium sulfate in the sub-micrometer particles and sodium nitrate with sea salt in the coarse particles.

  6. Recent abstracts in biochemical technology

    OpenAIRE

    R R Siva Kiran; Brijesh P

    2008-01-01

    “Recent abstracts in biochemical technology” is a collection of interesting research articles published in “List of biochemical technology journals” (Table 1). The abstracts are most likely to report significant results in biochemical technology.

  7. Indication of CPAP in Patients with Suspected Obstructive Sleep Apnea, Based on Clinical Parameters and a Novel Two-Channel Recording Device (ApneaLink): A Pilot Study

    OpenAIRE

    Carlos Alberto Nigro; Eduardo Dibur; Sofía Grandval; Facundo Nogueira

    2012-01-01

    Objective. To evaluate the accuracy and reliability of the medical decision based on the results of the hand scoring from a two-channel recording device (ApneaLink) plus clinical data for the prescription of a CPAP assay in patients with suspected OSA. Methods. 39 subjects were assessed in the sleep laboratory with polysomnography and ApneaLink. The patients completed the Epworth sleepiness scale and a clinical history. Two blinded independent observers decided to prescribe CPAP according to ...

  8. 不同属种肉类对大鼠血清生化指标与抗氧化能力的影响%Effects of different species meats on serum biochemical parameters and antioxidant status in rats

    Institute of Scientific and Technical Information of China (English)

    邓宇; 高峰; 周光宏

    2011-01-01

    探讨了不同属种肉类对大鼠血清生化指标和抗氧化机能的影响.试验选取体重约177 g的雄性SD大鼠90只,随机分为3组即狗肉组、羊肉组、鸭肉组,分别将日粮中的主要蛋白源替换为狗肉、羊肉、鸭肉(CP 31%).试验期为4周.结果表明:饲喂狗肉和羊肉能显著提高大鼠生长性能,且前者使得大鼠血清碱性磷酸酶(ALP)活性极显著升高(P<0.01);而饲喂鸭肉使得大鼠血清丙氨酸转移酶(ALT)活性显著升高(P<0.05),天冬氨酸转移酶(AST)、血清尿素氮(BUN)活性极显著升高(P<0.01).不同属种的肉之间,鸭肉组肝脏和肾脏中谷胱甘肽过氧化物酶(GSH-Px)活性均极显著升高(P<0.01).结果提示:饲喂狗肉和羊肉能促进机体蛋白质合成,有利于蛋白质沉积;饲喂鸭肉则具有增强机体抗氧化的能力.%The experiment was conducted to study the effect of different species meats on serum biochemical parameters and antioxidant status in rats. A total of 90 male SD rats with body weight of about 177 g were divided into dog meat group, mutton group and duck meat group in a randomized complete block design, and these three groups were respectively replaced the main dietary protein source with dog meat. mutton, duck meat ( CP% : 31%). The experimental stage was 4 weeks. The results were shown as follows : the growth performance of rats was markedly enhanced by feeding dog meat and mutton, and the activity of alkaline phosphatase ( ALP) of rats in dog meat group was enhanced significandy ( P<0. 01). But with the rats in duck meat group, the activity of alanine aminotransferase ( ALT) was increased significantly (P<0. 05) . the activity of aspartate aminotransferase ( AST) and serum urea nitrogen ( BUN) was enhanced significantly (P<0. 01). Among the groups of different species meats, the activity of glutathione peroxidase ( GSH) in liver and kidney of rats in duck meat group was significantly higher ( P<0. 01). The

  9. The effects of detergent, sodium tripoly-phosphate and ethoxyled oleyl-cetyl alcohol on metabolic parameters of the fungus Trichothecium roseum link

    Directory of Open Access Journals (Sweden)

    Stojanović Jelica

    2011-01-01

    Full Text Available The degradation of detergents that are dispersed in water and soil partially depends on the metabolic activities of fungi. Among the fungi that have this ability, Deuteromycetes are particularly noted for their biochemical characteristics. Taking this into account, it was of interest to analyze the influence of detergent and its main compounds, ethoxyled oleylcetyl alcohol (AOC and sodium tripoly-phosphate (TTP, on the metabolism of the fungus Trichothecium roseum. Our results revealed that both detergent and AOC had an inhibitory effect on the bioproduction of free organic acids, while TTP stimulated their production. Also, detergent inhibited the bioproduction of basic amino acids, with the exception of alanine. In addition, detergent applied at 1% concentration inhibited the bioproduction of proteins and the total biomass of the fungus, while AOC and TTP inhibited the production of proteins, but stimulatedl the production of Trichothecium.

  10. Restless legs syndrome in Parkinson's disease: clinical characteristics and biochemical correlations

    Directory of Open Access Journals (Sweden)

    Tiago Machado Guerreiro

    2010-12-01

    Full Text Available Restless legs syndrome (RLS is a neurological disorder that responds to dopaminergic drugs, indicating a common pathophysiology with Parkinson's disease (PD. The prevalence of RLS was estimated in a group of PD patients and its clinical and biochemical characteristics were analysed. Forty-eight patients with PD were evaluated into two groups, with and without RLS. Clinical characteristics assessed in both groups were age, gender, duration of PD, Hoehn and Yahr, and Schwab and England scales. Laboratory variables such as hemoglobin, s-iron, s-ferritin and creatinine were obtained. The prevalence of RLS was 18.75%. No significant differences regarding clinical variables and biochemical parameters were observed. The high prevalence of RLS found in PD patients suggests the concept of a common etiological link and it seems that secondary causes did not play a central role in the pathophysiology of RLS in this group of parkinsonian patients.

  11. Hematological and biochemical parameters of Dorper ewes
    Parâmetros hematológicos e bioquímicos de ovinos da raça Dorper

    OpenAIRE

    Karina Medici Madureira; Viviani Gomes; Brenda Barcelos; Breno Henrique Zani; Carolina de Lara Shecaira; Camila Ramos Costa; Fernando José Benesi

    2013-01-01

    In order to establish hematological and biochemical Dorper sheep values and evaluate the influence of sex and age in these determinations, 72 animals were assigned to three experimental groups according to age: Group 1 (G1) consisting of animals up to 12 months of age (n=28), Group 2 (G2) composed of animals from 13 to 36 months (n=37) and Group 3 (G3) for animals from 37 to 60 months (n=7). The animals were still divided in another two groups according to gender: Group 4 (G4) – females (n=57...

  12. Measures of Biochemical Sociology

    Science.gov (United States)

    Snell, Joel; Marsh, Mitchell

    2008-01-01

    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  13. Biochemical Education in Brazil.

    Science.gov (United States)

    Vella, F.

    1988-01-01

    Described are discussions held concerning the problems of biochemical education in Brazil at a meeting of the Sociedade Brazileira de Bioquimica in April 1988. Also discussed are other visits that were made to universities in Brazil. Three major recommendations to improve the state of biochemistry education in Brazil are presented. (CW)

  14. the Early Effects of Gamma radiation on the diurnal changes of Some Biochemical Parameters and the Role of α-lipoic acid as an Antioxidant in Male Albino rats

    International Nuclear Information System (INIS)

    In mammals most of the physiological and behavioral systems such as sleep-wake cycle, cardiovascular activity, endocrine system, blood pressure, body temperature and hepatic metabolism are regulated by the circadian clock. The aim of the present work was to investigate the disturbances induced after gamma irradiation on the metabolic diurnal rhythm and the role of α-alpha lipoic acid (ALA) in preventing/or decreasing these disorders in rat. Male Swiss albino rats were divided into 4 groups, one of which served as a normal control group, the second group was exposed to a single whole body gamma radiation dose of 6Gy, the third group was treated by gavage with ALA 100 mg/kg/day for 20 days and the fourth one was orally administered with ALA 100 mg/kg/day for 20 days before whole body gamma radiation at a dose of 6Gy. Rats were irradiated at 10 am and sacrificed at 11 am, 12 pm, 1 pm and 2 pm and then the biochemical analyses were performed. The results showed a significant increase in the level of lipid peroxidation products (MDA) at all time intervals. A significant increase was recorded also in the amount of free radicals at 11 am and 2 pm, and pyruvic acid level at 12 pm and 2 pm. A significant reduction of glutathione level (GSH) was recorded at 12 pm, 1 pm and 2 pm in the liver of the irradiated rats. Furthermore, a significant increase was registered in blood glucose level and blood aminotransferase activities (AST, ALT) in irradiated rats. Administration of ALA has significantly attenuated the radiation induced oxidative damage. It could be concluded that the biochemical changes induced by whole body gamma irradiation of rats are dependent on the circadian cycle. Further investigations are necessary to understand these complicated relations.

  15. Spiroergometric and Spirometric Parameters in Patients with Multiple Sclerosis: Are there any Links between these Parameters and Fatigue, Depression, Neurological Impairment, Disability, Handicap and Quality of Life in Multiple Sclerosis?

    Czech Academy of Sciences Publication Activity Database

    Řasová, K.; Brandejský, P.; Havrdová, E.; Zálišová, M.; Rexová, Patrícia

    2005-01-01

    Roč. 11, - (2005), s. 213-221. ISSN 1352-4585 Grant ostatní: GA UK(CZ) 1641/2002-IV-GA UK Institutional research plan: CEZ:AV0Z10300504 Keywords : fatigue * multiple sclerosis * spirometric and spiroergometric parameters Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 2.832, year: 2005

  16. Biochemical Neuroscience Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — This biochemistry lab is set up for protein analysis using Western blot, enzyme linked immunosorbent assays, immunohistochemistry, and bead-based immunoassays. The...

  17. Multiplexing oscillatory biochemical signals.

    Science.gov (United States)

    de Ronde, Wiet; ten Wolde, Pieter Rein

    2014-04-01

    In recent years it has been increasingly recognized that biochemical signals are not necessarily constant in time and that the temporal dynamics of a signal can be the information carrier. Moreover, it is now well established that the protein signaling network of living cells has a bow-tie structure and that components are often shared between different signaling pathways. Here we show by mathematical modeling that living cells can multiplex a constant and an oscillatory signal: they can transmit these two signals simultaneously through a common signaling pathway, and yet respond to them specifically and reliably. We find that information transmission is reduced not only by noise arising from the intrinsic stochasticity of biochemical reactions, but also by crosstalk between the different channels. Yet, under biologically relevant conditions more than 2 bits of information can be transmitted per channel, even when the two signals are transmitted simultaneously. These observations suggest that oscillatory signals are ideal for multiplexing signals. PMID:24685537

  18. The Correlation study on the production performance and blood biochemical parameters of Big bone chicken%大骨鸡血液生化指标与生产性能的相关性研究

    Institute of Scientific and Technical Information of China (English)

    陈宁; 郭丹; 门德才

    2014-01-01

    本研究利用3个血液酶活性标记对庄河大骨鸡的血液生化指标多态性进行研究,并与大骨鸡的产肉性能和产蛋性能进行相关性分析,筛选出用于大骨鸡产肉性能的优势遗传标记2个,产蛋性能的优势遗传标记1个,产蛋产肉兼用的优势遗传标记1个。%In this study, the polymorphism of blood biochemical indexes was investigated by the activities of three blood enzyme in the Zhuanghe bone chicken, and the correlation analysis be-tween meat and egg production performances were conducted in order to screen for two advantageous genetic markers of meat production and one advantageous genetic marker of egg production, one ad-vantageous genetic marker of both meat and egg production marker.

  19. Technetium-99m hexamethyl propylene amine oxime-labeled leukocyte scintigraphy at three different times in active ulcerative colitis. Comparison with colonoscopy and clinico-biochemical parameters in the assessment of disease extension and severity

    International Nuclear Information System (INIS)

    In this study, our objective was to define the usefulness of technetium-99m hexamethyl propylene amine oxime (Tc-99m HMPAO)-labeled leukocyte scintigraphy at three different time points in the assessment of disease extension and severity in patients with active ulcerative colitis (UC). Twenty-one consecutive patients (10 women, 11 men; mean age 42.4±12 years) with active UC were prospectively studied. All patients were diagnosed by colonoscopy and histopathology prior to inclusion. Scintigraphy was performed at 1 h, 2 h, and 4 h after Tc-99m HMPAO-labeled leukocyte injection. Clinic-biochemical activity score, total colonoscopic activity score, and total scintigraphic activity score at 1 h, 2 h, and 4 h were calculated for each patient. Sensitivity, specificity, and accuracy values of Tc-99m HMPAO-labeled leukocyte scintigraphy were calculated as follows, respectively: 1 h imaging 86%, 73%, and 83%; 2 h imaging 89%, 74%, and 86%; 4 h imaging 90%, 58%, and 83% in the detection of active inflammatory segments. Even though no statistically significant difference was found between 1 h, 2 h, and 4 h imaging with respect to the sensitivity, specificity of labeled leukocyte scintigraphy, the largest area under the curve value was found for 2 h imaging. Tc-99m HMPAO-labeled leukocyte scintigraphy has been found to be correlated well with colonoscopy in the assessment of both the extension and severity of UC. We recommend 2 h scintigraphic imaging because it provides the largest area under the curve value and decreases the number of false-positive results. (author)

  20. Using combined measurements of gas exchange and chlorophyll fluorescence to estimate parameters of a biochemical C3 photosynthesis model: a critical appraisal and a new integrated approach applied to leaves in a wheat (Triticum aestivum) canopy

    NARCIS (Netherlands)

    Yin, X.; Struik, P.C.; Romero, P.; Harbinson, J.; Evers, J.B.; Putten, van der P.E.L.; Vos, J.

    2009-01-01

    We appraised the literature and described an approach to estimate the parameters of the Farquhar, von Caemmerer and Berry model using measured CO2 assimilation rate (A) and photosystem II (PSII) electron transport efficiency (F2). The approach uses curve fitting to data of A and F2 at various levels

  1. Bioquímica plasmática de cascavéis (Caudisona durissa LINNAEUS, 1758 em cativeiro Blood biochemical parameters from captivity rattlesnake (Caudisona durissa LINNAEUS, 1758

    Directory of Open Access Journals (Sweden)

    Wellington Bandeira da Silva

    2010-12-01

    breeding stock, justifying the importance of studying these snakes' physiology. Blood biochemical analysis of 53 Caudisona durissa was performed using plasma samples in an automated equipment (Ciba Corning - Express Plus®. Mean values and its respective Standard deviations are: blood urea nitrogen (BUN -1.32mg dL-1 (±1.1; uric acid - 2.08mg dL-1 (±1.4; creatinine - 0.52mg dL-1 (±0.2; total protein - 3.7g dL-1 (±0.7; albumin - 1.62g dL-1 (±0.4; globulins - 2.08g dL-1 (±0.5; calcium - 15.25mg dL-1 (±2.8; phosphorus - 4.61mg dL-1 (±1.9; cholesterol - 171.58mg dL-1 (±52.7; triglycerides - 19.29mg dL-1 (±14.3; alkaline phosfatase - 31.04U L-1 (±12.4; aspartate aminotransferase (AST - 22.25U L-1 (±11.4; alanin aminotransferase (ALT 7.11U L-1 (±5.4; amylases - 1385.23U L-1 (±568.7. The following indexes were calculated: BUN/creatinine ratio - 2.5 and calcium/phosphorus ratio -3.3. Rattlesnake blood biochemical results above could be used as reference in clinical evaluation for captivity C. durissa.

  2. Biochemical Hypermedia: Galactose Metabolism.

    Directory of Open Access Journals (Sweden)

    J.K. Sugai

    2013-05-01

    Full Text Available Introduction: Animations of biochemical processes and virtual laboratory environments lead to true molecular simulations. The use of interactive software’s in education can improve cognitive capacity, better learning and, mainly, it makes information acquisition easier. Material and Methods: This work presents the development of a biochemical hypermedia to understanding of the galactose metabolism. It was developed with the help of concept maps, ISIS Draw, ADOBE Photoshop and FLASH MX Program. Results and Discussion: A step by step animation process shows the enzymatic reactions of galactose conversion to glucose-1-phosphate (to glycogen synthesis, glucose-6-phosphate (glycolysis intermediary, UDP-galactose (substrate to mucopolysaccharides synthesis and collagen’s glycosylation. There are navigation guide that allow scrolling the mouse over the names of the components of enzymatic reactions of via the metabolism of galactose. Thus, explanatory text box, chemical structures and animation of the actions of enzymes appear to navigator. Upon completion of the module, the user’s response to the proposed exercise can be checked immediately through text box with interactive content of the answer. Conclusion: This hypermedia was presented for undergraduate students (UFSC who revealed that it was extremely effective in promoting the understanding of the theme.

  3. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  4. Scandinavian links

    DEFF Research Database (Denmark)

    Matthiessen, Christian Wichmann; Knowles, Richard D.

    The European Round Table of Industrialists identified in the 1980ies 14 missing links in the transportation network of the continent. Three of them were found around the Danish island of Zealand. One link is within the nation, the other two are between nations. One link connects heavy economic ce...

  5. A coupled mechano-biochemical model for bone adaptation

    Czech Academy of Sciences Publication Activity Database

    Klika, Václav; Pérez, M. A.; García-Aznar, J. M.; Maršík, F.; Doblaré, M.

    2014-01-01

    Roč. 69, 6-7 (2014), s. 1383-1429. ISSN 0303-6812 Institutional support: RVO:61388998 Keywords : mechano-biochemical model * bone remodelling * BMU Subject RIV: BJ - Thermodynamics Impact factor: 1.846, year: 2014 http://link.springer.com/article/10.1007%2Fs00285-013-0736-9

  6. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C)

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2015-10-01

    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased (P diet as compared to those fed the basal diet. The serum content of cholesterol decreased and the thickness of egg shell increased in the hens fed the T2 and T3 diet compared to those fed the basal diet. Overall from the results of the present experiment, it can be concluded that diet supplementation with aqueous extract of T. terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition.

  7. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C)

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2016-06-01

    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased ( P < 0.001) in the hens fed the extract-included diet as compared to those fed the basal diet. The serum content of cholesterol decreased and the thickness of egg shell increased in the hens fed the T2 and T3 diet compared to those fed the basal diet. Overall from the results of the present experiment, it can be concluded that diet supplementation with aqueous extract of T. terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition.

  8. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C)

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2016-06-01

    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased ( P hens fed the extract-included diet as compared to those fed the basal diet. The serum content of cholesterol decreased and the thickness of egg shell increased in the hens fed the T2 and T3 diet compared to those fed the basal diet. Overall from the results of the present experiment, it can be concluded that diet supplementation with aqueous extract of T. terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition.

  9. Effects of adding aqueous extract of Tribulus terrestris to diet on productive performance, egg quality characteristics, and blood biochemical parameters of laying hens reared under low ambient temperature (6.8 ± 3 °C).

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2016-06-01

    A study was conducted using 144 laying hens to evaluate the effects of adding aqueous extract of Tribulus terrestris to diets on productive performance, egg quality traits, and some blood parameters of laying hens reared under cold stress condition (6.8 ± 3 °C). The birds were randomly assigned to each of four dietary treatments (C, T1, T2, and T3) with six replicate cages of six birds. Diet inclusion of aqueous extract of T. terrestris at the rate of 10, 20, and 30 ml/Lit offered to groups T1, T2, and T3, respectively, while group C served as the control diet with no addition. Feed intake (FI), feed conversion ratio (FCR), egg weight (EW), egg production (EP), and egg mass (EM) were evaluated during the 42-day trial period. The EP and EM increased, whereas FCR decreased (P hens fed the extract-included diet as compared to those fed the basal diet. The serum content of cholesterol decreased and the thickness of egg shell increased in the hens fed the T2 and T3 diet compared to those fed the basal diet. Overall from the results of the present experiment, it can be concluded that diet supplementation with aqueous extract of T. terrestris has beneficial effects on productive performance of laying hens reared under cold stress condition. PMID:26471188

  10. Advancement in biochemical assays in andrology

    Institute of Scientific and Technical Information of China (English)

    Wolf-BernhardSchill; RaftHenkel

    1999-01-01

    Determination of maikers of sperm function, accessory sex gland secretion and silent male genital tract inflammation is of considerable diagnostic value in the evaluation of male infertility. The introduction of biochemical tests into the analysis of male factor has the advantage that standardized assays with a coefficient of variafion characteristic of clinical chemistry are performed, in contrast to biological test systems with a large variability .Biochemical parameters may be used in clinical practice to evaluate the sperm fertitizing capacity (acrosin, aniline blue,ROS), to characterize male accessory sex gland secretinns (fructose, a-glucosidase, PSA), and to identify men with silent genital tract inflammation (elastase, C'3 complement component, coeruloplasmin, IgA, IgG, ROS). (As/an J Androl 1999 Jun; 1: 45-51)

  11. Students' Ability to Organize Biochemical and Biochemistry-Related Terms Correlates with Their Performance in a Biochemical Examination

    Science.gov (United States)

    Nagata, Ryoichi

    2007-01-01

    Organization is believed to be related to understanding and memory. Whether this belief was applicable in biochemical education was examined about two years after students had experienced biochemistry classes in their first year. The ability of organizing information in biochemistry was judged from the number of correct links of 886 biochemical…

  12. Kombucha tea fermentation: Microbial and biochemical dynamics.

    Science.gov (United States)

    Chakravorty, Somnath; Bhattacharya, Semantee; Chatzinotas, Antonis; Chakraborty, Writachit; Bhattacharya, Debanjana; Gachhui, Ratan

    2016-03-01

    Kombucha tea, a non-alcoholic beverage, is acquiring significant interest due to its claimed beneficial properties. The microbial community of Kombucha tea consists of bacteria and yeast which thrive in two mutually non-exclusive compartments: the soup or the beverage and the biofilm floating on it. The microbial community and the biochemical properties of the beverage have so far mostly been described in separate studies. This, however, may prevent understanding the causal links between the microbial communities and the beneficial properties of Kombucha tea. Moreover, an extensive study into the microbial and biochemical dynamics has also been missing. In this study, we thus explored the structure and dynamics of the microbial community along with the biochemical properties of Kombucha tea at different time points up to 21 days of fermentation. We hypothesized that several biochemical properties will change during the course of fermentation along with the shifts in the yeast and bacterial communities. The yeast community of the biofilm did not show much variation over time and was dominated by Candida sp. (73.5-83%). The soup however, showed a significant shift in dominance from Candida sp. to Lachancea sp. on the 7th day of fermentation. This is the first report showing Candida as the most dominating yeast genus during Kombucha fermentation. Komagateibacter was identified as the single largest bacterial genus present in both the biofilm and the soup (~50%). The bacterial diversity was higher in the soup than in the biofilm with a peak on the seventh day of fermentation. The biochemical properties changed with the progression of the fermentation, i.e., beneficial properties of the beverage such as the radical scavenging ability increased significantly with a maximum increase at day 7. We further observed a significantly higher D-saccharic acid-1,4-lactone content and caffeine degradation property compared to previously described Kombucha tea fermentations. Our

  13. Biochemical synthesis with stable isotopes

    International Nuclear Information System (INIS)

    Descriptions of the biochemical synthesis of glucose-13C6 from Agmenellum quadruplication; the biochemical labelling of [13C, 15N] Chlorella and [13C] E. coli, [15N] E. coli, and the production of lactic-13C3 acid utilizing Lactobacillus casei are discussed

  14. EVALUATING BIOCHEMICAL INTERNET RESOURCES

    Directory of Open Access Journals (Sweden)

    R.M. Lima

    2007-05-01

    Full Text Available Many people fail to properly evaluate INTERNET information. This is often due to alack of understanding of the issues, by responsible authorities, and, morespecifically, a lack of understanding of the structure and modis operandi of theINTERNET tool. The aim of this project was to analyze biochemical issuesavailable in WEB pages, evaluating contents quality, coverage, accuracy, authorityand currency. Twenty three sites were analyzed for their contents, presence ofbibliographical references, authorship, titles responsibility and adequacy to targetpublic. The great majority (95% did not mention bibliographic references andtarget public. Less than half divulged names and/or graduation status ofresponsibles. Some sites contained critical conceptual errors, such as: oxygen isessential for anaerobic respiration; presence of H2O in photosynthesis dark phase;yeast is a pluricellular fungal; the overall equation of photosynthesis with errors;NADH2 instead NAD+; etc. None of the analyzed sites was thus consideredexcellent. Although the use of the internet is expanding rapidly on collegecampuses, little is known about students usage; how they perceive the reality ofinternet information and how successful they are in searching through it. Our datastrenghthen the need for rigorous evaluation concerning to educational research ofbiochemical themes on the WEB.

  15. Spectroscopic characterization of collagen cross-links in bone

    Science.gov (United States)

    Paschalis, E. P.; Verdelis, K.; Doty, S. B.; Boskey, A. L.; Mendelsohn, R.; Yamauchi, M.

    2001-01-01

    Collagen is the most abundant protein of the organic matrix in mineralizing tissues. One of its most critical properties is its cross-linking pattern. The intermolecular cross-linking provides the fibrillar matrices with mechanical properties such as tensile strength and viscoelasticity. In this study, Fourier transform infrared (FTIR) spectroscopy and FTIR imaging (FTIRI) analyses were performed in a series of biochemically characterized samples including purified collagen cross-linked peptides, demineralized bovine bone collagen from animals of different ages, collagen from vitamin B6-deficient chick homogenized bone and their age- and sex-matched controls, and histologically stained thin sections from normal human iliac crest biopsy specimens. One region of the FTIR spectrum of particular interest (the amide I spectral region) was resolved into its underlying components. Of these components, the relative percent area ratio of two subbands at approximately 1660 cm(-1) and approximately 1690 cm(-1) was related to collagen cross-links that are abundant in mineralized tissues (i.e., pyridinoline [Pyr] and dehydrodihydroxylysinonorleucine [deH-DHLNL]). This study shows that it is feasible to monitor Pyr and DHLNL collagen cross-links spatial distribution in mineralized tissues. The spectroscopic parameter established in this study may be used in FTIRI analyses, thus enabling the calculation of relative Pyr/DHLNL amounts in thin (approximately 5 microm) calcified tissue sections with a spatial resolution of approximately 7 microm.

  16. Evolución de parámetros bioquímicos nutricionales en pacientes de hemodiálisis durante un año de seguimiento Evolution of nutritional biochemical parameters in hemodialysis patients during a one-year follow-up period

    Directory of Open Access Journals (Sweden)

    M. Palomares Bayo

    2008-04-01

    Full Text Available La elevada supervivencia actual del paciente en hemodiálisis (52% hasta 5 años, ha hecho que se pongan de manifiesto complicaciones crónicas como la elevada prevalencia de malnutrición en los enfermos, así como la importancia de la situación nutricional en la morbi-mortalidad que presentan. La causa de desnutrición proteico-calórica es multifactorial, aunque procesos de inflamación crónica asociada a la técnica de diálisis cobran cada vez más relevancia. Se han evaluado las variaciones de distintos parámetros bioquímicos nutricionales (proteínas totales, albúmina plasmática, transferrina y colesterol total de 73 pacientes en hemodiálisis durante un año de seguimiento. La edad media de los pacientes era de 53,3 ± 18,69 años, con 43 varones y 30 mujeres. El tiempo en programa de hemodiálisis ha sido de 43 ± 33 meses, con una duración media de la sesión de 246± 24 minutos y dosis media de hemodiálisis administrada de 1,37 ± 0,27 (KT/V (Daurgidas 2ª generación. Se ha observado un descenso en todos los parámetros bioquímicos evaluados, con diferencias estadísticamente significativas: Proteínas totales (p Current high survival in hemodialysis patients (52% at 5 years have made the chronic manifestations to emerge such as the high hyponutrition prevalence of these patients, as well as the importance of the nutritional status in their morbimortality. The reason for protein-caloric hyponutrition is multifactorial, although chronic inflammatory conditions associated to the dialysis technique are becoming more and more relevant. The variations in several nutritional biochemical parameters (total proteins, plasma albumin, transferrin, and total cholesterol have been assessed in 73 hemodialysis patients for one year. The mean age of the patients was 53.3 ± 18.69 years (43 males and 30 females. The average on hemodialysis program was 43 ± 33 months, with a mean session duration of 246 ± 24 minutes, and mean hemodialysis

  17. Specialization of Biochemical Oxygen Demand for Surface Water and Wastewater

    OpenAIRE

    F.M. Nurul; Z.Z. Abdul Rahman; A.N. Norizan

    2011-01-01

    Pollution of rivers is attributed to point and non-point sources and marine pollution originates mainly from land-based sources. Therefore in order to control the quality of the water a few parameters have been chosen as the index for determining the water pollution. Amongst the parameters, Biochemical Oxygen Demand, (BOD) is one of the most important and frequently used parameters for estimating the level of water pollution. BOD measures the amount of oxygen consumed by microorganism to util...

  18. A case study of evolutionary computation of biochemical adaptation

    International Nuclear Information System (INIS)

    Simulations of evolution have a long history, but their relation to biology is questioned because of the perceived contingency of evolution. Here we provide an example of a biological process, adaptation, where simulations are argued to approach closer to biology. Adaptation is a common feature of sensory systems, and a plausible component of other biochemical networks because it rescales upstream signals to facilitate downstream processing. We create random gene networks numerically, by linking genes with interactions that model transcription, phosphorylation and protein–protein association. We define a fitness function for adaptation in terms of two functional metrics, and show that any reasonable combination of them will yield the same adaptive networks after repeated rounds of mutation and selection. Convergence to these networks is driven by positive selection and thus fast. There is always a path in parameter space of continuously improving fitness that leads to perfect adaptation, implying that the actual mutation rates we use in the simulation do not bias the results. Our results imply a kinetic view of evolution, i.e., it favors gene networks that can be learned quickly from the random examples supplied by mutation. This formulation allows for deductive predictions of the networks realized in nature

  19. Enzyme and biochemical producing fungi

    DEFF Research Database (Denmark)

    Lübeck, Peter Stephensen; Lübeck, Mette; Nilsson, Lena;

    2010-01-01

    factories for sustainable production of important molecules. For developing fungi into efficient cell factories, the project includes identification of important factors that control the flux through the pathways using metabolic flux analysis and metabolic engineering of biochemical pathways....

  20. Effects of ω-3 Polyunsaturated Fatty Acid on Changes of Blood Biochemical Parameters in the Patients with Systemic Inflammatory Response Syndrome Caused by Multiple Trauma%ω-3多不饱和脂肪酸对多发伤SIRS患者血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    赵剑波; 张秀梅; 祝捷; 周红珠

    2013-01-01

    Objective explore the effects of ω-3 polyunsaturated fatty acid(ω-3 PUFAs) on blood biochemical parameters in the patients with systemic inflammatory response syndrome caused by multiple trauma.Methods sixty-seven cases of systemic inflammatory response syndrome patients caused by multiple trauma admitted from January 2010 to March 2012 in our hospital were randomly selected.67 patients were divided into two groups,namely,ω-3 PUFAs group (n =34) and placebo group (n =33).Blood biochemical parameters before the treatment and after the that were compared.Results After the treatment for 7 days,the temperature,pulse,respiratory rate,WBC,Glu,PaO2,AG and PaCO2 were significantly different compared with the values before treatment in ω-3 PUFAs group (P <0.05),HCO3 (P <0.O1),while Hb had no change.Compared with control group,the temperature,pulse,respiratory rate,WBC and PaO2 of ω-3 PUFAs group were also significantly changed (P < 0.05).Conclusion ω-3 PUFAs can regulate blood biochemical indicators in the patients with systemic inflammatory response syndrome and effectively control the systemic inflammatory response syndrome via improving the incidence rate of hypoxemia,metabolic acidosis,respiratory acidosis and thus relieving disease progression and reducing mortality.%目的 探讨ω-3多不饱和脂肪酸对多发伤引起的全身炎症反应综合征(SIRS)患者血液生化指标的影响.方法 从2010年1月~ 2012年3月笔者医院收治的多发伤引起的SIRS患者中采用数字表法随机抽取67例,并随机分为ω-3多不饱和脂肪酸治疗组(n=34)和安慰剂对照组(n=33),比较两组患者在治疗前后血液生化指标的变化情况.结果 治疗7天后,与治疗前相比,除Hb之外,治疗组患者的体温、心率、呼吸频率、WBC、Glu、PaO2、AG和PaCO2等各项指标均有显著性变化(P<0.05),HCO3-有极显著性差异(P<0.01);而与对照组相比,治疗组在体温、心率、呼吸频率、WBC和PaO2等方

  1. Evolution of Biochemical Parameters During Composting of Various Wastes Compost

    Directory of Open Access Journals (Sweden)

    N. Saidi

    2008-01-01

    Full Text Available In Tunisia the most treatment waste is landfill (50% of wastes were land filled and only 5% are composted. And since our soil become more and more poor in organic matter, green waste can be a significant source of organic matter; in parallel we cited the domestic waste and dead posodonia collected from beaches. All these wastes coming from various origins can be exploited to produce stable compost able to correct the deficiency of soil. Exploiting waste could lead at different quality of mature compost. We are not interested in only the quality of the mature compost but we are interested in the time of the composting cycle. The goals of this study were to characterize the maturity and the sanitary quality of compost in relation with the feed stock source (green waste (C1, green waste mixed with Posidonia (C2 and municipal solid waste (C3. The results obtained showed that the duration of the cycle of composting depends on the nature of the substrate. The longest cycle (200 days was observed with the feed stock source C3. The C/N ratios ranged between 22 and 27 at the beginning of the cycle of composting and decreased notably during time of composting. NH4-N decreased over the progress cycle and at the end of composting progress, all wastes presented a content of NH4-N not exceeding the maximal value recommended for mature compost (400 mg kg-1. The CO2 released by C1 was of approximately 6000 mg C-CO2 DM kg-1 at the start of the cycle and it reached at the end of the cycle of composting 2300 mg C-CO2 DM kg-1. Nevertheless, the deshydrogenase activity (DHA recorded was important during the thermophilous phase at the level of the three piles C1, C2 and C3, where it reached the respective values of 5.9; 6.2 and 4 TPFS/TPF/g of DM. Maturity stage showed the values of 0.3; 0.8 and 0.4 TPFS/TPF/g of DM, respectively. Salmonella appeared only at the level of the piles C2 et C3 at the beginning of composting. After 40th days composting these bacteria are not detected. Staphylocoques were not detected at the level of the two piles C1 and C2. The number of these bacteria was important in the compost C3, where it fluctuated between 103 and 105 bacteria g-1 of dry matter. Statistical analyses showed that the compost of municipal solid waste C3 presented a value of salinity (6.8 g Kg-1 of DM higher than those obtained at the level of the other studied piles 2.6 g kg%/1 of DM for C1; 4 g Kg-1 of DM for C2. We also noted that the compost C2 was relatively rich in P (2.17%/ of DM and MgO (2.62% of DM as compared with the two other studied piles which contain a percentage of MgO of 0. 73 in C1 pile and 0. 8 in C3 pile. Although important heavy metals contents determined in the three studied composts were lower than the levels indicated by the standards of the European Union.

  2. Variations of biochemical and hematic parameters following Taoist qigong practice

    OpenAIRE

    Manzaneque JM; Vera FM; Rodriguez FM; Vadillo M; Bendayan, R; Blanca Mena, María José; Heiniger, AI

    2014-01-01

    Qigong is an ancient Chinese psychosomatic discipline which employs specially designed body movements to achieve mind-body integration, preserve health, and pursue longevity. The Taoist school of qigong, one of the main traditions within this Chinese discipline, has a particular approach that emphasizes naturalness for the achievement of those goals. Albeit diverse methods of qigong have already been shown to display significant psychobiological effects, Taoist qigong has been scarcely invest...

  3. Effect Of Supplementation On Haematological And Biochemical Parameters During Pregnancy

    Directory of Open Access Journals (Sweden)

    Mohapatra P

    1988-01-01

    Full Text Available One hundred forty eight antenatal women were studied as supplemented (70 and non-supplemented (78 groups. The former group received nutritional supplementation in addition, to Iron, Folic acid and calcium tablets, which were given to both the groups. There was significant increase in the serum protein (p<0.001 and albumin (p<0.001 levels of the supplemented women. Haemoglobin was found to be increased in both the groups because of Iron therapy. But the increment was significantly higher (p<0.001 in supplemented group. Thus apart from Iron and Folic Acid, a diet rich in proteins (11 gm with calories (330 kcal is essential during pregnancy, at least for a period of 100 days.

  4. Ouroboros - Playing A Biochemical

    Directory of Open Access Journals (Sweden)

    D. T. Rodrigues

    2014-08-01

    Full Text Available Ouroboros: Playing A Biochemical RODRIGUES,D.T.1,2;GAYER, M.C.1,2; ESCOTO, D.F.1; DENARDIN, E.L.G.2, ROEHRS, R.1,2 1Interdisciplinary Research Group on Teaching Practice, Graduate Program in Biochemistry, Unipampa, RS, Brazil 2Laboratory of Physicochemical Studies and Natural Products, Post Graduate Program in Biochemistry, Unipampa, RS, Brazil Introduction: Currently, teachers seek different alternatives to enhance the teaching-learning process. Innovative teaching methodologies are increasingly common tools in educational routine. The use of games, electronic or conventional, is an effective tool to assist in learning and also to raise the social interaction between students. Objective: In this sense our work aims to evaluate the card game and "Ouroboros" board as a teaching and learning tool in biochemistry for a graduating class in Natural Sciences. Materials and methods: The class gathered 22 students of BSc in Natural Sciences. Each letter contained a question across the board that was drawn to a group to answer within the allotted time. The questions related concepts of metabolism, organic and inorganic chemical reactions, bioenergetics, etc.. Before the game application, students underwent a pre-test with four issues involving the content that was being developed. Soon after, the game was applied. Then again questions were asked. Data analysis was performed from the ratio of the number of correct pre-test and post-test answers. Results and discussion: In the pre-test 18.1% of the students knew all issues, 18.1% got 3 correct answers, 40.9% answered only 2 questions correctly and 22.7% did not hit any. In post-test 45.4% answered all the questions right, 31.8% got 3 questions and 22.7% got 2 correct answers. The results show a significant improvement of the students about the field of content taught through the game. Conclusion: Generally, traditional approaches of chemistry and biochemistry are abstract and complex. Thus, through games

  5. NOD/SCID 小鼠主要脏器重量、血生理生化指标和免疫细胞的测定%The study on organ weights, blood physiological and biochemical parameters and immune cells in NOD/SCID mice

    Institute of Scientific and Technical Information of China (English)

    王怀远; 吴旭颖; 张文明; 田玉书; 吴金峰; 王宇; 张亚静; 杨志伟

    2014-01-01

    目的:测定NOD/SCID小鼠的主要脏器重量、血生理生化指标和免疫细胞比例。方法选取5周龄和10周龄的NOD/SCID小鼠,测定主要脏器重量和血液生理生化指标;选取6周龄的NOD/SCID小鼠,利用流式细胞仪检测其T细胞及其亚群( T细胞-CD3+、T细胞亚群-CD4+T细胞、CD8+T细胞)、B细胞( CD19+或B220+)、NK细胞(NK1.1+)和粒细胞(CD11b+)。结果同性别NOD/SCID小鼠,不同周龄的双肾、肝脏、心脏和肺脏重量,血生理指标RBC、HGB、HCT、MCV、MCH和RDW,血生化指标TP、ALB、ALP、CHO和TBIL具有显著差异;同周龄的雌雄相比,血生理指标HCT,血生化指标GLOB、A/G、CHO、TG、TBIL和UN有显著差异。 NOD/SCID小鼠无T细胞(0.37±0.26)%、CD4+T细胞(0.35±0.13)%、CD8+T细胞(0.47±0.10)%、CD19+B细胞(0.13±0.05)%、B220+B细胞(1.20±0.44)%),有低水平NK细胞(6.90±0.82)%,粒细胞比例为(47.88±15.54)%。结论 NOD/SCID小鼠表现为为T、B、NK细胞联合免疫功能缺陷,周龄和性别对其脏器重量、血生理生化指标有一定影响。本研究的NOD/SCID小鼠品系的生理生化指标与国外生产的相同品系基本一致。%Objective To measure the organ weights , physiological and biochemical parameters and immune cells of NOD/SCID mice.Methods Mice at five and ten weeks of age were selected .The organ weights, blood physiological and biochemical parameters were observed .The percentages of CD 3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in NOD/SCID mice at six week of age in terms of its T , B lymphocyte function and NK cell activity.Results Among the same sex group of NOD/SCID mice, the weights of kidney, liver, heart and lung, and the blood physiological indexes of RBC , HGB, HCT, MCV, MCH, RDW, and the blood biochemical indexes of TP , ALB, ALP, CHO, TBIL show significant difference between 5 and 10

  6. Assessment of acute pesticide toxicity with selected biochemical variables in suicide attempting subjects

    International Nuclear Information System (INIS)

    Pesticide induced changes were assessed in thirty two subjects of attempted suicide cases. Among all, the farmers and their families were recorded as most frequently suicide attempting. The values obtained from seven biochemical variables of 29 years old (average age) hospitalized subjects were compared to the same number and age matched normal volunteers. The results revealed major differences in the mean values of the selected parameters. The mean difference calculate; alkaline phosphatase (178.7 mu/l), Bilirubin (7.5 mg/dl), GPT (59.2 mu/l) and glucose (38.6 mg/dl) were higher than the controls, which indicate the hepatotoxicity induced by the pesticides in suicide attempting individuals. Increase in serum creatinine and urea indicated renal malfunction that could be linked with pesticide induced nephrotoxicity among them. (author)

  7. Efficacy of topical honey therapy against silver sulphadiazine treatment in burns: A biochemical study

    OpenAIRE

    Nagane, N. S.; Ganu, J. V.; Bhagwat, V. R.; Subramanium, M.

    2004-01-01

    Thermal injury is associated with biochemical changes. The present study was undertaken to investigate relation of oxidative free radical generation and related biochemical parameters in burn trauma. The specific aim was to compare the levels of serum lipid peroxide, Ceruloplasmin and Uric Acid in burn patients during treatment with Silver Sulfadiazine Cream and honey therapy. It is a single blind prospective controlled study involving comparison of biochemical changes after treatment with si...

  8. Link Analysis

    Science.gov (United States)

    Donoho, Steve

    Link analysis is a collection of techniques that operate on data that can be represented as nodes and links. This chapter surveys a variety of techniques including subgraph matching, finding cliques and K-plexes, maximizing spread of influence, visualization, finding hubs and authorities, and combining with traditional techniques (classification, clustering, etc). It also surveys applications including social network analysis, viral marketing, Internet search, fraud detection, and crime prevention.

  9. Blood biochemical parameters, nitrogen balance and gross energy metabolizability in pigs fed with different dietary electrolyte balancesParâmetros bioquímicos sanguíneos, balanço de nitrogênio e metabolizabilidade da energia bruta em suínos alimentados com dietas contendo diferentes balanços eletrolíticos

    Directory of Open Access Journals (Sweden)

    Luís Daniel Giusti Bruno

    2012-08-01

    Full Text Available The aim of this study was to evaluate different dietary electrolyte balance (DEB on the blood biochemical parameters, nitrogen balances and crude metabolizable energy of swine in the initial phase. Sixteen barrows were used, averaging 27.95 kg of initial weight, allotted individually in metabolic cages in a randomized blocks design with four treatments (DEB of 160, 208, 257 and 305 mEq/kg and 4 replicates. The blood biochemical parameters analyzed were chloride, potassium, sodium and urea in blood. Urine pH was also evaluated, and the parameters evaluated in nitrogen (N balance were N intake, fecal N, N excreted in urine, absorbed N, N retention, retained N/absorbed N and total excretion of N. The gross energy digestibility and metabolizibility coefficients were determined, and the respectives values of digestible and metabolizable energy. The values of urinary pH increased (P 0.05 between the evaluated DEB. A linear increase (P Objetivou-se com esse trabalho avaliar diferentes balanços eletrolíticos das dietas (BED sobre os parâmetros bioquímicos sanguíneos, balanços de nitrogênio e a metabolizabilidade da energia bruta, de suínos na fase inicial. Foram utilizados 16 suínos, machos castrados, com peso inicial de 27,95 kg, distribuídos individualmente em gaiolas metabólicas, em um delineamento experimental em blocos ao acaso com quatro tratamentos (BED de 160, 208, 257 e 305 mEq/kg e quatro repetições. Os parâmetros bioquímicos sanguíneos avaliados foram cloretos, potássio, sódio e uréia no soro. O pH da urina também foi avaliado, e as variáveis utilizadas no balanço de nitrogênio (N foram o N ingerido, N excretado nas fezes, N excretado na urina, N absorvido, N retido, N retido/N absorvido e excreção total de N. Foram determinados os coeficientes de digestibilidade e metabolizabilidade da energia bruta e seus respectivos valores de energia digestível e metabolizável. Os valores de pH urinário aumentaram (P 0,05 entre

  10. Operative links

    DEFF Research Database (Denmark)

    Wistoft, Karen

    2010-01-01

    as networks: second, a semantic perspective on discourses and concepts of health, and, third, a health pedagogical perspective on participation, intervention, and roles. This paper argues for the importance of 'operative links' between different levels in health strategies. It is proposed that such......The article combines a public management perspective with a pedagogical perspective on health promotion. Our aim is to look into recent reforms in Denmark on health promotion in order to see how managerial ideas have been combined with welfare professional ideals concerning broad, positive and...... links are supported by network structures, shared semantics and situated pedagogical means of intervention....

  11. Hematological and Biochemical Responses of the Flowerhorn Fish to Hypoxia

    Directory of Open Access Journals (Sweden)

    Pakanit Kupittayanant

    2011-01-01

    Full Text Available Hematological and biochemical responses of the flowerhorn fish (Amphilophus trimaculatus x Amphilophus citrinelllus x Vieja synspilum and their surviving strategies to hypoxia were investigated. Male and female flowerhorn fish were divided into five groups. Each group contained tree replications of five groups and was exposed to hypoxia by the substitution of nitrogen for oxygen for 12, 24 and 48 h, respectively. Blood sample was collected from the caudal vein and physiological-biochemical blood parameters analyzed. The results showed that hypoxia caused a significant increase in ventilation rate. Hematological parameters including red blood cell count, white blood cell count, hematocrit and hemoglobin concentration were significantly increased in fish exposed to hypoxia whereas mean corpuscular hemoglobin concentration remained the same. In addition, hypoxia caused significant increases in serum glucose, alanine amino-transferase, aspartate amino-transferase, creatine kinase and blood urea nitrogen. However, cholesterol and creatinine were significantly decreased. The effects of hypoxia on those parameters occurred in a time dependent manner. Changes in hematological parameters fully recovered after 1 week oxygen replenishment whereas biochemical parameters slowly returned to control levels. These suggest that acute hypoxia in the flowerhorn fish up to 48 h can affect physiology functions as indicated by changes in hematological and biological parameters. Alteration of most physiological functions can be restored by repayment of oxygen debt. The results also suggest that the flowerhorn fish coped with hypoxic condition by using both energy saving strategies and by attempting to increase the oxygen extraction capacity.

  12. "Conjectural" links in complex networks

    OpenAIRE

    Snarskii, A. A.; Zorinets, D. I.; Lande, D. V.

    2016-01-01

    This paper introduces the concept of Conjectural Link for Complex Networks, in particular, social networks. Conjectural Link we understand as an implicit link, not available in the network, but supposed to be present, based on the characteristics of its topology. It is possible, for example, when in the formal description of the network some connections are skipped due to errors, deliberately hidden or withdrawn (e.g. in the case of partial destruction of the network). Introduced a parameter ...

  13. Blood gas and blood biochemical parameter change on the correlation analysis of early diagnosis of pulmonary edema%血气及血生化参数改变对肺水肿早期诊断的相关分析

    Institute of Scientific and Technical Information of China (English)

    郭大敏; 劳程强; 曾灶英

    2015-01-01

    目的:探讨血气以及血生化参数的改变对肺水肿早期诊断的相关意义,为肺水肿的及时确诊和治疗提供一定的理论依据。方法从2013年2月~2014年7月我院收治的肺水肿患者中选取34例作为观察组,同期选择34例健康志愿者作为对照组,分别检测观察组以及对照组的血常规、血气以及血生化指标,并对两个实验组的各项指标进行比较分析。结果观察组的白细胞计数(WBC)明显高于对照组,差异具有统计学意义(P<0.05)。通过对比两个实验组的血气指标结果发现,观察组的pH值、PaO2、PaCO2、SaO2、以及FiO2/PaO2均显著低于对照组,差异具有统计学意义(P<0.05)。而观察组和对照组的血生化指标情况除在K+上存在较为明显差异之外,其余Na+、Cl-、BUN(尿素氮)以及GLU(血糖)差异均无统计学意义(P>0.05)。结论肺水肿患者在早期出现白细胞计数明显增加以及低氧血症持续性进行性加重,加强血常规及血气指标的检测,有利于肺水肿的早期诊断和治疗。%ObjectiveTo investigate the blood gas and blood biochemical parameters related to the change of early diagnosis of pulmonary edema, provides certain theory basis for timely diagnosis and treatment of pulmonary edema.MethodsPulmonary edema patients were randomly selected in our hospital from February 2013 to July 2014, 34 cases as the observation group, select the same period 34 cases of healthy volunteers as control group, the observation group and the control group were detected in the blood routine test, blood gas and blood biochemical index, and the indicators of the two experimental groups were compared and analyzed.Results The white blood cell count (WBC) in the observation group were significantly higher than the control group, the difference was statistically significant (P0.05).Conclusion Patients with pulmonary edema appeared white blood cell count

  14. Infecção experimental em suínos jovens com Leptospira interrogans sorovar wolffi: determinação de parâmetros bioquímicos Experimental infection by Leptospira interrogans serovar wolffi in young pigs: determination of biochemical parameters

    Directory of Open Access Journals (Sweden)

    José Carlos Rende

    2007-04-01

    were inoculated by the same way with 5.0mL of a cell culture containing 1.0x10(8cells/mL of Leptospira interrogans sorovar wolffi, wild strain L-10, isolated from a wild especies of brazilian "armadillo" (Dasypus novemcinctus. Three days after inoculation, blood was collected without anticoagulant; the same process was repeated at 72 hours intervals during eighteen days in both, control and experimental groups. Quantitative biochemical parameters were direct, indirect and total bilirubin, fatty acids and serum proteins. The experimental animals showed an increase of direct bilirubin three days after inoculation. Increases in indirect and total bilirubin were, also, observed after six days. Glucose, fatty acids and serum proteins decreased after the third day of inoculation. In conclusion, the increase in bilirubin levels could be due to acute hemolysis; hypoglycemia, hypolipidemia and hypoproteinemia that could be related to hepatic lesions and septicemia. All parameters returned to normal levels after fifteen days, in all animals tested.

  15. Dietary Fat Levels Affect Growth Performance, Body Fat Deposition and Serum Biochemical Parameters of Growing Blue Foxes (Alopex lagopus)%饲粮脂肪水平对育成期蓝狐生长性能、体脂沉积及血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    耿业业; 张铁涛; 张志强; 高秀华; 杨福合; 邢秀梅

    2011-01-01

    本试验旨在研究饲粮脂肪水平对蓝狐生长性能、体脂沉积及血清生化指标的影响.选取(55±5)日龄、体重相近的健康蓝狐64只,随机分成4组,每组8个重复,每个重复2只(公母各占1/2),分别饲喂脂肪水平为12%、26%、40%和54%的试验饲粮.预试期为10 d,正试期为156 d.结果表明,试验狐各阶段日增重随饲粮脂肪水平的增加呈现先升高再降低的趋势,而血脂、血糖及内脏脂肪率则呈逐渐升高的趋势.当脂肪水平达到54%时,试验狐的肝脂率和肝体指数均显著高于其他组(P<0.05).母狐血清生化指标与体脂沉积各性状的相关性比公狐高.血清甘油三酯和高密度脂蛋白胆固醇水平与肝脂率、肝体指数及内脏脂肪率呈显著正相关(P<0.05).经对蓝狐生长性能、体脂沉积、血清生化指标综合分析得出,蓝狐具有较高的脂肪耐受性,但育成期蓝狐饲粮脂肪水平不宜超过40%,公狐以饲粮脂肪水平26%组,母狐以饲粮脂肪水平40%组生长最快.%The trial was conducted to evaluate the effect of dietary fat levels on growth, body fat deposition, and serum biochemical parameters of growing blue foxes (Alopex lagopus). Based on age and initial body weight, a total of 64 blue foxes were randomly assigned into 4 groups with 8 replicates per group and 2 foxes per replicate (half male and half female). Foxes in four groups were fed with experimental diets containing 12% , 26% , 40% or 54% fat, respectively. The experiment included an adaptation period for 10 days and a test period for 156 days. The results showed as follows; with the increasing dietary fat levels, daily gain weight of growing blue foxes was firstly increased and then decreased, while the contents of fat and glucose in serum and visceral fat percentage (VFP) were improved at different stages. When the dietary fat level was 54% , hepatic fat percentage (HFP) and hepatic somatic index (HIS) were significantly

  16. 肉鸡高、低脂系体脂性状和血清生化指标的比较分析%Comparative analysis of body fat traits and serum biochemical parameters between broiler lines divergently selected for abdominal fat content

    Institute of Scientific and Technical Information of China (English)

    王海威; 王启贵; 王守志; 户国; 李辉

    2009-01-01

    以东北农业大学肉鸡高、低腹脂双向选择品系为试验材料,分析第11、12世代肉鸡体重、体脂性状和血清生化指标在两系间的差异.结果表明,在体重和体脂性状方面,肉鸡高脂系的腹脂重、腹脂率、肝脏重极显著高于低脂系肉鸡的相应性状(P0.05).在血清生化指标方面,高脂系肉鸡血清中HDL-C、VLDL、FFA、apoB和胰岛素(Insulin)水平高于低脂系肉鸡的相应指标(P0.05).上述结果表明,对肉鸡腹脂率的持续定向选择对血液中脂质代谢各项生化指标具有显著的影响.有助于理解肉鸡血脂代谢与体脂沉积的关系,对低脂肉鸡的进一步选育具有积极的指导意义.%The fat and lean line broilers in the 11th and 12th generation were studied, which were divergent selection by abdominal fat percentage and plasma VLDL concentration. The difference of body weight, fat traits and serum biochemical parameters were analyzed in the two lines. The results showed that the difference of body weight at 7 weeks of age was not significant in two lines, and the difference of abdominal fat weight, abdominal fat percentage, liver weight, and liver fat percentage was very signifi-cant in two lines, and lean line were lower(P<0.01, P<0.05). However, the pectoralis intramuscular fat percentage, and leg intramuscular fat percentage in lean line were higher than those of fat line (P<0.01). The difference of TG and TC concentration in serum was not significant in two lines, and the difference of HDL-C, VLDL, FFA, Insulin and apoB concentration in serum was very significant in two lines, and lean line were lower (P<0.01). However, the Glu, and LDL-C concentration in serum in lean line were higher than those of fat line (P<0.01). So the current study could draw a conclusion that abdominal fat percentage of broilers continued directional selection have a significant impact on the blood biochemical parameters of lipid metabolism.

  17. Influencia de Especies Arbóreas Implantadas sobre Parámetros Biológicos y Bioquímicos en un Suelo Forestal de Chubut, Argentina Influence of Implanted tree Species on Biological and Biochemical Parameters in a Forest soil of Chubut, Argentina

    Directory of Open Access Journals (Sweden)

    Diana N Effron

    2012-01-01

    Full Text Available Se estudió la influencia de algunas especies forestales sobre propiedades biológicas y bioquímicas de un suelo, con el propósito de contribuir a establecer criterios que logren un desarrollo sustentable para un sistema forestal. El sitio de estudio es un suelo forestal de Chubut, en la Patagonia Argentina. Se tomaron muestras superficiales de suelo de tres parcelas con una especie dominante cada una: Roble (Quercus robur, Fresno (Fraxinus excelsior L. y Pino radiata (Pinus radiata D. Don.. En ellas se determinaron carbono de respiración, actividad deshidrogenasa, actividad fosfatasa ácida, número de bacterias totales y amilolíticas, actinomicetes y hongos totales, mientras que en las hojas se determinó fósforo. Los resultados mostraron diferencias importantes de estos parámetros para las diferentes especies estudiadas, diferencias que son explicadas por la composición química de las diferentes especies vegetales.The influence of some tree species on biological and biochemical properties of a forest soil was studied with the purpose of establishing criteria to achieve a sustainable development of a forest system. The study site is a forest soil of Chubut, in the Argentinean Patagonia. Surface soil samples were taken of three plots with a dominant species each one: Oak (Quercus robur, Ash (Fraxinus excelsior L. and Radiata Pine (Pinus radiata D. Don.. Microbial respiration, dehydrogenase and acid phosphatase activities, counts of aerobic and amylolytic bacteria, actinomyces and fungi were determined in soil samples, while phosphorous was determines in leaves. The results showed important differences of these parameters for the different species considered in the study, differences that are explained by the different chemical composition of the vegetal species.

  18. Robust simplifications of multiscale biochemical networks

    Directory of Open Access Journals (Sweden)

    Zinovyev Andrei

    2008-10-01

    Full Text Available Abstract Background Cellular processes such as metabolism, decision making in development and differentiation, signalling, etc., can be modeled as large networks of biochemical reactions. In order to understand the functioning of these systems, there is a strong need for general model reduction techniques allowing to simplify models without loosing their main properties. In systems biology we also need to compare models or to couple them as parts of larger models. In these situations reduction to a common level of complexity is needed. Results We propose a systematic treatment of model reduction of multiscale biochemical networks. First, we consider linear kinetic models, which appear as "pseudo-monomolecular" subsystems of multiscale nonlinear reaction networks. For such linear models, we propose a reduction algorithm which is based on a generalized theory of the limiting step that we have developed in 1. Second, for non-linear systems we develop an algorithm based on dominant solutions of quasi-stationarity equations. For oscillating systems, quasi-stationarity and averaging are combined to eliminate time scales much faster and much slower than the period of the oscillations. In all cases, we obtain robust simplifications and also identify the critical parameters of the model. The methods are demonstrated for simple examples and for a more complex model of NF-κB pathway. Conclusion Our approach allows critical parameter identification and produces hierarchies of models. Hierarchical modeling is important in "middle-out" approaches when there is need to zoom in and out several levels of complexity. Critical parameter identification is an important issue in systems biology with potential applications to biological control and therapeutics. Our approach also deals naturally with the presence of multiple time scales, which is a general property of systems biology models.

  19. Haematological and biochemical parameters in Churra-da-Terra-Quente ewes from the northeast of Portugal Parâmetros hematológicos e bioquímicos de ovelhas da raça Churra da Terra Quente do nordeste de Portugal

    Directory of Open Access Journals (Sweden)

    I.R. Dias

    2010-04-01

    Full Text Available Hematological and biochemical parameters, including plasma electrolytes and thyroid hormones, were determined in 73 clinically healthy Churra-da-Terra-Quente ewes, a typical breed from the northeast of Portugal. The hemogram values were: erythrocytes 9.8±1.5×10(12/L; haemoglobin 118.1±19.1g/L; haematocrit 40.8±5.9%; leukocytes 5.7±1.8×10(9/L; and platelets 544.3±177.2×10(9/L. The thrombin time was 17.3±1.7 seconds. The values of biochemical parameters were: total protein 76.4±6.1g/L; glucose 2.87±0.60mmol/L; total cholesterol 1.65±0.33mmol/L; aspartate aminotransferase 155.9±49.2U/L; alanine aminotransferase 23.2±9.6U/L; γ-glutamyl transferase 48.0±18.7U/L; total alkaline phosphatase 121.6±76.1U/L; glutamate dehydrogenase 6.4±3.7U/L; urea 7.32±2.22mmol/L; creatinine 123.0±54.1μmol/L; total calcium 2.53±0.25mmol/L; phosphorus 2.10±0.46mmol/L; magnesium 1.01±0.09mmol/L; sodium 152.04±3.65mmol/L; potassium 4.7±0.4mmol/L; ionized calcium 1.32±0.07mmol/L; total thyroxine 111.75±42.29nmol/L; total triiodothyronine 1.01±0.28nmol/L; free T4 11.93±1.78pmol/L; free T3 4.22±1.33pmol/L; and thyroid-stimulating hormone 0.18±0.19μIU/mL. Although differences among the Churra-da-Terra-Quente breed and other breeds may occur, the hematological and biochemical parameters, plasma electrolytes, and thyroid hormones, for this indigenous breed, were generally situated within the reference intervals previously reported for sheep.Os valores hematológicos e bioquímicos, incluindo os eletrólitos plasmáticos e os hormônios da tireoide, foram determinados em 73 ovelhas, clinicamente saudáveis, da raça Churra da Terra Quente, raça ovina característica do nordeste de Portugal. Os valores obtidos para o hemograma foram: eritrócitos 9,8±1,5×10(12 /L; hemoglobina 118,1±19,1g/L; hematócrito 40,8±5,9%; leucócitos 5,7±1,8×10(9 /L e plaquetas 544,3±177,2×10(9/L. O tempo de trombina foi de 17,3±1,7 segundos. Os valores dos par

  20. 复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响%Milk production,nitrogen excretion and blood biochemical parameter responses to di-etary addition of compound yeast cultures in dairy cows

    Institute of Scientific and Technical Information of China (English)

    王玲; 吕永艳; 程志伟; 杜高唐; 李金林; 付石军; 孙国强

    2015-01-01

    为研究饲料中添加复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响,选取年龄、体重、产奶量及泌乳期相近(135±15)d 的荷斯坦奶牛24头,随机分为4组,每个处理6个重复,对照组和3个试验组的复合酵母培养物添加量分别为精料浓度的0,0.8%,1.0%,1.2%,随精料饲喂,测定产奶量、乳成分、氮排放及血液生化指标,结果表明,1)试验2组日均产奶量显著高于对照组(P <0.05),各试验组分别比对照组提高8.48%,10.05%,8.97%。2)复合酵母培养物能显著提高乳脂和乳蛋白率(P <0.05),显著降低牛奶体细胞数(P <0.05),以试验2组最低。3)在氮排放量上,试验2组显著低于对照组(P <0.05),各试验组比对照组分别降低8.47%,12.01%,9.36%。4)在血液生化指标方面,复合酵母培养物能提高血清中总蛋白、球蛋白、血糖、胰岛素水平(P <0.05),降低尿素氮水平(P <0.05)。由此可见,本试验条件下,综合考虑产奶量、乳成分、氮排放及血液生化指标,复合酵母培养物的最佳添加量为精料浓度的1.0%。%This experiment was conducted to determine the effects of compound yeast cultures on the milk pro-duction,nitrogen excretion and blood biochemical parameters of dairy cows.A total of 24 Holstein lactating cows with similar parity,weight,milk yield and lactation stage (135±15 day in milk)were equally assigned to four groups,including one control group and 3 treatment groups,with dietary supplementation of compound yeast cultures at concentrations of 0,0.8%,1.0% and 1.2%,respectively.Milk yield,milk composition,ni-trogen excretion and blood biochemical parameters of each cow were recorded.The results showed:1 )The cows in the respective treatment groups had 8.48%,10.05%,8.97% higher daily milk yield than cows in the control

  1. Operative Links

    DEFF Research Database (Denmark)

    Wistoft, Karen; Højlund, Holger

    2012-01-01

    The article combines a public management perspective with a pedagogical perspective on health promotion. Our aim is to look into recent reforms in Denmark on health promotion in order to see how managerial ideas have been combined with welfare professional ideals concerning broad, positive and...... framework has three positions: first, a policy perspective on public organisations as networks, second, a semantic perspective on discourses and concepts of health and, third, a health pedagogical perspective on participation, intervention, and roles. Conclusion: it is necessary to establish ‘operative...... links' that indicate cooperative levels which facilitate a creative and innovative effort in disease prevention and health promotion targeted at children and adolescents - across traditional professional boundaries. It is proposed that such links are supported by network structures, shared semantics and...

  2. Operative Links

    DEFF Research Database (Denmark)

    Wistoft, Karen; Højlund, Holger

    2012-01-01

    educational approaches. Methods: Mixed qualitative design: survey based on telephone interviews with health managers (n=72), personal and focus group interviews with health professionals (n=84) and pupils (n=108) from 18 school classes, and comparative case studies in five selected municipalities of various...... educational goals, learning content, or value clarification. Health pedagogy is often a matter of retrospective rationalization rather than the starting point of planning. Health and risk behaviour approaches override health educational approaches. Conclusions: Operational links between health education...

  3. The effect of one session of supragingival plaque control on clinical and biochemical parameters of chronic periodontitis Efeito de uma única sessão de controle de placa supragengival nos parâmetros clínicos e bioquímicos da periodontite crônica

    Directory of Open Access Journals (Sweden)

    Érica Del Peloso Ribeiro

    2005-09-01

    Full Text Available Supragingival plaque control is a requisite for the success of any periodontal procedure. However, little is know about the effect of this procedure alone on periodontitis. The aim of this study was to determine the effect of supragingival plaque control on clinical and biochemical parameters of chronic periodontitis. Twenty-five subjects exhibiting at least 4 pockets > 5mm, non-smokers and with no relevant systemic diseases, were selected for the study. The clinical and biochemical assessments were done before and 21 days after removal of plaque retentive factors, extraction of affected teeth and instruction in oral hygiene. The statistical analysis was done with the Student paired t-test (pO objetivo deste estudo foi avaliar o efeito do controle de placa supragengival sobre os parâmetros clínicos e bioquímicos da periodontite crônica. Foram selecionados 25 pacientes apresentando pelo menos 4 sítios com profundidade de sondagem > 5 mm, não fumantes e sem alterações sistêmicas relevantes. A avaliação clínica e bioquímica foi feita antes e 21 dias após a remoção dos fatores retentivos de placa, exodontia dos dentes condenados e instrução de higiene bucal. A análise estatística foi feita através do teste t de Student pareado (p<0,05 e demonstrou redução da porcentagem de sítios com sangramento à sondagem de 42,72 ± 15,83 para 35,87 ± 13,30 (p=0,002. Redução na profundidade de sondagem foi observada nos dentes anteriores (média inicial/final: 2,88 mm/2,78 mm; p=0,01 e faces interproximais (média inicial/final: 3,80 mm/3,65 mm; p=0,001. Houve aumento de 66,81% para 68,65% (p=0,03 no número de sítios com profundidade de sondagem de 1 a 3 mm e redução de 26,21% para 24,17% (p=0,04 nos de 4 a 6 mm. O valor do BAPNA apresentou redução de 51,44 ± 20,78 para 38,64 ± 12,34 (p=0,04. Baseado nesses achados pôde-se concluir que o controle de placa supragengival tem efeitos benéficos, principalmente relacionados à redu

  4. Valores de referência de parâmetros bioquímicos no sangue de duas linhagens de camundongos Reference values for blood-based biochemical parameters in BALB/C and C57BL/6 wild-type mice

    Directory of Open Access Journals (Sweden)

    Aline Silva Almeida

    2008-12-01

    Full Text Available INTRODUÇÃO: Conhecer os parâmetros bioquímicos individuais de animais de laboratório utilizados na experimentação é importante, pois eles servirão como parâmetros para avaliar alterações funcionais em órgãos e como base para estabelecer valores de referência. OBJETIVO:Estabelecer valores de referência bioquímicos do sangue em camundongos das linhagens BALB/c e C57BL/6 selvagens do Biotério da Disciplina de Biologia Celular da Universidade Federal do Triângulo Mineiro (UFTM. Materiais e métodos: Foram utilizados 30 camundongos (BALB/c e C57BL/6 selvagem. Os exames realizados foram glicose, triglicérides, colesterol, proteínas totais, albumina, amilase, ácido úrico, uréia, fosfatase alcalina (kits Wiener, e as determinações foram realizadas no equipamento BIOPLUS-2000. RESULTADOS:Entre os nove analitos observou-se que quatro (albumina, glicose, proteínas totais e colesterol apresentaram diferenças estatisticamente significativas entre as linhagens. Padronizamos como valores de referência para os camundongos os valores do intervalo de confiança (IC. Nos analitos em que houve diferença significativa entre as linhagens (p INTRODUCTION: Identifying individual biochemical parameters of laboratory animal species is important inasmuch as they may be used in the evaluation of functional changes in organs and in the establishment of reference values. OBJECTIVE: To establish biochemical reference values for blood tests in BALB/c and C57BL/6 wild-type mice from the Vivarium of the Department of Cellular Biology at the Federal University of "Triângulo Mineiro". MATERIALS AND METHODS: Thirty wild-type mice of the lineages BALB/c and C57BL/6 were used to evaluate the serum levels of glucose, triglycerides, cholesterol, total protein, albumin, amylase, uric acid, urea and alkaline phosphatase. The determinations were performed in a BIOPLUS-2000 analyzer. Results: Four out of the nine analytes (albumin, glucose, total proteins and

  5. Parâmetros de bioquímica sérica de machos, fêmeas e filhotes de Araras canindé (Ara ararauna saudáveis mantidas em cativeiro comercial Serum biochemical parameters of healthy male, female and young blue-and-yellow macaws (Ara ararauna bred in captivity

    Directory of Open Access Journals (Sweden)

    Stella de Faria Valle

    2008-06-01

    , totaling nearly 80 species. The blue-and-yellow macaw (Ara ararauna, the most representative species of the Psittaciformes order, lives in the forest areas of different Brazilian regions. IBAMA, Brazilian environmental protection agency, has authorized the trade of wild animals raised in breeding facilities; consequently, their use as household pets has increased ever since. Currently, too little is known about clinical and laboratory parameters of wild species bred in captivity. The aim of the present study was to determine the serum biochemical parameters of healthy blue-and-yellow macaws (Ara ararauna of different sexes and ages reared in a commercial breeding facility under controlled and standardized feeding and management practices. Blood samples were collected from 35 blue-and-yellow macaws (11 fledglings and 24 adults and sent to the Laboratory of Veterinary Clinical Pathology of Universidade de Passo Fundo (UPF for measurement of the following biochemical parameters: uric acid (UA, albumin (Alb, aspartate aminotransferase (AST, calcium (Ca, cholesterol (Chol, creatine kinase (CK, alkaline phosphatase (AP, inorganic phosphorus (iP, fructosamine (Fru, gamma-glutamyl transferase (GGT, total protein (TP and urea nitrogen (Ur. There were significant differences among adult macaws with regard to CK levels (higher in males and to Ca and UA levels (higher in females. Differences were noted among adults and fledglings with respect to UA, Ca, Alb, Chol, AP, iP and Fru. Most of the findings can be ascribed to the different feeding regimens and ages of the birds analyzed. The data obtained herein can be used as reference parameters for Brazilian blue-and-yellow macaws.

  6. Hyponatraemia: biochemical and clinical perspectives.

    OpenAIRE

    Gill, G; Leese, G

    1998-01-01

    Hyponatraemia is a common bio-chemical abnormality, occurring in about 15% of hospital inpatients. It is often associated with severe illness and relatively poor outcome. Pathophysiologically, hyponatraemia may be spurious, dilutional, depletional or redistributional. Particularly difficult causes and concepts of hyponatraemia are the syndrome of inappropriate antidiuresis and the sick cell syndrome, which are discussed here in detail. Therapy should always be targeted at the underlying disea...

  7. Biochemical markers of bone turnover

    International Nuclear Information System (INIS)

    Biochemical markers of bone turnover has received increasing attention over the past few years, because of the need for sensitivity and specific tool in the clinical investigation of osteoporosis. Bone markers should be unique to bone, reflect changes of bone less, and should be correlated with radiocalcium kinetics, histomorphometry, or changes in bone mass. The markers also should be useful in monitoring treatment efficacy. Although no bone marker has been established to meet all these criteria, currently osteocalcin and pyridinium crosslinks are the most efficient markers to assess the level of bone turnover in the menopausal and senile osteoporosis. Recently, N-terminal telopeptide (NTX), C-terminal telopeptide (CTX) and bone specific alkaline phosphatase are considered as new valid markers of bone turnover. Recent data suggest that CTX and free deoxypyridinoline could predict the subsequent risk of hip fracture of elderly women. Treatment of postmenopausal women with estrogen, calcitonin and bisphosphonates demonstrated rapid decrease of the levels of bone markers that correlated with the long-term increase of bone mass. Factors such as circadian rhythms, diet, age, sex, bone mass and renal function affect the results of biochemical markers and should be appropriately adjusted whenever possible. Each biochemical markers of bone turnover may have its own specific advantages and limitations. Recent advances in research will provide more sensitive and specific assays

  8. Using pLink to Analyze Cross-Linked Peptides.

    Science.gov (United States)

    Fan, Sheng-Bo; Meng, Jia-Ming; Lu, Shan; Zhang, Kun; Yang, Hao; Chi, Hao; Sun, Rui-Xiang; Dong, Meng-Qiu; He, Si-Min

    2015-01-01

    pLink is a search engine for high-throughput identification of cross-linked peptides from their tandem mass spectra, which is the data-analysis step in chemical cross-linking of proteins coupled with mass spectrometry analysis. pLink has accumulated more than 200 registered users from all over the world since its first release in 2012. After 2 years of continual development, a new version of pLink has been released, which is at least 40 times faster, more versatile, and more user-friendly. Also, the function of the new pLink has been expanded to identifying endogenous protein cross-linking sites such as disulfide bonds and SUMO (Small Ubiquitin-like MOdifier) modification sites. Integrated into the new version are two accessory tools: pLabel, to annotate spectra of cross-linked peptides for visual inspection and publication, and pConfig, to assist users in setting up search parameters. Here, we provide detailed guidance on running a database search for identification of protein cross-links using the 2014 version of pLink. PMID:25754995

  9. Hematological, biochemical and ruminant parameters for diagnosis of left displacement of the abomasum in dairy cows from Southern Brazil Indicadores hematológicos, bioquímicos e ruminais no diagnóstico do deslocamento de abomaso à esquerda em vacas leiteiras do Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Felipe Cardoso de Cardoso

    2008-01-01

    Full Text Available The objective of this work was to evaluate hematological, biochemical and ruminant parameters for diagnosis and treatment of the left displaced abomasum (LDA in dairy cows, in the Plateau Region of Rio Grande do Sul, Brazil. Ruminant fluid, blood and urine samples were collected from 20 cows suffering LDA and from 20 healthy cows (control. The cows with LDA showed lower values of daily milk production, body weight and corporal condition score. The use of pH reagent strips showed to be functional in the field, when compared to a digital pH meter. Ruminant dynamics was damaged in cows affected by LDA, as it was evidenced by the higher reduction time of methylene blue. Serum values of lactate, beta-hydroxybutyrate, urea, albumin, free fatty acids and cholesterol shows to be auxiliary tools in the LDA characterization.O objetivo deste trabalho foi avaliar indicadores hematológicos, bioquímicos e ruminais no diagnóstico e tratamento do deslocamento de abomaso à esquerda (DAE em vacas leiteiras, na Região do Planalto do Rio Grande do Sul, Brasil. Foram coletadas amostras de líquido ruminal, sangue e urina de 40 animais, dos quais 20 vacas com DAE e 20 vacas clinicamente sadias utilizadas como grupo controle. Os animais com DAE, quando comparados ao grupo controle, apresentaram diminuição da produção de leite diária, do peso corporal e do escore condição corporal. A utilização de fitas reagentes para medição do pH ruminal demonstrou-se eficaz em campo, em comparação ao potenciômetro digital. A dinâmica ruminal apresentou-se prejudicada nos animais com DAE, o que foi evidenciado pelos valores aumentados do tempo de redução de azul de metileno. Os valores séricos de lactato, beta-hidroxibutirato, uréia, albumina, ácidos graxos livres e colesterol apresentam-se como ferramentas auxiliares na caracterização da doença.

  10. Effects of Dietary Protein Levels on Growth Performance, Digestibility of Nutrients, and Serum Biochemical Parameters in Growing Female Minks%饲粮蛋白质水平对育成期母貂生长性能、营养物质消化代谢及血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    张铁涛; 崔虎; 杨颖; 吴学壮; 高秀华; 杨福合; 邢秀梅

    2012-01-01

    本文旨在探讨饲粮蛋白质水平对育成期母貂生长性能、营养物质消化代谢及血清中与氮代谢相关的生化指标的影响.选择健康( 50±3)日龄断奶的雌性水貂60只,平均体重为(0.577±0.073)kg,随机分成6组,每组10个重复,每个重复1只,单笼饲养.6组水貂分别饲喂蛋白质水平为28%、30%、32%、34%、36%、38%的试验饲粮.在水貂65、80、95、110日龄时各进行1次消化代谢试验,分析各日龄水貂的蛋白质消化率、脂肪消化率、氮沉积、蛋白质生物学价值、饲料转化率及体增重等指标.结果表明:50~65日龄,38%蛋白质组体增重极显著高于28%和32%蛋白质组(P<0.01),38%蛋白质组饲料转化率极显著优于28%和32%蛋白质组(P<0.01);50~110日龄,32%和34%蛋白质组体增重显著高于28%蛋白质组(P<0.05).95日龄时,32%、34%和38%蛋白质组的蛋白质消化率极显著高于28%和30%蛋白质组(P<0.01).110日龄时,34%蛋白质组蛋白质生物学价值和净蛋白质利用率极显著低于其他试验组(P<0.01).110日龄时,38%蛋白质组尿素氮含量极显著高于其他各组(P<0.01);34%和36%蛋白质组总氨基酸含量显著高于28%蛋白质组(P<0.05).综合试验中的测定指标,建议50~65日龄,水貂饲粮蛋白质水平为36%~38%;66~80日龄,水貂饲粮蛋白质水平为34%~36%;81~110日龄,水貂饲粮蛋白质水平为32%~34%.%The purpose of this experiment was to study the regularity of growth performance, nutrient digestibility , and serum biochemical parameters with different dietary protein levels in growing female minks (Muste-la vison). Sixty aged (50 ?) days female minks were randomly divided into six groups according to a single-factor design. Each group included ten replications in which one mink fed in a single cage. The minks were fed experimental diets with protein levels of 28% , 30% , 32% , 34% , 36

  11. Biological and biochemical properties in evaluation of forest soil quality

    OpenAIRE

    Błońska Ewa; Lasota Jarosław

    2014-01-01

    The aim of this study was to assess the possibility of using biological and biochemical parameters in the evaluation of forest soil quality and changes caused by land use. The study attempted to determine a relationship between the enzymatic activity of soil, the number of earthworms and soil physico-chemical properties. The study was carried out in central Poland in adjoining Forest Districts (Przedbórz and Smardzewice). In soil samples taken from 12 research plots, basic physico-chem...

  12. Automatising the analysis of stochastic biochemical time-series

    OpenAIRE

    Caravagna, Giulio; De Sano, Luca; Antoniotti, Marco

    2015-01-01

    Background Mathematical and computational modelling of biochemical systems has seen a lot of effort devoted to the definition and implementation of high-performance mechanistic simulation frameworks. Within these frameworks it is possible to analyse complex models under a variety of configurations, eventually selecting the best setting of, e.g., parameters for a target system. Motivation This operational pipeline relies on the ability to interpret the predictions of a model, often represented...

  13. Dancing links

    CERN Document Server

    Knuth, Donald E

    2009-01-01

    The author presents two tricks to accelerate depth-first search algorithms for a class of combinatorial puzzle problems, such as tiling a tray by a fixed set of polyominoes. The first trick is to implement each assumption of the search with reversible local operations on doubly linked lists. By this trick, every step of the search affects the data incrementally. The second trick is to add a ghost square that represents the identity of each polyomino. Thus puts the rule that each polyomino be used once on the same footing as the rule that each square be covered once. The coding simplifies to a more abstract form which is equivalent to 0-1 integer programming. More significantly for the total computation time, the search can naturally switch between placing a fixed polyomino or covering a fixed square at different stages, according to a combined heuristic. Finally the author reports excellent performance for his algorithm for some familiar puzzles. These include tiling a hexagon by 19 hexiamonds and the N queen...

  14. Comparison of Nutritional Parameters among Adult and Elderly Hemodialysis Patients

    OpenAIRE

    Gülperi Çelik, Bahar Oc, Inci Kara, Mümtaz Yılmaz, Ali Yuceaktas, Seza Apiliogullari

    2011-01-01

    Aim: The aim of this study was to compare the nutritional biochemical parameters, prealbumin levels, and bioimpedance analysis parameters of adult and elderly hemodialysis (HD) patients.Methods: This prospective cross-sectional study included 50 adult HD patients (42.0 % female). Nutritional status was assessed by post-dialysis multifrequency bioimpedance analysis (BIA), serum prealbumin and other nutritional biochemical parameters.Results: Mean age of patients was 57.4±15.1 years (range...

  15. Comparison of Nutritional Parameters among Adult and Elderly Hemodialysis Patients

    OpenAIRE

    Çelik, Gülperi; Oc, Bahar; Kara, Inci; Yılmaz, Mümtaz; Yuceaktas, Ali; Apiliogullari, Seza

    2011-01-01

    Aim: The aim of this study was to compare the nutritional biochemical parameters, prealbumin levels, and bioimpedance analysis parameters of adult and elderly hemodialysis (HD) patients. Methods: This prospective cross-sectional study included 50 adult HD patients (42.0 % female). Nutritional status was assessed by post-dialysis multifrequency bioimpedance analysis (BIA), serum prealbumin and other nutritional biochemical parameters. Results: Mean age of patients was 57.4±15.1 years (range: 3...

  16. Reference values for chinchilla (Chinchilla laniger blood cells and serum biochemical parameters Valores de referência para os parâmetros das células e bioquímica sangüínea da chinchila (Chinchilla laniger

    Directory of Open Access Journals (Sweden)

    Tális de Oliveira Silva

    2005-06-01

    Full Text Available Raising chinchilla (Chinchilla laniger for commercial purpose has increased significantly; however, hematological and serum biochemical reference values have not yet been determined for chinchillas raised in south Brazil. Establishing blood cells and serum biochemistry reference values might be helpful to evaluate health status of chinchillas and might be used as a tool by clinicians. The purpose of this study was to determine the reference values for blood cells and serum biochemistry of Chinchilla laniger. Blood samples were collected by cardiac puncture from 16 adult males, at the time they were killed to remove the fur coat, and from 8 adult males anesthetized with ketamine and xylazine. Blood cell counts and serum biochemistry analysis were performed using standard techniques and the results were expressed as mean ± SEM. Analysis of blood parameters from post-mortem cardiac punctured and from anesthetized chinchillas indicated that blood samples from anesthetized chinchillas had higher PCV, Hemoglobin, MCHC and WBC (P A criação de chinchila (Chinchilla laniger com objetivos comerciais tem crescido muito nos últimos anos. No entanto, os valores de referência para os parâmetros hematológicos e bioquímicos não foram ainda determinados para chinchilas criados no sul do Brasil. O estabelecimento dos valores de referência para esses parâmetros pode servir de auxílio para a avaliação da saúde das chinchilas e servir de auxílio diagnóstico para o clínico. Esse estudo teve como objetivo determinar os valores de referência das células e da bioquímica sangüínea da Chinchilla laniger. As amostras de sangue foram coletadas por meio de punção cardíaca de 16 machos adultos no momento em que os animais foram mortos para remoção da pele, e de 8 machos adultos após anestesia com xylazina e ketamina. A contagem das células sangüíneas e a análise dos parâmetros bioquímicos foram feitas utilizando-se métodos padronizados em

  17. CPM模型优化饲粮对7~15月龄后备荷斯坦母牛生长发育和血液生化指标影响%Effects of Diets Optimized by CPM Model on Growth, Development and Blood Biochemical Parameters of Holstein Replacement Heifers at 7 to 15 Months of Age

    Institute of Scientific and Technical Information of China (English)

    王洋; 曲永利; 王志博; 刘立成; 刘翔; 赵鑫; 邢伟明

    2014-01-01

    本文利用CPM模型优化规模化奶牛场7~15月龄后备母牛饲粮,研究优化后饲粮对后备母牛生长发育和血液生化指标的影响。选用相同月龄(7月龄),胎次、体高和体重相近的健康后备荷斯坦母牛40头,按照配对试验设计把试验奶牛随机分为2组,每组20头,对照组采用奶牛场现有的饲粮配方,试验组采用 CPM模型优化后的饲粮配方。试验期为270 d。结果表明:饲喂CPM模型优化饲粮极显著提高了后备母牛8、10、11、13和15月龄的管围( P<0.01);试验组后备母牛血清中钙含量在8月龄时显著高于对照组( P<0.05);优化后7月龄、8~9月龄、10~12月龄、13~15月龄饲粮成本比调整前分别降低了1.02、0.78、0.62和0.52元/(头·d)。由此可见,饲喂CPM模型优化饲粮可提高后备母牛的管围,促进后备母牛的生长,促进血钙的吸收,降低饲养成本,CPM模型可用于评价和优化后备荷斯坦母牛的饲粮配方。%Diets of Holstein replacement heifers in scaled farm was optimized by CPM model, and the effects of optimized diets on growth, development and blood biochemical parameters were evaluated in this study. For-ty Holstein replacement heifers with the same months of age ( 7 months of age) , and similar body height and weight were randomly divided into control and test groups according to paired experimental design, and each group had 20 heifers. Heifers of control group were fed original diets of the farm, while those of test group were fed diets optimized by CPM model. The experimental period was 270 days. The results showed as fol-lows: replacement heifers fed optimized diets had significantly higher cannon circumference at 8, 10, 11, 13 and 15 months of age ( P<0.01);serum calcium content of replacement heifers fed optimized diets was signifi-cantly higher than that of heifers fed original diets ( P<0.05);cost of optimized diets of 7

  18. Efeitos do aumento na sobrecarga de treinamento sobre parâmetros bioquímicos e hormonais em ratos Efectos del aumento de sobrecarga de entrenamiento sobre parámetros bioquímicos y hormonales en ratones Effects of increase of overload training on biochemical and hormonal parameters in rats

    Directory of Open Access Journals (Sweden)

    Ronaldo Vagner Thomatieli Santos

    2006-06-01

    Full Text Available Para o treinamento ser bem sucedido deve ser suficientemente intenso para provocar a quebra da homeostase, a adaptação e, por fim, a supercompensação. Todavia, condições de estresse excessivo induzido pelo exercício físico podem provocar efeitos indesejáveis. Este trabalho tem como objetivo avaliar se o aumento na sobrecarga de treinamento altera parâmetros hormonais e bioquímicos similares ao overreaching. Os animais foram divididos em três grupos: SED (animais sedentários, MOD (animais que treinaram de forma moderada durante seis semanas e grupo EXT (que treinaram de forma semelhante ao grupo MOD por quatro semanas, duas sessões diárias de treinamento na quinta semana e três sessões na sexta semana. Houve aumento da concentração plasmática de glutamato no grupo EXT (p Para que el entrenamiento tenga éxito, éste debe ser lo suficientemente intenso para provocar la quiebra de homeostasis, la adaptación y por fin la supercompensación. Sin embargo, condiciones de estrés excesivo inducidos por el ejercicio físico pueden provocar efectos indeseables. Este trabajo tiene como objetivo evaluar si el aumento en la sobrecarga de entrenamiento altera parámetros hormonales y bioquímicos similares al overreaching. Los animales fueron divididos en tres grupos: SED (animales sedentarios, MOD (animales que entrenaron de forma moderada durante 6 semanas y EXT (que entrenaron de forma semejante al grupo MOD por 4 semanas, 2 sesiones diarias de entrenamiento en la quinta semana y 3 sesiones la sexta semana. Hubo aumento en la concentración plasmática de glutamato en el grupo EXT (p The training will be efficient if it is intensive enough to promote homeostasis break, adaptation and super compensation consequently. On the other hand, excessive stress conditions induced by exercise may promote undesirable effects. This paper aims to evaluate the effects of the increase in overload training upon some hormonal and biochemical parameters

  19. Determinación de precisión de la eritrosedimentación y su relación con otros parámetros hematológicos y bioquímicos en bóvidos - Determining the precision of the erythrosedimentation and its relation with other hematologic and biochemical parameters in bovines

    Directory of Open Access Journals (Sweden)

    Paiva, Rafael

    2010-03-01

    precision of theerythrosedimentation and its behavior in front of other hematologicand/or biochemical parameters in samples of buffaloes and Caroracattle localized in the state of Mérida-Venezuela. Theerythrosedimentation was manually performed by using the methodsand procedures suggested for human beings (Wintrobe andWestergreen.The hemoglobin, the hematocrit and the number of leucocytes weredetermined by the use of the IDEXX Vetautoread™ HematologyAnalyzer. The fibrinogen concentration was determined by the use of aBio-Bas coagulometer. The concentration of triglycerides andcholesterol was obtained in a Spectronic Genesys 20. The dataobtained were processed by the statistical program Statistical Packagefor the Social Sciences version 10. Resulting the Wintrobe method inthe buffaloes, an F test (p<0, 05 as the coefficient of variation <5%; 2and the Westergreen method for the Carora cattle in the second hourof erythrosedimentation, an F test (p<0, 05 as the coefficient ofvariation <5%. It can be concluded that, the erythrosedimentationpresented precision by/in the Wintrobe method in buffaloes; meanwhilethe Westergreen method presented the precision in the second hour inthe Carora cattle. So that, we recommend that every locality determine the values of the erythrosedimentation depending/based onthe method and the bovine because the particular hematologic andbiochemical variations of each specie have influence on the precision of the method.

  20. Hematological, Serum Biochemical and Trace Mineral Indices of Cattle with Foreign Body Rumen Impaction

    OpenAIRE

    J.F. Akinrinmade; A.S. Akinrinde

    2012-01-01

    This study was aimed at investigating the hematological, biochemical and trace mineral status of cattle with foreign body rumen impaction. Hematological, serum biochemical and trace mineral parameters of cattle with Foreign Body Rumen impaction (FBR) and normal cattle Without Foreign Body Rumen impaction (WFBR) were analyzed in order to evaluate their influence as risk factors in the etio-pathogenesis of non-penetrating foreign body rumen impaction. Blood samples with appropriate preservative...

  1. Mercury Induced Biochemical Alterations As Oxidative Stress In Mugil Cephalus In Short Term Toxicity Test

    OpenAIRE

    J.S.I Rajkumar; Samuel Tennyson

    2013-01-01

    Mugil cephalus juveniles of size 2.5 ±0.6cm were exposed to mercury in short term chronic toxicity test through static renewal bioassay to detect the possible biochemical agent as biomarkers in aquatic pollution and in estuarine contamination as specific. Lipid peroxidation levels, glutathione S -transferase, catalase, reduced glutathione and acetylcholinesterase were studied as biochemical parameters. Increased thio-barbituric acid reactive substances levels were observed under exposur...

  2. Role of Rosemary leaves extract against radiation-induced hematological and biochemical alterations in mice

    OpenAIRE

    Acharya Garima S.; Goyal Pradeep K.

    2008-01-01

    The present paper is a study of the modulatory effect of Rosmarinus officinalis leaves extract on radiation-induced hematological and biochemical changes in Swiss albino mice. The dose reduction factor for the Rosemary extract against gamma rays was calculated 1.53 from LD50/30 values. The Rosemary extract was administered orally for 5 consecutive days prior to radiation exposure. The hematological and biochemical parameters were assessed from day 1 to 30 post-irradiation intervals. The total...

  3. Quantitative Morphological and Biochemical Studies on Human Downy Hairs using 3-D Quantitative Phase Imaging

    OpenAIRE

    Lee, Sangyun; Kim, Kyoohyun; Lee, Yuhyun; Park, Sungjin; Shin, Heejae; Yang, Jongwon; Ko, Kwanhong; Park, HyunJoo; Park, YongKeun

    2015-01-01

    This study presents the morphological and biochemical findings on human downy arm hairs using 3-D quantitative phase imaging techniques. 3-D refractive index tomograms and high-resolution 2-D synthetic aperture images of individual downy arm hairs were measured using a Mach-Zehnder laser interferometric microscopy equipped with a two-axis galvanometer mirror. From the measured quantitative images, the biochemical and morphological parameters of downy hairs were non-invasively quantified inclu...

  4. Sex-linked dominant

    Science.gov (United States)

    Inheritance - sex-linked dominant; Genetics - sex-linked dominant; X-linked dominant; Y-linked dominant ... type of chromosome that is affected (autosomal or sex chromosome). It also depends on whether the trait ...

  5. Sex-linked dominant

    Science.gov (United States)

    Inheritance - sex-linked dominant; Genetics - sex-linked dominant; X-linked dominant; Y-linked dominant ... can be either an autosomal chromosome or a sex chromosome. It also depends on whether the trait ...

  6. Structural, biochemical, cellular, and functional changes in skeletal muscle extracellular matrix with aging

    DEFF Research Database (Denmark)

    Kragstrup, Tue Wenzel; Kjaer, M; Mackey, A L

    2011-01-01

    The extracellular matrix (ECM) of skeletal muscle is critical for force transmission and for the passive elastic response of skeletal muscle. Structural, biochemical, cellular, and functional changes in skeletal muscle ECM contribute to the deterioration in muscle mechanical properties with aging....... Structural changes include an increase in the collagen concentration, a change in the elastic fiber system, and an increase in fat infiltration of skeletal muscle. Biochemical changes include a decreased turnover of collagen with potential accumulation of enzymatically mediated collagen cross-links and a...... buildup of advanced glycation end-product cross-links. Altered mechanotransduction, poorer activation of satellite cells, poorer chemotactic and delayed inflammatory responses, and a change in modulators of the ECM are important cellular changes. It is possible that the structural and biochemical changes...

  7. Biochemical Analysis of Microbial Rhodopsins.

    Science.gov (United States)

    Maresca, Julia A; Keffer, Jessica L; Miller, Kelsey J

    2016-01-01

    Ion-pumping rhodopsins transfer ions across the microbial cell membrane in a light-dependent fashion. As the rate of biochemical characterization of microbial rhodopsins begins to catch up to the rate of microbial rhodopsin identification in environmental and genomic sequence data sets, in vitro analysis of their light-absorbing properties and in vivo analysis of ion pumping will remain critical to characterizing these proteins. As we learn more about the variety of physiological roles performed by microbial rhodopsins in different cell types and environments, observing the localization patterns of the rhodopsins and/or quantifying the number of rhodopsin-bearing cells in natural environments will become more important. Here, we provide protocols for purification of rhodopsin-containing membranes, detection of ion pumping, and observation of functional rhodopsins in laboratory and environmental samples using total internal reflection fluorescence microscopy. © 2016 by John Wiley & Sons, Inc. PMID:27153387

  8. Parameter Estimation

    DEFF Research Database (Denmark)

    Sales-Cruz, Mauricio; Heitzig, Martina; Cameron, Ian;

    2011-01-01

    In this chapter the importance of parameter estimation in model development is illustrated through various applications related to reaction systems. In particular, rate constants in a reaction system are obtained through parameter estimation methods. These approaches often require the application...... of optimisation techniques coupled with dynamic solution of the underlying model. Linear and nonlinear approaches to parameter estimation are investigated. There is also the application of maximum likelihood principles in the estimation of parameters, as well as the use of orthogonal collocation to...... generate a set of algebraic equations as the basis for parameter estimation.These approaches are illustrated using estimations of kinetic constants from reaction system models....

  9. INVESTIGATIONS ON BIOCHEMICAL PURIFICATION OF GROUND WATER FROM HYDROGEN SULFIDE

    Directory of Open Access Journals (Sweden)

    Yu. P. Sedlukho

    2015-01-01

    Full Text Available The paper considers problems and features of biochemical removal of hydrogen sulfide from ground water. The analysis of existing methods for purification of ground water from hydrogen sulfide has been given in the paper. The paper has established shortcomings of physical and chemical purification of ground water. While using aeration methods for removal of hydrogen sulfide formation of colloidal sulfur that gives muddiness and opalescence to water occurs due to partial chemical air oxidation. In addition to this violation of sulfide-carbonate equilibrium taking place in the process of aeration due to desorption of H2S and CO2, often leads to clogging of degasifier nozzles with formed CaCO3 that causes serious operational problems. Chemical methods require relatively large flow of complex reagent facilities, storage facilities and transportation costs.In terms of hydrogen sulfide ground water purification the greatest interest is given to the biochemical method. Factors deterring widespread application of the biochemical method is its insufficient previous investigation and necessity to execute special research in order to determine optimal process parameters while purifying groundwater of a particular water supply source. Biochemical methods for oxidation of sulfur compounds are based on natural biological processes that ensure natural sulfur cycle. S. Vinogradsky has established a two-stage mechanism for oxidation of hydrogen sulfide with sulfur bacteria (Beggiatoa. The first stage presupposes oxidation of hydrogen sulphide to elemental sulfur which is accumulating in the cytoplasm in the form of globules. During the second stage sulfur bacteria begin to oxidize intracellular sulfur to sulfuric acid due to shortage of hydrogen sulfide.The paper provides the results of technological tests of large-scale pilot plants for biochemical purification of groundwater from hydrogen sulfide in semi-industrial conditions. Dependences of water quality

  10. Análisis de parámetros bioquímicos en grandes quemados: nuevos factores pronósticos. De la investigación básica a la clínica Analysis of biochemical parameters in major burn patients: new prognostic factors. From the basic to the clinical research

    Directory of Open Access Journals (Sweden)

    A.A. Maldonado

    2012-12-01

    the influence of ions and proteins applied to 143 patients in the Major Burn Patient Unit in the Unfallkrankenhaus Hospital in Berlin (Germany. A statistical study is carried out to define patient mortality once the previous calculations have been performed, considering: 1 their biochemical parameters in the first 48 hours, 2 their clinical factors: age, total burned surface area, depth of the burn, presence of inhalation syndrome, etc. A logistic regression analysis was made using these data calculating the mortality predictive function, which was validated by a group of 35 new patients. The importance of each plasmatic ion and proteins was calculated in the electrical balance of the patients with major burns. Age, burned surface area, pH and [Mg2+] were introduced for the study of mortality in these patients via statistical analysis. From the study it was confirmed that anions [HCO3¯] and [Cl¯], together with cation [Na+] are the ions with most influence on the plasmatic balance of major burn patients. Protein concentration has a lower incidence on this balance: a loss of 50 % in albumin concentration during the first 48 hours is equivalent to a loss of 1 mmol/l in the [HCO3¯] concentration. It can also be stated that the use of parameters pH and [Mg2+] can improve mortality prediction in these patients.

  11. Universally Sloppy Parameter Sensitivities in Systems Biology

    OpenAIRE

    Gutenkunst, Ryan N.; Waterfall, Joshua J.; Casey, Fergal P.; Brown, Kevin S.; Christopher R. Myers; Sethna, James P.

    2007-01-01

    Quantitative computational models play an increasingly important role in modern biology. Such models typically involve many free parameters, and assigning their values is often a substantial obstacle to model development. Directly measuring in vivo biochemical parameters is difficult, and collectively fitting them to other experimental data often yields large parameter uncertainties. Nevertheless, in earlier work we showed in a growth-factor-signaling model that collective fitting could yield...

  12. Serum Biochemical Phenotypes in the Domestic Dog

    Science.gov (United States)

    Chang, Yu-Mei; Hadox, Erin; Szladovits, Balazs; Garden, Oliver A.

    2016-01-01

    The serum or plasma biochemical profile is essential in the diagnosis and monitoring of systemic disease in veterinary medicine, but current reference intervals typically take no account of breed-specific differences. Breed-specific hematological phenotypes have been documented in the domestic dog, but little has been published on serum biochemical phenotypes in this species. Serum biochemical profiles of dogs in which all measurements fell within the existing reference intervals were retrieved from a large veterinary database. Serum biochemical profiles from 3045 dogs were retrieved, of which 1495 had an accompanying normal glucose concentration. Sixty pure breeds plus a mixed breed control group were represented by at least 10 individuals. All analytes, except for sodium, chloride and glucose, showed variation with age. Total protein, globulin, potassium, chloride, creatinine, cholesterol, total bilirubin, ALT, CK, amylase, and lipase varied between sexes. Neutering status significantly impacted all analytes except albumin, sodium, calcium, urea, and glucose. Principal component analysis of serum biochemical data revealed 36 pure breeds with distinctive phenotypes. Furthermore, comparative analysis identified 23 breeds with significant differences from the mixed breed group in all biochemical analytes except urea and glucose. Eighteen breeds were identified by both principal component and comparative analysis. Tentative reference intervals were generated for breeds with a distinctive phenotype identified by comparative analysis and represented by at least 120 individuals. This is the first large-scale analysis of breed-specific serum biochemical phenotypes in the domestic dog and highlights potential genetic components of biochemical traits in this species. PMID:26919479

  13. Biochemical bases of mineral waters genesis

    Directory of Open Access Journals (Sweden)

    D. D. Zhernosekov

    2005-02-01

    Full Text Available This work directs data about mineral water genesis. The accent on balneological sense is done. We suggest the criteria of biochemical processes estimation which take part in mineral water compounds creation. These criteria can be used for illustration of dependence between waters medical properties and biochemical processes of their genesis.

  14. The Cytochrome P450 Engineering Database: integration of biochemical properties

    Directory of Open Access Journals (Sweden)

    Lisitsa Andrey

    2009-11-01

    Full Text Available Abstract Background Cytochrome P450 monooxygenases (CYPs form a vast and diverse enzyme class of particular interest in drug development and a high biotechnological potential. Although very diverse in sequence, they share a common structural fold. For the comprehensive and systematic comparison of protein sequences and structures the Cytochrome P450 Engineering Database (CYPED was established. It was built up based on an extensible data model that enables its functions readily enhanced. Description The new version of the CYPED contains information on sequences and structures of 8613 and 47 proteins, respectively, which strictly follow Nelson's classification rules for homologous families and superfamilies. To gain biochemical information on substrates and inhibitors, the CYPED was linked to the Cytochrome P450 Knowledgebase (CPK. To overcome differences in the data model and inconsistencies in the content of CYPED and CPK, a metric was established based on sequence similarity to link protein sequences as primary keys. In addition, the annotation of structurally and functionally relevant residues was extended by a reliable prediction of conserved secondary structure elements and by information on the effect of single nucleotide polymorphisms. Conclusion The online accessible version of the CYPED at http://www.cyped.uni-stuttgart.de provides a valuable tool for the analysis of sequences, structures and their relationships to biochemical properties.

  15. Murine interleukin 2 receptor. IV. Biochemical characterization

    International Nuclear Information System (INIS)

    The IL 2 receptor isolated from the IL 2-dependent CTL-L cell line was subjected to biochemical analysis. Pulse-chase and tunicamycin studies, as well as digestion with the endoglycosidases, Endo-F and Endo-H, of 35S-methionine-labeled IL 2 receptors suggested a single protein pecursor of 32,000 (p32) daltons. The p32 precursor was rapidly processed by addition of high-mannose-containing core N-linked sugars to intracytoplasmic precursor intermediates of 38,000 (p38) and 40,000 (p40) daltons, which undergo further processing to yield a mature surface receptor with heterogeneous apparent m.w. of 52,000 to 65,000 (p58). Two-dimensional gel studies indicated that p58 exhibited broad charge heterogeneity between pH 4.6 and 6.3. Endo-F digestions of p58 shifted the isoelectric focus point to a more basic 5.5 to 7.4. This considerable charge heterogeneity is consistent with the possibility that other post-translational modifications to the mouse IL 2 receptor occur besides addition of complex N-linked glycans. Immunoprecipitations of the IL 2 receptor from surface iodinated cells also revealed an additional band at 110,000 (p110) daltons. IEF vs SDS-PAGE two-dimensional gel studies demonstrated that p110 also had an isoelectric focus point identical to p58. Western blot studies with an anti-IL 2 receptor monoclonal antibody (7D4) demonstrates that p38, p40, p58, and p110 each expressed the epitope recognized by this antibody. Thus, it is likely that p110 is not a unique molecule that coprecipitates with IL 2 receptor. Western blot analysis of mitogen-stimulated T and B lymphocytes also revealed bands similar to p58 and p110, although these bands had an average apparent m.w. 3000 and 6000 less than those seen for CTL-L cells

  16. Seroprevalence of brucellosis and associated hemato-biochemical changes in pakistani horses

    International Nuclear Information System (INIS)

    The aim of this study was to determine the seroprevalence and hemato-biochemical manifestations of brucellosis in horses. Serum samples were screened for Brucella antibodies by Rose Bengal plate test (RBPT) and serum agglutination test (SAT). Blood samples were evaluated for hemato-biochemical parameters following standard procedures. Results indicated seroprevalence of brucellosis 20.13 and 16.23% in horses by RBPT and SAT, respectively. Brucellosis does not lead to any significant change in hematological and biochemical parameters in relation to age, sex, body condition and lactation except few parameters. The values of erythrocyte sedimentation rate, neutrophil, basophil and alkaline phosphatase significantly decreased in brucellosis positive animals as compared to healthy animals whereas lymphocytes and alanine aminotransferase were in opposite order. It was concluded from the results that prevalence of brucellosis in horse population is of concern; therefore, control measures should be opted so that its zoonotic threat is curtailed. (author)

  17. Characterization of a Functional Hydrogel Layer on a Silicon-Based Grating Waveguide for a Biochemical Sensor

    OpenAIRE

    Hong, Yoo-Seung; Kim, Jongseong; Sung, Hyuk-Kee

    2016-01-01

    We numerically demonstrated the characteristics of a functional hydrogel layer on a silicon-based grating waveguide for a simple, cost-effective refractive index (RI) biochemical sensor. The RI of the functional hydrogel layer changes when a specific biochemical interaction occurs between the hydrogel-linked receptors and injected ligand molecules. The transmission spectral profile of the grating waveguide shifts depends on the amount of RI change caused by the functional layer. Our character...

  18. CPM模型优化饲粮对16~24月龄后备荷斯坦母牛生长发育和血清生化指标的影响%Effects of Diets Optimized by CPM Model on Growth Development and Serum Biochemical Parameters of Holstein Replacement Heifers at 16 to 24 Months of Age

    Institute of Scientific and Technical Information of China (English)

    韩云胜; 曲永利; 袁雪; 王志博; 王洋; 刘立成; 李伟; 殷溪瀚

    2015-01-01

    本文旨在研究应用CPM模型优化规模化奶牛场16~24月龄后备母牛饲粮对该阶段母牛生长发育及血清生化指标的影响. 选择体重和体高相近的相同月龄( 16月龄)荷斯坦后备母牛40头,将试验奶牛随机分为对照组和试验组,每组各20头,对照组后备母牛饲喂牛场当前饲粮,试验组后备母牛饲喂CPM模型优化后的饲粮,试验期从16月龄开始止于24月龄,共270 d.结果表明:饲喂经CPM模型优化的饲粮显著提高了17月龄后备母牛的管围( P<0.05);试验组后备母牛的管围指数在17、18、20、21、23和24月龄时显著高于对照组(P<0.05),胸围指数在17和18月龄时显著高于对照组( P<0.05). 试验组后备母牛血清中钙的含量在17、23、24月龄时显著高于对照组( P<0.05) ,血清中磷的含量在16月龄时极显著地高于对照组( P<0.01). 经CPM模型优化后,16~22 月龄和23~24 月龄的饲粮成本分别降低了1. 03、0. 32 元/d. 饲喂CPM模型优化的饲粮在一定的范围内促进了后备母牛的生长发育,同时降低了饲养成本. 因此,CPM模型可用于16~24月龄荷斯坦后备母牛饲粮配方的评价和优化.%The objective of this study was conducted to determine the effects of diets optimized by CPM model on growth development and serum biochemical parameters of replacement heifers at 16 to 24 months of age. Forty 16-month-old Holstein replacement heifers with similar body weight and body height were selected and randomly divided into control and test groups, and each group included 20 Holstein replacement heifers. The heifers of control group were fed the present diet in farm, and those of test group were fed diets optimized by CPM model. The experimental period was 270 d during 16 to 24 months of age of Holstein replacement heifers. Results: the replacement heifers fed optimized diets significantly increased the cannon circumference at 17 month of age ( P<0.05) . The replacement heifers of test group

  19. Effects of different dietary carbohydrate levels on growth performance and blood biochemical parameters of juvenile GIFT tilapia(Oreochromis niloticus)%饲料碳水化合物水平对吉富罗非鱼幼鱼生长性能和血液主要生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    吴凡; 文华; 蒋明; 刘伟; 刘波; 田娟; 杨长庚

    2012-01-01

    【Objective】 This study investigated the influences of different dietary carbohydrate(CBH) levels on growth performance,body composition and blood biochemical parameters of juvenile GIFT tilapia(Oreochromis niloticus),to detect the suitable addition amount of CBH to the diet of GIFT tilapia.【Method】 5 iso-lipidic(7.0% crude lipid) diet groups were formulated with different CBH mass fractions:20%,27%,34%,41%,and 48%,respectively.Each diet was randomly fed to triplicate groups of 30 juvenile GIFT tilapia with average weights of(4.77±0.52) g for 10 weeks.【Result】 The weight gain rates(WGR) and specific growth rates(SGR) of the diet group with 41% CBH were significant higher than those of the other four groups(P0.05).The feed conversion rates(FCR) of the diet group with 27% CBH were the lowest in the all groups.The hepatopancreas somatic indexes(HSI) and viscerosomatic indexes(VSI) of test fishes increased significantly as the increase of dietary CBH levels.Protein efficiency ratios(PER) and protein productive values(PPV) of the diet group with 41% CBH were significant higher than those of groups with 20%,27% and 34% CBH(P0.05).Crude protein contents in whole-body and liver of fishes decreased as the increase of dietary CBH levels,while body and liver lipid contents showed converse trends.The contents of serum cholesterol(CHO) and triglyceride(TG) and the activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) of the fishes fed with 48% CBH diet were significant higher than other treatments.【Conclusion】 The results indicated that dietary CBH could reduce the protein dosage for juvenile GIFT tilapia and the suggested optimal dietary CBH level was 41%.However,exorbitant dietary CBH intake(such as 48%) might result in liver fat accumulation,functional disorder and hyperlipemia.%【目的】研究不同碳水化合物(CBH)水平对吉富罗非鱼幼鱼生长性能、体成分和血液生化指标的影响,以确定其适宜添

  20. 包膜复合酸化剂对东乡绿壳蛋鸡生产性能、矿物元素代谢及血清生化指标的影响%Effects of Coated Compound Acidifiers on Performance, Metabolism of Mineral Elements, and Serum Biochemical Parameters of Dongxiang Green Shell Laying Hens

    Institute of Scientific and Technical Information of China (English)

    魏艳红; 曲湘勇; 蔡超; 杨清丽; 徐勋; 肖建新; 申光荣

    2015-01-01

    本试验旨在研究包膜复合酸化剂对东乡绿壳蛋鸡生产性能、矿物元素代谢及血清生化指标的影响。选用48周龄健康东乡黑羽绿壳蛋鸡300羽,随机分为5组,每组5个重复,每个重复12羽,分别饲喂在基础饲粮中添加0(对照)、100、250、500和1000 mg/kg的包膜复合酸化剂的试验饲粮,试验期28 d。结果表明:与对照组相比,1)500和1000 mg/kg组可显著提高产蛋率(P<0.05),显著降低料蛋比(P<0.05);2)250 mg/kg组显著提高了蛋黄指数(P<0.05),500 mg/kg组显著提高蛋壳厚度(P<0.05);3)100 mg/kg组显著或极显著提高了蛋鸡血清总蛋白( P<0.01)、白蛋白( P<0.05)和葡萄糖含量( P<0.05),250 mg/kg组血清总蛋白( P<0.05)、尿酸含量(P<0.05)和白蛋白/球蛋白(P<0.01)显著或极显著上升,500 mg/kg组白蛋白/球蛋白极显著提高( P<0.01),1000 mg/kg组血清钙含量显著提高( P<0.05);4)酸化剂可显著提高蛋黄中铜含量( P<0.05),500和1000 mg/kg组蛋黄中锰含量极显著提高( P<0.01),1000 mg/kg组粪中锰含量极显著降低(P<0.01),250、500和1000 mg/kg组粪中镁含量极显著降低(P<0.01)。由此可见,产蛋期东乡绿壳蛋鸡饲粮中添加250~500 mg/kg包膜复合酸化剂为宜。%This study was conducted to explore the effects of coated compound acidifiers on performance, me⁃tabolism of mineral elements and serum biochemical parameters in Dongxiang green shell laying hens. A total of 300 healthy 48⁃week⁃old Dongxiang green shell laying hens were obtained and randomly divided into 5 groups with 5 replicates per group and 12 hens per replicate. The test lasted for 28 days. Diets of 5 groups in⁃cluded a basal diet supplemented with 0 ( control) , 100, 250, 500 and 1 000 mg/kg coated compound acidifi

  1. Mixed Silage of Corncob and Citrus Pulp Affects Growth Performance and Serum Biochemical Parameters of Beef Cattle%玉米芯-柑橘渣混合青贮料对肉牛生长性能和血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    志莉; 薛白; 王之盛; 蔡义民; 刘振龙; 邓华

    2011-01-01

    本文旨在研究玉米芯-柑橘渣混合青贮料对肉牛生长性能、养分消化率和血清生化指标的影响.选择24头平均体重(173.13±5.10)kg的健康西门塔尔杂种肉牛,随机分为4个处理,每个处理6个重复,每个重复l头牛,饲养时间为50 d.4个处理分别是:1)对照组(CON),40%精料+60%牛鞭草;2)试验l组(S20),40%精料+40%牛鞭草+20%青贮料(玉米芯-柑橘渣混合青贮料);3)试验2组(S30),40%精料+30%牛鞭草+30%青贮料;4)试验3组(S40),40%精料+20%牛鞭草+40%青贮料.结果表明,S20组平均日增重最高,比CON组提高17.14%(P<0.01),S30组干物质采食量最高,比CON组提高14.51%(P<0.05),S20组肉牛料重比最低(P>0.05).4个处理间干物质、粗蛋白质、中性洗涤纤维和酸性洗涤纤维的表现消化率无显著差异(P>0.05),但是S20组有机物的消化率显著高于CON组(P<0.05).各处理间总蛋白、葡萄糖含量无显著差异(P>0.05),3个试验组尿素氮含量均极显著低于C0N组(P<0.01),分别降低了23.09%、26.10%和30.32%.由此可知,玉米芯-柑橘渣混合青贮料替代部分牛鞭草能提高肉牛生长性能,主要表现在提高肉牛采食量、平均日增重和养分消化率上.玉米芯-柑橘渣混合青贮料的添加量以占饲粮的20%为宜.%This study was conducted to determine the effects of mixed silage with corncob and citrus pulp on growth performance, nutrient digestibility and serum biochemical parameters of beef cattle. A total of 24 healthy Simmental cross cattle with initial body weight of (173.13 ±5.10) kg were randomly allocated into 4 treatments with 6 replicates per treatment and 1 cattle per replicate. The feeding trial lasted for 50 days. Four treatments included 1) control group (CON), 40% basal diet ±60% Hemathria sibirica; 2) test group 1 (S20), 40% basal diet ±40% Hemathria sibirica ±20% mixed silage; 3) test group 2 ( S30), 40% basal diet ±30% Hemathria sibirica ±30% mixed

  2. Effects of Dietary Cation Anion Balance on Blood Biochemical Parameters of Heat Stressed Early Lactation Dairy Cows%阴阳离子平衡对泌乳前期热应激奶牛血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    杨艳玲; 张福寿; 李改英; 高腾云

    2012-01-01

    研究不同水平日粮阴阳离子平衡对泌乳前期热应激奶牛血液生化指标的影响,随机将32头奶牛分为4组,日粮阴阳离子平衡(DCAB)分别为对照185 mEq/kgDM、试验Ⅰ组243 mEq/kgDM、试验Ⅱ组317 mEq/kgDM、试验Ⅲ组442 mEq/kgDM).结果表明:(1)增加DCAB水平可以提高血液的pH值及HCO3 -浓度,降低CO2分压,差异均不显著(P>0.05),唯有试验Ⅲ组TCO2下降显著(P<0.05).(2)随着DCAB水平的增加,血清ALP增加(P>0.05)、血清CK降低(P>0.05);试验Ⅰ组血清TALT高于对照组,其他试验组均低于对照组(P>0.05).(3)试验Ⅱ组血清Na+、K+浓度最高,Na+组间差异显著(P<0.05);血清Cl-浓度随着DCAB的增加而降低,差异不显著(P>0.05);血清Ca2+呈二次曲线变化,差异不显著(P>0.05).(4)试验组T3含量均高于对照组,试验组血清COR、T4含量均低于对照组,试验Ⅰ、Ⅱ组血清PRL含量高于对照组,而试验Ⅲ组则降低,差异均不显著.(5)试验Ⅰ、Ⅱ组血清GLU分别比对照组含量增加,而试验Ⅲ组则显著下降(P<0.05).试验Ⅰ、Ⅱ组血清BUN含量比对照组增加显著(P<0.05),试验Ⅲ组则低于对照组0.78%,其中试验Ⅰ组血清BUN最高.综合DCAB对血液缓冲能力、激素和酶水平等方面考虑,在泌乳前期DCAB为275 meq/kgDM时饲喂效果较好.%To study the effects of DCAB on blood biochemical parameters of heat stressed early lactation dairy cows 32 cows in early lactation were selected respectively, and assigned randomly into 4 groups ( DCAB of the control group was 185 mEq/kgDM, that of the test group I was 243 mEq/kgDM, that of the test group Ewas 317 mEq/kgDM, and that of group HI was 442 mEq/kgDM). The main results showed as follows; ( 1) increasing dietary cation -anion (DCAB) can increase blood pH levels, and HCO3 concentration and reduce the CO2 partial pressure, the difference was not significant (P >0. 05) , TCO2 of group M significantly decreased (P 0.05),serum CK

  3. 大豆肽和乳酸菌素对蛋鸡生产性能、蛋品质及血液生化指标的影响%Effects of Soybean Peptides and Lactobacillin on Performance, Egg Quality and Blood Biochemical Parameters of Laying Hens

    Institute of Scientific and Technical Information of China (English)

    孙汝江; 吕月琴; 张日俊

    2012-01-01

    This experiment was conducted to investigate the effects of dietary soybean peptides (SBP) and Lactobacillin on performance, egg quality and blood biochemical parameter of laying hens. A total of 576 Lo-man laying hens at the "age of 336 days were randomly allotted into 8 groups with 3 replicates per group and 24 hens per replicate. Hens in control group were fed a basal diet and hens in other groups were fed the basal diet supplemented with 0. 075% , 0.15% , 0. 3% SBP, 0. 1% , 0. 2% , 0. 4% Lactobacillin and 0. 15% SBP + 0.2% Lactobacillin, respectively. The adjustment period lasted for 1 week, and the experimental period lasted for 4 weeks. The results showed as follows: 1) adding 0. 15% SBP or 0. 2% Lactobacillin significantly increased laying rate (P >0. 05). 2) Adding 0.15% SBP or 0. 2% Lactobacillin significantly increased the activity of glutamic oxalacetic transaminase (P 0. 05). In conclusion, adding 0.15% SBP or 0. 2% Lactobacillin can improve performance, egg quality and protein metabolism. The effects of adding 0. 15% SBP +0.2% Lactobacillin are better than that of adding SBP or Lactobacillin alone.%本试验旨在研究大豆肽和乳酸菌素对蛋鸡生产性能、蛋品质及血液生化指标的影响.选取336日龄健康罗曼商品蛋鸡576只,随机分成8组,每组3个重复,每个重复24只鸡.对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.075%、0.15%、0.3%大豆肽和0.1%、0.2%、0.4%乳酸菌素及0.15%大豆肽+0.2%乳酸菌素的试验饲粮.预试期1周,正试期4周.结果表明:1)饲粮中添加0.15%大豆肽或0.2%乳酸菌素均可显著提高蛋鸡产蛋率(P<0.01),降低料蛋比(P<0.05),显著提高哈夫单位(P<0.05),对平均日采食量、蛋壳厚度、蛋壳强度无显著影响(P>0.05).2)饲粮中添加0.15%大豆肽或0.2%乳酸菌素可以极显著或显著提高血浆谷草转氨酶(GOT) (P <0.01,P<0.05)、谷丙转氨酶(GPT)活性(P<0.01),

  4. 温度和饲料蛋白质水平对松浦镜鲤幼鱼血清生化指标的影响%Effects of temperature and dietary protein content on the blood biochemical parameters in juvenile Songpu mirror carp Cyprinus specularis Songpu

    Institute of Scientific and Technical Information of China (English)

    黄金凤; 徐奇友; 王常安; 赵志刚; 罗亮

    2013-01-01

    The effects of water temperature (18, 23 ℃, and 28 ℃ ) and dietary protein levels (30% , 32% , 34% , 36% and 38%) on blood biochemical parameters were investigated in juvenile (body weight 10. 11 g±l. 07 g) Songpu mirror carp Cyprinus specularis Songpu reared in recirculating tanks at a rate of 30 individuals per tank with triplication for 60 d. The results showed that total protein (TP) and triglyceride (TG) levels in the juveniles were increased with the increase in dietary protein levels at 18 ℃, while glutamate-oxaloacetate transaminase (AST) activity decreased with the increase in dietary protein levels at 18 ℃. There were no significant differences in total cholesterol (CHOL) levels at all temperature (P>0.05) , and urea nitrogen (BUN), creatinine (CREA), blood glucose (GLU) , and albumin (ALB) levels, and glutamate-pyruvate transaminase (ALT) activity, and athero-genic lipoprotein profile (ALP) levels were increased then decreased with increase in dietary protein levels. There were no significant differences (P>0.05) in protein treatments. Under the same protein level, TG, BUN, CREA, TP, ALB, ALT and AST levels were significant decreased (P<0.05) with the increase in temperature, while ALP level were significant increased(P<0.05). At 23 ℃ , GLU and GGT levels were significant higher than that in the other temperature treatments. There was better nutritive metabolism of Songou mirror carp at 23 ℃ than at 18 and 28 X ,and the optimal dietary protein level was found to be 32% at 18 ℃ , 34% at 23 ℃ and 34% at 28 ℃.%研究了在不同温度(18、23、28℃)下,饲喂5种蛋白质水平(质量分数为30%、32%、34%、36%和38%)的饲料对松浦镜鲤Cyprinus specularis Songpu幼鱼(10.11 g±1.07 g)血清生化指标的影响.试验分为15组,每组设3个重复,每个重复放30尾鱼,饲养试验共进行60 d.结果表明:18℃下,鱼血清总蛋白(TP)和甘油三酯(TG)含量随饲料蛋白质水平的升高而升高,血清谷

  5. Biochemical response of amphipods (Gammarid: Paramorea) in a sediment laboratory exposure from Ushuaia Bay, Beagle Channel.

    Science.gov (United States)

    Schvezov, Natasha; Amin, Oscar

    2011-03-01

    A coastal system (Ushuaia Bay, Argentina) impacted by anthropogenic activities was studied by the response of local amphipods (Parmorea sp., Gammaridae) to the exposure of coastal sediments in a laboratory assay. Four coastal areas with different loadings of contaminants and one considered as reference were studied. Organic matter, carbohydrates, proteins and heavy metals were measured in sediment samples. Organisms were exposed to sediments for seven days and catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) and lipid peroxidation (LPO) were measured afterward. Amphipods exhibited an activation of GST and inhibition of AChE in most impacted areas. Principal Component Analysis (PCA) was conducted in order to associate the biological responses with sediment metal concentration and its eutrophicated status. Levels of Cd and Cr were associated with the inhibition of AChE and with the enhancement of GST. CAT and LPO were enhanced in most areas, but no link was found with the contaminants studied by PCA, suggesting that other parameters present in sediments not included in the PCA affect the amphipods. The most impacted area corresponds to Nautical Club station, with a highly eutrophicated status and high content of metals, where amphipods after the exposure were affected in a biochemical level. PMID:21239059

  6. Radiation and cadmium induced biochemical alterations in mouse kidney

    International Nuclear Information System (INIS)

    In the present investigation radiation and cadmium induced biochemical changes in the kidney of Swiss albino mice have been studied. Materials and Methods: For this purpose, adult male Swiss albino mice (6-8 weeks old) were divided into four groups. Group I (sham-irradiated), Group II (treated with CdCl2 solution 20 ppm), Group III (irradiated with 1.25, 2.5 and 5.0 Gy gamma rays), Group IV (both irradiated with 1.25, 2.5 and 5.0 Gy gamma rays and treated with CdCl2 solution). The animals were autopsied after 1, 2, 4, 7, 14 and 28 days of treatment. The kidney was taken out and different biochemical parameters, such as total proteins, glycogen, cholesterol, acid phosphatase activity, alkaline phosphatase activity, DNA and RNA were estimated. Results: In irradiated animals, the values of total proteins, glycogen, acid phosphatase, alkaline phosphatase activity and RNA increased continuously up to day-7 and decreased thereafter up to day-28. The changes were dose dependent. In CdCl2 treated animals, the values of glycogen and total proteins decreased during the early intervals and increased thereafter whereas the values of acid and alkaline phosphatase activity and RNA increased during early Intervals and decreased thereafter, The values of cholesterol and DNA showed decrease in all the experimental groups (except group I) up to day-7 and increase thereafter up to day-28. After combined treatment also, the parameters followed the same pattern of increase and decrease, but the changes were more pronounced indicating their synergistic effect. The biochemical parameters showed highly significant values (P<0.001) as compared to normal ones. Conclusion: These results indicate that combined treatment of cadmium and gamma radiations causes synergistic or additive effect

  7. BIOCHEMICAL NUTRITIONAL PROFILE OF LIVER CIRRHOSIS PATIENTS WITH HEPATOCELLULAR CARCINOMA

    Directory of Open Access Journals (Sweden)

    Gabriela Zanatta PORT

    2014-03-01

    Full Text Available Context Liver cirrhosis patients with hepatocellular carcinoma present nutritional alterations and metabolic disorders that negatively impact the prognosis. Objective The objective is to identify alterations in the metabolism of macro and micronutrients among liver cirrhosis patients with and without hepatocellular carcinoma and their relation to the Child-Turcote-Pugh score and Barcelona Clinic Liver Cancer staging. Methods Analytical transversal study, with 31 hepatocellular carcinoma patients and 48 liver cirrhosis patients. Laboratorial exams were carried out. The existence of an association between the biochemical parameters and the disease severity as well as the presence of hepatocellular carcinoma was assessed. Results The metabolic-nutritional profile of liver cirrhosis patients caused by the hepatitis C virus and hepatocellular carcinoma showed alterations, specifically the lipid (total cholesterol, HDL and triglycerides, protein (albumin, creatinine and uric acid, iron (transferrin, iron and ferritin saturation, hematocrit and hemoglobin, zinc and B12 vitamin profiles. There is a relation between nutritional biochemical markers and the Child-Turcote-Pugh, as well as Barcelona Clinic Liver Cancer staging. Conclusions Considering the existence of alterations in the metabolism of nutrients in liver cirrhosis patients with and without hepatocellular carcinoma, and also that conventional nutritional assessment methods present limitations for this population, the biochemical laboratorial exams are valid to complement the diagnosis of the nutritional state in a quick and practical manner.

  8. Biochemical Characterization of Hypothetical Proteins from Helicobacter pylori.

    Directory of Open Access Journals (Sweden)

    Han-Pil Choi

    Full Text Available The functional characterization of Open Reading Frames (ORFs from sequenced genomes remains a bottleneck in our effort to understand microbial biology. In particular, the functional characterization of proteins with only remote sequence homology to known proteins can be challenging, as there may be few clues to guide initial experiments. Affinity enrichment of proteins from cell lysates, and a global perspective of protein function as provided by COMBREX, affords an approach to this problem. We present here the biochemical analysis of six proteins from Helicobacter pylori ATCC 26695, a focus organism in COMBREX. Initial hypotheses were based upon affinity capture of proteins from total cellular lysate using derivatized nano-particles, and subsequent identification by mass spectrometry. Candidate genes encoding these proteins were cloned and expressed in Escherichia coli, and the recombinant proteins were purified and characterized biochemically and their biochemical parameters compared with the native ones. These proteins include a guanosine triphosphate (GTP cyclohydrolase (HP0959, an ATPase (HP1079, an adenosine deaminase (HP0267, a phosphodiesterase (HP1042, an aminopeptidase (HP1037, and new substrates were characterized for a peptidoglycan deacetylase (HP0310. Generally, characterized enzymes were active at acidic to neutral pH (4.0-7.5 with temperature optima ranging from 35 to 55°C, although some exhibited outstanding characteristics.

  9. Evaluation of texture parameters for the quantitative description of multimodal nonlinear optical images from atherosclerotic rabbit arteries

    International Nuclear Information System (INIS)

    The composition and structure of atherosclerotic lesions can be directly related to the risk they pose to the patient. Multimodal nonlinear optical (NLO) microscopy provides a powerful means to visualize the major extracellular components of the plaque that critically determine its structure. Textural features extracted from NLO images were investigated for their utility in providing quantitative descriptors of structural and compositional changes associated with plaque development. Ten texture parameters derived from the image histogram and gray level co-occurrence matrix were examined that highlight specific structural and compositional motifs that distinguish early and late stage plaques. Tonal-texture parameters could be linked to key histological features that characterize vulnerable plaque: the thickness and density of the fibrous cap, size of the atheroma, and the level of inflammation indicated through lipid deposition. Tonal and texture parameters from NLO images provide objective metrics that correspond to structural and biochemical changes that occur within the vessel wall in early and late stage atherosclerosis.

  10. Evaluation of texture parameters for the quantitative description of multimodal nonlinear optical images from atherosclerotic rabbit arteries

    Energy Technology Data Exchange (ETDEWEB)

    Mostaco-Guidolin, Leila B; Ko, Alex C-T; Popescu, Dan P; Smith, Michael S D; Kohlenberg, Elicia K; Sowa, Michael G [Institute for Biodiagnostics, National Research Council Canada, Winnipeg, R3B 1Y6 (Canada); Shiomi, Masashi [Institute of Experimental Animals, School of Medicine, Kobe University, Kobe 650-0017 (Japan); Major, Arkady [Department Electrical and Computer Engineering, University of Manitoba, E3-559 Engineering Building, Winnipeg, R3T 5V6 (Canada)

    2011-08-21

    The composition and structure of atherosclerotic lesions can be directly related to the risk they pose to the patient. Multimodal nonlinear optical (NLO) microscopy provides a powerful means to visualize the major extracellular components of the plaque that critically determine its structure. Textural features extracted from NLO images were investigated for their utility in providing quantitative descriptors of structural and compositional changes associated with plaque development. Ten texture parameters derived from the image histogram and gray level co-occurrence matrix were examined that highlight specific structural and compositional motifs that distinguish early and late stage plaques. Tonal-texture parameters could be linked to key histological features that characterize vulnerable plaque: the thickness and density of the fibrous cap, size of the atheroma, and the level of inflammation indicated through lipid deposition. Tonal and texture parameters from NLO images provide objective metrics that correspond to structural and biochemical changes that occur within the vessel wall in early and late stage atherosclerosis.

  11. BIOCHEMICAL SCREENING OF DIABETIC NEPHROPATHY

    Directory of Open Access Journals (Sweden)

    Vivek

    2016-01-01

    Full Text Available Diabetic nephropathy is a clinical syndrome characterized by the following- Persistent albuminuria (>300mg/d or >200μg/min, that is confirmed on at least 2 occasions 3-6 months apart diabetic, progressive decline in the Glomerular Filtration Rate (GFR, elevated arterial blood pressure. The earliest biochemical criteria for the diagnosis of diabetic nephropathy is the presence of micro-albumin in the urine, which if left untreated will eventually lead to End-Stage Renal Disease (ESRD. Micro-albuminuria refers to the excretion of albumin in the urine at a rate that exceeds normal limits. The current study was conducted to establish the prevalence of micro-albuminuria in a sequential sample of diabetic patients attending hospital and OPD Clinic to determine its relationship with known and putative risk factors to identify micro- and normo-albuminuric patients in their sample for subsequent comparison in different age, sex, weight and creatinine clearance of the micro- and normo-albuminuric patients. This cross-sectional analytical study was conducted in one hundred patients at Saraswathi Institute of Medical Sciences, Anwarpur, Hapur, U. P. Patients having diabetes mellitus in different age group ranging from 30 to 70 years were selected. Data was analysed by SPSS software. Micro-albuminuria was observed in 35% in patients with type 2 diabetes mellitus. It was observed that 65% patients were free from any type of albuminuria. Also micro-albuminuria was present in 10% of the patients less than 50 yrs. of age, while 15% of the patients more than 50 yrs. of age were having micro-albuminuria. There was a statistically significant correlation of micro-albuminuria with duration of diabetes. Incidence of micro-albuminuria increases with age as well as increased duration of diabetes mellitus. Our study shows that only 5% patients developed macro-albuminuria. Glycosylated haemoglobin and fasting plasma glucose was significantly raised among all these

  12. Spatio-temporal patterns with hyperchaotic dynamics in diffusively coupled biochemical oscillators

    Directory of Open Access Journals (Sweden)

    Gerold Baier

    1997-01-01

    Full Text Available We present three examples how complex spatio-temporal patterns can be linked to hyperchaotic attractors in dynamical systems consisting of nonlinear biochemical oscillators coupled linearly with diffusion terms. The systems involved are: (a a two-variable oscillator with two consecutive autocatalytic reactions derived from the Lotka–Volterra scheme; (b a minimal two-variable oscillator with one first-order autocatalytic reaction; (c a three-variable oscillator with first-order feedback lacking autocatalysis. The dynamics of a finite number of coupled biochemical oscillators may account for complex patterns in compartmentalized living systems like cells or tissue, and may be tested experimentally in coupled microreactors.

  13. Diurnal changes of biochemical metabolic markers in healthy young males

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Fahrenkrug, Jan

    2015-01-01

    .06 mmol/L) did not show significant oscillations. CONCLUSIONS: When diagnosing and monitoring metabolic disorders compensation for the 24-h variation of the biochemical metabolic markers is needed especially C-peptide, triglyceride and glucose. Furthermore, the stable HbA1c level through 24 h makes......BACKGROUND: To examine whether time of the day has an effect on the circulating levels of metabolism parameters. METHODS: Venous blood samples were obtained under standardized conditions from 24 healthy young men every third hour through 24 hours. The metabolic markers and melatonin were examined...

  14. Interferência do ácido ascórbico nas determinações de parâmetros bioquímicos séricos: estudos in vivo e in vitro Ascorbic acid interference on the measurement of serum biochemical parameters: in vivo and in vitro studies

    Directory of Open Access Journals (Sweden)

    Flávia Martinello

    2003-01-01

    Full Text Available INTRODUÇÃO: O ácido ascórbico, quando presente em amostras biológicas, pode interferir nos ensaios laboratoriais que utilizam reações de oxidorredução. OBJETIVOS: Estabelecer o grau de interferência do ácido ascórbico nas determinações bioquímicas séricas em relação à dose de vitamina C, ingerida e ao tempo de coleta das amostras e comparar com o efeito interferente in vitro. MÉTODOS: Voluntários saudáveis (n = 18 consumiram doses crescentes e sucessivas de 0,25 a 4g/dia de vitamina C durante uma semana cada dose. As determinações bioquímicas de ácido úrico, bilirrubina, colesterol total, glicose e triglicerídeos foram realizadas antes e 4, 12 e 24 horas após a última ingestão de cada dose. No estudo in vitro, concentrações crescentes de ácido ascórbico foram adicionadas ao soro humano e os analitos foram determinados até 24 horas após a adição. RESULTADOS E DISCUSSÃO: Os níveis séricos de ácido ascórbico aumentaram significativamente após a ingestão da vitamina C, provocando inibição nas determinações de ácido úrico e bilirrubina total 4, 12 e 24 horas após a ingestão (p BACKGROUND: Ascorbic acid, when present in biological samples, can produce a negative interference in several biochemical tests that use redox indicator systems. OBJECTIVES: We evaluated the ascorbic acid interference on serum biochemical tests in relation to the dose of vitamin C ingested and to the time of blood collection, and compared these results with in vitro interference. METHODS: Healthy volunteers (n =18 consumed increasing and successive doses of vitamin C (0.25 to 4g/day for one week. Biochemical analytes were measured before and after each dose consumption. Serum samples were obtained 4, 12 and 24 h after vitamin C ingestion. Ascorbic acid was added in vitro to the human serum and the biochemical analytes were assayed up to 24 h after addition. RESULTS AND DISCUSSION: Serum ascorbic acid increased significantly

  15. 饲粮蛋白脂肪比对圆斑星鲽(Verasper variegates)生长、消化酶及血清生化指标的影响%The Effects of the Ratio of Dietary Protein to Lipid on the Growth, Digestive Enzyme Activities and Blood Biochemical Parameters in Spotted Halibut,Verasper variegates

    Institute of Scientific and Technical Information of China (English)

    吕云云; 陈四清; 于朝磊; 常青; 秦搏; 王志军

    2015-01-01

    Spotted halibut,Verasper variegates has been a target model in the studies of reproductive biology and molecular biology. However, litter is known about the dietary requirements of this species. In this study, we determined the optimal ratio of protein to lipid for this fish and analyzed the combined effects on the growth,digestive enzyme activities and blood biochemical parameters inspotted halibut. Fish meal and casein were used as protein sources, and soybean oil and fish oil served as lipid sources. Nine experimental diets were formulated as combinations of three levels of crude proteins (40%, 45%, and 50%) and three levels of crude lipid (8%, 12%, and 16%), namely P40L8, P40L12, P40L16, P45L8, P45L12, P45L16, P50L8, P50L12 and P50L16. They were randomly distributed into 27 cement ponds (2 m×1 m×1 m) with 20 fish/pond for 83 d in an in-door flow-through aquarium system. The results showed that the survival rate of fish was not significantly affected by the levels of protein and lipid (P>0.05). The weight gain, specific growth rate and feed intake were significantly reduced along with the increase in the dietary lipid (P<0.05). Compared to P40L16, the weight gain in P50L8 was 33.63% higher and the specific growth rate was 31% higher. The feed efficiency was significantly boosted along with the increase in the dietary protein (P<0.05). The feed efficiency in P50L8 was 24.09% higher than P40L8, and the weight gain, specific growth rate, feed intake and feed efficiency in P50L8 were significantly higher than those in other groups. The activity of stomach protease was first increased and then decreased along with the increase in dietary protein. The protease activity in the hepatopancreas in P50L8 group was significantly higher than that in P50L12 group (P<0.05), but showed no significant differences with other groups (P>0.05). The activity of the stomach lipase was first decreased and then increased along with the increase in the dietary protein. The

  16. The Effects of the Ratio of Dietary Protein to Lipid on the Growth, Digestive Enzyme Activities and Blood Biochemical Parameters in Spotted Halibut,Verasper variegates%饲粮蛋白脂肪比对圆斑星鲽(Verasper variegates)生长、消化酶及血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    吕云云; 陈四清; 于朝磊; 常青; 秦搏; 王志军

    2015-01-01

    Spotted halibut,Verasper variegates has been a target model in the studies of reproductive biology and molecular biology. However, litter is known about the dietary requirements of this species. In this study, we determined the optimal ratio of protein to lipid for this fish and analyzed the combined effects on the growth,digestive enzyme activities and blood biochemical parameters inspotted halibut. Fish meal and casein were used as protein sources, and soybean oil and fish oil served as lipid sources. Nine experimental diets were formulated as combinations of three levels of crude proteins (40%, 45%, and 50%) and three levels of crude lipid (8%, 12%, and 16%), namely P40L8, P40L12, P40L16, P45L8, P45L12, P45L16, P50L8, P50L12 and P50L16. They were randomly distributed into 27 cement ponds (2 m×1 m×1 m) with 20 fish/pond for 83 d in an in-door flow-through aquarium system. The results showed that the survival rate of fish was not significantly affected by the levels of protein and lipid (P>0.05). The weight gain, specific growth rate and feed intake were significantly reduced along with the increase in the dietary lipid (P<0.05). Compared to P40L16, the weight gain in P50L8 was 33.63% higher and the specific growth rate was 31% higher. The feed efficiency was significantly boosted along with the increase in the dietary protein (P<0.05). The feed efficiency in P50L8 was 24.09% higher than P40L8, and the weight gain, specific growth rate, feed intake and feed efficiency in P50L8 were significantly higher than those in other groups. The activity of stomach protease was first increased and then decreased along with the increase in dietary protein. The protease activity in the hepatopancreas in P50L8 group was significantly higher than that in P50L12 group (P<0.05), but showed no significant differences with other groups (P>0.05). The activity of the stomach lipase was first decreased and then increased along with the increase in the dietary protein. The

  17. Biochemical genetics of some Indian fishes

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.; Qasim, S.Z.

    Studies on biochemical genetics of fishes, using electrophoretic methods, are relatively of recent origin. Earlier serum and eye lens protein were used to identify marine populations. This technique showed that closely related species have...

  18. Physics and Electro-Biochemical Technology

    Directory of Open Access Journals (Sweden)

    Conrad P Pritscher

    2008-12-01

    Full Text Available Not Available Keywords: Biochemical technology, physics Received: 22 October 2008 / Received in revised form: 23 October 2008, Accepted: 24 October 2008 Published online: 07 January 2009

  19. Free energy simulations of important biochemical processes

    OpenAIRE

    Yang LIU; 刘洋

    2013-01-01

    Free energy simulations have been widely employed to compute the thermodynamic properties of many important biochemical processes. In the first part of this dissertation, two important biochemical processes, protonation/deprotonation of acid in solution and solvation of small organic molecules, are investigated using free energy simulations. Accurate computation of the pKa value of a compound in solution is important and challenging. To efficiently simulate the free energy change associat...

  20. Bioimpedance spectroscopy as technique of hematological and biochemical analysis of blood

    Science.gov (United States)

    Malahov, M. V.; Smirnov, A. V.; Nikolaev, D. V.; Melnikov, A. A.; Vikulov, A. D.

    2010-04-01

    Bioimpedance spectroscopy may become a useful method for the express analysis and monitoring of blood parameters. The aim of this study was to identify biochemical and hematological parameters of blood that can be accurately predicted by means of bioimpedance technique. Hematological (red blood cell and white blood cell parameters) and biochemical (total proteins, albumins, fibrinogen, sodium, potassium, chloride ion concentrations in plasma) parameters were measured with a hematological analyzer and routine methods. Bioimpedance spectroscopy of the whole blood (1.5 ml) in frequency range 5-500 kHz (31 frequencies) was performed using BIA analyzer ABC-01 "Medass". Frequency relationships of resistance and reactance of the whole blood and the parameters of the Cole model were investigated. Close simple and multiple correlations of bioimpedance indices were observed only with erythrocyte parameters (Ht, Hb, RBC). Thus bioimpedance analysis of the whole blood can accurately predict red cell parameters but it is less effective for estimation of plasma biochemical and white cell parameters.

  1. Haematological and biochemical alterations caused by epidural and intramuscular administration of xylazine hydrochloride in dromedary camels (Camelus dromedarius

    Directory of Open Access Journals (Sweden)

    Omid Azari

    2012-09-01

    Full Text Available This study was conducted in 16 healthy immature dromedary camels weighing 120-150 kg to evaluate and compare the effects of epidural and intramuscular injections of xylazine administered at 0.1 mg/kg and 0.2 mg/kg. Haematological parameters included haemoglobin, packed cell volume, total erythrocyte count and total leukocyte count. Biochemical parameters included alkaline phosphates, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine and glucose. Parameters were measured at different intervals before (baseline and after the administration of drugs. Our study showed that the effect of xylazine on haematological and biochemical parameters is dose-dependant and is also related to the route of administration. The low dose of xylazine administered using both intramuscular and epidural methods showed minimal effects, whereas high doses of the drug, especially when injected intramuscularly, caused greater changes in haematological and biochemical parameters.

  2. Linked data management

    CERN Document Server

    Hose, Katja; Schenkel, Ralf

    2014-01-01

    Linked Data Management presents techniques for querying and managing Linked Data that is available on today’s Web. The book shows how the abundance of Linked Data can serve as fertile ground for research and commercial applications. The text focuses on aspects of managing large-scale collections of Linked Data. It offers a detailed introduction to Linked Data and related standards, including the main principles distinguishing Linked Data from standard database technology. Chapters also describe how to generate links between datasets and explain the overall architecture of data integration systems based on Linked Data. A large part of the text is devoted to query processing in different setups. After presenting methods to publish relational data as Linked Data and efficient centralized processing, the book explores lookup-based, distributed, and parallel solutions. It then addresses advanced topics, such as reasoning, and discusses work related to read-write Linked Data for system interoperation. Desp...

  3. Spamming in Linked Data

    OpenAIRE

    Hasnain, Ali; Al-Bakri, Mustafa; Costabello, Luca; Cong, Zijie; Davis, Ian; Heath, Tom

    2012-01-01

    The rapidly growing commercial interest in Linked Data raises the prospect of "Linked Data spam", which we define as "deliberately misleading information (data and links) published as Linked Data, with the goal of creating financial gain for the publisher". Compared to conventional technologies affected by spamming, e.g. email and blogs, spammers targeting Linked Data may not be able to push information directly towards consumers, but rather may seek to exploit a lack of human involvement in ...

  4. Linking numbers for handlebody-links

    OpenAIRE

    Mizusawa, Atsuhiko

    2013-01-01

    As a generalization of the linking number, we construct a set of invariant numbers for two-component handlebody-links. These numbers are elementary divisors associated with the natural homomorphism from the first homology group of a component to that of the complement of another component.

  5. CORD SERUM FERRITIN AS BIOCHEMICAL MARKER IN IRON DEFICIENCY ANEMIA

    Directory of Open Access Journals (Sweden)

    Sherin

    2014-07-01

    Full Text Available Iron deficiency anemia is by far the most frequent type of anemia seen in pregnancy, accounting for 90% or more of all cases. Iron deficiency anemia has adverse consequences on infant development. Therefore maternal anemia should be prevented and treated. Serum ferritin is the single best non-invasive test and is a very useful and reliable index of iron stores especially during pregnancy, with low levels indicating iron deficiency. While infants born to anemic mother are themselves not anemic, they do not suffer from low iron stores. The main aim of the study is to establish cord serum ferritin as a biochemical marker in iron deficiency anemia. The specific objectives of this study is to estimate cord and maternal serum ferritin in the last trimester of pregnancy and to correlate it with hematological parameters. Cord serum ferritin levels were normal in the patients under study, even when the maternal serum ferritin levels were decreased. The mean level of cord serum ferritin was 134.06ng/ml and maternal serum ferritin was 41.65ng/ml and was statistically significant. The hematological parameters like hemoglobin, hematocrit, MCV and MCHC were also decreased in the patients with low serum ferritin and were found to be statistically significant. Hence estimation of cord serum ferritin can be used as a biochemical marker to assess iron deficiency in the early stages itself and thereby neurodevelopmental complications in children can be prevented.

  6. Human biochemical response to ozone and vitamin E

    Energy Technology Data Exchange (ETDEWEB)

    Posin, C.I.; Clark, K.W.; Jones, M.P.; Buckley, R.D.; Hackney, J.D.

    1979-11-01

    To determine whether vitamin E (dl-..cap alpha..-tocopherol) supplementation of the diet provides protection from inhaled oxidants such as ozone (O/sub 3/) in community air pollution, its effects were studied in healthy adult volunteers. Experimental groups received 800 or 1600 IU of vitamin E for 9 weeks or more; control groups received placebos. Double-blind conditions were maintained throughout the study. Biochemical parameters studied included red blood cell fragility; hematocrit and hemoglobin values; red cell glutathione concentration; and the enzymes acetylcholinesterase, glucose-6-phosphate dehydrogenase, and lactic acid dehydrogenase. No significant differences between the responses of the supplemented and placebo groups to a controlled O/sub 3/ exposure (0.5 ppM for 2 h) were found for any of these parameters. The results indicate that vitamin E supplementation in humans, at the levels employed in this experiment, gives no added protection against blood biochemical effects of O/sub 3/ in intermittently exercising subjects under exposure conditions simulating summer ambient air pollution episodes.

  7. Transient amplification limits noise suppression in biochemical networks

    Science.gov (United States)

    Dixon, John; Lindemann, Anika; McCoy, Jonathan H.

    2016-01-01

    Cell physiology is orchestrated, on a molecular level, through complex networks of biochemical reactions. The propagation of random fluctuations through these networks can significantly impact cell behavior, raising challenging questions about how network design shapes the cell's ability to suppress or exploit these fluctuations. Here, drawing on insights from statistical physics, fluid dynamics, and systems biology, we explore how transient amplification phenomena arising from network connectivity naturally limit a biochemical system's ability to suppress small fluctuations around steady-state behaviors. We find that even a simple system consisting of two variables linked by a single interaction is capable of amplifying small fluctuations orders of magnitude beyond the levels predicted by linear stability theory. We also find that adding additional interactions can promote further amplification, even when these interactions implement classic design strategies known to suppress fluctuations. These results establish that transient amplification is an essential factor determining baseline noise levels in stable intracellular networks. Significantly, our analysis is not bound to specific systems or interaction mechanisms: we find that noise amplification is an emergent phenomenon found near steady states in any network containing sufficiently strong interactions, regardless of its form or function.

  8. Evaluating biochemical methane production from brewer's spent yeast.

    Science.gov (United States)

    Sosa-Hernández, Ornella; Parameswaran, Prathap; Alemán-Nava, Gibrán Sidney; Torres, César I; Parra-Saldívar, Roberto

    2016-09-01

    Anaerobic digestion treatment of brewer's spent yeast (SY) is a viable option for bioenergy capture. The biochemical methane potential (BMP) assay was performed with three different samples (SY1, SY2, and SY3) and SY1 dilutions (75, 50, and 25 % on a v/v basis). Gompertz-equation parameters denoted slow degradability of SY1 with methane production rates of 14.59-4.63 mL/day and lag phases of 10.72-19.7 days. Performance and kinetic parameters were obtained with the Gompertz equation and the first-order hydrolysis model with SY2 and SY3 diluted 25 % and SY1 50 %. A SY2 25 % gave a 17 % of TCOD conversion to methane as well as shorter lag phase (methane production. Methane capture and biogas composition were dependent upon the SY source, and co-digestion (or dilution) can be advantageous. PMID:27276935

  9. 40 CFR 158.2010 - Biochemical pesticides data requirements.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Biochemical pesticides data...) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Biochemical Pesticides § 158.2010 Biochemical pesticides... required to support registration of biochemical pesticides. Sections 158.2080 through 158.2084 identify...

  10. 40 CFR 158.2000 - Biochemical pesticides definition and applicability.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Biochemical pesticides definition and...) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Biochemical Pesticides § 158.2000 Biochemical pesticides definition and applicability. This subpart applies to all biochemical pesticides as defined in paragraphs...

  11. MICROBIOLOGIC AND BIOCHEMICAL ATTRIBUTES AS INDICATORS OF THE RECOVERY OF DEGRADED AREAS, IN ITAJUBÁ/MG

    Directory of Open Access Journals (Sweden)

    Rafael Botelho Silveira

    2006-03-01

    Full Text Available This paper evaluated and compared the microbial and biochemical parameters of degraded areas, in rehabilitation,with natural areas, considered reference, without recent human interference, in Itajubá (MG, Brazil, to verify their capacity to indicatethe soil quality and rehabilitation capacity. Soil samples were collected in February 2003 (summer, on six sites: three in natural areasand other three in degraded areas, under rehabilitation. Total density of fungi and bacteria, phosphate solubilizing microorganisms,microbial biomass C and activity were evaluated. Moreover were established relationships among visual and microbial and biochemicalparameters, verifying their potential use as indicators in studies of soil rehabilitation on degraded areas. Degradation promotedstrong negative impact in the soil microbiota, reducing the number of all microbial groups studied, microbial activity and biomass.The soil microbial and biochemical parameters were considered good indicators of the soil rehabilitation on degraded areas. The soilrehabilitation evidenced by visual parameters of soil, plants and organisms had not direct positive relationship with those observedin the underground through microbial and biochemical indicators. These findings show that rehabilitation of degraded areas isstrongly affected by soil microbial and biochemical parameters.

  12. High-resolution mapping of bifurcations in nonlinear biochemical circuits

    Science.gov (United States)

    Genot, A. J.; Baccouche, A.; Sieskind, R.; Aubert-Kato, N.; Bredeche, N.; Bartolo, J. F.; Taly, V.; Fujii, T.; Rondelez, Y.

    2016-08-01

    Analog molecular circuits can exploit the nonlinear nature of biochemical reaction networks to compute low-precision outputs with fewer resources than digital circuits. This analog computation is similar to that employed by gene-regulation networks. Although digital systems have a tractable link between structure and function, the nonlinear and continuous nature of analog circuits yields an intricate functional landscape, which makes their design counter-intuitive, their characterization laborious and their analysis delicate. Here, using droplet-based microfluidics, we map with high resolution and dimensionality the bifurcation diagrams of two synthetic, out-of-equilibrium and nonlinear programs: a bistable DNA switch and a predator–prey DNA oscillator. The diagrams delineate where function is optimal, dynamics bifurcates and models fail. Inverse problem solving on these large-scale data sets indicates interference from enzymatic coupling. Additionally, data mining exposes the presence of rare, stochastically bursting oscillators near deterministic bifurcations.

  13. Bomb parameters

    International Nuclear Information System (INIS)

    The reconstruction of neutron and gamma-ray doses at Hiroshima and Nagasaki begins with a determination of the parameters describing the explosion. The calculations of the air transported radiation fields and survivor doses from the Hiroshima and Nagasaki bombs require knowledge of a variety of parameters related to the explosions. These various parameters include the heading of the b