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Sample records for biochemical parameters concentration

  1. The Effects of Air Pollution and Smoking on Cadmium Concentration in Human Blood and Correlation with Biochemical Parameters

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    L. Zeneli

    2009-01-01

    Full Text Available Problem statement: The study described the research of the effects that the environment pollution and smoking have in cadmium concentration in human blood, as well as in the correlation between cadmium and the biochemical parameters. Approach: In a comparative study of cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants (Obiliq, a highly polluted environments (Investigated Group and the other that was considered as relatively clean rural environment Dragash (control group. Results: The results showed that there exists a significant difference in the average concentration of cadmium in human blood between the Investigated Group (IG and the Control Group (CG (t = -3.34, p = 0.0006. The series of determination of cadmium concentration in blood of population that lives in this environment had shown direct effects in biochemical parameters (direct bilirubine, total bilirubine. Conclusion: Air pollution (from coal burning in power plant and smoking were very important factors for the level of cadmium concentration in blood, which had an inhibitory effect in the syntheses of bilirubine.

  2. Haematological and serum biochemical parameters of West African Dwarf goats fed dried cassava leaves-based concentrate diets.

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    Oni, Adebayo Olusoji; Arigbede, Oluwasanmi Moses; Sowande, Olusiji Sunday; Anele, Uchenna Young; Oni, Oluwakemi Oluremilekun; Onwuka, Chryss Friday Ijeoma; Onifade, Olufemi Sunday; Yusuf, Kafayat Omowumi; Dele, Peter Aniwe; Aderinboye, Ronke Yemisi

    2012-03-01

    The study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of West African Dwarf (WAD) goats. The study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. At the start of the experiment, packed cell volume (PCV) ranged from 21.5% to 25.5% while haemoglobin concentration (Hb) and RBC significantly (P cassava leaves increased in the diets. At the end of the trial, there was a slight increase in the values of PCV and Hb in the diets (P > 0.05). Lymphocyte reduced significantly (P  0.05) at the 0% to 40% levels and reduced at the 60% level of dried cassava leaves inclusion. At the start of the experiment, values for glucose significantly (P cassava leaves increased from 0% to 60% in the diets. The study revealed that inclusion of dried cassava leaves in the diets of West African Dwarf goats had no deleterious effects on the haematological and serum biochemical parameters of WAD goats and could therefore be included in ruminant diets up to 60%.

  3. Effects of pollution on lead and cadmium concentration and correlation with biochemical parameters in blood of human population nearby Kosovo thermo power plants

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    L. Zeneli

    2008-01-01

    Full Text Available This study describes an investigation of lead and cadmium pollution of Kosovo environment as a result of outflow from the coal processing industry. In a comparative study of lead and cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants, (Obiliq a highly polluted environment and the other that is considered as relatively clean rural environment (Dragash. Analysis has shown that emission of particulate in fly ash from Thermo Power Plants during 2005 has exceeded EU standards by 400-500% and that lead concentration was 18mg kg-1 and cadmium concentration was -1 of ash. A series of determinations of lead and cadmium concentrations in blood of population that lives in this environment, have shown direct effects in biochemical parameters CRE (Creatinin, DB (Direct Bilirubine, TB (Total Bilirubine, AST (Aspartat Aminotransferaza, CK (Creatin Kinaza and CHE (Cholenisteraza in human organism. The results that were achieved in this study showed a significant difference in average lead and cadmium concentration in the blood of the investigated group of peoples that lives in the area near by the Power Plants, from a control group that lives in a rural unpolluted environment. Lead and cadmium has been analyzed in 50 samples taken from persons from industrial zone and 25 samples in controlled group. The level of lead concentration was 23.0-112.1 µg L-1in geometric average 46.05 µg L-1, cadmium concentration was 0.44-6.02 µg L-1 in geometric average of 1.56µg L-1. Controlled group from the rural relatively clean environment showed lead concentration of 6.7-33.8 µg L-1 in geometric avarage 17.76 µg L-1 and cadmium concentration of 0.21-1.8 µg L-1 or in geometric average of 0.73 µg L-1. In conclusion in exposed subjects, pollution from coal burning in Power Plant is very important factor for level of lead and cadmium concentration in blood of tested population.

  4. Effects of iron-glycine chelate on growth, carcass characteristic, liver mineral concentrations and haematological and biochemical blood parameters in broilers.

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    Kwiecień, M; Samolińska, W; Bujanowicz-Haraś, B

    2015-12-01

    Studies were carried out to determine the effect of additive iron-glycine chelate on the production performance, slaughter yield, mineral deposition in the liver and the metabolic blood panel in broiler chickens. A total of 250 one-day-old Ross 308 chicks were allotted into five groups with five replicates of 10 birds each. Diets were supplemented with the organic form iron (Fe-Gly at the rate of 25%, 50% or 100% of the total requirements of the elements) and inorganic Fe (FeSO4 at the rate of 50% or 100%). In the experiment, iron was added to the premix (containing no Fe) in an amount of 40 or 20 mg per kg of basal diet, in groups I and II, in the form of FeSO4 , and in an amount of 40, 20 or 10 mg per kg of basal diet, in groups III, IV and V, in the form of Fe-Gly. The study covering the period from the first to the 42nd day of breeding revealed that the production performance and slaughter yield were not dependent on the form and amount of added Fe. In the experimental groups with the addition of Fe-Gly of 20 or 10 mg/kg, there were no deaths of chickens during the whole fattening period. As a result, introducing an organic form of iron covering 50% and 25% of the birds' requirement increased the effectiveness of chicken fattening (European Efficiency Index) (p < 0.01). An organic Fe compound (40, 20 or 10 mg/kg) added to mixtures contributed to significant changes in the level of biochemical and haematological indicators in blood. The study demonstrated that an addition of Fe-Gly to mixtures for broilers can be fully effective in terms of production and health performance even if the suggested requirement for this element is 50% or 25% covered.

  5. Effects of sub lethal concentration of Chloramin T on growth, survival, haematocrit and some blood biochemical parameters in common carp fry (Cyprinus carpio

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    Mohamad R. Imanpoor

    2011-07-01

    Full Text Available This study was done in Gorgan University of Agricultural Sciences and Natural Resources, in 2009, during 8 weeks to survey effects of different concentrations of Chloramin T on fry common carp (Cyprinus carpio (Linnaeus, 1758. According to the pre experiment data, lethal concentration, the lowest observed effect concentration, maximum allowable toxicant concentration and No Observed Effect Concentration of Chloramin T (Halamid in common carp (C. carpio fry were respectively 40.9, 27.1, 4.90 and 11.28 mg/L-1. Hence, the range of our experiment was between 0 to 25 mg L-1 which was divided to five treatments (0, 5, 10, 15 and 25 mg/L-1. At the end of experiment we calculated growth factor (special growth rate and food conversion ratio and stress indices (glucose total protein and percent of haematocrit and then they were compared with the controlled group. Our study results showed no significant difference between percentage of increase in body weight (280.4±25.79 - 200.4±10.16, special growth rate (2.34±0.25 - 1.96±0.06 and food conversion ratio (0.84±0.05 - 0.60±0.12 in experimental lots of fishes. There is also no significant difference between glucose (146.82±0.79 - 99.54±1.89 and total protein (3.61±0.45 - 2.82±0.06 in experimental groups (P>.05. However there is a significant difference between the percentages of haematocrit (57.34±4.99 - 40.74±2.17 and cholesterol (362.05±24.38 - 134.92±17.59 in these groups (P<0.05.

  6. Effect of the incorporation of date pits and orange pulp in rations composed of wheat straw and concentrate on the blood biochemical parameters of Ouled Djellal breeding

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    N. Lakhdara

    2014-12-01

    Full Text Available Twenty four lambs of Ouled Djellal breeding from the region of Constantine, Algeria, were assigned randomly into 4 groups, the mean initial weights within the groups, ranged between 37.6±4.27 and 39.8±5.41 kg, to investigate the effect of the incorporation of two by-products of food industry, fresh orange pulp, ground date pits in rations composed of wheat straw as roughage, and concentrate as supplement. Four feeding groups were formed, the first group (T1 was fed with wheat straw and concentrate (60%/40%, the second group (T2 with wheat straw and orange pulp (60%/40%, for group 3 and 4 (T3-T4, the diet consisted on a mixture of 60% wheat straw and date pits at a ratio of (80 to 20% as a roughage in addition to 40% orange pulp for T3 and 40% concentrate for T4. Blood samples were collected from the jugular vein before morning feeding. Values of animal's plasma levels of Ca, glucose, proteins and urea were measured using a UV spectrophotometer. There was no significant difference in all the diets for Ca value, Ca values varied between 8.37 and 10.74 mg/Dl. T4 showed the highest value. Glucose blood content was similar for all the animals with no significant differences. While a very significant difference <0.001 was observed in blood proteins level in T3 and T4 comparing to the other groups. When date pits were incorporated in the diet containing wheat straw and concentrate, a very significant difference on urea blood content of lambs was observed (P<0.001.

  7. The effects of different levels of Chlorella microalgae on blood biochemical parameters and trace mineral concentrations of laying hens reared under heat stress condition

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    Moradi kor, Nasroallah; Akbari, Mohsen; Olfati, Ali

    2016-05-01

    This study was conducted to investigate the effect of different supplementation levels of Chlorella microalgae on serum metabolites and the plasma content of minerals in laying hens reared under heat stress condition (27.5-36.7 °C, variable). A total number of 378 (40 weeks of age, with mean body weight of 1390 ± 120 g) were randomly allocated to six treatments with seven replicates. The birds were randomly assigned to 6 treatments (C, T1, T2, T3, T4, and T5) with 7 replicate cages of 9 birds. C. microalgae at the rates of 100, 200, 300, 400, and 500 ppm with water were offered to groups T1, T2, T3, T4, and T5, respectively, while group C served as a control. At 71 days of trial, blood samples (14 samples per treatment) were taken for measuring serum metabolites and at 72 days for plasma mineral analysis. The results of this experiment showed that the supplementation of 200-500 ppm C. microalgae decreased the serum content of cholesterol, triglycerides, and LDL ( P blood parameters of laying hens reared under heat stress.

  8. The effects of different levels of Chlorella microalgae on blood biochemical parameters and trace mineral concentrations of laying hens reared under heat stress condition

    Science.gov (United States)

    Moradi kor, Nasroallah; Akbari, Mohsen; Olfati, Ali

    2016-05-01

    This study was conducted to investigate the effect of different supplementation levels of Chlorella microalgae on serum metabolites and the plasma content of minerals in laying hens reared under heat stress condition (27.5-36.7 °C, variable). A total number of 378 (40 weeks of age, with mean body weight of 1390 ± 120 g) were randomly allocated to six treatments with seven replicates. The birds were randomly assigned to 6 treatments (C, T1, T2, T3, T4, and T5) with 7 replicate cages of 9 birds. C. microalgae at the rates of 100, 200, 300, 400, and 500 ppm with water were offered to groups T1, T2, T3, T4, and T5, respectively, while group C served as a control. At 71 days of trial, blood samples (14 samples per treatment) were taken for measuring serum metabolites and at 72 days for plasma mineral analysis. The results of this experiment showed that the supplementation of 200-500 ppm C. microalgae decreased the serum content of cholesterol, triglycerides, and LDL ( P < 0.05) whereas HDL content increased ( P < 0.05) in the hens supplemented with C. microalgae (300 or 400 and 500 ppm). C. microalgae at rates of 300-500 ppm caused a marked ( P < 0.05) increase in the plasma content of manganese or iodine and selenium but other minerals were not statistically different among treatments. Overall, from the results of the present experiment, it can be concluded that supplementation of C. microalgae at high rates was beneficial on blood parameters of laying hens reared under heat stress.

  9. Accelerated maximum likelihood parameter estimation for stochastic biochemical systems

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    Daigle Bernie J

    2012-05-01

    Full Text Available Abstract Background A prerequisite for the mechanistic simulation of a biochemical system is detailed knowledge of its kinetic parameters. Despite recent experimental advances, the estimation of unknown parameter values from observed data is still a bottleneck for obtaining accurate simulation results. Many methods exist for parameter estimation in deterministic biochemical systems; methods for discrete stochastic systems are less well developed. Given the probabilistic nature of stochastic biochemical models, a natural approach is to choose parameter values that maximize the probability of the observed data with respect to the unknown parameters, a.k.a. the maximum likelihood parameter estimates (MLEs. MLE computation for all but the simplest models requires the simulation of many system trajectories that are consistent with experimental data. For models with unknown parameters, this presents a computational challenge, as the generation of consistent trajectories can be an extremely rare occurrence. Results We have developed Monte Carlo Expectation-Maximization with Modified Cross-Entropy Method (MCEM2: an accelerated method for calculating MLEs that combines advances in rare event simulation with a computationally efficient version of the Monte Carlo expectation-maximization (MCEM algorithm. Our method requires no prior knowledge regarding parameter values, and it automatically provides a multivariate parameter uncertainty estimate. We applied the method to five stochastic systems of increasing complexity, progressing from an analytically tractable pure-birth model to a computationally demanding model of yeast-polarization. Our results demonstrate that MCEM2 substantially accelerates MLE computation on all tested models when compared to a stand-alone version of MCEM. Additionally, we show how our method identifies parameter values for certain classes of models more accurately than two recently proposed computationally efficient methods

  10. Assessment of biochemical concentrations of vegetation using remote sensing technology

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The main biochemicals (such as lignin, protein, cellulose, sugar, starch, chlorophyll and water) of vegetation are directly or indirectly involved in major ecological processes, such as the functions of terrestrial ecosystems (i.e., nutrient-cycling processes, primary production, and decomposition). Remote sensing techniques provide a very convenient way of data acquisition capable of covering a large area several times during one season, so it can play a unique and essential role provided that we can relate remote sensing measurements to the biochemical characteristics of the Earth surface in a reliable and operational way. The application of remote sensing techniques for the estimation of canopy biochemicals was reviewed. Three methods of estimating biochemical concentrations of vegetation were included in this paper: index, stepwise multiple linear regression, and stepwise multiple linear regression based on a model of the forest crown. In addition, the vitality and potential applying value are stressed.

  11. The in vitro effect of drugs on biochemical parameters determined by a SMAC system.

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    Vinet, B; Letellier, G

    1977-02-01

    1. We have studied the in vitro effect of 39 drugs on 17 biochemical parameters determined by a SMAC System. Only two drugs were found to interfere: ascorbic and theophyline. 2. The ascorbic acid lowers the glucose and the bilirubine values; it increases the creatinine and the uric acid concentration. At concentration smaller than 5 mg/dl of this drug, these effects are negligible. 3. We have found a new drug interference: theophylline inhibits the alkaline phosphatase and LDH activities. This effect is not negligible on alkaline phosphatase for therapeutic levels of this drug; the action on LDH can be ignored at normal therapeutic range. 4. For a given drug, we have found different interference with biochemical parameters determined with various commercial lyophlised control sera or a liquid pool of sera. This indicates that the type of sera used in drug interference studies must be described.

  12. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

    Institute of Scientific and Technical Information of China (English)

    Shumaila Kiran; Arif Mahmood Bhutta; Bakhtyawar Ali Khan; Sobia Durrani; Muhammad Ali; Muhammad Ali; Furhan Iqbal

    2012-01-01

    Objective: To report and compare the hematological and serum biochemical profile of goat and sheep from Rahim Yar Khan district in Southern Punjab. Methods: One hundred and twenty blood samples (98 goat and 22 sheep) were collected and their blood glucose, hemoglobin and serum biochemical parameters, cholesterol, aspartate transaminase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were determined by using spectrophotometer. Parameters were compared between goat and sheep. Results: It was observed that glucose (P=0.001), LDH (P=0.001) and ALT (P=0.001) concentrations differed significantly between sheep and goats. The studied parameters were compared between buck and goats and also between young goats (less than 12 months old) and adults (more than 12 months old). It was found that age and gender did not affect these hematological and serum biochemical parameters.Conclusions: It can be concluded that sheep have overall higher values than goats for studied hematological and serum biochemical parameters.

  13. Effect of toxoplasmosis and brucellosis on some biochemical parameters in ewes

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    N. A. J. Al- Hussary

    2010-01-01

    Full Text Available The present study was conducted to evaluate the effects of infection of ewes with toxoplasmosis and/or brucellosis on someserum biochemical parameters. Ninety six samples of blood were collected from aborted ewes at different stages of gestation,suspected to be infected with toxoplasmosis and /or brucellosis from different regions in Nineveh governorate. The percentageof toxoplasma and brucella infection depending on Latex Agglutination Test (LAT and Rose Bengal Test were 21.88% and23.96% respectively. The results of biochemical analysis showed that infection with toxoplasma caused significant elevation ofserum activities of aspartate aminotransferase (AST, alanine aminotransferase (ALT and lactate dehydrogenase (LDH. Aswell as the concentrations of glucose, total protein and copper. Where as the activity of alkaline phosphatase (ALP and theconcentration of calcium and zinc were reduced. Infection with brucellosis caused a significant increased in serum AST, ALTand LDH, ALP and creatin phosphokinase (CPK activities, and glucose, zinc and cholesterol concentrations. While serumcalcium and total protein concentration were decreased. Association of toxoplasma and brucella infection induced significantelevation of serum AST, ALT and LDH and CPK activities and the concentrations of glucose and copper with significant reduction in serum ALP activity and both calcium and zinc concentrations. It was concluded from this study that infection ofewes with toxoplasma and/or brucella caused changes in some biochemical parameters in the serum.

  14. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

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    Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

  15. Gadagi Magani Tea Is Detrimental Affects Biochemical Parameters

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    Umar A.A.

    2015-08-01

    Full Text Available Abstract Gadagi Magani tae is a herbal tea composed of many different herbs roots extracts shrubs and leaves prepared locally in form of tea consumed by many labourers youths tailors tricyclists and high way drivers in order to get extraordinary strength to endure hardship and overcome heavy duty within short period of time. The following biochemical parameters were routinely determined among consumers serum level of Aspartate aminotransaminases AST Alanine aminotransferase ALT activities were pragmatically higher than that of control increase in serum creatinine level lower sera urea and glucose levels and the sera levels of lipoproteins were also lower compared to controls. Hence Gadagi affects negatively the liver enzymes blood sugar level serum urea creatinine and carbonate positively as it lowers lipid profile.

  16. Effect of nitrate poisoning on some biochemical parameters in rats

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    M. B. Mahmood

    2011-01-01

    Full Text Available The present study was conducted to investigate the toxicity of potassium nitrate on glucose, cholesterol, alanine aminotransferase (ALT, aspartate aminotransferase (AST, and the possible ameliorative effect of ascorbic acid (Vitamin C. Male Wister rats are used as experimental model divided into three groups (each of 6-8 rats and treated for six weeks as follows: Group 1: served as control; Group 2: received 2 % potassium nitrate added to the forage and Group 3: received 2 % potassium nitrate together with 1 % ascorbic acid added to rat's forage. Nitrate treatment in group 2 leads to high significant increase levels of glucose in 3rd, 4th, and 5th weeks, cholesterol level increased significantly in both 4th and 5th weeks, while ALT levels increased in the 4th, 5th and 6th weeks, and AST increased significantly in the 5th and 6th weeks. Addition of ascorbic acid with potassium nitrate, lead to reverse all the parameters nearly to normal. It was concluded that potassium nitrate causes significant toxic effect on some biochemical parameters which was ameliorated by ascorbic acid.

  17. Evaluation of clinical, biochemical and hematological parameters in macrocytic anemia

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    Aarthi Kannan

    2016-07-01

    Results: Primary bone marrow disorders were the most common cause of macrocytosis (46%. The other causes in decreasing order of frequency were megaloblastic anaemia (38%, hemolytic anemia (6%, drug induced (5%, alcoholism and liver disease (4% and idiopathic thrombocytopenic purpura (1%. There was a significant difference in the mean values of MCV and serum LDH between megaloblastic and non and ndash; megaloblastic macrocytosis. When serum LDH >1345.2 IU/L or MCV>121fl (criterion values of ROC curve with reticulocyte count <2% was taken as criteria, the sensitivity was 92.1% and specificity was 93.5% for diagnosing megaloblastic anemia. Conclusions: Systematic evaluation of macrocytosis will help us to distinguish megaloblastic and non and ndash; megaloblastic macrocytosis. The blood and biochemical parameters especially CBC, RC, and serum LDH along with supporting clinical features help us in diagnosing megaloblastic anemia in a setup where vitamin and metabolite levels are difficult to obtain. [Int J Res Med Sci 2016; 4(7.000: 2670-2678

  18. Clinical and Biochemical Parameters of Children and Adolescents Applying Pesticides

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    O Hendy

    2010-06-01

    Full Text Available Background: The primary agricultural product in Egypt is the cotton crop. Children and adolescents work seasonally in the cotton fields applying pesticides.Objectives: To examine the effect of pesticide exposure on clinical and biochemical parameters in children and adolescents applying pesticides.Methods: Male children currently applying pesticides and aged between 9 and 19 years (n = 50 were recruited for this study. They were asked to complete work, health, and exposure questionnaires; examined for any medical and neurological problems with particular attention to sensory and motor functions including cranial nerves, sensory and motor system, and reflexes. From each participant, a blood sample was taken to measure acetylcholinesterase activity, and liver and kidney functions. Children who have never worked in agriculture (n = 50, matched on age, education, and socioeconomic status were also studied and served as controls.Results: More neuromuscular disorders were identified in pesticide applicators than controls. A significant lower level of acetylcholinesterase was found in the applicator group compared to the controls. There was also a significant difference in hematological, renal and hepatic indices in the exposed children compared to the control children. Working more days in the current season and also working more years as a pesticide applicator were both associated with an increase in the prevalence of neuromuscular abnormalities and significant changes in the laboratory tests.Conclusion: Children and adolescent pesticide applicators working in farms of Egypt are at risk of developing serious health problems similar to those of adults.

  19. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

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    Petra Hrabčáková

    2014-01-01

    Full Text Available The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P<0.05 in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P<0.05. At the end of the laying period, an increase (P<0.05 was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P<0.05 were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.

  20. Evaluation of nutritional and biochemical parameters in spontaneously hypertensive rats following antihypertensive treatment

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    Joanna Suliburska

    2014-03-01

    Full Text Available Introduction. One side effect of antihypertensive drugs is their impact on nutritional status and metabolism. The purpose of this study was to assess the nutritional and biochemical parameters in spontaneously hypertensive rats following treatment with antihypertensive drugs. Material and methods. The experiment was performed on 50 male spontaneously hypertensive rats (SHR, which were assigned to fi ve groups: control (C, with perindopril (PR, with metoprolol (MT, with indapamide (ID, and with amlodipine (AM. All rats were provided ad libitum standard diet (with or without drugs and distilled water. After 45 days, the animals were weighed and killed. Liver, kidney, heart, spleen, pancreas, and blood samples were collected. Concentrations of glucose, cholesterol, triglycerides, and albumin were assayed in serum. Morphology parameters, such as white blood cell, red blood cell, hematocrit, and lymphocyte counts were measured in the blood. Blood pressure was measured using a tail-cuff plethysmograph. Results. The results obtained indicate that the hypotensive drugs under investigation had no effect on the selected nutritional parameters. Perindopril signifi cantly decreased the relative mass of the heart and amlodipine markedly decreased the relative mass of the pancreas. A markedly higher concentration of glucose in the group with indapamid, and a signifi cantly lower concentration of triglycerides in the group with metoprolol, were observed. Indapamide and amlodipine markedly increased the value of red blood cells and hematocrit in the blood of SHR. Conclusions. Long-term therapy with antihypertension drugs may infl uence tissue mass and biochemical and morphological status in the body.

  1. [Design of high-efficiency double compound parabolic concentrator system in near infrared noninvasive biochemical analysis].

    Science.gov (United States)

    Gao, Jing; Lu, Qi-Peng; Peng, Zhong-Qi; Ding, Hai-Quan; Gao, Hong-Zhi

    2013-05-01

    High signal-to-noise ratio (SNR) of system is necessary to obtain accurate blood components in near infrared noninvasive biochemical analysis. In order to improve SNR of analytical system, high-efficiency double compound parabolic concentrator (DCPC) system was researched, which was aimed at increasing light utilization efficiency. Firstly, with the request of collection efficiency in near infrared noninvasive biochemical analysis, the characteristic of emergent rays through compound parabolic concentrator (CPC) was analyzed. Then the maximum focusing angle range of the first stage CPC was determined. Secondly, the light utilization efficiency of truncated type was compared with standard DCPC, thus the best structure parameters of DCPC system were optimized. Lastly, combined with optical parameters of skin tissue, calculations were operated when incident wavelength is 1 000 nm. The light utilization efficiency of DCPC system, CPC-focusing mirror system, and non-optical collecting system was calculated. The results show that the light utilization efficiency of the three optical systems is 1.46%, 0.84% and 0.26% respectively. So DCPC system enhances collecting ability for human diffuse reflection light, and helps improve SNR of noninvasive biochemical analysis system and overall analysis accuracy effectively.

  2. Bayesian inference of biochemical kinetic parameters using the linear noise approximation

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    Finkenstädt Bärbel

    2009-10-01

    Full Text Available Abstract Background Fluorescent and luminescent gene reporters allow us to dynamically quantify changes in molecular species concentration over time on the single cell level. The mathematical modeling of their interaction through multivariate dynamical models requires the deveopment of effective statistical methods to calibrate such models against available data. Given the prevalence of stochasticity and noise in biochemical systems inference for stochastic models is of special interest. In this paper we present a simple and computationally efficient algorithm for the estimation of biochemical kinetic parameters from gene reporter data. Results We use the linear noise approximation to model biochemical reactions through a stochastic dynamic model which essentially approximates a diffusion model by an ordinary differential equation model with an appropriately defined noise process. An explicit formula for the likelihood function can be derived allowing for computationally efficient parameter estimation. The proposed algorithm is embedded in a Bayesian framework and inference is performed using Markov chain Monte Carlo. Conclusion The major advantage of the method is that in contrast to the more established diffusion approximation based methods the computationally costly methods of data augmentation are not necessary. Our approach also allows for unobserved variables and measurement error. The application of the method to both simulated and experimental data shows that the proposed methodology provides a useful alternative to diffusion approximation based methods.

  3. Rhythmic 24-hour variations of frequently used clinical biochemical parameters in healthy young males - The Bispebjerg study of diurnal variations

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Gøtze, Jens Peter;

    2012-01-01

    Purpose. To evaluate the influence of time of day on the circulating concentrations of 14 frequently used clinical biochemical parameters in the Bispebjerg study of diurnal variations. Materials and methods. Venous blood samples were obtained under controlled environmental, activities and food...

  4. Effect of methanol extract ofSynsepalum dulciifcum pulp on some biochemical parameters in albino rats

    Institute of Scientific and Technical Information of China (English)

    Nkwocha Chinelo; Njoku Obioma Uzoma

    2015-01-01

    Objective:To determine the beneficial effects of the methanol extract ofSynsepalum dulcificum on some biochemical parameters. Methods: In this study, rats were orally administered (gavage) with methanol extracts at doses of 0 mg/kg (Group 1, as normal group), 100 mg/kg (Group 2), 200 mg/kg (Group 3) and 500 mg/kg (Group 4) body weight per day for 28 d. Results: Acute toxicity study showed that the methanol extract was not toxic to rats up to 5 000 mg/kg. From the results, the 100 mg/kg doses of the extract significantly (P<0.05) reduced serum levels of bilirubin, low density lipoprotein, alanine aminotransferase and glucose after 14 d compared with those after 28 d. A significant difference (P<0.05) was observed in the malondialdehyde and serum protein concentration in Group 4 while glucose concentration decreased significantly (P<0.05) in Group 1 and Group 4 after 14 d compared with 28 d. The high density lipoprotein significantly increased (P<0.05) in Group 3. Conclusions: The fruit has no negative effect on some biochemical parameters in albino rats.

  5. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

    Directory of Open Access Journals (Sweden)

    Prokić Marko D.

    2015-01-01

    Full Text Available Aspartame (ASP is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH, as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO and lipid peroxides (LPO in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  6. Biochemical studies on gamma irradiated male rats fed on whey protein concentrate

    International Nuclear Information System (INIS)

    This study carried out to investigate the possible role of whey protein protein concentrate in ameliorating some biochemical disorders induced in gamma irradiated male rats. Forty eight male albino rats were divided into four equal groups: Group 1 fed on normal diet during experimental period. Group 2 where the diet contain 15 % whey protein concentrate instead of soybean protein . Group 3 rats were exposed to whole body gamma radiation with single dose of 5 Gy and fed on the normal diet. Group 4 rate exposed to 5 Gy then fed on diet contain 15 % whey protein concentrate, the rats were decapitated after two and four weeks post irradiation. Exposure to whole body irradiation caused significant elevation of serum ALT, AST, glucose, urea, creatinine and total triiodothyronine with significant decrease in total protein, albumin and thyroxin. Irradiated rats fed on whey protein concentrate revealed significant improvement of some biochemical parameters. It could be conclude that whey protein concentrate may be considered as a useful protein source for reducing radiation injury via metabolic pathway.

  7. Blood biochemical parameters in male American mink (Neovison vison before and during the breeding season

    Directory of Open Access Journals (Sweden)

    Bogdan Lasota

    2014-05-01

    Full Text Available This study was aimed at selected blood biochemical parameters in the male American mink before (September-November and during the breeding (January-March season. Blood from 143 Black and Sapphire male mink at one year age was collected. The plasma was assayed for the concentration of glucose, total protein, cholesterol, HDL and LDL fractions, triacylglycerides (TG, and the activity of ALT and AST. Concentrations of glucose, protein, total and HDL/LDL cholesterol, and AST activity were generally slightly higher during the breeding season than during the non-breeding season, but remained within the reference range. In the case of ALT activity and TG concentration, the relations were reversed. The parameters studied in the Sapphire mink showed greater variation, both in- and out of the season. In Black and Sapphire males of the American mink, the studied parameters revealed slightly higher values during the breeding season than the non-breeding season. This will vary depending on the color variety. The decrease in TG concentration during the breeding season may indicate an increased energy demand due to ambient temperature falls, and/or may be a sign of increased energy consumption associated with physical exertion during mating. Consideration should be given to the nutrition of male mink during the breeding season.

  8. Seasonal variation of hemato-biochemical parameters in indigenous pig: Zovawk of Mizoram

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    P. Mayengbam

    2015-06-01

    Full Text Available Aim: To investigate the influence of season on erythrocyte picture, plasma enzyme and electrolyte profile of local pig of Mizoram at a different age. Materials and Methods: A volume of 2 ml of blood samples were collected from 72 Zovawk pigs of three different age groups viz. pre-weaning, grower and adult pigs reared in College of Veterinary Sciences and Animal Husbandry Selesih, Aizawl, Mizoram, India, livestock farm during summer and winter in order to find out influence of season on erythrocyte picture, enzyme and electrolyte profile. The hematological parameters were estimated by using an automatic blood analyzer. The biochemical parameters were estimated by using diagnostic kits. Results: The grower pigs had lower hemoglobin (Hb, packed cell volume (PCV, mean corpuscular volume (MCV, mean corpuscular Hb (MCH during summer, rainy and winter seasons (p<0.05 and lower MCH concentration (MCHC during summer season (p<0.05. Adult pigs had lower Hb with higher total erythrocyte count in rainy season. PCV and MCV of adult pigs decreased in summer (p<0.05. Serum glutamic oxaloacetic transaminase activity of both the grower and adult groups increased during summer and rainy seasons (p<0.05. Zovawk pigs had higher sodium (Na and lower potassium (K in rainy season (p<0.05. Pre-weaning piglets had higher Na, K, calcium (Ca, and magnesium (Mg in summer than in winter. Grower pigs had higher Na, K and Ca in summer (p<0.05. Pi of pre-weaning and grower groups was higher in winter. Adult pigs had lower alkaline phosphatase activity and Ca in summer and higher Pi and Mg in winter (p<0.05. Conclusion: Seasonal variation in some hemato-biochemical parameters of Zovawk pig were investigated. Influence of season on the hemato-biochemical profile was most evident during the grower stage, followed by adults and pre-weaning piglets.

  9. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

    OpenAIRE

    Mirjana Joksimović Todorović; Vesna Davidović

    2012-01-01

    The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA), activity of enzymes of aspartat aminotransferase (AST), alanin aminotransferase (ALT) and gamma glutamyltranferase (GGT), in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartu...

  10. Haematological and biochemical blood parameters in horses used for sport and recreation

    Directory of Open Access Journals (Sweden)

    Katarzyna BURLIKOWSKA

    2015-12-01

    Full Text Available The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P<0.05 red blood cell count, haematocrit value and haemoglobin concentration as well as the serum level of total protein, albumin, α2- and γ-globulin, HDL and iron. The level of β- globulin, magnesium and activity of creatine kinase were significantly higher in recreationally used horses. There were no differences in the values of red blood cell parameters (MCV, MCH, MCHC, number of leucocytes and the percentage of their respective kinds, platelets, concentration of urea, creatinine, glucose, triglycerides, total cholesterol, LDL, activity of AST, ALT, LDH and in the content of Na, K, Cl, P, Ca between the tested groups. The results of the present study indicate that the type of training influences some of the resting haematological and biochemical indices in adult horses. Horses used for show jumping have higher aerobic capacity and show a more beneficial lipid profile compared to horses used for recreation.

  11. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    Directory of Open Access Journals (Sweden)

    R.N. Sani

    2016-04-01

    Full Text Available This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring, August and October (401 samples in summer. California Mastitis Test (CMT, milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial culture and CMT as were deemed to have glands with subclinical mastitis (SCM. The prevalence of subclinical mastitis in our study was around 12% (in one year. Of 144 (18.2% and 47 (11.72% positive samples in CMT, 134 (16.94% and 44 (10.97% samples were positive for bacterial culture in spring and summer, respectively. Comparison of results of hematological and serum biochemical parameters in ewes with SCM and healthy ewes in spring and summer showed that WBC counts, total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (P˂0.05. The results of the present study indicated that when interpreting hematological and serum biochemical parameters in ewes with SCM should be consider the effect of season on these parameters.

  12. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    Directory of Open Access Journals (Sweden)

    R.N. Sani

    2016-06-01

    Full Text Available This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring, August and October (401 samples in summer. California Mastitis Test (CMT, milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial culture and CMT as were deemed to have glands with subclinical mastitis (SCM. The prevalence of subclinical mastitis in our study was around 12% (in one year. Of 144 (18.2% and 47 (11.72% positive samples in CMT, 134 (16.94% and 44 (10.97% samples were positive for bacterial culture in spring and summer, respectively. Comparison of results of hematological and serum biochemical parameters in ewes with SCM and healthy ewes in spring and summer showed that WBC counts, total protein concentrations and plasma fibrinogen in both groups of ewes were significantly higher in spring than summer (P˂0.05. The results of the present study indicated that when interpreting hematological and serum biochemical parameters in ewes with SCM should be consider the effect of season on these parameters.

  13. Long-term changes in microbial and biochemical parameters in the Central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Raghukumar, C.; Nath, B.N.; Sharma, R.; LokaBharathi, P.A.; Dalal, S.G.

    Natural kilometer-scale spatial variability and seasonal variability in deepsea sediments at ~5000 m depth was studied at 2 reference sites in the Central Indian Basin. Biochemical parameters such as the labile organic matter (consisting...

  14. Effects of angiotensin converting enzyme inhibitor: ramipril on different biochemical parameters in essential hypertensive patients

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    Pratibha S. Salve

    2016-06-01

    Conclusions: Ramipril has beneficial effects on RAS (Renin angiotensin system and kinin system or both may contribute to the improvement in different biochemical parameters by ramipril. [Int J Res Med Sci 2016; 4(6.000: 2288-2291

  15. Relationship between daily dietary fructose intake, body composition and biochemical parameters patients with type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Sabriye Arslan

    2016-05-01

    Conclusion: Further studies are needed to develop more specific suggestions regarding fructose intake and to better understand the relationship between fructose intakes, body composition and biochemical parameters in individuals with type 2 diabetes.

  16. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

    OpenAIRE

    Petra Hrabčáková; Eva Voslářová; Iveta Bedáňová; Vladimíra Pištěková; Jan Chloupek; Vladimír Večerek

    2014-01-01

    The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P ...

  17. Biochemical Response of Glycine Max after Inoculation of Different Concentrations of Fusarium Solani

    Directory of Open Access Journals (Sweden)

    N. Hamid

    2014-06-01

    Full Text Available Diseases of agricultural crops are old as agriculture itself. It has been estimated that fungal diseases are responsible for an annual reduction for more than 20% of total potential world food production from crop plants. Randomized complete block design experiments were conducted to study the effect of different colonies of Fusarium solani on some biochemical and physiological parameters of Glycine max. Sterilized seeds of Glycine max (L. Merr was artificially inoculated with conidia of 3 different concentrations (10,000, 100,000 and 1000, 000 cfu. Seedlings of Glycine max was grown in 350g acid washed sand. Complete Nutrient Hoagland solution was regularly used to irrigate the plants. Leaves samples were weekly collected for analysis of biochemical tests. It was observed that inoculation of Fusarium solani significantly increase the concentration of total soluble carbohydrate and reducing sugar in inoculated plants over healthy tissues. Protein analysis revealed a significant reduction with the development of disease. Activity of invertase illustrate a variable change initially it was increased than afterward declined.

  18. Variations of clinical biochemical parameters of laying hens and broiler chickens fed aflatoxin-containing feed.

    Science.gov (United States)

    Fernandez, A; Verde, M T; Gascon, M; Ramos, J; Gomez, J; Luco, D F; Chavez, G

    1994-03-01

    Two groups of 32 laying hens (Hyssex Brown) and two groups of 32 23-day-old (Hybro) broiler chickens were fed 2.5 and 5 parts/10(6) of aflatoxin in their diet for 4, 8, 16 and 32 days; 16 hens and 32 chicks were maintained as control groups (0 parts/10(6)). After the intoxication period, a clearance period was established of 1, 2, 4 and 8 days. Relative weights of liver and kidneys significantly increased in intoxicated hens, but not in broiler chickens. Histological lesions in both types of bird consisted of hepatic cell vacuolation with fatty infiltration. There was a significant decrease (Phens, cholesterol levels were not significantly (P> 0.05) different from control values, but triglyceride levels decreased (PAST) serum levels remained normal, whereas alanino aminotransferase (ALT) activity decreased in both intoxicated groups. The activity of serum lactic dehydrogenase (LDH) and gammaglutamil transferase (GGT) increased significantly. In intoxicated broiler chickens, aflatoxins did not alter (P> 0.05) the biochemical parameters studied, except that the serum calcium concentration was lower in the 5 parts/10(6) group. These data indicated that in intoxicated laying hens, a severe clinical biochemical alteration was produced, and that this together with the hepatic lesions observed in hens and broilers may aid disease diagnosis.

  19. Variation in biochemical parameters in different parts of potato tubers for processing purposes.

    Science.gov (United States)

    Bandana; Sharma, Vineet; Kaushik, S K; Singh, Brajesh; Raigond, Pinky

    2016-04-01

    The present study was conducted to estimate the variation in bio-chemical parameters among eight different parts viz. bud end cortex, bud end medulla, central cortex, central medulla, pith, stem end cortex, stem end medulla and peel of potato tuber of processing varieties. Concentration of dry matter, reducing sugar, sucrose and starch content were higher in cortical region than in medullar region of stem end, bud end and central portion. Variety Kufri Chipsona-1 had maximum dry matter content in stem end cortex (SEC 30.34 %), followed by Kufri Frysona (SEC 27.71 %). Mean reducing sugar values were comparatively more in bud end cortex (BEC 111.3 mg/100 g Fresh Weight) and lowest in stem end medulla (SEM 44.05 mg/100 g FW). Bio-chemical contents varied considerably within different parts of tuber as well as in different genotypes. The information generated in this study can help processors for effective utilization of potato for various types of processing products viz., chips and French fries. PMID:27413232

  20. Effects of ramadan fasting on cardiovascular and biochemical parameters

    Directory of Open Access Journals (Sweden)

    M Gupta

    2013-12-01

    Full Text Available Background: Muslims abstain from food and drink from dawn to sunset every day during Ramadan - the holiest month in Islamic lunar calendar.  Methods: The effect of Ramadan fast on body mass index (BMI, blood pressure (BP, fasting blood glucose (FBG and lipid profile were studied on 100 healthy male, adult Muslim volunteers. All parameters were recorded one week before the onset and then in the last week of Ramadan month and compared.  Results: There was no statistically significant effect on BMI, and systolic or diastolic blood pressures (p>0.05. However, fasting blood glucose (FBG, serum total cholesterol (TC, serum triglycerides (TG were significantly decreased (p = 0.000 and high density lipoprotein cholesterol (HDL-C level was significantly (p=0.000 increased in last week of Ramadan fasting compared to pre fasting levels. Conclusion: Our results show beneficial effects of Ramadan fasting on FBG and serum lipid profile. 

  1. Effects of methanolic extract of Moringa oleifera leaves on semen and biochemical parameters in cryptorchid rats.

    Science.gov (United States)

    Afolabi, Ayobami Oladele; Aderoju, Hameed Adeola; Alagbonsi, Isiaka Abdullateef

    2013-01-01

    While anti-oxidant effects of Moringa oleifera in much oxidative stress related diseases have been well reported, cryptorchidism on the other hand has been shown to cause oxidative stress. However, study is scanty on the likely role of Moringa oleifera in reducing cryptorchidism-induced oxidative stress in rats has not been studied. The present study looked into the effects of methanolic extract of Moringa oleifera leaves (MEMO) on semen and biochemical parameters in cryptorchid rats. Twenty male albino rats (200-250 g) were randomly divided into 4 groups (n=5 each). Groups A and B were sham-operated and treated with corn-oil and 200 mg/kg of MEMO respectively, while groups C and D were rendered cryptorchid and also treated with corn-oil and 200 mg/kg of MEMO respectively. Cryptorchid rats had lower testicular weight, sperm count, germ cell count, testicular superoxide dismutase (SOD) concentration, testicular total protein and higher testicular malondialdehyde (MDA) concentration compared to sham-operated rats. MEMO had no significant effect on testicular weight and MDA concentration, while it significantly increased sperm count, germ cell count, testicular SOD and total protein in the cryptorchid rats. The present study suggests that MEMO ameliorates cryptorchidism associated germ cell loss and oxidative stress.

  2. THE EFFECTS OF VEGETABLE LECITHIN ON SOME SANGUINE BIOCHEMICAL PARAMETERS AT LACTATING SHEEP AND GOATS

    Directory of Open Access Journals (Sweden)

    D. DRINCEANU

    2013-12-01

    Full Text Available From a complex experiment of establishing the effects of lecithin supplemented in the diet of small ruminants, in this paper we present the influence of lecithin on some sanguine biochemical parameters at lactating sheep and goat. The researches were made on a experimental group formed by 5 sheep from łurcana breed and 5 goats from Crapatina breed, which were fed in the first experimental period with a base ratio (Br formed by 1.5 kg of hill hay and 0.2 kg concentrate forage mixture, in the second experimental period the Br was supplemented with 100 g lecithin/day/animal. At the end of each experimental period were taken blood samples to establish some biochemical parameters from sanguine serum. The supplementation of base ration with 100 g lecithin/day/animal at lactating sheep and goats did not produced significant changes to values of total proteins (TS, albumin, creatinine, urea and total bilirubins (TB from sanguine serum. Contrary to expectation the cholesterol and triglycerides were higher than normal both in sheep (from 82 mg/dl to 83 mg/dl for cholesterol and from 23 mg/dl to 45 mg/dl for triglycerides but specially in goats (from 59 mg/dl to 68 mg/dl for cholesterol and from 24 mg/dl to 55 mg/dl for triglycerides. Were registered significant increment of activity of alkaline phosphatase and in γ – GT and a decrease of plasmatic activity of pancreatic α-amylase both in sheep and goats.

  3. Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna.

    Science.gov (United States)

    Bownik, Adam; Stępniewska, Zofia; Skowroński, Tadeusz

    2015-02-01

    Ectoine (ECT) is a compatible solute produced by soil, marine and freshwater bacteria in response to stressful factors. The purpose of our study was to determine the possible toxic influence of ECT on Daphnia magna. We determined the following endpoints: survival rate during exposure and recovery, swimming performance, heart rate, thoracic limb movement determined by image analysis, haemoglobin level by ELISA assay, catalase and nitric oxide species (NOx) by spectrophotometric methods. The results showed 80% survival of daphnids exposed to 50mg/L of ECT after 24h and 10% after 90h, however lower concentrations of ECT were well tolerated. A concentration-dependent reduction of swimming velocity was noted at 24 and 48h of the exposure. ECT (at 2.5 and 4mg/L) induced an increase of heart rate and thoracic limb movement (at 2.5, 4 and 20mg/L) after 24h. After 10h of the exposure to ECT daphnids showed a concentration-dependent increase of haemoglobin level synthesized and accumulated in the epipodite epithelia. After 24h we noted a concentration-dependent decrease of haemoglobin level and its lowest value was found after 48h of the exposure. ECT at a concentration of 20 and 25mg/L slightly stimulated catalase activity after 24h. NOx level was also increased after 10h of the exposure to 20 and 25mg/L of ECT reaching maximal activity after 24h. Our results suggest that ECT possesses some modulatory potential on the behaviour, physiology and biochemical parameters in daphnids. PMID:25460046

  4. Effect of Different Protein Levels On Nutrient Digestion Metabolism and Serum Biochemical Parameters in Calves

    Institute of Scientific and Technical Information of China (English)

    LI Hui; DIAO Qi-yu; ZHANG Nai-feng; TU Yan; WANG Ji-feng

    2008-01-01

    The current study has been performed to examine the effects of different dietary protein levels on the growth,nutrient digestion and absorption,as well as biochemical parameters in calves.Nine healthy newborn calves were selected,randomly divided into 3 groups and fed 3 milk replacers with different protein levels(18,22,and 26%),respectively.Five period-digestion-metabolism trials were taken between 12-20,22-30,32-40,42-50,and 52-60 days after birth.All 3 groups showed a similar growth curve during 11 to 61 experimental days,however,the growth rate of the 22%crude protein(CP) group was 8.89%higher than that of the 18%CP group and 19.48%higher than that of the 26%CP group.respectively. The apparent digestibility of dry material(DM)declined gradually with age,whereas,the apparent digestibility of N,extract ether(EE)rose slightly.Compared to the 22%CP and 26%CP group,calves fed with 18%CP apparently had a lower digestibility than DM,EE,and nitrogen(N).The average apparent digestibilities of N were 69.39,75.36,and 74.55%, respectively.Both the apparent digestibility and retention of calcium(Ca)and phosphorus(P)were steady throughout the experiment,but the average apparent digestibility of P in the 26%CP group was only 63.83%,which was markedly lower than that of the 18%CP group(70.40%)and 22%CP group(69.73%).In addition,the sernm concentrations of total protein(TP),albumin(ALB),and globulin(GLOB)of the 22%CP group were higher than those in the 18%CP and 26%CP groups.The urea N(BUN)content in the 18%CP group,on the other hand,was significantly lower than that of the other two groups.The highest glucose(GLU)concentration was found in the 22%CP group(5.38 mmol L-1),at the end of the trials.The protein levels in the milk replacer affected the digestion metabolism of nutrition and the serum biochemical parameters of calves at different physiological phases.Calves fed with 22%CP milk replacer had a better growth performance than the other groups.

  5. Relationship Between Hyperspectral Parameters and Physiological and Biochemical Indexes of Flue-Cured Tobacco Leaves

    Institute of Scientific and Technical Information of China (English)

    LI Xiang-yang; LIU Guo-shun; YANG Yong-feng; ZHAO Chun-hua; YU Qi-wei; SONG Shi-xu

    2007-01-01

    The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.

  6. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

    Directory of Open Access Journals (Sweden)

    Mirjana Joksimović Todorović

    2012-06-01

    Full Text Available The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA, activity of enzymes of aspartat aminotransferase (AST, alanin aminotransferase (ALT and gamma glutamyltranferase (GGT, in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartum. The highest values of total leukocytes count, neutrophiles, monocytes and basophiles count were recorded on the day of parturition, and then decreased, so that the minimum value established on d 45 of lactation. The lymphocytes count was the lowest on the day of parturation, while the eosinophils and basophiles counts were significantly different in observed time intervals. The concentration of glucose ranged from 3.07 mmol/L on d 15 prepartum to 2.71 mmol/L on d 15 postpartum. A high concentration of non-esterified fatty acids (NEFA in the postpartum period is a consequence of changes in energy balance and more intensive process of lypolisis in fat tissue, due to energy disbalance at the beginning of lactation. Established activity of enzymes AST, ALT and GGT was approximately the same in studied time intervals and was within physiological limits. The results of hematological and biochemical analysis in this paper do not indicate the development of postpartum ailments, because the investigated parameters were within physiological limits.

  7. Developmental dynamics of some blood biochemical parameters in the growing turkey (Meleagris gallopavo).

    Science.gov (United States)

    Szabó, A; Mézes, M; Horn, P; Süto, Z; Bázár, Gy; Romvári, R

    2005-01-01

    Blood serum clinical biochemical parameters of fasted BUT Big 8 male turkeys were determined at the ages of 3 days, 4, 8, 12, 16 and 20 weeks, for a follow-up of the developmental changes of some serum metabolites, enzymes and ions. The serum protein content (total protein, albumin, globulin) increased with age, indicating also the moulting-associated metabolic changes in the age interval from the 8th to the 12th weeks. Creatinine was shown to have a peak at 3 days of age (role of muscle activity in thermogenesis), while urate concentration sensitively reflected the dietary protein amount. Serum triglycerides peaked at the time of yolk catabolism, while cholesterol was shown to indicate the moulting, as was serum malondialdehyde. Serum sodium content increased throughout the study. Alanine aminotransferase and aspartate aminotransferase activities increased along the ontogeny, while alkaline phosphatase activity decreased in parallel with the growth. Serum creatine kinase activity showed an over one-magnitude increase. General metabolic and enzymatic alterations were characteristic and applicable for the description of the ontogenetic development of a precocial (post-hatch triglyceride peak), large bodied, meat-type (lactate dehydrogenase, continuously increasing creatine kinase) bird species.

  8. Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids

    Directory of Open Access Journals (Sweden)

    Mladenović Jelena M.

    2014-01-01

    Full Text Available The effects of subchronic exposure to copper (Cu on lipid peroxidation, hemato-biochemical parameters, and the possible protective role of flavonoids Quercetin and (--Epicatechin were studied. Male Wistar albino rats were treated with Cu (560 mg/L, p.o. as CuCl2•2H2O for 5 weeks and Quercetin and (--Epicatechin (40 mg/kg BW each, i.p., every third day during the last 3 weeks alone or in combination. Cu increased the concentration of lipid peroxides, decreased the number of erythrocytes, hemoglobin and hematocrit values and increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase. Coadministration of Quercetin and (--Epicatechin with Cu lowered the process of lipid peroxidation and restored examined hemato-biochemical parameters to control values. Our results indicate that Cu induced oxidative damage in erythrocytes, which led to anemia, while Quercetin and (--Epicatechin showed a protective effect on the hemato-biochemical processes in the blood of rats. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  9. Stability of Intercellular Exchange of Biochemical Substances Affected by Variability of Environmental Parameters

    Science.gov (United States)

    Mihailović, Dragutin T.; Budinčević, Mirko; Balaž, Igor; Mihailović, Anja

    Communication between cells is realized by exchange of biochemical substances. Due to internal organization of living systems and variability of external parameters, the exchange is heavily influenced by perturbations of various parameters at almost all stages of the process. Since communication is one of essential processes for functioning of living systems it is of interest to investigate conditions for its stability. Using previously developed simplified model of bacterial communication in a form of coupled difference logistic equations we investigate stability of exchange of signaling molecules under variability of internal and external parameters.

  10. Parameters Obtained by Hepatobiliary Scintigraphy Have Significant Correlation with Biochemical Factors Early After Liver Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Kansoul, H.A.; Axelsson, R.; Yamamoto, S.; Savicheva, I.; Aspelin, P.; Ericzon, B.G.; Gjertsen, H. [Div. of Transplantation Surgery and Div. of Radiology, Dept. of Clinical Science, Intervention, and Technology, Karolinska Inst., Karolinska Univ. Hospital, Stockholm (Sweden)

    2007-08-15

    Background: Early postoperative hepatobiliary scintigraphy after liver transplantation is performed worldwide, but data on its significance for graft function are currently limited. Purpose: To examine the correlation between the result of early postoperative hepatobiliary scintigraphy and pre- and postoperative biochemical parameters in liver transplantation (LTx) patients. Material and Methods: Six parameters of hepatobiliary scintigraphy using {sup 99m}Tc mebrofenin were statistically analyzed in 108 LTx patients: 1) half-life of the activity of elimination of mebrofenin from the blood; 2) total clearance of mebrofenin from the blood due to all possible routes; 3) half-life of the activity due to liver uptake; 4) clearance of mebrofenin from the blood due to liver uptake; 5) time to maximal uptake in the liver; and 6) the hepatic extraction fraction (HEF) and biochemical data. Analysis between patients with preoperative normal liver function, familial amyloid polyneuropathy (FAP), and end-stage liver disease (non-FAP) was also performed. Results: Univariate and multivariate analysis revealed that total bilirubin postoperative day 3 correlated with all three scintigraphic parameters, and peak aspartate aminotransferase and alanine aminotransferase correlated with HEF. The analysis between patients with FAP and non-FAP revealed no significant difference of scintigraphic data between the two groups. Conclusion: A significant correlation between early postoperative scintigraphic results and biochemical parameters was demonstrated.

  11. Basal blood parameters of horses subjected to aerobic activity fed with lipidic concentrated

    Directory of Open Access Journals (Sweden)

    Kátia de Oliveira

    2012-02-01

    Full Text Available The feeding diets were evaluated containing low and high levels of soybean oil for horses athletes subjected to two protocols of aerobic training on the response of basal blood biochemical parameters. Four horses were used in latin square design with treatments in a 2 x 2 factorial arrangement. Treatments consisted levels of 5 and 15% oil concentrates and two aerobic training, 40' and 60' minutes. Plasmatic parameters were monitored, triglyceride (TG, total cholesterol (TC, glucose (GLU and lactate (LAC, during basal metabolism. The TG, TC, GLU and LAC from horses at rest were not affected (P> 0.05 neither of diet and physical activity, 0.21, 3.79, 4.18, 0.93 mmol L-1, respectively. It can be concluded that offer concentrate with high content of soybean oil to athletic horses in aerobic activities can be performed without altering the blood biochemical profile of basal metabolism.

  12. Effect of toxoplasmosis and brucellosis on some biochemical parameters in ewes

    OpenAIRE

    N. A. J. Al- Hussary; A. S. M. Al- Zuhairy

    2010-01-01

    The present study was conducted to evaluate the effects of infection of ewes with toxoplasmosis and/or brucellosis on someserum biochemical parameters. Ninety six samples of blood were collected from aborted ewes at different stages of gestation,suspected to be infected with toxoplasmosis and /or brucellosis from different regions in Nineveh governorate. The percentageof toxoplasma and brucella infection depending on Latex Agglutination Test (LAT) and Rose Bengal Test were 21.88% and23.96% re...

  13. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

    OpenAIRE

    Fabrícia de Souza Predes; Sérgio Luis Pinto da Matta; Juliana Castro Monteiro; Tânia Toledo de Oliveira

    2009-01-01

    This study was carried out to evaluate the effects of Arctium lappa L. (burdock) on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT), total protein, total cholesterol, urea, uric acid, triacylglycerol,...

  14. The effects of adenosine on plasma homocysteine levels and some other biochemical parameters

    OpenAIRE

    Turgut, Günfer; Rota, Simin; Aybek, Hülya; Turgut, Sebahat; Sert, Seelahattin; Genç, Osman

    2009-01-01

    Süleyman Demirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2007 Aralık; 14(4) The effects of adenosine on plasma homocysteine levels and some other biochemical parameters Günfer Turgut*, Simin Rota**, Hülya Aybek**, Sebahat Turgut*, Selahattin Sert**, Osman Genç* *Pamukkale University Faculty of Medicine Departments of Physiology, Denizli, Turkey **Pamukkale University,Faculty of Medicine, Departments of Biochemistry Denizl...

  15. Effect of ferrous sulphate on haematological, biochemical and immunological parameters in neonatal calves

    OpenAIRE

    Lobna S. Elgebaly; Adel M.A. Eisa

    2010-01-01

    The effect of oral administration of iron on haematological, biochemical and immunological parameters in neonatal calves was studied. Ten calves from a private farm in Gharbia Governorate were used. Calves were separated from their dams immediately after birth and received colostrum during the first hours after calving and twice daily for 48 h. Thereafter, they received whole milk. Calves were divided into two equal groups. The first group was kept as controls. Calves of the second group were...

  16. Impact of sudarshan kriya yoga on mean arterial blood pressure and biochemical parameters in medical students

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    Nisar Ahmad

    2016-06-01

    Conclusions: Living a happy and healthy life on all planes is possible through the unified practice of sudarshan kriya yoga (SKY along with asana and pranayam. The practice of SKY seems to be beneficial on mean blood pressure and biochemical parameters in normal healthy individuals. This unique breathing practice is a potent energizer. [Int J Res Med Sci 2016; 4(6.000: 2150-2152

  17. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

    OpenAIRE

    Prokić Marko D.; Paunović Milica G.; Matić Miloš M.; Đorđević Nataša Z.; Ognjanović Branka I.; Štajn Andraš Š.; Saičić Zorica S.

    2015-01-01

    Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased i...

  18. ESTIMATING BIOCHEMICAL PARAMETERS OF TEA (CAMELLIA SINENSIS (L.)) USING HYPERSPECTRAL TECHNIQUES

    OpenAIRE

    Bian, M.; Skidmore, A.K.; Schlerf, M.; Liu, Y; Wang, T.

    2012-01-01

    Tea (Camellia Sinensis (L.)) is an important economic crop and the market price of tea depends largely on its quality. This research aims to explore the potential of hyperspectral remote sensing on predicting the concentration of biochemical components, namely total tea polyphenols, as indicators of tea quality at canopy scale. Experiments were carried out for tea plants growing in the field and greenhouse. Partial least squares regression (PLSR), which has proven to be the one of th...

  19. Effects of D2O on biochemical parameters of normal cells and tumour cells

    International Nuclear Information System (INIS)

    The influence of high temperatures (Hyperthermia) on normal tissue and Ehrlich-Ascites tumour cells ('ATZ') was examined under several conditions with regard to the application of deuterium oxide as a stabilising factor. It was proven that the DNA-synthesis of normal tissue (liver, mouse) is not sensitive to temperature. This effect of hyperthermia only occurs when the tissue is damaged, e.g. by trypsinising. The influence of hyperthermia on several biochemical parameters and on morphological changes of the Ascites cells was examined. The findings show that deuterium oxide (D2O) is able to reduce both the thermal and the ureal denaturation of enzymes. Thus tests were carried out to find out if D2O also reduces toxic influence in complicated biological systems. The assumption of high D2O concentrations to prevent several reactions was confirmed. When the Ascites tumour cells in the H2O-buffer were exposed to the damaging influence of hyperthermia, the high degree of damage was seen with the decreasing DNA synthesis, reduced aerobic glycose capacity, a drop in the ATP values and breakdown of the permeability of the membrane. Deuterium oxide was able under high temperature (from appr. 440C on) to reduce the degree of damage to DNA synthesis, while auto-effects (inhibition of synthesis) of D2O predominate in the lower region. Aerobic glycolysis was damaged in both cases to the same degree, however. In D2O after hyperthermia the ATP-level dropped faster than in H2O. D2O not only reduces the thermal denaturation of the Ascites tumour cells, but it also eliminates the toxic influence of the zytostaticum TRENIMONsup(R) (under 380 or 460C incubation). (orig./AJ)

  20. Effects of Race Distance on Physical, Hematological and Biochemical Parameters of Endurance Horses

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    Lawan Adamu

    2010-01-01

    Full Text Available Problem statement: Endurance horses are generally exposed to severe stress during endurance competitions. These horses are however conditioned to cover the long distances of the competitions at moderate speeds. Approach: This study was conducted in Malaysia to determine the post-race hematological, blood electrolyte and biochemical and physical parameters of horses of 40, 80 and 120 km endurance race categories and eliminated and horses that completed the races with good performance. Whole blood, plasma and serum samples were collected after each race. Results: After physical examination 7 (9.72% 120 km-, 48 (66.67% 80 km- and 17 (23.61% 40 km-race horses were eliminated. Eight horses, all from the 80 km-race category completed the race with good performance. The mean heart of the good performance horses (74.2±13.9 beats min-1 was higher than that of the eliminated 40 km- (68.2±14.7 beats min-1, 80 km-race (62.9±9.7 beats min-1 horses. The blood lactate concentration of good performance horses was 9.2±2.2 mmol L-1, which was significantly higher than in the 40 km- (6.0±2.9 mmol L-1, 80 km- (6.7±3.2 mmol L-1 and the 120 km-race (6.4±1.6 mmol L-1 horses. The blood glucose concentration was lower in the good performance horses (1.6±0.9 mmol L-1 than the eliminated 40 km- (5.7±1.9 mmol L-1, 80 km- (4.3±2.4 mmol L-1 and the 120 km-race (5.0±1.4 mmol L-1 horses. Conclusion: The study showed that eliminated horses exhibited poorer glucose utilization than good performance horses, which may have resulted in poor lactate production. Thus the blood lactate and glucose concentrations of horses during training may be used to predict their performance in endurance races.

  1. The effects of salt stress on growth and biochemical parameters in two maize varieties

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    Elżbieta Sacała

    2014-02-01

    Full Text Available The main objective of this study was to examine the influence of salinity on growth and biochemical parameters (NR activity, amino compound accumulation, protein content, some inorganic ion concentrations of two varieties of maize (Limko and Koka. Salinity (75.0 mol • m-3 NaCl and 37.5 mol • m-3 Na2SO4 significantly reduced fresh and dry weights of the investigated maize varieties. NaCl induced higher reduction in biomass production than Na2SO4. Differences in salt tolerance between Limko and Koka were small.It was found that in both maize varieties the nitrate reductase activity decreased under salt stress (100 mol • m-3 NaCl. This effect was more marked in the Koka variety than Limko. Decrease in nitrate reductase activity had no inhibitory effect on accumulation of protein and free amino compounds. Salinity treatment (100 mol • m-3 NaCl led to an increase in free amino compound contents in roots and shoots of both investigated maize varieties, but these changes were not very large. The highest increase in amino compound level was observed in roots of Koka and it was 2-fold higher than in control plants. Salt stress did not change soluble protein contents in Limko and Koka varieties with the exception of Limko leaves. There was significant increase in soluble protein content on leaves of Limko and it amounted 128% of the control. Salinity caused a great increase in leaf and root Na+ concentration and a decrease in case of Ca2+ and K+ contents. The declines in Ca2+ content in maize roots were 76 and 70% respectively for Koka and Limko roots. The fall in K+ concentration was high, but not so drastic as in Ca2+. In roots of Koka and Limko the reduction in K+ content was approximately 30%. Higher reduction in K+content showed shoots of Limko. In this case K+ content lowered to 49% of control.

  2. Predictive value of routine hematological and biochemical parameters on 30-day fatality in acute stroke

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    Bhatia R

    2004-04-01

    Full Text Available OBJECTIVE: This prospective study was planned to study the prognostic value of routine clinical, hematological and biochemical parameters, including platelet aggregation in patients of acute stroke, on fatality occurring during the first 30 days. MATERIAL AND METHODS: In this study 116 consecutive patients (77 males and 39 females of stroke (within 72 hours of onset were included. After clinical evaluation and neuroimaging, blood investigations, hemoglobin, total leukocyte count, platelet count, platelet aggregation, erythrocyte sedimentation rate (ESR, blood sugar, urea, creatinine, sodium, potassium, serum cholesterol, serum bilirubin, aspartate aminotransferase (SGOT, alanine aminotransferase (SGPT, albumin, and globulin estimations were performed. The patients were followed up for a maximum period of 30 days from the onset of stroke, and patients who expired were grouped as ′expired′ and the rest as ′survivors′. Logistic regression analysis was carried out among the significant parameters to identify independent predictors of 30-day fatality. RESULTS: Univariate analysis demonstrated that among hematological parameters, high total leukocyte count and ESR, at admission, correlated significantly with an undesirable outcome during the initial 30 days. Among biochemical parameters, elevated urea, creatinine, serum transaminases (SGOT and SGPT and globulin levels correlated significantly with death. Logistic regression analysis demonstrated that a low Glasgow Coma Scale (GCS score along with biochemical parameters such as high serum creatinine, SGPT, ESR and total leukocyte count correlated with death. CONCLUSION: Impaired consciousness, high total leukocyte count, raised ESR, elevated creatinine and SGPT levels, estimated within 24 hours of hospitalization, are the most important indicators of 30-day mortality in patients with first-time ischemic stroke.

  3. Determination of Parameters of PV Concentrating System With Heliostat

    Science.gov (United States)

    Vardanyan, R.; Norsoyan, A.; Dallakyan, V.

    2010-10-01

    The structure of PV concentrating system with heliostat is analyzed. The mathematical model of system consisting of PV concentrating module and heliostat is developed. With the use of developed mathematical model the optimal parameters of the system are determined. The results of this work can be used during the design of PV concentrating systems with heliostats.

  4. Pharmacodynamic interaction of fenugreek, insulin and glimepiride on sero-biochemical parameters in diabetic Sprague-Dawley rats

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    C. Haritha

    2015-05-01

    Full Text Available Aim: This study was undertaken to assess the pharmacodynamic interaction of fenugreek, insulin and glimepiride on serobiochemical parameters in streptozotocin-induced diabetic rats. Materials and Methods: A total of 56 male Sprague-Dawley rats, randomly divided into seven Groups. Group 1: Non-diabetic control; Group 2: Streptozotocin induced diabetic control; Groups 3, 4 and 5 were treated with insulin, glimepiride and fenugreek seed powder, respectively; Groups 6 and 7: Insulin + fenugreek seed powder treatment and glimepiride + fenugreek seed powder treatment respectively, in diabetic rats. Body weights, blood glucose, lipids total cholesterol (TC, high-density lipoprotein (HDL, low-density lipoprotein (LDL, triglycerides (TG and proteins (total protein, albumin, globulin, A/G ratios were studied at different time intervals. Rats were sacrified at the end of 8 weeks, pancreas and aorta collected for histopathological study. Results: The results of Group 2 showed significantly (p<0.05 higher concentration of glucose, TC, TG, LDL, globulin, A/G ratios and significantly (p<0.05 lower concentration of albumin, total protein, HDL and body weights when compared to Group 1 at the end of 4th and 8th weeks intervals with marked alteration in histopathology of pancreas and aorta. All the treatment Groups 3-7 showed significantly (p<0.05 improvement in the all the parameters and the Groups 6 and 7 showed highest decrease in the concentration blood glucose, TC, TG, LDL and increase in the albumin, total protein and body weights during 6th and 8th week, respectively. Conclusion: The treatment with fenugreek, insulin and glimepiride countered the alteration in the sero biochemical parameters in diabetic rats, and their combination was found a positive interaction in improving the sero biochemical status of diabetic rats.

  5. Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro

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    Sharuti Rathore

    2014-05-01

    Full Text Available Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in productivity. It is essential to test important medicinal plants for their salinity tolerance as research efforts aim to explore economic benefits under saline conditions. Keeping in view the importance of Stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two Stevia genotypes. Two node microcuttings were subjected to MS media supplemented with different NaCl concentrations (0, 25, 50, 75, 100, 125 mM. Chlorophyll amount was observed to be decreased as compared to sugars, proline and phenols with increased salt concentrations.

  6. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation

    International Nuclear Information System (INIS)

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  7. Effects of a low-radiotoxicity uranium salt (uranyl acetate) on biochemical and hematological parameters of the catfish, Clarias gariepinus

    Science.gov (United States)

    Al-Ghanim, Khalid A.; Ahmad, Zubair; Al-Kahem Al-Balawi, Hmoud F.; Al-Misned, Fahad; Maboob, Shahid; Suliman, El-Amin M.

    2016-01-01

    Specimens of Clarias gariepinus were treated with lethal (70, 75, 80, 85, 90, and 95 mg/L) and sub-lethal concentrations (8, 12 and 16 mg/L) of uranyl acetate, a low-radiotoxicity uranium salt. The LC 50 value was registered as 81.45 mg/L. The protein and glycogen concentrations in liver and muscles were decreased in the fish exposed to sub-lethal concentrations. The red blood cell (RBC) and white blood cell (WBC) counts, haemoglobin (Hb) concentration and haematocrit (Hct) values were decreased. Different blood indices like mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were negatively affected. Level of plasma glucose was elevated whereas protein was decreased. The level of calcium concentration (Ca) was declined in the blood of exposed fish whereas magnesium (Mg) remains unchanged. The activity level of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) was elevated in exposed fish. These effects were more pronounced in the last period of exposure and in higher concentrations. Results of the present study indicate that uranyl acetate has adverse effects on Clarias gariepinus and causes changes in the biochemical and hematological parameters of the fish.

  8. Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)

    Science.gov (United States)

    Feng, G.; Zhuang, P.; Zhang, L.; Kynard, B.; Shi, X.; Duan, M.; Liu, J.; Huang, X.

    2011-01-01

    The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160mgL-1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140mgL-1 group was significantly higher than the level in 80, 100 and 120mgL-1 groups. Aspartate aminotransferase (AST) activity in the 140mgL-1 group was significantly higher than those in the 100 and 120mgL-1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180mgL-1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180mgL-1 were significantly higher than those of 60 and 90mgL-1. Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120mgL-1 and 60-90mgL-1, respectively. Clove oil was a promising alternative to MS-222. ?? 2011 Blackwell Verlag, Berlin.

  9. The Hypertrophic Marchigiana: physical and biochemical parameters for meat quality evaluation

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    F. M. Sarti

    2010-04-01

    Full Text Available The aim of this study was to evaluate the meat quality of double muscled Marchigiana young bulls characterized by different genotypes for the hypertrophy: normal and mutated (heterozygous. Calpain and calpastatin activities were determined to verify the state of aging meat on a sample of Longissimus thoracis muscle (XIII thoracic rib taken at slaughtering (0h and after 24 hours (24h. After 14 days of aging, another sample of muscle was taken to evaluate physical and chemical parameters of meat quality. The results showed a better meat quality of mutated animals respect normal animals. Another interesting result was the correlation between the biochemical parameters and some physical parameters, such as WBS (Warner Bratzler Shear Force, CL (Cooking loss. These results showed the relationship between the proteolytic activity of calpain system and meat tenderness.

  10. THE EFFECT OF LUTEIN ADDITIVES ON BIOCHEMICAL PARAMETERS IN BLOOD OF LAYING HENS

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    Manuela Grčević

    2016-06-01

    Full Text Available The aim of this study was to present the performance and some biochemical parameters in the blood of hens fed feed mixtures with different levels of lutein. The study involved a total of 291 Tetra SL hybrid laying hens, divided into 3 groups (O0 with 0 mg/kg lutein added, O200 with 200 mg/kg lutein added and O400 with 400 mg/kg lutein added, and lasted for 5 weeks. The O0 group included 93 hens, whereas experimental groups 98 and 100 hens, respectively. The highest egg production and the best feed consumption per egg were recorded in O400 group with a total of 3367 pieces of eggs produced and 128.60 g of feed consumed. The best laying intensity (96.68% and number of eggs per laying hen (34.19 as well as the highest feed consumption per day (129.01 g, was recorded in O200 group. Lutein added to feed mixtures for laying hens did not significantly affect the values of biochemical parameters in blood (P>0.05. An increase in total cholesterol (CHOL and HDL cholesterol content as well as the decrease of total protein (PROT and albumin (ALB content in the experimental groups can be observed. The content of glucose (GUK and urea was similar between the experimental groups, while the highest content of triglycerides (TGC was recorded in O200 group. It can be concluded that different levels of lutein added to the feed mixtures for laying hens did not have negative effect on the performance and blood biochemical parameters of laying hens.

  11. Prediction of outcome utilizing both physiological and biochemical parameters in severe head injury.

    Science.gov (United States)

    Low, David; Kuralmani, Vellaisamy; Ng, See Kiong; Lee, Kah Keow; Ng, Ivan; Ang, Beng Ti

    2009-08-01

    Traumatic brain injury is a major socioeconomic burden, and the use of statistical models to predict outcomes after head injury can help to allocate limited health resources. Earlier prediction models analyzing admission data have been used to achieve prediction accuracies of up to 80%. Our aim was to design statistical models utilizing a combination of both physiological and biochemical variables obtained from multimodal monitoring in the neurocritical care setting as a complement to earlier models. We used decision tree and logistic regression analysis on variables including intracranial pressure (ICP), mean arterial pressure (MAP), cerebral perfusion pressure (CPP), and pressure reactivity index (PRx), as well as multimodal monitoring parameters to assess brain tissue oxygenation (PbtO(2)), and microdialysis parameters to predict outcomes based on a dichotomized Glasgow Outcome Score. Further analysis was carried out on various subgroup combinations of physiological and biochemical parameters. The reliability of the head injury models was assessed using a 10-fold cross-validation technique. In addition, the confusion matrix was also used to assess the sensitivity, specificity, and the F-ratio. In all, 2,413 time series records were extracted from 26 patients treated at our neurocritical care unit over a 1-year period. Decision tree analysis was found to be superior to logistic regression analysis in predictive accuracy of outcome. The combined use of microdialysis variables and PbtO(2), in addition to ICP, MAP, and CPP was found have the best predictive accuracy. The use of physiological and biochemical variables based on a decision tree analysis model has shown to provide an improvement in predictive accuracy compared with other previous models. The potential application is for outcome prediction in the multivariate setting of advanced multimodality monitoring, and validates the use of multimodal monitoring in the neurocritical care setting to have a potential

  12. [Simulation of vegetation indices optimizing under retrieval of vegetation biochemical parameters based on PROSPECT + SAIL model].

    Science.gov (United States)

    Wu, Ling; Liu, Xiang-Nan; Zhou, Bo-Tian; Liu, Chuan-Hao; Li, Lu-Feng

    2012-12-01

    This study analyzed the sensitivities of three vegetation biochemical parameters [chlorophyll content (Cab), leaf water content (Cw), and leaf area index (LAI)] to the changes of canopy reflectance, with the effects of each parameter on the wavelength regions of canopy reflectance considered, and selected three vegetation indices as the optimization comparison targets of cost function. Then, the Cab, Cw, and LAI were estimated, based on the particle swarm optimization algorithm and PROSPECT + SAIL model. The results showed that retrieval efficiency with vegetation indices as the optimization comparison targets of cost function was better than that with all spectral reflectance. The correlation coefficients (R2) between the measured and estimated values of Cab, Cw, and LAI were 90.8%, 95.7%, and 99.7%, and the root mean square errors of Cab, Cw, and LAI were 4.73 microg x cm(-2), 0.001 g x cm(-2), and 0.08, respectively. It was suggested that to adopt vegetation indices as the optimization comparison targets of cost function could effectively improve the efficiency and precision of the retrieval of biochemical parameters based on PROSPECT + SAIL model.

  13. Changes in biochemical, strength, flexibility, and aerobic capacity parameters after a 1700km ultraendurance cycling race

    OpenAIRE

    Vicente Javier Clemente-Suarez

    2014-01-01

    The purpose of the present research was to study the organic response after ultraendurance cycling race. Selected biochemical, leg strength, flexibility, and aerobic capacity parameters were analyzed in 6 subjects 5 days before and 5 days after completing a 1700 km ultraendurance cycling race. After the race, participants presented a significant decrease in Hb (167.8 ± 9.5 versus 141.6 ± 15.7 mg/dL), strength (29.4 ± 2.7 versus 25.5 ± 3.7 cm in a countermovement jump), and oxygen upt...

  14. Investigation of hematological and biochemical parameters in obese women in reproductive age

    OpenAIRE

    Kara IH et al.

    2012-01-01

    Objective: The purpose of this article is to analyze the relationship between the body mass indices and hematological and biochemical parameters of reproductive women at the age of 18 to 49 years old.Method: This cross-sectional and descriptive study includes 124 consecutive reproductive women patients at the age between 18 and 49 and the mean age is 33.6±7.3, admitted to Düzce University Faculty of Medicine, Department of Family Medicine Obesity and Check-up Outpatient Clinics between June 2...

  15. Effect of Cadmium and Zinc on growth and Biochemical Parameters of Selected Vegetables.

    Directory of Open Access Journals (Sweden)

    Davika Bhargava

    2013-05-01

    Full Text Available Heavy metals occur naturally in soil but more is being released into the environment by humans especially from mining. Heavy metals are helpful for plants in mineral nutrition but in excess they cause pollution and are harmful for plants, invertebrates and some vertebrate fishes. Through precipitation of their compounds or by ion exchange into soil heavy metal pollutants can localize and lay dormant. They have inhibitory effects on plant growth and the performance of photosynthetic apparatus of plants. The present paper reports the adverse effects of zinc and cadmium on growth and biochemical parameters of Lady’s finger and Cluster bean.

  16. Effect of ascorbic acid on morphological and biochemical parameters in tomato seedling exposure to salt stress

    Directory of Open Access Journals (Sweden)

    Krupa-Małkiewicz Marcelina

    2015-06-01

    Full Text Available The aim of this study is to determine the effect of both NaCl and KCl alone and in comparison to AsA on the morphological and some biochemical parameters of Oxheart and Vilma cultivars of tomato under laboratory and field conditions. A combination of salt applied in the laboratory experiment caused a significant effect on seed germination and root and shoot length and a significant reduction of Chl a, Chl b and Car contents in 14-day-old tomato seedlings. However, seedlings of cultivar Vilma were characterised by higher tolerance to applied salt stress.

  17. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    OpenAIRE

    R.N. Sani; M. Moezifar

    2016-01-01

    This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring), August and October (401 samples in summer). California Mastitis Test (CMT), milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial cultur...

  18. Effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Ana Angélica Henrique Fernandes

    2009-02-01

    Full Text Available Amongst the numerous co-adjuvant therapies which could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of naringerin on biochemical parameters in streptozotocin-induced (STZ - 60 mg/kg, i.p. diabetic rats. Male rats were divided into four groups: G1: untreated controls; G2: normal rats receiving naringerin; G3: untreated diabetics; G4: diabetics rats receiving naringerin. The naringerin (50mg/kg, i.p, decreased the hyperglycaemia and hyperlipidaemia associated with STZ-diabetes. The concentrations of serum insulin in treated diabetic rats tended to be increased. Naringerin treatment prevents STZ-induced changes in the activities of ALT, AST and LDH in the liver and heart, indicating the protective effect of naringerin against the hepatic and cardiac toxicity caused by STZ. The glycogen level in cardiac and hepatic tissues elevated with naringerin in diabetic rats. The naringerin can improve the glucose and lipid metabolism and is beneficial in preventing diabetic complications.Dentre as numerosas terapias para minimizar as complicações diabéticas, os antioxidantes e flavonoides são testados na clínica médica. Foi analisado o efeito da naringerina sobre os parâmetros bioquímicos em ratos diabéticos induzidos por estreptozotocina (STZ - 60mg/kg, i.p.. Ratos machos foram divididos em 4 grupos: G1: controle não tratado; G2: ratos normais que receberam naringerina; G3: diabéticos não tratados; G4: ratos diabéticos que receberam naringerina. Naringerina (50mg/kg, i.p., decresceu a hiperglicemia e a hiperlipidemia em ratos diabéticos. A concentração sérica de insulina em ratos tratados tendeu aumentar. A naringerina preveniu as alterações, provocadas pela estreptozotocina, na atividade hepática e cardíaca de ALT, AST e LDH, indicando o efeito protetor da naringerina sobre estes tecidos, contra toxicidade

  19. Influence of Fasciola Hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

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    A Hodžić

    2013-03-01

    Full Text Available Background: The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver.Methods: Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n=34 and infected group (n=29. For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used.Results: Biochemical analysis of tested parameters showed a significant elevation (P≤0.05 of serum gamma-glutamyl transferase (GGT, total bilirubin (TBIL and direct bilirubin (DBIL in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST between groups. Vascular and biliary systems of the liver were found to be affected.Conclusion: Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica.

  20. Observed Changes in Some Biochemical Parameters After Magnetic Field Exposure to 1,5 Tesla

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    Turan Akdağ

    2012-10-01

    Full Text Available Aim: In daily life, individuals are exposed to electromagnetic contamination at different levels. Magnetic resonance imaging (MRI is a radiodiagnostic imaging technique using non-ionizing radiofrequency radiation. Considering possible adverse effects of magnetic fields may occur at long time, complications are difficult to determined earlier. Therefore, the effects of MRI on some biochemical parameters were evaluated before and after MRI.Material and Method: In the present study, applying for MRI and with no systemic disorders, total 40 volunteers between the ages of 25-45 were enrolled. Blood samples were investigated before and after MRI, and subjects were exposed to magnetic field of 1.5 T for 30 min. Blood samples were soon centrifuged and studied in terms of the levels of serum Ca, Fe, Fe binding, triglyceride, cholesterol, TT3, TT4, free T3, free T4, TSH, ferritin, insulin, lipase, HDL, LDL and glucose. Results: In the study, levels of Ca, cholesterol, triglyceride, HDL, TT3, Fe, Fe binding, lipase, TSH and insulin after MRI were found to be lower than those before the procedure. This changs was observed to be statistically significant(P<0.05, and the difference related to levels of LDL, glukoz, ferritin, Free T3, Free T and TT4 were found to be statistically insignificant. Discussion: It was concluded that some biochemical parameters ocur after MRI, and further studies are needed to evaluate longterm consequences and biological effects and whether the changes are temporary.

  1. Risk of prostate cancer and its correlation with different biochemical parameters in non diabetic men

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    Neha Sharma

    2013-08-01

    Full Text Available Background: It has been hypothesized that men with long term diabetes have a lower risk of prostate cancer then non-diabetic men. Whether diabetes influences level of biomarkers such as prostate specific antigen (PSA, which is involved in the detection of prostate cancer is, unknown. In view of the aforementioned controversial literature, it was decided to evaluate this relation-ship in non-diabetic men. We evaluated the correlation between fasting glucose, prostate specific antigen and different biochemical lipid profile parameters with serum uric acid and serum creatinine in non-diabetic male between age group 40-61 years. Methods: Association between fasting serum glucose , different lipid parameters, serum uric acid, serum creatinine and prostate specific antigen in 83 non-diabetic males aged 40 to 61years were studied retrospectively. Glucose and lipid parameters and serum creatinine, serum uric acid were measured on fully automated analyser using standard reagent kits. Serum prostate specific antigen was measured by TOSOH-AIA-360, immunoassay method. Results: Correlations between different biochemical parameters were determined. Prostate specific antigen were negatively correlated with HDL (r= -0.22, p= 0.03 in age group 40-61 years. At the same fasting blood sugar were correlated positively(r= 0.34, p= 0.02 with prostate specific antigen in age group 51-60 years , but not in age group 40-50 years. Conclusion: We concluded that serum HDL (high density lipoprotein was negatively associated and FBS (fasting blood sugar was positively associated with risk of prostate cancer. We also suggest that in men of this age group a low HDL level should not be ignored while assessing prostate cancer risk especially if accompanied with an elevated FBS level even in the upper normal range. [Int J Res Med Sci 2013; 1(4.000: 476-481

  2. Potential of liquid extracts of Sargassum wightii on growth, biochemical and yield parameters of cluster bean plant

    Institute of Scientific and Technical Information of China (English)

    N Vijayanand; S Sivasangari Ramya; S Rathinavel

    2014-01-01

    Objective:To explore biofertilizing efficiency of seaweed liquid extracts of brown alga Sargassum wightii (S. wightii) on growth, biochemical and yield parameters of Cyamopsis tetragonoloba (C. tetragonoloba). Methods: Seaweeds were made to coarse powder and stock solution was prepared. Different concentrations such as 0.5%, 1.0%, 1.5%, 2.0%, 2.5%and 5.0%were prepared and given as foliar spray. Results: Seaweed Liquid Extract (SLE) at low concentration (1.5%) exhibited promoting effect on growth and yield parameters. Differential responses in the content of photosynthetic pigments, protein, reducing sugar, ascorbic acid and in the activity of nitrate reductase were also observed in the leaves of SLE treated seedlings when compared to untreated seedlings. Higher concentrations (above 1.5%) of SLE were found to show inhibitory effect. Conclusion:The presence of micro and macro nutrients, vitamins, growth hormones and other constituents in the seaweed extract might be very much useful to the crops but their level should be appropriate to enhance growth and productivity. It may be concluded that liquid seaweed extracts could serve as cost effective eco-friendly product for sustainable agriculture.

  3. Changes in biochemical, hemodynamic, and dialysis adherence parameters in hemodialysis patients during Ramadan.

    Science.gov (United States)

    Alshamsi, Shaikha; Binsaleh, Fatima; Hejaili, Fayez; Karkar, Ayman; Moussa, Dujana; Raza, Hamad; Parbat, Parkash; Al Suwida, Abdulkareem; Alobaili, Saad; AlSehli, R; Al Sayyari, Abdulla

    2016-04-01

    This paper aimed to study the effect of Ramadan fasting on biochemical and clinical parameters and compliance for dialysis. A prospective multicenter observational cross-sectional study comparing fasting with a non-fasting stable adult hemodialysis patients for demographic and biochemical parameters, compliance with dialysis, inter-dialytic weight gain, pre- and post-blood pressure, and frequency of intradialytic hypotensive episodes was carried out. Six hundred thirty-five patients, of whom 64.1% fasted, were studied. The fasters were younger (53.3 ± 16.2 vs. 58.4 ± 16.1 years; P = 0.001) but had similar duration on dialysis (P = 0.35). More fasters worked (22.0% vs. 14.6%; P = 0.001) and missed dialysis sessions during Ramadan. No differences were noted between groups in sex, diabetic status, or dialysis shift or day. There were no differences in the pre- and post-dialysis blood pressure; serum potassium, albumin or weight gain; diabetic status; sex; and dialysis shift time or days. However, serum phosphorous was significantly higher in the fasting group (2.78 ± 1.8 vs. 2.45 ± 1.6 mmol/L; P = 0.045). There were no intragroup differences in any of the parameters studied when comparing the findings during Ramadan with those in the month before Ramadan. Fasters were significantly younger and more likely to be working, to miss dialysis sessions, and to have higher serum phosphorous levels. No other differences were observed. PMID:26420510

  4. Hybrid Differential Evolution for Estimation of Kinetic Parameters for Biochemical Systems

    Institute of Scientific and Technical Information of China (English)

    ZHAO Chao; XU Qiaoling; LIN Siming; LI Xuelai

    2013-01-01

    Determination of the optimal model parameters for biochemical systems is a time consuming iterative process.In this study,a novel hybrid differential evolution(DE)algorithm based on the differential evolution technique and a local search strategy is developed for solving kinetic parameter estimation problems.By combining the merits of DE with Gauss-Newton method,the proposed hybrid approach employs a DE algorithm for identifying promising regions of the solution space followed by use of Gauss-Newton method to determine the optimum in the identified regions.Some well-known benchmark estimation problems are utilized to test the efficiency and the robustness of the proposed algorithm compared to other methods in literature.The comparison indicates that the present hybrid algorithm outperforms other estimation techniques in terms of the global searching ability and the convergence speed.Additionally,the estimation of kinetic model parameters for a feed batch fermentor is carried out to test the applicability of the proposed algorithm.The result suggests that the method can be used to estimate suitable values of model parameters for a complex mathematical model.

  5. Microbial Succession And Biochemical Aspects Of Mandai Fermentation At Low Salt Concentration.

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    Hasrul Satria Nur

    2009-04-01

    Full Text Available Mandai isfermented food that traditionally made from the flesh of jack fruit (Arthocarphus champeden Spreg.. Usually mandai ismade as high salt concentration. However, the objective of this research is to study the succession and biochemicalaspects of microbials during fermentation at low salt concentration (10% w/v for 14 days. During the period offermentation microbial cell numbers and biochemical aspects were observed at the 3rd, 5th, 7th and 14th day. Total cellnumber of bacteria and yeast were measured by pour plate method. The measurement was also conducted on fleshbefore incubation. The biochemical aspect observation included the contents of reducing sugar, N-total, salt, and pH.The research results indicated that the pattern of microbial succession occurred the fermentation. Yeast cells grewdominantly (2.8 x 109 CFU/g on the 5th day fermentation but bacteria were dominant at the end (1.1 x 107 CFU/g. Thehighest decrease of reducing sugar and N-total contents were 0.240% at the 14th day and 0.159% at the 5th day,respectively. However, salt concentration was relatively stable and pH was varied within the range of 3.71-6.12 for thewhole period fermentation.

  6. Acute Effects of Hexavalent Chromium on Survival, Oxygen Consumption, Hematological Parameters and Some Biochemical Profiles of the Indian Major Carp, Labeo rohita

    OpenAIRE

    Vutukuru, S. S.

    2005-01-01

    An attempt has been made in the present investigation to determine the acute toxicity of hexavalent chromium and its toxicological effects on survival, physiological, hematological and biochemical parameters of the widely consumed Indian major carp, Labeo rohita. Short-term acute toxicity tests were performed adopting renewal bioassay technique (USEPA, 1975) over a period of 96h, using different concentrations of potassium dichromate to the fish and the 96 h LC50 value was found to be 111.45 ...

  7. The dynamics of biochemical parameters in blood of clinically healthy holstein cows from day 5 before to day 60 after calving

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    Irena Celeska

    2015-10-01

    Full Text Available The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and BHBA. Milk production and body condition score were also estimated. Obtained results showed that cows were exposed to mild to marked metabolic distress. Energy status was changed due to increased values of NEFA and BHBA and decreased value of glucose after calving. Protein concentrations were increased at day 10 after calving, despite the decrease of the level of albumin. Urea concentrations before and after calving were within physiological range indicating an optimal protein diet. Increased values of total bilirubin at day 5 after calving indicated liver increased activity. Lipid status presented by triglycerides and total cholesterol revealed no differences in blood concentrations. Milk production was highest at day 30 after calving. BCS were highest in dry cows, thereafter they declined and recovered at day 60 after calving. In conclusion, biochemical parameters can be used as relevant indicators of metabolic distress in cows around calving with milk and BCS recording as aside parameters. Changes in some biochemical parameters indicate liver increased activity and metabolic stress, that could lead to decreased milk production, impaired reproductive performance and, finally, to illness.

  8. Changes in biochemical parameters on the first day after kidney transplantation: risk factors for nosocomial infection?

    Institute of Scientific and Technical Information of China (English)

    YANG Yi; REN Liang; ZHANG Yong; LIU Hang; CAO Bin; ZHANG Xiao-dong

    2010-01-01

    Background Nosocomial infection in early post-transplantation period is a tough problem for kidney transplantation. Few reports have explored the relations between biochemical parameters and nosocomial infection in kidney transplantation. This retrospective study was carried out to describe the characteristics of nosocomial infection in the very early period of kidney transplantation and to determine the risk factors in biochemical parameters and their alterations. Methods Patients who underwent their first kidney transplantation from January 2001 to March 2009 in Beijing Chao-Yang Hospital were recruited and the nosocomial infectious episodes were collected for this study. Gender, age, donor type, delayed graft function (DGF) and biochemical parameters such as serum uric acid, lipids files and albumin on day 0 (before transplantation) and day 1 (24 hours after transplantation) and their changes were analyzed with Logistic regression models for nosocomial infection. Results A total of 405 patients (315 men and 90 women) were involved in this study. There were 80 patients experiencing 113 infection episodes and 105 strains of microorganism were indentified. In univariate analysis, there were significant differences in DGF, albumin on day 0, lipoprotein (a) (Lp(a)) on day 1, change in low density lipoprotein-cholesterol (LDL-C, day 1-day 0) and change in uric acid (day 1-day 0) between nosocomial infection patients and noninfectious patients (P<0.05). In multivariate analysis, change in uric acid (day 1-day 0) (Off 5.139, 95% Cl 1.176-22.465, P<0.05), change in LDL-C (day 1-day 0) {OR4.179, 95% Cl 1.375-12.703, P<0.05) and DGF (Of? 14.409, 95% Cl 1.603-129.522, P<0.05) were identified as independent risk factors for nosocomial infection in kidney transplantation. Conclusions Most nosocomial infections in early postoperative period of kidney transplantation are bacterial, especially with Gram-negative bacteria. The most common infection sites are respiratory tract

  9. Effect of Root Kudzu on Biochemical Parameters in Streptozotocin-Induced Diabetic Rats

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    Abas Rashidi

    2015-04-01

    Full Text Available Introduction: Diabetes, characterized by high blood glucose levels, is a serious chronic disease and antioxidant play an important role in the pathophysiology, complications, and management of the disease. Kudzu root, a traditional Chinese herbal medicine, has a long history of use for the treatment of headaches, cough, edema and extracts are a rich source of isoflavones and antioxidants property. The aim of this study was to evaluate the effect of the kudzu root on biochemical parameters in streptozotocin-induced diabetic rats. Methods: Male wistar rats were divided into four groups (n=32 including: Control, diabetic, diabetic rats treated with 100 mg/kg kudzu root extract, and diabetic rats treated with 50mg/kg kudzu root extract. Diabetes was induced by STZ intraperitoneally administration (50 mg/kg and kudzu root extract was gavaged at the dose of 50 and 100 mg/kg for five weeks. Blood biochemical factors and insulin serum levels were also assessed. Results: The results show that in STZ induced diabetic rats, the serum levels of FBS, total cholesterol (TC and triglyceride (TG increased whiles the levels of HDL and Insulin were decreased. Moreover in diabetic kudzu treatment groups, the serum levels of of FBS, total cholesterol (TC and triglyceride(TG decreased (P≤0.001 whiles the serum level of HDL and Insulin were increased. Conclusion: In addition the antioxidant effects, Kudzu root extract produced a significant anti-hyperglycemic effect on STZ-induced diabetic rats.

  10. NOVEL INVESTIGATIONS ON THUJA OCCIDENTALIS EXTRACT ON RABBIT HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

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    Mansoor Ahmad

    2013-03-01

    Full Text Available Thuja occidentalis (family: Cupressaceae has been used as antiseptic, expectorant, diuretic and in the treatment of cancer and removal of kidney stones in different systems of medicine. The present studies were carried out on T. occidentalis leaves extract to evaluate the hematological and biochemical effects on rabbits. The animals were divided in to control (A and B and treated (C and D male and female groups (n=5. There was a slight decrease in Hemoglobin, Red blood cell count and Hematocrit/Packed cell volume values, while a significant increase in platelet count was observed in male and female treated groups. However, slight increase in total leukocyte count value in male treated group and decrease count with female treated group was also observed. Urea, Creatine phospho kinase, Creatine phospho kinase iso-enzyme, Direct Bilirubin, Gamma-glutamyl transpeptidase, Cholesterol and Albumin/Globulin ratio values were elevated in male treated group. While Phosphorus, Uric acid, Lactate dehydrogenase, Serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, Alkaline Phosphatase, Cholesterol, Triglycerides, High density lipoprotein, Low density lipoprotein, Very-low-density lipoprotein, Globulin and Blood Glucose values were found low. In female treated group almost same results were observed like male treated group except that hypoglycemic effect was more pronounced in female treated group. These findings suggested that when results of hematological and biochemical parameters of test groups (C & D were compared with their respective controls (A & B, significant changes were observed which contributes towards the diversified therapeutic effect of T. occidentalis.

  11. Comparison of biochemical parameters of rainbow trout (Oncorhynchus mykiss) reared in two different trout farms'

    Science.gov (United States)

    Karatas, Tayfun

    2016-04-01

    The aim of this study was to compare biochemical parameters of cultured rainbow trouts (Oncorhynchus mykiss, Walbaum, 1972) reared in two different trout farms' (Agri and Erzurum). The average weights of fish were 150±10gr for first station (Agri), 230±10gr for second station (Erzurum). Fishes used in research were randomly caught from pools, and fifteen pieces were used for each group. Fishes were fed with commercial trout feed with 45-50% crude protein twice a day. The levels of AST, ALT, LDL, total cholesterol and triglyceride in the second station (Erzurum) were found to be higher (pLDL, triglyceride may be associated with size, sex, sexual maturity and environmental conditions (temperature, pH, hardness and dissolved oxygen).

  12. Effect of induced epilepsy on some biochemical parameters in female rats

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    J.S. H. Ali

    2012-01-01

    Full Text Available The activity of cholinesterase and some biochemical parameters of blood such as glucose, cholesterol and phospholipids were estimated in 52 epilepsy induced females of Wister albino rats. Animals of this experiment were divided into two groups, group (I regarded as control and group (II administrated subcutaneously by pentylenetetrazole 100mg/kg and divided in to three sub-groups according to the time of samples collection 3 hrs, 24 hrs and 1 week. The results revealed that epilepsy induction caused a significant inhibition of serum cholinesterase activity 3 hrs after induction while in the brain, the activity of cholinesterase was significantly increased after 24 hrs Serum glucose level was significantly elevated after 3 hrs and 24 hrs of induction, total cholesterol and phospholipids were not changed. From the results obtained in this study, it can be concluded that epilepsy caused significant changes in cholinesterase activity in brain and serum in addition to the glucose level in the serum.

  13. Effects of sodium ferrous chlorophyll treatment on anemia of hemodialysis patients and relevant biochemical parameters.

    Science.gov (United States)

    Xu, X F; Hu, J P; Cheng, X; Yu, G J; Luo, F; Zhang, G S; Yang, N; Shen, P

    2016-01-01

    This study explores the effects of sodium ferrous chlorophyll treatment on the anemia of maintenance hemodialysis (MHD) patients, as well as the relevant biochemical parameters. We selected 72 patients who had received regular MHD treatment two or three times a week for more than 3 months in the Hospital of Traditional Chinese Medicine of Zhengzhou City of Henan Province from March 2014 to March 2016. They were equally divided into a treatment group and a control group. Haemoglobin (HB) and hematocrit (HCT) of the treatment group increased significantly after treatment (p 0.05). Albumin (ALB) dosage of the treatment group increased after treatment (p 0.05). ALB level of the treatment group increased more significantly than in the control group (p MHD patients and their quality of life. PMID:27049084

  14. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss Fry

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    Salman Rauoof CHALKOO

    2013-01-01

    Full Text Available This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocytes (WBC and erythrocytes counts (RBC, hemoglobin (Hb content, hematocrits (PCV, leukocytes differential count and blood indices such as MCV, MCH and MCHC. Also blood serums were analysed for total protein (TP and blood enzymes. All the calculations were made using the SPSS© and t-test statistical method. In hematological findings nine parameters were revealed significant differences (P

  15. THE STUDY OF SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH MULTIPLE MYELOMA

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    Bularda Morozan Mihai

    2010-09-01

    Full Text Available This paper presents the results of some biochemical parameters obtained in the laboratory of “Elena Beldiman” Emergency Hospital Barlad, and in the”Dr. Stoian –Dr. Ungureanu”Private Medical Practice on 10 patients diagnosed with multiple myeloma during 2005-2011. Numerous researches over serum proteins with the help of various methods ( refractometry have shown the presence of some changes in protein fractions of blood serum. These changes express the change of the normal ratio between these fractions, that means disorders in colloidal structure of blood. The results of the electrophoresis analysis, also in accordance with those from the scholarly literature, show that the changes in the condition of the blood serum are due to the increase in the globulins fractions which is linked to the growth of the immunological processes, the antibodies synthesis being especially closely linked to gammaglobulinic fraction

  16. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

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    Sunita J Ramanand

    2014-01-01

    Full Text Available Background: Polycystic ovary syndrome (PCOS is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS. Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results : The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45. Hypoadiponectinemia (serum adiponectin <4 μg/mL was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027. Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56. It was significantly lower in patients with acanthosis nigricans (AN as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038. Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57. A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH, thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH, LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN of insulin resistance.

  17. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA) COLLECTED IN THE INACTIVE SEASON

    OpenAIRE

    AGRIPINA SAPCALIU; I. RADOI; D. CONDUR; A. SICEANU; ELIZA CAUIA; CRENGUTA PAVEL

    2013-01-01

    The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera) in inactive...

  18. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

    OpenAIRE

    AGRIPINA SAPCALIU; ELIZA CAUIA; ION GURESOAIE; DAN CONDUR; ION RADOI

    2013-01-01

    Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination). The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth...

  19. Analysis of some biochemical and haematological parameters for Mucuna pruriens (DC) seed powder in male rats.

    Science.gov (United States)

    Chukwudi, Ndukwe Henry; Simeon, Omale; Chinyere, Aguiyi John

    2011-10-01

    The biochemical and haematological effects of the seed powder of Mucuna pruriens in male rats were evaluated to establish some biological properties of this potential biopesticide currently undergoing investigation. The result showed that Mucuna pruriens seed extract produced a significant (p<0.05) increase in white blood cell (WBC) count, as well as in bilirubin concentrations, alkaline phosphatase (ALP), protein and creatinine levels measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly reduced (P<0.05) in comparison with the experimental control. PCV, Hb, albumin level and WBC differential counts gave no significant difference between treated and control groups. The results revealed metabolic imbalance in the rats which suggests a mild cholestasis effect of the extract. PMID:21959815

  20. Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil.

    Science.gov (United States)

    Anzolin, D G; Sarkis, J E S; Diaz, E; Soares, D G; Serrano, I L; Borges, J C G; Souto, A S; Taniguchi, S; Montone, R C; Bainy, A C D; Carvalho, P S M

    2012-07-01

    The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region. PMID:22626623

  1. Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil.

    Science.gov (United States)

    Anzolin, D G; Sarkis, J E S; Diaz, E; Soares, D G; Serrano, I L; Borges, J C G; Souto, A S; Taniguchi, S; Montone, R C; Bainy, A C D; Carvalho, P S M

    2012-07-01

    The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region.

  2. Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L.

    Science.gov (United States)

    Bagheri, Mahsa; Mansouri, Hakimeh

    2015-03-01

    This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. PMID:25492688

  3. Women with metabolic syndrome improve antrophometric and biochemical parameters with green banana flour consumption

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    Sandra Tavares da Silva

    2014-05-01

    Full Text Available Background: Consumption of green banana flour (GBF may promote health benefits, such as, decreased appetite, weight loss, glycemic control, intestinal function and lipid profile improvement, aging delay, cancer and heart disease prevention. The aim of this study was to evaluate the effects of green banana flour consumption on anthropometric and biochemical parameters in overweight women. Methods: The glycemic index of flour in the study was determined. The effects of consumption of 20 g of green banana flour/day on weight, body mass index (BMI, blood pressure, waist and hip circumference, body composition, hemoglobin, lipid profile, glucose, insulin, insulin resistance, liver function and energy intake were evaluated in 25 overweight women for 45 days. Results: The glycemic index of the flour under study was classified as low. Reduction (p < 0.05 in systolic blood pressure, hip circumference and fasting glucose levels were found in women who had metabolic syndrome criteria. Conclusions: Consumption of green banana flour (20 g/day for 45 days did not promote weight loss or changes in body composition in overweight women. It was noted, however, decreased hip circumference. Significant health parameter improvements were also noted in individuals with metabolic syndrome, which showed a reduction in systolic blood pressure and fasting glucose.

  4. The effects of Cosmos caudatus (Ulam Raja) supplementation on bone biochemical parameters in ovariectomized rats.

    Science.gov (United States)

    Mohamed, Norazlina; Yin, Chai Mei; Shuid, Ahmad Nazrun; Muhammad, Norliza; Babji, Abdul Salam; Soelaiman, Ima Nirwana

    2013-09-01

    Cosmos caudatus (ulam raja) contains high mineral content and possesses high antioxidant activity which may be beneficial in bone disorder such as postmenopausal osteoporosis. The effects of C. caudatus on bone metabolism biomarkers in ovariectomized rats were studied. 48 Sprague-Dawley rats aged three months were divided into 6 groups. One group of rats was sham-operated while the remaining rats were ovariectomized. The ovariectomized rats were further divided into 5 groups: the control, three groups force-fed with C. caudatus at the doses of 100mg/kg, 200mg/kg or 300mg/kg and another group supplemented with calcium 1% ad libitum. Treatments were given 6 days per week for a period of eight weeks. Blood samples were collected twice; before and after treatment. Parameters measured were bone resorbing cytokine; interleukin-1 and the bone biomarkers; osteocalcin and pyridinoline. Serum IL-1 and pyridinoline levels were significantly increased in ovariectomized rats. Supplementation of C. caudatus was able to prevent the increase of IL-1 and pyridinoline in ovariectomized rats. Besides that, C. caudatus showed the same effect as calcium 1% on biochemical parameters of bone metabolism in ovariectomized rats. In conclusion, Cosmos caudatus was as effective as calcium in preventing the increase in bone resorption in ovariectomized rats.

  5. Effect of ferrous sulphate on haematological, biochemical and immunological parameters in neonatal calves

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    Lobna S. Elgebaly

    2010-09-01

    Full Text Available The effect of oral administration of iron on haematological, biochemical and immunological parameters in neonatal calves was studied. Ten calves from a private farm in Gharbia Governorate were used. Calves were separated from their dams immediately after birth and received colostrum during the first hours after calving and twice daily for 48 h. Thereafter, they received whole milk. Calves were divided into two equal groups. The first group was kept as controls. Calves of the second group were given ferrous sulphate at a dose of 250 mg/calf daily, beginning at one day of age; this was continued for 28 days. Three blood samples were collected from each calf in all groups at 14, 21, 28 and 35 days of age. Iron administration produced a significant increase in red blood cell count, haemoglobin, packed cell volume and blood indices, in addition to non-significant changes in total and differential leukocyte counts. The administration of iron resulted in a significant increase in serum iron, total proteins, globulins, thyroid hormones, lymphocyte stimulation index, phagocytosis, body weight and body gain. The administration of iron is suggested as routine practice in calf-producing farms due to its advantageous effects on the parameters tested.

  6. Influence Of Feminine Hormones On Some Biochemical Parameters In Early And Late Menopausal Status

    International Nuclear Information System (INIS)

    Forty two healthy Egyptian women were participated in this study to evaluate the effect of early and late menopause on some biochemical and hormonal parameters. The women were divided into three equal groups. The first was the pre-menopausal group with regular menstrual cycle, the second was the late menopausal group comprised the menopausal female less than 5 years and the third group consisted of women that became menopause since 5-9 years. The second and third groups had almost the same age and body weight. After clinical examination, fasting blood samples were collected from all volunteers. Regarding the pre-menopausal women, blood was withdrawn between the 3rd and the 5th day post-menstruation (follicular phase). Follicle stimulating hormone (FSH), leutinizing hormone (LH), progesterone, prolactin (PRL), testosterone, leptin and thyroxine (T4) in addition to hemoglobin (Hb), blood glucose, calcium (Ca), inorganic phosphorous (P), magnesium (Mg) and uric acid were determined. Both menopausal groups showed significant increase in FSH and LH and significant decrease in PRL and late menopausal group experienced also multiple significant correlations between FSH, LH and other tested parameters. Prolongation of menopause exhibited decrease in leptin being significant as compared to pre-menopausal women than that experienced menopause at younger age. Due to the relation between leptin hormone and osteoporosis, the results of this investigation denoted that early cessation of annulations (menopause) in Egyptian women may lead to disturbance in bone metabolism causing inhibiting osteoblastogenesis and decreasing bone mass.

  7. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

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    Fabrícia Souza Predes

    2009-04-01

    Full Text Available This study was carried out to evaluate the effects of Arctium lappa L. (burdock on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST, alanine transaminase (ALT, gamma glutamyl transferase (GGT, total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.A bardana (Arctium lappa L é uma planta trazida do Japão e aclimatada no Brasil, e é extensamente utilizada na medicina popular em todo mundo. Este estudo foi realizado para avaliar os possíveis efeitos da A. lappa no fígado e nos parâmetros bioquímicos plasmáticos em ratos Wistar adultos. Estes receberam a infusão de bardana nas doses de 10 ou 20 g de folhas secas /L de água, por 40 dias. Não houve alteração significativa nos níveis plasmáticos de albumina, aspartato transaminase (AST, alanina transaminase (ALT, gamma glutamil transferase (GGT, proteínas totais, colesterol total, uréia, ácido úrico, triglicérides, cálcio, fósforo, bilirrubina direta e cloro. A análise morfológica não revelou alterações histopatológicas no fígado. Os dados bioquímicos e morfológicos não indicaram a toxicidade da bardana.

  8. Toxicity of Neem Leaf Extracts (Azadirachta indica A. Juss on Some Haematological, Ionoregulatory, Biochemical and Enzymological Parameters of Indian Major Carp, Cirrhinus mrigala

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    M. Saravanan

    2011-10-01

    Full Text Available In the present study, the median lethal concentration (LC 50 of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT and glutamate pyruvate transaminase (GPT activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in environmental biomonitoring of neem based products in freshwater environment.Keywords: Azadirachta indica, Acute toxicity, Cirrhinus mrigala, Haematology, Ion regulation, Biochemical and Enzymological parameters.

  9. Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men

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    Meng Rao

    2015-01-01

    Full Text Available In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below, motility (P = 0.009 and 0.021, respectively, the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively, total acrosin activity (P = 0.018 and 0.009, respectively, and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively. The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031. We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

  10. Biochemical brain markers and purinergic parameters in rat CSF after seizure induced by pentylenetetrazol.

    Science.gov (United States)

    Oses, Jean Pierre; Leke, Renata; Portela, Luis Valmor; Lara, Diogo R; Schmidt, André P; Casali, Emerson André; Wofchuk, Susana; Souza, Diogo O; Sarkis, João José Freitas

    2004-09-30

    Cellular and molecular mechanisms involved in the generation of seizures and the magnitude of neural cells injury are not fully understood. We evaluated astrocyte and/or neuronal injury in rats in the pentylenetetrazol model of acute seizures by measuring S100B and NSE levels in cerebrospinal fluid. Additionally, we determined ADP and GDP hydrolysis by soluble nucleoside triphosphate diphosphohydrolase in the cerebrospinal fluid, and the concentration of nucleosides adenosine, inosine and guanosine as putative markers of brain injury. After pentylenetetrazol-induced seizures: (i) S100B values increased from 10 to 30 min, returning to control levels at 24 h; NSE levels presented a biphasic increase: an increase at 10 to 30 min returning to control levels, and again at 240 min followed by a decline at 24 h; (ii) nucleotidase activities increased from 10 min, returning to control levels at 240 min; (iii) guanosine and inosine levels increased exclusively after 30 min. In summary, this study showed biochemical changes in the cerebrospinal fluid occurring after seizures induced by pentylenetetrazol. Such events may have a modulating effect upon seizure expression, particularly nucleoside triphosphate diphosphohydrolase activities and nucleoside concentrations, but are nevertheless followed by neural death as evidenced by the increase in NSE and S100B levels.

  11. Effects of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854)

    Science.gov (United States)

    Zhu, Zhiming; Song, Bolan; Lin, Xiaotao; Xu, Zhongneng

    2016-01-01

    This study examines the effect of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb ( Barbonymus schwanenfeldii). Blood samples were taken on days 1, 23 and 45 from control fish and from two training groups maintained at current speeds of 0.06 bl/s (body length per second), 0.66 bl/s, and 1.92 bl/s, respectively. Significantly increased red-blood-cell counts and hematocrit were observed in the post-training groups on days 23 and 45. Significantly increased hemoglobin concentrations were also observed in the 1.92 bl/s group on days 23 and 45. In contrast, values of mean corpuscular volume were significantly lower in the 1.92 bl/s group than in the other groups on day 45. Nitroblue-tetrazolium-positive cells and lysozyme and superoxidase dismutase activities in the plasma increased significantly with increasing training intensity on days 23 and 45. Antibacterial activities were significantly increased in the trained groups compared with the control group on day 23; significantly elevated alkaline phosphatase activity was observed in the 1.92 bl/s groups on day 45. Therefore, training intensities of 0.66 and 1.92 bl/s enhanced the blood oxygen-carrying capability and plasma immune parameters of juvenile tinfoil barbs.

  12. Assessment of hemato-biochemical parameters on exposure to low level of deltamethrin in mouse model

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    Anita Tewari

    2014-03-01

    Full Text Available Aim: In this study, sub-acute toxicity of deltamethrin on hematological and biochemical blood parameters of male albino Swiss mice was evaluated. Materials and Methods: Generally, the maximum permissible residue level (MRL of deltamethrin for food products lies between 0.01 to 0.5 mg/kg body weight. So the mice were exposed orally with two doses of pesticide i.e. 0.1 and 0.5 mg/kg body weight. The doses were given on a daily basis for a period of 15 days and 30 days respectively. Ground nut oil was used as control treatment. Samples of blood were collected at the end of the treatment. Hepatotoxicity was evaluated by quantitative analysis of the serum enzymes alanine transaminase (ALT, aspartate transaminase (AST, alkaline phosphatase (ALKP, total bilirubin (TBIL and serum urea. Alterations of hematological parameters were analysed by total leukocyte, differential leukocyte count and hemoglobin levels. Results: Significant increase in the levels of hepatic enzymes (ALT, AST, ALKP were observed for both doses, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations for 0.5 mg/kg body weight dose which is indicated by leukocytosis, lymphocytosis and neutropenia in long duration study. Conclusions: The results indicated that even very low dose of deltamethrin can promote hematological and hepatic alterations. Thus it is imperative to do further studies on the detrimental effect of the low levels of pyrethroid commonly present in our food, which further necessitate the reduction of maximum permissible levels of residual synthetic pyrethroid levels in foods and feed.

  13. Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

    Energy Technology Data Exchange (ETDEWEB)

    Smutna, M.; Hilscherova, K.; Paskova, V. [Masaryk Univ., Brno (CZ). RECETOX (Research Centre for Environmental Chemistry and Ecotoxicology); Marsalek, B [Czech Academy of Science, Brno (Czech Republic). Centre for Cyanobacteria and their Toxins

    2008-06-15

    Background, aim, and scope Restoration of lakes and reservoirs with extensive cyanobacterial water bloom often requires evaluation of the sediment quality. Next to the chemical analysis of known pollutants, sediment bioassays should be employed to assess toxicity of the present contaminants and to make predictions of associated risk. Brno reservoir in the Czech Republic is a typical example of water bodies with long-term problems concerning cyanobacterial water blooms. Comprehensive assessment of reservoir sediment quality was conducted since successful reservoir restoration might require sediment removal. An important part of this survey focused on an examination of the utility of Tubifex tubifex and its sublethal biochemical markers for the assessment of direct sediment toxicity. Materials and methods This complex study included chemical analysis of contaminants (heavy metals, organic pollutants), ecotoxicity testing of sediment elutriates (tests with Daphnia magna, Pseudomonas putida, Sinapis alba, Scenedesmus subspicatus), and other parameters. We have tested in more detail the applicability of T. tubifex as a test organism for direct evaluation of contact sediment toxicity. Survival tests after 14 days of exposure were complemented by an assessment of parameters serving as biomarkers for sublethal effects [such as total glutathione content (GSH), activities of the enzymes glutathione transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR)]. The data matrix was subjected to multivariate analysis to interpret relationships between different parameters and possible differences among locations. Results The multivariate statistical techniques helped to clearly identify the more contaminated upstream sites and separate them from the less contaminated and reference samples. The data document closer relationships of the detected sediment contamination with results of direct sediment exposure in the T. tubifex test regarding mortality but namely

  14. Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters

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    Rithiele Gonçalves

    2014-11-01

    Full Text Available Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT, glutamic pyruvic transaminase (GPT, creatine-kinase (CK, creatine kinase-muscle B (CK-MB and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days, respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01, while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01. Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain, carbonyl (plasma and brain and micronucleus compared to the saline-group (p ≤ 0.01. Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.

  15. Sublethal concentrations of di-n-butyl phthalate promote biochemical changes and DNA damage in juvenile Nile tilapia (Oreochromis niloticus).

    Science.gov (United States)

    Khalil, Samah R; Abd Elhakim, Yasser; El-Murr, Abd Elhakeem

    2016-02-01

    Increase in consumption of consumer items such as plasticizers have resulted in a sharp rise in the presence of xenobiotics like phthalic acid esters (PEs) in freshwater and marine environments due to contaminated runoff and improper release of effluents. The sublethal toxicity of Di-n-butyl phthalate (DBP) was investigated in juvenile Nile tilapia, Oreochromis niloticus, in an attempt to determine the biological effect of exposure to 1/2 and 1/3 median lethal concentration (96-h LC50) which, in our study was experimentally determined to be 11.8 mg/l. Following four days of exposure, indices of the oxidative potential [Malondialdehyde content (MDA)], antioxidant parameters [superoxide dismutase activity (SOD) and reduced glutathione level (GSH)] and DNA damage were evaluated by single-cell gel electrophoresis (Comet assay). Hepato-renal markers [alanine aminotransferase activity (ALT), creatinine and urea level] and cortisol levels were also quantified in serum. Additionally, histopathological investigations of liver, kidney and gill tissues were conducted. Comparative results between the 1/2 96-h LC50 group and the 1/3 96-h LC50 group clearly showed that there was a significant elevation in MDA levels and a marked increase in DNA damage in addition to inhibition of antioxidant barriers as represented by attenuation of SOD activity and GSH level in the group that was exposed to higher concentration of DBP (1/2 96-h LC50). The hepatorenal markers and cortisol levels were also observed to be elevated. Histopathological examination of the liver, kidney and gills showed pathological alterations that could be correlated with changes in the biochemical profile of the exposed fish. Additionally, anomalous clinical signs were noted. Based on these findings, we conclude from our study that exposure of juvenile O. niloticus to DBP has the potential to induce biochemical as well as tissue morphological alterations associated with oxidative injury and DNA damage. PMID:27348890

  16. Effect of Gundelia tournefortii on some biochemical parameters in dexamethasone-induced hyperglycemic and hyperlipidemic mice

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    O. H. Azeez

    2012-01-01

    Full Text Available The aim of this study was conducted to evaluate the effect of Gundelia tournefortii on some biochemical parameters in hyperglycemic and hyperlipidemic mice. Male albino mice were induced hyperglycemic and hyperlipidemic by daily injection of dexamethasone 1 mg/kg of body weight intramuscularly (i.m., the mice randomly divided into five groups (6-8 mice in each group. The group 1: served as negative control group; the group 2: injected with dexamethasone at dose 1 mg /kg.b.w.i.m and served as positive control group; the groups 3, 4, 5: treated with extract of G. tournefortii at doses: 75, 150, 300 mg/kg.b.w. orally respectively companied with injection of dexamethasone 1 mg/kg.b.w.i.m. All treatment were once daily for 22 days. Dexamethasone treatment lead to significant increase in levels of glucose, cholesterol, and triglyceride, and significant decrease of body weight, without any effect on level of total protein. G. tournefortii extract treatment at doses: 75 mg/kg.b.w. resulted significant decrease levels of glucose, and body weight. Beneficial effect were seen when mice treated with G. tournefortii at dose of 300 mg/kg.b.w. that lead to significant decrease in levels of glucose, triglyceride, and cholesterol. These results indicate the usefulness of G. tournefortii extract as hypoglycemia and hypolipidemia in dexamethasone treated mice.

  17. Protective role of wheat germ oil on some biochemical parameters in irradiated rats

    International Nuclear Information System (INIS)

    Wheat germ oil is an organic nutritionally rich vegetable oil. It is an excellent source of vitamin E and essential fatty acids (octacosanol, linoleic and linolenic). The study confirmed the beneficial role of the used anti-oxidation agents as recommended radio-protectors due to their ability of scavenging free radicals produced by ionizing radiation. The efficacy of daily oral treatment of rats with wheat germ oil (10 mg/ Kg body wt) for 15 days to control many of the damaging effects of ionizing radiation when male rats were subjected to fractionated 8 Gy (2 Gy day after day) of gamma irradiation were studied. Blood samples were collected from animals at 10 and 15 days after treatment and/or exposure. In blood, the data obtained revealed that, radiation exposure caused significant increases in levels of malondialdehyde (MDA), triacylglycerol (TG), total cholesterol (TC), low-density lipoproteins (LDL) and glucose at the 10th day. Meanwhile, significant reduction in contents of total protein, albumin, globulins, high-density lipoproteins (HDL), vitamin E and glucose were recorded at the 15th day. Also, the majority of these parameters were estimated in liver tissues. The results revealed that administration of the natural product wheat germ oil partially ameliorated the radiation-induced biochemical disturbances. These effects were explained in the light of the presumed different mechanisms of wheat germ oil actions

  18. Effect of gamma irradiation on ripening, storage and biochemical parameters of banana (Musa paradisiaca L.)

    International Nuclear Information System (INIS)

    Banana (Musa paradisiaca L.) popularly known as kadali (S) belongs to the family Musaceae is an economically important plant due to its nutritive fruit used as staple food all over the world. Several techniques have been employed to delay fruit ripening and softening by storing preclimacteric bananas in modified or controlled atmospheres. The Food and Drug Administration (FDA) has recommended the usage up to 1.0 kGy of gamma irradiation for delaying the ripening in fruits. Irradiation significantly improved shelf life by inhibiting the fungal growth in strawberries. Hence, present work was undertaken to scrutinize the impact of gamma irradiation on the ripening, storage and the biochemical attributes of Musa paradisiaca L. variety kadali. Present work revealed that irradiation at 0.2 kGy and 0.3 kGy retained their organoleptic properties till 16th day of storage against under normal conditions. Nutritional parameters such as ascorbic acid content, soluble sugar and protein contents were significantly higher in irradiated fruits. Hence, lower dose of gamma irradiation could be successfully used in extending the shelf life and nutritional quality and delay the process of ripening in Musa paradisiaca L.

  19. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA COLLECTED IN THE INACTIVE SEASON

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    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera in inactive season. The researches were carried out on honeybee samples collected from 5 honeybee colonies belonging to a breeding apiary of the Institute for Beekeeping Research and Development from Bucharest. In order to perform the biochemical analyses, the honeybees samples, consisting in 50 individuals on sample (10 individuals/colony were randomly collected and their haemolimph collected, at different time intervals, in inactive season (fall-winter. Totally, there were collected 250 haemolyph samples in a 2 years interval and the following 21 biochemical parameters were analysed: GLU, HDL-c, ALP, T-cho, Tprot, Alb., BUN, LDH, CPK,, Mg, IP, GGT, GOT, GPT, Ca, Cre,, Amy, T–BIL, TG, UA.. The test was carried out after the collection and processing of the samples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique, respectively .During the 2nd part of the inactive season, the values of most biochemical parameters increase in different proportions, their levels being maintained also in the first part of the active seasons (April, May, June.The values obtained for the main studied biochemical parameters in the haemolymph of the healthy honeybees collected from honeybee colonies kept in natural conditions show a highly variable evolution in the two consecutive years of experiments during the inactive season.

  20. Influence of Dietary Irradiated Curcuma ionga (Turmeric) on Physiological and Biochemical Parameters of Growing Rats

    International Nuclear Information System (INIS)

    Turmeric, (Curcuma ionga), is a dietary antioxidant and has been known since ancient times to possess therapeutic properties. The present investigation examined the correlation between raw and irradiated turmeric powder (at dose levels of 10, 15 and 20 kGy) intake and the physiological effects on organs weight, haematological parameters, indices of liver function and lipid profile in growing Albino male rats. Also, this study was performed to examine the efficacy of radiation processed turmeric powder to modulate the induced hyperlipidaemia in growing Albino male rats. Thirty six male rats were equally and randomly categorized into six groups. Control group fed a reference diet (casein diet), high fat diet was daily received to rats for 6 weeks. Other animals fed daily on high fat diets containing either raw or irradiated turmeric powder (2 g per 100 g diet) at doses 10, 15 or 20 kGy for 6 weeks. The results showed that the relative spleen, kidneys, heart, lungs, and testes weight, and the levels of Hb, PCV and MCHC were not changed in animals fed the experimental diets for 6 weeks. Animals kept on HFD suffered from liver enlargement. Dietary interventions through administrating 2% (w/w) raw or irradiated turmeric powder at 20 kGy normalized the liver size as compared with those received reference diet. Meanwhile, the results revealed that rats fed on high fat diet significantly increased plasma AST, ALT, TC and TG and significant decrease was observed in HDL. Meanwhile, feeding rats on diet containing of either raw or irradiated turmeric powder at 10, 15 and 20 kGy induced a significant improvement in the above mentioned parameters. These results imply that irradiated turmeric powder at 20 kGy can offer protection against the biochemical changes as a consequence of hyperlipidaemic food and contribute to the regulation of lipid metabolism safely

  1. EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS

    Science.gov (United States)

    EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS. JP Nolan1, LB Wichers2, J Stanek3, UP Kodavanti1, MCJ Schladweiler1, PA Evansky1, ER Lappi1, DL Costa1, and WP Watkinson1...

  2. Effect of wood ash application on the morphological, physiological and biochemical parameters of Brassica napus L.

    Science.gov (United States)

    Nabeela, Farhat; Murad, Waheed; Khan, Imran; Mian, Ishaq Ahmad; Rehman, Hazir; Adnan, Muhammad; Azizullah, Azizullah

    2015-10-01

    The present study was conducted to determine the effect of wood ash application on different parameters of Brassica napus L. including seed germination, seedling growth, fresh and dry biomass, water content in seedlings, photosynthetic pigments, soluble sugars, total protein and cell viability. In addition, the effect of wood ash on soil microflora and accumulation of trace elements in seedlings were determined. The seeds of B. napus were grown at different doses of wood ash (0, 1, 10, 25, 50 and 100 g (wood ash)/kg (soil)) and the effect on various parameters was determined. Wood ash significantly inhibited seed germination at doses above 25 g/kg and there was no germination at 100 g/kg of wood ash. At lower concentrations of wood ash, most of the growth parameters of seedlings were stimulated, but at higher concentrations of wood ash most of the studied parameters were adversely affected. Wood ash was found to be very detrimental to B. napus when applied above 25 g/kg. Wood ash application resulted in an increased bioaccumulation of trace elements in seedlings of B. napus. Almost all trace elements were significantly higher in seedlings grown in wood ash above 10 g/kg as compared to the control. An increase in total microbial count was observed with wood ash treatment which was statistically significant at 1 and 10 g/kg of wood ash. It is concluded that at very high concentration, wood ash can be detrimental to plants; however, its application at lower application rate can be recommended. PMID:26163419

  3. White popular (Populus alba L.) - Litter impact on chemical and biochemical parameters related to nitrogen cycle in contaminated soils

    Energy Technology Data Exchange (ETDEWEB)

    Ciadamidaro, L.; Madejon, P.; Cabrera, F.; Madejon, E.

    2014-06-01

    Aim of study: The aim of this study was to determine the effect of litter from Populus alba on chemical and biochemical properties related to the N cycle in soils with different pH values and trace element contents. We hypothesized that this litter would influence several parameters related to the N cycle and consequently to soil health. Area of study: we collected two reforested contaminated soils of different pH values (AZ pH 7.23 and DO pH 2.66) and a non-contaminated soil (RHU pH 7.19). Materials and methods: Soil samples were placed in 2,000 cm{sup 3} microcosms and were incubated for 40 weeks in controlled conditions. Each soil was mixed with its corresponding litter, and soils without litter were also tested for comparison. Ammonium (NH{sub 4}{sup 4}+-N) and nitrate (NO{sub 3}{sup -} -N) content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN), protease activity, and several chemical properties such as pH, available trace element concentrations (extracted with 0.01 M CaCl{sub 2}) were determined at different times of incubation. Main results: Values of available trace elements did not vary during the incubation and were always higher in acid soil. In neutral soils litter presence increased values of Kjeldahl-N, NO{sub 3} –-N content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN) and protease activity. Presence of trace elements in neutral soils did not alter the parameters studied. However, acidic pH and high content of available trace elements strongly affected NH{sub 4}{sup +}-N and NO{sub 3}{sup -} -N, microbial biomass N and protease activity. Research highlights: Our results showed the negative effect of the acidity and trace element availability in parameters related with the N-cycle. (Author)

  4. Changes In Cortisol And Some Biochemical Parameters In Blood Of Egyptian Buffalo Calves Exposed To Two Successive Summer Seasons

    International Nuclear Information System (INIS)

    Ten male buffalo calves were used in the present study. The animals were housed in semi-open barn. Ration was composed of concentrate mixture and wheat straw according to NRC (1985). Animals were allowed to drink fresh water ad libitum. Plasma cortisol, total protein, albumin, globulin, urea, creatinine, GPT and GOT were determined at the end of thermoneutral and summer heat stress exposure periods. Rectal temperature and respiration rate were recorded daily during both periods whereas body weight was recorded weakly during each period. Summer heat stress induced significant increases in rectal temperature, respiration rate, plasma cortisol, albumin/globulin ratio, plasma urea-N, creatinine, GPT and GOT while total protein, albumin, globulin did not affect by summer heat stress. Body weight was decreased under heat stress in both summer seasons but increased with age. In conclusion, summer heat stress induced alterations in some physiological and biochemical parameters of blood and body weight of male buffalo calves, and the young calves were less tolerant to summer heat stress than the older calves.

  5. Effect of Different Growth Conditions on Certain Biochemical Parameters of Different Cyanobacterial Strains

    Directory of Open Access Journals (Sweden)

    Hammouda, O. E.

    2012-01-01

    Full Text Available Aims: Variation in the traditional growth medium conditions to enhance the production of lipids, carbohydrates, protein and the free amino acids content of three cyanobacterial species. Methodology and Results: Three species of cyanobacteria (Anabaena laxa, Anabaena fertilissima and Nostoc muscorum were collected from the culture collection of Soils, Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt, to investigate their biochemical composition under different growth conditions, using BG110 (nitrogen free as growth medium. These conditions were represented by control medium, static glucose medium with (1%, w/v, aerated medium (aerated by bubbling technique depending on CO2 normally existed in air with a concentration of 0.03%, molasses medium (0.7%, v/v and aerated medium enriched with glucose (1%, w/v. Lipid content, total carbohydrates, soluble proteins and free amino acids were determined at the previous conditions. Glucose at 0.7% (w/v was the most favorable for lipid production in A. laxa, where it exhibited the highest lipid content (427 μg/g fresh wt.. Increasing molasses concentration up to 0.7% (v/v produced an increase in lipid contents of the tested cyanobacterial strains. The highest lipid content of both N. muscorum (366.2 μg/g fresh wt. and A. laxa (357.4 μg/g fresh wt. were recorded at molasses concentrations of 0.1 and 0.7% (v/v, respectively. A. laxa expressed high significant values for both proteins (31.6 μg/mL and free amino acids (40.5 mg/g dry wt. after 6 days of incubation period under aerated enriched glucose condition (1%, w/v. Also, at the same growth conditions, A. fertilissima exhibited high significant values for carbohydrates at 4th day (876.8 mg/g dry wt.. Conclusion, significance and impact of study: Aerated enriched glucose medium (1%, w/v was the best growth medium condition used in the present study.

  6. INFLUENCE OF PULSED SHORT WAVE (DIAPULSE TREATMENT ON THE BIOCHEMICAL PARAMETERS CORTISOL AND ALDOSTERONE

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    Dogaru Gabriela

    2015-12-01

    Full Text Available Pulsed short waves are part of high frequency therapy; unlike continuous short waves, where the phenomenon of tissular endothermia occurs, they have therapeutic effects that seem to depend rather on the interaction of electromagnetic waves with biological tissue, as they act only through specific electromagnetic energy. The recommended work technique for the use of the Diapulse device is that regardless of the exposed somatic region, there will be an initial exposure of one of the following regions: prehepatic, epigastric or lumbar (for action on the adrenal glands. Considering the role and the effects of the hormones of the adrenocortical gland, particularly glucocorticoids, on the body, which influence the inflammatory response of the connective tissue, with a role in rheumatoid pathology, we monitored the changes in the function of this gland. The biochemical parameters cortisol and aldosterone were quantitatively assessed in patients with complex regional pain syndrome type I (algoneurodystrophy treated with pulsed short waves, who were initially exposed to radiation of the lumbar region. In the same treatment session, all patients were exposed to radiation of the lumbar region, at a dose of 4/400 impulses/sec., for 10 minutes, followed by radiation of the affected region at a dose of 6/600 impulses/sec., for 10 minutes. There was one treatment session per day, for 14 days. It was demonstrated that radiation of the lumbar region at a dose of 4/400 impulses/sec. caused no changes in the hormonal function of the adrenal gland, i.e. no hypo- or hyperfunction; there was no overstrain or overstress after exposure to pulsed short waves, which is an important aspect, with applicability in clinical practice. Studies demonstrated both the local and the general, systemic influence of pulsed short waves, the induced changes being for the most part strictly dependent on the dose used.

  7. Effect Of Some Herbal Medicine On Some biochemical parameters In Diabetic Rats

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    Eman G.E. Helal, Mervat A.Abbas

    2006-03-01

    Full Text Available The current study was planned to follow up the effect of famous mixture used as a hypoglycemic traditional medicine in Saudi Arabia on diabetic albino rats and their effects on some biochemical parameter as DHEA .This study also aimed to study the effect of each plants alone to illustrate the most powerful one of them. The present study was carried out on eighty adult male albino rats (120+20gm b.wt. They were randomly divided into eight groups. First group conserved as control group.The others groups received alloxan to become diabetic .The second group conserved as diabetic, the third group treated with a mixture of plants (0.5gm/120gm b.wt.From he fourth to eight group,the rats treated with Nigella sativa (0.25gm/120gm b.wt,Aloe vera,Ferulaassa-foetid,Boswellia Carterii Birdw and Commiphora myrrha respectively (0.5gm/120gm b.wt..Our result showed that the body weight was reduced significantly in diabetic group and Nigella sativa treated group. On the other hand, treatment with commiphora myrrha revealed significant increase in body weight gain, while mixture and Aloe vera treated rats recorded insignificant change. Diabetic rats revealed a significant decrease in serum glucose and DHEA-S levels and liver glycogen content, while insignificant difference was recoded in all treated groups. A significant decrease in serum insulin level was observed in diabetic group, Nigella sativa and Ferula assa-foetida treated groups, but insignificant different was recorded in the rest treated groups throughout the experiment. A significant increase in LDH activities in diabetic and Ferula assa-foetida treated groups was recorded while rest treated groups recorded insignificant change During experimental period.

  8. The effects of Ramadan fasting and physical activity on body composition and hematological biochemical parameters

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    Seyyed Reza Attarzadeh Hosseini

    2014-09-01

    Full Text Available Introduction: Hunger and reduction in regular energy intake can lead to a number of problems based on their intensity. For instance, low energy level can cause blood cell production to decline or it may pose a higher risk of anemia. It can also weaken the immune system and platelet aggregation or negatively affect clot formation. This study aimed to have a closer look at fasting and regular physical activity and their impacts on body composition and blood hematological-biochemical parameters among professional wrestlers. Method: In this semi-experimental study, 9 subjects were selected by convenience sampling. The selected training program included participation in this exerciseprogram, 90 min per session, 6 times per week for a period of one month. Blood samples were obtained four times: before the start of Ramadan, 2 weeks after the start, during the last week and 2 weeks after the end of Ramadan. To make intra-group comparison, repeated measure analysis of variance was used. For all statistical comparisons, the level of significance was considered at PResults: Body weight and red blood cell count (RBC dropped significantly at the end of Ramadan (Respectively P= 0.001 and P=0.034. However, the number of white blood cells (WBC and circulating platelets (PLT significantly increased during fasting (Respectively P= 0.048 and P=0.042. As a matter of fact, PLT and WBC were the only factors which dramatically increased during fasting. Intra-group variations of tetracycline (TC, low-density lipoprotein (LDL, LDL: high-density lipoprotein (HDL, triglyceride (TG: HDL and TC: HDL reduced at the end of Ramadan. However, HDL levels ​​drastically increased during fasting (P≤0.05. Conclusion: Based on the results of the research, despite being a regular activity and fasting has beneficial effects on lipid profile in athletes, however, they can with tangible changes in hematological factors may lead to weaken the immune system of athletes.

  9. Lymphocytic Thyroiditis – is cytological grading significant? A correlation of grades with clinical, biochemical, ltrasonographic and radionuclide parameters

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    Dash Radharaman

    2007-01-01

    Full Text Available Abstract Background Clinical, biochemical, ultrasonographic, radionuclide and cytomorphological observations in Lymphocytic thyroiditis (LT, to define the cytological grading criteria on smears and correlation of grades with above parameters. Methods This prospective study was conducted on 76 patients attending the Fine needle aspiration cytology clinic of a tertiary care institute in North India. The various parameters like patients' clinical presentation, thyroid antimicrosomal antibodies, hormonal profiles, radionuclide thyroid scan and thyroid ultrasound were studied. Fine needle aspiration of thyroid gland and grading of thyroiditis was done on smears. The grades were correlated with above parameters and the correlation indices were evaluated statistically. Results Most of the patients were females (70, 92.11% who presented with a diffuse goiter (68, 89.47%. Hypothyroid features (56, 73.68% and elevated TSH (75, 98.68% were common, but radioiodide uptake was low or normal in majority of patients. Thyroid antimicrosomal antibody was elevated in 46/70 (65.71% patients. Cytomorphology in fine needle aspirates was diagnostic of lymphocytic thyroiditis in 75 (98.68% patients. Most of them had grade I/II disease by cytology. No correlation was observed between grades of cytomorphology and clinical, biochemical, ultrasonographic and radionuclide parameters. Conclusion Despite the availability of several tests for diagnosis of LT, FNAC remains the gold standard. The grades of thyroiditis at cytology however do not correlate with clinical, biochemical, radionuclide and ultrasonographic parameters.

  10. Impacts of Elevated CO2 Concentration on Biochemical Composition,Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae

    Institute of Scientific and Technical Information of China (English)

    Jian-Rong XIA; Kun-Shan GAO

    2005-01-01

    To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations,Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2concentrations within the range 3-186 μmol/L and the biochemical composition, carbonic anhydrase (CA),and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate,and protein contents were enhanced to varying extents with increasing CO2 concentration from 3-186μmol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186 μmol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent.

  11. Assessing seasonality of biochemical CO2 exchange model parameters from micrometeorological flux observations at boreal coniferous forest

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    T. Vesala

    2008-12-01

    Full Text Available The seasonality of the NEE of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the biochemical model parameters. Our study sites consisted of three Scots pine (l. Pinus sylvestris forests and one Norway spruce (l. Picea abies forest that were located in Finland and Sweden. We obtained temperature and seasonal dependence for the biochemical model parameters: the maximum rate of carboxylation (Vc(max and the maximum rate of electron transport (Jmax. Both of the parameters were optimized without assumptions about their mutual magnitude. The values obtained for the biochemical model parameters were similar at all the sites during summer time. To describe seasonality, different temperature fits were made for the spring, summer and autumn periods. During summer, average Jmax across the sites was 54.0 μmol m−2 s−1 (variance 31.2 μmol m−2 s−1 and Vc(max was 12.0 μmol m−2 s−1 (variance 6.6 μmol m−2 s−1 at 17°C. The sensitivity of the model to LAI and atmospheric soil water stress was also studied. The impact of seasonality on annual GPP was 17% when only summertime parameterization was used throughout the year compared to seasonally changing parameterizations.

  12. Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites

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    Wang Yongwei

    2012-12-01

    Full Text Available Abstract Ascites syndrome is still a problem for chicken industry in various parts of the world. Despite the intensive investigations of this syndrome for many years, its pathogenesis remains unclear. The objective of this study was to analyze the difference in hepatic proteomics between ascites and healthy broilers by two-dimensional electrophoresis (2-DE and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS. Changes of biochemical parameters of liver and blood were also determined. The results indicated that red blood cell counts (RBC, hematocrit (HCT and haemoglobin (HGB of ascites broilers were significantly greater than healthy broilers. Hepatic malondialdehyde (MDA level of ascites broilers was significantly increased, and the activity of total superoxide dismutase (T-SOD was significantly decreased. Hepatic lactic acid (LD level of ascitic broilers were significantly lower than healthy ones. Serum glucose and cholesterol level of ascites broilers were significantly increased, and serum globulin level was significantly decreased in ascites broilers. There was no significant difference in triglyceride (TG and blood urea nitrogen (BUN level. The activity of liver hexokinase (HK and succinodehydrogenase (SDH in ascites broilers was significantly decreased, and there was no significant difference in the activity of liver pyruvate kinase (PK and Na+-K+-ATPase. The hepatic proteomics analysis showed that 18 proteins expression difference were identified between ascites and healthy broilers. These proteins were mainly involved in: 1 cytoskeleton; 2 glucose, lipids and amino acid metabolism; 3 cell secretion; 4 cell apoptosis; 5 signal transduction; 6 immune and inflammatory response; and 7 cellular redox homeostasis. Mitochondrial isoform phosphoenolpyruvate carboxykinase (M-PEPCK mainly participates in gluconeogenesis of chicken liver. In conclusion, liver oxidative damage was significantly aggravated

  13. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2014-08-01

    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint ( P > 0.05). A significant interaction between dietary CrPic and P.mint on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P birds fed the CrPic-included diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  14. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2014-08-01

    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint ( P > 0.05). A significant interaction between dietary CrPic and P.mint on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P diet including both CrPic and P.mint. Plasma chromium (Cr) content increased significantly ( P diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  15. Investigation on some biochemical parameters and effect of hormonal treatment in anoestrous dairy cows with cystic ovarian follicle

    Institute of Scientific and Technical Information of China (English)

    StanimirA Yotov; AnatoliS Atanasov; GeorgiB Georgiev; JulietaD Dineva; NadejdaA Palova

    2014-01-01

    Objective:To investigate some blood biochemical parameters in anoestrous dairy cows with cystic ovarian follicles(COF) during the first two months post partum and the effect of hCG-PGF2α-GnRH treatment on their reproductive response.Methods:The investigation was performed on20 lactatingHolstein cows, divided into two groups: control group(n=8) and treated group (n=12).All animals had cystic ovarian follicles, detected during the two consecutive transrectal ultrasound examinations at7-day interval.Blood samples from each animal were collected on the day of second ultrasonography(Day0) and subjected to a hormonal and biochemical analysis. Treated cows were subjected to another ultrasound examination on day of prostaglandin injection and the presence of corpus luteum was recorded.The control animals were not treated, while the treated group received1500IU hCG on day0,PGF2α500 mg on day7 and100 μgGnRH 48 hours latter.Eighteen hours afterGnRH administration animals bearing corpus luteum on Day7 were artificiallly inseminated.The last ultrasound examination was made forty days after start of the threapy.On the base of the obtained results, cumulative oestrous activity, cumulative ovulation rate, pregnancy rate andCOF persistence were determined.Results:The blood analysis in the both groups onDay0 showed significantly(P<0.05) lower mean concentration of progesterone, glucose and inorganic phosphate compared to the reference range values for cyclic dairy cows.Cumulative oestrous activity(91.7%) and cumulative ovulation rate(75.0%) were considerably higher(P<0.05) in treated group than control group, whereas the cystic follicle persistence(25.0%) was lower(P<0.05) in the treated group than control group.Conclusions:The present study confirmed the thesis for low blood levels of glucose and inorganic phosphate in cows presenting cystic ovarian follicles.It also indicated significant improvement of cumulative oestrous activity and ovulation rate in animals subjected toh

  16. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation

    OpenAIRE

    Mishra, Arun K.; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with s...

  17. Effects of 24 h ultra-marathon on biochemical and hematological parameters

    Institute of Scientific and Technical Information of China (English)

    Huey-June Wu; Kung-Tung Chen; Bing-Wu Shee; Huan-Cheng Chang; Yi-Jen Huang; Rong-Sen Yang

    2004-01-01

    AIM: To analyze detailed changes in hematology and biochemistry tests parameters before and after a longdistance race in ultramarathon runners. METHODS: Blood samples of 11 participants were obtained for standard analysis before, immediately after, two days after and nine days after the 2002 International Ultramarathon 24 h Race and the International Association of Ultrarunners (IAU) Asia 24 h Championship. RESULTS: Total bilirubin (BIL-T), direct bilirubin (BIL-D), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased statistically significantly (P<0.05) the race. Significant declines (P<0.05) in red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) were detected two days and nine days d after the race. 2 d after the race, total protein (TP), concentration of albumin and globulin decreased significantly. While BIL, BIL-D and ALP recovered to their original levels. High-density lipoprotein cholesterol (HDL-C) remained unchanged immediately after the race, but it was significantly decreased on the second and ninth days after the race.CONCLUSION: Ultra-marathon running is associated with a wide range of significant changes in hematological parameters, several of which are injury related. To provide appropriate health care and intervention, the man who receives athletes on high frequent training program high intensity training programs must monitor their liver and gallbladder function.

  18. Antioxidant status and selected biochemical parameters of porcine ovarian granulosa cells exposed to lead in vitro.

    Science.gov (United States)

    Capcarová, Marcela; Kolesárová, Adriana; Lukác, Norbert; Sirotkin, Alexander; Roychoudhury, Shubhadeep

    2009-12-01

    The objective of this study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) and release of calcium, phosphorus, magnesium, sodium, potassium, total lipids, totals proteins, glucose, cholesterol and triglycerides by porcine ovarian granulosa cells cultured in vitro after lead acetate administration. The parameters were analyzed using semi-automated clinical chemistry analyzer Microlab 300, microprocessor-controlled analyzer EasyLite and spectrophotometer Genesys 10. Cells were cultured with lead acetate trihydrate [Pb(CH(3)COO)(2).3H(2)O] as follows: group Max (5 mg Pb(CH(3)COO)(2).3H(2)O/10 mL), group A (2.5 mg/10 mL), group B (0.83 mg/10 mL), group C (0.625 mg/10 mL), group D (0.455 mg/10 mL) and the control group without lead exposure for 18 hrs. The highest TAS was estimated in the control group without lead treatment in comparison with other groups (MAX, A, B, C, D). Statistical analyses showed significantly lower value (P 0.05) were detected in concentration of other studied parameters among observed groups, too.

  19. Biochemical parameters of bone metabolism in bone metastases of solid tumors (Review)

    NARCIS (Netherlands)

    Meijer, Wilhelmus; van der Veer, E; Willemse, P H

    1998-01-01

    The role of biochemical markers of bone metabolism in the diagnosis and monitoring of bone metastases in solid tumors is reviewed. Emphasis is on the recently developed markers, which may provide a more accurate quantitation of bone metabolism. In metastatic bone disease, bone formation and resorpti

  20. Hematological And Biochemical Parameters In Elite Soccer Players During A Competitive Half Season

    Directory of Open Access Journals (Sweden)

    Anđelković Marija

    2015-10-01

    Full Text Available Background: The purpose of the present study was to report and discuss the hematological and biochemical behavior of elite soccer players, in order to get more insight in the physiological characteristics of these sportsmen and to provide trainers and sports doctors with useful indicators.

  1. Influence of anti-epileptic drugs on hematological and biochemical parameters in patients with epilepsy

    Directory of Open Access Journals (Sweden)

    S. Dwajani

    2014-08-01

    Conclusions: The results of this study showed significant alterations in the levels of Hb and ALP with the use of AED polytherapy in PWE. Routine hematological and biochemical investigations may be considered during AED treatment in those patients receiving AED polytherapy. [Int J Basic Clin Pharmacol 2014; 3(4.000: 692-695

  2. Evaluation of biochemical, hematological and parasitological parameters of protein-deficient hamsters infected with Ancylostoma ceylanicum.

    Directory of Open Access Journals (Sweden)

    Carina P Pacanaro

    2014-09-01

    Full Text Available Hookworms infect millions of people worldwide and can cause severe clinical symptoms in their hosts. Prospective cohort studies in Brazil show high rates of hookworm reinfection in malnourished children compared to well-nourished children, despite previous treatment. Additionally, soil-transmitted helminth (STH infections can worsen the nutritional status of affected populations. Therefore, this study aims to clarify the effects of host malnutrition during Ancylostoma ceylanicum infection and how this infection affects host physiological parameters using a hamster model.Hamsters were divided into four experimental groups: normal diet or low-protein diet (also referred to as "malnourished" and A. ceylanicum infection or no infection. More severe pathogenesis was observed in the infected malnourished group, as demonstrated by significant decreases in the hemoglobin concentration, erythrocyte number and packed-cell volume compared to the non-infected malnourished group. Greater numbers of adult parasites and eggs were observed in the malnourished group compared to the control group; however, the oviposition rate was lower in the malnourished group. In general, greater values of total lipids were observed in malnourished animals compared to control animals, including lipids excreted in the stool.In this work, we have demonstrated that animals fed an isocaloric low-protein diet presented more severe pathogenesis when infected with A. ceylanicum. The increased lipid concentration in the liver and blood is related to the conversion of the excess carbohydrate into fatty acids that increase the concentration of triglycerides in general. Triglycerides were excreted in the feces, indicating that infection associated with malnutrition caused a greater loss of these molecules for this group of animals and confirming the hypothesis that both nutrition and infection are responsible for the malabsorption syndrome. Taken together, the results found in this work

  3. On-line estimation of concentration parameters in fermentation processes

    Institute of Scientific and Technical Information of China (English)

    XIONG Zhi-hua; HUANG Guo-hong; SHAO Hui-he

    2005-01-01

    It has long been thought that bioprocess, with their inherent measurement difficulties and complex dynamics, posed almost insurmountable problems to engineers. A novel software sensor is proposed to make more effective use of those measurements that are already available, which enable improvement in fermentation process control. The proposed method is based on mixtures of Gaussian processes (GP) with expectation maximization (EM) algorithm employed for parameter estimation of mixture of models. The mixture model can alleviate computational complexity of GP and also accord with changes of operating condition in fermentation processes, i.e., it would certainly be able to examine what types of process-knowledge would be most relevant for local models' specific operating points of the process and then combine them into a global one. Demonstrated by on-line estimate of yeast concentration in fermentation industry as an example, it is shown that soft sensor based state estimation is a powerful technique for both enhancing automatic control performance of biological systems and implementing on-line monitoring and optimization.

  4. Effects Of Music Therapy On Clinical And Biochemical Parameters Of Metabolic Syndrome

    Directory of Open Access Journals (Sweden)

    Rajnee

    2011-12-01

    Full Text Available Background: Music therapy is a new approach being used for the management of metabolic abnormalities and stress related illness.Objective: To study the effect of Music therapy on various clinical and biochemical parameters of Metabolic Syndrome.Methods: This cross sectional study was carried out on 100 patients of metabolic syndrome selected randomly. These patients were divided into two equal groups after age, sex adjustment. In control group (group I 50 patients underwent the conventional treatment. 50 patients in study group were treated with supervised music protocol along with conventional treatment. The Body Mass Index, ;Waist-Hip ratio, Blood pressure, Fasting blood sugar were monitored weekly while HbA1c and lipid profile were determined at the baseline and after three months of exposure to music therapy. Statistical analysis was performed by employing student t- test.Results: In the study group there was a significant decrease in BMI (27.18±5.02 to 25.44±3.49 kg/m2, p<0.05, waist hip ratio (0.95±0.05 to 0.93±0.05 cm, p<0.05, Fasting blood sugar (196.00±47.80mg/ dl to152.00±16.19mg/dl , p<0.001, HbA1c (8.41±1.31% to 7.08±0.78 % p<0.001, Systolic Blood Pressure (151.00±12.10 to 136±9.04 mmHg p<0.001, Diastolic Blood Pressure (94±4.80 to 86.44±3.16 mmHg, p<0.01, Mean serum cholesterol (257.80±18.92 to 229.12±17.82mg/dl, p<0.001 and triglycerides (180.86±14.04 to 136.50±8.92mg/dl, p<0.001, LDL (167.97±14.40 to 140.20±15.41mg/dl, p<0.001, and VLDL (33.60±2.88 to 28.04±3.08mg/dl, p<0.001 and increase in HDL (33.32±3.38 to 39.71±3.41mg/dl, p<0.001, when compared with those of control group not receiving the music therapy along with the conventional treatment.Conclusion: The promising outcomes of Music therapy showed that it may be considered as a useful adjunct to conventional treatment in management of the metabolic syndrome. This study advocates music therapy to establish it from a general well being concepts to a

  5. Features of the biochemical parameters of blood and exudates during tubercular exudative pleurisy

    Directory of Open Access Journals (Sweden)

    Ye. A. Yuriyeva

    2012-01-01

    Full Text Available Some biochemical indicators of peripheral blood and pleural fluid (level of the total protein, glucose, lactate dehydrogenase activity at patients with various variants of tubercular exudative pleurisy (MBT-positive and MBT-negative for the purpose of definition of possibility of use this indicators as criteria for differential diagnostics of a pleurisy, have been studied. It has been established that tubercular exudative pleurisy is characterized by the expressed changes of the biochemical status of blood and pleural fluid in comparison with that at healthy donors and patients with not tubercular exudative pleurisy, however, this changes in most cases are nonspecific and don't depend on a variant of tubercular exudative pleurisy.

  6. Evaluation of the effect of conventionally prepared swarna makshika bhasma on different bio-chemical parameters in experimental animals

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    Sudhaldev Mohapatra

    2011-01-01

    Full Text Available Swarna makshika (chalcopyrite bhasma (SMB has been used for different therapeutic purposes since long in Ayurveda. The present study is conducted to evaluate the effect of conventionally prepared SMB on different bio-chemical parameters in experimental animals, for providing scientific data base for its logical use in clinical practice. The genuine SMB was prepared by following classical techniques of shodhana and marana most commonly used by different Ayurvedic drug manufacturers. Shodhana was done by roasting raw swarna makshika with lemon juice for three days and marana was performed by 11 putas . The experimental animals (rats were divided into two groups. SMB mixed with diluted honey was administered orally in therapeutic dose to Group SMB and diluted honey only was administered to vehicle control Group, for 30 days. The blood samples were collected twice, after 15 days and after 30 days of drug administration and different biochemical investigations were done. Biochemical parameters were chosen based on references from Ayurvedic classics and contemporary medicine. It was observed that Hb% was found significantly increased and LDL and VLDL were found significantly decreased in Group SMB when compared with vehicle control group. This experimental data will help the clinician for the logical use of SMB in different disease conditions with findings like low Hb% and high LDL, VLDL levels.

  7. Spent Mushroom Substrate Influences Elk (Cervus Elaphus Canadensis) Hematological and Serum Biochemical Parameters

    OpenAIRE

    Park, Jae Hong; Kim, Sang Woo; DO, YOON JUNG; Kim, Hyun; Ko, Yeoung Gyu; Yang, Boh Suk; Shin, Daekeun; Cho, Young Moo

    2012-01-01

    The objective of this study was to evaluate the effect of spent mushroom substrate (SMS) derived from Pleurotus eryngii on the hematological and biochemical blood properties of elk. A total of 18, two and three-year-old elk were fed three different levels of SMS (0, 15 and 20%) in a corn-wheat bran diet for 80 days. The results indicated significantly high levels of blood monocytes, hemoglobin (Hb), and hematocrit (HCT) in elk fed 15% or 20% SMS (p

  8. Alteration of biochemical parameters after supplementation of multivitamins and minerals of sprayers on grape gardens of Western Maharashtra (India

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    Jyotsna A. Patil

    2014-01-01

    Full Text Available Background: In the horticulture sector, the land used for cash crop like grapes is increasing particularly in Maharashtra state. There is an increasing use of the pesticides in an attempt to increase the yield and reduce the post harvest losses. The environmental pollution and poisoning due to wide use of pesticide during grape cultivation may be much more important factors to disturb the socio economical status of uneducated farm worker in rural areas. Aim and Objectives: The study aimed to evaluate the pesticide exposure of the population associated with grape cultivation with respect to biochemical effects, oxidative stress, antioxidants status and protecting them from such effects by supplementing vitamins A to Z. Material and Methods: For this study 30 subjects with occupational pesticides exposure i.e. sprayers of grape gardens having age in the range of 20 to 45 years from Tasgaon taluka, Sangli district, (Western Maharashtra India were taken and all these study group subject,500 mg vitamin A to Z tablet / day for fifteen days were supplemented. The blood was collected by venipuncture from all these subjects for biochemical parameters assay before and after fifteen days of vitamins A to Z supplementation and all biochemical parameters were estimated by using standard methods. Results: Fifteen days vitamin A to Z and multi minerals supplementation to sprayers of grape gardens, we observed significant increase in serum Acetyl Cholinesterase (P<0.001, 2.32%, and decreased Aspartate Transaminase (P<0.05, 8.36%, Alanine Transaminase (P<0.05, 14.16%, serum Lipid Peroxide (P<0.001, 24.39%, Glutathione S-Transferase (P<0.01, 29.84% and increased RBC-Superoxide Dismutase (P<0.05, 11.55%, RBC-Catalase (P<0.001, 25.7%, serum zinc (P<0.01, 3.74%, copper (P<0.001, 4.13%, blood lead (P<0.01, 3.33% as compared to before vitamin A to Z and multi minerals supplementation and other biochemical parameters such as serum C-Reactive Proteins, Total Proteins

  9. Biochemical responses of filamentous algae in different aquatic ecosystems in South East Turkey and associated water quality parameters.

    Science.gov (United States)

    Çelekli, Abuzer; Arslanargun, Hamdullah; Soysal, Çiğdem; Gültekin, Emine; Bozkurt, Hüseyin

    2016-11-01

    To the best of our knowledge, any study about biochemical response of filamentous algae in the complex freshwater ecosystems has not been found in the literature. This study was designed to explore biochemical response of filamentous algae in different water bodies from May 2013 to October 2014, using multivariate approach in the South East of Turkey. Environmental variables were measured in situ: water temperature, oxygen concentration, saturation, conductivity, salinity, pH, redox potential, and total dissolved solid. Chemical variables of aqueous samples and biochemical compounds of filamentous algae were also measured. It was found that geographic position and anthropogenic activities had strong effect on physico-chemical variables of water bodies. Variation in environmental conditions caused change in algal biomass composition due to the different response of filamentous species, also indicated by FTIR analysis. Biochemical responses not only changed from species to species, but also varied for the same species at different sampling time and sampling stations. Multivariate analyses showed that heavy metals, nutrients, and water hardness were found as the important variables governing the temporal and spatial succession and biochemical compounds. Nutrients, especially nitrate, could stimulate pigment and total protein production, whereas high metal content had adverse effects. Amount of malondialdehyde (MDA), H2O2, total thiol groups, total phenolic compounds, proline, total carbohydrate, and metal bioaccumulation by filamentous algae could be closely related with heavy metals in the ecosystems. Significant increase in MDA, H2O2, total thiol group, total phenolic compounds, and proline productions by filamentous algae and chlorosis phenomenon seemed to be an important strategy for alleviating environmental factors-induced oxidative stress as biomarkers. PMID:27508982

  10. Seasonal Variations of some Hematological and Biochemical Parameters of the Carpathian Romanian Buffaloes. I. The Winter Period

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    Maria Serdaru

    2011-05-01

    Full Text Available Aim of this study was to investigate the blood parameters in Carpathian Romanian Buffaloes in order to establish their physiological ranges. Eight buffaloes from Şercaia-Braşov area were screened for hematological and biochemical profile during the winter period. Animals were fed on hay and beet pulp. Hematological parameters (hemoglobin, red blood cells, white blood cells, hematocrit, lymphocytes, monocytes, neutrophils and biochemical parameters (including total proteins, urea, cholesterol, alkaline phosphatase, aspartate transaminase, alanine transaminase, total calcium, inorganic phosphorus and magnesium were assessed. Preliminary results obtained after hematological examination were included within the following limits: 12,6-16,9 g/dL for hemoglobin; 6,48-9,51 x106/μL for red blood cells; 35,23-45,65% for hematocrit; 4,04-9,57 x103/μL for white blood cells; 45,3-76,8% for lymphocytes; 0,7-9,2% for monocytes; 22,5-45,9% for neutrophils. Results obtained after biochemical examination were included within the following limits: 4,0-9,2 g/dL for total proteins; 12-30 mg/dL for urea; 47-86 mg/dL for cholesterol; 92-171 U/L for alkaline phosphatase; 23-48 U/L for aspartate transaminase; 22-46 U/L for alanine transaminase; 5,0-9,3 mg/dL for total calcium; 1,3-3,9 mg/dL for inorganic phosphorus; 1,27-3.17 mg/dL for magnesium. Study will be continued during the grazing season in order to determine the seasonal variation of metabolic profile parameters.

  11. Response of thermochemical and biochemical conversion processes to lignin concentration in alfalfa stems

    Science.gov (United States)

    The technologies currently in place to convert lignocellulosic biomass to energy are either biochemical or thermochemical, the efficiencies of which may vary depending on the composition of the feedstock. One variable that conversion technologists have wrestled with, particularly in the simultaneous...

  12. THE EFFECT OF THE KETOGENIC DIET ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF THE CHILDREN WITH RESISTANT EPILEPSY

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    Mohammadreza ALAEI

    2010-04-01

    Full Text Available ObjectiveThe aim of this study was to evaluate the effect of the ketogenic diet on the growth parameters of the children with resistant epilepsy.Materials & MethodsA total of 36 children with resistant epilepsy who were 2 to 7 year old were put on the ketogenic diet. Their growth and biochemical parameters were studied at the beginning of the study and after 3 months.ResultsWeight decreased in all patients. Serum levels of hemoglobin, calcium, and blood sugar decreased significantly but remained in the normal range. Creatinine did not change, but BUN showed a significant increase.ConclusionWe can lower the complications of ketogenic diets by using more unsaturated fat, more water, and more minerals.Keywords: ketogenic diet, epilepsy, growth parameters, biochemicalparameters, children

  13. Effects of dietary administration of yarrow extract on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss

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    Mahmoud Nafisi Bahabadi

    2014-10-01

    Full Text Available The present study was conducted to investigate the clinical effects and possible side effects of yarrow extract (Achillea millefolium L. as feed additive on biochemical blood parameters and growth performance of rainbow trout (Oncorhynchus mykiss. Fishes were treated with 0 (control, 0.1, 0.5 and 1% of yarrow extract for 30 days. Plasma alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, alkaline phosphatase (ALP, creatine kinase (CK, peroxidase activity, total complement and lysozyme activity, glucose, total protein, triglyceride and cholesterol were measured after 15 and 30 days of yarrow treatment. There were no significant changes in the lysozyme activity and glucose levels. Total protein and globulin levels were significantly higher in the fish fed with diets enriched with 1% yarrow extract on day 30. Triglyceride and cholesterol levels was significantly decreased in the fish fed with diets containing 0.5% and 1% yarrow extract on day 30 (P<0.05. LDH, CK and peroxidase activities in the fish fed with diets having 1% yarrow extract were significantly decreased at the end of the experiment (P<0.05. In contrast, a significant increase in AST, ALP and total complement activity was observed in the fish fed with 1% yarrow extract diet, on day 15 (P<0.05. The weight gain and specific growth rate increased and food conversion ratio decreased in in the fish fed 1% yarrow extract on day 30. Condition factor in the fish fed with yarrow extract was significantly higher than control group on 30 day. In conclusion, on the basis of these results, oral administration of yarrow extract up to 0.5% have not side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 1% of yarrow extract caused cytotoxicity and modifications in blood biochemical parameters of fish.

  14. Correlation between Aminotransferase Ratio (AST/ALT and Other Biochemical Parameters in Chronic Liver Disease of Viral Origin

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    Shah Md Fazlul Karim

    2015-03-01

    Full Text Available Background: In recent years the ratio of aspartate aminotransferase (AST to alanine aminotransferase (ALT in patients of chronic liver disease (CLD of various origins has gained much attention. This variable is readily available, easy to interpret, and inexpensive and the clinical utility of the AST/ALT ratio in the diagnostic workup of patients with CLD is quite promising. Objective: The present study was designed to find out the link between aminotransferase (AST/ALT ratio with commonly measured biochemical parameters of liver function tests in CLD of viral origin. Materials and method: This cross sectional study was carried out in the department of Biochemistry, Sir Salimullah Medical College, Dhaka, Bangladesh. Forty four biopsy proven diagnosed subjects of chronic viral hepatitis without cirrhosis of both sex were selected purposively. With aseptic precaution 5 mL venous blood was collected from each subject and common liver function tests (serum AST, ALT, AST/ALT ratio, alkaline phosphatase, total bilirubin, serum total protein, serum albumin, serum globulin, serum albumin/globulin ratio, prothrombin time and viral serology (HBsAg, Anti HDV antibody, Anti HCV antibody were performed. Data were analyzed by SPSS version 19 for Windows. Pearson’s correlation test was done to determine association between AST/ALT with other biochemical parameters. Results: Mean(±SD age of the study subjects was 32.55±10.55 years (range 20-50 years with 48 (77.7% male and 14 (22.6% female subjects. Pearson’s correlation test was done between AST to ALT ratio with other biochemical parameters and prothrombin time showed significant positive correlation (p <0.01. Conclusion: In our study we found significant positive correlation between AST/ALT with prothrombin time in CLD subjects without cirrhosis.

  15. Effect of imatinib on the biochemical parameters of the reproductive function in male Swiss albino mice

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    A M Prasad

    2011-01-01

    Full Text Available Background: Treatment of cancers with cytotoxic agents such as tyrosine kinase inhibiting drugs often, but not always, result in transient to permanent testicular dysfunction. Germ cells are important targets of many chemicals. Most of the drugs are genotoxins and induce irreversible effect on genetic makeup. These mutagenic changes are proportionally related to carcinogenesis. This is alarmingly dangerous in youth and children, since these effects last longer, affecting fertility or forming basis for carcinogenesis. There is paucity of reports on planned studies of imatinib on the testicular function. Hence, the study was planned to assess the effects of imatinib on biochemical markers of testicular functions in male Swiss albino mice. Materials and Methods: Male Swiss albino mice were treated with imatinib and sacrificed at the end of first, second, fourth, fifth, seventh, and tenth week after the last exposure to imatinib. The testis were removed, weighed, and processed for biochemical analysis. Results: The intratesticular testosterone level was significantly (P<0.001 reduced in treated groups and severe effect was observed on week 4 and 5. The intratesticular lactate dehydrogenase (LDH level was significantly increased by imatinib in all treated groups up to week 5. Conclusion: Imatinib does affect testosterone and LDH level significantly, but this effect is reversible once the drug is withdrawn. This finding may help the clinicians to plan and address the fertility-related issues in young patients of reproductive age who are being treated with imatinib for gastrointestinal tumors and chronic myeloid leukemia.

  16. THE VARIATION OF SOME BIOCHEMICAL PARAMETERS FROM FRESH COW CHEESE CREAM TYPE WITH ADDED AROMATIC PLANTS

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    CRISTIANA DIACONESCU

    2013-12-01

    Full Text Available Recent advanced studies in the field of cheese fabrication technology include, amongother, the usage of aromatic plants and spices as a mean of improving theorganoleptic properties and as a mean of decreasing the lipidic peroxidationphenomenon. For this purpose, three types of fresh cow cheese produced by S.C.Marion Invest Trade 94 SRL, experimentally added with dill, savory and rosemary,were comparatively studied from a chemical point of view (pH, acidity, humidity, ashand a biochemical point of view(raw fat, total proteins, total cholesterol, acidphosphatase’s enzymatic activity, superoxide dismutase’s enzymatic activity, calciumcontent.From a chemical point of view, no significant differences appeared between the creamcheese types analyzed, excepting the pH( lower in the cheeses with dill and rosemaryFrom a biochemical point of view, two phenomenon can be observed: a significantdecrease of the acidic phosphatase activity in the cheeses with added dill androsemary ( positively correlating with the pH value and decreased values of the SODactivity in all cheese types.

  17. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase

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    Flisser Ana

    2011-05-01

    Full Text Available Abstract Background Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP and the active labor phase (ActLP. Methods Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Results Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Conclusion Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  18. Changes in quality and biochemical parameters in 'Idared' apples during prolonged shelf life and 1-MCP treatment.

    Science.gov (United States)

    Bizjak, Jan; Slatnar, Ana; Stampar, Franci; Veberic, Robert

    2012-12-01

    In this study, changes in quality and various biochemical parameters of 'Idared' apples during prolonged shelf life period after ultra-low oxygen (ULO) storage were investigated. Additionally, the impact of the postharvest application of 1-methylcyclopropene (1-MCP) on different parameters was evaluated. After the harvest, apples were stored in the ULO storage for 6 months and then exposed to room temperature. Fruit firmness, peel color, and changes in sugars, organic acids and phenolics were monitored during the 3 weeks of shelf life. Malic acid, sugars and firmness decreased at room temperature. However, the color of the apples remained unchanged. The level of citric and ascorbic acid remained constant. Levels of phenolics in the peel increased significantly, whereas remained constant in the pulp of apples. 1-MCP treatment resulted in higher amounts of fructose and glucose, malic acid and greater firmness of apples. However, 1-MCP did not influence the phenolic content, ascorbic acid or color. The results obtained indicate that the content of different health-promoting compounds of apples does not change dramatically at room temperature. At the same time these results suggest that 1-MCP could be useful for maintaining certain quality and biochemical parameters and might extend the shelf life of apples.

  19. INVESTIGATION OF EFFECT OF MURRAYA KOENIGII ON BIOPHYSICAL AND BIOCHEMICAL PARAMETERS OF WOUND IN DIABETIC HYPERLIPIDEMIC WISTAR RATS

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    Vikram Kumar*, Angshu Bandyopadhyay and Vikram Sharma

    2012-06-01

    Full Text Available Plants have been used in traditional medicine for several thousand years. India is perhaps the largest producer of medicinal herbs and is rightly called the “Botanical garden of the World”. Murraya koenigii Linn. commonly known as Meethi neem, belongs to the family Rutaceae. The curry tree is native to India and it is found almost everywhere in the Indian subcontinent excluding the higher levels of Himalayas. The effect of Murraya koenigii leaves aqueous extract on biophysical and biochemical parameters of wound were studied by dead space wound model in diabetic hyperlipidemic rats. In dead space wound model, animals treated with Murraya koenigii (oral administration of variable dosage level 200mg/kg, 300mg/kg and 400mg/kg leaves aqueous extract showed significant increase in Wet & Dry granulations tissue weight (biophysical parameter and hydroxyprolin content (biochemical parameter when compared to the diabetic hyperlipidemic control group rats. In this study, very significant (p<0.001 result was found with 300mg/kg dose level because the effect was dose dependent up to 300mg equivalent of extract. The results suggested that aqueous extract of Murraya koenigii possess significant wound healing potential in diabetic hyperlipidemic rats. Further studies may reveal the exact mechanisms of action responsible for the wound healing activity of Murraya koenigii leaves aqueous extract in diabetic hyperlipidemic condition.

  20. Reference values for selected hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats

    OpenAIRE

    ŞİMŞEK, Özkan; ARIKAN, ŞEVKET; ÇINAR, Miyase

    2015-01-01

    The aim of this study was to monitor some hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats. In the present study, 35 blood samples obtained before and during pregnancy from 7 adult cats were used. Blood was collected from cats before pregnancy and on days 15, 30, 45, and 55 of gestation. The blood samples were analyzed in terms of WBC, RBC, Hb, PCV, MCV, MCH, MCHC, GRA, MON, and LYM values. Additionally, serum ALT, AST, ALP, GGT, LDH, and C...

  1. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    Science.gov (United States)

    Zhou, Zhenming; Zhou, Bo; Ren, Liping; Meng, Qingxiang

    2014-01-01

    Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg) were used to compare a standard total mix ration (TMR) with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML) or sun-dried mulberry fruit pomace (SMFP). Experimental diets had similar amounts of crude protein (CP), acid detergent fiber (ADF), and metabolizable energy (ME). Animals were divided into three groups: control group (CONT), 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG), and dry matter intake (DMI), was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks). There were no differences in final body weight (P = 0.743), ADG (P = 0.425), DMI (P = 0.642), or ADG/DMI (P = 0.236) between the groups. There were no differences (P = 0.2024) in rumen pH values; ammonia N was lower (P = 0.0076) in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098). No differences were observed in carcass characteristics (P ≥ 0.513), tenderness (P = 0.844), adipose and lean color values (P ≥ 0.149), and chemical composition (P ≥ 0.400); however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034). In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content. PMID:24427304

  2. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    Directory of Open Access Journals (Sweden)

    Zhenming Zhou

    Full Text Available Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg were used to compare a standard total mix ration (TMR with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML or sun-dried mulberry fruit pomace (SMFP. Experimental diets had similar amounts of crude protein (CP, acid detergent fiber (ADF, and metabolizable energy (ME. Animals were divided into three groups: control group (CONT, 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG, and dry matter intake (DMI, was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks. There were no differences in final body weight (P = 0.743, ADG (P = 0.425, DMI (P = 0.642, or ADG/DMI (P = 0.236 between the groups. There were no differences (P = 0.2024 in rumen pH values; ammonia N was lower (P = 0.0076 in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098. No differences were observed in carcass characteristics (P ≥ 0.513, tenderness (P = 0.844, adipose and lean color values (P ≥ 0.149, and chemical composition (P ≥ 0.400; however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034. In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content.

  3. Influence of drug concentration on the diffusion parameters of caffeine

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    R Ben Mustapha

    2011-01-01

    Full Text Available Background and Objectives : In the fields of the pharmaceutical and cosmetic industries and in toxicology, the study of the skin penetration of molecules is very interesting. Various studies have considered the impact of different physicochemical drug characteristics, skin thickness, and formulations, on the transition from the surface of the skin to the underlying tissues or to the systemic circulation; however, the influence of drug concentration on the permeation flux of molecules has rarely been raised. Our study aims to discover the influence of caffeine concentration in a formulation on the percutaneous penetration from gels, as a result of different dose applications to polysulfate membrane and human skin. Materials and Methods : For this purpose, three identical base gels were used at 1, 3, and 5% of caffeine, to evaluate the effect of the concentration of caffeine on in vitro release through the synthetic membrane and ex vivo permeation through the human skin, using diffusion Franz TM cells. Results : The diffusion through the epidermal tissue was significantly slower than through the synthetic membrane, which recorded an increase of flux with an increase in the concentration of caffeine. The skin permeation study showed that diffusion depended not only on the concentration, but also on the deposited amount of gel. Nevertheless, for the same amount of caffeine applied, the flux was more significant from the less concentrated gel. Conclusion : Among all the different concentrations of caffeine examined, 1% gel of caffeine applied at 5 mg / cm 2 showed the highest absorption characteristics across human skin.

  4. Assessment of Some Biochemical Parameters for Early Detection of Protein Energy Malnutrition

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    Mohsen M. Deeb, Mostafa M. EShakankery* ,Nagwa Mohamed

    2005-12-01

    Full Text Available Protein energy malnutrition (PEM is a common health problem in developing countries. The mild form of PEM results in growth retardation while severe forms result in the syndromes of marasmus and kwashiorkor. Mild PEM was described as a condition in which specific or , non specific clinical signs , as well as non specific laboratory indices are present without any of the classical manifestations of nutritional deficiency. Objective of this work was to assess serum transferrin and fibronectin as a biochemical markers for early detection of PEM. We studied 42 children: 30 patients had PEM (15 cases were mild to moderate PEM and 15 cases were severe PEM with their age ranged between 4-36 months and the remaining 12 children were age and sex matched healthy children as controls. All studied children were subjected to: full history taking, thorough clinical examination including anthropometric measurements [weight, hight,body mass,index (BMI, and mid arm circumference (MAC] as well as estimation of serum albumin, serum transferrin, fibronectin, Hb and urine analysis. Results revealed significant reduction in all anthropometric measurements in both mild to moderate and severe PEM patients as compared to control group . Serum albumin was significantly lowered in severe PEM as compared to control and still within normal level in mild to moderate PEM patients. Serum transferrin and fibronectin were significantly lowered in mild to moderate PEM as compared to controls and more reduction in severe form of PEM as compared to controls . Serum fibronectin and transferrin correlated positively with BMI (P <0.05 and serum albumin (P <0.001 in both mild to moderate and severe PEM . In conclusion serum fibronectin and transferrin are markedly reduced in patients with mild to moderate PEM and correlated positively with BMI and serum albumin. This represents an evidence for the role of serum fibronectin and transferrin as biochemical markers for early detection of

  5. Inversion of Biochemical Parameters by Selection of Proper Vegetation Index in Winter Wheat

    Institute of Scientific and Technical Information of China (English)

    HUANG Wen-jiang; WANG Ji-hua; LIU Liang-yun; ZHAO Chun-jiang; WANG Zhi-jie; WANG Jin-di

    2004-01-01

    Recent studies have demonstrated the application of vegetation indices from canopy reflected spectrum for inversion of chlorophyll concentration.Some indices are both response to variations of vegetation and environmental factors.Canopy chlorophyll concentration,an indicator of photosynthesis activity,is related to nitrogen concentration in green vegetation and serves as an indicator of the crop response to soil nitrogen fertilizer application.The combination of normalized difference vegetation index (NDVI) and photochemical reflectance index (PRI) can reduce the effect of leaf area index (LAI) and soil background.The canopy chlorophyll inversion index (CCII) was proved to be sensitive to chlorophyll concentration and very resistant to the other variations.This paper introduced the ratio of TCARI/OSAVI to make accurate predictions of winter wheat chlorophyll concentration under different cultivars.It indicated that canopy chlorophyll concentration could be evaluated by some combined vegetation indices.

  6. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

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    Ye. Barylo

    2016-06-01

    Full Text Available Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae of brown trout, rainbow trout and brook trout we used as study materials. Morphometric parameters we studied by the methods of N. O. Lange, E. N. Dmitrieva. The content of total lipids was determined in accordance with Folch. in the tissuesm, which were taken for biochemical studies. Separate classes of lipids were received by thin layer chromatography. Findings. We carried out a comparative analysis of morphometric measurements and biochemical parameters of one-day pre-larval brown trout, rainbow trout and brook trout based on the obtained data. We investigated morphometric and biochemical specific features of pre-larvae in post-embryonic period and showed the species differences of morphometric measurements. Significant differences were observed between the content of lipids in the body and yolk sac of free embryos. In particular, a higher content of phospholipids and triglycerides was observed in the body of brook trout compared to brown trout. We also recorded higher contents of mono- and diacylglycerols, free cholesterol, non-etherified fatty acids (NEFA, triacylglycerols and cholesterol esters in the yolk sac of brook trout. Compared to brown trout, rainbow trout had a significant increase in mono- and diacylglycerols, free cholesterol and NEFA in both body and yolk sac as well higher levels of total lipids, triacylglycerols and cholesterol esters were registered in yolk sac. Originality. For the first time we carried out and compared the specific features of pre-larval brown trout, rainbow trout and brook trout in the

  7. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

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    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination. The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth condition of the respective colony. All the samples coming from sick colonieswere removed, only healthy adult honeybees coming from strong colonies were keptand used for hemolymph collection. By special methods, samples of hemolymph (300μl/sample collected from about 50 individuals, were analyzed both during the activeseason (spring/summer and the inactive season (autumn/winter. The study wascarried out on 50 samples of undiluted hemolymph taken from a total number of about2,500 honeybees. The following 21 biochemical parameters were analyzed: GLU(mg/dl, HDL-c (mg/dl, ALP (UI/l, T-cho (mg/dl, Tprot (mg/dl, Alb. (g/dl, BUN(mg/dl, LDH (UI/l, CPK (UI/l, Mg (mg/dl, FRA (μm/l, IP (mg/dl, GGT (UI/l,GOT (UI/l, GPT (UI/l, Ca (mg/dl, Cre (mg/dl, Amy (UI/l, T–BIL (mg/dl, TG(mg/dl, UA (mg/dl. The test was carried out after the collection and processing of thesamples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique,respectively.

  8. Effects of Pre-induction Administration of Allium Sativum on Some Biochemical Parameters in Alloxan Induced Diabetic Rats

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    Ojo Rotimi Johnson

    2012-12-01

    Full Text Available The aim of the present study was to investigate the effects of pre- and post- administration of garlic extract on serum glucose and some biochemical parameters in alloxan-induced diabetic rats to show the preventive and ameliorating effects in alloxan induced-diabetic rats. Rats were divided into 4 groups; normal control rats, diabetic control rats, diabetic rats post-treated with garlic extract and rats pre-treated with garlic extract before induction. Garlic extract was administered orally for 2 weeks to post-treated rats and 3 weeks to pre-treated rats and they were compared with the normal and diabetic groups, respectively. Serum glucose was reduced significantly in both post-treated and pre-treated groups. The post-treatment with garlic extract reduced serum cholesterol, but pre-treatment with garlic extract produced significant change compare to the diabetic control. The serum creatinine and urea levels were significantly reduced in post-treated group and pretreated group compare to the diabetic control group. It is concluded that the consumption of garlic produced a significant hypoglycaemic and hypolipidemic effects in diabetic rats and also, garlic is capable of protecting the liver and the kidney functions in alloxan-induced diabetic rats as shown in the activities of serum enzymes and other biochemical parameters examined.

  9. Therapeutic competence of dried garlic powder (Allium sativum on biochemical parameters in lead (Pb exposed broiler chickens

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    Md. Anwar Hossain

    2014-12-01

    Full Text Available The study was conducted to assess the therapeutic competence of garlic (Allium sativum in lead (Pb exposed chickens. The experimental birds (n=350 were grouped into T0 (as control, T1, T2, T3 and T4. The birds of group T1 was provided with lead acetate at 100 mg/kg body weight. Group T2 had lead acetate at 100 mg/kg b.wt. + 1% garlic supplement, whereas group T3 was fed with lead acetate at 100 mg/kg b.wt. + 2% garlic supplement, and group T4 had lead acetate at 100 mg/kg b.wt. + 4% garlic supplement. The mean values (mg/dL of uric acid, blood urea nitrogen, creatinine, cholesterol, triglycerides, low density lipoprotein, high density lipoprotein, and blood glucose in the birds of group T1 were significantly increased (p<0.01 on day 42 of post-treatment. Elevation of these parameters was suggestive for the pathological involvement of different organs like liver, kidney, muscles. Statistical analysis of variance indicated that lead acetate at 100 mg/kg b.wt. + 2% garlic supplement (T3 resulted significant (p<0.01 ameliorative effect on the biochemical parameters as compared to the group T2 and T4. In conclusion, potency of garlic in reversion of the values of the biochemical properties in Pb exposed chickens was close to the normal levels of the values.

  10. THE EFFECT OF THE KETOGENIC DIET ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF THE CHILDREN WITH RESISTANT EPILEPSY

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    Mohammadreza ALAEI

    2010-03-01

    Full Text Available ObjectiveThe aim of this study was to evaluate the effect of the ketogenic diet on the growth parameters of the children with resistant epilepsy.Materials & MethodsA total of 36 children with resistant epilepsy who were 2 to 7 year old were put on the ketogenic diet. Their growth and biochemical parameters were studied at the beginning of the study and after 3 months.ResultsWeight decreased in all patients. Serum levels of hemoglobin, calcium, and blood sugar decreased significantly but remained in the normal range. Creatinine did not change, but BUN showed a significant increase.ConclusionWe can lower the complications of ketogenic diets by using more unsaturated fat, more water, and more minerals.

  11. STUDY OF BIOCHEMICAL PARAMETERS IN AMNIOTIC FLUID FOR ASSESSMENT OF FOETAL MATURITY IN CASES OF NORMAL PREGNANCY

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    Leela

    2015-11-01

    Full Text Available Assessment of foetal maturity had been proven of value in evaluating the foetal condition. Accurate assessment of foetal maturity is essential for the proper timing of delivery in various risk pregnancies. Amniotic Fluid analysis for foetal maturity had been of proven value. In the present study, study of biochemical parameters in amniotic fluid in respect of Creatinine, Uric Acid, Urea, Total Proteins, and Electrolytes i.e. Sodium, Potassium and Chloride has been done, along with Serum Electrolytes. Standard methodologies were adopted. The observations in the present study correlated with the works of Chadick et al and Pitkin and Zwirek. The levels of Creatinine, Uric Acid and Urea in Amniotic Fluid showed elevation, while Total Proteins and Serum Sodium showed a decline, as gestation progressed. The Serum and Amniotic Fluid Potassium and Chloride levels remain almost constant throughout the pregnancy. Thus, it is observed that the use of multiple parameters is desirable for accurate assessment of foetal maturity.

  12. Early detection of crop injury from herbicide glyphosate by leaf biochemical parameter inversion

    Science.gov (United States)

    Zhao, Feng; Guo, Yiqing; Huang, Yanbo; Reddy, Krishna N.; Lee, Matthew A.; Fletcher, Reginald S.; Thomson, Steven J.

    2014-09-01

    Early detection of crop injury from herbicide glyphosate is of significant importance in crop management. In this paper, we attempt to detect glyphosate-induced crop injury by PROSPECT (leaf optical PROperty SPECTra model) inversion through leaf hyperspectral reflectance measurements for non-Glyphosate-Resistant (non-GR) soybean and non-GR cotton leaves. The PROSPECT model was inverted to retrieve chlorophyll content (Ca+b), equivalent water thickness (Cw), and leaf mass per area (Cm) from leaf hyperspectral reflectance spectra. The leaf stress conditions were then evaluated by examining the temporal variations of these biochemical constituents after glyphosate treatment. The approach was validated with greenhouse-measured datasets. Results indicated that the leaf injury caused by glyphosate treatments could be detected shortly after the spraying for both soybean and cotton by PROSPECT inversion, with Ca+b of the leaves treated with high dose solution decreasing more rapidly compared with leaves left untreated, whereas the Cw and Cm showed no obvious difference between treated and untreated leaves. For both non-GR soybean and non-GR cotton, the retrieved Ca+b values of the glyphosate treated plants from leaf hyperspectral data could be distinguished from that of the untreated plants within 48 h after the treatment, which could be employed as a useful indicator for glyphosate injury detection. These findings demonstrate the feasibility of applying the PROSPECT inversion technique for the early detection of leaf injury from glyphosate and its potential for agricultural plant status monitoring.

  13. Effect of gamma irradiation on seed germination, growth and biochemical parameters of Terminalia arjuna ROXB

    International Nuclear Information System (INIS)

    Terminalia arjuna, a potent medicinal plant for the treatment of cardiac disease was chosen for the study. About 300 seeds of T. arjuna were irradiated with different doses (0, 10, 25, 50, 100, 150 and 200 Gy) of gamma radiation using 60Co gamma source. Effect of gamma radiation on growth and biochemical constituents were compared with the control of plants. Germination speed at 25 Gy was found to be 0.65 which was double the control plants. An increase in germination percentage, vigour index and relative growth rate in terms of dry weight was noticed at lower dosages. There was a gradual increase in shoot and root length at 25 and 50 Gy which decreased at higher doses of radiation. Proline content increased with increasing doses of radiation. Chlorophyll content was found to be 12.2 mg/gfw at 100 Gy while in control it was 8.44 mg/gfw. Increased phenolic content and radical scavenging capacity was observed at 25 Gy and 150 Gy. Hence, lower doses of radiation treatment to seeds may be recommended to increase the germination, growth and to increase secondary plant products. (author)

  14. Effect of gamma irradiation on seed germination, growth and biochemical parameters of Flacourtia Montana Graham

    International Nuclear Information System (INIS)

    Flacourtia montana an endemic species of Western Ghat which is well known for its ethno-medicinal value was chosen for the study. Matured seeds collected from Beithangady region of Dakshina Kannada district were irradiated with different doses (0, 25, 50, 100, 150, 200, 250, 300 Gy) of gamma radiation using 60Co gamma source at Board of Radiation and Isotope Technology (BRIT). Effect of gamma radiation on growth and biochemical constituents were compared with that of control plants. An increase in germination percentage, speed and vigour index was observed at lower dosages, and the dose of 100 Gy was found to be more effective out of the selected doses as far as growth is concerned. Doses of 25 and 100 Gy showed 95 and 100% germination respectively but the germination was reduced at higher dosages. Total chlorophyll content at 10 Gy was found to be 19.56 mg/gfw while in control was 14 mg/gfw. Phenolic content was higher at 50 Gy and proline content at 150 Gy. Slight increase in carbohydrate content was observed in irradiated samples. Hence, lower doses of radiation treatment may be recommended to increase growth and phenolic content of plant F.montana. (author)

  15. Correlation between ovarian morphology and biochemical and hormonal parameters in polycystic ovary syndrome

    Science.gov (United States)

    Inan, Cihan; Karadag, Cihan

    2016-01-01

    Objective: To determine the biochemical and hormonal differences in polycystic ovary syndrome (PCOS) patients with and without polycystic ovary (PCO) morphology and to evaluate the outcomes resulting from those differences. Methods: The study included a total of 83 patients with PCOS; 43 of them had PCO morphology (Group-I) and 40 did not (Group-II). Serum LDL, HDL, total cholesterol, triglyceride (TG), total testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), 17b-estradiol (E2), prolactin (PRL), thyroid stimulating hormone (TSH), sex hormone binding globulin (SHBG), glucose and insulin levels were determined. Homoeostatic model assessment insulin resistance (HOMA-IR) index was calculated. Results: The two groups were similar with respect to BMI. The systolic and diastolic blood pressure measurements of Group-I were significantly lower (p<0.01). Serum mean level of LH (p=0.026) and the mean LH/FSH (p=0.001) level of Group-I were significantly higher than Group-II. The total cholesterol and triglyceride levels of Group-I were significantly lower (p<0.05, p<0.01). The mean HOMA-IR level of Group-I was significantly lower than Group-II (p=0.004). Conclusions: The group without PCO morphology had a higher risk than the other group in terms of increased insulin resistance, dyslipidemia and cardiovascular diseases due to effects of hyperandrogenism. PMID:27375725

  16. [Effects of drought stress on physiological and biochemical parameters of Dahlia pinnata].

    Science.gov (United States)

    Fan, Su-lu; Yuan, Zhao-he; Feng, Li-juan; Wang, Xiao-hui; Ding, Xue-mei; Zhen, Hong-li

    2011-03-01

    Taking Dahlia pinnata 'Fenxishi' as test material, this paper studied its leaf physiological and biochemical responses to different degrees of drought stress and re-watering. With the increasing extent and duration of drought stress, the leaf relative water content, water potential, and chlorophyll content of D. pinnata 'Fenxishi' decreased significantly, leaf relative electric conductivity and malondialdehyde (MDA) content had a significant increase, plasma membrane was damaged, and massive ions were leaked out. The damage of plasma membrane was most serious under severe stress, and could not recover to the control level after re-watering. The leaf soluble sugar and proline contents also increased significantly with increasing extent and duration of drought stress. Especially for proline content, it was increased significantly in the later period of moderate and severe stresses, suggesting its lower sensitivity to water deficit. The leaf soluble protein content had a trend of down-up-down, while the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) decreased after an initial increase. There were some differences in the responses of the three enzymes to drought stress and reactive oxygen, exhibiting their coordinating role. PMID:21657020

  17. Biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) exposed to in vivo sub-lethal copper concentrations

    Science.gov (United States)

    Li, Yifeng; Gu, Zhongqi; Liu, Hong; Shen, Heding; Yang, Jinglong

    2012-09-01

    Many aquatic organisms are negatively affected by exposure to high copper concentrations. We investigated the biochemical response of the mussel Mytilus coruscus (Mytiloida: Mytilidae) to copper exposure. In vivo bioassays using M. coruscus and different copper concentrations were conducted. The activity of six biomarkers, namely superoxide dismutase (SOD), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were measured. Survival rates decreased with increased copper concentrations and exposure times. The LC50 values at 48, 72, and 96 h exposure were 0.48, 0.37, and 0.32 mg/L, respectively. Within digestive glands, CAT activity increased with increasing Cu concentrations. The activity of AKP showed no significant change, while the remaining four enzymes showed decreasing activity with increasing Cu concentrations. Within the gills, AKP activity increased when the Cu concentration was 0.05 mg/L, but showed no significant changes at higher concentrations. Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration. The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L. GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L. Within the adductor muscle, AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations. ACP activity within adductor muscle tissue showed no change, while activities of CAT, GOT and GPT decreased with increasing Cu concentrations. SOD activity within the adductor muscle tissue significantly decreased at the 0.02, 0.05 and 0.2 mg/L exposure concentrations. Our results show tissue specific differences for the six biomarkers in for M. coruscus. Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated, and are essential preliminary steps in development

  18. Evaluation of Some Biochemical Parameters and Brain Oxidative Stress in Experimental Rats Exposed Chronically to Silver Nitrate and the Protective Role of Vitamin E and Selenium

    Science.gov (United States)

    Gueroui, Mouna; Kechrid, Zine

    2016-01-01

    Due to undesirable hazardous interactions with biological systems, this investigation was undertaken to evaluate the effect of chronic exposure to silver on certain biochemical and some oxidative stress parameters with histopathological examination of brain, as well as the possible protective role of selenium and/or vitamin E as nutritional supplements. Thirty six male rats were divided into six groups of six each: the first group used as a control group. Group II given both vitamin E (400 mg/kg) of diet and selenium (Se) (1 mg/L) in their drinking water. Group III given silver as silver nitrate (AgNO3) (20 mg/L). Group IV given vitamin E and AgNO3. Group V given both AgNO3 and selenium. Group VI given AgNO3, vitamin E and Se. The animals were in the same exposure conditions for 3 months. According to the results which have been obtained; there was an increase in serum lactate dehydrogenase (LDH), lipase activities and cholesterol level, a decrease in serum total protein, calcium and alkaline phosphatase (ALP) activity in Ag-intoxicated rats. Moreover, the findings showed that Ag+ ions affected antioxidant defense system by decreasing superoxide dismutase (SOD) activity and increasing vitamin E concentration with a high level of malondialdehyde (MDA) in brain tissue. The histological examination also exhibited some nervous tissue alterations including hemorrhage and cytoplasm vacuolization. However, the co-administration of selenium and/or vitamin E ameliorated the biochemical parameters and restored the histological alterations. In conclusion, this study indicated that silver could cause harmful effects in animal body and these effects can be more toxic in high concentrations or prolonged time exposure to this metal. However, selenium and vitamin E act as powerful antioxidants which may exercise adverse effect against the toxicity of this metal.

  19. [Serum leptin concentration and some lipid parameters in vegetarian children].

    Science.gov (United States)

    Laskowska-Klita, Teresa; Ambroszkiewicz, Jadwiga; Klemarczyk, Witold

    2004-04-01

    Leptin, a hormone from adipose tissue, regulates feeding behavior, satiation rate, energy expenditure and also plays an important role in maturation and reproduction. Recent studies support the concept that several factors such as a diet may influence on leptin levels. The aim of this study was to investigate serum concentration of leptin and lipids status in prepubertal children aged from 2 to 10 years with two different nutritional habits: vegetarian (n = 24) and omnivorous diet (n = 20). Serum leptin concentration was determined by immunoenzymeassay (ELISA). Serum lipids (cholesterol, HDL- and LDL-cholesterol, triglyceride) were measured by enzymatic and apolipoproteins by immunoturbidimetric methods. We noticed that in vegetarian diet there is a high rate of fiber (nearly twice as high as in omnivorous diet) and polyunsaturated acids (35% as much). In our study vegetarian children had lower total cholesterol and cholesterol in fractions HDL and LDL than meat eaters did. Also the apolipoproteins levels in vegetarian children were significantly below that of nonvegetarians. There is no differences in triglyceride concentration between the two groups of children. The mean serum leptin level in vegetarian children was significantly lower (3.1 +/- 1.2 ng/mL) as compared with the omnivores (5.6 +/- 2.1 ng/mL) (p < 0.0001).

  20. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    Energy Technology Data Exchange (ETDEWEB)

    Raposo, F.; Borja, R.; Rincon, B. [Instituto de la Grasa (CSIC), Avda. Padre Garcia Tejero 4, 41012 Seville (Spain); Jimenez, A.M. [Departamento de Ciencias Ambientales, Universidad Pablo de Olavide, Ctra. De Utrera, km 1, 41013 Sevilla (Spain)

    2008-12-15

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (Y{sub M,ult}) decreased considerably from 227{+-}23 to 107{+-}11 ml CH{sub 4} at standard temperature and pressure (STP) conditions g{sup -1} VS{sub added} when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g{sup -1} VS{sub added} was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates. (author)

  1. Effect of maternal exercise on biochemical parameters in rats submitted to neonatal hypoxia-ischemia.

    Science.gov (United States)

    Marcelino, Thiago Beltram; de Lemos Rodrigues, Patrícia Idalina; Miguel, Patrícia Maidana; Netto, Carlos Alexandre; Pereira Silva, Lenir Orlandi; Matté, Cristiane

    2015-10-01

    Pregnancy is a critical period for brain metabolic programming, being affected by individual environment, such as nutrition, stress, and physical exercise. In this context, we previously reported a cerebral antioxidant upregulation and mitochondrial biogenesis in the offspring delivered from exercised mothers, which could provide neuroprotection against neonatal insults. Hypoxia-ischemia (HI) encephalopathy is one of the most studied models of neonatal brain injury; disrupting motor, cognitive, and learning abilities. Physiopathology includes oxidative stress, allied to mitochondria energy production failure, glutamatergic excitotoxicity, and cell death. In this study we evaluated the effect of maternal swimming during pregnancy on offspring׳s brain oxidative status evaluated fourteen days after HI stablishment. Swimming exercise was performed by female adult rats one week before and during pregnancy, in controlled environment. Their offspring was submitted to HI on postnatal day 7, and the brain samples for biochemical assays were obtained in the weaning. Contrary to our expectations, maternal exercise did not prevent the oxidative alterations observed in brain from HI-rats. In a general way, we found a positive modulation in the activities of antioxidant enzymes, measured two weeks after HI, in hippocampus, striatum, and cerebellum of pups delivered from exercised mothers. Reactive species levels were modulated differently in each structure evaluated. Considering the scenery presented, we concluded that HI elicited a neurometabolic adaptation in both brain hemispheres, particularly in hippocampus, parietal cortex, and cerebellum; while striatum appears to be most damaged. The protocol of aerobic maternal exercise was not enough to fully prevent HI-induced brain damages. PMID:26119914

  2. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    International Nuclear Information System (INIS)

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 deg. C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (YM,ult) decreased considerably from 227±23 to 107±11 ml CH4 at standard temperature and pressure (STP) conditions g-1 VSadded when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g-1 VSadded was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates

  3. Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

    Science.gov (United States)

    Brito, Paula; Antunes, Fernando

    2014-10-01

    The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity towards H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underline the interplay between oxidative stress and redox signaling responses.

  4. Aluminium-induced changes in hemato-biochemical parameters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid.

    Science.gov (United States)

    Yousef, Mokhtar I

    2004-06-01

    For a long time, aluminium (Al) has been considered an indifferent element from a toxicological point of view. In recent years, however, Al has been implicated in the pathogenesis of several clinical disorders, such as dialysis dementia, the fulminant neurological disorder that can develop in patients on renal dialysis. Therefore, the present experiment was carried out to determine the effectiveness of l-ascorbic acid (AA) in alleviating the toxicity of aluminium chloride (AlCl3) on certain hemato-biochemical parameters, lipid peroxidation and enzyme activities of male New Zealand white rabbits. Six rabbits per group were assigned to 1 of 4 treatment groups: 0mg AA and 0mg AlCl3/kg body weight (BW) (control); 40 mg AA/kg BW; 34 mg AlCl3/kg BW (1/25 LD50); 34 mg AlCl3 plus 40 mg AA/kg BW. Rabbits were orally administered their respective doses every other day for 16 weeks. Evaluations were made for lipid peroxidation, enzyme activities and hemato-biochemical parameters. Results obtained showed that AlCl3 significantly (PAluminium treatment caused a significant decrease in plasma total lipids (TL), blood haemoglobin (Hb), total erythrocytic count (TEC) and packed cell volume (PCV), and increased total leukocyte count (TLC) and the concentrations of glucose, urea, creatinine, bilirubin and cholesterol. Ascorbic acid alone significantly decreased the levels of free radicals, TL, cholesterol, glucose and creatinine, and increased the activity of GST, SH groups, Hb, TEC and PCV. While, the rest of the tested parameters were not affected. Also, the present study showed that ascorbic acid can be effective in the protection of aluminium-induced toxicity.

  5. Effects of supplementation of threshed sorghum top with selected browse plant foliage on haematology and serum biochemical parameters of Red Sokoto goats.

    Science.gov (United States)

    Okunade, Sunday Adewale; Isah, Olubukola Ajike; Oyekunle, Mufutau Atanda; Olafadehan, Olurotimi Ayobami; Makinde, Olayinka John

    2016-06-01

    The haematological and biochemical parameters of 24 growing Red Sokoto bucks (9.00 ± 0.25 kg body weight) fed threshed sorghum top (TST) with or without five different browse plant foliage Afzelia africana (AA), Daniellia oliveri (DO), Piliostigma thonningii (PT), Pterocarpus erinaceus (PE) and Annona senegalensis (AS) supplements were studied using a completely randomized design. All haematological parameters were (P < 0.05) lower in TST-fed goats compared with TST-supplemented goats, except for mean corpuscular haemoglobin concentration (MCHC), lymphocytes and monocytes which did not follow a particular pattern. Packed cell volume, haemoglobin and monocytes were higher for AA-supplemented goats while MCHC was reduced relative to other supplements (P < 0.05). White blood cell counts were increased in DO lambs compared to other supplements (P < 0.05). Serum proteins, creatinine, alanine transaminase and alkaline phosphate were reduced in TST compared with the supplements (P < 0.05). Cholesterol was higher for PT and AA than other diets (P < 0.05). Aspartate transaminase was reduced in TST goats relative to the supplements (P < 0.05). Except for potassium which was reduced in AS (P < 0.05), all serum major minerals were similar among diets. Results indicate that the entire browse fodder are good supplements to low-quality TST, though A. africana appears to have a better supplementary effect on haematological and biochemical parameters of the goats. PMID:27010715

  6. Haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria

    Directory of Open Access Journals (Sweden)

    A.O. Omonona

    2011-12-01

    Full Text Available The haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria was carried out with the view of establishing baseline blood health indices of this species and generating data which could be useful in the comparative physiology of turtles. A total of sixty free ranging turtles comprising juveniles and adults of both sexes were used for the study. The mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in male juvenile were significantly higher (P < 0.05 than those of the females. Nevertheless, in adult turtles, the mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in females were significantly higher (P < 0.05 than those of the males. Similarly, in juvenile turtles, the absolute heterophil, lymphocyte and monocyte counts in females were relatively higher (P < 0.05 than that of male while in adult turtles there were no significant differences (P < 0.05 in these parameters between the males and females. There were no significant difference (P < 0.05 in the values for the total protein, albumin, globulin, creatinine, serum glutamic-oxalacetic transaminase (SGOT, serum glutamic-pyruvic transaminase (SGPT and blood urea nitrogen in the males and females of both the juvenile and adult turtles. The outcome of this study presents baseline data on the haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria, which could also serve as a template for the comparative physiology of fresh and sea turtles.

  7. Stochastic analyses of field-scale pesticide leaching risk as influenced by spatial variability in physical and biochemical parameters

    Science.gov (United States)

    Loll, Per; Moldrup, Per

    2000-04-01

    Field-scale pesticide leaching risk assessments were performed by incorporating a numerical, one-dimensional, water and pesticide transport and fate model into the two-step stochastic modeling approach by Loll and Moldrup [1998]. The numerical model included first-order pesticide degradation, linear equilibrium adsorption, and plant uptake of water and pesticide. Simazine was used as a model pesticide, and leaching risk was expressed as the cumulative mass fraction of applied pesticide leached below 100 cm after 1 year. Spatial variability in soil physical and biochemical data, as well as measured meteorological data from an average and a relatively wet year, was considered for two Danish field sites: (1) a coarse sandy soil, with relatively small variability in hydraulic properties, and (2) a sandy loam, with large variability in hydraulic properties. The two-step stochastic modeling approach was used to investigate the relative impact of spatial variability in saturated hydraulic conductivity Ks, soil-water retention through the Campbell [974] soil-water retention parameter b, and pesticide sorption through the organic carbon content (OC). For the coarse sandy soil, field-scale spatial variability in OC was the single most important parameter influencing leaching risk, whereas for the sandy loam, Ks was found more important than OC. The relative impact of field-scale spatial variability in these parameters was found independent of the meteorological conditions, whereas the absolute level of leaching risk was highly dependent on the meteorological conditions. Assuming a linear dependency between pesticide half-life and OC, a unified approach to modeling simultaneous field-scale variability in biodegradation and adsorption was proposed. Leaching risk assessments based on this approach showed that the parts of the field with both low biological activity and low adsorption capacity contributed with a dramatic increase in leaching risk, and suggested that field

  8. Selected haematological and biochemical parameters of blood in rats after subchronic administration of vanadium and/or magnesium in drinking water.

    Science.gov (United States)

    Scibior, Agnieszka; Zaporowska, Halina; Ostrowski, Jarosław

    2006-08-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg.

  9. Model reduction and parameter estimation of non-linear dynamical biochemical reaction networks.

    Science.gov (United States)

    Sun, Xiaodian; Medvedovic, Mario

    2016-02-01

    Parameter estimation for high dimension complex dynamic system is a hot topic. However, the current statistical model and inference approach is known as a large p small n problem. How to reduce the dimension of the dynamic model and improve the accuracy of estimation is more important. To address this question, the authors take some known parameters and structure of system as priori knowledge and incorporate it into dynamic model. At the same time, they decompose the whole dynamic model into subset network modules, based on different modules, and then they apply different estimation approaches. This technique is called Rao-Blackwellised particle filters decomposition methods. To evaluate the performance of this method, the authors apply it to synthetic data generated from repressilator model and experimental data of the JAK-STAT pathway, but this method can be easily extended to large-scale cases.

  10. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

    OpenAIRE

    Danila Di Majo; Gabriella Schiera; Valentina Contrò; Elena Joana Armeli; Marcello Giaccone; Marco Giammanco; Marcello Traina; Antonio Palma; Patrizia Proia

    2014-01-01

    In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old) middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT)] and skeletal and myocardial markers of musc...

  11. Effect of Aqueous Stem Bark Extract of Khaya senegalensis on Some Biochemical, Haematological, and Histopathological Parameters of Rats.

    Science.gov (United States)

    Onu, A; Saidu, Y; Ladan, M J; Bilbis, L S; Aliero, A A; Sahabi, S M

    2013-01-01

    The subchronic effect of aqueous stem bark extract of Khaya senegalensis on some biochemical, haematological, and histopathological parameters of rats was investigated. The rats were divided into six groups of five rats per group. Groups I to VI were administered graded doses of 0, 400, 800, 1200, 1600, and 2000 mg/kg bw, respectively. The result of study revealed that administration of the Khaya senegalensis for twenty-eight days at the experimental dose resulted in significant (P < 0.05) increase in urea, electrolytes (Na(+), K(+)), and creatinine levels. The extract also significantly (P < 0.05) increased serum activity of ALT, AST, and ALP. The levels of protein, albumin, and bilirubin were significantly changed when compared to their control values, but they were not dose dependent. The hematological indices assayed in this study were not significantly affected at the experimental dose when compared to the control values. Histological studies of the liver showed cellular degeneration and necrosis and bile duct hyperplasia and fibrosis with lymphocytic infiltration of the hepatocyte, providing supportive evidence for discussing the biochemical findings, indicative of functional derangement. The histological architecture of the kidney and that of the heart were however preserved. The result of this study indicates that the aqueous stem bark extract of K. senegalensis may affect the cellular integrity of vital organs of the body.

  12. Evaluation of serum biochemical marker concentrations and survival time in dogs with protein-losing enteropathy

    NARCIS (Netherlands)

    Equilino, Mirjam; Théodoloz, Vincent; Gorgas, Daniela; Doherr, Marcus G.; Heilmann, Romy M.; Suchodolski, Jan S.; Steiner, JöRg M.; Burgener, Iwan A.

    2015-01-01

    Results—Serum C-reactive protein concentration was high in 13 of 18 dogs with PLE and in 2 of 18 dogs with FRD. Serum concentration of canine pancreatic lipase immunoreactivity was high in 3 dogs with PLE but within the reference interval in all dogs with FRD. Serum a1-proteinase inhibitor concentra

  13. Metal effects on histological and biochemical parameters of common rudd (Scardinius erythrophthalmus L.

    Directory of Open Access Journals (Sweden)

    Georgieva Elenka

    2014-09-01

    Full Text Available The present study aimed to evaluate the current contamination status of Topolnitsa Reservoir, which is located in a region with intensive copper mining. The reservoir has been continually contaminated with metals over the last few decades. As, Cd, Cu, Ni, Pb, and Zn concentrations were measured in surface water samples and in the liver of common rudd, Scardinius erythrophthalmus (L., in three different seasons: spring, summer, and autumn. The morphological structure of the fish liver was examined and the hepatic LDH, ALAT, and ASAT activities were measured. In general, metal concentrations in the water varied, but As and Cu were present in all three seasons at levels higher than the maximum permissible levels set by law. The metal concentrations in the fish liver were significantly higher than in the water. Histological alterations were classified as degenerative changes (granular, vacuolar, hydropic, and fatty degeneration, necrotic changes (necrobiosis - karyopyknosis, karryorehsis, and karyolyzis, and necrosis; and changes in blood vessels (hyperemia in sinusoids and major blood vessels. Higher LDH, ALAT, and ASAT activities in fish livers were measured in comparison to reference fish. In addition, the ALAT activity in the livers of S. erythrophthalmus from Topolnitsa Reservoir was significantly higher in the summer. Overall, it can be concluded that the metal-contaminated waters of Topolnitsa Reservoir lead to negative changes in the common rudd tissues at the cellular level including impaired structure and enhanced enzymatic activity in the fish liver.

  14. Use of simple hematological, biochemical and clinical parameters to monitor response of multiple myeloma patients on high dose thalidomide therapy

    Directory of Open Access Journals (Sweden)

    Uppal G

    2005-01-01

    Full Text Available BACKGROUND: Evidence of increased bone marrow vascularity in multiple myeloma (MM has led to the use of anti-angiogenic drugs especially thalidomide in relapsed or refractory patients. Currently, parameters such as serum/ urine electrophoresis for M (monoclonal proteins, bone marrow biopsy with touch preparation and b2 microglobulin are routinely used to assess response to therapy. These investigations are expensive, invasive and require high technical setup. AIM: To correlate simple and routine hematological and biochemical parameters with the key marker of disease i.e. M proteins. SETTINGS AND DESIGN: This is an open label, uncontrolled, single-arm study. MATERIALS AND METHODS: Twenty nine refractory or relapsed multiple myeloma patients of both sexes (M=20, F=9 with age ranging between 35-72 years were initiated on 200 mg/day of thalidomide with fortnightly increments of 200 mg to a maximum tolerated dose not exceeding 800 mg/day. All hematological and biochemical parameters were monitored at monthly intervals for one year. STATISTICAL ANALYSIS: Correlation analysis was performed between hemoglobin (Hb, total leukocyte count (TLC, absolute neutrophil count (ANC, platelet count (PC, total proteins (TP, serum albumin and serum globulin on one hand and M protein levels on the other using Pearsons Correlation test by SPSS version 7.5. RESULT: Hb, TLC, ANC, PC and serum albumin levels showed a significant negative correlation with M proteins. A highly significant positive correlation existed between M proteins on one hand and TP and globulin levels on the other. Dryness of skin indicated positive response to therapy. These correlations were found to be significant at the end of one month of therapy in all the above-mentioned parameters except in TLC where it was significant after 2 months of thalidomide therapy. CONCLUSION: Results suggest that sustained efficacy of thalidomide therapy may be amenable to monitoring by these simple, inexpensive and

  15. Toxic Effects of Lead on Growth and Some Biochemical and Ionic Parameters of Sunflower (Helianthus annuus L. Seedlings

    Directory of Open Access Journals (Sweden)

    Hamid Noorani Azad

    2011-07-01

    Full Text Available Lead (Pb is one of the non essential and toxic heavy metals which can cause oxidative stress in plants. The effects of Pb(NO32 toxicity on growth and some biochemical parameters of record cultivars of Helianthus annuus L. were studied under hydroponic condition. Different treatments of Pb(NO32 [control (0, 200, 400, 600 and 800 :M] were used in order to consider changes in dry weight, proline and Pb accumulation in roots and shoots; Total chlorophyll, enzyme activity (catalase and peroxidase and K+, Ca2+ amounts of leaves. Compared with the control, Pb treatment caused a significant decrease in roots and shoots dry weight, leaves chlorophyll, catalase activity and K+, Ca2+ amounts. In contrast, a significant increase in proline and Pb accumulation of roots and shoots and peroxidase activity of leaves was observed in Pb treatments.

  16. Effect of dietary cation-anion balance on blood biochemical parameters and items in urine of dairy cows

    International Nuclear Information System (INIS)

    Twenty cows in mid-lactation were randomly ground into 4 diets with dietary cation-anion balance (DCAB, mEq/100 g dry matter) values of 87 (control), 300, 400 and 500 to measure the effect of DCAB on blood biochemical parameters and urine index. In blood, luteotropin significantly increased at DCAB 400 fed sample, estradiol (E2) and urea decreased at DCAB 400 and 500 fed sample. However, Lactate dehydrogenate, creatine phosphokinase and total protein had a significant increase for DCAB 300 and 400 fed sample. Calcium for all treatments significantly increased. In urine, Urea and pH significantly increased, but K+ reduced at DCAB 400 and 500 fed sample. Cl- was lower for all treatments, and Na+ was higher for DCAB 300 and 500 fed sample. Urea was enhanced at DCAB 400 feed. DCAB 400 is suited as the feed for midlactation cows. (authors)

  17. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

    Directory of Open Access Journals (Sweden)

    Danila Di Majo

    2014-12-01

    Full Text Available In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT] and skeletal and myocardial markers of muscle damage [creatin kinase (CK and creatin kinase MB (CK-MB]. Endurance training, as it implies high oxygen consumption, should increase reactive oxygen species, but it has been shown that exercise leads to increased activation of antioxidant defenses. In fact, serum levels of γ-GT enzyme and total CK were not increased. On the other hand, a statistical significant reduction of CKMB has been observed. There were not variations in hematological parameters. As far as the anthropometric value is concerned, after two months of training there was a change in weight (P<0.0001. Finally, any oxidative and biological stress was highlighted in the middle distance runners but, since this is a preliminary study, it would be of interest to replicate the study on a larger sample.

  18. The Effect of Deep Water Aqua Treadmill Training on the Plasma Biochemical Parameters of Show Jumpers

    Directory of Open Access Journals (Sweden)

    Anikó Vincze

    2013-09-01

    Full Text Available Aqua treadmill is mainly used for rehabilitation purposes, but research indicates that this equipment could be used for training as well. The few studies performed with aqua treadmill mainly followed lactate and heart rate changes. Therefore, the aim of this study was to test the effect of high water treadmill training on several blood parameters and on the correlations between them. Eight similarly trained Standardbred show jumper horse competing at the same level were selected with age between 6 to 11 years. The horses were subjected to a one week standardized exercise test which included normal training, training with show jumping and aqua treadmill training. The aqua treadmill training consisted of a 10 min walk (filling up, 4.5 km/h, 30 min trot (13 km/h and 4 min walk (emptying, 4.5 km/h. Blood samples were taken from the jugular vein before aqua training, at the completion of each work bout, after drying and after one and two hour rest. Blood plasma were separated and lactate, LDH, CK, AST, glucose, cholesterol, triglyceride, total-bilirubin and cortisol level were determined. In conclusion plasma lactate response itself does not reflect correctly the intensity of workload in high water level aqua training, therefore measurement of several blood parameters is advisable. Further studies needed to understand the relationship of metabolic processes altered due to the effect of partial water submersion.

  19. Analysis Of Clinical, Haematological And Biochemical Parameters In Patients With Infectious Mononucleosis

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    Canović Petar

    2015-12-01

    Full Text Available Primary infection with Epstein-Barr virus (EBV usually occurs in early childhood and often does not present clinical symptoms. More than 90% of adults are infected with this virus. A primary infection that occurs in adolescence or adulthood is usually clinically presented as infectious mononucleosis with a triad of symptoms: fever, lymphadenopathy and pharyngitis. Our retrospective study included 51 patients with a median age of 17 (9-23 years and serologically confirmed infectious mononucleosis. All patients with infectious mononucleosis were treated at the Clinic for Infectious Diseases at the Clinical Center in Kragujevac during 2013. We analysed the clinical, haematological and laboratory parameters of patients. The aspartate-aminotransferase levels were increased in 40 patients, with a mean value of 116.24 (±93.22; the alanine-aminotransferase levels were increased in 44 patients, with a mean value of 189.24 (±196.69. Lymphadenopathy was the most common clinical feature upon admission in 49 patients (96%; 38 patients (74.5% had splenomegaly, and 20 (39% had hepatomegaly. Twenty-six patients (51% had leukocytosis with lymphocytosis, while 15 (75% of the 20 who had a normal leukocyte count also had lymphocytosis. In the present study, we updated the clinical, haematological and laboratory parameters, which may lead to the establishment of an accurate diagnosis and promote further treatment of the patients.

  20. Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system.

    Science.gov (United States)

    Bubel, F; Dobrzański, Z; Gaweł, A; Pogoda-Sewerniak, K; Grela, E R

    2015-01-01

    An influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C - standard feeding), and experimental, supplemented with prepara- tions: E-1 herbal-humic, E-2 humic-herbal and E-3--humic-lucerne. Hens were placed in the pens on the 16th week of life, addition of preparations with standard food mixture started at the 22nd wk and lasted until 66th wk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65th wk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminot- ransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne prepara- tion affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

  1. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries

    Directory of Open Access Journals (Sweden)

    A Kaur

    2012-01-01

    Full Text Available Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.

  2. Evolution of biochemical parameters in irradiated fishes: Serum proteins and intestinal nucleic acids

    International Nuclear Information System (INIS)

    In sublethal gamma-irradiated C. auratus, a sudden decrease of total serum protein concentration and a preferential descent of the low molecular weight gamma-globulin fraction have been observed. These effects are transient and after different latent periods dependent on doses, normal values are recovered, A temporal failure of a vascular permeability regulation system is probably implied. The DMA depolymerization. observed in the intestine indicates the action of radio-induced DNA degradation mechanisms since this effect is independent on doses. (Author) 29 refs

  3. Association of Apo-E gene polymorphism with biochemical and lipid metabolism parameters in patients with diabetic nephropathy of Hui and Han populations in Gansu Province

    Institute of Scientific and Technical Information of China (English)

    郭茜

    2006-01-01

    Objective To study the association of apolipoprotein (Apo) E gene polymorphism with difference in biochemical metabolism of diabetic nephropathy of Hui and Han populations. Methods ApoE genotype was determined by PCR-RFLP in diabetic patients with or without diabetic nephropathy (DN) and normal peoples in Hui and Han peoples, the related biochemical parameters were simultaneously detected. Results (1) Huis had 3 genotypes, i. e. E2/E3, E3/E3 and E3/E4, and their fre-

  4. Prematurity and Related Biochemical Outcomes: Study of Bone Mineralization and Renal Function Parameters in Preterm Infants

    Directory of Open Access Journals (Sweden)

    Sarika Singh Chauhan

    2011-01-01

    phosphorus levels were found to be significantly decreased, and serum ALP, creatinine, and electrolytes were found to be significantly increased (<0.001 at 28–30 weeks as compared to controls, but serum calcium and phosphorous levels were found to be insignificantly decreased, whereas serum ALP activities were found to be insignificantly increased at 28–30 weeks as compared to 30–32 weeks of gestational age in preterm babies. It can be concluded that high serum ALP activity and low serum calcium and phosphorus levels are associated with preterm babies. A significant difference in the mean values of these renal function parameters was also obtained, except for serum sodium and potassium.

  5. A New Data Analysis System to Quantify Associations between Biochemical Parameters of Chronic Kidney Disease-Mineral Bone Disease.

    Directory of Open Access Journals (Sweden)

    Mariano Rodriguez

    Full Text Available In hemodialysis patients, deviations from KDIGO recommended values of individual parameters, phosphate, calcium or parathyroid hormone (PTH, are associated with increased mortality. However, it is widely accepted that these parameters are not regulated independently of each other and that therapy aimed to correct one parameter often modifies the others. The aim of the present study is to quantify the degree of association between parameters of chronic kidney disease and mineral bone disease (CKD-MBD.Data was extracted from a cohort of 1758 adult HD patients between January 2000 and June 2013 obtaining a total of 46.141 records (10 year follow-up. We used an advanced data analysis system called Random Forest (RF which is based on self-learning procedure with similar axioms to those utilized for the development of artificial intelligence. This new approach is particularly useful when the variables analyzed are closely dependent to each other.The analysis revealed a strong association between PTH and phosphate that was superior to that of PTH and Calcium. The classical linear regression analysis between PTH and phosphate shows a correlation coefficient is 0.27, p<0.001, the possibility to predict PTH changes from phosphate modification is marginal. Alternatively, RF assumes that changes in phosphate will cause modifications in other associated variables (calcium and others that may also affect PTH values. Using RF the correlation coefficient between changes in serum PTH and phosphate is 0.77, p<0.001; thus, the power of prediction is markedly increased. The effect of therapy on biochemical variables was also analyzed using this RF.Our results suggest that the analysis of the complex interactions between mineral metabolism parameters in CKD-MBD may demand a more advanced data analysis system such as RF.

  6. Effects of dietary administration of stinging nettle (Urtica dioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila.

    Science.gov (United States)

    Ngugi, Charles C; Oyoo-Okoth, Elijah; Mugo-Bundi, James; Orina, Paul Sagwe; Chemoiwa, Emily Jepyegon; Aloo, Peninah A

    2015-06-01

    We investigated effects of dietary administration of stinging nettle (Urtica dioica) on growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) against Aeromonas hydrophila. Fish were divided into 4 groups and fed for 4 and 16 weeks with 0%, 1%, 2% and 5% of U. dioica incorporated into the diet. Use of U. dioica in the diet resulted in improved biochemical, hematological and immunological parameters. Among the biochemical parameters; plasma cortisol, glucose, triglyceride and cholesterol decreased while total protein and albumin in fish increased with increasing dietary inclusion of U. dioica. Among the haematology parameters: red blood cell (RBC), white blood cell (WBC) counts, haematocrit (Htc), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and netrophiles increased with increasing dietary inclusion levels of U. dioica, some depending on the fish age. Serum immunoglobulins, lysozyme activity and respiratory burst were the main immunological parameters in the adult and juvenile L. victorianus measured and they all increased with increasing herbal inclusion of U. dioica in the diet. Dietary incorporation of U. dioica at 5% showed significantly higher relative percentage survival (up to 95%) against A. hydrophila. The current results demonstrate that using U. dioica can stimulate fish immunity and make L. victorianus more resistant to bacterial infection (A. hydrophila). PMID:25827627

  7. Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

    Directory of Open Access Journals (Sweden)

    Reka Tienken

    2015-09-01

    Full Text Available The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010 serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL, whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of energy to cows which were similar in body condition score had only minor effects on periparturient immune system function and the metabolism of those cows.

  8. INTERACTIVE EFFECTS OF SALINITY STRESS AND NICOTINAMIDE ON PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS ON FABA BEAN PLANT

    Directory of Open Access Journals (Sweden)

    Magdi T. Abdelhamid

    2013-09-01

    Full Text Available A possible survival strategy of plants under saline conditions is to use some compounds that could alleviate salt stress effect. One of these compounds is nicotinamide. The effect of exogenously application of nicotinamide with different concentrations (0, 200 and 400 mg/l on Vicia faba L. plant against different NaCl treatments (0, 50 and 100 mM NaCl was investigated at the wire house of the National Research Centre, Cairo, Egypt. Salinity stress reduced significantly plant height, dry weight of shoot, photosynthetic pigments, polysaccharides, total carbohydrates, total-N contents of shoot, seed yield, total carbohydrates & total crude protein of the yielded seeds compared with those of the control plants. In contrast, salinity induced marked increases in sucrose, total soluble sugars, total free amino acids, proline, lipid peroxidation product (MDA and some oxidative enzymes (polyphenol oxidase and peroxidase enzymes. Also, salinity stress increased Na+ contents with the decreases of other macro and micro elements contents (P, K+, Mg+, Ca2+, Fe2+, Mn2+, Zn2+ and Cu2+ of shoots and the yielded seeds of faba bean. Foliar spraying of nicotinamide alleviated the adverse effects of salinity stress through increased plant height, dry weight of shoot, photosynthetic pigments, polysaccharides, total carbohydrates, total-N contents of shoot and seed yield as well as, sucrose, total soluble sugars, total free amino acids and proline, compared with those of the corresponding salinity levels, while decreased lipid peroxidation product as MDA and the oxidative enzymes (polyphenol oxidase and peroxidase enzymes. Nicotinamide inhibited the uptake of Na+ and accelerated the accumulation of P, K+ , Mg+, Ca2+, Fe2+, Mn2+, Zn2+ and Cu2+ contents in the shoots of salt stressed plants and enhanced total carbohydrate and total crude protein percentage and solutes concentrations in seeds of salinity treated plants. 

  9. Methods of mesophyll conductance estimation: its impact on key biochemical parameters and photosynthetic limitations in phosphorus-stressed soybean across CO2.

    Science.gov (United States)

    Singh, Shardendu K; Reddy, Vangimalla R

    2016-06-01

    Despite the development of various methods, the rapid estimation of mesophyll conductance (gm ) for a large number of samples is still a daunting challenge. Although the accurate estimation of gm is critical to partition photosynthetic limitations by stomatal (Ls ) and mesophyll (Lm ) conductance and by photo-biochemical (Lb ) processes, the impact of various gm estimation methods on this is ambiguous. As phosphorus (P) starvation and elevated CO2 (eCO2 ) strongly affect photosynthetic processes, their combined effect on the proportional changes in these limitations are not well understood. To investigate this, while also evaluating distinct recent methods of gm estimation sharing few common theories and assumptions, soybean was grown under a range of P nutrition at ambient and eCO2 . Methods significantly affected gm and carboxylation efficiency (VCmax ) but not other photosynthetic parameters. In all the methods, all photosynthetic parameters responded similarly to treatments. However, the percentage difference between VCmax assuming finite and infinite gm was highly inconsistent among methods. The primary mechanism responsible for P limitation to soybean photosynthesis was not CO2 diffusion limitations but Lb comprised of reduced chlorophyll, photochemistry and biochemical processes. The eCO2 decreased Lb but increased Lm without affecting Ls across leaf P concentration. Although each method explored advances of our understanding about gm variability, they all require assumptions of varying degrees, which lead to the discrepancy in the gm values. Among the methods, the oxygen sensitivity-based gm estimation appeared to be suitable for the quick assessment of a large number of samples or genotypes. Digital tools are provided for the easy estimation of gm for some methods.

  10. Basic biochemical, hematological and hormonal parameters for monitoring the health and nutritional status in athletes

    Directory of Open Access Journals (Sweden)

    Aritz Urdampilleta

    2014-09-01

    Full Text Available Sporting competitions are becoming more demanding in terms of intensity of effort, and this means controlling all aspects that affect athletic performance. Food, hydration and supplementation, before, during and after training or competition directly affect health, body composition, performance and recovery of the athlete. The assessment of nutritional status is required for proper advising of the athlete, through blood tests to control the process of adaptation to training. The aim of this paper is to provide practical tools for dietitiansnutritionists to control the health and nutritional status of athletes, as well as monitoring their adaptation to workloads and competition periods. Performing analytical tests to control of protein metabolism, lipid profile, ions, blood tests and iron metabolism, in addition to review some hormonal parameters, may be of interest in order to observe the potential existence of overtraining states. The correct understanding and interpretation of laboratory tests (under sports doctor’s supervision will be most important and useful for dietitiansnutritionists, performing dietary and nutritional advice to athletes, because it will determine the status of the athlete and propose different individual feeding strategies depending on the training phase and response.

  11. Effect of cholesterol biosynthesis inhibitor on some biochemical parameters in normal male rats

    Directory of Open Access Journals (Sweden)

    M. S. Kalo

    2009-01-01

    Full Text Available Endogenous cholesterol acts as a precursor of testosterone and other steroids hormones, this study was conducted to evaluate if there is a counterproductive effect associated with inhibition of cholesterol biosynthesis pathway specially in high doses and the degree of these effects in normal male rats. Forty eight adult Wistar rats divided into four groups, the first is control while the remaining three groups were treated with simvastatin (cholesterol biosynthesis inhibitor in doses of 25, 50 and 100 mg.kg-1 respectively. Serum samples were observed at the baseline then every fifteen days while tissue samples were observed at day 30 and 60. Results of statistic refered to a significant decrease (p≤ 0.05 in serum total cholesterol and triglycerides (by 24 and 49% ± 3 respectively, also serum testosterone was significantly decreased (by 71% ± 2 in all groups compared to control after thirty and sixty days. The activity of alanine aminotransferase was increased (57% ± 3 versus to aspartate aminotransferase. Liver cholesterol was significantly decreased (by 72% ± 2 while testicular cholesterol was decreased except the group of 100 mg.kg-1 which in turns to elevate (61% ± 4, in addition also there was a decrease in body weight gain percentage neither the weights of liver nor testis was affected. In conclusion, the inhibition of denovo pathway of cholesterol biosynthesis negatively affects testosterone level in addition to cholesterol concentration in the tissues, body weight gain and alanine aminotransferase with no successful compensatory mechanism as related with testosterone level.

  12. Influence of vegetarian and mixed nutrition on selected haematological and biochemical parameters in children.

    Science.gov (United States)

    Krajcovicová-Kudlácková, M; Simoncic, R; Béderová, A; Grancicová, E; Magálová, T

    1997-10-01

    To evaluate the health and nutritional status of children with two different nutritional habits, the authors examined 26 vegetarians (lacto- and lacto-ovo; an average period of vegetarianism 2.8 years) and 32 individuals on mixed diet (omnivores) in the age range 11-14 years. Vegetarian children had significantly lower erythrocyte number as well as reduced levels of haemoglobin and iron compared to omnivores. The average level of iron did not reach the lower limit of the physiological range and hyposiderinemia was found in 58% of vegetarians vs 9% of omnivores. Reduced iron levels were observed in spite of increased intake of vegetable iron sources and vitamin C (which facilitates the conversion to ferro-form). This reduction can be attributed to the absence of animal iron sources with high utilizability and to lower iron utilization in the presence of phytic acid (higher intake of grains compared to omnivores). The incidence of hypoalbuminemia and hypoproteinemia in vegetarian children was 38 and 12%, respectively, compared to 0% in omnivores. The protein mixture from milk, eggs and vegetable sources is complete, but vegetarian children had significantly reduced intake of milk and dairy products. Favourable lipid and antioxidant parameters in vegetarian children reflect the optimal nutrition composition with respect to the prevention of free radical diseases. Such a nutrition results in significantly lower levels of cholesterol and LDL-cholesterol compared to omnivores and significantly higher and over threshold values of essential antioxidants--vitamin C, vitamin E/cholesterol (more effective protection against LDL oxidation), beta-carotene, vitamin A.

  13. THE VALUES OF SELECTED BIOCHEMICAL PARAMETERS IN THE BLOOD SERUM OF PIGLETS AFTER USE OF PHYTOADDITIVES

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    Janka Kotosová

    2013-06-01

    Full Text Available In our work, we focused on monitoring the enzymatic activities of LDH, AST, ALT and GLU in the blood serum of pigs (piglets from the age of 21 days old to 42 days old. Subscriptions were made once each week in the morning on an empty stomach, from the eye veny (vena ophtalmica. All experimental animals were fed with compound feed COS1, COS2, without any feed additives. Piglets were divided into two groups. The first group was the control group (n = 7 piglets and the second was the experimental group (n = 7 piglets, which was given sage (Salvia officinalis L. and oregano (Origanum vulgare L. in the form of essential oils in concentrations of 0.05%. The average results of observed enzymatic activities of the 21 day old piglets were as follows: control group of piglets: LDH – 10,3 µkat/l, AST – 0,84 µkat/l a ALT – 1,75 µkat/l a GLU -5,33mmol/l and in the experimental group the results of observed enzymatic activities were : LDH – 9,09 µkat/l, AST – 0,71 µkat/l , ALT – 0,96 µkat/l a GLU – 5,97mmol/l. The sampling was repeated when the piglets reached 42 days old and in both groups we found out that the results of enzymatic activities in LDH and AST were higher and in ALT and GLU they were lower.

  14. Biochemical response of the mussel Mytilus coruscus (Mytiloida:Mytilidae)exposed to in vivo sub-lethal copper concentrations

    Institute of Scientific and Technical Information of China (English)

    LI Yifeng; GU Zhongqi; LIU Hong; SHEN Heding; YANG Jinglong

    2012-01-01

    Many aquatic organisms are negatively affected by exposure to high copper concentrations.We investigated the biochemical response of the mussel Mytilus coruscus(Mytiloida:Mytilidae)to copper exposure.In vivo bioassays using M.coruscus and different copper concentrations were conducted.The activity of six biomarkers,namely superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP),alkaline phosphatase(AKP),glutamic-oxaloacetic transaminase(GOT)and glutamic-pyruvic transaminase(GPT)were measured.Survival rates decreased with increased copper concentrations and exposure times.The LC50values at 48,72,and 96 h exposure were 0.48,0.37,and 0.32 mg/L,respectively.Within digestive glands,CAT activity increased with increasing Cu concentrations.The activity of AKP showed no significant change,while the remaining four enzymes showed decreasing activity with increasing Cu concentrations.Within the gills,AKP activity increased when the Cu concentration was 0.05 mg/L,but showed no significant changes at higher concentrations.Activity of CAT and ACP within gills tended to decrease with increasing Cu concentration.The activity of SOD and GPT decreased at an exposure concentration of 0.2 mg/L.GOT activity within gills decreased at 0.1 mg/L and increased at an exposure concentration of 0.2 mg/L.Within the adductor muscle,AKP activity increased at 0.05 mg/L but did not change at higher exposure concentrations.ACP activity within adductor muscle tissue showed no change,while activities of CAT,GOT and GPT decreased with increasing Cu concentrations.SOD activity within the adductor muscle tissue significantly decreased at the 0.02,0.05and 0.2 mg/L exposure concentrations.Our results show tissue specific differences for the six biomarkers in for M.coruscus.Our findings provide the basis for the establishment of reference activity levels against which biomarker changes can be estimated,and are essential preliminary steps in development of in vivo bioassays.

  15. Effects of static magnetic field exposure on hematological and biochemical parameters in rats

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    Salem Amara

    2006-11-01

    Full Text Available The present work was undertaken in order to investigate the effects of static magnetic field (SMF on growth rates, hematopoiesis, plasmatic proteins levels, glucose concentration, lactate dehydrogenase (LDH and transaminases activities in male rats. Sub-acute exposure of rats during 5 consecutive days to SMF (1h/day at 128mT induced an increase of plasma LDH activity (+38%, pEste estudo foi realizado com o obejtivo de investigar os efeitos do campo magnético estático (CMS nas taxas de crescimento, hematopoiese, concentrações de proteínas plasmáticas, glicemia, da desidrogenase lática (DHL e transaminases (alanina aminotransferase-ALT e aspartato aminotransferase-AST em ratos machos. Após exposição de modo sub-agudo durante 5 dias consecutivos ao CMS (1 hora/dia, a 128mT, houve aumento em 38% na concentração de DHL (p<0.05, porém não houve mudanças nos índices hematimétricos, nas proteínas plasmáticas e nas transaminases. Duas semans após exposição ao CMS durante 30 dias consecutivos (CMS (1 hora/dia, a 128mT houve diminuição significativa das taxas de crescimento e aumento significativo das concetrações de proteínas (+62%, p<0.05, da hemoglobina (+10%, p<0.05, eritrócitos (+7%, p<0.05, leucócitos (+17%, p<0.05 e plaquetas (+10%, p<0.05. A exposição sub-crônica ao CMS induziu aumento da DHL (+43%, p<0.05, AST (+ 41%, p<0.05 e ALT (+95%, p<0.05. Em contraste não houve aumento da glicemia. Estas alterações sugerem que a exposição ao CMS possivelmente influencia a proliferação de células do sistema hematopoiético e a produção enzimática, indicando alterações teciduais.

  16. Protective Effects of Bitter Almond Kernel Oil on Some Biochemical Parameters in Brain Tissue of Diabetic Rats

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    Ersin DEMIR

    2013-06-01

    Full Text Available In this study, we aimed to determine possible protective effects of the bitter almond kernel oil extract on the lipid-soluble vitamins, cholesterol, GSH, total protein, MDA and fatty acid levels of brain tissue in the streptozotocin-induced diabetic Wistar rats. The lipid-soluble vitamins, cholesterol and other sterol levels were measured by HPLC, the fatty acid levels were measured by GC, MDA, GSH and total protein levels were measured by UV-Vis spectrophotometer. Whereas the and #945;-tocopherol and GSH levels were decreased in the diabetes (D group, these parameters were protected in the diabetes + almond oil (D+AO group when compared to control (C group. The total protein and MDA levels were increased in the D group, but their levels were not changed in the D+AO group. The palmitic acid and stearic acid levels were increased in the D and D+AO groups. The arachidonic acid and docosahexaenoic acid levels were increased in the D group, but these fatty acid levels were not changed in the D+AO group when compared to the C group. In conclusion, according to our results, bitter almond oil was shown that some positive effects on the biochemical parameters of brain tissue in the diabetic rats. This oil was protected or prevented the GSH and MDA levels in bitter almond oil extract given group, and these values were closed to control group values. [J Intercult Ethnopharmacol 2013; 2(3.000: 127-134

  17. Molecular and biochemical characterization of caffeine synthase and purine alkaloid concentration in guarana fruit.

    Science.gov (United States)

    Schimpl, Flávia Camila; Kiyota, Eduardo; Mayer, Juliana Lischka Sampaio; Gonçalves, José Francisco de Carvalho; da Silva, José Ferreira; Mazzafera, Paulo

    2014-09-01

    Guarana seeds have the highest caffeine concentration among plants accumulating purine alkaloids, but in contrast with coffee and tea, practically nothing is known about caffeine metabolism in this Amazonian plant. In this study, the levels of purine alkaloids in tissues of five guarana cultivars were determined. Theobromine was the main alkaloid that accumulated in leaves, stems, inflorescences and pericarps of fruit, while caffeine accumulated in the seeds and reached levels from 3.3% to 5.8%. In all tissues analysed, the alkaloid concentration, whether theobromine or caffeine, was higher in young/immature tissues, then decreasing with plant development/maturation. Caffeine synthase activity was highest in seeds of immature fruit. A nucleotide sequence (PcCS) was assembled with sequences retrieved from the EST database REALGENE using sequences of caffeine synthase from coffee and tea, whose expression was also highest in seeds from immature fruit. The PcCS has 1083bp and the protein sequence has greater similarity and identity with the caffeine synthase from cocoa (BTS1) and tea (TCS1). A recombinant PcCS allowed functional characterization of the enzyme as a bifunctional CS, able to catalyse the methylation of 7-methylxanthine to theobromine (3,7-dimethylxanthine), and theobromine to caffeine (1,3,7-trimethylxanthine), respectively. Among several substrates tested, PcCS showed higher affinity for theobromine, differing from all other caffeine synthases described so far, which have higher affinity for paraxanthine. When compared to previous knowledge on the protein structure of coffee caffeine synthase, the unique substrate affinity of PcCS is probably explained by the amino acid residues found in the active site of the predicted protein. PMID:24856135

  18. Alterations in Somatostatin Cells and Biochemical Parameters Following Zinc Supplementation in Gastrointestinal Tissue of St reptozotocin-Induced Diabetic Rats

    International Nuclear Information System (INIS)

    Chronic hyperglycemia in diabetes is a major causative factor of free radical generation which further leads to many secondary diabetic complications via the damage to cellular proteins, membrane lipids, and nucleic acids. Zinc is an essential trace element in all living systems and plays a structural role in many proteins and enzymes. Somatostatin is known to have inhibitory effects on various gastrointestinal functions. Therefore, we determined somatostatin protein production and secretion levels, and biochemical and light microscopical changes following zinc supplementation in the gastrointestinal tract of streptozotocin (STZ)-diabetic rats. The animals were divided into four groups: Group I: control (untreated) animals; Group II: control animals given zinc sulfate; Group III: diabetic animals; and Group IV: diabetic animals given zinc sulfate. Zinc sulfate was given to the animals by gavage at a daily dose of 100 mg/kg body weight for 60 days. Diabetes was induced by intraperitoneal (i.p.) injection of STZ in a single dose of 65 mg/kg. For histological studies, stomach and duodenum tissues were fixed in Bouin solution and sections stained with Masson’s trichrome and Periodic-Acid-Schiff. Tissue homogenates were used for protein, lipid peroxidation (LPO), glutathione (GSH), and nonenzymatic glycosylation (NEG) analyses. Zinc supplementation to the STZ-diabetic rats revealed the protective effect of zinc on these parameters. Zinc supplementation may contribute to prevent at least some complications of diabetes mellitus

  19. Correlations between blood lead levels and some physiological and biochemical parameters of nutritional importance in some Nigerian women

    International Nuclear Information System (INIS)

    Lead is one of the common toxic materials widely occurring in the Nigerian environment. Even with the change to unleaded petrol, other significant sources still remain to be addressed. The deleterious impact of lead on human health, is well documented. The effects range from attention-grabbing mass mortality - as happened in Zamfara State recently, to no less serious but grossly neglected neurocognitive and neurodevelopmental effects, including lowered intelligence quotient scores. All these impacts are most serious in children, especially fetuses who receive their burden from their mothers. Ninety percent of lead in most adults resides in the bones with a half-life measured in decades. Therefore, fetuses and breast-fed children in this generation will remain seriously at risk of exposure to damaging lead levels no matter the efforts to rid our external environment of lead in the years to come. In this work, we have investigated the correlation between Blood Lead Levels (BLL) and up to 35 physiological and biochemical parameters of nutritional importance in 62 women of child-bearing age from lIe-lfe, Nigeria. BLL was determined in venous blood using Inductively- coupled plasma Mass Spectrometry. Our results show that BLL significantly correlates with Packed Cell Volume (PCV), Creatine Clearance, and the ratio of Low-density Lipids to High density Lipids in these women. When the subjects were stratified into different Nutritional Status group based on their Body Mass Index, significant correlation was found between BLL and Age but only in Obese subjects.

  20. Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats

    International Nuclear Information System (INIS)

    Nutritional Habits have changed significantly and junk foods have become widely popular, in recent years. The present study aimed to shed the light on the effect of potato chips and / or ketchup consumption on some biochemical parameters. Sixty four male and female albino rats were used in the study. Animals were maintained on 0.25 g potato chips/ rat and / or 0.125 g ketchup / rat, 5 days a week for 4 weeks. Potato chips showed the lowest body wt gain in the male rats after 4 weeks but, ketchup modulated this negative effect of the potato chips in the group of male animals fed on potato chips plus ketchup. Potato chips significantly decreased brain serotonin, liver glutathione (GSH) and catalase (CAT) in both sexes; brain dopamine, serum total proteins, albumin, total globulins, α2- and β1-globulins in the females and serum thyroxine (T4) in the male rats. Ketchup apparently affected serum T4 and A / G ratio in both sexes, brain dopamine and liver GSH in the males in addition to brain serotonin, serum total globulins and ?1-globulin in the female rats. Potato chips plus ketchup significantly changed T4, dopamine, GSH, CAT, α1 and α2-globulins in both sexes; serotonin and β1-globulin in the male rats, total proteins and albumin in the females. It could be concluded that potato chips consumption might induce numerous adverse effects in various body organs

  1. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-09-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue. PMID:23008814

  2. Influence of thyroid hormones on biochemical parameters of liver function: a case-control study in North Indian population

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    Jayashree Bhattacharjee

    2013-01-01

    Full Text Available Normal level of thyroid hormones is important for normal hepatic function and thyroid dysfunction may modulate metabolic function of liver. The purpose of this study is to determine whether liver function is associated with subclinical and overt hypothyroidism. Thyroid and liver function tests were evaluated in 47 patients with overt (TSH ≥10.0 mIU/L and 77 patients with subclinical hypothyroidism (TSH 6.0-9.9mIU/L and compared with 120 age-matched euthyroid controls. Subjects with overt hypothyroidism had significantly raised serum ALT, AST, ALP and total protein levels as compared to controls whereas subclinical hypothyroid patients had significantly increased levels of serum ALT, ALP and total protein. Further, TSH showed significant positive correlation with AST and ALP values whereas fT3 and fT4 had a negative correlation with AST in overt hypothyroidism. Thus, overt hypothyroid state is associated with significant derangement in biochemical parameters of liver function. Hence, liver function should be regularly monitored in hypothyroid patients.

  3. Evaluation of biochemical parameters and genetic markers for association with meat tenderness in South African feedlot cattle.

    Science.gov (United States)

    Frylinck, L; van Wyk, G L; Smith, T P L; Strydom, P E; van Marle-Köster, E; Webb, E C; Koohmaraie, M; Smith, M F

    2009-12-01

    A large proportion of South African feedlot cattle are crossbreds of Brahman (BrX, Bos indicus), and Simmental (SiX, Bos taurus). A sample of 20 grain fed bulls from each of these crossbreeds was used to compare meat quality with that of the small frame indigenous Nguni (NgX, Sanga) by evaluating a variety of biochemical and genetic parameters previously shown to be associated with meat tenderness. Shear force values were generally high (5.6kg average at 14days post mortem), with SiX animals higher than BrX or NgX (P=0.051) despite higher calpastatin:calpain ratio in BrX (P<0.05). Calpain activity and cold shortening were both correlated with tenderness for all classes. The sample size was too small to accurately estimate genotypic effects of previously published markers in the CAST and CAPN1 genes, but the allele frequencies suggest that only modest progress would be possible in these South African crossbreds using these markers. PMID:20416642

  4. Growth Parameters, Photosynthetic Performance, and Biochemical Characterization of Newly Isolated Green Microalgae in Response to Culture Condition Variations.

    Science.gov (United States)

    Jazzar, Souhir; Berrejeb, Nadia; Messaoud, Chokri; Marzouki, Mohamed Néjib; Smaali, Issam

    2016-08-01

    This work aimed to characterize two native microalgal strains newly isolated from South Mediterranean areas and identified as Chlorella sorokiniana ES3 and Neochloris sp. AM2. The growth properties and biochemical composition of these microalgae were evaluated in different culture media (Algal, BG-11, f/2, and Conway). Among the tested media, nitrate- and phosphate-rich Algal medium provided the maximum biomass productivities (85.5 and 111.5 mg l(-1) day(-1) for C. sorokiniana and Neochloris sp., respectively), while the nitrate- and phosphate-deficient f/2 medium resulted in the highest lipid productivities (24.1 and 35.8 mg l(-1) day(-1) for C. sorokiniana and Neochloris sp., respectively). The physiological state of both microalgae was investigated under different light and temperature levels using the pulse amplitude-modulated fluorometry. The better photosynthetic efficiency of C. sorokiniana was obtained at 23 °C with a light saturation of 156 μE m(-2) s(-1), while that of Neochloris sp. was achieved at 15 °C with a light saturation of 151 μE m(-2) s(-1). The analysis of fatty acid profile and biodiesel parameters revealed that C. sorokiniana, cultivated in Algal and f/2 media, can be considered as a suitable candidate for high-quality biodiesel production. PMID:27052210

  5. Changes in Biochemical, Strength, Flexibility, and Aerobic Capacity Parameters after a 1700 km Ultraendurance Cycling Race

    Directory of Open Access Journals (Sweden)

    Vicente Javier Clemente-Suarez

    2014-01-01

    Full Text Available The purpose of the present research was to study the organic response after ultraendurance cycling race. Selected biochemical, leg strength, flexibility, and aerobic capacity parameters were analyzed in 6 subjects 5 days before and 5 days after completing a 1700 km ultraendurance cycling race. After the race, participants presented a significant decrease in Hb (167.8 ± 9.5 versus 141.6 ± 15.7 mg/dL, strength (29.4 ± 2.7 versus 25.5 ± 3.7 cm in a countermovement jump, and oxygen uptake and heart rate at ventilatory threshold (1957.0 ± 458.4 versus 1755.2 ± 281.5 mL/kg/min and 140.0 ± 9.7 versus 130.8 ± 8.3 bpm, resp.. Testosterone presented a decrease tendency (4.2 ± 2.5 versus 3.9 ± 2.6 ng/L in opposition to the increase tendency of cortisol and ammonium parameters. Transferrin and iron levels presented high values related to an overstimulation of the liver, a normal renal function, a tendency to decrease flexibility, and an increase in aerobic capacity, finding a tendency to increase the absolute maximal oxygen uptake (37.2 ±2.4 versus 38.7 ± 1.8 mL/min in contrast to previous studies conducted with subjects with similar age. These results can be used to program training interventions, recovery times between probes, and nutritional and/or ergonomic strategies in ultraendurance events.

  6. Hatch ability, growth and some blood biochemical parameters in hatching quails following the exposure of incubated eggs to red laser

    International Nuclear Information System (INIS)

    A total number of 600 Japanese quail eggs were used this study. The eggs were divided into three groups then incubated. Eggs of groups one (G1) and two (G2) were exposed to 30 and 60 minutes, respectively, to the red laser rays (Laser Pointer LD-300) at 0, 7 and 14 days of embryogenesis,while group three (G3) was not treated and served as control. Hatch ability body weight and mortality were recorded weekly for each group. Furthermore, five blood samples were collected from one day old chicks at hatching and at six weeks of age from each group to determine some hematological parameters including RBCs, WBCs, PCV and Hb. Liver enzyme activities (GPT and GOT), kidney function (uric acid and creatinine), cholesterol and total lipids were determined for the 6 weeks old chicks only. The present results indicated that exposure to red to significant increase in hatch ability percentage and promoted early hatch ability and decreased embryonic mortality in treated groups (G1) and (G2) than control (G3). Significant decreases in RBCs counts, cholesterol and total lipids were found in G1 and G2. Exposure of developing embryos to red laser radiation had no significant effect on PCV in one day old hatching chicks, or RBCs and WBCs counts, PCV, GOT, uric acid creatinine in six weeks old birds. At the same time, significant increases in Hb and WBCs counts were occurred in one day old chicks and Hb and GPT in six weeks old birds. It could be concluded from the reported findings that exposure to red laser radiation may affect some factors that accelerate hatch ability and cause changes in some biochemical parameters in Japanese quails

  7. Roach (Rutilus rutilus) reproductive cycle: a study of biochemical and histological parameters in a low contaminated site.

    Science.gov (United States)

    Geraudie, Perrine; Gerbron, Marie; Hill, Elisabeth; Minier, Christophe

    2010-09-01

    Fish reproduction is subjected to worrying trends in many aquatic environments. In this study, we report the absence of histological and biochemical alterations in fish sampled in a low contaminated site (characterised by the absence of detectable oestrogenic activity and mutagenicity in sediment extracts). A total of 474 roach (Rutilus rutilus) were monthly sampled during 18 months, and no intersex fish were recorded after careful histological examination, thus indicating that the incidence of this phenomenon may be very low under natural conditions. Furthermore, mean male plasma vitellogenin concentration was 24 ng ml(-1) and was only slightly elevated during the spawning period (up to 120 ng ml(-1)) indicating that these low values may be characteristic of a low contaminated site. Of the male roach, 45.3% were sampled, a sex-ratio that did not significantly deviated from the expected 1:1 ratio between male and female. Results also showed that natural conditions can greatly affect the reproductive cycle of roach. Gametogenesis showed a biphasic pattern with first gonad maturation between September and December and a final maturation occurring at the end of winter/early spring. Under decreasing temperatures, particularly below 6 degrees C, gametogenesis was stopped or even regressed with secondary oocytes becoming rare under histological observation. Conversely, elevated temperatures during the winter lead to an earlier gonad maturation. PMID:19680761

  8. Association of Helicobacter pylori IgG antibody with various demographic and biochemical parameters in kidney transplant recipients

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    Azar Baradaran

    2011-01-01

    Full Text Available Few reports are available regarding the promoting factors that affect Helicobacter pylori (H. pylori infection in renal transplant (RTx patients. We report a cross-sectional study that was conducted on a group of stable RTx patients to investigate the relationship of various demographic and biochemical parameters of these patients with serum H. pylori IgG antibody titer as a sign of H. pylori infection. A total of 72 patients who were referred to the clinic for continuing their treatment were enrolled in this study. These patients included 47 males and 25 females. The mean age of the study patients was 44 (±12 years. The mean length of time after they received a transplanted kidney was 67.5 (±42 months (median: 62 months. The mean value of serum H. pylori-specific IgG antibody titer among these patients was 3 (±4.6 U/mL (median: 1 U/mL, and that of intact parathormone (iPTH was 18.4 (±8.2 pg/mL (median: 16.5 pg/mL. The mean serum magnesium (Mg was 1.9 (±0.20 mg/dL (median: 1.9 mg/dL and the mean creatinine clearance was 53 (±11 mL/min (median: 56 mL/min. In this study population, there was no significant difference in the H. pylori IgG antibody titers, serum iPTH, Mg, calcium, alkaline phosphatase and albumin levels as well as body mass index (BMI between males and females or diabetics and non-diabetics. There was no significant relationship between serum H. pylori IgG antibody titers and the age of the patients, BMI, serum Alb, phosphorus, Ca, serum leptin and serum ALP. Significant negative correlation between serum H. pylori IgG antibody titers and serum Mg (r = -0.30, P = 0.01 and serum iPTH (r = -0.25, P = 0.03 was seen. A significant positive correlation was found between serum H. pylori IgG antibody titer and creatinine clearance (r = 0.26, P = 0.02, and a near-significant positive correlation was found with the duration of RTx (r = 0.20, P = 0.08. Our study shows that the correlation of H. pylori IgG antibody titer with some

  9. Researches Concerning Reference Values Assessment of Serum Biochemical Parameters in some Fish Species from Acipenseridae, Cyprinidae, Esocidae and Salmonidae Family

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    Marioara Nicula

    2010-05-01

    Full Text Available The purpose of this work was to assess reference values of serum biochemical indices (enzymes, metabolites and minerals in some representative fish species belonging to Acipenseridae, Cyprinidae, Esocidae and Salmonidae family in order to establish a baseline data which will serve to monitoring nutritional-metabolic balance and healthy condition of these species from aquatic biocenosis or intensive exploitations. Serum samples were analyzed for glucose, total proteins, albumine, urea nitrogen, creatinine, uric acid, triglycerides, cholesterol, ALT, AST, GGT, alkaline phosphatase, amylase, total bilirubin, Ca, P, Mg, Fe, using a FullyVet automated chemical analyser. The obtained results were compared with those from other papers regarding serum biochemical profile of fresh water fish species. Serum biochemical reference intervals were as follows: glucose, 28.41±0.80–64.00±1.41 mgdL-1; total proteins, 2.78±0.21-4.05±0.20 gdL-1; albumine, 0.67±0.12–1.59±0.11 gdL-1; urea nitrogen, 12.16±0.78–18.30±0.27 mgdL-1; creatinine, 0.06±0.01–0.27±0.00 mgdL-1; uric acid, 1.00±0.00–1.66±0.09 mgdL-1; triglycerides, 46.00±1.29– 351.3±12.3 mgdL-1; cholesterol, 123.00±2.12–198.00±0.91±mgdL-1; ALT, 15.00±0.91–32.00±1.29 IU L-1; AST, 30.00±0.91–92.00±1.47 IU L-1; GGT, 4.00±0.00–6.33±0.46 IU L-1; alkaline phosphatase, 60.33±1.20–109.50±3.05 IU L-1; amylase, 28.08±0.93 – 36.00±0.91 IU L-1; total bilirubin, 0.00±0.00–0.03±0.00 mgdL-1; Ca, 7.63±0.40– 12.36±0.50 mgdL-1; P, 11.83±0.35-30.48±0.26 mgdL-1; Mg, 1.80±0.14–3.88±0.21mgdL-1; Fe, 57.60±3.48–120.00± 1.08 gdL-1. The wide intra- and interspecific variability of our data requires subsequent studies of the endo- and exogenous factors (living condition, season, age, gender, origin, breeding system, physiological and nutritional status, genetic of each individual, etc. that can induce variations of the propound parameter.

  10. Methane emissions and estimates of ruminal fermentation parameters in beef cattle fed different dietary concentrate levels

    Directory of Open Access Journals (Sweden)

    Márcio dos Santos Pedreira

    2013-08-01

    Full Text Available Using sorghum silage, the effect of roughage/concentrate ratios was evaluated on nutrient intake, digestibility, ruminal parameters and methane production by beef cattle. Three treatments (0, 30 and 60% of concentrate in DM of the diet were distributed in three Latin squares, with nine animals and three periods. Dry matter intake increased as the grain concentration in diet increased; pH showed opposite behavior. Methane emissions were lower for animals fed the diet exclusively with sorghum silage as compared with those fed 30% of concentrate, but was similar to that of animals receiving 60% of concentrate. Losses of ingested gross energy as methane were reduced by 33% when grain concentration was increased in the diet. Concentrations of propionic and butyric acids were greater in diets with grain concentrate; acetic acid concentration was not affected. Concentrate in diet increases available energy for the metabolism, measured by lower losses of ingested gross energy as ruminal methane.

  11. Effects of aqueous and ethanol extract of dried leaves of Pseudocalymma alliaceum (Bignonaceae) on haematological and biochemical parameters of wistar rats

    Institute of Scientific and Technical Information of China (English)

    Carlos Granados-Echegoyen; Rafael Prez-Pacheco; Alfonso Alexander-Aguilera; Luicita Lagunez-Rivera; Nancy Alonso-Hernndez

    2015-01-01

    Objective: To perform the toxicological evaluation of aqueous and ethanol extract of dried leaves of Pseudocalymma alliaceum (P. alliaceum) in male Wistar rats by oral administration for 14 days, and to determine the biochemical and haematological status of blood. Methods: The animals were completely randomized into four groups of three rats each. Results: No deaths were reported after oral administration of the extracts, no physical signs of toxicity or adverse effects were observed. Hematological indices of red blood cell count, hemoglobin concentration and mean corpuscular hemoglobin showed no significant abnormality; however, white series levels decrease presenting a leukopenia. Glucose, creatinine and albumin increased, while urea decreased; aspartate aminotransferase values decreased with the aqueous extract at 50 and 100 mg/kg and increased with dose of 200 mg/kg, in contrast ethanol extract caused an increase in this parameter to the doses used. The alanine aminotransferase decreased with aqueous extract and increased with ethanol extract. Triglycerides decreased when used aqueous extract and reduced with ethanol extract at 100 and 200 mg/kg, in contrast to 50 mg/kg decreased to be compared with control group. Conclusion: The daily intake of P. alliaceum did not produce acute toxicity to 50 mg/kg which may be interpreted as toxic signs or biological damage, but liver and renal function changes at dosages of 100 and 200 mg/kg; however, the reduction ability of white blood cells count could be used as a basis for specific studies on the treatment of patients with leukemia.

  12. Effects of supplementing rare earth element cerium on rumen fermentation, nutrient digestibility, nitrogen balance and plasma biochemical parameters in beef cattle.

    Science.gov (United States)

    Lin, S X; Wei, C; Zhao, G Y; Zhang, T T; Yang, K

    2015-12-01

    The objectives of the trial were to investigate the effects of supplementing rare earth element (REE) cerium (Ce) on rumen fermentation, nutrient digestibility, methane (CH4 ) production, nitrogen (N) balance and plasma biochemical parameters in beef cattle. Four Simmental male cattle, aged at 14 months, with initial liveweight of 355 ± 8 kg and fitted with permanent rumen cannulas, were used as experimental animals. The cattle were fed with a total mixed ration (TMR) composed of concentrate mixture and corn silage. Four levels of cerium chloride (CeCl3 ·7H2 O, purity 99.9%), that is 0, 80, 160 and 240 mg CeCl3 /kg DM, were added to basal ration in a 4 × 4 Latin square design. Each experimental period lasted 15 days, of which the first 12 days were for pre-treatment and the last 3 days were for sampling. The results showed that supplementing CeCl3 at 160 or 240 mg/kg DM increased neutral detergent fibre (NDF) digestibility (p cattle increased the digestibility of NDF, decreased the molar ratio of rumen acetate to propionate, increased N retention and microbial N flow and decreased CH4 /kg DMI.

  13. Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and Leporinus obtusidens (Valenciennes, 1837

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    Vania Lucia Loro

    2015-03-01

    Full Text Available Rhamdia quelen (silver catfish and Leporinus obtusidens (piava were exposed to a commercial formulation Roundup(r, a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT and aspartate aminotransferase (AST activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase activities. Plasmatic glucose levels were reduced in both species, whereas the transaminase activities (ALT and AST increased after exposure to the herbicide. Herbicide exposure increased protein and glucose levels in the mucus layer in both species. There was a reduction in both NTPDase and ecto-5'-nucleotidase activity in the brain of piava, and increased enzyme activity in silver catfish at both concentrations tested. The species showed an increase in protein carbonyl in the liver after exposure to both concentrations of the glyphosate. Our results demonstrated that exposure to Roundup(r caused liver damage, as evidenced by increased plasma transaminases and liver protein carbonyl in both of the fish species studied. The mucus composition changed and hypoglycemia was detected after Roundup(r exposure in both species. Brain nucleotide hydrolysis showed a different response for each fish species studied. These parameters indicated some important and potential indicators of glyphosate contamination in aquatic ecosystems.

  14. Detection of vitamin B12 levels with the aid of some hematological and biochemical parameters that are more sensitive

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    Süheyl Asma

    2010-09-01

    Full Text Available Vitamin B12 deficiency is a health problem, which is usually seen in elderly patients and results in hematological and neurological disorders. In this study, we aimed to investigate the serum levels of vitamin B12 could represent the real tissue levels with con-sidering some hematological and biochemical parameters. 128 patients whose vitamin B12 levels have been detected to be low and their ages are ranged from 16 to 90, have been investigated. Fifty three of them were females. Complete blood counts, antiparietal antibody(APA, homocysteine levels, peripheral blood smear evaluations and findings of gastroduodenoscopy were assessed. Mean volume of erythrocytes has been found as 107 fl in patient group (group 1, in which vitamin B12 level has been detected lower than 60 pg/mL. Thehigher homocysteine values in 85% cases, neutrophil hyper-segmentation in 82%, atrophic gastritis in 53 % and APA positivity in 47 % of cases have been detected. The average of agehas been detected as 65 in this group. High level of homocysteine has been present in 44 % of cases and MCV has been 91 fl in group 2, in which the vitamin B12 levels are between 61–100 pg/ mL. In group ( group 3, in which vitamin levels are between 101 – 140 pg /mL,elevated homocysteine levels in 27 % cases and MCV as 89 fl have been detected. In group 4, in which vitamin levels are between 141–178 pg/mL, homocysteine elevation in 16 % of cases and MCV as

  15. Effect of Ethanolic Extract of Emblica officinalis on Histopathology of Kidney and on Biochemical Parameters in Hyperlipidemic Albino Rats

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    Bheemshetty S. Patil

    2015-01-01

    Full Text Available Background: It has been reported that hyperlipidemia plays a central role in the development of atherosclerosis and oxidative stress. Embilica officinalis also known as Amla or Indian Gooseberry acts as antihyperlipidemic and antioxidant. Its active ingredients contains tannins, gallic acid and flavonoids. Aim & Objectives: It was aimed to evaluate the effect of ethanolic extract of Emblica officinalis on histopathology of kidney and on biochemical parameters in hyperlipidemic albino Wistar rats. Material and Methods: Extraction of dried fruits of Emblica officinalis was done by Soxhlet apparatus 0 using 99% ethanol at 60 C for 24 hours and also phytochemical analysis was done. Group I served as normal control. Group II was fed with isocaloric diet. Group III was fed with hyperlipidemic diet. Group IV was fed with isocaloric diet for 21 days + Embilica officinalis for 21 days. Group V was fed with hyperlipidemic diet for 21 days+ Embilica officinalis for 21 days. The dose of ethanolic extract of Emblica officinalis was taken as 100mg/kg body weight daily. Results: Percent body weight gain, kidney weight and nephro-somatic index significantly improved in hyperlipidemic rats treated with Emblica officinalis. There was a significant improvement in serum electrolyte and kidney markers. It was found that there were focal glomerular lesions with thickening of glomerulus in the kidneys of rats on hyperlipidemic diet and normal renal histology of rats on hyperlipidemic diet treated with Emblica officinalis. Conclusion: It can be concluded that Emblica officinalis may be a good, natural therapeutic agent against hyperlipidemic diet induced oxidative damage and nephrotoxicity.

  16. Effects of aqueous extract of Withania somnifera on some liver biochemical and histopathological parameters in male guinea pigs.

    Science.gov (United States)

    Al-Awthan, Yahya S; Hezabr, Samira M; Al-Zubairi, Aisha M; Al-Hemiri, Faten A

    2014-04-01

    Organophosphorus (OP) insecticides constitute one of the most widely used classes of pesticides being employed for both agricultural and landscape pest control. The purpose of this study was to investigate the effect of dimethoate (DM), an organophosphorus insecticide, on some biochemical and histopathological parameters in liver of adult male guinea pigs as well as the possible role of Withania somnifera extract in attenuation of DM-induced hepatotoxicity. The animals were divided randomly into 5 groups and kept at 5 animals per group in an environmentally controlled condition with free access to food and water ad libitum. The first group was served as a control group and administered with olive oil orally; the group II received aqueous extract of W. somnifera (100 mg kg(-1) b.wt.) orally, group III, IV and V was administered with DM (14 mg kg(-1); 1/25LD50) for 21 days orally. Group IV and V received 100 mg kg(-1) of W. somnifera extract and silymarin, respectively half hour before DM administration for 21 days. DM caused a statistically significant increase in the serum level of liver enzymes (AST, ALT, ALP) when compared to control animals, whereas, W. somnifera and silymarin pre-treatment to the DM-intoxicated animals resulted in a significant normalization of the enzymes activities. On the other hand W. somnifera extract reduced the incidence of histopathological changes such as cytoplasmic vacuolization and degeneration in nuclei, rupture of epithelia lining the central vein, widened sinusoidal space and lymphocyte infiltration induced by DM treatment in guinea pigs. In conclusion, the results of this study suggest that W. somnifera aqueous extract could protect the liver against DM-induced oxidative damage.

  17. Genetic variability in different biochemical traits and their relationship with yield and yield parameters of cotton cultivars grown under water stress conditions

    International Nuclear Information System (INIS)

    Water scarcity is an important factor limiting cotton production worldwide particularly in Pakistan. To identify drought tolerant genotypes, it is vital to understand their genetic variation for different biochemical traits under water limited conditions. In the present study, 24 genotypes of cotton (Gossypium hirsutum L.) were evaluated under two irrigation regimes viz., well watered (W1) and limited water (W2) conditions. Before physiological maturity, cotton leaves were collected and analyzed for nitrate and nitrite reductase activities, and total free amino acids. At maturity, data regarding yield and yield parameters were recorded. Significant reduction in case of all the activities of nitrate and nitrite reductase, and yield parameters was observed under W2 condition in all the genotypes; however, total free amino acids were substantially increased under W 2 condition. Correlation between the yield parameters of cotton and biochemical traits was determined. Non-significant correlation between nitrate reductase activity and yield parameters was observed under limited water condition. The genotypes evaluated exhibited decrease in the activities of nitrate and nitrite reductase whereas total free amino acids accumulation was higher under drought conditions that showed comparatively higher yield. This study shows that these biochemical traits were regulated genetically and environmentally in the tested cotton genotypes. It was concluded that these biochemical traits can be used as biochemical markers for screening cotton germplasm for drought tolerance as well as for evolving high yielding drought tolerant varieties of this crop. The findings are useful in bridging plant biochemistry and molecular biology for identifying and selecting genes involved in conferring drought tolerance in cotton. (author)

  18. Age-Dependent Variation in Hormonal Concentration and Biochemical Constituents in Blood Plasma of Indian Native Fowl

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    Avishek Biswas

    2010-01-01

    Full Text Available This experiment was to investigate the age-related changes in hormonal concentration and biochemical constituents of blood plasma in Indian native desi fowl. One hundred and sixty two (54 from each breed, i.e., Kadaknath (KN, Aseel peela (AP, and White leghorn (WLH day-old female chicks were randomly divided into nine groups each of 18 chicks (3 groups × 3 replicates. WLH was taken in this study to compare the characteristics of Indian native desi fowl. The highest level of estrogen hormone in WLH and desi fowl in blood plasma was occurred at 18 and 24 wks of age, respectively. Whereas, the peak of progesterone hormone in WLH hens noticed around 24 wks, in case of desi fowls, it was at 30 wks of age. Irrespective of the breed, the hormonal profile of Triiodothyronine (T3 and Thyroxine (T4 in blood plasma was found highest around 6 to 12 wks of age. Activities of acid phosphatase (ACP increased with the reduction of alkaline phosphate (ALP activities at different time intervals. Irrespective of the breed, transaminases (glutamic oxaloacetic transaminase (GOT and glutamic pyruvic transaminase (GPT activities of blood plasma increased linearly with the advancement of the age. From this study, it may be concluded that sexual maturity of the Indian native desi fowl occurred nearly 6 wk later (24 wk than WLH.

  19. Comparative toxicity of physiological and biochemical parameters in Euglena gracilis to short-term exposure to potassium sorbate.

    Science.gov (United States)

    Engel, Fernanda; Pinto, Luciano Henrique; Del Ciampo, Lineu Fernando; Lorenzi, Luciano; Heyder, Carmen Diamantina Teixeira; Häder, Donat Peter; Erzinger, Gilmar Sidnei

    2015-01-01

    Potassium sorbate is the potassium salt of sorbic acid, is a widespread and efficient antioxidant that has multiple functions in plants, traditionally associated with the reactions of photosynthesis; however, it has moderate toxicity to various species including rat, fish, bacteria and human health. The effects of potassium sorbate on the movement and photosynthetic parameters of Euglena gracilis were studied during short-term exposure. Potassium sorbate showed acute toxicity to the green flagellate E. gracilis affecting different physiological parameters used as endpoints in an automatic bioassay such as motility, precision of gravitational orientation (r-value), upward movement and alignment, with mean EC50 values of 2867.2 mg L(-1). The concentrations above 625 mg L(-1) of potassium sorbate induce an inhibition of the photosynthetic efficiency and electron transport rate and, in concentrations more than 2500.0 mg L(-1), the Euglena cells undergo a complete inhibition of photosynthesis even at low light irradiation. PMID:25314908

  20. Predicting Nitrate Concentration in Groundwater Resources using Lumped-Parameter Model: Case Study in Qazvin Plain

    OpenAIRE

    R.S Hajimirmohammad Ali; H Karyab

    2016-01-01

    Background and Objective: The concentration of nitrate, factors affecting the balance sheet, and the changes in an aquifer is of utmost importance. Because modeling is an efficient method to predict the concentration of ions in water resources, in this study using lumped-parameter model and Monte Carlo simulation model, the nitrate concentrations in groundwater resources of Qazvin Plain were estimated and analyzed. Materials and Methods: A total of 19 wells in different climates of saline...

  1. Methane emissions and estimates of ruminal fermentation parameters in beef cattle fed different dietary concentrate levels

    OpenAIRE

    Márcio dos Santos Pedreira; Simone Gisele de Oliveira; Odo Primavesi; Magda Aparecida de Lima; Rosa Toyoko Shiraishi Frighetto; Telma Teresinha Berchielli

    2013-01-01

    Using sorghum silage, the effect of roughage/concentrate ratios was evaluated on nutrient intake, digestibility, ruminal parameters and methane production by beef cattle. Three treatments (0, 30 and 60% of concentrate in DM of the diet) were distributed in three Latin squares, with nine animals and three periods. Dry matter intake increased as the grain concentration in diet increased; pH showed opposite behavior. Methane emissions were lower for animals fed the diet exclusively with sorghum ...

  2. Hematology and Some Biochemical Parameters of Wild Rodents in Pistachio Gardens of Kerman Province,Southeast Iran%Hematology and Some Biochemical Parameters of Wild Rodents in Pistachio Gardens of Kerman Province, Southeast Iran

    Institute of Scientific and Technical Information of China (English)

    MADJDZADEH SM; ABBASNEJAD M; TAKALLOOZADEH HM

    2011-01-01

    Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province,Southeast Iran from 2007 to 2009,then some physiological parameters of them were measured.The samples were identified as follow:Nesokia indica,Meriones persicus,Meriones lybicus and Tatera indica.Blood samples were obtained from the heart,then the blood parameters (glucose,cholesterol,triglyceride,total protein,HDL,red and white blood cell number) in wild species of rodents and laboratory rat were compared.The results showed that there were no significant differences in serum glucose,triglyceride,HDL and total protein levels among different experimental groups.The concentration of cholesterol in T.indica was more than that in N.indica (P < 0.01).The total numbers of red blood cells also showed significant difference between wild garden rodent species and laboratory rat (P < 0.01),while the numbers of white blood cells showed no significant difference.

  3. Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae

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    Juliana Castro Monteiro

    2009-04-01

    Full Text Available Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT, alanine transaminase (ALT, aspartate transaminase (AST, chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG occurred in the group that received the higher dose.As folhas de Rudgea viburnoides Benth. são utilizadas na medicina popular como diuréticas, hipotensoras, anti-reumáticas, depurativas do sangue e em regimes de emagrecimento. O presente estudo foi delineado para avaliar o efeito da infusão das folhas de R. viburnoides nos parâmetros bioquímicos plasmáticos e na morfologia e morfometria hepática de ratos Wistar adultos. Dois grupos receberam a infusão das folhas, diariamente, nas dosagens de 10 e 20 g de folhas secas/L de água, durante 40 dias. O grupo controle recebeu a mesma quantidade de água. As análises histopatológicas não mostraram áreas degeneradas e infiltrados inflamatórios. A morfometria hepática mostrou acúmulo significativo de gordura nos hepatócitos dos animais tratados, principalmente no grupo que recebeu a maior dose da infusão (8,75% de gotículas lipídicas, comparado com 0,25% delas encontradas nos animais controles. Não foram observadas alterações nos níveis plasmáticos de uréia, creatinina,

  4. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats.

    Science.gov (United States)

    Beck, W; Gobatto, C

    2016-03-01

    Nocturnal rodents should be assessed at an appropriate time of day, which leads to a challenge in identifying an adequate environmental light which allows animal visualisation without perturbing physiological homeostasis. Thus, we analysed the influence of high wavelength and low intensity light during dark period on physical exercise and biochemical and haematological parameters of nocturnal rats. We submitted 80 animals to an exhaustive exercise at individualised intensity under two different illuminations during dark period. Red light (> 600 nm; experiments.

  5. The usefulness of whole body bone mineral densitometry in the osteopenia of preterm infants: comparison with the wrist radiography and biochemical parameters

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, Bong Jin; Huh, Jin Do; Shin, Sang Bum; Cheon, Byung Kook; Joh, Young Duk [Kosin Univ. Gospel Hospital, Pusan (Korea, Republic of); Kwon, Jeong Mi; Jeon, Seong Sook [Ilsin Christian Hospital, Pusan (Korea, Republic of)

    1997-02-01

    To evaluate the usefulness of whole body bone mineral densitometry in the diagnosis of frequent osteopenia of preterm infants by comparison with the wrist radiographs and biochemical parameters. From January 1995 to January 1996, we obtained whole body bone mineral density(BMD) studies using dual energy X-ray absorptiometry(DXA) and wrist radiographs of 39 preterm infants. They were divided into three groups according to birth weight, under 1500g, 1501g to 2000g and above 2000g, and four grades of skeletal change, as seen on wrist radiography, according to the scoring method of Koo et al. Groups of birth weight and grades of skeletal change were then correlated with whole body BMD and biochemical parameters. For comparison, normal data were obtained from 13 infants born at full term. Data were analyzed by one way analysis of variation(ANOVA) and correlation and regression analysis. A p-value of less than 0.05 was considered significant. Whole body BMDs were significantly lower in the more premature and smaller birth weight infants(r=0.77, p=0.0000), and in the higher grade of skeletal change(r-0.5276, p=0.0000). Aggravated skeletal changes were found in infants with lower birth weight(r= -0.3822, p=0.01). Interobserver variation in grading skeletal change was 42.9%, and intraobserver variation was 18.4%. Biochemical parameters such as serum calcium, phosphate, alkaline phosphatase, parathromone, calcitonin and 25-hydroxy-vitamine D did not vary significantly according to either birth weight or skeletal change(p > 0.05). Premature osteopenia is more effectively diagnosed by measuring whole body BMD using DXA than by grading radiographical skeletal change or by biochemical parameters.

  6. Supplementation with L-Glutamine and L-Alanyl-L-Glutamine Changes Biochemical Parameters and Jejunum Morphophysiology in Type 1 Diabetic Wistar Rats

    OpenAIRE

    da Rosa, Carlos Vinicius D.; Azevedo, Silvia C. S. F.; Bazotte, Roberto B.; Peralta, Rosane M.; Buttow, Nilza C.; Maria Montserrat D Pedrosa; Vilma A F de Godoi; Maria Raquel M Natali

    2015-01-01

    We evaluated the effects of the supplementation with L-glutamine and glutamine dipeptide (GDP) on biochemical and morphophysiological parameters in streptozotocin-diabetic rats. For this purpose, thirty animals were distributed into six groups treated orally (gavage) during thirty days: non diabetic rats (Control) + saline, diabetic + saline; Control + L-glutamine (248 mg/kg), Diabetic + L-glutamine (248 mg/kg), Control + GDP (400 mg/kg), Diabetic + GDP (400 mg/kg). Diabetes was induced by an...

  7. Comparison of some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred race horses

    Directory of Open Access Journals (Sweden)

    Bilal Tarik

    2004-01-01

    Full Text Available The aim of this study was to determine some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred racehorses fed the same diets. The diet was formulated to provide 2.31 Mcal DE/kg, and 10.96% crude protein. Total protein, lactate, calcium, phosphorus, potassium copper, cobalt and zinc were determined in serum obtained from 40 Arabian and 40 English healthy racing thoroughbred horses aged 2-3. The copper, cobalt and zinc concentrations were determined by atomic absorption, vitamin E by HPLC and the other biochemical parameters by a spectrophotometer. Mean values were 6.77 and 6.86 g/dl for total protein, 1.88 and 2.16 mg/dl for lactate 13.18 and 12.80 mg/dl for calcium, 4.35 and 4.39 mmol/l for phosphorus, 2.64 and 3.14 mmol/l for potassium, 129 and 166 μg/dl for copper, 36 and 44 μg/dl for cobalt and, 160 and 58 μg/dl for zinc in Arabian and English horses respectively, and Mean serum vitamin E levels were 2.65 and 2.81 μg/ml respectively. This study did not demonstrate a significant effect of breed on serum total protein, lactate, calcium, phosphorus, copper, cobalt and vitamin E. However, breed may have an effect on potassium and zinc concentration in Arabian and English thoroughbred racehorses (p<0.05.

  8. Protective effect of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaei, S; Nikzad, H; Taghizadeh, M; Tameh, A A; Taherian, A; Moravveji, A

    2014-10-01

    Cancer treatment with cyclophosphamide (CP) may result in reproductive toxicity as one of its side effects. The pumpkin seed is a rich natural source of antioxidant. We have assessed the possible protective efficacy of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology of CP-treated rats. Male adult Wistar rats were categorised into four groups. Group 1 served as control and received intraperitoneal (IP) injection of isotonic saline solution. Group 2 rats were treated with CP by IP injection in a single dose of 100 mg/kg body weight, only once. Group 3 and 4 received CP plus 300 and 600 mg/kg pumpkin seed extract respectively. Six weeks after treatment, sperm characteristics, biochemical parameters and histopathological changes were examined. Results showed that, sperm characteristics in CP-treated rats were significantly decreased. Biochemical analysis results showed that the co-administration of 300 mg pumpkin seed extract could increase the total antioxidant capacity (TAC) level significantly. In CP-treated rats, histopathological changes such as vacuolisation, disorganisation and separation of epididymal epithelium were observed as well. Interestingly, pumpkin seed extract could improve the above-mentioned parameters remarkably in CP-treated rats. Our findings indicated that pumpkin seed extract might be used as protective agent against CP-induced reproductive toxicity. PMID:24124763

  9. Parâmetros bioquímicos foliares das espécies Licania tomentosa (Benth. e Bauhinia forficata (Link. para avaliação da qualidade do ar Foliar analyses of biochemical parameters of Licania tomentosa (Benth. and Bauhinia forficata (Link. species for air quality assessment

    Directory of Open Access Journals (Sweden)

    Otávio Luiz Gusso Maioli

    2008-01-01

    Full Text Available Foliar analysis of biochemical parameters were carried out in order to investigate the influence of air pollutants on two tropical tree species (Licania tomentosa (Benth. and Bauhinia forfícata (Link.. Special attention was given to tropospheric ozone due to the fact that concentration levels in the region were found to be up to 140 µg m-3 for a 4 h average time, which is well above the value that can cause injuries to orchides and tobacco (59 µg m-3. Other pollutants such as nitrogen and sulphur oxides were measured and their ambient concentrations were also associated to biochemical alterations in the investigated species.

  10. Effects of medium-chain fatty acids on performance, carcass characteristics, blood biochemical parameters and immune response in Japanese quail.

    Science.gov (United States)

    Saeidi, E; Shokrollahi, B; Karimi, K; Amiri-Andi, M

    2016-06-01

    This study had the aim of evaluating the effects of medium-chain fatty acids (MCFA) on performance, carcass characteristics, some blood parameters and antibody titre against sheep red blood cells (SRBC) in quail. A total of 240 quail chicks were allotted to 4 treatments consisting of respectively 0, 1, 2 and 4 g/kg dietary MCFA. There were no significant differences in body weight, feed intake and feed conversion ratio among treatments at different stages of the experiment. MCFAs had no significant effect on breast, thigh, liver, spleen and bursa of Fabricius weight ratios. However, the relative weight of abdominal fat significantly decreased in quail receiving 0.2 and 0.4 MCFA as compared to other treatments. Concentrations of low-density lipoprotein-cholesterol (LDL), triglycerides and total cholesterol were decreased and high-density lipoprotein (HDL)-cholesterol was increased in quail chicks receiving MCFA compared with control quail chicks. The concentrations of SRBC antibody were not statistically different among treatments. It is concluded that MCFA significantly decreased LDL, triglycerides, cholesterol and abdominal fat and increased HDL in quail chicks. PMID:27074264

  11. Changes in Some Biochemical Parameters and Somatic Cell Counts in the Milk of Buffalo and Cattle Suffering from Mastitis

    OpenAIRE

    Riaz Hussain§, Muhammad Tariq Javed and Ahrar Khan*

    2012-01-01

    The study was conducted on a total of 592 buffaloes and 453 cattle in their different stages of lactation to investigate the biochemical changes occurring in milk due to mastitis. California Mastitis Test (CMT) was used to diagnose the mammary gland infection. The results revealed significant (P

  12. The Toxicological effects of fenitrothion and vitamin E as antioxidant agent on the biochemical, cytogenetic and histopathological parameters of white rats

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    Fouad Abdel Reheim* Awad Abbas Ragab * Fatma.M. Hammam

    2008-12-01

    Full Text Available Background: The use of pesticides has been increased considerably nowadays compared to the past. The hazards of using such chemical compounds have been accentuated by the sharp rise of their use in agriculture, industry, by householders and governments. Exposure to organophosphorus insecticides (OPI in agriculture is one of the occupational hazards. Fenitrothion is one of the most important OPI. The major object of the present study was to evaluated the toxicological (biochemical, mutagenic and histopathological effects of tested insecticide "fenitrothion" alone or combined to vitamin E as an antioxidant agent to decrease their toxic effect. Material and Method male albino rats were tested orally for 30 days, three doses of fenitrothion were used in absence and presence of vitamin E (1/20, 1/40 and 1/80 LD50. Results the obtained data showed marked changes in biochemical parameter, highly inhibition of AchE activity; highly significant increase in the frequency of micronucleus (PCEM in rat bone marrow cells at all doses of fenitrothion alone or combined to vitamin compared to control group. Also, the histopathological examination of liver and kidney tissues revealed high alternation in these tissues corresponding to biochemical changes. Conclusion From these results we concluded that fenitrothion exert biochemical, mutagenic and histopathological effects in white rats. In addition, vitamin E has mild role in alleviating these toxicological effects

  13. Effect of non-phytate phosphorus levels and phytase sources on the growth performance, serum biochemical and tibial parameters of broiler chickens

    Directory of Open Access Journals (Sweden)

    Xian R. Jiang

    2013-07-01

    Full Text Available A 3×3 fattorial arrangement with dietary non-phytate phosphorus (NPP levels and phytase sources (3- and 6-phytase was conducted to evaluate the effects of NPP levels, phytase sources and their possible interactions on growth performance, serum biochemical and tibia parameters of broiler chickens from hatch to 42 days of age. A total of 540 1-day-old Arbor Acres male broiler chicks were randomly allocated into nine dietary treatments, each containing 5 replicates pens with 12 birds per pen. Interaction was statistically significant in the performance till day 21 of trial, supplementation of low NPP diet decreased body weight (BW (P<0.001, depressed average daily gain (ADG (P<0.001 and deteriorated average daily feed intake (ADFI (P<0.001 over day 42. During the 8-to-21-day period, even if interaction between NPP levels and phytase sources was significant (P<0.01, BW, ADG and ADFI always increased due to dietary supplementation of phytase, with source not differing. Dietary high NPP enhanced serum calcium and P concentrations on day 21 and 42 (linear contrast, P<0.01, while decreased alkaline phosphatase (AKP activity on day 42 (linear contrast, P<0.001, and interaction was not significant. Both dietary sources of phytase decreased serum AKP activities on day 42 (P<0.001, and urea nitrogen content on day 21 (P<0.01 and 42 (P<0.001. Both phytase improved ash percentage on day 21 and P content in tibia at 21 and 42 days of age (P<0.001. The results confirmed that dietary supplementation of phytase may enhance P availability during the 8-to-21-day period. Nevertheless, no difference between the two phytase sources was observed.

  14. Predicting Nitrate Concentration in Groundwater Resources using Lumped-Parameter Model: Case Study in Qazvin Plain

    Directory of Open Access Journals (Sweden)

    R.S Hajimirmohammad Ali

    2016-03-01

    Full Text Available Background and Objective: The concentration of nitrate, factors affecting the balance sheet, and the changes in an aquifer is of utmost importance. Because modeling is an efficient method to predict the concentration of ions in water resources, in this study using lumped-parameter model and Monte Carlo simulation model, the nitrate concentrations in groundwater resources of Qazvin Plain were estimated and analyzed. Materials and Methods: A total of 19 wells in different climates of saline watershed in Qazvin Plain were selected and entry and exit routes of nitrate to these sources were analyzed using lumped-parameter model.  Finally, Monte Carlo simulation was used to determine the probability of the estimated nitrate concentration in aquifer. Results: Application of lumped-parameter model for a part of a part of groundwater resources in Qazvin Plain watershed predicted the nitrate concentration in the range of 8.12 to 15.94 mg/l.   The maximum concentration was estimated in cold-dry climate with 12.8±0.04 mg/L. Moreover, it was found that the difference between the estimated nitrate concentration and factors affecting its concentration in different climates was significant (p<0.05. Conclusion: Despite the predicted concentrations of nitrate in the study area were in accordance with the Iran national standard for drinking purposes, the cumulative probability of Monte Carlo simulation showed that the possible violation of nitrate from the safe limit of 10 mg/l in the study area is 90% (p = 0.005.

  15. The ratios of human arginase activity to other biochemical parameters in the serum of pre- and post-hemodialysis patients as new clinical indices

    Directory of Open Access Journals (Sweden)

    Hamid Derazhi

    2007-01-01

    Full Text Available Introduction: Prognosticators are indices which are used by medical specialists in early detection to make better decisions about treatment in hemodialysis patients. In this study the ratios of human arginase activity with other biochemical parameters in serum of pre- and post- dialysis patients were compared to healthy individuals. The ratios evaluated as new indices for diagnosis and treatment of the patients. Methods and Materials: In this study, 286 human serum specimens were evaluated, 206 of which belonged to the hemodialysis patients (103 of pre- and 103 of post- dialysis and 80 to the healthy individuals. Arginase activity were determined by urea method, blood urea nitrogen by urease method, uric acid by modified Folin method, creatinine by Jaffe method and total protein was measured by biuret method.Results: The results showed that the serum arginase activity of hemodialysis patients (pre- and post- dialysis was significantly higher than healthy subjects (p 0.01.Conclusion: This investigation suggests that in parallel with other serum biochemical tests, it is beneficial to measure arginase activity and using the ratios of arginase activity to other biochemical serum parameters as new indices in prognosis and treatment of hemodialysis patients.

  16. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata) and Wheat (Triticum aestivum) Seedlings

    OpenAIRE

    Mobin Afzal; Simeen Mansoor

    2012-01-01

    Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bea...

  17. The effect of sodium valproate on the biochemical parameters of reproductive function in male albino Wistar rats

    OpenAIRE

    Vijay P.; Yeshwanth R; Bairy K

    2008-01-01

    Objective: To assess the effects of sodium valproate on intratesticular testosterone and lactic dehydrogenase level in rats. Methods: Male Wistar rats (12 weeks old) were treated with sodium valproate and sacrificed at the end of the 2 nd , 4 th , 5 th , 7 th , 10 th and 15 th week, after the last exposure to sodium valproate. The testes were removed, weighed and processed for biochemical analysis. Results: The intratesticular testosterone level was significantly (P< 0.001) reduced...

  18. The relationships between air pollutants, meteorological parameters and concentration of airborne fungal spores

    Energy Technology Data Exchange (ETDEWEB)

    Grinn-Gofron, Agnieszka, E-mail: agofr@univ.szczecin.p [Department of Plant Taxonomy and Phytogeography, Faculty of Natural Science, University of Szczecin, Waska 13 Street, 71-415 Szczecin (Poland); Strzelczak, Agnieszka [Department of Food Process Engineering, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin (Poland); Wolski, Tomasz [Physical Oceanography Laboratory, University of Szczecin (Poland)

    2011-02-15

    Fungal spores are an important component of bioaerosol and also considered to act as indicator of the level of atmospheric bio-pollution. Therefore, better understanding of these phenomena demands a detailed survey of airborne particles. The objective of this study was to examine the dependence of two the most important allergenic taxa of airborne fungi - Alternaria and Cladosporium - on meteorological parameters and air pollutant concentrations during three consecutive years (2006-2008). This study is also an attempt to create artificial neural network (ANN) forecasting models useful in the prediction of aeroallergen abundance. There were statistically significant relationships between spore concentration and environmental parameters as well as pollutants, confirmed by the Spearman's correlation rank analysis and high performance of the ANN models obtained. The concentrations of Cladosporium and Alternaria spores can be predicted with quite good accuracy from meteorological conditions and air pollution recorded three days earlier. - ANN models predict airspore contents from weather conditions and air pollutant.

  19. Environmental parameters series. 3. Concentration factors of radionuclides in freshwater organisms

    International Nuclear Information System (INIS)

    This report outlines recent research activities of Radioactive Waste Management Center. Aiming to estimate the radiation dose of man exposed to radioactive materials in an environment, construction of a calculation model on the transfer of radionuclide in the environment was attempted. This issue, Environmental parameter series No.3 includes six reports on the factors related to environmental concentration for radionuclides. The title of the reports are as follows; Factors modifying the concentration factor (CF), Evaluation of accumulation of radionuclides in brackish water organisms, Dose assessment, CF derived from Japanese limnological data, Data table of CF and Metabolic parameters in relation to bioaccumulation of elements by organisms. In addition to collect and arrange the existing data, CF was calculated based on the concentration of stable elements in various lakes and rivers in Japan. (M.N.)

  20. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation.; Efeito da radiacao gama em alguns parametros microbiologicos e bioquimicos da fermentacao alcoolica

    Energy Technology Data Exchange (ETDEWEB)

    Alcarde, Andre Ricardo

    2000-07-01

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  1. Impacts of some meteorological parameters on the SO2 concentrations in the City of Obrenovac, Serbia

    Directory of Open Access Journals (Sweden)

    SNEŽANA S. NENADOVIĆ

    2010-05-01

    Full Text Available In this paper, the impacts of some meteorological parameters on the SO2 concentrations in the City of Obrenovac are presented. The City of Obrenovac is located in the north-west part of Serbia on the banks of the River Sava. The observed source emission, the power plants TENT A and B are situated on the bank of the Sava River in the vicinity of Obrenovac. During the period from January to November 2006, the concentrations of sulfur dioxide in the air at 4 monitoring sites in Obrenovac were measured. It was noticed that the maximal measured daily concentrations of sulfur dioxide ranged from 1 μg m-3 (16th November, 2006 to 98 μg m-3 (29th January 2006 and lie under the maximal allowed concentration value according to the Serbian Law on Environmental Protection. The measured sulfur dioxide concentrations mostly showed characteristics usual for a daily acidification sulfur dioxide cycle, excluding the specificities influenced by the measuring site itself. Sulfur dioxide transport was recorded at increased wind speeds, primarily from the southeast direction. Based on the impact of meteorological parameters on the sulfur dioxide concentration, a validation of the monitoring sites was also performed from the aspect of their representivity.

  2. Concentrations of Mineral in Amniotic Fluid and Their Relations to Selected Maternal and Fetal Parameters.

    Science.gov (United States)

    Suliburska, J; Kocyłowski, R; Komorowicz, I; Grzesiak, M; Bogdański, P; Barałkiewicz, D

    2016-07-01

    The concentrations of various trace elements in amniotic fluid (AF) change over the course of pregnancy, with gestational age and fetus growth. The aim of the present study was to evaluate the concentrations of selected essential and toxic elements in AF and their relations to maternal and fetal parameters. The study was carried out in 39 pregnant women, aged 34.6 ± 4.7 years, between weeks 16 and 26 of gestation. Amniotic fluid samples were obtained during the standard procedure of amniocentesis in high-risk patients for chromosomal abnormalities. An inductively coupled plasma mass spectrometry (ICP-MS) technique was used to determine the levels of Al, As, Ba, Cd, Co, Cr, Cu, Mg, Mn, Ni, Sr, U, and V in AF. Body mass and blood pressure were measured in all the women. The basic parameters of fetal development were also assayed. It was found that the age of the mother, the gender of the fetus, and the week of the pregnancy may affect the concentrations of mineral in the amniotic fluid. Moreover, several significant correlations between the essential and toxic elements and maternal and fetal parameters were observed. In particular, negative and positive correlations between fetal parameters and magnesium and copper levels in AF, respectively, were seen. The present findings demonstrate the association between minerals in AF and fetal development.

  3. Concentrations of Mineral in Amniotic Fluid and Their Relations to Selected Maternal and Fetal Parameters.

    Science.gov (United States)

    Suliburska, J; Kocyłowski, R; Komorowicz, I; Grzesiak, M; Bogdański, P; Barałkiewicz, D

    2016-07-01

    The concentrations of various trace elements in amniotic fluid (AF) change over the course of pregnancy, with gestational age and fetus growth. The aim of the present study was to evaluate the concentrations of selected essential and toxic elements in AF and their relations to maternal and fetal parameters. The study was carried out in 39 pregnant women, aged 34.6 ± 4.7 years, between weeks 16 and 26 of gestation. Amniotic fluid samples were obtained during the standard procedure of amniocentesis in high-risk patients for chromosomal abnormalities. An inductively coupled plasma mass spectrometry (ICP-MS) technique was used to determine the levels of Al, As, Ba, Cd, Co, Cr, Cu, Mg, Mn, Ni, Sr, U, and V in AF. Body mass and blood pressure were measured in all the women. The basic parameters of fetal development were also assayed. It was found that the age of the mother, the gender of the fetus, and the week of the pregnancy may affect the concentrations of mineral in the amniotic fluid. Moreover, several significant correlations between the essential and toxic elements and maternal and fetal parameters were observed. In particular, negative and positive correlations between fetal parameters and magnesium and copper levels in AF, respectively, were seen. The present findings demonstrate the association between minerals in AF and fetal development. PMID:26547910

  4. Dikegulac-Sodium Effect on Micropropagation and Biochemical Parameters in the Cherry Rootstocks CAB-6P and Gisela 6

    OpenAIRE

    SARROPOULOU, VIRGINIA; DIMASSITHERIOU, Kortessa; Therios, Ioannis

    2014-01-01

    The effects of dikegulac-sodium (DS) alone and in combination with benzyladenine (BA) οn the morphogenetic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus x Prunus canescens) were investigated. In the CAB-6P rootstock, DS did not promote shoot proliferation whereas its application at 40, 120 and 150 μΜ suppressed shoot length. In the Gisela 6 rootstock, the number of shoots per explant and shoot proliferation rate were greater with 80...

  5. Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaie, Somaieh; Nikzad, Hossein; Mahabadi, Javad Amini; Taghizadeh, Mohsen; Azami-Tameh, Abolfazl; Taherian, Aliakbar; Sajjadian, Seyyed Mohammad Sajjad; Kamani, Mehran

    2016-09-01

    Reproductive toxicity is one of the side effects of cyclophosphamide (CP) in cancer treatment. Pumpkin seeds and Zingiber officinale are natural sources of antioxidants. We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats. Male adult Wistar rats were divided randomly into six groups. Group 1, as a control, received an isotonic saline solution injection intraperitoneally (IP). Group 2 were injected IP with a single dose of CP (100 mg/kg) once. Groups 3 and 4 received CP plus 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract (50:50). Groups 5 and 6 received only 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract. Six weeks after treatment, sperm characteristics, histopathological changes and biochemical parameters were assessed. In CP-treated rats, motile spermatozoa were decreased, and abnormal or dead spermatozoa increased significantly (P < 0.001) but administration of the mixed extract improved sperm parameters. Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. Biochemical analysis showed that the administration of combined extracts could increase the total antioxidant capacity (TAC) level significantly in groups 3, 4, 5 and 6. Interestingly, the mixed extract could decrease most of the side effects of CP such as vacuolization and separation of epididymal tissue. Our findings indicated that the combined extracts might be used as a protective agent against CP-induced reproductive toxicity. PMID:26714700

  6. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish.

  7. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish. PMID:27245867

  8. Prognostic value of metabolic parameters and clinical impact of {sup 18}F-fluorocholine PET/CT in biochemical recurrent prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Colombie, M.; Bailly, C.; Rusu, D.; Rousseau, N. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Campion, L. [ICO Cancer Center, Statistics, Saint-Herblain (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, T. [Urologic Clinic Nantes-Atlantis, Saint Herblain (France); Mathieu, C. [University Hospital, Nuclear Medicine, Nantes (France); Ferrer, L. [ICO Cancer Center, Physics, Saint-Herblain (France); Kraeber-Bodere, F. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); University Hospital, Nuclear Medicine, Nantes (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, C. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France)

    2015-11-15

    To evaluate the therapeutic impact of {sup 18}F-fluorocholine (FCH) PET/CT in biochemical recurrent prostate cancer (PC) and to investigate the value of quantitative FCH PET/CT parameters in predicting progression-free survival (PFS). This retrospective study included 172 consecutive patients with PC who underwent FCH PET/CT for biochemical recurrence. Mean rising PSA was 10.7 ± 35.0 ng/ml. Patients with positive FCH PET were classified into three groups: those with uptake only in the prostatic bed, those with locoregional disease, and those with distant metastases. Referring physicians were asked to indicate the hypothetical therapeutic strategy with and without the FCH PET/CT results. Clinical variables and PET parameters including SUVmax, SUVpeak, SUVmean, total lesion choline kinase activity (TLCKA) and standardized added metabolic activity (SAM) were recorded and a multivariate analysis was performed to determine the factors independently predicting PFS. In 137 of the 172 patients, the FCH PET/CT scan was positive, and of these, 29.9 % (41/137) had prostatic recurrence, 42.3 % (58/137) had pelvic lymph node recurrence with or without prostatic recurrence, and 27.7 % (38/137) had distant metastases. The FCH PET/CT result led to a change in treatment plan in 43.6 % (75/172) of the 172 patients. Treatment was changed in 49.6 % (68/137) of those with a positive FCH PET/CT scan and in 20 % (7/35) of those with a negative FCH PET/CT scan. After a median follow-up of 29.3 months (95 % CI 18.9 - 45.9 months), according to multivariate analysis age <70 years, SAM ≥23 and SUVmean ≥3 were parameters independently predicting PFS. A nomogram constructed using the three parameters showed 49 months of PFS in patients with the best scores (0 or 1) and only 11 months in patients with a poor score (score 3). This study indicates that a positive FCH PET result in PC patients with biochemical recurrence predicts a shorter PFS and confirms the major impact of the FCH PET

  9. Influence of smoking parameters on the concentration of polycyclic aromatic hydrocarbons (PAHs) in Danish smoked fish

    DEFF Research Database (Denmark)

    Duedahl-Olesen, Lene; Christensen, J. H.; Højgård, A.;

    2010-01-01

    smoking, and for other fish species direct smoking leads to higher sigma PAH25 than indirect smoking. Also, the usage of common alder increases the PAH contamination compared with beech. The effects of smoking time, combustion temperatures, and two types of smoke-generating material on the Sigma PAH25......A new method for the analysis of 25 polycyclic aromatic hydrocarbon (PAH) compounds in fish was developed, validated, and used for the quantification of PAHs in 180 industrially smoked fish products. The method included pressurized liquid extraction, gel-permeation chromatography (Bio-beads S-X3...... concentrations were obtained for indirectly smoked trout (26 mu g kg-1). Principal component analysis was used to correlate processing parameters to PAH concentrations and to identify the effects of these parameters. The analysis showed that for salmon hot-smoking conditions lead to higher sigma PAH25 than cold...

  10. The Polyphenols Stability, Enzyme Activity and Physico-Chemical Parameters During Producing Wild Elderberry Concentrated Juice

    OpenAIRE

    Ante Galić; Verica Dragović-uzelac; Branka Levaj; Danijela Bursać Kovačević; Stjepan Pliestić; Sabina Arnautović

    2009-01-01

    The influence of processing wild elderberry into concentrated juice on polyphenols (total phenols, flavonoids, non-flavonoids, anthocyanins, flavan-3-ols, hydrolysed tannins) stability, activity of polyphenol oxidase (PPO) and peroxidase (POD), and changes of physico-chemical parameters (total and soluble dry matter, total acidity, pH, sugars) were investigated. The amounts of total phenols, flavonoids, non-flavonoids, falvan-3-ols and hydrolysed tannins were analyzed using Folin-Ciocalteu co...

  11. Blood parameters and apparent digestibility of concentrate with rice oil for horses

    Directory of Open Access Journals (Sweden)

    Helio Alberto Cumani Garcia

    2013-10-01

    Full Text Available Apparent digestibility coefficients and serum parameters were measured to evaluate the effect of supplementing feed concentrates with rice bran oil in horses. Twelve horses (6 males and 6 females with a mean age of 18 ± 4 months old and mean live weight of 306 ± 22.6 kg were used. Treatments consisted of increasing rice bran oil concentrate levels of 0, 3.5, 7.0, 10.5, 14.0 and 17.5%, considering a daily intake of 2.25% live weight on a dry matter basis. A dietary effect of supplementation on the apparent digestibility of gross energy (y = 64.55 - 0.58x was observed (P0.05. Supplementation did not affect serum glucose levels (P>0.05, but cholesterol was affected (P0.05. A dietary effect on the triglyceride (y = 15.73 - 0.96x + 0.0524x² and HDL (high-density lipoprotein (y = 45.24 + 1.0499x parameters was observed (P<0.01. While the use of rice bran oil does affect blood parameters associated with lipid metabolism, rice bran oil levels up to 17.5% concentrate do not negatively affect the apparent digestibility of dietary nutrients.

  12. The Polyphenols Stability, Enzyme Activity and Physico-Chemical Parameters During Producing Wild Elderberry Concentrated Juice

    Directory of Open Access Journals (Sweden)

    Ante Galić

    2009-12-01

    Full Text Available The influence of processing wild elderberry into concentrated juice on polyphenols (total phenols, flavonoids, non-flavonoids, anthocyanins, flavan-3-ols, hydrolysed tannins stability, activity of polyphenol oxidase (PPO and peroxidase (POD, and changes of physico-chemical parameters (total and soluble dry matter, total acidity, pH, sugars were investigated. The amounts of total phenols, flavonoids, non-flavonoids, falvan-3-ols and hydrolysed tannins were analyzed using Folin-Ciocalteu colorimetric method, while the total anthocyanins were determined by bisulphite bleaching method. Total phenols ranged from 25.87 mg/g DM to 38.87 mg/g DM. Total anthocyanins were the most abundant polyphenols in all investigated samples (raw elderberries, elderberries after blanching, elderberry juice after disintegration and pressing, concentrated elderberry juice and their concentration ranged from 13.12 mg/g DM to 25.67 mg/g DM. Other polyphenols determined in high concentration were hydrolysed tannins, followed by fl avan-3-ols, flavonoids and nonfavonoids. After blanching, the concentration of all polyphenols did not decrease significantly. After disintegration of elderberries the concentration of all polyphenols increased, probably due to inactivation of PPO and POD and better isolation of polyphenols from homogenized puree. During processing of elderberry juice into concentrated juice most polyphenols were stable. Total acidity and pH value were not changed during processing, whereas the amounts of total and reducing sugar increased after pressing and additionally after concentration. The obtained results suggest that raw elderberries as well as elderberry concentrated juice are high potential source of polyphenols especially anthocyanins.

  13. Effect of Subacute Exposure of Wrightia tinctoria Bark Extract on Hematological, Biochemical and Antioxidant Enzyme Parameters of Rat

    Directory of Open Access Journals (Sweden)

    Papiya Bigoniya

    2009-01-01

    The present study was undertaken to investigate the effect of sub-acute administration of W. tinctoria bark extract on some haematological, biochemical, histological and antioxidant enzyme status of rat liver and kidney following 21 and 45 days treatment. The animals were observed for gross physiological and behavioural responses, food and water intake and body weight changes. Free radical scavenging activity and histopathology was done on liver and kidney samples. W. tinctoria showed significant hemopoiesis with increase in body weight signifying anabolic effect. It significantly reduced serum SGOT level and increased glucose levels. W. tinctoria caused increased SOD activity of liver along with catalase of both liver and kidney and decreased liver peroxidase (P< 0.001. These features indicate that W. tinctoria upto 1000 mg/kg daily dose is safe and has potential to be consumed for long time in management of various diseases.

  14. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia.

  15. The phytoconstituents and the comparative effects of aqueous extract of Irvingia gabonensis seeds and proviron on the biochemical parameters of male guinea pigs

    Institute of Scientific and Technical Information of China (English)

    Obianime AW; Uche FI

    2010-01-01

    Objective:To investigate the phytochemical screening and the effects of the aqueous extracts of the seeds ofIrvingia gabonensis on the biochemical parameters of male guinea pigs.Methods: The biochemical parameters were assayed using Randox Diagnostic kits, Phenolphthalein method and colorimetric method. The phytochemical screening was carried out using standard procedures.Results: Phytochemical investigations revealed the presence of flavonoids, tannins, carbohydrate, alkaloids, terpenoids, steroids, volatile oils, saponins and cardiac glycosides. The aqueous extract ofIrvingia gabonensis seeds (50 - 400 mg/kg) caused a statistically significant (P< 0.05 ANOVA) decrease in the levels of total cholesterol, urea, uric acid, total protein, prostatic, alkaline, and acid phosphatases. The highest reduction effect was obtained with uric acid at 400 mg/kg ofIrvingia gabonensis extract while the least effect was observed in total cholesterol. These effects were dose- and time- dependent.Conclusions: This shows that the seeds of Irvingia gabonensis have hepatoprotective, nephroprotective and cardio protective properties. The study therefore, supports the claims on the use of the seeds of this plant by traditional medicine practitioners as a hepatoprotective and nephroprotective agent. Although further studies need to be done to isolate, identify and characterize the active principles in the seeds of this plant.

  16. Diagnostic Value of Serum Lactate Dehydrogenase Isoenzyme and Amino Acid Patterns in Several Schistosomal and Non-Schistosomal Disorders as Compared to other Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    Samia A. Ahmed

    1996-01-01

    Full Text Available Serum lactate dehydrogenase (LDH isoenzyme and amino acid (a. a patterns were evaluated in comparison to several other biochemical parameters for liver and renal function with the objective of clarifying the differential diagnosis of hepatic disorders and predicting the outcome of schistosomal infection in Egyptian patients. Patients examined included those with complicated hepatic disorders and others with different stages of schistosomal infestation, hepatoma or bladder cancer, in addition to a normal control group. Several biochemical parameters appeared to be useful in establishing consistent differences or similarities between the studied groups. Examples are; elevated serum AST/ AL T ratio and methionine content in chronic schistosomiasis, elevated serum urea/creatinine ratio and leucine content in all schistosomal patients and extremely high levels of N-acetyl-β-D-glucosaminidase (NAG in the urine of non-schistosomal bladder cancer patients. In addition, characteristic LDH isoenzyme profiles distinguish between the studied groups, in particular separating chronic schistosomiasis from schistosomal bladder cancer and hepatoma from other hepatic disorders.

  17. Assessment of serum biochemical parameters and pathological changes in broilers with chronic aflatoxicosis fed glucomannan-containing yeast product (Mycosorb and sodium bentonite

    Directory of Open Access Journals (Sweden)

    Azizpour Aidin

    2015-06-01

    Full Text Available The purpose of this study was to evaluate the efficacy of yeast glucomannan (YG and sodium bentonite (SB in reducing the toxicity in broilers fed a diet naturally contaminated with aflatoxin. Three hundred 7-day-old Ross 308 strain broilers were chosen and randomly assigned to 10 dietary treatments. Serum biochemical parameters and pathological changes in the liver were investigated at 42 d of age. Chickens fed a diet containing 250 ppb of aflatoxin displayed a decrease in uric acid, cholesterol, and triglycerides, and an increase in serum activities of AST and ALT when compared to control group. There were considerable gross and histopathological hepatic lesions (P < 0.05 in the form of small to moderate hydropic and/or fatty degeneration, bile duct hyperplasia, periportal fibrosis, cells infiltration, and congestion, in chickens fed the 250 ppb aflatoxincontaining diet. The addition of YG and SB to the aflatoxin-containing diet partially reduced the negative effects of aflatoxin. The 0.1% YG supplementation to the aflatoxin-contaminated diet significantly prevented the pathological effect of aflatoxin on serum biochemical parameters and liver, and was found to be more effective than other treatments.

  18. Effects of magnetic treated water on serum concentration parameters and fat thickness

    Directory of Open Access Journals (Sweden)

    Geraldo Balieiro Neto

    2013-02-01

    Full Text Available The goal of this study was to evaluate the effect of magnetic water on blood cells counts, biochemical profile, blood gas level and subcutaneous fat thickness of Jersey cows. This research was carried out at Agência Paulista de Tecnologia dos Agronegócios - APTA, SP. Twenty six Jersey cows from the APTA were allotted into two groups: control (n=13, drinking regular water and the group consuming magnetic water (n=13. The animals were lactating around 150 days and pregnant around 60 days. Blood samples were collected from caudal auricular artery and jugular vein. The water treatment had no effect on hemogram (p>0.05. Higher pH (7.448 vs 7.407 mmHg, p<0.05 and lower PaCO2 (37.97 vs 42.47 mmHg, p<0.05 levels were detected in arterial blood of the group drinking magnetic water. The concentration of Na ion (138.8 vs 145.5 mmol/l, p<0.05 and serum triglycerides (10.4 vs 22.6 mg/dL, p<0.05 were significantly lower, resulting in smaller osmolality (273.30 vs 280.99 mOsm/kg, p<0.05 and subcutaneous fat thickness (0.2 vs 1.3 mm, p<0.05. In summary, the water treatment, evaluated in this study, may have decreased the risk of some metabolic disorders, such as acidosis and high serum concentration of Na and carbon dioxide.

  19. Does the adequacy parameter Kt/V(urea reflect uremic toxin concentrations in hemodialysis patients?

    Directory of Open Access Journals (Sweden)

    Sunny Eloot

    Full Text Available Hemodialysis aims at removing uremic toxins thus decreasing their concentrations. The present study investigated whether Kt/V(urea, used as marker of dialysis adequacy, is correlated with these concentrations. Predialysis blood samples were taken before a midweek session in 71 chronic HD patients. Samples were analyzed by colorimetry, HPLC, or ELISA for a broad range of uremic solutes. Solute concentrations were divided into four groups according to quartiles of Kt/V(urea, and also of different other parameters with potential impact, such as age, body weight (BW, Protein equivalent of Nitrogen Appearance (PNA, Residual Renal Function (RRF, and dialysis vintage. Dichotomic concentration comparisons were performed for gender and Diabetes Mellitus (DM. Analysis of Variance in quartiles of Kt/V(urea did not show significant differences for any of the solute concentrations. For PNA, however, concentrations showed significant differences for urea (P<0.001, uric acid (UA, p-cresylsulfate (PCS, and free PCS (all P<0.01, and for creatinine (Crea and hippuric acid (HA (both P<0.05. For RRF, concentrations varied for β₂-microglobulin (P<0.001, HA, free HA, free indoxyl sulfate, and free indole acetic acid (all P<0.01, and for p-cresylglucuronide (PCG, 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF, free PCS, and free PCG (all P<0.05. Gender and body weight only showed differences for Crea and UA, while age, vintage, and diabetes mellitus only showed differences for one solute concentration (UA, UA, and free PCS, respectively. Multifactor analyses indicated a predominant association of concentration with protein intake and residual renal function. In conclusion, predialysis concentrations of uremic toxins seem to be dependent on protein equivalent of nitrogen appearance and residual renal function, and not on dialysis adequacy as assessed by Kt/V(urea. Efforts to control intestinal load of uremic toxin precursors by dietary or other

  20. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata and Wheat (Triticum aestivum Seedlings

    Directory of Open Access Journals (Sweden)

    Mobin Afzal

    2012-03-01

    Full Text Available Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bean (Vigna radiata and Wheat (Triticum aestivum seedlings. The effect of cell phone Electromagnetic Radiations (EMR were determined by measuring membrane damage in terms of melondialdehyde [MDA] contents and changes in the levels of antioxidant enzymes like Guaiacol Peroxidase (GPX, Ascorbate Peroxidase (APX, and Catalase (CAT. Our results showed that cell phone EMR caused significant reduction in growth, fresh weight, dry weight, and relative water contents. Where as MDA contents and antioxidant enzymes were increased in stressed seedlings as compared to unstressed seedlings. We concluded that radiations emitted by mobile phone can induce oxidative stress which results in reduced growth and increase in the activity of antioxidant enzymes in mung bean and wheat seedlings.

  1. Local device parameter extraction of a concentrator photovoltaic cell under solar spot illumination

    Energy Technology Data Exchange (ETDEWEB)

    Munji, M.K.; Okullo, W.; van Dyk, E.E.; Vorster, F.J. [Physics Department, Nelson Mandela Metropolitan University, P O Box 77000, Port Elizabeth 6031 (South Africa)

    2010-12-15

    Focused sunlight can act as a localized source of excess minority carriers in a solar cell. Current signal generated by these carriers gives considerable information about the electrical properties of the cell's material. Point by point current-voltage data were measured for a back point-contact concentrator photovoltaic cell when illuminated by focused sunlight. Two numerical curve fitting procedures: a non-linear two-point interval division and particle swarm optimization algorithm were then applied to extract local parameters (i.e. as function of position) from the current-voltage data at each measurement point. Extracted parameters plotted yields relative spatial information about the electrical properties of a solar cell in a two or three dimensional mapping. The curve fitting routines applied to current-voltage data reveal that performance parameters: short circuit current, open circuit voltage, maximum power and fill factor show distinct variations in the vicinity of the observed current reducing feature. The relative values of the diode ideality factors, series resistance, shunt resistance and reverse saturation currents from both methods showed no significant measurable features that could be distinguished. This shows that the observed reduction in photo-induced current was due to severe recombination in the bulk or around the highly diffused point contacts and not the quality of the multiple p-n junctions of the cell. These approaches allow one to obtain a set of parameters at each local point on the cell which are reasonable and representative of the physical system. (author)

  2. Influence of meteorological parameters on Olea pollen concentrations in Córdoba (south-western Spain).

    Science.gov (United States)

    Vázquez, L M; Galán, C; Domínguez-Vilches, E

    2003-12-01

    The influence of meteorological parameters on the dispersion of airborne pollen has been studied by several authors. Olive pollen is the major cause of allergy in southern Spain, where a large part of the arable surface area is given over to olive cultivation. Daily pollen forecasts provide important information both for pollen-allergy sufferers and for agronomists trying to achieve a better biological understanding of variations in airborne olive pollen levels. The main purpose of this paper is to study, by means of short-term statistical analysis, the effect of meteorological parameters on airborne olive pollen concentrations in the city of Cordoba (south-western Spain). Twenty-one-year (1982-2002) aerobiological and meteorological databases were used. Correlation and multiple regression analyses were used to study the relationships between olive pollen levels and several meteorological parameters. Statistical analysis was applied both to the whole pollen season and to the pre-peak period. Daily meteorological parameters, such as accumulated mean temperature, accumulated sunlight hours, and accumulated rainfall were used as independent variables in both statistical analyses. Accumulated meteorological variables were of the greatest value in most regression analysis equations, heat-related variables being the most important.

  3. The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.

    Science.gov (United States)

    Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

    1991-02-01

    Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis.

  4. The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.

    Science.gov (United States)

    Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

    1991-02-01

    Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis. PMID:2019018

  5. Associations between annual and seasonal variations in body mass and reproductive success and blood biochemical parameters in semi-domesticated reindeer

    Directory of Open Access Journals (Sweden)

    Ingrid H. Holmøy

    2009-12-01

    Full Text Available The main objective of the study was to follow reproductive performance and blood biochemical parameters associated with fat and carbohydrate metabolism in a herd of free-ranging, semi-domesticated reindeer (Rangifer tarandus tarandus over a two-year period, with extreme between-year variation in forage availability. The effects of climatic factors on reindeer reproductive performance were investigated by analysing whether time of onset of luteal function in autumn and calf survival were associated with changes in body mass and weather conditions, such as snow depth, precipitation, and temperature. Considerable between-year variation in the onset of luteal activity was found. In 1997, 4.2% of the female reindeer were either cycling or pregnant in the second week of October, whilst in 1998, in the same week, 100% were cycling or pregnant. Although energy balance was important for timing of the onset of luteal activity, delayed conception had no apparent effect on calf survival. The results indicated that maternal body mass (BM in spring was of primary importance for calf survival, and the productivity of the herd. Since climatic factors influence the availability of forage, and hence female BM, it also has an indirect impact on calf survival. Females with low BM demonstrated greater seasonal variation in BM than heavier females. Plasma concentrations of free fatty acids and β-hydroxy butyric acid responded to changes in forage availability, but the initial condition of the reindeer and their fat reserves also seemed to have a major influence on these parameters.Abstract in Norwegian / Sammendrag:Sammenheng mellom års- og sesongvariasjon i kroppsvekt og henholdsvis reproduksjonssuksess og biokjemiske blodparametre hos tamreinHovedformålet med studien var å følge reproduksjonen og blodparametre knyttet til fett og karbohydratmetabolismen hos en tamreinflokk (Rangifer tarandus tarandus gjennom en toårs periode med stor årlig variasjon i n

  6. Detection of Blood Parameters and Blood Biochemical Parameters in Germ-free Rats%无菌大鼠血液学及血液生化指标正常参考值的测定

    Institute of Scientific and Technical Information of China (English)

    仉慧敏; 孙淑华; 胡晓燕; 王艳蓉; 孟金萍; 刘云波

    2011-01-01

    目的 人工培育Wistar无菌大鼠,观测Fl和F2代无菌大鼠血液学及血液生化指标差异.方法 对清洁级Wistar大鼠进行子宫摘除,隔离器内剥离子宫壁获得大鼠胎仔,人工哺育为无菌大鼠.待其繁殖后,采用全自动血液生化分析仪、全血细胞计数仪测定F1和F2代无菌大鼠的血液学及血液生化指标.结果 不同代数无菌大鼠的血液学和血液生化指标存在显著差异,其中有差异的血液学指标包括RBC、HGB、HCT、MCV、MCHC、RDW、MPV、NE%、LYM%、EO%、BA%、NE#、LYM#、EO#、BA#(P<0.05);有差异的血液生化指标包括ALT、ALB、LDH、CHO、TBIL、HDL-C、LDL-C、GLO、GLU、ALP、A/G、UA、P(P<0.05).同时这些差异又因性别和年龄而不同.结论 代数、性别、年龄对无菌大鼠的血液学及血液生化指标有较大影响.%Objective To determine and compare the blood parameters and blood biochemical parameters in F1 and F2 germ-free rats. Methods Germ-free rats were obtained by uterectomy, and the offspring were fed with artificial milk by hand in the germfree isolator. The blood parameters and blood biochemical parameters were detected by automatic hematology analyzers. Results The germ-free rats showed significant differences in the values of RBC, HGB, HCT, MCV,MCHC, RDW, MPV, NE%, LYM%, EO%, BA%, NE#, LYM #, EO#, BA # ( P < 0.05 ) and ALT, ALB, LDH, CHO, TBIL,H DL-C, LDL-C,GLO,GLU,ALP,A/G, UA,P(P < 0.05 ). There are different blood values in different gender and age.Conclusion The gender and age may affect the blood parameters and blood biochemical parameters.

  7. Determination of acute toxicity and the effects of sub-acute concentrations of CuO nanoparticles on blood parameters in Rutilus rutilus

    Directory of Open Access Journals (Sweden)

    Abdolreza Jahanbakhshi

    2015-07-01

    Full Text Available Objective(s:Copper oxidenanoparticles have different industrial applications so it is inevitable that nanoparticulate products finally find their way into aquatic ecosystems. Nevertheless there is little information available about their effects on some of edible fish. The present study aims to determine the acute toxicity and evaluate the effect of two sub-acute concentrations (50 and 70% 96 h LC50 of CuO-NPs on some hematological and biochemical parameters of R. rutilus. Materials and Methods:225 healthy specimen of R. rutilus (mean weight 5.52±1.2 g; mean length 6.20±0.2 cm were transported to the laboratory. In order to prepare the stock solution, CuO-NPs was dispersed in pure water with ultrasonication (50-60 kHz for 15 min every day before dosing. At first, R. rutilus was exposed to CuO-NPs to determine the lethal concentration (LC50 value. Following acute test, fish were treated with sub-acute concentrations of CuO-NPs (50 and 70% 96 h-LC50 at with one control group (no CuO-NPs for a week to determine the changes in the level of some plasma hematological and biochemical parameters. Results:The 96 h-LC50 values of CuO-NPs was 2.19±0.003 mg/l. R. rutilus exhibited significantly lower RBC count, Hb and Hct values and a significant increase in the WBC numbers, MCH, MCHC and MCV indices (p

  8. Effect of the Telephone-Delivered Nutrition Education on Dietary Intake and Biochemical Parameters in Subjects with Metabolic Syndrome

    OpenAIRE

    Kim, Juyoung; Bea, Wookyung; Lee, Kiheon; Han, Jongsoo; Kim, Sohye; Kim, Misung; Na, Woori; Sohn, Cheongmin

    2013-01-01

    As prevalence of metabolic syndrome has rapidly increased over the past decade, lifestyle changes including dietary habits are considered as a therapeutic cornerstone for metabolic syndrome, cardiovascular complications and type 2 diabetes. We evaluated the effectiveness of a telephone-delivered nutrition education to improve metabolic parameters compared with a single-visit with a dietitian in subjects with metabolic syndrome. A total of seventy-one adults who met diagnostic criteria for the...

  9. Relationship of zinc concentrations in blood and seminal plasma with various semen parameters in infertile subjects

    International Nuclear Information System (INIS)

    To find out relationship of zinc concentrations in blood and seminal plasma with various semen parameters between fertile and infertile men. (JPMC), Karachi and Department of Biochemistry. Basic Medical Sciences Institute, JPMC, Karachi. Fifty eight primary infertile male subjects, without any treatment, who had regular unprotected intercourse for at least 12 months without conception with their partners, aged 20-40 years, were selected from Infertility Clinic Jinnah Postgraduate Medical Center, Karachi. After semen analyses they were grouped as, oligospermic (30), and azoospermic (28). Twenty five known fertile male selected from general population and after semen analysis were taken as normospermic control group. Semen analyzed according to WHO criteria. Serum and seminal plasma zinc were estimated by 5Br. PAPS Colorimetric method. This study showed significant difference in serum and seminal zinc levels in normospermic, oligospermic (p<0.05) and azoospermic (p<0.005). Seminal plasma zinc showed a positive correlation with sperm count and negative with sperm motility in normospermic and oligospermic and negative correlation with volume, pH, WBC concentration in all three groups. There was no correlation found with sperm morphology. On the basis of the findings of this study and those of other reports, zinc may contribute to fertility through its significant effects on various semen parameters. It seems that the estimation of seminal plasma zinc may help in investigation and treatment of infertile males. (author)

  10. Spatial Variation of the Relationship between PM2.5 Concentrations and Meteorological Parameters in China

    Directory of Open Access Journals (Sweden)

    Gang Lin

    2015-01-01

    Full Text Available Epidemiological studies around the world have reported that fine particulate matter (PM2.5 is closely associated with human health. The distribution of PM2.5 concentrations is influenced by multiple geographic and socioeconomic factors. Using a remote-sensing-derived PM2.5 dataset, this paper explores the relationship between PM2.5 concentrations and meteorological parameters and their spatial variance in China for the period 2001–2010. The spatial variations of the relationships between the annual average PM2.5, the annual average precipitation (AAP, and the annual average temperature (AAT were evaluated using the Geographically Weighted Regression (GWR model. The results indicated that PM2.5 had a strong and stable correlation with meteorological parameters. In particular, PM2.5 had a negative correlation with precipitation and a positive correlation with temperature. In addition, the relationship between the variables changed over space, and the strong negative correlation between PM2.5 and the AAP mainly appeared in the warm temperate semihumid region and northern subtropical humid region in 2001 and 2010, with some localized differences. The strong positive correlation between the PM2.5 and the AAT mainly occurred in the mid-temperate semiarid region, the humid, semihumid, and semiarid warm temperate regions, and the northern subtropical humid region in 2001 and 2010.

  11. Association between plasma zinc concentration and zinc kinetic parameters in premenopausal women.

    Science.gov (United States)

    Yokoi, Katsuhiko; Egger, Norman G; Ramanujam, V M Sadagopa; Alcock, Nancy W; Dayal, Hari H; Penland, James G; Sandstead, Harold H

    2003-11-01

    The objective of this study was to measure relationships between plasma zinc (Zn) concentrations and Zn kinetic parameters and to measure relationships of Zn status with taste acuity, food frequency, and hair Zn in humans. The subjects were 33 premenopausal women not taking oral contraceptives and dietary supplements containing iron and Zn. Main outcomes were plasma Zn concentrations, Zn kinetic parameters based on the three-compartment mammillary model using 67Zn as a tracer, electrical taste detection thresholds, and food frequencies. Lower plasma Zn was significantly (P rate constants from the lesser peripheral pool to the central pool and from the central pool to the greater peripheral pool. The break points in the plasma Zn-Zn kinetics relationship were found between 9.94 and 11.5 micromol/l plasma Zn. Smaller size of the lesser peripheral pool was associated with lower frequency of beef consumption and higher frequency of bran breakfast cereal consumption. Hypozincemic women with plasma Zn low Zn status. PMID:12865259

  12. SITE SPECIFIC REFERENCE PERSON PARAMETERS AND DERIVED CONCENTRATION STANDARDS FOR THE SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Jannik, T.

    2013-03-14

    The purpose of this report is twofold. The first is to develop a set of behavioral parameters for a reference person specific for the Savannah River Site (SRS) such that the parameters can be used to determine dose to members of the public in compliance with Department of Energy (DOE) Order 458.1 “Radiation Protection of the Public and the Environment.” A reference person is a hypothetical, gender and age aggregation of human physical and physiological characteristics arrived at by international consensus for the purpose of standardizing radiation dose calculations. DOE O 458.1 states that compliance with the annual dose limit of 100 mrem (1 mSv) to a member of the public may be demonstrated by calculating the dose to the maximally exposed individual (MEI) or to a representative person. Historically, for dose compliance, SRS has used the MEI concept, which uses adult dose coefficients and adult male usage parameters. Beginning with the 2012 annual site environmental report, SRS will be using the representative person concept for dose compliance. The dose to a representative person will be based on 1) the SRS-specific reference person usage parameters at the 95th percentile of appropriate national or regional data, which are documented in this report, 2) the reference person (gender and age averaged) ingestion and inhalation dose coefficients provided in DOE Derived Concentration Technical Standard (DOE-STD-1196-2011), and 3) the external dose coefficients provided in the DC_PAK3 toolbox. The second purpose of this report is to develop SRS-specific derived concentration standards (DCSs) for all applicable food ingestion pathways, ground shine, and water submersion. The DCS is the concentration of a particular radionuclide in water, in air, or on the ground that results in a member of the public receiving 100 mrem (1 mSv) effective dose following continuous exposure for one year. In DOE-STD-1196-2011, DCSs were developed for the ingestion of water, inhalation of

  13. Determination of background concentrations of hydrochemical parameters and water quality assessment in the Akhuryan River Basin (Armenia)

    Science.gov (United States)

    Arpine, Hovhannisyan; Gayane, Shahnazaryan

    2016-08-01

    The determination of background values of hydrochemical parameters, to distinguish between natural concentration and anthropogenically-influenced concentrations, is highly relevant. In presented study, to estimate the background values of hydrochemical parameters in Akhuryan River Basin, log-normal probability functions on the hydrochemical parameters concentrations was applied. The study is carried out on the basis of hydrochemical data of surface water quality monitoring for the period of 2010-2013. This study highlights the usefulness of application of site-specific background concentrations for the evaluation, interpretation of surface water quality and for determination of pollution sources.

  14. Biochemical parameters of Spodoptera frugiperda (J. E. Smith) treated with citronella oil (Cymbopogon winterianus Jowitt ex Bor) and its influence on reproduction.

    Science.gov (United States)

    Silva, Cristiane Thalita Dos Santos; Wanderley-Teixeira, Valéria; Cunha, Franklin Magliano da; Oliveira, José Vargas de; Dutra, Kamilla de Andrade; Navarro, Daniela Maria do Amaral Ferraz; Teixeira, Álvaro Aguiar Coelho

    2016-05-01

    Spodoptera frugiperda is the principal corn pest in Brazil. Searches for new control methods that minimize the adverse effects of synthetic insecticides have initiated a resurgence of the use of botanical insecticides. Citronella oil (a product of Cymbopogon winterianus) is an effective repellent and insecticide. Thus, biochemical profile changes in oil-treated larvae and its influence on reproduction were assessed. Corn leaves dipped in a 50mg/mL concentration were offered to third instar larvae for 24h and assessed in sixth instar to estimate protein, lipid, sugar, and glycogen levels. Adult testes and ovarioles were collected for histological and histochemical analysis 24h after emergence. Number of eggs and hatching rate were also measured. Oil-treated larvae showed an increase in glycogen and a decrease in protein, lipid, and totals sugar content. Control testes exhibited connective tissue lining and cysts with abundant spermatozoids. However, intense peripheral vacuolation and neutral carbohydrates reduction occurred in oil-treated individuals. Control ovarioles showed normal morphologic characteristics. On the other hand, oil-treatment ovarioles showed follicular cell stratification and removal, reduced nurse cell development, reduced yolk quantity, a thinner conjunctiva sheath, and a reduction in proteins and neutral carbohydrates. Eggs derived from oil-treated pairs were unviable. Therefore, sub-lethal doses of citronella oil alters the biochemical profile of S. frugiperda larvae, causing damage to their reproductive histophysiology and results in diminished reproduction or reproductive failure.

  15. [Effect of dietotherapy with food-stuffs for enteral nutrition on the dynamic of clinical and biochemic parameters in biliary tract diseases].

    Science.gov (United States)

    Plotnikova, O A; Meshcheriakova, V A; Sharfetdinov, Kh Kh; Petrovskaia, O B; Mokhova, E O

    2005-01-01

    It was investigated the influence of dietary therapy with enteral formula "Nutricomp AND Braum fiber" on clinical and biochemic parameters in biliary tract diseases. Also it was carried out the comparative estimation of the gallbladder motility in this contingent of patients in process of the standard food loading and the loading with the tested enteral formula (25 g carbohydrates). It was indicated that the including of enteral formula "Nutricomp AND Braum fiber" (209 g/day) in traditional hypocaloric diet promotes the decrease of cholesterol level and activity of alkaline phosphatase. The gallbladder motility was some smaller after the consuming of enteral formula "Nutricomp AND Braum Diabetes" compared with the standard food loading (25 g carbohydrates). PMID:16313133

  16. Evaluation of changes in blood hematological and biochemical parameters in response to Islamic fasting and regular physical activity in male and female subjects

    Directory of Open Access Journals (Sweden)

    Seyyed Reza Attarzadeh Hosseini

    2015-09-01

    Full Text Available Introduction: Fasting during Ramadan is a religious obligation for healthy adult Muslims. This study aimed to investigate the effects of Ramadan fasting and physical activity on blood hematological and biochemical parameters in male and female subjects. Methods: In this study, 50 healthy subjects were randomly assigned to four groups: male control fasting (MCF group (n=13, male training fasting (MTF group (n=13, female control fasting (FCF group (n=12, and female training fasting (FTF group (n=12, and were compared in two stages (before and after the intervention. For inter- and intra-group comparisons, a repeated measure ANOVA was applied. Results: The results showed a significant decline in body weight, body mass index, body fat percentage, and waist-to-hip ratio after Ramadan fasting, compared to the pre-Ramadan period. Also, significant changes were observed in red blood cell count and hematocrit level (P

  17. Salivary biochemical markers as potential acute toxicity parameters for acute radiation injury: A study on small experimental animals.

    Science.gov (United States)

    Soni, S; Agrawal, P; Kumar, N; Mittal, G; Nishad, D K; Chaudhury, N K; Bhatnagar, A; Basu, M; Chhillar, N

    2016-03-01

    Researchers have been evaluating several biodosimetric/screening approaches to assess acute radiation injury, related to mass causality. Keeping in mind this background, we hypothesized that effect of whole-body irradiation in single fraction in graded doses can affect the secretion of various salivary components that could be used as acute radiation injury/toxicity marker, which can be used in screening of large population at the time of nuclear accidents/disaster. Thirty Sprague Dawley rats treated with whole-body cobalt-60 gamma irradiation of dose 1-5 Gy (dose rate: 0.95 Gy/min) were included in this study. Whole mixed saliva was collected from all animals before and after radiation up to 72 h postradiation. Saliva was analyzed for electrolytes, total protein, urea, and amylase. Intragroup comparison of salivary parameters at different radiation doses showed significant differences. Potassium was significantly increased as the dose increased from 1 Gy to 5 Gy (p 0.5). Sodium was significantly altered after 3-5 Gy (p 0.5), except 1 and 2 Gy, whereas changes in sodium level were nonsignificant (p > 0.5). Urea, total protein, and amylase levels were also significantly increased as the radiation dose increased (p 0.5). This study suggests that salivary parameters were sensitive toward radiation even at low radiation dose which can be used as a predictor of radiation injury. PMID:25813962

  18. Effects of dietary supplementation of fermented Ginkgo biloba L. residues on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets.

    Science.gov (United States)

    Zhou, Hao; Wang, Chengzhang; Ye, Jianzhong; Chen, Hongxia; Tao, Ran

    2015-08-01

    This study evaluated the effects of fermented Ginkgo biloba L. residues (FGBLR) on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets. Pigs were allotted to five dietary treatments, including negative control (NC: antibiotic free basal diet), positive control (PC) (NC + 30 mg apramycin/kg) and FGBLR-50, 100, 150 (NC + 50, 100, 150 g FGBLR/kg). Pigs in FGBLR-100 and PC treatments showed increased final body weight, average daily gain, gain:feed and apparent total tract digestibility of dry matter, N and gross energy (P < 0.05) compared with NC, FGBLR-50 and FGBLR-150 treatments, In addition, pigs fed with FGBLR-100 diet showed higher serum total protein, albumin, alkaline phosphatase, glucose, hemoglobin, total iron, total iron binding capacity, superoxide dismutase and glutathione superoxide dismutase levels, and lower serum blood urea nitrogen, malondialdehyde, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, triglyceride and total cholesterol levels than those fed with PC and NC diets (P < 0.05). Moreover, feeding FGBLR-100 could increase levels of immunoglobulin G (IgG), IgA and IgM, as well as lymphocyte transformation rates, ratio of CD4+ to CD8+ cells and proportions of CD2+, CD4+, B, major histocompatibility complex (MHC)-I and MHC-II cells, and can decrease proportion of CD8+ cells in blood of piglets compared with PC and NC groups (P < 0.05). These results indicate that dietary supplementation with 10% of FGBLR showed greatest beneficial effects on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets, which were superior to antibiotic supplemental diets.

  19. INFLUENCE OF SURFACE-ACTIVE RHAMNOLIPID BIOCOMPLEX AND ETHYLTHIOSULFANILATE ON GROWTH AND BIOCHEMICAL PARAMETERS OF PLANTS IN THE OIL CONTAMINATED SOIL

    Directory of Open Access Journals (Sweden)

    A. R. Banya

    2015-10-01

    Full Text Available The aim of the work was to study the influence of rhamnolipid biocomplex and ethylthiosulfanilate on field pea and sorghum plants when growing in petroleum contaminated soil. Plant seeds were treated with solutions of rhamnolipid biocomplex or ethylthiosulfanilate (0.01 g/l before planting and grown in containers with soil artificially contaminated with petroleum (5, 8 and 10%. Effect of rhamnolipid biocomplex and ethylthiosulfanilate was determined by the determination of growth (weight, length plants and biochemical parameters (content of photosynthetic pigments, hydrogen peroxide, malondialdehyde. The stimulating effect of rhamnolipid biocomplex and ethylthiosulfanilate on growth parameters of field pea was shown: the shoot mass has significantly increased on 39%, root mass – on 26% if compared with the control. For sorghum somewhat smaller increase of growth parameters was observed. Under the influence of rhamnolipid biocomplex and ethylthiosulfanilate the content of photosynthetic pigments in field pea and sorghum has also increased. It was determined that the action rhamnolipid biocomplex and ethylthiosulfanilate promoted the decrease of indicators of plant oxidative reactions if compared with control: the content of hydrogen peroxide – in average on 15% and 16%, malondialdehyde – on 13.5% and 16% respectively. The results of the study testify to the effectiveness of rhamnolipid biocomplex and ethylthiosulfanilate as growth stimulators for field pea and sorghum, as well as improvement of adaptive capability of plants to unfavorable conditions. It creates the prospects of their application as effective and ecologically safe substances for the intensification of contaminated soil phytoremediation.

  20. The Metabolic Effects of Consumption of Yellow Cassava (Manihot esculenta Crantz) on Some Biochemical Parameters in Experimental Rats.

    Science.gov (United States)

    Udeme, Nelson; Okafor, Polycarp; Eleazu, Chinedum

    2015-01-01

    The metabolism of yellow cassava (variety TMS 01/1368) was investigated in male albino rats fed a diet containing yellow cassava for 7 to 28 days. There were significant increases (P 0.05) in the serum total proteins of the experimental rats compared with the control. The experimental rats treated for 7, 14, 21, or 28 days exhibited body weight decreases of 5.11%, 11.10%, 19.16%, and 24.18%, respectively, whereas the control group showed 9.17% gain in body weight. Total and free cyanide concentrations were detected in the liver, kidney, and heart of most of the rats in both the experimental and control groups, except for free cyanide in the control group that was not detected. Metabolism of the yellow cassava variety in experimental rats was capable of exposing the animals to cyanide, underscoring the need for its proper processing before consumption by humans.

  1. DNA Duplex Length and Salt Concentration Dependence of Enthalpy−Entropy Compensation Parameters for DNA Melting

    KAUST Repository

    Starikov, E. B.

    2009-08-20

    Systematical differential calorimetry experiments on DNA oligomers with different lengths and placed in water solutions with various added salt concentrations may, in principle, unravel important information about the structure and dynamics of the DNA and their water-counterion surrounding. With this in mind, to reinterpret the most recent results of calorimetric experiments on DNA oligomers of such a kind, the recent enthalpy-entropy compensation theory has been used. It is demonstrated that the application of the latter could enable direct estimation of thermodynamic parameters of the microphase transitions connected to the changes in DNA dynamical regimes versus the length of the biopolymers and the ionic strengths of their water solutions, and this calls for much more systematical experimental and theoretical studies in this field. © 2009 American Chemical Society.

  2. DNA duplex length and salt concentration dependence of enthalpy-entropy compensation parameters for DNA melting.

    Science.gov (United States)

    Starikov, E B; Nordén, Bengt

    2009-08-20

    Systematical differential calorimetry experiments on DNA oligomers with different lengths and placed in water solutions with various added salt concentrations may, in principle, unravel important information about the structure and dynamics of the DNA and their water-counterion surrounding. With this in mind, to reinterpret the most recent results of calorimetric experiments on DNA oligomers of such a kind, the recent enthalpy-entropy compensation theory has been used. It is demonstrated that the application of the latter could enable direct estimation of thermodynamic parameters of the microphase transitions connected to the changes in DNA dynamical regimes versus the length of the biopolymers and the ionic strengths of their water solutions, and this calls for much more systematical experimental and theoretical studies in this field. PMID:19719257

  3. Zearalenone-induced changes in biochemical parameters, oxidative stress and apoptosis in cardiac tissue: Protective role of crocin.

    Science.gov (United States)

    Salem, I Ben; Boussabbeh, M; Neffati, F; Najjar, M F; Abid-Essefi, S; Bacha, H

    2016-06-01

    Zearalenone (ZEN) is a mycotoxin from Fusarium species commonly found in food commodities and is known to cause reproductive disorders. Several in vivo studies have shown that ZEN is haematotoxic and hepatotoxic and causes several alterations of immunological parameters. Meantime, the available information on the cardiotoxic effects of ZEN is very much limited. In the present study, we investigated the toxic effects of ZEN in heart tissues of Balb/c mice. We demonstrated that ZEN (40 mg kg(-1) body weight (b.w.)) increased creatine phosphokinase, lactate dehydrogenase, aspartate transaminase, alanine transaminase, total cholesterol and triglyceride levels and induced oxidative stress as monitored by measuring the malondialdehyde level, the generation of protein carbonyls, the catalase and superoxide dismutase activity and the expression of the heat shock proteins (Hsp 70). We also demonstrated that acute administration of ZEN triggers apoptosis in cardiac tissue. Furthermore, we aimed to evaluate the safety and efficacy of crocin (CRO), a natural carotenoid, to prevent ZEN-induced cardiotoxicity in mice. In fact, combined treatment of ZEN with different doses of CRO (50, 100, and 250 mg kg(-1) b.w.) showed a significant reduction of ZEN-induced toxicity for all tested markers in a dose-dependent manner. It could be concluded that CRO was effective in the protection against ZEN-induced toxicity in cardiac tissue. PMID:26231423

  4. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Al-Okbi, S. Y.; Mohamed, D. A.; Donya, S. M.; Abd El Khalek, A. B.

    2011-07-01

    The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum) were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, {beta}-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory bio markers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures. (Author) 61 refs.

  5. Effects of nickel supply on the fattening performance and several biochemical parameters of broiler chickens and rabbits.

    Science.gov (United States)

    Bersényi, A; Fekete, S Gy; Szilágyi, M; Berta, Erzsébet; Zöldág, L; Glávits, R

    2004-01-01

    Broiler chicken and rabbit experiments were carried out to study the effects of nickel (Ni) supplementation on growth performance and Ni metabolism. ROSS cockerels and New Zealand White female rabbits were fed a diet containing Ni in concentrations of 0, 50 and 500 mg/kg in dry matter (DM). Dietary supplementation of 50 mg Ni/kg slightly improved the body weight gain (BWG) and had a beneficial effect on the feed conversion efficiency (FCE) in broiler chickens. However, Ni added at a level of 500 mg/kg significantly (P supplementation of 500 mg Ni/kg, indicating damage of the liver parenchyma. The results of serum biochemistry were confirmed by a mild or moderate form of pathological focal fatty infiltration of the liver in broilers. Supplemental Ni of 50 mg/kg concentration resulted in non-significantly increased BWG in rabbits. Ni added to the diet at a level of 500 mg/kg reduced the digestibility of crude protein by 3-4% and that of crude fibre by 20-25% in rabbits. Approx. 98% of the ingested Ni was lost from the body via the faeces, 0.5-1.5% via the urine and approx. 1% was incorporated into the organs of rabbits. As a result of dietary supplementation of 50 and 500 mg Ni/kg, Ni accumulated in the kidneys (4.9 +/- 0.5 and 17.1 +/- 3.1 vs. 1.9 +/- 0.3 mg/kg DM), ribs (10.3 +/- 0.4 and 10.4 +/- 0.6 vs. 9.1 +/- 0.6 mg/kg DM), heart (1.4 +/- 0.2 and 2.5 +/- 0.4 vs. 1.0 +/- 0.1 mg/kg DM) and liver (1.3 +/- 0.1 and 2.2 +/- 0.2 vs. 0.9 +/- 0.05 mg/kg DM), as compared to the control animals. It can be stated that supplementation of the diet with 50 mg Ni/kg had slight but non-significant beneficial effects on the growth performance of broiler chickens and rabbits.

  6. The Metabolic Effects of Consumption of Yellow Cassava (Manihot esculenta Crantz) on Some Biochemical Parameters in Experimental Rats.

    Science.gov (United States)

    Udeme, Nelson; Okafor, Polycarp; Eleazu, Chinedum

    2015-01-01

    The metabolism of yellow cassava (variety TMS 01/1368) was investigated in male albino rats fed a diet containing yellow cassava for 7 to 28 days. There were significant increases (P cyanide and thiocyanate in the sera and urine samples of the experimental rats compared with the control, significant increases (P 0.05) in the serum total proteins of the experimental rats compared with the control. The experimental rats treated for 7, 14, 21, or 28 days exhibited body weight decreases of 5.11%, 11.10%, 19.16%, and 24.18%, respectively, whereas the control group showed 9.17% gain in body weight. Total and free cyanide concentrations were detected in the liver, kidney, and heart of most of the rats in both the experimental and control groups, except for free cyanide in the control group that was not detected. Metabolism of the yellow cassava variety in experimental rats was capable of exposing the animals to cyanide, underscoring the need for its proper processing before consumption by humans. PMID:26467190

  7. Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio).

    Science.gov (United States)

    Dong, Xiao-Qing; Zhang, Dong-Ming; Chen, Yu-Ke; Wang, Qiu-Ju; Yang, Yi-Yu

    2015-11-01

    Antibiotic use in livestock feed additives has resulted in harmful residue accumulation and spread of drug-resistance. We examined the use of antimicrobial peptides (AMPs) as a safer alternative to antibiotics in feeding the common carp. AMPs were added to common carp basal diets (Control) as additives at four concentrations: 100 mg kg(-1) (B1), 200 mg kg(-1) (B2), 400 mg kg(-1) (B3), 600 mg kg(-1) (B4) by dry weight of basal diet. After a 60-day feeding experiment, the final weight, DG and SGR of carps on B1, B2 and B3 diet were significantly higher than the control (p diet were significantly lower than the control (p diets showed significantly lower (p 0.05) in levels of uric ammonia, globulin, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, lactic dehydrogenase and blood glucose in all groups. The serum superoxide dismutase and catalase activity of B1-fed carps was significantly higher (p alkaline phosphate activity of carps on B1 diets was significantly higher (p diets was significantly higher (p diets for common carp increased the final weight, DG, SGR and decreased the FCR. PMID:26386195

  8. Quantifying biochemical quality parameters in carrots (Daucus carota L.) - FT-Raman spectroscopy as efficient tool for rapid metabolite profiling.

    Science.gov (United States)

    Krähmer, Andrea; Böttcher, Christoph; Rode, Andrea; Nothnagel, Thomas; Schulz, Hartwig

    2016-12-01

    Application of FT-Raman spectroscopy for simultaneous quantification of carotenoids, carbohydrates, polyacetylenes and phenylpropanoids with high bioactive potential was investigated in storage roots of Daucus carota. Within single FT-Raman experiment carbohydrates, carotenoids, and polyacetylenes could be reliably quantified with high coefficients of determination of R(2)>0.91. The most abundant individual representatives of each compound class could be quantified with comparably high quality resulting in R(2)=0.97 and 0.96 for α-carotene and β-carotene, in R(2)=0.90 for falcarindiol (FaDOH), R(2)=0.99, 0.98 and 0.96 for fructose, glucose and sucrose. In contrast, application of FT-Raman spectroscopy for quantification of two laserine-type phenylpropanoids was investigated but failed due to low concentration and Raman response. Furthermore, evaluation of metabolic profiles by principle component analysis (PCA) revealed metabolic variety of carrot root composition depending on root color and botanical relationship. PMID:27374560

  9. Soil air CO2 concentration as an integrative parameter of soil structure

    Science.gov (United States)

    Ebeling, Corinna; Gaertig, Thorsten; Fründ, Heinz-Christian

    2015-04-01

    The assessment of soil structure is an important but difficult issue and normally takes place in the laboratory. Typical parameters are soil bulk density, porosity, water or air conductivity or gas diffusivity. All methods are time-consuming. The integrative parameter soil air CO2 concentration ([CO2]) can be used to assess soil structure in situ and in a short time. Several studies highlighted that independent of soil respiration, [CO2] in the soil air increases with decreasing soil aeration. Therefore, [CO2] is a useful indicator of soil aeration. Embedded in the German research project RÜWOLA, which focus on soil protection at forest sites, we investigated soil compaction and recovery of soil structure after harvesting. Therefore, we measured soil air CO2 concentrations continuously and in single measurements and compared the results with the measurements of bulk density, porosity and gas diffusivity. Two test areas were investigated: At test area 1 with high natural regeneration potential (clay content approx. 25 % and soil-pH between 5 and 7), solid-state CO2-sensors using NDIR technology were installed in the wheel track of different aged skidding tracks in 5 and 10 cm soil depths. At area 2 (acidic silty loam, soil-pH between 3.5 and 4), CO2-sensors and water-tension sensors (WatermarkR) were installed in 6 cm soil depth. The results show a low variance of [CO2] in the undisturbed soil with a long term mean from May to June 2014 between 0.2 and 0.5 % [CO2] in both areas. In the wheel tracks [CO2] was consistently higher. The long term mean [CO2] in the 8-year-old-wheel track in test area 1 is 5 times higher than in the reference soil and shows a high variation (mean=2.0 %). The 18-year-old wheel track shows a long-term mean of 1.2 % [CO2]. Furthermore, there were strong fluctuations of [CO2] in the wheel tracks corresponding to precipitation and humidity. Similar results were yielded with single measurements during the vegetation period using a portable

  10. Postmortem biochemical behavior of giant squid (Dosidicus gigas) mantle muscle stored in ice and its relation with quality parameters.

    Science.gov (United States)

    Márquez-Ríos, E; Morán-Palacio, E F; Lugo-Sánchez, M E; Ocano-Higuera, V M; Pacheco-Aguilar, R

    2007-09-01

    Several freshness and spoilage indicators were monitored to characterize the postmortem biochemistry of giant squid (Dosidicus gigas) mantle muscle. Squid samples were obtained directly from the sea and kept at 0 degrees C during a 15-d storage period. Data at zero time were obtained from cryogenically frozen samples at time of capture. The adenosine 5'-triphosphate (ATP) degradation followed a different pattern as compared with that from fish species. ATP was almost completely depleted at 24-h postcatch from 6.54 to <1 micromol/g, while at the same time Hx was the predominant catabolite with a concentration of 4 mumol/g, reaching 6.85 micromol/g at day 15. K-value data followed a logarithmic pattern with time instead of a linear one, with no change after day 3, thus reducing its suitability as a freshness index. The coefficient Hx/AMP seems to be an adequate alternative for this purpose due to its constant increment with time. The high NH4Cl content in mantle muscle (461.3 +/- 24.5 mg of NH4(+)/100 g) derived from its physiological importance for the species compromises the use of the distillation step of the TVB-N analysis commonly used as a spoilage index. This fact explains why the initially high value of TVB-N detected in mantle muscle (243.7 mg N/100 g) did not correlate with the initial low TMA-N content (1.5 +/- 0.1 mg/100 g of muscle). The results suggested that under the experimental conditions the shelf life of squid exceeds 15 d. PMID:17995632

  11. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

    Directory of Open Access Journals (Sweden)

    Donya, S. M.

    2011-09-01

    Full Text Available The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, β-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory biomarkers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures.El objetivo de la presente investigación fue encontrar extractos de alimentos vegetales y probióticos que puedan tener bioactividad hacía la inflamación crónica. Mezclas de tres extractos de alimentos vegetales conocidos por su riqueza en compuestos fenólicos, carotenoides y tocoferoles han sido preparadas. La actividad anti-inflamatoria de las diferentes mezclas y de bacterias probióticas (Bifidobacterium bifidum fue evaluada en artritis adyuvante en ratas. El efecto anti-inflamatorio, mecanismo de acción y salubridad de las tres mezclas y de Bifidobacterium bifidum ha sido estudiado mediante la medida del tamaño de la inflamación y la determinación de biomarcadores de inflamación y estrés oxidativo, del perfil de bacterias del colón y de parámetros citogen

  12. EFFECT OF FEEDING AMARANTH (AMARANTHUS ON AQUACULTURAL AND SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF AGE-2 CARP

    Directory of Open Access Journals (Sweden)

    R. Palamarchuk

    2016-06-01

    with artificial feeds of improved composition. This will allow reducing the cost of artificial feeds, obtaining higher growth rate, and improving physiological parameters of cultured carp.

  13. Relationship between dietary intake and use of protease inhibitors with anthropometric and biochemical parameters of lipodystrophy in people living with hiv.

    Science.gov (United States)

    da Silva, Tatiane Andreza Lima; Barboza, Renata Rangel; de Andrade, Ricardo Dias; de Medeiros, Rafaela Catherine da Silva Cunha; de Medeiros, Jason Azevedo; de Souza, Hunaway Albuquerque Galvão; Leite, Lúcia Dantas; Dantas, Paulo Moreira Silva

    2014-10-01

    The lipodystrophy syndrome is characterized by redistribution of body fat and disorders of glicidic and lipid metabolism. Although its etiology is related to infection and drug therapy, there is little evidence regarding the nutritional disturbances on this association. This study aimed to assess the relationship between dietary intake and use of protease inhibitors (PIs) with anthropometric and biochemical parameters in HIV positive patients. The study included 50 patients. A questionnaire about socioeconomic status, lifestyle and infection history was taken. In addition, it was conducted the evaluation of dietary intake (frequency questionnaire), anthropometric parameters (body mass index, waist circumference, triceps skinfold, corrected arm muscle area) and biochemistry tests (glycemia and lipid profile). Only 37% of the sample was classified as "good food consumption", 54% were overweight or obese and 66% presented high waist circumference. The group with good food consumption had higher HDL-C (p=0.04) levels than the group with poor food consumption. Patients taking PIs presented VLDL-C (p=0.023) and triglycerides (p=0.024) levels significantly higher. These results indicated the necessity for continuous monitoring of HIV-positive patients and non-pharmacological interventions such as nutrition education and practice of physical exercises.

  14. Growing Chlorella vulgaris in Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

    Directory of Open Access Journals (Sweden)

    Ângelo Paggi Matos

    2014-01-01

    Full Text Available Desalination wastewater, which contains large amount of salt waste, might lead to severely environmental pollution. This study evaluated the effect of dilution rate (0.1≤D≤0.3 day−1 on microalgal biomass productivity, lipid content, and fatty acid profile under steady-state condition of Chlorella vulgaris supplemented with concentrated desalination. Continuous culture was conducted for 55 days. Results show that the biomass productivity (Px varied from 57 to 126 mg L−1 d−1 (dry mass when the dilution rate ranged from 0.1 to 0.3 day−1. At lowest dilution rate (D=0.1 day−1, the continuous culture regime ensured the highest values of maximum biomass concentration (Xm=570±20 mL−1 and protein content (52%. Biomass lipid content was an increasing function of D. The most abundant fatty acids were the palmitic (25.3±0.6% at D=0.1 day−1 and the gamma-linolenic acid (23.5±0.1% at D=0.3 day−1 ones. These fatty acids present 14 to 18 carbons in the carbon chain, being mainly saturated and polyunsaturated, respectively. Overall, the results show that continuous culture is a powerful tool to investigate the cell growth kinetics and physiological behaviors of the algae growing on desalination wastewater.

  15. Monitoring the effects of exposure to lead and cadmium in working and living environment through standard biochemical blood parameters and liver endonucleases activity

    Directory of Open Access Journals (Sweden)

    Nikolić Ružica S.

    2011-01-01

    Full Text Available Heavy metals as pollutants in the working and living environment are a serious health and environmental problem because they are toxic, non-biodegradable, accumulate in living systems and have a long half-life in soil. Sources of lead contamination are combustion products in the chemical industry and metallurgy, industrial waste water, landfills, traffic etc. Lead enters into the body via the food chain and drinking water. In the body lead is deposited in the liver, kidneys, brain and mineral tissues. Excretion of lead causes damage to the epithelial cells of certain organs. High level exposure to cadmium is usually the result of environmental pollution by human activities. Exposure to cadmium can lead to acute and chronic tissue damage of various organs, including liver and kidneys in humans and in animals. In this paper we analyzed the effects of lead and cadmium exposure, in working and living environment, on the model system of experimental animals, particularly the activity of certain liver enzymes, acid and alkaline DNase, and standard biochemical blood parameters. The study showed that lead and cadmium significantly affect the protein content, red blood cells, hemoglobin and hematocrit, and the activity of liver enzymes. This harmful effect of this toxic metal can be reduced by the supplements.

  16. Effects of aloe arborescens ingestion on azoxymethane-induced intestinal carcinogenesis and hematological and biochemical parameters of male F344 rats.

    Science.gov (United States)

    Shimpo, Kan; Beppu, Hidehiko; Chihara, Takeshi; Kaneko, Takaaki; Shinzato, Masanori; Sonoda, Shigeru

    2006-01-01

    We examined the modifying effect of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Kidachi aloe in Japan; designated as 'ALOE') on azoxymethane (AOM)-induced intestinal carcinogenesis in rats. Male F344 rats (4 weeks old) were fed basal diet or experimental diet containing 0.2% or 1% ALOE for 28 weeks. Starting two weeks later, the animals received subcutaneous injections of AOM once weekly for 10 weeks. The incidence of colorectal adenocarcinomas in the 0.2% (but not 1%) ALOE group showed a strong tendency for decrease (p = 0.056) from the control group. Further, the adenocarcinoma incidence in the entire intestine (small and large intestines) in the 0.2% ALOE group was significantly (p = 0.024) decreased compared to the control value. However, there were no significant differences in tumor multiplicities of colorectal or entire intestines among the 3 groups. In addition, we also studied the safety of long-term ingestion of ALOE as a health food or natural thickening stabilizer. Rats were fed the basal diet or 1% ALOE diet for 35 weeks without AOM treatment. Feeding with 1% ALOE did not affect most hematological and serum biochemical parameters in the rats. These results indicate that a low level of ALOE ingestion might have a mild suppressive effect on intestinal tumor growth without harmful side effects. PMID:17250432

  17. Blood haematological and biochemical parameters in normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) maintained in isothermic and isonutritional conditions

    Institute of Scientific and Technical Information of China (English)

    Anthony Sabasthin; Venkataswamy Girish Kumar; Sumanta Nandi

    2012-01-01

    Objective:The present study was envisaged to examine the hematological and biochemical, parameters in three different groups of buffaloes (regularly cycling, pregnant and repeat breeding) maintained in isothermic and isonutritional conditions to establish the variations in blood and/or serum components in these groups.Methods:Blood samples were analyzed for hemoglobin, PCV, TLC, neutophil, lymphocyte, eosinophil, and monocyte count, glucose, total protein, albumin, globulin, urea and cholesterol.Results:The results revealed significantly lower haemoglobin and packed cell volume value in repeat breeding when compared to pregnant and regularly cycling animals. The WBC value significantly higher in repeat breeding when compared to regularly cycling animals. The average mean values of neutrophils, lymphocyte and eosinophils revealed a no significant difference in the neutrophils, lymphocyte and eosinophils in between the group. The mean values of monocytes revealed a significantly higher value in repeat breeding animals when compared to pregnant animals. The serum glucose, total protein, cholesterol and urea levels were significantly lower in repeat breeding compared to pregnant and regularly cycling animals. The levels of albumin and globulin revealed non-significant difference among the groups.Conclusions: A significant decrease in the hemoglobin, PCV, glucose, total protein, cholesterol and urea was observed in the repeat breeding animals when compared to pregnant and regularly cycling animals.

  18. Effect of acute exposure to nonylphenol on biochemical, hormonal, and hematological parameters and muscle tissues residues of Nile tilapia; Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Hager Tarek H. Ismail

    2016-06-01

    Full Text Available Aim: This study is aimed to evaluate some biochemical, hormonal, hematological, and histopathological changes in Nile tilapia, Oreochromis niloticus, after acute exposure to nonylphenol (NP. In addition to detection of NP residues in the fish, muscle tissues for human health concern. Materials and Methods: A total of 90 apparently healthy Nile tilapia, O. niloticus, were randomly divided into three equal groups; each containing 30 fish (three replicates. Groups 1 and 2 kept as a control and solvent control (acetone, respectively, and Group 3 exposed to NP at a dose level of 500 μg/L water for 7 successive days. Blood and tissue samples were collected 2 times randomly from each group after 7 days from fish exposure to NP and 10 days from exposure stopping. Results: Fish exposed to NP Group 3 showed anorexia, sluggish movement, erythema of the skin, areas of scales loss, and hemorrhagic ulcers in some areas of body region leading to exposing the viscera. Biochemical results revealed a significant increase in serum total proteins and globulins levels, a highly significant increase in serum alanine aminotransferase and aspartate aminotransferase activities, triglycerides, cholesterol, and creatinine levels, insignificant increase in serum uric acid level, and a highly significant decrease in serum testosterone and estradiol-β17 levels in Group 3 in compare with the control group. Histopathological finding confirms these results. While hematological results of the same group revealed a significant increase in red blood cells count and packed cell volume value, insignificant increase in hemoglobin concentration, leukopenia, lymphopenia, and monocytopenia in compared with the control group. All of these changes appeared after 7 days from fish exposure to NP. Most of these alterations returned toward the normal level after 10 days from stopping exposure to NP. NP residues detected in fish muscle tissues of Group 3 during exposure and after stopping

  19. Mercury concentrations in human placenta, umbilical cord, cord blood and amniotic fluid and their relations with body parameters of newborns

    International Nuclear Information System (INIS)

    Studies were conducted on samples taken from giving birth women (n = 40) living in Poland, representing three age groups: 19–25, 26–30 and 31–38 years old. Mercury concentrations were measured with CV-AAS in placenta, umbilical cord, cord blood and amniotic fluid. The placentas weight did not exceed the 750 g value and was heavier than 310 g. Mean values of Hg concentrations in blood, placenta and umbilical cord were similar (c.a. 9 μg/g). High levels of mercury were noted in cord blood which in 75% of all observations exceeded (up to 17 μg/L) the safe dose set by US EPA (5.8 μg/L). No statistically significant differences in medium level of Hg in all the studied tissues among age groups of women were observed. Positive correlations between Hg concentrations in placenta and umbilical cord and cord blood were revealed as well as some negative ones between mercury concentrations and pregnancy parameters. -- Highlights: •Concentrations of mercury in cord blood exceed the safety threshold level. •Maternal age was not an influential factor of Hg concentrations in studied samples. •Positive correlations between Hg levels in different tissues were observed. •Negative correlation between Hg concentrations and pregnancy parameters were noted. -- Maternal age was not an influential factor of mercury concentrations in studied samples. 75% of cord blood samples exceeded the Hg threshold concentration

  20. Indoor air quality in the Karns research houses: baseline measurements and impact of indoor environmental parameters on formaldehyde concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, T. G.; Fung, K. W.; Tromberg, B. J.; Hawthorne, A. R.

    1985-12-01

    Baseline indoor air quality measurements, a nine-month radon study, and an environmental parameters study examining the impact of indoor temperature (T) and relative humidity (RH) levels on formaldehyde (CH2O) concentrations have been performed in three unoccupied research homes located in Karns, Tennessee. Inter-house comparison measurements of (1) CH2O concentration, (2) CH20 emission rates from primary CH20 emission sources, (3) radon and radon daughter concentrations, and (4) air exchange rates indicate that the three homes are similar. The results of the nine-month radon study indicate indoor concentrations consistently below the EPA recommended level of 4 pCi/L. Evidence was found that crawl-space concentrations may be reduced using heat pump systems whose outdoor units circulate fresh air through the crawl-spaoe. The modeled results of the environmental parameters study indicate approximate fourfold increases in CH20 concentrations from 0.07 to 0.27 ppm for seasonal T and RH conditions of 20°C, 30% RH and 29°C, 80% RH, respectively. Evaluation of these environmental parameters study data with steady-state CH2O concentration models developed from laboratory studies of the environmental dependence of CH2O emissions from particleboard underlayment indicate good correlations between the laboratory and field studies.

  1. Predicting the Activity Coefficients of Free-Solvent for Concentrated Globular Protein Solutions Using Independently Determined Physical Parameters

    OpenAIRE

    McBride, Devin W; Rodgers, Victor G. J.

    2013-01-01

    The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent...

  2. Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers

    Institute of Scientific and Technical Information of China (English)

    Hua-li LI; Zong-jun LI; Zhong-shan WEI; Ting LIU; Xiao-zuo ZOU; Yong LIAO; Yu LUO

    2015-01-01

    This study investigates the long-term effects of oral tea polyphenols (TPs) and Lactobacillus brevis M8 (LB) on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1, 240 broiler chickens were selected to investigate the effects of 0.06 g/kg body weight (BW) TP and 1.0 ml/kg BW LB on broilers;in experiment 2, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of TP (0.03, 0.06, and 0.09 g/kg BW) combined with 1.0 ml/kg BW LB on broilers;in experiment 3, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of LB (0.5, 1.0, and 1.5 ml/kg BW) combined with 0.06 g/kg BW TP on broilers. The results showed that TP and LB affected serum bio-chemical parameters, and TP reduced serum cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) abundances in a dosage-dependent manner (P  目的:茶多酚和乳酸菌在动物营养上的作用已经得到广泛的验证,但是关于二者联合作用的研究鲜有报道。本文采用肉鸡作为试验模型,研究了茶多酚和乳酸菌联合灌喂对肉鸡血液生化、消化酶以及肠道细胞因子表达的影响。  创新点:本研究首次采用联合灌喂茶多酚和乳酸菌,探讨了二者联合作用对肉鸡的影响,并通过模拟生产,长期观察了茶多酚和乳酸菌对肉鸡的影响。  方法:对肉仔鸡灌喂不同浓度的茶多酚和乳酸菌,在第56和84天随机屠宰取样。收集血液检查血液生化指标,并测定消化酶活性。取肠道样品提取RNA,采用反转录聚合酶链反应(RT-PCR)检测细胞因子的表达以及相关信号通路的激活。  结论:长期灌喂茶多酚和乳酸菌改善了肉鸡脂质代谢、消化酶活性以及炎症反应,其机制可能是通过影响了NF-κB信号通路。

  3. Does the adequacy parameter Kt/V-urea reflect uremic toxin concentrations in hemodialysis patients?

    OpenAIRE

    Eloot, Sunny; van Biesen, Wim; Glorieux, Griet; Neirynck, Nathalie; Dhondt, Annemieke; Vanholder, Raymond

    2013-01-01

    Hemodialysis aims at removing uremic toxins thus decreasing their concentrations. The present study investigated whether Kt/Vurea, used as marker of dialysis adequacy, is correlated with these concentrations. Predialysis blood samples were taken before a midweek session in 71 chronic HD patients. Samples were analyzed by colorimetry, HPLC, or ELISA for a broad range of uremic solutes. Solute concentrations were divided into four groups according to quartiles of Kt/Vurea, and also of different...

  4. Protective Effects of Vitamin C (Ascorbic Acid in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

    Directory of Open Access Journals (Sweden)

    Alireza AYOUBI

    2015-01-01

    Full Text Available The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42 were randomly assigned into7 groups: I healthy; II fed lead acetate only; III vitamin C administered only; IV diabetic; V diabetic rats administered by vitamin C; VI diabetic rats given lead acetate and VII diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

  5. Influence of Dietary Zinc and Vitamin C Supplementation on Some Blood Biochemical Parameters and Egg Production in Free-Range Laying Hens

    Directory of Open Access Journals (Sweden)

    Vasko GERZILOV

    2015-03-01

    Full Text Available The study aimed to follow out the effect of antistress dietary supplements Zinteral 35 and vitamin C on the levels of some blood biochemical parameters (corticosterone, total cholesterol, glucose, total protein and creatinine and egg production in laying hens during cold (7o C, thermoneutral (19o C and hot (31o C periods. The fowls were divided in three groups (26 females and 3 males in each group. They were reared in a free-range management system with elements of organic production. The experimental treatments were as followed: first (control group without dietary supplement, second group with 100 mg Zinteral 35 per kg diet containing 35 mg/kg zinc oxide, third group with the same amount of Zinteral 35 together with 250 mg vitamin C per kg diet. During the three periods with different ambient temperature, the hens supplemented either with zinc alone (second group or co-administered zinc + vitamin C (third group had significantly lower levels of plasma corticosterone (P<0.001, serum cholesterol (P<0.05 and glucose (P<0.05 than those from the first (control group. The differences between the third and the first groups were bigger versus those between the second and the first groups. For the entire period (March 1 and June 21, egg production was higher by 2.22 % and 4.60 % in the second and third groups respectively in comparison to the first group. The combination of 100 mg Zinteral 35 and 250 mg vitamin C per 1 kg diet exhibited a synergistic effect in reducing cold and heat stress in laying hens and increased their egg production.

  6. Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio by Vitamin C and Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2015-12-01

    Full Text Available The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg/L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg/kg diet, vitamin C (1000 mg/kg diet or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA as well as biochemical parameters, including the activity of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, and acetylcholinesterase (AChE were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium-treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.

  7. Diagnosis and follow-up of idiopathic retroperitoneal fibrosis: role of (18)F-FDG-PET/CT and biochemical parameters in patients with renal involvement.

    Science.gov (United States)

    Fofi, Claudia; Prosperi, Daniela; Pettorini, Laura; Festuccia, Francescaromana; Pirisino, Riccardo; Lanni, Valerio; Scopinaro, Francesco; Punzo, Giorgio; Menè, Paolo

    2016-09-01

    Idiopathic retroperitoneal fibrosis (IRF) is a rare disease characterized by fibro-inflammatory reaction surrounding ureters and other inner organs with possible secondary renal involvement. Symptoms are aspecific and recurrent phases of activity are generally associated with elevation of inflammatory indices. 18F-FDG-PET is nowadays an important tool for the detection of this disease, allowing differentiation between metabolically active tissue and fibrotic one. The purpose of this study was to investigate the role of 18F-FDG-PET in the management of IRF and to evaluate possible correlations between biochemical parameters and PET/CT findings of disease activity. We enrolled seven consecutive patients with IRF (in five histology proved the disease) observed from 2003 to 2012 (5 M:2 F, mean age 53.8 years, range 44-86 years). All patients presented with fever as first symptom; two had obstructive renal failure requiring hemodialysis; one underwent monolateral nephrectomy for parenchyma infiltration; six presented ureteral involvement; three underwent ureteral stent placement. For each patient, during a mean total follow-up of 26.5 months we evaluated serum creatinine, BUN, Hb, RBCs, WBCs, PLT, CRP, ESR. Periodic 18F-FDG-PET/CT scans (every 5.9 months-mean) were performed in all patients. Statistical evaluation was performed using "stepwise regression" analysis. Steroids and immunosuppressive agents induced a progressive normalization of PET/CT scans in all patients at the end of follow-up. Stepwise regression analysis showed that BUN, serum creatinine and CRP only if considered together, significantly correlated with SUV max (p value = 0.000003057). 18F-FDG-PET is a useful tool for clinical decision making in patient with IRF, allowing to evaluate the efficacy of the pharmacological treatment and to detect early recurrences, to modify the therapeutic approach. Acute phase reactants are not reliable alone for the management and the follow-up as they are often

  8. The Effect of Cadmium on Physiological Indices, Growth Parameters and Nutrient Concentration in Tomato in Soilless Culture

    Directory of Open Access Journals (Sweden)

    Z. Ghasemi

    2010-08-01

    Full Text Available In order to determine the effects of cadmium on growth, physiological parameters, and nutritional elements’ concentration in tomato organs in a soilless system, an experiment was conducted in greenhouse of Isfahan Agricultural and Natural Resources Research Center in 2008 with a complete randomized block design with two treatments and 6 replications. The treatments were nutritional solutions of Hoagland with and without cadmium (20 µM. The measured indices included: RGR, NAR, RLGR, LAR, SLA, LWR, LWCA, length of the stem, fresh and dry weight of root and shoot, amount of sugar, photosynthetic pigments, and concentration of nitrogen, potassium, phosphorous, calcium, magnesium, iron, zinc, copper, manganese, and cadmium in root, stem, leaves and fruit. The results showed that cadmium (20 µM had significant effects on some measured growth indices. Growth parameters such as RGR, NAR, RLGR, Ls, RDW, RFW, SFW and SDW decreased 13.3, 19.1, 13.3, 18.8, 59.5, 58.7, 53.9 and 65.3%, respectively, and SLA increased 12.2%. But physiological parameters were not significantly affected by cadmium. The Cd concentration in all organs increased except fruit, compared with control which was Cd free. High cadmium concentration had significant effects on concentration of Mn and Cu in the root, Mn and P in the stem and Mn in the leaves. Concentration of Mn increased 5.3 and 87.6% in the leaves and stem, respectively, while it decreased 58.4% in the root. Concentration of P increased 33.3% and Cu increased 2times more in the stem. However, Ca concentration decreased 76.7% in the fruit, compared with the control. In conclusion, it is not recommended to grow tomato in the soils with high Cd concentration (polluted soil because of its negative effects on tomato growth.

  9. Effects of varying environmental parameters on trace contaminant concentrations in the NASA Space Station Reference Configuration

    Science.gov (United States)

    Brewer, Dana A.; Hall, John B., Jr.

    1986-01-01

    An evaluation is made of the NASA Space Station Reference Configuration trace contaminant production and depletion level effects of CO2, O2, humidity, temperature, and pressure variations, on the basis of a computer model of the Reference Configuration's chemical reactions and physical processes as functions of time. The effects of changes in the initial concentrations of such contaminants as nonmethane hydrocarbons and nitrogen oxides are also examined, and these are found to result in more significant changes in the concentration levels of trace contaminants than pressure and humidity variations. O2 and CO2 changes are found to have negligible effects on trace contaminant concentrations.

  10. The suitability of some blood gas and biochemical parameters as diagnostic tools or early indicators of ascites syndrome in broiler sire lines.

    Science.gov (United States)

    Hasanpur, K; Nassiri, M R; Hosseini Salekdeh, G; Vaez Torshizi, R; Pakdel, A; Kermanshahi, H; Naghous, M

    2016-06-01

    In recent few years, there have been some attempts to find a reliable indicator trait as a selection criterion against susceptibility to ascites syndrome (AS). Blood parameters were of great interest as they could be measured in live animals without implementing an ascites-inducing challenge (AIC). In this work, the suitability of some blood parameters was evaluated for diagnosing AS-susceptible chicks in later steps of the disease in trial 1 as well as their early predictive ability in trial 2. In the first trial, one hundred 1-day-old chicks from two pure broiler lines namely S1 and S2 and, in the second trial, 226 1-day-old chicks from line S2 were subjected to AIC. Saline drinking water (1200 mg/l) and lower-than-standard ambient temperatures were the implemented AICs in trials 1 and 2 respectively. The blood parameters including pH, partial pressure of O2 (pO2 ), partial pressure of CO2 (pCO2 ), bicarbonate ion concentration (BIC), percentage of haematocrit (HCT) and saturated haemoglobin (SaO2 ) were measured twice per each bird at days 28 and 35 in trial 1 and once in trial 2 at day 21. The results of the first trial revealed that in line S2 some of the blood parameters differed significantly between the ascitic and non-ascitic groups following exposure to AIC. In this line, the incidence of AS was accompanied by a lower pO2 , SaO2 and BIC, while with higher pCO2 and HCT values. In the second trial, however, although almost all of the parameters showed meaningful differences between the ascitic and non-ascitic broilers, only mean difference of BIC parameter was statistically significant. The general conclusion of this study is that the blood parameters can somewhat have diagnostic ability in the condition in which the AIC is already present, whereas the results did not approve their usefulness as early predictors of AS. PMID:26608233

  11. Predicting the activity coefficients of free-solvent for concentrated globular protein solutions using independently determined physical parameters.

    Directory of Open Access Journals (Sweden)

    Devin W McBride

    Full Text Available The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent model, which has previously been shown to provide excellent prediction of the osmotic pressure of concentrated and crowded globular proteins in aqueous solutions up to near-saturation concentrations. Thus, this model uses only the independently determined, physically realizable quantities: mole fraction, solvent accessible surface area, and ion binding, in its prediction. Predictions are presented for the activity coefficients of free-solvent for near-saturated protein solutions containing either bovine serum albumin or hemoglobin. As a verification step, the predictability of the model for the activity coefficient of sucrose solutions was evaluated. The predicted activity coefficients of free-solvent are compared to the calculated activity coefficients of free-solvent based on osmotic pressure data. It is observed that the predicted activity coefficients are increasingly dependent on the solute-solvent parameters as the protein concentration increases to near-saturation concentrations.

  12. Predicting the activity coefficients of free-solvent for concentrated globular protein solutions using independently determined physical parameters.

    Science.gov (United States)

    McBride, Devin W; Rodgers, Victor G J

    2013-01-01

    The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent model, which has previously been shown to provide excellent prediction of the osmotic pressure of concentrated and crowded globular proteins in aqueous solutions up to near-saturation concentrations. Thus, this model uses only the independently determined, physically realizable quantities: mole fraction, solvent accessible surface area, and ion binding, in its prediction. Predictions are presented for the activity coefficients of free-solvent for near-saturated protein solutions containing either bovine serum albumin or hemoglobin. As a verification step, the predictability of the model for the activity coefficient of sucrose solutions was evaluated. The predicted activity coefficients of free-solvent are compared to the calculated activity coefficients of free-solvent based on osmotic pressure data. It is observed that the predicted activity coefficients are increasingly dependent on the solute-solvent parameters as the protein concentration increases to near-saturation concentrations.

  13. Effects of temperature and discharge parameters on ozone concentration of negative corona discharge

    Institute of Scientific and Technical Information of China (English)

    WANG Hai-jun; SHENG Lian-xi; XU De-xuan

    2003-01-01

    The relationship of the ozone generation and the heating power, corona wire surface temperature, discharge electrode and netting electrode was studied during the negative corona discharge. The experimental results showed that the ozone concentration reduced with applied voltage decrease. With heating power increase, the ozone concentration of unit current decreased at exponential rate and it almost change no longer over 0.40 W. Under given temperature, the lower the applied voltage was, the smaller the ozone concentration was; while under given applied voltages, only over 11 kV could decrease with the surface temperature increase. The ozone concentration decreased with the lengthening of corona wire, and could reduced to 10 ppb under experimental condition of 14.2 kV; it also decreased with the shortening of wire diameter,and could decrease 67% at best in the given condition. Moreover, it decreased with the increasing size of netting electrode mesh. At the anion current of 1.65 μA, the ozone concentration of 5.0 × 5.0 cm2 is only 41% of that of 3.3 × 3.3 cm2 .

  14. Determination of effect factor for effective parameter on saccharification of lignocellulosic material by concentrated acid

    Science.gov (United States)

    Aghili, Sina; Nodeh, Ali Arasteh

    2015-12-01

    Tamarisk usage as a new group of lignocelluloses material to produce fermentable sugars in bio ethanol process was studied. The overall aim of this work was to establish the optimum condition for acid hydrolysis of this new material and a mathematical model predicting glucose release as a function of operation variable. Sulfuric acid concentration in the range of 20 to 60%(w/w), process temperature between 60 to 95oC, hydrolysis time from 120 to 240 min and solid content 5,10,15%(w/w) were used as hydrolysis conditions. HPLC was used to analysis of the product. This analysis indicated that glucose was the main fermentable sugar and was increase with time, temperature and solid content and acid concentration was a parabola influence in glucose production. The process was modeled by a quadratic equation. Curve study and model were found that 42% acid concentration, 15 % solid content and 90oC were optimum condition.

  15. Hematological and biochemical parameters of Dorper ewesParâmetros hematológicos e bioquímicos de ovinos da raça Dorper

    Directory of Open Access Journals (Sweden)

    Karina Medici Madureira

    2013-05-01

    Full Text Available In order to establish hematological and biochemical Dorper sheep values and evaluate the influence of sex and age in these determinations, 72 animals were assigned to three experimental groups according to age: Group 1 (G1 consisting of animals up to 12 months of age (n=28, Group 2 (G2 composed of animals from 13 to 36 months (n=37 and Group 3 (G3 for animals from 37 to 60 months (n=7. The animals were still divided in another two groups according to gender: Group 4 (G4 – females (n=57 and Group 5 (G5 – males (n=15. Evaluating the results obtained for hematological and biochemical examinations it was found that the mean values of RBC (13.4 ± 2.5x106/mL, hemoglobin (12.6 ± 1.3 g/dL, packed cell volume (37.5 ± 3.7%, total leukocytes (7116.0 ± 1967.0/mL and segmented neutrophils (4168.0 ± 1501.0/mL were higher in the animals of G1, as well as urea, GGT and ALP (52.0 ± 17.0 mg/dL, 93.0 ± 19.0 IU/L, 257.0 ± 125.0 IU/L, respectively. Comparing the gender, it was found that the values of total leukocytes (7576.0 ± 2082.0/mL, segmented neutrophils (4296.0 ± 1510.0/mL and lymphocytes (2428.0 ± 1084.0/mL were greater in males (G5. The values of creatinine (1.3 ± 0.4 mg/dL, serum total protein (6.3 ± 0.9 g/dL and globulins (3.7 ± 1.0 g/dL were higher in females (G4. Statistical analysis of the results allowed to determine reference values for hematological and biochemical constituents of Dorper sheep breed, and concluded that there were variations in these parameters influenced by gender and different age groups. A fim de estabelecer os valores hematológicos e bioquímicos de ovinos da raça Dorper e avaliar a influência do sexo e idade nestas determinações, 72 animais foram distribuídos em três grupos experimentais de acordo com a faixa etária, sendo o Grupo 1 (G1 composto por animais com até 12 meses de idade (n=28, o Grupo 2 (G2 composto por animais de 13 a 36 meses de idade (n=37 e o Grupo 3 (G3 por animais de 37 a 60 meses de

  16. concentration

    Directory of Open Access Journals (Sweden)

    Seth F. Oppenheimer

    1999-01-01

    Full Text Available We consider a model for biochemical oxygen demand (BOD in a semi-infinite river where the BOD is prescribed by a time varying function at the left endpoint. That is, we study the problem with a time varying boundary loading. We obtain the well-posedness for the model when the boundary loading is smooth in time. We also obtain various qualitative results such as ordering, positivity, and boundedness. Of greatest interest, we show that a periodic loading function admits a unique asymptotically attracting periodic solution. For non-smooth loading functions, we obtain weak solutions. Finally, for certain special cases, we show how to obtain explicit solutions in the form of infinite series.

  17. The physiological and biochemical responses of a medicinal plant (Salvia miltiorrhiza L. to stress caused by various concentrations of NaCl.

    Directory of Open Access Journals (Sweden)

    Zhao Gengmao

    Full Text Available Salvia miltiorrhiza, which is commonly known as Danshen, is a traditional Chinese herbal medicine. To illustrate its physiological and biochemical responses to salt stress and to evaluate the feasibility of cultivating this plant in saline coastal soils, a factorial experiment under hydroponic conditions was arranged on the basis of a completely randomised design with three replications. Five salinity treatments (0, 25, 50, 75 and 100 mM NaCl were employed in this experiment. The results showed that salinity treatments of <100 mM NaCl did not affect the growth of Salvia miltiorrhiza in a morphological sense, but significantly inhibit the accumulation of dry matter. Salinity treatments significantly decreased the Chl-b content but caused a negligible change in the Chl-a content, leading to a conspicuous overall decrease in the T-Chl content. The Na(+ content significantly increased with increasing hydroponic salinity but the K(+ and Ca(2+ contents were reversed, indicating that a high level of external Na(+ resulted in a decrease in both K(+ and Ca(2+ concentrations in the organs of Salvia miltiorrhiza. Salt stress significantly decreased the superoxide dismutase (SOD activity of Salvia miltiorrhiza leaves in comparison with that of the control. On the contrary, the catalase (CAT activity in the leaves markedly increased with the increasing salinity of the hydroponic solution. Moreover, the soluble sugar and protein contents in Salvia miltiorrhiza leaves dramatically increased with the increasing salinity of the hydroponic solution. These results suggested that antioxidant enzymes and osmolytes are partially involved in the adaptive response to salt stress in Salvia miltiorrhiza, thereby maintaining better plant growth under saline conditions.

  18. Effect of some Physiological Parameters in Female Local Sheep on some Blood Biochemical Components and Hormonal Levels During Pregnancy and Lactation

    International Nuclear Information System (INIS)

    The present study was carried out to determine effect of breed, days of estimation within breed, parity number of ewes, sex and type of embryo and regression on age and weight of ewes, gestation length and some blood biochemical and hormones levels during pregnancy or post - partum period. A total number of 60 ewes, 2 - 4 years of age with body weight ranged between 48 to 58 kg from breeding flock in Research Farm of Nuclear Research Centre, Atomic Energy Authority were used in this study. And they were divided into two equal groups according to their breed (Ossimi and Rahmani breed ewes). Blood samples of approximately 7 ml were collected during pregnancy at 10, 20, 30, 50, 100, 120, 140, and 145 days up to parturitions. After parturitions blood samples were taken at 30 and 60 days. Serum total protein, albumin and globulin were determined. In addition, plasma triiodothyronine (T3) and thyroxin (T4) were determined. The results showed that Ossimi ewes had higher pregnancy duration (150.94 days) than Rahmani ewes (149.49 days), the shorter length was observed in ewes of the first parity while the higher of gestation length was found in ewes of the second parity, ewes having female fetus had a longest gestation length while the shortest gestation length was found in ewes having both male and female twins. It was found that during pregnancy period Rahmani ewes had higher T3 and T4 means than Ossimi ones T3 and T4 plasma triiodothyronine (T3) level decreased up to 50 th day of the pregnancy period in Ossimi and Rahmani ewes then it sharply increased at the 145 th day of pregnancy period. In addition, plasma T3 level decreased significantly with increasing ewes parity. The highest level of plasma T3 level (73.50 ng/dl) was found in ewes having twins (male and female) fetus while those having female and having male were found to be 66.50 and 63.15 ng/dl, respectively. Ewes having single had higher values of T3 level compared with twins while the T4 levels concentration

  19. Variation in plasmonic (electronic) spectral parameters of Pr (III) and Nd (III) with varied concentration of moderators

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, Shubha, E-mail: shubhamishra03@gmail.com [School of Studies in Physics, Vikram University, Ujjain (M. P.) (India); Limaye, S. N., E-mail: snl222@yahoo.co.in [Department of Chemistry, Dr. H.S. Gour University, A Central University, Sagar (M.P.) (India)

    2015-07-31

    It is said that the -4f shells behave as core and are least perturbed by changes around metal ion surrounding. However, there are evidences that-4f shells partially involved in direct moderator interaction. A systematic investigation on the plasmonic (electronic) spectral studies of some Rare Earths[RE(III).Mod] where, RE(III) = Pr(III),Nd(III) and Mod(moderator) = Y(III),La(III),Gd(III) and Lu(III), increased moderator concentration from 0.01 mol dm{sup −3} to 0.025 mol dm{sup −3} keeping the metal ion concentration at 0.01mol dm{sup −3} have been carried out. Variations in oscillator strengths (f), Judd-Ofelt parameters (T{sub λ}),inter-electronic repulsion Racah parameters (δE{sup k}),nephelauxetic ratio (β), radiative parameters (S{sub ED},A{sub T},β{sub R},T{sub R}). The values of oscillator strengths and Judd-Ofelt parameters have been discussed in the light of coordination number of RE(III) metal ions, denticity and basicity of the moderators. The [RE(III).Mod] bonding pattern has been studies in the light of the change in Racah parameters and nephelauxetic ratio.

  20. POLYMORPHISM AT THE ADH AND ALPHA-GPDH LOCI IN DROSOPHILA-MELANOGASTER - EFFECTS OF REARING TEMPERATURE ON DEVELOPMENTAL RATE, BODY-WEIGHT, AND SOME BIOCHEMICAL PARAMETERS

    NARCIS (Netherlands)

    OUDMAN, L; VANDELDEN, W; KAMPING, A; BIJLSMA, R

    1991-01-01

    The role of developmental time in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster, and the relationship with weight and some biochemical characters, were investigated. Experimental strains were constructed with different combinations of Adh and alpha-Gpdh all

  1. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A new analytical method for the determination of urea-urease system based on biochemical reaction heat induced laser beam deflection is presented in this paper. With the method, the Michaelis constant (Km) of urease and apparent inhibition constant (Ki) of some metal ion inhibitors were measured respectively. This method was also used for the quantitative determination of metal ions with satisfactory result.

  2. Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice: Part II: Impact on specific chemical and biochemical quality parameters

    NARCIS (Netherlands)

    Vervoort, L.; Plancken, van der I.; Grauwet, T.; Timmermans, R.A.H.; Mastwijk, H.C.; Matser, A.M.; Hendrickx, M.E.; Loey, van A.

    2011-01-01

    The impact of thermal, high pressure (HP) and pulsed electric field (PEF) processing for mild pasteurization of orange juice was compared on a fair basis, using processing conditions leading to an equivalent degree of microbial inactivation. Examining the effect on specific chemical and biochemical

  3. Effect of Gonadoreline Administration on Blood Progesterone, Oestradiol 17b Concentration and Some Fertility Parameters in Angora Goats

    OpenAIRE

    ALAÇAM, Erol

    1999-01-01

    Various fertility parameters, plasma progesterone and oestradiol 17b ?concentrations were investigated in Angora Goats receiving gonadoreline immediately or on 12th day after breeding . A total 48 cyclic , pluriparous Angora Goats, 2-5 years of age were divided into 3 groups. The first group received 100 µg gonadorelin immediately after breeding. The second group received 100 µg gonadoreline on the 12th day after breeding and the third group receiving no injection served as the control. Ju...

  4. A new procedure for estimating the cell temperature of a high concentrator photovoltaic grid connected system based on atmospheric parameters

    International Nuclear Information System (INIS)

    Highlights: • Concentrating grid-connected systems are working at maximum power point. • The operating cell temperature is inherently lower than at open circuit. • Two novel methods for estimating the cell temperature are proposed. • Both predict the operating cell temperature from atmospheric parameters. • Experimental results show that both methods perform effectively. - Abstract: The working cell temperature of high concentrator photovoltaic systems is a crucial parameter when analysing their performance and reliability. At the same time, due to the special features of this technology, the direct measurement of the cell temperature is very complex and is usually obtained by using different indirect methods. High concentrator photovoltaic modules in a system are operating at maximum power since they are connected to an inverter. So that, their cell temperature is lower than the cell temperature of a module at open-circuit voltage since an important part of the light power density is converted into electricity. In this paper, a procedure for indirectly estimating the cell temperature of a high concentrator photovoltaic system from atmospheric parameters is addressed. Therefore, this new procedure has the advantage that is valid for estimating the cell temperature of a system at any location of interest if the atmospheric parameters are available. To achieve this goal, two different methods are proposed: one based on simple mathematical relationships and another based on artificial intelligent techniques. Results show that both methods predicts the cell temperature of a module connected to an inverter with a low margin of error with a normalised root mean square error lower or equal than 3.3%, an absolute root mean square error lower or equal than 2 °C, a mean absolute error lower or equal then 1.5 °C, and a mean bias error and a mean relative error almost equal to 0%

  5. A hepcidina como parâmetro bioquímico na avaliação da anemia por deficiência de ferro Hepcidin as a biochemical parameter for the assessment of iron deficiency anemia

    Directory of Open Access Journals (Sweden)

    Andrea dos Reis Lemos

    2010-01-01

    parameter for assessment of iron deficiency anemia is reported in this review. A literature review was carried out in the databases PubMed and LILACS, between 2006-2010, assessing hepcidin as a parameter for the regulation of iron metabolism. During this period 35 studies were published in international journals and one study in a Brazilian journal. The production of hepcidin is homeostatically regulated by anemia and hypoxia. When oxygen supply is inadequate the level of hepcidin decreases. Therefore, more iron from the diet and from the stock of macrophages and hepatocytes becomes available. Hepcidin links to ferroportin, regulating iron release to plasma. When the hepcidin concentrations are low, the molecules of ferroportin are exposed on the plasmatic membrane and release iron. When hepcidin levels increase, hepcidin binds to molecules of ferroportin inducing its internalization and degradation and iron release gradually decreases. Apparently, development of diagnosis and therapy for anemia based on the parameter hepcidin may provide a more effective approach. Large-studies are needed to demonstrate the importance of hepcidin for the differential diagnosis of anemia, including sample protocols for analysis, with standards similar to those used in other biochemical evaluations, as well as the definition of cut-off points for the plasma and urinary expression of this peptide.

  6. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    Science.gov (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver.

  7. Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples.

    Science.gov (United States)

    Levitas, E; Lunenfeld, E; Weisz, N; Friger, M; Potashnik, G

    2007-04-01

    This study evaluates retrospectively the relationship between age and semen parameters among men with normal sperm concentration. It was based on computerized data and performed in an Academic Fertility and IVF Unit. Six thousand and twenty-two semen samples with sperm concentrations of >or=20 x 10(6) ml(-1) were examined according to WHO criteria and analysed in relation to patients' age. For each age group, mean values +/- SD of semen volume, sperm concentration, percentage of motile spermatozoa, normal morphology, acrosome index, total sperm count/ejaculate, total motile sperm count/ejaculate and sexual abstinence duration were examined. A peak semen volume of 3.51 +/- 1.76 ml(-1) was observed at age >or=30 to age >or=55 years (PSperm motility was found to be inversely related to age with peak motility of 44.39 +/- 20.69% at age age >or=55 years (Psperm, between values of 103.34 +/- 107 x 10(6) at age >or=30 to age >55 years. A statistically significant and inverse relationship was observed between semen volume, sperm quality and patient age, in spite of prolonged sexual abstinence duration. Top sperm parameters were observed at age >or=30 to sperm parameters occurred after the age of 55 years.

  8. Effect of concentration of dispersed organic matter on optical maturity parameters: Interlaboratory results of the organic matter concentration working group of the ICCP.

    Science.gov (United States)

    Mendonca, Filho J.G.; Araujo, C.V.; Borrego, A.G.; Cook, A.; Flores, D.; Hackley, P.; Hower, J.C.; Kern, M.L.; Kommeren, K.; Kus, J.; Mastalerz, Maria; Mendonca, J.O.; Menezes, T.R.; Newman, J.; Ranasinghe, P.; Souza, I.V.A.F.; Suarez-Ruiz, I.; Ujiie, Y.

    2010-01-01

    The main objective of this work was to study the effect of the kerogen isolation procedures on maturity parameters of organic matter using optical microscopes. This work represents the results of the Organic Matter Concentration Working Group (OMCWG) of the International Committee for Coal and Organic Petrology (ICCP) during the years 2008 and 2009. Four samples have been analysed covering a range of maturity (low and moderate) and terrestrial and marine geological settings. The analyses comprise random vitrinite reflectance measured on both kerogen concentrate and whole rock mounts and fluorescence spectra taken on alginite. Eighteen participants from twelve laboratories from all over the world performed the analyses. Samples of continental settings contained enough vitrinite for participants to record around 50 measurements whereas fewer readings were taken on samples from marine setting. The scatter of results was also larger in the samples of marine origin. Similar vitrinite reflectance values were in general recorded in the whole rock and in the kerogen concentrate. The small deviations of the trend cannot be attributed to the acid treatment involved in kerogen isolation but to reasons related to components identification or to the difficulty to achieve a good polish of samples with high mineral matter content. In samples difficult to polish, vitrinite reflectance was measured on whole rock tended to be lower. The presence or absence of rock fabric affected the selection of the vitrinite population for measurement and this also had an influence in the average value reported and in the scatter of the results. Slightly lower standard deviations were reported for the analyses run on kerogen concentrates. Considering the spectral fluorescence results, it was observed that the ??max presents a shift to higher wavelengths in the kerogen concentrate sample in comparison to the whole-rock sample, thus revealing an influence of preparation methods (acid treatment) on

  9. In vitro bioassays for anticancer drug screening: effects of cell concentration and other assay parameters on growth inhibitory activity.

    Science.gov (United States)

    Lieberman, M M; Patterson, G M; Moore, R E

    2001-11-01

    In vitro growth inhibition assays were performed using human cancer cell lines at various concentrations with experimental anticancer drugs such as the cryptophycins and other cytotoxins. The effect of variations in assay parameters on the observed growth inhibition of these anticancer therapeutic agents was determined. The results demonstrated that the observed inhibitory activity of these compounds varied inversely with the cell concentrations used. The observed differences in activity between different cytotoxins were not necessarily proportionate. Thus, the relative activities of two toxins also varied with cell concentration. Furthermore, the sensitivity of these cell lines to the cytostatic purine analog, 6-mercaptopurine (used as a control), varied with cell concentration as well. The activity of this compound was dependent on the medium used for cell growth, yielding good activity in Eagle's minimum essential medium, but not in Ham's F-12 (Kaigin) medium. Moreover, growth inhibition by cryptophycin as well as 6-mercaptopurine was also dependent on the serum concentration in the medium. Finally, the sensitivity of the cancer cell lines to various organic solvents commonly used as drug vehicles for in vitro testing, such as ethanol, dimethylformamide, and dimethylsulfoxide, was likewise found to vary inversely with cell concentration. PMID:11578805

  10. Relationship between Serum Umbilical Cord and Maternal Leptin and Adiponectin Concentrations with Fetal Growth Parameters

    Directory of Open Access Journals (Sweden)

    F Zare

    2007-08-01

    Full Text Available Background: Pregnancy and accelerated fetal growth always are related with major metabolic changes and body fat redistribution and adiponectin is one of principle adipocyte hormones, so studying adiponectin changes during pregnancy may reveal some hidden parts of fetal metabolism. The aim of this study was to assess adiponectin and leptin levels in umbili¬cal cord and maternal serum, their relation with each other and with neonatal weight, birth length and other fetal growth markers. Methods: The study was carried out with 72 appropriate for age newborns (36 female, 36 male and their mothers. The anthropometric variables of the newborns studied were birth weight, birth length, and birth weight/birth length and ponderal in¬dex. Maternal and umbilical cord adiponectin and leptin levels were measured by ELISA and compared. Results: The median of cord blood adiponectin concentration were 3 fold higher than those of maternal group. Umbilical cord blood leptin levels were significantly correlated with neonatal birth weight and birth weight/ birth length (r= 0.29, P= 0.01 and r= 0.24, P= 0.04, respectively. No statistical difference has been demonstrated between both groups of male and fe¬male neonates regarding birth weight, birth length, maternal and neonatal leptin levels, ponderal index and maternal and neona¬tal adiponectin levels. Conclusion: Neonatal leptin is related to birth weight. Adiponectin has no relation with birth weight. Neither leptin nor adi¬ponectin correlated with gender difference.

  11. Effect of environmental parameters on pathogen and faecal indicator organism concentrations within an urban estuary

    Science.gov (United States)

    Henry, Rebekah; Schang, Christelle; Kolotelo, Peter; Coleman, Rhys; Rooney, Graham; Schmidt, Jonathan; Deletic, Ana; McCarthy, David T.

    2016-06-01

    Current World Health Organisation figures estimate that ∼2.5 million deaths per year result from recreational contact with contaminated water sources. Concerns about quantitative risk assessments of waterways using faecal indicator organisms (FIOs) as surrogates to infer pathogenic risk currently exist. In Melbourne, Australia, the Yarra River has come under public scrutiny due to perceived public health risks associated with aquatic recreation; a characteristic shared with urban estuaries worldwide. A 10-month study of the Yarra estuary investigated the processes that affect FIOs and pathogens within this system. A total of 74 samples were collected from three estuarine and two upstream, freshwater, locations under different climatic and hydrological conditions, and the levels of Escherichia coli, enterococci, Clostridium perfringens, fRNA coliphages, Campylobacter spp. Cryptosporidium oocysts, Giardia cysts, adenoviruses, and enteroviruses were monitored. Reference pathogenic bacteria, protozoa, and viruses were detected in 81%, 19%, and 8% of samples, respectively. Variations in FIO concentrations were found to be associated with changes in specific climatic and hydrological variables including: temperature, flow, humidity and rainfall. In contrast, pathogen levels remained unaffected by all variables investigated. Limitations of current national and international culture-based standard methods may have played a significant role in limiting the identification of correlative relationships The data demonstrate the differences between FIOs and microbial pathogens in terms of sources, sinks, and survival processes within an urban estuary and provide further evidence of the inadequacy of FIO inclusion in the development of worldwide regulatory water quality criteria and risk assessment models.

  12. Effect of Porosity and Concentration Polarization on Electrolyte Diffusive Transport Parameters through Ceramic Membranes with Similar Nanopore Size

    Directory of Open Access Journals (Sweden)

    Virginia Romero

    2014-08-01

    Full Text Available Diffusive transport through nanoporous alumina membranes (NPAMs produced by the two-step anodization method, with similar pore size but different porosity, is studied by analyzing membrane potential measured with NaCl solutions at different concentrations. Donnan exclusion of co-ions at the solution/membrane interface seem to exert a certain control on the diffusive transport of ions through NPAMs with low porosity, which might be reduced by coating the membrane surface with appropriated materials, as it is the case of SiO2. Our results also show the effect of concentration polarization at the membrane surface on ionic transport numbers (or diffusion coefficients for low-porosity and high electrolyte affinity membranes, which could mask values of those characteristic electrochemical parameters.

  13. Changes in the concentrations of biochemical indicators of diet and nutritional status of pregnant women across pregnancy trimesters in Trujillo, Peru, 2004–2005

    OpenAIRE

    Horton, D. Kevin; Adetona, Olorunfemi; Aguilar-Villalobos, Manuel; Cassidy, Brandon E; Pfeiffer, Christine M; Schleicher, Rosemary L.; Caldwell, Kathleen L; Needham, Larry L.; Rathbun, Stephen L.; Vena, John E.; Naeher, Luke P.

    2013-01-01

    Background In developing countries, deficiencies in essential micronutrients are common, particularly in pregnant women. Although, biochemical indicators of diet and nutrition are useful to assess nutritional status, few studies have examined such indicators throughout pregnancy in women in developing countries. Methods The primary objective of this study was to assess the nutritional status of 78 Peruvian women throughout pregnancy for 16 different nutritional indicators including fat-solubl...

  14. Phase separation of polymer solutions. The calculation of the cloudpoint curve with a concentration and temperature-dependent free energy correction parameter

    NARCIS (Netherlands)

    Emmerik, van P.T.; Smolders, C.A.

    1973-01-01

    The free enthalpy correction parameter g in the Flory-Huggins equation for the Gibbs free enthalpy of mixing in polymer solutions is considered generally as a concentration- and temperature-independent parameter. It has been extended here with linear concentration- and temperature-dependent terms. W

  15. Blind Deconvolution for Distributed Parameter Systems with Unbounded Input and Output and Determining Blood Alcohol Concentration from Transdermal Biosensor Data.

    Science.gov (United States)

    Rosen, I G; Luczak, Susan E; Weiss, Jordan

    2014-03-15

    We develop a blind deconvolution scheme for input-output systems described by distributed parameter systems with boundary input and output. An abstract functional analytic theory based on results for the linear quadratic control of infinite dimensional systems with unbounded input and output operators is presented. The blind deconvolution problem is then reformulated as a series of constrained linear and nonlinear optimization problems involving infinite dimensional dynamical systems. A finite dimensional approximation and convergence theory is developed. The theory is applied to the problem of estimating blood or breath alcohol concentration (respectively, BAC or BrAC) from biosensor-measured transdermal alcohol concentration (TAC) in the field. A distributed parameter model with boundary input and output is proposed for the transdermal transport of ethanol from the blood through the skin to the sensor. The problem of estimating BAC or BrAC from the TAC data is formulated as a blind deconvolution problem. A scheme to identify distinct drinking episodes in TAC data based on a Hodrick Prescott filter is discussed. Numerical results involving actual patient data are presented.

  16. Uncertainty of Blood Alcohol Concentration (BAC Results as Related to Instrumental Conditions: Optimization and Robustness of BAC Analysis Headspace Parameters

    Directory of Open Access Journals (Sweden)

    Haleigh A. Boswell

    2015-12-01

    Full Text Available Analysis of blood alcohol concentration is a routine analysis performed in many forensic laboratories. This analysis commonly utilizes static headspace sampling, followed by gas chromatography combined with flame ionization detection (GC-FID. Studies have shown several “optimal” methods for instrumental operating conditions, which are intended to yield accurate and precise data. Given that different instruments, sampling methods, application specific columns and parameters are often utilized, it is much less common to find information on the robustness of these reported conditions. A major problem can arise when these “optimal” conditions may not also be robust, thus producing data with higher than desired uncertainty or potentially inaccurate results. The goal of this research was to incorporate the principles of quality by design (QBD in the adjustment and determination of BAC (blood alcohol concentration instrumental headspace parameters, thereby ensuring that minor instrumental variations, which occur as a matter of normal work, do not appreciably affect the final results of this analysis. This study discusses both the QBD principles as well as the results of the experiments, which allow for determination of more favorable instrumental headspace conditions. Additionally, method detection limits will also be reported in order to determine a reporting threshold and the degree of uncertainty at the common threshold value of 0.08 g/dL. Furthermore, the comparison of two internal standards, n-propanol and t-butanol, will be investigated. The study showed that an altered parameter of 85 °C headspace oven temperature and 15 psi headspace vial pressurization produces the lowest percent relative standard deviation of 1.3% when t-butanol is implemented as an internal standard, at least for one very common platform. The study also showed that an altered parameter of 100 °C headspace oven temperature and 15-psi headspace vial pressurization

  17. Effect of calcium soap of fatty acids supplementation on serum biochemical parameters and ovarian activity during out-of-the-breeding season in crossbred ewes.

    Science.gov (United States)

    El-Nour, Hayat H M; Nasr, Soad M; Hassan, Walid R

    2012-01-01

    This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA) supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45-55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (P breeding season.

  18. EVALUATION OF FROZEN SEMEN BY A CROSOMAL INTEGRITY AND SPERM CONCENTRATION - TWO VITAL QUALITY PARAMETERS OF MALE FERTILITY IN BOVINES

    Directory of Open Access Journals (Sweden)

    Sumit Chowdhury

    2014-06-01

    Full Text Available Acrosomal integrity and sperm concentration are two important parameters to assess the quality of frozen semen doses which in terms validates the fertilizing capacity and conception rate. The present study was undertaken to evaluate acrosomal integrity by Giemsa’s stain and sperm concentration of FSS using improved neubauer chamber in Exotic pure Jersey, Crossbred Jersey, Indigenous Gir cattle and Indigenous Murrah buffalo prior to the field use. The overall values of Giemsa’s stain were observed as 73.74±0.31, 18.65±0.33 and 7.79±0.25 percent for Intact Acrosome, Partially Damaged Acrosome and Fully Damaged Acrosome, respectively. Overall values of sperm concentration were 21.98±0.28 million per straw. The study indicated that there was no significant difference (P<0.05 among the breeds and the values mostly correlates with the guideline of Minimum Standard Protocol for Production of bovine semen, 2012 of Govt. of India.

  19. Parameters of fermentation and rumen microbiota of Nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets.

    Science.gov (United States)

    Ribeiro Júnior, Carlos S; Messana, Juliana D; Granja-Salcedo, Yury T; Canesin, Roberta C; Fiorentini, Giovani; San Vito, Elias; Furlan, Luiz R; Reis, Ricardo A; Berchielli, Telma T

    2016-10-01

    The objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in Nellore steers. Eight rumen-cannulated Nellore steers (331 ± 8 kg BW) were distributed in a double 4 × 4 Latin square design balanced for the control of the residual effect. The ruminal pH decreased (p protozoa count revealed a predominance of the genus Entodinium. The synthesis of microbial N [g/d] and the efficiency of microbial synthesis [g of microbial N/kg of organic matter apparently digested in the rumen] increased as the proportion of concentrate was increased (p < 0.05). Therefore, it can be concluded that an increasing proportion of concentrate in sugarcane-containing diets enhances the synthesis of microbial protein and does not alter the fibre digestibility, although the population of fibre fermenting bacteria was reduced. PMID:27415825

  20. THE IN-VIVO EFFECTS OF PARTIALLY PURIFIED SUB-FRACTION (TAF2 OF THE CRUDE METHANOLIC EXTRACT OF EURYCOMA LONGIFOLIA ROOTS ON THE HAEMATOLOGICAL, BIOCHEMICAL AND HISTOLOGY PARAMETERS

    Directory of Open Access Journals (Sweden)

    Narazah Mohd Yusoff et al

    2012-09-01

    Full Text Available Eurycoma longifolia (E. longifolia Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial, anti-pyretic and anticancer activities. As yet, no study has been done on biosafety of partially purified sub-fraction (TAF2 of the crude methanolic extract of E. longifolia roots. This study aims to evaluate, in vivo, the effects of TAF2 on the haematological, biochemical and histology parameters in nude mice. Male nude mice were treated orally with TAF2 (200 mg/Kg/mouse every other day for 16 days. Untreated mice were used as controls. Blood samples were collected by intra-cardiac puncture and haematological and biochemical parameters were analyzed by using animal blood counter and automated chemistry analyzer, respectively. Hematoxylin-eosin stain (H&E was used to evaluate the histopathology changes. Blood cells morphology was assessed by Wright stain of the thin blood films. The results showed normal blood haematology and clinical chemistry. Histological examination of TAF2 treated vital organs (kidney, liver and spleen of mice showed no apparent change with the dose used. In conclusion, E. longifolia root methanolic extract is safe on short-term exposur.

  1. 晚期血吸虫病肝纤维化程度与生化指标的相关性%Correlation between levels of liver fibrosis and liver fibrosis biochemical pa-rameters of advanced schistosomiasis patients

    Institute of Scientific and Technical Information of China (English)

    吴一鸣; 徐晓菲; 乌文琳; 邬万新; 高树兴; 朱文军

    2014-01-01

    目的:探讨晚期血吸虫病肝纤维化程度与肝纤维化生化指标之间的相关性。方法以48例晚期血吸虫病肝病患者为研究对象,行肝活检及B超影像学检查,同时检测谷氨酰胺转肽酶(GGT)、碱性磷酸酶(AKP)、Ⅲ型前胶原(PC-Ⅲ)、Ⅳ型胶原(Ⅳ-C)、透明质酸(HA)及层粘连蛋白(LN)等肝纤维化生化指标。分别以肝活检病理结果和B超影像学检查确定肝纤维化程度,分析肝纤维化程度与上述肝纤维化生化指标间的相关性。结果肝活检病理检查确定的肝纤维化程度与各生化指标之间无相关性;B超影像学检查确定的肝纤维化程度与GGT、AKP、LN和PC-Ⅲ之间有一定相关性,与HA和Ⅳ-C呈显著相关。结论 B超检查是一种较好的无创性血吸虫病肝纤维化检查方法,结合肝纤维化生化指标能更好地反映肝脏整体状况。%Objective To explore the correlation between the levels of liver fibrosis and liver fibrosis biochemical parameters of advanced schistosomiasis patients. Methods A total of 48 advanced schistosomiasis patients were investigated and they were examined by the liver biopsy and B ultrasound imaging. At the same time,the liver fibrosis biochemical parameters,including glu-tamine transpeptidase(GGT),alkaline phosphatase(AKP),procollagenⅢ(PC-Ⅲ),collagen typeⅣ(Ⅳ-C),hyaluronic acid (HA)and laminin(LN),were detected. The liver fibrosis levels were classified by the liver biopsy and B ultrasound imaging,re-spectively,and the correlation between the levels of liver fibrosis and liver fibrosis biochemical parameters were analyzed statisti-cally. Results There was no correlation between the liver fibrosis levels classified by the liver biopsy and all the liver fibrosis bio-chemical parameters;there was a weak correlation between the liver fibrosis levels classified by the B ultrasound imaging and GGT,AKP,LN and PC-Ⅲ,respectively;there was a significant

  2. The concentrations of methaemoglogin, carboxyhaemoglobin and some haematological parameters in tobacco snuff addicts in Igbo of Nigeria.

    Science.gov (United States)

    Ureme, S O; Ibeagha, I D; Maduka, I G; Ibeagbulam, O G

    2007-01-01

    Methaemoglobin, carboxyhaemoglobin concentrations and some haematological parameters were studied in fifty tobacco snuff addicts (40 males and 10 females) in some villages of Anambra State, Nigeria. The aim was to investigate possible adverse effects of tobacco snuff in addicts in Igbos of Anambra State. Fifty apparently healthy persons (25 males and 25 females) who do not inhale snuff were used as controls. The age range of tests and control subjects was 25-65 years. The results showed no statistically significant difference when the tests group was compared with the control group. A comparison of the results on the basis of sex, age and period of exposure, showed no significant differences. Blood picture in test and control groups was normocytic and normochromic. The results suggest that tobacco snuff may not have any adverse effect on haemoglobin metabolism and erythropoiesis. PMID:18379614

  3. Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

    Directory of Open Access Journals (Sweden)

    Nilton Massuo Ishikawa

    2007-07-01

    Full Text Available Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758 was investigated by the hematological parameters after long-term (14 days exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg. Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC, hemoglobin concentration (Hb, hematocrit (Ht, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, mean corpuscular hemoglobin concentration (MCHC, total white blood cell count (WBC and differential leukocyte counts and total thrombocyte count (Tr. There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.A toxicidade do mercúrio foi avaliada em tilápia, Oreochromis niloticus (Linnaues, 1758 através da análise dos parâmetros hematológicos após exposição a diferentes concentrações sub-letais, durante um período de 14 dias. O bioensaio foi conduzido no laboratório de toxicologia do Instituto de Pesca, SP. Foram utilizados alevinos (12.44 ± 0.84 cm, e 27.13 ± 4.67 g e aquários com capacidade para 50 litros e preenchidos com água declorada e mais a quantidade de solução de mercúrio (HgCl2 correspondendo as seguintes concentrações: 0,02; 0,002; 0.0002 mg.L-1 Hg. Foram utilizadas 3 repetições de cada concentração e grupo controle. Amostras de sangue foram coletadas de seis animais de cada concentração nos tempos 0, 3, 7, 10 e 14 dias de exposição. Foram avaliados: a contagem de eritrócitos (RBC, concentração de hemoglobina (Hb, hematócrito (Ht, volume corpuscular médio (VCM, hemoglobina corpuscular média (HCM e concentração de hemoglobina corpuscular média (CHCM, trombócitos totais (Tr, contagem

  4. Calorie restriction in biosphere 2: alterations in physiologic, hematologic, hormonal, and biochemical parameters in humans restricted for a 2-year period.

    Science.gov (United States)

    Walford, Roy L; Mock, Dennis; Verdery, Roy; MacCallum, Taber

    2002-06-01

    Four female and four male crew members, including two of the present authors (R. Walford and T. MacCallum)--seven of the crew being ages 27 to 42 years, and one aged 67 years--were sealed inside Biosphere 2 for two years. During seven eighths of that period they consumed a low-calorie (1750-2100 kcal/d) nutrient-dense diet of vegetables, fruits, nuts, grains, and legumes, with small amounts of dairy, eggs, and meat (approximately 12% calories from protein, approximately 11% from fat, and approximately 77% from complex carbohydrates). They experienced a marked and sustained weight loss of 17 +/- 5%, mostly in the first 8 months. Blood was drawn before entry into Biosphere 2, at many time-points inside it, and four times during the 30 months following exit from it and return to an ad libitum diet. Longitudinal studies of 50 variables on each crew member compared outside and inside values by means of a Bayesian statistical analysis. The data show that physiologic (e.g., body mass index, with a decrease of 19% for men and 13% for women; blood pressure, with a systolic decrease of 25% and a diastolic decrease of 22%), hematologic (e.g., white blood cell count, decreased 31%), hormonal (e.g., insulin, decreased 42%; T3, decreased 19%), biochemical (e.g., blood sugar, decreased 21%; cholesterol, decreased 30%), and a number of additional changes, including values for rT3, cortisol, glycated hemoglobin, plus others, resembled those of rodents or monkeys maintained on a calorie-restricted regime. Significant variations in several substances not hitherto studied in calorie-restricted animals are also reported (e.g., androstenedione, thyroid binding globulin, renin, and transferrin). We conclude that healthy nonobese humans on a low-calorie, nutrient-dense diet show physiologic, hematologic, hormonal, and biochemical changes resembling those of rodents and monkeys on such diets. With regard to the health of humans on such a diet, we observed that despite the selective

  5. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose pdiabetes mellitus prevalence were seen too much.

  6. Effect of treating lactating rats with lead acetate and its interaction with vitamin E or C on neurobehavior, development and some biochemical parameters in their pups

    Directory of Open Access Journals (Sweden)

    A. A. Hassan

    2010-01-01

    Full Text Available The current study investigated the effect of administration of vitamin E (600mg/ kg diet concomitantly with lead acetate (10mg/kg, orally and vitamin C (100mg/kg, orally concomitantly with lead acetate (10mg/kg, orally to the female lactating rats on the neurobehavioral, landmarks development and some biochemical tests in their pups. Administration of lead acetate to the female lactating rats caused a significant increase in open field activity test including (the number of squares crossed and rearing test within 3 minutes, olfactory discrimination test, triglycerides and malondialdehyde brain tissue, with a significant decrease in glutathione brain tissue and high density lipoproteins in their pups. The present study demonstrated that treatment of female lactating rats with vitamin C and lead acetate produced a significant decrease in righting reflex test in their pups. Administration of vitamin E concomitantly with lead acetate to the female lactating rats caused a significant increase in glutathione level accompanied with a significant decrease in malondialdehyde and triglycerides levels in their pups. The present study showed that treatment of female lactating rats with vitamin E or C with lead acetate produced a significant decrease in rearing test, whereas a significant increase in high density lipoproteins in their pups. It is concluded that administration vitamin E or C to the female lactating rats reverse the adverse effects produced by lead acetate on neurobehavioral. Vitamin E had positive effect on the levels of glutathione, malondialdehyde brain tissue, triglyceride and high density lipoproteins in their lactating pups.

  7. Hepato Protective Efficacy of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and Their Formulation on Imidacloprid Induced Liver Toxicity by Histopathological and Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    V. Eugin Amala

    2015-09-01

    Full Text Available Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations are used as a phytotherapeutic medicine for hepatic diseases. In the present study the effect of methanolic extract of herbal powder was administered against Imidacloprid in Albino Wistar Rats. Single dose of herbal (500 mg/kg/b.w was given orally for 28 days to Imidacloprid toxicity exposed to rats. They were observed each hour and each day for 28 days for any changes in behavioural activity loss of ability to move, cramping, lethargy, muscle weakness, convulsion, irritation of the eye, excess salivation, change in gait. Clinical observations body weight, organ weight, biochemical analysis and histopathological examination were carried out. Administration of Imidacloprid, produced liver degeneration such as cytoplasmic vacuolation, mild vacuolar degeneration, mild hepatic damage in low dose (40 mg/kg b.w and appearance of blood streaks, hepatic damage and severe vacuolar degenerations were observed in high dose of induction (80 mg/kg b.w. Animals were treated with the phytotherapeutic products of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations. Triphala extract was possessed more hepatoprotective activity, while comparing the four samples which was proved experimentally.

  8. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose pblood biochemistry levels were observed statisticaly different pgroup. In conclusion, obese and obese+diabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.

  9. Effect of Milk Thistle (Silybum marianum L. on Biochemical Parameters and Immunity of Broiler Chicks Fed Aflatoxin B1 after Three Weeks

    Directory of Open Access Journals (Sweden)

    Maliheh Amiri Dumari

    2014-09-01

    Full Text Available Background: This study was conducted to determine the efficacy of milk thistle seeds (MTSs in counteracting the toxic effects of aflatoxin B1 (AFB1 in a contaminated diet fed to broilers. Methods: Two dietary inclusion rates of AFB1 (0, 0.250 and 500 ppb and MTS (0, 0.5 and 1% were tested in a 3×3 factorial manner. The effect of nine experimental treatments was assessed using 216 one-d-old Ross 308 male broiler chicks in a randomized complete design with 4 replicates of 6 birds each from one to 21 days of age. The effects of dietary AFB1 and MTS on serum biochemistry factors, antibody titer against Newcastle disease (ND and influenza disease (ID in broilers were evaluated at the end of this period. Results: Statistical analysis of the main effects of diets indicated no significant changes in uric acid, cholesterol, triglycerides, low density lipoprotein (LDL, ID, and phosphorus compared to the control (P>0.01. Also, addition of 500 ppb of dietary AFB1 into the diet was associated with significant decreases in serum glucose, calcium, high density lipoprotein (HDL, and ND compared to the control group (P<0.01. The contaminated diet significantly increased the activities of aspartate aminotransferase (AST and alanine aminotransferase (ALT (P<0.05. Conclusion: Milk thistle showed protective effects and resulted in some serum enzyme activities and serum biochemical changes associated with aflatoxin toxicity.

  10. Effect of Calcium Soap of Fatty Acids Supplementation on Serum Biochemical Parameters and Ovarian Activity during Out-of-the-Breeding Season in Crossbred Ewes

    Directory of Open Access Journals (Sweden)

    Hayat H. M. El-Nour

    2012-01-01

    Full Text Available This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45–55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (<0.05 increases in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and progesterone levels with decrease in calcium and phosphorous levels in treated group. In treated group, normal-size ovaries and more than one follicle on the ovaries were detected and pregnancy rate increased. In conclusion, CSFA supplementation was effective to maintain the reproductive performance when ewes were out of the breeding season.

  11. Leaf photosynthesis and respiration of three bioenergy crops in relation to temperature and leaf nitrogen: how conserved are biochemical model parameters among crop species?

    NARCIS (Netherlands)

    Archontoulis, S.V.; Yin, X.; Vos, J.; Danalatos, N.G.; Struik, P.C.

    2012-01-01

    Given the need for parallel increases in food and energy production from crops in the context of global change, crop simulation models and data sets to feed these models with photosynthesis and respiration parameters are increasingly important. This study provides information on photosynthesis and r

  12. [b][/b]Impact of copper (Cu at the dose of 50 mg on haematological and biochemical blood parameters in turkeys, and level of Cu accumulation in the selected tissues as a source of information on product safety for consumers

    Directory of Open Access Journals (Sweden)

    Bogusław Makarski

    2014-09-01

    Full Text Available Introduction. The current state-of the art points to a positive impact of copper (Cu supplements on the general health status in poultry. Copper induces beneficial changes in the haematological and biochemical blood parameters. It also displays immunostimulating properties and helps maintain a proper microbiological balance in the digestive tract. [b]Objective[/b]. The objective of this study was to investigate the impact of Cu at the dose of 50 mg/kg BW, administered in organic and inorganic form, on the haematological and biochemical blood parameters and level of Cu bioaccumulation in the liver and pectoral muscle. [b]Materials and method[/b]. The study was carried out on 45 BUT-9 turkeys which had been were reared for 16 weeks. They were divided into 3 experimental groups: I – the control group; II – fed with CuSO[sub]4[/sub] at the dose of 50 mg Cu•dm [sup]-3 [/sup]H[sub]2[/sub]O; III – received a Cu chelate with lysine at the same dose. [b]Results[/b]. The administration of Cu at the dose exceeding the nutritional recommendations did not induce beneficial changes in the examined birds. This indicates that it is not necessary to administer Cu doses higher than the recommended levels. The extent of Cu accumulation in the pectoral muscle increased by 40% compared to the control group, whereas in the liver it was higher by 30–35% than in the birds without Cu administration. The level of Cu in tissues does not pose a risk to consumers. [b]Conclusions[/b]. The supplementation of Cu at the dose of 50 mg has a negative impact on the level of the analyzed parameters. The results of the presented study indicate that the administered Cu dose exceeds birds’ demand for this element.

  13. Effects of exposure to three environmental chemicals on the selected biochemical parameters of the blood plasma of rainbow trout, Oncorhynchus mykiss (Walbaum

    Directory of Open Access Journals (Sweden)

    Řehulka Jiří

    2016-03-01

    Full Text Available Rainbow trout Oncorhynchus mykiss at a weight of 115 ± 24 g (mean ± SD were experimentally injected with di(2-ethylhexylphthalate (DEHP, 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD and gamma isomer of hexachlorocyclohexane (γ-HCH to evaluate nitrogen metabolism (total protein [TP], blood urea nitrogen, uric acid [UA], creatinine, carbohydrate metabolism (glucose, mineral metabolism (inorganic phosphate[P], total calcium [Cat], and catalytic activity of enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], lactate dehydrogenase [LD]. After 21 days, the tested xenobiotics, administered by the intraperitoneally route, caused the following effect: in comparison with negative controls, fish injected with DEHP at concentrations of 200 or 50 mg kg-1 of body weight were found to have a higher level of P and lower level of Cat and decreased catalytic concentrations of ALT, AST, ALP and LD in the plasma of the peripheral blood. A higher level of TP and P and a decreased catalytic concentration of ALT were found when TCDD was administered at a dose of 2 μg kg-1. A decrease in the catalytic concentrations of ALT and ALP occurred in fish injected with γ-HCH in a dose of 50 mg kg-1. UA and P levels were decreased in fish injected with γ-HCH in a dose of 5 mg kg-1. DEHP and TCDD caused neutrophilic leucocytosis with a marked left shift. Both concentrations of γ-HCH led to an increase in polychromatophilic erythrocytes. The frequency of micronuclei varied in all experimental groups, including the controls, ranging between 0.8 and 2.1 ‰. Histological examination in fish injected with DEHP at a concentration of 200 mg kg-1 revealed eosinophilic droplets in the epithelium of renal tubules and histological examination in fish injected with γ-HCH at a higher concentration revealed an increased activation of sinusoidal cells in the liver, a fibrous thickening of bile duct walls, bile duct hyperplasia and a

  14. Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus fingerlings

    Directory of Open Access Journals (Sweden)

    Shao Wei Zhai

    2014-07-01

    Full Text Available The present study was performed with tilapia (Oreochromis niloticus to evaluate the effects of diet supplementation with grape seed proanthocyanidins (GSPs on fish growth performance, some serum parameters and body composition. Three hundred tilapia fingerlings with the initial average body weight of 9.50±1.25 g were randomly divided into five treatment groups with four replicates in each group and 15 fish in each replicate. The dietary GSPs levels of five treatment groups were 0 (control group, 200, 400, 600, and 800 mg/kg, respectively. The trial period was 49 days. Growth performance parameters were significantly improved by GSPs supplementation (P<0.05, while survival rates were similar among all groups (P>0.05. Serum parameter results showed that activities of aminotransferase aspartate in 200 and 400 mg/kg GSPs groups and alanine aminotransferase in 400 mg/kg GSPs group were lowered significantly (P<0.05. Levels of triglyceride and total cholesterol (except 200 mg/kg GSPs group were significantly lowered, while lysozyme activity and albumin level were significantly higher in fish of GSPs supplemented groups, independently from the level of supplementation. The highest crude protein level and lowest crude lipid level were found in fish of all GSPs supplemented groups, while levels of moisture and ash in fish of all groups were similar (P>0.05. The results indicated that dietary 200 mg/kg GSPs could exert beneficial effects on growth and body composition of tilapia fingerlings, and ameliorate serum biochemistry parameters related to health status.

  15. Effects of Ramadan Fasting on Some Physiological and Biochemical Parameters in Healthy and Hypertensive Subjects in Aurangabad District of Maharashtra, India

    OpenAIRE

    Mohammad Salahuddin; Masood-ul-Hassan Javed

    2014-01-01

    Throughout the world, millions of Muslims fast daily during the month of Ramadan from sunrise (Sahar) until sunset (Iftar). Considering the impacts of environment on physiological functions, we performed this study in order to examine the effects of Ramadan fasting on some blood parameters of healthy volunteers and hypertensive patients. According to the results, there were no significant changes in the weight, heart rate (HR), blood pressure (BP), serum total cholesterol and packed cell volu...

  16. Body Composition, Hemodynamic and Biochemical Parameters in Young Female Normal-Weight Oligo-amenorrheic and Eumenorrheic Athletes and Non-athletes

    Science.gov (United States)

    Singhal, Vibha; de Lourdes Eguiguren, Maria; Eysenbach, Lindsey; Clarke, Hannah; Slattery, Meghan; Eddy, Kamryn; Ackerman, Kathryn E.; Misra, Madhusmita

    2014-01-01

    Aims Low-weight hypogonadal conditions such as anorexia nervosa are associated with marked changes in body composition, hemodynamic and hematological parameters, and liver enzymes. The impact of athletic activity in normal-weight adolescents with/without amenorrhea on these parameters has not been assessed. Our aim was to examine these parameters in normal-weight athletes and non-athletes and determine any associations of body composition, oligo-amenorrhea and exercise intensity. Methods We assessed vital signs, complete blood counts, liver enzymes, and regional body composition in 43 oligo-amenorrheic athletes (OAA), 24 eumenorrheic athletes (EA) and 23 non-athletes 14-21 years of age. Results BMI was lower in OAA than EA. Systolic and pulse pressure, and temperature were lowest in OAA. Blood counts did not differ among groups. AST was higher in both groups of athletes, while ALT was higher in OAA than EA and non-athletes. Total and regional fat was lower in OAA than other groups, positively associated with heart rate and inversely with liver enzymes. Conclusions Athletic activity is associated with higher AST, whereas menstrual dysfunction is associated with lower total and regional fat and higher ALT. Higher liver enzymes are associated with reductions in total and regional fat. PMID:25376841

  17. Studies on intermolecular interaction on binary mixtures of methyl orange-water system: excess molar functions of ultrasonic parameters at different concentrations and at different temperatures.

    Science.gov (United States)

    Thanuja, B; Kanagam, Charles; Sreedevi, S

    2011-11-01

    Density (ρ), viscosity (η) and ultrasonic velocity (u) of binary mixtures of methyl orange and water were measured at different concentrations and at different temperatures; several useful parameters such as excess volume, excess velocity, and excess adiabatic compressibility have been calculated. These parameters are used to explain the nature of intermolecular interactions taking place in the binary mixture. The above study is helpful in understanding the dye/solvent interaction at different concentration and temperatures. PMID:21596612

  18. 辐射传输模型多尺度反演植被理化参数研究进展%Review of inversing biophysical and biochemical vegetation parameters in various spatial scales using radiative transfer models

    Institute of Scientific and Technical Information of China (English)

    肖艳芳; 周德民; 赵文吉

    2013-01-01

    Vegetation is one of the most important components of the earth ecosystem,and its biophysical and biochemical parameters are closely correlated to many exchanges of energy and matter in natural environments.So estimation of the key biophysical and biochemical variables accurately is critical for many ecological,agronomic,and meteorological applications,such as monitoring the growth condition of vegetation,warning the forest fire and studying the global carbon-nitrogen cycle.Empirical-statistical approach and physically based canopy reflectance model are the two main methods to estimate vegetation characteristics.Since the vegetation reflectance is impacted by several internal and external factors,which are different both spatially and temporally according to the different vegetation types.There is a critical issue on the empiricalstatistical approach due to its lacking of generality.And the empirical-statistical approach will be site-,time-and cropspecific because of the existed relationship of reflectance and its established biochemical and biophysical parameters.The vegetation radiative transfer models,as the most important physically based model,are established on fundamental theories of mathematics,physics and biology,and they describe the transfer and interaction of radiation inside the canopy based on physical laws.Many researchers have proved the radiative transfer models as an important tool to understand and quantify the relationship between vegetation object properties and remotely detected radiance signals,with their strong stability and good spatiotemporal transferability by comparing with the empirical-statistical approach.On the basis of different vegetation types and research objectives,a variety of radiative transfer models have been developed for different research purpose in the past two decades,and there are many reports on the application of these models.In this review,we focused on introducing the vegetation radiative transfer models used to

  19. Effects of Ramadan fasting on some physiological and biochemical parameters in healthy and hypertensive subjects in Aurangabad district of Maharashtra, India

    Directory of Open Access Journals (Sweden)

    Mohammad Salahuddin

    2014-02-01

    Full Text Available Throughout the world, millions of Muslims fast daily during the month of Ramadan from sunrise (Sahar until sunset (Iftar. Considering the impacts of environment on physiological functions, we performed this study in order to examine the effects of Ramadan fasting on some blood parameters of healthy volunteers and hypertensive patients. According to the results, there were no significant changes in the weight, heart rate (HR, blood pressure (BP, serum total cholesterol and packed cell volume (PCV of volunteers before and after fasting (P0.05. Therefore, it can be concluded that hypertensive patients, while continuing their previous medications, can safely fast during the month of Ramadan.

  20. Effects of Ramadan Fasting on Some Physiological and Biochemical Parameters in Healthy and Hypertensive Subjects in Aurangabad District of Maharashtra, India

    Directory of Open Access Journals (Sweden)

    Mohammad Salahuddin

    2014-03-01

    Full Text Available Throughout the world, millions of Muslims fast daily during the month of Ramadan from sunrise (Sahar until sunset (Iftar. Considering the impacts of environment on physiological functions, we performed this study in order to examine the effects of Ramadan fasting on some blood parameters of healthy volunteers and hypertensive patients. According to the results, there were no significant changes in the weight, heart rate (HR, blood pressure (BP, serum total cholesterol and packed cell volume (PCV of volunteers before and after fasting (P0.05. Therefore, it can be concluded that hypertensive patients, while continuing their previous medications, can safely fast during the month of Ramadan.

  1. Modelling the anaerobic digestion of solid organic waste - Substrate characterisation method for ADM1 using a combined biochemical and kinetic parameter estimation approach.

    Science.gov (United States)

    Poggio, D; Walker, M; Nimmo, W; Ma, L; Pourkashanian, M

    2016-07-01

    This work proposes a novel and rigorous substrate characterisation methodology to be used with ADM1 to simulate the anaerobic digestion of solid organic waste. The proposed method uses data from both direct substrate analysis and the methane production from laboratory scale anaerobic digestion experiments and involves assessment of four substrate fractionation models. The models partition the organic matter into a mixture of particulate and soluble fractions with the decision on the most suitable model being made on quality of fit between experimental and simulated data and the uncertainty of the calibrated parameters. The method was tested using samples of domestic green and food waste and using experimental data from both short batch tests and longer semi-continuous trials. The results showed that in general an increased fractionation model complexity led to better fit but with increased uncertainty. When using batch test data the most suitable model for green waste included one particulate and one soluble fraction, whereas for food waste two particulate fractions were needed. With richer semi-continuous datasets, the parameter estimation resulted in less uncertainty therefore allowing the description of the substrate with a more complex model. The resulting substrate characterisations and fractionation models obtained from batch test data, for both waste samples, were used to validate the method using semi-continuous experimental data and showed good prediction of methane production, biogas composition, total and volatile solids, ammonia and alkalinity. PMID:27156366

  2. Modelling the anaerobic digestion of solid organic waste - Substrate characterisation method for ADM1 using a combined biochemical and kinetic parameter estimation approach.

    Science.gov (United States)

    Poggio, D; Walker, M; Nimmo, W; Ma, L; Pourkashanian, M

    2016-07-01

    This work proposes a novel and rigorous substrate characterisation methodology to be used with ADM1 to simulate the anaerobic digestion of solid organic waste. The proposed method uses data from both direct substrate analysis and the methane production from laboratory scale anaerobic digestion experiments and involves assessment of four substrate fractionation models. The models partition the organic matter into a mixture of particulate and soluble fractions with the decision on the most suitable model being made on quality of fit between experimental and simulated data and the uncertainty of the calibrated parameters. The method was tested using samples of domestic green and food waste and using experimental data from both short batch tests and longer semi-continuous trials. The results showed that in general an increased fractionation model complexity led to better fit but with increased uncertainty. When using batch test data the most suitable model for green waste included one particulate and one soluble fraction, whereas for food waste two particulate fractions were needed. With richer semi-continuous datasets, the parameter estimation resulted in less uncertainty therefore allowing the description of the substrate with a more complex model. The resulting substrate characterisations and fractionation models obtained from batch test data, for both waste samples, were used to validate the method using semi-continuous experimental data and showed good prediction of methane production, biogas composition, total and volatile solids, ammonia and alkalinity.

  3. Evolution of biochemical parameters in irradiated fishes: Serum proteins and intestinal nucleic acids; Evolucion de parametros bioquimicos en peces irradiados: Proteinas en suero y acidos nucleicos en intestino

    Energy Technology Data Exchange (ETDEWEB)

    Garces, F.; Andres, P.; Davila, C. A.

    1976-07-01

    In sublethal gamma-irradiated C. auratus, a sudden decrease of total serum protein concentration and a preferential descent of the low molecular weight gamma-globulin fraction have been observed. These effects are transient and after different latent periods dependent on doses, normal values are recovered, A temporal failure of a vascular permeability regulation system is probably implied. The DMA depolymerization. observed in the intestine indicates the action of radio-induced DNA degradation mechanisms since this effect is independent on doses. (Author) 29 refs.

  4. 黄芪建中汤对柔道选手血液及生化指标效应之研究%Effects of Huangqi Jianzhong Tang on hematological and biochemical parameters in judo athletes

    Institute of Scientific and Technical Information of China (English)

    苏奕彰; 林健蓉; 陈洸艟; 李铭; 林睿珊; 蔡真真; 周玉; 林昭庚

    2001-01-01

    目的:本研究在探讨黄芪建中汤对柔道选手血球及血清生化值的影响.方法:实验中以新民商工高中组柔道选手为对象,男生16名女生8名,采用分层分组的方法,分为实验组与对照组,分别服用黄芪建中汤与安慰剂.实验期间为五周,分前、后二次测量,检验选手血液中血球及血清生化值.结果以配对t检定、变异数分析法、单纯主要效果比较进行分析.结果:研究结果显示,血液中血球(RBC、Hb、Hct)指数于实验前后有显著降低,血清生化值(GOT、GPT、BUN、CK)于实验前后有显著升高,证实训练对血球及血清生化值有影响.结论:黄芪建中汤对柔道选手血球及血液生化值无显著效果,亦无不良之副作用.%AIM: The purpose of the study was to investigate the effects of Huangqi Jianzhong Tang (HQJZT) on hematological and biochemical parameters in judo athletes. METHODS: Sixteen male and eight female judo athletes in Hsin-Ming senior high school were randomly and stratified divided into control and experimental group, which received placebo and HQJZT respectively during the five-week training program. The measurement of the hematological and biochemical parameters was performed twice, just before and after the training. The data was analyzed with paired-t test and ANOVA. RESULTS: The values of RBC, Hb, and Hct were obvious decreased after intervention, while the value of GOT, GPT, BUN, and CK was elevated. CONCLUSION: The results indicated the hematological and biochemical changes were caused by the physical training but not the effects of HQJZT. The HQJZT had no adverse effects on the judo athletes in our study.

  5. Effects of diets differing in protein source and technical treatment on digestibility, performance and visceral and biochemical parameters of fattening pigs.

    Science.gov (United States)

    Liermann, Wendy; Berk, Andreas; Böschen, Verena; Dänicke, Sven

    2016-01-01

    The aim of the experiment on 100 cross-bred barrows was to compare commercial diets for fattening pigs based on either soya bean meal (SBM) imported from non-European countries with diets based on a mixture of locally produced rape seed meal, distillers' dried grains with solubles and soya beans as main protein sources. In addition, these both types of diets were processed by two different technical feed treatments, i.e. coarse grinding without hydrothermal treatment or fine grinding and pelleting. With only few exceptions, nutrients of the diet without SBM were more digestible (p animals that fed diets without SBM (p animals that received coarsely ground feed (p Animals that fed finely ground and pelleted feed had higher slaughter and relative liver weights and higher blood cholesterol concentrations (p = 0.040). The urea concentrations of blood were lower (p = 0.019) after feeding diets without SBM. In conclusion, SBM imported from non-European countries can be replaced by alternative local protein sources without compromising digestibility or performances of animals. Although fine grinding and thermal treatment particularly seemed to be advantageous for digestibility and performance, the possible risk of development of stomach lesions should be considered. PMID:27032030

  6. Evaluation of growth, biochemical and bioaccumulation parameters in Pelophylax perezi tadpoles, following an in-situ acute exposure to three different effluent ponds from a uranium mine

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Sérgio M., E-mail: s.reis.marques@gmail.com [Departamento de Biologia da Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Chaves, Sandra [Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Edifício ICAT, Campus da FCUL Campo Grande, Lisboa (Portugal); Gonçalves, Fernando [Departamento de Biologia da Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Pereira, Ruth [CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Departamento de Biologia da Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto (Portugal)

    2013-02-15

    Mining activities invariably produce metal contaminated effluents. Depending on factors such as pH and metal concentration the toxicity of the effluent may vary. To assess the effects of three characteristically different effluent ponds from a deactivated uranium mine, with toxicologically relevant data, an in situ exposure with Pelophylax perezi tadpoles, was conducted. Tadpoles were exposed to the three effluent ponds, ranked by increasing order of metals concentrations (REF, M1, M2). Survival, growth, metal accumulation, antioxidant enzymes (catalase, glutathione peroxidase and glutathione reductase) and lipid peroxidation (LPO) were determined in tadpoles. As well, physical and chemical variables of the effluents were measured. Death percentage in the effluents was 3.17 (REF), 9.84 (M1) and 42.86% (M2) and was not coincident with metal accumulation which was highest in tadpoles exposed to M1, while metal contents in M2 tadpoles were quite similar to those recorded in REF tadpoles. However, high mortality in M2 was attributed to the extremely low pH (≈ 3.77). From the three effluents M2 tadpoles had the lowest growth and the antioxidant enzymatic activity was only affected in the case glutathione peroxidase (GPx) with significantly higher activity in M1, being in accordance with the highest accumulation of metals. LPO, usually associated with metal accumulation, had the following pattern M1 > REF > M2. Overall, effluent toxicity in tadpoles exposed to M2 effluent seems to be primarily an effect of pH while in M1 toxicity is mainly owed to high metal concentrations. The effluent acidity seems to reduce metal accumulation probably due to damage in the integument, affecting ion uptake. The results obtained bring a better understanding of the toxicological processes that local P. perezi population is subjected to, mainly in the early life stages. Furthermore this study highlights the influence of pH in the toxicity of metal rich effluents. - Highlights:

  7. Evaluation of growth, biochemical and bioaccumulation parameters in Pelophylax perezi tadpoles, following an in-situ acute exposure to three different effluent ponds from a uranium mine

    International Nuclear Information System (INIS)

    Mining activities invariably produce metal contaminated effluents. Depending on factors such as pH and metal concentration the toxicity of the effluent may vary. To assess the effects of three characteristically different effluent ponds from a deactivated uranium mine, with toxicologically relevant data, an in situ exposure with Pelophylax perezi tadpoles, was conducted. Tadpoles were exposed to the three effluent ponds, ranked by increasing order of metals concentrations (REF, M1, M2). Survival, growth, metal accumulation, antioxidant enzymes (catalase, glutathione peroxidase and glutathione reductase) and lipid peroxidation (LPO) were determined in tadpoles. As well, physical and chemical variables of the effluents were measured. Death percentage in the effluents was 3.17 (REF), 9.84 (M1) and 42.86% (M2) and was not coincident with metal accumulation which was highest in tadpoles exposed to M1, while metal contents in M2 tadpoles were quite similar to those recorded in REF tadpoles. However, high mortality in M2 was attributed to the extremely low pH (≈ 3.77). From the three effluents M2 tadpoles had the lowest growth and the antioxidant enzymatic activity was only affected in the case glutathione peroxidase (GPx) with significantly higher activity in M1, being in accordance with the highest accumulation of metals. LPO, usually associated with metal accumulation, had the following pattern M1 > REF > M2. Overall, effluent toxicity in tadpoles exposed to M2 effluent seems to be primarily an effect of pH while in M1 toxicity is mainly owed to high metal concentrations. The effluent acidity seems to reduce metal accumulation probably due to damage in the integument, affecting ion uptake. The results obtained bring a better understanding of the toxicological processes that local P. perezi population is subjected to, mainly in the early life stages. Furthermore this study highlights the influence of pH in the toxicity of metal rich effluents. - Highlights:

  8. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  9. Interactive effects of elevated CO2 concentration and irrigation on photosynthetic parameters and yield of maize in Northeast China.

    Directory of Open Access Journals (Sweden)

    Fanchao Meng

    Full Text Available Maize is one of the major cultivated crops of China, having a central role in ensuring the food security of the country. There has been a significant increase in studies of maize under interactive effects of elevated CO2 concentration ([CO2] and other factors, yet the interactive effects of elevated [CO2] and increasing precipitation on maize has remained unclear. In this study, a manipulative experiment in Jinzhou, Liaoning province, Northeast China was performed so as to obtain reliable results concerning the later effects. The Open Top Chambers (OTCs experiment was designed to control contrasting [CO2] i.e., 390, 450 and 550 µmol·mol(-1, and the experiment with 15% increasing precipitation levels was also set based on the average monthly precipitation of 5-9 month from 1981 to 2010 and controlled by irrigation. Thus, six treatments, i.e. C550W+15%, C550W0, C450W+15%, C450W0, C390W+15% and C390W0 were included in this study. The results showed that the irrigation under elevated [CO2] levels increased the leaf net photosynthetic rate (Pn and intercellular CO2 concentration (Ci of maize. Similarly, the stomatal conductance (Gs and transpiration rate (Tr decreased with elevated [CO2], but irrigation have a positive effect on increased of them at each [CO2] level, resulting in the water use efficiency (WUE higher in natural precipitation treatment than irrigation treatment at elevated [CO2] levels. Irradiance-response parameters, e.g., maximum net photosynthetic rate (Pnmax and light saturation points (LSP were increased under elevated [CO2] and irrigation, and dark respiration (Rd was increased as well. The growth characteristics, e.g., plant height, leaf area and aboveground biomass were enhanced, resulting in an improved of yield and ear characteristics except axle diameter. The study concluded by reporting that, future elevated [CO2] may favor to maize when coupled with increasing amount of precipitation in Northeast China.

  10. Improvement of biochemical parameters in type 1 diabetic rats after the roots aqueous extract of yacon [Smallanthus sonchifolius (Poepp.& Endl.)] treatment.

    Science.gov (United States)

    Oliveira, Gilberto Ornelas; Braga, Camila Pereira; Fernandes, Ana Angélica Henrique

    2013-09-01

    The aim of this study was to evaluate the effect of yacon (Smallanthus sonchifolius) (Poepp.& Endl.) on clinical parameters under diabetic conditions. The aqueous extract of yacon tuberous roots (YRAE; 0.76 g fructan kg⁻¹ body weight) was prepared at the moment of each administration. Thirty-two male rats were divided into four groups (n=8): control group (C); group that received YRAE (Y); untreated diabetic group (DM1); and diabetic group treated with YRAE (Y-DM1). The diabetes mellitus was induced by streptozotocin (60 mg kg⁻¹ body weight). The animals from Y2 and Y-DM1 received YRAE by gavage, at 7-day intervals, for 30 days. The aqueous extract of yacon roots decreased (p0.05) between the Y and Y-DM1 groups. YRAE normalised alanine aminotransferase (ALT) activity, when comparing DM1 and Y-DM1 rats, but had no effect on lactate dehydrogenase activity (LDH). In conclusion, YRAE was sufficient for controlling water and food consumption, hyperglycaemia and dyslipidaemia, and promote the reduction of the ALT, suggesting a hepatoprotective effect in rats with STZ-induced DM1. PMID:23770327

  11. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  12. Effect of priming on growth, biochemical parameters and mineral composition of different cultivars of coriander (Coriandrum sativum L. under salt stress

    Directory of Open Access Journals (Sweden)

    Ben Fredj Meriem

    2014-08-01

    Full Text Available At Higher Institute of Agriculture of Chott Mariem, Sousse, Tunisia, this study was conducted to evaluate the interactive effect of salinity and seed priming on coriander. The experiment was carried out in completely randomized design with three replications consisting of four coriander genotypes (Tunisian cv, Algerian cv, Syrian cv and Egyptian cv at two seed conditions (seed priming with 4 g/l NaCl for 12h or no seed priming. Results revealed that seed priming and salinity had significantly (p≤0.05 affected all the parameters under study. On the first hand, salinity stress had adversely affected growth, chlorophyll content, mineral composition (K+ and Ca2+ of coriander in all genotypes. Also, it activated Na+ accumulation and synthesis of proline, soluble sugars and proteins. However, seed priming with NaCl had diminished the negative impact of salt stress in all cultivars and primed plants showed better response to salinity compared to unprimed plants. Maximum values were recorded in tolerant cultivar which is Tunisian one whereas minimum values were noted in sensitive cultivar (Algerian cv.

  13. Investigation on the effect of different levels of dried sweet orange (Citrus sinensis) pulp on performance, carcass characteristics and physiological and biochemical parameters in broiler chicken.

    Science.gov (United States)

    Abbasi, Hossein; Seidavi, Alireza; Liu, Wuyi; Asadpour, Leila

    2015-03-01

    Utilization of agricultural by-products in animal nutrition is a matter of great concern. Dried sweet orange (Citrus sinensis) pulp (DCSP) is a potential source of valuable nutrients and natural antioxidants for poultry feed. In the experiment, a feeding trial was conducted in order to investigate the effect of different levels of dried orange residues in diet on broiler growth performance, carcass characteristics, blood metabolites, humoral immunity, and cecum microbial population. A total of 200 one day experimental broiler chicks were distributed into a completely randomized design (CRD) which included 5 dietary treatments with 4 replicates per each treatment and 10 birds fed in each replicate. The experimental treatments consist of a control group (without additive), 0.5%, 1.0%, 1.5%, and 2% of DCSP (residue) in diet. Weight gain, feed intake and feed conversion ratio (FCR) were measured. Blood parameters and carcass traits were measured in the postnatal 35th day. The highest level of dried orange residues in treatment 5 (T5) had significantly increased the feed intake and body weight of broilers in groups and overall during the rearing period (P > 0.05). Different levels of dried orange residues had no significant effect on chicken FCR. Using of dried orange residues significantly decreased the liver and abdominal fat of broilers (P broiler chicken. PMID:25737644

  14. Calculation Model of Mass Action Concentration for Mg-Al, Sr-Al and Ba-Al Melts and Determination of Their Thermodynamic Parameters

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Based on the phase diagrams and the mass action law in combination with the coexistence theory of metallic melts structure, the calculation model of mass action concentration for Mg-Al, Sr-Al and Ba-Al was built, and their thermodynamic parameters were determined. The agreement between calculated and measured results shows that the model and the determined thermodynamic parameters can reflect the structural characteristics of relevant melts. However, the fact that the thermodynamic parameters from literature don′t give the value agree with the measured result may be due to unconformity of these parameters to real chemical reactions in metallic melts.

  15. The Effect of Humic Acid Applications on Some Morphological, Physiological and Biochemical Characteristics of Eggplants Irrigated with Water Contained Heavy Metals in High Concentration

    Directory of Open Access Journals (Sweden)

    Sevinc Kiran

    2014-06-01

    Full Text Available In this study, it was aimed to demonstrate the effect of humic acid applications on some morphological, physiological and biochemical characteristics of eggplant genotypes (Burdur Merkez, Burdur Bucak, Kemer and Giresun irrigated with the irrigation water with a high content of heavy metal and determined previously salt tolerance levels. In studies conducted in controlled greenhouse conditions, eggplant seeds germinated in the growth substrate a mixture of peat and perlite and the seedlings were transplanted into pots at 20 days after sowing. Plants when they are 4-5 true leaves, 3 different humic acid levels (0, 500, 1000 ppm have been applied and 7 days later after this application began to be watered with 3 different irrigation water comprising a mixture of various doses of heavy metals (control: 0 ppm; I. Mixture: 0.2 ppm 5 ppm to 0.01 ppm Cd + Cu + Pb + 2 ppm Zn, II. mixture: +0.02 ppm 0.4 ppm Cu 10 ppm Pb + Cd + 4 ppm Zn. Field capacity level for the plants 40 days after quenched with water after which time they were harvested and samples for analysis were performed. In the study plants were investigated for shoot and root fresh weight, shoot and root dry weight, shoot and root length, leaf area, chlorophyll and MDA level of superoxide dismutase (SOD, catalase (CAT, ascorbate peroxidase (APX and glutathione reductase (GR enzyme activities All genotypes are adversely affected by heavy metal applications. In parallel to increase the dose, heavy metal mixtures led to a reduction in values of fresh and dry weight of shoot and root, stem and root length, leaf area of eggplant genotypes. MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. Humic acid applications had a positive effect on reducing of the limiting effect of heavy metal stress on growth and development. As a result, compared to sensitive genotypes Giresun and Kemer, salt tolerant genotypes Burdur Merkez and Burdur

  16. Haematological and biochemical analysis in canine enteritis

    Directory of Open Access Journals (Sweden)

    Abid Ali Bhat

    Full Text Available Aim: The present investigation screened eighteen clinical cases of canine enteritis for haematological and biochemical analyses. Materials and Methods: Eighteen dogs suffering from enteritis were selected and detailed clinical manifestations were noted. Hematological and biochemical parameters were estimated by using various kits. Blood was also collected from twelve healthy dogs for establishing control values and data obtained were subjected to statistical analysis. Results: The affected dogs showed anorexia, diarrhoea, depression, varying degree of dehydration and tachycardia. There were significant changes in packed cell volume, neutrophils, lymphocytes and mean corpuscular haemoglobin concentration. Biochemical investigation revealed significant decrease in plasma glucose, total plasma protein, albumin and albumin:globulin ratio (A:G ratio. The level of potassium and chloride was markedly decreased. Significant increase in alanine aminotransferase (ALT and blood urea nitrogen (BUN was observed. Conclusion: Packed Cell Volume (PCV and Total Erythrocyte Count (TEC remained almost similar between healthy dogs and dogs affected with diarrhoea. Mean Total Leukocyte Count (TLC value was significantly higher as compared to the control group. Hypoglycemia, hypoproteinemia, hypokalemia, hypochloremia and increase in blood urea nitrogen was observed in dogs suffering from enteritis. [Vet World 2013; 6(7.000: 380-383

  17. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: effects on hemolymph and biochemical parameters.

    Science.gov (United States)

    An, Myung In; Choi, Cheol Young

    2010-01-01

    Changes in water temperature and salinity are responsible for a variety of physiological stress responses in aquatic organisms. Stress induced by these factors was recently associated with enhanced reactive oxygen species (ROS) generation, which caused oxidative damage. In the present study, we investigated the time-related effects of changes in water temperature and salinity on mRNA expression and the activities of antioxidant enzymes (SOD and CAT) and lipid peroxidation (LPO) in the gills and digestive glands of the ark shell, Scapharca broughtonii. To investigate physiological responses, hydrogen peroxide (H(2)O(2)), lysozyme activity, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT) were measured in the hemolymph. Water temperature and salinity changes significantly increased antioxidant enzyme mRNA expression and activity in the digestive glands and gills in a time-dependent manner. H(2)O(2) concentrations increased significantly in the high-temperature and hyposalinity treatments. LPO, AspAT and AlaAT levels also increased significantly in a time-dependent manner, while lysozyme activity decreased. These results suggest that antioxidant enzymes play important roles in reducing oxidative stress in ark shells exposed to changes in water temperature and salinity.

  18. Trans fat intake across gestation and lactation increases morphine preference in females but not in male rats: Behavioral and biochemical parameters.

    Science.gov (United States)

    Roversi, Karine; Pase, Camila Simonetti; Roversi, Katiane; Vey, Luciana Taschetto; Dias, Verônica Tironi; Metz, Vinícia Garzella; Burger, Marilise Escobar

    2016-10-01

    The abuse of morphine has risen considerably in recent years, mainly due to the increase of its prescription in clinical medicine. Also, increased consumption of processed foods, rich in trans fatty acids (TFA), has caused concerns about human health. Thus, the aim of our study was to determine whether trans fat consumption in the perinatal period may affect preference for morphine in adolescent female and male rats. Dams were orally supplemented with water (C-control) or hydrogenated vegetable fat (HVF-rich in TFA) during gestation and lactation periods. On post-natal day 43, pups were exposed to morphine (4mg/kg i.p., for 4 days) and assessed in the conditioned place preference paradigm. Anxiety-like symptoms were assessed, and oxidative status of the brain was estimated by reactive species (RS) generation. Female rats with HVF supplementation showed increased morphine preference and less anxiety-like symptoms. Additionally, both male and female rats from HVF-supplementation showed increased RS generation in the ventral tegmental area, whose level was similar in morphine-conditioned female rats. RS generation was increased in the hippocampus of morphine-conditioned female rats, regardless of the supplementation of their dams. We may infer that gender is a predictive factor to opioid preference, since adolescent female rats showed more susceptibility to addiction than males. Furthermore, trans fat consumption across the perinatal period is able to modify parameters of opioid preference in female rats, possibly due to TFA incorporation in phospholipid membranes, modifying the endogenous opioid system and the oxidative status in brain areas related to drug addiction.

  19. The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Siti Balkis Budin

    2009-03-01

    Full Text Available OBJECTIVE: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats. METHODS: Animals were divided into three groups: (i normal non-diabetic (NDM, (ii diabetic treated (tocotrienol-rich fractions - TRF and (iii diabetic untreated (non-TRF. The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated. RESULTS: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall. CONCLUSION: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

  20. Effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of Blue-and-gold macaws (Ara ararauna L. - Aves, Psittacidae).

    Science.gov (United States)

    Veloso, R R; Sakomura, N K; Kawauchi, I M; Malheiros, E B; Carciofi, A C

    2014-04-01

    Considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the Blue-and-gold macaw. Four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. To study digestibility, 48 macaws housed in pairs in cages was used in a block design. Subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months to evaluate energy intake and blood metabolites. A 2 × 2 × 2 (two fibre levels, two food processing methods and two genders) factorial arrangement with subplots (beginning and end) was used. When differences were detected in anova's F test, data were submitted to polynomial contrasts in the first experiment and to orthogonal contrasts in the second experiment (p food processing. Pelleted foods presented higher dry matter digestibility and food metabolisable energy (ME) than the extruded ones (p processing did not change ME ingestion (p > 0.05). The macaws gained body weight (p 0.05), but females fed with the high-fibre diets did not gain weight (p > 0.05), suggesting a low food ME (12.5 kJ/g).The substitution of the original diet (sunflower seeds, fruits and cooked maize) by the experimental foods decreased the basal (12-h fast) concentrations of glucose, cholesterol and triglycerides (p < 0.001). The consumption of pelleted diets reduced serum glucose and cholesterol (p < 0.05). Results suggest that the pelleted diets were more beneficial and can be used to reduce blood metabolites related to metabolic disorders that are commonly observed in macaws.

  1. [Effect of stress actions on some hematologic and biochemical parameters of rat blood and on energetic intermolecular interactions in lipid extract under effect of light radiation].

    Science.gov (United States)

    Zabelinskiĭ, S A; Chebotareva, M A; Tavrovskaia, T V; Skverchinskaia, E A; Shukoliukova, E P; Maslov, M N; Krivchenko, A I

    2012-01-01

    Comparative study has been carried of effect of the three-day long starvation, running, and their combination on morphological parameters of rat blood, lipid metabolism, and activity of blood Na,K-ATPase. Different effect has been shown of these stress factors on the blood erythrocyte composition. Starvation is accompanied by the most pronounced release of stored erythrocyte into blood, which results in a significant decrease both of the total amount of reticulocytes and the complete absence of reticulocytes of the I stage of maturity (the youngest). The running on treadmill led to a significant increase of the total amount of blood reticulocytes and to multiple increase of immature reticulocytes (RC-I and RC-II), which can indicate some stress of the bone marrow erythroid stem line. The curve of acid resistance of blood reticulocytes has shown the animal to experience the greatest stress at a combination of starvation and running. Starvation and running produced different effects on blood lipid characteristics. The content of triacylglycerides (TAG) in blood rose by 40% at starvation and decreased by 30% at running, a similar tendency being found for index of atherogeneity. The fatty acid composition of blood phospholipids at running and its combination with starvation practically did not differ from control. A change of Na,K-ATPase, which is so characteristic of reaction to various kinds of stress, sharply fell at starvation (by 22%), but increased at running (by 13%) and decreased markedly at combination of these actions. Absorption spectra of lipid extracts of the whole blood of the rats submitted to various stress actions showed that extracted from blood (at different amount depending on the kind of action) is an organic substance with coupled bonds, which absorbs light in the diapason of 360-620 nm. The absorption of light in the diapason of 400-410 nm has been found to belong to the Soret band of ferroheme and ferriheme. The shift of the Soret band indicates

  2. Effect of fishmeal replacement by soy protein concentrate with taurine supplementation on growth performance, hematological and biochemical status, and liver histology of totoaba juveniles (Totoaba macdonaldi).

    Science.gov (United States)

    López, Lus M; Flores-Ibarra, Maricela; Bañuelos-Vargas, Isaura; Galaviz, Mario A; True, Conal D

    2015-08-01

    The effect of dietary inclusion of soy protein concentrate (SPC) and simultaneous supplementation with taurine on the growth, hematology, blood biochemistry, and liver histology of totoaba (Totoaba macdonaldi) juveniles was assessed. Four isoproteic and isolipidic diets were formulated containing either 30 or 60% of SPC (diets S30 and S60), supplemented or not with 1% of taurine (diets S30T and S60T). A fishmeal-based diet formulated for totoaba nutritional requirements, without SPC and taurine supplementation, was used as a reference diet. Triplicate groups of 32 totoaba juveniles (average body weight 7.5 ± 0.6 g) were fed these diets for 45 days. Results showed that growth performance in fish fed S30, S30T, and S60T was similar to fish fed the reference diet. Red blood cells and hematocrit in fish fed with supplemented taurine in both levels of SPC (S30T and S60T) were similar to the fish fed the RD; the addition of taurine improved the state of hydration of totoaba. Plasmatic hemoglobin in fish fed the lower SPC level was similar to fish fed the RD. The mean corpuscular hemoglobin concentration in fish fed S30T was similar to fish fed the RD, taurine supplementation prevented the development of hypochromic anemia in this group of fish. Plasmatic albumin in fish fed S30 was similar to fish fed the RD. Plasmatic total protein and globulin concentration increased and AL:GLB (albumin:globulin ratio) decreased in fish fed the SPC-based diets despite taurine supplementation. The protein profile showed that taurine supplementation did not prevent a possible inflammatory process (increased globulins, decreased AL:GLB) in juvenile totoaba fed both levels of SPC. Glucose concentration was similar in fish fed S30, S30T, and S60T. The histological hepatic index was highest in fish fed S60. These results suggest that with an appropriate nutritional level, taurine may play an important modulatory role in the hematology and blood biochemistry status in totoaba fed SPC

  3. The activity concentrations of 222Rn and corresponding health risk in groundwater samples from basement and sandstone aquifer; the correlation to physicochemical parameters

    Science.gov (United States)

    Abdurabu, Wedad Ali; Ramli, Ahmad Termizi; Saleh, Muneer Aziz; Heryansyah, Arien

    2016-10-01

    This study aims to evaluate the activity concentrations of 222Rn and to assess the corresponding health risk in groundwater samples obtained in Juban District, Ad Dali' Governorate, Yemen. The measurements were performed by RAD 7 radon detector manufactured by DURRIDGE COMPANY Inc. The activity concentrations of 222Rn ranged from 1.0±0.2 Bq l-1 to 896.0±0.8 Bq l-1. 57% of the groundwater samples were above the US Environmental Protection Agency (USEPA) recommended value for Rn in water. Induced coupled plasma mass spectrometry (ICP-MS) was used to determine the concentrations of uranium in groundwater samples. The measured concentration of U ranged from 0.33±0.01 μg l-1 to 24.6±0.6 μg l-1. The results were comparable to internationally recommended values. The highest concentration of U and 222Rn were found to be in the basement aquifer, while the lowest concentrations of both radionuclides were in the sandstone aquifer. High concentrations of Rn are found along fault zones. The relationship between the activity concentration of 222Rn, concentration of U and physicochemical parameters were investigated. The results showed a very strong relationship between activity concentrations of 222Rn with concentrations of U and the salinity of water.

  4. Determination of negligible concentration threshold values for water pollution assessment; Ableitung von Geringfuegigkeitsschwellen zur Beurteilung von Grundwasserverunreinigungen. T. 2. Organische Parameter

    Energy Technology Data Exchange (ETDEWEB)

    Trenck, K.T. von der [Landesanstalt fuer Umweltschutz Baden-Wuerttemberg, Karlsruhe (Germany); Roeder, R.; Slama, H. [Bayerische Landesanstalt fuer Wasserforschung (BayLWF), Muenchen (Germany); Markard, C.; Kuehl, C. [Umweltbundesamt, Berlin (Germany)

    1999-12-01

    Part I of the status report contains the derivation criteria, the reasons for the inorganic parameters chosen, and explanations of terms and abbreviations. A table is presented which contains negligible concentration threshold values for selected parameters of inorganic and organic substances. [German] Der erste Teil des Statusberichts enthaelt eine ausfuehrliche Darstellung der Ableitungskriterien sowie die Begruendung fuer die anorganischen Parameter und Erlaeuterungen zu den Abkuerzungen und Begriffen. Entsprechende Konzentrationswerte fuer die Geringfuegigkeitsschwellen einer Auswahl an Parametern enthaelt eine in der vorliegenden Arbeit aufgefuehrte Tabelle getrennt nach anorganischen und organischen Stoffen. (orig.)

  5. Noise and critical phenomena in biochemical signaling cycles at small molecule numbers

    Science.gov (United States)

    Metzner, C.; Sajitz-Hermstein, M.; Schmidberger, M.; Fabry, B.

    2009-08-01

    Biochemical reaction networks in living cells usually involve reversible covalent modification of signaling molecules, such as protein phosphorylation. Under conditions of small molecule numbers, as is frequently the case in living cells, mass-action theory fails to describe the dynamics of such systems. Instead, the biochemical reactions must be treated as stochastic processes that intrinsically generate concentration fluctuations of the chemicals. We investigate the stochastic reaction kinetics of covalent modification cycles (CMCs) by analytical modeling and numerically exact Monte Carlo simulation of the temporally fluctuating concentration. Depending on the parameter regime, we find for the probability density of the concentration qualitatively distinct classes of distribution functions including power-law distributions with a fractional and tunable exponent. These findings challenge the traditional view of biochemical control networks as deterministic computational systems and suggest that CMCs in cells can function as versatile and tunable noise generators.

  6. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits. PMID:27777945

  7. The influence of soil organic carbon on interactions between microbial parameters and metal concentrations at a long-term contaminated site

    Energy Technology Data Exchange (ETDEWEB)

    Muhlbachova, G. [Crop Research Institute, Drnovska 507, 161 06 Prague 6, Ruzyne (Czech Republic); Sagova-Mareckova, M., E-mail: sagova@vurv.cz [Crop Research Institute, Drnovska 507, 161 06 Prague 6, Ruzyne (Czech Republic); Omelka, M. [Charles University, Faculty of Mathematics and Physics, Dept. of Probability and Mathematical Statistics, Prague 8, Karlin (Czech Republic); Szakova, J.; Tlustos, P. [Czech University of Life Sciences, Department of Agroenvironmental Chemistry and Plant Nutrition, Prague 6, Suchdol (Czech Republic)

    2015-01-01

    The effects of lead, zinc, cadmium, arsenic and copper deposits on soil microbial parameters were investigated at a site exposed to contamination for over 200 years. Soil samples were collected in triplicates at 121 sites differing in contamination and soil organic carbon (SOC). Microbial biomass, respiration, dehydrogenase activity and metabolic quotient were determined and correlated with total and extractable metal concentrations in soil. The goal was to analyze complex interactions between toxic metals and microbial parameters by assessing the effect of soil organic carbon in the relationships. The effect of SOC was significant in all interactions and changed the correlations between microbial parameters and metal fractions from negative to positive. In some cases, the effect of SOC was combined with that of clay and soil pH. In the final analysis, dehydrogenase activity was negatively correlated to total metal concentrations and acetic acid extractable metals, respiration and metabolic quotient were to ammonium nitrate extractable metals. Dehydrogenase activity was the most sensitive microbial parameter correlating most frequently with contamination. Total and extractable zinc was most often correlated with microbial parameters. The large data set enabled robust explanation of discrepancies in organic matter functioning occurring frequently in analyzing of contaminated soil processes. - Highlights: • Soil organic carbon affected all interactions between metals and microorganisms. • Soil organic carbon adjustment changed correlations from positive to negative. • Ammonium nitrate extractable metals were the most influencing fraction. • Dehydrogenase activity was the most affected soil parameter. • Zinc was the most toxic metal among studied metals.

  8. Dark-ages Reionization & Galaxy Formation Simulation II: Spin and concentration parameters for dark matter haloes during the Epoch of Reionization

    CERN Document Server

    Angel, Paul W; Ludlow, Aaron D; Duffy, Alan R; Geil, Paul M; Mutch, Simon J; Mesinger, Andrei; Wyithe, J Stuart B

    2015-01-01

    We use high resolution N-Body simulations to study the concentration and spin parameters of dark matter haloes in the mass range $10^8\\, {\\rm M}_{\\odot}\\, h^{-1} }5$ concentration$-$mass ($c(M)$) relation that is almost flat and well described by a simple power-law for both NFW and Einasto fits. The intrinsic scatter around the mean relation is $\\Delta c_{\\rm{vir}}{\\sim1}$ (or 20 per cent) at $z=5$. We also find that the analytic model proposed by Ludlow et al. reproduces the mass and redshift-dependence of halo concentrations. Our best-fit Einasto shape parameter, $\\alpha$, depends on peak height, $\

  9. Introducing enzyme selectivity as a quantitative parameter to describe the effects of substrate concentration on protein hydrolysis

    NARCIS (Netherlands)

    Butré, C.I.

    2014-01-01

    To understand the differences in peptide composition that result from variations in the conditions of enzymatic hydrolysis of proteins (e.g. substrate concentration) the mechanism of hydrolysis needs to be understood in detail. Therefore, methods and tools were developed to characterize and quantify

  10. Effects of oxygen concentration and body weight on maximum feed intake, growth and hematological parameters of Nile tilapia, Oreochromis niloticus

    NARCIS (Netherlands)

    Tran Duy, A.; Schrama, J.W.; Dam, van A.A.; Verreth, J.A.J.

    2008-01-01

    Feed intake and satiation in fish are regulated by a number of factors, of which dissolved oxygen concentration (DO) is important. Since fish take up oxygen through the limited gill surface area, all processes that need energy, including food processing, depend on their maximum oxygen uptake capacit

  11. Dark-ages reionization and galaxy formation simulation - II. Spin and concentration parameters for dark matter haloes during the epoch of reionization

    Science.gov (United States)

    Angel, Paul W.; Poole, Gregory B.; Ludlow, Aaron D.; Duffy, Alan R.; Geil, Paul M.; Mutch, Simon J.; Mesinger, Andrei; Wyithe, J. Stuart B.

    2016-06-01

    We use high-resolution N-body simulations to study the concentration and spin parameters of dark matter haloes in the mass range 108 M⊙ h-1 population that also includes unrelaxed systems. Concentrations are calculated by fitting both NFW and Einasto profiles to the spherically averaged density profiles of individual haloes. After removing haloes that are out of equilibrium, we find a z > 5 concentration-mass (c(M)) relation that is almost flat and well described by a simple power law for both NFW and Einasto fits. The intrinsic scatter around the mean relation is Δcvir ˜ 1 (or 20 per cent) at z = 5. We also find that the analytic model proposed by Ludlow et al. reproduces the mass and redshift dependence of halo concentrations. Our best-fitting Einasto shape parameter, α, depends on peak height, ν, in a manner that is accurately described by α = 0.0070ν2 + 0.1839. The distribution of the spin parameter, λ, has a weak dependence on equilibrium state; λ peaks at roughly ˜0.033 for our relaxed sample, and at ˜0.04 for the full population. The spin-virial mass relation has a mild negative correlation at high redshift.

  12. Age-Related Reference Intervals of the Main Biochemical and Hematological Parameters in C57BL/6J, 129SV/EV and C3H/HeJ Mouse Strains.

    Directory of Open Access Journals (Sweden)

    Cristina Mazzaccara

    Full Text Available BACKGROUND: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. METHODOLOGY/PRINCIPAL FINDINGS: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5(th and 97.5(th percentiles. The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001. Inter-strain differences were observed for: (1 GLU, t-Bil, K+, Ca++, PO(4- (p<0.05 and for TAG, Chol, AST, Fe++ (p<0.001 in 4-8 month-old animals; (2 for CK, Crea, Mg++, Na++, K+, Cl- (p<0.05 and BUN (p<0.001 in 2- and in 10-12 month-old mice; and (3 for WBC, RBC, HGB, HCT and PLT (p<0.05 during the 1 year life span. CONCLUSION/SIGNIFICANCE: Our results indicate that metabolic variations in C57BL/6J, 129SV/EV and C3H/HeJ mice after therapeutic intervention should be evaluated against gender- and age-dependent reference intervals.

  13. METABOLIC PARAMETERS CONCENTRATIONS IN BLOOD SERUM OF CZECH PIED BULLS DEPENDING ON SINGLE NUCLEOTIDE POLYMORPHISM OF LEPTIN GENE

    Directory of Open Access Journals (Sweden)

    Aleš Pavlík

    2014-02-01

    Full Text Available The aim of present study was to test hypothesis, that the leptin gene single nucleotide polymorphism (C/T giving missense mutation (Arg25Cys has an effect on concentration of blood serum total cholesterol, beta-hydroxybutyrate and urea in cattle. The experiment were performed in 58 Czech Pied bulls at 240 ± 9 days of age, which were divided in three experimental groups depending on different leptin genotypes (CC, n=28; CT, n=21; TT, n=9. Resulting genotypes in the exon 2 were CC (48.3%, CT (36.2%, and TT (15.5%. There were no differences in serum total cholesterol, urea, beta-hydroxybutyrate concentrations among the genotypes. Based on our results we may assume that analysed SNP of leptin gene have no effect on nutritional status and energy balance in fattened cattle.

  14. Plasma Concentration of Ascorbic Acid and Some Hematological Parameters in Tobacco Snuffers Among the Igbos of Southeastern Nigeria

    OpenAIRE

    Ureme, SO; Njoku, OU; Ejezie, FE; Ibeh, BO; Ikekpazu, E; Mba, MJ

    2012-01-01

    Background: Tobacco snuffing, like cigarette smoking, is known to be a common habit among the adults of Igbo communities in Nigeria. In view of the various pharmacological actions of nicotine and other additive constituents of tobacco snuff, there is growing concern that ascorbic acid, which is a vital antioxidant, and blood cell production or morphology may be affected. Objective: To investigate the possible effects of prolonged use of tobacco snuff on plasma ascorbic acid concentrations and...

  15. 三种复方中草药对斑点叉尾鮰部分生化指标的影响%Effects of Three Compound Chinese Herbal Medicines on Some Biochemical Parameters in Ictalurus punctatus

    Institute of Scientific and Technical Information of China (English)

    郭永军; 乔之怡; 胡玉花; 陈成勋; 白东清; 丁莉莉; 刘志伟; 邢克智

    2012-01-01

    In order to test the effects of compound Chinese herbal medicine "Yuhuan", "Gantaile" and "Sanhuangfen" on biochemical parameters in Ictalurus punctatus, an oral administration trial was performed. The medicines mentioned above were added to a commercial feed at 5 g/kg separately, and were administrated to the tested fish. The fish was sampled after a continuous 7-day feeding. The biochemical parameters in blood and tissues were assayed. The results show that all the medicines enhanced SOD/MDA and CAT/MDA ratio in serum and spleen. So the antioxidant capacity in serum and spleen was enhanced. All the medicines reduced the antioxidant capacity in head kidney by means of reducing SOD/MDA and CAT/MDA ratio. The "Gantaile" and "Yuhuan" enhanced the antioxidant capacity in gill, while "Sanhuangfen" and "Yuhuan" enhanced the antioxidant capacity in muscle. All the medicines reduced AST and ALT activities in hepato-pancreas. The LZM activity was significantly cut down in hepato-pancreas by "Gantaile" and in head kidney by "Yuhuan", while "Sanhuangfen" boosted LZM activity in muscle significantly. All the medicines enhanced blood leucocytes phagocytosis and reduced AST and ALT activities in hepato-pancreas.%为研究复方中草药“鱼欢”、“肝泰乐”、“三黄粉”对斑点叉尾鮰生化指标的影响,将以上3种中草药按5g/kg饵料的比例加到饵料中,连续喂食斑点叉尾鮰7d后取样,测定血液和组织中的生化指标。结果表明:3种复方中草药均能提高血清和脾脏的SOD/MDA和cAz孙佃A比值,提高血清和脾脏的抗氧化能力;3种复方中草药均能降低头肾的SOD/MDA和CAT/MDA比值,降低其抗氧化能力。“肝泰乐”和“鱼欢”能提高鮰的抗氧化能力,“三黄粉”和“鱼欢”能提高肌肉的抗氧化能力。3种复方中草药能降低肝胰脏中AST和ALT的活力。“肝泰乐”显著降低肝胰脏的LZM活力,“鱼欢”显著降低头肾的LZM活力

  16. Ⅰ型、Ⅱ型酮病对奶牛血液某些理化参数的影响%Effect of typeⅠand type Ⅱ ketosis on Some Blood Biochemical Parameters of Dairy Cow

    Institute of Scientific and Technical Information of China (English)

    李影; 孙玲伟; 张洪友; 夏成; 徐闯; 高阳; 钱伟东

    2014-01-01

    本试验旨在了解Ⅰ型、Ⅱ型酮病发生时奶牛体内某些理化参数的变化。在某集约化牛场随机选取产后7~28 d、平均胎次为2~3胎的试验奶牛,根据血浆中β-羟丁酸(BHBA)、葡萄糖(Glc)、游离脂肪酸(NEFA)的含量以及临床发病特点分为Ⅰ型酮病组(20头)、Ⅱ型酮病组(20头)和健康对照组(10头)。结果显示,三组间血浆中Glc、BH-BA、NEFA的浓度与体况评分存在显著差异(P<0.05),但三组试验奶牛的泌乳量、产后天数、血浆中胰岛素(INS)含量彼此间无显著性差异(P>0.05)。Ⅰ型酮病、Ⅱ型酮病与健康对照组之间的理化参数存在显著差异,为深入探究奶牛Ⅰ型酮病与Ⅱ型酮临床病理学、早期检测和诊断以及详细的发病机理提供依据,为今后有效地预防奶牛Ⅰ型、Ⅱ型酮病奠定基础。%The objective is to investigate some biochemical parameters changes in type I and typeⅡ ketosis of dairy cows. The experimental cows with two or three parities were selected at 7~28 days postpartum in a intensive dairy farm. Cows were classified as type I ketotic (K1, 20 cows), type Ⅱ ketotic (K2, 20 cows), and healthy control group (C, 10 cows) based on the glucose (Glc) and 3-hydroxybutyrate acid (BHBA) levels and the clinical signs. The results revealed that body condition scores, Glc, BHBA and NEFA plasma levels were significantly different among three groups (P0.05). Conclusion that some biochemical parameters were significantly different among type I ketosis, type Ⅱketosis and healthy control cows, thereby providing impor-tant information on the pathogenesis, early diagnosis, and prevention of type I and type Ⅱ keto-sis in dairy cows.

  17. The changes of hematology and biochemical parameters in the beef cattle with subacute ruminal acidosis%肉牛亚急性瘤胃酸中毒血液学及生化指标变化分析

    Institute of Scientific and Technical Information of China (English)

    赵晨旭; 王晓旭; 孙国权; 张良; 陈灰; 杨文涛; 刘兆喜; 王哲; 刘国文

    2013-01-01

    为了解肉牛发生亚急性瘤胃酸中毒时血液学和相应生化指标的变化,对瘤胃液pH在5.2~5.5、5.6~5.8和5.9~6.8之间的24头肉牛进行了血液学及相应生化指标的分析.结果表明:随着pH值的降低,血液中红细胞(RBC)数量、血红蛋白(Hb)浓度、红细胞压积(PCV)均显著增加;白细胞(WBC)总数上升,白细胞分类(WBC-DC)也有相应的变化,嗜酸性粒细胞和淋巴细胞呈下降趋势,中性杆状核粒细胞和中性分叶核粒细胞呈上升趋势,单核细胞略微升高;血气指标pO2显著增加,pCO2、HCO3-浓度显著减少;生化指标Ca2+、Cl-显著减少,Na+和尿素氮显著增加,K+差异不显著.肉牛发生轻度和中度亚急性瘤胃酸中毒时,pCO2以及HCO3-含量显著降低,表明机体碱储备不足,缓冲能力下降,存在代偿性酸中毒;同时根据水合状态和血象指标,表明亚急性瘤胃酸中毒病牛存在轻度脱水和炎性反应.%The aim of this study was to investigate the changes of hematology and biochemical parameters in beef cattle with subacute ruminal acidosis. We analyzed the hematology and biochemical parameters of 24 beef cattle with ruminal pH value in 5. 2-5. 5,5. 6-5. 8 and 5. 9-6. 8. The results showed that red blood cell (RBC), haemoglobin (Hb), packed cell volume(PCV)significant-ly increased with the reducing pH value, the number of white blood celKWBOwas increased. The leukocyte differential count was changed in the different degree, the proportion of eosinophilic leukocyte and lymphocyte was decreased,neutrophilic stab and segmented granulocyte was increased, mononuclear leucocyte was increased slightly. The pO2 was significantly increased. The pCO2 and HCO3- were significantly decreased. The levels of Ca2+ and Cl- were significantly decreased, the levels of Na+ and urea nitrogen were significantly increased,and there was no significant difference in the levels of K+. The decreased pCO2 and HCO3- in the slight and moderate

  18. 针刺对运动性疲劳大鼠部分生化指标的影响%Influence of Acupuncture on Biochemical Parameters in Exercise-induced Fatigue Rats

    Institute of Scientific and Technical Information of China (English)

    李虹霖; 孙远征; 丰晓溟

    2012-01-01

    目的:观察针刺对运动性疲劳大鼠部分生化指标的影响,探讨针刺抗运动性疲劳的作用机制.方法:采用4星期递增游泳训练的方法复制慢性运动性疲劳大鼠模型,随机分为对照组、模型组、头穴组、体穴组,采用“逆针灸”的方法.观察治疗前后各组大鼠力竭游泳时间、全血乳酸(Lactic Acid,LA)、肌酸激酶(Creatine Kinase,CK)、血尿素氮(Blood Urea Nitrogen,BUN)、血红蛋白(Hemoglobin,Hb)及游离色氨酸/支链氨基酸(free Tryptophan/Branched Chain Amino Acid,F-TRP/BCAA)的变化.结果:与体穴组相比,头穴组在提高Hb水平,降低BUN、LA水平方面有明显优势(P<0.05).在降低CK、F-TRP/BCAA水平方面,更是明显优于体穴组(p<0.01).结论:头穴针刺能够有效改善运动性疲劳大鼠生化指标,同时降低F-TRP/BCAA水平,抑制5-HT生成,从而有效地改善大鼠疲劳程度.%Objective:To observe the effect of acupuncture on some biochemical parameters in rats with exercise-induced fatigue and to explore the mechanism of acupuncture for exercise fatigue.Methods:The rat model of chronic exercise-induced fatigue was established by a 4-week increasing swimming training method.The rats were randomly divided into a control group,a model group,a scalp acupoint group,and a body acupoint group.Preventive acupuncture methods were used.Changes of exhaustive swimming time,whole blood lactic acid (LA),creatine kinase (CK),blood urea nitrogen (BUN),hemoglobin (Hb) and free tryptophan/branched chain amino acid (F-TRP/BCAA) of rats in each group before and after treatments were observed.Results:Compared with the body acupoint group,there were obvious advantages of the scalp point group in increasing Hb levels and lowering BUN and LA levels (P<0.05),as well as in aspects of lowering CK and F-TRP/BCAA levels (P<0.01).Conclusion:Scalp point acupuncture can improve the biochemical parameters in rats with exercise-induced fatigue,meanwhile,decrease the F

  19. The Effect of Apium graveolens hydroalcoholic Seed Extract on Sperm Parameters and Serum Testosterone Concentration in Mice

    Directory of Open Access Journals (Sweden)

    P Kerishchi Khiabani

    2014-10-01

    Full Text Available Backgrounds & aim: apium graveolens contains antioxidant activity and high level of polyphenolics. The purpose of this study was to determaine the effect of Apium graveolens seeds extract on semen parameters and serum testosterone level in mice. Methods: In the present experimental study, sixty male mice were divided into three experimental groups and a control group. The hydroalcoholic seed extract of Apium graveolenas L. was administered intraperitoneally at the doses of 200, 400 and 800 mg/kg for 14 days. A week after the final injection, blood samples were collected for hormonal assay. Then, the testes weight, sperm count and cauda epididymal sperm motility was assessed. Data were analyzed by ANOVA and Tukey's test. Results: The results were compared with the control group indicating a significant increase in the total number of sperm at dose 400 mg.kg and increase sperm motility was seen in groups receiving 200 and 400 mg.kg respectively (P<0.001. Increased testosterone levels in the group receiving 400 mg.kg compared with the control group was observed (P<0.01. A significant increase was seen in testes weight compared with the control group (P<0.05. Conclusion: Apium graveolens seed extract appeared to be effective in improving semen parameters and serum total testosterones were dose dependent.

  20. Quantification of Kinetic Rate Law Parameters of Uranium Release from Sodium Autunite as a Function of Aqueous Bicarbonate Concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Gudavalli, Ravi; Katsenovich, Yelena; Wellman, Dawn M.; Lagos, Leonel; Tansel, Berrin

    2013-09-05

    ABSTRACT: Hydrogen carbonate is one of the most significant components within the uranium geochemical cycle. In aqueous solutions, hydrogen carbonate forms strong complexes with uranium. As such, aqueous bicarbonate may significantly increase the rate of uranium release from uranium minerals. Quantifying the relationship of aqueous hydrogen carbonate solutions to the rate of uranium release during dissolution is critical to understanding the long-term fate of uranium within the environment. Single-pass flow-through (SPTF) experiments were conducted to estimate the rate of uranium release from Na meta-autunite as a function of bicarbonate solutions (0.0005-0.003 M) under the pH range of 6-11 and temperatures of 5-60oC. Consistent with the results of previous investigation, the rate of uranium release from sodium autunite exhibited minimal dependency on temperature; but were strongly dependent on pH and increasing concentrations of bicarbonate solutions. Most notably at pH 7, the rate of uranium release exhibited 370 fold increases relative to the rate of uranium release in the absence of bicarbonate. However, the effect of increasing concentrations of bicarbonate solutions on the release of uranium was significantly less under higher pH conditions. It is postulated that at high pH values, surface sites are saturated with carbonate, thus the addition of more bicarbonate would have less effect on uranium release. Results indicate the activation energies were unaffected by temperature and bicarbonate concentration variations, but were strongly dependent on pH conditions. As pH increased from 6 to 11, activation energy values were observed to decrease from 29.94 kJ mol-1 to 13.07 kJ mol-1. The calculated activation energies suggest a surface controlled dissolution mechanism.

  1. Application of the MOS-C-V technique to determine impurity concentrations and surface parameters on the diffused face of silicon solar cells

    Science.gov (United States)

    Weinberg, I.

    1975-01-01

    The feasibility of using the MOS C-V technique to obtain information regarding impurity and surface state concentrations on the diffused face of silicon solar cells with Ta2O5 coatings is studied. Results indicate that the MOS C-V technique yields useful information concerning surface parameters which contribute to the high, efficiency limiting, surface recombination velocities on the n+ surface of silicon solar cells.

  2. Physico-Chemical parameters and trace-metals concentration in effluents from various industries in vicinity of Lahore

    International Nuclear Information System (INIS)

    Increasing problem of pollution has become serious in almost all big cities of Pakistan. The industrial effluents (Liquid waste) discharged by different industries are drained into streams/nallahs, which ultimately join the waterways (streams, lakes, rivers or sea). The effluent samples from five industries, like Tanneries, Chemicals, Pharmaceuticals, Fertilizers and metal/electroplating, working in Lahore, Sheikhupura and Kalashahkaku were selected for analysis. The parameters, like Temperature, pH, conductivity, hardness, alkalinity, total dissolved solids, chemical oxygen demands, phosphate, nitrate, nitrite, major cations (Na, K, Ca, Mg) and heavy/trace metals, were studied. The results were compared with National environmental Quality standards (NEQS). It was further observed that when effluents of industries join fresh water of stream, lakes or rivers, this causes severe water-pollution and damages the flora and fauna. Suggestions for effective control of water-pollution are also given. (author)

  3. Influence of the meteorological parameters on CFCs and SF6 concentration in the air of Krakow, Poland

    Science.gov (United States)

    Bielewski, Jarosław; Najman, Joanna; Śliwka, Ireneusz; Bartyzel, Jakub; Rosiek, Janusz

    2013-04-01

    key words: gas chromatography, trace gases, CFCs and SF6 measurements in urban area. Halogenated compounds (chlorofluorocarbons-CFCs), both natural and industrial, so-called freons, currently exist as trace gases in the entire human environment. The CFCs cause ozone depletion in the stratosphere. Moreover CFCs and SF6 take part in intensification of the greenhouse effect. The decisions of the Vienna Convention (1985) and of the Montreal Protocol (1987) limited the world production level of CFCs in the year 1989 at least 35% after 2004, 90% after 2015 and total reduction after year 2030. On account of international agreements, the measurements of CFCs and SF6 in air were started. Measurement "clean" stations were situated at places outside of urban areas influence and gathered on world program - AGAGE (Advanced Global Atmospheric Gases Experiment). One of these stations is Mace Head (Ireland, 53o N, 10o W), which participates in AGAGE since 1987 [1] and in European InGOS (Integrated non-CO2 Greenhouse gas Observing System) program since 2011. Similar research is also conducted in Central Europe, in urban area of Krakow (Poland, 50o N, 19o E) since 1997. The work discusses results from 15 years of concentration measurements (in the years 1997-2012) of selected halocarbons and SF6 in Krakow. To obtain concentrations of measured compounds the mathematical procedure has been used, where concentrations were calculated using a five points Lagrange's interpolation method. Using temporary measurement data were determined daily arithmetic means and their standard deviations. Based on these data, efficiency of Montreal Protocol legislation, implemented in Poland (The Journal of Laws No. 52) could be assessed [2]. Additionally cut-off filtration method was used to estimate trend of the base line of individual air pollutant. Rejected exceedances of base lines were corelated with meteorological characteristics of Krakow region to evaluate possible sources of pollution. The

  4. 肉鸡高、低脂系体脂性状和血清生化指标的比较分析%Comparative analysis of body fat traits and serum biochemical parameters between broiler lines divergently selected for abdominal fat content

    Institute of Scientific and Technical Information of China (English)

    王海威; 王启贵; 王守志; 户国; 李辉

    2009-01-01

    以东北农业大学肉鸡高、低腹脂双向选择品系为试验材料,分析第11、12世代肉鸡体重、体脂性状和血清生化指标在两系间的差异.结果表明,在体重和体脂性状方面,肉鸡高脂系的腹脂重、腹脂率、肝脏重极显著高于低脂系肉鸡的相应性状(P0.05).在血清生化指标方面,高脂系肉鸡血清中HDL-C、VLDL、FFA、apoB和胰岛素(Insulin)水平高于低脂系肉鸡的相应指标(P0.05).上述结果表明,对肉鸡腹脂率的持续定向选择对血液中脂质代谢各项生化指标具有显著的影响.有助于理解肉鸡血脂代谢与体脂沉积的关系,对低脂肉鸡的进一步选育具有积极的指导意义.%The fat and lean line broilers in the 11th and 12th generation were studied, which were divergent selection by abdominal fat percentage and plasma VLDL concentration. The difference of body weight, fat traits and serum biochemical parameters were analyzed in the two lines. The results showed that the difference of body weight at 7 weeks of age was not significant in two lines, and the difference of abdominal fat weight, abdominal fat percentage, liver weight, and liver fat percentage was very signifi-cant in two lines, and lean line were lower(P<0.01, P<0.05). However, the pectoralis intramuscular fat percentage, and leg intramuscular fat percentage in lean line were higher than those of fat line (P<0.01). The difference of TG and TC concentration in serum was not significant in two lines, and the difference of HDL-C, VLDL, FFA, Insulin and apoB concentration in serum was very significant in two lines, and lean line were lower (P<0.01). However, the Glu, and LDL-C concentration in serum in lean line were higher than those of fat line (P<0.01). So the current study could draw a conclusion that abdominal fat percentage of broilers continued directional selection have a significant impact on the blood biochemical parameters of lipid metabolism.

  5. Tissue Residues, Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken

    Directory of Open Access Journals (Sweden)

    Mossad Elsayed

    2014-01-01

    Full Text Available The aim of this study was to determine the blood and tissue concentrations profile and effect of tilmicosin on some hematological and biochemical parameters in broiler chicken. Fifty clinically healthy Hubbard chickens were orally administered 25 mg/kg BW of tilmicosin once daily for 5 consecutive days. Tissue residues of tilmicosin in slaughtered healthy chicken could not be detected by microbiological assay in all tested tissues except in lung (at 96 hours and liver and kidneys (at 72 hours after last administration. Tilmicosin caused temporary decrease in the RBCs and WBCs counts and has no effect on hemoglobin (Hb and packed cell volume concentration (PCV. Also, the effect of tilmicosin on some biochemical parameters was as follows: the concentrations of creatinine, uric acid, electrolytes (sodium, potassium, and calcium, glucose, AST, ALT, ALP, and HDL-cholesterol in the serum of treated chicken did not change in response to the repeated oral administration of tilmicosin. There were only a temporary significant decrease in total protein and albumin concentrations and a significant increase in cholesterol and triglycerides concentrations. Chicken must not be slaughtered before 4 days from the stopping of tilmicosin administration. Tilmicosin makes temporary changes on hematological and biochemical parameters in broiler chicken.

  6. Tissue Residues, Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken

    Science.gov (United States)

    Elsayed, Mossad; Elkomy, Ashraf; Morad, Mohamed

    2014-01-01

    The aim of this study was to determine the blood and tissue concentrations profile and effect of tilmicosin on some hematological and biochemical parameters in broiler chicken. Fifty clinically healthy Hubbard chickens were orally administered 25 mg/kg BW of tilmicosin once daily for 5 consecutive days. Tissue residues of tilmicosin in slaughtered healthy chicken could not be detected by microbiological assay in all tested tissues except in lung (at 96 hours) and liver and kidneys (at 72 hours) after last administration. Tilmicosin caused temporary decrease in the RBCs and WBCs counts and has no effect on hemoglobin (Hb) and packed cell volume concentration (PCV). Also, the effect of tilmicosin on some biochemical parameters was as follows: the concentrations of creatinine, uric acid, electrolytes (sodium, potassium, and calcium), glucose, AST, ALT, ALP, and HDL-cholesterol in the serum of treated chicken did not change in response to the repeated oral administration of tilmicosin. There were only a temporary significant decrease in total protein and albumin concentrations and a significant increase in cholesterol and triglycerides concentrations. Chicken must not be slaughtered before 4 days from the stopping of tilmicosin administration. Tilmicosin makes temporary changes on hematological and biochemical parameters in broiler chicken. PMID:24808972

  7. Bioimpedance spectroscopy as technique of hematological and biochemical analysis of blood

    Science.gov (United States)

    Malahov, M. V.; Smirnov, A. V.; Nikolaev, D. V.; Melnikov, A. A.; Vikulov, A. D.

    2010-04-01

    Bioimpedance spectroscopy may become a useful method for the express analysis and monitoring of blood parameters. The aim of this study was to identify biochemical and hematological parameters of blood that can be accurately predicted by means of bioimpedance technique. Hematological (red blood cell and white blood cell parameters) and biochemical (total proteins, albumins, fibrinogen, sodium, potassium, chloride ion concentrations in plasma) parameters were measured with a hematological analyzer and routine methods. Bioimpedance spectroscopy of the whole blood (1.5 ml) in frequency range 5-500 kHz (31 frequencies) was performed using BIA analyzer ABC-01 "Medass". Frequency relationships of resistance and reactance of the whole blood and the parameters of the Cole model were investigated. Close simple and multiple correlations of bioimpedance indices were observed only with erythrocyte parameters (Ht, Hb, RBC). Thus bioimpedance analysis of the whole blood can accurately predict red cell parameters but it is less effective for estimation of plasma biochemical and white cell parameters.

  8. Molecular and biochemical studies on bovine ephemeral fever

    Directory of Open Access Journals (Sweden)

    Nahed S. Thabet; Emad W. Ghazy; Mohamed A. Nayel; Mohamed Abo-Elkhair

    2011-05-01

    Full Text Available Bovine ephemeral fever (BEF in cattle has been reported to be associated with a range of biochemical changes which are similar to those seen in milk fever. This study aimed to clarify the biochemical alterations that associate infection of cattle with BEF with special references to the mechanisms involved in the development of hypocalcemia. The study was conducted on 30 cases of cattle infected with BEF based on the characteristic clinical signs which were confirmed by isolation of virus and RT-PCR. Another 6 healthy cows were used in the study as control. The evaluated parameters included biochemical variables such as serum values of total protein (TP, albumin (Alb, glucose (Glu, total calcium (tCa, ionized calcium (iCa, inorganic phosphorus (P, magnesium (Mg, sodium (Na, potassium (K, chloride (Cl, creatinine (Cr, blood urea nitrogen (BUN and serum activity of alkaline phosphatase (ALP. Hormonal profile included parathyroid hormone (PTH, insulin (Ins, and cortisol (Cor. The results showed that BEF-infected animals demonstrated a significant decrease (P<0.05 in serum concentrations of TP, Glo, iCa, P, Na, K, BUN and ALP while the mean values of serum levels of Glu and Cl were significantly increased (P<0.05. The mean values of serum levels of PTH were significantly decreased (P<0.05 while serum concentrations of Ins and Cor showed a significant increase. It was concluded that the clinical signs of bovine ephemeral fever are related to the hypocalcemia resulting from suppression of parathyroid hormone which seems to be mediated by respiratory alkalosis caused by the disease. This explanation needs future studies to provide a direct link between measurement of blood indicators of acid-base status, blood biochemical parameters and urine analysis. However, this work can provide a good knowledge about the pathogenesis of the disease that can lead to better management and proper treatment.

  9. Assessment and influence of operational parameters on the TiO2 photocatalytic degradation of sodium benzene sulfonate under highly concentrated solar light illumination

    International Nuclear Information System (INIS)

    Sodium benzene sulfonate (BS) was decomposed in aqueous TiO2 dispersions under highly concentrated solar light illumination to examine the photocatalytic characteristics of a parabolic round concentrator (PRC) reactor to degrade the pollutant without visible light absorption. The effects of such operational parameters as initial concentration, volume of the aqueous BS solution, oxygen purging, and TiO2 loading on the kinetics of decomposition of BS were investigated. An effective photodegradation necessitates a suitable combination of initial volume and concentration of BS solution. Relative to atmospheric air, oxygen purging significantly accelerates the degradation process at high initial concentrations of BS (0.40 mM or 1.0 mM). Optimal TiO2 loading was 9 gl -1, greater than previously reported. Elimination of TOC (total organic carbon) followed pseudo first-order kinetics in the initial stages of the photodegradation process. The relative photonic efficiency for the photodegradation of BS is ζrel=1.0. (Author)

  10. The hydrogen concentration as parameter to identify natural attenuation processes of volatile chlorinated hydrocarbons in ground water; Die Wasserstoffkonzentration als Parameter zur Identifizierung des natuerlichen Abbaus von leichtfluechtigen Chlorkohlenwasserstoffen (LCKW) im Grundwasser

    Energy Technology Data Exchange (ETDEWEB)

    Alter, M.D.

    2006-06-15

    In this study, the hydrogen concentration as parameter to identify natural attenuation processes of volatile chlorinated hydrocarbons was investigated. The currently accepted and recommended bubble strip method for hydrogen sampling was optimized, and a storage method for hydrogen samples was developed. Furthermore batch experiments with a dechlorinating mixed culture and pure cultures were carried out to study H{sub 2}-concentrations of competing redox processes. The extraction of hydrogen from ground water was optimized by a reduced inlet diameter of the usually applied gas sampling bulbs, allowing a maximal turbulent ow and gas transfer. With a gas volume of 10 ml and flow rates of 50 to 140 ml/min, the course of extraction almost followed the theoretical course of equilibration. At flow rates > 100 ml/min a equilibrium of 98% was achieved within 20 min. Until recently it was generally accepted that hydrogen samples can be stored only for 2 hours and therefore have to be analyzed immediately in the eld. Here, it was shown that eld samples can be stored for 1-3 days until analysis. For the dechlorination of tetrachloroethene (PCE), a hydrogen threshold concentration of 1-2 nM was found with the dechlorinating mixed culture as well as with a pure culture of Sulfurospirillum multivorans in combination with another pure culture Methanosarcina mazei. No dechlorination was detectable below this concentration. With the dechlorinating mixed culture, this finding is valid for all successive dechlorination steps until ethene. The hydrogen threshold concentration for denitrification were below the detection limit of 0,2 nM with the dechlorinating mixed culture. A threshold concentration of 3,1-3,5 nM was found for sulphate reduction and a threshold of 7-9 nM H{sub 2} for hydrogenotrophic methanogenesis. This implies that the natural dechlorination at contaminated sites is preferred to competing processes like sulphate reduction and methanogenesis. The threshold

  11. Effects of heavy metal (Pb) concentration on some growth parameters of plants grown in lead polluted soil under organic fertilizer amendment

    Institute of Scientific and Technical Information of China (English)

    Ojo M Oseni; Adekunle A Adelusi; Esther O Dada; Abdulfatai B Rufai

    2016-01-01

    This study investigated morphological variation and biomass accumulation that occurred inSida acuta andChromolaena odorata plants grown in lead polluted soil under organic fertilizer amendment. The study was carried out in the screen house at the Biological Gardens of the Obafemi Awolowo University, Ile-Ife, Osun State. The experiment was a factorial combination of one heavy metal (Pb) at five levels of concentration (0, 200, 400, 800 and 1,000 mg/kg) in a completely randomized design, and were replicated three times for each of the two plants and two levels (0 g/kg and 9.4 g/kg) of organic fertilizer (OBD-Plus). Each pot was filled with 5 kg of air-dried and sieved soil and placed on a plastic tray for the collection of excess water. Two weeks after planting, seedlings of uniform height were transplanted from the nursery to experimental pots at the rate of one seedling per pot and grown for 10 weeks. The growth parameters of the plants were biomonitored for 7 weeks. After 10 weeks of treatment, the plants were harvested and dried to calculate the biomass accumulation. The two plant species performed better under fertilizer application than without it. For each of the plant species the growth parameters decreased as the levels of Pb concentration increased. Furthermore, the plants' biomass decreased significantly (p<0.05) as the levels of Pb concentration increased. The organic fertilizer helped to improve the plants' performance in lead-polluted soil.

  12. Effects of conjugated linoleic acids and dietary concentrate proportion on performance, milk composition, milk yield and metabolic parameters of periparturient dairy cows.

    Science.gov (United States)

    Petzold, Maria; Meyer, Ulrich; Kersten, Susanne; Spilke, Joachim; Kramer, Ronny; Jahreis, Gerhard; Dänicke, Sven

    2013-06-01

    The study aimed to examine effects of supplemented conjugated linoleic acids (CLA) to periparturient cows receiving different concentrate proportions ante partum (a.p.) to investigate CLA effects on lipid mobilisation and metabolism. Compared to adapted feeding, a high-concentrate diet a.p. should induce a ketogenic metabolic situation post partum (p.p.) to better understand how CLA works. Sixty-four pregnant German Holstein cows had ad libitum access to partial mixed rations 3 weeks prior to calving until day 60 p.p. Ante partum, cows received control fat (CON) or a CLA supplement at 100 g/d, either in a low-concentrate (CON-20, CLA-20) or high-concentrate diet (CON-60, CLA-60). Post partum, concentrate proportion was adjusted, while fat supplementation continued. After day 32 p.p., half of the animals of CLA-groups changed to CON supplementation (CLA-20-CON, CLA-60-CON). A ketogenic metabolic situation p.p. was not achieved and therefore impacts of CLA could not be examined. Live weight, milk yield and composition, blood parameters remained unaffected by the treatments. Only a slightly reduced milk fat yield (not significant) was recorded for Group CLA-20. The proportion of trans-10,cis-12 (t10,c12) CLA in milk fat was significantly increased in CLA-groups compared to CON-groups. With the exception of a reversible CLA effect on milk fat in Group CLA-20, no post-treatment effects occurred. Dry matter intake (DMI) of Group CLA-60 was highest before calving, resulting in a significantly improved estimated energy balance after calving. Ante partum, net energy intakes were significantly increased in high-concentrate groups. Overall, supplemented CLA preparation did not relieve metabolism and lipid mobilisation of early lactating cows. But feeding CLA in a high-concentrate diet a.p. seems to increase DMI and thereby improve the energy balance of cows immediately after calving.

  13. Vitamin A Affects Growth Performance and Serum Biochemical Parameters of Geese%维生素A对鹅生长性能及血清生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    孙淑洁; 王宝维; 葛文华; 张名爱; 李文立

    2012-01-01

    This experiment was conducted to study the effects of vitamin A on growth performance, serum biochemical parameters and tissue vitamin A content of Qingnonghui geese. Two hundred Qingnonghui geese at 1 day of age were selected and randomly divided into 5 groups with 4 replicates in each group and 10 geese in each replicate. Five groups were supplemented with 0, 1 500, 3 000, 6 000 and 12 000 IU/kg vitamin A in the basal diets, respectively. The experiment lasted for twelve weeks. The results showed as follows; 1) supplemental vitamin A significantly increased daily weight gain and daily feed intake (P 0. 05). 2) At 4 weeks of age, supplemented 6 000 IU/kg vitamin A significantly decreased serum uric acid content (P 0. 05) ; at 12 weeks of age, supplemented 6 000 IU/kg vitamin A significantly decreased the contents of serum blood glucose and uric acid (P 0. 05). 3) The serum and liver vitamin A content increased significantly with the increase of dietary vitamin A supplemental levels (P <0. 01). In conclusion, consideration from the growth performance, the optimal dietary levels of vitamin A are 8 000 IU/kg at weeks 1 to 4, and 9 000 IU/kg at weeks 5 to 12. While consideration from the serum biochemical parameters, the optimal dietary level of vitamin A is 6 000 IU/kg at weeks 1 to 4 and 5 to 12.%本试验旨在研究不同水平维生素A对青农灰鹅生长性能、血清生化指标及组织中维生素A含量的影响.选用1日龄青农灰鹅200只,随机分为5组,每组4个重复,每个重复10只鹅.各组在玉米-豆粕型基础饲粮中分别添加0、1 500、3 000、6 000和12 000 IU/kg的维生素A.试验期12周.结果表明:1)饲粮添加维生素A能显著提高1~4、5~12周龄青农灰鹅平均日增重和平均日采食量(P<0.05),降低死淘率,但对料重比影响不显著(P>0.05).2)4周龄时,添加6 000 IU/kg维生素A显著降低了血清尿酸含量(P<0.05),添加3 000和6 000 IU/kg维生素A极显著提高了

  14. Estimating the evolution of atrazine concentration in a fractured sandstone aquifer using lumped-parameter models and taking land-use into account

    Science.gov (United States)

    Farlin, J.; Gallé, T.; Bayerle, M.; Pittois, D.; Braun, C.; El Khabbaz, H.; Maloszewski, P.; Elsner, M.

    2012-04-01

    The European water framework directive and the groundwater directive require member states to identify water bodies at risk and assess the significance of increasing trend in pollutant concentration. For groundwater bodies, estimating the time to trend reversal or the pollution potential of the different sources present in the catchment require a sound understanding of the hydraulic behaviour of the aquifer. Although numerical groundwater models can theoretically be used for such forecasts, their calibration remains in many real-world cases problematic. A more parsimonious lumped-parameter model was applied to predict the evolution of atrazine concentration in springs draining a fractured sandstone aquifer in Luxembourg. Despite a nationwide ban in 2005, spring water concentrations of both atrazine and its metabolite desethylatrazine still had not begun to decrease four years later. The transfer function of the model was calibrated using tritium measurements and modified to take into account the fact that whereas tritium is applied uniformly over the entire catchment, atrazine was only used in areas where cereals are grown. We could also show that sorption processes in the aquifer can be neglected and that including pesticide degradation does not modify the shape of the atrazine breakthrough, but only affects the magnitude of the predicted spring water concentration. Results indicate that due to the large hydraulic inertia of the aquifer, trend reversal should not be expected before 2018.

  15. In vivo and in vitro changes in neurochemical parameters related to mercury concentrations from specific brain regions of polar bears (Ursus maritimus).

    Science.gov (United States)

    Krey, Anke; Kwan, Michael; Chan, Hing Man

    2014-11-01

    Mercury (Hg) has been detected in polar bear brain tissue, but its biological effects are not well known. Relationships between Hg concentrations and neurochemical enzyme activities and receptor binding were assessed in the cerebellum, frontal lobes, and occipital lobes of 24 polar bears collected from Nunavik (Northern Quebec), Canada. The concentration-response relationship was further studied with in vitro experiments using pooled brain homogenate of 12 randomly chosen bears. In environmentally exposed brain samples, there was no correlative relationship between Hg concentration and cholinesterase (ChE) activity or muscarinic acetylcholine receptor (mAChR) binding in any of the 3 brain regions. Monoamine oxidase (MAO) activity in the occipital lobe showed a negative correlative relationship with total Hg concentration. In vitro experiments, however, demonstrated that Hg (mercuric chloride and methylmercury chloride) can inhibit ChE and MAO activities and muscarinic mAChR binding. These results show that Hg can alter neurobiochemical parameters but the current environmental Hg exposure level does have an effect on the neurochemistry of polar bears from northern Canada. PMID:25264143

  16. Investigating how fundamental parameters of XRF sample preparation and analysis affect the observed elemental concentration: an experiment using fluvial sediment from Sabah, Borneo.

    Science.gov (United States)

    Higton, Sam; Walsh, Rory

    2015-04-01

    X-Ray Fluorescence (XRF) is an important technique for measuring the concentrations of geochemical elements and inorganic contaminants adsorbed to sediments as an input to sediment tracing methods used to evaluate sediment transport dynamics in river catchments. In addition to traditional laboratory-based XRF instruments, the advent of increasingly advanced portable handheld XRF devices now mean that samples of fluvial sediment can be analysed in the field or in the laboratory following appropriate sample preparation procedures. There are limitations and sources of error associated with XRF sample preparation and analysis, however. It is therefore important to understand how fundamental parameters involved in sample preparation and analysis, such as sample compression and measurement exposure duration, affect observed variability in measurement results. Such considerations become important if the resulting measurement variability is high relative to the natural variability in element concentrations at a sample site. This paper deployed a simple experimental design to assess the impacts of varying a number of sample preparation and XRF analysis parameters on recorded measurements of elemental concentrations of the fine fraction (XRF elemental analyser. Helium purging was used on both machines to enable measurement of lighter geochemical elements. Sediment sub-samples were taken from a larger homogenised sample from a sediment core taken from an in-channel lateral bench deposit of the Brantian river in Sabah, Borneo; the core site is being used for research into multi-proxy sediment fingerprinting as part of the Stability of Altered Forest Ecosystems (SAFE) project. Some fundamental sample preparation procedures consistent with US EPA Method 6200 were applied to all sediment samples in order to explore key variables of interest. All sediment samples were air-dried to constant weight and sample quantity was sufficient to satisfy the assumption of 'infinite thickness

  17. 复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响%Milk production,nitrogen excretion and blood biochemical parameter responses to di-etary addition of compound yeast cultures in dairy cows

    Institute of Scientific and Technical Information of China (English)

    王玲; 吕永艳; 程志伟; 杜高唐; 李金林; 付石军; 孙国强

    2015-01-01

    为研究饲料中添加复合酵母培养物对奶牛产奶性能、氮排放及血液生化指标的影响,选取年龄、体重、产奶量及泌乳期相近(135±15)d 的荷斯坦奶牛24头,随机分为4组,每个处理6个重复,对照组和3个试验组的复合酵母培养物添加量分别为精料浓度的0,0.8%,1.0%,1.2%,随精料饲喂,测定产奶量、乳成分、氮排放及血液生化指标,结果表明,1)试验2组日均产奶量显著高于对照组(P <0.05),各试验组分别比对照组提高8.48%,10.05%,8.97%。2)复合酵母培养物能显著提高乳脂和乳蛋白率(P <0.05),显著降低牛奶体细胞数(P <0.05),以试验2组最低。3)在氮排放量上,试验2组显著低于对照组(P <0.05),各试验组比对照组分别降低8.47%,12.01%,9.36%。4)在血液生化指标方面,复合酵母培养物能提高血清中总蛋白、球蛋白、血糖、胰岛素水平(P <0.05),降低尿素氮水平(P <0.05)。由此可见,本试验条件下,综合考虑产奶量、乳成分、氮排放及血液生化指标,复合酵母培养物的最佳添加量为精料浓度的1.0%。%This experiment was conducted to determine the effects of compound yeast cultures on the milk pro-duction,nitrogen excretion and blood biochemical parameters of dairy cows.A total of 24 Holstein lactating cows with similar parity,weight,milk yield and lactation stage (135±15 day in milk)were equally assigned to four groups,including one control group and 3 treatment groups,with dietary supplementation of compound yeast cultures at concentrations of 0,0.8%,1.0% and 1.2%,respectively.Milk yield,milk composition,ni-trogen excretion and blood biochemical parameters of each cow were recorded.The results showed:1 )The cows in the respective treatment groups had 8.48%,10.05%,8.97% higher daily milk yield than cows in the control

  18. [Effectiveness of specific immunotherapy in the treatment of children and youngsters suffering from atopic dermatitis. Part III. Serum concentrations of selected immunologic parameters].

    Science.gov (United States)

    Silny, Wojciech; Czarnecka-Operacz, Magdalena; Silny, Pawel

    2005-01-01

    Etiology and pathomechanism of atopic dermatitis still remains partially unclear and therefore contemporary methods of treatment are not always satisfactory from the clinical standpoint. The aim of this study was to evaluate selected immunological parameters (tIgE, ECP, sIL-2R, IFN-gamma, IL-4,IL-5) in sera of atopic dermatitis patients in the course of specific immunotherapy performed for the time period of 3 years. Novo-Helisen Depot allergy vaccines of appropriate composition were used for the treatment of 36 children and youngsters with atopic dermatitis, allergic to house dust mites (24 patients) and grass pollen allergens (12 patients). The control group consisted of 20 patients with atopic dermatitis and analogous IgE-mediated airborne allergy who were treated with conventional methods. There was a clear difference between two investigated groups of patients in terms of immunological parameters. In the group treated with allergy vaccines serum concentrations of total IgE and ECP tended to decrease (p < 0.001) as well as sIL-2R (p < 0.01). On the contrary in the control group serum tIgE increased and IL-4 as well as IL-5 concentrations tended to increase significantly (p < 0.01; p < 0.05 respectively).

  19. Effect of whey protein concentrate and sodium chloride addition plus tumbling procedures on technological parameters, physical properties and visual appearance of sous vide cooked beef.

    Science.gov (United States)

    Szerman, N; Gonzalez, C B; Sancho, A M; Grigioni, G; Carduza, F; Vaudagna, S R

    2007-07-01

    Beef muscles cooked by the sous vide system were evaluated for the effects of pre-injection tumbling, brine addition and post-injection tumbling on technological parameters, physical properties, visual appearance and tissue microstructure. The muscles were injected at 120% (over original weight) with a brine formulated to give a concentration of 3.5% whey protein concentrate and 0.7% sodium chloride on an injected raw product basis. Pre-injection tumbling did not affect most of the evaluated parameters. Brine addition reduced significantly the cooking and total weight losses. Total weight loss was 7.2% for injected muscles, and significantly higher (28.2%) for non-injected ones. Brine incorporation increased pH and reduced shear force values of cooked muscles. Extended post-injection tumbling (5rpm-10h) improved brine distribution and visual appearance, and also diminished the shear force values of cooked muscles. However, this treatment increased the weight losses of post-injection tumbling and cooking-pasteurization stages.

  20. Effect of whey protein concentrate and sodium chloride addition plus tumbling procedures on technological parameters, physical properties and visual appearance of sous vide cooked beef.

    Science.gov (United States)

    Szerman, N; Gonzalez, C B; Sancho, A M; Grigioni, G; Carduza, F; Vaudagna, S R

    2007-07-01

    Beef muscles cooked by the sous vide system were evaluated for the effects of pre-injection tumbling, brine addition and post-injection tumbling on technological parameters, physical properties, visual appearance and tissue microstructure. The muscles were injected at 120% (over original weight) with a brine formulated to give a concentration of 3.5% whey protein concentrate and 0.7% sodium chloride on an injected raw product basis. Pre-injection tumbling did not affect most of the evaluated parameters. Brine addition reduced significantly the cooking and total weight losses. Total weight loss was 7.2% for injected muscles, and significantly higher (28.2%) for non-injected ones. Brine incorporation increased pH and reduced shear force values of cooked muscles. Extended post-injection tumbling (5rpm-10h) improved brine distribution and visual appearance, and also diminished the shear force values of cooked muscles. However, this treatment increased the weight losses of post-injection tumbling and cooking-pasteurization stages. PMID:22060988

  1. Reply to Effect of concentration of organic matter on optical maturity parameters. Interlaboratory results of the organic matter concentration working group of the ICCP. Discussion by Vinay K. Sahay

    Science.gov (United States)

    Mendonca, Filho J.G.; Araujo, C.V.; Borrego, A.G.; Cook, A.; Flores, D.; Hackley, P.; Hower, J.C.; Kern, M.L.; Kommeren, K.; Kus, J.; Mastalerz, Maria; Mendonca, J.O.; Menezes, T.R.; Newman, J.; Ranasinghe, P.; Souza, I.V.A.F.; Suarez-Ruiz, I.; Ujiie, Y.

    2011-01-01

    This reply is motivated by Sahay's comments on the paper published by Mendon??a Filho et al. (2010) dealing with the effect of concentration of an organic matter on optical maturity parameters. Four points were raised by Sahay: suggestion to use of chemical parameters to assess the effect of isolation, indication that suppression of vitrinite reflectance in liptinite-rich rocks was insufficiently addressed, discussion on the way to deal with the existence of multiple vitrinite populations in a dispersed organic matter, and contradictory explanation of results involving the influence of isolation procedure on fluorescence properties but no effect on vitrinite reflectance. The four points were separately addressed being the two first ones out of the scope of the paper. The existence of multiple vitrinite populations is a well-recognized problem whose importance in the results could be addressed because the participants provided individual records of vitrinite reflectance. These results indicated that election of different populations was not a major problem in the results. The influence of isolation procedure on the fluorescence spectra of alginite while the vitrinite reflectance remains unaltered is not considered contradictory because both parameters are measured on different components which may have a different response to the acid treatment. ?? 2011 Elsevier B.V.

  2. Effect of a mixture of micronutrients, but not of bovine colostrum concentrate, on immune function parameters in healthy volunteers: a randomized placebo-controlled study

    Science.gov (United States)

    Wolvers, Danielle AW; van Herpen-Broekmans, Wendy MR; Logman, Margot HGM; van der Wielen, Reggy PJ; Albers, Ruud

    2006-01-01

    Background Supplementation of nutritional deficiencies helps to improve immune function and resistance to infections in malnourished subjects. However, the suggested benefits of dietary supplementation for immune function in healthy well nourished subjects is less clear. Among the food constituents frequently associated with beneficial effects on immune function are micronutrients such as vitamin C, vitamin E, β-carotene and zinc, and colostrum. This study was designed to investigate the effects these ingredients on immune function markers in healthy volunteers. Methods In a double-blind, randomized, parallel, 2*2, placebo-controlled intervention study one hundred thirty-eight healthy volunteers aged 40–80 y (average 57 ± 10 y) received one of the following treatments: (1) bovine colostrum concentrate 1.2 g/d (equivalent to ~500 mg/d immunoglobulins), (2) micronutrient mix of 288 mg vitamin E, 375 mg vitamin C, 12 mg β-carotene and 15 mg zinc/day, (3) combination of colostrum and micronutrient mix, or (4) placebo. Several immune function parameters were assessed after 6 and 10 weeks. Data were analyzed by analysis of variance. Groups were combined to test micronutrient treatment versus no micronutrient treatment, and colostrum treatment versus no colostrum treatment. Results Overall, consumption of the micronutrient mix significantly enhanced delayed-type hypersensitivity (DTH) responses (p < 0.05). Adjusted covariance analysis showed a positive association between DTH and age. Separate analysis of younger and older age groups indicated that it was the older population that benefited from micronutrient consumption. The other immune function parameters including responses to systemic tetanus and oral typhoid vaccination, phagocytosis, oxidative burst, lymphocyte proliferation and lymphocyte subset distribution were neither affected by the consumption of micronutrients nor by the consumption of bovine colostrum concentrate. Conclusion Consumption of bovine

  3. Changes in Selected Biochemical Indices Resulting from Various Pre-sampling Handling Techniques in Broilers

    OpenAIRE

    Chloupek Petr; Bedanova Iveta; Chloupek Jan; Vecerek Vladimir

    2011-01-01

    Abstract Background Since it is not yet clear whether it is possible to satisfactorily avoid sampling-induced stress interference in poultry, more studies on the pattern of physiological response and detailed quantification of stress connected with the first few minutes of capture and pre-sampling handling in poultry are required. This study focused on detection of changes in the corticosterone level and concentrations of other selected biochemical parameters in broilers handled in two differ...

  4. Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758): Biochemical response

    OpenAIRE

    Mahdi Banaee; Behzad Nematdoust Haghi; Ahmed Th. A. Ibrahim

    2014-01-01

    Chlorpyrifos, an organophosphate pesticide, is widely used to control pests in agriculture farms and orchards of fruit trees. In this study, the fish were exposed to sub-lethal concentrations of chlorpyrifos which were determined based on numerical value of 96 h LC50. Blood was sampled after 10, 20 and 30 days and biochemical parameters including glucose, total protein, albumin, globulin, triglyceride and cholesterol levels, and aspartate aminotransferase (AST), alanine aminotransferase (ALT)...

  5. [The biochemical and rheological properties of the blood during the performance of angiography using Ultravist].

    Science.gov (United States)

    Poliaev, Iu A; Lazarev, V V; Kulikova, I S; Shimanovskiĭ, N L; Usenko, A N; Smirnova, O Iu; Isakov, Iu F; Sergeev, P V

    1996-01-01

    It is shown that Ultravist makes it possible to receive clear visualization of vessel channel in the zone "of interest" without changing functional conditions of heart vascular system and biochemical blood parameters (level of erythrocytes, bilirubine, urine nitrogen, activity of aspartataminotransferase). In concentration 30 mg/ml in vitro and in vivo Ultravist decreases a viscosity limit not affecting other rheological properties of blood. A mechanism of the found Ultravist effect and prospects of its application in practice for children are considered.

  6. Tissue Residues, Hematological and Biochemical Effects of Tilmicosin in Broiler Chicken

    OpenAIRE

    Mossad Elsayed; Ashraf Elkomy; Mohamed Aboubakr; Mohamed Morad

    2014-01-01

    The aim of this study was to determine the blood and tissue concentrations profile and effect of tilmicosin on some hematological and biochemical parameters in broiler chicken. Fifty clinically healthy Hubbard chickens were orally administered 25 mg/kg BW of tilmicosin once daily for 5 consecutive days. Tissue residues of tilmicosin in slaughtered healthy chicken could not be detected by microbiological assay in all tested tissues except in lung (at 96 hours) and liver and kidneys (at 72 hour...

  7. Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes

    Directory of Open Access Journals (Sweden)

    Sandhya S. Chaudhary

    2015-06-01

    Full Text Available Aim: This study was conducted to evaluate the impact of hot dry, hot humid and comfortable season on physiological, hematological, biochemical, and oxidative stress parameters in Surti buffaloes. Materials and Methods: Ten lactating Surti buffaloes of similar physiological status were selected. Based on the temperature-humidity index (THI, their natural exposure to the environment was categorized as hot dry (THI1, hot humid (THI2 and moderate winter/comfort season (THI3. Blood/serum samples were collected and analyzed for physiological, hematological, biochemical, and oxidative stress parameters. The results were analyzed using standard statistical methods. Results: With increase in THI, significant rise in physiological parameters such as respiration rate (RR, hematological parameters such as red blood cell (RBC, hematocrit, hemoglobin (Hb and mean cell Hb concentration (MCHC, biochemical parameters such as alanine aminotransferase (ALT, Na, K, creatinine, blood urea nitrogen, Mn, Cu and Zn, hormones such as cortisol and oxidative stress parameters such as glutathione peroxidase (GPx, superoxide dismutase (SOD, lipid peroxide (LPO and total antioxidant status (TAS and significant decline in glucose, cholesterol and triiodothyronine (T3 was observed. Conclusion: It was concluded that THI is a sensitive indicator of heat stress and is impacted by ambient temperature more than the relative humidity in buffaloes. Higher THI is associated with significantly increased RR, total RBC count, Hb, hematocrit, MCHC, ALT, urea, sodium, creatinine, triiodothyronine, SOD, GPx, LPO and TAS and with significant decrease in glucose, cholesterol and triiodothyronine (T3.

  8. [Effect of the increasing levels of soil radioactive pollution on the biochemical composition of plants].

    Science.gov (United States)

    Gromova, V S; Pchelenok, O A; Kozlova, N M

    2012-01-01

    The study was undertaken to study a relationship between the changes of some parameters of the biochemical and mineral composition of different plants, such as rape, pods, and lentil, and the levels of soil radiation pollution, by using the conventional methods. Radioactive pollution of dark-grey forest soils was found to cause a change in the biochemical composition of plant seeds even at the level of cesium 137 (137Cs) within the present temporary permissible levels (TPL) (600 Bq/kg): there were elevated concentrations of salts of potassium, phosphorus, ammonia nitrogen, catechols, sucrose, and some amino acids. With the radioactive cesium level exceeding the TPL, biochemical changes in the seeds depended on the species of the plants: in the rape seeds, the additional formation of sucrose and amino acids continued, but less intensively than with its lower radiation; the pod beans were significantly positively correlated with the increasing amounts of catechols.

  9. Biochemical studies of M. incognita and M. javanica towards their identification.

    Science.gov (United States)

    Sharma, Charul; Singh, Sohini

    2016-06-01

    Biochemical analysis is a reliable, quick, yet effective way for profiling any organism. The same tools when employed in taxonomic queries, they aid substantially and support the process. Taxonomic queries have been since beginning presenting numerable challenges towards the studies of different plant parasitic nematodes. In the present study various biochemical tests have been employed to assess the differentiation among the two dominant sps. of Meloidogyne i.e., M. incognita and M. javanica. Some of the biochemical parameters considered were the specific concentrations of carbohydrates and cholesterol and were monitored in the juvenile and the female of the respective species. Results have been discussed in details along with its employability towards the taxonomical studies especially with reference to plant parasitic nematodes. PMID:27413284

  10. 不同属种肉类对大鼠血清生化指标与抗氧化能力的影响%Effects of different species meats on serum biochemical parameters and antioxidant status in rats

    Institute of Scientific and Technical Information of China (English)

    邓宇; 高峰; 周光宏

    2011-01-01

    探讨了不同属种肉类对大鼠血清生化指标和抗氧化机能的影响.试验选取体重约177 g的雄性SD大鼠90只,随机分为3组即狗肉组、羊肉组、鸭肉组,分别将日粮中的主要蛋白源替换为狗肉、羊肉、鸭肉(CP 31%).试验期为4周.结果表明:饲喂狗肉和羊肉能显著提高大鼠生长性能,且前者使得大鼠血清碱性磷酸酶(ALP)活性极显著升高(P<0.01);而饲喂鸭肉使得大鼠血清丙氨酸转移酶(ALT)活性显著升高(P<0.05),天冬氨酸转移酶(AST)、血清尿素氮(BUN)活性极显著升高(P<0.01).不同属种的肉之间,鸭肉组肝脏和肾脏中谷胱甘肽过氧化物酶(GSH-Px)活性均极显著升高(P<0.01).结果提示:饲喂狗肉和羊肉能促进机体蛋白质合成,有利于蛋白质沉积;饲喂鸭肉则具有增强机体抗氧化的能力.%The experiment was conducted to study the effect of different species meats on serum biochemical parameters and antioxidant status in rats. A total of 90 male SD rats with body weight of about 177 g were divided into dog meat group, mutton group and duck meat group in a randomized complete block design, and these three groups were respectively replaced the main dietary protein source with dog meat. mutton, duck meat ( CP% : 31%). The experimental stage was 4 weeks. The results were shown as follows : the growth performance of rats was markedly enhanced by feeding dog meat and mutton, and the activity of alkaline phosphatase ( ALP) of rats in dog meat group was enhanced significandy ( P<0. 01). But with the rats in duck meat group, the activity of alanine aminotransferase ( ALT) was increased significantly (P<0. 05) . the activity of aspartate aminotransferase ( AST) and serum urea nitrogen ( BUN) was enhanced significantly (P<0. 01). Among the groups of different species meats, the activity of glutathione peroxidase ( GSH) in liver and kidney of rats in duck meat group was significantly higher ( P<0. 01). The

  11. A study on the effects of amphoteric defect concentration on the characteristics parameters of In x Ga1- x N thin-film solar cells

    Science.gov (United States)

    Movla, Hossein; Babazadeh, Mohammad; Esmaeili, Seyed Vahid

    2016-07-01

    Group III nitride semiconductors can partly cover the solar spectrum from ultraviolet to infrared spectra due to their ability to vary their band gap. These semiconductors have a substantial potential to develop ultra-high efficiency solar cells. However, defects have a profound effect on their power conversion efficiency. Since defects lead to dramatic changes in electronic and optoelectronic properties, controlling process to get acceptable defects density in solar cells is a noteworthy parameter in technological and device applications. This paper indicates a numerical simulation study to optimize the p-i-n InGaN homojunction solar cells by investigation of defect density in the whole cell structure. In this study, we assumed that the p-region and n-region thicknesses are 100 and 150 nm, respectively, and the optimized value of cell thickness is 1.3 μm. Similarly, we chose amphoteric defect density from 1015 to 1019 cm-3, and then the effects of defects density on characteristic parameters of cell have been studied. Based on our results, when the amphoteric defect concentration is below the 1015 cm-3, constant value for FF values in all layers was obtained. Therefore, cell efficiency remains the same in lower amphoteric defect density where all FF, V OC and J SC are constant. By increasing the amphoteric defect density from 1015 cm-3, the cell efficiency falls down dramatically from 20 to about 1 % at 1019 cm-3. In our simulated structure, the cell efficiency parameter decreases with increasing the defects density until it reaches the 1015 cm-3. Above this value, no change in the parameters was observed. Our results revealed that the high defect density range 1015-1019 cm-3 may be an equally significant cause of performance loss.

  12. Effect of a mixture of micronutrients, but not of bovine colostrum concentrate, on immune function parameters in healthy volunteers: a randomized placebo-controlled study

    Directory of Open Access Journals (Sweden)

    van der Wielen Reggy PJ

    2006-11-01

    Full Text Available Abstract Background Supplementation of nutritional deficiencies helps to improve immune function and resistance to infections in malnourished subjects. However, the suggested benefits of dietary supplementation for immune function in healthy well nourished subjects is less clear. Among the food constituents frequently associated with beneficial effects on immune function are micronutrients such as vitamin C, vitamin E, β-carotene and zinc, and colostrum. This study was designed to investigate the effects these ingredients on immune function markers in healthy volunteers. Methods In a double-blind, randomized, parallel, 2*2, placebo-controlled intervention study one hundred thirty-eight healthy volunteers aged 40–80 y (average 57 ± 10 y received one of the following treatments: (1 bovine colostrum concentrate 1.2 g/d (equivalent to ~500 mg/d immunoglobulins, (2 micronutrient mix of 288 mg vitamin E, 375 mg vitamin C, 12 mg β-carotene and 15 mg zinc/day, (3 combination of colostrum and micronutrient mix, or (4 placebo. Several immune function parameters were assessed after 6 and 10 weeks. Data were analyzed by analysis of variance. Groups were combined to test micronutrient treatment versus no micronutrient treatment, and colostrum treatment versus no colostrum treatment. Results Overall, consumption of the micronutrient mix significantly enhanced delayed-type hypersensitivity (DTH responses (p Conclusion Consumption of bovine colostrum had no effect on any of the immune parameters assessed. The micronutrient mix enhanced cellular immunity as measured by DTH, with an increased effect by incremental age, but did not affect any of the other immune parameters measured. Although correlations between decreased DTH and enhanced risk of certain infection have been reported, it remains unclear whether and enhanced DTH response actually improves immune defense. The present data suggests that improvement of immune parameters in a population with a

  13. 新疆石河子地区奶牛血液生化指标正常参考值范围的建立%Establishment of the Normal Reference Range of Blood Biochemical Parameters for Dairy Cow in Shihezi Area of Xinjiang

    Institute of Scientific and Technical Information of China (English)

    李新萍; 陶岳; 张孝恩; 林为民; 史文军; 陈红; 叶丹; 王宏伟

    2011-01-01

    为建立符合石河子地区奶牛血液生化特点的正常参考值范围,随机抽取石河子地区莫索湾、安集海、下野地等地犊牛及成年奶牛血清共计933份(其中成年奶牛818份、犊牛115份)进行23项生化指标检测。用SPSS软件进行统计分析,确立生化指标正常参考值范围。结果显示,石河子地区奶牛血液生化指标正常值参考范围存在年龄间差异,与国外提供的参考值相比各指标有一定的差异。%To establish a reference range of blood biochemical parameters for Shihezi dairy cow, 933 blood samples(818 adult cows and 115 calves)were collected from Mosuowan, Anjihai and Xiayedi in Shihezi to detect 23 kinds of biochemical parameters. SPSS software was used for statistical analysis. The resuhs showed that the normal reference range of blood biochemical for dairy cow in Shihezi had differences among ages. Compared with the abroad reference values, there were also some differences.

  14. Recent abstracts in biochemical technology

    OpenAIRE

    R R Siva Kiran; Brijesh P

    2008-01-01

    “Recent abstracts in biochemical technology” is a collection of interesting research articles published in “List of biochemical technology journals” (Table 1). The abstracts are most likely to report significant results in biochemical technology.

  15. Thyroid-Stimulating Hormone, Triiodotyronine and Thyroxine Concentrations and Their Relationship with Metabolic Parameters, Anthropometric Variables and Body Composition in Premenopausal Euthyroid Obese Women

    Directory of Open Access Journals (Sweden)

    Mahdieh Abbasalizad Farhangi

    2015-03-01

    Full Text Available Purpose: This study was performed to evaluate the potential relationships of thyroid hormones, metabolic parameters, and anthropometric variables with body composition in premenopausal women. Material and Method: A total of 84 women with a mean age of 35.12±8.11 years were investigated. Subjects with a history of diabetes, hyperthyroidism and hypothyroidism, chronic liver, and renal disease were excluded from the study. In all subjects, anthropometric parameters were evaluated and body composition was analyzed by bioelectrical impedance analysis (BIA. Fasting serum thyroid-stimulating hormone (TSH, triiodothyrionine (T3, thyroxine (T4, fasting blood glucose (FBG, total cholesterol (TC, triglyceride (TG, high-density lipoprotein cholesterol (HDL-C, C-reactive protein (CRP, and interleukin 6 (IL-6 were measured by enzymatic methods. Serum low-density lipoprotein cholesterol (LDL-C was determined by Friedewald formula. Results: Serum TSH, CRP and TC levels in obese subjects were significantly higher than in non-obese subjects (p<0.05. Serum TSH concentration was positively associated with waist to hip ratio (WHR and fat mass and, negatively associated with fat-free mass (p<0.05. There was also a positive relationship between T3 and TG and between T4 and LDL-C in all participants. In comparison of subjects with different body fat percentage, subjects with higher body fat had also higher amount of TSH concentrations (p=0.015. Discussion: Our results showed a relationship of fat mass and lipid profile with thyroid function in premenopausal women. Further researches are needed to clarify the nature and mechanism of these relationships.

  16. INFLUENCE OF LIFETIME EXPOSURE OF SUBLETHAL DOSES OF CADMIUM TO SELECTED PARAMETERS OF CARBOHYDRATE METABOLISM

    OpenAIRE

    František Ništiar; Iveta Cimboláková; Eva Lovásová; Jaroslava Nováková; Agnesa Lukačínová

    2012-01-01

    The aim of the study was to assess the effects of exposure to low doses of cadmium dissolved in drinking water (at a concentration 200 times higher than the maximum permissible dose) on selected parameters of carbohydrate metabolism in 20 Wistar rats. Animals were divided into two groups – control and experimental groups exposed to low doses of cadmium chloride in concentration 20 μM of drinking water. We studied the biochemical parameters, as glucose, hemoglobin, glycated hemoglobin...

  17. Analysis of industrial impact on physiochemical parameters and heavy metal concentrations in waters of river Majidun, Molatori and Ibeshe around Ikorodu in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    O.E. Bolawa

    2012-03-01

    Full Text Available The pollution of aquatic ecosystems by heavy metals has assumed serious proportion due to their toxicity and accumulative behavior. Various hydrochemical parameters of river water (pH, total dissolved solid, sulfate, nitrate, calcium, magnesium in these rivers around Ikorodu were studied in 2005. The main aim of this study is to assess the impact of effluent discharge on the water quality of rivers Majidun, Molatori and Ibeshe. Physiochemical parameters including pH, total solids, total dissolved solids, alkalinity and nitrate were used for the evaluation of effluent discharge on the rivers. The results were compared with standard permissible limits. The concentrations of cadmium, chromium, copper, iron, lead, manganese and magnesium were also determined in the water samples. Results obtained show the pollution tendencies of the rivers. This was attributed to high levels of lead, chromium, and cadmium. The results also showed that with the continuous discharge of industrial effluents, pollution of the rivers by heavy metals is likely to have adverse effects.

  18. Performance, intestinal histomorphometry, and blood parameters of post-weaning piglets receiving different levels of soy protein concentrate in the diet

    Directory of Open Access Journals (Sweden)

    Moisés Queiroz Resende

    2016-06-01

    Full Text Available ABSTRACT Seventy-two barrows were used to evaluate the effects of the use of soy protein concentrate (SPC in diets of post-weaning piglets on their performance, intestinal histomorphometry, and blood parameters. Piglets were weaned at 21 days of age and distributed in a randomized-block experimental design with four treatments (0, 3, 6, and 9% SPC, six replications, and three piglets per replication. Diets were formulated to meet the requirement of the piglets according to phases of 21 to 32, 33 to 42, and 43 to 66 days of age. Both feed and water were provided ad libitum during the entire experiment. The feed of the phase of 43 to 66 days was based on corn and soybean meal. In the period between 21 and 32 days of age, the use of SPC in the diet did not impact the daily weight gain (DWG or the daily feed intake (DFI of the piglets, whereas there was a linear effect on feed conversion (FC, which decreased as the levels of SPC in the feed were increased. In the period between 33 and 42 days of age, a linear effect was observed on FC, which increased as the level of SPC in the feed was increased. In this period, no effects of SPC were observed on DWG or DFI. Inclusion of SPC in the diets does not influence performance, intestinal histomorphometry, or blood parameters of piglets in the period between 21 and 66 days of age.

  19. Growth parameters and resistance against Drechslera teres of spring barley (Hordeum vulgare L. cv. Scarlett) grown at elevated ozone and carbon dioxide concentrations.

    Science.gov (United States)

    Plessl, M; Heller, W; Payer, H-D; Elstner, E F; Habermeyer, J; Heiser, I

    2005-11-01

    Spring barley ( Hordeum vulgare L. cv. Scarlett) was grown at two CO2 levels (400 vs. 700 ppm) combined with two ozone regimes (ambient vs. double ambient) in climate chambers for four weeks, beginning at seedling emergence. Elevated CO2 concentration significantly increased aboveground biomass, root biomass, and tiller number, whereas double ambient ozone significantly decreased these parameters. These ozone-induced reductions in growth parameters were strongly overridden by 700 ppm CO2. The elevated CO2 level increased C : N ratio of the leaf tissue and leaf starch content but decreased leaf protein levels. Exposure to double ambient ozone did not affect protein content and C : N ratio but dramatically increased leaf starch levels at 700 ppm CO2. Resistance against Drechslera teres (Sacc.) Shoemaker was increased in leaves grown at double ambient ozone but was less obvious at 700 ppm than at 400 ppm CO2. Constitutive activities of beta-1,3-glucanase and chitinase were significantly higher in leaves grown at double ambient ozone compared to ambient ozone levels. The sum of methanol-soluble and alkali-released cell wall-bound aromatic metabolites (i.e., C-glycosylflavones and several structurally unidentified metabolites) and lignin contents did not show any treatment-dependent differences.

  20. Modeling and Characteristic Parameters Analysis of a Trough Concentrating Photovoltaic/Thermal System with GaAs and Super Cell Arrays

    Directory of Open Access Journals (Sweden)

    Xu Ji

    2012-01-01

    Full Text Available The paper established the one-dimension steady models of a trough concentrating photovoltaic/thermal system with a super cell array and a GaAs cell array, respectively, and verified the models by experiments. The gaps between calculation results and experimental results were less than 5%. Utilizing the models, the paper analyzed the influences of the characteristic parameters on the performances of the TCPV/T system with a super cell array and a GaAs cell array, respectively. The reflectivity of the parabolic mirror in the TCPV/T system was an important factor to determine the utilizing efficiency of solar energy. The performances of the TCPV/T system can be optimized by improving the mirror reflectivity and the thermal solar radiation absorptivity of the lighting plate and pursuing a suitable focal line with uniform light intensity distribution. All these works will benefit to the utilization of the trough concentrating system and the combined heat/power supply.

  1. Seasonal and temporal variations of criteria air pollutants and the influence of meteorological parameters on the concentration of pollutants in ambient air in lahore, pakistan

    International Nuclear Information System (INIS)

    Criteria air pollutants have their significance for causing health threats and damage to the environment. The study was conducted to assess the seasonal and temporal variations of criteria air pollutants and evaluating the correlations of criteria air pollutants with meteorological parameters in the city of Lahore, Pakistan for a period of one year from April 2010 to March 2011. The concentrations of criteria air pollutants were determined at fixed monitoring stations equipped with HORIBA analyzers. The annual average concentrations (μ/m/super 3/) of PM /sub 2.5/, O/sub 3/, SO/sub 2/, CO and NO/sub x/ (NO+NO/sub 2/) for this study period were 118.94±57.46, 46.0±24.2, 39.9±8.9, 1940±1300 and 130.9±81.0 (61.8±46.2+57.3±22.19), respectively. PM/sub 2.5/, SO/sub 2/, CO and NO/sub x/ had maximum concentrations during winter whereas O/sub 3/ had maximum concentration during summer. Minimum concentrations of PM/sub 2.5/, SO/sub 2/ and NO/sub x/ were found during monsoon as compared to other seasons due to rainfall which scavenged these pollutants. The O/sub 3/ showed positive correlation with temperature and solar radiation but negative correlation with wind speed. All other criteria air pollutants showed negative correlation with wind speed, temperature and solar radiation. A significant (P<0.01) correlation was found between NO/sub x/ and CO (r = 0.779) which showed that NO/sub x/ and CO arise from common source that could be the vehicular emission. PM/sub 2.5/ was significantly correlated (P<0.01) with NO/sub x/ (r = 0.524) and CO (r = 0.519), respectively. High traffic intensity and traffic jams were responsible for increased air pollutants level especially the PM/sub 2.5/, NO/sub x/ and CO. (author)

  2. Hematological and biochemical parameters of Dorper ewes
    Parâmetros hematológicos e bioquímicos de ovinos da raça Dorper

    OpenAIRE

    Karina Medici Madureira; Viviani Gomes; Brenda Barcelos; Breno Henrique Zani; Carolina de Lara Shecaira; Camila Ramos Costa; Fernando José Benesi

    2013-01-01

    In order to establish hematological and biochemical Dorper sheep values and evaluate the influence of sex and age in these determinations, 72 animals were assigned to three experimental groups according to age: Group 1 (G1) consisting of animals up to 12 months of age (n=28), Group 2 (G2) composed of animals from 13 to 36 months (n=37) and Group 3 (G3) for animals from 37 to 60 months (n=7). The animals were still divided in another two groups according to gender: Group 4 (G4) – females (n=57...

  3. Characterizing multistationarity regimes in biochemical reaction networks.

    Directory of Open Access Journals (Sweden)

    Irene Otero-Muras

    Full Text Available Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space.

  4. DEVELOPMENT OF WATER QUALITY PARAMETER RETRIEVAL ALGORITHMS FOR ESTIMATING TOTAL SUSPENDED SOLIDS AND CHLOROPHYLL-A CONCENTRATION USING LANDSAT-8 IMAGERY AT POTERAN ISLAND WATER

    Directory of Open Access Journals (Sweden)

    N. Laili

    2015-10-01

    Full Text Available The Landsat-8 satellite imagery is now highly developed compares to the former of Landsat projects. Both land and water area are possibly mapped using this satellite sensor. Considerable approaches have been made to obtain a more accurate method for extracting the information of water area from the images. It is difficult to generate an accurate water quality information from Landsat images by using some existing algorithm provided by researchers. Even though, those algorithms have been validated in some water area, but the dynamic changes and the specific characteristics of each area make it necessary to get them evaluated and validated over another water area. This paper aims to make a new algorithm by correlating the measured and estimated TSS and Chla concentration. We collected in-situ remote sensing reflectance, TSS and Chl-a concentration in 9 stations surrounding the Poteran islands as well as Landsat 8 data on the same acquisition time of April 22, 2015. The regression model for estimating TSS produced high accuracy with determination coefficient (R2, NMAE and RMSE of 0.709; 9.67 % and 1.705 g/m3 respectively. Whereas, Chla retrieval algorithm produced R2 of 0.579; NMAE of 10.40% and RMSE of 51.946 mg/m3. By implementing these algorithms to Landsat 8 image, the estimated water quality parameters over Poteran island water ranged from 9.480 to 15.801 g/m3 and 238.546 to 346.627 mg/m3 for TSS and Chl-a respectively.

  5. Controlling Foam Morphology of Poly(methyl methacrylate via Surface Chemistry and Concentration of Silica Nanoparticles and Supercritical Carbon Dioxide Process Parameters

    Directory of Open Access Journals (Sweden)

    Deniz Rende

    2013-01-01

    Full Text Available Polymer nanocomposite foams have received considerable attention because of their potential use in advanced applications such as bone scaffolds, food packaging, and transportation materials due to their low density and enhanced mechanical, thermal, and electrical properties compared to traditional polymer foams. In this study, silica nanofillers were used as nucleating agents and supercritical carbon dioxide as the foaming agent. The use of nanofillers provides an interface upon which CO2 nucleates and leads to remarkably low average cell sizes while improving cell density (number of cells per unit volume. In this study, the effect of concentration, the extent of surface modification of silica nanofillers with CO2-philic chemical groups, and supercritical carbon dioxide process conditions on the foam morphology of poly(methyl methacrylate, PMMA, were systematically investigated to shed light on the relative importance of material and process parameters. The silica nanoparticles were chemically modified with tridecafluoro-1,1,2,2-tetrahydrooctyl triethoxysilane leading to three different surface chemistries. The silica concentration was varied from 0.85 to 3.2% (by weight. The supercritical CO2 foaming was performed at four different temperatures (40, 65, 75, and 85°C and between 8.97 and 17.93 MPa. By altering the surface chemistry of the silica nanofiller and manipulating the process conditions, the average cell diameter was decreased from 9.62±5.22 to 1.06±0.32 μm, whereas, the cell density was increased from 7.5±0.5×108 to 4.8±0.3×1011 cells/cm3. Our findings indicate that surface modification of silica nanoparticles with CO2-philic surfactants has the strongest effect on foam morphology.

  6. Development of Water Quality Parameter Retrieval Algorithms for Estimating Total Suspended Solids and Chlorophyll-A Concentration Using LANDSAT-8 Imagery at Poteran Island Water

    Science.gov (United States)

    Laili, N.; Arafah, F.; Jaelani, L. M.; Subehi, L.; Pamungkas, A.; Koenhardono, E. S.; Sulisetyono, A.

    2015-10-01

    The Landsat-8 satellite imagery is now highly developed compares to the former of Landsat projects. Both land and water area are possibly mapped using this satellite sensor. Considerable approaches have been made to obtain a more accurate method for extracting the information of water area from the images. It is difficult to generate an accurate water quality information from Landsat images by using some existing algorithm provided by researchers. Even though, those algorithms have been validated in some water area, but the dynamic changes and the specific characteristics of each area make it necessary to get them evaluated and validated over another water area. This paper aims to make a new algorithm by correlating the measured and estimated TSS and Chla concentration. We collected in-situ remote sensing reflectance, TSS and Chl-a concentration in 9 stations surrounding the Poteran islands as well as Landsat 8 data on the same acquisition time of April 22, 2015. The regression model for estimating TSS produced high accuracy with determination coefficient (R2), NMAE and RMSE of 0.709; 9.67 % and 1.705 g/m3 respectively. Whereas, Chla retrieval algorithm produced R2 of 0.579; NMAE of 10.40% and RMSE of 51.946 mg/m3. By implementing these algorithms to Landsat 8 image, the estimated water quality parameters over Poteran island water ranged from 9.480 to 15.801 g/m3 and 238.546 to 346.627 mg/m3 for TSS and Chl-a respectively.

  7. Effect of Key Parameters on the Photocatalytic Oxidation of Toluene at Low Concentrations in Air under 254 + 185 nm UV Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Quici, Natalia; Vera, Maria L.; Choi, Hyeok; Puma, Gianluca Li; Dionysiou, Dionysios D.; Litter, Marta I.; Destaillats, Hugo

    2009-07-01

    The effect of key experimental parameters on the removal of toluene under 254 + 185 nm irradiation was investigated using a benchtop photocatalytic flow reactor. Toluenewas introduced at low concentrations between 10 and 500 ppbv, typical of indoorenvironments, and reacted on TiO2-coated Raschig rings. Two different TiO2-coated rings were prepared: in one case, by dip-coating using a P25 aqueous suspension and, on the other, using an organic/inorganic sol-gel method that produced thin films of mesoporous anatase. Flow rates in the photoreactor varied between 4 L min-1 and 125 mL min-1, leading to residence times in the range 100 ms< tau< 2 s. For these conditions, toluene removal efficiencies were between 30 and 90percent, indicating that the system did not achieve total conversion in any case. For each air flow rate, the conversion oftoluene was significantly higher when the reactor length was 10 cm, as compared with 5 cm; however, only marginal increases in conversions were achieved in the two reactor lengths at equal residence time and different concentration of toluene, suggesting that that the reactor is effectively behaving as an ideal reactor and that the reaction is first-order in the concentration of toluene. Experiments were carried out between 0 and 66percent relative humidity (RH), the fastest reaction rate being observed at moderately low humidity conditions (10percent RH), with respect to both dry air and higher humidity levels. Formaldehyde was formed as a partial oxidation byproduct at low and at high residence times (240 and 960 ms), although higher formaldehyde molar yields (up to 20percent) were observed at low tau (240 ms) and moderate humidity conditions (10 and 33percent), suggesting that both tau and RH can be optimized toreduce the formation of harmful intermediates. Toluene removal efficiency increased with the TiO2 thickness (i.e., mass) until a maximum value of 500 nm, beyond which the removal efficiency decreased. This should be

  8. 融水小型猪 F1代的血常规、血生化与电解质指标%Detection and analysis of blood parameter, blood biochemical and electrolyte indices in F1 generation of Rongshui miniature pig

    Institute of Scientific and Technical Information of China (English)

    刘科; 唐小江; 施赫赫; 陈淦; 邝少松; 刘盛来; 刘运忠; 钟志勇; 郭金彪; 耿庆山

    2015-01-01

    Objective To detect the blood parameter, blood biochemical and electrolyte indices of the F1 generation of Rongshui miniature pig ( RMP) .Methods The blood of 43 female and 42 male RMPs of 4 th month old, and 36 RMPs of 12th month old ( half male and female) were extracted from jugular vein.And the blood parameter, blood biochemical and electrolyte indices were detected by blood analyzer and automatic biochemical analyzer.Results In the same month-old RMP, no significant difference between male and female were found in most indices of blood parameter, blood biochemical and electrolyte indices.On the other hand, many indices were difference between 4th month old and 12th month old RMPs of same gender.Compared with the 4th month old RMP, the 12th month old RMP decreased significantly in WBC and PLT, increased in HGB ( P 0.05 ) .Serum ALT, AST, ALP, CK (male), LDH(male), A/G, BUN, GLU (female), CHOL (male) and K+decreased significantly (P <0.05), while serum TP, TBIL, CR and Ca2+increased significantly (P <0.05),but serum CHOL, TG, HDL-C and LDL-C were not different.86.4%(19/22) biochemical and electrolyte indices in RMP were in/or close to the range of normal value of human.Conclusion Most of the blood parameter, blood biochemical and electrolyte indices of RMP were close to human’ s normal value.%目的:测定融水小型猪( rongshui miniature pig,RMP) F1代血常规、血生化与电解质指标。方法选取4月龄F1代RMP 85头,雌性43头、雄性42头;12月龄36头,雌雄各半。颈静脉采血,分析血常规、血生化与电解质等22项指标。结果融水小型猪相同月龄时,两性别的血常规指标、血生化和电解质的大部分指标差异不大,但同性别在不同月龄之间的部分指标差异明显。12月龄比4月龄融水小型猪,血常规指标中的白细胞数、血小板数下降,血红蛋白含量升高( P <0.05),而红细胞数变化不大( P >0.05);血清ALT、AST、ALP、CK

  9. Measures of Biochemical Sociology

    Science.gov (United States)

    Snell, Joel; Marsh, Mitchell

    2008-01-01

    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  10. Biochemical Education in Brazil.

    Science.gov (United States)

    Vella, F.

    1988-01-01

    Described are discussions held concerning the problems of biochemical education in Brazil at a meeting of the Sociedade Brazileira de Bioquimica in April 1988. Also discussed are other visits that were made to universities in Brazil. Three major recommendations to improve the state of biochemistry education in Brazil are presented. (CW)

  11. Biochemical Markers of Myocardial Damage.

    Science.gov (United States)

    Bodor, Geza S

    2016-04-01

    Heart diseases, especially coronary artery diseases (CAD), are the leading causes of morbidity and mortality in developed countries. Effective therapy is available to ensure patient survival and to prevent long term sequelae after an acute ischemic event caused by CAD, but appropriate therapy requires rapid and accurate diagnosis. Research into the pathology of CAD have demonstrated the usefulness of measuring concentrations of chemicals released from the injured cardiac muscle can aid the diagnosis of diseases caused by myocardial ischemia. Since the mid-1950s successively better biochemical markers have been described in research publications and applied for the clinical diagnosis of acute ischemic myocardial injury. Aspartate aminotransferase of the 1950s was replaced by other cytosolic enzymes such as lactate dehydrogenase, creatine kinase and their isoenzymes that exhibited better cardiac specificity. With the availability of immunoassays, other muscle proteins, that had no enzymatic activity, were also added to the diagnostic arsenal but their limited tissue specificity and sensitivity lead to suboptimal diagnostic performance. After the discovery that cardiac troponins I and T have the desired specificity, they have replaced the cytosolic enzymes in the role of diagnosing myocardial ischemia and infarction. The use of the troponins provided new knowledge that led to revision and redefinition of ischemic myocardial injury as well as the introduction of biochemicals for estimation of the probability of future ischemic myocardial events. These markers, known as cardiac risk markers, evolved from the diagnostic markers such as CK-MB or troponins, but markers of inflammation also belong to these groups of diagnostic chemicals. This review article presents a brief summary of the most significant developments in the field of biochemical markers of cardiac injury and summarizes the most recent significant recommendations regarding the use of the cardiac markers in

  12. Vector Encoding in Biochemical Networks

    Science.gov (United States)

    Potter, Garrett; Sun, Bo

    Encoding of environmental cues via biochemical signaling pathways is of vital importance in the transmission of information for cells in a network. The current literature assumes a single cell state is used to encode information, however, recent research suggests the optimal strategy utilizes a vector of cell states sampled at various time points. To elucidate the optimal sampling strategy for vector encoding, we take an information theoretic approach and determine the mutual information of the calcium signaling dynamics obtained from fibroblast cells perturbed with different concentrations of ATP. Specifically, we analyze the sampling strategies under the cases of fixed and non-fixed vector dimension as well as the efficiency of these strategies. Our results show that sampling with greater frequency is optimal in the case of non-fixed vector dimension but that, in general, a lower sampling frequency is best from both a fixed vector dimension and efficiency standpoint. Further, we find the use of a simple modified Ornstein-Uhlenbeck process as a model qualitatively captures many of our experimental results suggesting that sampling in biochemical networks is based on a few basic components.

  13. The Correlation study on the production performance and blood biochemical parameters of Big bone chicken%大骨鸡血液生化指标与生产性能的相关性研究

    Institute of Scientific and Technical Information of China (English)

    陈宁; 郭丹; 门德才

    2014-01-01

    本研究利用3个血液酶活性标记对庄河大骨鸡的血液生化指标多态性进行研究,并与大骨鸡的产肉性能和产蛋性能进行相关性分析,筛选出用于大骨鸡产肉性能的优势遗传标记2个,产蛋性能的优势遗传标记1个,产蛋产肉兼用的优势遗传标记1个。%In this study, the polymorphism of blood biochemical indexes was investigated by the activities of three blood enzyme in the Zhuanghe bone chicken, and the correlation analysis be-tween meat and egg production performances were conducted in order to screen for two advantageous genetic markers of meat production and one advantageous genetic marker of egg production, one ad-vantageous genetic marker of both meat and egg production marker.

  14. Reconfigurable neuromorphic computation in biochemical systems.

    Science.gov (United States)

    Chiang, Hui-Ju Katherine; Jiang, Jie-Hong R; Fages, Francois

    2015-08-01

    Implementing application-specific computation and control tasks within a biochemical system has been an important pursuit in synthetic biology. Most synthetic designs to date have focused on realizing systems of fixed functions using specifically engineered components, thus lacking flexibility to adapt to uncertain and dynamically-changing environments. To remedy this limitation, an analog and modularized approach to realize reconfigurable neuromorphic computation with biochemical reactions is presented. We propose a biochemical neural network consisting of neuronal modules and interconnects that are both reconfigurable through external or internal control over the concentrations of certain molecular species. Case studies on classification and machine learning applications using the DNA strain displacement technology demonstrate the effectiveness of our design in both reconfiguration and autonomous adaptation. PMID:26736417

  15. Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758: Biochemical response

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2014-01-01

    Full Text Available Chlorpyrifos, an organophosphate pesticide, is widely used to control pests in agriculture farms and orchards of fruit trees. In this study, the fish were exposed to sub-lethal concentrations of chlorpyrifos which were determined based on numerical value of 96 h LC50. Blood was sampled after 10, 20 and 30 days and biochemical parameters including glucose, total protein, albumin, globulin, triglyceride and cholesterol levels, and aspartate aminotransferase (AST, alanine aminotransferase (ALT, lactate dehydrogenase (LDH, creatine kinase (CK, alkaline phosphatase (ALP and acetylcholinsetrase (AChE activities were measured. Behavioral changes in the fish were also recorded during the experiment. Unbalanced swimming, swimming in the surface water and hyperglycemia, increased blood triglyceride, and increased levels of AST, LDH and CK activities as well as decreased levels of AChE activity were important changes that were observed in the specimens exposed to chlorpyrifos during experimental periods. The most important alterations in the blood biochemical parameters were measured in the specimens exposed to 40 µg/L chlorpyrifos on the 20th and 30th day of the trial. In conclusion, results of the present study indicated that exposure to sub-lethal concentrations of chlorpyrifos as low as 40 µg/L may cause biochemical and behavioral changes in Cyprinus carpio.

  16. Early biochemical biomarkers for zinc in silver catfish (Rhamdia quelen) after acute exposure.

    Science.gov (United States)

    Leitemperger, Jossiele; Menezes, Charlene; Santi, Adriana; Murussi, Camila; Lópes, Thais; Costa, Maiara; Nogueira, Lygia Sega; Loro, Vania Lucia

    2016-06-01

    Contamination of aquatic ecosystems by metals causes various biochemical changes in aquatic organisms, and fish are recognized as indicators of environmental quality. Silver catfish were exposed to six concentrations of zinc (Zn): 1.0, 2.5, 5.0, 7.5, 10.0 and 12.5 mg/L for 96 h to determine the mean lethal concentration (LC50). The value obtained was 8.07 mg/L. In a second experiment, fish were exposed to concentrations of 1.0 or 5.0 mg/L Zn and a control for 96 h. Afterward, the tissues were collected for biochemical analysis. Lipid peroxidation, as indicated by thiobarbituric acid-reactive substance (TBARS), decreased in the liver and brain for all Zn concentrations tested, while in the gills TBARS levels increased at 1.0 mg/L and declined at 5.0 mg/L. Zn increased protein carbonyls in the muscle of silver catfish and decreased it in the other tissues. The enzyme superoxide dismutase increased in both exposed groups. However, catalase did not change. Glutathione S-transferase decreased in the liver and increased in the gills (1.0 mg/L), muscle (5.0 mg/L) and brain (1.0 and 5.0 mg/L). Nonprotein thiols changed only in brain and muscle tissue. Zn exposure inhibited acetylcholinesterase (AChE) activity in the brain at both concentrations tested, but did not change it in muscle. Exposure to Zn inhibited the activity of Na(+)/K(+)-ATPase in the gills and intestine at both concentrations tested. Our results demonstrate that Zn alters biochemical parameters in silver catfish and that some parameters such as AChE and Na(+)/K(+)-ATPase could be considered as early biomarkers of waterborne Zn toxicity. PMID:26744269

  17. Using combined measurements of gas exchange and chlorophyll fluorescence to estimate parameters of a biochemical C3 photosynthesis model: a critical ap