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Sample records for biochemical parameters concentration

  1. Changes in serum mineral concentrations, biochemical and hematological parameters in horses with pica.

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    Aytekin, Ismail; Onmaz, Ali Cesur; Aypak, Serap Unubol; Gunes, Vehbi; Kucuk, Osman

    2011-03-01

    The aim of this study was to compare hematological, some biochemical parameters, and serum trace element concentrations in horses with or without pica. Fifteen horses with pica (group I) and another 15 healthy horses without pica (group II) were used. The hematological parameters were not changed between the two groups. In group I, hemoglobin values were lower than those of group II. However, the difference in hemoglobin values between the two groups was not significant (P > 0.05). Serum iron and copper concentrations and the copper/zinc ratio were lower in group I than those of group II (P  0.05). It was concluded that serum iron and copper deficiency may play an important role for the etiology of pica in horses. Prophylactic use of iron and copper supplements in horses may be beneficial to prevent pica.

  2. The Effects of Air Pollution and Smoking on Cadmium Concentration in Human Blood and Correlation with Biochemical Parameters

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    L. Zeneli

    2009-01-01

    Full Text Available Problem statement: The study described the research of the effects that the environment pollution and smoking have in cadmium concentration in human blood, as well as in the correlation between cadmium and the biochemical parameters. Approach: In a comparative study of cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants (Obiliq, a highly polluted environments (Investigated Group and the other that was considered as relatively clean rural environment Dragash (control group. Results: The results showed that there exists a significant difference in the average concentration of cadmium in human blood between the Investigated Group (IG and the Control Group (CG (t = -3.34, p = 0.0006. The series of determination of cadmium concentration in blood of population that lives in this environment had shown direct effects in biochemical parameters (direct bilirubine, total bilirubine. Conclusion: Air pollution (from coal burning in power plant and smoking were very important factors for the level of cadmium concentration in blood, which had an inhibitory effect in the syntheses of bilirubine.

  3. Haematological and serum biochemical parameters of West African Dwarf goats fed dried cassava leaves-based concentrate diets.

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    Oni, Adebayo Olusoji; Arigbede, Oluwasanmi Moses; Sowande, Olusiji Sunday; Anele, Uchenna Young; Oni, Oluwakemi Oluremilekun; Onwuka, Chryss Friday Ijeoma; Onifade, Olufemi Sunday; Yusuf, Kafayat Omowumi; Dele, Peter Aniwe; Aderinboye, Ronke Yemisi

    2012-03-01

    The study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of West African Dwarf (WAD) goats. The study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. At the start of the experiment, packed cell volume (PCV) ranged from 21.5% to 25.5% while haemoglobin concentration (Hb) and RBC significantly (P diets. At the end of the trial, there was a slight increase in the values of PCV and Hb in the diets (P > 0.05). Lymphocyte reduced significantly (P diets. Neutrophils, however, increased (P > 0.05) at the 0% to 40% levels and reduced at the 60% level of dried cassava leaves inclusion. At the start of the experiment, values for glucose significantly (P diets. The study revealed that inclusion of dried cassava leaves in the diets of West African Dwarf goats had no deleterious effects on the haematological and serum biochemical parameters of WAD goats and could therefore be included in ruminant diets up to 60%.

  4. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats.

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    Piątkowska, Ewa; Kopeć, Aneta; Bieżanowska-Kopeć, Renata; Pysz, Mirosław; Kapusta-Duch, Joanna; Koronowicz, Aneta Agnieszka; Smoleń, Sylwester; Skoczylas, Łukasz; Ledwożyw-Smoleń, Iwona; Rakoczy, Roksana; Maślak, Edyta

    2016-01-01

    Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues and various biochemical parameters as well as mRNA expression of some genes involved in iodine metabolism in Wistar rats. Statistically, a significantly higher iodine level was determined in urine, faeces and selected tissues of rats fed a diet containing biofortified raw carrot as compared to a diet without iodine and a diet containing control cooked carrot. Biofortified raw carrot significantly increased triiodothyronine concentration as compared to animals from other experimental groups. The highest thyroid stimulating hormone level was determined in rats fed control cooked carrots. mRNA expression of selected genes was affected by different dietary treatment in rats' hearts. Biofortified raw and cooked carrot could be taken into account as a potential source of iodine in daily diets to prevent iodine deficiency in various populations.

  5. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats.

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    Ewa Piątkowska

    Full Text Available Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues and various biochemical parameters as well as mRNA expression of some genes involved in iodine metabolism in Wistar rats. Statistically, a significantly higher iodine level was determined in urine, faeces and selected tissues of rats fed a diet containing biofortified raw carrot as compared to a diet without iodine and a diet containing control cooked carrot. Biofortified raw carrot significantly increased triiodothyronine concentration as compared to animals from other experimental groups. The highest thyroid stimulating hormone level was determined in rats fed control cooked carrots. mRNA expression of selected genes was affected by different dietary treatment in rats' hearts. Biofortified raw and cooked carrot could be taken into account as a potential source of iodine in daily diets to prevent iodine deficiency in various populations.

  6. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats

    OpenAIRE

    Ewa Piątkowska; Aneta Kopeć; Renata Bieżanowska-Kopeć; Mirosław Pysz; Joanna Kapusta-Duch; Aneta Agnieszka Koronowicz; Sylwester Smoleń; Łukasz Skoczylas; Iwona Ledwożyw-Smoleń; Roksana Rakoczy; Edyta Maślak

    2016-01-01

    Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues a...

  7. Effects of pollution on lead and cadmium concentration and correlation with biochemical parameters in blood of human population nearby Kosovo thermo power plants

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    L. Zeneli

    2008-01-01

    Full Text Available This study describes an investigation of lead and cadmium pollution of Kosovo environment as a result of outflow from the coal processing industry. In a comparative study of lead and cadmium concentration in blood of human population of two different environments in Kosovo, one nearby Kosovo Thermo Power Plants, (Obiliq a highly polluted environment and the other that is considered as relatively clean rural environment (Dragash. Analysis has shown that emission of particulate in fly ash from Thermo Power Plants during 2005 has exceeded EU standards by 400-500% and that lead concentration was 18mg kg-1 and cadmium concentration was -1 of ash. A series of determinations of lead and cadmium concentrations in blood of population that lives in this environment, have shown direct effects in biochemical parameters CRE (Creatinin, DB (Direct Bilirubine, TB (Total Bilirubine, AST (Aspartat Aminotransferaza, CK (Creatin Kinaza and CHE (Cholenisteraza in human organism. The results that were achieved in this study showed a significant difference in average lead and cadmium concentration in the blood of the investigated group of peoples that lives in the area near by the Power Plants, from a control group that lives in a rural unpolluted environment. Lead and cadmium has been analyzed in 50 samples taken from persons from industrial zone and 25 samples in controlled group. The level of lead concentration was 23.0-112.1 µg L-1in geometric average 46.05 µg L-1, cadmium concentration was 0.44-6.02 µg L-1 in geometric average of 1.56µg L-1. Controlled group from the rural relatively clean environment showed lead concentration of 6.7-33.8 µg L-1 in geometric avarage 17.76 µg L-1 and cadmium concentration of 0.21-1.8 µg L-1 or in geometric average of 0.73 µg L-1. In conclusion in exposed subjects, pollution from coal burning in Power Plant is very important factor for level of lead and cadmium concentration in blood of tested population.

  8. Effects of sublethal concentrations of tobacco (Nicotiana tobaccum leaf dust on some biochemical parameters of Hybrid catfish (Clarias gariepinus and Heterobranchus bidorsalis

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    Kabir Mohammed Adamu

    2011-02-01

    Full Text Available The aim of this work was to study the effect of tobacco (Nicotiana tobaccum leaf dust at sublethal concentrations (375.6, 187.8, 93.9 0.0mg/L on biochemical parameters of Hybrid catfish in a static semi-renewable bioassay system with the aim to ascertain its effect on the test fish after 14 days exposure period. The effects of the plant dust on the test fish was dose-dependent, revealing significant difference in albumin, glucose, triglyceride, cholesterol, urea, and uric acid of the serum, liver and kidney; total protein and globulin of serum and kidney and creatinine and calcium of the serum while insignificant difference were observed for calcium and creatinine in liver and kidney and total protein and globulin in liver compared to the control. The effect of the plant dust was most pronounced in the serum, less in the kidney and least in the liver. However, the monitored water quality parameters revealed that the plant dust had effects on primary productivity, thus the biodiversity of organisms.

  9. Diazinon toxicity affects histophysiological and biochemical parameters in rabbits.

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    Yehia, Mona A H; El-Banna, Sabah G; Okab, Aly B

    2007-11-01

    Diazinon is a widely used pesticide in agriculture. So, the current work aimed to investigate the effects of diazinon exposure on some physiological and biochemical parameters, as well as, histopathological changes and histochemical acetyl-cholinesterase activity (AChE). The red Baladi rabbits were dipped into water (Control Group), diazinon at low concentrations of 0.6 mg diazinon low concentration (DLC) or high concentration of 3mg diazinon high concentration (DHC) dissolved in 1l of water for 10s. Treatment was repeated after 10 days and animals were sacrificed between 0 and 21 days after the second treatment. Blood analysis revealed that Red blood cells (RBC's), hemoglobin (Hb) and plasma total protein (TP) were significantly decreased in both diazinon concentrations (Pliver/ body weight and cytochrome P-450 were decreased in both concentrations (Pliver, kidney and brain were observed after DHC dipping. Glycogen content was decreased in liver and increased in kidney Bowman's capsule. Furthermore, AChE activity was inhibited in brain tissue, decreased in liver cells, but gradually increased in kidney glomerular cells. Therefore, kidney and brain were highly affected by diazinon exposure compared with the liver. Exposure of animals to diazinon caused extensive changes in physiological, biochemical, and histopathological parameters as well as histochemical AChE. So, contact exposure of diazinon leads to negative response on animal health.

  10. Effects of iron-glycine chelate on growth, carcass characteristic, liver mineral concentrations and haematological and biochemical blood parameters in broilers.

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    Kwiecień, M; Samolińska, W; Bujanowicz-Haraś, B

    2015-12-01

    Studies were carried out to determine the effect of additive iron-glycine chelate on the production performance, slaughter yield, mineral deposition in the liver and the metabolic blood panel in broiler chickens. A total of 250 one-day-old Ross 308 chicks were allotted into five groups with five replicates of 10 birds each. Diets were supplemented with the organic form iron (Fe-Gly at the rate of 25%, 50% or 100% of the total requirements of the elements) and inorganic Fe (FeSO4 at the rate of 50% or 100%). In the experiment, iron was added to the premix (containing no Fe) in an amount of 40 or 20 mg per kg of basal diet, in groups I and II, in the form of FeSO4 , and in an amount of 40, 20 or 10 mg per kg of basal diet, in groups III, IV and V, in the form of Fe-Gly. The study covering the period from the first to the 42nd day of breeding revealed that the production performance and slaughter yield were not dependent on the form and amount of added Fe. In the experimental groups with the addition of Fe-Gly of 20 or 10 mg/kg, there were no deaths of chickens during the whole fattening period. As a result, introducing an organic form of iron covering 50% and 25% of the birds' requirement increased the effectiveness of chicken fattening (European Efficiency Index) (p < 0.01). An organic Fe compound (40, 20 or 10 mg/kg) added to mixtures contributed to significant changes in the level of biochemical and haematological indicators in blood. The study demonstrated that an addition of Fe-Gly to mixtures for broilers can be fully effective in terms of production and health performance even if the suggested requirement for this element is 50% or 25% covered.

  11. Effect of the incorporation of date pits and orange pulp in rations composed of wheat straw and concentrate on the blood biochemical parameters of Ouled Djellal breeding

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    N. Lakhdara

    2014-12-01

    Full Text Available Twenty four lambs of Ouled Djellal breeding from the region of Constantine, Algeria, were assigned randomly into 4 groups, the mean initial weights within the groups, ranged between 37.6±4.27 and 39.8±5.41 kg, to investigate the effect of the incorporation of two by-products of food industry, fresh orange pulp, ground date pits in rations composed of wheat straw as roughage, and concentrate as supplement. Four feeding groups were formed, the first group (T1 was fed with wheat straw and concentrate (60%/40%, the second group (T2 with wheat straw and orange pulp (60%/40%, for group 3 and 4 (T3-T4, the diet consisted on a mixture of 60% wheat straw and date pits at a ratio of (80 to 20% as a roughage in addition to 40% orange pulp for T3 and 40% concentrate for T4. Blood samples were collected from the jugular vein before morning feeding. Values of animal's plasma levels of Ca, glucose, proteins and urea were measured using a UV spectrophotometer. There was no significant difference in all the diets for Ca value, Ca values varied between 8.37 and 10.74 mg/Dl. T4 showed the highest value. Glucose blood content was similar for all the animals with no significant differences. While a very significant difference <0.001 was observed in blood proteins level in T3 and T4 comparing to the other groups. When date pits were incorporated in the diet containing wheat straw and concentrate, a very significant difference on urea blood content of lambs was observed (P<0.001.

  12. The effects of different levels of Chlorella microalgae on blood biochemical parameters and trace mineral concentrations of laying hens reared under heat stress condition

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    Moradi kor, Nasroallah; Akbari, Mohsen; Olfati, Ali

    2016-05-01

    This study was conducted to investigate the effect of different supplementation levels of Chlorella microalgae on serum metabolites and the plasma content of minerals in laying hens reared under heat stress condition (27.5-36.7 °C, variable). A total number of 378 (40 weeks of age, with mean body weight of 1390 ± 120 g) were randomly allocated to six treatments with seven replicates. The birds were randomly assigned to 6 treatments (C, T1, T2, T3, T4, and T5) with 7 replicate cages of 9 birds. C. microalgae at the rates of 100, 200, 300, 400, and 500 ppm with water were offered to groups T1, T2, T3, T4, and T5, respectively, while group C served as a control. At 71 days of trial, blood samples (14 samples per treatment) were taken for measuring serum metabolites and at 72 days for plasma mineral analysis. The results of this experiment showed that the supplementation of 200-500 ppm C. microalgae decreased the serum content of cholesterol, triglycerides, and LDL ( P HDL content increased ( P < 0.05) in the hens supplemented with C. microalgae (300 or 400 and 500 ppm). C. microalgae at rates of 300-500 ppm caused a marked ( P < 0.05) increase in the plasma content of manganese or iodine and selenium but other minerals were not statistically different among treatments. Overall, from the results of the present experiment, it can be concluded that supplementation of C. microalgae at high rates was beneficial on blood parameters of laying hens reared under heat stress.

  13. Serum biochemical parameters of farmed carp (Cyprinus carpio

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    Tanţi Patriche

    2011-04-01

    Full Text Available Despite advances in ichthyo-pathology of recent years, interpretation of fish serum biochemical parameters is often difficult by lack of reference values. That is why to know the value of the serum biochemical parameters can be a useful tool for monitoring health status, detecting illnesses and responses to therapy. This paper provides data concerning biochemical composition of carp serum (Cyprinus carpio bred at Brateş Farm of Institute of Research and Development for Aquatic Ecology, Fishing and Aquaculture from Galaţi and Pleaşa Farm from Ploieşti, Romania. In research conducted onCyprinus carpio were determined following serum biochemical parameters: glucose (GLU, total proteins (TP, blood urea nitrogen (BUN, cholesterol (CHOL, triglyceride (TRIG, sodium (Na, potassium (K, calcium (Ca, magnesium (Mg, phosphorus (P, iron (Fe.

  14. Accelerated maximum likelihood parameter estimation for stochastic biochemical systems

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    Daigle Bernie J

    2012-05-01

    Full Text Available Abstract Background A prerequisite for the mechanistic simulation of a biochemical system is detailed knowledge of its kinetic parameters. Despite recent experimental advances, the estimation of unknown parameter values from observed data is still a bottleneck for obtaining accurate simulation results. Many methods exist for parameter estimation in deterministic biochemical systems; methods for discrete stochastic systems are less well developed. Given the probabilistic nature of stochastic biochemical models, a natural approach is to choose parameter values that maximize the probability of the observed data with respect to the unknown parameters, a.k.a. the maximum likelihood parameter estimates (MLEs. MLE computation for all but the simplest models requires the simulation of many system trajectories that are consistent with experimental data. For models with unknown parameters, this presents a computational challenge, as the generation of consistent trajectories can be an extremely rare occurrence. Results We have developed Monte Carlo Expectation-Maximization with Modified Cross-Entropy Method (MCEM2: an accelerated method for calculating MLEs that combines advances in rare event simulation with a computationally efficient version of the Monte Carlo expectation-maximization (MCEM algorithm. Our method requires no prior knowledge regarding parameter values, and it automatically provides a multivariate parameter uncertainty estimate. We applied the method to five stochastic systems of increasing complexity, progressing from an analytically tractable pure-birth model to a computationally demanding model of yeast-polarization. Our results demonstrate that MCEM2 substantially accelerates MLE computation on all tested models when compared to a stand-alone version of MCEM. Additionally, we show how our method identifies parameter values for certain classes of models more accurately than two recently proposed computationally efficient methods

  15. Biochemical Parameters of Orienteers Competing in a Long Distance Race.

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    Mikan, Vladimir; And Others

    1992-01-01

    Measured important biochemical parameters in a group of orienteers two hours before beginning and immediately after an orienteering marathon. Found levels of dehydration. Suggests a drinking regimen which is designed for orienteering races. Concludes that no runner having kidney or liver abnormalities or changes in the urine should be allowed to…

  16. Assessment of biochemical concentrations of vegetation using remote sensing technology

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The main biochemicals (such as lignin, protein, cellulose, sugar, starch, chlorophyll and water) of vegetation are directly or indirectly involved in major ecological processes, such as the functions of terrestrial ecosystems (i.e., nutrient-cycling processes, primary production, and decomposition). Remote sensing techniques provide a very convenient way of data acquisition capable of covering a large area several times during one season, so it can play a unique and essential role provided that we can relate remote sensing measurements to the biochemical characteristics of the Earth surface in a reliable and operational way. The application of remote sensing techniques for the estimation of canopy biochemicals was reviewed. Three methods of estimating biochemical concentrations of vegetation were included in this paper: index, stepwise multiple linear regression, and stepwise multiple linear regression based on a model of the forest crown. In addition, the vitality and potential applying value are stressed.

  17. The in vitro effect of drugs on biochemical parameters determined by a SMAC system.

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    Vinet, B; Letellier, G

    1977-02-01

    1. We have studied the in vitro effect of 39 drugs on 17 biochemical parameters determined by a SMAC System. Only two drugs were found to interfere: ascorbic and theophyline. 2. The ascorbic acid lowers the glucose and the bilirubine values; it increases the creatinine and the uric acid concentration. At concentration smaller than 5 mg/dl of this drug, these effects are negligible. 3. We have found a new drug interference: theophylline inhibits the alkaline phosphatase and LDH activities. This effect is not negligible on alkaline phosphatase for therapeutic levels of this drug; the action on LDH can be ignored at normal therapeutic range. 4. For a given drug, we have found different interference with biochemical parameters determined with various commercial lyophlised control sera or a liquid pool of sera. This indicates that the type of sera used in drug interference studies must be described.

  18. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan

    Institute of Scientific and Technical Information of China (English)

    Shumaila Kiran; Arif Mahmood Bhutta; Bakhtyawar Ali Khan; Sobia Durrani; Muhammad Ali; Muhammad Ali; Furhan Iqbal

    2012-01-01

    Objective: To report and compare the hematological and serum biochemical profile of goat and sheep from Rahim Yar Khan district in Southern Punjab. Methods: One hundred and twenty blood samples (98 goat and 22 sheep) were collected and their blood glucose, hemoglobin and serum biochemical parameters, cholesterol, aspartate transaminase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were determined by using spectrophotometer. Parameters were compared between goat and sheep. Results: It was observed that glucose (P=0.001), LDH (P=0.001) and ALT (P=0.001) concentrations differed significantly between sheep and goats. The studied parameters were compared between buck and goats and also between young goats (less than 12 months old) and adults (more than 12 months old). It was found that age and gender did not affect these hematological and serum biochemical parameters.Conclusions: It can be concluded that sheep have overall higher values than goats for studied hematological and serum biochemical parameters.

  19. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

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    Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

  20. Dynamics of biochemical parameters of blood serum in kidney injuries

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    Y. L. Podgainiy

    2014-12-01

    Full Text Available Aim. Annually injuries of varying severity are registered in more than 4,5 million people (up to 10% of the population in Ukraine; renal injury in polytrauma is detected in 26,4% of cases and takes 2 – 3 place of injury of the abdominal cavity and retroperitoneal space. In order to study the kidney function and other vital organs systems 108 patients were examined. Methods and results. Laboratory methods (clinical and biochemical parameters of blood and urine tests, ultrasound and CT scans of the kidneys and abdominal organs were used. Conclusion. It was established that polytrauma often occurs in males (73,5% of middle-age. 42% of patients presented renal function violation - nitrogen excretion and 84% of patients had activated blood coagulation in the first 7 – 10 days of injury.

  1. Correlations between endogen amylin hormone and some hormonal, biochemical and bone parameters in pullets

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    S Guzel

    2014-12-01

    Full Text Available The objective of this study was to assess the correlations of amylin (a pancreatic polypeptide hormone with some hormonal, biochemical and bone parameters in pullets. Forty 18-week-old pullets were used. Plasma amylin, CT (calcitonin, 1,25 (OH2 vitamin D (1,25 dihydroxycholecalciferol , serum osteocalcin, glucose, ALP (alkaline phosphatase, cholesterol, and triglycerides, as well as weight, length and total volume of tibiotarsi were measured. Plasma amylin concentration was negatively correlated with serum cholesterol (p<0.05 and triglycerides (p<0.05 concentrations. Plasma amylin concentration was significantly and positively correlated with plasma calcitonin concentrations (p<0.001. Serum ALP and plasma amylin concentrations were positively correlated (p<0.01. There were no correlations between amylin hormone and other parameters. Based on these results, it is possible to conclude that endogen amylin may effect cholesterol, triglycerides, calcitonin, and ALP levels in pullets without changing some other hormonal, biochemical and bone parameters related to calcium and lipid metabolism.

  2. Hematological parameters in relation to age, sex and biochemical values for mute swans (Cygnus olor).

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    Dolka, B; Włodarczyk, R; Zbikowski, A; Dolka, I; Szeleszczuk, P; Kluciński, W

    2014-06-01

    The knowledge of the correct morphological and biochemical parameters in mute swans is an important indicator of their health status, body condition, adaptation to habitat and useful diagnostic tools in veterinary practice and ecological research. The aim of the study was to obtain hematological parameters in relation to age, sex and serum biochemistry values in wild-living mute swans. We found the significant differences in the erythrocyte count, hematocrit, hemoglobin concentration and erythrocyte sedimentation rate in relation to age of mute swans. There were no differences in hematological values between males and females. The leukogram and H/L ratio did not vary by age and sex in swans. Among of biochemical parameters the slightly increased AST, ALP, CK, K, urea, decreased CHOL and TG values were recorded. As far as we know, this is the first study in which the morphometric parameters of blood cells in mute swans were presented. We found extremely low concentration of lead in blood (at subthreshold level). No blood parasites were found in blood smears. The analysis of body mass and biometric parameters revealed a significant differences dependent on age and sex. No differences in the scaled mass index were found. Our results represent a normal hematologic and blood chemistry values and age-sex related changes, as reference values for the mute swan.

  3. Effects of intermittent lighting on broiler growth performance, slaughter performance, serum biochemical parameters and tibia parameters

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    Haiming Yang

    2015-12-01

    Full Text Available The objective of this study was to investigate the effects of intermittent lighting (IL on broiler growth and slaughter performance as well as serum biochemical and tibia parameters. Two hundred and eighty 7-d-old Ross 308 broilers were selected and reared from 8 to 42 d under IL photoperiods of either 2 hours of light:2 hours of dark (2L:2D or 4 hours of light:4 hours of dark (4L:4D, or under continuous lighting (CL. On day 42, body weight of broilers raised in the 4L:4D photoperiod was significantly increased compared to those reared in the 2L:2D photoperiod. The average daily feed intake (ADFI of broilers in IL was significantly less than broilers in CL. The feed conversion of broilers raised in the 4L:4D photoperiod was significantly more efficient than broilers in CL. The intestinal tract weight was greater in birds raised in the 4L:4D photoperiod compared to birds in CL. Similarly, the eviscerated yield with giblet and eviscerated carcass weights in birds reared in the 4L:4D photoperiod were significantly higher than corresponding weights observed in broilers raised in CL. Serum total protein concentration was distinctly higher in birds raised in the 4L:4D photoperiod than that in the 2L:2D photoperiod. In addition, the bone elastic modulus of birds reared in the 4L:4D photoperiod was greater than measurements observed in the 2L:2D photoperiod. Accordingly, we concluded that the 4L:4D photoperiodic lighting schedule was superior to both the 2L:2D photoperiod and CL in broiler production.

  4. Evaluation of clinical, biochemical and hematological parameters in macrocytic anemia

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    Aarthi Kannan

    2016-07-01

    Results: Primary bone marrow disorders were the most common cause of macrocytosis (46%. The other causes in decreasing order of frequency were megaloblastic anaemia (38%, hemolytic anemia (6%, drug induced (5%, alcoholism and liver disease (4% and idiopathic thrombocytopenic purpura (1%. There was a significant difference in the mean values of MCV and serum LDH between megaloblastic and non and ndash; megaloblastic macrocytosis. When serum LDH >1345.2 IU/L or MCV>121fl (criterion values of ROC curve with reticulocyte count <2% was taken as criteria, the sensitivity was 92.1% and specificity was 93.5% for diagnosing megaloblastic anemia. Conclusions: Systematic evaluation of macrocytosis will help us to distinguish megaloblastic and non and ndash; megaloblastic macrocytosis. The blood and biochemical parameters especially CBC, RC, and serum LDH along with supporting clinical features help us in diagnosing megaloblastic anemia in a setup where vitamin and metabolite levels are difficult to obtain. [Int J Res Med Sci 2016; 4(7.000: 2670-2678

  5. Clinical and Biochemical Parameters of Children and Adolescents Applying Pesticides

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    O Hendy

    2010-06-01

    Full Text Available Background: The primary agricultural product in Egypt is the cotton crop. Children and adolescents work seasonally in the cotton fields applying pesticides.Objectives: To examine the effect of pesticide exposure on clinical and biochemical parameters in children and adolescents applying pesticides.Methods: Male children currently applying pesticides and aged between 9 and 19 years (n = 50 were recruited for this study. They were asked to complete work, health, and exposure questionnaires; examined for any medical and neurological problems with particular attention to sensory and motor functions including cranial nerves, sensory and motor system, and reflexes. From each participant, a blood sample was taken to measure acetylcholinesterase activity, and liver and kidney functions. Children who have never worked in agriculture (n = 50, matched on age, education, and socioeconomic status were also studied and served as controls.Results: More neuromuscular disorders were identified in pesticide applicators than controls. A significant lower level of acetylcholinesterase was found in the applicator group compared to the controls. There was also a significant difference in hematological, renal and hepatic indices in the exposed children compared to the control children. Working more days in the current season and also working more years as a pesticide applicator were both associated with an increase in the prevalence of neuromuscular abnormalities and significant changes in the laboratory tests.Conclusion: Children and adolescent pesticide applicators working in farms of Egypt are at risk of developing serious health problems similar to those of adults.

  6. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

    Directory of Open Access Journals (Sweden)

    Petra Hrabčáková

    2014-01-01

    Full Text Available The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P<0.05 in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P<0.05. At the end of the laying period, an increase (P<0.05 was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P<0.05 were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.

  7. Evaluation of nutritional and biochemical parameters in spontaneously hypertensive rats following antihypertensive treatment

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    Joanna Suliburska

    2014-03-01

    Full Text Available Introduction. One side effect of antihypertensive drugs is their impact on nutritional status and metabolism. The purpose of this study was to assess the nutritional and biochemical parameters in spontaneously hypertensive rats following treatment with antihypertensive drugs. Material and methods. The experiment was performed on 50 male spontaneously hypertensive rats (SHR, which were assigned to fi ve groups: control (C, with perindopril (PR, with metoprolol (MT, with indapamide (ID, and with amlodipine (AM. All rats were provided ad libitum standard diet (with or without drugs and distilled water. After 45 days, the animals were weighed and killed. Liver, kidney, heart, spleen, pancreas, and blood samples were collected. Concentrations of glucose, cholesterol, triglycerides, and albumin were assayed in serum. Morphology parameters, such as white blood cell, red blood cell, hematocrit, and lymphocyte counts were measured in the blood. Blood pressure was measured using a tail-cuff plethysmograph. Results. The results obtained indicate that the hypotensive drugs under investigation had no effect on the selected nutritional parameters. Perindopril signifi cantly decreased the relative mass of the heart and amlodipine markedly decreased the relative mass of the pancreas. A markedly higher concentration of glucose in the group with indapamid, and a signifi cantly lower concentration of triglycerides in the group with metoprolol, were observed. Indapamide and amlodipine markedly increased the value of red blood cells and hematocrit in the blood of SHR. Conclusions. Long-term therapy with antihypertension drugs may infl uence tissue mass and biochemical and morphological status in the body.

  8. [Design of high-efficiency double compound parabolic concentrator system in near infrared noninvasive biochemical analysis].

    Science.gov (United States)

    Gao, Jing; Lu, Qi-Peng; Peng, Zhong-Qi; Ding, Hai-Quan; Gao, Hong-Zhi

    2013-05-01

    High signal-to-noise ratio (SNR) of system is necessary to obtain accurate blood components in near infrared noninvasive biochemical analysis. In order to improve SNR of analytical system, high-efficiency double compound parabolic concentrator (DCPC) system was researched, which was aimed at increasing light utilization efficiency. Firstly, with the request of collection efficiency in near infrared noninvasive biochemical analysis, the characteristic of emergent rays through compound parabolic concentrator (CPC) was analyzed. Then the maximum focusing angle range of the first stage CPC was determined. Secondly, the light utilization efficiency of truncated type was compared with standard DCPC, thus the best structure parameters of DCPC system were optimized. Lastly, combined with optical parameters of skin tissue, calculations were operated when incident wavelength is 1 000 nm. The light utilization efficiency of DCPC system, CPC-focusing mirror system, and non-optical collecting system was calculated. The results show that the light utilization efficiency of the three optical systems is 1.46%, 0.84% and 0.26% respectively. So DCPC system enhances collecting ability for human diffuse reflection light, and helps improve SNR of noninvasive biochemical analysis system and overall analysis accuracy effectively.

  9. Biochemical lesions of platelets stored as concentrates in PVC bags.

    Science.gov (United States)

    Krishnan, L K; Mathai, J; Sulochana, P V; Jacob, J; Sivakumar, R

    1997-02-01

    Acid-base status of platelet suspension during storage is a measure of the gas permeability of the bag material. To assess the efficacy of the bags available in our market to store platelets, we compared biochemical lesions of platelets stored in an Indian polyvinyl chloride (PVC) triple bag against a Japanese PVC bag standardized for 5 days platelet storage. Platelet concentrates prepared in both control and test PVC bags were stored for 72 h. Two ml samples were drawn 1 h after preparation, and then at 24 h intervals, for analysis. Our data show that the mean pH value in the test bags was maintained above 6.5. However, the CO2 tension was high and O2 tension was low. We also analyzed malondialdehyde (MDA) formation which is a measure of arachidonic acid metabolism, and seemed to be unaffected in stored platelets. Lactate dehydrogenase (LDH) was not released into the plasma excessively and hence significant platelet lysis was absent during storage. Hypotonic shock response (HSR) of platelets stored in both test and control bags was comparable, indicating the possibility of satisfactory post-transfusion recovery.

  10. A software for the estimation of binding parameters of biochemical equilibria based on statistical probability model.

    Science.gov (United States)

    Fisicaro, E; Braibanti, A; Sambasiva Rao, R; Compari, C; Ghiozzi, A; Nageswara Rao, G

    1998-04-01

    An algorithm is proposed for the estimation of binding parameters for the interaction of biologically important macromolecules with smaller ones from electrometric titration data. The mathematical model is based on the representation of equilibria in terms of probability concepts of statistical molecular thermodynamics. The refinement of equilibrium concentrations of the components and estimation of binding parameters (log site constant and cooperativity factor) is performed using singular value decomposition, a chemometric technique which overcomes the general obstacles due to near singularity. The present software is validated with a number of biochemical systems of varying number of sites and cooperativity factors. The effect of random errors of realistic magnitude in experimental data is studied using the simulated primary data for some typical systems. The safe area within which approximate binding parameters ensure convergence has been reported for the non-self starting optimization algorithms.

  11. Rhythmic 24-hour variations of frequently used clinical biochemical parameters in healthy young males - The Bispebjerg study of diurnal variations

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Henrik L; Gøtze, Jens Peter;

    2012-01-01

    Purpose. To evaluate the influence of time of day on the circulating concentrations of 14 frequently used clinical biochemical parameters in the Bispebjerg study of diurnal variations. Materials and methods. Venous blood samples were obtained under controlled environmental, activities and food...

  12. Sperm quality and selected biochemical parameters of seminal fluid in dogs with benign prostatic hyperplasia.

    Science.gov (United States)

    Krakowski, L; Wąchocka, A; Brodzki, P; Wrona, Z; Piech, T; Wawron, W; Chałabis-Mazurek, A

    2015-09-01

    Benign prostatic hyperplasia (BPH) in dogs is most commonly associated with age and increasing concentrations of dihydrotesterone, a hormone that stimulates growth and secretion of the prostatic epithelial cells. During this process, the biochemical composition of prostatic secretion changes, which can affect the quality of semen and limit the ability of the sperm to contribute to fertilization. Therefore, the present study was conducted to examine possible correlation between BPH and biological quality of semen. The study was performed in 11 sexually mature dogs of various breeds. Animals were divided into two groups: healthy dogs (Group I; n = 5; mean age 4.32; SEM = 1.28) and dogs with BPH (Group II n = 6; mean age 6.16; SEM = 0.65). Semen and prostate secretions were collected and evaluated in this study. Standard semen examinations were conducted in the ejaculates collected; moreover, the extent of apoptosis and DNA defragmentation was determined. The selected biochemical parameters were determined in the prostate secretion. According to the examination results, there were no significant differences in standard semen parameters between the two groups of dogs. Nevertheless, morphological tests of semen in dogs with BPH demonstrated elevated percentages of primary defects in spermatozoa. A significant increase (P = 0.01) in DNA defragmentation of sperm was found in dogs with BPH. Moreover, changes in the biochemical composition of prostate secretion were demonstrated. In dogs with BPH, pH of prostate secretions was greater (P = 0.03), concentrations of cholesterol increased while concentrations of Zn and Cu decreased. The study findings reveal that BPH does not change semen quality in dogs.

  13. Effect of methanol extract of Synsepalum dulcifium pulp on some biochemical parameters in albino rats

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    Nkwocha Chinelo

    2015-03-01

    Full Text Available Objective: To determine the beneficial effects of the methanol extract of Synsepalum dulcificum on some biochemical parameters. Methods: In this study, rats were orally administered (gavage with methanol extracts at doses of 0 mg/kg (Group 1, as normal group, 100 mg/kg (Group 2, 200 mg/kg (Group 3 and 500 mg/kg (Group 4 body weight per day for 28 d. Results: Acute toxicity study showed that the methanol extract was not toxic to rats up to 5 000 mg/kg. From the results, the 100 mg/kg doses of the extract significantly (P<0.05 reduced serum levels of bilirubin, low density lipoprotein, alanine aminotransferase and glucose after 14 d compared with those after 28 d. A significant difference (P<0.05 was observed in the malondialdehyde and serum protein concentration in Group 4 while glucose concentration decreased significantly (P<0.05 in Group 1 and Group 4 after 14 d compared with 28 d. The high density lipoprotein significantly increased (P<0.05 in Group 3. Conclusions: The fruit has no negative effect on some biochemical parameters in albino rats.

  14. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

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    Prokić Marko D.

    2015-01-01

    Full Text Available Aspartame (ASP is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH, as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO and lipid peroxides (LPO in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  15. Blood biochemical parameters in male American mink (Neovison vison before and during the breeding season

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    Bogdan Lasota

    2014-05-01

    Full Text Available This study was aimed at selected blood biochemical parameters in the male American mink before (September-November and during the breeding (January-March season. Blood from 143 Black and Sapphire male mink at one year age was collected. The plasma was assayed for the concentration of glucose, total protein, cholesterol, HDL and LDL fractions, triacylglycerides (TG, and the activity of ALT and AST. Concentrations of glucose, protein, total and HDL/LDL cholesterol, and AST activity were generally slightly higher during the breeding season than during the non-breeding season, but remained within the reference range. In the case of ALT activity and TG concentration, the relations were reversed. The parameters studied in the Sapphire mink showed greater variation, both in- and out of the season. In Black and Sapphire males of the American mink, the studied parameters revealed slightly higher values during the breeding season than the non-breeding season. This will vary depending on the color variety. The decrease in TG concentration during the breeding season may indicate an increased energy demand due to ambient temperature falls, and/or may be a sign of increased energy consumption associated with physical exertion during mating. Consideration should be given to the nutrition of male mink during the breeding season.

  16. The effect of intramuscular injection of spiramycin at therapeutic dose on some blood biochemical and hematological parameters in Assaf sheep

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    Ibrahim M. ALZuheir

    2012-12-01

    Full Text Available Spiramycin is used to treatment of different bacterial and protozoal infection in different animal species including sheep, to the authors’ knowledge there are no studies about effects of spiramycin in sheep blood biochemical and hematological parameters. This study was designated to determine the effects of spiramycin intramuscular treatment at therapeutic dose (64,000 IU/kg for five days in some blood biochemical and hematological parameters in healthy Assaf sheep (n=8. The results showed that spiramycin treatment caused decrease in calcium and creatinine level (PP>0.05. After treatment, hematological parameters tend to decrease toward normal references range of red blood cell count, haemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration and red blood cell distribution width (PP>0.05. All the measured biochemical and hematological parameters were in the normal references range after the treatment. These results suggested that spiramycin given in therapeutic regimen to healthy Assaf sheep caused only minor inconclusive changes in the measured hematological and biochemical profiles; and thus can be used safely in treating susceptible infections in sheep. These results might be accepted as a starting point for future experiments to evaluate the effects spiramycin on the different systems and parameters.  

  17. Haematological and biochemical blood parameters in horses used for sport and recreation

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    Katarzyna BURLIKOWSKA

    2015-12-01

    Full Text Available The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P<0.05 red blood cell count, haematocrit value and haemoglobin concentration as well as the serum level of total protein, albumin, α2- and γ-globulin, HDL and iron. The level of β- globulin, magnesium and activity of creatine kinase were significantly higher in recreationally used horses. There were no differences in the values of red blood cell parameters (MCV, MCH, MCHC, number of leucocytes and the percentage of their respective kinds, platelets, concentration of urea, creatinine, glucose, triglycerides, total cholesterol, LDL, activity of AST, ALT, LDH and in the content of Na, K, Cl, P, Ca between the tested groups. The results of the present study indicate that the type of training influences some of the resting haematological and biochemical indices in adult horses. Horses used for show jumping have higher aerobic capacity and show a more beneficial lipid profile compared to horses used for recreation.

  18. Field-effect-based multifunctional hybrid sensor module for the determination of both (bio-)chemical and physical parameters

    Science.gov (United States)

    Schoening, Michael J.; Poghossian, Arshak; Schultze, J. Walter; Lueth, Hans

    2002-02-01

    Sensor systems for multi-parameter detection in fluidics usually combine different sensors, which are designed to detect either a physical or (bio-)chemical parameter. Therefore, such systems include a more complicated fabrication technology and measuring set-up. In this work, an ISFET (ion-sensitive field-effect transistor), which is well known as a (bio-)chemical sensor, is utilized as transducer for the detection of both (bio-)chemical and physical parameters. A multifunctional hybrid module for the determination of two (bio-)chemical parameters (pH, penicillin concentration) and three physical parameters (temperature, flow velocity and flow direction) using only two sensor structures, an ion generator and a reference electrode, is realized and its performance has been investigated. Here, a multifunctionality of the sensor system is achieved by means of different sensor arrangements and/or different operation modes. A Ta2O5-gate ISFET was used as transducer for all sensors. A novel time-of-flight type ISFET-based flow-velocity (flow rate) and flow-direction sensor using in-situ electrochemical generation of chemical tracers is presented. Due to the fast response of the ISFET (usually in the millisecond range), an ISFET-based flow sensor is suitable for the measurement of the flow velocity in a wide range. With regard to practical applications, pH measurements with this ISFET were performed in rain droplets.

  19. Effects of angiotensin converting enzyme inhibitor: ramipril on different biochemical parameters in essential hypertensive patients

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    Pratibha S. Salve

    2016-06-01

    Conclusions: Ramipril has beneficial effects on RAS (Renin angiotensin system and kinin system or both may contribute to the improvement in different biochemical parameters by ramipril. [Int J Res Med Sci 2016; 4(6.000: 2288-2291

  20. Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat.

    Science.gov (United States)

    Qu, Daofeng; Zhou, Xu; Yang, Feng; Tian, Shiyi; Zhang, Xiaojun; Ma, Lin; Han, Jianzhong

    2017-06-01

    A fast, sensitive and effective method based on the blood biochemical parameters for the detection of PSE meat was developed in this study. A total of 200 pigs were slaughtered in the same slaughterhouse. Meat quality was evaluated by measuring pH, electrical conductivity and color at 45min, 2h and 24h after slaughtering in M. longissimus thoracis et lumborum (LD). Blood biochemical parameters were determined in blood samples collected during carcass bleeding. Principal component analysis (PCA) biplot showed that high levels of exsanguination Creatine Kinase, Lactate Dehydrogenase, Aspertate aminotransferase, blood glucose and lactate were associated with the PSE meat, and the five biochemical parameters were found to be good indicators of PSE meat Discriminant function analysis (DFA) was able to clearly identify PSE meat using the five biochemical parameters as input data, and the class model is an effective diagnostic tool in pigs which can be used to detect the PSE meat and reduce economic loss for the company.

  1. Relationship between daily dietary fructose intake, body composition and biochemical parameters patients with type 2 diabetes

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    Sabriye Arslan

    2016-05-01

    Conclusion: Further studies are needed to develop more specific suggestions regarding fructose intake and to better understand the relationship between fructose intakes, body composition and biochemical parameters in individuals with type 2 diabetes.

  2. Global parameter estimation methods for stochastic biochemical systems

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    Poovathingal Suresh

    2010-08-01

    Full Text Available Abstract Background The importance of stochasticity in cellular processes having low number of molecules has resulted in the development of stochastic models such as chemical master equation. As in other modelling frameworks, the accompanying rate constants are important for the end-applications like analyzing system properties (e.g. robustness or predicting the effects of genetic perturbations. Prior knowledge of kinetic constants is usually limited and the model identification routine typically includes parameter estimation from experimental data. Although the subject of parameter estimation is well-established for deterministic models, it is not yet routine for the chemical master equation. In addition, recent advances in measurement technology have made the quantification of genetic substrates possible to single molecular levels. Thus, the purpose of this work is to develop practical and effective methods for estimating kinetic model parameters in the chemical master equation and other stochastic models from single cell and cell population experimental data. Results Three parameter estimation methods are proposed based on the maximum likelihood and density function distance, including probability and cumulative density functions. Since stochastic models such as chemical master equations are typically solved using a Monte Carlo approach in which only a finite number of Monte Carlo realizations are computationally practical, specific considerations are given to account for the effect of finite sampling in the histogram binning of the state density functions. Applications to three practical case studies showed that while maximum likelihood method can effectively handle low replicate measurements, the density function distance methods, particularly the cumulative density function distance estimation, are more robust in estimating the parameters with consistently higher accuracy, even for systems showing multimodality. Conclusions The parameter

  3. Assessment of hydrodynamics, biochemical parameters and eddy diffusivity in a semi-enclosed Ionian basin

    Science.gov (United States)

    De Serio, Francesca; Mossa, Michele

    2016-11-01

    In the framework of the Italian flagship Project RITMARE, during December 2013 a meteo-oceanographic station was installed in the Mar Grande basin, a semi-enclosed bay in Southern Italy, connected to the Ionian Sea. Due to the presence of coastal heavy industry and anthropic pressure, this marine system has experienced environmental degradation over the last decades. Therefore, much monitoring of hydrodynamics and water quality indicators is required. In fact, this monitoring makes it possible to check the real-time biochemical status of the basin and therefore promptly intervene when accidental spills occur, and to create a dataset necessary to calibrate and validate modelling systems providing forecasts. The present paper aims to analyze and discuss the long term and continuous recordings of hydrodynamic and biochemical data collected by this station, available for the period from January 2014 up to December 2014. In detail, hourly measurements of wind, waves, current velocity, water temperature, salinity, chlorophyll and turbidity concentrations were archived in monthly time-series and processed in frequency domain, using the FFT (Fast Fourier Transform), to both delineate the reciprocal effects of drivers and deduce some correlations between parameters (De Serio and Mossa, 2013). Following this, monthly surface current data were processed in order to obtain time-averaged values of the turbulent velocity components, turbulent kinetic energy and turbulent time scales. Based on these calculated turbulent parameters, the horizontal eddy diffusivity was computed with the hypothesis of homogeneous turbulence using two methods, which provided results with the same order of magnitude. These results are of interest for numerical dispersion models. Finally, only referring to the month of December 2014, the time series of the crude oil concentration was available at the station and was examined in depth. The field data enabled us to conclude that the crude oil dispersion

  4. Causal correlation of foliar biochemical concentrations with AVIRIS spectra using forced entry linear regression

    Science.gov (United States)

    Dawson, Terence P.; Curran, Paul J.; Kupiec, John A.

    1995-01-01

    A major goal of airborne imaging spectrometry is to estimate the biochemical composition of vegetation canopies from reflectance spectra. Remotely-sensed estimates of foliar biochemical concentrations of forests would provide valuable indicators of ecosystem function at regional and eventually global scales. Empirical research has shown a relationship exists between the amount of radiation reflected from absorption features and the concentration of given biochemicals in leaves and canopies (Matson et al., 1994, Johnson et al., 1994). A technique commonly used to determine which wavelengths have the strongest correlation with the biochemical of interest is unguided (stepwise) multiple regression. Wavelengths are entered into a multivariate regression equation, in their order of importance, each contributing to the reduction of the variance in the measured biochemical concentration. A significant problem with the use of stepwise regression for determining the correlation between biochemical concentration and spectra is that of 'overfitting' as there are significantly more wavebands than biochemical measurements. This could result in the selection of wavebands which may be more accurately attributable to noise or canopy effects. In addition, there is a real problem of collinearity in that the individual biochemical concentrations may covary. A strong correlation between the reflectance at a given wavelength and the concentration of a biochemical of interest, therefore, may be due to the effect of another biochemical which is closely related. Furthermore, it is not always possible to account for potentially suitable waveband omissions in the stepwise selection procedure. This concern about the suitability of stepwise regression has been identified and acknowledged in a number of recent studies (Wessman et al., 1988, Curran, 1989, Curran et al., 1992, Peterson and Hubbard, 1992, Martine and Aber, 1994, Kupiec, 1994). These studies have pointed to the lack of a physical

  5. Variations of clinical biochemical parameters of laying hens and broiler chickens fed aflatoxin-containing feed.

    Science.gov (United States)

    Fernandez, A; Verde, M T; Gascon, M; Ramos, J; Gomez, J; Luco, D F; Chavez, G

    1994-03-01

    Two groups of 32 laying hens (Hyssex Brown) and two groups of 32 23-day-old (Hybro) broiler chickens were fed 2.5 and 5 parts/10(6) of aflatoxin in their diet for 4, 8, 16 and 32 days; 16 hens and 32 chicks were maintained as control groups (0 parts/10(6)). After the intoxication period, a clearance period was established of 1, 2, 4 and 8 days. Relative weights of liver and kidneys significantly increased in intoxicated hens, but not in broiler chickens. Histological lesions in both types of bird consisted of hepatic cell vacuolation with fatty infiltration. There was a significant decrease (Phens, cholesterol levels were not significantly (P> 0.05) different from control values, but triglyceride levels decreased (PAST) serum levels remained normal, whereas alanino aminotransferase (ALT) activity decreased in both intoxicated groups. The activity of serum lactic dehydrogenase (LDH) and gammaglutamil transferase (GGT) increased significantly. In intoxicated broiler chickens, aflatoxins did not alter (P> 0.05) the biochemical parameters studied, except that the serum calcium concentration was lower in the 5 parts/10(6) group. These data indicated that in intoxicated laying hens, a severe clinical biochemical alteration was produced, and that this together with the hepatic lesions observed in hens and broilers may aid disease diagnosis.

  6. Effects of ramadan fasting on cardiovascular and biochemical parameters

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    M Gupta

    2013-12-01

    Full Text Available Background: Muslims abstain from food and drink from dawn to sunset every day during Ramadan - the holiest month in Islamic lunar calendar.  Methods: The effect of Ramadan fast on body mass index (BMI, blood pressure (BP, fasting blood glucose (FBG and lipid profile were studied on 100 healthy male, adult Muslim volunteers. All parameters were recorded one week before the onset and then in the last week of Ramadan month and compared.  Results: There was no statistically significant effect on BMI, and systolic or diastolic blood pressures (p>0.05. However, fasting blood glucose (FBG, serum total cholesterol (TC, serum triglycerides (TG were significantly decreased (p = 0.000 and high density lipoprotein cholesterol (HDL-C level was significantly (p=0.000 increased in last week of Ramadan fasting compared to pre fasting levels. Conclusion: Our results show beneficial effects of Ramadan fasting on FBG and serum lipid profile. 

  7. [Changes in platelet concentrates from dogs due to storage. II. Biochemical changes in concentrate plasma].

    Science.gov (United States)

    Klein, A; Adamik, A; Mischke, R

    1999-08-01

    Platelet concentrate (PC) obtained from dogs with an automatic cell separator was stored in C4-cell separation sets with low gasdiffusionable Polyvinylchlorid (PVC) storage containers or in C4L-sets developed for storage with high gasdiffusionable Polyolefin(PO) containers, respectively. PC were stored for 10 days under permanent agitation at 22 degrees C (C4/22 degrees C, n = 10; C4L/22 degrees C, n = 11) or at 4 degrees C (C4L/4 degrees C, n = 6), respectively. Measurements were carried out directly after production of the PC, after 6 hours and then daily during the 10-day storage period. In the second part of this paper the results of pH, the concentration of bicarbonate, glucose, lactate and potassium ions as well as the activity of lactate dehydrogenase (LDH) are presented. The varying duration and intensity of the energy metabolism of the platelets and different part of glycolysis became obvious by the consumption of glucose and production of lactate, which differed significantly between the different storage conditions. Resulting from this, the mean pH decreased under the limit prescribed for human PC (pH = 6.3) already after a storage period of 3 days due to the slight capacity of gas diffusion in PVC-containers (C4/22 degrees C). In the PO-containers the pH fell below this limit at 22 degrees C (C4L/22 degrees C) after a storage period of 5 days and at 4 degrees C (C4L/4 degrees C) after 10 days. The latter reflects the high gas diffusion capacity of the PO-containers and the decreased metabolism activity at 4 degrees C. The increase of activity of LDH and of the concentration of potassium ions, which are localized in the cytosol of platelets, depended also on the different storage conditions and, thereby, reflected the different rapidity of increasing membrane permeability or the destruction of the cell membrane, respectively. The results of this study nearly are in agreement with the changes of platelet function shown in part I. Biochemical changes occur

  8. Effects of methanolic extract of Moringa oleifera leaves on semen and biochemical parameters in cryptorchid rats.

    Science.gov (United States)

    Afolabi, Ayobami Oladele; Aderoju, Hameed Adeola; Alagbonsi, Isiaka Abdullateef

    2013-01-01

    While anti-oxidant effects of Moringa oleifera in much oxidative stress related diseases have been well reported, cryptorchidism on the other hand has been shown to cause oxidative stress. However, study is scanty on the likely role of Moringa oleifera in reducing cryptorchidism-induced oxidative stress in rats has not been studied. The present study looked into the effects of methanolic extract of Moringa oleifera leaves (MEMO) on semen and biochemical parameters in cryptorchid rats. Twenty male albino rats (200-250 g) were randomly divided into 4 groups (n=5 each). Groups A and B were sham-operated and treated with corn-oil and 200 mg/kg of MEMO respectively, while groups C and D were rendered cryptorchid and also treated with corn-oil and 200 mg/kg of MEMO respectively. Cryptorchid rats had lower testicular weight, sperm count, germ cell count, testicular superoxide dismutase (SOD) concentration, testicular total protein and higher testicular malondialdehyde (MDA) concentration compared to sham-operated rats. MEMO had no significant effect on testicular weight and MDA concentration, while it significantly increased sperm count, germ cell count, testicular SOD and total protein in the cryptorchid rats. The present study suggests that MEMO ameliorates cryptorchidism associated germ cell loss and oxidative stress.

  9. Sublethal effects of an organophosphorus insecticide (RPR-II) on biochemical parameters of tilapia, Oreochromis mossambicus.

    Science.gov (United States)

    Venkateswara Rao, J

    2006-08-01

    The effect of exposure to sublethal concentrations (0.017 mg L(-1), 1/10 of LC50) of the novel organophosphate (OP) insecticide, 2-butenoic acid-3-(diethoxyphosphinothioyl) methyl ester (RPR-II) on biochemical parameters in Oreochromis mossambicus was studied during exposure for 3, 7, 15, 30 and its recovery response after seven days. Acetylcholinesterase (AChE) activity of brain, gill and muscle was inhibited by 67%, 77% and 73% respectively on day-30. The plasma and kidney alanine aminotransferase (AlaAT), and aspartate aminotransferase (AspAT) activity increased, while decreases were observed in gill and liver. Increases in acid phosphatase (AcP), and alkaline phosphatase (AP) activities were observed in plasma, gill, and kidney, and reductions of 20% and 61% in liver AcP and AP, respectively. Depletion of glycogen was observed in all tissues, an indication of typical stress related response of the fish with pesticide. Lactate dehydrogenase (LDH) activity decreased in liver and muscle, indicating tissue damage but a significant increase in LDH activity in gill and brain was observed. Depletion of glutathione (GSH) was observed in all tissues, thereby enhancing lipid peroxidation resulting in cell damage. The induction in hepatic glutathione-S-transferase (GST) levels indicates protection against the toxicity of xenobiotic-induced lipid peroxidation. There was a significant recovery in the above biochemical parameters, in all tissues of fish after a recovery period of seven days. These results revealed that the OP insecticide RPR-II is highly toxic and affects the intermediary metabolism of O. mossambicus.

  10. THE EFFECTS OF VEGETABLE LECITHIN ON SOME SANGUINE BIOCHEMICAL PARAMETERS AT LACTATING SHEEP AND GOATS

    Directory of Open Access Journals (Sweden)

    D. DRINCEANU

    2013-12-01

    Full Text Available From a complex experiment of establishing the effects of lecithin supplemented in the diet of small ruminants, in this paper we present the influence of lecithin on some sanguine biochemical parameters at lactating sheep and goat. The researches were made on a experimental group formed by 5 sheep from łurcana breed and 5 goats from Crapatina breed, which were fed in the first experimental period with a base ratio (Br formed by 1.5 kg of hill hay and 0.2 kg concentrate forage mixture, in the second experimental period the Br was supplemented with 100 g lecithin/day/animal. At the end of each experimental period were taken blood samples to establish some biochemical parameters from sanguine serum. The supplementation of base ration with 100 g lecithin/day/animal at lactating sheep and goats did not produced significant changes to values of total proteins (TS, albumin, creatinine, urea and total bilirubins (TB from sanguine serum. Contrary to expectation the cholesterol and triglycerides were higher than normal both in sheep (from 82 mg/dl to 83 mg/dl for cholesterol and from 23 mg/dl to 45 mg/dl for triglycerides but specially in goats (from 59 mg/dl to 68 mg/dl for cholesterol and from 24 mg/dl to 55 mg/dl for triglycerides. Were registered significant increment of activity of alkaline phosphatase and in γ – GT and a decrease of plasmatic activity of pancreatic α-amylase both in sheep and goats.

  11. Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna.

    Science.gov (United States)

    Bownik, Adam; Stępniewska, Zofia; Skowroński, Tadeusz

    2015-02-01

    Ectoine (ECT) is a compatible solute produced by soil, marine and freshwater bacteria in response to stressful factors. The purpose of our study was to determine the possible toxic influence of ECT on Daphnia magna. We determined the following endpoints: survival rate during exposure and recovery, swimming performance, heart rate, thoracic limb movement determined by image analysis, haemoglobin level by ELISA assay, catalase and nitric oxide species (NOx) by spectrophotometric methods. The results showed 80% survival of daphnids exposed to 50mg/L of ECT after 24h and 10% after 90h, however lower concentrations of ECT were well tolerated. A concentration-dependent reduction of swimming velocity was noted at 24 and 48h of the exposure. ECT (at 2.5 and 4mg/L) induced an increase of heart rate and thoracic limb movement (at 2.5, 4 and 20mg/L) after 24h. After 10h of the exposure to ECT daphnids showed a concentration-dependent increase of haemoglobin level synthesized and accumulated in the epipodite epithelia. After 24h we noted a concentration-dependent decrease of haemoglobin level and its lowest value was found after 48h of the exposure. ECT at a concentration of 20 and 25mg/L slightly stimulated catalase activity after 24h. NOx level was also increased after 10h of the exposure to 20 and 25mg/L of ECT reaching maximal activity after 24h. Our results suggest that ECT possesses some modulatory potential on the behaviour, physiology and biochemical parameters in daphnids.

  12. Effect of cholic acid and its keto derivatives on the analgesic action of lidocaine and associated biochemical parameters in rats.

    Science.gov (United States)

    Posa, Mihalj; Kevresan, Slavko; Mikov, Momir; Cirin-Novta, Vera; Kuhajda, Ksenija

    2007-01-01

    This study examined the effect of the structure and concentration of cholic acid and its keto derivatives on the local analgesic action of lidocaine in rats, measured by an analgesimetric method. The increase in bile acid concentrations in the administered lidocaine solution increased the duration of local anesthesia. It was found that the introduction of keto groups into the cholic acid molecule yielded derivatives with lower promotory action, i.e. decreased the duration of local anesthesia. The biochemical parameters investigated indicated that the keto derivatives of cholic acid exhibited no toxicity compared to that of cholic acid itself.

  13. Investigation of some hematological and blood biochemical parameters in cattle spontaneously infected with bovine leukosis virus

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    Sandev Nikolay

    2013-09-01

    Full Text Available The aim of the present study was to follow out the alterations in some haematological and blood biochemical parameters in cattle spontaneously infected with enzootic bovine leukosis virus with regard to the invivodifferentiation of bovine leukosis stages. The experiment included 76 cows at various ages and body weight. Serological leukosis tests were done by agar-gel immunodiffusion test with a commercial kit of Synbiotiсs (France, containing standardised gp 51 antigen and positive serum approved by the EU. On the basis of haematological results, the cows were divided into three groups: first group – EBL-seropositive with normal haemogramme; second group – EBL seropositive with altered haemogramme and third group – controls. In cows from the first and the second group, a statistically significantly increased blood cell counts was established compared to healthy controls. The total WBC were increased in the second group (leukocytosis up to 33.21×109/l vs reference range of 5-10×109/l as well as lymphocyte percentages (lymphocytosis – 81.89% (reference 40–63%. A reduction in the proportion of neutrophils to 12.78% (relative neutropenia vs the reference range of 22-49% and monocytes (monocytopenia to 1.78% (reference range 2–6% was observed. A statistically significant reduction in Ca concentrations (4.41 mg/dl and higher inorganic phosphate levels (5.28 mg/dl were established in cows from the second group. Also, ASAT activity was considerably lower – 47.03 U/l, while alkaline phosphatase increased slightly within the reference range up to 167.68 U/l and 165.81 U/l in groups one and two, respectively. The present haematological and whole blood/serum biochemical results in cows spontaneously infected with EBL virus could be used as prognostic markers of the course of the disease, to distinguish the stages of infection with regard to alive diagnostics.

  14. Effect of Different Protein Levels On Nutrient Digestion Metabolism and Serum Biochemical Parameters in Calves

    Institute of Scientific and Technical Information of China (English)

    LI Hui; DIAO Qi-yu; ZHANG Nai-feng; TU Yan; WANG Ji-feng

    2008-01-01

    The current study has been performed to examine the effects of different dietary protein levels on the growth,nutrient digestion and absorption,as well as biochemical parameters in calves.Nine healthy newborn calves were selected,randomly divided into 3 groups and fed 3 milk replacers with different protein levels(18,22,and 26%),respectively.Five period-digestion-metabolism trials were taken between 12-20,22-30,32-40,42-50,and 52-60 days after birth.All 3 groups showed a similar growth curve during 11 to 61 experimental days,however,the growth rate of the 22%crude protein(CP) group was 8.89%higher than that of the 18%CP group and 19.48%higher than that of the 26%CP group.respectively. The apparent digestibility of dry material(DM)declined gradually with age,whereas,the apparent digestibility of N,extract ether(EE)rose slightly.Compared to the 22%CP and 26%CP group,calves fed with 18%CP apparently had a lower digestibility than DM,EE,and nitrogen(N).The average apparent digestibilities of N were 69.39,75.36,and 74.55%, respectively.Both the apparent digestibility and retention of calcium(Ca)and phosphorus(P)were steady throughout the experiment,but the average apparent digestibility of P in the 26%CP group was only 63.83%,which was markedly lower than that of the 18%CP group(70.40%)and 22%CP group(69.73%).In addition,the sernm concentrations of total protein(TP),albumin(ALB),and globulin(GLOB)of the 22%CP group were higher than those in the 18%CP and 26%CP groups.The urea N(BUN)content in the 18%CP group,on the other hand,was significantly lower than that of the other two groups.The highest glucose(GLU)concentration was found in the 22%CP group(5.38 mmol L-1),at the end of the trials.The protein levels in the milk replacer affected the digestion metabolism of nutrition and the serum biochemical parameters of calves at different physiological phases.Calves fed with 22%CP milk replacer had a better growth performance than the other groups.

  15. EFFECT OF DEOXYNIVALENOL ON SOME HAEMATOLOGICAL, BIOCHEMICAL AND ANTIOXIDANT PARAMETERS OF PORCINE BLOOD IN VITRO

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    Katarína Zbyňovská

    2013-02-01

    Full Text Available The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical (cholesterol, triglycerides, total protein, urea, calcium and phosphorus and anti- and pro-oxidants parameters (superoxide dismutase - SOD, glutathione peroxidase - GPx and ROS – reactive oxygen species in porcine blood in vitro. Significantly decreased content of total protein in the group with dose of 1000 ng.l-1 DON was observed compared with the control group. In other groups (E1 with 10 ng.l-1 DON and E2 with 100 ng.l-1 slightly lower values were measures in comparison with the control group. PLT significantly decreased in the experimental group E3 when compared with E1, E2, and the control group. Concentration of GPx in porcine blood significantly (P < 0.05 decreased in E1 against the control group. Concentration of SOD significantly (P < 0.05 decreased in group E2 in comparison with E1 group. The highest value of ROS was in E2 group. Other parameters were not influenced by The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical

  16. Hematological and Biochemical Parameters in Elite Soccer Players During A Competitive Half Season

    Science.gov (United States)

    Anđelković, Marija; Baralić, Ivana; Đorđević, Brižita; Stevuljević, Jelena Kotur; Radivojević, Nenad; Dikić, Nenad; Škodrić, Sanja Radojević; Stojković, Mirjana

    2015-01-01

    Summary Background The purpose of the present study was to report and discuss the hematological and biochemical behavior of elite soccer players, in order to get more insight in the physiological characteristics of these sportsmen and to provide trainers and sports doctors with useful indicators. Methods Nineteen male soccer players volunteered to participate in this study. We followed the young elite soccer players during a competitive half season. Venous blood samples were collected between 9:00 and 10:00 a.m. after an overnight fast (10 h) at baseline, after 45 and 90 days and hematological and biochemical parameters were measured. Results Hemoglobin and hematocrit levels were significantly reduced over the observational period (psoccer training (psoccer players are characterized by significant changes in biochemical and hematological parameters over the half season, which are linked to training workload, as well as adaptation induced by the soccer training. Although the values of the measured parameters fell within the reference range, regular monitoring of the biochemical and hematological parameters is fundamental for the identification of a healthy status and related optimal performances by sport doctors and trainers and selection of a correct workload by trainers.

  17. Relationship Between Hyperspectral Parameters and Physiological and Biochemical Indexes of Flue-Cured Tobacco Leaves

    Institute of Scientific and Technical Information of China (English)

    LI Xiang-yang; LIU Guo-shun; YANG Yong-feng; ZHAO Chun-hua; YU Qi-wei; SONG Shi-xu

    2007-01-01

    The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.

  18. Effects of magnesium on erythrocyte sodium-lithium countertransport and some of plasma biochemical parameters in rabbit

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    Samad Akbarzadeh

    2009-02-01

    Full Text Available Background: Magnesium acts as an essential cofactor for the activity of many enzymes. It regulates the work of cardiovascular system. The activity of sodium–lithium countertransport (SLC and the concentrations of plasma biochemical parameters such as VLDL, LDL-cholesterol, HDL-cholesterol, sodium, potassium, urea and creatinine are changed in cardiovascular diseases. The aim of this study was to determine the effects of magnesium on SLC activity and some of the plasma biochemical parameters. Methods: New Zealand white rabbits (weighed 1350 ± 50g were chosen for these experiments. This study was conducted through two in vitro and in vivo techniques. Through in vitro method, the effects of different concentrations of magnesium on SLC activity were investigated. In order to conduct in vivo method, the rabbits were divided into two groups (5 /group. One group was treated by MgSo4 (40 mg/kg body weight through peritoneum for two weeks. For the second group, deionized water was used. The activity of SLC and mentioned biochemical parameters were determined. Results: The results of both in vitro and in vivo studies showed that magnesium can significantly decrease the SLC activity and also causes an increase in Km and decreased Vmax/Km of the system and plasma concentrations of VLDL, LDL-cholesterol, total cholesterol and triglycerides were significantly decreased. Conclusion: Magnesium may cause a reduction in blood pressure through decreasing the SLC activity and affecting the concentrations of VLDL, LDL-cholesterol, total cholesterol and triglycerides and so improvement the cardiovascular diseases.

  19. 长期饲喂高精料日粮对泌乳期山羊血液生化参数及组织中糖代谢相关基因表达的影响%Effect of long-term feeding of high concentrate diet on plasma biochemical parameters and expression of genes involved in glucose metabolism in lactating goats

    Institute of Scientific and Technical Information of China (English)

    董海波; 赵茹茜; 王绍庆; 贾媛媛; 端木永前; 陶诗煜; 倪迎冬; 张源淑; 庄苏; 沈向真

    2014-01-01

    为了研究长期饲喂高精料日粮对泌乳期山羊血液生化指标和糖代谢的影响,选择8只安装永久性瘤胃瘘管的健康经产泌乳中期山羊,分别饲喂精粗比为40∶60(low concentrate,LC,n=4)和60∶40(high concentrate, HC,n=4)的日粮。实验期共8周,实验期间采集瘤胃液,血液;实验结束时采集肝脏,肌肉,脂肪组织置于液氮速冻后于-70℃保存待测。结果显示,饲喂高精料日粮2周后山羊瘤胃液pH 显著降低,采食后瘤胃液pH 持续低于5.8超过3 h,8周后瘤胃液pH 升高维持在6.0以上,但仍低于LC组山羊。与LC组山羊相比较,HC组山羊血液中葡萄糖、游离脂肪酸含量均升高(P<0.05,P=0.071),谷草转氨酶含量有上升的趋势(0.05<P<0.1);HC组肝脏中葡萄糖转运蛋白4(Glut4)mRNA 表达有上升的趋势(P=0.06),脂肪和肌肉中丙酮酸羧化酶(PC)、线粒体型磷酸烯醇式丙酮酸羧激酶(PCK2)均呈上升趋势(P=0.066,P=0.069)。长期饲喂高精料日粮引起泌乳山羊能量代谢异常,并伴随血液代谢异常及组织糖代谢基因表达的变化。%In order to investigate the effect of feeding high concentrate diets for a long-term on plasma biochemi-cal parameters and expression of genes involved in glucose metabolism in different tissues,8 healthy multipa-rous goats in mid-lactation were rumen-cannulated in and were randomly allocated to two groups,one receiving a diet with low concentrate (LC,concentrate∶roughage,%= 40∶60)as the LC group (n= 4),and another receiving high concentrate diet (HC,concentrate∶roughage,%= 60∶40)as the HC group (n= 4).The ex-periment lasted for 8 weeks.At the end of experiment,tissues including liver,muscle and fat were sampled and immediately immersed in liquid nitrogen and then stored at -70℃for later gene expression analysis.After feeding the high-concentrate diet for 2

  20. Effect of CO2 Concentration on Growth and Biochemical Composition of Newly Isolated Indigenous Microalga Scenedesmus bajacalifornicus BBKLP-07.

    Science.gov (United States)

    Patil, Lakkanagouda; Kaliwal, Basappa

    2016-11-23

    Photosynthetic mitigation of CO2 through microalgae is gaining great importance due to its higher photosynthetic ability compared to plants, and the biomass can be commercially exploited for various applications. CO2 fixation capability of the newly isolated freshwater microalgae Scenedesmus bajacalifornicus BBKLP-07 was investigated using a 1-l photobioreactor. The cultivation was carried at varying concentration of CO2 ranging from 5 to 25%, and the temperature and light intensities were kept constant. A maximum CO2 fixation rate was observed at 15% CO2 concentration. Characteristic growth parameters such as biomass productivity, specific growth rate, and maximum biomass yield, and biochemical parameters such as carbohydrate, protein, lipid, chlorophyll, and carotenoid were determined and discussed. It was observed that the effect of CO2 concentration on growth and biochemical composition was quite significant. The maximum biomass productivity was 0.061 ± 0.0007 g/l/day, and the rate of CO2 fixation was 0.12 ± 0.002 g/l/day at 15% CO2 concentration. The carbohydrate and lipid content were maximum at 25% CO2 with 26.19 and 25.81% dry cell weight whereas protein, chlorophyll, and carotenoid contents were 32.89% dry cell weight, 25.07 μg/ml and 6.15 μg/ml respectively at 15% CO2 concentration.

  1. Changes in white blood pictures and some biochemical parameters of dairy cows in peripartum period and early lactation

    Directory of Open Access Journals (Sweden)

    Mirjana Joksimović Todorović

    2012-06-01

    Full Text Available The objective of this research was to determine the changes in the number of cells of white blood line and some biochemical parameters: concentration of glucose, concentration of non-esterified fatty acids (NEFA, activity of enzymes of aspartat aminotransferase (AST, alanin aminotransferase (ALT and gamma glutamyltranferase (GGT, in 17 Holstein-Friesian breed dairy cows in peripartum period and early lactation (on d 15 prepartum, on the day of parturition and on d 15, 30, and 45 postpartum. The highest values of total leukocytes count, neutrophiles, monocytes and basophiles count were recorded on the day of parturition, and then decreased, so that the minimum value established on d 45 of lactation. The lymphocytes count was the lowest on the day of parturation, while the eosinophils and basophiles counts were significantly different in observed time intervals. The concentration of glucose ranged from 3.07 mmol/L on d 15 prepartum to 2.71 mmol/L on d 15 postpartum. A high concentration of non-esterified fatty acids (NEFA in the postpartum period is a consequence of changes in energy balance and more intensive process of lypolisis in fat tissue, due to energy disbalance at the beginning of lactation. Established activity of enzymes AST, ALT and GGT was approximately the same in studied time intervals and was within physiological limits. The results of hematological and biochemical analysis in this paper do not indicate the development of postpartum ailments, because the investigated parameters were within physiological limits.

  2. Developmental dynamics of some blood biochemical parameters in the growing turkey (Meleagris gallopavo).

    Science.gov (United States)

    Szabó, A; Mézes, M; Horn, P; Süto, Z; Bázár, Gy; Romvári, R

    2005-01-01

    Blood serum clinical biochemical parameters of fasted BUT Big 8 male turkeys were determined at the ages of 3 days, 4, 8, 12, 16 and 20 weeks, for a follow-up of the developmental changes of some serum metabolites, enzymes and ions. The serum protein content (total protein, albumin, globulin) increased with age, indicating also the moulting-associated metabolic changes in the age interval from the 8th to the 12th weeks. Creatinine was shown to have a peak at 3 days of age (role of muscle activity in thermogenesis), while urate concentration sensitively reflected the dietary protein amount. Serum triglycerides peaked at the time of yolk catabolism, while cholesterol was shown to indicate the moulting, as was serum malondialdehyde. Serum sodium content increased throughout the study. Alanine aminotransferase and aspartate aminotransferase activities increased along the ontogeny, while alkaline phosphatase activity decreased in parallel with the growth. Serum creatine kinase activity showed an over one-magnitude increase. General metabolic and enzymatic alterations were characteristic and applicable for the description of the ontogenetic development of a precocial (post-hatch triglyceride peak), large bodied, meat-type (lactate dehydrogenase, continuously increasing creatine kinase) bird species.

  3. Parameters Obtained by Hepatobiliary Scintigraphy Have Significant Correlation with Biochemical Factors Early After Liver Transplantation

    Energy Technology Data Exchange (ETDEWEB)

    Kansoul, H.A.; Axelsson, R.; Yamamoto, S.; Savicheva, I.; Aspelin, P.; Ericzon, B.G.; Gjertsen, H. [Div. of Transplantation Surgery and Div. of Radiology, Dept. of Clinical Science, Intervention, and Technology, Karolinska Inst., Karolinska Univ. Hospital, Stockholm (Sweden)

    2007-08-15

    Background: Early postoperative hepatobiliary scintigraphy after liver transplantation is performed worldwide, but data on its significance for graft function are currently limited. Purpose: To examine the correlation between the result of early postoperative hepatobiliary scintigraphy and pre- and postoperative biochemical parameters in liver transplantation (LTx) patients. Material and Methods: Six parameters of hepatobiliary scintigraphy using {sup 99m}Tc mebrofenin were statistically analyzed in 108 LTx patients: 1) half-life of the activity of elimination of mebrofenin from the blood; 2) total clearance of mebrofenin from the blood due to all possible routes; 3) half-life of the activity due to liver uptake; 4) clearance of mebrofenin from the blood due to liver uptake; 5) time to maximal uptake in the liver; and 6) the hepatic extraction fraction (HEF) and biochemical data. Analysis between patients with preoperative normal liver function, familial amyloid polyneuropathy (FAP), and end-stage liver disease (non-FAP) was also performed. Results: Univariate and multivariate analysis revealed that total bilirubin postoperative day 3 correlated with all three scintigraphic parameters, and peak aspartate aminotransferase and alanine aminotransferase correlated with HEF. The analysis between patients with FAP and non-FAP revealed no significant difference of scintigraphic data between the two groups. Conclusion: A significant correlation between early postoperative scintigraphic results and biochemical parameters was demonstrated.

  4. Basal blood parameters of horses subjected to aerobic activity fed with lipidic concentrated

    Directory of Open Access Journals (Sweden)

    Kátia de Oliveira

    2012-02-01

    Full Text Available The feeding diets were evaluated containing low and high levels of soybean oil for horses athletes subjected to two protocols of aerobic training on the response of basal blood biochemical parameters. Four horses were used in latin square design with treatments in a 2 x 2 factorial arrangement. Treatments consisted levels of 5 and 15% oil concentrates and two aerobic training, 40' and 60' minutes. Plasmatic parameters were monitored, triglyceride (TG, total cholesterol (TC, glucose (GLU and lactate (LAC, during basal metabolism. The TG, TC, GLU and LAC from horses at rest were not affected (P> 0.05 neither of diet and physical activity, 0.21, 3.79, 4.18, 0.93 mmol L-1, respectively. It can be concluded that offer concentrate with high content of soybean oil to athletic horses in aerobic activities can be performed without altering the blood biochemical profile of basal metabolism.

  5. Meat Characteristics, Fatty Liver Weight and Blood Biochemical Parameters in Force-Feeding Geese

    OpenAIRE

    2014-01-01

    A study on growth performance, meat characteristics, fatty liver weight and serum biochemical parameters (ASAT, ALAT, alkaline phosphatase, γ-glutamyltransferase, total cholesterol, triglycerides and creatinine) in Landes geese before and after force-feeding was carried out. The overfeeding of geese with corn was taken after 90-days of age. Frequency of daily force feeding was gradually increased from 2 to 5 until the 15th day of force feeding. During force-feeding period, the body weight inc...

  6. Impact of environmental stress on biochemical parameters of bacteria reducing chromium

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    Rida Batool

    2014-06-01

    Full Text Available Chromium pollution is produced in connection with industrial processes like in tanneries. It has been suggested that bioremediation could be a good option for clean up. The stress effect of variable chromate levels, pHs and growth temperatures on biochemical parameters of two Cr(VI reducing bacterial strains Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 was investigated. Transmission electrone microscopy (TEM was performed to study the intracellular distribution of Cr(VI. It was observed that initial stress of 1000 µgmL-1 caused significant enhancement of all studied biochemical parameters at pH 7.0 and growth temperature of 37 °C showing great bioremediation potential of the strains. Transmission electron microscopy revealed that the distribution of chromium precipitates was not uniform as they were distributed in the cytoplasm as well as found associated with the periplasm and outer membrane. Fourier transform infrared spectroscopy showed the possible involvement of carboxyl, amino, sulpohonate and hydroxyl groups present on the bacterial cell surface for the binding of Cr(VI ions. Cr(VI stress brought about changes in the distridution of these functional groups. It can be concluded that the investigated bacterial strains adjust well to Cr(VI stress in terms of biochemical parameters and along that exhibited alteration in morphology.

  7. Determination of Parameters of PV Concentrating System With Heliostat

    Science.gov (United States)

    Vardanyan, R.; Norsoyan, A.; Dallakyan, V.

    2010-10-01

    The structure of PV concentrating system with heliostat is analyzed. The mathematical model of system consisting of PV concentrating module and heliostat is developed. With the use of developed mathematical model the optimal parameters of the system are determined. The results of this work can be used during the design of PV concentrating systems with heliostats.

  8. Phenozan Influence on the Physiological-Biochemical Parameters of the Young Minks Leading to Their Advanced Properties

    Directory of Open Access Journals (Sweden)

    Nikolai A. Balakirev

    2016-01-01

    Full Text Available The antioxidant influence on the physiological-biochemical parameters of productive animals is one of the most important directions in modern animal science. Phenozan is a synthetic antioxidant with high biological activities including those for animals. The positive effects of phenozan on the physiological-biochemical parameters of the mink blood were found. These effects are leading to the intensive growth, strong health, enhancement of the mink fur quality, and so forth. The total amount of the erythrocytes in the mink blood increased by about 4-5% due to phenozan inclusion in the mink diet. Even a more significant increase was found in hemoglobin content in the mink blood and erythrocytes (by about 9.5% and 8.8%, resp. due to phenozan inclusion of 100 mg/day, whereas it was found only by about 6.1–5.3% or 5.5–0.3%, owing to phenozan inclusion of 50 or 150 mg/day. For many other parameters the optimal phenozan concentration was about 100 mg/day per head according to the result obtained for all studied animal groups. Thus, phenozan can be successfully used as a biologically active compound for mink nutrition and is recommended for other farm animals.

  9. Predictive value of routine hematological and biochemical parameters on 30-day fatality in acute stroke

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    Bhatia R

    2004-04-01

    Full Text Available OBJECTIVE: This prospective study was planned to study the prognostic value of routine clinical, hematological and biochemical parameters, including platelet aggregation in patients of acute stroke, on fatality occurring during the first 30 days. MATERIAL AND METHODS: In this study 116 consecutive patients (77 males and 39 females of stroke (within 72 hours of onset were included. After clinical evaluation and neuroimaging, blood investigations, hemoglobin, total leukocyte count, platelet count, platelet aggregation, erythrocyte sedimentation rate (ESR, blood sugar, urea, creatinine, sodium, potassium, serum cholesterol, serum bilirubin, aspartate aminotransferase (SGOT, alanine aminotransferase (SGPT, albumin, and globulin estimations were performed. The patients were followed up for a maximum period of 30 days from the onset of stroke, and patients who expired were grouped as ′expired′ and the rest as ′survivors′. Logistic regression analysis was carried out among the significant parameters to identify independent predictors of 30-day fatality. RESULTS: Univariate analysis demonstrated that among hematological parameters, high total leukocyte count and ESR, at admission, correlated significantly with an undesirable outcome during the initial 30 days. Among biochemical parameters, elevated urea, creatinine, serum transaminases (SGOT and SGPT and globulin levels correlated significantly with death. Logistic regression analysis demonstrated that a low Glasgow Coma Scale (GCS score along with biochemical parameters such as high serum creatinine, SGPT, ESR and total leukocyte count correlated with death. CONCLUSION: Impaired consciousness, high total leukocyte count, raised ESR, elevated creatinine and SGPT levels, estimated within 24 hours of hospitalization, are the most important indicators of 30-day mortality in patients with first-time ischemic stroke.

  10. Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro

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    Sharuti Rathore

    2014-05-01

    Full Text Available Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in productivity. It is essential to test important medicinal plants for their salinity tolerance as research efforts aim to explore economic benefits under saline conditions. Keeping in view the importance of Stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two Stevia genotypes. Two node microcuttings were subjected to MS media supplemented with different NaCl concentrations (0, 25, 50, 75, 100, 125 mM. Chlorophyll amount was observed to be decreased as compared to sugars, proline and phenols with increased salt concentrations.

  11. Effect of dietary feed additives on haematological and serum biochemical parameters of broiler chickens.

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    Alonge EO

    2016-08-01

    Full Text Available The effect of dietary feed additives on haematological and serum biochemical parameters of broiler chickens was evaluated. 180 day-old Arbor acre broiler chicks were weighed and randomly allotted to five dietary treatments with 3 replicates of 12 birds each. Broiler starter diet (2855.7 kcal/kg ME; 23.01% and finisher diet (2911 kcal/kg; 20.71% CP were formulated. Dietary treatments were control diet (basal diet without additives, OXYT diet (basal diet with oxytetracycline at 600 ppm as antibiotic, GRO-UP diet (basal diet with probiotic at 500 ppm, MOS-500 diet (basal diet with mannan oligosaccharide at 500 ppm and MOS-1000 diet (basal diet with mannan oligosaccharide at 1000 ppm. Feed and water were supplied ad libitum. At the end of weeks 4 and 8, blood samples were collected and analyzed. The haematological and serum biochemical parameters of broiler chickens fed diets containing feed additives at the starter phase were not statistically significant (P> 0.05. At the finisher phase, there were no significant (P> 0.05 differences in all the parameters measured except in the heterophils and eosinophils where birds fed the control diets had the lowest value among all treatments. Serum globulin values were significantly (P< 0.05 different as birds fed diets containing OXYT (antibiotics recorded the lowest value among all treatments. The inclusion of prebiotics and probiotics in the diets of broiler chickens elicited no adverse effect on haematological and serum biochemical parameters, thus, they can be used as replacement for antibiotics.

  12. Effects of a low-radiotoxicity uranium salt (uranyl acetate) on biochemical and hematological parameters of the catfish, Clarias gariepinus

    Science.gov (United States)

    Al-Ghanim, Khalid A.; Ahmad, Zubair; Al-Kahem Al-Balawi, Hmoud F.; Al-Misned, Fahad; Maboob, Shahid; Suliman, El-Amin M.

    2016-01-01

    Specimens of Clarias gariepinus were treated with lethal (70, 75, 80, 85, 90, and 95 mg/L) and sub-lethal concentrations (8, 12 and 16 mg/L) of uranyl acetate, a low-radiotoxicity uranium salt. The LC 50 value was registered as 81.45 mg/L. The protein and glycogen concentrations in liver and muscles were decreased in the fish exposed to sub-lethal concentrations. The red blood cell (RBC) and white blood cell (WBC) counts, haemoglobin (Hb) concentration and haematocrit (Hct) values were decreased. Different blood indices like mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were negatively affected. Level of plasma glucose was elevated whereas protein was decreased. The level of calcium concentration (Ca) was declined in the blood of exposed fish whereas magnesium (Mg) remains unchanged. The activity level of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) was elevated in exposed fish. These effects were more pronounced in the last period of exposure and in higher concentrations. Results of the present study indicate that uranyl acetate has adverse effects on Clarias gariepinus and causes changes in the biochemical and hematological parameters of the fish.

  13. Hematological and biochemical parameters in symptomatic and asymptomatic leishmania-seropositive dogs

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    Igor Ulchar

    2015-10-01

    Full Text Available Leishmaniosis caused by Leishmania infantum is vector-born severe enzootic disease in dogs. It includes a wide spectrum of clinical symptoms, but the most characteristic are alterations in the hematopoetic system and renal failure. Also, infected animals could be asymptomatic, so the manifestation of L. infantum infection depends on many factors, including host’s immunological status. The aim of this survey was to find parameters related with hematopoetic and renal failure (hematology, biochemical parameters – urea, creatinine, serum proteins in symptomatic and asymptomatic dogs seropositive for canine leishmaniosis. Within the hematological parameters, we found signifi cant differences between symptomatic and asymptomatic dogs in the erythrogram and platelet count, but not in the leukogram. Significant differences between the two groups were found also for urea, creatinine, serum albumin and globulin, but not in serum total protein and A/G ratio. These findings indicate individual variability of the host’s response to infection with L. infantum.

  14. The Hypertrophic Marchigiana: physical and biochemical parameters for meat quality evaluation

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    F. M. Sarti

    2010-04-01

    Full Text Available The aim of this study was to evaluate the meat quality of double muscled Marchigiana young bulls characterized by different genotypes for the hypertrophy: normal and mutated (heterozygous. Calpain and calpastatin activities were determined to verify the state of aging meat on a sample of Longissimus thoracis muscle (XIII thoracic rib taken at slaughtering (0h and after 24 hours (24h. After 14 days of aging, another sample of muscle was taken to evaluate physical and chemical parameters of meat quality. The results showed a better meat quality of mutated animals respect normal animals. Another interesting result was the correlation between the biochemical parameters and some physical parameters, such as WBS (Warner Bratzler Shear Force, CL (Cooking loss. These results showed the relationship between the proteolytic activity of calpain system and meat tenderness.

  15. Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows.

    Science.gov (United States)

    Rasolomboahanginjatovo, Hasina Santatriniaina; Chorfi, Younès; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

    2014-01-01

    The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE.

  16. [Simulation of vegetation indices optimizing under retrieval of vegetation biochemical parameters based on PROSPECT + SAIL model].

    Science.gov (United States)

    Wu, Ling; Liu, Xiang-Nan; Zhou, Bo-Tian; Liu, Chuan-Hao; Li, Lu-Feng

    2012-12-01

    This study analyzed the sensitivities of three vegetation biochemical parameters [chlorophyll content (Cab), leaf water content (Cw), and leaf area index (LAI)] to the changes of canopy reflectance, with the effects of each parameter on the wavelength regions of canopy reflectance considered, and selected three vegetation indices as the optimization comparison targets of cost function. Then, the Cab, Cw, and LAI were estimated, based on the particle swarm optimization algorithm and PROSPECT + SAIL model. The results showed that retrieval efficiency with vegetation indices as the optimization comparison targets of cost function was better than that with all spectral reflectance. The correlation coefficients (R2) between the measured and estimated values of Cab, Cw, and LAI were 90.8%, 95.7%, and 99.7%, and the root mean square errors of Cab, Cw, and LAI were 4.73 microg x cm(-2), 0.001 g x cm(-2), and 0.08, respectively. It was suggested that to adopt vegetation indices as the optimization comparison targets of cost function could effectively improve the efficiency and precision of the retrieval of biochemical parameters based on PROSPECT + SAIL model.

  17. Haematological and Serum Biochemical Parameters of Local Turkey Poults Fed Diets Containing Two Varieties of Sorghum

    Directory of Open Access Journals (Sweden)

    E.B. Etuk

    2012-12-01

    Full Text Available These studies were conducted to determine the effects of two varieties of sorghum, Samsorg 17 and ICSV 400 on the haematological and serum biochemical parameters of local turkey breeds, reared in Nigeria. Two hundred and sixteen poults were divided into 9 treatment groups of 24 each, which were further replicated thrice and fed starter diets containing Samsorg 17 and ICSV 400. Similar (P > 0.05 RBC and PCV values were obtained with the two diets. Samsorg 17 fed poults produced lower, though not significantly (P > 0.05 serum albumin, glucose, urea, creatinine, sodium, chloride, ALP, SGPT and SGOT values than those on ICSV 400 diet. Higher RBC, MCHC, MCH, MCV and PCV values were observed with Samsorg 17 fed turkeys than those on ICSV 400 diets. Serum glucose and creatinine decreased and SGOT increased with dietary sorghum. Similar (p > 0.05 Hb, WBC, MCHC, MCV and PCV values were obtained in all groups. Values of serum biochemical indices assayed except urea, calcium, potassium and chloride showed no significant (p > 0.05 differences among the treatment groups. It was therefore concluded that Samsorg 17 and ICSV 400 sorghum varieties could sustain local turkey production without any on toward effects on their haematological and serum biochemical indices.

  18. Effect of ascorbic acid on morphological and biochemical parameters in tomato seedling exposure to salt stress

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    Krupa-Małkiewicz Marcelina

    2015-06-01

    Full Text Available The aim of this study is to determine the effect of both NaCl and KCl alone and in comparison to AsA on the morphological and some biochemical parameters of Oxheart and Vilma cultivars of tomato under laboratory and field conditions. A combination of salt applied in the laboratory experiment caused a significant effect on seed germination and root and shoot length and a significant reduction of Chl a, Chl b and Car contents in 14-day-old tomato seedlings. However, seedlings of cultivar Vilma were characterised by higher tolerance to applied salt stress.

  19. Effect of Cadmium and Zinc on growth and Biochemical Parameters of Selected Vegetables.

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    Davika Bhargava

    2013-05-01

    Full Text Available Heavy metals occur naturally in soil but more is being released into the environment by humans especially from mining. Heavy metals are helpful for plants in mineral nutrition but in excess they cause pollution and are harmful for plants, invertebrates and some vertebrate fishes. Through precipitation of their compounds or by ion exchange into soil heavy metal pollutants can localize and lay dormant. They have inhibitory effects on plant growth and the performance of photosynthetic apparatus of plants. The present paper reports the adverse effects of zinc and cadmium on growth and biochemical parameters of Lady’s finger and Cluster bean.

  20. Effect of fire smoke on some biochemical parameters in firefighters of Saudi Arabia

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    Rezq Ameen M

    2008-12-01

    Full Text Available Abstract Background Firefighters who are facing fires, are frequently exposed to hazardous materials including carbon monoxide, hydrogen cyanide, hydrogen chloride, benzene, sulphur dioxide, etc. This study aimed to evaluate some relevant serum biochemical and blood hematological changes in activity involved firefighters in comparison to normal subjects. Subjects and Methods Two groups of male firefighters volunteered to participate in the study. The first included 28 firefighters from Jeddah, while the second included 21 firefighters from Yanbu, with overall age ranged 20–48 years. An additional group of 23 male non-firefighters volunteered from both cities as normal control subjects, of age range 20–43 years. Blood samples were collected from all volunteer subjects and investigated for some relevant serum biochemical and blood hematological changes. Results The results obtained showed that, there were statistically significant differences in liver function, kidney function, serum lipid profile, cortisol, creatine kinase, lactate dehydrogenase, iron and its biologically active derivatives, and blood picture in firefighters as compared with the normal control group. These results indicate that, fire smoke mainly affects serum biochemical and blood hematological parameters. Such results might point out to the need for more health protective and prophylactic measures to avoid such hazardous health effects that might endanger firefighters under their highly drastic working conditions. Conclusion Besides using of personal protective equipments for firefighters to protect them against exposure to toxic materials of fire smoke, it is recommended that, firefighters must be under continuous medical follow up through a standard timetabled medical laboratory investigations to allow for early detection of any serum biochemical or blood hematological changes that might happen during their active service life and to allow for early treatment whenever

  1. Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

    OpenAIRE

    Rasolomboahanginjatovo, Hasina Santatriniaina; Chorfi, Younès; Dupras, Raynald; Mills, Louis; Lefebvre, Réjean

    2014-01-01

    The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured...

  2. Effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats

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    Ana Angélica Henrique Fernandes

    2009-02-01

    Full Text Available Amongst the numerous co-adjuvant therapies which could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of naringerin on biochemical parameters in streptozotocin-induced (STZ - 60 mg/kg, i.p. diabetic rats. Male rats were divided into four groups: G1: untreated controls; G2: normal rats receiving naringerin; G3: untreated diabetics; G4: diabetics rats receiving naringerin. The naringerin (50mg/kg, i.p, decreased the hyperglycaemia and hyperlipidaemia associated with STZ-diabetes. The concentrations of serum insulin in treated diabetic rats tended to be increased. Naringerin treatment prevents STZ-induced changes in the activities of ALT, AST and LDH in the liver and heart, indicating the protective effect of naringerin against the hepatic and cardiac toxicity caused by STZ. The glycogen level in cardiac and hepatic tissues elevated with naringerin in diabetic rats. The naringerin can improve the glucose and lipid metabolism and is beneficial in preventing diabetic complications.Dentre as numerosas terapias para minimizar as complicações diabéticas, os antioxidantes e flavonoides são testados na clínica médica. Foi analisado o efeito da naringerina sobre os parâmetros bioquímicos em ratos diabéticos induzidos por estreptozotocina (STZ - 60mg/kg, i.p.. Ratos machos foram divididos em 4 grupos: G1: controle não tratado; G2: ratos normais que receberam naringerina; G3: diabéticos não tratados; G4: ratos diabéticos que receberam naringerina. Naringerina (50mg/kg, i.p., decresceu a hiperglicemia e a hiperlipidemia em ratos diabéticos. A concentração sérica de insulina em ratos tratados tendeu aumentar. A naringerina preveniu as alterações, provocadas pela estreptozotocina, na atividade hepática e cardíaca de ALT, AST e LDH, indicando o efeito protetor da naringerina sobre estes tecidos, contra toxicidade

  3. Influence of Fasciola hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

    Science.gov (United States)

    Hodžić, A; Zuko, A; Avdić, R; Alić, A; Omeragić, J; Jažić, A

    2013-01-01

    Background The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver. Methods Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n = 34) and infected group (n = 29). For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used. Results Biochemical analysis of tested parameters showed a significant elevation (P≤0.05) of serum gamma-glutamyl transferase (GGT), total bilirubin (TBIL) and direct bilirubin (DBIL) in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST) between groups. Vascular and biliary systems of the liver were found to be affected. Conclusion Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica. PMID:23682266

  4. Flaxseed bioactive compounds change milk, hormonal and biochemical parameters of dams and offspring during lactation.

    Science.gov (United States)

    Troina, A A; Figueiredo, M S; Passos, M C F; Reis, A M; Oliveira, E; Lisboa, P C; Moura, E G

    2012-07-01

    We evaluated maternal intake of SDG (secoisolariciresinol diglucoside), a compound from flaxseed, and flaxseed oil+SDG on biochemical and hormonal parameters of dams and male and female offspring during lactation. Dams were fed a standard diet (C); diet added 40 mg of SDG/100g diet (SDG) or diet added 40 mg of SDG/100g diet and 7% of flaxseed oil (OLSDG). SDG and OLSDG dams showed hyperprolactinemia. The OLSDG milk had lower lactose and protein, while the SDG milk had lower protein on the 14th day of lactation. At 14 days, OLSDG male and female pups showed lower body mass, SDG and OLSDG male pups had hypoprolactinemia and lower body fat mass, but higher visceral fat mass (VFM) and hypertriglyceridemia. At 21 days, male SDG and OLSDG presented hypotriglyceridemia. At 14 days, SDG and OLSDG female offspring showed higher serum 17-β estradiol (E2); OLSDG presented hypercholesterolemia and SDG presented hypertriglyceridemia. At 21 days, SDG and OLSDG female pups showed hypotriglyceridemia and OLSDG shower lower E2. Both maternal treatments changes maternal metabolism as well as hormonal and biochemical parameters of the offspring, which are gender-dependent. Maternal hyperprolactinemia may act as an imprint factor responsible for the hormonal and metabolic changes observed in the pups.

  5. Observed Changes in Some Biochemical Parameters After Magnetic Field Exposure to 1,5 Tesla

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    Turan Akdağ

    2012-10-01

    Full Text Available Aim: In daily life, individuals are exposed to electromagnetic contamination at different levels. Magnetic resonance imaging (MRI is a radiodiagnostic imaging technique using non-ionizing radiofrequency radiation. Considering possible adverse effects of magnetic fields may occur at long time, complications are difficult to determined earlier. Therefore, the effects of MRI on some biochemical parameters were evaluated before and after MRI.Material and Method: In the present study, applying for MRI and with no systemic disorders, total 40 volunteers between the ages of 25-45 were enrolled. Blood samples were investigated before and after MRI, and subjects were exposed to magnetic field of 1.5 T for 30 min. Blood samples were soon centrifuged and studied in terms of the levels of serum Ca, Fe, Fe binding, triglyceride, cholesterol, TT3, TT4, free T3, free T4, TSH, ferritin, insulin, lipase, HDL, LDL and glucose. Results: In the study, levels of Ca, cholesterol, triglyceride, HDL, TT3, Fe, Fe binding, lipase, TSH and insulin after MRI were found to be lower than those before the procedure. This changs was observed to be statistically significant(P<0.05, and the difference related to levels of LDL, glukoz, ferritin, Free T3, Free T and TT4 were found to be statistically insignificant. Discussion: It was concluded that some biochemical parameters ocur after MRI, and further studies are needed to evaluate longterm consequences and biological effects and whether the changes are temporary.

  6. Influence of Fasciola Hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

    Directory of Open Access Journals (Sweden)

    A Hodžić

    2013-03-01

    Full Text Available Background: The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver.Methods: Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n=34 and infected group (n=29. For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used.Results: Biochemical analysis of tested parameters showed a significant elevation (P≤0.05 of serum gamma-glutamyl transferase (GGT, total bilirubin (TBIL and direct bilirubin (DBIL in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST between groups. Vascular and biliary systems of the liver were found to be affected.Conclusion: Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica.

  7. Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

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    Frelich Angela

    2008-09-01

    Full Text Available Abstract D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.

  8. Risk of prostate cancer and its correlation with different biochemical parameters in non diabetic men

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    Neha Sharma

    2013-08-01

    Full Text Available Background: It has been hypothesized that men with long term diabetes have a lower risk of prostate cancer then non-diabetic men. Whether diabetes influences level of biomarkers such as prostate specific antigen (PSA, which is involved in the detection of prostate cancer is, unknown. In view of the aforementioned controversial literature, it was decided to evaluate this relation-ship in non-diabetic men. We evaluated the correlation between fasting glucose, prostate specific antigen and different biochemical lipid profile parameters with serum uric acid and serum creatinine in non-diabetic male between age group 40-61 years. Methods: Association between fasting serum glucose , different lipid parameters, serum uric acid, serum creatinine and prostate specific antigen in 83 non-diabetic males aged 40 to 61years were studied retrospectively. Glucose and lipid parameters and serum creatinine, serum uric acid were measured on fully automated analyser using standard reagent kits. Serum prostate specific antigen was measured by TOSOH-AIA-360, immunoassay method. Results: Correlations between different biochemical parameters were determined. Prostate specific antigen were negatively correlated with HDL (r= -0.22, p= 0.03 in age group 40-61 years. At the same fasting blood sugar were correlated positively(r= 0.34, p= 0.02 with prostate specific antigen in age group 51-60 years , but not in age group 40-50 years. Conclusion: We concluded that serum HDL (high density lipoprotein was negatively associated and FBS (fasting blood sugar was positively associated with risk of prostate cancer. We also suggest that in men of this age group a low HDL level should not be ignored while assessing prostate cancer risk especially if accompanied with an elevated FBS level even in the upper normal range. [Int J Res Med Sci 2013; 1(4.000: 476-481

  9. Potential of liquid extracts of Sargassum wightii on growth, biochemical and yield parameters of cluster bean plant

    Institute of Scientific and Technical Information of China (English)

    N Vijayanand; S Sivasangari Ramya; S Rathinavel

    2014-01-01

    Objective:To explore biofertilizing efficiency of seaweed liquid extracts of brown alga Sargassum wightii (S. wightii) on growth, biochemical and yield parameters of Cyamopsis tetragonoloba (C. tetragonoloba). Methods: Seaweeds were made to coarse powder and stock solution was prepared. Different concentrations such as 0.5%, 1.0%, 1.5%, 2.0%, 2.5%and 5.0%were prepared and given as foliar spray. Results: Seaweed Liquid Extract (SLE) at low concentration (1.5%) exhibited promoting effect on growth and yield parameters. Differential responses in the content of photosynthetic pigments, protein, reducing sugar, ascorbic acid and in the activity of nitrate reductase were also observed in the leaves of SLE treated seedlings when compared to untreated seedlings. Higher concentrations (above 1.5%) of SLE were found to show inhibitory effect. Conclusion:The presence of micro and macro nutrients, vitamins, growth hormones and other constituents in the seaweed extract might be very much useful to the crops but their level should be appropriate to enhance growth and productivity. It may be concluded that liquid seaweed extracts could serve as cost effective eco-friendly product for sustainable agriculture.

  10. Microbial Succession And Biochemical Aspects Of Mandai Fermentation At Low Salt Concentration.

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    Hasrul Satria Nur

    2009-04-01

    Full Text Available Mandai isfermented food that traditionally made from the flesh of jack fruit (Arthocarphus champeden Spreg.. Usually mandai ismade as high salt concentration. However, the objective of this research is to study the succession and biochemicalaspects of microbials during fermentation at low salt concentration (10% w/v for 14 days. During the period offermentation microbial cell numbers and biochemical aspects were observed at the 3rd, 5th, 7th and 14th day. Total cellnumber of bacteria and yeast were measured by pour plate method. The measurement was also conducted on fleshbefore incubation. The biochemical aspect observation included the contents of reducing sugar, N-total, salt, and pH.The research results indicated that the pattern of microbial succession occurred the fermentation. Yeast cells grewdominantly (2.8 x 109 CFU/g on the 5th day fermentation but bacteria were dominant at the end (1.1 x 107 CFU/g. Thehighest decrease of reducing sugar and N-total contents were 0.240% at the 14th day and 0.159% at the 5th day,respectively. However, salt concentration was relatively stable and pH was varied within the range of 3.71-6.12 for thewhole period fermentation.

  11. RELATIONSHIP BETWEEN BIOCHEMICAL PARAMETERS - LIVER ENZYMES (AST, ALT AND ALP AND BLOOD PHENYTOIN LEVELS IN PATIENTS OF EPILEPSY

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    Ashok

    2013-11-01

    Full Text Available ABSTRACT: CONTEXT: Mankind exhibits epilepsy as one of the common neurologic disorders. Standard drug therapy of this disease provides control of seizures in more than 80% of patients. Effective, specific treatment by Anti - Epileptic Drugs becomes absolutely essential for successful treatment in human subjects. Epilepsy especially Grand - Mal variant is typically treated with Phenytoin. Unique point about our study is focused on the estimat ion of serum phenytoin sodium concentration and to correlate it with some of the important biochemical markers that assess the hepatic function. AIMS: 1. It may help to assess whether the epileptic patient receiving phenytoin on an out - patient basis requir es an additional dose when monitoring shows suboptimal levels. 2. Should the maintenance dose be reduced, stopped or adjusted to minimize the dose related adverse effects without sacrifice of seizure control. 3. Study also provides an estimation of out - pat ient compliance. 4. Rational designing of dosing regimen, prediction of future drug concentration and assessment of functional impairment due to disease may be possible. 5. Failure to provide adequate control in maximal tolerated dose by phenytoin after co nfirming compliance may give a clue to substitute another drug. METHODS AND MATERIALS: Thirty seven patients suffering from grand mal epilepsy who attended the neurology OPD of Basaveshwar Teaching and General Hospital were selected for the study. Two pati ents received 100mg, 25 patients 200mg and 10 patients received 300mg of Dilantin Sodium (Parke Davis capsule daily at night between 9 - 10pm. The period of the exposure to the drug varied from 1 - 5 years. Ten healthy volunteers of same age group who were no t receiving any drugs were the control for the study. 10ml of blood was collected from each patient between 9 - 10am. 2ml of serum was extracted from each sample; serum phenytoin level was measured using UV Spectrophotometer, Bausch and Lomb

  12. Effect of gender, biochemical parameters & parathyroid surgery on gastrointestinal manifestations of symptomatic primary hyperparathyroidism

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    Viral N Shah

    2014-01-01

    Full Text Available Background & objectives: Information on gastrointestinal manifestations and then response after curative parathyroid surgery is scarce in symptomatic primary hyperparathyroidism (PHPT. This study was carried out to analyse gastrointestinal manifestations in patients with PHPT and their associations with biochemical parameters. Methods: This retrospective study included 153 patients with symptomatic primary hyperparathyroidism (PHPT. The signs and symptoms pertaining to gastrointestinal system were analyzed. The difference of symptoms between men and women and difference in biochemical parameters in presence of different symptoms were evaluated. The relationship between serum calcium, phosphate and parathyroid hormone (PTH levels with presence of gallstone and pancreatitis was also studied. Result: Of the 153 patients, 46 (30% were men. The mean age was 39.2 ± 13.9 yr. Nearly 80 per cent of PHPT patients had at least one symptom/ sign related to gastrointestinal system. The most common gastrointestinal manifestations were abdominal pain 66 (43%, constipation 55 (36%, and nausea/or vomiting 46 (30%. Nearly one-fourth 34 (22% of patients had a history of either gallstone disease or cholecystectomy or both. The prevalence of gallstone disease was higher in women (P<0.05. Imaging and biochemical evidence of pancreatitis was found in 27 (18% patients. Pancreatitis was more common in men compared to women (P<0.05 despite the higher prevalence of gallstones in women. Serum calcium, phosphate or PTH levels were not associated with high risk for gallstone disease, however, serum calcium (P<0.05 was associated with 1.3 times higher risk of developing pancreatitis. In majority of patients, gastrointestinal manifestations resolved within three months of curative parathyroidectomy. Except two patients, none had recurrence of pancreatitis. Interpretation & conclusions: The study revealed that the gastrointestinal symptoms were common in patients with

  13. The dynamics of biochemical parameters in blood of clinically healthy holstein cows from day 5 before to day 60 after calving

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    Irena Celeska

    2015-10-01

    Full Text Available The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and BHBA. Milk production and body condition score were also estimated. Obtained results showed that cows were exposed to mild to marked metabolic distress. Energy status was changed due to increased values of NEFA and BHBA and decreased value of glucose after calving. Protein concentrations were increased at day 10 after calving, despite the decrease of the level of albumin. Urea concentrations before and after calving were within physiological range indicating an optimal protein diet. Increased values of total bilirubin at day 5 after calving indicated liver increased activity. Lipid status presented by triglycerides and total cholesterol revealed no differences in blood concentrations. Milk production was highest at day 30 after calving. BCS were highest in dry cows, thereafter they declined and recovered at day 60 after calving. In conclusion, biochemical parameters can be used as relevant indicators of metabolic distress in cows around calving with milk and BCS recording as aside parameters. Changes in some biochemical parameters indicate liver increased activity and metabolic stress, that could lead to decreased milk production, impaired reproductive performance and, finally, to illness.

  14. Establishment of reference interval for liver specific biochemical parameters in apparently healthy north Indian population.

    Science.gov (United States)

    Yadav, Dharamveer; Mishra, Sandhya; Gupta, Monika; John, P J; Sharma, Praveen

    2013-01-01

    Reference intervals (RI) are the most common decision support tool used for interpretation of numerical laboratory reports. The quality of the RI can play as large a role in result interpretation as the quality of the result itself. As such there is hardly any study examining RI for liver specific biochemical parameters in Indian population especially north Indians having drastically different food habits as compared to rest of the India. So there is a need to establish the RI for north Indian population. Present study was conducted on 2,021 apparently healthy individuals of north Indian origin ranging in age from 15-60 years, were selected randomly using defined criteria. Lipemic, hemolysed, icteric and stored samples were also excluded adopting preanalytical criteria for rejection of sample. Non parametric methodology for determination of RI was adopted as most of the biochemical parameters included revealed non Gaussian distribution. Data were analyzed for middle 95 percentile (2.5th-97.5th percentile), median and 95 % confidence interval using SPSS software package version 10.0. The upper and the lower limit of RI (reported Vs observed) for bilirubin (0-1.2 Vs 0.30-1.30 mg/dL), serum glutamate oxaloacetate transferase (SGOT) (0-41 Vs 13-52.80 IU/L), serum glutamate pyruvate transferase (SGPT) (0-50 Vs 10-68 IU/L) showed wide variation as compared to reported standard RI however Gamma glutamyl transferase (GGT) (0-50 Vs 5.00-50.60 IU/L) remained within the reported standard RI. Further gender wise evaluation revealed higher cutoff in males (AST 14-55, ALT 11-70.35, GGT 6.76-51.09 in IU/L, bilirubin (0.40-1.34 mg/dL) as compared to females (SGOT 13-50.43, SGPT 9-63.43, GGT 3.92-48.70 in IU/L, Bilirubin 0.30-1.20 mg/dL) for both enzymatic and non enzymatic biochemical parameters. The variations may be attributed to dietary pattern smoking and alcoholism.

  15. Changes in biochemical parameters on the first day after kidney transplantation: risk factors for nosocomial infection?

    Institute of Scientific and Technical Information of China (English)

    YANG Yi; REN Liang; ZHANG Yong; LIU Hang; CAO Bin; ZHANG Xiao-dong

    2010-01-01

    Background Nosocomial infection in early post-transplantation period is a tough problem for kidney transplantation. Few reports have explored the relations between biochemical parameters and nosocomial infection in kidney transplantation. This retrospective study was carried out to describe the characteristics of nosocomial infection in the very early period of kidney transplantation and to determine the risk factors in biochemical parameters and their alterations. Methods Patients who underwent their first kidney transplantation from January 2001 to March 2009 in Beijing Chao-Yang Hospital were recruited and the nosocomial infectious episodes were collected for this study. Gender, age, donor type, delayed graft function (DGF) and biochemical parameters such as serum uric acid, lipids files and albumin on day 0 (before transplantation) and day 1 (24 hours after transplantation) and their changes were analyzed with Logistic regression models for nosocomial infection. Results A total of 405 patients (315 men and 90 women) were involved in this study. There were 80 patients experiencing 113 infection episodes and 105 strains of microorganism were indentified. In univariate analysis, there were significant differences in DGF, albumin on day 0, lipoprotein (a) (Lp(a)) on day 1, change in low density lipoprotein-cholesterol (LDL-C, day 1-day 0) and change in uric acid (day 1-day 0) between nosocomial infection patients and noninfectious patients (P<0.05). In multivariate analysis, change in uric acid (day 1-day 0) (Off 5.139, 95% Cl 1.176-22.465, P<0.05), change in LDL-C (day 1-day 0) {OR4.179, 95% Cl 1.375-12.703, P<0.05) and DGF (Of? 14.409, 95% Cl 1.603-129.522, P<0.05) were identified as independent risk factors for nosocomial infection in kidney transplantation. Conclusions Most nosocomial infections in early postoperative period of kidney transplantation are bacterial, especially with Gram-negative bacteria. The most common infection sites are respiratory tract

  16. NOVEL INVESTIGATIONS ON THUJA OCCIDENTALIS EXTRACT ON RABBIT HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

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    Mansoor Ahmad

    2013-03-01

    Full Text Available Thuja occidentalis (family: Cupressaceae has been used as antiseptic, expectorant, diuretic and in the treatment of cancer and removal of kidney stones in different systems of medicine. The present studies were carried out on T. occidentalis leaves extract to evaluate the hematological and biochemical effects on rabbits. The animals were divided in to control (A and B and treated (C and D male and female groups (n=5. There was a slight decrease in Hemoglobin, Red blood cell count and Hematocrit/Packed cell volume values, while a significant increase in platelet count was observed in male and female treated groups. However, slight increase in total leukocyte count value in male treated group and decrease count with female treated group was also observed. Urea, Creatine phospho kinase, Creatine phospho kinase iso-enzyme, Direct Bilirubin, Gamma-glutamyl transpeptidase, Cholesterol and Albumin/Globulin ratio values were elevated in male treated group. While Phosphorus, Uric acid, Lactate dehydrogenase, Serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, Alkaline Phosphatase, Cholesterol, Triglycerides, High density lipoprotein, Low density lipoprotein, Very-low-density lipoprotein, Globulin and Blood Glucose values were found low. In female treated group almost same results were observed like male treated group except that hypoglycemic effect was more pronounced in female treated group. These findings suggested that when results of hematological and biochemical parameters of test groups (C & D were compared with their respective controls (A & B, significant changes were observed which contributes towards the diversified therapeutic effect of T. occidentalis.

  17. Contaminant concentrations, biochemical and hematological biomarkers in blood of West Indian manatees Trichechus manatus from Brazil.

    Science.gov (United States)

    Anzolin, D G; Sarkis, J E S; Diaz, E; Soares, D G; Serrano, I L; Borges, J C G; Souto, A S; Taniguchi, S; Montone, R C; Bainy, A C D; Carvalho, P S M

    2012-07-01

    The West Indian manatee Trichechus manatus is threatened with extinction in Brazil, and this study focused on nondestructive blood samples analyzed for metals, polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), as well as biochemical and hematological biomarkers. Studied manatees were kept at Projeto Peixe-Boi headquarters in Pernambuco State, and at two natural areas in estuaries where they are released to the wild. Manatees kept at the natural estuary in Paraiba State have blood concentrations of Al, Pb, Cd, Sn that are 11, 7, 8 and 23 times greater, respectively, than the concentrations found in blood of animals from the same species in Florida, USA. An inhibition of butyrylcholinesterase in manatees kept at the two reintroduction sites in Alagoas and Paraiba States indicated possible exposure of the animals to cholinesterase inhibitor insecticides. PCBs and OCPs were not detected. Results from this study will help delineate conservation efforts in the region.

  18. Dietary effects of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Yeganeh, Sakineh; Teimouri, Mahdi; Amirkolaie, Abdolsamad Keramat

    2015-08-01

    This study evaluated the effects of diets containing 0, 2.5, 5, 7.5 and 10% of Spirulina platensis on hematological and serum biochemical parameters of rainbow trout (Oncorhynchus mykiss). Fish (n=180; 101±8 g) were randomly divided into fifteen 300 L fiberglass tanks in triplicates for a period of ten weeks. The RBC, WBC, hemoglobin, total protein and albumin levels increased significantly in the groups supplemented with S. platensis. Dietary inclusion of S. platensis had no significant effects on hematocrit, cholesterol, triglyceride and lactate of the blood. HDL-cholesterol was larger in rainbow trout fed 10% S. platensis in comparison with the other diets, whereas LDL-cholesterol significantly decreased with increasing of S. platensis inclusion. Cortisol and glucose significantly decreased with increasing of S. platensis inclusion. The present results demonstrate that inclusion of 10% S. platensis can be introduced as an immunostimulant in rainbow trout diets.

  19. Correlation between biochemical parameters and susceptibility of freshwater fish to malathion.

    Science.gov (United States)

    Li Shao-Non; Fan De-Fang

    1996-07-01

    Acute toxicity (96-h LC50) of malathion was tested using five species of freshwater fish, namely, topmouth gudgeon (Pseudorasbora parva), goldfish (Carassius auratus), nile tilapia (Tilapia nilotica), mosquitofish (Gambusia affinis), and rainbow trout (Salmo gairdneri). Correlation was found between susceptibility and biochemical parameters such as activity of brain acetylcholinesterase (AChE) and in vitro resistance of the enzyme to inhibition (IC50) of malaoxon (a major metabolite of malathion). The in vitro study also showed that malaoxon instead of malathion was the main inhibitor of AChE. Susceptibility to malathion was considerably changed as the fish was pretreated with piperonyl butoxide (PB, a P-450 inhibitor) and triphenyl phosphate (TPP, an inhibitor of carboxylesterase), respectively. Toxicity of malathion was significantly increased by TPP, but the responses of fish to PB were quite different among species. This suggested that both carboxylesterase and monooxygenase played an important role in susceptibility determination, and great variations existed among species in activity of monooxygenase.

  20. Comparison of biochemical parameters of rainbow trout (Oncorhynchus mykiss) reared in two different trout farms'

    Science.gov (United States)

    Karatas, Tayfun

    2016-04-01

    The aim of this study was to compare biochemical parameters of cultured rainbow trouts (Oncorhynchus mykiss, Walbaum, 1972) reared in two different trout farms' (Agri and Erzurum). The average weights of fish were 150±10gr for first station (Agri), 230±10gr for second station (Erzurum). Fishes used in research were randomly caught from pools, and fifteen pieces were used for each group. Fishes were fed with commercial trout feed with 45-50% crude protein twice a day. The levels of AST, ALT, LDL, total cholesterol and triglyceride in the second station (Erzurum) were found to be higher (pHDL in the second station (Erzurum) were found to be lower (pHDL, LDL, triglyceride may be associated with size, sex, sexual maturity and environmental conditions (temperature, pH, hardness and dissolved oxygen).

  1. THE STUDY OF SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH MULTIPLE MYELOMA

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    Bularda Morozan Mihai

    2010-09-01

    Full Text Available This paper presents the results of some biochemical parameters obtained in the laboratory of “Elena Beldiman” Emergency Hospital Barlad, and in the”Dr. Stoian –Dr. Ungureanu”Private Medical Practice on 10 patients diagnosed with multiple myeloma during 2005-2011. Numerous researches over serum proteins with the help of various methods ( refractometry have shown the presence of some changes in protein fractions of blood serum. These changes express the change of the normal ratio between these fractions, that means disorders in colloidal structure of blood. The results of the electrophoresis analysis, also in accordance with those from the scholarly literature, show that the changes in the condition of the blood serum are due to the increase in the globulins fractions which is linked to the growth of the immunological processes, the antibodies synthesis being especially closely linked to gammaglobulinic fraction

  2. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss Fry

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    Salman Rauoof CHALKOO

    2013-01-01

    Full Text Available This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocytes (WBC and erythrocytes counts (RBC, hemoglobin (Hb content, hematocrits (PCV, leukocytes differential count and blood indices such as MCV, MCH and MCHC. Also blood serums were analysed for total protein (TP and blood enzymes. All the calculations were made using the SPSS© and t-test statistical method. In hematological findings nine parameters were revealed significant differences (P

  3. Ameliorative effects of L-carnitine and vitamin E (α-tocopherol) on haematological and serum biochemical parameters in White Leghorn cockerels given ochratoxin A contaminated feed.

    Science.gov (United States)

    Abidin, Z; Khan, M Z; Khatoon, A; Saleemi, M K; Khan, A; Javed, I

    2013-01-01

    1. L-carnitine is a quaternary ammonium compound biologically synthesised from the amino acids methionine and lysine while vitamin E (α-tocopherol) is an important antioxidant. The objective of the present study was to evaluate the ameliorative effects of L-carnitine and vitamin E upon haematological and serum biochemical parameters in ochratoxin A intoxicated birds. 2. Day-old White Leghorn cockerels were acclimatised for 2 d, divided in 12 groups with 20 birds in each group. From d 3 of age, they were given different combinations of ochratoxin A (1.0 and 2.0 mg/kg), L-carnitine (1 g/kg) and vitamin E (200 mg/kg) in feed. Haematological (erythrocyte count, leucocyte count, haemoglobin concentration and haematocrit percentage) and serum biochemical parameters (serum urea, creatinine, albumin, total proteins and alanine aminotransferase) were evaluated. 3. Results confirmed that L-carnitine and vitamin E given alone or combined with 1.0 mg/kg ochratoxin A ameliorated toxin induced alterations in haematological and serum biochemical parameters. This amelioration, however, did not occur when ochratoxin of 2.0 mg/kg was given. 4. L-carnitine and vitamin E in combination have the ability to ameliorate ochratoxin altered haematological and serum biochemical parameters. However, the optimum ratio of L-carnitine + vitamin E, to be used to assure such mitigation of ochratoxin A altered changes in haematological and serum biochemical parameters in cockerels, has yet to be determined. The combination used in this study was indeed sufficient to ameliorate the alterations induced by ochratoxin A up to 1.0 mg/kg feed.

  4. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

    Science.gov (United States)

    Ramanand, Sunita J.; Ramanand, Jaiprakash B.; Ghongane, Balasaheb B.; Patwardhan, Milind H.; Patwardhan, Varsha M.; Ghanghas, Ravi; Halasawadekar, Nimish R.; Patil, Praveenkumar

    2014-01-01

    Background: Polycystic ovary syndrome (PCOS) is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS). Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria) Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results: The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45). Hypoadiponectinemia (serum adiponectin <4 μg/mL) was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027). Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56). It was significantly lower in patients with acanthosis nigricans (AN) as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038). Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57). A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH), thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH), LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN) of insulin resistance. PMID:24741521

  5. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

    Directory of Open Access Journals (Sweden)

    Sunita J Ramanand

    2014-01-01

    Full Text Available Background: Polycystic ovary syndrome (PCOS is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS. Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results : The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45. Hypoadiponectinemia (serum adiponectin <4 μg/mL was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027. Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56. It was significantly lower in patients with acanthosis nigricans (AN as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038. Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57. A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH, thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH, LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN of insulin resistance.

  6. ESTIMATION OF BIOCHEMICAL AND HAEMA TOLOGICAL PARAMETERS AFTER TREATMENT WITH BIOVET IN DIFFERENT STRAINS OF LAYING HENS

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    Bashir Mahmood Bhatti, Tanzeela Talat, Rozina Sardar and Ghazala Naheed

    2002-04-01

    Full Text Available This experiment was conducted to study 'effect of Biovet (E.M. Technology on biochemical and haematological parameters of four genetically 'different strains of laying hens including cross, Desj, Fayoumi and Nick Chick. A total of 60 laying hens were involved in the trial giving 15 birds under each strain. Prior to administration of Biovet (2 ml/litre} birds were maintained in,controlled environment for 2 weeks, five blood samples were taken from each strain, and analysed for bichemical and haematological parameters, The same birds were then administered Biovet in drinking water and after 2 weeks of treatment, five blood samples from each strain of chickens were collected and teste4:as was done before treatment. Serum glucose was markedly (P<0.05 increased due to treatment and there was also significant (P<0.05 inter strain variation. Serum protein levels were only affected (P<0.05 due to treatment but there was no inter strain variation, Serum albumin and cholesterol were not affected by treatment, The haematological parameters such as Hb, WBC, Eosinophils, Lymphocytes,PCV , MCH, MCHC; and Heterophils were not affected. by treatment. There was significant (P<0.05 difference in RBCs concentration in serum which also; caused an -increase (P<0.05 in MCV values. The Biovet supplementation presumably stimulated haemopoietic system to improve performance of birds.

  7. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).

    Science.gov (United States)

    Binaii, Mohammad; Ghiasi, Maryam; Farabi, Seyed Mohammad Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

    2014-01-01

    The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle did not significantly change the mean cell volume, mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and hematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing 6% and 12% nettle increased the WBC and MCHC compared to the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the 6% and 12% nettle diet compared to the other groups. The results suggest that by using this herb there will be an improvement in hemato-biochemical parameters and immune function of juvenile beluga.

  8. Concentration of field and skim latex by microfiltration - membrane fouling and biochemical methane potential of serum.

    Science.gov (United States)

    Thongmak, Narumol; Sridang, Porntip; Puetpaiboon, Udomphon; Grasmick, Alain

    2015-01-01

    Cross-flow microfiltration was used to concentrate field and skim latex suspensions and recover the smallest compounds (proteins, sugars, etc.) in permeate (serum solutions). The experiments were performed in a lab-scale microfiltration unit equipped with ceramic membranes. In continuous mode, the operations were performed at constant trans-membrane pressure (0.5 bars), constant cross-flow velocity (3 m/s) and constant temperature (28 ± 2°C). In retentate, the volumetric concentration factor was only close to 2 (about 54% of total solid content, TSC) when concentrating the field latex suspensions, and it reached 10 (close to 40% TSC) when concentrating skim latex suspensions. The quality of retentate suspensions let envisage a significant potential of industrial valorization. The membrane fouling rates appeared as an increasing function of dry rubber content suspension, and the main fouling origin (94%) was linked to a reversible accumulation of suspended compounds on the membrane surface. Permeate appeared as a clear yellow solution containing the smallest soluble organic fractions that show a high degree of biodegradability when using biochemical methane potential tests. The chemical oxygen demand (COD) removal was then higher than 92% and the methane production yield was close to 0.29 NLCH4/gCODremoved. The association of a membrane separation step and anaerobic digestion appeared, then, as a relevant solution to recover rubber content from skim latex suspensions and energy from the anaerobic digestion of serum.

  9. Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).

    Science.gov (United States)

    Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

    2014-11-01

    The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality.

  10. The effects of Cosmos caudatus (Ulam Raja) supplementation on bone biochemical parameters in ovariectomized rats.

    Science.gov (United States)

    Mohamed, Norazlina; Yin, Chai Mei; Shuid, Ahmad Nazrun; Muhammad, Norliza; Babji, Abdul Salam; Soelaiman, Ima Nirwana

    2013-09-01

    Cosmos caudatus (ulam raja) contains high mineral content and possesses high antioxidant activity which may be beneficial in bone disorder such as postmenopausal osteoporosis. The effects of C. caudatus on bone metabolism biomarkers in ovariectomized rats were studied. 48 Sprague-Dawley rats aged three months were divided into 6 groups. One group of rats was sham-operated while the remaining rats were ovariectomized. The ovariectomized rats were further divided into 5 groups: the control, three groups force-fed with C. caudatus at the doses of 100mg/kg, 200mg/kg or 300mg/kg and another group supplemented with calcium 1% ad libitum. Treatments were given 6 days per week for a period of eight weeks. Blood samples were collected twice; before and after treatment. Parameters measured were bone resorbing cytokine; interleukin-1 and the bone biomarkers; osteocalcin and pyridinoline. Serum IL-1 and pyridinoline levels were significantly increased in ovariectomized rats. Supplementation of C. caudatus was able to prevent the increase of IL-1 and pyridinoline in ovariectomized rats. Besides that, C. caudatus showed the same effect as calcium 1% on biochemical parameters of bone metabolism in ovariectomized rats. In conclusion, Cosmos caudatus was as effective as calcium in preventing the increase in bone resorption in ovariectomized rats.

  11. Women with metabolic syndrome improve antrophometric and biochemical parameters with green banana flour consumption

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    Sandra Tavares da Silva

    2014-05-01

    Full Text Available Background: Consumption of green banana flour (GBF may promote health benefits, such as, decreased appetite, weight loss, glycemic control, intestinal function and lipid profile improvement, aging delay, cancer and heart disease prevention. The aim of this study was to evaluate the effects of green banana flour consumption on anthropometric and biochemical parameters in overweight women. Methods: The glycemic index of flour in the study was determined. The effects of consumption of 20 g of green banana flour/day on weight, body mass index (BMI, blood pressure, waist and hip circumference, body composition, hemoglobin, lipid profile, glucose, insulin, insulin resistance, liver function and energy intake were evaluated in 25 overweight women for 45 days. Results: The glycemic index of the flour under study was classified as low. Reduction (p < 0.05 in systolic blood pressure, hip circumference and fasting glucose levels were found in women who had metabolic syndrome criteria. Conclusions: Consumption of green banana flour (20 g/day for 45 days did not promote weight loss or changes in body composition in overweight women. It was noted, however, decreased hip circumference. Significant health parameter improvements were also noted in individuals with metabolic syndrome, which showed a reduction in systolic blood pressure and fasting glucose.

  12. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

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    Fabrícia Souza Predes

    2009-04-01

    Full Text Available This study was carried out to evaluate the effects of Arctium lappa L. (burdock on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST, alanine transaminase (ALT, gamma glutamyl transferase (GGT, total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.A bardana (Arctium lappa L é uma planta trazida do Japão e aclimatada no Brasil, e é extensamente utilizada na medicina popular em todo mundo. Este estudo foi realizado para avaliar os possíveis efeitos da A. lappa no fígado e nos parâmetros bioquímicos plasmáticos em ratos Wistar adultos. Estes receberam a infusão de bardana nas doses de 10 ou 20 g de folhas secas /L de água, por 40 dias. Não houve alteração significativa nos níveis plasmáticos de albumina, aspartato transaminase (AST, alanina transaminase (ALT, gamma glutamil transferase (GGT, proteínas totais, colesterol total, uréia, ácido úrico, triglicérides, cálcio, fósforo, bilirrubina direta e cloro. A análise morfológica não revelou alterações histopatológicas no fígado. Os dados bioquímicos e morfológicos não indicaram a toxicidade da bardana.

  13. Effects of Salinity on Leaf Spectral Reflectance and Biochemical Parameters of Nitrogen Fixing Soybean Plants (Glycine max L.)

    Science.gov (United States)

    Krezhova, Dora D.; Kirova, Elisaveta B.; Yanev, Tony K.; Iliev, Ilko Ts.

    2010-01-01

    Measurements of physiology and hyperspectral leaf reflectance were used to detect salinity stress in nitrogen fixing soybean plants. Seedlings were inoculated with suspension of Bradyrhizobium japonicum strain 273. Salinity was performed at the stage of 2nd-4th trifoliate expanded leaves by adding of NaCl in the nutrient solution of Helrigel in concentrations 40 mM and 80 mM. A comparative analysis was performed between the changes in the biochemical parameters - stress markers (phenols, proline, malondialdehyde, thiol groups), chlorophyll a and b, hydrogen peroxide, and leaf spectral reflectance in the spectral range 450-850 nm. The spectral measurements were carried out by an USB2000 spectrometer. The reflectance data of the control and treated plants in the red, green, red-edge and the near infrared ranges of the spectrum were subjected to statistical analysis. Statistically significant differences were found through the Student's t-criterion at the two NaCl concentrations in all of the ranges examined with the exception of the near infrared range at 40 mM NaCl concentration. Similar results were obtained through linear discriminant analysis. The tents of the phenols, malondialdehyde and chlorophyll a and b were found to decrease at both salinity treatments. In the spectral data this effect is manifested by decrease of the reflectance values in the green and red ranges. The contents of proline, hydrogen peroxide and thiol groups rose with the NaCl concentration increase. At 80 mM NaCl concentration the values of these markers showed a considerable increase giving evidence that the soybean plants were stressed in comparison with the control. This finding is in agreement with the results from the spectral reflectance analysis.

  14. Biochemical brain markers and purinergic parameters in rat CSF after seizure induced by pentylenetetrazol.

    Science.gov (United States)

    Oses, Jean Pierre; Leke, Renata; Portela, Luis Valmor; Lara, Diogo R; Schmidt, André P; Casali, Emerson André; Wofchuk, Susana; Souza, Diogo O; Sarkis, João José Freitas

    2004-09-30

    Cellular and molecular mechanisms involved in the generation of seizures and the magnitude of neural cells injury are not fully understood. We evaluated astrocyte and/or neuronal injury in rats in the pentylenetetrazol model of acute seizures by measuring S100B and NSE levels in cerebrospinal fluid. Additionally, we determined ADP and GDP hydrolysis by soluble nucleoside triphosphate diphosphohydrolase in the cerebrospinal fluid, and the concentration of nucleosides adenosine, inosine and guanosine as putative markers of brain injury. After pentylenetetrazol-induced seizures: (i) S100B values increased from 10 to 30 min, returning to control levels at 24 h; NSE levels presented a biphasic increase: an increase at 10 to 30 min returning to control levels, and again at 240 min followed by a decline at 24 h; (ii) nucleotidase activities increased from 10 min, returning to control levels at 240 min; (iii) guanosine and inosine levels increased exclusively after 30 min. In summary, this study showed biochemical changes in the cerebrospinal fluid occurring after seizures induced by pentylenetetrazol. Such events may have a modulating effect upon seizure expression, particularly nucleoside triphosphate diphosphohydrolase activities and nucleoside concentrations, but are nevertheless followed by neural death as evidenced by the increase in NSE and S100B levels.

  15. Toxicity of Neem Leaf Extracts (Azadirachta indica A. Juss on Some Haematological, Ionoregulatory, Biochemical and Enzymological Parameters of Indian Major Carp, Cirrhinus mrigala

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    M. Saravanan

    2011-10-01

    Full Text Available In the present study, the median lethal concentration (LC 50 of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT and glutamate pyruvate transaminase (GPT activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in environmental biomonitoring of neem based products in freshwater environment.Keywords: Azadirachta indica, Acute toxicity, Cirrhinus mrigala, Haematology, Ion regulation, Biochemical and Enzymological parameters.

  16. Effects of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854)

    Science.gov (United States)

    Zhu, Zhiming; Song, Bolan; Lin, Xiaotao; Xu, Zhongneng

    2016-01-01

    This study examines the effect of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb ( Barbonymus schwanenfeldii). Blood samples were taken on days 1, 23 and 45 from control fish and from two training groups maintained at current speeds of 0.06 bl/s (body length per second), 0.66 bl/s, and 1.92 bl/s, respectively. Significantly increased red-blood-cell counts and hematocrit were observed in the post-training groups on days 23 and 45. Significantly increased hemoglobin concentrations were also observed in the 1.92 bl/s group on days 23 and 45. In contrast, values of mean corpuscular volume were significantly lower in the 1.92 bl/s group than in the other groups on day 45. Nitroblue-tetrazolium-positive cells and lysozyme and superoxidase dismutase activities in the plasma increased significantly with increasing training intensity on days 23 and 45. Antibacterial activities were significantly increased in the trained groups compared with the control group on day 23; significantly elevated alkaline phosphatase activity was observed in the 1.92 bl/s groups on day 45. Therefore, training intensities of 0.66 and 1.92 bl/s enhanced the blood oxygen-carrying capability and plasma immune parameters of juvenile tinfoil barbs.

  17. Assessment of hemato-biochemical parameters on exposure to low level of deltamethrin in mouse model

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    Anita Tewari

    2014-03-01

    Full Text Available Aim: In this study, sub-acute toxicity of deltamethrin on hematological and biochemical blood parameters of male albino Swiss mice was evaluated. Materials and Methods: Generally, the maximum permissible residue level (MRL of deltamethrin for food products lies between 0.01 to 0.5 mg/kg body weight. So the mice were exposed orally with two doses of pesticide i.e. 0.1 and 0.5 mg/kg body weight. The doses were given on a daily basis for a period of 15 days and 30 days respectively. Ground nut oil was used as control treatment. Samples of blood were collected at the end of the treatment. Hepatotoxicity was evaluated by quantitative analysis of the serum enzymes alanine transaminase (ALT, aspartate transaminase (AST, alkaline phosphatase (ALKP, total bilirubin (TBIL and serum urea. Alterations of hematological parameters were analysed by total leukocyte, differential leukocyte count and hemoglobin levels. Results: Significant increase in the levels of hepatic enzymes (ALT, AST, ALKP were observed for both doses, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations for 0.5 mg/kg body weight dose which is indicated by leukocytosis, lymphocytosis and neutropenia in long duration study. Conclusions: The results indicated that even very low dose of deltamethrin can promote hematological and hepatic alterations. Thus it is imperative to do further studies on the detrimental effect of the low levels of pyrethroid commonly present in our food, which further necessitate the reduction of maximum permissible levels of residual synthetic pyrethroid levels in foods and feed.

  18. Intrahippocampal Infusion of Crotamine Isolated from Crotalus durissus terrificus Alters Plasma and Brain Biochemical Parameters

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    Rithiele Gonçalves

    2014-11-01

    Full Text Available Crotamine is one of the main constituents of the venom of the South American rattlesnake Crotalus durissus terrificus. Here we sought to investigate the inflammatory and toxicological effects induced by the intrahippocampal administration of crotamine isolated from Crotalus whole venom. Adult rats received an intrahippocampal infusion of crotamine or vehicle and were euthanized 24 h or 21 days after infusion. Plasma and brain tissue were collected for biochemical analysis. Complete blood count, creatinine, urea, glutamic oxaloacetic transaminase (GOT, glutamic pyruvic transaminase (GPT, creatine-kinase (CK, creatine kinase-muscle B (CK-MB and oxidative parameters (assessed by DNA damage and micronucleus frequency in leukocytes, lipid peroxidation and protein carbonyls in plasma and brain were quantified. Unpaired and paired t-tests were used for comparisons between saline and crotamine groups, and within groups (24 h vs. 21 days, respectively. After 24 h crotamine infusion promoted an increase of urea, GOT, GPT, CK, and platelets values (p ≤ 0.01, while red blood cells, hematocrit and leukocytes values decreased (p ≤ 0.01. Additionally, 21 days after infusion crotamine group showed increased creatinine, leukocytes, TBARS (plasma and brain, carbonyl (plasma and brain and micronucleus compared to the saline-group (p ≤ 0.01. Our findings show that crotamine infusion alter hematological parameters and cardiac markers, as well as oxidative parameters, not only in the brain, but also in the blood, indicating a systemic pro-inflammatory and toxicological activity. A further scientific attempt in terms of preserving the beneficial activity over toxicity is required.

  19. Investigating the Effect of Oral Consumption of Tear Assafoetida on Hepatic, Renal, Cardiac, and Blood Biochemical Parameters of Rats

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    M Esmailidehaj

    2013-12-01

    Methods: Forty male Wistar rats, with average weighting of 300 grams, were divided into five groups of eight in each. The first group was considered as control group and groups 2-5 as the pretreatment groups that were pre-treatmented by 12.5, 25, 50 and 100 mg/kg tear assafoetida for 30 days, respectively. Then, blood samples were collected for measurement of biochemical parameters of liver (AST, ALT, ALP, kidney (Urea, Cr, cardiac (CPK and blood coagulation (Pt, Ptt. Blood pressures were also recorded through inserting a cannula into the carotid artery. Lead II of electrocardiogram was used to monitor electrical activity of the heart. Data was analyzed by one-way analysis of variance. Results: The plasma level of AST, CPK and Ptt significantly reduced in pretreatment groups with assafoetida (P<0.05. With increasing the dose of assafoetida, plasma urea concentration reduced and plasma Cr concentration and all blood pressures increased insignificantly. Conclusion: The findings of this study suggest that low doses of tear assafoetida (25 mg/kg does not probably have any side effects and even maybe useful for health, but at higher doses (100 mg/kg it may lead to complications. Hence, there is a need for further studies to answer some questions that arose from this study.

  20. Effect of bioactive compounds extracted from euphorbious plants on hematological and biochemical parameters of Channa punctatus

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    Jaya Shahi

    2011-10-01

    Full Text Available Channa punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC50 of 24h of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration. The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC, mean corpuscular hemoglobin (MCH and mean corpuscular volume (MCV were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.

  1. Effect of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats.

    Science.gov (United States)

    Simeonova, Rumyana; Vitcheva, Vessela; Mitcheva, Mitka

    2010-03-01

    Tobacco smoking is a risk factor for variety of cardio-vascular diseases, such as hypertension, myocardial infarction, stroke and many others. It is of great importance for hypertensive patients to stop smoking. One of the medicines widely used for smoking cessation in Bulgaria is the original Bulgarian product Tabex®, which is developed on the basis of natural plant alkaloid cytisine. The aim of the following study was to ivestigate the effects of cytisine on some brain and hepatic biochemical parameters in spontaneously hypertensive rats (SHR), an widely used rodent model for human essential hypertension, and to compare the obtained results with their age-matched normotensive controls Wistar Kyoto (WKY). Multiple cytisine administration did not affect the activity of ethylmorphine-N-demethylase (EMND) and anylinehydroxylase (AH), as well as the quantity of cytochrome P 450, nor in WKY neither in SHR In the liver cytisine increased the MDA quantity both in SHR and in WKY, by 25% (p<0.05) and by 29% (p<0.05) respectively, while the GSH level was not significantly changed by the compound in both strains. In contrast, on the brain level, cytisine administration to SHR caused more prominent toxicity, resulted in GSH depletion and increased MDA quantity, while in WKY strain did not exert any toxicity. Cytisine did not significantly affect ALAT and ASAT activity in both strains. In conclusion, the results of our study suggest higher brain toxicity of cytisine in spontaneously hypertensive rats, that might be due to their pathophysiological characteristics.

  2. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA COLLECTED IN THE INACTIVE SEASON

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    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera in inactive season. The researches were carried out on honeybee samples collected from 5 honeybee colonies belonging to a breeding apiary of the Institute for Beekeeping Research and Development from Bucharest. In order to perform the biochemical analyses, the honeybees samples, consisting in 50 individuals on sample (10 individuals/colony were randomly collected and their haemolimph collected, at different time intervals, in inactive season (fall-winter. Totally, there were collected 250 haemolyph samples in a 2 years interval and the following 21 biochemical parameters were analysed: GLU, HDL-c, ALP, T-cho, Tprot, Alb., BUN, LDH, CPK,, Mg, IP, GGT, GOT, GPT, Ca, Cre,, Amy, T–BIL, TG, UA.. The test was carried out after the collection and processing of the samples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique, respectively .During the 2nd part of the inactive season, the values of most biochemical parameters increase in different proportions, their levels being maintained also in the first part of the active seasons (April, May, June.The values obtained for the main studied biochemical parameters in the haemolymph of the healthy honeybees collected from honeybee colonies kept in natural conditions show a highly variable evolution in the two consecutive years of experiments during the inactive season.

  3. EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS

    Science.gov (United States)

    EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS. JP Nolan1, LB Wichers2, J Stanek3, UP Kodavanti1, MCJ Schladweiler1, PA Evansky1, ER Lappi1, DL Costa1, and WP Watkinson1...

  4. Effects of cypermethrin on some clinico-hemato-biochemical and pathological parameters in male dwarf goats (Capra hircus).

    Science.gov (United States)

    Khan, Ahrar; Faridi, Hafiz A M; Ali, Muhammad; Khan, Muhammad Zargham; Siddique, Muhammad; Hussain, Iftikhar; Ahmad, Maqbool

    2009-03-01

    This study was carried out on 30 dwarf bucks to determine the effects of cypermethrin (CY) on clinical, hemato-biochemical and histopathological parameters. Animals were divided randomly into five equal groups, and each group was dipped in 0%, 0.1%, 0.4%, 0.8% or 1.6% CY, on days 0 and 15. Animals were monitored for clinical signs. Blood and serum samples were collected on day 0 and then fortnightly till day 75. Severe clinical signs comprising itching, restlessness, salivation, skin scratching and head shaking appeared at high doses (0.8% and 1.6% CY). Erythrocyte counts, hemoglobin, hematocrit, total protein, globulin and fibrinogen decreased significantly while total leukocyte counts, alanine aminotransferase and aspartate aminotransferase concentration increased significantly in all the treated groups. In the liver, necrosis of hepatocytes along with cytoplasmic vacuolation and fibroblasts proliferation were observed at a high dose of CY (1.6%). Microscopically kidneys showed congestion of parenchyma and condensation of epithelial cells of tubules along with deposition of casts in tubules. Shrinkage of glomerular capillaries and increased urinary spaces were pronounced in the high-dose group. Lungs exhibited accumulation of fibrinous exudation, thickening of alveolar walls, collapse and broken alveoli in animals treated with a high dose of CY. It was concluded that CY caused dose-dependent effects on all parameters studied. High doses of CY (0.8% and 1.6% solution) affected the parameters on erythrocytes and leukocytes for whole evaluation period, while effects on plasma proteins were transient and on ALT, AST and fibrinogen were transient but lasted a few weeks longer.

  5. Early physiological and biochemical responses of rice seedlings to low concentration of microcystin-LR.

    Science.gov (United States)

    Azevedo, Catarina C; Azevedo, Joana; Osório, Hugo; Vasconcelos, Vitor; Campos, Alexandre

    2014-03-01

    Microcystin-leucine and arginine (microcystin-LR) is a cyanotoxin produced by cyanobacteria like Microcystis aeruginosa, and it's considered a threat to water quality, agriculture, and human health. Rice (Oryza sativa) is a plant of great importance in human food consumption and economy, with extensive use around the world. It is therefore important to assess the possible effects of using water contaminated with microcystin-LR to irrigate rice crops, in order to ensure a safe, high quality product to consumers. In this study, 12 and 20-day-old plants were exposed during 2 or 7 days to a M. aeruginosa extract containing environmentally relevant microcystin-LR concentrations, 0.26-78 μg/L. Fresh and dry weight of roots and leaves, chlorophyll fluorescence, glutathione S-transferase and glutathione peroxidase activities, and protein identification by mass spectrometry through two-dimensional gel electrophoresis from root and leaf tissues, were evaluated in order to gauge the plant's physiological condition and biochemical response after toxin exposure. Results obtained from plant biomass, chlorophyll fluorescence, and enzyme activity assays showed no significant differences between control and treatment groups. However, proteomics data indicates that plants respond to M. aeruginosa extract containing environmentally relevant microcystin-LR concentrations by changing their metabolism, responding differently to different toxin concentrations. Biological processes most affected were related to protein folding and stress response, protein biosynthesis, cell signalling and gene expression regulation, and energy and carbohydrate metabolism which may denote a toxic effect induced by M. aeruginosa extract and microcystin-LR. The implications of the metabolic alterations in plant physiology and growth require further elucidation.

  6. Semen parameters and seminal plasma protein and biochemical profiles of dogs with benign prostatic hyperplasia after botulinum toxin type A intraprostatic injection

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    Tathiana Ferguson Motheo

    2014-06-01

    Full Text Available This study aimed to determine the effects of different concentrations of botulinum toxin type A (BT-A on semen parameters, and seminal plasma biochemical and protein profiles of dogs with benign prostatic hyperplasia (BPH. Eighteen sexually intact male dogs with BPH were randomly divided in three groups, and received an intraprostatic injection of saline solution (control group - CG, 250UI (GI or 500UI (GII of BT-A under transabdominal ultrasound guidance. Semen was collected at baseline, 2, 4 and 8 weeks after treatment. Semen parameters were determined and seminal plasma pH, total protein (TP, total chlorides (TC, calcium (Ca, potassium (K, and sodium (Na concentrations were assessed. One-dimensional sodium dodecyl sulfatepolyacrilamide gel eletrophoresis (SDS- PAGE was performed to determine seminal plasma protein profile. Sperm parameters and seminal plasma pH, TP, TC, Ca and K mean values did not change significantly at any time point and among treated groups (P>0.05. The SDS-PAGE analysis of the pooled fractions identified 31 protein bands with molecular weights ranging from 3.9 to 106.2kDA in all treatment groups during the entire evaluation period. Regardless the used dose, intraprostatic BT-A injection do not alter semen parameters and seminal plasma biochemical and protein profiles of dogs with BPH.

  7. Impacts of Elevated CO2 Concentration on Biochemical Composition,Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae

    Institute of Scientific and Technical Information of China (English)

    Jian-Rong XIA; Kun-Shan GAO

    2005-01-01

    To investigate the biochemical response of freshwater green algae to elevated CO2 concentrations,Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO2concentrations within the range 3-186 μmol/L and the biochemical composition, carbonic anhydrase (CA),and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate,and protein contents were enhanced to varying extents with increasing CO2 concentration from 3-186μmol/L. The CO2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa, but not in Chlamydomonas reinhardtii. The CO2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO2 concentration to 186 μmol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO2 enrichment. From this study, it can be concluded that CO2enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent.

  8. Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide.

    Science.gov (United States)

    Bownik, Adam; Stępniewska, Zofia

    2015-04-01

    Ectoine (ECT) is an osmoprotectant produced by halophilic microorganisms inducing protective effects against various stressful factors. However, little is known about its influence on aquatic invertebrates subjected to hydrogen peroxide (H2O2)-a commonly used oxidative disinfectant. Therefore, the aim of our study was to determine the effects of H2O2 alone (at 5 and 10 mg/L) and in the combination with various concentrations of ECT (5, 10 and 25 mg/L) on behavioral, physiological and biochemical parameters of Daphnia magna. The following endpoints were determined: mortality, heart rate, thoracic limb movement, total glutathione (GSH)/oxidized glutathione (GSSG) ratio, catalase (CAT) activity and nitric oxide (NOx) level. The study showed that daphnids exposed to the combination of H2O2+ECT showed decreased mortality, attenuated inhibition of heart rate and thoracic limb activity, less decreased GSH/GSSG ratio, lower stimulation of CAT activity and NOx level when compared to the crustaceans exposed to H2O2 alone. The most pronounced alleviation of toxic effects was observed in the combination of 5 mg/L H2O2+25 mg/L ECT. The results suggest that protective effects of ECT in D. magna subjected to H2O2 may be related to antioxidative properties of the osmoprotectant.

  9. Influence of magnesium on biochemical parameters of iron and oxidative stress in patients with type 2 diabetes

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    Fabiane Araùjo Sampaio

    Full Text Available Introduction: Studies have shown that oxidative stress, found in patients with type 2 diabetes, may be due to changes in the metabolism of minerals, such as magnesium and iron. Data related to compartmentalization of these minerals in diabetes are scarce and controversial. Objective: This study assessed the influence of magnesium on biochemical parameters of iron and oxidative stress in patients with type 2 diabetes. Methods: A case-control study in male and female subjects aged 27-59 years, divided into two groups: type 2 diabetes (n=40 and control (n=48. Intake of magnesium and iron was assessed by three-day food record. Plasma, erythrocyte and urinary levels of magnesium, serum iron, ferritin, total iron binding capacity, fasting glucose, glycated hemoglobin, insulin, creatinine clearance and plasma thiobarbituric acid reactive substances (TBARS were analyzed. Results and Discussion: Magnesium intake and plasma magnesium were lower in diabetic subjects. There was low urinary magnesium excretion, with no difference between groups. Although normal, the diabetic group had lower serum iron and ferritin concentrations compared to control subjects. Plasma TBARS in diabetic patients was higher than control while creatinine clearance was lower. An inverse correlation between erythrocyte magnesium and serum iron and ferritin was observed in the diabetes group. Conclusions: Diabetes induced hypomagnesemia and this, associated with chronic hyperglycemia, may have enhanced oxidative stress. Erythrocyte magnesium may have contributed to prevent iron overload and worsening of oxidative stress and hyperglycemic status.

  10. Effect of Different Growth Conditions on Certain Biochemical Parameters of Different Cyanobacterial Strains

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    Hammouda, O. E.

    2012-01-01

    Full Text Available Aims: Variation in the traditional growth medium conditions to enhance the production of lipids, carbohydrates, protein and the free amino acids content of three cyanobacterial species. Methodology and Results: Three species of cyanobacteria (Anabaena laxa, Anabaena fertilissima and Nostoc muscorum were collected from the culture collection of Soils, Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt, to investigate their biochemical composition under different growth conditions, using BG110 (nitrogen free as growth medium. These conditions were represented by control medium, static glucose medium with (1%, w/v, aerated medium (aerated by bubbling technique depending on CO2 normally existed in air with a concentration of 0.03%, molasses medium (0.7%, v/v and aerated medium enriched with glucose (1%, w/v. Lipid content, total carbohydrates, soluble proteins and free amino acids were determined at the previous conditions. Glucose at 0.7% (w/v was the most favorable for lipid production in A. laxa, where it exhibited the highest lipid content (427 μg/g fresh wt.. Increasing molasses concentration up to 0.7% (v/v produced an increase in lipid contents of the tested cyanobacterial strains. The highest lipid content of both N. muscorum (366.2 μg/g fresh wt. and A. laxa (357.4 μg/g fresh wt. were recorded at molasses concentrations of 0.1 and 0.7% (v/v, respectively. A. laxa expressed high significant values for both proteins (31.6 μg/mL and free amino acids (40.5 mg/g dry wt. after 6 days of incubation period under aerated enriched glucose condition (1%, w/v. Also, at the same growth conditions, A. fertilissima exhibited high significant values for carbohydrates at 4th day (876.8 mg/g dry wt.. Conclusion, significance and impact of study: Aerated enriched glucose medium (1%, w/v was the best growth medium condition used in the present study.

  11. Effect Of Some Herbal Medicine On Some biochemical parameters In Diabetic Rats

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    Eman G.E. Helal, Mervat A.Abbas

    2006-03-01

    Full Text Available The current study was planned to follow up the effect of famous mixture used as a hypoglycemic traditional medicine in Saudi Arabia on diabetic albino rats and their effects on some biochemical parameter as DHEA .This study also aimed to study the effect of each plants alone to illustrate the most powerful one of them. The present study was carried out on eighty adult male albino rats (120+20gm b.wt. They were randomly divided into eight groups. First group conserved as control group.The others groups received alloxan to become diabetic .The second group conserved as diabetic, the third group treated with a mixture of plants (0.5gm/120gm b.wt.From he fourth to eight group,the rats treated with Nigella sativa (0.25gm/120gm b.wt,Aloe vera,Ferulaassa-foetid,Boswellia Carterii Birdw and Commiphora myrrha respectively (0.5gm/120gm b.wt..Our result showed that the body weight was reduced significantly in diabetic group and Nigella sativa treated group. On the other hand, treatment with commiphora myrrha revealed significant increase in body weight gain, while mixture and Aloe vera treated rats recorded insignificant change. Diabetic rats revealed a significant decrease in serum glucose and DHEA-S levels and liver glycogen content, while insignificant difference was recoded in all treated groups. A significant decrease in serum insulin level was observed in diabetic group, Nigella sativa and Ferula assa-foetida treated groups, but insignificant different was recorded in the rest treated groups throughout the experiment. A significant increase in LDH activities in diabetic and Ferula assa-foetida treated groups was recorded while rest treated groups recorded insignificant change During experimental period.

  12. Lymphocytic Thyroiditis - is cytological grading significant? A correlation of grades with clinical, biochemical, ultrasonographic and radionuclide parameters

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    Bhatia Alka

    2007-01-01

    Full Text Available Background: Clinical, biochemical, ultrasonographic, radionuclide and cytomorphological observations in Lymphocytic thyroiditis (LT, to define the cytological grading criteria on smears and correlation of grades with above parameters. Methods: This prospective study was conducted on 76 patients attending the Fine needle aspiration cytology clinic of a tertiary care institute in North India. The various parameters like patients′ clinical presentation, thyroid antimicrosomal antibodies, hormonal profiles, radionuclide thyroid scan and thyroid ultrasound were studied. Fine needle aspiration of thyroid gland and grading of thyroiditis was done on smears. The grades were correlated with above parameters and the correlation indices were evaluated statistically. Results: Most of the patients were females (70, 92.11% who presented with a diffuse goiter (68, 89.47%. Hypothyroid features (56, 73.68% and elevated TSH (75, 98.68% were common, but radioiodide uptake was low or normal in majority of patients. Thyroid antimicrosomal antibody was elevated in 46/70 (65.71% patients. Cytomorphology in fine needle aspirates was diagnostic of lymphocytic thyroiditis in 75 (98.68% patients. Most of them had grade I/II disease by cytology. No correlation was observed between grades of cytomorphology and clinical, biochemical, ultrasonographic and radionuclide parameters. Conclusion: Despite the availability of several tests for diagnosis of LT, FNAC remains the gold standard. The grades of thyroiditis at cytology however do not correlate with clinical, biochemical, radionuclide and ultrasonographic parameters.

  13. Lymphocytic Thyroiditis – is cytological grading significant? A correlation of grades with clinical, biochemical, ltrasonographic and radionuclide parameters

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    Dash Radharaman

    2007-01-01

    Full Text Available Abstract Background Clinical, biochemical, ultrasonographic, radionuclide and cytomorphological observations in Lymphocytic thyroiditis (LT, to define the cytological grading criteria on smears and correlation of grades with above parameters. Methods This prospective study was conducted on 76 patients attending the Fine needle aspiration cytology clinic of a tertiary care institute in North India. The various parameters like patients' clinical presentation, thyroid antimicrosomal antibodies, hormonal profiles, radionuclide thyroid scan and thyroid ultrasound were studied. Fine needle aspiration of thyroid gland and grading of thyroiditis was done on smears. The grades were correlated with above parameters and the correlation indices were evaluated statistically. Results Most of the patients were females (70, 92.11% who presented with a diffuse goiter (68, 89.47%. Hypothyroid features (56, 73.68% and elevated TSH (75, 98.68% were common, but radioiodide uptake was low or normal in majority of patients. Thyroid antimicrosomal antibody was elevated in 46/70 (65.71% patients. Cytomorphology in fine needle aspirates was diagnostic of lymphocytic thyroiditis in 75 (98.68% patients. Most of them had grade I/II disease by cytology. No correlation was observed between grades of cytomorphology and clinical, biochemical, ultrasonographic and radionuclide parameters. Conclusion Despite the availability of several tests for diagnosis of LT, FNAC remains the gold standard. The grades of thyroiditis at cytology however do not correlate with clinical, biochemical, radionuclide and ultrasonographic parameters.

  14. Endocrinological, biophysical, and biochemical parameters of semen collected via masturbation versus sexual intercourse.

    Science.gov (United States)

    Sofikitis, N V; Miyagawa, I

    1993-01-01

    In clinical programs of assisted reproduction involving infertile males, it is essential to obtain semen of maximum quality. To evaluate ways of achieving this objective, and to assess the fertilizing capacity of the sperm, six semen samples were collected from each of 38 infertile men via masturbation. Six more samples were then collected from each man at sexual intercourse using a semen collection device (SCD). We confirmed that the volume of seminal plasma, total sperm count, sperm motility, and percentage of morphologically normal spermatozoa were significantly higher in samples collected at intercourse than masturbation, as reported previously. In addition, the markers of the secretory function of the prostate and the outcome of sperm function tests (hypoosmotic swelling test, acrosin assay, and sperm penetration assay) were significantly higher for the samples collected at intercourse. There were no significant differences in markers of the secretory function of the seminal vesicles and epididymis between the samples. The improved spermatozoal parameters in the samples collected at intercourse may reflect a higher prostatic secretory function at that time. There were no significant differences in the serum concentrations of gonadotropins, or in the serum or seminal plasma concentrations of testosterone, before or after masturbation or sexual intercourse. Therefore, the differences in prostatic secretory function and semen parameters may not be attributed to differences in hormonal levels. Semen collection during intercourse using an SCD appears to be the method of choice for selecting semen samples for artificial insemination.

  15. Effects of dietary chromium picolinate and peppermint essential oil on growth performance and blood biochemical parameters of broiler chicks reared under heat stress conditions

    Science.gov (United States)

    Akbari, Mohsen; Torki, Mehran

    2014-08-01

    A study was conducted using 240 female day-old broiler chicks to evaluate the effects of dietary chromium picolinate (CrPic), peppermint essential oil (P.mint), or their combination on growth performance and blood biochemical parameters of female broiler chicks raised under heat stress conditions (HS, 23.9 to 38 °C cycling). Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) were obtained from 1 to 42 days of age. Furthermore, at the end of the experiment (day 42), birds were bled to determine some blood biochemical parameters and weighed for final body weight (BW). ADFI, ADG, and BW were not influenced significantly by dietary CrPic and P.mint ( P > 0.05). A significant interaction between dietary CrPic and P.mint on FCR ( P = 0.012) was detected. FCR significantly decreased in chicks fed the diet including both CrPic and P.mint compared with the CrPic group. Significant interaction between dietary P.mint and CrPic on serum concentrations of triglycerides, glucose, and albumin were observed ( P 0.05). The serum concentrations of glucose, triglycerides were decreased ( P diet including both CrPic and P.mint. Plasma chromium (Cr) content increased significantly ( P diet compared with the control group ( P < 0.05). From the results of the present experiment it can be concluded that dietary supplementation with combined P.mint and CrPic could have beneficial effects on some blood biochemical parameters of female chicks reared under heat stress conditions.

  16. Investigation on some biochemical parameters and effect of hormonal treatment in anoestrous dairy cows with cystic ovarian follicle

    Institute of Scientific and Technical Information of China (English)

    StanimirA Yotov; AnatoliS Atanasov; GeorgiB Georgiev; JulietaD Dineva; NadejdaA Palova

    2014-01-01

    Objective:To investigate some blood biochemical parameters in anoestrous dairy cows with cystic ovarian follicles(COF) during the first two months post partum and the effect of hCG-PGF2α-GnRH treatment on their reproductive response.Methods:The investigation was performed on20 lactatingHolstein cows, divided into two groups: control group(n=8) and treated group (n=12).All animals had cystic ovarian follicles, detected during the two consecutive transrectal ultrasound examinations at7-day interval.Blood samples from each animal were collected on the day of second ultrasonography(Day0) and subjected to a hormonal and biochemical analysis. Treated cows were subjected to another ultrasound examination on day of prostaglandin injection and the presence of corpus luteum was recorded.The control animals were not treated, while the treated group received1500IU hCG on day0,PGF2α500 mg on day7 and100 μgGnRH 48 hours latter.Eighteen hours afterGnRH administration animals bearing corpus luteum on Day7 were artificiallly inseminated.The last ultrasound examination was made forty days after start of the threapy.On the base of the obtained results, cumulative oestrous activity, cumulative ovulation rate, pregnancy rate andCOF persistence were determined.Results:The blood analysis in the both groups onDay0 showed significantly(P<0.05) lower mean concentration of progesterone, glucose and inorganic phosphate compared to the reference range values for cyclic dairy cows.Cumulative oestrous activity(91.7%) and cumulative ovulation rate(75.0%) were considerably higher(P<0.05) in treated group than control group, whereas the cystic follicle persistence(25.0%) was lower(P<0.05) in the treated group than control group.Conclusions:The present study confirmed the thesis for low blood levels of glucose and inorganic phosphate in cows presenting cystic ovarian follicles.It also indicated significant improvement of cumulative oestrous activity and ovulation rate in animals subjected toh

  17. Comparative Evaluation of Physiological and Biochemical Parameters and Survival of Chickens for the Effects of Microclimatic Stress Factors During Incubation

    OpenAIRE

    Indyuhova E.N.

    2015-01-01

    The article presents the results of two experiments, the findings reflect the influence of temperature and moisture stress on physiological and biochemical parameters of chickens and their viability during embryonic and early postembryonic development. It was found that under the influence of stress factors recorded increase in lipid peroxidation in chickens from day-old experimental groups; showed a reduction in the intensity of the major metabolism of young chickens day old.

  18. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

    OpenAIRE

    Ye. Barylo

    2016-01-01

    Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae) of brown trout, rainbow trout and brook trout we used as study mat...

  19. Effects of 24 h ultra-marathon on biochemical and hematological parameters

    Institute of Scientific and Technical Information of China (English)

    Huey-June Wu; Kung-Tung Chen; Bing-Wu Shee; Huan-Cheng Chang; Yi-Jen Huang; Rong-Sen Yang

    2004-01-01

    AIM: To analyze detailed changes in hematology and biochemistry tests parameters before and after a longdistance race in ultramarathon runners. METHODS: Blood samples of 11 participants were obtained for standard analysis before, immediately after, two days after and nine days after the 2002 International Ultramarathon 24 h Race and the International Association of Ultrarunners (IAU) Asia 24 h Championship. RESULTS: Total bilirubin (BIL-T), direct bilirubin (BIL-D), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased statistically significantly (P<0.05) the race. Significant declines (P<0.05) in red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) were detected two days and nine days d after the race. 2 d after the race, total protein (TP), concentration of albumin and globulin decreased significantly. While BIL, BIL-D and ALP recovered to their original levels. High-density lipoprotein cholesterol (HDL-C) remained unchanged immediately after the race, but it was significantly decreased on the second and ninth days after the race.CONCLUSION: Ultra-marathon running is associated with a wide range of significant changes in hematological parameters, several of which are injury related. To provide appropriate health care and intervention, the man who receives athletes on high frequent training program high intensity training programs must monitor their liver and gallbladder function.

  20. Antioxidant status and selected biochemical parameters of porcine ovarian granulosa cells exposed to lead in vitro.

    Science.gov (United States)

    Capcarová, Marcela; Kolesárová, Adriana; Lukác, Norbert; Sirotkin, Alexander; Roychoudhury, Shubhadeep

    2009-12-01

    The objective of this study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) and release of calcium, phosphorus, magnesium, sodium, potassium, total lipids, totals proteins, glucose, cholesterol and triglycerides by porcine ovarian granulosa cells cultured in vitro after lead acetate administration. The parameters were analyzed using semi-automated clinical chemistry analyzer Microlab 300, microprocessor-controlled analyzer EasyLite and spectrophotometer Genesys 10. Cells were cultured with lead acetate trihydrate [Pb(CH(3)COO)(2).3H(2)O] as follows: group Max (5 mg Pb(CH(3)COO)(2).3H(2)O/10 mL), group A (2.5 mg/10 mL), group B (0.83 mg/10 mL), group C (0.625 mg/10 mL), group D (0.455 mg/10 mL) and the control group without lead exposure for 18 hrs. The highest TAS was estimated in the control group without lead treatment in comparison with other groups (MAX, A, B, C, D). Statistical analyses showed significantly lower value (P 0.05) were detected in concentration of other studied parameters among observed groups, too.

  1. Quantification of extraprostatic extension in prostate cancer: different parameters correlated to biochemical recurrence after radical prostatectomy

    NARCIS (Netherlands)

    Veggel, B.A. van; Oort, I.M. van; Witjes, J.A.; Kiemeney, L.A.L.M.; Hulsbergen- van de Kaa, C.A.

    2011-01-01

    AIMS: Different methods to substage extraprostatic extension (EPE) were correlated with biochemical recurrence (BCR) after radical prostatectomy (RP). Methods and results: A total of 157 consecutive RP specimens with EPE were completely embedded. Twenty-three patients with adjuvant therapy or detect

  2. Biochemical parameters of bone metabolism in bone metastases of solid tumors (Review)

    NARCIS (Netherlands)

    Meijer, Wilhelmus; van der Veer, E; Willemse, P H

    1998-01-01

    The role of biochemical markers of bone metabolism in the diagnosis and monitoring of bone metastases in solid tumors is reviewed. Emphasis is on the recently developed markers, which may provide a more accurate quantitation of bone metabolism. In metastatic bone disease, bone formation and resorpti

  3. Bioaccumulation and oxidative stress parameters in silver catfish (Rhamdia quelen) exposed to different thorium concentrations.

    Science.gov (United States)

    Kochhann, Daiani; Pavanato, Maria A; Llesuy, Susana F; Correa, Lizelia M; Konzen Riffel, Ana P; Loro, Vania L; Mesko, Márcia F; Flores, Erico M M; Dressler, Valderi L; Baldisserotto, Bernardo

    2009-10-01

    The objective of this study was to evaluate the effect of chronic thorium (Th) exposure on bioaccumulation, metabolism (through biochemical parameters of the muscle) and oxidative parameters (lipidic peroxidation levels and antioxidant enzymes in the gills and in the hepatic and muscular tissues) of silver catfish (Rhamdia quelen). Silver catfish juveniles were exposed to different waterborne Th levels (in microg L(-1)): 0 (control), 25.3+/-3.2, 80.6+/-12.0, 242.4+/-35.6, and 747.2+/-59.1 for 30 d. The gills and skin were the organs that accumulated the highest Th levels. The increase in the waterborne Th concentration corresponded to a progressive increase in the Th levels in the gills and kidney. Chronic Th exposure causes alterations in the oxidative parameters of silver catfish gills, which are correlated with the Th accumulation in this organ. The levels of GST decreased in the gills of fish exposed to 747.2 microg L(-1) Th and SOD activity decreased in silver catfish exposed to 242.4 and 747.2 microg L(-1) Th. In addition, the increase in the LPO in the gills exposed to 242.4 and 747.2 microg L(-1) Th suggests that higher oxidative damage occurred in the gills. However, in the liver and muscle, these alterations occurred mainly in the lowest waterborne Th level. Metabolic intermediates in the muscle were altered by Th exposure, but no clear relationship was found.

  4. On-line estimation of concentration parameters in fermentation processes

    Institute of Scientific and Technical Information of China (English)

    XIONG Zhi-hua; HUANG Guo-hong; SHAO Hui-he

    2005-01-01

    It has long been thought that bioprocess, with their inherent measurement difficulties and complex dynamics, posed almost insurmountable problems to engineers. A novel software sensor is proposed to make more effective use of those measurements that are already available, which enable improvement in fermentation process control. The proposed method is based on mixtures of Gaussian processes (GP) with expectation maximization (EM) algorithm employed for parameter estimation of mixture of models. The mixture model can alleviate computational complexity of GP and also accord with changes of operating condition in fermentation processes, i.e., it would certainly be able to examine what types of process-knowledge would be most relevant for local models' specific operating points of the process and then combine them into a global one. Demonstrated by on-line estimate of yeast concentration in fermentation industry as an example, it is shown that soft sensor based state estimation is a powerful technique for both enhancing automatic control performance of biological systems and implementing on-line monitoring and optimization.

  5. Effects Of Music Therapy On Clinical And Biochemical Parameters Of Metabolic Syndrome

    Directory of Open Access Journals (Sweden)

    Rajnee

    2011-12-01

    Full Text Available Background: Music therapy is a new approach being used for the management of metabolic abnormalities and stress related illness.Objective: To study the effect of Music therapy on various clinical and biochemical parameters of Metabolic Syndrome.Methods: This cross sectional study was carried out on 100 patients of metabolic syndrome selected randomly. These patients were divided into two equal groups after age, sex adjustment. In control group (group I 50 patients underwent the conventional treatment. 50 patients in study group were treated with supervised music protocol along with conventional treatment. The Body Mass Index, ;Waist-Hip ratio, Blood pressure, Fasting blood sugar were monitored weekly while HbA1c and lipid profile were determined at the baseline and after three months of exposure to music therapy. Statistical analysis was performed by employing student t- test.Results: In the study group there was a significant decrease in BMI (27.18±5.02 to 25.44±3.49 kg/m2, p<0.05, waist hip ratio (0.95±0.05 to 0.93±0.05 cm, p<0.05, Fasting blood sugar (196.00±47.80mg/ dl to152.00±16.19mg/dl , p<0.001, HbA1c (8.41±1.31% to 7.08±0.78 % p<0.001, Systolic Blood Pressure (151.00±12.10 to 136±9.04 mmHg p<0.001, Diastolic Blood Pressure (94±4.80 to 86.44±3.16 mmHg, p<0.01, Mean serum cholesterol (257.80±18.92 to 229.12±17.82mg/dl, p<0.001 and triglycerides (180.86±14.04 to 136.50±8.92mg/dl, p<0.001, LDL (167.97±14.40 to 140.20±15.41mg/dl, p<0.001, and VLDL (33.60±2.88 to 28.04±3.08mg/dl, p<0.001 and increase in HDL (33.32±3.38 to 39.71±3.41mg/dl, p<0.001, when compared with those of control group not receiving the music therapy along with the conventional treatment.Conclusion: The promising outcomes of Music therapy showed that it may be considered as a useful adjunct to conventional treatment in management of the metabolic syndrome. This study advocates music therapy to establish it from a general well being concepts to a

  6. Haematological and biochemical blood parameters in horses used for sport and recreation

    OpenAIRE

    2015-01-01

    The study was conducted to determine and compare the resting values of haematological and biochemical blood indices in clinically healthy horses competing in show jumping and used for leisure activities. The experiment included twenty adult horses of Polish half-bred andWielkopolska breed which were subjected to a daily training programme typical of their type of use. Blood samples were collected at rest before feeding from the jugular external vein. Show jumpers had higher (P

  7. Study of Some Hematological and Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) Fry

    OpenAIRE

    Salman Rauoof CHALKOO; Imtiyaz Ahmed SHEIKH; Lone, Ghulam Nabi; Shaukath Ali MIR

    2013-01-01

    This study was done to investigate of some hematological and biochemical factors of rainbow trout (Oncorhynchus mykiss) fry in Kashmir. About 50 pools of blood samples from diseased fry were collected within 30 months from November 2008 till March 2011 from three hatchery farms. In addition 30 pools of blood samples as control group were collected randomly from same farms. Each blood samples were examined for whole blood examination and blood enzymes measurement. It consist of total leukocyte...

  8. Subclinical hypocalcemia, plasma biochemical parameters, lipid metabolism, postpartum disease, and fertility in postparturient dairy cows.

    Science.gov (United States)

    Chamberlin, W G; Middleton, J R; Spain, J N; Johnson, G C; Ellersieck, M R; Pithua, P

    2013-01-01

    A study was conducted to evaluate the potential association between Ca status at calving and postpartum energy balance, liver lipid infiltration, disease occurrence, milk yield and quality parameters, and fertility in Holstein cows. One hundred cows were assigned to 1 of 2 groups based on whole-blood ionized Ca concentration ([iCa]) on the day of calving [d 0; hypocalcemic [iCa] Cows were blocked based on calving date and parity. Blood samples were collected approximately 14 d from expected calving date (d -14), the day of calving (d 0), and on d 3, 7, 14, 21, and 35 postpartum for measurement of plasma nonesterified fatty acid, iCa, total Ca, glucose, and total and direct bilirubin concentrations, and plasma aspartate aminotransferase and gamma glutamyl transferase activities. Liver biopsies were obtained from a subset of cows on d 0, 7, and 35 for quantification of lipid content. Milk samples were collected on d 3, 7, 14, 21, and 35 postpartum for measurement of somatic cell count and percentages of protein, fat, and solids-not-fat. Data for peak test-day milk yield, services per conception, and days open were obtained from Dairy Herd Improvement Association herd records. Disease occurrence was determined based on herd treatment records. Hypocalcemic cows had significantly higher nonesterified fatty acids on d 0. Hypocalcemic cows also had significantly more lipid in hepatocytes on d 7 and 35 postpartum. However, no statistically significant differences were observed between groups for plasma aspartate aminotransferase and gamma glutamyl transferase activities or total and direct bilirubin concentrations. Milk protein percentage was lower in hypocalcemic cows on d 21 and 35. However other milk quality variables (somatic cell count, milk fat percentage, and solids-not-fat) and milk yield variables (peak test-day milk yield and 305-d mature-equivalent 4% fat-corrected milk yield) did not differ between groups. No differences were observed between groups in the

  9. Alteration of biochemical parameters after supplementation of multivitamins and minerals of sprayers on grape gardens of Western Maharashtra (India

    Directory of Open Access Journals (Sweden)

    Jyotsna A. Patil

    2014-01-01

    Full Text Available Background: In the horticulture sector, the land used for cash crop like grapes is increasing particularly in Maharashtra state. There is an increasing use of the pesticides in an attempt to increase the yield and reduce the post harvest losses. The environmental pollution and poisoning due to wide use of pesticide during grape cultivation may be much more important factors to disturb the socio economical status of uneducated farm worker in rural areas. Aim and Objectives: The study aimed to evaluate the pesticide exposure of the population associated with grape cultivation with respect to biochemical effects, oxidative stress, antioxidants status and protecting them from such effects by supplementing vitamins A to Z. Material and Methods: For this study 30 subjects with occupational pesticides exposure i.e. sprayers of grape gardens having age in the range of 20 to 45 years from Tasgaon taluka, Sangli district, (Western Maharashtra India were taken and all these study group subject,500 mg vitamin A to Z tablet / day for fifteen days were supplemented. The blood was collected by venipuncture from all these subjects for biochemical parameters assay before and after fifteen days of vitamins A to Z supplementation and all biochemical parameters were estimated by using standard methods. Results: Fifteen days vitamin A to Z and multi minerals supplementation to sprayers of grape gardens, we observed significant increase in serum Acetyl Cholinesterase (P<0.001, 2.32%, and decreased Aspartate Transaminase (P<0.05, 8.36%, Alanine Transaminase (P<0.05, 14.16%, serum Lipid Peroxide (P<0.001, 24.39%, Glutathione S-Transferase (P<0.01, 29.84% and increased RBC-Superoxide Dismutase (P<0.05, 11.55%, RBC-Catalase (P<0.001, 25.7%, serum zinc (P<0.01, 3.74%, copper (P<0.001, 4.13%, blood lead (P<0.01, 3.33% as compared to before vitamin A to Z and multi minerals supplementation and other biochemical parameters such as serum C-Reactive Proteins, Total Proteins

  10. Coconut oil on biochemical and morphological parameters in rats submitted to normolipidic and hyperlipidic diets

    Directory of Open Access Journals (Sweden)

    Bianca de Oliveira Schumacher

    Full Text Available ABSTRACT: This study aimed to evaluate the influence of replacing soybean oil with extra virgin coconut oil in normolipidic and hyperlipidic diets, on the lipid metabolism of Wistar rats. In the first stage of the experiment (30 days, 36 rats were divided into 2 groups and fed with a control or a hyperlipidic diet. Six animals from each group were then killed, and the remaining rats were redistributed into 4 new groups: 2 groups remained on the control and hyperlipidic diets, and in the diets of the other 2 groups, the soybean oil was replaced with coconut oil (30 days. At the end of the assay, the biological models were decapitated for blood collection and removal of organs and peritoneal fat. Although the diet intake differed among groups during both stages of the experiment, no differences were noted with regard to weight gain and peritoneal fat. Replacing soybean oil with coconut oil in the rat diet lowered triglyceride and low-density lipoprotein serum concentrations in both groups. Liver parameters, namely, total cholesterol and triacylglycerols, increased with the substitution of soybean oil by coconut oil in the normolipidic diet and decreased in the hyperlipidic diet. Thus, replacing soybean oil by coconut oil may improve serum and liver lipid levels in Wistar rats.

  11. THE EFFECT OF THE KETOGENIC DIET ON THE GROWTH AND BIOCHEMICAL PARAMETERS OF THE CHILDREN WITH RESISTANT EPILEPSY

    Directory of Open Access Journals (Sweden)

    Mohammadreza ALAEI

    2010-04-01

    Full Text Available ObjectiveThe aim of this study was to evaluate the effect of the ketogenic diet on the growth parameters of the children with resistant epilepsy.Materials & MethodsA total of 36 children with resistant epilepsy who were 2 to 7 year old were put on the ketogenic diet. Their growth and biochemical parameters were studied at the beginning of the study and after 3 months.ResultsWeight decreased in all patients. Serum levels of hemoglobin, calcium, and blood sugar decreased significantly but remained in the normal range. Creatinine did not change, but BUN showed a significant increase.ConclusionWe can lower the complications of ketogenic diets by using more unsaturated fat, more water, and more minerals.Keywords: ketogenic diet, epilepsy, growth parameters, biochemicalparameters, children

  12. Effect of imatinib on the biochemical parameters of the reproductive function in male Swiss albino mice

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    A M Prasad

    2011-01-01

    Full Text Available Background: Treatment of cancers with cytotoxic agents such as tyrosine kinase inhibiting drugs often, but not always, result in transient to permanent testicular dysfunction. Germ cells are important targets of many chemicals. Most of the drugs are genotoxins and induce irreversible effect on genetic makeup. These mutagenic changes are proportionally related to carcinogenesis. This is alarmingly dangerous in youth and children, since these effects last longer, affecting fertility or forming basis for carcinogenesis. There is paucity of reports on planned studies of imatinib on the testicular function. Hence, the study was planned to assess the effects of imatinib on biochemical markers of testicular functions in male Swiss albino mice. Materials and Methods: Male Swiss albino mice were treated with imatinib and sacrificed at the end of first, second, fourth, fifth, seventh, and tenth week after the last exposure to imatinib. The testis were removed, weighed, and processed for biochemical analysis. Results: The intratesticular testosterone level was significantly (P<0.001 reduced in treated groups and severe effect was observed on week 4 and 5. The intratesticular lactate dehydrogenase (LDH level was significantly increased by imatinib in all treated groups up to week 5. Conclusion: Imatinib does affect testosterone and LDH level significantly, but this effect is reversible once the drug is withdrawn. This finding may help the clinicians to plan and address the fertility-related issues in young patients of reproductive age who are being treated with imatinib for gastrointestinal tumors and chronic myeloid leukemia.

  13. THE VARIATION OF SOME BIOCHEMICAL PARAMETERS FROM FRESH COW CHEESE CREAM TYPE WITH ADDED AROMATIC PLANTS

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    CRISTIANA DIACONESCU

    2013-12-01

    Full Text Available Recent advanced studies in the field of cheese fabrication technology include, amongother, the usage of aromatic plants and spices as a mean of improving theorganoleptic properties and as a mean of decreasing the lipidic peroxidationphenomenon. For this purpose, three types of fresh cow cheese produced by S.C.Marion Invest Trade 94 SRL, experimentally added with dill, savory and rosemary,were comparatively studied from a chemical point of view (pH, acidity, humidity, ashand a biochemical point of view(raw fat, total proteins, total cholesterol, acidphosphatase’s enzymatic activity, superoxide dismutase’s enzymatic activity, calciumcontent.From a chemical point of view, no significant differences appeared between the creamcheese types analyzed, excepting the pH( lower in the cheeses with dill and rosemaryFrom a biochemical point of view, two phenomenon can be observed: a significantdecrease of the acidic phosphatase activity in the cheeses with added dill androsemary ( positively correlating with the pH value and decreased values of the SODactivity in all cheese types.

  14. Effects of dietary administration of yarrow extract on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss

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    Mahmoud Nafisi Bahabadi

    2014-10-01

    Full Text Available The present study was conducted to investigate the clinical effects and possible side effects of yarrow extract (Achillea millefolium L. as feed additive on biochemical blood parameters and growth performance of rainbow trout (Oncorhynchus mykiss. Fishes were treated with 0 (control, 0.1, 0.5 and 1% of yarrow extract for 30 days. Plasma alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, alkaline phosphatase (ALP, creatine kinase (CK, peroxidase activity, total complement and lysozyme activity, glucose, total protein, triglyceride and cholesterol were measured after 15 and 30 days of yarrow treatment. There were no significant changes in the lysozyme activity and glucose levels. Total protein and globulin levels were significantly higher in the fish fed with diets enriched with 1% yarrow extract on day 30. Triglyceride and cholesterol levels was significantly decreased in the fish fed with diets containing 0.5% and 1% yarrow extract on day 30 (P<0.05. LDH, CK and peroxidase activities in the fish fed with diets having 1% yarrow extract were significantly decreased at the end of the experiment (P<0.05. In contrast, a significant increase in AST, ALP and total complement activity was observed in the fish fed with 1% yarrow extract diet, on day 15 (P<0.05. The weight gain and specific growth rate increased and food conversion ratio decreased in in the fish fed 1% yarrow extract on day 30. Condition factor in the fish fed with yarrow extract was significantly higher than control group on 30 day. In conclusion, on the basis of these results, oral administration of yarrow extract up to 0.5% have not side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 1% of yarrow extract caused cytotoxicity and modifications in blood biochemical parameters of fish.

  15. Correlation between Aminotransferase Ratio (AST/ALT and Other Biochemical Parameters in Chronic Liver Disease of Viral Origin

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    Shah Md Fazlul Karim

    2015-03-01

    Full Text Available Background: In recent years the ratio of aspartate aminotransferase (AST to alanine aminotransferase (ALT in patients of chronic liver disease (CLD of various origins has gained much attention. This variable is readily available, easy to interpret, and inexpensive and the clinical utility of the AST/ALT ratio in the diagnostic workup of patients with CLD is quite promising. Objective: The present study was designed to find out the link between aminotransferase (AST/ALT ratio with commonly measured biochemical parameters of liver function tests in CLD of viral origin. Materials and method: This cross sectional study was carried out in the department of Biochemistry, Sir Salimullah Medical College, Dhaka, Bangladesh. Forty four biopsy proven diagnosed subjects of chronic viral hepatitis without cirrhosis of both sex were selected purposively. With aseptic precaution 5 mL venous blood was collected from each subject and common liver function tests (serum AST, ALT, AST/ALT ratio, alkaline phosphatase, total bilirubin, serum total protein, serum albumin, serum globulin, serum albumin/globulin ratio, prothrombin time and viral serology (HBsAg, Anti HDV antibody, Anti HCV antibody were performed. Data were analyzed by SPSS version 19 for Windows. Pearson’s correlation test was done to determine association between AST/ALT with other biochemical parameters. Results: Mean(±SD age of the study subjects was 32.55±10.55 years (range 20-50 years with 48 (77.7% male and 14 (22.6% female subjects. Pearson’s correlation test was done between AST to ALT ratio with other biochemical parameters and prothrombin time showed significant positive correlation (p <0.01. Conclusion: In our study we found significant positive correlation between AST/ALT with prothrombin time in CLD subjects without cirrhosis.

  16. STUDY OF CLINICAL AND BIOCHEMICAL PARAMETERS IN PREDICTING THE NEED FOR VENTILATOR SUPPORT IN ORGANOPHOSPHORUS COMPOUND POISONING

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    Rajeev

    2013-12-01

    Full Text Available Organophosphorus compound is used for committing suicidal are on upswing in developing countries A grading system of severity of OP poisoning suggests that most cases can be managed in the ICU which cannot be applied to developing countries, where facilities for ICU management are rather limited. Hence, the pr esent study is undertaken to identify the factors both clinical and biochemical, which help in predicting the need for ventilator support and thus helping to reduce the mortality by timely institution of ventilator support. AIMS OF THE STUDY: To study the clinical and biochemical parameters in organophosphate poisoning, which help to predict the need for ventilator support. MATERIAL AND METHODS: This is a Descriptive Study done at Kempegowda Institute of Medical Sciences, Bangalore, with a sample size of 50 cases. Patients who fulfilled the inclusion criteria are assessed as per proforma specifically designed for the study. RESULTS: In this study population, 12 patients who reached the hospital for treatment > 4 hour of consumption, 11(91.7% required ventil ator support . In this study, out of patients with pinpoint pupils at admission required ventilator support. In this study, all patients ( required ventilator support with a fasciculation score of more than as compared to none with a bsent fasciculation. In this study, lower the Glasgow coma scale at admission, more vulnerable are the patients for ventilator support . In This study, patients with reduced levels of Pseudo cholinesterase i.e. out of patients ( required ventilato r support. CONCLUSION: Clinical and biochemical parameters such as Greater the time lag from consumption of OP poison till getting specific treatment, Lower GCS scoring, Generalized Fasiculations, Low Pseudo cholinesterase levels, Larger initial dose of At ropine required for Atropinization were strong predictors for the need for Assisted Ventilation in OP poisoning. Grading of the degree of the poisoning taking

  17. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase

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    Flisser Ana

    2011-05-01

    Full Text Available Abstract Background Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP and the active labor phase (ActLP. Methods Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Results Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Conclusion Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  18. INVESTIGATION OF EFFECT OF MURRAYA KOENIGII ON BIOPHYSICAL AND BIOCHEMICAL PARAMETERS OF WOUND IN DIABETIC HYPERLIPIDEMIC WISTAR RATS

    Directory of Open Access Journals (Sweden)

    Vikram Kumar*, Angshu Bandyopadhyay and Vikram Sharma

    2012-06-01

    Full Text Available Plants have been used in traditional medicine for several thousand years. India is perhaps the largest producer of medicinal herbs and is rightly called the “Botanical garden of the World”. Murraya koenigii Linn. commonly known as Meethi neem, belongs to the family Rutaceae. The curry tree is native to India and it is found almost everywhere in the Indian subcontinent excluding the higher levels of Himalayas. The effect of Murraya koenigii leaves aqueous extract on biophysical and biochemical parameters of wound were studied by dead space wound model in diabetic hyperlipidemic rats. In dead space wound model, animals treated with Murraya koenigii (oral administration of variable dosage level 200mg/kg, 300mg/kg and 400mg/kg leaves aqueous extract showed significant increase in Wet & Dry granulations tissue weight (biophysical parameter and hydroxyprolin content (biochemical parameter when compared to the diabetic hyperlipidemic control group rats. In this study, very significant (p<0.001 result was found with 300mg/kg dose level because the effect was dose dependent up to 300mg equivalent of extract. The results suggested that aqueous extract of Murraya koenigii possess significant wound healing potential in diabetic hyperlipidemic rats. Further studies may reveal the exact mechanisms of action responsible for the wound healing activity of Murraya koenigii leaves aqueous extract in diabetic hyperlipidemic condition.

  19. Influence of drug concentration on the diffusion parameters of caffeine

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    R Ben Mustapha

    2011-01-01

    Full Text Available Background and Objectives : In the fields of the pharmaceutical and cosmetic industries and in toxicology, the study of the skin penetration of molecules is very interesting. Various studies have considered the impact of different physicochemical drug characteristics, skin thickness, and formulations, on the transition from the surface of the skin to the underlying tissues or to the systemic circulation; however, the influence of drug concentration on the permeation flux of molecules has rarely been raised. Our study aims to discover the influence of caffeine concentration in a formulation on the percutaneous penetration from gels, as a result of different dose applications to polysulfate membrane and human skin. Materials and Methods : For this purpose, three identical base gels were used at 1, 3, and 5% of caffeine, to evaluate the effect of the concentration of caffeine on in vitro release through the synthetic membrane and ex vivo permeation through the human skin, using diffusion Franz TM cells. Results : The diffusion through the epidermal tissue was significantly slower than through the synthetic membrane, which recorded an increase of flux with an increase in the concentration of caffeine. The skin permeation study showed that diffusion depended not only on the concentration, but also on the deposited amount of gel. Nevertheless, for the same amount of caffeine applied, the flux was more significant from the less concentrated gel. Conclusion : Among all the different concentrations of caffeine examined, 1% gel of caffeine applied at 5 mg / cm 2 showed the highest absorption characteristics across human skin.

  20. Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance, blood biochemical parameters, and carcass characteristics.

    Directory of Open Access Journals (Sweden)

    Zhenming Zhou

    Full Text Available Fifty-one Simmental crossbred steers (357.0 ± 16.5 kg were used to compare a standard total mix ration (TMR with variants on animal performance, ruminal fermentation, blood biochemical parameters, and carcass characteristics. Corn grain and cotton seed meal were partially replaced by ensiled mulberry leaves (EML or sun-dried mulberry fruit pomace (SMFP. Experimental diets had similar amounts of crude protein (CP, acid detergent fiber (ADF, and metabolizable energy (ME. Animals were divided into three groups: control group (CONT, 8% EML group, and 6.3% SMFP group. Performance, including average daily weight gain (ADG, and dry matter intake (DMI, was measured. Blood and rumen samples were collected at the end of the experiment (16 weeks. There were no differences in final body weight (P = 0.743, ADG (P = 0.425, DMI (P = 0.642, or ADG/DMI (P = 0.236 between the groups. There were no differences (P = 0.2024 in rumen pH values; ammonia N was lower (P = 0.0076 in SMFP than in the EML and CONT groups. There were differences in the concentrations of total and individual volatile fatty acids, while no differences were determined in blood biochemical parameters (i.e., plasma glucose, urea concentrations, triglycerides, total protein, insulin, IgG, alanine transaminase, and aspartate aminotransferase, P ≥ 0.098. No differences were observed in carcass characteristics (P ≥ 0.513, tenderness (P = 0.844, adipose and lean color values (P ≥ 0.149, and chemical composition (P ≥ 0.400; however, intramuscular fat was lower in the EML and SMFP groups compared to the CONT animals (P = 0.034. In conclusion, diets supplemented with these two mulberry products in an isocaloric and isonitrogenous manner have similar effects to corn grain and cotton seed meals on steer performance, blood biochemical parameters and carcass characteristics, with the exception of ruminal VFA concentrations and lower intramuscular fat content.

  1. Improved Differential Evolution Algorithm for Parameter Estimation to Improve the Production of Biochemical Pathway

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    Chuii Khim Chong

    2012-06-01

    Full Text Available This paper introduces an improved Differential Evolution algorithm (IDE which aims at improving its performance in estimating the relevant parameters for metabolic pathway data to simulate glycolysis pathway for yeast. Metabolic pathway data are expected to be of significant help in the development of efficient tools in kinetic modeling and parameter estimation platforms. Many computation algorithms face obstacles due to the noisy data and difficulty of the system in estimating myriad of parameters, and require longer computational time to estimate the relevant parameters. The proposed algorithm (IDE in this paper is a hybrid of a Differential Evolution algorithm (DE and a Kalman Filter (KF. The outcome of IDE is proven to be superior than Genetic Algorithm (GA and DE. The results of IDE from experiments show estimated optimal kinetic parameters values, shorter computation time and increased accuracy for simulated results compared with other estimation algorithms

  2. Inversion of Biochemical Parameters by Selection of Proper Vegetation Index in Winter Wheat

    Institute of Scientific and Technical Information of China (English)

    HUANG Wen-jiang; WANG Ji-hua; LIU Liang-yun; ZHAO Chun-jiang; WANG Zhi-jie; WANG Jin-di

    2004-01-01

    Recent studies have demonstrated the application of vegetation indices from canopy reflected spectrum for inversion of chlorophyll concentration.Some indices are both response to variations of vegetation and environmental factors.Canopy chlorophyll concentration,an indicator of photosynthesis activity,is related to nitrogen concentration in green vegetation and serves as an indicator of the crop response to soil nitrogen fertilizer application.The combination of normalized difference vegetation index (NDVI) and photochemical reflectance index (PRI) can reduce the effect of leaf area index (LAI) and soil background.The canopy chlorophyll inversion index (CCII) was proved to be sensitive to chlorophyll concentration and very resistant to the other variations.This paper introduced the ratio of TCARI/OSAVI to make accurate predictions of winter wheat chlorophyll concentration under different cultivars.It indicated that canopy chlorophyll concentration could be evaluated by some combined vegetation indices.

  3. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

    Directory of Open Access Journals (Sweden)

    Ye. Barylo

    2016-06-01

    Full Text Available Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae of brown trout, rainbow trout and brook trout we used as study materials. Morphometric parameters we studied by the methods of N. O. Lange, E. N. Dmitrieva. The content of total lipids was determined in accordance with Folch. in the tissuesm, which were taken for biochemical studies. Separate classes of lipids were received by thin layer chromatography. Findings. We carried out a comparative analysis of morphometric measurements and biochemical parameters of one-day pre-larval brown trout, rainbow trout and brook trout based on the obtained data. We investigated morphometric and biochemical specific features of pre-larvae in post-embryonic period and showed the species differences of morphometric measurements. Significant differences were observed between the content of lipids in the body and yolk sac of free embryos. In particular, a higher content of phospholipids and triglycerides was observed in the body of brook trout compared to brown trout. We also recorded higher contents of mono- and diacylglycerols, free cholesterol, non-etherified fatty acids (NEFA, triacylglycerols and cholesterol esters in the yolk sac of brook trout. Compared to brown trout, rainbow trout had a significant increase in mono- and diacylglycerols, free cholesterol and NEFA in both body and yolk sac as well higher levels of total lipids, triacylglycerols and cholesterol esters were registered in yolk sac. Originality. For the first time we carried out and compared the specific features of pre-larval brown trout, rainbow trout and brook trout in the

  4. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

    Directory of Open Access Journals (Sweden)

    AGRIPINA SAPCALIU

    2013-12-01

    Full Text Available Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination. The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth condition of the respective colony. All the samples coming from sick colonieswere removed, only healthy adult honeybees coming from strong colonies were keptand used for hemolymph collection. By special methods, samples of hemolymph (300μl/sample collected from about 50 individuals, were analyzed both during the activeseason (spring/summer and the inactive season (autumn/winter. The study wascarried out on 50 samples of undiluted hemolymph taken from a total number of about2,500 honeybees. The following 21 biochemical parameters were analyzed: GLU(mg/dl, HDL-c (mg/dl, ALP (UI/l, T-cho (mg/dl, Tprot (mg/dl, Alb. (g/dl, BUN(mg/dl, LDH (UI/l, CPK (UI/l, Mg (mg/dl, FRA (μm/l, IP (mg/dl, GGT (UI/l,GOT (UI/l, GPT (UI/l, Ca (mg/dl, Cre (mg/dl, Amy (UI/l, T–BIL (mg/dl, TG(mg/dl, UA (mg/dl. The test was carried out after the collection and processing of thesamples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique,respectively.

  5. Long-term changes in microbial and biochemical parameters in the Central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Raghukumar, C.; Nath, B.N.; Sharma, R.; LokaBharathi, P.A.; Dalal, S.G.

    an artificial sediment disturbance was created by a hydraulic benthic disturber. Besides the above- mentioned parameters, extracellular enzymes, alkaline phosphatase, aminopeptidase and lipase were also monitored at these 9 locations. Postdisturbance (PoD1...

  6. Rutin restores adenosine deaminase activity in serum and the liver and improves biochemical parameters in streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    E.O. CHIELLE

    2016-01-01

    Full Text Available ABSTRACT denosine deaminase (ADA is a critical control point in the regulation of adenosine levels. This study aimed to investigate the effects of a polyphenolic flavonoid, rutin, on the activity of ADA in serum, the cerebral cortex, liver, kidney, and biochemical parameters in diabetic rats. The animals were divided into four groups (n=6 for the following treatments: control; diabetic (streptozotocin 55 mg/kg; diabetic with rutin (100 mg/kg/day; diabetic with glibenclamide (10 mg/kg/day. After 30 days, ADA activity and biochemical parameters were analyzed. The ADA activity in the serum was significantly elevated in the diabetic group compared to the control group (p<0.01. The treatment with rutin prevented the increase in ADA activity in the STZ-induced rats when compared to control group. Our data showed that rutin reduced glucose, LDL levels, and hepatic enzymes in comparison with the control group. These results demonstrate that the increase of ADA activity observed in diabetic rats may be an important indicator of the immunopathogenesis of hyperglycemic disorders and suggest that rutin is important for regulating the enzymatic activities associated with immune, hyperglycemic, and inflammatory response in diabetes mellitus.

  7. Therapeutic competence of dried garlic powder (Allium sativum on biochemical parameters in lead (Pb exposed broiler chickens

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    Md. Anwar Hossain

    2014-12-01

    Full Text Available The study was conducted to assess the therapeutic competence of garlic (Allium sativum in lead (Pb exposed chickens. The experimental birds (n=350 were grouped into T0 (as control, T1, T2, T3 and T4. The birds of group T1 was provided with lead acetate at 100 mg/kg body weight. Group T2 had lead acetate at 100 mg/kg b.wt. + 1% garlic supplement, whereas group T3 was fed with lead acetate at 100 mg/kg b.wt. + 2% garlic supplement, and group T4 had lead acetate at 100 mg/kg b.wt. + 4% garlic supplement. The mean values (mg/dL of uric acid, blood urea nitrogen, creatinine, cholesterol, triglycerides, low density lipoprotein, high density lipoprotein, and blood glucose in the birds of group T1 were significantly increased (p<0.01 on day 42 of post-treatment. Elevation of these parameters was suggestive for the pathological involvement of different organs like liver, kidney, muscles. Statistical analysis of variance indicated that lead acetate at 100 mg/kg b.wt. + 2% garlic supplement (T3 resulted significant (p<0.01 ameliorative effect on the biochemical parameters as compared to the group T2 and T4. In conclusion, potency of garlic in reversion of the values of the biochemical properties in Pb exposed chickens was close to the normal levels of the values.

  8. Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters

    DEFF Research Database (Denmark)

    Ferk, Franziska; Mišík, Miroslav; Nersesyan, Armen

    2016-01-01

    SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out...... if XN causes alterations of health-related parameters in humans. METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability......'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism...

  9. Early detection of crop injury from herbicide glyphosate by leaf biochemical parameter inversion

    Science.gov (United States)

    Zhao, Feng; Guo, Yiqing; Huang, Yanbo; Reddy, Krishna N.; Lee, Matthew A.; Fletcher, Reginald S.; Thomson, Steven J.

    2014-09-01

    Early detection of crop injury from herbicide glyphosate is of significant importance in crop management. In this paper, we attempt to detect glyphosate-induced crop injury by PROSPECT (leaf optical PROperty SPECTra model) inversion through leaf hyperspectral reflectance measurements for non-Glyphosate-Resistant (non-GR) soybean and non-GR cotton leaves. The PROSPECT model was inverted to retrieve chlorophyll content (Ca+b), equivalent water thickness (Cw), and leaf mass per area (Cm) from leaf hyperspectral reflectance spectra. The leaf stress conditions were then evaluated by examining the temporal variations of these biochemical constituents after glyphosate treatment. The approach was validated with greenhouse-measured datasets. Results indicated that the leaf injury caused by glyphosate treatments could be detected shortly after the spraying for both soybean and cotton by PROSPECT inversion, with Ca+b of the leaves treated with high dose solution decreasing more rapidly compared with leaves left untreated, whereas the Cw and Cm showed no obvious difference between treated and untreated leaves. For both non-GR soybean and non-GR cotton, the retrieved Ca+b values of the glyphosate treated plants from leaf hyperspectral data could be distinguished from that of the untreated plants within 48 h after the treatment, which could be employed as a useful indicator for glyphosate injury detection. These findings demonstrate the feasibility of applying the PROSPECT inversion technique for the early detection of leaf injury from glyphosate and its potential for agricultural plant status monitoring.

  10. Effect of maternal exercise on biochemical parameters in rats submitted to neonatal hypoxia-ischemia.

    Science.gov (United States)

    Marcelino, Thiago Beltram; de Lemos Rodrigues, Patrícia Idalina; Miguel, Patrícia Maidana; Netto, Carlos Alexandre; Pereira Silva, Lenir Orlandi; Matté, Cristiane

    2015-10-05

    Pregnancy is a critical period for brain metabolic programming, being affected by individual environment, such as nutrition, stress, and physical exercise. In this context, we previously reported a cerebral antioxidant upregulation and mitochondrial biogenesis in the offspring delivered from exercised mothers, which could provide neuroprotection against neonatal insults. Hypoxia-ischemia (HI) encephalopathy is one of the most studied models of neonatal brain injury; disrupting motor, cognitive, and learning abilities. Physiopathology includes oxidative stress, allied to mitochondria energy production failure, glutamatergic excitotoxicity, and cell death. In this study we evaluated the effect of maternal swimming during pregnancy on offspring׳s brain oxidative status evaluated fourteen days after HI stablishment. Swimming exercise was performed by female adult rats one week before and during pregnancy, in controlled environment. Their offspring was submitted to HI on postnatal day 7, and the brain samples for biochemical assays were obtained in the weaning. Contrary to our expectations, maternal exercise did not prevent the oxidative alterations observed in brain from HI-rats. In a general way, we found a positive modulation in the activities of antioxidant enzymes, measured two weeks after HI, in hippocampus, striatum, and cerebellum of pups delivered from exercised mothers. Reactive species levels were modulated differently in each structure evaluated. Considering the scenery presented, we concluded that HI elicited a neurometabolic adaptation in both brain hemispheres, particularly in hippocampus, parietal cortex, and cerebellum; while striatum appears to be most damaged. The protocol of aerobic maternal exercise was not enough to fully prevent HI-induced brain damages.

  11. Evaluation of Some Biochemical Parameters and Brain Oxidative Stress in Experimental Rats Exposed Chronically to Silver Nitrate and the Protective Role of Vitamin E and Selenium

    Science.gov (United States)

    Gueroui, Mouna; Kechrid, Zine

    2016-01-01

    Due to undesirable hazardous interactions with biological systems, this investigation was undertaken to evaluate the effect of chronic exposure to silver on certain biochemical and some oxidative stress parameters with histopathological examination of brain, as well as the possible protective role of selenium and/or vitamin E as nutritional supplements. Thirty six male rats were divided into six groups of six each: the first group used as a control group. Group II given both vitamin E (400 mg/kg) of diet and selenium (Se) (1 mg/L) in their drinking water. Group III given silver as silver nitrate (AgNO3) (20 mg/L). Group IV given vitamin E and AgNO3. Group V given both AgNO3 and selenium. Group VI given AgNO3, vitamin E and Se. The animals were in the same exposure conditions for 3 months. According to the results which have been obtained; there was an increase in serum lactate dehydrogenase (LDH), lipase activities and cholesterol level, a decrease in serum total protein, calcium and alkaline phosphatase (ALP) activity in Ag-intoxicated rats. Moreover, the findings showed that Ag+ ions affected antioxidant defense system by decreasing superoxide dismutase (SOD) activity and increasing vitamin E concentration with a high level of malondialdehyde (MDA) in brain tissue. The histological examination also exhibited some nervous tissue alterations including hemorrhage and cytoplasm vacuolization. However, the co-administration of selenium and/or vitamin E ameliorated the biochemical parameters and restored the histological alterations. In conclusion, this study indicated that silver could cause harmful effects in animal body and these effects can be more toxic in high concentrations or prolonged time exposure to this metal. However, selenium and vitamin E act as powerful antioxidants which may exercise adverse effect against the toxicity of this metal.

  12. [Serum leptin concentration and some lipid parameters in vegetarian children].

    Science.gov (United States)

    Laskowska-Klita, Teresa; Ambroszkiewicz, Jadwiga; Klemarczyk, Witold

    2004-04-01

    Leptin, a hormone from adipose tissue, regulates feeding behavior, satiation rate, energy expenditure and also plays an important role in maturation and reproduction. Recent studies support the concept that several factors such as a diet may influence on leptin levels. The aim of this study was to investigate serum concentration of leptin and lipids status in prepubertal children aged from 2 to 10 years with two different nutritional habits: vegetarian (n = 24) and omnivorous diet (n = 20). Serum leptin concentration was determined by immunoenzymeassay (ELISA). Serum lipids (cholesterol, HDL- and LDL-cholesterol, triglyceride) were measured by enzymatic and apolipoproteins by immunoturbidimetric methods. We noticed that in vegetarian diet there is a high rate of fiber (nearly twice as high as in omnivorous diet) and polyunsaturated acids (35% as much). In our study vegetarian children had lower total cholesterol and cholesterol in fractions HDL and LDL than meat eaters did. Also the apolipoproteins levels in vegetarian children were significantly below that of nonvegetarians. There is no differences in triglyceride concentration between the two groups of children. The mean serum leptin level in vegetarian children was significantly lower (3.1 +/- 1.2 ng/mL) as compared with the omnivores (5.6 +/- 2.1 ng/mL) (p < 0.0001).

  13. Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

    Science.gov (United States)

    Brito, Paula; Antunes, Fernando

    2014-10-01

    The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity towards H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underline the interplay between oxidative stress and redox signaling responses.

  14. Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

    Directory of Open Access Journals (Sweden)

    Paula Matos de Brito

    2014-10-01

    Full Text Available The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity towards H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underline the interplay between oxidative stress and redox signaling responses.

  15. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    Energy Technology Data Exchange (ETDEWEB)

    Raposo, F.; Borja, R.; Rincon, B. [Instituto de la Grasa (CSIC), Avda. Padre Garcia Tejero 4, 41012 Seville (Spain); Jimenez, A.M. [Departamento de Ciencias Ambientales, Universidad Pablo de Olavide, Ctra. De Utrera, km 1, 41013 Sevilla (Spain)

    2008-12-15

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (Y{sub M,ult}) decreased considerably from 227{+-}23 to 107{+-}11 ml CH{sub 4} at standard temperature and pressure (STP) conditions g{sup -1} VS{sub added} when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g{sup -1} VS{sub added} was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates. (author)

  16. Evaluation of serum biochemical marker concentrations and survival time in dogs with protein-losing enteropathy

    NARCIS (Netherlands)

    Equilino, Mirjam; Théodoloz, Vincent; Gorgas, Daniela; Doherr, Marcus G.; Heilmann, Romy M.; Suchodolski, Jan S.; Steiner, JöRg M.; Burgener, Iwan A.

    2015-01-01

    Results—Serum C-reactive protein concentration was high in 13 of 18 dogs with PLE and in 2 of 18 dogs with FRD. Serum concentration of canine pancreatic lipase immunoreactivity was high in 3 dogs with PLE but within the reference interval in all dogs with FRD. Serum a1-proteinase inhibitor concentra

  17. Aluminium-induced changes in hemato-biochemical parameters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid.

    Science.gov (United States)

    Yousef, Mokhtar I

    2004-06-01

    For a long time, aluminium (Al) has been considered an indifferent element from a toxicological point of view. In recent years, however, Al has been implicated in the pathogenesis of several clinical disorders, such as dialysis dementia, the fulminant neurological disorder that can develop in patients on renal dialysis. Therefore, the present experiment was carried out to determine the effectiveness of l-ascorbic acid (AA) in alleviating the toxicity of aluminium chloride (AlCl3) on certain hemato-biochemical parameters, lipid peroxidation and enzyme activities of male New Zealand white rabbits. Six rabbits per group were assigned to 1 of 4 treatment groups: 0mg AA and 0mg AlCl3/kg body weight (BW) (control); 40 mg AA/kg BW; 34 mg AlCl3/kg BW (1/25 LD50); 34 mg AlCl3 plus 40 mg AA/kg BW. Rabbits were orally administered their respective doses every other day for 16 weeks. Evaluations were made for lipid peroxidation, enzyme activities and hemato-biochemical parameters. Results obtained showed that AlCl3 significantly (PAluminium treatment caused a significant decrease in plasma total lipids (TL), blood haemoglobin (Hb), total erythrocytic count (TEC) and packed cell volume (PCV), and increased total leukocyte count (TLC) and the concentrations of glucose, urea, creatinine, bilirubin and cholesterol. Ascorbic acid alone significantly decreased the levels of free radicals, TL, cholesterol, glucose and creatinine, and increased the activity of GST, SH groups, Hb, TEC and PCV. While, the rest of the tested parameters were not affected. Also, the present study showed that ascorbic acid can be effective in the protection of aluminium-induced toxicity.

  18. Selected haematological and biochemical parameters of blood in rats after subchronic administration of vanadium and/or magnesium in drinking water.

    Science.gov (United States)

    Scibior, Agnieszka; Zaporowska, Halina; Ostrowski, Jarosław

    2006-08-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg.

  19. Stochastic analyses of field-scale pesticide leaching risk as influenced by spatial variability in physical and biochemical parameters

    Science.gov (United States)

    Loll, Per; Moldrup, Per

    2000-04-01

    Field-scale pesticide leaching risk assessments were performed by incorporating a numerical, one-dimensional, water and pesticide transport and fate model into the two-step stochastic modeling approach by Loll and Moldrup [1998]. The numerical model included first-order pesticide degradation, linear equilibrium adsorption, and plant uptake of water and pesticide. Simazine was used as a model pesticide, and leaching risk was expressed as the cumulative mass fraction of applied pesticide leached below 100 cm after 1 year. Spatial variability in soil physical and biochemical data, as well as measured meteorological data from an average and a relatively wet year, was considered for two Danish field sites: (1) a coarse sandy soil, with relatively small variability in hydraulic properties, and (2) a sandy loam, with large variability in hydraulic properties. The two-step stochastic modeling approach was used to investigate the relative impact of spatial variability in saturated hydraulic conductivity Ks, soil-water retention through the Campbell [974] soil-water retention parameter b, and pesticide sorption through the organic carbon content (OC). For the coarse sandy soil, field-scale spatial variability in OC was the single most important parameter influencing leaching risk, whereas for the sandy loam, Ks was found more important than OC. The relative impact of field-scale spatial variability in these parameters was found independent of the meteorological conditions, whereas the absolute level of leaching risk was highly dependent on the meteorological conditions. Assuming a linear dependency between pesticide half-life and OC, a unified approach to modeling simultaneous field-scale variability in biodegradation and adsorption was proposed. Leaching risk assessments based on this approach showed that the parts of the field with both low biological activity and low adsorption capacity contributed with a dramatic increase in leaching risk, and suggested that field

  20. Haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria

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    A.O. Omonona

    2011-12-01

    Full Text Available The haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria was carried out with the view of establishing baseline blood health indices of this species and generating data which could be useful in the comparative physiology of turtles. A total of sixty free ranging turtles comprising juveniles and adults of both sexes were used for the study. The mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in male juvenile were significantly higher (P < 0.05 than those of the females. Nevertheless, in adult turtles, the mean values for the RBC, PCV, Hb, MCV, MCH and WBC counts observed in females were significantly higher (P < 0.05 than those of the males. Similarly, in juvenile turtles, the absolute heterophil, lymphocyte and monocyte counts in females were relatively higher (P < 0.05 than that of male while in adult turtles there were no significant differences (P < 0.05 in these parameters between the males and females. There were no significant difference (P < 0.05 in the values for the total protein, albumin, globulin, creatinine, serum glutamic-oxalacetic transaminase (SGOT, serum glutamic-pyruvic transaminase (SGPT and blood urea nitrogen in the males and females of both the juvenile and adult turtles. The outcome of this study presents baseline data on the haematology and serum biochemical parameters in free-ranging African side neck turtle (Pelusios sinuatus in Ibadan, Nigeria, which could also serve as a template for the comparative physiology of fresh and sea turtles.

  1. Single versus repeated tramadol injection in laparotomized albino rats: comparison of effects on hematology, serum biochemical parameters, and body weight gain

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    Rita Ijeoma Udegbunam

    2015-09-01

    Full Text Available This study was aimed to assess the effects of single and repeated tramadol administration on some hematological and serum biochemical parameters of laparotomized rats. Laparotomized rats (n=18 were randomly divided into three equal groups. Normal saline was administered to the rats of group 1 (control. Tramadol (dosed at 10 mg/kg bwt was administered singly to the rats of group 2. The same dose of tramadol was administered repeatedly every 12 h up to 72 h to the rats of group 3. On day 3 and 10 post-laparotomy (PSL, blood urea nitrogen, serum creatinine, total serum protein, hematocrit, hemoglobin concentration and red blood cell counts in the rats of group 2 were found to be significantly (P<0.05 higher than those obtained in group 1 and group 3. Mean weights of rats in group 1 and group 3 were significantly lower than those of the rats of group 2 PSL. This study showed that repeated tramadol administration lead to slower weight gain as well as marked decrease in biochemical and erythrocytic variables in rats. Therefore, single administration of tramadol PSL may suffice for analgesia. [J Adv Vet Anim Res 2015; 2(3.000: 316-320

  2. Model reduction and parameter estimation of non-linear dynamical biochemical reaction networks.

    Science.gov (United States)

    Sun, Xiaodian; Medvedovic, Mario

    2016-02-01

    Parameter estimation for high dimension complex dynamic system is a hot topic. However, the current statistical model and inference approach is known as a large p small n problem. How to reduce the dimension of the dynamic model and improve the accuracy of estimation is more important. To address this question, the authors take some known parameters and structure of system as priori knowledge and incorporate it into dynamic model. At the same time, they decompose the whole dynamic model into subset network modules, based on different modules, and then they apply different estimation approaches. This technique is called Rao-Blackwellised particle filters decomposition methods. To evaluate the performance of this method, the authors apply it to synthetic data generated from repressilator model and experimental data of the JAK-STAT pathway, but this method can be easily extended to large-scale cases.

  3. Cytomorphologic spectrum of lymphocytic thyroiditis and correlation between cytological grading and biochemical parameters

    OpenAIRE

    Anila, K. R.; Nileena Nayak; K Jayasree

    2016-01-01

    Introduction: Chronic lymphocytic thyroiditis [Hashimoto thyroiditis (HT)] is a common thyroid lesion diagnosed on fine-needle aspiration cytology (FNAC). Apart from FNAC, various other parameters, such as clinical features, ultrasonographic findings, antithyroid antibody levels, hormone profiles, and radionuclide thyroid scan, are also taken into consideration in making a diagnosis of HT. Aims: To grade lymphocytic thyroiditis based on the cytomorphology and to correlate the cytological ...

  4. Pharmacokinetics of cefpodoxime proxetil with special reference to biochemical parameters, tissue residue, and spermatozoa motility in rats

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    Momin A Mujeeb

    2011-01-01

    Full Text Available Background : Cefpodoxime is a semisynthetic third generation cephalosporin analogue with a relatively broader spectrum of antimicrobial activity against gram negative and gram positive organisms. This is attributed to their somewhat increased resistance to degradation by the betalactamase. Cefpodoxime shows good activity against Klebsiella pneumonia, many members of enterobactericeae and almost all strains of Escherichia coli. It is extensively used in human beings against infections caused by susceptible organisms for a prolonged period and even without its judicious indication. Though various researchers have worked on the pharmacokinetic aspects of the drug, its effects on biochemical parameters and spermatozoa activity are scarcely available in literature. Aim : To determine the oral kinetic ( blood and tissue after single therapeutic dose of cefpodoxime proxetil (20mg/kg oral bid 7 days in rats of either sex on tissue half life and certain biochemical parameters such as glucose, hemoglobin, protein, ALT, AST and other parameters like tissue residue, sperm count and spermatozoa motility in male rats. Materials and Methods : For kinetic studies,24 Wister rats of either sex, 3 months of age, (180-210 gm were used.(Group I-IV; n=6 Blood samples collected from each animal of Group IV through heart puncture at 0 hour to serve as predrug control. All the group (I-IV received cefpodoxime proxetil 20 mg/kg once orally as a single dose. At the end of 1,4,12 and 24 hour post oral administration, GroupI,II,III and IVwere utilized for kinetic studies. Blood samples were collected from each animal and vital organs viz brain, lung, liver, spleen, kidney and heart were dissected out for drug analysis and determination of weight. For biochemical parameters, tissue residue and spermatozoa motility, twelve male rats were randomly divided into Groups A and B (n=6 Group B received cefpodoxime (20mg/kg orally bid 7 days while Group A served as control

  5. Utility and limitations of biochemical parameters in the evaluation of hyponatremia in the elderly.

    Science.gov (United States)

    Musch, W; Decaux, G

    2001-01-01

    We evaluated in 110 consecutive elderly hyponatremic patients the value of traditional clinical and biochemical data and the place of a test infusion of 2 liters isotonic saline over 24 hours, in establishing the etiology of the hyponatremia. The causes of hyponatremia were as follows: 31% SIADH patients, 23% patients with hyponatremia due to diuretics, 18% potomania patients, 15% salt depleted patients, 5% salt depleted SIADH patients, 5% patients with a salt loosing syndrome and 3% patients with hyponatremia of unknown origin. Several salt depleted (SD) and SIADH patients could be confounded. Usually, adults with SIADH show plasma uric acid values 4 mg/dL. Eighty-two percent of SD patients appeared to have plasma urea levels >30 mg/dL, but this was also the case in 21% of SIADH patients. Twenty-nine of the SD patients presented a urinary sodium >30 mEq/L, but all had fractional sodium excretion (FENa) lower than 0.5%. However, in SIADH, 42% of the patients presented also FENa potomania patients, salt loosing syndrome patients and some SD SIADH patients). A plasma sodium (PNa) increase of at least 5 mEq/L 24 hours after saline infusion has been suggested as highly suggestive of SD. Nevertheless, 29% of our SD patients did not increase their PNa level by 5 mEq/L or more, while 30% of our SIADH patients did. PNa improved after 2 liters isotonic saline over 24 hours in 90 patients (85%) as opposed to 12 others (9 SIADH and 3 diuretic patients), decreasing their plasma sodium. The isotonic saline infusion test, only allows a reliable classification of hyponatremia, as far as both PNa and sodium excretion were taken into account. In the SIADH group, 6 patients (5%) presented initially manifest solute depletion and retained the 2 liters isotonic saline before developping inappropriate natriuresis. Six patients showed a transient salt loosing syndrome with high fractional potassium excretion (FEK) and high calciuria, which differentiates them from thiazide patients

  6. Effect of Aqueous Stem Bark Extract of Khaya senegalensis on Some Biochemical, Haematological, and Histopathological Parameters of Rats.

    Science.gov (United States)

    Onu, A; Saidu, Y; Ladan, M J; Bilbis, L S; Aliero, A A; Sahabi, S M

    2013-01-01

    The subchronic effect of aqueous stem bark extract of Khaya senegalensis on some biochemical, haematological, and histopathological parameters of rats was investigated. The rats were divided into six groups of five rats per group. Groups I to VI were administered graded doses of 0, 400, 800, 1200, 1600, and 2000 mg/kg bw, respectively. The result of study revealed that administration of the Khaya senegalensis for twenty-eight days at the experimental dose resulted in significant (P < 0.05) increase in urea, electrolytes (Na(+), K(+)), and creatinine levels. The extract also significantly (P < 0.05) increased serum activity of ALT, AST, and ALP. The levels of protein, albumin, and bilirubin were significantly changed when compared to their control values, but they were not dose dependent. The hematological indices assayed in this study were not significantly affected at the experimental dose when compared to the control values. Histological studies of the liver showed cellular degeneration and necrosis and bile duct hyperplasia and fibrosis with lymphocytic infiltration of the hepatocyte, providing supportive evidence for discussing the biochemical findings, indicative of functional derangement. The histological architecture of the kidney and that of the heart were however preserved. The result of this study indicates that the aqueous stem bark extract of K. senegalensis may affect the cellular integrity of vital organs of the body.

  7. Metal effects on histological and biochemical parameters of common rudd (Scardinius erythrophthalmus L.

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    Georgieva Elenka

    2014-09-01

    Full Text Available The present study aimed to evaluate the current contamination status of Topolnitsa Reservoir, which is located in a region with intensive copper mining. The reservoir has been continually contaminated with metals over the last few decades. As, Cd, Cu, Ni, Pb, and Zn concentrations were measured in surface water samples and in the liver of common rudd, Scardinius erythrophthalmus (L., in three different seasons: spring, summer, and autumn. The morphological structure of the fish liver was examined and the hepatic LDH, ALAT, and ASAT activities were measured. In general, metal concentrations in the water varied, but As and Cu were present in all three seasons at levels higher than the maximum permissible levels set by law. The metal concentrations in the fish liver were significantly higher than in the water. Histological alterations were classified as degenerative changes (granular, vacuolar, hydropic, and fatty degeneration, necrotic changes (necrobiosis - karyopyknosis, karryorehsis, and karyolyzis, and necrosis; and changes in blood vessels (hyperemia in sinusoids and major blood vessels. Higher LDH, ALAT, and ASAT activities in fish livers were measured in comparison to reference fish. In addition, the ALAT activity in the livers of S. erythrophthalmus from Topolnitsa Reservoir was significantly higher in the summer. Overall, it can be concluded that the metal-contaminated waters of Topolnitsa Reservoir lead to negative changes in the common rudd tissues at the cellular level including impaired structure and enhanced enzymatic activity in the fish liver.

  8. Use of simple hematological, biochemical and clinical parameters to monitor response of multiple myeloma patients on high dose thalidomide therapy

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    Uppal G

    2005-01-01

    Full Text Available BACKGROUND: Evidence of increased bone marrow vascularity in multiple myeloma (MM has led to the use of anti-angiogenic drugs especially thalidomide in relapsed or refractory patients. Currently, parameters such as serum/ urine electrophoresis for M (monoclonal proteins, bone marrow biopsy with touch preparation and b2 microglobulin are routinely used to assess response to therapy. These investigations are expensive, invasive and require high technical setup. AIM: To correlate simple and routine hematological and biochemical parameters with the key marker of disease i.e. M proteins. SETTINGS AND DESIGN: This is an open label, uncontrolled, single-arm study. MATERIALS AND METHODS: Twenty nine refractory or relapsed multiple myeloma patients of both sexes (M=20, F=9 with age ranging between 35-72 years were initiated on 200 mg/day of thalidomide with fortnightly increments of 200 mg to a maximum tolerated dose not exceeding 800 mg/day. All hematological and biochemical parameters were monitored at monthly intervals for one year. STATISTICAL ANALYSIS: Correlation analysis was performed between hemoglobin (Hb, total leukocyte count (TLC, absolute neutrophil count (ANC, platelet count (PC, total proteins (TP, serum albumin and serum globulin on one hand and M protein levels on the other using Pearsons Correlation test by SPSS version 7.5. RESULT: Hb, TLC, ANC, PC and serum albumin levels showed a significant negative correlation with M proteins. A highly significant positive correlation existed between M proteins on one hand and TP and globulin levels on the other. Dryness of skin indicated positive response to therapy. These correlations were found to be significant at the end of one month of therapy in all the above-mentioned parameters except in TLC where it was significant after 2 months of thalidomide therapy. CONCLUSION: Results suggest that sustained efficacy of thalidomide therapy may be amenable to monitoring by these simple, inexpensive and

  9. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

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    Danila Di Majo

    2014-12-01

    Full Text Available In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT] and skeletal and myocardial markers of muscle damage [creatin kinase (CK and creatin kinase MB (CK-MB]. Endurance training, as it implies high oxygen consumption, should increase reactive oxygen species, but it has been shown that exercise leads to increased activation of antioxidant defenses. In fact, serum levels of γ-GT enzyme and total CK were not increased. On the other hand, a statistical significant reduction of CKMB has been observed. There were not variations in hematological parameters. As far as the anthropometric value is concerned, after two months of training there was a change in weight (P<0.0001. Finally, any oxidative and biological stress was highlighted in the middle distance runners but, since this is a preliminary study, it would be of interest to replicate the study on a larger sample.

  10. Evaluation of nanoselenium (Nano-Se) effect on hematological and serum biochemical parameters of rat in experimentally lead poisoning.

    Science.gov (United States)

    Jafari Dehkordi, A; Mohebbi, A N; Aslani, M R; Ghoreyshi, S M

    2016-06-01

    The present study was designed to evaluate the effect of nanoselenium (Nano-Se) on hematological and biochemical parameters of rats experimentally intoxicated with lead (Pb). Thirty male rats were randomly divided into six groups as follows: the control, selenite, Nano-Se, Pb group, Pb + selenite, and Pb + Nano-Se groups. After 35 days, blood was collected from rats and hematology and serum biochemical parameters of oxidative stress were measured. The thiobarbituric acid reactive substances (TBARS) level of Pb group was significantly higher than other groups. Also, TBARS level was significantly lower in the Pb + Nano-Se group than Pb + selenite group. The serum superoxide dismutase activities were significantly lower in Pb group than the control, Pb + selenite, and Pb + Nano-Se groups. The catalase activities in the Pb group showed no significant change when compared to other groups. In the Pb group, packed cell volume was lower than the control group. A significant difference was observed between the control group and the Pb, Pb + selenite, and Pb + Nano-Se groups. In the Pb group, the numbers of white blood cell (WBC) decreased in comparison with the control group. Also, there was significant increase in WBC counts in the Pb + Nano-Se and Pb + selenite groups in comparison with Pb group. The number of lymphocytes in the Pb group decreased in comparison with the control group. By comparing the means of the Pb + Nano-Se and Pb + selenite groups together, it was determined that there were significant differences in the lymphocytes and neutrophil counts. In conclusion, usage of selenium compounds particularly Nano-Se particles inhibits the adverse effects of Pb on antioxidant activity and immune system function in the Pb poisoning.

  11. Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system.

    Science.gov (United States)

    Bubel, F; Dobrzański, Z; Gaweł, A; Pogoda-Sewerniak, K; Grela, E R

    2015-01-01

    An influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C - standard feeding), and experimental, supplemented with prepara- tions: E-1 herbal-humic, E-2 humic-herbal and E-3--humic-lucerne. Hens were placed in the pens on the 16th week of life, addition of preparations with standard food mixture started at the 22nd wk and lasted until 66th wk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65th wk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminot- ransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne prepara- tion affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

  12. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries

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    A Kaur

    2012-01-01

    Full Text Available Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.

  13. Prematurity and Related Biochemical Outcomes: Study of Bone Mineralization and Renal Function Parameters in Preterm Infants

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    Sarika Singh Chauhan

    2011-01-01

    phosphorus levels were found to be significantly decreased, and serum ALP, creatinine, and electrolytes were found to be significantly increased (<0.001 at 28–30 weeks as compared to controls, but serum calcium and phosphorous levels were found to be insignificantly decreased, whereas serum ALP activities were found to be insignificantly increased at 28–30 weeks as compared to 30–32 weeks of gestational age in preterm babies. It can be concluded that high serum ALP activity and low serum calcium and phosphorus levels are associated with preterm babies. A significant difference in the mean values of these renal function parameters was also obtained, except for serum sodium and potassium.

  14. Association of Apo-E gene polymorphism with biochemical and lipid metabolism parameters in patients with diabetic nephropathy of Hui and Han populations in Gansu Province

    Institute of Scientific and Technical Information of China (English)

    郭茜

    2006-01-01

    Objective To study the association of apolipoprotein (Apo) E gene polymorphism with difference in biochemical metabolism of diabetic nephropathy of Hui and Han populations. Methods ApoE genotype was determined by PCR-RFLP in diabetic patients with or without diabetic nephropathy (DN) and normal peoples in Hui and Han peoples, the related biochemical parameters were simultaneously detected. Results (1) Huis had 3 genotypes, i. e. E2/E3, E3/E3 and E3/E4, and their fre-

  15. Cytomorphologic spectrum of lymphocytic thyroiditis and correlation between cytological grading and biochemical parameters

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    K R Anila

    2016-01-01

    Full Text Available Introduction: Chronic lymphocytic thyroiditis [Hashimoto thyroiditis (HT] is a common thyroid lesion diagnosed on fine-needle aspiration cytology (FNAC. Apart from FNAC, various other parameters, such as clinical features, ultrasonographic findings, antithyroid antibody levels, hormone profiles, and radionuclide thyroid scan, are also taken into consideration in making a diagnosis of HT. Aims: To grade lymphocytic thyroiditis based on the cytomorphology and to correlate the cytological grades with the levels of antithyroid peroxidase antibody (ATPO, antithyroglobulin antibody (ATG, and thyroid stimulating hormone (TSH. Materials and Methods: During a period of one and half years, 1,667 cases underwent FNAC of thyroid at our tertiary care center. Of these, 128 cases had cytological evidence of lymphocytic thyroiditis. Out of these, in 60 cases the levels of ATPO, ATG, and TSH were known. The cytological grades of lymphocytic thyroiditis in these cases were correlated with these parameters. Results: Out of the 60 cases, 55 were females. Age ranged from 5 years to 70 years, with majority of patients in third decade. Diffuse enlargement of thyroid was the commonest presentation. However, 14 cases presented with nodular disease. Majority of the patients had grade 1 thyroiditis (27 cases, followed by grade 2 thyroiditis (22 cases. Cytomorphology was diagnostic of thyroiditis in all 60 cases. ATPO was elevated in 57 cases and ATG was elevated in 40 cases. Elevated level of TSH was seen in only 18 cases. In 39 cases, TSH value was normal. There was no correlation between the cytological grades of thyroiditis and the levels of antithyroid antibodies and TSH. Conclusion: Lymphocytic infiltration of thyroid follicles is pathognomonic of lymphocytic thyroiditis. Positivity for antithyroid antibodies is strongly associated with HT but no correlation was observed between the grades of thyroiditis and the levels of ATPO, ATG, and TSH.

  16. A New Data Analysis System to Quantify Associations between Biochemical Parameters of Chronic Kidney Disease-Mineral Bone Disease.

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    Mariano Rodriguez

    Full Text Available In hemodialysis patients, deviations from KDIGO recommended values of individual parameters, phosphate, calcium or parathyroid hormone (PTH, are associated with increased mortality. However, it is widely accepted that these parameters are not regulated independently of each other and that therapy aimed to correct one parameter often modifies the others. The aim of the present study is to quantify the degree of association between parameters of chronic kidney disease and mineral bone disease (CKD-MBD.Data was extracted from a cohort of 1758 adult HD patients between January 2000 and June 2013 obtaining a total of 46.141 records (10 year follow-up. We used an advanced data analysis system called Random Forest (RF which is based on self-learning procedure with similar axioms to those utilized for the development of artificial intelligence. This new approach is particularly useful when the variables analyzed are closely dependent to each other.The analysis revealed a strong association between PTH and phosphate that was superior to that of PTH and Calcium. The classical linear regression analysis between PTH and phosphate shows a correlation coefficient is 0.27, p<0.001, the possibility to predict PTH changes from phosphate modification is marginal. Alternatively, RF assumes that changes in phosphate will cause modifications in other associated variables (calcium and others that may also affect PTH values. Using RF the correlation coefficient between changes in serum PTH and phosphate is 0.77, p<0.001; thus, the power of prediction is markedly increased. The effect of therapy on biochemical variables was also analyzed using this RF.Our results suggest that the analysis of the complex interactions between mineral metabolism parameters in CKD-MBD may demand a more advanced data analysis system such as RF.

  17. The effects of cadmium on the biochemical and physiological parameters of Eruca sativa.

    Science.gov (United States)

    Kompe, Yasemin Ozdener; Sagiroglu, Ahmet

    2016-12-01

    In this study, Eruca sativa (Rocket) seedlings were treated with different cadmium (Cd) concentrations (0, 150, 300 and 450 μg ∙ g(-1)). The effects of Cd on lipid peroxidation, enzymatic (APx, CAT, GPX, SOD) and non-enzymatic antioxidants (total ascorbate, dehydroascorbate, ascorbate, non-protein thiol), fresh and dry masses, water content were determined. Also, Cd content of the leaves and the roots were analysed. The highest cadmium accumulation of leaves was at 450 μg ∙ g(-1) Cd treatment and the accumulation was 2.62 times greater than those in the roots. The translocation factor was 3.89 at 300 μg ∙ g(-1) Cd treatment. Cd treatments caused decreases of fresh, dry mass and water content of leaves and roots. Malondialdehyde content, which is an index of lipid peroxidation, was increased in proportion with the increase in Cd. While there was not change in the activity of GPX according to control, a decrease in activities of SOD, CAT and APX were observed with the increase of cadmium concentration. Although a significant increase in the amounts of non-protein thiol groups and proline were observed in 450 μg ∙ g(-1) Cd treated plants, Cd did not lead to a significant change in AsA, DHA and total AsA contents. According to the results of the research, E. sativa may be a Cd hyperaccumulator plant and we suggest that the plant may be a candidate plant for remediation of Cd-contaminated soil.

  18. INTERACTIVE EFFECTS OF SALINITY STRESS AND NICOTINAMIDE ON PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS ON FABA BEAN PLANT

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    Magdi T. Abdelhamid

    2013-09-01

    Full Text Available A possible survival strategy of plants under saline conditions is to use some compounds that could alleviate salt stress effect. One of these compounds is nicotinamide. The effect of exogenously application of nicotinamide with different concentrations (0, 200 and 400 mg/l on Vicia faba L. plant against different NaCl treatments (0, 50 and 100 mM NaCl was investigated at the wire house of the National Research Centre, Cairo, Egypt. Salinity stress reduced significantly plant height, dry weight of shoot, photosynthetic pigments, polysaccharides, total carbohydrates, total-N contents of shoot, seed yield, total carbohydrates & total crude protein of the yielded seeds compared with those of the control plants. In contrast, salinity induced marked increases in sucrose, total soluble sugars, total free amino acids, proline, lipid peroxidation product (MDA and some oxidative enzymes (polyphenol oxidase and peroxidase enzymes. Also, salinity stress increased Na+ contents with the decreases of other macro and micro elements contents (P, K+, Mg+, Ca2+, Fe2+, Mn2+, Zn2+ and Cu2+ of shoots and the yielded seeds of faba bean. Foliar spraying of nicotinamide alleviated the adverse effects of salinity stress through increased plant height, dry weight of shoot, photosynthetic pigments, polysaccharides, total carbohydrates, total-N contents of shoot and seed yield as well as, sucrose, total soluble sugars, total free amino acids and proline, compared with those of the corresponding salinity levels, while decreased lipid peroxidation product as MDA and the oxidative enzymes (polyphenol oxidase and peroxidase enzymes. Nicotinamide inhibited the uptake of Na+ and accelerated the accumulation of P, K+ , Mg+, Ca2+, Fe2+, Mn2+, Zn2+ and Cu2+ contents in the shoots of salt stressed plants and enhanced total carbohydrate and total crude protein percentage and solutes concentrations in seeds of salinity treated plants. 

  19. Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

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    Reka Tienken

    2015-09-01

    Full Text Available The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010 serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL, whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of energy to cows which were similar in body condition score had only minor effects on periparturient immune system function and the metabolism of those cows.

  20. Methods of mesophyll conductance estimation: its impact on key biochemical parameters and photosynthetic limitations in phosphorus-stressed soybean across CO2.

    Science.gov (United States)

    Singh, Shardendu K; Reddy, Vangimalla R

    2016-06-01

    Despite the development of various methods, the rapid estimation of mesophyll conductance (gm ) for a large number of samples is still a daunting challenge. Although the accurate estimation of gm is critical to partition photosynthetic limitations by stomatal (Ls ) and mesophyll (Lm ) conductance and by photo-biochemical (Lb ) processes, the impact of various gm estimation methods on this is ambiguous. As phosphorus (P) starvation and elevated CO2 (eCO2 ) strongly affect photosynthetic processes, their combined effect on the proportional changes in these limitations are not well understood. To investigate this, while also evaluating distinct recent methods of gm estimation sharing few common theories and assumptions, soybean was grown under a range of P nutrition at ambient and eCO2 . Methods significantly affected gm and carboxylation efficiency (VCmax ) but not other photosynthetic parameters. In all the methods, all photosynthetic parameters responded similarly to treatments. However, the percentage difference between VCmax assuming finite and infinite gm was highly inconsistent among methods. The primary mechanism responsible for P limitation to soybean photosynthesis was not CO2 diffusion limitations but Lb comprised of reduced chlorophyll, photochemistry and biochemical processes. The eCO2 decreased Lb but increased Lm without affecting Ls across leaf P concentration. Although each method explored advances of our understanding about gm variability, they all require assumptions of varying degrees, which lead to the discrepancy in the gm values. Among the methods, the oxygen sensitivity-based gm estimation appeared to be suitable for the quick assessment of a large number of samples or genotypes. Digital tools are provided for the easy estimation of gm for some methods.

  1. Basic biochemical, hematological and hormonal parameters for monitoring the health and nutritional status in athletes

    Directory of Open Access Journals (Sweden)

    Aritz Urdampilleta

    2014-09-01

    Full Text Available Sporting competitions are becoming more demanding in terms of intensity of effort, and this means controlling all aspects that affect athletic performance. Food, hydration and supplementation, before, during and after training or competition directly affect health, body composition, performance and recovery of the athlete. The assessment of nutritional status is required for proper advising of the athlete, through blood tests to control the process of adaptation to training. The aim of this paper is to provide practical tools for dietitiansnutritionists to control the health and nutritional status of athletes, as well as monitoring their adaptation to workloads and competition periods. Performing analytical tests to control of protein metabolism, lipid profile, ions, blood tests and iron metabolism, in addition to review some hormonal parameters, may be of interest in order to observe the potential existence of overtraining states. The correct understanding and interpretation of laboratory tests (under sports doctor’s supervision will be most important and useful for dietitiansnutritionists, performing dietary and nutritional advice to athletes, because it will determine the status of the athlete and propose different individual feeding strategies depending on the training phase and response.

  2. Influence of vegetarian and mixed nutrition on selected haematological and biochemical parameters in children.

    Science.gov (United States)

    Krajcovicová-Kudlácková, M; Simoncic, R; Béderová, A; Grancicová, E; Magálová, T

    1997-10-01

    To evaluate the health and nutritional status of children with two different nutritional habits, the authors examined 26 vegetarians (lacto- and lacto-ovo; an average period of vegetarianism 2.8 years) and 32 individuals on mixed diet (omnivores) in the age range 11-14 years. Vegetarian children had significantly lower erythrocyte number as well as reduced levels of haemoglobin and iron compared to omnivores. The average level of iron did not reach the lower limit of the physiological range and hyposiderinemia was found in 58% of vegetarians vs 9% of omnivores. Reduced iron levels were observed in spite of increased intake of vegetable iron sources and vitamin C (which facilitates the conversion to ferro-form). This reduction can be attributed to the absence of animal iron sources with high utilizability and to lower iron utilization in the presence of phytic acid (higher intake of grains compared to omnivores). The incidence of hypoalbuminemia and hypoproteinemia in vegetarian children was 38 and 12%, respectively, compared to 0% in omnivores. The protein mixture from milk, eggs and vegetable sources is complete, but vegetarian children had significantly reduced intake of milk and dairy products. Favourable lipid and antioxidant parameters in vegetarian children reflect the optimal nutrition composition with respect to the prevention of free radical diseases. Such a nutrition results in significantly lower levels of cholesterol and LDL-cholesterol compared to omnivores and significantly higher and over threshold values of essential antioxidants--vitamin C, vitamin E/cholesterol (more effective protection against LDL oxidation), beta-carotene, vitamin A.

  3. Alterations of biochemical parameters in malformed Indian rice frogs, Rana limnocharis from Southern Taiwan.

    Science.gov (United States)

    Chiu, Yuh-Wen; Wang, Shu-Yin; Wu, Jui-Pin; Huang, Da-Ji

    2011-11-01

    The purpose of this study is to investigate the factors that cause malformed frogs in upstream Kaoping river (KP site) and Tungkang river (T site) of Southern Taiwan. In this experiment, the activities of monooxygenase (MO), glutathione-S-transferase (GST), acetylcholinesterase (AchE) as well as the concentration of vitellogenin (Vg) in the liver were measured. Results show that activities of MO, GST and AchE, and Vg levels in normal frogs (male/female) were 0.09 +/- 0.02/0.09+/-0.01 deltaA min(-1) mg(-1) protein, 0.12 +/- 0.04/0.13 +/- 0.04 deltaA min(-1) mg(-1) protein, 6.13 +/- 2.69/6.01 +/- 2.09 U mg(-1) protein and 0.87 +/- 0.42/2.18 +/- 0.50 microg mg(-1) protein, respectively. Activities of MO, GST and AchE, and Vg levels in malformed frogs (male/female) were 0.15 +/- 0.04/0.21 +/- 0.07 deltaA min(-1) mg(-1) protein, 0.27 +/- 0.08/0.30 +/- 0.12 deltaA min(-1) mg(-1) protein, 4.59 +/- 2.71/5.19 +/- 3.74 U mg(-1) protein and 1.46 +/- 0.61/3.15 +/- 0.88 microg mg(-1) protein, respectively in KP site, and were 0.16 +/- 0.69/0.1 +/- 80.07 deltaA min(-1) mg(-1) protein, 0.21 +/- 0.07/0.24 +/- 0.08 deltaA min(-1) mg(-1) protein, 5.13 +/- 4.58/3.94 +/- 1.33 U mg(-1) protein and 2.23 +/- 1.47/4.11 +/- 1.63 microg mg(-1) protein, respectively in T site. These results indicate that male and female malformed frogs in both rivers upstream are found with higher activities. No significant difference in AchE activity was found between normal and malformed frogs in this investigation. It is therefore reasonable to speculate that the organic chemicals released from agricultural activities are presumable the main factors that lead to the malformation of frogs.

  4. Effects of static magnetic field exposure on hematological and biochemical parameters in rats

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    Salem Amara

    2006-11-01

    Full Text Available The present work was undertaken in order to investigate the effects of static magnetic field (SMF on growth rates, hematopoiesis, plasmatic proteins levels, glucose concentration, lactate dehydrogenase (LDH and transaminases activities in male rats. Sub-acute exposure of rats during 5 consecutive days to SMF (1h/day at 128mT induced an increase of plasma LDH activity (+38%, pEste estudo foi realizado com o obejtivo de investigar os efeitos do campo magnético estático (CMS nas taxas de crescimento, hematopoiese, concentrações de proteínas plasmáticas, glicemia, da desidrogenase lática (DHL e transaminases (alanina aminotransferase-ALT e aspartato aminotransferase-AST em ratos machos. Após exposição de modo sub-agudo durante 5 dias consecutivos ao CMS (1 hora/dia, a 128mT, houve aumento em 38% na concentração de DHL (p<0.05, porém não houve mudanças nos índices hematimétricos, nas proteínas plasmáticas e nas transaminases. Duas semans após exposição ao CMS durante 30 dias consecutivos (CMS (1 hora/dia, a 128mT houve diminuição significativa das taxas de crescimento e aumento significativo das concetrações de proteínas (+62%, p<0.05, da hemoglobina (+10%, p<0.05, eritrócitos (+7%, p<0.05, leucócitos (+17%, p<0.05 e plaquetas (+10%, p<0.05. A exposição sub-crônica ao CMS induziu aumento da DHL (+43%, p<0.05, AST (+ 41%, p<0.05 e ALT (+95%, p<0.05. Em contraste não houve aumento da glicemia. Estas alterações sugerem que a exposição ao CMS possivelmente influencia a proliferação de células do sistema hematopoiético e a produção enzimática, indicando alterações teciduais.

  5. Effect of Dietary Administration of Lathyrus sativus Pulse on Intestinal Biochemical Parameters in Normal and Scorbutic Guinea Pigs

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective In order to investigate that ascorbic acid deficiency is responcible for lathyrus toxicity, the effect of dietary feeding of lathyrus pulse in normal and scorbutic guinea pigs for 3 months, on intestinal biochemical parameters was undertaken. Methods The intestinal brush border membrane (BBM) marker and xenobiotic metabolising enzymes (XME) were assayed. Results Exposure to 80% lathyrus alone and in scorbutic conditions showed significant inhibition of alkaline phosphatase (28%-30%), sucrase (19%) and γ-glutamyl transpeptidasc (GGT) (15%-27%)enzymes, while Ca2+-Mg2+-ATPase was significantly inhibited (38%) in scorbutic plus lathyrus treated group. The phase Ⅰ XME (AHH) remained unchanged while the phase Ⅱ enzyme glutathioneS-tranferase (GST) was significantly decreased (20%-22%) in lathyrus and scorbutic plus lathyrus treated groups. Quinone reductase (QR) activity was found to be significantly decreased in lathyrus exposed group (20%). The intestinal biomarker contents including hexose (25%-34%) and phospholipids (20%-40%) were significantly reduced in lathyrus and scorbutic plus lathyrus exposed animals, while sialic acid showed a significant decrease (28%) in scorbutic plus lathyrus treated group. However, cholesterol levels were signifcantly enhanced (15%-28%) in lathyrus and scorbutic plus lathyrus treated animals. Conclusion The results indicate that oral feeding of lathyrus pulse to guinea pigs can alter BBM parameters as well as XME, which may result in the intestinal toxicity.Further, ascorbic acid deficiency could be one of the pre-disposing factors of lathyrus toxicity.

  6. Relationships among bulk soil physicochemical, biochemical, and microbiological parameters in an organic alfalfa-rice rotation system.

    Science.gov (United States)

    Lopes, Ana R; Bello, Diana; Prieto-Fernández, Ángeles; Trasar-Cepeda, Carmen; Manaia, Célia M; Nunes, Olga C

    2015-08-01

    The microbial communities of bulk soil of rice paddy fields under an ancient organic agriculture regimen, consisting on an alfalfa-rice rotation system, were characterized. The drained soil of two adjacent paddies at different stages of the rotation was compared before rice seeding and after harvesting. The relationships among the soil microbial, physicochemical, and biochemical parameters were investigated using multivariate analyses. In the first year of rice cropping, aerobic cultivable heterotrophic populations correlated with lineages of presumably aerobic bacteria (e.g., Sphingobacteriales, Sphingomonadales). In the second year of rice cropping, the total C content correlated with presumable anaerobic bacteria (e.g., Anaerolineae). Independently of the year of rice cropping, before rice seeding, proteolytic activity correlated positively with the cultivable aerobic heterotrophic and ammonifier populations, the soil catabolic profile and with presumable aerobes (e.g., Sphingobacteriales, Rhizobiales) and anaerobes (e.g., Bacteroidales, Anaerolineae). After harvesting, strongest correlations were observed between cultivable diazotrophic populations and bacterial groups described as comprising N2 fixing members (e.g., Chloroflexi-Ellin6529, Betaproteobacteria, Alphaproteobacteria). It was demonstrated that chemical parameters and microbial functions were correlated with variations on the total bacterial community composition and structure occurring during rice cropping. A better understanding of these correlations and of their implications on soil productivity may be valid contributors for sustainable agriculture practices, based on ancient processes.

  7. Molecular and biochemical characterization of caffeine synthase and purine alkaloid concentration in guarana fruit.

    Science.gov (United States)

    Schimpl, Flávia Camila; Kiyota, Eduardo; Mayer, Juliana Lischka Sampaio; Gonçalves, José Francisco de Carvalho; da Silva, José Ferreira; Mazzafera, Paulo

    2014-09-01

    Guarana seeds have the highest caffeine concentration among plants accumulating purine alkaloids, but in contrast with coffee and tea, practically nothing is known about caffeine metabolism in this Amazonian plant. In this study, the levels of purine alkaloids in tissues of five guarana cultivars were determined. Theobromine was the main alkaloid that accumulated in leaves, stems, inflorescences and pericarps of fruit, while caffeine accumulated in the seeds and reached levels from 3.3% to 5.8%. In all tissues analysed, the alkaloid concentration, whether theobromine or caffeine, was higher in young/immature tissues, then decreasing with plant development/maturation. Caffeine synthase activity was highest in seeds of immature fruit. A nucleotide sequence (PcCS) was assembled with sequences retrieved from the EST database REALGENE using sequences of caffeine synthase from coffee and tea, whose expression was also highest in seeds from immature fruit. The PcCS has 1083bp and the protein sequence has greater similarity and identity with the caffeine synthase from cocoa (BTS1) and tea (TCS1). A recombinant PcCS allowed functional characterization of the enzyme as a bifunctional CS, able to catalyse the methylation of 7-methylxanthine to theobromine (3,7-dimethylxanthine), and theobromine to caffeine (1,3,7-trimethylxanthine), respectively. Among several substrates tested, PcCS showed higher affinity for theobromine, differing from all other caffeine synthases described so far, which have higher affinity for paraxanthine. When compared to previous knowledge on the protein structure of coffee caffeine synthase, the unique substrate affinity of PcCS is probably explained by the amino acid residues found in the active site of the predicted protein.

  8. Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus) experimentally infected with Trichinella zimbabwensis.

    Science.gov (United States)

    La Grange, Louis J; Mukaratirwa, Samson

    2014-08-21

    Fifteen crocodiles were randomly divided into three groups of five animals. They represented high-infection, medium-infection and low-infection groups of 642 larvae/kg, 414 larvae/kg and 134 larvae/kg bodyweight, respectively. The parameters assessed were blood glucose, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT). The humoral immune response to Trichinella zimbabwensis infection was evaluated in all three groups by an indirect ELISA method. The results showed deviations from normal parameters of blood glucose, CPK, LDH, AST and ALT when compared with reported levels in uninfected reptiles. Contrary to studies involving mammals, hypoglycaemia was not observed in the infected groups in this study. Peak values of blood glucose were reached on post-infection (PI) Day 49, Day 42 and Day 35 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of LDH and AST were observed on PI Day 56, Day 49 and Day 42 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of CPK were observed on Day 35 PI in all three groups. Peak ALT values were reached on Day 56 in the high-infection group and on Day 28 PI in both the medium-infection and low-infection groups. No correlations between the biochemical parameters and infection intensity were observed. Peak antibody titres were reached on Day 49 PI in the medium-infection group, and on Day 42 PI in both the high-infection and low-infection groups. Infection intensity could not be correlated with the magnitude of the humoral immune response or time to sero-conversion. Results from this study were in agreement with results reported in mammals infected with other Trichinella species and showed that antibody titres could not be detected indefinitely.

  9. Effect of soaked Moringa oleifera seeds on growth rates and the levels of some biochemical parameters in albino rats.

    Science.gov (United States)

    Igwilo, I O; Ogoke, T J; Ogbu, D O; Igwilo, S N; Obi, E; Abdulsalami, M S

    2013-01-01

    Moringa oleifera is a multipurpose tree, cultivated in the tropics and sub-tropics for its nutritional and therapeutic properties. The raw matured seeds which have been reported to be used as food and clarifying agent of turbid water caused growth retardation in albino rats and this might have been due to its antinutritional contents. Owing to these adverse factors, the effect of soaking the seeds for 30 min and then compounding it as feeds was done. Its effect on growth rate and the level of some biochemical parameters on rats were investigated. The Wistar albino rats were fed for 21 days and their weights measured at 2 days interval. Aspartate and Alanine transaminases, Alkaline phosphatase and total bilirubin levels were assayed using Automated Vitros 350 Chemistry Analyzer. The growth rates of rats fed with the commercial rat pellets, Casilan diet and the processed Moringa seed diet had a range of 80.06 +/- 3.54 to 100.98 +/- 5.37, 66.70 +/- 7.54 to 55.23 +/- 7.47 and 52.99 +/- 4.15 to 35.47 +/- 2.26, respectively. The parameters assayed for the group that received the processed Moringa seed diet are 144.00 +/- 16.80 (AST), 41.00 +/- 7.05 (ALT), 66.50 +/- 8.80 (ALP) and 12.45 +/- 1.18 (Total Bilirubin). The one-way ANOVA statistical analysis indicated that there was no significant change in the parameters of all the groups at 95% significance level (p Moringa oleifera seed did not support growth in the animal subjects and also did not pose a threat to the liver. However, it is better to develop more suitable processing methods to improve the seed's nutritional capabilities.

  10. Assessment of selected biochemical parameters and humoral immune response of Nile crocodiles (Crocodylus niloticus experimentally infected with Trichinella zimbabwensis

    Directory of Open Access Journals (Sweden)

    Louis J. La Grange

    2014-02-01

    Full Text Available Fifteen crocodiles were randomly divided into three groups of five animals. They represented high-infection, medium-infection and low-infection groups of 642 larvae/kg, 414 larvae/kg and 134 larvae/kg bodyweight, respectively. The parameters assessed were blood glucose, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, aspartate transaminase (AST and alanine transaminase (ALT. The humoral immune response to Trichinella zimbabwensis infection was evaluated in all three groups by an indirect ELISA method. The results showed deviations from normal parameters of blood glucose, CPK, LDH, AST and ALT when compared with reported levels in uninfected reptiles. Contrary to studies involving mammals, hypoglycaemia was not observed in the infected groups in this study. Peak values of blood glucose were reached on post-infection (PI Day 49, Day 42 and Day 35 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of LDH and AST were observed on PI Day 56, Day 49 and Day 42 in the high-infection, medium-infection and low-infection groups, respectively. Peak values of CPK were observed on Day 35 PI in all three groups. Peak ALT values were reached on Day 56 in the high-infection group and on Day 28 PI in both the medium-infection and low-infection groups. No correlations between the biochemical parameters and infection intensity were observed. Peak antibody titres were reached on Day 49 PI in the medium-infection group, and on Day 42 PI in both the high-infection and low-infection groups. Infection intensity could not be correlated with the magnitude of the humoral immune response or time to sero-conversion. Results from this study were in agreement with results reported in mammals infected with other Trichinella species and showed that antibody titres could not be detected indefinitely.

  11. Selected biochemical and oxidative stress parameters and ceruloplasmin as acute phase protein associated with bovine leukaemia virus infection in dairy cows

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    Akalın Pınar Peker

    2015-09-01

    Full Text Available The aim of this study was to determine the ceruloplasmin (Cp and vitamin C concentrations, the total antioxidant status (TAS, and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV. Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL, whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST (P = 0.003 and Cp concentrations (P = 0.03 decreased (65.17 ± 5.03 and 7.70 ± 0.72 respectively in BLV-infected cows, as compared to healthy animals (100.67 ± 11.50 and 10.40 ± 0.70 respectively. A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unknown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.

  12. Effect of vitamins, probiotics and protein level on semen traits and seminal plasma biochemical parameters of post-moult male broiler breeders.

    Science.gov (United States)

    Khan, R U; Rahman, Z U; Javed, I; Muhammad, F

    2013-01-01

    1. A study was designed to investigate the comparative effect of supplementary vitamin E, vitamin C, probiotics and dietary crude protein concentration on semen traits and seminal plasma biochemical parameters in male broiler breeders after Zn-induced moulting. 2. A total of 180 male broiler breeders were induced to moult at 65 weeks of age by mixing ZnO in diet at the rate 3000 mg/kg of feed. After moulting, the males were divided into six groups that were given feed supplemented with: vitamin C (500 IU/kg), vitamin E (100 IU/kg), protein (140 g CP/kg), probiotics (50 mg/kg) and the combination of these components, while one group was kept as a control. Semen samples were collected weekly and semen volume, spermatozoa concentration, motility and dead spermatozoa percentage were determined. Seminal plasma was separated to determine the concentration of total antioxidant capacity (TAC), homocysteine, paraoxonase (PON1), arylesterase, ceruloplasmin, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. 3. Semen volume was significantly higher in the vitamin E and C groups compared to the control. Spermatozoa motility was higher in the vitamin E group and dead spermatozoa percentage was significantly lower in the vitamin C group compared with the control group. 4. Seminal plasma TAC was higher in the vitamin E group, homocysteine was lower in the vitamin C and E groups. PON1 was higher in the combination group. Arylesterase increased significantly in the vitamin C and combination groups over time. Seminal plasma AST was significantly lower in the vitamin C and E supplemented groups whereas ALT decreased significantly only in the vitamin E group compared with the control. Higher concentrations of ceruloplasmin were observed in the combination group compared with the other treatments. 5. It was concluded that additional vitamin E and C or their combination was the most potent nutrient treatment for improving the semen traits and

  13. Thermodynamic parameters of the first order in low-concentration binary alloys

    Science.gov (United States)

    Bol'shov, L. A.; Korneichuk, S. K.

    2015-12-01

    Thermodynamic parameters of the first order (Wagner interaction parameter ɛ 2 (2) , enthalpy, and entropy parameter σ 2 (2) ) in low-concentration liquid binary alloys are considered. The values of these parameters for 32 binary systems are estimated from experimental data. A system of classification is proposed for the obtained data. These data are compared to similar data for aqueous solutions of nonelectrolytes. A qualitative explanation of the obtained differences is given.

  14. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue.

  15. Evaluation of hormonal change, biochemical parameters, and histopathological status of uterus in rats exposed to 50-Hz electromagnetic field.

    Science.gov (United States)

    Aydin, M; Cevik, A; Kandemir, F M; Yuksel, M; Apaydin, A M

    2009-04-01

    This study investigates the effects of the electromagnetic field (EMF) resulting from the 170 kV high-voltage power lines on hormonal status, on progesterone and 17-beta estradiol levels, and on morphology of the uterus and ovaries associated with biochemical parameters of adult Wistar female rats. The rats were assigned to experimental (21) and control groups (7). The rats in the experimental group were housed in a wooden barn with 7.5 m vertical distance to the power line. Groups 1, 2, and 3 were exposed continuously (24 h) to electric-electromagnetic fields (ELF-EMFs) (48.21 +/- 1.58 mG) for 1, 2, and 3 months, respectively. The rats of group 4 served as the control and were placed in laboratory conditions (The average value of the ELF was 0.75 +/- 0.05 V/m. The value of the EMF was calculated to be 0.48 +/- 0.05 mG.). Significant (P glutathione and malondialdehyde levels. It is concluded that exposure to the ELF-EMFs for different time periods produced significant decreases in plasma catalase activities in the 3-month exposure groups but no effects on progesterone level, on 17-beta estradiol level, or on the morphology and weight of uterus and ovaries.

  16. Influence of thyroid hormones on biochemical parameters of liver function: a case-control study in North Indian population

    Directory of Open Access Journals (Sweden)

    Jayashree Bhattacharjee

    2013-01-01

    Full Text Available Normal level of thyroid hormones is important for normal hepatic function and thyroid dysfunction may modulate metabolic function of liver. The purpose of this study is to determine whether liver function is associated with subclinical and overt hypothyroidism. Thyroid and liver function tests were evaluated in 47 patients with overt (TSH ≥10.0 mIU/L and 77 patients with subclinical hypothyroidism (TSH 6.0-9.9mIU/L and compared with 120 age-matched euthyroid controls. Subjects with overt hypothyroidism had significantly raised serum ALT, AST, ALP and total protein levels as compared to controls whereas subclinical hypothyroid patients had significantly increased levels of serum ALT, ALP and total protein. Further, TSH showed significant positive correlation with AST and ALP values whereas fT3 and fT4 had a negative correlation with AST in overt hypothyroidism. Thus, overt hypothyroid state is associated with significant derangement in biochemical parameters of liver function. Hence, liver function should be regularly monitored in hypothyroid patients.

  17. Association analysis of urotensin II gene (UTS2 and flanking regions with biochemical parameters related to insulin resistance.

    Directory of Open Access Journals (Sweden)

    María E Sáez

    Full Text Available BACKGROUND: Urotensin II (UII is a potent vasoconstrictor peptide, which signals through a G-protein coupled receptor (GPCR known as GPR14 or urotensin receptor (UTR. UII exerts a broad spectrum of actions in several systems such as vascular cell, heart muscle or pancreas, where it inhibits insulin release. OBJECTIVE: Given the reported role of UII in insulin secretion, we have performed a genetic association analysis of the UTS2 gene and flanking regions with biochemical parameters related to insulin resistance (fasting glucose, glucose 2 hours after a glucose overload, fasting insulin and insulin resistance estimated as HOMA. RESULTS AND CONCLUSIONS: We have identified several polymorphisms associated with the analysed clinical traits, not only at the UTS2 gene, but also in thePER3 gene, located upstream from UTS2. Our results are compatible with a role for UII in glucose homeostasis and diabetes although we cannot rule out the possibility that PER3 gene may underlie the reported associations.

  18. Effects of lycopene supplementation in both maternal and offspring diets on growth performance, antioxidant capacity and biochemical parameters in chicks.

    Science.gov (United States)

    Sun, B; Chen, C; Wang, W; Ma, J; Xie, Q; Gao, Y; Chen, F; Zhang, X; Bi, Y

    2015-02-01

    This study investigated the effects of different supplementation ways of lycopene during pre-hatch (from the diet of hens) and post-hatch (from the diet of progeny) on production performance, antioxidant capacity and biochemical parameters in chicks. In total, 360 hens were fed diets supplemented with 0 (control group) or 40 mg lycopene/kg diet. From 28 to 34 days after the start of supplementation (30 weeks old), 650 qualified eggs were collected to artificial incubation. In this trial, 2 × 2 factorial designs were used. Male chicks hatched from hens fed with 0 or 40 mg lycopene/kg diet were fed a diet containing either 0 or 40 mg lycopene/kg diet. The results showed that, relative to control, in ovo-deposited lycopene significantly increased chick birth body weight, improved liver total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and glutathione to oxidized glutathione ratio (GSH: GSSG), and significantly declined liver malondialdehyde (MDA) level and increased liver lycopene content during 0-14 days after hatching. On days 14 after hatching, dietary lycopene in diet began to take over gradually. Both supplementation ways of lycopene increased immune organ index, serum high-density lipoprotein (HDL) cholesterol, villus length and villus/crypt in duodenum, jejunum and ileum. Data in this study suggested lycopene supplementation could improve antioxidant capacity and immune function, and regulate lipid metabolism in chicks.

  19. Black pepper (Piper nigrum in diets for laying hens on performance, egg quality and blood biochemical parameters

    Directory of Open Access Journals (Sweden)

    Ramon Duque Melo

    2016-11-01

    Full Text Available The objective of this study was to evaluate the increasing levels (0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6% of black pepper in diets for laying hens on performance, egg quality and blood biochemical parameters. Hissex White hens (n=168 at 30 weeks of age were used. The experimental method was completely randomized with seven treatments with four replicates of six birds each. Estimates of black pepper levels were determined by polynomial regression. The performance showed no significant differences (p > 0.05. The eggshell percentage was significantly influenced (p < 0.05, in which the level of 0.30% inclusion impaired eggshell quality. Triglycerides level increased significantly (p < 0.05, according to increasing levels of black pepper in the diet. It can be concluded that black pepper can be used in diets for laying hens as phytogenic additive without harming the performance. However, this inclusion causes a reduction in eggshell percentage and an increase in the level of triglycerides in the bloodstream.

  20. Effect of adaptive threshold filtering on ultrasonic nakagami parameter to detect variation in scatterer concentration.

    Science.gov (United States)

    Tsui, Po-Hsiang; Wan, Yung-Liang; Huang, Chih-Chung; Wang, Ming-Chen

    2010-10-01

    The Nakagami parameter is associated with the Nakagami distribution estimated from ultrasonic backscattered signals and closely reflects the scatterer concentrations in tissues. There is an interest in exploring the possibility of enhancing the ability of the Nakagami parameter to characterize tissues. In this paper, we explore the effect of adaptive thresholdfiltering based on the noise-assisted empirical mode decomposition of the ultrasonic backscattered signals on the Nakagami parameter as a function of scatterer concentration for improving the Nakagami parameter performance. We carried out phantom experiments using 5 MHz focused and nonfocused transducers. Before filtering, the dynamic ranges of the Nakagami parameter, estimated using focused and nonfocused transducers between the scatterer concentrations of 2 and 32 scatterers/mm3, were 0.44 and 0.1, respectively. After filtering, the dynamic ranges of the Nakagami parameter, using the focused and nonfocused transducers, were 0.71 and 0.79, respectively. The experimental results showed that the adaptive threshold filter makes the Nakagami parameter measured by a focused transducer more sensitive to the variation in the scatterer concentration. The proposed method also endows the Nakagami parameter measured by a nonfocused transducer with the ability to differentiate various scatterer concentrations. However, the Nakagami parameters estimated by focused and nonfocused transducers after adaptive threshold filtering have different physical meanings: the former represents the statistics of signals backscattered from unresolvable scatterers while the latter is associated with stronger resolvable scatterers or local inhomogeneity due to scatterer aggregation.

  1. Changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run in relation to the age and speed of runners

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    Zbigniew Jastrzębski

    2016-10-01

    Full Text Available Objectives: The objective of the study was to reveal morphology, electrolyte and chosen biochemical parameters in terms of health risk in runners in reference to their age and running speed in the case of running a distance of 100 km, which occur after 12 h or 24 h of recovery. Material and Methods: Fourteen experienced, male, amateur, ultra-marathon runners, divided into two age and two speed groups took part in the 100-km run. Blood samples for analyses indexes were collected from the ulnar vein just before the run, after 25 km, 50 km, 75 km and 100 km, as well as 12 h and 24 h after termination of the run. Results: The sustained ultramarathon run along with the distance covered (p < 0.05 caused an increase in myoglobin (max 90-fold, bilirubin (max 2.8-fold and total antioxidant status (max 1.15-fold, which also continued during the recovery. Significant changes in the number of white blood cells were observed with each sequential course and could be associated with muscle damage. The electrolyte showed changes towards slight hyperkalemia, but no changes in natrium and calcium concentrations. There were no significant differences between the age and speed groups for all the parameters after completing the 100-km run as well as after 12 h and 24 h of recovery. Conclusions: Considering changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run it can be stated that such an exhausting effort may be dangerous for human health due to metabolic changes and large damage to the organs. Negative metabolic changes are independent of age of an ultramarathon runner and occur both in younger (32±5.33 years and older participants (50.56±9.7 years. It can be concluded that organ damage and negative metabolic changes during a 100-km run occur similarly in participants less experienced as well as in well trained runners. Int J Occup Med Environ Health 2016;29(5:801–814

  2. Changes in Biochemical, Strength, Flexibility, and Aerobic Capacity Parameters after a 1700 km Ultraendurance Cycling Race

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    Vicente Javier Clemente-Suarez

    2014-01-01

    Full Text Available The purpose of the present research was to study the organic response after ultraendurance cycling race. Selected biochemical, leg strength, flexibility, and aerobic capacity parameters were analyzed in 6 subjects 5 days before and 5 days after completing a 1700 km ultraendurance cycling race. After the race, participants presented a significant decrease in Hb (167.8 ± 9.5 versus 141.6 ± 15.7 mg/dL, strength (29.4 ± 2.7 versus 25.5 ± 3.7 cm in a countermovement jump, and oxygen uptake and heart rate at ventilatory threshold (1957.0 ± 458.4 versus 1755.2 ± 281.5 mL/kg/min and 140.0 ± 9.7 versus 130.8 ± 8.3 bpm, resp.. Testosterone presented a decrease tendency (4.2 ± 2.5 versus 3.9 ± 2.6 ng/L in opposition to the increase tendency of cortisol and ammonium parameters. Transferrin and iron levels presented high values related to an overstimulation of the liver, a normal renal function, a tendency to decrease flexibility, and an increase in aerobic capacity, finding a tendency to increase the absolute maximal oxygen uptake (37.2 ±2.4 versus 38.7 ± 1.8 mL/min in contrast to previous studies conducted with subjects with similar age. These results can be used to program training interventions, recovery times between probes, and nutritional and/or ergonomic strategies in ultraendurance events.

  3. Effect of Pesticide Exposure on Immunological, Hematological and Biochemical Parameters in Thai Orchid Farmers— A Cross-Sectional Study

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    Soraya Aroonvilairat

    2015-05-01

    Full Text Available Various studies have found that many Thai orchid farmers used excessive amounts of pesticides without proper protective gear, but no toxicological study has been made. This cross-sectional study aimed to evaluate the immunological, hematological and biochemical statuses of these farmers. Sixty four orchid farmers and 60 controls were studied. Plasma cholinesterase activity, the percentage and absolute number of B lymphocytes (CD19+ were significantly lower in the farmers group (3966.32 ± 1165.48 U/L, 11.61 ± 4.09% and 312.26 ± 164.83 cells/mm3, respectively as compared to those of controls (5048.85 ± 1139.40 U/L, 14.32 ± 4.23%, 420.34 ± 195.18 cells/mm3, respectively. There was a statistically significant higher level of serum IgE among the orchid farmers (0.031 ± 0.011 mg/dL vs. 0.018 ± 0.007 mg/dL but not IgG, IgA and IgM, levels. Serum lysozyme level, lymphocyte proliferative responses to mitogens, hematological parameters and kidney function test, were not significantly different between the two groups. The liver function profiles showed significantly lower levels of albumin and serum protein in the farmer group. Thus frequent pesticide exposure resulted in subtle changes of some biological parameters. These changes, though may not be clinically significant, strongly indicated that caution in handing pesticides by these farmers is warranted.

  4. Effect of pesticide exposure on immunological, hematological and biochemical parameters in thai orchid farmers- a cross-sectional study.

    Science.gov (United States)

    Aroonvilairat, Soraya; Kespichayawattana, Wannapa; Sornprachum, Thiwaree; Chaisuriya, Papada; Siwadune, Taweeratana; Ratanabanangkoon, Kavi

    2015-05-27

    Various studies have found that many Thai orchid farmers used excessive amounts of pesticides without proper protective gear, but no toxicological study has been made. This cross-sectional study aimed to evaluate the immunological, hematological and biochemical statuses of these farmers. Sixty four orchid farmers and 60 controls were studied. Plasma cholinesterase activity, the percentage and absolute number of B lymphocytes (CD19+) were significantly lower in the farmers group (3966.32±1165.48 U/L, 11.61±4.09% and 312.26±164.83 cells/mm3, respectively) as compared to those of controls (5048.85±1139.40 U/L, 14.32±4.23%, 420.34±195.18 cells/mm3, respectively). There was a statistically significant higher level of serum IgE among the orchid farmers (0.031±0.011 mg/dL vs. 0.018±0.007 mg/dL) but not IgG, IgA and IgM, levels. Serum lysozyme level, lymphocyte proliferative responses to mitogens, hematological parameters and kidney function test, were not significantly different between the two groups. The liver function profiles showed significantly lower levels of albumin and serum protein in the farmer group. Thus frequent pesticide exposure resulted in subtle changes of some biological parameters. These changes, though may not be clinically significant, strongly indicated that caution in handing pesticides by these farmers is warranted.

  5. Effect of Pesticide Exposure on Immunological, Hematological and Biochemical Parameters in Thai Orchid Farmers—A Cross-Sectional Study

    Science.gov (United States)

    Aroonvilairat, Soraya; Kespichayawattana, Wannapa; Sornprachum, Thiwaree; Chaisuriya, Papada; Siwadune, Taweeratana; Ratanabanangkoon, Kavi

    2015-01-01

    Various studies have found that many Thai orchid farmers used excessive amounts of pesticides without proper protective gear, but no toxicological study has been made. This cross-sectional study aimed to evaluate the immunological, hematological and biochemical statuses of these farmers. Sixty four orchid farmers and 60 controls were studied. Plasma cholinesterase activity, the percentage and absolute number of B lymphocytes (CD19+) were significantly lower in the farmers group (3966.32 ± 1165.48 U/L, 11.61 ± 4.09% and 312.26 ± 164.83 cells/mm3, respectively) as compared to those of controls (5048.85 ± 1139.40 U/L, 14.32 ± 4.23%, 420.34 ± 195.18 cells/mm3, respectively). There was a statistically significant higher level of serum IgE among the orchid farmers (0.031 ± 0.011 mg/dL vs. 0.018 ± 0.007 mg/dL) but not IgG, IgA and IgM, levels. Serum lysozyme level, lymphocyte proliferative responses to mitogens, hematological parameters and kidney function test, were not significantly different between the two groups. The liver function profiles showed significantly lower levels of albumin and serum protein in the farmer group. Thus frequent pesticide exposure resulted in subtle changes of some biological parameters. These changes, though may not be clinically significant, strongly indicated that caution in handing pesticides by these farmers is warranted. PMID:26024358

  6. Some biochemical parameters of blood plasma of turkey-hens following administration of 1,2,4-triasole derivative.

    Science.gov (United States)

    Krauze, M; Truchliński, J; Cendrowska-Pinkosz, M

    2007-01-01

    The present study involved 180 slaughter turkey-hens of heavy Big-6 type divided into four groups (in triplicate repetition for 15 birds). All the birds were fed with the same standard full-dose mixtures in 5-stage system. The turkey-hens of groups I, II and III were given 1,2,4-triasole derivative (3-(2-pyridil)-4-phenyl-1,2,4-triasole-5-carboxylic acid), which has antibacterial, antifungal and immunomodulating properties, in amount of 50, 75 and 100 microg per 1 dm3 of water. Group IV--control was given water without the additive. The 1,2,4-triasole derivative was given to drinking water, starting from the first day of bird's life and for the whole rearing period. The present results of biochemical analysis of blood plasma showed that addition of examined substance significantly reduced concentration of protein, glucose, triglycerides and uric acid as compared to control. It was stated that tested 1,2,4-triasole derivative elevated the level of HDL fraction percentage and alkaline phosphatase activity in blood plasma.

  7. Effect of vitamin e supplementation on biochemical parameters in pesticides sprayers of grape gardens of Western maharashtra (India).

    Science.gov (United States)

    Patil, Jyotsna A; Patil, Arun J; Sontakke, Ajit V; Govindwar, Sanjay P

    2012-04-01

    The aim of this study was to see the biochemical effects of pesticides on sprayers of grape gardens before and after 15 days of vitamin E supplementations in Western Maharashtra (India), who were occupationally exposed to various pesticides over a long period of time (about 5 to 15 years). Blood samples were collected from all study group subjects for biochemical parameters assays before and after 15 days of vitamin E supplementation. Sprayers of grape gardens were given 400 mg of vitamin E tablet/day for 15 days. After 15 days of vitamin E supplementation to sprayers of grape gardens, we observed significantly decreased aspartate transaminase (10.88 %, P gardens, who received vitamin E supplementation, showed significantly decreased serum lipid peroxide (LP) (18.75 %, P < 0.001, r = 0.63) and significantly increased RBC-superoxide dismutase (SOD) (12.88 %, P < 0.001, r = 0.85), RBC-Catalase (CAT) (24.49 %, P < 0.001, r = 0.70), plasma ceruloplasmin (CP) (4.6 %, P < 0.01, r = 0.80), serum zinc (4.57 %, P < 0.01, r = 0.83) and serum copper (4.37 %, P < 0.01, r = 0.79) as compared to values before vitamin E supplementation. These results showed that vitamin E supplementation has ameliorating effects on these transaminase enzymes, suggesting that it may have a protective effect on liver, from pesticides induced damage. In this study vitamin E supplementation might have decreased LP levels by breaking chain reaction of lipid peroxidation. Present results indicate that vitamin E plays a crucial role in restoring the antioxidant enzymes such as SOD, CAT and CP, in population exposed to pesticides. This helps to enhance its antioxidant ability. Therefore, it is suggested that farmers, pesticide applicators, workers in the pesticide industry and other pesticide users, who come in regular contact with pesticides, may be benefited by supplementation with vitamin E.

  8. Association of Helicobacter pylori IgG antibody with various demographic and biochemical parameters in kidney transplant recipients

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    Azar Baradaran

    2011-01-01

    Full Text Available Few reports are available regarding the promoting factors that affect Helicobacter pylori (H. pylori infection in renal transplant (RTx patients. We report a cross-sectional study that was conducted on a group of stable RTx patients to investigate the relationship of various demographic and biochemical parameters of these patients with serum H. pylori IgG antibody titer as a sign of H. pylori infection. A total of 72 patients who were referred to the clinic for continuing their treatment were enrolled in this study. These patients included 47 males and 25 females. The mean age of the study patients was 44 (±12 years. The mean length of time after they received a transplanted kidney was 67.5 (±42 months (median: 62 months. The mean value of serum H. pylori-specific IgG antibody titer among these patients was 3 (±4.6 U/mL (median: 1 U/mL, and that of intact parathormone (iPTH was 18.4 (±8.2 pg/mL (median: 16.5 pg/mL. The mean serum magnesium (Mg was 1.9 (±0.20 mg/dL (median: 1.9 mg/dL and the mean creatinine clearance was 53 (±11 mL/min (median: 56 mL/min. In this study population, there was no significant difference in the H. pylori IgG antibody titers, serum iPTH, Mg, calcium, alkaline phosphatase and albumin levels as well as body mass index (BMI between males and females or diabetics and non-diabetics. There was no significant relationship between serum H. pylori IgG antibody titers and the age of the patients, BMI, serum Alb, phosphorus, Ca, serum leptin and serum ALP. Significant negative correlation between serum H. pylori IgG antibody titers and serum Mg (r = -0.30, P = 0.01 and serum iPTH (r = -0.25, P = 0.03 was seen. A significant positive correlation was found between serum H. pylori IgG antibody titer and creatinine clearance (r = 0.26, P = 0.02, and a near-significant positive correlation was found with the duration of RTx (r = 0.20, P = 0.08. Our study shows that the correlation of H. pylori IgG antibody titer with some

  9. Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and Leporinus obtusidens (Valenciennes, 1837

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    Vania Lucia Loro

    Full Text Available Rhamdia quelen (silver catfish and Leporinus obtusidens (piava were exposed to a commercial formulation Roundup(r, a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT and aspartate aminotransferase (AST activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase activities. Plasmatic glucose levels were reduced in both species, whereas the transaminase activities (ALT and AST increased after exposure to the herbicide. Herbicide exposure increased protein and glucose levels in the mucus layer in both species. There was a reduction in both NTPDase and ecto-5'-nucleotidase activity in the brain of piava, and increased enzyme activity in silver catfish at both concentrations tested. The species showed an increase in protein carbonyl in the liver after exposure to both concentrations of the glyphosate. Our results demonstrated that exposure to Roundup(r caused liver damage, as evidenced by increased plasma transaminases and liver protein carbonyl in both of the fish species studied. The mucus composition changed and hypoglycemia was detected after Roundup(r exposure in both species. Brain nucleotide hydrolysis showed a different response for each fish species studied. These parameters indicated some important and potential indicators of glyphosate contamination in aquatic ecosystems.

  10. Effects of supplementing rare earth element cerium on rumen fermentation, nutrient digestibility, nitrogen balance and plasma biochemical parameters in beef cattle.

    Science.gov (United States)

    Lin, S X; Wei, C; Zhao, G Y; Zhang, T T; Yang, K

    2015-12-01

    The objectives of the trial were to investigate the effects of supplementing rare earth element (REE) cerium (Ce) on rumen fermentation, nutrient digestibility, methane (CH4 ) production, nitrogen (N) balance and plasma biochemical parameters in beef cattle. Four Simmental male cattle, aged at 14 months, with initial liveweight of 355 ± 8 kg and fitted with permanent rumen cannulas, were used as experimental animals. The cattle were fed with a total mixed ration (TMR) composed of concentrate mixture and corn silage. Four levels of cerium chloride (CeCl3 ·7H2 O, purity 99.9%), that is 0, 80, 160 and 240 mg CeCl3 /kg DM, were added to basal ration in a 4 × 4 Latin square design. Each experimental period lasted 15 days, of which the first 12 days were for pre-treatment and the last 3 days were for sampling. The results showed that supplementing CeCl3 at 160 or 240 mg/kg DM increased neutral detergent fibre (NDF) digestibility (p cattle increased the digestibility of NDF, decreased the molar ratio of rumen acetate to propionate, increased N retention and microbial N flow and decreased CH4 /kg DMI.

  11. Effects of aqueous and ethanol extract of dried leaves of Pseudocalymma alliaceum (Bignonaceae) on haematological and biochemical parameters of wistar rats

    Institute of Scientific and Technical Information of China (English)

    Carlos Granados-Echegoyen; Rafael Prez-Pacheco; Alfonso Alexander-Aguilera; Luicita Lagunez-Rivera; Nancy Alonso-Hernndez

    2015-01-01

    Objective: To perform the toxicological evaluation of aqueous and ethanol extract of dried leaves of Pseudocalymma alliaceum (P. alliaceum) in male Wistar rats by oral administration for 14 days, and to determine the biochemical and haematological status of blood. Methods: The animals were completely randomized into four groups of three rats each. Results: No deaths were reported after oral administration of the extracts, no physical signs of toxicity or adverse effects were observed. Hematological indices of red blood cell count, hemoglobin concentration and mean corpuscular hemoglobin showed no significant abnormality; however, white series levels decrease presenting a leukopenia. Glucose, creatinine and albumin increased, while urea decreased; aspartate aminotransferase values decreased with the aqueous extract at 50 and 100 mg/kg and increased with dose of 200 mg/kg, in contrast ethanol extract caused an increase in this parameter to the doses used. The alanine aminotransferase decreased with aqueous extract and increased with ethanol extract. Triglycerides decreased when used aqueous extract and reduced with ethanol extract at 100 and 200 mg/kg, in contrast to 50 mg/kg decreased to be compared with control group. Conclusion: The daily intake of P. alliaceum did not produce acute toxicity to 50 mg/kg which may be interpreted as toxic signs or biological damage, but liver and renal function changes at dosages of 100 and 200 mg/kg; however, the reduction ability of white blood cells count could be used as a basis for specific studies on the treatment of patients with leukemia.

  12. EFFECTS OF SOYBEAN MEAL BASED DIET ON GROWTH PERFORMANCE AND HEMOLYMPH BIOCHEMICAL PARAMETERS OF NARROW-CLAWED CRAYFISH (ASTACUS LEPTODACYLUS ESCHSCHOLTZ, 1823

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    Mahdi Banaee

    2013-01-01

    Full Text Available Like other crustaceans, narrow-clawed crayfish (Astacus leptodacylus Eschscholtz, 1823 can change its diet to the available food during varied life cycles. Diet alteration can affect different biological indices of this species, therefore this study aims at studying changes in growth indices, hemolymph biochemical parameters and biochemical quality of its carcasses, which might occur during diet change of crayfish. The purpose of this experiment was to evaluate the effects of partial replacement of fish meal with soybean meal on growth performance, carcass quality and hemolymph biochemical parameters of narrow-clawed crayfish. 90 healthy adult narrow-clawed crayfish (W=35.50±4.05 g; TL=16.96±1.92 cm were randomly distributed into 9 fiberglass tanks (200 L and were fed for 45 days with three varied diets including: commercial shrimp diet, fishmeal-based diet (A and soybean meal-based diet (B. The results show that changing the diet from animal protein to plant protein caused a significant decrease in the cholesterol and triglyceride levels in hemolymph, as well as carcass fat of the crayfish nourished with B diet when compared with the crayfish fed with A diet. No significant changes of hemolymph levels of glucose, AST and ALT were found between different treatments during this experimental period. In conclusion, it was found that though growth performance reduced, the increased rate of soybean meal in diet from 0.0% to 76% had no adverse effects on biochemical parameters.

  13. Age-Dependent Variation in Hormonal Concentration and Biochemical Constituents in Blood Plasma of Indian Native Fowl

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    Avishek Biswas

    2010-01-01

    Full Text Available This experiment was to investigate the age-related changes in hormonal concentration and biochemical constituents of blood plasma in Indian native desi fowl. One hundred and sixty two (54 from each breed, i.e., Kadaknath (KN, Aseel peela (AP, and White leghorn (WLH day-old female chicks were randomly divided into nine groups each of 18 chicks (3 groups × 3 replicates. WLH was taken in this study to compare the characteristics of Indian native desi fowl. The highest level of estrogen hormone in WLH and desi fowl in blood plasma was occurred at 18 and 24 wks of age, respectively. Whereas, the peak of progesterone hormone in WLH hens noticed around 24 wks, in case of desi fowls, it was at 30 wks of age. Irrespective of the breed, the hormonal profile of Triiodothyronine (T3 and Thyroxine (T4 in blood plasma was found highest around 6 to 12 wks of age. Activities of acid phosphatase (ACP increased with the reduction of alkaline phosphate (ALP activities at different time intervals. Irrespective of the breed, transaminases (glutamic oxaloacetic transaminase (GOT and glutamic pyruvic transaminase (GPT activities of blood plasma increased linearly with the advancement of the age. From this study, it may be concluded that sexual maturity of the Indian native desi fowl occurred nearly 6 wk later (24 wk than WLH.

  14. Impact of Testosterone, Zinc, Calcium and Magnesium Concentrations on Sperm Parameters in Subfertile Men

    Science.gov (United States)

    Aydemir, Birsen; Kiziler, Ali Riza; Onaran, Ilhan; Alici, Bülent; Özkara, Hamdi; Akyolcu, Mehmet Can

    2007-04-01

    To investigate the impact of testosterone, zinc, calcium and magnesium concentrations in serum and seminal plasma on sperm parameters. There were significant decrease in sperm parameters, serum and seminal plasma zinc levels in subfertile males. It indicates zinc has a essential role in male infertility; the determination the level of zinc during infertility investigation is recommended.

  15. Effect of Ethanolic Extract of Emblica officinalis on Histopathology of Kidney and on Biochemical Parameters in Hyperlipidemic Albino Rats

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    Bheemshetty S. Patil

    2015-01-01

    Full Text Available Background: It has been reported that hyperlipidemia plays a central role in the development of atherosclerosis and oxidative stress. Embilica officinalis also known as Amla or Indian Gooseberry acts as antihyperlipidemic and antioxidant. Its active ingredients contains tannins, gallic acid and flavonoids. Aim & Objectives: It was aimed to evaluate the effect of ethanolic extract of Emblica officinalis on histopathology of kidney and on biochemical parameters in hyperlipidemic albino Wistar rats. Material and Methods: Extraction of dried fruits of Emblica officinalis was done by Soxhlet apparatus 0 using 99% ethanol at 60 C for 24 hours and also phytochemical analysis was done. Group I served as normal control. Group II was fed with isocaloric diet. Group III was fed with hyperlipidemic diet. Group IV was fed with isocaloric diet for 21 days + Embilica officinalis for 21 days. Group V was fed with hyperlipidemic diet for 21 days+ Embilica officinalis for 21 days. The dose of ethanolic extract of Emblica officinalis was taken as 100mg/kg body weight daily. Results: Percent body weight gain, kidney weight and nephro-somatic index significantly improved in hyperlipidemic rats treated with Emblica officinalis. There was a significant improvement in serum electrolyte and kidney markers. It was found that there were focal glomerular lesions with thickening of glomerulus in the kidneys of rats on hyperlipidemic diet and normal renal histology of rats on hyperlipidemic diet treated with Emblica officinalis. Conclusion: It can be concluded that Emblica officinalis may be a good, natural therapeutic agent against hyperlipidemic diet induced oxidative damage and nephrotoxicity.

  16. Estimation of the action of three different mechlorethamine doses on biochemical parameters during experimentally induced pleuritis in rats.

    Science.gov (United States)

    Całkosiński, Ireneusz; Rosińczuk-Tonderys, Joanna; Dzierzba, Katarzyna; Bronowicka-Szydełko, Agnieszka; Seweryn, Ewa; Majda, Jacek; Całkosińska, Monika; Gamian, Andrzej

    2011-01-01

    Nitrogranulogen (NTG) may modify the character of inflammatory reactions. These modifications are a result of cytotoxic and mutagenic effects. NTG has high affinity to DNA and causes disorders in the synthesis of acute phase proteins (e.g., haptoglobin, transferrin, fibrinogen, and complement protein C3). Our previous studies have shown that small doses of NTG can enhance immunological defense reactions in the organism. The aim of the current studies was to determine how different NTG doses cause changes in the values of biochemical parameters in pleuritis-induced rats. The animals were randomized into five groups: Group I - control group; Group II - IP (induced pleuritis) group; Group III - NTG5 group; Group IV - NTG50 group; Group V - NTG600 group. Blood was collected from all groups of animals at 24, 48, and 72 h after the initiation of the carrageenin-induced inflammatory reaction. These investigations revealed that a dose of 5 μg NTG/kg b.w. (body weight) can change the character of the inflammation. Our studies also show that a dose of 600 μg NTG/kg b.w. causes a rapid decrease in the level of C3 at the 72 h of the experiment (after 3 applications every 24 h), which indicates a cytotoxic action of such a large NTG dose. NTG used at doses of 50 and 600 μg/kg b.w. causes the opposite metabolism of albumins and other serum proteins. Our studies show that the different doses of NTG have distinct effects on the inflammatory reaction.

  17. Detection of vitamin B12 levels with the aid of some hematological and biochemical parameters that are more sensitive

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    Süheyl Asma

    2010-09-01

    Full Text Available Vitamin B12 deficiency is a health problem, which is usually seen in elderly patients and results in hematological and neurological disorders. In this study, we aimed to investigate the serum levels of vitamin B12 could represent the real tissue levels with con-sidering some hematological and biochemical parameters. 128 patients whose vitamin B12 levels have been detected to be low and their ages are ranged from 16 to 90, have been investigated. Fifty three of them were females. Complete blood counts, antiparietal antibody(APA, homocysteine levels, peripheral blood smear evaluations and findings of gastroduodenoscopy were assessed. Mean volume of erythrocytes has been found as 107 fl in patient group (group 1, in which vitamin B12 level has been detected lower than 60 pg/mL. Thehigher homocysteine values in 85% cases, neutrophil hyper-segmentation in 82%, atrophic gastritis in 53 % and APA positivity in 47 % of cases have been detected. The average of agehas been detected as 65 in this group. High level of homocysteine has been present in 44 % of cases and MCV has been 91 fl in group 2, in which the vitamin B12 levels are between 61–100 pg/ mL. In group ( group 3, in which vitamin levels are between 101 – 140 pg /mL,elevated homocysteine levels in 27 % cases and MCV as 89 fl have been detected. In group 4, in which vitamin levels are between 141–178 pg/mL, homocysteine elevation in 16 % of cases and MCV as

  18. Effects of aqueous extract of Withania somnifera on some liver biochemical and histopathological parameters in male guinea pigs.

    Science.gov (United States)

    Al-Awthan, Yahya S; Hezabr, Samira M; Al-Zubairi, Aisha M; Al-Hemiri, Faten A

    2014-04-01

    Organophosphorus (OP) insecticides constitute one of the most widely used classes of pesticides being employed for both agricultural and landscape pest control. The purpose of this study was to investigate the effect of dimethoate (DM), an organophosphorus insecticide, on some biochemical and histopathological parameters in liver of adult male guinea pigs as well as the possible role of Withania somnifera extract in attenuation of DM-induced hepatotoxicity. The animals were divided randomly into 5 groups and kept at 5 animals per group in an environmentally controlled condition with free access to food and water ad libitum. The first group was served as a control group and administered with olive oil orally; the group II received aqueous extract of W. somnifera (100 mg kg(-1) b.wt.) orally, group III, IV and V was administered with DM (14 mg kg(-1); 1/25LD50) for 21 days orally. Group IV and V received 100 mg kg(-1) of W. somnifera extract and silymarin, respectively half hour before DM administration for 21 days. DM caused a statistically significant increase in the serum level of liver enzymes (AST, ALT, ALP) when compared to control animals, whereas, W. somnifera and silymarin pre-treatment to the DM-intoxicated animals resulted in a significant normalization of the enzymes activities. On the other hand W. somnifera extract reduced the incidence of histopathological changes such as cytoplasmic vacuolization and degeneration in nuclei, rupture of epithelia lining the central vein, widened sinusoidal space and lymphocyte infiltration induced by DM treatment in guinea pigs. In conclusion, the results of this study suggest that W. somnifera aqueous extract could protect the liver against DM-induced oxidative damage.

  19. Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats.

    Science.gov (United States)

    Cano Barquilla, Pilar; Pagano, Eleonora S; Jiménez-Ortega, Vanesa; Fernández-Mateos, Pilar; Esquifino, Ana I; Cardinali, Daniel P

    2014-10-01

    The objective of this study was to evaluate the efficacy of melatonin to affect mild inflammation in the metabolic syndrome (MS) induced by a high-fat diet in rats. Adult Wistar male rats were divided into four groups (n = 16/group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet + melatonin; and (iv) melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions for 10 wk: (a) tap water; (b) 25 μg/mL of melatonin. Plasma interleukin (IL)-1β, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and C-reactive protein (CRP) were measured at two time intervals, that is, the middle of daylight period and the middle of the scotophase. In addition, a number of somatic and metabolic components employed clinically to monitor the MS were measured. Melatonin decreased the augmented circulating levels of IL-1β, IL-6, TNF-α, IFN-γ, and CRP seen in obese rats and restored the depressed levels of IL-4 and IL-10. Rats fed with the high-fat diet showed significantly higher body weights and augmented systolic blood pressure from the third and fourth week onwards, respectively, melatonin effectively preventing these changes. In high-fat-fed rats, circulating low-density lipoprotein-cholesterol, total cholesterol, and triglyceride concentration augmented significantly, melatonin being effective to counteract these changes. Melatonin-treated rats showed a decreased insulin resistance, the highest values of plasma high-density lipoprotein-cholesterol, and the lowest values of plasma uric acid. The results indicate that melatonin is able to normalize the altered biochemical pro-inflammatory profile seen in rats fed with a high-fat diet.

  20. Comparative toxicity of physiological and biochemical parameters in Euglena gracilis to short-term exposure to potassium sorbate.

    Science.gov (United States)

    Engel, Fernanda; Pinto, Luciano Henrique; Del Ciampo, Lineu Fernando; Lorenzi, Luciano; Heyder, Carmen Diamantina Teixeira; Häder, Donat Peter; Erzinger, Gilmar Sidnei

    2015-01-01

    Potassium sorbate is the potassium salt of sorbic acid, is a widespread and efficient antioxidant that has multiple functions in plants, traditionally associated with the reactions of photosynthesis; however, it has moderate toxicity to various species including rat, fish, bacteria and human health. The effects of potassium sorbate on the movement and photosynthetic parameters of Euglena gracilis were studied during short-term exposure. Potassium sorbate showed acute toxicity to the green flagellate E. gracilis affecting different physiological parameters used as endpoints in an automatic bioassay such as motility, precision of gravitational orientation (r-value), upward movement and alignment, with mean EC50 values of 2867.2 mg L(-1). The concentrations above 625 mg L(-1) of potassium sorbate induce an inhibition of the photosynthetic efficiency and electron transport rate and, in concentrations more than 2500.0 mg L(-1), the Euglena cells undergo a complete inhibition of photosynthesis even at low light irradiation.

  1. A novel method for measuring the concentration of chloroform based on kinetic parameters at atmosphere

    Science.gov (United States)

    Wang, Yuguo; Han, Haiyan; Chang, Tao; Liu, Xiuhong; Zhu, Qiaofen; Liu, Feng; Yan, Yongliang; Shen, Chengyin; Chu, Yannan

    2016-06-01

    A novel method is proposed to detect chloroform concentrations based on the kinetic parameters using ion mobility spectrometer with a negative corona discharge ion source operating at atmospheric pressure. Unlike conventional sample introduction mode, in this technique, CHCl3 enters into the drift tube from the end of drift region carried by the drift gas. There are two tails before Cl- and (CHCl3)·Cl- ion peaks, which fit to the ions formed in the drift region. Utilizing the kinetic parameters, concentration for CHCl3 can be calculated. This method not only offers a new way to get concentrations of CHCl3 under atmospheric pressure.

  2. Hematology and Some Biochemical Parameters of Wild Rodents in Pistachio Gardens of Kerman Province,Southeast Iran%Hematology and Some Biochemical Parameters of Wild Rodents in Pistachio Gardens of Kerman Province, Southeast Iran

    Institute of Scientific and Technical Information of China (English)

    MADJDZADEH SM; ABBASNEJAD M; TAKALLOOZADEH HM

    2011-01-01

    Wild rodents were collected using live snap traps in pistachio gardens of Kerman Province,Southeast Iran from 2007 to 2009,then some physiological parameters of them were measured.The samples were identified as follow:Nesokia indica,Meriones persicus,Meriones lybicus and Tatera indica.Blood samples were obtained from the heart,then the blood parameters (glucose,cholesterol,triglyceride,total protein,HDL,red and white blood cell number) in wild species of rodents and laboratory rat were compared.The results showed that there were no significant differences in serum glucose,triglyceride,HDL and total protein levels among different experimental groups.The concentration of cholesterol in T.indica was more than that in N.indica (P < 0.01).The total numbers of red blood cells also showed significant difference between wild garden rodent species and laboratory rat (P < 0.01),while the numbers of white blood cells showed no significant difference.

  3. Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae

    Directory of Open Access Journals (Sweden)

    Juliana Castro Monteiro

    2009-04-01

    Full Text Available Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT, alanine transaminase (ALT, aspartate transaminase (AST, chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG occurred in the group that received the higher dose.As folhas de Rudgea viburnoides Benth. são utilizadas na medicina popular como diuréticas, hipotensoras, anti-reumáticas, depurativas do sangue e em regimes de emagrecimento. O presente estudo foi delineado para avaliar o efeito da infusão das folhas de R. viburnoides nos parâmetros bioquímicos plasmáticos e na morfologia e morfometria hepática de ratos Wistar adultos. Dois grupos receberam a infusão das folhas, diariamente, nas dosagens de 10 e 20 g de folhas secas/L de água, durante 40 dias. O grupo controle recebeu a mesma quantidade de água. As análises histopatológicas não mostraram áreas degeneradas e infiltrados inflamatórios. A morfometria hepática mostrou acúmulo significativo de gordura nos hepatócitos dos animais tratados, principalmente no grupo que recebeu a maior dose da infusão (8,75% de gotículas lipídicas, comparado com 0,25% delas encontradas nos animais controles. Não foram observadas alterações nos níveis plasmáticos de uréia, creatinina,

  4. Comparison of some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred race horses

    Directory of Open Access Journals (Sweden)

    Bilal Tarik

    2004-01-01

    Full Text Available The aim of this study was to determine some blood parameters, serum vitamin E and mineral concentrations of Arabian and English thoroughbred racehorses fed the same diets. The diet was formulated to provide 2.31 Mcal DE/kg, and 10.96% crude protein. Total protein, lactate, calcium, phosphorus, potassium copper, cobalt and zinc were determined in serum obtained from 40 Arabian and 40 English healthy racing thoroughbred horses aged 2-3. The copper, cobalt and zinc concentrations were determined by atomic absorption, vitamin E by HPLC and the other biochemical parameters by a spectrophotometer. Mean values were 6.77 and 6.86 g/dl for total protein, 1.88 and 2.16 mg/dl for lactate 13.18 and 12.80 mg/dl for calcium, 4.35 and 4.39 mmol/l for phosphorus, 2.64 and 3.14 mmol/l for potassium, 129 and 166 μg/dl for copper, 36 and 44 μg/dl for cobalt and, 160 and 58 μg/dl for zinc in Arabian and English horses respectively, and Mean serum vitamin E levels were 2.65 and 2.81 μg/ml respectively. This study did not demonstrate a significant effect of breed on serum total protein, lactate, calcium, phosphorus, copper, cobalt and vitamin E. However, breed may have an effect on potassium and zinc concentration in Arabian and English thoroughbred racehorses (p<0.05.

  5. The usefulness of whole body bone mineral densitometry in the osteopenia of preterm infants: comparison with the wrist radiography and biochemical parameters

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, Bong Jin; Huh, Jin Do; Shin, Sang Bum; Cheon, Byung Kook; Joh, Young Duk [Kosin Univ. Gospel Hospital, Pusan (Korea, Republic of); Kwon, Jeong Mi; Jeon, Seong Sook [Ilsin Christian Hospital, Pusan (Korea, Republic of)

    1997-02-01

    To evaluate the usefulness of whole body bone mineral densitometry in the diagnosis of frequent osteopenia of preterm infants by comparison with the wrist radiographs and biochemical parameters. From January 1995 to January 1996, we obtained whole body bone mineral density(BMD) studies using dual energy X-ray absorptiometry(DXA) and wrist radiographs of 39 preterm infants. They were divided into three groups according to birth weight, under 1500g, 1501g to 2000g and above 2000g, and four grades of skeletal change, as seen on wrist radiography, according to the scoring method of Koo et al. Groups of birth weight and grades of skeletal change were then correlated with whole body BMD and biochemical parameters. For comparison, normal data were obtained from 13 infants born at full term. Data were analyzed by one way analysis of variation(ANOVA) and correlation and regression analysis. A p-value of less than 0.05 was considered significant. Whole body BMDs were significantly lower in the more premature and smaller birth weight infants(r=0.77, p=0.0000), and in the higher grade of skeletal change(r-0.5276, p=0.0000). Aggravated skeletal changes were found in infants with lower birth weight(r= -0.3822, p=0.01). Interobserver variation in grading skeletal change was 42.9%, and intraobserver variation was 18.4%. Biochemical parameters such as serum calcium, phosphate, alkaline phosphatase, parathromone, calcitonin and 25-hydroxy-vitamine D did not vary significantly according to either birth weight or skeletal change(p > 0.05). Premature osteopenia is more effectively diagnosed by measuring whole body BMD using DXA than by grading radiographical skeletal change or by biochemical parameters.

  6. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats.

    Science.gov (United States)

    Beck, W; Gobatto, C

    2016-03-01

    Nocturnal rodents should be assessed at an appropriate time of day, which leads to a challenge in identifying an adequate environmental light which allows animal visualisation without perturbing physiological homeostasis. Thus, we analysed the influence of high wavelength and low intensity light during dark period on physical exercise and biochemical and haematological parameters of nocturnal rats. We submitted 80 animals to an exhaustive exercise at individualised intensity under two different illuminations during dark period. Red light (> 600 nm; experiments.

  7. Effects of medium-chain fatty acids on performance, carcass characteristics, blood biochemical parameters and immune response in Japanese quail.

    Science.gov (United States)

    Saeidi, E; Shokrollahi, B; Karimi, K; Amiri-Andi, M

    2016-06-01

    This study had the aim of evaluating the effects of medium-chain fatty acids (MCFA) on performance, carcass characteristics, some blood parameters and antibody titre against sheep red blood cells (SRBC) in quail. A total of 240 quail chicks were allotted to 4 treatments consisting of respectively 0, 1, 2 and 4 g/kg dietary MCFA. There were no significant differences in body weight, feed intake and feed conversion ratio among treatments at different stages of the experiment. MCFAs had no significant effect on breast, thigh, liver, spleen and bursa of Fabricius weight ratios. However, the relative weight of abdominal fat significantly decreased in quail receiving 0.2 and 0.4 MCFA as compared to other treatments. Concentrations of low-density lipoprotein-cholesterol (LDL), triglycerides and total cholesterol were decreased and high-density lipoprotein (HDL)-cholesterol was increased in quail chicks receiving MCFA compared with control quail chicks. The concentrations of SRBC antibody were not statistically different among treatments. It is concluded that MCFA significantly decreased LDL, triglycerides, cholesterol and abdominal fat and increased HDL in quail chicks.

  8. Protective effect of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaei, S; Nikzad, H; Taghizadeh, M; Tameh, A A; Taherian, A; Moravveji, A

    2014-10-01

    Cancer treatment with cyclophosphamide (CP) may result in reproductive toxicity as one of its side effects. The pumpkin seed is a rich natural source of antioxidant. We have assessed the possible protective efficacy of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology of CP-treated rats. Male adult Wistar rats were categorised into four groups. Group 1 served as control and received intraperitoneal (IP) injection of isotonic saline solution. Group 2 rats were treated with CP by IP injection in a single dose of 100 mg/kg body weight, only once. Group 3 and 4 received CP plus 300 and 600 mg/kg pumpkin seed extract respectively. Six weeks after treatment, sperm characteristics, biochemical parameters and histopathological changes were examined. Results showed that, sperm characteristics in CP-treated rats were significantly decreased. Biochemical analysis results showed that the co-administration of 300 mg pumpkin seed extract could increase the total antioxidant capacity (TAC) level significantly. In CP-treated rats, histopathological changes such as vacuolisation, disorganisation and separation of epididymal epithelium were observed as well. Interestingly, pumpkin seed extract could improve the above-mentioned parameters remarkably in CP-treated rats. Our findings indicated that pumpkin seed extract might be used as protective agent against CP-induced reproductive toxicity.

  9. Biochemical parameters during the urban organic wastes stabilization processes; Estudio de parametros bioquimicos en procesos de estabilizacion de residuos organicos urbanos

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Izquierdo, C.; Polo Sanchez, A.

    1999-07-01

    In this paper, the authors indicate the need for a better understanding of the stabilisation processes of urban organic wastes. The composting process is a method to change fresh organic matter into a material more humificated and less harmful. This process is carried out by a great quantity of microorganisms. We think that the changes in the organic wastes during its stabilisation should be studied by several biological and biochemical parameters (ATP, basal respiration, enzyme activities...). These parameters are good biomarkers of the microbiological activity. (Author) 9 refs.

  10. Effect of non-phytate phosphorus levels and phytase sources on the growth performance, serum biochemical and tibial parameters of broiler chickens

    Directory of Open Access Journals (Sweden)

    Xian R. Jiang

    2013-07-01

    Full Text Available A 3×3 fattorial arrangement with dietary non-phytate phosphorus (NPP levels and phytase sources (3- and 6-phytase was conducted to evaluate the effects of NPP levels, phytase sources and their possible interactions on growth performance, serum biochemical and tibia parameters of broiler chickens from hatch to 42 days of age. A total of 540 1-day-old Arbor Acres male broiler chicks were randomly allocated into nine dietary treatments, each containing 5 replicates pens with 12 birds per pen. Interaction was statistically significant in the performance till day 21 of trial, supplementation of low NPP diet decreased body weight (BW (P<0.001, depressed average daily gain (ADG (P<0.001 and deteriorated average daily feed intake (ADFI (P<0.001 over day 42. During the 8-to-21-day period, even if interaction between NPP levels and phytase sources was significant (P<0.01, BW, ADG and ADFI always increased due to dietary supplementation of phytase, with source not differing. Dietary high NPP enhanced serum calcium and P concentrations on day 21 and 42 (linear contrast, P<0.01, while decreased alkaline phosphatase (AKP activity on day 42 (linear contrast, P<0.001, and interaction was not significant. Both dietary sources of phytase decreased serum AKP activities on day 42 (P<0.001, and urea nitrogen content on day 21 (P<0.01 and 42 (P<0.001. Both phytase improved ash percentage on day 21 and P content in tibia at 21 and 42 days of age (P<0.001. The results confirmed that dietary supplementation of phytase may enhance P availability during the 8-to-21-day period. Nevertheless, no difference between the two phytase sources was observed.

  11. Does Salicylic Acid (SA) Improve Tolerance to Salt Stress in Plants? A Study of SA Effects On Tomato Plant Growth, Water Dynamics, Photosynthesis, and Biochemical Parameters.

    Science.gov (United States)

    Mimouni, Hajer; Wasti, Salma; Manaa, Arafet; Gharbi, Emna; Chalh, Abdellah; Vandoorne, Bertrand; Lutts, Stanley; Ben Ahmed, Hela

    2016-03-01

    Environmental stresses such as salinity directly impact crop growth, and by extension, world food supply and societal prosperity. It is estimated that over 800 million hectares of land throughout the world are salt-affected. In arid and semi-arid regions, salt concentration can be close to that in the seawater. Hence, there are intensive efforts to improve plant tolerance to salinity and other environmental stressors. Salicylic acid (SA) is an important signal molecule for modulating plant responses to stress. In the present study, we examined, on multiple plant growth related endpoints, whether SA applied through the rooting medium could mitigate the adverse effects of salinity on tomato (Solanum lycopersicum) cv. Marmande. The latter is a hitherto understudied tomato plant from the above perspective; it is a classic variety that produces the large ribbed tomatoes in the Mediterranean and consumed worldwide. We found salt stress negatively affected the growth of cv. Marmande tomato plants. However, the SA-treated plants had greater shoot and root dry mass, leaf area compared to untreated plants when exposed to salt stress. Application of SA restores photosynthetic rates and photosynthetic pigment levels under salt (NaCl) exposure. Leaf water, osmotic potential, stomatal conductance transpiration rate, and biochemical parameters were also ameliorated in SA-treated plants under saline stress conditions. Overall, these data illustrate that SA increases cv. Marmande tomato growth by improving photosynthesis, regulation and balance of osmotic potential, induction of compatible osmolyte metabolism, and alleviating membrane damage. We suggest salicylic acid might be considered as a potential growth regulator to improve tomato plant salinity stress resistance, in the current era of global climate change.

  12. Assessment of clinical biochemical parameters in Roma minority residing in eastern Slovakia compared with the majority population.

    Science.gov (United States)

    Hubková, Beáta; Maslanková, Jana; Stupák, Marek; Guzy, Juraj; Kovácová, Anna; Pella, Daniel; Jarcuska, Peter; Mareková, Mária

    2014-03-01

    Roma constitute the largest ethnic minority in Europe and the second largest minority in Slovakia. Their health problems originate mainly from their low socioeconomic status, certain cultural aspects and their health-threatening lifestyle as well as the psycho-social burden arising from poverty and frequent migration. Evaluation of glucose, albumin, triacylglycerol (TAG) and low density lipoprotein cholesterol (LDL-C) concentrations did not reveal any clue about the presumed deteriorated health of the Roma population. Higher proportions of subjects with elevated serum total cholesterol were found in Roma women as compared to both control groups of women (p = 0.027, p = 0.006) and in Roma men as compared to the male control group living in standard conditions. Only the low level of HDL-cholesterol gives a glimpse of their deteriorated health. Significantly lower levels of serum HDL-C were reported in Roma men and women compared to the respondents in both control groups with a p value of p Roma men and men from the control group 1 (p = 0.022) in favour of the control group. When comparing the number of people with physiological values of cholesterols and with worsening TAG parameters at the same time, the increased risk of Roma men compared with men from the control group 1 became evident, with a level of significance of p = 0.023. Evaluation of urine samples pointed to significantly higher concentrations of urinary protein in Roma women compared with women in the control group 1 (p = 0.012).

  13. Effect of atmospheric parameters on fine particulate concentration in suburban Shanghai

    Institute of Scientific and Technical Information of China (English)

    Jian Yao; Wei Liu; Wangkun Chen; Guanghua Wang; Youshi Zeng; Yu Huang; Jun Lin

    2013-01-01

    A study was conducted on the effect of atmospheric parameters,including temperature,wind speed,and relative humidity,on fine particulate mass concentrations measured inJiading District of Shanghai,China,during the period from January 2009 to January 2010.A sensitivity analysis was applied to investigate the interaction between atmospheric parameters and particulate mass concentration.The experiment revealed that the concentration of particulates increased with particle size from 0.1 to 1.0μm,and decreased with the increase of particle size from 1.0 to 2.5 μm.The effects of atmospheric parameters on fine mass concentrations were significantly particle size-dependent.The PM1.0-2.5 may come from the size increase of smaller particulates after moisture absorption.And the variation of concentrations of PM0.1-1.0was mainly attributed to the accumulation of PM0.1.The ventilation index and dilution index were calculated on the basis of data collected in December 2009.A correlation analysis indicated that there was a significant relation between these two indexes and the particulate concentration by examining the three particle size ranges,0.0-0.1,0.1-1.0,and 1.0-2.5 μm.The Spearman correlation coefficients that related the ventilation index to the concentration for the three particle size ranges were-0.45,-0.56 and-0.47,respectively,while the coefficients that related the dilution index to the concentration were-0.36,-0.42and-0.45,respectively.

  14. The relationships between air pollutants, meteorological parameters and concentration of airborne fungal spores

    Energy Technology Data Exchange (ETDEWEB)

    Grinn-Gofron, Agnieszka, E-mail: agofr@univ.szczecin.p [Department of Plant Taxonomy and Phytogeography, Faculty of Natural Science, University of Szczecin, Waska 13 Street, 71-415 Szczecin (Poland); Strzelczak, Agnieszka [Department of Food Process Engineering, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin (Poland); Wolski, Tomasz [Physical Oceanography Laboratory, University of Szczecin (Poland)

    2011-02-15

    Fungal spores are an important component of bioaerosol and also considered to act as indicator of the level of atmospheric bio-pollution. Therefore, better understanding of these phenomena demands a detailed survey of airborne particles. The objective of this study was to examine the dependence of two the most important allergenic taxa of airborne fungi - Alternaria and Cladosporium - on meteorological parameters and air pollutant concentrations during three consecutive years (2006-2008). This study is also an attempt to create artificial neural network (ANN) forecasting models useful in the prediction of aeroallergen abundance. There were statistically significant relationships between spore concentration and environmental parameters as well as pollutants, confirmed by the Spearman's correlation rank analysis and high performance of the ANN models obtained. The concentrations of Cladosporium and Alternaria spores can be predicted with quite good accuracy from meteorological conditions and air pollution recorded three days earlier. - ANN models predict airspore contents from weather conditions and air pollutant.

  15. Impacts of some meteorological parameters on the SO2 concentrations in the City of Obrenovac, Serbia

    Directory of Open Access Journals (Sweden)

    SNEŽANA S. NENADOVIĆ

    2010-05-01

    Full Text Available In this paper, the impacts of some meteorological parameters on the SO2 concentrations in the City of Obrenovac are presented. The City of Obrenovac is located in the north-west part of Serbia on the banks of the River Sava. The observed source emission, the power plants TENT A and B are situated on the bank of the Sava River in the vicinity of Obrenovac. During the period from January to November 2006, the concentrations of sulfur dioxide in the air at 4 monitoring sites in Obrenovac were measured. It was noticed that the maximal measured daily concentrations of sulfur dioxide ranged from 1 μg m-3 (16th November, 2006 to 98 μg m-3 (29th January 2006 and lie under the maximal allowed concentration value according to the Serbian Law on Environmental Protection. The measured sulfur dioxide concentrations mostly showed characteristics usual for a daily acidification sulfur dioxide cycle, excluding the specificities influenced by the measuring site itself. Sulfur dioxide transport was recorded at increased wind speeds, primarily from the southeast direction. Based on the impact of meteorological parameters on the sulfur dioxide concentration, a validation of the monitoring sites was also performed from the aspect of their representivity.

  16. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation.; Efeito da radiacao gama em alguns parametros microbiologicos e bioquimicos da fermentacao alcoolica

    Energy Technology Data Exchange (ETDEWEB)

    Alcarde, Andre Ricardo

    2000-07-01

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  17. Effects of Dietary L-carnitine Supplementation on Growth Performance, Organ Weight, Biochemical Parameters and Ascites Susceptibility in Broilers Reared Under Low-temperature Environment.

    Science.gov (United States)

    Wang, Y W; Ning, D; Peng, Y Z; Guo, Y M

    2013-02-01

    The objective of this study was to investigate the effects of L-carnitine on growth performance, organ weight, biochemical parameters of blood, heart and liver, and ascites susceptibility of broilers at different ages reared under a low-temperature environment. A total of 420 1-d-old male Ross 308 broilers were randomly assigned to two dietary treatments with fifteen replicates of fourteen broilers each. Treatment diets consisted of L-carnitine supplementation at levels of 0 and 100 mg/kg. At 11-d of age, low temperature stress was used to increase ascites susceptibility. Blood, heart and liver samples were collected at different ages for analysis of boichemical parameters. The results showed that, there was no significant difference in growth performance with L-carnitine supplementation, but the mortality due to ascites was significantly decreased. Dietary L-carnitine supplementation significantly reduced heart index (HI) and ascites heart index (AHI) on d 21, lung index (LUI) on d 35 and liver index (LI) on d 42. The broilers fed diets containing L-carnitine had significantly lower red blood cell counts (RBC), hemoglobin (HGB) concentration and hematocrit (HCT) on d 42. Dietary L-carnitine supplementation significantly reduced malondialdehyde (MDA) content of heart tissue on d 21 and 35, and significantly increased total superoxide dismutase (T-SOD) and Glutathione peroxidase (GSH-Px) activity of the heart on d 21 and 42. L-carnitine supplementation significantly reduced serum triglyceride (TG) content on d 28 and 35 and serum glucose (GLU) on d 35 and 42, and significantly increased serum total protein (TP) and globulin (GLO) content on d 42. L-carnitine supplementation significantly enhanced liver succinodehydrogenase (SDH), malic dehydrogenase (MDH) and Na(+)-K(+)-ATPase activity on d 28, and tended to reduce the lactic acid (LD) level of liver on d 35 (p = 0.06). L-carnitine supplementation significantly reduced serum uric acid (UA) content on d 28, 35 and 42

  18. Concentrations of Mineral in Amniotic Fluid and Their Relations to Selected Maternal and Fetal Parameters.

    Science.gov (United States)

    Suliburska, J; Kocyłowski, R; Komorowicz, I; Grzesiak, M; Bogdański, P; Barałkiewicz, D

    2016-07-01

    The concentrations of various trace elements in amniotic fluid (AF) change over the course of pregnancy, with gestational age and fetus growth. The aim of the present study was to evaluate the concentrations of selected essential and toxic elements in AF and their relations to maternal and fetal parameters. The study was carried out in 39 pregnant women, aged 34.6 ± 4.7 years, between weeks 16 and 26 of gestation. Amniotic fluid samples were obtained during the standard procedure of amniocentesis in high-risk patients for chromosomal abnormalities. An inductively coupled plasma mass spectrometry (ICP-MS) technique was used to determine the levels of Al, As, Ba, Cd, Co, Cr, Cu, Mg, Mn, Ni, Sr, U, and V in AF. Body mass and blood pressure were measured in all the women. The basic parameters of fetal development were also assayed. It was found that the age of the mother, the gender of the fetus, and the week of the pregnancy may affect the concentrations of mineral in the amniotic fluid. Moreover, several significant correlations between the essential and toxic elements and maternal and fetal parameters were observed. In particular, negative and positive correlations between fetal parameters and magnesium and copper levels in AF, respectively, were seen. The present findings demonstrate the association between minerals in AF and fetal development.

  19. Immunologic parameters evaluations in Nile tilapia (Oreochromis niloticus) exposed to sublethal concentrations of diazinon.

    Science.gov (United States)

    Girón-Pérez, M I; Velázquez-Fernández, J; Díaz-Resendiz, K; Díaz-Salas, F; Canto-Montero, C; Medina-Díaz, I; Robledo-Marenco, M; Rojas-García, A; Zaitseva, G

    2009-08-01

    Fish resistance to microorganisms depends basically on the immune response. Although there are several studies on the diazinon mammalian immunotoxicity, in the case of fish there are only few. The aim of present study was to evaluate the effect of diazinon on immunological parameters (relative spleen weight, splenocytes count, lysozyme activity, respiratory burst and IgM concentration) in Nile tilapia. Diazinon at sublethal concentrations (0.39 and 0.78 mg/L) did not alter RSW, splenocytes count or lysozyme activity. However, at the highest concentration tested (1.96 mg/L) diazinon significantly increased respiratory burst and IgM concentration. In summary, diazinon (and perhaps other pesticides) could alter immunological response and induce oxidative stress.

  20. [The effects of grape seed and coriander oil on biochemical parameters of oral fluid in patients with periodontitis].

    Science.gov (United States)

    Nikolaishvili, M; Gogua, M; Franchuki, Q; Tufinashvili, T; Zurabashvili, D

    2014-10-01

    Biochemical changes that are taking place in patients with periodontitis 1 and 2 the quality of the individuals place in a laqtatdegidrogenazis, alkaline phosphatase activity increase and reduced activity amilazais. Therefore we can conclude that, there is an increase in superoxide substances, which causes an increase in the oral cavity patobakteriebis and quality of periodontitis. The grape seed and coriander oil is of vegetable origin and antioxidant drugs. Their action causes a statistically significant increase in the amilazis, alkaline phosphatase and laqtatdegidrogenazis reduction, while the latter leads pH - rate of return to oral fluid. It should be noted that the positive effect of coriander oil, but less effective.

  1. Optimal Dearsenification Parameters of Gold Sulfide Concentrate with a High As Content

    Institute of Scientific and Technical Information of China (English)

    Yaozhong LAN; Rose W. Smith

    2005-01-01

    The gold sulfide concentrate with a high As content in Liangshan District, Sichuan Province, China, is a potentially important resource. This paper describes experiments of dearsenification of gold concentrate in a weakly reduced atmosphere in a rotary pipe furnace. The results showed that the optimal parameters were a temperature range of 650~700℃, 15%~16% CO2 of gas and a reaction time of 30~40 min. The removal rate of arsenic and sulfur was over 95% and 25%~28%, respectively. With further oxidization and roasting, residue sulfur in the roasted materials was dropped to below 4%, and the cyanide leaching recovery of gold was over 92%.

  2. Biochemical liver function test parameter levels in relation to treatment response in liver metastatic colorectal patients treated with FOLFOX4 with or without bevacizumab

    Directory of Open Access Journals (Sweden)

    Denić Kristina

    2016-01-01

    Full Text Available Introduction. Combined use of bevacizumab and conventional anticancer drugs leads to a significant improvement of treatment response in patients with metastatic colorectal carcinoma (CRC. Conventional treatment protocols exert undesired effects on the liver tissue. Hepatotoxic effects are manifested as a disturbance of liver function test parameters. The relation between clinical outcome and disorder of biochemical parameters has not been completely evaluated. Objective. The objective of our study was to examine whether clinical outcome in patients with liver metastatic CRC correlates with the level of liver function test parameters. Methods. The study included 96 patients with untreated liver metastatic CRC who received FOLFOX4 protocol with or without bevacizumab. Biochemical liver parameters were performed before and after the treatment completion. Treatment response was evaluated as disease regression, stable disease, and disease progression. The patients were divided into three groups according to the accomplished treatment response. Results. In the group of patients with disease regression the post-treatment levels of aspartate aminotransferase, alanine aminotransferase, and bilirubin were statistically significantly increased. In contrast to this, gamma-glutamyltransferase and protein post-treatment values were significantly lower in relation to initial values. In patients with stable disease, difference was found only in the level of proteins being lower after the treatment. In patients with disease progression, values of aspartate aminotransferase and bilirubin were significantly increased after completed treatment. Conclusion. Treatment responses are not completely associated with the level of liver function test parameters. The only parameter which correlated with treatment response is gamma-glutamyltransferase. Its decrease is accompanied with disease regression.

  3. Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaie, Somaieh; Nikzad, Hossein; Mahabadi, Javad Amini; Taghizadeh, Mohsen; Azami-Tameh, Abolfazl; Taherian, Aliakbar; Sajjadian, Seyyed Mohammad Sajjad; Kamani, Mehran

    2016-09-01

    Reproductive toxicity is one of the side effects of cyclophosphamide (CP) in cancer treatment. Pumpkin seeds and Zingiber officinale are natural sources of antioxidants. We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats. Male adult Wistar rats were divided randomly into six groups. Group 1, as a control, received an isotonic saline solution injection intraperitoneally (IP). Group 2 were injected IP with a single dose of CP (100 mg/kg) once. Groups 3 and 4 received CP plus 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract (50:50). Groups 5 and 6 received only 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract. Six weeks after treatment, sperm characteristics, histopathological changes and biochemical parameters were assessed. In CP-treated rats, motile spermatozoa were decreased, and abnormal or dead spermatozoa increased significantly (P < 0.001) but administration of the mixed extract improved sperm parameters. Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. Biochemical analysis showed that the administration of combined extracts could increase the total antioxidant capacity (TAC) level significantly in groups 3, 4, 5 and 6. Interestingly, the mixed extract could decrease most of the side effects of CP such as vacuolization and separation of epididymal tissue. Our findings indicated that the combined extracts might be used as a protective agent against CP-induced reproductive toxicity.

  4. Hemato - Immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri.

    Science.gov (United States)

    Adel, Milad; Pourgholam, Reza; Zorriehzahra, Jalil; Ghiasi, Maryam

    2016-08-01

    This study was aimed to assess the potential effects of Mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against Yersinia ruckeri infection in rainbow trout Oncorhynchus mykiss. Fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of Mentha piperita (MP) plant extract for 8 weeks. Dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red and white cells, hematocrit and hemoglobin contents), as well as in respiratory burst activity, total protein, albumin, and neutrophil levels in fish fed supplemented diets compared to the control fish. Furthermore, dietary MP plant extract supplements have no significant effect on blood biochemical parameters and enzymatic activities of liver determined in serum of rainbow trout. After 8 weeks the cessation of feeding with MP plant extract, survival rates of 54.4%, 63.6% and 75.2% were recorded in groups which received 1, 2 and 3% of MP plant extract of feed, respectively, compared to 34.6% survivals in the control. This study underlying several positive effects of dietary administration of MP plant extract to farmed fish.

  5. Prognostic value of metabolic parameters and clinical impact of {sup 18}F-fluorocholine PET/CT in biochemical recurrent prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Colombie, M.; Bailly, C.; Rusu, D.; Rousseau, N. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Campion, L. [ICO Cancer Center, Statistics, Saint-Herblain (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, T. [Urologic Clinic Nantes-Atlantis, Saint Herblain (France); Mathieu, C. [University Hospital, Nuclear Medicine, Nantes (France); Ferrer, L. [ICO Cancer Center, Physics, Saint-Herblain (France); Kraeber-Bodere, F. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); University Hospital, Nuclear Medicine, Nantes (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, C. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France)

    2015-11-15

    To evaluate the therapeutic impact of {sup 18}F-fluorocholine (FCH) PET/CT in biochemical recurrent prostate cancer (PC) and to investigate the value of quantitative FCH PET/CT parameters in predicting progression-free survival (PFS). This retrospective study included 172 consecutive patients with PC who underwent FCH PET/CT for biochemical recurrence. Mean rising PSA was 10.7 ± 35.0 ng/ml. Patients with positive FCH PET were classified into three groups: those with uptake only in the prostatic bed, those with locoregional disease, and those with distant metastases. Referring physicians were asked to indicate the hypothetical therapeutic strategy with and without the FCH PET/CT results. Clinical variables and PET parameters including SUVmax, SUVpeak, SUVmean, total lesion choline kinase activity (TLCKA) and standardized added metabolic activity (SAM) were recorded and a multivariate analysis was performed to determine the factors independently predicting PFS. In 137 of the 172 patients, the FCH PET/CT scan was positive, and of these, 29.9 % (41/137) had prostatic recurrence, 42.3 % (58/137) had pelvic lymph node recurrence with or without prostatic recurrence, and 27.7 % (38/137) had distant metastases. The FCH PET/CT result led to a change in treatment plan in 43.6 % (75/172) of the 172 patients. Treatment was changed in 49.6 % (68/137) of those with a positive FCH PET/CT scan and in 20 % (7/35) of those with a negative FCH PET/CT scan. After a median follow-up of 29.3 months (95 % CI 18.9 - 45.9 months), according to multivariate analysis age <70 years, SAM ≥23 and SUVmean ≥3 were parameters independently predicting PFS. A nomogram constructed using the three parameters showed 49 months of PFS in patients with the best scores (0 or 1) and only 11 months in patients with a poor score (score 3). This study indicates that a positive FCH PET result in PC patients with biochemical recurrence predicts a shorter PFS and confirms the major impact of the FCH PET

  6. The Redshift Evolution of LCDM Halo Parameters: Concentration, Spin, and Shape

    CERN Document Server

    Muñoz-Cuartas, J C; Gottlöber, S; Dutton, A A

    2010-01-01

    We present a detailed study of the redshift evolution of dark matter halo structural parameters in a LambdaCDM cosmology. We study the mass and redshift dependence of the concentration, shape and spin parameter in Nbody simulations spanning masses from 10^{10} Msun/h to 10^{15} Msun/h and redshifts from 0 to 2. We present a series of fitting formulas that accurately describe the time evolution of the concentration-mass relation since z=2. Using arguments based on the spherical collapse model we study the behaviour of the scale length of the density profile during the assembly history of haloes, obtaining physical insights on the origin of the observed time evolution of the concentration mass relation. We also investigate the evolution with redshift of dark matter halo shape and its dependence on mass. Within the studied redshift range the relation between halo shape and mass can be well fitted by a power law. Finally we show that although for z=0 the spin parameter is practically mass independent, at increasi...

  7. The Polyphenols Stability, Enzyme Activity and Physico-Chemical Parameters During Producing Wild Elderberry Concentrated Juice

    Directory of Open Access Journals (Sweden)

    Ante Galić

    2009-12-01

    Full Text Available The influence of processing wild elderberry into concentrated juice on polyphenols (total phenols, flavonoids, non-flavonoids, anthocyanins, flavan-3-ols, hydrolysed tannins stability, activity of polyphenol oxidase (PPO and peroxidase (POD, and changes of physico-chemical parameters (total and soluble dry matter, total acidity, pH, sugars were investigated. The amounts of total phenols, flavonoids, non-flavonoids, falvan-3-ols and hydrolysed tannins were analyzed using Folin-Ciocalteu colorimetric method, while the total anthocyanins were determined by bisulphite bleaching method. Total phenols ranged from 25.87 mg/g DM to 38.87 mg/g DM. Total anthocyanins were the most abundant polyphenols in all investigated samples (raw elderberries, elderberries after blanching, elderberry juice after disintegration and pressing, concentrated elderberry juice and their concentration ranged from 13.12 mg/g DM to 25.67 mg/g DM. Other polyphenols determined in high concentration were hydrolysed tannins, followed by fl avan-3-ols, flavonoids and nonfavonoids. After blanching, the concentration of all polyphenols did not decrease significantly. After disintegration of elderberries the concentration of all polyphenols increased, probably due to inactivation of PPO and POD and better isolation of polyphenols from homogenized puree. During processing of elderberry juice into concentrated juice most polyphenols were stable. Total acidity and pH value were not changed during processing, whereas the amounts of total and reducing sugar increased after pressing and additionally after concentration. The obtained results suggest that raw elderberries as well as elderberry concentrated juice are high potential source of polyphenols especially anthocyanins.

  8. Effect of Subacute Exposure of Wrightia tinctoria Bark Extract on Hematological, Biochemical and Antioxidant Enzyme Parameters of Rat

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    Papiya Bigoniya

    2009-01-01

    The present study was undertaken to investigate the effect of sub-acute administration of W. tinctoria bark extract on some haematological, biochemical, histological and antioxidant enzyme status of rat liver and kidney following 21 and 45 days treatment. The animals were observed for gross physiological and behavioural responses, food and water intake and body weight changes. Free radical scavenging activity and histopathology was done on liver and kidney samples. W. tinctoria showed significant hemopoiesis with increase in body weight signifying anabolic effect. It significantly reduced serum SGOT level and increased glucose levels. W. tinctoria caused increased SOD activity of liver along with catalase of both liver and kidney and decreased liver peroxidase (P< 0.001. These features indicate that W. tinctoria upto 1000 mg/kg daily dose is safe and has potential to be consumed for long time in management of various diseases.

  9. Correlation of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function in Chinese patients with chronic hepatitis C virus infection

    Institute of Scientific and Technical Information of China (English)

    Zheng-Wen Liu; Ni Zhang; Qun-Ying Han; Jun-Tao Zeng; Yong-Lie Chu; Jian-Ming Qiu; Ya-Wen Wang; Lie-Ting Ma; Xiao-Qin Wang

    2005-01-01

    AIM: To determine serum leptin levels and investigate their correlations with anthropometric and metabolic parameters and biochemical liver function in patients with chronic hepatitis C virus (HCV) infection and their potential clinical implications.METHODS: Forty-two chronic HCV-infected patients without anti-viral treatment were enrolled in this study, 30 patients had chronic hepatitis C, 10 had cirrhosis, and 2 had hepatocellular carcinoma (HCC). Thirty age- and sex-matched healthy individuals served as controls. Serum leptin levels were determined by ELISA. The biochemical liver function and serum lipids were determined at the same time. The height and body weight of patients and controls were measured, and body mass index (BMI) and body fat were calculated simultaneously. The correlations of serum leptin levels with anthropometric and metabolic parameters and biochemical liver function were assessed statistically.RESULTS: The mean of serum leptin levels in patients with chronic hepatitis C, HCV-associated cirrhosis, HCVassociated HCC and control groups was (6.13±3.94),(5.25±4.21), (4.17±0.28), and (3.59±3.44) ng/mL, respectively. The serum leptin level in patients with chronic hepatitis C was significantly higher than that in controls. The serum leptin levels between cirrhotic patients and controls and between male and female cirrhotic patients had no significant difference. Serum leptin levels were positively-correlated with body fat, BMI, and apolipoprotein B (Apo B) in patients with chronic HCV infection. The serum alanine aminotransferase (ALT) levels were closelycorrelated with BMI in patients with chronic hepatitis C. CONCLUSION: HCV infection interferes with fat and lipid metabolism in patients with chronic HCV infection and leptin may play a role in hepatosteatosis.

  10. Effects of magnetic treated water on serum concentration parameters and fat thickness

    Directory of Open Access Journals (Sweden)

    Geraldo Balieiro Neto

    2013-02-01

    Full Text Available The goal of this study was to evaluate the effect of magnetic water on blood cells counts, biochemical profile, blood gas level and subcutaneous fat thickness of Jersey cows. This research was carried out at Agência Paulista de Tecnologia dos Agronegócios - APTA, SP. Twenty six Jersey cows from the APTA were allotted into two groups: control (n=13, drinking regular water and the group consuming magnetic water (n=13. The animals were lactating around 150 days and pregnant around 60 days. Blood samples were collected from caudal auricular artery and jugular vein. The water treatment had no effect on hemogram (p>0.05. Higher pH (7.448 vs 7.407 mmHg, p<0.05 and lower PaCO2 (37.97 vs 42.47 mmHg, p<0.05 levels were detected in arterial blood of the group drinking magnetic water. The concentration of Na ion (138.8 vs 145.5 mmol/l, p<0.05 and serum triglycerides (10.4 vs 22.6 mg/dL, p<0.05 were significantly lower, resulting in smaller osmolality (273.30 vs 280.99 mOsm/kg, p<0.05 and subcutaneous fat thickness (0.2 vs 1.3 mm, p<0.05. In summary, the water treatment, evaluated in this study, may have decreased the risk of some metabolic disorders, such as acidosis and high serum concentration of Na and carbon dioxide.

  11. Assessment of serum biochemical parameters and pathological changes in broilers with chronic aflatoxicosis fed glucomannan-containing yeast product (Mycosorb and sodium bentonite

    Directory of Open Access Journals (Sweden)

    Azizpour Aidin

    2015-06-01

    Full Text Available The purpose of this study was to evaluate the efficacy of yeast glucomannan (YG and sodium bentonite (SB in reducing the toxicity in broilers fed a diet naturally contaminated with aflatoxin. Three hundred 7-day-old Ross 308 strain broilers were chosen and randomly assigned to 10 dietary treatments. Serum biochemical parameters and pathological changes in the liver were investigated at 42 d of age. Chickens fed a diet containing 250 ppb of aflatoxin displayed a decrease in uric acid, cholesterol, and triglycerides, and an increase in serum activities of AST and ALT when compared to control group. There were considerable gross and histopathological hepatic lesions (P < 0.05 in the form of small to moderate hydropic and/or fatty degeneration, bile duct hyperplasia, periportal fibrosis, cells infiltration, and congestion, in chickens fed the 250 ppb aflatoxincontaining diet. The addition of YG and SB to the aflatoxin-containing diet partially reduced the negative effects of aflatoxin. The 0.1% YG supplementation to the aflatoxin-contaminated diet significantly prevented the pathological effect of aflatoxin on serum biochemical parameters and liver, and was found to be more effective than other treatments.

  12. Analysis of oxidative status and biochemical parameters in adult patients with sickle cell anemia treated with hydroxyurea, Ceará, Brazil

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    Paulo Florentino Teixeira Neto

    2011-06-01

    Full Text Available BACKGROUND: Sickle cell anemia is a hemoglobinopathy caused by a mutation that results in the production of an abnormal hemoglobin molecule, hemoglobin S (Hb S. This is responsible for profound physiological changes, such as the sickling of red blood cells. Several studies have shown that hydroxyurea protects against vaso-occlusive crises. OBJECTIVE: The aim of this study was to evaluate the oxidative stress associated with biochemical parameters in patients with sickle cell anemia treated with hydroxyurea. METHODS: The study was conducted with 20 male and 25 female patients at the Hospital Universitário Walter Cantídio. The patients were divided into two groups: a study group (n = 12, patients with sickle cell anemia who were receiving hydroxyurea and a control group (n = 33 of sickle cell anemia patients not submitted to hydroxyurea treatment. The biochemical parameters analyzed were ferritin, transferrin, and serum iron. Glutathione was measured in its reduced form to analyze the oxidative state. RESULTS: The results showed insignificant increases in the levels of serum iron, transferrin and ferritin in patients treated with hydroxyurea when compared with those who did not take the medication. However, the glutathione levels were significantly higher in patients taking hydroxyurea than in controls. CONCLUSION: These results indicate that hydroxyurea possibly acts as an antioxidant by increasing glutathione levels.

  13. Effect of tocopherol on biochemical blood parameters in pleuritis-induced rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Science.gov (United States)

    Całkosiński, Ireneusz; Rosińczuk-Tonderys, Joanna; Bronowicka-Szydełko, Agnieszka; Dzierzba, Katarzyna; Bazan, Justyna; Dobrzyński, Maciej; Majda, Jacek; Gamian, Andrzej

    2015-06-01

    The aim of this study was to evaluate the effect of tocopherol on pleuritis-induced rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single TCDD dose of 5 μg/kg body weight (b.w.) and then for 3 weeks they were daily supplemented with tocopherol at a dose of 30 mg/kg b.w. The inflammation was initiated by intrapleural injection of a single dose of 1% carrageenin solution in a volume of 0.15 ml. Changes in biochemical blood parameters were measured three times at the 24th, 72nd and 120th hour of pleuritis and the blood was collected from 20 animals of each group of rats (group with the control inflammation; group treated with TCDD and with control inflammation; group treated with TCDD, supplemented with tocopherol and with the inflammation). The following biochemical parameters were measured: tumor necrosis factor (TNF), interleukin-1 (IL-1), IL-2, IL-4, IL-6, procollagen, telopeptide, fibrinogen, cholesterol, urea, creatinine, aspartate aminotransferase (AspAT) and alanine aminotransferase (AlAT). Daily supplementation of tocopherol caused significant changes in the level of TNF, IL-1, IL-4, IL-6, urea, creatinine, AspAT and AlAT. According to the results of these studies, we suggest that tocopherol supplementation in high doses could act as a protective treatment to improve liver metabolism.

  14. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia.

  15. The phytoconstituents and the comparative effects of aqueous extract of Irvingia gabonensis seeds and proviron on the biochemical parameters of male guinea pigs

    Institute of Scientific and Technical Information of China (English)

    Obianime AW; Uche FI

    2010-01-01

    Objective:To investigate the phytochemical screening and the effects of the aqueous extracts of the seeds ofIrvingia gabonensis on the biochemical parameters of male guinea pigs.Methods: The biochemical parameters were assayed using Randox Diagnostic kits, Phenolphthalein method and colorimetric method. The phytochemical screening was carried out using standard procedures.Results: Phytochemical investigations revealed the presence of flavonoids, tannins, carbohydrate, alkaloids, terpenoids, steroids, volatile oils, saponins and cardiac glycosides. The aqueous extract ofIrvingia gabonensis seeds (50 - 400 mg/kg) caused a statistically significant (P< 0.05 ANOVA) decrease in the levels of total cholesterol, urea, uric acid, total protein, prostatic, alkaline, and acid phosphatases. The highest reduction effect was obtained with uric acid at 400 mg/kg ofIrvingia gabonensis extract while the least effect was observed in total cholesterol. These effects were dose- and time- dependent.Conclusions: This shows that the seeds of Irvingia gabonensis have hepatoprotective, nephroprotective and cardio protective properties. The study therefore, supports the claims on the use of the seeds of this plant by traditional medicine practitioners as a hepatoprotective and nephroprotective agent. Although further studies need to be done to isolate, identify and characterize the active principles in the seeds of this plant.

  16. Does the adequacy parameter Kt/V(urea reflect uremic toxin concentrations in hemodialysis patients?

    Directory of Open Access Journals (Sweden)

    Sunny Eloot

    Full Text Available Hemodialysis aims at removing uremic toxins thus decreasing their concentrations. The present study investigated whether Kt/V(urea, used as marker of dialysis adequacy, is correlated with these concentrations. Predialysis blood samples were taken before a midweek session in 71 chronic HD patients. Samples were analyzed by colorimetry, HPLC, or ELISA for a broad range of uremic solutes. Solute concentrations were divided into four groups according to quartiles of Kt/V(urea, and also of different other parameters with potential impact, such as age, body weight (BW, Protein equivalent of Nitrogen Appearance (PNA, Residual Renal Function (RRF, and dialysis vintage. Dichotomic concentration comparisons were performed for gender and Diabetes Mellitus (DM. Analysis of Variance in quartiles of Kt/V(urea did not show significant differences for any of the solute concentrations. For PNA, however, concentrations showed significant differences for urea (P<0.001, uric acid (UA, p-cresylsulfate (PCS, and free PCS (all P<0.01, and for creatinine (Crea and hippuric acid (HA (both P<0.05. For RRF, concentrations varied for β₂-microglobulin (P<0.001, HA, free HA, free indoxyl sulfate, and free indole acetic acid (all P<0.01, and for p-cresylglucuronide (PCG, 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (CMPF, free PCS, and free PCG (all P<0.05. Gender and body weight only showed differences for Crea and UA, while age, vintage, and diabetes mellitus only showed differences for one solute concentration (UA, UA, and free PCS, respectively. Multifactor analyses indicated a predominant association of concentration with protein intake and residual renal function. In conclusion, predialysis concentrations of uremic toxins seem to be dependent on protein equivalent of nitrogen appearance and residual renal function, and not on dialysis adequacy as assessed by Kt/V(urea. Efforts to control intestinal load of uremic toxin precursors by dietary or other

  17. Effect of Mobile Phone Radiations on Morphological and Biochemical Parameters of Mung Bean (Vigna radiata and Wheat (Triticum aestivum Seedlings

    Directory of Open Access Journals (Sweden)

    Mobin Afzal

    2012-03-01

    Full Text Available Over the last two decades an enormous escalation in wireless equipments has been made our lives easy. The uses of wireless accessories including mobile phone have been enabled instant our communication resources advance and fast, on the other hand along with their advantages there is great increase in radiations, an unprecedented type of pollution, in our environment. Current study was an attempt to observe the morphological and biochemical changes induced by cell phone radiations on Mung bean (Vigna radiata and Wheat (Triticum aestivum seedlings. The effect of cell phone Electromagnetic Radiations (EMR were determined by measuring membrane damage in terms of melondialdehyde [MDA] contents and changes in the levels of antioxidant enzymes like Guaiacol Peroxidase (GPX, Ascorbate Peroxidase (APX, and Catalase (CAT. Our results showed that cell phone EMR caused significant reduction in growth, fresh weight, dry weight, and relative water contents. Where as MDA contents and antioxidant enzymes were increased in stressed seedlings as compared to unstressed seedlings. We concluded that radiations emitted by mobile phone can induce oxidative stress which results in reduced growth and increase in the activity of antioxidant enzymes in mung bean and wheat seedlings.

  18. Influence of meteorological parameters on Olea pollen concentrations in Córdoba (south-western Spain).

    Science.gov (United States)

    Vázquez, L M; Galán, C; Domínguez-Vilches, E

    2003-12-01

    The influence of meteorological parameters on the dispersion of airborne pollen has been studied by several authors. Olive pollen is the major cause of allergy in southern Spain, where a large part of the arable surface area is given over to olive cultivation. Daily pollen forecasts provide important information both for pollen-allergy sufferers and for agronomists trying to achieve a better biological understanding of variations in airborne olive pollen levels. The main purpose of this paper is to study, by means of short-term statistical analysis, the effect of meteorological parameters on airborne olive pollen concentrations in the city of Cordoba (south-western Spain). Twenty-one-year (1982-2002) aerobiological and meteorological databases were used. Correlation and multiple regression analyses were used to study the relationships between olive pollen levels and several meteorological parameters. Statistical analysis was applied both to the whole pollen season and to the pre-peak period. Daily meteorological parameters, such as accumulated mean temperature, accumulated sunlight hours, and accumulated rainfall were used as independent variables in both statistical analyses. Accumulated meteorological variables were of the greatest value in most regression analysis equations, heat-related variables being the most important.

  19. Optimizing Design Parameters for Sets of Concentric Tube Robots using Sampling-based Motion Planning

    OpenAIRE

    Baykal, Cenk; Torres, Luis G.; Alterovitz, Ron

    2015-01-01

    Concentric tube robots are tentacle-like medical robots that can bend around anatomical obstacles to access hard-to-reach clinical targets. The component tubes of these robots can be swapped prior to performing a task in order to customize the robot’s behavior and reachable workspace. Optimizing a robot’s design by appropriately selecting tube parameters can improve the robot’s effectiveness on a procedure-and patient-specific basis. In this paper, we present an algorithm that generates sets ...

  20. The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs

    Directory of Open Access Journals (Sweden)

    Krsnik Mladen

    2010-03-01

    Full Text Available Abstract Background The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance. Methods Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding. Results No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test observed in individual groups before and after the feeding trial are related to the growth of pigs. Conclusion Diet with acids and tannin did not improve the

  1. Parameter optimization of solar modules based on lens concentrators of radiation and cascade photovoltaic converters

    Science.gov (United States)

    Andreev, V. M.; Davidyuk, N. Yu.; Ionova, E. A.; Pokrovskii, P. V.; Rumyantsev, V. D.; Sadchikov, N. A.

    2010-02-01

    Two main issues governing the design of a solar concentrator module with triple-junction nano-heterostructure photovoltaic converters (PVCs) are considered: the effective concentration of radiation using Fresnel lenses and effective heat removal from PVCs. By theoretically and experimentally simulating these processes, the design parameters of module’ s elements are determined. A test batch of full-size modules has been fabricated. Each module consists of a front panel of small-size Fresnel lenses (a total of 144 lenses arranged as a 12 × 12 array) and the corresponding number of multilayer InGaP/GaAs/Ge PVCs. The PVCs are mounted on heat-distributing plates and are also integrated into a panel. The efficiency of the concentrator module with a 0.5 × 0.5-m entrance aperture measured under outdoor conditions is 24.3%, which is more than twice as high as the efficiency of standard (concentrator-free) silicon modules. In smaller test modules, the efficiency corrected for the PVC standard temperature (25° C) reaches 26.5%.

  2. Single sublethal dose of microcystin-LR is responsible for different alterations in biochemical, histological and physiological renal parameters.

    Science.gov (United States)

    Lowe, J; Souza-Menezes, J; Freire, D S; Mattos, L J; Castiglione, R C; Barbosa, C M L; Santiago, L; Ferrão, F M; Cardoso, L H D; da Silva, R T; Vieira-Beiral, H J; Vieyra, A; Morales, M M; Azevedo, S M F O; Soares, R M

    2012-05-01

    Microcystins (MCYSTs) are very stable cyclic peptidic toxins produced by cyanobacteria. Their effects on hepatic tissue have been studied extensively, and they are considered to be a potent hepatotoxin. However, several effects of MCYST on other organs have also been described, but generally in studies using higher doses of MCYST. In the present work, we investigated the effect of a single sublethal dose of MCYST-LR (55 μg/kg) in Wistar rats and analyzed different aspects that influenced renal physiology, including toxin accumulation, excretion, histological morphology, biochemical responses and oxidative damage in the kidney. After 24 h of exposure to MCYST-LR, it was possible to observe an increased glomerular filtration rate (6.28 ± 1.56 vs 2.16 ± 0.48 μl/min per cm(2)) compared with the control group. Increase of interstitial space and collagen deposition corresponded to a fibrotic response to the increased production of reactive oxygen species. The observed decrease of Na(+) reabsorption was due to inhibition of the activity of both Na(+) pumps in proximal tubules cells. We suggested that this modulation is mediated by the effect of MCYST as a phosphatase protein inhibitor that maintains the sustained kinase-mediated regulatory phosphorylation of the ATPases. The observed alteration of Na(+) active transporters lead to damage of renal function, since are involved in regulation of water and solute reabsorption in proximal tubules. The results of this report reinforce the importance of understanding the molecular effects of a single sublethal dose of MCYST-LR, which, in this study, was responsible for macro-alterations found in the renal parenchyma and renal physiology in rats.

  3. The clinical spectrum of renal osteodystrophy in 57 chronic hemodialysis patients: a correlation between biochemical parameters and bone pathology findings.

    Science.gov (United States)

    Chazan, J A; Libbey, N P; London, M R; Pono, L; Abuelo, J G

    1991-02-01

    Fifty-nine chronic hemodialysis patients who had been on dialysis for an average of 77 months underwent bone biopsies and the pathologic findings were correlated with biochemical and demographic data. All but two had evidence of renal osteodystrophy, 23 with osteitis fibrosa (OF), 19 with osteomalacia and/or adynamic disease (OM/AD), and 15 with mixed osteodystrophy (MOD). Patients in each group were similar with regard to age, sex distribution, duration of dialysis, unstimulated serum aluminum, calcium and phosphorus. Patients with osteitis fibrosa (OF) had statistically higher DFO stimulated aluminum, alkaline phosphatase and PTHC levels than the other two groups although there was marked individual variation. The bone biopsies were also evaluated for the amount of aluminum deposited in the osteoid seam. All 23 of the patients with OF and 11 of the 15 patients with MOD had no, mild, or minimal aluminum deposition but 12 of the 19 patients with OM/AD had moderate to marked aluminum deposition. Patients with minimal to mild aluminum deposition were similar in age, duration of dialysis, sex distribution, unstimulated and DFO stimulated aluminum levels, calcium, phosphorus, alkaline phosphatase to those with moderate to marked deposition but had significantly higher parathormone levels. All patients had been treated in a similar fashion regarding diet, oral phosphate binders and vitamin D; therefore, the observed differences in bone pathology were not readily explicable. However, patients who were found to have osteitis fibrosa and those with minimal to mild aluminum deposition had significantly higher parathormone levels when compared with patients in the other groups at the inception of dialysis.

  4. STUDY OF CORRELATION AND SIGNIFICANCE OF BIOCHEMICAL PARAMETERS IN THE ASSESSMENT OF ACTIVE PHASE OF THYROID EYE DISEASE

    Directory of Open Access Journals (Sweden)

    Ashok Kumar P

    2016-08-01

    Full Text Available AIM Role of Interleukin-6 and HS-CRP Levels in the assessment of active thyroid eye disease. MATERIALS & METHODS A prospective observational study of 30 patients from the age group of 20-60 years with thyroid eye disease done at Regional Institute of Ophthalmology. All patients were subjected to detailed history, ocular examination, systemic examination, biochemical analysis and Imaging. Patients are categorised as mild, moderate and severe active disease based on clinical activity score. For the period of two years, patients are followed at regular periodic intervals according to the severity of disease and results were analysed. RESULTS Out of 30 patients studied, majority of the patients were females (60%, and 70% of the patients had bilateral disease. Among 30 patients, 83.33% in hyperthyroid state, 3.33% in Hypothyroid state, 13.33% in Euthyroid state (Table 3. Smoking being important risk factor in 30% among males. 50% of patients presented with mild disease, 30% with moderate disease, 0% with severe disease. IL-6, HS-CRP levels are increased only in patients with severe active disease (Table 6. Remission attained in all patients when treated earlier with steroids. CONCLUSION Identifying disease activity early and aggressive treatment with systemic steroids in active phase of moderate and severe disease has reduced the morbidity associated with disease. Correlation of IL-6, HS-CRP, TFT levels are significantly increased only in patients with active phase of severe thyroid eye disease but not significantly elevated in active phase of moderate disease. Another pitfall is IL-6 is an expensive ELISA based diagnosis. Thus, IL-6 & HS-CRP cannot be routinely used to screen patients with Thyroid eye disease.

  5. Associations between annual and seasonal variations in body mass and reproductive success and blood biochemical parameters in semi-domesticated reindeer

    Directory of Open Access Journals (Sweden)

    Ingrid H. Holmøy

    2009-12-01

    Full Text Available The main objective of the study was to follow reproductive performance and blood biochemical parameters associated with fat and carbohydrate metabolism in a herd of free-ranging, semi-domesticated reindeer (Rangifer tarandus tarandus over a two-year period, with extreme between-year variation in forage availability. The effects of climatic factors on reindeer reproductive performance were investigated by analysing whether time of onset of luteal function in autumn and calf survival were associated with changes in body mass and weather conditions, such as snow depth, precipitation, and temperature. Considerable between-year variation in the onset of luteal activity was found. In 1997, 4.2% of the female reindeer were either cycling or pregnant in the second week of October, whilst in 1998, in the same week, 100% were cycling or pregnant. Although energy balance was important for timing of the onset of luteal activity, delayed conception had no apparent effect on calf survival. The results indicated that maternal body mass (BM in spring was of primary importance for calf survival, and the productivity of the herd. Since climatic factors influence the availability of forage, and hence female BM, it also has an indirect impact on calf survival. Females with low BM demonstrated greater seasonal variation in BM than heavier females. Plasma concentrations of free fatty acids and β-hydroxy butyric acid responded to changes in forage availability, but the initial condition of the reindeer and their fat reserves also seemed to have a major influence on these parameters.Abstract in Norwegian / Sammendrag:Sammenheng mellom års- og sesongvariasjon i kroppsvekt og henholdsvis reproduksjonssuksess og biokjemiske blodparametre hos tamreinHovedformålet med studien var å følge reproduksjonen og blodparametre knyttet til fett og karbohydratmetabolismen hos en tamreinflokk (Rangifer tarandus tarandus gjennom en toårs periode med stor årlig variasjon i n

  6. Determination of acute toxicity and the effects of sub-acute concentrations of CuO nanoparticles on blood parameters in Rutilus rutilus

    Directory of Open Access Journals (Sweden)

    Abdolreza Jahanbakhshi

    2015-07-01

    Full Text Available Objective(s:Copper oxidenanoparticles have different industrial applications so it is inevitable that nanoparticulate products finally find their way into aquatic ecosystems. Nevertheless there is little information available about their effects on some of edible fish. The present study aims to determine the acute toxicity and evaluate the effect of two sub-acute concentrations (50 and 70% 96 h LC50 of CuO-NPs on some hematological and biochemical parameters of R. rutilus. Materials and Methods:225 healthy specimen of R. rutilus (mean weight 5.52±1.2 g; mean length 6.20±0.2 cm were transported to the laboratory. In order to prepare the stock solution, CuO-NPs was dispersed in pure water with ultrasonication (50-60 kHz for 15 min every day before dosing. At first, R. rutilus was exposed to CuO-NPs to determine the lethal concentration (LC50 value. Following acute test, fish were treated with sub-acute concentrations of CuO-NPs (50 and 70% 96 h-LC50 at with one control group (no CuO-NPs for a week to determine the changes in the level of some plasma hematological and biochemical parameters. Results:The 96 h-LC50 values of CuO-NPs was 2.19±0.003 mg/l. R. rutilus exhibited significantly lower RBC count, Hb and Hct values and a significant increase in the WBC numbers, MCH, MCHC and MCV indices (p

  7. SITE SPECIFIC REFERENCE PERSON PARAMETERS AND DERIVED CONCENTRATION STANDARDS FOR THE SAVANNAH RIVER SITE

    Energy Technology Data Exchange (ETDEWEB)

    Jannik, T.

    2013-03-14

    The purpose of this report is twofold. The first is to develop a set of behavioral parameters for a reference person specific for the Savannah River Site (SRS) such that the parameters can be used to determine dose to members of the public in compliance with Department of Energy (DOE) Order 458.1 “Radiation Protection of the Public and the Environment.” A reference person is a hypothetical, gender and age aggregation of human physical and physiological characteristics arrived at by international consensus for the purpose of standardizing radiation dose calculations. DOE O 458.1 states that compliance with the annual dose limit of 100 mrem (1 mSv) to a member of the public may be demonstrated by calculating the dose to the maximally exposed individual (MEI) or to a representative person. Historically, for dose compliance, SRS has used the MEI concept, which uses adult dose coefficients and adult male usage parameters. Beginning with the 2012 annual site environmental report, SRS will be using the representative person concept for dose compliance. The dose to a representative person will be based on 1) the SRS-specific reference person usage parameters at the 95th percentile of appropriate national or regional data, which are documented in this report, 2) the reference person (gender and age averaged) ingestion and inhalation dose coefficients provided in DOE Derived Concentration Technical Standard (DOE-STD-1196-2011), and 3) the external dose coefficients provided in the DC_PAK3 toolbox. The second purpose of this report is to develop SRS-specific derived concentration standards (DCSs) for all applicable food ingestion pathways, ground shine, and water submersion. The DCS is the concentration of a particular radionuclide in water, in air, or on the ground that results in a member of the public receiving 100 mrem (1 mSv) effective dose following continuous exposure for one year. In DOE-STD-1196-2011, DCSs were developed for the ingestion of water, inhalation of

  8. Determination of background concentrations of hydrochemical parameters and water quality assessment in the Akhuryan River Basin (Armenia)

    Science.gov (United States)

    Arpine, Hovhannisyan; Gayane, Shahnazaryan

    2016-08-01

    The determination of background values of hydrochemical parameters, to distinguish between natural concentration and anthropogenically-influenced concentrations, is highly relevant. In presented study, to estimate the background values of hydrochemical parameters in Akhuryan River Basin, log-normal probability functions on the hydrochemical parameters concentrations was applied. The study is carried out on the basis of hydrochemical data of surface water quality monitoring for the period of 2010-2013. This study highlights the usefulness of application of site-specific background concentrations for the evaluation, interpretation of surface water quality and for determination of pollution sources.

  9. Effect of methanolic leaf extract of Talinum triangulare (Jacq. willd. on biochemical parameters in diet induced dyslipidemia wistar rats

    Directory of Open Access Journals (Sweden)

    Olubukola Sinbad Olorunnisola

    2016-01-01

    Abbreviation used: Lipid peroxidation (MDA, (catalase (CAT, glutathione–S-transferase (GST, superoxide dismutase (SOD, glutathione (GSH, Thrombocytes indices (PLT, Red blood cell (RBC, Packed cell volume (PVC, Mean corpuscular hemoglobin(MCH, Mean corpuscular hemoglobin concentration (MCHC, Total glyceride (TG, Very low density lipoprotein (VLDL, Total cholesterol (TC, Low density lipoprotein (LDL, High density lipoprotein (HDL and 3-Hydroxy-3-methyl-glutaryl-CoA reductase(HMG-CoA.

  10. Mexico City ozone concentrations as a function of readily-available meteorological parameters

    Energy Technology Data Exchange (ETDEWEB)

    Brown, M.J.

    1994-03-01

    Daily maximum ozone concentrations measured at four sites within the Mexico City basin during the winter months are plotted as functions of different meteorological parameters that are routinely measured at surface stations. We found that ozone concentrations are most strongly correlated to the increase in daytime temperature and the maximum daytime wind speed. We also discovered that high ozone values at the sites in the southern end of the basin occur when winds come out of the northeast. In contrast, wind direction was found to be uncorrelated with high ozone values at the northern sites. From straightforward combinations of the meteorological variables, we derived some simple rules for estimating lower and upper bounds on the ozone concentration. Scatter in the data was too long to give significance to best-fit equations and statistics. Additionally, a small rawinsonde data set was used to investigate ozone`s dependence on boundary-layer height and near-surface temperature gradient. Results were inconclusive, however, due to the small size of the data set.

  11. Biochemical parameters of Spodoptera frugiperda (J. E. Smith) treated with citronella oil (Cymbopogon winterianus Jowitt ex Bor) and its influence on reproduction.

    Science.gov (United States)

    Silva, Cristiane Thalita Dos Santos; Wanderley-Teixeira, Valéria; Cunha, Franklin Magliano da; Oliveira, José Vargas de; Dutra, Kamilla de Andrade; Navarro, Daniela Maria do Amaral Ferraz; Teixeira, Álvaro Aguiar Coelho

    2016-05-01

    Spodoptera frugiperda is the principal corn pest in Brazil. Searches for new control methods that minimize the adverse effects of synthetic insecticides have initiated a resurgence of the use of botanical insecticides. Citronella oil (a product of Cymbopogon winterianus) is an effective repellent and insecticide. Thus, biochemical profile changes in oil-treated larvae and its influence on reproduction were assessed. Corn leaves dipped in a 50mg/mL concentration were offered to third instar larvae for 24h and assessed in sixth instar to estimate protein, lipid, sugar, and glycogen levels. Adult testes and ovarioles were collected for histological and histochemical analysis 24h after emergence. Number of eggs and hatching rate were also measured. Oil-treated larvae showed an increase in glycogen and a decrease in protein, lipid, and totals sugar content. Control testes exhibited connective tissue lining and cysts with abundant spermatozoids. However, intense peripheral vacuolation and neutral carbohydrates reduction occurred in oil-treated individuals. Control ovarioles showed normal morphologic characteristics. On the other hand, oil-treatment ovarioles showed follicular cell stratification and removal, reduced nurse cell development, reduced yolk quantity, a thinner conjunctiva sheath, and a reduction in proteins and neutral carbohydrates. Eggs derived from oil-treated pairs were unviable. Therefore, sub-lethal doses of citronella oil alters the biochemical profile of S. frugiperda larvae, causing damage to their reproductive histophysiology and results in diminished reproduction or reproductive failure.

  12. Evaluation of changes in blood hematological and biochemical parameters in response to Islamic fasting and regular physical activity in male and female subjects

    Directory of Open Access Journals (Sweden)

    Seyyed Reza Attarzadeh Hosseini

    2015-09-01

    Full Text Available Introduction: Fasting during Ramadan is a religious obligation for healthy adult Muslims. This study aimed to investigate the effects of Ramadan fasting and physical activity on blood hematological and biochemical parameters in male and female subjects. Methods: In this study, 50 healthy subjects were randomly assigned to four groups: male control fasting (MCF group (n=13, male training fasting (MTF group (n=13, female control fasting (FCF group (n=12, and female training fasting (FTF group (n=12, and were compared in two stages (before and after the intervention. For inter- and intra-group comparisons, a repeated measure ANOVA was applied. Results: The results showed a significant decline in body weight, body mass index, body fat percentage, and waist-to-hip ratio after Ramadan fasting, compared to the pre-Ramadan period. Also, significant changes were observed in red blood cell count and hematocrit level (P

  13. Physiological and biochemical parameters for identification of QTLs controlling the winter triticale drought tolerance at the seedling stage.

    Science.gov (United States)

    Hura, Tomasz; Hura, Katarzyna; Grzesiak, Stanisław

    2009-03-01

    The genetic map of the triticale is created on the basis of double-haploid (DH) lines, derived from F1 hybrids of a cross between the parental line Saka3006 and Modus. In order to localise drought resistance genes, it is necessary to find a phenotype feature which clearly differentiates between parental lines under drought stress conditions. With the future in mind, the aim of the presented studies was to analyse differences in the response to drought stress, between Saka3006 and Modus. Analyses of the water status of leaves, and the activity of the photosynthetic apparatus and protective mechanisms relating to the accumulation of phenolic compounds, were carried out. The studies were completed during the tillering phase. Statistically significant changes, between genotypes experiencing the drought period, were noticed for the osmotic potential, leaf water content, some parameters of chlorophyll fluorescence, and for phenolics and the ferulic acid content. On the basis of the studies, the Saka genotype can be considered drought resistant due to higher leaf water content caused, probably, by smaller hydraulic resistance relative to Modus. The activity of its photosynthetic apparatus during drought was higher than that for the Modus genotype. The high level of phenolic compounds, which can act as photoprotectors and free radical scavengers, was also maintained. All the mentioned parameters can represent the potential phenotype features, which allow the identification of resistance genes on the genetic map of the triticale, which is currently being created.

  14. Effects of dietary supplementation of fermented Ginkgo biloba L. residues on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets.

    Science.gov (United States)

    Zhou, Hao; Wang, Chengzhang; Ye, Jianzhong; Chen, Hongxia; Tao, Ran

    2015-08-01

    This study evaluated the effects of fermented Ginkgo biloba L. residues (FGBLR) on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets. Pigs were allotted to five dietary treatments, including negative control (NC: antibiotic free basal diet), positive control (PC) (NC + 30 mg apramycin/kg) and FGBLR-50, 100, 150 (NC + 50, 100, 150 g FGBLR/kg). Pigs in FGBLR-100 and PC treatments showed increased final body weight, average daily gain, gain:feed and apparent total tract digestibility of dry matter, N and gross energy (P < 0.05) compared with NC, FGBLR-50 and FGBLR-150 treatments, In addition, pigs fed with FGBLR-100 diet showed higher serum total protein, albumin, alkaline phosphatase, glucose, hemoglobin, total iron, total iron binding capacity, superoxide dismutase and glutathione superoxide dismutase levels, and lower serum blood urea nitrogen, malondialdehyde, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, triglyceride and total cholesterol levels than those fed with PC and NC diets (P < 0.05). Moreover, feeding FGBLR-100 could increase levels of immunoglobulin G (IgG), IgA and IgM, as well as lymphocyte transformation rates, ratio of CD4+ to CD8+ cells and proportions of CD2+, CD4+, B, major histocompatibility complex (MHC)-I and MHC-II cells, and can decrease proportion of CD8+ cells in blood of piglets compared with PC and NC groups (P < 0.05). These results indicate that dietary supplementation with 10% of FGBLR showed greatest beneficial effects on growth performance, nutrient digestibility, serum biochemical parameters and immune function in weaned piglets, which were superior to antibiotic supplemental diets.

  15. INFLUENCE OF SURFACE-ACTIVE RHAMNOLIPID BIOCOMPLEX AND ETHYLTHIOSULFANILATE ON GROWTH AND BIOCHEMICAL PARAMETERS OF PLANTS IN THE OIL CONTAMINATED SOIL

    Directory of Open Access Journals (Sweden)

    A. R. Banya

    2015-10-01

    Full Text Available The aim of the work was to study the influence of rhamnolipid biocomplex and ethylthiosulfanilate on field pea and sorghum plants when growing in petroleum contaminated soil. Plant seeds were treated with solutions of rhamnolipid biocomplex or ethylthiosulfanilate (0.01 g/l before planting and grown in containers with soil artificially contaminated with petroleum (5, 8 and 10%. Effect of rhamnolipid biocomplex and ethylthiosulfanilate was determined by the determination of growth (weight, length plants and biochemical parameters (content of photosynthetic pigments, hydrogen peroxide, malondialdehyde. The stimulating effect of rhamnolipid biocomplex and ethylthiosulfanilate on growth parameters of field pea was shown: the shoot mass has significantly increased on 39%, root mass – on 26% if compared with the control. For sorghum somewhat smaller increase of growth parameters was observed. Under the influence of rhamnolipid biocomplex and ethylthiosulfanilate the content of photosynthetic pigments in field pea and sorghum has also increased. It was determined that the action rhamnolipid biocomplex and ethylthiosulfanilate promoted the decrease of indicators of plant oxidative reactions if compared with control: the content of hydrogen peroxide – in average on 15% and 16%, malondialdehyde – on 13.5% and 16% respectively. The results of the study testify to the effectiveness of rhamnolipid biocomplex and ethylthiosulfanilate as growth stimulators for field pea and sorghum, as well as improvement of adaptive capability of plants to unfavorable conditions. It creates the prospects of their application as effective and ecologically safe substances for the intensification of contaminated soil phytoremediation.

  16. High sensitive C-reactive protein (hs-CRP level and biochemical parameters for prehypertension and prediabetes diagnosis

    Directory of Open Access Journals (Sweden)

    Yuttana Sudjaroen

    2015-01-01

    Full Text Available Context: Coronary atherosclerosis still presents one of the main causes of death. Efficacious prevention should focus on the early control of cardiovascular risk factors, including lipid profiles, which are unable early detect in subclinical cases. High-sensitive C-reactive protein (hs-CRP can prove to be an early cardiac risk predictor. Aims: 1 To compare hs-CRP levels between healthy volunteer with normal blood pressure and those with prehypertension, and 2 to use hs-CRP levels along with other risks to be a cardiac risk predictor. Setting and Design: This was a cross-sectional study for 6 months′ duration from January to June 2013 at Kudjab Hospital located in Udon Thani province, Thailand. Materials and Methods: Forty (40 healthy volunteers with prehypertension and 40 volunteers with normal blood pressure were included in the study. Both groups were similar in age range and sex. Twelve-hour (12-h fasting blood samples were collected from all the participants. Serum was assayed for hs-CRP and lipid profile. Results: All of parameters were statistically significant difference (P < 000.1. The hs-CRP level (6.27 ± 7.8 mg/L was elevated in the prehypertension group. The relative risk of hs-CRP for prehypertension was 6.3 with the odds ratio of 15.48, whereas the relative risk of lipid profiles for prehypertension prediction was only 1.28, with the odds ratio of 1.67. Statistical Analysis: SPSS version 11.0 using the unpaired t-test for comparing demographic data and blood parameters and risk prediction of hs-CRP and lipid profiles were calculated by relative risk with odds ratio [95% confidence interval (CI]. Conclusions: Hs-CRP is an early cardiac risk predictor even with normal lipid profile, and can help measure additional risk especially subclinical people such as prehypertension.

  17. Estimation of the indoor radon concentration by the analysis of relationship between the radon concentration and the meteorological parameters

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H. [Kyunghee Univ., Yongin (Korea, Republic of); Lee, W. K.; Jang, S. Y. [KAERI, Taejon (Korea, Republic of)

    2002-10-01

    Theoretical equation to estimate the indoor radon concentration can be derived from the multi-regression analysis method for the relationship between the indoor radon concentration and the meteorological variables such as the temperature, pressure and pressure difference at same indoor. The result of multi-regression analysis showed that the indoor radon concentration has been mostly influenced by the variation of the indoor temperature, while not so much influence by the indoor pressure difference. The indoor radon concentration theoretically estimated in this study agreed well with that really measured at same indoor within the range of statistical error. Therefore, it is possible to estimate the indoor rabon concentration by using the theoretical equation adopting the temperature and pressure difference at the same indoor.

  18. DNA Duplex Length and Salt Concentration Dependence of Enthalpy−Entropy Compensation Parameters for DNA Melting

    KAUST Repository

    Starikov, E. B.

    2009-08-20

    Systematical differential calorimetry experiments on DNA oligomers with different lengths and placed in water solutions with various added salt concentrations may, in principle, unravel important information about the structure and dynamics of the DNA and their water-counterion surrounding. With this in mind, to reinterpret the most recent results of calorimetric experiments on DNA oligomers of such a kind, the recent enthalpy-entropy compensation theory has been used. It is demonstrated that the application of the latter could enable direct estimation of thermodynamic parameters of the microphase transitions connected to the changes in DNA dynamical regimes versus the length of the biopolymers and the ionic strengths of their water solutions, and this calls for much more systematical experimental and theoretical studies in this field. © 2009 American Chemical Society.

  19. DNA duplex length and salt concentration dependence of enthalpy-entropy compensation parameters for DNA melting.

    Science.gov (United States)

    Starikov, E B; Nordén, Bengt

    2009-08-20

    Systematical differential calorimetry experiments on DNA oligomers with different lengths and placed in water solutions with various added salt concentrations may, in principle, unravel important information about the structure and dynamics of the DNA and their water-counterion surrounding. With this in mind, to reinterpret the most recent results of calorimetric experiments on DNA oligomers of such a kind, the recent enthalpy-entropy compensation theory has been used. It is demonstrated that the application of the latter could enable direct estimation of thermodynamic parameters of the microphase transitions connected to the changes in DNA dynamical regimes versus the length of the biopolymers and the ionic strengths of their water solutions, and this calls for much more systematical experimental and theoretical studies in this field.

  20. The Metabolic Effects of Consumption of Yellow Cassava (Manihot esculenta Crantz) on Some Biochemical Parameters in Experimental Rats.

    Science.gov (United States)

    Udeme, Nelson; Okafor, Polycarp; Eleazu, Chinedum

    2015-01-01

    The metabolism of yellow cassava (variety TMS 01/1368) was investigated in male albino rats fed a diet containing yellow cassava for 7 to 28 days. There were significant increases (P 0.05) in the serum total proteins of the experimental rats compared with the control. The experimental rats treated for 7, 14, 21, or 28 days exhibited body weight decreases of 5.11%, 11.10%, 19.16%, and 24.18%, respectively, whereas the control group showed 9.17% gain in body weight. Total and free cyanide concentrations were detected in the liver, kidney, and heart of most of the rats in both the experimental and control groups, except for free cyanide in the control group that was not detected. Metabolism of the yellow cassava variety in experimental rats was capable of exposing the animals to cyanide, underscoring the need for its proper processing before consumption by humans.

  1. Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.

    Science.gov (United States)

    Gielen, Fabrice; Buryska, Tomas; Van Vliet, Liisa; Butz, Maren; Damborsky, Jiri; Prokop, Zbynek; Hollfelder, Florian

    2015-01-06

    Analysis of concentration dependencies is key to the quantitative understanding of biological and chemical systems. In experimental tests involving concentration gradients such as inhibitor library screening, the number of data points and the ratio between the stock volume and the volume required in each test determine the quality and efficiency of the information gained. Titerplate assays are currently the most widely used format, even though they require microlitre volumes. Compartmentalization of reactions in pico- to nanoliter water-in-oil droplets in microfluidic devices provides a solution for massive volume reduction. This work addresses the challenge of producing microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination. We present a simple microcapillary technique able to generate such series of monodisperse water-in-oil droplets (with a frequency of up to 10 Hz) from a sample presented in an open well (e.g., a titerplate). Time-dependent variation of the well content results in microdroplets that represent time capsules of the composition of the source well. By preserving the spatial encoding of the droplets in tubing, each reactor is assigned an accurate concentration value. We used this approach to record kinetic time courses of the haloalkane dehalogenase DbjA and analyzed 150 combinations of enzyme/substrate/inhibitor in less than 5 min, resulting in conclusive Michaelis-Menten and inhibition curves. Avoiding chips and merely requiring two pumps, a magnetic plate with a stirrer, tubing, and a pipet tip, this easy-to-use device rivals the output of much more expensive liquid handling systems using a fraction (∼100-fold less) of the reagents consumed in microwell format.

  2. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Al-Okbi, S. Y.; Mohamed, D. A.; Donya, S. M.; Abd El Khalek, A. B.

    2011-07-01

    The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum) were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, {beta}-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory bio markers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures. (Author) 61 refs.

  3. Haematological, biochemical and inflammatory parameters in inactive Behçet's disease. Its association with red blood cell distribution width.

    Science.gov (United States)

    Vayá, Amparo; Rivera, Leonor; Todolí, José; Hernandez, Jose Luis; Laiz, Begoña; Ricart, José M

    2014-01-01

    Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complications; i.e.; thrombosis and posterior uveitis. A second aim was to analyse the possible correlation between RDW and both haematological and inflammatory parameters. Eighty-nine patients with BD (48 males/41 females) and 94 controls (49 males/45 females) were included in the study. Patients were in an inactive phase of the disease, showing only minimum activity. RDW was statistically higher in patients than in controls (14.02 ± 1.32 vs. 13.15 ± 0.75; p 0.05). RDW correlated negatively with haemoglobin, MCH and MCHC (p 0.05). The multivariate regression analysis revealed that haemoglobin and homocysteine were independent predictors of RDW (beta coefficient: -0.310; p = 0.003, beta coefficient: 0.379; p 14 was associated with neither thrombosis nor uveitis (p = 0.935; p = 0.553, respectively). Our results indicate that BD patients show increased RDW when compared with controls. This increase seems to be related with haematimetric indices and with homocysteine levels. Lack of correlation with inflammatory markers may be due to the fact that patients were in an inactive phase of the disease.

  4. Spatial and seasonal variability of temperature responses of biochemical photosynthesis parameters and leaf nitrogen content within a Pinus densiflora crown.

    Science.gov (United States)

    Han, Qingmin; Kawasaki, Tatsuro; Nakano, Takashi; Chiba, Yukihiro

    2004-07-01

    We measured seasonal variation in area-based nitrogen concentration (N), maximum rate of carboxylation (Vcmax) and maximum rate of electron transport (Jmax) in 1-year-old needles along four first-order branches within a Pinus densiflora Sieb. & Zucc. crown, and analyzed their relationships to growth irradiance and temperature. Each leaf light environment was expressed as a ratio of the monthly mean of daily integrated photosynthetically active irradiance (Iint) for the particular needle to Iint above the canopy (Irel). Needle N decreased in the upper crown during the development of new needles, whereas it remained fairly constant in the lower crown, reflecting differences between upper and lower crown needles in their contribution to the nitrogen of new needles. Gradients of N within the crown were correlated with Irel in all seasons (r2 = 0.40-0.78). Seasonal variation in N was weakly correlated with mean daily air minimum temperatures. Both Vcmax and Jmax showed seasonal variation in all first-order branches, and decreased to their lowest values in winter. The gradients of Vcmax and Jmax within the crown were not correlated with Irel in some seasons, but were correlated with changes in N in most months (r2 = 0.33-0.75), except in the winter. Furthermore, the regression slope of the relationship between N and Vcmax and the temperature response of Vcmax and Jmax exhibited seasonal variation.

  5. Postmortem biochemical behavior of giant squid (Dosidicus gigas) mantle muscle stored in ice and its relation with quality parameters.

    Science.gov (United States)

    Márquez-Ríos, E; Morán-Palacio, E F; Lugo-Sánchez, M E; Ocano-Higuera, V M; Pacheco-Aguilar, R

    2007-09-01

    Several freshness and spoilage indicators were monitored to characterize the postmortem biochemistry of giant squid (Dosidicus gigas) mantle muscle. Squid samples were obtained directly from the sea and kept at 0 degrees C during a 15-d storage period. Data at zero time were obtained from cryogenically frozen samples at time of capture. The adenosine 5'-triphosphate (ATP) degradation followed a different pattern as compared with that from fish species. ATP was almost completely depleted at 24-h postcatch from 6.54 to <1 micromol/g, while at the same time Hx was the predominant catabolite with a concentration of 4 mumol/g, reaching 6.85 micromol/g at day 15. K-value data followed a logarithmic pattern with time instead of a linear one, with no change after day 3, thus reducing its suitability as a freshness index. The coefficient Hx/AMP seems to be an adequate alternative for this purpose due to its constant increment with time. The high NH4Cl content in mantle muscle (461.3 +/- 24.5 mg of NH4(+)/100 g) derived from its physiological importance for the species compromises the use of the distillation step of the TVB-N analysis commonly used as a spoilage index. This fact explains why the initially high value of TVB-N detected in mantle muscle (243.7 mg N/100 g) did not correlate with the initial low TMA-N content (1.5 +/- 0.1 mg/100 g of muscle). The results suggested that under the experimental conditions the shelf life of squid exceeds 15 d.

  6. EFFECT OF FEEDING AMARANTH (AMARANTHUS ON AQUACULTURAL AND SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF AGE-2 CARP

    Directory of Open Access Journals (Sweden)

    R. Palamarchuk

    2016-06-01

    with artificial feeds of improved composition. This will allow reducing the cost of artificial feeds, obtaining higher growth rate, and improving physiological parameters of cultured carp.

  7. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

    Directory of Open Access Journals (Sweden)

    Donya, S. M.

    2011-09-01

    Full Text Available The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, β-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory biomarkers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures.El objetivo de la presente investigación fue encontrar extractos de alimentos vegetales y probióticos que puedan tener bioactividad hacía la inflamación crónica. Mezclas de tres extractos de alimentos vegetales conocidos por su riqueza en compuestos fenólicos, carotenoides y tocoferoles han sido preparadas. La actividad anti-inflamatoria de las diferentes mezclas y de bacterias probióticas (Bifidobacterium bifidum fue evaluada en artritis adyuvante en ratas. El efecto anti-inflamatorio, mecanismo de acción y salubridad de las tres mezclas y de Bifidobacterium bifidum ha sido estudiado mediante la medida del tamaño de la inflamación y la determinación de biomarcadores de inflamación y estrés oxidativo, del perfil de bacterias del colón y de parámetros citogen

  8. Growing Chlorella vulgaris in Photobioreactor by Continuous Process Using Concentrated Desalination: Effect of Dilution Rate on Biochemical Composition

    Directory of Open Access Journals (Sweden)

    Ângelo Paggi Matos

    2014-01-01

    Full Text Available Desalination wastewater, which contains large amount of salt waste, might lead to severely environmental pollution. This study evaluated the effect of dilution rate (0.1≤D≤0.3 day−1 on microalgal biomass productivity, lipid content, and fatty acid profile under steady-state condition of Chlorella vulgaris supplemented with concentrated desalination. Continuous culture was conducted for 55 days. Results show that the biomass productivity (Px varied from 57 to 126 mg L−1 d−1 (dry mass when the dilution rate ranged from 0.1 to 0.3 day−1. At lowest dilution rate (D=0.1 day−1, the continuous culture regime ensured the highest values of maximum biomass concentration (Xm=570±20 mL−1 and protein content (52%. Biomass lipid content was an increasing function of D. The most abundant fatty acids were the palmitic (25.3±0.6% at D=0.1 day−1 and the gamma-linolenic acid (23.5±0.1% at D=0.3 day−1 ones. These fatty acids present 14 to 18 carbons in the carbon chain, being mainly saturated and polyunsaturated, respectively. Overall, the results show that continuous culture is a powerful tool to investigate the cell growth kinetics and physiological behaviors of the algae growing on desalination wastewater.

  9. Relationship between dietary intake and use of protease inhibitors with anthropometric and biochemical parameters of lipodystrophy in people living with HIV

    Directory of Open Access Journals (Sweden)

    Tatiane Andreza Lima da Silva

    Full Text Available The lipodystrophy syndrome is characterized by redistribution of body fat and disorders of glicidic and lipid metabolism. Although its etiology is related to infection and drug therapy, there is little evidence regarding the nutritional disturbances on this association. This study aimed to assess the relationship between dietary intake and use of protease inhibitors (PIs with anthropometric and biochemical parameters in HIV positive patients. The study included 50 patients. A questionnaire about socioeconomic status, lifestyle and infection history was taken. In addition, it was conducted the evaluation of dietary intake (frequency questionnaire, anthropometric parameters (body mass index, waist circumference, triceps skinfold, corrected arm muscle area and biochemistry tests (glycemia and lipid profile. Only 37% of the sample was classified as "good food consumption", 54% were overweight or obese and 66% presented high waist circumference. The group with good food consumption had higher HDL-C (p=0.04 levels than the group with poor food consumption. Patients taking PIs presented VLDL-C (p=0.023 and triglycerides (p=0.024 levels significantly higher. These results indicated the necessity for continuous monitoring of HIV-positive patients and non-pharmacological interventions such as nutrition education and practice of physical exercises.

  10. Relationship between dietary intake and use of protease inhibitors with anthropometric and biochemical parameters of lipodystrophy in people living with hiv.

    Science.gov (United States)

    da Silva, Tatiane Andreza Lima; Barboza, Renata Rangel; de Andrade, Ricardo Dias; de Medeiros, Rafaela Catherine da Silva Cunha; de Medeiros, Jason Azevedo; de Souza, Hunaway Albuquerque Galvão; Leite, Lúcia Dantas; Dantas, Paulo Moreira Silva

    2014-10-01

    The lipodystrophy syndrome is characterized by redistribution of body fat and disorders of glicidic and lipid metabolism. Although its etiology is related to infection and drug therapy, there is little evidence regarding the nutritional disturbances on this association. This study aimed to assess the relationship between dietary intake and use of protease inhibitors (PIs) with anthropometric and biochemical parameters in HIV positive patients. The study included 50 patients. A questionnaire about socioeconomic status, lifestyle and infection history was taken. In addition, it was conducted the evaluation of dietary intake (frequency questionnaire), anthropometric parameters (body mass index, waist circumference, triceps skinfold, corrected arm muscle area) and biochemistry tests (glycemia and lipid profile). Only 37% of the sample was classified as "good food consumption", 54% were overweight or obese and 66% presented high waist circumference. The group with good food consumption had higher HDL-C (p=0.04) levels than the group with poor food consumption. Patients taking PIs presented VLDL-C (p=0.023) and triglycerides (p=0.024) levels significantly higher. These results indicated the necessity for continuous monitoring of HIV-positive patients and non-pharmacological interventions such as nutrition education and practice of physical exercises.

  11. The effects of concentrated system on the electrical parameters of GaInP/GaAs solar cell

    Science.gov (United States)

    Kınacı, Barış

    2017-02-01

    III-V concentrator solar cells are suitable materials in order to reduce the cost of photovoltaic electricity. By using Fresnel lens in concentrating photovoltaic technology is an effective way to entirely use the sunlight. In the present study, the research on the efficiency analysis of the GaInP/GaAs concentrated solar cell structure with AlGaAs tunnel junction was performed. The electrical output parameters of this structure were determined by concentrated system with Fresnel lens. The current-voltage measurements of concentrated solar cell were carried out at room temperature under both dark and air mass 1.5 global radiations. The parameters of GaInP/GaAs concentrated solar cell at 1 Sun and at 32 Suns are compared. It is obtained that the integration of the concentrated system on the solar cell structure improves the device performance by ≈7.5%.

  12. 牡蛎涂膜冻藏过程中生化指标的变化%Changes of Biochemical Parameters of Coating Oyster During Frozen Storage

    Institute of Scientific and Technical Information of China (English)

    张观科; 刘红英; 齐凤生

    2013-01-01

    Researching on Oyster . Oyster was storage with coating under the conditions in frozen environment, to explore its changes of pH,TBA,FFA,TVBN and Ca2+-ATPase during the processing. The results showed that: the storage method of biological preservative-coating is obviously better than the general group. By researching on changes of biochemical parameters during frozen Oyster storage, provide reference norms for frozen Oyster storage, long-transportation and sales.%  以牡蛎为研究对象,在冷冻条件下,采用涂膜技术进行保鲜贮藏试验,探讨其在此过程中pH、硫代巴比妥酸(TBA)、游离脂肪酸(FFA)、总挥发性盐基氮(TVBN)、Ca2+-ATPase值等生化指标的变化。结果表明,使用生物保鲜剂结合涂膜技术的复合保鲜效果明显好于空白处理,通过研究牡蛎生化指标的变化规律,为牡蛎冷冻保藏,长距离运输及销售等提供参考。

  13. Blood haematological and biochemical parameters in normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) maintained in isothermic and isonutritional conditions

    Institute of Scientific and Technical Information of China (English)

    Anthony Sabasthin; Venkataswamy Girish Kumar; Sumanta Nandi

    2012-01-01

    Objective:The present study was envisaged to examine the hematological and biochemical, parameters in three different groups of buffaloes (regularly cycling, pregnant and repeat breeding) maintained in isothermic and isonutritional conditions to establish the variations in blood and/or serum components in these groups.Methods:Blood samples were analyzed for hemoglobin, PCV, TLC, neutophil, lymphocyte, eosinophil, and monocyte count, glucose, total protein, albumin, globulin, urea and cholesterol.Results:The results revealed significantly lower haemoglobin and packed cell volume value in repeat breeding when compared to pregnant and regularly cycling animals. The WBC value significantly higher in repeat breeding when compared to regularly cycling animals. The average mean values of neutrophils, lymphocyte and eosinophils revealed a no significant difference in the neutrophils, lymphocyte and eosinophils in between the group. The mean values of monocytes revealed a significantly higher value in repeat breeding animals when compared to pregnant animals. The serum glucose, total protein, cholesterol and urea levels were significantly lower in repeat breeding compared to pregnant and regularly cycling animals. The levels of albumin and globulin revealed non-significant difference among the groups.Conclusions: A significant decrease in the hemoglobin, PCV, glucose, total protein, cholesterol and urea was observed in the repeat breeding animals when compared to pregnant and regularly cycling animals.

  14. Monitoring the effects of exposure to lead and cadmium in working and living environment through standard biochemical blood parameters and liver endonucleases activity

    Directory of Open Access Journals (Sweden)

    Nikolić Ružica S.

    2011-01-01

    Full Text Available Heavy metals as pollutants in the working and living environment are a serious health and environmental problem because they are toxic, non-biodegradable, accumulate in living systems and have a long half-life in soil. Sources of lead contamination are combustion products in the chemical industry and metallurgy, industrial waste water, landfills, traffic etc. Lead enters into the body via the food chain and drinking water. In the body lead is deposited in the liver, kidneys, brain and mineral tissues. Excretion of lead causes damage to the epithelial cells of certain organs. High level exposure to cadmium is usually the result of environmental pollution by human activities. Exposure to cadmium can lead to acute and chronic tissue damage of various organs, including liver and kidneys in humans and in animals. In this paper we analyzed the effects of lead and cadmium exposure, in working and living environment, on the model system of experimental animals, particularly the activity of certain liver enzymes, acid and alkaline DNase, and standard biochemical blood parameters. The study showed that lead and cadmium significantly affect the protein content, red blood cells, hemoglobin and hematocrit, and the activity of liver enzymes. This harmful effect of this toxic metal can be reduced by the supplements.

  15. Effect of acute exposure to nonylphenol on biochemical, hormonal, and hematological parameters and muscle tissues residues of Nile tilapia; Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Hager Tarek H. Ismail

    2016-06-01

    Full Text Available Aim: This study is aimed to evaluate some biochemical, hormonal, hematological, and histopathological changes in Nile tilapia, Oreochromis niloticus, after acute exposure to nonylphenol (NP. In addition to detection of NP residues in the fish, muscle tissues for human health concern. Materials and Methods: A total of 90 apparently healthy Nile tilapia, O. niloticus, were randomly divided into three equal groups; each containing 30 fish (three replicates. Groups 1 and 2 kept as a control and solvent control (acetone, respectively, and Group 3 exposed to NP at a dose level of 500 μg/L water for 7 successive days. Blood and tissue samples were collected 2 times randomly from each group after 7 days from fish exposure to NP and 10 days from exposure stopping. Results: Fish exposed to NP Group 3 showed anorexia, sluggish movement, erythema of the skin, areas of scales loss, and hemorrhagic ulcers in some areas of body region leading to exposing the viscera. Biochemical results revealed a significant increase in serum total proteins and globulins levels, a highly significant increase in serum alanine aminotransferase and aspartate aminotransferase activities, triglycerides, cholesterol, and creatinine levels, insignificant increase in serum uric acid level, and a highly significant decrease in serum testosterone and estradiol-β17 levels in Group 3 in compare with the control group. Histopathological finding confirms these results. While hematological results of the same group revealed a significant increase in red blood cells count and packed cell volume value, insignificant increase in hemoglobin concentration, leukopenia, lymphopenia, and monocytopenia in compared with the control group. All of these changes appeared after 7 days from fish exposure to NP. Most of these alterations returned toward the normal level after 10 days from stopping exposure to NP. NP residues detected in fish muscle tissues of Group 3 during exposure and after stopping

  16. Indoor air quality in the Karns research houses: baseline measurements and impact of indoor environmental parameters on formaldehyde concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Matthews, T. G.; Fung, K. W.; Tromberg, B. J.; Hawthorne, A. R.

    1985-12-01

    Baseline indoor air quality measurements, a nine-month radon study, and an environmental parameters study examining the impact of indoor temperature (T) and relative humidity (RH) levels on formaldehyde (CH2O) concentrations have been performed in three unoccupied research homes located in Karns, Tennessee. Inter-house comparison measurements of (1) CH2O concentration, (2) CH20 emission rates from primary CH20 emission sources, (3) radon and radon daughter concentrations, and (4) air exchange rates indicate that the three homes are similar. The results of the nine-month radon study indicate indoor concentrations consistently below the EPA recommended level of 4 pCi/L. Evidence was found that crawl-space concentrations may be reduced using heat pump systems whose outdoor units circulate fresh air through the crawl-spaoe. The modeled results of the environmental parameters study indicate approximate fourfold increases in CH20 concentrations from 0.07 to 0.27 ppm for seasonal T and RH conditions of 20°C, 30% RH and 29°C, 80% RH, respectively. Evaluation of these environmental parameters study data with steady-state CH2O concentration models developed from laboratory studies of the environmental dependence of CH2O emissions from particleboard underlayment indicate good correlations between the laboratory and field studies.

  17. Predicting the Activity Coefficients of Free-Solvent for Concentrated Globular Protein Solutions Using Independently Determined Physical Parameters

    OpenAIRE

    McBride, Devin W; Rodgers, Victor G. J.

    2013-01-01

    The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent...

  18. Estimation of the radon concentration in soil related to the environmental parameters by a modified Adaline neural network.

    Science.gov (United States)

    Negarestani, A; Setayeshi, S; Ghannadi-Maragheh, M; Akashe, B

    2003-02-01

    A new method based on adaptive linear neuron (Adaline) is used to estimate the radon concentration in soil associated with the environmental parameters. Analysis of the data obtained from a site in Thailand indicates that our proposed method is able to differentiate temporal variation of radon concentration related to the environmental parameters from those caused by phenomena in the earth (e.g. earthquake). The result also shows agreement between our method and another method based on impulse responses from multivariable time series (complex mathematical equations).

  19. Estimation of the radon concentration in soil related to the environmental parameters by a modified Adaline neural network

    Energy Technology Data Exchange (ETDEWEB)

    Negarestani, A. E-mail: anegar@seai.neda.net.ir; Setayeshi, S.; Ghannadi-Maragheh, M.; Akashe, B

    2003-02-01

    A new method based on adaptive linear neuron (Adaline) is used to estimate the radon concentration in soil associated with the environmental parameters. Analysis of the data obtained from a site in Thailand indicates that our proposed method is able to differentiate temporal variation of radon concentration related to the environmental parameters from those caused by phenomena in the earth (e.g. earthquake). The result also shows agreement between our method and another method based on impulse responses from multivariable time series (complex mathematical equations)

  20. Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers

    Institute of Scientific and Technical Information of China (English)

    Hua-li LI; Zong-jun LI; Zhong-shan WEI; Ting LIU; Xiao-zuo ZOU; Yong LIAO; Yu LUO

    2015-01-01

    This study investigates the long-term effects of oral tea polyphenols (TPs) and Lactobacillus brevis M8 (LB) on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1, 240 broiler chickens were selected to investigate the effects of 0.06 g/kg body weight (BW) TP and 1.0 ml/kg BW LB on broilers;in experiment 2, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of TP (0.03, 0.06, and 0.09 g/kg BW) combined with 1.0 ml/kg BW LB on broilers;in experiment 3, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of LB (0.5, 1.0, and 1.5 ml/kg BW) combined with 0.06 g/kg BW TP on broilers. The results showed that TP and LB affected serum bio-chemical parameters, and TP reduced serum cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) abundances in a dosage-dependent manner (P  目的:茶多酚和乳酸菌在动物营养上的作用已经得到广泛的验证,但是关于二者联合作用的研究鲜有报道。本文采用肉鸡作为试验模型,研究了茶多酚和乳酸菌联合灌喂对肉鸡血液生化、消化酶以及肠道细胞因子表达的影响。  创新点:本研究首次采用联合灌喂茶多酚和乳酸菌,探讨了二者联合作用对肉鸡的影响,并通过模拟生产,长期观察了茶多酚和乳酸菌对肉鸡的影响。  方法:对肉仔鸡灌喂不同浓度的茶多酚和乳酸菌,在第56和84天随机屠宰取样。收集血液检查血液生化指标,并测定消化酶活性。取肠道样品提取RNA,采用反转录聚合酶链反应(RT-PCR)检测细胞因子的表达以及相关信号通路的激活。  结论:长期灌喂茶多酚和乳酸菌改善了肉鸡脂质代谢、消化酶活性以及炎症反应,其机制可能是通过影响了NF-κB信号通路。

  1. Protective Effects of Vitamin C (Ascorbic Acid in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

    Directory of Open Access Journals (Sweden)

    Alireza AYOUBI

    2015-01-01

    Full Text Available The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42 were randomly assigned into7 groups: I healthy; II fed lead acetate only; III vitamin C administered only; IV diabetic; V diabetic rats administered by vitamin C; VI diabetic rats given lead acetate and VII diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

  2. Parâmetros clínicos, hematológicos, bioquímicos e hormonais de bovinos submetidos ao estresse calórico Clinical, hematological, biochemical, and hormonal parameters of cattle submitted to heat stress

    Directory of Open Access Journals (Sweden)

    F. Ferreira

    2009-08-01

    Full Text Available Caracterizaram-se as respostas clínicas, hematológicas, bioquímicas e hormonais de 71 bovinos F2 (½ Gir x ½ Holandês, 39 machos e 32 fêmeas, entre 14 e 20 meses de idade, submetidos a condições de termoneutralidade (22ºC a 70% de umidade relativa e ao estresse calórico (42ºC e 60% de umidade relativa. Os tratamentos foram realizados nas estações de verão e inverno em câmara bioclimática. Ao exame clínico, todos os animais apresentaram salivação e retração de globo ocular. Os animais apresentaram aumento do número de eritrócitos, concentração de hemoglobina, hematócrito, proteínas totais, ureia, creatinina, sódio, potássio, cloretos, cortisol, pH e densidade urinária e matéria seca das fezes no período de estresse calórico (P0,05. Concluiu-se que as variáveis hematológicas, bioquímicas e hormonais, bem como as características fenotípicas, revelaram-se importantes para caracterizar o estresse calórico em bovinos.Clinical, haemathological, biochemical, and hormonal parameters were characterized in 39 males and 32 females crossbred cattle (½ Holstein x ½ Gir, averaging 14 to 20 months of age, submitted to thermoneutrality (22ºC and 70% of RH and heat stress (42ºC and 60% RH in summer and winter in climatic chamber. At clinical examination, all animals presented salivation and ocular globe retraction. Under heat stress, animals increased erythrocytes count, hemoglobin total concentration, hematocrit, concentraction of total proteins, urea, creatinin, sodium, potassium, chlorides, cortisol, urinary pH and density, and dry matter of feces (P0.05. It was concluded that hematology, biochemical, and hormonal parameters are important to characterize the heat stress in cattle.

  3. Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio by Vitamin C and Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2015-12-01

    Full Text Available The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg/L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg/kg diet, vitamin C (1000 mg/kg diet or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA as well as biochemical parameters, including the activity of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, and acetylcholinesterase (AChE were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium-treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.

  4. The Effect of Cadmium on Physiological Indices, Growth Parameters and Nutrient Concentration in Tomato in Soilless Culture

    Directory of Open Access Journals (Sweden)

    Z. Ghasemi

    2010-08-01

    Full Text Available In order to determine the effects of cadmium on growth, physiological parameters, and nutritional elements’ concentration in tomato organs in a soilless system, an experiment was conducted in greenhouse of Isfahan Agricultural and Natural Resources Research Center in 2008 with a complete randomized block design with two treatments and 6 replications. The treatments were nutritional solutions of Hoagland with and without cadmium (20 µM. The measured indices included: RGR, NAR, RLGR, LAR, SLA, LWR, LWCA, length of the stem, fresh and dry weight of root and shoot, amount of sugar, photosynthetic pigments, and concentration of nitrogen, potassium, phosphorous, calcium, magnesium, iron, zinc, copper, manganese, and cadmium in root, stem, leaves and fruit. The results showed that cadmium (20 µM had significant effects on some measured growth indices. Growth parameters such as RGR, NAR, RLGR, Ls, RDW, RFW, SFW and SDW decreased 13.3, 19.1, 13.3, 18.8, 59.5, 58.7, 53.9 and 65.3%, respectively, and SLA increased 12.2%. But physiological parameters were not significantly affected by cadmium. The Cd concentration in all organs increased except fruit, compared with control which was Cd free. High cadmium concentration had significant effects on concentration of Mn and Cu in the root, Mn and P in the stem and Mn in the leaves. Concentration of Mn increased 5.3 and 87.6% in the leaves and stem, respectively, while it decreased 58.4% in the root. Concentration of P increased 33.3% and Cu increased 2times more in the stem. However, Ca concentration decreased 76.7% in the fruit, compared with the control. In conclusion, it is not recommended to grow tomato in the soils with high Cd concentration (polluted soil because of its negative effects on tomato growth.

  5. Correlations between Polyacetylene Concentrations in Carrot (Daucus carota L.) and Various Soil Parameters.

    Science.gov (United States)

    Kjellenberg, Lars; Johansson, Eva; Gustavsson, Karl-Erik; Granstedt, Artur; Olsson, Marie E

    2016-08-31

    This study assessed the concentrations of three falcarinol-type polyacetylenes (falcarinol, falcarindiol, falcarindiol-3-acetate) in carrots and the correlations between these and different soil traits. A total of 144 carrot samples, from three different harvests taken a single season, were analysed in terms of their polyacetylene concentrations and root development. On one of the harvesting occasions, 48 soil samples were also taken and analysed. The chemical composition of the soil was found to influence the concentrations of falcarinol-type polyacetylenes in carrots. When the total soil potassium level was 200 mg/100 g soil, the concentration of falcarindiol (FaDOH) in the carrot samples was 630 μg/g DW, but when carrots were grown in soil with a total potassium level of 300 mg/100 g soil, the FaDOH concentration in the carrots fell to 445 μg/g DW. Carrots grown in soils generally low in available phosphorus exhibited higher levels of falcarindiol if the soil was also low in available magnesium and calcium. The concentrations of polyacetylenes in carrots were positively correlated with total soil phosphorus level, but negatively correlated with total soil potassium level. Of the three polyacetylenes analysed, FaDOH concentrations were influenced most by changes in soil chemical composition.

  6. Correlations between Polyacetylene Concentrations in Carrot (Daucus carota L. and Various Soil Parameters

    Directory of Open Access Journals (Sweden)

    Lars Kjellenberg

    2016-08-01

    Full Text Available This study assessed the concentrations of three falcarinol-type polyacetylenes (falcarinol, falcarindiol, falcarindiol-3-acetate in carrots and the correlations between these and different soil traits. A total of 144 carrot samples, from three different harvests taken a single season, were analysed in terms of their polyacetylene concentrations and root development. On one of the harvesting occasions, 48 soil samples were also taken and analysed. The chemical composition of the soil was found to influence the concentrations of falcarinol-type polyacetylenes in carrots. When the total soil potassium level was 200 mg/100 g soil, the concentration of falcarindiol (FaDOH in the carrot samples was 630 μg/g DW, but when carrots were grown in soil with a total potassium level of 300 mg/100 g soil, the FaDOH concentration in the carrots fell to 445 μg/g DW. Carrots grown in soils generally low in available phosphorus exhibited higher levels of falcarindiol if the soil was also low in available magnesium and calcium. The concentrations of polyacetylenes in carrots were positively correlated with total soil phosphorus level, but negatively correlated with total soil potassium level. Of the three polyacetylenes analysed, FaDOH concentrations were influenced most by changes in soil chemical composition.

  7. Correlations between Polyacetylene Concentrations in Carrot (Daucus carota L.) and Various Soil Parameters

    OpenAIRE

    Lars Kjellenberg; Eva Johansson; Karl-Erik Gustavsson; Artur Granstedt; Marie E. Olsson

    2016-01-01

    This study assessed the concentrations of three falcarinol-type polyacetylenes (falcarinol, falcarindiol, falcarindiol-3-acetate) in carrots and the correlations between these and different soil traits. A total of 144 carrot samples, from three different harvests taken a single season, were analysed in terms of their polyacetylene concentrations and root development. On one of the harvesting occasions, 48 soil samples were also taken and analysed. The chemical composition of the soil was foun...

  8. concentration

    Directory of Open Access Journals (Sweden)

    Seth F. Oppenheimer

    1999-01-01

    Full Text Available We consider a model for biochemical oxygen demand (BOD in a semi-infinite river where the BOD is prescribed by a time varying function at the left endpoint. That is, we study the problem with a time varying boundary loading. We obtain the well-posedness for the model when the boundary loading is smooth in time. We also obtain various qualitative results such as ordering, positivity, and boundedness. Of greatest interest, we show that a periodic loading function admits a unique asymptotically attracting periodic solution. For non-smooth loading functions, we obtain weak solutions. Finally, for certain special cases, we show how to obtain explicit solutions in the form of infinite series.

  9. Predicting the activity coefficients of free-solvent for concentrated globular protein solutions using independently determined physical parameters.

    Directory of Open Access Journals (Sweden)

    Devin W McBride

    Full Text Available The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent model, which has previously been shown to provide excellent prediction of the osmotic pressure of concentrated and crowded globular proteins in aqueous solutions up to near-saturation concentrations. Thus, this model uses only the independently determined, physically realizable quantities: mole fraction, solvent accessible surface area, and ion binding, in its prediction. Predictions are presented for the activity coefficients of free-solvent for near-saturated protein solutions containing either bovine serum albumin or hemoglobin. As a verification step, the predictability of the model for the activity coefficient of sucrose solutions was evaluated. The predicted activity coefficients of free-solvent are compared to the calculated activity coefficients of free-solvent based on osmotic pressure data. It is observed that the predicted activity coefficients are increasingly dependent on the solute-solvent parameters as the protein concentration increases to near-saturation concentrations.

  10. Predicting the activity coefficients of free-solvent for concentrated globular protein solutions using independently determined physical parameters.

    Science.gov (United States)

    McBride, Devin W; Rodgers, Victor G J

    2013-01-01

    The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent model, which has previously been shown to provide excellent prediction of the osmotic pressure of concentrated and crowded globular proteins in aqueous solutions up to near-saturation concentrations. Thus, this model uses only the independently determined, physically realizable quantities: mole fraction, solvent accessible surface area, and ion binding, in its prediction. Predictions are presented for the activity coefficients of free-solvent for near-saturated protein solutions containing either bovine serum albumin or hemoglobin. As a verification step, the predictability of the model for the activity coefficient of sucrose solutions was evaluated. The predicted activity coefficients of free-solvent are compared to the calculated activity coefficients of free-solvent based on osmotic pressure data. It is observed that the predicted activity coefficients are increasingly dependent on the solute-solvent parameters as the protein concentration increases to near-saturation concentrations.

  11. Effects of temperature and discharge parameters on ozone concentration of negative corona discharge

    Institute of Scientific and Technical Information of China (English)

    WANG Hai-jun; SHENG Lian-xi; XU De-xuan

    2003-01-01

    The relationship of the ozone generation and the heating power, corona wire surface temperature, discharge electrode and netting electrode was studied during the negative corona discharge. The experimental results showed that the ozone concentration reduced with applied voltage decrease. With heating power increase, the ozone concentration of unit current decreased at exponential rate and it almost change no longer over 0.40 W. Under given temperature, the lower the applied voltage was, the smaller the ozone concentration was; while under given applied voltages, only over 11 kV could decrease with the surface temperature increase. The ozone concentration decreased with the lengthening of corona wire, and could reduced to 10 ppb under experimental condition of 14.2 kV; it also decreased with the shortening of wire diameter,and could decrease 67% at best in the given condition. Moreover, it decreased with the increasing size of netting electrode mesh. At the anion current of 1.65 μA, the ozone concentration of 5.0 × 5.0 cm2 is only 41% of that of 3.3 × 3.3 cm2 .

  12. The physiological and biochemical responses of a medicinal plant (Salvia miltiorrhiza L. to stress caused by various concentrations of NaCl.

    Directory of Open Access Journals (Sweden)

    Zhao Gengmao

    Full Text Available Salvia miltiorrhiza, which is commonly known as Danshen, is a traditional Chinese herbal medicine. To illustrate its physiological and biochemical responses to salt stress and to evaluate the feasibility of cultivating this plant in saline coastal soils, a factorial experiment under hydroponic conditions was arranged on the basis of a completely randomised design with three replications. Five salinity treatments (0, 25, 50, 75 and 100 mM NaCl were employed in this experiment. The results showed that salinity treatments of <100 mM NaCl did not affect the growth of Salvia miltiorrhiza in a morphological sense, but significantly inhibit the accumulation of dry matter. Salinity treatments significantly decreased the Chl-b content but caused a negligible change in the Chl-a content, leading to a conspicuous overall decrease in the T-Chl content. The Na(+ content significantly increased with increasing hydroponic salinity but the K(+ and Ca(2+ contents were reversed, indicating that a high level of external Na(+ resulted in a decrease in both K(+ and Ca(2+ concentrations in the organs of Salvia miltiorrhiza. Salt stress significantly decreased the superoxide dismutase (SOD activity of Salvia miltiorrhiza leaves in comparison with that of the control. On the contrary, the catalase (CAT activity in the leaves markedly increased with the increasing salinity of the hydroponic solution. Moreover, the soluble sugar and protein contents in Salvia miltiorrhiza leaves dramatically increased with the increasing salinity of the hydroponic solution. These results suggested that antioxidant enzymes and osmolytes are partially involved in the adaptive response to salt stress in Salvia miltiorrhiza, thereby maintaining better plant growth under saline conditions.

  13. Determination of effect factor for effective parameter on saccharification of lignocellulosic material by concentrated acid

    Science.gov (United States)

    Aghili, Sina; Nodeh, Ali Arasteh

    2015-12-01

    Tamarisk usage as a new group of lignocelluloses material to produce fermentable sugars in bio ethanol process was studied. The overall aim of this work was to establish the optimum condition for acid hydrolysis of this new material and a mathematical model predicting glucose release as a function of operation variable. Sulfuric acid concentration in the range of 20 to 60%(w/w), process temperature between 60 to 95oC, hydrolysis time from 120 to 240 min and solid content 5,10,15%(w/w) were used as hydrolysis conditions. HPLC was used to analysis of the product. This analysis indicated that glucose was the main fermentable sugar and was increase with time, temperature and solid content and acid concentration was a parabola influence in glucose production. The process was modeled by a quadratic equation. Curve study and model were found that 42% acid concentration, 15 % solid content and 90oC were optimum condition.

  14. Influence of smoking parameters on the concentration of polycyclic aromatic hydrocarbons (PAHs) in Danish smoked fish

    DEFF Research Database (Denmark)

    Duedahl-Olesen, Lene; Christensen, J. H.; Højgård, A.

    2010-01-01

    ), solid-phase extraction (silica gel), and gas chromatography-mass spectrometry analysis. The sum concentration of 25 PAHs (Sigma PAH25) was highest in smoked herring (n = 3) and mackerel fillets (n = 13), with an average concentration of 320 and 235 mu g kg-1, respectively. Lowest average Sigma PAH25......A new method for the analysis of 25 polycyclic aromatic hydrocarbon (PAH) compounds in fish was developed, validated, and used for the quantification of PAHs in 180 industrially smoked fish products. The method included pressurized liquid extraction, gel-permeation chromatography (Bio-beads S-X3...

  15. Ethanol extract of mango (Mangifera indica L.) peel inhibits α-amylase and α-glucosidase activities, and ameliorates diabetes related biochemical parameters in streptozotocin (STZ)-induced diabetic rats

    OpenAIRE

    Gondi, Mahendranath; U. J. S. Prasada Rao

    2015-01-01

    Peel is a major by-product during processing of mango fruit into pulp. Recent report indicates that the whole peel powder ameliorated diabetes. In the present study, ethanolic extract of mango peel was analysed for its bioactive compounds, evaluated for α-amylase and α-glucosidase inhibitory properties, oral glucose tolerance test, antioxidant properties, plasma insulin level and biochemical parameters related to diabetes. In addition to gallic and protocatechuic acids, the extract also had c...

  16. Temperature insensitive prediction of glucose concentration in turbid medium using multivariable calibration based on external parameter orthogonalization

    Science.gov (United States)

    Han, Tongshuai; Zhang, Ziyang; Sun, Cuiying; Guo, Chao; Sun, Di; Liu, Jin

    2016-10-01

    The measurement accuracy of non-invasive blood glucose concentration (BGC) sensing with near-infrared spectroscopy is easily affected by the temperature variation in tissue because it would induce an unacceptable spectrum variation and the consequent prediction deviation. We use a multivariable correction method based on external parameter orthogonalization (EPO) to calibrate the spectral data recorded at different temperature values to reduce the spectral variation. The tested medium is a kind of tissue phantom, the Intralipid aqueous solution. The calibration uses a projection matrix to get the orthogonal spectral space to the variable of external parameter, i.e. temperature, and then the useful spectral information relative to glucose concentration has been reserved. Even more, training the projection matrix can be separated to building the calibration matrix for the prediction of glucose concentration as it only uses the representative samples' data with temperature variation. The method presents a lower complexity than modeling a robust prediction matrix, which can be built from comprehensive spectral data involved the all variables both of BGC and temperature. In our test, the calibrated spectra with the same glucose concentration but different temperature values show a significantly improved repeatability. And then the glucose concentration prediction results show a lower root mean squared error of prediction (RMSEP) than that using the robust calibration model, which has considered the two variables. We also discuss the rationality of the representative samples chosen by EPO. This research may be referenced to the temperature calibration for in vivo BGC sensing.

  17. The slope parameter of concentration-response curves used as a touchstone for the existence of spare receptors.

    Science.gov (United States)

    Agneter, E; Singer, E A; Sauermann, W; Feuerstein, T J

    1997-09-01

    The present work was stimulated by findings of a large reserve of presynaptic alpha2-autoreceptors in rat neocortex by different investigators and our own group, using classical models of receptor agonism. The mathematical background of these classical models seems erroneous since the asymmetry that spare receptors introduce into concentration-response curves is not considered appropriately. This asymmetry leads to a steepening of curve fits based on the logistic function. Therefore, the slope parameter c of a logistically fitted concentration-response curve can be used as a touchstone for the existence of spare receptors. Spare receptors induce a c > 1. Concentration-response data of the alpha2-autoreceptor-mediated inhibition of evoked [3H]-noradrenaline release in rat neocortex slices were re-analysed. The estimates of the slope parameter c of logistically fitted concentration-response curves obtained after treatment of rats with either vehicle or N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) to achieve an irreversible inactivation of alpha2-autoreceptors, were not compatible with the existence of a large receptor reserve. A model for nonlinear regression analysis developed under the a priori assumption of spare receptors confirmed the absence of spare receptors. Evaluation methods which neglect the alteration of the geometrical form of concentration-response curves due to non-proportionality between receptor occupation and relative response do not seem appropriate to quantify spare receptors. These methods may detect spare receptors where they do not exist.

  18. POLYMORPHISM AT THE ADH AND ALPHA-GPDH LOCI IN DROSOPHILA-MELANOGASTER - EFFECTS OF REARING TEMPERATURE ON DEVELOPMENTAL RATE, BODY-WEIGHT, AND SOME BIOCHEMICAL PARAMETERS

    NARCIS (Netherlands)

    OUDMAN, L; VANDELDEN, W; KAMPING, A; BIJLSMA, R

    1991-01-01

    The role of developmental time in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster, and the relationship with weight and some biochemical characters, were investigated. Experimental strains were constructed with different combinations of Adh and alpha-Gpdh all

  19. Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice: Part II: Impact on specific chemical and biochemical quality parameters

    NARCIS (Netherlands)

    Vervoort, L.; Plancken, van der I.; Grauwet, T.; Timmermans, R.A.H.; Mastwijk, H.C.; Matser, A.M.; Hendrickx, M.E.; Loey, van A.

    2011-01-01

    The impact of thermal, high pressure (HP) and pulsed electric field (PEF) processing for mild pasteurization of orange juice was compared on a fair basis, using processing conditions leading to an equivalent degree of microbial inactivation. Examining the effect on specific chemical and biochemical

  20. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A new analytical method for the determination of urea-urease system based on biochemical reaction heat induced laser beam deflection is presented in this paper. With the method, the Michaelis constant (Km) of urease and apparent inhibition constant (Ki) of some metal ion inhibitors were measured respectively. This method was also used for the quantitative determination of metal ions with satisfactory result.

  1. A Dependence of the Electronuclear System Parameters on the Component Concentration in Fuel MOX

    CERN Document Server

    Barashenkov, V S

    2001-01-01

    A dependence of the parameters of a electronuclear system with U-Pu fuel MOX on the relative share of plutonium and its isotope ^{240}Pu in fuel is investigated by means of mathematical modelling. As an example, we consider an experimental system with a heat power 10-20 kW designed in Dubna on the basis of the 660 MeV proton phasotron. The 2 % admixture of ^{240}Pu decreases the value of the neutron multiplication coefficient from 0.95 down to 0.90, neutron yield and heat power are diminished almost twice. Such a decrease can be compensated by the increase of Plutonium share in MOX from 25 up to 27 %.

  2. Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples.

    Science.gov (United States)

    Levitas, E; Lunenfeld, E; Weisz, N; Friger, M; Potashnik, G

    2007-04-01

    This study evaluates retrospectively the relationship between age and semen parameters among men with normal sperm concentration. It was based on computerized data and performed in an Academic Fertility and IVF Unit. Six thousand and twenty-two semen samples with sperm concentrations of >or=20 x 10(6) ml(-1) were examined according to WHO criteria and analysed in relation to patients' age. For each age group, mean values +/- SD of semen volume, sperm concentration, percentage of motile spermatozoa, normal morphology, acrosome index, total sperm count/ejaculate, total motile sperm count/ejaculate and sexual abstinence duration were examined. A peak semen volume of 3.51 +/- 1.76 ml(-1) was observed at age >or=30 to age >or=55 years (PSperm motility was found to be inversely related to age with peak motility of 44.39 +/- 20.69% at age age >or=55 years (Psperm, between values of 103.34 +/- 107 x 10(6) at age >or=30 to age >55 years. A statistically significant and inverse relationship was observed between semen volume, sperm quality and patient age, in spite of prolonged sexual abstinence duration. Top sperm parameters were observed at age >or=30 to sperm parameters occurred after the age of 55 years.

  3. A hepcidina como parâmetro bioquímico na avaliação da anemia por deficiência de ferro Hepcidin as a biochemical parameter for the assessment of iron deficiency anemia

    Directory of Open Access Journals (Sweden)

    Andrea dos Reis Lemos

    2010-01-01

    parameter for assessment of iron deficiency anemia is reported in this review. A literature review was carried out in the databases PubMed and LILACS, between 2006-2010, assessing hepcidin as a parameter for the regulation of iron metabolism. During this period 35 studies were published in international journals and one study in a Brazilian journal. The production of hepcidin is homeostatically regulated by anemia and hypoxia. When oxygen supply is inadequate the level of hepcidin decreases. Therefore, more iron from the diet and from the stock of macrophages and hepatocytes becomes available. Hepcidin links to ferroportin, regulating iron release to plasma. When the hepcidin concentrations are low, the molecules of ferroportin are exposed on the plasmatic membrane and release iron. When hepcidin levels increase, hepcidin binds to molecules of ferroportin inducing its internalization and degradation and iron release gradually decreases. Apparently, development of diagnosis and therapy for anemia based on the parameter hepcidin may provide a more effective approach. Large-studies are needed to demonstrate the importance of hepcidin for the differential diagnosis of anemia, including sample protocols for analysis, with standards similar to those used in other biochemical evaluations, as well as the definition of cut-off points for the plasma and urinary expression of this peptide.

  4. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea.

    Science.gov (United States)

    Tomaszewska, Ewa; Winiarska-Mieczan, Anna; Dobrowolski, Piotr

    2015-01-01

    Rats were used to check whether regular consumption of black, red, or white tea would have a protective effect similar to the action of green tea on the intestine and liver in the case of exposure to Cd and Pb within the limits of human environmental exposure to these elements. Rats at the age of 6 weeks were divided into the control and four groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. Their diet (except the control) was mixed with 7 mg Cd/kg and 50mg Pb/kg. The experiment lasted 12 weeks. The effects of administration of tea in Cd- and Pb-poisoned rats on plasma biochemical parameters and the jejunal epithelium and liver were determined. The highest body mass was found in the GT group. The highest hemoglobin and Fe concentrations were in the control and GT groups. The highest activity of AST was in groups poisoned with Cd and Pb independently on supplementation. The highest ALT activity was in BT and RT groups with lower content of polifenoles. Pb and Cd disturbed the liver leading to necrosis and fatty degenerative changes, and a loss of normal architecture of the hepatocytes. Rats from the GT group had the highest cell proliferation rate in intestinal glands and the largest absorptive surface. Black, red, and white tea exerted a varied impact on the histological structure and innervation of the small intestine wall as well as on the absorptive function of small intestine mucosa in rats poisoned with Pb and Cd than green tea. On the other hand, taking into account the number of apoptotic cells, the effect of the teas was the same. Moreover, it is clear that long term exposure to Cd and Pb contamination causes toxic effect in the liver.

  5. Effect of concentration of dispersed organic matter on optical maturity parameters: Interlaboratory results of the organic matter concentration working group of the ICCP.

    Science.gov (United States)

    Mendonca, Filho J.G.; Araujo, C.V.; Borrego, A.G.; Cook, A.; Flores, D.; Hackley, P.; Hower, J.C.; Kern, M.L.; Kommeren, K.; Kus, J.; Mastalerz, Maria; Mendonca, J.O.; Menezes, T.R.; Newman, J.; Ranasinghe, P.; Souza, I.V.A.F.; Suarez-Ruiz, I.; Ujiie, Y.

    2010-01-01

    The main objective of this work was to study the effect of the kerogen isolation procedures on maturity parameters of organic matter using optical microscopes. This work represents the results of the Organic Matter Concentration Working Group (OMCWG) of the International Committee for Coal and Organic Petrology (ICCP) during the years 2008 and 2009. Four samples have been analysed covering a range of maturity (low and moderate) and terrestrial and marine geological settings. The analyses comprise random vitrinite reflectance measured on both kerogen concentrate and whole rock mounts and fluorescence spectra taken on alginite. Eighteen participants from twelve laboratories from all over the world performed the analyses. Samples of continental settings contained enough vitrinite for participants to record around 50 measurements whereas fewer readings were taken on samples from marine setting. The scatter of results was also larger in the samples of marine origin. Similar vitrinite reflectance values were in general recorded in the whole rock and in the kerogen concentrate. The small deviations of the trend cannot be attributed to the acid treatment involved in kerogen isolation but to reasons related to components identification or to the difficulty to achieve a good polish of samples with high mineral matter content. In samples difficult to polish, vitrinite reflectance was measured on whole rock tended to be lower. The presence or absence of rock fabric affected the selection of the vitrinite population for measurement and this also had an influence in the average value reported and in the scatter of the results. Slightly lower standard deviations were reported for the analyses run on kerogen concentrates. Considering the spectral fluorescence results, it was observed that the ??max presents a shift to higher wavelengths in the kerogen concentrate sample in comparison to the whole-rock sample, thus revealing an influence of preparation methods (acid treatment) on

  6. The relationship between some meteorological parameters and the tropospheric concentrations of ozone in the urban area of Belgrade

    Directory of Open Access Journals (Sweden)

    DRAGAN M. MARKOVIC

    2005-12-01

    Full Text Available During the period between June and December 2002, the concentrations of ozone in the air at 4 measuring sites in Belgrade were measured. The measuring periods varied from 10 days to several weeks. Themaximalmeasured daily concentrations of ozone ranged from 19 ppbv (23 December 2002 to 118 ppbv (23 June 2002. Ozone concentrations higher than, or equal to 90 ppbv were registered at threemeasuring sites. It was shown that at measuring sites characterized as urban, maximal O3 concentrations equal to, or higher than 90 ppbv occurred at high temperatures (higher than 30 oC and low wind speeds (mostly from the north. The measured ozone concentrations mostly showed characteristics usual for a daily photochemical ozone cycle, excluding the specificities influenced by the measuring site itself. Ozone transport was recorded at increased wind speeds, primarily from south-easterly directions. On the basis of he correlations between ozone concentration and the corresponding meteorological parameters, a validation of the measuring sites was performed from the aspect of their representativeness for the measurements.

  7. Deoxynivalenol in chicken feed alters the vaccinal immune response and clinical biochemical serum parameters but not the intestinal and carcass characteristics.

    Science.gov (United States)

    Ghareeb, K; Awad, W A; Zebeli, Q; Böhm, J

    2016-02-01

    This study was conducted to investigate the impacts of deoxynivalenol (DON) feeding either alone or in combination with a microbial feed additive (MFA) on the immune response to a viral vaccine and serum clinical chemical parameters. Forty 1-day-old boiler chicks were weighed and randomly divided into four groups, 10 birds in each group: (i) control group fed with basal diet; (ii) DON group fed with basal diet artificially contaminated with 10 mg DON/kg feed; (iii) DON + MFA group fed with basal diet contaminated with 10 mg DON/kg feed and supplemented with 2.5 kg of MFA/ton feed; and (iv) MFA group fed with basal diet supplemented with 2.5 kg of MFA/ton feed. At 35 days of age, birds were slaughtered and blood was collected for investigating the antibody titre against infectious bronchitis virus (IBV) and clinical chemical parameters. The results showed that DON reduced (p = 0.032) the titre against IBV, decreased (p = 0.005) the level of alanine transaminase (ALT) (4.2 ± 0.5 U/l) compared with control birds (6.4 ± 0.5 U/l), increased (p = 0.002) the serum cholesterol concentration (144 ± 6 mg/dl) compared with their control counterparts (123 ± 5 mg/dl) and increased (p = 0.074) the amount of circulating triglycerides (62.25 ± 7.50 mg/dl) compared with controls (39.55 ± 4.74). These results indicate that dietary DON altered the humoral immune response to viral vaccine and affected the serum clinical biochemistry. However, DON in combination with MFA did not affect serum IBV titre. Taken together, DON in the feed of broilers produced an impairment of the success of IBV vaccine and affected the health of birds.

  8. Global Mass Flux Solutions from GRACE: A Comparison of Parameter Estimation Strategies - Mass Concentrations Versus Stokes Coefficients

    Science.gov (United States)

    Rowlands, D. D.; Luthcke, S. B.; McCarthy J. J.; Klosko, S. M.; Chinn, D. S.; Lemoine, F. G.; Boy, J.-P.; Sabaka, T. J.

    2010-01-01

    The differences between mass concentration (mas con) parameters and standard Stokes coefficient parameters in the recovery of gravity infonnation from gravity recovery and climate experiment (GRACE) intersatellite K-band range rate data are investigated. First, mascons are decomposed into their Stokes coefficient representations to gauge the range of solutions available using each of the two types of parameters. Next, a direct comparison is made between two time series of unconstrained gravity solutions, one based on a set of global equal area mascon parameters (equivalent to 4deg x 4deg at the equator), and the other based on standard Stokes coefficients with each time series using the same fundamental processing of the GRACE tracking data. It is shown that in unconstrained solutions, the type of gravity parameter being estimated does not qualitatively affect the estimated gravity field. It is also shown that many of the differences in mass flux derivations from GRACE gravity solutions arise from the type of smoothing being used and that the type of smoothing that can be embedded in mas con solutions has distinct advantages over postsolution smoothing. Finally, a 1 year time series based on global 2deg equal area mascons estimated every 10 days is presented.

  9. Effect of environmental parameters on pathogen and faecal indicator organism concentrations within an urban estuary

    Science.gov (United States)

    Henry, Rebekah; Schang, Christelle; Kolotelo, Peter; Coleman, Rhys; Rooney, Graham; Schmidt, Jonathan; Deletic, Ana; McCarthy, David T.

    2016-06-01

    Current World Health Organisation figures estimate that ∼2.5 million deaths per year result from recreational contact with contaminated water sources. Concerns about quantitative risk assessments of waterways using faecal indicator organisms (FIOs) as surrogates to infer pathogenic risk currently exist. In Melbourne, Australia, the Yarra River has come under public scrutiny due to perceived public health risks associated with aquatic recreation; a characteristic shared with urban estuaries worldwide. A 10-month study of the Yarra estuary investigated the processes that affect FIOs and pathogens within this system. A total of 74 samples were collected from three estuarine and two upstream, freshwater, locations under different climatic and hydrological conditions, and the levels of Escherichia coli, enterococci, Clostridium perfringens, fRNA coliphages, Campylobacter spp. Cryptosporidium oocysts, Giardia cysts, adenoviruses, and enteroviruses were monitored. Reference pathogenic bacteria, protozoa, and viruses were detected in 81%, 19%, and 8% of samples, respectively. Variations in FIO concentrations were found to be associated with changes in specific climatic and hydrological variables including: temperature, flow, humidity and rainfall. In contrast, pathogen levels remained unaffected by all variables investigated. Limitations of current national and international culture-based standard methods may have played a significant role in limiting the identification of correlative relationships The data demonstrate the differences between FIOs and microbial pathogens in terms of sources, sinks, and survival processes within an urban estuary and provide further evidence of the inadequacy of FIO inclusion in the development of worldwide regulatory water quality criteria and risk assessment models.

  10. Genetic parameters for concentrations of minerals in longissimus muscle and their associations with palatability traits in Angus cattle.

    Science.gov (United States)

    Mateescu, R G; Garmyn, A J; Tait, R G; Duan, Q; Liu, Q; Mayes, M S; Garrick, D J; Van Eenennaam, A L; Vanoverbeke, D L; Hilton, G G; Beitz, D C; Reecy, J M

    2013-03-01

    The objective of this study was to estimate genetic parameters for concentrations of minerals in LM and to evaluate their associations with beef palatability traits. Samples of LM from 2,285 Angus cattle were obtained and fabricated into steaks for analysis of mineral concentrations and for trained sensory panel assessments. Nine minerals, including calcium, copper, iron, magnesium, manganese, phosphorus, potassium, sodium, and zinc, were quantified. Restricted maximum likelihood procedures were used to obtain estimates of variance and covariance components under a multiple-trait animal model. Estimates of heritability for mineral concentrations in LM varied from 0.01 to 0.54. Iron and sodium were highly and moderately heritable, respectively, whereas the other minerals were lowly heritable except for calcium, copper, and manganese, which exhibited no genetic variation. Strong positive genetic correlations existed between iron and zinc (0.49, P minerals (P > 0.05). Beefy flavor was positively associated with calcium, iron, and zinc and negatively associated with sodium concentration, whereas a painty or fishy flavor was positively associated with sodium and negatively associated with calcium and potassium concentrations (P < 0.05). Beef is a major contributor of iron and zinc in the human diet, and these results demonstrate sufficient genetic variation for these traits to be improved through marker-assisted selection programs without compromising beef palatability.

  11. 禁欲时间对精子参数及精浆生化指标的影响%Effects of sexual abstinence on sperm parameters and seminal plasma biochemical indicators

    Institute of Scientific and Technical Information of China (English)

    李付彪; 张明; 封建军; 刘祥印; 刘睿智

    2008-01-01

    Objective To study the influences of the length of sexual abstinence on sperm parameters and seminal plasma biochemical indicator.Methods Sperm concentration and motility were detected by computer assisted sperm analysis(CASA).The sperm morphology was analyzed using semiautomatic sperm morphology analyzer.Seminal plasma fructose concentration,neutral a-glycosidase,zinc,and acid phosphates concentration were measured by spectrophotometer.Improved DTNB was conducted for detection of the content of seminal plasma novain.Prostatespecific antigen(PSA)was measured with commercial kit.Three groups were defined according to the days of sexual abstinence:G1(1-3 days),G2(4-5 days)and G3(>6 days).Results Sperm concentration of G2 was significantly higher than that of G1(P<0.01),and of G3 than that of G1 and G2(P<0.01).Sperm motility and per centage of morphologically normal sperm of G3 were significantly lower than that of G1(P<0.01).Seminal plasma fructose concentration of G3 was significantly lower than that of G1 and G2(P<0.01,P<0.05).Seminal plasma neutral a-glycosidase,carnutine,zinc concentration of G3 was significantly higher than that of G1 and G2(P<0.01,P<0.05).Conclusion The length of sexual abstinence can influence sperm parameters and seminal plasma biochemical indicator.%目的 探讨禁欲时间对精子参数及精浆生化指标的影响.方法 采用精子形态检测系统下人工修正方法进行精子形态分析.采用精子质量检测系统进行精子密度、活力分析.精浆果糖含量、中性a糖苷酶、精浆锌、酸性磷酸酶等采用分光光度比色法测定.采用DTNB改进法检测精浆肉毒碱含量.前列腺特异性抗原(PSA)采用试剂盒进行检测.根据禁欲时间分为3组:G1(禁欲1~3d)组、G2(禁欲4~5d)组和G3(禁欲6d以上)组.结果G2组精子密度[(70.64±63.79)×106]显著高于G1组[(57.40±45.36)×106,P<0.01],G3组精子密度[(77.00±65.43)×106]显著高于G1组[(57.40±45.36)×106

  12. Effects of heme iron on growth performance and blood biochemical parameters of weaned piglets%血红素铁对断奶仔猪生长性能和血液指标的影响

    Institute of Scientific and Technical Information of China (English)

    高冬余; 王留香; 于伟; 江国永; 潘勇; 成国祥

    2012-01-01

    One hundred and twenty 28-day-old weaned piglets were selected and randomly divided into 4 groups, i.e. control, treatment 1, treatment 2 and treatment 3, which were respectively supplied (K27.5, 55.0 and 85.0 mg/kg heme iron on the basal diet. The effects of heme iron on growth performance and blood biochemical parameters (hemoglobin and IgG) of weaned piglets were researched. The feeding experiment was conducted for 13 days. The results as follows: heme iron could improve weaned piglet's average daily gain and feed conversion rate, Statistical analysis showed 55.0 mg/kg heme iron in diet increased feed conversion rate by 10.27% compared with control group (P<0.05). Heme iron had a certain growth promoting effect on weaned piglets, and the hemoglobin concentration in the whole blood and the IgG concentration in the plasma were improved.%选取120头28日龄断奶仔猪,随机分成4个处理组,即1个对照组和3个试验组,每组3个重复,每个重复10头.对照组饲喂基础日粮,试验1、2、3组分别饲喂基础日粮中血红素铁净添加量为27.5、55.0、85.0 mg/kg的3种试验日粮,研究日粮中添加血红素铁对断奶仔猪的生长性能、血液指标(血红蛋白浓度、免疫球蛋白浓度)的影响.试验正试期13d.结果表明,血红素铁各添加量组均能够提高仔猪的生长性能,日粮中血红素铁添加量为55 mg/kg时,仔猪平均日增重、饲料转化率较对照组分别提高12.02%和10.27%(P<0.05).血红素铁对断奶仔猪有良好的促生长作用,也在一定程度上提高了仔猪血液中血红蛋白和IgG浓度.

  13. Effect of Porosity and Concentration Polarization on Electrolyte Diffusive Transport Parameters through Ceramic Membranes with Similar Nanopore Size

    Directory of Open Access Journals (Sweden)

    Virginia Romero

    2014-08-01

    Full Text Available Diffusive transport through nanoporous alumina membranes (NPAMs produced by the two-step anodization method, with similar pore size but different porosity, is studied by analyzing membrane potential measured with NaCl solutions at different concentrations. Donnan exclusion of co-ions at the solution/membrane interface seem to exert a certain control on the diffusive transport of ions through NPAMs with low porosity, which might be reduced by coating the membrane surface with appropriated materials, as it is the case of SiO2. Our results also show the effect of concentration polarization at the membrane surface on ionic transport numbers (or diffusion coefficients for low-porosity and high electrolyte affinity membranes, which could mask values of those characteristic electrochemical parameters.

  14. In-vivo study for anti-hyperglycemic potential of aqueous extract of Basil seeds (Ocimum basilicum Linn) and its influence on biochemical parameters, serum electrolytes and haematological indices.

    Science.gov (United States)

    Chaudhary, Sachin; Semwal, Amit; Kumar, Hitesh; Verma, Harish Chandra; Kumar, Amit

    2016-12-01

    The study introduced anti-hyperglycemic influence of aqueous extract of Ocimum basilicum seeds (AEOBS) in Streptozotocin (STZ) induced diabetic rats and estimating its potential to ameliorate altered level of biochemical parameters, serum electrolytes level and haematological indices along with its effect on body weight of treated rats. The albino rats were selected to observe oral glucose tolerance test by oral intake of aq. glucose solution (4g/kg, body weight) in normal rats and estimation of blood glucose level after administration of AEOBS at 250mg/kg, 500mg/kg and standard drug glibenclamide at 0.6mg/kg, body weight. Antidiabetic activity was evaluated in chronic study models by STZ induced diabetes in rats followed by blood glucose estimation. Chronic study model was selected to carry out further studies to evaluate the effect of AEOBS at 250mg/kg, 500mg/kg and standard drug on body weight, alterations in biochemical parameters including AST, ALT, ALP, total bilirubin and total protein, alterations in serum electrolytes like Na(+), K(+), Cl(-), HCO3(-) along with estimation of haematological indices like red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), lymphocytes, neutrophils, eosinophils, monocytes and basophils. AEOBS significantly reduced the blood glucose level of diabetic rats at both doses. Body weight was also improved significantly. Similarly, the levels of biochemical parameters, serum electrolytes, and haematological indices were significantly ameliorated at both doses of AEOBS. The histopathological results revealed reconstitution of pancreatic islets towards normal cellular architecture in rats treated with AEOBS. The results illustrated that AEOBS have eminent antidiabetic potential in STZ effectuated diabetes in rats and can be extensively used for the treatment of diabetes mellitus-II and its associated complications including anaemia, diabetic nephropathy, liver dysfunction, and immunosuppression.

  15. Phase separation of polymer solutions. The calculation of the cloudpoint curve with a concentration and temperature-dependent free energy correction parameter

    NARCIS (Netherlands)

    Emmerik, van P.T.; Smolders, C.A.

    1973-01-01

    The free enthalpy correction parameter g in the Flory-Huggins equation for the Gibbs free enthalpy of mixing in polymer solutions is considered generally as a concentration- and temperature-independent parameter. It has been extended here with linear concentration- and temperature-dependent terms. W

  16. Blind Deconvolution for Distributed Parameter Systems with Unbounded Input and Output and Determining Blood Alcohol Concentration from Transdermal Biosensor Data.

    Science.gov (United States)

    Rosen, I G; Luczak, Susan E; Weiss, Jordan

    2014-03-15

    We develop a blind deconvolution scheme for input-output systems described by distributed parameter systems with boundary input and output. An abstract functional analytic theory based on results for the linear quadratic control of infinite dimensional systems with unbounded input and output operators is presented. The blind deconvolution problem is then reformulated as a series of constrained linear and nonlinear optimization problems involving infinite dimensional dynamical systems. A finite dimensional approximation and convergence theory is developed. The theory is applied to the problem of estimating blood or breath alcohol concentration (respectively, BAC or BrAC) from biosensor-measured transdermal alcohol concentration (TAC) in the field. A distributed parameter model with boundary input and output is proposed for the transdermal transport of ethanol from the blood through the skin to the sensor. The problem of estimating BAC or BrAC from the TAC data is formulated as a blind deconvolution problem. A scheme to identify distinct drinking episodes in TAC data based on a Hodrick Prescott filter is discussed. Numerical results involving actual patient data are presented.

  17. Achieving concentrated graphene dispersions in water/acetone mixtures by the strategy of tailoring Hansen solubility parameters

    Science.gov (United States)

    Yi, Min; Shen, Zhigang; Zhang, Xiaojing; Ma, Shulin

    2013-01-01

    Although exfoliating graphite to give graphene paves a new way for graphene preparation, a general strategy of low-boiling-point solvents and high graphene concentration is still highly required. In this study, using the strategy of tailoring Hansen solubility parameters (HSP), a method based on exfoliation of graphite in water/acetone mixtures is demonstrated to achieve concentrated graphene dispersions. It is found that in the scope of blending two mediocre solvents, tailoring the HSP of water/acetone mixtures to approach the HSP of graphene could yield graphene dispersions at a high concentration of up to 0.21 mg ml-1. The experimentally determined optimum composition of the mixtures occurs at an acetone mass fraction of ˜75%. The trend of concentration varying with mixture compositions could be well predicated by the model, which relates the concentration to the mixing enthalpy within the scope of HSP theory. The resultant dispersion is highly stabilized. Atomic force microscopic statistical analysis shows that up to ˜50% of the prepared nanosheets are less than 1 nm thick after 4 h sonication and 114g centrifugation. Analyses based on diverse characterizations indicate the graphene sheets to be largely free of basal plane defects and oxidation. The filtered films are also investigated in terms of their electrical and optical properties to show reasonable conductivity and transparency. The strategy of tailoring HSP, which can be easily extended to various solvent systems, and water/acetone mixtures here, extends the scope for large-scale production of graphene in low-boiling-point solutions.

  18. Uncertainty of Blood Alcohol Concentration (BAC Results as Related to Instrumental Conditions: Optimization and Robustness of BAC Analysis Headspace Parameters

    Directory of Open Access Journals (Sweden)

    Haleigh A. Boswell

    2015-12-01

    Full Text Available Analysis of blood alcohol concentration is a routine analysis performed in many forensic laboratories. This analysis commonly utilizes static headspace sampling, followed by gas chromatography combined with flame ionization detection (GC-FID. Studies have shown several “optimal” methods for instrumental operating conditions, which are intended to yield accurate and precise data. Given that different instruments, sampling methods, application specific columns and parameters are often utilized, it is much less common to find information on the robustness of these reported conditions. A major problem can arise when these “optimal” conditions may not also be robust, thus producing data with higher than desired uncertainty or potentially inaccurate results. The goal of this research was to incorporate the principles of quality by design (QBD in the adjustment and determination of BAC (blood alcohol concentration instrumental headspace parameters, thereby ensuring that minor instrumental variations, which occur as a matter of normal work, do not appreciably affect the final results of this analysis. This study discusses both the QBD principles as well as the results of the experiments, which allow for determination of more favorable instrumental headspace conditions. Additionally, method detection limits will also be reported in order to determine a reporting threshold and the degree of uncertainty at the common threshold value of 0.08 g/dL. Furthermore, the comparison of two internal standards, n-propanol and t-butanol, will be investigated. The study showed that an altered parameter of 85 °C headspace oven temperature and 15 psi headspace vial pressurization produces the lowest percent relative standard deviation of 1.3% when t-butanol is implemented as an internal standard, at least for one very common platform. The study also showed that an altered parameter of 100 °C headspace oven temperature and 15-psi headspace vial pressurization

  19. [Biochemical parameters of blood and morpho-functional state of the liver of experimental animals by the actions of lead sulfide nanoparticles in different time study].

    Science.gov (United States)

    Omel'chuk, S T; Aleksiĭchuk, V D; Sokurenko, L M

    2014-01-01

    Biochemical studies revealed that alanine aminotransferase levels changing first during short action (30 injections) of lead sulfide nanoparticles of size 10 and 30 nm, and the ionic form of a 400 nm lead while the growth of both enzymes (aspartate aminotransferase and alanine aminotransferase) activity during long-term exposure (60 injections) is the same intensity. It it confirmed by the value of de Ritis coefficient, which is statistically the same as control. Morphological studies also confirm these data--degenerative changes of hepatocytes, reactive changes of the stroma and vascular responses were detected. It is shown that the severity of metabolic and morphological damages in the liver increased with prolonging the duration of lead nanoparticles intake.

  20. Effect of calcium soap of fatty acids supplementation on serum biochemical parameters and ovarian activity during out-of-the-breeding season in crossbred ewes.

    Science.gov (United States)

    El-Nour, Hayat H M; Nasr, Soad M; Hassan, Walid R

    2012-01-01

    This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA) supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45-55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (P breeding season.

  1. THE IN-VIVO EFFECTS OF PARTIALLY PURIFIED SUB-FRACTION (TAF2 OF THE CRUDE METHANOLIC EXTRACT OF EURYCOMA LONGIFOLIA ROOTS ON THE HAEMATOLOGICAL, BIOCHEMICAL AND HISTOLOGY PARAMETERS

    Directory of Open Access Journals (Sweden)

    Narazah Mohd Yusoff et al

    2012-09-01

    Full Text Available Eurycoma longifolia (E. longifolia Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial, anti-pyretic and anticancer activities. As yet, no study has been done on biosafety of partially purified sub-fraction (TAF2 of the crude methanolic extract of E. longifolia roots. This study aims to evaluate, in vivo, the effects of TAF2 on the haematological, biochemical and histology parameters in nude mice. Male nude mice were treated orally with TAF2 (200 mg/Kg/mouse every other day for 16 days. Untreated mice were used as controls. Blood samples were collected by intra-cardiac puncture and haematological and biochemical parameters were analyzed by using animal blood counter and automated chemistry analyzer, respectively. Hematoxylin-eosin stain (H&E was used to evaluate the histopathology changes. Blood cells morphology was assessed by Wright stain of the thin blood films. The results showed normal blood haematology and clinical chemistry. Histological examination of TAF2 treated vital organs (kidney, liver and spleen of mice showed no apparent change with the dose used. In conclusion, E. longifolia root methanolic extract is safe on short-term exposur.

  2. Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

    Directory of Open Access Journals (Sweden)

    Nilton Massuo Ishikawa

    2007-07-01

    Full Text Available Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758 was investigated by the hematological parameters after long-term (14 days exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg. Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC, hemoglobin concentration (Hb, hematocrit (Ht, mean corpuscular volume (MCV, mean corpuscular hemoglobin (MCH, mean corpuscular hemoglobin concentration (MCHC, total white blood cell count (WBC and differential leukocyte counts and total thrombocyte count (Tr. There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.A toxicidade do mercúrio foi avaliada em tilápia, Oreochromis niloticus (Linnaues, 1758 através da análise dos parâmetros hematológicos após exposição a diferentes concentrações sub-letais, durante um período de 14 dias. O bioensaio foi conduzido no laboratório de toxicologia do Instituto de Pesca, SP. Foram utilizados alevinos (12.44 ± 0.84 cm, e 27.13 ± 4.67 g e aquários com capacidade para 50 litros e preenchidos com água declorada e mais a quantidade de solução de mercúrio (HgCl2 correspondendo as seguintes concentrações: 0,02; 0,002; 0.0002 mg.L-1 Hg. Foram utilizadas 3 repetições de cada concentração e grupo controle. Amostras de sangue foram coletadas de seis animais de cada concentração nos tempos 0, 3, 7, 10 e 14 dias de exposição. Foram avaliados: a contagem de eritrócitos (RBC, concentração de hemoglobina (Hb, hematócrito (Ht, volume corpuscular médio (VCM, hemoglobina corpuscular média (HCM e concentração de hemoglobina corpuscular média (CHCM, trombócitos totais (Tr, contagem

  3. Effects of buffering mineral mixtures on milk yield, milk composition, rumen pH and some blood biochemical parameters in heat stressed dairy cows

    Directory of Open Access Journals (Sweden)

    Adamović Milan

    2014-01-01

    Full Text Available The objective of the work was to investigate the influence of partial substitution of magnesium oxide with natural bentonite in feed mixtures used in feeding of cows during their exposure to heat stress. The investigation lasted 30 days and was carried out during last ten days of may and first twenty days of June when average air temperature in stables was 36.6±2.5oC. In the experiment there were included 30 cows of Holstein breed in first phase of lactation, which were divided into two groups of 15 cows: control (C and experimental (E. Group C was fed with experimental mineral mixture that contained 60% of magnesium oxide during the whole investigation period. Group E was fed with experimental mineral mixture that contained 40% of magnesium oxide as well as 20% of natural bentonite. Remaining ingredients in both control and experimental mineral mixtures were the same and also contained 20% of sodium bicarbonate and 20% of zeolite in the same quantities. The control and experimental mineral mixtures were mixed into complete feed mixture (18% UP in the amount of 1%. At tne end of the investigation period, on the 30th day, there were taken samples of rumens contents for determining pH, and after that blood samples, in which, after the separation of blood serum, were determined glucose concentration, total proteins, albumin, globulin, urea, HDL cholesterol, LDL cholesterol, total bilirubin, calcium and phosphorus, as well as the activity of ALT and AST. By computation there was calculated the ratio between albumin and globulin, ALT and AST, and the ratio between calcium and phosphorus. Daily allowance and milk chemical composition ( percentage of fat, proteins and dry substance were determined at the end of the investigation period, that is on the 30th day of lactation, for each cow individually. Partial substitution of magnesium oxide with bentonite influenced milk production increase, but it was statistically insignificant. Besides that, in E

  4. Feasibility and Operation of High Concentration Waste Water Impact of UNITANK Process Biochemical System%UNITANK工艺应对高浓度废水冲击操作的可行性研究

    Institute of Scientific and Technical Information of China (English)

    杨明远; 董国华; 汪巧雯

    2016-01-01

    Feed with high concentration and degration-resistant organic mater, produce enormous impact for the biochemical operation system which has acclimatized municipal sewage, have a strong impact on treatment effect of biochemical system, the work statement of biochemical flora, the effluent quality, the COD emission reductions and, the normal production operation, the biochemical system seems slowly to recover after impact and, to last a long time. With a view to the treatment process traits of waste water treatment plant, analyzed the effects that resulting from high concentration and degration-resistant organic waste wa-ter impact, and based on the theory of waste water treatment and, the test of leachate and municipal sewage synergy treatment and, the impact test of different COD charge, and the key technology of high concentration and degration-resistant waste water treatment. Analyzed the feasibility operation, and put forward reference projects. In the process of daily operation management in waste water treatment plant, with a view to solutions to adjust and operate production, ensure stable operation statement and stable sludge concentration and activity, avoid the accumulate of biotoxin in biological system. Standard operation management, ensure biological system operate in suitable work conditions, avoid resulting from feed water quality and improper operation cause the change of environment of microbes growing and the structure of biotic population, resulting from abnormal problems happen. Thus at the accident statements, positively respond to ensure the treatment and operation effect, and COD emission reduction quantity, and stable operation system.%高浓度难降解有机废水对已经适应了城市污水处理的生化处理系统造成了巨大的冲击,严重影响了生化系统的处理效果,以及正常的生产操作,同时生化系统受冲击后的恢复显得异常缓慢、延续的时间也较长.针对生产的操作,论述了应对措施

  5. Meteorological parameters contributing to variability in 222Rn activity concentrations in soil gas at a site in Sapporo, Japan.

    Science.gov (United States)

    Fujiyoshi, Ryoko; Sakamoto, Keita; Imanishi, Tsukushi; Sumiyoshi, Takashi; Sawamura, Sadashi; Vaupotic, Janja; Kobal, Ivan

    2006-10-15

    Continuous (222)Rn monitoring in soil gas since November 22, 2004 has revealed variability in activity concentration with time in the semi-natural woods on the campus of Hokkaido University in Sapporo, Japan. Among various factors affecting soil radon levels and variability, temperature was found to be dominant during three seasons when activity concentrations of (222)Rn showed a diurnal high and nocturnal low with a boundary around 10 o'clock in the morning. This pattern was disturbed by low pressure fronts with occasional rain. The activity gradually decreased as soil temperatures decreased from late November to mid-December. After the ground surface was completely covered with snow, soil radon levels became low with a small fluctuation. There were several peaks of (222)Rn on the time-series chart in winter. Those peaks appearing in early winter and early spring may be interpreted by considering meteorological parameters. In a few cases, the radon activity suddenly increased with increasing pressure in the soil at a depth of 10 cm, which may be associated with subsurface events such as seismic activity in the area.

  6. Effect of Milk Thistle (Silybum marianum L. on Biochemical Parameters and Immunity of Broiler Chicks Fed Aflatoxin B1 after Three Weeks

    Directory of Open Access Journals (Sweden)

    Maliheh Amiri Dumari

    2014-09-01

    Full Text Available Background: This study was conducted to determine the efficacy of milk thistle seeds (MTSs in counteracting the toxic effects of aflatoxin B1 (AFB1 in a contaminated diet fed to broilers. Methods: Two dietary inclusion rates of AFB1 (0, 0.250 and 500 ppb and MTS (0, 0.5 and 1% were tested in a 3×3 factorial manner. The effect of nine experimental treatments was assessed using 216 one-d-old Ross 308 male broiler chicks in a randomized complete design with 4 replicates of 6 birds each from one to 21 days of age. The effects of dietary AFB1 and MTS on serum biochemistry factors, antibody titer against Newcastle disease (ND and influenza disease (ID in broilers were evaluated at the end of this period. Results: Statistical analysis of the main effects of diets indicated no significant changes in uric acid, cholesterol, triglycerides, low density lipoprotein (LDL, ID, and phosphorus compared to the control (P>0.01. Also, addition of 500 ppb of dietary AFB1 into the diet was associated with significant decreases in serum glucose, calcium, high density lipoprotein (HDL, and ND compared to the control group (P<0.01. The contaminated diet significantly increased the activities of aspartate aminotransferase (AST and alanine aminotransferase (ALT (P<0.05. Conclusion: Milk thistle showed protective effects and resulted in some serum enzyme activities and serum biochemical changes associated with aflatoxin toxicity.

  7. Effect of Calcium Soap of Fatty Acids Supplementation on Serum Biochemical Parameters and Ovarian Activity during Out-of-the-Breeding Season in Crossbred Ewes

    Directory of Open Access Journals (Sweden)

    Hayat H. M. El-Nour

    2012-01-01

    Full Text Available This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45–55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (<0.05 increases in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and progesterone levels with decrease in calcium and phosphorous levels in treated group. In treated group, normal-size ovaries and more than one follicle on the ovaries were detected and pregnancy rate increased. In conclusion, CSFA supplementation was effective to maintain the reproductive performance when ewes were out of the breeding season.

  8. Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats.

    Science.gov (United States)

    Amin, K A; Abdel Hameid, H; Abd Elsttar, A H

    2010-10-01

    Tartrazine and carmoisine are an organic azo dyes widely used in food products, drugs and cosmetics. The present study conducted to evaluate the toxic effect of these coloring food additives; on renal, hepatic function, lipid profile, blood glucose, body-weight gain and biomarkers of oxidative stress in tissue. Tartrazine and carmoisine were administered orally in two doses, one low and the other high dose for 30 days followed by serum and tissue sample collection for determination of ALT, AST, ALP, urea, creatinine, total protein, albumin, lipid profile, fasting blood glucose in serum and estimation of GSH, catalase, SOD and MDA in liver tissue in male albino rat. Our data showed a significant increase in ALT, AST, ALP, urea, creatinine total protein and albumin in serum of rats dosed with tartrazine and carmoisine compared to control rats and these significant change were more apparent in high doses than low, GSH, SOD and Catalase were decreased and MDA increased in tissue homogenate in rats consumed high tartrazine and both doses of carmoisine. We concluded that tartrazine and carmoisine affect adversely and alter biochemical markers in vital organs e.g. liver and kidney not only at higher doses but also at low doses.

  9. Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats.

    Science.gov (United States)

    Ragy, Merhan Mamdouh

    2015-01-01

    Increasing use of mobile phones in daily life with increasing adverse effects of electromagnetic radiation (EMR), emitted from mobile on some physiological processes, cause many concerns about their effects on human health. Therefore, this work was designed to study the effects of exposure to mobile phone emits 900-MHz EMR on the brain, liver and kidney of male albino rats. Thirty male adult rats were randomly divided into four groups (10 each) as follows: control group (rats without exposure to EMR), exposure group (exposed to 900-MHz EMR for 1 h/d for 60 d) and withdrawal group (exposed to 900-MHz electromagnetic wave for 1 h/d for 60 d then left for 30 d without exposure). EMR emitted from mobile phone led to a significant increase in malondialdehyde (MDA) levels and significant decrease total antioxidant capacity (TAC) levels in brain, liver and kidneys tissues. The sera activity of alanine transaminase (ALT), aspartate aminotransferase (AST), urea, creatinine and corticosterone were significantly increased (p electromagnetic field emitting from mobile phone might produce impairments in some biochemicals changes and oxidative stress in brain, liver and renal tissue of albino rats. These alterations were corrected by withdrawal.

  10. Hepato Protective Efficacy of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and Their Formulation on Imidacloprid Induced Liver Toxicity by Histopathological and Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    V. Eugin Amala

    2015-09-01

    Full Text Available Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations are used as a phytotherapeutic medicine for hepatic diseases. In the present study the effect of methanolic extract of herbal powder was administered against Imidacloprid in Albino Wistar Rats. Single dose of herbal (500 mg/kg/b.w was given orally for 28 days to Imidacloprid toxicity exposed to rats. They were observed each hour and each day for 28 days for any changes in behavioural activity loss of ability to move, cramping, lethargy, muscle weakness, convulsion, irritation of the eye, excess salivation, change in gait. Clinical observations body weight, organ weight, biochemical analysis and histopathological examination were carried out. Administration of Imidacloprid, produced liver degeneration such as cytoplasmic vacuolation, mild vacuolar degeneration, mild hepatic damage in low dose (40 mg/kg b.w and appearance of blood streaks, hepatic damage and severe vacuolar degenerations were observed in high dose of induction (80 mg/kg b.w. Animals were treated with the phytotherapeutic products of Terminalia chebula, Terminalia bellirica, Phyllanthus emblica and their formulations. Triphala extract was possessed more hepatoprotective activity, while comparing the four samples which was proved experimentally.

  11. Concentrations of total glutathione and cysteine in wheat flour as affected by sulfur deficiency and correlation to quality parameters.

    Science.gov (United States)

    Reinbold, Julia; Rychlik, Michael; Asam, Stefan; Wieser, Herbert; Koehler, Peter

    2008-08-27

    A method for the simultaneous quantitation of total glutathione and total cysteine in wheat flour by a stable isotope dilution assay using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) was developed. As internal standards, L-[(13)C3, (15)N]cysteine and L-gamma-glutamyl-L-[(13)C3, (15)N]cysteinyl-glycine were used. The method consisted of the extraction and reduction of flour with tris(2-carboxyethyl) phosphine after the addition of internal standards, protection of free thiol groups with iodoacetic acid, derivatization of free amino groups with dansyl chloride, and HPLC-MS/MS. The limits of detection and quantitation for glutathione were 0.75 nmol/g and 2.23 nmol/g flour, respectively. For cysteine, the limits of detection and quantitation were 0.72 nmol/g and 2.12 nmol/g flour, respectively. The developed method was found to be sensitive enough for quantitation of total glutathione and cysteine levels in wheat flour. This method was then utilized to investigate the effect of sulfur (S) deficiency on the amount of total glutathione and cysteine in flour. In S-deficient wheat, the concentrations of total glutathione and cysteine were proportional to the amount of S supplied during growth. The calculation of correlations revealed that GSH and Cys concentrations influenced the rheological dough properties and the baking performance at least as much as protein parameters. Thus, the low concentration of GSH and Cys in flour from S-deficient wheat had a similar effect on the technological properties as the altered composition of gluten proteins.

  12. Leaf photosynthesis and respiration of three bioenergy crops in relation to temperature and leaf nitrogen: how conserved are biochemical model parameters among crop species?

    NARCIS (Netherlands)

    Archontoulis, S.V.; Yin, X.; Vos, J.; Danalatos, N.G.; Struik, P.C.

    2012-01-01

    Given the need for parallel increases in food and energy production from crops in the context of global change, crop simulation models and data sets to feed these models with photosynthesis and respiration parameters are increasingly important. This study provides information on photosynthesis and r

  13. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose pgender between women and men serum levels, ALT and AST levels; p<0.006 and cholesterol; p<0.04 were detected. According to participants education level blood biochemistry levels were observed statisticaly different p<0.001 with non-literacy group. In conclusion, obese and obese+diabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.

  14. Evaluation of biological and biochemical quality of whey protein.

    Science.gov (United States)

    Haraguchi, Fabiano Kenji; Pedrosa, Maria Lucia; Paula, Heberth de; Santos, Rinaldo Cardoso dos; Silva, Marcelo Eustáquio

    2010-12-01

    Nutritional and biochemical properties of noncommercial whey protein have been described since 1950. However, comparisons between commercial whey protein for human consumption and casein are rarely found. The aim of this study was to compare biological quality of a commercial whey protein with casein and its effect on biochemical parameters of rats. Thirty-two weanling Fisher rats were divided into three groups and given the following diets: casein group, standard diet (AOAC); whey protein group, modified AOAC diet with whey protein instead of casein; and casein:whey group, modified AOAC diet with 70%:30% casein:whey. A protein-free group was used for determination of endogenous nitrogen losses. Net protein ratio, protein efficiency ratio, and true digestibility were determined, and blood was collected for biochemical analysis. When compared with casein, whey protein showed significant differences for all biological parameters evaluated, as well as for albumin, total protein, total cholesterol, and glucose concentrations. Replacing 30% of casein with whey protein did not affect these parameters. A positive relation among whey protein, high-density lipoprotein-cholesterol, and paraoxonase activity was found. Hepatic or renal dysfunctions were not observed. In conclusion, in comparison with casein, commercial whey protein had higher values of biological parameters, and biochemical evaluation revealed it improved glycemic homeostasis, lipid status, and paraoxonase activity in rats.

  15. Effects of exposure to three environmental chemicals on the selected biochemical parameters of the blood plasma of rainbow trout, Oncorhynchus mykiss (Walbaum

    Directory of Open Access Journals (Sweden)

    Řehulka Jiří

    2016-03-01

    Full Text Available Rainbow trout Oncorhynchus mykiss at a weight of 115 ± 24 g (mean ± SD were experimentally injected with di(2-ethylhexylphthalate (DEHP, 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD and gamma isomer of hexachlorocyclohexane (γ-HCH to evaluate nitrogen metabolism (total protein [TP], blood urea nitrogen, uric acid [UA], creatinine, carbohydrate metabolism (glucose, mineral metabolism (inorganic phosphate[P], total calcium [Cat], and catalytic activity of enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], alkaline phosphatase [ALP], lactate dehydrogenase [LD]. After 21 days, the tested xenobiotics, administered by the intraperitoneally route, caused the following effect: in comparison with negative controls, fish injected with DEHP at concentrations of 200 or 50 mg kg-1 of body weight were found to have a higher level of P and lower level of Cat and decreased catalytic concentrations of ALT, AST, ALP and LD in the plasma of the peripheral blood. A higher level of TP and P and a decreased catalytic concentration of ALT were found when TCDD was administered at a dose of 2 μg kg-1. A decrease in the catalytic concentrations of ALT and ALP occurred in fish injected with γ-HCH in a dose of 50 mg kg-1. UA and P levels were decreased in fish injected with γ-HCH in a dose of 5 mg kg-1. DEHP and TCDD caused neutrophilic leucocytosis with a marked left shift. Both concentrations of γ-HCH led to an increase in polychromatophilic erythrocytes. The frequency of micronuclei varied in all experimental groups, including the controls, ranging between 0.8 and 2.1 ‰. Histological examination in fish injected with DEHP at a concentration of 200 mg kg-1 revealed eosinophilic droplets in the epithelium of renal tubules and histological examination in fish injected with γ-HCH at a higher concentration revealed an increased activation of sinusoidal cells in the liver, a fibrous thickening of bile duct walls, bile duct hyperplasia and a

  16. Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus fingerlings

    Directory of Open Access Journals (Sweden)

    Shao Wei Zhai

    2014-07-01

    Full Text Available The present study was performed with tilapia (Oreochromis niloticus to evaluate the effects of diet supplementation with grape seed proanthocyanidins (GSPs on fish growth performance, some serum parameters and body composition. Three hundred tilapia fingerlings with the initial average body weight of 9.50±1.25 g were randomly divided into five treatment groups with four replicates in each group and 15 fish in each replicate. The dietary GSPs levels of five treatment groups were 0 (control group, 200, 400, 600, and 800 mg/kg, respectively. The trial period was 49 days. Growth performance parameters were significantly improved by GSPs supplementation (P<0.05, while survival rates were similar among all groups (P>0.05. Serum parameter results showed that activities of aminotransferase aspartate in 200 and 400 mg/kg GSPs groups and alanine aminotransferase in 400 mg/kg GSPs group were lowered significantly (P<0.05. Levels of triglyceride and total cholesterol (except 200 mg/kg GSPs group were significantly lowered, while lysozyme activity and albumin level were significantly higher in fish of GSPs supplemented groups, independently from the level of supplementation. The highest crude protein level and lowest crude lipid level were found in fish of all GSPs supplemented groups, while levels of moisture and ash in fish of all groups were similar (P>0.05. The results indicated that dietary 200 mg/kg GSPs could exert beneficial effects on growth and body composition of tilapia fingerlings, and ameliorate serum biochemistry parameters related to health status.

  17. Studies on intermolecular interaction on binary mixtures of methyl orange-water system: excess molar functions of ultrasonic parameters at different concentrations and at different temperatures.

    Science.gov (United States)

    Thanuja, B; Kanagam, Charles; Sreedevi, S

    2011-11-01

    Density (ρ), viscosity (η) and ultrasonic velocity (u) of binary mixtures of methyl orange and water were measured at different concentrations and at different temperatures; several useful parameters such as excess volume, excess velocity, and excess adiabatic compressibility have been calculated. These parameters are used to explain the nature of intermolecular interactions taking place in the binary mixture. The above study is helpful in understanding the dye/solvent interaction at different concentration and temperatures.

  18. Hematological, biochemical, and behavioral responses of Oncorhynchus mykiss to dimethoate.

    Science.gov (United States)

    Dogan, Demet; Can, Canan

    2011-12-01

    The effects of dimethoate on hematological, biochemical parameters, and behavior were investigated in Oncorhynchus mykiss exposed to sublethal concentrations of 0.0735, 0.3675, and 0.7350 mg/l for 5, 15, and 30 days. Significant decrease was determined in erythrocyte and leukocyte counts, hemoglobin, hematocrit, MCV, and MCH, which was pronounced after prolonged exposure indicating the appearance of microcytic hypochromic anemia. There were no prominent changes in thrombocyte and MCHC. The glucose concentration showed an ascending pattern that proved to be positively correlated with duration. The protein concentration declined in higher dimethoate concentrations following 15 and 30 days. Negative and significant correlation was detected between glucose and protein concentrations. The fish showed remarkable behavioral abnormality such as loss of balance, erratic swimming, and convulsion. Present findings revealed that dimethoate exerts its toxic action even in sublethal concentrations and hematological parameters and abnormal behavior may be sensitive indicators to evaluate pesticide intoxication.

  19. 甲状腺功能亢进的糖尿病患者生化指标变化%Biochemical parameters change in diabetic patients with hyperthyroidism

    Institute of Scientific and Technical Information of China (English)

    方欣臣; 叶书来

    2014-01-01

    Objective To observe the biochemical markers of type 2 diabetes mellitus(T2DM)patients with hyperthyroidism,to investigate the effects of hyperthyroidism in patients with T2DM.Methods Retrospective analysis was performed in blood sugar,blood lipid,renal function and other indicators of 72 T2DM cases with thyroid function abnormality(hyperthyroidism group)and 140 T2DM with normal thyroid function (control group).Results Compared with the control group,triglyceride,total cholesterol,low density lipoprotein cholesterol,glycated hemoglobin, creatinine in hyperthyroidism group decreased, while there were no significant difference in fasting glucose, postprandial blood glucose two hours,high density lipoprotein cholesterol,very low density lipoprotein and urinary albumin/creatinine between two groups.Conclusion Although individual indicators in T2DM cases with thyroid function abnormality decreased,the patients share similar risk in glucose metabolism disorders,kidney damage, vascular complications,and attention should be paid on the treatment.%目的:观察合并甲状腺功能亢进(甲亢)的2型糖尿病(type 2 diabetes mellitus,T2DM)患者的生化指标变化,探讨甲亢对 T2DM 患者的影响。方法回顾性分析72例 T2DM 合并甲状腺功能异常的患者(甲亢组)与140例 T2DM 甲状腺功能正常患者(对照组)的血糖、血脂、肾功能等变化。结果与对照组比较,甲亢组甘油三脂、总胆固醇、低密度脂蛋白胆固醇、糖化血红蛋白、肌酐降低,而两组空腹血糖、餐后两小时血糖、高密度脂蛋白胆固醇、极低密度脂蛋白及尿白蛋白/肌酐、肾小球滤过率差异均无统计学意义。结论伴有甲亢的 T2DM 患者虽然个别指标降低,但在糖代谢紊乱、肾脏损伤、大血管并发症方面有着同样的风险,治疗上应予以重视。

  20. Modelling the anaerobic digestion of solid organic waste - Substrate characterisation method for ADM1 using a combined biochemical and kinetic parameter estimation approach.

    Science.gov (United States)

    Poggio, D; Walker, M; Nimmo, W; Ma, L; Pourkashanian, M

    2016-07-01

    This work proposes a novel and rigorous substrate characterisation methodology to be used with ADM1 to simulate the anaerobic digestion of solid organic waste. The proposed method uses data from both direct substrate analysis and the methane production from laboratory scale anaerobic digestion experiments and involves assessment of four substrate fractionation models. The models partition the organic matter into a mixture of particulate and soluble fractions with the decision on the most suitable model being made on quality of fit between experimental and simulated data and the uncertainty of the calibrated parameters. The method was tested using samples of domestic green and food waste and using experimental data from both short batch tests and longer semi-continuous trials. The results showed that in general an increased fractionation model complexity led to better fit but with increased uncertainty. When using batch test data the most suitable model for green waste included one particulate and one soluble fraction, whereas for food waste two particulate fractions were needed. With richer semi-continuous datasets, the parameter estimation resulted in less uncertainty therefore allowing the description of the substrate with a more complex model. The resulting substrate characterisations and fractionation models obtained from batch test data, for both waste samples, were used to validate the method using semi-continuous experimental data and showed good prediction of methane production, biogas composition, total and volatile solids, ammonia and alkalinity.

  1. Evolution of biochemical parameters in irradiated fishes: Serum proteins and intestinal nucleic acids; Evolucion de parametros bioquimicos en peces irradiados: Proteinas en suero y acidos nucleicos en intestino

    Energy Technology Data Exchange (ETDEWEB)

    Garces, F.; Andres, P.; Davila, C. A.

    1976-07-01

    In sublethal gamma-irradiated C. auratus, a sudden decrease of total serum protein concentration and a preferential descent of the low molecular weight gamma-globulin fraction have been observed. These effects are transient and after different latent periods dependent on doses, normal values are recovered, A temporal failure of a vascular permeability regulation system is probably implied. The DMA depolymerization. observed in the intestine indicates the action of radio-induced DNA degradation mechanisms since this effect is independent on doses. (Author) 29 refs.

  2. 黄芪建中汤对柔道选手血液及生化指标效应之研究%Effects of Huangqi Jianzhong Tang on hematological and biochemical parameters in judo athletes

    Institute of Scientific and Technical Information of China (English)

    苏奕彰; 林健蓉; 陈洸艟; 李铭; 林睿珊; 蔡真真; 周玉; 林昭庚

    2001-01-01

    目的:本研究在探讨黄芪建中汤对柔道选手血球及血清生化值的影响.方法:实验中以新民商工高中组柔道选手为对象,男生16名女生8名,采用分层分组的方法,分为实验组与对照组,分别服用黄芪建中汤与安慰剂.实验期间为五周,分前、后二次测量,检验选手血液中血球及血清生化值.结果以配对t检定、变异数分析法、单纯主要效果比较进行分析.结果:研究结果显示,血液中血球(RBC、Hb、Hct)指数于实验前后有显著降低,血清生化值(GOT、GPT、BUN、CK)于实验前后有显著升高,证实训练对血球及血清生化值有影响.结论:黄芪建中汤对柔道选手血球及血液生化值无显著效果,亦无不良之副作用.%AIM: The purpose of the study was to investigate the effects of Huangqi Jianzhong Tang (HQJZT) on hematological and biochemical parameters in judo athletes. METHODS: Sixteen male and eight female judo athletes in Hsin-Ming senior high school were randomly and stratified divided into control and experimental group, which received placebo and HQJZT respectively during the five-week training program. The measurement of the hematological and biochemical parameters was performed twice, just before and after the training. The data was analyzed with paired-t test and ANOVA. RESULTS: The values of RBC, Hb, and Hct were obvious decreased after intervention, while the value of GOT, GPT, BUN, and CK was elevated. CONCLUSION: The results indicated the hematological and biochemical changes were caused by the physical training but not the effects of HQJZT. The HQJZT had no adverse effects on the judo athletes in our study.

  3. Evaluation of growth, biochemical and bioaccumulation parameters in Pelophylax perezi tadpoles, following an in-situ acute exposure to three different effluent ponds from a uranium mine

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Sérgio M., E-mail: s.reis.marques@gmail.com [Departamento de Biologia da Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Chaves, Sandra [Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Edifício ICAT, Campus da FCUL Campo Grande, Lisboa (Portugal); Gonçalves, Fernando [Departamento de Biologia da Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Pereira, Ruth [CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Departamento de Biologia da Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto (Portugal)

    2013-02-15

    Mining activities invariably produce metal contaminated effluents. Depending on factors such as pH and metal concentration the toxicity of the effluent may vary. To assess the effects of three characteristically different effluent ponds from a deactivated uranium mine, with toxicologically relevant data, an in situ exposure with Pelophylax perezi tadpoles, was conducted. Tadpoles were exposed to the three effluent ponds, ranked by increasing order of metals concentrations (REF, M1, M2). Survival, growth, metal accumulation, antioxidant enzymes (catalase, glutathione peroxidase and glutathione reductase) and lipid peroxidation (LPO) were determined in tadpoles. As well, physical and chemical variables of the effluents were measured. Death percentage in the effluents was 3.17 (REF), 9.84 (M1) and 42.86% (M2) and was not coincident with metal accumulation which was highest in tadpoles exposed to M1, while metal contents in M2 tadpoles were quite similar to those recorded in REF tadpoles. However, high mortality in M2 was attributed to the extremely low pH (≈ 3.77). From the three effluents M2 tadpoles had the lowest growth and the antioxidant enzymatic activity was only affected in the case glutathione peroxidase (GPx) with significantly higher activity in M1, being in accordance with the highest accumulation of metals. LPO, usually associated with metal accumulation, had the following pattern M1 > REF > M2. Overall, effluent toxicity in tadpoles exposed to M2 effluent seems to be primarily an effect of pH while in M1 toxicity is mainly owed to high metal concentrations. The effluent acidity seems to reduce metal accumulation probably due to damage in the integument, affecting ion uptake. The results obtained bring a better understanding of the toxicological processes that local P. perezi population is subjected to, mainly in the early life stages. Furthermore this study highlights the influence of pH in the toxicity of metal rich effluents. - Highlights:

  4. Correlation between Semen PH Value and Semen Parameters or Biochemical Analyses Results of Seminal Plasma%精液pH值与精液参数及精浆生化的相关性研究

    Institute of Scientific and Technical Information of China (English)

    陈帅垒; 王祖龙

    2012-01-01

    目的 探讨精液pH值与精液参数及精浆生化之间的相关性,明确精液pH值在精液质量检测中所具有的价值.方法 收集河南省中医院中西医结合生殖中心2009年1月-2011年3月资料完整的男性不育症患者精液分析及精浆生化记录616份,建立Excel数据库,采用SPSS 16.0统计软件,对精液pH值与精液参数及精浆生化进行相关性分析.结果 精液pH值和受试者精浆弹性蛋白酶、精液A级精子百分率、前向运动精子百分率及存活精子百分率呈正相关性(P<0.05);与精浆酸性磷酸酶、精浆锌呈负相关性(P<0.05);与年龄、精浆果糖、精浆α-糖苷酶、精液白细胞、精液量、液化时间、精子浓度、正常形态精子数量无明显相关性(P>0.05).结论 精液pH值和精液参数及精浆生化的多项数值均具有相关性,进一步研究精液pH值的影响,对于男性不育症患者的诊疗具有重要意义.%Objective To study the correlation between semen pH value and semen parameters and biochemical analyses results of seminal plasma, and evaluate the value of semen pH in the semen quality test. Methods The semen and biochemical analyses results of 616 male infertility patients in our hospital from January 2009 to March 2011 were collected and analyzed by u-sing SPSS statistical software to evaluate the correlation between semen pH value and semen parameters and biochemical analyses results of seminal plasma. Results The semen PH value was positively correlated to the seminal plasma elastase, rate of grade A sperm,rate of rapid progression motility sperm and sperm viability (P 0. 05) . Conclusion The semen PH value is closely correlated to the semen parameters and biochemical analyses results of seminal plasma. The further study on the influence of semen PH value should improve the diagnosis and treatment of male infertility patients.

  5. Interactive effects of elevated CO2 concentration and irrigation on photosynthetic parameters and yield of maize in Northeast China.

    Directory of Open Access Journals (Sweden)

    Fanchao Meng

    Full Text Available Maize is one of the major cultivated crops of China, having a central role in ensuring the food security of the country. There has been a significant increase in studies of maize under interactive effects of elevated CO2 concentration ([CO2] and other factors, yet the interactive effects of elevated [CO2] and increasing precipitation on maize has remained unclear. In this study, a manipulative experiment in Jinzhou, Liaoning province, Northeast China was performed so as to obtain reliable results concerning the later effects. The Open Top Chambers (OTCs experiment was designed to control contrasting [CO2] i.e., 390, 450 and 550 µmol·mol(-1, and the experiment with 15% increasing precipitation levels was also set based on the average monthly precipitation of 5-9 month from 1981 to 2010 and controlled by irrigation. Thus, six treatments, i.e. C550W+15%, C550W0, C450W+15%, C450W0, C390W+15% and C390W0 were included in this study. The results showed that the irrigation under elevated [CO2] levels increased the leaf net photosynthetic rate (Pn and intercellular CO2 concentration (Ci of maize. Similarly, the stomatal conductance (Gs and transpiration rate (Tr decreased with elevated [CO2], but irrigation have a positive effect on increased of them at each [CO2] level, resulting in the water use efficiency (WUE higher in natural precipitation treatment than irrigation treatment at elevated [CO2] levels. Irradiance-response parameters, e.g., maximum net photosynthetic rate (Pnmax and light saturation points (LSP were increased under elevated [CO2] and irrigation, and dark respiration (Rd was increased as well. The growth characteristics, e.g., plant height, leaf area and aboveground biomass were enhanced, resulting in an improved of yield and ear characteristics except axle diameter. The study concluded by reporting that, future elevated [CO2] may favor to maize when coupled with increasing amount of precipitation in Northeast China.

  6. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  7. Calculation Model of Mass Action Concentration for Mg-Al, Sr-Al and Ba-Al Melts and Determination of Their Thermodynamic Parameters

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Based on the phase diagrams and the mass action law in combination with the coexistence theory of metallic melts structure, the calculation model of mass action concentration for Mg-Al, Sr-Al and Ba-Al was built, and their thermodynamic parameters were determined. The agreement between calculated and measured results shows that the model and the determined thermodynamic parameters can reflect the structural characteristics of relevant melts. However, the fact that the thermodynamic parameters from literature don′t give the value agree with the measured result may be due to unconformity of these parameters to real chemical reactions in metallic melts.

  8. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  9. Changes in visual quality, physiological and biochemical parameters assessed during the postharvest storage at chilling or non-chilling temperatures of three sweet basil (Ocimum basilicum L.) cultivars.

    Science.gov (United States)

    Fratianni, Florinda; Cefola, Maria; Pace, Bernardo; Cozzolino, Rosaria; De Giulio, Beatrice; Cozzolino, Autilia; d'Acierno, Antonio; Coppola, Raffaele; Logrieco, Antonio Francesco; Nazzaro, Filomena

    2017-08-15

    Leaves of three different sweet basil (Ocimum basilicum L.) cultivars (Italico a foglia larga, Cammeo, and Italiano classico) packed in macro-perforated polyethylene bags were stored at chilling (4°C) or non-chilling temperature (12°C) for 9days. During storage, visual quality, physiological (respiration rate, ethylene production, ammonium content) and chemical (antioxidant activity, total polyphenols and polyphenol profile) parameters were measured. Detached leaves stored at chilling temperature showed visual symptoms related to chilling injury, while ethylene production and ammonium content resulted associated to cultivar sensibility to damage at low temperature. Storage at 4°C caused a depletion in polyphenols content and antioxidant capability, which was preserved at 12°C. Regarding the polyphenols profile, stressful storage conditions did not enhance the phenolic metabolism. However, leaves stored at 12°C did not loss a significant amount of metabolites respect to fresh leaves, suggesting the possibility to extend the storability after the expiration date, for a possible recovery of bioactive compounds.

  10. Effect of priming on growth, biochemical parameters and mineral composition of different cultivars of coriander (Coriandrum sativum L. under salt stress

    Directory of Open Access Journals (Sweden)

    Ben Fredj Meriem

    2014-08-01

    Full Text Available At Higher Institute of Agriculture of Chott Mariem, Sousse, Tunisia, this study was conducted to evaluate the interactive effect of salinity and seed priming on coriander. The experiment was carried out in completely randomized design with three replications consisting of four coriander genotypes (Tunisian cv, Algerian cv, Syrian cv and Egyptian cv at two seed conditions (seed priming with 4 g/l NaCl for 12h or no seed priming. Results revealed that seed priming and salinity had significantly (p≤0.05 affected all the parameters under study. On the first hand, salinity stress had adversely affected growth, chlorophyll content, mineral composition (K+ and Ca2+ of coriander in all genotypes. Also, it activated Na+ accumulation and synthesis of proline, soluble sugars and proteins. However, seed priming with NaCl had diminished the negative impact of salt stress in all cultivars and primed plants showed better response to salinity compared to unprimed plants. Maximum values were recorded in tolerant cultivar which is Tunisian one whereas minimum values were noted in sensitive cultivar (Algerian cv.

  11. Effects of different levels of coriander (Coriandrum sativum) seed powder and extract on serum biochemical parameters, microbiota, and immunity in broiler chicks.

    Science.gov (United States)

    Hosseinzadeh, Hesam; Alaw Qotbi, Ali Ahmad; Seidavi, Alireza; Norris, David; Brown, David

    2014-01-01

    The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  12. Haematological and biochemical analysis in canine enteritis

    Directory of Open Access Journals (Sweden)

    Abid Ali Bhat

    Full Text Available Aim: The present investigation screened eighteen clinical cases of canine enteritis for haematological and biochemical analyses. Materials and Methods: Eighteen dogs suffering from enteritis were selected and detailed clinical manifestations were noted. Hematological and biochemical parameters were estimated by using various kits. Blood was also collected from twelve healthy dogs for establishing control values and data obtained were subjected to statistical analysis. Results: The affected dogs showed anorexia, diarrhoea, depression, varying degree of dehydration and tachycardia. There were significant changes in packed cell volume, neutrophils, lymphocytes and mean corpuscular haemoglobin concentration. Biochemical investigation revealed significant decrease in plasma glucose, total plasma protein, albumin and albumin:globulin ratio (A:G ratio. The level of potassium and chloride was markedly decreased. Significant increase in alanine aminotransferase (ALT and blood urea nitrogen (BUN was observed. Conclusion: Packed Cell Volume (PCV and Total Erythrocyte Count (TEC remained almost similar between healthy dogs and dogs affected with diarrhoea. Mean Total Leukocyte Count (TLC value was significantly higher as compared to the control group. Hypoglycemia, hypoproteinemia, hypokalemia, hypochloremia and increase in blood urea nitrogen was observed in dogs suffering from enteritis. [Vet World 2013; 6(7.000: 380-383

  13. Biochemical and histological changes in the liver tissue of rainbow trout (Oncorhynchus mykiss) exposed to sub-lethal concentrations of diazinon.

    Science.gov (United States)

    Banaee, Mahdi; Sureda, Antoni; Mirvaghefi, Ali Reza; Ahmadi, Kamal

    2013-06-01

    The organophosphate insecticide diazinon is widely used to control pest in Iran. The purpose of the present study was to investigate the antioxidant and histopathological changes in the liver tissue of rainbow trout (Oncorhynchus mykiss) exposed to 0.1 and 0.2 mg/L of a commercial formula of diazinon for a period of 28 days. Antioxidant enzyme activities--catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase--were determined after 7, 14 and 28 days of exposure. Histopathological analyses were performed at the 28th day. All antioxidant enzymes were induced after 7 days of diazinon treatment in both concentrations of diazinon. Catalase and superoxide dismutase maintained elevated activities during all the treatment period. Glutathione peroxidase activity returned to the control values at the 14th day, decreasing to values below control at the 28th day in both diazinon concentrations. Glutathione reductase maintained increased activities at the 14th day in the 0.1 mg/L diazinon, decreasing to control values at the 28th day. In the 0.2 mg/L group, the activity returned to control values at the 14th and decreased below the control at the 28th day. Total antioxidant capacity of hepatocytes significantly decreased in fishes exposed to diazinon during all experimental periods. Hypertrophy of hepatocytes, vacuolization of cell cytoplasm and hepatocyte cloudy swelling were observed in the liver tissue of fish exposed to both concentrations of diazinon. The results showed that diazinon altered the activity of antioxidant enzymes and the cellular total antioxidant capacity inducing oxidative stress and cellular damage in hepatocytes evidenced by histopathological analysis.

  14. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: effects on hemolymph and biochemical parameters.

    Science.gov (United States)

    An, Myung In; Choi, Cheol Young

    2010-01-01

    Changes in water temperature and salinity are responsible for a variety of physiological stress responses in aquatic organisms. Stress induced by these factors was recently associated with enhanced reactive oxygen species (ROS) generation, which caused oxidative damage. In the present study, we investigated the time-related effects of changes in water temperature and salinity on mRNA expression and the activities of antioxidant enzymes (SOD and CAT) and lipid peroxidation (LPO) in the gills and digestive glands of the ark shell, Scapharca broughtonii. To investigate physiological responses, hydrogen peroxide (H(2)O(2)), lysozyme activity, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT) were measured in the hemolymph. Water temperature and salinity changes significantly increased antioxidant enzyme mRNA expression and activity in the digestive glands and gills in a time-dependent manner. H(2)O(2) concentrations increased significantly in the high-temperature and hyposalinity treatments. LPO, AspAT and AlaAT levels also increased significantly in a time-dependent manner, while lysozyme activity decreased. These results suggest that antioxidant enzymes play important roles in reducing oxidative stress in ark shells exposed to changes in water temperature and salinity.

  15. Effect of fishmeal replacement by soy protein concentrate with taurine supplementation on growth performance, hematological and biochemical status, and liver histology of totoaba juveniles (Totoaba macdonaldi).

    Science.gov (United States)

    López, Lus M; Flores-Ibarra, Maricela; Bañuelos-Vargas, Isaura; Galaviz, Mario A; True, Conal D

    2015-08-01

    The effect of dietary inclusion of soy protein concentrate (SPC) and simultaneous supplementation with taurine on the growth, hematology, blood biochemistry, and liver histology of totoaba (Totoaba macdonaldi) juveniles was assessed. Four isoproteic and isolipidic diets were formulated containing either 30 or 60% of SPC (diets S30 and S60), supplemented or not with 1% of taurine (diets S30T and S60T). A fishmeal-based diet formulated for totoaba nutritional requirements, without SPC and taurine supplementation, was used as a reference diet. Triplicate groups of 32 totoaba juveniles (average body weight 7.5 ± 0.6 g) were fed these diets for 45 days. Results showed that growth performance in fish fed S30, S30T, and S60T was similar to fish fed the reference diet. Red blood cells and hematocrit in fish fed with supplemented taurine in both levels of SPC (S30T and S60T) were similar to the fish fed the RD; the addition of taurine improved the state of hydration of totoaba. Plasmatic hemoglobin in fish fed the lower SPC level was similar to fish fed the RD. The mean corpuscular hemoglobin concentration in fish fed S30T was similar to fish fed the RD, taurine supplementation prevented the development of hypochromic anemia in this group of fish. Plasmatic albumin in fish fed S30 was similar to fish fed the RD. Plasmatic total protein and globulin concentration increased and AL:GLB (albumin:globulin ratio) decreased in fish fed the SPC-based diets despite taurine supplementation. The protein profile showed that taurine supplementation did not prevent a possible inflammatory process (increased globulins, decreased AL:GLB) in juvenile totoaba fed both levels of SPC. Glucose concentration was similar in fish fed S30, S30T, and S60T. The histological hepatic index was highest in fish fed S60. These results suggest that with an appropriate nutritional level, taurine may play an important modulatory role in the hematology and blood biochemistry status in totoaba fed SPC

  16. Trans fat intake across gestation and lactation increases morphine preference in females but not in male rats: Behavioral and biochemical parameters.

    Science.gov (United States)

    Roversi, Karine; Pase, Camila Simonetti; Roversi, Katiane; Vey, Luciana Taschetto; Dias, Verônica Tironi; Metz, Vinícia Garzella; Burger, Marilise Escobar

    2016-10-05

    The abuse of morphine has risen considerably in recent years, mainly due to the increase of its prescription in clinical medicine. Also, increased consumption of processed foods, rich in trans fatty acids (TFA), has caused concerns about human health. Thus, the aim of our study was to determine whether trans fat consumption in the perinatal period may affect preference for morphine in adolescent female and male rats. Dams were orally supplemented with water (C-control) or hydrogenated vegetable fat (HVF-rich in TFA) during gestation and lactation periods. On post-natal day 43, pups were exposed to morphine (4mg/kg i.p., for 4 days) and assessed in the conditioned place preference paradigm. Anxiety-like symptoms were assessed, and oxidative status of the brain was estimated by reactive species (RS) generation. Female rats with HVF supplementation showed increased morphine preference and less anxiety-like symptoms. Additionally, both male and female rats from HVF-supplementation showed increased RS generation in the ventral tegmental area, whose level was similar in morphine-conditioned female rats. RS generation was increased in the hippocampus of morphine-conditioned female rats, regardless of the supplementation of their dams. We may infer that gender is a predictive factor to opioid preference, since adolescent female rats showed more susceptibility to addiction than males. Furthermore, trans fat consumption across the perinatal period is able to modify parameters of opioid preference in female rats, possibly due to TFA incorporation in phospholipid membranes, modifying the endogenous opioid system and the oxidative status in brain areas related to drug addiction.

  17. Effects of PCSK1 genetic variants on obesity among Thai children and their family members: in relation to health risk, and biochemical and anthropometric parameters.

    Science.gov (United States)

    Kulanuwat, Sirikul; Phonrat, Benjaluck; Tungtrongchitr, Anchalee; Limwongse, Chanin; Chongviriyaphan, Nalinee; Tungtrongchitr, Rungsunn; Santiprabhob, Jeerunda

    2014-01-01

    Single nucleotide polymorphisms (SNPs) in PCSK1, namely, rs6234, rs6235, and rs271939 have been linked to obesity in European population; and rs3811951 has also been connected to type 2 diabetes and obesity parameters in Chinese population. In this family-based case-control study, we analyzed links between PCSK1 genetic variants and obesity in Thai children and their families. Eleven obese children with a percent weight for height > or = 140 who had family history of obesity and 69 family members were recruited. SNPs rs6234, rs6235, rs3811951, and rs271939 of PCSK1 were analyzed using PCR and gene sequencing methods. DNA of 200 normal weight subjects was used as control. Participants with variant genotypes in the rs6234-6235 pair are at significantly more risk of being obese [OR = 2.44 (1.35-4.43), p = 0.003], and also at increased risk of being severely obese (obese class III) [OR = 3.03 (1.20-7.66), p = 0.015]. Variant rs3811951 showed no association with being obese, but is significantly linked to an increased risk of being severely obese [OR = 3.59 (1.42-9.08) p = 0.005]. Moreover, high density lipoprotein (HDL)-C levels between normal and variant rs3811951 group differed considerably, with patients with variant genotype having a lower HDL-C level (p = 0.037). Thus, Thais carrying SNPs rs6234-5 are at increased risk of being obese, and the risk of severe obesity increases when carrying both rs6234-5 and rs3811951, but not with rs271939. Furthermore, patients with genetic variations at rs3811951 are at risk of having low HDL-C levels.

  18. Effects of food processing and fibre content on the digestibility, energy intake and biochemical parameters of Blue-and-gold macaws (Ara ararauna L. - Aves, Psittacidae).

    Science.gov (United States)

    Veloso, R R; Sakomura, N K; Kawauchi, I M; Malheiros, E B; Carciofi, A C

    2014-04-01

    Considering the increased incidence of obesity and metabolic diseases in caged psittacines, the effect of fibre and food processing was evaluated in the Blue-and-gold macaw. Four food formulations (0%, 7%, 14% and 21% of sugarcane fibre) processed by pelleting or extrusion were studied, resulting in eight diets. To study digestibility, 48 macaws housed in pairs in cages was used in a block design. Subsequently, diets containing 0% or 21% sugarcane fibre, pelleted or extrude was fed for 4 months to evaluate energy intake and blood metabolites. A 2 × 2 × 2 (two fibre levels, two food processing methods and two genders) factorial arrangement with subplots (beginning and end) was used. When differences were detected in anova's F test, data were submitted to polynomial contrasts in the first experiment and to orthogonal contrasts in the second experiment (p food processing. Pelleted foods presented higher dry matter digestibility and food metabolisable energy (ME) than the extruded ones (p processing did not change ME ingestion (p > 0.05). The macaws gained body weight (p 0.05), but females fed with the high-fibre diets did not gain weight (p > 0.05), suggesting a low food ME (12.5 kJ/g).The substitution of the original diet (sunflower seeds, fruits and cooked maize) by the experimental foods decreased the basal (12-h fast) concentrations of glucose, cholesterol and triglycerides (p < 0.001). The consumption of pelleted diets reduced serum glucose and cholesterol (p < 0.05). Results suggest that the pelleted diets were more beneficial and can be used to reduce blood metabolites related to metabolic disorders that are commonly observed in macaws.

  19. The effects of palm oil tocotrienol-rich fraction supplementation on biochemical parameters, oxidative stress and the vascular wall of streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Siti Balkis Budin

    2009-03-01

    Full Text Available OBJECTIVE: This study examined the effects of palm oil tocotrienol-rich fractions on streptozotocin-induced diabetic rats. METHODS: Animals were divided into three groups: (i normal non-diabetic (NDM, (ii diabetic treated (tocotrienol-rich fractions - TRF and (iii diabetic untreated (non-TRF. The treatment group received oral administration of tocotrienol-rich fractions (200 mg/kg body weight daily for eight weeks. The normal non-diabetic and the diabetic untreated groups were fed standard rat feed. Blood glucose and lipid profiles, oxidative stress markers and morphological changes of the thoracic aorta were evaluated. RESULTS: Tocotrienol-rich fractions treatment reduced serum glucose and glycated hemoglobin concentrations. The tocotrienol-rich fractions group also showed significantly lower levels of plasma total cholesterol, low-density lipoprotein cholesterol, and triglyceride, as compared to the untreated group. The tocotrienol-rich fractions group had higher levels of high-density lipoprotein cholesterol, as compared to the untreated group. Superoxide dismutase activity and levels of vitamin C in plasma were increased in tocotrienol-rich fractions-treated rats. The levels of plasma and aorta malondealdehyde + 4-hydroxynonenal (MDA + 4-HNE and oxidative DNA damage were significant following tocotrienol-rich fractions treatment. Electron microscopic examination showed that the normal morphology of the thoracic aorta was disrupted in STZ-diabetic rats. Tocotrienol-rich fractions supplementation resulted in a protective effect on the vessel wall. CONCLUSION: These results show that tocotrienol-rich fractions lowers the blood glucose level and improves dyslipidemia. Levels of oxidative stress markers were also reduced by administration of tocotrienol-rich fractions. Vessel wall integrity was maintained due to the positive effects mediated by tocotrienol-rich fractions.

  20. Experimental and statistical determination of indicator parameters for the evaluation of fly ash and boiler ash PCDD/PCDF concentration from municipal solid waste incinerators.

    Science.gov (United States)

    Streibel, T; Nordsieck, H; Neuer-Etscheidt, K; Schnelle-Kreis, J; Zimmermann, R

    2007-04-01

    On-line detectable indicator parameters in the flue gas of municipal solid waste incinerators (MSWI) such as chlorinated benzenes (PCBz) are well known surrogate compounds for gas-phase PCDD/PCDF concentration. In the here presented work derivation of indicators is broadened to the detection of fly and boiler ash fractions with increased PCDD/PCDF content. Subsequently these fractions could be subject to further treatment such as recirculation in the combustion chamber to destroy their PCDD/PCDF and other organic pollutants' content. Aim of this work was to detect suitable on-line detectable indicator parameters in the gas phase, which are well correlated to PCDD/PCDF concentration in the solid residues. For this, solid residues and gas-phase samples were taken at three MSWI plants in Bavaria. Analysis of the ash content from different plants yielded a broad variation range of PCDD/PCDF concentrations especially after disturbed combustion conditions. Even during normal operation conditions significantly increased PCDD/PCDF concentrations may occur after unanticipated disturbances. Statistical evaluation of gas phase and ash measurements was carried out by means of principal component analysis, uni- and multivariate correlation analysis. Surprisingly, well known indicators for gas-phase PCDD/PCDF concentration such as polychlorinated benzenes and phenols proved to be insufficiently correlated to PCDD/PCDF content of the solid residues. Moreover, no single parameter alone was found appropriate to describe the PCDD/PCDF content of fly and boiler ashes. On the other hand, multivariate fitting of three or four parameters yielded convenient correlation coefficients of at least r=0.8 for every investigated case. Thereby, comprehension of plant operation parameters such as temperatures and air flow alongside concentrations of inorganic compounds in the gas phase (HCl, CO, SO2, NOx) gave the best results. However, the suitable set of parameters suited best for estimation of

  1. Quantifying biochemical variables of corn by hyperspectral reflectance at leaf scale

    Institute of Scientific and Technical Information of China (English)

    Qiu-xiang YI; Jing-feng HUANG; Fu-min WANG; Xiu-zhen WANG

    2008-01-01

    To further develop the methods to remotely sense the biochemical content of plant canopies,we report the results of an experiment to estimate the concentrations of three biochemical variables of corn,i.e.,nitrogen (N),crude fat (EE) and crude fiber (CF) concentrations,by spectral reflectance and the first derivative reflectance at fresh leaf scale.The correlations between spectral reflectance and the first derivative transformation and three biochemical variables were analyzed,and a set of estimation models were established using curve-fitting analyses.Coefficient of determination (R2),root mean square error (RAISE) and relative error of prediction (PEP) of estimation models were calculated for the model quality evaluations,and the possible opti-mum estimation models of three biochemical variables were proposed,with R2 being 0.891,0.698 and 0.480 for the estimation models of N,EE and CF concentrations,respectively.The results also indicate that using the first derivative reflectance was better than using raw spectral reflectance for all three biochemical variables estimation,and that the first derivative reflectances at 759 nm,1954 nm and 2370 nm were most suitable to develop the estimation models of N,EE and CF concentrations,respectively.In addition,the high correlation coefficients of the theoretical and the measured biochemical parameters were obtained,especially for nitrogen (r=0.948).

  2. [Effect of stress actions on some hematologic and biochemical parameters of rat blood and on energetic intermolecular interactions in lipid extract under effect of light radiation].

    Science.gov (United States)

    Zabelinskiĭ, S A; Chebotareva, M A; Tavrovskaia, T V; Skverchinskaia, E A; Shukoliukova, E P; Maslov, M N; Krivchenko, A I

    2012-01-01

    Comparative study has been carried of effect of the three-day long starvation, running, and their combination on morphological parameters of rat blood, lipid metabolism, and activity of blood Na,K-ATPase. Different effect has been shown of these stress factors on the blood erythrocyte composition. Starvation is accompanied by the most pronounced release of stored erythrocyte into blood, which results in a significant decrease both of the total amount of reticulocytes and the complete absence of reticulocytes of the I stage of maturity (the youngest). The running on treadmill led to a significant increase of the total amount of blood reticulocytes and to multiple increase of immature reticulocytes (RC-I and RC-II), which can indicate some stress of the bone marrow erythroid stem line. The curve of acid resistance of blood reticulocytes has shown the animal to experience the greatest stress at a combination of starvation and running. Starvation and running produced different effects on blood lipid characteristics. The content of triacylglycerides (TAG) in blood rose by 40% at starvation and decreased by 30% at running, a similar tendency being found for index of atherogeneity. The fatty acid composition of blood phospholipids at running and its combination with starvation practically did not differ from control. A change of Na,K-ATPase, which is so characteristic of reaction to various kinds of stress, sharply fell at starvation (by 22%), but increased at running (by 13%) and decreased markedly at combination of these actions. Absorption spectra of lipid extracts of the whole blood of the rats submitted to various stress actions showed that extracted from blood (at different amount depending on the kind of action) is an organic substance with coupled bonds, which absorbs light in the diapason of 360-620 nm. The absorption of light in the diapason of 400-410 nm has been found to belong to the Soret band of ferroheme and ferriheme. The shift of the Soret band indicates

  3. Elevated circulating plasma endothelin-1 concentrations in cirrhosis

    DEFF Research Database (Denmark)

    Møller, S; Emmeluth, C; Henriksen, Jens Henrik Sahl

    1993-01-01

    As endothelin-1 (ET-1), a potent vasoconstricting peptide, may play a role in the circulatory derangement and renal impairment in cirrhosis, the aim of the present study was to investigate plasma concentrations of ET-1 in different vascular beds in relation to clinical and biochemical parameters.......70, P plasma concentration between the liver, renal, or femoral...

  4. Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations

    Directory of Open Access Journals (Sweden)

    MacNeill Stuart A

    2006-11-01

    Full Text Available Abstract Background DNA ligases are required for DNA strand joining in all forms of cellular life. NAD+-dependent DNA ligases are found primarily in eubacteria but also in some eukaryotic viruses, bacteriophage and archaea. Among the archaeal NAD+-dependent DNA ligases is the LigN enzyme of the halophilic euryarchaeon Haloferax volcanii, the gene for which was apparently acquired by Hfx.volcanii through lateral gene transfer (LGT from a halophilic eubacterium. Genetic studies show that the LGT-acquired LigN enzyme shares an essential function with the native Hfx.volcanii ATP-dependent DNA ligase protein LigA. Results To characterise the enzymatic properties of the LigN protein, wild-type and three mutant forms of the LigN protein were separately expressed in recombinant form in E.coli and purified to apparent homogeneity by immobilised metal ion affinity chromatography (IMAC. Non-isotopic DNA ligase activity assays using λ DNA restriction fragments with 12 bp cos cohesive ends were used to show that LigN activity was dependent on addition of divalent cations and salt. No activity was detected in the absence of KCl, whereas maximum activity could be detected at 3.2 M KCl, close to the intracellular KCl concentration of Hfx.volcanii cells. Conclusion LigN is unique amongst characterised DNA ligase enzymes in displaying maximal DNA strand joining activity at high (> 3 M salt levels. As such the LigN enzyme has potential both as a novel tool for biotechnology and as a model enzyme for studying the adaptation of proteins to high intracellular salt levels.

  5. The activity concentrations of 222Rn and corresponding health risk in groundwater samples from basement and sandstone aquifer; the correlation to physicochemical parameters

    Science.gov (United States)

    Abdurabu, Wedad Ali; Ramli, Ahmad Termizi; Saleh, Muneer Aziz; Heryansyah, Arien

    2016-10-01

    This study aims to evaluate the activity concentrations of 222Rn and to assess the corresponding health risk in groundwater samples obtained in Juban District, Ad Dali' Governorate, Yemen. The measurements were performed by RAD 7 radon detector manufactured by DURRIDGE COMPANY Inc. The activity concentrations of 222Rn ranged from 1.0±0.2 Bq l-1 to 896.0±0.8 Bq l-1. 57% of the groundwater samples were above the US Environmental Protection Agency (USEPA) recommended value for Rn in water. Induced coupled plasma mass spectrometry (ICP-MS) was used to determine the concentrations of uranium in groundwater samples. The measured concentration of U ranged from 0.33±0.01 μg l-1 to 24.6±0.6 μg l-1. The results were comparable to internationally recommended values. The highest concentration of U and 222Rn were found to be in the basement aquifer, while the lowest concentrations of both radionuclides were in the sandstone aquifer. High concentrations of Rn are found along fault zones. The relationship between the activity concentration of 222Rn, concentration of U and physicochemical parameters were investigated. The results showed a very strong relationship between activity concentrations of 222Rn with concentrations of U and the salinity of water.

  6. 不育男性精浆生化标志物与精液参数分析%Analyse of biochemical marker in seminal plasma and sperm parameters of infertile male

    Institute of Scientific and Technical Information of China (English)

    何雁; 王岚; 高婷; 伏建峰; 刘俊

    2011-01-01

    Objective: To investigate the important role of biochemical marker of seminal plasma in male fertility evaluation, and the relationship between biochemical marker and sperm parameters. Methods: The activities of zinc and acid phosphatase in seminal plasma were routinely detected in 97 infertile men and 30 normal fertility as control. We carried out the biochemical detection of seminal plasma, including fructose content, acid phosphatase content, α - glycosidase content and zinc content. The data was be statistically analysed by SPSS - 11.0 software. Results: ①The seminal plasma fructose content of anormal sperm density in infertile group is significant higher than normal sperm density in infertile group or normal fertility group ( P < 0. 05 ); The seminal plasma fructose content of anormal sperm activity in infertile group have significant difference than normal fertility group ( P < 0.05 ). ② The seminal plasma acid phosphatase content of anormal sperm activity in infertile group is significant lower than normal sperm activity in infertile group or normal fertility group ( P < 0.05 ); The seminal plasma acid phosphatase content of anormal sperm density in infertile group have no statistical difference with normal sperm density in infertile group or normal fertility group ( P > 0.05 ). ③The seminal plasma α - glycosidase content of anormal sperm density in infertile group is significant lower than normal sperm density in infertile group or normal fertility group (P <0.05); The seminal plasma α - glycosidase content nf anormal sperm activity in infertile group is significant lower than normal sperm activity in infertile group or normal fertility group ( P < 0.05 ). ④The seminal plasma zinc content of anormal sperm density in infertile group is significant lower than normal sperm density in infertile group or normal fertility group (P <0.01 ); The seminal plasma zinc content of anormal sperm activity in infertile group have no statistical

  7. Effect of concentration, temperature, and freezing rate on the phosphorescence parameters of acenaphthene in n-hexane matrices

    Science.gov (United States)

    Zhdanova, N. V.; Deryabin, M. I.

    2016-04-01

    The effect of the concentration, temperature, and freezing rate on the spectra, kinetics, and intensity of phosphorescence of acenaphthene in polycrystalline n-hexane matrices has been studied. The specific features of the transformation of quasi-lines in the phosphorescence spectrum of molecules embedded in an n-hexane crystal lattice into broad molecular bands at high concentrations of the solution (~10‒1 mol/L) have been considered. It has been demonstrated that a distortion of the n-hexane crystal lattice at a high concentration of embedded acenaphthene molecules can be a cause of the observed transformation of the quasi-line spectrum.

  8. Spray pyrolysed Cu2ZnSnS4/In2S3 thin film solar cell: Effect of varying copper concentration on cell parameters

    Science.gov (United States)

    Menon, M. R. Rajesh; Rajeshmon, V. G.; Thomas, Titu; Kartha, C. Sudha; Vijayakumar, K. P.

    2016-05-01

    A double layer Cu2ZnSnS4 absorber was employed for the first time to improve the performance of spray pyrolysed Cu2ZnSnS4/In2S3 thin film solar cell. Copper concentration in the two layers of Cu2ZnSnS4 was adjusted and effect on performance parameters was studied. It was observed that higher copper concentration in the absorber layer adjacent to the electrode is beneficial for device performance, whereas, lower copper concentration in absorber layer near to the junction has detrimental effect on the device properties.

  9. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits.

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Ni, Hengjia; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu; Fang, Jun

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  10. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Directory of Open Access Journals (Sweden)

    Maidelys Iser

    2016-01-01

    Full Text Available This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment, which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum, serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P<0.05 the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P<0.05 the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P<0.05 when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P<0.05 the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits.

  11. Comparison of parameters influencing the behavior of concentration of nitrates and phosphates during different extreme rainfall-runoff events in small watersheds

    Directory of Open Access Journals (Sweden)

    J. Moravcová

    2013-10-01

    Full Text Available The behavior of solute concentrations during storm events is completely different from their behaviour under normal conditions, and very often results in hysteresis. This study aim is to explore the relationship between the biogeochemical and hydrological parameters describing natural conditions and the reciprocal interactions between changes in concentration of selected indicators of water quality in water and the discharge dynamics during different types of extreme rainfall-runoff events in the Jenínský stream and the Kopaninský stream catchment (Czech Republic. The relationship between concentrations and runoffs is explained by concentration-discharge hysteretic loops. As the statistical method used for cross analyzing the impact of the parameters there was chosen the RDA analysis. The relationships between the particular parameters were examined separately by conditions of spring snow melt and summer storm events. The results than confirmed the very strong relationship between parameters describing water quality and percentage of stable parts of the catchment and also of infiltration vulnerable sites.

  12. The Effects of Agave fourcroydes Powder as a Dietary Supplement on Growth Performance, Gut Morphology, Concentration of IgG, and Hematology Parameters in Broiler Rabbits

    Science.gov (United States)

    Iser, Maidelys; Martínez, Yordan; Jiang, Hongmei; Valdivié Navarro, Manuel; Wu, Xiaosong; Al-Dhabi, Naif Abdullah; Rosales, Manuel; Duraipandiyan, Veeramuthu

    2016-01-01

    This study was conducted to determine the effects of Agave fourcroydes powder as a dietary supplement on the growth performance, gut morphology, serum concentration of IgG, and the hematology parameters of broiler rabbits. A total of 32 rabbits [New Zealand × Californian] were weaned at 35 days. They were randomly selected for two dietary treatments (eight repetitions per treatment), which consisted of a basal diet and a basal diet supplemented with 1.5% dried-stem powder of A. fourcroydes. On day 60 from the initiation of treatment, gut histomorphology (duodenum and cecum), serum concentration of IgG, and hematology parameters were all measured. The results showed that A. fourcroydes powder supplementation improved (P < 0.05) the ADFI, ADG, and final BW. Correspondingly, this treatment increased (P < 0.05) the muscle and mucosa thickness and height and width of villi. However, duodenum crypts depth was lower (P < 0.05) when rabbits were fed with this natural product, compared with the basal diet treatment. Results also indicated that the A. fourcroydes powder increased (P < 0.05) the serum concentration of IgG but did not change the hematology parameters. This data indicates that A. fourcroydes powder, as a supplement, had beneficial effects on increasing the growth performance and serum concentration of IgG, as well as improving the gut morphology without affecting the hematology parameters in broiler rabbits. PMID:27777945

  13. Effect of f Noise Stress on Blood Biochemical Parameters of Laying Hens%噪声应激对蛋鸡血液生化指标的影响

    Institute of Scientific and Technical Information of China (English)

    苏燕芳; 齐大胜; 韦彩利; 程丽丽; 杨松; 陈辉; 黄仁录

    2011-01-01

    The objective of this research was to study the effect of noise stress on blood biochemical parameters of hens. At the age of 500 day, 120 Hyline (Brown) commercial hens were randomly assigned to 3groups, with four rep- lications per group, 10 chickens in%500日龄海兰褐商品蛋鸡120只,随机分为3组,每组4个重复,每个重复10只鸡。研究噪声应激对蛋鸡血液生化指标的影响。结果表明,噪声对甘油三酯、葡萄糖、钙、磷及总胆固醇含量及肌酸激酶、乳酸脱氢酶、碱性磷酸酶、丙氨酸氨基转移酶活性影响差异不显著(P〉0.05)。日粮中添加60IU/kg的维生素E对缓解应激起到一定作用(P〉0.05)。

  14. The influence of soil organic carbon on interactions between microbial parameters and metal concentrations at a long-term contaminated site

    Energy Technology Data Exchange (ETDEWEB)

    Muhlbachova, G. [Crop Research Institute, Drnovska 507, 161 06 Prague 6, Ruzyne (Czech Republic); Sagova-Mareckova, M., E-mail: sagova@vurv.cz [Crop Research Institute, Drnovska 507, 161 06 Prague 6, Ruzyne (Czech Republic); Omelka, M. [Charles University, Faculty of Mathematics and Physics, Dept. of Probability and Mathematical Statistics, Prague 8, Karlin (Czech Republic); Szakova, J.; Tlustos, P. [Czech University of Life Sciences, Department of Agroenvironmental Chemistry and Plant Nutrition, Prague 6, Suchdol (Czech Republic)

    2015-01-01

    The effects of lead, zinc, cadmium, arsenic and copper deposits on soil microbial parameters were investigated at a site exposed to contamination for over 200 years. Soil samples were collected in triplicates at 121 sites differing in contamination and soil organic carbon (SOC). Microbial biomass, respiration, dehydrogenase activity and metabolic quotient were determined and correlated with total and extractable metal concentrations in soil. The goal was to analyze complex interactions between toxic metals and microbial parameters by assessing the effect of soil organic carbon in the relationships. The effect of SOC was significant in all interactions and changed the correlations between microbial parameters and metal fractions from negative to positive. In some cases, the effect of SOC was combined with that of clay and soil pH. In the final analysis, dehydrogenase activity was negatively correlated to total metal concentrations and acetic acid extractable metals, respiration and metabolic quotient were to ammonium nitrate extractable metals. Dehydrogenase activity was the most sensitive microbial parameter correlating most frequently with contamination. Total and extractable zinc was most often correlated with microbial parameters. The large data set enabled robust explanation of discrepancies in organic matter functioning occurring frequently in analyzing of contaminated soil processes. - Highlights: • Soil organic carbon affected all interactions between metals and microorganisms. • Soil organic carbon adjustment changed correlations from positive to negative. • Ammonium nitrate extractable metals were the most influencing fraction. • Dehydrogenase activity was the most affected soil parameter. • Zinc was the most toxic metal among studied metals.

  15. 全自动生化仪与快速血糖仪测定血糖浓度的比较%Comparison between automatic biochemical analyzer and rapid blood sugar meter for determining glucose blood concen-tration

    Institute of Scientific and Technical Information of China (English)

    刘兴高; 成平

    2015-01-01

    目的:探讨全自动生化仪测定静脉促凝血清标本血糖浓度与快速血糖仪测定静脉抗凝全血标本血糖浓度的相关性及差异。方法采集该院200名住院患者的血液标本,同一患者同时采集静脉促凝血清标本与静脉抗凝全血标本,采用全自动生化仪测定静脉促凝血清标本血糖浓度,同时采用快速血糖仪测定静脉抗凝全血标本血糖浓度,比较2组血糖浓度测定结果。结果191名患者的静脉促凝血清标本血糖浓度明显大于其静脉抗凝全血标本血糖浓度,差异范围在0.50~1.50 mmol/L ,差异有统计学意义(P<0.05)。结论全自动生化仪测定静脉促凝血清标本血糖浓度明显高于快速血糖仪测定静脉抗凝全血标本的血糖浓度。%Objective To investigate the correlation and difference between the automatic biochemical analyzer for determining the blood glucose concentration in the venous coagulant serum specimens and the fast blood glucose meter for determining the blood glucose concentration in the venous coagulant whole blood specimens .Methods The blood specimens of 200 inpatients in the Geleshan Branch Hospital were collected ,venous coagulant serum specimens and the venous coagulant whole blood specimens in the same patient were simultaneously collected .The automatic bi‐ochemical analyzer was adopted to detect the blood glucose concentration of venous coagulant serum specimens ,and at the same time the rapid blood glucose meter was adopted to detect the blood glucose concentration of venous coagu‐lant whole blood specimens .The blood glucose detection results were compared between the two detection methods . Results In 191 patients ,the blood glucose concentration of venous coagulant serum specimens was significantly grea‐ter than that of the venous coagulant whole blood specimens ,the difference range was 0 .50 -1 .50 mmol/L ,the difference was statistically significant (P<0 .05

  16. Effects of oxygen concentration and body weight on maximum feed intake, growth and hematological parameters of Nile tilapia, Oreochromis niloticus

    NARCIS (Netherlands)

    Tran Duy, A.; Schrama, J.W.; Dam, van A.A.; Verreth, J.A.J.

    2008-01-01

    Feed intake and satiation in fish are regulated by a number of factors, of which dissolved oxygen concentration (DO) is important. Since fish take up oxygen through the limited gill surface area, all processes that need energy, including food processing, depend on their maximum oxygen uptake capacit

  17. Variation of free asparagine concentration and association with quality parameters for hard red spring wheat grown in North Dakota

    Science.gov (United States)

    Free asparagine in wheat is known to be a precursor for the formation of acrylamide which is unacceptable to consumers due to its potential risks to human health. This research was performed to determine variation of free asparagine concentration (FAC) in hard red spring (HRS) wheat grown in North ...

  18. Eccentric and concentric loading of the triceps surae: an in vivo study of dynamic muscle and tendon biomechanical parameters.

    Science.gov (United States)

    Chaudhry, Saira; Morrissey, Dylan; Woledge, Roger C; Bader, Dan L; Screen, Hazel R C

    2015-04-01

    Triceps surae eccentric exercise is more effective than concentric exercise for treating Achilles tendinopathy, however the mechanisms underpinning these effects are unclear. This study compared the biomechanical characteristics of eccentric and concentric exercises to identify differences in the tendon load response. Eleven healthy volunteers performed eccentric and concentric exercises on a force plate, with ultrasonography, motion tracking, and EMG applied to measure Achilles tendon force, lower limb movement, and leg muscle activation. Tendon length was ultrasonographically tracked and quantified using a novel algorithm. The Fourier transform of the ground reaction force was also calculated to investigate for tremor, or perturbations. Tendon stiffness and extension did not vary between exercise types (P = .43). However, tendon perturbations were significantly higher during eccentric than concentric exercises (25%-40% higher, P = .02). Furthermore, perturbations during eccentric exercises were found to be negatively correlated with the tendon stiffness (R2 = .59). The particular efficacy of eccentric exercise does not appear to result from variation in tendon stiffness or extension within a given session. However, varied perturbation magnitude may have a role in mediating the observed clinical effects. This property is subject-specific, with the source and clinical time-course of such perturbations requiring further research.

  19. Introducing enzyme selectivity as a quantitative parameter to describe the effects of substrate concentration on protein hydrolysis

    NARCIS (Netherlands)

    Butré, C.I.

    2014-01-01

    To understand the differences in peptide composition that result from variations in the conditions of enzymatic hydrolysis of proteins (e.g. substrate concentration) the mechanism of hydrolysis needs to be understood in detail. Therefore, methods and tools were developed to characterize and quantify

  20. Effect of nutritional level on nutrient apparent digestibility and serum biochemical parameters of beef calves%营养水平对肉犊牛营养物质消化率和血清指标的影响

    Institute of Scientific and Technical Information of China (English)

    郭峰; 屠焰; 杜红芳; 陈仁杰; 刁其玉

    2015-01-01

    研究不同能量、蛋白质水平的代乳品对南方地区哺乳期肉用犊牛营养物质消化和血清指标的影响,进而确立代乳品能量和蛋白质参数,为南方地区肉犊牛的饲料配制提供数据支持.采用2×2因子设计,犊牛代乳品的能量水平为16、14 MJ/kg,粗蛋白水平为25%、22%,共计4种.选取新生犊牛40头,21日龄断母乳并根据日龄和体重一致原则分为4个处理组,每组10头犊牛,分别饲喂上述4种代乳品,于77~85日龄进行消化试验并静脉采集血液.结果显示相同能量水平,犊牛对代乳品中粗脂肪和钙的表观消化率随粗蛋白水平的升高而提高(P0.05).高能量水平(DE:16 MJ/kg)有助于犊牛粗蛋白质消化吸收,高蛋白水平(CP:25%)有助于犊牛对粗脂肪和钙的利用,低能低蛋白水平(DE:14 MJ/kg ,CP:22%)日粮不利于犊牛营养物质消化吸收.%The objective of this study is to determine the effect of different energy and protein level of milk replacer (MR) on nutrient digestibility and serum biochemical parameters of beef calves. It will establish the energy and protein parameter of milk replacer, providing technical guide for formulating of milk replacer recipes specialized for southern China. The experiment was 2 × 2 factorial design. The crude protein of milk replacers were 22% and 25% and the digestible energy level of milk replacers were 14 and 16 MJ/kg, respectively. Forty neonatal German yellow×Xianan calves were allotted to four treatments (10 per treatment) according to the body weight and age. Calves in all treatments were weaned off whole milk at 21 days of age, followed by the feeding of four different types of milk replac-er till 90 days of age. Digestive experiments were conducted at 77~85 age phase meanwhile blood was sampled to determine biochemical parameters. The results demonstrated that the apparent digestibility of both crude fat and Ca increased in high protein treatments (P0.05). To sum up, the high

  1. 中药复方对热应激下蛋鸡生化指标的影响%Effects of Compound Prescription of Chinese Herbal Medicine on the Biochemical Parameters of Laying Chicken under Heat Stress

    Institute of Scientific and Technical Information of China (English)

    高桂生; 高光平; 史秋梅; 张艳英; 沈萍; 贾雪霞; 张雪莲

    2012-01-01

    [ Objective] The research aimed to discuss the biochemical indices of laying chicken under heat stress. [ Method] 88-day-old laying chicken with the same body weight were randomly divided into five groups, namely prescription ① group, prescription ② group, Vc group, blank group(no drug group of heat stress) and normal group (no heat stress group). The effects of different Chinese herbal compound on the blood biochemical indices of laying chicken under heat stress were studied. [ Result] Heat stress reduced the contents of sodium, potassium, chloride and phosphorus in blank group, and increased the levels of triglyceride and cholesterol. The contents of sodium, potassium, chloride and phosphorus in prescription ① group and prescription ② group all obviously increased, the contents of triglyceride and cholesterol decreased and the concentrations of ALT, LDH, CK were decreased- The effects of prescription ① group and prescription ② group were more obvious than that of Vc group and the effect of prescription ② group was the most signficiant. [Conclusion] Compound prescription of Chinese medicine had a significant effect on alleviating the heat stress of laying chicken.%[目的]探讨热应激下蛋鸡的生化机制.[方法]将88日龄体重大小均匀一致的蛋鸡随机分为5组,即方剂①组、方剂②组、VC组、空白组(热应激无药组)、正常组(无热应激组),研究不同中药复方对热应激状态下蛋鸡血液生化指标的影响.[结果]热应激降低了空白组蛋鸡血钠、血钾、血氯、血磷的含量,并使甘油三酯、胆固醇的含量上升.方剂①组和方剂②组血钠、血钾、血氯、血磷的含量均有明显回升,且能降低甘油三酯、胆固醇的含量,降低血清谷丙转氨酶、谷草转氨酶、乳酸脱氢酶和肌酸激酶的含量.方剂①组、方剂②组均比VC组效果明显,但方剂②组效果最为显著.[结论]中药复方对缓解蛋鸡的热应激具有显著功效.

  2. The influence of soil organic carbon on interactions between microbial parameters and metal concentrations at a long-term contaminated site.

    Science.gov (United States)

    Muhlbachova, G; Sagova-Mareckova, M; Omelka, M; Szakova, J; Tlustos, P

    2015-01-01

    The effects of lead, zinc, cadmium, arsenic and copper deposits on soil microbial parameters were investigated at a site exposed to contamination for over 200 years. Soil samples were collected in triplicates at 121 sites differing in contamination and soil organic carbon (SOC). Microbial biomass, respiration, dehydrogenase activity and metabolic quotient were determined and correlated with total and extractable metal concentrations in soil. The goal was to analyze complex interactions between toxic metals and microbial parameters by assessing the effect of soil organic carbon in the relationships. The effect of SOC was significant in all interactions and changed the correlations between microbial parameters and metal fractions from negative to positive. In some cases, the effect of SOC was combined with that of clay and soil pH. In the final analysis, dehydrogenase activity was negatively correlated to total metal concentrations and acetic acid extractable metals, respiration and metabolic quotient were to ammonium nitrate extractable metals. Dehydrogenase activity was the most sensitive microbial parameter correlating most frequently with contamination. Total and extractable zinc was most often correlated with microbial parameters. The large data set enabled robust explanation of discrepancies in organic matter functioning occurring frequently in analyzing of contaminated soil processes.

  3. Age-Related Reference Intervals of the Main Biochemical and Hematological Parameters in C57BL/6J, 129SV/EV and C3H/HeJ Mouse Strains.

    Directory of Open Access Journals (Sweden)

    Cristina Mazzaccara

    Full Text Available BACKGROUND: Although the mouse is the animal model most widely used to study the pathogenesis and treatment of human diseases, reference values for biochemical parameters are scanty or lacking for the most frequently used strains. We therefore evaluated these parameters in the C57BL/6J, 129SV/EV and C3H/HeJ mice. METHODOLOGY/PRINCIPAL FINDINGS: We measured by dry chemistry 26 analytes relative to electrolyte balance, lipoprotein metabolism, and muscle/heart, liver, kidney and pancreas functions, and by automated blood counter 5 hematological parameters in 30 animals (15 male and 15 female of each mouse strain at three age ranges: 1-2 months, 3-8 months and 9-12 months. Whole blood was collected from the retro-orbital sinus. We used quality control procedures to investigate analytical imprecision and inaccuracy. Reference values were calculated by non parametric methods (median and 2.5(th and 97.5(th percentiles. The Mann-Whitney and Kruskal-Wallis tests were used for between-group comparisons. Median levels of GLU, LDH, Chol and BUN were higher, and LPS, AST, ALP and CHE were lower in males than in females (p range: 0.05-0.001. Inter-strain differences were observed for: (1 GLU, t-Bil, K+, Ca++, PO(4- (p<0.05 and for TAG, Chol, AST, Fe++ (p<0.001 in 4-8 month-old animals; (2 for CK, Crea, Mg++, Na++, K+, Cl- (p<0.05 and BUN (p<0.001 in 2- and in 10-12 month-old mice; and (3 for WBC, RBC, HGB, HCT and PLT (p<0.05 during the 1 year life span. CONCLUSION/SIGNIFICANCE: Our results indicate that metabolic variations in C57BL/6J, 129SV/EV and C3H/HeJ mice after therapeutic intervention should be evaluated against gender- and age-dependent reference intervals.

  4. METABOLIC PARAMETERS CONCENTRATIONS IN BLOOD SERUM OF CZECH PIED BULLS DEPENDING ON SINGLE NUCLEOTIDE POLYMORPHISM OF LEPTIN GENE

    Directory of Open Access Journals (Sweden)

    Aleš Pavlík

    2014-02-01

    Full Text Available The aim of present study was to test hypothesis, that the leptin gene single nucleotide polymorphism (C/T giving missense mutation (Arg25Cys has an effect on concentration of blood serum total cholesterol, beta-hydroxybutyrate and urea in cattle. The experiment were performed in 58 Czech Pied bulls at 240 ± 9 days of age, which were divided in three experimental groups depending on different leptin genotypes (CC, n=28; CT, n=21; TT, n=9. Resulting genotypes in the exon 2 were CC (48.3%, CT (36.2%, and TT (15.5%. There were no differences in serum total cholesterol, urea, beta-hydroxybutyrate concentrations among the genotypes. Based on our results we may assume that analysed SNP of leptin gene have no effect on nutritional status and energy balance in fattened cattle.

  5. The Effect of Apium graveolens hydroalcoholic Seed Extract on Sperm Parameters and Serum Testosterone Concentration in Mice

    Directory of Open Access Journals (Sweden)

    P Kerishchi Khiabani

    2014-10-01

    Full Text Available Backgrounds & aim: apium graveolens contains antioxidant activity and high level of polyphenolics. The purpose of this study was to determaine the effect of Apium graveolens seeds extract on semen parameters and serum testosterone level in mice. Methods: In the present experimental study, sixty male mice were divided into three experimental groups and a control group. The hydroalcoholic seed extract of Apium graveolenas L. was administered intraperitoneally at the doses of 200, 400 and 800 mg/kg for 14 days. A week after the final injection, blood samples were collected for hormonal assay. Then, the testes weight, sperm count and cauda epididymal sperm motility was assessed. Data were analyzed by ANOVA and Tukey's test. Results: The results were compared with the control group indicating a significant increase in the total number of sperm at dose 400 mg.kg and increase sperm motility was seen in groups receiving 200 and 400 mg.kg respectively (P<0.001. Increased testosterone levels in the group receiving 400 mg.kg compared with the control group was observed (P<0.01. A significant increase was seen in testes weight compared with the control group (P<0.05. Conclusion: Apium graveolens seed extract appeared to be effective in improving semen parameters and serum total testosterones were dose dependent.

  6. 三种复方中草药对斑点叉尾鮰部分生化指标的影响%Effects of Three Compound Chinese Herbal Medicines on Some Biochemical Parameters in Ictalurus punctatus

    Institute of Scientific and Technical Information of China (English)

    郭永军; 乔之怡; 胡玉花; 陈成勋; 白东清; 丁莉莉; 刘志伟; 邢克智

    2012-01-01

    In order to test the effects of compound Chinese herbal medicine "Yuhuan", "Gantaile" and "Sanhuangfen" on biochemical parameters in Ictalurus punctatus, an oral administration trial was performed. The medicines mentioned above were added to a commercial feed at 5 g/kg separately, and were administrated to the tested fish. The fish was sampled after a continuous 7-day feeding. The biochemical parameters in blood and tissues were assayed. The results show that all the medicines enhanced SOD/MDA and CAT/MDA ratio in serum and spleen. So the antioxidant capacity in serum and spleen was enhanced. All the medicines reduced the antioxidant capacity in head kidney by means of reducing SOD/MDA and CAT/MDA ratio. The "Gantaile" and "Yuhuan" enhanced the antioxidant capacity in gill, while "Sanhuangfen" and "Yuhuan" enhanced the antioxidant capacity in muscle. All the medicines reduced AST and ALT activities in hepato-pancreas. The LZM activity was significantly cut down in hepato-pancreas by "Gantaile" and in head kidney by "Yuhuan", while "Sanhuangfen" boosted LZM activity in muscle significantly. All the medicines enhanced blood leucocytes phagocytosis and reduced AST and ALT activities in hepato-pancreas.%为研究复方中草药“鱼欢”、“肝泰乐”、“三黄粉”对斑点叉尾鮰生化指标的影响,将以上3种中草药按5g/kg饵料的比例加到饵料中,连续喂食斑点叉尾鮰7d后取样,测定血液和组织中的生化指标。结果表明:3种复方中草药均能提高血清和脾脏的SOD/MDA和cAz孙佃A比值,提高血清和脾脏的抗氧化能力;3种复方中草药均能降低头肾的SOD/MDA和CAT/MDA比值,降低其抗氧化能力。“肝泰乐”和“鱼欢”能提高鮰的抗氧化能力,“三黄粉”和“鱼欢”能提高肌肉的抗氧化能力。3种复方中草药能降低肝胰脏中AST和ALT的活力。“肝泰乐”显著降低肝胰脏的LZM活力,“鱼欢”显著降低头肾的LZM活力

  7. 明胶微球阻塞胎羊胎盘后血气及生化指标的变化%Alteration of Fetal Blood-Gas, pH and Biochemical Parameters after Placental Occlusion in Ewe

    Institute of Scientific and Technical Information of China (English)

    赵耐青

    2000-01-01

    目的 建立胎儿窘迫的妊娠羊动物模型并观测胎羊窘迫的动态过程。方法 妊娠晚期绵羊(湖羊)16头,于妊娠116~125d进宫腔行胎儿手术,胎羊股动脉插管,使导管顶端达胎羊腹主动脉水平,留置导管于胎羊体内,经三通管接压力感受器,连续动态监测胎羊血压,定期采血。手术成功后经胎羊腹主动脉注射明胶微球颗粒,阻塞胎盘建立胎儿窘迫的动物模型。每次注射微球前后胎羊采血作血气分析,留置血清检测血乳酸、高密度脂蛋白(HDL)和血糖等。结果 16例中10例手术成功,经胎羊腹主动脉注射微球后,pH、p∞2、和Po2,与微球注射的次数(MS)均有明显的关系,相关系数分别为-0.724 0(P0.05)和0.141 2(P>0.05)。乳酸浓度与pH值之间有明显的相关性,pH值7.0以下时,乳酸浓度明显升高。结论 经胎羊腹主动脉注射微球的方法,可以定量地表达胎盘阻塞的程度,符合胎儿窘迫的临床表现;该模型可动态地观察胎儿窘迫的过程。%Purpose To establish an animal model of fetal distress and to evaluate the alteration of pH, blood gas and biochemical parameters at fetal artery blood in ewe. Methods Intrauterine surgeries were performed at 116 - 125 gestational days in 16 ewes for vascular catheterization at fetal abdominal aorta 0.5 ml of 1% microspheres suspension was injected per 15 to 20 minutes after the surgeries had been succeed. Fetal blood pressure was continually monitored, and the fetal blood were sampled through the catheter before and after injection of microsphere for measurement of pH,blood gas,lactic acid,and high density lipoprotein (HDL). Results The of 16 pregnant sheeps were successfully operated. The times of microsphere injection (MS) was significantly related to fetal blood pH ( - 0. 724 0,P<0.01), Po2 (0. 521 5 ,P<0.05), p∞2( - 0. 700 79, P < 0.01 ) and lactate acid ( r = 0.50, P < 0.05 ). There was

  8. Quantification of Kinetic Rate Law Parameters of Uranium Release from Sodium Autunite as a Function of Aqueous Bicarbonate Concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Gudavalli, Ravi; Katsenovich, Yelena; Wellman, Dawn M.; Lagos, Leonel; Tansel, Berrin

    2013-09-05

    ABSTRACT: Hydrogen carbonate is one of the most significant components within the uranium geochemical cycle. In aqueous solutions, hydrogen carbonate forms strong complexes with uranium. As such, aqueous bicarbonate may significantly increase the rate of uranium release from uranium minerals. Quantifying the relationship of aqueous hydrogen carbonate solutions to the rate of uranium release during dissolution is critical to understanding the long-term fate of uranium within the environment. Single-pass flow-through (SPTF) experiments were conducted to estimate the rate of uranium release from Na meta-autunite as a function of bicarbonate solutions (0.0005-0.003 M) under the pH range of 6-11 and temperatures of 5-60oC. Consistent with the results of previous investigation, the rate of uranium release from sodium autunite exhibited minimal dependency on temperature; but were strongly dependent on pH and increasing concentrations of bicarbonate solutions. Most notably at pH 7, the rate of uranium release exhibited 370 fold increases relative to the rate of uranium release in the absence of bicarbonate. However, the effect of increasing concentrations of bicarbonate solutions on the release of uranium was significantly less under higher pH conditions. It is postulated that at high pH values, surface sites are saturated with carbonate, thus the addition of more bicarbonate would have less effect on uranium release. Results indicate the activation energies were unaffected by temperature and bicarbonate concentration variations, but were strongly dependent on pH conditions. As pH increased from 6 to 11, activation energy values were observed to decrease from 29.94 kJ mol-1 to 13.07 kJ mol-1. The calculated activation energies suggest a surface controlled dissolution mechanism.

  9. Investigating how fundamental parameters of XRF sample preparation and analysis affect the observed elemental concentration: an experiment using fluvial sediment from Sabah, Borneo.

    Science.gov (United States)

    Higton, Sam; Walsh, Rory

    2015-04-01

    X-Ray Fluorescence (XRF) is an important technique for measuring the concentrations of geochemical elements and inorganic contaminants adsorbed to sediments as an input to sediment tracing methods used to evaluate sediment transport dynamics in river catchments. In addition to traditional laboratory-based XRF instruments, the advent of increasingly advanced portable handheld XRF devices now mean that samples of fluvial sediment can be analysed in the field or in the laboratory following appropriate sample preparation procedures. There are limitations and sources of error associated with XRF sample preparation and analysis, however. It is therefore important to understand how fundamental parameters involved in sample preparation and analysis, such as sample compression and measurement exposure duration, affect observed variability in measurement results. Such considerations become important if the resulting measurement variability is high relative to the natural variability in element concentrations at a sample site. This paper deployed a simple experimental design to assess the impacts of varying a number of sample preparation and XRF analysis parameters on recorded measurements of elemental concentrations of the fine fraction (weight and sample quantity was sufficient to satisfy the assumption of 'infinite thickness' of sample. Standard plastic sample cups were used for both the Rigaku laboratory machine and the Niton portable XRF machine. A computer-controlled desktop laboratory stand was used in conjunction with the Niton handheld XRF analyser to ensure consistent repeated measurements. Parameters investigated related to sample preparation included consistent mechanical compression of samples within the sample cup and film thickness. Parameters investigated related to XRF analysis included the XRF machine selected and measurement exposure duration. As XRF is a non-destructive technique, wherever possible the same sample material was used to test different

  10. Ethanol extract of mango (Mangifera indica L.) peel inhibits α-amylase and α-glucosidase activities, and ameliorates diabetes related biochemical parameters in streptozotocin (STZ)-induced diabetic rats.

    Science.gov (United States)

    Gondi, Mahendranath; Prasada Rao, U J S

    2015-12-01

    Peel is a major by-product during processing of mango fruit into pulp. Recent report indicates that the whole peel powder ameliorated diabetes. In the present study, ethanolic extract of mango peel was analysed for its bioactive compounds, evaluated for α-amylase and α-glucosidase inhibitory properties, oral glucose tolerance test, antioxidant properties, plasma insulin level and biochemical parameters related to diabetes. In addition to gallic and protocatechuic acids, the extract also had chlorogenic and ferulic acids, which were not reported earlier in mango peel extracts. The peel extract inhibited α-amylase and α-glucosidase activities, with IC50 values of 4.0 and 3.5 μg/ml. Ethanolic extract of peel showed better glucose utilization in oral glucose tolerance test. Treatment of streptozotocin-induced diabetic rats with the extract decreased fasting blood glucose, fructosamine and glycated hemoglobin levels, and increased plasma insulin level. Peel extract treatment decreased malondialdehyde level, but increased the activities of antioxidant enzymes significantly in liver and kidney compared to diabetic rats. These beneficial effects were comparable to metformin, but better than gallic acid treated diabetic rats. The beneficial effects of peel extract may be through different mechanism like increased plasma insulin levels, decreased oxidative stress and inhibition of carbohydrate hydrolyzing enzyme activities by its bioactive compounds. Thus, results suggest that the peel extract can be a potential source of nutraceutical or can be used in functional foods and this is the first report on antidiabetic properties of mango peel extract.

  11. Application of the MOS-C-V technique to determine impurity concentrations and surface parameters on the diffused face of silicon solar cells

    Science.gov (United States)

    Weinberg, I.

    1975-01-01

    The feasibility of using the MOS C-V technique to obtain information regarding impurity and surface state concentrations on the diffused face of silicon solar cells with Ta2O5 coatings is studied. Results indicate that the MOS C-V technique yields useful information concerning surface parameters which contribute to the high, efficiency limiting, surface recombination velocities on the n+ surface of silicon solar cells.

  12. Analysis of the routine blood counts and blood biochemical parameters and hemorheology in the rats with congenital cataract%先天性白内障大鼠血液生化及血液流变学分析

    Institute of Scientific and Technical Information of China (English)

    田小芸; 尤金炜; 董敏; 许龙祥; 赵志刚; 陈莉; 恽时锋

    2014-01-01

    Objective To measure the range of routine blood counts and blood biochemical parameters ,to analyze hemorheology of the rats with congenital cataract .Methods Blood samples were taken from 90 rats with congenital cataract weight about 185 ~211 g.Routine blood analysis was performed and blood biochemical and hemorheology paramenters were determind using an automatic blood biochemical and hemorheology analyzer .Results There were no significant difference ( P >0.05 ) between the cataract rats and the normal rats in Blood test results; but there were significant difference between the microphthalmos cataract rats and the normal same -sex rats ( P <0.01 or P <0.05 ) . The biochemical results is the cataract rats and the normal rats were different significantly in ALB group ( P <0.01 or P<0.05), and the female microphthalmos cataract rats compared with the control rats had significant difference in Ure (P<0.01) , the female cataract rats ompared with the normal rats were very significant difference in Cr group ( P <0.05 or P <0.01).The erythrocyte counts of the male cataract rats and male microphthalmos cataract rats were significantly lower than that in the female ones, respectively(P <0.05, P <0.01).The platelet counts of the male cataract rats and the male microphthalmos cataract rats were significantly higher than that in the female ones , respectively(P <0.01), and the creatinine of the male cataract rats and the male microphthalmos cataract rats were significantly lower then that in the female ones, respectively(P <0.01).There were no significant difference in every group on hemorheology .Conclusions There were significant differences in some blood indexes between the congenital cataract rats and the normal rats .These data may become useful reference for biomedical researcher in this field .%目的:测定先天性白内障大鼠血液常规、生化正常值及血液流变学变化。方法采用XS-800i血常规分析仪和OLYMPUS AV2700

  13. Factors influencing particle number concentrations, size distributions and modal parameters at a roof-level and roadside site in Leicester, UK.

    Science.gov (United States)

    Agus, Emily L; Young, David T; Lingard, Justin J N; Smalley, Robert J; Tate, James E; Goodman, Paul S; Tomlin, Alison S

    2007-11-01

    Measurements of urban particle number concentrations and size distributions in the range 5-1000 nm were taken at elevated (roof-level) and roadside sampling sites on Narborough Road in Leicester, UK, along with simultaneous measurements of traffic, NO(x), CO and 1,3-butadiene concentrations and meteorological parameters. A fitting program was used to determine the characteristics of up to five modal groups present in the particle size distributions. All particle modal concentrations peaked during the morning and evening rush hours. Additional events associated with the smallest mode, that were not observed to be connected to primary emissions, were also present suggesting that this mode consisted of newly formed secondary particles. These events included peaks in concentration which coincided with peaks in solar radiation, and lower concentrations of the larger modes. Investigation into the relationships between traffic flow and occupancy indicated three flow regimes; free-flow, unstable and congested. During free-flow conditions, positive linear relationships existed between traffic flow and particle modal number concentrations. However, during unstable and congested periods, this relationship was shown to break-down. Similar trends were observed for concentrations of the gas phase pollutants NO(x), CO and 1,3-butadiene. Strong linear relationships existed between NO(x), CO, 1,3-butadiene concentrations, nucleation and Aitken mode concentrations at both sampling locations, indicating a local traffic related emission source. At the roadside, both nucleation and Aitken mode are best represented by a decreasing exponential function with wind speed, whereas at the roof-level this relationship only occurred for Aitken mode particles. The differing relationships at the two sampling locations are most likely due to a combination of meteorological factors and distance from the local emission source.

  14. Impacts of the solar eclipse of 29 March 2006 on the surface ozone concentration, the solar ultraviolet radiation and the meteorological parameters at Athens, Greece

    Directory of Open Access Journals (Sweden)

    C. Tzanis

    2008-01-01

    Full Text Available In this study the variations in the surface ozone concentration, the solar ultraviolet radiation and the meteorological parameters at the ground before, during and after the total solar eclipse of 29 March 2006 have been examined. This analysis is based on the measurements performed at four stations located in the greater Athens basin in Greece. The experimental data demonstrated that the solar eclipse phenomenon affects the surface ozone concentration as well as the temperature, the relative humidity and the wind speed near the ground. The decrease in the surface ozone concentration that observed after the beginning of the eclipse event lasted almost two hours, probably due to the decreased efficiency of the photochemical ozone formation. The reduction of the solar ultraviolet radiation at 312 and 365 nm reached 97% and 93% respectively, while the air temperature dropped, the relative humidity increased and the wind speed decreased.

  15. Influences of Technological Parameters on Smelting-separation Process for Metallized Pellets of Vanadium-bearing Titanomagnetite Concentrates

    Institute of Scientific and Technical Information of China (English)

    En-hui WU; Rong ZHU; Shao-li YANG; Lan MA; Jun LI; Jing HOU

    2016-01-01

    The smelting-separation process for metallized pellets of vanadium-bearing titanomagnetite concentrates was studied.The influences of smelting temperature,smelting time,and the basicity of the metallized pellet on vana-dium and iron recovery were investigated.The characteristics of titanium slag were analyzed using X-ray diffraction, energy dispersive spectroscopy,and mineralographic microscopic analysis.The results demonstrate that appropriate increases in smelting temperature and smelting time can improve the vanadium and iron recovery from metallized pel-lets and are beneficial for the slag-iron separation.Although increasing the basicity of the metallized pellet can consid-erably improve the vanadium and iron recovery,the TiO2 grade of titanium slag was decreased.Under the optimal conditions,90·17% of vanadium and 92·98% of iron in the metallized pellet were recovered,and the TiO2 grade of titanium slag was 55·01%.It was found that anosovite,augite,spinel,glassiness,and metallic iron were the main mineral phases of the titanium slag.

  16. Associative learning in biochemical networks

    OpenAIRE

    Ghandi, Nikhil; Ashkenasy, Gonen; Tannenbaum, Emmanuel

    2007-01-01

    We develop a simple, chemostat-based model illustrating how a process analogous to associative learning can occur in a biochemical network. Associative learning is a form of learning whereby a system "learns" to associate two stimuli with one another. In our model, two types of replicating molecules, denoted A and B, are present in some initial concentration in the chemostat. Molecules A and B are stimulated to replicate by some growth factors, denoted GA and GB, respectively. It is also assu...

  17. Molecular and biochemical studies on bovine ephemeral fever

    Directory of Open Access Journals (Sweden)

    Nahed S. Thabet; Emad W. Ghazy; Mohamed A. Nayel; Mohamed Abo-Elkhair

    2011-05-01

    Full Text Available Bovine ephemeral fever (BEF in cattle has been reported to be associated with a range of biochemical changes which are similar to those seen in milk fever. This study aimed to clarify the biochemical alterations that associate infection of cattle with BEF with special references to the mechanisms involved in the development of hypocalcemia. The study was conducted on 30 cases of cattle infected with BEF based on the characteristic clinical signs which were confirmed by isolation of virus and RT-PCR. Another 6 healthy cows were used in the study as control. The evaluated parameters included biochemical variables such as serum values of total protein (TP, albumin (Alb, glucose (Glu, total calcium (tCa, ionized calcium (iCa, inorganic phosphorus (P, magnesium (Mg, sodium (Na, potassium (K, chloride (Cl, creatinine (Cr, blood urea nitrogen (BUN and serum activity of alkaline phosphatase (ALP. Hormonal profile included parathyroid hormone (PTH, insulin (Ins, and cortisol (Cor. The results showed that BEF-infected animals demonstrated a significant decrease (P<0.05 in serum concentrations of TP, Glo, iCa, P, Na, K, BUN and ALP while the mean values of serum levels of Glu and Cl were significantly increased (P<0.05. The mean values of serum levels of PTH were significantly decreased (P<0.05 while serum concentrations of Ins and Cor showed a significant increase. It was concluded that the clinical signs of bovine ephemeral fever are related to the hypocalcemia resulting from suppression of parathyroid hormone which seems to be mediated by respiratory alkalosis caused by the disease. This explanation needs future studies to provide a direct link between measurement of blood indicators of acid-base status, blood biochemical parameters and urine analysis. However, this work can provide a good knowledge about the pathogenesis of the disease that can lead to better management and proper treatment.

  18. A new method to estimate photosynthetic parameters through net assimilation rate-intercellular space CO2 concentration (A-Ci ) curve and chlorophyll fluorescence measurements.

    Science.gov (United States)

    Moualeu-Ngangue, Dany P; Chen, Tsu-Wei; Stützel, Hartmut

    2017-02-01

    Gas exchange (GE) and chlorophyll fluorescence (CF) measurements are widely used to noninvasively study photosynthetic parameters, for example the rates of maximum Rubisco carboxylation (Vcmax ), electron transport rate (J), daytime respiration (Rd ) and mesophyll conductance (gm ). Existing methods for fitting GE data (net assimilation rate-intercellular space CO2 concentration (A-Ci ) curve) are based on two assumptions: gm is unvaried with CO2 concentration in the intercellular space (Ci ); and light absorption (α) and the proportion of quanta absorbed by photosystem II (β) are constant in the data set. These may result in significant bias in estimating photosynthetic parameters. To avoid the above-mentioned hypotheses, we present a new method for fitting A-Ci curves and CF data simultaneously. This method was applied to a data set obtained from cucumber (Cucumis sativus) leaves of various leaf ages and grown under eight different light conditions. The new method had significantly lower root mean square error and a lower rate of failures compared with previously published methods (6.72% versus 24.1%, respectively) and the effect of light conditions on Vcmax and J was better observed. Furthermore, the new method allows the estimation of a new parameter, the fraction of incoming irradiance harvested by photosystem II, and the dependence of gm on Ci .

  19. 苯致白血病患者外周血细胞和生化指标的分析%Analysis of peripheral blood cells and biochemical parameters of benzene-induced leukemia

    Institute of Scientific and Technical Information of China (English)

    杨祥丽; 王佃鹏; 周飞; 张艳芳; 李培茂; 张志敏; 邓丽丹; 罗斌

    2016-01-01

    目的:分析职业性苯致慢性粒细胞白血病外周血象及肝功能、肾功能、血糖、血脂参数变化规律。方法选取61例健康体检者作为对照组,10例苯致慢性粒细胞白血病患者作为病例组,对两组研究对象进行外周血细胞计数分析和血清生化(肝功能、肾功能、葡萄糖、血脂)参数检测分析。结果病例组的红细胞、血红蛋白、红细胞压积、红细胞分布宽度、平均红细胞体积、白细胞、中性粒细胞绝对值、淋巴细胞绝对值、嗜碱性细胞绝对值、血小板比积、大血小板比率、总蛋白、清蛋白、球蛋白、总胆红素、直接胆红素、间接胆红素、葡萄糖、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、尿酸和对照组比较,差异有统计学意义(P<0.05)。结论苯致慢性粒细胞白血病患者血细胞参数异常,肝肾功能、血脂及存在异常,定期监测这些指标具有重要意义。%Objective To investigate the liver function ,kidney function ,glucose ,lipids and blood cell sparameter changes of ben‐zene‐induced chronic myeloid leukemia cases in peripheral blood .Methods 61 normal healthy volunteers as the control groups ,10 benzene‐induced chronic myeloid leukemia patients as the case groups .Peripheral blood cell were counted and serum biochemical pa‐rameter(liver function ,kidney function ,glucose ,blood lipids) wer detected .Results The parameters including of RBC ,hemoglobin , hematocrit ,red blood cell distribution width ,mean corpuscular volume ,leukocytes ,absolute neutrophil count ,absolute lymphocyte , basophils ,platelet hematocrit ,large platelet ratio ,total protein ,albumin ,globulin ,total bilirubin ,direct bilirubin ,indirect bilirubin , alkaline phosphatase ,total cholesterol ,high density lipoprotein cholesterol ,low density lipoprotein cholesterol ,creatinine ,uric acid , creatine kinase ,lactate dehydrogenase ,

  20. Effects of heavy metal (Pb) concentration on some growth parameters of plants grown in lead polluted soil under organic fertilizer amendment

    Institute of Scientific and Technical Information of China (English)

    Ojo M Oseni; Adekunle A Adelusi; Esther O Dada; Abdulfatai B Rufai

    2016-01-01

    This study investigated morphological variation and biomass accumulation that occurred inSida acuta andChromolaena odorata plants grown in lead polluted soil under organic fertilizer amendment. The study was carried out in the screen house at the Biological Gardens of the Obafemi Awolowo University, Ile-Ife, Osun State. The experiment was a factorial combination of one heavy metal (Pb) at five levels of concentration (0, 200, 400, 800 and 1,000 mg/kg) in a completely randomized design, and were replicated three times for each of the two plants and two levels (0 g/kg and 9.4 g/kg) of organic fertilizer (OBD-Plus). Each pot was filled with 5 kg of air-dried and sieved soil and placed on a plastic tray for the collection of excess water. Two weeks after planting, seedlings of uniform height were transplanted from the nursery to experimental pots at the rate of one seedling per pot and grown for 10 weeks. The growth parameters of the plants were biomonitored for 7 weeks. After 10 weeks of treatment, the plants were harvested and dried to calculate the biomass accumulation. The two plant species performed better under fertilizer application than without it. For each of the plant species the growth parameters decreased as the levels of Pb concentration increased. Furthermore, the plants' biomass decreased significantly (p<0.05) as the levels of Pb concentration increased. The organic fertilizer helped to improve the plants' performance in lead-polluted soil.

  1. Feed intake, digestibility, body weight and carcass parameters of Afar rams fed tef (Eragrostis tef) straw supplemented with graded levels of concentrate mix.

    Science.gov (United States)

    Hagos, Tesfay; Melaku, Solomon

    2009-04-01

    The experiment was conducted at Alamata Agricultural Research Center, Ethiopia using 20 Afar rams with an initial body weight (BW) of 18.2 +/- 1.76 (mean +/- SD) kg. The objectives were to study the effect of supplementation with concentrate mix consisting of wheat bran (WB), noug seed cake (NSC) and sesame seed cake (SSC) at the ratio of 2:1:1 on dry matter (DM) basis, respectively on feed intake, digestibility, BW gain and carcass parameters of Afar rams fed tef (Eragrostis tef) straw basal diet. The experiment was arranged with four treatments and five replications in a randomized complete block design. The treatments included feeding sole tef straw (T1, control), and daily supplementation with the concentrate mix offered at 150 (T2, low), 250 (T3, medium) and 350 (T4, high) g DM per head. Total DM intake, crude protein (CP) digestibility, daily BW gain (P mix supplementation. Moreover, the medium level of supplementation did not substitute tef straw intake. Therefore, it is concluded that the medium level of concentrate mix supplement maintained the utilization of the roughage feed and resulted in better carcass parameters.

  2. The hydrogen concentration as parameter to identify natural attenuation processes of volatile chlorinated hydrocarbons in ground water; Die Wasserstoffkonzentration als Parameter zur Identifizierung des natuerlichen Abbaus von leichtfluechtigen Chlorkohlenwasserstoffen (LCKW) im Grundwasser

    Energy Technology Data Exchange (ETDEWEB)

    Alter, M.D.

    2006-06-15

    In this study, the hydrogen concentration as parameter to identify natural attenuation processes of volatile chlorinated hydrocarbons was investigated. The currently accepted and recommended bubble strip method for hydrogen sampling was optimized, and a storage method for hydrogen samples was developed. Furthermore batch experiments with a dechlorinating mixed culture and pure cultures were carried out to study H{sub 2}-concentrations of competing redox processes. The extraction of hydrogen from ground water was optimized by a reduced inlet diameter of the usually applied gas sampling bulbs, allowing a maximal turbulent ow and gas transfer. With a gas volume of 10 ml and flow rates of 50 to 140 ml/min, the course of extraction almost followed the theoretical course of equilibration. At flow rates > 100 ml/min a equilibrium of 98% was achieved within 20 min. Until recently it was generally accepted that hydrogen samples can be stored only for 2 hours and therefore have to be analyzed immediately in the eld. Here, it was shown that eld samples can be stored for 1-3 days until analysis. For the dechlorination of tetrachloroethene (PCE), a hydrogen threshold concentration of 1-2 nM was found with the dechlorinating mixed culture as well as with a pure culture of Sulfurospirillum multivorans in combination with another pure culture Methanosarcina mazei. No dechlorination was detectable below this concentration. With the dechlorinating mixed culture, this finding is valid for all successive dechlorination steps until ethene. The hydrogen threshold concentration for denitrification were below the detection limit of 0,2 nM with the dechlorinating mixed culture. A threshold concentration of 3,1-3,5 nM was found for sulphate reduction and a threshold of 7-9 nM H{sub 2} for hydrogenotrophic methanogenesis. This implies that the natural dechlorination at contaminated sites is preferred to competing processes like sulphate reduction and methanogenesis. The threshold

  3. 面向植物生理生化参数反演的光谱信息压缩感知重构%Compressive sensing and reconstruction of vegetation spectra for retrieving plant physiological and biochemical parameters

    Institute of Scientific and Technical Information of China (English)

    徐平; 刘俊峰; 张竞成; 薛凌云

    2016-01-01

    With the development of hyperspectral technology, it is of great significance to establish a specific compression and reconstruction method that can be used for conducting quantitative remote sensing of vegetation. Such a method is expected not only to improve the efficiency of data storage and transmission, but also to maintain the main spectral characteristics in interpreting some physiological and biochemical parameters. In this study, the compressive sensing technique was introduced to the compression and reconstruction of plant spectrum. Three critical physiological and biochemical parameters of plant, i.e. water content, carotenoid content and chlorophyll content, were chosen to test the retrieving efficacy of the proposed method. To facilitate such an analysis, a spectral dataset consisting of 2 500 spectra and corresponding plant physiological and biochemical parameters with multivariate normal distribution was generated by a classic plant leaf radiation model PROSEPCT. Based on the data, the compression and reconstruction method that was specific for vegetation spectral processing was proposed, described and evaluated. To process the spectral dataset using the compressed sensing method, the spectral dataset was firstly sampled by means of constructing random matrix. Then, the sampled data were reconstructed by a classic orthogonal matching pursuit algorithm, and the normalized root mean square error between the reconstructed data and the original data was analyzed. The performance of the method was thoroughly evaluated on 3 different levels: the spectral level, the feature level and the model level. At the spectral level, an error analysis was performed by directly calculating the original spectra and reconstructed spectra of corresponding samples. At the spectral index level, the spectral index was calculated based on both the original spectra and the reconstructed spectra. Then the error of the spectral index was analyzed. At the model level, the

  4. Effects of conjugated linoleic acids and dietary concentrate proportion on performance, milk composition, milk yield and metabolic parameters of periparturient dairy cows.

    Science.gov (United States)

    Petzold, Maria; Meyer, Ulrich; Kersten, Susanne; Spilke, Joachim; Kramer, Ronny; Jahreis, Gerhard; Dänicke, Sven

    2013-06-01

    The study aimed to examine effects of supplemented conjugated linoleic acids (CLA) to periparturient cows receiving different concentrate proportions ante partum (a.p.) to investigate CLA effects on lipid mobilisation and metabolism. Compared to adapted feeding, a high-concentrate diet a.p. should induce a ketogenic metabolic situation post partum (p.p.) to better understand how CLA works. Sixty-four pregnant German Holstein cows had ad libitum access to partial mixed rations 3 weeks prior to calving until day 60 p.p. Ante partum, cows received control fat (CON) or a CLA supplement at 100 g/d, either in a low-concentrate (CON-20, CLA-20) or high-concentrate diet (CON-60, CLA-60). Post partum, concentrate proportion was adjusted, while fat supplementation continued. After day 32 p.p., half of the animals of CLA-groups changed to CON supplementation (CLA-20-CON, CLA-60-CON). A ketogenic metabolic situation p.p. was not achieved and therefore impacts of CLA could not be examined. Live weight, milk yield and composition, blood parameters remained unaffected by the treatments. Only a slightly reduced milk fat yield (not significant) was recorded for Group CLA-20. The proportion of trans-10,cis-12 (t10,c12) CLA in milk fat was significantly increased in CLA-groups compared to CON-groups. With the exception of a reversible CLA effect on milk fat in Group CLA-20, no post-treatment effects occurred. Dry matter intake (DMI) of Group CLA-60 was highest before calving, resulting in a significantly improved estimated energy balance after calving. Ante partum, net energy intakes were significantly increased in high-concentrate groups. Overall, supplemented CLA preparation did not relieve metabolism and lipid mobilisation of early lactating cows. But feeding CLA in a high-concentrate diet a.p. seems to increase DMI and thereby improve the energy balance of cows immediately after calving.

  5. Iron Concentration-Induced Changes in Growth and Biochemical Compositions of Marine Diatom Phaeodactylum tricornutum(Bacillariophyceae)%铁浓度诱导的三角褐指藻生长和生化组分变化

    Institute of Scientific and Technical Information of China (English)

    蔡卓平; 黄伟伟; 段舜山

    2008-01-01

    As long as microalgal growth is not overprolific in the water, marine microalgae are of great benefit to marine ecosystem because they not only form the basis of marine food web, but also consume the greenhouse gas carbon dioxide in the atmosphere during photosynthesis. Sometimes, however, red tides would occur due to the overproliferation or o