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Sample records for biochemical parameters compared

  1. The Comparative Analysis of Biochemical Parameters in Patients with Pleural Effusions: A Prospective Study

    Directory of Open Access Journals (Sweden)

    Ali Kutluk

    2016-09-01

    Full Text Available Aim: The differentiation of exudative from transudative effusion is important to lead the clinician in making further biochemical analysis for possible etiology and in choosing the appropriate treatment strategy. The aim of the study is to evaluate the diagnostic value of biochemical parameters together with Light%u2019s criteria to differentiate exudative from transudative effusions. Material and Method: The LDH, total protein, albumin, adenosine deaminase (ADA, apolipoprotein A1, apolipoprotein B, Lipoprotein-A, total cholesterol, HDL-cholesterol, VLDL-cholesterol, LDL-cholesterol, and triglyceride levels of patients with unknown etiology were measured both in plasma and pleural fluid. Mann-Whitney U was used to compare the groups and p < 0.05 was accepted as statistical significance. Sensitivity, specificity, and accuracy were calculated for each biochemical parameter. The ROC analysis was used to estimate the optimum cut-off value for the highest sensitivity and specificity.Results: Pleural LDH (p=0.001, total protein (p=0.001, albumin (p=0.001, triglyceride (p=0.001, total cholesterol (p=0.001, HDL-cholesterol (p=0.042, VLDL-cholesterol (p=0.001, LDL-cholesterol (p=0.001, apolipoprotein A1 (p=0.021, and HDL-cholesterol/LDL-cholesterol ratio (p=0.048 were found significant in differentiating exudative from transudative effusions. Discussion: The study showed that the use of pleural LDH, total cholesterol, and HDL-cholesterol levels together is more significant than Light%u2019s criteria. The sensitivity, specificity, and accuracy of this test were 99%, 94.1%, and 96.2% respectively.

  2. Comparative study; physiological and biochemical parameters of normal and induced dehydrated condition of rabbits

    International Nuclear Information System (INIS)

    Bashir, S.; Bukhari, I.

    2008-01-01

    Biochemical and physiological parameters like body weight, blood pH. Blood glucose, total lipids total protein, globulin, albumin and albumin/globulin ratio were determined in twelve rabbits each normal and after the induction of diseased condition i.e. dehydration. Statistically significant differences were identified when the comparison made between normal rabbits and their respective dehydrated group. Blood glucose total lipid packed cell. Volume and globulin increased significantly where where as body weight, albumin and albumin/globulin ratio decreased significantly. These differences in the physiological and biochemical parameters in disease induced condition require the necessity for analyzing this condition for the changes in the pharmacokinetics parameter like, absorption distribution metabolism and excretion leading to alteration in the pharmacokinetics of drug. (author)

  3. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    AJB SERVER

    2007-01-04

    Jan 4, 2007 ... The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and.

  4. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and basophiles counts were ...

  5. Comparative effects of mature coconut water (Cocos nucifera and glibenclamide on some biochemical parameters in alloxan induced diabetic rats

    Directory of Open Access Journals (Sweden)

    P. P. Preetha

    2013-03-01

    Full Text Available In the present study, comparative effects of mature coconut water (Cocos nucifera L., Arecaceae and glibenclamide in alloxan induced diabetic rats were evaluated. Diabetes mellitus was induced in Sprague-Dawly rats using alloxan monohydrate (150 mg kg-1 body weight. Treatment with lyophilized form of mature coconut water and glibenclamide in diabetic rats reduced the blood glucose and glycated hemoglobin along with improvement in plasma insulin level. Elevated levels of liver function enzymes markers like alkaline phosphatase, serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase in diabetic rats were significantly reduced on treatment with mature coconut water. In addition to this, diabetic rats showed altered levels of blood urea, serum creatinine, albumin, albumin/globulin ratio which were significantly improved by treatment with mature coconut water and glibenclamide. Activities of nitric oxide synthase in liver and plasma L-arginine were reduced significantly in alloxan induced diabetic rats while treatment with mature coconut water reversed these changes. The overall results show that mature coconut water has significant beneficial effects in diabetic rats and its effects were comparable to that of glibenclamide, a well known antidiabetic drug.

  6. Evaluation of Haematological and Biochemical Parameters of ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Evaluation of Haematological and Biochemical Parameters of Juvenile Oreochromis niloticus after Exposure to Water Soluble Fractions of ... niloticus were evaluated. After a preliminary determination of the 96 h-LC50 of ... evaporation, dissolution, emulsion, photolysis and biodegradation which generate a water soluble.

  7. Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice: Part II: Impact on specific chemical and biochemical quality parameters

    NARCIS (Netherlands)

    Vervoort, L.; Plancken, van der I.; Grauwet, T.; Timmermans, R.A.H.; Mastwijk, H.C.; Matser, A.M.; Hendrickx, M.E.; Loey, van A.

    2011-01-01

    The impact of thermal, high pressure (HP) and pulsed electric field (PEF) processing for mild pasteurization of orange juice was compared on a fair basis, using processing conditions leading to an equivalent degree of microbial inactivation. Examining the effect on specific chemical and biochemical

  8. Lipoprotein (a) and biochemical parameters in elderly

    OpenAIRE

    Yuttana Sudjaroen

    2016-01-01

    Background: Lipoprotein (a) [Lp(a)] is an low-density lipoprotein like particle and is an important independent risk factor for coronary artery diseases (CAD). Few studies on Lp(a) level in Thai elderly to screening risk of CAD may concerned. Aims: To study the relation of Lp(a) level and routine biochemical parameters including lipid profiles and fasting blood glucose in elderly and to determine risk of subclinical symptoms by using Lp(a) levels as early risk predictor. Settings and Design: ...

  9. Opium and heroin alter biochemical parameters of human's serum.

    Science.gov (United States)

    Kouros, Divsalar; Tahereh, Haghpanah; Mohammadreza, Afarinesh; Minoo, Mahmoudi Zarandi

    2010-05-01

    Iran is a significant consumer of opium, and, generally, of opioids, in the world. Addiction is one of the important issues of the 21st century and is an imperative issue in Iran. Long-term consumption of opioids affects homeostasis. To determine the effects of opium and heroin consumption on serum biochemical parameters. In a cross-sectional study, subjects who had consumed heroin (n = 35) or opium (n = 42) for more than two years and 35 nonaddict volunteers as the control group were compared in regard to various biochemical parameters such as fasting blood sugar (FBS), Na(+), K(+), Ca(2+), blood urea nitrogen (BUN), uric acid (UA), triglyceride (TG), cholesterol, creatinine, and total protein. Chromatography was used to confirm opioid consumption, and the concentration of biochemical parameters was determined by laboratory diagnostic tests on serum. No significant differences were found in Na(+), Ca(2+), BUN, UA, TG, creatinine, and total protein concentrations among the three groups. FBS, K(+), and UA levels were significantly lower in opium addicts compared to the control group. Serum Ca(2+) concentration of heroin addicts showed a significant decrease compared to that of the control group. Both addict groups showed a significant decrease in serum cholesterol levels. Chronic use of opium and heroin can change serum FBS, K(+), Ca(2+), UA, and cholesterol. This study, one of few on the effects of opium on serum biochemical parameters in human subjects, has the potential to contribute to the investigation of new approaches for further basic studies.

  10. Accelerated maximum likelihood parameter estimation for stochastic biochemical systems

    Directory of Open Access Journals (Sweden)

    Daigle Bernie J

    2012-05-01

    Full Text Available Abstract Background A prerequisite for the mechanistic simulation of a biochemical system is detailed knowledge of its kinetic parameters. Despite recent experimental advances, the estimation of unknown parameter values from observed data is still a bottleneck for obtaining accurate simulation results. Many methods exist for parameter estimation in deterministic biochemical systems; methods for discrete stochastic systems are less well developed. Given the probabilistic nature of stochastic biochemical models, a natural approach is to choose parameter values that maximize the probability of the observed data with respect to the unknown parameters, a.k.a. the maximum likelihood parameter estimates (MLEs. MLE computation for all but the simplest models requires the simulation of many system trajectories that are consistent with experimental data. For models with unknown parameters, this presents a computational challenge, as the generation of consistent trajectories can be an extremely rare occurrence. Results We have developed Monte Carlo Expectation-Maximization with Modified Cross-Entropy Method (MCEM2: an accelerated method for calculating MLEs that combines advances in rare event simulation with a computationally efficient version of the Monte Carlo expectation-maximization (MCEM algorithm. Our method requires no prior knowledge regarding parameter values, and it automatically provides a multivariate parameter uncertainty estimate. We applied the method to five stochastic systems of increasing complexity, progressing from an analytically tractable pure-birth model to a computationally demanding model of yeast-polarization. Our results demonstrate that MCEM2 substantially accelerates MLE computation on all tested models when compared to a stand-alone version of MCEM. Additionally, we show how our method identifies parameter values for certain classes of models more accurately than two recently proposed computationally efficient methods

  11. Biochemical parameters in chronic renal failure.

    Science.gov (United States)

    Hakim, R M; Lazarus, J M

    1988-03-01

    We analyzed biochemical data derived from 911 patients with renal insufficiency observed at our institution for periods up to 7 years. During early renal failure (RF) (creatinine less than 5 mg/dL), the rate of change of hematocrit, total CO2 (tCO2) and urea per unit change of creatinine was significantly higher than during moderate (creatinine between 5 and 10 mg/dL) or advanced (creatinine greater than 10 mg/dL) RF. For example, the rate of change of hematocrit (%, volume/volume [v/v]) was (mean +/- SEM) -2.15 +/- 0.15% for each 1 mg/dL increase in creatinine in the range of creatinine less than 5 mg/dL, whereas for the range of creatinine greater than 10 mg/dL, the rate of change was only -0.48 +/- 0.06% (P less than 0.001). Similarly, the rate of change of tCO2 was -1.68 +/- 0.09 mEq/L for each 1 mg/dL increment in creatinine concentration during early RF, and -0.19 +/- 0.09 mEq/L per unit increase in creatinine during advanced RF (P less than 0.001). Chloride concentration initially increased as a function of creatinine in early RF, but decreased in advanced RF, whereas the anion gap increased throughout the course of RF. Mean serum phosphate concentration also increased steadily, but remained below the upper range of normal (4.7 mg/dL) during early RF without the use of phosphate binders. These data suggest that different biochemical parameters change at different rates as a function of the severity of renal dysfunction, and that although phosphate retention may occur, hyperphosphatemia is not a hallmark of early RF.

  12. haematological parameters and serum biochemical indices of pre

    African Journals Online (AJOL)

    mrmrsolayiwola

    2012-05-01

    BWSFM) on haematological and serum biochemical parameters in rabbit were studied. Thirty-two (32) cross-bred. (New Zealand-white X Chinchilla) male weaner rabbits aged between 6 and 9 weeks were randomly.

  13. Diagnostic utility of clinical and biochemical parameters in ...

    African Journals Online (AJOL)

    Diagnostic utility of clinical and biochemical parameters in pancreatic head malignancy ... Department of Surgery, Sir Run Run Shaw Hospital College of Medicine, Zhejiang University, ..... technical review on the epidemiology, diagnosis, and.

  14. Reference Physiological Ranges for Serum Biochemical Parameters ...

    African Journals Online (AJOL)

    drugs includes measurement of changes in physiological parameters of subjects from known established baseline ... Methods: After informed consent, blood and urine samples were collected from a total of 576 ... a major public health problem in Cameroon with a .... sample collection, processing, storage and handling.

  15. Effect of genotype on haematology and biochemical parameters of ...

    African Journals Online (AJOL)

    Frizzle n = 33, Naked neck, n= 33 and Normal n = 33) were generated from 36 matured local chickens and used for the study to determine the effect of genotype on hematological and biochemical parameters of local chicken in the humid ...

  16. Haematological and serum biochemical parameters of West African ...

    African Journals Online (AJOL)

    The study was conducted to evaluate the haematological and serum biochemical parameters of West African dwarf goats fed ensiled cassava leaves with molasses and caged layer waste. Eighteen West African dwarf goats were randomly assigned to three experimental diets consisting of cassava leaves ensiled alone ...

  17. Haematological and Serum Bio-Chemical Parameters of West ...

    African Journals Online (AJOL)

    Haematological and Serum Bio-Chemical Parameters of West African Dwarf and Kalahari Red Goats in the Humid Tropics. ... Haematological results showed that white blood cell count, haemoglobin concentration, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin ...

  18. Complement Levels and Haemate-Biochemical Parameters as ...

    African Journals Online (AJOL)

    Complement levels and haemato-biochemical parameters in West African Dwarf (WAD) and Borno White (BW) goats experimentally infected with Trypanosoma congolense were investigated. Parasitaemia was established in both breeds of goats by day 7 post-infection. Peak parasitaemia of 7.5 x 103/µL for WAD goats was ...

  19. haematological parameters and serum biochemical indices of pre

    African Journals Online (AJOL)

    mrmrsolayiwola

    2012-05-01

    May 1, 2012 ... pubertal male rabbits fed with graded level of blood- ... The effects of feeding graded levels of blood wild sunflower forage meal ... and serum biochemical parameters in rabbit were studied. ... (Cheeke et al., 1986), high in protein, low in cholesterol ..... assay of nutritional anaemia (dietary deficiency of iron,.

  20. Diagnostic utility of clinical and biochemical parameters in ...

    African Journals Online (AJOL)

    Diagnostic utility of clinical and biochemical parameters in pancreatic head malignancy patients with normal carbohydrate antigen 19-9 levels. Xiaoli Jin1, Yulian Wu2. 1. Department of Surgery, Sir Run Run Shaw Hospital College of Medicine, Zhejiang University, 3 Qingchun. Road East, Hangzhou, Zhejiang Province ...

  1. Clinical and Biochemical Parameters of Children and Adolescents Applying Pesticides

    Directory of Open Access Journals (Sweden)

    A Ismail

    2010-06-01

    Full Text Available Background: The primary agricultural product in Egypt is the cotton crop. Children and adolescents work seasonally in the cotton fields applying pesticides. Objectives: To examine the effect of pesticide exposure on clinical and biochemical parameters in children and adolescents applying pesticides. Methods: Male children currently applying pesticides and aged between 9 and 19 years (n = 50 were recruited for this study. They were asked to complete work, health, and exposure questionnaires; examined for any medical and neurological problems with particular attention to sensory and motor functions including cranial nerves, sensory and motor system, and reflexes. From each participant, a blood sample was taken to measure acetylcholinesterase activity, and liver and kidney functions. Children who have never worked in agriculture (n = 50, matched on age, education, and socioeconomic status were also studied and served as controls. Results: More neuromuscular disorders were identified in pesticide applicators than controls. A significant lower level of acetylcholinesterase was found in the applicator group compared to the controls. There was also a significant difference in hematological, renal and hepatic indices in the exposed children compared to the control children. Working more days in the current season and also working more years as a pesticide applicator were both associated with an increase in the prevalence of neuromuscular abnormalities and significant changes in the laboratory tests. Conclusion: Children and adolescent pesticide applicators working in farms of Egypt are at risk of developing serious health problems similar to those of adults.

  2. Studies on some biochemical parameters in viral hepatitis patients

    International Nuclear Information System (INIS)

    El-Sherbiny, E.M.

    2002-01-01

    The present investigation deals with studying liver amino transferases (ALT. AST). Cholesterol and triglycerides. As well as testosterone and protection hormones in blood of Egyptian men infected with hepatitis C virus.hepatitis B virus and mixed B and C viruses. These biochemical parameters were evaluated to be used in diagnosis and prognosis of viral hepatitis. Which considered the most important health problem in Egypt and developing countries. Biochemical analysis were performed using spectrophotometric and radioimmunoassay techniques. All data will be subjected to statistical analysis in order to detect the most suitable biochemical analysis that can be used as specific tests for early diagnosis of viral hepatitis and to detect the parameters that show abnormalities among the different groups of infected patients. The data revealed that AST and ALT levels were increased in all patient groups. Concerning the level of triglycerides, it was increased only in the group of mixed viral hepatitis B and C, while cholesterol showed non-significant changes in all viral hepatitis groups. The sex hormone testosterone was decreased in all infected patients while the prolactin level was increased only in case of patients infected with mixed B and C viruses. However, these abnormal values in such sex hormones play a serious role in male sterility

  3. Dynamics of biochemical parameters of blood serum in kidney injuries

    Directory of Open Access Journals (Sweden)

    Y. L. Podgainiy

    2014-12-01

    Full Text Available Aim. Annually injuries of varying severity are registered in more than 4,5 million people (up to 10% of the population in Ukraine; renal injury in polytrauma is detected in 26,4% of cases and takes 2 – 3 place of injury of the abdominal cavity and retroperitoneal space. In order to study the kidney function and other vital organs systems 108 patients were examined. Methods and results. Laboratory methods (clinical and biochemical parameters of blood and urine tests, ultrasound and CT scans of the kidneys and abdominal organs were used. Conclusion. It was established that polytrauma often occurs in males (73,5% of middle-age. 42% of patients presented renal function violation - nitrogen excretion and 84% of patients had activated blood coagulation in the first 7 – 10 days of injury.

  4. Correlation of radiographic and MRI parameters to morphological and biochemical assessment of intervertebral disc degeneration

    OpenAIRE

    Benneker, Lorin M.; Heini, Paul F.; Anderson, Suzanne E.; Alini, Mauro; Ito, Keita

    2004-01-01

    Degenerative disc disease (DDD) is a common finding in MRI scans and X-rays. However, their correlation to morphological and biochemical changes is not well established. In this study, radiological and MRI parameters of DDD were assessed and compared with morphological and biochemical findings of disc degeneration. Thirty-nine human lumbar discs (L1–S1), age 19–86 years, were harvested from eight cadavers. Within 48 h postmortem, MRIs in various spin-echo sequences and biplanar radiographs of...

  5. Effect of nitrate poisoning on some biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    M. B. Mahmood

    2011-01-01

    Full Text Available The present study was conducted to investigate the toxicity of potassium nitrate on glucose, cholesterol, alanine aminotransferase (ALT, aspartate aminotransferase (AST, and the possible ameliorative effect of ascorbic acid (Vitamin C. Male Wister rats are used as experimental model divided into three groups (each of 6-8 rats and treated for six weeks as follows: Group 1: served as control; Group 2: received 2 % potassium nitrate added to the forage and Group 3: received 2 % potassium nitrate together with 1 % ascorbic acid added to rat's forage. Nitrate treatment in group 2 leads to high significant increase levels of glucose in 3rd, 4th, and 5th weeks, cholesterol level increased significantly in both 4th and 5th weeks, while ALT levels increased in the 4th, 5th and 6th weeks, and AST increased significantly in the 5th and 6th weeks. Addition of ascorbic acid with potassium nitrate, lead to reverse all the parameters nearly to normal. It was concluded that potassium nitrate causes significant toxic effect on some biochemical parameters which was ameliorated by ascorbic acid.

  6. Comparison of seasonal effects on some hematological and biochemical parameters between ewes with subclinical mastitis and healthy ewes

    OpenAIRE

    R.N. Sani; M. Moezifar

    2016-01-01

    This study was conducted to compare the effects of season on some hematological and biochemical parameters in ewes with subclinical mastitis and healthy ewes. A total of 1192 milk and blood samples were collected from ewes between February and April (791 samples in spring), August and October (401 samples in summer). California Mastitis Test (CMT), milk culture, hematologic and serum biochemical parameters were obtained from the medical records. Samples which were positive by bacterial cultur...

  7. The dynamics of blood biochemical parameters in cosmonauts during long-term space flights

    Science.gov (United States)

    Markin, Andrei; Strogonova, Lubov; Balashov, Oleg; Polyakov, Valery; Tigner, Timoty

    Most of the previously obtained data on cosmonauts' metabolic state concerned certain stages of the postflight period. In this connection, all conclusions, as to metabolism peculiarities during the space flight, were to a large extent probabilistic. The purpose of this work was study of metabolism characteristics in cosmonauts directly during long-term space flights. In the capillary blood samples taken from a finger, by "Reflotron IV" biochemical analyzer, "Boehringer Mannheim" GmbH, Germany, adapted to weightlessness environments, the activity of GOT, GPT, CK, gamma-GT, total and pancreatic amylase, as well as concentration of hemoglobin, glucose, total bilirubin, uric acid, urea, creatinine, total, HDL- and LDL cholesterol, triglycerides had been determined. HDL/LDL-cholesterol ratio also was computed. The crewmembers of 6 main missions to the "Mir" orbital station, a total of 17 cosmonauts, were examined. Biochemical tests were carryed out 30-60 days before lounch, and in the flights different stages between the 25-th and the 423-rd days of flights. In cosmonauts during space flight had been found tendency to increase, in compare with basal level, GOT, GPT, total amylase activity, glucose and total cholesterol concentration, and tendency to decrease of CK activity, hemoglobin, HDL-cholesterol concentration, and HDL/LDL — cholesterol ratio. Some definite trends in variations of other determined biochemical parameters had not been found. The same trends of mentioned biochemical parameters alterations observed in majority of tested cosmonauts, allows to suppose existence of connection between noted metabolic alterations with influence of space flight conditions upon cosmonaut's body. Variations of other studied blood biochemical parameters depends on, probably, pure individual causes.

  8. The effects of Islamic fasting on blood hematological-biochemical parameters

    Directory of Open Access Journals (Sweden)

    Mohamad Reza Sedaghat

    2017-06-01

    Conclusion:This study on healthy subjects suggests that fasting could affect some hematological-biochemical parameters but not all of them. Also, these changes in hematological-biochemical parameters were within the normal range and Ramadan fasting seems to be safe for healthy subjects.

  9. Egg quality parameters and blood biochemical profile of six strains ...

    African Journals Online (AJOL)

    Six different poultry strains (Indigenous chicken, Broiler, Turkey, Geese, Duck and Guinea fowl) were studied under extensive system of management to investigate the effect of rearing system on their egg quality and the blood biochemical profile, respectively. Birds used for the study were obtained from four different ...

  10. Differential response of biochemical parameters to EMS and MMS ...

    African Journals Online (AJOL)

    (1964) and biochemical estimations from the liver was done by the method of Sinha (1972) for catalase, Van der Vies (1954) for glycogen and Uchiyama and Mihara (1978) for MDA. Results: The study has revealed that EMS and MMS induced a dose dependent increase in chromosomal aberrations of chromatid type in the ...

  11. Impact of parathion exposure on some biochemical parameters in ...

    African Journals Online (AJOL)

    Nagat Aly

    2014-09-16

    Sep 16, 2014 ... developing field aims to reveal environmental threats before obvious toxic effects such as ... followed by the Student–Newman–Keuls test to determine sig- nificance between ..... open-field activity in chicks. Toxicol Lett 2007 ... ical and serum biochemical changes in mice and protective effect · of vitamin C. J ...

  12. Effects of ramadan fasting on cardiovascular and biochemical parameters

    Directory of Open Access Journals (Sweden)

    M Gupta

    2013-12-01

    Full Text Available Background: Muslims abstain from food and drink from dawn to sunset every day during Ramadan - the holiest month in Islamic lunar calendar.  Methods: The effect of Ramadan fast on body mass index (BMI, blood pressure (BP, fasting blood glucose (FBG and lipid profile were studied on 100 healthy male, adult Muslim volunteers. All parameters were recorded one week before the onset and then in the last week of Ramadan month and compared.  Results: There was no statistically significant effect on BMI, and systolic or diastolic blood pressures (p>0.05. However, fasting blood glucose (FBG, serum total cholesterol (TC, serum triglycerides (TG were significantly decreased (p = 0.000 and high density lipoprotein cholesterol (HDL-C level was significantly (p=0.000 increased in last week of Ramadan fasting compared to pre fasting levels. Conclusion: Our results show beneficial effects of Ramadan fasting on FBG and serum lipid profile. 

  13. Study of Human Biochemical Parameters During and After Ramadan.

    Science.gov (United States)

    Kiyani, Mubin Mustafa; Memon, Aamir Raoof; Amjad, Mian Imran; Ameer, Muhammad Rifaqat; Sadiq, Muhammad; Mahmood, Tariq

    2017-02-01

    Ramadan fasting is obligatory for Muslims each year. They abstain from drinking, eating and intimate relationship between dawn and dusk during this month. Dietary restriction during Ramadan has various biochemical and physiological effects. It is shown to decrease the body weight, glucose and lipid profile. This study aims to analyze the changes in body weight, glucose and lipid profile during Ramadan in physiotherapy students in Pakistan. The study was conducted during June-July 2013 (Hijri year 1434). There were 80 students (50 males and 30 females) recruited in the experiment. They all were healthy adults between the age group of 18-24 (mean age 20.5) years. All subjects underwent a general physical examination and interview, and non-healthy subjects were excluded. On statistical analysis, quantitative data were expressed in terms of mean ± SD and a p value of ≤0.05 was believed statically significant. Paired t test was used to compare the variables. There was a little reduction in body weight (62.7 ± 8.8 vs. 62.3 ± 9.0; p value = 0.009) but a significant decline in glucose level (72.6 ± 12.5 vs. 57.9 ± 10.7; p value = 0.000), low-density lipoprotein level (2.9 ± 0.3 vs. 2.5 ± 0.3; p value = 0.000), total cholesterol (4.6 ± 0.4 vs. 4.2 ± 0.5; p value = 0.000) and triglycerides (1.4 ± 0.5 vs. 1.2 ± 0.5; p value = 0.000) was observed. Moreover, there was some reduction in high-density lipoprotein cholesterol level (1.2 ± 0.3 vs. 1.1 ± 0.3; p value = 0.045). This study shows that Ramadan fasting, a religious obligation for purification of body and soul, resulted in reduced body weight and a positive effect on glucose level and lipid profile.

  14. A comparative biochemical profile of some cyprinids fish in Dukan Lake, Kurdistan-Iraq

    Science.gov (United States)

    Azeez, Darya Mohammed; Mohammed, Sarbaz Ibrahim

    2017-09-01

    The present study was carried out to demonstrate the baseline values for some serum biochemical parameters for 64 adult freshwater fish including seven species belong to family Cyprinidae, have been collected in Dukan Lake, Kurdistan region-Iraq. Fishes were weighed, measured, and collect blood for blood chemistry. Serum biochemical analyses were determined using (Cobas C 311) full automatic chemical analyzer. The result of comparative study of serum biochemical parameters of all Cyprinidae species showed that serum glucose was (459.10±106.99 mg/dl) and direct bilirubin was (0.056±0.021mg/dl) in Barbus grypus, serum total protein (3.511± 0.0484gm/dl) and HDL (133.11±0.4231mg/dl) in Cyprinus carpio, serum cholesterol (338.33±43.923 mg/dl) and LDL (86.11±11.871mg/dl) in Carassius carassius, serum triglyceride (420.0±28.8mg/dl) and ALK (113.93±20.65U/L) in Chondrostoma regium, serum AST and serum ALT in Capoeta trutta, were significantly higher when compared to other species. In a conclusion there is variation in biochemical values among species of same family.

  15. Changes in growth, photosynthetic activities, biochemical parameters and amino acid profile of Thompson Seedless grapes (Vitis vinifera L.).

    Science.gov (United States)

    Somkuwar, R G; Bahetwar, Anita; Khan, I; Satisha, J; Ramteke, S D; Itroutwar, Prerna; Bhongale, Aarti; Oulkar, Dashrath

    2014-11-01

    The study on photosynthetic activity and biochemical parameters in Thompson Seedless grapes grafted on Dog Ridge rootstock and its impact on growth, yield and amino acid profile at various stages of berry development was conducted during the year 2012-2013. Leaf and berry samples from ten year old vines of Thompson Seedless were collected at different growth and berry developmental stages. The analysis showed difference in photosynthetic activity, biochemical parameters and amino acid status with the changes in berry development stage. Higher photosynthetic rate of 17.39 umol cm(-2) s(-1) was recorded during 3-4mm berry size and the lowest (10.08 umol cm(-2) s(-1)) was recorded during the veraison stage. The photosynthetic activity showed gradual decrease with the onset of harvest while the different biochemical parameters showed increase and decrease from one stage to another in both berry and leaves. Changes in photosynthetic activity and biochemical parameters thereby affected the growth, yield and amino acid content of the berry. Positive correlation of leaf area and photosynthetic rate was recorded during the period of study. Reducing sugar (352.25 mg g(-1)) and total carbohydrate (132.52 mg g(-1)) was more in berries as compared to leaf. Amino acid profile showed variations in different stages of berry development. Marked variations in photosynthetic as well as biochemical and amino acid content at various berry development stages was recorded and thereby its cumulative effect on the development of fruit quality.

  16. Oxamyl-induced alterations in hematological and biochemical parameters in rats

    International Nuclear Information System (INIS)

    Fayez, V.

    2003-01-01

    Effect of daily oral doses of 0.9 and 2.5 mg/kg of the carbamate insecticide oxamyl for 16 days on selected hematological and biochemical parameters in male rats was investigated. The weight of animals was significantly decreased compared to controls. The hematological studies revealed significant reduction in red blood cell count, hemoglobin and hematocrit values. Impairment of thyroid function was noticed by elevation of triiodothyronine (T 3 ) and depression of thyroxine (T 4 ). Brain acetylcholinesterase (AchE) was moderately inhibited in the first few days of exposure. However, the results of the parameters investigated indicate a moderate degree of toxicity of oxamyl following oral exposure of the doses selected

  17. The Effect of Ramadan Fasting on Biochemical Parameters in Healthy Thai Subjects.

    Science.gov (United States)

    Ongsara, Sara; Boonpol, Sakulrat; Prompalad, Nussaree; Jeenduang, Nutjaree

    2017-09-01

    Although, the effect of Ramadan fasting on the risks for Cardiovascular Disease (CVD) has been reported in several studies, the results were inconsistent. In addition, the effect of Ramadan fasting on biochemical parameters in Thai subjects has not been evaluated. The aim of this study was to investigate the effect of Ramadan fasting on anthropometry, blood pressure, Fasting Blood Glucose (FBG), lipid profiles, and body composition in healthy Thai subjects. A total of 65 healthy subjects (21 men and 44 women) aged between 19-24 years were randomly recruited. Anthropometry, blood pressure, FBG, Total Cholesterol (TC), Triglyceride (TG), High Density Lipoprotein-Cholesterol (HDL-C), Low Density Lipoprotein-Cholesterol (LDL-C), and body composition were measured before Ramadan, end of Ramadan and after one month of Ramadan. There were no changes in anthropometry, blood pressure, lipid profiles and body composition in both genders before Ramadan, end of Ramadan and after one month of Ramadan. Nevertheless, FBG levels were significantly increased after one month of Ramadan compared with baseline (5.09±0.50 versus 4.83±0.38 mmol/L, p=0.016, respectively) in women. The Ramadan fasting did not affect the lipid, anthropometric and body composition in healthy Thai subjects. However, the increased FBG levels after one month of Ramadan were observed in women. To improve the favourable biochemical parameters after Ramadan fasting, the lifestyle modifications such as, increased intake of healthy diets and increased physical activity should be recommended.

  18. Influence of Fasciola Hepatica on Serum Biochemical Parameters and Vascular and Biliary System of Sheep Liver

    Directory of Open Access Journals (Sweden)

    A Hodžić

    2013-03-01

    Full Text Available Background: The aim of this study was to evaluate the functional capacity of the liver based on the activity of specific enzymes and bilirubin in serum and also to investigate the influence of mechanical and toxic effects of Fasciola hepatica on the structures of the blood vessels and biliary tract in the sheep liver.Methods: Blood samples and liver of 63 indigenous sheep of Pramenka breed, slaughtered in the period from March to December 2009 were used. Based on parasitological findings in the liver, all animals were divided into two groups: control (n=34 and infected group (n=29. For investigation and description of pathological changes in sheep liver, naturally infected with F. hepatica, corrosion cast technique was used.Results: Biochemical analysis of tested parameters showed a significant elevation (P≤0.05 of serum gamma-glutamyl transferase (GGT, total bilirubin (TBIL and direct bilirubin (DBIL in infected sheep group comparing with the control group. No significant differences were observed for activity of aspartate aminotranferase (AST between groups. Vascular and biliary systems of the liver were found to be affected.Conclusion: Results of biochemical analysis are consistent with pathological findings and measuring of tested parameters could be used in early diagnosis of sheep fasciolosis and to test the effectiveness of anthelmintic therapy. Corrosion cast technique is very useful for investigation of pathological changes and neoangiogenesis of vascular and biliary system in sheep liver, caused by mechanical and toxic effects of F. hepatica.

  19. Biochemical Nutritional Parameters in Breast-milk and Plasma of ...

    African Journals Online (AJOL)

    Undernourishment in HIV infected individuals exacerbates immunosuppression, acceleration of HIV replication and CD4 + T cell depletion. The production of human milk (lactogenesis) is dependent on factors in the blood therefore deranged blood parameters in HIV patients are expected to reflect in the components of ...

  20. Infrared spectra in monitoring biochemical parameters of human blood

    International Nuclear Information System (INIS)

    Prabhakar, S; Singh, R A; Jain, N

    2012-01-01

    Infrared spectroscopy is gaining recognition as a promising method. The infrared spectra of selected regions (2000-400cm -1 ) of blood tissue samples are reported. Present study related to the role of spectral peak fitting in the study of human blood and quantitative interpretations of infrared spectra based on chemometrics. The spectral variations are interpreted in terms of the biochemical and pathological processes involved. The mean RNA/DNA ratio of fitted intensities and analytical area as calculated from the transmittance peaks at 1121cm -1 /1020cm -1 is found to be 0.911A.U and 2.00A.U. respectively. The ratio of 1659cm -1 /1544cm -1 (amide-I/amide-II) bands is found to shed light on the change in the DNA content. The ratio of amide-I/amide-II is almost unity (≅1.054) for blood spectra. The deviation from unity is an indication of DNA absorption from the RBC cells. The total phosphate content has found to be 25.09A.U. The level for glycogen/phosphate ratio (areas under peaks 1030cm -1 /1082cm -1 ) is found to be 0.286A.U. The ratio of unsaturated and saturated carbonyl compounds (C=O) in blood samples is in form of esters and the analytical areas under the spectral peaks at 1740cm -1 and 1731cm -1 for unsaturated esters and saturated esters respectively found to be 0.618A.U.

  1. Changes in biochemical, hemodynamic, and dialysis adherence parameters in hemodialysis patients during Ramadan.

    Science.gov (United States)

    Alshamsi, Shaikha; Binsaleh, Fatima; Hejaili, Fayez; Karkar, Ayman; Moussa, Dujana; Raza, Hamad; Parbat, Parkash; Al Suwida, Abdulkareem; Alobaili, Saad; AlSehli, R; Al Sayyari, Abdulla

    2016-04-01

    This paper aimed to study the effect of Ramadan fasting on biochemical and clinical parameters and compliance for dialysis. A prospective multicenter observational cross-sectional study comparing fasting with a non-fasting stable adult hemodialysis patients for demographic and biochemical parameters, compliance with dialysis, inter-dialytic weight gain, pre- and post-blood pressure, and frequency of intradialytic hypotensive episodes was carried out. Six hundred thirty-five patients, of whom 64.1% fasted, were studied. The fasters were younger (53.3 ± 16.2 vs. 58.4 ± 16.1 years; P = 0.001) but had similar duration on dialysis (P = 0.35). More fasters worked (22.0% vs. 14.6%; P = 0.001) and missed dialysis sessions during Ramadan. No differences were noted between groups in sex, diabetic status, or dialysis shift or day. There were no differences in the pre- and post-dialysis blood pressure; serum potassium, albumin or weight gain; diabetic status; sex; and dialysis shift time or days. However, serum phosphorous was significantly higher in the fasting group (2.78 ± 1.8 vs. 2.45 ± 1.6 mmol/L; P = 0.045). There were no intragroup differences in any of the parameters studied when comparing the findings during Ramadan with those in the month before Ramadan. Fasters were significantly younger and more likely to be working, to miss dialysis sessions, and to have higher serum phosphorous levels. No other differences were observed. © 2015 International Society for Hemodialysis.

  2. Analysis of gingival pocket microflora and biochemical blood parameters in dogs suffering from periodontal disease.

    Science.gov (United States)

    Polkowska, Izabela; Sobczyńska-Rak, Aleksandra; Gołyńska, Magdalena

    2014-01-01

    Periodontal diseases in dogs are caused by bacteria colonising the oral cavity. The presence of plaque comprising accumulations of aerobic and anaerobic bacteria leads to the development of periodontitis. Due to the fact that in a large percentage of cases periodontal diseases remain undiagnosed, and consequently untreated, they tend to acquire a chronic character, lead to bacteraemia and negatively impact the health of internal organs. The aim of the present study was to perform a qualitative microbiological analysis of gingival pockets and determine the correlations between selected morphological and biochemical blood parameters and the extent periodontal diseases. Twenty-one dogs treated for periodontal diseases were qualified for the study and subsequently divided into two groups: with 3rd and 4th stage of periodontal disease. Swabs from the patients' gingival pockets were taken for bacteriological testing. Blood was tested for parameters including erythrocyte count, haemoglobin concentration, haematocrit values and leukocyte count. Blood serum was analyzed with respect to the concentrations of alanine transaminase (ALT), aspartate transaminase (AspAT/AST) and urea. The microbiological analysis of gingival pockets indicated the presence of numerous pathogens with a growth tendency in bacterial cultures observed in dogs with advanced-stage periodontal disease. The concentration of biochemical blood markers was significantly higher in dogs with 4th stage of periodontal disease, to compared to the 3rd-stage group. Morphological parameters were not significantly different with the exception of haemoglobin concentration, which was lower in dogs with 4th stage disease. In both groups, elevated leukocyte counts were observed. By conducting a detailed microbiological examination, it is possible to provide a better prognosis, plan adequate treatment and monitor dogs treated for peridontopathy. Copyright © 2014 International Institute of Anticancer Research (Dr. John G

  3. A New Theory for Calculation of Some Biochemical Parameters Concentration in Human Serum

    International Nuclear Information System (INIS)

    Moustafa, K.A.; Amien, A.I.

    2009-01-01

    The serum volumes of the blood samples are varied from one patient to another according to the packed cell volume (PCV %), so if the patient sample has low PCV %, it will have high serum volume and vice versa. To calculate a certain clinical parameter such as glucose in the serum of patients using the conventional calculation, it will give the concentration in units per deci liter serum, while by calculating the concentration according to the ratio of serum volume to the total volume of the blood, it will give different results. Thus, the present study aimed to find a new theory used for calculation of some biochemical parameters concentration taking into consideration the ratio of plasma volume to the total blood volume. The present study was conducted on 122 subjects. These subjects were categorized into 4 groups. Group 1 (G1) comprised 40 healthy subjects as control group, group 2 (G2) comprised 30 low PCV % patients, group 3 (G3) comprised 30 subjects with relatively high PCV % and group 4 comprised 22 diabetic patients. Each group of the previous groups was further subdivided into group a (G a ) and group b (G b ). In the later group, the results were multiplied by the correction factor (V p /V b ), which is the ratio of plasma volume (V p ) to the blood volume (V b ) TSH hormone, glucose, cholesterol, triglycerides, urea, creatinine, uric acid, alanine aminotransferase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) were estimated and the results were multiplied by the correction factor (V p /V b ) to get the results after correction. Compared the results before and after correction, there was a very highly significant (ρ p /V b ), thus we recommended that biochemical parameters results must be calculated as indicated in the present study to obtain actual results which might be useful in the correct diagnosis, monitoring and follow up of the different diseases

  4. Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis

    Directory of Open Access Journals (Sweden)

    Bijan Esmaeilnejad

    2012-03-01

    Full Text Available Babesia ovis plays an important role in causing anemia and kidney dysfunction in affected animals. There are few extensive studies about hematological and biochemical findings of small ruminants' babesiosis caused by B. ovis. The aim of this study was to evaluate the effect of babesiosis on some hematological and biochemical parameters in infected small ruminants with B. ovis. A total of 280 sheep and 122 goats from 40 herds were randomly examined for the presence of B. ovis in blood samples. Of 402 samples, 67 animals (16.7% were positive for B. ovis of which 52 (18.5% were sheep and 15 (12.2% goats, respectively. The infected animals were divided into four subgroups according to parasitemia rates (<1%, 1%, 2%, and 3%. As a control group, 67 uninfected animals were also selected from the same farms. With increase in parasitemia rates, hemoglobin concentration (Hb, packed cell volume (PCV, red blood cells (RBCs, mean corpuscular volume (MCV and mean corpuscular hemoglobin concentration (MCHC significantly decreased (P < 0.05, while, total leukocyte count, number of lymphocyte, monocyte, neutrophil and eosinophil showed a significant increase (P < 0.05. Infected animals presented a significant elevation (P < 0.05 of total proteins and significantly lower level (P < 0.05 of albumin compared to non-infected animals. Significant elevation (P < 0.05 of BUN, creatinine, cholestrol, triglyceride, HDL and LDL level were found with parasitemia progression.

  5. Effects of blood lead level on biochemical and hematological parameters in children with neurological diseases of Western Maharashtra, India.

    Science.gov (United States)

    Pratinidhi, Shilpa A; Patil, Arun J; Behera, Manaskumar; Patil, Maya; Ghadage, Dnyaneshwari P; Pratinidhi, Asha K

    2014-05-01

    Lead is found in small but appreciable quantities in air, soil, drinking water, and food. Exposure to such amounts of lead does not lead to acute lead toxicity but produces subtle effects particularly in children. The aim of this study was to investigate the effects of blood lead level on biochemical and hematological parameters in children with neurological diseases in Western Maharashtra, India, and to estimate the blood lead level by liver and kidney function tests and hematological parameters in children with neurological disorders admitted to the pediatric ward and compare them with healthy controls. In this study, 30 children with various neurological disorders admitted to the pediatric ward of Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India, were compared with 30 age- and sex-matched healthy controls. Four milliliters of venous blood was collected for estimation of blood lead level, and biochemical and hematological parameters were determined using standard methods. Blood lead level was significantly increased in the study group (plead levels, there was a significant difference between the groups. All other biochemical and hematological parameters were not significantly altered in the study group as compared to the control group. Neurologically challenged children are more vulnerable to lead intoxication. It is imperative for the parents to take extra care of their children's food habits and limit hand-to-mouth activities to prevent lead intoxication.

  6. Correlation between serum adiponectin and clinical characteristics, biochemical parameters in Indian women with polycystic ovary syndrome

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    Sunita J Ramanand

    2014-01-01

    Full Text Available Background: Polycystic ovary syndrome (PCOS is a common disorder. PCOS women are at a high risk for insulin resistance and metabolic syndrome (MS. Adiponectin is positively related to insulin sensitivity. It has a preventive role in atherogenesis and MS. The present work was conducted to study the correlation between serum adiponectin levels and clinical characteristics and biochemical parameters in PCOS patients. Materials and Methods: A prospective study in 49 newly diagnosed (as per Rotterdam criteria Indian PCOS women was conducted. PCOS women were clinically examined and investigated for biochemical parameters. Results : The mean serum adiponectin was 12 ± 9.4 μg/mL (range 0.47-45. Hypoadiponectinemia (serum adiponectin <4 μg/mL was present in 22% patients. Age and adiponectin correlated significantly and inversely (r = −0.42, P = 0.027. Overweight/obese patients had lower mean adiponectin levels than normal weight (11.62 ± 9.5 vs 13.58 ± 9.5, P = 0.56. It was significantly lower in patients with acanthosis nigricans (AN as compared with those without AN (8.4 ± 5.9 vs 15 ± 11, P = 0.038. Hirsute patients showed lower mean adiponectin levels than nonhirsute (10 ± 7.3 vs 13 ± 10, P = 0.57. A positive, insignificant correlation was observed between serum adiponectin and cholesterol, low-density lipoprotein, follicle stimulating hormone (FSH, thyroid stimulating hormone, levels. A negative insignificant correlation existed between serum adiponectin and luteinizing hormone (LH, LH: FSH ratio, prolactin, dehydroepiandrosterone, testosterone, triglyceride, high-density lipoprotein, fasting blood glucose, fasting insulin, and Homeostasis Model Assessment. Conclusion: Hypoadiponectinemia is present in one-fifth of women with PCOS. Adiponectin levels decrease as age advances. Low levels of adiponectin possibly contributes to the development of dermal manifestation (AN of insulin resistance.

  7. Biochemical Parameters and Histopathological Findings in the Forced Molt Laying Hens

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    N Mert

    Full Text Available ABSTRACT The aim of this study was to evaluate the effects of forced molting using biochemical parameters and histopathological findings in laying hens. 36 Hyline W36 strain laying hens, 90 weeks old were chosen for this research. Eight of these chickens were randomly selected and placed in a cage as the control group before the molting program began. All the others 28 chickens were used for the forced molting program. Eight laying hens were slaughtered at the end of the molting program named as molting group. The remaining 20 hens were fed for 37 days, weighted and slaughtered when they reached the maximum egg production (80% as postmolting group. Then, blood was analyzed for malondialdehyde, glutathione, catalase, glucose, calcium, phosphorus, albumin, globulin, total protein, triiodothyronine, thyroxine and Vitamin C. The malondialdehyde and glutathione levels of the thyroid and liver tissues were also analyzed along with an examination of the histopathological changes of the liver, ovarium and thyroid glands; and live body, liver, ovarium, thyroid weights and thyroid lengths. In conclusion, it was found that forced molting produces stress and notable side effects in hens, like the oxidant and antioxidant status of the organs, tissue weights and sizes, hormon profiles, blood biochemical and histopathological parameter changes. The activities of thyroid malondialdehyde (p<0.05, liver glutathione (p<0.01, plasma catalase (p<0.001 were significantly decreased in molting group compared to control values, while liver malondialdehyde levels were significantly increased (p<0.001 and thyroid glutathione levels had nonsignificant effect. These levels in molting hens were the first study for veterinary science.

  8. Biochemical Parameters of Guinea Pig Perilymph Sampled According to Scala and Following Sound Presentation

    Science.gov (United States)

    Gershbein, Leon L.; Manshio, Dennis T.; Shurrager, Phil S.

    1974-01-01

    Guinea pigs were exposed to sound varying from 2 to 8 kHz in frequency and 80-100 dB (SPL) in intensity for periods of 1 hr. The biochemical parameters, glucose, sodium, total protein, and the glycolytic enzymes, aldolase, phosphohexose isomerase, and total LDH as well as isozymes of the latter were ascertained for blood serum, perilymph, and, in some instances, cerebrospinal fluid. The three enzymes occurred at lower levels in perilymph as compared to blood serum. Except for a small difference in serum total protein, sound presentation incurred no significant effect on any of the above parameters. Definite differences in several metabolites were discerned for perilymph sampled according to scala and which were independent of the respective acoustical treatments. Thus, as compared to the scale tympani, the scala vestibuli perilymph displayed a higher glucose content and a diminished total LDH level and of the latter isozymes, LDH1 ranged lower and LDH2, higher. As further evidence pointing to cerebrospinal fluid as the possible origin of perilymph, similarities in glucose contents and LDH isozyme patterns were noted for both fluids. PMID:4470918

  9. EVALUATION OF THE IMPORTANCE OF BIOCHEMICAL PARAMETERS IN RELATION TO ULTRASONOGRAPHIC FINDING IN ECTOPIC PREGNANCY DIAGNOSIS

    Directory of Open Access Journals (Sweden)

    Dragan Lončar

    2011-09-01

    Full Text Available The implantation of the fertilized egg outside the uterine cavity leads to the development of ectopic pregnancy. The incidence of ectopic pregnancy is 1/100 births. The most common place of ectopic implantation of the fertilized ovum is the oviduct (98% with predilection for the ampullar part of the Fallopian tube. The aim of this study was to determine the predictive importance of beta-hCG and progesterone concentration compared to ultrasonographic finding in the ectopic pregnancy diagnosis.We examined 24 patients with ectopic pregnancies which we divided according to the days of amenorrhea into two groups: the first group with the total of 28 patients from 16–42 days and another group of 8 patients with amenorrhea longer than 42 days. The control group was comprised of 20 patients with vital intrauterine pregnancy, gestational age of 42-52 days. Blood samples for quantitative determination of hormones were collected on three occasions after 48 hours in the forenoon time in the examined and control group of pregnant women. Ultrasonographic examinations of all pregnant women were carried out immediately after blood sampling, with the trans-vaginal approach using "make loop" option, and measurements with an accuracy of 0.1 mm.Mean values for beta-hCG range from 698-1774 mlU/ml in the first group of pregnant women, and in the second group of 1896 mlU/ml to 4410 mlU/ml with a statistically significant difference compared to the values in the control group (p <0.001. The concentration of progesterone in the first group of women ranging from 41-70 nmol/l, and in the second group of 76-94 nmol/l which is also the statistically significant difference compared to the control group (p<0.002. We have shown that ultrasonographic finding with its parameters reliably predicts the values of biochemical parameters both in normal intrauterine pregnancy and in the case of ectopic pregnancy.Embryo viability and implantation place condition the values of

  10. Effects of Khaya senegalensis leaves on performance, carcass traits, hemtological and biochemical parameters in rabbits

    Science.gov (United States)

    Abdel-Wareth, A. A. A.; Hammad, Seddik; Ahmed, Hassan

    2014-01-01

    One of the challenges facing farmers today is to ensure adequate integration of natural resources into animal feeds. The aim of the present study is to evaluate the effects of Khaya senegalensis (KS) leaves on the performance of growing male rabbits, carcass traits and biochemical as well as hematological parameters. Thirty New Zealand White male growing rabbits were randomly divided into 3 groups (10 rabbits per group). Group I (control) received standard rabbit diet. Rabbits in group II and group III were fed standard rabbit diet supplemented with 35 % and 65 % KS leaves, respectively. All rabbits were fed daily for 25 days. The performance parameters and carcass criteria, including daily body weight gain, final body weight, and the percentage of dressing, were increased in rabbits fed 35 % KS when compared to the control group. Kidney and liver weight ratios increased significantly in group II but dropped in group III. Furthermore, liver enzymes - alanine aminotransferase and aspartate transaminase and kidney function parameters - urea, and creatinine - increased in both group II (significant P<0.05) and in group III (significant P<0.01) when compared to the control group. Moreover, KS leaves induced a significant increase (P<0.05) in the total white blood cell count, the percentage of granulocytes and the platelet count; whereas, the percentage of lymphocytes, red blood cell count, hemoglobin content, mean corpuscular hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin concentration were not statistically significantly changed. This study demonstrates that the performance parameters and carcass traits are improved by the replacement of rabbit's diet with KS leaves. However, KS leaves may adversely affect liver and kidney function in a dose-dependent manner. Therefore, further studies are required to elucidate the maximum tolerable and toxic, as well as lethal doses, and to isolate the pharmacologically active components from KS leaves. PMID

  11. Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

    Directory of Open Access Journals (Sweden)

    M.A.V. Barros

    2018-05-01

    Full Text Available The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8 or a low-protein (LP, 8% protein, n=14 diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05 in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05 at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates were also not different (P>0.05 between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.

  12. Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat.

    Science.gov (United States)

    Qu, Daofeng; Zhou, Xu; Yang, Feng; Tian, Shiyi; Zhang, Xiaojun; Ma, Lin; Han, Jianzhong

    2017-06-01

    A fast, sensitive and effective method based on the blood biochemical parameters for the detection of PSE meat was developed in this study. A total of 200 pigs were slaughtered in the same slaughterhouse. Meat quality was evaluated by measuring pH, electrical conductivity and color at 45min, 2h and 24h after slaughtering in M. longissimus thoracis et lumborum (LD). Blood biochemical parameters were determined in blood samples collected during carcass bleeding. Principal component analysis (PCA) biplot showed that high levels of exsanguination Creatine Kinase, Lactate Dehydrogenase, Aspertate aminotransferase, blood glucose and lactate were associated with the PSE meat, and the five biochemical parameters were found to be good indicators of PSE meat Discriminant function analysis (DFA) was able to clearly identify PSE meat using the five biochemical parameters as input data, and the class model is an effective diagnostic tool in pigs which can be used to detect the PSE meat and reduce economic loss for the company. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Hemato-biochemical parameters of Pesti-des Petits Ruminants (PPR affected goats in Chittagong, Bangladesh

    Directory of Open Access Journals (Sweden)

    Md. Saiful Bari

    2018-06-01

    Materials and methods: A presumptive diagnosis of PPR was done based on clinical signs and symptoms. A structured record keeping sheet was used for the estimation of prevalence and risk factors of PPR in goat. A total of 103 blood samples were collected randomly and analyzed for hemato–biochemical parameters using automated hemo-analyzer. Results: Out of 103 cases, Black Bengal (59% and young goats aging minimum-12 months (43.85% were recognized as highly susceptible to PPR disease. Strong association was found among all the three factors such as age, breed and sex (RR>1. All the values of hematological parameters such as TEC, TLC, Hb, PCV, and DLC were decreased in PPR affected goat as compared to healthy goats except lymphocyte counts, which was increased significantly (P=0.00. The amount of total protein (3.15 gm/L and albumin (16.88 gm/L were reduced drastically in PPR affected goats. Conclusion: The lymphocyte content in blood was significantly increased where as the total protein and albumin percent were decreased in the goats affected with PPR. Moreover, this variation in blood profile due to PPR virus infected in goat might be a good indicator in this disease diagnosis. [J Adv Vet Anim Res 2018; 5(2.000: 211-217

  14. Effect of pulsed electromagnetic field on some biochemical and hematological parameters of female rats

    International Nuclear Information System (INIS)

    Marzook, E.A.

    2006-01-01

    The present study was designed to investigate the effect of exposure to pulsed electromagnetic spectrum on some biochemical and hematological parameters in female albino rats. A group of mature female rats was exposed to 10 pulses of electromagnetic spectrum (frequency 8-12 GHz) 3 times/week for 3 weeks. The untreated group was considered as the control group. At the end of the experiment, serum levels of malondialdehyde, thyroid triiodothyronine and thyroxine (T3, T4), α-feto protein, estradiol, calcium, urea, creatinine and other hematological parameters were estimated. The present data revealed that serum levels of estradiol, malondialdehyde, urea, creatinine, triiodothyronine and thyroxine were elevated in the exposed group while serum calcium was significantly decreased. Non-significant difference was found in the value of α-feto protein between the two groups. The hematological studies revealed that exposure of rats to electromagnetic spectrum induced significant reduction in red blood cells (RBCs), hemoglobin concentration (Hb) and in hematocrit percent (Hct%), while reticulocyte count (Ret %) was elevated in the treated group. Non-significant changes were observed in platelets, leukocyte (WBCs) and lymphocytic counts in the exposed group as compared to the control group

  15. Influence Of Feminine Hormones On Some Biochemical Parameters In Early And Late Menopausal Status

    International Nuclear Information System (INIS)

    KAMAL, A.M.

    2009-01-01

    Forty two healthy Egyptian women were participated in this study to evaluate the effect of early and late menopause on some biochemical and hormonal parameters. The women were divided into three equal groups. The first was the pre-menopausal group with regular menstrual cycle, the second was the late menopausal group comprised the menopausal female less than 5 years and the third group consisted of women that became menopause since 5-9 years. The second and third groups had almost the same age and body weight. After clinical examination, fasting blood samples were collected from all volunteers. Regarding the pre-menopausal women, blood was withdrawn between the 3 rd and the 5 th day post-menstruation (follicular phase). Follicle stimulating hormone (FSH), leutinizing hormone (LH), progesterone, prolactin (PRL), testosterone, leptin and thyroxine (T4) in addition to hemoglobin (Hb), blood glucose, calcium (Ca), inorganic phosphorous (P), magnesium (Mg) and uric acid were determined. Both menopausal groups showed significant increase in FSH and LH and significant decrease in PRL and late menopausal group experienced also multiple significant correlations between FSH, LH and other tested parameters. Prolongation of menopause exhibited decrease in leptin being significant as compared to pre-menopausal women than that experienced menopause at younger age. Due to the relation between leptin hormone and osteoporosis, the results of this investigation denoted that early cessation of annulations (menopause) in Egyptian women may lead to disturbance in bone metabolism causing inhibiting osteoblastogenesis and decreasing bone mass.

  16. Assessing seasonality of biochemical CO2 exchange model parameters from micrometeorological flux observations at boreal coniferous forest

    Directory of Open Access Journals (Sweden)

    T. Vesala

    2008-12-01

    Full Text Available The seasonality of the NEE of the northern boreal coniferous forests was investigated by means of inversion modelling using eddy covariance data. Eddy covariance data was used to optimize the biochemical model parameters. Our study sites consisted of three Scots pine (l. Pinus sylvestris forests and one Norway spruce (l. Picea abies forest that were located in Finland and Sweden. We obtained temperature and seasonal dependence for the biochemical model parameters: the maximum rate of carboxylation (Vc(max and the maximum rate of electron transport (Jmax. Both of the parameters were optimized without assumptions about their mutual magnitude. The values obtained for the biochemical model parameters were similar at all the sites during summer time. To describe seasonality, different temperature fits were made for the spring, summer and autumn periods. During summer, average Jmax across the sites was 54.0 μmol m−2 s−1 (variance 31.2 μmol m−2 s−1 and Vc(max was 12.0 μmol m−2 s−1 (variance 6.6 μmol m−2 s−1 at 17°C. The sensitivity of the model to LAI and atmospheric soil water stress was also studied. The impact of seasonality on annual GPP was 17% when only summertime parameterization was used throughout the year compared to seasonally changing parameterizations.

  17. Effect of feeding cottonseed meal on some hematological and serum biochemical parameters in broiler birds

    Directory of Open Access Journals (Sweden)

    G. Thirumalaisamy

    2016-07-01

    Full Text Available Aim: The study was undertaken to find out the effect of feeding cottonseed meal (CSM on performance of hematological and serum biochemical parameters in broiler birds. Materials and Methods: A 6-week biological trial was carried out with 432-day-old Cobb 400 broiler chicks distributed to nine experimental diets with six replicates, each containing eight chicks. The experimental diets were formulated based on total amino acids (BTAA or based on digestible amino acids (BDAA with or without iron supplementation with two levels of CSM (2% and 4% and control diet based on maize – soybean. The whole blood was subjected to hematological studies. The serum samples were analyzed for protein fractions and lipid profiles. Results: The packed cell volume (PCV value, red blood cell (RBC numbers, and hemoglobin (Hb were lower in iron unsupplemented CSM BTAA or BDAA diets than the control (33.86-35.54 vs. 36.41%, 2.78-2.87 vs. 2.98 × 106/μl, and 10.30-10.70 vs. 10.88%. Supplementation of iron in CSM diets improved the PCV, RBC numbers, and Hb, and the values were comparable to the control. White blood cell numbers, mean corpuscular volume, mean corpuscular Hb (MCH, and MCH concentration values were comparable to the control. The erythrocyte osmotic fragility (EOF was poor in birds fed diets containing up to 4% CSM BTAA or CSM BDAA without iron supplementation (32.02-32.57 vs. 28.77%. Supplementation of iron improved the EOF. The serum cholesterol level did not change with or without iron supplementation. Conclusion: This study suggested that feeding of CSM BTAA or BDAA up to 4% level voiding iron supplementation lowers the hematological parameters, whereas supplementation of iron did not alter serum protein fractions and cholesterol profile; however, it had lowered some hematological parameters, which was rectified by iron supplementation.

  18. Biochemical and Haematological Blood Parameters at Different Stages of Lactation in Cows

    Directory of Open Access Journals (Sweden)

    Cristian Ovidiu COROIAN

    2017-05-01

    Full Text Available The health status of cows is evaluated and depending on haematological and biochemical profile of blood. Nutrition is the main technological factor that can produce profound changes in the metabolic profile in animals (Dhiman et al., 1991; Khaled et al., 1999; Ingvartsen, 2006. Blood parameters analyze can lead to identify if there are errors in nutrition of lactating cows (Payne et al., 1970. The aim of this study was the evaluation of metabolic and biochemical changes that occur during colostrum period and in terms of number of lactations in cows. The biological material was represented by a total of 60 heads of dairy cows from a family farm from Sălaj County, Romania. The cows are all from Holstein breed and presented no clinical signs of any specific pathology. Blood samples were collected from the jugular vein of each cow and analyzed. 10 individuals from each of the six lactations have been randomly selected. Haematological and biochemical parameters showed variations depending on factors analyzed here. In lactation 1 Hb was 7.55±3.05 (g/dl, while in lactation 6 the value was 12.5±2.10 (g/dl. RBC ranged as follows: in lactation 1 - 28.50±2.05 and in lactation 6 - 30.02±2.05. Lymphocytes varied within very wide limits under the influence of lactation: in lactation 1 - 2.8±1.56 and in lactation 6 - 7.55±1.80. The number of lactations and lactation rank have influenced blood biochemical and hematological parameters in dairy cows. Biochemical parameters are influenced by post-partum day, showing the lowest values in the early days of colostral period and the highest in the last few days of the same period.

  19. Stability of Intercellular Exchange of Biochemical Substances Affected by Variability of Environmental Parameters

    Science.gov (United States)

    Mihailović, Dragutin T.; Budinčević, Mirko; Balaž, Igor; Mihailović, Anja

    Communication between cells is realized by exchange of biochemical substances. Due to internal organization of living systems and variability of external parameters, the exchange is heavily influenced by perturbations of various parameters at almost all stages of the process. Since communication is one of essential processes for functioning of living systems it is of interest to investigate conditions for its stability. Using previously developed simplified model of bacterial communication in a form of coupled difference logistic equations we investigate stability of exchange of signaling molecules under variability of internal and external parameters.

  20. Assessment of Abdominal Fat Using High-field Magnetic Resonance Imaging and Anthropometric and Biochemical Parameters.

    Science.gov (United States)

    Al-Radaideh, Ali; Tayyem, Reema; Al-Fayomi, Kholoud; Nimer, Nisreen; Malkawi, Amer; Al-Zu Bi, Rana; Agraib, Lana; Athamneh, Imad; Hijjawi, Nawal

    2016-12-01

    To measure the abdominal subcutaneous fat (SF) and visceral fat (VF) volumes using high-field magnetic resonance imaging (MRI) and to investigate their association with selected anthropometric and biochemical parameters among obese and nonobese apparently healthy participants. A cross-sectional study was conducted by recruiting 167 healthy participants. Abdominal scans were acquired at 3T MRI, and the SF and VF were segmented and their volumes were calculated. Selected anthropometric and biochemical measurements were also determined. A significant difference (P abdominal fat volumes, leptin, resistin, adiponectin and waist circumference. Waist circumferences were measured by tape and MRI. Findings revealed that MRI-measured fat volumes were different between males and females and had a significant (P fat volumes were found to correlate moderately with interleukin-6 and weakly with cholesterol, serum triglyceride and low-density lipoprotein. Except for cholesterol, all measured biochemical variables and abdominal fat volumes in the current study were significantly associated with body mass index. All anthropometric and biochemical parameters showed weak-to-strong associations with the MRI-measured fat volumes. Abdominal fat distribution was different between males and females and their correlations with some lipid profiles were found to be sex dependent. These findings revealed that MRI can be used as an alternative tool for obesity assessment. Copyright © 2016 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.

  1. EVALUATION OF SOME BIOCHEMICAL PARAMETERS IN RABBITS BLOOD AFTER TWO WEEKS EXPOSURE OF EPICATECHIN

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    Katarina Zbynovska

    2014-02-01

    Full Text Available The aim of present study was to analyse biochemical parameters in blood of rabbits after epicatechin administration during two weeks. Animals (adult female rabbits, body weight 4 ± 0.5 kg were divided into four groups: control group (C and experimental groups (E1 – E3. Experimental groups received epicatechin in injectable form at 10 µg.kg-1 in E1, 100 µg.kg-1 in E2 and 1000 µg.kg-1 in E3 for 14 days three times a week. After two weeks of exposure the blood was collected, in blood serum selected biochemical parameters [(glucose, urea, bilirubin, cholesterol, triglycerides, total proteins, calcium (Ca2+, magnesium (Mg2+, phosphorus (P] were analysed by automatic analyzer Microlab 300 (Merck®, Germany. Potassium (K+, sodium (Na+, chlorides (Cl- were analysed by EasyLyte Plus (Medica Corporation, USA. Epicatechin had no significant influence on the observed parameters (P > 0.05. The present study has shown that the serum cholesterol level and triglycerides levels in the E2 and E3 groups were higher than in the control group, however without significant differences (P > 0.05. Two weeks exposure of epicatehin caused also insignificant increase of glucose in all experimental groups in comparison with the control group. Epicatechin had no effect on the others analysed biochemical parameters.

  2. EFFECT OF DEOXYNIVALENOL ON SOME HAEMATOLOGICAL, BIOCHEMICAL AND ANTIOXIDANT PARAMETERS OF PORCINE BLOOD IN VITRO

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    Katarína Zbyňovská

    2013-02-01

    Full Text Available The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical (cholesterol, triglycerides, total protein, urea, calcium and phosphorus and anti- and pro-oxidants parameters (superoxide dismutase - SOD, glutathione peroxidase - GPx and ROS – reactive oxygen species in porcine blood in vitro. Significantly decreased content of total protein in the group with dose of 1000 ng.l-1 DON was observed compared with the control group. In other groups (E1 with 10 ng.l-1 DON and E2 with 100 ng.l-1 slightly lower values were measures in comparison with the control group. PLT significantly decreased in the experimental group E3 when compared with E1, E2, and the control group. Concentration of GPx in porcine blood significantly (P < 0.05 decreased in E1 against the control group. Concentration of SOD significantly (P < 0.05 decreased in group E2 in comparison with E1 group. The highest value of ROS was in E2 group. Other parameters were not influenced by The most important and the most common Fusarium mycotoxin is deoxynivalenol (DON. It occurs predominantly in grains such as wheat, barley, and maize and less often in oats, rice, rye, sorghum and triticale. It has adverse effects on humans, animals, and crops that result in illnesses and economic losses. The aim of the present study was to investigate the effect of DON on some haematological (red blood cells - RBC, white blood cells - WBC, platelets - PLT, haemoglobin - HGB, packed cell volume - PCV and lymphocyte - LYM, biochemical

  3. Effects of methanolic extract of Moringa oleifera leaves on semen and biochemical parameters in cryptorchid rats.

    Science.gov (United States)

    Afolabi, Ayobami Oladele; Aderoju, Hameed Adeola; Alagbonsi, Isiaka Abdullateef

    2013-01-01

    While anti-oxidant effects of Moringa oleifera in much oxidative stress related diseases have been well reported, cryptorchidism on the other hand has been shown to cause oxidative stress. However, study is scanty on the likely role of Moringa oleifera in reducing cryptorchidism-induced oxidative stress in rats has not been studied. The present study looked into the effects of methanolic extract of Moringa oleifera leaves (MEMO) on semen and biochemical parameters in cryptorchid rats. Twenty male albino rats (200-250 g) were randomly divided into 4 groups (n=5 each). Groups A and B were sham-operated and treated with corn-oil and 200 mg/kg of MEMO respectively, while groups C and D were rendered cryptorchid and also treated with corn-oil and 200 mg/kg of MEMO respectively. Cryptorchid rats had lower testicular weight, sperm count, germ cell count, testicular superoxide dismutase (SOD) concentration, testicular total protein and higher testicular malondialdehyde (MDA) concentration compared to sham-operated rats. MEMO had no significant effect on testicular weight and MDA concentration, while it significantly increased sperm count, germ cell count, testicular SOD and total protein in the cryptorchid rats. The present study suggests that MEMO ameliorates cryptorchidism associated germ cell loss and oxidative stress.

  4. Comparison of biochemical parameters of rainbow trout (Oncorhynchus mykiss) reared in two different trout farms'

    Science.gov (United States)

    Karatas, Tayfun

    2016-04-01

    The aim of this study was to compare biochemical parameters of cultured rainbow trouts (Oncorhynchus mykiss, Walbaum, 1972) reared in two different trout farms' (Agri and Erzurum). The average weights of fish were 150±10gr for first station (Agri), 230±10gr for second station (Erzurum). Fishes used in research were randomly caught from pools, and fifteen pieces were used for each group. Fishes were fed with commercial trout feed with 45-50% crude protein twice a day. The levels of AST, ALT, LDL, total cholesterol and triglyceride in the second station (Erzurum) were found to be higher (p<0.05) than that of first station (Agri). Whereas, the levels of HDL in the second station (Erzurum) were found to be lower (p<0.05) than that of first station (Agri). Differences in the levels of total cholesterol and AST, ALT, HDL, LDL, triglyceride may be associated with size, sex, sexual maturity and environmental conditions (temperature, pH, hardness and dissolved oxygen).

  5. Polydatin administration improves serum biochemical parameters and oxidative stress markers during chronic alcoholism: a pilot study.

    Science.gov (United States)

    Pace, Maria Caterina; Passavanti, Maria Beatrice; Aurilio, Caterina; Sansone, Pasquale; Aurilio, Rossella; DE Maria, Salvatore; Lama, Stefania; Federico, Alessandro; Ravagnan, Gianpietro; Caraglia, Michele; Stiuso, Paola

    2015-01-01

    Polydatin, a hydroxystilbene derived from the rhizome of Polygonum cuspidatum, elicits hepatoprotective and neuroprotective effects through its anti-oxidant properties. The present study aimed to determine the effects of oral administration of polydatin in alcoholic patients in order to improve liver biochemical parameters, serum oxidative stress and mental state. We enrolled 20 chronic alcoholic patients hospitalized for rehabilitative therapy. The patients were divided into two groups receiving the following treatment regimes for two weeks: administration of an anti-oxidant nutritional supplement containing glutathione and vitamin C (group 1), or glutathione, vitamin C and polydatin (group 2). The results of the present study show that elevated plasma aspartate aminotransferase and alanine aminotransferase levels in patients after two weeks of alcohol withdrawal were significantly reduced by polydatin (group 2), when compared to group 1. Polydatin also significantly reduced lipid peroxidation levels. Finally, our preliminary data resulting from the analysis of the Mini-Mental Status suggest that polydatin improves cognitive performance. Daily dietary administration of polydatin should be considered for prevention and treatment of liver disease and cognitive impairment in alcoholic patients. Copyright © 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  6. Tartrazine induced changes in physiological and biochemical parameters in Swiss albino mice, Mus musculus

    OpenAIRE

    Arefin, Sayema; Hossain, Mohammad Salim; Akter Neshe, Shamme; Rashid, Md. Mamun Or; Amin, Mohammad Tohidul; Hussain, Md. Saddam

    2017-01-01

    Now-a-days synthetic food dyes are being used most commonly as food colorant in confectionaries, drugs and cosmetics. Present study was designed to evaluate the toxic effect of tartrazine, a widely used azo dye, on Swiss Albino mice. Experimental animals were treated with tested dye at a dose level 200mg/kg & 400mg/kg body weight along with normal diet. Various physiological and biochemical parameters were assessed to study the toxic effect of tartrazine. Our study rev...

  7. Haematological and Biochemical Parameters during the Laying Period in Common Pheasant Hens Housed in Enhanced Cages

    Directory of Open Access Journals (Sweden)

    Petra Hrabčáková

    2014-01-01

    Full Text Available The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P<0.05 in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P<0.05. At the end of the laying period, an increase (P<0.05 was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P<0.05 were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.

  8. Influence of Dietary Irradiated Curcuma ionga (Turmeric) on Physiological and Biochemical Parameters of Growing Rats

    International Nuclear Information System (INIS)

    El-Niely, H.F.G.; El-Shennaway, H.M.; Hamaza, R.G.

    2010-01-01

    Turmeric, (Curcuma ionga), is a dietary antioxidant and has been known since ancient times to possess therapeutic properties. The present investigation examined the correlation between raw and irradiated turmeric powder (at dose levels of 10, 15 and 20 kGy) intake and the physiological effects on organs weight, haematological parameters, indices of liver function and lipid profile in growing Albino male rats. Also, this study was performed to examine the efficacy of radiation processed turmeric powder to modulate the induced hyperlipidaemia in growing Albino male rats. Thirty six male rats were equally and randomly categorized into six groups. Control group fed a reference diet (casein diet), high fat diet was daily received to rats for 6 weeks. Other animals fed daily on high fat diets containing either raw or irradiated turmeric powder (2 g per 100 g diet) at doses 10, 15 or 20 kGy for 6 weeks. The results showed that the relative spleen, kidneys, heart, lungs, and testes weight, and the levels of Hb, PCV and MCHC were not changed in animals fed the experimental diets for 6 weeks. Animals kept on HFD suffered from liver enlargement. Dietary interventions through administrating 2% (w/w) raw or irradiated turmeric powder at 20 kGy normalized the liver size as compared with those received reference diet. Meanwhile, the results revealed that rats fed on high fat diet significantly increased plasma AST, ALT, TC and TG and significant decrease was observed in HDL. Meanwhile, feeding rats on diet containing of either raw or irradiated turmeric powder at 10, 15 and 20 kGy induced a significant improvement in the above mentioned parameters. These results imply that irradiated turmeric powder at 20 kGy can offer protection against the biochemical changes as a consequence of hyperlipidaemic food and contribute to the regulation of lipid metabolism safely

  9. Evaluation of the effect of conventionally prepared swarna makshika bhasma on different bio-chemical parameters in experimental animals

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    Sudhaldev Mohapatra

    2011-01-01

    Full Text Available Swarna makshika (chalcopyrite bhasma (SMB has been used for different therapeutic purposes since long in Ayurveda. The present study is conducted to evaluate the effect of conventionally prepared SMB on different bio-chemical parameters in experimental animals, for providing scientific data base for its logical use in clinical practice. The genuine SMB was prepared by following classical techniques of shodhana and marana most commonly used by different Ayurvedic drug manufacturers. Shodhana was done by roasting raw swarna makshika with lemon juice for three days and marana was performed by 11 putas . The experimental animals (rats were divided into two groups. SMB mixed with diluted honey was administered orally in therapeutic dose to Group SMB and diluted honey only was administered to vehicle control Group, for 30 days. The blood samples were collected twice, after 15 days and after 30 days of drug administration and different biochemical investigations were done. Biochemical parameters were chosen based on references from Ayurvedic classics and contemporary medicine. It was observed that Hb% was found significantly increased and LDL and VLDL were found significantly decreased in Group SMB when compared with vehicle control group. This experimental data will help the clinician for the logical use of SMB in different disease conditions with findings like low Hb% and high LDL, VLDL levels.

  10. Hematological parameters in relation to age, sex and biochemical values for mute swans (Cygnus olor).

    Science.gov (United States)

    Dolka, B; Włodarczyk, R; Zbikowski, A; Dolka, I; Szeleszczuk, P; Kluciński, W

    2014-06-01

    The knowledge of the correct morphological and biochemical parameters in mute swans is an important indicator of their health status, body condition, adaptation to habitat and useful diagnostic tools in veterinary practice and ecological research. The aim of the study was to obtain hematological parameters in relation to age, sex and serum biochemistry values in wild-living mute swans. We found the significant differences in the erythrocyte count, hematocrit, hemoglobin concentration and erythrocyte sedimentation rate in relation to age of mute swans. There were no differences in hematological values between males and females. The leukogram and H/L ratio did not vary by age and sex in swans. Among of biochemical parameters the slightly increased AST, ALP, CK, K, urea, decreased CHOL and TG values were recorded. As far as we know, this is the first study in which the morphometric parameters of blood cells in mute swans were presented. We found extremely low concentration of lead in blood (at subthreshold level). No blood parasites were found in blood smears. The analysis of body mass and biometric parameters revealed a significant differences dependent on age and sex. No differences in the scaled mass index were found. Our results represent a normal hematologic and blood chemistry values and age-sex related changes, as reference values for the mute swan.

  11. Amelioration of some biochemical parameters in irradiated male albino rats by garlic

    International Nuclear Information System (INIS)

    El-masry, F.S.H.; El-sayed, N.M.; Hussein, A.H.

    2005-01-01

    Garlic extract has various medical effects on the treatment of many diseases as hypertension, atherosclerosis, inflammation and diabetes. The alteration of the biochemical parameters in blood serum of irradiated rats may play an important role in determining the pathogenesis of radiation exposure. Many of the damaging effects of ionizing radiation are mediated by reactive free radicals. This study was designed to evaluate the protective role of garlic against gamma irradiation (5Gy) induced biochemical disorders in rats. Samples were collected at 1, 7 and 14 days post-irradiation. Lipid peroxide content (malondialdehyde), cholesterol, HDL-C, LDL-C, fatty acids, glucose, insulin, glycogen, haemoglobin, ferritin andiron were estimated.Garlic was orally administered to rats (100 mg/kg body weight) for 14 days before exposure to single dose of gamma irradiation at dose level 5 Gy. The data revealed significant increase in the levels of lipid peroxide, cholesterol, LDL-cholesterol, fatty acids, insulin, glucose and iron accompanied with significant decrease in the levels of HDL-cholesterol, glycogen, haemoglobin and ferritin due to radiation exposure. Administration of garlic alone to the rats caused nonsignificant changes in the estimated parameters indicating its safe use, but the treatment with garlic to rats before radiation exposure ameliorated the changes induced by gamma irradiation and tended to normalize their levels.It could be concluded that garlic administration may has a beneficial role in restoring the biochemical disorders induced by 5 Gy gamma irradiation

  12. Effect of toxoplasmosis and brucellosis on some biochemical parameters in ewes

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    N. A. J. Al- Hussary

    2010-01-01

    Full Text Available The present study was conducted to evaluate the effects of infection of ewes with toxoplasmosis and/or brucellosis on someserum biochemical parameters. Ninety six samples of blood were collected from aborted ewes at different stages of gestation,suspected to be infected with toxoplasmosis and /or brucellosis from different regions in Nineveh governorate. The percentageof toxoplasma and brucella infection depending on Latex Agglutination Test (LAT and Rose Bengal Test were 21.88% and23.96% respectively. The results of biochemical analysis showed that infection with toxoplasma caused significant elevation ofserum activities of aspartate aminotransferase (AST, alanine aminotransferase (ALT and lactate dehydrogenase (LDH. Aswell as the concentrations of glucose, total protein and copper. Where as the activity of alkaline phosphatase (ALP and theconcentration of calcium and zinc were reduced. Infection with brucellosis caused a significant increased in serum AST, ALTand LDH, ALP and creatin phosphokinase (CPK activities, and glucose, zinc and cholesterol concentrations. While serumcalcium and total protein concentration were decreased. Association of toxoplasma and brucella infection induced significantelevation of serum AST, ALT and LDH and CPK activities and the concentrations of glucose and copper with significant reduction in serum ALP activity and both calcium and zinc concentrations. It was concluded from this study that infection ofewes with toxoplasma and/or brucella caused changes in some biochemical parameters in the serum.

  13. Cardamom (Elettaria cardamomum perinatal exposure effects on the development, behavior and biochemical parameters in mice offspring

    Directory of Open Access Journals (Sweden)

    Gasem Mohammad Abu-Taweel

    2018-01-01

    Full Text Available Cardamom is a strong antioxidant plant, so it is called the queen of spices. In the present study, we explored the potentials of cardamom on developmental, learning ability and biochemical parameters of mice offspring. Thirty pregnant mice were allocated to three groups of ten animals in each. Groups Π and Ш received pilsbury's Diet containing 10 and 20% of cardamom (w/w respectively, whereas Group I used as control. Cardomom was administered from the first day of pregnancy and was continued until post-natal day 15 (PD 15 and thereafter the mothers were switched to plain pilsbury's Diet. During the weaning period, three pups in each litter were color marked from the others, and were subjected to various tests (Physical assessment such body weight and eye opening and hair appearance; the neuromaturation of reflexes like righting, rotating, and cliff avoidance reflexes; learning ability and memory retention; estimation of monoamines neurotransmitters like dopamine and serotonin, non-enzymatic oxidative stress such as TBARS and GSH in forebrain at different ages of pups. The results indicated that the body weight gain was declining significantly. Hair appearance and eyes opening were delayed significantly. Righting, rotating, and cliff avoidance reflexes were delayed in treated animals. Exposure to cardamom led to enhance learning and memory retention as compared to control. Monoamines (DA, 5-HT and GSH were elevated, whereas TBARS was inhibited significantly. In conclusion, perinatal cardamom exposure enhanced learning and memory as compared to control. Cardamom and its benefit compounds were transported via placenta or/and milk during lactation. Cardamom needs more researches to investigate its benefits on other kinds of behavior.

  14. Cardamom (Elettaria cardamomum) perinatal exposure effects on the development, behavior and biochemical parameters in mice offspring.

    Science.gov (United States)

    Abu-Taweel, Gasem Mohammad

    2018-01-01

    Cardamom is a strong antioxidant plant, so it is called the queen of spices. In the present study, we explored the potentials of cardamom on developmental, learning ability and biochemical parameters of mice offspring. Thirty pregnant mice were allocated to three groups of ten animals in each. Groups Π and Ш received pilsbury's Diet containing 10 and 20% of cardamom (w/w) respectively, whereas Group I used as control. Cardomom was administered from the first day of pregnancy and was continued until post-natal day 15 (PD 15) and thereafter the mothers were switched to plain pilsbury's Diet. During the weaning period, three pups in each litter were color marked from the others, and were subjected to various tests (Physical assessment such body weight and eye opening and hair appearance; the neuromaturation of reflexes like righting, rotating, and cliff avoidance reflexes; learning ability and memory retention; estimation of monoamines neurotransmitters like dopamine and serotonin, non-enzymatic oxidative stress such as TBARS and GSH in forebrain at different ages of pups). The results indicated that the body weight gain was declining significantly. Hair appearance and eyes opening were delayed significantly. Righting, rotating, and cliff avoidance reflexes were delayed in treated animals. Exposure to cardamom led to enhance learning and memory retention as compared to control. Monoamines (DA, 5-HT) and GSH were elevated, whereas TBARS was inhibited significantly. In conclusion, perinatal cardamom exposure enhanced learning and memory as compared to control. Cardamom and its benefit compounds were transported via placenta or/and milk during lactation. Cardamom needs more researches to investigate its benefits on other kinds of behavior.

  15. BIOCHEMICAL AND MORPHOMETRIC PARAMETERS OF PRE-LARVAE OF THREE SALMONIDS SPECIES AT ONE-DAY AGE

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    Ye. Barylo

    2016-06-01

    Full Text Available Purpose. To study and analyze the morphometric and some biochemical parameters of pre-larval brown trout, rainbow trout and brook trout in post-embryonic period under the conditions of "Rybnyi Potik” farm in the Transcarpathian region for further use of the obtained data in scientific and practical works related to the cultivation of the juveniles of valuable salmonid species. Methodology. One-day free embryos (pre-larvae of brown trout, rainbow trout and brook trout we used as study materials. Morphometric parameters we studied by the methods of N. O. Lange, E. N. Dmitrieva. The content of total lipids was determined in accordance with Folch. in the tissuesm, which were taken for biochemical studies. Separate classes of lipids were received by thin layer chromatography. Findings. We carried out a comparative analysis of morphometric measurements and biochemical parameters of one-day pre-larval brown trout, rainbow trout and brook trout based on the obtained data. We investigated morphometric and biochemical specific features of pre-larvae in post-embryonic period and showed the species differences of morphometric measurements. Significant differences were observed between the content of lipids in the body and yolk sac of free embryos. In particular, a higher content of phospholipids and triglycerides was observed in the body of brook trout compared to brown trout. We also recorded higher contents of mono- and diacylglycerols, free cholesterol, non-etherified fatty acids (NEFA, triacylglycerols and cholesterol esters in the yolk sac of brook trout. Compared to brown trout, rainbow trout had a significant increase in mono- and diacylglycerols, free cholesterol and NEFA in both body and yolk sac as well higher levels of total lipids, triacylglycerols and cholesterol esters were registered in yolk sac. Originality. For the first time we carried out and compared the specific features of pre-larval brown trout, rainbow trout and brook trout in the

  16. Nickel accumulation and its effect on growth, physiological and biochemical parameters in millets and oats.

    Science.gov (United States)

    Gupta, Vibha; Jatav, Pradeep Kumar; Verma, Raini; Kothari, Shanker Lal; Kachhwaha, Sumita

    2017-10-01

    With the boom in industrialization, there is an increase in the level of heavy metals in the soil which drastically affect the growth and development of plants. Nickel is an essential micronutrient for plant growth and development, but elevated level of Ni causes stunted growth, chlorosis, nutrient imbalance, and alterations in the defense mechanism of plants in terms of accumulation of osmolytes or change in enzyme activities like guiacol peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Ni-induced toxic response was studied in seedlings of finger millet, pearl millet, and oats in terms of seedling growth, lipid peroxidation, total chlorophyll, proline content, and enzymatic activities. On the basis of germination and growth parameters of the seedling, finger millet was found to be the most tolerant. Nickel accumulation was markedly lower in the shoots as compared to the roots, which was the highest in finger millet and the lowest in shoots of oats. Plants treated with a high concentration of Ni showed significant reduction in chlorophyll and increase in proline content. Considerable difference in level of malondialdehyde (MDA) content and activity of antioxidative enzymes indicates generation of redox imbalance in plants due to Ni-induced stress. Elevated activities of POD and SOD were observed with high concentrations of Ni while CAT activity was found to be reduced. It was observed that finger millet has higher capability to maintain homeostasis by keeping the balance between accumulation and ROS scavenging system than pearl millet and oats. The data provide insight into the physiological and biochemical changes in plants adapted to survive in Ni-rich environment. This study will help in selecting the more suitable crop species to be grown on Ni-rich soils.

  17. The Hypertrophic Marchigiana: physical and biochemical parameters for meat quality evaluation

    Directory of Open Access Journals (Sweden)

    F. M. Sarti

    2010-04-01

    Full Text Available The aim of this study was to evaluate the meat quality of double muscled Marchigiana young bulls characterized by different genotypes for the hypertrophy: normal and mutated (heterozygous. Calpain and calpastatin activities were determined to verify the state of aging meat on a sample of Longissimus thoracis muscle (XIII thoracic rib taken at slaughtering (0h and after 24 hours (24h. After 14 days of aging, another sample of muscle was taken to evaluate physical and chemical parameters of meat quality. The results showed a better meat quality of mutated animals respect normal animals. Another interesting result was the correlation between the biochemical parameters and some physical parameters, such as WBS (Warner Bratzler Shear Force, CL (Cooking loss. These results showed the relationship between the proteolytic activity of calpain system and meat tenderness.

  18. Population based reference intervals for common blood haematological and biochemical parameters in the akuapem north district.

    Science.gov (United States)

    Koram, Ka; Addae, Mm; Ocran, Jc; Adu-Amankwah, S; Rogers, Wo; Nkrumah, Fk

    2007-12-01

    To estimate the reference intervals for commonly used blood haematology and biochemical parameters in an adult (18-55yrs) population of residents of Mampong Akuapem. This was a population based cross sectional study of a randomly selected sample of the adult population of Mampong. The sample was selected from an updated census list of the Mampong area. Median values (95% range) for measured parameters were established as follows: Haemoglobin, (males) 14.2 g/dl (females) 12.0 g/dl Alanine aminotransferase (ALT), (female) 19.6 U/L (males) 26.1 U/L and Creatinine, (males) 108 mmol/L (females) 93 mmol/L. In comparison to reference values that are commonly used in Ghana, the haemoglobulin levels from this study were lower, and liver function parameters higher. This could be a result of genetic or environmental differences and calls for the need to establish site specific reference values applicable to our population.

  19. Effect of dietary inclusion of sugar syrup on production performance, egg quality and blood biochemical parameters in laying hens

    Directory of Open Access Journals (Sweden)

    Ahmed S. Hussein

    2018-03-01

    Full Text Available The effects of dietary inclusion of sugar syrup on quality of egg, cholesterol level, production performance, serum total protein and blood biochemical parameters were evaluated in laying hens. A total of 300 commercial Lohmann LSL hens (30 weeks of age were randomly distributed into 3 dietary treatments which consisted of a normal corn diet containing corn–soy and 2 diets containing 5% and 10% sugar syrup. Each treatment was replicated 5 times (n = 20. Egg production, feed intake, body weight and egg weight of laying hens fed different diets were recorded. The experiment lasted for 20 weeks. The Haugh unit scores of hens fed diets with sugar syrup were significantly increased (P < 0.05 compared with the control treatment. The sugar syrup had no significant effect on liver enzymes, total protein, blood glucose and creatinine in all treatments. The eggs laid by hens fed sugar syrup diets had lower cholesterol level (P < 0.05 compared with those laid by hens fed the control diet. Electrophoresis analysis showed that comparable electrophoretic patterns were noticed between serum proteins of treatment groups. From the results, it can be concluded that sugar syrup diets and corn diets have similar effects on feed intake, body weight, production of eggs and blood biochemical parameters in layer hens, which suggests sugar syrup can be used as an energy source for replacing part of corn in poultry layer diets. Keywords: Cholesterol, Sugar syrup diet, Egg, Egg quality, Protein

  20. [Simulation of vegetation indices optimizing under retrieval of vegetation biochemical parameters based on PROSPECT + SAIL model].

    Science.gov (United States)

    Wu, Ling; Liu, Xiang-Nan; Zhou, Bo-Tian; Liu, Chuan-Hao; Li, Lu-Feng

    2012-12-01

    This study analyzed the sensitivities of three vegetation biochemical parameters [chlorophyll content (Cab), leaf water content (Cw), and leaf area index (LAI)] to the changes of canopy reflectance, with the effects of each parameter on the wavelength regions of canopy reflectance considered, and selected three vegetation indices as the optimization comparison targets of cost function. Then, the Cab, Cw, and LAI were estimated, based on the particle swarm optimization algorithm and PROSPECT + SAIL model. The results showed that retrieval efficiency with vegetation indices as the optimization comparison targets of cost function was better than that with all spectral reflectance. The correlation coefficients (R2) between the measured and estimated values of Cab, Cw, and LAI were 90.8%, 95.7%, and 99.7%, and the root mean square errors of Cab, Cw, and LAI were 4.73 microg x cm(-2), 0.001 g x cm(-2), and 0.08, respectively. It was suggested that to adopt vegetation indices as the optimization comparison targets of cost function could effectively improve the efficiency and precision of the retrieval of biochemical parameters based on PROSPECT + SAIL model.

  1. Effect of taking dietary supplement on hematological and biochemical parameters in male bodybuilders an equation model

    Science.gov (United States)

    Meamar, Rokhsareh; Maracy, Mohammad; Nematollahi, Shahrzad; Yeroshalmi, Shemouil; Zamani-Moghaddam, Ali; Ghazvini, Mohammad Reza Aghaye

    2015-01-01

    Background: The improved physical action following administration of supplements to bodybuilders was supported by changes in laboratory parameters. Despite the fact that these supplements are sometimes associated both advantage and side effects, this study were conducted for the purpose of evaluating the possible effects of some commonly used supplements in bodybuilders on the hematological and biochemical parameters. Materials and Methods: In this study, we included 40 male bodybuilders as cases and 40 controls in the age group of 20-40 years. They used different kinds of supplements for 1 year. In general, all the supplements used were classified into two groups: hormonal and non-hormonal. Laboratory tests were requested for evaluation of hematological and biochemical parameters. Results: In an equation model, we found that weight (P = 0.024), duration of bodybuilding (P bodybuilders. The available supplements are unchecked and not approved by the US Food and Drug Administration (FDA). More studies should be designed for a better and precise administration of each supplement in athletes. PMID:26793253

  2. Effect of ascorbic acid on morphological and biochemical parameters in tomato seedling exposure to salt stress

    Directory of Open Access Journals (Sweden)

    Krupa-Małkiewicz Marcelina

    2015-06-01

    Full Text Available The aim of this study is to determine the effect of both NaCl and KCl alone and in comparison to AsA on the morphological and some biochemical parameters of Oxheart and Vilma cultivars of tomato under laboratory and field conditions. A combination of salt applied in the laboratory experiment caused a significant effect on seed germination and root and shoot length and a significant reduction of Chl a, Chl b and Car contents in 14-day-old tomato seedlings. However, seedlings of cultivar Vilma were characterised by higher tolerance to applied salt stress.

  3. Set-base dynamical parameter estimation and model invalidation for biochemical reaction networks.

    Science.gov (United States)

    Rumschinski, Philipp; Borchers, Steffen; Bosio, Sandro; Weismantel, Robert; Findeisen, Rolf

    2010-05-25

    Mathematical modeling and analysis have become, for the study of biological and cellular processes, an important complement to experimental research. However, the structural and quantitative knowledge available for such processes is frequently limited, and measurements are often subject to inherent and possibly large uncertainties. This results in competing model hypotheses, whose kinetic parameters may not be experimentally determinable. Discriminating among these alternatives and estimating their kinetic parameters is crucial to improve the understanding of the considered process, and to benefit from the analytical tools at hand. In this work we present a set-based framework that allows to discriminate between competing model hypotheses and to provide guaranteed outer estimates on the model parameters that are consistent with the (possibly sparse and uncertain) experimental measurements. This is obtained by means of exact proofs of model invalidity that exploit the polynomial/rational structure of biochemical reaction networks, and by making use of an efficient strategy to balance solution accuracy and computational effort. The practicability of our approach is illustrated with two case studies. The first study shows that our approach allows to conclusively rule out wrong model hypotheses. The second study focuses on parameter estimation, and shows that the proposed method allows to evaluate the global influence of measurement sparsity, uncertainty, and prior knowledge on the parameter estimates. This can help in designing further experiments leading to improved parameter estimates.

  4. Effect of aspartame on biochemical and oxidative stress parameters in rat blood

    Directory of Open Access Journals (Sweden)

    Prokić Marko D.

    2015-01-01

    Full Text Available Aspartame (ASP is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT, aspartate aminotransferase (AST and lactate dehydrogenase (LDH, as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO and lipid peroxides (LPO in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041

  5. Assessment of Hematological and Biochemical parameters with extended D-Ribose ingestion

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    Frelich Angela

    2008-09-01

    Full Text Available Abstract D-ribose, a naturally occurring pentose carbohydrate, has been shown to replenish high- energy phosphates following myocardial ischemia and high intensity, repetitive exercise. Human studies have mainly involved short-term assessment, including potential toxicity. Reports describing adverse effects of D-ribose with prolonged ingestion have been lacking. Therefore, this study assessed the toxicity of extended consumption of D-ribose in healthy adults. Nineteen subjects ingested 20 grams/Day (10 grams, twice a Day of ribose with serial measurements of biochemical and hematological parameters at Days 0, 7, and 14. No significant toxic changes over the 14-day assessment period occurred in complete blood count, albumin, alkaline phosphatase, gamma glutamyltransferase, alanine amiotransferase, and aspartate aminotransferase. However, D-ribose did produce an asymptomatic, mild hypoglycemia of short duration. Uric acid levels increased at Day 7, but decreased to baseline values by Day 14. D-ribose consumption for 14 days appears not to produce significant toxic changes in both hematological and biochemical parameters in healthy human volunteers.

  6. A new trend to determine biochemical parameters by quantitative FRET assays.

    Science.gov (United States)

    Liao, Jia-yu; Song, Yang; Liu, Yan

    2015-12-01

    Förster resonance energy transfer (FRET) has been widely used in biological and biomedical research because it can determine molecule or particle interactions within a range of 1-10 nm. The sensitivity and efficiency of FRET strongly depend on the distance between the FRET donor and acceptor. Historically, FRET assays have been used to quantitatively deduce molecular distances. However, another major potential application of the FRET assay has not been fully exploited, that is, the use of FRET signals to quantitatively describe molecular interactive events. In this review, we discuss the use of quantitative FRET assays for the determination of biochemical parameters, such as the protein interaction dissociation constant (K(d)), enzymatic velocity (k(cat)) and K(m). We also describe fluorescent microscopy-based quantitative FRET assays for protein interaction affinity determination in cells as well as fluorimeter-based quantitative FRET assays for protein interaction and enzymatic parameter determination in solution.

  7. EVALUATION OF HEMATOLOGY AND BIOCHEMICAL PARAMETERS OF FREE-LIVING EUROPEAN BROWN HARE

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    Miriam Trusinová

    2012-12-01

    Full Text Available The objective of our study was to determine blood hematological and serum chemistry values for a population of free-living European brown hares and to investigate differences related to age. The European brown hare (Lepus europaeus is a species of hare naturally occurring in northern, central and western parts of Europe and southwestern Asia. The blood samples were collected from 25 hares (15 adults and 10 juveniles shot during regular hunting seasons in 4 hunting grounds located in Danube lowland. Following parameters were analyzed: WBC, LYM, GRA, RBC, HGB, HCT, MCV, MCH, MCHC on blood samples, and glucose, total proteins, urea, bilirubin, cholesterol, Ca, P, Na, K, Cl on serum. WBC count was higher in adults (2.86.109.l-1 than in young hares (2.71.109.l-1. RBC count and HGB concentration were higher in young hares (RBC=8.94.1012.l-1, HGB=184.43 g.l-1 than in adults (RBC=8.38.1012.l-1, HGB=177.59 g.l-1. No significant differences were found in other hematology parameters. Biochemical indicators of age were evaluated and an increased content of glucose in adult individuals was found (a = 9.39 mmol.l-1, j = 8.48 mmol.l-1. The increased level of urea in blood serum was observed as almost significant in juvenile individuals (a = 6.11 mmol.l-1, j = 6.94 mmol.l-1, but also reduction of bilirubin was observed (a = 5.19 mmol.l-1, j = 4.23 mmol.l-1. Other monitored biochemical parameters were fairly balanced. All evaluated hematology and serum chemistry parameters of brown hares were within the physiological range with relatively low differences.

  8. Interactive effects of salinity stress and nicotinamide on physiological and biochemical parameters of Faba bean plant

    International Nuclear Information System (INIS)

    Abdelhamid, Magdi T; Sadak, Mervat Sh; Schmidhalter, Urs; El Saady, Abdel Kareem M.

    2013-01-01

    solutes concentrations in seeds of salinity treated plants. Nicotinamide not only neutralized the effect of salinity stress but resulted in a significant improvement in physiological and biochemical parameters as well as the concentrations of soluble sugars, proline, amino acids, and total N and other mineral contents.

  9. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica).

    Science.gov (United States)

    Binaii, Mohammad; Ghiasi, Maryam; Farabi, Seyed Mohammad Vahid; Pourgholam, Reza; Fazli, Hasan; Safari, Reza; Alavi, Seyed Eshagh; Taghavi, Mohammad Javad; Bankehsaz, Zahra

    2014-01-01

    The present study investigated the effects of different dietary nettle (Urtica dioica) levels on biochemical, hematological and immunological parameters in beluga (Huso huso). Fish were divided into 4 groups before being fed for 8 weeks with 0%, 3%, 6% and 12% of nettle. The blood samples were collected on week 4 and 8. The use of nettle did not significantly change the mean cell volume, mean cell haemoglobin, lymphocytes, eosinophils, albumin, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lysozyme activity on week 4 and 8. After 4 weeks, the total red blood cell (RBC) and hematocrit (Ht) showed a significant increase in 12% nettle group compared to the 3% nettle and control groups but haemoglobin (Hb) had a significant change in 12% nettle compared to the control. At the same time was not found a significant change in the mean cell haemoglobin concentration (MCHC), total white blood cell (WBC), neutrophils, respiratory burst activity (RB), total immunoglobulin (Ig) and total protein (TP), triglyceride (Tri) and cholesterol (Chol). After 8 weeks, the fish treated with nettle exhibited significantly increase in neutrophil and Hb levels compared to the control and between treatment groups, 12% nettle group shown the highest Hb while RBC and Hct values significantly rose in fish fed by 12% compared to the control. Supplementing 6% and 12% nettle increased the WBC and MCHC compared to the other groups. The group fed 12% showed a highly significant difference in RB, TP and Ig after 8 weeks. However, Tri and Chol were significantly decreased in the juvenile beluga fed by the 6% and 12% nettle diet compared to the other groups. The results suggest that by using this herb there will be an improvement in hemato-biochemical parameters and immune function of juvenile beluga.

  10. Genetic parameters for the prediction of abdominal fat traits using blood biochemical indicators in broilers.

    Science.gov (United States)

    Zhang, H L; Xu, Z Q; Yang, L L; Wang, Y X; Li, Y M; Dong, J Q; Zhang, X Y; Jiang, X Y; Jiang, X F; Li, H; Zhang, D X; Zhang, H

    2018-02-01

    1. Excessive deposition of body fat, especially abdominal fat, is detrimental in chickens and the prevention of excessive fat accumulation is an important problem. The aim of this study was to identify blood biochemical indicators that could be used as criteria to select lean Yellow-feathered chicken lines. 2. Levels of blood biochemical indicators in the fed and fasted states and the abdominal fat traits were measured in 332 Guangxi Yellow chickens. In the fed state, the genetic correlations (r g ) of triglycerides and very low density lipoprotein levels were positive for the abdominal fat traits (0.47 ≤ r g  ≤ 0.67), whereas total cholesterol, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) showed higher negative correlations with abdominal fat traits (-0.59 ≤ r g  ≤ -0.33). Heritabilities of these blood biochemical parameters were high, varying from 0.26 to 0.60. 3. In the fasted state, HDL-C:LDL-C level was positively correlated with abdominal fat traits (0.35 ≤ r g  ≤ 0.38), but triglycerides, total cholesterol, HDL-C, LDL-C, total protein, albumin, aspartate transaminase, uric acid and creatinine levels were negatively correlated with abdominal fat traits (-0.79 ≤ r g  ≤ -0.35). The heritabilities of these 10 blood biochemical parameters were high (0.22 ≤ h 2  ≤ 0.59). 4. In the fed state, optimal multiple regression models were constructed to predict abdominal fat traits by using triglycerides and LDL-C. In the fasted state, triglycerides, total cholesterol, HDL-C, LDL-C, total protein, albumin and uric acid could be used to predict abdominal fat content. 5. It was concluded that these models in both nutritional states could be used to predict abdominal fat content in Guangxi Yellow broiler chickens.

  11. Effect of salinity on growth, biochemical parameters and fatty acid composition in safflower (carthamus tinctorius l.)

    International Nuclear Information System (INIS)

    Javed, S.; Bukhari, S.A.; Mahmood, S.; Iftikhar, T.

    2014-01-01

    The aim of the present project is to investigate the effect of salinity on growth, biochemical parameters and fatty acid composition in six varieties of safflower as well as identification of stress tolerant variety under saline (8 d Sm-1) condition. It was observed that salinity significantly decreased the dry weight and fresh weight of safflower varieties. Nitrate reductase (NRA) and nitrite reductase (NiRA) activities were also reduced in response to salinity in all safflower genotypes but Thori-78 and PI-387820 showed less reduction which could be a useful marker for selecting salt tolerant varieties. Under salinity stress, total free amino acids, reducing, non reducing sugars and total sugars increased in all varieties. Accumulation of sugars and total free amino acids might reflect a salt protective mechanism and could be a useful criterion for selecting salt tolerant variety. Comparison among safflower genotypes indicated that Thori-78 and PI-387820 performed better than the others and successful in maintaining higher NRA, NiRA and other metabolites thus were tolerant to salinity. Differential effect upon fatty acid synthesis was observed by different varieties under salinity stress but PI-170274 and PI-387821 varieties better maintained their fatty acid composition. It can be concluded from present studies that biochemical markers can be used to select salinity tolerant safflower varieties. (author)

  12. Effect of Mucuna pruriens on semen profile and biochemical parameters in seminal plasma of infertile men.

    Science.gov (United States)

    Ahmad, Mohammad Kaleem; Mahdi, Abbas Ali; Shukla, Kamla Kant; Islam, Najmul; Jaiswar, Shyam Pyari; Ahmad, Sohail

    2008-09-01

    To investigate the impact of Mucuna pruriens seeds on semen profiles and biochemical levels in seminal plasma of infertile men. Prospective study. Departments of Biochemistry and Obstetrics and Gynecology, King George's Medical University, Lucknow, India. Sixty normal healthy fertile men (controls) and 60 men undergoing infertility screening. High-performance liquid chromatography assay procedure for quantitation of vitamin A and E in seminal plasma. Biochemical parameters in seminal plasma, namely lipids, fructose, and vitamin C, were estimated by standard spectrophotometric procedures. Before and after the treatment, seminal plasma lipid profile, lipid peroxide, fructose, and antioxidant vitamin levels were measured. Treatment with M. pruriens significantly inhibited lipid peroxidation, elevated spermatogenesis, and improved sperm motility. Treatment also recovered the levels of total lipids, triglycerides, cholesterol, phospholipids, and vitamin A, C, and E and corrected fructose in seminal plasma of infertile men. Treatment with M. pruriens increased sperm concentration and motility in all the infertile study groups. Oligozoospermic patients recovered sperm concentration significantly, but sperm motility was not restored to normal levels in asthenozoospermic men. Furthermore, in the seminal plasma of all the infertile groups, the levels of lipids, antioxidant vitamins, and corrected fructose were recovered after a decrease in lipid peroxides after treatment. The present study is likely to open new vistas on the possible role of M. pruriens seed powder as a restorative and invigorating agent for infertile men.

  13. ASSOCIATION OF SOME BIOCHEMICAL-GENETIC MARKERS WITH THE REPRODUCTION PARAMETERS OF THE BOTOSANI KARAKUL EWES

    Directory of Open Access Journals (Sweden)

    GH. HRINCĂ

    2008-10-01

    Full Text Available The paper describes some associative aspects of various biochemical-genetic markers with the reproduction activity in ewes of the Botosani Karakul breed. The two most important reproduction parameters (fecundity and prolificacy were analyzed according to the genotypes or phenotypes of polymorph systems (haemoglobin, transferrin, albumin and blood potassium of females. The relationship between reproduction data and genetic markers in ewes was quantified both for each genotype (phenotype and for each mating couple type made up depending on the genotype (phenotype of the couple partners (homozygous x homozygous, homozygous x heterozygous or heterozygous x heterozygous. All these associative aspects and their results are discussed for each polymorph genetic system. The ewes which are heterozygous at different genetic marker loci are more fertile and more prolific than the ewes which are homozygous at the levels of these loci. The highest conception and lambing rates resulted from the mating couples in which both partners were heterozygous and the least lambs were obtained from the mating couples in which both partners were homozygous; the fertility and prolificacy had intermediate values in heterogeneous mating couples (heterozygotes x homozygotes but they were nearer to the case in which both mating couple partners were heterozygous. The sheep breeding field can benefit by the contribution of biochemical-genetic markers to optimize the selection criteria with a view to increasing the reproduction capacity of this species.

  14. Radiation-Induced Changes in Some Biochemical Parameters of the Haemopoietic Tissue of Rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Izak, G.; Karsai, A.; Eylon, L. [Haematology Research Laboratory, Hadassah University Hospital (Israel); Hebrew University-Hadassah Medical School, Jerusalem (Israel)

    1968-08-15

    Bone-marrow and reticulocyte suspensions obtained from rabbits following massive haemorrhage were irradiated by a cobalt source with doses ranging between 1000-10 000 rad. The radiation-induced injury to the nucleic acid synthesizing apparatus and to the haem-and protein-producing activity of the bone-marrow cells, as well as the damage to the haemoglobin synthesizing system of the reticulocytes, was investigated shortly after exposure. The amino-acid incorporation activity into protein was substantially reduced, together with all the other parameters measured in the bone-marrow cells, while globin synthesis was not affected at the same radiation dose in the reticulocytes. On the other hand, incorporation of haem precursors into protoporphyrin and of radio iron into protoporphyrin was inhibited both in the nucleated erythroid precursors and in the reticulocytes, more so in the former than in the latter. The implications of these findings and possible means to repair the biochemical injury are discussed. (author)

  15. Effect of induced epilepsy on some biochemical parameters in female rats

    Directory of Open Access Journals (Sweden)

    J.S. H. Ali

    2012-01-01

    Full Text Available The activity of cholinesterase and some biochemical parameters of blood such as glucose, cholesterol and phospholipids were estimated in 52 epilepsy induced females of Wister albino rats. Animals of this experiment were divided into two groups, group (I regarded as control and group (II administrated subcutaneously by pentylenetetrazole 100mg/kg and divided in to three sub-groups according to the time of samples collection 3 hrs, 24 hrs and 1 week. The results revealed that epilepsy induction caused a significant inhibition of serum cholinesterase activity 3 hrs after induction while in the brain, the activity of cholinesterase was significantly increased after 24 hrs Serum glucose level was significantly elevated after 3 hrs and 24 hrs of induction, total cholesterol and phospholipids were not changed. From the results obtained in this study, it can be concluded that epilepsy caused significant changes in cholinesterase activity in brain and serum in addition to the glucose level in the serum.

  16. THE STUDY OF SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH MULTIPLE MYELOMA

    Directory of Open Access Journals (Sweden)

    Bularda Morozan Mihai

    2010-09-01

    Full Text Available This paper presents the results of some biochemical parameters obtained in the laboratory of “Elena Beldiman” Emergency Hospital Barlad, and in the”Dr. Stoian –Dr. Ungureanu”Private Medical Practice on 10 patients diagnosed with multiple myeloma during 2005-2011. Numerous researches over serum proteins with the help of various methods ( refractometry have shown the presence of some changes in protein fractions of blood serum. These changes express the change of the normal ratio between these fractions, that means disorders in colloidal structure of blood. The results of the electrophoresis analysis, also in accordance with those from the scholarly literature, show that the changes in the condition of the blood serum are due to the increase in the globulins fractions which is linked to the growth of the immunological processes, the antibodies synthesis being especially closely linked to gammaglobulinic fraction

  17. Assessment of some biochemical and hematological parameters in treated with the organophosphorus insecticide cyanox

    International Nuclear Information System (INIS)

    ElMahdy, A.A.; Fayz, V.; Hassanin, M.M.

    2002-01-01

    The effect of single acute oral doses of 29 and 58 mg/kg of cyanox (an organophosphorus pesticide) on selected biochemical and hematological parameters in male rats were investigated. This compound inhibited blood acetylcholine esterase (AChE) significantly, over 72 hours post-treatment with the higher dose level. Serum alkaline phosphatase (ALP) was significantly depressed, 48 hr after treatment with 58 mg/kg. Serum bilirubin was elevated significantly after 48 and 24 hr of treatment with 29 and 58 mg/kg, respectively. Serum cholesterol increased significantly over 48 hr post-treatment with both dose levels.Hypertriglyceridemia was noticed only in the higher dose group. Serum total lipids were increased significantly at both dose levels. A slight treatment-related effect was observed in serum creatinine level. The hematological investigations revealed the occurrence of macrocytic normochromic anemia that was more evident in the lower dose group

  18. Effect of pregnancy on some biochemical parameters in Sahel goats in semi-arid zones.

    Science.gov (United States)

    Sandabe, U K; Mustapha, A R; Sambo, E Y

    2004-05-01

    The effects of pregnancy on some biochemical parameters were studied using 20 sexually mature, cycling goats with weight range 20-25 kg. They were randomly separated into two groups of 10 animals each. In one group, oestrus was detected while going round with a buck in the morning and evening; a single buck on detection of oestrus mated the does and the does were tagged as pregnant after confirmation of non-return of oestrus. The other group was kept cycling and tagged as non-pregnant. The mean serum glucose concentration in pregnant does was 63.35 +/- 7.70 mg/dl, significantly lower than 71.59 +/- 1.14 mg/dl for non-pregnant does (p 0.05), as did the liver enzymes (ALT, AST). Therefore this study showed that low serum glucose and high cholesterol levels are features of mid to late pregnancy in Sahel goats.

  19. Nutritional status of low birth weight infants in Makkah region: Evaluation of anthropometric and biochemical parameters.

    Science.gov (United States)

    Kensara, Osama Adnan; Azzeh, Firas Sultan

    2016-04-01

    To assess the nutritional status of low birth weight infants from Makkah area immediately after birth. The prospective study was conducted between October and December 2012 at Al-Noor Speciality Hospital, Makkah, Saudi Arabia, and comprised low birth weight infants who were divided into three equal groups according to their birth weight: group A (low birthweight1501-2500gm), group B (very low birthweight1001-1500gm), and group C (extremely low birth weight 0.05). Normal serum phosphorus, potassium and magnesium levels and mild hypocalcaemia were observed in all infants. However, hypernatraemia was significantly evident (pnutritional status in terms of low anthropometric and abnormal biochemical measures. It was not possible to correlate the birth weight of the neonates to the parameters of the complete blood tests.

  20. THE EFFECTS OF VEGETABLE LECITHIN ON SOME SANGUINE BIOCHEMICAL PARAMETERS AT LACTATING SHEEP AND GOATS

    Directory of Open Access Journals (Sweden)

    D. DRINCEANU

    2008-05-01

    Full Text Available From a complex experiment of establishing the effects of lecithin supplemented in the diet of small ruminants, in this paper we present the influence of lecithin on some sanguine biochemical parameters at lactating sheep and goat. The researches were made on a experimental group formed by 5 sheep from łurcana breed and 5 goats from Crapatina breed, which were fed in the first experimental period with a base ratio (Br formed by 1.5 kg of hill hay and 0.2 kg concentrate forage mixture, in the second experimental period the Br was supplemented with 100 g lecithin/day/animal. At the end of each experimental period were taken blood samples to establish some biochemical parameters from sanguine serum. The supplementation of base ration with 100 g lecithin/day/animal at lactating sheep and goats did not produced significant changes to values of total proteins (TS, albumin, creatinine, urea and total bilirubins (TB from sanguine serum. Contrary to expectation the cholesterol and triglycerides were higher than normal both in sheep (from 82 mg/dl to 83 mg/dl for cholesterol and from 23 mg/dl to 45 mg/dl for triglycerides but specially in goats (from 59 mg/dl to 68 mg/dl for cholesterol and from 24 mg/dl to 55 mg/dl for triglycerides. Were registered significant increment of activity of alkaline phosphatase and in γ – GT and a decrease of plasmatic activity of pancreatic α-amylase both in sheep and goats.

  1. Effect of fermented biogas residue on growth performance, serum biochemical parameters, and meat quality in pigs

    Directory of Open Access Journals (Sweden)

    Xiang Xu

    2017-10-01

    Full Text Available Objective This study investigated the effect of fermented biogas residue (FBR of wheat on the performance, serum biochemical parameters, and meat quality in pigs. Methods We selected 128 pigs (the mean initial body weight was 40.24±3.08 kg and randomly allocated them to 4 groups (1 control group and 3 treatment groups with 4 replicates per group and 8 pigs per pen in a randomized complete block design based on initial body weight and sex. The control group received a corn-soybean meal-based diet, the treatment group fed diets containing 5%, 10%, and 15% FBR, respectively (abbreviated as FBR5, FBR10, and FBR15, respectively. Every group received equivalent-energy and nitrogen diets. The test lasted 60 days and was divided into early and late stages. Blood and carcass samples were obtained on 60 d. Meat quality was collected from two pigs per pen. Results During the late stage, the average daily feed intake and average daily gain of the treatment groups was greater than that of the control group (p<0.05. During the entire experiment, the average daily gain of the treatment groups was higher than that of the control group (p<0.05. Fermented biomass residue did not significantly affect serum biochemical parameters or meat quality, but did affect amino acid profiles in pork. The contents of Asp, Arg, Tyr, Phe, Leu, Thr, Ser, Lys, Pro, Ala, essential amino acids, non-essential amino acids, and total amino acids in pork of FBR5 and FBR10 were greater than those of the control group (p<0.05. Conclusion These combined results suggest that feeding FBR could increase the average daily gain and average daily feed intake in pigs and the content of several flavor-promoting amino acids.

  2. Biochemical and seminal parameters of lambs fed palm kernel cake under grazing system

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    Lopes César Mugabe

    Full Text Available ABSTRACT This study aimed to assess the effects of palm kernel cake on semen quality and biochemical parameters of Santa Inês lambs. A total of 40 animals with 24.10±2.72 kg body weight and five months old were assigned in a completely randomized design into four groups and 10 replicates. The animals were subjected to four levels of palm kernel cake (0, 15, 30, and 45% based on dry matter. The trial lasted 90 days foregone by 15 days for adaptation. Blood samples were collected every 45 days from jugular vein using vacuum tubes without anticoagulant. Total serum cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, and very-low-density lipoprotein were assessed. Once the animals reached puberty at a mean age of 225 days, the semen samples were collected by electroejaculator once a week for three sequence weeks and assessed for volume, color, aspect, wave motion, motility, sperm concentration, sperm vigor, total of spermatozoa per ejaculate, viable spermatozoa per mL, and sperm morphology. The data were subjected to analysis of variance and followed by regression analysis. Non-parametric data were analysed by Kruskal-Wallis test. Total cholesterol, high-density lipoprotein, triglycerides, and very-low-density lipoprotein were linearly increased. There was no difference for low-density lipoprotein. Diets did not affect mass motility, sperm motility, vigor, total spermatozoa per ejaculate, viability sperm per mL, and minor and total sperm defects. Sperm concentration increased linearly. Negative quadratic effects were observed for major sperm defects. Supplementation of diets with palm kernel cake up to 45% on dry matter enhance biochemical parameters and do not impair the qualitative variables of lamb sperm.

  3. Shape, size, and robustness: feasible regions in the parameter space of biochemical networks.

    Directory of Open Access Journals (Sweden)

    Adel Dayarian

    2009-01-01

    Full Text Available The concept of robustness of regulatory networks has received much attention in the last decade. One measure of robustness has been associated with the volume of the feasible region, namely, the region in the parameter space in which the system is functional. In this paper, we show that, in addition to volume, the geometry of this region has important consequences for the robustness and the fragility of a network. We develop an approximation within which we could algebraically specify the feasible region. We analyze the segment polarity gene network to illustrate our approach. The study of random walks in the parameter space and how they exit the feasible region provide us with a rich perspective on the different modes of failure of this network model. In particular, we found that, between two alternative ways of activating Wingless, one is more robust than the other. Our method provides a more complete measure of robustness to parameter variation. As a general modeling strategy, our approach is an interesting alternative to Boolean representation of biochemical networks.

  4. Glutathione Enhancer Protects Some Biochemical and Haematological Parameters from the Effect of Electromagnetic Field

    International Nuclear Information System (INIS)

    Marzook, E.A.; Marzook, F.A.

    2016-01-01

    The Present study was designed to study the effect of exposure to electromagnetic field (EMF), emitted from a cellular tower for mobile phone on some biochemical and haematological parameters in male albino rats and to evaluate of the possible protective role of the antioxidant glutathione enhancer on the studied parameters. Three groups of rats were studied, the control (unexposed), the exposed and the treated exposed groups. Exposed groups were subjected to EMF at frequency of 900 MHz, with a peak power of about 60 W, power density of 0.05 mW/ cm"2 at the site of exposure for 24 h/ day for 8 weeks, at the same time treated group was supplied with oral injection of glutathione enhancer three times weekly. At the end of experiment, serum levels of thyroid stimulating hormone (TSH), calcium (Ca), uric acid, malondialdehyde (MDA) beside, the activity of lactate dehydrogenase (LDH) were measured also, some haematological parameters were estimated. Impairment of TSH and serum calcium was expressed by a decrease in serum levels of the exposed group. Meanwhile, serum level of MDA, uric acid and the activity of LDH were increased by exposure. The haematological studies revealed that, exposure to electromagnetic spectrum induced significant reduction in red blood cell counts (RBC's), and haemoglobin concentration (Hb), meanwhile reticulocyte count (Ret) was elevated. The leucocyte counts (WBC's) and platelets count were not affected by exposure. The study demonstrated that glutathione enhancer can attenuate the side effects of exposure to electromagnetic field.

  5. Predicting hepatic steatosis and liver fat content in obese children based on biochemical parameters and anthropometry.

    Science.gov (United States)

    Zhang, H-X; Xu, X-Q; Fu, J-F; Lai, C; Chen, X-F

    2015-04-01

    Predictors of quantitative evaluation of hepatic steatosis and liver fat content (LFC) using clinical and laboratory variables available in the general practice in the obese children are poorly identified. To build predictive models of hepatic steatosis and LFC in obese children based on biochemical parameters and anthropometry. Hepatic steatosis and LFC were determined using proton magnetic resonance spectroscopy in 171 obese children aged 5.5-18.0 years. Routine clinical and laboratory parameters were also measured in all subjects. Group analysis, univariable correlation analysis, and multivariate logistic and linear regression analysis were used to develop a liver fat score to identify hepatic steatosis and a liver fat equation to predict LFC in each subject. The predictive model of hepatic steatosis in our participants based on waist circumference and alanine aminotransferase had an area under the receiver operating characteristic curve of 0.959 (95% confidence interval: 0.927-0.990). The optimal cut-off value of 0.525 for determining hepatic steatosis had sensitivity of 93% and specificity of 90%. A liver fat equation was also developed based on the same parameters of hepatic steatosis liver fat score, which would be used to calculate the LFC in each individual. The liver fat score and liver fat equation, consisting of routinely available variables, may help paediatricians to accurately determine hepatic steatosis and LFC in clinical practice, but external validation is needed before it can be employed for this purpose. © 2014 The Authors. Pediatric Obesity © 2014 World Obesity.

  6. Blood biochemical parameters in male American mink (Neovison vison before and during the breeding season

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    Bogdan Lasota

    2014-05-01

    Full Text Available This study was aimed at selected blood biochemical parameters in the male American mink before (September-November and during the breeding (January-March season. Blood from 143 Black and Sapphire male mink at one year age was collected. The plasma was assayed for the concentration of glucose, total protein, cholesterol, HDL and LDL fractions, triacylglycerides (TG, and the activity of ALT and AST. Concentrations of glucose, protein, total and HDL/LDL cholesterol, and AST activity were generally slightly higher during the breeding season than during the non-breeding season, but remained within the reference range. In the case of ALT activity and TG concentration, the relations were reversed. The parameters studied in the Sapphire mink showed greater variation, both in- and out of the season. In Black and Sapphire males of the American mink, the studied parameters revealed slightly higher values during the breeding season than the non-breeding season. This will vary depending on the color variety. The decrease in TG concentration during the breeding season may indicate an increased energy demand due to ambient temperature falls, and/or may be a sign of increased energy consumption associated with physical exertion during mating. Consideration should be given to the nutrition of male mink during the breeding season.

  7. Dynamics of Clinical and Biochemical Parameters in Patients with Liver Cirrhosis Under the Influence of Complex Therapy with Ursodeoxycholic Acid

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    M.I. Shved

    2013-11-01

    Full Text Available It was studied dynamics of clinical and biochemical parameters in patients with liver cirrhosis under the influence of complex treatment using ursosan. It is found that the inclusion of ursosan in complex treatment improves clinical and laboratory parameters, significantly reduces the manifestations of general inflammatory liver syndrome, which prevents the progression of the disease.

  8. Influence of NaCl on Biochemical Parameters of Two Cultivars of Stevia rebaudiana Regenerated in vitro

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    Sharuti Rathore

    2014-05-01

    Full Text Available Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. It affects plant morpho-physiology and ultimately leads to reduction in productivity. It is essential to test important medicinal plants for their salinity tolerance as research efforts aim to explore economic benefits under saline conditions. Keeping in view the importance of Stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two Stevia genotypes. Two node microcuttings were subjected to MS media supplemented with different NaCl concentrations (0, 25, 50, 75, 100, 125 mM. Chlorophyll amount was observed to be decreased as compared to sugars, proline and phenols with increased salt concentrations.

  9. THE VARIATION OF SOME BIOCHEMICAL PARAMETERS FROM FRESH COW CHEESE CREAM TYPE WITH ADDED AROMATIC PLANTS

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    CRISTIANA DIACONESCU

    2007-10-01

    Full Text Available Recent advanced studies in the field of cheese fabrication technology include, amongother, the usage of aromatic plants and spices as a mean of improving theorganoleptic properties and as a mean of decreasing the lipidic peroxidationphenomenon. For this purpose, three types of fresh cow cheese produced by S.C.Marion Invest Trade 94 SRL, experimentally added with dill, savory and rosemary,were comparatively studied from a chemical point of view (pH, acidity, humidity, ashand a biochemical point of view(raw fat, total proteins, total cholesterol, acidphosphatase’s enzymatic activity, superoxide dismutase’s enzymatic activity, calciumcontent.From a chemical point of view, no significant differences appeared between the creamcheese types analyzed, excepting the pH( lower in the cheeses with dill and rosemaryFrom a biochemical point of view, two phenomenon can be observed: a significantdecrease of the acidic phosphatase activity in the cheeses with added dill androsemary ( positively correlating with the pH value and decreased values of the SODactivity in all cheese types.

  10. Investigation of liver tissue and biochemical parameters of adult wistar rats treated with Arctium lappa L.

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    Fabrícia Souza Predes

    2009-04-01

    Full Text Available This study was carried out to evaluate the effects of Arctium lappa L. (burdock on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST, alanine transaminase (ALT, gamma glutamyl transferase (GGT, total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.A bardana (Arctium lappa L é uma planta trazida do Japão e aclimatada no Brasil, e é extensamente utilizada na medicina popular em todo mundo. Este estudo foi realizado para avaliar os possíveis efeitos da A. lappa no fígado e nos parâmetros bioquímicos plasmáticos em ratos Wistar adultos. Estes receberam a infusão de bardana nas doses de 10 ou 20 g de folhas secas /L de água, por 40 dias. Não houve alteração significativa nos níveis plasmáticos de albumina, aspartato transaminase (AST, alanina transaminase (ALT, gamma glutamil transferase (GGT, proteínas totais, colesterol total, uréia, ácido úrico, triglicérides, cálcio, fósforo, bilirrubina direta e cloro. A análise morfológica não revelou alterações histopatológicas no fígado. Os dados bioquímicos e morfológicos não indicaram a toxicidade da bardana.

  11. Toxicity of Neem Leaf Extracts (Azadirachta indica A. Juss on Some Haematological, Ionoregulatory, Biochemical and Enzymological Parameters of Indian Major Carp, Cirrhinus mrigala

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    M. Saravanan

    2011-10-01

    Full Text Available In the present study, the median lethal concentration (LC 50 of neem leaf extract to Cirrhinus mrigala for 24 h was found to be 1.035 g l-l. During the study period, the haematological parameters including Hb, Hct, RBC, MCV, MCH and MCHC levels were significantly decreased in neem leaf extract exposed fish when compared to the control fish whereas WBC count was increased. Similarly, plasma Na+ and Cl- levels were significantly lower and K+ level were significantly higher when compared to the control. In biochemical study, elevated plasma glucose and induced protein levels were noticed. The enzymes, glutamate oxaloacetate transaminase (GOT and glutamate pyruvate transaminase (GPT activities were increased significantly in gill, liver and muscle of treated fish compared to that of their control groups. The results of the present investigation suggest that neem leaf extracts affects the hematological, ionoregulatory, biochemical and enzymological parameters of fish and alterations of these parameters can be useful in environmental biomonitoring of neem based products in freshwater environment.Keywords: Azadirachta indica, Acute toxicity, Cirrhinus mrigala, Haematology, Ion regulation, Biochemical and Enzymological parameters.

  12. Effects Of Music Therapy On Clinical And Biochemical Parameters Of Metabolic Syndrome

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    Rajnee

    2011-12-01

    Full Text Available Background: Music therapy is a new approach being used for the management of metabolic abnormalities and stress related illness.Objective: To study the effect of Music therapy on various clinical and biochemical parameters of Metabolic Syndrome.Methods: This cross sectional study was carried out on 100 patients of metabolic syndrome selected randomly. These patients were divided into two equal groups after age, sex adjustment. In control group (group I 50 patients underwent the conventional treatment. 50 patients in study group were treated with supervised music protocol along with conventional treatment. The Body Mass Index, ;Waist-Hip ratio, Blood pressure, Fasting blood sugar were monitored weekly while HbA1c and lipid profile were determined at the baseline and after three months of exposure to music therapy. Statistical analysis was performed by employing student t- test.Results: In the study group there was a significant decrease in BMI (27.18±5.02 to 25.44±3.49 kg/m2, p<0.05, waist hip ratio (0.95±0.05 to 0.93±0.05 cm, p<0.05, Fasting blood sugar (196.00±47.80mg/ dl to152.00±16.19mg/dl , p<0.001, HbA1c (8.41±1.31% to 7.08±0.78 % p<0.001, Systolic Blood Pressure (151.00±12.10 to 136±9.04 mmHg p<0.001, Diastolic Blood Pressure (94±4.80 to 86.44±3.16 mmHg, p<0.01, Mean serum cholesterol (257.80±18.92 to 229.12±17.82mg/dl, p<0.001 and triglycerides (180.86±14.04 to 136.50±8.92mg/dl, p<0.001, LDL (167.97±14.40 to 140.20±15.41mg/dl, p<0.001, and VLDL (33.60±2.88 to 28.04±3.08mg/dl, p<0.001 and increase in HDL (33.32±3.38 to 39.71±3.41mg/dl, p<0.001, when compared with those of control group not receiving the music therapy along with the conventional treatment.Conclusion: The promising outcomes of Music therapy showed that it may be considered as a useful adjunct to conventional treatment in management of the metabolic syndrome. This study advocates music therapy to establish it from a general well being concepts to a

  13. Biochemical parameters in Tubifex tubifex as an integral part of complex sediment toxicity assessment

    Energy Technology Data Exchange (ETDEWEB)

    Smutna, M.; Hilscherova, K.; Paskova, V. [Masaryk Univ., Brno (CZ). RECETOX (Research Centre for Environmental Chemistry and Ecotoxicology); Marsalek, B [Czech Academy of Science, Brno (Czech Republic). Centre for Cyanobacteria and their Toxins

    2008-06-15

    Background, aim, and scope Restoration of lakes and reservoirs with extensive cyanobacterial water bloom often requires evaluation of the sediment quality. Next to the chemical analysis of known pollutants, sediment bioassays should be employed to assess toxicity of the present contaminants and to make predictions of associated risk. Brno reservoir in the Czech Republic is a typical example of water bodies with long-term problems concerning cyanobacterial water blooms. Comprehensive assessment of reservoir sediment quality was conducted since successful reservoir restoration might require sediment removal. An important part of this survey focused on an examination of the utility of Tubifex tubifex and its sublethal biochemical markers for the assessment of direct sediment toxicity. Materials and methods This complex study included chemical analysis of contaminants (heavy metals, organic pollutants), ecotoxicity testing of sediment elutriates (tests with Daphnia magna, Pseudomonas putida, Sinapis alba, Scenedesmus subspicatus), and other parameters. We have tested in more detail the applicability of T. tubifex as a test organism for direct evaluation of contact sediment toxicity. Survival tests after 14 days of exposure were complemented by an assessment of parameters serving as biomarkers for sublethal effects [such as total glutathione content (GSH), activities of the enzymes glutathione transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR)]. The data matrix was subjected to multivariate analysis to interpret relationships between different parameters and possible differences among locations. Results The multivariate statistical techniques helped to clearly identify the more contaminated upstream sites and separate them from the less contaminated and reference samples. The data document closer relationships of the detected sediment contamination with results of direct sediment exposure in the T. tubifex test regarding mortality but namely

  14. Analysis of the hematological and biochemical parameters related to lead intoxication.

    Science.gov (United States)

    Yılmaz, Hınç; Keten, Alper; Karacaoğlu, Emre; Tutkun, Engin; Akçan, Ramazan

    2012-11-01

    In parallel with industrial advancements, number of the occupational diseases secondary to chemical exposure is increasing. The chemical agents in the work places affect various organ and tissue systems, leading to chronic diseases. In this study, the cases diagnosed with occupational disease due to exposure to lead were studied and importance of the environmental forensic sciences on this issue was emphasized. A hundred and ninety patients diagnosed with occupational disease related to lead intoxication in Ankara Occupational Diseases Hospital between 01/01/2009 and 31/12/2009 were included in the study. Twenty cases were used as the controls. Sociodemographic characteristics, serum chemical parameters and hematological parameters of the patients were retrospectively assessed. Mean age of the cases included in the study was 35.3±8.69. Hemoglobin (Hb) (p=0.018) and Mean corpuscular volume (MCV) (plead exposure than in the controls. Gamma glutamyl transferase (GGT) was significantly lower in the patients with lead exposure than in the controls (p=0.002), whereas alkaline phosphatase (ALP) was found higher (plead exposure than in the control group (p=0.01), while Thyrotrophin-stimulating hormone (TSH) levels were lower (plead (Pb) was correlated positively with ALP values and negatively with Hb, MCV and TSH. Considering its effects on the biochemical and hematological parameters, a detailed investigation should be carried out in the cases with lead exposure, which occupies an important place among the occupational diseases. Copyright © 2012 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

  15. Assessment of hemato-biochemical parameters on exposure to low level of deltamethrin in mouse model

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    Anita Tewari

    2014-03-01

    Full Text Available Aim: In this study, sub-acute toxicity of deltamethrin on hematological and biochemical blood parameters of male albino Swiss mice was evaluated. Materials and Methods: Generally, the maximum permissible residue level (MRL of deltamethrin for food products lies between 0.01 to 0.5 mg/kg body weight. So the mice were exposed orally with two doses of pesticide i.e. 0.1 and 0.5 mg/kg body weight. The doses were given on a daily basis for a period of 15 days and 30 days respectively. Ground nut oil was used as control treatment. Samples of blood were collected at the end of the treatment. Hepatotoxicity was evaluated by quantitative analysis of the serum enzymes alanine transaminase (ALT, aspartate transaminase (AST, alkaline phosphatase (ALKP, total bilirubin (TBIL and serum urea. Alterations of hematological parameters were analysed by total leukocyte, differential leukocyte count and hemoglobin levels. Results: Significant increase in the levels of hepatic enzymes (ALT, AST, ALKP were observed for both doses, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations for 0.5 mg/kg body weight dose which is indicated by leukocytosis, lymphocytosis and neutropenia in long duration study. Conclusions: The results indicated that even very low dose of deltamethrin can promote hematological and hepatic alterations. Thus it is imperative to do further studies on the detrimental effect of the low levels of pyrethroid commonly present in our food, which further necessitate the reduction of maximum permissible levels of residual synthetic pyrethroid levels in foods and feed.

  16. Improved Differential Evolution Algorithm for Parameter Estimation to Improve the Production of Biochemical Pathway

    Directory of Open Access Journals (Sweden)

    Chuii Khim Chong

    2012-06-01

    Full Text Available This paper introduces an improved Differential Evolution algorithm (IDE which aims at improving its performance in estimating the relevant parameters for metabolic pathway data to simulate glycolysis pathway for yeast. Metabolic pathway data are expected to be of significant help in the development of efficient tools in kinetic modeling and parameter estimation platforms. Many computation algorithms face obstacles due to the noisy data and difficulty of the system in estimating myriad of parameters, and require longer computational time to estimate the relevant parameters. The proposed algorithm (IDE in this paper is a hybrid of a Differential Evolution algorithm (DE and a Kalman Filter (KF. The outcome of IDE is proven to be superior than Genetic Algorithm (GA and DE. The results of IDE from experiments show estimated optimal kinetic parameters values, shorter computation time and increased accuracy for simulated results compared with other estimation algorithms

  17. Changes in Hematological, Biochemical and Non-specific Immune Parameters of Olive Flounder, , Following Starvation

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    Jong-Hyun Kim

    2014-09-01

    Full Text Available Triplicate groups of fed and starved olive flounder, Paralichthys olivaceus (body weight: 119.8±17.46 g, were examined over 42 days for physiological changes using hematological, biochemical, and non-specific immune parameters. No significant differences in concentrations of blood hemoglobin and hematocrit and plasma levels of total cholesterol, aspartate aminotransferase, alanine aminotransferase, glucose, and cortisol were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, plasma total protein concentrations were significantly lower in starved fish than in fed fish from day 7 onwards. Moreover, plasma lysozyme concentrations were significantly higher in starved flounder from day 21 onwards. This result confirms that the response of olive flounder to short-term (less than about 1.5 months starvation consists of a readjustment of metabolism rather than the activation of an alarm-stress response. The present results indicate that starvation does not significantly compromise the health status of fish despite food limitation.

  18. Protective role of wheat germ oil on some biochemical parameters in irradiated rats

    International Nuclear Information System (INIS)

    Atia, A.I.; Darwish, M.M.; Sallam, M.H.

    2006-01-01

    Wheat germ oil is an organic nutritionally rich vegetable oil. It is an excellent source of vitamin E and essential fatty acids (octacosanol, linoleic and linolenic). The study confirmed the beneficial role of the used anti-oxidation agents as recommended radio-protectors due to their ability of scavenging free radicals produced by ionizing radiation. The efficacy of daily oral treatment of rats with wheat germ oil (10 mg/ Kg body wt) for 15 days to control many of the damaging effects of ionizing radiation when male rats were subjected to fractionated 8 Gy (2 Gy day after day) of gamma irradiation were studied. Blood samples were collected from animals at 10 and 15 days after treatment and/or exposure. In blood, the data obtained revealed that, radiation exposure caused significant increases in levels of malondialdehyde (MDA), triacylglycerol (TG), total cholesterol (TC), low-density lipoproteins (LDL) and glucose at the 10th day. Meanwhile, significant reduction in contents of total protein, albumin, globulins, high-density lipoproteins (HDL), vitamin E and glucose were recorded at the 15th day. Also, the majority of these parameters were estimated in liver tissues. The results revealed that administration of the natural product wheat germ oil partially ameliorated the radiation-induced biochemical disturbances. These effects were explained in the light of the presumed different mechanisms of wheat germ oil actions

  19. Ascorbic acid interference in the measurement of serum biochemical parameters: in vivo and in vitro studies.

    Science.gov (United States)

    Martinello, Flávia; da Silva, Edson Luiz

    2006-04-01

    To investigate the negative interference of ascorbic acid in serum biochemical tests in relation to the dose of vitamin C intake and to the time of blood collection. Healthy volunteers (n = 18) consumed daily doses of vitamin C (0.25-4.0 g) for 1 week and serum parameters were assayed prior to the experiment and on the eighth day of consumption. Blood samples were collected 4, 12 and 24 h after vitamin C intake. Serum levels of ascorbic acid increased significantly after vitamin C ingestion inhibiting urate and total bilirubin tests 4 and 12 h after intake (P cholesterol tests. Addition of ascorbic acid to serum inhibited the urate test to a similar extent to that observed after vitamin C intake. However, after ingesting vitamin C, the interference for the bilirubin test was greater than that of the in vitro interference. Commonly taken doses of supplementary vitamin C interfered negatively with the serum urate test based on the Trinder method, and with bilirubin metabolism.

  20. THE EVOLUTION OF SOME BIOCHEMICAL PARAMETERS IN THE HONEYBEES’ HAEMOLYMPH (A. M. CARPATHICA COLLECTED IN THE INACTIVE SEASON

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    AGRIPINA SAPCALIU

    2008-10-01

    Full Text Available The biochemical analyses of the blood are largely used for the routine diagnosis and especially for the metabolic survey in farm animals. These facts conduct us to the idea that similar analyses, applied on honeybee hemolymph, could be used IN monitoring the healthy state of honeybee colonies. The present studies represent preliminary researches, which aimed to investigate the variability of the main biochemical parameters in the hemolymph of the healthy honeybees (Apis mellifera in inactive season. The researches were carried out on honeybee samples collected from 5 honeybee colonies belonging to a breeding apiary of the Institute for Beekeeping Research and Development from Bucharest. In order to perform the biochemical analyses, the honeybees samples, consisting in 50 individuals on sample (10 individuals/colony were randomly collected and their haemolimph collected, at different time intervals, in inactive season (fall-winter. Totally, there were collected 250 haemolyph samples in a 2 years interval and the following 21 biochemical parameters were analysed: GLU, HDL-c, ALP, T-cho, Tprot, Alb., BUN, LDH, CPK,, Mg, IP, GGT, GOT, GPT, Ca, Cre,, Amy, T–BIL, TG, UA.. The test was carried out after the collection and processing of the samples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique, respectively .During the 2nd part of the inactive season, the values of most biochemical parameters increase in different proportions, their levels being maintained also in the first part of the active seasons (April, May, June.The values obtained for the main studied biochemical parameters in the haemolymph of the healthy honeybees collected from honeybee colonies kept in natural conditions show a highly variable evolution in the two consecutive years of experiments during the inactive season.

  1. Associations of functional and biochemical parameters of endothelial dysfunction in postmenopausal women with a different state of carbohydrate metabolism

    Directory of Open Access Journals (Sweden)

    Lyudmila Aleksandrovna Ruyatkina

    2015-10-01

    Conclusions. We revealed the associations of metabolic, anthropometric, and hemodynamic factors as well as biochemical markers of endothelial dysfunction with microcirculation parameters in various modes of endothelial activity (basal, occlusion, and reperfusion in postmenopausal women according to their carbohydrate metabolism status; we also describe the effect of age on microvasculature vasomotion.

  2. EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS

    Science.gov (United States)

    EFFECTS OF INHALATION OF SOLUBLE METALLIC CONSTITUENTS OF PARTICULATE MATTER ON CARDIOPULMONARY, THERMOREGULATORY, AND BIOCHEMICAL PARAMETERS IN GUINEA PIGS. JP Nolan1, LB Wichers2, J Stanek3, UP Kodavanti1, MCJ Schladweiler1, PA Evansky1, ER Lappi1, DL Costa1, and WP Watkinson1...

  3. Biochemical and anthropometric correlates of bio-electrical impedance parameters in severely malnourished children

    DEFF Research Database (Denmark)

    Girma, Tsinuel; Nielsen, Anne-Louise Hother; Kæstel, Pernille

    2018-01-01

    established using forward selection after comparing models using likelihood ratio test. RESULTS: The sample comprised 55 children with SAM (age 36 ± 24 months; 60% males; 72.7% oedematous) and 80 healthy control children (age 28 ± 15 months; 47.5% males). Oedematous children had the lowest BI parameters...

  4. A Pilot Comparative Study of 26 Biochemical Markers in Seminal Plasma and Serum in Infertile Men

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    Rui-Xiang Feng

    2015-01-01

    Full Text Available Introduction. The relationships of the biochemical components in seminal plasma and serum, and their origins and physiological effects in male reproductive system have been poorly understood. Methods. Based on the calibration and quality control measures, 26 biochemical markers, in seminal plasma and serum samples from 36 male infertility patients with nonazoospermia were detected and compared. Results. Only PA was undetectable in all seminal plasma samples. There were significant differences of all other 24 biochemical markers in seminal plasma and serum (P<0.05 except for UA (P=0.214. There were rich proteins in seminal plasma, and globulin accounted for about 90%. There were also abundant enzymes in seminal plasma, and the activities of ALT, AST, AKP, GGT, LDH, CK, and αHBDH in seminal plasma were significantly higher than those in serum while ADA was inversely lower. There were relatively low levels of Glu, TG, TC, and hsCRP in seminal plasma, but Glu was undetectable in 8 of 36 cases. Conclusions. The differences of the levels of biochemical markers in seminal plasma and serum might be associated with the selective secretion of testis, epididymis and male accessory glands, and the specific environment needed for sperm metabolism and function maintenance.

  5. Leaf biochemical responses and fruit oil quality parameters in olive plants subjected to airborne metal pollution.

    Science.gov (United States)

    Fourati, Radhia; Scopa, Antonio; Ben Ahmed, Chedlia; Ben Abdallah, Ferjani; Terzano, Roberto; Gattullo, Concetta Eliana; Allegretta, Ignazio; Galgano, Fernanda; Caruso, Marisa Carmela; Sofo, Adriano

    2017-02-01

    This study was carried out in two olive orchards (Olea europaea L., cv. Chemlali) located in a polluted area near a fertilizers factory and in a control unpolluted site, managed with similar cultivation techniques. The aim was to investigate the physiological and biochemical responses of polluted plants (PP), exposed to atmospheric metal contamination (Cd, Cu, Fe, Mn, Ni and Pb) as compared to control plants (CP). Leaves, roots and fruits of PP showed a depression of their non-enzymatic and enzymatic antioxidant defences and a disruption of their hormonal homeostasis. The anomalous physiological status of PP was also demonstrated by the lower values of pigments in leaves and fruits, as compared to CP. Atmospheric metals negatively affected olive oil chemical and sensory quality. However, despite metal deposition on fruit surfaces, the accumulation of potentially toxic metals in olive oil was negligible. Considering that olive oil is an important food product worldwide and that many productive olive orchards are exposed to several sources of pollution, this work could contribute to clarify the effects of atmospheric metal pollution on olive oil quality and its potential toxicity for humans. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Effects of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854)

    Science.gov (United States)

    Zhu, Zhiming; Song, Bolan; Lin, Xiaotao; Xu, Zhongneng

    2016-01-01

    This study examines the effect of water-current speed on hematological, biochemical and immune parameters in juvenile tinfoil barb ( Barbonymus schwanenfeldii). Blood samples were taken on days 1, 23 and 45 from control fish and from two training groups maintained at current speeds of 0.06 bl/s (body length per second), 0.66 bl/s, and 1.92 bl/s, respectively. Significantly increased red-blood-cell counts and hematocrit were observed in the post-training groups on days 23 and 45. Significantly increased hemoglobin concentrations were also observed in the 1.92 bl/s group on days 23 and 45. In contrast, values of mean corpuscular volume were significantly lower in the 1.92 bl/s group than in the other groups on day 45. Nitroblue-tetrazolium-positive cells and lysozyme and superoxidase dismutase activities in the plasma increased significantly with increasing training intensity on days 23 and 45. Antibacterial activities were significantly increased in the trained groups compared with the control group on day 23; significantly elevated alkaline phosphatase activity was observed in the 1.92 bl/s groups on day 45. Therefore, training intensities of 0.66 and 1.92 bl/s enhanced the blood oxygen-carrying capability and plasma immune parameters of juvenile tinfoil barbs.

  7. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation

    International Nuclear Information System (INIS)

    Alcarde, Andre Ricardo

    2000-01-01

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  8. Effects of D2O on biochemical parameters of normal cells and tumour cells

    International Nuclear Information System (INIS)

    Biesewig, G.

    1975-01-01

    The influence of high temperatures (Hyperthermia) on normal tissue and Ehrlich-Ascites tumour cells ('ATZ') was examined under several conditions with regard to the application of deuterium oxide as a stabilising factor. It was proven that the DNA-synthesis of normal tissue (liver, mouse) is not sensitive to temperature. This effect of hyperthermia only occurs when the tissue is damaged, e.g. by trypsinising. The influence of hyperthermia on several biochemical parameters and on morphological changes of the Ascites cells was examined. The findings show that deuterium oxide (D 2 O) is able to reduce both the thermal and the ureal denaturation of enzymes. Thus tests were carried out to find out if D 2 O also reduces toxic influence in complicated biological systems. The assumption of high D 2 O concentrations to prevent several reactions was confirmed. When the Ascites tumour cells in the H 2 O-buffer were exposed to the damaging influence of hyperthermia, the high degree of damage was seen with the decreasing DNA synthesis, reduced aerobic glycose capacity, a drop in the ATP values and breakdown of the permeability of the membrane. Deuterium oxide was able under high temperature (from appr. 44 0 C on) to reduce the degree of damage to DNA synthesis, while auto-effects (inhibition of synthesis) of D 2 O predominate in the lower region. Aerobic glycolysis was damaged in both cases to the same degree, however. In D 2 O after hyperthermia the ATP-level dropped faster than in H 2 O. D 2 O not only reduces the thermal denaturation of the Ascites tumour cells, but it also eliminates the toxic influence of the zytostaticum TRENIMONsup(R) (under 38 0 or 46 0 C incubation). (orig./AJ) [de

  9. Knowledge and Attitude on Cardiovascular Disease Risk Factors and Their Relationship with Obesity and Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    Omid Toupchian

    2016-10-01

    Full Text Available Background: According to the high prevalence of cardiovascular diseases (CVDs in developing countries as well as high treatment expenses for patients and health-care systems, CVDs prevention in such societies has a great importance. One of the most effective strategies is improvement of knowledge and attitude towards the CVDs risk factors. This study aimed to evaluate the knowledge and attitude of females on CVDs risk factors and also the relationship between knowledge and attitude with obesity indices and biochemical parameters. Methods: This cross-sectional study was performed on 89 women with the age range of 11 to 67 y old and body mass index (BMI of 24 to 51 kg/m2. Participants were interviewed face to face using a valid questionnaire in order to evaluate their knowledge and attitude on CVDs risk factors. Blood sampling was implemented after 12 h of overnight fasting. Fasting blood glucose (FBG and lipid profile were assessed by enzymatic methods. Anthropometric measurements were performed and obesity indices were calculated. Results: More than 70% of participants had moderate or good knowledge and attitude about CVDs risk factors. Participant's knowledge and attitude had a significant positive association with educational level and a significant reverse relationship with age, total cholesterol, LDL-cholesterol, and obesity indices. Conclusions: Results of this study indicated that knowledge and attitude levels on CVDs risk factors affect the obesity indices and metabolic profile. Hence, knowledge and attitude enhancement is the main target and initial step in improving life quality and preventing cardiovascular diseases.

  10. Clinical, Toxicological, Biochemical, and Hematologic Parameters in Lead Exposed Workers of a Car Battery Industry

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    Sina Kianoush

    2013-03-01

    Full Text Available Background: Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Methods: Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto Analyzer; Model BT3000 and hematologic (using Cell Counter Sysmex; Model KX21N parameters. An atomic absorption spectrometer (Perkin-Elmer, Model 3030, USA was used to determine lead concentration in blood and urine by heated graphite atomization technique. Results: A total of 112 men mean age 28.78±5.17 years, who worked in a car battery industry were recruited in the present study. The most common signs/symptoms of lead poisoning included increased excitability 41.9%, arthralgia 41.0%, fatigue 40.1%, dental grey discoloration 44.6%, lead line 24.1%, increased deep tendon reflexes (DTR 22.3%, and decreased DTR (18.7%. Blood lead concentration (BLC was 398.95 µg/L±177.40, which was significantly correlated with duration of work (P=0.044 but not with the clinical manifestations of lead poisoning. However, BLC was significantly correlated with urine lead concentration (83.67 µg/L±49.78; r2=0.711; P<0.001, mean corpuscular hemoglobin (r=-0.280; P=0.011, mean corpuscular hemoglobin concentration (r=-0.304; P=0.006 and fasting blood sugar or FBS (r=-0.258; P=0.010. Conclusion: Neuropsychiatric and skeletal findings were common manifestations of chronic occupational lead poisoning. BLC was significantly correlated with duration of work, urine lead concentration, two hemoglobin indices and FBS.

  11. Clinical, Toxicological, Biochemical, and Hematologic Parameters in Lead Exposed Workers of a Car Battery Industry

    Science.gov (United States)

    Kianoush, Sina; Balali-Mood, Mahdi; Mousavi, Seyed Reza; Shakeri, Mohammad Taghi; Dadpour, Bita; Moradi, Valiollah; Sadeghi, Mahmoud

    2013-01-01

    Background: Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Methods: Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto Analyzer; Model BT3000) and hematologic (using Cell Counter Sysmex; Model KX21N) parameters. An atomic absorption spectrometer (Perkin-Elmer, Model 3030, USA) was used to determine lead concentration in blood and urine by heated graphite atomization technique. Results: A total of 112 men mean age 28.78±5.17 years, who worked in a car battery industry were recruited in the present study. The most common signs/symptoms of lead poisoning included increased excitability 41.9%, arthralgia 41.0%, fatigue 40.1%, dental grey discoloration 44.6%, lead line 24.1%, increased deep tendon reflexes (DTR) 22.3%, and decreased DTR (18.7%). Blood lead concentration (BLC) was 398.95 µg/L±177.40, which was significantly correlated with duration of work (P=0.044) but not with the clinical manifestations of lead poisoning. However, BLC was significantly correlated with urine lead concentration (83.67 µg/L±49.78; r2=0.711; Psugar or FBS (r=-0.258; P=0.010). Conclusion: Neuropsychiatric and skeletal findings were common manifestations of chronic occupational lead poisoning. BLC was significantly correlated with duration of work, urine lead concentration, two hemoglobin indices and FBS. PMID:23645955

  12. Clinical, toxicological, biochemical, and hematologic parameters in lead exposed workers of a car battery industry.

    Science.gov (United States)

    Kianoush, Sina; Balali-Mood, Mahdi; Mousavi, Seyed Reza; Shakeri, Mohammad Taghi; Dadpour, Bita; Moradi, Valiollah; Sadeghi, Mahmoud

    2013-03-01

    Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto Analyzer; Model BT3000) and hematologic (using Cell Counter Sysmex; Model KX21N) parameters. An atomic absorption spectrometer (Perkin-Elmer, Model 3030, USA) was used to determine lead concentration in blood and urine by heated graphite atomization technique. A total of 112 men mean age 28.78±5.17 years, who worked in a car battery industry were recruited in the present study. The most common signs/symptoms of lead poisoning included increased excitability 41.9%, arthralgia 41.0%, fatigue 40.1%, dental grey discoloration 44.6%, lead line 24.1%, increased deep tendon reflexes (DTR) 22.3%, and decreased DTR (18.7%). Blood lead concentration (BLC) was 398.95 µg/L±177.40, which was significantly correlated with duration of work (P=0.044) but not with the clinical manifestations of lead poisoning. However, BLC was significantly correlated with urine lead concentration (83.67 µg/L±49.78; r(2)=0.711; Psugar or FBS (r=-0.258; P=0.010). Neuropsychiatric and skeletal findings were common manifestations of chronic occupational lead poisoning. BLC was significantly correlated with duration of work, urine lead concentration, two hemoglobin indices and FBS.

  13. The effects of Ramadan fasting and physical activity on body composition and hematological biochemical parameters

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    Seyyed Reza Attarzadeh Hosseini

    2014-09-01

    Full Text Available Introduction: Hunger and reduction in regular energy intake can lead to a number of problems based on their intensity. For instance, low energy level can cause blood cell production to decline or it may pose a higher risk of anemia. It can also weaken the immune system and platelet aggregation or negatively affect clot formation. This study aimed to have a closer look at fasting and regular physical activity and their impacts on body composition and blood hematological-biochemical parameters among professional wrestlers. Method: In this semi-experimental study, 9 subjects were selected by convenience sampling. The selected training program included participation in this exerciseprogram, 90 min per session, 6 times per week for a period of one month. Blood samples were obtained four times: before the start of Ramadan, 2 weeks after the start, during the last week and 2 weeks after the end of Ramadan. To make intra-group comparison, repeated measure analysis of variance was used. For all statistical comparisons, the level of significance was considered at PResults: Body weight and red blood cell count (RBC dropped significantly at the end of Ramadan (Respectively P= 0.001 and P=0.034. However, the number of white blood cells (WBC and circulating platelets (PLT significantly increased during fasting (Respectively P= 0.048 and P=0.042. As a matter of fact, PLT and WBC were the only factors which dramatically increased during fasting. Intra-group variations of tetracycline (TC, low-density lipoprotein (LDL, LDL: high-density lipoprotein (HDL, triglyceride (TG: HDL and TC: HDL reduced at the end of Ramadan. However, HDL levels ​​drastically increased during fasting (P≤0.05. Conclusion: Based on the results of the research, despite being a regular activity and fasting has beneficial effects on lipid profile in athletes, however, they can with tangible changes in hematological factors may lead to weaken the immune system of athletes.

  14. Short-term effects of sibutramine on mineral status and selected biochemical parameters in obese women.

    Science.gov (United States)

    Suliburska, Joanna; Bogdański, Paweł; Szulińska, Monika; Pupek-Musialik, Danuta

    2012-11-01

    The aim of this study was to assess the effect of sibutramine on mineral status and selected biochemical parameters in obese women. The study was conducted on 24 patients who received 15 mg daily doses of sibutramine for 12 weeks, and on 20 patients who received placebo. At the baseline, after the sixth and twelfth weeks of treatment, body weight and blood pressure were measured, the BMI was calculated, and samples of blood and of first morning urine were collected. Serum lipid profiles, glucose levels, and nitric oxide levels were determined. The iron (Fe), copper (Cu), zinc (Zn), calcium (Ca), and magnesium (Mg) present in the serum and urine samples were assessed. The erythrocyte hemolysate of the patients was use to assay the activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD). No changes were observed in BMI, blood pressure, or nitric oxide during the study. After 12 weeks of treatment, a decrease was observed in total cholesterol, LDL cholesterol, triglyceride, glucose, and ferritin levels. GSH-Px and SOD activity increased after 12 weeks of sibutramine treatment. The Mg and Cu increases was observed in serum after the sixth and twelfth weeks of treatment. It was found that the Zn level decreased in serum after the twelfth week. The elimination of Ca, Mg, Fe, Zn, and Cu in urine also declined in the twelfth week. No differences were found in the women taking the placebo. In conclusion, we found that sibutramine had a positive effect on lipid and glucose status in obese women. However, the drug disturbed the balance of minerals, especially Zn and Mg, in the subjects.

  15. Biochemical and physiological parameters and estimated work output in draught horses pulling loads for long periods.

    Science.gov (United States)

    Perez, R; Recabarren, S E; Valdes, P; Hetz, E

    1992-01-01

    A study was undertaken in five draught horses of 648 +/- 33 kg body weight to find the effects of continuously pulling loads on their cardiovascular, respiratory and metabolic responses. A cart equipped with an odometer, for measuring distance, and a hydraulic dynamometer, for measuring draught force, was used. Heart and respiration rates and rectal temperatures were recorded. Blood samples for measuring arterial and venous pH and blood gases, haemoglobin, glucose and lactic acid concentrations and the serum activity of the enzymes creatine phosphokinase (CK), lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase were taken before exercise and immediately after each journey (morning and afternoon) of the daily work. Draught exercise, with loads which generated forces of between 0.57 and 0.59 kN, at speeds of 1.60 to 2.11 m/s, for 8 h daily for five consecutive days, with resting intervals of 10 min each hour, was well tolerated. Exercise tolerance was evaluated from the recovery from the changes observed in the biochemical and physiological parameters induced by the work. The analysis of these showed that, when the horses were subjected to prolonged periods of resting, their loss of fitness for work was shown by significant increases in the serum activity of muscle-derived enzymes and in blood lactate concentrations during the first day of work. However, over the following days the horses adapted to the work, so that the decreases in serum enzyme activities and blood lactate concentrations were reduced. Since similar observations have been described for racehorses, the determination of blood lactate concentrations and the serum activities of muscle-derived enzymes, specifically CK, seem to be good indicators of fitness in draught horses.

  16. Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L.

    Science.gov (United States)

    Bagheri, Mahsa; Mansouri, Hakimeh

    2015-03-01

    This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable.

  17. Pathomorphological changes of lungs and functional biochemical parameters of respiratory function in patients with intracerebral hemorrhages

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    S. I. Tertyishniy

    2013-08-01

    Full Text Available Pulmonary complications and closely related with them systemic functional-biochemical abnormalities are the most common complications of cerebral stroke. The purpose of the work was to research functional-biochemical parameters of respiratory function and to find it’s association with morphological changes in the lungs because of intracerebral hemorrhage. 37 sectional observations in patients who die of intracerebral hemorrhage were analized. It was conducted analysis of morphological transformations and retrospective analysis of functional-biochemical findings of respiratory functions, in order to detect changes of systemic hemodynamics, oxygen balance and ion-osmotic one and acid-alkaline status. The results of the study. The changes of blood gas composition and acid-alkaline status are registered in the lungs in the background of microcirculation disturbance from the first hours of development of intracerebral hemorrhage. During the first days of the onset of disease the partial oxygen pressure in arterial blood (РаО2 decreased 50,88% in comparison with the calculated normative findings. Partial pressure of carbon dioxide (РаСО2 in the arterial blood rosed by 52,17%. Without conducting artificial lung ventilation PO2 in venous blood decreased by 45,9%, and PCO2 increased by 40%. I has been marked a decrease in the oxygen capacity of the blood, due to the reduction of hemoglobin content in the blood to 103,7 minus 2 g/l and the concomitant decline in hematocrit up to 0,24-0,3. Deficit of the foundations has averaged from -6 to -10 mmol/l. These changes have led to a impression of the respiratory and metabolic acidosis, with a significant increase in the concentration of hydrogen ions. In the first day after the development of the disease with a rapid growing marks of swelling of the brain with concomitant dislocation of the brain stem, blood pH was 7,28 minus 0,02. It has been histologically determined interstitial edema, that

  18. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats

    Science.gov (United States)

    Piątkowska, Ewa; Kopeć, Aneta; Bieżanowska-Kopeć, Renata; Pysz, Mirosław; Kapusta-Duch, Joanna; Koronowicz, Aneta Agnieszka; Smoleń, Sylwester; Skoczylas, Łukasz; Ledwożyw-Smoleń, Iwona; Rakoczy, Roksana; Maślak, Edyta

    2016-01-01

    Iodine is one of the trace elements which are essential for mammalian life. The major objective of iodine biofortification of plants is to obtain food rich in this trace element, which may increase its consumption by various populations. Additionally, it may reduce the risk of iodine deficiency diseases. In this research for the first time we have assessed the bioavailability of iodine from raw or cooked carrot biofortified with this trace element on iodine concentration in selected tissues and various biochemical parameters as well as mRNA expression of some genes involved in iodine metabolism in Wistar rats. Statistically, a significantly higher iodine level was determined in urine, faeces and selected tissues of rats fed a diet containing biofortified raw carrot as compared to a diet without iodine and a diet containing control cooked carrot. Biofortified raw carrot significantly increased triiodothyronine concentration as compared to animals from other experimental groups. The highest thyroid stimulating hormone level was determined in rats fed control cooked carrots. mRNA expression of selected genes was affected by different dietary treatment in rats’ hearts. Biofortified raw and cooked carrot could be taken into account as a potential source of iodine in daily diets to prevent iodine deficiency in various populations. PMID:27043135

  19. Effects of Nano-zinc on Biochemical Parameters in Cadmium-Exposed Rats.

    Science.gov (United States)

    Hejazy, Marzie; Koohi, Mohammad Kazem

    2017-12-01

    Cadmium (Cd) is a toxic environmental and occupational pollutant with reported toxic effects on the kidneys, liver, lungs, bones, and the immunity system. Based on its physicochemical similarity to cadmium, zinc (Zn) shows protective effects against cadmium toxicity and cadmium accumulation in the body. Nano-zinc and nano-zinc oxide (ZnO), recently used in foods and pharmaceutical products, can release a great amount of Zn 2+ in their environment. This research was carried out to investigate the more potent properties of the metal zinc among sub-acute cadmium intoxicated rats. Seventy-five male Wistar rats were caged in 15 groups. Cadmium chloride (CdCl 2 ) was used in drinking water to induce cadmium toxicity. Different sizes (15, 20, and 30 nm) and doses of nano-zinc particles (3, 10, 100 mg/kg body weight [bw]) were administered solely and simultaneously with CdCl 2 (2-5 mg/kg bw) for 28 days. The experimental animals were decapitated, and the biochemical biomarkers (enzymatic and non-enzymatic) were determined in their serum after oral exposure to nano-zinc and cadmium. Statistical analysis was carried out with a one-way ANOVA and t test. P zinc-treated rats. AST, ALT, triglyceride, total cholesterol, LDL, and free fatty acids increased significantly in the cadmium- and nano-zinc-treated rats compared with the controls. However, albumin, total protein, and HDLc significantly decreased in the cadmium- and nano-zinc-treated rats compared with the controls (P zinc, the smaller sizes with low doses and the larger sizes with high doses are more toxic than metallic zinc. In a few cases, an inverse dose-dependent relationship was seen as well. This research showed that in spite of larger sizes of zinc, smaller sizes of nano-zinc particles are not suitable for protection against cadmium intoxication.

  20. Comparative Life Cycle Assessment of Lignocellulosic Ethanol Production: Biochemical Versus Thermochemical Conversion

    Science.gov (United States)

    Mu, Dongyan; Seager, Thomas; Rao, P. Suresh; Zhao, Fu

    2010-10-01

    Lignocellulosic biomass can be converted into ethanol through either biochemical or thermochemical conversion processes. Biochemical conversion involves hydrolysis and fermentation while thermochemical conversion involves gasification and catalytic synthesis. Even though these routes produce comparable amounts of ethanol and have similar energy efficiency at the plant level, little is known about their relative environmental performance from a life cycle perspective. Especially, the indirect impacts, i.e. emissions and resource consumption associated with the production of various process inputs, are largely neglected in previous studies. This article compiles material and energy flow data from process simulation models to develop life cycle inventory and compares the fossil fuel consumption, greenhouse gas emissions, and water consumption of both biomass-to-ethanol production processes. The results are presented in terms of contributions from feedstock, direct, indirect, and co-product credits for four representative biomass feedstocks i.e., wood chips, corn stover, waste paper, and wheat straw. To explore the potentials of the two conversion pathways, different technological scenarios are modeled, including current, 2012 and 2020 technology targets, as well as different production/co-production configurations. The modeling results suggest that biochemical conversion has slightly better performance on greenhouse gas emission and fossil fuel consumption, but that thermochemical conversion has significantly less direct, indirect, and life cycle water consumption. Also, if the thermochemical plant operates as a biorefinery with mixed alcohol co-products separated for chemicals, it has the potential to achieve better performance than biochemical pathway across all environmental impact categories considered due to higher co-product credits associated with chemicals being displaced. The results from this work serve as a starting point for developing full life cycle

  1. Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men

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    Meng Rao

    2015-01-01

    Full Text Available In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below, motility (P = 0.009 and 0.021, respectively, the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively, total acrosin activity (P = 0.018 and 0.009, respectively, and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively. The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031. We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

  2. Assessment of process control parameters in the biochemical methane potential of sunflower oil cake

    International Nuclear Information System (INIS)

    Raposo, F.; Borja, R.; Rincon, B.; Jimenez, A.M.

    2008-01-01

    A laboratory-scale study was conducted on the batch anaerobic digestion of sunflower oil cake (SuOC), solid waste derived from the extraction process of sunflower oil. A multi-reactor system was used to compare methane production from this waste at inoculum to substrate ratios (ISRs) of 3.0, 2.0, 1.5, 1.0, 0.8 and 0.5 (expressed as volatile solids (VS) basis). The tests were carried out at mesophilic temperature (35 deg. C) and run against a control of inoculum without substrate. The results obtained in the biochemical methane potential (BMP) tests showed that the ultimate methane yield (Y M,ult ) decreased considerably from 227±23 to 107±11 ml CH 4 at standard temperature and pressure (STP) conditions g -1 VS added when the ISR decreased from 3.0 to 0.5, showing a clear influence of the ISR on the methane yield coefficient. The biodegradability (BD) of the waste also decreased from 86% to 41% when the ISR varied from 3.0 to 0.5. A net total ammonia nitrogen (TAN) yield of 39.2 mg N g -1 VS added was obtained, and this value was not influenced by the ISRs assayed, which demonstrated the appropriate operation of the hydrolytic-acidogenic stage of the overall digestion process. A clear imbalance of the methanogenic process was observed at the lowest ISRs studied (0.5 and 0.8) due to a considerable increase in CODs and TVFA in the digestates. The profile of VFA was also influenced by the ISR, typical of the proteinaceous substrates

  3. Evaluation of tropical water sources and mollusks in southern Brazil using microbiological, biochemical, and chemical parameters.

    Science.gov (United States)

    Souza, Doris Sobral Marques; Ramos, Ana Paula Dores; Nunes, Fabrício Flores; Moresco, Vanessa; Taniguchi, Satie; Leal, Diego Averaldo Guiguet; Sasaki, Silvio Tarou; Bícego, Márcia Caruso; Montone, Rosalinda Carmela; Durigan, Maurício; Teixeira, Adriano Luiz; Pilotto, Mariana Rangel; Delfino, Nicésio; Franco, Regina Maura Bueno; Melo, Cláudio Manoel Rodrigues de; Bainy, Afonso Celso Dias; Barardi, Célia Regina Monte

    2012-02-01

    Florianópolis, a city located in the Santa Catarina State in southern Brazil, is the national leading producer of bivalve mollusks. The quality of bivalve mollusks is closely related to the sanitary conditions of surrounding waters where they are cultivated. Presently, cultivation areas receive large amounts of effluents derived mainly from treated and non-treated domestic, rural, and urban sewage. This contributes to the contamination of mollusks with trace metals, pesticides, other organic compounds, and human pathogens such as viruses, bacteria, and protozoan. The aim of this study was to perform a thorough diagnosis of the shellfish growing areas in Florianópolis, on the coast of Santa Catarina. The contamination levels of seawater, sediments, and oysters were evaluated for their microbiological, biochemical, and chemical parameters at five sea sites in Florianópolis, namely three regular oyster cultivation areas (Sites 1, 2, and oyster supplier), a polluted site (Site 3), and a heavily polluted site (Site 4). Samples were evaluated at day zero and after 14 days. Seawater and sediment samples were collected just once, at the end of the experiment. Antioxidant defenses, which may occur in contaminated environments in response to the increased production of reactive oxygen species (ROS) by organisms, were analyzed in oysters, as well as organic compounds (in oysters and sediment samples) and microbiological contamination (in oysters and seawater samples). The results showed the presence of the following contaminants: fecal coliforms in seawater samples (four sites), human adenovirus (all sites), human noroviruses GI and GII (two sites), Hepatitis A viruses (one site), JC Polyomavirus in an oyster sample from the oyster supplier, Giardia duodenalis cysts, and Cryptosporidium sp oocysts (one site). Among organochlorine pesticides, only DDT (dichlorodiphenyltrichloroethane) and HCH (hexachlorocyclohexane) were detected in some sediment and oysters samples in very

  4. Effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Ana Angélica Henrique Fernandes

    2009-02-01

    Full Text Available Amongst the numerous co-adjuvant therapies which could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of naringerin on biochemical parameters in streptozotocin-induced (STZ - 60 mg/kg, i.p. diabetic rats. Male rats were divided into four groups: G1: untreated controls; G2: normal rats receiving naringerin; G3: untreated diabetics; G4: diabetics rats receiving naringerin. The naringerin (50mg/kg, i.p, decreased the hyperglycaemia and hyperlipidaemia associated with STZ-diabetes. The concentrations of serum insulin in treated diabetic rats tended to be increased. Naringerin treatment prevents STZ-induced changes in the activities of ALT, AST and LDH in the liver and heart, indicating the protective effect of naringerin against the hepatic and cardiac toxicity caused by STZ. The glycogen level in cardiac and hepatic tissues elevated with naringerin in diabetic rats. The naringerin can improve the glucose and lipid metabolism and is beneficial in preventing diabetic complications.Dentre as numerosas terapias para minimizar as complicações diabéticas, os antioxidantes e flavonoides são testados na clínica médica. Foi analisado o efeito da naringerina sobre os parâmetros bioquímicos em ratos diabéticos induzidos por estreptozotocina (STZ - 60mg/kg, i.p.. Ratos machos foram divididos em 4 grupos: G1: controle não tratado; G2: ratos normais que receberam naringerina; G3: diabéticos não tratados; G4: ratos diabéticos que receberam naringerina. Naringerina (50mg/kg, i.p., decresceu a hiperglicemia e a hiperlipidemia em ratos diabéticos. A concentração sérica de insulina em ratos tratados tendeu aumentar. A naringerina preveniu as alterações, provocadas pela estreptozotocina, na atividade hepática e cardíaca de ALT, AST e LDH, indicando o efeito protetor da naringerina sobre estes tecidos, contra toxicidade

  5. Effect of parenterally administered cystamine and gammaphos (WR-2721) on some biochemical parameters in dog blood serum

    International Nuclear Information System (INIS)

    Simsa, J.; Tichy, M.; Podzimek, F.; Spelda, S.; Resl, M.; Kuna, P.

    1987-01-01

    The effects were studied of intravenous and intramuscular administration of radioprotectives cystamine and gammaphos in dogs on the biochemical parameters of the blood serum. The activities were studied of enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine kinase, gamma-glutamyl transpeptidase, lactate dehydrogenase, malate dehydrogenase, sorbitol dehydrogenase and alpha-amylase. The contents were determined of total protein, albumin, bilirubin, urea nitrogen, creatinine, glucose, triglycerides, cholesterol, lipids, calcium, sodium and potassium. Cystamine was shown to be hepatotoxic. The intramuscular administration of gammaphos was found to be more advantageous than of cystamine. Only slight increase was observed in the activities of lactate dehydrogenase, malate dehydrogenase, and alpha-amylase. With cystamine, the changes in all biochemical parameters were most marked. (M.D.). 17 figs., 18 refs

  6. Graded levels of sugar syrup in broiler rations and its effect on growth performance and blood biochemical parameters

    Directory of Open Access Journals (Sweden)

    Ahmed S. Hussein

    2016-09-01

    Full Text Available Dietary energy for chickens normally includes cereal grains and fat. This innovative study investigated the effect of replacing part of the corn and fat in broiler chicken rations with graded levels of sugar syrup on growth performance and biochemical parameters. Experimental treatments consisted of feeding a corn-soy basal diet alone, or with graded levels of sugar syrup in increments of 5%, 10% and 15%. All starter diets were isonitrogenous and isocaloric. Body weight gain and efficiency of feed utilization of chicks fed the control diet alone were not significantly (P < 0.05 different from chicks fed diets supplemented with either 5% or 15% sugar syrup. Supplementation of sugar syrup to broiler diets had no significant effect on blood glucose, creatinine, total protein, or liver enzymes. Adding 5% sugar syrup to broiler rations significantly decreased blood cholesterol and triglycerides in chickens fed the sugar syrup diet compared with birds fed the control diet. In conclusion, the results shows sugar syrup can be used in poultry ration to replace part of the corn as a source of energy. These results allowed the authors to recommend the safe usage of sugar syrup in broiler rations.

  7. Comparisons of blood biochemical parameters, digestive enzyme activities and volatile fatty acid profile between Meishan and Yorkshire piglets

    Directory of Open Access Journals (Sweden)

    Shouqing Ma

    2015-12-01

    Full Text Available This study was conducted to compare physiological characteristics between Meishan and Yorkshire piglets in their early lives. Six healthy purebred Meishan sows and Yorkshire sows with close farrowing dates were used in this research. The piglets sucked their respective sow's milk for 14 days, then they were slaughtered to collect samples of blood, pancreas, contents of stomach, jejunum, cecum, colon as well as feces for analysis of blood biochemical parameters, digestive enzymes, and volatile fatty acid (VFA. The results showed that Yorkshire piglets had higher concentrations of high-density lipoprotein cholesterol (HDL-C and total cholesterol (TC (P < 0.05. Gastric lipase activity was higher in Meishan piglets but Yorkshire piglets had higher lactase activity (P < 0.05. The total VFA together with acetate and propionate in cecum and colon were higher in Meishan piglets than in Yorkshire piglets (P < 0.05, but acetate in jejunum and ratio of acetate to propionate in colon were lower in Meishan piglets than in Yorkshire piglets (P < 0.05. In conclusion, in early suckling period, significant differences exist in host metabolism and intestinal microbial metabolism between Meishan and Yorkshire piglets.

  8. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation.

    Science.gov (United States)

    Mishra, Arun K; Mishra, Amrita; Verma, Anurag; Chattopadhyay, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with special reference to 1,8-cineole and α-pinene. The results indicated that the treatment with creams containing 4% and 5% of Calendula essential oil caused a significant decrease in the malonyldialdehyde level, whereas the levels of catalase, glutathione, superoxide dismutase, ascorbic acid, and the total protein level were significantly increased after 1 month of daily irradiation and treatment when compared to untreated control groups. The results suggest that the cutaneous application of the essential oil of Calendula prevents UV-B-induced alterations in the level of antioxidants in skin tissue.

  9. Dietary supplementation of tiger nut alters biochemical parameters relevant to erectile function in l-NAME treated rats.

    Science.gov (United States)

    Olabiyi, Ayodeji A; Carvalho, Fabiano B; Bottari, Nathieli B; Lopes, Thauan F; da Costa, Pauline; Stefanelo, Naiara; Morsch, Vera M; Akindahunsi, Afolabi A; Oboh, Ganiyu; Schetinger, Maria Rosa

    2018-07-01

    Tiger nut tubers have been reportedly used for the treatment of erectile dysfunction (ED) in folk medicine without scientific basis. Hence, this study evaluated the effect of tiger nut on erectile dysfunction by assessing biochemical parameters relevant to ED in male rats by nitric oxide synthase (NOS) inhibitor, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME) treatment. Rats were divided into five groups (n = 10) each: Control group; l-NAME plus basal diet; l-NAME plus Sildenafil citrate; diet supplemented processed tiger nut (20%) plus l-NAME;diet supplemented raw tiger nut (20%) plus l-NAME. l-NAME pre-treatment (40 mg/kg/day) lasted for 14 days. Arginase, acetycholinesterase (AChE) and adenosine deaminase (ADA) activities as well as nitric oxide levels (NO) in serum, brain and penile tissue were measured. l-NAME increased the activity of arginase, AChE and ADA and reduced NO levels. However, dietary supplementation with tiger nut caused a reduction on the activities of the above enzymes and up regulated nitric oxide levels when compared to the control group. The effect of tiger nut supplemented diet may be said to prevent alterations of the activities of the enzymes relevant in erectile function. Quercetin was revealed to be the most active component of tiger nut tuber by HPLC finger printing. Copyright © 2018. Published by Elsevier Ltd.

  10. Effect of Dietary Cysteine Supplementation on Growing Performance, Pelt Quality and Some Serum Biochemical Parameters of Young Rex Rabbit

    OpenAIRE

    Tossou Myrlene Carine B; Han Xu-feng; Chen Yu-Lin

    2013-01-01

    The present study was conducted to evaluate the effects of dietary cysteine, a sulphur containing amino acid supplementation on growth performance, pelt quality and a number of serum biochemical parameters of young Rex Rabbit. One hundred and twenty Rex Rabbits aged 45 days were divided into five dietary treatment groups including one control group and 4 experimental groups. Each group was composed of 24 animals and was fed with different diets for 56 days corresponding to the fattening peri...

  11. Effects of Calendula Essential Oil-Based Cream on Biochemical Parameters of Skin of Albino Rats against Ultraviolet B Radiation

    OpenAIRE

    MISHRA, Arun; MISHRA, Amrita; VERMA, Anurag; CHATTOPADHYAY, Pronobesh

    2012-01-01

    Reactive oxygen species (ROS) generated from UV-B radiation have the capacity to cause oxidative decomposition which leads to the formation of toxic components as well as lipid peroxidation. Considering this fact, the present study was performed to evaluate the effect of a cream (O/W) containing the essential oil of Calendula officinalis on biochemical parameters of the skin of albino rats against UV-B radiation. The fingerprint analysis of Calendula essential oil was performed by HPLC with s...

  12. The changes in biochemical parameters due to wine consumption depending on gender

    Directory of Open Access Journals (Sweden)

    Martina Gažarová

    2016-10-01

    Full Text Available The aim of this study was to investigate the effect of red wine consumption on the lipid profile and glucose in the group of male (13 men aged 34 - 64 years and the group of female (11 women aged 28 - 57 years. Research consisted of moderate red wine consumption for 6 weeks. The dose of alcohol ranged from 200 to 300 mL per day of red wine Lemberger (Winery Masaryk, Slovakia. The blood samples were obtained after overnight fasting and were collected at baseline and after three days, three weeks and six weeks of wine consumption. Differences between male and female subjects were reflected in the results of different biochemical parameters in the dynamics of wine consumption. We found out that while in females the total cholesterol level did not change significantly and had a predominantly downward trend, for male subjects we observed at the beginning the slight increase of the levels, which, however, after 6 weeks of consumption significantly decreased from an initial value of 5.75 ±1.32 mmol.L-1 to 5.35 ±1.25 mmol.L-1 (p <0.05. The blood concentration of triglycerides in the dynamics of the experiment did not change significantly in either one gender, although small differences were observed, because while the female subjects had triglyceride development over consumption upward trend in male subjects it was vice versa. LDL-cholesterol changed significantly only in the group of female. Level of this lipid parameter decreased significantly during the six weeks of consumption of Lemberger from an initial value 3.37 ±0.68 mmol.L-1 to the lowest 2.99 ±0.61 mmol.L-1, which was recorded in the third week of consumption (p <0.0001, but statistically significant differences versus baseline we monitored after three days and six weeks of consumption (p <0.01. In the group of male, we did not observe such significant changes, but it should be noted, that the men had changes in LDL-cholesterol downward direction and all the values were in the range of

  13. Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758).

    Science.gov (United States)

    Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mallesh, B; Mohanty, Snatashree; Kumar, Abhay

    2013-08-01

    Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1-5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L(-1) as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2% DMSO solution without azadirachtin and considered as negative control T0(-). Along with six treatment groups, a positive control T0(+) of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L(-1) and that also shifted haematological and serum biochemical parameters towards homeostasis.

  14. Biochemical parameters of bone metabolism in bone metastases of solid tumors (Review)

    NARCIS (Netherlands)

    Meijer, Wilhelmus; van der Veer, E; Willemse, P H

    1998-01-01

    The role of biochemical markers of bone metabolism in the diagnosis and monitoring of bone metastases in solid tumors is reviewed. Emphasis is on the recently developed markers, which may provide a more accurate quantitation of bone metabolism. In metastatic bone disease, bone formation and

  15. Prematurity and Related Biochemical Outcomes: Study of Bone Mineralization and Renal Function Parameters in Preterm Infants

    Directory of Open Access Journals (Sweden)

    Sarika Singh Chauhan

    2011-01-01

    phosphorus levels were found to be significantly decreased, and serum ALP, creatinine, and electrolytes were found to be significantly increased (<0.001 at 28–30 weeks as compared to controls, but serum calcium and phosphorous levels were found to be insignificantly decreased, whereas serum ALP activities were found to be insignificantly increased at 28–30 weeks as compared to 30–32 weeks of gestational age in preterm babies. It can be concluded that high serum ALP activity and low serum calcium and phosphorus levels are associated with preterm babies. A significant difference in the mean values of these renal function parameters was also obtained, except for serum sodium and potassium.

  16. Interrelationship between Mini-Mental State Examination scores and biochemical parameters in patients with mild cognitive impairment and Alzheimer's disease.

    Science.gov (United States)

    Vasantharekha, Ramasamy; Priyanka, Hannah P; Swarnalingam, Thangavel; Srinivasan, Avathvadi Venkatesan; ThyagaRajan, Srinivasan

    2017-10-01

    The aim of the present study was to provide first-hand information about the prevalence of mild cognitive impairment (MCI) and Alzheimer's disease (AD) in Tamil Nadu, a southern state in India, and examine if there exists a relationship between cognitive functions and biochemical parameters in these patients. Surveys were collected from adults, older men and women (n = 3126) from different regions of Tamil Nadu, which were followed up after 12 months for 1337 participants. Mini-Mental State Examination (MMSE) scores, lipid profile, and liver function tests were carried out in the elderly, MCI and AD patients. Based on the MMSE scores, the elderly population was classified into old control (28.97 ± 1.49; n = 1868), MCI (19.58 ± 1.17; n = 734) and AD (7.18 ± 1.38; n = 304) groups. Peripheral blood samples were collected after overnight fast from both male and female volunteers (n = 40 per group) who were categorized as young adult control, old control, MCI and AD. AD patients showed lower MMSE scores compared with the young adults, old and MCI groups, and MMSE further decreased at follow-up examination a year later. In the serum of AD patients, high-density lipoprotein, alkaline phosphatase activity and bilirubin levels were lower, whereas low-density lipoprotein, total cholesterol and triglycerides levels were higher. MMSE was positively correlated with high-density lipoprotein, and negatively correlated with other lipid parameters in AD. Hypercholesterolemia is a risk factor for AD that might result in neurotoxicity and cognitive impairment. Dysfunction of lipoprotein and heme metabolism might also provide additional targets for AD diagnosis. Geriatr Gerontol Int 2017; 17: 1737-1745. © 2016 Japan Geriatrics Society.

  17. Biochemical parameters as monitoring markers of the inflammatory reaction by patients with chronic obstructive pulmonary disease (COPD)

    Science.gov (United States)

    Lenártová, Petra; Kopčeková, Jana; Gažarová, Martina; Mrázová, Jana; Wyka, Joanna

    Chronic obstructive pulmonary disease (COPD) is an airway inflammatory disease caused by inhalation of toxic particles, mainly cigarette smoking, and now is accepted as a disease associated with systemic characteristics. The aim of this work was to investigate and compare selected biochemical parameters in patients with and without COPD. Observation group consisted of clinically stable patients with COPD (n = 60). The control group was healthy persons from the general population, without COPD, who were divided into two subgroups – smokers (n = 30) and non-smokers (n = 30). Laboratory parameters were investigated by automated clinical chemistry analyzer LISA 200th. Albumin in our measurements showed an average value of 39.55 g.l-1 in the patient population; 38.89 g.l-1 in smokers and in non-smokers group 44.65 g.l-1. The average value of pre-albumin in the group of patients was 0.28 ± 0.28 g.l-1 and 0.30 ± 0.04 g.l-1 in smokers group. The average value of the orosomucoid in patients was about 1.11 ± 0.90 mg.ml-1. In the group of smokers, the mean value of orosomucoid was 0.60 ± 0.13 mg.ml-1. The level of C-reactive protein (CRP) in the patient group reached an average value of 15.31 ± 22.04 mg.l-1, in the group of smokers was 5.18 ± 4.58 mg. l-1. Prognostic inflammatory and nutritional index (PINI) in the group of patients showed a mean value of 4.65 ± 10.77 and 0.026 ± 0.025 in smokers. The results of this work show, that the values of index PINI in COPD patients are significantly higher than in smokers (P COPD.

  18. Serum levels of oestrogen and some biochemical parameters in relation to reproductive activities and level of production in fowl

    International Nuclear Information System (INIS)

    El - Abiad, N.M.F.

    1982-01-01

    This work was carried out at the poultry experiment station department of animal production, faculty of agriculture zagazig university, benha branch. radioimmunological and biochemical assays were performed in the laboratories of radioimmunology and biochemistry unit of atomic energy establishment . It was intended to study: 1. The effect of oestrogen level along the time of egg formation on some parameters in blood serum related to metabolic efficiency. 2. The variations in oestrogen level and blood serum parameters due to clutch size of hens. 3.Changes in oestrogen levels and some blood constituents along the time of egg formation

  19. Serum levels of progesterone and some biochemical parameters in relation to productive activities and level of production in fowl

    International Nuclear Information System (INIS)

    EL-Sayed, S.M.A.

    1982-01-01

    The purpose of this study was: 1- estimation of progesterone, thyroxine, triiodothyronine levels and some biochemical parameters in blood serum of fayoumi and rhode island red laying pullets during the time of egg formation. 2 - The variation in progesterone, thyroxine, triiodo-thyronine levels and some blood serum parameters in fayoumi and RIR breeds due to the different clutch size. 3- The relationship between serum progesterone levels and the thyroxine, triiodothyronine, total protein, albumin, globulin, calcium, inorganic phosphorus and cholesterol levels at ovulation time in fayoumi and RIR pullets

  20. A Comparative Study of Distribution System Parameter Estimation Methods

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Yannan; Williams, Tess L.; Gourisetti, Sri Nikhil Gup

    2016-07-17

    In this paper, we compare two parameter estimation methods for distribution systems: residual sensitivity analysis and state-vector augmentation with a Kalman filter. These two methods were originally proposed for transmission systems, and are still the most commonly used methods for parameter estimation. Distribution systems have much lower measurement redundancy than transmission systems. Therefore, estimating parameters is much more difficult. To increase the robustness of parameter estimation, the two methods are applied with combined measurement snapshots (measurement sets taken at different points in time), so that the redundancy for computing the parameter values is increased. The advantages and disadvantages of both methods are discussed. The results of this paper show that state-vector augmentation is a better approach for parameter estimation in distribution systems. Simulation studies are done on a modified version of IEEE 13-Node Test Feeder with varying levels of measurement noise and non-zero error in the other system model parameters.

  1. Meta-analysis of the relationship of mycotoxins with biochemical and hematological parameters in broilers.

    Science.gov (United States)

    Andretta, I; Kipper, M; Lehnen, C R; Lovatto, P A

    2012-02-01

    A meta-analysis was carried out to study the association of mycotoxins with hematological and biochemical profiles in broilers. Ninety-eight articles published between 1980 and 2009 were used in the database, totaling 37,371 broilers. The information was selected from the Materials and Methods and Results sections in the selected articles and then tabulated in a database. Meta-analysis followed 3 sequential analyses: graphic, correlation, and variance-covariance. Mycotoxins reduced (P Mycotoxins also altered (P effect was observed on the relationship between the concentration of aflatoxin in diets and the serum concentration of alkaline phosphatase, γ-glutamyl transferase, alanine aminotransferase, and aspartate aminotransferase. The total protein concentration in blood was 18% lower (P mycotoxin and without the additive. The meta-analysis performed in this study allowed us to address and quantify systematically the relationship of mycotoxins with alterations in hematologic and biochemical profiles in broilers.

  2. Investigation of Biochemical and Hematological Parameters of Workers Exposed to Arsenic

    OpenAIRE

    Bayrakçeken, Yusuf; Peker, Seydi Ali; Gündüzöz, Meşide

    2016-01-01

    Arsenic is a naturally occurring element widely distributed in the earth's crust and its toxicity is a global health problem affecting many millions of people. Contamination is caused by arsenic from natural geological sources leaching into aquifers, contaminating drinking water and may also occur from mining and other industrial processes. In this study, it was aimed to assess retrospectively the biochemical and hematological markers of workers who exposed to arsenic and referred to our...

  3. Clinical, Toxicological, Biochemical, and Hematologic Parameters in Lead Exposed Workers of a Car Battery Industry

    OpenAIRE

    Kianoush, Sina; Balali-Mood, Mahdi; Mousavi, Seyed Reza; Shakeri, Mohammad Taghi; Dadpour, Bita; Moradi, Valiollah; Sadeghi, Mahmoud

    2013-01-01

    Background: Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. Methods: Questionnaires and forms were designed and used to record demographic data, past medical histories and clinical manifestations of lead poisoning. Blood samples were taken to determine biochemical (using Auto...

  4. Effect of bioactive compounds extracted from euphorbious plants on hematological and biochemical parameters of Channa punctatus

    Directory of Open Access Journals (Sweden)

    Jaya Shahi

    2011-10-01

    Full Text Available Channa punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC50 of 24h of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration. The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC, mean corpuscular hemoglobin (MCH and mean corpuscular volume (MCV were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.

  5. Estimating Biochemical Parameters of Tea (camellia Sinensis (L.)) Using Hyperspectral Techniques

    Science.gov (United States)

    Bian, M.; Skidmore, A. K.; Schlerf, M.; Liu, Y.; Wang, T.

    2012-07-01

    Tea (Camellia Sinensis (L.)) is an important economic crop and the market price of tea depends largely on its quality. This research aims to explore the potential of hyperspectral remote sensing on predicting the concentration of biochemical components, namely total tea polyphenols, as indicators of tea quality at canopy scale. Experiments were carried out for tea plants growing in the field and greenhouse. Partial least squares regression (PLSR), which has proven to be the one of the most successful empirical approach, was performed to establish the relationship between reflectance and biochemical concentration across six tea varieties in the field. Moreover, a novel integrated approach involving successive projections algorithms as band selection method and neural networks was developed and applied to detect the concentration of total tea polyphenols for one tea variety, in order to explore and model complex nonlinearity relationships between independent (wavebands) and dependent (biochemicals) variables. The good prediction accuracies (r2 > 0.8 and relative RMSEP < 10 %) achieved for tea plants using both linear (partial lease squares regress) and nonlinear (artificial neural networks) modelling approaches in this study demonstrates the feasibility of using airborne and spaceborne sensors to cover wide areas of tea plantation for in situ monitoring of tea quality cheaply and rapidly.

  6. Influence of probiotic, Lactobacillus plantarum on serum biochemical and immune parameters in vaccinated rainbow trout (Oncorhynchus mykiss against streptococcosis/lactococosis

    Directory of Open Access Journals (Sweden)

    Ali. M. Kane

    2016-10-01

    Full Text Available This study evaluated the effects of probiotic, Lactobacillus plantarum on serum biochemical and some immune parameters of immunized rainbow trout weighting 29.6±1.84 g, with streptococcosis/lactococosis vaccine at 16±1.5°C, for 60 days. A commercial diet was used as the control. Fish in the first treatment were immunized with streptococcosis/lactococosis vaccine in bathing route for 1 min. In the second group, the vaccinated trout were also fed diet containing L. plantarum (108 CFU g-1. In the third treatment, fish were only fed the diet supplemented with L. plantarum (108 CFU g-1. The results showed that vaccinated trout with or without L. plantarum feeding diets significantly decreased heterophils. Meanwhile it enhances serum lysozyme, alternative complement activities, antibody titer, total leukocytes, lymphocytes, and serum biochemical parameters, including ALP, IgM, and total protein levels compared to control groups. Moreover, the highest levels of above mentioned parameters were found in vaccinated fish that fed L. plantarum. In addition, the vaccinated fish that fed L. plantarum showed significantly elevated cholesterol levels compared to the control group. The results showed that the dietary L. plantarum improved the immunity of immunized trout with streptococcosis/lactococosis vaccine.

  7. Effect of dietary supplementation of fermented fish silage on serum biochemical parameters of broiler Japanese quails (Coturnix coturnix japonica

    Directory of Open Access Journals (Sweden)

    Laxman Kumar Babu

    2017-04-01

    Full Text Available Aim: The objective of this study was to evaluate the effect of feeding fermented fish silage (FFS on serum biochemical parameters of Japanese quails (Coturnix coturnix japonica. Materials and Methods: A total of 192, 7-day-old broiler Japanese quail chicks of either sex, were randomly distributed into four dietary treatments with four replicates in each group having 12 chicks in each replicate pen. The dietary treatments were T1 - Control diet, T2 - Diet containing 5% FFS, T3 - Diet containing 10% FFS, and T4 - Diet containing 15% FFS. Group body weight and feed consumption were recorded at weekly intervals. Feed conversion ratio (FCR was derived by dividing the feed consumed with the weekly body weight gain. At the end of the experiment, 8 birds from each treatment were selected randomly and sacrificed by cervical dislocation to study the carcass traits expressed as % pre-slaughter live weight. At 5 weeks of age, about 2 ml of blood was taken from the jugular vein of each selected bird, and serum samples were separated after centrifugation. Total protein, albumin/globulin (A/G ratio, calcium, phosphorus, triglyceride, total cholesterol, high density lipoprotein-cholesterol (HDL-C, very low density lipoprotein-cholesterol (VLDL-C, low density lipoprotein-cholesterol (LDL-C, alanine aminotransferase (ALT, and aspartate aminotransferase (AST were estimated in the serum. Results: The cumulative body weight gains from 1 to 5 weeks of age did not vary significantly between control and 5% FFS group. However, a linear decrease in body weight gain was observed by increasing the levels of FFS beyond 5% in the diet. The cumulative feed consumption was significantly higher in case of birds under control group during 1-5 weeks of age as compared to birds fed FFS based diet (5%, 10%, and 15%. No statistical difference in cumulative feed conversion ratio could be noticed during 1-5 weeks of age. The eviscerated yield decreased and giblet weight increased due

  8. Tension cost correlates with mechanical and biochemical parameters in different myocardial contractility conditions

    Directory of Open Access Journals (Sweden)

    Cleci M. Moreira

    2012-01-01

    Full Text Available OBJECTIVES: Tension cost, the ratio of myosin ATPase activity to tension, reflects the economy of tension development in the myocardium. To evaluate the mechanical advantage represented by the tension cost, we studied papillary muscle contractility and the activity of myosin ATPase in the left ventricles in normal and pathophysiological conditions. METHODS: Experimental protocols were performed using rat left ventricles from: (1 streptozotocin-induced diabetic and control Wistar rats; (2 N-nitro-L-arginine methyl ester (L-NAME hypertensive and untreated Wistar rats; (3 deoxycorticosterone acetate (DOCA salt-treated, nephrectomized and salt- and DOCA-treated rats; (4 spontaneous hypertensive rats (SHR and Wistar Kyoto (WKY rats; (5 rats with myocardial infarction and shamoperated rats. The isometric force, tetanic tension, and the activity of myosin ATPase were measured. RESULTS: The results obtained from infarcted, diabetic, and deoxycorticosterone acetate-salt-treated rats showed reductions in twitch and tetanic tension compared to the control and sham-operated groups. Twitch and tetanic tension increased in the N-nitro-L-arginine methyl ester-treated rats compared with the Wistar rats. Myosin ATPase activity was depressed in the infarcted, diabetic, and deoxycorticosterone acetate salt-treated rats compared with control and sham-operated rats and was increased in N-nitro-L-arginine methyl ester-treated rats. These parameters did not differ between SHR and WKY rats. In the studied conditions (e.g., post-myocardial infarction, deoxycorticosterone acetate salt-induced hypertension, chronic N-nitro-L-arginine methyl ester treatment, and streptozotocin-induced diabetes, a positive correlation between force or plateau tetanic tension and myosin ATPase activity was observed. CONCLUSION: Our results suggest that the myocardium adapts to force generation by increasing or reducing the tension cost to maintain myocardial contractility with a better

  9. Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.).

    Science.gov (United States)

    Velisek, J; Svobodova, Z; Machova, J

    2009-11-01

    The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 microg l(-1). Examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to Talstar EC 10 (57.5 microg l(-1)). The experimental group showed significantly higher (P bifenthrin-based Talstar EC 10 pesticide preparation was classified as a substance strongly toxic for fish.

  10. Risks of a fructose rich soft drink consumption on some biochemical parameters in Balb/c mice

    International Nuclear Information System (INIS)

    Abd Elmonem, H.A.; Ali, E.A.

    2011-01-01

    Consumption of soft drinks, in particular carbonated beverages, has markedly increased in the last two to three decades. In fact, the carbonated beverages are the most popular refreshments among most of the world's population. The aim of this study was to assess the body weight gains and analyze some biochemical and hematological parameters in male and female Balb/c mice. Animals were provided with diluted coca cola (fructose rich soft drink) for 6 weeks and were matched against corresponding control animals. The control animals were maintained on tap water ad libitum and coca cola was diluted v/v by tap water. The results revealed that the percentages of body weight gains of male and female control animals were significantly higher than the corresponding mice provided with coca cola. Hemoglobin content (Hb, g/100ml blood), erythrocytes (RBCs), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), leucocytes (WBCs), granulocytes and blood platelet counts were significantly decreased in the groups of mice treated with the soft drink, compared to the control groups. However, significant increases were observed in lymphocytes in the treated groups. Consumption of the soft drink (coca cola) in both male and female animal groups exhibited significant increases of total cholesterol, triglycerides, low density lipoprotein-c and atherogenic index. Levels of serum calcium significantly decreased in both male and female treated mice than the corresponding control groups. Consumption of coca cola did not affect the levels of T3. This study suggests that consumption of high fructose soft drink may lead to many health problems

  11. Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis

    Directory of Open Access Journals (Sweden)

    Vinícius Paula Venâncio

    2013-08-01

    Full Text Available A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.

  12. Correlation between Aminotransferase Ratio (AST/ALT and Other Biochemical Parameters in Chronic Liver Disease of Viral Origin

    Directory of Open Access Journals (Sweden)

    Shah Md Fazlul Karim

    2015-03-01

    Full Text Available Background: In recent years the ratio of aspartate aminotransferase (AST to alanine aminotransferase (ALT in patients of chronic liver disease (CLD of various origins has gained much attention. This variable is readily available, easy to interpret, and inexpensive and the clinical utility of the AST/ALT ratio in the diagnostic workup of patients with CLD is quite promising. Objective: The present study was designed to find out the link between aminotransferase (AST/ALT ratio with commonly measured biochemical parameters of liver function tests in CLD of viral origin. Materials and method: This cross sectional study was carried out in the department of Biochemistry, Sir Salimullah Medical College, Dhaka, Bangladesh. Forty four biopsy proven diagnosed subjects of chronic viral hepatitis without cirrhosis of both sex were selected purposively. With aseptic precaution 5 mL venous blood was collected from each subject and common liver function tests (serum AST, ALT, AST/ALT ratio, alkaline phosphatase, total bilirubin, serum total protein, serum albumin, serum globulin, serum albumin/globulin ratio, prothrombin time and viral serology (HBsAg, Anti HDV antibody, Anti HCV antibody were performed. Data were analyzed by SPSS version 19 for Windows. Pearson’s correlation test was done to determine association between AST/ALT with other biochemical parameters. Results: Mean(±SD age of the study subjects was 32.55±10.55 years (range 20-50 years with 48 (77.7% male and 14 (22.6% female subjects. Pearson’s correlation test was done between AST to ALT ratio with other biochemical parameters and prothrombin time showed significant positive correlation (p <0.01. Conclusion: In our study we found significant positive correlation between AST/ALT with prothrombin time in CLD subjects without cirrhosis.

  13. Effects of dietary administration of yarrow extract on growth performance and blood biochemical parameters of rainbow trout (Oncorhynchus mykiss

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    Mahmoud Nafisi Bahabadi

    2014-10-01

    Full Text Available The present study was conducted to investigate the clinical effects and possible side effects of yarrow extract (Achillea millefolium L. as feed additive on biochemical blood parameters and growth performance of rainbow trout (Oncorhynchus mykiss. Fishes were treated with 0 (control, 0.1, 0.5 and 1% of yarrow extract for 30 days. Plasma alanine aminotransferase (ALT, aspartate aminotransferase (AST, lactate dehydrogenase (LDH, alkaline phosphatase (ALP, creatine kinase (CK, peroxidase activity, total complement and lysozyme activity, glucose, total protein, triglyceride and cholesterol were measured after 15 and 30 days of yarrow treatment. There were no significant changes in the lysozyme activity and glucose levels. Total protein and globulin levels were significantly higher in the fish fed with diets enriched with 1% yarrow extract on day 30. Triglyceride and cholesterol levels was significantly decreased in the fish fed with diets containing 0.5% and 1% yarrow extract on day 30 (P<0.05. LDH, CK and peroxidase activities in the fish fed with diets having 1% yarrow extract were significantly decreased at the end of the experiment (P<0.05. In contrast, a significant increase in AST, ALP and total complement activity was observed in the fish fed with 1% yarrow extract diet, on day 15 (P<0.05. The weight gain and specific growth rate increased and food conversion ratio decreased in in the fish fed 1% yarrow extract on day 30. Condition factor in the fish fed with yarrow extract was significantly higher than control group on 30 day. In conclusion, on the basis of these results, oral administration of yarrow extract up to 0.5% have not side effect on blood biochemical and clinical parameters of fishes. However, oral administration of 1% of yarrow extract caused cytotoxicity and modifications in blood biochemical parameters of fish.

  14. Effects of magnesium on erythrocyte sodium-lithium countertransport and some of plasma biochemical parameters in rabbit

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    Samad Akbarzadeh

    2009-02-01

    Full Text Available Background: Magnesium acts as an essential cofactor for the activity of many enzymes. It regulates the work of cardiovascular system. The activity of sodium–lithium countertransport (SLC and the concentrations of plasma biochemical parameters such as VLDL, LDL-cholesterol, HDL-cholesterol, sodium, potassium, urea and creatinine are changed in cardiovascular diseases. The aim of this study was to determine the effects of magnesium on SLC activity and some of the plasma biochemical parameters. Methods: New Zealand white rabbits (weighed 1350 ± 50g were chosen for these experiments. This study was conducted through two in vitro and in vivo techniques. Through in vitro method, the effects of different concentrations of magnesium on SLC activity were investigated. In order to conduct in vivo method, the rabbits were divided into two groups (5 /group. One group was treated by MgSo4 (40 mg/kg body weight through peritoneum for two weeks. For the second group, deionized water was used. The activity of SLC and mentioned biochemical parameters were determined. Results: The results of both in vitro and in vivo studies showed that magnesium can significantly decrease the SLC activity and also causes an increase in Km and decreased Vmax/Km of the system and plasma concentrations of VLDL, LDL-cholesterol, total cholesterol and triglycerides were significantly decreased. Conclusion: Magnesium may cause a reduction in blood pressure through decreasing the SLC activity and affecting the concentrations of VLDL, LDL-cholesterol, total cholesterol and triglycerides and so improvement the cardiovascular diseases.

  15. Phenozan Influence on the Physiological-Biochemical Parameters of the Young Minks Leading to Their Advanced Properties

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    Nikolai A. Balakirev

    2016-01-01

    Full Text Available The antioxidant influence on the physiological-biochemical parameters of productive animals is one of the most important directions in modern animal science. Phenozan is a synthetic antioxidant with high biological activities including those for animals. The positive effects of phenozan on the physiological-biochemical parameters of the mink blood were found. These effects are leading to the intensive growth, strong health, enhancement of the mink fur quality, and so forth. The total amount of the erythrocytes in the mink blood increased by about 4-5% due to phenozan inclusion in the mink diet. Even a more significant increase was found in hemoglobin content in the mink blood and erythrocytes (by about 9.5% and 8.8%, resp. due to phenozan inclusion of 100 mg/day, whereas it was found only by about 6.1–5.3% or 5.5–0.3%, owing to phenozan inclusion of 50 or 150 mg/day. For many other parameters the optimal phenozan concentration was about 100 mg/day per head according to the result obtained for all studied animal groups. Thus, phenozan can be successfully used as a biologically active compound for mink nutrition and is recommended for other farm animals.

  16. Age and sex specific variation in hematological and serum biochemical parameters of Beluga (Huso huso Linnaeus, 1758

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    Reza Akrami

    2013-05-01

    Full Text Available In the present study, the age- and sex-specific changes of various haematological and blood serum biochemical blood parameters of Beluga (Huso huso were investigated. Blood samples were collected from 4-, 6-, 7-, and 8-year-old beluga (n = 7 for each sex and age. The specimens were fed at a rate of 0.5-3% body weight per day. AST and LDH levels in 7- and 8-year-old fish of both sexes were significantly higher (P<0.05 than those in 4- and 6-year-old individuals. The mean ALT were significantly different (P<0.05 in both sexes of 4-, 6-, and 7-year-old sturgeon. However, the 6-, 7-, and 8-year-old female sturgeon had higher ALP levels (P<0.05. Additionally, mean RBC, PCV, and Hb values were significantly higher (P<0.05 in 7- and 8-year-old females and males than the others. Two-tailed Pearson’s correlation between the biochemical and haematological parameters obtained for beluga sturgeon indicated significant positive correlations between AST and ALP, AST and LDH, ALP and LDH, RBC and Hb, RBC and PCV, Hb and PCV, MCH and MCHC, and MCV and MCH. However, significant negative correlations were found between RBC and MCV and MCH. These results suggest that the blood parameters of beluga are influenced by age- and sex-specific factors.

  17. Changes in quality and biochemical parameters in 'Idared' apples during prolonged shelf life and 1-MCP treatment.

    Science.gov (United States)

    Bizjak, Jan; Slatnar, Ana; Stampar, Franci; Veberic, Robert

    2012-12-01

    In this study, changes in quality and various biochemical parameters of 'Idared' apples during prolonged shelf life period after ultra-low oxygen (ULO) storage were investigated. Additionally, the impact of the postharvest application of 1-methylcyclopropene (1-MCP) on different parameters was evaluated. After the harvest, apples were stored in the ULO storage for 6 months and then exposed to room temperature. Fruit firmness, peel color, and changes in sugars, organic acids and phenolics were monitored during the 3 weeks of shelf life. Malic acid, sugars and firmness decreased at room temperature. However, the color of the apples remained unchanged. The level of citric and ascorbic acid remained constant. Levels of phenolics in the peel increased significantly, whereas remained constant in the pulp of apples. 1-MCP treatment resulted in higher amounts of fructose and glucose, malic acid and greater firmness of apples. However, 1-MCP did not influence the phenolic content, ascorbic acid or color. The results obtained indicate that the content of different health-promoting compounds of apples does not change dramatically at room temperature. At the same time these results suggest that 1-MCP could be useful for maintaining certain quality and biochemical parameters and might extend the shelf life of apples.

  18. Biochemical parameters in the blood of grass snakes (Natrix natrix in ecosystems under varying degrees of anthropogenic influence

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    V. Y. Gasso

    2016-09-01

    Full Text Available The grass snake Natrix natrix (Linnaeus, 1758 is a partly hygrophilous species, distributed throughoutUkraine. This snake may be considered as a test object for environmental biomonitoring. Modern biochemical methods make it possible to obtain new scientific data on the effects of anthropogenic pressure on reptiles. Blood is a sensitive and informative indicator of the condition of an organism as it responds quickly to most changes in exogenous and endogenous factors, and reflects negative influences on both individual and, indirectly, populations. Changes in biochemical parameters may be used as biomarkers of the state of health of reptiles in ecosystems under varying degrees of anthropogenic pressure. Due the increase in anthropogenic influence the development and introduction of new methods of perceptual research, collection of up-to-date information and development of a database of reptile biochemical parameters have become an urgent priority. We collected mature individuals of the grass snake in floodplain ecosystems on the right bank of the Dnieper River in Dnipropetrovsk city. Grass snakes from floodplain habitats on the left bank of theSamaraRiver (O.L. Belgard Prysamarskii International Biosphere Station, Novomoskovsk district, Dnipropetrovsk province were studied as the control specimens. Our study demonstrated statistically significant differences between snakes from the study sites in the amount of albumin, urea and urea nitrogen, and inorganic phosphorus, as well as in alanine aminotransferase (ALT and alkaline phosphatise (AP activity. The amount of albumin in the blood serum of specimens from the anthropogenically transformed areas was significantly lower (by 25% than in that of the snakes caught in the control habitats. Decrease of the albumin concentration usually indicates abnormal processes in the kidneys and liver. According to the changes observed in the concentration of albumin, a corresponding increase in the albumin to

  19. Influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals

    International Nuclear Information System (INIS)

    Svambaev, Z.A.; Svambaev, E.A.; Sultanbekov, G.A; Tusupbekova, S.T.

    2010-01-01

    In the work authors inform results on studying influence of a productive solution of uranium on some biochemical parameters of blood of an organism at experimental animals. It is established that all samples of a productive solution of uranium possesses high toxicity and causes destruction of experimental animals with infringement of a picture of blood. Experiments on influence on biochemical and hematology defined parameters of blood on chickens - broilers and on which female quails from daily age raised on cellular batteries in communities on 50 heads in conditions adequate to the requirement. Fed in plenty with the dry forages prepared according to 'Recommendations on the normalized feeding an agricultural bird' (1996), recipes 5-2; 6-1. To chickens in plenty allowed water from auto drinking bowls. Conditions of a micro climate corresponded to hygienic requirements. At carrying out of experiences studied the following parameters: preservation of a livestock, the reason of a withdrawal, weight of a body - weekly up to the end of experience, consumption of a forage, an expense of a forage for 1 kg of a gain have established weight of a body the methods standard in poultry farming. After application of a productive solution took blood from under wings of a vein and in blood have established maintenance of hemoglobin, quantity of red cells, leukocytes and a leukocyte of the formula, the maintenance of calcium and phosphorus. An application of a productive solution for experimental birds for the fifth day has caused clinical symptoms of a poisoning and destruction in groups. At the survived birds in blood it has been established changes biochemical and hematology. Week age chickens of skilled group lagged behind in growth development in comparison with control group on 30 % and at the end of the skilled period skilled have lagged behind in growth and development on 62 %. Among a livestock of chickens of skilled group of a case was more on 53 % on comparisons with the

  20. Analysis of some biochemical and haematological parameters for Mucuna pruriens (DC) seed powder in male rats.

    Science.gov (United States)

    Chukwudi, Ndukwe Henry; Simeon, Omale; Chinyere, Aguiyi John

    2011-10-01

    The biochemical and haematological effects of the seed powder of Mucuna pruriens in male rats were evaluated to establish some biological properties of this potential biopesticide currently undergoing investigation. The result showed that Mucuna pruriens seed extract produced a significant (p<0.05) increase in white blood cell (WBC) count, as well as in bilirubin concentrations, alkaline phosphatase (ALP), protein and creatinine levels measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly reduced (P<0.05) in comparison with the experimental control. PCV, Hb, albumin level and WBC differential counts gave no significant difference between treated and control groups. The results revealed metabolic imbalance in the rats which suggests a mild cholestasis effect of the extract.

  1. Effect of irradiated corn on some biochemical parameters or growing albino rats

    International Nuclear Information System (INIS)

    El-Shennawy, H.M.

    2015-01-01

    This study was conducted to examine the effects of gamma irradiation treatment on the chemical composition of raw and irradiated corn gluten (CG) at 8 and 10 kGy, alongside the impacts irradiated CG consumptions on weight reduction and certain blood biochemical factors in growing rats. Male Sprague-Dawley rats (n=60) were fed the experimental diet for 5 weeks. They were then randomly divided into four groups and fed the iso caloric experimental diets. Food intake, daily body weight gain, apparent food conversion efficiency, plasma and hepatic lipid variables were assessed.The results revealed that the chemical composition of irradiated CG showed non-significant differences relative to the raw one.

  2. Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaie, Somaieh; Nikzad, Hossein; Mahabadi, Javad Amini; Taghizadeh, Mohsen; Azami-Tameh, Abolfazl; Taherian, Aliakbar; Sajjadian, Seyyed Mohammad Sajjad; Kamani, Mehran

    2016-09-01

    Reproductive toxicity is one of the side effects of cyclophosphamide (CP) in cancer treatment. Pumpkin seeds and Zingiber officinale are natural sources of antioxidants. We investigated the possible protective effect of combined pumpkin seed and Zingiber officinale extracts on sperm characteristics, epididymal histology and biochemical parameters of CP-treated rats. Male adult Wistar rats were divided randomly into six groups. Group 1, as a control, received an isotonic saline solution injection intraperitoneally (IP). Group 2 were injected IP with a single dose of CP (100 mg/kg) once. Groups 3 and 4 received CP plus 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract (50:50). Groups 5 and 6 received only 300 and 600 mg/kg combined pumpkin seed and Zingiber officinale extract. Six weeks after treatment, sperm characteristics, histopathological changes and biochemical parameters were assessed. In CP-treated rats, motile spermatozoa were decreased, and abnormal or dead spermatozoa increased significantly (P < 0.001) but administration of the mixed extract improved sperm parameters. Epididymal epithelium and fibromascular thickness were also improved in extract-treated rats compared to control or CP groups. Biochemical analysis showed that the administration of combined extracts could increase the total antioxidant capacity (TAC) level significantly in groups 3, 4, 5 and 6. Interestingly, the mixed extract could decrease most of the side effects of CP such as vacuolization and separation of epididymal tissue. Our findings indicated that the combined extracts might be used as a protective agent against CP-induced reproductive toxicity.

  3. Effect of dietary supplementation of organic zinc on laying performance, egg quality and some biochemical parameters of laying hens.

    Science.gov (United States)

    Abd El-Hack, M E; Alagawany, M; Amer, S A; Arif, M; Wahdan, K M M; El-Kholy, M S

    2018-04-01

    This study was conducted to evaluate the effects of zinc methionine (Zn-Met) supplementation on the performance, egg quality, antioxidant status and some biochemical parameters of blood serum in laying hens from 22 to 34 weeks of age. A total of 120 Hisex Brown laying hens of 22-week-old were randomly allocated into five treatments with six cage replicates for each (four hens/replicate). Dietary treatments consisted of the basal diet with no Zn-Met supplementation (control group) and basal diet supplemented with 25, 50, 75 or 100 mg Zn-Met/kg diet. No significant differences were observed on body weight, body weight gain or feed conversion ratio due to dietary Zn-Met supplementation. However, highly significant impact was observed on daily feed intake. Egg number, egg weight and egg mass were increased in the group fed diet supplemented with the highest level of Zn-Met (100 mg/kg of diet) as compared to other groups. All egg quality traits were statistically (p > .05 or .01) affected as a response to dietary Zn-Met supplementation except egg shape index, shell percentage and yolk index. In comparison with the control group, dietary supplementation of 25, 50, 75 or 100 mg Zn-Met/kg decreased serum triglyceride and LDL-cholesterol levels. Serum cholesterol level was increased with all dietary levels of Zn-Met in comparison with the control group. Dietary Zn-Met supplementation increased the serum content of zinc, where the highest values were recorded with 50 and 100 mg Zn-Met/kg diet. Dietary Zn-Met levels did not affect the antioxidant indices in blood serum except for the activity of copper-zinc superoxide dismutase (Cu-Zn-SOD). The activity of Cu-Zn-SOD was increased with Zn-Met supplementations with no differences among supplemental zinc levels. It is concluded that dietary Zn-Met supplementation reduced serum triglyceride, LDL-cholesterol and increased Zn status and resulted in promoting antioxidant ability of laying hens, and the addition of 100 mg Zn

  4. Comparison of hemodynamic, biochemical and hematological parameters of healthy pregnant women in the third trimester of pregnancy and the active labor phase

    Directory of Open Access Journals (Sweden)

    Flisser Ana

    2011-05-01

    Full Text Available Abstract Background Pregnancy is accompanied by several hemodynamic, biochemical and hematological changes which revert to normal values after labor. The mean values of these parameters have been reported for developed countries, but not for Mexican women. Furthermore, labor constitutes a stress situation, in which these factors may be altered. It is known that serologic increase of heat shock protein (Hsp 70 is associated with abnormal pregnancies, presenting very low level in normal pregnant women. Nevertheless, there are no studies where these measurements are compared in healthy pregnant women at their third trimester of pregnancy (3TP and the active labor phase (ActLP. Methods Seventy five healthy Mexican pregnant women were included. Hemodynamic, biochemical and hematological parameters were obtained in all cases, and serum Hsp70 levels were measured in a sample of 15 women at 3TP and at ActLP. Results Significant differences were found in most analysis performed and in Hsp70 concentration at 3TP as compared to ActLP, however all were within normal range in both conditions, supporting that only in pathological pregnancies Hsp70 is drastically increased. Conclusion Results obtained indicate that 3TP and ActLP have clinical similarities in normal pregnancies, therefore if abnormalities are found during 3TP, precautions should be taken before ActLP.

  5. THE REFERENCE VALUES OF THE MAIN BIOCHEMICAL PARAMETERS OF THE HEMOLYMPH OF APIS MELLIFERA CARPATHICA IN SOUTH-EASTERN ROMANIA

    Directory of Open Access Journals (Sweden)

    AGRIPINA SAPCALIU

    2007-10-01

    Full Text Available Although biochemical analyses of the blood were and are still used for the routinediagnosis and especially for the metabolic survey in farm animals, such analyses, maybe applied for the honeybee hemolymph (as a paraclinic examination. The aim of thisexperimental study was to investigate and to determine the reference values of themain biochemical parameters in the hemolymph of the healthy honeybees of Apismellifera carpathica. The honeybee samples were collected in order to analyze thehealth condition of the respective colony. All the samples coming from sick colonieswere removed, only healthy adult honeybees coming from strong colonies were keptand used for hemolymph collection. By special methods, samples of hemolymph (300μl/sample collected from about 50 individuals, were analyzed both during the activeseason (spring/summer and the inactive season (autumn/winter. The study wascarried out on 50 samples of undiluted hemolymph taken from a total number of about2,500 honeybees. The following 21 biochemical parameters were analyzed: GLU(mg/dl, HDL-c (mg/dl, ALP (UI/l, T-cho (mg/dl, Tprot (mg/dl, Alb. (g/dl, BUN(mg/dl, LDH (UI/l, CPK (UI/l, Mg (mg/dl, FRA (μm/l, IP (mg/dl, GGT (UI/l,GOT (UI/l, GPT (UI/l, Ca (mg/dl, Cre (mg/dl, Amy (UI/l, T–BIL (mg/dl, TG(mg/dl, UA (mg/dl. The test was carried out after the collection and processing of thesamples using the SPOTCHEM EZSP4430, equipment with dry kits, the slides technique,respectively.

  6. Biochemical and neurophysiological parameters in hemodialyzed patients with chronic renal failure

    NARCIS (Netherlands)

    Schoots, A.C.; Vries, de P.M.J.M.; Thiemann, R.C.J.; Hazejager, W.A.; Visser, S.L.; Oe, P.L.

    1989-01-01

    Serum concentrations of accumulated solutes, standard clinical biochemistry, and parameters of clinical neuropathy, were determined in hemodialyzed patients with chronic renal failure. Analyses by high-performance liquid chromatography included creatinine, pseudouridine, urate, p-hydroxyhippuric

  7. Long-term changes in microbial and biochemical parameters in the Central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Raghukumar, C.; Nath, B.N.; Sharma, R.; LokaBharathi, P.A.; Dalal, S.G.

    an artificial sediment disturbance was created by a hydraulic benthic disturber. Besides the above- mentioned parameters, extracellular enzymes, alkaline phosphatase, aminopeptidase and lipase were also monitored at these 9 locations. Postdisturbance (PoD1...

  8. The influences of ambient temperature and crude protein levels on performance and serum biochemical parameters in broilers.

    Science.gov (United States)

    Liu, Q W; Feng, J H; Chao, Z; Chen, Y; Wei, L M; Wang, F; Sun, R P; Zhang, M H

    2016-04-01

    This study was undertaken to investigate the effects of ambient temperature, crude protein levels and their interaction on performance and serum biochemical parameters of broiler chickens. A total of 216 Arbor Acre broiler chickens (108 males and 108 females) were used in a 2 × 3 factorial arrangement and randomly reared at two temperatures (normal temperature: 23 °C; daily cyclic high temperature: 28-32 °C) and fed on three diets with different crude protein levels (153.3, 183.3 or 213.3 g/kg, with constant essential amino acids) from 28 to 42 days of age. Daily cyclic high ambient temperature decreased final body weight, average daily weight gain, average daily feed intake and serum total protein contents (p chickens was interacted by daily cyclic high ambient temperature and dietary crude protein levels (p = 0.003). These results indicated that daily cyclic high ambient temperature had a great effect on performance and serum biochemical parameters in broiler chickens, whereas dietary crude protein levels affected them partially. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  9. IMPACT OF LIQUID NITROGEN EXPOSURE ON SELECTED BIOCHEMICAL AND STRUCTURAL PARAMETERS OF HYDRATED Phaseolus vulgaris L. SEEDS.

    Science.gov (United States)

    Cejas, Inaudis; Rivas, Maribel; Nápoles, Lelurlys; Marrero, Pedro; Yabor, Lourdes; Aragón, Carlos; Pérez, Aurora; Engelmann, Florent; Martínez-Montero, Marcos Edel; Lorenzo, José Carlos

    2015-01-01

    It is well known that cryopreserving seeds with high water content is detrimental to survival, but biochemical and structural parameters of cryostored hydrated common bean seeds have not been published. The objective of this work was to study the effect of liquid nitrogen exposure on selected biochemical and structural parameters of hydrated Phaseolus vulgaris seeds. We cryopreserved seeds at various moisture contents and evaluated: germination; electrolyte leakage; fresh seed weight; levels of chlorophyll pigments, malondialdehyde, other aldehydes, phenolics and proteins; thickness of cotyledon epidermis, parenchyma, and starch storage parenchyma; and radicle and plumule lengths. Germination was totally inhibited when seeds were immersed in water for 50 min (moisture content of 38%, FW basis) before cryopreservation. The combined effects of seed water imbibition and cryostorage decreased phenolics (free, cell wall-linked, total), chlorophyll a and protein content. By contrast, electrolyte leakage and levels of chlorophyll b and other aldehydes increased as a result of the combination of these two experimental factors. These were the most significant effects observed during exposure of humid seed to liquid nitrogen. Further studies are still required to clarify the molecular events taking place in plant cells during cryostorage.

  10. Understanding alterations on blood and biochemical parameters in athletes that use dietary supplements, steroids and illicit drugs.

    Science.gov (United States)

    Bordin, Dayanne Mozaner; Bettim, Bárbara Beltrame; Perdona, Gleici Castro; de Campos, Eduardo Geraldo; De Martinis, Bruno Spinosa

    2017-02-01

    In recent years it was verified there are an alarming growing number of teenagers and young adults using a combination of dietary supplements (DS) anabolic androgenic steroids (AAS) and drugs of abuse. This practice is used to improve physical fitness and appearance, may cause serious side effects. This article shows the alterations in the hematological and renal function parameters associate with these substances in 40 athletes. This research involved three steps: 1-the administration of a self-completion questionnaire ; 2-the assessment of hematological and biochemical parameters of renal function and; 3-toxicological urinalysis. Hematological and biochemical tests were conducted in an accredited laboratory and the toxicological urinalysis was validated in our laboratory using liquid-liquid extraction (LLE) and gas chromatography-mass spectrometry (GC-MS). The testosterone levels in the participants who consumed steroids increased 20-60% and alterations in serum creatinine, urea and uric reached values of up to 1.9; 60.6 and 7.5mg/dL, respectively. The toxicological urinalysis supports self-reports confirming the use of AAS and recreational drugs, putting at risk the health of those athletes increasing the chances of kidney diseases. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  11. Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters

    DEFF Research Database (Denmark)

    Ferk, Franziska; Mišík, Miroslav; Nersesyan, Armen

    2016-01-01

    SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out...... if XN causes alterations of health-related parameters in humans. METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability......'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism...

  12. STUDY OF BIOCHEMICAL PARAMETERS IN AMNIOTIC FLUID FOR ASSESSMENT OF FOETAL MATURITY IN CASES OF NORMAL PREGNANCY

    Directory of Open Access Journals (Sweden)

    Leela

    2015-11-01

    Full Text Available Assessment of foetal maturity had been proven of value in evaluating the foetal condition. Accurate assessment of foetal maturity is essential for the proper timing of delivery in various risk pregnancies. Amniotic Fluid analysis for foetal maturity had been of proven value. In the present study, study of biochemical parameters in amniotic fluid in respect of Creatinine, Uric Acid, Urea, Total Proteins, and Electrolytes i.e. Sodium, Potassium and Chloride has been done, along with Serum Electrolytes. Standard methodologies were adopted. The observations in the present study correlated with the works of Chadick et al and Pitkin and Zwirek. The levels of Creatinine, Uric Acid and Urea in Amniotic Fluid showed elevation, while Total Proteins and Serum Sodium showed a decline, as gestation progressed. The Serum and Amniotic Fluid Potassium and Chloride levels remain almost constant throughout the pregnancy. Thus, it is observed that the use of multiple parameters is desirable for accurate assessment of foetal maturity.

  13. Design Parameter Optimization of a Silicon-Based Grating Waveguide for Performance Improvement in Biochemical Sensor Application.

    Science.gov (United States)

    Hong, Yoo-Seung; Cho, Chun-Hyung; Sung, Hyuk-Kee

    2018-03-05

    We performed numerical analysis and design parameter optimization of a silicon-based grating waveguide refractive index (RI) sensor. The performance of the grating waveguide RI sensor was determined by the full-width at half-maximum (FWHM) and the shift in the resonance wavelength in the transmission spectrum. The transmission extinction, a major figure-of-merit of an RI sensor that reflects both FWHM and resonance shift performance, could be significantly improved by the proper determination of three major grating waveguide parameters: duty ratio, grating period, and etching depth. We analyzed the transmission characteristics of the grating waveguide under various design parameter conditions using a finite-difference time domain method. We achieved a transmission extinction improvement of >26 dB under a given bioenvironmental target change by the proper choice of the design procedure and parameters. This design procedure and choice of appropriate parameters would enable the widespread application of silicon-based grating waveguide in high-performance RI biochemical sensor.

  14. A survey on the biochemical parameters in serum of the Azarbaijan buffaloes with TRP

    Directory of Open Access Journals (Sweden)

    Gh. Mousavi

    2010-02-01

    Full Text Available TRP disease is one of the prevalent disease in cows and buffaloes that most common in dairy cows feed by prepared foods but cases occur infrequently in buffaloes, beef cattle, sheep and goats. In this research 15 Azerbaijan buffaloes with TRP were studied. Firstly affected buffaloes were diagnosed based on clinical signs. The blood samples were obtained from the related animals and after separation blood serum by centrifuging, the biochemical tests, such as sodium, potassium, Glucose, phosphorus, albumin and calcium levels in serum were measured. The mean levels of sodium, potassium, Glucose, phosphorus, albumin and calcium in buffaloes with TRP were 112 mEq/Lit, 3.11 mEq/Lit, 42.29 mg/dl, 3.84 mg/dl, 544 mg/dl, 8.60 mg/dl. In this study the mean levels of sodium, potassium, phosphorus in buffaloes with TRP was less than the normal buffaloes and the mean levels of calcium, Glucose as such as the normal animals and the mean levels of albumin was higher than the normal buffaloes.

  15. Serial assessment of biochemical parameters of red cell preparations to evaluate safety for neonatal transfusions.

    Science.gov (United States)

    Mukherjee, Somnath; Marwaha, Neelam; Prasad, Rajendra; Sharma, Ratti Ram; Thakral, Beenu

    2010-12-01

    Neonatologists often prefer fresh blood (2,3 diphosphoglycerate (2,3 DPG) up to 21 days of storage. Within each group of RBC, rise in mean concentration of potassium, lactate and plasma haemoglobin from day 1 to 21 of storage was significant in CPDA-1 RBC having the highest levels at day 21. From day 3 to 21, SAGM RBC had higher mean pH value than CPDA-1 RBC though this difference was not statistically significant. SAGM RBC had highest mean glucose concentration during storage than other two types of red cell preparations (P2,3 DPG concentration from day 1 to 7 was significant (P<0.05). A positive correlation existed between mean plasma potassium and haemoglobin in all three types of red cells (r=0.726, 0.419, 0.605 for CPDA-1 RBC, SAGM RBC and whole blood respectively, P<0.005). All the three red cell preparations tested revealed biochemical changes within acceptable limits of safety till 21 days of storage. CPDA-1 RBCs had the highest degree of these changes.

  16. Anthropometric and biochemical parameters in adolescents and their relationship with eating habits and household food availability.

    Science.gov (United States)

    Cardoso Chaves, Otaviana; Franceschini, Sylvia do Carmo Castro; Machado Rocha Ribeiro, Sônia; Ferreira Rocha Sant Ana, Luciana; Garçon de Faria, Carlos; Priore, Silvia Eloiza

    2013-01-01

    To evaluate the per capita availability of energy and macronutrients in the home and frequency of food consumption by adolescents and to relate them with anthropometric and biochemical variables, as well as verify if the eating habits of parents are associated to the children. We evaluated the weight, height, body fat (%BF), glucose, insulin, triglycerides, total cholesterol (TC) and fractions of 120 adolescents. We evaluated also the eating habits of adolescents and their parents. Adolescents with more than 35% of available lipids had 9.1-fold higher chances of presenting alterations in TC. Those who replaced the main meals for snacks had 3.66, 4.66 and 2.82 higher chances of presenting alterationsin %BF, insulin and triglycerides, respectively. The daily consumption of fruit was considered as a protective factor in relation to hyperinsulinemia. There was a similar feeding behavior among adolescents and their mothers. The results suggest the importance of specific attention to adolescent health, focused on family education. Copyright © AULA MEDICA EDICIONES 2013. Published by AULA MEDICA. All rights reserved.

  17. Serial assessment of biochemical parameters of red cell preparations to evaluate safety for neonatal transfusions

    Science.gov (United States)

    Mukherjee, Somnath; Marwaha, Neelam; Prasad, Rajendra; Sharma, Ratti Ram; Thakral, Beenu

    2010-01-01

    Background & Objectives: Neonatologists often prefer fresh blood (diphosphoglycerate (2,3 DPG) up to 21 days of storage. Results: Within each group of RBC, rise in mean concentration of potassium, lactate and plasma haemoglobin from day 1 to 21 of storage was significant in CPDA-1 RBC having the highest levels at day 21. From day 3 to 21, SAGM RBC had higher mean pH value than CPDA-1 RBC though this difference was not statistically significant. SAGM RBC had highest mean glucose concentration during storage than other two types of red cell preparations (P<0.005). Within each group, fall in mean 2,3 DPG concentration from day 1 to 7 was significant (P<0.05). A positive correlation existed between mean plasma potassium and haemoglobin in all three types of red cells (r=0.726, 0.419, 0.605 for CPDA-1 RBC, SAGM RBC and whole blood respectively, P<0.005). Interpretation & Conclusions: All the three red cell preparations tested revealed biochemical changes within acceptable limits of safety till 21 days of storage. CPDA-1 RBCs had the highest degree of these changes. PMID:21245620

  18. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Comparison of malnutrition inflammation score, anthropometry and biochemical parameters in assessing the difference in protein-energy wasting between normal weight and obese patients undergoing haemodialysis.

    Science.gov (United States)

    Alipoor, Elham; Hosseinzadeh-Attar, Mohammad Javad; Mahdavi-Mazdeh, Mitra; Yaseri, Mehdi; Zahed, Narges S

    2017-07-01

    Protein-energy wasting (PEW) is prevalent in haemodialysis. Obesity is an independent risk factor of kidney insufficiency, but it is proposed to have beneficial roles in better outcomes in the final stage of disease. Better nutritional status and body reserves are among probable mechanisms, but direct examinations are limited. The present study aimed to investigate whether obese patients have preferable nutritional status compared to normal weight patients based on malnutrition inflammation score (MIS) and other PEW parameters in haemodialysis. This case-control study investigated 52 normal weight (18.5 < body mass index (BMI) < 25 kg/m 2 ) and 48 obese (BMI≥30 kg/m 2 ) patients on regular haemodialysis. PEW was assessed based on anthropometric and biochemical factors, recent weight changes, appetite, anorexia, dietary intake and MIS. Obese patients had better MIS compared with the normal weight group (P < 0.001), although varying degrees of wasting were prevalent among this group too (75% mild and 25% moderate wasting). The obese group had less significant weight loss (4.2 vs 8%) and anorexia and better appetite. However, a considerable percentage of patients in both groups showed muscle (94.6% of normal weight and 19.5% of obese) and peripheral fat tissue (89.2% of normal weight and 31.7% of obese) losses compared to the 50th percentile. Biochemical parameters were not significantly different between groups except for triglyceride (P = 0.001), transferrin and total iron-binding capacity (P = 0.028). MIS was significantly better in obese patients; however, both groups showed degrees of wasting based on MIS and other PEW parameters. Nutritional status of obese haemodialysis patients should be monitored regularly because of high risk of PEW like other BMI categories. © 2017 Dietitians Association of Australia.

  20. Outcomes and biochemical parameters following cardiac surgery: effects of transfusion of residual blood using centrifugation and multiple-pass hemoconcentration.

    Science.gov (United States)

    McNair, Erick; McKay, William; Qureshi, Abdul Mohamed; Rosin, Mark; Gamble, Jon; Dalshaug, Greg; Mycyk, Taras; Prasad, Kailash

    2013-12-01

    To determine whether or not there was a significant difference between the methods of centrifugation (CF) and multiple-pass hemoconcentration (MPH) of the residual cardiopulmonary-bypass volume in relation to biochemical measurements and patient outcomes. Prospective, randomized, and controlled. Conducted at a western Canadian tertiary care hospital. Consisted of 61 consecutive male and female patients from ages 40 to 80 who were scheduled for cardiac surgery with cardiopulmonary bypass. Either the centrifugation or multiple-pass hemoconcentration method was used to process the residual blood from the cardiopulmonary bypass circuit. The 12-hour postoperative levels of serum hemoglobin were not significantly different in the centrifugation group as compared to the multiple-pass hemoconcentration group. However, the serum levels of total protein and albumin were significantly higher in the multiple-pass hemoconcentration group as compared to the centrifugation group. Additionally, after 12-hours postoperatively, the serum fibrinogen and platelet counts were significantly higher in the multiple-pass hemoconcentration group as compared to those of the centrifugation group. The allogeneic product transfusion index and the chest-tube blood drainage indices were lower in the multiple-pass hemoconcentration group as compared to the centrifugation group. Although the CF method provided a product in a shorter turnaround time, with consistent clearance of heparin, the MPH method trended towards enhanced biochemical and clinical patient outcomes over the 12-hour postoperative period. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Effects of Discontinuous Administration of Dietary Mannan oligosaccharide and β-1,3-glucan on Hematological and Blood Serum Biochemical Parameters of Rainbow trout (Oncorhynchus mykiss

    Directory of Open Access Journals (Sweden)

    Reza Akrami

    2016-04-01

    Full Text Available Introduction Bans and restrictions of antibiotics as feed additives in fish culture in many countries have resulted in the increase in studies on alternative dietary supplements such as prebiotics to enhance the health and production of cultured fish. Prebiotics are nondigestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon. In addition, prebiotics promote the growth of lactic acid bacteria that are beneficial to health and lessen the density of pathogenic microorganisms. Because of the conservation of immune system, it is suggested that alternate administration of different immunostimulants may activate different parts of immune system of shrimps and take advantage of different immunostimulants to solve the problem of immunity fatigue and enhance the immunity continuously. However, there is no report on the discontinuous administration of immunostimulants for rainbow trout. Thus, considering the strategic importance of this species, the goal of this study was to assess the effects of discontinuous administration of dietary mannan oligosaccharide and β-1,3-glucan on hematological and blood serum biochemical parameters of rainbow trout. Materials and Methods A 6-week trial was conducted to compare the effects of discontinuous administration of dietary mannan oligosaccharide and β-1,3-glucan on hematological and blood serum biochemical parameters of rainbow trout (Oncorhynchus mykiss. Four feeding strategies were set, including feeding prebiotic-free diet continuously (control, feeding dietary prebiotic continuously (1.5 g kg-1, feeding dietary alternately (one week prebiotic+one week control diet and 2 Day prebiotic+5 Day control diet. Juveniles were fed the experimental diet in rate of 4% of the body weight per day, spread across two feeding time. At the end of the experiment, three fish were sampled randomly from each tank and were

  2. Influence of dietary slow-release urea on growth performance, organ development and serum biochemical parameters of mutton sheep.

    Science.gov (United States)

    Ji, S K; Zhang, F; Sun, Y K; Deng, K D; Wang, B; Tu, Y; Zhang, N F; Jiang, C G; Wang, S Q; Diao, Q Y

    2017-10-01

    Eighty Dorper × thin-tailed Han cross-bred non-castrated male lambs [mean body weight (BW), 25.87 ± 1.06 kg] were randomly allocated to one of five different concentrations of slow-release urea (urea phosphate, UP). The feed consisted of an equal amount of concentrate diet and roughage; the concentrate feed was formulated to be isoenergetic and isonitrogenic and contained 0%, 1%, 2%, 4% and 8% UP (UP0.0, UP1.0, UP2.0, UP4.0 and UP8.0, respectively) as a replacement for soya bean meal. Feed intake, BW, average daily gain (ADG), feed utilisation efficiency (FUE), absolute and relative organ weights and biochemical and histopathological parameters were measured. Feed intake, BW, ADG and FUE significantly decreased in the group receiving UP8.0 (p  0.05). Quadratic equations were developed between the UP dosage in the concentrate feed and ADG or FUE (r 2  = 0.973 for ADG and r 2  = 0.761 for FUE) to determine the appropriate dosage of UP given the desire to maximise either ADG or FUE, the appropriate dosage (feed concentration) was calculated as 2.01% UP to achieve the greatest ADG or 2.13% UP to achieve the best FUE. The relative weight of the liver (% BW) in the UP2.0 groups was significantly greater than that of UP0.0 (p  0.05). The UP8.0 treatment significantly increased serum phosphorus levels (p < 0.05) and decreased the levels of alkaline phosphatase, glucose and calcium (Ca) compared with the lower UP dosage (p < 0.05). No histopathological differences were found in either hepatic tissues or renal tissues among treatments. Dietary UP as a replacement for soya bean in concentrate feeds for mutton sheep should not exceed 4%, as higher dosing may cause malnutrition and mineral disorders. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  3. Immunological and biochemical parameters of patients with metabolic syndrome and the participation of oxidative and nitroactive stress

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    A.N.C. Simão

    2011-07-01

    Full Text Available Metabolic syndrome (MS is a multifactorial disease involving inflammatory activity and endothelial dysfunction. The aim of the present study was to evaluate the relationship between the changes in lipoperoxidation, in immunological and biochemical parameters and nitric oxide metabolite (NOx levels in MS patients. Fifty patients with MS (4 males/46 females and 50 controls (3 males/47 females were studied. Compared to control (Mann-Whitney test, MS patients presented higher serum levels (P < 0.05 of fibrinogen: 314 (185-489 vs 262 (188-314 mg/dL, C-reactive protein (CRP: 7.80 (1.10-46.50 vs 0.70 (0.16-5.20 mg/dL, interleukin-6: 3.96 (3.04-28.18 vs 3.33 (2.55-9.63 pg/mL, uric acid: 5.45 (3.15-9.65 vs 3.81 (2.70-5.90 mg/dL, and hydroperoxides: 20,689 (19,076-67,182 vs 18,636 (15,926-19,731 cpm. In contrast, they presented lower (P < 0.05 adiponectin: 7.11 (3.19-18.22 vs 12.31 (9.11-27.27 µg/mL, and NOx levels: 5.69 (2.36-8.18 vs 6.72 (5.14-12.43 µM. NOx was inversely associated (Spearman’s rank correlation with body mass index (r = -0.2858, P = 0.0191, insulin resistance determined by the homeostasis model assessment (r = -0.2530, P = 0.0315, CRP (r = -0.2843, P = 0.0171 and fibrinogen (r = -0.2464, P = 0.0413, and positively correlated with hydroperoxides (r = 0.2506, P = 0.0408. In conclusion, NOx levels are associated with obesity, insulin resistance, oxidative stress, and inflammatory markers. The high uric acid levels together with reactive oxygen species generation may be responsible for the reduced NO levels, which in turn lead to endothelial dysfunction. The elevated plasma chemiluminescence reflecting both increased plasma oxidation and reduced antioxidant capacity may play a role in the MS mechanism.

  4. Trajectories of CKD-MBD biochemical parameters over a 2-year period following diagnosis of secondary hyperparathyroidism: a pharmacoepidemiological study.

    Science.gov (United States)

    Filipozzi, Pierre; Ayav, Carole; Ngueyon Sime, Willy; Laurain, Emmanuelle; Kessler, Michèle; Brunaud, Laurent; Frimat, Luc

    2017-03-27

    To define groups of patients according to the changes of biochemical parameters, that is, serum calcium, phosphate and parathyroid hormone (PTH), over a 2-year follow-up period using group-based multi-trajectory modeling (GBMM) among a cohort of dialysis patients with newly diagnosed secondary hyperparathyroidism (SHPT) (ie, PTH≥500 ng/L for the first time) and to compare their patient characteristics and treatments. Pharmacoepidemiological study. In the 12 dialysis units located in the French region of Lorraine. A total of 269 dialysis patients with newly diagnosed SHPT were prospectively included from December 2009 to May 2012 and followed-up for 2 years. We identified four distinct trajectory groups: 'rapid PTH drop' experiencing a rapid and sharp decrease (over weeks) in PTH level associated with decreasing phosphate level within normal range (n=34; 12.7%), 'gradual PTH decrease' experiencing a gradual and continuous decrease (over months) in PTH level and maintaining phosphate at a middle level throughout the study (n=98; 36.4%), 'slow PTH decrease with high phosphate' experiencing a slow decrease in PTH level associated with a relatively high phosphate level (n=105; 39.0%) and 'uncontrolled SHPT' with high levels of PTH and phosphate throughout the study (n=32; 11.9%). Patients in the 'uncontrolled SHPT' group were significantly (p<0.00001) younger than patients in other groups. Kidney Disease Improving Global Outcomes (KDIGO) targets for PTH, phosphate and calcium were reached simultaneously for 14.9% of patients at baseline and 16.7% at the end of the study. Patients were given cinacalcet more frequently at months 3 and 6 in the 'rapid PTH drop' and at month 24 in the 'uncontrolled SHPT' groups. Over 2 years following a new SHPT diagnosis, a younger age and a higher rate of alkaline phosphatase were associated to a continuous uncontrolled SHPT. Patients with the lowest PTH at the end of the follow-up tended to receive more often cinacalcet. Clinical

  5. Distribution of haptoglobin types and their significance for changes in biochemical and immunological parameters of people irradiated at small doses

    International Nuclear Information System (INIS)

    Tel'nov, V.I.; Vologodskaya, I.A.; Zhuntova, G.V.

    1995-01-01

    Haptoglobin (Hp) polymorphism and its significance for changes in biochemical and immunological parameters was analyzed in personnel of a nuclear plant receiving doses of external and internal irradiation close to maximum permissible levels. The distribution of Hp types and frequencies of alleles Hp in the group of probands as a whole (1-1, 14.1; 2-1, 49.2%; and 2-2, 36.7%) and in probands subjected to different types and doses of irradiation (1-1, 13.6 - 16.0%; 2-1, 47.8 - 50.5%; and 2-2, 35.8 - 38.2%) did not differ from those expected or from corresponding parameters of the control group and population. An increase in the frequency of allele Hp 1 was observed for persons over 60 years of age. It was found that the levels of uric acid, the activities of acid phosphatase, lactate dehydrogenase, alkaline phosphatase and its isozymes, and the content of certain lymphocyte classes and immune complexes are initially higher in carriers of Hp type 1-1. In these probands, changes in certain parameters related to irradiation were observed less frequently. In some cases, opposite changes in immunological parameters were observed in persons with different Hp types after internal irradiation. 23 refs., 6 tabs

  6. Effect of Different Growth Conditions on Certain Biochemical Parameters of Different Cyanobacterial Strains

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    Hammouda, O. E.

    2012-01-01

    Full Text Available Aims: Variation in the traditional growth medium conditions to enhance the production of lipids, carbohydrates, protein and the free amino acids content of three cyanobacterial species. Methodology and Results: Three species of cyanobacteria (Anabaena laxa, Anabaena fertilissima and Nostoc muscorum were collected from the culture collection of Soils, Water and Environment Research Institute, Agriculture Research Center, Giza, Egypt, to investigate their biochemical composition under different growth conditions, using BG110 (nitrogen free as growth medium. These conditions were represented by control medium, static glucose medium with (1%, w/v, aerated medium (aerated by bubbling technique depending on CO2 normally existed in air with a concentration of 0.03%, molasses medium (0.7%, v/v and aerated medium enriched with glucose (1%, w/v. Lipid content, total carbohydrates, soluble proteins and free amino acids were determined at the previous conditions. Glucose at 0.7% (w/v was the most favorable for lipid production in A. laxa, where it exhibited the highest lipid content (427 μg/g fresh wt.. Increasing molasses concentration up to 0.7% (v/v produced an increase in lipid contents of the tested cyanobacterial strains. The highest lipid content of both N. muscorum (366.2 μg/g fresh wt. and A. laxa (357.4 μg/g fresh wt. were recorded at molasses concentrations of 0.1 and 0.7% (v/v, respectively. A. laxa expressed high significant values for both proteins (31.6 μg/mL and free amino acids (40.5 mg/g dry wt. after 6 days of incubation period under aerated enriched glucose condition (1%, w/v. Also, at the same growth conditions, A. fertilissima exhibited high significant values for carbohydrates at 4th day (876.8 mg/g dry wt.. Conclusion, significance and impact of study: Aerated enriched glucose medium (1%, w/v was the best growth medium condition used in the present study.

  7. Comparative study of native and irradiated crotoxin. Biochemical and pharmacological effects

    International Nuclear Information System (INIS)

    Nascimento, N. do.

    1991-01-01

    Ionizing radiation is able to detoxify several venoms, including snake venom, without affecting significantly their antigenic and immunogenic properties. In order to elucidate this phenomena, we conceived a comparative biochemical and pharmacological study between native and gamma irradiated (2000Gy) crotoxin, main toxin of south american rattlesnake Crotalus durissus terrificus. Crotoxin was isolated and purified from crude venom by molecular exclusion chromatography, pI precipitation and then irradiated. Immunodiffusion, electrophoresis and gel filtration showed that the molecular integrity was preserved after irradiation with some higher molecular weight aggregate formation and maintenance of its antigenic capacity. The antibodies induced by irradiated toxin had a similar titer to the antibodies induced by native crotoxin; however with higher protective effects in mice. Crotoxin toxicity became 15 times lower after irradiation, as determined by LD sub(50) in mice. Native and irradiated crotoxin biodistribution occurred with a similar general pattern, with renal elimination. In contrast to irradiated crotoxin, the native crotoxin is initially retained in kidneys. A later concentration (2-3hs) occurs in phagocyticmononuclear cells rich organs (liver and spleen) and neural junctions rich organs (muscle and brain). (author)

  8. Effect of Dietary Cysteine Supplementation on Growing Performance, Pelt Quality and Some Serum Biochemical Parameters of Young Rex Rabbit

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    Tossou Myrlene Carine B

    2013-01-01

    Full Text Available The present study was conducted to evaluate the effects of dietary cysteine, a sulphur containing amino acid supplementation on growth performance, pelt quality and a number of serum biochemical parameters of young Rex Rabbit. One hundred and twenty Rex Rabbits aged 45 days were divided into five dietary treatment groups including one control group and 4 experimental groups. Each group was composed of 24 animals and was fed with different diets for 56 days corresponding to the fattening period. Diet was incorporated with 0% of cysteine (control group and 0,10%, 0,20%, 0,30% and 0,40% respectively for the experimental groups. At the end of the experiment, results showed that cysteine supplementation to the diet affected average daily gain, live weight,skin length, skin area and pelt weight at the late phase of 42 to 56 days (P0.05

  9. Analysis Of Clinical, Haematological And Biochemical Parameters In Patients With Infectious Mononucleosis

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    Canović Petar

    2015-12-01

    Full Text Available Primary infection with Epstein-Barr virus (EBV usually occurs in early childhood and often does not present clinical symptoms. More than 90% of adults are infected with this virus. A primary infection that occurs in adolescence or adulthood is usually clinically presented as infectious mononucleosis with a triad of symptoms: fever, lymphadenopathy and pharyngitis. Our retrospective study included 51 patients with a median age of 17 (9-23 years and serologically confirmed infectious mononucleosis. All patients with infectious mononucleosis were treated at the Clinic for Infectious Diseases at the Clinical Center in Kragujevac during 2013. We analysed the clinical, haematological and laboratory parameters of patients. The aspartate-aminotransferase levels were increased in 40 patients, with a mean value of 116.24 (±93.22; the alanine-aminotransferase levels were increased in 44 patients, with a mean value of 189.24 (±196.69. Lymphadenopathy was the most common clinical feature upon admission in 49 patients (96%; 38 patients (74.5% had splenomegaly, and 20 (39% had hepatomegaly. Twenty-six patients (51% had leukocytosis with lymphocytosis, while 15 (75% of the 20 who had a normal leukocyte count also had lymphocytosis. In the present study, we updated the clinical, haematological and laboratory parameters, which may lead to the establishment of an accurate diagnosis and promote further treatment of the patients.

  10. Thyroid function and some related biochemical parameters in bilharzial liver disease

    International Nuclear Information System (INIS)

    Ibrahim, W. Abdel Massih

    1983-01-01

    Bilharziasis in its different forms is estimated to affect at least 200 million people in the world (W.H.O., 1973). In egypt, it affects 60 - 70% of the rural population (WcMullen, 1963). Bilharzial ova reaches the liver with the portal blood stream causing a fibro - cellular reaction in and around the portal tract resulting in hepatic periportal fibrosis with various clinical stages (Abdalla et al., 1978 b). The effect of liver disease on thyroid function is still a subject of controversy and contradiction. While some reports are published on the effect of non-bilharzial liver disease on thyroid function, there is scanty studies as regards to bilharzial liver diseases . Most of the studies carried out to evaluate thyroid function in bilharzial liver disease, were focused on the estimation of one or two parameters of thyroid function e.g., T 3 and TSH. The available review of literature was focused on some parameters of thyroid function ignoring the others thyroid function tests and not considering the hormone carrier proteins. Thus resulting inconsistent and contradictory results (Ghareeb, 1962; El Haieg and Ibrahim, 1977)

  11. Biochemical adaptations in middle-distance runners: an assessment of blood and anthropometric parameters

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    Danila Di Majo

    2014-12-01

    Full Text Available In order to understand the mechanism underlying the physiological adaptation of purely aerobic workout, we investigated the effect of 2 months of training on nine males (17-22 year-old middle distance running agonistic athletes. Blood sample was collected in the morning to analyze: hematological parameters, lipid profile, liver function enzymes [glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, gamma-glutamyl transferase (γ-GT] and skeletal and myocardial markers of muscle damage [creatin kinase (CK and creatin kinase MB (CK-MB]. Endurance training, as it implies high oxygen consumption, should increase reactive oxygen species, but it has been shown that exercise leads to increased activation of antioxidant defenses. In fact, serum levels of γ-GT enzyme and total CK were not increased. On the other hand, a statistical significant reduction of CKMB has been observed. There were not variations in hematological parameters. As far as the anthropometric value is concerned, after two months of training there was a change in weight (P<0.0001. Finally, any oxidative and biological stress was highlighted in the middle distance runners but, since this is a preliminary study, it would be of interest to replicate the study on a larger sample.

  12. Evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries

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    A Kaur

    2012-01-01

    Full Text Available Aim: The aim of the present study was the evaluation of non-microbial salivary caries activity parameters and salivary biochemical indicators in predicting dental caries. Materials and Methods: The present study was carried out on 60 children, aged 4-6 years, selected from the schools of Panchkula district, Haryana, on the basis of their caries status. Level of hydration, flow rate, pH, buffering capacity, relative viscosity, calcium, phosphorus and alkaline phosphatase levels in caries-free and caries-active children were evaluated. Results: Results showed that 90% of subjects in the caries-free group and 30% of subjects in the caries-active group had normal level of hydration value of less than 60 s and the difference was found to be statistically very highly significant. Normal flow rate of stimulated saliva was found in 90% of the subjects in caries-free group and 33.3% subjects in the caries active group and difference was found to be statistically very highly significant. Adequate salivary pH was found in 100% subjects in caries-free group and 30% in caries-active group and the difference was statistically very highly significant. Conclusion: To conclude, within limitations of this study, it became clear that normal level of hydration and higher values for flow rate, pH, buffering capacity of saliva lead to good oral health and a reduced caries occurrence. Increased salivary viscosity plays a role in increasing caries incidence. Salivary biochemical indicators like calcium, phosphorus and alkaline phosphatase also play their respective role in determining caries susceptibility of an individual. These salivary parameters can be used as diagnostic tool for caries risk assessment.

  13. INTERRELATION AMONG SERUM LITHIUM LEVELS AND BONE METABOLISM AND SOME BIOCHEMICAL PARAMETERS IN PRE AND POST-MENAUPOSAL WOMEN

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    Ruken Esra Demirdöğen

    2016-08-01

    Full Text Available The target of this study is to determine the interrelation among serum Li level on bone metabolism (Ca, P, Parathormon, and Vitamin-D, sex and metabolic hormones (estrogen, FSH, LH and TSH, and some biochemical parameters in premenopausal and postmenopausal women. The study is carried out with 10 women: 5 premenopausal and 5 postmenopausal women. The serum Li levels, bone metabolism indicators (i.e., ALP, Ca, P, Mg, Cu, Zn and some biochemical parameters such as serum tryglyceride, alkalene phosphatase, total cholesterol, HDL, LDL and cholesterol levels were determined. The estrogen blood level of women in menopause period was found to be lower than that of women in pre-menopause period (p<0.01 and the FSH level was found to be higher (p<0.01. In the lipid profile the triglyceride level in the post-menopause period was found to be low (p<0.05 and HDL (p<0.001, LDL (p<0.001 and the cholesterol levels were found to be high (p<0.001. The alkalene phosphatase (p<0.001 and Vitamin-D levels (p<0.001 were found to decrease. When the mineral levels were investigated no meaningful difference was observed in the serum magnesium and copper levels while zinc (p<0.01 and phosphorus (p<0.005 levels were observed to increase, the calcium levels (p<0.05 decreased and Li levels considerably decreased (p<0.0001. According to the results obtained it was determined for the first time that Li defficiency can be related with menopause and the related diseases and thus Li therapy can be used in developing new treatment protocols of menopause as an alternative method.

  14. Effects of Dietary Electrolyte Balance on Growth Performance, Nitrogen Metabolism and Some Blood Biochemical Parameters of Growing Rabbits

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    J. W. Li

    2013-12-01

    Full Text Available The effects of different dietary electrolyte balance (DEB on growth performance, nitrogen (N metabolism and some blood biochemical parameters were investigated in 2 to 3 months old growing rabbits. A total of 150 growing rabbits of 2 months age were randomly divided into five groups according to average body weight, with 30 rabbits in each group. The DEB levels of the five experimental diets were −154, −3.16, +201, +347, and +500 meq/kg of dry matter (DM, respectively. There was a 7-d adaptation period and a 23-d experimental period. The results showed that the DEB levels had a quadratic affect on the average daily feed intake (ADFI (p<0.001. The greatest ADFI was achieved when the DEB level was +201 meq/kg DM. Fecal N (FN content linearly decreased (0.047, while digestible N (DN, retained N (RN, efficiency of intake N converted into digestible N (DN/IN and the efficiency of intake N converted into retained N (RN/IN linearly increased with the DEB increase (0.020, 0.004, 0.021, and 0.049, respectively. Serum phosphorus (P ion content linearly increased with the DEB increase (p = 0.036. The DEB had a quadratic relationship with serum anion gap (AG (p = 0.002 and serum parathyroid hormone (PTH content (p = 0.016. The DEB levels quadratically affected base excess (BE in the plasma (p<0.001. In conclusion, the DEB unaffected growth performance but affected feed intake, N metabolism and some blood biochemical parameters of growing rabbits.

  15. Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing system.

    Science.gov (United States)

    Bubel, F; Dobrzański, Z; Gaweł, A; Pogoda-Sewerniak, K; Grela, E R

    2015-01-01

    An influence of various humic-plant feed additives based on some herbs (nettle, chamomile, yarrow, perforatum), lucerne and humic materials on biochemical indices of Lohmann Brown (LB) layers blood plasma was estimated. Hens were housed in deep litter system, 20 birds in a group. Four groups were formed: control (C - standard feeding), and experimental, supplemented with prepara- tions: E-1 herbal-humic, E-2 humic-herbal and E-3--humic-lucerne. Hens were placed in the pens on the 16th week of life, addition of preparations with standard food mixture started at the 22nd wk and lasted until 66th wk of life. Blood for analyses was collected four times in the following periods: 27, 37, 54 and 65th wk of life. The applied humic-plant preparations to a limited degree affected the values of examined biochemical parameters in serum: total protein (TP), albumins (Albs), glucose (Glu), urea, triacylglycerols (TAG), total cholesterol (TCh), alanine aminotransferase (ALT), aspartate aminot- ransferase (AST) and alkaline phosphatase (ALP). It is difficult to determine based on these study, which preparation is one the most active biologically, however is seems that humic-lucerne prepara- tion affected the examined blood parameters to the highest degree. The reference values ranges in hens blood serum LB hens were proposed for: TP (43-65 g/l), Albs (15-22 g/l), urea (0.5-1.2 mmol/l), Glu 10-15 mmol/l), TCh (2.2-4.5 mmol/l), TAG (10-24 mmol/l), AST (4-12 U/l), ALT (150-280 mmol/l) and ALP (190-350 U/l).

  16. Cytomorphologic spectrum of lymphocytic thyroiditis and correlation between cytological grading and biochemical parameters

    Science.gov (United States)

    Anila, KR; Nayak, Nileena; Jayasree, K

    2016-01-01

    Introduction: Chronic lymphocytic thyroiditis [Hashimoto thyroiditis (HT)] is a common thyroid lesion diagnosed on fine-needle aspiration cytology (FNAC). Apart from FNAC, various other parameters, such as clinical features, ultrasonographic findings, antithyroid antibody levels, hormone profiles, and radionuclide thyroid scan, are also taken into consideration in making a diagnosis of HT. Aims: To grade lymphocytic thyroiditis based on the cytomorphology and to correlate the cytological grades with the levels of antithyroid peroxidase antibody (ATPO), antithyroglobulin antibody (ATG), and thyroid stimulating hormone (TSH). Materials and Methods: During a period of one and half years, 1,667 cases underwent FNAC of thyroid at our tertiary care center. Of these, 128 cases had cytological evidence of lymphocytic thyroiditis. Out of these, in 60 cases the levels of ATPO, ATG, and TSH were known. The cytological grades of lymphocytic thyroiditis in these cases were correlated with these parameters. Results: Out of the 60 cases, 55 were females. Age ranged from 5 years to 70 years, with majority of patients in third decade. Diffuse enlargement of thyroid was the commonest presentation. However, 14 cases presented with nodular disease. Majority of the patients had grade 1 thyroiditis (27 cases), followed by grade 2 thyroiditis (22 cases). Cytomorphology was diagnostic of thyroiditis in all 60 cases. ATPO was elevated in 57 cases and ATG was elevated in 40 cases. Elevated level of TSH was seen in only 18 cases. In 39 cases, TSH value was normal. There was no correlation between the cytological grades of thyroiditis and the levels of antithyroid antibodies and TSH. Conclusion: Lymphocytic infiltration of thyroid follicles is pathognomonic of lymphocytic thyroiditis. Positivity for antithyroid antibodies is strongly associated with HT but no correlation was observed between the grades of thyroiditis and the levels of ATPO, ATG, and TSH. PMID:27756987

  17. A New Data Analysis System to Quantify Associations between Biochemical Parameters of Chronic Kidney Disease-Mineral Bone Disease.

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    Mariano Rodriguez

    Full Text Available In hemodialysis patients, deviations from KDIGO recommended values of individual parameters, phosphate, calcium or parathyroid hormone (PTH, are associated with increased mortality. However, it is widely accepted that these parameters are not regulated independently of each other and that therapy aimed to correct one parameter often modifies the others. The aim of the present study is to quantify the degree of association between parameters of chronic kidney disease and mineral bone disease (CKD-MBD.Data was extracted from a cohort of 1758 adult HD patients between January 2000 and June 2013 obtaining a total of 46.141 records (10 year follow-up. We used an advanced data analysis system called Random Forest (RF which is based on self-learning procedure with similar axioms to those utilized for the development of artificial intelligence. This new approach is particularly useful when the variables analyzed are closely dependent to each other.The analysis revealed a strong association between PTH and phosphate that was superior to that of PTH and Calcium. The classical linear regression analysis between PTH and phosphate shows a correlation coefficient is 0.27, p<0.001, the possibility to predict PTH changes from phosphate modification is marginal. Alternatively, RF assumes that changes in phosphate will cause modifications in other associated variables (calcium and others that may also affect PTH values. Using RF the correlation coefficient between changes in serum PTH and phosphate is 0.77, p<0.001; thus, the power of prediction is markedly increased. The effect of therapy on biochemical variables was also analyzed using this RF.Our results suggest that the analysis of the complex interactions between mineral metabolism parameters in CKD-MBD may demand a more advanced data analysis system such as RF.

  18. Evaluation of sublethal toxicity of zinc and chromium in Eudrilus eugeniae using biochemical and reproductive parameters.

    Science.gov (United States)

    Basha, P Mahaboob; Latha, V

    2016-05-01

    Zinc (Zn) and chromium (Cr) act as essential nutrients; however, they can be toxic at higher concentrations. In this study, earthworm species Eudrilus eugeniae was studied for its sensitivity to sublethal doses of Cr (8 ppm) and Zn (350 ppm) in terms of alterations occurred in oxidative stress indices and reproductive parameters in the testis. A significant (p food intake was observed on 7, 14 and 21 days of Cr toxicity; while increased rate was evident upon 7 days of Zn exposure. Changes evident in the rate of cocoon production (-39.54 and +38.63 %), hatchability (-77.85 and +30.0 %) highlight higher toxic potential of Cr than Zn, respectively. Moderate to severe vacuolization in spermatic follicles and higher incidence of tailless (+52.6 % in Cr and 20.8 % in Zn), and head bent (+18.8 % in Cr and 0 % in Zn) sperms were evident in Cr(VI) exposed worms emphasizing the higher vulnerability of E. eugeniae to Cr toxicity. A significant (p implied from results that excessive free radical production and inadequate antioxidant defenses have lead to morphological alterations in sperms which sequentially reduced the reproductive rate.

  19. Basic biochemical, hematological and hormonal parameters for monitoring the health and nutritional status in athletes

    Directory of Open Access Journals (Sweden)

    Aritz Urdampilleta

    2014-09-01

    Full Text Available Sporting competitions are becoming more demanding in terms of intensity of effort, and this means controlling all aspects that affect athletic performance. Food, hydration and supplementation, before, during and after training or competition directly affect health, body composition, performance and recovery of the athlete. The assessment of nutritional status is required for proper advising of the athlete, through blood tests to control the process of adaptation to training. The aim of this paper is to provide practical tools for dietitiansnutritionists to control the health and nutritional status of athletes, as well as monitoring their adaptation to workloads and competition periods. Performing analytical tests to control of protein metabolism, lipid profile, ions, blood tests and iron metabolism, in addition to review some hormonal parameters, may be of interest in order to observe the potential existence of overtraining states. The correct understanding and interpretation of laboratory tests (under sports doctor’s supervision will be most important and useful for dietitiansnutritionists, performing dietary and nutritional advice to athletes, because it will determine the status of the athlete and propose different individual feeding strategies depending on the training phase and response.

  20. The effect of ramadan fasting on biochemical and performance parameters in collegiate wrestlers.

    Science.gov (United States)

    Mirzaei, Bahman; Rahmani-Nia, Farhad; Moghadam, Mahdi Ghahremani; Ziyaolhagh, Seyed Javad; Rezaei, Abolfazl

    2012-11-01

    This study was designed to evaluate the effect of Ramadan fasting on body composition, aerobic and anaerobic power, strength, plasma lipids profile and serum glucose among collegiate wrestlers. Fourteen male collegiate wrestlers (age, 20.12±2.5 yrs) volunteered as subjects for the study. Subjects were tested one week before the beginning of Ramadan, the last two days of Ramadan and the last two days of the 4th week after the end of Ramadan. The paired sample t-test was used to assess the differences in pre and post-performance tests and repeated measures ANOVA and Bonferroni post hoc test to determine differences between three blood samplings. The results showed that except for anaerobic power and strength, body weight, body fat percentage and aerobic power at 4th week of Ramadan were significantly lower than pre-Ramadan values (P=0.05). Total cholesterol and low density lipoprotein levels decreased after Ramadan compared to pre-Ramadan (P= 0.011, P=0.001), however, a month after Ramadan, it reached to higher levels compared to pre-Ramadan period, which were not statistically significant. Similarly, significant decrease and increase were consequently observed in glucose and high density lipoprotein (P=0.001, P=0.045). Triacylglycerol and VLDL increased at the end of Ramadan compared to the period of time before Ramadan, and after Ramadan, it reached the lower levels compared to concentrations before Ramadan (P=0.133, P=0.133). This study also indicated that Ramadan fasting appears to have significant effect on body composition, aerobic power and lipid profile.

  1. INFLUENCE OF SURFACE-ACTIVE RHAMNOLIPID BIOCOMPLEX AND ETHYLTHIOSULFANILATE ON GROWTH AND BIOCHEMICAL PARAMETERS OF PLANTS IN THE OIL CONTAMINATED SOIL

    Directory of Open Access Journals (Sweden)

    A. R. Banya

    2015-10-01

    Full Text Available The aim of the work was to study the influence of rhamnolipid biocomplex and ethylthiosulfanilate on field pea and sorghum plants when growing in petroleum contaminated soil. Plant seeds were treated with solutions of rhamnolipid biocomplex or ethylthiosulfanilate (0.01 g/l before planting and grown in containers with soil artificially contaminated with petroleum (5, 8 and 10%. Effect of rhamnolipid biocomplex and ethylthiosulfanilate was determined by the determination of growth (weight, length plants and biochemical parameters (content of photosynthetic pigments, hydrogen peroxide, malondialdehyde. The stimulating effect of rhamnolipid biocomplex and ethylthiosulfanilate on growth parameters of field pea was shown: the shoot mass has significantly increased on 39%, root mass – on 26% if compared with the control. For sorghum somewhat smaller increase of growth parameters was observed. Under the influence of rhamnolipid biocomplex and ethylthiosulfanilate the content of photosynthetic pigments in field pea and sorghum has also increased. It was determined that the action rhamnolipid biocomplex and ethylthiosulfanilate promoted the decrease of indicators of plant oxidative reactions if compared with control: the content of hydrogen peroxide – in average on 15% and 16%, malondialdehyde – on 13.5% and 16% respectively. The results of the study testify to the effectiveness of rhamnolipid biocomplex and ethylthiosulfanilate as growth stimulators for field pea and sorghum, as well as improvement of adaptive capability of plants to unfavorable conditions. It creates the prospects of their application as effective and ecologically safe substances for the intensification of contaminated soil phytoremediation.

  2. Effect of vitamin D3 on behavioural and biochemical parameters in diabetes type 1-induced rats.

    Science.gov (United States)

    Calgaroto, Nicéia Spanholi; Thomé, Gustavo Roberto; da Costa, Pauline; Baldissareli, Jucimara; Hussein, Fátima Abdala; Schmatz, Roberta; Rubin, Maribel A; Signor, Cristiane; Ribeiro, Daniela Aymone; Carvalho, Fabiano Barbosa; de Oliveira, Lizielle Souza; Pereira, Luciane Belmonte; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina

    2014-08-01

    Diabetes is associated with long-term complications in the brain and reduced cognitive ability. Vitamin D3 (VD3 ) appears to be involved in the amelioration of hyperglycaemia in streptozotocin (STZ)-induced diabetic rats. Our aim was to analyse the potential of VD3 in avoiding brain damage through evaluation of acetylcholinesterase (AChE), Na(+) K(+) -adenosine triphosphatase (ATPase) and delta aminolevulinate dehydratase (δ-ALA-D) activities and thiobarbituric acid reactive substance (TBARS) levels from cerebral cortex, as well as memory in STZ-induced diabetic rats. Animals were divided into eight groups (n = 5): control/saline, control/metformin (Metf), control/VD3 , control/Metf + VD3 , diabetic/saline, diabetic/Metf, diabetic/VD3 and diabetic/Metf + VD3 . Thirty days after treatment, animals were submitted to contextual fear-conditioning and open-field behavioural tests, after which they were sacrificed and the cerebral cortex was dissected. Our results demonstrate a significant memory deficit, an increase in AChE activity and TBARS levels and a decrease in δ-ALA-D and Na(+) K(+) -ATPase activities in diabetic rats when compared with the controls. Treatment of diabetic rats with Metf and VD3 prevented the increase in AChE activity when compared with the diabetic/saline group. In treated diabetic rats, the decrease in Na(+) K(+) -ATPase was reverted when compared with non-treated rats, but the increase in δ-ALA-D activity was not. VD3 prevented diabetes-induced TBARS level and improved memory. Our results show that VD3 can avoid cognitive deficit through prevention of changes in important enzymes such as Na(+) K(+) -ATPase and AChE in cerebral cortex in type 1 diabetic rats. Copyright © 2014 John Wiley & Sons, Ltd.

  3. RELATIONSHIP BETWEEN SOME BIOCHEMICAL PARAMETERS IN HEALTHY AND DAIRY COWS WITH SUBCLINICAL KETOSIS

    Directory of Open Access Journals (Sweden)

    Gülşah Akgül

    2017-07-01

    Full Text Available The aim of the presented study is to compare beta-hydroxy butyric acid (BHBA, glucose (GLU gamma-glutamyl transferase (GGT, phosphorus (P, calcium (Ca, and albumin (Alb values of healthy animals and animals suffering from subclinical ketosis. The material in this study consisted of 15 healthy cows and 15 cows with subclinical ketosis on a commercial dairy farm located in Bursa (Turkey. Significant differences (p<0.001 were observed in BHBA, GLU, and Ca between the two groups. Results of this study might indicate the importance of calcium metabolism in the pathogenesis of the disease.

  4. effect of drinking tea on some chemical and biochemical parameters in biological fluids

    International Nuclear Information System (INIS)

    El-sayed, N.A.S.

    2002-01-01

    Egyptians are known to consume large quantities of black tea . The present study revealed that, urea, creatinine, ALT,AST,ALP, total T 4 , serum levels of Mn, Al and Cr, were significantly (p 7 tea cups/day) compared to control group ( 1-2 tea cups /day). however, significant change was observed in total T 3 and T 4 /T 3 ratio between the two groups. body weight was decreased significantly (p 3 , t 4 and Cr and positively with creatinine serum level of Mn was significantly (P<0.05) negative correlated with serum level of Al in heavy drinking tea group

  5. [Evaluation of various biochemical parameters in the urine and blood of painters in the carpentry business].

    Science.gov (United States)

    Sołtys, M; Jakubowska, W; Lenart, E; Nagadowska, H; Lisiecka, M; Ziemer, I; Koćmierkska-Grodzka, D

    1986-01-01

    The work has been aimed at investigating the effects of organic solvent-toluene-upon the painters of the Building Joinery Factory. Studies carried out during 1978-1980 showed an increased level of toluene metabolite (hippuric acid) in painters' urine, as compared to the environmental control group. The highest level of hippuric acid in painters' urine was found in the first period (first six months) of their job. This was not related to an increase in toluene vapours at the workplace. In painters' blood some irregular and rather small modifications of enzymatic activity were exhibited.

  6. BIOCHEMICAL PARAMETERS FOR Piaractus mesopotamicus, Colossoma macropomum (Characidae AND HYBRID TAMBACU (P. mesopotamicus X C. macropomum

    Directory of Open Access Journals (Sweden)

    Marcos Tavares-Dias

    2010-06-01

    Full Text Available A study for investigating the values of plasma glucose,serum total protein and serum ions (calcium, potassium, magnesium,sodium and chloride was carried out in young Colossomomamacropomum Cuvier, 1818, Piaractus mesopotamicus Holmberg,1887 and the hybrid tambacu (P. mesopotamicus x C. macropomum,kept in intensive system. Glucose concentrations were higher intambacu than in P. mesopotamicus and C. macropomum. Totalprotein levels were higher in P. mesopotamicus than both C.macropomum and tambacu. C. macropomum presented higherconcentrations of sodium and chloride, while P. mesopotamicushad higher concentrations of potassium and magnesium.However, levels of calcium were similar for the species studied.The hybrid tambacu showed the smallest levels of total protein,and intermediate levels of sodium, potassium, magnesium andchloride, compared to both P. mesopotamicus and C. macropomum.Baseline values for healthy fish in aquaculture were established,and they may be used for further comparisons in studies with wildpopulations of P. mesopotamicus and C. macropomum.

  7. Effects of chlorogenic acid, caffeine, and coffee on behavioral and biochemical parameters of diabetic rats.

    Science.gov (United States)

    Stefanello, Naiara; Schmatz, Roberta; Pereira, Luciane Belmonte; Rubin, Maribel A; da Rocha, João Batista Teixeira; Facco, Graziela; Pereira, Maria Ester; Mazzanti, Cinthia Melazzo de Andrade; Passamonti, Sabina; Rodrigues, Marília Valvassori; Carvalho, Fabiano Barbosa; da Rosa, Michelle Melgarejo; Gutierres, Jessie Martins; Cardoso, Andréia Machado; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina

    2014-03-01

    Diabetes mellitus (DM) is associated with brain alterations that may contribute to cognitive dysfunctions. Chlorogenic acid (CGA) and caffeine (CA), abundant in coffee (CF), are natural compounds that have showed important actions in the brain. The present study aimed to evaluate the effect of CGA, CA, and CF on acetylcholinesterase (AChE), Na(+), K(+)-ATPase, aminolevulinate dehydratase (δ-ALA-D) activities and TBARS levels from cerebral cortex, as well as memory and anxiety in streptozotocin-induced diabetic rats. Animals were divided into eight groups (n = 5-10): control; control/CGA 5 mg/kg; control/CA 15 mg/kg; control/CF 0.5 g/kg; diabetic; diabetic/CGA 5 mg/kg; diabetic/CA 15 mg/kg; and diabetic/CF 0.5 g/kg. Our results demonstrated an increase in AChE activity and TBARS levels in cerebral cortex, while δ-ALA-D and Na(+), K(+)-ATPase activities were decreased in the diabetic rats when compared to control water group. Furthermore, a memory deficit and an increase in anxiety in diabetic rats were observed. The treatment with CGA and CA prevented the increase in AChE activity in diabetic rats when compared to the diabetic water group. CGA, CA, and CF intake partially prevented cerebral δ-ALA-D and Na(+), K(+)-ATPase activity decrease due to diabetes. Moreover, CGA prevented diabetes-induced TBARS production, improved memory, and decreased anxiety. In conclusion, among the compounds studied CGA proved to be a compound which acts better in the prevention of brain disorders promoted by DM.

  8. Unlimited access to low-energy diet causes acute malnutrition in dams and alters biometric and biochemical parameters in offspring.

    Science.gov (United States)

    do Nascimento, E; de Santana Muniz, G; das Graças de Santana Muniz, M; de Souza Alexandre, L; da Rocha, L S; Leandro, C G; de Castro, R M; Bolaños-Jimenez, F

    2014-02-01

    Here we analyze the outcomes of unlimited access to a low-energy (LE) diet in dams and their offspring. At 3 weeks' gestation, pregnant Wistar rats were divided into two groups: (1) the control group received a normoenergetic diet; and (2) the experimental group received the LE diet. In dams, lactation outcomes, food intake, body weight, plasma IGF-1, prealbumin, transferrin and retinol-binding protein levels were evaluated; in offspring, biometric and biochemical parameters and food intake were evaluated. No differences were observed during pregnancy. However, after lactation, dams that received the LE diet demonstrated significant reductions in body weight (P<0.05), plasma IGF-1 (P=0.01), prealbumin and visceral fat (P<0.001). Pups born to dams that received the LE diet demonstrated reduced body length and weight at weaning (P<0.001) and were lighter than the control animals at the end of the experimental period. Pups also demonstrated reduced plasma, low-density lipoprotein (P=0.04), triglycerides (P=0.002) and glucose levels (P<0.05), and differences were noted in visceral fat. These results indicate that feeding dams with LE diet during the reproductive period induces acute malnutrition and impairs the growth and development of offspring, as well as certain metabolic parameters.

  9. Effect of spirulina food supplement on blood morphological parameters, biochemical composition and on the immune function of sportsmen

    Directory of Open Access Journals (Sweden)

    K Milasius

    2009-07-01

    Full Text Available Of highest biological value are natural concentrates of optimally combined substances produced by nature. One of food supplements of this kind is dietary Spirulina produced by the Tianshi firm (China. It is a most rationally balanced food supplement of a high biological value; it satisfies the needs of the whole body, including its immune system. The aim of the current work was to assess the effect of the multicomponent natural food supplement Spirulina on the physical development, blood morphological, biochemical picture and immune function of sportsmen. Materials and Methods: The study cohort comprised 12 high performance sportsmen (age 20-22 years. They were using tablets of Spirulina, a dietary product for 14 days. Physical development was determined with the aid of standard methods. The general blood picture was analyzed with the aid of a Micros-60 hematological analyzer (company ABX DIAGNOSTICS, France. Lymphocytes and their subsets were analysed by flow cytometery (FACSCalibur, Becton Dickinson Immunocytometry Systems (BDIS, USA and the absolute and percentage values were calculated. To evaluate immune function lymphocyte blasttransformation response to mitogens was studied. Results: Investigations carried out on endurance-training sportsmen showed that a 14-d administration of Spirulina exerted a positive effect on blood morphological composition indices and its biochemical changes. The results of our study confirm the positive effect of Spirulina food supplement on the quantitative parameters of immune system. Part of the study cohort after weeks showed a tendency of normalizing CD3 , CD3 CD4 lympocite count: positive changes were still present two weeks following the interruption of Spirulina intake

  10. DIETARY AND BIOCHEMICAL PARAMETERS OF THE NIACIN DEFICIENCY IM YOUNG RATS

    Directory of Open Access Journals (Sweden)

    CARLOS ROBERTO VICTORIA

    2009-07-01

    Full Text Available

    ABSTRACT: Using a defined laboratory diet composed by gelatin (6% and casein (10% as protein sources, without tryptophan and nicotinic acid supplementation, it should be possible to reproduce, in young rats, after 28 dais feeding with this deficient diet, a clinical and laboratory model of niacin deficiency. The deficient group, when compared against control, showed poor growth (63.3 g x 163.3 g; p<0.05, lower urinary excretion of N’-methylnicotinamide (0.005 mg/24 h x 0.259 mg/24 h; p<0.05, lower plasma free-tryptophan (2.1 uM/dl x 5.4 uM/dl; p<0.05, lower plasma albumin level (1.8 g/dl; p<0.05, smaller erythrocytes (48.9 u3 x 54.8 u3; p<0.05 and lower corpuscular hemoglobin (23.2 pg x 26.6 pg; p<0.05. All these changes were normalized by niacin replacement. We conclude that this dietetics model is easy and practical to be used for niacin deficiency purposes. KEYWORDS: Niacin; nicotinicacid; tryptophan; N’-methylnicotinamide; Pellagra.

  11. The Association between some Incidental Factors and Biochemical Parameters Linked to Atherogenesis

    International Nuclear Information System (INIS)

    Mohamed, M.I.; Kamal, A.M.; Aldahaby, M.A.

    2008-01-01

    The present study was conducted to assess the role played by some factors including age, residence location, diet, hypertension and job pattern, as incidental factors in the atherogenic process, on the lipid, lipoprotein, homocysteine, vitamin B 12 and folic acid in apparently healthy Egyptian males. Sera from one hundred and forty six apparently healthy male volunteers were analyzed for total cholesterol, triglycerides, high density Iipoproteins cholesterol (HDL-c), low density lipoproteins cholesterol (LDL-c), apolipoprotein A. (Apo At), apolipoprotein B ( Apo B ) and homocysteine, in addition to vitamin B I2 and folic acid. Data from the present study showed that middle aged individuals (over 40 years), urban volunteers, butter users, volunteers experiencing mental work and hypertensives experienced increased lipid fractions when compared to their counterparts. The data also showed the lack of an effect of the previous factors on serum homocysteine, folic acid or vitamin B 12 . In view of the previous findings, it may be concluded that factors such as: age, urban residence, butter consumption, hypertension and intellectual work may contribute to the etiology of atherosclerosis through their impact on serum lipid fractions. Serum homocysteine and related vitamins B 12 and folic acid seem to be unmodified by these factors

  12. A comparative method for processing immunological parameters: developing an "Immunogram".

    Science.gov (United States)

    Ortolani, Riccardo; Bellavite, Paolo; Paiola, Fiorenza; Martini, Morena; Marchesini, Martina; Veneri, Dino; Franchini, Massimo; Chirumbolo, Salvatore; Tridente, Giuseppe; Vella, Antonio

    2010-04-01

    The immune system is a network of numerous cells that communicate both directly and indirectly with each other. The system is very sensitive to antigenic stimuli, which are memorised, and is closely connected with the endocrine and nervous systems. Therefore, in order to study the immune system correctly, it must be considered in all its complexity by analysing its components with multiparametric tools that take its dynamic characteristic into account. We analysed lymphocyte subpopulations by using monoclonal antibodies with six different fluorochromes; the monoclonal panel employed included CD45, CD3, CD4, CD8, CD16, CD56, CD57, CD19, CD23, CD27, CD5, and HLA-DR. This panel has enabled us to measure many lymphocyte subsets in different states and with different functions: helper, suppressor, activated, effector, naïve, memory, and regulatory. A database was created to collect the values of immunological parameters of approximately 8,000 subjects who have undergone testing since 2000. When the distributions of the values for these parameters were compared with the medians of reference values published in the literature, we found that most of the values from the subjects included in the database were close to the medians in the literature. To process the data we used a comparative method that calculates the percentile rank of the values of a subject by comparing them with the values for others subjects of the same age. From this data processing we obtained a set of percentile ranks that represent the positions of the various parameters with regard to the data for other age-matched subjects included in the database. These positions, relative to both the absolute values and percentages, are plotted in a graph. We have called the final plot, which can be likened to that subject's immunological fingerprint, an "Immunogram". In order to perform the necessary calculations automatically, we developed dedicated software (Immunogramma) which provides at least two different

  13. White popular (Populus alba L.) - Litter impact on chemical and biochemical parameters related to nitrogen cycle in contaminated soils

    Energy Technology Data Exchange (ETDEWEB)

    Ciadamidaro, L.; Madejon, P.; Cabrera, F.; Madejon, E.

    2014-06-01

    Aim of study: The aim of this study was to determine the effect of litter from Populus alba on chemical and biochemical properties related to the N cycle in soils with different pH values and trace element contents. We hypothesized that this litter would influence several parameters related to the N cycle and consequently to soil health. Area of study: we collected two reforested contaminated soils of different pH values (AZ pH 7.23 and DO pH 2.66) and a non-contaminated soil (RHU pH 7.19). Materials and methods: Soil samples were placed in 2,000 cm{sup 3} microcosms and were incubated for 40 weeks in controlled conditions. Each soil was mixed with its corresponding litter, and soils without litter were also tested for comparison. Ammonium (NH{sub 4}{sup 4}+-N) and nitrate (NO{sub 3}{sup -} -N) content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN), protease activity, and several chemical properties such as pH, available trace element concentrations (extracted with 0.01 M CaCl{sub 2}) were determined at different times of incubation. Main results: Values of available trace elements did not vary during the incubation and were always higher in acid soil. In neutral soils litter presence increased values of Kjeldahl-N, NO{sub 3} –-N content, potential nitrification rate (PNR), microbial biomass nitrogen (MBN) and protease activity. Presence of trace elements in neutral soils did not alter the parameters studied. However, acidic pH and high content of available trace elements strongly affected NH{sub 4}{sup +}-N and NO{sub 3}{sup -} -N, microbial biomass N and protease activity. Research highlights: Our results showed the negative effect of the acidity and trace element availability in parameters related with the N-cycle. (Author)

  14. White poplar (Populus alba L. - Litter impact on chemical and biochemical parameters related to nitrogen cycle in contaminated soils

    Directory of Open Access Journals (Sweden)

    Paula Madejon

    2014-04-01

    Full Text Available Aim of study: The aim of this study was to determine the effect of litter from Populus alba on chemical and biochemical properties related to the N cycle in soils with different pH values and trace element contents. We hypothesized that this litter would influence several parameters related to the N cycle and consequently to soil health.Area of study: we collected two reforested contaminated soils of different pH values (AZ pH 7.23 and DO pH = 2.66 and a non-contaminated soil (RHU pH 7.19.Materials and methods: Soil samples were placed in 2,000 cm3 microcosms and were incubated for 40 weeks in controlled conditions. Each soil was mixed with its corresponding litter, and soils without litter were also tested for comparison. Ammonium (NH4+-N and nitrate (NO3–-N content, potential nitrification rate (PNR, microbial biomass nitrogen (MBN, protease activity, and several chemical properties such as pH, available trace element concentrations (extracted with 0.01 M CaCl2 were determined at different times of incubation.Main results: Values of available trace elements did not vary during the incubation and were always higher in acid soil. In neutral soils litter presence increased values of Kjeldahl-N, NO3–-N content, potential nitrification rate (PNR, microbial biomass nitrogen (MBN and protease activity. Presence of trace elements in neutral soils did not alter the parameters studied. However, acidic pH and high content of available trace elements strongly affected NH4+-N andNO3–-N, microbial biomass N and protease activity.Research highlights: Our results showed the negative effect of the acidity and trace element availability in parameters related with the N-cycle.Key words: microbial biomass N; protease activity; soil pH; N mineralization; nitrification; phytoremediation.

  15. Evaluation of biochemical and redox parameters in rats fed with corn grown in soil amended with urban sewage sludge.

    Science.gov (United States)

    Grotto, Denise; Carneiro, Maria Fernanda Hornos; Sauer, Elisa; Garcia, Solange Cristina; de Melo, Wanderley José; Barbosa, Fernando

    2013-09-01

    The increased production of urban sewage sludge requires alternative methods for final disposal. A very promising choice is the use of sewage sludge as a fertilizer in agriculture, since it is rich in organic matter, macro and micronutrients. However, urban sewage sludge may contain toxic substances that may cause deleterious effects on the biota, water and soil, and consequently on humans. There is a lack of studies evaluating how safe the consumption of food cultivated in soils containing urban sewage sludge is. Thus, the aim of this paper was to evaluate biochemical and redox parameters in rats fed with corn produced in a soil treated with urban sewage sludge for a long term. For these experiments, maize plants were grown in soil amended with sewage sludge (rates of 5, 10 and 20 t/ha) or not (control). Four different diets were prepared with the corn grains produced in the field experiment, and rats were fed with these diets for 1, 2, 4, 8 and 12 weeks. Biochemical parameters (glucose, total cholesterol and fractions, triglycerides, aspartate aminotransferase and alanine aminotransferase) as well the redox state biomarkers such as reduced glutathione (GSH), malondialdehyde (MDA), catalase, glutathione peroxidase and butyrylcholinesterase (BuChE) were assessed. Our results show no differences in the biomarkers over 1 or 2 weeks. However, at 4 weeks BuChE activity was inhibited in rats fed with corn grown in soil amended with sewage sludge (5, 10 and 20 t/ha), while MDA levels increased. Furthermore, prolonged exposure to corn cultivated in the highest amount per hectare of sewage sludge (8 and 12 weeks) was associated with an increase in MDA levels and a decrease in GSH levels, respectively. Our findings add new evidence of the risks of consuming food grown with urban sewage sludge. However, considering that the amount and type of toxic substances present in urban sewage sludge varies considerably among different sampling areas, further studies are needed to

  16. Study of Okra Powder (Abelmoscus Esculentus Effects on Histology of Liver Tissue and Sero-Biochemical Parameters in Diabetic Rats (HFD/STZ

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    N erfani majd

    2016-12-01

    Full Text Available Introduction: Type 2 diabete is a kind of metabolic disease that it is associated with hyperglycemia, hyperlipidemia and disturbed liver function.  The aim of the present study was to evaluate the protective effects of Okra Powder on liver damage in high fat diet fed / streptozotocin (HFD-STZ-induced type 2 diabetic rats. Methods: In this experimental study, 25 Wistar Albino female rats with an average weight of (200–220 g were randomly divided  into 5 groups: Group I: (control group rats were fed the standard diet, Group II: healthy rats that received Okra Powder (200 mg/kg for 4 weeks; Group III (HFD/STZ group: Rats were fed with high-fat diet (HFD (60% fat for 4 weeks  and then injected low dose of STZ (35 mg/kg, Group IV:  Diabetic rats that received Okra Powder (200 mg/kg for 4 weeks. GroupV: Diabetic rats that received metformin (200 mg/kg for 4 weeks. At the end of experiment, biochemical parameters were measured. Liver samples were removed and 5-6 µ sections were made and stained by H&E and Sudan black staining. Results: The results showed that all the biochemical parameters, except HDL-C and serum insulin were increased in diabetic rats, while they were decreased in Okra supplementation group compared  to diabetic rats (p<0.05. The liver structure alterations were improved in treated diabetic rats with Okra Powder and metformin.  Conclusion: Our findings confirmed the potential anti-hyperglycemic and hypolipidemic effects of Okra Powder. Thus, it seems it has an important role in the management of type 2 diabete.

  17. Biochemical parameters of pregnant rats and their offspring exposed to different doses of inorganic mercury in drinking water.

    Science.gov (United States)

    Oliveira, Cláudia S; Oliveira, Vitor A; Ineu, Rafael P; Moraes-Silva, Lucélia; Pereira, Maria E

    2012-07-01

    This work investigated the effects of low and high doses of inorganic mercury in drinking water on biochemical parameters of pregnant rats and their offspring. Female Wistar rats were treated during pregnancy with 0, 0.2, 0.5, 10 or 50 μg Hg(2+)/mL as HgCl(2). Rats were euthanized on day 20 of pregnancy. Pregnant rats presented a decrease in total water intake in all doses of mercury tested. At high doses, a decrease in the total food intake and in body weight gain was observed. Pregnant rats exposed to 50 μg Hg(2+)/mL presented an increase in kidney relative weight. Mercury exposure did not change serum urea and creatinine levels in any of the doses tested. Moreover, mercury exposure did not change porphobilinogen synthase activity of kidney, liver and placenta from pregnant rats in any of the doses tested, whereas fetuses of pregnant rats exposed to 50 μg Hg(2+)/mL presented an increase in the hepatic porphobilinogen synthase activity. In general, pregnant rats presented alterations due to HgCl(2) exposure in drinking water. However, only the dose 50 μg Hg(2+)/mL appeared to be enough to cross the blood-placenta barrier, since at this dose the fetuses presented change in the porphobilinogen synthase activity. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Correlations between blood lead levels and some physiological and biochemical parameters of nutritional importance in some Nigerian women

    International Nuclear Information System (INIS)

    Ojo, J.O.; Oketayo, O.O.; Salawu, A.A.

    2011-01-01

    Lead is one of the common toxic materials widely occurring in the Nigerian environment. Even with the change to unleaded petrol, other significant sources still remain to be addressed. The deleterious impact of lead on human health, is well documented. The effects range from attention-grabbing mass mortality - as happened in Zamfara State recently, to no less serious but grossly neglected neurocognitive and neurodevelopmental effects, including lowered intelligence quotient scores. All these impacts are most serious in children, especially fetuses who receive their burden from their mothers. Ninety percent of lead in most adults resides in the bones with a half-life measured in decades. Therefore, fetuses and breast-fed children in this generation will remain seriously at risk of exposure to damaging lead levels no matter the efforts to rid our external environment of lead in the years to come. In this work, we have investigated the correlation between Blood Lead Levels (BLL) and up to 35 physiological and biochemical parameters of nutritional importance in 62 women of child-bearing age from lIe-lfe, Nigeria. BLL was determined in venous blood using Inductively- coupled plasma Mass Spectrometry. Our results show that BLL significantly correlates with Packed Cell Volume (PCV), Creatine Clearance, and the ratio of Low-density Lipids to High density Lipids in these women. When the subjects were stratified into different Nutritional Status group based on their Body Mass Index, significant correlation was found between BLL and Age but only in Obese subjects.

  19. Alterations in Somatostatin Cells and Biochemical Parameters Following Zinc Supplementation in Gastrointestinal Tissue of St reptozotocin-Induced Diabetic Rats

    International Nuclear Information System (INIS)

    Bolkent, Sema; Bolkent, Sehnaz; Yanardag, Refiye; Mutlu, Ozgur; Yildirim, Sukriye

    2006-01-01

    Chronic hyperglycemia in diabetes is a major causative factor of free radical generation which further leads to many secondary diabetic complications via the damage to cellular proteins, membrane lipids, and nucleic acids. Zinc is an essential trace element in all living systems and plays a structural role in many proteins and enzymes. Somatostatin is known to have inhibitory effects on various gastrointestinal functions. Therefore, we determined somatostatin protein production and secretion levels, and biochemical and light microscopical changes following zinc supplementation in the gastrointestinal tract of streptozotocin (STZ)-diabetic rats. The animals were divided into four groups: Group I: control (untreated) animals; Group II: control animals given zinc sulfate; Group III: diabetic animals; and Group IV: diabetic animals given zinc sulfate. Zinc sulfate was given to the animals by gavage at a daily dose of 100 mg/kg body weight for 60 days. Diabetes was induced by intraperitoneal (i.p.) injection of STZ in a single dose of 65 mg/kg. For histological studies, stomach and duodenum tissues were fixed in Bouin solution and sections stained with Masson’s trichrome and Periodic-Acid-Schiff. Tissue homogenates were used for protein, lipid peroxidation (LPO), glutathione (GSH), and nonenzymatic glycosylation (NEG) analyses. Zinc supplementation to the STZ-diabetic rats revealed the protective effect of zinc on these parameters. Zinc supplementation may contribute to prevent at least some complications of diabetes mellitus

  20. Changes In Cortisol And Some Biochemical Parameters In Blood Of Egyptian Buffalo Calves Exposed To Two Successive Summer Seasons

    International Nuclear Information System (INIS)

    NESSIM, M.Z.

    2010-01-01

    Ten male buffalo calves were used in the present study. The animals were housed in semi-open barn. Ration was composed of concentrate mixture and wheat straw according to NRC (1985). Animals were allowed to drink fresh water ad libitum. Plasma cortisol, total protein, albumin, globulin, urea, creatinine, GPT and GOT were determined at the end of thermoneutral and summer heat stress exposure periods. Rectal temperature and respiration rate were recorded daily during both periods whereas body weight was recorded weakly during each period. Summer heat stress induced significant increases in rectal temperature, respiration rate, plasma cortisol, albumin/globulin ratio, plasma urea-N, creatinine, GPT and GOT while total protein, albumin, globulin did not affect by summer heat stress. Body weight was decreased under heat stress in both summer seasons but increased with age. In conclusion, summer heat stress induced alterations in some physiological and biochemical parameters of blood and body weight of male buffalo calves, and the young calves were less tolerant to summer heat stress than the older calves.

  1. Quality evaluation of the edible blue-green alga Nostoc flagelliforme using a chlorophyll fluorescence parameter and several biochemical markers.

    Science.gov (United States)

    Gao, Xiang; Yang, Yiwen; Ai, Yufeng; Luo, Hongyi; Qiu, Baosheng

    2014-01-15

    Nostoc flagelliforme is an edible blue-green alga with herbal and dietary values. Due to the diminishing supply of natural N. flagelliforme and the large investment on the development of its cultivation technology, it is anticipated that artificially cultured N. flagelliforme will soon sustain the market supply. Once this change occurs, the storage-associated quality problem will become the focus of attention for future trade. In this paper, we used a chlorophyll fluorescence parameter, maximum quantum efficiency of Photosystem II (Fv/Fm), and several biomarkers to evaluate the quality of several N. flagelliforme samples. It was found that longer storage times resulted in darker coloured solutions (released pigments) and decreased amounts of chlorophyll a (Chl a) and water-soluble sugars (WSS). Additionally, a higher Fv/Fm value suggests better physiological recovery and quality. In actual application, determination of Fv/Fm would be the first step for evaluating the quality of N. flagelliforme, and the biochemical indexes would serve as good secondary markers. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Therapeutic effects of marshmallow (Althaea officinalis L.) extract on plasma biochemical parameters of common carp infected with Aeromonas hydrophila.

    Science.gov (United States)

    Banaee, Mahdi; Soleimany, Vahid; Nematdoost Haghi, Behzad

    2017-01-01

    This study evaluated preclinical and clinical safety of marshmallow ( Althaea officinalis L.) extract as a naturopathic medicine in common carp deliberately infected with Aeromonas hydrophila . The fish were fed 0 (control), 2.50, 5.00 and 10.00 g of marshmallow extract for 60 days in a preclinical experiment and then, challenged with A. hydrophila for a 10-day experiment. Significant increases were observed in aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), creatine phosphokinase (CPK) activities and plasma creatinine levels in fish fed 10 g marshmallow extract per kg feed. However, alanine aminotransferase (ALT) significantly decreased on day 60. The fish fed 2.50 g marshmallow extract per kg feed indicated increased levels of total protein and globulin. There were no significant changes in albumin levels ( p > 0.05). 2.50 and 5.00 g marshmallow significantly decreased triglyceride and cholesterol levels and increased glucose levels ( p Marshmallow extract at 5.00 and 10.00 g can adjust plasma biochemical parameters in fish challenged with A. hydrophila . The results of preclinical studies and pharmaceutical toxicity of marshmallow extract revealed that dietary levels lower than 5.00 g were safe and effective. The results of this clinical study demonstrated that marshmallow extract (5.00 g kg -1 feed) can protect fish against A. hydrophila .

  3. Effects of Operating Parameters on Measurements of Biochemical Oxygen Demand Using a Mediatorless Microbial Fuel Cell Biosensor

    Directory of Open Access Journals (Sweden)

    Min-Chi Hsieh

    2015-12-01

    Full Text Available The conventional Biochemical Oxygen Demand (BOD method takes five days to analyze samples. A microbial fuel cell (MFC may be an alternate tool for rapid BOD determination in water. However, a MFC biosensor for continuous BOD measurements of water samples is still unavailable. In this study, a MFC biosensor inoculated with known mixed cultures was used to determine the BOD concentration. Effects of important parameters on establishing a calibration curve between the BOD concentration and output signal from the MFC were evaluated. The results indicate monosaccharides were good fuel, and methionine, phenylalanine, and ethanol were poor fuels for electricity generation by the MFC. Ions in the influent did not significantly affect the MFC performance. CN− in the influent could alleviate the effect of antagonistic electron acceptors on the MFC performance. The regression equation for BOD concentration and current density of the biosensor was y = 0.0145x + 0.3317. It was adopted to measure accurately and continuously the BOD concentration in actual water samples at an acceptable error margin. These results clearly show the developed MFC biosensor has great potential as an alternative BOD sensing device for online measurements of wastewater BOD.

  4. Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats

    International Nuclear Information System (INIS)

    Abd Elmonem, H.A.; Ali, E.A.

    2011-01-01

    Nutritional Habits have changed significantly and junk foods have become widely popular, in recent years. The present study aimed to shed the light on the effect of potato chips and / or ketchup consumption on some biochemical parameters. Sixty four male and female albino rats were used in the study. Animals were maintained on 0.25 g potato chips/ rat and / or 0.125 g ketchup / rat, 5 days a week for 4 weeks. Potato chips showed the lowest body wt gain in the male rats after 4 weeks but, ketchup modulated this negative effect of the potato chips in the group of male animals fed on potato chips plus ketchup. Potato chips significantly decreased brain serotonin, liver glutathione (GSH) and catalase (CAT) in both sexes; brain dopamine, serum total proteins, albumin, total globulins, α 2 - and β 1 -globulins in the females and serum thyroxine (T 4 ) in the male rats. Ketchup apparently affected serum T 4 and A / G ratio in both sexes, brain dopamine and liver GSH in the males in addition to brain serotonin, serum total globulins and ?1-globulin in the female rats. Potato chips plus ketchup significantly changed T 4 , dopamine, GSH, CAT, α 1 and α 2 -globulins in both sexes; serotonin and β 1 -globulin in the male rats, total proteins and albumin in the females. It could be concluded that potato chips consumption might induce numerous adverse effects in various body organs

  5. Dietary verbascoside supplementation in donkeys: effects on milk fatty acid profile during lactation, and serum biochemical parameters and oxidative markers.

    Science.gov (United States)

    D'Alessandro, A G; Vizzarri, F; Palazzo, M; Martemucci, G

    2017-09-01

    Various uses of donkeys' milk have been recently proposed for human consumption on the basis of its nutritional characteristics. Improvements in milk fatty acid profile and animal oxidative status can be induced through dietary supplementation of phenolic compounds. The study aimed to evaluate in donkeys the effects of dietary supplementation with verbascoside (VB) on: (i) the fatty acid profile and vitamins A and E contents of milk during a whole lactation, and (ii) blood biochemical parameters and markers of oxidative status of the animals. At foaling, 12 lactating jennies were subdivided into two groups (n 6): control, without VB supplement; VB, receiving a lipid-encapsulated VB supplement. Gross composition, fatty acid profile and vitamins A and E contents in milk were assessed monthly over the 6 months of lactation. Serum total cholesterol, high-density lipoproteins cholesterol and low-density lipoproteins cholesterol, tryglicerides, non-esterified fatty acid, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase, reactive oxygen metabolites, thiobarbituric acid reactive substances (TBARs), vitamin A and vitamin E were evaluated at 8 days after foaling (D0) and then at D90, D105 and D120 of lactation. In milk, the VB supplementation decreased the saturated fatty acids (Pdonkey's milk with a benefit on the oxidative status and serum lipidic profile of the animals.

  6. Hatch ability, growth and some blood biochemical parameters in hatching quails following the exposure of incubated eggs to red laser

    International Nuclear Information System (INIS)

    Abu Taleb, A.M.

    2003-01-01

    A total number of 600 Japanese quail eggs were used this study. The eggs were divided into three groups then incubated. Eggs of groups one (G1) and two (G2) were exposed to 30 and 60 minutes, respectively, to the red laser rays (Laser Pointer LD-300) at 0, 7 and 14 days of embryogenesis,while group three (G3) was not treated and served as control. Hatch ability body weight and mortality were recorded weekly for each group. Furthermore, five blood samples were collected from one day old chicks at hatching and at six weeks of age from each group to determine some hematological parameters including RBCs, WBCs, PCV and Hb. Liver enzyme activities (GPT and GOT), kidney function (uric acid and creatinine), cholesterol and total lipids were determined for the 6 weeks old chicks only. The present results indicated that exposure to red to significant increase in hatch ability percentage and promoted early hatch ability and decreased embryonic mortality in treated groups (G1) and (G2) than control (G3). Significant decreases in RBCs counts, cholesterol and total lipids were found in G1 and G2. Exposure of developing embryos to red laser radiation had no significant effect on PCV in one day old hatching chicks, or RBCs and WBCs counts, PCV, GOT, uric acid creatinine in six weeks old birds. At the same time, significant increases in Hb and WBCs counts were occurred in one day old chicks and Hb and GPT in six weeks old birds. It could be concluded from the reported findings that exposure to red laser radiation may affect some factors that accelerate hatch ability and cause changes in some biochemical parameters in Japanese quails

  7. Differential response of biochemical parameters to EMS and MMS treatments and their dose effect relationship on chromosomes in induced diabetic mouse

    Directory of Open Access Journals (Sweden)

    B.B.D. Khalandar

    2015-10-01

    Conclusion: (1 Even though alkylating agents induce chromosomal aberrations in diabetic mice, MMS, a methylating agent is a more potent inducer of chromatid type of aberrations than EMS, an ethylating agent. (2 Diabetic mouse is more resistant than the non diabetic to alkylating agents and (3 the tested agents altered the analyzed biochemical parameters.

  8. Changes of hematological and biochemical parameters revealed genotoxicity and immunotoxicity of neonicotinoids on Chinese rare minnows (Gobiocypris rarus).

    Science.gov (United States)

    Hong, Xiangsheng; Zhao, Xu; Tian, Xue; Li, Jiasu; Zha, Jinmiao

    2018-02-01

    Adverse impacts of immunity in terrestrial non-target organisms exposed to neonicotinoid insecticides have been reported, but the causal link between insecticide exposure and possible immune alterations in fish remains limited. In the present study, the potential genotoxicity and immunotoxicity of three neonicotinoids (imidacloprid, nitenpyram, and dinotefuran) were assessed in Chinese rare minnows by using a 60-day chronic toxicity test. The hematological and biochemical parameters of juvenile Chinese rare minnows and changes in the transcription of six inflammation-related genes were determined after exposure to neonicotinoids at 0.1, 0.5, or 2.0 mg/L. A clear difference in the frequency of erythrocytes with micronuclei (MN) was observed after treatment with 2.0 mg/L imidacloprid (p < .05). Additionally, exposure to 0.5 or 2.0 mg/L imidacloprid significantly increased the binucleated (BN) erythrocytes and those with notched nuclei (NT) (p < .05). A serum protein electrophoresis (SPE) assay showed significant alterations in the serum protein in all treatments (p < .05), and further analysis indicated decreases in immunoglobulin (Ig) in treatments with 0.5 or 2.0 mg/L imidacloprid or dinotefuran or with 0.1 mg/L nitenpyram (p < .05). Moreover, a biochemical assay confirmed that immunoglobulin M (IgM) levels were indeed significantly decreased upon treatment with imidacloprid or dinotefuran at 0.5 or 2.0 mg/L (p < .05). In addition, the transcriptional levels of the inflammatory cytokines IL-6, INF-α, TNF-α, and IL-1β were markedly down-regulated after all imidacloprid treatments (p < .05), whereas the expression levels of only TNF-α and IL-1β were significantly down-regulated following the 0.5 and 2.0 mg/L dinotefuran treatments (p < .05). Taken together, our results clearly demonstrate that imidacloprid, rather than nitenpyram and dinotefuran, can induce genotoxicity. The responsiveness of these immune indicators

  9. Does Cigarette Smoking Affect Seminal Fluid Parameters? A Comparative Study

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    Zakarya Bani Meri

    2013-01-01

    Full Text Available Objective: To study the effect of cigarette smoking on seminal fluid parameters, namely; volume, sperm concentration, and motility, as well as morphology, leukocyte infiltration, among males complaining of infertility.Methods: Between August 2010 and July 2011, seminal fluid analysis was done for 1438 males who are partners of couples who visited the infertility clinic at Prince Rashid Ben Al Hassan Hospital (PRH for infertility. The men who fit the inclusion criteria (n=960 were classified into two groups: group a (non-smokers; n=564 and group B (smokers; n=396, which represents 41.25% of the study group. Seminal fluid was collected using masturbation after 3-5 days of abstinence then analyzed for volume, sperm count, sperm concentration, motility and morphology. In order to analyze whether the number of cigarettes smoked per day has an effect on the spermatogram; the smoking men were divided into two subgroups: the heavy smokers (n=266 and non-heavy smokers (n=130.Results: A total of 960 adult males were enrolled. Their age ranged between 21 and 76 years, 564 were non-smokers with mean age of 36. 45±6.27 (Mean±SD. Three-hundred-and-ninety-six were smokers with a mean age of 34.35±4.25 (Mean±SD. There was a significant effect of smoking on the motility of sperms and the ratios of abnormality (p<0.005. Concentration appeared not to be affected by smoking. Furthermore, the group of heavy smokers were found to have lower sperm concentrations and a higher percentage of abnormal sperms compared to the non-heavy smokers.Conclusion: Cigarette smoking has a deleterious effect on some of the seminal fluid parameters (motility, morphology and leukocyte count which in turn may result in male subfertility.

  10. Silymarin improves the behavioural, biochemical and histoarchitecture alterations in focal ischemic rats: a comparative evaluation with piracetam and protocatachuic acid.

    Science.gov (United States)

    Muley, Milind M; Thakare, Vishnu N; Patil, Rajesh R; Kshirsagar, Ajay D; Naik, Suresh R

    2012-08-01

    Comparative neuroprotective potential of silymarin, piracetam and protocatechuic acid ethyl ester (PCA) was evaluated in focal ischemic rats. Various pharmacological, biochemical (lipid peroxidation, reduced glutathione, catalase, nitrite content, brain water content) and behavioural (memory impairment, motor control, neurological score) including infarct size and histopathological alterations were evaluated. Silymarin (200mg/kg) and PCA treatment significantly improved behavioural, biochemical and histopathological changes, and reduced water content and infarct size. However, piracetam only improved behavioural and histopathological changes, reduced water content and infarct size. The findings indicate that silymarin exhibits neuroprotective activity better than PCA and piracetam in focal ischemia/reperfusion reflected by its better restoration of behavioural and antioxidant profile. Copyright © 2012 Elsevier Inc. All rights reserved.

  11. Evaluation of biochemical and haematological parameters and prevalence of selected pathogens in feral cats from urban and rural habitats in South Korea.

    Science.gov (United States)

    Hwang, Jusun; Gottdenker, Nicole; Min, Mi-Sook; Lee, Hang; Chun, Myung-Sun

    2016-06-01

    In this study, we evaluated the potential association between the habitat types of feral cats and the prevalence of selected infectious pathogens and health status based on a set of blood parameters. We live-trapped 72 feral cats from two different habitat types: an urban area (n = 48) and a rural agricultural area (n = 24). We compared blood values and the prevalence of feline immunodeficiency virus (FIV), feline leukaemia virus (FeLV) and haemotropic Mycoplasma infection in feral cats from the two contrasting habitats. Significant differences were observed in several blood values (haematocrit, red blood cells, blood urea nitrogen, creatinine) depending on the habitat type and/or sex of the cat. Two individuals from the urban area were seropositive for FIV (3.0%), and eight (12.1%) were positive for FeLV infection (five from an urban habitat and three from a rural habitat). Haemoplasma infection was more common. Based on molecular analysis, 38 cats (54.3%) were positive for haemoplasma, with a significantly higher infection rate in cats from rural habitats (70.8%) compared with urban cats (47.8%). Our study recorded haematological and serum biochemical values, and prevalence of selected pathogens in feral cat populations from two different habitat types. A subset of important laboratory parameters from rural cats showed values under or above the corresponding reference intervals for healthy domestic cats, suggesting potential differences in the health status of feral cats depending on the habitat type. Our findings provide information about the association between 1) blood values (haematological and serum biochemistry parameters) and 2) prevalence of selected pathogen infections and different habitat types; this may be important for veterinarians who work with feral and/or stray cats and for overall cat welfare management. © ISFM and AAFP 2015.

  12. Impact of seasonal thermal stress on physiological and blood biochemical parameters in pigs under different dietary energy levels.

    Science.gov (United States)

    Pathak, P K; Roychoudhury, R; Saharia, J; Borah, M C; Dutta, D J; Bhuyan, R; Kalita, D

    2018-06-01

    The present study was formulated to find out the status of important season related thermal stress biomarkers of pure-bred (Hampshire) and crossbred (50% Hampshire × 50% local) pigs under the agro-climatic condition of Assam State, India. The experiment was also aimed to study the role of different level of energy ration (110, 100, and 90% energy of NRC feeding standard for pig) in variation of physiological and biochemical parameters in two genetic groups of pigs in different seasons. The metabolizable energy value were 3260, 2936.5, and 3585.8 kcal/kg in grower ration and 3260.2, 2936.6, and 3587 kcal/kg in finisher ration for normal energy (NE), low energy (LE) and high energy (HE), respectively. Both the genetic group of animals were housed separately under intensive system of management. Each pen was measuring 10' × 12' along with an outer enclosure. Six weaned piglets (almost similar body weight of average 10.55 kg) of each group were kept in a separate pen. However, after attainment of 35 kg body weight, the animals of a group were divided in two pens of three animals each. The present experiment indicated that average ambient temperature during summer months (27.33-29.51 °C) was above the comfort zone for pigs (22 °C). The significantly (P energy (HE) ration during summer season. Serum triiodothyronine (T 3 ) and thyroxine (T 4 ) concentrations were significantly (P energy level of the ration might be helpful to minimize the effects of thermal stress during summer.

  13. Effect of subacute poisoning with bifenthrin on locomotor activity, memory retention, haematological, biochemical and histopathological parameters in mice.

    Science.gov (United States)

    Nieradko-Iwanicka, B; Borzecki, A; Jodlowska-Jedrych, B

    2015-02-01

    Bifenthrin (BIF) is a pyrethroid (PYR) insecticide. The target point for PYR's toxic action are voltage sensitive sodium channels in the central nervous system (CNS). Intoxication with PYRs results in motor activity impairment and death in insects. Although PYRs are considered to be safe for mammals, there were numerous cases of pyrethroid poisoning in humans, animals and pets described. The general population is chronically exposed to PYRs via grain products, dust and indoor air. Therefore new questions arise: whether PYRs act in a dose-additive fashion in the course of subacute poisoning, are there other target organs (but brain) for BIF and if there is one common mechanism of its' toxic action in different organs. The objective of this work was to characterize the effect of BIF at the doses of 4 or 8 mg/kg injected intraperitoneally (i.p.) daily for 28 consecutive days on memory and motor activity, hematological, biochemical and histopathological parameters in mice. BIF at the doses of 8 mg/kg or 4 mg/kg of body mass was administered i.p. daily to the mice for 28 consecutive days. Motor function was measured on day 1, 7, 14 and 28 and memory retention was tested in a passive avoidance task on day 2, 7, 14 and 28. BIF significantly impaired memory retention on day 2. BIF decreased locomotor activity at every stage of the experiment in a single dose depending manner. No behavioral cumulative effect was observed. Subacute poisoning with the higher dose of BIF caused anaemia, elevated white blood cell count (WBC), elevated alanine transaminase (ALT), superoxide dismuthase (SOD), and decreased glutathione peroxidase (GPx) activity. Lymphocyte infiltrates were visualized in the livers. subacute poisoning with BIF decreases locomotor activity in a single dose proportionate manner. BIF damages also the liver and alters blood morphology. The possible common mechanism of these effects can be oxidative stress.

  14. Effect of Ethanolic Extract of Emblica officinalis on Histopathology of Kidney and on Biochemical Parameters in Hyperlipidemic Albino Rats

    Directory of Open Access Journals (Sweden)

    Bheemshetty S. Patil

    2015-01-01

    Full Text Available Background: It has been reported that hyperlipidemia plays a central role in the development of atherosclerosis and oxidative stress. Embilica officinalis also known as Amla or Indian Gooseberry acts as antihyperlipidemic and antioxidant. Its active ingredients contains tannins, gallic acid and flavonoids. Aim & Objectives: It was aimed to evaluate the effect of ethanolic extract of Emblica officinalis on histopathology of kidney and on biochemical parameters in hyperlipidemic albino Wistar rats. Material and Methods: Extraction of dried fruits of Emblica officinalis was done by Soxhlet apparatus 0 using 99% ethanol at 60 C for 24 hours and also phytochemical analysis was done. Group I served as normal control. Group II was fed with isocaloric diet. Group III was fed with hyperlipidemic diet. Group IV was fed with isocaloric diet for 21 days + Embilica officinalis for 21 days. Group V was fed with hyperlipidemic diet for 21 days+ Embilica officinalis for 21 days. The dose of ethanolic extract of Emblica officinalis was taken as 100mg/kg body weight daily. Results: Percent body weight gain, kidney weight and nephro-somatic index significantly improved in hyperlipidemic rats treated with Emblica officinalis. There was a significant improvement in serum electrolyte and kidney markers. It was found that there were focal glomerular lesions with thickening of glomerulus in the kidneys of rats on hyperlipidemic diet and normal renal histology of rats on hyperlipidemic diet treated with Emblica officinalis. Conclusion: It can be concluded that Emblica officinalis may be a good, natural therapeutic agent against hyperlipidemic diet induced oxidative damage and nephrotoxicity.

  15. Effects of dietary level of tannic acid and protein on internal organ weights and biochemical blood parameters of rats.

    Directory of Open Access Journals (Sweden)

    Marcin Barszcz

    Full Text Available Tannic acid (TA is a polyphenolic compound with a health-promoting potential for humans. It is hypothesised that TA effects on the relative weight of internal organs and biochemical blood indices are modified by dietary protein level in rats. The study involved 72 rats divided into 12 groups fed diets with 10 or 18% of crude protein (CP and supplemented with 0, 0.25, 0.5, 1, 1.5 or 2% of TA. After 3 weeks of feeding, the relative weight of the caecum was greater in rats fed TA diets, while feeding diets with 10% of CP increased the relative weight of the stomach, small intestine and caecum, but decreased that of kidneys and spleen. Albumin concentration was higher in rats fed 0.25% and 0.5% TA diets than in rats given the 2% TA diets. The 2% TA diets reduced creatine kinase (CK activity compared to non-supplemented diets and those with 0.5, 1 and 1.5% of TA. Rats fed the 10% CP diets had a higher activity of alkaline phosphatase, amylase, and γ-glutamyltransferase as well as the concentration of iron and cholesterol, but lower that of urea and uric acid. The interaction affected only cholinesterase activity. In conclusion, TA induced caecal hypertrophy and could act as a cardioprotective agent, as demonstrated by reduced CK activity, but these effects were not modified by dietary protein level.

  16. Effect of dystocia on some hormonal and biochemical parameters in the one-humped camel (Camelus dromedarius).

    Science.gov (United States)

    Ghoneim, I M; Waheed, M M; Al-Eknah, M M; Al-Raja'a, A

    2016-08-01

    The present study compared some of the hormonal and biochemical constituents of serum from eutocic and dystocic one-humped camels (Camelus dromedarius). Sera were harvested from eutocic (n = 9) and dystocic (n = 20) camels within the first 15 minutes after delivery. Although there were no differences in the concentrations of estradiol-17β (E2) and prostaglandin F2α (PGF2α) between the eutocic and the dystocic animals, the level of progesterone (P4) and cortisol was significantly higher (P dystocia than those that had a normal birth. There were no differences between the concentrations of alkaline phosphatase, aspartate aminotransferase, calcium, cholesterol, creatine kinase, creatinine, or magnesium (Mg) in eutocic and dystocic animals. The nitric oxide concentration was significantly higher (P dystocia than those that had normal births. By contrast, the serum concentrations of glucose, phosphorus (P), and triglycerides were significantly lower (P dystocia. Moreover, stress and hormonal changes may affect the metabolic traits in dystocia camels. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. A comparative clinical, pathological, biochemical and genetic study of fused in sarcoma proteinopathies

    DEFF Research Database (Denmark)

    Lashley, Tammaryn; Rohrer, Jonathan D; Bandopadhyay, Rina

    2011-01-01

    Neuronal intermediate filament inclusion disease and atypical frontotemporal lobar degeneration are rare diseases characterized by ubiquitin-positive inclusions lacking transactive response DNA-binding protein-43 and tau. Recently, mutations in the fused in sarcoma gene have been shown to cause...... findings, as well as genetic and biochemical data in 14 fused in sarcoma proteinopathy cases. In this cohort, the age of onset was variable but included cases of young-onset disease. Patients with atypical frontotemporal lobar degeneration with ubiquitinated inclusions all presented with behavioural...... familial amyotrophic lateral sclerosis and fused in sarcoma-positive neuronal inclusions have subsequently been demonstrated in neuronal intermediate filament inclusion disease and atypical frontotemporal lobar degeneration with ubiquitinated inclusions. Here we provide clinical, imaging, morphological...

  18. Measuring recovery: An adapted Brief Assessment of Mood (BAM+) compared to biochemical and power output alterations.

    Science.gov (United States)

    Shearer, David A; Sparkes, William; Northeast, Jonny; Cunningham, Daniel J; Cook, Christian J; Kilduff, Liam P

    2017-05-01

    Biochemical (e.g. creatine kinase (CK)) and neuromuscular (e.g. peak power output (PPO)) markers of recovery are expensive and require specialist equipment. Perceptual measures are an effective alternative, yet most validated scales are too long for daily use. This study utilises a longitudinal multi-level design to test an adapted Brief Assessment of Mood (BAM+), with four extra items and a 100mm visual analogue scale to measure recovery. Elite under-21 academy soccer players (N=11) were monitored across five games with data (BAM+, CK and PPO) collected for each game at 24h pre, 24h and 48h post-match. Match activity data for each participant was also collected using GPS monitors on players. BAM+, CK and PPO had significant (pathletes. Future research is needed to confirm both the scales reliability and validity. Copyright © 2016 Sports Medicine Australia. Published by Elsevier Ltd. All rights reserved.

  19. Comparative study of human blood Raman spectra and biochemical analysis of patients with cancer

    Science.gov (United States)

    Shamina, Lyudmila A.; Bratchenko, Ivan A.; Artemyev, Dmitry N.; Myakinin, Oleg O.; Moryatov, Alexander A.; Orlov, Andrey E.; Kozlov, Sergey V.; Zakharov, Valery P.

    2018-04-01

    In this study we measured spectral features of blood by Raman spectroscopy. Correlation of the obtained spectral data and biochemical studies results is investigated. Analysis of specific spectra allows for identification of informative spectral bands proportional to components whose content is associated with body fluids homeostasis changes at various pathological conditions. Regression analysis of the obtained spectral data allows for discriminating the lung cancer from other tumors with a posteriori probability of 88.3%. The potentiality of applying surface-enhanced Raman spectroscopy with utilized experimental setup for further studies of the body fluids component composition was estimated. The greatest signal amplification was achieved for the gold substrate with a surface roughness of 1 μm. In general, the developed approach of body fluids analysis provides the basis of a useful and minimally invasive method of pathologies screening.

  20. Assessment of the Safety of the Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes), in Rats: Biochemical, Hematological, and Antioxidative Parameters.

    Science.gov (United States)

    Grotto, Denise; Bueno, Daiane Cristovam Rosa; Ramos, Gabriela Karine de Almeida; da Costa, Susi Rosa; Spim, Sara Rosicler Vieira; Gerenutti, Marli

    2016-01-01

    Lentinus edodes is an edible mushroom studied for use, or as an adjunct, in the prevention of illnesses such as hypertension, hypercholesterolemia, diabetes, and cancer. Despite the functional properties of L. edodes, the doses commonly reported in experimental studies are much higher than those actually consumed. Thus, we aimed to establish the optimum intake levels of L. edodes in vivo. Four groups of male Wistar rats received dry and powdered L. edodes reconstituted in water for 30 days: control (water only), L. edodes 100 mg/kg, L. edodes 400 mg/kg, and L. edodes 800 mg/kg. Biochemical and hematological parameters were assessed using commercial kits. Antioxidant parameters were quantified spectrophotometrically. Neither cholesterol, triglycerides, glucose, nor transaminase activity was different among any of the L. edodes concentrations. However, fructosamine concentrations were significantly decreased in groups consuming L. edodes at 100 or 400 mg/kg. A significant decrease in hemoglobin concentration was found in the 400 and 800 mg/kg/day L. edodes groups, and leukopenia occurred in rats that consumed L. edodes 800 mg/kg/day compared with the control group. L. edodes at 100 and 400 mg/kg increased amounts of reduced glutathione compared with the control group. L. edodes was effective as an antioxidant at 100 and 400 mg/kg, but at 400 and 800 mg/kg some disturbances were observed, such as reductions in hemoglobin and leukocytes. In summary, this study has potential benefits for scientific development because the safe daily intake of L. edodes (at 100 mg/kg) is, to our knowledge, reported for the first time in a preclinical study.

  1. pH stability and comparative evaluation of ranaspumin-2 foam for application in biochemical reactors

    International Nuclear Information System (INIS)

    Choi, Hyo-Jick; Ebersbacher, Charles F; Quan, Fu-Shi; Montemagno, Carlo D

    2013-01-01

    Aqueous channels of foam represent a simplified, natural bioreactor on the micro-/nano-scale. Previous studies have demonstrated the feasibility and potential application of foams in replicating cellular process in vitro, but no research has been performed to establish a basis for designing stable and biocompatible foam formulations. Our research has been directed specifically to the evaluation of ranaspumin-2 (RSN-2), a frog foam nest protein. The strong surfactant activity of RSN-2 enabled us to produce foams using low protein concentration (1 mg ml −1 ) over a wide pH range (pH ≥ 3). Importantly, the RSN-2 formulation exhibited the best foam stability at a near neutral pH condition, which shows a potential for application to various biosynthesis applications. Model cellular systems such as liposomes and inactivated A/PR/8/34 influenza virus maintained their physicochemical stability and full hemagglutination activity, indicating biocompatibility of RSN-2 with both cellular membranes and proteins both in bulk solution and in foam. Moreover, the addition of RSN-2 did not exert any deteriorative effects on bacterial cell growth kinetics. In contrast, Tween 20, Triton X-100, and BSA did not show satisfactory performance in terms of foamability, foam stability, physicochemcial stability, and biochemical stability. Although our study has been limited to representative formulations composed of only surfactant molecules, a number of unique advantages make RSN-2 a promising candidate for in vitro foam biosynthesis. (paper)

  2. Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae

    Directory of Open Access Journals (Sweden)

    Juliana Castro Monteiro

    2009-04-01

    Full Text Available Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT, alanine transaminase (ALT, aspartate transaminase (AST, chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG occurred in the group that received the higher dose.As folhas de Rudgea viburnoides Benth. são utilizadas na medicina popular como diuréticas, hipotensoras, anti-reumáticas, depurativas do sangue e em regimes de emagrecimento. O presente estudo foi delineado para avaliar o efeito da infusão das folhas de R. viburnoides nos parâmetros bioquímicos plasmáticos e na morfologia e morfometria hepática de ratos Wistar adultos. Dois grupos receberam a infusão das folhas, diariamente, nas dosagens de 10 e 20 g de folhas secas/L de água, durante 40 dias. O grupo controle recebeu a mesma quantidade de água. As análises histopatológicas não mostraram áreas degeneradas e infiltrados inflamatórios. A morfometria hepática mostrou acúmulo significativo de gordura nos hepatócitos dos animais tratados, principalmente no grupo que recebeu a maior dose da infusão (8,75% de gotículas lipídicas, comparado com 0,25% delas encontradas nos animais controles. Não foram observadas alterações nos níveis plasmáticos de uréia, creatinina,

  3. The usefulness of whole body bone mineral densitometry in the osteopenia of preterm infants: comparison with the wrist radiography and biochemical parameters

    International Nuclear Information System (INIS)

    Cheon, Bong Jin; Huh, Jin Do; Shin, Sang Bum; Cheon, Byung Kook; Joh, Young Duk; Kwon, Jeong Mi; Jeon, Seong Sook

    1997-01-01

    To evaluate the usefulness of whole body bone mineral densitometry in the diagnosis of frequent osteopenia of preterm infants by comparison with the wrist radiographs and biochemical parameters. From January 1995 to January 1996, we obtained whole body bone mineral density(BMD) studies using dual energy X-ray absorptiometry(DXA) and wrist radiographs of 39 preterm infants. They were divided into three groups according to birth weight, under 1500g, 1501g to 2000g and above 2000g, and four grades of skeletal change, as seen on wrist radiography, according to the scoring method of Koo et al. Groups of birth weight and grades of skeletal change were then correlated with whole body BMD and biochemical parameters. For comparison, normal data were obtained from 13 infants born at full term. Data were analyzed by one way analysis of variation(ANOVA) and correlation and regression analysis. A p-value of less than 0.05 was considered significant. Whole body BMDs were significantly lower in the more premature and smaller birth weight infants(r=0.77, p=0.0000), and in the higher grade of skeletal change(r-0.5276, p=0.0000). Aggravated skeletal changes were found in infants with lower birth weight(r= -0.3822, p=0.01). Interobserver variation in grading skeletal change was 42.9%, and intraobserver variation was 18.4%. Biochemical parameters such as serum calcium, phosphate, alkaline phosphatase, parathromone, calcitonin and 25-hydroxy-vitamine D did not vary significantly according to either birth weight or skeletal change(p > 0.05). Premature osteopenia is more effectively diagnosed by measuring whole body BMD using DXA than by grading radiographical skeletal change or by biochemical parameters

  4. The usefulness of whole body bone mineral densitometry in the osteopenia of preterm infants: comparison with the wrist radiography and biochemical parameters

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, Bong Jin; Huh, Jin Do; Shin, Sang Bum; Cheon, Byung Kook; Joh, Young Duk [Kosin Univ. Gospel Hospital, Pusan (Korea, Republic of); Kwon, Jeong Mi; Jeon, Seong Sook [Ilsin Christian Hospital, Pusan (Korea, Republic of)

    1997-02-01

    To evaluate the usefulness of whole body bone mineral densitometry in the diagnosis of frequent osteopenia of preterm infants by comparison with the wrist radiographs and biochemical parameters. From January 1995 to January 1996, we obtained whole body bone mineral density(BMD) studies using dual energy X-ray absorptiometry(DXA) and wrist radiographs of 39 preterm infants. They were divided into three groups according to birth weight, under 1500g, 1501g to 2000g and above 2000g, and four grades of skeletal change, as seen on wrist radiography, according to the scoring method of Koo et al. Groups of birth weight and grades of skeletal change were then correlated with whole body BMD and biochemical parameters. For comparison, normal data were obtained from 13 infants born at full term. Data were analyzed by one way analysis of variation(ANOVA) and correlation and regression analysis. A p-value of less than 0.05 was considered significant. Whole body BMDs were significantly lower in the more premature and smaller birth weight infants(r=0.77, p=0.0000), and in the higher grade of skeletal change(r-0.5276, p=0.0000). Aggravated skeletal changes were found in infants with lower birth weight(r= -0.3822, p=0.01). Interobserver variation in grading skeletal change was 42.9%, and intraobserver variation was 18.4%. Biochemical parameters such as serum calcium, phosphate, alkaline phosphatase, parathromone, calcitonin and 25-hydroxy-vitamine D did not vary significantly according to either birth weight or skeletal change(p > 0.05). Premature osteopenia is more effectively diagnosed by measuring whole body BMD using DXA than by grading radiographical skeletal change or by biochemical parameters.

  5. Analysis of the Variability of Epstein-Barr Virus Genes in Infectious Mononucleosis: Investigation of the Potential Correlation with Biochemical Parameters of Hepatic Involvement

    Directory of Open Access Journals (Sweden)

    Banko Ana

    2016-09-01

    Full Text Available Background: Primary Epstein-Barr virus (EBV infection is usually asymptomatic, although at times it results in the benign lymphoproliferative disease, infectious mononucleosis (IM, during which almost half of patients develop hepatitis. The aims of the present study are to evaluate polymorphisms of EBV genes circulating in IM isolates from this geographic region and to investigate the correlation of viral sequence patterns with the available IM biochemical parameters.

  6. Determination of the Effect of the Elimination Diet Applied for Overweight and Obese People with Food Intolerance on Body Composition and Biochemical Parameters

    Directory of Open Access Journals (Sweden)

    Sema Gubur

    2017-08-01

    Full Text Available ABSTRACT Food Intolerance, is a reaction against food, but not immunological manner, and may be confused with real food allergies. In this study, effects of special weight-loss diet together with an elimination diet on body composition and biochemical parameters of overweight and obese people who were diagnosed with food intolerance were investigated. The study group consists of 20 patients in total who were followed-up and treated in Yorktest Turkey Laboratory; who were diagnosed with food intolerance, and whose BMI was > 26kg/m2. Bloodletting for these patients was executed with Lancet from their fingertips, and the blood drawn from these patients was assessed via enzyme-linked immunosorbent assay (ELİSA method, and food reactions of patients were determined for each food. Biochemical parameters of these patients are routine tests, which are necessary for food intolerance tests, and they are analyzed at Yorktest Turkey Laboratory for two times: before and after elimination diet plus special weight-loss diet. It has been determined that, the most common sensivity is obtained against yeast, egg yolk and white, cranberry, cow’s milk, chicken, lentils and parsley. Anthropometric measurements and biochemical parameters before and after elimination diet plus special weight-loss diet applied to participants, were significantly improved in statistical manner. Due to positive changes in body composition and biochemical parameters obtained through application of special weight-loss diets together with elimination diet applied to fat and obese people, we think that this diet might be used for medical nutrition treatment of obesity as a treatment option.

  7. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats.

    Science.gov (United States)

    Beck, W; Gobatto, C

    2016-03-01

    Nocturnal rodents should be assessed at an appropriate time of day, which leads to a challenge in identifying an adequate environmental light which allows animal visualisation without perturbing physiological homeostasis. Thus, we analysed the influence of high wavelength and low intensity light during dark period on physical exercise and biochemical and haematological parameters of nocturnal rats. We submitted 80 animals to an exhaustive exercise at individualised intensity under two different illuminations during dark period. Red light (> 600 nm; sports performance experiments.

  8. Protective effect of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide.

    Science.gov (United States)

    Aghaei, S; Nikzad, H; Taghizadeh, M; Tameh, A A; Taherian, A; Moravveji, A

    2014-10-01

    Cancer treatment with cyclophosphamide (CP) may result in reproductive toxicity as one of its side effects. The pumpkin seed is a rich natural source of antioxidant. We have assessed the possible protective efficacy of pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology of CP-treated rats. Male adult Wistar rats were categorised into four groups. Group 1 served as control and received intraperitoneal (IP) injection of isotonic saline solution. Group 2 rats were treated with CP by IP injection in a single dose of 100 mg/kg body weight, only once. Group 3 and 4 received CP plus 300 and 600 mg/kg pumpkin seed extract respectively. Six weeks after treatment, sperm characteristics, biochemical parameters and histopathological changes were examined. Results showed that, sperm characteristics in CP-treated rats were significantly decreased. Biochemical analysis results showed that the co-administration of 300 mg pumpkin seed extract could increase the total antioxidant capacity (TAC) level significantly. In CP-treated rats, histopathological changes such as vacuolisation, disorganisation and separation of epididymal epithelium were observed as well. Interestingly, pumpkin seed extract could improve the above-mentioned parameters remarkably in CP-treated rats. Our findings indicated that pumpkin seed extract might be used as protective agent against CP-induced reproductive toxicity. © 2013 Blackwell Verlag GmbH.

  9. Comparison of Selected Morphological, Rheological and Biochemical Parameters of Winter Swimmers' Blood at the End of One Winter Swimming Season and at the Beginning of Another.

    Science.gov (United States)

    Teległów, Aneta; Marchewka, Jakub; Tabarowski, Zbigniew; Rembiasz, Konrad; Głodzik, Jacek; Scisłowska-Czarnecka, Anna

    2015-01-01

    The aim of the study was to examine potential differences in the morphological, rheological and biochemical blood parameters of winter swimmers who remained physically active during the period between the end of one winter swimming season and the beginning of another. The study included a group of healthy winter swimmers (n = 17, all between 30 and 60 years of age). Six months following the end of winter season, the levels of mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin turned out to be significantly higher, while erythrocyte count and hematocrit level significantly lower than at the baseline. Moreover, the break in winter swimming was reflected by a significant increase in median erythrocyte elongation index at all shear stress levels ≥ 1.13 Pa. The only significant changes in biochemical parameters of the blood pertained to an increase in the concentration of transferrin and to a decrease in the total protein, albumin and beta-1 globulin concentrations. Seasonal effort of winter swimmers between the end of one winter swimming season and the beginning of another has a positive influence on morphological, rheological and biochemical blood parameters.

  10. PROPOSED MODIFICATIONS OF K2-TEMPERATURE RELATION AND LEAST SQUARES ESTIMATES OF BOD (BIOCHEMICAL OXYGEN DEMAND) PARAMETERS

    Science.gov (United States)

    A technique is presented for finding the least squares estimates for the ultimate biochemical oxygen demand (BOD) and rate coefficient for the BOD reaction without resorting to complicated computer algorithms or subjective graphical methods. This may be used in stream water quali...

  11. Tumor features and correlation between lymphocyte count and biochemical parameters in patients with hepatitis B virus-associated primary liver cancer with Yin deficiency

    Directory of Open Access Journals (Sweden)

    YANG Zhiyun

    2016-03-01

    Full Text Available ObjectiveTo investigate the tumor features and the correlation between lymphocyte count and biochemical parameters in patients with hepatitis B virus-associated primary liver cancer (PLC with yin deficiency. MethodsA total of 148 PLC patients who were treated in Beijing Ditan Hospital, Capital Medical University, from July 2013 to February 2015 were enrolled and divided into yin-deficiency PLC group (52 patients and non-yin-deficiency PLC group (96 patients. The patients′ general information and laboratory markers were collected, including oncological parameters (alpha-fetoprotein, carcinoembryonic antigen (CEA, and carbohydrate antigen 199 (CA19-9, virological parameter (HBsAg, gross type (nodular type, massive type, bulky type, and diffuse type, radiological features (main portal vein diameter, portal vein tumor thrombus, and extrahepatic metastasis, biochemical parameters (Model for End-Stage Liver Disease (MELD score, white blood cell, red blood cell, platelet (PLT, alanine aminotransferase, aspartate aminotransferase, total bilirubin (TBil, gamma-glutamyl transpeptidase, alkaline phosphatase, albumin, cholinesterase, prothrombin time (PT, and prothrombin time activity (PTA, and lymphocyte count. The t-test was applied for comparison of normally distributed continuous data between groups, and the Pearson correlation analysis was applied for correlation analysis. The Mann-Whitney U test was applied for comparison of non-normally distributed continuous data between groups, and the Spearman correlation analysis was applied for correlation analysis. The chi-square test was applied for comparison of categorical data between groups. ResultsHBsAg showed a significant difference between the two groups (χ2=5.658, P=0.017. Compared with the non-yin-deficiency PLC group, the yin-deficiency PLC group had significantly increased CEA and CA19-9 (U=-2.200 and -2.194, both P<0.05, significantly increased MELD score, TBil, and PT (t=2.2, U=-2.0, U=-2

  12. Biochemical responses of filamentous algae in different aquatic ecosystems in South East Turkey and associated water quality parameters.

    Science.gov (United States)

    Çelekli, Abuzer; Arslanargun, Hamdullah; Soysal, Çiğdem; Gültekin, Emine; Bozkurt, Hüseyin

    2016-11-01

    To the best of our knowledge, any study about biochemical response of filamentous algae in the complex freshwater ecosystems has not been found in the literature. This study was designed to explore biochemical response of filamentous algae in different water bodies from May 2013 to October 2014, using multivariate approach in the South East of Turkey. Environmental variables were measured in situ: water temperature, oxygen concentration, saturation, conductivity, salinity, pH, redox potential, and total dissolved solid. Chemical variables of aqueous samples and biochemical compounds of filamentous algae were also measured. It was found that geographic position and anthropogenic activities had strong effect on physico-chemical variables of water bodies. Variation in environmental conditions caused change in algal biomass composition due to the different response of filamentous species, also indicated by FTIR analysis. Biochemical responses not only changed from species to species, but also varied for the same species at different sampling time and sampling stations. Multivariate analyses showed that heavy metals, nutrients, and water hardness were found as the important variables governing the temporal and spatial succession and biochemical compounds. Nutrients, especially nitrate, could stimulate pigment and total protein production, whereas high metal content had adverse effects. Amount of malondialdehyde (MDA), H2O2, total thiol groups, total phenolic compounds, proline, total carbohydrate, and metal bioaccumulation by filamentous algae could be closely related with heavy metals in the ecosystems. Significant increase in MDA, H2O2, total thiol group, total phenolic compounds, and proline productions by filamentous algae and chlorosis phenomenon seemed to be an important strategy for alleviating environmental factors-induced oxidative stress as biomarkers. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Comparative biochemical composition in gonad and adductor muscle of triploid and diploid catarina scallop (Argopecten ventricosus Sowerby II, 1842).

    Science.gov (United States)

    Ruiz-Verdugo, C A.; Racotta, I S.; Ibarra, A M.

    2001-05-15

    Biochemical components of gonad and adductor muscle for diploid and triploid catarina scallop, Argopecten ventricosus, were evaluated and compared at four periods in 1 year (January, April, June, and October). Two comparisons were done. The first one compared an untreated control (diploid) vs. a triploidy-treated group for which the percentage of triploids was 57%. The second comparison was done on a group derived from within the triploidy-treated group, separating diploids (internal control) from triploids ('putative triploids'). Regardless of which comparison, in the gonad diploid scallops had larger concentrations of proteins, carbohydrates, lipids, and acylglycerols than triploid scallops. This reflects the maturation processes in diploid scallops vs. the sterility seen in most triploid scallops, and it is particularly supported by the consistently larger concentration of acylglycerols in gonads of diploids than in triploids. The gonad index of the internal control (diploid) group was significantly larger than that seen in the putative triploid group at all sampling periods but October, when none of the gonad biochemical components were different between ploidy groups.Triploid scallops had a significantly larger muscle index than diploids from April to October. This can be caused by a larger gain in muscle tissue in triploids than diploids from January to June. However, there were no consistent differences in any of the biochemical components evaluated in adductor muscle of diploids and triploids. The use of freshly ingested food rather than reserve mobilization from muscle in diploids is suggested by these results. Nutrients derived from ingested food are apparently used for muscle growth in triploids, whereas in diploids those nutrients serve primarily for gonad development. The importance of freshly ingested food for maintenance and growth is suggested because the decline in biochemical components seen in October in both muscle and gonad was paired with a

  14. Comparative study of radiation shielding parameters for bismuth borate glasses

    International Nuclear Information System (INIS)

    Kaundal, Rajinder Singh

    2016-01-01

    Melt and quench technique was used for the preparation of glassy samples of the composition x Bi 2 O 3- (1-x) B 2 O 3 where x= .05 to .040. XCOM computer program is used for the evaluation of gamma-ray shielding parameters of the prepared glass samples. Further the values of mass attenuation coefficients, effective atomic number and half value layer for the glassy samples have been calculated in the energy range from 1KeV to 100GeV. Rigidity of the glass samples have been analyzed by molar volume of the prepared glass samples. (author)

  15. Comparative study of radiation shielding parameters for bismuth borate glasses

    Energy Technology Data Exchange (ETDEWEB)

    Kaundal, Rajinder Singh, E-mail: rajinder_apd@yahoo.com [Department of Physics, School of Physical Sciences, Lovely Professional University, Phagwara, Punjab (India)

    2016-07-15

    Melt and quench technique was used for the preparation of glassy samples of the composition x Bi{sub 2}O{sub 3-}(1-x) B{sub 2}O{sub 3} where x= .05 to .040. XCOM computer program is used for the evaluation of gamma-ray shielding parameters of the prepared glass samples. Further the values of mass attenuation coefficients, effective atomic number and half value layer for the glassy samples have been calculated in the energy range from 1KeV to 100GeV. Rigidity of the glass samples have been analyzed by molar volume of the prepared glass samples. (author)

  16. Comparative Study of Radiation Shielding Parameters for Bismuth Borate Glasses

    OpenAIRE

    Kaundal, Rajinder Singh

    2016-01-01

    Melt and quench technique was used for the preparation of glassy samples of the composition x Bi2O3-(1-x) B2O3 where x= .05 to .040. XCOM computer program is used for the evaluation of gamma-ray shielding parameters of the prepared glass samples. Further the values of mass attenuation coefficients, effective atomic number and half value layer for the glassy samples have been calculated in the energy range from 1KeV to 100GeV. Rigidity of the glass samples have been analyzed by molar volume of...

  17. Comparison of absolute biochemical parameters of undisturbed soils in Mediterranean environments (NE Spain) with corresponding parameters relative to soil organic carbon

    OpenAIRE

    Jiménez de Ridder, Patrícia; Marando, Graciela; Josa March, Ramon; Ginovart Gisbert, Marta; Ras Sabido, Antoni; Bonmati Pont, Manuel

    2017-01-01

    The study of soil quality requires the establishment of quality standards. To this end, several authors have highlighted the need to create databases of quality indicators, such as biochemical properties, for different types of undisturbed soils under various climates and to establish standardised methodologies for their development. In Spain, studies of the quality of native soils were initiated > 15 years ago by several groups of authors from differing locations, but little is known regardi...

  18. Relationship of tumor necrosis factor alpha genotypes with various biochemical parameters of normal, over weight and obese human subjects

    International Nuclear Information System (INIS)

    Raza, M.; Chaudhary, B.; Shakoori, A.R.

    2008-01-01

    Tumor Necrosis Factor (TNF-alpha) is expressed primarily in adipocytes and elevated levels of this cytokine have been associated with obesity. The purpose of this investigation was to test whether the TNF-alpha -308 polymorphism were associated with insulin resistance or obesity related traits in non-diabetic and diabetic patients visiting Sheikh Zayed Hospital, Lahore, Fatima Hospital and Irfan Clinic in Sargodha. In non diabetic subjects the AA allele carriers, compared with homozygous G allele carriers had significantly lower (28%) triglyceride values and 15% higher HDL yal ues, whereas other parameters tested 81id not show any significant variation. In diabetic patients the AA allele carriers, compared with GG allele carriers, besides having 31 % higher FBS and 26% higher creatinine, had 20% higher cholesterol and 34% higher triglycerides. The HDL values were 14% less, compared to GG allele carriers. In normal subjects (BMI 22.85:1:0.25 kgim2), the AA allele carriers showed 132%, 125%, 65% and 112% higher triglycerides, cholesterol and LDL values compared with GG allele carriers. The HDL and creatinine did not show any significant change. In the overweight subjects (BMI: 27.17+-0.17 kgim/sup 2/) all these values were lower than in AA allele carriers compared with GG allele carriers. The AA allele carries had FBS, triglycerides, cholesterol and LDL 28%, 48%, 14% and 14% lower than in the GG allele' carriers, respectively. In obese subjects, (BMI: 36.73+-0.78kgm/sup 2/), however, the FBS, triglycerides, cholesterol and creatinine values were 5%, 8%, 7% and 14% higher in AA allele carries compared to GG allele carriers, respectively. The LDL content was 8% lower in AA allele carrier as compared with the respective GG allele carriers, It is concluded that replacement of G at -308 with A leads to reduced risk for cardiovascular disease in non-diabetic subject, whereas in diabetic patients this mutation-increases the risk of CVD. Using BMI as index of obesity, it was

  19. Adding of ascorbic acid to the culture medium influences the antioxidant status and some biochemical parameters in the hen granulosa cells.

    Science.gov (United States)

    Capcarova, M; Kolesarova, A; Kalafova, A; Bulla, J; Sirotkin, A V

    2015-07-01

    The aim of the present study was to determine the activity of superoxide dismutase (SOD), total antioxidant status (TAS) of the hen granulosa cells, and selected biochemical parameters, including calcium, phosphorus, sodium, potassium, glucose, cholesterol, proteins, in the culture medium of granulosa cells after exposing them to ascorbic acid in vitro conditions. Ovarian granulosa cells of hens were incubated with various doses of ascorbic acid (E1 0.09 mg/ml, E2 0.13 mg/ml, E3 0.17 mg/ml, E4 0.33 mg/ml, E5 0.5 mg/ml). Ascorbic acid did not manifest antioxidant potential and higher doses of ascorbic acid (0.17; 0.33 and 0.5 mg/ml) decreased the activity of SOD in granulosa cells. Vitamin application resulted in a significantly (pascorbic acid might be involved in the regulation of selected biochemical and physiological processes in ovarian granulosa cells.

  20. Changes in biochemical parameters of oral fluid in patients during the orthodontic treatment with a bracket system under the action of a developed mucosal gel with probiotic.

    Science.gov (United States)

    Voronkova, Anna V; Smaglyuk, Lyubov V

    2018-01-01

    Introduction: Many research studies involving orthodontic patients focus on changes in levels of oral microbiocenosis after bracket placement. Based upon this the objective of the current study was to determine the effect of the developed mucosal gel with probiotics on the biochemical parameters of the oral fluid of patients during the orthodontic treatment with a bracket system. The aim: Aim of our study is to determine the effect of the developed mucosal gel with probiotics on the biochemical parameters of the oral fluid of patients during the orthodontic treatment with a bracket system. Materials and methods: 45 patients at the age of 18-24, with 15 people in each group (control, main and comparison group) were examined. The main group was presented by patients who, in order to prevent dysbiosis of the oral cavity during orthodontic treatment, were prescribed local use of the developed mucosal gel with probiotic. The statistical processing of the results of the study was carried out using methods of variation statistics using the EXCEL program (the standard package of Microsoft Office). Results: According to the results of biochemical studies, it was found that the use of orthodontic treatment of mucosal gel with probiotic in patients with crowded teeth contributes to the strengthening of antioxidant protection, an increase in nonspecific resistance, decrease in inflammation and normalization of microbiocenosis of the oral cavity. Conclusion: These studies indicated that the use of the developed mucosal gel with probiotic in patients with maxillofacial anomalies from the first day after fixation, as indicated by the level of biochemical markers of inflammation.

  1. Comparative evaluation of qigong on various parameters of saliva

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    Bayat Movahed S.

    2007-05-01

    Full Text Available Background and Aim: Qigong is a type of Chinese psychosomatic exercise that integrates meditation, slow physical movements, and breathing. Numerous physical and mental benefits have been classically ascribed to qigong. On the other hand, unstimulated saliva is thought to play an important role in oral immunity, enamel stability and moisturizing the oral mucous membrane. Impaired salivary secretion leads to dental caries, mucosal deterioration and mouth dryness. The aim of the present study was to analyze the effect of a qigong program on various parameters of saliva such as quantity of unstimulated saliva, pH and SIgA. Materials and Methods: Twenty three subjects participated in this clinical trial study. The experimental subjects underwent a qigong training program, conducted by a qualified instructor. The program consisted of half an hour daily practice for 6 months (spring and winter. Saliva was collected in two periods: once during the spring before the experiment commencement and the second, in the following spring. During each period saliva collection was done on tuesday of each week. pH and quantity of salvia measurements were taken simultaneously. SIgA measurements were also taken based on the values obtained in the last phase of the experiment at the end of each spring. The results were analyzed using paired sample T test, one way repeated measure and Bon Ferroni multiple comparison. P<0.05 was the level of significance. Results: Based on our findings, the change in the amount of unstimulated salvia as well as SIgA was statistically significant (P<0.001; however, there was no significant difference in pH values before and after experiment. Conclusion: These findings demonstrate that after 6 months of practicing qigong, significant changes in amount of unstimulated saliva and SIgA occurred in participants. The authors suggest that qigong may be a beneficial adjunctive treatment that enhances amount of unstimulated saliva and SIgA.

  2. Lecithin-coated gold nanoflowers (GNFs) for CT scan imaging applications and biochemical parameters; in vitro and in vivo studies.

    Science.gov (United States)

    Aziz, Farooq; Bano, Khizra; Siddique, Ahmad Hassan; Bajwa, Sadia Zafar; Nazir, Aalia; Munawar, Anam; Shaheen, Ayesha; Saeed, Madiha; Afzal, Muhammad; Iqbal, M Zubair; Wu, Aiguo; Khan, Waheed S

    2018-01-09

    We report a novel strategy for the fabrication of lecithin-coated gold nanoflowers (GNFs) via single-step design for CT imaging application. Field-emission electron microscope confirmed flowers like morphology of the as-synthesized nanostructures. Furthermore, these show absorption peak in near-infrared (NIR) region at λ max 690 nm Different concentrations of GNFs are tested as a contrast agent in CT scans at tube voltage 135 kV and tube current 350 mA. These results are compared with same amount of iodine at same CT scan parameters. The results of in vitro CT scan study show that GNFs have good contrast enhancement properties, whereas in vivo study of rabbits CT scan shows that GNFs enhance the CT image clearly at 135 kV as compared to that of iodine. Cytotoxicity was studied and blood profile show minor increase of white blood cells and haemoglobin, whereas decrease of red blood cells and platelets.

  3. Comparative analysis of whole mount processing and systematic sampling of radical prostatectomy specimens: pathological outcomes and risk of biochemical recurrence.

    Science.gov (United States)

    Salem, Shady; Chang, Sam S; Clark, Peter E; Davis, Rodney; Herrell, S Duke; Kordan, Yakup; Wills, Marcia L; Shappell, Scott B; Baumgartner, Roxelyn; Phillips, Sharon; Smith, Joseph A; Cookson, Michael S; Barocas, Daniel A

    2010-10-01

    Whole mount processing is more resource intensive than routine systematic sampling of radical retropubic prostatectomy specimens. We compared whole mount and systematic sampling for detecting pathological outcomes, and compared the prognostic value of pathological findings across pathological methods. We included men (608 whole mount and 525 systematic sampling samples) with no prior treatment who underwent radical retropubic prostatectomy at Vanderbilt University Medical Center between January 2000 and June 2008. We used univariate and multivariate analysis to compare the pathological outcome detection rate between pathological methods. Kaplan-Meier curves and the log rank test were used to compare the prognostic value of pathological findings across pathological methods. There were no significant differences between the whole mount and the systematic sampling groups in detecting extraprostatic extension (25% vs 30%), positive surgical margins (31% vs 31%), pathological Gleason score less than 7 (49% vs 43%), 7 (39% vs 43%) or greater than 7 (12% vs 13%), seminal vesicle invasion (8% vs 10%) or lymph node involvement (3% vs 5%). Tumor volume was higher in the systematic sampling group and whole mount detected more multiple surgical margins (each p systematic sampling yield similar pathological information. Each method stratifies patients into comparable risk groups for biochemical recurrence. Thus, while whole mount is more resource intensive, it does not appear to result in improved detection of clinically important pathological outcomes or prognostication. Copyright © 2010 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  4. BIOCHEMICAL PARAMETERS OF LIPID METABOLISM IN ANIMALS AFFECTED BY HEAVY METAL SALTS AND TREATED WITH CARNITINE CHLORIDE AND SODIUM ALGINATE

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    I. R. Bekus

    2017-02-01

    Full Text Available Background. Lipid metabolism disorders in the organism affected by environmental pollutants, including poisoning with cadmium and lead salts are of topical matter nowadays. Objective. The study was aimed to examine biochemical features of lipid metabolism in rats subjected to toxic damage by lead and cadmium salts and treated with carnitine chloride and Algigel. Methods. Experiments were carried out on white mature outbred male rats weighing 180-200 g. To cause the toxic damage the animals were administered with aqueous solution of cadmium chloride and lead acetate daily for the period of 30 days using intra-gastric lavage. The indices of lipid metabolism were detected by biochemical methods. Results. In animals treated with cadmium chloride and lead acetate the following changes were observed: HDL-cholesterol concentrations significantly decreased, resulting in 87% of the levels in the intact animals on the third day, 84% on the fifth and 80% on the seventh day. Conversely, concentrations of HDL-cholesterol and VLDL-cholesterol significantly increased during the experiment. Respectively, the ratios for HDL-cholesterol are 240%, 352%, and 388%; and for VLDL-cholesterol 108%, 116%, and 132%. Conclusions. Lipids profile of the rats displayed changes in the levels of cholesterol, triglycerides and lipoproteins of low, high and very low density.

  5. Effect of Salicylic acid on some Growth and Biochemical Parameters of Wheat and Maize Plants under Salt Stress in Vitro

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    Z. Dashagha

    2014-04-01

    Full Text Available In this study, the difference between the resistance of wheat plants (c3 and maize (c4 the salinity was investigated. Research on environmental stresses (Hakimi, 2008 show thatstresses are considered as Limiting factors in crop production.and some phenolic compounds such as salicylic acid are used to improve or alleviate the negative effects of stress. In this study, plants were grown in plastic pots and the plants treated with salicylic acid, after two weeks and seven days later salinity was exerted.The effect of salinity treatmenton both plants, for some morphological and biochemical characteristics were studied. In biochemical tests, lipid peroxidation under salinity and salicylic acid treatments has increased for weat which represents the effect of salinity on the plant and the activetion of the defense mechanism, Howweverthese factors have reduced formaize. Moreover, the increase in total chlorophyll and flavonoids in wheatchlorophyll in wheat and maize shows the role of these pigments in quenching hydrogen peroxide and other active Oxygen types. This increases has not been concideralle in maize. The effect of treatment on the weight of … and root of both plants differed under the investigated concentration.

  6. Cerebral biochemical pathways in experimental autoimmune encephalomyelitis and adjuvant arthritis: a comparative metabolomic study.

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    Norbert W Lutz

    Full Text Available Many diseases, including brain disorders, are associated with perturbations of tissue metabolism. However, an often overlooked issue is the impact that inflammations outside the brain may have on brain metabolism. Our main goal was to study similarities and differences between brain metabolite profiles of animals suffering from experimental autoimmune encephalomyelitis (EAE and adjuvant arthritis (AA in Lewis rat models. Our principal objective was the determination of molecular protagonists involved in the metabolism underlying these diseases. EAE was induced by intraplantar injection of complete Freund's adjuvant (CFA and spinal-cord homogenate (SC-H, whereas AA was induced by CFA only. Naive rats served as controls (n = 9 for each group. Two weeks after inoculation, animals were sacrificed, and brains were removed and processed for metabolomic analysis by NMR spectroscopy or for immunohistochemistry. Interestingly, both inflammatory diseases caused similar, though not identical, changes in metabolites involved in regulation of brain cell size and membrane production: among the osmolytes, taurine and the neuronal marker, N-acetylaspartate, were decreased, and the astrocyte marker, myo-inositol, slightly increased in both inoculated groups compared with controls. Also ethanolamine-containing phospholipids, sources of inflammatory agents, and several glycolytic metabolites were increased in both inoculated groups. By contrast, the amino acids, aspartate and isoleucine, were less concentrated in CFA/SC-H and control vs. CFA rats. Our results suggest that inflammatory brain metabolite profiles may indicate the existence of either cerebral (EAE or extra-cerebral (AA inflammation. These inflammatory processes may act through distinct pathways that converge toward similar brain metabolic profiles. Our findings open new avenues for future studies aimed at demonstrating whether brain metabolic effects provoked by AA are pain/stress-mediated and

  7. Cerebral biochemical pathways in experimental autoimmune encephalomyelitis and adjuvant arthritis: a comparative metabolomic study.

    Science.gov (United States)

    Lutz, Norbert W; Fernandez, Carla; Pellissier, Jean-François; Cozzone, Patrick J; Béraud, Evelyne

    2013-01-01

    Many diseases, including brain disorders, are associated with perturbations of tissue metabolism. However, an often overlooked issue is the impact that inflammations outside the brain may have on brain metabolism. Our main goal was to study similarities and differences between brain metabolite profiles of animals suffering from experimental autoimmune encephalomyelitis (EAE) and adjuvant arthritis (AA) in Lewis rat models. Our principal objective was the determination of molecular protagonists involved in the metabolism underlying these diseases. EAE was induced by intraplantar injection of complete Freund's adjuvant (CFA) and spinal-cord homogenate (SC-H), whereas AA was induced by CFA only. Naive rats served as controls (n = 9 for each group). Two weeks after inoculation, animals were sacrificed, and brains were removed and processed for metabolomic analysis by NMR spectroscopy or for immunohistochemistry. Interestingly, both inflammatory diseases caused similar, though not identical, changes in metabolites involved in regulation of brain cell size and membrane production: among the osmolytes, taurine and the neuronal marker, N-acetylaspartate, were decreased, and the astrocyte marker, myo-inositol, slightly increased in both inoculated groups compared with controls. Also ethanolamine-containing phospholipids, sources of inflammatory agents, and several glycolytic metabolites were increased in both inoculated groups. By contrast, the amino acids, aspartate and isoleucine, were less concentrated in CFA/SC-H and control vs. CFA rats. Our results suggest that inflammatory brain metabolite profiles may indicate the existence of either cerebral (EAE) or extra-cerebral (AA) inflammation. These inflammatory processes may act through distinct pathways that converge toward similar brain metabolic profiles. Our findings open new avenues for future studies aimed at demonstrating whether brain metabolic effects provoked by AA are pain/stress-mediated and/or due to the

  8. Comparative Biochemical and Functional Analysis of Viral and Human Secreted Tumor Necrosis Factor (TNF) Decoy Receptors*

    Science.gov (United States)

    Pontejo, Sergio M.; Alejo, Ali; Alcami, Antonio

    2015-01-01

    The blockade of tumor necrosis factor (TNF) by etanercept, a soluble version of the human TNF receptor 2 (hTNFR2), is a well established strategy to inhibit adverse TNF-mediated inflammatory responses in the clinic. A similar strategy is employed by poxviruses, encoding four viral TNF decoy receptor homologues (vTNFRs) named cytokine response modifier B (CrmB), CrmC, CrmD, and CrmE. These vTNFRs are differentially expressed by poxviral species, suggesting distinct immunomodulatory properties. Whereas the human variola virus and mouse ectromelia virus encode one vTNFR, the broad host range cowpox virus encodes all vTNFRs. We report the first comprehensive study of the functional and binding properties of these four vTNFRs, providing an explanation for their expression profile among different poxviruses. In addition, the vTNFRs activities were compared with the hTNFR2 used in the clinic. Interestingly, CrmB from variola virus, the causative agent of smallpox, is the most potent TNFR of those tested here including hTNFR2. Furthermore, we demonstrate a new immunomodulatory activity of vTNFRs, showing that CrmB and CrmD also inhibit the activity of lymphotoxin β. Similarly, we report for the first time that the hTNFR2 blocks the biological activity of lymphotoxin β. The characterization of vTNFRs optimized during virus-host evolution to modulate the host immune response provides relevant information about their potential role in pathogenesis and may be used to improve anti-inflammatory therapies based on soluble decoy TNFRs. PMID:25940088

  9. Comparative Biochemical and Functional Analysis of Viral and Human Secreted Tumor Necrosis Factor (TNF) Decoy Receptors.

    Science.gov (United States)

    Pontejo, Sergio M; Alejo, Ali; Alcami, Antonio

    2015-06-26

    The blockade of tumor necrosis factor (TNF) by etanercept, a soluble version of the human TNF receptor 2 (hTNFR2), is a well established strategy to inhibit adverse TNF-mediated inflammatory responses in the clinic. A similar strategy is employed by poxviruses, encoding four viral TNF decoy receptor homologues (vTNFRs) named cytokine response modifier B (CrmB), CrmC, CrmD, and CrmE. These vTNFRs are differentially expressed by poxviral species, suggesting distinct immunomodulatory properties. Whereas the human variola virus and mouse ectromelia virus encode one vTNFR, the broad host range cowpox virus encodes all vTNFRs. We report the first comprehensive study of the functional and binding properties of these four vTNFRs, providing an explanation for their expression profile among different poxviruses. In addition, the vTNFRs activities were compared with the hTNFR2 used in the clinic. Interestingly, CrmB from variola virus, the causative agent of smallpox, is the most potent TNFR of those tested here including hTNFR2. Furthermore, we demonstrate a new immunomodulatory activity of vTNFRs, showing that CrmB and CrmD also inhibit the activity of lymphotoxin β. Similarly, we report for the first time that the hTNFR2 blocks the biological activity of lymphotoxin β. The characterization of vTNFRs optimized during virus-host evolution to modulate the host immune response provides relevant information about their potential role in pathogenesis and may be used to improve anti-inflammatory therapies based on soluble decoy TNFRs. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. Biochemical liver function test parameter levels in relation to treatment response in liver metastatic colorectal patients treated with FOLFOX4 with or without bevacizumab

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    Denić Kristina

    2016-01-01

    Full Text Available Introduction. Combined use of bevacizumab and conventional anticancer drugs leads to a significant improvement of treatment response in patients with metastatic colorectal carcinoma (CRC. Conventional treatment protocols exert undesired effects on the liver tissue. Hepatotoxic effects are manifested as a disturbance of liver function test parameters. The relation between clinical outcome and disorder of biochemical parameters has not been completely evaluated. Objective. The objective of our study was to examine whether clinical outcome in patients with liver metastatic CRC correlates with the level of liver function test parameters. Methods. The study included 96 patients with untreated liver metastatic CRC who received FOLFOX4 protocol with or without bevacizumab. Biochemical liver parameters were performed before and after the treatment completion. Treatment response was evaluated as disease regression, stable disease, and disease progression. The patients were divided into three groups according to the accomplished treatment response. Results. In the group of patients with disease regression the post-treatment levels of aspartate aminotransferase, alanine aminotransferase, and bilirubin were statistically significantly increased. In contrast to this, gamma-glutamyltransferase and protein post-treatment values were significantly lower in relation to initial values. In patients with stable disease, difference was found only in the level of proteins being lower after the treatment. In patients with disease progression, values of aspartate aminotransferase and bilirubin were significantly increased after completed treatment. Conclusion. Treatment responses are not completely associated with the level of liver function test parameters. The only parameter which correlated with treatment response is gamma-glutamyltransferase. Its decrease is accompanied with disease regression.

  11. Prognostic value of metabolic parameters and clinical impact of {sup 18}F-fluorocholine PET/CT in biochemical recurrent prostate cancer

    Energy Technology Data Exchange (ETDEWEB)

    Colombie, M.; Bailly, C.; Rusu, D.; Rousseau, N. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Campion, L. [ICO Cancer Center, Statistics, Saint-Herblain (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, T. [Urologic Clinic Nantes-Atlantis, Saint Herblain (France); Mathieu, C. [University Hospital, Nuclear Medicine, Nantes (France); Ferrer, L. [ICO Cancer Center, Physics, Saint-Herblain (France); Kraeber-Bodere, F. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); University Hospital, Nuclear Medicine, Nantes (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France); Rousseau, C. [Institut de Cancerologie de l' Ouest Rene Gauducheau, Nuclear Medicine, 44805 Nantes-St Herblain Cedex (France); Nantes University, Nantes-Angers Cancer Research Center, INSERM U892-CNRS UMR 6299, Nantes (France)

    2015-11-15

    To evaluate the therapeutic impact of {sup 18}F-fluorocholine (FCH) PET/CT in biochemical recurrent prostate cancer (PC) and to investigate the value of quantitative FCH PET/CT parameters in predicting progression-free survival (PFS). This retrospective study included 172 consecutive patients with PC who underwent FCH PET/CT for biochemical recurrence. Mean rising PSA was 10.7 ± 35.0 ng/ml. Patients with positive FCH PET were classified into three groups: those with uptake only in the prostatic bed, those with locoregional disease, and those with distant metastases. Referring physicians were asked to indicate the hypothetical therapeutic strategy with and without the FCH PET/CT results. Clinical variables and PET parameters including SUVmax, SUVpeak, SUVmean, total lesion choline kinase activity (TLCKA) and standardized added metabolic activity (SAM) were recorded and a multivariate analysis was performed to determine the factors independently predicting PFS. In 137 of the 172 patients, the FCH PET/CT scan was positive, and of these, 29.9 % (41/137) had prostatic recurrence, 42.3 % (58/137) had pelvic lymph node recurrence with or without prostatic recurrence, and 27.7 % (38/137) had distant metastases. The FCH PET/CT result led to a change in treatment plan in 43.6 % (75/172) of the 172 patients. Treatment was changed in 49.6 % (68/137) of those with a positive FCH PET/CT scan and in 20 % (7/35) of those with a negative FCH PET/CT scan. After a median follow-up of 29.3 months (95 % CI 18.9 - 45.9 months), according to multivariate analysis age <70 years, SAM ≥23 and SUVmean ≥3 were parameters independently predicting PFS. A nomogram constructed using the three parameters showed 49 months of PFS in patients with the best scores (0 or 1) and only 11 months in patients with a poor score (score 3). This study indicates that a positive FCH PET result in PC patients with biochemical recurrence predicts a shorter PFS and confirms the major impact of the FCH PET

  12. Ameliorative effect of hydro-methanolic extract from roots of Rauwolfia serpentina on some biochemical parameters of type 1 diabetic mice.

    Science.gov (United States)

    Azmi, Muhammad Bilal; Qureshi, Shamim A; Ahmed, Syed Danish Haseen; Mudassir, Hina Akram; Imtiaz, Fauzia; Rais, Sumera; Khan, Auwais Ahmed; Ahsan, Muhammad

    2018-03-01

    Present work seeks to investigate the biochemical parameters in terms of hypoglycemic and hypolipidemic effects of hydro-methanolic roots extract (HyMREt) of Rauwolfia serpentina in type 1 (alloxan induced) diabetic mice. Animals were divided into seven groups, four control groups, and three were test groups (HyMREt at 50, 100, & 150mg/kg). Each treatment was repeated for 14 days regularly in all seven respective groups and afterwards the body weights, fasting blood glucose (FBG), insulin, and serum lipid levels were determined. Total body weights of diabetic mice treated with HyMREt extract were dose dependently (pserpentina.

  13. A case-control analysis of common variants in GIP with type 2 diabetes and related biochemical parameters in a South Indian population

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    Kumar Harish

    2010-07-01

    Full Text Available Abstract Background Glucose-dependent insulinotropic polypeptide (GIP is one of the incretins, which plays a crucial role in the secretion of insulin upon food stimulus and in the regulation of postprandial glucose level. It also exerts an effect on the synthesis and secretion of lipoprotein lipase, from adipocytes, important for lipid metabolism. The aim of our study was to do a case-control association analysis of common variants in GIP in association with type 2 diabetes and related biochemical parameters. Method A total of 2000 subjects which includes 1000 (584M/416F cases with type 2 diabetes and 1000 (470M/530F normoglycemic control subjects belonging to Dravidian ethnicity from South India were recruited to assess the effect of single nucleotide polymorphisms (SNPs in GIP (rs2291725, rs2291726, rs937301 on type 2 diabetes in a case-control manner. The SNPs were genotyped by using tetra primer amplification refractory mutation system-PCR (ARMS PCR. For statistical analysis, our study population was divided into sub-groups based on gender (male and female. Association analysis was carried out using chi-squared test and the comparison of biochemical parameters among the three genotypes were performed using analysis of covariance (ANCOVA. Result Initial analysis revealed that, out of the total three SNPs selected for the present study, two SNPs namely rs2291726 and rs937301 were in complete linkage disequilibrium (LD with each other. Therefore, only two SNPs, rs2291725 and rs2291726, were genotyped for the association studies. No significant difference in the allele frequency and genotype distribution of any of the SNPs in GIP were observed between cases and controls (P > 0.05. Analysis of biochemical parameters among the three genotypes showed a significant association of total cholesterol (P = 0.042 and low density lipoprotein (LDL with the G allele of the SNP rs2291726 in GIP (P = 0.004, but this was observed only in the case of female

  14. Evaluation of turmeric (Curcuma longa) effect on biochemical and pathological parameters of liver and kidney in chicken aflatoxicosis.

    Science.gov (United States)

    Gholami-Ahangaran, Majid; Rangsaz, Nader; Azizi, Shahrzad

    2016-01-01

    Aflatoxins as potent mycotoxins can influence vital parameters in chickens. Turmeric was used in decreasing toxic effect of mycotoxins on vital organs, traditionally. The study compared the protective effect of turmeric and Mycoad(TR) in broilers exposed to aflatoxin. Chickens (270) were divided into six groups. The chickens were fed a basal diet, turmeric extract (5 mg/kg diet), Mycoad(TR) (25 mg/kg diet), productive aflatoxin (3 mg/kg diet), aflatoxin plus turmeric extract (3 versus 5 mg/kg diet), and aflatoxin plus Mycoad(TR) (3 versus 25 mg/kg diet) in basal diet. At 28 d old, we determined plasma concentration of total protein, albumin, triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), calcium, potassium, phosphorous, uric acid, aspartate transferase (AST), and alanine aminotransferase (ALT). Furthermore, liver and kidney were sampled for pathological examination. Chickens fed turmeric with aflatoxin had significant lower ALT, AST, and uric acid than chickens fed aflatoxin (11.4 ± 0.79, 228 ± 9, and 6 ± 0.4 versus 17.2 ± 1.7, 283 ± 5, and 7.7 ± 0.1) whereas, total protein, calcium, and HDL values in chickens fed aflatoxin plus turmeric increased significantly (2.66 ± 0.16, 8.4 ± 0.2, and 920 ± 4.1 versus 1.7 ± 0.17, 7 ± 0.2, and 690 ± 4.8). Pathological examination revealed severe congestion, degeneration, and necrosis in liver and kidney in chickens that received aflatoxin. The study showed that turmeric may provide protection against the toxic effects of aflatoxin on liver and kidney.

  15. Repeatability of quantitative parameters of 18F-fluoride PET/CT and biochemical tumour and specific bone remodelling markers in prostate cancer bone metastases.

    Science.gov (United States)

    Wassberg, Cecilia; Lubberink, Mark; Sörensen, Jens; Johansson, Silvia

    2017-12-01

    18F-fluoride PET/CT exhibits high sensitivity to delineate and measure the extent of bone metastatic disease in patients with prostate cancer. 18F-fluoride PET/CT could potentially replace traditional bone scintigraphy in clinical routine and trials. However, more studies are needed to assess repeatability and biological uptake variation. The aim of this study was to perform test-retest analysis of quantitative PET-derived parameters and blood/serum bone turnover markers at the same time point. Ten patients with prostate cancer and verified bone metastases were prospectively included. All underwent two serial 18F-fluoride PET/CT at 1 h post-injection. Up to five dominant index lesions and whole-body 18F-fluoride skeletal tumour burden were recorded per patient. Lesion-based PET parameters were SUVmax, SUVmean and functional tumour volume applying a VOI with 50% threshold (FTV 50% ). The total skeletal tumour burden, total lesion 18F-fluoride (TLF), was calculated using a threshold of SUV of ≥15. Blood/serum biochemical bone turnover markers obtained at the time of each PET were PSA, ALP, S-osteocalcin, S-beta-CTx, 1CTP and BAP. A total of 47 index lesions and a range of 2-122 bone metastases per patient were evaluated. Median time between 18F-fluoride PET/CT was 7 days (range 6-8 days). Repeatability coefficients were for SUVmax 26%, SUVmean 24%, FTV 50% for index lesions 23% and total skeletal tumour burden (TLF) 35%. Biochemical bone marker repeatability coefficients were for PSA 19%, ALP 23%, S-osteocalcin 18%, S-beta-CTx 22%, 1CTP 18% and BAP 23%. Quantitative 18F-fluoride uptake and simultaneous biochemical bone markers measurements are reproducible for prostate cancer metastases and show similar magnitude in test-retest variation.

  16. Seasonal and gender-related differences in morphometric features and cellular and biochemical parameters of Carcinus aestuarii from the Lagoon of Venice.

    Science.gov (United States)

    Matozzo, Valerio; Boscolo, Alice; Marin, Maria Gabriella

    2013-08-01

    In this study, the seasonal variations in the morphometric features and in the cellular and biochemical parameters of the haemolymph were investigated in both male and female crabs (Carcinus aestuarii). Crabs were seasonally (November 2010-August 2011) collected from the Lagoon of Venice, and the moult stage, weight, width and length of the carapace, and width and length of the bigger chela were evaluated. In addition, the total haemocyte count (THC), haemocyte diameter and volume, haemolymph glucose and total protein levels, and haemolymph phenoloxidase (PO) and N-acetyl-β-glucosaminidase (NAG) activities were measured. The results demonstrated that the collected crabs were all in the intermoult stage and that the males were bigger than the females. A two-way ANOVA revealed a significant effect of season on the THC and the haemocyte volume and a significant influence of gender on the haemocyte diameter. Season and gender significantly affected the haemolymph glucose concentration, whereas haemolymph protein levels were dependent only on the season. In addition, both season and gender significantly influenced the PO and NAG activities in the haemolymph. Overall, the results demonstrated that crab morphometric features as well as haemolymph cellular and biochemical parameters varied markedly as a function of both season and gender. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Relationships between certain metabolic diseases and selected serum biochemical parameters in seropositive dairy cows against Neospora caninum infection in different stages of lactation

    Science.gov (United States)

    Alekish, Myassar O.; Talafha, Abdelsalam Q; Alshehabat, Musa A; Ismail, Zuhair A Bani

    Neospora caninum is an important cause of abortion in dairy cattle. The general health of affected cows has not been investigated before. Therefore, the main objective of this study was to identify possible relationships between certain metabolic diseases and selected serum biochemical parameters in seropositive dairy cows against N. caninum antibodies in different stages of lactation. The study was carried out using 72 N. caninum seropositive cows and 61 seronegative dairy cows (control). Serum from all cows was tested to determine their N. caninum status (seropositive vs seronegative) using commercially available indirect enzyme-linked immunosorbent assay test kit (iELISA). In addition, serum biochemical parameters including beta-hydroxybutyrate (BHB), glucose, creatinine, blood urea nitrogen, total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) and gamma-glutamyltranspeptidase (GGT) were determined using routine laboratory methods. The stage of lactation was obtained at the time of sampling from farm records. Student independent t-test showed that there was a significant difference in the serum concentrations of BHB, AST, ALT, and LDH between seropositive and seronegative cows. There was no significant association between seropositivity and the stage of lactation. However, multivariable logistic regression analysis showed that there was a strong association between seropositivity and BHB concentrations. Results of this study indicate a possible relationship between N. caninum seropositivity and certain metabolic diseases such as ketosis and fatty liver syndrome in dairy cows.

  18. Effect of different levels of selenium and vitamin E on blood biochemical parameters in the Japanese quail

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    hossein zadeh adamnejad

    2015-11-01

    Full Text Available Co-administration of vitamin E and selenium has positive effects on growth performance of avian species, but their effect is not fully elicited in the Japanese quail. Also, controversial reports exist for the effect of vitamin E and selenium on avian performance. This study was designed to investigate the effect of different levels of selenium (0, 0.2 and 0.4 mg/kg and vitamin E (0, 150 and 300 mg/kg in the diet of the Japanese quail. Birds received mesh diet from the start of the study, then selenium and vitamin E were added on day 17. At the end of the study, after food deprivation for 6 hours, Japanese quail (1 male and 1 female were selected and blood samples collected for biochemical investigations and determination of glucose, albumin, total protein, cholesterol, triglyceride, HDL and LDL levels. There was significant decrease in cholesterol, glucose, and triglyceride levels and significant decrease in albumin and total protein levels on day 35 (p

  19. Effect of Short Term NaCl Stress on Cultivars of S. lycopersicum: A Comparative Biochemical Approach

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    Chaitali Roy

    2014-03-01

    Full Text Available Tomato is a crop plant with high fruit nutritive value and other useful properties. The cultivation of this species is dependent on many environmental factors, e.g. temperature, salinity, nutrients etc, affecting the yield and reproductive potential of the plant. Salinity in soil or water is of increasing importance to agriculture because it causes stress to crop plants. Plants exposed to an excess amount of salts such as NaCl undergo osmotic stress, water deficit and ionic imbalances and can increase production of reactive oxygen species(ROS. Higher plants possess very efficient enzymatic and non-enzymatic antioxidative defense mechanisms that allow the scavenging of ROS and protection of cellular components from oxidative damage. Studies were conducted to investigate the effect of short term salinity stress on some physiological alterations in three tomato cultivars Pusa Ruby(PR, Punjab Keshari (PK and Ailsa Craig(AC. Some biochemical parameters (anthocyanin and carotenoeid content, polyamines, proline, cysteine, peroxidase and malondialdehyde were set and applied at two month old stage of tomato plants. Three tomato cultivars were grown in 0.5xMS for 2 months and at this stage, they were treated with 0 and 200mM NaCl for a short period of six hours in hydroponic conditions. The genotypes exhibited different responses in terms of different osmoprotectant, antioxidant, and pigment level. The relationships among the salinity and accumulation of these compounds in leaf were then determined. It was concluded that, tomato cultivars under study responded differently showing their sensitivity or tolerance to salinity stress. Among three cultivars PK appeared to be more tolerant genotype than the other two cultivars PR and AC. PK could rapidly evolve physiological and antioxidant mechanisms to adapt to salt and manage the oxidative stress. The research was conducted in a completely randomized design with three replications.

  20. STUDY OF CORRELATION AND SIGNIFICANCE OF BIOCHEMICAL PARAMETERS IN THE ASSESSMENT OF ACTIVE PHASE OF THYROID EYE DISEASE

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    Ashok Kumar P

    2016-08-01

    Full Text Available AIM Role of Interleukin-6 and HS-CRP Levels in the assessment of active thyroid eye disease. MATERIALS & METHODS A prospective observational study of 30 patients from the age group of 20-60 years with thyroid eye disease done at Regional Institute of Ophthalmology. All patients were subjected to detailed history, ocular examination, systemic examination, biochemical analysis and Imaging. Patients are categorised as mild, moderate and severe active disease based on clinical activity score. For the period of two years, patients are followed at regular periodic intervals according to the severity of disease and results were analysed. RESULTS Out of 30 patients studied, majority of the patients were females (60%, and 70% of the patients had bilateral disease. Among 30 patients, 83.33% in hyperthyroid state, 3.33% in Hypothyroid state, 13.33% in Euthyroid state (Table 3. Smoking being important risk factor in 30% among males. 50% of patients presented with mild disease, 30% with moderate disease, 0% with severe disease. IL-6, HS-CRP levels are increased only in patients with severe active disease (Table 6. Remission attained in all patients when treated earlier with steroids. CONCLUSION Identifying disease activity early and aggressive treatment with systemic steroids in active phase of moderate and severe disease has reduced the morbidity associated with disease. Correlation of IL-6, HS-CRP, TFT levels are significantly increased only in patients with active phase of severe thyroid eye disease but not significantly elevated in active phase of moderate disease. Another pitfall is IL-6 is an expensive ELISA based diagnosis. Thus, IL-6 & HS-CRP cannot be routinely used to screen patients with Thyroid eye disease.

  1. [The use of controlled physical training in patients with acute coronary syndrome treated with intervention - assessment of effects on biochemical parameters and functional myocardial].

    Science.gov (United States)

    Kapusta, Joanna; Kapusta, Anna; Pawlicki, Lucjan; Irzmański, Robert

    2016-06-01

    Diseases of the cardiovascular system is one of the most common causes of death among people over 65 years. Due to its course and incidence are a major cause of disability and impaired quality of life for seniors, as well as a serious economic problem in health care. Important role in the prevention of cardiovascular disease plays making systematic physical activity, which is a component of any rehabilitation program. Regular physical training by doing cardio-and vasoprotective has a beneficial effect on cardiovascular status and physical performance in patients with diagnosed coronary heart disease, regardless of age. The aim of this study was to evaluate the effect of controlled exercise on selected biochemical parameters and functional myocardial infarction. A group of 89 patients were divided into 3 subgroups. In group I (n = 30) was performed 2 weeks cardiac rehabilitation program, in group II (n = 30) 4 weekly. Streamline the program consisted of a series of interval training performed using a bicycle ergometer and general exercise. The remaining group (gr. III, n = 29) participated in individually selected training program. In all subjects before and after the training cycle underwent thoracic impedance plethysmography, also determined the level of plasma natriuretic peptide NT-proBNP and echocardiography and exercise test. After training, in groups, which carried out a controlled physical training, improvement was observed: exercise capacity of patients respectively in group I (p = 0.0003), group II (p = 0.0001) and group III (p = 0.032), stroke volume SV, cardiac output CO and global myocardial contractility, there was also reduction in the concentration of natriuretic peptide NT-proBNP. Furthermore, the correlation between the results shown pletyzmography parameters and NT-proBNP, SV, CO and EF. Regular physical training as part of the cardiac rehabilitation has a beneficial effect on biochemical parameters and functional myocardial infarction in patients

  2. Effect of a trans fatty acid-enriched diet on biochemical and inflammatory parameters in Wistar rats.

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    Longhi, Rafael; Almeida, Roberto Farina; Machado, Letiane; Duarte, Maria Marta Medeiros Frescura; Souza, Débora Guerini; Machado, Priscila; de Assis, Adriano Martimbianco; Quincozes-Santos, André; Souza, Diogo Onofre

    2017-04-01

    Recent data regarding trans fatty acids (TFAs) have implicated these lipids as particularly deleterious to human health, causing systemic inflammation, endothelial dysfunction and possibly inflammation in the central nervous system (CNS). We aimed to clarify the impact of partially hydrogenated soybean oil (PHSO) with different TFA concentrations on cerebrospinal fluid (CSF), serum and hepatic parameters in adult Wistar rats. Wistar rats (n = 15/group) were fed either a normolipidic diet or a hyperlipidic diet for 90 days. The normolipidic and hyperlipidic diets had the same ingredients except for fat compositions, concentrations and calories. We used lard in the cis fatty acid group and PHSO in the trans fatty acid group. The intervention groups were as follows: (1) low lard (LL), (2) high lard (HL), (3) low partially hydrogenated soybean oil (LPHSO) and (4) high partially hydrogenated soybean oil (HPHSO). Body weight, lipid profiles and the inflammatory responses in the CSF, serum and liver tissue were analyzed. Surprisingly, with the PHSO diet we observed a worse metabolic response that was associated with oxidative stress in hepatic tissue as well as impaired serum and CSF fluid parameters at both PHSO concentrations. In many analyses, there were no significant differences between the LPHSO and HPHSO diets. Dietary supplementation with PHSO impaired inflammatory parameters in CSF and blood, induced insulin resistance, altered lipid profiles and caused hepatic damage. Overall, these findings suggest that fat composition is more important than the quantity of fat consumed in terms of cis and trans fatty acid diets.

  3. Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and Leporinus obtusidens (Valenciennes, 1837

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    Vania Lucia Loro

    Full Text Available Rhamdia quelen (silver catfish and Leporinus obtusidens (piava were exposed to a commercial formulation Roundup(r, a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT and aspartate aminotransferase (AST activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase activities. Plasmatic glucose levels were reduced in both species, whereas the transaminase activities (ALT and AST increased after exposure to the herbicide. Herbicide exposure increased protein and glucose levels in the mucus layer in both species. There was a reduction in both NTPDase and ecto-5'-nucleotidase activity in the brain of piava, and increased enzyme activity in silver catfish at both concentrations tested. The species showed an increase in protein carbonyl in the liver after exposure to both concentrations of the glyphosate. Our results demonstrated that exposure to Roundup(r caused liver damage, as evidenced by increased plasma transaminases and liver protein carbonyl in both of the fish species studied. The mucus composition changed and hypoglycemia was detected after Roundup(r exposure in both species. Brain nucleotide hydrolysis showed a different response for each fish species studied. These parameters indicated some important and potential indicators of glyphosate contamination in aquatic ecosystems.

  4. Effects of different rearing systems on growth performance, carcass traits, meat quality and serum biochemical parameters of Chaohu ducks.

    Science.gov (United States)

    Zhang, Cheng; Ah Kan Razafindrabe, Richard-Hermann; Chen, Kaikai; Zhao, Xiaohui; Yang, Lei; Wang, Li; Chen, Xingyong; Jin, Sihua; Geng, Zhaoyu

    2018-04-01

    This study was conducted using a total of 360 22-day-old Chaohu ducks to evaluate the effect of rearing system on growth performance, carcass traits, meat quality and serum parameters of male and female Chaohu ducks. The birds were divided and raised in separate pens according to sex and rearing system, with three replicate pens of 30 male or 30 female ducks per pen for each rearing system. The rearing systems consisted of a floor rearing system (FRS) and a net rearing system (NRS). Results showed that ducks raised in NRS had better growth performance, whereas, ducks raised in FRS exhibited better carcass traits and meat color, and lower intramuscular fat. For the serum parameters, NRS significantly decreased high-density lipoprotein cholesterol content, and enhanced total protein and triacylglycerol contents. Male ducks had lower abdominal fat percentage, and higher growth performance and shear force, but there were no other significant differences between sexes. No rearing system × sex interaction was observed in the present study, revealing that rearing system had the same effect on both sexes. In conclusion, NRS was beneficial to the growth performance of Chaohu ducks, whereas this system had some negative effects on carcass traits, meat quality and serum profiles. © 2018 Japanese Society of Animal Science.

  5. Changes in biochemical and microbiological parameters during the period of rapid composting of dairy manure with rice chaff.

    Science.gov (United States)

    Liu, Dongyang; Zhang, Ruifu; Wu, Hongsheng; Xu, Dabing; Tang, Zhu; Yu, Guanghui; Xu, Zhihui; Shen, Qirong

    2011-10-01

    Various parameters were measured during the period of composting of dairy manure and rice chaff in different ratios (dairy manure/rice chaff=V/V, pile 1: 75/25; pile 2: 80/20; pile 3: 85/15) to evaluate their suitability as indicators for the composting process. The temperature in pile 1 increased rapidly and remained above 60 °C for 30 days, while the temperature in pile 3 increased slowly relative to the other two piles. Furthermore, the degradation of organic substrates, as indicated by the reduction of C/N ratio, was rapid in pile 1 (below 20% 28 days after beginning of the composting). The major fluctuations of various water-soluble fractions in all piles were observed during the first 3 weeks, and the results in general showed that the highest microbial populations and enzymatic activities also appeared in this phase. Various parameters indicated that the rapid composting method was a feasible one for treating agricultural wastes. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Evaluation of different agroindustrial waste on the effect of different carcass characteristics and physiological and biochemical parameters in broilers chicken

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    Y. Sanchez-Roque

    2017-04-01

    Full Text Available Aim: This study was conducted to evaluate the physiological and biochemical effect in chickens of the Ross breed of a food enriched with agroindustrial waste. Materials and Methods: The food is one of the main components of the total cost for the production of chickens. Rations should be formulated to provide the correct balance of energy, protein, amino acids, minerals, vitamins and essential fatty acids, to allow optimal growth and performance. This study was intended to evaluate a natural feed for chicken, made from corn, yucca meal, eggshells, orange peel, soybean meal, salt and garlic, enriched with agroindustrial waste (molasses, milk whey and ferment of coffee. The weight gain was evaluated in broilers using a diet enriched with different agroindustrial wastes, with respect to a control food of the same composition but not containing residue. To develop the experiment 120 male Ross 308 chicks were used, these were evaluated for 6 weeks. Physicochemical test for the food and the agroindustrial waste were performed; moisture was determined; organic carbon, organic material and the ash, to characterize the agroindustrial wastes, the reducing sugars content using a spectrophotometer at 540 nm and proteins through the Kjeldahl method was evaluated. During the experiment, the weight gain of chickens and feed conversion was evaluated; the end of the experiment the weight of eviscerated channel relative weight breast, thighs, pancreas, and abdominal fat was determined, besides including blood chemistries as determination of cholesterol, triglycerides, and glucose. Finally, the microbiological analyzes to detect the presence of Escherichia coli in the cecum was determinate. Data were analyzed by InfoStat statistical program using the generalized linear model procedure. The statistical comparison was made by Tukey test at the 95% probability level. Results: After the evaluation, fed chickens with the treatments food + milk whey (FMW and food

  7. Analysis of the Variability of Epstein-Barr Virus Genes in Infectious Mononucleosis: Investigation of the Potential Correlation with Biochemical Parameters of Hepatic Involvement.

    Science.gov (United States)

    Banko, Ana; Lazarevic, Ivana; Stevanovic, Goran; Cirkovic, Andja; Karalic, Danijela; Cupic, Maja; Banko, Bojan; Milovanovic, Jovica; Jovanovic, Tanja

    2016-09-01

    Primary Epstein-Barr virus (EBV) infection is usually asymptomatic, although at times it results in the benign lymphoproliferative disease, infectious mononucleosis (IM), during which almost half of patients develop hepatitis. The aims of the present study are to evaluate polymorphisms of EBV genes circulating in IM isolates from this geographic region and to investigate the correlation of viral sequence patterns with the available IM biochemical parameters. The study included plasma samples from 128 IM patients. The genes EBNA2, LMP1 , and EBNA1 were amplified using nested-PCR. EBNA2 genotyping was performed by visualization of PCR products using gel electrophoresis. Investigation of LMP1 and EBNA1 included sequence, phylogenetic, and statistical analyses. The presence of EBV DNA in plasma samples showed correlation with patients' necessity for hospitalization (p=0.034). The majority of EBV isolates was genotype 1. LMP1 variability showed 4 known variants, and two new deletions (27-bp and 147-bp). Of the 3 analyzed attributes of LMP1 isolates, the number of 33-bp repeats less than the reference 4.5 was the only one that absolutely correlated with the elevated levels of transaminases. EBNA1 variability was presented by prototype subtypes. A particular combination of EBNA2, LMP1 , and EBNA1 polymorphisms, deleted LMP1/P-thr and non-deleted LMP1/P-ala , as well as genotype 1/ 4.5 33-bp LMP1 repeats or genotype 2/ 4.5 33-bp LMP1 repeats showed correlation with elevated AST (aspartate aminotransferase) and ALT (alanine transaminase). This is the first study which identified the association between EBV variability and biochemical parameters in IM patients. These results showed a possibility for the identification of hepatic related diagnostic EBV markers.

  8. The influence of altitude and landforms on some biochemical and hematological parameters in Ouled Djellal ewes from arid area of South East Algeria

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    Mohammed Titaouine

    2015-01-01

    Full Text Available Aim: This study was conducted on Ouled Djellal ewes in arid area of south-east Algeria in order to reveal the influence of altitude and landforms on some hematological and biochemical parameters. Materials and Methods: A total of 160 ewes having 3-5 years of age, multiparous, non-pregnant, non-lactating and reared in arid areas of South East Algeria were included. Blood samples were divided according to factors of altitude and landform (plain region at 150 m above sea level, tableland region at 600 m above sea level and mountain region at 1000 m above sea level. The whole blood was analyzed for hematology, and plasma samples for biochemical analysis. Results: The study found lowest glucose concentrations were detected in tableland region at 600 m. In plain region at 150 m, ewes had a higher (p<0.01 concentration of cholesterol and triglyceride. Furthermore, a higher concentration of total proteins (p<0.01 and urea (p<0.05 were detected in plain region at 150 m. The average blood creatinine concentration in mountain ewes at 1000 m and tableland ewes at 600 m were higher (p<0.05 that in plain ewes at 150 m. The highest calcium concentration was found at the altitude of 150 m and the lowest at the altitude of 1000 m (1.12±0.35 mmol/L vs. 0.52±0.03 mmol/L. Phosphorus levels were higher at altitudes of 150 m than at the altitude of 600 m and 1000 m (0.93±0.42 mmol/L vs. 0.68±0.54 mmol/L, 0.23±0.01 mmol/L. The highest hemoglobin concentration and value of hematocrit were detected in mountain ewes at the altitude of 1000 m (120.61 g/L, 40% and the lowest at the altitude of 150 m (73.2 g/L, 31% (p<0.001. Conclusion: We concluded that hematological and biochemical parameters in Ouled Djellel ewes reared in arid area may be affected by altitude and landforms.

  9. Diclocor is superior to diclofenac sodium and quercetin in normalizing biochemical parameters in rats with collagen-induced osteoarthritis.

    Science.gov (United States)

    Zupanets, I A; Shebeko, S K; Popov, O S; Shalamay, A S

    2016-02-01

    The aim of the present study was to investigate anti-inflammatory activity of Diclocor in the setting of collagen-induced osteoarthritis in rats in comparison with its active monocomponents-diclofenac sodium and quercetin. The study was conducted on the model of collagen-induced osteoarthritis and included measurement of sialic acids, glycoproteins, C-reactive protein, prostaglandin E2, 6-keto-prostaglandin F1α, thromboxane B2, and leukotriene B4. Lastly, morphologic study with morphometry was also performed. Diclocor is superior to quercetin and diclofenac sodium by the degree of pharmacological effect on some of the studied parameters. The differences between the values were statistically significant. Diclocor is a promising corrector of inflammatory and destructive joint diseases. Owing to the presence of both diclofenac sodium and quercetin in its composition, Diclocor exhibits a complex mechanism of anti-inflammatory action affecting cyclooxygenase and lipoxygenase ways of arachidonic acid biotransformation.

  10. An improved hybrid of particle swarm optimization and the gravitational search algorithm to produce a kinetic parameter estimation of aspartate biochemical pathways.

    Science.gov (United States)

    Ismail, Ahmad Muhaimin; Mohamad, Mohd Saberi; Abdul Majid, Hairudin; Abas, Khairul Hamimah; Deris, Safaai; Zaki, Nazar; Mohd Hashim, Siti Zaiton; Ibrahim, Zuwairie; Remli, Muhammad Akmal

    2017-12-01

    Mathematical modelling is fundamental to understand the dynamic behavior and regulation of the biochemical metabolisms and pathways that are found in biological systems. Pathways are used to describe complex processes that involve many parameters. It is important to have an accurate and complete set of parameters that describe the characteristics of a given model. However, measuring these parameters is typically difficult and even impossible in some cases. Furthermore, the experimental data are often incomplete and also suffer from experimental noise. These shortcomings make it challenging to identify the best-fit parameters that can represent the actual biological processes involved in biological systems. Computational approaches are required to estimate these parameters. The estimation is converted into multimodal optimization problems that require a global optimization algorithm that can avoid local solutions. These local solutions can lead to a bad fit when calibrating with a model. Although the model itself can potentially match a set of experimental data, a high-performance estimation algorithm is required to improve the quality of the solutions. This paper describes an improved hybrid of particle swarm optimization and the gravitational search algorithm (IPSOGSA) to improve the efficiency of a global optimum (the best set of kinetic parameter values) search. The findings suggest that the proposed algorithm is capable of narrowing down the search space by exploiting the feasible solution areas. Hence, the proposed algorithm is able to achieve a near-optimal set of parameters at a fast convergence speed. The proposed algorithm was tested and evaluated based on two aspartate pathways that were obtained from the BioModels Database. The results show that the proposed algorithm outperformed other standard optimization algorithms in terms of accuracy and near-optimal kinetic parameter estimation. Nevertheless, the proposed algorithm is only expected to work well in

  11. Association of renal biochemical parameters with left ventricular diastolic dysfunction in a community-based elderly population in China: a cross-sectional study.

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    Jingmin Zhou

    Full Text Available BACKGROUND: Relationship of left ventricular diastolic dysfunction (LVDD with parameters that could provide more information than hemodynamic renal indexes has not been clarified. We aimed to explore the association of comprehensive renal parameters with LVDD in a community-based elderly population. METHODS: 1,166 community residents (aged ≥ 65 years, 694 females participating in the Shanghai Heart Health Study with complete data of renal parameters were investigated. Echocardiography was used to evaluate diastolic function with conventional and tissue Doppler imaging techniques. Serum urea, creatinine, urea-to-creatinine ratio, estimated glomerular filtration rate (eGFR and urinary albumin-to-creatinine ratio (UACR were analyzed on their associations with LVDD. RESULTS: The prevalence of LVDD increased in proportion to increasing serum urea, urea-to-creatinine ratio and UACR. These three renal parameters were found negatively correlated to peak early (E to late (A diastolic velocities ratio (E/A, and positively to left atrial volume index; UACR also positively correlated with E to peak early (E' diastolic mitral annular velocity ratio (E/E'. Serum urea, urea-to-creatinine ratio and UACR correlated with LVDD in logistic univariate regression analysis, and urea-to-creatinine ratio remained independently correlated to LVDD [Odds ratio (OR 2.82, 95% confidence interval (CI 1.34-5.95] after adjustment. Serum urea (OR 1.18, 95%CI 1.03-1.34, creatinine (OR 6.53, 95%CI 1.70- -25.02, eGFR (OR 0.22, 95%CI 0.07-0.65 and UACR (OR 2.15, 95%CI 1.42-3.24 were revealed independent correlates of advanced (moderate and severe LVDD. CONCLUSIONS: Biochemical parameters of renal function were closely linked with LVDD. This finding described new cardio-renal relationship in the elderly population.

  12. Leaf photosynthesis and respiration of three bioenergy crops in relation to temperature and leaf nitrogen: how conserved are biochemical model parameters among crop species?

    Science.gov (United States)

    Archontoulis, S. V.; Yin, X.; Vos, J.; Danalatos, N. G.; Struik, P. C.

    2012-01-01

    Given the need for parallel increases in food and energy production from crops in the context of global change, crop simulation models and data sets to feed these models with photosynthesis and respiration parameters are increasingly important. This study provides information on photosynthesis and respiration for three energy crops (sunflower, kenaf, and cynara), reviews relevant information for five other crops (wheat, barley, cotton, tobacco, and grape), and assesses how conserved photosynthesis parameters are among crops. Using large data sets and optimization techniques, the C3 leaf photosynthesis model of Farquhar, von Caemmerer, and Berry (FvCB) and an empirical night respiration model for tested energy crops accounting for effects of temperature and leaf nitrogen were parameterized. Instead of the common approach of using information on net photosynthesis response to CO2 at the stomatal cavity (An–Ci), the model was parameterized by analysing the photosynthesis response to incident light intensity (An–Iinc). Convincing evidence is provided that the maximum Rubisco carboxylation rate or the maximum electron transport rate was very similar whether derived from An–Ci or from An–Iinc data sets. Parameters characterizing Rubisco limitation, electron transport limitation, the degree to which light inhibits leaf respiration, night respiration, and the minimum leaf nitrogen required for photosynthesis were then determined. Model predictions were validated against independent sets. Only a few FvCB parameters were conserved among crop species, thus species-specific FvCB model parameters are needed for crop modelling. Therefore, information from readily available but underexplored An–Iinc data should be re-analysed, thereby expanding the potential of combining classical photosynthetic data and the biochemical model. PMID:22021569

  13. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Al-Okbi, S. Y.; Mohamed, D. A.; Donya, S. M.; Abd El Khalek, A. B.

    2011-07-01

    The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum) were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, {beta}-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory bio markers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures. (Author) 61 refs.

  14. [b][/b]Impact of copper (Cu at the dose of 50 mg on haematological and biochemical blood parameters in turkeys, and level of Cu accumulation in the selected tissues as a source of information on product safety for consumers

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    Bogusław Makarski

    2014-09-01

    Full Text Available Introduction. The current state-of the art points to a positive impact of copper (Cu supplements on the general health status in poultry. Copper induces beneficial changes in the haematological and biochemical blood parameters. It also displays immunostimulating properties and helps maintain a proper microbiological balance in the digestive tract. [b]Objective[/b]. The objective of this study was to investigate the impact of Cu at the dose of 50 mg/kg BW, administered in organic and inorganic form, on the haematological and biochemical blood parameters and level of Cu bioaccumulation in the liver and pectoral muscle. [b]Materials and method[/b]. The study was carried out on 45 BUT-9 turkeys which had been were reared for 16 weeks. They were divided into 3 experimental groups: I – the control group; II – fed with CuSO[sub]4[/sub] at the dose of 50 mg Cu•dm [sup]-3 [/sup]H[sub]2[/sub]O; III – received a Cu chelate with lysine at the same dose. [b]Results[/b]. The administration of Cu at the dose exceeding the nutritional recommendations did not induce beneficial changes in the examined birds. This indicates that it is not necessary to administer Cu doses higher than the recommended levels. The extent of Cu accumulation in the pectoral muscle increased by 40% compared to the control group, whereas in the liver it was higher by 30–35% than in the birds without Cu administration. The level of Cu in tissues does not pose a risk to consumers. [b]Conclusions[/b]. The supplementation of Cu at the dose of 50 mg has a negative impact on the level of the analyzed parameters. The results of the presented study indicate that the administered Cu dose exceeds birds’ demand for this element.

  15. The Effect of Crude Extract of Turbo coronatus from the Persian Gulf on Serum Biochemical Parameters and Hematiological Parameters of Rats

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    Amir Vazirizadeh

    2017-05-01

    Full Text Available : Turbotoxins are marine secondary metabolites that produce in Turbinidae family and were isolated from Japanese Turbo marmorata for the first time. A few research has been done on these metabolites so far. Another species, Turbo coronatus exists in Iran. The main aim of the current project was to investigate some biological effects of the crude extract of Turbo coronatus from the Persian Gulf . Materials and methods: In this study, 18 rats were selected in three groups including the control group. The experimental groups received ½ and 1/3 lethal doses intravenously and serum levels of liver and muscle enzymes, electrolytes and complete blood counts (CBC were measured after 24 hours. : The levels of liver and muscle enzymes, amylase, sodium, potassium, calcium, phosphorus, ferrous, haptoglobin, albumin and creatinine were significantly increased in experimental group compared with the control group by injection of crude extract of Turbo coronatus; however, hemoglobin, mean corpuscular volume (MCV, red blood cell count, magnesium, and glucose levels were significantly decreased in the experimental group compared with the control group. : Intravenous injection of ½ and 1/3 lethal doses of the crude extract of Turbo coronatus to rats produced rhabdomyolysis and hepatocytes injury. In addition, the crude extract injection acted as a haematoxin and decreased hemoglobin and MCV.

  16. Role of Bifidobacterium bifidum and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

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    Donya, S. M.

    2011-09-01

    Full Text Available The aim of the present research was to discover plant food extracts and probiotics that may have bioactivity towards chronic inflammation. Three plant food extract mixtures expected to be rich in phenolic compounds, carotenoids and tocopherols were prepared. The anti-inflammatory activity of the different mixtures as well as probiotic bacteria (Bifidobacterium bifidum were evaluated in adjuvant arthritis in rats. The anti-inflammatory effect, mechanism of action and safety of the three mixtures and Bifidobacterium bifidum were studied by measuring the size of inflammation and the determination of inflammatory and oxidative stress biomarkers, colonic bacteria profile and specific cytogenetic parameters. The contents of tocopherols, β-carotene and phenolic compounds in the mixtures were determined. The results show that the tested mixtures and Bifidobacterium bifidum possess promising anti-inflammatory effects. The mechanism of action seems to involve a reduction in oxidative stress and inflammatory biomarkers and an effect on colonic microflora. Genotoxicity and DNA fragmentation induced by adjuvant arthritis were prevented after supplementation with the tested mixtures.El objetivo de la presente investigación fue encontrar extractos de alimentos vegetales y probióticos que puedan tener bioactividad hacía la inflamación crónica. Mezclas de tres extractos de alimentos vegetales conocidos por su riqueza en compuestos fenólicos, carotenoides y tocoferoles han sido preparadas. La actividad anti-inflamatoria de las diferentes mezclas y de bacterias probióticas (Bifidobacterium bifidum fue evaluada en artritis adyuvante en ratas. El efecto anti-inflamatorio, mecanismo de acción y salubridad de las tres mezclas y de Bifidobacterium bifidum ha sido estudiado mediante la medida del tamaño de la inflamación y la determinación de biomarcadores de inflamación y estrés oxidativo, del perfil de bacterias del colón y de parámetros citogen

  17. Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis.

    Science.gov (United States)

    Alekish, Myassar O; Ismail, Zuhair B; Awawdeh, Mofleh S; Shatnawi, Shoroq

    2017-08-01

    The aims of this study were to evaluate the effects of intramammary infusion of sage ( Salvia officinalis ) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline), Group 2 (n=5): Amoxicillin alone (3 ml), and Group 3 (n=10): Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]). All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0) and at 24 (T24) and 48 (T48) h after the last treatment. The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (psage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (psage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations. In this study, the intramammary infusion of sage EO to ewes affected with

  18. Effects of alpha-lipoic acid supplementation on growth performance, antioxidant capacity and biochemical parameters for ammonia-exposed broilers.

    Science.gov (United States)

    Lu, Min; Bai, Jie; Wei, Fengxian; Xu, Bin; Sun, Quanyou; Li, Jie; Wang, Gaili; Tang, Xiangfang; Zhang, Hongfu; Yin, Qingqiang; Li, Shaoyu

    2017-08-01

    In order to estimate the effect of alpha-lipoic acid (LA) supplementation on relieving ammonia stress of broilers, 180 22-day-old male broilers were assigned to three groups, six replicates in each group and 10 birds per replicate. The three groups were: (1) a control group without ammonia stress; (2) exposure to 70 ppm atmospheric ammonia (AM); (3) exposure to 70 ppm atmospheric ammonia and administration of 300 mg/kg LA (AM + LA). The experimental period was 3 weeks. Results showed that average daily weight gain was increased and feed conversion ratio was decreased in the AM + LA group, compared with the AM group (P ammonia stress to restore broiler production performance to normal levels. © 2016 Japanese Society of Animal Science.

  19. Changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run in relation to the age and speed of runners

    Directory of Open Access Journals (Sweden)

    Zbigniew Jastrzębski

    2016-10-01

    Full Text Available Objectives: The objective of the study was to reveal morphology, electrolyte and chosen biochemical parameters in terms of health risk in runners in reference to their age and running speed in the case of running a distance of 100 km, which occur after 12 h or 24 h of recovery. Material and Methods: Fourteen experienced, male, amateur, ultra-marathon runners, divided into two age and two speed groups took part in the 100-km run. Blood samples for analyses indexes were collected from the ulnar vein just before the run, after 25 km, 50 km, 75 km and 100 km, as well as 12 h and 24 h after termination of the run. Results: The sustained ultramarathon run along with the distance covered (p < 0.05 caused an increase in myoglobin (max 90-fold, bilirubin (max 2.8-fold and total antioxidant status (max 1.15-fold, which also continued during the recovery. Significant changes in the number of white blood cells were observed with each sequential course and could be associated with muscle damage. The electrolyte showed changes towards slight hyperkalemia, but no changes in natrium and calcium concentrations. There were no significant differences between the age and speed groups for all the parameters after completing the 100-km run as well as after 12 h and 24 h of recovery. Conclusions: Considering changes in blood morphology and chosen biochemical parameters in ultra-marathon runners during a 100-km run it can be stated that such an exhausting effort may be dangerous for human health due to metabolic changes and large damage to the organs. Negative metabolic changes are independent of age of an ultramarathon runner and occur both in younger (32±5.33 years and older participants (50.56±9.7 years. It can be concluded that organ damage and negative metabolic changes during a 100-km run occur similarly in participants less experienced as well as in well trained runners. Int J Occup Med Environ Health 2016;29(5:801–814

  20. Effects of gallic acid on delta - aminolevulinic dehydratase activity and in the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats.

    Science.gov (United States)

    de Oliveira, Lizielle Souza; Thomé, Gustavo Roberto; Lopes, Thauan Faccin; Reichert, Karine Paula; de Oliveira, Juliana Sorraila; da Silva Pereira, Aline; Baldissareli, Jucimara; da Costa Krewer, Cristina; Morsch, Vera Maria; Chitolina Schetinger, Maria Rosa; Spanevello, Roselia Maria

    2016-12-01

    Diabetes mellitus (DM) is characterised by hyperglycaemia associated with the increase of oxidative stress. Gallic acid has potent antioxidant properties. The aim of this study was to evaluate the effect of gallic acid on the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats. Male rats were divided in groups: control, gallic acid, diabetic and diabetic plus gallic acid. DM was induced in the animals by intraperitoneal injection of streptozotocin (65mg/kg). Gallic acid (30mg/kg) was administered orally for 21days. Our results showed an increase in reactive species levels and lipid peroxidation, and a decrease in activity of the enzymes superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of the diabetic animals (PGallic acid treatment showed protective effects in these parameters evaluated, and also prevented a decrease in the activity of catalase and glutathione S-transferase, and vitamin C levels in the liver of diabetic rats. In addition, gallic acid reduced the number of nuclei and increased the area of the core in hepatic tissue, and increased the glomerular area in renal tissue. These results indicate that gallic acid can protect against oxidative stress-induced damage in the diabetic state. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  1. Effects of time-of-day on oxidative stress, cardiovascular parameters, biochemical markers, and hormonal response following level-1 Yo-Yo intermittent recovery test.

    Science.gov (United States)

    Aloui, K; Abedelmalek, S; Chtourou, H; Wong, D P; Boussetta, N; Souissi, N

    2017-03-01

    The aim of this study was to investigate the effect of time-of-day on oxidative stress, cardiovascular parameters, muscle damage parameters, and hormonal responses following the level-1 Yo-Yo intermittent recovery test (YYIRT). A total of 11 healthy subjects performed an intermittent test (YYIRT) at two times-of-day (i.e., 07:00 h and 17:00 h), with a recovery period of ≥36 h in-between, in a randomized order. Blood samples were taken at the rest (baseline) and immediately (post-YYIRT) after the YYIRT for measuring oxidative stress, biochemical markers, and hormonal response. Data were statistically analyzed using one-way and two-way repeated measures ANOVA and Bonferroni test at p creatine kinase (p  0.05) were similar for the morning and evening test. In conclusion, our findings suggest that aerobic performance presents diurnal variation with great result observed in the evening accompanied by an improvement of hormonal, metabolic, and oxidative responses. These data may help to guide athletes and coaches and contribute to public health recommendations on exercise and muscle damage particularly in the competitive periods.

  2. The influence of altitude and landforms on some biochemical and hematological parameters in Ouled Djellal ewes from arid area of South East Algeria.

    Science.gov (United States)

    Titaouine, Mohammed; Meziane, Toufik

    2015-01-01

    This study was conducted on Ouled Djellal ewes in arid area of south-east Algeria in order to reveal the influence of altitude and landforms on some hematological and biochemical parameters. A total of 160 ewes having 3-5 years of age, multiparous, non-pregnant, non-lactating and reared in arid areas of South East Algeria were included. Blood samples were divided according to factors of altitude and landform (plain region at 150 m above sea level, tableland region at 600 m above sea level and mountain region at 1000 m above sea level). The whole blood was analyzed for hematology, and plasma samples for biochemical analysis. The study found lowest glucose concentrations were detected in tableland region at 600 m. In plain region at 150 m, ewes had a higher (pewes at 1000 m and tableland ewes at 600 m were higher (pewes at 150 m. The highest calcium concentration was found at the altitude of 150 m and the lowest at the altitude of 1000 m (1.12±0.35 mmol/L vs. 0.52±0.03 mmol/L). Phosphorus levels were higher at altitudes of 150 m than at the altitude of 600 m and 1000 m (0.93±0.42 mmol/L vs. 0.68±0.54 mmol/L, 0.23±0.01 mmol/L). The highest hemoglobin concentration and value of hematocrit were detected in mountain ewes at the altitude of 1000 m (120.61 g/L, 40%) and the lowest at the altitude of 150 m (73.2 g/L, 31%) (pewes reared in arid area may be affected by altitude and landforms.

  3. Effects of sodium chloride salinity on ecophysiological and biochemical parameters of oak seedlings (Quercus robur L.) from use of de-icing salts for winter road maintenance.

    Science.gov (United States)

    Laffray, Xavier; Alaoui-Sehmer, Laurence; Bourioug, Mohamed; Bourgeade, Pascale; Alaoui-Sossé, Badr; Aleya, Lotfi

    2018-04-04

    Salt is widely used to melt snow on roads especially in mountain regions. Whether as rock salt or aerosols, spread or sprayed over road surfaces, salt may result in increased salt concentrations in soils, which, in turn, affect natural vegetation, especially tree seedlings already subjected to various other types of abiotic stress. The authors investigated the effects of salt treatment-related stress on seedling growth and certain biochemical parameters in Quercus robur to determine ion concentrations in root tips. Seedlings growing in a quartz sand/vermiculite mixture were subjected to NaCl concentrations of 0, 50, or 100 mM for 5 weeks. The results showed that high NaCl concentrations caused a marked reduction in total leaf biomass 55 and 75% for 50 and 100 mM treatments, respectively, in dry weight of stems (84%) and roots (175%) for 100 mM treatment and modified root architecture, whereas no changes appeared in leaf number. A non-significant decrease in relative water content, with changes in ion balance was recorded. Comparison of stressed to control plants show an increase in sodium (3.5-8-fold), potassium (0.6-fold), and chloride (9.5-14-fold) concentrations in the root tips while the K + /Na + ratio decreased. In taproots, no significant biochemical differences were observed between the salt-treated and the control plants for acid invertase activity, reducing sugars, sucrose, or soluble protein contents. The significance of ion and sugar accumulations in relation to osmotic adjustment and the ability of oak seedlings to cope with salt stress are discussed.

  4. Follow-up of some biochemical parameters to detect adaptive reactions induced in vivo by tritium contamination

    International Nuclear Information System (INIS)

    Petcu, I.; Moisoi, N.; Savu, D.; Constantinescu, B.

    1999-01-01

    The experiment intended to examine whether adaptive response could be elicited in vivo by low level internal contamination of rats with tritiated water and subsequently observed after a challenging irradiation with fast neutrons. Rats have been pre-contaminated for 3 weeks to total doses of 7 cGy and 35 cGy. Subsequently they were irradiated 1 Gy by fast neutrons obtained from deuterons (13.5 MeV) on a Be target. After 24 h the rats were sacrificed and the lipid peroxidation level was determined in liver, kidney, small intestine, spleen, bone marrow and plasma. The reduced glutathion and the glucose-6-phosphate dehydrogenase activity was determined in erythrocytes. Concurrently an in vivo assay was performed to observe the modifications of the thymidine intake in the isolated bone marrow cells. For the rats pre-exposed to tritiated water the lipid peroxides level was significantly decreased only for liver and kidney and only after the highest preirradiation dose (35 cGy). The thymidine incorporation assay revealed a putative adaptive reaction also for the 35 cGy preirradiated rats. The glutathion content was found to be increased (back to the normal level) for the tritium pre-contaminated and neutron irradiated animals as compared to those exposed only to fast neutrons. (authors)

  5. Effects of endurance and endurance-strength exercise on biochemical parameters of liver function in women with abdominal obesity.

    Science.gov (United States)

    Skrypnik, Damian; Ratajczak, Marzena; Karolkiewicz, Joanna; Mądry, Edyta; Pupek-Musialik, Danuta; Hansdorfer-Korzon, Rita; Walkowiak, Jarosław; Jakubowski, Hieronim; Bogdański, Paweł

    2016-05-01

    Obesity is a risk factor of nonalcoholic fatty liver disease. Although the standard therapy for obesity involves physical exercise, well-planned studies of the changes in liver function in response to different exercise intensities in obese subjects are scarce. The aim of the present study was to examine a question of how does exercise mode affect the liver function. 44 women with abdominal obesity were randomized into two exercise groups: endurance (group A) and endurance-strength (group B). Women in each group exercised for 60min 3 times/week for a 3-month period. Markers of liver function: serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltranspeptidase (GGT), alkaline phosphatase (ALP) activities, and bilirubin levels were quantified. We found significant differences in ALT (pexercise. Blood ALT and AST tended to decrease in group B, increase in group A. Significant reduction in serum GGT level after exercise in both groups was observed (pexercise led to changes in serum ALP activity and total or direct bilirubin level. However, endurance-strength training resulted in significant decreases in serum indirect bilirubin (pexercise (group B). The mode of exercise does matter: endurance-strength exercise led to a greater improvement, compared to endurance exercise, in the liver function in women with abdominal obesity. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  6. Effects of Bacillus subtilis on some physiological and biochemical parameters of Triticum aestivum L. (wheat) under salinity.

    Science.gov (United States)

    Lastochkina, Oksana; Pusenkova, Ludmila; Yuldashev, Ruslan; Babaev, Marat; Garipova, Svetlana; Blagova, Dar'ya; Khairullin, Ramil; Aliniaeifard, Sasan

    2017-12-01

    Endophytic strain Bacillus subtilis (B. subtilis) 10-4, producing indole-3-acetic acid (IAA) and siderofores but not active in phosphate solubilization, exerted a protective effect on Triticum aestivum L. (wheat) plant grown under salinity (2% NaCl) stress. Exposure to salt stress resulted in an essential increase of proline (Pro) and malondialdehyde (MDA) level in the seedlings. At the same time the seedlings inoculated with B. subtilis 10-4 were characterized by decreased level of stress-induced Pro and MDA accumulation. It was revealed that both B. subtilis 10-4 and salinity caused increase in the content of endogenous salicylic acid (SA) in wheat seedlings as compared to SA content in the control, while B. subtilis 10-4 suppressed stress-induced SA accumulation. Water storage capacity (WSC) in leaf tissues was increased and stress-induced hydrolysis of statolite starch in root cap cells of the germinal roots was reduced by B. subtilis 10-4. The obtained data indicated that the activation of the defense reactions induced by B. subtilis 10-4 induced defense reactions may be connected with their ability to decrease the level of stress-induced oxidative and osmotic stress in seedlings and with the increase of endogenous SA level that can make a significant contribution to the implementation of the protective effect of B. subtilis 10-4 and is manifested in the improvement of plant growth, WSC of leaves and slowing down of the process of statolite starch hydrolysis under salinity. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  7. Comparative study for different statistical models to optimize cutting parameters of CNC end milling machines

    International Nuclear Information System (INIS)

    El-Berry, A.; El-Berry, A.; Al-Bossly, A.

    2010-01-01

    In machining operation, the quality of surface finish is an important requirement for many work pieces. Thus, that is very important to optimize cutting parameters for controlling the required manufacturing quality. Surface roughness parameter (Ra) in mechanical parts depends on turning parameters during the turning process. In the development of predictive models, cutting parameters of feed, cutting speed, depth of cut, are considered as model variables. For this purpose, this study focuses on comparing various machining experiments which using CNC vertical machining center, work pieces was aluminum 6061. Multiple regression models are used to predict the surface roughness at different experiments.

  8. A comparative analysis of selected parameters of roofing used in the Polish construction industry

    Directory of Open Access Journals (Sweden)

    Radziszewska-Zielina Elżbieta

    2014-06-01

    Full Text Available Roofing is an important element in the construction of the roof. It is also one of the essential elements of the whole building. The choice of roofing should depend on technical parameters that affect the quality of the materials used and the price. The present paper is a comparative analysis of the properties of five roofing materials selected as examples with respect to twelve parameters. As can be seen from the comparative analysis of the roofing parameters, roofing tile is by far the best material, receiving the highest score in the ranking

  9. Study of the protective effect of ascorbic acid against the toxicity of stannous chloride on oxidative damage, antioxidant enzymes and biochemical parameters in rabbits.

    Science.gov (United States)

    Yousef, M I; Awad, T I; Elhag, F A; Khaled, F A

    2007-06-25

    Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS). Therefore, the present study has been carried out to investigate the antioxidant action of l-ascorbic acid (AA) in minimizing SnCl2 toxicity on lipid peroxidation, antioxidant enzyme, and biochemical parameters in male New Zealand white rabbits. Animals were assigned to one of four treatment groups: 0mg AA and 0mg SnCl2/kg BW (control); 40 mg AA/kg BW; 20mg SnCl2/kg BW; 20mg SnCl2 plus 40 mg AA/kg BW. Rabbits were orally administered the respective doses every other day for 12 weeks. Results obtained showed that SnCl2 significantly (Pacid-reactive substances (TBARS; the marker of lipid peroxidation) in plasma, while the activities of glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT), and the level of sulfhydryl groups (SH-group) were decreased (Pacid phosphatase (AcP) and lactate dehydrogenase (LDH) activities were decreased (Pcholesterol, triglyceride (TG), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL), glucose, urea and total bilirubin. On the other hand, the level of plasma high-density lipoprotein (HDL), total protein (TP), albumin (A) and globulin (G) were significantly (PAscorbic acid alone significantly decreased the levels of TBARS, lipids and urea, and increased the activities of GST, SOD and CAT, and the levels of SH-group and proteins. While the rest of the tested parameters were not affected. Also, the presence of AA with SnCl2 alleviated its harmful effects on most of the tested parameters. Therefore, the present results revealed that treatment with AA could minimize the toxic effects of stannous chloride.

  10. [Effect of consumption of bread with amaranth (Amaranthus dubius Mart. ex Thell.) on glycemic response and biochemical parameters in Sprague dawley rats].

    Science.gov (United States)

    Montero-Quintero, Keyla Carolina; Moreno-Rojas, Rafael; Molina, Edgar Alí; Colina-Barriga, Máximo Segundo; Sánchez-Urdaneta, Adriana Beatriz

    2014-11-01

    The incorporation of functional ingredients like amaranth (Amaranthus dubius Mart. ex Thell.) in bread making is a strategy to increase fiber intake, which is associated with beneficial health effects, improving glycemic response and lipid profile. Thirty male Sprague dawley rats were randomized into three groups: diet of bread with 0% amaranth (PA0, control), diet of bread with 10% amaranth (PA10) and bread diet with 20% amaranth (PA20) for determining the feed intake, weight gain, triglyceride, total cholesterol, VLDL-C, LDL-C, HDL-C, protein and postprandial glycemic response. Data were analyzed using a completely randomized with 10 replications analysis, using the comparison test of Tukey for biochemical parameters. Postprandial glycemic response was analyzed by the method of repeated measures over time. The daily intake and weight gain was not affected (P>0.05) in the groups with PA10 and PA20. The concentration of glucose, triglycerides and protein showed statistically significant differences (P>0.05) by the difference in content of amaranth diets. The values of total cholesterol, LDL-C, and atherogenic risk factor index were statistically significant (P. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  11. Evaluation of biochemical parameters and local and systemic levels of osteoactive and B-cell stimulatory factors in gestational diabetes in the presence or absence of gingivitis.

    Science.gov (United States)

    Gümüş, Pınar; Özçaka, Özgün; Ceyhan-Öztürk, Banu; Akcali, Aliye; Lappin, David F; Buduneli, Nurcan

    2015-03-01

    Gestational diabetes mellitus (GDM) is defined as varying glucose intolerance, with first onset or recognition in pregnancy. This study evaluates clinical and biochemical parameters in a possible association between GDM and gingivitis. A total of 167 pregnant females was included in the study. There were 101 females with GDM and 66 females without GDM. Subgroups were created according to the presence or absence of gingival inflammation. Plaque index, bleeding on probing, and probing depth were recorded at four sites per tooth. Serum, saliva, and gingival crevicular fluid (GCF) levels of interleukin (IL)-6, IL-8, soluble receptor activator of nuclear factor-kappa B ligand (sRANKL), osteoprotegerin (OPG), B-cell activating factor (BAFF), and a proliferation-inducing ligand (APRIL) were determined by enzyme-linked immunosorbent assay. Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests and Spearman correlation analysis. Age and anthropometric indices were higher in the GDM than non-GDM group (P gingivitis group than non-GDM with gingivitis group (P = 0.044). Serum and GCF BAFF (P gingivitis group than GDM without gingivitis group. The inflammatory response seems to be more pronounced in females with GDM. The observed increase in both local and systemic levels of inflammatory cytokines may suggest an interaction between gingivitis and GDM.

  12. Salivary caries parameters: Comparative study among Yemeni khat chewers and nonchewers

    OpenAIRE

    Khaled Rashad Al-Alimi; Abdul Aziz Abdul Razak; Roslan Saub

    2014-01-01

    Background/purpose: Millions of people in Yemen and East African countries chew khat for more than 5 hours daily for its amphetamine-like effects. Previous studies have associated khat chewing with salivary glands enlargement, inflammation, and xerostomia. However, no information is available on the possible effects of this habit on salivary parameters. This comparative study aims to evaluate salivary parameters, such as salivary flow rate, pH, and viscosity among Yemeni khat chewers and nonc...

  13. Effect of Milk Thistle (Silybum marianum L. on Biochemical Parameters and Immunity of Broiler Chicks Fed Aflatoxin B1 after Three Weeks

    Directory of Open Access Journals (Sweden)

    Maliheh Amiri Dumari

    2014-09-01

    Full Text Available Background: This study was conducted to determine the efficacy of milk thistle seeds (MTSs in counteracting the toxic effects of aflatoxin B1 (AFB1 in a contaminated diet fed to broilers. Methods: Two dietary inclusion rates of AFB1 (0, 0.250 and 500 ppb and MTS (0, 0.5 and 1% were tested in a 3×3 factorial manner. The effect of nine experimental treatments was assessed using 216 one-d-old Ross 308 male broiler chicks in a randomized complete design with 4 replicates of 6 birds each from one to 21 days of age. The effects of dietary AFB1 and MTS on serum biochemistry factors, antibody titer against Newcastle disease (ND and influenza disease (ID in broilers were evaluated at the end of this period. Results: Statistical analysis of the main effects of diets indicated no significant changes in uric acid, cholesterol, triglycerides, low density lipoprotein (LDL, ID, and phosphorus compared to the control (P>0.01. Also, addition of 500 ppb of dietary AFB1 into the diet was associated with significant decreases in serum glucose, calcium, high density lipoprotein (HDL, and ND compared to the control group (P<0.01. The contaminated diet significantly increased the activities of aspartate aminotransferase (AST and alanine aminotransferase (ALT (P<0.05. Conclusion: Milk thistle showed protective effects and resulted in some serum enzyme activities and serum biochemical changes associated with aflatoxin toxicity.

  14. Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations.

    Science.gov (United States)

    Kühn, I; Allestam, G; Stenström, T A; Möllby, R

    1991-01-01

    A simple, automated microplate system for biochemical characterization of water isolates can be used to obtain fingerprints of the bacterial flora from various water samples. Mathematical models for calculating the diversities and similarities between bacterial populations are described for such fingerprints. The diversity may give information on whether an indigenous or allochthonous flora is present, and the similarities between bacterial populations, as calculated by using a population similarity coefficient (Sp), may indicate contaminations between different water samples. The system was demonstrated on coliform bacterial populations from various water samples, with or without suspected intercontamination. For unrelated water samples, the Sps were close to 0, whereas repeated samples of the same source showed Sps of 0.64 to 0.74. The Sp values from several water samples were also clustered to form a dendrogram, thus indicating the relative similarities between the bacterial populations to confirm suspected common sources of pollution. PMID:1781680

  15. Elastin in the human intervertebral disk. A histological and biochemical study comparing it with elastin in the human yellow ligament.

    Science.gov (United States)

    Mikawa, Y; Hamagami, H; Shikata, J; Yamamuro, T

    1986-01-01

    The elastic fiber and elastin in the human yellow ligament and intervertebral disk were studied histologically and biochemically. The elastic fiber in the human intervertebral disk, which until now had not been clearly identified microscopically, was observed clearly. We found the distribution of the elastic fiber in the intervertebral disk to be very sparse and irregular, and its diameter was small, being about one-tenth of that found in the yellow ligament. The elastin contents of the yellow ligament and intervertebral disk were 46.7% +/- 0.9% and 1.7% +/- 0.2% respectively (mean +/- SE) of the total dry weight. The amino acid composition of elastin in the yellow ligament is similar to that of other tissue, as reported in the literature; however, that found in the intervertebral disk is significantly different. It would appear, therefore, that the elastin in the intervertebral disk is of a different type from that found elsewhere.

  16. Comparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group).

    Science.gov (United States)

    Kamdem, Irénée; Jacquet, Nicolas; Tiappi, Florian Mathias; Hiligsmann, Serge; Vanderghem, Caroline; Richel, Aurore; Jacques, Philippe; Thonart, Philippe

    2015-11-01

    The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the results of a biochemical analysis performed on six combined morphological parts of Williams Cavendish Lignocellulosic Biomass (WCLB) after steam cracking (SC) and steam explosion (SE) pretreatments. Solid (S) and liquid (L) fractions (Fs) obtained from SC pretreatment performed at 180°C (SLFSC180) and 210°C (SLFSC210) generated, after diluted acid hydrolysis, the highest proportions of neutral sugar (NS) contents, specifically 52.82 ± 3.51 and 49.78 ± 1.39%w/w WCLB dry matter (DM), respectively. The highest proportions of glucose were found in SFSC210 (53.56 ± 1.33%w/w DM) and SFSC180 (44.47 ± 0.00%w/w DM), while the lowest was found in unpretreated WCLB (22.70 ± 0.71%w/w DM). Total NS content assessed in each LF immediately after SC and SE pretreatments was less than 2%w/w of the LF DM, thus revealing minor acid autohydrolysis consequently leading to minor NS production during the steam pretreatment. WCLB subjected to SC at 210 °C (SC210) generated up to 2.7-fold bioaccessible glucan and xylan. SC and SE pretreatments showed potential for the deconstruction of WCLB (delignification, depolymerization, decrystallization and deacetylation), enhancing its enzymatic hydrolysis. The concentrations of enzymatic inhibitors, such as 2-furfuraldehyde and 5-(hydroxymethyl)furfural from LFSC210, were the highest (41 and 21 µg ml(-1), respectively). This study shows that steam pretreatments in general and SC210 in particular are required for efficient bioconversion of WCLB. Yet, biotransformation through biochemical processes (e.g., anaerobic digestion) must be performed to assess the efficiency of these pretreatments. © The Author(s) 2015.

  17. Zoledronic acid as compared with observation in multiple myeloma patients at biochemical relapse: results of the randomized AZABACHE Spanish trial

    Science.gov (United States)

    García-Sanz, Ramón; Oriol, Albert; Moreno, María J.; de la Rubia, Javier; Payer, Angel R.; Hernández, Miguel T.; Palomera, Luis; Teruel, Ana I.; Blanchard, María J.; Gironella, Mercedes; Ribas, Paz; Bargay, Joan; Abellá, Eugenia; Granell, Miquel; Ocio, Enrique M.; Ribera, Josep M.; San Miguel, Jesús F.; Mateos, María V.

    2015-01-01

    This study analyzed the anti-myeloma effect of zoledronic acid monotherapy by investigating patients at the time of asymptomatic biochemical relapse. One hundred patients were randomized to receive either zoledronic acid (4 mg iv/4 weeks, 12 doses) (n=51) or not (n=49). Experimental and control groups were well balanced for disease and prognostic features. Zoledronic acid did not show an antitumor effect according to changes in M-component. However, there were fewer symptomatic progressions in the experimental group than in the control group (34 versus 41, respectively; P=0.05) resulting in a median time to symptoms of 16 versus 10 months (P=0.161). The median time to next therapy was also slightly longer for the treated group than the untreated, control group (13.4 versus 10.1 months), although the difference was not statistically significant (P=0.360). The pattern of relapses was different for treated versus control patients: progressive bone disease (8 versus 20), anemia (24 versus 18), renal dysfunction (1 versus 2), and plasmacytomas (1 versus 1, respectively). This concurred with fewer skeletal-related events in the treated group than in the control group (2 versus 14), with a projected 4-year event proportion of 6% versus 40% (P<0.001). In summary, zoledronic acid monotherapy does not show an antitumor effect on biochemical relapses in multiple myeloma, but does reduce the risk of progression with symptomatic bone disease and skeletal complications. This trial was registered in the ClinicalTrials.gov database with code NCT01087008 PMID:26069291

  18. [The influence of N-, S-containing chinasolone derivatives (NC-224) on the biochemical and physicochemical parameters of membrane endoplasmatic reticulum and nuclear chromatine fractions of rats liver cells in conditions of its injury by tetrachloromethane].

    Science.gov (United States)

    Gubs'kyî, Iu I; Goriushko, G G; Belenichev, I F; Kovalenko, S I; Litvinova, N V; Marchenko, O M; Kurapova, T M; Babenko, L P; Velychko, O M

    2010-01-01

    Using biochemical and physicochemical methods of investigation in vivo, the effect of the substance NC-224, N-, S-chinasolone-derivative, on the lipoperoxidation activity in rat liver endoplasmatic reticulum membranes and nuclear chromatin fractions under tetrachloromethane intoxication have been studied. It was shown that NC-224 has pronounced antioxidant activity which is the biochemical basis of the substance membrane- and genome-protective effects and its ability to restore physicochemical properties of the surface and hydrophobic zones of hepatocyte membranes and structural parameter nuclear chromatin fractions in the conditions of chemical liver injury.

  19. Gamma radiation in some microbiological and biochemical parameters of ethanolic fermentation.; Efeito da radiacao gama em alguns parametros microbiologicos e bioquimicos da fermentacao alcoolica

    Energy Technology Data Exchange (ETDEWEB)

    Alcarde, Andre Ricardo

    2000-07-01

    The objective of this work was to evaluate the effect of gamma radiation in reducing the bacterial population of the sugar cane must and verify its influence in the ethanolic fermentation. For this purpose, some microbiological and biochemical parameters of the ethanolic fermentation were analyzed, such as bacterial count; viability, replication and living replicates of the yeast; p H, acidity (total and volatile), glycerol and production of organic acids (acetic, lactic and succinic) during the fermentation; and fermentative yield. Bacteria of the genera Bacillus and Lactobacillus are the most common contaminants of the ethanolic fermentation and they might cause a decrease in the fermentative yield. The ionizing radiations may affect the microorganisms altering the DNA of the cells, which lose the ability to reproduce themselves and die. The experimental design was in randomized blocks (three) with one replicate in each block. The must was sugar-cane juice with approximately 5% of total reducing sugar. Bacteria of the following species were tested: Bacillus subtilis, Bacillus coagulans, Lactobacillus plantarum and Lactobacillus fermentum. The experiments were the inoculation of each bacteria separately in the must, the inoculation of the mixture of the four bacteria in the must and the use of natural sugar-cane juice with its own contaminating microorganisms. The contaminated must was irradiated with the doses of 0.0 (control), 2.0,4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation (60-Cobalt) at an average rate of 2.0 kGy/h. After the irradiation, the fermentation of the must was carried out using the yeast Saccharomyces cerevisiae (Fleischmann). It was also accomplished an experiment with the inoculation of the mixture of the four bacteria in the must and, instead of using gamma radiation to decontaminate the must, it was used the antimicrobial Kamoran ID in the concentration of 3 ppm. The effects of the irradiation of the must were: reduction of the bacterial

  20. Binocular video ophthalmoscope for simultaneous recording of sequences of the human retina to compare dynamic parameters

    Science.gov (United States)

    Tornow, Ralf P.; Milczarek, Aleksandra; Odstrcilik, Jan; Kolar, Radim

    2017-07-01

    A parallel video ophthalmoscope was developed to acquire short video sequences (25 fps, 250 frames) of both eyes simultaneously with exact synchronization. Video sequences were registered off-line to compensate for eye movements. From registered video sequences dynamic parameters like cardiac cycle induced reflection changes and eye movements can be calculated and compared between eyes.

  1. EFFECTS OF L-ASCORBIC ACID AND ALPHA-TOCOPHEROL ON BIOCHEMICAL PARAMETERS OF SWIMMING-INDUCED OXIDATIVE STRESS IN SERUM OF GUINEA PIGS.

    Science.gov (United States)

    Bursać-Mitrović, Marija; Milovanović, Dragan R; Mitić, Radoslav; Jovanović, Danijela; Sovrlić, Miroslav; Vasiljević, Perica; Tomović, Jovica; Manojlović, Nedeljko

    2016-01-01

    The purpose of this study is to determine the effect of L-ascorbic acid and alpha-tocopherol as well as combination of these vitamins with or without exposure to physical exercise on intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase in the serum of guinea pigs. The experimental measurements of intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase were done in the serum of guinea pigs. The animals were exposed to the test load to achieve exhaustion and the test was terminated when the animal for the third time to sink into the water. The results of this study demonstrated that endurance exercise of guinea pigs induced oxidative stress response in terms of increased lipid peroxidation and activity of xanthine oxidase in the serum of experimental animals. Our study investigated the antioxidant activity of L-ascorbic acid and alpha-tocopherol also measuring three protective markers in the serum: total antioxidant activity, content of glutathione and activity of catalase. The results obtained show that the vitamins influence the concentrations of above mentioned biochemical parameters, which points out their protective effect of swimming-induced oxidative stress. Single or combined administration of L-ascorbic acid and alpha-tocopherol caused significant inhibition of these markers indicating the important antioxidant activity of the vitamins. Results lead to conclude that the combined treatments with vitamins with or without exposure to physical exercise showed the clear synergistic effect..

  2. The effect of Opuntia ficus-indica juice supplementation on oxidative stress, cardiovascular parameters, and biochemical markers following yo-yo Intermittent recovery test.

    Science.gov (United States)

    Khouloud, Aloui; Abedelmalek, Salma; Chtourou, Hamdi; Souissi, Nizar

    2018-03-01

    The aim of the study was to investigate the effect of a flavonoid-rich fresh fruit juice on cardiovascular, oxidative stress, and biochemical parameters during the yo-yo intermittent recovery test (YYIRT). Twenty-two healthy males subjects participated in this study divided into two groups: An experimental group (EG: n  = 11) who consumed the antioxidant supplement and a control group (CG: n  = 11). All participants performed two test sessions at 07:00 hr before and after 2 weeks of supplementation with Opuntia ficus-indica juice. Blood samples were taken before (P1) and immediately (P2) after the YYIRT. Our results showed that following the 2,2-diphenyl-1- picrylhydrazyl (DPPH ● ) test, the Opuntia ficus-indica juice has an antioxidant capacity for capturing free radicals ( p  Opuntia ficus-indica juice before and immediately after YYIRT. However, no significant effect on HDL ( p  > .05), GLC ( p  > .05) levels nor the SBP and DBP ( p  > .05) was observed after supplementation with Opuntia ficus-indica juice. The supplementation leads to an improvement on YYIRT performance (The total distance covered during the YYIRT, V O 2max , VMA) and the rating of perceived exertion (RPE). Opuntia ficus-indica juice has a potent antioxidant activity that reduces total and LDL-cholesterol with only a moderate lowering of HDL-cholesterol and oxidative stress. Moreover, supplementation decreases muscle damage caused by the endurance exercise.

  3. Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio by Vitamin C and Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Banaee

    2015-12-01

    Full Text Available The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg/L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg/kg diet, vitamin C (1000 mg/kg diet or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA as well as biochemical parameters, including the activity of aspartate aminotransferase (AST, alanine aminotransferase (ALT, creatine phosphokinase (CPK, lactate dehydrogenase (LDH, and acetylcholinesterase (AChE were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium-treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.

  4. Influence of Dietary Zinc and Vitamin C Supplementation on Some Blood Biochemical Parameters and Egg Production in Free-Range Laying Hens

    Directory of Open Access Journals (Sweden)

    Vasko GERZILOV

    2015-03-01

    Full Text Available The study aimed to follow out the effect of antistress dietary supplements Zinteral 35 and vitamin C on the levels of some blood biochemical parameters (corticosterone, total cholesterol, glucose, total protein and creatinine and egg production in laying hens during cold (7o C, thermoneutral (19o C and hot (31o C periods. The fowls were divided in three groups (26 females and 3 males in each group. They were reared in a free-range management system with elements of organic production. The experimental treatments were as followed: first (control group without dietary supplement, second group with 100 mg Zinteral 35 per kg diet containing 35 mg/kg zinc oxide, third group with the same amount of Zinteral 35 together with 250 mg vitamin C per kg diet. During the three periods with different ambient temperature, the hens supplemented either with zinc alone (second group or co-administered zinc + vitamin C (third group had significantly lower levels of plasma corticosterone (P<0.001, serum cholesterol (P<0.05 and glucose (P<0.05 than those from the first (control group. The differences between the third and the first groups were bigger versus those between the second and the first groups. For the entire period (March 1 and June 21, egg production was higher by 2.22 % and 4.60 % in the second and third groups respectively in comparison to the first group. The combination of 100 mg Zinteral 35 and 250 mg vitamin C per 1 kg diet exhibited a synergistic effect in reducing cold and heat stress in laying hens and increased their egg production.

  5. Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

    Science.gov (United States)

    Tienken, Reka; Kersten, Susanne; Frahm, Jana; Hüther, Liane; Meyer, Ulrich; Huber, Korinna; Rehage, Jürgen; Dänicke, Sven

    2015-01-01

    Simple Summary Several biological changes occur during the transition from late pregnancy to early lactation which is associated with a high susceptibility of health disorders. Nicotinic acid, as feed additive, is suggested to balance catabolic metabolism of periparturient dairy cows by attenuating lipolysis and impact production performance. This study provides information of the biological changes occurring around parturition with special emphasis on differences between primiparous and multiparous cows. Present results showed that energy-dense feeding prepartum did not result in metabolic imbalances postpartum in dairy cows which were similar in body condition score. Nicotinic acid supplementation did not reveal any effect. Abstract The periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA) combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010) serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL), whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of

  6. Effects of Processing of Starter Diets on Performance, Nutrient Digestibility, Rumen Biochemical Parameters and Body Measurements of Brown Swiss Dairy Calves

    Directory of Open Access Journals (Sweden)

    J. Ghassemi Nejad

    2012-07-01

    Full Text Available In order to investigate the effect of physical forms of starter diets on performance, weaning age, nutrient digestibility and rumen biochemical factors, 24 female of neonatal Brown Swiss calves (average body weight of 39.5±1.2 kg were randomly assigned to three treatments. Dietary treatments were mashed (MS, pelleted (PS, and texturized (TS starter using 8 calves from birth till 90 days of age in each treatment. Diets were formulated to be iso-nitrogenous with 21% crude protein. Based on the experimental results, calves that received PS and TS diets, had significant higher average daily gain (ADG than those receiving MS (p0.05. Ruminal pH was higher (p<0.01 in MS than the other groups, but ruminal ammonia (g/dl concentration was not different among the treatments. Body measurements such as body length, pin width, hip width, pin to hip length, size of metacarpus and metatarsus bones, hip height, wither height, stomach size and heart girth were not significantly different among the treatments. Overall, it is concluded that starter diets in the form of pellet and texture can improve performance in neonatal Brown Swiss calves compared to the mashed form.

  7. Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats.

    Science.gov (United States)

    Amin, K A; Abdel Hameid, H; Abd Elsttar, A H

    2010-10-01

    Tartrazine and carmoisine are an organic azo dyes widely used in food products, drugs and cosmetics. The present study conducted to evaluate the toxic effect of these coloring food additives; on renal, hepatic function, lipid profile, blood glucose, body-weight gain and biomarkers of oxidative stress in tissue. Tartrazine and carmoisine were administered orally in two doses, one low and the other high dose for 30 days followed by serum and tissue sample collection for determination of ALT, AST, ALP, urea, creatinine, total protein, albumin, lipid profile, fasting blood glucose in serum and estimation of GSH, catalase, SOD and MDA in liver tissue in male albino rat. Our data showed a significant increase in ALT, AST, ALP, urea, creatinine total protein and albumin in serum of rats dosed with tartrazine and carmoisine compared to control rats and these significant change were more apparent in high doses than low, GSH, SOD and Catalase were decreased and MDA increased in tissue homogenate in rats consumed high tartrazine and both doses of carmoisine. We concluded that tartrazine and carmoisine affect adversely and alter biochemical markers in vital organs e.g. liver and kidney not only at higher doses but also at low doses. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

  8. Effects of dietary administration of stinging nettle (Urtica dioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila.

    Science.gov (United States)

    Ngugi, Charles C; Oyoo-Okoth, Elijah; Mugo-Bundi, James; Orina, Paul Sagwe; Chemoiwa, Emily Jepyegon; Aloo, Peninah A

    2015-06-01

    We investigated effects of dietary administration of stinging nettle (Urtica dioica) on growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) against Aeromonas hydrophila. Fish were divided into 4 groups and fed for 4 and 16 weeks with 0%, 1%, 2% and 5% of U. dioica incorporated into the diet. Use of U. dioica in the diet resulted in improved biochemical, hematological and immunological parameters. Among the biochemical parameters; plasma cortisol, glucose, triglyceride and cholesterol decreased while total protein and albumin in fish increased with increasing dietary inclusion of U. dioica. Among the haematology parameters: red blood cell (RBC), white blood cell (WBC) counts, haematocrit (Htc), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and netrophiles increased with increasing dietary inclusion levels of U. dioica, some depending on the fish age. Serum immunoglobulins, lysozyme activity and respiratory burst were the main immunological parameters in the adult and juvenile L. victorianus measured and they all increased with increasing herbal inclusion of U. dioica in the diet. Dietary incorporation of U. dioica at 5% showed significantly higher relative percentage survival (up to 95%) against A. hydrophila. The current results demonstrate that using U. dioica can stimulate fish immunity and make L. victorianus more resistant to bacterial infection (A. hydrophila). Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Parameter estimation method that directly compares gravitational wave observations to numerical relativity

    Science.gov (United States)

    Lange, J.; O'Shaughnessy, R.; Boyle, M.; Calderón Bustillo, J.; Campanelli, M.; Chu, T.; Clark, J. A.; Demos, N.; Fong, H.; Healy, J.; Hemberger, D. A.; Hinder, I.; Jani, K.; Khamesra, B.; Kidder, L. E.; Kumar, P.; Laguna, P.; Lousto, C. O.; Lovelace, G.; Ossokine, S.; Pfeiffer, H.; Scheel, M. A.; Shoemaker, D. M.; Szilagyi, B.; Teukolsky, S.; Zlochower, Y.

    2017-11-01

    We present and assess a Bayesian method to interpret gravitational wave signals from binary black holes. Our method directly compares gravitational wave data to numerical relativity (NR) simulations. In this study, we present a detailed investigation of the systematic and statistical parameter estimation errors of this method. This procedure bypasses approximations used in semianalytical models for compact binary coalescence. In this work, we use the full posterior parameter distribution for only generic nonprecessing binaries, drawing inferences away from the set of NR simulations used, via interpolation of a single scalar quantity (the marginalized log likelihood, ln L ) evaluated by comparing data to nonprecessing binary black hole simulations. We also compare the data to generic simulations, and discuss the effectiveness of this procedure for generic sources. We specifically assess the impact of higher order modes, repeating our interpretation with both l ≤2 as well as l ≤3 harmonic modes. Using the l ≤3 higher modes, we gain more information from the signal and can better constrain the parameters of the gravitational wave signal. We assess and quantify several sources of systematic error that our procedure could introduce, including simulation resolution and duration; most are negligible. We show through examples that our method can recover the parameters for equal mass, zero spin, GW150914-like, and unequal mass, precessing spin sources. Our study of this new parameter estimation method demonstrates that we can quantify and understand the systematic and statistical error. This method allows us to use higher order modes from numerical relativity simulations to better constrain the black hole binary parameters.

  10. The AmP project: Comparing species on the basis of dynamic energy budget parameters.

    Directory of Open Access Journals (Sweden)

    Gonçalo M Marques

    2018-05-01

    Full Text Available We developed new methods for parameter estimation-in-context and, with the help of 125 authors, built the AmP (Add-my-Pet database of Dynamic Energy Budget (DEB models, parameters and referenced underlying data for animals, where each species constitutes one database entry. The combination of DEB parameters covers all aspects of energetics throughout the full organism's life cycle, from the start of embryo development to death by aging. The species-specific parameter values capture biodiversity and can now, for the first time, be compared between animals species. An important insight brought by the AmP project is the classification of animal energetics according to a family of related DEB models that is structured on the basis of the mode of metabolic acceleration, which links up with the development of larval stages. We discuss the evolution of metabolism in this context, among animals in general, and ray-finned fish, mollusks and crustaceans in particular. New DEBtool code for estimating DEB parameters from data has been written. AmPtool code for analyzing patterns in parameter values has also been created. A new web-interface supports multiple ways to visualize data, parameters, and implied properties from the entire collection as well as on an entry by entry basis. The DEB models proved to fit data well, the median relative error is only 0.07, for the 1035 animal species at 2018/03/12, including some extinct ones, from all large phyla and all chordate orders, spanning a range of body masses of 16 orders of magnitude. This study is a first step to include evolutionary aspects into parameter estimation, allowing to infer properties of species for which very little is known.

  11. Comparative analysis and validation of the malachite green assay for the high throughput biochemical characterization of terpene synthases.

    Science.gov (United States)

    Vardakou, Maria; Salmon, Melissa; Faraldos, Juan A; O'Maille, Paul E

    2014-01-01

    Terpenes are the largest group of natural products with important and diverse biological roles, while of tremendous economic value as fragrances, flavours and pharmaceutical agents. Class-I terpene synthases (TPSs), the dominant type of TPS enzymes, catalyze the conversion of prenyl diphosphates to often structurally diverse bioactive terpene hydrocarbons, and inorganic pyrophosphate (PPi). To measure their kinetic properties, current bio-analytical methods typically rely on the direct detection of hydrocarbon products by radioactivity measurements or gas chromatography-mass spectrometry (GC-MS). In this study we employed an established, rapid colorimetric assay, the pyrophosphate/malachite green assay (MG), as an alternative means for the biochemical characterization of class I TPSs activity.•We describe the adaptation of the MG assay for turnover and catalytic efficiency measurements of TPSs.•We validate the method by direct comparison with established assays. The agreement of k cat/K M among methods makes this adaptation optimal for rapid evaluation of TPSs.•We demonstrate the application of the MG assay for the high-throughput screening of TPS gene libraries.

  12. A comparison of the effects of Portulaca oleracea seeds hydro-alcoholic extract and Vitamin C on biochemical, hemodynamic and functional parameters in cardiac tissue of rats with subclinical hyperthyroidism

    Science.gov (United States)

    Khodadadi, Hadi; Pakdel, Roghayeh; Khazaei, Majid; Niazmand, Said; Bavarsad, Kowsar; Hadjzadeh, Mousa AL-Reza

    2018-01-01

    Objective: The present study was performed to evaluate the effects of hydro-alcoholic extract of Portulaca oleracea (P. oleracea) seeds and Vitamin C on biochemical and hemodynamic parameters in cardiac tissue of rats with subclinical hyperthyroidism. Materials and Methods: Forty eight male rats were divided into six groups of 8 and treated for 4 weeks. T4 group received daily injection of levothyroxine sodium (20 μg/kg) and control group was given daily injection of saline. T4-Po groups were given T4 plus 100, 200, and 400 mg/kg of P. oleracea seeds extract in drinking water daily. T4-Vit C group received T4 plus daily injection of Vitamin C (100 mg/kg). At the end of the experiment, body weight, serum free T4 level, left ventricular developed pressure (LVDP), malondialdehyde (MDA) and total thiol levels were measured. Results: Free T4 levels were increased in all groups that were treated with T4. Weight gain was decreased in T4 and T4-Po100 groups compared to control group (p<0.001 and p<0.05). However, body weight was increased in T4-Po (200 and 400) and T4-Vit C groups compared to T4 group. LVDP was increased in T4 group compared to control group but, LVDP was decreased in T4-Po and T4-Vit C groups. Malondialdehyde was decreased in T4-Po groups and T4-Vit C group compared to T4 group. Total thiol groups were increased in T4-Po (200 and 400) and T4-Vit C groups compared to T4 group. Conclusion: The results showed that P. oleracea extract has a protective effect on cardiac dysfunction due to subclinical hyperthyroidism induced by levothyroxine sodium in rats. PMID:29632847

  13. Biochemical methane potential prediction of plant biomasses: Comparing chemical composition versus near infrared methods and linear versus non-linear models.

    Science.gov (United States)

    Godin, Bruno; Mayer, Frédéric; Agneessens, Richard; Gerin, Patrick; Dardenne, Pierre; Delfosse, Philippe; Delcarte, Jérôme

    2015-01-01

    The reliability of different models to predict the biochemical methane potential (BMP) of various plant biomasses using a multispecies dataset was compared. The most reliable prediction models of the BMP were those based on the near infrared (NIR) spectrum compared to those based on the chemical composition. The NIR predictions of local (specific regression and non-linear) models were able to estimate quantitatively, rapidly, cheaply and easily the BMP. Such a model could be further used for biomethanation plant management and optimization. The predictions of non-linear models were more reliable compared to those of linear models. The presentation form (green-dried, silage-dried and silage-wet form) of biomasses to the NIR spectrometer did not influence the performances of the NIR prediction models. The accuracy of the BMP method should be improved to enhance further the BMP prediction models. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Corrective effects of acerola (Malpighia emarginata DC.) juice intake on biochemical and genotoxical parameters in mice fed on a high-fat diet.

    Science.gov (United States)

    Leffa, Daniela Dimer; da Silva, Juliana; Daumann, Francine; Dajori, Ana Luiza Formentin; Longaretti, Luiza Martins; Damiani, Adriani Paganini; de Lira, Fabio; Campos, Fernanda; Ferraz, Alexandre de Barros Falcão; Côrrea, Dione Silva; de Andrade, Vanessa Moraes

    2014-12-01

    Acerola contains high levels of vitamin C and rutin and shows the corresponding antioxidant properties. Oxidative stress on the other hand is an important factor in the development of obesity. In this study, we investigated the biochemical and antigenotoxic effects of acerola juice in different stages of maturity (unripe, ripe and industrial) and its main pharmacologically active components vitamin C and rutin, when given as food supplements to obese mice. Initial HPLC analyses confirmed that all types of acerola juice contained high levels of vitamin C and rutin. DPPH tests quantified the antioxidant properties of these juices and revealed higher antioxidant potentials compared to pure vitamin C and rutin. In an animal test series, groups of male mice were fed on a standard (STA) or a cafeteria (CAF) diet for 13 weeks. The latter consisted of a variety of supermarket products, rich in sugar and fat. This CAF diet increased the feed efficiency, but also induced glucose intolerance and DNA damage, which was established by comet assays and micronucleus tests. Subsequently, CAF mice were given additional diet supplements (acerola juice, vitamin C or rutin) for one month and the effects on bone marrow, peripheral blood, liver, kidney, and brain were examined. The results indicated that food supplementation with ripe or industrial acerola juice led to a partial reversal of the diet-induced DNA damage in the blood, kidney, liver and bone marrow. For unripe acerola juice food supplementation, beneficial effects were observed in blood, kidney and bone marrow. Food supplementation with vitamin C led to decreased DNA damage in kidney and liver, whereas rutin supplementation led to decreased DNA damage in all tissue samples observed. These results suggest that acerola juice helps to reduce oxidative stress and may decrease genotoxicity under obesogenic conditions. Copyright © 2014 Elsevier B.V. All rights reserved.

  15. p.H1069Q mutation in ATP7B and biochemical parameters of copper metabolism and clinical manifestation of Wilson's disease.

    Science.gov (United States)

    Gromadzka, Graznya; Schmidt, Harmut H J; Genschel, Janine; Bochow, Bettina; Rodo, M; Tarnacka, Beatek; Litwin, Thomas; Chabik, Grzegorz; Członkowska, Anna

    2006-02-01

    We compared the effect of the p.H1069Q mutation and other non-p.H1069Q mutations in ATP7B on the phenotypic expression of Wilson's disease (WD), and assessed whether the clinical phenotype of WD in compound heterozygotes depends on the type of mutation coexisting with the p.H1069Q. One hundred forty-two patients with clinically, biochemically, and genetically diagnosed WD were studied. The mutational analysis of ATP7B was performed by direct sequencing. A total number of 26 mutations in ATP7B were identified. The p.His1069Gln was the most common mutation (allelic frequency: 72%). Seventy-three patients were homozygous for this mutation. Of compound heterozygotes, 37 had frameshift/nonsense mutation, and 20 had other missense mutation on one of their ATP7B alleles. Twelve patients had two non-p.H1069Q mutations. Patients homozygous for the p.H1069Q mutation had the less severe disturbances of copper metabolism and the latest presentation of first WD symptoms. The most severely disturbed copper metabolism and the earliest age at initial disease manifestation was noticed in non-p.H1069Q patients. In compound heterozygotes, the type of mutation coexisting with the p.H1069Q to a small extent influenced WD phenotype. The phenotype of WD varied considerably among patients with the same genotype. The p.H1069Q mutation is associated with late WD manifestation and with a mild disruption of copper metabolism. In compound heterozygotes, the phenotype of WD to a small extent depends on the type of mutation coexisting with the p.H1069Q. Besides genotype, additional modifying factors seem to determine WD manifestations. Copyright (c) 2005 Movement Disorder Society.

  16. Analyzing the some biochemical parameters of diabetes mellitus and obese patients who applied to Siirt State Hospital endocrine polyclinic and their prevalence

    Science.gov (United States)

    Karageçili, Hasan; Yerlikaya, Emrah; Aydin, Ruken Zeynep

    2016-04-01

    Obesity and diabetes are major public health problems throughout the World. Obese individuals body mass index (BMI) is >30 kg/m2. Obesity is characterized by increased waist circumference, total body fat and hyperglycemia. The increased triglyceride and cholesterol level is also shown in obese individuals. The development of obesity is largely due to the consumption of high energy food and sedentary lifestyle. This study was held with the participation of patients applied to Siirt State Hospital endocrine policlinic for treatment. Our aim is to try to determine the biochemical relation and border line of obese and obese+diabetes mellitus patients. Patients and control group lipid profiles were studied in the hospital biochemisty laboratory. Laboratory results of diabetes+obese, obese and control groups were evaluated. Patients and control samples blood serum levels were compared according to their lipid profiles. In 2015, 735 diabetes mellitus type 2 patients applied to Endocrine polyclinic. Some of these patient's serum levels were evaluated. Difference between diabetes+obese and diabetes groups were near critical level for LDL and trigliserid. There were not observed statistically significant difference between groups in terms of HDL and cholesterol. There were found significant difference between groups for blood glucose p<0.003, age p<0.001. According to gender between women and men serum levels, ALT and AST levels; p<0.006 and cholesterol; p<0.04 were detected. According to participants education level blood biochemistry levels were observed statisticaly different p<0.001 with non-literacy group. In conclusion, obese and obese+diabetes patients blood serum values nearly close to each other. Obese subjects were been diabetic obese with age. In women obesity and diabetes mellitus prevalence were seen too much.

  17. A cross-sectional study of inpatients with late stage of dementia in Southeast China and the associations between biochemical parameters and apolipoprotein E genotypes

    Directory of Open Access Journals (Sweden)

    Jiang B

    2018-01-01

    Full Text Available Bin Jiang,1,2,* Xue-Ping Zhang,3,* Mei-Fang Chen,3 Zheng Wang,3 Ming-Xue Zhao,3 Bin-Hua Chen,3 Hong-Lei Li1 1Department of Neurology and Research Center of Neurology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 2Department of Neurology, The First Affiliated Hospital of Xiamen University, Xiamen, 3The Seventh People’s Hospital of Hangzhou, Mental Health Center of Zhejiang University School of Medicine, Hangzhou, People’s Republic of China *These authors contributed equally to this work Background: The purpose of this study was to analyze the disease distribution of patients in the late stage of dementia through a cross-sectional investigation and to clarify the association between apolipoprotein E (APOE genotypes and the serum levels of total cholesterol, total triglycerides, and blood glucose in the late-stage Alzheimer’s disease (AD patients.Methods: Patients who were in the late stage of dementia in a mental health center were enrolled in this study. A broad battery of neuropsychological tests and neuroimaging was applied to make the diagnosis. The APOE genotype was determined by the multiplex amplification refractory mutation system polymerase chain reaction. The association between APOE genotype and the blood level of biochemical parameters was studied.Results: A total of 155 patients were enrolled in this study. The majority of patients had AD (67.8%, followed by vascular dementia (15.5%, mixed dementia (9%, and others (7.7%. The APOE ε4 allele frequency was significantly different in the different groups. The serum level of total cholesterol (TC in APOE ε4 carriers was higher than in non-carriers (P<0.05. No statistically significant differences were found in the blood glucose and triglycerides (TG levels between these two groups.Conclusion: To our knowledge, this is the first paper to study the characteristics of late-stage dementia in hospital patients in Southeast China. We found that the

  18. The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats

    Directory of Open Access Journals (Sweden)

    Micovic Zarko

    2016-09-01

    Full Text Available Hyperhomocysteinemia (HHC, both basal and after methionine load, may occur due to genetic disorders or deficiencies of nutrients that affect the remethylation or trans-sulphuration pathways during methionine metabolism. HHC is involved in the pathogenesis of many illnesses as a result of its prooxidative effect and its impairment of antioxidative protection. The aim was to examine the effects of subchronic methionine overload on the body weight and standard biochemical parameters in rat serum and to examine whether simultaneous subchronic intraperotoneal administration of methionine alone or together with L-cysteine or N-acetyl-cysteine resulted in a change in the body weight and biochemical parameters in the rat serum. The research was conducted during a three-week period (male Wistar albino rats, n=36, body weight of approximately 160 g, age of 15-20 days, and the animals were divided into a control group and three experimental groups of 8-10 animals each: a control group (0.9% sodium chloride 0.1-0.2 ml/day; b methionine (0.8 mmol/kg/bw/day (MET group; c methionine (0.8 mmol/kg/bw/day + L-cysteine (7 mg/kg/bw/day (L-cys+MET group; and d methionine (0.8 mmol/kg/bw/day + N-acetyl-L-cysteine (50 mg/kg/bw/day (NAC+MET group. In addition to the body weight monitoring, the levels of total homocysteine and the standard biochemical parameters in blood samples (plasma or serum were determined. The results indicated that monitoring the homocysteine levels and standard biochemical parameters in blood could be used for analysis and could provide an excellent guideline for distinguishing between toxic and non-toxic doses of methionine intake, which may be meaningful for clinical applications.

  19. Comparing Effects Of GAMMA And Electron Beam Irradiation On Microbiological And Biochemical Characters Of Selected Egyptian Aromatic Herbs

    International Nuclear Information System (INIS)

    FARAG, S.A.; YUSSEF, B.M.; SHAMS EL DIEEN, N.M.; ABDALLA, M.

    2009-01-01

    Selected Egyptian aromatic herbs (AH) which face demand for exporting abroad, such as spearmint (Mentha viridis Linn.), rosemary (Rosmarinus officinalis L.) and marjoram (Origanum majorana L.), were exposed to gamma and electron beam (EB) irradiation at different doses (5 and 10 kGy). The evaluation of irradiation efficiency was based on microbiological and biochemical studies including changes in volatile compounds and antioxidative activity. The microbiological assay revealed presence of high load levels in raw AH with log of total bacteria of rosemary (6.862 cfu.g-1) and spearmint (6.762 cfu.g -1 ) while marjoram was the lowest one (5.771 cfu.g -1 ). The highest levels of the log of total molds and yeast counts were observed in marjoram (5.097 cfu.g -1 ) while the less levels were observed in rosemary and spearmint. Using gamma irradiation was more effective than EB in decontamination of microbes either in reducing numbers to safe levels at 5 kGy or completely elimination at high dose (10 kGy) in all tested samples. Some specific bacteria were present in AH such as Enterococcus faecalis, Bacillus cereus and Clostridium perfringens at different levels whereas the log of E. faecalis was detected only in marjoram and spearmint with heavy load as 4.554 and 3.427 cfu.g -1 , respectively, while rosemary was less than 2 cfu.g -1 . Irradiation with both sources at 5 kGy reduced the count by about 2 log cycles in marjoram and 1 log cycle in spearmint. No colonies of the same microbe were detected at high dose (10 kGy) in all herbs with exception of marjoram. The log of B. cereus contamination was higher in spearmint than marjoram then rosemary. Irradiation of AH at 5 kGy reduced these values whereas high dose (10 kGy) caused complete elimination of B. cereus.GLC analysis of AH volatile oils showed presence of 22 compounds in spearmint included mainly carvon specific smells of spearmint besides monoterpens as α- -pinen (1.6%), Β-pinen (2.06%), limonene (4.75%). Also

  20. COMPARING INTRA- AND INTERENVIRONMENTAL PARAMETERS OF OPTIMAL SETTING IN BREEDING EXPERIMENTS

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    Domagoj Šimić

    2004-06-01

    Full Text Available A series of biometrical and quantitative-genetic parameters, not well known in Croatia, are being used for the most important agronomic traits to determine optimal genotype setting within a location as well as among locations. Objectives of the study are to estimate and to compare 1 parameters of intra-environment setting (effective mean square error EMSE, in lattice design, relative efficiency RE, of lattice design LD, compared to randomized complete block design RCBD, and repeatability Rep, of a plot value, and 2 operative heritability h2, as a parameter of inter-environment setting in an experiment with 72 maize hybrids. Trials were set up at four environments (two locations in two years evaluating grain yield and stalk rot. EMSE values corresponded across environments for both traits, while the estimations for RE of LD varied inconsistently over environments and traits. Rep estimates were more different over environments than traits. Rep values did not correspond with h2 estimates: Rep estimates for stalk rot were higher than those for grain yield, while h2 for grain yield was higher than for stalk rot in all instances. Our results suggest that due to importance of genotype × environment interaction, there is a need for multienvironment trials for both traits. If the experiment framework should be reduced due to economic or other reasons, decreasing number of locations in a year rather than decreasing number of years of investigation is recommended.

  1. POLYMORPHISM AT THE ADH AND ALPHA-GPDH LOCI IN DROSOPHILA-MELANOGASTER - EFFECTS OF REARING TEMPERATURE ON DEVELOPMENTAL RATE, BODY-WEIGHT, AND SOME BIOCHEMICAL PARAMETERS

    NARCIS (Netherlands)

    OUDMAN, L; VANDELDEN, W; KAMPING, A; BIJLSMA, R

    The role of developmental time in the world-wide cline of Adh and alpha-Gpdh allele frequencies of Drosophila melanogaster, and the relationship with weight and some biochemical characters, were investigated. Experimental strains were constructed with different combinations of Adh and alpha-Gpdh

  2. A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of unprocessed Angelica sinensis and its 4 kinds of processed products.

    Science.gov (United States)

    Ji, Peng; Wei, Yanming; Hua, Yongli; Zhang, Xiaosong; Yao, Wanling; Ma, Qi; Yuan, Ziwen; Wen, Yanqiao; Yang, Chaoxue

    2018-01-30

    Angelica sinensis (AS), root of Angelica sinensis (Oliv.) Diels, an important kind of Chinese traditional herbal medicine, has been used for women to enrich the blood for thousands of years. It is mainly distributed in Gansu province of China. According to Traditional Chinese medicine usage, unprocessed AS (UAS) and its 4 kinds of processed products (ASs) are all used to treat different diseases or syndromes. The difference among the enriching-blood effects of ASs is unclear. And their exact mechanisms of enriching the blood are not fully understood. In this study, our aim is to compare the enriching-blood effect and explain the related mechanism of ASs, to lay the foundation for the blood deficiency diagnosis and the rational use of ASs in the clinic. ASs were used to intervene the blood deficiency syndrome model mice induced by acetyl phenylhydrazine (APH) and cyclophosphamide (CTX). A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of ASs was established. The blood routine examination, ATPase, glucose-6-phosphate dehydrogenase, methemoglobin, glutathion peroxidase, glutathione reductase, and erythropoietin were measured. Two biofluids (plasma and urine) obtained from mice were analyzed with GC-MS. Distinct changes in metabolite patterns of the two biofluids after mice were induced by APH and CTX, and mice were intervened with ASs were analyzed using partial least squares-discriminant analysis. Potential biomarkers were found using a novel method including variable importance in the projection (VIP) >1.0, volcano plot analysis, and significance analysis of microarray. The results of hematological, biochemical parameters and the integrated metabolomics all showed the blood deficiency syndrome model was built successfully, ASs exhibited different degree of enriching-blood effect, and AS pached with alcohol (AAS) exhibited the best enriching-blood effect. 16 metabolites in

  3. Comparative Biochemical and Functional Studies on a Branded Human Recombinant Factor VIIa and a Biosimilar Equivalent Product.

    Science.gov (United States)

    Sadeghi, Nasiredin; Kahn, Daniel; Syed, Daneyal; Iqbal, Omer; Abro, Schuharazad; Eshraghi, Reza; Hoppensteadt, Debra; Fareed, Jawed

    2014-09-01

    Recombinant factor VIIa (rFVIIa; NovoSeven, Novo Nordisk, Copenhagen, Denmark) is used to control bleeding in patients with hemophilia. A generic version of FVIIa was developed by AryoGen (Tehran, Iran). This study compared the composition and functional activities of AryoSeven and NovoSeven. Each product was compared at equigravimetric (1 mg/mL) stock solution for protein content. The proteomic profile was obtained using surface-enhanced laser desorption ionization mass spectrometry. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis was carried out to determine the protein profile and Western blotting was performed using a polyclonal rabbit antihuman FVIIa antibody. The FVIIa-related antigen was also measured using a commercially available enzyme-linked immunosorbent assay method. Functional assay included the prothrombin time correction in FVII-deficient plasma. The protein content was comparable in 2 products and the mass spectra analysis showed a single peak at 50 kDa in all products. The SDS-PAGE and immunoblotting studies were comparable. Both products exhibited similar coagulant properties in different assays. © The Author(s) 2014.

  4. Efficacy of topical honey therapy against silver sulphadiazine treatment in burns: A biochemical study

    OpenAIRE

    Nagane, N. S.; Ganu, J. V.; Bhagwat, V. R.; Subramanium, M.

    2004-01-01

    Thermal injury is associated with biochemical changes. The present study was undertaken to investigate relation of oxidative free radical generation and related biochemical parameters in burn trauma. The specific aim was to compare the levels of serum lipid peroxide, Ceruloplasmin and Uric Acid in burn patients during treatment with Silver Sulfadiazine Cream and honey therapy. It is a single blind prospective controlled study involving comparison of biochemical changes after treatment with si...

  5. Transcranial magnetic stimulation (TMS): compared sensitivity of different motor response parameters in ALS.

    Science.gov (United States)

    Pouget, J; Trefouret, S; Attarian, S

    2000-06-01

    Owing to the low sensitivity of clinical signs in assessing upper motor neuron (UMN) involvement in ALS, there is a need for investigative tools capable of detecting abnormal function of the pyramidal tract. Transcranial magnetic stimulation (TMS) may contribute to the diagnosis by reflecting a UMN dysfunction that is not clinically detectable. Several parameters for the motor responses to TMS can be evaluated with different levels of significance in healthy subjects compared with ALS patients. The central motor conduction time, however, is not sensitive in detecting subclinical UMN defects in individual ALS patients. The amplitude of the motor evoked potential (MEP), expressed as the percentage of the maximum wave, also has a low sensitivity. In some cases, the corticomotor threshold is decreased early in the disease course as a result of corticomotor neuron hyperexcitability induced by glutamate. Later, the threshold increases, indicating a loss of UMN. In our experience, a decreased silent period duration appears to be the most sensitive parameter when using motor TMS in ALS. TMS is also a sensitive technique for investigating the corticobulbar tract, which is difficult to study by other methods. TMS is a widely available, painless and safe technique with a good sensitivity that can visualize both corticospinal and corticobulbar tract abnormalities. The sensitivity can be improved further by taking into account the several MEP parameters, including latency and cortical silent period decreased duration.

  6. Comparative analysis of partial imaging performance parameters of home and imported X-ray machines

    International Nuclear Information System (INIS)

    Cao Yunxi; Wang Xianyun; Liu Huiqin; Guo Yongxin

    2002-01-01

    Objective: To compare and analyze the performance indexes and the imaging quality of the home and imported X-ray machines through testing their partial imaging performance parameters. Methods: By separate sampling from 10 home and 10 imported X-ray machines, the parameters including tube current, time of exposure, machine total exposure, and repeatability were tested, and the imaging performance was evaluated according to the national standard. Results: All the performance indexes met the standard of GB4505-84. The first sampling tests showed the maximum changing coefficient of imaging performance repeatability of the home X-ray machines was Δmax1 = 0.025, while that of the imported X-ray machine was Δmax1 = 0.016. In the second sampling tests, the maximum changing coefficients of the two were Δmax2 = 0.048 and Δmax2 = 0.022, respectively. Conclusion: The 2 years' follow-up tests indicate that there is no significant difference between the above-mentioned parameters of the elaborately adjusted home X-ray machines and imported ones, but the home X-ray machines are no better than the imported X-ray machines in stability and consistency

  7. Comparing UV/EUV line parameters and magnetic field in a quiescent prominence with tornadoes

    Science.gov (United States)

    Levens, P. J.; Labrosse, N.; Schmieder, B.; López Ariste, A.; Fletcher, L.

    2017-10-01

    Context. Understanding the relationship between plasma and the magnetic field is important for describing and explaining the observed dynamics of solar prominences. Aims: We determine if a close relationship can be found between plasma and magnetic field parameters, measured at high resolution in a well-observed prominence. Methods: A prominence observed on 15 July 2014 by the Interface Region Imaging Spectrograph (IRIS), Hinode, the Solar Dynamics Observatory (SDO), and the Télescope Héliographique pour l'Étude du Magnétisme et des Instabilités Solaires (THEMIS) is selected. We perform a robust co-alignment of data sets using a 2D cross-correlation technique. Magnetic field parameters are derived from spectropolarimetric measurements of the He I D3 line from THEMIS. Line ratios and line-of-sight velocities from the Mg II h and k lines observed by IRIS are compared with magnetic field strength, inclination, and azimuth. Electron densities are calculated using Fe xii line ratios from the Hinode Extreme-ultraviolet Imaging Spectrometer, which are compared to THEMIS and IRIS data. Results: We find Mg II k/h ratios of around 1.4 everywhere, similar to values found previously in prominences. Also, the magnetic field is strongest ( 30 G) and predominantly horizontal in the tornado-like legs of the prominence. The k3 Doppler shift is found to be between ±10 km s-1 everywhere. Electron densities at a temperature of 1.5 × 106 K are found to be around 109 cm-3. No significant correlations are found between the magnetic field parameters and any of the other plasma parameters inferred from spectroscopy, which may be explained by the large differences in the temperatures of the lines used in this study. Conclusions: This is the first time that a detailed statistical study of plasma and magnetic field parameters has been performed at high spatial resolution in a prominence. Our results provide important constraints on future models of the plasma and magnetic field in

  8. Comparative effect of clopidogrel plus aspirin and aspirin monotherapy on hematological parameters using propensity score matching

    Directory of Open Access Journals (Sweden)

    Hayasaka M

    2013-02-01

    Full Text Available Masatoshi Hayasaka,1 Yasuo Takahashi,2 Yayoi Nishida,2 Yoshikazu Yoshida,1 Shinji Hidaka,3 Satoshi Asai41Department of Pharmacy, Nihon University Itabashi Hospital, Tokyo, 2Division of Genomic Epidemiology and Clinical Trials, Clinical Trials Research Center, Nihon University School of Medicine, Tokyo, 3Laboratory of Pharmaceutical Regulatory Science, Department of Pharmacy, School of Pharmacy, Nihon University, Chiba, 4Division of Pharmacology, Department of Biomedical Sciences, Nihon University School of Medicine, Tokyo, JapanBackground: Clopidogrel and aspirin are antiplatelet agents that are recommended to reduce the risk of recurrent stroke and other cardiovascular events. Dual antiplatelet therapy with clopidogrel and aspirin has been shown to increase the risk of hemorrhage, but the effects of the drugs on laboratory parameters have not been well studied in real-world clinical settings. Therefore, we evaluated and compared the effects of combination therapy with clopidogrel plus aspirin and aspirin monotherapy on laboratory parameters.Methods: We used data from the Nihon University School of Medicine Clinical Data Warehouse obtained between November 2004 and May 2011 to identify cohorts of new users (n = 130 of clopidogrel (75 mg/day plus aspirin (100 mg/day and a propensity score matched sample of new users (n = 130 of aspirin alone (100 mg/day. We used a multivariate regression model to compare serum levels of creatinine, aspartate aminotransferase, and alanine aminotransferase, as well as hematological parameters including hemoglobin level, hematocrit, and white blood cell, red blood cell, and platelet counts up to 2 months after the start of administration of the study drugs.Results: There were no significant differences for any characteristics and baseline laboratory parameters between users of clopidogrel plus aspirin and users of aspirin alone. Reductions in white blood cell and red blood cell counts, hemoglobin levels, and

  9. Comparative Evaluation of Biochemical Changes in Tomato (Lycopersicon esculentum Mill.) Infected by Alternaria alternata and Its Toxic Metabolites (TeA, AOH, and AME).

    Science.gov (United States)

    Meena, Mukesh; Zehra, Andleeb; Dubey, Manish K; Aamir, Mohd; Gupta, Vijai K; Upadhyay, Ram S

    2016-01-01

    In the present study, we have evaluated the comparative biochemical defense response generated against Alternaria alternata and its purified toxins viz. alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA). The necrotic lesions developed due to treatment with toxins were almost similar as those produced by the pathogen, indicating the crucial role of these toxins in plant pathogenesis. An oxidative burst reaction characterized by the rapid and transient production of a large amount of reactive oxygen species (ROS) occurs following the pathogen infection/toxin exposure. The maximum concentration of hydrogen peroxide (H 2 O 2 ) produced was reported in the pathogen infected samples (22.2-fold) at 24 h post inoculation followed by TeA (18.2-fold), AOH (15.9-fold), and AME (14.1-fold) in treated tissues. 3,3'- Diaminobenzidine staining predicted the possible sites of H 2 O 2 accumulation while the extent of cell death was measured by Evans blue dye. The extent of lipid peroxidation and malondialdehyde (MDA) content was higher (15.8-fold) at 48 h in the sample of inoculated leaves of the pathogen when compared to control. The cellular damages were observed as increased MDA content and reduced chlorophyll. The activities of antioxidative defense enzymes increased in both the pathogen infected as well as toxin treated samples. Superoxide dismutase (SOD) activity was 5.9-fold higher at 24 h post inoculation in leaves followed by TeA (5.0-fold), AOH (4.1-fold) and AME (2.3-fold) treated leaves than control. Catalase (CAT) activity was found to be increased upto 48 h post inoculation and maximum in the pathogen challenged samples followed by other toxins. The native PAGE results showed the variations in the intensities of isozyme (SOD and CAT) bands in the pathogen infected and toxin treated samples. Ascorbate peroxidase (APx) and glutathione reductase (GR) activities followed the similar trend to scavenge the excess H 2 O 2 . The reduction in CAT

  10. Effect of Short-Term High Fat Diet Inducing Obesity on Hematological, Some Biochemical Parameters and Testicular Oxidative Stress in Male Rats

    Directory of Open Access Journals (Sweden)

    Sherif M. Shawky

    2015-10-01

    Full Text Available Obesity constitutes a health problem due to its increasing worldwide prevalence. Among the health detriments caused by obesity, reproduction is disrupted. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. The objective of the current work was to examine the effect of diet-induced obesity on blood parameters, liver and kidney function tests, lipid profile and testicular oxidative stress. For that purpose, Male rats were fed ad libitum with a standard diet (control group; n.= 15 and high fat diet (HFD group; n.= 15 for 6 weeks. Hematological parameters, urea, creatinine, albumin were similar between the two groups. Intergroup testosterone levels were also comparable. The high fat diet induced significant increase in serum triglycerides, cholesterol, low density lipoprotein and very low density lipoprotein cholesterol concentrations. This diet also increases significantly alanine aminotransferase and aspartate aminotransferase activities and decreased total protein level and high-density lipoprotein cholesterol concentration. Furthermore, HFD showed a significant increasing in malondialdehyde contents in testes and decreasing in superoxide dismutase activity, the results of this study concluded that short-term high fat diet affect on liver enzymes and causing oxidative stress in testes.

  11. Physical parameter determinations of young Ms. Taking advantage of the Virtual Observatory to compare methodologies

    Science.gov (United States)

    Bayo, A.; Rodrigo, C.; Barrado, D.; Allard, F.

    One of the very first steps astronomers working in stellar physics perform to advance in their studies, is to determine the most common/relevant physical parameters of the objects of study (effective temperature, bolometric luminosity, surface gravity, etc.). Different methodologies exist depending on the nature of the data, intrinsic properties of the objects, etc. One common approach is to compare the observational data with theoretical models passed through some simulator that will leave in the synthetic data the same imprint than the observational data carries, and see what set of parameters reproduce the observations best. Even in this case, depending on the kind of data the astronomer has, the methodology changes slightly. After parameters are published, the community tend to quote, praise and criticize them, sometimes paying little attention on whether the possible discrepancies come from the theoretical models, the data themselves or just the methodology used in the analysis. In this work we perform the simple, yet interesting, exercise of comparing the effective temperatures obtained via SED and more detailed spectral fittings (to the same grid of models), of a sample of well known and characterized young M-type objects members to different star forming regions and show how differences in temperature of up to 350 K can be expected just from the difference in methodology/data used. On the other hand we show how these differences are smaller for colder objects even when the complexity of the fit increases like for example introducing differential extinction. To perform this exercise we benefit greatly from the framework offered by the Virtual Observaotry.

  12. A comparative evaluation of semen parameters in pre- and post-Hurricane Katrina human population

    Directory of Open Access Journals (Sweden)

    Caner Baran

    2015-01-01

    Full Text Available A natural disaster leading to accumulation of environmental contaminants may have substantial effects on the male reproductive system. Our aim was to compare and assess semen parameters in a normospermic population residing in the Southern Louisiana, USA area pre- and post-Hurricane Katrina. We retrospectively evaluated semen analyses data (n = 3452 of 1855 patients who attended the Tulane University Andrology/Fertility Clinic between 1999 and 2013. The study inclusion criteria were men whose semen analyses showed ≥ 1.5 ml volume; ≥15 million ml -1 sperm concentration; ≥39 million total sperm count; ≥40% motility; >30% morphology, with an abstinence interval of 2-7 days. After the inclusion criteria applied to the population, 367 normospermic patients were included in the study. Descriptive statistics and group-based analyses were performed to interpret the differences between the pre-Katrina (Group 1, 1999-2005 and the post-Katrina (Group 2, 2006-2013 populations. There were significant differences in motility, morphology, number of white blood cell, immature germ cell count, pH and presence of sperm agglutination, but surprisingly there were no significant differences in sperm count between the two populations. This long-term comparative analysis further documents that a major natural disaster with its accompanied environmental issues can influence certain semen parameters (e.g., motility and morphology and, by extension, fertility potential of the population of such areas.

  13. Are Mussels Always the Best Bioindicators? Comparative Study on Biochemical Responses of Three Marine Invertebrate Species to Chronic Port Pollution.

    Science.gov (United States)

    Laitano, María V; Fernández-Gimenez, Analía V

    2016-07-01

    Bivalves have traditionally been considered good bioindicators due to their sensitivity to pollution, among other features. This characteristic is shared by several other non-bivalve species as well, though studies in this respect remain scarce. This work aims to compare biomarker sensitivity to chronic port pollution among three intertidal invertebrate species with good bioindicator characteristics. Mussels' immunological (phenoloxidase and peroxidases) and biotransformation (glutathione-S-transferase) responses were contrasted against those of limpets and barnacles. The three species under study evidenced activity of all the enzymes measured, although with differences. Barnacle Balanus glandula was the most sensitive species showing pollution modulation of the three enzymes, which suggests that mussels would not always be the best bioindicator species among marine invertebrates depending on the responses that are assessed.

  14. Ethanol extract of mango (Mangifera indica L.) peel inhibits α-amylase and α-glucosidase activities, and ameliorates diabetes related biochemical parameters in streptozotocin (STZ)-induced diabetic rats.

    Science.gov (United States)

    Gondi, Mahendranath; Prasada Rao, U J S

    2015-12-01

    Peel is a major by-product during processing of mango fruit into pulp. Recent report indicates that the whole peel powder ameliorated diabetes. In the present study, ethanolic extract of mango peel was analysed for its bioactive compounds, evaluated for α-amylase and α-glucosidase inhibitory properties, oral glucose tolerance test, antioxidant properties, plasma insulin level and biochemical parameters related to diabetes. In addition to gallic and protocatechuic acids, the extract also had chlorogenic and ferulic acids, which were not reported earlier in mango peel extracts. The peel extract inhibited α-amylase and α-glucosidase activities, with IC50 values of 4.0 and 3.5 μg/ml. Ethanolic extract of peel showed better glucose utilization in oral glucose tolerance test. Treatment of streptozotocin-induced diabetic rats with the extract decreased fasting blood glucose, fructosamine and glycated hemoglobin levels, and increased plasma insulin level. Peel extract treatment decreased malondialdehyde level, but increased the activities of antioxidant enzymes significantly in liver and kidney compared to diabetic rats. These beneficial effects were comparable to metformin, but better than gallic acid treated diabetic rats. The beneficial effects of peel extract may be through different mechanism like increased plasma insulin levels, decreased oxidative stress and inhibition of carbohydrate hydrolyzing enzyme activities by its bioactive compounds. Thus, results suggest that the peel extract can be a potential source of nutraceutical or can be used in functional foods and this is the first report on antidiabetic properties of mango peel extract.

  15. A Comparative biochemical study on two marine endophytes, Bacterium SRCnm and Bacillus sp. JS, Isolated from red sea algae.

    Science.gov (United States)

    Ahmed, Eman Fadl; Hassan, Hossam Mokhtar; Rateb, Mostafa Ezzat; Abdel-Wahab, Noha; Sameer, Somayah; Aly Taie, Hanan Anwar; Abdel-Hameed, Mohammed Sayed; Hammouda, Ola

    2016-01-01

    Two marine endophytic bacteria were isolated from the Red Sea algae; a red alga; Acanthophora dendroides and the brown alga Sargassum sabrepandum. The isolates were identified based on their 16SrRNA sequences as Bacterium SRCnm and Bacillus sp. JS. The objective of this study was to investigate the potential anti-microbial and antioxidant activities of the extracts of the isolated bacteria grown in different nutrient conditions. Compared to amoxicillin (25μg/disk) and erythromycin (15μg/disk), the extracts of Bacterium SRCn min media II, III, IV and V were potent inhibitors of the gram-positive bacterium Sarcina maxima even at low concentrations. Also, the multidrug resistant Staphylococcus aureus(MRSA) was more sensitive to the metabolites produced in medium (II) of the same endophyte than erythromycin (15μg/disk). A moderate activity of the Bacillus sp. JS extracts of media I and II was obtained against the same pathogen. The total compounds (500ug/ml) of both isolated endophytes showed moderate antioxidant activities (48.9% and 46.1%, respectively). LC/MS analysis of the bacterial extracts was carried out to investigate the likely natural products produced. Cyclo(D-cis-Hyp-L-Leu), dihydrosphingosine and 2-Amino-1,3-hexadecanediol were identified in the fermentation medium of Bacterium SRCnm, whereas cyclo (D-Pro-L-Tyr) and cyclo (L-Leu-L-Pro) were the suggested compounds of Bacillus sp. JS.

  16. A comparative study on the adverse effects of flunixin, ketoprofen and phenylbutazone in miniature donkeys: haematological, biochemical and pathological findings.

    Science.gov (United States)

    Mozaffari, A A; Derakhshanfar, A; Alinejad, A; Morovati, M

    2010-10-01

    To evaluate the adverse effects of flunixin, ketoprofen and phenylbutazone when administered I/V to clinically normal miniature donkeys. Twenty clinically normal adult (2.0-2.5 years old) male miniature donkeys weighing 113-136 kg and 0.81- 0.86 m tall were randomly assigned to one of four groups, and administered either saline (n=5), 1.0 mg/kg flunixin (n=5), 2.2 mg/kg ketoprofen (n=5), or 4.4 mg/kg phenylbutazone (n=5) I/V at 0800 hours on Day 1, then every 12 h, for 12 days. The animals were observed every 8 h, and examined physically daily. Blood, faeces and urine samples were collected daily from all donkeys, for haematological indices and enzyme activities, occult blood, and urinalysis, respectively. Immediately after euthanasia, complete post-mortem examinations were performed on all donkeys, and gross lesions recorded. Histopathology was conducted on a wide range of tissues. Clinically, mild anorexia and diarrhoea were observed during the study only in donkeys treated with phenylbutazone. There was an effect of treatment with the non-steroidal anti-infl ammatory drugs (NSAID) on red blood cell (RBC) counts, packed cell volume (PCV) and enzyme activities, but not on urine. Lesions were observed in the glandular mucosa of the stomach of all donkeys treated with NSAID, including ulceration in most. Also, in donkeys treated with NSAID, hyperaemia, erosion and ulceration of the gastrointestinal tract, and congestion of the liver, kidney and spleen, were observed. Microscopically, hepatic and renal lesions comprised biliary hyperplasia and interstitial nephritis, respectively. The gastrointestinal, hepatic and renal lesions observed in the donkeys treated with NSAID demonstrated the toxic potential of NSAID, which was greatest for animals treated with phenylbutazone, less for flunixin, and least for ketoprofen. When use of these compounds is contemplated in clinical cases, the risk of adverse effects and the comparative toxic potential should be considered

  17. Variation of inflammatory parameters after sibutramine treatment compared to placebo in type 2 diabetic patients.

    Science.gov (United States)

    Derosa, G; Maffioli, P; Ferrari, I; Palumbo, I; Randazzo, S; D'Angelo, A; Cicero, A F G

    2011-10-01

    The efficacy of sibutramine has been demonstrated in randomized trials in obese/overweight patients including those with type 2 diabetes mellitus (T2DM). Our objective was to evaluate the effects of 1-year treatment with sibutramine compared to placebo on body weight, glycaemic control, lipid profile, and inflammatory parameters in type 2 diabetic patients. Two hundred and forty-six patients with uncontrolled T2DM [glycated haemoglobin (HbA(1c) ) > 8·0%] in therapy with different oral hypoglycaemic agents or insulin were randomized to take 10 mg of sibutramine or placebo for 12 months. We evaluated at baseline, and after 3, 6, 9, and 12 months these parameters: body weight, body mass index (BMI), HbA(1c) , fasting plasma glucose (FPG), post-prandial plasma glucose (PPG), fasting plasma insulin (FPI), homeostasis model assessment insulin resistance index (HOMA-IR), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C), triglycerides (Tg), leptin, tumour necrosis factor-α (TNF-α), adiponectin (ADN), vaspin, high sensitivity C-reactive protein (Hs-CRP). We observed a decrease of body weight after 9 and 12 months in the group treated with sibutramine, but not in the control group. Regarding glycaemic and lipid profile, although there are differences seen over time within each of the groups, we did not obtain any significant differences between the two groups. Both placebo and sibutramine gave a similar improvement of HOMA-IR, leptin, TNF-α, ADN, and Hs-CRP. No vaspin variations were observed in either group. Sibutramine resulted in a decrease in body weight at 9 months and at 12 months that was not observed with placebo. Although there were differences seen over time within each of the groups, there were no significant differences between groups for any other parameter that we measured. © 2010 The Authors. JCPT © 2010 Blackwell Publishing Ltd.

  18. Comparative Study on Quality Parameters of Royal Jelly, Apilarnil and Queen Bee Larvae Triturate

    Directory of Open Access Journals (Sweden)

    Rodica MARGAOAN

    2017-05-01

    Full Text Available Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans.Â

  19. Biochemical parameters of Spodoptera frugiperda (J. E. Smith) treated with citronella oil (Cymbopogon winterianus Jowitt ex Bor) and its influence on reproduction.

    Science.gov (United States)

    Silva, Cristiane Thalita Dos Santos; Wanderley-Teixeira, Valéria; Cunha, Franklin Magliano da; Oliveira, José Vargas de; Dutra, Kamilla de Andrade; Navarro, Daniela Maria do Amaral Ferraz; Teixeira, Álvaro Aguiar Coelho

    2016-05-01

    Spodoptera frugiperda is the principal corn pest in Brazil. Searches for new control methods that minimize the adverse effects of synthetic insecticides have initiated a resurgence of the use of botanical insecticides. Citronella oil (a product of Cymbopogon winterianus) is an effective repellent and insecticide. Thus, biochemical profile changes in oil-treated larvae and its influence on reproduction were assessed. Corn leaves dipped in a 50mg/mL concentration were offered to third instar larvae for 24h and assessed in sixth instar to estimate protein, lipid, sugar, and glycogen levels. Adult testes and ovarioles were collected for histological and histochemical analysis 24h after emergence. Number of eggs and hatching rate were also measured. Oil-treated larvae showed an increase in glycogen and a decrease in protein, lipid, and totals sugar content. Control testes exhibited connective tissue lining and cysts with abundant spermatozoids. However, intense peripheral vacuolation and neutral carbohydrates reduction occurred in oil-treated individuals. Control ovarioles showed normal morphologic characteristics. On the other hand, oil-treatment ovarioles showed follicular cell stratification and removal, reduced nurse cell development, reduced yolk quantity, a thinner conjunctiva sheath, and a reduction in proteins and neutral carbohydrates. Eggs derived from oil-treated pairs were unviable. Therefore, sub-lethal doses of citronella oil alters the biochemical profile of S. frugiperda larvae, causing damage to their reproductive histophysiology and results in diminished reproduction or reproductive failure. Copyright © 2016 Elsevier GmbH. All rights reserved.

  20. In-vivo study for anti-hyperglycemic potential of aqueous extract of Basil seeds (Ocimum basilicum Linn) and its influence on biochemical parameters, serum electrolytes and haematological indices.

    Science.gov (United States)

    Chaudhary, Sachin; Semwal, Amit; Kumar, Hitesh; Verma, Harish Chandra; Kumar, Amit

    2016-12-01

    The study introduced anti-hyperglycemic influence of aqueous extract of Ocimum basilicum seeds (AEOBS) in Streptozotocin (STZ) induced diabetic rats and estimating its potential to ameliorate altered level of biochemical parameters, serum electrolytes level and haematological indices along with its effect on body weight of treated rats. The albino rats were selected to observe oral glucose tolerance test by oral intake of aq. glucose solution (4g/kg, body weight) in normal rats and estimation of blood glucose level after administration of AEOBS at 250mg/kg, 500mg/kg and standard drug glibenclamide at 0.6mg/kg, body weight. Antidiabetic activity was evaluated in chronic study models by STZ induced diabetes in rats followed by blood glucose estimation. Chronic study model was selected to carry out further studies to evaluate the effect of AEOBS at 250mg/kg, 500mg/kg and standard drug on body weight, alterations in biochemical parameters including AST, ALT, ALP, total bilirubin and total protein, alterations in serum electrolytes like Na + , K + , Cl - , HCO 3 - along with estimation of haematological indices like red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), lymphocytes, neutrophils, eosinophils, monocytes and basophils. AEOBS significantly reduced the blood glucose level of diabetic rats at both doses. Body weight was also improved significantly. Similarly, the levels of biochemical parameters, serum electrolytes, and haematological indices were significantly ameliorated at both doses of AEOBS. The histopathological results revealed reconstitution of pancreatic islets towards normal cellular architecture in rats treated with AEOBS. The results illustrated that AEOBS have eminent antidiabetic potential in STZ effectuated diabetes in rats and can be extensively used for the treatment of diabetes mellitus-II and its associated complications including anaemia, diabetic nephropathy, liver dysfunction, and immunosuppression. Copyright © 2016 Elsevier

  1. Study of seasonal variation biochemical parameters of rodents and lagomorphs from the Vivarium of the Faculty of Medicine, University of São Paulo, São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Marilda Osti Spinelli

    2014-06-01

    Full Text Available ABSTRACT. Spinelli M.O., da Cruz R.J., de Godoy C.M.S.C., Motta M.C. & Damy S.B. [Study of seasonal variation biochemical parameters of rodents and lagomorphs from the Vivarium of the Faculty of Medicine, University of São Paulo, São Paulo, Brazil.] Estudo da variação sazonal dos parâmetros bioquímicos de roedores e lagomorfos do biotério da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brasil. Revista Brasileira de Medicina Veterinária, 36(2:219-225, 2014. Faculdade de Medicina, Divisão Técnica de Apoio ao Ensino e Pesquisa, Biotério Central, Avenida Doutor Arnaldo, 455, Cerqueira César, São Paulo, SP 01246-903, Brasil. E-mail: marilda@biot.fm.usp.br This work aimed to study the seasonal variation of the biochemical parameters of mice inbred BALB / c and C57BL / 6 mice, rats and rabbits New Zealand, the Vivarium of the Faculty of Medicine, University of Sao Paulo. Which is its clinical significance, assessing the analytes plasma ALT (alanine aminotransferase, AST (aspartate aminotransaminase, urea, urea-BUN (blood urea nitrogen, creatinine, total cholesterol and fractions VLDL (very low density lipoprotein, HDL (high density lipoprotein and LDL (low density lipoprotein? The results showed an increase in concentrations of analytes, statistical significance, especially in summer. The hypotheses that causes nutritional, infectious, water stress or climatic variations did not affect them, suggesting that to assess the health status of colonies of laboratory animals, biochemical parameters must be considered within the range of variation expected for each colony under study.

  2. Effects of Different Levels of Coriander (Coriandrum sativum Seed Powder and Extract on Serum Biochemical Parameters, Microbiota, and Immunity in Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Hesam Hosseinzadeh

    2014-01-01

    Full Text Available The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4 compared to T1 and T2. Furthermore, there were no treatment effects on Lactobacillus bacteria; however, the population of E. coli was significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2 and T3 at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.

  3. Effect of priming on growth, biochemical parameters and mineral composition of different cultivars of coriander (Coriandrum sativum L. under salt stress

    Directory of Open Access Journals (Sweden)

    Ben Fredj Meriem

    2014-08-01

    Full Text Available At Higher Institute of Agriculture of Chott Mariem, Sousse, Tunisia, this study was conducted to evaluate the interactive effect of salinity and seed priming on coriander. The experiment was carried out in completely randomized design with three replications consisting of four coriander genotypes (Tunisian cv, Algerian cv, Syrian cv and Egyptian cv at two seed conditions (seed priming with 4 g/l NaCl for 12h or no seed priming. Results revealed that seed priming and salinity had significantly (p≤0.05 affected all the parameters under study. On the first hand, salinity stress had adversely affected growth, chlorophyll content, mineral composition (K+ and Ca2+ of coriander in all genotypes. Also, it activated Na+ accumulation and synthesis of proline, soluble sugars and proteins. However, seed priming with NaCl had diminished the negative impact of salt stress in all cultivars and primed plants showed better response to salinity compared to unprimed plants. Maximum values were recorded in tolerant cultivar which is Tunisian one whereas minimum values were noted in sensitive cultivar (Algerian cv.

  4. Quantitative gated SPECT- a comparative study of two algorithms for parameters of perfusion and LV function

    International Nuclear Information System (INIS)

    Ali, A.Z.

    2007-01-01

    Full text: Aim: To compare the perfusion and LV function parameters as quantified by 4D-MSPECT and ECT in the same patient group and a qualitative comparison of the reconstructed slices by two different experts. Materials and methods: Thirty-one consecutive patients underwent gated myocardial perfusion SPECT using a two-day protocol. The gated and ungated data were reconstructed by back projection method. Quantitative analysis was performed on the same set of reconstructed slices by 4D-MSPECT and Emory Cardiac Tool Box. The reconstructed slices were read qualitatively by two different experts on their respective systems. Polar map and functional analysis was performed in both softwares and the results were compared. Results: The concordance between the two experts qualitatively was seen in 78/93(84%) coronary territories. The polar map defects were comparable in LAD (r-value of 0.87) and LCX (r-value of 0.76) territories whereas RCA defects (r-value of 0.04) were not at all correlating. The defect severity showed concordance in 68/93 (73%) coronary territories. There was concordance between 4DMSPECT and the qualitative interpretation in 84/93 (90%) coronary territories whereas ECT showed concordance in only 70/93(75%) coronary territories. The overall sensitivity is marginally higher for ECT (100% vs. 96%) but the overall specificity is much higher with 4 DMSPECT (88% vs. 65%). ESV showed good correlation(r=0.94) of the two softwares with no significant difference in means. EDV and LVEF although had good correlation(r = 0.96 and 0.89) showed high difference in means (p<0.01). Conclusion: Between 4D-MSPECT and ECT, 4D-MSPECT is marginally superior to ECT with reference to qualitative interpretation in view of better specificity. The LVEF values between the two softwares should also not be used interchangeably. (author)

  5. Probabilistic Analysis for Comparing Fatigue Data Based on Johnson-Weibull Parameters

    Science.gov (United States)

    Vlcek, Brian L.; Hendricks, Robert C.; Zaretsky, Erwin V.

    2013-01-01

    Leonard Johnson published a methodology for establishing the confidence that two populations of data are different. Johnson's methodology is dependent on limited combinations of test parameters (Weibull slope, mean life ratio, and degrees of freedom) and a set of complex mathematical equations. In this report, a simplified algebraic equation for confidence numbers is derived based on the original work of Johnson. The confidence numbers calculated with this equation are compared to those obtained graphically by Johnson. Using the ratios of mean life, the resultant values of confidence numbers at the 99 percent level deviate less than 1 percent from those of Johnson. At a 90 percent confidence level, the calculated values differ between +2 and 4 percent. The simplified equation is used to rank the experimental lives of three aluminum alloys (AL 2024, AL 6061, and AL 7075), each tested at three stress levels in rotating beam fatigue, analyzed using the Johnson- Weibull method, and compared to the ASTM Standard (E739 91) method of comparison. The ASTM Standard did not statistically distinguish between AL 6061 and AL 7075. However, it is possible to rank the fatigue lives of different materials with a reasonable degree of statistical certainty based on combined confidence numbers using the Johnson- Weibull analysis. AL 2024 was found to have the longest fatigue life, followed by AL 7075, and then AL 6061. The ASTM Standard and the Johnson-Weibull analysis result in the same stress-life exponent p for each of the three aluminum alloys at the median, or L(sub 50), lives

  6. Comparative binding characteristics of Tc-CPI, Tc-TBI, and Tc-MIBI in cultured heart cells: Correlation with biochemical analysis and animal images

    International Nuclear Information System (INIS)

    Piwnica-Worms, D.; Kronauge, J.F.; Holman, B.L.; Davison, A.; Jones, A.G.

    1987-01-01

    Hexakis (isonitrile)technetium (I) complexes are a new class of cationic, lipophilic myocaridal perfusion imaging agents. To better understand their cellular mechanisms of uptake and washout, chick heart cells grown in culture were used as a model myocardial system. Tc-MIBI showed uptake to a plateau at a rate similar to Tc-CPI (t1/2 = 4.1 +- 0.7 minutes); however, the plateau was 63% greater. Tc-TBI uptake approached a plateau 900% greater than Tc-CPI binding. Heart cell studies showed washout of Tc-CPI>Tc-TBI>Tc-MIBI, which correlated with kinetic analysis of rabbit myocardial images. Biochemical in vitro analysis in human plasma demonstrated 75% enzymatic ester hydrolysis of Tc-CPI by 3 minutes, but no hydrolysis of Tc-TBI and Tc-MIBI. The results suggest that metabolism of the ester function of Tc-CPI following myocardial uptake may in part account for the more rapid cellular washout rates of Tc-CPI compared with Tc-TBI and Tc-MIBI

  7. Effects of Moringa oleifera leaves as a substitute for alfalfa meal on nutrient digestibility, growth performance, carcass trait, meat quality, antioxidant capacity and biochemical parameters of rabbits.

    Science.gov (United States)

    Sun, B; Zhang, Y; Ding, M; Xi, Q; Liu, G; Li, Y; Liu, D; Chen, X

    2018-02-01

    This contribution reports the effects of Moringa oleifera leaves (MOLs) meal on the growth performances, nutrient digestibility, carcass trait, meat quality, antioxidant capacity and biochemical parameters of growing New Zealand white rabbits. The MOL was substituted for alfalfa meal at levels of 0, 10%, 20% and 30% to obtain respective diets MOL0, MOL10, MOL20 and MOL30. Each treatment was replicated five times with 10 rabbits per replicate. Results showed the average daily weight gain (ADWG) and feed conversion ratio (FCR) of rabbits fed MOL20 diet were significantly better (p oleifera leaves (MOL0, MOL10). The meat drip loss of rabbits fed with diet MOL10 was significantly lower (p oleifera leaves. No significant differences were found in the digestibility of crude fibre (CF), crude fat (EE), ash, crude protein (CP) and nitrogen-free extract (NFE) among the dietary groups. Moringa oleifera leaves also have a significant impact on serum albumin (ALB), low-density lipoprotein cholesterol (LDLC), triiodothyroxine (T 3 ) and tetraiodothyroxine (T 4 ) values and the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and liver. The results indicated that M. oleifera leaves could be developed as a good feed source, and it not only could substitute for alfalfa meal well but also has a significant effect on growth performance, meat quality, antioxidant and biochemical parameters of rabbits. © 2017 Blackwell Verlag GmbH.

  8. Investigating the effects of using Nettle (Urtica dioica , Menta pulagum (Oreganum valgare and Zizaphora (Thymyus valgaris medicinal plants on performance, carcass quality, blood biochemical parameters and blood cells of broilers

    Directory of Open Access Journals (Sweden)

    A Heydari

    2010-11-01

    Full Text Available This experiment was conducted to evaluate the effects of using Nettle,Menta pulagum and ‌‌Zizaphora medicinal plants on performance, carcass quality, blood biochemical parameters and blood cells of broilers. The experiment was conducted in a completely randomized design with 288 broilers (Ross-308 in 8 treatments and 3 replicates (with 12 birds in each replicate from 1 to 42 days and included: 1 control group without using any medicinal plants, 2 1.5% of ‌‌Nettle, 3 1.5% of Menta pulagum, 4 1.5% of Zizaphora, 5 1.5% of Nettle and Menta pulagum, 6 1.5% of  Nettle and Zizaphora, 7 1.5% of Menta pulagum and ‌‌Zizaphora, 8 1.5% of Nettle,Menta pulagum and ‌‌Zizaphora. The results showed that using these medicinal plants and their mixtures had significant effects on performance, carcass traits and blood biochemical parameters of broilers (p

  9. Clinico-haematological and serum biochemical alterations in ...

    African Journals Online (AJOL)

    An increase in serum CRE and BUN values were recorded in all cases of pyometra which reduced to lower levels during both treatments in follow-ups. All the haemato-biochemical parameters were comparable to their respective reference values after either medicinal treatment or ovariohysterectomy of dogs. Thus the dogs ...

  10. Identification of critical parameters for PEMFC stack performance characterization and control strategies for reliable and comparable stack benchmarking

    DEFF Research Database (Denmark)

    Mitzel, Jens; Gülzow, Erich; Kabza, Alexander

    2016-01-01

    This paper is focused on the identification of critical parameters and on the development of reliable methodologies to achieve comparable benchmark results. Possibilities for control sensor positioning and for parameter variation in sensitivity tests are discussed and recommended options for the ...

  11. Investigating and comparing uranium and gamma radiation induced effects on photosynthetic parameters for Arabidopsis thaliana

    Energy Technology Data Exchange (ETDEWEB)

    Vanhoudt, Nathalie; Horemans, Nele; Saenen, Eline; Biermans, Geert; Nauts, Robin; Wannijn, Jean; Van Hees, May; Vandenhove, Hildegarde [Belgian Nuclear Research Centre (SCK.CEN), Biosphere Impact Studies, 2400, Mol (Belgium)

    2014-07-01

    As the environment is inevitably exposed to radionuclides and ionizing radiation from natural and anthropogenic sources, it is important to study the effects induced by these stressors on plants. In addition, it is already known that photosynthesis can be affected under various metal exposure situations. The objective of this research is to compare uranium induced effects with gamma radiation induced effects on photosynthetic parameters in Arabidopsis thaliana. First, 18-day-old seedlings were exposed to 50 μM uranium during 4 days. Second, 14-day-old seedlings were exposed to gamma radiation for 7 days to a total dose of 6.7 Gy. By using chlorophyll fluorescence measurements, the photosynthetic performance was assessed. Based on the data obtained during the measurement of induction curves, parameters providing information on the photosynthetic efficiency and heat dissipation can be calculated. For uranium exposed leaves, it was observed that the potential photosynthetic efficiency (measured as Fv/Fm) remained maximal while the effective efficiency of photosystem II (φPSII), which is a measure for the proportion of light absorbed by PSII used in photochemistry, even increased. The increase of φPSII could be related to a decrease in non-photochemical quenching (NPQ), which reflects the protective mechanism against excess light intensity by converting energy into heat, but no alterations in non-regulated energy dissipation (NO). A high NO value would indicate the inefficiency of photochemistry and heat conversion and the plant's inability to regulate the radiation energy. In plants exposed to uranium, NO levels were similar to the control. Under gamma irradiation, the capacity of PSII remained intact and plants started optimizing their photosynthetic process by increasing φPSII and decreasing NPQ. When comparing the NPQ kinetic responses of gamma radiation and uranium exposure, a remarkable difference can be highlighted. While gamma radiation exposure

  12. Effect of Calcium Soap of Fatty Acids Supplementation on Serum Biochemical Parameters and Ovarian Activity during Out-of-the-Breeding Season in Crossbred Ewes

    Science.gov (United States)

    El-Nour, Hayat H. M.; Nasr, Soad M.; Hassan, Walid R.

    2012-01-01

    This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA) supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45–55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (P < 0.05) increases in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and progesterone levels with decrease in calcium and phosphorous levels in treated group. In treated group, normal-size ovaries and more than one follicle on the ovaries were detected and pregnancy rate increased. In conclusion, CSFA supplementation was effective to maintain the reproductive performance when ewes were out of the breeding season. PMID:22629155

  13. Melatonin and resveratrol reverse the toxic effect of high boron (B) and modulate biochemical parameters in pepper plants (Capsicum annuum L.).

    Science.gov (United States)

    Sarafi, Eleana; Tsouvaltzis, Pavlos; Chatzissavvidis, Christos; Siomos, Anastasios; Therios, Ioannis

    2017-03-01

    The objectives of this research were to test a possible involvement of melatonin (MEL) and resveratrol (RES) in restoring growth and to control boron (B) toxicity in peppers. The plants were subjected to four different nutrient solution treatments as following: 1) half-strength Hoagland's nutrient solution (Control), 2) half-strength Hoagland's nutrient solution+100 μM B (100 μMB), 3) half-strength Hoagland's nutrient solution+100 μM boron+100 μMresveratrol (100 μMRES), and 4) half-strength Hoagland's nutrient solution+100 μM B+1 μMmelatonin (1 μM MEL). Pepper plants subjected to B excess (100 μM) for 68 days (d) exhibited visible B toxicity symptoms, reduced rate of photosynthesis (Pn) and reduced dry weight (DW), while their leaf and fruit had the greatest increase of B concentration. The reduction of photosynthesis was restored, the reduction of DW was prevented, while the B leaf and fruit accumulation was moderated with the application of both 100 μMresveratrol (RES) and 1 μMmelatonin (MEL). Moreover, plants exposed to MEL and/or RES displayed no visible B toxicity symptoms. The present study revealed a novel role of MEL and/or RES in the adaptation of pepper plants to B excess based on plant growth, physiological and biochemical criteria. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  14. Effect of Calcium Soap of Fatty Acids Supplementation on Serum Biochemical Parameters and Ovarian Activity during Out-of-the-Breeding Season in Crossbred Ewes

    Directory of Open Access Journals (Sweden)

    Hayat H. M. El-Nour

    2012-01-01

    Full Text Available This experiment aimed to evaluate the effect of calcium soap of fatty acid (CSFA supplementation on serum biochemical and hormones and ovarian activity during out-of-the-breeding season in ewes. Twelve crossbred ewes, 2-3 years of age and weighting 45–55 kg, were allocated into two equal groups. The first group was control and the other was treated with 50 g/head of CSFA. All ewes were fed basal diet and treated with 60 mg of medroxy progesterone acetate intravaginal sponge for 12 day. At the third day of sponge removal, the CSFA-treated group was given 50 g/head of CSFA daily for two estrous cycles. During the estrus phase, ovarian activity was detected using ultrasonography in both groups. All ewes were then subjected to natural breeding and conception rate. Blood samples were collected from all ewes during treatment period. Results revealed significant (<0.05 increases in serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, and progesterone levels with decrease in calcium and phosphorous levels in treated group. In treated group, normal-size ovaries and more than one follicle on the ovaries were detected and pregnancy rate increased. In conclusion, CSFA supplementation was effective to maintain the reproductive performance when ewes were out of the breeding season.

  15. Effect of exposure and withdrawal of 900-MHz-electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats.

    Science.gov (United States)

    Ragy, Merhan Mamdouh

    2015-01-01

    Increasing use of mobile phones in daily life with increasing adverse effects of electromagnetic radiation (EMR), emitted from mobile on some physiological processes, cause many concerns about their effects on human health. Therefore, this work was designed to study the effects of exposure to mobile phone emits 900-MHz EMR on the brain, liver and kidney of male albino rats. Thirty male adult rats were randomly divided into four groups (10 each) as follows: control group (rats without exposure to EMR), exposure group (exposed to 900-MHz EMR for 1 h/d for 60 d) and withdrawal group (exposed to 900-MHz electromagnetic wave for 1 h/d for 60 d then left for 30 d without exposure). EMR emitted from mobile phone led to a significant increase in malondialdehyde (MDA) levels and significant decrease total antioxidant capacity (TAC) levels in brain, liver and kidneys tissues. The sera activity of alanine transaminase (ALT), aspartate aminotransferase (AST), urea, creatinine and corticosterone were significantly increased (p electromagnetic field emitting from mobile phone might produce impairments in some biochemicals changes and oxidative stress in brain, liver and renal tissue of albino rats. These alterations were corrected by withdrawal.

  16. Effects of dietary dandelion extracts on growth performance, body composition, plasma biochemical parameters, immune responses and disease resistance of juvenile golden pompano Trachinotus ovatus.

    Science.gov (United States)

    Tan, Xiaohong; Sun, Zhenzhu; Chen, Shu; Chen, Silin; Huang, Zhong; Zhou, Chuanpeng; Zou, Cuiyun; Liu, Qingying; Ye, Huaqun; Lin, Heizhao; Ye, Chaoxia; Wang, Anli

    2017-07-01

    The present study was conducted to investigate the effects of dietary dandelion extracts (DE) supplementation on growth performance, feed utilization, body composition, plasma biochemical indices, immune responses, hepatic antioxidant abilities, and resistance to the pathogen Vibrio harveyi in Trachinotus ovatus. A basal diet supplemented with DE at 0, 0.50, 1.00, 2.00, 4.00 and 10.00 g kg -1 were fed to golden pompano for 8 weeks. The study indicated that dietary supplementation with DE could significantly improve final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed efficiency ratio (FER), feed intake (FI), protein efficiency ratio (PER) and protein deposit rate (PDR) (P Vibrio harveyi, significant higher post-challenge survival was observed in fish fed DE supplement (P growth performance, feed utilization, body protein deposit, immune ability, hepatic and plasma antioxidative enzyme activities and improve its resistance to infection by Vibrio harveyi. Copyright © 2017. Published by Elsevier Ltd.

  17. Comparing exposure zones by different exposure metrics using statistical parameters: contrast and precision.

    Science.gov (United States)

    Park, Ji Young; Ramachandran, Gurumurthy; Raynor, Peter C; Eberly, Lynn E; Olson, Greg

    2010-10-01

    Recently, the appropriateness of using the 'mass concentration' metric for ultrafine particles has been questioned and surface area (SA) or number concentration metrics has been proposed as alternatives. To assess the abilities of various exposure metrics to distinguish between different exposure zones in workplaces with nanoparticle aerosols, exposure concentrations were measured in preassigned 'high-' and 'low-'exposure zones in a restaurant, an aluminum die-casting factory, and a diesel engine laboratory using SA, number, and mass concentration metrics. Predetermined exposure classifications were compared by each metric using statistical parameters and concentration ratios that were calculated from the different exposure concentrations. In the restaurant, SA and fine particle number concentrations showed significant differences between the high- and low-exposure zones and they had higher contrast (the ratio of between-zone variance to the sum of the between-zone and within-zone variances) than mass concentrations. Mass concentrations did not show significant differences. In the die cast facility, concentrations of all metrics were significantly greater in the high zone than in the low zone. SA and fine particle number concentrations showed larger concentration ratios between the high and low zones and higher contrast than mass concentrations. None of the metrics were significantly different between the high- and low-exposure zones in the diesel engine laboratory. The SA and fine particle number concentrations appeared to be better at differentiating exposure zones and finding the particle generation sources in workplaces generating nanoparticles. Because the choice of an exposure metric has significant implications for epidemiologic studies and industrial hygiene practice, a multimetric sampling approach is recommended for nanoparticle exposure assessment.

  18. Influence of production system, age an sex on carcass parameters and some biochemical meat quality characteristics of reindeer (Rangifer tarandus tarandus L.

    Directory of Open Access Journals (Sweden)

    Sabine Sampels

    2005-03-01

    Full Text Available Carcass composition in reindeer is affected by feed intake and the age and sex of the animal. Studies have also shown that age, sex, carcass trim fat content and total intramuscular fat content (IMF influence lipid class composition. The aim of this study was to compare lipid class composition and IMF in relation to carcass weight, conformation and trim fat content, and to investigate how these parameters are affected by age, sex and different feed sources. Five groups of reindeer were studied. Two groups of calves were fed two grain-based pelleted feeds with different lipid compositions for approximately two months before slaughter. One of these groups was fed with conventional pellets, and the other with pellets enriched with linseed cake to increase the amount of n-3 fatty acids in the diet. Three groups of grazing reindeer were also included in the study, consisting of adult males, adult females or calves. Reindeer calves fed pellets had higher slaughter weights, higher trim fat content and better carcass conformation scores compared to the grazing calves. However, there was no significant difference in IMF between pellet-fed and grazing calves. Adult female reindeer had the highest and grazing calves the lowest slaughter weights, trim fat and IMF. There was no difference in lipid class composition in meat from calves fed with the two pelleted feeds, whereas grazing calves had a higher amount of phospholipids. Squalene was identified and quantified as a component of intramuscular lipids in reindeer meat. Effekt av produktionssystem, ålder och kön på slaktkroppskvalitet och några biokemiska egenskaper hos renköttAbstract in Swedish / Sammandrag: Slaktkroppssammansättningen hos renar påverkas av både foderintag, fodersammansättning och djurens ålder och kön. Tidigare har vi visat att renens kön, ålder, mängden intramuskulärt fett (IMF och putsfett på slaktkroppen påverkar sammansättningen av lipidklasser. Syftet med denna

  19. Comparison of biochemical and immunological profile of pediatric patients with acute myeloid leukemia in relation to healthy individuals

    Directory of Open Access Journals (Sweden)

    Fabiane L.F.Z. Sanches

    2015-09-01

    Conclusions: It was possible to characterize the biochemical and immunological profile of pediatric patients with AML, as well as highlight some significant differences in these parameters when comparing with healthy children and adolescents.

  20. [Effect of stress actions on some hematologic and biochemical parameters of rat blood and on energetic intermolecular interactions in lipid extract under effect of light radiation].

    Science.gov (United States)

    Zabelinskiĭ, S A; Chebotareva, M A; Tavrovskaia, T V; Skverchinskaia, E A; Shukoliukova, E P; Maslov, M N; Krivchenko, A I

    2012-01-01

    Comparative study has been carried of effect of the three-day long starvation, running, and their combination on morphological parameters of rat blood, lipid metabolism, and activity of blood Na,K-ATPase. Different effect has been shown of these stress factors on the blood erythrocyte composition. Starvation is accompanied by the most pronounced release of stored erythrocyte into blood, which results in a significant decrease both of the total amount of reticulocytes and the complete absence of reticulocytes of the I stage of maturity (the youngest). The running on treadmill led to a significant increase of the total amount of blood reticulocytes and to multiple increase of immature reticulocytes (RC-I and RC-II), which can indicate some stress of the bone marrow erythroid stem line. The curve of acid resistance of blood reticulocytes has shown the animal to experience the greatest stress at a combination of starvation and running. Starvation and running produced different effects on blood lipid characteristics. The content of triacylglycerides (TAG) in blood rose by 40% at starvation and decreased by 30% at running, a similar tendency being found for index of atherogeneity. The fatty acid composition of blood phospholipids at running and its combination with starvation practically did not differ from control. A change of Na,K-ATPase, which is so characteristic of reaction to various kinds of stress, sharply fell at starvation (by 22%), but increased at running (by 13%) and decreased markedly at combination of these actions. Absorption spectra of lipid extracts of the whole blood of the rats submitted to various stress actions showed that extracted from blood (at different amount depending on the kind of action) is an organic substance with coupled bonds, which absorbs light in the diapason of 360-620 nm. The absorption of light in the diapason of 400-410 nm has been found to belong to the Soret band of ferroheme and ferriheme. The shift of the Soret band indicates

  1. Identifying dominant controls on hydrologic parameter transfer from gauged to ungauged catchments: a comparative hydrology approach

    Science.gov (United States)

    Singh, R.; Archfield, S.A.; Wagener, T.

    2014-01-01

    Daily streamflow information is critical for solving various hydrologic problems, though observations of continuous streamflow for model calibration are available at only a small fraction of the world’s rivers. One approach to estimate daily streamflow at an ungauged location is to transfer rainfall–runoff model parameters calibrated at a gauged (donor) catchment to an ungauged (receiver) catchment of interest. Central to this approach is the selection of a hydrologically similar donor. No single metric or set of metrics of hydrologic similarity have been demonstrated to consistently select a suitable donor catchment. We design an experiment to diagnose the dominant controls on successful hydrologic model parameter transfer. We calibrate a lumped rainfall–runoff model to 83 stream gauges across the United States. All locations are USGS reference gauges with minimal human influence. Parameter sets from the calibrated models are then transferred to each of the other catchments and the performance of the transferred parameters is assessed. This transfer experiment is carried out both at the scale of the entire US and then for six geographic regions. We use classification and regression tree (CART) analysis to determine the relationship between catchment similarity and performance of transferred parameters. Similarity is defined using physical/climatic catchment characteristics, as well as streamflow response characteristics (signatures such as baseflow index and runoff ratio). Across the entire US, successful parameter transfer is governed by similarity in elevation and climate, and high similarity in streamflow signatures. Controls vary for different geographic regions though. Geology followed by drainage, topography and climate constitute the dominant similarity metrics in forested eastern mountains and plateaus, whereas agricultural land use relates most strongly with successful parameter transfer in the humid plains.

  2. Brachytherapy Improves Biochemical Failure–Free Survival in Low- and Intermediate-Risk Prostate Cancer Compared With Conventionally Fractionated External Beam Radiation Therapy: A Propensity Score Matched Analysis

    International Nuclear Information System (INIS)

    Smith, Graham D.; Pickles, Tom; Crook, Juanita; Martin, Andre-Guy; Vigneault, Eric; Cury, Fabio L.; Morris, Jim; Catton, Charles; Lukka, Himu; Warner, Andrew; Yang, Ying; Rodrigues, George

    2015-01-01

    Purpose: To compare, in a retrospective study, biochemical failure-free survival (bFFS) and overall survival (OS) in low-risk and intermediate-risk prostate cancer patients who received brachytherapy (BT) (either low-dose-rate brachytherapy [LDR-BT] or high-dose-rate brachytherapy with external beam radiation therapy [HDR-BT+EBRT]) versus external beam radiation therapy (EBRT) alone. Methods and Materials: Patient data were obtained from the ProCaRS database, which contains 7974 prostate cancer patients treated with primary radiation therapy at four Canadian cancer institutions from 1994 to 2010. Propensity score matching was used to obtain the following 3 matched cohorts with balanced baseline prognostic factors: (1) low-risk LDR-BT versus EBRT; (2) intermediate-risk LDR-BT versus EBRT; and (3) intermediate-risk HDR-BT+EBRT versus EBRT. Kaplan-Meier survival analysis was performed to compare differences in bFFS (primary endpoint) and OS in the 3 matched groups. Results: Propensity score matching created acceptable balance in the baseline prognostic factors in all matches. Final matches included 2 1:1 matches in the intermediate-risk cohorts, LDR-BT versus EBRT (total n=254) and HDR-BT+EBRT versus EBRT (total n=388), and one 4:1 match in the low-risk cohort (LDR-BT:EBRT, total n=400). Median follow-up ranged from 2.7 to 7.3 years for the 3 matched cohorts. Kaplan-Meier survival analysis showed that all BT treatment options were associated with statistically significant improvements in bFFS when compared with EBRT in all cohorts (intermediate-risk EBRT vs LDR-BT hazard ratio [HR] 4.58, P=.001; intermediate-risk EBRT vs HDR-BT+EBRT HR 2.08, P=.007; low-risk EBRT vs LDR-BT HR 2.90, P=.004). No significant difference in OS was found in all comparisons (intermediate-risk EBRT vs LDR-BT HR 1.27, P=.687; intermediate-risk EBRT vs HDR-BT+EBRT HR 1.55, P=.470; low-risk LDR-BT vs EBRT HR 1.41, P=.500). Conclusions: Propensity score matched analysis showed that BT options led

  3. Comparative Study of Plasma Parameters in Olive Ridley (Lepidochelys Olivacea and Hawksbill (Eretmochelys Imbricata During Nesting

    Directory of Open Access Journals (Sweden)

    A.Y.A. Alkindi

    2002-06-01

    Full Text Available The aim of this study is to investigate the role of plasma level parameters during nesting activity and provide data potentially useful to future studies on the dynamics of reproductive and stress hormones in the most endangered sea turtle species in the world. Plasma parameters in the sea turtles, olive ridley (Lipodochelys oliveacea and hawksbill (Eretmochelys imbricata from Masirah Island, Oman, were analyzed relative to nesting stress. To date, no study has been conducted on plasma parameter levels in sea turtles during nesting. Field observations were conducted under ideal temperature conditions. At the time of sampling, there was no significant difference for cloacal, sand, air or water temperature for the two species. Electrolytes (Cl¯, Ca++, K+, Na+ and Mg++, cholesterol, urea, uric acid and osmolarity were measured during nesting. Both species were observed to spend between 1.5 and 2.00 hours on the nesting grounds. Some had successful oviposition and completed all nesting phases, while others with incomplete nesting phases failed to oviposit their  eggs. Under both conditions, the turtles of both species had an exhaustive and stressful nesting exercise. Plasma parameter values, both intra-specifically and inter-specifically, were not significantly different for oviposited and non-oviposited turtles. This may indicate that both species have the same physiological adjustment relative to plasma parameters whether or not the turtles oviposited their eggs.

  4. Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus fingerlings

    Directory of Open Access Journals (Sweden)

    Shao Wei Zhai

    2014-07-01

    Full Text Available The present study was performed with tilapia (Oreochromis niloticus to evaluate the effects of diet supplementation with grape seed proanthocyanidins (GSPs on fish growth performance, some serum parameters and body composition. Three hundred tilapia fingerlings with the initial average body weight of 9.50±1.25 g were randomly divided into five treatment groups with four replicates in each group and 15 fish in each replicate. The dietary GSPs levels of five treatment groups were 0 (control group, 200, 400, 600, and 800 mg/kg, respectively. The trial period was 49 days. Growth performance parameters were significantly improved by GSPs supplementation (P<0.05, while survival rates were similar among all groups (P>0.05. Serum parameter results showed that activities of aminotransferase aspartate in 200 and 400 mg/kg GSPs groups and alanine aminotransferase in 400 mg/kg GSPs group were lowered significantly (P<0.05. Levels of triglyceride and total cholesterol (except 200 mg/kg GSPs group were significantly lowered, while lysozyme activity and albumin level were significantly higher in fish of GSPs supplemented groups, independently from the level of supplementation. The highest crude protein level and lowest crude lipid level were found in fish of all GSPs supplemented groups, while levels of moisture and ash in fish of all groups were similar (P>0.05. The results indicated that dietary 200 mg/kg GSPs could exert beneficial effects on growth and body composition of tilapia fingerlings, and ameliorate serum biochemistry parameters related to health status.

  5. [One-time effects of drinking mineral water and tap water enriched with silver nanoparticles on the biochemical markers of liver condition and metabolic parameters in healthy rats].

    Science.gov (United States)

    Efimenko, N V; Frolkov, V K; Kozlova, V V; Kaisinova, A S; Chalaya, E N

    2017-12-05

     The objective of the present research was to study the influence of tap water enriched with silver nanoparticles (NP) as well as that of «Krasnoarmeysky» and «Essentuki №17» mineral waters after their single administration through the oral gavage to the rats on the metabolism of carbohydrates and lipids, the biochemical markers of the liver condition, and the endocrine profile in the healthy animals.  The laboratory animals (130 male Wistar rats) were allocated to thirteen groups comprised of 10 rats each as follows: 1st group (n=10) intact animals, 2nd group (5 minutes after the administration of silver NP (n=10), 3rd group (15 minutes after the of silver NP), 4th group (60 minutes after the administration of silver NP), 5th group (n=10) (5 minutes after the introduction of the «Krasnoarmeysky» mineral water), 6th group (n=10) (15 min after the introduction of the «Krasnoarmeysky» mineral water), 7th group (n=10), (60 minutes after the introduction of the «Krasnoarmeysky» mineral water) 8th group (n=10) (5 minutes after the introduction of the «Essentuki № 17» mineral water), 9th group (n=10) (15 min after the introduction of the «Essentuki № 7» mineral water) , 10th group (n=10) (60 minutes after the introduction of the «Essentuki №17» mineral water), 11th group (n=10) (5 minutes after administration of tap water (control),12th group (n=10) (15 minutes after administration of tap water (control), and 13th (n=10) group 60 minutes after administration of tap water (control).  The study has demonstrated that the tap water enriched with silver nanoparticles similar to the mineral waters caused stress reactions that are inferior to those induced by «Essentuki №17» mineral water in terms of the magnitude; however, the effect provoked by the tap water was of longer duration. Moreover, the tap water enriched with silver nanoparticles stimulates prooxidant reactions, and inhibit the activity of antioxidant protection. Silver nanoparticles

  6. Comparing a simple methodology to evaluate hydrodynamic parameters with rainfall simulation experiments

    Science.gov (United States)

    Di Prima, Simone; Bagarello, Vincenzo; Bautista, Inmaculada; Burguet, Maria; Cerdà, Artemi; Iovino, Massimo; Prosdocimi, Massimo

    2016-04-01

    and, reasonably, it can better be represented by the high runs than the low runs (Alagna et al., 2015). Obviously, this methodology is also simpler than an approach involving soil characterization both before and after natural or simulated rainfall since it needs less equipment and field work. On the other hand, rainfall simulation experiments are more realistic and accurate, but also more sophisticated and costly (Cerdà, 1997). Rainfall simulation is often used to measure the infiltration process (e.g., Bhardwaj and Singh, 1992; Cerdà, 1999, 1997, 1996; Cerdà and Doerr, 2007; Iserloh et al., 2013; Liu et al., 2011; Tricker, 1979), and it has become an important method for assessing the subjects of soil erosion and soil hydrological processes (Iserloh et al., 2013). Its application allows a quick, specific and reproducible assessment of the meaning and impact of several factors, such as slope, soil type (infiltration, permeability), soil moisture, splash effect of raindrops (aggregate stability), surface structure, vegetation cover and vegetation structure (Bowyer-Bower and Burt, 1989). The objectives of this investigation are: (i) to compare infiltration rates measured by applying water at a relatively large distance from the soil surface with those obtained by rainfall simulation experiments and (ii) to verify if the Ks values determined with the BEST procedure are in line with the occurrence of runoff measured with a more robust methodology. Acknowledgements The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 603498 (RECARE project). References Alagna, V., Bagarello, V., Di Prima, S., Giordano, G., Iovino, M., 2015. Testing infiltration run effects on the estimated hydrodynamic parameters of a sandy-loam soil. Submitted to Geoderma. Bagarello, V., Castellini, M., Di Prima, S., Iovino, M., 2014. Soil hydraulic properties determined by infiltration experiments and

  7. Population parameters to compare dog breeds : differences between five Dutch purebred populations

    NARCIS (Netherlands)

    Nielen, A.L.J.; Beek, van der S.; Ubbink, G.J.; Knol, B.W.

    2001-01-01

    Differences in five purebred dog populations born in 1994 in the Netherlands were evaluated using different parameters. Numerically, the Golden Retriever was the largest breed (840 litters of 234 sires) and the Kooiker Dog (101 litters of 41 sires) the smallest. The litter per sire ratio was largest

  8. Haematological and Serum Biochemical Parameters of Broiler Chickens Fed Varying Dietary Levels of Fermented Castor Oil Seed Meal (Ricinus communis L. and Different Methionine Sources in South Western Nigeria

    Directory of Open Access Journals (Sweden)

    Ayorinde David Adeniran

    2017-09-01

    Full Text Available In this experiment, the effect of varying dietary levels of fermented castor oil seed meal (FCSM and different methionine sources (DL-methionine and herbal methionine on haematological and serum biochemical parameters of broilers. A total of 240 one-day-old Anak broiler chicks were used in the experiment lasted 56 days. The dietary experiment was laid out as a completely randomized design in a 4 × 2 factorial arrangement consisting of 4 dietary levels of FCSM (0, 50, 100 and 150 g/kg and 2 methionine sources (DL-methionine and herbal methionine. The birds were weighed and randomly distributed into 8 treatments with 3 replicates of 10 birds each. During the starter phase of the experiment, haemoglobin, red blood cell count, mean corpuscular haemoglobin concentration and eosinophil counts were higher (P

  9. Biochemical reactions of the organism

    International Nuclear Information System (INIS)

    Fedorova, A.V.

    1984-01-01

    Effects of mercury, strontium chloride, GMDA, trichlorfon as well as some radionuclides ( 89 Sr, 137 Cs, 203 Hg) were studied on rats. Changes in biochemical parameters (histamine content, activity of cholinesterase and histaminase) are noted. Most noticeable changes were observed in enzymatic activity. Distortion of enzymatic systems and accumulation of intermediate exchange and decay products of tissues in excess quantities affecting other systems can be the reason for changes in the organism. The observed changes in biochemical parameters should be necessarily taken into account at hygienic regulations of harmful effects of enviroment

  10. Dielectric and electro-optical parameters of two ferroelectric liquid crystals: a comparative study

    International Nuclear Information System (INIS)

    Kumar Misra, Abhishek; Kumar Srivastava, Abhishek; Shukla, J P; Manohar, Rajiv

    2008-01-01

    Dielectric relaxation and an electro-optical study of two ferroelectric liquid crystals having different spontaneous polarizations (Felix 16/100 and Felix 17/000) showing SmC* and SmA phases have been performed in the temperature range 30-80 compfn C. The experimental data have been used to determine different relaxation parameters, viz. distribution parameter, relaxation frequency, dielectric strength and rotational viscosity. The Goldstone mode of dielectric permittivity has been well observed for both the samples under investigation. The activation energy of both the samples has also been determined by the best theoretical fitting of the Arrhenius plot. We have also evaluated the optical response time and anchoring energy coefficients from electro-optical measurement techniques for these samples.

  11. Comparative parameters of solar cells for power generation: test stand implementation using DSP

    International Nuclear Information System (INIS)

    Álvarez López, Ramón Antonio; García Angarita, Maritza Andrea

    2014-01-01

    The technologies used in solar modules are distinguished mainly by th eenergy conversion efficiency. Consequently, the module selection is critic to the long term performance of photovoltaic generating facility. Therefore, the selection must be supported by experimental results obtained under a specific operation condition. The article implements an experimentally test for obtain the characteristic parameters of a solar module, we analyze the energy conversion efficiency and other correlated parameters that directly affect the performance of a photovoltaic generator. The results show that the use of a rapid prototyping system using open hardware, such as TMS320F28335 development kit makes it easy to build a test photovoltaic generation systems. Latter justified by the low cost of such devices and ease of programming. (author)

  12. Comparative analysis of morphogeometric parameters of forward cranial pole depending on type of a skull basis

    Directory of Open Access Journals (Sweden)

    Aleshkina О.Yu.

    2012-03-01

    Full Text Available The purpose of the work is comparison of parameters of a forward cranial pole depending on type of a skull basis. The research material contained 100 adult skulls divided into three craniotypes. The method of craniotopometry was used for measuring the parameters and further calculation of average value and their comparison among themselves. Results. The research helped to reveal that length of a forward cranial pole, length of a lateral part on the right and at the left, a corner f.c.-s-n prevail at flexibasilar craniotype. Conclusions. The width of a forward cranial pole, width of a lateral part on the right and at the left, a corner f.c.-n-g are more at platibasilar craniotype

  13. COMPARATIVE CHARACTERISTICS OF MORPHOMETRIC PARAMETERS OF DIFFERENT FORMS OF GIRLS FEET AGED 17-19

    Directory of Open Access Journals (Sweden)

    O.V. Konnova

    2009-03-01

    Full Text Available During examination of plantograms of the girls (n = 242 three forms of feet: egiptal, rectangular and greek have been distinguished, statistically characterized by reliable distinctions of separate linear and angular parameters and coefficient of the anterior part of the foot. Coefficients used for examination of the pathology of the foot fornix do not possess sufficient information and need additional examination of the dimensions of the angels of ankle-bone, calcaneal and navicular bones.

  14. Comparative study of trapping parameters of LiF(TLD-100) from different production batches

    Energy Technology Data Exchange (ETDEWEB)

    Bos, A.J.J.; Piters, T.M.; Vries, W. de; Hoogenboom, J.E. (Delft Univ. of Technology (Netherlands). Interfaculty Reactor Institute)

    1990-01-01

    Computerised glow curve analysis has been used to determine the trapping parameters of the main peaks of the thermoluminescent (TL) material LiF(TLD-100). The TL material (solid state chips) originated from six different production batches with at least 19 chips per batch. The maxima of glow peaks 2 to 5 are found at the same temperature within very small limits. The activation energy and frequency factor of the main glow peak (peak 5) of TLD-100 originating from two batches differ significantly from those of the other four investigated batches. Nevertheless, the sensitivity of glow peak 5 is more or less the same for all batches. The trapping parameters of glow peaks 2 to 4 of TLD-100 vary little from batch to batch. The measured half-life of peak 2 differed strongly from batch to batch. For all investigated peaks no correlation has been found between glow peak sensitivity and trapping parameters. The results of this study suggest that both defect concentration and nature of the trapping centres vary from batch to batch. It would appear that as a consequence of selection by the manufacturer, the differences between the batches in terms of total light output are small. (author).

  15. Protective Effects of Vitamin C (Ascorbic Acid in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

    Directory of Open Access Journals (Sweden)

    Alireza AYOUBI

    2015-01-01

    Full Text Available The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42 were randomly assigned into7 groups: I healthy; II fed lead acetate only; III vitamin C administered only; IV diabetic; V diabetic rats administered by vitamin C; VI diabetic rats given lead acetate and VII diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

  16. Biochemical characterization of the venom of the coral snake Micrurus tener and comparative biological activities in the mouse and a reptile model.

    Science.gov (United States)

    Bénard-Valle, Melisa; Carbajal-Saucedo, Alejandro; de Roodt, Adolfo; López-Vera, Estuardo; Alagón, Alejandro

    2014-01-01

    The objective of this study was to identify the venom components that could play a relevant role during envenomation caused by the coral snake Micrurus tener, through its biochemical characterization as well as the analysis of its effects on a murine model. Furthermore, it aimed to evaluate crude venom, in addition to its components, for possible specificity of action on a natural prey model (Conopsis lineata). The toxicity of the crude venom (delivered subcutaneously) showed a significant difference between the Median Lethal Dose (LD₅₀) in mice (4.4 μg/g) and in Conopsis lineata (12.1 μg/g) that was not observed when comparing the Median Paralyzing Dose (PD₅₀) values (mice = 4.7 μg/g; snakes = 4.1 μg/g). These results are evidence that the choice of study model strongly influences the apparent effects of crude venom. Moreover, based on the observed physical signs in the animal models, it was concluded that the most important physical effect caused by the venom is flaccid paralysis, which facilitates capture and subduing of prey regardless of whether it is alive; death is a logical consequence of the lack of oxygenation. Venom fractionation using a C18 reverse phase column yielded 35 fractions from which 16.6% caused paralysis and/or death to both animal models, 21.9% caused paralysis and/or death only to C. lineata and 1.6% were murine specific. Surprisingly, the diversity of snake-specific fractions did not reflect a difference between the PD₅₀s of the crude venom in mice and snakes, making it impossible to assume some type of specificity for either of the study models. Finally, the great diversity and abundance of fractions with no observable effect in snakes or mice (42.7%) suggested that the observed lethal fractions are not the only relevant toxic fractions within the venom and emphasized the possible relevance of interaction between components to generate the syndrome caused by the venom as a whole. Copyright © 2013 Elsevier Ltd. All rights

  17. Comparing the sustainability parameters of renewable, nuclear and fossil fuel electricity generation technologies

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Annette; Strezov, Vladimir; Evans, Tim

    2010-09-15

    The sustainability parameters of electricity generation have been assessed by the application of eight key indicators. Photovoltaics, wind, hydro, geothermal, biomass, natural gas, coal and nuclear power have been assessed according to their price, greenhouse gas emissions, efficiency, land use, water use, availability, limitations and social impacts on a per kilowatt hour basis. The relevance of this information to the Australian context is discussed. Also included are the results of a survey on Australian opinions regarding electricity generation, which found that Australian prefer solar electricity above any other method, however coal, biomass and nuclear power have low acceptance.

  18. Changes in visual quality, physiological and biochemical parameters assessed during the postharvest storage at chilling or non-chilling temperatures of three sweet basil (Ocimum basilicum L.) cultivars.

    Science.gov (United States)

    Fratianni, Florinda; Cefola, Maria; Pace, Bernardo; Cozzolino, Rosaria; De Giulio, Beatrice; Cozzolino, Autilia; d'Acierno, Antonio; Coppola, Raffaele; Logrieco, Antonio Francesco; Nazzaro, Filomena

    2017-08-15

    Leaves of three different sweet basil (Ocimum basilicum L.) cultivars (Italico a foglia larga, Cammeo, and Italiano classico) packed in macro-perforated polyethylene bags were stored at chilling (4°C) or non-chilling temperature (12°C) for 9days. During storage, visual quality, physiological (respiration rate, ethylene production, ammonium content) and chemical (antioxidant activity, total polyphenols and polyphenol profile) parameters were measured. Detached leaves stored at chilling temperature showed visual symptoms related to chilling injury, while ethylene production and ammonium content resulted associated to cultivar sensibility to damage at low temperature. Storage at 4°C caused a depletion in polyphenols content and antioxidant capability, which was preserved at 12°C. Regarding the polyphenols profile, stressful storage conditions did not enhance the phenolic metabolism. However, leaves stored at 12°C did not loss a significant amount of metabolites respect to fresh leaves, suggesting the possibility to extend the storability after the expiration date, for a possible recovery of bioactive compounds. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Evaluation of biochemical parameters and productive performance of japanese quail in response to the replacement of soybean meal with canola meal

    Directory of Open Access Journals (Sweden)

    Ali Asghar Saki

    2017-02-01

    Full Text Available The present study was conducted to evaluation the different levels canola meal (CM replacement by soybean meal (SBM in Japanese quails diets on productive performance, egg quality trails and blood parameters including blood protein, minerals concentration, liver enzymes and thyroid hormones. In this study, 160 of Japanese quails in second laying phase from 46 to 56 weeks of age were divided in four treatments with four replicates and 10 quail in each per replicate, on a completely randomized design (CRD.Treatments consisted:T1 control (without CM, T2, T3 and T4 replacing 30, 60 and 90% of SBM with CM respectively. The results were shown no significant variation in body weight between treatments in initial and end of the experiment (p > 0.05. There was significant decrease in feed intake by increasing level of CM (p 0.05. However, the egg shell thickness showed significantly decreased with increasing levels of canola meal in the diet  (p < 0.05. According to this study results, 30% replacement of SBM by CM in the diet (contained 10% CM has no adverse effect on quail’s performance.

  20. A comparative study on optimization of machining parameters by turning aerospace materials according to Taguchi method

    Directory of Open Access Journals (Sweden)

    Altin Abdullah

    2017-01-01

    Full Text Available The effects of cutting tool coating material and cutting speed on cutting forces and surface roughness were investigated by Taguchi experimental design. Main cutting force, Fz is considered as a criterion. The effects of machining parameters were investigated using Taguchi L18 orthogonal array. Optimal cutting conditions were determined using the signal-to-noise (S/N ratio which is calculated for average surface roughness and cutting force according to the “the smaller is better” approach. Using results of analysis of variance (ANOVA and signal-to-noise (S/N ratio, effects of parameters on both average surface roughness and cutting forces were statistically investigated. It was observed that feed rate and cutting speed had higher effect on cutting force in Hastelloy X, while the feed rate and cutting tool had higher effect on cutting force in Inconel 625. According to average surface roughness the cutting tool and feed rate had higher effect in Hastelloy X and Inconel 625.

  1. Description and comparative study of physico-chemical parameters of the teleost fish skin mucus.

    Science.gov (United States)

    Guardiola, Francisco A; Cuartero, María; Del Mar Collado-González, María; Arizcún, Marta; Díaz Baños, F Guillermo; Meseguer, José; Cuesta, Alberto; Esteban, María A

    2015-01-01

    The study of mucosal surfaces, and in particular the fish skin and its secreted mucus, has been of great interest recently among immunologists. Measurement of the viscosity and other physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolality and density) of the skin mucus can help to understand its biological functions. We have used five marine species of teleost: gilthead seabream (Sparus aurata L.), European sea bass (Dicentrarchus labrax L.), shi drum (Umbrina cirrosa L.), common dentex (Dentex dentex L.) and dusky grouper (Epinephelus marginatus L.), all of them with commercial interest in the aquaculture of the Mediterranean area. Mucus showed a direct shear- and temperature-dependent viscosity, with a non-Newtonian behavior, which differed however between two groups: one with higher viscosity (D. labrax, U. cirrosa, D. dentex) and the other with lower viscosity (S. aurata, E. marginatus). In addition, there was a clear interrelation between density and osmolality, as well as between density and temperature. Taking into account that high values of viscosity should improve the barrier effect against pathogens but low values of viscosity are needed for good locomotion characteristics, our results may help elucidate the relationship between physico-chemical and biological parameters of skin mucus, and disease susceptibility.

  2. The comparative effect of fasting with and without caloric restriction in Rat on oxidative stress parameters

    OpenAIRE

    Nurina Tyagita; Taufiqurrachman Nasihun; Titiek Sumarawati

    2016-01-01

    Introduction: Fasting, like Islamic Ramadan Fasting, has been associated with health benefits. Islamic Ramadan fasting, a form of caloric restriction (CR) or alternate day fasting that. Studies suggest a comparable effect of ADF and caloric restriction. Despite the fact that fasting can be considered as a form of dietary restriction, fasters tend to have difficulty to reduce their food intake during non-fasting period by overeating leading to the excessive calorie intake. To compare the effec...

  3. Effect of Weaning Age on Growth Performance, Feed Efficiency, Nutrient Digestibility and Blood-biochemical Parameters in Droughtmaster Crossbred Beef Calves.

    Science.gov (United States)

    Tao, Hui; Guo, Feng; Diao, Qi-Yu; Tu, Yan; Si, Bing-Wen; Xing, Yu-Chuan; Huang, De-Jun

    2017-11-03

    The objective of this study was to determine the effect of weaning age on intake, performance, nutrition metabolism and serum parameters of beef calves. 60 head of Droughtmaster crossbred calves were assigned to 5 groups with 12 calves in each group. The calves in control Group were remained with the dams till the 22-week age, while the calves weaned at 28d (4w), 42 d(6w), 56 d(8w) and 70 d(10w) of age were sent to Group 4W, Group6W, Group 8Wand Group 10W separately, and then they were fed on milk replacer (MR) till the 22-week age. DMI of calves in Group 4W was significantly lower than that in the rest groups from w17 to 22 (Pfeed efficiency of the calves were higher in the Group 4W and 6W than that in Group 6W and 8W from 11 to 13w (P0.05). Calves in Group 4W and 6W had lower body weight than that in the Group 8W and Group 10W and control group at 10-week age (Pcalves in Group 6W had no significant difference of body weight with that in control group, Group 8W and 10W(P0.05). Calve in Group 6W had higher final body weight and total gain than that in Group 4W, but no difference of total gain with that in Group 8W, 10W and control group. And weaning calve at age of 6-week age brought higher feed efficiency and average daily gain from w14 to 22, and higher DM and OM digestibility at w21. It is concluded that the weaning of calves at 6 weeks of age gave positive results.

  4. Effects of Ochratoxin A Feeding in White Leghorn Cockerels on Hematological and Serum Biochemical Parameters and its Amelioration with Silymarin and Vitamin E

    Directory of Open Access Journals (Sweden)

    Muhammad Fakhar-ud-Din Ahmad, Muhammad Kashif Saleemi*, Muhammad Zargham Khan, Faqir Muhammad1, Zahoor-ul-Hassan2, Aisha Khatoon, Sheraz Ahmed Bhatti, Rao Zahid Abbas3, Farzana Rizvi and Ishtiaq Ahmed4

    2012-10-01

    Full Text Available The objective of the present study was to evaluate the hematobiochemical effects tempted by ochratoxin A (OTA in White Leghorn (WL cockerels and to evaluate the effect of silymarin (SL, vitamin E (VE and their combination against OTA persuaded pathological alterations in cockerels. A total of 240 day-old WL cockerels were divided into 12 groups A-L having 20 birds in each group and group A was control. Two doses of OTA 1000 and 2000 µg/kg of feed were given to cockerels up to 7 weeks. Silymarin was administered at a dose rate of 10000 mg/kg and Vitamin E at a dose rate of 200 mg/kg alone and in combinations with two doses of OTA. In OTA treated groups total erythrocytes counts, leukocytes count, PCV and Hb were decreased as compared to control, SL and VE groups. Albumen and serum total proteins in OTA treated groups were significantly lower as compared to control, SL and VE groups. Serum alanine transferase was significantly increased in OTA fed groups in comparison with control, SL and VE groups. Creatinine and urea were increased in OTA treated groups but were almost normal in SL and VE groups. Results showed that OTA had severe effect on liver and kidney but SL and VE treated groups had normal liver and kidneys showing its hepatoprotective effects. However, at higher dose of OTA this ameliorative effect was partially observed. These agents may be recommended as a remedy for ochratoxicosis.

  5. Spontaneous and trigger-associated substorms compared: Electrodynamic parameters in the polar ionosphere

    Science.gov (United States)

    Liu, Jun-Ming; Zhang, Bei-Chen; Kamide, Y.; Wu, Zhen-Sen; Hu, Ze-Jun; Yang, Hui-Gen

    2011-01-01

    An attempt is made to study the difference, if any, between the response of the polar ionosphere to spontaneous substorms and that to trigger-associated substorms in terms of electrodynamic parameters including ionospheric current vectors, the electric potential, and the current function. The results show that, in the first approximation, the ionospheric parameters for the two types of substorms are quite similar. It is therefore conceived that spontaneous substorms are not very different from trigger-associated substorms in the development of substorm processes in the magnetosphere-ionosphere system. We demonstrate, however, that spontaneous substorms seem to have a more clearly identifiable growth phase, whereas trigger-associated substorms have a more powerful unloading process. Changes in the current intensity and the electric potential drop across the polar cap in the recovery phase are also quite different from each other. Both the current intensity and the cross-polar cap potential drop show a larger decrease in the recovery phase of trigger-associated substorms, but the potential drop decreases only slightly and the currents in the late morning sector are still strong for spontaneous substorms. We interpret these findings as an indication of the relative importance of the unloading process and the directly driven process in conjunction with the north-south polarity of the interplanetary magnetic field. There still exists a strong directly driven process in the recovery phase of spontaneous substorms. For trigger-associated substorms, however, both the directly driven process and the unloading process become weak after the peak time.

  6. A Comparative Survey on Parameters Influencing on Hexavalent Chromium Measurement as an Occupational Carcinogen

    Directory of Open Access Journals (Sweden)

    A. Tirgar

    2008-07-01

    Full Text Available Introduction & Objective: Hexavalent chromium, Cr+6, is a very harmful pollutant and a relatively unstable compound that is present in many industries. It is a known human respiratory carcinogen and occupational exposure to this chemical is associated with different health hazards. The purpose of this study was to evaluate the effects of four parameters including: type of sampling head, sampling height from the surface of electroplating solution, sampling duration, and sample storage duration on Cr+6 mist monitoring.Materials & Methods: To evaluate the influence of the main parameters as an experimental study, the 24 factorial design was applied at constant electroplating condition. A chromium electroplating bath with the ability to produce homogenous mist was used to create Cr+6 mist in laboratory setting. The National Institute for Occupational Safety and Health (NIOSH method 7600 was used to determine the Cr+6 concentration. Results: The results of 48 Cr+6 mist samples showed that Cr+6 concentration was higher: (1 for sampling by closed-face filter cassettes than for sampling by open-face filter cassettes (P<0.001; (2 for samples collected at 35 cm above the electroplating solution surface than for samples collected at 50 cm (P <0.001; (3 for sampling duration of 30 minutes than for sampling duration of 180 minutes (P <0.001; and, (4 for samples extracted immediately after sampling than for samples with delayed extraction (24 hours after sampling (P <0.001. Conclusion: It is concluded that the accuracy of Cr+6 mist sampling in electroplating shops will be enhanced when: (1 a closed-face filter cassette is used to prevent liquid splash contamination; (2 the sampling height is suitable as determined by further research; (3 the sampling duration is short (approximately 30 minutes; and, (4 the extraction of the Cr+6 sample is performed as soon as the sampling is completed.

  7. Can thyroid parotid ratio replace radioactive iodine uptake as a new objective parameter in the evaluation of thyroid function in cases of hyperthyroidism? A comparative study

    International Nuclear Information System (INIS)

    Malhotra, G.; Seshadri, N.; Gupta, A.; Das, B.K.; Gambhir, S.; Pradhan, P.K.; Bhagat, J.K.

    2002-01-01

    Aim: Radioactive iodine uptake (RAIU) measurements are used to distinguish other causes of thyrotoxicosis from hyperthyroidism. However it is a cumbersome technique requiring patients' visit for two or more days. Thyroid to parotid ratio (TP ratio) which is a ratio of Technetium-99m pertechnetate counts in thyroid to those in parotid glands is a new objective parameter for evaluation of thyroid function. The aim of present study was to compare TP ratio with RAIU in patients of thyrotoxicosis. Materials and Methods: The study group comprised of 15 patients (13 males and 2 females) who were clinically and biochemically hyperthyroid. All of these patients underwent Tc-99m pertechnetate thyroid scintigraphy followed by RAIU on two separate occasions. The technetium thyroid scan was done 20 minutes after intravenous application of 185 MBq (5 mCi) of the radiotracer. Both anterior and oblique views of the neck region were acquired under a standard large field of view gamma camera with LEAP collimation. The TP ratio was calculated from anterior view by drawing the region of interest over the thyroid and two regions of interest over each parotid gland. The counts of thyroid region were compared with each parotid and average of two sides was taken as TP ratio. The radioactive iodine uptake was estimated at 2,4,24, and 48 hours after oral administration of 925 KBq (25 microcuries) of I-131 in all patients with a thyroid probe by the standard technique. Apart from this 10 controls (5 males and 5 females) who were clinically and biochemically euthyroid were also included in the study and they underwent same studies as the cases. All patients with nodular disease of thyroid, past history of thyroiditis and parotitis were excluded from the study. Results: The scintigraphic findings in all patients revealed a diffusely concentrating thyroid gland with no evidence of hot or cold areas. The RAIU and TP ratio in both cases and controls is presented.: S.E: Standard error. There was a

  8. Compensation of kinematic geometric parameters error and comparative study of accuracy testing for robot

    Science.gov (United States)

    Du, Liang; Shi, Guangming; Guan, Weibin; Zhong, Yuansheng; Li, Jin

    2014-12-01

    Geometric error is the main error of the industrial robot, and it plays a more significantly important fact than other error facts for robot. The compensation model of kinematic error is proposed in this article. Many methods can be used to test the robot accuracy, therefore, how to compare which method is better one. In this article, a method is used to compare two methods for robot accuracy testing. It used Laser Tracker System (LTS) and Three Coordinate Measuring instrument (TCM) to test the robot accuracy according to standard. According to the compensation result, it gets the better method which can improve the robot accuracy apparently.

  9. The protective effect of royal jelly on chronic lambda-cyhalothrin toxicity: serum biochemical parameters, lipid peroxidation, and genotoxic and histopathological alterations in swiss albino mice.

    Science.gov (United States)

    Cavuşoğlu, Kültiğin; Yapar, Kürşad; Oruç, Ertan; Yalçın, Emine

    2011-10-01

    The present study was undertaken to investigate the protective effect of royal jelly (RJ) against toxicity induced by a synthetic pyrethroid insecticide, lambda-cyhalothrin (LCT), in Swiss albino mice. Animals were randomly divided into six groups of six animals each. The control group received distilled water alone, whereas mice in the treatment groups received RJ alone (100 or 250 mg/kg of body weight), LCT alone (668 ppm), or RJ+LCT for 21 days. All mice (100%) survived until the end of experiment and were sacrificed at the end of 24 hours. Blood, bone marrow, and liver and kidney tissues were analyzed for aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), and reduced glutathione (GSH) levels and micronucleus (MN) frequency, chromosomal aberrations (CAs), and pathological damages. Serum AST, ALT, BUN, and creatinine levels were elevated in mice treated with LCT alone compared with the other tested groups (P<.05). LCT-induced oxidative damage caused a significant decrease in GSH levels and a significant rise in MDA levels of liver and kidney tissues. LCT alone-treated mice presented higher frequencies (P<.05) of MNs, CAs, and abnormal metaphases compared with the controls; moreover, the mitotic index was lower than in controls (P<.05). Oral treatment with RJ significantly ameliorated the indices of hepatotoxicity, nephrotoxicity, lipid peroxidation, and genotoxicity induced by LCT. Both doses of RJ tested provided significant protection against LCT-induced toxicity, and its strongest effect was observed at the dose level of 250 mg/kg of body weight. In vivo results suggest that RJ is a potent antioxidant against LCT-induced toxicity, and its protective effect is dose dependent.

  10. Effects of buffering mineral mixtures on milk yield, milk composition, rumen pH and some blood biochemical parameters in heat stressed dairy cows

    Directory of Open Access Journals (Sweden)

    Adamović Milan

    2014-01-01

    group of cows, percentage of fat and dry substance in milk was significantly increased (p<0.05 and 0.01 respectively, while percentage of protein increase was insignificant. The substitution of magnesium oxide with bentonite had no impact on the values of examined parameters of metabolic profile, but it led to statistically significant increase of rumen contents pH values (p<0.05. From the obtained results it can be concluded that substitution of magnesium oxide with bentonite in feed mixtures that are used for feeding cows during summer period, can prevent rumen acidosis, which high yielding cows incline to under the conditions of elevated external temperatures. Besides that, this kind of substitution leads to improvement of milk composition, especially when percentage of fat and dry substance in milk is concerned. [Projekat Ministarstva nauke Republike Srbije, br. TR 31003 i br. TR 34013

  11. Effect of dietary supplementation of Ligustrum lucidum on performance, egg quality and blood biochemical parameters of Hy-Line Brown hens during the late laying period.

    Science.gov (United States)

    Li, X L; He, W L; Yang, M L; Yan, Y M; Xue, Y H; Zhao, S T

    2017-11-01

    The fruit of Ligustrum lucidum (FLL, Nuzhenzi in Chinese) is an important traditional medicine, and have attracted significant research attention because of their various biological activities. However, there are few research reports available on the use of FLL as a feed additive in livestock nutrition, particularly in layers. This study was conducted to determine the effects of supplementation of the diet of laying hens with FLL on laying performance, egg quality and blood metabolites. A total of 360 72-week-old hens were allocated to three dietary treatments (eight replications of 15 hens/treatment group) and were fed either a control diet or a diet supplemented with an inclusion level of 0.25% or 0.50% of FLL powder in the final feed, until 78 weeks of age. Hens were housed in a three-tier cage system. Feed and water were provided ad libitum. Blood samples and eggs were collected at the end of the experiment. The results showed that dietary supplementation with FLL did not affect egg weight, feed conversion ratio, eggshell thickness, albumen height, egg yolk color, eggshell breaking strength or egg shape index. However, FLL supplementation significantly decreased (Phens fed FLL compared with the control group. It can be concluded that FLL, at a supplementation level of 0.25% final feed, can be used as an effective feed additive to improve the performance of laying hens during the late laying period.

  12. Biochemical and toxicological studies of aqueous extract of ...

    African Journals Online (AJOL)

    Biochemical and toxicological studies of aqueous extract of Syzigium ... tract diseases and also used as food spices), on some biochemical indices, such as ... liver functions and blood parameters were studied in adult albino rats of both sexes.

  13. Comparative study of methods to estimate hydraulic parameters in the hydraulically undisturbed Opalinus Clay (Switzerland)

    Energy Technology Data Exchange (ETDEWEB)

    Yu, C.; Matray, J.-M. [Institut de Radioprotection et de Sûreté Nucléaire, Fontenay-aux-Roses, (France); Yu, C.; Gonçalvès, J. [Aix Marseille Université UMR 6635 CEREGE Technopôle Environnement Arbois-Méditerranée Aix-en-Provence, Cedex 4 (France); and others

    2017-04-15

    The deep borehole (DB) experiment gave the opportunity to acquire hydraulic parameters in a hydraulically undisturbed zone of the Opalinus Clay at the Mont Terri rock laboratory (Switzerland). Three methods were used to estimate hydraulic conductivity and specific storage values of the Opalinus Clay formation and its bounding formations through the 248 m deep borehole BDB-1: application of a Poiseuille-type law involving petrophysical measurements, spectral analysis of pressure time series and in situ hydraulic tests. The hydraulic conductivity range in the Opalinus Clay given by the first method is 2 × 10{sup -14}-6 × 10{sup -13} m s{sup -1} for a cementation factor ranging between 2 and 3. These results show low vertical variability whereas in situ hydraulic tests suggest higher values up to 7 × 10{sup -12} m s{sup -1}. Core analysis provides economical estimates of the homogeneous matrix hydraulic properties but do not account for heterogeneities at larger scale such as potential tectonic conductive features. Specific storage values obtained by spectral analysis are consistent and in the order of 10{sup -6} m{sup -1}, while formulations using phase shift and gain between pore pressure signals were found to be inappropriate to evaluate hydraulic conductivity in the Opalinus Clay. The values obtained are globally in good agreement with the ones obtained previously at the rock laboratory. (authors)

  14. A comparative study of salt tolerance parameters in 11 wild relatives of Arabidopsis thaliana

    KAUST Repository

    Orsini, Francesco

    2010-07-01

    Salinity is an abiotic stress that limits both yield and the expansion of agricultural crops to new areas. In the last 20 years our basic understanding of the mechanisms underlying plant tolerance and adaptation to saline environments has greatly improved owing to active development of advanced tools in molecular, genomics, and bioinformatics analyses. However, the full potential of investigative power has not been fully exploited, because the use of halophytes as model systems in plant salt tolerance research is largely neglected. The recent introduction of halophytic Arabidopsis-Relative Model Species (ARMS) has begun to compare and relate several unique genetic resources to the well-developed Arabidopsis model. In a search for candidates to begin to understand, through genetic analyses, the biological bases of salt tolerance, 11 wild relatives of Arabidopsis thaliana were compared: Barbarea verna, Capsella bursa-pastoris, Hirschfeldia incana, Lepidium densiflorum, Malcolmia triloba, Lepidium virginicum, Descurainia pinnata, Sisymbrium officinale, Thellungiella parvula, Thellungiella salsuginea (previously T. halophila), and Thlaspi arvense. Among these species, highly salt-tolerant (L. densiflorum and L. virginicum) and moderately salt-tolerant (M. triloba and H. incana) species were identified. Only T. parvula revealed a true halophytic habitus, comparable to the better studied Thellungiella salsuginea. Major differences in growth, water transport properties, and ion accumulation are observed and discussed to describe the distinctive traits and physiological responses that can now be studied genetically in salt stress research. 2010 The Author.

  15. Effect of graded levels of dietary seaweed (Ulva sp. on growth, hematological and serum biochemical parameters in dusky kob, Argyrosomus japonicus, sciaenidae

    Directory of Open Access Journals (Sweden)

    Molatelo Junior Madibana

    2017-09-01

    Full Text Available This study was designed to assess the effect of incorporating graded levels of green macroalgae seaweed (Ulva sp. into diets of juvenile dusky kob (Argyrosomus japonicus on growth performance, hematology and serum biochemistry. Five experimental diets were formulated to contain 0 (Ulva0, 50 (Ulva50, 100 (Ulva100, 150 (Ulva150 and 200 (Ulva200 g seaweed meal/kg commercial kob feed on a dry matter basis. Seventy-seven dusky kob fingerlings (mean body mass 9.14 ± 0.30 g were distributed into each of 20 experimental tanks and offered diets at 2.8% body mass. Weight and length of fish were measured weekly for 9 weeks. At the end of the feeding trial, fish fed Ulva50 had higher body mass (38.20 ± 1.38 g compared to those fed the other three Ulva-based diets. Fish offered Ulva200 had significantly (P  .05 by dietary treatments. However, the levels of both blood cholesterol and alkaline phosphatase tended to decrease as dietary Ulva inclusion levels increased. These results indicate that Ulva could be incorporated at the rate of 50 g kg−1 in future commercial dusky kob diets without any negative effects on feed utilizationa and growth performance. Hematology and serum biochemistry results indicate that dietary inclusion of Ulva had no adverse effects on physiological status of the fish. Keywords: Ulva sp., Haematology, Dusky kob, Feed conversion efficiency, Specific growth rate

  16. DYNAMICS OF CLINICAL AND BIOCHEMICAL PARAMETERS AND FUNCTIONAL STATE OF THE AUTONOMIC NERVOUS SYSTEM IN PATIENTS WITH ACUTE HEPATITIS B WITH CHRONIC ALCOHOL USE IN HEPATOTOXIC DOSES

    Directory of Open Access Journals (Sweden)

    O. O. Furyk

    2014-02-01

    convalescence maintaining higher (p<0,05, alanine aminotransferase activity compared with group II patients. 2. Acute period of acute hepatitis B regardless of whether chronic alcohol use in hepatotoxic doses is characterized by the development of autonomic imbalance towards vagotonia. 3. Period of convalescence in patients with acute hepatitis B with chronic alcohol use in hepatotoxic doses is accompanied by more severe autonomic dysfunction in 33,6% (p<0,05.

  17. Dentin hypersensitivity clinical study comparing LILT and LEDT keeping the same irradiation parameters

    International Nuclear Information System (INIS)

    Lizarelli, R F Z; Miguel, F A C; Bagnato, V S; Freitas-Pontes, K M; Villa, G E P; Nunez, S C

    2010-01-01

    Dentin hypersensitivity is a common condition associated with high dental pain. A new LED-based (light emitting diode) light source has been used as an experimental tool in some studies. Purpose: The main objective was to compare these two light sources emitting in the same spectral band (red – from 625 to 660 nm) to promote pain relief. Material and methods: A total of 6 sessions were accomplished, being three irradiation sessions and three follow-up sessions. This single-blind study compared a control group (Placebo) and two other groups with different equipments: low laser intensity treatment (LILT) and a light emitting diode system treatment (LEDT). Results: The results showed that there is no statistical difference between LILT and LEDT groups, however, both were better than control group (p ≤ 0.01) in terms of treatment efficiency; there is no difference between the second and the third sessions for both treatment, it means that the third session was not necessary; finally, the improvement at the end of the entire research (follow up care of 30 days) was very expressive in comparison to pre-treatment situation for all teeth (p ≤ 0.01). Conclusion: LILT and LEDT were equally effective to treat dentine hypersensitivity, a 3rd treatment session was not necessary/two sessions are enough

  18. Longitudinal left ventricular function for prediction of survival in systemic light-chain amyloidosis: incremental value compared with clinical and biochemical markers.

    Science.gov (United States)

    Buss, Sebastian J; Emami, Mostafa; Mereles, Derliz; Korosoglou, Grigorios; Kristen, Arnt V; Voss, Andreas; Schellberg, Dieter; Zugck, Christian; Galuschky, Christian; Giannitsis, Evangelos; Hegenbart, Ute; Ho, Anthony D; Katus, Hugo A; Schonland, Stefan O; Hardt, Stefan E

    2012-09-18

    The aim of the study was to determine whether longitudinal left ventricular (LV) function provides prognostic information in a large cohort of patients with systemic light-chain (AL) amyloidosis. AL amyloidosis is associated with a high incidence of cardiovascular events. Reduced myocardial longitudinal function is one of the hallmarks of myocardial involvement in this rare disease. Two hundred six consecutive patients with biopsy-proven AL amyloidosis were investigated in this prospective observational study. Echocardiographic imaging parameters, mean tissue Doppler-derived longitudinal strain (LS), and two-dimensional global longitudinal strain (2D-GLS) of the LV, cardiac serological biomarkers, and comprehensive clinical disease characteristics were assessed. The primary endpoint was all-cause mortality or heart transplantation. After a median follow-up of 1207 days, LS and 2D-GLS were significant predictors of survival in AL amyloidosis. The cutoff values discriminating survivors from nonsurvivors were -10.65% for LS and -11.78% for 2D-GLS. In a multivariable echocardiographic Cox model, only diastolic dysfunction and 2D-GLS remained as independent predictors of survival. In comprehensive clinical models, 2D-GLS (p < 0.0001), diastolic dysfunction (p < 0.01), the pathologic free light chains (p < 0.05), cardiac troponin-T (cTnT) (p < 0.01), and the Karnofsky index (p < 0.001) remained as independent predictors. 2D-GLS delineated a superior prognostic value compared with that derived from pathologic free light chains or cTnT in patients evaluated before firstline chemotherapy (n = 113; p < 0.0001), and remained the only independent predictor besides the Karnofsky index in subjects with preserved LV ejection fraction (≥50%; n = 127; p < 0.01). LS and 2D-GLS both offered significant incremental information (p < 0.001) for the assessment of outcome compared with clinical variables (age, Karnofsky index, and New York Heart Association functional class) and

  19. Comparative analysis of competitive activity parameters of amateur boxers high qualification

    Directory of Open Access Journals (Sweden)

    V.P. Martsiv

    2014-12-01

    Full Text Available Purpose : analyze competitive activity of boxers of high qualifications in different time periods in the development of boxing. Material : 142 analyzed boxing matches at the Olympic Games 2012. Results : present indicators of competitive activity of boxers. Shows the comparative characteristics of the battles that took place under different formulas of competitive activity in different periods of the development of amateur boxing. It is shown that increasing the skills of athletes appear to increase the density of hits in combat. As a result of innovations in the rules of the competition, boxing match was different higher intensity combat, attacking actions began to perform at a higher speed mode. Conclusions : As a result of the transition to the new formula of fighting to increase the density of the match. Also a 3-fold increase in the number of strikes indicator, surviving on target. Increased efficiency factor strikes, which leads to the expansion of effective technical and tactical actions.

  20. The comparative effect of fasting with and without caloric restriction in Rat on oxidative stress parameters

    Directory of Open Access Journals (Sweden)

    Nurina Tyagita

    2016-09-01

    Full Text Available Introduction: Fasting, like Islamic Ramadan Fasting, has been associated with health benefits. Islamic Ramadan fasting, a form of caloric restriction (CR or alternate day fasting that. Studies suggest a comparable effect of ADF and caloric restriction. Despite the fact that fasting can be considered as a form of dietary restriction, fasters tend to have difficulty to reduce their food intake during non-fasting period by overeating leading to the excessive calorie intake. To compare the effect of fasting with and without caloric restriction in Sprague Dawley rats. Methods: The rats were assigned to one of three groups: ADF with 70 % calorie intake (30% CR, ADF with 100 % calorie intake (0% CR, and ADF with 140 % calorie intake (excessive calorie intake and AL (fed ad libitum. All groups were subjected to 6 hour fasting per day (9 a.m. until 3 p.m. or 15 days. The plasma sample was taken for MDA level assessment. Urinary 8-oxodG levels were determined by using ELISA. Results: ADF with 30% calorie restriction (F70 group had the lowest MDA level. Measurement of 8-oxodG level showed that group F70 had the highest production of 8-oxodG. There was an inverse relationship between MDA level and 8-oxodG level meaning the lower MDA level, the lower 8-oxodG levels were produced. Conclusion: ADF fasting with 30% caloric restriction reduce the MDA level but increase 8-oxodG levels. This study suggest the beneficial effect of fasting requires decrease in overall caloric intake.

  1. Oncoplastic round block technique has comparable operative parameters as standard wide local excision: a matched case-control study.

    Science.gov (United States)

    Lim, Geok-Hoon; Allen, John Carson; Ng, Ruey Pyng

    2017-08-01

    Although oncoplastic breast surgery is used to resect larger tumors with lower re-excision rates compared to standard wide local excision (sWLE), criticisms of oncoplastic surgery include a longer-albeit, well concealed-scar, longer operating time and hospital stay, and increased risk of complications. Round block technique has been reported to be very suitable for patients with relatively smaller breasts and minimal ptosis. We aim to determine if round block technique will result in operative parameters comparable with sWLE. Breast cancer patients who underwent a round block procedure from 1st May 2014 to 31st January 2016 were included in the study. These patients were then matched for the type of axillary procedure, on a one to one basis, with breast cancer patients who had undergone sWLE from 1st August 2011 to 31st January 2016. The operative parameters between the 2 groups were compared. 22 patients were included in the study. Patient demographics and histologic parameters were similar in the 2 groups. No complications were reported in either group. The mean operating time was 122 and 114 minutes in the round block and sWLE groups, respectively (P=0.64). Length of stay was similar in the 2 groups (P=0.11). Round block patients had better cosmesis and lower re-excision rates. A higher rate of recurrence was observed in the sWLE group. The round block technique has comparable operative parameters to sWLE with no evidence of increased complications. Lower re-excision rate and better cosmesis were observed in the round block patients suggesting that the round block technique is not only comparable in general, but may have advantages to sWLE in selected cases.

  2. The logic of comparative life history studies for estimating key parameters, with a focus on natural mortality rate

    Science.gov (United States)

    Hoenig, John M; Then, Amy Y.-H.; Babcock, Elizabeth A.; Hall, Norman G.; Hewitt, David A.; Hesp, Sybrand A.

    2016-01-01

    There are a number of key parameters in population dynamics that are difficult to estimate, such as natural mortality rate, intrinsic rate of population growth, and stock-recruitment relationships. Often, these parameters of a stock are, or can be, estimated indirectly on the basis of comparative life history studies. That is, the relationship between a difficult to estimate parameter and life history correlates is examined over a wide variety of species in order to develop predictive equations. The form of these equations may be derived from life history theory or simply be suggested by exploratory data analysis. Similarly, population characteristics such as potential yield can be estimated by making use of a relationship between the population parameter and bio-chemico–physical characteristics of the ecosystem. Surprisingly, little work has been done to evaluate how well these indirect estimators work and, in fact, there is little guidance on how to conduct comparative life history studies and how to evaluate them. We consider five issues arising in such studies: (i) the parameters of interest may be ill-defined idealizations of the real world, (ii) true values of the parameters are not known for any species, (iii) selecting data based on the quality of the estimates can introduce a host of problems, (iv) the estimates that are available for comparison constitute a non-random sample of species from an ill-defined population of species of interest, and (v) the hierarchical nature of the data (e.g. stocks within species within genera within families, etc., with multiple observations at each level) warrants consideration. We discuss how these issues can be handled and how they shape the kinds of questions that can be asked of a database of life history studies.

  3. Parâmetros clínicos, hematológicos, bioquímicos e hormonais de bovinos submetidos ao estresse calórico Clinical, hematological, biochemical, and hormonal parameters of cattle submitted to heat stress

    Directory of Open Access Journals (Sweden)

    F. Ferreira

    2009-08-01

    Full Text Available Caracterizaram-se as respostas clínicas, hematológicas, bioquímicas e hormonais de 71 bovinos F2 (½ Gir x ½ Holandês, 39 machos e 32 fêmeas, entre 14 e 20 meses de idade, submetidos a condições de termoneutralidade (22ºC a 70% de umidade relativa e ao estresse calórico (42ºC e 60% de umidade relativa. Os tratamentos foram realizados nas estações de verão e inverno em câmara bioclimática. Ao exame clínico, todos os animais apresentaram salivação e retração de globo ocular. Os animais apresentaram aumento do número de eritrócitos, concentração de hemoglobina, hematócrito, proteínas totais, ureia, creatinina, sódio, potássio, cloretos, cortisol, pH e densidade urinária e matéria seca das fezes no período de estresse calórico (P0,05. Concluiu-se que as variáveis hematológicas, bioquímicas e hormonais, bem como as características fenotípicas, revelaram-se importantes para caracterizar o estresse calórico em bovinos.Clinical, haemathological, biochemical, and hormonal parameters were characterized in 39 males and 32 females crossbred cattle (½ Holstein x ½ Gir, averaging 14 to 20 months of age, submitted to thermoneutrality (22ºC and 70% of RH and heat stress (42ºC and 60% RH in summer and winter in climatic chamber. At clinical examination, all animals presented salivation and ocular globe retraction. Under heat stress, animals increased erythrocytes count, hemoglobin total concentration, hematocrit, concentraction of total proteins, urea, creatinin, sodium, potassium, chlorides, cortisol, urinary pH and density, and dry matter of feces (P0.05. It was concluded that hematology, biochemical, and hormonal parameters are important to characterize the heat stress in cattle.

  4. Beyond leaf color: Comparing camera-based phenological metrics with leaf biochemical, biophysical, and spectral properties throughout the growing season of a temperate deciduous forest

    Science.gov (United States)

    Yang, Xi; Tang, Jianwu; Mustard, John F.

    2014-03-01

    Plant phenology, a sensitive indicator of climate change, influences vegetation-atmosphere interactions by changing the carbon and water cycles from local to global scales. Camera-based phenological observations of the color changes of the vegetation canopy throughout the growing season have become popular in recent years. However, the linkages between camera phenological metrics and leaf biochemical, biophysical, and spectral properties are elusive. We measured key leaf properties including chlorophyll concentration and leaf reflectance on a weekly basis from June to November 2011 in a white oak forest on the island of Martha's Vineyard, Massachusetts, USA. Concurrently, we used a digital camera to automatically acquire daily pictures of the tree canopies. We found that there was a mismatch between the camera-based phenological metric for the canopy greenness (green chromatic coordinate, gcc) and the total chlorophyll and carotenoids concentration and leaf mass per area during late spring/early summer. The seasonal peak of gcc is approximately 20 days earlier than the peak of the total chlorophyll concentration. During the fall, both canopy and leaf redness were significantly correlated with the vegetation index for anthocyanin concentration, opening a new window to quantify vegetation senescence remotely. Satellite- and camera-based vegetation indices agreed well, suggesting that camera-based observations can be used as the ground validation for satellites. Using the high-temporal resolution dataset of leaf biochemical, biophysical, and spectral properties, our results show the strengths and potential uncertainties to use canopy color as the proxy of ecosystem functioning.

  5. Biofertilizing efficiency of Sargassum polycystum extract on growth and biochemical composition of Vigna radiata and Vigna mungo

    Directory of Open Access Journals (Sweden)

    B Bharath

    2018-01-01

    Full Text Available Objective: To evaluate the effect of marine brown alga Sargassum polycystum extract on growth and biochemical parameters of Vigna radiata and Vigna mungo.Methods: Different concentrations of algal extracts (0.5%, 1.0%, 2.0%, 3.0%, 4.0%, and 5.0% were prepared and applied to the crops at every 10-day intervals under natural conditions. After 30 d, the plants were harvested to evaluate the growth and biochemical parameters.Results: Seaweed liquid fertilizers treated seedlings showed maximum growth in 3.0% concentration when compared to the untreated seedlings. Similarly, biochemical parameters such as photosynthetic pigments, protein, reducing sugar, total sugar and amino acids exhibited increases in 3.0% concentration seaweed extract. Decreases in growth and biochemical parameters were noticed in concentrations higher than 3.0%.Conclusions: Presence of micronutrients and growth regulating substances in the liquid extract help healthier and faster productivity of the crop.

  6. Efeito da bebida de café descascado sobre a atividade antioxidante, os parâmetros hematológicos e bioquímicos em ratos Peeled coffee brew effect in the antioxidant activity hematological and biochemical parameters in rats

    Directory of Open Access Journals (Sweden)

    Stella Maris da Silveira Duarte

    2009-12-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da bebida filtrada, preparada com café-arábica descascado, sobre a peroxidação de lipídios, os parâmetros hematológicos e bioquímicos in vivo. Para isso foram utilizados ratos que ingeriram 280 mg/kg/dia da bebida de café por 7 dias (tratamento agudo e 30 dias (tratamento crônico. A fim de determinar se a bebida de café é capaz de reduzir o estresse oxidativo, foi analisada a peroxidação de lipídios isolados de cérebro de rato analisando-se as substâncias reativas do ácido tiobarbitúrico. A ingestão da bebida por 7 e 30 dias inibiu significativamente a peroxidação lipídica (p The aim of this study was to verify the effect of filtered coffee brew prepared with peeled Coffea arabica on the lipid peroxidation and on hematological and biochemical parameters in vivo. Rats were ingested 280 mg/kg/day of coffee brew for 7 days (acute treatment and 30 days (chronic treatment. In order to determine whether coffee can reduce the oxidative stress, the rats brain isolated lipid peroxidation was accessed evaluating the thiobarbituric acid-reactive substances (TBARs.The ingestion of coffee brew for 7 and 30 days inhibited, significantly, the malondialdehyde concentration (p 0.05 between the percentage of peroxidation inhibition when the acute (48.6% and chronic (53.4% treatments were compared. The chronic ingestion of moderate doses of filtered coffee brew did not modify the plasma level of the hematological and biochemical parameters analyzed. The results indicate the beneficial health effect of moderated filtered coffee brew ingestion since it inhibited lipid peroxidation, but did not change the rats cholesterol and triacylglycerol plasma levels.

  7. A comparative study of EVA with and without thermal history for different lamination process parameters

    Energy Technology Data Exchange (ETDEWEB)

    Drabczyk, Kazimierz, E-mail: kazimierz.drabczyk@wp.pl [Institute of Metallurgy and Materials Science, 25 Reymonta St., 30-059 Krakow (Poland); Panek, Piotr [Institute of Metallurgy and Materials Science, 25 Reymonta St., 30-059 Krakow (Poland)

    2012-09-01

    In more than 80% of the worldwide photovoltaic (PV) modules, mostly very fragile and 200 {mu}m thick, crystalline silicon solar cells are encapsulated into ethylene-vinyl acetate (EVA) foils, which bond the module components together, provide physical protection, electrical insulation and a barrier for moisture ingress. The understanding of what can happen with EVA during its transport, storage and lamination process is necessary to optimize the quality of the PV module for its long exposure to outdoor weather conditions. Achieving a proper cross-link density of over 70%, it is essential to overcome the cold flow of EVA and to make the module durable. In this work, the feasibility of the use of differential scanning calorimetry (DSC) compared with the solvent extraction (SE) method by toluene were evaluated in order to provide structural information on the EVA curing kinetics and the cross-link density. DSC tests were performed on a DTA DuPont1600 tester. The temperature range for the test was from -50 Degree-Sign C to 200 Degree-Sign C, with the heating rate of 10 Degree-Sign C/min, and the endothermic and exothermic peaks were evaluated. Toluene solvent extractions were performed on the same set of samples that were analyzed by DSC. The measured cross-link density shows a direct dependence on the pre-lamination conditions of EVA, which is in good agreement with the data obtained with the DSC method.

  8. A comparative study of EVA with and without thermal history for different lamination process parameters

    International Nuclear Information System (INIS)

    Drabczyk, Kazimierz; Panek, Piotr

    2012-01-01

    In more than 80% of the worldwide photovoltaic (PV) modules, mostly very fragile and 200 μm thick, crystalline silicon solar cells are encapsulated into ethylene-vinyl acetate (EVA) foils, which bond the module components together, provide physical protection, electrical insulation and a barrier for moisture ingress. The understanding of what can happen with EVA during its transport, storage and lamination process is necessary to optimize the quality of the PV module for its long exposure to outdoor weather conditions. Achieving a proper cross-link density of over 70%, it is essential to overcome the cold flow of EVA and to make the module durable. In this work, the feasibility of the use of differential scanning calorimetry (DSC) compared with the solvent extraction (SE) method by toluene were evaluated in order to provide structural information on the EVA curing kinetics and the cross-link density. DSC tests were performed on a DTA DuPont1600 tester. The temperature range for the test was from −50 °C to 200 °C, with the heating rate of 10 °C/min, and the endothermic and exothermic peaks were evaluated. Toluene solvent extractions were performed on the same set of samples that were analyzed by DSC. The measured cross-link density shows a direct dependence on the pre-lamination conditions of EVA, which is in good agreement with the data obtained with the DSC method.

  9. Hematological and Serum Biochemical Parameters of Weanling ...

    African Journals Online (AJOL)

    The objective of this study was to determine the dose-dependent effect of medroxyprogesterone acetate on hematology and serum biochemistry in weanling dogs. Nine healthy local dogs between 12 to 14 weeks of age were randomly assigned to three treatments: control, 150mg/kg and 300mg/kg of medroxyprogesterone ...

  10. Incidence and biochemical parameters of dermatophilosis in ...

    African Journals Online (AJOL)

    Ibrahim Eldaghayes

    2018-01-22

    Jan 22, 2018 ... major cattle diseases of immense economic importance ravaging Nigerian ... lesions of dermatophilosis and these arthropod vectors are important in the ..... serum alkaline phosphatase activity in beagle dogs. Exp. Anim. 6(4) ...

  11. Evaluation of Haematological and Biochemical Parameters of ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Keywords: Crude oil, Afiesere oil field, blood, toxicity, water quality, Oreochromis niloticus. Crude oil exploration and ..... impaired kidney function, liver diseases and cardiac ... moench on acetaminophen-induced hepatoxicity in mice. AM.J. Uin ...

  12. Short Communication Biochemical Nutritional Parameters in Breast ...

    African Journals Online (AJOL)

    2011-04-07

    Apr 7, 2011 ... The production of human milk (lactogenesis) is dependent on factors in ... assessed the impact which HIV infection might have on the nutritional quality of human breast milk and plasma ..... Developing Gut and of Human Milk.

  13. Growth, ectomycorrhization and biochemical parameters of Quercus ...

    African Journals Online (AJOL)

    Drought conditions are the major constraint to the early establishment of Quercus suber species. However, drought responses of this species depend on provenances. The objective of this study was to obtain more comprehensive knowledge on the influence of drought conditions on the response of Q. suber L. seedlings ...

  14. Growth, ectomycorrhization and biochemical parameters of Quercus ...

    African Journals Online (AJOL)

    daoudi

    2016-09-21

    Sep 21, 2016 ... Summer drought in the Mediterranean region is characterized by 4 months dry period with little or no precipitations, high temperatures and high irradiance, considering the major constraints for vegetation. (Aussenac, 2000; Faria et al., 1999). Mediterranean species are thus, often exposed to water stress ...

  15. Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants.

    Science.gov (United States)

    Kamdem, Irénée; Hiligsmann, Serge; Vanderghem, Caroline; Bilik, Igor; Paquot, Michel; Thonart, Philippe

    2013-12-01

    We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the "Williams Cavendish" banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles-midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 ml g⁻¹ dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 ml g⁻¹, respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media. The bioconversion yields for each MP were below 50 %, showing that these substrates were not fully biodegraded after 188 days. The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation-Del Monte plantations for 188 days is approximately 10.5 × 10⁶ kW h (which would be worth 0.80-1.58 million euros in the current market). This bioenergy could serve the requirements of about 42,000 people in the region, although CH₄ productivity could be improved.

  16. Comparative Impacts of Scala Vestibuli Versus Scala Tympani Cochlear Implantation on Auditory Performances and Programming Parameters in Partially Ossified Cochleae.

    Science.gov (United States)

    Trudel, Mathieu; Côté, Mathieu; Philippon, Daniel; Simonyan, David; Villemure-Poliquin, Noémie; Bussières, Richard

    2018-07-01

    To compare scala vestibuli versus scala tympani cochlear implantation in terms of postoperative auditory performances and programming parameters in patients with severe scala tympani ossification. Retrospective case-control study. Tertiary referral center. One hundred three pediatric and adult patients who underwent cochlear implant surgery between 2000 and 2016. Three groups were formed: a scala vestibuli group, a scala tympani with ossification group, and a scala tympani without ossification group. Patients were matched based on their age, sex, duration of deafness, and side of implantation (ratio of 1:2:2). Postoperative evaluation of auditory performances and programming parameters following intensive functional rehabilitation program completion. Multimedia adaptive test (MAT), hearing in noise test (HINT SNR +10 dB, HINT SNR +5 dB, and HINT SNR +0 dB), impedances, neural response telemetry thresholds (NRT), neural response imaging thresholds (NRI), comfortable levels (C-levels), and threshold levels (T-levels) were compared between groups. Twenty-one patients underwent scala vestibuli cochlear implantation: 19 adults and two children. Auditory performances were similar between groups, although sentence recognition in a noisy environment was slightly higher in the scala vestibuli group. Impedance values were also higher in the scala vestibuli group, but all other programming parameters were similar between groups. We present the largest series of patients with scala vestibuli cochlear implantation. This approach provides at least comparable auditory performances without having any deleterious effects on programming parameters. This viable and useful insertion route might be the primary surgical alternative when facing partial cochlear ossification.

  17. Effects of the type of dietary fat at two levels of vitamin E in Wistar male rats during development and aging. III. Biochemical and morphometric parameters of the liver.

    Science.gov (United States)

    Porta, E A; Keopuhiwa, L; Joun, N S; Nitta, R T

    1981-03-01

    The purpose of this study was to explore in rats the possible influence of the type of dietary fat at two extreme levels of vitamin E on several biochemically determined hepatic changes and on a number of quantitatively analyzed structural and ultrastructural variations with age in hepatic cells. Six groups of weanling Wistar male rats were fed ad libitum isoenergetic diets containing similar amounts (15 g per 100 g diet) of saturated fat (coconut oil), unsaturated fat (safflower oil) or a combination of both at two levels of dl-alpha-tocopherol (2 or 200 mg per 100 g of diet). Determinations were performed in rats killed at 3, 6, 12, 18 and 24 months. Although in relation to age and irrespective of the type of diet, several of the biochemical parameters fluctuated with time, comparisons of the results between the youngest and oldest rats showed no changes in the levels of hepatic RNA, phospholipids, cholesterol, total tocopherols and total collagens, significant increases in DNA and triglycerides and a significant decrease in total protein. While the type of diet did not have in general significant influences on the levels of DNA, RNA, total protein and collagens, either the type of dietary fat and/or the levels of vitamin E had some definite effects on the levels of triglycerides, cholesterol, phospholipids and total tocopherols, as well as on the in vitro formation of malonaldehyde and on the eventual occurrence of in vivo lipoperoxidation (diene conjugation). These effects, however, varied in relation to the duration of the diverse dietary treatments. The morphologic studies indicated that all the livers had variable but generally moderate degrees of fatty changes (mainly due to triglyceride accumulation) which were attributed to the moderate obesity found in the rats. The mean nuclear and cell dimensions of hepatocytes, the number of binucleated hepatocytes, surface density of rough endoplasmic reticulum, numerical density of mitochondria and the fractional

  18. BISEN: Biochemical simulation environment

    NARCIS (Netherlands)

    Vanlier, J.; Wu, F.; Qi, F.; Vinnakota, K.C.; Han, Y.; Dash, R.K.; Yang, F.; Beard, D.A.

    2009-01-01

    The Biochemical Simulation Environment (BISEN) is a suite of tools for generating equations and associated computer programs for simulating biochemical systems in the MATLAB® computing environment. This is the first package that can generate appropriate systems of differential equations for

  19. Comparative Analysis of the Physicochemical Parameters of Breast Milk, Starter Infant Formulas and Commercial Cow Milks in Serbia

    Directory of Open Access Journals (Sweden)

    Sunarić Slavica

    2016-06-01

    Full Text Available Data on the physical properties of cow milk and infant formulas are important since they indicate the differences in physicochemical and rheological characteristics and compatibility with natural breast milk. This fact is important not only for quality control but also for the use of these commercial products as infant diet supplements or as complete breast milk substitutes. This study was undertaken to determine refractive index, surface tension, pH, electrical conductivity, viscosity and titratable acidity of the UHT cow milk, starter infant formulas and breast milk of Serbian mothers in order to compare commercial milk formulations with natural human milk. The paper also presents the measured data of some physical parameters of human milk about which there is little information in the literature. It has been also demonstrated how these parameters were changed by freezing and prolonged storage of breast milk.

  20. A hepcidina como parâmetro bioquímico na avaliação da anemia por deficiência de ferro Hepcidin as a biochemical parameter for the assessment of iron deficiency anemia

    Directory of Open Access Journals (Sweden)

    Andrea dos Reis Lemos

    2010-01-01

    parameter for assessment of iron deficiency anemia is reported in this review. A literature review was carried out in the databases PubMed and LILACS, between 2006-2010, assessing hepcidin as a parameter for the regulation of iron metabolism. During this period 35 studies were published in international journals and one study in a Brazilian journal. The production of hepcidin is homeostatically regulated by anemia and hypoxia. When oxygen supply is inadequate the level of hepcidin decreases. Therefore, more iron from the diet and from the stock of macrophages and hepatocytes becomes available. Hepcidin links to ferroportin, regulating iron release to plasma. When the hepcidin concentrations are low, the molecules of ferroportin are exposed on the plasmatic membrane and release iron. When hepcidin levels increase, hepcidin binds to molecules of ferroportin inducing its internalization and degradation and iron release gradually decreases. Apparently, development of diagnosis and therapy for anemia based on the parameter hepcidin may provide a more effective approach. Large-studies are needed to demonstrate the importance of hepcidin for the differential diagnosis of anemia, including sample protocols for analysis, with standards similar to those used in other biochemical evaluations, as well as the definition of cut-off points for the plasma and urinary expression of this peptide.

  1. SHORT COMMUNICATION: Status of Physical Fitness Index (PFI % and Anthropometric Parameters in Residential School Children Compared to Nonresidential School Children

    Directory of Open Access Journals (Sweden)

    Jyoti P Khodnapur

    2012-07-01

    Full Text Available Background: Physical fitness is the prime criterion for survival, to achieve any goal and to lead a healthy life. Effect of exercise to have a good physical fitness is well known since ancient Vedas. Physical fitness can be recorded by cardiopulmonary efficiency test like Physical Fitness Index (PFI % which is a powerful indicator of cardiopulmonary efficiency. Regular exercise increases PFI by increasing oxygen consumption. Residential school children are exposed to regular exercise and nutritious food under the guidance. Aims and Objectives: Our study is aimed to compare the physical fitness index status and anthropometric parameters in Residential Sainik (n=100 school children compared to Non-Residential (n=100 school children (aged between 12-16 years of Bijapur. Material and Methods: PFI was measured by Harvard Step Test [1]. TheAnthropometrical parameters like Height (cms, Weight (Kg, Body Surface Area (BSA in sq.mts, Body Mass Index (BMI in Kg/m2, Mid Arm Circumference (cms, Chest Circumference (cms and Abdominal Circumference (cms were recorded. Results: Mean score of PFI(%, Height(cms, Weight(Kg, BSA(sq.mts, BMI(Kg/m2, Mid Arm Circumference(cms, Chest Circumference (cms and Abdominal Circumference (cms were significantly higher (p=0.000 in Residential school children compared to Non Residential school children. In conclusion regular exercise and nutritious diet under the guidance increases the physical fitness and growth in growing children.

  2. Comparative evaluation of haematological parameters and erythrocyte membrane stability in pregnant and lactating goats in different seasons of tropical Savannah.

    Science.gov (United States)

    Habibu, B; Makun, H J; Yaqub, L S; Buhari, H U; Aluwong, T; Kawu, M U

    2017-09-01

    Haematological parameters and erythrocyte osmotic fragility (EOF) are commonly