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Sample records for biochem mol biol

  1. Aspectos biológicos y control de un gracilláriido (Gracillariidae: Lepidóptera en Caesalpinia spinosa (Mol. Kuntze (1898, en Cajamarca, Perú

    Directory of Open Access Journals (Sweden)

    Hipólito Murga Orrillo

    2016-01-01

    Full Text Available Se tuvo por objetivo determinar aspectos biológicos, identificar enemigos naturales, y establecer periodos oportunos de control de un gracillariido plaga de tara. Los aspectos biológicos y los enemigos naturales se evaluaron en campo y en laboratorio, los periodos oportunos de control se determinó por interacción hospedero - plaga. Los resultados, el huevo dura 7,8 días y mide 0,34x0,21mm; la larva presenta 4 instares y dura 35,7 días, variando de medidas entre 1 o y 4 o instar de 0,09 a 0,65 mm de diámetro de encéfalo ; la pupa dura 14,3 días y mide 6,7mmx1,1mm ; el adulto dura 7,5 días y mide 13,43 mm de expansión alar. Presenta enemigos naturales, familias Encyrtidae, Ichneumonidae, y géneros Chelonus sp., Chrysoperla sp., Hemerobius sp., Cicloneda sp . , Hipodamia sp., y Zelus spp. Las mejores épocas de control ocurren cuando la fenología de la tara est á entre R3.1 a R4 y R3.3 a R6.

  2. Avaliação do potencial biológico da Tabebuia aurea(Silva Manso como fonte de moléculas bioativas para atividade antimicrobiana, antiedematogênica e antirradicalar

    Directory of Open Access Journals (Sweden)

    R.F.E.P. SANTOS

    2015-01-01

    Full Text Available RESUMO As espécies da família Bignoniaceae e do gênero Tabebuia são amplamente utilizadas na medicina tradicional e possuem um forte potencial terapêutico. Diante disso, objetivou-se avaliar o potencial biológico da Tabebuia aurea, determinando a atividade antimicrobiana; por meio do método da microdiluição em caldo, para a determinação da Concentração Inibitória Mínima (CIM; antiedematogênica, pelo ensaio de edema de orelha induzido por capsaicina; e antirradicalar, frente ao radical DPPH. Os extratos etanólicos de T. aurea não evidenciaram citotoxicidade, exceto o extrato etanólico da flor nas concentrações > 0,5 mg mL-1. O extrato etanólico da flor foi ativo com ação bactericida frente a S. epidermidis (CIM de 0,06 mg mL-1 enquanto o extrato etanólico da folha foi moderadamente ativo frente a S. epidermidis (CIM: 0,25 mg mL-1 e S. aureus (CIM: 0,50 mg mL-1 sugerindo ação bacteriostática para ambas as linhagens. Os dois extratos apresentaram ação antiedematogênica, com inibição do edema de 40,50% pelo extrato etanólico da flor e de 41,73% pelo extrato da folha. T. aurea não apresentou atividade antirradicalar. Os resultados comprovam o perfil antibacteriano e antiedematogênico com ausência de citotoxidade pela T. aurea. Sugere-se a continuação dos testes com frações e substâncias isoladas das flores e folhas da referida espécie vegetal, bem como de experimentos in vivo, como forma de agregar evidências visando à busca de novos fitoterápicos.

  3. Aplicación de biomateriales de base biológica, moléculas bioactivas e ingeniería de tejidos en cirugía plástica periodontal: Una revisión Application of biological based biomaterials, bioactive molecules and tissue engineering in periodontal plastic surgery: A review

    Directory of Open Access Journals (Sweden)

    JC Durán Yaneth

    2012-12-01

    Full Text Available Objetivo: Describir, clasificar y discutir las indicaciones de los biomateriales de base biológica, moléculas bioactivas e ingeniería de tejidos que se están usando para el manejo de recesiones y aumento de encía en cirugía plástica periodontal. En esta revisión de la literatura, se utilizó una combinación de los términos de búsqueda específicos que consideraran los materiales en revisión, para el aumento de encía adherida, y el recubrimiento radicular. Materiales y Métodos: Se usaron las siguientes fuentes: Medline, Biblioteca Cochrane, y búsqueda manual de revistas específicas como el Journal of Periodontology, International Journal of Periodontics and Restorative Dentistry y Journal of Clinical Periodontology entre años 1985 y 2011. Se revisaron un total de 117 artículos y se seleccionaron 74 entre estudios clínicos controlados, estudios clínicos randomizados, reportes de casos y estudios en animales. Los artículos fueron revisados por los autores y aceptados por consenso para su discusión. Conclusiones: 1 Existe una serie de materiales que presentan gran potencial y podrían ser una alternativa viable a los injertos autógenos, pero se requiere más estudios a largo plazo. 2 Existe necesidad de estudios con la investigación de estos procedimientos en relación a resultados orientados a la estabilidad, seguridad y efectividad de los diferentes materiales existentes.Objective: To describe, classify and discuss the clinical applications of biologically based biomaterials, bioactive molecules and tissue engineering being utilized in gingival recession therapy and gingival augmentation procedures in plastic periodontal surgery. In this literature review, a combination of specific search key words were used, including materials being reviewed, indicated for gingival augmentation and root coverage procedures. Materials and Methods: The following sources were consulted: Medline, Cochrane Library and manual search of specific

  4. Nationalpark Mols Bjerge

    DEFF Research Database (Denmark)

    Buylaert, Jan-Pieter; Clausen, Ole Rønø; Højsager, Pia

    2017-01-01

    Nationalpark Mols Bjerge er geologisk set et rigtigt spændende område, hvor der erde bedste forudsætninger for at formidle historien om særligt istiden og dannelsenaf landskaberne i Danmark. Siden 2013 har Nationalparken finansieret en rækkeforskningsprojekter, der har tilvejebragt spændende ny...... viden: under selve MolsBjerge er der blevet opdaget to kalkrygge. Undersøgelser af istidens aflejringer harvist, at lagserien er ældre og mere komplekst opbygget, end vi hidtil har vidst og undersøgelseraf søsedimenter i to af nationalparkens søer har afdækket en spændendevegetationsudvikling, der...

  5. Armas biológicas

    OpenAIRE

    Bruhn Cuevas, Rosa María

    2016-01-01

    Abordamos el presente trabajo con el objetivo de realizar una revisión bibliográfica que nos permita elaborar un análisis del empleo de armas biológicas tanto en el pasado, es decir, una documentación de la utilización de éstas a lo largo de la historia, quiénes las utilizaban y el porqué de su utilización, dividido en varios periodos; como en el presente, si se siguen empleando en la actualidad, con especial mención de los protocolos que existen en cuanto a su regulación normativa a día de h...

  6. A first interpretation of the Mol 7C/l and Mol 7C/2 experiments

    International Nuclear Information System (INIS)

    Berthier, J.; Carluec, B.; Fortunato, M.; Lemmet, L.

    1983-01-01

    The interpretation of the two experiments MOL 7C/1 and MOL 7C/2 that has been performed at the CEA of Cadarache is presented here: one will find first a recall of the experimental conditions of the MOL experiments, then a short description of the codes that enable the interpretation and finally the results of this interpretation compared to the experimental results

  7. Mol - Research Division Report 1986 - 1

    International Nuclear Information System (INIS)

    Delbarre, J.; Assche, P. van

    1986-11-01

    This report covers the research activities carried out at the SCK-CEN, Mol from the middle of 1985 till mid 1986. It deals with materials science, metallurgy, ceramics, nuclear chemistry, chemical engineering, biology, nuclear meteorology and analytical chemistry. (MCB)

  8. Mol - Research Division Report 1986 - 2

    International Nuclear Information System (INIS)

    Delbarre, J.

    1987-03-01

    This report covers the research activities carried out at the SCK-CEN, Mol during the second semester of 1986. It deals with chemistry, chemical engineering, biology, nuclear metrology, analytical chemistry. (MCB)

  9. Mol - Research Division report 1987 - 2

    International Nuclear Information System (INIS)

    Delbarre, J.

    1988-03-01

    This report covers the research activities at the SCK-CEN, MOl, during the second semester of 1987. It deals with materials physics, nuclear physics, metallurgy, ceramics, nuclear chemistry, chemical engineering, biology, nuclear metrology and analytical chemistry. (MCB)

  10. Mol - Research Division report 1987 - 1

    International Nuclear Information System (INIS)

    Delbarre, J.

    1987-10-01

    This report covers the research activities at the SCK-CEN, Mol, during the first semester of 1987. It deals with material physics, nuclear physics, metallurgy, ceramics, nuclear chemistry, chemical engineering, biology, nuclear metrology and analytical chemistry. (MCB)

  11. Vojeslav Molè as Ekphrastic Poet

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    Robert Simonišek

    2017-07-01

    Full Text Available The paper analyses the ekphrastic poetry of the art historian Vojeslav Molè (1886–1973, who shaped Slovene art history and was in his youth a poet and essayist. Before he emigrated to Poland in 1926, Molè published two poetry collections, a few essays related to art history and some poems.. Formally, Molè’s poetry was marked by his interests in Antiquity and Symbolism, and his major themes were love, women, and, later in his career, an idealistic longing for youth. While in Rome he was fascinated by Tizian paintings Sacred and Profane Love, as well as Leonardo da Vinci’s Mona Lisa and Rafael’s Apollo and Marsyas, published in 1910 in his first book When Flowers Bloomed. During his stay in Rome he also wrote the essay Art and Nature, in which he discussed the relationships among visual art, literature and nature. Poems like Giorgione and Byzantine Madonna make reference to Venice and the monuments he visited during his travels. In terms of ekphrasis, Molè applies a classical approach to underline the beauty and importance of the focal artistic works. His ekphrastic perspective is rather similar to that of romantic poetry. During WW I Molè was captured as a prisoner and sent to Siberia, where he wrote the poems that were eventually collected in Tristia ex Siberia, published in 1920. Despite the fact that Molè was fascinated by Rilke’s poetry, which played important role in his artistic development, it is not possible to know whether he was influenced by that author’s most famous ekphrastic poem, Archaic Torso of Apollo. Overall, most of Molè’s ekphrastic poetry refers to much earlier works of art, with only a few exceptions. One of these is the poem The Sower, that could have been inspired by The Sower, an oil on canvas that was painted by Molè’s contemporary, the Slovene Impressionist Ivan Grohar.

  12. La esfera, el mol y la ciudad

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    Hernán Neira

    2010-06-01

    Full Text Available Apoyándonos en la noción de "esfera" de Slotedijk, analizamos la protección constituida por los moles. La libertad de elegir en el mol no tiene relación con la libertad individual propuesta por John Stuart Mill; ni con la anarco-individualista propuesta por Henry David Thoreau o incluso por Aldo Leopold en el elogio que éste realiza de la autonomía del pionero estadounidense. En la libertad de elegir en el mol han caducado todas las aventuras y las relaciones humanas se convierten en lo que Sloterdijk denomina "relaciones cristalizadas", bajo el proyecto del aburrimiento normativo poshistórico. La expresión económico-filosófica del mundo poshistórico es la idea de libertad de elegir, enunciada por Milton Friedman. El mol, actual Palacio de Cristal, es como una enciclopedia ilustrada, que exhibe, en orden y sin peligros, el conjunto, ya no del saber, sino de los bienes disponibles. Con ello, se modifica la condición humana.Based on the Sloterdijk's concept of sphere, we analyse the protection provided by the malls. Nevertheless, the freedom of choosing in a mall does not have any relation to liberty as it was proposed by John Stuart Mill, neither to the anarcho-individualist one proposed by Henry David Thoreau, nor even to that one proposed by Aldo Leopold, when he praises the authonomy of American pioneer. In the freedom process of choosing in the mall, all adventures perish and human relations become what Sloterdijk denominated "crystalised relations", under a post-historic normative boring project. The economic-philosophical expression of post-historic world is the idea of freedom of choosing, conceived by Milton Friedman. The mall, the current Crystal Palace, is like an enlightened encyclopaedia, which shows, in order and without dangers, the totality, no longer of knowledge, but of available goods. In this way, human condition has modified.

  13. Tiempo y edad biológica

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    Vargas, Esteban

    2013-04-01

    Full Text Available In this paper we attempt to think if it is possible to conceive age as a characteristic time of living organisms including man. In the paper we review several concepts of age coming from sciences such as chronobiology, gerontology, evolutionary biology and developmental biology, and trough all of them, we see that biological time is thought as time measured by a clock. We propose that age as biological time must be understood as a phase in the biological process, but depending on the complete cycle of the organism. In the case of human being, his age is determined also by what is “expected” about him. His age is then determined by biological and cultural aspects.En este trabajo intentamos meditar si es posible concebir la edad como un tiempo propio de los seres vivos incluyendo al hombre. En el trabajo revisamos diversos conceptos de edad obtenidos de diversas ciencias biológicas como la cronobiología, la gerontología, la biología evolutiva y la biología del desarrollo descubriendo que en ellas, en general, se sigue pensando el tiempo biológico como el tiempo que mide un reloj. Nosotros proponemos que la edad como tiempo biológico debe ser entendida como una fase del proceso biológico, pero en función del ciclo completo del organismo. En el caso del ser humano, su edad está determinada también por lo que se “espera” de él. Su edad está determinada entonces por aspectos biológicos y culturales.

  14. Spectral characterization of Quillaja saponaria (Mol.

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    T. Acuña

    2016-12-01

    Full Text Available This paper presents a spectral reflectance characterization of the specie Quillaja saponaria (Mol., endemic tree of Chile and valued by society due to its provision of several ecosystem services that gives to society and also for its high concentration of saponins in cortex widely used in the pharmacological industry. For spectral characterization a foliar spectral signatures protocol was designed which included standardized instrumental and environmental parameters. The spectral response of different individuals was measured to evaluate the spectral behaviour and degree of variability within species in the visible and near infrared ranges (VNIR; 400-990 nm with two hyperspectral sensors (ASD HH and camera PDF-65-V10E. The resulting spectral signatures obtained with ASD HH showed a variation less than 5% of reflectance in VNIR and lesser than that in the transition zone from red to near infrared (red-edge; 680-730 nm. Additionally, two distinctive spectral features were detected for the specie, the first is related to a fast increase of reflectance in bands 450-480 nm and the second, to a marked decrease in the 920-970 nm range associated with water absorption features. At branch level, these distinctive features are maintained but with a smaller magnitude of reflectance, which could indicate that they are useful characteristic spectral patterns that can eventually be used for monitoring the physical health state of the specie using remote sensing. On the other hand, we used a PDF-65 camera for study the plant vigour from different health states (healthy, ill, died with spectral vegetation index. The Plant Senescence Reflectance Index detected stress on leaves, and Triangular Vegetation Index allows for a gradually characterization of every state. This work provides the first spectral reference for one of the most important sclerophyll species of Chile.

  15. Ritmos biológicos y salud

    OpenAIRE

    Londoño Londoño, Hilda

    2011-01-01

    En el presente artículo se analizan algunos aspectos de la fisiología y del comportamiento de los seres vivos, particularmente del ser humano, supeditados a las condiciones del ambiente terrestre de donde surgieron y manejados por sus relojes biológicos. Estos mecanismos han sido estudiados principalmente a través de los ritmos circadianos, los cuales tienen una periodicidad de veinticuatro horas generalmente, y se encuentran involucrados en procesos tan diversos e importantes como la respira...

  16. CCQM-K90, formaldehyde in nitrogen, 2 μmol mol-1 Final report

    Science.gov (United States)

    Viallon, Joële; Flores, Edgar; Idrees, Faraz; Moussay, Philippe; Wielgosz, Robert Ian; Kim, D.; Kim, Y. D.; Lee, S.; Persijn, S.; Konopelko, L. A.; Kustikov, Y. A.; Malginov, A. V.; Chubchenko, I. K.; Klimov, A. Y.; Efremova, O. V.; Zhou, Z.; Possolo, A.; Shimosaka, T.; Brewer, P.; Macé, T.; Ferracci, Valerio; Brown, Richard J. C.; Aoki, Nobuyuki

    2017-01-01

    The CCQM-K90 comparison is designed to evaluate the level of comparability of national metrology institutes (NMI) or designated institutes (DI) measurement capabilities for formaldehyde in nitrogen at a nominal mole fraction of 2 μmol mol-1. The comparison was organised by the BIPM using a suite of gas mixtures prepared by a producer of specialty calibration gases. The BIPM assigned the formaldehyde mole fraction in the mixtures by comparison with primary mixtures generated dynamically by permeation coupled with continuous weighing in a magnetic suspension balance. The BIPM developed two dynamic sources of formaldehyde in nitrogen that provide two independent values of the formaldehyde mole fraction: the first one based on diffusion of trioxane followed by thermal conversion to formaldehyde, the second one based on permeation of formaldehyde from paraformaldehyde contained in a permeation tube. Two independent analytical methods, based on cavity ring down spectroscopy (CRDS) and Fourier transform infrared spectroscopy (FTIR) were used for the assignment procedure. Each participating institute was provided with one transfer standard and value assigned the formaldehyde mole fraction in the standard based on its own measurement capabilities. The stability of the formaldehyde mole fraction in transfer standards was deduced from repeated measurements performed at the BIPM before and after measurements performed at participating institutes. In addition, 5 control standards were kept at the BIPM for regular measurements during the course of the comparison. Temporal trends that approximately describe the linear decrease of the amount-of-substance fraction of formaldehyde in nitrogen in the transfer standards over time were estimated by two different mathematical treatments, the outcomes of which were proposed to participants. The two treatments also differed in the way measurement uncertainties arising from measurements performed at the BIPM were propagated to the

  17. El progreso biológico

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    Barahona, Ana

    1997-12-01

    Full Text Available Not available.La idea de progreso en Biología está ligada al concepto de scala naturae o la gran cadena del ser que se puede trazar hasta Aristóteles. Esta noción de que los organismos vivientes pueden clasificarse en una jerarquía de inferior a superior tomó formas nuevas en los siglos XVII y XVIII. En el siglo XVIII, naturalistas como Buffon, Bonnet y Robinet se habían preguntado si podíamos hablar de un patrón definido de desarrollo. En el siglo XIX, Lamarck postula la idea de que existe una progresión de los organismos menos avanzados a los organismos más avanzados. Posteriormente, la teoría de la evolución añade la dimensión temporal y de continuidad genética o histórica a la clasificación jerárquica de los seres vivos. Darwin pensaba que la selección natural traía como resultado el perfeccionamiento y que éste inevitablemente conduciría al progreso gradual de la organización. Herbert Spencer se convirtió en uno de los darwinistas más radicales del siglo XIX y principios del XX. Para Spencer la idea de progreso se aplicaba no solo al mundo biológico sino que era parte de una ley general, que dirigía lo simple hacia lo complejo, lo homogéneo a lo heterogéneo. Posteriormente, Julian Huxley, Theodosius Dobzhansky, Ernst Mayr, George Gaylord Simpson y Ledyard Stebbins —conocidos como los arquitectos de la Sintesis Moderna— comparten una visión progresionista de la evolución biológica. La noción de progreso evolutivo, en la actualidad, es sumamente controvertida, y se pueden distinguir básicamente tres diferentes perspectivas. La primera, defendida por Michael Ruse y David Hull entre otros, sostiene que no existe evidencia científica que nos lleve a aceptar cierta «direccionalidad» en la evolución biológica. Una segunda visión, defendida por Stephen J. Gould, sostiene que, en la medida en que reemplacemos el término de progreso (ya que éste es antropocéntrico por el de «noción operacional de

  18. Optimized MOL-PCR for Characterization of Microbial Pathogens.

    Science.gov (United States)

    Wuyts, Véronique; Roosens, Nancy H C; Bertrand, Sophie; Marchal, Kathleen; De Keersmaecker, Sigrid C J

    2016-01-06

    Characterization of microbial pathogens is necessary for surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation-PCR (MOL-PCR) optimized for characterization of microbial pathogens. With MOL-PCR, different types of markers, like unique sequences, single-nucleotide polymorphisms (SNPs) and indels, can be simultaneously analyzed in one assay. This assay consists of a multiplex ligation for detection of the markers, a singleplex PCR for signal amplification, and hybridization to MagPlex-TAG beads for readout on a Luminex platform after fluorescent staining. The current protocol describes the MOL-PCR, as well as methods for DNA isolation, probe design, and data interpretation and it is based on an optimized MOL-PCR assay for subtyping of Salmonella Typhimurium. Copyright © 2016 John Wiley & Sons, Inc.

  19. Simulering af ændret publikumsbelastning i Mols Bjerge

    DEFF Research Database (Denmark)

    Skov-Petersen, Hans; Foged, Mette

    2010-01-01

    Det kan være svært at forudse, hvad effekten af ændringer i adgangsforholdene til et naturområde vil blive. Dette Videnblad beskriver, hvordan en agent-baseret simuleringsmodel kan anvendes til vurdering af fremtiden ved flytning af en parkeringsplads ved trehøje i Mols Bjerge.......Det kan være svært at forudse, hvad effekten af ændringer i adgangsforholdene til et naturområde vil blive. Dette Videnblad beskriver, hvordan en agent-baseret simuleringsmodel kan anvendes til vurdering af fremtiden ved flytning af en parkeringsplads ved trehøje i Mols Bjerge....

  20. Geomechanical research in the underground laboratory at Mol

    International Nuclear Information System (INIS)

    Neerdael, B.; De Bruyn, D.

    1989-01-01

    Within the European Community programme on Management and Storage of radioactive waste, investigations about the rock mechanical behaviour of natural clays are carried out including laboratory and in-situ tests under ambient and elevated temperature conditions performed in the scope of the underground laboratory at Mol. Items related to stress field and clay fracturation are developed

  1. Mol weight.pdf | chemistry | resources | sci ed | initiat | Indian ...

    Indian Academy of Sciences (India)

    Home; initiat; sci ed; resources; chemistry; Mol weight.pdf. 404! error. The page your are looking for can not be found! Please check the link or use the navigation bar at the top. YouTube; Twitter; Facebook; Blog. Academy News. IAS Logo. Theory Of Evolution. Posted on 23 January 2018. Joint Statement by the Three ...

  2. Mol2vec: Unsupervised Machine Learning Approach with Chemical Intuition.

    Science.gov (United States)

    Jaeger, Sabrina; Fulle, Simone; Turk, Samo

    2018-01-22

    Inspired by natural language processing techniques, we here introduce Mol2vec, which is an unsupervised machine learning approach to learn vector representations of molecular substructures. Like the Word2vec models, where vectors of closely related words are in close proximity in the vector space, Mol2vec learns vector representations of molecular substructures that point in similar directions for chemically related substructures. Compounds can finally be encoded as vectors by summing the vectors of the individual substructures and, for instance, be fed into supervised machine learning approaches to predict compound properties. The underlying substructure vector embeddings are obtained by training an unsupervised machine learning approach on a so-called corpus of compounds that consists of all available chemical matter. The resulting Mol2vec model is pretrained once, yields dense vector representations, and overcomes drawbacks of common compound feature representations such as sparseness and bit collisions. The prediction capabilities are demonstrated on several compound property and bioactivity data sets and compared with results obtained for Morgan fingerprints as a reference compound representation. Mol2vec can be easily combined with ProtVec, which employs the same Word2vec concept on protein sequences, resulting in a proteochemometric approach that is alignment-independent and thus can also be easily used for proteins with low sequence similarities.

  3. Development of a standard reference material containing 22 chlorinated hydrocarbon gases at 1 μmol/mol in nitrogen.

    Science.gov (United States)

    Li, Ning; Du, Jian; Yang, Jing; Fan, Qiang; Tian, Wen

    2017-11-01

    A gas standard mixture containing 22 chlorinated hydrocarbons in high purity nitrogen was prepared using a two-step weighing method and a gasifying apparatus developed in-house. The concentration of each component was determined using a gas chromatograph with flame ionization detection (GC/FID). Linear regression analysis of every component was performed using the gas standard mixture with concentrations ranging from 1 to 10 μmol/mol, showing the complete gasification of volatile organic compound (VOCs) species in a selected cylinder. Repeatability was also examined to ensure the reliability of the preparation method. In addition, no significant difference was observed between domestic treated and imported treated cylinders, which were conducive to reduction of the cost of raw materials. Moreover, the results of stability testing at different pressures and long-term stability tests indicated that the gas standard at 1 μmol/mol level with relative expanded uncertainties of 5% was stable above 2 MPa for a minimum of 12 months. Finally, a quantity comparison was conducted between the gas standard and a commercial gas standard from Scott Specialty Gases (now Air Liquide America Specialty Gases). The excellent agreement of every species suggested the favorable accuracy of our gas standard. Therefore, this reference material can be applied to routine observation of VOCs and for other purposes.

  4. Functional role of a high mol mass protein complex in the sea urchin yolk granule.

    Science.gov (United States)

    Perera, Aruni; Davis, Philip; Robinson, John J

    2004-04-01

    We have investigated the biochemical and functional characteristics of the major protein constituents of the yolk granule organelle present in sea urchin eggs and embryos. Compositional analysis, using sodium dodecyl sulfate polyacrylamide gel electrophoresis, revealed distinctly different polypeptide patterns under reducing and non-reducing conditions. In the presence of reducing agent, a 240 kDa species dissociated into polypeptides of apparent mol mass 160, 120 and 90 k. The relatedness of these polypeptides to the 240 kDa species was demonstrated in protein gel blot and peptide mapping analyses. The profile of yolk granule polypeptides was dynamic during embryonic development with the disappearance of the 160 kDa species and the coincidental appearance of lower mol mass polypeptides. However, the 240 kDa complex was detected even after the disappearance of the 160 kDa polypeptide. The 240 kDa complex was released from yolk granules in the absence of calcium and the purified species was shown to bind liposomes in a calcium-dependent manner. In addition, the 240 kDa complex possessed a calcium-dependent, liposome aggregating activity. The 240 kDa species could also induce the aggregation of yolk granules, previously denuded of the complex following treatment with either ethylenediaminetetraacetic acid or trypsin. Collectively, these results demonstrate the dynamic characteristics of the yolk granule 240 kDa protein complex and offer insights into a possible functional role.

  5. Multi criteria decision analysis on a waste repository in Mol

    International Nuclear Information System (INIS)

    Carle, B.

    2005-01-01

    In Belgium, the management of radioactive waste is taken care of by ONDRAF/NIRAS, the Belgian Agency for Radioactive Waste and Enriched Fissile Materials. Local partnerships with stakeholders from municipalities in existing nuclear zones were setup to facilitate the dialogue between the repository designers and the local community. Since the establishment of the partnership in Mol, MONA in February 2000, all aspects of a possible near-surface or a deep geological repository are discussed in 4 working groups by around 50 volunteer members. The outcome of the discussions in the partnership can be a shared project, supported by both local stakeholders and ONDRAF/NIRAS, in which the specifications and the conditions needed for establishing a repository in Mol are elaborated. MONA asked the Decision Strategy Research Department of SCK-CEN to organise a Multi Criteria Analysis (MCA) in the context of the deciding between a surface and a deep repository for low level radioactive waste. The objective of the multi criteria analysis is to support a number of representatives of the various working groups within MONA in their selection between two acceptable options for a repository of low level radioactive waste on the territory of Mol. The options are the surface repository developed by the working groups of MONA, and a deep repository in the clay layers underneath the nuclear site of Mol. This study should facilitate the selection between both options, or in case this appears to be difficult, at least to get a well-structured overview of all factors (criteria) of importance to the judgement, and to get insight into the degree in which the various criteria contribute to the selection

  6. Dreta i esquerra: les molècules quirals

    OpenAIRE

    Solà i Puig, Miquel; Duran i Portas, Miquel

    2007-01-01

    Molècules de dretes i d'esquerres? Una història científica amb contribució de la UdG: investigadors de l'Institut de Química Computacional de la UdG van participar en una recerca que amb posterioritat va ser premiada amb el Nobel de química del 2001

  7. Crystal and mol-ecular structure of aflatrem.

    Science.gov (United States)

    Lenta, Bruno N; Ngatchou, Jules; Kenfack, Patrice T; Neumann, Beate; Stammler, Hans-Georg; Sewald, Norbert

    2015-11-01

    The crystal structure of the title compound, C32H39NO4, confirms the absolute configuration of the seven chiral centres in the mol-ecule. The molecule has a 1,1-dimethylprop-2-enyl substituent on the indole nucleus and this nucleus shares one edge with the five-membered ring which is, in turn, connected to a sequence of three edge-shared fused rings. The skeleton is completed by the 7,7-trimethyl-6,8-dioxabi-cyclo-[3.2.1]oct-3-en-2-one group connected to the terminal cyclohexene ring. The two cyclohexane rings adopt chair and half-chair conformations, while in the dioxabi-cyclo-[3.2.1]oct-3-en-2-one unit, the six-membered ring has a half-chair conformation. The indole system of the mol-ecule exhibits a tilt of 2.02 (1)° between its two rings. In the crystal, O-H⋯O hydrogen bonds connect mol-ecules into chains along [010]. Weak N-H⋯π inter-actions connect these chains, forming sheets parallel to (10-1).

  8. Structural evolution of plasma-sprayed nanoscale 3 mol% and 5 mol% yttria-stabilized zirconia coatings during sintering

    Science.gov (United States)

    Zhao, Yan; Gao, Yang

    2017-12-01

    The microstructure of plasma-sprayed nanostructured yttria-stabilized zirconia (YSZ) coatings may change during high-temperature exposure, which would influence the coating performance and service lifetime. In this study, the phase structure and the microstructural evolution of 3YSZ (zirconia-3 mol% yttria) and 5YSZ (zirconia-5 mol% yttria) nanostructured coatings were investigated by means of sintering at 1400 °C for 50-100 h. The microhardness, elastic moduli, and thermal shock cycles of the 3YSZ and 5YSZ nanostructured coatings were also investigated. The results showed that the redistribution of yttrium ions at 1400 °C caused the continuous increase of monoclinic-phase zirconia, but no obvious inter-splat cracking formed at the cross-sections, even after 100 h. Large voids appeared around the nanoporous zone because of the sintering of nanoscale granules upon high-temperature exposure. The microhardness and elastic moduli of the nanostructured coatings first increased and then decreased with increasing sintering times. The growth rate of the nanograins in the 3YSZ coating was lower than that in 5YSZ, which slowed the changes in 3YSZ coating porosity during sintering. Although the 3YSZ coating was prone to monoclinic phase transition, the experimental results showed that the thermal shock resistance of the 3YSZ coating was better than that of the 5YSZ coating.

  9. Gold Rush in Mol at the 15th ASCERI Atomiade

    CERN Multimedia

    Chris Haen, Konrad Jende & Rachel Bray, Atomiade organizers and athletes for the CERN team

    2015-01-01

    What initially began as a football tournament between German institutes involved in nuclear research has developed into ASCERI (Association of the Sports Communities of the European Research Institutes), which aims to contribute to a united Europe through regular sport meetings, bringing together members of public research institutes at the European level. The members come from over 40 research institutes (see here) spanning 16 countries.     CERN team going for Gold at the summer Atomiade!   One of the main sport events organised by ASCERI, the 15th Atomiade was held from 5 to 8 June 2015 (organisational committee JRC Geel) in Mol, in the Kempen region of Belgium. CERN participated by sending 40 athletes from 15 countries to compete in a variety of disciplines: Athletics, Basketball, Cycling, Mountain Bike Race, Canoeing/Kayak, S.U.P., Swimming, Table Tennis, Tennis, Triathlon, Volleyball. The 1200 athletes from 36 European research institutes were housed in th...

  10. Anatomia foliar de Maytenus Mol. emend Mol. (Celastraceae, ocorrente no Estado do Rio de Janeiro, Brasil Leaf anatomy of the Maytenus Mol. emend Mol. (Celastraceae in Rio de Janeiro State, Brazil

    Directory of Open Access Journals (Sweden)

    Ana Joffily

    2005-09-01

    Full Text Available Este trabalho aborda o estudo anatômico de cinco espécies do gênero Maytenus Mol. emend. Mol. (Celastraceae. O gênero é o maior da família, e está representado no Brasil por aproximadamente 80 espécies. Maytenus é um gênero polifilético, necessitando de trabalhos adicionais para nova circunscrição. Maytenus ardisiaefolia Reiss., M. brasiliensis Mart., M. cestrifolia Reiss., M. communis Reiss. e M. obtusifolia Mart. são de difícil identificação, devido à semelhança dos verticilos reprodutivos, e pela variação na forma e no tamanho das folhas. As estruturas anatômicas observadas nas diversas partes da folha mostraram-se muito semelhantes nas espécies estudadas, sendo de valor taxonômico e de provável importância filogenética para o gênero Maytenus. A organização da epiderme e estratos subepidérmicos em M. obtusifolia demonstrou ser caráter diagnóstico importante na comparação com M. ardisiaefolia, e a ocorrência de cristais aciculares nas células epidérmicas de M. communis e de M. ardisiaefolia, que as separam de M. cestrifolia, que não apresenta cristais, apontaram a anatomia como importante ferramenta a ser explorada nos estudos taxonômicos das demais espécies do gênero. A observação e descrição das verrugas suberosas na epiderme da lâmina foliar poderá contribuir para a taxonomia das Celastraceae.This paper describes anatomical approaches to study the Genus Maytenus Mol. Emnd. Mol. (Celastraceae. The genus is the largest in the family Celastraceae, and in Brazil is represented by approximately 80 species. Maytenus is a polyphyletic genus and additional research is required to better understand its taxonomy. Maytenus ardisiaefolia Reiss., M. brasiliensis Mart., M. cestrifolia Reiss., M. communis Reiss. and M. obtusifolia Mart. are difficult to identify because of similarities in their reproductive structures and variations in the size and shape of their leaves. The anatomical structures observed

  11. Concepciones de los profesores acerca del concepto mol

    Directory of Open Access Journals (Sweden)

    Saida Matute

    2015-08-01

    Full Text Available ResumenEste estudio analiza las concepciones del concepto de mol que tienen los docentes que administran la asignatura de química a nivel de bachillerato del municipio Bruzual-Yaracuy (periodo escolar 2012-2013. La muestra está representada por sesenta docentes que respondieron la encuesta. Los resultados indican que la mayoría de ellos tienen buena información en relación con el átomo y masa atómica de un elemento. En lo que se refiere al mol, como se atribuye su significado a la unidad individual de masa, de porción de sustancia y de número de partículas (número de Avogadro, se detecta que no tienen conocimiento de la definición que le dio la Iupac, lo que induce a pensar su desconocimiento acerca de dicha definición. AbstractThis study analyzes the conceptions of the term “mole” according to Chemistry teachers in Secondary school in the municipality of Yaracuy Bruzual (School year 2012-2013. The sample is represented by sixty teachers who responded to the survey. The results indicate that most of them have good information regarding atom and atomic weight of an element. With regard to the term “mol”, it was determined that they lack of knowledge on the definition given by Iupac, since its meaning is related to the individual unit of mass, portion of substance and number of particles (Avogadro’s number.

  12. Biochemical characterization of the phospholipase A2 purified from the venom of the Mexican beaded lizard (Heloderma horridum horridum Wiegmann).

    Science.gov (United States)

    Sosa, B P; Alagón, A C; Martin, B M; Possani, L D

    1986-05-20

    A phospholipase A2 was isolated from the venom of the mexican beaded lizard (Heloderma horridum horridum) by phenyl-Sepharose chromatography followed by Sephadex G-75 gel filtration and two additional steps on ion exchange resins (DE-32 cellulose). The affinity chromatographic method (PC-Sepharose 4B) reported for the isolation of other phospholipases [Rock, Ch. O., & Snyder, F. (1975) J. Biol. Chem. 250, 2564-2566; King, T. P., Alagon, A. C., Kwan, J., Sobotka, A. K., & Lichteinstein, L. M. (1983) Mol. Immunol. 20, 297-308; King, T. P., Kochoumian, L., & Joslyn, A. (1984) Arch. Biochem. Biophys. 230, 1-12] was uneffective for the separation of this enzyme. The monomeric form of the Heloderma phospholipase has an apparent Mr of 18 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 19 060 as calculated from amino acid analysis. It also contains on the order of 7% carbohydrates per mole of enzyme. The N-terminal amino acid sequence was shown to be very different from that of phospholipases isolated from mammalian pancreas and crotalids and elapids snake venoms. The first 39 amino acid residues at the N-terminal region have 56% homology with bee venom phospholipase but differ from the bee phospholipase in that its isoelectric point is acidic (pI = 4.5), instead of basic, and it has approximately 50 amino acid residues more in the molecule. The specificity of the enzyme is mainly A2 type with possible residual B-type activity. The enzymatic activity is Ca2+-dependent. Half-cystine alignment of the Heloderma phospholipase sequence with those of other known phospholipases shows the lack of an octadecapeptide at the N-terminal region, the existence of an extra hexapeptide at positions 42-47, and an exact correspondence of Heloderma Gly-12, Gly-14, His-36, and Asp-37 with Gly-30, Gly-32, His-48, and Asp-49 from other phospholipases shown to be important for Ca2+ binding (( Dijkstra, B. W., Drenth, J., Kalk, K. H., & Vandermaalen, P. J. (1978) J. Mol. Biol

  13. Biolingüística y capacidad humana

    Directory of Open Access Journals (Sweden)

    Chomsky Noam Abraham

    2006-06-01

    Full Text Available Este artículo presenta de manera sucinta el enfoque llamado 'biolingüístico' que empezó a desarrollarse en los primeros años de la posguerra, teniendo en cuenta los avances de la biología y las matemáticas. Se pasa revista a algunos postulados de la neurociencia (i.e. ciencia cognitiva en lo concerniente a la 'facultad del lenguaje', así como a los dos últimos modelos de la GG: el modelo de 'Principios y Parámetros' (P&P y el modelo 'Minimalista'. Estos últimos buscan responder las cuestiones fundamentales de la biología del lenguaje, su naturaleza, uso y evolución. Desde este punto de vista, se trata de caracterizar el equipamiento biológico gracias al cual los seres humanos adquieren una gramática. Así las cosas, se considera el equipamiento biológico como una función que asocia experiencia y gramática.

  14. Evaluation of biol`s effect on fourteen accession of native potato (Solanum ssp. at the experimental station kallutaca

    Directory of Open Access Journals (Sweden)

    Condori-Mamani Pastor

    2017-05-01

    Full Text Available In Bolivia the productive potato regions, you characterize for an extensive agriculture in blankets (of traditional form, of car subsistence and not much diversified, the little properties for the cultivation of potato possess the greater part of the productive, and they locate at little plots of land that annually they go rotating. The present research work has the objective to make a comparison and to characterize fourteen accessions of native potato of germoplasma's collection, of Agronomic Ingeniería's race, (UPEA. The study carried completion at Kallutaca's experiment station itself, becoming established on the basis of a Design Bloques Complete at random (DBCA with three repetitions each accession, the second factor is diligent biol's dose, considered himself like a stimulant compound fito, for cultivations, where increments in the capability of photosynthesis and the production allow principal. Whose moral values place between 2.0 2.6% themselves (% in dry material and that they yielded 3.3 %'s concentration of total N to 3.7%. The analysis of microcomputer Hierro's (132 ppm, Manganeso's, Cobre's and Zinc's presence with 12.1 and 6 ppm detected nutrient in the obtained effluent. Accessions Pole, Sacampaya, Sani imilla, Janq'o, collide, Runic Bola, Q'aysalla and Laramq'aysa had bigger development, the same the remainders have bigger performances they did not have the same incidence. This answer is due to a positive effect of the biol on the performance of tubers in accordance with dose of diligent biol the accessions of native potato where 1 with the application of dose of 25 % of Biol obtained to 10 t.h-1 an equivalent yield.

  15. ExoMol line list - XXI. Nitric Oxide (NO)

    Science.gov (United States)

    Wong, Andy; Yurchenko, Sergei N.; Bernath, Peter; Müller, Holger S. P.; McConkey, Stephanie; Tennyson, Jonathan

    2017-09-01

    Line lists for the X 2Π electronic ground state for the parent isotopologue of nitric oxide (14N16O) and five other major isotopologues (14N17O, 14N18O, 15N16O, 15N17O and 15N18O) are presented. The line lists are constructed using empirical energy levels (and line positions) and high-level ab initio intensities. The energy levels were obtained using a combination of two approaches, from an effective Hamiltonian and from solving the rovibronic Schrödinger equation variationally. The effective Hamiltonian model was obtained through a fit to the experimental line positions of NO available in the literature for all six isotopologues using the programs spfit and spcat. The variational model was built through a least squares fit of the ab initio potential and spin-orbit curves to the experimentally derived energies and experimental line positions of the main isotopologue only using the duo program. The ab initio potential energy, spin-orbit and dipole moment curves (PEC, SOC and DMC) are computed using high-level ab initio methods and the marvel method is used to obtain energies of NO from experimental transition frequencies. The line lists are constructed for each isotopologue based on the use of the most accurate energy levels and the ab initio DMC. Each line list covers a wavenumber range from 0 to 40 000 cm-1 with approximately 22 000 rovibronic states and 2.3-2.6 million transitions extending to Jmax = 184.5 and vmax = 51. Partition functions are also calculated up to a temperature of 5000 K. The calculated absorption line intensities at 296 K using these line lists show excellent agreement with those included in the HITRAN and HITEMP data bases. The computed NO line lists are the most comprehensive to date, covering a wider wavenumber and temperature range compared to both the HITRAN and HITEMP data bases. These line lists are also more accurate than those used in HITEMP. The full line lists are available from the CDS http://cdsarc.u-strasbg.fr and ExoMol www

  16. Control biológico de ácaros Tetranychidae

    Directory of Open Access Journals (Sweden)

    Acosta Gómez Alfredo

    1992-12-01

    Full Text Available En este articulo, se presenta información publicada e inédita y, también, se realiza una revisión bibliográfica exhaustiva de los enemigos naturales de T. cinnabarinus y T. urticae, tanto a nivel mundial, como a nivel nacional, con el propósito de despertar el interés e informar sobre las bondades del control
    biológico de los ácaros Tetranychidae y, en los casos donde es posible, se proporciona alguna información correspondiente a su efectividad. Posteriormente, se plantea una discusión resumida y se sugieren algunas propuestas de comportamiento estratégico o de utilidad potencial que podrían ser adoptadas por los profesionales comprometidos o
    responsables de los cultivos de flores para promover
    la ampliación del uso de enemigos naturales de los ácaros y utilizar, entre otros, métodos o estrategias biológicas, culturales o flsicas, que ayuden a disminuir la frecuencia del uso de agroquímicos y, así, conservar un ambiente menos contaminado y menos perjudicial para la humanidad del futuro.

  17. Las armas biológicas. Perspectivas de futuro

    OpenAIRE

    Nieva Machín

    2014-01-01

    La valoración de bajo riesgo de sufrir un ataque con armas biológicas realizada por la estrategia nacional de seguridad resulta errónea ya que España como miembro de la OTAN debería recoger en su estrategia nacional de seguridad del año 2013 esta amenaza ya considerada como tal por la OTAN en el año 2002. Para una valoración efectiva del nivel de esta amenaza resulta preciso considerar que esta ha trascendido el ámbito militar. En el presente artículo se presentará la vulnerabilidad de España...

  18. GridMol: a grid application for molecular modeling and visualization

    Science.gov (United States)

    Sun, Yanhua; Shen, Bin; Lu, Zhonghua; Jin, Zhong; Chi, Xuebin

    2008-02-01

    In this paper we present GridMol, an extensible tool for building a high performance computational chemistry platform in the grid environment. GridMol provides computational chemists one-stop service for molecular modeling, scientific computing and molecular information visualization. GridMol is not only a visualization and modeling tool but also simplifies control of remote Grid software that can access high performance computing resources. GridMol has been successfully integrated into China National Grid, the most powerful Chinese Grid Computing platform. In Section "Grid computing" of this paper, a computing example is given to show the availability and efficiency of GridMol. GridMol is coded using Java and Java3D for portability and cross-platform compatibility (Windows, Linux, MacOS X and UNIX). GridMol can run not only as a stand-alone application, but also as an applet through web browsers. In this paper, we will present the techniques for molecular visualization, molecular modeling and grid computing. GridMol is available free of charge under the GNU Public License (GPL) from our website: http://www.sccas.cn/ syh/GridMol/index.html.

  19. Las β-(1®3-glucanas: moléculas inmunomoduladoras contaminantes de productos farmacéuticos

    Directory of Open Access Journals (Sweden)

    Zenia Pardo Ruiz

    Full Text Available Se realizó una búsqueda bibliográfica utilizando la base de datos Pubmed con énfasis en los artículos publicados en la última década. Como descriptores se utilizaron los siguientes: glucans, glucans recognition, glucans biological activitiy, glucans pharmaceuticals. Con la información disponible se realizó un análisis de los principales aspectos relacionados con el tema, que se exponen en el presente trabajo. Las b-(1®3-glucanas son polímeros de glucosa que se encuentran mayoritariamente en la pared celular de hongos, levaduras y plantas. Se consideran patrones moleculares asociados a patógenos y son reconocidas por varios receptores, siendo la dectina-1 el principal receptor de reconocimiento de estas estructuras. Sus propiedades inmunomoduladoras han sido informadas por varios autores. Se ha demostrado que potencian y sinergizan la acción de ligandos de Toll like receptors sobre la liberación de citoquinas proinflamatorias, aunque también han mostrado un perfil antiinflamatorio, cuestión que depende en gran medida de sus características estructurales. Las b-(1®3-glucanas son contaminantes importantes provenientes de los filtros de acetato de celulosa que se utilizan en la clarificación de parenterales hemoderivados, por tanto, es necesario estudiar las consecuencias de la presencia de estas moléculas inmunomoduladoras en inyectables. En esta revisión se resumen aspectos relacionados con el reconocimiento y actividad biológica de las b-(1®3-glucanas y se profundiza en estudios relacionados con su presencia en hemoderivados como principal contaminante. Finalmente se destaca la utilidad de la Prueba de Activación de Monocitos en la detección de las b-(1®3-glucanas en parenterales.

  20. An Introduction to Using the Method of Levels (MOL) Therapy to Work with People Experiencing Psychosis.

    Science.gov (United States)

    Tai, Sara J

    2016-01-01

    This paper provides a basic introduction to using method of levels (MOL) therapy with people experiencing psychosis. As MOL is a direct application of perceptual control theory (PCT), a brief overview of the three main theoretical principles of this theory--control, conflict, and reorganization will be outlined in relation to understanding psychosis. In particular, how these principles form the basis of problem conceptualisation and determine what an MOL therapist is required to do during therapy will be illustrated. A practical description of MOL will be given, using case examples and short excerpts of therapeutic interactions. Some direct contrasts will also be made with cognitive behaviour therapy for psychosis (CBTp) and psychodynamic approaches (PA) in order to help illustrate the theory and practice of MOL.

  1. HackaMol: An Object-Oriented Modern Perl Library for Molecular Hacking on Multiple Scales.

    Science.gov (United States)

    Riccardi, Demian; Parks, Jerry M; Johs, Alexander; Smith, Jeremy C

    2015-04-27

    HackaMol is an open source, object-oriented toolkit written in Modern Perl that organizes atoms within molecules and provides chemically intuitive attributes and methods. The library consists of two components: HackaMol, the core that contains classes for storing and manipulating molecular information, and HackaMol::X, the extensions that use the core. The core is well-tested, well-documented, and easy to install across computational platforms. The goal of the extensions is to provide a more flexible space for researchers to develop and share new methods. In this application note, we provide a description of the core classes and two extensions: HackaMol::X::Calculator, an abstract calculator that uses code references to generalize interfaces with external programs, and HackaMol::X::Vina, a structured class that provides an interface with the AutoDock Vina docking program.

  2. Las armas biológicas. Perspectivas de futuro

    Directory of Open Access Journals (Sweden)

    Nieva Machín

    2014-05-01

    Full Text Available La valoración de bajo riesgo de sufrir un ataque con armas biológicas realizada por la estrategia nacional de seguridad resulta errónea ya que España como miembro de la OTAN debería recoger en su estrategia nacional de seguridad del año 2013 esta amenaza ya considerada como tal por la OTAN en el año 2002. Para una valoración efectiva del nivel de esta amenaza resulta preciso considerar que esta ha trascendido el ámbito militar. En el presente artículo se presentará la vulnerabilidad de España derivaba de las políticas y protocolos de seguridad de carácter reactivo y no preventivo establecidas en España así como aspectos mutidimensionales que requieren un replanteamiento y mejoras acordes a una valoración de riesgo más precisa.

  3. Ionic conductivity ageing behaviour of 10 mol.% Sc2O3–1 mol.% CeO2–ZrO2 ceramics

    DEFF Research Database (Denmark)

    Omar, Shobit; Bonanos, Nikolaos

    2010-01-01

    The long-term ionic conductivity behaviour of samples of zirconia co-doped with 10 mol.% of Sc2O3 and 1 mol.% CeO2 is evaluated in oxidizing and reducing atmospheres at 600 °C. After 3,000 h, the sample kept in reducing atmospheres exhibits 20% loss in the ionic conductivity, while the sample kept...... in air shows 6% degradation. No phase transitions were observed in the samples after both the ageing studies. The main contribution towards the loss in the ionic conductivity of the sample kept in air comes from grain boundaries; however, for the sample aged in reducing conditions, both grain and grain...

  4. Microencapsulación de compuestos de actividad biológica

    Directory of Open Access Journals (Sweden)

    Mary Lopretti

    2011-05-01

    Full Text Available La microencapsulación de compuestos de actividad biológica (ADN, fármacos, proteínas, probióticos, enzimas, etc., desde el punto de vista tecnológico, podría definirse como el proceso de recubrimiento de dichos compuestos, bajo la forma de moléculas, partículas sólidas o glóbulos líquidos, con materiales de distinta naturaleza, para dar lugar a partículas de tamaño micrométrico. Uno de los polímeros naturales más utilizados para la producción de microesferas es el quitosano (?-1,4- glucosamina. Diversos métodos han sido propuestos para la producción de microcápsulas, divididos en tres grupos: procesos físicos, procesos químicos y procesos físico-químicos. En el presente trabajo se ensayaron distintas metodologías para la producción de microesferas y microcápsulas de quitosano. Según la metodología empleada se obtuvieron distintos tipos de esferas en lo que respecta a tamaño y densidad principalmente. Las micropartículas obtenidas se evaluaron mediante microscopía óptica, electrónica de barrido, así como se realizó la evaluación de su estabilidad y liberación del agente encapsulado.  AbstractThe microencapsulation process of agents with biological activity (such as DNA, pharmaceuticals, proteins, probiotics, enzymes, etc., from the technological view, could be defined as the coating process of those agents, under a molecular form, solid particles, or liquid globules, with materials of different nature, that gives particles particles of micrometric size. One of the most used natural polymers for the production of microspheres is chitosan (?-1,4-glucosamine. Various methods have been proposed for the production of microcapsules, divided into three groups: physical processes, chemical processes, and physico-chemical processes. In this work, it were assayed different methodologies for the production of chitosan microspheres and microcapsules. Depending on the methodology used, were obtained differente types of

  5. Terapia com agentes biológicos na criança e no adolescente

    OpenAIRE

    Suehiro,Ricardo Maisse; Aikawa,Nádia Emi; Carvalho,Jozélio Freire de; Silva,Clovis Artur A. da

    2010-01-01

    OBJETIVO: Revisar os mecanismos fisiopatológicos e novos alvos terapêuticos, os agentes biológicos disponíveis, principais indicações e a evidência científica atual para o uso de terapias biológicas na população pediátrica. FONTES DE DADOS: Pesquisa na base de dados Medline e SciELO, nas línguas inglesa e portuguesa, entre 2000 e 2009. As palavras-chave usadas foram "agentes biológicos", "crianças" e "adolescentes". SÍNTESE DOS DADOS: Os agentes biológicos são uma importante opção terapêutica...

  6. Hydrocarbon gas standards at the pmol/mol level to support ambient atmospheric measurements.

    Science.gov (United States)

    Rhoderick, George C; Duewer, David L; Ning, Li; DeSirant, Kathryn

    2010-02-01

    Studies of climate change increasingly recognize the diverse influences exerted by hydrocarbons in the atmosphere, including roles in particulates and ozone formation. Measurements of key non-methane hydrocarbons (NMHCs) suggest atmospheric concentrations ranging from low pmol/mol to nmol/mol, depending on location and compound. To accurately establish concentration trends and to relate measurement records from many laboratories and researchers, it is essential to have good calibration standards. Several of the world's National Metrology Institutes (NMIs) are developing primary and secondary reference gas standards at the nmol/mol level. While the U.S. NMI, the National Institute of Standards and Technology (NIST), has developed pmol/mol standards for halocarbons and some volatile organics, the feasibility of preparing well-characterized, stable standards for NMHCs at the pmol/mol level is not yet established. NIST recently developed a suite of primary standards by gravimetric dilution that contains 18 NMHCs covering the concentration range of 60 pmol/mol to 230 pmol/mol. Taking into account the small but chemically significant contribution of NMHCs in the high-purity diluent nitrogen used in their preparation, the relative concentrations and short-term stability (2 to 3 months) of these NMHCs in the primary standards have been confirmed by chromatographic analysis. The gravimetric values assigned from the methods used to prepare the materials and the analytical concentrations determined from chromatographic analysis generally agree to within +/-2 pmol/mol. However, anomalous results for several of the compounds reflect the difficulties inherent in avoiding contamination and making accurate measurements at these very low levels.

  7. Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

    Science.gov (United States)

    Wuyts, Véronique; Mattheus, Wesley; Roosens, Nancy H C; Marchal, Kathleen; Bertrand, Sophie; De Keersmaecker, Sigrid C J

    2017-01-01

    A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows one to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension array in which the MOL-PCR products are hybridized to MagPlex-TAG beads.In this chapter, we describe the complete procedure for a MOL-PCR assay for subtyping of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant S. 1,4[5],12:i:- from DNA isolation through heat lysis up to data interpretation through a Gödel Prime Product. The subtyping assay consists of 50 discriminative molecular markers and two internal positive control markers divided over three MOL-PCR assays.

  8. Revision of the gas-phase acidity scale below 300 kcal mol(-1).

    Science.gov (United States)

    Leito, Ivo; Raamat, Elin; Kütt, Agnes; Saame, Jaan; Kipper, Karin; Koppel, Ilmar A; Koppel, Ivar; Zhang, Min; Mishima, Masaaki; Yagupolskii, Lev M; Garlyauskayte, Romute Yu; Filatov, Andrey A

    2009-07-23

    The gas-phase acidity (GA) scale from (CF(3)CO)(2)NH to (C(2)F(5)SO(2))(2)NH--about a 24 kcal mol(-1) range of gas-phase acidities--was reexamined using the Fourier transform ion cyclotron resonance equilibrium measurement approach. Some additions and modifications to the standard methodology of GA measurements were introduced (estimation of partial pressures from mass spectra of the compounds, instead of the pressure gauge readings and use of long reaction times) to achieve higher reliability. Gas-phase acidities of 18 compounds were determined for the first time. The results reveal a contraction of the previously published values in this part of the scale. In particular, the GA values of (CF(3)SO(2))(2)NH and (C(2)F(5)SO(2))(2)NH (important components of lithium ion battery electrolytes and ionic liquids) were revised toward stronger acidities from 291.8 kcal mol(-1) to 286.5 kcal mol(-1) and from 289.4 kcal mol(-1) to 283.7 kcal mol(-1) (i.e., by 5.3 and 5.7 kcal mol(-1)), respectively. Experimental and computational evidence is presented in support of the current results.

  9. CafeMol: A Coarse-Grained Biomolecular Simulator for Simulating Proteins at Work.

    Science.gov (United States)

    Kenzaki, Hiroo; Koga, Nobuyasu; Hori, Naoto; Kanada, Ryo; Li, Wenfei; Okazaki, Kei-Ichi; Yao, Xin-Qiu; Takada, Shoji

    2011-06-14

    For simulating proteins at work in millisecond time scale or longer, we develop a coarse-grained (CG) molecular dynamics (MD) method and software, CafeMol. At the resolution of one-particle-per-residue, CafeMol equips four structure-based protein models: (1) the off-lattice Go model, (2) the atomic interaction based CG model for native state and folding dynamics, (3) the multiple-basin model for conformational change dynamics, and (4) the elastic network model for quasiharmonic fluctuations around the native structure. Ligands can be treated either explicitly or implicitly. For mimicking functional motions of proteins driven by some external force, CafeMol has various and flexible means to "switch" the energy functions that induce active motions of the proteins. CafeMol can do parallel computation with modest sized PC clusters. We describe CafeMol methods and illustrate it with several examples, such as rotary motions of F1-ATPase and drug exports from a transporter. The CafeMol source code is available at www.cafemol.org .

  10. Measures of Biochemical Sociology

    Science.gov (United States)

    Snell, Joel; Marsh, Mitchell

    2008-01-01

    In a previous article, the authors introduced a new sub field in sociology that we labeled "biochemical sociology." We introduced the definition of a sociology that encompasses sociological measures, psychological measures, and biological indicators Snell & Marsh (2003). In this article, we want to demonstrate a research strategy that would assess…

  11. Biochemical Education in Brazil.

    Science.gov (United States)

    Vella, F.

    1988-01-01

    Described are discussions held concerning the problems of biochemical education in Brazil at a meeting of the Sociedade Brazileira de Bioquimica in April 1988. Also discussed are other visits that were made to universities in Brazil. Three major recommendations to improve the state of biochemistry education in Brazil are presented. (CW)

  12. International comparison of a hydrocarbon gas standard at the picomol per mol level.

    Science.gov (United States)

    Rhoderick, George C; Duewer, David L; Apel, Eric; Baldan, Annarita; Hall, Bradley; Harling, Alice; Helmig, Detlev; Heo, Gwi Suk; Hueber, Jacques; Kim, Mi Eon; Kim, Yong Doo; Miller, Ben; Montzka, Steve; Riemer, Daniel

    2014-03-04

    Studies of climate change increasingly recognize the diverse influences of hydrocarbons in the atmosphere, including roles in particulates and ozone formation. Measurements of key nonmethane hydrocarbons (NMHCs) suggest atmospheric mole fractions ranging from low picomoles per mol (ppt) to nanomoles per mol (ppb), depending on location and compound. To accurately establish mole fraction trends and to relate measurement records from many laboratories and researchers, it is essential to have accurate, stable, calibration standards. In February of 2008, the National Institute of Standards and Technology (NIST) developed and reported on picomoles per mol standards containing 18 nonmethane hydrocarbon compounds covering the mole fraction range of 60 picomoles per mol to 230 picomoles per mol. The stability of these gas mixtures was only characterized over a short time period (2 to 3 months). NIST recently prepared a suite of primary standard gas mixtures by gravimetric dilution to ascertain the stability of the 2008 picomoles per mol NMHC standards suite. The data from this recent chromatographic intercomparison of the 2008 to the 2011 suites confirm a much longer stability of almost 5 years for 15 of the 18 hydrocarbons; the double-bonded alkenes of propene, isobutene, and 1-pentene showed instability, in line with previous publications. The agreement between the gravimetric values from preparation and the analytical mole fractions determined from regression illustrate the internal consistency of the suite within ±2 pmol/mol. However, results for several of the compounds reflect stability problems for the three double-bonded hydrocarbons. An international intercomparison on one of the 2008 standards has also been completed. Participants included National Metrology Institutes, United States government laboratories, and academic laboratories. In general, results for this intercomparison agree to within about ±5% with the gravimetric mole fractions of the hydrocarbons.

  13. The Role of Human Chorionic Gonadotropin as Tumor Marker: Biochemical and Clinical Aspects.

    Science.gov (United States)

    Sisinni, Lorenza; Landriscina, Matteo

    2015-01-01

    hCG have been well documented (Tegoni M et al., J Mol Biol 289(5):1375-1385, 1999).

  14. MolTalk – a programming library for protein structures and structure analysis

    Directory of Open Access Journals (Sweden)

    Diemand Alexander V

    2004-04-01

    Full Text Available Abstract Background Two of the mostly unsolved but increasingly urgent problems for modern biologists are a to quickly and easily analyse protein structures and b to comprehensively mine the wealth of information, which is distributed along with the 3D co-ordinates by the Protein Data Bank (PDB. Tools which address this issue need to be highly flexible and powerful but at the same time must be freely available and easy to learn. Results We present MolTalk, an elaborate programming language, which consists of the programming library libmoltalk implemented in Objective-C and the Smalltalk-based interpreter MolTalk. MolTalk combines the advantages of an easy to learn and programmable procedural scripting with the flexibility and power of a full programming language. An overview of currently available applications of MolTalk is given and with PDBChainSaw one such application is described in more detail. PDBChainSaw is a MolTalk-based parser and information extraction utility of PDB files. Weekly updates of the PDB are synchronised with PDBChainSaw and are available for free download from the MolTalk project page http://www.moltalk.org following the link to PDBChainSaw. For each chain in a protein structure, PDBChainSaw extracts the sequence from its co-ordinates and provides additional information from the PDB-file header section, such as scientific organism, compound name, and EC code. Conclusion MolTalk provides a rich set of methods to analyse and even modify experimentally determined or modelled protein structures. These methods vary in complexity and are thus suitable for beginners and advanced programmers alike. We envision MolTalk to be most valuable in the following applications: 1 To analyse protein structures repetitively in large-scale, i.e. to benchmark protein structure prediction methods or to evaluate structural models. The quality of the resulting 3D-models can be assessed by e.g. calculating a Ramachandran-Sasisekharan plot. 2 To

  15. Polygenic expression of teratozoospermia and normal fertility in B10.MOL-TEN1 mouse strain.

    Science.gov (United States)

    Hirawatari, Keitaro; Hanzawa, Naoto; Kuwahara, Maki; Aoyama, Hiroaki; Miura, Ikuo; Wakana, Shigeharu; Gotoh, Hideo

    2015-05-01

    Subfertility and infertility are two major reproductive health problems in human and domestic animals. The contribution of the genotype to these conditions is poorly understood. To examine the genetic basis of male subfertility, we analyzed its relationship to sperm morphology in B10.MOL-TEN1 mice, which shows high-frequencies (about 50%) of morphologically abnormal sperm. Drastic histological changes were also found in the testis of the B10.MOL-TEN1. Segregation analysis showed that the abnormal sperm phenotype in B10.MOL-TEN1 was inherited and was predictably controlled by at least three loci. We also found that male fertility of this strain was normal. These findings indicate a complicated relationship between sperm morphology and male subfertility. © 2015 The Authors. Congenital Anomalies published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Teratology Society.

  16. Medida da elasticidade entrópica de uma molécula de DNA

    OpenAIRE

    Jose Coelho Neto

    2001-01-01

    Neste trabalho combinamos as técnicas de pinçamento óptico e de vídeomicroscopia para estudar o comportamento elástico entrópico de moléculas de DNA de fagos lambda. O método empregado permite a realização de experimentos com uma única molécula por vez. Cada molécula de DNA é presa por uma extremidade a uma microesfera de poliestireno de 3 micra de diâmetro e pela outra a uma lamínula de vidro, formando um análogo microscópico de um sistema massa-mola superamortecido, o qual podemos estirar u...

  17. Type specimens preserved in the Trèmols and Costa herbaria (BC

    Directory of Open Access Journals (Sweden)

    Laura Gavioli

    2016-12-01

    Full Text Available The Trèmols herbarium, preserved at the Botanical Institute of Barcelona, is one of the oldest herbaria in Catalonia, largely made up of European plants collected in 19th century. The digitization of this historical collection, currently in progress, has made possible to locate nomenclatural types of various authors, acquired through exchange. In this article, we indicate two syntypes (for Fumaria caespitosa Loscos and Carex loscosii Lange, and one isolectotype (Polygala vayredae Costa in Trèmols herbarium. Additionally, we designate the lectotype of Polygala vayredae in the Costa herbarium and its isolectotype in the Vayreda herbarium.

  18. Electrical Conductivity of 10 mol% Sc2O3-1 mol% M2O3-ZrO2 Ceramics

    DEFF Research Database (Denmark)

    Omar, Shobit; Bin Najib, Waqas; Chen, Weiwu

    2012-01-01

    The oxide-ion conductivity behavior of 10 mol% Sc2O3-ZrO2 co-doped with 1 mol% trivalent metal oxide has been determined from 350 degrees C to 700 degrees C in air. All the powders were synthesized using conventional solid-oxide route. XRD patterns collected at room temperature show the presence...... of larger ionic radius. The total conductivity of 1M10ScSZ initially increases with increasing size of the co-dopant, reaches a maximum at 0.95 angstrom and thereafter decreases. At 600 degrees C, 1Yb10ScSZ exhibits the highest total conductivity, namely 14 mS/cm. The grain interior and the grain boundary...... follow similar conductivity trends with the maximum at 1In10ScSZ and 1Y10ScSZ, respectively. Around 475 degrees C, 1In10ScSZ but not other compositions exhibits an abrupt drop in the conductivity on cooling, due to the cubic to beta-phase transformation. At 600 degrees C, the activation energy...

  19. MOL-D: A Collisional Database and Web Service within the Virtual ...

    Indian Academy of Sciences (India)

    Page 1. Review. J. Astrophys. Astr., Vol. 36, No. 4, December 2015, pp. 693–703. MOL-D: A Collisional Database and Web Service within the Virtual. Atomic and Molecular Data Center. V. Vujcic1,∗, D. ... coefficients for specific collisional processes and a web service within the. Serbian Virtual Observatory (SerVO) and the ...

  20. Mol-ecular and crystal structure of gossypol tetra-methyl ether with an unknown solvate.

    Science.gov (United States)

    Honkeldieva, Muhabbat; Talipov, Samat; Mardanov, Rustam; Ibragimov, Bakhtiyar

    2015-02-01

    The title compound, C34H38O8 (systematic name: 5,5'-diisopropyl-2,2',3,3'-tetra-meth-oxy-7,7'-dimethyl-2H,2'H-8,8'-bi-[naphtho-[1,8-bc]furan]-4,4'-diol), has been obtained from a gossypol solution in a mixture of dimethyl sulfate and methanol. The mol-ecule is situated on a twofold rotation axis, so the asymmetric unit contains one half-mol-ecule. In the mol-ecule, the hy-droxy groups are involved in intra-molecular O-H⋯O hydrogen bonds, and the two naphthyl fragments are inclined each to other by 83.8 (1)°. In the crystal, weak C-H⋯O and C-H⋯π inter-actions consolidate the packing, which exhibits channels with an approximate diameter of 6 Å extending along the c-axis direction. These channels are filled with highly disordered solvent mol-ecules, so their estimated scattering contribution was subtracted from the observed diffraction data using the SQUEEZE option in PLATON [Spek, A. L. (2015). Acta Cryst. C71, 9-18].

  1. MolPrint3D: Enhanced 3D Printing of Ball-and-Stick Molecular Models

    Science.gov (United States)

    Paukstelis, Paul J.

    2018-01-01

    The increased availability of noncommercial 3D printers has provided instructors and students improved access to printing technology. However, printing complex ball-and-stick molecular structures faces distinct challenges, including the need for support structures that increase with molecular complexity. MolPrint3D is a software add-on for the…

  2. Niveles de biológicos: eficacia e inmunogenicidad del tratamiento para enfermedad inflamatoria intestinal

    OpenAIRE

    Ceballos Santos, Daniel

    2016-01-01

    Programa de doctorado: Avances en Medicina Interna. La fecha de publicación es la fecha de lectura [ES]INTRODUCCIÓN: La monitorización de infliximab (IFX) y adalimumab (ADA) para el control de la actividad de la enfermedad inflamatoria intestinal (EII) y la optimización del tratamiento no está estandarizada, aunque los datos publicados hasta el momento sugieren que tienen una implicación relevante en los resultados de la terapia. Los niveles de biológicos y anticuerpos anti-biológicos pued...

  3. Enseñanza de la Antropología Biológica

    OpenAIRE

    Lomaglio, Delia Beatriz

    2004-01-01

    Durante las Sextas Jornadas Nacionales de Antropología Biológica, llevadas a cabo entre el 8 y el 11 de octubre de 2003, en Catamarca, tuvo lugar la Mesa de Trabajo sobre Enseñanza de la Antropología Biológica, coordinada por la Dra. Delia Lomaglio, de la que participaron docentes e investigadores pertenecientes a universidades de las regiones Norte Grande, Centro y Patagonia de Argentina y colegas extranjeros de España y Uruguay. Los temas tratados fueron: 1. Análisis del estado actual de la...

  4. Digital PSK to BiO-L demodulator for 2 sup nx(bit rate) carrier

    Science.gov (United States)

    Shull, T. A.

    1979-01-01

    A phase shift key (PSK) to BiO-L demodulator which uses standard digital integrated circuits is discussed. The demodulator produces NRZ-L, bit clock, and BiO-L outputs from digital PSK input signals for which the carrier is a 2 to the Nth multiple of the bit rate. Various bit and carrier rates which are accommodated by changing various component values within the demodulator are described. The use of the unit for sinusoidal inputs as well as digital inputs is discussed.

  5. Modelado y simulación de sistemas biológicos

    OpenAIRE

    Cruz Vázquez, Antonio

    2009-01-01

    En la presente memoria se ha recogido de forma escrita el conjunto de fases que se han llevado a cabo en la realización del proyecto consistente en un simulador de sistemas biológicos utilizando modelos orientados al individuo. Concretamente, el sistema biológico representado ha consistido en el movimiento y comportamiento de cohesión que poseen los peces. En la present memòria s'ha recollit de forma escrita el conjunt de fases que s'han portat a terme en la realització del projecte consis...

  6. Desarrollo de normas biológicas que garanticen la seguridad de los pacientes

    OpenAIRE

    Castro Díaz, Ricardo André; Sanchéz Cruz, Stalino Antonino

    2013-01-01

    El presente trabajo intenta desarrollar Normas Biológicas que garanticen la seguridad a los pacientes, que orienten en el uso y manipulación de los equipos e insumos médicos, y que guíen en el manejo de residuos hospitalarios, minimizando los riesgos para el personal de la salud, la comunidad y el medio ambiente, se considerarán Normas Biológicas actuales y extranjeras aplicadas en las áreas de Laboratorio Clínico, Unidad de Cuidados Intensivos (UCI) y Quirófano, en base a ellas se procede a ...

  7. Aislamiento de microorganismos para control biológico de Moniliophthora roreri

    OpenAIRE

    Liliana Yanet Suárez Contreras; Alba Luz Rangel Riaño

    2013-01-01

    Moniliophthora roreri es un hongo que causa la moniliasis, una enfermedad de la mazorca en el cultivo de cacao (Theobroma cacao), con pérdidas hasta del 60% de la cosecha. El control biológico utilizando microorganismos endófitos surge como una alternativa para el manejo de esta enfermedad. En la presente investigación se evaluaron microorganismos con potencial para control biológico de M. roreri en Norte de Santander, Colombia. Para el efecto, se aisló e identificó este fitopatógeno y se uti...

  8. Aproximación biológica al estudio de la emoción

    OpenAIRE

    Palmero, Francisco

    1996-01-01

    El presente trabajo intenta ofrecer una panorámica diacrónica de los estudios sobre Psicología de la Emoción, centrando el tema en la relevancia de las formulaciones biológicas. Se analizan algunas de las aportaciones que, desde un punto de vista biológico, más parecen haber influido en la teoría psicológica de la Emoción. Así, desde los clásicos planteamientos de principio de siglo de James y Cannon, claramente influenciados por las ideas evolucionistas, hasta...

  9. Laboratory hydro-mechanical characterisation of Boom Clay at Essen and Mol

    International Nuclear Information System (INIS)

    Deng, Y. F.; Tang, A. M.; Cui, Y. J.; Nguyen, X. P.; Li, X. L.; Wouters, L.

    2011-01-01

    Boom Clay has been selected as a potential host rock formation for the geological disposal of radioactive waste in Belgium. In the present work, the hydro-mechanical behaviour of Boom Clay samples from the borehole Essen-1 at a depth of 220-260 m and from HADES that is the underground rock laboratory at Mol in Belgium, at 223-m depth was investigated in the laboratory by performing low pressure odometer tests (vertical effective stress ranging from 0.05 to 3.2 MPa), high pressure odometer tests (vertical effective stress ranging from 0.125 to 32 MPa), isotropic consolidation tests (confining effective stress ranging from the in situ stress to 20 MPa) and triaxial shear tests. It has been observed that the mineralogy, geotechnical properties and hydro-mechanical behaviour of Boom Clay from Essen at 227-m, 240-m and 248-m depths are similar to that of Boom Clay from Mol. As in the case of Boom Clay at Mol, the failure envelope of Boom Clay at Essen in the p'- q plane is not linear. The slope of the portion beyond the pre-consolidation stress of Boom Clay from Essen is almost the same as that from Mol, suggesting a similar internal friction angle of about 13 deg. The compression curves (void index I v versus logarithm of vertical stress) beyond the pre-consolidation stress are the same for both samples from Mol and Essen, and situated between the intrinsic compression line (ICL) and the sedimentation compression line (SCL). The yield stress determined from odometer tests seems to be stress-path dependent and lower than the pre-consolidation stress. Thus determining the over-consolidation ratio (OCR) using the yield stress value would lead to an incorrect estimate. From a practical point view, the laboratory test results from Essen and their comparison with those from Mol provide important information regarding the transferability of knowledge on Boom Clay at different sites, taking into account the fact that most investigations have been carried out on Boom Clay at

  10. MolProbity: More and better reference data for improved all-atom structure validation.

    Science.gov (United States)

    Williams, Christopher J; Headd, Jeffrey J; Moriarty, Nigel W; Prisant, Michael G; Videau, Lizbeth L; Deis, Lindsay N; Verma, Vishal; Keedy, Daniel A; Hintze, Bradley J; Chen, Vincent B; Jain, Swati; Lewis, Steven M; Arendall, W Bryan; Snoeyink, Jack; Adams, Paul D; Lovell, Simon C; Richardson, Jane S; Richardson, David C

    2018-01-01

    This paper describes the current update on macromolecular model validation services that are provided at the MolProbity website, emphasizing changes and additions since the previous review in 2010. There have been many infrastructure improvements, including rewrite of previous Java utilities to now use existing or newly written Python utilities in the open-source CCTBX portion of the Phenix software system. This improves long-term maintainability and enhances the thorough integration of MolProbity-style validation within Phenix. There is now a complete MolProbity mirror site at http://molprobity.manchester.ac.uk. GitHub serves our open-source code, reference datasets, and the resulting multi-dimensional distributions that define most validation criteria. Coordinate output after Asn/Gln/His "flip" correction is now more idealized, since the post-refinement step has apparently often been skipped in the past. Two distinct sets of heavy-atom-to-hydrogen distances and accompanying van der Waals radii have been researched and improved in accuracy, one for the electron-cloud-center positions suitable for X-ray crystallography and one for nuclear positions. New validations include messages at input about problem-causing format irregularities, updates of Ramachandran and rotamer criteria from the million quality-filtered residues in a new reference dataset, the CaBLAM Cα-CO virtual-angle analysis of backbone and secondary structure for cryoEM or low-resolution X-ray, and flagging of the very rare cis-nonProline and twisted peptides which have recently been greatly overused. Due to wide application of MolProbity validation and corrections by the research community, in Phenix, and at the worldwide Protein Data Bank, newly deposited structures have continued to improve greatly as measured by MolProbity's unique all-atom clashscore. © 2017 The Protein Society.

  11. Biochemical Hypermedia: Galactose Metabolism.

    Directory of Open Access Journals (Sweden)

    J.K. Sugai

    2013-05-01

    Full Text Available Introduction: Animations of biochemical processes and virtual laboratory environments lead to true molecular simulations. The use of interactive software’s in education can improve cognitive capacity, better learning and, mainly, it makes information acquisition easier. Material and Methods: This work presents the development of a biochemical hypermedia to understanding of the galactose metabolism. It was developed with the help of concept maps, ISIS Draw, ADOBE Photoshop and FLASH MX Program. Results and Discussion: A step by step animation process shows the enzymatic reactions of galactose conversion to glucose-1-phosphate (to glycogen synthesis, glucose-6-phosphate (glycolysis intermediary, UDP-galactose (substrate to mucopolysaccharides synthesis and collagen’s glycosylation. There are navigation guide that allow scrolling the mouse over the names of the components of enzymatic reactions of via the metabolism of galactose. Thus, explanatory text box, chemical structures and animation of the actions of enzymes appear to navigator. Upon completion of the module, the user’s response to the proposed exercise can be checked immediately through text box with interactive content of the answer. Conclusion: This hypermedia was presented for undergraduate students (UFSC who revealed that it was extremely effective in promoting the understanding of the theme.

  12. Las β-(1®3-glucanas: moléculas inmunomoduladoras contaminantes de productos farmacéuticos β-(1®3-glucans as immunomodulating moléculas polluting pharmaceuticals

    Directory of Open Access Journals (Sweden)

    Zenia Pardo Ruiz

    2012-03-01

    Full Text Available Se realizó una búsqueda bibliográfica utilizando la base de datos Pubmed con énfasis en los artículos publicados en la última década. Como descriptores se utilizaron los siguientes: glucans, glucans recognition, glucans biological activitiy, glucans pharmaceuticals. Con la información disponible se realizó un análisis de los principales aspectos relacionados con el tema, que se exponen en el presente trabajo. Las b-(1®3-glucanas son polímeros de glucosa que se encuentran mayoritariamente en la pared celular de hongos, levaduras y plantas. Se consideran patrones moleculares asociados a patógenos y son reconocidas por varios receptores, siendo la dectina-1 el principal receptor de reconocimiento de estas estructuras. Sus propiedades inmunomoduladoras han sido informadas por varios autores. Se ha demostrado que potencian y sinergizan la acción de ligandos de Toll like receptors sobre la liberación de citoquinas proinflamatorias, aunque también han mostrado un perfil antiinflamatorio, cuestión que depende en gran medida de sus características estructurales. Las b-(1®3-glucanas son contaminantes importantes provenientes de los filtros de acetato de celulosa que se utilizan en la clarificación de parenterales hemoderivados, por tanto, es necesario estudiar las consecuencias de la presencia de estas moléculas inmunomoduladoras en inyectables. En esta revisión se resumen aspectos relacionados con el reconocimiento y actividad biológica de las b-(1®3-glucanas y se profundiza en estudios relacionados con su presencia en hemoderivados como principal contaminante. Finalmente se destaca la utilidad de la Prueba de Activación de Monocitos en la detección de las b-(1®3-glucanas en parenterales.A literature review was made in Pubmed database, making emphasis on papers published in the last decade. The subject headings for this search were glucans, glucans recognition, glucans biological activitiy, glucans pharmaceuticals. On the basis

  13. História dos tratamentos biológicos Biologicals treatments's history

    Directory of Open Access Journals (Sweden)

    Sérgio Paulo Rigonatti

    2004-01-01

    Full Text Available Contexto: Trata-se de uma discussão de como surgiram os tratamentos biológicos no decorrer da história da psiquiatria.Context: It's about a discussion on how begun the biological treatment throughout Psychiatry History.

  14. PIRAMID 1: a prime European CEC experiment in BR2 at Mol, Belgium

    International Nuclear Information System (INIS)

    Joly, C.; Simoni, O.

    1987-01-01

    In the event of a core disruptive accident in a Liquid Metal Fast Breeder Reactor (LMFBR), molten core materials interacting with liquid sodium may form debris beds to settle on the retention structures or on the reactor vessel. The decay heat of retained fission products can induce high temperatures or high thermal loads on the retention structures or the reactor vessel with consequent fission product release. To assess the long-term coolability of core debris beds the Commission of the European Communities (CEC) decided to coordinate and fund the European PAHR (Post-Accident Heat Removal) program. The first work carried out for the program resulted in PIRAMID 1 (Pahr IRradiation According to a Mol Integrated Device), a unique irradiation device, designed, constructed and tested at Mol

  15. Contribuições das investigações de Annemarie Mol para a psicologia social

    Directory of Open Access Journals (Sweden)

    Marcia Oliveira Moraes

    2013-06-01

    Full Text Available O artigo apresenta as principais contribuições das investigações realizadas pela médica e filósofa Annemarie Mol para as pesquisas em psicologia social. No campo dos estudos da ciência, tecnologia e sociedade, as investigações realizadas pela filósofa holandesa representam uma guinada para a prática, diferenciando-se do modo como tradicionalmente a teoria ator-rede se situa neste campo. As pesquisas de Mol se caracterizam pela afirmação do caráter performativo das práticas, o que a leva a problematizar os modos de conhecer e intervir das ciências sociais. Por fim são discutidos os alcances das noções de política ontológica e lógica do cuidado para as pesquisas em psicologia social.

  16. Towards J/mol Accuracy for the Cohesive Energy of Solid Argon.

    Science.gov (United States)

    Schwerdtfeger, Peter; Tonner, Ralf; Moyano, Gloria E; Pahl, Elke

    2016-09-26

    The cohesive energies of argon in its cubic and hexagonal closed packed structures are computed with an unprecedented accuracy of about 5 J mol(-1) (corresponding to 0.05 % of the total cohesive energy). The same relative accuracy with respect to experimental data is also found for the face-centered cubic lattice constant deviating by ca. 0.003 Å. This level of accuracy was enabled by using high-level theoretical, wave-function-based methods within a many-body decomposition of the interaction energy. Static contributions of two-, three-, and four-body fragments of the crystal are all individually converged to sub-J mol(-1) accuracy and complemented by harmonic and anharmonic vibrational corrections. Computational chemistry is thus achieving or even surpassing experimental accuracy for the solid-state rare gases. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Microstructure, Crystal structure and ionic conductivity of 3 mol % (Fe, Mn, Co, Zn doped 8YSZ

    Directory of Open Access Journals (Sweden)

    Ahmad Z.A.

    2016-01-01

    Full Text Available Effect of 3 mol% of transition elements doped 8YSZ such as YSZZn, YSZFe, YSZCo and YSZMn on the ionic conductivity. SOFCs mostly operate at higher temperature. By substitute with dopants, it can reduce the operating temperature and costs. In this experiment, 3 mol% dopants mixed with 8YSZ and sintered at 1550 °C, hold for two hours. Crystal structure, microstructure, sintering behaviour and ionic conductivity at 300 oC were investigated. XRD demonstrates three phases (cubic, monoclinic and tetragonal were obtained. It was confirmed that small additions of TMOs (Mn, Fe, Co and Zn promotes densification, grain growth and ionic conductivity compared to pure 8YSZ. YSZZn obtained the highest ionic conductivity, 3.55 × 10−3 mS cm−1 at 300 °C.

  18. The ExoMol pressure broadening diet: H2 and He line-broadening parameters

    Science.gov (United States)

    Barton, Emma J.; Hill, C.; Czurylo, M.; Li, H. Y.; Hyslop, A.; Yurchenko, Sergei N.; Tennyson, Jonathan

    2017-12-01

    In a variety of astronomical objects including gas giant (exo-)planets, brown dwarfs and cool stars, molecular hydrogen and helium are the major line broadeners. However, there is currently no systematic source for these parameters, particularly at the elevated temperatures encountered in many of these objects. The ExoMol project provides comprehensive molecular line lists for exoplanet and other hot atmospheres. The ExoMol database has recently been extended to provide additional data including temperature-dependent, pressure-broadening parameters. Here we assemble H2 and He pressure-broadening datasets for the molecules H2O, NH3, SO2, CH4, PH3, HCN and H2CO using available experimental and theoretical studies.

  19. Evaluation of the anti-inflammatory activities of Quillaja saponaria Mol. saponin extract in mice.

    Science.gov (United States)

    Sarkhel, Sumana

    2016-01-01

    Quillaja saponaria bark contains a high percentage of triterpene saponins and has been used for centuries as antiinflammatory and analgesic agent in Chilean folk medicine. In the Present study the anti-inflammatory activities of the aqueous extract of commercially partially purified saponin from Quillaja saponari a Mol. in in vivo animal models. Aqueous extract of the plant material was prepared by cold maceration. The anti-inflammatory activity of a commercial Quillaja saponaria Mol. (QS) saponin extract was investigated by carragenan induced mice paw edema model for acute inflammation (Winter, 1962) [16]. The anti-inflammatory activity was evaluated by carragenan in paw edema model in swiss albino mice (18-20 g). The anti-inflammatory activity was found to be dose dependent in carragenan induced paw edema. QS was found to significantly ( p  Quillaja saponaria saponins (QS) possess significant anti-inflammatory activity.

  20. La molécule qui sauvera les mangues | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    29 juin 2017 ... La prise de conscience de la valeur de cette molécule date des années 1970, lorsque l'on a compris que l'hexanal pouvait bloquer l'enzyme responsable du vieillissement de la peau des fruits, tout en créant une protection physique contre les agents pathogènes », indique le professeur Jayasankar ...

  1. MolProbity: all-atom structure validation for macromolecular crystallography

    International Nuclear Information System (INIS)

    Chen, Vincent B.; Arendall, W. Bryan III; Headd, Jeffrey J.; Keedy, Daniel A.; Immormino, Robert M.; Kapral, Gary J.; Murray, Laura W.; Richardson, Jane S.; Richardson, David C.

    2010-01-01

    MolProbity structure validation will diagnose most local errors in macromolecular crystal structures and help to guide their correction. MolProbity is a structure-validation web service that provides broad-spectrum solidly based evaluation of model quality at both the global and local levels for both proteins and nucleic acids. It relies heavily on the power and sensitivity provided by optimized hydrogen placement and all-atom contact analysis, complemented by updated versions of covalent-geometry and torsion-angle criteria. Some of the local corrections can be performed automatically in MolProbity and all of the diagnostics are presented in chart and graphical forms that help guide manual rebuilding. X-ray crystallography provides a wealth of biologically important molecular data in the form of atomic three-dimensional structures of proteins, nucleic acids and increasingly large complexes in multiple forms and states. Advances in automation, in everything from crystallization to data collection to phasing to model building to refinement, have made solving a structure using crystallography easier than ever. However, despite these improvements, local errors that can affect biological interpretation are widespread at low resolution and even high-resolution structures nearly all contain at least a few local errors such as Ramachandran outliers, flipped branched protein side chains and incorrect sugar puckers. It is critical both for the crystallographer and for the end user that there are easy and reliable methods to diagnose and correct these sorts of errors in structures. MolProbity is the authors’ contribution to helping solve this problem and this article reviews its general capabilities, reports on recent enhancements and usage, and presents evidence that the resulting improvements are now beneficially affecting the global database

  2. Phenolic profiles of nectar and honey of Quillaja saponaria Mol. (Quillajaceae) as potential chemical markers

    OpenAIRE

    Gloria Montenegro; Javiera Díaz-Forestier; Carolina Fredes; Sharon Rodríguez

    2013-01-01

    Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in t...

  3. Azahar: a PyMOL plugin for construction, visualization and analysis of glycan molecules

    Science.gov (United States)

    Arroyuelo, Agustina; Vila, Jorge A.; Martin, Osvaldo A.

    2016-08-01

    Glycans are key molecules in many physiological and pathological processes. As with other molecules, like proteins, visualization of the 3D structures of glycans adds valuable information for understanding their biological function. Hence, here we introduce Azahar, a computing environment for the creation, visualization and analysis of glycan molecules. Azahar is implemented in Python and works as a plugin for the well known PyMOL package (Schrodinger in The PyMOL molecular graphics system, version 1.3r1, 2010). Besides the already available visualization and analysis options provided by PyMOL, Azahar includes 3 cartoon-like representations and tools for 3D structure caracterization such as a comformational search using a Monte Carlo with minimization routine and also tools to analyse single glycans or trajectories/ensembles including the calculation of radius of gyration, Ramachandran plots and hydrogen bonds. Azahar is freely available to download from http://www.pymolwiki.org/index.php/Azahar and the source code is available at https://github.com/agustinaarroyuelo/Azahar.

  4. Bases biológicas del sueño : Fundamentos biológicos, evolutivos y psicofisiológicos

    OpenAIRE

    Remón Gallo, Diego

    2014-01-01

    El sueño es un proceso común a todo los mamíferos. Estudios realizados con animales y humanos muestran el gran valor evolutivo del sueño en nuestras vidas, en general, y en la influencia para nuestra mente y para la Psicología, en particular. Se hace un recorrido desde las variables biológicas y químicas cerebrales, hasta los ciclos circadianos y los distintos tipos de sueño (SOL y MOR), así como las variaciones psicofisiológicas que se producen como consecuencia de este proceso

  5. molUP: A VMD plugin to handle QM and ONIOM calculations using the gaussian software.

    Science.gov (United States)

    S Fernandes, Henrique; Ramos, Maria J; M F S A Cerqueira, Nuno

    2018-02-21

    The notable advances obtained by computational (bio)chemistry provided its widespread use in many areas of science, in particular, in the study of reaction mechanisms. These studies involve a huge number of complex calculations, which are often carried out using the Gaussian suite of programs. The preparation of input files and the analysis of the output files are not easy tasks and often involve laborious and complex steps. Taking this into account, we developed molUP: a VMD plugin that offers a complete set of tools that enhance the preparation of QM and ONIOM (QM/MM, QM/QM, and QM/QM/MM) calculations. The starting structures for these calculations can be imported from different chemical formats. A set of tools is available to help the user to examine or modify any geometry parameter. This includes the definition of layers in ONIOM calculations, choosing fixed atoms during geometry optimizations, the recalculation or adjustment of the atomic charges, performing SCANs or IRC calculations, etc. molUP also extracts the geometries from the output files as well as the energies of each of them. All of these tasks are performed in an interactive GUI that is extremely helpful for the user. MolUP was developed to be easy to handle by inexperienced users, but simultaneously to be a fast and flexible graphical interface to allow the advanced users to take full advantage of this plugin. The program is available, free of charges, for macOS, Linux, and Windows at the PortoBioComp page https://www.fc.up.pt/PortoBioComp/database/doku.php?id=molup. © 2018 Wiley Periodicals, Inc. © 2018 Wiley Periodicals, Inc.

  6. Moléculas orgánicas obtenidas en simulaciones experimentales del medio interestelar.

    Science.gov (United States)

    Muñoz-Caro, Guillermo Manuel

    Las nubes moleculares son regiones de formación de estrellas, con temperaturas cinéticas entre 10-50 K y densidades de 103-106 átomos cm-3. Su materia está formada por gas y polvo interestelar. Estas partículas de polvo están cubiertas por una fina capa de hielo, de unos 0.01 μm, que contiene H2O y a menudo CO, CO2, CH3OH y NH3. El hielo es presumiblemente irradiado por fotones ultravioleta y rayos cósmicos en las zonas poco profundas de las nubes moleculares y las regiones circunestelares. En un sistema de vacío, P ˜ 10-7 mbar, simulamos la deposición de hielo a partir de 10 K y la irradiación ultravioleta por medio de una lámpara de descarga de hidrógeno activada con microondas. La evolución del hielo se observa por medio de un espectrómetro infrarrojo. De este modo es posible determinar la composición del hielo observado en el medio interestelar y predecir la presencia de moléculas aún no detectadas en el espacio, que han sido producto del procesamiento del hielo en nuestros experimentos. También es posible calentar el sistema hasta temperatura ambiente para sublimar el hielo depositado. Cuando el hielo ha sido previamente irradiado, se observa un residuo compuesto por moléculas orgánicas complejas, algunas prebióticas, como varios ácidos carboxílicos, aminas, amidas, ésteres y en menor proporción moléculas heterocíclicas y aminoácidos. Algunas de estas moléculas podrían detectarse en estado gaseoso por medio de observaciones milimétricas y de radio. También podrían estar presentes en el polvo cometario, cuyo análisis químico está planeado por las misiones Stardust y Rosetta. Mientras tanto, nuestro grupo está llevando a cabo el análisis de partículas de polvo interplanetario (IDPs), algunas de las cuales pueden ser de origen cometario. Al igual que ocurre con los productos obtenidos por irradiación del hielo en nuestros experimentos, algunas IDPs son ricas en material orgánico que contiene oxígeno.

  7. Modelling High Resolution Absorption Spectra with ExoMolLine Lists: NH3and CH4

    DEFF Research Database (Denmark)

    Barton, E. J.; Yurchenko, S. N.; Tennyson, J.

    The conditions, chemical reactions and gas mixing in industrial progresses involving gasification or combustion can be monitored by in situ measurement of gas temperature and gas composition. This can be done spectroscopically, though the result is highly dependent on the quality of reference data...... [1]. For this reason, a smart collaboration has been established between Optical Diagnostics Group at DTU and ExoMol, to combine high resolution spectra measured at elevated temperatures and empirically tuned ab initio methods to produce suitable molecular line lists for modelling molecules...

  8. Fototerapia como Adjuvante da Terapêutica Biológica na Psoríase

    OpenAIRE

    Cabete, J; Serrão, V; Lestre, S; Páris, F; Apetato, M

    2013-01-01

    Introdução: A associação terapêutica é uma estratégia frequentemente usada no tratamento da psoríase. Contudo, a associação de fármacos biológicos com terapêuticas convencionais, e em particular com fototerapia, tem sido pouco explorada. Objectivo: Caracterizar a eficácia e a segurança da associação fototerapia-terapêutica biológica no tratamento da psoríase. Métodos: Foram analisados retrospectivamente os processos clínicos de doentes com o diagnóstico de psoríase vulgar ou artropática...

  9. Flavonoides em Annonaceae: ocorrência e propriedades biológicas

    Directory of Open Access Journals (Sweden)

    Clara dos Reis Nunes

    2012-05-01

    Full Text Available Esta revisão descreve algumas propriedades biológicas e químicas, bem como os usos etnobotânicos e farmacológicos de algumas substâncias isoladas a partir do gênero Annona (família Annonaceae. As espécies dessa família possuem distribuição tropical e subtropical. A Annona muricata L., em especial, possui proeminência na economia de frutos tropicais, além de ser quimicamente caracterizada pela presença de substâncias fenólicas e terpênicas. Logo, este trabalho destaca a importância e ocorrência desses metabólitos, bem como suas atividades biológicas.

  10. Formation of a 25 mol% Fe2O3-Al2O3 solid solution prepared by ball milling

    DEFF Research Database (Denmark)

    Jiang, Jianzhong; Mørup, Steen; Linderoth, Søren

    1996-01-01

    The phase transformation process of a 25 mol% Fe2O3-Al2O3 powder mixture during high-energy ball milling has been studied by x-ray diffraction and Mossbauer spectroscopy. A metastable solid solution of 25 mol % Fe2O3 in Al2O3 with corundum structure has successfully been prepared after a milling...

  11. Entendendo o papel de marcadores biológicos no câncer de pulmão Understanding the role of biological markers in lung cancer

    Directory of Open Access Journals (Sweden)

    VERA LUIZA CAPELOZZI

    2001-11-01

    Full Text Available Marcadores biológicos são componentes celulares, estruturais e bioquímicos, que podem definir alterações celulares e moleculares tanto em células normais quanto aquelas associadas a transformação maligna. Podem ser de dois tipos: 1 marcadores intermediários, que medem alterações celulares e moleculares antes do aparecimento da malignidade; 2 marcadores diagnósticos, presentes em associação com a malignidade. O processo de identificação e validação para uso clínico do marcador tem diversas etapas: identificação inicial feita em linhagens celulares do tumor em questão; teste do marcador em tecido proveniente de biópsias de pacientes com diagnóstico estabelecido do tumor em questão; teste em biópsias de tecidos normais e com processo inflamatório; teste em escarro, sangue ou urina para validação como teste não-invasivo que possa ser usado em população de alto risco. Marcadores biológicos diagnósticos sorológicos e histológicos são componentes celulares, estruturais e bioquímicos, presentes não só em células tumorais como também em células normais, que podem ser medidos quantitativamente por métodos bioquímicos, imunológicos e moleculares nos fluidos ou nos tecidos corporais, respectivamente, associados a neoplasias e possivelmente ao órgão de origem da neoplasia. Marcadores biológicos são estudados em diferentes neoplasias primárias, porém poucos tiveram seu valor clínico definido. O papel dos marcadores biológicos em câncer de pulmão ainda é incerto, pois apenas um pequeno número de marcadores foi avaliado de maneira adequada. O objetivo deste trabalho é entender o papel dos marcadores biológicos sorológicos e diagnósticos no prognóstico e sobrevida de pacientes com câncer de pulmão baseado em uma coletânea de trabalhos realizada pela autora. Apresenta-se também uma perspectiva futura para a detecção precoce do câncer de pulmão baseada no papel dos marcadores biol

  12. Medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad

    Directory of Open Access Journals (Sweden)

    Nancy Burguet Lago

    2013-03-01

    Full Text Available Introducción: el trabajo con microorganismos puede conllevar a riesgos tanto para el personal que trabaja con los mismos como para el medio ambiente. La existencia de laboratorios de seguridad biológica y la implementación de medidas en la manipulación de los agentes biológicos minimizan el riesgo. Objetivo: evaluar las medidas de bioseguridad adoptadas en el manejo con materiales biológicos en Laboratorios Liorad. Métodos: empleo de una lista de chequeo y análisis de los resultados a través de una Matriz DAFO para valorar si el diseño de la instalación cumple con la bioseguridad. Además establecer un sistema documental para la manipulación de microorganismos y la confección de un plan de capacitación para el personal que trabaja en el laboratorio de control microbiológico. Resultados: la lista de chequeo permitió identificar como principal debilidad el no disponer de un doble pasillo para el traslado del material limpio y sucio. Como fortalezas, cumplir con las prácticas y procesamientos adecuados y el contar con equipos de seguridad biológica. El sistema documental incorporó a los procedimientos establecidos para la manipulación, un acápite referido a la «Peligrosidad y Medidas de Seguridad». El programa de capacitación desarrollado permitió proveer conocimientos específicos referidos a esta temática. Conclusión: las medidas adoptadas en el laboratorio permiten plantear que de manera general se cumplen los requisitos establecidos en materia de Bioseguridad para el trabajo con microorganismos.

  13. El biol y las mujeres de Chitacaspi, una experiencia de producción alternativa

    Directory of Open Access Journals (Sweden)

    María Isabel Ríos

    2013-09-01

    Full Text Available El presente artículo describe una práctica de agricultura orgánica en Chitacaspi, una pequeña comuna de Carchi. El análisis de esta experiencia se hace en dos momentos. El primero durante la ejecución del Proyecto Conservación Comunitaria (PCC4 . El segundo se refiere a un grupo de mujeres que elaboran biol y generan ingresos para sus familias.

  14. Tratamiento de aguas industriales mediante reactor biológico de membranas

    OpenAIRE

    Aznar Jiménez, Antonio

    2008-01-01

    El Laboratorio de Ingeniería para el Tratamiento de Aguas de la Universidad Carlos III de Madrid, de investigación y servicios en el tratamiento de aguas residuales, optimiza el diseño y puesta a punto de reactores biológicos de membranas (MBR), indicados para obtener agua depurada de alta calidad y/o aumentar la capacidad de tratamiento.

  15. Manejo biológico de nematodos fitoparásitos con hongos y bacterias

    Directory of Open Access Journals (Sweden)

    Ricardo Piedra Naranjo

    2013-05-01

    Full Text Available La agricultura actual demanda la reducción de plaguicidas químicos y la introducción de sistemas sostenibles con el uso de agentes de manejo biológico.  La alternativa de usar hongos y bacterias para el manejo de nematodos fitoparásitos puede ser una opción biológica muy importante de actualidad. El manejo biológico de nematodos fitoparásitos es posible si se logra fomentar e investigar la utilización de hongos y bacterias que pueden estar en el suelo o, de lo contrario, reproducirse en laboratorios para uso comercial. En revisión de literatura se encontró varios organismos (hongos y bacterias que pueden ser utilizados por agricultores, y en algunos casos, ya están siendo aplicados. Entre los organismos encontrados, tenemos las bacterias Pasteuria penetrans, Pseudomonas (Burlkoldelia cepacia tipo Wisconsin.  Algunos hongos como: Verticillium chlamydosporium. Arthtrobotrys y Monacrosporium, Catenaria spp, Drechmeria coniospora, Pleurotus ostreatus, Pleurotus spp. Fusarium solani, Cylindrocarpon cylindroides y Paecilomyces lilacinus, Verticillium lecanii, Arthrobotrys conoides, A. musiformis, A.  robusta, Monoacrosporium ellipsosporum, Dactylaria thaumasia, Cylindrocarpon sp., Trichoderma harzianum, Dactylella brochophaga, Arthrobotrys oligospora y Arthrobotrys botryospora y Myrothecium verrucaria. Con este tipo de información, planteada desde el punto de vista en investigaciones agropecuarias, se podría tener posibilidades de utilizar nematicidas biológicos en el manejo de nematodos fitoparásitos, desarrollando un manejo integrado de plagas, donde se tendría la posibilidad de no usar de nematicidas químicos en un futuro, o por lo menos reducir sustancialmente su uso en nuestra agricultura y así mejorar la relación con el medio ambiente y el ser humano.

  16. The Influence of biol in the crop yield of Lactuca sativa L. variety Iceberg

    Directory of Open Access Journals (Sweden)

    Pomboza-Tamaquiza Pablo

    2016-11-01

    Full Text Available The objective of the research was to assess the influence of biol enriched with efficient micro-organisms, in the ecological cultivation of Lactuca sativa L (lettuce variety "Iceberg". The field test was carried out on the farm of Querochaca, Faculty of Sciences Agriculture, of the Technical University of Ambato. The studied factors were doses of biol (2%, 4% and 6% and frequencies of application (8 and 15 days, I know used the experimental design of complete blocks at random, with factorial arrangement of 3x2 1 with three repetitions. We evaluated: plant height, diameter of the main stem, days to harvest, weight and diameter of the bud commercial, performance in kg/plot neta and performance in kg/experimental unit, conducted the significance test of Duncan to 5%. The results showed that the dose of 6 per cent applied every 15 days treatment D3F2, contributed to the larger diameter of the bud commercial (25.9 cm; the greater weight of cogollo commercial (1.14 kg and the higher performance (549 kg/experimental unit. This suggests that the implementation of biol can be an important ecological alternative to fertilize organic farming as the lettuce by reducing the use of fertilizers in chemical synthesis and production costs.

  17. Medición de Activos Biológicos en Marcos Conceptuales Comparados

    Directory of Open Access Journals (Sweden)

    Beatriz Brozzú

    2008-12-01

    Full Text Available En este ensayo, no solamente pretendemos seguir probando que las NCP FAPCE (Argentina son la consecuencia de un criterio absolutamente normativista y por lo tanto restrictivo y limitado, mientras que las NIIF y las FASB se fundamentan en un paradigma basado en la “utilidad para el decisor”, o sea en las necesidades del usuario de la información. Lo que también concluiremos es que esta esencial diferencia es la causa por la cual el amplio criterio aplicado por la NIIF 41 –Activos biológicos– y la armoniosa coherencia con su Marco Conceptual, le permiten al Sistema de Información Contable lograr su principal objetivo, cual es mostrar “valor real” para todos los Activos. Por el contrario, y para que las NCP FACPCE argentinas puedan ser realmente un sistema de información útil, y en el caso especifico de la RT22 FAPCE –Activos biológicos–, ésta debe apartarse de su Marco conceptual para poder valuar los Activos Biológicos destinados a ser utilizados en el proceso productivo a un valor corriente (considerado razonablemente representativo de su valor real puesto que de respetar el Marco Conceptual debería valuarlos a valor histórico.

  18. Tecidos biológicos e suas formas de adaptação

    Directory of Open Access Journals (Sweden)

    Hildegard Hedwig Pohl

    2011-07-01

    Full Text Available Os diferentes tecidos biológicos que compõem o sistema locomotor têm propriedades específicas e como decorrência comportamentos diferenciados em resposta ao estresse, não obstante requerem certa quantidade de função e tensão. Considerando que os tecidos biológicos reagem de forma diferente ao estresse a que são submetidos, este artigo objetiva discorrer sobre as propriedades físicas e mecânicas dos tecidos biológicos do sistema locomotor, a fim de possibilitar um maior embasamento nas proposições de intervenção preventivas ou de reabilitação no campo da atividade física. Além disso, consideramos a escassez de material atualizado sobre este assunto, dificultando assim a atualização dos que utilizam deste conhecimento para efetivar sua terapêutica. Para isso, foi abordado cada tecido de forma isolada, para analisarmos suas características individuais e como ocorrem suas adaptações.

  19. Las moléculas de adhesión y la respuesta inmune

    Directory of Open Access Journals (Sweden)

    C. I. Vergara

    1994-09-01

    Full Text Available Las moléculas de adhesión (MA participan en el reconocimiento antigénico y en la migración celular. Determinan el contacto entre varios grupos celulares y la relación entre algunas células y la matriz extracelular, actuando además como señales que contribuyen directamente a la activación celular. Con base en su estructura y características funcionales se pueden agrupar como: superfamilia de las inmunoglobulinas, las integrinas (que a su vez se subdividen en tres grupos: antígenos muy tardíos, integrinas leucocitarias y citoadhesinas y las selectinas (E-selectinas, P-selectinas y L-selectinas. Su mecanismo de acción se ha estudiado en detalle en los procesos de migración leucocitaria que comprende: marginación por medio de selectinas, adhesión al endotelio y quimiotaxis a través de integrinas y, en menor grado, por miembros de la superfamilia de la inmunoglobulinas. La manipulación de los procesos en los cuales intervienen estas moléculas, permitiría que en un futuro sean utilizados como terapia antiinflamatoria en medicina y biología

  20. Evaluation of the anti-inflammatory activities of Quillaja saponaria Mol. saponin extract in mice

    Directory of Open Access Journals (Sweden)

    Sumana Sarkhel

    Full Text Available Objective: Quillaja saponaria bark contains a high percentage of triterpene saponins and has been used for centuries as antiinflammatory and analgesic agent in Chilean folk medicine.In the Present study the anti-inflammatory activities of the aqueous extract of commercially partially purified saponin from Quillaja saponaria Mol. in in vivo animal models. Methods & materials:: Aqueous extract of the plant material was prepared by cold maceration. The anti-inflammatory activity of a commercial Quillaja saponaria Mol. (QS saponin extract was investigated by carragenan induced mice paw edema model for acute inflammation (Winter, 1962 [16]. Results: The anti-inflammatory activity was evaluated by carragenan in paw edema model in swiss albino mice (18–20 g. The anti-inflammatory activity was found to be dose dependent in carragenan induced paw edema. QS was found to significantly (p < 0.05 reduce the carragenan induced mice paw edema (38.59%; 20 mg/kg bw as compared to carragenan control. The percentage inhibition of standard anti-inflammatory drug indomethacin was (55%; 10 mg/kg, bw. Conclusion: The results of the present study demonstrate that the aqueous extract of Quillaja saponaria saponins (QS possess significant anti-inflammatory activity. Keywords: Anti-inflammatory activity, Aqueous extract, Paw edema

  1. Estudio de distribución de tiempos de residencia en un reactor biológico de lecho empacado cerámico

    Directory of Open Access Journals (Sweden)

    Tatiana Rodríguez Chaparro

    2004-01-01

    Full Text Available La distribución de tiempos de residencia de un reactor es una característica del mezclado que ocurre dentro de él [ ] 1 [ ] 2 ; su determinación es básica para el diseño de cualquier tipo de reactor en escala real. El objetivo de esta investigación consistió en determinar la distribución de tiempos de residencia en un reactor biológico de lecho empacado cerámico (anillos a partir de pruebas con trazadores. Los resultados obtenidos utilizando las técnicas de inyección por paso y pulso fueron 34.577 seg., y 17.745 seg., respectivamente, y la dispersión calculada infinita. Lo anterior permite concluir que en reactores de lecho empacado cerámico (anillos las moléculas del trazador se distribuyen uniformemente en todo el sistema. Los ensayos se realizaron en un modelo a escala laboratorio.

  2. Effects of Moringa oleifera leaves as a substitute for alfalfa meal on nutrient digestibility, growth performance, carcass trait, meat quality, antioxidant capacity and biochemical parameters of rabbits.

    Science.gov (United States)

    Sun, B; Zhang, Y; Ding, M; Xi, Q; Liu, G; Li, Y; Liu, D; Chen, X

    2018-02-01

    This contribution reports the effects of Moringa oleifera leaves (MOLs) meal on the growth performances, nutrient digestibility, carcass trait, meat quality, antioxidant capacity and biochemical parameters of growing New Zealand white rabbits. The MOL was substituted for alfalfa meal at levels of 0, 10%, 20% and 30% to obtain respective diets MOL0, MOL10, MOL20 and MOL30. Each treatment was replicated five times with 10 rabbits per replicate. Results showed the average daily weight gain (ADWG) and feed conversion ratio (FCR) of rabbits fed MOL20 diet were significantly better (p oleifera leaves (MOL0, MOL10). The meat drip loss of rabbits fed with diet MOL10 was significantly lower (p oleifera leaves. No significant differences were found in the digestibility of crude fibre (CF), crude fat (EE), ash, crude protein (CP) and nitrogen-free extract (NFE) among the dietary groups. Moringa oleifera leaves also have a significant impact on serum albumin (ALB), low-density lipoprotein cholesterol (LDLC), triiodothyroxine (T 3 ) and tetraiodothyroxine (T 4 ) values and the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and liver. The results indicated that M. oleifera leaves could be developed as a good feed source, and it not only could substitute for alfalfa meal well but also has a significant effect on growth performance, meat quality, antioxidant and biochemical parameters of rabbits. © 2017 Blackwell Verlag GmbH.

  3. Ouroboros - Playing A Biochemical

    Directory of Open Access Journals (Sweden)

    D. T. Rodrigues

    2014-08-01

    Full Text Available Ouroboros: Playing A Biochemical RODRIGUES,D.T.1,2;GAYER, M.C.1,2; ESCOTO, D.F.1; DENARDIN, E.L.G.2, ROEHRS, R.1,2 1Interdisciplinary Research Group on Teaching Practice, Graduate Program in Biochemistry, Unipampa, RS, Brazil 2Laboratory of Physicochemical Studies and Natural Products, Post Graduate Program in Biochemistry, Unipampa, RS, Brazil Introduction: Currently, teachers seek different alternatives to enhance the teaching-learning process. Innovative teaching methodologies are increasingly common tools in educational routine. The use of games, electronic or conventional, is an effective tool to assist in learning and also to raise the social interaction between students. Objective: In this sense our work aims to evaluate the card game and "Ouroboros" board as a teaching and learning tool in biochemistry for a graduating class in Natural Sciences. Materials and methods: The class gathered 22 students of BSc in Natural Sciences. Each letter contained a question across the board that was drawn to a group to answer within the allotted time. The questions related concepts of metabolism, organic and inorganic chemical reactions, bioenergetics, etc.. Before the game application, students underwent a pre-test with four issues involving the content that was being developed. Soon after, the game was applied. Then again questions were asked. Data analysis was performed from the ratio of the number of correct pre-test and post-test answers. Results and discussion: In the pre-test 18.1% of the students knew all issues, 18.1% got 3 correct answers, 40.9% answered only 2 questions correctly and 22.7% did not hit any. In post-test 45.4% answered all the questions right, 31.8% got 3 questions and 22.7% got 2 correct answers. The results show a significant improvement of the students about the field of content taught through the game. Conclusion: Generally, traditional approaches of chemistry and biochemistry are abstract and complex. Thus, through games

  4. Optimization of photosynthesis, growth, and biochemical composition of the microalga Rhodomonas salina

    DEFF Research Database (Denmark)

    Thuy, Minh Vu Thi; Douëtte, Claire; Rayner, Thomas Allan

    2016-01-01

    The cryptophyte Rhodomonas salina is widely used as feed for copepod cultures. However, culturing conditions to obtain high-quality algae have not yet been efficiently optimized. Therefore, we aimed to develop a cultivation protocol for R. salina to optimize its nutritional value and provide tech...... of irradiance 60–100 μmol photons m−2 s−1 and nutrient excess to obtain algae with high production and a balanced biochemical composition as feed for copepods....

  5. RichMol: A general variational approach for rovibrational molecular dynamics in external electric fields

    Science.gov (United States)

    Owens, Alec; Yachmenev, Andrey

    2018-03-01

    In this paper, a general variational approach for computing the rovibrational dynamics of polyatomic molecules in the presence of external electric fields is presented. Highly accurate, full-dimensional variational calculations provide a basis of field-free rovibrational states for evaluating the rovibrational matrix elements of high-rank Cartesian tensor operators and for solving the time-dependent Schrödinger equation. The effect of the external electric field is treated as a multipole moment expansion truncated at the second hyperpolarizability interaction term. Our fully numerical and computationally efficient method has been implemented in a new program, RichMol, which can simulate the effects of multiple external fields of arbitrary strength, polarization, pulse shape, and duration. Illustrative calculations of two-color orientation and rotational excitation with an optical centrifuge of NH3 are discussed.

  6. PyMine: a PyMOL plugin to integrate and visualize data for drug discovery.

    Science.gov (United States)

    Chaudhari, Rajan; Li, Zhijun

    2015-10-01

    Tremendous amount of chemical and biological data are being generated by various high-throughput biotechnologies that could facilitate modern drug discovery. However, lack of integration makes it very challenging for individual scientists to access and understand all the data related to a specific protein of interest. To overcome this challenge, we developed PyMine, a PyMOL plugin that retrieves chemical, structural, pathway and other related biological data of a receptor and small molecules from a variety of high-quality databases and presents them in a graphic and uniformed way. Developed as an interactive and user-friendly tool, PyMine can be used as a central data-hub for users to access and visualize multiple types of data and to generate new ideas intuitively for structure-based molecule design.

  7. Beryllium(II) hydrolysis in 3.0 mol dm-3 perchlorate

    International Nuclear Information System (INIS)

    Bruno, Jordi

    1987-01-01

    The complex-formation equilibria in the system Be 11 -H 2 O have been studied at 25 0 C by means of e.m.f. methods, using a coulometric titration technique, in 3.0 mol dm -3 (Na)ClO 4 solutions. The ranges of total beryllium(II) concentration and acidity were 80 > = B >= 1 mmol dm -3 and 2 21 0 = -3.47 +- 0.05, log β 33 0 = -8.86 +- 0.05, log β 56 0 = -19.5 +- 0.1, and log β 68 0 = -26.3 +- 0.1. Tentative structures for the polynuclear hydroxo complexes are proposed and discussed. The solubility product of α- Be(OH) 2 (s) has been re-evaluated by using literature data and the hydrolysis constants of this study, at l = 0, log Ksub(so) = -6.87 +- 0.05. (author)

  8. Elaboration and evaluation of maqui juice (Aristotelia chilensis (Mol. Stuntz by steam drag

    Directory of Open Access Journals (Sweden)

    Ximena Araneda

    2014-09-01

    Full Text Available The aim of this study was develop and evaluate maqui juice (Aristotelia chilensis (Mol. Stuntz, to be potentially considered as a functional beverage of natural origin, without chemical additives and minimally processed, using the technique of steam drag of type artisanal. Fruit harvested manually was used in the Region of The Araucanía (Chile. Two juice concentrates with sugar and without sugar were produced. Analyzes such as were conducted: content of soluble solids, pH, acidity, moisture content, dry matter (DM, total ash, total sugars (AT, crude protein (PC, total polyphenols (PFT and total carbohydrates (CHT, the polyphenol content highlighting for unsweetened juice with 993.2 mg 100 mL-1 EAG and juice with sugar 829.208 mg 100 mL-1 EAG. Therefore, the technique allows to extract juice with minimal processing machin, presenting this high concentration of polyphenols.

  9. The atomistic structure of yttria stabilised zirconia at 6.7 mol%: an ab initio study.

    Science.gov (United States)

    Parkes, Michael A; Tompsett, David A; d'Avezac, Mayeul; Offer, Gregory J; Brandon, Nigel P; Harrison, Nicholas M

    2016-11-16

    Yttria stabilized zirconia (YSZ) is an important oxide ion conductor used in solid oxide fuel cells, oxygen sensing devices, and for oxygen separation. Doping pure zirconia (ZrO 2 ) with yttria (Y 2 O 3 ) stabilizes the cubic structure against phonon induced distortions and this facilitates high oxide ion conductivity. The local atomic structure of the dopant is, however, not fully understood. X-ray and neutron diffraction experiments have established that, for dopant concentrations below 40 mol% Y 2 O 3 , no long range order is established. A variety of local structures have been suggested on the basis of theoretical and computational models of dopant energetics. These studies have been restricted by the difficulty of establishing force field models with predictive accuracy or exploring the large space of dopant configurations with first principles theory. In the current study a comprehensive search for all symmetry independent configurations (2857 candidates) is performed for 6.7 mol% YSZ modelled in a 2 × 2 × 2 periodic supercell using gradient corrected density functional theory. The lowest energy dopant structures are found to have oxygen vacancy pairs preferentially aligned along the 〈210〉 crystallographic direction in contrast to previous results which have suggested that orientation along the 〈111〉 orientation is favourable. Analysis of the defect structures suggests that the Y 3+ -O vac interatomic separation is an important parameter for determining the relative configurational energies. Current force field models are found to be poor predictors of the lowest energy structures. It is suggested that the energies from a simple point charge model evaluated at unrelaxed geometries is actually a better descriptor of the energy ordering of dopant structures. Using these observations a pragmatic procedure for identifying low energy structures in more complicated material models is suggested. Calculation of the oxygen vacancy migration activation

  10. ExoMol molecular line lists - XXVI: spectra of SH and NS

    Science.gov (United States)

    Yurchenko, Sergei N.; Bond, Wesley; Gorman, Maire N.; Lodi, Lorenzo; McKemmish, Laura K.; Nunn, William; Shah, Rohan; Tennyson, Jonathan

    2018-04-01

    Line lists for the sulphur-containing molecules SH (the mercapto radical) and NS are computed as part of the ExoMol project. These line lists consider transitions within the X 2Π ground state for 32SH, 33SH, 34SH and 32SD, and 14N32S, 14N33S, 14N34S, 14N36S and 15N32S. Ab initio potential energy (PEC) and spin-orbit coupling (SOC) curves are computed and then improved by fitting to experimentally observed transitions. Fully ab initio dipole moment curves (DMCs) computed at high level of theory are used to produce the final line lists. For SH, our fit gives a root-mean-square (rms) error of 0.03 cm-1 between the observed (vmax = 4, Jmax = 34.5) and calculated transitions wavenumbers; this is extrapolated such that all X 2Π rotational-vibrational-electronic (rovibronic) bound states are considered. For 32SH the resulting line list contains about 81 000 transitions and 2 300 rovibronic states, considering levels up to vmax = 14 and Jmax = 60.5. For NS the refinement used a combination of experimentally determined frequencies and energy levels and led to an rms fitting error of 0.002 cm-1. Each NS calculated line list includes around 2.8 million transitions and 31 000 rovibronic states with a vibrational range up to v = 53 and rotational range to J = 235.5, which covers up to 23 000 cm-1. Both line lists should be complete for temperatures up to 5000 K. Example spectra simulated using this line list are shown and comparisons made to the existing data in the CDMS database. The line lists are available from the CDS (http://cdsarc.u-strasbg.fr) and ExoMol (www.exomol.com) data bases.

  11. Propriedades eletrônicas e espectroscópicas de aglomerados de moléculas interestelares

    OpenAIRE

    Arnaldo Machado da Silva

    2013-01-01

    O meio interestelar é um gigantesco reservatório de átomos e moléculas de vários tamanhos e formas. Em geral, eles estão concentrados em regiões muito grandes conhecidas como nuvens interestelares. Existe um grande interesse em detectar e compreender melhor as estruturas eletrônicas, as propriedades e as reações dessas moléculas interestelares. Com o desenvolvimento de equipamentos de radioastronomia cada vez mais sofisticado, foram detectadas mais de 150 moléculas no espaço interestelar até ...

  12. Protective effect of Lagenaria siceraria (Mol) against membrane-bound enzyme alterations in isoproterenol-induced cardiac damage in rats.

    Science.gov (United States)

    Vijayakumar, M; Selvi, V; Krishnakumari, S

    2012-01-01

    This study was aimed at evaluating the preventive role of the ethanolic extract of Lagenaria siceraria (Mol) fruit on membrane-bound enzymes, such as sodium potassium-dependent adenosine triphosphatase (Na(+)/K(+) ATPase), calcium-dependent adenosine triphosphatase (Ca(2+) ATPase) and magnesium-dependent adenosine triphosphatase (Mg(2+) ATPase) on isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Male albino Wistar rats were pretreated with the ethanolic extract of L. siceraria (Mol) fruit (125, 250 and 500 mg kg(-1) body weight) for a period of 30 days. After the treatment period, ISO (85mg kg(-1) body weight) was subcutaneously injected into rats at 24-h intervals for 2 days. ISO-induced rats showed a significant (p Mol) fruit for a period of 30 days exhibited a significant (p Mol) fruit has membrane-stabilising role in ISO-induced MI in rats.

  13. Biolingüística y capacidad humana Biolinguistics and Human Capacity

    Directory of Open Access Journals (Sweden)

    Chomsky Noam Abraham

    2006-06-01

    Full Text Available Este artículo presenta de manera sucinta el enfoque llamado 'biolingüístico' que empezó a desarrollarse en los primeros años de la posguerra, teniendo en cuenta los avances de la biología y las matemáticas. Se pasa revista a algunos postulados de la neurociencia (i.e. ciencia cognitiva en lo concerniente a la 'facultad del lenguaje', así como a los dos últimos modelos de la GG: el modelo de 'Principios y Parámetros' (P&P y el modelo 'Minimalista'. Estos últimos buscan responder las cuestiones fundamentales de la biología del lenguaje, su naturaleza, uso y evolución. Desde este punto de vista, se trata de caracterizar el equipamiento biológico gracias al cual los seres humanos adquieren una gramática. Así las cosas, se considera el equipamiento biológico como una función que asocia experiencia y gramática.This paper depicts a brief account of the so-called ´biolinguistic´ approach which dates back to the first postwar years, by taking into account progress in biology and mathematics. Some postulates of neuroscience (i.e. cognitive science regarding the ´faculty of language´ as well as the last two models of GG: the ´Principles and Parameters´ (P&P and the ´Minimalist´ models are reviewed. The latter aim to provide answers to key questions on the biology of language, its nature, its use, and its evolution. From this standpoint it has been attempted to characterize the biological endowment which allows human beings to acquire a grammar. Thus the biological endowment is seen as a function relating experience to grammar.

  14. Oestrosis. Aspectos Biológicos de la miasis cavitaria ovina

    OpenAIRE

    Héctor M. Puebla Domínguez; Nelson Zaldivar Quintero; Rafael Soñora Bonilla

    2005-01-01

    En el trabajo se hace referencia a los aspectos biológicos de la miasis cavitaria ovina (oestrus ovis). La entidad parasitaria se enriquece con las últimas informaciones disponibles en internet procedentes de numerosas investigaciones realizadas por otros colegas en el mundo. Nuestro país fue afectado por un brote de la enfermedad, la cual fue reportada en la provincia de Guantánamo en la zona de yacabo abajo del municipio de Imías. Se realizaron investigaciones clínico-anatomopatológicas en...

  15. Impacto nutricional de la terapia biológica en pacientes oncológicos

    OpenAIRE

    Salazar González, Marta

    2015-01-01

    La malnutrición es un factor determinante en pacientes oncológicos, siendo considerado como factor pronóstico de la enfermedad. La desnutrición no es causada solo por el tumor, sino que también por los tratamientos recibidos para su cura. Uno de esos tratamientos es la terapia biológica, también denominada inmunoterapia, la cual no ha sido muy estudiada para determinar el impacto nutricional que conlleva a estos tipos de pacientes. Debido a la poca información disponible sobre el impa...

  16. Terapias biológicas contra el cáncer

    OpenAIRE

    Barba Villanueva, Sara; Prieto Montón, Juan

    2016-01-01

    El cáncer es una de las enfermedades más importantes a nivel mundial, tanto en incidencia como en mortalidad. La terapia antineoplásica por excelencia es la quimioterapia, basada en fármacos de acción inespecífica que los hacen presentar una elevada toxicidad y efectos secundarios. Con el objetivo de solucionar estos problemas, se están desarrollando cada vez más una serie de tratamientos dirigidos diseñados para bloquear de forma específica el proceso tumoral. Así, las terapias biológicas...

  17. Aspectos biológicos de Microcharops anticarsiae (Hymenoptera: Ichneumonidae) parasitando Anticarsia gemmatalis (Lepidoptera: Erebidae)

    OpenAIRE

    Aguirre-Gil, Oniel Jeremías [UNESP

    2016-01-01

    Microcharops anticarsiae é um eficiente parasitoide da lagarta-dasoja Anticarsia gemmatalis que, também, parasita lagartas de outras espécies como Chrysodeixis includens e Spodoptera eridania. O objetivo do trabalho foi (1) determinar o ínstar larval de A. gemmatalis preferencialmente parasitado por M. anticarsiae a partir de lagartas coletadas em campo, (2) determinar o efeito de criações sucessivas de M. anticarsiae nos parâmetros biológicos do parasitoide e (3) determinar o efeito da soja ...

  18. Comportamento do consumidor : determinantes no consumo de alimentos biológicos

    OpenAIRE

    Fonseca, Nádia Mariana Rodrigues

    2016-01-01

    Dissertação de mestrado em Marketing, apresentada à Faculdade de Economia da Universidade de Coimbra, sob a orientação de Arnaldo Coelho. O consumo de alimentos biológicos tem aumentado ao longo dos anos, de modo que a compreensão do comportamento e do perfil do consumidor de alimentos orgânicos é importante, especialmente para o setor da indústria alimentar. O objetivo deste estudo é identificar e caraterizar alguns aspetos do comportamento e do perfil do consumidor de aliment...

  19. La eficacia biológica de la explotación pascicola

    OpenAIRE

    Spedding, C. R.W.; Hoxey, Angela M.

    2011-01-01

    La eficacia biológica de la explotación de los pastos puede ser expresadas en cantidad de producto (s) obtenido por unidad de cualquiera de los recursos utilizados. Estas relaciones se pueden utilizar para elegir las mejoras técnicas que se han de llevar a cabo dentro de un contexto económico. La contribución más eficiente de los pastos para la producción de alimentos para el hombre es probable que esté ligada a los sistemas que suponen el consumo directo de la hierba por el ganado en lugar d...

  20. Bioseguridad y armas biológicas: La amenaza de Andrómeda (1971)

    OpenAIRE

    Sánchez Angulo, Manuel

    2011-01-01

    La amenaza de Andrómeda es la adaptación cinematográfica de la obra homónima escrita por Michael Crichton. Puede considerarse como la película más “microbiológica” del Séptimo Arte. Dirigida por Robert Wise, este tecno-thriller describe los esfuerzos de un equipo de científicos para caracterizar y contener a un microorganismo patógeno extraterrestre. A pesar de haber sido realizada hace 40 años, se tratan temas de plena actualidad como es el de las armas biológicas, las medidas de aislamiento...

  1. Heterocronía, generadora de cambios biológicos

    OpenAIRE

    Picasso, Mariana

    2007-01-01

    El reloj biológico se ha acelerado o retrasado reiteradas veces a lo largo de la historia de los seres vivos. De este modo, se han modificado tanto los tiempos del desarrollo general del cuerpo, como de una estructura en particular, e incluso el tiempo en el que se produce la maduración sexual del organismo. Estos cambios temporales se denominan eventos de heterocronía (del griego, heteros: distinto, chronos: tiempo) y son una piedra angular del proceso evolutivo. Las heterocronías son respon...

  2. Observación de muestras biológicas al microscopio óptico

    OpenAIRE

    Alvarez Hornos, Francisco Javier; Izquierdo Sanchis, Marta

    2013-01-01

    La observación microscópica de muestras de aguas de los reactores biológicos de una Estación Depuradora de Aguas Residuales proporciona información cualitativa sobre la calidad de la población microbiana y del estado de los flóculos. En este vídeo, se muestra la utilización de un microscopio óptico para la observación de muestras de agua de un reactor de fangos activados. Data: 2013 ;

  3. La vida y la sensibilidad de la materia biológica en la filosofía de Xavier Zubiri

    Directory of Open Access Journals (Sweden)

    Marcos Louis Catalán, Jr.

    2015-02-01

    Full Text Available Este ensayo expone las ideas zubirianas acerca de la materia biológica, que la materia —la esencia de las sustantividades materiales— da de sí por su potencialidad de sistematización. El animal es una estructura biológica que es dinámicamente estable y reversible. Su vida y su sensibilidad son propiedades sistémicas. Como propiedades, son combinaciones funcionales que emergen de las actividades respectivas de las moléculas orgánicas componentes del animal. La vida no es una fuerza, sino un principio dominante que orienta las acciones moleculares. En cuanto a la sensibilidad animal, Zubiri la analiza en tres niveles: el proceso sentiente, la habitud y las estructuras constitutivas. La suscitación, la modificación tónica y la respuesta son los tres momentos constitutivos del indivisible proceso sentiente. Habitud, o la manera de enfrentarse con las cosas, subyace el proceso sentiente y es el fundamento de toda suscitación y de toda respuesta. La habitud propia del animal es sensibilidad, y la formalidad correspondiente es la formalidad del mero estímulo. Bajo esta formalidad, el animal aprehende los estímulos sólo como un signo de respuesta y nada más. El proceso sentiente y la habitud, a su vez, se hacen posible por las estructuras constitutivas del animal, particularmente, por su sistema nervioso. La evolución morfológica de este sistema se puede entender desde la formalización: Cuanto más formalizado es el sistema nervioso, tanto más variados son las percepciones (suscitación, los afectos (modificación tónica y las posibles respuestas del animal. Pero cualquiera que sea el grado de formalización de su sistema nervioso, el animal nunca puede dejar de aprehender algo bajo la formalidad del mero estímulo.

  4. Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants.

    Science.gov (United States)

    Caro, L A; Polci, P A; Lindström, L I; Echenique, C V; Hernández, L F

    2002-04-01

    Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P. chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1-2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated with 0.05 mg L-1 BA and 3 mg L-1 of either IAA, IBA or NAA. Sucrose (3% w/v) was used as carbon source. The percentage of sprouted cuttings and whole plant regeneration as well as its shoot and root length were recorded. Number, length and dry weight of shoots and roots were also measured. Rooting was successful with cuttings taken from young or adult plants, but explants from young plants showed a better response. Culturing in BTMm resulted in significantly greater shoot and root biomass than culturing in MS. Moreover, this response was higher in young explants when IBA was used as growth regulator. This paper reports a simple and effective method to micropropagate P. chilensis from young and adult plants.

  5. [Use of mesquite cotyledon (Prosopis chilensis (Mol) Shuntz) in the manufacturing of cereal bars].

    Science.gov (United States)

    Estévez, A M; Escobar, B; Ugarte, V

    2000-06-01

    Cereal bars with peanut and walnut has shown to be snack foods of good organoleptic characteristics and high caloric value, due to their content of protein, lipids and carbohydrates. Cotyledons of mezquite seeds have a high protein content which biological quality improves with thermal processing like toasting, microwave or moist heat under pressure. The purposes of this research were to study the use of mezquite cotyledon (Prosopis chilensis (Mol) Stuntz) in cereal bars with two different levels of peanut or walnut; and to determine the effect of two thermal treatment applied on the cotyledon upon the bar characteristics. Twelve different kind of bars were developed through the combination of two levels of peanut or walnut (15% and 18%); the use of mezquite cotyledon (0% and 6%); and the application of two thermal processing to the cotyledon (microwave and toasting). Cereal bars were analysed for chemical, physical and sensory characteristics: moisture, water activity, proximate chemical composition, sensory quality and acceptability. Moisture content of bars with peanut ranged between 10.4% and 10.9%; and for those with walnut, between 10.5% and 12.3%. Protein content was higher in the bars with mezquite cotiledon, being higher those with peanut. Thermal processing did not have any effect on the chemical composition. Bars with mezquite cotyledon treated by microwave showed a higher acceptability.

  6. molSimplify: A toolkit for automating discovery in inorganic chemistry.

    Science.gov (United States)

    Ioannidis, Efthymios I; Gani, Terry Z H; Kulik, Heather J

    2016-08-15

    We present an automated, open source toolkit for the first-principles screening and discovery of new inorganic molecules and intermolecular complexes. Challenges remain in the automatic generation of candidate inorganic molecule structures due to the high variability in coordination and bonding, which we overcome through a divide-and-conquer tactic that flexibly combines force-field preoptimization of organic fragments with alignment to first-principles-trained metal-ligand distances. Exploration of chemical space is enabled through random generation of ligands and intermolecular complexes from large chemical databases. We validate the generated structures with the root mean squared (RMS) gradients evaluated from density functional theory (DFT), which are around 0.02 Ha/au across a large 150 molecule test set. Comparison of molSimplify results to full optimization with the universal force field reveals that RMS DFT gradients are improved by 40%. Seamless generation of input files, preparation and execution of electronic structure calculations, and post-processing for each generated structure aids interpretation of underlying chemical and energetic trends. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  7. In vitro propagation of Caesalpinia spinosa (Mol. O. Kuntz from axillary buds of selected trees

    Directory of Open Access Journals (Sweden)

    Jenny E Núñez Núñez

    2017-07-01

    Full Text Available Guarango or tara [Caesalpinia spinosa (Mol. O. Kuntz] is a tree native to the Andes, with great economic importance and for reforestation programs. The aim of this work was to in vitro propagate this specie from axillary buds of selected trees. During in vitro establishment, the effect of sodium hypochlorite (3.0% with different times of disinfection (5.0, 10, 15 min, as well as the effect of 6-BAP on the in vitro response of buds were studied. For multiplication, different combination of 6-BAP with 0.1 mg l-1 ANA were tested. A free-growth regulator culture medium was used for rooting. The best results for in vitro establishment were achieved with a disinfection treatment with sodium hypochlorite 3.0% for 10 minutes and cultivation in a culture medium with 0.25 mg l-1 6-BAP, which 90% of buds in vitro established, with a length of 6.71 cm. The highest multiplication rate of shoot (2.88 per explant was obtained with 1.0 mg l-1 6-BAP and 0.1 mg l-1 ANA, after 60 days of culture. The 55% of these shoots developed roots in a half-strength basal salts MS culture medium free of regulators of growth.   Keywords: biodiversity, conservation, forest plant, guarango, tissue culture

  8. The bent crystal diffraction spectrometer at the BR2 reactor in Mol

    Science.gov (United States)

    Kaerts, E.; Jacobs, L.; Vandenput, G.; Van Assche, P. H. M.

    1988-05-01

    The DuMond-type bent crystal diffraction spectrometer installed at the BR2 reactor in Mol is presented. The spectrometer is mainly designed to study nuclear γ-transitions following thermal neutron capture. It covers the energy interval 25 ≦ Eγ ≦ 1500 keV. Instead of the traditionally used quartz crystals, a highly perfect silicium crystal is chosen as analysing crystal. Diffraction occurs from the (220) plane. The "quasi-mosaic" width, introduced by bending the crystal, is as small as 0.2″. The integrated reflecting power R of the bent crystal stays constant up to 1.5 MeV in first, 680 keV in second and 300 keV in third diffraction order. For higher photon energies, only an E-1 energy dependence is observed in second and third diffraction order. Consequently, besides improving the energy resolution, the use of these silicium crystals substantially increases the spectrometer efficiency and extends the high energy limit of bent crystal diffraction spectrometers. The diffraction angles are measured with a symmetrical interferometer system which covers an angular range of -6° to +6° with a precision of about 0.01″. Minimum diffraction line widths of 0.9″ have been measured, corresponding to an energy resolution ΔE = 1.35 × 10 -6E2n-1 keV -1. The dominant contribution to the observed line widths arises from the finite extent of the source.

  9. Por uma Psicologia Social não perspectivista: contribuições de Annemarie Mol

    Directory of Open Access Journals (Sweden)

    Mariana Prioli Cordeiro

    2013-01-01

    Full Text Available En este texto, buscamos discutir las contribuciones de la obra de Annemarie Mol a la Psicología Social. Para hacerlo, presentamos algunas de sus reflexiones centrales, tales como el abordaje no perspectivista de la realidad y las nociones de multiplicidad y política ontológica. Al proponer esta discusión, queremos llamar la atención para la posibilidad de ordenar y coordinar la realidad de diferentes maneras, así como también reconocer que un fenómeno está formado por múltiples y diversos actores. Buscamos, por lo tanto, defender una Psicología Social que se acerca de la Teoría del Actor-Red, al buscar conexiones complejas, que articulen humanos a no humanos y que performen diversas realidades. Una Psicología Social que huya del realismo que caracteriza gran parte de las investigaciones científicas y, por lo tanto, que no presuponga una realidad anterior e independiente de nuestras acciones, sino una realidad que se vuelva real por intermedio de nuestras prácticas.

  10. Phenolic profiles of nectar and honey of Quillaja saponaria Mol. (Quillajaceae as potential chemical markers

    Directory of Open Access Journals (Sweden)

    Gloria Montenegro

    2013-01-01

    Full Text Available Quillaja saponaria Mol. (Quillajaceae is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection showed a similar profile of phenolic compounds, in which gallic acid, myricetin, rutin, quercetin and naringenin were identified. The phenolic compounds detected could be used as a reference for future studies for determining potential chemical markers of this honey, complementing the present identification of honeys by determining their botanical origin. The identification of bioindicators of the floral origins for honey of this species could provide added value to honey commercialization by certifying the botanical origin of their chemical features and biological attributes.

  11. Topical anti-inflammatory activity of quillaic acid from Quillaja saponaria Mol. and some derivatives.

    Science.gov (United States)

    Rodríguez-Díaz, Maité; Delporte, Carla; Cartagena, Carlos; Cassels, Bruce K; González, Patricia; Silva, Ximena; León, Fredy; Wessjohann, Ludger A

    2011-05-01

    Quillaic acid is the major aglycone of the widely studied saponins of the Chilean indigenous tree Quillaja saponaria Mol. The industrial availability of quillaja saponins and the extensive functionalization of this triterpenoid provide unique opportunities for structural modification and pose a challenge from the standpoint of selectivity in regard to one or the other secondary alcohol group, the aldehyde, and the carboxylic acid functions. The anti-inflammatory activity of this sapogenin has not been studied previously and it has never been used to obtain potential anti-inflammatory derivatives. A series of quillaic acid derivatives were prepared and subjected to topical assays for the inhibition of inflammation induced by arachidonic acid or phorbol ester. Quillaic acid exhibited strong topical anti-inflammatory activity in both models. Most of its derivatives were less potent, but the hydrazone 8 showed similar potency to quillaic acid in the TPA assay. The structural modifications performed and the biological results suggest that the aldehyde and carboxyl groups are relevant to the anti-inflammatory activity in these models. © 2011 The Authors. JPP © 2011 Royal Pharmaceutical Society.

  12. Phenolic profiles of nectar and honey of Quillaja saponaria Mol. (Quillajaceae) as potential chemical markers.

    Science.gov (United States)

    Montenegro, Gloria; Díaz-Forestier, Javiera; Fredes, Carolina; Rodríguez, Sharon

    2013-01-01

    Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection) showed a similar profile of phenolic compounds, in which gallic acid, myricetin, rutin, quercetin and naringenin were identified. The phenolic compounds detected could be used as a reference for future studies for determining potential chemical markers of this honey, complementing the present identification of honeys by determining their botanical origin. The identification of bioindicators of the floral origins for honey of this species could provide added value to honey commercialization by certifying the botanical origin of their chemical features and biological attributes.

  13. Guerra biológica, bioterrorismo e saúde pública

    Directory of Open Access Journals (Sweden)

    Luiz Jacintho da Silva

    Full Text Available O uso de agentes biológicos como arma não é novidade para a humanidade. Durante séculos, até a atualidade, a guerra biológica tem sido objeto de muita pesquisa e especulação, mas de pouca ação. O medo de efeitos contrários e dúvidas sobre sua eficiência como armas devem ter limitado seu uso. Recentemente, se verificou uma nova forma de terrorismo, empregando agentes infecciosos, devagar e sem muito alarde, até as ocorrências recentes com o Bacillus anthracis nos Estados Unidos. A varíola é possivelmente o mais devastador desses agentes. Menos de 25 anos passados desde sua erradicação, a saúde pública tem que lidar com a possibilidade de sua re-introdução. O cenário da re-introdução da varíola no Brasil é discutido.

  14. Guerra biológica, bioterrorismo e saúde pública

    Directory of Open Access Journals (Sweden)

    Silva Luiz Jacintho da

    2001-01-01

    Full Text Available O uso de agentes biológicos como arma não é novidade para a humanidade. Durante séculos, até a atualidade, a guerra biológica tem sido objeto de muita pesquisa e especulação, mas de pouca ação. O medo de efeitos contrários e dúvidas sobre sua eficiência como armas devem ter limitado seu uso. Recentemente, se verificou uma nova forma de terrorismo, empregando agentes infecciosos, devagar e sem muito alarde, até as ocorrências recentes com o Bacillus anthracis nos Estados Unidos. A varíola é possivelmente o mais devastador desses agentes. Menos de 25 anos passados desde sua erradicação, a saúde pública tem que lidar com a possibilidade de sua re-introdução. O cenário da re-introdução da varíola no Brasil é discutido.

  15. Guerra biológica, bioterrorismo e saúde pública

    Directory of Open Access Journals (Sweden)

    Luiz Jacintho da Silva

    2001-12-01

    Full Text Available O uso de agentes biológicos como arma não é novidade para a humanidade. Durante séculos, até a atualidade, a guerra biológica tem sido objeto de muita pesquisa e especulação, mas de pouca ação. O medo de efeitos contrários e dúvidas sobre sua eficiência como armas devem ter limitado seu uso. Recentemente, se verificou uma nova forma de terrorismo, empregando agentes infecciosos, devagar e sem muito alarde, até as ocorrências recentes com o Bacillus anthracis nos Estados Unidos. A varíola é possivelmente o mais devastador desses agentes. Menos de 25 anos passados desde sua erradicação, a saúde pública tem que lidar com a possibilidade de sua re-introdução. O cenário da re-introdução da varíola no Brasil é discutido.

  16. Efectos biológicos de los stents medicados en la circulación coronaria

    Directory of Open Access Journals (Sweden)

    Darío Echeverri, MD

    2010-03-01

    Full Text Available Los stents medicados ofrecen la mejor alternativa disponible no quirúrgica para el tratamiento de la enfermedad coronaria gracias a su demostrada eficacia. Sin embargo, estos excelentes resultados han sido opacados en términos de seguridad, principalmente por la presencia de trombosis de stents de manera tardía o muy tardía. La comprensión de los efectos biológicos que ejercen a nivel de la arteria coronaria luego de su implante, se debe al efecto de la plataforma utilizada, el polímero y la droga que liberan. Los trastornos de reparación vascular inducidos favorecen la trombosis de stents y sus consecuencias clínicas. Se hace una revisión de los diferentes efectos biológicos de los stents medicados en las arterias coronarias, que permite comprender como han surgido rápidamente nuevas versiones en materiales, diseños, polímeros y medicamentos que reducen los efectos adversos a nivel coronario, mejorando su eficacia y seguridad.

  17. Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice.

    Science.gov (United States)

    Nakahara, Mai; Tateyama, Hiroki; Araki, Masatake; Nakagata, Naomi; Yamamura, Ken-ichi; Araki, Kimi

    2013-06-01

    The MSM/Ms strain is derived from the Japanese wild mouse Mus musculus molossinus and displays characteristics not observed in common laboratory strains. Functional genomic analyses using genetically engineered MSM/Ms mice will reveal novel phenotypes and gene functions/interactions. We previously reported the establishment of a germline-competent embryonic stem (ES) cell line, Mol/MSM-1, from the MSM/Ms strain. To analyze its usefulness for insertional mutagenesis, we performed gene-trapping using these cells. In the present study, we compared the gene-trap events between Mol/MSM-1 and a conventional ES cell line, KTPU8, derived from the F1 progeny of a C57BL/6 × CBA cross. We introduced a promoter-trap vector carrying the promoterless β-galactosidase/neomycin-resistance fusion gene into Mol/MSM-1 and KTPU8 cells, isolated clones, and identified the trapped genes by rapid amplification of cDNA 5'-ends (5'-RACE), inverse PCR, or plasmid rescue. Unexpectedly, the success rate of 5'-RACE in Mol/MSM trap clones was 47 %, lower than the 87 % observed in KTPU8 clones. Genomic analysis of the 5'-RACE-failed clones revealed that most had trapped ribosomal RNA gene regions. The percentage of ribosomal RNA region trap clones was 41 % in Mol/MSM-1 cells, but less than 10 % in KTPU8 cells. However, within the Mol/MSM-1 5'-RACE-successful clones, the trapping frequency of annotated genes, the chromosomal distribution of vector insertions, the frequency of integration into an intron around the start codon-containing exon, and the functional spectrum of trapped genes were comparable to those in KTPU8 cells. By selecting 5'-RACE-successful clones, it is possible to perform gene-trapping efficiently using Mol/MSM-1 ES cells and promoter-trap vectors.

  18. Autotaxin : biochemical and functional studies

    NARCIS (Netherlands)

    Houben, Anna Jacoba Sara

    2012-01-01

    This thesis focuses on autotaxin (ATX), the main enzyme responsible for the production of lysophosphatidic acid (LPA). The ATX-LPA receptor axis has a wide implication in health and disease. The studies described in this thesis aim at characterizing the biochemical and functional properties of ATX,

  19. Serum biochemical changes accompanying prolonged ...

    African Journals Online (AJOL)

    Jane

    2010-10-18

    Oct 18, 2010 ... Toxicological evaluation of the whole fruit of Lagenaria breviflora was carried out using the serum biochemical changes accompanying prolonged administration of the ethanolic extract of the fruit in ... glucose in rats administered with the extract of 8000 mg/kg body weight increased two and half-fold.

  20. Dietary fibre concentrate from Chilean algarrobo (Prosopis chilensis (Mol.) Stuntz) pods: purification and characterization.

    Science.gov (United States)

    Estévez, Ana María; Figuerola, Fernando; Bernuy, Enrique; Sáenz, Carmen

    2014-12-01

    Prosopis species are generally fast-growing, drought-resistant, nitrogen-fixing trees or shrubs. Fruits of Prosopis spp are indehiscent pods, where pericarp is formed by the epicarp, light brown in colour, and fibrous nature; the mesocarp known as pulp, which is rich in sugars; and the endocarp. The aim of this work was to obtain a fibre concentrate from the pods of Prosopis chilensis Mol. (Stuntz) and to determine the chemical, physical, and technological properties of the pod flour (PF) and of a fibre concentrate or pod purified flour (PPF). Acetone, ethanol, and water at different conditions of time and temperature were used in the purification process. PF showed 53.7 g/100 g of total sugar content, 4.2 g/100 g of reducing sugar content, 41.8 g/100 g of total dietary fibre, 35.8 g/100 g of insoluble fibre, and 6.0 g/100 g of soluble fibre content. The PPF has a total sugar content of 3.8 g/100 g, reducing sugar content of 2.2 g/100 g, total dietary fibre content of 80.8 g/100 g, insoluble fibre content of 75.1 g/100 g, and soluble fibre content of 5.7 g/100 g. The scanning electron microscopy analysis showed the existence of voids in the structure of PPF flour, which reveals the efficiency of the purification process with a high decrease in the total sugar content. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  1. ExoMol molecular line lists - XXIII. Spectra of PO and PS

    Science.gov (United States)

    Prajapat, Laxmi; Jagoda, Pawel; Lodi, Lorenzo; Gorman, Maire N.; Yurchenko, Sergei N.; Tennyson, Jonathan

    2017-12-01

    Comprehensive line lists for phosphorus monoxide (31P16O) and phosphorus monosulphide (31P32S) in their X 2Π electronic ground state are presented. The line lists are based on new ab initio potential energy (PEC), spin-orbit (SOC) and dipole moment (DMC) curves computed using the MRCI+Q-r method with aug-cc-pwCV5Z and aug-cc-pV5Z basis sets. The nuclear motion equations (i.e. the rovibronic Schrödinger equations for each molecule) are solved using the program DUO. The PECs and SOCs are refined in least-squares fits to available experimental data. Partition functions, Q(T), are computed up to T = 5000 K, the range of validity of the line lists. These line lists are the most comprehensive available for either molecule. The characteristically sharp peak of the Q-branches from the spin-orbit split components gives useful diagnostics for both PO and PS in spectra at infrared wavelengths. These line lists should prove useful for analysing observations and setting up models of environments such as brown dwarfs, low-mass stars, O-rich circumstellar regions and potentially for exoplanetary retrievals. Since PS is yet to be detected in space, the role of the two lowest excited electronic states (a 4Π and B 2Π) are also considered. An approximate line list for the PS X-B electronic transition, which predicts a number of sharp vibrational bands in the near ultraviolet, is also presented. The line lists are available from the CDS (http://cdsarc.u-strasbg.fr) and ExoMol (www.exomol.com) data bases.

  2. Use of continuous lactose fermentation for ethanol production by Kluveromyces marxianus for verification and extension of a biochemically structured model

    DEFF Research Database (Denmark)

    Sansonetti, S.; Hobley, Timothy John; Curcio, S.

    2013-01-01

    A biochemically structured model has been developed to describe the continuous fermentation of lactose to ethanol by Kluveromyces marxianus and allowed metabolic coefficients to be determined. Anaerobic lactose-limited chemostat fermentations at different dilution rates (0.02 – 0.35 h-1) were...... performed. Species specific rates of consumption/formation, as well as yield coefficients were determined. Ethanol yield (0.655 C-mol ethanol*C-mol lactose-1) was as high as 98 % of theoretical. The modeling procedure allowed calculation of maintenance coefficients for lactose consumption and ethanol...

  3. Localization and quantification of hydrophobicity: The molecular free energy density (MolFESD) concept and its application to sweetness recognition

    Science.gov (United States)

    Jäger, Robert; Schmidt, Friedemann; Schilling, Bernd; Brickmann, Jürgen

    2000-10-01

    A method for the localization, the quantification, and the analysis of hydrophobicity of a molecule or a molecular fragment is presented. It is shown that the free energy of solvation for a molecule or the transfer free energy from one solvent to another can be represented by a surface integral of a scalar quantity, the molecular free energy surface density (MolFESD), over the solvent accessible surface of that molecule. This MolFESD concept is based on a model approach where the solvent molecules are considered to be small in comparison to the solute molecule, and the solvent can be represented by a continuous medium with a given dielectric constant. The transfer energy surface density for a 1-octanol/water system is empirically determined employing a set of atomic increment contributions and distance dependent membership functions measuring the contribution of the increments to the surface value of the MolFESD. The MolFESD concept can be well used for the quantification of the purely hydrophobic contribution to the binding constants of molecule-receptor complexes. This is demonstrated with the sweeteners sucrose and sucralose and various halogen derivatives. Therein the relative sweetness, which is assumed to be proportional to the binding constant, nicely correlates to the surface integral over the positive, hydrophobic part of the MolFESD, indicating that the sweetness receptor can be characterized by a highly flexible hydrophobic pocket instead of a localized binding site.

  4. Corrosion inhibition of iron in 0.5 mol L-1 H2SO4 by halide ions

    Directory of Open Access Journals (Sweden)

    Jeyaprabha C.

    2006-01-01

    Full Text Available The inhibition effect of halide ions such as iodide, bromide and chloride ions on the corrosion of iron in 0.5 mol L-1 H2SO4 and the adsorption behaviour of these ions on the electrode surface have been studied by polarization and impedance methods. It has been found that the inhibition of nearly 90% has been observed for iodide ions at 2.5 10-3 mol L-1, for bromide ions at 10 10-3 mol L-1 and 80% for chloride ions at 2.5 10-3 mol L-1. The inhibition effect is increased with increase of halide ions concentration in the case of I- and Br- ions, whereas it has decreased in the case of Cl- ion at concentrations higher than 5 10-3 mol L-1. The double layer capacitance values have decreased considerably in the presence of halide ions which indicate that these anions are adsorbed on iron at the corrosion potential.

  5. Magnétisme des cristaux de molécules à grand spin

    Science.gov (United States)

    Villain, J.

    2003-01-01

    outlined. A last appendix contains an index. Les chimistes ont créé, dans les dernières années du vingtième siècle, des molécules magnétiques dont le spin (typiquement s=10) est suffisamment grand pour que le temps de relaxation de l'aimantation puisse devenir macroscopique à basse température. Ces nouveautés donnent lieu à des phénomènes nouveaux dont nous donnons ici une description détaillée. Nous commençons par rappeler les concepts fondamentaux du magnétisme, tels que l'anisotropie et l'échange, puis nous décrivons les molécules à grand spin et nous les situons dans le monde du magnétisme. Dans le deuxième chapitre, nous étudions la relaxation magnétique thermiquement activée, en utilisant l'équation maîtresse et en évaluant ses coefficients à partir de l'interaction spin-phonons et de la règle d'or. Le troisième chapitre est consacré à l'effet tunnel d'un spin isolé, et nous calculons l'écart tunnel par des méthodes simples. Nous obtenons en particulier les valeurs du champ magnétique où la séparation tunnel s'annule (points "diaboliques"). Dans le quatrième chapitre, nous décrivons brièvement des méthodes alternatives basées sur les intégrales sur les chemins. Dans le cinquième chapitre, nous étudions l'effet, toujours sur un spin isolé, d'un champ magnétique dépendant du temps, qui déclenche le retournement partiel des spins au moment où la valeur du champ autorise l'effet tunnel. Le sixième chapitre est consacré à l'interaction du spin avec son environnement à basse température, quand la relaxation magnétique est due aux interactions dipolaires avec les autres spins moléculaires et avec les spins nucléaires. Dans le septième et dernier chapitre, nous traitons l'effet tunnel du spin entre états excités, phénomène original et important dans ces matériaux. Enfin, dans six annexes, nous rappelons les constantes fondamentales et les différentes notations courantes, la correspondance entre les divers

  6. Tratamiento biológico aerobio de alpechín depurado previamente con ozono

    Directory of Open Access Journals (Sweden)

    Domínguez Vargas, Joaquín R.

    2000-10-01

    Full Text Available In the present work, the degradation of olive mill wastewaters previously treated by an ozonation stage has been studied by an aerobic biological oxidation. The substrate evolution (based on chemical oxygen demand, biomass (measured as volatile suspended solids and total polyphenolic contents were followed during each experiment. A kinetic study is performed by using the Contois model, which applied to the experimental data, provides the specific kinetic parameters of this model. The deduced kinetic equation for the consume of substrate is q= 17.0 S/(18.2 X + S0 X g COD/ gVSS. day. Moreover, others interesting biological parameters like the cellular yield coefficient and the kinetic rate constant for the endogenous metabolism were determined, obtaining a values of 0.214 g VSS/g COD and 0.167 day-1, respectively.En el presente trabajo se ha estudiado la oxidación biológica aerobia por Iodos activos de las aguas residuales de almazaras (alpechín que previamente habían sido depuradas por una etapa química mediante ozono. La evolución del proceso biológico fue seguida midiendo la concentración de sustrato (en términos de demanda química de oxígeno, la concentración de biomasa (medida como sólidos en suspensión volátiles y el contenido en compuestos polifenólicos totales. Se realiza un estudio cinético basado en el modelo de Contois que, aplicado a los datos experimentales, permite calcular los parámetros cinéticos específicos de este modelo. La ecuación deducida para el consumo de sustrato es q= 17.0 S/(18.2 X + S0 X, g DQO/ gSSV. día. Al mismo tiempo se han determinado otros parámetros biológicos de interés como son el coeficiente de rendimiento celular y la constante cinética correspondiente al metabolismo endógeno, obteniéndose unos valores de 0.214 g SSV/g DQO y 0.167 día-1, respectivamente.

  7. Evaluación de riesgo biológico en el Hospital Rey Don Jaime

    Directory of Open Access Journals (Sweden)

    Salomé Benavent Nácher

    2007-03-01

    Full Text Available Objetivo: El propósito del presente trabajo es realizar una evaluación del riesgo biológico existente para los trabajadores del Hospital con el fin de identificar los puestos de trabajo y microorganismos que suponen mayor riesgo y poder adoptar medidas preventivas. Métodos: Se aplicó la metodología BIOGAVAL de evaluación de riesgo biológico desarrollado por el Gabinete de Seguridad e Higiene de Valencia. Dicho método contempla una identificación de los microorganismos presentes mas probables, su posible daño a la salud, mecanismos de transmisión, porcentaje de población diana vacunado y el efecto protector de las medidas higiénicas. Se aplicó a los distintos puestos de trabajo que presentan riesgo biológico exceptuando cocina. Resultados: Los resultados evidencian un mayor riesgo para los microorganismos de transmisión aérea y con baja protección vacunal, así como un nivel de riesgo superior (comparado con otros trabajadores de los ATS/DUE de urgencias. Conclusiones: El método se ha revelado útil para evidenciar los puestos de trabajo más peligrosos así como los agentes microbiológicos de mayor riesgo. Igualmente aporta una orientación sobre la prioridad para la aplicación de medidas preventivas de control.Objective: The purpose of this work is to realize an evaluation of the existing biological risk for the staff at the hospital with the aim of both identifying the jobs and microorganisms which mean a higher risk and to be able to adopt preventive measures. Methods: It was applied the BIOGAVAL methodology of evaluation of biological risk developed by the Safety and Hygiene Office in Valencia. This method deals with an identification of the most probable present microorganisms, their possible hurt for health, transmission mechanisms, percentage of target population vaccinated and the protective effect of the hygienic measures. It was applied to the different jobs which show biological risk except for the kitchen ones

  8. La Evolución biológica en la Biofilosofía de F. J. Ayala

    OpenAIRE

    Diego Cano Espinosa

    2016-01-01

    El discurso biológico de Ayala se centra en estas tres cuestiones: 1) El hecho de la evolución. 2) Historia de la evolución. 3) Mecanismos del desarrollo y cambio evolutivos. El hecho determinante y cierto, según Ayala, es la evidencia de relaciones entre todos los organismos debidas a una común descendencia con modificaciones como lo han demostrado con abundancia la Paleontología, Anatomía comparada, Biogeografía, Embriología, Genética Molecular, Bioinformática y otras disciplinas biológicas...

  9. Contribuições a estudos biológicos com o uso de modelos biofísicos

    OpenAIRE

    de Cássia Moura do Nascimento, Rita

    2004-01-01

    Um modelo biofísico é uma representação simplificada e/ou abstrata de processos ou sistemas biológicos. Objetivando ampliar o conhecimento sobre a modelagem biofísica, esta Tese enfoca prioritariamente os modelos que desenvolvemos, visando contribuir com os estudos biológicos. Proteínas transportadoras do tipo canal iônico encontram-se presentes na membrana plasmática de todos os seres vivos e o primeiro modelo biofísico é uma membrana plasmática artificial, na qual há um co...

  10. Investigación sobre el efecto de la temperatura en los procesos biológicos por fangos activos

    OpenAIRE

    Francisco Díaz, Juan Pablo de

    2011-01-01

    Durante la presente Investigación se ha estudiado el efecto que produce la temperatura en los procesos de depuración biológica por fangos activos, desarrollando la influencia de dicho factor en cada una de las etapas de la línea de agua de una estación depuradora. - Pretratamiento - Decantación Primaria - Reactor Biológico - Decantación Secundaria Se ha considerado que los efectos de la temperatura en los procesos meramente físicos, son mínimos, únicamente provocados por la variación de la de...

  11. Avaliação de bactérias nitrificantes em filtros biológicos submersos aerados

    OpenAIRE

    Delgado, Tatiana Cardoso

    2009-01-01

    Neste estudo foi pesquisado um novo sistema de tratamento de efluente com filtros biológicos anaeróbios e aeróbios, visando a transformação das formas de nitrogênio. O principal objetivo deste trabalho foi avaliar, em escala piloto, quantitativamente e qualitativamente a comunidade bacteriana nitrificante em um sistema de tratamento de esgoto experimental composto por filtros biológicos submersos aerados em série, para compreender a dinâmica no processo de transformação de nitrogênio. Para...

  12. Simulación numérica del crecimiento hipertrófico de tejidos biológicos

    OpenAIRE

    Bellomo, Facundo; Oller Martínez, Sergio Horacio; Nallim, Liz

    2012-01-01

    El crecimiento de los tejidos biológicos blandos tiene un papel fundamental en una serie de procesos normales y patológicos de gran incidencia en la salud humana. Algunos de los factores que regulan estos procesos en los tejidos biológicos son fundamentalmente mecánicos, de allí la importancia del estudio de estos fenómenos en el marco de una formulación que permita estimar los campos de tensión y deformación, así como la interrelación con los fenómenos de crecimiento. En general, los mode...

  13. Control biológico de moscas blancas en cultivo de tomate: interacciones entre sus enemigos naturales

    OpenAIRE

    Moreno Ripoll, Rafael

    2011-01-01

    [spa] Para obtener un control biológico adecuado de las plagas presentes en un cultivo es importante determinar las relaciones tróficas que existen entre los enemigos naturales y su efecto sobre el control de estas plagas. Dos de estas interacciones, la depredación intragremial y el canibalismo, pueden condicionar el éxito de los programas de control biológico produciendo efectos aditivos o negativos sobre las poblaciones de la plaga. Para determinar la ingesta de todos los estados de desarro...

  14. Moléstia de Charcot-Marie-Tooth: conceito clínico-patológico atual

    Directory of Open Access Journals (Sweden)

    José Antonio Levy

    1962-06-01

    Full Text Available Após considerações sobre o quadro anátomo-patológico da moléstia de Charcot-Marie-Tooth, o autor relata 4 casos, chegando à conclusão de que esta moléstia pode ser considerada como uma neuromiopatia em virtude de algumas alterações histopatológicas musculares serem primárias, ao passo que outras são secundárias à lesão do neurônio motor periférico.

  15. Étude des matrices sol-gel en film mince par détection de molécule unique en champ large : diffusion moléculaire et agrégation photo-induite

    Science.gov (United States)

    Nutarelli, D.; Débarre, A.; Jaffiol, R.; Julien, C.; Richard, A.; Tchénio, P.; Chaput, F.; Boilot, J. P.

    2004-11-01

    La fluorescence de molécules de colorant dans des matrices sol-gel en film mince a été étudiée par détection de molécule unique. Outre les phénomènes de photo-blanchiment et de clignotement, un processus de photo-agrégation a été observé. Ce phénomène, dont la dynamique dépend de la présence d'oxygène, de l'intensité du laser d'excitation et de la température, montre l'existence de deux classes de molécules dans l'échantillon. Des molécules immobiles et d'autres qui diffusent. L'analyse de la dynamique d'agrégation permettra de dégager certaines caractéristiques du mécanisme de photo-agrégation et d'appréhender le rôle joué par la matrice sol-gel dans ce processus.

  16. Satisfação conjugal e parentalidade biológica e adoptiva

    OpenAIRE

    Ferreira, Júlia Maria de Araújo

    2008-01-01

    Tese de mestrado, Psicologia (Psicologia Clínica e da Saúde - Núcleo de Psicologia Clínica Sistémica), 2008, Universidade de Lisboa, Faculdade de Psicologia e de Ciências da Educação O presente estudo, pretendendo uma comparação entre famílias portuguesas biológicas e adoptivas, tem como objectivo analisar a percepção de Satisfação Conjugal e a sua respectiva influência nos Estilos Parentais praticados, com o intuito de compreender a teia de relações existente entre os domínios da Conjugal...

  17. Estudo fitoquímico e biológico de Vernonia tweediana Baker (Asteraceae)

    OpenAIRE

    Díaz, Gaspar; Nogueira, Marisa A.; Olguín, Conceição F. A.; Somensi, Andressa; Vidotti, Gentil J.

    2008-01-01

    A mistura de α-amirina, β-amirina e lupeol e o ácido palmítico puro foram os principais constituintes químicos isolados do extrato clorofórmio da parte aérea de V. tweediana. Lupenil palmitato puro e a mistura de α-amiril palmitato e β-amiril palmitato foram isolados a partir do extrato clorofórmio das raízes. As estruturas foram caracterizadas por métodos químicos e espectroscópicos. Também foram investigadas as atividades biológicas dos extratos hexano, clorofórmio, acet...

  18. De la riqueza biológica a la riqueza económica

    Directory of Open Access Journals (Sweden)

    Gustavo Correa Assmus

    2011-01-01

    Full Text Available Frente al deterioro ambiental por efectos de la producción y el consumo, la economía debe ofrecer respuestas fundamentadas en procesos de investigación, educación, responsabilidad y regulación, que permitan articular el aprovechamiento de los recursos naturales renovables, administrados y no administrados, con el fin de establecer un crecimiento económico-ambiental sostenible y racionalizado que cualifique la calidad de vida. Para esta propuesta se tomó información secundaria de origen empírico, la cual permitió elaborar una teorización analítica, que expresa de manera clara sus reflexiones y conjeturas, ofreciendo un camino por seguir en el proceso de transición de la riqueza biológica a la económica.

  19. Bioseguridad y armas biológicas:\tLa amenaza de Andrómeda (1971

    Directory of Open Access Journals (Sweden)

    Manuel SÁNCHEZ

    2016-11-01

    Full Text Available La amenaza de Andrómeda es la adaptación cinematográfica de la obra homónima escrita por Michael Crichton. Puede considerarse como la película más “microbiológica” del Séptimo Arte. Dirigida por Robert Wise, este tecno-thriller describe los esfuerzos de un equipo de científicos para caracterizar y contener a un microorganismo patógeno extraterrestre. A pesar de haber sido realizada hace 40 años, se tratan temas de plena actualidad como es el de las armas biológicas, las medidas de aislamiento y protección, la automatización en los procedimientos sanitarios y la toma de decisiones ante una situación grave e inesperada.

  20. Bioética e armas biológicas no contexto internacional

    OpenAIRE

    Tapajós, Ana Maria

    2011-01-01

    O estudo “Bioética e Armas Biológicas no Contexto Internacional” investiga a aplicação de análise sob a ótica da bioética de intervenção a diálogos internacionais no âmbito de um sistema de estados. O estudo de caso testa o método de investigação que consiste na identificação de valores e posicionamentos contratantes entre países desenvolvidos e em desenvolvimento e suas possibilidades de incorporação a regras, normas e regulações adotadas pela comunidade internacional. Nesse processo é ve...

  1. Turismo en espacios naturales: oportunidades en el corredor biológico mesoamericano

    Directory of Open Access Journals (Sweden)

    Miguel Panadero Moya

    2002-01-01

    Full Text Available El Corredor Biológico Mesoamericano es una iniciativa de los presidentes de los Estados centroamericanos para facilitar la integración subregional mediante estrategias fundamentadas en la singularidad e importancia ecológica de sus recursos naturales. Pretende implantar medidas que aseguren la conservación de estos recursos de forma compatible con el aprovechamiento socioeconómico de su potencial turístico. El territorio que lo integra se extiende por todo el istmo centroamericano y posee notables recursos naturales de alto valor ecológico y zonas de interés histórico-artístico con gran importancia cultural. Sus elementos, que se engarzan en una cadena de verdes eslabones de singular vegetación, constituyen reductos de indudable atractivo y aptitud para la práctica del turismo en la naturaleza. La incorporación a los circuitos internacionales de viajeros de este proyecto está limitada por las precarias características socioeconómicas de sus pobladores y por la debilidad de las infraestructuras y equipamientos turísticos de la mayoría de los países implicados en el proyecto. Crear un pasillo biológico en armonía con sus habitantes, en uno de los esfuerzos de conservación para el desarrollo más grande planteado hasta el momento, es la idea de sus promotores que, con esta iniciativa, se muestran participes de la lógica de la globalización.

  2. Terapia biológica en enfermedades reumatológicas

    Directory of Open Access Journals (Sweden)

    Manuel F Ugarte-Gil

    2013-04-01

    Full Text Available El advenimiento del uso de terapias biológicas en Reumatología ha modificado significativamente el pronóstico de pacientes portadores de artritis reumatoide (AR, artritis juvenil (AJ, espondilitis anquilosante (EA, entre otras enfermedades. A diferencia de las terapias convencionales estos productos biológicos se dirigen a los llamados blancos terapéuticos ya sea estas una línea celular, un mediador inflamatorio o un receptor de superficie. Estos compuestos son producidos por células vivas mediante la tecnología del ADN recombinante. Estos compuestos pueden tener componentes humano y animal [quiméricos (Xi, humanizados (Zu], o completamente humanos (H lo cual se reconoce por las letras que se incluyen en el nombre de cada uno. En el campo de la Reumatología, el primer compuesto utilizado fue el etanercept (anti-factor de necrosis tumoral o anti-TNF aprobado en 1998, pero otros anti-TNF han demostrado su beneficio en AR, como en EA y AJ. Los inhibidores de Interleucina (IL-1 casi no se usan en AR actualmente, pero si los inhibidores de IL-6, así como los agentes contra las células B y los agonistas de CTLA-4 (Cytotoxic T lymphocyte antigen. Existe asimismo un compuesto dirigido al BLyS (B-lymphocyte stimulator el cual se usa en lupus eritematoso sistémico y otro dirigido al receptor activador del factor nuclear κB (RANKL, receptor activator of nuclear factor-κB ligand que se usa en osteoporosis. Con el avance en el conocimiento de la patogenia de las enfermedades reumαticas, se vienen reconociendo otra blancos terapιuticas. En los aρos venideros, este campo ha de expandirse en proporciones geomιtricas

  3. Aislamiento de microorganismos para control biológico de Moniliophthora roreri

    Directory of Open Access Journals (Sweden)

    Liliana Yanet Suárez Contreras

    2013-12-01

    Full Text Available Moniliophthora roreri es un hongo que causa la moniliasis, una enfermedad de la mazorca en el cultivo de cacao (Theobroma cacao, con pérdidas hasta del 60% de la cosecha. El control biológico utilizando microorganismos endófitos surge como una alternativa para el manejo de esta enfermedad. En la presente investigación se evaluaron microorganismos con potencial para control biológico de M. roreri en Norte de Santander, Colombia. Para el efecto, se aisló e identificó este fitopatógeno y se utilizaron protocolos de desinfección de los posibles microorganismos antagonistas con siembras por diluciones seriadas, selección de los géneros microbianos con mayor potencial antagónico y evaluación de las cepas por la prueba de plato dual para evaluar el efecto biocontrolador de los hongos y la antibiosis para bacterias. Se tomaron muestras en los municipios de Cúcuta, Sardinata, El Tarra, Tibú y El Zulia, de las cuales se aislaron 17 cepas del fitopatógeno y 20 entre hongos y bacterias. De éstas se seleccionaron cuatro cepas de hongos y tres de bacterias por su capacidad antagónica contra M. roreri. Los mejores porcentajes de inhibición de crecimiento radial (PICR se alcanzaron con Paecilomyces sp. (HC002 vs M. roreri, con una media de 80.72%, seguido del tratamiento con Paecilomyces sp. (HZ002 vs M. roreri con 79.45%. Se demostró que el hongo Paecilomyces sp. también tiene un alto potencial antagónico in vitro frente a M. roreri. Al evaluar la antibiosis de las bacterias aisladas, se encontró que Bacillus brevis (BZ005 fue la más efectiva en todos los sitios del estudio, con porcentajes superiores a 89%.

  4. Possível parentesco entre as moléstias familiares com lesões predominantes na medula e moléstias familiares com lesões no neurônio periférico

    Directory of Open Access Journals (Sweden)

    Paulino W. Longo

    1943-06-01

    Full Text Available Os AA., depois de descreverem em linhas gerais as características da atrofia muscular progressiva e familiar tipo Charcot-Marie-Tooth, assinalam suas semelhanças com as atrofias musculares tipo Dejerine-Sottas e Werdnig-Hoffmann. Chamam atenção a seguir para a tendência, aliás já antiga de se reunir as moléstias familiares clássicas em um só grupo, pois as formas de transição entre umas e outras são bastante frequentes. A êsse respeito lembram que Austregésilo, desde 1918, defendeu essa teoria com abundância de documentação, procurando reuni-las em tôrno do que êle chama grande família hipotrófica cuja entidade central é a moléstia de Friedreich. A contribuição que trazem ao assunto é a observação de tres irmãos, membros de uma família em cujos ancestrais já havia sido assinalada paraplegia progressiva, e que apresentam o quadro neurológico típico da moléstia Charcot-Marie-Tooth, acrescido, num deles, de um síndromo piramidal bilateral (reflexos profundos exaltados, clonus da rótula e pés, sinal de Babinski e em outro, de exaltação dos reflexos patelares. Esta associação, em uma moléstia familiar degenerativa do neurônio periférico, de um quadro degenerativo cordonal, é mais um argumento favorável à unidade nosológica de todas estas moléstias familiares sistematizadas.

  5. Biochemical markers of bone turnover

    International Nuclear Information System (INIS)

    Kim, Deog Yoon

    1999-01-01

    Biochemical markers of bone turnover has received increasing attention over the past few years, because of the need for sensitivity and specific tool in the clinical investigation of osteoporosis. Bone markers should be unique to bone, reflect changes of bone less, and should be correlated with radiocalcium kinetics, histomorphometry, or changes in bone mass. The markers also should be useful in monitoring treatment efficacy. Although no bone marker has been established to meet all these criteria, currently osteocalcin and pyridinium crosslinks are the most efficient markers to assess the level of bone turnover in the menopausal and senile osteoporosis. Recently, N-terminal telopeptide (NTX), C-terminal telopeptide (CTX) and bone specific alkaline phosphatase are considered as new valid markers of bone turnover. Recent data suggest that CTX and free deoxypyridinoline could predict the subsequent risk of hip fracture of elderly women. Treatment of postmenopausal women with estrogen, calcitonin and bisphosphonates demonstrated rapid decrease of the levels of bone markers that correlated with the long-term increase of bone mass. Factors such as circadian rhythms, diet, age, sex, bone mass and renal function affect the results of biochemical markers and should be appropriately adjusted whenever possible. Each biochemical markers of bone turnover may have its own specific advantages and limitations. Recent advances in research will provide more sensitive and specific assays

  6. Biochemical markers of bone turnover

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Deog Yoon [College of Medicine, Kyunghee Univ., Seoul (Korea, Republic of)

    1999-08-01

    Biochemical markers of bone turnover has received increasing attention over the past few years, because of the need for sensitivity and specific tool in the clinical investigation of osteoporosis. Bone markers should be unique to bone, reflect changes of bone less, and should be correlated with radiocalcium kinetics, histomorphometry, or changes in bone mass. The markers also should be useful in monitoring treatment efficacy. Although no bone marker has been established to meet all these criteria, currently osteocalcin and pyridinium crosslinks are the most efficient markers to assess the level of bone turnover in the menopausal and senile osteoporosis. Recently, N-terminal telopeptide (NTX), C-terminal telopeptide (CTX) and bone specific alkaline phosphatase are considered as new valid markers of bone turnover. Recent data suggest that CTX and free deoxypyridinoline could predict the subsequent risk of hip fracture of elderly women. Treatment of postmenopausal women with estrogen, calcitonin and bisphosphonates demonstrated rapid decrease of the levels of bone markers that correlated with the long-term increase of bone mass. Factors such as circadian rhythms, diet, age, sex, bone mass and renal function affect the results of biochemical markers and should be appropriately adjusted whenever possible. Each biochemical markers of bone turnover may have its own specific advantages and limitations. Recent advances in research will provide more sensitive and specific assays.

  7. Physiological and biochemical responses of halophyte Kalidium ...

    African Journals Online (AJOL)

    In this study, the physiological and biochemical responses of a halophyte Kalidium foliatum to salinity were studied. In order to reflect salt-tolerance in K. foliatum and to analyze the physiological and biochemical mechanism for its salt tolerance, salinity threshold and biochemical parameters were studied. A halophyte ...

  8. Evaluation Of Method Of Line (MOL For Solution Of Soil Water Flow Equations And Comparison With Finite Element Method (FEM

    Directory of Open Access Journals (Sweden)

    ISAM M. Mahammed

    2012-03-01

    Full Text Available The objective of this research is to evaluate Method of Line (MOL used for solution of  water flow equations through porous media using MATLAB package functions for solution of ordinary differential equations ODE,s  , instead of writing long programs codes.  sink & source term to MOL model were included. Then Comparing MOL model with another model that uses finite element method in solving water flow equations (FEM in one dimensional flow using computer program code in FORTRAN.  Two cases were examined for evaluation and comparison of these two models. Firstly, infiltration phenomena using sandy soil was studied with the same parameter for both models.  Results show that there is a divergence between the two models along time of 60 minutes of infiltration. Changes of moisture content with soil depth were sharp with FEM model. Second case, data of  the volume  of water content for wheat field where used taking irrigation and evaporation into account, along the growth period of wheat crop and different depths up to  100 cm. Results show that output of  FEM model has  high degree of agreement with  the measured data for all depths and along all period of growth. Data given by MOL model were less in values than measured data for all depths and along all period of wheat growth time.

  9. Sintering behaviour and microstructure of 3Y-TZP + 8 mol% CuO nano-powder composite

    NARCIS (Netherlands)

    Ran, S.; Winnubst, Aloysius J.A.; Koster, H.; de Veen, P.J.; Blank, David H.A.

    2007-01-01

    Nanocrystalline 3Y-TZP and copper-oxide powders were prepared by co-precipitation of metal chlorides and copper oxalate complexation– precipitation, respectively.Asignificant enhancement in sintering activity of 3Y-TZP nano-powders, without presence of liquid phase,was achieved by addition of 8 mol%

  10. MolWorks ─統合型分子設計支援システム─

    OpenAIRE

    田島 澄恵

    2015-01-01

    MolWorks は,分子設計を統合的に支援することを目的に開発されたソフトウェアである。MolWorks には,分子モデリング,量子化学計算支援,化学工学計算,物性推算などの機能が含まれる。本論文では,これらの機能の詳細について述べる。MolWorks は,科学分野を専攻する学生や研究者向けに作られてきており,全て英語メニューとなっている。MolWorks の日本語版を開発し,小中高生でも,楽しく気軽に分子や物性を学べる教材を提供していきたい。...

  11. Biochemical adaptation to ocean acidification.

    Science.gov (United States)

    Stillman, Jonathon H; Paganini, Adam W

    2015-06-01

    The change in oceanic carbonate chemistry due to increased atmospheric PCO2  has caused pH to decline in marine surface waters, a phenomenon known as ocean acidification (OA). The effects of OA on organisms have been shown to be widespread among diverse taxa from a wide range of habitats. The majority of studies of organismal response to OA are in short-term exposures to future levels of PCO2 . From such studies, much information has been gathered on plastic responses organisms may make in the future that are beneficial or harmful to fitness. Relatively few studies have examined whether organisms can adapt to negative-fitness consequences of plastic responses to OA. We outline major approaches that have been used to study the adaptive potential for organisms to OA, which include comparative studies and experimental evolution. Organisms that inhabit a range of pH environments (e.g. pH gradients at volcanic CO2 seeps or in upwelling zones) have great potential for studies that identify adaptive shifts that have occurred through evolution. Comparative studies have advanced our understanding of adaptation to OA by linking whole-organism responses with cellular mechanisms. Such optimization of function provides a link between genetic variation and adaptive evolution in tuning optimal function of rate-limiting cellular processes in different pH conditions. For example, in experimental evolution studies of organisms with short generation times (e.g. phytoplankton), hundreds of generations of growth under future conditions has resulted in fixed differences in gene expression related to acid-base regulation. However, biochemical mechanisms for adaptive responses to OA have yet to be fully characterized, and are likely to be more complex than simply changes in gene expression or protein modification. Finally, we present a hypothesis regarding an unexplored area for biochemical adaptation to ocean acidification. In this hypothesis, proteins and membranes exposed to the

  12. [The nuclear matrix proteins (mol. mass 38 and 50 kDa) are transported by chromosomes in mitosis].

    Science.gov (United States)

    Murasheva, M I; Chentsov, Iu S

    2010-01-01

    It was shown by immunofluorescence method that serum M68 and serum K43 from patients with autoimmune disease stain interphase nuclei and periphery of mitotic chromosomes of pig kidney cells. Western blotting reveals the polypeptide with mol. mass of 50 kDa in serum M68, and the polypeptide with mol. mass of 38 kDa in serum K43. In the nuclear protein matrix, the antibodies to protein with mol. mass of 38 kDa stained only nucleolar periphery, while the antibodies to the protein with mol. mass of 50 kDa stained both the nucleolar periphery and all the interphase nucleus. It shows that among all components of nuclear protein matrix (lamina, internuclear network, residual nucleoli) only nucleolar periphery contains the 38 kDa protein, while the 50 kDa protein is a part of residual nucleolar periphery and takes part in nuclear protein network formation. In the interphase cells, both proteins were in situ localized in the nuclei, but one of them with mol. mass of 50 kDa was in the form of small clearly outlined granules, while the other (38 kDa) was in the form of small bright granules against the background of diffusely stained nuclei. Both proteins were also revealed as continuous ring around nucleolar periphery. During all mitotic stages, the 50 kDa protein was seen on the chromosomal periphery as a cover, and the 38 kDa protein formed separate fragments and granules around them. After nuclear and chromosome decondensation induced by hypotonic treatment, both antibodies stain interphase nuclei in diffuse manner, but in mitotic cells they stained the surface of the swollen chromosomes. The polypeptide with mol. mass of 50 kDa maintained strong connection with chromosome periphery both in norm and under condition of decondensation induced by hypotonic treatment and at subsequent recondensation in isotonic medium. In contrast, the protein with mol. mass of 38 kDa partially lost the contact with a chromosome during recondensation appearing also in the form of granules in

  13. Parentalidades Adotiva e Biológica e Suas Repercussões nas Dinâmicas Conjugais

    Directory of Open Access Journals (Sweden)

    Mariana Silva Cecílio

    Full Text Available Resumo O objetivo deste estudo foi compreender os processos de construção das parentalidades adotivas e biológicas e suas repercussões na conjugalidade. Trata-se de um estudo de caso coletivo, qualitativo e transversal. Foram entrevistados sete casais com filho(s biológico(s nascido(s antes da chegada do(s adotivo(s. Utilizou-se a técnica da história de vida e a entrevista semiestruturada, totalizando 14 entrevistas. A análise de conteúdo revelou quatro eixos temáticos. A motivação para adoção foi referida como ato tanto de amor quanto de ajuda ao próximo. Observou-se que a transição para a parentalidade, ocorrida com o nascimento do filho biológico, repercutiu de modo mais expressivo na qualidade da relação conjugal que a chegada dos demais filhos, tanto adotivos quanto biológicos. O planejamento do casal para a adoção possibilitou que os ajustes na estrutura familiar não afetassem a conjugalidade e a satisfação no relacionamento amoroso, compreendido como positivo ao longo da experiência parental.

  14. Accidentes biológicos en estudiantes de medicina de una universidad peruana: prevalencia, mecanismos y factores de riesgo

    Directory of Open Access Journals (Sweden)

    Elizabeth Inga

    2010-03-01

    Full Text Available Introducción: Una de las más serias amenazas que enfrentan los estudiantes de medicina durante su práctica clínica es la posibilidad de exposición a accidentes biológicos, debido en la mayoría de los casos a la inexperiencia y el escaso desarrollo de las habilidades manuales. Objetivos: Establecer la frecuencia, mecanismos, circunstancias y factores de riesgo de los accidentes biológicos ocurridos entre estudiantes de medicina. Diseño: Estudio descriptivo observacional de corte transversal. Institución: Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Participantes: Estudiantes de medicina. Metodología: Se aplicó una encuesta estructurada anónima, voluntaria y autoadministrada. Principales medidas de resultados: Accidentes biológicos. Resultados: En total fueron 307 entrevistados. La prevalencia de accidentes biológicos en la población estudiada fue de 51,5% (158/307. La media de accidentes biológicos en el último año fue de 1,06. El 91,1% de los estudiantes del último año presentó al menos un accidente biológico, versus 11,9% en los estudiantes del primer año. Los estudiantes del último año informaron con más frecuencia accidentes de riesgo alto para transmisión de infecciones, siendo 47,6% por pinchazo con objeto punzocortante, y 80,6% tuvo exposición a sangre; los accidentes de riesgo alto son mucho más frecuentes en quirófanos y sala de partos (51,9%. Conclusiones: La prevalencia de accidentes biológicos fue 51,5%, siendo el pinchazo la forma más frecuente. Es necesario desarrollar estrategias que permitan velar por la bioseguridad de los estudiantes de medicina.

  15. Thermodynamic analysis of biochemical systems

    International Nuclear Information System (INIS)

    Yuan, Y.; Fan, L.T.; Shieh, J.H.

    1989-01-01

    Introduction of the concepts of the availability (or exergy), datum level materials, and the dead state has been regarded as some of the most significant recent developments in classical thermodynamics. Not only the available energy balance but also the material and energy balances of a biological system may be established in reference to the datum level materials in the dead state or environment. In this paper these concepts are illustrated with two examples of fermentation and are shown to be useful in identifying sources of thermodynamic inefficiency, thereby leading naturally to the rational definition of thermodynamic efficiency of a biochemical process

  16. Biochemical structure of Calendula officinalis.

    Science.gov (United States)

    Korakhashvili, A; Kacharava, T; Kiknavelidze, N

    2007-01-01

    Calendula officinalis is a well known medicinal herb. It is common knowledge that its medicinal properties are conditioned on biologically active complex substances of Carotin (Provitamin A), Stearin, Triterpiniod, Plavonoid, Kumarin, macro and micro compound elements. Because of constant need in raw material of Calendula officinalis, features of its ontogenetic development agro-biological qualities in various eco regions of Georgia were investigated. The data of biologically active compounds, biochemical structure and the maintenance both in flowers and in others parts of plant is presented; the pharmacological activity and importance in medicine was reviewed.

  17. Enzyme and biochemical producing fungi

    DEFF Research Database (Denmark)

    Lübeck, Peter Stephensen; Lübeck, Mette; Nilsson, Lena

    2010-01-01

    We are developing a biorefinery concept for biological production of chemicals, drugs, feed and fuels using plant biomass as raw material in well-defined cell-factories. Among the important goals is the discovery of new biocatalysts for production of enzymes, biochemicals and fuels and already our...... screening of a large collection of fungal strains isolated from natural habitats have resulted in identification of strains with high production of hydrolytic enzymes and excretion of organic acids. Our research focuses on creating a fungal platform based on synthetic biology for developing new cell...

  18. Biochemical Abnormalities in Batten's Syndrome

    DEFF Research Database (Denmark)

    Clausen, Jytte Lene; Nielsen, Gunnar Gissel; Jensen, Gunde Egeskov

    1978-01-01

    The present data indicate that a group of ten patients with Batten's syndrome showed reduced activity of erythrocyte glutathione (GSH) peroxidase (Px) (glutathione: H2O2 oxidoreductase, EC 1.1.1.9.) using H2O2 as peroxide donor. Assay of erythrocyte GSHPx using H2O2, cumene hydroperoxide and t......-butyl hydroperoxide as donors also makes it possible biochemically to divide Batten's syndrome into two types: (1) one type with decreased values when H2O2 and cumene hydroperoxide are used, and (2) one type with increased values when t-butyl hydroperoxide is used. Furthermore an increased content of palmitic, oleic...

  19. Stability assessment of gas mixtures containing terpenes at nominal 5 nmol/mol contained in treated aluminum gas cylinders.

    Science.gov (United States)

    Rhoderick, George C

    2010-10-01

    Studies of climate change increasingly recognize the diverse influences exerted by terpenes in the atmosphere, including roles in particulates, ozone formation, and their oxidizing potential. Measurements of key terpenes suggest atmospheric concentrations ranging from low pmol/mol (parts per trillion) to nmol/mol (parts per billion), depending on location and compound. To accurately establish concentration trends, assess the role of terpenes in atmospheric chemistry, and relate measurement records from many laboratories and researchers, it is essential to have good calibration standards. The feasibility of preparing well-characterized, stable gas cylinder standards for terpenes at the nmol/mol level is not yet well established. Several of the world's National Metrology Institutes (NMIs) are researching the feasibility of developing primary and secondary reference gas standards at the nmol/mol level for terpenes. The US NMI, the National Institute of Standards and Technology, has prepared several nmol/mol mixtures, in treated aluminum gas cylinders, containing terpenes in dry nitrogen at nominal 5 nmol/mol for stability studies. Overall, 11 terpenes were studied for stability. An initial gas mixture containing nine terpenes, one oxygenate, and six aromatic compounds, including benzene as an internal standard, was prepared. Results for four of the nine terpenes in this initial mixture indicate stability in these treated aluminum gas cylinders for over 6 months and project long term (years) stability. Interesting results were seen for beta-pinene, which when using a linear equation rate decline predicts that it will reach a zero concentration level at day 416. At the same time, increases in alpha-pinene, D: -limonene (R-(+)-limonene), and p-cymene were observed, including camphene, a terpene not prepared in the gas mixture, indicating a chemical transformation of beta-pinene to these species. Additional mixtures containing combination of either alpha-pinene, camphor

  20. Bistability in biochemical signaling models.

    Science.gov (United States)

    Sobie, Eric A

    2011-09-20

    This Teaching Resource provides lecture notes, slides, and a student assignment for a two-part lecture on the principles underlying bistability in biochemical signaling networks, which are illustrated with examples from the literature. The lectures cover analog, or graded, versus digital, all-or-none, responses in cells, with examples from different types of biological processes requiring each. Rate-balance plots are introduced as a method for determining whether generic one-variable systems exhibit one or several stable steady states. Bifurcation diagrams are presented as a more general method for detecting the presence of bistability in biochemical signaling networks. The examples include an artificial toggle switch, the lac operon in bacteria, and the mitogen-activated protein kinase cascade in both Xenopus oocytes and mammalian cells. The second part of the lecture links the concepts of bistability more closely to the mathematical tools provided by dynamical systems analysis. The examples from the first part of the lecture are analyzed with phase-plane techniques and bifurcation analysis, using the scientific programming language MATLAB. Using these programs as a template, the assignment requires the students to implement a model from the literature and analyze the stability of this model's steady states.

  1. Biochemical abnormalities in Pearson syndrome.

    Science.gov (United States)

    Crippa, Beatrice Letizia; Leon, Eyby; Calhoun, Amy; Lowichik, Amy; Pasquali, Marzia; Longo, Nicola

    2015-03-01

    Pearson marrow-pancreas syndrome is a multisystem mitochondrial disorder characterized by bone marrow failure and pancreatic insufficiency. Children who survive the severe bone marrow dysfunction in childhood develop Kearns-Sayre syndrome later in life. Here we report on four new cases with this condition and define their biochemical abnormalities. Three out of four patients presented with failure to thrive, with most of them having normal development and head size. All patients had evidence of bone marrow involvement that spontaneously improved in three out of four patients. Unique findings in our patients were acute pancreatitis (one out of four), renal Fanconi syndrome (present in all patients, but symptomatic only in one), and an unusual organic aciduria with 3-hydroxyisobutyric aciduria in one patient. Biochemical analysis indicated low levels of plasma citrulline and arginine, despite low-normal ammonia levels. Regression analysis indicated a significant correlation between each intermediate of the urea cycle and the next, except between ornithine and citrulline. This suggested that the reaction catalyzed by ornithine transcarbamylase (that converts ornithine to citrulline) might not be very efficient in patients with Pearson syndrome. In view of low-normal ammonia levels, we hypothesize that ammonia and carbamylphosphate could be diverted from the urea cycle to the synthesis of nucleotides in patients with Pearson syndrome and possibly other mitochondrial disorders. © 2015 Wiley Periodicals, Inc.

  2. Moléculas de adesão celular: papel do microambiente tumoral na carcinogénese

    OpenAIRE

    Cardoso, Edgar António Delgado

    2009-01-01

    Trabalho final do 6º ano médico com vista à atribuição do grau de mestre (área científica de patológica) no âmbito do ciclo de estudos de Mestrado Integrado em Medicina. As moléculas de adesão celular determinam estimulação funcional, migração, ancoragem, diferenciação fenotípica e multiplicação celular. A capacidade da célula tumoral se movimentar, atravessar as paredes dos vasos e se localizar e proliferar no processo de metastização, está dependente das moléculas de adesão e da...

  3. mol2chemfig, a tool for rendering chemical structures from molfile or SMILES format to LATE X code.

    Science.gov (United States)

    Brefo-Mensah, Eric K; Palmer, Michael

    2012-10-02

    : Displaying chemical structures in LATE X documents currently requires either hand-coding of the structures using one of several LATE X packages, or the inclusion of finished graphics files produced with an external drawing program. There is currently no software tool available to render the large number of structures available in molfile or SMILES format to LATE X source code. We here present mol2chemfig, a Python program that provides this capability. Its output is written in the syntax defined by the chemfig TE X package, which allows for the flexible and concise description of chemical structures and reaction mechanisms. The program is freely available both through a web interface and for local installation on the user's computer. The code and accompanying documentation can be found at http://chimpsky.uwaterloo.ca/mol2chemfig.

  4. Influência da dinâmica transcricional no dobramento da molécula de RNA

    OpenAIRE

    Costa, Pedro Rafael [UNESP

    2016-01-01

    Uma grande variedade de sequências de RNA presentes nos transcriptomas mas com funções ainda desconhecidas tem estimulado o desenvolvimento de técnicas experimentais e computacionais que colaborem na determinação do papel dessas moléculas. Entretanto, a compreensão dos mecanismos de ação de uma dada molécula de RNA envolve não somente a determinação de sua estrutura de mínima energia livre, mas também o estudo do comportamento de suas conformações metaestáveis. Os algoritmos existentes para p...

  5. Thermal sensor properties of PANI (EB)–CSAX (X= 0⋅ 4±0⋅ 1 mol ...

    Indian Academy of Sciences (India)

    These PANI(EB)–CSA ( = 0.5, 0.4, 0.3 mol) polymer films were developed through controlled temperature atmosphere 60 ± 2°C for 60 min, and with the help of temperature dependence of resistivity (ρ) values, high temperature coefficient of resistance (TCR) i.e. values, and figure of merit (ρ ) values of these films, ...

  6. The method of loci (MoL) and memory consolidation: dreaming is not MoL-like.

    Science.gov (United States)

    Nielsen, Tore

    2013-12-01

    Certain method of loci (MoL) prerequisites--familiar, coherently ordered locations--should appear during dreaming if the latter is, in fact, elaborative memory encoding as hypothesized by Llewellyn. A review of the literature suggests that dreamed locations are neither familiar nor coherently ordered and thus unsuitable for facilitating memory in this sense. This conclusion converges with other evidence that episodic memory is dependent upon non-rapid eye movement (NREM), rather than REM, sleep.

  7. Associations between Dietary Iron and Zinc Intakes, and between Biochemical Iron and Zinc Status in Women

    Directory of Open Access Journals (Sweden)

    Karen Lim

    2015-04-01

    Full Text Available Iron and zinc are found in similar foods and absorption of both may be affected by food compounds, thus biochemical iron and zinc status may be related. This cross-sectional study aimed to: (1 describe dietary intakes and biochemical status of iron and zinc; (2 investigate associations between dietary iron and zinc intakes; and (3 investigate associations between biochemical iron and zinc status in a sample of premenopausal women aged 18–50 years who were recruited in Melbourne and Sydney, Australia. Usual dietary intakes were assessed using a 154-item food frequency questionnaire (n = 379. Iron status was assessed using serum ferritin and hemoglobin, zinc status using serum zinc (standardized to 08:00 collection, and presence of infection/inflammation using C-reactive protein (n = 326. Associations were explored using multiple regression and logistic regression. Mean (SD iron and zinc intakes were 10.5 (3.5 mg/day and 9.3 (3.8 mg/day, respectively. Median (interquartile range serum ferritin was 22 (12–38 μg/L and mean serum zinc concentrations (SD were 12.6 (1.7 μmol/L in fasting samples and 11.8 (2.0 μmol/L in nonfasting samples. For each 1 mg/day increase in dietary iron intake, zinc intake increased by 0.4 mg/day. Each 1 μmol/L increase in serum zinc corresponded to a 6% increase in serum ferritin, however women with low serum zinc concentration (AM fasting < 10.7 μmol/L; AM nonfasting < 10.1 μmol/L were not at increased risk of depleted iron stores (serum ferritin <15 μg/L; p = 0.340. Positive associations were observed between dietary iron and zinc intakes, and between iron and zinc status, however interpreting serum ferritin concentrations was not a useful proxy for estimating the likelihood of low serum zinc concentrations and women with depleted iron stores were not at increased risk of impaired zinc status in this cohort.

  8. EzMol: A Web Server Wizard for the Rapid Visualization and Image Production of Protein and Nucleic Acid Structures.

    Science.gov (United States)

    Reynolds, Christopher R; Islam, Suhail A; Sternberg, Michael J E

    2018-01-31

    EzMol is a molecular visualization Web server in the form of a software wizard, located at http://www.sbg.bio.ic.ac.uk/ezmol/. It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do not have the time or inclination to use a software molecular visualization system. EzMol allows the upload of molecular structure files in PDB format to generate a Web page including a representation of the structure that the user can manipulate. EzMol provides intuitive options for chain display, adjusting the color/transparency of residues, side chains and protein surfaces, and for adding labels to residues. The final adjusted protein image can then be downloaded as a high-resolution image. There are a range of applications for rapid protein display, including the illustration of specific areas of a protein structure and the rapid prototyping of images. Copyright © 2018. Published by Elsevier Ltd.

  9. Using a PyMOL activity to reinforce the connection between genotype and phenotype in an undergraduate genetics laboratory.

    Directory of Open Access Journals (Sweden)

    Alexandra D Simmons

    Full Text Available With the purpose of developing an activity that would help clarify genetic concepts related to the connection between genotype and phenotype and the nature of mutations, we designed a three hour teaching module using the PyMol software. The activity starts with two pre-laboratory assignments, one to learn how to use PyMol and the other to read about a specific protein or protein family. During the laboratory students are given instructions where and how to find additional information on a specific disease and its causal mutations in order to prepare a 10-minute, in-class presentation. Using a post activity, anonymous quiz, we found a statistically significant different grade distribution in students that participated in the PyMol activity relative to a control group. We also found a significant improvement in the student's comprehension when answering questions regarding the nature of mutations and protein structure. This demonstrates the utility of this simulation activity as a vehicle to improve student's understanding of specific key genetic concepts.

  10. Preparation of TiO₂ nanocrystallite powders coated with 9 mol% ZnO for cosmetic applications in sunscreens.

    Science.gov (United States)

    Ko, Horng-Huey; Chen, Hui-Ting; Yen, Feng-Ling; Lu, Wan-Chen; Kuo, Chih-Wei; Wang, Moo-Chin

    2012-01-01

    The preparation of TiO(2) nanocrystallite powders coated with and without 9 mol% ZnO has been studied for cosmetic applications in sunscreens by a co-precipitation process using TiCl(4) and Zn(NO(3))(2)·6H(2)O as starting materials. XRD results show that the phases of anatase TiO(2) and rutile TiO(2) coexist for precursor powders without added ZnO (T-0Z) and calcined at 523 to 973 K for 2 h. When the T-0Z precursor powders are calcined at 1273 K for 2 h, only the rutile TiO(2) appears. In addition, when the TiO(2) precursor powders contain 9 mol% ZnO (T-9Z) are calcined at 873 to 973 K for 2 h, the crystallized samples are composed of the major phase of rutile TiO(2) and the minor phases of anatase TiO(2) and Zn(2)Ti(3)O(8). The analyses of UV/VIS/NIR spectra reveal that the absorption of the T-9Z precursor powders after being calcined has a red-shift effect in the UV range with increasing calcination temperature. Therefore, the TiO(2) nanocrystallite powders coated with 9 mol% ZnO can be used as the attenuate agent in the UV-A region for cosmetic applications in sunscreens.

  11. Efectos de la irradiación iónica en hielos de moléculas carbonadas

    Science.gov (United States)

    Satorre, M. A.

    En Astrofísica podemos encontrar numerosos contextos en los cuales se observan moléculas en estado sólido que, en condiciones estándar de presión y temperatura, se encontrarían como gases o líquidos. Dichas moléculas se denominan hielos y han sido observadas en nubes densas del medio interestelar, en envolturas circumestelares, en satélites del Sistema Solar, en cometas, etc. Los hielos pueden ser alterados en su composición química debido a diversos factores como por ejemplo variaciones de temperatura o aportes energéticos por parte de la irradiación, ya sea tanto de fotones ultravioleta como de iones. Dependiendo del escenario astrofísico que analicemos, unos factores cobran más importancia que otros. Los experimentos de laboratorio muestran el efecto que produce sobre la composición de los hielos la irradiación iónica, en particular sobre los que contenían alguna molécula con átomos de carbono. Dicha composición se analiza con espectroscopía IR en el rango de 2 a ˜ 25μ m. La aplicabilidad de los resultados de los experimentos es distinta dependiendo de la composición química inicial de los hielos, del tipo de ion utilizado y de la dosis total de irradiación. Existen efectos generales de la irradiación sobre la materia en los experimentos de relevancia astrofísica como son: - la formación de nuevas moléculas, que pueden incluir o no el ion incidente; - la progresiva pérdida de hidrógeno (carbonización) cuando irradiamos muestras que originalmente contienen una determinada relación carbono/hidrógeno; - la variación de la temperatura de sublimación que presentan algunos hielos. Esto puede suceder tanto en hielos que estaban presentes antes de la irradiación como en hielos formados por ésta. Se presentará el papel del ion en la formación de nuevas moléculas a partir de las que originalmente se encontraban en el hielo. Al penetrar en él, el ion provoca distintos procesos como rotura de enlaces y excitaciones electr

  12. Mesofauna edáfica: indicador biológico de la calidad del suelo

    Directory of Open Access Journals (Sweden)

    Ana Socarrás

    Full Text Available La búsqueda, el uso y la aplicación de los indicadores de la calidad del suelo se han intensificado en las últimas décadas, debido a la necesidad de preservar este recurso no renovable e indispensable para la vida humana, ante el deterioro creciente. La selección de los indicadores se ha dirigido, fundamentalmente, a conocer el efecto de los usos aplicados y el curso de la rehabilitación de los suelos degradados o contaminados. Los grupos que integran la mesofauna edáfica son sensibles a las perturbaciones naturales y antrópicas del medio, las que provocan cambios en su composición específica y su abundancia, y ocasionan la pérdida de especies y de su diversidad, con la consiguiente disminución de la estabilidad y la fertilidad del suelo. Por ello la mesofauna edáfica es considerada como un buen indicador biológico de su estado de conservación. El número, la densidad y el balance de sus grupos permiten predecir y evaluar las transformaciones ocasionadas por la aplicación de diferentes métodos de producción agrícola en condiciones edafoclimáticas específicas, así como considerar integralmente el funcionamiento del ecosistema.

  13. O ensino e a saúde: um olhar biológico

    Directory of Open Access Journals (Sweden)

    Nelson Vaz

    1999-01-01

    Full Text Available Os seres vivos são sistemas determinados por suas estruturas. O ensino é impossível, mas a aprendizagem é inevitável, um comentário de um observador sobre algum aspecto das mudanças constantes que ocorrem durante o viver. Professor é aquele que abre um espaço de convivência. Não existem interações instrutivas na natureza. A saúde e a biologia dos seres vivos são fenômenos estudados em domínios distintos, a primeira sendo definida pela cultura. Como um fenômeno biológico, a saúde ou a doença são configurações relacionais do organismo com seu meio e, como tais, são fenômenos descritos no domínio das interações do organismo. Por esse entendimento, a saúde individual é um fenômeno social.

  14. Investigación biológica y psicopatología descriptiva

    Directory of Open Access Journals (Sweden)

    Germán Berríos

    1983-06-01

    Full Text Available El autor hace un análisis de la psicopatología descriptiva moderna, de la contribución que ha hecho el establecimiento de tipologías biológicas y de sus presupuestos epistemológicos. Revisa las consecuencias, que a nivel de ciertas clasificaciones nosográficas e instrumentos tales como el DSM-1, el DSM-11, el PSE, etc., ha tenido la búsqueda de confiabilidad yvalidez. Asimismo, señala el peligro del fenómeno de "cierre" y las alternativas para enfrentarlo.Plantea también la noción de unidad de análisis y la importancia de adecuarla a modelos de investigación funcional que permitan un abordajemultidimensional del fenómeno psicopatológico.Esto último permitiría clasificaciones con una mayor probabilidad de tener representación orgánica. Finalmente, el autor señala la legitimidad del estudio profundo del caso individual.

  15. Cloning and Biochemical Characterization of HIV Integrase

    Science.gov (United States)

    1989-12-15

    biochemical assays for the interaction of the HIV Intebration protein, IN, with a specific DNA target, the viral LTRs. The gene encoding IN has been subcloned ...FILE COPY CO CONTRACT NO.: DAMD7-88-C-8126 r TITLE: CLONING AND BIOCHEMICAL CHARACTERIZATION OF HIV INTEGRASE I- SPRINCIPAL INVESTIGATOR: ELLEN...ELEMENT No. NO. 392- NO. IACCESSION NO. 1 . TITLE (indude Security Classification)630A I315D49C08 (U) Cloning and Biochemical Characterization of the HIV

  16. Biochemical adaptation of phytopathogenic fungi, Sclerotium rolfsii ...

    African Journals Online (AJOL)

    Biochemical adaptation of phytopathogenic fungi, Sclerotium rolfsii, in response to temperature stress. Natthiya Buensanteai, Kanjana Thumanu, Khanistha Kooboran, Dusit Athinuwat, Sutruedee Prathuangwong ...

  17. Biochemical Markers in Neurocritical Care

    Directory of Open Access Journals (Sweden)

    Omidvar Rezae

    2016-07-01

    Full Text Available During the past two decades, a variety of serum or cerebrospinal fluid (CSF biochemical markers in daily clinical practice have been recommended to diagnose and monitor diverse diseases or pathologic situations. It will be essential to develop a panel of biomarkers, to be suitable for evaluation of treatment efficacy, representing distinct phases of injury and recovery and consider the temporal profile of those. Among the possible and different biochemical markers, S100b appeared to fulfill many of optimized criteria of an ideal marker. S100b, a cytosolic low molecular weight dimeric calciumbinding protein from chromosome 21, synthesized in glial cells throughout the CNS, an homodimeric diffusible, belongs to a family of closely related protein, predominantly expressed by astrocytes and Schwann cells and a classic immunohistochemical marker for these cells, is implicated in brain development and neurophysiology. Of the 3 isoforms of S-100, the BB subunit (S100B is present in high concentrations in central and peripheral glial and Schwann cells, Langerhans and anterior pituitary cells, fat, muscle, and bone marrow tissues. The biomarker has shown to be a sensitive marker of clinical and subclinical cerebral damage, such as stroke, traumatic brain injury, and spinal cord injury. Increasing evidence suggests that the biomarker plays a double function as an intracellular regulator and an extracellular signal of the CNS. S100b is found in the cytoplasm in a soluble form and also is associated with intracellular membranes, centrosomes, microtubules, and type III intermediate filaments. Their genomic organization now is known, and many of their target proteins have been identified, although the mechanisms of regulating S100b secretion are not completely understood and appear to be related to many factors, such as the proinflammatory cytokines, tumor necrosis factor alpha (TNF-a, interleukin (IL-1b, and metabolic stress. 

  18. Biochemical characterization of the GM2 gangliosidosis B1 variant

    Directory of Open Access Journals (Sweden)

    Tutor J.C.

    2004-01-01

    Full Text Available The deficiency of the A isoenzyme of ß-hexosaminidase (Hex produced by different mutations of the gene that codes for the alpha subunit (Tay-Sachs disease has two variants with enzymological differences: the B variant consists of the absence of Hex A isoenzyme and the B1 variant produces an inactive Hex A isoenzyme for the hydrolysis of the GM2 ganglioside and synthetic substrates with negative charge. In contrast to the early childhood form of the B variant, the B1 variant appears at a later clinical stage (3 to 7 years of age with neurodegenerative symptoms leading to the death of the patient in the second decade of life. The most frequent mutation responsible for the GM2 gangliosidosis B1 variant is R178H, which has a widespread geographic and ethnic distribution. The highest incidence has been described in Portugal, which has been suggested as the point of origin of this mutation. Biochemical characterization of this lysosomal disease is carried out using negatively charged synthetic alpha subunit-specific sulfated substrates, since Hex A isoenzyme heat-inactivation assays are not applicable. However, the determination of the apparent activation energy of Hex using the neutral substrate 3,3'-dichlorophenolsulfonphthaleinyl N-acetyl-ß-D-glucosaminide, may offer a valid alternative. The presence of an alpha subunit in the alphaß heterodimer Hex A means that its activation energy (41.8 kJ/mol is significantly lower than that of the ßß homodimer Hex B (75.1 kJ/mol; however, as mutation inactivates the alpha subunit, the Hex A of the B1 variant presents an activation energy that is similar to that of the Hex B isoenzyme.

  19. Espacio biológico: Parte I: La inserción diente-encía

    OpenAIRE

    A. Delgado Pichel; P. Inarejos Montesinos; M. Herrero Climent

    2001-01-01

    Se denomina espacio biológico a la unión dentogingival, que está constituida por el epitelio de unión y el tejido conectivo de inserción de la encía. Cuando se habla de espacio biológico no sólo se debe pensar en la longitud de la inserción gingival, sino que se debe relacionar con el grosor de la encía, el biotipo periodontal y la profundidad del surco gingival, puesto que todos estos parámetros se integran, y deben ser tenidos en cuenta para comprender de manera exacta la morfología del tej...

  20. Aplicação de técnicas ópticas em amostras biológicas

    OpenAIRE

    Camilla Karla Brites Queiroz M de Oliveira

    2008-01-01

    No presente trabalho, utilizamos duas técnicas ópticas para caracterização in vivo de amostras biológicas: a Microscopia de Desfocalização e o Espalhamento de Luz. As amostras biológicas escolhidas foram os macrófagos, que são células do sistema imune inato e as hemácias, as células vermelhas do sangue, que entre outras funções é responsável pelo transporte de oxigênio e gás carbônico no organismo. A Microscopia de Desfocalização, desenvolvida em nosso laboratório em 2003 e recentemente revis...

  1. Informatividade dos lucros contábeis e divulgação dos ativos biológicos: evidências brasileiras

    OpenAIRE

    Holanda, Allan Pinheiro

    2013-01-01

    A pesquisa tem o objetivo de investigar a influência exercida pela divulgação dos ativos biológicos na informatividade dos lucros contábeis nas firmas brasileiras listadas na BM&FBovespa, considerando-se o período de 2010 a 2011. Para a investigação da informatividade dos ativos biológicos foi utilizada a adaptação dos modelos propostos por Easton e Harris (1991) e Francis e Schipper (1999). Os ativos biológicos, por sua vez, foram mensurados de duas maneiras, pela simples existência da c...

  2. Corredores biológicos: una estrategia de conservación en el manejo de cuencas hidrográficas

    OpenAIRE

    Feoli-Boraschi, Sergio; Compañia Nacional de Fuerza y Luz

    2012-01-01

    Se analiza la importancia de los corredores biológicos para el manejo de los recursos naturales, específicamente en el manejo de cuencas. Se define corredor biológico como un espacio geográfico donde se da la posibilidad de hacer manejo sostenible y las personas puedan actuar de manera que no haya efectos negativos contra la flora y la fauna del lugar. Se toma como ejemplo el corredor biológico “Paso de las Nubes”, que pretende unir a través de bosque fragmentados la Zona Protectora “Alberto ...

  3. First application of MOL {sup registered} CLEAN - process in a cooling tower of an oil refinery; Erstmalige Anwendung des MOL {sup registered} CLEAN - Verfahrens in einem Kuehlkreislauf einer Erdoelraffinerie

    Energy Technology Data Exchange (ETDEWEB)

    Ernhofer, R.; Karl, G.; Wentzlau, F. [BAYERNOIL Raffineriegesellschaft mbH, Ingolstadt (Germany); Koppe, J.; Lausch, H. [MOL Katalysatortechnik GmbH, Merseburg (Germany)

    2005-10-01

    Biological loads of cooling cycles in oil refineries can cause efficiency losses. The key components of biological loads are bio layers. With the help of the MOL {sup registered} CLEAN process, these bio layers are sustainably eliminated. This technique was installed in a cooling tower battery of the BAYERNOIL Raffineriegesellschaft mbH in Neustadt on the Danube river in October 2004. After installation, all bio layers were eliminated from the sprinkled packages of cooling tower and from the heat exchangers surfaces without any unwelcome side effects. In this way, the performance of the cooling cycle was enhanced with positive consequences on the global refinery efficiency. (orig.)

  4. Investigador Invitado. Control Biológico en Agroecosistemas Mediante el Manejo de Insectos Entomófagos

    Directory of Open Access Journals (Sweden)

    Nicholls Clara Inés

    1998-06-01

    Full Text Available La importancia que tienen los enemigos naturales de los organismos nocivos ha sido reconocida desde hace varias décadas. Desafortunadamente la implantación del control biológico no ha tenido la dimensión deseada. El empleo indiscriminado de productos biocidas ha alterado la biodiversidad de los agroecosistemas. Los parasitoides y predatores han sufrido los efectos nocivos de los plaguicidas. Los enemigos naturales de las plagas juegan un papel trascendental en la regulación las poblaciones de insectos nocivos. Los predatores de la clase insecta se registran en diversos órdenes y la abundancia de especies es impresionante. Pero el conocimiento que se tiene sobre su importancia aún es parcial. Pero el conocimiento que se tiene sobre su importancia aun es parcial. En muchos países no se ha precisado aun la bondad de estos organismos y se les protege. En el caso de los parasitoides ocurre algo similar. Se afirma que su diversidad biótica es incalculable, pero se explotan muy pocas especies. En estos dos grupos descansan los proyectos de Control Biológico clásico. Los éxitos en proyectos de control biológico se reconocen y se han expandido en varios países pero se requiere de un mayor impulso. Debido a las exigencias de una producción agrícola sostenible se debe de apoyar en Control Biológico de Plagas. En este documento se plantean aspectos generales sobre el tema.

  5. Poliquetos (Annelida: Polychaeta como indicadores biológicos de contaminación marina: casos en Colombia

    Directory of Open Access Journals (Sweden)

    Vanessa Fernández Rodríguez

    2015-06-01

    Full Text Available El concepto de Indicador Biológico ha sido usado sin mayor precaución al momento de emplear ciertos organismos en programas de monitoreo ambiental, lo cual genera confusión. Uno de los objetivos de este trabajo fue resaltar la importancia en la consolidación de una definición precisa de este concepto a partir de información disponible, proponiendo una definición para el mismo. Las características ecológicas de los poliquetos permiten que, al estar en contacto permanente con diferentes tipos de contaminantes, respondan bioacumulando, disminuyendo o aumentando su abundancia, según sea la especie, hecho que posiciona este tipo de organismos como potenciales indicadores de contaminación marina. En este artículo se presenta de manera concreta un análisis de la literatura disponible para poliquetos en el campo de los indicadores biológicos, resaltando cómo éstos han sido usados en diferentes metodologías, con ejemplos a internacionales, así como una selección especial para Colombia. De los resultados más sobresalientes se encontró que Capitella capitata es la especie más estudiada al estar asociada con ambientes contaminados a causa del incremento de materia orgánica y es la única especie reportada en el país como indicador biológico usando las técnicas clásicas de bioindicación. Finalmente, se reitera la importancia de iniciar investigaciones sobre los aspectos ecológicos, ecotoxicológicos y bioensayos de laboratorio con otras especies de poliquetos para validar cuáles especies y por qué pueden ser consideradas como indicadores biológicos para el país.

  6. Fatores associados à exposição ocupacional com material biológico entre profissionais de enfermagem

    Directory of Open Access Journals (Sweden)

    Nádia Bruna da Silva Negrinho

    Full Text Available RESUMO Objetivo: identificar os fatores associados à exposição ocupacional com material biológico entre profissionais de enfermagem. Método: estudo transversal, realizado em um hospital, de alta complexidade, do interior do Estado de São Paulo, Brasil. Foram entrevistados profissionais de enfermagem no período de março a novembro de 2015. Todos os aspectos éticos foram contemplados. Resultado: dentre 226 profissionais entrevistados, 17,3% sofreram exposição ocupacional com material biológico potencialmente contaminado, sendo 61,5% por via percutânea. Fatores, como a faixa etária (p=0,003, a experiência na enfermagem (p=0,015 e na instituição, (p=0,032 estiveram associados ao acidente. Conclusão: a maior parte dos acidentes com material biológico entre os profissionais de enfermagem ocorreu por via percutânea sendo que a idade, experiência profissional e na instituição compreenderam fatores associados à exposição ocupacional.

  7. Control biológico del entrenamiento de resistencia. Biological control of endurance training.

    Directory of Open Access Journals (Sweden)

    González Gross, Marcela

    2006-01-01

    Full Text Available ResumenLa alta exigencia en los deportistas de elite hace cada vez más necesario controlar el proceso de adaptación al entrenamiento. El objetivo de esta revisión es analizar la información biológica de un análisis de sangre, al objeto de obtener información de la carga de entrenamiento en atletas de resistencia. La mayor parte de los parámetros sanguíneos han sido empleados, más que para determinar el proceso del entrenamiento, precisamente, para lo opuesto: el sobreentrenamiento. La concentración en plasma de sustratos metabólicos (glucosa y ácidos grasos no son parámetros que pueda utilizarse para controlar el entrenamiento, debido a las bajas especificidad y sensibilidad. No obstante, la concentración de determinados enzimas que intervienen en la utilización de los sustratos puede ser importante. Valores de creatín kinasa superiores a 200 U/l en una persona sana sugiere claramente que la carga de entrenamiento total de una determinada sesión ha sido elevada. La concentración en plasma de algún producto de degradación del catabolismo también puede señalar la adaptación del organismo al entrenamiento. La concentración de ácido láctico en plasma es la herramienta más común en la valoración de la carga de entrenamiento. La concentración de urea es un buen marcador biológico de la carga de entrenamiento. Valores superiores a 8 mmol/l en varones y de 6,5 mmol/l en mujeres, indican que el entrenamiento ha sido muy intenso. La determinación de otros productos (amonio o sustratos (glutamina se han utilizado para detectar el sobreentrenamiento.AbstractThe high exigency in the elite sportsmen does more necessary to control the process of training adaptation. The purpose of this review is to analyze the biological information of a blood analysis to obtain data of load training in endurance athletes. Most blood parameters has been used to evaluate the overtraining state instead of determining the training process. The

  8. Control biológico in vitro de enfermedades fungosas en tomate Lycopersicum esculentum Mill

    Directory of Open Access Journals (Sweden)

    A. C. Michel-Aceves

    2008-01-01

    Full Text Available El objetivo fue obtener e identificar aislados nativos de Trichoderma spp. en suelos sembrados con tomate en Tlayacapan, Morelos (México, con problemas de Alternaria solani y Phytophthora infestans; asimismo, determinar su capacidad antagónica in vitro. Trichoderma se aisló directamente del suelo por el método de dilución en placa con medio de cultivo papa-dextrosa-agar (PDA. La capacidad antagónica se evaluó mediante el método del papel celofán y la clase de antagonismo con la técnica de cultivos duales. Los datos se sometieron a un análisis de varianza y pruebas de Tukey. Se obtuvieron 20 aislados de Trichoderma. El rango de porcentajes de inhibición del crecimiento micelial de los fitopatógenos por los aislados varió desde 38.8 a 81.3% en A. solani; y desde 16.3 a 85.5% en P. infestans. Se seleccio-naron 10 aislados que inhibieron al menos el 65% del crecimiento, que pertenecen a las especies: T. harzianum (Thz, T. longibrachiatum (Tl y T. koningii (Tk. Los aislados Tl-17, Tl-19, Tl- 20, Tl-21, Thz-16 y Tk-4, seleccionados contra A. solani, presentan antagonismo clase 1, sobrecreciendo al fitopatógeno y esporulando sobre él. Los aislados THz-17, Thz-18, Tl-17, Tl-18 y Tl-19 seleccionados contra P. infestans, presentan antagonismo clase 2 y Thz19 antagonismo clase 3. Por su acción antagónica in vitro sobre P. infestans y A. solani, puede considerarse a Trichoderma como agente promisorio en el control biológico de las enfermedades que ocasionan estos fitopatógenos.

  9. Control biológico en el cultivo del arroz en Cuba. (Resultados 1970 – 2005.

    Directory of Open Access Journals (Sweden)

    Rafael Meneses

    2007-12-01

    Full Text Available El cultivo del arroz en Cuba está afectado por diversas plagas, fundamentalmente: Tagosodes orizicolus, Lissorhoptrus brevirostris, Oebalus insularis y Spodoptera frugiperda. A partir de 1970 se iniciaron los estudios sobre los principales controles biológicos de estas plagas. Para T. orizicolus se ha determinado que Paranagrus perforator y Tytthus parviceps son los principales enemigos naturales de esta plaga. El porcentaje de huevos de T. orizicolus parasitados por P. perforator ha llegado al 50.7% en la etapa de germinación a ahijamiento activo de la planta de arroz. Además, a esta plaga se le ha detectado control por los parasitoides Elenchus sp. y Gonatopus sp. L. brevirostris ha sido manejado en los arrozales con los hongos Metarhizium anisopliae y Beauveria bassiana, obteniendo muy buenos resultados, tanto en el control como en las afectaciones ocasionadas por este insecto. La aplicación de M. anisopliae cepa Niña Bonita resulta satisfactorio para el control de O. insularis. Se ha determinado diversos depredadores, parasitoides y hongos que en determinadas épocas del año ejercer buen control de S. frugiperda, destacándose dentro de ellos: Coleomegilla cubensis, Chelonus texanus y Telenomus spp. C. texanus ha presentado un nivel de parasitismo superior al 80.0 % y Telenomus sp. de 93 %, en condiciones de producción arrocera. Con la presencia de arañas en los arrozales se logra disminuir considerablemente las poblaciones de insectos plagas.

  10. Estudos cristalográficos em macromoléculas biológicas: Aplicações em calgranulina C de granulócitos porcinos, tripanotiona redutase deTrypanosoma cruzi e fosfolipase A2 extraída do veneno da serpente Bothrops moojeni.

    OpenAIRE

    Maria Cristina Nonato Costa

    1997-01-01

    A cristalografia de raios-X e um método de singular importância para a determinação da estrutura de macromoléculas. A importância em resolver estruturas de proteínas continua a crescer em campos variando desde a bioquímica e biofísica básicas ate o desenvolvimento farmacêutico e biotecnologia. o presente trabalho esta relacionado com os estudos cristalográficos de três diferentes moléculas biológicas. Calgranulina C de granulócitos porcinos, uma proteína que liga cálcio, supostamente envolvid...

  11. Diagnósticos de enfermagem de pacientes internados em enfermaria de Moléstias Infecciosas

    OpenAIRE

    Neves,Jamile Fujishima; Paixão,Fábio Ricardo Consorti; Lima,Tiago Cristiano de; Ceolim,Maria Filomena

    2010-01-01

    OBJETIVOS: Identificar os diagnósticos de enfermagem de pacientes internados na Unidade de Moléstias Infecciosas e Parasitárias (MI) de um hospital universitário; e analisar título, fator relacionado e características definidoras dos diagnósticos e sua adequação à Taxonomia NANDA 2008. MÉTODOS: Estudo descritivo, transversal e retrospectivo abrangendo 50 prontuários de pacientes internados em julho de 2008. Os diagnósticos foram comparados aos propostos pela Taxonomia NANDA 2008. RESULTADOS: ...

  12. NMR study on the Li diffusion in a cathode material of amorphous vanadium pentoxide-5 mol% phosphorus pentoxide

    International Nuclear Information System (INIS)

    Asai, T.; Sugimoto, S.; Kawai, S.

    1989-01-01

    Diffusion properties of Li ion in a cathode material of amorphous Li chi V 2 O 5 with 5 mol% P 2 O 5 (chi=0.2-2) studied by means of Li NMR. From the relaxation time, the diffusion coefficient at 25 0 C is obtained. From the second moment, Li + ions seemed to occupy sites approximately 2.9 A apart in a large cavity similar to that in the crystalline V 2 O 5 . It is suggested that there are three kinds of sites for the Li + ion in the cavity, and that the ion changes the site of one kind to the others at chi≅0.6

  13. Caracterización de alteraciones en la molécula de hemoglobina en afrodescendientes colombianos

    OpenAIRE

    Castillo, Martha; Oliveros, Ana Lucia

    2014-01-01

    Objetivo: caracterizar alteraciones de la molécula de hemoglobina en afrodescendiente entre 18 a 50 años, a través de hemograma, estudio de sangre periférico, proteína C Reactiva, ferritina sérica, receptor soluble de transferrina sRTf y electroforesis de Hemoglobina. Materiales y métodos: Esta investigación contó con un total de 56 muestras:10 hombres y 46 mujeres.Resultados: La población en estudio perteneciente al distrito de Aguablanca en la Ciudad de Cali presentó alteraciones en la cade...

  14. PENGARUH RASIO MOL REAKTAN DAN LAMA SULFONASI TERHADAP KARAKTERISTIK METHYL ESTER SULFONIC (MES DARI METIL ESTER MINYAK SAWIT

    Directory of Open Access Journals (Sweden)

    Sri Hidayati

    2016-04-01

    Full Text Available An experiment of sulfonation process of methyl ester to produce methyl ester sulfonates (MES was caried out using methyl ester palm oil in factorial design and NaHSO as sulfonating agent with variation of ratio mol NaHSO : methyl ester (1:1.25, 1:1.5, 1:1.75 and 1:2 and sulfonation time (3 hour (L1, 4.5 hour (L2 and 6 hour (L3. The result showed that the best sulfonation condition present in 1:1,5 mol ratio and sulfonation time of 4,5 hour. The best characteristic of MES was produced emulsion stability of 68.25%, acid value of 2.57 mg KOH/g, iod value 10.91 g 1od/100 g sample, interfacial tension of 1.806 dyne/cm at MES concentration of 1% (w/w. The optimal salinity occured at concentration of 20.000 ppm which 1FT value of 0.0055 dyne/cm. Heating at a temperature of 800 C for 30 days with the addition of 2 3. Keywords: MES, sulfonation, NaHSO   ABSTRAK Sebuah penelitian tentang proses produksi metil ester sulfonat menggunakan bahan baku metil ester minyak kelapa sawit dilakukan secara faktorial menggunakan NaHSO sebagai agen pensulfonasi dengan variasi rasio mol NaHSO 1:1,25, 1:1,5, 1:1,75 dan 1:2 dan lama sulfonasi.dengan variasi 3; 45; dan 6 jam. Hasil penelitian menunjukkan bahwa kondisi proses sulfonasi terbaik terdapat pada rasio metil ester dan mol reaktan 1:1,5 dan  lama reaksi  4,5 jam dan suhu reaksi lOOoC yang menghasilkan nilai stabilitas emulsi 68,25%, bilangan asam 2,57 mg KOH/g sampel, bilangan iod 10,91 g 1od/100 g sampel. Konsentrasi metil ester sulfonat MES terbaik untuk menghasilkan 1FT terendah adalah 1% (b/b yaitu 1,806 dyne/cm, salinitas optimal terjadi pada 20.000 ppm NaCl dengan nilai 1FT 0,0055 dyne/cm. 0,098 dyne/cm. Kata kunci: MES, proses sulfonasi, NaHSO

  15. Biochemical characterization of the maltokinase from Mycobacterium bovis BCG

    Directory of Open Access Journals (Sweden)

    Lamosa Pedro

    2010-05-01

    Full Text Available Abstract Background Maltose-1-phosphate was detected in Mycobacterium bovis BCG extracts in the 1960's but a maltose-1-phosphate synthetase (maltokinase, Mak was only much later purified from Actinoplanes missouriensis, allowing the identification of the mak gene. Recently, this metabolite was proposed to be the intermediate in a pathway linking trehalose with the synthesis of glycogen in M. smegmatis. Although the M. tuberculosis H37Rv mak gene (Rv0127 was considered essential for growth, no mycobacterial Mak has, to date, been characterized. Results The sequence of the Mak from M. bovis BCG was identical to that from M. tuberculosis strains (99-100% amino acid identity. The enzyme was dependent on maltose and ATP, although GTP and UTP could be used to produce maltose-1-phosphate, which we identified by TLC and characterized by NMR. The Km for maltose was 2.52 ± 0.40 mM and 0.74 ± 0.12 mM for ATP; the Vmax was 21.05 ± 0.89 μmol/min.mg-1. Divalent cations were required for activity and Mg2+ was the best activator. The enzyme was a monomer in solution, had maximal activity at 60°C, between pH 7 and 9 (at 37°C and was unstable on ice and upon freeze/thawing. The addition of 50 mM NaCl markedly enhanced Mak stability. Conclusions The unknown role of maltokinases in mycobacterial metabolism and the lack of biochemical data led us to express the mak gene from M. bovis BCG for biochemical characterization. This is the first mycobacterial Mak to be characterized and its properties represent essential knowledge towards deeper understanding of mycobacterial physiology. Since Mak may be a potential drug target in M. tuberculosis, its high-level production and purification in bioactive form provide important tools for further functional and structural studies.

  16. immunological arthritis Prevalence of biochemical and abnormalities ...

    African Journals Online (AJOL)

    1991-02-02

    Feb 2, 1991 ... Tile prevalence of biochemical and immunological abnormali- ties was studied in a group of 256 patients with rheumatoid arthritis (104 coloureds, 100 whites and 52 blacks). The most common biochemical abnormalities detected were a reduction in the serum creatinine value (43,4%), raised globulins (39 ...

  17. Prevalence of biochemical and immunological abnormalities in ...

    African Journals Online (AJOL)

    Tile prevalence of biochemical and immunological abnormalities was studied in a group of 256 patients with rheumatoid arthritis (104 coloureds, 100 whites and 52 blacks). The most common biochemical abnormalities detected were a reduction in the serum creatinine value (43,4%), raised globulins (39,7%), raised serum ...

  18. Evaluation of Haematological and Biochemical Parameters of ...

    African Journals Online (AJOL)

    After 84 days of exposure, blood was collected and used in conducting haematological and biochemical analyses. Exposure of water to crude oil caused increased levels in chloride, conductivity, salinity, magnesium, biochemical oxygen demand, chemical oxygen demand, turbidity and Nitrate. The crude oil contaminated ...

  19. Serum Biochemical Phenotypes in the Domestic Dog.

    Directory of Open Access Journals (Sweden)

    Yu-Mei Chang

    Full Text Available The serum or plasma biochemical profile is essential in the diagnosis and monitoring of systemic disease in veterinary medicine, but current reference intervals typically take no account of breed-specific differences. Breed-specific hematological phenotypes have been documented in the domestic dog, but little has been published on serum biochemical phenotypes in this species. Serum biochemical profiles of dogs in which all measurements fell within the existing reference intervals were retrieved from a large veterinary database. Serum biochemical profiles from 3045 dogs were retrieved, of which 1495 had an accompanying normal glucose concentration. Sixty pure breeds plus a mixed breed control group were represented by at least 10 individuals. All analytes, except for sodium, chloride and glucose, showed variation with age. Total protein, globulin, potassium, chloride, creatinine, cholesterol, total bilirubin, ALT, CK, amylase, and lipase varied between sexes. Neutering status significantly impacted all analytes except albumin, sodium, calcium, urea, and glucose. Principal component analysis of serum biochemical data revealed 36 pure breeds with distinctive phenotypes. Furthermore, comparative analysis identified 23 breeds with significant differences from the mixed breed group in all biochemical analytes except urea and glucose. Eighteen breeds were identified by both principal component and comparative analysis. Tentative reference intervals were generated for breeds with a distinctive phenotype identified by comparative analysis and represented by at least 120 individuals. This is the first large-scale analysis of breed-specific serum biochemical phenotypes in the domestic dog and highlights potential genetic components of biochemical traits in this species.

  20. Diversity in Biochemical Characteristics and Antibiotics Resistant ...

    African Journals Online (AJOL)

    In this study, biochemical and antibiotic susceptibility tests were carried out on one hundred and fifty poultry isolates of Escherichia coli using Microscan® Dried Gram-negative Breakpoint Combo Pannels. The microscan panel analysed bacterial isolates for 24 biochemical tests and 23 to 25 antimicrobial agents following ...

  1. Classification of a Haemophilus influenzae ABC Transporter HI1470/71 through Its Cognate Molybdate Periplasmic Binding Protein, MolA

    Energy Technology Data Exchange (ETDEWEB)

    Tirado-Lee, Leidamarie; Lee, Allen; Rees, Douglas C.; Pinkett, Heather W. (CIT); (NWU)

    2014-10-02

    molA (HI1472) from H. influenzae encodes a periplasmic binding protein (PBP) that delivers substrate to the ABC transporter MolB{sub 2}C{sub 2} (formerly HI1470/71). The structures of MolA with molybdate and tungstate in the binding pocket were solved to 1.6 and 1.7 {angstrom} resolution, respectively. The MolA-binding protein binds molybdate and tungstate, but not other oxyanions such as sulfate and phosphate, making it the first class III molybdate-binding protein structurally solved. The {approx}100 {mu}M binding affinity for tungstate and molybdate is significantly lower than observed for the class II ModA molybdate-binding proteins that have nanomolar to low micromolar affinity for molybdate. The presence of two molybdate loci in H. influenzae suggests multiple transport systems for one substrate, with molABC constituting a low-affinity molybdate locus.

  2. Zooplancton de três piscinas biológicas em que as macrófitas apresentam diferentes graus de desenvolvimento

    OpenAIRE

    Geraldes, Ana Maria

    2008-01-01

    As piscinas biológicas são pequenos lagos construídos onde são recriadas as condições ecológicas que ocorrem em ecossistemas lacustres naturais. Ao contrário das piscinas convencionais a depuração da água é realizada por filtros biológicos de macrófitas.

  3. Organic and biochemical synthesis group

    International Nuclear Information System (INIS)

    Anon.

    1976-01-01

    Stable isotopes, because of their unique properties and non-radioactive nature, have great potential for many fields of science and technology. In particular, isotopes of carbon, nitrogen, oxygen, and sulfur (the basic building blocks of all biological molecules) would be widely used in biomedical and environmental research if they were economically available in sufficient quantities and in the required chemical forms. The major objective of our program continues to be stimulation of the widespread utilization of stable isotopes and commercial involvement through development and demonstration of applications which have potential requirements for large quantities of isotopes. Thus, demand will be created which is necessary for large-scale production of stable isotopes and labeled compounds and concomitant low unit costs. The program continues to produce a variety of labeled materials needed for clinical, biomedical, chemical, and environmental applications which serve as effective demonstrations of unique and advantageous utilization of stable isotopes. Future commercial involvement should benefit, and is a consideration in our research and development, from the technology transfer that can readily be made as a result of our organic and biochemical syntheses and also of various techniques involved in applications

  4. BEAMDB and MolD - databases for atomic and molecular collisional and radiative processes: Belgrade nodes of VAMDC

    Science.gov (United States)

    Marinković, Bratislav P.; Jevremović, Darko; Srećković, Vladimir A.; Vujčić, Veljko; Ignjatović, Ljubinko M.; Dimitrijević, Milan S.; Mason, Nigel J.

    2017-06-01

    We present two atomic and molecular (A&M) databases, MolD and BEAMDB, hosted by the SerVO - the Serbian virtual observatory (http://servo.aob.rs). These databases and web applications have been implemented in accordance to the standards developed by Virtual Atomic and Molecular Data Centre (VAMDC, http://www.vamdc.eu). The MolD database contains photo-dissociation cross-sections for individual rovibrational states of the diatomic molecular ions and rate coefficients for the atom-Rydberg atom chemi-ionisation and inverse electron-ion-atom chemi-recombination processes. The Belgrade electron/atom(molecule) database (BEAMDB) provides collisional data for electron interactions with atoms and molecules. Differential cross sections (DCS) are presented for both elastic and inelastic (excitation) cross sections in tabulated data tables. These DCS data are integrated over a full range of scattering angles in order to achieve integral, momentum transfer and viscosity cross sections as functions of impact electron energy. Beside these tables, energy loss spectra are presented in the graphical form.

  5. Resistência das plantas a moléstias fúngicas Plant resistance to fungal diseases

    Directory of Open Access Journals (Sweden)

    Rodrigo Rodrigues Matiello

    1997-03-01

    Full Text Available As plantas apresentam resistência a moléstias causadas por fungos patogênicos em função da sua constituição genética e de falares do ambiente. Em programas de melhoramento genético, um dos principais objetivos é a obtenção de plantas com alto potencial de rendimento e com resistência às moléstias. Para aumentar a eficiência da seleção, é importante o conhecimento das bases genéticas da resistência. Como a variabilidade dos fungos é bastante ampla, há a necessidade da utilização de estratégias que reduzam a probabilidade de estabelecimento do patógeno nas culturas.Plant resistance to fungic diseases is due to their genetic constitution and environmental factors. In breeding programs, one of the main objectives is to obtain high grainyield potenlial plants with resistance to the prevalent diseases. Knowledge of genetic basis of resistance is importam to increase selection efficiency and due to the wide variability of fungi there is the necessity of using different strategies to reduce the probability of pathogen estabtishment in crops.

  6. 3d-modelling workflows for trans-nationally shared geological models - first approaches from the project GeoMol

    Science.gov (United States)

    Rupf, Isabel

    2013-04-01

    To meet the EU's ambitious targets for carbon emission reduction, renewable energy production has to be strongly upgraded and made more efficient for grid energy storage. Alpine Foreland Basins feature a unique geological inventory which can contribute substantially to tackle these challenges. They offer a geothermal potential and storage capacity for compressed air, as well as space for underground storage of CO2. Exploiting these natural subsurface resources will strongly compete with existing oil and gas claims and groundwater issues. The project GeoMol will provide consistent 3-dimensional subsurface information about the Alpine Foreland Basins based on a holistic and transnational approach. Core of the project GeoMol is a geological framework model for the entire Northern Molasse Basin, complemented by five detailed models in pilot areas, also in the Po Basin, which are dedicated to specific questions of subsurface use. The models will consist of up to 13 litho-stratigraphic horizons ranging from the Cenozoic basin fill down to Mesozoic and late Paleozoic sedimentary rocks and the crystalline basement. More than 5000 wells and 28 000 km seismic lines serve as input data sets for the geological subsurface model. The data have multiple sources and various acquisition dates, and their interpretations have gone through several paradigm changes. Therefore, it is necessary to standardize the data with regards to technical parameters and content prior to further analysis (cf. Capar et al. 2013, EGU2013-5349). Each partner will build its own geological subsurface model with different software solutions for seismic interpretation and 3d-modelling. Therefore, 3d-modelling follows different software- and partner-specific workflows. One of the main challenges of the project is to ensure a seamlessly fitting framework model. It is necessary to define several milestones for cross border checks during the whole modelling process. Hence, the main input data set of the

  7. Avifauna en sistemas silvopastoriles en el Corredor Biológico Mesoamericano, Tabasco, México

    Directory of Open Access Journals (Sweden)

    Noel González-Valdivia

    2014-09-01

    Full Text Available Los sistemas silvopastoriles contienen rasgos ecológicos y económicos que contribuyen con la conservación de comunidades florísticas y faunísticas que en ellas se desarrollan. Entre otras comunidades faunísticas se encuentra la avifauna la cual es un grupo representativo de las alteraciones del habitat, tanto a nivel específico como grupo functional. Con el objetivo de atender la iniciativa de Corredor Biológico Mesoamericano en Tabasco, México, se analizó la diversidad de aves en dos sistemas silvopastoriles: árboles dispersos en potreros (ADP y árboles en cercos o linderos (AL. Se aplicó el método de punto de conteo de radio fijo en tres sitios prioritarios del CBM de Tabasco, ubicados en los municipios: Huimanguillo, Tacotalpa y Tenosique. Los datos provienen de las estaciones seca y húmeda del 2011. Se registraron 2 084 aves de 154 especies (79-89% del esperado y 36 familias. Se detectaron 92, 87 y 85 especies, incluyendo 35 protegidas, con 23, 19 y 16 en Huimanguillo, Tacotalpa y Tenosique, respectivamente. Todos los sitios mostraron alta diversidad (H’≥3.20, baja dominancia de especies (D≥0.08 y alta equidad (J≥0.77. La composición de especies mostró diferencias entre sitios, con mayor similitud entre Tacotalpa y Tenosique. Diez especies pueden considerarse características de los sitios. Aunque en los sistemas silvopastoriles se refugian aves protegidas, el componente arbóreo es poco diverso y corresponde a especies de sucesión secundaria temprana, lo que limita a las aves frugívoras y especialistas de bosque. Diversificar estos sistemas con árboles nativos puede mejorar estos hábitats complementarios e incrementar la conectividad del paisaje para cumplir con los objetivos del CBM en la conservación de la biodiversidad y provisión de bienes a las poblaciones humanas.

  8. La noción de sujeto en Antropología Biológica

    Directory of Open Access Journals (Sweden)

    Lahitte, Héctor Blas

    2005-01-01

    Full Text Available Definir la noción de sujeto en antropología biológica alude-al menos-a dos preguntas: ¿qué es un ser vivo? ¿a partir de qué condición/es nos hacemos humanos?. Sin entrar a discutir la inmensa variedad de posiciones a propósito del entendimiento de lo humano; sí podemos afirmar que todo intento de comprensión fundado en la interacción de naturaleza y entorno posee al menos sentido común. Cualquier batalla que destaque exageradamente la importancia de uno u otro resultará agotadoramente inútil. Mi punto de vista, no respalda ni al nativismo partidario de la herencia ni al ambientalismo partidario del entorno. Creo que herencia y ambiente son dos perspectivas de un saber único para explicar nuestra condición. Los seres humanos somos seres sociales, vivimos nuestro ser cotidiano, en permanente relación con el ser de otros. Al mismo tiempo somos el devenir de experiencias individuales intransferibles. Como seres vivos somos sistemas determinados estructuralmente: todo ocurre en nosotros, a modo de cambios producidos como resultado de nuestra organización interna, o como condición de nuestras interacciones con el medio. -La conducta de cualquier ser vivo es adecuada si sus cambios ocurren en congruencia con los cambios del medio. -Nuestro llegar a ser es siempre resultado de dos procesos: conservación y variación. El dominio social humano, posee un mecanismo fundamental; el lenguaje: surge como característica del proceso que le da origen. Nos hacemos humanos en y por el lenguaje desde él accedemos a la autobservación y autoconciencia. Como bien expresa M. Ridley: "...la naturaleza humana es una mezcla de los principios de Darwin, la herencia de Galton, los instintos de James, los genes de De Vries, los reflejos de Pavlov, las asociaciones de Watson, la historia de Kraepelin, la experiencia positiva de Freud, la cultura de Boas, la división del trabajo de Durkheim, el desarrollo de Piaget y la creación de lazos afectivos de

  9. Excitação e fotoabsorção de moléculas interestelares no ultra violeta

    Science.gov (United States)

    Rodrigues, A. M. F.; Boechat-Roberty, H. M.; Souza, G. B.; Turci, C. C.

    2003-08-01

    O estudo dos processos de excitação, fotoabsorção e ionização molecular nas nuvens interestelar, permite a análise dos processos químicos, como a formação e destruição de moléculas, até mesmo daquelas que dão origem à vida. Acredita-se que as moléculas como CS2, NH3, CO2 e N2O estão presentes nas nuvens onde são formadas as estrelas e seus sistemas planetários. Estas moléculas são congeladas na superfície de objetos densos, como aqueles encontrados na Nuvem de Oort do nosso Sistema Solar. Quando esses objetos (cometas) desprendem-se dessa região, aproximam-se do Sol, sofrem a interação da radição Ultra Violeta (UV), passam à fase gasosa e são bombardeados por íons e elétrons presentes nos ventos solares. Obtivemos os espectros de fotoabsorção do CS2, NH3 e N2O na faixa do UV, convertendo espectros de Perda de Energia de Elétrons, medidos no menor ângulo de espalhamento e na energia de impacto de 1000 eV. Destes espectros determinamos os valores de força de oscilador (f) e de seção de choque absolutas na faixa de 500 a 2000 Å. Para tal, estudamos e comparamos dois diferentes métodos de conversão. Neste trabalho também geramos muitos dados moleculares como, valores absolutos de seção de choque elástica em função do ângulo de espalhamento e a distribuição de força do oscilador generalizada (df/dE) em função da energia de excitação para diversos ângulos de espalhamento. Comparando nossos espectros de fotoabsorção com o espectro da atmosfera de Júpiter, obtido pelo Telescópio Espacial Hubble, logo após o impacto do cometa Shoemaker-Levy 9, confirmamos a presença do CS2 e da amônia.

  10. Ctla-4, una molécula que inhibe la activación de los linfocitos T

    OpenAIRE

    Cecilia Fernández-Ponce; Juan David Hernández-Martínez; Carlos Silvera-Redondo

    2006-01-01

    CTLA-4 (Antígeno-4 asociado al Linfocito T Citotóxico) es una molécula expresada en la superficie de la mayoría de los linfocitos T activados. Su función es regular la homeostasis y la tolerancia periférica inmunológica inhibiendo la activación de los linfocitos T. Dos mecanismos han sido postulados para mediar la inhibición por CTLA-4 de la respuesta de los linfocitos T: Señalización negativa y antagonismo competitivo de la vía de coestimulación mediada por CD28/B7. Las diferente...

  11. Preliminary analysis of construction of the test drift in boom clay at Mol using plasticity solutions and finite elements

    International Nuclear Information System (INIS)

    Mair, R.J.; Taylor, R.N.; Higgins, K.G.; Potts, D.M.

    1989-01-01

    Analyses have been undertaken on an advancing tunnel heading at great depth in a clay formation corresponding to the test drift construction at Mol. Belgium. Simplifying assumptions enable plasticity solutions to be used to model the behaviour of a tunnel heading in a linear elastic-perfectly plastic soil. Finite element analysis with the same soil model has been undertaken of the test drift construction, assuming axisymmetric conditions. The results are compared with the plasticity solutions and with the measurements of lining stresses, soil movements and pore pressures by SCK/CEN. Good agreement is obtained between the plasticity solutions and finite element analysis. The measured immediate build-up of stress on the linings is well-predicted and reasonable agreement is obtained between predicted and measured soil movements. The measured pore-pressure changes are poorly predicted by the analyses

  12. MLP Tools: a PyMOL plugin for using the molecular lipophilicity potential in computer-aided drug design

    Science.gov (United States)

    Oberhauser, Nils; Nurisso, Alessandra; Carrupt, Pierre-Alain

    2014-05-01

    The molecular lipophilicity potential (MLP) is a well-established method to calculate and visualize lipophilicity on molecules. We are here introducing a new computational tool named MLP Tools, written in the programming language Python, and conceived as a free plugin for the popular open source molecular viewer PyMOL. The plugin is divided into several sub-programs which allow the visualization of the MLP on molecular surfaces, as well as in three-dimensional space in order to analyze lipophilic properties of binding pockets. The sub-program Log MLP also implements the virtual log P which allows the prediction of the octanol/water partition coefficients on multiple three-dimensional conformations of the same molecule. An implementation on the recently introduced MLP GOLD procedure, improving the GOLD docking performance in hydrophobic pockets, is also part of the plugin. In this article, all functions of the MLP Tools will be described through a few chosen examples.

  13. Adhesive evaluation of thin films of LARC-TPI and LARC-TPI with 5 mol % ODA

    Science.gov (United States)

    Progar, D. J.

    1986-01-01

    A commercially available LARC-TPI film and an experimentally prepared film of LARC-TPI with 5 mol % of 4,4'-oxydianiline (ODA), designated as LARC-TPI/ODA in the report, supplied by Mitsui Toatsu Chemicals, Incorporated (MTCI), Japan, were evaluated as thermoplastic adhesive films for bonding Ti-6Al-4V. The LARC-TPI/ODA had been shown by MTCI to possess more flow than thermoplastic LARC-TPI and was, therefore, evaluated and compared to the LARC-TPI. Lap shear strength was used to evaluate the materials as adhesives. They were characterized after fracture by determining the glass transition temperature, Tg. The mode of failure was also reported. Thermal exposure at 204C for 500 and 1000 hrs and a 72-hour water-boil were conducted on lap shear specimens prepared with the two adhesive films. Lap shear tests were conducted at RT, 177C, 204C, and 232C before and after exposures.

  14. MLP Tools: a PyMOL plugin for using the molecular lipophilicity potential in computer-aided drug design.

    Science.gov (United States)

    Oberhauser, Nils; Nurisso, Alessandra; Carrupt, Pierre-Alain

    2014-05-01

    The molecular lipophilicity potential (MLP) is a well-established method to calculate and visualize lipophilicity on molecules. We are here introducing a new computational tool named MLP Tools, written in the programming language Python, and conceived as a free plugin for the popular open source molecular viewer PyMOL. The plugin is divided into several sub-programs which allow the visualization of the MLP on molecular surfaces, as well as in three-dimensional space in order to analyze lipophilic properties of binding pockets. The sub-program Log MLP also implements the virtual log P which allows the prediction of the octanol/water partition coefficients on multiple three-dimensional conformations of the same molecule. An implementation on the recently introduced MLP GOLD procedure, improving the GOLD docking performance in hydrophobic pockets, is also part of the plugin. In this article, all functions of the MLP Tools will be described through a few chosen examples.

  15. Structure and optical homogeneity of LiNbO3:Zn (0.03–4.5 mol.%) crystals

    International Nuclear Information System (INIS)

    Sidorov, Nikolay; Tepljakova, Natalja; Gabain, Aleksei; Yanichev, Aleksander; Palatnikov, Mikhail

    2014-01-01

    Structure and optical homogeneity of LiNbO 3 :Zn (0.03–4.5 mol.%) crystals were searched by photoinduced light scattering and by Raman spectroscopy. The photorefractive effect depends on Zn 2+ concentration nonmonotonically. Decrease of photorefractive effect is explained by decrease of structure defects with localized electrons. The Zn 2+ cations replace structure defects Nb Li and Li Nb , trapping levels appear near the bottom of the conduction band and photo electrons recombine with emission under laser radiation. By the Raman spectra the area of the high structure order is found. In this area the own alternation, the alternation of impurity cations and the vacancies along the polar axis is almost perfect

  16. Respuesta eléctrica de Nanocristales ZrO2-3mol% Y2O3

    Directory of Open Access Journals (Sweden)

    Domínguez-Rodríguez, A.

    1999-12-01

    Full Text Available The evolution of microstructure and electrical behaviour of nanocrystalline ZrO2 - 3mol% Y2O3 samples with respect to thermal cycles have been investigated. The sample, that initially consisted of 77.8%wt of tetragonal and 22.3%wt monoclinic phases, eliminates the tetragonal phase by means of a low temperature t-m phase transformation in which the content of monoclinic phase reaches 96%wt. This transformation affects significantly the electrical properties of the sample, increasing the grain boundary resistivity in successive thermal cycles, whereas the bulk contribution remains unchanged. The growth of the grain boundary resistivity is believed to be due to the presence of microcracks that accommodate the larger size of monoclinic grains.Se ha estudiado la evolución con los tratamientos térmicos de la microestructura y el comportamiento eléctrico de nanocristales ZrO2 - 3mol% Y2O3. La muestra, que inicialmente estaba compuesta por 77.8%wt de fase tetragonal y 22.3%wt de fase monoclínica, sufre una transformación de fase t´m a baja temperatura en la que el porcentaje de fase monoclínica alcanza el 96%wt. Esta transformación afecta significativamente a las propiedades eléctricas del material de forma que la contribución del borde de grano ve incrementada su resistividad en los sucesivos ciclos térmicos, mientras que la contribución del grano no cambia significativamente Creemos que el aumento de resistividad del borde de grano esté relacionado con la aparición de microfisuras que acomodan el mayor tamaño de los granos monoclínicos.

  17. Potentiometric study of rhenium (V) complex formation with 4-methyl-1.2.4-triazol thiol-5 in the medium of 7 mol/l HBr

    International Nuclear Information System (INIS)

    Amindzhanov, A.A.; Safarmamadov, S.M.

    1992-01-01

    Present article is devoted to potentiometric study of rhenium (V) complex formation with 4-methyl-1.2.4-triazol thiol-5 in the medium of 7 mol/l HBr. The function of formation of oxo bromine-4-methyl-1.2.4-triazol thiol complexes of rhenium (V) in the medium of 5 mol/l HBr at 273 K temperature was determined. The function of formation of oxo bromine-4-methyl-1.2.4-triazol thiol complexes of rhenium (V) in the medium of 5 mol/l HBr at 298 K temperature was determined as well.

  18. El enemigo olvidado: la ricina y su amenaza como arma biológica a los Estados Unidos

    OpenAIRE

    Martínez Pantoja, Yadira Ixchel

    2005-01-01

    El riesgo latente de un ataque terrorista con armas nucleares fue el centro de atención de todas las agencias de seguridad de los Estados Unidos y las naciones aliadas durante la Guerra Fría. Sin embargo, los avances en la genética y la biotecnología han puesto en manos terroristas nuevas armas para vulnerar la seguridad nacional estadounidense: los agentes biológicos. Además, los ataques del 11 de Septiembre modificaron el concepto de seguridad nacional forzando a los depar...

  19. Entendendo o papel de marcadores biológicos no câncer de pulmão

    OpenAIRE

    CAPELOZZI VERA LUIZA

    2001-01-01

    Marcadores biológicos são componentes celulares, estruturais e bioquímicos, que podem definir alterações celulares e moleculares tanto em células normais quanto aquelas associadas a transformação maligna. Podem ser de dois tipos: 1) marcadores intermediários, que medem alterações celulares e moleculares antes do aparecimento da malignidade; 2) marcadores diagnósticos, presentes em associação com a malignidade. O processo de identificação e validação para uso clínico do marcador tem diversas e...

  20. Teoría de agencia: una revisión del origen biológico del delito

    OpenAIRE

    Araya, Iván; Crespo, Fernando A.

    2016-01-01

    Este trabajo aborda la causa del conflicto entre principal y agente, buscando respuestas en las disciplinas de la biología, particularmente en las ciencias de la antropología criminal. Se ha realizado un ensamble entre los hallazgos de la teoría de agencia y la teoría de los delitos de cuello blanco, reinterpretando las actuaciones del agente desde su configuración entre instinto, herencia y personalidad. Los resultados en las ciencias biológicas podrían aportar nuevas interpretac...

  1. Salud del suelo: en la búsqueda de un indicador biológico de sustentabilidad

    OpenAIRE

    Ortíz, Jimena; Faggioli, Valeria; Ullé, Jorge A.

    2015-01-01

    Los indicadores de calidad de suelo han sido motivo de numerosas investigaciones en los últimos años. Sin embargo, la complejidad en la interpretación de los resultados de laboratorio dificulta su implementación tanto para los productores como para el asesor. Es por ello que el objetivo de este trabajo fue evaluar el comportamiento de indicadores biológicos de suelo sobre diferentes intensidades de manejo orgánico, con énfasis en el coeficiente metabólico. Se analizó un set de datos provenien...

  2. Poliquetos (Annelida: Polychaeta) como indicadores biológicos de contaminación marina: casos en Colombia

    OpenAIRE

    Vanessa Fernández Rodríguez; Mario H. Londoño Mesa

    2015-01-01

    El concepto de Indicador Biológico ha sido usado sin mayor precaución al momento de emplear ciertos organismos en programas de monitoreo ambiental, lo cual genera confusión. Uno de los objetivos de este trabajo fue resaltar la importancia en la consolidación de una definición precisa de este concepto a partir de información disponible, proponiendo una definición para el mismo. Las características ecológicas de los poliquetos permiten que, al estar en contacto permanente con diferentes tipos d...

  3. Estudio de procesos biológicos de tratamiento de residuos basado en la modelización

    OpenAIRE

    Magrí Aloy, Albert; Flotats Ripoll, Xavier; Solé-Mauri, Francina; Illa Alibes, Josep

    2006-01-01

    Los modelos matemáticos se han convertido en una herramienta básica para aumentar la comprensión de los procesos biológicos relacionados con el tratamiento de residuos orgánicos. En este sentido, la modelación permite crear un lenguaje de comunicación común, orientar el diseño experimental, evaluar resultados, contrastar hipótesis, revelar relaciones entre variables, prever la evolución de sistemas y, en definitiva, diseñar estrategias de gestión y tratamiento op...

  4. Estudios fisicoqu??micos y biol??gicos de un nuevo derivado de la eritromicina: el folato de eritromicina

    OpenAIRE

    Manna, P.K.; Kumaran, V.; Mohanta, G.P.

    2005-01-01

    Se prepar?? un derivado nuevo de la eritromicina, el folato de eritromicina, y se evaluaron sus propiedades fisicoqu??micas y biol??gicas. El derivado presenta una buena solubilidad en metanol, etanol y propileno glicol. Los valores de los coeficientes de partici??n, que fueron 1,12 y 1,10 en sistemas de cloroformo/agua y octanol/agua respectivamente, indican que probablemente se distribuya bien in vivo. La potencia in vitro del derivado, 716 ??g/mg, es mayor que la de derivados e...

  5. Características neuromotoras e biopsicossocias de lactentes com histórico de risco biológico

    Directory of Open Access Journals (Sweden)

    Joyce Ribeiro

    Full Text Available INTRODUÇÃO: Os avanços tecnológicos têm permitido maior sobrevivência de recém-nascidos de risco. No entanto, os fatores de risco biológico aos quais essas crianças foram expostas fazem com que tenham maior chance de apresentar atrasos no desenvolvimento. OBJETIVO: O presente estudo objetivou avaliar as características neuromotoras e biopsicossociais de lactentes com histórico de risco biológico. METODOLOGIA: A amostra foi constituída de 25 lactentes com idade desde o nascimento a dois meses de vida, com registro de nascimento na cidade de Fraiburgo, SC. Os fatores de risco biológicos presentes foram: prematuridade, baixo peso, incompatibilidade sanguínea, necessidade de reanimação, intubação, permanência em O2, hipertensão pulmonar leve, icterícia, desconforto respiratório, infecção neonatal, anemia neonatal, tabagismo materno, trauma durante o parto, cardiopatia e má-formação de traqueia. Foram utilizados os seguintes instrumentos: cartão de saúde da criança, formulário biopsicossocial e Neonatal Behavioral Assesment Scale. RESULTADOS: Foi possível constatar, em relação às condições biopsicossociais, prevalência de mães casadas, primigestas, com ensino superior completo, exercendo atividade extra-lar e com idade média de 28 anos na ocasião do parto. Verificou-se casos de exposição do feto ao tabagismo materno, mães acometidas por anemia, hipertensão e hipotensão durante o período gestacional. Em relação ao desenvolvimento neuromotor, os lactentes apresentaram desempenho esperado para crianças de risco em relação à habituação, regulação e reflexos. Em relação à socialização, organização e sistema autônomo, constatou-se desempenho inferior ao de demais crianças de risco. CONCLUSÃO: Os achados deste estudo demonstram que crianças com histórico de risco biológico podem apresentar comprometimento em relação a aspectos do desenvolvimento neuromotor.

  6. Optimización de procesos biológicos para la eliminación de N2O

    OpenAIRE

    Arvelo Yagua, Ilan Alexander

    2014-01-01

    El N2O es uno de los principales gases de efecto invernadero, ocupando el tercer lugar de las emisiones globales medidas en toneladas de CO2 equivalente, siendo la principal fuente antropogénica el uso de fertilizantes nitrogenados. Actualmente la tecnología para su tratamiento por mecanismos biológicos presenta escasos avances, y debido a ello surge la necesidad de la investigación experimental para el conocimiento de parámetros operacionales y de diseño que permitan el uso óptimo de reac...

  7. Antártica: fuente de recursos biológicos para la biotecnología nacional

    OpenAIRE

    Blamey, J.

    2008-01-01

    En diciembre del 2007, Innova-Chile de CORFO aprobó el financiamiento del proyecto “Antártica: Fuente de recursos biológicos para la biotecnología nacional”. Este proyecto tiene como fin crear una plataforma que facilite el acceso a los recursos antárticos de microorganismos y plantas, de una forma que permitirá su valorización para la biotecnología chilena y su desarrollo científico en el área. Por sus únicas condiciones de aislamiento y bajas temperaturas, la Antártica alberga un potencial ...

  8. Reator biológico com membrana (MBR) aplicado ao tratamento de esgotos gerados por unidades residenciais unifamiliares

    OpenAIRE

    Pereira, Anne Relvas

    2017-01-01

    A escolha da tecnologia apropriada para o tratamento de esgoto doméstico tem sido uma preocupação crescente devido ao interesse em proporcionar um efluente de melhor qualidade, tanto para manutenção da qualidade ambiental como para produzir água para reúso. O objetivo desta pesquisa foi o de avaliar a aplicabilidade do processo de Reator Biológico com Membrana (MBR) a sistemas provenientes de unidades residenciais unifamiliares de esgotos no Brasil e as condições em que esse processo seria re...

  9. Aspectos étnicos, biológicos e químicos de Senna occidentalis (Fabaceae)

    OpenAIRE

    T. M. KANEKO; S. KIYOTA; M. LOMBARDO

    2009-01-01

    Senna occidentalis (sin. Cassia occidentalis) é um arbusto perene nativo da América do Sul e distribuída em regiões tropicais ao redor do mundo, frequentemente contaminando pastos e culturas de cereais. Inúmeros estudos demonstraram que esta planta é tóxica para animais. Na medicina popular, tribos americanas, africanas e indianas usam preparações da S. occidentalis como tônico, estomáquico, febrífugo, laxante e antimicrobiano. Diversas propriedades biológicas da espécie já foram comprovadas,...

  10. Perfil fitoquímico de materiais biológicos usados em dessalinizador caseiro de água salobra

    OpenAIRE

    Costa, Tadeu Antônio de Campos

    2011-01-01

    116f. Para tornar potáveis águas salobras ou salinas, é necessário fazer a dessalinização, processo que normalmente exige alto investimento e recursos tecnológicos complexos para a produção em larga escala. Uma solução simples e eficiente para as comunidades do Semi Árido pode ser a dessalinização de água salobra pelo contato com materiais biológicos, através do uso de dispositivos caseiros contendo, por exemplo, sementes que adsorvam parte do sal da água, reduzindo a salini...

  11. Estudo fitoquímico e biológico das folhas de Banisteriopsis argyrophylla (A. Juss.) B. Gates (Malpighiaceae)

    OpenAIRE

    Oliveira, Daiane Maria

    2016-01-01

    O aumento da resistência dos fungos em relação aos medicamentos existentes no mercado e os efeitos colaterais apresentados por alguns compostos com propriedades antioxidantes e inibidores enzimáticos, em especial contra a α-amilase e α-glicosidase, torna-se necessária à descoberta de novos compostos com potencial biológico. Neste contexto os produtos naturais podem ser uma fonte importante para a descoberta de novas arquiteturas moleculares ativas. Assim, este trabalho teve como objetivos ava...

  12. haematological, lipid profile and other biochemical parameters

    African Journals Online (AJOL)

    hi-tech

    2006-01-01

    Jan 1, 2006 ... HAEMATOLOGICAL, LIPID PROFILE AND OTHER BIOCHEMICAL PARAMETERS IN NORMAL AND HYPERTENSIVE SUBJECTS AMONG THE ... Hypertensive individuals had significantly higher serum sodium, chloride and calcium levels but a .... exposed to urbanisation and associated changes in diet,.

  13. Biochemical and immunohistochemical characterisation of mucins in ...

    African Journals Online (AJOL)

    Biochemical and immunohistochemical characterisation of mucins in 8 cases of colonic disease - a pilot study. N Chirwa, A Mall, M Tyler, B Kavin, P Goldberg, JEJ Krige, Z Lotz, D Khan, D Govender, A Hunter ...

  14. Biochemical, haematological and morphological variations in ...

    African Journals Online (AJOL)

    Biochemical, haematological and morphological variations in juvenile Clarias gariepinus exposed to Carbendazim® fungicide. ... protein levels and neutrophil numbers. Consequently, the use and environmental concentrations of CBZ should be closely monitored to safeguard fish health condition in aquatic ecosystems.

  15. Morphological, physiological and biochemical studies on Pyricularia ...

    African Journals Online (AJOL)

    SARAH

    2014-02-28

    Magnaporthe grisea) causes significant yield loss in Ethiopia. This study was conducted to isolate, identify and characterize the pathogen (using morphological, physiological and biochemical methods). Methodology and ...

  16. Raman spectroscopic biochemical mapping of tissues

    Science.gov (United States)

    Stone, Nicholas; Hart Prieto, Maria C.; Kendall, Catherine A.; Shetty, Geeta; Barr, Hugh

    2006-02-01

    Advances in technologies have brought us closer to routine spectroscopic diagnosis of early malignant disease. However, there is still a poor understanding of the carcinogenesis process. For example it is not known whether many cancers follow a logical sequence from dysplasia, to carcinoma in situ, to invasion. Biochemical tissue changes, triggered by genetic mutations, precede morphological and structural changes. These can be probed using Raman or FTIR microspectroscopy and the spectra analysed for biochemical constituents. Local microscopic distribution of various constituents can then be visualised. Raman mapping has been performed on a number of tissues including oesophagus, breast, bladder and prostate. The biochemical constituents have been calculated at each point using basis spectra and least squares analysis. The residual of the least squares fit indicates any unfit spectral components. The biochemical distribution will be compared with the defined histopathological boundaries. The distribution of nucleic acids, glycogen, actin, collagen I, III, IV, lipids and others appear to follow expected patterns.

  17. A Program on Biochemical and Biomedical Engineering.

    Science.gov (United States)

    San, Ka-Yiu; McIntire, Larry V.

    1989-01-01

    Presents an introduction to the Biochemical and Biomedical Engineering program at Rice University. Describes the development of the academic and enhancement programs, including organizational structure and research project titles. (YP)

  18. Short Report Biochemical derangements prior to emergency ...

    African Journals Online (AJOL)

    diagnoses frequently associated with biochemical derangement. A substantial proportion displayed clinically significant derangements in their blood tests. These derangements can result in anorexia, nausea, muscle weakness, paralytic ileus, and ...

  19. Biochemical genetics of some Indian fishes

    Digital Repository Service at National Institute of Oceanography (India)

    Menezes, M.R.; Qasim, S.Z.

    Studies on biochemical genetics of fishes, using electrophoretic methods, are relatively of recent origin. Earlier serum and eye lens protein were used to identify marine populations. This technique showed that closely related species have...

  20. [Biochemical diagnostics of fatal opium intoxication].

    Science.gov (United States)

    Papyshev, I P; Astashkina, O G; Tuchik, E S; Nikolaev, B S; Cherniaev, A L

    2013-01-01

    Biochemical diagnostics of fatal opium intoxication remains a topical problem in forensic medical science and practice. We investigated materials obtained in the course of forensic medical expertise of the cases of fatal opium intoxication. The study revealed significant differences between myoglobin levels in blood, urine, myocardium, and skeletal muscles. The proposed approach to biochemical diagnostics of fatal opium intoxication enhances the accuracy and the level of evidence of expert conclusions.

  1. Improving Marine Ecosystem Models with Biochemical Tracers

    Science.gov (United States)

    Pethybridge, Heidi R.; Choy, C. Anela; Polovina, Jeffrey J.; Fulton, Elizabeth A.

    2018-01-01

    Empirical data on food web dynamics and predator-prey interactions underpin ecosystem models, which are increasingly used to support strategic management of marine resources. These data have traditionally derived from stomach content analysis, but new and complementary forms of ecological data are increasingly available from biochemical tracer techniques. Extensive opportunities exist to improve the empirical robustness of ecosystem models through the incorporation of biochemical tracer data and derived indices, an area that is rapidly expanding because of advances in analytical developments and sophisticated statistical techniques. Here, we explore the trophic information required by ecosystem model frameworks (species, individual, and size based) and match them to the most commonly used biochemical tracers (bulk tissue and compound-specific stable isotopes, fatty acids, and trace elements). Key quantitative parameters derived from biochemical tracers include estimates of diet composition, niche width, and trophic position. Biochemical tracers also provide powerful insight into the spatial and temporal variability of food web structure and the characterization of dominant basal and microbial food web groups. A major challenge in incorporating biochemical tracer data into ecosystem models is scale and data type mismatches, which can be overcome with greater knowledge exchange and numerical approaches that transform, integrate, and visualize data.

  2. Actividades biológicas del extracto acuoso de la esponja Aplysina lacunosa (Porifera: Aplysinidae

    Directory of Open Access Journals (Sweden)

    Arda Kazanjian

    2006-12-01

    . lacunosa has a protein with a molar mass of about 43 000 Daltons which is capable of agglutinating human erythrocytes of the blood groups A, B, and O in a strong and unspecific mode. The assayed samples did not evidence any hemolysing activity. As for the antibacterial assay, only the aqueous extract was able to inhibit the growth of Enterococcus faecalis, Bacillus cereus, Escherichia coli, and Salmonella enteritidis, with inhibition halos of 24, 20, 24, and 22 mm, respectively. None of the samples exhibited antifungal activity. The chemical analysis of the aqueous extract revealed the presence of several secondary metabolites. It is presumed that its hemagglutinating activity is mediated by agglutinative proteins. The antibacterial activity could be attributed to the presence of saponins, alkaloids, tannins, and polyphenols, which are highly antimicrobial compounds. Poriferans are a rich source of bioactive compounds that can be used in the development of new drugs potentially useful in medicine. Rev. Biol. Trop. 54 (Suppl. 3: 189-200. Epub 2007 Jan. 15.

  3. Residual dysglycemia when at target HbA(1c) of 7% (53mmol/mol) in persons with type 2 diabetes.

    Science.gov (United States)

    Monnier, L; Colette, C; Dejager, S; Owens, D

    2014-06-01

    To understand the composition of the residual dysglycemia when HbA1c is between 6.5% (48mmol/mol) and 7% (53mmol/mol), representing the definition of diabetes and the recommended treatment goal, respectively. One hundred persons with type 2 diabetes and a HbA1cmol), treated with diet alone and/or oral hypoglycemic agents underwent continuous glucose monitoring (CGM) and were further divided into two subgroups 1 (n=50) and 2 (n=50) according to whether the HbA1c was mol) or 6.5-6.9% (48-52mmol/mol), respectively. A similar analysis was performed in those on diet alone: subgroups A (n=34, HbA1cmol) and B (n=10, HbA1c 6.5-6.9%, 48-52mmol/mol). The residual dysglycemia determined from the CGM was assessed using glucose exposures defined as areas under curves (AUCs) and mean glucose values. Averaged 2-h postprandial glucose value (averaged PPG, mmol/L, mean±SD) and postprandial glucose exposure (AUCpp, mean±SD, mmol·L(-1)·h) were significantly higher in subgroup 2 (mean HbA1c=6.7%, 50mmol/mol) than in subgroup 1 (mean HbA1c=6.0%, 42mmol/mol): averaged PPG=8.1±1.3 versus 7.3±1.3mmol/L (pmol) inclusive is mainly due to remnant abnormal postprandial glucose excursions. Consequently, HbA1cmol) is an achievable goal with therapeutic measures aimed at reducing postmeal glucose when the HbA1c is at 7% (53mmol/mol). Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  4. Las ciperáceas (Cyperaceae de la Estación Biológica La Selva, Costa Rica

    Directory of Open Access Journals (Sweden)

    Jorge Gómez-Laurito

    2009-11-01

    Full Text Available La familia Cyperaceae es cosmopolita e incluye unos 115 géneros y 3600 especies. Costa Rica tiene 25 géneros y 210 especies. Aquí presento claves y descripciones para las 34 especies (10 géneros que ocurren en la Estación Biológica La Selva, Costa Rica. El género Cyperus está representado por 12 especies, seguido por Rhynchospora (6, Scleria (5, Calyptocarya (2, Eleocharis (2, Kyllinga (2, Mapania (2, Hypolytrum (1 y Lipocarpha (1. Oxycaryum cubense (Poepp & Kunth Palla, ha sido colectada en los alrededores y eventualmente podría ser encontrada en la Estación.The Cyperaceae of La Selva Biological Station, Costa Rica. The family Cyperaceae is cosmopolitan and includes some 115 genera and 3600 species. Costa Rica has 25 genera and 210 species. Here I present keys and descriptions for 34 species (10 genera that occur in La Selva Biological Station, Costa Rica. The genus Cyperus is represented by 12 species, followed by Rhynchospora (6, Scleria (5, Calyptocarya (2, Eleocharis (2, Kyllinga (2, Mapania (2, Hypolytrum (1 and Lipocarpha (1. Oxycaryum cubense (Poepp & Kunth Palla, has been collected in the vicinity and could eventually be found at the Station. Rev. Biol. Trop. 57 (Suppl. 1: 93-110. Epub 2009 November 30.

  5. Adoção suficientemente boa: experiência de um casal com filhos biológicos

    Directory of Open Access Journals (Sweden)

    Livia Kusumi Otuka

    Full Text Available A partir de um estudo de caso, aborda-se a experiência de adoção de uma criança de quatro meses de idade por um casal com três filhos biológicos. Os dados obtidos por entrevistas semiestruturadas mostraram que a parentalidade adotiva foi experienciada em meio a temores/fantasias de perda/roubo da criança adotada. Isso pode ser associado ao modo como foi realizada a adoção, sem os cuidados preconizados pelo Estatuto da Criança e do Adolescente e a nova lei de adoção. O processo de adoção figurou para os adotantes como fonte de intensa carga emocional, permeada por sentimentos ambivalentes que merecem atenção dos profissionais da Psicologia. Os resultados ilustram a necessidade de atenção psicossocial-jurídica a adotantes, adotado e família biológica do adotado.

  6. O neolamarckismo de Edward Drinker Cope e a ideia de progresso biológico no processo evolutivo

    Directory of Open Access Journals (Sweden)

    Felipe Faria

    Full Text Available A teoria neolamarckista de Edward Cope operava com um mecanismo alternativo à seleção natural. Acréscimos ou decréscimos dos estágios ontogênicos produziriam características que poderiam ser geradas e integradas ao organismo por meio da herança de caracteres adquiridos. Incrementando, ou não, a complexidade corporal, tal mecanismo aumentava a capacidade adaptativa. Isso poderia ser interpretado como progresso biológico de maneira semelhante à interpretação feita por defensores da teoria sintética evolutiva. Mas, diferentemente destes últimos, o neolamarckismo relegava à seleção natural papel secundário. Este estudo esclarece o posicionamento de Cope em relação ao fenômeno do progresso biológico, assim como seu enfoque fortemente adaptacionista, propondo que essa tenha sido uma contribuição indireta à articulação da nova síntese evolutiva.

  7. As possibilidades da inclusão do aluno surdo no curso de Ciências Biológicas

    Directory of Open Access Journals (Sweden)

    Tatiane Aparecida Silva

    2016-08-01

    Full Text Available As dificuldades ou limitações na educação dos surdos sempre foram motivos de inquietações, principalmente quando se trata de incluir estes alunos no espaço acadêmico. A falta de conhecimento sobre as diferenças que separam ouvintes e surdos ainda é um tabu para as pessoas que não participam de sua cultura ou forma de comunicação. Pensando neste momento de interação é que esta pesquisa objetivou avaliar as condições e as necessidades de um aluno surdo durante as aulas teóricas e práticas do Curso de Ciências Biológicas UFU-FACIP. Participaram da pesquisa setenta e cinco discentes, dez docentes e quatro técnicos do Curso de Ciências Biológicas UFU- FACIP e cursos associados. Os resultados da pesquisa apontam aspectos importantes sobre o pouco conhecimento e convivência dos discentes, docentes e técnicos em relação aos surdos, o que torna essa interação uma novidade na Universidade. Concluindo assim que estes apresentaram grandes dificuldades para descrever o impacto da interação com um aluno surdo em sala de aula.

  8. Aspectos étnicos, biológicos e químicos de Senna occidentalis (Fabaceae

    Directory of Open Access Journals (Sweden)

    T. M. KANEKO

    2009-08-01

    Full Text Available Senna occidentalis (sin. Cassia occidentalis é um arbusto perene nativo da América do Sul e distribuída em regiões tropicais ao redor do mundo, frequentemente contaminando pastos e culturas de cereais. Inúmeros estudos demonstraram que esta planta é tóxica para animais. Na medicina popular, tribos americanas, africanas e indianas usam preparações da S. occidentalis como tônico, estomáquico, febrífugo, laxante e antimicrobiano. Diversas propriedades biológicas da espécie já foram comprovadas, tais como a antibacteriana, antifúngica, antimalárica, antitumoral e hepatoprotetora. As análises fitoquímicas evidenciaram que as antraquinonas, os flavonóides e outros derivados fenólicos são os seus principais constituintes. Esta revisão apresenta dados etnofarmacológicos, químicos e biológicos publicados na literatura sobre S. occidentalis. Palavras-chave: Senna occidentalis. Cassia occidentalis. Fabaceae. Leguminosae. Caesalpinioideae. Fedegoso.

  9. Los accidentes biológicos y caracterización del riesgo en estudiantes de enfermería

    Directory of Open Access Journals (Sweden)

    Zuly Nayibe Garavito Santos

    2012-08-01

    Full Text Available Los accidentes biológicos son un evento adverso para los estudiantes de enfermería, teniendo en cuenta que comparten el mismo escenario como todo el personal sanitario. Este riesgo está dado por la probabilidad de exposición a agentes infecciosos, principalmente los de tipo viral (hepatitis B o C, virus de inmunodeficiencia humana-VIH por exposición percutánea en su mayoría. La relación estrecha enfermero- paciente que supone el quehacer de la profesión, incrementa el riesgo de accidentalidad con relación a otras carreras de la salud. Esta problemática ha sido estudiada en el mundo; sin embargo, no existen datos que muestren la problemática en Colombia, hallándose información al respecto para otras carreras de la salud. El objetivo de esta revisión es exponer de forma concisa y breve los accidentes biológicos más comunes y su caracterización en estudiantes de enfermería, identificando el grado de conocimiento, aptitudes y aplicación de las normas de prevención y bioseguridad de acuerdo con la bibliografía encontrada con miras a identificar estrategias que permitan mejorar la seguridad de los estudiantes en sus diferentes prácticas clínicas y comunitarias.

  10. Características biológicas del tejido adiposo: el adipocito como célula endocrina

    Directory of Open Access Journals (Sweden)

    Reyes J. Marcela, Dra.

    2012-03-01

    Full Text Available La obesidad se caracteriza por un aumento de la masa adiposa secundaria a un balance energético positivo mantenido en el tiempo. El incremento en el volumen del tejido adiposo se acompaña de otros cambios en las características biológicas habituales de éste, que se vuelve disfuncional. El depósito visceral de la grasa, la hipertrofia y cambio del perfil secretor de los adipocitos, junto con la infiltración del tejido adiposo por células inflamatorias son algunas de las características que determinan una comunicación alterada del tejido adiposo con otros órganos. Se ha planteado que la disfunción del tejido adiposo, explicaría parte de la etiopatogenia de las enfermedades metabólicas y cardiovasculares asociadas a obesidad; sujetos obesos que mantienen un tejido adiposo funcional no presentan las alteraciones metabólicas propias de la malnutrición por exceso. La modulación de las características biológicas del tejido adiposo permitiría tener un menor riesgo cardiovascular asociado a obesidad.

  11. Buffer Standards for pH Measurement of N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid (HEPES) for I = 0.16 mol.kg from 5 to 55 degrees C.

    Science.gov (United States)

    Roy, Rabindra N; Roy, Lakshmi N; Ashkenazi, Shahaf; Wollen, Joshua T; Dunseth, Craig D; Fuge, Michael S; Durden, Jared L; Roy, Chandra N; Hughes, Hannah M; Morris, Brett T; Cline, Kevin L

    2009-04-01

    The values of the second dissociation constant, pK(2) of N-(2-hydroxyethyl) piperazine-N'-2-ethanesulfonic acid (HEPES) have been reported at 12 temperatures over the temperature range 5 to 55 degrees C, including 37 degrees C. This paper reports the results for the pa(H) of eight isotonic saline buffer solutions with an I = 0.16 mol*kg(-1) including compositions: (a) HEPES (0.01 mol*kg(-1)) + NaHEPES (0.01 mol*kg(-1)) + NaCl (0.15 mol*kg(-1)); (b) HEPES (0.02 mol*kg(-1)) + NaHEPES (0.02 mol*kg(-1)) + NaCl (0.14 mol*kg(-1)); (c) HEPES (0.03 mol*kg(-1)) + NaHEPES (0.03 mol*kg(-1)) + NaCl (0.13 mol*kg(-1)); (d) HEPES (0.04 mol*kg(-1)) + NaHEPES (0.04 mol*kg(-1)) + NaCl (0.12 mol*kg(-1)); (e) HEPES (0.05 mol*kg(-1)) + NaHEPES (0.05 mol*kg(-1)) + NaCl (0.11 mol*kg(-1)); (f) HEPES (0.06 mol*kg(-1)) + NaHEPES (0.06 mol*kg(-1)) + NaCl (0.10 mol*kg(-1)); (g) HEPES (0.07 mol*kg(-1)) + NaHEPES (0.07 mol*kg(-1)) + NaCl (0.09 mol*kg(-1)); and (h) HEPES (0.08 mol*kg(-1)) + NaHEPES (0.08 mol*kg(-1)) + NaCl (0.08 mol*kg(-1)). Conventional pa(H) values, for all eight buffer solutions from 5 to 55 degrees C have been calculated. The operational pH values with liquid junction corrections, at 25 and 37 degrees C have been determined based on the NBS/NIST standard between the physiological phosphate standard and four buffer solutions. These are recommended as pH standards for physiological fluids in the range of pH 7.3 to 7.5 at I = 0.16 mol*kg(-1).

  12. Observation des cycles enzymatiques des ADN topoisomérases par micromanipulation de molécules individuelles

    Science.gov (United States)

    Strick, Terence R.; Charvin, Gilles; Dekker, Nynke H.; Allemand, Jean-François; Bensimon, David; Croquette, Vincent

    In this article, we describe single-molecule assays using magnetic traps and we applied these assays to topoisomerase enzymes which unwind and disentangle DNA molecules. First, the elasticity of single DNA molecule is characterized using the magnetic trap. We show that a twisting constraint may be easily applied and that its effect upon DNA may be measured accurately. Then we describe how the topoisomerase activity may be observed at the single-molecule level giving direct access to the important biological parameters of the enzyme such as velocity and processivity. Furthermore, individual cycles of unwinding can be observed in real time. This permits an accurate characterization of the enzyme's biochemical cycle. The data treatment required to identify and analyze individual topoisomerization cycles will be presented in detail. This analysis is applicable to a wide variety of molecular motors. To cite this article: T.R. Strick et al., C. R. Physique 3 (2002) 595-618.

  13. Diagnostic moléculaire de la présence de mildiou (Plasmopara halstedii dans les semences de tournesol

    Directory of Open Access Journals (Sweden)

    Says-Lesage Véronique

    2001-05-01

    Full Text Available Le mildiou du tournesol, causé par l’oomycète Plasmopara halstedii, est une maladie potentiellement dévastatrice et économiquement importante. Du fait du risque de propagation de la maladie par les semences infectées et de l’observation croissante de l’apparition d’isolats résistants au métalaxyl, il est apparu nécessaire de mettre au point une méthode de détection du parasite dans les graines ou les tissus de la plante. Nous avons donc développé un test moléculaire pour le diagnostic de la présence du mildiou dans les semences. Plusieurs méthodes d’extraction ont été comparées pour améliorer le rendement et la qualité de l’ADN du parasite présent en faible quantité. Des échantillons de graines contaminées entières et décortiquées ont été analysés par PCR, avec des amorces spécifiques de Plasmopara halstedii. L’ADN de l’agent pathogène est toujours détecté, notamment dans les coques. Ces données sont importantes pour le développement d’un kit de diagnostic.

  14. VizieR Online Data Catalog: ExoMol Line List XXI: Nitric Oxide (NO) (Wong+, 2017)

    Science.gov (United States)

    Wong, A.; Yurchenko, S. N.; Bernath, P.; Mueller, H. S. P.; McConkey, S.; Tennyson, J.

    2017-05-01

    The states files contain lists of rovibronic states. Each state is labelled with the total angular momentum, state degeneracy, total parity, vibrational quantum number, projection of the electronic, spin and total angular momenta. Each state has a unique number, which is the number of the row in which it appears in the file. This number is the means by which the state is related to the second part of the data system, the transitions files. The lifetimes and Lande-g factors are also provided. The transition files t_*dat contain four columns: the reference number in the energy file of the upper state, that of the lower state, the Einstein A coefficient of the transition and the transition wavenumber. These entries are ordered by increasing frequency. The energy file and the transitions files are bzipped, and need to be extracted before use. The p_*.dat files contain the partition functions for the six isotopologues (0...5000K) tabulated in steps of 1K. The pf_param.dat contains unitless expansion parameters a_i of the partition functions extrapolated using the following form (Vidler & Tennyson, 2000, J. Chem. Phys., 113, 9766): log10(Q) = sumi ai [log10(T)]^i A sample programme to generate synthetic spectra from the ExoMol format line lists can be obtained at www.exomol.com. (19 data files).

  15. Properties of lithium disilicate reinforced with ZrO2 (3mol%Y2O3

    International Nuclear Information System (INIS)

    Alves, M.F.R.P.; Cossu, C.M.F.A.; Santos, C.; Simba, B.G.

    2016-01-01

    The new generation of dental ceramics based on lithium disilicate, Li 2 Si 2 O 5 , allows the production of restorative prosthetic with reduced times compared to alumina and / or zirconia (Y-TZP). A great limitation of their use is related low fracture strength of such glass-ceramics, which reduces their use in unit fixed prosthesis. In this work, lithium disilicate reinforced with 10% ZrO 2 (3-mol% Y 2 O 3 ) is characterized by relative density, crystalline phase, hardness, fracture toughness and microstructural aspects. Lithium metasilicate and tetragonal zirconia, prior to heat treatment. After thermal treatment under vacuum at 840 deg C-8min the lithium metasilicate is converted to lithium disilicate as the ZrO 2 phase remains in the tetragonal structure. This maintenance of the tetragonal phase ensures the material a good fracture toughness, reaching average values near 2MPam 1/2 , while the average hardness of 600HV. Morphological analysis of the samples indicates that ZrO 2 particles are uniformly dispersed in the matrix composed of high aspect ratio lithium disilicate grains, which contributes to the results presented.. A critical analysis of the performance of toughening mechanisms such as cracks deflection, phase transformation of ZrO 2 (T-M), residual stress between the matrix and the reinforcement are presented, discussed and compared with other ceramic materials used in dentistry restorer. (author)

  16. ExoMol line lists - XXII. The rotation-vibration spectrum of silane up to 1200 K

    Science.gov (United States)

    Owens, A.; Yachmenev, A.; Thiel, W.; Tennyson, J.; Yurchenko, S. N.

    2017-11-01

    A variationally computed 28SiH4 rotation-vibration line list applicable for temperatures up to T = 1200 K is presented. The line list, called OY2T, considers transitions with rotational excitation up to J = 42 in the wavenumber range 0-5000 cm-1 (wavelengths λ > 2 μm). Just under 62.7 billion transitions have been calculated between 6.1 million energy levels. Rovibrational calculations have utilized a new `spectroscopic' potential energy surface determined by empirical refinement to 1452 experimentally derived energy levels up to J = 6, and a previously reported ab initio dipole moment surface. The temperature-dependent partition function of silane, the OY2T line list format, and the temperature dependence of the OY2T line list are discussed. Comparisons with the PNNL spectral library and other experimental sources indicate that the OY2T line list is robust and able to accurately reproduce weaker intensity features. The full line list is available from the ExoMol data base and the CDS data base.

  17. Transferability of cucumber microsatellite markers used for phylogenetic analysis and population structure study in bottle gourd (Lagenaria siceraria (Mol.) Standl.).

    Science.gov (United States)

    Bhawna; Abdin, M Z; Arya, L; Verma, M

    2015-02-01

    Improved breeding for developing fruit quality in bottle gourd (Lagenaria siceraria (Mol.) Standl.) necessitates knowledge regarding its genetic diversity. To achieve this, a set of 108 locus-specific SSR markers has been developed in bottle gourd by cross-species transferability from 995 mapped Cucumis sativus SSR markers. During screening, 280 primer pairs amplified in the bottle gourd germplasm, which were further evaluated in a diverse set of 42 lines, resulting in 19 polymorphic, 89 monomorphic, 15 with multiple bands, and the rest 157 showed no or very non-specific amplification. The 19 polymorphic primer pairs produced a total of 54 alleles. Gene diversity, Shannon's information index, and Nei's coefficient of differentiation were calculated suggesting a moderate genetic variation at the species level. A model-based population structure analysis divided these germplasm into two subpopulations. This marker set will be applicable for evaluating the genetic structure for association mapping, DNA fingerprinting, and mounting linkage maps and will be a practical tool set for further genetics. This study provides one of the first quantitative views of population genetic variation in bottle gourd.

  18. Biochemical Removal of HAP Precursors From Coal

    Energy Technology Data Exchange (ETDEWEB)

    Olson, G.; Tucker, L.; Richards, J.

    1997-07-01

    This project addresses DOE`s interest in advanced concepts for controlling emissions of air toxics from coal-fired utility boilers. We are determining the feasibility of developing a biochemical process for the precombustion removal of substantial percentages of 13 inorganic hazardous air pollutant (HAP) precursors from coal. These HAP precursors are Sb, As, Be, Cd, Cr, Cl, Co, F, Pb, Hg, Mn, Ni, and Se. Although rapid physical coal cleaning is done routinely in preparation plants, biochemical processes for removal of HAP precursors from coal potentially offer advantages of deeper cleaning, more specificity, and less coal loss. Compared to chemical processes for coal cleaning, biochemical processes potentially offer lower costs and milder process conditions. Pyrite oxidizing bacteria, most notably Thiobacillusferrooxidans, are being evaluated in this project for their ability to remove HAP precursors from U.S. coals.

  19. Biochemical Removal of HAP Precursors From Coal

    International Nuclear Information System (INIS)

    Olson, G.; Tucker, L.; Richards, J.

    1997-07-01

    This project addresses DOE's interest in advanced concepts for controlling emissions of air toxics from coal-fired utility boilers. We are determining the feasibility of developing a biochemical process for the precombustion removal of substantial percentages of 13 inorganic hazardous air pollutant (HAP) precursors from coal. These HAP precursors are Sb, As, Be, Cd, Cr, Cl, Co, F, Pb, Hg, Mn, Ni, and Se. Although rapid physical coal cleaning is done routinely in preparation plants, biochemical processes for removal of HAP precursors from coal potentially offer advantages of deeper cleaning, more specificity, and less coal loss. Compared to chemical processes for coal cleaning, biochemical processes potentially offer lower costs and milder process conditions. Pyrite oxidizing bacteria, most notably Thiobacillusferrooxidans, are being evaluated in this project for their ability to remove HAP precursors from U.S. coals

  20. eQuilibrator—the biochemical thermodynamics calculator

    Science.gov (United States)

    Flamholz, Avi; Noor, Elad; Bar-Even, Arren; Milo, Ron

    2012-01-01

    The laws of thermodynamics constrain the action of biochemical systems. However, thermodynamic data on biochemical compounds can be difficult to find and is cumbersome to perform calculations with manually. Even simple thermodynamic questions like ‘how much Gibbs energy is released by ATP hydrolysis at pH 5?’ are complicated excessively by the search for accurate data. To address this problem, eQuilibrator couples a comprehensive and accurate database of thermodynamic properties of biochemical compounds and reactions with a simple and powerful online search and calculation interface. The web interface to eQuilibrator (http://equilibrator.weizmann.ac.il) enables easy calculation of Gibbs energies of compounds and reactions given arbitrary pH, ionic strength and metabolite concentrations. The eQuilibrator code is open-source and all thermodynamic source data are freely downloadable in standard formats. Here we describe the database characteristics and implementation and demonstrate its use. PMID:22064852

  1. Biochemical and serological properties of Streptococcus uberis.

    Science.gov (United States)

    Lämmler, C

    1991-12-01

    The Strep-Zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of Streptococcus uberis. The biochemical profiles of the S. uberis cultures clearly differed from those of S. agalactiae and S. dysgalactiae. Serological grouping of S. uberis revealed polysaccharide antigens of groups E, G, P and U. Some cultures of S. uberis demonstrated CAMP-like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of Helix pomatia and Dolichos biflorus. The occurrence of group polysaccharides, CAMP-like reactivities, and the lectin agglutination reactions were obviously not related to each other or to any of the biochemical properties. These reactions, possibly of importance as virulence factors, might serve as epidemiological markers.

  2. Actividad biológica e inmunológica de las isoformas de carga de la hormona luteinizante bovina

    Directory of Open Access Journals (Sweden)

    Álvaro Ortega

    2016-01-01

    Full Text Available La hormona luteinizante (LH sufre modificaciones postraduccionales que dan origen a isoformas de carga. El estudio determinó la actividad biológica (B e inmunológica (I de distintas isoformas de LH bovina. Las isoformas se aislaron mediante el cromatoenfoque a partir del extracto glicoprotéico obtenido de lóbulos anteriores de hipófisis de bovino. La actividad biológica se evaluó en un bioensayo in vitro midiendo la producción de AMPc. La actividad inmunológica se midió con un radioinmunoensayo (RIA específico para LH. El USDA-bLH-B5 se utilizó como referencia. Las isoformas se agruparon tomando como referencia el rango de pH de elución, en básicas (A, pH, 10.75-9.75; B, pH, 9.58-8.41, neutras (C, pH, 7.98-6.89 y ácidas (D, pH, 6.88-5.41; E, pH, 5.36-3.46. El peso molecular del heterodímero de cada isoforma y del estándar fue similar, estimado en 36.5 kDa. La actividad inmunológica y biológica se comportó de forma dosis-dependiente. Con respecto al estándar, se requirió una mayor concentración de proteína de cada isoforma para obtener el IC50 en la curva de inhibición. En el bioensayo, el valor EC50 para la producción de AMPc fue significativamente diferente entre isoformas; la isoforma neutra mostró un EC50 inferior lo que se interpretó como la proteína más bioactiva, en contraste, la isoforma ácida, mostró un valor de EC50 superior y resultó ser la menos bioactiva; la básica tuvo un comportamiento intermedio (P<0.05. En conclusión, los resultados sugieren un efecto diferenciado de las isoformas de carga, sobre la producción cuantitativa de AMPc por unidad de LH inmunoreactiva.

  3. Segurança do uso de terapias biológicas para o tratamento de artrite reumatoide e espondiloartrites

    Directory of Open Access Journals (Sweden)

    Licia Maria Henrique da Mota

    2015-06-01

    Full Text Available O tratamento das doenças reumáticas autoimunes sofreu uma progressiva melhora ao longo da última metade do século passado, que foi expandida com a contribuição das terapias biológicas ou imunobiológicos. No entanto, há que se atentar para as possibilidades de efeitos indesejáveis advindos da utilização dessa classe de medicações. A Sociedade Brasileira de Reumatologia (SBR elaborou um documento, baseado em ampla revisão da literatura, sobre os aspectos relativos à segurança dessa classe de fármacos, mais especificamente no que diz respeito ao tratamento da artrite reumatoide (AR e das espondiloartrites. Os temas selecionados pelos especialistas participantes, sobre os quais foram estabelecidas considerações quanto à segurança do uso de drogas biológicas, foram: ocorrência de infecções (bacterianas, virais, tuberculose, reações infusionais, reações hematológicas, neurológicas, gastrointestinais, cardiovasculares, ocorrências neoplásicas (neoplasias sólidas e da linhagem hematológica, imunogenicidade, outras ocorrências e reposta vacinal. Optou-se, por motivos didáticos, por se fazer um resumo da avaliação de segurança, de acordo com os tópicos anteriores, por classe de drogas/mecanismo de ação (antagonistas do fator de necrose tumoral, bloqueador da co-estimulação do linfócito T, depletor de linfócito B e bloqueador do receptor de interleucina-6. Em separado, foram tecidas considerações gerais sobre segurança do uso de biológicos na gravidez e na lactação. Esta revisão procura oferecer uma atualização ampla e equilibrada das experiências clínica e experimental acumuladas nas últimas duas décadas de uso de medicamentos imunobiológicos para o tratamento da AR e espondiloartrites.

  4. Nuevas orientaciones en historia económica : innovaciones biológicas y cambio técnico, siglos XIX-XX

    OpenAIRE

    Pujol Andreu, Josep

    2011-01-01

    Las innovaciones biológicas han sido siempre consustanciales al desarrollo económico, pero no suelen ser consideradas en Historia Económica. En parte esta situación es consecuencia de la escasa información disponible de aquella variable, en relación con la clase de informaciones que historiadores y economistas estamos acostumbrados a utilizar. La escasa atención de la Historia Económica por las innovaciones biológicas también está relacionada con la marcada tendencia de sus especialistas a mi...

  5. La biomonitorización de sustancias tóxicas en muestras biológicas de población general

    OpenAIRE

    Ibarluzea, Jesús; Aurrekoetxea, Juan José; Porta Serra, Miquel; Sunyer Deu, Jordi; Ballester Díez, Ferran

    2016-01-01

    La biomonitorización de sustancias tóxicas ha sido incorporada por buena parte de los países más desarrollados, con el objetivo de conocer sus concentraciones en muestras biológicas. Estas sustancias se incorporan al organismo por medio de diferentes exposiciones ambientales. La vigilancia en muestras biológicas debe permitir conocer sus valores en grupos vulnerables y su evolución en el tiempo, comparar con los valores observados en otros países, identificar grupos que presenten valores alto...

  6. Biodiscos: una alternativa de tratamiento biológico para aguas residuales cuando no se dispone de grandes extensiones de terreno

    OpenAIRE

    Deloya Martínez, Alma

    2015-01-01

    Uno de los sistemas más recientes para el tratamiento de aguas residuales es el Contador Biológico Rotatorio (CBR) comúnmente conocido como Biodisco. Este sistema de tratamiento biológico secundario es usado para la remoción de la Demanda Bioquímica de Oxígeno (DBO) y para el pulido de efluentes nitrificados1. El biodisco se usó por primera vez en Alemania en 1900 y en 1929 en los Estados Unidos. En ambos casos fueron construidos de madera. En 1950 se realizaron pruebas con discos de plástico...

  7. Disponibilidade de azoto para as plantas, a partir de fertilizantes orgânicos autorizados em agricultura biológica

    OpenAIRE

    Pereira, Anabela; Rodrigues, M.A.; Arrobas, Margarida

    2005-01-01

    Os fertilizantes orgânicos continuam a ser bastante valorizados pela agricultura. No modo de produção biológico, com as restrições ao uso de fertilizantes de síntese, os fertilizantes autorizados para aquele fim, ganham importância acrescida Neste trabalho reportam-se resultados de um ensaio que incluiu 8 tipos de fertilizantes, alguns dos quais autorizados em agricultura biológica. O trabalho está integrado numa linha de investigação mais ampla que envolve também ensaios de campo. Os fertili...

  8. Utilización de los equipos de protección individual frente al riesgo biológico por el personal sanitario

    OpenAIRE

    Angelina Constans Aubert; Rosa Mª Alonso Espadalé; Joaquín Pérez Nicolás

    2008-01-01

    El objetivo de este trabajo ha sido obtener información sobre el nivel de utilización de los equipos de protección individual (EPI) frente al riego biológico entre el personal que desarrolla su actividad profesional en centros sanitarios. Para ello se preparó un cuestionario, que previamente validado se aplicó a un colectivo de distintos centros sanitarios de Cataluña durante los años 2003/2004. La información obtenida en este estudio revela que la utilización de los EPI frente al riesgo biol...

  9. Biochemical activity of fullerenes and related derivatives

    International Nuclear Information System (INIS)

    Huczko, A.; Lange, H.; Calko, E.

    1999-01-01

    An astonishing scientific interest, embodied in over 15000 research articles so far, has been encountered since 1985 when fullerenes were discovered. From new superconductors to a rich electrochemistry and reaction chemistry, fullerene nanostructures continue to excite the scientific world, and new findings continue at record pace. This review presents many examples of the biochemical activities of fullerenes and derivatives, e. g. cytotoxic activity, selective DNA cleavage and antiviral activity against HIV. We also present some results of our testing which show that, despite its chemical and biochemical activity, fullerene matter does not present any health hazard directly related to skin irritation and allergic risks. (author)

  10. Establishment of a clonal bank of Caesalpinia spinosa (Mol. O. Kuntz by selection of plus trees and grafting

    Directory of Open Access Journals (Sweden)

    Jenny E Nuñez

    2017-03-01

    Full Text Available In Ecuador, plant propagation techniques are not available commercially to establish clonal banks of Caesalpinia spinosa (Mol. O. Kuntz (guarango plus trees, which limits the development of in vitro propagation protocols of this specie. The objective of the present work was to establish a clonal bank of C. spinosa by selecting plus trees and grafting. Guarango trees belonging to the province of Chimborazo, Guano canton were selected based on total height, height at the beginning of the crown, height of crown, crown surface, crown symmetry, flowering, fruit production and content of tannins in the pod. The patron plants to make the grafts were obtained from scarified seeds, soaked for 48 hours at room temperature and planted in beds of 1.0 x 3.0 m. At 40 days, the seedlings were transplanted into pockets and at 16 months the grafts were made in the patron plants. Three types of graft were used (simple slit in the patron, single slit in the spike and bud grafting. For each, 100 patron were used. Of the eight trees plus collected in the field, the ecotype CHSt03 was used to make the grafts. It was showed the highest total height (6.6m, height at the beginning of the cup (2.2m, cup surface (> 70%, cup volume (> 10%, cup symmetry (1, fruit (40kg / tree and total polyphenol content in pods (5870 μgEAGgMS-1. The graft by simple slit in the patron had the highest stuck percentage (80%. These results allowed to establish a clonal bank of 80 plants grafted of C. spinosa of the ecotype CHSt03, which establish the bases to develop protocols for in vitro propagation of this forest species, native of Ecuador.   Keywords: biodiversity, biotechnology, forestry, industry, tannins

  11. Aspectos biológico-pesqueros del pejerrey (Odontesthes regia regia en el puerto de Ilo durante 1996-1998

    Directory of Open Access Journals (Sweden)

    Hugo Treviño

    2014-06-01

    Full Text Available Se analizaron los aspectos biológicos y pesqueros del pejerrey peruano (Odontesthes regia regia población en Ilo, desde enero de 1996 a septiembre de 1998. Las capturas fueron tres veces más alta (72,76 t que las de 1996 hasta 1997, mientras que con el calentamiento de "El Niño" en 1998 éstas se redujeron a 0,62 t. Durante el evento "El Niño 1997-1998" la población mostró también una serie de cambios en la distribución, la reproducción y la composición de la dieta. Estos cambios se asociaron a la incursión de agua subtropical superficial.

  12. Teoría de agencia: una revisión del origen biológico del delito

    Directory of Open Access Journals (Sweden)

    Iván Araya

    2016-04-01

    Full Text Available Este trabajo aborda la causa del conflicto entre principal y agente, buscando respuestas en las disciplinas de la biología, particularmente en las ciencias de la antropología criminal. Se ha realizado un ensamble entre los hallazgos de la teoría de agencia y la teoría de los delitos de cuello blanco, reinterpretando las actuaciones del agente desde su configuración entre instinto, herencia y personalidad. Los resultados en las ciencias biológicas podrían aportar nuevas interpretaciones y explicaciones en las ciencias de la administración, avanzando hacia mejores conceptualizaciones del conflicto, mediante teorías con mayor contenido empírico. En general, pese al conflicto permanente entre las partes, su dinámica potencia los resultados perseguidos por el principal.

  13. Las ciperáceas (Cyperaceae de la Estación Biológica La Selva, Costa Rica

    Directory of Open Access Journals (Sweden)

    Jorge Gómez-Laurito

    2009-11-01

    Full Text Available La familia Cyperaceae es cosmopolita e incluye unos 115 géneros y 3600 especies. Costa Rica tiene 25 géneros y 210 especies. Aquí presento claves y descripciones para las 34 especies (10 géneros que ocurren en la Estación Biológica La Selva, Costa Rica. El género Cyperus está representado por 12 especies, seguido por Rhynchospora (6, Scleria (5, Calyptocarya (2, Eleocharis (2, Kyllinga (2, Mapania (2, Hypolytrum (1 y Lipocarpha (1. Oxycaryum cubense (Poepp & Kunth Palla, ha sido colectada en los alrededores y eventualmente podría ser encontrada en la Estación.

  14. Teoría de agencia: una revisión del origen biológico del delito

    Directory of Open Access Journals (Sweden)

    Iván Araya

    2016-01-01

    Full Text Available Este trabajo aborda la causa del conflicto entre principal y agente, buscando respuestas en las disciplinasde la biología, particularmente en las ciencias de la antropología criminal. Se ha realizado un ensambleentre los hallazgos de la teoría de agencia y la teoría de los delitos de cuello blanco, reinterpretando lasactuaciones del agente desde su configuración entre instinto, herencia y personalidad. Los resultadosen las ciencias biológicas podrían aportar nuevas interpretaciones y explicaciones en las ciencias de laadministración, avanzando hacia mejores conceptualizaciones del conflicto, mediante teorías con mayorcontenido empírico. En general, pese al conflicto permanente entre las partes, su dinámica potencia losresultados perseguidos por el principal.

  15. Búsqueda de nuevas dianas terapéuticas en psoriasis. Papel de la terapia biológica

    OpenAIRE

    Morón Romero, Rocío

    2018-01-01

    El trabajo búsqueda de nuevas dianas terapéuticas en psoriasis. Papel de la terapia biológica se centra dentro del programa biología del desarrollo, cáncer, biología molecular y farmacología. Estudiaremos las dianas terapéuticas implicadas en esta patología para decidir el tratamiento más específico a cada paciente. El descubrimiento de este tipo de terapias ha supuesto grandes avances en investigación, todavía queda mucho camino para su utilización en la práctica clínica habitual. El treb...

  16. Cirugía biológica pre protésica en artrosis temprana de rodilla

    OpenAIRE

    Wainer E. Mauricio, DR

    2014-01-01

    La artrosis (OA) de rodilla es una condición progresiva, incapacitante en distintos grados en sus etapas, terminando en el colapso y destrucción articular que requiere de cirugía protésica en las etapas más avanzadas. La cirugía biológica pre-protésica, concepto que hoy en día se implementa en el manejo de las etapas precoces de la OA, se basa en el diagnóstico precoz de esta condición y de los factores de riesgo que aseguran el progreso a estados más avanzados. Su objetivo es prolongar la vi...

  17. Nosso corpo nos pertence: a dialética do biológico e do social

    Directory of Open Access Journals (Sweden)

    Karen Mary Giffin

    1991-06-01

    Full Text Available Este artigo visa situar a discussão do biológico e do social no interior da análise da condição feminina. Com o surgimento das análises feministas, a hegemonia anterior das explicações biológicas é substituída por uma ênfase na construção social da identidade feminina. O determinismo biológico é repudiado, mesmo quando a identificação da mulher com o corpo e com a natureza e seu status secundário são considerados como universais. Neste processo de reelaboração do objeto, o papel ideológico da ciência é apontado, na medida em que a dominação masculina na ciência e na sociedade acompanhou a tendência histórica que relegou a questão da mulher à esfera natural. Embora uma crescente apreciação da construção social da atividade científica em si impulsione o abandono da dicotomia biológico/social ao nível conceitual, as diferenças entre homem e mulher na esfera da reprodução continuam a atuar. É argumentado que análise da reprodução requer caracterização dos sexos como entidades biossociais em relacionamento, situados em contextos históricos específicos, e que, na sociedade moderna, a mulher é sujeita a uma dupla contradição reprodutiva.This article aims at reviewing the discussion of biological and social factors in the analysis of women's social condition. With the appearance of a feminist perspective, the dominance of earlier biologically-based explanations was substituted by an emphasis on the social construction of female identity. Even when women's identification with the body and with nature, and their secondary status, were considered universal, biological determinism was rejected. In this process of re-definition of the object of study, the ideological role of science was pointed out, since male dominance in science and society accompanied the historical tendency which relegated "the woman question" to the sphere of natural fact. Although growing awareness of the socially-constructed nature

  18. Factores de riesgo para el desarrollo psicomotor en lactantes nacidos en óptimas condiciones biológicas

    Directory of Open Access Journals (Sweden)

    Andraca Isidora de

    1998-01-01

    Full Text Available OBJECTIVO: Evaluar el efecto de factores de riesgo para el desarrollo infantil en niños, de nivel socioeconómico bajo, nacidos en óptimas condiciones biológicas pero expuestos a condiciones sociales adversas. METODOLOGÍA: Lactantes de ambos sexos em número de 788, fueron exhaustivamente estudiados en términos de: alimentación, crecimiento, ambiente físico y psicosocial. Se controlaron prospectivamente hasta los 12 meses de edad, momento en que se evaluó el desarrollo mental (MDI y motor (PDI a través de la escala de Bayley. A partir de las variables medidas se identificó 18 factores de riesgo, los que fueron dicotomizados (bajo y alto riesgo. RESULTADOS: Lactancia materna, temperamento del niño, inteligencia materna y estimulación en el hogar se asocian consistentemente con MDI y PDI. Luego de ajustar por covariables, la estimulación en el hogar persiste como factor significativo en la explicación de la variabilidad del MDI y PDI, en tanto que el sexo del niño se consolida como factor condicionante del desarrollo motor. El efecto acumulado de 7 o más factores de riesgo se asocia con una disminución significativa de los puntajes de desarrollo. La combinación de categorías de riesgo de temperamento infantil, inteligencia materna, rol del padre y estimulación en el hogar alcanza mejor predicción del desarrollo infantil que otras combinaciones puestas a prueba en este estudio. CONCLUSIONE: Se concluye que, aún en niños biológicamente indemnes, el desarrollo psicomotor se afecta negativamente ante la presencia de condiciones ambientales adversas que actúan simultáneamente.

  19. Guerra biológica, bioterrorismo e saúde pública Biological warfare, bioterrorism and public health

    Directory of Open Access Journals (Sweden)

    Luiz Jacintho da Silva

    2001-12-01

    Full Text Available O uso de agentes biológicos como arma não é novidade para a humanidade. Durante séculos, até a atualidade, a guerra biológica tem sido objeto de muita pesquisa e especulação, mas de pouca ação. O medo de efeitos contrários e dúvidas sobre sua eficiência como armas devem ter limitado seu uso. Recentemente, se verificou uma nova forma de terrorismo, empregando agentes infecciosos, devagar e sem muito alarde, até as ocorrências recentes com o Bacillus anthracis nos Estados Unidos. A varíola é possivelmente o mais devastador desses agentes. Menos de 25 anos passados desde sua erradicação, a saúde pública tem que lidar com a possibilidade de sua re-introdução. O cenário da re-introdução da varíola no Brasil é discutido.Biological agents as weapons are not new to mankind. For centuries and into the present, biological warfare has been the subject of much research and speculation, but little action. Their limited use has probably been due to fear of unexpected counter-effects and doubts about their efficiency as weapons. Recently a new form of terrorism employing infectious agents has emerged slowly and without much fanfare, until the recent events with Bacillus anthracis in the United States. Smallpox is potentially the most devastating of these agents. Less than 25 years after the eradication of smallpox, the public health field is now forced to deal with the possibility of its re-introduction. The author discusses the scenario of smallpox re-introduction into Brazil.

  20. Factores de riesgo para el desarrollo psicomotor en lactantes nacidos en óptimas condiciones biológicas

    Directory of Open Access Journals (Sweden)

    Isidora de Andraca

    1998-04-01

    Full Text Available OBJECTIVO: Evaluar el efecto de factores de riesgo para el desarrollo infantil en niños, de nivel socioeconómico bajo, nacidos en óptimas condiciones biológicas pero expuestos a condiciones sociales adversas. METODOLOGÍA: Lactantes de ambos sexos em número de 788, fueron exhaustivamente estudiados en términos de: alimentación, crecimiento, ambiente físico y psicosocial. Se controlaron prospectivamente hasta los 12 meses de edad, momento en que se evaluó el desarrollo mental (MDI y motor (PDI a través de la escala de Bayley. A partir de las variables medidas se identificó 18 factores de riesgo, los que fueron dicotomizados (bajo y alto riesgo. RESULTADOS: Lactancia materna, temperamento del niño, inteligencia materna y estimulación en el hogar se asocian consistentemente con MDI y PDI. Luego de ajustar por covariables, la estimulación en el hogar persiste como factor significativo en la explicación de la variabilidad del MDI y PDI, en tanto que el sexo del niño se consolida como factor condicionante del desarrollo motor. El efecto acumulado de 7 o más factores de riesgo se asocia con una disminución significativa de los puntajes de desarrollo. La combinación de categorías de riesgo de temperamento infantil, inteligencia materna, rol del padre y estimulación en el hogar alcanza mejor predicción del desarrollo infantil que otras combinaciones puestas a prueba en este estudio. CONCLUSIONE: Se concluye que, aún en niños biológicamente indemnes, el desarrollo psicomotor se afecta negativamente ante la presencia de condiciones ambientales adversas que actúan simultáneamente.

  1. Recubrimientos de pisos en áreas controladas de la producción de biológicos

    Directory of Open Access Journals (Sweden)

    Pedro A. Rodríguez

    2007-12-01

    Full Text Available El recubrimiento de los pisos, utilizando las resinas sintéticas autonivelantes en la construcción de las áreas controladas, empleadas en las instalaciones de producción de biológicos, en las dedicadas a los procesos de manipulación de microorganismos (contención, así como en las instalaciones para los animales de laboratorio y procesos asépticos de formulación farmacéutica, contribuyen a la seguridad biológica y farmacéutica, según sea el caso, por su continuidad integral y garantía de la calidad microbiológica del aire, así como al ahorro energético, pues disminuyen la ganancia de calor latente atribuido a la humedad incorporada al aire por otros recubrimientos permeables. Las condiciones climáticas de Cuba y la dureza del agua empleada en las mezclas cementosas son un alto potencial para la formación de "burbujas", tema abordado en este trabajo. El conocimiento de los aspectos que provocan la formación de estas ampollas y las vías para evitarlo, son útiles y de un valor apreciable. En este artículo se presentan las experiencias obtenidas con la aplicación de los recubrimientos epoxídicos en las áreas controladas del Instituto Finlay, instalaciones que no han presentado deformaciones apreciables después de 15 años de explotación.

  2. Cirugía biológica pre protésica en artrosis temprana de rodilla

    Directory of Open Access Journals (Sweden)

    Wainer E. Mauricio, DR

    2014-09-01

    Full Text Available La artrosis (OA de rodilla es una condición progresiva, incapacitante en distintos grados en sus etapas, terminando en el colapso y destrucción articular que requiere de cirugía protésica en las etapas más avanzadas. La cirugía biológica pre-protésica, concepto que hoy en día se implementa en el manejo de las etapas precoces de la OA, se basa en el diagnóstico precoz de esta condición y de los factores de riesgo que aseguran el progreso a estados más avanzados. Su objetivo es prolongar la vida útil de la articulación nativa y retardar o eliminar la necesidad de una prótesis de rodilla. Esto ha motivado innumerables estudios preclínicos y clínicos en base al uso de nuevos elementos de ingeniería de tejidos, uso de implantes de condrocitos autólogos y manejo de células madre. Los resultados en el corto y mediano plazo son auspiciosos, pero se requieren mejores niveles de evidencia científica de estos estudios y seguimientos más prolongados. En el presente artículo se analiza el concepto de la artrosis temprana, la detección de las lesiones de cartílago y los factores de riesgo de los pacientes que los llevan a una artrosis y los protocolos actuales de tratamiento biológico de Clínica Las Condes.

  3. Acidentes com material biológico relacionados ao trabalho: análise de uma abordagem institucional

    Directory of Open Access Journals (Sweden)

    Ana Isabel Dias da Silva

    Full Text Available RESUMO OBJETIVO: analisar o perfil de relatórios, instrumentos de registros e fluxos de tratamento, notificações, acompanhamentos, profilaxias e encaminhamentos devidos a acidentes causados por material perfurocortante em trabalhadores de saúde em uma instituição de saúde pública. Métodos: a coleta de dados usou um questionário semiestruturado aplicado aos profissionais responsáveis pelos registros de acidentes e avaliou os formulários utilizados em três setores da instituição de estudo. RESULTADOS: foram analisados 134 registros de acidentes biológicos. Quando comparamos os registros da instituição com os 50 registrados no mesmo período pelo banco de dados da Secretaria Municipal de Saúde do Rio de Janeiro, apenas 11 casos foram concordantes. Também identificamos falta de padronização nos formulários utilizados, fluxos diferentes e falta de registros de investigações dos casos, além de ausência de acompanhamento do desfecho dos acidentes. CONCLUSÃO: constataram-se subnotificação e discordância entre as fontes. Um formulário de registro para acidentes biológicos foi elaborado para informações de notificação e investigações, bem como criado um programa para monitorar acidentes causados por material perfurocortante, visando melhorar a vigilância de saúde dos trabalhadores da instituição.

  4. Haematological And Biochemical Effects Of Sulphadimidine In ...

    African Journals Online (AJOL)

    Haematological and biochemical efects of sulphadmidine were studied in Nigerian mongrel dogs. Five Nigerian mongrel dogs of either sex weighing between 7 and 12 kg were used for the study. The pretreatment blood and serum samples were collected and the weight of animals taken before the administraton of 100 ...

  5. Haematological and biochemical responses of starter broiler ...

    African Journals Online (AJOL)

    A study was conducted to investigate the haematological and biochemical responses of starter broiler chickens fed copper and probiotics supplemented diets. A total of 180-day old Marshal broiler chicks were randomly allotted to six treatment groups of 30 birds each. The treatments were divided into three replicates of ten ...

  6. Estimation of biochemical variables using quantumbehaved particle ...

    African Journals Online (AJOL)

    Due to the difficulties in the measurement of biochemical variables in fermentation process, softsensing model based on radius basis function neural network had been established for estimating the variables. To generate a more efficient neural network estimator, we employed the previously proposed quantum-behaved ...

  7. Biochemical Markers of Joint Tissue Turnover

    DEFF Research Database (Denmark)

    Bay-Jensen, Anne-Christine; Sondergaard, Bodil Cecilie; Christiansen, Claus

    2009-01-01

    Recent disappointments in late stage developments of anti-osteoarthritic drugs have reinforced efforts to develop better biomarkers for application in both the drug development process as well as in the routine management of these patients. Here we provide a brief review of biochemical tests...

  8. EDITORIAL A CASCADE OF BIOCHEMICAL AND PHYSIOLOGICAL ...

    African Journals Online (AJOL)

    pharm-chem

    A CASCADE OF BIOCHEMICAL AND PHYSIOLOGICAL MARKERS IN PATHOLOGICAL. DISORDERS. A few years ago, a neurophysiology lecturer in the Department of Medical Physiology, University of. Nairobi, was doing ... from haemolysis of RBC is rapidly distributed into cells and excreted in kidney. It would have been.

  9. Isolation, molecular and biochemical characterization of oil ...

    African Journals Online (AJOL)

    Biochemical and physiological characterization performed on the 34 bacterial isolates, revealed the presence of oil biodegrading bacterial genera and species of Pseudomonas Acidovorans, P. aerugi-nosa, P. alcaligenes, P. fluorescens, P. cepacia, P. mallei, P. maltophilia, P. oleovorans, P. putida, P. stutzeri P. vesicularis, ...

  10. Some haematological, biochemical and zootechnical parameters of ...

    African Journals Online (AJOL)

    biochemical and zootechnical parameters of a colony of fruit eating bats (Eidolon helvum) in Morogoro region, Tanzania. A total of 50 bats were captured using hand held hooks and quickly transported to the laboratory where the various parameters were measured. Zootechnical parameters (body length, wing span and ...

  11. Biochemical Applications in the Analytical Chemistry Lab

    Science.gov (United States)

    Strong, Cynthia; Ruttencutter, Jeffrey

    2004-01-01

    An HPLC and a UV-visible spectrophotometer are identified as instruments that helps to incorporate more biologically-relevant experiments into the course, in order to increase the students understanding of selected biochemistry topics and enhances their ability to apply an analytical approach to biochemical problems. The experiment teaches…

  12. Reference Physiological Ranges for Serum Biochemical Parameters ...

    African Journals Online (AJOL)

    clinical trials and scaling up of ARV drugs among AIDS patients. ... parametric and non parametric statistics for analyses with 2.5 and 97.5 percentiles considered ..... Physiological Ranges for Plasma Biochemical Parameters among Adult Healthy Cameroonians. Urban. Rural. Males. Females. Males. Females. Parameter.

  13. Biochemical and Kinetic Characterization of Geranylgeraniol 18 ...

    African Journals Online (AJOL)

    This enzyme and its gene are an attractive target for development of plaunotol production and its detailed biochemical properties need to be understood. Recently, even though the gene (CYP97C27) coding for GGOH 18-hydroxylase has been identified, cloned, and expressed in Escherichia coli system, the enzyme activity ...

  14. Characterizing multistationarity regimes in biochemical reaction networks.

    Directory of Open Access Journals (Sweden)

    Irene Otero-Muras

    Full Text Available Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space.

  15. Probabilistic sensitivity analysis of biochemical reaction systems.

    Science.gov (United States)

    Zhang, Hong-Xuan; Dempsey, William P; Goutsias, John

    2009-09-07

    Sensitivity analysis is an indispensable tool for studying the robustness and fragility properties of biochemical reaction systems as well as for designing optimal approaches for selective perturbation and intervention. Deterministic sensitivity analysis techniques, using derivatives of the system response, have been extensively used in the literature. However, these techniques suffer from several drawbacks, which must be carefully considered before using them in problems of systems biology. We develop here a probabilistic approach to sensitivity analysis of biochemical reaction systems. The proposed technique employs a biophysically derived model for parameter fluctuations and, by using a recently suggested variance-based approach to sensitivity analysis [Saltelli et al., Chem. Rev. (Washington, D.C.) 105, 2811 (2005)], it leads to a powerful sensitivity analysis methodology for biochemical reaction systems. The approach presented in this paper addresses many problems associated with derivative-based sensitivity analysis techniques. Most importantly, it produces thermodynamically consistent sensitivity analysis results, can easily accommodate appreciable parameter variations, and allows for systematic investigation of high-order interaction effects. By employing a computational model of the mitogen-activated protein kinase signaling cascade, we demonstrate that our approach is well suited for sensitivity analysis of biochemical reaction systems and can produce a wealth of information about the sensitivity properties of such systems. The price to be paid, however, is a substantial increase in computational complexity over derivative-based techniques, which must be effectively addressed in order to make the proposed approach to sensitivity analysis more practical.

  16. Histopathological and biochemical disrupting effects of Escravos ...

    African Journals Online (AJOL)

    user

    2014-02-27

    Feb 27, 2014 ... The aim of this study was to investigate the histological and biochemical disrupting effects of Escravos ... rupturing or leaking of production infrastructures that are described as, “very old and lack regular ... crude oil were measured in weight on an electronic weighing balance and given orally (oral gavage) ...

  17. Structural and biochemical characterization of autotaxin

    NARCIS (Netherlands)

    Hausmann, Jens

    2013-01-01

    Autotaxin (ATX) was originally discovered as an “autocrine motility factor” from melanoma cells, more than two decades ago, but its biochemical function remained elusive. It took another decade to show that ATX functions as a lysophospholipase D that generates the lipid growth factor

  18. Biochemical and serological characterization of Escherichia coli ...

    African Journals Online (AJOL)

    This study was designed to determine the isolation rate, serotypes and biochemical profiles of E. coli from colibacillosis and dead-in-shell embryos in Zaria, Northern-Nigeria. The isolation rate of E. coli from hatcheries studied were 4.67% and 7.50% from farms of Simtu Agricultural Company and National Animal Production ...

  19. Biochemical and secondary metabolites changes under moisture ...

    African Journals Online (AJOL)

    The study showed the importance of carbohydrate and nitrogen cycle related metabolites in mediating tolerance in cassava by affecting their phenotypic expression in the plant. Keywords: Hydrothermal stress, bio-chemicals, pigments, secondary metabolites, cassava. African Journal of Biotechnology, Vol 13(31) 3173-3186 ...

  20. Growth, haematological and biochemical responses of growing ...

    African Journals Online (AJOL)

    Growth, haematological and biochemical responses of growing lambs injected. A.N.M Nour El-Din, S.Z El-Zarkouny, S.Z El-Zarkouny, H Ghobashy, H Ghobashy, E.I Abdel-Gawad, E.I Abdel-Gawad, D.J Kesler, D.J Kesler ...

  1. Reference Physiological Ranges for Serum Biochemical Parameters ...

    African Journals Online (AJOL)

    Background: A valid scientific evaluation of the efficacy of HIV vaccines or antiretroviral drugs includes measurement of changes in physiological parameters of subjects from known established baseline reference ranges. This study was designed to establish reference ranges for biochemical parameters among healthy ...

  2. Biochemical analysis of Brachystegia aurycoma harms seeds ...

    African Journals Online (AJOL)

    A biochemical analysis of Brachystegia aurycoma harm seeds was conducted. Brachystegia, of the family Leguminosae sub-family Caesalpinioidae is a timber tree whose seeds “achi”, are relished as soup condiment by the lgbo-speaking people of Nigeria. The seed were subjected to oil extraction using Soxhlet method.

  3. Haematological and Biochemical Studies on some Ruminants ...

    African Journals Online (AJOL)

    Michael Horsfall

    CHUKU, L C; *UWAKWE, A A. Department of Biochemistry, University of Port Harcourt, Rivers State, Nigeria. ABSTRACT: Haematological studies of ruminant vertebrates (cow, sheep and goat) present one acceptable method of understanding the ecological biochemical and physiological relationship between lower and ...

  4. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and basophiles counts were ...

  5. Some hematological and biochemical parameters in smokeless ...

    African Journals Online (AJOL)

    AJB SERVER

    2007-01-04

    Jan 4, 2007 ... The effect of Jharda powder (smokeless tobacco) on some hematological and biochemical parameters in consumers was investigated. Hematological parameters including hemoglobin content and white blood cell and leukocyte counts were higher in jharda powder consumers, while monocytes and.

  6. Biochemical reference values in elderly black subjects

    African Journals Online (AJOL)

    1990-09-01

    Sep 1, 1990 ... the resulting estimate of normal range. Clin Chem 1971; 17: 275-284. 13. Wooton IDO, King E]. Normal values for blood constituents: inter-hospital differences. Lancer 1953; I: 70. 14. Chen FWK, Millard PH. The effect of ageing on certain biochemical values. Mod Geriarrics 1972; 2: 92. IS. Forbes GB, Reina ...

  7. Preliminary biochemical and haematological effects of aqueous ...

    African Journals Online (AJOL)

    Some biochemical and haematological parameters were assayed in rats fed aqueous pulp suspension of hyphaene thebaica (L) mart. Sixteen white albino rats of the wistar strain weighing between 90-110g were grouped into four groups of four rats each. Group 1 served as control while groups 2, 3 and 4 were given ...

  8. Biochemical applications of FT-IR spectroscopy

    NARCIS (Netherlands)

    Pistorius, A.M.A.

    1996-01-01

    This thesis describes the use of (FT-)IR spectroscopy in general biochemical research. In chapter 3, IR spectroscopy is used in the quantitation of residual detergent after reconstitution of an integral membrane protein in a pre-defined lipid matrix. This chapter discusses the choice of the

  9. Histopathological and biochemical disrupting effects of Escravos ...

    African Journals Online (AJOL)

    The histological findings include: lymphocytic infiltration, cirrhosis, fibrosis, hemosiderin, oedema, mild tissue scaring and tissue necrosis. Thus, this result suggests that Escravos crude oil is a potential biochemical disruptor and can also affect the micro-architecture of liver and heart. Key words: Escravos crude oil, liver, ...

  10. Selected plasma biochemical parameters in improved indigenous ...

    African Journals Online (AJOL)

    This study was to assess the biochemical parameters of apparently healthy NIGERHYB pigs and comparison of age and sex related differences in these parameters. One hundred and thirty five NIGERHYB pigs (35 boar, 35 sow, 30 weaned boar piglets and 35 weaned gilt piglets) obtained from intensively managed pig ...

  11. Discordant results between biochemical and molecular transthyretin

    Indian Academy of Sciences (India)

    Discordant results between biochemical and molecular transthyretin assays: lessons learned from a unique testing algorithm at the Mayo Clinic. Honey V. Reddi Brittany C. Thomas Kurt S. Willkomm Matthew J. Ferber Kandelaria M. Rumilla Kimiyo M. Raymond John F. O'Brien W. Edward Highsmith. Research Note Volume ...

  12. 2009 Biochemical Conversion Platform Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Ferrell, John [Office of Energy Efficiency and Renewable Energy (EERE), Washington, DC (United States)

    2009-12-01

    This document summarizes the recommendations and evaluations provided by an independent external panel of experts at the U.S. Department of Energy Biomass Program’s Biochemical Conversion platform review meeting, held on April 14-16, 2009, at the Sheraton Denver Downtown, Denver, Colorado.

  13. Characterization of biochemical behavior of sorghum ( Sorghum ...

    African Journals Online (AJOL)

    The aim of this research was to characterize the biochemical behavior of sorghum plants under saline stress using multivariate statistical analysis methods for efficient management of Sorghum bicolor [Moench.]). The experimental design was completely randomized design composed of three saline concentrations (0, 1.5 ...

  14. Drinking patterns: biochemical and haematological findings in ...

    African Journals Online (AJOL)

    Administrator

    drinking patterns on biochemical and haematological parameters in alcohol consumers in Ile-Ife. Conigrave et al. (1995) stated that a variety of blood tests have been used to aid the assessment of drinking history and that more recently, laboratory tests based on urine, breath and sweat analyses have been investigated.

  15. Biochemical characterization of sea buckthorn ( Hippophae ...

    African Journals Online (AJOL)

    Sea buckthorn is a valuable medicinal plant, cultivated and naturally grown in northern Pakistan. The plant produces berry with small hard seed in the centre. The seed is the source of all nutrients and phytochemcials. However, there is lack of literature regarding the biochemical and physico-chemical quality of the seed.

  16. Effects Of Ceftriaxone On Haematological And Biochemical ...

    African Journals Online (AJOL)

    Short-term effects of ceftriaxone on haematological and biochemical parameters of Nigerian local turkey poults were studied. The pre-treatment blood and serum samples were collected and the weight of animals taken before the administration of body weight for a period of 4 days. The animals were weighed daiy.

  17. Growth performance, hematological and serum biochemical ...

    African Journals Online (AJOL)

    A twelve (12) weeks feeding trial was carried out to determine the effect of varying dietary inclusions of Microdesmis puberula leaf meal (MPLM) on the growth performance, hematological indices and serum biochemical constituents of growing rabbits. Four grower rabbit diets were formulated to contain the leaf meal at ...

  18. Evaluation of Haematological and Biochemical Parameters of ...

    African Journals Online (AJOL)

    ADOWIE PERE

    Evaluation of Haematological and Biochemical Parameters of Juvenile Oreochromis niloticus after Exposure to Water Soluble Fractions of ... niloticus were evaluated. After a preliminary determination of the 96 h-LC50 of ... evaporation, dissolution, emulsion, photolysis and biodegradation which generate a water soluble.

  19. Biochemical and microstructural characteristics of meat samples ...

    African Journals Online (AJOL)

    This study was conducted to compare the efficiency of different plant proteases for changing biochemical and microstructural characteristics in muscle foods. The meat samples from chicken, giant catfish, pork and beef were treated with four types of proteolytic enzymes: Calotropis procera latex proteases, papaya latex ...

  20. Dopant occupancy and UV-VIS-NIR spectroscopy of Mg (0, 4, 5 and 6 mol.%):Dy:LiNbO3 crystal

    Science.gov (United States)

    Dai, Li; Liu, Chunrui; Han, Xianbo; Wang, Luping; Tan, Chao; Yan, Zhehua; Xu, Yuheng

    2017-09-01

    A series of Dy:LiNbO3 crystals with x mol.% Mg2+ ions (x =0, 4, 5 and 6 mol.%) were grown by the Czochralski method. The effective segregation coefficient of Mg2+ and Dy3+ ions was studied by the inductively coupled plasma-atomic emission spectrometry (ICP-AES). UV-VIS-NIR absorption spectra and Judd-Ofelt theory were used to investigate their spectroscopic properties. J-O intensity parameters (Ω2 = 7.53 × 10-20cm2, Ω4 = 6.98 × 10-20cm2, and Ω6 = 3.09 × 10-20cm2) and larger spectroscopic quality factor (X = 2.26) for Mg:(6 mol.%)Dy:LiNbO3 crystals were obtained.

  1. Resposta biológica de ratos alimentados com resíduo da fermentação de batata (Solanumtuberosum L. Biological response of rats fed with the fermentation of potato residue (Solanumtuberosum L.

    Directory of Open Access Journals (Sweden)

    Luciana Dapieve Patias

    2011-07-01

    Full Text Available O presente trabalho teve por objetivo determinar a composição centesimal do tubérculo de batata in natura a ser utilizado no processo de destilação para a produção de etanol e do resíduo sólido resultante da fermentação deste tubérculo. Com base na composição do resíduo, avaliou-se o efeito da sua utilização na substituição de diferentes teores de fibra alimentar de uma dieta padrão, na resposta biológica de ratos. Para a avaliação dos efeitos biológicos, foram utilizados 32 ratos Wistar divididos em quatro grupos. Um dos grupos de animais recebeu a dieta padrão para ratos em crescimento, e os demais grupos receberam esta dieta com substituição de 25, 50 ou 100% de sua fibra alimentar pela fibra do resíduo. Durante o período experimental (23 dias, foram avaliados o consumo, o ganho de peso, o coeficiente de eficiência alimentar (CEA, a produção de fezes secas e úmidas, pH e excreção de nitrogênio fecal. No final do ensaio, foram avaliados o peso do fígado e da gordura epididimal, níveis sanguíneos de triglicerídeos, colesterol total, colesterol HDL e proteínas totais. O resíduo apresentou uma composição centesimal de 8,2% de proteína, 16,7% de fibra alimentar, 62,8% de carboidratos e 288Kcal. Os resultados obtidos no ensaio biológico sugerem que o resíduo sólido da fermentação de tubérculos de batata pode ser empregado como fonte de nutrientes, com base na substituição da fração fibra de uma dieta padrão AIN-93, sem comprometer o desenvolvimento dos ratos e os parâmetros bioquímicos analisados.The present research had the objectives of determining the in natura potato tuber centesimal composition in the distilling process in order to produce ethanol and to verify the solid residues resulting from this tuber fermentation process. Based on the residue composition, it was evaluated the effect of its use in the substitution of different dietary fiber content of a standard diet observing the

  2. Keramzito gamyba naudojant nesipučiantį molį, sapropelį ir glicerolį

    Directory of Open Access Journals (Sweden)

    Giedrius VAICKELIONIS

    2011-09-01

    Full Text Available Šio darbo tikslas - ištirti keraminių plytų ir čerpių gamybai netinkančio, silpnai pučiančiosi (išsipūtimo koeficientas Kp = 2 - 2,5 ir nesipučiančio (Kp < 2 molio panaudojimo keramzito gamybai galimybes, molio pūtimuisi gerinti dedant šių organinių priedų: biodyzelino gamybos atliekos - glicerolio, medienos drožlių plokščių pjuvenų ir organinio sapropelio. Keramzito izoliacinės savybės gerėja didėjant išdegto molio šukės akytumui. Kad molis pūstųsi geriau, į jį dažnai įmaišoma organinių priedų. Tirtas Krūnos telkinio III sluoksnio karbonatingasis molis be priedų netinka keramzito gamybai dėl per mažo išsipūtimo koeficiento (Kp = 1,25. Tam tikslui į išdžiovintą smulkiai maltą molį buvo įmaišoma įvairūs kiekiai (0 %, 1 %, 2 %, 3 %, 5 %, 7 % ir 10 % organinių atliekų. Granulės gamintos su vienu arba keliais pasirinktais degimo metu dujas išskiriančiais priedais. Suformuotos ir išdžiovintos granulės degtos skirtingose temperatūrose iki jų apsilydimo temperatūros - nuo 1090 °C iki 1170 °C. Nustatytas išdegtų granulių pūtimasis ir vandens įmirkis. Tyrimų metu nustatyta, kad organinės atliekos yra efektyvus priedas, didinantis molio struktūros akytumą. Be to, molyje neturi būti daugiau kaip 5 % sapropelio, rekomenduojamas glicerolio kiekis yra 1 % - 3 %, optimalus pjuvenų kiekis - 3 %. Bandinių, pagamintų su nurodytais priedų kiekiais ir išdegtų skirtingose temperatūrose, vandens įmirkis neviršija 15 %.http://dx.doi.org/10.5755/j01.ms.17.3.600

  3. Geochemical characterisation of kerogen from the Boom Clay Formation (Mol, Belgium) and evolution under different thermal stress

    International Nuclear Information System (INIS)

    Deniau, I.

    2002-12-01

    The Boom clay formation in Belgium has been chosen as test site for the disposal of high level radioactive wastes. The organic matter present in the clay (kerogen) is sensible to the thermal stress and can generate a huge number of gaseous and liquid compounds leading to local pH changes and to fracturing processes. In particular, some polar compounds can complex radionuclides. The samples analyzed in this work were taken in the underground laboratory of Mol at a 223 m depth. They have been analyzed in detail using geochemical methods (Rock-Eval pyrolysis, element analysis, transmission and scanning electron microscopy), spectroscopic methods (Fourier transformation infrared spectroscopy, solid state 13 C NMR, Raman) and pyrolytic methods (off-line, on-line and in sealed tubes combined with coupled CG/SM analyses). The study of a representative sample of this formation has permitted to characterize the organic matter at the molecular scale, to determine its fossilization mechanisms and the nature of the organic compounds trapped inside the kerogen. The organic matter of the Boom clays comes mainly from phyto-planktonic matter with an important contribution of terrestrial and bacterial matter. The degradation-recondensation played an important role in its preservation but the presence of numerous oxygenated molecules implies that oxidative incorporation also participated to this preservation. Finally, various products (hydrocarbons, oxygenated and nitrogenous polar compounds) trapped in significant amount inside the macro-molecular structure are released under a relatively weak thermal stress. Moreover several small polar organic molecules are released and can play a significant role in the retention or migration of radionuclides inside the geologic barrier. A sample submitted to a in-situ thermal stress of 80 deg. C during 5 years (Cerberus experiment) do not show any significant change in its kerogen structure with respect to the non-heated reference sample

  4. ¿Y si estábamos empezando la casa por el tejado?: Los genes y la caracterización (biológica del lenguaje

    Directory of Open Access Journals (Sweden)

    Antonio Benítez-Burraco

    2010-10-01

    Full Text Available Las evidencias de carácter biológico sugieren que, si bien la facultad del lenguaje puede considerarse, en lo sustancial, una capacidad cognitiva funcionalmente independiente, no lo sería por completo en términos genéticos/moleculares, neuronales o evolutivos.

  5. Comportamento parental de Brachyteles hypoxanthus (Kuhl 1820) (Primates - Atelidae) na estação biológica de Caratinga - MG /

    OpenAIRE

    Souza, Karynna Tolentino de

    2007-01-01

    Orientador : Fernando C. Passos Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Programa de Pós-Graduação em Zoologia. Defesa: Curitiba, 2007 Inclui bibliografia e anexos Area de concentração: Zoologia

  6. Efecto de la caminata y el Tai Chi sobre los marcadores biológicos de estrés oxidativo e inflamación crónica en ancianos /

    OpenAIRE

    Rosado Pérez, Juana

    2011-01-01

     tesis que para obtener el grado de Doctor en Ciencias Biológicas, presenta Juana Rosado Pérez ; asesor Víctor Manuel Mendoza Núñez. V, 152 páginas : diagramas, fotografías. Doctorado en Ciencias Biológicas UNAM, Facultad de Estudios Superiores Zaragoza, 2011

  7. Percepción del riesgo biológico en dos entidades de ciencia del sector salud en Holguín: cuba

    Directory of Open Access Journals (Sweden)

    Dailín Cobos Valdes

    Full Text Available Introducción: La temática sobre la percepción de riesgos resulta de gran actualidad e importancia porque su interpretación conduce al enfrentamiento de comportamientos humanos diversos. La misma puede ser aplicada a problemas de mayor especialización como el riesgo tecnológico o biológico. Las entidades con riesgo biológico deben conocer la percepción de sus trabajadores para poder organizar la Seguridad Biológica y proteger al trabajador ocupacionalmente expuesto. Las dos entidades de ciencia con riesgo biológico del sector salud en Holguín no presentan esta determinación. Objetivo: Determinar la percepción de riesgo biológico en estas entidades. Materiales y Métodos: Para determinar la percepción del riesgo biológico, se empleó el programa RISKPERCEP, se utilizó también el método Delphi para validar la encuesta elaborada ajustada al grupo de estudio. Resultados: La percepción del riesgo en las dos entidades fue baja. Este resultado tiene notables implicaciones en la Bioseguridad, ya que al no valorar el riesgo al que están expuestos, los trabajadores, se familiarizan con las actividades con riesgo biológico que ejecutan diariamente y son proclives a cometer fallas. En sentido general, en las dos entidades, las variables a nivel de individuos y de la naturaleza del riesgo se encuentran subestimadas y las de gestión se encuentran sobrestimadas. Conclusiones: Los estudios realizados permiten determinar sobre qué aspectos debe incidirse para regular la sobrestimación o subestimación del riesgo, los que son esenciales en el diseño de políticas adecuadas de capacitación y en la preparación de mensajes de prevención en el ámbito laboral.

  8. Kombucha tea fermentation: Microbial and biochemical dynamics.

    Science.gov (United States)

    Chakravorty, Somnath; Bhattacharya, Semantee; Chatzinotas, Antonis; Chakraborty, Writachit; Bhattacharya, Debanjana; Gachhui, Ratan

    2016-03-02

    Kombucha tea, a non-alcoholic beverage, is acquiring significant interest due to its claimed beneficial properties. The microbial community of Kombucha tea consists of bacteria and yeast which thrive in two mutually non-exclusive compartments: the soup or the beverage and the biofilm floating on it. The microbial community and the biochemical properties of the beverage have so far mostly been described in separate studies. This, however, may prevent understanding the causal links between the microbial communities and the beneficial properties of Kombucha tea. Moreover, an extensive study into the microbial and biochemical dynamics has also been missing. In this study, we thus explored the structure and dynamics of the microbial community along with the biochemical properties of Kombucha tea at different time points up to 21 days of fermentation. We hypothesized that several biochemical properties will change during the course of fermentation along with the shifts in the yeast and bacterial communities. The yeast community of the biofilm did not show much variation over time and was dominated by Candida sp. (73.5-83%). The soup however, showed a significant shift in dominance from Candida sp. to Lachancea sp. on the 7th day of fermentation. This is the first report showing Candida as the most dominating yeast genus during Kombucha fermentation. Komagateibacter was identified as the single largest bacterial genus present in both the biofilm and the soup (~50%). The bacterial diversity was higher in the soup than in the biofilm with a peak on the seventh day of fermentation. The biochemical properties changed with the progression of the fermentation, i.e., beneficial properties of the beverage such as the radical scavenging ability increased significantly with a maximum increase at day 7. We further observed a significantly higher D-saccharic acid-1,4-lactone content and caffeine degradation property compared to previously described Kombucha tea fermentations. Our

  9. Management Options for Biochemically Recurrent Prostate Cancer.

    Science.gov (United States)

    Fakhrejahani, Farhad; Madan, Ravi A; Dahut, William L

    2017-05-01

    Prostate cancer is the most common solid tumor malignancy in men worldwide. Treatment with surgery and radiation can be curative in organ-confined disease. Unfortunately, about one third of men develop biochemically recurrent disease based only on rising prostate-specific antigen (PSA) in the absence of visible disease on conventional imaging. For these patients with biochemical recurrent prostate cancer, there is no uniform guideline for subsequent management. Based on available data, it seems prudent that biochemical recurrent prostate cancer should initially be evaluated for salvage radiation or prostatectomy, with curative intent. In selected cases, high-intensity focused ultrasound and cryotherapy may be considered in patients that meet very narrow criteria as defined by non-randomized trials. If salvage options are not practical or unsuccessful, androgen deprivation therapy (ADT) is a standard option for disease control. While some patients prefer ADT to manage the disease immediately, others defer treatment because of the associated toxicity. In the absence of definitive randomized data, patients may be followed using PSA doubling time as a trigger to initiate ADT. Based on retrospective data, a PSA doubling time of less than 3-6 months has been associated with near-term development of metastasis and thus could be used signal to initiate ADT. Once treatment is begun, patients and their providers can choose between an intermittent and continuous ADT strategy. The intermittent approach may limit side effects but in patients with metastatic disease studies could not exclude a 20% greater risk of death. In men with biochemical recurrence, large studies have shown that intermittent therapy is non-inferior to continuous therapy, thus making this a reasonable option. Since biochemically recurrent prostate cancer is defined by technological limitations of radiographic detection, as new imaging (i.e., PSMA) strategies are developed, it may alter how the disease is

  10. Depuración de las aguas de lavado de aceitunas negras mediante procesos biológico aerobio y de ozonización

    Directory of Open Access Journals (Sweden)

    García Rodríguez, Juan

    2001-08-01

    Full Text Available The purification of black olive washed waters has been studied by an aerobic biological treatment, an ozonation process and by the combination of both sequential processes: ozone-biological and biological-ozone. Biodegradation experiments have been carried out modifying the initial substrate and biomass concentration. In the ozonation experiments, temperature, pH and ozone partial pressure has been varied. A kinetic study applying the Contois’model to the biological experimental data was performed. In the ozonation process, supposing a firsts-order kinetic reaction with respect to COD and ozone, the kinetic constant was determined. In both combined treatments, the previous stage improved the reaction rate of the second stage. For the purification of these wastewaters, an aerobic biological treatment followed by an ozonation process was the most effective sequence.Se ha estudiado la depuración de las aguas de lavado del procesado de aceitunas negras por tratamientos biológico aerobio y de ozonización y por procesos combinados secuenciales ozono-biológico y biológico-ozono. Se han llevado a cabo experimentos de biodegradación modificando las concentraciones iniciales de sustrato y de biomasa. En los experimentos de ozonización se ha variado la temperatura, el pH y la presión parcial de ozono. Se ha realizado un estudio cinético aplicando el modelo de Contois a los procesos biológicos. Considerando reacciones de primer orden respecto de la DQO, se ha determinado la constante de velocidad en los procesos de ozonización. En los tratamientos combinados, la etapa previa supuso una mejora en la constante de velocidad de la segunda etapa. La secuencia depuración biológica aerobia seguida de ozonización resultó el proceso más eficaz para el tratamiento de estas aguas.

  11. Perfil epidemiológico de acidentes com material biológico entre estudantes de medicina em um pronto-socorro cirúrgico

    Directory of Open Access Journals (Sweden)

    Phillipe Geraldo Teixeira de Abreu Reis

    Full Text Available OBJETIVO: avaliar os acidentes com material biológico entre estudantes de medicina estagiando em um pronto-socorro de trauma e identificar as principais situações relacionadas, causas atribuídas e prevenção. MÉTODOS: estudo com abordagem quantitativa. Os dados foram coletados através de um questionário, aplicado via internet, contendo perguntas fechadas de escolha múltipla, referentes a acidentes com material biológico. A amostra obtida foi 100 estudantes. RESULTADOS: trinta e dois se acidentaram com materiais biológicos. As atividades de maior risco foram anestesia local (39,47%, sutura (18,42% e recapeamento de agulha (15,79%. As principais vias de exposição ao material biológico foram contato com olho ou mucosa, com 34%, através de seringa com agulha com 45%. Após a contaminação, apenas 52% notificaram o acidente ao setor responsável. CONCLUSÃO: as principais causas de acidente encontradas e vias de exposição podem ser atribuídas a diversos fatores, como falta de treinamento e ao não uso de equipamentos de proteção individual. Ações preventivas e educativas são de extrema importância para diminuir a incidência dos acidentes com materiais biológicos e melhorar a conduta pós-exposição. É preciso entender as principais causas atribuídas e situações relacionadas a fim de implantar medidas gerais e eficazes.

  12. Production et purification rapide d'une Taq polymérase pour les applications routinières de biologie moléculaire

    OpenAIRE

    Caromel, Bernard

    2016-01-01

    Cet article présente un protocole simple, rapide et économique de production et de purification d'une enzyme thermostable de type ADN polymérase (Taq polymérase) à destination des manipulations routinières de biologie moléculaire comme la PCR et ses diverses applications.

  13. Crystal structure of a 2:1 piroxicam-gentisic acid co-crystal featuring neutral and zwitterionic piroxicam mol-ecules.

    Science.gov (United States)

    Horstman, Elizabeth M; Bertke, Jeffery A; Woods, Toby J; Kenis, Paul J A

    2016-12-01

    A new 2:1 co-crystal of piroxicam and gentisic acid [systematic name: 4-hy-droxy-1,1-dioxo- N -(pyridin-2-yl)-2 H -1λ 6 ,2-benzo-thia-zine-3-carboxamide-2-(4-oxido-1,1-dioxo-2 H -1λ 6 ,2-benzo-thia-zine-3-amido)-pyridin-1-ium-2,5-di-hydroxy-benzoic acid, 2C 15 H 13 N 3 O 4 S·C 7 H 6 O 4 ] has been synthesized using a microfluidic platform and initially identified using Raman spectroscopy. In the co-crystal, one piroxicam mol-ecule is in its neutral form and an intra-molecular O-H⋯O hydrogen bond is observed. The other piroxicam mol-ecule is zwitterionic (proton transfer from the OH group to the pyridine N atom) and two intra-molecular N-H⋯O hydrogen bonds occur. The gentisic acid mol-ecule shows whole-mol-ecule disorder over two sets of sites in a 0.809 (2):0.191 (2) ratio. In the crystal, extensive hydrogen bonding between the components forms layers propagating in the ab plane.

  14. Precipitation and solidification of calzirtite in liquid phase sintered ZrO[sub 2] (10. 8 mol. %CaO) with TiO[sub 2] additions

    Energy Technology Data Exchange (ETDEWEB)

    Shen Pouyan; Hon Yungshon (Inst. of Materials Science and Engineering, National Sun Yat-Sen Univ., Kaohsiung (Taiwan))

    1992-12-30

    Microstructure of calzirtite (Ca[sub 2]Zr[sub 5]Ti[sub 2]O[sub 16]) in CaO-ZrO[sub 2]-TiO[sub 2] ceramics was studied by transmission electron microscopy. The ceramics were prepared by liquid phase sintering (1600degC, 6h) powders of calcia-partially stabilized zirconia (PSZ of composition ZrO[sub 2]-10.8mol.%CaO and phase assemblages of tetragonal (t) ZrO[sub 2] in the cubic (c) ZrO[sub 2] matrix) and adding to them 2 mol.%, 4 mol.% and 8 mol.% TiO[sub 2] (designated specimens 2T, 4T and 8T respectively). Calzirtite was not formed in specimen 2T, but formed in the grain-boundary liquid of specimens 4T and 8T by epitaxial nucleation from ZrO[sub 2] followed cellular of spherulitic growth to form plate-like variants with [l brace]200[r brace] habit plane which elongated predominantly along the crystallographic c axis. For specimens 8T, calzirtite was formed in the shell region of the PSZ grains and appeared more or less equiaxed by domain nucleation within the c-ZrO[sub 2] matrix. In addition to the effect of interfacial energy, structural similarity was crucial for nucleating calzirtite epitaxially from ZrO[sub 2]. (orig.).

  15. RD-MolPack technology for the constitutive production of self-inactivating lentiviral vectors pseudotyped with the nontoxic RD114-TR envelope.

    Science.gov (United States)

    Marin, Virna; Stornaiuolo, Anna; Piovan, Claudia; Corna, Stefano; Bossi, Sergio; Pema, Monika; Giuliani, Erica; Scavullo, Cinzia; Zucchelli, Eleonora; Bordignon, Claudio; Rizzardi, Gian Paolo; Bovolenta, Chiara

    2016-01-01

    To date, gene therapy with transiently derived lentivectors has been very successful to cure rare infant genetic diseases. However, transient manufacturing is unfeasible to treat adult malignancies because large vector lots are required. By contrast, stable manufacturing is the best option for high-incidence diseases since it reduces the production cost, which is the major current limitation to scale up the transient methods. We have previously developed the proprietary RD2-MolPack technology for the stable production of second-generation lentivectors, based on the RD114-TR envelope. Of note, opposite to vesicular stomatitis virus glycoprotein (VSV-G) envelope, RD114-TR does not need inducible expression thanks to lack of toxicity. Here, we present the construction of RD2- and RD3-MolPack cells for the production of self-inactivating lentivectors expressing green fluorescent protein (GFP) as a proof-of-concept of the feasibility and safety of this technology before its later therapeutic exploitation. We report that human T lymphocytes transduced with self-inactivating lentivectors derived from RD3-MolPack cells or with self-inactivating VSV-G pseudotyped lentivectors derived from transient transfection show identical T-cell memory differentiation phenotype and comparable transduction efficiency in all T-cell subsets. RD-MolPack technology represents, therefore, a straightforward tool to simplify and standardize lentivector manufacturing to engineer T-cells for frontline immunotherapy applications.

  16. Biochemical changes during aging of soybean seed

    Directory of Open Access Journals (Sweden)

    Balešević-Tubić Svetlana

    2009-01-01

    Full Text Available Biochemical changes that occur in the seed as a result of ageing are very significant for seed quality and longevity. Because of its characteristic composition, processes occurring in the seed of oil crops during storage will be typical as well. Six soybean varieties developed in Institute of field and vegetable crops Novi Sad, submitted to accelerated and natural aging, under controlled and conventional storage conditions were used in these trials. The content of malondialdehyde, superoxide dismutase and peroxidase activities were studied. The biochemical processes i.e. lipid peroxidation, as well as the decrease in supeoxide dismutase and peroxidase activities (especially pronounced by applied accelerated aging were caused by both type of aging. The degree of seed damage and the ability of seed to resist the negative consequences of aging were influenced, beside duration of aging period, by type of storage and characteristics of soybean varieties. .

  17. Estándares de fermentación y maduración artesanal de Bioles

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    Tulio Orellana

    2016-06-01

    Full Text Available Esta investigación, tuvo como objetivo principal establecer indicadores de calidad en el proceso  de  fermentación  anaerobia  de  bioles  de producción  local  y  conocer  sus principales  componentes   químicos. Se  realizó  la  determinación  periódica   de  los parámetros  físico químicos  "in  situ"  y  en  laboratorio,  luego  se realizó  un  tamizaje químico,  evaluándose  además  su  actividad fertilizante  mediante  la determinación  de macro  y  micronutrientes.  Se  observó  que  los bioles  con  bioactivad  presentan  los siguientes intervalos de confianza en sus parámetros físico-químicos: pH entre 3,4 y 4,6; conductividad  entre  16,2 y  23,84  mS/cm;  densidad  entre  1,030 y 1,060 Kglm3. El tamizaje químico permitió determinar de forma preliminar la presencia de los siguientes grupos químicos: alcaloides, terpenos/esteroides, quinonas, fenoles y taninos, entre otros, algunos de los cuales han sido asociados a su actividad antimicrobiana y promotora del crecimiento vegetal, constituyendo estos resultados los primeros generados en el país sobre el estudio de bioles de producción local, permitiendo el diseño de una metodología útil para la evaluación de otros bioinsumos de uso agrícola. Para el análisis estadístico del proceso de producción, se obtuvo un modelo de regresión polinómica de fado dos, para cada variable;  además del coeficiente de determinación del modelo (R-. También  se analizó la tendencia central y dispersión de las variables (parámetro de calidad, a través del promedio y la desviación estándar en el proceso y producto terminado.

  18. Biological control of mycotoxin-producing molds Controle biológico de fungos de armazenamento produtores de micotoxinas

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    Flávio Henrique Vasconcelos de Medeiros

    2012-10-01

    , mas a maioria deles se baseia no uso de fungicidas. Embora os produtos químicos sejam, muitas vezes, a primeira estratégia de defesa contra fungos micotoxigênicos, o uso indiscriminado de fungicidas vem despertando a percepção pública, em razão de seus efeitos nocivos sobre o meio ambiente e à saúde humana/animal. Assim, existe uma crescente pressão pública em busca de alternativas mais segura e não nocivas ao meio ambiente para controlar estes organismos. Nesse contexto, o controle biológico utilizando antagonistas microbianos, tais como bactérias, fungos e leveduras têm mostrado ser um substituto viável para reduzir a utilização de produtos químicos. Apesar dos resultados positivos, usando os agentes de controle biológico, poucos produtos apenas foram registrados e estão comercialmente disponíveis para controlar fungos produtores de micotoxinas. Esta revisão traz estratégias de controle biológico para evitar ou reduzir danos em commodities agrícolas causadas por fungos de armazenamento e a contaminação de alimentos e rações por micotoxinas.

  19. Exposición biológica a patógenos hemáticos y temporalidad laboral

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    Brígido Pérez Bermúdez

    2007-06-01

    Full Text Available La exposición biológica a patógenos hemáticos (E.B.P.H. y especialmente el pinchazo es el accidente más frecuente en el ámbito sanitario. Objetivos: El objetivo del estudio es comprobar la mayor siniestralidad en este tipo de accidentes entre los trabajadores eventuales y analizar si existe un perfil determinado. Metodología: Se analizaron todos los accidentes con riesgo biológico ocurridos durante un año en el Hospital Universitario de la Princesa de Madrid. Se construyó un modelo de regresión logística cuya variable dependiente es el tipo de contrato (eventual o plaza en propiedad, las variables independientes estudiadas fueron: edad, sexo, categoría, lugar del accidente, antigüedad en el puesto, anatomía lesionada, agente que lo produce, turno de trabajo, forma en el que se produjo, mes y día de accidente. Resultado: Existe un riesgo relativo de 1.96 (1.48-2.60 de producirse un E.B.P.H.en un "eventual". En el análisis multivariante no se observan diferencias significativas entre ambos tipos de trabajadores, excepto el aumento de E.B.P.H. ocurridos entre los trabajadores de plantilla en el mes de Septiembre. Conclusiones: Aunque existe casi dos veces más riesgo de que exista un accidente biológico entre los eventuales, no existen diferencias significativas en las variables estudiadas en los dos tipos de trabajadores.Blood pathogens biological exposure (B.P.B.O and specially needle stick injury is the most common accident in the sanitary field. Objectives: The main objective of this research is to check the level of sinestry in this kind of accidents among temporary workers and analyse if there is a certain profile. Methodology: All accidents with biologic risks happened in a year were analised in the University Hospital "La Princesa" . A logistic regression was performed where the dependent variables to study were: age, sex, grade, place of the accident, seniority, injured body part, producing agent, working shift, way in

  20. Fitness effects of fluctuations in biochemical networks

    Science.gov (United States)

    Tanase-Nicola, Sorin

    2009-03-01

    The concentration of many cellular components fluctuates not only as a response to external and internal inputs but also due to random birth and death events of individual molecules. This biochemical noise affects the capacity of every individual cell in a population to respond and adapt to the environment. While the sources and effects of biochemical fluctuations on individual cells have been intensively studied, the effects of noise on the growth rate of a population of cells are much less understood. We present a model of the cell cycle in which the growth and division of individual cells are coupled with the noisy dynamics of their internal components. The model allows us to compute the contribution of the biochemical noise to the average growth rate of a population of cells as a function of the noise strength and the correlation time of the fluctuations. We show that, due to fluctuations, the growth rate of a population of cells is always larger than the average growth rate of a individual cell and can be larger even than a corresponding deterministic model. In most relevant cases it is assumed that the average concentration of a cellular component is close to a value that maximizes the population growth as given by the external, environmental, conditions and the internal cellular regulation. In such cases we show that contribution of fluctuations to the growth rate is negative and increases with the sensitivity of the biochemical networks to the noise sources and the noise correlation time. We also discuss how the selection pressure due to fluctuations affects the structure and parameters of genetic regulatory networks.

  1. The relationship between advanced oxidation protein products (AOPP) and biochemical and histopathological findings in patients with nonalcoholic steatohepatitis.

    Science.gov (United States)

    Ozenirler, Seren; Erkan, Gulbanu; Konca Degertekin, Ceyla; Ercin, Ugur; Cengiz, Mustafa; Bilgihan, Ayse; Yilmaz, Guldal; Akyol, Gulen

    2014-03-01

    To investigate the correlation between advanced oxidation protein products (AOPP) levels and biochemical and histopathological findings in patients with nonalcoholic steatohepatitis (NASH). Sixty biopsy-proven NASH patients and 60 individuals with ultrasonographically healthy liver (the control group) were included in the study. AOPP levels were determined in all the participants and liver histopathological examination based on liver biopsy was performed in NASH patients. The NASH activity score (NAS), hepatosteatosis, liver inflammation and fibrosis were evaluated. Serum AOPP level was significantly higher in the NASH group than that in the control group (461.8 ± 201.9 μmol/L vs 191.7 ± 152.5 μmol/L, P < 0.001). The receiver operating characteristic (ROC) curve revealed a sensitivity of 73.3% and a specificity of 88.3% for the diagnosis of NASH with an AOPP cut-off value of 332 μmol/L (the area under ROC curve 0.88, 95% confidence interval 0.82-0.94, P < 0.01). AOPP levels were positively correlated with NAS (r = 0.27, P = 0.035), fibrosis (r = 0.27, P = 0.037) and inflammation (r = 0.34, P = 0.008), but not the grade of steatosis (r = 0.02, P = 0.83) or ballooning (r = 0.02, P = 0.55). AOPP levels are significantly higher in patients with NASH than in those with ultrasonographically healthy liver. AOPP levels are positively correlated with biochemical and histopathological findings (NAS, liver inflammation and fibrosis), indicating that AOPP may play a role in the development of liver fibrosis and inflammation and may predict liver histopathology in NASH. © 2013 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.

  2. Novel mutations in xanthine dehydrogenase/oxidase cause severe hypouricemia: biochemical and molecular genetic analysis in two Czech families with xanthinuria type I.

    Science.gov (United States)

    Stiburkova, Blanka; Krijt, Jakub; Vyletal, Petr; Bartl, Josef; Gerhatova, Eva; Korinek, Martin; Sebesta, Ivan

    2012-01-18

    The article describes the clinical, biochemical, enzymological and molecular genetics findings in two patients from two families with xanthinuria type I. Biochemical analysis using high performance liquid chromatography, allopurinol loading test and analysis of xanthine oxidase activity in plasma and of uromodulin excretion in urine were performed. Sequencing analysis of the xanthine dehydrogenase gene and the haplotype and statistical analyses of consanguinity were performed. Probands showed extremely low concentrations of uric acid, on seven occasions under the limit of detection. The concentration of uric acid in 38-year-old female was 15 μmol/L in serum and 0.04 mmol/L in urine. Excretion of xanthine in urine was 170 mmol/mol creatinine. The concentration of uric acid in 25-year-old male was 0.03 mmol/L in urine. Excretion of xanthine in urine was 141 mmol/mol creatinine. The allopurinol loading test confirmed xanthinuria type I. The xanthine oxidase activities in patients were 0 and 0.4 pmol/h/mL of plasma. We found three nonsense changes: p.P214QfsX4 and unpublished p.R825X and p.R881X. We found two nonconsanguineous compound heterozygotes with xanthinuria type I caused by three nonsense changes. The methods used did not confirm consanguinity in the probands, thus there might be an unconfirmed biological relationship or mutational hotspot. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca.

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    Matthias Engleder

    Full Text Available Kievitone hydratase catalyzes the addition of water to the double bond of the prenyl moiety of plant isoflavonoid kievitone and, thereby, forms the tertiary alcohol hydroxy-kievitone. In nature, this conversion is associated with a defense mechanism of fungal pathogens against phytoalexins generated by host plants after infection. As of today, a gene sequence coding for kievitone hydratase activity has only been identified and characterized in Fusarium solani f. sp. phaseoli. Here, we report on the identification of a putative kievitone hydratase sequence in Nectria haematococca (NhKHS, the teleomorph state of F. solani, based on in silico sequence analyses. After heterologous expression of the enzyme in the methylotrophic yeast Pichia pastoris, we have confirmed its kievitone hydration activity and have assessed its biochemical properties and substrate specificity. Purified recombinant NhKHS is obviously a homodimeric glycoprotein. Due to its good activity for the readily available chalcone derivative xanthohumol (XN, this compound was selected as a model substrate for biochemical studies. The optimal pH and temperature for hydratase activity were 6.0 and 35°C, respectively, and apparent Vmax and Km values for hydration of XN were 7.16 μmol min-1 mg-1 and 0.98 ± 0.13 mM, respectively. Due to its catalytic properties and apparent substrate promiscuity, NhKHS is a promising enzyme for the biocatalytic production of tertiary alcohols.

  4. Biochemical changes in cuttings of Robinia pseudoacacia after treatment with naphthenate

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    SLAVKO KEVRESAN

    2007-10-01

    Full Text Available Naphthenic acids were isolated from gas oil fractions (distillation interval 168–290 °C of Vojvodina crude oil “Velebit”, characterized and their biological ac­tivity evaluated by the biochemical changes in cuttings of Robinia pseudoacacia after treatment with naphthenate. The activities of IAA peroxidase, total peroxi­da­ses and amylase, as well as the contents of reducing sugars and total proteins, were determined in the basal parts of soft wood cuttings of black locust after treat­ment with sodium naphthenate or the sodium salt of 1-naphthaleneacetic acid (NAA, con­­centration 10-7 mol dm-3 for 3 or 6 h. High activities of IAA oxidase and amy­lase, together with a low activity of peroxidase (which is known as being stimula­tory for the initiation and activation of primordia were obtained after the three-hour treatment with sodium naphthenate. Six-hour treatment had an inhibitory effect on the examined biochemical markers. The effects of three- and six-hour treatments with NAA were between those of the corresponding treatment with naphthenic acids.

  5. Physiological and biochemical responses of Synechococcus sp. PCC7942 to Irgarol 1051 and diuron.

    Science.gov (United States)

    Deng, Xiangyuan; Gao, Kun; Sun, Junlong

    2012-10-15

    Cyanobacteria are prokaryotic algae found in oceans and freshwaters worldwide. These organisms are important primary producers in aquatic ecosystems because they can provide essential food for grazers and herbivores. In this study, the physiological and biochemical responses of the freshwater cyanobacterium Synechococcus sp. PCC7942 to two organic booster biocides Irgarol 1051 and diuron were compared and evaluated using 96 h growth tests in a batch-culture system. The 96 h median effective concentrations (EC(50)) were 0.019 and 0.097 μmol L(-1) for Irgarol 1051 and diuron, respectively, which indicate that Irgarol 1051 is about 5 times more toxic than diuron to cyanobacteria. Moreover, remarkable physiological and biochemical responses occurred in the Irgarol 1051 and diuron treatments. Irgarol 1051 and diuron stimulated cyanobacterial growth, increased the soluble protein content, and enhanced the catalase (CAT) activity at low concentrations, but inhibited them at high concentrations. However, the malondialdehyde (MDA) and polysaccharide content of the cyanobacteria were only significantly affected by Irgarol 1051. These observations suggest that Irgarol 1051 and diuron are toxic to Synechococcus sp. PCC7942, and their use should be restricted in maritime industries. Copyright © 2012 Elsevier B.V. All rights reserved.

  6. Multidimensional biochemical information processing of dynamical patterns.

    Science.gov (United States)

    Hasegawa, Yoshihiko

    2018-02-01

    Cells receive signaling molecules by receptors and relay information via sensory networks so that they can respond properly depending on the type of signal. Recent studies have shown that cells can extract multidimensional information from dynamical concentration patterns of signaling molecules. We herein study how biochemical systems can process multidimensional information embedded in dynamical patterns. We model the decoding networks by linear response functions, and optimize the functions with the calculus of variations to maximize the mutual information between patterns and output. We find that, when the noise intensity is lower, decoders with different linear response functions, i.e., distinct decoders, can extract much information. However, when the noise intensity is higher, distinct decoders do not provide the maximum amount of information. This indicates that, when transmitting information by dynamical patterns, embedding information in multiple patterns is not optimal when the noise intensity is very large. Furthermore, we explore the biochemical implementations of these decoders using control theory and demonstrate that these decoders can be implemented biochemically through the modification of cascade-type networks, which are prevalent in actual signaling pathways.

  7. Explorations into Chemical Reactions and Biochemical Pathways.

    Science.gov (United States)

    Gasteiger, Johann

    2016-12-01

    A brief overview of the work in the research group of the present author on extracting knowledge from chemical reaction data is presented. Methods have been developed to calculate physicochemical effects at the reaction site. It is shown that these physicochemical effects can quite favourably be used to derive equations for the calculation of data on gas phase reactions and on reactions in solution such as aqueous acidity of alcohols or carboxylic acids or the hydrolysis of amides. Furthermore, it is shown that these physicochemical effects are quite effective for assigning reactions into reaction classes that correspond to chemical knowledge. Biochemical reactions constitute a particularly interesting and challenging task for increasing our understanding of living species. The BioPath.Database is a rich source of information on biochemical reactions and has been used for a variety of applications of chemical, biological, or medicinal interests. Thus, it was shown that biochemical reactions can be assigned by the physicochemical effects into classes that correspond to the classification of enzymes by the EC numbers. Furthermore, 3D models of reaction intermediates can be used for searching for novel enzyme inhibitors. It was shown in a combined application of chemoinformatics and bioinformatics that essential pathways of diseases can be uncovered. Furthermore, a study showed that bacterial flavor-forming pathways can be discovered. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Multidimensional biochemical information processing of dynamical patterns

    Science.gov (United States)

    Hasegawa, Yoshihiko

    2018-02-01

    Cells receive signaling molecules by receptors and relay information via sensory networks so that they can respond properly depending on the type of signal. Recent studies have shown that cells can extract multidimensional information from dynamical concentration patterns of signaling molecules. We herein study how biochemical systems can process multidimensional information embedded in dynamical patterns. We model the decoding networks by linear response functions, and optimize the functions with the calculus of variations to maximize the mutual information between patterns and output. We find that, when the noise intensity is lower, decoders with different linear response functions, i.e., distinct decoders, can extract much information. However, when the noise intensity is higher, distinct decoders do not provide the maximum amount of information. This indicates that, when transmitting information by dynamical patterns, embedding information in multiple patterns is not optimal when the noise intensity is very large. Furthermore, we explore the biochemical implementations of these decoders using control theory and demonstrate that these decoders can be implemented biochemically through the modification of cascade-type networks, which are prevalent in actual signaling pathways.

  9. Ratos isogênicos F344 como modelo biológico de sepsis intra-abdominal

    Directory of Open Access Journals (Sweden)

    Sueli Blanes Damy

    2002-01-01

    Full Text Available Com o objetivo de estudar um modelo biológico de sepsis intra-abdominal aguda para estudos experimentais, foram infectados ratos isogênicos F344, convencionais, com a bactéria Escherichia coli (E.coli, cepa ATCC 11775, sorotipo H7:O1:K1. Os animais inoculados, machos e fêmeas, apresentaram 6 horas após a inoculação por E.coli os seguintes sintomas: arqueamento do dorso, piloereção, hiperpnéia e diminuição das atividades motoras. A dose que produziu 50% de mortalidade (DL50 após 7 dias, determinada pelo método Reed & Muench, foi de 6 x 10(5 CFU/ml (analisado em 32 machos e 32 fêmeas. A maior concentração de mortalidade foi observada nas primeiras 24 horas. A disfunção hepática, comum em sepsis intra-abdominal, foi avaliada por provas enzimáticas, em 0, 24, 48 e 168 horas após a inoculação. O estudo da migração de células polimorfonucleares-neutrófilos (PMN e mononucleares-macrófagos (MN apontou um aumento significante de PMN entre o grupos de machos (z ³ 4,7; p < 0,003 e de fêmeas (z ³ 6,2; p < 0,0003 inoculados E.coli, quando comparados ao grupos controles. Quanto às células MN, não houve diferença entre os grupos inoculados e os controles, tanto para os machos (z=2,3; p = 0,0107, como para as fêmeas (z=1,8; p =0,0359. Em conclusão, estes resultados demonstram que os ratos isogênicos F344 são modelos biológicos adequados para estudos de sepsis intra-abdominal aguda.

  10. Redução química e biológica do cromo hexavalente aplicado ao solo

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    D. D. Castilhos

    2001-06-01

    Full Text Available Realizou-se um experimento em laboratório, com o objetivo de avaliar não só a redução do cromo hexavalente aplicado ao solo com ênfase ao efeito da aplicação de carbono orgânico, manganês divalente, mas também a participação da microbiota do solo. Utilizaram-se, como unidades experimentais, amostras de 50 g de um Argissolo, acondicionadas em sacos de polipropileno e incubadas durante 42 dias com calcário para elevar o pH a 6,0 (2,0 t ha-1 de CaCO3 + MgCO3 na proporção de 2:1, Cr6+, esterco bovino e Mn2+ nas doses de 20, 50 e 40 mg kg-1, respectivamente. Metade das amostras foi sujeita à esterilização por autoclavagem com o objetivo de eliminar a atividade biológica. A aplicação de esterco bovino e sulfato de manganês promoveu a total redução do cromo hexavalente para cromo trivalente (Cr6+ → Cr3+ no solo, em 42 dias. Os modelos, quadrático e exponencial, foram os que melhor descreveram a cinética de redução com o tempo. A redução do Cr6+ foi estimulada pela atividade microbiana, sendo 16% maior em amostras de solo não esterilizadas, que continham esterco bovino, em comparação com as mesmas amostras esterilizadas por autoclavagem. Os resultados demonstraram que a descontaminação do solo que contém teores tóxicos de Cr6+ pode ser viabilizada tanto pela incorporação de adubo orgânico como pela manutenção de sua atividade biológica.

  11. La Evolución biológica en la Biofilosofía de F. J. Ayala

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    Diego Cano Espinosa

    2016-02-01

    Full Text Available El discurso biológico de Ayala se centra en estas tres cuestiones: 1 El hecho de la evolución. 2 Historia de la evolución. 3 Mecanismos del desarrollo y cambio evolutivos. El hecho determinante y cierto, según Ayala, es la evidencia de relaciones entre todos los organismos debidas a una común descendencia con modificaciones como lo han demostrado con abundancia la Paleontología, Anatomía comparada, Biogeografía, Embriología, Genética Molecular, Bioinformática y otras disciplinas biológicas que nos llevan a la afirmación de que el hecho de la evolución de los seres vivos tiene el mismo grado de certeza que poseen los hechos históricos y científicos ampliamente confirmados. Para Ayala, explicaciones acerca del origen del mundo, del hombre y de otras criaturas se encuentran en todas las culturas. Tras la publicación del Origen de las Especies de C. Darwin en 1859, se produce gran polémica en los medios científicos, culturales y religiosos, pero pasado un período de maduración, el darwinismo logra ser aceptado especialmente bajo las formas de Neo-darwinismo y Teoría Sintética. Otro asunto muy distinto son las diversas teorías y micro-teorías que se proponen para explicar los mecanismos de la evolución de los organismos vivos que presentan todavía profundas lagunas, deficiencias y controversias que han provocado la aparición de teorías alternativas. No obstante, el darwinismo moderno constituye un peldaño importante en la reconstrucción del sinuoso y complejo proceso evolutivo de la vida a través de millones de años acudiendo a una razonable explicación causal sin que esto atente contra ningún supuesto religioso.

  12. 40 CFR 158.2000 - Biochemical pesticides definition and applicability.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Biochemical pesticides definition and applicability. 158.2000 Section 158.2000 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Biochemical Pesticides § 158.2000 Biochemical pesticides...

  13. Dactylifera L) on the biochemical indicators of lead poisoning in ...

    African Journals Online (AJOL)

    ... in the biochemical parameters. Thus, the treatment by the pectin of dates reduced the high concentration of these parameters. Our results show that the pectins of dates may have a corrective effect on the biochemical disturbances induced by the lead. Keywords: Phoenix Dactylifera, Pectin, Lead, Biochemical parameters ...

  14. Acidentes de trabalho com material biológico ocorridos em municípios de Minas Gerais

    Directory of Open Access Journals (Sweden)

    Renata Siqueira Julio

    2014-01-01

    Full Text Available O objetivo do estudo foi identificar o perfil dos acidentes com exposição a material biológico ocorridos em Minas Gerais. Estudo descritivo, transversal realizado por meio de consulta ao Sistema de Informação de Agravos de Notificação de 50 municípios do sul do Estado de Minas Gerais, Brasil, no período de 2007-2011. Foram registrados 460 acidentes, sendo que cerca da metade ocorreram entre auxiliares e técnicos de enfermagem, seguidos por enfermeiros e médicos. Houve predomínio dos acidentes ocorridos devido ao descarte inadequado de material perfurocortante. Entre os pacientes-fonte, verificou-se uma prevalência de 8,0% de sorologia reagente para o HIV; 1,0% para HBsAg; 6,0% para anti-HBC e 3% para o anti-HCV. Entre os acidentados, 14,0% não estavam imunizados para hepatite B, contudo, a prescrição de vacina e imunoglobulina foi inferior à necessidade. Os resultados subsidiarão o planejamento de ações preventivas e de novas condutas diante da ocorrência desse tipo de acidentes.

  15. Distancias biológicas entre una serie argentina y poblaciones mundiales según la dentición

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    Bollini, Gabriel A.

    2005-01-01

    Full Text Available Se comparan rasgos dentales discontinuos de una muestra de 469 cráneos procedentes de distintos puntos geográficos del territorio Argentino, con cronologías que no sobrepasan los 1500 años y se extienden hasta tiempos de la conquista y posteriores, con datos aportados por otros autores para distintas poblaciones mundiales (mongoloides sinodontos y sondadontos, caucasoides y negroides. Los rasgos analizados son: keilomorfía en incisivos superiores; estructura del hipocono y tubérculo de Carabelli en molares superiores; cúspides sexta-séptima y protostílido en molares inferiores. Se estima mediante la Medida Media de la Divergencia (MMD la distancia biológica entre la serie argentina y las restantes poblaciones mundiales. Los resultados obtenidos indican en la muestra estudiada la presencia de un fuerte componente original propio de las poblaciones mongoloides, como así también un alejamiento de la misma respecto a poblaciones negroides y caucasoides. Asimismo, la comparación con poblaciones mongoloides sondadontas y sinodontas de Asia y Australia muestra una mayor semejanza con los grupos sinodontos, corroborando la opinión de otros autores en el sentido de que los grupos americanos tienen mayor similitud en la estructura dentaria con las poblaciones propias del noreste asiático, lugar de origen del complejo dental mongoloide-sinodonte.

  16. Valoración de las aguas residuales mediante procedimientos analíticos y biológicos

    Directory of Open Access Journals (Sweden)

    M. Carballo

    2002-06-01

    Full Text Available Ciertos procedimientos, basados en aproximaciones analíticas y biológicas, están demostrando ser útiles en la valoración del riesgo de las aguas residuales urbanas procedentes de las Plantas de Tratamiento. Estos efluentes, considerados “mezclas complejas”, compuestos por sustancias de muy diferente naturaleza, origen y características toxicológicas y medio ambientales, requieren una valoración realista. Con el fin de colaborar al conocimiento de una parte de la realidad de nuestro país, presentamos un estudio sobre once depuradoras urbanas en las que se ha realizado un perfil de compuestos orgánicos y una valoración toxicológica mediante tests de toxicidad agudos, crónicos, de estrogenicidad, mutagenicidad y teratogenia. Los resultados muestran que 7 efluentes presentan toxicidad aguda, 3 toxicidad crónica y 4 estrogenicidad. Destacamos el hecho de que los 4 efluentes que presentan estrogenicidad, poseen al menos 3 de las sustancias estrogénicas detectadas mediante el perfil cromatográfico. Este tipo de consideraciones nos hace reflexionar sobre la necesidad de incorporar este tipo de metodologías para disponer de un conocimiento más realista de estas situaciones.

  17. Reajustando o limite de tolerância biológica aplicado à plumbemia no Brasil

    Directory of Open Access Journals (Sweden)

    Ricardo Cordeiro

    Full Text Available Foram alocados aleatoriamente vinte trabalhadores expostos ocupacionalmente ao chumbo em uma indústria de acumuladores elétricos de médio porte, no interior do Estado de São Paulo, os quais apresentavam plumbemia e excreção urinária do ácido delta-aminolevulínico, nos últimos dois anos, sempre menores que 60 µg/dL e 10 mg/L, respectivamente. Os trabalhadores foram submetidos a eletroneurografia do nervo radial direito e a dosagem de plumbemia. Com estas medidas ajustou-se um modelo de regressão linear simples de primeira ordem, tendo como variável dependente a velocidade de condução e como variável independente a plumbemia. Analisando-se a regressão ajustada, infere-se que o valor preditivo negativo do limite de tolerância biológica brasileiro aplicado à plumbemia seja de apenas 0,63. O estudo sugere que o valor do referido limite de tolerância deva ser reduzido do atual valor de 60 µg/dL para 32 µg/dL, para ter um valor preditivo negativo de 0,99.

  18. Aspectos biológicos de Piezodorus guildinii (Westwood (Hemiptera: Pentatomidae criados con diferentes dietas en condiciones de laboratorio

    Directory of Open Access Journals (Sweden)

    Victor A. GOMEZ

    2013-01-01

    Full Text Available Se realizaron estudios de los aspectos biológicos de Piezodorus guildinii en condiciones de laboratorio; evaluándose el desarrollo de ninfas en cuatro dietas naturales y una artificial. En la dieta N1 (Phaseolus vulgaris se obtuvo una duración de 35,09 días y en la dieta N4 (Glycine max, Arachis hipogaea y Phaseolus vulgaris fue menor, con 23,60 días; observándose también una menor mortalidad (20% con la misma dieta. Las dietas artificial y natural con maní (A. hipogaea no permitieron el desarrollo de ninfas. En la fase adulta se evaluaron tres dietas naturales; el ciclo total fue mayor en la dieta N1 con 40,20 días y en la dieta N4 con 28,80 días. El periodo de preoviposición fue menor en la dieta N6 (G. max, A. hipogaea, P. vulgaris y Ligustrum lucidum con 11,13 días; en la dieta N4 y N6 el promedio de masas de huevo fue similar con 9,2 y 9,4 respectivamente observándose una viabilidad superior en la dieta N6 con un promedio del 80%.

  19. Cachaza y carbonilla: residuos agroindustriales con potencial de fertilización biológica nitrogenada

    Directory of Open Access Journals (Sweden)

    Sandra Patricia Montenegro Gómez

    2015-03-01

    Full Text Available Fue evaluada la presencia de bacterias fijadoras de nitrógeno (diazotróficas en tres mezclas de residuos con proporciones diferentes de compost de cachaza, arena y carbonilla; proporciones iguales de cascarilla de arroz y bagazo de caña:M1 (40, 10, 10, 20, 20 respectivamente, M2 (20, 0, 40, 20, 20 respectivamente, M3 (10, 40, 10, 20, 20 respectivamente. La evaluación se basó en la presencia del gen nifH, característico exclusivamente en bacterias diazotróficas. Se utilizó como control positivo muestras de ADN de bacterias del genero Azotobacter spp. Las mezclas con mayor contenido de cachaza y carbonilla M1 y M2, amplificaron para el gen nifH. Las bandas más fuertes se observan en M1 con mayor contenido de compost de cachaza. En contraste la mezcla M3, conformada principalmente por arena y cantidad muy inferior de residuos orgánicos no amplificó; sugiriendo que los contenidos de materia orgánica,son relevantes para la presencia de bacterias diazotróficas y potencial estimulante de fijación biológica de nitrógeno.

  20. Caracterización de recubrimientos DLC/Si bajo la influencia de un fluido biológico simulado

    Directory of Open Access Journals (Sweden)

    William Vargas

    2016-01-01

    Full Text Available Uno de los materiales más usados como biomaterial es el acero 316LVM; sin embargo, presenta complicaciones al trabajarlo como reemplazo permanente, debido a que libera iones metálicos a los tejidos, generando aumento en el número de intervenciones que deben realizarse; ante esto, se estudia cómo mejorar el comportamiento de materiales convencionales mediante recubrimientos que elevan sus propiedades mecánicas y anticorrosivas, incrementando su vida útil. Se evaluó el comportamiento ante el fenómeno de micro-abrasión-corrosión de recubrimientos DLC/Si depositados mediante la técnica de deposición química de vapor asistida por plasma en contacto con solución de Ringer, simulando un ambiente biológico. Los resultados indican que el recubrimiento experimenta un aumento en el volumen de desgaste cuando se encuentra sometido al mecanismo de micro-abrasión-corrosión en relación con la prueba de solo microabrasión, además, la pérdida más significativa se encuentra en el sustrato sin recubrimiento; esto demuestra el efecto protector del recubrimiento.

  1. O corpo e seus limites: entre o tecnicismo biológico e a narrativa estética

    Directory of Open Access Journals (Sweden)

    Clara Virgínia de Queiroz Pinheiro

    2011-01-01

    Full Text Available Na modernidade, o corpo foi tomado como um lugar reservado à identidade e ao espaço de diferenciação com os objetos do mundo. Essa marcação subjetiva do corpo pressupõe a referência à ideia de finitude, ou seja, aos condicionamentos biológicos e sócio-culturais do indivíduo, de acordo com a perspectiva foucaultiana sobre o surgimento das ciências humanas. Atualmente, em função dos desenvolvimentos da biotecnologia, a manipulação do corpo, explorada em seu caráter objetivo, tornou-se cada vez mais eficiente e autônoma em relação a qualquer restrição individual. Em face desses discursos biotecnológicos, posiciona-se uma experimentação literária do corpo que o aborda em sua materialidade, mas a partir de uma narrativa que inscreve nas células e órgãos recortes de um sujeito. Dessa forma, se reconhece que, a propósito da dimensão subjetiva do corpo, as intervenções tecnocientíficas ainda não determinaram completamente uma descontinuidade entre a modernidade e a chamada "pós-modernidade".

  2. Parastacuspugnax (Poeppig, 1835 (Crustacea, Decapoda, Parastacidae: conocimiento biológico, presión extractiva y perspectivas de cultivo

    Directory of Open Access Journals (Sweden)

    Erich H Rudolph

    2013-09-01

    Full Text Available El camarón excavador, Parastacuspugnax (Poeppig, 1835, es una de las seis especies de la familia Parastacidae que habita las aguas dulces de Chile, y la que soporta mayor extracción para consumo humano. Sin embargo, carece de una legislación que la proteja y su conocimiento biológico es escaso, fragmentario y disperso. En este artículo se recopila la información publicada de esta especie, junto a otros datos accesibles al autor, con el objetivo de actualizar el conocimiento de su biología, evidenciar la magnitud de la presión extractiva que soporta y analizar la factibilidad de su cultivo en cautiverio. Se proporciona información sobre: sistemática, distribución geográfica, hábitat, conducta excavadora, variación morfológica, casos teratológicos, tamaño, relación longitud-peso, estructura de longitud, densidad poblacional, biología reproductiva, migraciones, hábitos alimentarios, crecimiento, depredadores, epibiontes, parásitos, enfermedades, pesquería y comercialización, legislación, estado de conservación y perspectivas de cultivo.

  3. On the Adaptive Design Rules of Biochemical Networks in Evolution

    Directory of Open Access Journals (Sweden)

    Bor-Sen Chen

    2007-01-01

    Full Text Available Biochemical networks are the backbones of physiological systems of organisms. Therefore, a biochemical network should be sufficiently robust (not sensitive to tolerate genetic mutations and environmental changes in the evolutionary process. In this study, based on the robustness and sensitivity criteria of biochemical networks, the adaptive design rules are developed for natural selection in the evolutionary process. This will provide insights into the robust adaptive mechanism of biochemical networks in the evolutionary process. We find that if a mutated biochemical network satisfies the robustness and sensitivity criteria of natural selection, there is a high probability for the biochemical network to prevail during natural selection in the evolutionary process. Since there are various mutated biochemical networks that can satisfy these criteria but have some differences in phenotype, the biochemical networks increase their diversities in the evolutionary process. The robustness of a biochemical network enables co-option so that new phenotypes can be generated in evolution. The proposed robust adaptive design rules of natural selection gain much insight into the evolutionary mechanism and provide a systematic robust biochemical circuit design method of biochemical networks for biotechnological and therapeutic purposes in the future.

  4. Biochemical Network Stochastic Simulator (BioNetS: software for stochastic modeling of biochemical networks

    Directory of Open Access Journals (Sweden)

    Elston Timothy C

    2004-03-01

    Full Text Available Abstract Background Intrinsic fluctuations due to the stochastic nature of biochemical reactions can have large effects on the response of biochemical networks. This is particularly true for pathways that involve transcriptional regulation, where generally there are two copies of each gene and the number of messenger RNA (mRNA molecules can be small. Therefore, there is a need for computational tools for developing and investigating stochastic models of biochemical networks. Results We have developed the software package Biochemical Network Stochastic Simulator (BioNetS for efficientlyand accurately simulating stochastic models of biochemical networks. BioNetS has a graphical user interface that allows models to be entered in a straightforward manner, and allows the user to specify the type of random variable (discrete or continuous for each chemical species in the network. The discrete variables are simulated using an efficient implementation of the Gillespie algorithm. For the continuous random variables, BioNetS constructs and numerically solvesthe appropriate chemical Langevin equations. The software package has been developed to scale efficiently with network size, thereby allowing large systems to be studied. BioNetS runs as a BioSpice agent and can be downloaded from http://www.biospice.org. BioNetS also can be run as a stand alone package. All the required files are accessible from http://x.amath.unc.edu/BioNetS. Conclusions We have developed BioNetS to be a reliable tool for studying the stochastic dynamics of large biochemical networks. Important features of BioNetS are its ability to handle hybrid models that consist of both continuous and discrete random variables and its ability to model cell growth and division. We have verified the accuracy and efficiency of the numerical methods by considering several test systems.

  5. Dispositivo de posicionamiento de muestras biológicas para su irradiación en un canal radial de un reactor nuclear // Biological samples positioning device for irradiations on a radial channel at the nuclear research reactor

    Directory of Open Access Journals (Sweden)

    Maritza Rodríguez - Gual

    2010-05-01

    Full Text Available ResumenPor la demanda de un dispositivo experimental para el posicionamiento de las muestras biológicaspara su irradiación en un canal radial de un reactor nuclear de investigaciones en funcionamiento, seconstruyó y se puso en marcha un dispositivo para la colocación y retirada de las muestras en laposición de irradiación de dicho canal. Se efectuaron las valoraciones económicas comparando conotro tipo de dispositivo con las mismas funciones. Este trabajo formó parte de un proyectointernacional entre Cuba y Brasil que abarcó el estudio de los daños inducidos por diferentes tipos deradiación ionizante en moléculas de ADN. La solución propuesta es comprobada experimentalmente,lo que demuestra la validez práctica del dispositivo. Como resultado del trabajo, el dispositivoexperimental para la irradiación de las muestras biológicas se encuentra instalado y funcionando yapor 5 años en el canal radial # 3(BH#3 Palabras claves: reactor nuclear de investigaciones, dispositivo para posicionamiento de muestras,___________________________________________________________________________AbstractFor the demand of an experimental device for biological samples positioning system for irradiationson a radial channel at the nuclear research reactor in operation was constructed and started up adevice for the place and remove of the biological samples from the irradiation channels withoutinterrupting the operation of the reactor. The economical valuations are effected comparing withanother type of device with the same functions. This work formed part of an international projectbetween Cuba and Brazil that undertook the study of the induced damages by various types ofionizing radiation in DNA molecules. Was experimentally tested the proposed solution, whichdemonstrates the practical validity of the device. As a result of the work, the experimental device forbiological samples irradiations are installed and operating in the radial beam hole #3(BH#3

  6. Dinámica de la molécula más importante de la vida: el ADN. I. Modelos lineales y no lineales

    Directory of Open Access Journals (Sweden)

    Lorena Ramírez Reyes

    2005-01-01

    Full Text Available En este artículo se revisan los modelos más simples para la molécula más importante dela vida: el ADN. Se estudian las excitaciones colectivas y paulatinamente van incorporándose en elanálisis cualidades complejas de la molécula. Esto conlleva al estudio de la dinámica de talesexcitaciones mediante ecuaciones diferenciales no lineales. Las soluciones especiales de estasecuaciones corresponden a un tipo de ondas no lineales que mantienen forma y velocidadconstantes con la mínima pérdida de energía e información, conocidas como solitones ocompactones.

  7. Col·lisions ió - molècula: reaccions químiques i agregats iònics

    OpenAIRE

    López Marne, Estefanía

    2015-01-01

    Aquesta Tesi tracta de les reaccions en fase gas generades per col•lisions entre ions i molècules neutres, estudiant per la vessant experimental i la teòric-computacional els processos plantejats. La memòria està dividida en dos grans blocs. El primer, dut a terme en el laboratori de Dinàmica de Reaccions Químiques de la UB, es centra en processos de deshidrohalogenació i deshidratació en molècules orgàniques (iso-C3H7Br, i iso-C3H7OH) induïts per els cations alcalins Li+, Na+ i I±; reaccions...

  8. Continuous and Discrete Stochastic Models of the F1-ATPase Molecular Motor / Modèles continu et discret du moteur moléculaire F1-ATPase

    OpenAIRE

    Gerritsma, Eric

    2010-01-01

    L'objectif de notre thèse de doctorat est d’étudier et de décrire les propriétés chimiques et mé- caniques du moteur moléculaire F1 -ATPase. Le moteur F1 -ATPase est un moteur rotatif, d’aspect sphérique et d’environ 10 nanomètre de rayon, qui utilise l’énergie de l’hydrolyse de l’ATP comme car- burant moléculaire. Des questions fondamentales se posent sur le fonctionnement de ce moteurs et sur la quantité de travail qu’il peut fournir. Il s’agit de questions qui conce...

  9. Biochemical Markers for Assessing Aquatic Contamination

    Directory of Open Access Journals (Sweden)

    Zdeňka Svobodová

    2007-11-01

    Full Text Available Biochemical markers, specifically enzymes of the first phase of xenobiotic transformation - cytochrome P450 and ethoxyresorufin-O-deethylase (EROD - were used to determine the quantities of persistent organic pollutants (POPs in fish muscle (PCB, HCB, HCH, OCS, DDT. Eight rivers were monitored (Orlice, Chrudimka, Cidlina, Jizera, Vltava, Ohře and Bílina; and the River Blanice was used as a control. The indicator species selected was the chub (Leuciscus cephalus L.. There were no significant differences in cytochrome P450 content between the locations monitored. The highest concentration of cytochrome P450 in fish liver was in the Vltava (0.241 nmol mg-1 protein, and the lowest was in the Orlice (0.120 nmol mg-1 protein. Analysis of EROD activity showed a significant difference between the Blanice and the Vltava (P< 0.05, and also between the Orlice and the Vltava (P< 0.01, the Orlice and the Bílina (P< 0.01, and the Orlice and the Ohře (P< 0.05. The highest EROD activity in fish liver was in the Vltava (576.4 pmol min-1 mg-1 protein, and the lowest was in the Orlice (63.05 pmol min-1 mg-1 protein. In individual locations, results of chemical monitoring and values of biochemical markers were compared. A significant correlation (P< 0.05 was found between biochemical markers and OCS, and PCB. Among the tributaries studied those that contaminated the Elbe most were the Vltava and the Bílina. These tributaries should not be considered the main sources of industrial contamination of the River Elbe, because the most important contamination sources were along the river Elbe itself.

  10. Diversidade de Agaricales (Basidiomycota na Reserva Biológica Walter Egler, Amazonas, Brasil Diversity of Agaricales (Basidiomycota in the Reserva Biológica Walter Egler, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Helenires Queiroz de Souza

    2004-01-01

    Full Text Available Foi realizado um estudo dos representantes da Ordem Agaricales Clements (Hymenomycetes, Basidiomycotina, ocorrentes na Reserva Biológica Walter Egler, situada na Estrada AM-010, Manaus-Itacoatiara, Km 64, Latitude 02° 43' S e Longitude 59° 47' W, Rio Preto da Eva, Amazonas. A área abrange 709 ha de floresta de terra firme primária. As coletas foram realizadas no período de dezembro de 2000 a junho de 2001 e seguiu-se a metodologia usual para identificação de Agaricales. Foram estudadas um total de 39 espécies, distribuídas em 13 gêneros e seis famílias: Polyporaceae: Pleurotus sp.; Hygrophoraceae: Hygrocybe cf. megistospora, Hygrocybe aff. miniceps, Hygrocybe occidentalis var. scarletina, e mais oito espécies de Hygrocybe indeterminadas; Tricholomataceae: Clitocybe sp., Hydropus sp.1 e Hydropus sp.2, Macrocystidia sp., Marasmiellus sp., Marasmius bellus, Marasmius haedinus var. haedinus,Marasmius cf. leoninus, Marasmius cf. mazatecus, Marasmius cf. ruber,Marasmius cf. setulosifolius, Marasmius tageticolor, Marasmius cf. variabiliceps var. variabiliceps, Marasmius sp.1, Marasmius sp.2, Marasmius sp.3 e Marasmius sp.4, Tricholoma sp.; Agaricaceae: Agaricus sp.1 e Agaricus sp.2, Lepiota sp., Cystoderma sp.; Entolomataceae: Entoloma cf. azureoviride, Entoloma cf. cystidiophorum, Entoloma strigosissima, Entoloma sp.; Russulaceae: Lactarius panuoides. Destas, Entoloma azureoviride, Hygrocybe miniceps, Lactarius panuoides, Marasmius cf. mazatecus, Marasmius cf. setulosifolius e Marasmius variabiliceps var. variabiliceps, provavelmente, estão sendo aqui citadas pela primeira vez, para o Brasil. Com exceção de Marasmius tageticolor, as demais espécies são citadas pela primeira vez, para a Reserva Egler. São fornecidas tabelas com a ocorrência das espécies de acordo com o gradiente topográfico (baixio, vertente, platô e seus respectivos habitats.A study of the order Agaricales Clements (Hymenomycetes, Basidiomycotina, occurring in

  11. [Chronic fatigue syndrome: biochemical examination of blood].

    Science.gov (United States)

    Hakariya, Yukiko; Kuratsune, Hirohiko

    2007-06-01

    Though patients with chronic fatigue syndrome (CFS) have lots of complaints, abnormal findings cannot be detected by biochemical screening tests. However, some specialized blood tests have revealed neuroendocrine immune axis abnormalities, which is closely associated with each other. Recent studies indicate that CFS can be understood as a special condition based on abnormality of the psycho-neuro-endocrino-immunological system, with the distinguishing feature of CFS seeming to be the secondary brain dysfunction caused by several cytokines and/or autoantibodies. In this paper, we summarize these abnormalities found in CFS and show the neuro-molecular mechanism leading to chronic fatigue.

  12. Conservation Laws in Biochemical Reaction Networks

    DEFF Research Database (Denmark)

    Mahdi, Adam; Ferragut, Antoni; Valls, Claudia

    2017-01-01

    We study the existence of linear and nonlinear conservation laws in biochemical reaction networks with mass-action kinetics. It is straightforward to compute the linear conservation laws as they are related to the left null-space of the stoichiometry matrix. The nonlinear conservation laws...... are difficult to identify and have rarely been considered in the context of mass-action reaction networks. Here, using the Darboux theory of integrability, we provide necessary structural (i.e., parameterindependent) conditions on a reaction network to guarantee the existence of nonlinear conservation laws...

  13. The biochemical womb of schizophrenia: A review.

    Science.gov (United States)

    Gaur, N; Gautam, S; Gaur, M; Sharma, P; Dadheech, G; Mishra, S

    2008-10-01

    The conclusive identification of specific etiological factors or pathogenic processes in the illness of schizophrenia has remained elusive despite great technological progress. The convergence of state-of-art scientific studies in molecular genetics, molecular neuropathophysiology, in vivo brain imaging and psychopharmacology, however, indicates that we may be coming much closer to understanding the genesis of schizophrenia. In near future, the diagnosis and assessment of schizophrenia using biochemical markers may become a "dream come true" for the medical community as well as for the general population. An understanding of the biochemistry/ visa vis pathophysiology of schizophrenia is essential to the discovery of preventive measures and therapeutic intervention.

  14. Simplifying biochemical models with intermediate species

    DEFF Research Database (Denmark)

    Feliu, Elisenda; Wiuf, Carsten

    2013-01-01

    Mathematical models are increasingly being used to understand complex biochemical systems, to analyse experimental data and make predictions about unobserved quantities. However, we rarely know how robust our conclusions are with respect to the choice and uncertainties of the model. Using algebraic......-state concentrations of the species in the core model, after suitable matching of parameters. Importantly, our results provide guidelines to the modeller in choosing between models and in distinguishing their properties. Further, our work provides a formal way of comparing models that share a common skeleton....

  15. Radiation treatment of drugs, biochemicals and vaccines

    International Nuclear Information System (INIS)

    Nordheim, W.; Braeuniger, S.; Kirsch, B.; Kotowski, H.; Teupel, D.

    1984-12-01

    The concise and tabulated review reports experimental results on the effects of radiation treatment on drugs, vaccines, biochemicals and adjuvants including enzymes as well. Irradiation was mostly performed by γ-radiation using 60 Co and to a lesser extent by 137 Cs, 182 Ta, X-rays and accelerators. Ionizing radiation proved to be a useful tool for sterilization and inactivation in producing drugs, vaccines, and bioactive agents and will contribute to realize procedures difficultly solvable as to engineering and economy, respectively. 124 refs

  16. Espacio biológico y prótesis fija: Del concepto clásico a la aplicación tecnológica

    OpenAIRE

    Matta Valdivieso, Edwin; Facultad de Estomatología. Universidad Peruana Cayetano Heredia. Lima,; Alarcon Palacios, Marco; Facultad de Estomatología. Universidad Peruana Cayetano Heredia. Lima,; Matta Morales, Carlos; Facultad de Estomatología. Universidad Peruana Cayetano Heredia. Lima,

    2014-01-01

    Se denomina espacio biológico a la unión dentogingival que está constituida por el epitelio de unión y el tejido conectivo de inserción de la encía. Cuando se habla de espacio biológico no sólo se debe pensar en la longitud de la inserción gingival, sino que se debe relacionar con el grosor de la encía, el biotipo periodontal y la profundidad del surco gingival. Una vez invadida la unión dentogingival, el tipo de manifestación clínica que se produce va a ser distinta según los casos debido a ...

  17. Utilização de carvão ativado biológico para o tratamento de água para consumo humano

    OpenAIRE

    Westphalen,Ana Paula Campos; Corção,Gertrudes; Benetti,Antônio Domingues

    2016-01-01

    RESUMO: Este artigo de revisão aborda o uso do carvão ativado biológico no tratamento das águas para consumo humano. O tratamento biológico tem aplicação na redução da instabilidade da água potável causada por matéria orgânica biodegradável e compostos inorgânicos reduzidos presentes em baixas concentrações. A instabilidade tem efeitos prejudiciais à qualidade da água potável, tais como o crescimento de bactérias e a formação de biofilmes na rede de distribuição. O artigo discute as causas e ...

  18. Manual práctico de reproducción masiva de Amblyseius californicus, usado en el control biológico de Tetranychus urticae

    OpenAIRE

    Colcha Rovalino, Segundo Efraín

    2013-01-01

    El Manual práctico de reproducción masiva de (Amblyseius californicus), usado en el control biológico de (Tetranychus urticae), brindará una alternativa válida y efectiva para el control del ácaros plaga, explicando paso a paso todos los procesos que conllevan a obtener la reproducción del ácaro benéfico hasta su liberación y control en el campo, y así garantizar el éxito, sin pérdidas de cosecha por la implementación de este método biológico, reemplazando al método químico que a la ves caus...

  19. Oscilações entre o reducionismo e o fisicalismo não-redutivo no naturalismo biológico de John Searle

    Directory of Open Access Journals (Sweden)

    Tárik de Athayde Prata

    2013-08-01

    Full Text Available O artigo examina a concepção de Searle sobre a redução da consciência (em especial a sua teoria acerca de seus poderes causais, que se mostra obscura e incoerente. Porém, essa incoerência não é inevitável, pois o naturalismo biológico possui elementos que permitiriam a articulação de uma teoria mais clara a respeito das capacidades causais. O exame da teoria de Pereboom e Kornblith possibilita entender por que a afirmação de identidade das capacidades causais leva a um reducionismo. Essa teoria aponta um caminho promissor para que se possa aprimorar o naturalismo biológico.

  20. Controle biológico de Botrytis cinerea em pós-colheita de morango (Fragaria x ananassa) por linhagem Streptomyces araujoniae sp. nov.

    OpenAIRE

    Leonardo José da Silva

    2014-01-01

    A produção brasileira de alimentos cresce em ritmo vertiginoso, havendo previsão de expansão nos próximos anos. Nota-se, porém, que o modelo atual empregado ao controle de doenças e pragas agrícolas, tem causado diversos problemas de ordem ambiental, social e econômica. Uma alternativa à redução de tais impactos, tem sido a implementação do controle biológico no modelo de manejo. Neste trabalho, avaliou-se o controle biológico do fungo fitopatogênico Botrytis cinerea por compostos produzidos ...

  1. W2X and W3X-L: Cost-Effective Approximations to W2 and W4 with kJ mol(-1) Accuracy.

    Science.gov (United States)

    Chan, Bun; Radom, Leo

    2015-05-12

    We have formulated the W2X and W3X-L protocols as cost-effective alternatives to W2 and W3/W4, respectively, and to supplement our previously developed set of W1X and W3X procedures. The W2X procedure provides an accurate approximation to the all-electron scalar-relativistic CCSD(T)/CBS energy, with a mean absolute deviation (MAD) of 0.6 kJ mol(-1) from benchmark energies provided by the CCSD(T) component in the W4 protocol. Such a performance is comparable to that of W2w (0.5 kJ mol(-1)) but comes at a significantly lower cost. Comparison of computational requirements shows that W2X should be applicable to systems that can be treated by the W1w method. Thus, W2X provides an accurate means for the treatment of medium-sized systems such as naphthalene. For the calculation of post-CCSD(T) effects, we propose a slight modification to the method used in our previously devised W3X procedure. Our new W3-type protocol (W3X-L) combines this new post-CCSD(T) treatment with our new W2X procedure. It has an MAD from benchmark values of 0.8 kJ mol(-1) for the W4-11 set, which is comparable to that for the computationally more demanding W3.2 method (0.6 kJ mol(-1)). However, the use of the even relatively modest post-CCSD(T) calculations in W3X-L still represents a computational bottleneck, and this currently restricts its application to systems up to the size of benzene with our current computing resources.

  2. Establishment of a clonal bank of Caesalpinia spinosa (Mol.) O. Kuntz by selection of plus trees and grafting

    OpenAIRE

    Jenny E Nuñez; Elisa Quiala; Manuel de Feria; Saúl Mestanza; Rafael Gómez-Kosky; Franklin R Cuadrado; Michel Leiva-Mora

    2017-01-01

    In Ecuador, plant propagation techniques are not available commercially to establish clonal banks of Caesalpinia spinosa (Mol.) O. Kuntz (guarango) plus trees, which limits the development of in vitro propagation protocols of this specie. The objective of the present work was to establish a clonal bank of C. spinosa by selecting plus trees and grafting. Guarango trees belonging to the province of Chimborazo, Guano canton were selected based on total height, height at the beginning of the crow...

  3. El pelo como matriz biológica alternativa y su uso en la determinación de la exposición intraútero a drogas ilícitas y fármacos

    OpenAIRE

    Lendoiro Belío, Elena

    2016-01-01

    El uso de matrices biológicas alternativas en el ámbito de la Toxicología Clínica y Forense se ha incrementado de forma significativa en los últimos años, ay que complementan la información obtenida por las matrices biológicas tradicionales. El pelo es una matriz biológica alternativa muy útil debido a su amplia ventana de detección, de meses o incluso años, que permite realizar el estudio retrospectivo del consumo y establecer el perfil cronológico del mismo. Sin embargo, para su análisis to...

  4. Structural homogeneity of photorefractive LiNbO3 crystals doped with 0.03-4.5 mol % of ZnO

    Science.gov (United States)

    Sidorov, N. V.; Palatnikov, M. N.; Teplyakova, N. A.; Gabain, A. A.; Efremov, I. N.

    2016-04-01

    Using the electronic spectroscopy method, the laser-conoscopy method, and the Raman light-scattering method, we have studied the structural homogeneity of LiNbO3 crystals doped with 0.03-4.5 mol % of ZnO. We have found that, as the laser radiation power is increased to 90 mW, the conoscopic patterns of crystals show additional distortions, which are attributed to the manifestation of the photorefractive effect. For the LiNbO3 crystal doped with 4.5 mol % of ZnO, in which the photorefractive effect is low, we have revealed a considerable shift (compared to the remaining crystals) of the optical absorption edge toward the shortwavelength range, which indicates a high structural homogeneity of this crystal. We have shown that, in the LiNbO3 crystal doped by 0.05 mol % ZnO, due to the displacement of NbLi and Li□ structural defects by Zn2+ cations, the crystal structure is ordered and, simultaneously, the number of defects with localized electrons decreases.

  5. Isolamento , caracterização parcial e atividades biológicas da lectina de entrecasca de aroeira(Schinus terebinthifolius Raddi.)

    OpenAIRE

    Mary Alves Viana, Ana

    2002-01-01

    Infusão de entrecasca de Schinus terebinthifolius Raddi., família Anacardiaceae (aroeira), tem sido comumente usada em medicina popular. Lectinas constituem um grupo heterogêneo de proteínas capaz de reconhecer e reversivelmente ligar a carboidratos. O objetivo deste trabalho foi isolar a lectina de entrecasca de S. terebinthifolius e avaliar suas propriedades biológicas. Extrato obtido do pó de entrecasca foi submetido a ensaios de atividade hemaglutinante (AH) usando eritr...

  6. Estudio de aislados no patogénicos de Fusarium oxysporum para el control biológico de patógenos fúngicos oportunistas

    OpenAIRE

    Arroyo Gallardo, Víctor

    2015-01-01

    Los hongos oportunistas afectan numerosas especies vegetales tanto en los cultivos como en productos almacenados. Este tipo de enfermedades son muy difíciles de combatir mediante el empleo de fitosanitarios. La aplicación de los mismos se encuentra muy limitada y es especialmente importante en productos destinados a consumo. Por ello, en el presente estudio planteamos el control biológico como alternativa. Para conseguir este objetivo, trabajamos con siete aislados no patogénicos de Fus...

  7. Tratamentos alternativos em apicultura biológica. Aplicação em Trás-os-Montes e Alto Douro

    OpenAIRE

    Vilas-Boas, Miguel

    2005-01-01

    A utilização de pesticidas sintéticos no combate a Varroa apresenta alguns inconvenientes, destacando-se a acumulação de resíduos, a decréscimo na eficiência do tratamento devido a ocorrência de resistência provocada pelo uso continuado e a impossibilidade da sua utilização em apicultura no modo de produção biológica (MPB).

  8. Parâmetros biológicos e tabela de vida de fertilidade de Neopamera bilobata (Hemiptera: Rhyparochromidae em morangueiro

    Directory of Open Access Journals (Sweden)

    Taciana Melissa de Azevedo Kuhn

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar os parâmetros biológicos e determinar a tabela de vida de fertilidade de Neopamera bilobata (Hemiptera: Rhyparochromidae em morangueiro. O experimento foi realizado no Laboratório de Entomologia da Embrapa Uva e Vinho, em Bento Gonçalves, RS, em câmara climatizada (a 23±1°C, 70±10% UR e fotófase de 12 horas. Utilizaram-se folíolos, flores, frutos verdes e maduros de morangueiro 'Aromas' como alimento para os percevejos. O desenvolvimento das fases imaturas e os parâmetros biológicos dos adultos foram monitorados diariamente. O percevejo N. bilobata não completou o ciclo biológico em folíolos e flores de morangueiro. Em frutos maduros e verdes, o tempo de desenvolvimento da ninfa ao adulto foi de 32,8±9,12 e 36,7±6,80 dias, com viabilidade de 27,3 e 51%, respectivamente. O período de oviposição foi de 34,7±21,2 dias, com 319,1±262,7 ovos em frutos maduros, e de 43,9±18,3 dias, com 318,2±144,7 ovos em frutos verdes. Neopamera bilobata completa seu ciclo biológico na cultura do morangueiro. Frutos verdes de morangueiro são mais adequados ao desenvolvimento deste inseto do que frutos maduros.

  9. Histologia do fígado de tilápia do Nilo ( Oreochromis niloticus alimentados com dietas contendo silagem biológica de pescado

    Directory of Open Access Journals (Sweden)

    Claucia A. Honorato

    2014-12-01

    Full Text Available Resumo: Este estudo teve como objetivo avaliar as possíveis alterações histopatológicas hepática de tilápias do Nilo alimentadas com dietas contendo silagem biológica de pescado com diferentes concentrações protéicas. Foram utilizados 180 juvenis alimentados com dietas contendo três níveis de proteína (20, 24 and 28% PB, e duas proporções de silagem biológica (¼ e ½ durante 75 dias. Os fragementos de fígado foram fixados em Bouin e inclusos em Histosec®. Posteriorente foram cortados em microtomo com espessura de 2 a 5μm. O método utilizado para coloração foi hematoxilina/eosina e PAS. Os cortes histológicos foram analisados em microscopio de luz. O desarranjo na morfologia do fígado dos peixes alimentados com silagem biológica foi influenciado pelos altos níveis protéicos, e pelo aumento ½ de proporções de proteínas de origem animal das dietas. Foi observado que a variação dos hepatócitos está diretamente ligada com o tipo da dieta fornecida para os peixes. Nos peixes alimentados com as dietas contendo 28% PB, o fígado apresentou desarranjo da estrutura cordonal dos hepatócitos, pontos de necrose e deslocamento do núcleo para periferia. Níveis elevados de silagem biológica de pescado provoca alterações deletérias no fígado. O nível de proteína adequado para manter o desenvolvimento associado à saúde do peixe é de 24%PB.

  10. Insatisfação corporal e maturação biológica em atletas do sexo masculino

    Directory of Open Access Journals (Sweden)

    Leonardo de Sousa Fortes

    2013-06-01

    Full Text Available O objetivo do presente estudo foi investigar a influência da maturação biológica na insatisfação corporal (IC de jovens atletas do sexo masculino. Participaram da pesquisa 464 esportistas. A IC foi mensurada pelo Body Shape Questionnaire (BSQ. A maturidade biológica foi estimada pela maturação somática. Peso, estatura e dobras cutâneas foram aferidos para calcular o índice de massa corporal (IMC e estimar o percentual de gordura, respectivamente. Foram conduzidos testes estatísticos em "software" especializado para analisar os dados. Os resultados não evidenciaram diferenças estatisticamente significativas para as pontuações do BSQ segundo classificações de maturação somática. Por outro lado, identificaram-se diferenças de IMC e percentual de gordura entre estágios maturacionais (p < 0,05. Deste modo, concluiu-se que a IC dos jovens atletas não sofreu influência da maturação biológica.

  11. A determinação biológica dos transtornos mentais: uma discussão a partir de teses neurocientíficas recentes

    Directory of Open Access Journals (Sweden)

    Luna Rodrigues Freitas-Silva

    2016-01-01

    Full Text Available Resumo: A compreensão dos processos de formação dos transtornos mentais vem se mostrando desafiadora desde a fundação do campo psiquiátrico. O desenvolvimento das neurociências proporcionou novo fôlego à expectativa de encontrar estritamente no funcionamento biológico a explicação para o surgimento dos transtornos mentais. No entanto, tal objetivo não vem sendo alcançado com a esperada facilidade, de modo que novas hipóteses começam a se destacar nas pesquisas neurocientíficas. Neste artigo, identificamos as noções de epigenética, neurodesenvolvimento e plasticidade como os principais indicativos de um novo modo de compreender a biologia dos fenômenos mentais. A complexidade genética, o papel formativo do ambiente e as variações que caracterizam a vulnerabilidade implicam importantes modificações nas principais teses sobre a determinação biológica dos transtornos mentais, sugerindo uma reconfiguração dos limites entre o "social" e o "biológico" nas pesquisas em neurociências.

  12. Uso y verificación con indicadores biológicos en esterilizadores de cirujanos dentistas de San Luis Potosí, México

    Directory of Open Access Journals (Sweden)

    Patiño-Marín Nuria

    2001-01-01

    Full Text Available Objetivo. Conocer el uso y verificar los ciclos de esterilización con indicadores biológicos en los equipos utilizados por cirujanos dentistas de la Facultad de Estomatología de la Universidad Autónoma de San Luis Potosí (UASLP y del Colegio Dental Potosino. Material y métodos. Estudio transversal hecho en 1999-2000. El 65% (n=130 de los odontólogos participaron con un esterilizador, la verificación se realizó por indicadores que contenían esporas de Bacillus subtilis y de Bacillus stearothermophilus. Resultados. Participaron 30 autoclaves y 100 esterilizadores de calor seco, 23 de ellos (17.7% presentaron crecimiento bacteriano; el 16.1% (n=21 de los participantes utilizan los indicadores biológicos como verificador. Los dos métodos de esterilización presentaron crecimiento bacteriano con frecuencias similares (p=>0.66. Conclusiones. Pocos cirujanos dentistas verifican su esterilizador con indicadores biológicos en los equipos que presentaron crecimiento bacteriano, sus fallas se encontraron en el proceso de esterilización. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html

  13. Contribuições de componentes da licenciatura em Ciências Biológicas na constituição do educador ambiental

    Directory of Open Access Journals (Sweden)

    Gabriela de Vargas de Rosa

    2015-12-01

    Full Text Available A educação ambiental é uma construção histórica de valores e responsabilidades, num processo de mudanças de atitudes quanto a relação ser humano e natureza, considerando a vida em sociedade. O objetivo do trabalho é verificar a contribuição da formação profissional na Licenciatura em Ciências Biológicas para a constituição do educador ambiental, numa perspectiva interdisciplinar do conhecimento. Para tanto, foram realizadas as análises das ementas e referências dos componentes curriculares Ecologia, Prática de Ensino I: Fundamentos teóricos e práticos em ciências e Prática de ensino VI: Prática de Pesquisa Biológica inscritos no Projeto Pedagógico do Curso de Ciências Biológicas da Universidade Regional do Noroeste do Estado do Rio Grande do Sul (UNIJUI, implantado em 2009. As discussões propiciadas a partir deste conhecimento, em sala de aula, proporcionam espaços de reflexão sobre a responsabilidade individual e coletiva na mudança de atitude quanto a relação com o ambiente.

  14. The Drosophila genes CG14593 and CG30106 code for G-protein-coupled receptors specifically activated by the neuropeptides CCHamide-1 and CCHamide-2

    DEFF Research Database (Denmark)

    Hansen, Karina K; Hauser, Frank; Williamson, Michael

    2011-01-01

    Recently, a novel neuropeptide, CCHamide, was discovered in the silkworm Bombyx mori (L. Roller et al., Insect Biochem. Mol. Biol. 38 (2008) 1147-1157). We have now found that all insects with a sequenced genome have two genes, each coding for a different CCHamide, CCHamide-1 and -2. We have also...... flea Daphnia pulex (Crustacea) and the tick Ixodes scapularis (Chelicerata)....

  15. Women with an HbA1c of 41-49 mmol/mol (5.9-6.6%): a higher risk subgroup that may benefit from early pregnancy intervention.

    Science.gov (United States)

    Rowan, J A; Budden, A; Ivanova, V; Hughes, R C; Sadler, L C

    2016-01-01

    To examine whether women with an HbA1c of 41-49 mmol/mol (5.9-6.6%) at diagnosis of gestational diabetes are higher risk than women with an HbA1c of mol (5.9%) and whether pregnancy outcomes are improved if treated at mol (5.9-6.6%) were divided into those seen mol (5.9%) were labelled Other GDM (n = 661). The Early and Later groups, compared with Other GDM, had more Polynesian and fewer (non-Indian) Asian women, higher BMI and more required medication (P mol (5.9-6.7%) identifies a higher-risk group of women with gestational diabetes. Overall, our data support early treatment of women with an HbA1c ≥ 41 mmol/mol (5.9%). © 2015 The Authors. Diabetic Medicine © 2015 Diabetes UK.

  16. Nutritional and biochemical therapies for neurodegenerative diseases

    Directory of Open Access Journals (Sweden)

    George E. Barreto

    2015-04-01

    Full Text Available Neurodegenerative diseases such as Parkinson’s disease, Huntington and Alzheimer’s disease are characterized by neuronal death and loss in different areas of the brain. Downstream signaling mechanisms associated to cellular death/survival are altered, where mitochondrial damage and inflammation, dysfunctional autophagy process, and accumulation of toxins proteins play a central role in the pathogenesis of these diseases. The disabling effects of these diseases on health system are high and greatly affect the health and daily lifestyle of patients. In this context, pharmacological and non-pharmacological therapies, which are used in palliative and preventive treatments, have been widely assessed in human patients, as well as animal and cellular models in the last decades. However, the genetics and epigenetics factors of any disease can cause different paths in its progression. Nutritional and biochemical therapy approaches by activation or manipulation of different transcription factors such as Nrf2, PPARα, CREB and TEFB in animal and cellular models have shown protective effects against neurodegeneration. Some of these therapies include caloric restriction diet, use of glutathione precursors and Mediterranean diet. This work highlights the evidences of different nutritional and biochemical approaches for the treatment of neurodegenerative diseases and how novel research approaches, such as the use of systems biology, will allow a better comprehension of key processes and biological responses involved in these diseases.

  17. Biochemical processes for geothermal brine treatment

    Energy Technology Data Exchange (ETDEWEB)

    Premuzic, E.T.; Lin, M.S.; Bohenek, M.; Joshi-Tope, G.; Zhou, W.; Shelenkova, L.; Wilke, R.

    1998-08-01

    As part of the DOE Geothermal Energy Program, BNL`s Advanced Biochemical Processes for Geothermal Brines (ABPGB) project is aimed at the development of cost-efficient and environmentally acceptable technologies for the disposal of geothermal wastes. Extensive chemical studies of high and low salinity brines and precipitates have indicated that in addition to trace quantities of regulated substances, e.g., toxic metals such as arsenic and mercury, there are significant concentrations of valuable metals, including gold, silver and platinum. Further chemical and physical studies of the silica product have also shown that the produced silica is a valuable material with commercial potential. A combined biochemical and chemical technology is being developed which (1) solubilizes, separates, and removes environmentally regulated constituents in geothermal precipitates and brines, (2) generates an amorphous silica product which may be used as feedstock for the production of revenue generating materials, (3) recover economically valuable trace metals and salts. Geothermal power resources which utilize low salinity brines and use the Stretford process for hydrogen sulfide abatement generate a contaminated sulfur cake. Combined technology converts such sulfur to a commercial grade sulfur, suitable for agricultural use. The R and D activities at BNL are conducted jointly with industrial parties in an effort focused on field applications.

  18. BIOCHEMICAL PROCESSES FOR GEOTHERMAL BRINE TREATMENT

    Energy Technology Data Exchange (ETDEWEB)

    PREMUZIC,E.T.; LIN,M.S.; BOHENEK,M.; JOSHI-TOPE,G.; ZHOU,W.; SHELENKOVA,L.; WILKE,R.

    1998-09-20

    As part of the DOE Geothermal Energy Program, BNL's Advanced Biochemical Processes for Geothermal Brines (ABPGB) project is aimed at the development of cost-efficient and environmentally acceptable technologies for the disposal of geothermal wastes. Extensive chemical studies of high and low salinity brines and precipitates have indicated that in addition to trace quantities of regulated substances, e.g., toxic metals such as arsenic and mercury, there are significant concentrations of valuable metals, including gold, silver and platinum. Further chemical and physical studies of the silica product have also shown that the produced silica is a valuable material with commercial potential. A combined biochemical and chemical technology is being developed which (1) solubilizes, separates, and removes environmentally regulated constituents in geothermal precipitates and brines (2) generates an amorphous silica product which may be used as feedstock for the production of revenue generating materials, (3) recover economically valuable trace metals and salts. Geothermal power resources which utilize low salinity brines and use the Stretford process for hydrogen sulfide abatement generate a contaminated sulfur cake. Combined technology converts such sulfur to a commercial grade sulfur, suitable for agricultural use. The R and D activities at BNL are conducted jointly with industrial parties in an effort focused on field applications.

  19. Rebinding in biochemical reactions on membranes

    Science.gov (United States)

    Lawley, Sean D.; Keener, James P.

    2017-10-01

    The behavior of many biochemical processes depends crucially on molecules rapidly rebinding after dissociating. In the case of multisite protein modification, the importance of rebinding has been demonstrated both experimentally and through several recent computational studies involving stochastic spatial simulations. As rebinding stems from spatio-temporal correlations, theorists have resorted to models that explicitly include space to properly account for the effects of rebinding. However, for reactions in three space dimensions it was recently shown that well-mixed ordinary differential equation (ODE) models can incorporate rebinding by adding connections to the reaction network. The rate constants for these new connections involve the probability that a pair of molecules rapidly rebinds after dissociation. In order to study biochemical reactions on membranes, in this paper we derive an explicit formula for this rebinding probability for reactions in two space dimensions. We show that ODE models can use the formula to replicate detailed stochastic spatial simulations, and that the formula can predict ultrasensitivity for reactions involving multisite modification of membrane-bound proteins. Further, we compute a new concentration-dependent rebinding probability for reactions in three space dimensions. Our analysis predicts that rebinding plays a much larger role in reactions on membranes compared to reactions in cytoplasm.

  20. Biochemical research elucidating metabolic pathways in Pneumocystis*

    Directory of Open Access Journals (Sweden)

    Kaneshiro E.S.

    2010-12-01

    Full Text Available Advances in sequencing the Pneumocystis carinii genome have helped identify potential metabolic pathways operative in the organism. Also, data from characterizing the biochemical and physiological nature of these organisms now allow elucidation of metabolic pathways as well as pose new challenges and questions that require additional experiments. These experiments are being performed despite the difficulty in doing experiments directly on this pathogen that has yet to be subcultured indefinitely and produce mass numbers of cells in vitro. This article reviews biochemical approaches that have provided insights into several Pneumocystis metabolic pathways. It focuses on 1 S-adenosyl-L-methionine (AdoMet; SAM, which is a ubiquitous participant in numerous cellular reactions; 2 sterols: focusing on oxidosqualene cyclase that forms lanosterol in P. carinii; SAM:sterol C-24 methyltransferase that adds methyl groups at the C-24 position of the sterol side chain; and sterol 14α-demethylase that removes a methyl group at the C-14 position of the sterol nucleus; and 3 synthesis of ubiquinone homologs, which play a pivotal role in mitochondrial inner membrane and other cellular membrane electron transport.

  1. [Biochemical principles of early saturnism recognition].

    Science.gov (United States)

    Tsimakuridze, M P; Mansuradze, E A; Zurashvili, D G; Tsimakuridze, M P

    2009-03-01

    The aim of the work is to determine the major sensitive criteria of biochemical indicators that allow timely discovery of negative influence of lead on organism and assist in early diagnosis of primary stages of saturnism. The workers of Georgian typographies, performing technological processes of letterpress printing were observed. Professional groups having contact with lead aerosols (main group of 66 people) and the workers of the same typography not being in touch with the poison (control group of 24 people) were studied. It was distinguished that, protracted professional contact with lead causes moderate increase of lead, coproporphyrin and DALA in daily urine in most cases; it is more clearly evidenced in the professional groups of lead smelters and lino operators and less clearly among typesetter and printers. Upon the checkup of people, having a direct contact with lead, biochemical analysis of urine should be given a preference, especially the determination of quantitative content of lead and coproporphyrin in urine with the aim of revealing the lead carrier, which is one of the first signals for occupational lookout and medical monitoring of the similar contingent.

  2. Pulmonary biochemical alterations resulting from ozone exposure

    Energy Technology Data Exchange (ETDEWEB)

    Mustafa, M.G.; Lee, S.D.

    1976-07-01

    Metabolic response of lung tissue to ozone was studied in rats and monkeys after exposure of animals to various levels of ozone (0.1 to 0.8 ppM) for 1 to 30 days. In rats, 0.8 ppM ozone exposure resulted in a 40 to 50 percent augmentation of oxygen utilization in lung homogenate in the presence of an added substrate (e.g., succinate or 2-oxoglutarate). Activities of marker enzymes, viz. mitochondrial succinate-cytochrome c reductase; microsomal NADPH-cytochrome c reductase and cytosolic glucose-6-phosphate dehydrogenase, increased maximally (40 to 70 percent over control) after 3 to 4 days of exposure, and remained elevated throughout the 0.8 ppM ozone exposure for 30 days. In monkeys, the observations were the same except that the magnitude of biochemical changes was relatively smaller. Exposure of animals to lower levels of ozone resulted in proportionately smaller biochemical changes in the lung, and ozone effects were detectable up to the 0.2 ppM level. While 0.1 ppM ozone exposure was ineffective, dietary deficiency of vitamin E, a natural antioxidant, increased the sensitivity of rat lungs to this concentration of ozone. The results suggest that low-level ozone exposures may cause metabolic alterations in the lung, and that dietary supplementation of vitamin E may offer protection against oxidant stress.

  3. Biochemical Manifestation of HIV Lipodystrophy Syndrome.

    Science.gov (United States)

    Ihenetu, Kenneth; Mason, Darius

    2012-01-01

    Highly active anti-retroviral therapy (HAART), including protease inhibitors (PI) have led to dramatic improvements in the quality and quantity of life in patients with acquired immunodeficiency syndrome (AIDS). However, a significant number of AIDS patients on HAART develop characteristic changes in body fat redistribution referred to as lipodystrophy syndrome (LDS). Features of LDS include hypertrophy in the neck fat pad (buffalo hump), increased fat in the abdominal region (protease paunch), gynecomastia and loss of fat in the mid-face and extremities. The aim of this paper is to review the current knowledge regarding this syndrome. This article reviews the published investigations on biochemical manifestation of HIV lipodystrophy syndrome. It is estimated that approximately 64% of patients treated with PI will experience this syndrome. Biochemically, these patients have increased triglycerides (Trig), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C) and extremely low high-density lipoprotein-cholesterol (HDL-C). It is hoped that awareness of this syndrome would aid in early diagnosis and better patient management, possibly leading to a lower incidence of cardiovascular complications among these patients.

  4. Biochemical markers of neonatal myocardial dysfunction.

    Science.gov (United States)

    Almeida, Cristina Maria; Carrapato, Manuel R G; Pinto, Fernando; Pinto, Mariana; Ferreira, Sofia; Schmitt, Denise; Marinho, Luis

    2011-04-01

    Cardiac ultrasounds (US) are not always available at the bedside. Cardiac Troponin I (cTnI), CK-MB and NT-proBNP may be an alternative or complementary to influence evaluation and treatment. To determine reference ranges of biochemical markers cTnI, CK-MB and NT-proBNP in normal neonates. Cord and blood samples were collected from neonates and the above biochemical markers were determined. Ultrasounds were performed blindly. CK-MB remains constant from cord blood to the first day, declining thereafter to almost half the values (81.5 vs 52.0 U/l); cTnI increases from 0.004 to 0.058 ng/ml by 72 h falling to 0.030 by day 10; NT-proBNP peaks by 24 h (5085.5 pg/ml), subsiding to 3388.5 pg/ml by day 3, falling to 1316.0 pg/ml by day 10. CK-MB, mostly of muscle origin and reflecting labor stress or injury, is not to recommend as a measure of myocardial damage in the neonate. The rise in cTnI may be explained by a degree of myocardial involvement, albeit physiological. The initial rise and subsequent fall of NT-proBNP represents the physiological ventricular overload of transient birth adaptation.

  5. Anesthetic efficacy of combinations of 0.5 mol/L mannitol and lidocaine with epinephrine for inferior alveolar nerve blocks in patients with symptomatic irreversible pulpitis.

    Science.gov (United States)

    Kreimer, Timothy; Kiser, Russell; Reader, Al; Nusstein, John; Drum, Melissa; Beck, Mike

    2012-05-01

    The purpose of these 2 prospective, randomized, single-blind studies was to determine the anesthetic efficacy of lidocaine with epinephrine compared with a combination lidocaine with epinephrine plus 0.5 mol/L mannitol for inferior alveolar nerve (IAN) blocks in patients experiencing symptomatic irreversible pulpitis. In study one, 55 emergency patients randomly received IAN blocks by using a 3.18-mL formulation containing 63.6 mg of lidocaine with 31.8 μg epinephrine or a 5-mL formulation containing 63.6 mg of lidocaine with 31.8 μg epinephrine (3.18 mL) plus 1.82 mL of 0.5 mol/L mannitol. In study two, 51 emergency patients randomly received IAN blocks by using a 1.9-mL formulation containing 76.4 mg of lidocaine with 36 μg epinephrine or a 3-mL formulation containing 76.4 mg of lidocaine with 36 μg epinephrine (1.9 mL) plus 1.1 mL of 0.5 mol/L mannitol. Endodontic access was begun 15 minutes after the IAN block, and all patients had profound lip numbness. Success was defined as no or mild pain (visual analogue scale recordings) on endodontic access or instrumentation. The 1.9 mL of lidocaine (76.4 mg) with epinephrine plus 0.5 mol/L mannitol had a significantly (P = .04) better success rate of 39% when compared with the lidocaine formulation without mannitol (13% success rate). For mandibular posterior teeth in patients with symptomatic irreversible pulpitis, the addition of 0.5 mol/L mannitol to 1.9 mL of lidocaine (76.4 mg) with epinephrine resulted in a statistically higher success rate. However, the combination lidocaine/mannitol formulation would not result in predictable pulpal anesthesia. Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  6. Acidentes de trabalho com material biológico em hospital universitário de São Paulo

    Directory of Open Access Journals (Sweden)

    Tanyse Galon

    2008-09-01

    Full Text Available Pesquisa exploratória descritiva com o objetivo de identificar a ocorrência e as características dos acidentes de trabalho (AT com exposição a material biológico entre trabalhadores de um hospital do Estado de São Paulo. Os dados foram coletados por meio de levantamento documental dos registros de AT no hospital e posterior registro no protocolo eletrônico da Rede de Prevenção de Acidentes de Trabalho (REPAT. Os dados foram tratados com uso da estatística descritiva e apresentados em tabelas e figuras. No ano de 2006 foram notificados 94 acidentes envolvendo exposição do trabalhador a fluidos corpóreos no hospital. Considerando-se a proporcionalidade das categorias expostas, o instrumentador cirúrgico (18,75%, o auxiliar de enfermagem (7,78% e o biologista (4,76% foram as categorias mais vitimadas. A agulha hipodérmica foi o agente causador de lesão em 71,62% dos casos. Os AT ocorreram em maior freqüência na administração de medicamentos (32,97% e na coleta de sangue (15,95%. Constatou-se a existência de dados parciais quanto ao uso de Equipamentos de Proteção Individual e a situação vacinal dos trabalhadores. Os resultados revelaram indicadores importantes das características dos acidentes e as lacunas de registros extremamente importantes para a elaboração de um programa preventivo eficaz para minimizar a ocorrência dos acidentes.

  7. Maduración biológica, fuerza y potencia muscular en la brazada de crol

    Directory of Open Access Journals (Sweden)

    Richard R. Casanova Machek

    2017-06-01

    Full Text Available Se trata de una investigación de campo, de tipo correlacional que buscó determinar la relación entre la maduración biológica y la potencia muscular de los miembros superiores en nadadores de categorías menores. Se evaluaron 23 niños(as edades comprendidas entre los 10 y los 13 años. La muestra se caracterizó a través de mediciones antropométricas: estatura, masa corporal, envergadura, composición corporal y superficie corporal, maduración sexual (Tanner, 1975. Se aplicó Test de Wingate (laboratorio y piscina según Dotan y Bar-Or (1983 y Morouco (2009 para miembros superiores para estimar potencia máxima, potencia relativa, potencia promedio, índice de fatiga. Los datos se presentan en medidas de tendencia central y dispersión; se calcularon las diferencias de un factor y se estimaron las correlaciones con la técnica Pearson y Spearman. Los resultados indican que el entrenamiento fuera del agua influyó en el rendimiento dentro del agua. El tamaño de las dimensiones corporales y el número de horas de entrenamiento tiene incidencia sobre la potencia producida por los nadadores, con diferencias notables a partir de los 10 años. Las dimensiones corporales y la mayor frecuencia de entrenamiento semanal generan valores altos de potencia muscular. Se recomienda realizar evaluaciones periódicas de fuerza y potencia, crear y mantener actualizado el perfil antropométrico, aplicar cuestionario de autoevaluación de caracteres de maduración sexual y ajustar los periodos de duración de las horas de entrenamiento, en tanto se incrementen las edades.

  8. Actividad contra Leishmania sp. (Kinetoplastida: Trypanosomatidae de plantas en una Reserva Biológica de Costa Rica

    Directory of Open Access Journals (Sweden)

    Misael Chinchilla-Carmona

    2014-09-01

    Full Text Available La leishmaniosis es una enfermedad muy importante para el ser humano pero su tratamiento es bastante difícil. Por esta razón muchos investigadores han venido buscando plantas que contengan componentes químicos activos contra esta parasitosis. En Costa Rica no se tienen estudios al respecto y por eso el objetivo de este estudio fue la búsqueda de componentes activos contra Leishmania sp. en plantas de Costa Rica; 67 especies de la Reserva Biológica Alberto Manuel Brenes (REBAMB fueron seleccionadas para realizar este trabajo. Para ello se prepararon extractos crudos hidro-alcohólicos de material fresco o desecado de raíz, tallo, hojas maduras o tiernas, flores y frutos inmaduros o maduros. Usando pruebas presuntivas y luego específicas, se analizó el efecto de tales extractos sobre una cepa de Leishmania (OCR. Se consideraron plantas promisorias solamente aquellas en que al menos una de sus partes presentara un CI50<100µg/mL. Las plantas seleccionadas fueron: Bocconia frutescens, Clematis dioica,Cordia megalantha, Eugenia austin-smithii, Guarea bullata, Guateria tonduzii, Mikania holwayana, Nectandra membranacea,Neurolaena lobata, Persea povedae, Piper auritum, Rollinia pittieri, Solanum arboreum, Tetrorchidium euryphyllum, Witheringia solanacea y Zanthoxylum juniperinum. Existió una ligera tendencia de positividad mayor para los extractos frescos y la actividad se presentó en una y hasta más de cuatro partes de la planta. La mayoría de los extractos activos no fueron tóxicos. Se discute la importancia de estos nuevos hallazgos, en relación con el nuevo conocimiento científico y su proyección en el tratamiento de la leishmaniosis.

  9. Evolución biológica y cultural: una mirada a la tecnología

    Directory of Open Access Journals (Sweden)

    Angela Landínez

    2010-10-01

    Full Text Available El artículo presenta una reflexión sobre la tecnología; para esto se establece una correlación entre la evolución biológica y la cultural. Además, se profundiza en el concepto de la técnica, sobre la cual se presenta una corta descripción de subdesarrollo histórico. También, se plantea la problemática de la biotecnología, la cual podría llegar a tener un fin meramente utilitario, por lo que está llamada a conservar la intencionalidad de su enfoque en el interior de una cultura que aún no hatenido suficiente tiempo para discutir las posibles implicaciones humanas, tecnológicas, ambientales y éticas de tan acelerado desarrollo. En estaperspectiva, se presenta a la bioética como un importante espacio de diálogo frente a la ambigüedad del desarrollo tecnológico. Finalmente, se sostiene que el problema en relación con el fenómeno tecnológico no está en la tecnología per se, sino en la difusión de una mentalidad tecnócrata que hace que la técnica pierda su carácter de medio para convertirse en fin; por lo cual se sugiere reinventar una forma de relacionarse con la racionalidad tecnológica.

  10. Revisión de los aspectos biológicos y diagnósticos del Trypanosoma (Herpetosoma rangeli

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    Cuba Cuba César A.

    1998-01-01

    Full Text Available Esta revisión tiene tres objetivos básicos: a estimular aún más la investigación de esta prevalente infección humana. b examinar el arsenal de técnicas diagnósticas disponíbles al momento y, las nuevas pruebas descritas recientemente. c enfatizar el significado que tiene, el parasitismo por el Trypanosoma (Herpetosoma rangeli, en las áreas endémicas de la Enfermedad de Chagas distribuidas en las Américas Central y del Sur. Trypanosoma rangeli y Trypanosoma cruzi son parásitos que circulan superponiéndose en muchas áreas de Latinoamérica utilizando prácticamente los mismos triatominos vectores. Una vasta gama de especies de mamíferos han sido encontradas infectadas naturalmente con T. rangeli en diversos países. Se revisa la biología del parasitismo y el ciclo biológico del tripanosoma haciendo énfasis en este último. Infecciones crónicas por T. rangeli en el hombre pueden, serológicamente, ser confundidas con las del T. cruzi. Ambas especies presentan antígenos comunes que provocan las conocidas reacciones serológicas cruzadas. Desafortunadamente, no conocemos la real distribución de las infecciones por el T. rangeli en la mayoría de las áreas mencionadas. Nuevos estudios epidemiológicos son necesarios, para examinar el problema de las infecciones humanas mixtas, por estos tripanosomas.

  11. Manufactura biológica e inteligente: atributos de la vida aplicados al desarrollo tecnológico

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    Claudia Nelcy Jiménez Hernández

    2009-05-01

    Full Text Available Los entornos altamente dinámicos caracterizados por el predominio del conocimiento como motor del desarrollo hacen que sea necesario abordar problemáticas específicas a través de enfoques multidisciplinarios. En este orden de ideas, mediante la imi- tación del comportamiento de los seres vivos se han generado técnicas y herramientas aplicadas en contextos como los tecnoló- gicos y productivos para incrementar la eficiencia y la capacidad de respuesta. Este artículo presenta los resultados de una in- vestigación efectuada mediante la aplicación de técnicas cienciométricas y de vigilancia tecnológica, con el fin de evidenciar el impacto que los conceptos y fundamentos de las ciencias de la vida han tenido sobre el desarrollo tecnológico y su gestión. De esta manera se encontró que dicho impacto se ha reflejado principalmente en la generación de conceptos y aplicaciones a temas como la manufactura inteligente, los sistemas biológicos de fabricación, la manufactura holónica y biónica, otorgando a- tributos de adaptación, autoaprendizaje, flexibilidad y capacidad de evolución a los procesos de fabricación y de manejo de in- formación, lo cual permite concluir que la gestión del factor tecnológico se ha fortalecido con base en campos como el bio- lógico, con repercusiones directas en los procesos productivos.

  12. Biodigestores: factores químicos, físicos y biológicos relacionados con su productividad

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    Olga Rivas Solano

    2010-10-01

    Full Text Available Los biodigestores son sistemas diseñados para optimizar la producción de biogás por medio de desechos orgánicos, lo que permite obtener energía limpia, renovable y de bajo costo. En el ITCR se efectuó, durante el año 2008, una actividad de fortalecimiento de la investigación con el propósito de desarrollar un sistema electrónico e inalámbrico de control y protección para la producción de biogás. Como parte de los objetivos específicos se planteó investigar sobre los métodos que existen para acelerar la descomposición de la materia prima durante la producción del gas. Para elaborar el presente artículo se hizo una revisión con el fin de establecer los parámetros matemáticos implicados en la estimación de la productividad de un biodigestor. Posteriormente, se indagó la influencia de los factores químicos en la producción del mismo, entre ellos la composición de los sustratos y sus combinaciones, la adición de grasas y la presencia de inhibidores. Luego se estudió el efecto de factores físicos como la temperatura, la remoción de los sólidos y de las proteínas de los lodos y la separación de las fases de la digestión anaerobia de la materia orgánica. Finalmente, se investigó el papel de factores biológicos como la adición de bacterias termofílicas y la importancia de los metanógenos.

  13. Managing and delivering of 3D geo data across institutions has a web based solution - intermediate results of the project GeoMol.

    Science.gov (United States)

    Gietzel, Jan; Schaeben, Helmut; Gabriel, Paul

    2014-05-01

    The increasing relevance of geological information for policy and economy at transnational level has recently been recognized by the European Commission, who has called for harmonized information related to reserves and resources in the EU Member States. GeoMol's transnational approach responds to that, providing consistent and seamless 3D geological information of the Alpine Foreland Basins based on harmonized data and agreed methodologies. However, until recently no adequate tool existed to ensure full interoperability among the involved GSOs and to distribute the multi-dimensional information of a transnational project facing diverse data policy, data base systems and software solutions. In recent years (open) standards describing 2D spatial data have been developed and implemented in different software systems including production environments for 2D spatial data (like regular 2D-GI-Systems). Easy yet secured access to the data is of upmost importance and thus priority for any spatial data infrastructure. To overcome limitations conditioned by highly sophisticated and platform dependent geo modeling software packages functionalities of a web portals can be utilized. Thus, combining a web portal with a "check-in-check-out" system allows distributed organized editing of data and models but requires standards for the exchange of 3D geological information to ensure interoperability. Another major concern is the management of large models and the ability of 3D tiling into spatially restricted models with refined resolution, especially when creating countrywide models . Using GST ("Geosciences in Space and Time") developed initially at TU Bergakademie Freiberg and continuously extended by the company GiGa infosystems, incorporating these key issues and based on an object-relational data model, it is possible to check out parts or whole models for edits and check in again after modification. GST is the core of GeoMol's web-based collaborative environment designed to

  14. Molecular Weight and Association of Asphaltenes: a Critical Review Masse moléculaire et association des asphaltènes : une revue critique

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    Speight J. G.

    2006-11-01

    Full Text Available The determination of asphaltene molecular weights is complicated by the tendency of asphaltene molecules to associate with each other and with other petroleum constituents, and reported molecular weights vary from 900 to 300 000. This paper reviews the methods (vapor pressure osmometry, size exclusion chromatography, ultrafiltration, ultracentrifugation, viscosity, small angle X-ray scattering, infrared spectroscopy, solubilization, and interfacial tension that have been used to estimate asphaltene molecular weights and to probe association phenomena. It is concluded that asphaltene fractions from typical crudes have a number average molecular weight of 1 200-2 700 and a molecular weight range of 1,000-10,000 or higher. Intermolecular association phenomena are primarily responsible for observed molecular weights up to and in excess of 100,000 but detailed mechanisms of the intermolecular associations are not well understood. Certain observations suggest that asphaltene molecules are associated in reversedmicelles and that asphaltenes interact selectively with resins although the evidence on these points is subject to alternate interpretations. H-bond interactions between asphaltenes and resins have been demonstrated. La détermination de la masse moléculaire des asphaltènes est difficile à cause de la tendance qu'ont les molécules d'asphaltènes à s'associer les unes aux autres et avec d'autres constituants des pétroles. Ces masses moléculaires varient de 900 à 300 000. Cet article passe en revue les méthodes (osmométrie par tension de vapeur, chromatographie d'exclusion stérique, ultrafiltration, ultracentrifugation, viscosité, diffusion centrale des rayons X, spectroscopie infra-rouge, solubilisation et tension interfaciale qui ont été utilisées pour estimer les masses moléculaires des asphaltènes et pour étudier les phénomènes d'association. On conclut que les asphaltènes extraits de bruts types ont des masses mol

  15. Effect of Ca and Li additions on densification and electrical conductivity of 10 mol% gadolinia-doped ceria prepared by the coprecipitation technique; Efeito de adicoes de litio e calcio na densificacao e na condutividade eletrica da ceria-10% mol gadolinia preparada pela tecnica de co-precipitacao

    Energy Technology Data Exchange (ETDEWEB)

    Porfirio, T.C.

    2010-07-01

    Ceria containing rare-earth ceramics are potential candidates for application in intermediate-temperature solid oxide fuel cells. One of the main problems related to these ceramic materials is their relatively low sinterability. In this work, the effects of Ca and Li additions on densification and electrical conductivity of 10 mol% gadolinia-doped ceria was investigated. Ceramic compositions containing 1.5 mol% Ca or Li were prepared by the oxalate coprecipitation technique. Results of sintered density and electrical conductivity were compared to those of ceramic samples obtained by solid state reactions showing the effects of the synthesis method on densification and total electrical conductivity of the sintered materials. (author)

  16. Retraction: Kartelija G, Nedeljković M, Radenović L. Photosensitive neurons in mollusks, Arch Biol Sci. 2005; 57(4:247-58, DOI:10.2298/ABS0504247K

    Directory of Open Access Journals (Sweden)

    Editorial

    2015-01-01

    Full Text Available This is a notice of retraction of the article: Photosensitive neurons in mollusks, published in the Archives of Biological Sciences in 2005, Vol. 57, Issue 4. The editorial board has been informed that this paper plagiarizes an earlier paper: Kartelija G, Nedeljkovic M, Radenovic L. Photosensitive neurons in mollusks. Comp Biochem Physiol A Mol Integr Physiol. 2003, 134(3:483-495. This claim is correct and the entire paper is a verbatim copy of the earlier one. After confirmation of this fact, the editorial board of the Archives of Biological Sciences has decided to retract the paper immediately. We apologize to the readers of the journal that it took so many years to notice this error and to retract the paper. We request readers of the journal to directly get in touch with the editorial office and the editors of the journal for similar cases in the future, so that they can be handled promptly. Link to the retracted article 10.2298/ABS0504247K

  17. THE INVESTIGATION OF BIOCHEMICAL CONTENT OF Elaeagnus angustifolia

    OpenAIRE

    yıldırım, Işıl

    2018-01-01

    AbstractStudies about herbal products are increasing every day due to their rich biochemical content. Elaeagnus angustofolia is one of the best known plant species to have a strong biochemical substance spectrum. This work was performed to identify the some biochemical content of Eleagnus angustofolia. In this study, vitamins A, E, and C, total sugar content, inverted sugar content, cellulose content, amount of total protein, and fatty acid properties were studied. Our investigations revealed...

  18. BioNessie - a grid enabled biochemical networks simulation environment

    OpenAIRE

    Liu, X.; Jiang, J.; Ajayi, O.; Gu, X.; Gilbert, D.; Sinnott, R.O.

    2008-01-01

    The simulation of biochemical networks provides insight and understanding about the underlying biochemical processes and pathways used by cells and organisms. BioNessie is a biochemical network simulator which has been developed at the University of Glasgow. This paper describes the simulator and focuses in particular on how it has been extended to benefit from a wide variety of high performance compute resources across the UK through Grid technologies to support larger scale simulations.

  19. Biochemical Measurements of the Human Stress Response

    Science.gov (United States)

    1984-03-01

    8217 biochemical stress response. Dr Thomas Longridge was the project scientist and Dr Joe De Maio was the task scientist. This research was conducted by the...under condition A described in next paragraph. Second, DOPAC, MHPG, HVA, 5-HIAA, and VMA were determined by a modified method of Joseph, Kadam , and...n tt Ln 0 0 vJ 0 (N ’lw %.D w 0 0) to CI la, co 0 (r) Ch 𔃺I I 0 𔃺 0 0 0 (’) v 01-4 V~ 4 Hn qqJ Ln NOD & OD N r- ai r, m 0 ~ $4 c4 H 0 OI H r) (n t

  20. Prions: the danger of biochemical weapons

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    Eric Almeida Xavier

    2014-09-01

    Full Text Available The knowledge of biotechnology increases the risk of using biochemical weapons for mass destruction. Prions are unprecedented infectious pathogens that cause a group of fatal neurodegenerative diseases by a novel mechanism. They are transmissible particles that are devoid of nucleic acid. Due to their singular characteristics, Prions emerge as potential danger since they can be used in the development of such weapons. Prions cause fatal infectious diseases, and to date there is no therapeutic or prophylactic approach against these diseases. Furthermore, Prions are resistant to food-preparation treatments such as high heat and can find their way from the digestive system into the nervous system; recombinant Prions are infectious either bound to soil particles or in aerosols. Therefore, lethal Prions can be developed by malicious researchers who could use it to attack political enemies since such weapons cause diseases that could be above suspicion.

  1. Biochemical composition of marine monogenean parasite eggs.

    Science.gov (United States)

    Brazenor, Alexander K; Francis, David S; Hutson, Kate S; Carton, Alexander G

    2017-12-01

    This study on the eggs of the tropical monogenean Neobenedenia girellae presents the first detailed quantitative biochemical information of a marine parasite species' eggs. Moisture and protein composed the majority of the contents of freshly laid eggs (79.12±0.82 and 11.51±0.49% respectively) followed by lipid (2.50±0.15%). Lipids were composed of approximately equal amounts of saturated, monounsaturated, and polyunsaturated fatty acids and the predominant lipid class was triacylglycerol (33.82±1.20%). This study represents a fundamental step towards a better understanding of the early life biology of this important species of parasite. Copyright © 2017. Published by Elsevier B.V.

  2. Biochemical Basis of Sestrin Physiological Activities

    Energy Technology Data Exchange (ETDEWEB)

    Ho, Allison; Cho, Chun-Seok; Namkoong, Sim; Cho, Uhn-Soo; Lee, Jun Hee (Michigan)

    2016-05-10

    Excessive accumulation of reactive oxygen species (ROS) and chronic activation of mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) are well-characterized promoters of aging and age-associated degenerative pathologies. Sestrins, a family of highly conserved stress-inducible proteins, are important negative regulators of both ROS and mTORC1 signaling pathways; however, the mechanistic basis of how Sestrins suppress these pathways remains elusive. In the past couple of years, breakthrough discoveries about Sestrin signaling and its molecular nature have markedly increased our biochemical understanding of Sestrin function. These discoveries have also uncovered new potential therapeutic strategies that may eventually enable us to attenuate aging and age-associated diseases.

  3. Highly valuable microalgae: biochemical and topological aspects.

    Science.gov (United States)

    Pignolet, Olivier; Jubeau, Sébastien; Vaca-Garcia, Carlos; Michaud, Philippe

    2013-08-01

    The past decade has seen a surge in the interest in microalgae culture for biodiesel production and other applications as renewable biofuels as an alternative to petroleum transport fuels. The development of new technologies for the culture of these photosynthetic microorganisms and improved knowledge of their biochemical composition has spurred innovation in the field of high-value biomolecules. These developments are only economically viable if all the microalgae fractions are valorized in a biorefinery strategy. Achieving this objective requires an understanding of microalgae content and the cellular localization of the main biomolecular families in order to develop efficient harvest and sequential recovery technologies. This review summarizes the state of the art in microalgae compositions and topologies using some examples of the main industrially farmed microalgae.

  4. Physico-Biochemical aspects of Shock

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    Kulkarni M.D.

    2009-08-01

    Full Text Available Shock is a clinical condition characterized by decreased blood flow to vital organs due to imbalance between size of vascular bed and effective circulating blood volume and the inability of body tissues to metabolize nutrients normally. The decreased blood flow to vital organs like kidney, liver, spleen, brain etc. is caused by pulling and stagnation blood elsewhere in circulation. The defective blood flow to tissues implies incomplete oxygen supply to the cells, tissues and organs consequent effect being interference with metabolism. It is very essential to study physicobiochemical aspect of shock because after knowing the pathways involved in various physio -pathological processes, we can undertake effective treatment and there by shock can be satisfactorily treated and prevented. Following are the various physico-biochemical processes stated by various workers to study the shock. [Vet. World 2009; 2(4.000: 161-162

  5. Hemoglobin Variants: Biochemical Properties and Clinical Correlates

    Science.gov (United States)

    Thom, Christopher S.; Dickson, Claire F.; Gell, David A.; Weiss, Mitchell J.

    2013-01-01

    Diseases affecting hemoglobin synthesis and function are extremely common worldwide. More than 1000 naturally occurring human hemoglobin variants with single amino acid substitutions throughout the molecule have been discovered, mainly through their clinical and/or laboratory manifestations. These variants alter hemoglobin structure and biochemical properties with physiological effects ranging from insignificant to severe. Studies of these mutations in patients and in the laboratory have produced a wealth of information on hemoglobin biochemistry and biology with significant implications for hematology practice. More generally, landmark studies of hemoglobin performed over the past 60 years have established important paradigms for the disciplines of structural biology, genetics, biochemistry, and medicine. Here we review the major classes of hemoglobin variants, emphasizing general concepts and illustrative examples. PMID:23388674

  6. Dynamic analysis of biochemical network using complex network method

    Directory of Open Access Journals (Sweden)

    Wang Shuqiang

    2015-01-01

    Full Text Available In this study, the stochastic biochemical reaction model is proposed based on the law of mass action and complex network theory. The dynamics of biochemical reaction system is presented as a set of non-linear differential equations and analyzed at the molecular-scale. Given the initial state and the evolution rules of the biochemical reaction system, the system can achieve homeostasis. Compared with random graph, the biochemical reaction network has larger information capacity and is more efficient in information transmission. This is consistent with theory of evolution.

  7. Pattern Selection by Dynamical Biochemical Signals

    Science.gov (United States)

    Palau-Ortin, David; Formosa-Jordan, Pau; Sancho, José M.; Ibañes, Marta

    2015-01-01

    The development of multicellular organisms involves cells to decide their fate upon the action of biochemical signals. This decision is often spatiotemporally coordinated such that a spatial pattern arises. The dynamics that drive pattern formation usually involve genetic nonlinear interactions and positive feedback loops. These complex dynamics may enable multiple stable patterns for the same conditions. Under these circumstances, pattern formation in a developing tissue involves a selection process: why is a certain pattern formed and not another stable one? Herein we computationally address this issue in the context of the Notch signaling pathway. We characterize a dynamical mechanism for developmental selection of a specific pattern through spatiotemporal changes of the control parameters of the dynamics, in contrast to commonly studied situations in which initial conditions and noise determine which pattern is selected among multiple stable ones. This mechanism can be understood as a path along the parameter space driven by a sequence of biochemical signals. We characterize the selection process for three different scenarios of this dynamical mechanism that can take place during development: the signal either 1) acts in all the cells at the same time, 2) acts only within a cluster of cells, or 3) propagates along the tissue. We found that key elements for pattern selection are the destabilization of the initial pattern, the subsequent exploration of other patterns determined by the spatiotemporal symmetry of the parameter changes, and the speeds of the path compared to the timescales of the pattern formation process itself. Each scenario enables the selection of different types of patterns and creates these elements in distinct ways, resulting in different features. Our approach extends the concept of selection involved in cellular decision-making, usually applied to cell-autonomous decisions, to systems that collectively make decisions through cell

  8. Biochemically enhanced methane production from coal

    Science.gov (United States)

    Opara, Aleksandra

    For many years, biogas was connected mostly with the organic matter decomposition in shallow sediments (e.g., wetlands, landfill gas, etc.). Recently, it has been realized that biogenic methane production is ongoing in many hydrocarbon reservoirs. This research examined microbial methane and carbon dioxide generation from coal. As original contributions methane production from various coal materials was examined in classical and electro-biochemical bench-scale reactors using unique, developed facultative microbial consortia that generate methane under anaerobic conditions. Facultative methanogenic populations are important as all known methanogens are strict anaerobes and their application outside laboratory would be problematic. Additional testing examined the influence of environmental conditions, such as pH, salinity, and nutrient amendments on methane and carbon dioxide generation. In 44-day ex-situ bench-scale batch bioreactor tests, up to 300,000 and 250,000 ppm methane was generated from bituminous coal and bituminous coal waste respectively, a significant improvement over 20-40 ppm methane generated from control samples. Chemical degradation of complex hydrocarbons using environmentally benign reagents, prior to microbial biodegradation and methanogenesis, resulted in dissolution of up to 5% bituminous coal and bituminous coal waste and up to 25% lignite in samples tested. Research results confirm that coal waste may be a significant underutilized resource that could be converted to useful fuel. Rapid acidification of lignite samples resulted in low pH (below 4.0), regardless of chemical pretreatment applied, and did not generate significant methane amounts. These results confirmed the importance of monitoring and adjusting in situ and ex situ environmental conditions during methane production. A patented Electro-Biochemical Reactor technology was used to supply electrons and electron acceptor environments, but appeared to influence methane generation in a

  9. Robust simplifications of multiscale biochemical networks

    Directory of Open Access Journals (Sweden)

    Zinovyev Andrei

    2008-10-01

    Full Text Available Abstract Background Cellular processes such as metabolism, decision making in development and differentiation, signalling, etc., can be modeled as large networks of biochemical reactions. In order to understand the functioning of these systems, there is a strong need for general model reduction techniques allowing to simplify models without loosing their main properties. In systems biology we also need to compare models or to couple them as parts of larger models. In these situations reduction to a common level of complexity is needed. Results We propose a systematic treatment of model reduction of multiscale biochemical networks. First, we consider linear kinetic models, which appear as "pseudo-monomolecular" subsystems of multiscale nonlinear reaction networks. For such linear models, we propose a reduction algorithm which is based on a generalized theory of the limiting step that we have developed in 1. Second, for non-linear systems we develop an algorithm based on dominant solutions of quasi-stationarity equations. For oscillating systems, quasi-stationarity and averaging are combined to eliminate time scales much faster and much slower than the period of the oscillations. In all cases, we obtain robust simplifications and also identify the critical parameters of the model. The methods are demonstrated for simple examples and for a more complex model of NF-κB pathway. Conclusion Our approach allows critical parameter identification and produces hierarchies of models. Hierarchical modeling is important in "middle-out" approaches when there is need to zoom in and out several levels of complexity. Critical parameter identification is an important issue in systems biology with potential applications to biological control and therapeutics. Our approach also deals naturally with the presence of multiple time scales, which is a general property of systems biology models.

  10. Moléculas de adhesión: su papel en la fisiopatologIa cardiovascular Adhesion molecules: Their role in cardiovascular physiopathology

    Directory of Open Access Journals (Sweden)

    Ariel Jaitovich

    2004-10-01

    Full Text Available Las moléculas de adhesión son receptores de membrana que participan en diversas funciones vinculadas al tráfico celular, a las interacciones célula-célula y célula-matriz extracelular. Tres grupos de estas moléculas, conocidas como"adhesinas", están relacionados con la enfermedad cardiovascular: integrinas, selectinas y superfamilia de inmunoglobulinas. Intervienen en los fenómenos de activación y disfunción endotelial y se vinculan a la patogenia de la enfermedad coronaria, la injuria por reperfusión, el rechazo del corazón transplantado, la miocarditis, la miocardiopatía hipertrófica, etc. Se asocian también con el mecanismo de acción de las estatinas. El dosaje de los niveles séricos de las moléculas de adhesión tiene valor diagnóstico y predictivo de diversas enfermedades cardiovasculares. Esta revisión enfoca las variadas funciones de las adhesinas y se orienta sobre diversas posibilidades terapéuticas derivadas de su conocimiento.Adhesion molecules are membrane receptors that mediate several functions related to cell traffic, cell-cell interactions, and cell-matrix contact. There are three important groups associated to cardiovascular disease: integrins, selectins, and the immunoglobulin superfamily. They are involved in the endothelial disfunction and activation processes, and are related to the pathogenesis of coronary artery disease, reperfusion injury, allograft vasculopathy, myocarditis, hypertrophic myocardiopathy, etc. Also, they are related to the mechanism of action of statins. Serologic titer of these molecules has diagnostic and predictive value on diverse cardiovascular diseases. This review focuses on the functions of adhesins and discusses various therapeutic possibilities based on their recognition.

  11. Development of 11-Plex MOL-PCR Assay for the Rapid Screening of Samples for Shiga Toxin-Producing Escherichia coli

    Directory of Open Access Journals (Sweden)

    Travis A Woods

    2016-08-01

    Full Text Available Strains of Shiga toxin-producing Escherichia coli (STEC are a serious threat to the public health, with approximately half of the STEC related food-borne illnesses attributable to contaminated beef. We developed an assay that was able to screen samples for several important STEC associated serogroups (O26, O45, O103, O104, O111, O121, O145, O157 and three major virulence factors (eae, stx1, stx2 in a rapid and multiplexed format using the Multiplex oligonucleotide ligation-PCR (MOL-PCR assay chemistry. This assay detected unique STEC DNA signatures and was meant to be used on samples from various sources related to beef production, providing a multiplex and high-throughput complement to the multiplex PCR assays currently in use. Multiplex oligonucleotide ligation-PCR (MOL-PCR is a nucleic acid-based assay chemistry that relies on flow cytometry/image cytometry and multiplex microsphere arrays for the detection of nucleic acid-based signatures present in target agents. The STEC MOL-PCR assay provided greater than 90% analytical specificity across all sequence markers designed when tested against panels of DNA samples that represent different STEC serogroups and toxin gene profiles. This paper describes the development of the 11-plex assay and the results of its validation. This highly multiplexed, but more importantly dynamic and adaptable screening assay allows inclusion of additional signatures as they are identified in relation to public health. As the impact of STEC associated illness on public health is explored additional information on classification will be needed on single samples; thus, this assay can serve as the backbone for a complex screening system.

  12. Development of 11-Plex MOL-PCR Assay for the Rapid Screening of Samples for Shiga Toxin-Producing Escherichia coli.

    Science.gov (United States)

    Woods, Travis A; Mendez, Heather M; Ortega, Sandy; Shi, Xiaorong; Marx, David; Bai, Jianfa; Moxley, Rodney A; Nagaraja, T G; Graves, Steven W; Deshpande, Alina

    2016-01-01

    Strains of Shiga toxin-producing Escherichia coli (STEC) are a serious threat to the health, with approximately half of the STEC related food-borne illnesses attributable to contaminated beef. We developed an assay that was able to screen samples for several important STEC associated serogroups (O26, O45, O103, O104, O111, O121, O145, O157) and three major virulence factors (eae, stx 1 , stx 2) in a rapid and multiplexed format using the Multiplex oligonucleotide ligation-PCR (MOL-PCR) assay chemistry. This assay detected unique STEC DNA signatures and is meant to be used on samples from various sources related to beef production, providing a multiplex and high-throughput complement to the multiplex PCR assays currently in use. Multiplex oligonucleotide ligation-PCR (MOL-PCR) is a nucleic acid-based assay chemistry that relies on flow cytometry/image cytometry and multiplex microsphere arrays for the detection of nucleic acid-based signatures present in target agents. The STEC MOL-PCR assay provided greater than 90% analytical specificity across all sequence markers designed when tested against panels of DNA samples that represent different STEC serogroups and toxin gene profiles. This paper describes the development of the 11-plex assay and the results of its validation. This highly multiplexed, but more importantly dynamic and adaptable screening assay allows inclusion of additional signatures as they are identified in relation to public health. As the impact of STEC associated illness on public health is explored additional information on classification will be needed on single samples; thus, this assay can serve as the backbone for a complex screening system.

  13. Growth kinetics of sintered NiO/ZrO[sub 2](5mol. %Y[sub 2]O[sub 3]) composites

    Energy Technology Data Exchange (ETDEWEB)

    Chen Shueiyuan; Shen Pouyan; Gan Dershin (Inst. of Materials Science and Engineering, National Sun Yat-Sen Univ., Kaohsiung (Taiwan))

    1992-11-01

    This work uses X-ray diffraction and scanning electron microscopy to investigate the solution of NiO and ZrO[sub 2] (with 5 mol% Y[sub 2]O[sub 3], mainly in a metastable t' phase as plasma sprayed powders) and the resultant growth kinetics of the NiO/ZrO[sub 2] composite at 1600degC. The t'-ZrO[sub 2] transforms into cubic (c-)ZrO[sub 2] within 1 h upon firing, and room temperature X-ray lattice parameter results indicate that the solubility of NiO in c-ZrO[sub 2] is about 3 mol%. Within the solubility limit, both sintering and growth of c-ZrO[sub 2] grains were enhanced by NiO addition. A further increase in the NiO content results in the formation of NiO particles which hinder the grain growth of c-ZrO[sub 2]. For specimens with 10 mol% NiO the c-ZrO[sub 2] grains were found to follow the t[sup 1/3] growth kinetics, i.e. bulk diffusion controlled, while the intergranular and intragranular NiO particles coarsen according to t[sup 1/4] and t[sup 1/5] laws respectively. The interfacial energy of NiO/c-ZrO[sub 2] is about 0.48[+-]0.1 J m[sup 2], inferred from the geometry of the NiO particles at the grain boundary. (orig.).

  14. The economic environment of wholesale power generation fuel products at MOL Co, Hungary, and the principal objectives of the trade policy

    International Nuclear Information System (INIS)

    Molnar, I.

    1998-01-01

    The trading conditions of petroleum fuel products are influenced mainly by the liberalized nature of the market. The economic regulations are concentrated to new basic rules: transparent, competition-neutral market aspects, maintaining competition, elimination of market dominance, consumer protection for those without bargaining power, environmental protection for the interest of the society. The fuel market structure and the competition in the region and in Hungary is discussed. The trade policy objectives and tools for MOL are presented, and the trends and prospects for power fuel products and quality development are outlined. (R.P.)

  15. Moléstia de Creutzfeldt-Jakob: estudo clínico, histopatológico e eletromicroscópico de um caso

    Directory of Open Access Journals (Sweden)

    João Guiduoli Neto

    1977-09-01

    Full Text Available É apresentado o caso de paciente de 53 anos de idade com quadro de rebaixamento mental e comprometimento piramidal, com evolução progressiva até mutismo acinético em 6 meses de evolução. Um pneumencefalograma mostrou aumento simétrico do sistema ventricular. Uma biópsia cerebral permitiu encontrar alterações neuronais, gliose e estado espongioso que permitiram classificar o caso como de moléstia de Creutzfeldt-Jakob. São discutidos os achados eletromicroscópicos.

  16. Hirshfeld analysis and mol-ecular docking with the RDR enzyme of 2-(5-chloro-2-oxoindolin-3-yl-idene)-N-methyl-hydrazinecarbo-thio-amide.

    Science.gov (United States)

    Velasques, Jecika Maciel; Gervini, Vanessa Carratu; Kickofel, Lisliane; de Farias, Renan Lira; de Oliveira, Adriano Bof

    2017-05-01

    The acetic acid-catalyzed reaction between 5-chloro-isatin and 4-methyl-thio-semicarbazide yields the title compound, C 10 H 9 ClN 4 OS (I) (common name: 5-chloro-isatin-4-methyl-thio-semicarbazone). The mol-ecule is nearly planar (r.m.s. deviation = 0.047 Å for all non-H atoms), with a maximum deviation of 0.089 (1) Å for the O atom. An S (6) ring motif formed by an intra-molecular N-H⋯O hydrogen bond is observed. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds, forming chains propagating along the a -axis direction. The chains are linked by N-H⋯S hydrogen bonds, forming a three-dimensional supra-molecular structure. The three-dimensional framework is reinforced by C-H⋯π inter-actions. The absolute structure of the mol-ecule in the crystal was determined by resonant scattering [Flack parameter = 0.006 (9)]. The crystal structure of the same compound, measured at 100 K, has been reported on previously [Qasem Ali et al. (2012 ▸). Acta Cryst. E 68 , o964-o965]. The Hirshfeld surface analysis indicates that the most important contributions for the crystal packing are the H⋯H (23.1%), H⋯C (18.4%), H⋯Cl (13.7%), H⋯S (12.0%) and H⋯O (11.3%) inter-actions. A mol-ecular docking evaluation of the title compound with the ribonucleoside diphosphate reductase (RDR) enzyme was carried out. The title compound (I) and the active site of the selected enzyme show Cl⋯H-C (LYS140) , Cg (aromatic ring)⋯H-C (SER71) , H⋯O-C (GLU200) and Fe III ⋯O⋯Fe III inter-molecular inter-actions, which suggests a solid theoretical structure-activity relationship.

  17. Segurança clínica e atividade endoparasiticida de uma nova molécula (Aurixazol) em bovinos e ovinos

    OpenAIRE

    Sakamoto, Cláudio Alessandro Massamitsu [UNESP

    2011-01-01

    O Aurixazol é uma molécula inédita, denominada quimicamente de disofenolato de levamisol. Esta nova formulação, administrada via oral, foi avaliada em quatro estudos: segurança clínica em bovinos (EXPERIMENTO I), eficácias anti-helmínticas em bovinos (EXPERIMENTO II) e ovinos (EXPERIMENTO III) naturalmente infectados, e eficácia residual contra Haemonchus contortus em ovinos experimentalmente infectados (EXPERIMENTO IV). No estudo I, 20 bovinos adultos foram distribuídos em dois grupos iguais...

  18. Morphological, physiological and biochemical responses of camellia oleifera to low-temperature stress

    International Nuclear Information System (INIS)

    Hu, J.; Shu, Q.; Fu, S.; Wu, W.

    2016-01-01

    Camellia oleifera Abel originates from China and is high healthy effect food oil species. It is also a high additional plant in southern China and can help to keep some people of mountain area out of poverty. In recent years, climate change has been abnormal frequently. Abnormal low temperature in winter and late spring coldness may cause the hard hit to C. oleifera farmers. Freezing injury can be caused by sudden decreases in temperature in winter. However, C. oleifera varieties differ in their hardiness to low temperatures. The paper investigated cold-resistance mechanisms by determining and analyzing the morphological, physiological and biochemical characteristics of C. oleifera from eastern, western and southern Anhui, respectively. Sensitivity to low temperature was assessed via the number of leaves in spring shoots, leaf thickness, the activities of protective enzymes such as CAT, POD and SOD, and the inclusion contents of WSS, FPro, MDA, benzene-alcohol extracts and lignin. The results showed that C. oleifera varieties had different physiological and biochemical, and morphological responses to low winter temperatures. In different regions, the number of leaves, leaf thickness, WSS content, FPro content and MDA content varied from 5.2-7.8, 398.79 micro m-465.27 micro m, 23.41 mg/g-24.74 mg/g, 41.86 micro g/g-44.18 micro g/g and 10.08 micro mol/g-14.51 micro mol/g, respectively. The varieties from eastern Anhui, the leaf thickness were thicker. Meanwhile, the protective enzyme activities and inclusion contents were relatively higher. The protective enzyme activities and chemical components contents such as benzene-alcohol extract and lignin represented significantly difference (p<0.05) among three regions. In the future, for the abnormal low temperature in winter, a serious of cultivation measures such as improving the contents of WSS, FPro, benzene-alcohol extract and lignin, were taken to enhance the cold resistance of C. oleifera. The result broadens the

  19. Interpretación biológica acerca de la domesticación del pato criollo (Cairina Moschata

    Directory of Open Access Journals (Sweden)

    1998-01-01

    Full Text Available INTERPRETATION BIOLOGIQUE DE LA DOMESTICATION DU CANARD MUSQUE (CAIRINA MOSCHATA. Cette recherche concerne le processus de domestication de la Cairina Moschata dans les cultures précolombiennes. Elle établit des corrélations entre des données ethno-culturelles, biologiques, des sources historiques écrites. Elle met l'accent sur l'importance de Cairina moschata dans le contrôle exercé sur les insectes, ce qui expliquerait son incorporation à la sphère domestique humaine. L'aire d'origine de ce processus serait celle de la forêt tropicale et sub-tropicale de l'Amérique du Sud, dans la région du Chaco, actuellement partagée entre la Bolivie, le Paraguay et l’Argentine. Esta investigación desarrolla y amplía elementos de discusión acerca del proceso de domesticación de Cairina moschata en las culturas precolombinas. Se correlacionan referencias etnoculturales, biológicas y fuentes escritas de la historia para elaborar y orientar la interpretación. Pone énfasis en la utilidad de Cairina moschata como controlador de insectos y principal motivo para su incorporación a la esfera doméstica del ser humano. Considera los insectos como una presión o determinante ambiental que recibe una respuesta cultural específica con el uso de Cairina moschata. Propone ubicar este proceso en la secuencia cultural de los bosques tropicales y subtropicales de Sudamérica, en la región del Chaco, actualmente compartida por Bolivia, Paraguay y Argentina. A BIOLOGICAL INTERPRETATION ABOUT THE DOMESTICATION OF MUSCOVY DUCK (CAIRINA MOSCHATA. This research discusses the domestication process of the muscovy duck (Cairina moschata within the context of Pre-Columbian cultures. This study contributes new ethno-cultural and biological information and interprets it on the basis of historical sources. This research highlights the use of Cairina moschata to control insects and suggests as the main reason for its inclusion in the human domestic sphere. Insects

  20. El juego de aprender y enseñar el concepto estructurante Evolución Biológica

    Directory of Open Access Journals (Sweden)

    Luis C. Ramírez-Olaya

    2017-01-01

    Full Text Available Se implementó una estrategia didáctica sobre evolución biológica, con tres juegos: Evolución, El cuchador y los fríjoles y Evolucionar o perecer. Esta buscó superar algunos factores que dificultan su comprensión, como lo son la iconografía inapropiada, la insuficiente preparación de los profesores, estrategias didácticas inadecuadas, percepción negativa de la teoría evolutiva y obstáculos presentes en los estudiantes que varían según la edad y que podrán ser conceptuales, lógicos o emocionales. La población corresponde a alumnos de grado noveno y la muestra son 14 participantes de 14 a 18 años de una institución educativa privada de Bogotá D.C. que atiende población con diversidad funcional. La investigación es cualitativa y el enfoque es el análisis de contenido cerrado. Para estimar la eficacia de los juegos, antes y después de cada uno, se plantearon cuestionarios. Los resultados evidencian la efectividad de la estrategia por el aumento de respuestas acertadas, pero persisten obstáculos al aprendizaje. Los más conocidos son: la concepción de los cambios por la necesidad de superar una dificultad que presenta el ambiente o que son dirigidos por un agente externo, el cual puede ser mutagénico y selectivo, produciendo el cambio y seleccionando favorablemente. Además, no considerar que los cambios genéticos puedan ser heredables por la descendencia y que la selección solo actúe en la generación en la que surge el cambio. Asimismo, pensar la evolución como una escala de progreso que lleva a la perfección, es decir, la ortogénesis. El juego media en la construcción de conceptos y ejercita facultades emocionales, focalizando la atención.

  1. Valoración de la conservación biológica en Tunja, Boyacá

    Directory of Open Access Journals (Sweden)

    Aracely Burgos

    2010-10-01

    Full Text Available La biodiversidad brinda servicios ecológicos, económicos y sociales, entre otros, aún así el común de la población ignora sus bondades y beneficios, aunque ella misma es la principal causa de su actual grado de amenaza y extinción. Boyacá es uno de los departamentos con mayor biodiversidad en Colombia y la conservación de las especies presentes en su territorio requiere de conocimiento y esfuerzos conjuntos por parte de sus habitantes. El presente estudio evalúa: la percepción de la ciudadanía frente a la importancia, el compromiso económico, las prioridades, las opciones y el grado de conocimiento, de 7 especies animales y vegetales presentes en la región, nativas y exóticas. Se realizaron 130 encuestas en diferentes zonas de la ciudad. Se encontró que la población tunjana: 1- le da gran importancia a todas las especies de gran tamaño, aunque no sean nativas, 2- estaría dispuesta a pagar por conservar las especies que considera muy importantes; 3- apoyaría el desarrollo de proyectos de producción agropecuaria y generación hidroeléctrica, aun cuando esto signifique la extinción de las especies; 4- opina que las mejores opciones de protección son: aportes internacionales y fondos provenientes del estado y, 5- desconoce las causas y los problemas de conservación de variedades nativas. Hacer efectiva la protección de la biodiversidad en Boyacá requiere, por una parte, un mayor conocimiento biológico y por otra, elaborar políticas y estrategias que tomen en cuenta la opinión del público, por ser ellos quienes realizan la actividad de “conservar”.

  2. Actividad biológica de hongos entomopatógenos sobre Premnotrypes vorax Hustache (Coleoptera: Curculionidae

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Villamil

    2016-06-01

    Full Text Available El gusano blanco (Premnotrypes vorax Hustache ocasiona pérdidas considerables en el cultivo de la papa, las cuales pueden llegar hasta el 100% dependiendo del nivel de infestación y manejo del cultivo. El objetivo del presente estudio fue determinar el efecto individual y combinado de dos aislamientos autóctonos de Beauveria spp. en comparación con dos bioplaguicidas a base de Metarhizium anisopliae y Beauveria brongniartii, sobre P. vorax en condiciones de campo. Se empleó el Diseño Completamente al Azar, con ocho tratamientos y cuatro repeticiones Se hicieron inoculaciónes mediante aspersión dirigida a la base de la planta, utilizando una concentración 5x108 conidias.g-1. Se evaluó porcentaje de daño, porcentaje de control y rendimiento al momento de cosecha (t ha-1. Los resultados indicaron que las cepas comerciales en combinación Metaril® W.P + B. brongniartii® W.P (T6 y el aislamiento autóctono de Beauveria sp. Bv01 (T1, presentaron los menores porcentajes de daño (3,1±0,06 y 3,5±0,2%, los mayores porcentajes de control (77±0,46 y 76,7±1,78%, y la mejor producción (19±0,40t ha-1 y 18±0,25t ha-1 con diferencias significativas (Duncan p≤0,05 respecto a los demás tratamientos y el control regional. Se destacó el T6, ya que mostró el mejor potencial biológico por su rendimiento en cosecha, representando una alternativa promisoria para el control de P. vorax al ser incorporado dentro de un esquema de manejo integrado de la plaga en la región.

  3. Evaluación biológica de la harina de hojas de Macrolobium bicuspidum en pollos de engorde

    Directory of Open Access Journals (Sweden)

    B. Soledad Rodríguez

    2015-09-01

    Full Text Available Los costos de alimentación en la producción de pollos de engorde han llevado a la búsqueda de fuentes alternas de proteínas que permitan sustituir en parte al alimento comercial con follajes locales. El “grifo negro” (Macrolobium bicuspidum es una leguminosa que crece en la zona costera de Venezuela y sus hojas son muy apetecidas por los pollos de los campesinos del pueblo de Yare en Los Valles del Tuy, y en el momento del desmonte de los terrenos circundantes al pueblo, se recoge una gran cantidad de follaje. Se determinó la composición proximal de la hoja de Macrolobium bicuspidum, los contenidos de calcio, magnesio, sodio y potasio, y realizó un ensayo biológico utilizando pollos de engorde que consumieron una dieta control, una dieta aproteica y dietas con inclusión de 5, 10 y 15 % de harina de hojas de M. bicuspidum (HHMb. Se evaluó: aumento de peso, alimento consumido, relación de eficiencia de la proteína (PER, la PER ajustada, utilización neta de las proteínas (NPU, eficiencia alimentaria y conversión alimentaria. Se encontró que la HHMb tiene un porcentaje de proteínas en base seca de 18,6 % y alto contenido de calcio, potasio y magnesio, lo que hace a las hojas atractivas desde el punto de vista nutricional. Al comparar los valores del aumento de peso del grupo de pollos alimentados con la dieta control con los alimentados con las dietas en las cuales se sustituyó la proteína control con 5, 10 y 15 % de HHMb, no se encontraron diferencias significativas (p > 0,05 entre el grupo control y el grupo con sustitución de proteína con un 5 % de HHMb, pero sí hubo diferencias significativas (p < 0,05 con los otros grupos. La dieta con 5 % de HHMb fue la mejor dieta luego de la dieta control, indicando esto que puede incorporarse en pequeñas cantidades en alimentos para aves.

  4. Sintering behavior of ultra-fine Al2O3-(ZrO2 + X mol% Y2O3) ceramics by high-frequency induction heating

    International Nuclear Information System (INIS)

    Kim, S.W.; Cockcroft, S.L.; Khalil, K.A.; Ogi, K.

    2010-01-01

    Alumina-zirconia composites consisting of Al 2 O 3 -20 vol% yttria-stabilized zirconia containing two types of yttria-stabilized zirconia, 3 mol% yttria doped ZrO 2 (3YSZ) and 8 mol% yttria doped ZrO 2 (8YSZ), were consolidated very rapidly to full density by high-frequency induction heating and sintering (HFIHS). A comparison was made between the sinter behavior and the mechanical properties (hardness, fracture toughness and flexural strength) of the 3YSZ and 8YSZ material. In addition, the consolidated samples were analyzed for phase content by X-ray diffraction (XRD) and the fracture surfaces were examined using a scanning electron microscopy (SEM). The results showed that 3YSZ is most effective as a second phase for the toughening of alumina in comparison to the 8YSZ material. Al 2 O 3 -3YSZ composites exhibiting relatively good mechanical properties and limited grain growth were successfully synthesized at relatively low temperatures using this technique.

  5. Synthesis and crystallization behavior of 3 mol% yttria stabilized tetragonal zirconia polycrystals (3Y-TZP) nanosized powders prepared using a simple co-precipitation process

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, Yu-Wei [Graduate Institute of Applied Science, National Kaohsiung University of Applied Sciences, 415 Chien-Kung Road, Kaohsiung 80782, Taiwan (China); Yang, Ko-Ho, E-mail: yangkoho@cc.kuas.edu.tw [Graduate Institute of Applied Science, National Kaohsiung University of Applied Sciences, 415 Chien-Kung Road, Kaohsiung 80782, Taiwan (China); Department of Mold and Die Engineering, National Kaohsiung University of Applied Sciences, 415 Chien-Kung Road, Kaohsiung 80782, Taiwan (China); Chang, Kuo-Ming [Department of Mechanical Engineering, National Kaohsiung University of Applied Sciences, 415 Chien-Kung Road, Kaohsiung 80782, Taiwan (China); Dental Materials Research Center, National Kaohsiung University of Applied Sciences, 415 Chien-Kung Road, Kaohsiung 80782, Taiwan (China); Yeh, Sung-Wei [Metal Industries Research and Development Centre, 1001 Kaohsiung Highway, Kaohsiung 811, Taiwan (China); Wang, Moo-Chin, E-mail: mcwang@kmu.edu.tw [Department of Fragrance and Cosmetics Science, Kaohsiung Medical University, 100, Shihchuan 1st Road, Kaohsiung 80728, Taiwan (China)

    2011-06-16

    Highlights: > The thermal behavior of 3Y-TZP precursor powders had been investigated. > The crystallization behavior of 3Y-TZP nanopowders had been investigated. > The activation energy for crystallization of tetragonal ZrO{sub 2} was obtained. > The growth morphology parameter n is approximated as 2.0. > The crystallites show a plate-like morphology. - Abstract: The synthesis and crystallization behavior of 3 mol% yttria stabilized tetragonal zirconia polycrystals (3Y-TZP) nanopowders prepared using a simple co-precipitation process at 348 K and pH = 7 were investigated using differential scanning calorimetry/thermogravimetry (DSC/TG), an X-ray diffractometer (XRD), the Raman spectra, transmission electron microscopy (TEM), selected area electron diffraction (SAED), and an energy dispersive spectrometer (EDS). The activation energy of tetragonal ZrO{sub 2} crystallization from 3Y-TZP freeze-dried precursor powders using a non-isothermal method, namely, 169.2 {+-} 21.9 kJ mol{sup -1}, was obtained. The growth morphology parameter n was approximated as 2.0, which indicated that it had a plate-like morphology. The XRD, Raman spectra, and SAED patterns showed that the phase of the tetragonal ZrO{sub 2} was maintained at 1273 K. The crystallite size of 3Y-TZP freeze-dried precursor powders calcined at 1273 K for 5 min was 21.3 nm.

  6. Crystallization kinetics and growth mechanism of 8 mol% yttria-stabilized zirconia (8YSZ) nano-powders prepared by a sol-gel process

    Energy Technology Data Exchange (ETDEWEB)

    Kuo, C.-W. [Department of Resources Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Lee, Y.-H. [Department of Materials Science and Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Hung, I-M. [Department of Chemical Engineering and Materials Science, Yuan Ze University, 135 Far-East Road, Chung-Li, Taoyuan, Taiwan (China); Wang, M.-C. [Faculty of Fragrance and Cosmetics, Kaohsiung Medical University, 100 Shi-Chuan 1st Road, Kaohsiung 807, Taiwan (China); Wen, S.-B. [Department of Resources Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Fung, K.-Z. [Department of Materials Science and Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Shih, C.-J. [Faculty of Fragrance and Cosmetics, Kaohsiung Medical University, 100 Shi-Chuan 1st Road, Kaohsiung 807, Taiwan (China)], E-mail: cjshih@kmu.edu.tw

    2008-04-03

    Eight mol% yttria-stabilized zirconia (8YSZ) gel powders were synthesized at 348 K for 2 h using ZrOCl{sub 2}.8H{sub 2}O and Y(NO{sub 3}){sub 3}.6H{sub 2}O as starting materials in an ethanol-water solution by a sol-gel process. The crystallization kinetics and growth mechanism of the 8YSZ gel powders have been investigated using differential thermal analysis (DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). The XRD results and SAED pattern show that the 8YSZ gel powders calcined at 773 K for 2 h is a cubic ZrO{sub 2}. The activation energy for the crystallization of the cubic ZrO{sub 2} formation in the 8YSZ gel powders is determined as 231.76 kJ/mol by a non-isothermal DTA method. Both growth morphology parameter (n) and crystallization mechanism index (m) are close to 3.0, indicating that the bulk nucleation is dominant in the cubic ZrO{sub 2} formation. The TEM examination shows that the cubic ZrO{sub 2} has a spherical-like morphology with a size ranging from 10 to 20 nm.

  7. Crystallization kinetics and growth mechanism of 8 mol% yttria-stabilized zirconia (8YSZ) nano-powders prepared by a sol-gel process

    International Nuclear Information System (INIS)

    Kuo, C.-W.; Lee, Y.-H.; Hung, I-M.; Wang, M.-C.; Wen, S.-B.; Fung, K.-Z.; Shih, C.-J.

    2008-01-01

    Eight mol% yttria-stabilized zirconia (8YSZ) gel powders were synthesized at 348 K for 2 h using ZrOCl 2 .8H 2 O and Y(NO 3 ) 3 .6H 2 O as starting materials in an ethanol-water solution by a sol-gel process. The crystallization kinetics and growth mechanism of the 8YSZ gel powders have been investigated using differential thermal analysis (DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). The XRD results and SAED pattern show that the 8YSZ gel powders calcined at 773 K for 2 h is a cubic ZrO 2 . The activation energy for the crystallization of the cubic ZrO 2 formation in the 8YSZ gel powders is determined as 231.76 kJ/mol by a non-isothermal DTA method. Both growth morphology parameter (n) and crystallization mechanism index (m) are close to 3.0, indicating that the bulk nucleation is dominant in the cubic ZrO 2 formation. The TEM examination shows that the cubic ZrO 2 has a spherical-like morphology with a size ranging from 10 to 20 nm

  8. Synthesis and crystallization behavior of 3 mol% yttria stabilized tetragonal zirconia polycrystals (3Y-TZP) nanosized powders prepared using a simple co-precipitation process

    International Nuclear Information System (INIS)

    Hsu, Yu-Wei; Yang, Ko-Ho; Chang, Kuo-Ming; Yeh, Sung-Wei; Wang, Moo-Chin

    2011-01-01

    Highlights: → The thermal behavior of 3Y-TZP precursor powders had been investigated. → The crystallization behavior of 3Y-TZP nanopowders had been investigated. → The activation energy for crystallization of tetragonal ZrO 2 was obtained. → The growth morphology parameter n is approximated as 2.0. → The crystallites show a plate-like morphology. - Abstract: The synthesis and crystallization behavior of 3 mol% yttria stabilized tetragonal zirconia polycrystals (3Y-TZP) nanopowders prepared using a simple co-precipitation process at 348 K and pH = 7 were investigated using differential scanning calorimetry/thermogravimetry (DSC/TG), an X-ray diffractometer (XRD), the Raman spectra, transmission electron microscopy (TEM), selected area electron diffraction (SAED), and an energy dispersive spectrometer (EDS). The activation energy of tetragonal ZrO 2 crystallization from 3Y-TZP freeze-dried precursor powders using a non-isothermal method, namely, 169.2 ± 21.9 kJ mol -1 , was obtained. The growth morphology parameter n was approximated as 2.0, which indicated that it had a plate-like morphology. The XRD, Raman spectra, and SAED patterns showed that the phase of the tetragonal ZrO 2 was maintained at 1273 K. The crystallite size of 3Y-TZP freeze-dried precursor powders calcined at 1273 K for 5 min was 21.3 nm.

  9. Biochemical diagnosis of pheochromocytoma: which test is best?

    NARCIS (Netherlands)

    Lenders, J.W.M.; Pacak, K.; Walther, M.M.; Linehan, W.M.; Mannelli, M.; Friberg, P.; Keiser, H.R.; Goldstein, D.S.; Eisenhofer, G.

    2002-01-01

    CONTEXT: Diagnosis of pheochromocytoma depends on biochemical evidence of catecholamine production by the tumor. However, the best test to establish the diagnosis has not been determined. OBJECTIVE: To determine the biochemical test or combination of tests that provides the best method for diagnosis

  10. Biochemical changes occurring during fermentation of camel milk by ...

    African Journals Online (AJOL)

    Biochemical changes occurring during fermentation of camel milk by selected bacterial starter cultures. ... Abstract. The biochemical changes in amino acids, water soluble vitamins, soluble sugars and organic acids occurring during fermentation (at 43°C for 6 h) of camel milk inoculated with Streptococcus thermophilus 37, ...

  11. Biochemical markers of mineral bone disorder in South African ...

    African Journals Online (AJOL)

    Associations between log transformed PTH and other biochemical parameters were assessed by multiple linear regression analyses following significant associa- tions obtained from univariate regression analyses. A lo- gistic regression model was used to evaluate the effect of other biochemical parameters on the odds of ...

  12. Developments in commercially produced microbials at Biochem Products

    Science.gov (United States)

    John Lublinkhof; Douglas H. Ross

    1985-01-01

    Biochem Products is part of a large industrial and scientific family - the Solvay Group. Solvay, headquartered in Brussels, Belgium is a multinational company with 46,000 employees worldwide. In the U.S., our working partners include a large polymer manufacturer, a peroxygen producer and a leading poultry and animal health products company. Biochem Products is a...

  13. Exploring basic biochemical constituents in the body tissues of ...

    African Journals Online (AJOL)

    Feeding regime did not influence susceptibility to mass loss during export. Animal age influenced the biochemical composition and export performance of abalone. Keywords: abalone; aquaculture; feeds; Haliotis midae; live export; mass loss; tissue biochemical constituents. African Journal of Marine Science 2010, 32(1): ...

  14. Biochemical evaluation of phenylketonuria (PKU: from diagnosis to treatment

    Directory of Open Access Journals (Sweden)

    Leticia Belmont-Martínez

    2014-07-01

    Besides periodical Phe and Tyr testing, biochemical follow-up includes the measurement of necessary elements that guarantee normal physical and intellectual development such as selenium, zinc, B12 vitamin, folates, iron and long chain fatty acids. Clinical context is as important as biochemical status so periodic evaluation of nutritional, medical, social and psychological aspects should be included.

  15. Reference Ranges for Some Biochemical Parameters in Adult ...

    African Journals Online (AJOL)

    PURPOSE: To establish the reference ranges of some biochemical parameters for adult Kenyan population. METHODS: In a prospective involving 1100 healthy blood donors (age: 18-55 yr) in Kenyatta National Hospital, Kenya reference ranges of some biochemical analytes were constructed by using the parametric ...

  16. An improved solution of first order kinetics for biochemical oxygen ...

    African Journals Online (AJOL)

    This paper evaluated selected Biochemical Oxygen Demand first order kinetics methods. Domesticinstitutional wastewaters were collected twice in a month for three months from the Obafemi Awolowo University, Ile-Ife waste stabilization ponds. Biochemical Oxygen Demand concentrations at different days were determined ...

  17. Biochemical characterization of three Aspergillus niger β-galactosidases

    Directory of Open Access Journals (Sweden)

    Dandan Niu

    2017-05-01

    Conclusions: Three new β-galactosidases belonging to glycosyl hydrolase family 35 from A. niger F0215 were cloned and biochemically characterized. In addition to the known LacA, A. niger has at least three β-galactosidase family members with remarkably different biochemical properties.

  18. Haematological and blood biochemical indices of West African ...

    African Journals Online (AJOL)

    Haematological and blood biochemical indices of West African dwarf goats vaccinated against Pestes des petit ruminants (PPR) ... blood biochemical indices of forty randomly selected West African dwarf (WAD) goats were studied. Packed cell volume ... neutrophil/lymphocyte ratio and white blood cells (WBC) than females.

  19. Differential response of biochemical parameters to EMS and MMS ...

    African Journals Online (AJOL)

    B.B.D. Khalandar

    2015-06-10

    Jun 10, 2015 ... Abstract Aim: To study the effect of alkylating agents such as EMS and MMS on chromosomes and biochemical parameters in induced diabetic mouse. Methods: Chromosome preparations from bone marrow was made using the method of Evans et al. (1964) and biochemical estimations from the liver was ...

  20. Possible Biochemical Markers in Protein-Energy Malnutrition and ...

    African Journals Online (AJOL)

    This study was carried out to determine possible biochemical markers in children suffering from Plasmodium falciparum malaria and Protein-Energy Malnutrition in a Hospital setting in Western Kenya. Spectrophotometric assays of selected biochemical parameters namely, albumin, total proteins, glucose, glutamate ...

  1. Local biochemical and morphological differences in human Achilles tendinopathy

    DEFF Research Database (Denmark)

    Pingel, Jessica; Fredberg, U.; Qvortrup, Klaus

    2012-01-01

    The incidence of Achilles tendinopathy is high and underlying etiology as well as biochemical and morphological pathology associated with the disease is largely unknown. The aim of the present study was to describe biochemical and morphological differences in chronic Achilles tendinopathy....... The expressions of growth factors, inflammatory mediators and tendon morphology were determined in both chronically diseased and healthy tendon parts....

  2. Qualidade Química na Compostagem de Resíduos Urbanos com Inoculação Biológica

    Directory of Open Access Journals (Sweden)

    Egon Nord

    2014-10-01

    Full Text Available A compostagem é um processo eficiente para estabilização dos resíduos orgânicos que pode ser melhorada com o uso de inoculantes biológicos para uso na agricultura orgânica. Objetivou-se avaliar o efeito de um inoculante biológico na qualidade química de composto orgânico produzido com alguns resíduos urbanos. No experimento de compostagem utilizou-se o delineamento inteiramente casualizado em esquema fatorial 2x2, sendo dois resíduos de alta relação C:N (bagaço de cana e folhas de varrição e dois níveis de inoculação (com e sem. Foram avaliados teores de macro e micronutrientes, C, pH e a demanda química de oxigênio nos compostos produzidos. A temperatura e a demanda química de oxigênio (DQO foram os melhores indicadores do processo de compostagem dos materiais testados. Para as condições deste estudo, o inoculante biológico testado não alterou os teores de macro e micronutrientes e não acelerou o processo de compostagem. Conclui-se que há ampla possibilidade de transformação de resíduos orgânicos urbanos em insumos agrícolas, através da aplicação de tecnologia

  3. Riesgos biológico y químico en planta de compostaje de ingenio azucarero, Valle del Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Ivá Darío López Villalobos

    2016-12-01

    Full Text Available El riesgo químico y biológico, son los principales riesgos a los que se exponen los colaboradores de la planta de compostaje del ingenio azucarero, es sin duda una posible causa de enfermedades de tipo respiratorio y de la piel, así como de enfermedades infecciosas, lo cual afecta directamente la productividad de la empresa. Se utilizó un estudio descriptivo cuya metodología se realizó en tres fases, una primera de diagnóstico e identificación de peligros, en seguida según los resultados de las listas de chequeo, se realizó una matriz DOFA, luego los árboles de problemas se convirtieron en arboles de objetivos remplazando cada uno de los problemas y consecuencias por las soluciones, así se definieron los objetivos prioritarios a emprender, para alcanzar las acciones de mejora en el proceso del compostaje. De los resultados obtenidos se resalta que el riesgo biológico tiene una interpretación de riesgo III (alto, mejorable y representa el 55,6%, mientras que el riesgo químico con una interpretación de riesgo I (muy alto, crítico representa el 44,4%. También debe mencionarse que la planta de compostaje presenta un déficit en las medidas de prevención y protección por el incumplimiento de la normatividad legal vigente en seguridad y salud. Es importante que la planta de compostaje empiece a gestionar el cumplimiento de lo establecido en el programa de intervención propuesto para la misma, para de esta forma disminuir la vulnerabilidad del personal expuesto a riesgos biológico y químico.

  4. Cría masiva y uso de ninfas de Rhodnius pallescens, Barber, 1932 (Hemiptera, Reduviidae, Triatominae para ensayos biológicoso

    Directory of Open Access Journals (Sweden)

    Mónica Flórez M

    2015-04-01

    Full Text Available Introducción: Rhodnius pallescens es una especie silvestre que hace intrusión a las viviendas en zonas en las cuales se han presentado brotes de Chagas agudo en Colombia; y para el estudio de sus características biológicas y el monitoreo de la susceptibilidad o resistencia de poblaciones de campo a insecticidas, se requiere del uso de una gran cantidad de insectos mantenidos en el laboratorio. Objetivo: Establecer condiciones de cría de ninfas de R. pallescens que permitan su mejor aprovechamiento en ensayos biológicos. Metodología: Se utilizó una cepa de laboratorio de R. pallescens proveniente de San Martin (Cesar, Colombia y se realizaron bioensayos para: estimar el tiempo de alimentación, establecer condiciones de cría, estimar tiempos de desarrollo ninfal y comparar la fecundidad usando dos fuentes de alimentación y varios tipos de soporte. Resultados: 60 minutos de ofrecimiento de alimento permite la alimentación de 95% de las ninfas. El promedio de oviposición diario/hembra fue de 2,7 huevos y no varío significativamente con el consumo de sangre de gallina o ratón. La duración promedio del ciclo de vida desde huevo hasta el estadio ninfa-V fue de 128,6 días. El uso de cartulina negra y plumas dentro de los frascos de cría mejora la oviposición. Ninfas-V alimentadas desde ninfa-I y pesadas a los 5 o 6 días permite un aprovechamiento del 89% de las ninfas. Conclusiones: Los resultados de este trabajo brindan conocimiento para la cría masiva y el uso de ninfas de Rhodnius pallescens en ensayos biológicos.

  5. Marcadores tumorais no câncer de pulmão: um caminho para a terapia biológica

    OpenAIRE

    PACHECO FERNANDO AZEVEDO; PASCHOAL MARCOS EDUARDO MACHADO; CARVALHO MARIA DA GLÓRIA DA COSTA

    2002-01-01

    Os avanços recentes na genética e na biologia molecular permitiram a identificação de genes e proteínas produzidos ou superexpressados pelos tumores. Tais produtos, os chamados marcadores tumorais, antes utilizados apenas como ferramentas de diagnóstico e prognóstico, vêm atualmente tomando papel importante no desenvolvimento de novas modalidades de tratamento, direcionadas a quebrar o ciclo biológico da progressão tumoral. Neste artigo, revisa-se o papel de alguns marcadores tumorais tradici...

  6. Uso y verificación con indicadores biológicos en esterilizadores de cirujanos dentistas de San Luis Potosí, México

    OpenAIRE

    Patiño-Marín Nuria; Loyola-Rodríguez Juan Pablo; Tovar-Reyes Luis Fernando

    2001-01-01

    Objetivo. Conocer el uso y verificar los ciclos de esterilización con indicadores biológicos en los equipos utilizados por cirujanos dentistas de la Facultad de Estomatología de la Universidad Autónoma de San Luis Potosí (UASLP) y del Colegio Dental Potosino. Material y métodos. Estudio transversal hecho en 1999-2000. El 65% (n=130) de los odontólogos participaron con un esterilizador, la verificación se realizó por indicadores que contenían esporas de Bacillus subtilis y de Bacillus stearoth...

  7. Estudio de un modelo in vivo de los mecanismos de acción implicados en la actividad biológica de polifenoles

    OpenAIRE

    Sánchez Hernández, Eva

    2017-01-01

    Trabajo de fin de grado. Grado en Biología. Curso académico 2016-2017 Los flavonoides en la dieta se han relacionado con propiedades beneficiosas para la salud, lo cual ha sido atribuido en parte a sus propiedades antioxidantes, como se demostró en muchos estudios in vitro. En el organismo humano la mayoría de los flavonoides son poco biodisponibles y en gran medida biotransformados en diferentes metabolitos que podrían contribuir a los efectos biológicos de sus flavonoides originales. ...

  8. Efectos adversos metabólicos y nutricionales asociados a la terapia biológica del cáncer

    OpenAIRE

    Sara Alonso Castellanos; María Soto Célix; Judith Alonso Galarreta; Ana del Riego Valledor; Alberto Miján de la Torre

    2014-01-01

    Introducción: Los diferentes tipos de cáncer constituyen una de las principales causas de morbi-mortalidad en el mundo. La terapia clásica antitumoral (cirugía, quimioterapia, radioterapia) ha incrementado notoriamente la supervivencia. Las terapias biológicas, con mecanismos de acción selectivos y frecuentemente específicos, constituyen una incorporación relativamente reciente al tratamiento oncológico; entre las más utilizadas se incluyen: citoquinas, anticuerpos monoclonales e inhibidores ...

  9. Atividade biológica de células-tronco da polpa de dentes decíduos humanos submetidas à criopreservação

    OpenAIRE

    Antunes, Fernanda Ginani

    2013-01-01

    Células-tronco da polpa dental humana têm sido amplamente investigadas em razão da sua capacidade de diferenciar-se tanto em células dentais quanto não dentais, com potencial de utilização em terapias envolvendo a engenharia de tecidos. A técnica de criopreservação celular representa uma alternativa viável para a conservação dessas células, já que cessa reversivelmente, de forma controlada, todas as suas funções biológicas em uma temperatura ultra-baixa. O pr...

  10. Mejora de los indicadores biológicos de exposición al mercurio en trabajadores de una refinería de oro

    Directory of Open Access Journals (Sweden)

    Augusto V. Ramírez

    2011-07-01

    Full Text Available Introducción: La exposición al mercurio asociada a malas prácticas de higiene laboral favorece el desarrollo de la intoxicación ocupacional denominada mercurialismo. Para verificar el impacto que la mejoría de los niveles ambientales de mercurio tendría en trabajadores de la refinería en una empresa aurífera peruana, se elaboró un programa de reingeniería para el control de la exposición que abarca aspectos de salud e higiene laboral y participación activa de la jefatura operativa. Objetivos: Estudiar en trabajadores de una refinería aurífera las variaciones de indicadores de exposición a mercurio al mejorar la calidad del ambiental laboral. Diseño: Estudio prospectivo y descriptivo. Lugar de estudio: Servicio de Salud Ocupacional de una empresa minera aurífera en el Perú. Participantes: Trabajadores de la refinería de oro. Intervenciones: Se modificó la metodología del examen médico y de la evaluación del ambiente laboral de 100 trabajadores de la refinería de oro, durante el periodo 2003-2007, además de mejorar las condiciones de trabajo. Luego, se cuantificó semestralmente los límites ambientales del tóxico y los indicadores de exposición. Se analizó estadísticamente la variación de los valores medios de los indicadores biológicos respecto a la variación del tóxico en el ambiente de trabajo. Principales medidas de resultados: Mejora de los indicadores ambientales y biológicos. Resultados: Los valores medios de los indicadores ambientales mejoraron cada año, al igual que los indicadores biológicos del universo estudiado. El mercurio ambiental en 2 003 fue 6,3 mg/m3 y, en 2 007, 0,03, valor de p < 0,001. El indicador mercurio urinario en 2003 fue 11,9 ug/L y, en 2007, 2,3 con p < 0,01. Conclusiones: En el grupo estudiado, la disminución del tóxico en el ambiente de labor determinó la mejora significativa de los indicadores biológicos de exposición al mercurio en el trabajador.

  11. Estimación de parámetros en modelos biológicos complejos. Aplicación a modelos de crecimiento tumoral

    OpenAIRE

    Fernández Slezak, Diego

    2010-01-01

    Las ciencias de la vida comprenden todos los campos de la ciencia que estudian organismos vivos y procesos biológicos. Tradicionalmente, la biología y la medicina han sido los campos centrales de las ciencias de la vida. Los avances tecnológicos en biología molecular y biotecnología trajeron consigo un crecimiento sin precedentes de campos interdisciplinarios, tales como la bioinformática, biología computacional, biología de sistemas, y neuroinformática por nombrar algunas. En particular, el ...

  12. Pontos positivos e negativos do estágio supervisionado na perspectiva dos licenciandos em ciências biológicas

    OpenAIRE

    Barreto, Edna Silva

    2013-01-01

    O objetivo deste artigo foi identificar as principais contribuições e dificuldades vivenciadas por licenciandos de ciências biológicas durante o estágio supervisionado. A pesquisa teve um caráter qualitativo, sendo a coleta de dados realizada com licenciandos do nono período, os quais responderam a um questionário com deaz questões abertas. A interpretação das informações foi realizada á luz da análise de conteúdo. Os resultados revelaram que as contribuições mais citadas foram: a elaboração ...

  13. Modelos matemáticos para reactores biológicos de lecho empacado (pbr): una revisión bibliográfica

    OpenAIRE

    Corredor, Deisy; Caicedo, Luis Alfonso

    2010-01-01

    El modelo matemático y análisis teórico de reactores biológicos de lecho empacado (PBR) ha sido estudiado por diferentes autores, quienes tuvieron en cuenta variedad de cinéticas de reacción, modelos unidimensionales, homogéneos, pseudohomogéneos y heterogéneos. Las ecuaciones resultantes del modelo fueron solucionadas, en su gran mayorIa, por sistemas de métodos numéricos. Se ha analizado en estos el efecto de variables de proceso de importancia fIsica con respecto a parámetros de diseno y o...

  14. Brasicáceas y perspectivas de control biológico del insecto plaga Plutella xylostella (Lepidóptera: Plutellidae utilizando Bacillus thuringiensis

    Directory of Open Access Journals (Sweden)

    Julieth Mena Guerrero

    2017-09-01

    Full Text Available La Plutella xylostella (L. es un insecto plaga que afecta principalmente los cultivos de brasicáceas o crucíferas como el repollo, coliflor, brócoli y rábano, entre otros. Las limitaciones del rendimiento y calidad de estos cultivos se deben principalmente al difícil manejo de esta plaga. En la actualidad, para controlar la P. xylostella se utilizan insecticidas de síntesis química, como piretroides, carbamatos u organofosforados, los cuales tienen acción inmediata pero causan efectos adversos en la salud y en el ambiente. Adicionalmente, la ausencia de enemigos naturales capaces de controlar a sus poblaciones, y su resistencia a numerosos insecticidas convencionales hacen que este insecto se establezca rápidamente en áreas productoras de brasicáceas. Se calcula que los costos mundiales asociados al control de la P. xylostella, sumados a las pérdidas en la producción agrícola, están entre cuatro y cinco billones de dólares anuales. Se han propuesto alternativas seguras, efectivas y de menor impacto ambiental, como el control biológico, que permite la producción sostenible de los cultivos de brasicáceas. La industria agrícola y forestal reconoce a la bacteria entomopatógena Bacillus thuringiensis (Bt como una buena alternativa biológica a los insecticidas químicos, puesto que es inocua sobre el ambiente y su toxicidad es altamente selectiva, ligada a su estrecho rango de especificidad sobre diferentes insectos plaga de los órdenes Lepidóptera, Coleóptera y Díptera. En esta revisión se presentan las posibilidades actuales que se pueden emplear para el control de la P. xylostella utilizando modelos investigativos basados en ensayos biológicos con Bt. Dichas posibilidades buscan superar las desventajas existentes en relación con la plaga P. xylostella y los cultivos de brasicáceas, además de entender la fisiología de Bt bajo condiciones que permitan incrementar la eficacia en el control biológico.

  15. Es el reimplante valvular mejor que el bentall biológico modificado para tratar aneurismas de la raíz asociados a insuficiencia?

    OpenAIRE

    Obando López, Carlos Eduardo; Bresciani, Renato; Camacho, Jaime; Carreño Jaimes, Marisol; Gutiérrez, Héctor; Sandoval, Néstor; Umaña Mallarino, Juan Pablo

    2010-01-01

    Es el reimplante valvular mejor que el Bentall Biológico Modificado para tratar aneurismas de la raíz asociados a insuficiencia? Obando CE; Gutiérrez HF; Santamaría G, Bresciani R; Camacho J; Sandoval N; Umaña J. Departamento de Cirugía Cardiovascular, Fundación Cardio Infantil, Bogotá, Colombia. Objetivo: comparar resultados funcionales, morbilidad y sobrevida a corto y mediano plazo, tras la realización de Bentall modificado con prótesis Freestyle vs reimplante valvular de Tirone David, en ...

  16. Comparación visual de grandes jerarquías: El caso de las de taxonomías biológicas

    Directory of Open Access Journals (Sweden)

    Liliana Sancho-Chavarría

    2017-03-01

    Full Text Available La visualización y el análisis de las taxonomías biológicas es un reto monumental: las taxonomías biológicas son voluminosas, pueden cambiar con el tiempo conforme la comunidad científica hace nuevos descubrimientos, la información taxonómica está dispersa en todo el mundo, está incompleta y existen diferentes versiones de las taxonomías ya que los científicos utilizan diferentes criterios de clasificación. Para entender y documentar mejor la biodiversidad, así como para apoyar su conservación, es necesario curar e integrar esta información. Es aquí donde la comparación de grandes jerarquías se convierte en una herramienta sumamente útil. Hasta el momento, la investigación sobre este tema incluye el estado del arte sobre la visualización de la información y técnicas de comparación de jerarquías, el desarrollo de una herramienta de visualización de jerarquías mediante la técnica de árboles de conos y la caracterización de las tareas del trabajo taxonómico que inducen cambios en las taxonomías. Se identificaron 10 tareas: identificar congruencia, identificar correcciones, identificar nuevos nodos, obtener una visión global de los cambios, resumir cambios, encontrar inconsistencias, aplicar filtros, obtener detalles, enfocarse y editar. La contribución de esta investigación es comparar y visualizar las taxonomías biológicas mediante la consideración sistemática de dicha caracterización de las tareas de los usuarios, lo cual es un enfoque novedoso para el desarrollo de propuestas de visualización en el dominio de taxonomías biológicas.

  17. La enseñanza de la Antropología Biológica en la Universidad de la República, Uruguay

    Directory of Open Access Journals (Sweden)

    Sans, Mónica

    2005-01-01

    Full Text Available En 1976 comienza a funcionar la Licenciatura en Ciencias Antropológicas, con un currículo copiad del Universidad de Buenos Aires y especializaciones en Etnología y en Arqueología, en la órbita de la Facultad de Humanidades y Ciencias; incluía un curso de Antropología Física, situación que se mantiene en las modificaciones de Planes de 1987 y 1991. La separación de la Facultad de Humanidades, en 1991, de la de Ciencias dificulta más tanto el acceso a cursos optativos como la investigación en temas afines. En el presente año, apoyado por nuestra Facultad en conjunto con Medicina, Ciencias, y posteriormente Odontología, se crea la Licenciatura en Biología Humana, con un currículo flexible (33% de créditos en materias biológicas, 22% en básicas, 8% en humanísticas, y 37% según el perfil del estudiante. Si bien con algunos problemas de funcionamiento, hay actualmente anotados 100 estudiantes. Es claro que un único curso de Antropología Biológica (96 horas no es más que una introducción a los diversos temas del área. La no existencia de una especialización, como la hay en Arqueología o Antropología Social, lleva a los estudiantes a optar por una de estas, con el consiguiente desánimo; dentro de lo posible cursan optativas del área biológica. Llama la atención la falta de cursos de Antropología Biológica en carreras como Biología o Medicina. Con relación a la Licenciatura en Biología Humana, aunque no se plantean cursos específicos, podría dar solución a la formación de bioantropólogos.

  18. La infección por el virus del papiloma humano, un posible marcador biológico de comportamiento sexual en estudiantes universitarios

    OpenAIRE

    Sánchez-Alemán Miguel A; Uribe-Salas Felipe; Conde-González Carlos J

    2002-01-01

    Objetivo. Estimar la prevalencia de infección por el virus del papiloma humano (VPH) en estudiantes universitarios y utilizar dicha frecuencia como un marcador biológico para evaluar el comportamiento sexual. Material y métodos. Se realizó un estudio transversal, en estudiantes de la Universidad Autónoma del estado de Morelos, México, durante el periodo 2000-2001. Se aplicó un cuestionario y se colectaron muestras genitales para detectar ADN de los VPH oncogénicos. Los datos se analizaron uti...

  19. Estudio de distribución de tiempos de residencia en un reactor biológico de lecho empacado cerámico

    OpenAIRE

    Tatiana Rodríguez Chaparro; Luz Helena Pinzón; Carlos Alberto Arámbula

    2004-01-01

    La distribución de tiempos de residencia de un reactor es una característica del mezclado que ocurre dentro de él [ ] 1 [ ] 2 ; su determinación es básica para el diseño de cualquier tipo de reactor en escala real. El objetivo de esta investigación consistió en determinar la distribución de tiempos de residencia en un reactor biológico de lecho empacado cerámico (anillos) a partir de pruebas con trazadores. Los resultados obtenidos utilizando las técnicas de inyección por paso ...

  20. Atividade Metanogênica Específica (AME) de lodos industriais provenientes do tratamento biológico aeróbio e anaeróbio

    OpenAIRE

    Schneiders,Danieli; Silva,Joel Dias da; Till,Aline; Lapa,Katt Regina; Pinheiro,Adilson

    2013-01-01

    Nesse estudo procederam-se testes de atividade metanogênica específica (AME) para lodo de indústria têxtil e lodo de indústria alimentícia. O lodo têxtil (aeróbio), proveniente de um sistema de lodos ativados, foi coletado na entrada do decantador biológico secundário e o lodo alimentício (anaeróbio) foi coletado em um reator UASB. Uma vez coletados, os lodos foram caracterizados e submetidos a ensaios de AME. Constatou-se que os microrganismos presentes no lodo alimentício apresentaram AME d...